Dipyridamole-induced interferon production in mouse peritoneal leukocytes.
The in vitro explanted mouse peritoneal leukocytes were used for the optimization of the dipyridamole-induced interferon production. After 90-120 min incubation of cells with 30-100 microM dipyridamole, the production of interferon reached 6.4 X 10(4) IU/ml. It was demonstrated that the interferon production phase is preceded by the dipyridamole-dependent increase in cAMP concentration. The possibility of cAMP involvement in the mechanism of interferon production is being discussed.
D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000037:antagonists & inhibitors; D000818:Animals; D000242:Cyclic AMP / Q000378:metabolism; D004176:Dipyridamole / Q000494:pharmacology*; D066298:In Vitro Techniques; D007369:Interferon Inducers; D007700:Kinetics; D007962:Leukocytes / Q000201:enzymology / Q000378:metabolism*; D051379:Mice; D010537:Peritoneum / Q000378:metabolism
D007369:Interferon Inducers; D004176:Dipyridamole; D000242:Cyclic AMP; D015105:3',5'-Cyclic-AMP Phosphodiesterases