Simplification of a commercially available serum angiotensin-converting enzyme determination.
The spectrophotometric method for the determination of angiotensin-converting enzyme in serum using p-hydroxyhippuryl-L-histidyl-L-leucine as substrate is commercially available as a test kit. It shows excellent linearity over the whole range of catalytic concentration found in serum. We describe several modifications of this method to simplify and economize the procedure.
D002384:Catalysis; D003124:Colorimetry; D006801:Humans; D007202:Indicators and Reagents; D009842:Oligopeptides / Q000097:blood*; D007703:Peptidyl-Dipeptidase A / Q000097:blood*; D011933:Reagent Kits, Diagnostic; D013050:Spectrometry, Fluorescence
D007202:Indicators and Reagents; D009842:Oligopeptides; D011933:Reagent Kits, Diagnostic; C046711:4-hydroxyhippuryl-histidyl-leucine; D007703:Peptidyl-Dipeptidase A