HLA-DR genotyping by restriction fragment length polymorphism analyses.
We have established unique restriction fragment length polymorphism (RFLP) patterns characteristic of homozygous typing cells (HTCs) for HLA-DR-1 through HLA-DR-8 haplotypes. These RFLP patterns were found to segregate in family members and correlate 100% with HLA-DR antibody phenotyping. The RFLP patterns were used to type chronic myelocytic leukemic cells which have a Philadelphia translocation from 23 randomly selected Caucasoid patients. The results show an alternative method for the determination of the HLA-DR types without using live cells and to study disease association with the HLA-DR region.
D002460:Cell Line; D004262:DNA Restriction Enzymes; D005838:Genotype; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II / Q000235:genetics*; D006801:Humans; D009693:Nucleic Acid Hybridization; D010375:Pedigree; D010677:Philadelphia Chromosome; D011110:Polymorphism, Genetic
D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II; D004262:DNA Restriction Enzymes