Direct method for quantitation of extreme polymerase error frequencies at selected single base sites in viral RNA.
Methods are described which allow direct quantitation and sequence analysis of base substitution levels at predetermined single nucleotide positions in cloned pools of an RNA virus genome or in its RNA transcripts in vitro. Base substitution frequencies for vesicular stomatitis virus (VSV) at one highly conserved site examined were reproducible and extremely high, averaging between 10(-4) and 4 X 10(-4) substitutions per base incorporated at this single site. If polymerase error frequencies averaged as high at all other sites in the 11-kilobase VSV genome, then every member of a cloned VSV population would differ from most other genomes in that clone at a number of different nucleotide positions. The preservation of a consensus sequence in such variable RNA virus genomes then could only result from strong biological selection (in a single host or multihost environment) for the most fit and competitive representatives of extremely heterogeneous virus populations.
D001483:Base Sequence; D012321:DNA-Directed RNA Polymerases / Q000502:physiology*; D003673:Defective Viruses / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D008722:Methods; D009154:Mutation; D009841:Oligonucleotides / Q000032:analysis; D012316:RNA Nucleotidyltransferases / Q000502:physiology*; D012333:RNA, Messenger / Q000032:analysis*; D012367:RNA, Viral / Q000032:analysis*; D012324:RNA-Dependent RNA Polymerase / Q000502:physiology*; D012641:Selection, Genetic; D014721:Vesicular stomatitis Indiana virus / Q000235:genetics
D009841:Oligonucleotides; D012333:RNA, Messenger; D012367:RNA, Viral; D012316:RNA Nucleotidyltransferases; D012324:RNA-Dependent RNA Polymerase; D012321:DNA-Directed RNA Polymerases