[Effects of insulin and somatomedin C on the function of cultured Leydig cells].
Porcine cultured Leydig cells (LC) lose hCG receptors and hCG responsiveness (cAMP and testosterone) when they are cultured for three days in a defined medium without insulin or somatomedin C (Sm-C) (Insulin-like growth factor I). In the presence of insulin (50 ng/ml) or of Sm-C (10 ng/ml) the loss of the hCG receptor number and the decreased cAMP response to hCG were prevented, but the steroidogenic response to hCG was only partially prevented. This parameter became normal when cells were pretreated with either Sm-C (10 ng/ml) plus insulin (50 ng/ml) or with insulin alone at high concentrations (5 micrograms/ml). These results indicate that both Sm-C and insulin acting through their own receptors increase Leydig cell steroidogenic capacity by increasing hCG receptor number and improving some step beyond cAMP formation.
D000818:Animals; D002478:Cells, Cultured; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000096:biosynthesis; D007328:Insulin / Q000494:pharmacology*; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D007985:Leydig Cells / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D011974:Receptors, LH / Q000378:metabolism; D013002:Somatomedins / Q000494:pharmacology*; D013552:Swine; D013739:Testosterone / Q000096:biosynthesis
D006063:Chorionic Gonadotropin; D007328:Insulin; D011974:Receptors, LH; D013002:Somatomedins; D005576:Colforsin; D013739:Testosterone; D007334:Insulin-Like Growth Factor I; D000242:Cyclic AMP
Effets de l'insuline et de la somatomédine C sur la fonction des cellules de Leydig en culture.