A single radial hemolysis technique for the measurement of influenza virus antibody in cattle serum.
A single radial hemolysis technique (SRH) was used to measure swine influenza virus antibody in calf serum. Heating at 56 C for 60 minutes was necessary to prevent non-specific hemolysis. A significant association was found between the mean diameter of the hemolysis zone obtained with the SRH test and the geometric mean hemagglutination inhibition (HI) titer in sera of 5 calves inoculated with the virus after treatment with periodate (r = 0.92, P 0.01) and receptor destroying enzyme (r = 0.94, P 0.01). Although the SRH technique is not affected by the presence of non-specific inhibitors it is no more sensitive than the HI test. Some disadvantages of the technique included several biological variables difficult to control.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology; D006385:Hemagglutination Inhibition Tests; D006462:Hemolytic Plaque Technique; D009980:Influenza A virus / Q000276:immunology*; D009976:Orthomyxoviridae Infections / Q000276:immunology / Q000662:veterinary
D000914:Antibodies, Viral