title: The interaction of murine cytomegalovirus with murine neutrophils: effect on migratory and phagocytic activities.
abstract: To investigate the interaction of cytomegalovirus (CMV) with neutrophils, we studied the effects of in vitro incubation of murine neutrophils with murine CMV (MCMV). Neutrophils incubated with MCMV for 4 h had depressed chemotactic activity, mean chemotactic index of 1.34 +/- 1.43 for MCMV-treated neutrophils vs 3.22 +/- 2.11 for controls. Engulfment of latex spheres by MCMV-treated neutrophils was also reduced. These differences were not attributable to loss of cell viability. UV-inactivation of MCMV pools abolished the inhibitory effects of MCMV, indicating that these effects were related to infectivity of the virus. Electron microscopic studies at 4 h demonstrated virus particles within the phagosomes of occasional neutrophils. These studies demonstrate that neutrophil functions can be altered by an in vitro interaction with infectious CMV and suggest that a direct effect of CMV on neutrophils could account for the abnormal neutrophil functions observed during animal CMV infections.
mesh_terms: D000327:Adsorption; D000818:Animals; D002634:Chemotaxis, Leukocyte; D003587:Cytomegalovirus / Q000502:physiology* / Q000648:ultrastructure; D051379:Mice; D008815:Mice, Inbred Strains; D008854:Microscopy, Electron; D009504:Neutrophils / Q000382:microbiology / Q000502:physiology* / Q000648:ultrastructure; D010587:Phagocytosis; D014771:Virion / Q000648:ultrastructure