[Effect of ouabain on the Na+, K+-ATPase function and multiplication of transformed cells in culture].
A study was made of the ouabain effect (10(-3] on cell proliferation and the dependence of ATP hydrolysis on Na/K-concentration in homogenates of mouse hepatoma (XXIIa) and of L-cells, both sensitive and resistant to etidium bromide. Na+, K+-ATPase activity was found in homogenates of cells from sparse cultures in the presence of ouabain, the activity being stimulated by the Na/K-ratio pecular for the maximum enzymatic activity in cells from the dense cultures. The effect of ouabain on the cell proliferation is similar to the effect of transition of sparse cultures to dense ones.
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002999:Clone Cells / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D004996:Ethidium / Q000494:pharmacology; D006868:Hydrolysis; D007739:L Cells / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D008114:Liver Neoplasms, Experimental / Q000201:enzymology* / Q000473:pathology; D051379:Mice; D010042:Ouabain / Q000494:pharmacology*; D011188:Potassium / Q000378:metabolism; D012964:Sodium / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*
D010042:Ouabain; D012964:Sodium; D000254:Sodium-Potassium-Exchanging ATPase; D004996:Ethidium; D011188:Potassium
Vliianie uabaina na funktsiiu Na, K-ATFazy i razmnozhenie transformirovannykh kletok v kul'ture.