title: Virus production and surface marker expression in human lymphocytes immortalized following dual infection with human T-cell leukemia virus and Epstein-Barr virus.
abstract: By co-cultivating peripheral blood lymphocytes from normal male donors with cells from a line designated Lma-66VP, established from a female donor and simultaneously producing both HTLV-I and EBV, 2 continuous culture lines were obtained. Normal male lymphocytes were considered to be immortalized by co-cultivation because they were of male karyotype and lacked the 3q+ chromosome that was observed in all Lma-66VP cells. These immortalized cultures were designated Co-culture 1 and Co-culture 2. After prolonged cultivation, chromosome abnormalities characteristic of each co-culture line were observed in all cells examined, indicating their clonal origin. Immortalized cells initially produced both HTLV-I and EBV. Although production of EBV was not seen in the co-culture lines after prolonged cultivation when all cells examined had the characteristic chromosomal abnormality indicating their clonality, EBNA+ cells persisted. Thus, clonal cells were doubly infected by HTLV-I and EBV. While the majority of clonal cells in Lma-66VP, Co-culture 1, and Co-culture 2 lines expressed a surface marker of B-cells, small numbers of cells expressed a T-cell surface marker. These findings demonstrate that human lymphocytes immortalized following dual infection by HTLV-I and EBV exhibit chromosome rearrangement, resulting in expression of surface markers inconsistent with their differentiation lineage.
mesh_terms: D000328:Adult; D000911:Antibodies, Monoclonal / Q000276:immunology; D000944:Antigens, Differentiation, B-Lymphocyte; D000954:Antigens, Surface / Q000032:analysis; D000956:Antigens, Viral / Q000032:analysis; D002460:Cell Line; D002869:Chromosome Aberrations; D017977:Deltaretrovirus / Q000254:growth & development* / Q000276:immunology; D019309:Epstein-Barr Virus Nuclear Antigens; D004854:Herpesvirus 4, Human / Q000254:growth & development* / Q000276:immunology; D006801:Humans; D007136:Immunoglobulins / Q000096:biosynthesis; D007621:Karyotyping; D008214:Lymphocytes / Q000276:immunology / Q000382:microbiology* / Q000648:ultrastructure; D008297:Male
chemical_list: D000911:Antibodies, Monoclonal; D000944:Antigens, Differentiation, B-Lymphocyte; D000954:Antigens, Surface; D000956:Antigens, Viral; D019309:Epstein-Barr Virus Nuclear Antigens; D007136:Immunoglobulins