title: Viral DNA sequences in human cytomegalovirus transformed hamster cell line at low passage levels.
abstract: The characteristics of a human cytomegalovirus (HCMV) transformed syrian hamster cell line (87-TRH-5) were examined. The cytomegalovirus DNA (HCMV-DNA) labelled in vitro by nick translation was used as a probe to detect viral DNA sequences in the 87-TRH-5 line. Cytomegalovirus specified DNA sequences were detected in cells examined up to passage 58, but were undetectable at higher passage levels. Six clones were derived from the 28th passage of 87-TRH-5 cells and examined for HCMV-DNA sequences. Various amounts of HCMV-DNA were found. A tumour induced by passage 28 cells contained no detectable HCMV-DNA sequences.
mesh_terms: D000818:Animals; D002460:Cell Line; D002472:Cell Transformation, Viral; D006224:Cricetinae; D003587:Cytomegalovirus / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification*; D004622:Embryo, Mammalian; D006801:Humans; D007700:Kinetics; D008168:Lung / Q000196:embryology; D008647:Mesocricetus; D009693:Nucleic Acid Hybridization
chemical_list: D004279:DNA, Viral