Assignment of simian rotavirus SA11 temperature-sensitive mutant groups B and E to genome segments.
Recombinant (reassortant) viruses were selected from crosses between temperature-sensitive (ts) mutants of simian rotavirus SA11 and wild-type human rotavirus Wa. The double-stranded genome RNAs of the reassortants were examined by electrophoresis in Tris-glycine-buffered polyacrylamide gels and by dot hybridization with a cloned DNA probe for genome segment 2. Analysis of replacements of genome segments in the reassortants allowed construction of a map correlating genome segments providing functions interchangeable between SA11 and Wa. The reassortants revealed a functional correspondence in order of increasing electrophoretic mobility of genome segments. Analysis of the parental origin of genome segments in ts+ SA11/Wa reassortants derived from the crosses SA11 tsB(339) X Wa and SA11 tsE(1400) X Wa revealed that the group B lesion of tsB(339) was located on genome segment 3 and the group E lesion of tsE(1400) was on segment 8.
D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular; D003433:Crosses, Genetic; D005814:Genes, Viral; D005838:Genotype; D000882:Haplorhini; D006801:Humans; D009154:Mutation; D010761:Phosphorus Radioisotopes; D012330:RNA, Double-Stranded / Q000096:biosynthesis / Q000302:isolation & purification; D012401:Rotavirus / Q000235:genetics*; D013045:Species Specificity; D013696:Temperature
D010761:Phosphorus Radioisotopes; D012330:RNA, Double-Stranded