Effects of fluoride on retinal rod outer segment cGMP phosphodiesterase and G-protein.
The effects of fluoride on ROS phosphodiesterase and G-protein have been studied using membrane-free extracts. When G-protein was present NaF, at millimolar concentrations, stimulated PDE activity however, in a G-protein free extract, cGMP hydrolysis was inhibited by high fluoride concentrations. Fluoride was also found to profoundly inhibit the ability of G-protein to bind a GTP analogue, GTP gamma S, both in the presence and absence of rhodopsin. Aluminium greatly modified these effects of fluoride on PDE and G-protein. The possibility that fluoride activates PDE through its effect on G-protein is discussed.
D015106:3',5'-Cyclic-GMP Phosphodiesterases / Q000378:metabolism*; D000535:Aluminum / Q000494:pharmacology; D000818:Animals; D002417:Cattle; D006152:Cyclic GMP / Q000378:metabolism; D005136:Eye Proteins / Q000378:metabolism*; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D006160:Guanosine Triphosphate / Q000031:analogs & derivatives / Q000378:metabolism; D020962:Heterotrimeric GTP-Binding Proteins; D010786:Photoreceptor Cells / Q000378:metabolism*; D012243:Rhodopsin / Q000494:pharmacology; D012374:Rod Cell Outer Segment / Q000187:drug effects / Q000378:metabolism*; D012969:Sodium Fluoride / Q000494:pharmacology*; D013873:Thionucleotides / Q000378:metabolism; D015293:Transducin
D005136:Eye Proteins; C034809:GNAT1 protein, human; D013873:Thionucleotides; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D006160:Guanosine Triphosphate; D012969:Sodium Fluoride; D012243:Rhodopsin; D000535:Aluminum; D015106:3',5'-Cyclic-GMP Phosphodiesterases; D020962:Heterotrimeric GTP-Binding Proteins; D015293:Transducin; D006152:Cyclic GMP