The pattern of restriction enzyme-induced banding in the chromosomes of chimpanzee, gorilla, and orangutan and its evolutionary significance.
The pattern of banding induced by five restriction enzymes in the chromosome complement of chimpanzee, gorilla, and orangutan is described and compared with that of humans. The G banding pattern induced by Hae III was the only feature common to the four species. Although hominid species show almost complete chromosomal homology, the restriction enzyme C banding pattern differed among the species studied. Hinf I did not induce banding in chimpanzee chromosomes, and Rsa I did not elicit banding in chimpanzee and orangutan chromosomes. Equivalent amounts of similar satellite DNA fractions located in homologous chromosomes from different species or in nonhomologous chromosomes from the same species showed different banding patterns with identical restriction enzymes. The great variability in frequency of restriction sites observed between homologous chromosome regions may have resulted from the divergence of primordial sequences changing the frequency of restriction sites for each species and for each chromosomal pair. A total of 30 patterns of banding were found informative for analysis of the hominid genealogical tree. Using the principle of maximum parsimony, our data support a branching order in which the chimpanzee is more closely related to the gorilla than to the human.
D000818:Animals; D005075:Biological Evolution; D002871:Chromosome Banding; D002875:Chromosomes / Q000648:ultrastructure; D004262:DNA Restriction Enzymes; D004276:DNA, Satellite / Q000235:genetics; D006071:Gorilla gorilla / Q000235:genetics*; D015186:Hominidae / Q000235:genetics*; D006801:Humans; D007621:Karyotyping; D002679:Pan troglodytes / Q000235:genetics*; D011148:Pongo pygmaeus / Q000235:genetics*; D013045:Species Specificity
D004276:DNA, Satellite; D004262:DNA Restriction Enzymes