title: Predicted sequence of the host-protective immunogen of infectious bursal disease virus.
abstract: The genome of Australian strain 002-73 of infectious bursal disease virus (IBDV) has been cloned as cDNA fragments into an expression library based on pUR plasmid vectors. Recombinant colonies were selected with a monoclonal antibody specific for the 32-kDa host-protective immunogen of IBDV and fully characterized by nucleotide sequence analysis. The amino acid sequence of several tryptic peptides derived from the native 32-kDa structural protein has confirmed the coding region assignment and nucleotide sequence data. We believe this to be the first published sequence of a birnavirus-encoded protein and these data may provide the basis for an effective subunit vaccine against IBDV.
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D004247:DNA / Q000235:genetics; D004274:DNA, Recombinant; D005814:Genes, Viral; D007243:Infectious bursal disease virus / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D012087:Reoviridae / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology*; D014765:Viral Vaccines
chemical_list: D004274:DNA, Recombinant; D012367:RNA, Viral; D014764:Viral Proteins; D014765:Viral Vaccines; D004247:DNA