NAD+-dependent conversion of 20-OH-LTB4 to 20-COOH-LTB4 by a cell-free system of human polymorphonuclear leukocytes.
Human polymorphonuclear leukocytes, but not mononuclear leukocytes, platelets, or erythrocytes, oxidized 20-hydroxy-leukotriene B4 (20-OH-LTB4) to 20-carboxy-LTB4 (20-COOH-LTB4). 20-OH-LTB4 was quantitatively converted to 20-COOH-LTB4 by the sonicate of polymorphonuclear leukocytes in the presence of NAD+, with an optimal pH of about 7.9. NADP+ could not replace NAD+. The conversion was not inhibited by 2 mM pyrazole, a potent inhibitor of alcohol dehydrogenase. When LTB4 was incubated with the sonicate in the presence of NADPH and NAD+, 20-OH-LTB4 and 20-COOH-LTB4 appeared concomitantly with the disappearance of LTB4.
D002474:Cell-Free System; D006801:Humans; D006893:Hydroxyeicosatetraenoic Acids; D066298:In Vitro Techniques; D007975:Leukotriene B4 / Q000031:analogs & derivatives* / Q000378:metabolism*; D009243:NAD / Q000494:pharmacology*; D009504:Neutrophils / Q000378:metabolism*; D010084:Oxidation-Reduction
D006893:Hydroxyeicosatetraenoic Acids; D009243:NAD; D007975:Leukotriene B4; C031571:5,12,20-trihydroxy-6,8,10,14-eicosatetraenoic acid; C031911:20-carboxyleukotriene B4