Serum free cultivation of lymphoid cells for virus and interferon production.
Minor modifications of standard media have given a defined serum free medium for the production of polio virus and measles virus in lymphoblastoid cells and of Namalva interferon in lymphoma cells. By usage of slightly modified standard roller equipment in the order of 2 X 10 12TCID-50 of polio virus and 4 X 10(9) IU of interferon can be produced on one roller rack. The produced poliovirus does comply with requirements for inactivated poliovaccine.
D002460:Cell Line; D003470:Culture Media; D003584:Cytological Techniques; D006801:Humans; D007370:Interferon Type I / Q000096:biosynthesis*; D008214:Lymphocytes / Q000276:immunology / Q000382:microbiology; D008459:Measles virus / Q000302:isolation & purification; D017955:Poliovirus / Q000302:isolation & purification; D014776:Virus Cultivation / Q000379:methods*
D003470:Culture Media; D007370:Interferon Type I