Human bone cell cultures: a new model for studying the mechanism of action of calcitonin.
An investigation on cell cultures obtained from temporal human bone fragments showed that they provide a suitable model for studying the mechanism involved in calcitonin action on bone cells. Furthermore they demonstrated: a transitory increase in 45Ca uptake that returned to control values ten minutes after the hormone was added; a relation between 45Ca uptake and increased cAMP concentrations when these were measured at the same time intervals; a reproduction of the salmon calcitonin (sCT) effect after incubation of the cultures with either db-cAMP or db-cGMP and inhibition of 45Ca uptake and parallel decrease in cAMP levels with propanol. These results suggest that in human bone cell cultures, sCT acts as a temporary promoter of 45Ca uptake, probably by activating an adenylate-cyclase system through a beta-receptor.
D001842:Bone and Bones / Q000166:cytology / Q000187:drug effects*; D002116:Calcitonin / Q000494:pharmacology*; D002132:Calcium Radioisotopes / Q000378:metabolism; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000032:analysis; D006152:Cyclic GMP / Q000032:analysis; D006801:Humans; D011433:Propranolol / Q000494:pharmacology; D013701:Temporal Bone / Q000166:cytology; D013997:Time Factors
D002132:Calcium Radioisotopes; D002116:Calcitonin; D011433:Propranolol; D000242:Cyclic AMP; D006152:Cyclic GMP