title: Isolation and characterization of sarcoplasmic reticulum from normal and dystrophic chicken.
abstract: Purified sarcoplasmic reticulum vesicles from normal and dystrophic chicken skeletal muscle have been isolated by a procedure employing pressure disruption of a microsomal suspension. The dystrophic sarcoplasmic reticulum (SR) exhibited reduced Ca++ transport and phosphoenzyme formation, but the Ca++-ATPase activity was normal. Normal and dystrophic SR showed similar lipid profiles, except for a significant increase in free fatty acids in the dystrophic SR. Investigations involving the interaction of oleic acid with normal SR showed fatty acids can induce conditions similar to those found in dystrophic SR.
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000378:metabolism; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D002458:Cell Fractionation; D002499:Centrifugation, Density Gradient; D002645:Chickens; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D007700:Kinetics; D008563:Membrane Lipids / Q000032:analysis; D009132:Muscles / Q000201:enzymology / Q000648:ultrastructure*; D009137:Muscular Dystrophy, Animal / Q000201:enzymology / Q000473:pathology*; D010042:Ouabain / Q000378:metabolism; D012519:Sarcoplasmic Reticulum / Q000201:enzymology / Q000648:ultrastructure*; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism
chemical_list: D008563:Membrane Lipids; D010042:Ouabain; D002795:Choline O-Acetyltransferase; D000251:Adenosine Triphosphatases; D000252:Calcium-Transporting ATPases; D000254:Sodium-Potassium-Exchanging ATPase; D002118:Calcium