title: Interaction of plasma gelsolin with ADP-actin.
abstract: In the presence of Ca2+, gelsolin forms a very tight, stoichiometric complex with 2 molecules of ADP-G-actin. Removal of free Ca2+ causes the 1:2 complex to dissociate to a 1:1 complex. Gelsolin accelerates the very slow polymerization of ADP-actin, apparently by accelerating the rate of nucleation, but the number concentration of filaments formed is probably less than the gelsolin concentration, indicating that the GA2 complex is not a true nucleus. These results are similar to those obtained for the interaction of gelsolin with ATP-G-actin. Both kinetic and equilibrium measurements demonstrate that the critical concentration of gelsolin-capped ADP-actin filaments (8 microM in 1 mM MgCl2 and 0.2 mM ADP) is the same as for the uncapped filaments, proving that the critical concentration is the same at both ends of the equilibrium polymer in ADP as predicted by theory. The association and dissociation rate constants for the addition of ADP-G-actin at the pointed end of an ADP-F-actin filament are estimated to be 4.6 X 10(4) M-1 s-1 and 0.4 s-1, respectively, about 15-fold lower than the rate constants at the barbed end.
mesh_terms: D000199:Actins / Q000494:pharmacology*; D000244:Adenosine Diphosphate / Q000031:analogs & derivatives* / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000031:analogs & derivatives; D002118:Calcium / Q000494:pharmacology; D002135:Calcium-Binding Proteins / Q000097:blood*; D004305:Dose-Response Relationship, Drug; D004533:Egtazic Acid / Q000494:pharmacology; D005453:Fluorescence; D018260:Gelsolin; D007700:Kinetics; D008840:Microfilament Proteins / Q000097:blood*; D011108:Polymers
chemical_list: C042634:ADP-G-actin; C022920:ATP-G-actin; D000199:Actins; D002135:Calcium-Binding Proteins; D018260:Gelsolin; D008840:Microfilament Proteins; D011108:Polymers; D004533:Egtazic Acid; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D002118:Calcium