A DNA glycosylase for oxidized thymine residues in Drosophila melanogaster.
A DNA glycosylase activity which excises fragmented thymine residues has been identified in cell extracts from Drosophila melanogaster embryos. The enzyme has an apparent Mr = 40,000, acts preferentially on double-stranded polydeoxyribonucleotide substrate and requires no co-factors. The DNA glycosylase presumably acts in excision-repair of pyrimidines damaged by hydroxyl radicals and other oxidizing species. This is the first identification of a DNA glycosylase in Drosophila cells.
D000818:Animals; D045647:DNA Glycosylases; D004260:DNA Repair; D004331:Drosophila melanogaster; D006878:Hydroxides; D017665:Hydroxyl Radical; D008970:Molecular Weight; D009699:N-Glycosyl Hydrolases / Q000378:metabolism*; D013329:Structure-Activity Relationship; D013941:Thymine / Q000378:metabolism*
D006878:Hydroxides; D017665:Hydroxyl Radical; D045647:DNA Glycosylases; D009699:N-Glycosyl Hydrolases; D013941:Thymine