Antigenic analysis of yellow fever virus glycoproteins: use of monoclonal antibodies in enzyme-linked immunosorbent assays.
A sensitive enzyme-linked immunosorbent assay with biotin and streptavidin (B/SA ELISA) was developed for the specific detection of yellow fever (YF) viruses. Monoclonal antibodies (MCA) specific for the envelope (E) glycoprotein or the non-structural glycoprotein (NV3) of YF virus-infected cell lysates were tested by antigen and antibody capture B/SA ELISA against YF viruses and a large number of other flaviviruses. In an antigen capture assay, with suckling mouse brain antigens, MCA directed against the envelope glycoprotein clearly differentiated YF viruses from any other flaviviruses, wild-type YF viruses from YF vaccine viruses and YF17D-204 substrains from other YF vaccine viruses. Specific MCA could identify YF viruses in an antibody capture assay with concentrated YF-infected tissue culture supernatant medium as the solid phase. On the basis of the principles established above, MCA binding profiles of purified YF virus strains were compared. No qualitative differences in immunoreactivity could be detected between closely related strains of YF virus. An antibody capture assay on infected tissue culture monolayers was developed to enable in situ detection of YF viruses using MCA specific for both the envelope glycoprotein and the non-structural NV3 glycoprotein.
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral / Q000032:analysis*; D001426:Bacterial Proteins; D001710:Biotin; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D005416:Flavivirus / Q000276:immunology / Q000302:isolation & purification; D006023:Glycoproteins / Q000032:analysis*; D019809:Streptavidin; D015005:Yellow fever virus / Q000276:immunology / Q000302:isolation & purification*
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D001426:Bacterial Proteins; D006023:Glycoproteins; D001710:Biotin; D019809:Streptavidin