Transposon tagging to detect a latent virus in Myxococcus xanthus.
Transposon mutagenesis of the bacterium Myxococcus xanthus with the transposon Tn5 revealed a special class of bacterial mutants that transduced the transposon through culture supernatant fluids. Virus-like particles copurified with transducing activity. Transposon tagging for detecting these virus-like particles may be generally useful in isolating endogenous viral agents capable of transferring genetic information between cells.
D001435:Bacteriophages / Q000032:analysis*; D002500:Centrifugation, Isopycnic; D004251:DNA Transposable Elements; D008854:Microscopy, Electron; D009154:Mutation; D009231:Myxococcales / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D014771:Virion / Q000032:analysis
D004251:DNA Transposable Elements