Effect of nucleotides on glucan synthesis in Paracoccidioides brasiliensis.
The activity of Paracoccidioides brasiliensis glucan synthetase was partially inhibited by guanosine 5'-triphosphate, adenosine 3'5'-phosphate and adenosine 5'-triphosphate. This inhibition was more pronounced in the mycelial system than in the yeast one, being higher at 23 degrees C than at 37 degrees C. Addition of ethene diamine tetracetic acid to the incubation mixture inhibited partially the enzymatic activity in mycelial preparations but stimulated it in the yeast system.
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000242:Cyclic AMP / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D005936:Glucans / Q000096:biosynthesis*; D005964:Glucosyltransferases / Q000037:antagonists & inhibitors*; D006160:Guanosine Triphosphate / Q000494:pharmacology; D003904:Mitosporic Fungi / Q000378:metabolism*; D009711:Nucleotides / Q000494:pharmacology*; D010228:Paracoccidioides / Q000201:enzymology / Q000378:metabolism*; D013696:Temperature
D005936:Glucans; D009711:Nucleotides; D006160:Guanosine Triphosphate; D000255:Adenosine Triphosphate; D004492:Edetic Acid; D000242:Cyclic AMP; D005964:Glucosyltransferases; C040232:glucan synthase