A pH-metrical method of determining glucose-6-phosphatase activity.
A simple, rapid, and reproducible method of determining glucose-6-phosphatase activity is described. The glucose 6-phosphate hydrolysis is accompanied by the disappearance of the protons from the medium owing to a phosphate species pK change from 6.1 (in glucose 6-phosphate) to 6.9 (in inorganic phosphate). Alkalization is registered by a pH meter with a recorder. The method described in this paper may be used in routine determinations of glucose-6-phosphatase activity.
D000818:Animals; D005952:Glucose-6-Phosphatase / Q000032:analysis*; D019298:Glucose-6-Phosphate; D005958:Glucosephosphates; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008297:Male; D008722:Methods; D008862:Microsomes, Liver / Q000201:enzymology; D051381:Rats
D005958:Glucosephosphates; D019298:Glucose-6-Phosphate; D005952:Glucose-6-Phosphatase