No radiosensitivity-related change in plasma membrane properties in X- or gamma-irradiated L5178Y-R and L5178Y-S cells.
Two strains of murine lymphoma cells, L5178Y-R (LY-R) and L5178Y-S (LY-S) differ in radiosensitivity (D0 ca 1 and 0.5, respectively), in Na+/K+ and Mg2+-ATPase activities and susceptibility to heat; their fatty acid composition is also slightly different. Nevertheless, neither Na+-dependent amino acid uptake, nor membrane fluidity change after X- or gamma-irradiation (10 Gy under aerobic conditions, 27 Gy under extreme hypoxia). Although in LY-S cells there is a decrease in partition coefficient in a two-phase system, which indicates a late (24 h after irradiation) change in surface charge, this change is not related to viability, membrane fluidity, amino acid transport and survival.
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D017301:Ca(2+) Mg(2+)-ATPase / Q000378:metabolism; D002462:Cell Membrane / Q000528:radiation effects*; D002784:Cholesterol / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D005720:Gamma Rays; D007940:Leukemia L5178 / Q000532:radiotherapy*; D007942:Leukemia, Experimental / Q000532:radiotherapy*; D008560:Membrane Fluidity / Q000528:radiation effects; D008563:Membrane Lipids / Q000032:analysis; D051379:Mice; D011836:Radiation Tolerance; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism
D000596:Amino Acids; D005227:Fatty Acids; D008563:Membrane Lipids; D002784:Cholesterol; D017301:Ca(2+) Mg(2+)-ATPase; D000254:Sodium-Potassium-Exchanging ATPase