Polyoma virus middle T gene can trigger malignant transformation of early passage rodent cells.
Previous studies on the tumourigenic conversion of early passage rat embryo cells by the polyoma virus early genes have suggested a multigenic control of tumourigenesis. Thus, the large T gene can immortalize early passage rat cells and can relieve the serum dependence of normal and transformed cells. The middle T gene alone cannot immortalize early passage cells; however, it can induce cells of established cell lines to become anchorage-independent and tumourigenic. Here we show that when linked to transcriptional enhancers, the polyoma virus middle T gene can trigger the complete malignant transformation of early passage rodent cells. Therefore, the polyoma virus middle T gene does not require a cooperating oncogene to induce malignant conversion of these cells.
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000952:Antigens, Polyomavirus Transforming; D000957:Antigens, Viral, Tumor / Q000235:genetics*; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002472:Cell Transformation, Viral; D006224:Cricetinae; D004274:DNA, Recombinant; D004351:Drug Resistance; D005814:Genes, Viral; D005839:Gentamicins / Q000494:pharmacology; D008168:Lung; D009856:Oncogene Proteins, Viral / Q000235:genetics*; D009857:Oncogenes; D011120:Polyomavirus / Q000235:genetics*; D014162:Transfection
D000900:Anti-Bacterial Agents; D000952:Antigens, Polyomavirus Transforming; D000957:Antigens, Viral, Tumor; D004274:DNA, Recombinant; D005839:Gentamicins; D009856:Oncogene Proteins, Viral; C010680:antibiotic G 418