Flying squirrel-associated Rickettsia prowazekii (epidemic typhus rickettsiae) characterized by a specific DNA fragment produced by restriction endonuclease digestion.
The DNA from flying squirrel-associated Rickettsia prowazekii was characterized by using a specific DNA fragment produced by digestion with the enzyme BamHI. The DNA fragment was cloned into a plasmid vector and used to readily distinguish between available human- and flying squirrel-associated R. prowazekii DNAs derived from crude cytoplasmic extracts.
D000818:Animals; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000032:analysis*; D015245:Deoxyribonuclease BamHI; D006801:Humans; D009693:Nucleic Acid Hybridization; D012283:Rickettsia prowazekii / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012589:Sciuridae / Q000382:microbiology*
D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes; D015245:Deoxyribonuclease BamHI