Removal of haem from lipids extracted from intact erythrocytes with particular reference to polyphosphoinositides.
With the use of a modified acid version of a current lipid-extraction technique [Bligh & Dyer (1959) Can. J. Biochem. Physiol. 37, 911-917], 92% of phosphatidylinositol 4,5-bisphosphate obtained by means of three sequential extractions from intact human erythrocytes was obtained during the first one. Some 95% of the haem co-extracted with the lipids could then be removed, with a maximal loss of polyphosphoinositides of less than 3%. About 58 nmol of phosphatidylinositol 4,5-bisphosphate was found per ml of erythrocytes.
D004912:Erythrocytes / Q000032:analysis*; D006418:Heme / Q000032:analysis; D006801:Humans; D008563:Membrane Lipids / Q000097:blood / Q000302:isolation & purification*; D010710:Phosphates / Q000032:analysis; D010716:Phosphatidylinositols / Q000097:blood / Q000302:isolation & purification*
D008563:Membrane Lipids; D010710:Phosphates; D010716:Phosphatidylinositols; D006418:Heme