Biogenesis of liver delta-aminolevulinate synthase. The role of cAMP in the induction.
In primary cultures of chick embryo hepatocytes pulse labeled with [35S]methionine, immunochemical analyses indicated that adenosine 3':5'-cyclic monophosphate (cAMP) did not affect either the rate of production or the maturation of delta-aminolevulinate synthase (ALA synthase). In addition, allylisopropylacetamide caused a slight drop in intracellular cAMP while testosterone caused the levels of cAMP to rise to 260% of the basal levels measured in hepatocytes in culture. Thus the results of this study did not indicate a direct short-term role for cAMP in the regulation of production of ALA synthase.
D000624:5-Aminolevulinate Synthetase / Q000096:biosynthesis*; D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D000242:Cyclic AMP / Q000032:analysis / Q000502:physiology*; D004790:Enzyme Induction; D008099:Liver / Q000201:enzymology*
D000242:Cyclic AMP; D000624:5-Aminolevulinate Synthetase