The cytochrome c oxidase test for the rapid detection of psychrotrophic bacteria in milk.
A new, rapid, simple and cheap method for the detection of the predominant psychrotrophic bacteria in raw and pasteurized milks is described, using tetramethyl-p-phenylene-diamine dihydrochloride to detect cytochrome c oxidase which in general is not present in the non-psychrotrophic bacterial milk flora. The test is sensitive to samples containing over 10(4) organisms/ml. Correlation coefficients of 0.92 and 0.84 between dye oxidation and viable counts for pasteurized and raw milk samples, respectively, were found.
D000818:Animals; D001407:Bacillus / Q000201:enzymology / Q000254:growth & development; D002417:Cattle; D003080:Cold Temperature; D003576:Electron Transport Complex IV / Q000032:analysis*; D004926:Escherichia coli / Q000201:enzymology / Q000254:growth & development; D005516:Food Microbiology; D006090:Gram-Negative Bacteria / Q000201:enzymology / Q000254:growth & development; D013294:Lactococcus lactis / Q000201:enzymology / Q000254:growth & development; D008722:Methods; D008892:Milk / Q000382:microbiology*; D010655:Phenylenediamines / Q000378:metabolism; D011549:Pseudomonas / Q000201:enzymology / Q000254:growth & development; D013291:Streptococcus / Q000201:enzymology / Q000254:growth & development
D010655:Phenylenediamines; D003576:Electron Transport Complex IV