Electron microscopic evidence for the cruciform structure in intracellular SV40 DNA.
The conformation of intracellular SV40 DNA during lytic infection of CV-1 cells was studied by the psoralen crosslinking technique. Analysis of the crosslinked SV40 DNA in the electron microscope revealed a rare population (0.1%) with a cruciform structure at coordinates 0.62 +/- 0.05 or at 0.37 +/- 0.05 of the SV40 genome. The implication of this observation in relation to SV40 DNA replication is discussed.
D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000302:isolation & purification*; D005814:Genes, Viral; D008854:Microscopy, Electron; D009690:Nucleic Acid Conformation; D013539:Simian virus 40 / Q000235:genetics*
D004279:DNA, Viral; D004262:DNA Restriction Enzymes