Enzyme-linked immunosorbent assay for the detection of bovine papillomavirus.
An enzyme-linked immunosorbent assay has been developed to detect and quantitate bovine papillomavirus in partially purified and in purified viral preparations, using rabbit antiserum against group-specific papillomavirus structural antigens and alkaline phosphatase-labeled affinity purified goat antibody to rabbit immunoglobulin G. Viral detection correlated well with negative-stain electron microscopy of the various preparations and peroxidase-antiperoxidase staining of paraffin sections of the original fibropapillomas. The technique is rapidly done and will detect minute amounts of viral protein (1 ng/ml).
D000818:Animals; D052688:Bovine papillomavirus 1 / Q000302:isolation & purification*; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D004797:Enzyme-Linked Immunosorbent Assay; D006965:Hyperplasia / Q000662:veterinary; D007124:Immunoenzyme Techniques; D008854:Microscopy, Electron; D027383:Papillomaviridae / Q000302:isolation & purification*; D012867:Skin / Q000382:microbiology / Q000473:pathology; D014412:Tumor Virus Infections / Q000382:microbiology / Q000662:veterinary*