[Characteristics of the subvirion components formed after the treatment of Machupo virus with a nonionic detergent].
Machupo virus labeled with radioactive precursors of RNA or protein synthesis was purified by isodensity ultracentrifugation in sucrose concentration gradient. The purified virus was disrupted with NP-40 nonionic detergent in the presence of 1 M KCl, and subvirion fractions were separated by ultracentrifugation in urografin density gradient. This treatment resulted in formation of two subvirion components with a buoyant density 1.25-1.26 and 1.10-1.12 g/cm3 in urografin concentration gradient. The subvirion fraction with the density of 1.25-1.26 g/cm3 contained high-molecular virion RNAs, a major protein of molecular weight of 64 kD, and seemed to be the virion nucleocapsid. The solubilised fraction (1.10-1.12 g/cm3) contained glycosylated protein 37 kD which seems to be a surface glycoprotein.
D001116:Arenaviridae / Q000187:drug effects*; D006477:Arenaviruses, New World / Q000187:drug effects* / Q000302:isolation & purification; D001798:Blood Proteins / Q000032:analysis / Q000302:isolation & purification; D002499:Centrifugation, Density Gradient / Q000379:methods; D003902:Detergents / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D047091:Eosinophil Granule Proteins; D008970:Molecular Weight; D012367:RNA, Viral / Q000032:analysis / Q000302:isolation & purification; D012260:Ribonucleases; D013501:Surface-Active Agents / Q000494:pharmacology*; D014461:Ultracentrifugation; D014771:Virion / Q000187:drug effects* / Q000302:isolation & purification
D001798:Blood Proteins; D003902:Detergents; D047091:Eosinophil Granule Proteins; D012367:RNA, Viral; D013501:Surface-Active Agents; D012260:Ribonucleases
Kharakteristika subvirionnykh komponentov, obrazuiushchikhsia posle obrabotki virusa Machupo neionnym detergentom.