[Use of permeabilized cells in the study of inhibitory products of herpes virus replication].
Cells made permeable by exposure to lysolecithin following infection by HSV-1 synthesize DNA (in greater amounts than non-infected cells) in the presence of the four deoxyribonucleoside-triphosphates (dNTPs) : dATP, dCTP, dGTP, and dTTP. DNA synthesis also occurs if dTTP is replaced by dT or dTMP, indicating activity of enzymes such as thymidine kinase, thymidylate kinase, deoxyribonucleoside-diphosphate kinase and ADN polymerase. Examination of DNA synthesis in permeabilized cells enables detection of antiviral activity of agents incapable of penetrating into intact cells and therefore ineffective in cell cultures. No detectable protein-tyrosine kinase activity was found in HSV-1 infected cells.
D000998:Antiviral Agents / Q000494:pharmacology*; D002460:Cell Line; D002463:Cell Membrane Permeability / Q000187:drug effects; D003565:Cytidine Diphosphate / Q000378:metabolism; D004279:DNA, Viral / Q000096:biosynthesis; D003854:Deoxyribonucleotides / Q000378:metabolism; D005260:Female; D006801:Humans; D008244:Lysophosphatidylcholines / Q000494:pharmacology; D010920:Placenta; D011247:Pregnancy; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D018139:Simplexvirus / Q000502:physiology*; D013936:Thymidine / Q000378:metabolism; D013938:Thymidine Monophosphate / Q000378:metabolism; D013942:Thymine Nucleotides / Q000378:metabolism; D014779:Virus Replication / Q000187:drug effects*
D000998:Antiviral Agents; D004279:DNA, Viral; D003854:Deoxyribonucleotides; D008244:Lysophosphatidylcholines; D013942:Thymine Nucleotides; D013938:Thymidine Monophosphate; D003565:Cytidine Diphosphate; D011505:Protein-Tyrosine Kinases; C024157:thymidine 5'-triphosphate; D013936:Thymidine
Utilisation de cellules perméabilisées dans l'étude de produits inhibiteurs de la replication du virus de l'herpès.