[Preparation of iodinated standards for quantitative radioautography in vitro using a tritium-sensitive film].
In the present study we developed iodinated 125I-standards and tested the response of tritium-sensitive sheet film (3H-Ultrofilm) to varying concentrations of 125I prepared from brain "paste" mixed with mono-iodinated TiTx gamma toxin. Results have practical implications since they allow quantitative measurements of autoradiograms obtained with 125I-coordinats. An example is shown for 125I-neurotensin binding sites on rat brain sections.
D000818:Animals; D001345:Autoradiography / Q000379:methods; D001921:Brain / Q000378:metabolism*; D007457:Iodine Radioisotopes; D007700:Kinetics; D009496:Neurotensin / Q000378:metabolism*; D051381:Rats; D018028:Receptors, Neurotensin; D017981:Receptors, Neurotransmitter / Q000378:metabolism*; D014018:Tissue Distribution; D014316:Tritium
D007457:Iodine Radioisotopes; D018028:Receptors, Neurotensin; D017981:Receptors, Neurotransmitter; D014316:Tritium; D009496:Neurotensin
Préparation de standards iodés pour radioautographie quantitative in vitro à l'aide d'un film sensible au tritium.