Stereospecific antibodies to propranolol.
The beta-adrenergic antagonist propranolol was activated through its side chain, coupled to bovine serum albumin, and injected into BALB/c mice. After fusion of the splenocytes from these immunized mice with the NS-1 myeloma cell line, two hybridomas, producing monoclonal anti-propranolol antibodies, were isolated. Clone P-49 was monospecific for propranolol, with a significant preference for the 1-stereoisomer, as compared to the d form. On the other hand, clone P-28 cross-reacted with alprenolol as well as some other beta-antagonists. Both classes of antibodies competed with A431 epidermoid carcinoma beta 2-adrenoceptors for the binding of [3H]propranolol. When ascites cells from clone P-28 were fixed with glutaraldehyde, the anti-propranolol monoclonal antibody became cell bound. These cell-bound P-28 antibodies bind propranolol and other beta-adrenergic ligands with a similar ranking order to the soluble monoclonal antibody. The cell-bound antibody displayed a 5-fold higher affinity towards 1-propranolol than the soluble monoclonal antibody. The practical implications of these findings are discussed.
D000911:Antibodies, Monoclonal; D001667:Binding, Competitive; D002294:Carcinoma, Squamous Cell; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007700:Kinetics; D011433:Propranolol / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D011863:Radioimmunoassay; D011943:Receptors, Adrenergic, beta / Q000378:metabolism; D012710:Serum Albumin, Bovine
D000911:Antibodies, Monoclonal; D011943:Receptors, Adrenergic, beta; D012710:Serum Albumin, Bovine; D011433:Propranolol