Rapid physical mapping by transposon Tn5 mutagenesis to localize the cloned yeast ILV2 gene.
A rapid method for Tn5 mutagenesis of cloned genes on multicopy plasmids was used to map a yeast ILV2 mutant allele encoding a sulfometuron methyl-resistant acetolactate synthase. Twenty-one of 40 independent Tn5 insertions were within the 5.6-kilobase-pair cloned segment. Of these, seven adjacent transposition events inactivated the sulfometuron methyl resistance determinant, localizing the ILV2 gene to a minimum 1.4-kilobase-pair region.
D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D005800:Genes, Fungal; D009154:Mutation; D010957:Plasmids; D015003:Yeasts / Q000235:genetics*
D004251:DNA Transposable Elements