Evaluation of microsomes isolated from fresh and frozen myotomal tissue of Atlantic cod (Gadus morhua).
Microsomes were isolated from fresh and frozen myotomal tissue of Atlantic cod by two procedures. Electron microscopy revealed one method to yield microsomes containing greater quantities of myofibrillar debris than the other and this was reflected as reduced 5'-nucleotidase, acid phosphatase and succinate dehydrogenase marker enzyme activity. Overnight freezing of myotomal tissue did not affect the marker enzyme activity of microsomes isolated by either procedure. Morphological changes were observed among microsomes prepared from myotomal tissue retained for 8 weeks at -30 degrees C. Accordingly, 5'-nucleotidase was marginally elevated and acid phosphatase and succinate dehydrogenase activity reduced in comparison to fresh microsome preparations.
D015720:5'-Nucleotidase; D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D002458:Cell Fractionation; D002784:Cholesterol / Q000032:analysis; D005399:Fishes; D005615:Freezing; D008854:Microscopy, Electron; D008861:Microsomes / Q000201:enzymology / Q000648:ultrastructure*; D009132:Muscles / Q000201:enzymology / Q000648:ultrastructure*; D009708:Nucleotidases / Q000378:metabolism; D010743:Phospholipids / Q000032:analysis; D013385:Succinate Dehydrogenase / Q000378:metabolism; D014021:Tissue Preservation
D010743:Phospholipids; D002784:Cholesterol; D013385:Succinate Dehydrogenase; D009708:Nucleotidases; D000135:Acid Phosphatase; D015720:5'-Nucleotidase