Activated murine alpha-globin gene is not preferentially associated with the nuclear matrix.
The association of the murine alpha-globin gene with the nuclear matrix was studied in three different states of the gene: inactive (EAT cells), potentially active (MEL cells) and active (induced MEL cells). When "native" nuclei were digested with DNase I it was found that the nuclear matrix was not enriched in alpha-globin DNA sequences in all three different types of cells. A nuclease-hypersensitive site in the 5'-flanking region of the alpha-globin gene was detected in the induced MEL-cells.
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism*; D004247:DNA / Q000302:isolation & purification; D004262:DNA Restriction Enzymes; D003850:Deoxyribonuclease I / Q000378:metabolism; D005796:Genes; D005914:Globins / Q000235:genetics*; D051379:Mice; D009693:Nucleic Acid Hybridization
D005914:Globins; D004247:DNA; D004262:DNA Restriction Enzymes; D003850:Deoxyribonuclease I