Effects of chloramphenicol on the long term trophic action of ACTH on rat adrenocortical cells: a combined stereological and enzymological study.
Chronic chloramphenicol administration was found to block the ACTH-induced increase in both the surface area of mitochondrial cristae and the activity of 11 beta-hydroxylase in zona fasciculata cells of the rat adrenal cortex. The surface area of agranular endoplasmic reticulum membranes and the activity of 3 beta-hydroxysteroid dehydrogenase were not affected by treatment with chloramphenicol. These findings suggest that the mechanism of the ACTH-induced enhancement of the growth and steroidogenic capacity of rat adrenocortical cells involves stimulation of mitochondrial DNA-dependent protein synthesis.
D015096:3-Hydroxysteroid Dehydrogenases / Q000378:metabolism; D000302:Adrenal Cortex / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology; D000324:Adrenocorticotropic Hormone / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D000818:Animals; D002701:Chloramphenicol / Q000494:pharmacology*; D004721:Endoplasmic Reticulum / Q000187:drug effects; D006984:Hypertrophy; D008297:Male; D008928:Mitochondria / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains; D013252:Steroid 11-beta-Hydroxylase / Q000378:metabolism; D013997:Time Factors
D002701:Chloramphenicol; D000324:Adrenocorticotropic Hormone; D015096:3-Hydroxysteroid Dehydrogenases; D013252:Steroid 11-beta-Hydroxylase