title: HVJ (Sendai virus) stimulates release of interferon from leukocytes used once for interferon production.
abstract: Leukocytes, subjected once to interferon (IFN) induction by HVJ (Sendai virus), were studied for their capability to produce IFN after a second similar stimulus. Substantial amounts of IFN (about 30 000 IU/ml) were recovered. Experiments using cycloheximide or actinomycin D and kinetic studies showed that this IFN originated mainly in IFN which resided within the cell as a result of the first induction and was released after the second stimulation. Increasing amounts of HVJ used for the second stimulus resulted in proportionally increased yields of IFN, reaching a plateau at the same dose of HVJ (1000 HAU/ml) as that which gave optimal yields after the first stimulation. Evidence is presented that the capacity of HVJ to trigger the production of a second IFN harvest is closely associated with its infectivity.
mesh_terms: D002478:Cells, Cultured; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D006801:Humans; D007370:Interferon Type I / Q000096:biosynthesis / Q000378:metabolism*; D007371:Interferon-gamma / Q000096:biosynthesis / Q000378:metabolism; D007962:Leukocytes / Q000378:metabolism*; D010222:Parainfluenza Virus 1, Human / Q000502:physiology*
chemical_list: D007370:Interferon Type I; D003609:Dactinomycin; D007371:Interferon-gamma; D003513:Cycloheximide