Radiation protection of E. coli strains by cysteamine in the presence of oxygen.
The survival of various E. coli K12 strains with defects in the rec system have been measured after gamma-irradiation in air in the presence (0.1 mol dm-3) or in the absence of cysteamine. The results confirm those of Bresler et al. (1978) indicating that the protection by cysteamine in the presence of oxygen is due to an influence on enzymatic repair. The low protection by cysteamine of wild-type cells pretreated with chloramphenicol which prevents protein synthesis, supports the above conclusion. The reason for the absence of a protective effect by OH radical scavenging and H-atom donation is discussed. It is proposed that DNA peroxyl radicals are formed during irradiation in the presence of oxygen and that they are transformed into hydroperoxides by H-atom donation from the intracellular glutathione and the added cysteamine. These hydroperoxides are still dangerous for the cell as indicated by the protective action of glutathione peroxidase observed by Marklund et al. (1984).
D003543:Cysteamine / Q000494:pharmacology*; D004247:DNA / Q000528:radiation effects; D004249:DNA Damage; D004260:DNA Repair; D004926:Escherichia coli / Q000528:radiation effects*; D006878:Hydroxides; D017665:Hydroxyl Radical; D010100:Oxygen / Q000494:pharmacology*
D006878:Hydroxides; D017665:Hydroxyl Radical; D003543:Cysteamine; D004247:DNA; D010100:Oxygen