Improvement of a simple method to purify ribonucleotide reductase.
The use of an ATP-agarose column to purify ribonucleotide reductase from human D-98 cells was recently reported. The column selectively retains greater than 99.9% of the contaminating nucleoside diphosphate (NDP) kinase from crude preparations of ribonucleotide reductase. It was presently found, however, that extending the length of the column caused the ribonucleotide reductase to dissociate into subunits. One subunit appeared in the low ionic strength buffer wash while the other required 0.5 M KCl for elution. The enzyme could also be recovered intact (non-dissociated) by equilibrating the enzyme preparation and the column with 0.5 M KCl prior to chromatography. Either method greatly improved the overall yield and the specific activity of the ribonucleotide reductase because it prevented the binding and subsequent loss of any of the subunits. In addition, the use of a larger column permitted the gel-filtration properties of the ATP-agarose to separate the bulk of the residual (not bound) NDP kinase from the ribonucleotide reductase.
D002846:Chromatography, Affinity; D006801:Humans; D046911:Macromolecular Substances; D009701:Nucleoside-Diphosphate Kinase / Q000302:isolation & purification; D012264:Ribonucleotide Reductases / Q000302:isolation & purification*
D046911:Macromolecular Substances; D012264:Ribonucleotide Reductases; D009701:Nucleoside-Diphosphate Kinase