title: Sequence analysis of a KpnI family member near the 3' end of human beta-globin gene.
abstract: We determined the complete nucleotide sequence (6125 bp) of a full-length member of human KpnI family, designated T beta G41, which is located about 3 kb downstream from the beta-globin gene. Comparison of the sequence with the KpnI family sequence compiled by Singer revealed that a new 131 bp sequence is present in the T beta G41. Hybridization analyses showed that a few thousand of human KpnI family members are carrying this additional sequence. Computer search of DNA databases for T beta G41-homologous sequence showed that some T beta G41-homologous sequences were closely associated with pseudogenes. The T beta G41 sequence also showed significant sequence homology with ChBlym-1, a transferrin-like transforming gene of chicken. Furthermore, an amino acid sequence deduced from the T beta G41 nucleotide sequence revealed a relatively-high homology to those of human transferrin and lactotransferrin.
mesh_terms: D001482:Base Composition; D001483:Base Sequence; D003001:Cloning, Molecular; D003201:Computers; D004262:DNA Restriction Enzymes / Q000235:genetics / Q000302:isolation & purification*; D015252:Deoxyribonucleases, Type II Site-Specific; D005796:Genes; D005914:Globins / Q000235:genetics*; D006801:Humans; D010957:Plasmids; D012689:Sequence Homology, Nucleic Acid
chemical_list: D005914:Globins; D004262:DNA Restriction Enzymes; D015252:Deoxyribonucleases, Type II Site-Specific; C029312:GGTACC-specific type II deoxyribonucleases