Single cell cloning of Epstein-Barr virus transformed cells in 20-microliter hanging drops.
A simple method for cloning EBV transformants from single cells in 20-microliter hanging drops is described. The hanging-drop method allows direct verification prior to the addition of irradiated feeder cells that clones will be established from single cells. The average efficiency of cloning from wells containing a single transformed cell was 74%, ranging from 60 to 95% in eight separate experiments.
D001402:B-Lymphocytes / Q000166:cytology*; D002460:Cell Line; D002469:Cell Separation; D002472:Cell Transformation, Viral; D002999:Clone Cells / Q000166:cytology*; D046508:Culture Techniques / Q000295:instrumentation / Q000379:methods*; D004854:Herpesvirus 4, Human / Q000502:physiology*; D006801:Humans