Development of a micro-neutralization test for chikungunya virus.
A rapid, micro-scale focus reduction neutralization test for chikungunya virus was developed. In the test, cell monolayers are prepared in a 96-well tissue culture plate and the PAP (peroxidase-antiperoxidase) staining technique is used for detection of foci of chikungunya virus infected cells. This test is suitable for rapid diagnosis and epidemiological studies of the virus.
D000914:Antibodies, Viral / Q000032:analysis*; D002460:Cell Line; D002646:Chikungunya virus / Q000254:growth & development / Q000276:immunology*; D006801:Humans; D007124:Immunoenzyme Techniques; D009500:Neutralization Tests; D014036:Togaviridae Infections / Q000175:diagnosis*; D010948:Viral Plaque Assay
D000914:Antibodies, Viral