Mapping of the varicella zoster virus deoxypyrimidine kinase gene and preliminary identification of its transcript.
The varicella-zoster virus (VZV) deoxypyrimidine kinase (dPK) gene was mapped by transfection of cloned viral DNA fragments into thymidine kinase-deficient mouse L (LTK-) cells and subsequent biochemical transformation of these cells to the LTK+ phenotype. Such transforming activity was limited to the BamHI-H and EcoRI-D fragments of the VZV genome, which overlap by 2.2 kb between map units 0.50 and 0.52. Biochemically transformed cells were shown to contain a high copy number of viral DNA sequences that had integrated into the cellular DNA. Extracts of these cells showed a higher level of dPK activity than did extracts of parental LTK- cells. With the use of Northern hybridization analysis of transformed and VZV-infected cell RNAs, it was possible to tentatively assign a 1.8-kb transcript to the VZV dPK.
D000818:Animals; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D003842:Deoxycytidine Kinase / Q000235:genetics / Q000378:metabolism; D015245:Deoxyribonuclease BamHI; D015246:Deoxyribonuclease EcoRI; D005814:Genes, Viral; D014645:Herpesvirus 3, Human / Q000201:enzymology / Q000235:genetics*; D007739:L Cells; D051379:Mice; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*; D013937:Thymidine Kinase / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic; D014162:Transfection
D012333:RNA, Messenger; D012367:RNA, Viral; D013937:Thymidine Kinase; D003842:Deoxycytidine Kinase; D004262:DNA Restriction Enzymes; D015245:Deoxyribonuclease BamHI; D015246:Deoxyribonuclease EcoRI