The morphology of redox-dye-treated HbH-containing red cells: differences between cells treated with brilliant cresyl blue, methylene blue and new methylene blue.
The ultrastructure of redox-dye-treated HbH-containing red cells has been shown to be dependent on the nature of the redox dye used. Brilliant cresyl blue causes the formation of a large number of small inclusions which are invariably attached to the inner surface of the red cell membrane. Methylene blue and new methylene blue cause the formation of a much smaller number of larger inclusions only some of which appear to be membrane-bound. These ultrastructural differences are reflected in marked differences in the light microscope appearances of the supravitally-stained cells.
D000328:Adult; D004396:Coloring Agents; D004912:Erythrocytes / Q000378:metabolism* / Q000648:ultrastructure; D006447:Hemoglobin H / Q000378:metabolism*; D006455:Hemoglobins, Abnormal / Q000378:metabolism*; D006801:Humans; D002479:Inclusion Bodies / Q000648:ultrastructure; D008751:Methylene Blue / Q000031:analogs & derivatives; D010078:Oxazines; D010084:Oxidation-Reduction
D004396:Coloring Agents; D006455:Hemoglobins, Abnormal; D010078:Oxazines; C035184:new methylene blue; C006797:Brilliant Cresyl Blue; D006447:Hemoglobin H; D008751:Methylene Blue