Localization and characterization of specific receptors for atrial natriuretic factor in the ciliary processes of the eye.
By light and electron microscope radioautography in vivo, competitive binding sites for 125I-Arg 101-Tyr 126 atrial natriuretic factor were localized mostly on the "pigmented" epithelium of the rat ciliary process. Further investigation using isolated ciliary processes from rabbits demonstrated the presence of specific receptors for 125I-atrial natriuretic factor. In addition, synthetic atrial natriuretic factor inhibited basal and stimulated adenylate cyclase activity. These results demonstrate for the first time the presence of specific receptors for atrial natriuretic factor in the ciliary processes which are negatively coupled to adenylate cyclase. The possible role of this peptide in the control of intraocular pressure is suggested.
D000262:Adenylyl Cyclases / Q000378:metabolism; D000818:Animals; D009320:Atrial Natriuretic Factor / Q000378:metabolism; D001345:Autoradiography; D002924:Ciliary Body / Q000201:enzymology / Q000378:metabolism* / Q000648:ultrastructure; D005123:Eye / Q000378:metabolism; D005260:Female; D007457:Iodine Radioisotopes; D008854:Microscopy, Electron; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains; D017461:Receptors, Atrial Natriuretic Factor; D011956:Receptors, Cell Surface / Q000378:metabolism*; D014018:Tissue Distribution
D007457:Iodine Radioisotopes; D011956:Receptors, Cell Surface; D009320:Atrial Natriuretic Factor; D000262:Adenylyl Cyclases; D017461:Receptors, Atrial Natriuretic Factor