Glucocorticoid responsiveness of the transcriptional enhancer of Moloney murine sarcoma virus.
The long terminal repeat of Moloney Murine Sarcoma Virus (MoMSV) contains an imperfect direct repeat that serves as a strong transcriptional enhancer. The strength of the MoMSV enhancer is strongly dependent on the presence of glucocorticoid hormone. Mapping studies in combination with DNAase I footprinting experiments define the presence of glucocorticoid regulatory elements at the promoter-proximal ends of each enhancer repeat. These elements behave like inducible enhancers: their regulatory activity is independent of position and orientation when they are linked in cis to a heterologous promoter. These data demonstrate the modular nature of the MoMSV enhancer and identify the glucocorticoid receptor as one of the trans-acting factors that interact with the MoMSV enhancer and mediate its transcriptional activity.
D000818:Animals; D003907:Dexamethasone / Q000378:metabolism / Q000494:pharmacology*; D004742:Enhancer Elements, Genetic / Q000187:drug effects*; D005786:Gene Expression Regulation / Q000187:drug effects; D005809:Genes, Regulator / Q000187:drug effects*; D008980:Moloney murine sarcoma virus / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D011965:Receptors, Glucocorticoid / Q000378:metabolism; D012091:Repetitive Sequences, Nucleic Acid / Q000187:drug effects; D009053:Sarcoma Viruses, Murine / Q000235:genetics*; D018139:Simplexvirus / Q000235:genetics; D013937:Thymidine Kinase; D014158:Transcription, Genetic / Q000187:drug effects*
D011965:Receptors, Glucocorticoid; D003907:Dexamethasone; D013937:Thymidine Kinase