Calcitonin gene-related peptide stimulates cyclic AMP formation in rat aortic smooth muscle cells.
In rat aortic smooth muscle cells in culture, calcitonin gene-related peptide stimulated cAMP formation in a dose-dependent manner, half-maximally effective at 0.5 to 1 nM. There was no effect on formation of cGMP, which was increased 300-fold in the same experiments by atriopeptin or sodium nitroprusside. The vasodilator effect of CGRP in rat aorta requires an intact endothelium, indicating that increase in vascular smooth muscle cAMP is not in itself sufficient to bring about relaxation. cAMP is probably a mediator of CGRP action in vascular smooth muscle.
D000818:Animals; D009320:Atrial Natriuretic Factor / Q000494:pharmacology; D000001:Calcimycin / Q000494:pharmacology; D015740:Calcitonin Gene-Related Peptide; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000096:biosynthesis*; D006152:Cyclic GMP / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005260:Female; D007545:Isoproterenol / Q000494:pharmacology; D009126:Muscle Relaxation / Q000187:drug effects; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000494:pharmacology*; D009599:Nitroprusside / Q000494:pharmacology; D010656:Phenylephrine / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains
D009419:Nerve Tissue Proteins; D009599:Nitroprusside; D010656:Phenylephrine; D000001:Calcimycin; D009320:Atrial Natriuretic Factor; D000242:Cyclic AMP; D006152:Cyclic GMP; D015740:Calcitonin Gene-Related Peptide; D007545:Isoproterenol