Invasion potential of human choriocarcinoma cell lines and the role of lytic enzymes.
The chick chorioallantoic membrane (CAM) was used as an assay system to examine the invasive potential of human choriocarcinoma cell lines. When 5 X 10(6) cells were inoculated into the CAM at the 10th day of postfertilization, three of eight cell lines formed extensively invasive tumors within the CAM. A tendency to correlation between the tumorigenic potential of cell lines in hamster cheek pouches and their invasive potential in the CAM was noted. In addition, the invasive capacity of cell lines correlated well with the amount of collagenase but did not correlate with the amount of plasminogen activator or cathepsin B secreted by them. It is concluded that a heterogeneity of invasive potential in the CAM exists among human choriocarcinoma cell lines and the role of the collagenase secreted by them is suggested.
D000482:Allantois / Q000473:pathology; D000818:Animals; D002401:Cathepsin B; D002403:Cathepsins / Q000378:metabolism*; D002460:Cell Line; D002642:Chick Embryo; D002822:Choriocarcinoma / Q000201:enzymology / Q000378:metabolism / Q000473:pathology*; D002823:Chorion / Q000473:pathology; D006063:Chorionic Gonadotropin / Q000378:metabolism; D006224:Cricetinae; D005260:Female; D006801:Humans; D008647:Mesocricetus; D003012:Microbial Collagenase / Q000378:metabolism*; D009061:Mouth Mucosa / Q000473:pathology; D009361:Neoplasm Invasiveness; D010960:Plasminogen Activators / Q000378:metabolism*; D011247:Pregnancy; D014594:Uterine Neoplasms / Q000201:enzymology / Q000378:metabolism / Q000473:pathology*
D006063:Chorionic Gonadotropin; D002403:Cathepsins; D010960:Plasminogen Activators; D002401:Cathepsin B; D003012:Microbial Collagenase