Opiate-like and adrenocorticotrophin-like materials in equine pancreas.
Equine pancreatic acetone powder was extracted with an acetone-water-HCl mixture. An acid acetone powder resulted by adding a copious volume of acetone to the extract. The powder was subjected to salt fractionation, gel filtration and chromatography on CM-cellulose. Steroidogenic activity, ACTH-like immunoreactivity and opiate receptor binding activity were distributed among the CM-cellulose chromatographic fractions derived from material unretarded as well as from material retarded on Sephadex G-25. The data indicates a separation of steroidogenic and opiate receptor binding activities, and the presence of opiate-like molecules with different affinities of binding to mu and delta opiate receptors.
D000324:Adrenocorticotropic Hormone / Q000302:isolation & purification / Q000378:metabolism*; D000818:Animals; D002266:Carboxymethylcellulose Sodium; D002845:Chromatography; D004723:Endorphins / Q000302:isolation & purification / Q000378:metabolism*; D006736:Horses / Q000378:metabolism; D008066:Lipolysis; D008970:Molecular Weight; D010179:Pancreas / Q000378:metabolism*; D011863:Radioimmunoassay; D011957:Receptors, Opioid / Q000378:metabolism
D004723:Endorphins; D011957:Receptors, Opioid; D000324:Adrenocorticotropic Hormone; D002266:Carboxymethylcellulose Sodium