Measurement of leukotriene B4 in vitro and in vivo by radioimmunoassay.
The concentration of LTB4 in in vitro incubations of leukocytes and in experimentally-induced inflammatory exudate can be determined reliably by direct RIA; i.e., extraction and chromatography of samples prior to RIA are not mandatory. It is probable that the RIA for LTB4 is also suitable for monitoring the levels of LTB4 in clinical samples (e.g., synovial fluid, blister fluid from involved psoriatic skin).
D000818:Animals; D006801:Humans; D007249:Inflammation; D007975:Leukotriene B4 / Q000032:analysis* / Q000097:blood; D009504:Neutrophils / Q000378:metabolism; D011863:Radioimmunoassay / Q000379:methods; D012710:Serum Albumin, Bovine
D007975:Leukotriene B4; D012710:Serum Albumin, Bovine