title: Analysis of the sheep MHC using HLA class I, II, and C4 cDNA probes.
abstract: Four cDNA probes for the human major histocompatibility complex (MHC) were used to investigate the sheep MHC, in conjunction with serological typing for ovine lymphocyte antigen (OLA). Lymphocytes from a family (two parents and five offspring) of Romanov sheep were subjected to genomic DNA digestion by the restriction endonuclease Eco RI, followed by gel electrophoresis. A single Southern blot representing all seven individuals was then consecutively hybridized with the class I, alpha-DC, beta-DR, and C4 probes, which were originally designed to identify HLA class I, class II (DC and DR), and C4 products, respectively. Using each of the three class I/class II probes, several bands showing DNA polymorphism were detected. The segregation of these bands in the five offspring exactly paralleled the OLA haplotype segregation established by serological typing. A further eight individuals carrying haplotypes which were phenotypically identical to those in the above-mentioned family showed bands in the corresponding positions when tested with the same three probes. Using the C4 probe, no polymorphism was detected in these fifteen individuals.
mesh_terms: D000818:Animals; D002874:Chromosome Mapping; D003181:Complement C4 / Q000235:genetics*; D004247:DNA / Q000235:genetics; D004262:DNA Restriction Enzymes; D008040:Genetic Linkage; D005838:Genotype; D006649:Histocompatibility Antigens / Q000235:genetics*; D000949:Histocompatibility Antigens Class II / Q000235:genetics*; D006801:Humans; D008285:Major Histocompatibility Complex; D011110:Polymorphism, Genetic; D012689:Sequence Homology, Nucleic Acid; D012756:Sheep / Q000235:genetics / Q000276:immunology*
chemical_list: D003181:Complement C4; D006649:Histocompatibility Antigens; D000949:Histocompatibility Antigens Class II; D004247:DNA; D004262:DNA Restriction Enzymes