Spot hybridization for the detection of adenoviruses and enteroviruses.
Nucleic acid spot hybridization was used for detection of adenovirus DNA directly in clinical specimens and enterovirus RNA in infected cells. The test gave results comparable to those obtained with antigen detection assays for adenoviruses. Spot hybridization could also be used for detection of enterovirus growth in cell cultures after a single passage from clinical specimens.
D000256:Adenoviridae / Q000032:analysis* / Q000145:classification / Q000235:genetics; D000257:Adenoviridae Infections / Q000175:diagnosis* / Q000382:microbiology; D002478:Cells, Cultured; D002648:Child; D004247:DNA; D004269:DNA, Bacterial / Q000032:analysis*; D004279:DNA, Viral / Q000032:analysis*; D004770:Enterovirus / Q000032:analysis* / Q000145:classification / Q000235:genetics; D004769:Enterovirus Infections / Q000175:diagnosis* / Q000382:microbiology; D005243:Feces / Q000382:microbiology; D005759:Gastroenteritis / Q000382:microbiology; D005819:Genetic Markers; D006801:Humans; D009305:Nasopharynx / Q000382:microbiology; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000032:analysis; D014776:Virus Cultivation
D004269:DNA, Bacterial; D004279:DNA, Viral; D005819:Genetic Markers; D012367:RNA, Viral; D004247:DNA