title: Fine analysis of the active H5 gene chromatin of chicken erythroid cells at different stages of differentiation.
abstract: We have analyzed the chromatin structure of a region that encompasses 14.4 X 10(3) base-pairs of the chicken histone H5 locus in adult erythroid cells at different stages of maturation. Seven of eight major lineage-specific DNase I-hypersensitive sites, some of which show complex substructure, were found in the flanking regions of the gene. The hypersensitivity of some of these sites is modulated during erythrocyte maturation in a way that parallels the transcriptional activity of the gene. DNase I, micrococcal nuclease, and S1 nuclease recognize the same regions, which differ from those cleaved by S1 on supercoiled plasmid DNA. This suggests that hypersensitivity of DNA in chromatin reflects a greater accessibility of the DNA rather than its altered conformation. The DNA sequence of some of the DNase I target sites contains repeated motifs, (T-C-C-C)2, (T-C-C)2, (T-G-G-G-G)2, which are found in the hypersensitive sites of other genes. Detailed analysis across sections of the H5 gene and flanking sequences revealed differences in the DNase I sensitivity of the different regions examined. Notably, the first one-third of the gene is more sensitive than the rest. The sequences downstream from the region where most RNA polymerases terminate transcription were found to be the most resistant.
mesh_terms: D000818:Animals; D001483:Base Sequence; D002454:Cell Differentiation; D002478:Cells, Cultured; D002645:Chickens; D002843:Chromatin / Q000235:genetics / Q000648:ultrastructure*; D002874:Chromosome Mapping; D004247:DNA / Q000378:metabolism; D003850:Deoxyribonuclease I / Q000494:pharmacology; D004720:Endonucleases / Q000494:pharmacology; D004912:Erythrocytes / Q000254:growth & development*; D005796:Genes / Q000187:drug effects; D006657:Histones / Q000235:genetics*; D008836:Micrococcal Nuclease / Q000494:pharmacology; D015719:Single-Strand Specific DNA and RNA Endonucleases
chemical_list: D002843:Chromatin; D006657:Histones; D004247:DNA; D004720:Endonucleases; D003850:Deoxyribonuclease I; D015719:Single-Strand Specific DNA and RNA Endonucleases; D008836:Micrococcal Nuclease