[Human monoclonal antibodies against surface antigens of Langerhans cells from lymphocytes of diabetics transformed by Epstein-Barr virus].
Human monoclonal antibody against islet cell surface antigens was generated from a pre-diabetic patient's peripheral blood lymphocytes transformed with Epstein-Barr virus. Reactivity of these transformed lymphocytes was evaluated using indirect immunofluorescence on rat islet cell suspensions and frozen sections of human pancreas. Several lymphoblastoid cell lines that react with islet cell surface were obtained. Preliminary immunoblots with enriched rat islet cell membrane antigens suggest a reactivity toward a 64 kdalton antigen.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology*; D002472:Cell Transformation, Viral; D002648:Child; D003922:Diabetes Mellitus, Type 1 / Q000097:blood / Q000276:immunology; D005455:Fluorescent Antibody Technique; D004854:Herpesvirus 4, Human; D006801:Humans; D007515:Islets of Langerhans / Q000166:cytology / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology* / Q000382:microbiology; D051381:Rats
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface
Anticorps monoclonaux humains anti-surface des cellules de Langerhans à partir de lymphocytes de diabètiques transformés par le virus Epstein-Barr.