Physical and genetic structure of the IncN plasmid R15.
Restriction sites for seven hexanucleotide-specific endonucleases were located on the map of the conjugative IncN plasmid R15 (SmrSurHgr, 62.3 kb). The distribution of the cleavage sites is strongly asymmetric. Twenty-eight of thirty-four sites for BamHI, EcoRI, HindIII, SalI, SmaI, and PstI were located close to or within the sequences of an IS5-like element and the transposons Tn2353 and Tn2354. By analysis of R15::Tn1756 deletion derivatives and recombinant plasmids harboring R15 fragments, the genetic determinants for the streptomycin, sulfonamide, and mercury resistances were mapped, as well as the regions necessary for EcoRII restriction-modification and for plasmid replication and conjugation. The features of physical and genetic structures of the plasmid R15 and other IncN plasmids are discussed.
D010582:Bacteriophage lambda / Q000235:genetics; D002874:Chromosome Mapping; D003227:Conjugation, Genetic; D004262:DNA Restriction Enzymes; D004251:DNA Transposable Elements; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D011514:Proteus vulgaris / Q000235:genetics; D011815:R Factors; D012093:Replicon
D004251:DNA Transposable Elements; D004262:DNA Restriction Enzymes