title: The use of cyclic AMP efflux studies in attempts to determine the effects of morphine on cyclic AMP formation in striatal slices.
abstract: The effect of morphine and naloxone on basal and forskolin-stimulated efflux of cyclic AMP from rat striatal slices was examined. Neither morphine nor naloxone had any consistent effect on the basal efflux of cyclic AMP. Forskolin produced a time and dose-dependent enhancement of cyclic AMP efflux. Neither morphine nor naloxone affected this forskolin-enhanced release. These results suggest that measurements of cyclic AMP released from brain slices do not accurately reflect effects on adenylate cyclase inhibition by opiates.
mesh_terms: D015647:2,3,4,5-Tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benzazepine; D000067956:Adenylyl Cyclase Inhibitors; D000818:Animals; D001058:Apomorphine / Q000494:pharmacology; D001552:Benzazepines / Q000494:pharmacology; D005576:Colforsin / Q000494:pharmacology; D003342:Corpus Striatum / Q000187:drug effects* / Q000378:metabolism; D000242:Cyclic AMP / Q000096:biosynthesis*; D004347:Drug Interactions; D066298:In Vitro Techniques; D009020:Morphine / Q000494:pharmacology*; D009270:Naloxone / Q000494:pharmacology; D051381:Rats
chemical_list: D000067956:Adenylyl Cyclase Inhibitors; D001552:Benzazepines; D005576:Colforsin; D009270:Naloxone; D015647:2,3,4,5-Tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benzazepine; D009020:Morphine; D000242:Cyclic AMP; D001058:Apomorphine