Putative second messengers affect cell coupling in the seminiferous tubules.
Junctional transfer of ions in monolayer primary cultures of Sertoli cells and in intact seminiferous tubules from 20 day-old rats has been investigated by using electrophysiological techniques. The electrotonic coupling of both intact tubule and monolayer culture was inhibited by the presence of dibutyryl cyclic AMP (10(-4) M) in the medium. In contrast, 1-oleoyl-2-acetylglycerol (10(-5) M) and 12-O-tetradecanoyl-phorbol-13-acetate (10(-7) M) decreased the junctional resistivity and increased the extent of the coupling in immature tubules. The significance of this modulation of the cell coupling in the seminiferous epithelium is discussed.
D000818:Animals; D003994:Bucladesine / Q000494:pharmacology*; D002450:Cell Communication / Q000187:drug effects*; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000502:physiology*; D004075:Diglycerides / Q000494:pharmacology; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012671:Seminiferous Tubules / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D012708:Sertoli Cells / Q000166:cytology / Q000502:physiology; D013737:Testis / Q000166:cytology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D004075:Diglycerides; D003994:Bucladesine; C038237:1-oleoyl-2-acetylglycerol; D000242:Cyclic AMP; D013755:Tetradecanoylphorbol Acetate