An interferon analogue, [Ala 30,32,33]HuIFN-alpha 2, acting as a HuIFN-alpha 2 antagonist on bovine cells.
We have studied the biological and receptor binding properties of a human alpha 2-interferon (HuIFN-alpha 2) analogue, [Ala30,32,33] HuIFN-alpha 2, which is shown in the accompanying paper (1) to be biologically inactive on homologous cells. Here we demonstrate that this analogue is also devoid of biological activity on bovine MDBK cells. However, whereas the analogue did not inhibit the binding of radiolabeled HuIFN-alpha 2 to WISH cells, it did compete for binding to receptors on the bovine cells. This behavior suggested that [Ala30,32,33] HuIFN-alpha 2 could act as an antagonist of HuIFN-alpha 2 on bovine cells and indeed coaddition of the analogue and native HuIFN-alpha 2 to MDBK cells competitively inhibited both the antiviral and antiproliferative activity of HuIFN-alpha 2.
D000818:Animals; D000998:Antiviral Agents / Q000037:antagonists & inhibitors; D001667:Binding, Competitive; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D006801:Humans; D007370:Interferon Type I; D016898:Interferon-alpha; D007371:Interferon-gamma / Q000037:antagonists & inhibitors* / Q000378:metabolism / Q000494:pharmacology; D011971:Receptors, Immunologic / Q000378:metabolism; D017471:Receptors, Interferon; D011994:Recombinant Proteins; D014721:Vesicular stomatitis Indiana virus / Q000187:drug effects
D000998:Antiviral Agents; D007370:Interferon Type I; D016898:Interferon-alpha; D011971:Receptors, Immunologic; D017471:Receptors, Interferon; D011994:Recombinant Proteins; C047910:interferon alpha 2, Ala(30,32,33)-; D007371:Interferon-gamma