Characterization of human genomic DNA sequences homologous to the interleukin 2 cDNA.
Southern blot analysis of human placental DNA under low stringency hybridization conditions revealed several DNA fragments hybridizable to the human interleukin 2 (IL-2) cDNA. Four phage clones carrying these IL-2 cDNA-like sequences were isolated and their structures analyzed. A DNA fragment derived from one of the clones gave the strongest hybridization signal. Sequence analysis of this fragment revealed the presence of a cluster of three DNA segments, i.e. 20 base pairs (bp), 57 bp and 18 bp in length and having about 85%, 80% and 83% homology to three different parts of the coding region of human IL-2 cDNA, respectively.
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis / Q000235:genetics; D004262:DNA Restriction Enzymes; D005260:Female; D005796:Genes; D006801:Humans; D007376:Interleukin-2 / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D012689:Sequence Homology, Nucleic Acid
D007376:Interleukin-2; D004247:DNA; D004262:DNA Restriction Enzymes