Protein kinase C promotes the phosphorylation of immunoprecipitated middle T antigen from polyoma-transformed cells.
Stimulation of protein kinase C in polyoma virus-transformed cells increased the phosphorylation of tyrosine residues of the viral middle T (mT) antigen in mT:pp60c-src complexes precipitated by anti-mT antibodies. This increase might have been due to a stimulation of the complex's pp60c-src tyrosine kinase activity or to an increased ability of the mT protein to be phosphorylated by pp60c-src. These observations suggest that cellular protein kinase C might control the ability of polyoma virus to transform its host cell.
D000818:Animals; D000952:Antigens, Polyomavirus Transforming; D000957:Antigens, Viral, Tumor / Q000378:metabolism*; D000001:Calcimycin / Q000494:pharmacology; D002472:Cell Transformation, Viral; D003720:Densitometry; D051379:Mice; D008970:Molecular Weight; D015688:Oncogene Protein pp60(v-src); D009856:Oncogene Proteins, Viral / Q000378:metabolism*; D010766:Phosphorylation; D011120:Polyomavirus; D011493:Protein Kinase C / Q000378:metabolism*; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D051381:Rats; D012191:Retroviridae Proteins / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013912:Threonine / Q000378:metabolism; D014443:Tyrosine / Q000378:metabolism; D014638:Vanadates; D014639:Vanadium / Q000494:pharmacology
D000952:Antigens, Polyomavirus Transforming; D000957:Antigens, Viral, Tumor; D009856:Oncogene Proteins, Viral; D012191:Retroviridae Proteins; D014639:Vanadium; D013912:Threonine; D000001:Calcimycin; D014638:Vanadates; D014443:Tyrosine; D011505:Protein-Tyrosine Kinases; D015688:Oncogene Protein pp60(v-src); D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate