Immunogold electron-microscopic localisation of calpain I in skeletal muscle of rats.
By using a double-affinity-purified first antibody and colloidal gold-conjugated second antibody, it is shown that calpain I (a cysteine proteinase activated by micromolar concentrations of Ca2+) has a predominant intracellular location in the I-band region of the extensor digitorum longus (EDL) muscle of the rat, but is not exclusively associated with the Z-line.
D000818:Animals; D002154:Calpain / Q000032:analysis* / Q000276:immunology; D006046:Gold; D008297:Male; D009132:Muscles / Q000201:enzymology* / Q000648:ultrastructure; D051381:Rats; D011919:Rats, Inbred Strains
D006046:Gold; D002154:Calpain