Isolation of human pathogenic viruses from clinical material on CaCo2 cells.
The use of the epidermal-like cell line CaCo2 and the efficient propagation of viruses from clinical material for diagnostic purposes are reported. Virus replication was observed by cytopathic effects and/or immunofluorescence. The following viruses replicate in CaCo2 cells: enteroviruses (coxsackie B1-B6, poliovirus types 1-3, most echoviruses and coxsackie A viruses), adenoviruses, herpes simplex types 1 and 2, measles, respiratory syncytial, parainfluenza type 2 viruses, and to a lesser extent rubella and mumps viruses. CaCo2 cells were used in neutralization tests for the diagnosis of enteroviral infections.
D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D004770:Enterovirus / Q000254:growth & development / Q000302:isolation & purification*; D005455:Fluorescent Antibody Technique; D006801:Humans; D009500:Neutralization Tests; D014776:Virus Cultivation; D014777:Virus Diseases / Q000175:diagnosis / Q000382:microbiology; D014779:Virus Replication; D014780:Viruses / Q000254:growth & development / Q000302:isolation & purification*