Changes in membrane permeability in cells infected by human cytomegalovirus.
To analyze whether human cytomegalovirus could induce changes in membrane permeability we studied both Hygromycin B (HyB) inhibition of protein synthesis and glycine and choline uptake during viral replication. A higher sensitivity to HyB was observed both immediately after virus adsorption and in concomitance with the release of viral progeny. HyB sensitivity immediately after virus adsorption was not associated with changes in membrane permeability to glycine and choline while 60 hours p.i. a remarkable increase in choline uptake was detected.
D001692:Biological Transport / Q000187:drug effects; D002463:Cell Membrane Permeability / Q000187:drug effects; D002472:Cell Transformation, Viral; D002794:Choline / Q000378:metabolism; D003587:Cytomegalovirus / Q000235:genetics*; D004261:DNA Replication / Q000187:drug effects; D004622:Embryo, Mammalian; D005260:Female; D005347:Fibroblasts / Q000187:drug effects; D005998:Glycine / Q000378:metabolism; D006801:Humans; D006921:Hygromycin B / Q000494:pharmacology; D007202:Indicators and Reagents; D007700:Kinetics; D009861:Onium Compounds / Q000378:metabolism; D009943:Organophosphorus Compounds / Q000378:metabolism; D011247:Pregnancy; D014176:Protein Biosynthesis; D014779:Virus Replication / Q000187:drug effects
D007202:Indicators and Reagents; D009861:Onium Compounds; D009943:Organophosphorus Compounds; D006921:Hygromycin B; D002794:Choline; D005998:Glycine; C013289:tetraphenylphosphonium