title: Activation of cation transport by lymphokines in B cells without induction of DNA synthesis or immunoglobulin gene transcription.
abstract: We report on an experimental model that permitted us to evaluate the biologic relevance of membrane-associated biochemical events with respect to cell proliferation and maturation, each induced by distinct sets of signals. Antigen-affinity-enriched murine B cells cultured in the presence of a proliferative signal induced by LPS showed activation of Na+/K+ ATPase and enhanced the uptake of proline, followed by RNA, protein, and DNA synthesis, without the generation of antibody. Stimulation with both the proliferative signal(s) and the maturation signal(s) derived from lymphokines of an EL-4 thymoma induced B cells to proliferate and synthesize mRNA encoding mu-chain of IgM and to mature into IgM-secreting cells. Most important, the secretory product of EL-4, in the absence of LPS, activated Na+/K+ ATPase but failed to stimulate uptake of proline and synthesis of DNA or mu-specific mRNA. A similar response was observed in splenocytes depleted of T cells and in unfractionated spleen cells. Thus a component secreted by EL-4 can induce some of the early molecular events characteristic of the proliferative response but lacks the ability to initiate blast transformation and DNA synthesis.
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D001693:Biological Transport, Active; D002645:Chickens; D004247:DNA / Q000096:biosynthesis*; D004789:Enzyme Activation; D004790:Enzyme Induction; D005260:Female; D007075:Immunoglobulin M / Q000235:genetics*; D007148:Immunoglobulin mu-Chains / Q000235:genetics; D008213:Lymphocyte Activation; D008222:Lymphokines / Q000502:physiology*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D011392:Proline / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D007075:Immunoglobulin M; D007148:Immunoglobulin mu-Chains; D008222:Lymphokines; D012333:RNA, Messenger; D004247:DNA; D011392:Proline; D000254:Sodium-Potassium-Exchanging ATPase