Production of guanidine-resistant and -dependent poliovirus mutants from cloned cDNA: mutations in polypeptide 2C are directly responsible for altered guanidine sensitivity.
cDNA fragments representing the region in polypeptide 2C containing mutations in a guanidine-resistant or -dependent mutant were cloned into the wild-type background of an infectious clone. Transfection of COS-1 cells with these plasmids yielded viruses that were either completely resistant to 2.0 mM guanidine hydrochloride or dependent on this concentration of drug for growth.
D002460:Cell Line; D003001:Cloning, Molecular; D004247:DNA; D004352:Drug Resistance, Microbial; D005814:Genes, Viral; D019791:Guanidine; D006146:Guanidines / Q000494:pharmacology*; D009154:Mutation; D010455:Peptides / Q000235:genetics; D017955:Poliovirus / Q000187:drug effects / Q000235:genetics*; D014162:Transfection; D014764:Viral Proteins / Q000235:genetics*
D006146:Guanidines; D010455:Peptides; D014764:Viral Proteins; D004247:DNA; D019791:Guanidine