title: Enhanced formation of ethane and n-pentane by rat hepatocytes in the presence of dimethyl sulfoxide.
abstract: Incubation of rat hepatocytes with 14 mM dimethyl sulfoxide (DMSO) produced an increase in the formation of ethane, measured by capillary column gas chromatography, to 18.0 pmoles/hr/10(7) cells from 11.2 pmoles/hr/1-(7) cells from 5.6 pmoles/hr/10(7) cells in control hepatocytes. This was about one-third the stimulation of ethane and n-pentane formation produced by incubation of hepatocytes with 13 mM carbon tetrachloride. DMSO-stimulated ethane and n-pentane formation was inhibited up to 63% by 0.1 microM alpha-tocopherol and up to 89% by N2. Formation of dimethylsulfide from DMSO by hepatocytes was the same in air and N2. DMSO increased methane production by hepatocytes to 31.3 pmoles/hr/10(7) cells from 6.9 pmoles/hr/10(7) cells in control hepatocytes. Although DMSO apparently stimulated lipid peroxidation by hepatocytes, as measured by ethane and n-pentane formation, there was no increase in the formation of thiobarbituric acid reactive material. DMSO was not toxic to hepatocytes, measured by release of cytosolic lactate dehydrogenase, over a 2-hr incubation. Possible mechanisms for the increase in alkane formation by DMSO are discussed.
mesh_terms: D000818:Animals; D002251:Carbon Tetrachloride / Q000494:pharmacology; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D006878:Hydroxides; D017665:Hydroxyl Radical; D066298:In Vitro Techniques; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008054:Lipid Peroxides / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008697:Methane / Q000378:metabolism*; D010100:Oxygen / Q000032:analysis; D010420:Pentanes / Q000378:metabolism*; D051381:Rats
chemical_list: D006878:Hydroxides; D008054:Lipid Peroxides; D010420:Pentanes; D017665:Hydroxyl Radical; C033353:pentane; D002251:Carbon Tetrachloride; D007770:L-Lactate Dehydrogenase; D008697:Methane; D010100:Oxygen; D004121:Dimethyl Sulfoxide