Lycorine: a eukaryotic termination inhibitor?
The effect of the alkaloid lycorine on viral protein synthesis was studied in poliovirus-infected HeLa cells. The incorporation of [3H]leucine was inhibited by lycorine in a dose-dependent way, although lycorine never completely abolished translation. Using polyacrylamide gel electrophoresis, the viral proteins were identified as derived from the P1 (5' terminal), P2 (middle), or P3 (3' terminal) region of the poliovirus translation unit. The residual labeling of viral proteins in the presence of lycorine was mainly due to synthesis of P1 proteins and slightly less to P2 proteins, while virtually no P3-derived proteins were made. It is suggested that lycorine may act at the level of termination.
D047151:Amaryllidaceae Alkaloids; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D006367:HeLa Cells; D006801:Humans; D007930:Leucine / Q000378:metabolism; D010617:Phenanthridines / Q000494:pharmacology*; D017955:Poliovirus; D014764:Viral Proteins / Q000096:biosynthesis*
D047151:Amaryllidaceae Alkaloids; D010617:Phenanthridines; D014764:Viral Proteins; D007930:Leucine; C015330:lycorine