[Structuro-functional organization of the SV40 virus chromosome. IV. Specific organization of regulatory sequences of the SV40 genome in the mini-chromosome].
Using previously described technique of hybridization end-labeling, we analysed nucleosomal organization of the regulatory region of SV40 minichromosome. We showed that DNAase II, in spite of certain specificity observed on the naked DNA, cut the minichromosome in a highly specific manner with the major hypersensitive site inside the enhancer. This hypersensitivity and that to micrococcal nuclease were not found when the chromosome of mature SV40 virions was tested.
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002843:Chromatin / Q000032:analysis / Q000235:genetics*; D002874:Chromosome Mapping; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000032:analysis* / Q000235:genetics; D004706:Endodeoxyribonucleases; D005814:Genes, Viral; D000882:Haplorhini; D006868:Hydrolysis; D007668:Kidney; D008836:Micrococcal Nuclease; D013539:Simian virus 40 / Q000235:genetics*
D002843:Chromatin; D004279:DNA, Viral; D004706:Endodeoxyribonucleases; D004262:DNA Restriction Enzymes; C019329:deoxyribonuclease II; D008836:Micrococcal Nuclease
Strukturno-funktsional'naia organizatsiia khromosomy virusa SV40. IV. Spetsificheskaia organizatsiia reguliatornykh posledovatel'nosteĭ genoma SV40 v sostave mini-khromosomy.