A continuous spectrophotometric assay for pyrimidine-5'-nucleotidase.
A simple method is presented for the determination of pyrimidine-5'-nucleotidase activity using a continuous spectrophotometric assay system. Activity is determined by measuring inorganic phosphate generation using a linked indicator system that produces uric acid in the presence of inosine, purine nucleoside phosphorylase, and xanthine oxidase. This method has several advantages over any of the methods currently in use.
D015720:5'-Nucleotidase; D004912:Erythrocytes / Q000201:enzymology*; D006461:Hemolysis; D006801:Humans; D007700:Kinetics; D009708:Nucleotidases / Q000097:blood*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
D009708:Nucleotidases; D015720:5'-Nucleotidase