The effect of 1-cyclohexyl-3-(2-morpholinoethyl)carbodiimide on the rat liver microsomal glucose-6-phosphatase system.
The effect of 1-cyclohexyl-3-(2-morpholinoethyl)carbodiimide (CMC) on the reactions catalyzed by the glucose-6-phosphatase system of rat liver microsomes was studied. Modification of the intact microsomes by CMC leads to the inhibition of the glucose-6-phosphatase, pyrophosphate:glucose and carbamoyl-phosphate : glucose phosphotransferase activities of the system. The activities are restored by the disruption of the microsomal permeability barrier. The mannose-6-phosphate, pyrophosphate, and carbamoyl-phosphate phosphohydrolase activities of the intact as well as the disrupted microsomes were not affected by CMC. It follows from the results obtained that CMC inactivates the microsomal glucose-6-phosphate translocase, the inactivation is a result of the modification of a single sulfhydryl or amino group of the translocase; pyrophosphate, carbamoyl phosphate and inorganic phosphate are transported across the microsomal membrane without participation of the glucose-6-phosphate translocase; pyrophosphate and carbamoyl phosphate may act as the phosphate donors in the glucose phosphorylation reactions in vivo.
D000818:Animals; D017920:Antiporters; D001692:Biological Transport / Q000187:drug effects; D003028:CME-Carbodiimide / Q000031:analogs & derivatives / Q000494:pharmacology*; D002234:Carbodiimides / Q000494:pharmacology*; D002384:Catalysis; D005952:Glucose-6-Phosphatase / Q000037:antagonists & inhibitors*; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology*; D009004:Monosaccharide Transport Proteins; D010766:Phosphorylation; D010770:Phosphotransferases / Q000037:antagonists & inhibitors; D051381:Rats
D017920:Antiporters; D002234:Carbodiimides; D009004:Monosaccharide Transport Proteins; C503533:Slc37a4 protein, rat; C111261:glucose 6-phosphate(transporter); D003028:CME-Carbodiimide; D010770:Phosphotransferases; D005952:Glucose-6-Phosphatase