title: Cloning simulation in the cage environment.
abstract: The CAGE/GEM(TM) software toolkit for genetic engineering is briefly described. The system functionally uses color graphics and is menu driven. It integrates genetics and features information ("Overlays") with information based on sequence analysis ("Representations"). The system is structured around CAD (Computer Aided Design) principles. The CAGE (Computer Aided Genetic Engineering) aspects of the software are emphasized and illustrated by a simulated cloning of the hepatitis B core antigen gene into the BAMHI site of plasmid pBR322.
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D003201:Computers; D004262:DNA Restriction Enzymes / Q000378:metabolism; D015245:Deoxyribonuclease BamHI; D005818:Genetic Engineering; D006512:Hepatitis B Core Antigens / Q000235:genetics*; D007256:Information Systems; D010957:Plasmids; D012984:Software
chemical_list: D006512:Hepatitis B Core Antigens; D004262:DNA Restriction Enzymes; D015245:Deoxyribonuclease BamHI