[Intervention of the calcium-calmodulin system in the release of growth hormone induced by somatocrinin].
This study was carried out to evaluate the role of the calcium-calmodulin system in cellular mediation of somatocrinin action by cells producing growth hormone. We compared GH release to intra- and extracellular cAMP levels, after incubation of pituitary cells in monolayer culture with somatocrinin alone or somatocrinin associated with exogenous calmodulin, or specific calmodulin inhibitor (W13). Calmodulin (10(-6) M) decreases somatocrinin - induced cAMP levels, maintaining elevated GH levels. W13 (10(-5) M) increases somatocrinin - induced cAMP and GH levels. Thus GH release induced by somatocrinin is modulated by two interrelated systems, adenylcyclase - cAMP system and Ca++-calmodulin system.
D000818:Animals; D002118:Calcium / Q000502:physiology*; D002147:Calmodulin / Q000502:physiology*; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism; D005260:Female; D013006:Growth Hormone / Q000378:metabolism*; D013007:Growth Hormone-Releasing Hormone / Q000494:pharmacology*; D010446:Peptide Fragments / Q000494:pharmacology*; D010903:Pituitary Gland, Anterior / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013449:Sulfonamides / Q000494:pharmacology
D002147:Calmodulin; D010446:Peptide Fragments; D013449:Sulfonamides; C033772:N-(4-aminobutyl)-5-chloro-2-naphthalenesulfonamide; D013006:Growth Hormone; D013007:Growth Hormone-Releasing Hormone; D000242:Cyclic AMP; D002118:Calcium
Intervention du système calcium-calmoduline dans la libération de l'hormone de croissance provoquée par la somatocrinine.