Mast-cell products and heparin stimulate the production of mononuclear-cell factor by cultured human monocyte/macrophages.
Purified mast cells derived from rat peritoneal fluids and dog mastocytomas were extracted with 1 M-NaCl and sonication techniques. The mast-cell products increased the production of mononuclear cell factor from human peripheral blood mononuclear cells in culture, as judged by the enhanced stimulation of prostaglandin E (2-5 fold) and collagenase (3-11-fold) production by cultured adherent synovial cells. Heparin alone (1-10 micrograms/ml) induced a similar stimulation of mononuclear-cell-factor production by monocyte cultures, whereas histamine (1-10 micrograms/ml) had no effect. The stimulatory effect of mast-cell products and heparin represented a direct effect on mononuclear cells; they did not potentiate the effect of monokine on the synovial cells. These results suggest that mast-cell-macrophage interactions may play a significant role in the pathogenesis of inflammation and connective-tissue degradation.
D002478:Cells, Cultured; D006493:Heparin / Q000494:pharmacology*; D006632:Histamine / Q000494:pharmacology; D006801:Humans; D007962:Leukocytes / Q000187:drug effects / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism; D008407:Mast Cells / Q000378:metabolism*; D003012:Microbial Collagenase / Q000378:metabolism; D015846:Monokines; D012157:Mononuclear Phagocyte System / Q000378:metabolism; D011458:Prostaglandins E / Q000378:metabolism; D014176:Protein Biosynthesis
D015846:Monokines; D011458:Prostaglandins E; D006632:Histamine; D006493:Heparin; D003012:Microbial Collagenase