[Immunohistochemical study on trophoblast subpopulations in normal pregnancy and trophoblastic disease].
Trophoblast subpopulations were analyzed by their antigen expressions in normal pregnancy and trophoblastic disease. Samples were taken from uteri which were removed surgically. Frozen sections were stained by an avidin-biotin-peroxidase complex method, using monoclonal antibodies to HLA antigens and anti-Trop 1 antibody. Troma 1, a rat monoclonal antibody, was used for the identification of trophoblasts. The results were as follows: Troma 1 reacted with all trophoblasts specifically and could be used as a trophoblast marker in sections. HLA-A,B,C was negative on syncytiotrophoblast and villous cytotrophoblast but positive on nonvillous cytotrophoblast in normal pregnancy and hydatidiform mole. Some choriocarcinoma cells were positive for HLA-A,B,C and they were intermediate to large cytotrophoblast-like cells. No trophoblasts were positive for HLA-DR. Trop 1 was positive on villous cytotrophoblast in normal pregnancy and hydatidiform mole. Some choriocarcinoma cells were positive for Trop 1 and they were exclusively negative for HLA-A,B,C. From these immunohistochemical findings, trophoblasts of normal pregnancy and hydatidiform mole were divided into syncytiotrophoblast, villous cytotrophoblast, and nonvillous cytotrophoblast. Choriocarcinoma cells were classified into syncytiotrophoblast-like cells and three kinds of cytotrophoblast-like cells. They were analogous to those of normal pregnancy and hydatidiform mole to some extent, but a choriocarcinoma-specific subpopulation which was negative for HLA-A,B,C, HLA-DR, and Trop 1 was recognized.
D000911:Antibodies, Monoclonal / Q000276:immunology; D002454:Cell Differentiation; D002471:Cell Transformation, Neoplastic; D005260:Female; D006680:HLA Antigens / Q000032:analysis; D006801:Humans; D007124:Immunoenzyme Techniques; D011247:Pregnancy; D014328:Trophoblastic Neoplasms / Q000276:immunology* / Q000473:pathology; D014327:Trophoblasts / Q000166:cytology / Q000276:immunology*; D014594:Uterine Neoplasms / Q000276:immunology* / Q000473:pathology
D000911:Antibodies, Monoclonal; D006680:HLA Antigens