Involvement of local adrenergic receptors in the process of ovulation in gonadotrophin-primed immature rats.
Immature female rats were primed with 4 i.u. PMSG at 08:00 h of Day 26. This results in ovulation in the morning of Day 29. The number of ovulations was counted in terms of newly formed corpora lutea in the morning of Day 30. Various adrenergic drugs were delivered into the ovarian bursa bilaterally in the afternoon of Day 27 to study their effect on ovulation. A methyl cellulose gel solution was used as vehicle to minimize leakage from the bursa. Noradrenaline, terbutaline and 4-aminopyridine significantly enhanced the number of corpora lutea compared to control ovaries injected with gel vehicle alone. The effect of terbutaline was counteracted by propranolol. Phentolamine partly blocked the noradrenaline-induced enhancement and the antagonist alone significantly reduced the number of ovulations. The results indicate that stimulation of alpha-adrenergic receptors (probably via actions in the follicle wall) as well as beta-receptors (influencing steroid-producing cells) may interfere with the ovulation process.
D015761:4-Aminopyridine; D000631:Aminopyridines / Q000494:pharmacology; D000818:Animals; D003338:Corpus Luteum / Q000187:drug effects / Q000502:physiology*; D005260:Female; D006064:Gonadotropins, Equine / Q000494:pharmacology*; D007545:Isoproterenol / Q000494:pharmacology; D009638:Norepinephrine / Q000494:pharmacology; D010060:Ovulation / Q000187:drug effects*; D010062:Ovulation Induction; D010646:Phentolamine / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D011941:Receptors, Adrenergic / Q000502:physiology*; D012741:Sexual Maturation; D013726:Terbutaline / Q000494:pharmacology
D000631:Aminopyridines; D006064:Gonadotropins, Equine; D011941:Receptors, Adrenergic; D015761:4-Aminopyridine; D007545:Isoproterenol; D013726:Terbutaline; D009638:Norepinephrine; D010646:Phentolamine