The restriction endonuclease Alu I induces chromosomal aberrations in human peripheral lymphocytes in vitro.
The restriction endonuclease Alu I (recognition site AG/CT) produces chromosomal aberrations in isolated human peripheral lymphocytes in vitro. The aberrations are of the chromosome-type when the cells are treated in G1 and of the chromatid-type when the cells are treated in late S, early G2. Additional treatment with ammonium sulphate leads to higher aberration frequencies than treatment with Alu I alone.
D000645:Ammonium Sulfate / Q000494:pharmacology; D002453:Cell Cycle; D002478:Cells, Cultured; D002842:Chromatids / Q000187:drug effects; D002869:Chromosome Aberrations; D002875:Chromosomes / Q000187:drug effects; D004262:DNA Restriction Enzymes / Q000494:pharmacology*; D015252:Deoxyribonucleases, Type II Site-Specific; D004357:Drug Synergism; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects* / Q000648:ultrastructure
D004262:DNA Restriction Enzymes; C024669:endodeoxyribonuclease AluI; D015252:Deoxyribonucleases, Type II Site-Specific; D000645:Ammonium Sulfate