Repetitive DNA as a tool for the identification and comparison of nematode variants: application to Trichinella isolates.
DNA prepared from four isolates of Trichinella was compared by genomic DNA cross-hybridisation, by electrophoresis following restriction endonuclease digestion and by hybridisation studies using a cloned repetitive DNA sequence from T. spiralis. The DNA from T. spiralis, T. nelsoni and T. pseudospiralis isolates was distinct and the interrelationships of these isolates were inferred. In contrast to previous work on T. nativa and T. spiralis, our work suggests that these two isolates are very similar.
D000818:Animals; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis*; D004262:DNA Restriction Enzymes; D004587:Electrophoresis, Agar Gel; D005796:Genes; D009693:Nucleic Acid Hybridization; D012091:Repetitive Sequences, Nucleic Acid; D012689:Sequence Homology, Nucleic Acid; D014234:Trichinella / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification
D004247:DNA; D004262:DNA Restriction Enzymes