title: Amplification of the ArgF region in strain HfrP4X of E. coli K-12.
abstract: In E. coli K-12 the argF gene is flanked by ISI sequences in direct repeat. Mutants that overproduce the argF-coded enzyme ornithine transcarbamylase can be selected; we have shown that in one class of these mutants there is an approximately forty five-fold amplification of the region bounded by the ISI repeats. This class of mutants has been detected only in strains in which the F-factor is integrated in cis to the region.
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005784:Gene Amplification; D005796:Genes; D005798:Genes, Bacterial; D009954:Ornithine Carbamoyltransferase / Q000235:genetics*; D010957:Plasmids
chemical_list: D009954:Ornithine Carbamoyltransferase; D004262:DNA Restriction Enzymes