Expression of a bacterial repair gene in mammalian cells.
The coding sequence of the uvrA gene from Escherichia coli has been fused to the early promoter, enhancer and origin of replication of the simian virus SV40, and was supplemented with splicing and polyadenylation sites arising from the same virus. Introduction of this hybrid gene into simian cos-1 cells results in the synthesis of a full length UvrA protein (114 kD) which has retained its ability to bind to single-stranded DNA.
D000818:Animals; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics; D002460:Cell Line; D004260:DNA Repair; D004261:DNA Replication; D004274:DNA, Recombinant; D004742:Enhancer Elements, Genetic; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D005809:Genes, Regulator; D005814:Genes, Viral; D010957:Plasmids; D011401:Promoter Regions, Genetic; D013539:Simian virus 40 / Q000235:genetics*; D014162:Transfection
D001426:Bacterial Proteins; D004274:DNA, Recombinant