Elimination of the "chromogen oxidase" activity of bilirubin oxidase added to obviate bilirubin interference in hydrogen peroxide/peroxidase detecting systems.
The use of bilirubin oxidase to remove interference by bilirubin in hydrogen peroxide/peroxidase detecting systems is hampered by its inherent "chromogen oxidase" activity (its ability to oxidize the chromogens used in the systems). This unwanted activity is greater than 99% inhibited by 0.5 mmol/L cyanide, 97% inhibited by 20 mmol/L azide. At these same concentrations, they inhibit bilirubin oxidase activity by 95% and 73%, respectively. Sequential addition of reagents allows the use of bilirubin oxidase without interference by the chromogen oxidase activity.
D015720:5'-Nucleotidase; D001663:Bilirubin; D002384:Catalysis; D002863:Chromogenic Compounds / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000032:analysis*; D007202:Indicators and Reagents; D009708:Nucleotidases; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000378:metabolism*; D044925:Oxidoreductases Acting on CH-CH Group Donors; D010544:Peroxidases / Q000032:analysis*
D002863:Chromogenic Compounds; D007202:Indicators and Reagents; D006861:Hydrogen Peroxide; D010088:Oxidoreductases; D010544:Peroxidases; D044925:Oxidoreductases Acting on CH-CH Group Donors; C042401:bilirubin oxidase; D009708:Nucleotidases; D015720:5'-Nucleotidase; D001663:Bilirubin