Partial purification and biological properties of an eosinophil-activating factor.
A protein (eosinophil-activating factor, EAF), which enhances the capacity of human peripheral blood eosinophils to kill antibody-coated schistosomula of Schistosoma mansoni, has been partially purified from supernatants of cultured peripheral blood mononuclear cells by sequential chromatography on Sephacryl S200 and DEAE-cellulose. This protein is acidic with a molecular mass on gel filtration of 40 +/- 7 kDa. It not only enhances the activity of eosinophils against schistosomula but also increases their ability to lyse antibody-coated, herpes simplex virus-infected Chang liver cells. It enhances the production of superoxide and hydrogen peroxide by eosinophils that occurs both spontaneously and in response to opsonized zymosan. However, increased respiratory burst activity does not appear to be responsible for the enhancement of eosinophil-mediated killing of schistosomula, since a comparable or greater increase in hydrogen peroxide production is induced by column fractions that have little or no effect on schistosomulum killing. EAF enhances eosinophil degranulation, both spontaneously and after incubation with opsonized zymosan. Enhanced degranulation is associated with release of eosinophil peroxidase and eosinophil cationic protein. These findings suggest that EAF enhances the capacity of eosinophils to kill parasites by increasing the extent of eosinophil degranulation and the amount of toxic granule proteins that are secreted.
D000920:Antibody-Dependent Cell Cytotoxicity; D001798:Blood Proteins / Q000378:metabolism; D002448:Cell Adhesion; D003594:Cytoplasmic Granules / Q000378:metabolism / Q000502:physiology; D047091:Eosinophil Granule Proteins; D004804:Eosinophils / Q000276:immunology* / Q000378:metabolism / Q000502:physiology; D006801:Humans; D006861:Hydrogen Peroxide / Q000096:biosynthesis; D007527:Isoenzymes / Q000378:metabolism; D008222:Lymphokines / Q000302:isolation & purification* / Q000502:physiology; D009195:Peroxidase; D010544:Peroxidases / Q000378:metabolism; D012260:Ribonucleases; D012550:Schistosoma mansoni / Q000276:immunology; D013481:Superoxides / Q000378:metabolism
D001798:Blood Proteins; D047091:Eosinophil Granule Proteins; D007527:Isoenzymes; D008222:Lymphokines; C022227:eosinophil stimulating promoter; D013481:Superoxides; D006861:Hydrogen Peroxide; D010544:Peroxidases; D009195:Peroxidase; D012260:Ribonucleases