Increased release of hydrogen peroxide (H2O2) and superoxide anion (O-2) by murine macrophages in vitro after cis-platin treatment.
When murine macrophage (M phi) monolayers are treated with cis-Platin (10 micrograms or 5 micrograms/ml) for 30 min, 1, 2, 4, 8 and 24 h a significant increase in the release of H2O2 and O-2 by M phi is observed. The release of H2O2 and O-2 was comparatively much more when M phi were treated with 5 micrograms/ml cis-Platin than 10 micrograms/ml. However, it is observed that 4 h cis-Platin treatment results in comparative inhibition in the release of H2O2 and O-2. Further, we compared the release of H2O2 and O-2 by M phi treated with cis-Platin, LPS and PMA.
D000818:Animals; D002478:Cells, Cultured; D002945:Cisplatin / Q000494:pharmacology*; D003574:Cytochrome c Group / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism*; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D010084:Oxidation-Reduction; D013481:Superoxides / Q000378:metabolism*
D003574:Cytochrome c Group; D013481:Superoxides; D006861:Hydrogen Peroxide; D002945:Cisplatin