Reconstitution in bilayer lipid membranes of the crab Potamon transcaspicum spider venom sensitive glutamate receptors.
Membrane proteins have been isolated from neuromuscular synapses of the crab Potamon transcaspicum using a specific blocker of glutamatergic synapses, the neurotoxin of the spider Argiope lobata. These membrane components have been shown to induce glutamate-sensitive conductance in bilayer lipid membranes (BLM) in the presence of sodium ions. As an agent blocking desensitization of glutamatergic synapses, concanavalin A was shown to enhance the conductance and to abolish desensitization. Diethylester of glutamic acid as a blocker of glutamatergic synapses inhibited glutamate-induced conductance. No similar change in conductance was seen when BLM was modified by a membrane proteins -neurotoxin complex. Conductance current fluctuations induced by these receptor protein components were monitored by the single-channel registration method.
D000818:Animals; D001180:Arthropod Venoms / Q000494:pharmacology*; D003386:Brachyura; D003208:Concanavalin A / Q000494:pharmacology; D004553:Electric Conductivity; D005971:Glutamates / Q000378:metabolism*; D007700:Kinetics; D008051:Lipid Bilayers; D017470:Receptors, Glutamate; D017981:Receptors, Neurotransmitter / Q000187:drug effects / Q000502:physiology*; D013111:Spider Venoms / Q000494:pharmacology*
D001180:Arthropod Venoms; D005971:Glutamates; D008051:Lipid Bilayers; D017470:Receptors, Glutamate; D017981:Receptors, Neurotransmitter; D013111:Spider Venoms; D003208:Concanavalin A