title: Nucleotide sequence of the entire protein coding region of canine distemper virus polymerase-associated (P) protein mRNA.
abstract: The entire coding region of the polymerase-associated (P) protein gene of canine distemper virus has been sequenced. A single cDNA clone which represents 98% of the mRNA encoding this protein was used to determine the nucleotide sequence. The sequence predicts a major protein of 507 amino acids and a molecular weight of 54 936. There is also a second, overlapping, open reading frame with a start signal 21 bases downstream of the first AUG which could code for a protein of 174 amino acids with a predicted molecular weight of 20 292. This arrangement of the genome for the P protein of canine distemper virus is exactly analogous to that published recently for the P gene of measles virus (Bellini, W.J. et al., 1985, J. Virol. 53, 908-919). When the sequences are aligned at the first AUG, considerable homology is seen at both the nucleotide and protein sequence level.
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D003062:Codon; D004262:DNA Restriction Enzymes; D004217:Distemper Virus, Canine / Q000201:enzymology / Q000235:genetics*; D005796:Genes; D005814:Genes, Viral; D008970:Molecular Weight; D009363:Neoplasm Proteins / Q000235:genetics*; D010957:Plasmids; D012333:RNA, Messenger / Q000235:genetics*; D035165:Transcription Factors, General; D035602:Transcriptional Elongation Factors
chemical_list: D003062:Codon; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D035165:Transcription Factors, General; D035602:Transcriptional Elongation Factors; C018648:transcription factor S-II; D004262:DNA Restriction Enzymes