Neurovirulence and latency in inbred mice of two HSV-1 intrastrain variants of divergent pathogenicity.
The pathogenicity pattern of the HSV-1 strain ANG which is nonencephalitogenic in mice is compared with that of a selected neurovirulent variant of this strain in DBA-2 mice. After i.p. inoculation both variants replicate to high titers in the mouse peritoneum and build up a virus reservoir in the spleen. Both viruses have no effect on visceral mouse organs other than the spleen; both viruses lead to an inefficient and masked viraemia and both replicate efficiently in CNS tissue after direct intracranial injection. Only the pathogenic variant, however, spreads to the CNS and leads to lethal encephalitis upon intraperitoneal infection. The assumption that infection of the CNS would be mediated by hematogenous transport is not supported by the data obtained from transfer and cocultivation experiments with lymphocytes or experiments involving artificial viraemia. In a model to analyse the capacity of the viruses to invade nerve axons and to induce a latent infection both viruses were found to be latency positive in dorsal root ganglia. It is clear that non-neurovirulent HSV-1 strains are subjected to a postganglionic block of virus spread from the periphery to the CNS. The experiments led to the hypothesis that axonal transport even beyond the dorsal root ganglia to the CNS proceeds unrestricted, whereas lethal CNS invasion is prevented by a restriction of viral replication of HSV-1 ANG in the CNS by a virus-induced host defence mechanism.
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002460:Cell Line; D002522:Chlorocebus aethiops; D006561:Herpes Simplex / Q000382:microbiology*; D007668:Kidney; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D009422:Nervous System Diseases / Q000382:microbiology*; D018139:Simplexvirus / Q000235:genetics / Q000472:pathogenicity*; D013045:Species Specificity; D014774:Virulence
D000956:Antigens, Viral