Isolation and subregional mapping of an arbitrary cloned probe detecting a common RFLP on human chromosome 2.
In the course of studies on restriction fragment length polymorphisms (RFLPs) being conducted in our laboratory, several single-copy cloned probes have been generated from specific human chromosomes using murine-human hybrid cell libraries. The following describes the isolation and subregional localization of an arbitrary single-copy cloned probe for human chromosome 2. This probe, designated pXG-18, has detected a common TaqI polymorphism in addition to two other RFLPs using the restriction enzymes MspI and HindIII. This sequence maps to the interval q32-q36 of chromosome 2, a region of the human genome to which very few markers have been assigned.
D000818:Animals; D002871:Chromosome Banding; D002874:Chromosome Mapping; D002900:Chromosomes, Human, 1-3; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004587:Electrophoresis, Agar Gel; D008040:Genetic Linkage; D006801:Humans; D006822:Hybrid Cells; D051379:Mice; D009693:Nucleic Acid Hybridization; D010375:Pedigree; D011110:Polymorphism, Genetic
D004262:DNA Restriction Enzymes