Low degree of ubiquitination of histone 2A in the dipteran Chironomus tentans.
A polyclonal antibody to ubiquitin has been prepared and shown to react with both ubiquitin and ubiquitinated histone 2A (uH2A). Applying this antibody in Western blotting experiments, we have observed that the salivary glands of Chironomus tentans contain an unusually low amount of uH2A (1% of histone 2A), while the amount of free ubiquitin is as abundant as in other animal cells, e.g. HeLa cells. The same low content of uH2A was also found in diploid epidermal cells of Chironomus origin suggesting that the low amount is not a characteristic of the polytene state of chromatin in salivary gland cells but rather a property of C. tentans as a species. The significance of the low degree of ubiquitination is discussed in relation to the information available on the organization of Chironomus chromatin into unusually large chromomeric entities.
D000818:Animals; D000906:Antibodies; D002683:Chironomidae / Q000378:metabolism*; D002843:Chromatin / Q000032:analysis; D004175:Diptera / Q000378:metabolism*; D006657:Histones / Q000378:metabolism*; D012469:Salivary Glands / Q000032:analysis; D014452:Ubiquitins / Q000378:metabolism*
D000906:Antibodies; D002843:Chromatin; D006657:Histones; D014452:Ubiquitins