Role of marrow stromal cells in the establishment of a transformed lymphoblastic B-cell line from a normal human subject.
A monoclonal human B-lymphoblastoid cell line (UTMB-460) arose spontaneously from the bone marrow of a normal healthy woman who was seropositive for an EB-virus infection. Chromosomally, the UTMB-460 cells are near tetraploid, with a specific translocation (8;9) (p11.2; p24), and have surface IgMk. The UTMB-460 cells are resistant to killing in vitro by spontaneous and rIFN alpha 2 and rIL-2 stimulated NK cells from the patient and other normal subjects, but are killed by lymphokine activated killer cells. The index patient has not developed leukemia/lymphoma during the follow-up interval of 22 months. The growth of UTMB-460 cells is supported by undefined growth factors in FCS and by BCGF in the absence of FCS. rIL-2 stimulates DNA synthesis by UTMB-460 cells. The UTMB-460 cells were adherent to the normal MSC in the primary culture and show specific heterotypic adherence to normal MSC when compared to skin fibroblasts. In addition, 6/6 normal marrow stromal cells and 4/6 normal skin fibroblasts induced growth of colonies from UTMB-460 cells. These data suggest that MSC interacted with the transformed cells (UTMB-460) in vitro and played a critical role in the establishment of the UTMB-460 cell line.
D000328:Adult; D001402:B-Lymphocytes / Q000276:immunology / Q000473:pathology* / Q000648:ultrastructure; D001853:Bone Marrow / Q000502:physiology*; D002448:Cell Adhesion; D002450:Cell Communication; D002460:Cell Line; D002472:Cell Transformation, Viral; D004247:DNA / Q000096:biosynthesis; D005260:Female; D004854:Herpesvirus 4, Human; D006801:Humans; D007938:Leukemia / Q000473:pathology
D004247:DNA