Autoradiographic localization of benzodiazepine receptor binding in dissociated cultures of fetal mouse cerebral cortex.
Autoradiography utilizing photoaffinity labelling with [3H]flunitrazepam was used in living cultures of fetal mouse cerebral cortex in situ to localize benzodiazepine receptor binding sites. There was a predominant localization of silver grains over neurons; however, substantial labelling also occurred over nonneuronal background cells. Clonazepam (0.1 microM) and Ro 5-4864 (0.1 microM) displaced substantial numbers of silver grains over neurons and background cells, respectively. In addition, clonazepam displaced 58-68% of specific grains over background cells and Ro 5-4864 displaced 30% of grains over neurons, suggesting that multiple cell types in the CNS may participate in the neuropharmacologic actions of the benzodiazepines.
D000345:Affinity Labels; D000818:Animals; D001345:Autoradiography; D001570:Benzodiazepinones / Q000378:metabolism; D001667:Binding, Competitive; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000378:metabolism*; D002998:Clonazepam / Q000378:metabolism; D004622:Embryo, Mammalian; D005445:Flunitrazepam / Q000378:metabolism; D051379:Mice; D009474:Neurons / Q000378:metabolism; D010777:Photochemistry; D011963:Receptors, GABA-A / Q000378:metabolism*
D000345:Affinity Labels; D001570:Benzodiazepinones; D011963:Receptors, GABA-A; C028513:4'-chlorodiazepam; D002998:Clonazepam; D005445:Flunitrazepam