Expression of aldolase A messenger RNAs in human adult and foetal tissues and in hepatoma.
3 specific cDNA clones for human aldolase A were isolated from a human muscle library. One of them was subcloned in M 13 phage, then used as a probe to investigate the patterns and the levels of aldolase A mRNA in various human tissues. Two mRNA species differing in length were observed. The lighter one -1550 bases- was found specific to skeletal muscle; its amount increased during muscle development. The heavier aldolase A mRNA -1650 bases- accounted for foetal and ubiquitous presence of aldolase A isozyme. The resurgence of aldolase A in hepatomas occurred through this latter mRNA species.
D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000201:enzymology*; D004247:DNA / Q000032:analysis; D004262:DNA Restriction Enzymes; D005333:Fetus / Q000201:enzymology; D005634:Fructose-Bisphosphate Aldolase / Q000096:biosynthesis / Q000235:genetics*; D006801:Humans; D007527:Isoenzymes / Q000096:biosynthesis / Q000235:genetics; D008113:Liver Neoplasms / Q000201:enzymology*; D009132:Muscles / Q000201:enzymology; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000502:physiology*; D051381:Rats
D007527:Isoenzymes; D012333:RNA, Messenger; D004247:DNA; D004262:DNA Restriction Enzymes; D005634:Fructose-Bisphosphate Aldolase