Purification of the insulin-like growth factor II (IGF-II) receptor from an IGF-II-producing cell line, and generation of an antibody which both immunoprecipitates and blocks the type 2 IGF receptor.
18,54-SF cells, which secrete rat insulin-like growth factor II (rIGF-II), have abundant type 2 IGF receptors. We have purified the type 2 receptor from these cells by solubilization of crude membranes in Triton X-100, followed by chromatography on agarose-immobilized rIGF-II. A partially purified receptor preparation, obtained by chromatography of solubilized membranes over wheat germ agglutinin, was used to immunize a rabbit. The antibody generated both immunoprecipitates the type 2 receptor, and specifically inhibits IGF-II binding to a variety of rat tissues, including 18,54-SF cells, BRL-3A cells and placenta. The presence of abundant type 2 receptors on an rIGF-II-secreting cell line is consistent with an autocrine role for IGF-II in select cells.
D000906:Antibodies / Q000276:immunology*; D002460:Cell Line; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000378:metabolism; D007700:Kinetics; D037102:Lectins / Q000378:metabolism; D017830:Octoxynol; D011092:Polyethylene Glycols; D011956:Receptors, Cell Surface / Q000276:immunology / Q000302:isolation & purification*; D017451:Receptors, Somatomedin; D012995:Solubility; D014909:Wheat Germ Agglutinins
D000906:Antibodies; D037102:Lectins; D011956:Receptors, Cell Surface; D017451:Receptors, Somatomedin; D014909:Wheat Germ Agglutinins; D011092:Polyethylene Glycols; D007334:Insulin-Like Growth Factor I; D017830:Octoxynol