T lymphocyte cloning from rejected human kidney allografts. Growth frequency and functional/phenotypic analysis.
Mechanically harvested lymphocytes invading an irreversibly rejected human kidney allograft were seeded at limiting dilution to calculate the frequency of growing precursors. Optimal growth frequency (1/13) was obtained when Epstein-Barr virus (EBV)-transformed donor B lymphocytes were used as stimulators (D-BLCL) in the presence of interleukin 2 (IL-2). The 55 clones analyzed were all T11+ and T3+, and all expressed DR antigens (45% were T8+ and 55% T4+). Only one clone had a double-labeled (T4+ T8+) surface. All cells proliferated significantly against D-BLCL, although T4+ clones had a significantly shorter average doubling time than T8+ clones. Nearly all T8+ clones were specifically cytotoxic for D-BLCL, while both T4 and T8 did not react against K562, autologous EBV-BLCL, and third-party EBV-BLCL. Detectable IL-2 was found in the culture supernatants of only a minority of clones (all T4+).
D000911:Antibodies, Monoclonal / Q000032:analysis; D002455:Cell Division; D002470:Cell Survival; D002472:Cell Transformation, Viral; D002999:Clone Cells / Q000166:cytology; D005260:Female; D006084:Graft Rejection; D004854:Herpesvirus 4, Human; D006801:Humans; D007376:Interleukin-2 / Q000096:biosynthesis; D007668:Kidney / Q000276:immunology; D016030:Kidney Transplantation; D008875:Middle Aged; D010641:Phenotype; D013601:T-Lymphocytes / Q000166:cytology*
D000911:Antibodies, Monoclonal; D007376:Interleukin-2