The effect of oxidized isoprenaline on the chick embryonic heart.
Intra-amnial administration of isoprenaline (IPRO) to chick embryos induces a number of myocardial lesions. The purpose of the present study was to investigate whether similar changes may also be induced after injection of spontaneously oxidized isoprenaline and commercially obtained adrenochrome. Cardiotoxicity of these substances has been demonstrated in adult animals. IPRO, oxidized IPRO, or adrenochrome were administered intra-amnially to 10-day-old chick embryos at doses of 0.1, 1.0, 10.0, and 100.0 mg X kg-1. Parallel experimental groups received propranolol at a dose of 1 mg X kg-1, 15 s before injection of IPRO or oxidized IPRO. The cAMP level in the heart was determined by radioimmunoassay 2 and 30 min after administration of IPRO, oxidized IPRO, or adrenochrome at a single dose of 10.0 mg X kg-1. It has been found that in embryos the effect of IPRO and oxidized IPRO is dose dependent. The rise in mortality and development of cardiomegaly together with increased hydration and disturbances of the development of coronary vascularization were highly significant starting from the dose of 10 mg X kg-1. Furthermore, both drugs significantly increased cAMP levels in the embryonic heart. On the other hand, the administration of adrenochrome was without any effect. The changes induced by IPRO were prevented by the administration of the beta-blocking agent propranolol; the lesions induced by spontaneously oxidized IPRO were, however, prevented only partially.
D000323:Adrenochrome / Q000633:toxicity*; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D009202:Cardiomyopathies / Q000139:chemically induced*; D002642:Chick Embryo; D000242:Cyclic AMP / Q000032:analysis; D007545:Isoproterenol / Q000031:analogs & derivatives / Q000037:antagonists & inhibitors / Q000633:toxicity*; D009206:Myocardium / Q000032:analysis; D009929:Organ Size / Q000187:drug effects; D010084:Oxidation-Reduction; D011433:Propranolol / Q000494:pharmacology
D000323:Adrenochrome; D011433:Propranolol; D000242:Cyclic AMP; D007545:Isoproterenol