"In vitro" method of assembling a synthetic gene.
Without prior in vitro enzymatic ligation a DNA duplex was assembled successfully by directly transforming competent cells with a mixture containing six synthetic complimentary oligodeoxyribonucleotides and a linearized plasmid. One out of 100 transformants was positive in colony hybridization with one of the synthetic fragment probe. The sequence of the DNA duplex inserted into the plasmid was confirmed by dideoxy sequencing method.
D001483:Base Sequence; D004247:DNA / Q000138:chemical synthesis*; D004262:DNA Restriction Enzymes / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D009838:Oligodeoxyribonucleotides / Q000378:metabolism; D010957:Plasmids
D009838:Oligodeoxyribonucleotides; D004247:DNA; D004262:DNA Restriction Enzymes