Effect of using heat-inactivated serum with the Abbott human T-cell lymphotropic virus type III antibody test.
The Abbott enzyme immunoassay (Abbott Laboratories, North Chicago, Ill.) for human T-cell lymphotropic virus type III (HTLV-III) antibody was evaluated to determine the effect of using heat-inactivated (56 degrees C for 30 min) serum as the sample. Each of 58 nonreactive serum samples gave a higher A492 value when tested after heat inactivation. Ten of the samples became reactive after heating. Heat-inactivated serum should not be used in the current Abbott HTLV-III antibody test, because this can cause false-positive results.
D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology / Q000635:transmission; D000914:Antibodies, Viral / Q000032:analysis*; D001769:Blood; D017977:Deltaretrovirus / Q000276:immunology*; D005069:Evaluation Studies as Topic; D005189:False Positive Reactions; D015483:HIV Antibodies; D006358:Hot Temperature; D006801:Humans; D007124:Immunoenzyme Techniques
D000914:Antibodies, Viral; D015483:HIV Antibodies