Human prostatic acid phosphatase has phosphotyrosyl protein phosphatase activity.
The major secreted isoenzyme of human prostatic acid phosphatase (PAcP) (EC 3.1.3.2), which catalyses p-nitrophenyl phosphate (PNPP) hydrolysis at acid pH values, was found to have phosphotyrosyl protein phosphatase activity since it dephosphorylated three different phosphotyrosine-containing protein substrates. Several lines of evidence are presented to show that the phosphotyrosyl phosphatase and PAcP are the same enzyme. A highly purified PAcP enzyme preparation which contains a single N-terminal peptide sequence was used to test for the phosphotyrosyl phosphatase activity. Both activities comigrated during gel filtration by high performance liquid chromatography. Phosphotyrosyl phosphatase activity and PNPP acid phosphatase activity exhibited similar sensitivities to different effectors. Both phosphatase activities showed the same thermal stability. Specific anti-PAcP antibody reacted to the same extent with both phosphatase activities. PNPP acid phosphatase activity was competitively inhibited by the phosphotyrosyl phosphatase substrate. To characterize further the phosphotyrosyl phosphatase activity, the Km values using different phosphoprotein substrates were determined. The apparent Km values for phosphorylated angiotensin II, anti-pp60src immunoglobulin G and casein were in the nM range for phosphotyrosine residues, which was about 50-fold lower than the Km for phosphoserine residues in casein.
D000135:Acid Phosphatase / Q000037:antagonists & inhibitors / Q000276:immunology / Q000378:metabolism*; D000804:Angiotensin II / Q000378:metabolism; D000906:Antibodies / Q000276:immunology; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism*; D007700:Kinetics; D010749:Phosphoprotein Phosphatases / Q000378:metabolism*; D017027:Protein Tyrosine Phosphatases; D013379:Substrate Specificity; D013696:Temperature
D000906:Antibodies; D007527:Isoenzymes; D000804:Angiotensin II; D010749:Phosphoprotein Phosphatases; D000135:Acid Phosphatase; D017027:Protein Tyrosine Phosphatases