[Insertion of the cos-site into DNA of phage M13 and its packing in proteins of phage lambda].
The cos-site of lambda phage from pHC79 cosmide is transferred to DNA from M13 mp18 phage. The recombinant DNA thus obtained (MC18) is efficiently packaged into lambda proteins in vitro. The BamHI-HindIII fragment of pGP588 (a pBR322 derivatives containing fragment of human DNA) is subcloned into MC18. Although this pGP588 fragment contains numerous Alu repeats, no essential rearrangements of the insert were revealed. The efficiency infection by recombinant DNA packaged with lambda proteins is about 1 X 10(5) pfu/microgram DNA, whereas in the similar conditions the efficiency of lambda EMBL3A was 1 X 10(6) pfu/microgram. It is assumed that the MC vectors might be suitable for cloning and sequencing large fragments either with cohesive or blunt ends. It opens also the way to construct genomic libraries in single-stranded phages.
D010582:Bacteriophage lambda / Q000235:genetics*; D003090:Coliphages / Q000235:genetics*; D003360:Cosmids; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics*; D009690:Nucleic Acid Conformation; D011487:Protein Conformation; D014764:Viral Proteins / Q000032:analysis / Q000235:genetics*
D004274:DNA, Recombinant; D004279:DNA, Viral; D014764:Viral Proteins; D004262:DNA Restriction Enzymes
Vvedenie cos-uchastka v DNK faga M13 i ee upakovka v belki faga lambda.