Construction of genomic libraries of mycobacterial origin: identification of recombinants encoding mycobacterial-specific proteins.
A complete genomic library from Mycobacterium vaccae (2785 recombinants) and a partial genomic library of M. leprae and BCG (300 and 1750 clones, respectively) were constructed in the plasmid pBR322. Bam HI was selected as the restriction endonuclease for obtaining DNA cleavage products. Evidence was obtained for limited expression of the cloned mycobacterial DNA inserts in Escherichia coli. A recombinant has been identified which codes for antigen immunoreactive with rabbit anti-M. leprae antibody but not with anti-H37Rv antibody.
D000942:Antigens, Bacterial / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics* / Q000276:immunology; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D004274:DNA, Recombinant; D005798:Genes, Bacterial; D009161:Mycobacterium / Q000235:genetics; D009163:Mycobacterium bovis / Q000235:genetics* / Q000276:immunology; D009166:Mycobacterium leprae / Q000235:genetics* / Q000276:immunology; D010957:Plasmids
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004274:DNA, Recombinant; D004262:DNA Restriction Enzymes