Functional reconstitution of class II major histocompatibility complex molecules, I-A alpha k and I-A beta k.
Genomic DNA fragments carrying the genes, I-A alpha k and I-A beta k, encoding the class II major histocompatibility complex (MHC) molecule I-Ak were inserted into a transducing vector pRSVneo. This vector was introduced into a B lymphoma line expressing the class II molecules (H-2d). The resultant transformants expressed I-Ak molecules on the membrane surface. These transformants were capable of presenting antigen to I-Ak restricted helper T (Th) cells and were susceptible to lysis by class II specific killer T cells. The vector pR-A alpha kA beta k which contains both I-A alpha k and I-A beta k genes will provide a useful tool to analyze function of Ia molecules in cellular interaction, recognition, regulations and stimulation.
D000818:Animals; D002460:Cell Line; D004251:DNA Transposable Elements; D005822:Genetic Vectors; D000949:Histocompatibility Antigens Class II; D008285:Major Histocompatibility Complex; D051379:Mice; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA; D006377:T-Lymphocytes, Helper-Inducer; D014161:Transduction, Genetic
D004251:DNA Transposable Elements; D000949:Histocompatibility Antigens Class II