title: Chemiluminescence of luminol caused by interaction of cytochrome P-450 and cytochrome C with cumene hydroperoxide: comparative studies.
abstract: A comparative study of the interaction of cumene hydroperoxide with cytochrome P-450LM2 and with cytochrome C has been undertaken using the chemiluminescence method in the presence of luminol. Considerable differences in the mechanisms of action of both hemoproteins have been revealed with various inhibitors of radical stages, i.e. superoxide dismutase, mannitol, sodium azide, and alpha-tocopherol. It is shown that molecular oxygen participates in the process of hemoprotein-catalyzed hydroperoxide oxidation of luminol.
mesh_terms: D000818:Animals; D001555:Benzene Derivatives / Q000378:metabolism*; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D003574:Cytochrome c Group / Q000378:metabolism*; D007700:Kinetics; D008099:Liver / Q000378:metabolism; D008163:Luminescent Measurements; D008165:Luminol; D011817:Rabbits; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D001555:Benzene Derivatives; D003574:Cytochrome c Group; D008165:Luminol; D003577:Cytochrome P-450 Enzyme System; D013482:Superoxide Dismutase; C007164:cumene hydroperoxide