Production of high titre human interferon-gamma in primed leukocyte cultures.
Con A-stimulated leukocytes previously treated with IFN-alpha (1500 IU/ml for 4 h) were very suitable for the production of IFN-gamma with a high titre even under large-scale conditions. The properties of the IFN produced under such conditions were identical to those of IFN-gamma. The IFN-gamma produced in primed cultures showed two peaks of antiviral activity corresponding to molecular weights of 51 000 and 23 000 daltons.
D002294:Carcinoma, Squamous Cell; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D006801:Humans; D007370:Interferon Type I / Q000633:toxicity; D007371:Interferon-gamma / Q000096:biosynthesis* / Q000633:toxicity; D007962:Leukocytes / Q000187:drug effects / Q000276:immunology*; D010222:Parainfluenza Virus 1, Human / Q000276:immunology
D007370:Interferon Type I; D003208:Concanavalin A; D007371:Interferon-gamma