Influence of gyrA mutation on expression of Erwinia chrysanthemi clb genes cloned in Escherichia coli.
Erwinia chrysanthemi clb genes cloned into Nals Escherichia coli allowed growth on cellobiose, arbutin, or salicin. In contrast, Nalr isogenic strains grew only on cellobiose. It is proposed that expression of cloned E. chrysanthemi clb genes is reduced by the E. coli chromosomal gyrA (Nalr) mutation, resulting in apparent segregation of the Clb and Arb Sal characters.
D002475:Cellobiose / Q000235:genetics*; D003001:Cloning, Molecular; D004250:DNA Topoisomerases, Type II / Q000235:genetics*; D004187:Disaccharides / Q000235:genetics*; D004885:Erwinia / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D009154:Mutation; D010641:Phenotype; D014161:Transduction, Genetic
D004187:Disaccharides; D002475:Cellobiose; D004250:DNA Topoisomerases, Type II