title: The nucleotide sequence of the yeast MEL1 gene.
abstract: The complete nucleotide sequence of the MEL1 gene of the yeast, Saccharomyces cerevisiae, encoding alpha-galactosidase was determined. The nucleotide sequence contains an open reading frame of 1413 bp encoding a protein of 471 amino acids. Comparison with the known N-terminal amino acid sequence of the mature secreted protein indicated that alpha-galactosidase is synthesized as a precursor with an N-terminal signal sequence of 18 amino acids. The general features of this signal peptide resemble those of other yeast signal peptides. Molecular weight of the mature alpha-galactosidase polypeptide deduced from the nucleotide sequence is 50.049 kd. The 5' regulatory region has sequences in common with other yeast genes regulated by the GAL4-protein.
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D003062:Codon; D004271:DNA, Fungal / Q000235:genetics; D004720:Endonucleases; D005696:Galactosidases / Q000235:genetics*; D005786:Gene Expression Regulation; D005796:Genes; D005800:Genes, Fungal; D008970:Molecular Weight; D011487:Protein Conformation; D021382:Protein Sorting Signals; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D015719:Single-Strand Specific DNA and RNA Endonucleases; D012995:Solubility; D014157:Transcription Factors / Q000235:genetics; D000519:alpha-Galactosidase / Q000235:genetics*
chemical_list: D003062:Codon; D004271:DNA, Fungal; D021382:Protein Sorting Signals; D014157:Transcription Factors; D004720:Endonucleases; D015719:Single-Strand Specific DNA and RNA Endonucleases; D005696:Galactosidases; D000519:alpha-Galactosidase