Metabolic differentiation of red and white skeletal muscle in vivo and in monolayer culture.
Cytochrome oxidase, succinate oxidase and lactate dehydrogenase were compared in: (a) leg and breast muscle from 11-19-day-old chick embryos; and (b) 2, 6, 10 and 14-day-old primary cell cultures established from myoblasts of embryonic leg and breast muscle. Cytochrome oxidase, succinate oxidase and lactate dehydrogenase activities were higher (48.8, 65.4, 277.6%, respectively) in leg muscle after 19 days in ovo. Cytochrome and succinate oxidase activities were higher (111.3, 48.1%, respectively) in leg muscle cell cultures after 14 days in vitro. The data represent evidence for intrinsic developmental patterns for certain enzymes.
D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D002642:Chick Embryo; D003576:Electron Transport Complex IV / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D009132:Muscles / Q000166:cytology / Q000378:metabolism*; D013385:Succinate Dehydrogenase / Q000378:metabolism; D013997:Time Factors
D007770:L-Lactate Dehydrogenase; D013385:Succinate Dehydrogenase; D003576:Electron Transport Complex IV