Use of immunosorbent electron microscopy for detection of rota- and hepatitis A virus in sucrose solutions.
Immunosorbent electron microscopy was used to demonstrate rotavirus in solutions of varying sucrose concentrations after 18, 42 and 66 h of incubation. About 50% of adsorption of virus particles to the grid was achieved after 18 h incubation and nearly 100% after 42 h when compared to trapping of virus from sucrose free solutions. Hepatitis A virus was purified in a 10-30% sucrose gradient and each fraction was examined by immunosorbent electron microscopy, direct electron microscopy, immune electron microscopy and radioimmunoassay. The sensitivities of immunosorbent electron microscopy and radioimmunoassay were essentially similar and considerably greater than direct electron microscopy and conventional immune electron microscopy.
D000914:Antibodies, Viral; D000956:Antigens, Viral / Q000032:analysis; D006507:Hepatovirus / Q000276:immunology / Q000302:isolation & purification*; D007106:Immune Sera; D007163:Immunosorbent Techniques; D008854:Microscopy, Electron; D011863:Radioimmunoassay; D012401:Rotavirus / Q000276:immunology / Q000302:isolation & purification*; D013395:Sucrose
D000914:Antibodies, Viral; D000956:Antigens, Viral; D007106:Immune Sera; D013395:Sucrose