Demonstration of virus-specific transcriptional activator(s) in cells infected with HTLV-III by an in vitro cell-free system.
Human T cell lymphotropic viruses (HTLV's) differ from most other retroviruses based on the presence of trans-regulatory genes for virus expression. In this study, using an in vitro cell-free transcription system, we demonstrate that nuclear extracts obtained from cells infected with HTLV-III (human immunodeficiency virus, HIV) contain a factor(s) that stimulates transcription specifically from the HTLV-III promoter. Present studies indicate that this activation is mainly involved in the initiation of transcription from the HTLV-III promoter.
D002460:Cell Line; D002467:Cell Nucleus / Q000032:analysis; D002474:Cell-Free System; D017977:Deltaretrovirus / Q000235:genetics*; D005786:Gene Expression Regulation; D005814:Genes, Viral; D006801:Humans; D009690:Nucleic Acid Conformation; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D012191:Retroviridae Proteins / Q000235:genetics / Q000502:physiology; D014158:Transcription, Genetic
D012191:Retroviridae Proteins