title: The complete sequence of bluetongue virus serotype 10 segment 3 and its predicted VP3 polypeptide compared with those of BTV serotype 17.
abstract: The complete sequence of the large RNA segment 3 (L3) of bluetongue virus serotype 10 (BTV-10) has been determined from DNA copies of the viral RNA cloned in the E. coli plasmid pBR322. The L3 viral RNA is 2772 nucleotides long with a single open reading frame of 2706 nucleotides. The L3 predicted primary gene product (VP3) is 103 342 daltons and has a net charge at neutral pH of -5. The sequence of the L3 RNA species differs by 126 point mutations from that of BTV-17 (i.e., 95.5% homology; see M. Purdy, J. Petre and P. Roy, J. Virol. 51, 754-759, 1984). The predicted L3 primary gene products of the two viruses differ by 9 amino acids. These differences correspond to 9 point mutations and represent 0.15% of the sites where nucleotide substitution could cause an amino acid change. By contrast, another 114 point mutations in the genome correspond to 6.5% of the available sites where nucleotide substitutions could be silent (i.e., where a nucleotide substitution may not cause an amino acid change). Three point mutations are in the 3' non-coding region of the RNA species. The quantitative differences between the coding and silent mutations are interpreted as representing the result of gene product conservation.
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D001483:Base Sequence; D001820:Bluetongue virus / Q000145:classification / Q000235:genetics* / Q000276:immunology; D003001:Cloning, Molecular; D004247:DNA; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D005814:Genes, Viral; D009154:Mutation; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000032:analysis / Q000235:genetics*; D012087:Reoviridae / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D012703:Serotyping; D014764:Viral Proteins / Q000032:analysis / Q000235:genetics*; D015678:Viral Structural Proteins
chemical_list: D000596:Amino Acids; D004279:DNA, Viral; D012367:RNA, Viral; D014764:Viral Proteins; D015678:Viral Structural Proteins; D004247:DNA