Immunological detection of cauliflower mosaic virus gene V protein produced in engineered bacteria or infected plants.
Antiserum was prepared against a synthetic peptide corresponding to the C-terminal 25 amino acids (aa) of the protein encoded by cauliflower mosaic virus (CaMV) gene V, which is thought to be a reverse transcriptase involved in viral DNA replication. This antiserum was used to detect the expression of CaMV gene V either in Escherichia coli JM103 transformed by an expression vector containing CaMV gene V or in CaMV-infected plants. In both cases, an 80-kDal protein has been detected.
D000595:Amino Acid Sequence; D001483:Base Sequence; D001937:Brassica; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D005796:Genes; D005814:Genes, Viral; D005818:Genetic Engineering; D007106:Immune Sera; D009029:Mosaic Viruses / Q000235:genetics*; D010944:Plants / Q000235:genetics*; D014764:Viral Proteins / Q000032:analysis* / Q000235:genetics / Q000276:immunology
D004279:DNA, Viral; D007106:Immune Sera; D014764:Viral Proteins; D004262:DNA Restriction Enzymes