Serological characterization of human reassortant rotaviruses.
We analyzed the serological properties of two human wild-type cell culture-adapted rotaviruses (strains 308 and 46) and of 308 X 46 reassortants which were previously obtained and genetically characterized. Strain 308, exhibiting a so-called long RNA pattern, was found to belong to human rotavirus subgroup II, serotype 1, whereas strain 46, exhibiting a so-called short RNA pattern, represented subgroup I, serotype 2. Among the 308 X 46 reassortants we analyzed, two belonged to subgroup II, serotype 1, and exhibited short RNA patterns. This showed that the correlation observed between human subgroups I and II rotaviruses and the short and long electrophoretic patterns is not supported by any molecular basis (i.e., gene segment 10 or 11 was not involved in the subgroup specificity).
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral / Q000235:genetics; D011232:Chemical Precipitation; D006801:Humans; D007158:Immunologic Techniques; D012367:RNA, Viral / Q000032:analysis; D011995:Recombination, Genetic; D012401:Rotavirus / Q000145:classification* / Q000235:genetics / Q000276:immunology; D012703:Serotyping
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D012367:RNA, Viral