Nuclear location signals in polyoma virus large-T.
We have found two mutually independent sequence elements that contribute to the nuclear location of polyoma virus large-T. The first sequence (pro lys lys282 ala arg glu asp) resembles the SV40 large-T nuclear signal (pro lys lys128 lys arg lys val) and occurs at a corresponding position within polyoma large-T. The second sequence (val ser arg lys192 arg pro arg) may be structurally related to the SV40 signal, although it has little sequence homology and falls in a region of the protein that has no counterpart in SV40 large-T. The data suggest that nuclear location signals with characteristics similar to the SV40 large-T prototype may be a more general feature of nuclear proteins, and that several such signals in a given protein can exert cooperative effects.
D000595:Amino Acid Sequence; D000818:Animals; D000952:Antigens, Polyomavirus Transforming; D000957:Antigens, Viral, Tumor / Q000235:genetics / Q000378:metabolism*; D001692:Biological Transport; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism*; D002522:Chlorocebus aethiops; D002872:Chromosome Deletion; D007668:Kidney; D009856:Oncogene Proteins, Viral / Q000235:genetics / Q000378:metabolism*; D011120:Polyomavirus / Q000235:genetics / Q000378:metabolism*; D012689:Sequence Homology, Nucleic Acid; D013539:Simian virus 40 / Q000235:genetics
D000952:Antigens, Polyomavirus Transforming; D000957:Antigens, Viral, Tumor; D009856:Oncogene Proteins, Viral