The separation of sheep and human serum "A"-esterase activity into the lipoprotein fraction by ultracentrifugation.
Using sheep and human serum the relationship between centrifugation time and yield of total lipoprotein, HDL-cholesterol and "A"-esterase in lipoprotein was studied employing different centrifuge rotors. More rapid separation of these components was obtained with a vertical rotor than with an angled rotor. The procedures commonly employed for lipoprotein separation gave low yields of lipoprotein "A"-esterase and HDL-cholesterol. The separation of sheep serum "A"-esterase into the lipoprotein fraction was not in phase with that of HDL-cholesterol and the pattern of separation was different from that in human serum. These results provide further evidence that serum "A"-esterase activity is associated with different species of HDL-particle.
D000818:Animals; D043303:Aryldialkylphosphatase; D008076:Cholesterol, HDL / Q000097:blood; D006801:Humans; D008074:Lipoproteins / Q000097:blood* / Q000302:isolation & purification; D010744:Phosphoric Monoester Hydrolases / Q000097:blood* / Q000302:isolation & purification; D012756:Sheep; D013045:Species Specificity; D014461:Ultracentrifugation
D008076:Cholesterol, HDL; D008074:Lipoproteins; D010744:Phosphoric Monoester Hydrolases; D043303:Aryldialkylphosphatase