[Effect of thyroid hormone receptors in normal and cancerous cells on the ionic conductivity of bilayer phospholipid membranes].
The effect of thyroid hormones receptors isolated from normal and cancer cells on bilayer phospholipid membranes (BPhLM) conductivity, has been studied. The receptor isolated from normal cells in complex X with the hormone selectively induces H+-conductivity of BPhLM generating transmembrane potential equal to 42 mV on the membrane at pH gradient equal to 1. In the presence of K+, Na+, Ca+, Mn2+, Sr2+, Mg2+ the changes of BPhLM are not observed. Neither hormones (T3, T4) nor receptor in free position affect the BPhLM conductivity. Thyroid hormone receptor isolated from mamalignantly transformed cells in a complex with T3 or T4 increases the BPhLM permeability for Ca2+. The transmembrane potential measured at 10fold Ca2+ ion concentration is equal to 16 mV. In the presence of H+, K+, Na+, Mn2+, Sr2+, Mg2+, Ba2+, the resistance of BPhLM doesn't change.
D002463:Cell Membrane Permeability / Q000187:drug effects*; D004622:Embryo, Mammalian; D006367:HeLa Cells; D006801:Humans; D007477:Ions; D008051:Lipid Bilayers / Q000378:metabolism*; D008563:Membrane Lipids / Q000378:metabolism*; D008564:Membrane Potentials / Q000187:drug effects; D009369:Neoplasms / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000302:isolation & purification / Q000502:physiology*; D013963:Thyroid Hormones / Q000502:physiology*
D007477:Ions; D008051:Lipid Bilayers; D008563:Membrane Lipids; D010743:Phospholipids; D011956:Receptors, Cell Surface; D013963:Thyroid Hormones
Vliianie retseptorov tireoidnykh gormonov normal'nykh i rakovykh kletok na ionnuiu provodimost' bisloĭnykh fosfolipidnykh membran.