title: Polypeptides encoded by varicella-zoster virus unique short sequences.
abstract: The SalI-I and K DNA fragments which lie within the unique short sequences (Us) and contain a portion of the inverted repeat sequences (IRs/TRs) of varicella-zoster virus (VZV) DNA were cloned in an in vitro transcription vector system (pGEM-2). RNA was transcribed from both strands, translated in vitro and analyzed by SDS-PAGE. The results showed that SalI-I and K each codes for three primary translation products. Polypeptides with Mrs of 19,000 (19K), 47K, 93K/90K are encoded by SalI-I and polypeptides of 12K, 19K, and 50K are encoded by SalI-K. These results are consistent with the predicted genetic expression of the VZV SalI-I and SalI-K DNA fragments.
mesh_terms: D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005814:Genes, Viral; D014645:Herpesvirus 3, Human / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D010455:Peptides / Q000032:analysis / Q000235:genetics; D014176:Protein Biosynthesis; D012367:RNA, Viral / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000032:analysis / Q000235:genetics*
chemical_list: D004279:DNA, Viral; D010455:Peptides; D012367:RNA, Viral; D014764:Viral Proteins