title: Effect of herpes simplex virus type 1 on cellular pools of oligosaccharide-lipid.
abstract: Incorporation of [3H]mannose into cellular pools of mannosylphosphoryl dolichol (Man-P-Dol), oligosaccharide-lipid, and glycoprotein was measured and compared in herpes simplex virus type 1 (HSV-1)-infected cells and -uninfected cells. While mannose incorporation into the monosaccharide-dolichol fraction was similar in infected or uninfected Vero cells, incorporation into the oligosaccharide-lipid fraction was markedly reduced in HSV-1-infected cells (64% of control levels). In contrast, mannose incorporation into glycoprotein was significantly increased in virus-infected cells versus uninfected cells (194% of control levels). The kinetics of incorporation into the various fractions was examined and it was determined that there was minimal increase in mannose incorporation into oligosaccharide-lipid after 8 hr postinfection in virus-infected cells. This corresponded to the time at which nonglycosylated precursors of the HSV-1 glycoproteins were first detected in association with the nuclear fraction. These data suggest that there is an accelerated turnover of oligosaccharide-lipid in virus-infected Vero cells which is most likely due to extensive glycoprotein synthesis.
mesh_terms: D000818:Animals; D002460:Cell Line; D002472:Cell Transformation, Viral; D002522:Chlorocebus aethiops; D004287:Dolichol Monophosphate Mannose / Q000096:biosynthesis*; D006017:Glycolipids / Q000096:biosynthesis*; D006023:Glycoproteins / Q000378:metabolism*; D007668:Kidney; D007700:Kinetics; D008358:Mannose / Q000378:metabolism*; D011105:Polyisoprenyl Phosphate Sugars / Q000096:biosynthesis*; D011499:Protein Processing, Post-Translational; D018139:Simplexvirus / Q000235:genetics / Q000378:metabolism*; D014759:Viral Envelope Proteins; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D006017:Glycolipids; D006023:Glycoproteins; D011105:Polyisoprenyl Phosphate Sugars; D014759:Viral Envelope Proteins; D014764:Viral Proteins; C038535:glycoprotein gC, herpes simplex virus type 1; D004287:Dolichol Monophosphate Mannose; D008358:Mannose