[Steroidogenic action of 2 analogs of ovine LH on cells isolated from ovine corpus luteum].
Lysine residues appear to play an important role in the biological activity of luteinizing hormone or lutropin (LH). Some derivatives obtained by chemical modification such as N-methylated LH exhibit the same hormonal activity than LH in the different steps of the mechanism of steroidogenesis. Some others, on the contrary, preserve the hormonal activity only at some steps. In this work was investigated the action of ovine LH and some derivatives on isolated cells prepared from ovine corpora lutea. Guanidinated LH (which is able to bind to LH receptors in Leydig cells but whose steroidogenic potency is very low) exhibits no binding or steroidogenic activity in the female (sheep or rat). As a consequence guanidyl LH can act as an inhibitor of LH action in the male (Leydig cells).
D000818:Animals; D003338:Corpus Luteum / Q000378:metabolism*; D005260:Female; D006041:Goats; D006146:Guanidines / Q000494:pharmacology; D066298:In Vitro Techniques; D008184:Luteal Cells / Q000378:metabolism*; D007986:Luteinizing Hormone / Q000031:analogs & derivatives* / Q000494:pharmacology; D008297:Male; D008745:Methylation; D010053:Ovary / Q000378:metabolism; D011374:Progesterone / Q000096:biosynthesis*; D051381:Rats; D011956:Receptors, Cell Surface / Q000378:metabolism; D011974:Receptors, LH; D013737:Testis / Q000378:metabolism; D013997:Time Factors
D006146:Guanidines; D011956:Receptors, Cell Surface; D011974:Receptors, LH; D011374:Progesterone; D007986:Luteinizing Hormone
Activité stéroïdogénique de deux analogues de la LH ovine sur cellules isolées de corps jaunes ovins.