Effects of spinal cord lesions and rhizotomies on cholinergic and opiate receptor binding sites in rat spinal cord.
Changes in the distribution of [3H]quinuclidinylbenzilate ([3H]QNB), [3H] alpha-bungaro-toxin ([3H]alpha-Btx) and [3H]etorphine binding sites were studied autoradiographically, and cholinacetyltransferase (ChAT) activity radioenzymatically, in the C6-7 segments of rats 1-20 days after combined dorsal and ventral C3-8 rhizotomies and spinal cord lesions at C3. After dorsal and ventral rhizotomies the number of [3H]QNB, [3H]alpha-Btx and [3H]etorphine binding sites were reduced ipsilaterally in the dorsal horn and those of [3H]QNB and [3H]alpha-Btx in the ventral horn. In the ventral horn ChAT activity was significantly reduced. After a unilateral spinal cord lesion at C3, ChAT activity was reduced in the ipsilateral ventral horn at C6-7 caudal to the lesions, whereas no change in receptor binding sites was observed.
D000818:Animals; D001345:Autoradiography; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D005048:Etorphine / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D011950:Receptors, Cholinergic / Q000378:metabolism*; D011976:Receptors, Muscarinic / Q000378:metabolism; D011978:Receptors, Nicotinic; D011957:Receptors, Opioid / Q000378:metabolism*; D013116:Spinal Cord / Q000378:metabolism / Q000502:physiology*; D013126:Spinal Nerve Roots / Q000502:physiology*; D013997:Time Factors; D064569:alpha7 Nicotinic Acetylcholine Receptor
C579165:Chrna7 protein, rat; D011950:Receptors, Cholinergic; D011976:Receptors, Muscarinic; D011978:Receptors, Nicotinic; D011957:Receptors, Opioid; D064569:alpha7 Nicotinic Acetylcholine Receptor; C028484:quinuclidinyl benzilate receptor; D005048:Etorphine; D002795:Choline O-Acetyltransferase