title: CAT vectors for analysis of eukaryotic promoters and enhancers.
abstract: We have constructed two sets of plasmids for analysis of factors affecting mammalian gene expression. The pOCAT series contains a bacterial chloramphenicol-resistance expression unit (cat) and no eukaryotic promoter. The pUTKAT series contains the same cat unit under the control of the thymidine-kinase promoter of Herpes simplex virus. These plasmids are designed for testing effects of inserted regulatory elements on cat expression after transient transfection of mammalian cells in culture. We demonstrate here that the pOCAT series is useful for studying activities of inserted eukaryotic promoters, and the pUTKAT series is useful for studying activities of inserted eukaryotic enhancers.
mesh_terms: D000123:Acetyltransferases / Q000235:genetics*; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase; D004742:Enhancer Elements, Genetic; D005057:Eukaryotic Cells; D005786:Gene Expression Regulation / Q000187:drug effects; D005809:Genes, Regulator; D005814:Genes, Viral; D005822:Genetic Vectors; D005938:Glucocorticoids / Q000494:pharmacology; D008322:Mammals / Q000235:genetics; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000235:genetics*; D011994:Recombinant Proteins / Q000235:genetics*; D018139:Simplexvirus / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D005938:Glucocorticoids; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D000123:Acetyltransferases; D015500:Chloramphenicol O-Acetyltransferase