Studies on herpes simplex virus type 1 glycoproteins using monoclonal antibodies.
Monoclonal antibodies against herpes simplex virus type 1 glycoproteins were isolated and utilized to study the synthesis and processing of glycoproteins B, C, and D (gB, gC, gD, respectively). Monoclonal antibodies against both gB and gD had higher virus-neutralizing activity when compared to that of gC. Differences among these glycoproteins were observed in their time of appearance in the virus-infected cells. The presence of gD was detected at a very early stage of infection when compared to gB and gC. The localization of these glycoproteins during their synthesis and processing was studied.
D000818:Animals; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D002462:Cell Membrane / Q000032:analysis; D002478:Cells, Cultured; D004721:Endoplasmic Reticulum / Q000032:analysis; D005455:Fluorescent Antibody Technique; D006056:Golgi Apparatus / Q000032:analysis; D006801:Humans; D009685:Nuclear Envelope / Q000032:analysis; D018139:Simplexvirus / Q000032:analysis / Q000254:growth & development / Q000276:immunology / Q000378:metabolism*; D013997:Time Factors; D014759:Viral Envelope Proteins / Q000032:analysis / Q000096:biosynthesis / Q000276:immunology / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D014759:Viral Envelope Proteins; C040495:glycoprotein B, Simplexvirus; C039447:glycoprotein D, Human herpesvirus 1; C038535:glycoprotein gC, herpes simplex virus type 1