The stereochemical course of phosphoryl transfer catalyzed by herpes simplex virus type I-induced thymidine kinase.
Herpes simplex virus type I (HSV-I)-induced thymidine kinase has been shown to catalyze phosphoryl transfer from adenosine 5'-[gamma-(S)-16O,17O,18O]triphosphate to thymidine with inversion of configuration at phosphorus. The simplest interpretation of this result is that phosphoryl transfer occurs by a single in-line group transfer between ATP and thymidine within the ternary enzyme complex.
D000255:Adenosine Triphosphate / Q000031:analogs & derivatives / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008968:Molecular Conformation; D018139:Simplexvirus / Q000201:enzymology; D013379:Substrate Specificity; D013873:Thionucleotides / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism; D013937:Thymidine Kinase / Q000378:metabolism*; D014764:Viral Proteins / Q000378:metabolism*
D013873:Thionucleotides; D014764:Viral Proteins; C022571:adenosine 5'-O-(3-thiotriphosphate); D000255:Adenosine Triphosphate; D013937:Thymidine Kinase; D013936:Thymidine