Cloning of the Escherichia coli K-12 guaC gene following its transposition into the RP4::Mu cointegrate.
The guanosine 5'-monophosphate reductase gene, guaC, has been cloned into the multicopy vector pBR325 from the RP4::Mu cointegrate, pKGM62-1, and the gene product identified by in vitro transcription/translation as a protein of Mr 36 000. Strains harbouring the recombinant plasmid had increased levels of GMP-reductase activity.
D002872:Chromosome Deletion; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D050899:GMP Reductase; D005796:Genes; D005798:Genes, Bacterial; D005822:Genetic Vectors; D005838:Genotype; D009247:NADH, NADPH Oxidoreductases / Q000235:genetics*; D010957:Plasmids
D004251:DNA Transposable Elements; D009247:NADH, NADPH Oxidoreductases; D050899:GMP Reductase; D004262:DNA Restriction Enzymes