title: Different complement and granulocyte activation in patients dialyzed with PMMA dialyzers.
abstract: Plasma C3a and C5a levels as well as plasma levels of granulocyte lactoferrin, granulocyte myeloperoxidase and granulocyte elastase in complex with alpha 1-proteinase inhibitor (E-alpha 1PI) were investigated in 10 patients (52.7 +/- 5.9 years) undergoing maintenance hemodialysis (39.4 +/- 12.4 months) with hollow fiber dialyzers made from polymethylmethacrylate. Plasma levels of lactoferrin increased from 166.5 +/- 28.5 to 712.5 +/- 165.9 ng/ml, myeloperoxidase from 59.0 +/- 15.3 to 210.5 +/- 33.9 ng/ml and E-alpha 1PI from 114.2 +/- 18.1 to 681.8 +/- 102.6 ng/ml during dialysis. In contrast, plasma C3a levels rose from 179.8 +/- 33.6 to maximal 276.2 +/- 45.4 ng/ml and C5a from 55.7 +/- 8.1 to maximal 101.1 +/- 14.8 ng/ml. Our data indicate that degranulation of granulocytes occurs during dialysis despite only little complement activation and mild initial granulocytopenia.
mesh_terms: D001672:Biocompatible Materials; D001798:Blood Proteins; D003167:Complement Activation; D003176:Complement C3 / Q000032:analysis*; D015926:Complement C3a; D003182:Complement C5 / Q000032:analysis*; D015936:Complement C5a; D005260:Female; D006098:Granulocytes / Q000201:enzymology*; D006801:Humans; D007688:Kidneys, Artificial; D007781:Lactoferrin / Q000097:blood*; D007782:Lactoglobulins / Q000097:blood*; D008297:Male; D008567:Membranes, Artificial; D008768:Methylmethacrylates; D008875:Middle Aged; D010196:Pancreatic Elastase / Q000097:blood; D009195:Peroxidase / Q000097:blood*; D011480:Protease Inhibitors / Q000097:blood; D006435:Renal Dialysis; D014511:Uremia / Q000201:enzymology / Q000276:immunology / Q000628:therapy; D000515:alpha 1-Antitrypsin
chemical_list: D001672:Biocompatible Materials; D001798:Blood Proteins; D003176:Complement C3; D003182:Complement C5; D007782:Lactoglobulins; D008567:Membranes, Artificial; D008768:Methylmethacrylates; D011480:Protease Inhibitors; D000515:alpha 1-Antitrypsin; D015926:Complement C3a; D015936:Complement C5a; D009195:Peroxidase; D007781:Lactoferrin; D010196:Pancreatic Elastase