Structure of viral DNA in a rat cell line transformed by the cloned EcoRI-C fragment of adenovirus 12.
A DNA segment carrying viral DNA was cloned from a rat cell line transformed by the cloned EcoRI-C fragment (0 to 16.4 map units) of human adenovirus type 12(Ad12), and the viral sequence in the clone was analysed. The cloned segment contained the region from nucleotide positions 118 to 3520 of the Ad12 genome in the middle. No unique structure was found at the viral and non-viral DNA junctions. When examined the transforming activity, the conserved viral sequence was able to transform rat 3Y1 cells efficiently. Southern blotting analysis of the viral sequence in five re-transformed cell lines showed that the viral sequence was inserted at different sites of cellular DNA. These results indicate that (I) the Ad12 DNA moiety from the enhancer-promoter region of the E1A gene to the end of the E1B gene contains enough information for efficient transformation of the rat cell, and (II) integration of the viral sequence at unique cellular sites is not prerequisite for transformation.
D000260:Adenoviruses, Human / Q000235:genetics*; D000818:Animals; D010582:Bacteriophage lambda / Q000235:genetics; D001483:Base Sequence; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000302:isolation & purification*; D015246:Deoxyribonuclease EcoRI; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D051381:Rats; D011991:Receptors, Virus / Q000378:metabolism
D004279:DNA, Viral; D011991:Receptors, Virus; D004262:DNA Restriction Enzymes; D015246:Deoxyribonuclease EcoRI