title: Inflammation induced by bacterial cell wall fragments in the rat air pouch. Comparison of rat strains and measurement of arachidonic acid metabolites.
abstract: Streptococcal cell wall fragments, suspended in phosphate-buffered saline, were injected into a preformed subcutaneous air pouch in rats. The advantage of the air pouch model is the capacity for quantitation of exudative, cellular, and proliferative responses and soluble mediators. Accumulation of pouch fluid containing many leukocytes occurred during the first 3 days. Granulation tissue separable from the surrounding subcutaneous tissue developed by 6 days. Immunofluorescence and immunoperoxidase staining showed the presence of cell walls in inflammatory cells both in pouch fluid and in pouch tissue. Histologic features of this inflammation included an acute exudative phase with a predominantly neutrophil infiltration followed by a chronic phase characterized by fibroblast proliferation, formation of blood vessels, and infiltration with mononuclear cells. The lining of the pouch before injection of cell wall developed morphologic features of synovial membrane, which became more evident during the chronic phase of induced inflammation. Outbred Sprague-Dawley and inbred Lewis rats developed more pouch fluid, cell numbers in the pouch fluid, and granulation tissue than inbred Buffalo rats. The arachidonic acid metabolites, prostaglandin E2 and leukotriene B4, were measured in the pouch fluid, and more of each was produced in the Lewis than in the Buffalo strain. These measurements of inflammation are consistent with the relative susceptibility of these strains to cell-wall-induced arthritis. This model of inflammation can be used in the examination of the regulatory mechanisms of evolving chronic inflammation.
mesh_terms: D000818:Animals; D002473:Cell Wall / Q000276:immunology; D015232:Dinoprostone; D004195:Disease Models, Animal; D007249:Inflammation / Q000276:immunology* / Q000473:pathology; D007975:Leukotriene B4 / Q000032:analysis; D011458:Prostaglandins E / Q000032:analysis; D051381:Rats; D011915:Rats, Inbred BUF; D011917:Rats, Inbred Lew; D013045:Species Specificity; D013297:Streptococcus pyogenes / Q000276:immunology*; D013997:Time Factors
chemical_list: D011458:Prostaglandins E; D007975:Leukotriene B4; D015232:Dinoprostone