Effects of recombinant interleukin-2 on resting human T lymphocytes.
The effects of recombinant interleukin-2 on resting T lymphocytes were examined in vitro. T cell enriched populations were isolated from the peripheral venous blood of three normal individuals by Ficoll-Hypaque gradient separation and standard sheep erythrocyte rosetting techniques. Interleukin-2 at concentrations of 100 and 1,000 U/ml stimulated tritiated thymidine incorporation, Tac antigen expression, interferon production, and blast transformation in T cell cultures. These changes were observed in the absence of cell proliferation. The effects of interleukin-2 at a concentration of 100 U/ml were substantially blocked by the addition of a 1:1,000 dilution of anti-Tac antibody to the culture medium indicating that the effects observed were mediated by the Tac receptor. These results indicate that recombinant interleukin-2 can have demonstrable functional and morphologic effects on unstimulated human T cells.
D000328:Adult; D000954:Antigens, Surface / Q000032:analysis; D004247:DNA / Q000096:biosynthesis; D006801:Humans; D066298:In Vitro Techniques; D007371:Interferon-gamma / Q000096:biosynthesis; D007376:Interleukin-2 / Q000276:immunology*; D008213:Lymphocyte Activation; D011971:Receptors, Immunologic / Q000096:biosynthesis; D015375:Receptors, Interleukin-2; D011994:Recombinant Proteins / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7
D000954:Antigens, Surface; D007376:Interleukin-2; D011971:Receptors, Immunologic; D015375:Receptors, Interleukin-2; D011994:Recombinant Proteins; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7; D007371:Interferon-gamma; D004247:DNA