SV40 vector with early gene replacement efficient in transducing exogenous DNA into mammalian cells.
An early replacement SV40 vector, SV40-Mu beta, was constructed by replacing part of the early genes of the virus with mouse interferon-beta (IFN-beta) cDNA. Upon transfection of COS-7 cells with this DNA, transducing viral particles were produced, which could infect various cells and cause efficient production of mouse IFN-beta. The viral stock contained no detectable wild-type SV40. The IFN production after the virus infection was very high in monkey kidney cells, but less so in human epithelial cells, and low in mouse, pig, hamster cells and in human lymphocytes. The efficiency of introduction of the DNA to monkey kidney cells was compared with that by the calcium phosphate precipitation method, and the viral vector was found to be more efficient by a factor of several tens to hundreds.
D000818:Animals; D002130:Calcium Phosphates; D011232:Chemical Precipitation; D004247:DNA / Q000235:genetics; D005814:Genes, Viral; D005818:Genetic Engineering / Q000379:methods; D005822:Genetic Vectors; D000882:Haplorhini; D007370:Interferon Type I / Q000096:biosynthesis / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis*; D013539:Simian virus 40 / Q000235:genetics*; D014161:Transduction, Genetic
D002130:Calcium Phosphates; D007370:Interferon Type I; D011994:Recombinant Proteins; C485828:alpha-tricalcium phosphate; C485830:tetracalcium phosphate; C485838:calcium phosphate, monobasic, anhydrous; D004247:DNA; C020243:calcium phosphate; C485829:calcium phosphate, dibasic, anhydrous