Antimicrobial properties of hydrogen peroxide and sodium bicarbonate individually and in combination against selected oral, gram-negative, facultative bacteria.
The topical application of hydrogen peroxide (H2O2) and sodium bicarbonate (NaHCO3), individually and in combination, has been used empirically in the treatment of periodontal diseases. In this study, we examined both minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC) of these disinfectants individually and in combination against selected facultative, Gram-negative oral bacteria in a microtiter dilution assay. The bacteria studied included Actinobacillus actinomycetemcomitans, Haemophilus aphrophilus, Eikenella corrodens, and Capnocytophaga gingivalis. These bacteria exhibited MBC (one hr) values ranging from 75 mumol/L to greater than 10 mmol/L and MIC from less than 5 to 500 mumol/L for H2O2. The tested bacteria exhibited MIC values for NaHCO3 of from 23 to 182 mmol/L, and the MBC (one hr) exceeded 728 mmol/L for most of the strains examined. At sublethal (sub-MIC) concentrations, sodium bicarbonate antagonized the ability of H2O2 to inhibit bacterial growth in MIC assays, but sublethal concentrations of H2O2 had no effect on the MIC values of NaHCO3. Lethal concentrations of H2O2 and NaHCO3 exhibited synergistic antimicrobial activity in combination in one-hour bactericidal assays. Since the bactericidal properties of these antimicrobial agents are synergistic, we conclude that it may be rational to use them in combination to treat certain forms of periodontal disease. Also, lower and perhaps safer concentrations of H2O2 can be used in combination with NaHCO3 when oxidative antimicrobial chemotherapy is indicated.
D000188:Actinobacillus / Q000187:drug effects; D000891:Anti-Infective Agents, Local; D001639:Bicarbonates / Q000008:administration & dosage / Q000494:pharmacology*; D002206:Capnocytophaga / Q000187:drug effects; D004336:Drug Antagonism; D004338:Drug Combinations; D004357:Drug Synergism; D016124:Eikenella corrodens / Q000187:drug effects; D006089:Gram-Negative Anaerobic Bacteria / Q000187:drug effects*; D006190:Haemophilus / Q000187:drug effects; D006801:Humans; D006861:Hydrogen Peroxide / Q000008:administration & dosage / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D010519:Periodontium / Q000382:microbiology; D012964:Sodium / Q000008:administration & dosage / Q000494:pharmacology*; D017693:Sodium Bicarbonate
D000891:Anti-Infective Agents, Local; D001639:Bicarbonates; D004338:Drug Combinations; D017693:Sodium Bicarbonate; D012964:Sodium; D006861:Hydrogen Peroxide