title: Chemical modification of arginine residues of porcine muscle acylphosphatase.
abstract: Acylphosphatase (acylphosphate phosphohydrolase, EC 3.6.1.7) from porcine skeletal muscle is inactivated by phenylglyoxal following pseudo-first-order kinetics. The dependence of the apparent first-order rate constant for inactivation on the phenylglyoxal concentration shows that the inactivation is also first order with respect to the reagent concentration. Among the competitive inhibitors for the enzyme examined, inorganic phosphate and ATP almost completely, and Cl- partially, protect the enzyme against the inactivation. The dissociation constants for inorganic phosphate and ATP determined from protection experiments by these inhibitors agree well with those from inhibition experiments by them. These results support the idea that the modification occurs at the phosphate-binding site. The amino-acid analysis reveals the lack of reaction at residues other than arginine. Circular dichroism spectra of the modified enzymes show that the inactivation seems not to be due to denaturation of the enzyme resulting from the modification of the non-essential arginine residues. The relationship between the loss of the enzyme activity and the number of arginine residues modified in the presence and absence of ATP shows that one arginine residue is possibly responsible for the inactivation of acylphosphatase.
mesh_terms: D017766:Acid Anhydride Hydrolases; D000447:Aldehydes / Q000494:pharmacology*; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001120:Arginine; D007700:Kinetics; D009132:Muscles / Q000201:enzymology*; D010446:Peptide Fragments / Q000032:analysis; D010658:Phenylglyoxal / Q000494:pharmacology*; D010744:Phosphoric Monoester Hydrolases / Q000037:antagonists & inhibitors*; D011485:Protein Binding; D013552:Swine; D000097024:Acylphosphatase
chemical_list: D017766:Acid Anhydride Hydrolases; D000447:Aldehydes; D000596:Amino Acids; D001120:Arginine; D010446:Peptide Fragments; D010658:Phenylglyoxal; D010744:Phosphoric Monoester Hydrolases; D000097024:Acylphosphatase