The binding of the chromosomal protein HMG-2a to DNA regions of reduced stabilities.
The interaction of immunopurified high mobility group 2a protein (HMG-2a) with DNA was examined by the nitrocellulose filter binding assay. The relative binding activity of HMG-2a for synthetic polynucleotides was: (dI).(dC) greater than (dA-dT).(dA-dT) greater than (dA).(dT) much greater than (dG).(dC) greater than (dG-dC).(dG-dC). The protein also exhibited a marked preference for (A + T)-rich restriction fragments derived from rat and Drosophila satellites, yeast centromeres, phage lambda, and the ovalbumin gene and its 5' flanking sequences. These preferential DNA interactions occurred at ionic strengths and temperatures within the physiological range which argue for an in vivo role of DNA stability in dictating the genomic distribution of the large Mr HMG proteins.
D000818:Animals; D010582:Bacteriophage lambda / Q000235:genetics; D002417:Cattle; D002645:Chickens; D002843:Chromatin / Q000032:analysis; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004279:DNA, Viral / Q000378:metabolism*; D015246:Deoxyribonuclease EcoRI; D015252:Deoxyribonucleases, Type II Site-Specific; D006609:High Mobility Group Proteins / Q000378:metabolism*; D011067:Poly dA-dT / Q000378:metabolism; D051381:Rats
D002843:Chromatin; D004279:DNA, Viral; D006609:High Mobility Group Proteins; D011067:Poly dA-dT; D004262:DNA Restriction Enzymes; D015246:Deoxyribonuclease EcoRI; D015252:Deoxyribonucleases, Type II Site-Specific; C032229:GANTC-specific type II deoxyribonucleases; C024405:GATC-specific type II deoxyribonucleases; C024403:GGCC-specific type II deoxyribonucleases