title: Sequence of the D beta 2-J beta 2 region of the human T-cell receptor beta-chain locus.
abstract: We have sequenced the region encompassing a D beta 2 segment and the J beta 2 segments of the human T-cell antigen receptor beta-chain genes. The D beta 2 element lies about 650 base pairs upstream of a cluster of seven potentially functional J beta 2 sequences and one J beta 2 pseudogene. Examination of human beta-chain cDNA sequences which involve rearranged D beta 2 and J beta 2 elements demonstrates that N-region, as well as junctional, diversity can occur during D-J joining. Further, we present evidence for possible somatic mutation in active J beta 2 segments.
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis; D004262:DNA Restriction Enzymes; D005796:Genes; D005822:Genetic Vectors; D006801:Humans; D046911:Macromolecular Substances; D051379:Mice; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*; D013045:Species Specificity
chemical_list: D046911:Macromolecular Substances; D011948:Receptors, Antigen, T-Cell; D004247:DNA; D004262:DNA Restriction Enzymes