Integration of hepatitis B virus DNA in chromosome-specific satellite sequences.
We previously reported the cloning and detailed analysis of the integrated hepatitis B virus sequences in a human hepatoma cell line. We report here the integration of at least one of hepatitis B virus at human satellite DNA sequences. The majority of the cellular sequences identified by this satellite DNA were organized as a multimeric composition of a 0.6-kilobase EcoRI fragment. This clone hybridized in situ almost exclusively to the centromeric heterochromatin of chromosomes 1 and 16 and to a lower extent to chromosome 2 and to the heterochromatic region of the Y chromosome. The immediate flanking host sequence appeared as a hierarchy of repeating units which were almost identical to a previously reported human satellite III DNA sequence.
D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000382:microbiology*; D002460:Cell Line; D002877:Chromosomes, Human; D002900:Chromosomes, Human, 1-3; D002902:Chromosomes, Human, 16-18; D004276:DNA, Satellite / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics*; D006515:Hepatitis B virus / Q000235:genetics*; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000382:microbiology; D009693:Nucleic Acid Hybridization; D011995:Recombination, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D014998:Y Chromosome
D004276:DNA, Satellite; D004279:DNA, Viral