Inositol phospholipid and intracellular calcium metabolism in B lymphocytes stimulated with antigen.
Stimulation of B lymphocytes by anti mu antibody can activate the phosphatidylinositol pathway, but B cell activation by LPS does not involve this pathway. This study was done to determine if stimulation of B lymphocytes by their specific antigen involves this important activation pathway. We showed that levels of IP2 and IP3 increase while PIP and PIP2 decline when dinitrophenyl specific B lymphocytes are stimulated with the antigen DNP-Ficoll. Intracellular calcium concentration also increases with this stimulus. Thus, antigen stimulation of B lymphocytes is associated with activation of phosphatidylinositol pathway.
D000941:Antigens / Q000494:pharmacology; D001402:B-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D002118:Calcium / Q000097:blood*; D002460:Cell Line; D005362:Ficoll / Q000031:analogs & derivatives / Q000494:pharmacology; D008213:Lymphocyte Activation; D010716:Phosphatidylinositols / Q000097:blood*
D000941:Antigens; C031007:DNP-Ficoll; D010716:Phosphatidylinositols; D005362:Ficoll; D002118:Calcium