title: Relationship between antibody titres against human cytomegalovirus detected by enzyme immunoassay and titres to individual viral polypeptides studied by immunoblotting.
abstract: Several sera with different IgG and IgM titres measured by enzyme immunoassay were studied in serial dilutions for their reactivity with human Cytomegalovirus structural polypeptides separated in SDS-PAGE and electrotransferred to nitrocellulose. The different immunogenicity of the viral proteins is documented and a hypothetical evolution of the humoral immune response to individual polypeptides is presented.
mesh_terms: D000914:Antibodies, Viral / Q000032:analysis*; D003587:Cytomegalovirus / Q000276:immunology*; D003586:Cytomegalovirus Infections / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D007158:Immunologic Techniques; D007700:Kinetics; D010455:Peptides / Q000276:immunology; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000276:immunology; D014764:Viral Proteins / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D010455:Peptides; D014764:Viral Proteins