Biochemical and immunocytochemical evidence for a deficiency of normal interfascicular oligodendroglia in the CNS of the dysmyelinating mutant (md) rat.
Carbonic anhydrase was assayed and carbonic anhydrase and 5'-nucleotidase were localized in the CNS of myelin-deficient mutant rats and normal littermates. The carbonic anhydrase specific activities were reduced by 61% and 29% in the mutants' forebrains and cerebella, respectively, and the total carbonic anhydrase activity in the spinal cords was reduced by 35%. Immunostained cells were found in gray matter from both normal and mutant rats, but, in the mutants, there was a marked deficiency of interfascicular oligodendrocytes in the regions that are normally occupied by white matter. It is suggested that a developmental study could indicate the step(s) at which normal differentiation of interfascicular oligodendroglia is blocked in this mutant.
D015720:5'-Nucleotidase; D000818:Animals; D001921:Brain / Q000201:enzymology / Q000473:pathology*; D002256:Carbonic Anhydrases / Q000172:deficiency; D003711:Demyelinating Diseases / Q000201:enzymology / Q000473:pathology*; D005260:Female; D006651:Histocytochemistry; D007124:Immunoenzyme Techniques; D008297:Male; D009457:Neuroglia / Q000473:pathology*; D009708:Nucleotidases / Q000032:analysis; D009836:Oligodendroglia / Q000473:pathology*; D051381:Rats; D011922:Rats, Mutant Strains; D013116:Spinal Cord / Q000201:enzymology / Q000473:pathology*
D009708:Nucleotidases; D015720:5'-Nucleotidase; D002256:Carbonic Anhydrases