A method for purifying the platelet membrane glycoprotein IIb-IIIa complex.
A method has been developed for the rapid isolation of platelet membrane glycoproteins (GP) IIb and IIIa. This method produces an excellent yield and does not require the prior isolation of platelet membranes. Outdated platelets were washed and solubilized in Triton X-100. Concanavalin A affinity chromatography was used to purify a platelet glycoprotein fraction. The concanavalin A-retained glycoproteins were eluted and adsorbed with a heparin-Sepharose column to remove a major contaminant, thrombospondin. Sephacryl S-300 gel filtration was used as the final purification step to remove most fibrinogen and low-molecular-weight contaminants. Wheat germ agglutinin affinity chromatography was used to completely remove trace amounts of fibrinogen. The purified GP IIb and GP IIIa were analyzed by sucrose gradient sedimentation and found to consist of heterodimer complexes.
D001792:Blood Platelets / Q000032:analysis*; D002462:Cell Membrane / Q000032:analysis; D002846:Chromatography, Affinity / Q000379:methods*; D002850:Chromatography, Gel; D003208:Concanavalin A; D005340:Fibrinogen / Q000302:isolation & purification; D006801:Humans; D037102:Lectins; D008970:Molecular Weight; D010980:Platelet Membrane Glycoproteins; D011487:Protein Conformation; D011956:Receptors, Cell Surface / Q000097:blood*; D014909:Wheat Germ Agglutinins
D037102:Lectins; D010980:Platelet Membrane Glycoproteins; D011956:Receptors, Cell Surface; D014909:Wheat Germ Agglutinins; D003208:Concanavalin A; D005340:Fibrinogen