title: Effects of endogenous and exogenous opioids on splenic natural killer cell activity.
abstract: Previous work from our own and other laboratories has shown that electroshock-induced neurohormonal changes in rodents could modify host-tumor interactions by both increasing the frequency and growth rate of transplanted tumors and decreasing the elimination rate of a radiolabelled natural killer (NK) cell sensitive tumor. To test whether such neurohormonal changes could affect NK activity we subjected mice to tail electrode shock (TES) and examined in vitro splenic NK activity. We found that between 30 and 60 min after TES there is a significant but transient suppression of their splenic NK activity. To determine whether TES-induced endogenous opioids might be involved in this suppression mice were given intraperitoneal injections of the opioid antagonists naloxone or naltrexone before or at the end of the TES session. These drugs prevented NK suppression. In a further test of the hypothesis that opioids alter NK activity mice were given a single intraperitoneal injection of morphine or [D-Ala2-Met5]-beta-endorphin, a relatively stable analogue of beta-endorphin, an endogenous opioid. Contrary to expectations these opioids enhanced splenic NK activity. Our interpretation of these results is that shock-induced NK suppression may not be mediated by endogenous opioids and that the effects of naloxone and naltrexone on NK activity may not be related to their opioid antagonist properties. On the contrary, opioids may participate in a homeostatic rebound from suppression mediated by other neurohormonal mechanisms activated during TES.
mesh_terms: D000324:Adrenocorticotropic Hormone / Q000378:metabolism / Q000502:physiology*; D000818:Animals; D003601:Cytotoxicity Tests, Immunologic; D004558:Electric Stimulation; D004723:Endorphins / Q000378:metabolism / Q000502:physiology*; D005260:Female; D007274:Injections, Intraperitoneal; D007694:Killer Cells, Natural / Q000187:drug effects* / Q000502:physiology; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009020:Morphine / Q000008:administration & dosage / Q000494:pharmacology*; D009270:Naloxone / Q000494:pharmacology*; D009271:Naltrexone / Q000494:pharmacology; D011070:Poly I-C / Q000008:administration & dosage / Q000494:pharmacology; D013154:Spleen / Q000166:cytology*; D001615:beta-Endorphin
chemical_list: D004723:Endorphins; D009270:Naloxone; D009271:Naltrexone; D001615:beta-Endorphin; D009020:Morphine; D000324:Adrenocorticotropic Hormone; D011070:Poly I-C