Cloning in Streptococcus lactis of plasmid-mediated UV resistance and effect on prophage stability.
Plasmid pIL7 (33 kilobases) from Streptococcus lactis enhances UV resistance and prophage stability. A 5.4-kilobase pIL7 fragment carrying genes coding for both characters was cloned into S. lactis, using plasmid pHV1301 as the cloning vector. The recombinant plasmid was subsequently transferred to three other S. lactis strains by transformation or protoplast fusion. Cloned genes were expressed in all tested strains.
D001412:Bacillus subtilis / Q000235:genetics; D001435:Bacteriophages / Q000235:genetics* / Q000502:physiology; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D005822:Genetic Vectors; D005838:Genotype; D013294:Lactococcus lactis / Q000235:genetics / Q000528:radiation effects*; D010641:Phenotype; D010957:Plasmids; D014466:Ultraviolet Rays
D004262:DNA Restriction Enzymes