A variable tandem repeat locus mapped to chromosome band 10q26 is amplified and rearranged in leukocyte DNAs of two cancer patients.
A highly polymorphic locus associated with the variable tandem repetition of a 35 bp consensus sequence was mapped to chromosome 10, band q26. Examination of leukocyte DNA from a cancer patient revealed the twenty-fold amplification of one allelic fragment of this locus, while the other allelic fragment demonstrated a normal copy number. In another patient, Southern blotting of leukocyte DNA detected the deletion of the 3'-flanking region from one tandem repeat allele. These results indicate that variable tandem repeats may mark highly unstable regions of DNA in the human genome which can be altered by changes more extensive than simple tandem repeat variation.
D001483:Base Sequence; D002879:Chromosomes, Human, Pair 10; D003110:Colonic Neoplasms / Q000235:genetics; D004262:DNA Restriction Enzymes; D004273:DNA, Neoplasm / Q000235:genetics*; D004276:DNA, Satellite / Q000235:genetics*; D005784:Gene Amplification; D006801:Humans; D007962:Leukocytes / Q000502:physiology; D012004:Rectal Neoplasms / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid; D001749:Urinary Bladder Neoplasms / Q000235:genetics
D004273:DNA, Neoplasm; D004276:DNA, Satellite; D004262:DNA Restriction Enzymes