title: Effects of phosphatase inhibitors on nuclease activity.
abstract: Nucleases such as DNase I, which selectively digest chromatin, are inhibited by several commonly used phosphatase inhibitors including sodium bisulfite. Two inhibitors, sodium arsenate and fructose-1,6-diphosphate, did not significantly inhibit nuclease action. Two other effective phosphatase inhibitors, p-chloromercuriphenyl sulfonate and 5,5'-dithiobis(2-nitrobenzoate), can be used during nuclei isolation and then washed out of nuclei before nuclease digestion. Using this procedure, 1mM p-chloromercuriphenyl sulfonate is as effective as 50mM bisulfite in retaining the phosphatase-sensitive mitotic phosphorylations of histones H1 and H3.
mesh_terms: D002731:4-Chloromercuribenzenesulfonate / Q000494:pharmacology*; D000469:Alkaline Phosphatase / Q000037:antagonists & inhibitors*; D000818:Animals; D001149:Arsenates / Q000494:pharmacology*; D001151:Arsenic / Q000494:pharmacology*; D002460:Cell Line; D002843:Chromatin / Q000648:ultrastructure; D006224:Cricetinae; D003412:Cricetulus; D003850:Deoxyribonuclease I / Q000037:antagonists & inhibitors*; D004228:Dithionitrobenzoic Acid / Q000494:pharmacology*; D005260:Female; D005635:Fructosediphosphates / Q000494:pharmacology*; D006598:Hexosediphosphates / Q000494:pharmacology*; D007700:Kinetics; D051379:Mice; D009579:Nitrobenzoates / Q000494:pharmacology*; D010053:Ovary; D010663:Phenylmercury Compounds / Q000494:pharmacology*
chemical_list: D001149:Arsenates; D002843:Chromatin; D005635:Fructosediphosphates; D006598:Hexosediphosphates; D009579:Nitrobenzoates; D010663:Phenylmercury Compounds; D002731:4-Chloromercuribenzenesulfonate; C009277:sodium arsenate; D004228:Dithionitrobenzoic Acid; D003850:Deoxyribonuclease I; D000469:Alkaline Phosphatase; C029063:fructose-1,6-diphosphate; D001151:Arsenic