Polyomavirus small T antigen enhances replication of viral genomes in 3T6 mouse fibroblasts.
Transfection of 3T6 cells with a cloned polyomavirus genome encoding only large T antigen resulted in DNA replication with only about 1/10 the efficiency of wild-type viral DNA coding for all three T antigens. This replication defect was at least in part overcome by the simultaneous transfections of polyomavirus genomes which allowed the expression of small T antigen. We conclude that polyomavirus small T antigen has a (probably indirect) role in replication.
D000818:Animals; D000952:Antigens, Polyomavirus Transforming; D000957:Antigens, Viral, Tumor / Q000502:physiology*; D002460:Cell Line; D004261:DNA Replication; D005814:Genes, Viral; D051379:Mice; D009856:Oncogene Proteins, Viral / Q000502:physiology*; D011120:Polyomavirus / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D014162:Transfection; D014779:Virus Replication
D000952:Antigens, Polyomavirus Transforming; D000957:Antigens, Viral, Tumor; D009856:Oncogene Proteins, Viral