Isolation and serial propagation of turkey rotaviruses in a fetal rhesus monkey kidney (MA104) cell line.
Turkey rotaviruses from the intestinal contents of poults were isolated and serially propagated in MA104 cell monolayers by a simple procedure. The initial virus isolation was done by low-speed centrifugation of the inoculum onto the monolayers, and subsequent passages were accomplished in roller-tube monolayers using trypsin-treated virus suspensions. Each of the turkey rotavirus isolates possessed the morphologic, antigenic, and genomic attributes characteristic of turkey group A rotaviruses. Attempts to isolate and serially propagate turkey rotavirus-like viruses in MA104 cell monolayers by this procedure were unsuccessful. Turkey reoviruses also did not serially propagate in MA104 cell monolayers by this procedure.
D000818:Animals; D002460:Cell Line; D007422:Intestines / Q000382:microbiology*; D007668:Kidney; D008253:Macaca mulatta; D012401:Rotavirus / Q000302:isolation & purification*; D014422:Turkeys / Q000382:microbiology*; D014776:Virus Cultivation