Indirect technique for the estimation of cAMP-dependent and Ca2+/calmodulin-dependent phospholamban phosphorylation state in canine heart in vivo.
An indirect technique was employed to estimate the in vivo phosphorylation state of phospholamban in preparations from dog hearts depleted from catecholamines and from dog hearts treated with isoproterenol. This method allows the separate detection of cAMP-dependent and Ca2+/calmodulin-dependent phospholamban phosphorylation. The data obtained demonstrate the phosphorylation of both the cAMP-dependent and the Ca2+/calmodulin-dependent phosphorylatable site of phospholamban in response to the beta-adrenergic agonist isoproterenol in canine heart in vivo.
D000818:Animals; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D002147:Calmodulin / Q000378:metabolism; D000242:Cyclic AMP / Q000378:metabolism; D004285:Dogs; D066298:In Vitro Techniques; D007545:Isoproterenol / Q000494:pharmacology; D009206:Myocardium / Q000378:metabolism*; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism*; D012110:Reserpine / Q000494:pharmacology; D000099211:Phospholamban
D002135:Calcium-Binding Proteins; D002147:Calmodulin; D000242:Cyclic AMP; D007545:Isoproterenol; D011494:Protein Kinases; D012110:Reserpine; D000099211:Phospholamban