[Serologic diagnosis of primary cytomegalovirus infections in patients following kidney transplantation using the indirect immunofluorescence technic and the complement-fixation test].
Sera of 22 recipients of kidney transplants with clinically and serologically (complement binding reaction) ascertained cytomegalovirus infection were retrospectively examined for virus-specific antibodies of the class IgM by means of the indirect immunofluorescence test. Already two to five days after the appearance of the adequate clinical symptoms (leucopenia, fever, T-lymphocytopenia, increase of creatinine and thrombocytopenia) CMV-IgM-antibodies could be proved. On average a positive complement binding reaction was found only two weeks later. In other 13 patients of the actual control programme the CMV-IgM-antibody findings first obtained were compared with the results of the complement fixing reaction obtained later. Neither falsely positive nor falsely negative results could be found.
D000914:Antibodies, Viral / Q000032:analysis*; D000936:Antigen-Antibody Complex / Q000032:analysis; D003168:Complement Fixation Tests; D003587:Cytomegalovirus / Q000276:immunology; D003586:Cytomegalovirus Infections / Q000175:diagnosis / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D006801:Humans; D016030:Kidney Transplantation; D011183:Postoperative Complications / Q000175:diagnosis
D000914:Antibodies, Viral; D000936:Antigen-Antibody Complex
Serologische Diagnostik primärer Zytomegalievirusinfektionen bei Patienten nach Nierentransplantation mittels indirekter Immunfluoreszenztechnik und Komplementbindungsreaktion.