Enzymatic synthesis of [beta-32P]ADP using adenylate kinase and [gamma-32P]ATP.
An enzymatic method for the synthesis of [beta-32P]ADP from [gamma-32P]ATP is described. This substrate is required for the assay of ADPase and is not commercially available. The method described results in a preparation of [beta-32P]ADP of high purity with a yield of approximately 40% the theoretical obtainable.
D000244:Adenosine Diphosphate / Q000138:chemical synthesis*; D000255:Adenosine Triphosphate; D000263:Adenylate Kinase; D006863:Hydrogen-Ion Concentration; D007553:Isotope Labeling / Q000379:methods*; D007700:Kinetics; D010761:Phosphorus Radioisotopes; D010770:Phosphotransferases
D010761:Phosphorus Radioisotopes; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D010770:Phosphotransferases; D000263:Adenylate Kinase