title: Inhibition of growth of Rhizobium japonicum by cyclic GMP.
abstract: Exogenous cyclic guanosine-3',5'-monophosphate (cGMP) inhibited the growth of Rhizobium japonicum at less than 100 microM. Other nucleotides, including cyclic AMP, cyclic IMP, and cyclic CMP, had no inhibitory effect even at higher concentrations nor was the inhibition by cGMP reversed by cyclic AMP. The inhibitory effect was independent of the carbon and nitrogen source(s) used. cGMP did not inhibit the growth of any other species of bacterium tested, including several fast-growing Rhizobium species. The kinetics of growth inhibition are multiphasic, with no apparent effect for several hours after addition, followed by a period of total inhibition. Subsequently, growth resumed at a slower rate. Resumption of growth was not due to destruction of the nucleotide. Studies of the intracellular cGMP concentration did not reveal significant changes in cells grown under aerobic or microaerobic conditions. No effect of cGMP on the derepression of respiratory nitrate reductase was observed.
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis; D000242:Cyclic AMP / Q000494:pharmacology; D003563:Cyclic CMP / Q000494:pharmacology; D006152:Cyclic GMP / Q000032:analysis / Q000494:pharmacology*; D007289:Cyclic IMP / Q000494:pharmacology; D004269:DNA, Bacterial / Q000096:biosynthesis; D009565:Nitrate Reductases / Q000378:metabolism; D012231:Rhizobium / Q000032:analysis / Q000187:drug effects* / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism; D013045:Species Specificity
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D007289:Cyclic IMP; D003563:Cyclic CMP; D000242:Cyclic AMP; D009565:Nitrate Reductases; D006152:Cyclic GMP