Intracellular survival of Candida albicans in peritoneal macrophages from chronic peritoneal dialysis patients.
Candidal peritonitis is a tenacious infection in patients undergoing chronic peritoneal dialysis. Since little is known about host defenses of the human peritoneal cavity against fungi, we investigated the interaction of peritoneal macrophages (PM phi) from uninfected dialysis patients with Candida albicans blastospores. Chemiluminescence (CL) techniques were used to assess the respiratory burst activity of these cells, and candidacidal activity was evaluated with a fluorochrome microassay. In sharp contrast to peripheral blood polymorphonuclear leukocytes (PMNs) from healthy donors, which gave a brisk luminol-enhanced CL response to opsonized blastospores and killed 35% of cell-associated organisms, PM phi produced barely detectable luminol-enhanced CL and killed only 13% of intracellular Candida. These findings appeared to be associated with a decreased level of myeloperoxidase in PM phi. The mechanism of intracellular survival of C albicans also appeared to be related to relatively poor triggering of superoxide production during phagocytosis of viable blastospores. The CL response of PMNs to C albicans was opsonin-dependent, and peritoneal dialysis effluent was devoid of opsonic activity. These studies suggest that local cellular and humoral mechanisms of defense are inadequate for protection of peritoneal dialysis patients against candidal peritonitis.
D000165:Acridine Orange; D002176:Candida albicans / Q000302:isolation & purification*; D002177:Candidiasis / Q000150:complications; D002908:Chronic Disease; D005775:Gastrula / Q000382:microbiology; D006801:Humans; D066298:In Vitro Techniques; D007424:Intracellular Fluid / Q000382:microbiology; D007962:Leukocytes / Q000382:microbiology; D008163:Luminescent Measurements; D008165:Luminol / Q000008:administration & dosage; D008264:Macrophages / Q000382:microbiology*; D009895:Opsonin Proteins / Q000032:analysis; D010529:Peritoneal Cavity / Q000382:microbiology*; D010530:Peritoneal Dialysis; D009195:Peroxidase / Q000032:analysis; D013997:Time Factors
D009895:Opsonin Proteins; D008165:Luminol; D009195:Peroxidase; D000165:Acridine Orange