Differential regulation of the cyclooxygenase pathway in starch elicited rat peritoneal macrophages by prostaglandin E2, U-44069, a stable endoperoxide analogue and dibutyryl-cyclic AMP.
The basal and carrageenin-stimulated release of thromboxane (TX) B2, the stable product of TXA2, 6-ketoPGF1 alpha, the stable breakdown product of prostacyclin (PGI2) and prostaglandin (PG) E2 from 24 h starch elicited rat peritoneal macrophages was inhibited by dibutyryl-cyclic AMP (db-cAMP). PGE2 also inhibited the release of TXA2 and 6-keto-PGF1 alpha whereas the stable endoperoxide analogue, U-44069, stimulated PGE2 and 6-keto PGF1 alpha release but inhibited TXB2 release. The effects of all three mediators tested were related to an increase of Mø intracellular cAMP content.
D015121:6-Ketoprostaglandin F1 alpha / Q000494:pharmacology; D000818:Animals; D003994:Bucladesine / Q000494:pharmacology*; D015232:Dinoprostone; D066298:In Vitro Techniques; D008264:Macrophages / Q000378:metabolism*; D008297:Male; D011450:Prostaglandin Endoperoxides, Synthetic / Q000494:pharmacology*; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism*; D011458:Prostaglandins E / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D013213:Starch / Q000494:pharmacology*; D013929:Thromboxane B2 / Q000378:metabolism
D011450:Prostaglandin Endoperoxides, Synthetic; D011458:Prostaglandins E; D013929:Thromboxane B2; C028429:U 44069; D015121:6-Ketoprostaglandin F1 alpha; D003994:Bucladesine; D013213:Starch; D011451:Prostaglandin-Endoperoxide Synthases; D015232:Dinoprostone