Effect of triethyltin on enzyme activity in human adult and cord red cells.
Since organotin compounds represent an environmental health hazard, we determined the effect of triethyltin bromide (TTB) on red blood cell (RBC) enzyme activity. TTB produced a concentration-dependent inhibition of hexokinase and pyrimidine 5'-nucleotidase for both adult and cord RBC. D-Glucose, but not ATP or MgCl2, prevented the hexokinase inhibition by TTB. Glucose-6-phosphate dehydrogenase, adenylate kinase, and hypoxanthine-guanine phosphoribosyltransferase were also inhibited by TTB. Cord RBC enzymes were more resistant to the effects of TTB than were adult RBC enzymes. Although TTB is a potent inhibitor of hexokinase, physiologic concentrations of glucose appear to protect the RBC during clinical tin intoxication.
D015720:5'-Nucleotidase; D000328:Adult; D004912:Erythrocytes / Q000201:enzymology*; D005260:Female; D005312:Fetal Blood / Q000201:enzymology*; D005947:Glucose / Q000494:pharmacology; D005954:Glucosephosphate Dehydrogenase / Q000097:blood; D006593:Hexokinase / Q000097:blood; D006801:Humans; D007041:Hypoxanthine Phosphoribosyltransferase / Q000097:blood; D066298:In Vitro Techniques; D009708:Nucleotidases / Q000097:blood; D011247:Pregnancy; D014001:Tin / Q000494:pharmacology; D017971:Tin Compounds; D014220:Trialkyltin Compounds / Q000494:pharmacology*; D014267:Triethyltin Compounds / Q000494:pharmacology*
D017971:Tin Compounds; D014220:Trialkyltin Compounds; D014267:Triethyltin Compounds; C023599:stannous chloride; D014001:Tin; D005954:Glucosephosphate Dehydrogenase; D007041:Hypoxanthine Phosphoribosyltransferase; D006593:Hexokinase; D009708:Nucleotidases; D015720:5'-Nucleotidase; D005947:Glucose; C026405:bromotriethylstannane