title: Paramecium mitochondrial DNA sequences and RNA transcripts for cytochrome oxidase subunit I, URF1, and three ORFs adjacent to the replication origin.
abstract: A 2-kb region adjacent to the replication origin (ori) and a 3-kb region located between the small and large ribosomal RNAs of Paramecium mitochondrial (mt) DNA have been sequenced and the locations of their transcripts determined. The ori segment contains four transcripts, some of which are overlapping, which encode a known protein and two other open reading frames. The other segment encodes, on separate transcripts, the cytochrome c oxidase subunit one gene (COI) and the URF1 gene (ND1) common to most mt genomes. All these genes have the same orientation and do not contain introns. The COI gene is the most divergent of those known and has an internal 108 amino acid 'insert' not found in COI genes from other organisms. With these data it is possible to define a probable Paramecium mt genetic code. With the exception that TGA codes for tryptophan and the use of different start codons, Paramecium mtDNA appears to follow the universal code. GTA possibly can be used as a start codon.
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004261:DNA Replication; D004272:DNA, Mitochondrial / Q000235:genetics*; D003576:Electron Transport Complex IV / Q000235:genetics*; D046911:Macromolecular Substances; D009693:Nucleic Acid Hybridization; D010247:Paramecium / Q000201:enzymology / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D000077278:RNA, Mitochondrial; D014158:Transcription, Genetic
chemical_list: D004272:DNA, Mitochondrial; D046911:Macromolecular Substances; D012333:RNA, Messenger; D000077278:RNA, Mitochondrial; C029305:mitochondrial messenger RNA; D003576:Electron Transport Complex IV