[The role of calmodulin (delta-subunit) in the activation of phosphorylase kinase from rabbit skeletal muscles].
A comparative study on the structure of nonactivated and activated forms of phosphorylase kinase was carried out. The enzyme was activated by incubation in alkaline medium (pH 8.5), by phosphorylation with cAMP-dependent protein kinase and by limited proteolysis. The comparative analysis was based on the use of hydrophobic chromatography on phenyl-sepharose and electrophoresis in polyacrylamide gel density gradient. Activation of the enzyme was accompanied by separation of a low molecular weight component (Mr about 17 000). Using chromatography on phenyl-sepharose, this low molecular weight protein was obtained in a homogeneous state. It was found that the properties of the protein are close to those of calmodulin. The presence of calmodulin in phosphorylase kinase preparations was judged upon by the activation of the calmodulin-dependent form of phosphodiesterase. The boiled and subtilisin-treated kinase activates phosphodiesterase in the same way as does bovine brain calmodulin. The experimental results suggest that the delta-subunit is a protein inhibitor of the enzyme.
D000818:Animals; D001923:Brain Chemistry; D002147:Calmodulin / Q000302:isolation & purification* / Q000378:metabolism; D002384:Catalysis; D002417:Cattle; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation; D066298:In Vitro Techniques; D008970:Molecular Weight; D009132:Muscles / Q000201:enzymology*; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism; D010764:Phosphorylase Kinase / Q000378:metabolism*; D011817:Rabbits
D002147:Calmodulin; D010764:Phosphorylase Kinase; D010727:Phosphoric Diester Hydrolases
Rol' kal'modulina (delta-sub''edinitsy) v protsesse aktivatsii kinazy fosforilazy iz skeletnykh myshts krolika.