Redox changes in coenzyme Q in the millisecond time range: an approach using rapid quenching and high-performance liquid chromatography.
We have combined a rapid-quenching protocol with HPLC analysis to measure the kinetics of reduction of coenzyme Q in a mitochondrial enzyme complex. The method has a time resolution of several milliseconds and will readily measure 1-20 nmol of the Q derivatives under investigation. By changing the HPLC solvent, either Q6 or Q10 can be studied.
D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation*; D004578:Electron Spin Resonance Spectroscopy; D014450:Electron Transport Complex III; D066298:In Vitro Techniques; D007700:Kinetics; D008929:Mitochondria, Heart / Q000378:metabolism; D009097:Multienzyme Complexes / Q000378:metabolism; D010084:Oxidation-Reduction; D011808:Quinone Reductases / Q000378:metabolism; D012997:Solvents; D013997:Time Factors; D014451:Ubiquinone / Q000378:metabolism*
D009097:Multienzyme Complexes; D012997:Solvents; D014451:Ubiquinone; D011808:Quinone Reductases; D014450:Electron Transport Complex III