Structure of the extra-membranous domain of the beta-subunit of (Na,K)-ATPase revealed by the sequences of its tryptic peptides.
Membrane bound dog kidney (Na,K)-ATPase was digested with trypsin. The peptides that were recovered in the supernatant were purified and sequenced. By comparing these results with the sequence of alpha- and human beta-subunits, the location of each of the peptides could be allotted. Both accessibility to trypsin and the facility of release into the water phase indicated that these peptides were derived from the exposed surface of the intact enzyme. The sequence, GXGXXG, reported in the Torpedo californica beta-subunit [(1986) FEBS Lett. 196, 315-319] was likely a mere coincidence with the sequence of the dinucleotide-binding site, since the last glycine was replaced by proline in the sequence of the dog beta-subunit. A disulfide bridge was found within a peptide derived from the beta-subunit. A possible model for the beta-subunit structure is proposed.
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D002462:Cell Membrane / Q000201:enzymology; D002851:Chromatography, High Pressure Liquid; D015226:Dinucleoside Phosphates; D004285:Dogs; D005033:Ethylmaleimide; D007668:Kidney / Q000201:enzymology*; D009841:Oligonucleotides / Q000378:metabolism; D010446:Peptide Fragments / Q000302:isolation & purification*; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D014101:Torpedo; D014357:Trypsin
D015226:Dinucleoside Phosphates; D009841:Oligonucleotides; D010446:Peptide Fragments; D014357:Trypsin; D000254:Sodium-Potassium-Exchanging ATPase; D005033:Ethylmaleimide