Intracellular Ca2+ requirements for zymosan-stimulated phosphoinositide hydrolysis in mouse peritoneal macrophages.
The phosphatidylinositol cycle has been demonstrated to be involved in the control of Ca2+ cytosolic levels in several cellular types. The Ca2+ requirements of phospholipase C activity and the described stimulation of phosphoinositide hydrolysis by Ca2+ ionophores make unclear the relationship between phosphatidylinositol cycle and Ca2+ mobilization. The results reported here suggest that intracellular Ca2+ is necessary for zymosan-stimulated phospholipase C activation in macrophages.
D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D002396:Catechols / Q000494:pharmacology; D004789:Enzyme Activation; D007213:Indomethacin / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D009637:Masoprocol; D051379:Mice; D010716:Phosphatidylinositols / Q000378:metabolism*; D013997:Time Factors; D010738:Type C Phospholipases / Q000378:metabolism; D015054:Zymosan / Q000494:pharmacology*
D001095:Arachidonic Acids; D002396:Catechols; D010716:Phosphatidylinositols; D016718:Arachidonic Acid; D000001:Calcimycin; D009637:Masoprocol; D015054:Zymosan; D010738:Type C Phospholipases; D002118:Calcium; D007213:Indomethacin