title: Effect of mixtures of dietary fibres on the enzyme activity of the rat caecal microflora.
abstract: The enzyme activity of the caecal microflora from weanling rats was determined after feeding 1 of 3 basal diets (purified fibre-free; purified plus cellulose; and stock), with or without additional dietary fibre (pectin, i-carrageenan or carboxymethylcellulose 5% w/w). The wet weight of caecal contents and total bacterial numbers were similar for the purified fibre-free and purified plus cellulose diets, yet were significantly higher in animals fed the stock diet. Pectin supplementation of the basal diets had no effect of caecal bacterial numbers, but significantly increased total nitrate reductase activity per caecum except when added to stock diet. Carrageenan decreased caecal bacterial numbers and most enzyme activities with both purified diets, and to a lesser extent with the stock diet. Carboxymethylcellulose increased bacterial numbers and enzyme activities, particularly beta-glucosidase and nitrate reductase when added to the purified diet but not when added to either the purified diet plus cellulose or the stock diet. The results demonstrate that the effects of dietary fibre components on the rat caecal microflora are dependent upon the initial fibre content of the diet base.
mesh_terms: D000818:Animals; D001419:Bacteria / Q000201:enzymology*; D001835:Body Weight / Q000187:drug effects; D002266:Carboxymethylcellulose Sodium / Q000494:pharmacology; D002351:Carrageenan / Q000494:pharmacology; D002432:Cecum / Q000187:drug effects / Q000201:enzymology / Q000382:microbiology*; D004043:Dietary Fiber / Q000494:pharmacology*; D005766:Gastrointestinal Contents; D008297:Male; D010368:Pectins / Q000494:pharmacology; D051381:Rats
chemical_list: D004043:Dietary Fiber; D010368:Pectins; D002351:Carrageenan; D002266:Carboxymethylcellulose Sodium