Mouse cells surviving polyoma virus infection generally retain the whole viral genome.
Permissive mouse 3T6 cells were exposed to polyoma virus--either wild-type or early mutant--at high multiplicities of infection. From colonies arising from surviving cells, so-called lines and clones were derived under conditions precluding superinfection. These lines and clones were examined for the presence of viral genetic information, using a variety of techniques. Two salient findings were made: most lines or clones analyzed had retained viral genetic material; generally, this material was nondefective, as evidenced by the production of virus and/or viral DNA molecules of genomic size. These findings indicate that mouse cells can survive for many generations while carrying a complete, infectious, and potentially cytocidal polyoma virus genome.
D000818:Animals; D002470:Cell Survival; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D002999:Clone Cells; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000235:genetics; D005814:Genes, Viral; D051379:Mice; D009154:Mutation; D009693:Nucleic Acid Hybridization; D011120:Polyomavirus / Q000235:genetics*; D014176:Protein Biosynthesis; D013045:Species Specificity
D004279:DNA, Viral; D004262:DNA Restriction Enzymes