Specific transcription of orthopox virus DNA by HeLa cell RNA polymerase II.
A HeLa cell extract was used to transcribe DNA isolated from cowpox virus. Truncated templates generate accurately initiated run-off transcripts of discrete sizes and whose sensitivity to inhibition by alpha-amanitin indicates synthesis by cell RNA polymerase II. A mapped restriction fragment of wild-type cowpox DNA contains specific sites of initiation which are not detected in the geographically equivalent fragment from a cowpox mutant having a defined sequence rearrangement in this region.
D000546:Amanitins / Q000494:pharmacology; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant / Q000378:metabolism; D004279:DNA, Viral / Q000378:metabolism*; D006367:HeLa Cells / Q000201:enzymology*; D006801:Humans; D009154:Mutation; D010957:Plasmids; D012319:RNA Polymerase II / Q000378:metabolism*; D014158:Transcription, Genetic / Q000187:drug effects; D014616:Vaccinia virus / Q000235:genetics*
D000546:Amanitins; D004274:DNA, Recombinant; D004279:DNA, Viral; D012319:RNA Polymerase II; D004262:DNA Restriction Enzymes