An evaluation of some in vivo immunization strategies for the production of monoclonal antibodies to insulin and ACTH.
Immunization schedules for the production of monoclonal antibodies to bovine insulin and porcine adreno-corticotrophic hormone (ACTH) have been investigated. Specifically, prime dose, prime route, pre-fusion boost dose and immune status have been evaluated for their effect on both the number of hybrids observed after fusion, and the proportion of wells containing antibody which bound to the immunogen. Although a single optimum protocol was not identified, the results indicate that spleen cells from mice primed at multiple sites should be used for fusion after the peak of the primary antibody response. Excessive hyperimmunization should be avoided. Dose regimes combining a low prime amount and a high pre-fusion boost amount or a high prime amount and a low pre-fusion boost amount (except in the presence of circulating antibody) were favoured. Monitoring of the immune response of animals used in fusion experiments is of paramount importance.
D000324:Adrenocorticotropic Hormone / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal; D002417:Cattle; D002459:Cell Fusion; D005260:Female; D007114:Immunization; D007117:Immunization, Secondary; D007328:Insulin / Q000276:immunology*; D008722:Methods; D051379:Mice; D008807:Mice, Inbred BALB C; D013552:Swine
D000911:Antibodies, Monoclonal; D007328:Insulin; D000324:Adrenocorticotropic Hormone