Radioimmunoassay of adjuvant-associated porcine parvovirus using a monoclonal antibody in a nitrocellulose membrane system.
A quantitative and simple indirect radioimmunoassay (IRIA) was developed for porcine parvovirus (PPV), employing a monoclonal antibody directed against PPV adsorbed to nitrocellulose membrane. The IRIA was equally sensitive to live or inactivated PPV. There was a linear relationship between membrane-bound radioactivity and PPV quantity within a range of 10-80 hemagglutinating (HA) units of virus. Two commercially used adjuvants, aluminum hydroxide (AH) and carboxyvinyl polymer (CP), reduced bound radioactivity in a concentration-dependent manner. At fixed adjuvant concentrations, there were, nevertheless, linear relationships between bound radioactivity and HA units of PPV. Known amounts of PPV were prepared in adjuvants according to commercial vaccine formulations. Using these standards, the PPV content of 16 commercial PPV vaccines was estimated by IRIA. The IRIA may be one practical method of in vitro estimation of antigenic mass in adjuvanted vaccines.
D000276:Adjuvants, Immunologic; D000536:Aluminum Hydroxide; D000818:Animals; D000911:Antibodies, Monoclonal; D000956:Antigens, Viral / Q000032:analysis*; D002264:Carboxylic Acids / Q000276:immunology; D010321:Parvoviridae / Q000276:immunology*; D010322:Parvoviridae Infections / Q000276:immunology; D011145:Polyvinyls / Q000276:immunology; D011863:Radioimmunoassay; D013552:Swine; D014765:Viral Vaccines / Q000276:immunology*
D000276:Adjuvants, Immunologic; D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D002264:Carboxylic Acids; D011145:Polyvinyls; D014765:Viral Vaccines; D000536:Aluminum Hydroxide