A comparison of the acid-soluble polypeptides of five herpesviruses.
The polypeptides soluble in 0.25 M-HCl were extracted from the nuclei of BHK cells infected with herpes simplex virus type 1 or type 2 and separated by SDS-PAGE. Seventeen polypeptides were detectable in each extract of which 10 type 1 and nine type 2 polypeptides were reproducibly effectively extracted. In cells infected with bovine mammillitis virus, pseudorabies virus or equine herpesvirus type 1, at least 12, 13 and eight polypeptides respectively were acid-soluble. In addition to histones, three other cellular polypeptides were present in sizeable quantities in the acid extracts and could obscure other acid-soluble viral polypeptides. Possible relationships between some polypeptides of the five herpesviruses are discussed.
D000818:Animals; D002460:Cell Line; D002467:Cell Nucleus / Q000032:analysis; D006224:Cricetinae; D006564:Herpesviridae / Q000032:analysis* / Q000254:growth & development; D004861:Herpesvirus 1, Equid / Q000032:analysis / Q000254:growth & development; D011558:Herpesvirus 1, Suid / Q000032:analysis / Q000254:growth & development; D001911:Herpesvirus 2, Bovine / Q000032:analysis / Q000254:growth & development; D006657:Histones / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D007668:Kidney; D008970:Molecular Weight; D010455:Peptides / Q000032:analysis / Q000302:isolation & purification; D018139:Simplexvirus / Q000032:analysis / Q000254:growth & development; D012995:Solubility; D014764:Viral Proteins / Q000032:analysis* / Q000302:isolation & purification
D006657:Histones; D010455:Peptides; D014764:Viral Proteins