A signal sequence is required for the functions of the signal recognition particle.
It is shown that removal of the signal sequence of carp preproinsulin by gene technology abolishes the interaction between the polypeptide chain and the signal recognition particle with respect to both its functions: the inhibition of translation by the signal recognition particle in the absence of microsomal membranes, and the reconstitution of the protein translocation competence of high-salt inactivated microsomes. It is concluded that a signal sequence is absolutely required for the functions of the signal recognition particle.
D000818:Animals; D002347:Carps; D002474:Cell-Free System; D004285:Dogs; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D005818:Genetic Engineering; D007328:Insulin; D011384:Proinsulin / Q000378:metabolism; D014176:Protein Biosynthesis; D011498:Protein Precursors / Q000378:metabolism; D021382:Protein Sorting Signals / Q000378:metabolism*; D012261:Ribonucleoproteins / Q000378:metabolism*; D018271:Signal Recognition Particle; D014158:Transcription, Genetic
D007328:Insulin; D011498:Protein Precursors; D021382:Protein Sorting Signals; D012261:Ribonucleoproteins; D018271:Signal Recognition Particle; C013737:preproinsulin; D011384:Proinsulin