Structure of the O-chain polysaccharide of the phenol-phase soluble lipopolysaccharide of Escherichia coli 0:157:H7.
The phenol-phase soluble lipopolysaccharide isolated from Escherichia coli 0:157 by the hot phenol-water extraction procedure was shown by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, periodate oxidation, methylation, and 13C and 1H nuclear magnetic resonance studies to be an unbranched linear polysaccharide with a tetrasaccharide repeating unit having the structure: (formula; see text) The serological cross-reactivity of E. coli 0:157 with Brucella abortus, Yersinia enterocolitica (serotype 0:9), group N Salmonella, and some other E. coli species can be related immunochemically to the presence of 1,2-glycosylated N-acylated 4-amino-4, 6-dideoxy-alpha-D-mannopyranosyl residues in the O-chains of their respective lipopolysaccharides.
D002236:Carbohydrate Conformation; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000032:analysis*; D005643:Fucose; D005947:Glucose; D008070:Lipopolysaccharides / Q000302:isolation & purification; D008745:Methylation; D009844:Oligosaccharides; D010084:Oxidation-Reduction; D010504:Periodic Acid; D010636:Phenols; D012995:Solubility
D008070:Lipopolysaccharides; D009844:Oligosaccharides; D010636:Phenols; D010504:Periodic Acid; D005643:Fucose; C009288:metaperiodate; D005947:Glucose