title: Structural analysis of a new GC-specific insertion element IS186.
abstract: A new insertion sequence, IS186, has been identified from Escherichia coli and sequenced. It contains 1336 base pairs with a terminal inverted repeat of 22 nucleotides. A long open reading frame, from an ATG codon at position 55 to a TAG termination codon at 1327, could code for a polypeptide of 424 amino acids. This element recognizes GC-rich regions as target sites for insertion.
mesh_terms: D001482:Base Composition; D001483:Base Sequence; D003062:Codon; D004247:DNA / Q000235:genetics; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D012367:RNA, Viral / Q000235:genetics; D012412:Rubella virus / Q000235:genetics
chemical_list: D003062:Codon; D004251:DNA Transposable Elements; D012367:RNA, Viral; D004247:DNA