In vitro binding of platinum compounds to human transferrin.
The binding of the antineoplastic drug cis-dichlorodiammineplatinum II (CDDP) and a related complex, potassium tetrachloroplatinite (K2PtCl4), to human transferrin in buffered anion solutions was studied over a period of two weeks. Separation of bound and free platinum was accomplished by gel chromatography with platinum analysis by atomic absorption spectrophotometry. The results demonstrated that K2PtCl4 was bound to a greater degree than CDDP in this system with 3-5 and 1-2 platinum atoms respectively, bound per transferrin molecule. However, it appears this interaction, which may involve the specific cation binding sites, does not play a significant role in the pharmacology of CDDP in vivo.
D001639:Bicarbonates; D002712:Chlorides / Q000378:metabolism*; D002945:Cisplatin / Q000378:metabolism*; D002951:Citrates; D019343:Citric Acid; D006801:Humans; D066298:In Vitro Techniques; D009571:Nitrilotriacetic Acid; D010984:Platinum / Q000378:metabolism*; D017671:Platinum Compounds; D012964:Sodium; D017693:Sodium Bicarbonate; D014168:Transferrin / Q000378:metabolism*
D001639:Bicarbonates; D002712:Chlorides; D002951:Citrates; D017671:Platinum Compounds; D014168:Transferrin; C036919:tetrachloroplatinate; D019343:Citric Acid; D010984:Platinum; D017693:Sodium Bicarbonate; D012964:Sodium; D009571:Nitrilotriacetic Acid; D002945:Cisplatin