title: Transcription but not translation is required for EDTA-induced autolysis in Escherichia coli.
abstract: Rifampicin, but not chloramphenicol or other inhibitors of translation, inhibited EDTA-induced autolysis in Escherichia coli. Inhibition of EDTA-induced autolysis in E. coli was also observed with nalidixic acid and novobiocin, inhibitors of topoisomerase II. Rifampicin or nalidixic acid-resistant mutants of E. coli were resistant to the inhibitory effect of the respective antibiotic on EDTA-induced autolysis. The implications of these studies in regard to our understanding of the regulation of autolysis in E. coli are discussed.
mesh_terms: D001433:Bacteriolysis / Q000187:drug effects*; D002701:Chloramphenicol / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics*; D009268:Nalidixic Acid / Q000494:pharmacology; D009675:Novobiocin / Q000494:pharmacology; D014176:Protein Biosynthesis; D012293:Rifampin / Q000494:pharmacology; D013307:Streptomycin / Q000494:pharmacology; D059005:Topoisomerase II Inhibitors; D014158:Transcription, Genetic
chemical_list: D059005:Topoisomerase II Inhibitors; D009675:Novobiocin; D009268:Nalidixic Acid; D002701:Chloramphenicol; D004492:Edetic Acid; D012293:Rifampin; D013307:Streptomycin