Quantitation of zinc in nitric acid-digested plasma by atomic absorption spectrophotometry.
Human plasma, digested in a screw-capped Teflon vial for 1 h at 130 degrees C in concentrated nitric acid, is assayed for zinc by atomic absorption spectrophotometry using a single-slot, 10-cm burner and air/acetylene flame. The assay is linear to 10.00 mg Zn/liter, recovery averages 100.9%, and inter- and intraassay coefficients of variation are 5.9 and 1.9%. With this method, there is no burner clogging or adjustment necessary for sample viscosity. Sodium chloride does not interfere with the assay. The linear regression data of the standard curve for milligrams of Zn per liter (x) and milliabsorbance units (y) is y = 40x + 0.001.
D006801:Humans; D008832:Microchemistry; D009566:Nitrates; D017942:Nitric Acid; D013054:Spectrophotometry, Atomic / Q000379:methods; D015032:Zinc / Q000097:blood*
D009566:Nitrates; D017942:Nitric Acid; D015032:Zinc