title: [A macrophage migration-inhibiting factor in preparations of alpha- and gamma-interferon].
abstract: Native preparations of human alpha- and gamma-interferon synthesized in stationary, suspension or roller leukocyte cultures regularly showed high levels of migration inhibition factor (MIF) activity a significant portion of which was lost in the course of interferon purification and concentration. Minimal losses of MIF activity were achieved when medicinal preparations of interferon were made by the method of negative immunoabsorption on immobilized antibodies to inductor antigens and chromatography on Sephadex G-25.
mesh_terms: D000818:Animals; D004340:Drug Contamination; D006801:Humans; D007370:Interferon Type I / Q000096:biosynthesis / Q000302:isolation & purification* / Q000494:pharmacology; D007371:Interferon-gamma / Q000096:biosynthesis / Q000302:isolation & purification* / Q000494:pharmacology; D008263:Macrophage Migration-Inhibitory Factors / Q000302:isolation & purification / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects; D008722:Methods; D051379:Mice; D008807:Mice, Inbred BALB C; D014721:Vesicular stomatitis Indiana virus / Q000187:drug effects
chemical_list: D007370:Interferon Type I; D008263:Macrophage Migration-Inhibitory Factors; D007371:Interferon-gamma
vernacular_title: Faktor, ingibiruiushchiĭ migratsiiu makrofagov, v preparatakh al'fa- i gamma-interferona.