[Adaptation to the cell line PLC/PRF/5 of hepatitis A virus released in the cell culture medium].
The propagation of hepatitis A virus (HAV), CF53 strain, released without any cytopathic effect into the PLC/PRF/5 cells supernatant, was studied in the course of six serial passages (6th to 11th). The decrease (from 5 to 1 week) of incubation time required to detect HAV, by RIA, in culture supernatant, the increase in Hepatitis A antigen (from 777 to 10,038 c.p.m./50 microliter) and infectivity titre (from 10(3.0) TCID 50/ml to 10(4.5) TCID 50/ml) were consistent with the adaptation of this virus to the cell line PLC/PRF/5.
D006528:Carcinoma, Hepatocellular; D002460:Cell Line; D003470:Culture Media; D004261:DNA Replication; D006507:Hepatovirus / Q000254:growth & development*; D006801:Humans; D007700:Kinetics; D008113:Liver Neoplasms; D013997:Time Factors; D014779:Virus Replication
D003470:Culture Media
Adaptation à la lignée cellulaire PLC/PRF/5 du virus de l'hépatite A libéré dans le surnageant de culture.