title: Nucleotide sequence of cDNA clones encoding the outer capsid protein, VP5, of bluetongue virus serotype 10.
abstract: The complete sequence of the RNA segment that codes for a major outer capsid protein (VP5) of bluetongue virus serotype 10 has been determined from overlapping cDNA clones inserted into pBR322. The segment 5 RNA of the virus (M5 RNA) is deduced to be 1638 base pairs long (1.05 X 10(6) daltons) and has an open reading frame in one strand capable of coding for a protein with a calculated size of 59 163 daltons (526 amino acids) and a net charge of -4.5 at neutral pH.
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001483:Base Sequence; D001820:Bluetongue virus / Q000145:classification / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis*; D004262:DNA Restriction Enzymes; D005796:Genes; D005814:Genes, Viral; D012087:Reoviridae / Q000235:genetics*; D012703:Serotyping; D014764:Viral Proteins / Q000235:genetics*; D015678:Viral Structural Proteins
chemical_list: D000596:Amino Acids; D014764:Viral Proteins; D015678:Viral Structural Proteins; D004247:DNA; D004262:DNA Restriction Enzymes