The interaction of Sendai virus glycoprotein-bearing recombinant vesicles with cell surfaces.
Sendai virus glycoproteins HN and F were purified by immunoaffinity chromatography from virions disrupted by beta-D-octylglucoside. The purified glycoproteins were reconstituted in recombinant vesicles with phosphatidylcholine or phosphatidylethanolamine and phosphatidylserine. P815 or EL-4 cells treated with glycoprotein HN/F-phosphatidylcholine recombinant vesicles acquired the glycoproteins and retained them in the plasma membrane for 4 h as demonstrated by surface immunofluorescence specific for each protein. Cells treated with glycoprotein HN-phosphatidylcholine recombinant vesicles initially bore glycoprotein HN on the surface but the protein eluted within 2 h. Surfaces of cells treated with glycoprotein F-phosphatidylcholine recombinant vesicles did not acquire the glycoprotein. Cells treated with glycoprotein HN-phosphatidylethanolamine: phosphatidylserine recombinant vesicles or glycoprotein F-phosphatidylethanolamine: phosphatidylserine recombinant vesicles in the presence of 5 mM Ca2+ acquired each protein for at least 2 h. Experiments showed that the acquired glycoproteins capped with antibody and that when glycoproteins HN and F were together on the surface they co-capped. Acquired viral glycoproteins did not co-cap with intrinsic H-2 glycoproteins.
D000818:Animals; D000918:Antibody Specificity; D002118:Calcium / Q000494:pharmacology; D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D005960:Glucosides / Q000494:pharmacology; D015707:HN Protein; D006461:Hemolysis / Q000187:drug effects; D008081:Liposomes; D010222:Parainfluenza Virus 1, Human; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D013499:Surface Properties; D014759:Viral Envelope Proteins / Q000378:metabolism*; D014760:Viral Fusion Proteins; D014771:Virion / Q000032:analysis
D005960:Glucosides; D015707:HN Protein; D008081:Liposomes; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D014759:Viral Envelope Proteins; D014760:Viral Fusion Proteins; C018619:octyl-beta-D-glucoside; D002118:Calcium