A computer-assisted rapid-scanning spectrophotometer with applications to tissues in vitro and in vivo.
A flexible approach is described to acquire, store, retrieve, and manipulate the large volume of data provided by a rapid-scanning spectrophotometer designed for biological preparations. Data can be displayed in spectral and kinetic formats. This spectrophotometer provides a means to simultaneously measure the concentrations of components of light scattering preparations and to record chemical reactions as they occur. Although application of this instrument for the study of reduction/oxidation shifts of mitochondrial respiratory chain components is emphasized, the design is suitable for many spectrophotometric applications.
D000818:Animals; D003201:Computers; D003574:Cytochrome c Group / Q000378:metabolism; D004579:Electron Transport; D004867:Equipment Design; D006454:Hemoglobins / Q000378:metabolism; D066298:In Vitro Techniques; D008027:Light; D008838:Microcomputers; D008905:Minicomputers; D008928:Mitochondria / Q000378:metabolism; D055095:Optics and Photonics / Q000295:instrumentation; D010084:Oxidation-Reduction; D051381:Rats; D012984:Software; D013053:Spectrophotometry / Q000295:instrumentation*
D003574:Cytochrome c Group; D006454:Hemoglobins