Identification of NGF receptor in chromatin of melanoma cells using monoclonal antibody to cell surface NGF receptor.
A 230 KDa species of Nerve Growth Factor (NGF) receptor was immunoprecipitated from EcoRI-digested chromatin of melanoma cells using a monoclonal antibody to the 75 KDa cell surface NGF receptor. The chromatin NGF receptor was shown to exist tightly bound to DNase II-sensitive sequences which, upon growth factor binding, became resistant to DNase II digestion.
D000911:Antibodies, Monoclonal / Q000276:immunology*; D002460:Cell Line; D002843:Chromatin / Q000032:analysis*; D004706:Endodeoxyribonucleases / Q000494:pharmacology; D006801:Humans; D008545:Melanoma / Q000032:analysis*; D011956:Receptors, Cell Surface / Q000032:analysis* / Q000276:immunology; D017475:Receptors, Nerve Growth Factor
D000911:Antibodies, Monoclonal; D002843:Chromatin; D011956:Receptors, Cell Surface; D017475:Receptors, Nerve Growth Factor; D004706:Endodeoxyribonucleases; C019329:deoxyribonuclease II