Effects of 8-bromoadenosine 3':5'-cyclic monophosphate on proteolytic enzymes, adhesiveness and lung-colonizing ability of cloned low-metastatic Lewis lung carcinoma cells.
Treatment of cloned low-metastatic Lewis lung carcinoma cells (P-29) with dimethylsulfoxide or butyric acid resulted in enhancement of their lung-colonizing ability. This was accompanied with increases in cathepsin B activity, the production of plasminogen activator, and adhesiveness, mainly heterotypic adhesion (adhesion to monolayers of endothelial cells) of dimethylsulfoxide-treated cells and homotypic aggregation of butyric acid-treated cells. Treatment of P-29 cells with 8-bromoadenosine 3':5'-cyclic monophosphate (8-bromo-cyclic AMP) also resulted in increases in cathepsin B activity and the production of plasminogen activator. However, it did not enhance either heterotypic adhesion or homotypic aggregation of the cells. The lung-colonizing ability of 8-bromo-cyclic AMP-treated P-29 cells was examined after their intravenous injection into male C57BL/6 mice. It was found that these cells did not have enhanced lung-colonizing ability. These results suggest that high activities of proteolytic enzymes such as cathepsin B and plasminogen activator in tumor cells are not sufficient alone for completing the metastatic process, but that other properties of tumor cells such as adhesiveness are also necessary.
D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology*; D002401:Cathepsin B / Q000032:analysis*; D002448:Cell Adhesion; D002460:Cell Line; D002999:Clone Cells; D000242:Cyclic AMP / Q000502:physiology; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D008175:Lung Neoplasms / Q000201:enzymology / Q000473:pathology*; D010960:Plasminogen Activators / Q000096:biosynthesis*
D015124:8-Bromo Cyclic Adenosine Monophosphate; D000242:Cyclic AMP; D010960:Plasminogen Activators; D002401:Cathepsin B; D004121:Dimethyl Sulfoxide