Direction of travel of RecBC recombinase through bacteriophage lambda DNA.
We examined linkage relationships for RecBC-mediated recombination in lytic cycle crosses of lambda phages bearing two cohesive end sites (cos) oriented in the same direction. The relationships obtained imply that a given recombinant tends to be packaged from the cos site that is the nearer one to the right of the exchange. In view of the previously established coupling of entry of a recombinase at a cos cut and initiation of DNA packaging by that cos cut, these results imply that the recombinase (presumably the RecBC gene product) enters lambda's chromosome at the right end.
D010582:Bacteriophage lambda / Q000187:drug effects / Q000235:genetics*; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics; D029968:Escherichia coli Proteins; D043211:Exodeoxyribonuclease V; D005090:Exodeoxyribonucleases / Q000378:metabolism*; D010641:Phenotype; D013045:Species Specificity; D014161:Transduction, Genetic; D014295:Trimethoprim / Q000494:pharmacology
D004279:DNA, Viral; D029968:Escherichia coli Proteins; D014295:Trimethoprim; D005090:Exodeoxyribonucleases; D043211:Exodeoxyribonuclease V; C468322:exodeoxyribonuclease V, E coli; D004262:DNA Restriction Enzymes