Detection of low numbers of poliovirus 1 in oysters: collaborative study.
A collaborative study was performed to evaluate a method for determining numbers of poliovirus 1 in oysters. Commonly available laboratory equipment and materials were used. Raw oysters in the shell were shipped to each investigator along with 12 tubes of unknown concentrations of virus. Six 100 g duplicate oyster samples were analyzed by 5 collaborating laboratories. Each analyst used a prescribed procedure for diluting the inocula. Two samples contained approximately 100 plaque-forming units (pfu)/100 g, 2 samples contained approximately 50 pfu/100 g, and the other 2 contained approximately 25 pfu/100 g. Recoveries varied from 35 to 55% at inocula levels of 30-100 pfu/100 g. The limit of detectability was hypothesized to be 14 pfu in a 100 g sample when the recovery was 35%. The method has been adopted official first action.
D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D005516:Food Microbiology; D010125:Ostreidae / Q000382:microbiology*; D017955:Poliovirus / Q000302:isolation & purification*; D010948:Viral Plaque Assay