Partial dependency of 1-oleoyl-2-acetyl-glycerol-induced superoxide production by human neutrophils on calcium ions and cytochalasin B.
Superoxide production by neutrophils induced by 1-oleoyl-2-acetyl-sn-glycerol at concentrations below 100 microM was enhanced by extracellular calcium ions, while that of phorbol myristate acetate was unaffected. Verapamil, a calcium-channel blocker, more effectively inhibited the superoxide production induced by 1-oleoyl-2-acetyl-sn-glycerol than that of phorbol myristate acetate. Cytochalasin B at 5 micrograms/ml significantly potentiated superoxide production by 1-oleoyl-2-acetyl-sn-glycerol at concentrations below 100 microM, but not that of phorbol myristate acetate. It is suggested that neutrophil activation induced by the former have different features from that of the latter.
D002118:Calcium / Q000494:pharmacology*; D003571:Cytochalasin B / Q000494:pharmacology*; D004075:Diglycerides / Q000494:pharmacology*; D005707:Gallic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D005989:Glycerides / Q000494:pharmacology*; D006801:Humans; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014700:Verapamil / Q000494:pharmacology
D004075:Diglycerides; D005989:Glycerides; D013481:Superoxides; D003571:Cytochalasin B; C006014:8-(N,N-diethylamino)octyl-3,4,5-trimethoxybenzoate; D005707:Gallic Acid; C038237:1-oleoyl-2-acetylglycerol; D014700:Verapamil; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium