Purification of opioid-binding materials from rat brain.
Opiate receptors were solubilized from rat brain with digitonin and treated with an affinity resin, AH-Sepharose coupled with [D-Ala2, Leu5]enkephalin. The eluted materials from the resin with the opioid peptide had opioid-binding activity. Based on the protein content of the purified materials, 450-fold purification over the solubilized receptors was achieved. Analyses by gel electrophoresis revealed that the purified materials were rich in one polypeptide with a molecular weight of 62,000.
D000818:Animals; D001923:Brain Chemistry; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D004743:Enkephalin, Leucine / Q000031:analogs & derivatives / Q000378:metabolism; D016308:Enkephalin, Leucine-2-Alanine; D008970:Molecular Weight; D051381:Rats; D011957:Receptors, Opioid / Q000302:isolation & purification* / Q000378:metabolism
D011957:Receptors, Opioid; D004743:Enkephalin, Leucine; D016308:Enkephalin, Leucine-2-Alanine