[Genetic analysis of Escherichia coli min81 mutation blocking the development of bacteriophage Mu].
Data characterizing mim81 mutation obtained by the method for direct selection of transposition mutations are presented. The development of Mu is shown to be dramatically suppressed in the mutant strain both upon infection and after induction from the lysogenic state. Frequencies of lysogenization and mini-Mu-dependent formation of cointegrates in the mutant strain are comparable with those in the wild-type strain. Mu development prohibition is removed if expression of early Mu gene is provided from the modified Pe promoter. The results obtained make us believe that the mechanism of mim81 mutation action involves reduction of early gene expression to the level that is sufficient for Mu DNA integration into the chromosome during infection and for single replicative events, but insufficient for vegetative development of bacteriophage Mu.
D010583:Bacteriophage mu / Q000235:genetics*; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics*; D009154:Mutation; D010957:Plasmids; D014162:Transfection
D004251:DNA Transposable Elements
Geneticheskoe izuchenie mutatsii min81 Escherichia coli, blokiruiushcheĭ razvitie bakteriofaga Mu.