Interactions between ATP and cholesterol side-chain cleavage in mitochondria isolated from superovulated rat ovaries.
ATP stimulated the rate of [4-14C]cholesterol side-chain cleavage in mitochondria isolated from superovulated rat ovaries. The effect of ATP was apparently similar to the stimulatory effect of choriogonadotropin on mitochondrial [4-14C]cholesterol utilization. Enhancement of the rate of steroidogenesis by ATP and choriogonadotropin were not additive. ATP seemed to promote both cholesterol uptake into the inner mitochondrial membrane and the supply of electrons for [4-14C]cholesterol utilization from both endogenous substrate and succinate.
D019297:2,4-Dinitrophenol; D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000818:Animals; D002250:Carbon Radioisotopes; D002784:Cholesterol / Q000378:metabolism*; D000242:Cyclic AMP / Q000032:analysis; D004140:Dinitrophenols / Q000494:pharmacology; D005260:Female; D007425:Intracellular Membranes / Q000378:metabolism; D007523:Isocitrates / Q000494:pharmacology; D008928:Mitochondria / Q000378:metabolism* / Q000648:ultrastructure; D009249:NADP / Q000494:pharmacology; D010053:Ovary / Q000648:ultrastructure*; D051381:Rats; D011919:Rats, Inbred Strains; D013386:Succinates / Q000494:pharmacology; D013480:Superovulation
D002250:Carbon Radioisotopes; D004140:Dinitrophenols; D007523:Isocitrates; D013386:Succinates; D009249:NADP; D000255:Adenosine Triphosphate; D002784:Cholesterol; D000242:Cyclic AMP; D019297:2,4-Dinitrophenol