title: Multiple extractions of hormone receptor from rabbit uteri and human breast tumors.
abstract: The cytosol hormone receptors were extracted from rabbit uteri and human tumors with tris buffer systems for three consecutive homogenization and centrifugation processes to obtain three cytosols C-1, C-2, and C-3. The assay results evaluated revealed that minimal to tremendous amounts of receptor activities were present in C-2 and/or C-3 fractions. The essential experimental conditions and some speculative mechanisms have been discussed in detail.
mesh_terms: D000818:Animals; D001943:Breast Neoplasms / Q000032:analysis*; D003600:Cytosol / Q000032:analysis; D005260:Female; D006801:Humans; D008722:Methods; D011817:Rabbits; D011956:Receptors, Cell Surface / Q000032:analysis*; D011960:Receptors, Estrogen / Q000032:analysis; D011980:Receptors, Progesterone / Q000032:analysis; D014599:Uterus / Q000032:analysis*
chemical_list: D011956:Receptors, Cell Surface; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone