title: Protective effect of Na+ and K+ against inactivation of (Na+ + K+)-ATPase by high concentrations of 2-mercaptoethanol at high temperatures.
abstract: Purified dog kidney (Na+ + K+)-ATPase (EC 3.6.1.3) was inactivated with high concentrations of 2-mercaptoethanol at 50-55 degrees C. The inactivation was prevented by NaCl or KCl, with KCl being more effective than NaCl (the former ion being about one order more efficient under a typical set of experimental conditions). A disulfide bond in the beta-subunit of the enzyme protein was prevented from reductive cleavage by NaCl or KCl in accordance with protection of the enzyme activity. Choline chloride did not exert a significant protective effect over a similar concentration range. (Na+ + K+)-ATPase was also inactivated with high concentrations of 2-mercaptoethanol in the presence of low concentrations of dodecyl sulfate. This inactivation was also prevented by NaCl or KCl, with the latter being again more efficient than the former. These results indicate that Na+ and K+ bound to their respective ion-binding sites on the alpha-subunit exert a protective effect on a disulfide bond on the beta-subunit. This suggests some sort of interaction between the alpha- and the beta-subunits.
mesh_terms: D000818:Animals; D002712:Chlorides / Q000494:pharmacology; D002794:Choline / Q000494:pharmacology; D004220:Disulfides / Q000032:analysis; D004285:Dogs; D006358:Hot Temperature; D007668:Kidney / Q000201:enzymology*; D007700:Kinetics; D008094:Lithium / Q000494:pharmacology; D018021:Lithium Chloride; D008623:Mercaptoethanol / Q000494:pharmacology*; D011189:Potassium Chloride / Q000494:pharmacology*; D012965:Sodium Chloride / Q000494:pharmacology*; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors*
chemical_list: D002712:Chlorides; D004220:Disulfides; D012965:Sodium Chloride; D008623:Mercaptoethanol; D011189:Potassium Chloride; D008094:Lithium; D000254:Sodium-Potassium-Exchanging ATPase; D018021:Lithium Chloride; D002794:Choline