Seasonal variations in the stability of monoamines and their metabolites in perchloric acid as measured by high-performance liquid chromatography.
The stability in acid medium of dopamine, dihydroxyphenylacetic acid (DO-PAC), homovanillic acid (HVA), serotonin (5-HT) and 5-hydroxyindoleacetic acid (5-HIAA) was investigated. The stability of 5-HT and 5-HIAA was poor, but could greatly be improved by the addition of sodium bisulphite and disodium edetate. Under these conditions, dopamine, DOPAC, HVA, 5-HT and 5-HIAA showed good stability over 24 h at room temperature throughout the year when stored in capped vials. In uncapped vials, the stability of 5-HT and 5-HIAA was reasonable during the winter months, but was poor during the summer months.
D001679:Biogenic Amines / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D004355:Drug Stability; D008027:Light; D010472:Perchlorates; D012621:Seasons; D013997:Time Factors
D001679:Biogenic Amines; D010472:Perchlorates