Immunoperoxidase method with human immune serum globulin for broad-spectrum detection of cultivable human enteric viruses: application to enumeration of cultivable viruses in environmental samples.
The detection and enumeration of most cultivable human enteric viruses from water is possible if samples are first inoculated onto a suitable cell line such as MA-104 or BGM. Virus growth is then detected by an indirect immunoperoxidase method with human immune serum globulin as the source of antibody to most enteric viruses. The number of positive cell cultures in the immunoperoxidase assay is used to calculate the virus titer (as a most probable number) in the sample assayed.
D002460:Cell Line; D004770:Enterovirus / Q000302:isolation & purification* / Q000648:ultrastructure; D005916:Globulins; D006507:Hepatovirus / Q000302:isolation & purification; D006801:Humans; D007106:Immune Sera; D007124:Immunoenzyme Techniques
D005916:Globulins; D007106:Immune Sera