Identification and characterization of the protein encoded by the human N-myc oncogene.
The human N-myc gene is related to the c-myc proto-oncogene, and has been shown to have transforming potential in vitro. Many studies have reported amplification of N-myc in human neuroblastoma and retinoblastoma cell lines. In primary tumors, amplification of the gene was found to correlate directly with behavior of the tumor. Specific restriction fragments of a partial complementary DNA clone of N-myc from LA-N-5 human neuroblastoma cells were placed into a bacterial expression vector for the purpose of producing antigens representative of the N-myc protein. Rabbits immunized with these antigens produced antisera that recognized a protein of 62-64 kilodaltons in neuroblastoma cells. By several criteria, this protein appears to be part of the same proto-oncogene family as the c-myc protein. Moreover, the antisera to fragments of this protein were capable of histochemically identifying malignant cells in clinical specimens.
D000818:Animals; D001483:Base Sequence; D018288:Carcinoma, Small Cell / Q000378:metabolism; D007106:Immune Sera / Q000276:immunology; D007124:Immunoenzyme Techniques; D015470:Leukemia, Myeloid, Acute / Q000378:metabolism; D008175:Lung Neoplasms / Q000378:metabolism; D009363:Neoplasm Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D009447:Neuroblastoma / Q000378:metabolism; D009857:Oncogenes; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D016271:Proto-Oncogene Proteins c-myc; D011519:Proto-Oncogenes; D011817:Rabbits / Q000276:immunology
D007106:Immune Sera; C000715470:MAS1 protein, human; D009363:Neoplasm Proteins; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins; D016271:Proto-Oncogene Proteins c-myc