Partial purification and characterization of cytochrome P-450 from human placenta.
Two isozymes of cytochrome P-450 were partially purified to specific contents of 7.0 and 0.5 nmol/mg of protein, respectively, from placenta of non-smoking women by chromatography on octyl Sepharose, hydroxylapatite, DEAE-cellulose and CM-cellulose. NADPH-cytochrome P-450 reductase was purified from phenobarbital-induced mouse liver and from human placenta and was combined with cytochrome P-450 and dilauroylphosphatidylcholine to reconstitute the cytochrome P-450 monooxygenase system. Substrates investigated were benzo[a]pyrene, 7-ethoxycoumarin and delta 4-androstene-3,17-dione.
D000735:Androstenedione / Q000378:metabolism; D000818:Animals; D001580:Benzopyrenes / Q000378:metabolism; D002850:Chromatography, Gel / Q000379:methods; D003374:Coumarins / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000302:isolation & purification*; D005260:Female; D006801:Humans; D051379:Mice; D008861:Microsomes / Q000201:enzymology; D009251:NADPH-Ferrihemoprotein Reductase / Q000302:isolation & purification; D017137:Nonoxynol; D010920:Placenta / Q000201:enzymology*; D011092:Polyethylene Glycols / Q000494:pharmacology; D011247:Pregnancy
D001580:Benzopyrenes; D003374:Coumarins; D017137:Nonoxynol; C017299:7-ethoxycoumarin; D011092:Polyethylene Glycols; D000735:Androstenedione; C026487:benzo(e)pyrene; D003577:Cytochrome P-450 Enzyme System; D009251:NADPH-Ferrihemoprotein Reductase