title: N6-functionalized congeners of adenosine with high potency at A2-adenosine receptors: potential ligands for affinity chromatography.
abstract: Adenosine analogues substituted at N6 with spacer arms designed for attachment to soluble macromolecules or to solid supports for affinity chromatography are agonists at the A2-adenosine receptor that mediates coronary vasodilation in the dog. The most active analogues had spacer arms terminating in -NH2, -NHCH3 or in a biotin residue. Comparisons of coronary vasoactivity with affinity for brain A1 adenosine receptors identified one biotin-containing analogue as relatively selective for coronary A2 receptors. The complex of this analogue with avidin retained coronary vasoactivity.
mesh_terms: D000241:Adenosine / Q000031:analogs & derivatives* / Q000378:metabolism; D000818:Animals; D001360:Avidin / Q000378:metabolism; D001921:Brain / Q000378:metabolism; D002846:Chromatography, Affinity; D003326:Coronary Circulation / Q000187:drug effects; D004285:Dogs; D011956:Receptors, Cell Surface / Q000378:metabolism*; D011983:Receptors, Purinergic; D012995:Solubility; D014664:Vasodilation / Q000187:drug effects
chemical_list: D011956:Receptors, Cell Surface; D011983:Receptors, Purinergic; D001360:Avidin; D000241:Adenosine