An improved preparation of phosphatidylglycerophosphate.
Phosphatidylglycerophosphate is not normally accumulated in the reaction mixture. It was found that octyl-beta-D-glucopyranoside greatly stimulated the synthesis and inhibited the degradation of phosphatidylglycerophosphate. The phenomenon could be used for the preparation of phosphatidylglycerophosphate from CDPdiacylglycerol and the sn-glycero-3-phosphate with the crude membrane enzyme preparation. The optimal concentration of octyl-beta-D-glucopyranoside to be used for such a purpose was 24.5 mM.
D055598:Chemical Phenomena; D002621:Chemistry; D004926:Escherichia coli / Q000187:drug effects / Q000378:metabolism; D005960:Glucosides / Q000494:pharmacology; D010715:Phosphatidylglycerols / Q000302:isolation & purification*; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism; D010770:Phosphotransferases / Q000378:metabolism; D017855:Transferases (Other Substituted Phosphate Groups)
D005960:Glucosides; D010715:Phosphatidylglycerols; C034358:phosphatidylglycerophosphate; C018619:octyl-beta-D-glucoside; D010770:Phosphotransferases; D017855:Transferases (Other Substituted Phosphate Groups); C020676:CDP-diacylglycerol-glycerol-3-phosphate 3-phosphatidyltransferase; D010744:Phosphoric Monoester Hydrolases; C026991:phosphatidylglycerophosphatase