[Detection of superoxide radicals in intact heart mitochondria by spin trapping].
Tiron (4,5-dihydroxybenzene-1,3-disulfonic acid, disodium salt) has been used as a spin trap to detect superoxide radicals produced by the rat heart mitochondria. In the presence of the oxidative phosphorylation uncoupler carbonyl cyanide (3-chlorophenyl)-hydrazone the superoxide production rate increases.
D000818:Animals; D004578:Electron Spin Resonance Spectroscopy; D005609:Free Radicals; D066298:In Vitro Techniques; D008929:Mitochondria, Heart / Q000378:metabolism*; D051381:Rats; D013113:Spin Labels; D013481:Superoxides / Q000378:metabolism*
D005609:Free Radicals; D013113:Spin Labels; D013481:Superoxides
Registratsiia obrazovaniia superoksidnykh radikalov intaktnymi mitokhondriiami serdtsa s pomoshch'iu spinovoĭ lovushki.