Flow cytometric analysis of hepatoma tissue and HeLa cells grown on various types of microbeads using hydroxyurea, nocodazole and aphidicolin in succession.
Applying Hydroxyurea, Nocodazole and Aphidicolin in succession to obtain parasynchronous growth, the progression of HTC and HeLa cells through the cell cycle has been monitored by laser flow cytometry. The experimental results show that HTC cells behave identically whether grown in monolayer or attached to dextran-based microbeads but that the chemical nature of the micro-support itself plays an important role especially on the speed with which the cells pass from mitosis into G1, polyacrylamide-based microbeads being superior in this respect.
D016590:Aphidicolin; D001562:Benzimidazoles; D006528:Carcinoma, Hepatocellular / Q000473:pathology*; D002453:Cell Cycle; D002455:Cell Division; D003584:Cytological Techniques; D004224:Diterpenes; D005434:Flow Cytometry; D006367:HeLa Cells / Q000166:cytology*; D006801:Humans; D006918:Hydroxyurea; D007700:Kinetics; D008113:Liver Neoplasms / Q000473:pathology*; D008863:Microspheres; D015739:Nocodazole
D001562:Benzimidazoles; D004224:Diterpenes; D016590:Aphidicolin; D015739:Nocodazole; D006918:Hydroxyurea