Characterization of the OCT plasmid encoding alkane oxidation and mercury resistance in Pseudomonas putida.
Transformation of Pseudomonas putida and analysis for plasmid DNA revealed that both n-alkane oxidation and mercury resistance are encoded on a single 220-megadalton OCT plasmid molecule. Derivatives of OCT having lost the mercury resistance function could be readily isolated and contained a smaller plasmid estimated to be 170 megadaltons. The results show that segregation of the mercury resistance property occurs not by loss of a separate MER plasmid as previously thought but by a deletion in the OCT plasmid.
D000473:Alkanes / Q000378:metabolism*; D002872:Chromosome Deletion; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004352:Drug Resistance, Microbial; D008628:Mercury / Q000494:pharmacology*; D010084:Oxidation-Reduction; D010957:Plasmids; D011549:Pseudomonas / Q000235:genetics*; D014170:Transformation, Genetic
D000473:Alkanes; D004262:DNA Restriction Enzymes; D008628:Mercury