Diversity of human pancreatic cancer cell proteinases: role of cell membrane metalloproteinases in collagenolysis and cytolysis.
In this study we have examined the tissue-destructive proteinases of human pancreatic ductal cancer cell lines derived initially from xenogenic transplants. Cancer cell organelles were isolated following nitrogen cavitation using sucrose density gradient centrifugation. Serine, cysteine, and metalloproteinases were assayed using radiolabeled protein and synthetic substrates. Tumor-induced RBC lysis was quantitated by measuring the release of isotope from 59Fe-labeled RBCs co-cultivated with tumor cells or subcellular fractions. Enzyme inhibitors with specificity toward different classes of proteinases were used in the above assays to categorize the enzymes responsible for substrate degradation. Results indicated that intact pancreatic cancer cells (RWP-1 and RWP-2 cell lines), cell homogenate, and cytosol contain proteinases which were able to degrade [3H]collagen (type I) and [3H]gelatin and lyse normal RBCs. Cancer cell membrane fractions were enriched in collagenolytic, gelatinolytic, and cytolytic activities which could be abrogated by EDTA but not by inhibitors of serine or cysteine proteinases, which indicates that metalloproteinases are the active enzymes in these assays. Although plasminogen activator and cysteine proteinases were also enriched in the tumor cell membranes, these activities were not required for collagen degradation or cytolysis. We conclude that human cancer cell membrane proteinases are advantageously situated to facilitate damage to surrounding normal tissues.
D002460:Cell Line; D002462:Cell Membrane / Q000201:enzymology; D003546:Cysteine Endopeptidases; D010450:Endopeptidases / Q000378:metabolism*; D005780:Gelatin / Q000378:metabolism; D006461:Hemolysis; D006801:Humans; D008666:Metalloendopeptidases; D003012:Microbial Collagenase / Q000378:metabolism*; D008854:Microscopy, Electron; D009362:Neoplasm Metastasis; D010190:Pancreatic Neoplasms / Q000201:enzymology* / Q000473:pathology; D012697:Serine Endopeptidases; D013347:Subcellular Fractions / Q000201:enzymology; D013997:Time Factors
D005780:Gelatin; D010450:Endopeptidases; D012697:Serine Endopeptidases; D003546:Cysteine Endopeptidases; D008666:Metalloendopeptidases; D003012:Microbial Collagenase