Hepatitis B virus transcripts in a human hepatoma cell line, Hep 3B.
Hep 3B, a human hepatoma cell line was examined for its RNA hybridizable to the hepatitis B virus sequence. Using probes that covered different regions of the hepatitis B virus genome, five species of RNA were observed of sizes 4.0, 3.3, 2.9, 2.6 and 2.2 kilobases. The RNAs covered surface antigen gene, pre-S and X regions. None of them had a core antigen sequence. RNA with a 4.0 kilobase size was the most abundant. Using S1 nuclease analysis, its 5' end of hepatitis B virus sequence was mapped at pre-S region and its 3' end of viral sequence was mapped at DR region.
D000951:Antigens, Neoplasm / Q000235:genetics; D000954:Antigens, Surface / Q000235:genetics; D006528:Carcinoma, Hepatocellular / Q000235:genetics*; D002460:Cell Line; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004273:DNA, Neoplasm / Q000032:analysis; D004279:DNA, Viral / Q000032:analysis; D015245:Deoxyribonuclease BamHI; D015246:Deoxyribonuclease EcoRI; D015247:Deoxyribonuclease HindIII; D004720:Endonucleases / Q000378:metabolism; D006515:Hepatitis B virus / Q000235:genetics*; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D012334:RNA, Neoplasm / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis; D015719:Single-Strand Specific DNA and RNA Endonucleases; D014158:Transcription, Genetic
D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D004273:DNA, Neoplasm; D004279:DNA, Viral; D012334:RNA, Neoplasm; D012367:RNA, Viral; D004720:Endonucleases; D004262:DNA Restriction Enzymes; D015245:Deoxyribonuclease BamHI; D015246:Deoxyribonuclease EcoRI; D015247:Deoxyribonuclease HindIII; D015719:Single-Strand Specific DNA and RNA Endonucleases