Nucleotide sequence of exon I of the rat c-K-ras gene.
We have isolated a DNA fragment containing exon I of rat cellular proto-oncogene of K-ras and sequenced the exon I. The obtained sequence was compared with that of the corresponding region in viral oncogene of Kirsten strain of murine sarcoma virus (Ki-MSV). The results showed that the exon I sequences of cellular K-ras genes in rat and human cells could encode the same amino acid sequence, while the viral K-ras gene could code for two different amino acids corresponding to the 12th and 37th positions from the N-terminus of K-ras gene product. The amino acid substitutions found between viral and cellular K-ras genes are discussed in relation to the transforming ability of Ki-MSV.
D000818:Animals; D001483:Base Sequence; D003062:Codon / Q000235:genetics; D004247:DNA / Q000235:genetics; D004262:DNA Restriction Enzymes; D006801:Humans; D009693:Nucleic Acid Hybridization; D009857:Oncogenes; D000090063:Proto-Oncogene Mas; D011519:Proto-Oncogenes; D051381:Rats; D011919:Rats, Inbred Strains
D003062:Codon; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D004247:DNA; D004262:DNA Restriction Enzymes