[Endonucleolysis of chromatin in thymocytes of irradiated and non-irradiated rats].
It was shown that in conditions optimal for Ca/Mg endonuclease, chromatin endonucleolysis in the nuclei and thymocytes occurs due to internucleosome fragmentation of DNA. Irradiation activates chromatin degradation in thymocytes washed by a buffer containing 0.25 M sucrose, 10 mM tris-HCl, pH 7.2, 3 mM MgCl2, and does not influence this process in thymocytes washed by 10 mM tris-HCl, pH 7.2, 3 mM MgCl2.
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism / Q000528:radiation effects; D002843:Chromatin / Q000378:metabolism* / Q000528:radiation effects; D004247:DNA / Q000378:metabolism / Q000528:radiation effects*; D004587:Electrophoresis, Agar Gel; D004720:Endonucleases / Q000378:metabolism*; D008297:Male; D011089:Polydeoxyribonucleotides / Q000378:metabolism / Q000528:radiation effects; D051381:Rats; D013601:T-Lymphocytes / Q000378:metabolism / Q000528:radiation effects*; D014916:Whole-Body Irradiation
D002843:Chromatin; D011089:Polydeoxyribonucleotides; D004247:DNA; D004720:Endonucleases
Endonukleoliz khromatina v timotsitakh obluchennykh i neobluchennykh krys.