Transduction of host cellular sequences by a retroviral shuttle vector.
We studied the frequencies and types of excision events which can occur with a retroviral shuttle vector containing the simian virus 40 origin of DNA replication. Analysis of the recloned vector plasmids by size and restriction enzyme mapping indicated that most contain one long terminal repeat. By hybridizing the plasmids to a mouse genomic repetitive DNA probe, we also determined that approximately 1 to 3% contain transduced cellular DNA sequences.
D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular; D004261:DNA Replication; D005822:Genetic Vectors; D051379:Mice; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D011120:Polyomavirus / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid; D012190:Retroviridae / Q000235:genetics*; D013539:Simian virus 40 / Q000235:genetics*