Determination of DNA sequence changes induced by ethyl methanesulfonate in human cells, using a shuttle vector system.
The DNA sequence changes for 54 mutations induced in human cells by the alkylating agent ethyl methanesulfonate are reported. The mutations were obtained by using a shuttle vector system with the bacterial lacI gene as the target. Of the 54 mutations obtained, 53 were G:C to A:T transitions.
D000260:Adenoviruses, Human / Q000235:genetics; D001483:Base Sequence; D002460:Cell Line; D004247:DNA / Q000235:genetics*; D004249:DNA Damage; D005020:Ethyl Methanesulfonate / Q000633:toxicity*; D005822:Genetic Vectors; D006801:Humans; D007668:Kidney; D009154:Mutation; D010957:Plasmids; D013539:Simian virus 40 / Q000235:genetics
D004247:DNA; D005020:Ethyl Methanesulfonate