title: Babesia bovis: purification and concentration of merozoites and infected bovine erythrocytes.
abstract: Babesia bovis merozoites, externalized by removal of infected erythrocytes from ordinary culture conditions, were completely separated from red blood cells and stroma by centrifugation in a Percoll gradient. A merozoite band formed at a point corresponding to about 1.087 g/ml specific density. Infected red blood cells were concentrated approximately fourfold to obtain greater than 49.0% parasitemia after centrifugation in Percoll. Most highly enriched fractions positioned between 1.121 and 1.123 g/ml specific density. Full parasite viability was retained.
mesh_terms: D000818:Animals; D001403:Babesia / Q000166:cytology / Q000302:isolation & purification*; D001404:Babesiosis / Q000097:blood*; D002417:Cattle; D002499:Centrifugation, Density Gradient / Q000379:methods; D004912:Erythrocytes / Q000469:parasitology*; D011205:Povidone; D012822:Silicon Dioxide
chemical_list: C016039:Percoll; D012822:Silicon Dioxide; D011205:Povidone