Effect of insulin on an insulin receptor of PLC/PRF/5 human hepatoma cell line.
A human hepatoma cell line (PLC/PRF/5) contains several copies of hepatitis B virus (HBV) DNA in integrated form and releases hepatitis B surface antigen (HBsAg) in the form of 22 nm particles in culture medium; studies of the supernatant fluid failed to provide evidence of the morphologically intact virion (Dane particle) or hepatitis B infectivity. In this paper we describe the effect of insulin on cell growth and HBsAg production by these cells; moreover we describe the insulin binding to specific cell membrane receptors. Data in our hands point to a different insulin binding related to different incubation conditions.
D001667:Binding, Competitive; D006528:Carcinoma, Hepatocellular / Q000378:metabolism* / Q000382:microbiology; D002455:Cell Division; D002460:Cell Line; D003470:Culture Media; D006515:Hepatitis B virus; D006801:Humans; D007328:Insulin / Q000494:pharmacology*; D008113:Liver Neoplasms / Q000378:metabolism* / Q000382:microbiology; D011972:Receptor, Insulin / Q000187:drug effects*
D003470:Culture Media; D007328:Insulin; D011972:Receptor, Insulin