title: A reversible effect of low carbon monoxide concentrations on the EPR spectra of the periplasmic hydrogenase from Desulfovibrio vulgaris.
abstract: The effect of low concentrations of CO (0.93 - 5.58 microM) on the EPR spectrum of the periplasmic non-heme iron hydrogenase from D. vulgaris has been investigated. The "g = 2.06" EPR signal is maximally induced (0.94 spin/molecule) at 46.5 microM CO and partial induction of the EPR signal could be observed at 0.93 microM CO. This effect is reversed by removal of the CO or irradiation of the hydrogenase with white light.
mesh_terms: D002248:Carbon Monoxide; D003901:Desulfovibrio / Q000201:enzymology*; D004578:Electron Spin Resonance Spectroscopy; D006859:Hydrogen; D006864:Hydrogenase / Q000037:antagonists & inhibitors / Q000528:radiation effects; D008027:Light; D010084:Oxidation-Reduction
chemical_list: D002248:Carbon Monoxide; D006859:Hydrogen; D006864:Hydrogenase