Defective chemiluminescence response in differentiated HL60 cells due to impaired degranulation.
In the presence of dimethyl sulfoxide, the promyelocytic leukemic cell line, HL60, differentiates into apparently mature polymorphonuclear leukocytes. When correlating the superoxide production from HL60 cells with the number of phagocytozing and NBT-positive cells, no difference was observed in comparison with normal peripheral blood leukocytes. In contrast, the luminol-dependent chemiluminescence was greatly impaired in the differentiated HL60 cells. Analysis of degranulation, i.e., release of myeloperoxidase and N-acetyl-beta-glucosaminidase- and myeloperoxidase-mediated iodination by HL60 cells, suggested that the defective chemiluminescence response observed in HL60 cells may be due to impaired release of myeloperoxidase from azurophilic granulae. This may lead to impaired microbicidal activity in these cells.
D002454:Cell Differentiation; D002460:Cell Line; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000473:pathology*; D008163:Luminescent Measurements; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects; D009580:Nitroblue Tetrazolium; D009195:Peroxidase / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D013481:Superoxides; D009580:Nitroblue Tetrazolium; D009195:Peroxidase; D013755:Tetradecanoylphorbol Acetate