title: 1731, a new retrotransposon with hormone modulated expression.
abstract: We report here the characterisation of 1731, a new copia-like element of Drosophila melanogaster. 1731 was first isolated in a screening for ecdysterone modulated genes. This element is about 4.6 Kb long and is flanked by two long terminal repeats (LTRs) 336 base pairs in length. The whole 1731 element is transcribed into polyA+ RNAs, and these transcripts decrease rapidly upon hormonal treatment. 1731 is moderately repeated in the fly genome and slightly amplified in Kc/cells where extrachromosomal circular forms are found. The LTRs were sequenced in one cloned copy of 1731 and show a structural organisation similar to that of several other copia-like elements and retroviral proviruses. Small nucleotide stretches, similar to those found in Mouse Mammary Tumor Virus LTRs and known to be important in its regulation by a steroid hormone, occur in 1731 LTRs.
mesh_terms: D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004251:DNA Transposable Elements; D004331:Drosophila melanogaster / Q000235:genetics*; D004441:Ecdysterone / Q000502:physiology*; D005796:Genes; D009693:Nucleic Acid Hybridization; D010957:Plasmids
chemical_list: D004251:DNA Transposable Elements; D004441:Ecdysterone; D004262:DNA Restriction Enzymes