Mechanism of interferon action. Expression of vesicular stomatitis virus G gene in transfected COS cells is inhibited by interferon at the level of protein synthesis.
The effect of interferon on the expression of the vesicular stomatitis virus glycoprotein G gene was examined in simian COS cells transfected with the expression vector pSVGL containing the G gene under the control of the SV40 late promoter. When COS cells were treated with interferon 24 h after transfection, the synthesis of vesicular stomatitis virus G protein was inhibited by about 80% as compared to that in untreated controls. By contrast, under the same conditions, neither the plasmid copy number nor the G gene mRNA levels were detectably affected by interferon treatment. Likewise, the synthesis of simian virus 40 large T-antigen was not inhibited by interferon treatment of transfected COS cells even though the synthesis of vesicular stomatitis virus G protein was markedly inhibited. The residual G protein synthesized in transfected, interferon-treated COS cells appeared to be normally glycosylated.
D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D005796:Genes / Q000187:drug effects*; D005814:Genes, Viral / Q000187:drug effects*; D005822:Genetic Vectors; D006801:Humans; D007370:Interferon Type I / Q000494:pharmacology*; D007668:Kidney; D008562:Membrane Glycoproteins; D010957:Plasmids; D014158:Transcription, Genetic / Q000187:drug effects*; D014162:Transfection; D014721:Vesicular stomatitis Indiana virus / Q000187:drug effects / Q000235:genetics*; D014759:Viral Envelope Proteins; D014764:Viral Proteins / Q000235:genetics*
C028391:G protein, vesicular stomatitis virus; D007370:Interferon Type I; D008562:Membrane Glycoproteins; D014759:Viral Envelope Proteins; D014764:Viral Proteins