title: Structural organization and nucleotide sequence of mouse c-myb oncogene: activation in ABPL tumors is due to viral integration in an intron which results in the deletion of the 5' coding sequences.
abstract: Bacteriophage libraries of mouse DNA were screened for sequences homologous to the v-myb oncogene and two overlapping clones containing the v-myb related region were isolated. Restriction enzyme mapping, heteroduplex analysis and nucleotide sequence analysis revealed the presence of nine exons. Six of these exons are homologous to the v-myb region while the other three exons are derived from the 5' region which is deleted in the viral oncogene. The sequences downstream to the sixth v-myb exon are not included in the 17 kbp of DNA sequences analyzed in this study. Comparison of the structure of the normal c-myb clone with its rearranged couterpart present in plasmacytoid lymphosarcomas revealed that the rearrangements occur in this locus as a result of viral integration. Present studies demonstrate that such a viral insertion interrupts the c-myb coding region at a region identical to that observed in the generation of the v-myb gene of avian myeloblastosis virus and results in the synthesis of mRNAs that lack the same 5' coding region.
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D009189:Avian Myeloblastosis Virus; D001483:Base Sequence; D002874:Chromosome Mapping; D004262:DNA Restriction Enzymes; D004273:DNA, Neoplasm / Q000235:genetics*; D003673:Defective Viruses / Q000235:genetics; D005786:Gene Expression Regulation; D008228:Lymphoma, Non-Hodgkin / Q000235:genetics*; D051379:Mice; D008979:Moloney murine leukemia virus / Q000235:genetics; D011519:Proto-Oncogenes; D011995:Recombination, Genetic
chemical_list: D004273:DNA, Neoplasm; D004262:DNA Restriction Enzymes