Immunomodulating activity in supernatants from EBV immortalized lymphocytes.
Our earlier work has demonstrated that EBV immortalized B lymphocytes are involved in a factor dependent autostimulatory cycle. Soluble growth stimulating activity was released into culture supernatants by these growing B cells. Growth enhancing (GE) media from B lymphocyte lines, immortalized by EBV infection, contained soluble factor(s) which modulated the Con A response of normal human mononuclear cells. Conditioned media from these lines affected the Con A response in a biphasic manner, stimulating the blastogenic response at lower concentrations, while inhibiting at higher concentrations. At stimulatory concentrations, the blastogenic response to Con A began earlier than in controls and was markedly enhanced by day 2. GE media reduced the initial response of purified B cells to pokeweed mitogen. GE media did not support growth of IL-2 dependent cells. GE media from some EBV-carrying B cell lines had measurable IL-1 activity in the mouse thymocyte PHA response. GE media from LPS stimulated B lymphocyte lines produced significant IL-1-like effects on stimulated mouse thymocytes. These results suggested that these B cell lines may produce IL-1-like factors that cooperate in T cell responses. The possibility that such factors may play a role in B lymphocyte transformation by EBV is discussed.
D000276:Adjuvants, Immunologic / Q000502:physiology*; D001402:B-Lymphocytes / Q000276:immunology*; D002460:Cell Line; D002469:Cell Separation; D002472:Cell Transformation, Viral; D002474:Cell-Free System; D003208:Concanavalin A / Q000494:pharmacology; D003470:Culture Media; D006133:Growth Substances / Q000502:physiology; D004854:Herpesvirus 4, Human / Q000276:immunology*; D006801:Humans; D015847:Interleukin-4; D008213:Lymphocyte Activation; D008222:Lymphokines / Q000502:physiology; D011043:Pokeweed Mitogens / Q000494:pharmacology
D000276:Adjuvants, Immunologic; D003470:Culture Media; D006133:Growth Substances; D008222:Lymphokines; D011043:Pokeweed Mitogens; D003208:Concanavalin A; D015847:Interleukin-4