A novel RNA from early region 3 of adenovirus-2.
A novel RNA species was detected by Northern blotting among the polyadenylated nuclear transcripts from early region 3 of adenovirus-2. Preliminary mapping showed that it encompasses the E3 leader 1 and part of IVS 1. Primer extension experiments revealed that its 5' end (C) was the same as that of all E3 transcripts, and S1 nuclease mapping showed that its 3' end was a polyadenylation site (P1) known to be used in the late phase of infection. The body of this RNA is 618-619 nucleotides long and we propose to name it E3-CP1. Kinetic studies suggest that it is a primary transcript as are the E3 premessenger RNAs.
D000260:Adenoviruses, Human / Q000235:genetics*; D001483:Base Sequence; D004720:Endonucleases; D006367:HeLa Cells / Q000166:cytology; D006801:Humans; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000032:analysis* / Q000235:genetics; D012260:Ribonucleases; D015719:Single-Strand Specific DNA and RNA Endonucleases; D014158:Transcription, Genetic
D012367:RNA, Viral; D004720:Endonucleases; D012260:Ribonucleases; D015719:Single-Strand Specific DNA and RNA Endonucleases