title: Promoter selectivity of E. coli RNA polymerase: analysis of the promoter system of convergently-transcribed dnaQ-rnh genes.
abstract: Promoter properties were analyzed for the convergently-overlapped E. coli genes coding for the DNA polymerase III epsilon subunit (dnaQ) and the ribonuclease H (rnh). The rates of open complex formation for a single promoter of the rnh gene and two tandem promoters of the dnaQ gene were constant whether they are located on a single DNA fragment or separated into individual fragments. The relative expression levels of these three promoters, as measured using an in vitro mixed transcription system, varied differentially depending on the concentration of RNA polymerase. At low enzyme concentrations, the downstream promoter (P2) of the dnaQ gene was utilized preferentially, but the upstream promoter (P1) was utilized as well when the enzyme concentration was increased. This indicates different physiological roles between the two dnaQ promoters. The level of rnh transcription was as low as that of dnaQ-1 RNA synthesis but the rnh promoter was utilized as well as the dnaQ P2 promoter when it was separated from the dnaQ promoters. This implies a promoter interference between the convergently transcribed genes.
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D004258:DNA Polymerase III / Q000235:genetics*; D004262:DNA Restriction Enzymes; D004259:DNA-Directed DNA Polymerase / Q000235:genetics*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D005796:Genes; D005798:Genes, Bacterial; D007700:Kinetics; D011401:Promoter Regions, Genetic; D012260:Ribonucleases / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D012321:DNA-Directed RNA Polymerases; D004258:DNA Polymerase III; D004259:DNA-Directed DNA Polymerase; D012260:Ribonucleases; D004262:DNA Restriction Enzymes; C029600:ribonuclease B