Protein blot analysis of virus receptors: identification and characterization of the Sendai virus receptor.
Receptors for Sendai virions in human erythrocyte ghost membranes were identified by virus overlay of protein blots. Among the various erythrocyte polypeptides, only glycophorin was able to bind Sendai virions effectively. The detection of Sendai virions bound to glycophorin was accomplished either by employing anti-Sendai virus antibodies or by autoradiography, when 125I-labeled Sendai virions were used. The binding activity was associated with the viral hemagglutinin/neuraminidase (HN) glycoprotein, as inferred from the observation that the binding pattern of purified HN glycoprotein to human erythrocyte membranes was identical to that of intact Sendai virions. No binding was observed when blots, containing either human erythrocyte membranes or purified glycophorin, were probed with the viral fusion factor (F glycoprotein). Active virions competed effectively with the binding of 125I-labeled Sendai virions (or purified HN glycoprotein), whereas no competition was observed with inactivated Sendai virus. The results of the present work clearly show that protein blotting can be used to identify virus receptors in cell membrane preparations.
D000818:Animals; D004229:Dithiothreitol / Q000494:pharmacology; D004910:Erythrocyte Membrane / Q000032:analysis; D006017:Glycolipids / Q000378:metabolism; D006021:Glycophorins / Q000378:metabolism; D015707:HN Protein; D006801:Humans; D007457:Iodine Radioisotopes; D008024:Ligands; D010222:Parainfluenza Virus 1, Human / Q000378:metabolism*; D011817:Rabbits; D011991:Receptors, Virus / Q000032:analysis*; D014759:Viral Envelope Proteins / Q000378:metabolism
D006017:Glycolipids; D006021:Glycophorins; D015707:HN Protein; D007457:Iodine Radioisotopes; D008024:Ligands; D011991:Receptors, Virus; D014759:Viral Envelope Proteins; D004229:Dithiothreitol