Inhibition of fructose 1,6-bisphosphatase from pig liver by fructose 2,6-bisphosphate.
The inhibition of pig liver fructose 1,6-bisphosphatase by fructose 2,6-bisphosphate has been investigated over a wide range of substrate concentration by measuring the release of labelled inorganic phosphate from [1-32P]fructose 1,6-bisphosphate. The activity of the enzyme can be inhibited completely by fructose 2,6-bisphosphate. The inhibiting effect is most pronounced at low substrate concentrations. The results have been analyzed in terms of a mathematical model assuming a competitive interaction of fructose 1,6-bisphosphate and fructose 2,6-bisphosphate at the catalytic site as well as a synergistic cooperation of the two hexose bisphosphates at an inhibiting allosteric site of the enzyme.
D000818:Animals; D006597:Fructose-Bisphosphatase / Q000037:antagonists & inhibitors*; D005635:Fructosediphosphates / Q000494:pharmacology*; D006598:Hexosediphosphates / Q000494:pharmacology*; D066298:In Vitro Techniques; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008954:Models, Biological; D013552:Swine
D005635:Fructosediphosphates; D006598:Hexosediphosphates; D006597:Fructose-Bisphosphatase; C029063:fructose-1,6-diphosphate