Generation of radical anions from metronidazole, misonidazole and azathioprine by photoreduction in the presence of EDTA.
Ultraviolet irradiation of the nitroimidazole derivatives metronidazole, misonidazole, azathioprine and 1-methyl-4-nitroimidazole in aqueous solution with various reductants produced the respective nitro radical anions, as detected by electron spin resonance spectroscopy. The most effective reductant, yielding high concentrations of the radical anions, was EDTA at pH 10. NADH, NADPH, formaldehyde glutathione and methanol were also tested but were less efficient as reductants.
D001379:Azathioprine; D004492:Edetic Acid / Q000494:pharmacology*; D004578:Electron Spin Resonance Spectroscopy; D005609:Free Radicals; D008795:Metronidazole; D008920:Misonidazole; D010084:Oxidation-Reduction; D010777:Photochemistry; D011048:Polarography
D005609:Free Radicals; D008795:Metronidazole; D008920:Misonidazole; D004492:Edetic Acid; D001379:Azathioprine