title: Hepatic retinol metabolism. Distribution of retinoids, enzymes, and binding proteins in isolated rat liver cells.
abstract: The main retinoids and some binding proteins and enzymes involved in retinol metabolism have been quantified in different types of rat liver cells. Hepatic perisinusoidal stellate cells contained 28-34 nmol of retinoids/10(6) cells, and parenchymal liver cells contained 0.5-0.8 nmol of retinoids/10(6) cells, suggesting that as much as 80% of more of total liver retinoids might be stored in stellate cells with the rest stored in parenchymal cells. Isolated endothelial cells and Kupffer cells contained very low levels of retinoids. More than 98% of the retinoids recovered in stellate cells were retinyl esters. Isolated parenchymal and stellate cell preparations both contained considerable retinyl palmitate hydrolase and acyl-CoA:retinol acyltransferase activities. Parenchymal cells accounted for about 75-80% of the total hepatic content of these two enzyme activities, with the rest located in stellate cells. On a cell protein basis, the concentrations of both of these activities were much greater in stellate cells than in parenchymal cells. In contrast, cholesteryl oleate and triolein hydrolase activities were fairly evenly distributed in all types of liver cells. Large amounts of cellular retinol binding proteins were also found in parenchymal and stellate cells. Although parenchymal cells accounted for more than 90% of hepatic cellular retinol binding protein, the concentration of the protein in stellate cells (per unit protein) was 22 X greater than that in parenchymal cells. Stellate cells were also enriched in cellular retinoic acid binding protein. Thus, both parenchymal and stellate cells contain substantial amounts of retinoids and of the enzymes and intracellular binding proteins involved in retinol metabolism. Stellate cells are particularly enriched in these several components.
mesh_terms: D000217:Acyltransferases / Q000032:analysis*; D000818:Animals; D002265:Carboxylic Ester Hydrolases / Q000032:analysis*; D002352:Carrier Proteins / Q000032:analysis*; D066298:In Vitro Techniques; D008099:Liver / Q000032:analysis* / Q000378:metabolism; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D018168:Receptors, Retinoic Acid; D012176:Retinoids / Q000032:analysis*; D051142:Retinol O-Fatty-Acyltransferase; D012177:Retinol-Binding Proteins / Q000032:analysis*; D054840:Retinol-Binding Proteins, Cellular; D014801:Vitamin A / Q000378:metabolism
chemical_list: D002352:Carrier Proteins; D018168:Receptors, Retinoic Acid; D012176:Retinoids; D012177:Retinol-Binding Proteins; D054840:Retinol-Binding Proteins, Cellular; C022521:retinol-palmitate esterase; D014801:Vitamin A; D000217:Acyltransferases; D051142:Retinol O-Fatty-Acyltransferase; D002265:Carboxylic Ester Hydrolases