Peptide mapping of the group-specific antigens from the Australian bluetongue virus (BTV-20) and serotypes from southern Africa and North America.
The Australian bluetongue virus (BTV-20) was compared with six serotypes isolated in southern Africa and North America by peptide mapping of the virus proteins with group antigen properties. The p7 group antigens from each of the seven serotypes analysed did not have identical primary structures and a comparison of shared and unique tryptic peptides has been used as a means of estimating virus relationships. Whereas serological studies have suggested that BTV-20 is closely related to BTV serotypes 4 and 17, comparative peptide mapping of p7 indicates a different set of relationships with viruses from both southern Africa and North America. In contrast with cross-immune precipitation results, peptide mapping of p3 suggest that this protein is not a group specific antigen.
D000956:Antigens, Viral / Q000032:analysis*; D001315:Australia; D001820:Bluetongue virus / Q000145:classification* / Q000276:immunology; D009656:North America; D010446:Peptide Fragments / Q000032:analysis; D012087:Reoviridae / Q000145:classification*; D012703:Serotyping; D013019:South Africa; D014764:Viral Proteins / Q000032:analysis*
D000956:Antigens, Viral; D010446:Peptide Fragments; D014764:Viral Proteins