Nucleic acid hybridization for detection of herpes viruses in clinical specimens.
A diagnostic hybridization assay for detecting varicella zoster virus (VZV), herpes simplex virus (HSV), and Epstein-Barr virus (EBV) in different clinical specimens was developed using cloned viral DNAs as probes. All probes detected at least 5 pg of homologous DNA and did not cross-react with other viral or cellular DNA. Results of cell culture, serology, and DNA assay were highly concordant. Using a simple standardized protocol for preparation of specimens, hybridization, and washing procedures, this sensitive and specific assay appears to be useful for screening clinical specimens and may be helpful in confirming the serological diagnosis of HSV encephalitis and persistent EBV infections or EBV-associated diseases.
D000328:Adult; D000368:Aged; D002644:Chickenpox / Q000175:diagnosis; D002648:Child; D002675:Child, Preschool; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000032:analysis*; D004660:Encephalitis / Q000175:diagnosis; D005260:Female; D006561:Herpes Simplex / Q000175:diagnosis; D006562:Herpes Zoster / Q000175:diagnosis; D006564:Herpesviridae / Q000235:genetics / Q000302:isolation & purification*; D006566:Herpesviridae Infections / Q000175:diagnosis*; D014645:Herpesvirus 3, Human / Q000235:genetics / Q000302:isolation & purification; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D008297:Male; D008875:Middle Aged; D009693:Nucleic Acid Hybridization; D018139:Simplexvirus / Q000235:genetics / Q000302:isolation & purification
D004279:DNA, Viral