title: Effects of sodium n-butyrate on entry into S phase in resting rat 3Y1 cells infected with simian virus 40.
abstract: In quiescent rat 3Y1 fibroblasts infected with simian virus 40 (SV40), sodium butyrate elongated the time lag before entry into S phase in a concentration-dependent fashion. In spite of the elongated time lags, SV40-infected cells entered S phase in a very synchronous mode, irrespective of the butyrate concentrations. The elongated time lag seemed to be at least partially due to a delayed synthesis and a delayed accumulation of large T antigen caused by butyrate. The entry into S phase was also delayed even when butyrate was added to the cultures after expression of T antigen to an extent sufficient for untreated cells to enter S phase. This suggests that butyrate may also inhibit a cellular event(s) that is required for entry into S phase after expression of the T antigen. In contrast, serum-stimulated cells were more sensitive to butyrate with respect to entry into S phase than SV40-infected cells, and the distribution of the time lag among cell populations increased (i.e., asynchrony in entry into S phase increased) with an increase in the butyrate concentration.
mesh_terms: D000818:Animals; D000957:Antigens, Viral, Tumor / Q000235:genetics; D002087:Butyrates / Q000494:pharmacology*; D020148:Butyric Acid; D002453:Cell Cycle / Q000187:drug effects*; D002460:Cell Line; D002472:Cell Transformation, Viral / Q000187:drug effects; D004261:DNA Replication / Q000187:drug effects; D003609:Dactinomycin / Q000494:pharmacology; D006133:Growth Substances; D007399:Interphase / Q000187:drug effects; D051381:Rats; D013539:Simian virus 40
chemical_list: D000957:Antigens, Viral, Tumor; D002087:Butyrates; D006133:Growth Substances; D020148:Butyric Acid; D003609:Dactinomycin