Immunocytochemical localization of a tartrate-resistant and vanadate-sensitive acid nucleotide tri- and diphosphatase.
Purified rabbit antiserum to a tartrate-resistant and vanadate-sensitive acid phosphatase (nucleotide tri- and diphosphatase) prepared from rat bone was used in immunocytochemical studies. The antigen was localized in sections of fixed, decalcified tissue (head from rat) using the peroxidase-antiperoxidase bridge (PAP) or the avidin-biotin-peroxidase complex (ABC) technique. Both techniques resulted in similar and specific immunostaining in the following cells and tissues: osteoclasts situated in resorption lacunae, epithelium overlying enamel-free areas of tips of cusps of unerupted molars, cilia of respiratory epithelium, and tissue macrophages. This distribution corresponds to the cellular sites of tartrate-resistant acid phosphatase activity, as revealed by enzyme histochemistry. With the ABC method, staining in osteoclasts was obtained with antiserum dilutions of up to 1:10,000. Biochemical studies revealed that vanadate-sensitive acid ATPase activity in liver subcellular fractions was almost exclusively confined to lysosomes. Thus, the immunostaining has revealed the presence of the tartrate-resistant and vanadate-sensitive nucleotide phosphatase in many cells associated with tissue resorption and phagocytosis.
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000818:Animals; D001081:Apyrase / Q000378:metabolism*; D001842:Bone and Bones / Q000201:enzymology; D011232:Chemical Precipitation; D004351:Drug Resistance; D005260:Female; D006651:Histocytochemistry; D007120:Immunochemistry; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains; D013644:Tartrates / Q000494:pharmacology*; D014070:Tooth / Q000201:enzymology / Q000254:growth & development; D014638:Vanadates; D014639:Vanadium / Q000494:pharmacology*
D013644:Tartrates; D014639:Vanadium; D014638:Vanadates; D010744:Phosphoric Monoester Hydrolases; D000251:Adenosine Triphosphatases; D001081:Apyrase; C029768:tartaric acid