Influence of bromodeoxyuridine substitution of thymidine on sister-chromatid exchanges and chromosomal aberrations induced by restriction endonucleases.
Different levels of replacement of thymidine by 5-bromodeoxyuridine in mammalian DNA have been used to analyze restriction endonuclease-dependent induction of sister-chromatid exchanges and chromosomal aberrations. Data regarding enzyme action in whole cells and in isolated nuclei are presented and discussed. The results indicate a lack of correlation between enzyme effectiveness and the degree of 5-bromodeoxyuridine substitution in the target sequences, specific to the tested restriction endonucleases.
D000818:Animals; D001973:Bromodeoxyuridine / Q000494:pharmacology*; D002460:Cell Line; D002869:Chromosome Aberrations; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000032:analysis; D004262:DNA Restriction Enzymes / Q000494:pharmacology*; D004357:Drug Synergism; D005260:Female; D005347:Fibroblasts / Q000187:drug effects*; D010053:Ovary; D012854:Sister Chromatid Exchange / Q000187:drug effects*; D013936:Thymidine / Q000494:pharmacology
D004247:DNA; D004262:DNA Restriction Enzymes; D001973:Bromodeoxyuridine; D013936:Thymidine