title: Susceptibility of Neisseria gonorrhoeae DNA to cleavage by restriction endonuclease KpnI.
abstract: We compared the susceptibility of Escherichia coli and Neisseria gonorrhoeae DNA to cleavage by KpnI and found that KpnI has a lower affinity for gonococcal DNA. Site-specific methylation is suggested as the cause of this altered affinity.
mesh_terms: D004248:DNA (Cytosine-5-)-Methyltransferases / Q000378:metabolism; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D004269:DNA, Bacterial / Q000378:metabolism*; D015252:Deoxyribonucleases, Type II Site-Specific; D004926:Escherichia coli / Q000235:genetics; D009344:Neisseria gonorrhoeae / Q000235:genetics*; D010957:Plasmids
chemical_list: D004269:DNA, Bacterial; D004248:DNA (Cytosine-5-)-Methyltransferases; D004262:DNA Restriction Enzymes; D015252:Deoxyribonucleases, Type II Site-Specific; C029312:GGTACC-specific type II deoxyribonucleases