Hormonal regulation of collagen synthesis in a clonal rat osteosarcoma cell line.
Collagen synthesis in rat osteosarcoma cell line 17/2 (ROS 17/2) was assessed by measuring the incorporation of [3H]proline into collagenase-digestible protein and the formation of [3H]hydroxyproline. PTH and 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3] inhibited collagen synthesis in ROS 17/2 cells in a time- and dose-dependent manner. PTH reduced collagen synthesis after a 3-h incubation, whereas the effect of 1,25-(OH)2D3 was somewhat slower. Maximal and half-maximal inhibition of collagen synthesis occurred at approximately 1 and 0.1 nM of each hormone, respectively. At confluency, ROS 17/2 cells synthesized 96% type I and 4% type III collagen. PTH reduced the synthesis of type I, but not type III, collagen. PTH and 1,25-(OH)2D3 also reduced procollagen mRNA levels, as determined by a dot blot hybridization assay. Thus, ROS 17/2 cells are a convenient model system for studying the hormonal regulation of collagen metabolism and gene expression in a cloned cell line with the osteoblastic phenotype.
D000818:Animals; D002117:Calcitriol / Q000494:pharmacology; D002460:Cell Line; D002999:Clone Cells / Q000378:metabolism; D003094:Collagen / Q000096:biosynthesis* / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D005786:Gene Expression Regulation; D006909:Hydroxyproline / Q000378:metabolism; D003012:Microbial Collagenase / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D012516:Osteosarcoma / Q000378:metabolism*; D010281:Parathyroid Hormone / Q000494:pharmacology; D011347:Procollagen / Q000235:genetics; D011392:Proline / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D013997:Time Factors
D010281:Parathyroid Hormone; D011347:Procollagen; D012333:RNA, Messenger; D003094:Collagen; D011392:Proline; D003012:Microbial Collagenase; D002117:Calcitriol; D006909:Hydroxyproline