Neuropharmacology of supraoptic nucleus neurons: norepinephrine and gamma-aminobutyric acid receptors.
The neurosecretory neurons in the mammalian hypothalamic supraoptic nucleus receive prominent GABAergic and noradrenergic projections arising from local interneurons and the A1 cells in the ventrolateral medulla, respectively. Intracellular recordings in in vitro perfused hypothalamic explants reveal an abundance of spontaneous inhibitory postsynaptic potentials (IPSPs) and a compound IPSP after electrical stimulation in the diagonal band of Broca area. The sensitivity of both spontaneous and evoked IPSPs to intracellular chloride injection, bicuculline, and pentobarbital is consistent with a GABA-activated, chloride-mediated inhibitory synaptic input. Parallel changes in membrane voltage and conductance are present during applications of GABA and muscimol, with similar sensitivity to ionic manipulation, bicuculline, and pentobarbital. These observations contrast with the consistently excitatory effects that follow either the stimulation of A1 cells or the application of norepinephrine and alpha 1-adrenergic agonists. Norepinephrine induces membrane depolarizations and bursting activity patterns that are blocked by the selective alpha 1 antagonist prazosin. Membrane response to norepinephrine is voltage dependent and is associated with little change in conductance. GABA and norepinephrine are proposed as transmitters in the final central pathways that mediate information to supraoptic vasopressinergic neurons from peripheral baroreceptors and chemoreceptors, respectively.
D000818:Animals; D066298:In Vitro Techniques; D009474:Neurons / Q000187:drug effects* / Q000502:physiology; D009490:Neurosecretory Systems / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D009638:Norepinephrine / Q000494:pharmacology*; D011941:Receptors, Adrenergic / Q000502:physiology; D011963:Receptors, GABA-A / Q000502:physiology*; D013495:Supraoptic Nucleus / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D014667:Vasopressins / Q000378:metabolism; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology
D011941:Receptors, Adrenergic; D011963:Receptors, GABA-A; D014667:Vasopressins; D005680:gamma-Aminobutyric Acid; D009638:Norepinephrine