Rabbit kidney cells abortively infected with human cytomegalovirus are arrested in mitotic phase.
In rabbit kidney epithelial cells (RK13) abortively infected with human cytomegalovirus (HCMV), DNA synthesis at 1 or 2 days post-infection was enhanced 4 to 5 fold, compared to mock-infected cells. DNA analysis by isopycnic centrifugation revealed that the DNA newly synthesized in the virus infected RK13 cells was of cellular origin. HCMV infection also caused a marked increase in the mitotic activity of RK13 cells. When semi-confluent RK13 cells were infected more than 20 per cent of cells demonstrated mitosis at 72 hours post-infection although the rate of cell growth was considerably reduced compared to that of uninfected cells. The most frequent chromosomal change observed was fragmentation although other aberrations, gap, break, deletion etc. occurred also. Two immediate-early viral polypeptides with apparent molecular weights 72,000 (72K) and 76,000 (76K) daltons were produced in both RK13 cells and human embryonic lung cells (HEL) by 3 hours post-infection. Synthesis of the 76K polypeptide was greater than that of the 72K polypeptide in non-permissive RK13 cells whereas the reverse occurred in permissive HEL cells. Furthermore, of three early polypeptides which were expressed in productively infected HEL cells two, 88K and 80K, were not detected in abortively infected RK13 cells. These results suggest that the arrest in mitosis of the abortively infected RK13 cells and the subsequent chromosomal changes are associated with the altered expression of immediate-early or early virus functions in these cells.
D000818:Animals; D002460:Cell Line; D002472:Cell Transformation, Viral; D002869:Chromosome Aberrations; D003587:Cytomegalovirus / Q000235:genetics*; D004247:DNA / Q000302:isolation & purification; D004261:DNA Replication; D006801:Humans; D007621:Karyotyping; D007668:Kidney / Q000166:cytology*; D008168:Lung / Q000196:embryology; D008938:Mitosis; D008940:Mitotic Index; D011817:Rabbits
D004247:DNA