Action of cobra venom cardiotoxin on chick embryonal fibroblasts transformed with a temperature-sensitive mutant of Rous sarcoma virus.
The cytolytic action of cardiotoxin analogue III from the venom of the Formosan cobra on chick embryonal fibroblasts transformed with a temperature-sensitive mutant of Rous sarcoma virus was investigated. The 50% effective dose of the toxin for the cells cultured at a non-permissive temperature (41 degrees C) or for noninfected normal cells was about 8 micrograms/ml whereas the value was 2 micrograms/ml for the cells cultured at a permissive temperature (36 degrees C). This indicates that the transformed cells became more susceptible to the cytolytic action of the toxin than the non-transformed cells.
D000818:Animals; D001358:Avian Sarcoma Viruses / Q000235:genetics*; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D002478:Cells, Cultured; D002642:Chick Embryo; D004179:Cobra Cardiotoxin Proteins / Q000633:toxicity*; D004546:Elapid Venoms / Q000302:isolation & purification / Q000633:toxicity*; D005347:Fibroblasts / Q000187:drug effects / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D009154:Mutation; D013696:Temperature
D004179:Cobra Cardiotoxin Proteins; D004546:Elapid Venoms