Genomic localization of hepatitis B virus in a human hepatoma cell line.
The integration of hepatitis B viral sequences in the human hepatoma Alexander cell line has been investigated after fractionation of the cell line DNA by centrifugation in a Cs2SO4/BAMD (3,6-(bis-acetato mercurimethyl) dioxane) density gradient. Eight out of nine integrated viral sequences were localized in DNA component H3, which only represents 4% of the human genome and matches the base composition of HBV sequences. These results indicate a targeting and/or a higher stability of the latter in a specific, small compartment of the host genome.
D006528:Carcinoma, Hepatocellular / Q000382:microbiology*; D002460:Cell Line; D004262:DNA Restriction Enzymes; D004273:DNA, Neoplasm / Q000032:analysis*; D004279:DNA, Viral / Q000032:analysis*; D006515:Hepatitis B virus / Q000235:genetics*; D006801:Humans; D008113:Liver Neoplasms / Q000382:microbiology*; D009693:Nucleic Acid Hybridization
D004273:DNA, Neoplasm; D004279:DNA, Viral; D004262:DNA Restriction Enzymes