Nucleic acid chromatography: substitute solid support material for RPC-5 columns.
Chromatography of tRNA and DNA fragments on columns of reverse-phase 5 (RPC-5) exchange material has been widely employed for analytical and preparative studies. The Plaskon bead that formed the solid support on which a quaternary amine was absorbed is no longer commercially available. A Voltalef bead is available and provides similar, though not identical, chromatograms for Asp-tRNA, Ser-tRNA, and certain DNA fragments. Procedures are described for preparation of the column packing and for long-term operation of the column.
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004247:DNA / Q000302:isolation & purification*; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000302:isolation & purification; D015252:Deoxyribonucleases, Type II Site-Specific; D012343:RNA, Transfer / Q000302:isolation & purification*; D012346:RNA, Transfer, Amino Acyl / Q000302:isolation & purification
D004279:DNA, Viral; D012346:RNA, Transfer, Amino Acyl; D004247:DNA; D012343:RNA, Transfer; D004262:DNA Restriction Enzymes; D015252:Deoxyribonucleases, Type II Site-Specific; C024403:GGCC-specific type II deoxyribonucleases