DNA-mediated transfer and expression of a human DNA repair gene that demethylates O6-methylguanine.
Human liver DNA was transfected into CHO cells (mex-) along with pSV2gpt and colonies were selected first for resistance to mycophenolic acid and then to chloroethylnitrosourea. Transformants were obtained that contained approximately 10,000 molecules of O6-alkylguanine alkyltransferase (mex+) per cell. Their genome contained at least three copies of the human Alu sequence.
D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D004260:DNA Repair; D004262:DNA Restriction Enzymes; D004351:Drug Resistance; D005038:Ethylnitrosourea / Q000031:analogs & derivatives / Q000494:pharmacology; D005260:Female; D005796:Genes; D006147:Guanine / Q000031:analogs & derivatives* / Q000378:metabolism; D006801:Humans; D008099:Liver / Q000378:metabolism; D008780:Methyltransferases / Q000235:genetics*; D009173:Mycophenolic Acid / Q000494:pharmacology; D019853:O(6)-Methylguanine-DNA Methyltransferase; D010053:Ovary; D014170:Transformation, Genetic
C017139:1-(2-chloroethyl)-1-nitrosourea; D006147:Guanine; C008449:O-(6)-methylguanine; D008780:Methyltransferases; D019853:O(6)-Methylguanine-DNA Methyltransferase; D004262:DNA Restriction Enzymes; D009173:Mycophenolic Acid; D005038:Ethylnitrosourea