Calcitonin-responsive clonal cell line from porcine kidney (PK(15)) in serum-free medium.
PK(15), a homogeneous epithelial cell line from porcine kidney which was originally established through single cell cloning from PK-2a, was found to respond to [Asu1,7]eel calcitonin with an increase in adenosine 3':5'-cyclic monophosphate (cAMP) content, but do not respond to parathyroid hormone or arginine vasopressin. These cells were able to grow in a synthetic medium (a 1:1 mixture of Dulbecco's modified Eagle's MEM and Ham's F12 medium) without any supplementary factor. The medium supplemented with selenous acid, transferrin, and insulin permitted a growth rate equivalent to those in serum containing medium. When grown in the serum-free defined medium, these cells showed an increase in cAMP content in response to [Asu1,7]eel calcitonin to approximately the same degree as in the serum containing medium (10% fetal calf serum). Our present study first indicates that PK(15) cells are capable of growing in the serum-free defined medium retaining the calcitonin responsiveness of the original cells.
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001127:Arginine Vasopressin / Q000494:pharmacology; D001769:Blood; D002116:Calcitonin / Q000494:pharmacology*; D002455:Cell Division; D002460:Cell Line; D002999:Clone Cells; D003470:Culture Media; D000242:Cyclic AMP / Q000378:metabolism*; D007328:Insulin / Q000494:pharmacology; D007668:Kidney / Q000166:cytology* / Q000201:enzymology / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010281:Parathyroid Hormone / Q000494:pharmacology; D020887:Selenious Acid; D012643:Selenium / Q000494:pharmacology; D013552:Swine; D014168:Transferrin / Q000494:pharmacology
D003470:Culture Media; D007328:Insulin; D010281:Parathyroid Hormone; D014168:Transferrin; D001127:Arginine Vasopressin; D002116:Calcitonin; D000242:Cyclic AMP; D000469:Alkaline Phosphatase; D020887:Selenious Acid; D012643:Selenium