title: Cellular mechanisms in in vivo production of gamma interferon induced by lipopolysaccharide in mice infected with Mycobacterium bovis BCG.
abstract: Infection of mice with Mycobacterium bovis BCG sensitizes them for immune induction of interferon (IFN)-gamma by specific antigen. These mice were found also to respond to lipopolysaccharide (LPS) and concanavalin A (Con A) with high level production of IFN-gamma in the circulation, which was not observed in control mice. In this study, we compared the IFN-gamma response to LPS with that to Con A in an attempt to clarify the cellular mechanisms responsible for in vivo LPS-induced IFN-gamma production. Consequently, (i) the responses to LPS and Con A differed in the kinetics, that to LPS having a longer lag period. (ii) Spleen cells taken from infected mice produced little IFN-gamma in response to LPS, but they showed a higher IFN-gamma response to Con A than those from control mice. (iii) By treating infected mice with immunosuppressive drugs or antibodies to T and natural killer cells before challenge with the inducers, it was indicated that different cellular systems are involved in the IFN-gamma responses to LPS and to Con A.
mesh_terms: D000818:Animals; D000906:Antibodies; D003208:Concanavalin A / Q000494:pharmacology; D005260:Female; D007371:Interferon-gamma / Q000096:biosynthesis* / Q000494:pharmacology; D007694:Killer Cells, Natural / Q000276:immunology; D007700:Kinetics; D007739:L Cells / Q000276:immunology; D008070:Lipopolysaccharides / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009163:Mycobacterium bovis / Q000276:immunology*; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D014721:Vesicular stomatitis Indiana virus / Q000187:drug effects
chemical_list: D000906:Antibodies; D008070:Lipopolysaccharides; D003208:Concanavalin A; D007371:Interferon-gamma