title: Differences in the properties of bovine brain benzodiazepine receptors in the cerebellum and hippocampus revealed after reduction of disulphide bonds.
abstract: Treatment of bovine brain cell membranes with dithiothreitol decreases the affinity without changing binding capacity for [3H]flunitrazepam in the cerebellum, hippocampus and frontal cortex. Specific binding of beta-[3H]carboline-3-carboxylic acid ethyl ester in the cerebellum and frontal cortex was influenced by dithiothreitol in a similar manner. However, in the hippocampus the binding of this inverse agonist was modified by dithiothreitol in another way: a significant (25%) decrease of binding capacity, or (and) appearance of heterogeneity of binding sites was detected. Sensitivity of benzodiazepine receptors to gamma-aminobutyric acid (GABA) stimulation was not changed by dithiothreitol.
mesh_terms: D000818:Animals; D001923:Brain Chemistry / Q000187:drug effects*; D002243:Carbolines / Q000378:metabolism; D002417:Cattle; D002531:Cerebellum / Q000032:analysis; D002540:Cerebral Cortex / Q000032:analysis; D004229:Dithiothreitol / Q000494:pharmacology*; D005445:Flunitrazepam / Q000378:metabolism; D006624:Hippocampus / Q000032:analysis; D010084:Oxidation-Reduction; D011963:Receptors, GABA-A / Q000032:analysis / Q000187:drug effects* / Q000378:metabolism
chemical_list: D002243:Carbolines; D011963:Receptors, GABA-A; D005445:Flunitrazepam; C024915:beta-carboline-3-carboxylic acid ethyl ester; D004229:Dithiothreitol