Morphine-mimetic anti-paratypic antibodies: cross-reactive properties.
Polyclonal, anti-idiotypic, rabbit antibodies have been raised against four murine monoclonal anti-morphine Fab fragments. The antibody preparations, after affinity purification, have been shown to contain an anti-paratypic fraction which reversibly inhibits morphine binding to the anti-morphine antibodies and to cellular opiate receptors. Using some of the unique properties of this system, for the first time cross-reactivities of anti-paratypic antibodies with the monoclonal anti-morphine IgGs have been examined including competition for the same binding sites by a classical opiate agonist/antagonist pair.
D000818:Animals; D000911:Antibodies, Monoclonal; D001667:Binding, Competitive; D002846:Chromatography, Affinity; D003429:Cross Reactions; D005048:Etorphine / Q000378:metabolism; D007140:Immunoglobulin Fab Fragments / Q000276:immunology; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D051379:Mice; D009020:Morphine / Q000276:immunology*; D009270:Naloxone / Q000378:metabolism; D011817:Rabbits; D011957:Receptors, Opioid / Q000276:immunology / Q000378:metabolism
D000911:Antibodies, Monoclonal; D007140:Immunoglobulin Fab Fragments; D007130:Immunoglobulin Idiotypes; D011957:Receptors, Opioid; D009270:Naloxone; D005048:Etorphine; D009020:Morphine