Na+-ATPase is a different entity from the (Na+ + K+)-ATPase in rat kidney basolateral plasma membranes.
In this work, we present evidence in agreement with the hypothesis that there exist two Na+-stimulated ATPase activities in basolateral plasma membranes from rat kidney proximal tubular cells: (1) (Na+ + K+)-ATPase activity, which is inhibited by ouabain and by treating the membranes with trypsin, is insensitive to furosemide and reaches maximal activity upon treatment with SDS at an SDS/protein ratio of 1.6; (2) the Na+-ATPase activity, which is insensitive to ouabain and to trypsin treatment, is inhibited by furosemide and reaches maximal activity upon treatment with SDS at an SDS/protein ratio of 0.4.
D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000818:Animals; D027682:Cation Transport Proteins; D002462:Cell Membrane / Q000201:enzymology; D005665:Furosemide / Q000494:pharmacology; D007687:Kidney Tubules, Proximal / Q000201:enzymology*; D010042:Ouabain / Q000494:pharmacology; D051381:Rats; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D012974:Sodium Iodide / Q000494:pharmacology; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors / Q000378:metabolism; D014357:Trypsin
D027682:Cation Transport Proteins; D012967:Sodium Dodecyl Sulfate; D010042:Ouabain; D005665:Furosemide; D014357:Trypsin; D000251:Adenosine Triphosphatases; C021838:sodium-translocating ATPase; D000254:Sodium-Potassium-Exchanging ATPase; D012974:Sodium Iodide