Wheat germ phosphoglycerate mutase: purification, polymorphism, and inhibition.
An improved method for purifying the bisphosphoglycerate-independent phosphoglycerate mutase from wheat germ has been devised. The method yields enzyme with a specific activity of 2,300 units/mg in 0.1 M Tris-C1 at pH 8.7 and 30 degrees C. Electrophoresis on electrofocusing and analytical polyacrylamide gels reveals only one protein band (pI = 7.3); however, under denaturing conditions (sodium dodecyl sulfate polyacrylamide gel electrophoresis), two prominent enzyme forms, with molecular masses of 63 and 74 kDa, manifest themselves along with several minor, high molecular mass components (126-141 kDa). Non-denaturing exclusion chromatography shows that both major species are catalytically active, and suggests that each species is capable of participating in reversible monomer/dimer association. Wheat germ mutase is inhibited by time-dependent reactions involving either polydentate chelators or sulfhydryl reagents.
D001731:Bisphosphoglycerate Mutase / Q000037:antagonists & inhibitors / Q000302:isolation & purification* / Q000378:metabolism; D002614:Chelating Agents / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D007525:Isoelectric Focusing; D007700:Kinetics; D046911:Macromolecular Substances; D008970:Molecular Weight; D010770:Phosphotransferases / Q000302:isolation & purification*; D010944:Plants / Q000201:enzymology*; D011110:Polymorphism, Genetic; D013439:Sulfhydryl Reagents / Q000494:pharmacology; D014908:Triticum
D002614:Chelating Agents; D046911:Macromolecular Substances; D013439:Sulfhydryl Reagents; D010770:Phosphotransferases; D001731:Bisphosphoglycerate Mutase