Cell surface receptors for sulphated polysaccharides: a potential marker for macrophage subsets.
The expression of a diverse array of receptors for sulphated polysaccharides on lymphocytes has been demonstrated by Parish & Snowden (1985). This paper presents evidence to suggest that other cell types, namely macrophages, polymorphonuclear leucocytes, mast cells and fibroblasts, can bind similar polysaccharides. Using a rosetting assay and eleven structurally unique polysaccharides, each cell type was observed to bind a characteristic array of these polysaccharides. Analysis of the polysaccharide reactivity of macrophages revealed that BCG-activated and thioglycollate-elicited macrophages express an expanded repertoire of reactivity compared to resident peritoneal macrophages. For example, only thioglycollate-elicited macrophages, but not resident and BCG-activated peritoneal macrophages, reacted with the glycosaminoglycans, chondroitin-4-sulphate, chondroitin-6-sulphate and dermatan sulphate, while both BCG- and thioglycolate-activated, but not resident peritoneal macrophages, bound pentosan polysulphate-coupled sheep erythrocytes. The expression of the receptors for chondroitin-4 and -6-sulphate was observed to be cyclic and peaked at 2 and 5-6 days after thioglycollate treatment. Preliminary analyses of the functional significance of the observed binding of polysaccharides to macrophages revealed that heparin, fucoidan and kappa-carrageenen were specifically endocytosed. However, endocytosis of all other test polysaccharides was not observed. Finally, polysaccharide-coupled sheep erythrocytes were not phagocytosed, even though they interacted strongly with the macrophage surface. The possible relevance of these observations to an inflammatory response and as a means of identifying cellular subsets is discussed.
D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D004705:Endocytosis; D008264:Macrophages / Q000145:classification* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009163:Mycobacterium bovis; D009504:Neutrophils / Q000276:immunology; D010587:Phagocytosis; D011134:Polysaccharides / Q000378:metabolism; D011946:Receptors, Antigen / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000378:metabolism; D012397:Rosette Formation; D013864:Thioglycolates / Q000494:pharmacology
D011134:Polysaccharides; D011946:Receptors, Antigen; D011956:Receptors, Cell Surface; D013864:Thioglycolates