Hemolysis and membrane lipid changes induced by xanthine oxidase in vitamin E deficient red cells.
A procedure to induce hemolysis by the hypoxanthine-xanthine oxidase reaction was developed and applied to vitamin E deficient red blood cells (RBCs) in rats. The reaction system was as follows: 0.16 mM hypoxanthine, 0.05 U/ml xanthine oxidase in 2.5% RBC suspensions with an isotonic buffer (pH 7.4) containing 10 mM phosphate buffer and 125 mM saline (277 mOsm). Hemolysis was observed to depend on the vitamin E concentrations in the RBCs. Hemolysis was inhibited by catalase but not by SOD. After the reaction with vitamin E deficient RBCs, an increase in TBARS in the aqueous phase of the reaction mixture was observed. This accompanied the increase in fluorescent substances in the lipid extracts, in association with a significant decrease in the PE and PS of the RBCs, and a decrease in arachidonic acid in membrane lipids. The above changes were almost completely inhibited by tocopherol incorporated into vitamin E deficient RBCs.
D000818:Animals; D004912:Erythrocytes / Q000187:drug effects* / Q000378:metabolism; D006461:Hemolysis / Q000187:drug effects*; D006861:Hydrogen Peroxide / Q000097:blood; D008297:Male; D008563:Membrane Lipids / Q000097:blood*; D051381:Rats; D011919:Rats, Inbred Strains; D013481:Superoxides / Q000097:blood; D014811:Vitamin E Deficiency / Q000097:blood*; D014969:Xanthine Oxidase / Q000494:pharmacology*
D008563:Membrane Lipids; D013481:Superoxides; D006861:Hydrogen Peroxide; D014969:Xanthine Oxidase