Epstein-Barr virus-specific DNA polymerase in virus-nonproducer Raji cells.
Virus-nonproducer Raji cells, when induced to early antigen synthesis by 12-O-tetradecanoyl-phorbol-13-acetate and sodium butyrate, showed an increase in DNA polymerase activity. This enzyme has the characteristics of a typical Epstein-Barr virus DNA polymerase with regard to chromatographical pattern and biological properties: it is eluted from DEAE-cellulose at 0.08 M NaCl, has a high salt resistance, is sensitive to phosphonoacetic acid and phosphonoformate, and shows a substrate preference for poly(dC)-oligo(dG12-18). The resistance of Epstein-Barr virus polymerase activity to aphidicolin is a property distinct from that of HSV DNA polymerase. Viral DNA polymerase activity increases in the absence of Epstein-Barr virus DNA replication, indicating that this enzyme is an early viral protein.
D000956:Antigens, Viral; D016590:Aphidicolin; D002087:Butyrates / Q000494:pharmacology; D020148:Butyric Acid; D002460:Cell Line; D004261:DNA Replication; D004259:DNA-Directed DNA Polymerase / Q000096:biosynthesis* / Q000378:metabolism; D004224:Diterpenes / Q000494:pharmacology; D004790:Enzyme Induction; D017245:Foscarnet; D004854:Herpesvirus 4, Human / Q000201:enzymology* / Q000276:immunology / Q000502:physiology; D006801:Humans; D010746:Phosphonoacetic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D013379:Substrate Specificity; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014779:Virus Replication
D000956:Antigens, Viral; D002087:Butyrates; D004224:Diterpenes; C033305:Epstein-Barr virus early antigen; D020148:Butyric Acid; D017245:Foscarnet; D016590:Aphidicolin; D004259:DNA-Directed DNA Polymerase; D010746:Phosphonoacetic Acid; D013755:Tetradecanoylphorbol Acetate