Suppression of a nonsense mutation in mammalian cells in vivo by the aminoglycoside antibiotics G-418 and paromomycin.
Aminoglycoside antibiotics in Escherichia coli and yeast can cause ribosomes to read through stop codons during translation. This can result in the phenotypic suppression of nonsense mutations. We show here for the first time that the aminoglycosides G-418 and paromomycin have similar effects in monkey (COS-7) cells in vivo. Suppression of an amber mutation (TAG) by aminoglycosides can restore the activity of a mutant gene transfected into COS-7 cells to almost 20% of wild type levels.
D000123:Acetyltransferases / Q000235:genetics; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D015500:Chloramphenicol O-Acetyltransferase; D002522:Chlorocebus aethiops; D004262:DNA Restriction Enzymes; D005839:Gentamicins / Q000494:pharmacology*; D007668:Kidney; D007700:Kinetics; D009154:Mutation; D010303:Paromomycin / Q000494:pharmacology*; D010957:Plasmids; D013489:Suppression, Genetic / Q000187:drug effects*; D014162:Transfection
D000900:Anti-Bacterial Agents; D005839:Gentamicins; D010303:Paromomycin; C010680:antibiotic G 418; D000123:Acetyltransferases; D015500:Chloramphenicol O-Acetyltransferase; D004262:DNA Restriction Enzymes