Inhibition of protein synthesis enhances transformation of primary cells by viral DNA.
Inhibition of protein synthesis by cycloheximide after transfection and subsequent removal of the drug increased the transformation efficiency of primary cells by plasmids containing the left 4.5, 6.7, or 16% of the adenovirus (Ad) genome. The enhancement factor ranged from 2 to as much as 70 depending on the size of the viral DNA fragments used. Addition of cycloheximide before or at the time of transfection inhibited transformation, suggesting that viral protein synthesis is important during the early phase of transformation. Transient expression assays showed that cells treated with cycloheximide post-transfection contained as much as three times the amount of viral RNA transcribed from regions E1A and E1B. Conversion of a rat cell line lacking thymidine kinase activity (TK-) to the TK+ phenotype by a plasmid containing the herpes TK gene was severely inhibited by the drug treatment, suggesting that the enhancement effects of cycloheximide on transformation may be specific for Ad DNA. Cycloheximide treatment also increased the number of transformants induced by a transformation defective E1B mutant of Ad12 (cyt mutant). Plasmid containing only the E1A region of Ad12 transformed primary rat kidney cells with very low efficiency. The inclusion of E1B in the transfecting DNA fragments increased the transformation frequency by more than 400-fold, much higher than that achieved by cycloheximide. Thus, cycloheximide cannot replace E1B functions in transformation efficiency.
D000260:Adenoviruses, Human / Q000187:drug effects / Q000235:genetics*; D002472:Cell Transformation, Viral / Q000187:drug effects*; D003001:Cloning, Molecular; D003513:Cycloheximide / Q000494:pharmacology*; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000235:genetics*; D006801:Humans; D007624:KB Cells / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D014176:Protein Biosynthesis / Q000187:drug effects*; D014158:Transcription, Genetic; D014162:Transfection
D004279:DNA, Viral; D003513:Cycloheximide; D004262:DNA Restriction Enzymes