Detection of cytomegalovirus antibody by enzyme immunoassay and lack of evidence for an effect resulting from strain heterogeneity.
A total of 259 serum samples from random blood donors were assayed by an enzyme immunoassay for antibody to cytomegalovirus (CMV) with antigens prepared from eight genetically unrelated clinical isolates of CMV and the laboratory-adapted strains AD169 and Towne. A total of 144 serum samples were reactive against all 10 strains, and 115 serum samples lacked immunoglobulin against all 10 strains. The results support the continued use of the AD169 strain of human CMV as the reference antigen for CMV serologic testing.
D000914:Antibodies, Viral / Q000032:analysis*; D000956:Antigens, Viral / Q000276:immunology; D001782:Blood Donors; D003587:Cytomegalovirus / Q000276:immunology*; D006801:Humans; D007124:Immunoenzyme Techniques
D000914:Antibodies, Viral; D000956:Antigens, Viral