Efficient reversion of simian sarcoma virus-transformation and inhibition of growth factor-induced mitogenesis by suramin.
Simian sarcoma virus, an acutely transforming primate retrovirus with capacity to induce gliomas and sarcomas in experimental animals, has acquired its transforming properties by transducing the cellular gene sequences that encode one of the constituent chains of platelet-derived growth factor. Suramin, a drug used in the treatment of trypanosomiasis and onchocerciasis, has previously been reported to inhibit the interaction of platelet-derived growth factor with its cell surface receptor. We show here that suramin efficiently reverts the simian sarcoma virus-induced transformed phenotype in human and rat fibroblasts and propose that this is due to neutralization of an externalized v-sis product. Moreover, we show that suramin inhibits the action of a broad spectrum of growth factors.
D000818:Animals; D001358:Avian Sarcoma Viruses; D002118:Calcium / Q000494:pharmacology; D002453:Cell Cycle / Q000187:drug effects; D002472:Cell Transformation, Viral / Q000187:drug effects*; D004815:Epidermal Growth Factor / Q000037:antagonists & inhibitors*; D066246:ErbB Receptors; D006801:Humans; D051379:Mice; D010982:Platelet-Derived Growth Factor / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000378:metabolism; D017479:Receptors, Platelet-Derived Growth Factor; D012842:Sarcoma Virus, Woolly Monkey; D009053:Sarcoma Viruses, Murine; D013498:Suramin / Q000494:pharmacology*
D010982:Platelet-Derived Growth Factor; D011956:Receptors, Cell Surface; D013498:Suramin; D004815:Epidermal Growth Factor; D066246:ErbB Receptors; D017479:Receptors, Platelet-Derived Growth Factor; D002118:Calcium