Possible relationship between antigenic properties and isolation history of HSV-1 strains.
29 monoclonal anti-herpes simplex virus (HSV)-1 antibodies were produced and characterized with regard to virus neutralizing activity, intracellular or cell-surface location of viral antigens and, where possible, molecular weight of the viral protein recognized. 13 antibodies recognized viral antigens expressed on the surface of infected cells and 16 were directed to intracellular viral components. Only two antibodies exhibited virus neutralizing activity. Application of these antibodies to an antigenic comparison of standard laboratory HSV-1 strains F, HFEM, mP, Glasgow-17 and MAC revealed unique antigenic differences among these strains. The antibodies were further used in an antigenic comparison of 45 human HSV-1 isolates with defined isolation history. Except for two paired isolates from left and right trigeminal ganglia of two human cadavers, the antibody panel revealed antigenic differences among all isolates, including paired isolates from three additional cadavers. Overall, isolates from different human donors showed greater antigenic dissimilarity from each other in cell-surface associated than in intracellular antigens. The data suggest the possibility of a correlation between antigenic and biologic properties of HSV-1.
D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D000956:Antigens, Viral / Q000032:analysis*; D002294:Carcinoma, Squamous Cell; D002460:Cell Line; D006224:Cricetinae; D005006:Ethnicity; D005260:Female; D006561:Herpes Simplex / Q000382:microbiology; D006801:Humans; D007668:Kidney; D008297:Male; D044469:Racial Groups; D011863:Radioimmunoassay; D018139:Simplexvirus / Q000276:immunology* / Q000302:isolation & purification
D000954:Antigens, Surface; D000956:Antigens, Viral