OH-induced free radicals in uridine studied by a method combining ESR, spin-trapping, and liquid chromatography.
Free radicals produced by the reactions of OH radicals with uridine were investigated by a method combining ESR, spin-trapping, and liquid chromatography. A N2O-saturated aqueous solution of uridine, containing 2-methyl-2-nitrosopropane as a spin-trap, was X-irradiated and the resulting spin-adducts were separated by gel permeation chromatography and reverse-phase HPLC. ESR and uv-absorbance spectra obtained from the separated spin-adducts show that 5-yl and 6-yl radicals are produced by OH addition to the 5,6 double bond of the base moiety. It is also shown that radicals due to H abstraction from the sugar moiety at the C-4' and C-5' positions are produced.
D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004578:Electron Spin Resonance Spectroscopy; D005609:Free Radicals; D006878:Hydroxides; D009603:Nitroso Compounds; D013113:Spin Labels; D014529:Uridine / Q000528:radiation effects*
D005609:Free Radicals; D006878:Hydroxides; D009603:Nitroso Compounds; D013113:Spin Labels; C031356:hydroxide ion; C034526:tert-nitrosobutane; D014529:Uridine