A simultaneous quantitation of leukotriene B4 and its omega-oxidized products by gas chromatography-mass spectrometry.
We developed a highly sensitive and specific method for the simultaneous quantitation of leukotriene B4 (LTB4) and its omega-oxidized metabolites (20-hydroxy-LTB4 and 20-carboxy-LTB4) by mass fragmentography using deuterated compounds as internal standards. The ions produced by the cleavage of the C12-13 bond of the methyl ester dimethylisopropylsilyl ether derivatives of LTB4 and its metabolites were measured by selective ion monitorings. The detection limit of LTB4 was less than 10 pg and about 100-fold lower than that by high performance liquid chromatography. By using this method, the synthesis and further metabolism of LTB4 in human polymorphonuclear leukocytes were investigated.
D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D007975:Leukotriene B4 / Q000032:analysis*; D009504:Neutrophils / Q000378:metabolism*; D010084:Oxidation-Reduction
D007975:Leukotriene B4