[Evaluation of the size of the continuous poly(G) site necessary for the biological activity of the poly(G).poly(C) complex].
On the basis of synthesis of a series of poly(G, A).poly(C) copolymers with changing G:A ratio from 15:1 to 90:1 and trials of their biological activity in comparison with poly(G).poly(C), the size of poly(G) in it was evaluated within the range of a continuous double-stranded area necessary for the activity. The antiviral activity close to that of poly(G).poly(C) in experimental tick-borne encephalitis of mice and vesicular stomatitis virus infection of chick embryo cells was found only in poly(G,A).poly(C) complexes with a G:A ratio equal to or higher than 90:1. Consequently, the high activity of poly(G).poly(C) is present at an average length of poly(G) equal to 90-100 nucleotides within the limits of the continuous double-stranded area.
D000818:Animals; D001483:Base Sequence; D002642:Chick Embryo; D004353:Drug Evaluation, Preclinical; D004675:Encephalitis, Tick-Borne / Q000188:drug therapy; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D011066:Poly C / Q000032:analysis / Q000627:therapeutic use*; D011068:Poly G / Q000032:analysis / Q000627:therapeutic use*; D011131:Polyribonucleotides / Q000032:analysis / Q000627:therapeutic use*; D013329:Structure-Activity Relationship; D014721:Vesicular stomatitis Indiana virus / Q000187:drug effects; D010948:Viral Plaque Assay
D011131:Polyribonucleotides; D011068:Poly G; C008961:poly G-poly C; D011066:Poly C; C042481:poly(G,A).poly(C)
Otsenka razmera nepreryvnogo uchastka poli(G), neobkhodimogo dlia biologicheskoĭ aktivnosti kompleksa (G).poli(Ts).