Use of the human liver cell line Hep G2 in a modified Salmonella reversion assay.
The human hepatoma cell line Hep G2 was used to activate promutagenic chemicals to mutagens in a modified Salmonella typhimurium reversion assay. Hep G2 cells mediated positive mutagenic responses in tester strain TA98 with 5 and 25 micrograms/plate of 2-aminofluorene, but these responses were consistently lower than those seen using primary rat hepatocytes. In addition, 3 and 6 X 10(6) Hep G2 cells per assay produced positive mutagenic responses with 2-aminoanthracene, benzidine, acetylbenzidine and aflatoxin B1, while benzo[a]pyrene, 7,12-dimethylbenz[a]anthracene, 3-methylcholanthrene, 4-aminobiphenyl and 4- and 11-aminobenzo[a]pyrene were nonmutagenic with Hep G2-cell activation. These results indicate that Hep G2 cells may be a useful intact cellular metabolizing system of human origin for predicting the genotoxicity of promutagenic agents, but that the use of Salmonella as a target cell may limit the classes of mutagens detected.
D001711:Biotransformation; D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D002460:Cell Line; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism*; D009152:Mutagenicity Tests; D009153:Mutagens / Q000378:metabolism; D012475:Salmonella / Q000187:drug effects
D009153:Mutagens