Differential sensitivity of two functions of the insulin receptor to the associated proteolysis: kinase action and hormone binding.
Since we observed that after purification the receptor kinase activity is rapidly lost under conditions where insulin binding function seems to be preserved, we have studied the cause(s) of receptor kinase inactivation. Highly purified placental insulin receptor preparations were analyzed by NaDodSO4/PAGE followed by silver staining or immunostaining using domain-specific antibodies raised against synthetic peptides corresponding to the amino acid sequences of the beta subunit. These studies revealed the intact 90-kDa beta subunit is degraded first to an 88-kDa form and then to a 50-kDa beta 1-subunit form by proteolysis even after purification when stored at 4 degrees C. The 88-kDa beta subunit, which lacks the carboxyl-terminal approximately equal to 2-kDa portion exhibits almost no autophosphorylation activity, nor does insulin stimulate autophosphorylation. The loss of kinase activity as measured by phosphorylation of the src-related peptide is correlated with the loss of the intact 90-kDa beta subunit. Degradation of the beta subunit to the 50-kDa form seems to be facilitated by the removal of the approximately equal to 2-kDa peptide. Present studies thus suggest that only the intact form of the beta subunit has full kinase activity in an insulin-dependent manner and that other forms, such as the 88-kDa beta subunit show little kinase activity. The inactivation appears to arise from a conformational change of the 90-kDa form, which makes it susceptible to proteolysis at the carboxyl-terminal end. These results imply that the carboxyl-terminal of the beta subunit is important for the manifestation of the tyrosine kinase activity of the insulin receptor.
D000595:Amino Acid Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007328:Insulin / Q000378:metabolism*; D010766:Phosphorylation; D011505:Protein-Tyrosine Kinases / Q000032:analysis*; D011972:Receptor, Insulin / Q000276:immunology / Q000378:metabolism*; D013329:Structure-Activity Relationship; D014357:Trypsin / Q000494:pharmacology
D007328:Insulin; D011505:Protein-Tyrosine Kinases; D011972:Receptor, Insulin; D014357:Trypsin