DNA replication in vitro erases a Xenopus 5S RNA gene transcription complex.
Replication of the Xenopus laevis 5S RNA gene in vitro is unimpeded by the presence of a complete transcription complex assembled on the internal control region of the gene. The transcription complex is disrupted by passage of the replication fork, specific transcription factors are displaced, and the daughter 5S RNA genes are inactivated. We have been unable to demonstrate that any "memory" of the preexisting transcription complex is transmitted to the daughter DNA duplexes following replication.
D000818:Animals; D002467:Cell Nucleus / Q000502:physiology; D002474:Cell-Free System; D003593:Cytoplasm / Q000502:physiology; D004261:DNA Replication; D003850:Deoxyribonuclease I; D005786:Gene Expression Regulation; D006367:HeLa Cells; D006801:Humans; D012335:RNA, Ribosomal / Q000235:genetics*; D013539:Simian virus 40 / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics; D014158:Transcription, Genetic; D014982:Xenopus laevis
D012335:RNA, Ribosomal; D014157:Transcription Factors; D003850:Deoxyribonuclease I