Construction of cloning, promoter-screening, and terminator-screening shuttle vectors for Bacillus subtilis and Streptococcus lactis.
Shuttle vectors have been constructed which are suitable both for the selection of regulatory sequences and for gene cloning in Bacillus subtilis and Streptococcus lactis. The promoter screening vectors contain a promoterless chloramphenicol acetyltransferase gene; the insertion of suitable DNA fragments upstream of the gene restored the enzyme activity. With a related set of vectors, transcription termination signals can be selected.
D000123:Acetyltransferases / Q000235:genetics; D001412:Bacillus subtilis / Q000235:genetics*; D015500:Chloramphenicol O-Acetyltransferase; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D005798:Genes, Bacterial; D005809:Genes, Regulator; D005822:Genetic Vectors; D013294:Lactococcus lactis / Q000235:genetics*; D011401:Promoter Regions, Genetic; D013728:Terminator Regions, Genetic
D000123:Acetyltransferases; D015500:Chloramphenicol O-Acetyltransferase; D004262:DNA Restriction Enzymes