Modulation of E-receptor expression on activated T lymphocytes.
Our study indicated that the newly synthesized E-receptor, as measured with 125I-labeled monoclonal anti-E receptor antibody, on activated T lymphocytes were responsible for forming stable E-rosettes at 37 degrees C. Maximum induction of new E-receptor expression required at least 50 hr of culture with polyclonal T-cell activators, phytohemagglutinin, or phorbol myristate acetate. Polyclonal B-cell activator, lipopolysaccharide were not able to induce new E-receptor expression on the surface of T lymphocytes. The expression of the new E-receptor paralleled with the induction of Tac antigen expression. Interleukins 1 and 2 or Interferon-gamma were not able to initiate the induction of new E-receptors. However, a neuropeptide, endorphin exhibited biphasic effect on modulating the E-receptor expression, in the absence of polyclonal activators. As is the case with Tac antigen expression, induction of new E-receptor antigen may be a marker for activated T lymphocytes.
D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D006801:Humans; D007700:Kinetics; D008213:Lymphocyte Activation; D008222:Lymphokines / Q000494:pharmacology; D010835:Phytohemagglutinins / Q000494:pharmacology; D011453:Prostaglandins / Q000502:physiology; D014176:Protein Biosynthesis; D011817:Rabbits; D011863:Radioimmunoassay; D011971:Receptors, Immunologic / Q000032:analysis*; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000276:immunology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7
D000954:Antigens, Surface; D008222:Lymphokines; D010835:Phytohemagglutinins; D011453:Prostaglandins; D011971:Receptors, Immunologic; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7; D013755:Tetradecanoylphorbol Acetate