[Chemical synthesis, isolation and sequencing of tetradecadeoxyribonucleotides containing the modified bases 5-fluorouracil and 5-methylcytosine].
It is reported on phosphate-triester synthesis, isolation and sequencing of five tetradecadeoxyribonucleotides containing the modified bases 5-fluorouracil and 5-methylcytosine. The modified nucleotides can be exactly determined in the oligonucleotide chain by solid-phase MAXAM/GILBERT-sequencing. By combination of the oligonucleotides new hemi-modified DNA-duplexes were constructed which serve as model compounds for investigating the interaction with the EcoRII restriction and modification enzymes.
D044503:5-Methylcytosine; D001483:Base Sequence; D003596:Cytosine / Q000031:analogs & derivatives; D004262:DNA Restriction Enzymes; D015252:Deoxyribonucleases, Type II Site-Specific; D003854:Deoxyribonucleotides / Q000138:chemical synthesis / Q000302:isolation & purification; D005472:Fluorouracil; D009692:Nucleic Acid Heteroduplexes
D003854:Deoxyribonucleotides; D009692:Nucleic Acid Heteroduplexes; D044503:5-Methylcytosine; D003596:Cytosine; D004262:DNA Restriction Enzymes; C046545:CCWGG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific; D005472:Fluorouracil
Chemische Synthese, Isolierung und Sequenzierung von Tetradecadesoxyribonucleotiden mit den abgewandelten Basen 5-Fluoruracil und 5-Methylcytosin.