Diazepam inhibits potassium-induced aldosterone secretion in adrenal glomerulosa cell.
In an attempt to elucidate a possible role of peripheral benzodiazepine receptor in adrenal glomerulosa cell, effect of diazepam on potassium-induced aldosterone secretion was studied using isolated bovine adrenal glomerulosa cell. Diazepam inhibited aldosterone secretion stimulated by 8mM potassium in a dose dependent manner. The ID50 was approximately 14 nM. Although diazepam inhibited potassium action effectively, forskolin-induced aldosterone secretion was not affected by diazepam. These results indicate that peripheral benzodiazepine receptor may have an active role in regulating aldosterone secretion. The voltage dependent calcium channel may be a possible site of benzodiazepine action in this tissue.
D000311:Adrenal Glands / Q000378:metabolism*; D000450:Aldosterone / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D005576:Colforsin / Q000494:pharmacology; D003975:Diazepam / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D011188:Potassium / Q000494:pharmacology; D011963:Receptors, GABA-A / Q000187:drug effects / Q000502:physiology*; D012636:Secretory Rate / Q000187:drug effects
D011963:Receptors, GABA-A; D005576:Colforsin; D000450:Aldosterone; D003975:Diazepam; D011188:Potassium