Astrocytes have both M1 and M2 muscarinic receptor subtypes.
In astrocytes, carbachol evoked the turnover of membrane inositol phospholipids prelabelled with [3H]inositol, as revealed by [3H]inositol phosphate accumulation in the presence of 5 mM lithium. This effect was blocked by atropine and by pirenzepine (IC50 2.2 nM and 56 nM, respectively). Carbachol partially attenuated the isoproterenol-stimulated cyclic adenosine 3',5'-monophosphate production in astrocytes by a direct effect on adenylate cyclase, an effect blocked by atropine and pirenzepine. These results suggest that astrocytes express muscarinic receptor subtypes.
D000818:Animals; D001253:Astrocytes / Q000032:analysis* / Q000378:metabolism; D001285:Atropine / Q000494:pharmacology; D001570:Benzodiazepinones / Q000494:pharmacology; D002217:Carbachol / Q000494:pharmacology; D000242:Cyclic AMP / Q000096:biosynthesis*; D007294:Inositol / Q000378:metabolism*; D007545:Isoproterenol / Q000494:pharmacology; D010743:Phospholipids / Q000378:metabolism*; D010890:Pirenzepine; D011433:Propranolol / Q000494:pharmacology; D051381:Rats; D011976:Receptors, Muscarinic / Q000032:analysis*
D001570:Benzodiazepinones; D010743:Phospholipids; D011976:Receptors, Muscarinic; D010890:Pirenzepine; D007294:Inositol; D001285:Atropine; D002217:Carbachol; D011433:Propranolol; D000242:Cyclic AMP; D007545:Isoproterenol