Resistance of horse alpha 1-proteinase inhibitor to perchloric acid denaturation and a simplified purification procedure resulting therefrom.
Addition of perchloric acid (6.4% w/v final concentration) to horse alpha 1-proteinase inhibitor or to horse plasma neither precipitated nor inactivated alpha 1-proteinase inhibitor. None of the isoinhibitors of alpha 1-proteinase inhibitor was altered by dilute perchloric acid. This unexpected behavior led to a simplified procedure for the purification of horse alpha 1-proteinase inhibitor, consisting of removal of the bulk of plasma proteins, by perchloric acid precipitation and by gel filtration on Sephadex G-75 and G-200. The resulting preparations of alpha 1-proteinase inhibitor were immunogenically pure. The simplified purification procedure permitted the immunochemical comparison of the isoinhibitors of alpha 1-proteinase inhibitor, which proved to be immunologically identical.
D000818:Animals; D001798:Blood Proteins / Q000302:isolation & purification; D006736:Horses; D007122:Immunoelectrophoresis; D008970:Molecular Weight; D010472:Perchlorates / Q000494:pharmacology*; D011480:Protease Inhibitors / Q000097:blood; D011489:Protein Denaturation; D014357:Trypsin / Q000378:metabolism; D000515:alpha 1-Antitrypsin
D001798:Blood Proteins; D010472:Perchlorates; D011480:Protease Inhibitors; D000515:alpha 1-Antitrypsin; D014357:Trypsin