Nucleotide sequence of the gene determining plasmid-mediated citrate utilization.
The citrate utilization determinant from transposon Tn3411 has been cloned and sequenced, and its polypeptide products have been characterized in minicell experiments. The nucleotide sequence was determined for a 2,047-base-pair BglII restriction endonuclease fragment that includes the citrate determinant. This region contains an open reading frame that would encode a 431-amino-acid very hydrophobic polypeptide and which is preceded by a reasonable ribosomal binding site. However, the single polypeptide found in minicell experiments had an apparent molecular weight of 35,000 on sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
D000595:Amino Acid Sequence; D001483:Base Sequence; D002872:Chromosome Deletion; D002951:Citrates / Q000378:metabolism*; D019343:Citric Acid; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics; D005470:Fluorometry; D009690:Nucleic Acid Conformation; D010641:Phenotype; D010957:Plasmids
D002951:Citrates; D004251:DNA Transposable Elements; D019343:Citric Acid; D004262:DNA Restriction Enzymes