Different metabolism by mammalian and horseradish peroxidases in vitro of steroidal estrogens and their catechol metabolites.
Radioactively labeled estradiol-17 beta and 17 alpha-ethynylestradiol and their 2-hydroxy derivatives were incubated with either horseradish peroxidase or mouse uterus peroxidase, and the formation of polar products, which could not be extracted with diethyl ether, and of ether-extractable metabolites was studied. Moreover, the extent of DNA binding was determined. The different peroxidases gave rise to different products, indicating that different pathways in the metabolism of these steroidal estrogens are catalysed by the two peroxidases.
D000818:Animals; D002384:Catalysis; D004247:DNA / Q000378:metabolism; D004967:Estrogens / Q000378:metabolism*; D002393:Estrogens, Catechol / Q000378:metabolism*; D005260:Female; D006735:Horseradish Peroxidase / Q000378:metabolism*; D066298:In Vitro Techniques; D007527:Isoenzymes / Q000378:metabolism*; D051379:Mice; D009195:Peroxidase; D010544:Peroxidases / Q000378:metabolism*; D013045:Species Specificity; D014599:Uterus / Q000201:enzymology
D004967:Estrogens; D002393:Estrogens, Catechol; D007527:Isoenzymes; D004247:DNA; D006735:Horseradish Peroxidase; D010544:Peroxidases; D009195:Peroxidase