A poliovirus temperature-sensitive RNA synthesis mutant located in a noncoding region of the genome.
We have constructed an 8-base-pair insertion mutation in the 3' noncoding region of an infectious poliovirus cDNA clone that gives rise to a temperature-sensitive RNA synthesis mutant upon transfection into mammalian cells. The mutated cDNA was used to establish a cell line that releases the mutant poliovirus in a temperature-dependent fashion, representing a unique persistent viral infection. A poliovirus mutant mapping in the noncapsid region of the viral genome can be complemented in this cell line, implying that the cell line expresses viral proteins at the nonpermissive temperature.
D001483:Base Sequence; D004247:DNA / Q000378:metabolism; D004251:DNA Transposable Elements; D005814:Genes, Viral; D005816:Genetic Complementation Test; D006367:HeLa Cells; D006801:Humans; D009154:Mutation; D010641:Phenotype; D017955:Poliovirus / Q000235:genetics*; D012367:RNA, Viral / Q000096:biosynthesis*; D013696:Temperature
D004251:DNA Transposable Elements; D012367:RNA, Viral; D004247:DNA