Polyproteins containing a domain encoded by the V-SKI oncogene are located in the nuclei of SKV-transformed cells.
SKV-transformed nonproducer clones were isolated from infected quail and chicken embryo cells. Analysis of intracellular viral RNAs by the Northern technique revealed that each clone contained a single SKV genome (either 5.7 or 8.9 kb) but no genome of the helper virus. Analysis of intracellular viral proteins containing gag determinants revealed that each clone contained a single species of either 55, 110, or 125 kDa. The intracellular location of these proteins was determined by indirect immunofluorescence employing either monoclonal antibodies (anti-p19gag) or conventional antiserum against gag proteins. All three of the SKV-specific proteins were localized to the nuclei of the transformed cells.
D000818:Animals; D001354:Avian Leukosis Virus / Q000235:genetics / Q000502:physiology*; D002467:Cell Nucleus / Q000032:analysis*; D002471:Cell Transformation, Neoplastic; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D002642:Chick Embryo; D002999:Clone Cells; D005455:Fluorescent Antibody Technique; D009856:Oncogene Proteins, Viral / Q000032:analysis*; D009857:Oncogenes; D011784:Quail
D009856:Oncogene Proteins, Viral