Induction of a deoxyuridine triphosphate nucleotidohydrolase activity in Epstein-Barr virus-infected cells.
Superinfection of Raji cells with Epstein-Barr virus (EBV) or chemical induction of HR-1 cells with 12-O-tetradecanoyl-phorbol-13-acetate (TPA) results in the induction of a deoxyuridine triphosphate nucleotidohydrolase (dUTPase) which is not observed in mock-treated cells or TPA-treated EBV genome-negative BJAB cells. The EBV-induced dUTPase could be distinguished from the host dUTPase based upon differences in their migration in polyacrylamide gels and sensitivity to the 5-mercurithioguanosine derivitive of dUTP. The expression of the EBV-specified dUTPase is prevented by phosphonoacetic acid indicating that its expression is dependent upon EBV-DNA replication.
D000998:Antiviral Agents / Q000494:pharmacology; D002051:Burkitt Lymphoma; D002460:Cell Line; D002472:Cell Transformation, Viral; D004261:DNA Replication; D004790:Enzyme Induction; D017245:Foscarnet; D004854:Herpesvirus 4, Human / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D006801:Humans; D007700:Kinetics; D010746:Phosphonoacetic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D011755:Pyrophosphatases / Q000096:biosynthesis*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014779:Virus Replication
D000998:Antiviral Agents; D017245:Foscarnet; D011755:Pyrophosphatases; C020020:dUTP pyrophosphatase; D010746:Phosphonoacetic Acid; D013755:Tetradecanoylphorbol Acetate