Frequency and directionality of gene conversion events involving the CYC7-H3 mutation in Saccharomyces cerevisiae.
The CYC7-H3 mutation is a 5-kb deletion that causes overproduction of iso-2 cytochrome c. Unlike most mutations in yeast, the CYC7-H3 mutation is preferentially lost when it is involved in a gene conversion event. We have shown that cloned copies of CYC7-H3 DNA that are inserted into the yeast genome are associated with a high frequency of recombination and aberrant segregation events. Since parity in conversion frequency was observed when the extensive insertion/deletion heterozygosity at this locus was eliminated, we conclude that the CYC7-H3 sequences are inherently capable of acting as donors or recipients in gene conversion events, although they are unlikely to act as donors when they are located opposite a large heterology. DNA sequence comparisons revealed similarities between the CYC7-H3 junction region and the 2-micron circle DNA region that is involved in site-specific recombination.
D002872:Chromosome Deletion; D003574:Cytochrome c Group / Q000235:genetics*; D045304:Cytochromes c; D004262:DNA Restriction Enzymes; D004251:DNA Transposable Elements; D005785:Gene Conversion; D005796:Genes; D005800:Genes, Fungal; D009154:Mutation; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D003574:Cytochrome c Group; D004251:DNA Transposable Elements; C032139:iso-2-cytochrome C; D045304:Cytochromes c; D004262:DNA Restriction Enzymes