[Two loci in the genome of the Pseudomonas aeruginosa transposable phage B39 which affect the integration process. II. Mapping of the pdeX and pdeY loci by restriction and heteroduplex analysis].
The coordinate function of two loci - pdeX and pdeY - in the genome of a transposable phage (TP) provides the phage function pde+ (good growth on bacteria with Rms163 plasmid). When these two loci in hybrid phages originate from different TP, some of the hybrid phages have Pde- phenotype. To localize pdeX and pdeY, the structure of hybrid TP genomes with Pde+ and Pde- phenotype obtained in crosses between B39ts+ and PH132 were studied using restriction and heteroduplex analysis. On the basis of data obtained, pdeX and pdeY were mapped in 2.85-6.4 and 6.4-16 kbp regions, respectively.
D001435:Bacteriophages / Q000235:genetics*; D002874:Chromosome Mapping; D004262:DNA Restriction Enzymes; D004251:DNA Transposable Elements; D004279:DNA, Viral / Q000235:genetics; D005814:Genes, Viral; D009692:Nucleic Acid Heteroduplexes; D010641:Phenotype; D011550:Pseudomonas aeruginosa
D004251:DNA Transposable Elements; D004279:DNA, Viral; D009692:Nucleic Acid Heteroduplexes; D004262:DNA Restriction Enzymes
Dva lokusa v genome faga-transpozona B39 Pseudomonas aeruginosa, vliiaiushchie na protsess integratsii. Soobshchenie II. Opredelenie polozheniia lokusov pdeX i pdeY metodami restriktsionnogo i geterodupleksnogo analizov.