Selective protection of cells against X-irradiation. Isoproterenol protects only those cells that possess beta-adrenoreceptors.
In mixed culture of Chinese hamster fibroblasts, clone 431, and transformed murine L fibroblasts, clone B-82, isoproterenol was found to protect only 431 cells against ionizing radiation. It was shown that 431 cells, in contrast to B-82 cells, possess beta-adrenoreceptors, and the radioprotective effect of isoproterenol can be realized only if this agent interacts with beta-adrenoreceptors coupled with the cAMP system. Since malignization often causes the disappearance of beta-adrenergic and other hormone receptors, the combined culturing and irradiation of the cells studied can be regarded as a model of the growth of malignant cells (B-82) among normal tissue cells (431 cells) under conditions of radiation therapy. A possibility of selective protection against radiation damage of normal tissue cells, with retention of the former radiosensitivity of tumor cells, is discussed.
D000262:Adenylyl Cyclases / Q000378:metabolism; D000527:Alprostadil / Q000494:pharmacology; D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D000242:Cyclic AMP / Q000378:metabolism; D004082:Dihydroalprenolol / Q000378:metabolism; D005347:Fibroblasts / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects*; D007545:Isoproterenol / Q000494:pharmacology*; D051379:Mice; D011433:Propranolol / Q000494:pharmacology; D011943:Receptors, Adrenergic, beta / Q000502:physiology*
D011943:Receptors, Adrenergic, beta; D004082:Dihydroalprenolol; D011433:Propranolol; D000242:Cyclic AMP; D000262:Adenylyl Cyclases; D000527:Alprostadil; D007545:Isoproterenol