Induction of DNA breakage and suppression of DNA synthesis by the OH radical generated in a Fenton-like reaction.
The effect of the OH radical, generated in a Fenton-like reaction, on DNA structure and function was studied in a monkey kidney cell line (Vero). DNA single strand breaks were detected following exposure to 10- 100 microM concentrations of H2O2 on ice. These breaks were repaired very rapidly, and addition of the poly (ADP-ribose) transferase inhibitor, 3-aminobenzamide, resulted in an accumulation of breaks. DNA synthesis was inhibited at concentrations as high as 1- 30 mM, this effect also being reversible in approximately 60 min. 3-aminobenzamide did not affect the rate of DNA synthesis inhibition by H2O2.
D000818:Animals; D001549:Benzamides / Q000494:pharmacology; D002460:Cell Line; D002522:Chlorocebus aethiops; D004247:DNA / Q000378:metabolism*; D004260:DNA Repair; D004261:DNA Replication / Q000187:drug effects; D005609:Free Radicals; D006861:Hydrogen Peroxide / Q000494:pharmacology; D006878:Hydroxides / Q000378:metabolism*; D017665:Hydroxyl Radical; D007668:Kidney; D007700:Kinetics
D001549:Benzamides; D005609:Free Radicals; D006878:Hydroxides; D017665:Hydroxyl Radical; C025160:3-aminobenzamide; D004247:DNA; D006861:Hydrogen Peroxide