Cloning of a streptomycin-production gene directing synthesis of N-methyl-L-glucosamine.
A Streptomyces bikiniensis DNA fragment complementing a deficiency in streptomycin (Sm) production was cloned on the plasmid vector pIJ385. Host strain S. bikiniensis SD 1 used as the recipient in cloning displayed deficiency in biosynthesis of N-methyl-L-glucosamine, one of the moieties of Sm. The cloned fragment on the multicopy plasmid pIJ385 conferred sevenfold increase in Sm production in comparison with the wild-type parental strain. By subcloning, the region complementing the Sm deficiency of SD 1 was narrowed to a 3.0-kb fragment.
D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D005798:Genes, Bacterial; D008536:Meglumine / Q000378:metabolism*; D010957:Plasmids; D013012:Sorbitol / Q000031:analogs & derivatives*; D013302:Streptomyces / Q000235:genetics*; D013307:Streptomycin / Q000235:genetics*
D013012:Sorbitol; D008536:Meglumine; D004262:DNA Restriction Enzymes; D013307:Streptomycin