title: Immunocytochemical identification and localization of the different cell types in the pituitary of the seabass (Dicentrarchus labrax).
abstract: Antisera raised against chum salmon prolactin (PRL), trout growth hormone (GH), mammalian adrenocorticotropic hormone (ACTH), thyroid-stimulating hormone (TSH), luteinizing hormone (LH), and alpha-melanophore-stimulating hormone (alpha-MSH) were used to localize PRL, GH, ACTH, gonadotropic, TSH, and MSH cells in the hypophysis of the teleost Dicentrarchus labrax using the unlabeled peroxidase anti-peroxidase method. In the rostral pars distalis, ACTH cells stained very intensively with anti-ACTH; so did the MSH cells in the pars intermedia. The prolactin cells stained very specifically with anti-prolactin without staining the growth hormone cells. In the proximal pars distalis anti-GH, anti-TSH beta, and anti-LH stained selectively the corresponding cells; with these antisera no cross-reaction with any other cell type was observed. Anti-alpha-MSH only stained cells in the pars intermedia. Some cells in the pars intermedia did not react at all; these could correspond to the PAS-positive cells. A characteristic feature was positive staining with anti-LH in some cell groups encircling the pars intermedia, indicating the fact that in the seabass some cells of the proximal pars distalis surround the pars intermedia.
mesh_terms: D000324:Adrenocorticotropic Hormone / Q000032:analysis; D000818:Animals; D005399:Fishes / Q000033:anatomy & histology* / Q000378:metabolism; D013006:Growth Hormone / Q000032:analysis; D006651:Histocytochemistry; D007124:Immunoenzyme Techniques; D007986:Luteinizing Hormone / Q000032:analysis; D009074:Melanocyte-Stimulating Hormones / Q000032:analysis; D010503:Periodic Acid-Schiff Reaction; D010902:Pituitary Gland / Q000032:analysis / Q000166:cytology*; D010908:Pituitary Hormones, Anterior / Q000032:analysis*; D011388:Prolactin / Q000032:analysis; D013972:Thyrotropin / Q000032:analysis
chemical_list: D010908:Pituitary Hormones, Anterior; D000324:Adrenocorticotropic Hormone; D011388:Prolactin; D007986:Luteinizing Hormone; D013972:Thyrotropin; D013006:Growth Hormone; D009074:Melanocyte-Stimulating Hormones