Fructose 2,6-bisphosphate and the control of glycolysis in bovine spermatozoa.
Epididymal bovine sperm contain fructose-1,6-bisphosphatase activity which is inhibited by AMP and by fructose 2,6-bisphosphate. Sperm phosphofructokinase displays kinetic characteristics that are typical of the F-type and it is stimulated by fructose 2,6-bisphosphate. The concentration of sperm fructose 2,6-bisphosphate remained unaffected at 1-2 microM when the glycolytic rate was either increased by glucose, caffeine or antimycin, or decreased by alpha-chlorohydrin or 6-chloro-6-deoxyglucose.
D000818:Animals; D002417:Cattle; D006597:Fructose-Bisphosphatase / Q000378:metabolism; D005635:Fructosediphosphates / Q000378:metabolism*; D006019:Glycolysis; D006598:Hexosediphosphates / Q000378:metabolism*; D007700:Kinetics; D007773:Lactates / Q000378:metabolism; D008297:Male; D010732:Phosphofructokinase-1 / Q000378:metabolism; D013094:Spermatozoa / Q000378:metabolism*
D005635:Fructosediphosphates; D006598:Hexosediphosphates; D007773:Lactates; C027652:fructose 2,6-diphosphate; D010732:Phosphofructokinase-1; D006597:Fructose-Bisphosphatase