Effects of diethyldithiocarbamate and N-methyl-N-dithiocarboxyglucamine on murine hepatic cadmium-metallothionein in vitro.
A study was made of the effects of diethyldithiocarbamate (DDTC) and N-methyl-N-dithiocarboxyglucamine (MDCG) on partially purified cadmium-metallothionein (Cd-MT) in vitro obtained from livers of mice previously injected with CdCl2 containing 109CdCl2. Analytical Sephadex G-75 gel filtration showed that MDCG effected a time-dependent removal of Cd from MT, and 99% of the Cd was recovered as the soluble Cd(MDCG)2 complex after 24 hr of incubation. Only a portion of Cd of Cd-MT was complexed by DDTC after 24 hr of incubation; 44% remained as Cd-MT, and the net loss following centrifugation prior to application of the samples to the column corresponded to 56% of the Cd originally present. It was proposed that MDCG complexes the 4 g-atoms of Cd in cluster A of MT as well as the 3 g-atoms in cluster B, while DDTC complexes only the 4 g-atoms of Cd in cluster A.
D000818:Animals; D002104:Cadmium / Q000378:metabolism*; D002219:Carbamates / Q000494:pharmacology*; D004050:Ditiocarb / Q000494:pharmacology*; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism*; D008297:Male; D008668:Metallothionein / Q000378:metabolism*; D051379:Mice; D008815:Mice, Inbred Strains; D013012:Sorbitol / Q000031:analogs & derivatives*; D013113:Spin Labels; D013859:Thiocarbamates / Q000494:pharmacology*
D002219:Carbamates; D013113:Spin Labels; D013859:Thiocarbamates; D002104:Cadmium; D013012:Sorbitol; C041262:N-methyl-D-glucamine dithiocarbamate; D008668:Metallothionein; D004050:Ditiocarb