[Molecular cloning of MuLV proviruses integrated into the genome of mouse erythroleukemia cells. I. Characteristics of endogenous proviruses].
The library of genes was obtained from erythroleukemic AKR cells (C-1), that were maintained as suspension culture. Thirty four clones that had homology with 60-70S RNA of Rauscher Leukemia virus (RLV) were separated from this library. The restriction mapping was carried out with 14 clones, that contained most extensive proviral sequences. One clone (107) contains proviral sequences that are derived from one of the components of the RLV complex. The other 13 clones contain sequences of endogenous xenotropic viruses. The endogenous retroviral sequences obtained differ in restrictive maps from proviruses of ecotropic and xenotropic infectious endogenous MuLV and, apparently, might be attributed as non-inducible infectious xenotropic MuLV of class III. Some of the cloned retroviral sequences had symmetrical structure, that is typical for integrated proviruses, i. e. these sequences were separated from flanking cellular ones by long terminal repeats. All investigated retroviral sequences are deletion mutants of MuLV proviruses. It was shown that the inner regions of proviruses diverged more than the long terminal repeats. The expression of the main inner MuLV polypeptide (p30) was detected in NIH 3T3 cells, transfected with DNA of some clones.
D000818:Animals; D002460:Cell Line; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004262:DNA Restriction Enzymes; D004273:DNA, Neoplasm / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics*; D009052:Leukemia Virus, Murine / Q000235:genetics*; D004915:Leukemia, Erythroblastic, Acute / Q000235:genetics / Q000382:microbiology*; D051379:Mice; D008806:Mice, Inbred AKR; D010957:Plasmids; D011925:Rauscher Virus / Q000235:genetics; D011995:Recombination, Genetic
D004273:DNA, Neoplasm; D004279:DNA, Viral; D004247:DNA; D004262:DNA Restriction Enzymes
Molekuliarnoe klonirovanie provirusov MuLV, integrirovannykh v genom myshinykh éritroleĭkoznykh kletok. I. Kharakteristika provirusov éndogennogo proiskhozhdeniia.