In vitro interferon production: induction in human leukocytes after exposure to cytomegalovirus antigens.
In vitro lymphocytes from healthy donors with crude (HCMV), heat inactivated (HCMVi) and infected human fetal fibroblasts (HCMVFF) were stimulated. In all the experiments peak titers of alfa Interferon (IFN) were present 24 h after exposure to antigens. On the other hand, at least after seven days, only lymphocytes of seropositive donors stimulated by HCMVFF and HCMV showed gamma IFN production. Our HCMVFF gave much higher IFN yields than free virus. Our data suggest that HCMVFF and HCMV are recognised by lymphocytes of HCMV specific memory and HCMVFF give additional and more reproducible information on HCMV specific cellular immunity.
D000956:Antigens, Viral / Q000276:immunology*; D003587:Cytomegalovirus / Q000276:immunology*; D005347:Fibroblasts; D006801:Humans; D007370:Interferon Type I / Q000096:biosynthesis*; D007371:Interferon-gamma / Q000096:biosynthesis*; D008214:Lymphocytes / Q000276:immunology / Q000378:metabolism*
D000956:Antigens, Viral; D007370:Interferon Type I; D007371:Interferon-gamma