The nature of ompA mutants of Escherichia coli K12 exhibiting temperature-sensitive bacteriophage resistance.
A class of ompA mutants of Escherichia coli, exhibiting temperature-sensitive resistance towards phages using the OmpA protein as receptor, was analysed. The mutants produce detectable levels of the protein at 42 degrees C but not at 30 degrees C (Manning and Reeves 1976). They were found to have a deletion (one isolate) or insertions (three isolates) upstream of the coding part of the ompA gene. Several previously characterized mutants possessing insertions or a deletion in the non-translated 5' area of the gene also exhibited a similar temperature-sensitive phage resistance. This cold-sensitive phenotype is explained in terms of the recent discovery that the stability of ompA mRNA is regulated by the rate of cell growth (Nilsson et al. 1984).
D001425:Bacterial Outer Membrane Proteins / Q000235:genetics* / Q000302:isolation & purification; D003090:Coliphages / Q000235:genetics*; D004262:DNA Restriction Enzymes; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics*; D008970:Molecular Weight; D009154:Mutation; D009693:Nucleic Acid Hybridization; D013696:Temperature
D001425:Bacterial Outer Membrane Proteins; D004251:DNA Transposable Elements; D004262:DNA Restriction Enzymes