title: Site and stage specific action of endogenous nuclease and micrococcal nuclease on histone genes of sea urchin embryos.
abstract: The early histone genes of sea urchin embryos are expressed exclusively during cleavage stages of embryogenesis. The chromatin containing these genes was examined by nuclease sensitivity. An endogenous nuclease active during cleavage, produces 1300-bp segments containing early histone genes. The cutting sites have been mapped; there are very sensitive sites close to the cap site for H1, H2A, H2B, and H4. Chromatin obtained from embryos of later stages, when the genes are not expressed, do not display this pattern of nuclease sensitivity. Micrococcal nuclease produces nucleosomes that contain histone genes when used with nuclei from later stages, but not with nuclei from cleavage stages.
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000502:physiology; D002843:Chromatin / Q000648:ultrastructure*; D002970:Cleavage Stage, Ovum / Q000502:physiology; D004720:Endonucleases / Q000378:metabolism; D005775:Gastrula / Q000502:physiology; D005786:Gene Expression Regulation; D005796:Genes; D006657:Histones / Q000235:genetics*; D008836:Micrococcal Nuclease; D009707:Nucleosomes / Q000648:ultrastructure; D012617:Sea Urchins / Q000196:embryology* / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D002843:Chromatin; D006657:Histones; D009707:Nucleosomes; D004720:Endonucleases; D008836:Micrococcal Nuclease