Effect of slate dust on the rat erythrocyte membrane composition: in vitro and in vivo studies.
The biochemical composition of rat erythrocyte ghost membrane obtained by hypotonic lysis and slate-dust-induced lysis were compared in vitro. The phospholipids and glycosamine contents decreased in slate-dust-exposed erythrocyte membrane, whereas there were no changes in protein content. The in vitro and in vivo association of silica, leaching from slate dust, with the components of rat erythrocyte ghost membrane, has also been demonstrated. The interaction of silica with membrane constituents is proposed as a mechanism of action of slate-dust toxicity.
D000606:Amino Sugars / Q000097:blood; D000818:Animals; D002855:Chromatography, Thin Layer; D004391:Dust / Q000009:adverse effects*; D004910:Erythrocyte Membrane / Q000378:metabolism*; D005260:Female; D006461:Hemolysis; D008563:Membrane Lipids / Q000097:blood; D008565:Membrane Proteins / Q000097:blood; D010743:Phospholipids / Q000097:blood; D011485:Protein Binding; D051381:Rats; D012822:Silicon Dioxide / Q000097:blood
D000606:Amino Sugars; D004391:Dust; D008563:Membrane Lipids; D008565:Membrane Proteins; D010743:Phospholipids; D012822:Silicon Dioxide