title: An endonuclease from Chlamydomonas reinhardtii that cleaves the sequence TATA.
abstract: An endodeoxyribonuclease, designated CreI, was purified 16,000-fold from zygotes of the eukaryote Chlamydomonas reinhardtii. CreI preferentially attacks the sequence TATA producing double strand breaks with 3'-phosphomonoester and 5'-hydroxyl termini. The endonuclease has an Mr = 27,000 and requires Ca2+ at pH 7.5 for optimal activity.
mesh_terms: D001483:Base Sequence; D002118:Calcium / Q000494:pharmacology; D002696:Chlamydomonas / Q000201:enzymology*; D003001:Cloning, Molecular; D004247:DNA; D004262:DNA Restriction Enzymes / Q000302:isolation & purification / Q000378:metabolism*; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D008970:Molecular Weight; D013379:Substrate Specificity
chemical_list: D004247:DNA; D004262:DNA Restriction Enzymes; C048901:endodeoxyribonuclease CreI; D008274:Magnesium; D002118:Calcium