Effect of nerve growth factor on glucose utilization and nucleotide content of pheochromocytoma cells (clone PC12).
The effect of nerve growth factor (NGF) on the utilization and fate of uniformly labeled 14C glucose and on the content of several pyridine and purine nucleotides has been tested in the clonal cell line PC12. After incubation for 72 h with NGF, PC12 cells exhibit a 2.7-fold increase in glucose utilization and a 4.7-fold increase in CO2 release. During the same incubation period, all the nucleotides tested (NAD+, AMP, GMP, UDP-glucose, UDP-galactose, UDP, ADP, GDP, UTP, CTP, ATP, and GTP) underwent significant increments, varying from a minimum of 27% for ADP to a maximum of 90-120% for AMP, GMP, UDP-glucose, and UDP-galactose. These findings are discussed in connection with the trophic and differentiative effects of NGF in PC12 cells, which, in the presence of this factor, shifted from a neoplastic to a neuronal-like cell population.
D000249:Adenosine Monophosphate / Q000378:metabolism; D000310:Adrenal Gland Neoplasms / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D003570:Cytidine Triphosphate / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D006157:Guanosine Monophosphate / Q000378:metabolism; D009414:Nerve Growth Factors / Q000494:pharmacology*; D009711:Nucleotides / Q000378:metabolism*; D010673:Pheochromocytoma / Q000378:metabolism*; D051381:Rats; D014531:Uridine Diphosphate Galactose / Q000378:metabolism; D014532:Uridine Diphosphate Glucose / Q000378:metabolism; D014544:Uridine Triphosphate / Q000378:metabolism
D009414:Nerve Growth Factors; D009711:Nucleotides; D014531:Uridine Diphosphate Galactose; D000249:Adenosine Monophosphate; D003570:Cytidine Triphosphate; D006157:Guanosine Monophosphate; D005947:Glucose; D014544:Uridine Triphosphate; D014532:Uridine Diphosphate Glucose