Calmodulin-like protein from Bacillus subtilis.
The first example of a calmodulin-like activity in a Gram-positive bacterium, Bacillus subtilis, is reported. A calcium ion-dependent, 3', 5' cyclic-AMP phosphodiesterase-stimulating activity was found in the soluble fraction of cell-free extracts of cells sporulating in a chemically-defined medium; activation was reversed by trifluoperazine. The activity was heat stable, bound to phenothiazine-agarose in a calcium ion-dependent manner and was eluted therefrom with buffer containing EGTA, and displaced authentic beef brain calmodulin from its antibody in a radioimmunoassay.
D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000378:metabolism; D000818:Animals; D001412:Bacillus subtilis / Q000032:analysis*; D002118:Calcium / Q000378:metabolism; D002147:Calmodulin / Q000302:isolation & purification*; D002417:Cattle; D009206:Myocardium / Q000201:enzymology; D011863:Radioimmunoassay; D014268:Trifluoperazine / Q000494:pharmacology
D002147:Calmodulin; D014268:Trifluoperazine; D015105:3',5'-Cyclic-AMP Phosphodiesterases; D002118:Calcium