Effect of high and low density lipoproteins on corticotropin-mediated cortisol synthesis by bovine zona fasciculata cells.
The role of bovine HDL and LDL in supporting corticotropin-stimulated steroidogenesis has been investigated, using acutely dispersed zona fasciculata cells. Using a dose of corticotropin sufficient to maximally stimulate steroidogenesis (in the absence of lipoproteins) both HDL and LDL increased steroidogenesis in a dose-dependent manner. At higher concentrations of lipoprotein, HDL caused approx 3-fold greater increase in steroid production than did LDL. Taken with the knowledge that HDL is the major cholesterol carrier in bovine serum, these findings suggest that in cattle HDL is a major source of cholesterol for steroidogenesis.
D000302:Adrenal Cortex / Q000378:metabolism*; D000324:Adrenocorticotropic Hormone / Q000502:physiology*; D000818:Animals; D002417:Cattle; D008076:Cholesterol, HDL / Q000494:pharmacology; D008078:Cholesterol, LDL / Q000494:pharmacology; D006854:Hydrocortisone / Q000096:biosynthesis*; D066298:In Vitro Techniques; D008075:Lipoproteins, HDL / Q000494:pharmacology*; D008077:Lipoproteins, LDL / Q000494:pharmacology*
D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D000324:Adrenocorticotropic Hormone; D006854:Hydrocortisone