[Determining the motility of glycoprotein oligosaccharides from lymphocyte membranes using the spin label method].
Splenocyte glycoproteins solubilized by papain were purified on lectyl-lectin Sepharose 4B. Glycoproteins eluted from lectin were spin-labeled at carbohydrates. Quantitative evaluation of ESR spectra of spin-labeled glycoproteins pointed to strong restriction of reorientation of the spin label bound to oligosaccharides. Calculated correlation time of relaxing volume tagged with the spin label was equal to the molecular weight about 6000-7000 dalton. This value suggests the existence of flexibility of the glycoproteins studied.
D000818:Animals; D002462:Cell Membrane / Q000032:analysis; D004578:Electron Spin Resonance Spectroscopy; D006023:Glycoproteins / Q000032:analysis*; D008214:Lymphocytes / Q000032:analysis*; D008565:Membrane Proteins / Q000032:analysis; D051379:Mice; D009844:Oligosaccharides / Q000032:analysis*; D013113:Spin Labels
D006023:Glycoproteins; D008565:Membrane Proteins; D009844:Oligosaccharides; D013113:Spin Labels
Opredelenie podvizhnosti oligosakharidov glikoproteinov iz membran limfotsitov metodom spinovoĭ metki.