[Reactivity of two monoclonal antibodies (Troma 1 and CAM 5.2) on tissue sections--as a histological trophoblast marker in normal pregnancy and trophoblastic disease].
In normal and molar pregnancy, non-villous trophoblasts which exist in the placental bed are thought to be difficult to be distinguished morphologically from the surrounding maternal cells. We examined the reactivities of two monoclonal antibodies (Troma 1 and CAM 5.2) by an immunoperoxidase technique and analyzed their usefulness as histological trophoblast markers. Materials were taken from 41 uteri of normal pregnancy, 7 uteri of hydatidiform mole, 2 uterine gestational choriocarcinoma, 1 tubal tissue of non-gestational choriocarcinoma and 5 non-gestational uteri. The reactivity of Troma 1 was observed on frozen sections and paraffin sections were employed in the analysis of CAM 5.2. The reactivities of these antibodies were the same. In nongestational tissue, they reacted with endometrial gland epithelia and endometrial covering epithelia. In placental bed of normal pregnancy, they reacted with villous and non-villous trophoblasts, besides endometrial gland epithelia. In molar sections, they reacted with gland epithelia, villous trophoblasts and also non-villous trophoblasts which proliferated towards maternal tissue. In choriocarcinoma, they reacted with carcinoma cells specifically. Since endometrial gland epithelia are easily identified histologically, it was concluded that these antibodies could work as histological trophoblast markers in normal pregnancy and trophoblastic disease.
D000911:Antibodies, Monoclonal / Q000276:immunology*; D002822:Choriocarcinoma / Q000276:immunology; D005260:Female; D006801:Humans; D006828:Hydatidiform Mole / Q000276:immunology; D011247:Pregnancy; D014328:Trophoblastic Neoplasms / Q000276:immunology*; D014327:Trophoblasts / Q000276:immunology*; D014594:Uterine Neoplasms / Q000276:immunology*
D000911:Antibodies, Monoclonal