title: The nucleotide sequence of the nitrogen regulation gene ntrB and the glnA-ntrBC intergenic region of Klebsiella pneumoniae.
abstract: The nucleotide sequence of the Klebsiella pneumoniae ntrB gene and the glnA-ntrBC intergenic region has been determined. NtrB encodes a 38,409 Dalton polypeptide with a potential DNA-binding domain between residues 67 and 86. This N-terminal domain may play a role in the co-operative control of ntr-regulated promoters by the ntrB and ntrC products. Mapping of in vivo transcripts with S1 nuclease identified three transcripts in the glnA-ntrBC intergenic region. Two transcripts originate upstream of glnA; one reading through into ntrBC and one terminating at a sequence resembling a rho-independent terminator between glnA and ntrBC. A third transcript originates from the ntrBC promoter which has a consensus binding site for the ntrC product in the -10 region. Comparison of the glnA-ntrBC intergenic sequences from K. pneumoniae, Escherichia coli and Salmonella typhimurium has identified a number of conserved features and some significant differences.
mesh_terms: D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000302:isolation & purification; D004720:Endonucleases; D029968:Escherichia coli Proteins / Q000235:genetics* / Q000302:isolation & purification; D005796:Genes; D005798:Genes, Bacterial; D005809:Genes, Regulator; D007711:Klebsiella pneumoniae / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009097:Multienzyme Complexes / Q000235:genetics* / Q000302:isolation & purification; D009586:Nitrogen Fixation; D010749:Phosphoprotein Phosphatases / Q000235:genetics* / Q000302:isolation & purification; D011401:Promoter Regions, Genetic; D011494:Protein Kinases / Q000235:genetics* / Q000302:isolation & purification; D012689:Sequence Homology, Nucleic Acid; D015719:Single-Strand Specific DNA and RNA Endonucleases; D013045:Species Specificity; D014158:Transcription, Genetic
chemical_list: D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D009097:Multienzyme Complexes; C578385:glnL protein, E coli; D011494:Protein Kinases; D004720:Endonucleases; D004262:DNA Restriction Enzymes; D010749:Phosphoprotein Phosphatases; D015719:Single-Strand Specific DNA and RNA Endonucleases