Cleavage site specificity of vertebrate collagenases.
A series of synthetic peptide substrates for vertebrate collagenase having the structure Ac-Pro-Leu-Gly-X-Leu-Gly-OC2H5, where X is Leu, Ile, Val, Phe and Ala, have been prepared. Collagenolytic enzymes from various sources cleave these substrates with differing relative rate patterns. This series of peptides should be valuable for characterization of collagenases.
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D005260:Female; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism*; D007700:Kinetics; D051379:Mice; D003012:Microbial Collagenase / Q000378:metabolism*; D011817:Rabbits; D051381:Rats; D013329:Structure-Activity Relationship; D013379:Substrate Specificity
D007527:Isoenzymes; D003012:Microbial Collagenase