Microtubule disassembly increases the number of opioid receptor binding sites in rat cerebrum membranes.
The role of microtubules in opioid receptor binding was studied by using microtubule assembly inhibitors. Preincubation of rat cerebrum membranes with podophyllotoxin or colchicine provoked a marked increase in the number of binding sites as judged by [3H]-naloxone, [3H]-morphine and [3H]-D-Ala2-Leu5-enkephalin binding experiments. These results indicate microtubule involvement in regulation of opioid receptor expression.
D000818:Animals; D001921:Brain / Q000378:metabolism* / Q000648:ultrastructure; D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure; D004090:Dihydromorphine / Q000378:metabolism; D004744:Enkephalin, Methionine / Q000031:analogs & derivatives / Q000378:metabolism; D007700:Kinetics; D007981:Levorphanol / Q000494:pharmacology; D008870:Microtubules / Q000378:metabolism* / Q000648:ultrastructure; D009270:Naloxone / Q000378:metabolism / Q000494:pharmacology; D011034:Podophyllotoxin / Q000494:pharmacology; D051381:Rats; D011957:Receptors, Opioid / Q000187:drug effects / Q000378:metabolism*
D011957:Receptors, Opioid; D007981:Levorphanol; D009270:Naloxone; D004744:Enkephalin, Methionine; C022118:enkephalinamide-Met, Ala(2)-; D004090:Dihydromorphine; D011034:Podophyllotoxin