Establishment of mutant FM3A murine mammary carcinoma cell strains transformed with the herpes simplex virus type 1 thymidine kinase gene.
To establish cell systems appropriate for investigating the mode of action of anti-herpetic nucleoside analogs, mutants were constructed from murine FM3A mammary carcinoma cells, which were deficient in both thymidine kinase (EC 2.7.1.21) and thymidylate synthase (EC 2.1.1.45), but were transformed with a recombinant plasmid DNA containing the herpes simplex virus type 1 thymidine kinase gene. The transformed cells expressed viral thymidine kinase activity and showed unusually high sensitivity to the cytostatic action of the antiherpetic agent (E)-5-(2-iodovinyl)-2'-deoxyuridine, which was only weakly inhibitory to the growth of the parent cells.
D000818:Animals; D002460:Cell Line; D002472:Cell Transformation, Viral; D004274:DNA, Recombinant; D005796:Genes; D005814:Genes, Viral; D008325:Mammary Neoplasms, Experimental / Q000235:genetics*; D051379:Mice; D008817:Mice, Mutant Strains; D010957:Plasmids; D018139:Simplexvirus / Q000235:genetics*; D013937:Thymidine Kinase / Q000235:genetics*; D013940:Thymidylate Synthase / Q000235:genetics
D004274:DNA, Recombinant; D013940:Thymidylate Synthase; D013937:Thymidine Kinase