Modulation of the number of muscarinic receptors in mouse neuroblastoma cells by soman.
Long-term preincubation at 37 degrees of mouse neuroblastoma cells (clones NS-20 and N1E-115) with soman, a potent and irreversible cholinesterase inhibitor, resulted in a significant decrease in the number of [3H]N-methylscopolamine binding sites and in the inhibition of carbamylcholine-induced cyclic GMP formation. The disappearance of surface muscarinic receptors and the desensitization of the receptor-mediated response seem to occur via accumulation of acetylcholine in the culture medium. The significance of these findings is discussed.
D000109:Acetylcholine / Q000032:analysis; D000818:Animals; D002478:Cells, Cultured; D006152:Cyclic GMP / Q000096:biosynthesis; D051379:Mice; D019832:N-Methylscopolamine; D009447:Neuroblastoma / Q000032:analysis; D011976:Receptors, Muscarinic / Q000032:analysis / Q000187:drug effects*; D012602:Scopolamine Derivatives / Q000378:metabolism; D012999:Soman / Q000494:pharmacology*
D011976:Receptors, Muscarinic; D012602:Scopolamine Derivatives; D012999:Soman; D006152:Cyclic GMP; D000109:Acetylcholine; D019832:N-Methylscopolamine