title: Effect of complement depletion by cobra venom factor on fowlpox virus infection in chickens and chicken embryos.
abstract: The course of infection with an attenuated strain of fowlpox virus (FPV), which is known to induce antibody-independent activation of complement via the alternative pathway, was investigated in 1- to 3-day-old chickens and 14-day-old chicken embryos by treatment with cobra venom factor (CVF). CVF was found to inhibit complement activity transiently via the alternative pathway but not via the classical pathway. In chickens treated with CVF, virus growth in the skin was enhanced, and pock lesions tended to disseminate, leading to fatal infection in some birds. Histologically, an acute inflammation at an early stage of infection (within 3 days) was inhibited, and virus content in the pock lesion was increased. In chicken embryos with immature immune capacities, CVF treatment caused changes in pock morphology from clear pocks to diffuse ones, an increase in virus content in the pock, and inhibition of cell infiltration. Thus, FPV infection was aggravated in both CVF-treated chickens and chicken embryos. These results are discussed in relation to roles of complement in the elimination of virus at an early stage of FPV infection.
mesh_terms: D000818:Animals; D002642:Chick Embryo; D002645:Chickens / Q000382:microbiology; D003167:Complement Activation / Q000187:drug effects; D003165:Complement System Proteins / Q000502:physiology*; D004546:Elapid Venoms / Q000494:pharmacology*; D005586:Fowlpox / Q000276:immunology*; D005587:Fowlpox virus / Q000254:growth & development / Q000276:immunology*; D008213:Lymphocyte Activation / Q000187:drug effects; D011212:Poxviridae / Q000276:immunology*
chemical_list: D004546:Elapid Venoms; C016310:cobra venom factor; D003165:Complement System Proteins