Differences in the properties of bovine brain benzodiazepine receptors in the cerebellum and hippocampus revealed after reduction of disulphide bonds.
Treatment of bovine brain cell membranes with dithiothreitol decreases the affinity without changing binding capacity for [3H]flunitrazepam in the cerebellum, hippocampus and frontal cortex. Specific binding of beta-[3H]carboline-3-carboxylic acid ethyl ester in the cerebellum and frontal cortex was influenced by dithiothreitol in a similar manner. However, in the hippocampus the binding of this inverse agonist was modified by dithiothreitol in another way: a significant (25%) decrease of binding capacity, or (and) appearance of heterogeneity of binding sites was detected. Sensitivity of benzodiazepine receptors to gamma-aminobutyric acid (GABA) stimulation was not changed by dithiothreitol.
D000818:Animals; D001923:Brain Chemistry / Q000187:drug effects*; D002243:Carbolines / Q000378:metabolism; D002417:Cattle; D002531:Cerebellum / Q000032:analysis; D002540:Cerebral Cortex / Q000032:analysis; D004229:Dithiothreitol / Q000494:pharmacology*; D005445:Flunitrazepam / Q000378:metabolism; D006624:Hippocampus / Q000032:analysis; D010084:Oxidation-Reduction; D011963:Receptors, GABA-A / Q000032:analysis / Q000187:drug effects* / Q000378:metabolism
D002243:Carbolines; D011963:Receptors, GABA-A; D005445:Flunitrazepam; C024915:beta-carboline-3-carboxylic acid ethyl ester; D004229:Dithiothreitol