Superoxide anion generation during airway anaphylaxis.
Extracellular release of superoxide anion (O-2) during in vitro anaphylaxis in guinea pig trachealis was found to exhibit both a time- and a concentration-dependent generation of O-2. Trachealis O-2 release was unaffected by treatment with diphenhydramine HCl (10(-5) M, 10(-6) M). While indomethacin (10(-5) M) augmented anaphylaxis tension it did not enhance O-2 release. However, the semiselective SRS-A antagonist FPL 55712 (10(-5) M) resulted in essentially complete inhibition of O-2 generation. These data indicate that O-2 generation occurring during acute airway anaphylaxis is associated with the activation of SRS-A products.
D000707:Anaphylaxis / Q000378:metabolism*; D000818:Animals; D005110:Extracellular Space / Q000378:metabolism; D006168:Guinea Pigs; D008297:Male; D013189:SRS-A / Q000494:pharmacology; D013481:Superoxides / Q000378:metabolism*; D014132:Trachea / Q000378:metabolism*
D013189:SRS-A; D013481:Superoxides