Formation of varicella-zoster virus antigens in infected Vero cells.
The formation of varicella-zoster (V-Z) virus-associated antigens was studied in V-Z virus-infected Vero cells by means of indirect immunofluorescence. Early antigen (EA) was first detected inside V-Z virus-infected Vero cells 4 to 6 hr after infection, whereas surface membrane antigen (SMA) was expressed on the outer surface of infected cells 2 to 3 hr later than EA, and intranuclear late antigen (LA) was detected several hours later than SMA antigen. EA expression was not inhibited by cytosine arabinoside (Ara-C) treatment, whereas LA formation was completely blocked by Ara-C. The presence of two components of SMA early SMA (ESMA) and late SMA (LSMA), was suggested by this difference in susceptibility to Ara-C. The formation of all viral antigens, EA, SMA, and LA, was blocked by inhibitors of RNA and protein synthesis.
D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D000956:Antigens, Viral / Q000032:analysis / Q000096:biosynthesis*; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D003561:Cytarabine / Q000494:pharmacology; D004247:DNA / Q000096:biosynthesis; D014645:Herpesvirus 3, Human / Q000276:immunology*; D007106:Immune Sera / Q000276:immunology; D011817:Rabbits
D000954:Antigens, Surface; D000956:Antigens, Viral; D007106:Immune Sera; D003561:Cytarabine; D004247:DNA