Partial immunochemical identity between (Na+ + K+)-ATPase and a membrane component extractable with chloroform: methanol.
An IgG fraction prepared from an antiserum against a holoenzyme preparation of (Na+ + K+)-ATPase precipitated a single antigen when samples of holoenzyme were subjected to crossed immunoelectrophoresis but precipitated an additional, immunochemically-related antigen when a plasma membrane-enriched fraction was subjected to crossed immunoelectrophoresis under the same conditions. The immunochemically-related antigen could be extracted from the plasma membrane fraction with CHCl3:CH3OH.
D000818:Animals; D000941:Antigens / Q000302:isolation & purification; D002462:Cell Membrane / Q000201:enzymology / Q000276:immunology; D002725:Chloroform; D004372:Ducks; D007120:Immunochemistry; D007123:Immunoelectrophoresis, Two-Dimensional; D000432:Methanol; D012491:Salt Gland / Q000201:enzymology / Q000276:immunology; D000254:Sodium-Potassium-Exchanging ATPase / Q000276:immunology* / Q000302:isolation & purification
D000941:Antigens; D002725:Chloroform; D000254:Sodium-Potassium-Exchanging ATPase; D000432:Methanol