Vasopressin induced production of inositol trisphosphate and calcium efflux in a smooth muscle cell line.
Phosphatidylinositol metabolism and 45Ca2+ efflux were examined in a vascular smooth muscle cell line (A7r5). [Arg 8]Vasopressin stimulated the rapid formation (measurable at 1 sec) of inositol phosphates in a concentration-dependent manner. The time course for formation of inositol phosphates was similar to that for 45Ca2+ efflux from preloaded cells. The efflux of 45Ca2+ in response to [Arg8]vasopressin could be inhibited by a vasopressin antagonist. This supports the hypothesis that inositol 1,4,5-trisphosphate plays a role in vasopressin stimulated calcium mobilisation from an intracellular source in cultured vascular smooth muscle cells.
D000818:Animals; D001127:Arginine Vasopressin / Q000031:analogs & derivatives / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism*; D002132:Calcium Radioisotopes / Q000378:metabolism; D002460:Cell Line; D002712:Chlorides / Q000494:pharmacology; D015544:Inositol 1,4,5-Trisphosphate; D007295:Inositol Phosphates / Q000096:biosynthesis*; D007700:Kinetics; D008094:Lithium / Q000494:pharmacology; D018021:Lithium Chloride; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D013403:Sugar Phosphates / Q000096:biosynthesis*
D002132:Calcium Radioisotopes; D002712:Chlorides; D007295:Inositol Phosphates; D013403:Sugar Phosphates; D001127:Arginine Vasopressin; C028883:argipressin, beta-mercapto beta,beta-cyclopentamethylenepropionic acid(1)-O-methyl-Tyr(2)-Val(4)-; D015544:Inositol 1,4,5-Trisphosphate; D008094:Lithium; D018021:Lithium Chloride; D002118:Calcium