title: A perfused rabbit retina preparation suitable for pharmacological studies.
abstract: We present a method for maintaining the isolated retina-eyecup of the rabbit in a manner which permits the introduction of pharmacological agents at a controlled concentration. As judged by physiological criteria, the retina is well maintained under these conditions. The stability of the preparation is excellent; intracellular or extracellular recordings from single cells can be maintained through multiple solution changes. By using Co2+ to block synaptic transmission, we can distinguish direct from transsynaptic effects. Use of this preparation should facilitate the investigation of neurotransmission in the mammalian retina.
mesh_terms: D000818:Animals; D003035:Cobalt / Q000494:pharmacology; D009474:Neurons / Q000187:drug effects; D010477:Perfusion; D011817:Rabbits; D012160:Retina / Q000187:drug effects*; D012165:Retinal Ganglion Cells / Q000187:drug effects; D013569:Synapses / Q000187:drug effects; D009435:Synaptic Transmission / Q000187:drug effects
chemical_list: D003035:Cobalt