Viral DNA sequences detected in a hamster liposarcoma induced by bovine papillomavirus type 4.
Following intradermal inoculation of bovine papillomavirus type 4 (BPV-4) into a Syrian hamster, a liposarcoma developed at the inoculation site 20 months later. The DNA of this tumour contained multiple copies of the BPV-4 genome which existed in a free unintegrated state. Unintegrated viral DNA and viral DNA isolated from virus particles from bovine alimentary tract papillomas revealed identical cleavage patterns with CpG methylation-resistant and -sensitive restriction enzymes: apparently there was no gross methylation of CpG sites in either case. The entire BPV-4 genome appeared to be represented in the tumour DNA.
D000818:Animals; D001483:Base Sequence; D052688:Bovine papillomavirus 1 / Q000235:genetics* / Q000472:pathogenicity; D052697:Bovine papillomavirus 4; D002417:Cattle; D006224:Cricetinae; D004273:DNA, Neoplasm / Q000302:isolation & purification; D004279:DNA, Viral / Q000235:genetics* / Q000302:isolation & purification; D008080:Liposarcoma / Q000382:microbiology*; D008647:Mesocricetus; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D027383:Papillomaviridae / Q000235:genetics*
D004273:DNA, Neoplasm; D004279:DNA, Viral