Interaction between polymorphonuclear leukocytes and varicella-zoster virus-infected cells.
The addition of polymorphonuclear leukocytes (PMNL) to human fibroblasts infected with varicella-zoster virus (VZV) resulted in a reduced virus yield. The reduction was greater when antibodies specific for VZV were added to the system. Addition of VZV-specific antibodies without PMNL also reduced virus yield, but a 10-fold greater concentration of antibodies was required to effect the same reduction. PMNL adhered to and formed rosettes around VZV-infected cells. By electron microscopy, it was possible to observe activation of PMNL incubated with VZV-infected fibroblasts. Activated PMNL extended cytoplasmic projections toward virions and had vacuoles containing virions in various stages of digestion.
D002478:Cells, Cultured; D005347:Fibroblasts; D014645:Herpesvirus 3, Human / Q000254:growth & development / Q000276:immunology*; D006801:Humans; D008854:Microscopy, Electron; D009504:Neutrophils / Q000276:immunology* / Q000648:ultrastructure; D010587:Phagocytosis; D012397:Rosette Formation; D010948:Viral Plaque Assay