Monoclonal antibody detection of 2-acetyl-aminofluorene-modified DNA probes for the specific detection of nucleic acids in hybridization procedures.
We describe the use of acetoxy-acetyl-aminofluorene-modified DNA probes in several hybridization techniques. Hybrids were detected with the help of a monoclonal antibody raised against AAF-guanosine and a second antibody coupled to an enzyme. The sensitivity achieved with AAF-DNA probes routinely detected 0.25 pg DNA bound to a filter. AAF-DNA probes were highly stable and were prepared by simple chemical modification of DNA. Their use as a possible diagnostic tool is discussed.
D015073:2-Acetylaminofluorene / Q000276:immunology; D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D001345:Autoradiography; D003101:Collodion; D004247:DNA / Q000032:analysis* / Q000276:immunology; D004926:Escherichia coli / Q000235:genetics; D006157:Guanosine Monophosphate / Q000276:immunology; D006515:Hepatitis B virus / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D009693:Nucleic Acid Hybridization; D010209:Paper
D000911:Antibodies, Monoclonal; D006157:Guanosine Monophosphate; D003101:Collodion; D004247:DNA; D015073:2-Acetylaminofluorene