Expression of herpes simplex virus glycoprotein B gene in yeast.
The DNA sequence coding for herpes simplex virus type 1 glycoprotein B was placed under control of the acid phosphatase promoter of the yeast Saccharomyces cerevisiae in a plasmid capable of replicating in both yeast and Escherichia coli. Yeast transformed by the plasmid synthesized immunologically active glycoprotein B polypeptide.
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D005796:Genes; D005800:Genes, Fungal; D006023:Glycoproteins / Q000235:genetics*; D007668:Kidney; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D018139:Simplexvirus / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics*
D004279:DNA, Viral; D006023:Glycoproteins; D014764:Viral Proteins; D004262:DNA Restriction Enzymes