Purification of SV40 T-antigen by SV40 DNA-sepharose affinity chromatography.
T-antigen from SV40-infected BSC-1 cells was purified approximately 30,000 fold using a rapid purification procedure consisting of ammonium sulfate fractionation followed by chromatography on hydroxylapatite, blue-sepharose, and SV40 DNA-sepharose. The SV40 DNA-sepharose was optimized for the binding of T-antigen by the covalent attachment of the SV40 DNA at its BamHI site to cyanogen bromide activated sepharose. The most highly purified T-antigen appeared as a single polypeptide of 94 K daltons by polyacrylamide gel electrophoresis.
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000957:Antigens, Viral, Tumor / Q000302:isolation & purification*; D002846:Chromatography, Affinity / Q000379:methods; D004279:DNA, Viral / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000302:isolation & purification* / Q000378:metabolism; D013539:Simian virus 40 / Q000032:analysis* / Q000201:enzymology / Q000276:immunology
D000957:Antigens, Viral, Tumor; D004279:DNA, Viral; D004268:DNA-Binding Proteins; D000251:Adenosine Triphosphatases