DNA topoisomerase I from rat brain neurons.
The DNA topoisomerase I has been isolated from neurons of rat cerebral cortex. The most homogeneous fraction purified contains only one polypeptide of Mr approx. 100 000. The enzyme relaxes supercoiled DNA in the absence of ATP or Mg2+. The optimum monovalent cation concentration for the relaxation of superhelical DNA under conditions of DNA excess is found to be 175-200 mM. The neuron enzyme is similar to other mammalian type I DNA topoisomerases in that it links to the 3' ends of the broken DNA strands. Like calf thymus DNA topoisomerase I, the neuron topoisomerase can be selectively inhibited by poly(dG) but not by other homopolymerical deoxyribonucleotides.
D000818:Animals; D002467:Cell Nucleus / Q000201:enzymology; D002540:Cerebral Cortex / Q000201:enzymology*; D004264:DNA Topoisomerases, Type I / Q000302:isolation & purification* / Q000378:metabolism; D004278:DNA, Superhelical / Q000378:metabolism; D009474:Neurons / Q000201:enzymology*; D011068:Poly G / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D059004:Topoisomerase I Inhibitors
D004278:DNA, Superhelical; D059004:Topoisomerase I Inhibitors; D011068:Poly G; C026628:poly(dG); D004264:DNA Topoisomerases, Type I