[Manifestation of instability in the leucine-dependent Bacillus subtilis auxotroph induced by herring DNA].
Unstable leucine-depending Bacillus subtilis auxotroph previously induced by the herring DNA was studied. Instability was manifested as revertability in the leucine-free medium. Average frequency of revertants amounted to 50% of the number of viable cells. A clone analysis has shown that most of such colonies are mosaics consisting of leucine-independent and leucine-sensitive cells. Unstable mutation was cotransformed with leuA marker. Heating followed by material storage in demineralized water stimulated transposition of unstable mutation under study to the tryptophan operon region.
D000818:Animals; D001412:Bacillus subtilis / Q000235:genetics* / Q000254:growth & development; D003470:Culture Media; D004247:DNA / Q000235:genetics*; D004251:DNA Transposable Elements; D005399:Fishes; D007930:Leucine / Q000494:pharmacology; D009154:Mutation; D014169:Transformation, Bacterial
D003470:Culture Media; D004251:DNA Transposable Elements; D004247:DNA; D007930:Leucine
Proiavlenie nestabil'nosti u leĭtsinzavisimogo auksotrofa Bacillus subtilis, poluchennogo s pomoshch'iu DNK sel'di.