title: Effect of glutaraldehyde on the activity of some DNA restriction endonucleases.
abstract: The effect of the bifunctional crosslinking reagent glutaraldehyde on the activity of the restriction enzymes Bam HI, Hind III, EcoRI, and Tthlll I was investigated. The four enzymes exhibited differential sensitivity to inactivation. Tthlll I was the most sensitive, with activity losses occurring at levels of 0.0025% and above. Hind III was the most stable of the four and remained fully active at concentrations as high as 0.075%. Addition of BSA to incubation mixtures generally had a stabilizing effect. Implications of these results for the design of glutaraldehyde-based methods for the immobilization of restriction endonucleases are discussed.
mesh_terms: D000447:Aldehydes / Q000494:pharmacology*; D002021:Buffers; D003432:Cross-Linking Reagents; D004262:DNA Restriction Enzymes / Q000037:antagonists & inhibitors*; D004355:Drug Stability; D004591:Electrophoresis, Polyacrylamide Gel; D005976:Glutaral / Q000494:pharmacology*; D066298:In Vitro Techniques; D012710:Serum Albumin, Bovine; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000447:Aldehydes; D002021:Buffers; D003432:Cross-Linking Reagents; D012710:Serum Albumin, Bovine; D004262:DNA Restriction Enzymes; D005976:Glutaral