title: A comparative study of the purine- and pyrimidine-metabolising enzymes of a range of trypanosomatids.
abstract: A range of trypanosomatids (amastigotes and cultured promastigotes of Leishmania mexicana mexicana, cultured promastigotes of L. m. amazonensis, L. donovani and L. tarentolae, culture forms of Crithidia fasciculata, Herpetomonas muscarum muscarum and H. m. ingenoplastis and procyclic trypomastigotes of Trypanosoma brucei brucei) have been surveyed for the presence of purine- and pyrimidine-metabolising enzymes. Several common features were observed, including the presence of nucleosidases, catabolic phosphorylases, phosphoribosyltransferases, kinases and cytidine deaminase and the apparent absence of AMP deaminase, anabolic purine phosphorylase and cytosine deaminase. Significant differences between species were discovered, notably in adenine and adenosine metabolism. Nucleoside phosphotransferase active on inosine was detected in insect trypanosomatids but not in L. m. mexicana.
mesh_terms: D000619:Aminohydrolases / Q000378:metabolism; D000818:Animals; D003421:Crithidia / Q000201:enzymology; D007891:Leishmania / Q000201:enzymology*; D009699:N-Glycosyl Hydrolases / Q000378:metabolism; D010430:Pentosyltransferases / Q000378:metabolism; D010770:Phosphotransferases / Q000378:metabolism; D011714:Pyrans / Q000378:metabolism*; D011743:Pyrimidines / Q000378:metabolism*; D011753:Pyrones / Q000378:metabolism*; D013045:Species Specificity; D014346:Trypanosoma brucei brucei / Q000201:enzymology*; D014351:Trypanosomatina / Q000201:enzymology*
chemical_list: D011714:Pyrans; D011743:Pyrimidines; D011753:Pyrones; D010430:Pentosyltransferases; D010770:Phosphotransferases; D009699:N-Glycosyl Hydrolases; D000619:Aminohydrolases