Reduction of lactogenic receptors in female hamster liver due to the human growth hormone analog produced by plerocercoids of the tapeworm, Spirometra mansonoides.
The inductive effect of GH on hepatic lactogenic receptors is suspected of being due to a direct somatogenic action. Plerocercoid larvae of the tapeworm, Spriometra mansonoides, produce a factor that stimulates body growth, suppresses endogenous GH, and specifically displaces [125I]human (h) GH from hepatic receptors. Plerocercoid growth factor (PGF) mimics the growth-promoting actions of GH, but it has not been shown to duplicate all of the activities reported for GH. An important function of GH is its role in the maintenance of liver receptors for lactogenic hormones. This study was undertaken to determine if treatment of female hamsters with PGF would increase, decrease, or have no effect on liver receptors that bind hGH. Since hGH binds to somatogenic as well as lactogenic receptors, it was necessary to demonstrate the specificity of PGF's effects on [125I]hGH binding. PGF-treated (15 pleocercoids sc) hamsters had accelerated body growth, suppressed serum GH, and a marked reduction in [125I]hGH and [125I]ovine PRL binding to hepatic microsomes. Specific binding of [125I] bGH was unaltered by PGF treatment. The difference in [125I] hGH binding was due to a reduction in receptor number and not to receptor occupancy or reduced affinity. Serum GH was normalized after 10 days of estradiol benzoate (25 micrograms/day) injections, but the binding capacity for [125I]hGH of the PGF-treated group was less than half that of the control group. The fact that estrogen injections normalized serum GH, but not hGH binding, indicates that down-regulation of these receptors by PGF cannot be entirely explained on the basis of reduced levels of serum GH. The lack of any effect of PGF treatment on [125I]bGH binding suggests that the hepatic somatogenic receptors were not involved and that the reduction in receptors for [125I]hGH was associated with the lactogenic component of hGH.
D000818:Animals; D001835:Body Weight; D002462:Cell Membrane / Q000378:metabolism; D002589:Cestoda / Q000502:physiology*; D006224:Cricetinae; D004169:Diphyllobothriasis / Q000503:physiopathology*; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D013006:Growth Hormone / Q000097:blood / Q000378:metabolism*; D006133:Growth Substances / Q000502:physiology*; D006168:Guinea Pigs; D036341:Intercellular Signaling Peptides and Proteins; D008099:Liver / Q000033:anatomy & histology; D008647:Mesocricetus; D009929:Organ Size; D010903:Pituitary Gland, Anterior / Q000033:anatomy & histology; D011388:Prolactin / Q000378:metabolism; D011869:Radioligand Assay; D011956:Receptors, Cell Surface / Q000378:metabolism*; D011981:Receptors, Prolactin; D011986:Receptors, Somatotropin; D013146:Spirometra / Q000502:physiology*
D006133:Growth Substances; D036341:Intercellular Signaling Peptides and Proteins; D011956:Receptors, Cell Surface; D011981:Receptors, Prolactin; D011986:Receptors, Somatotropin; C043871:plerocercoid growth factor; D004958:Estradiol; D011388:Prolactin; D013006:Growth Hormone