Membrane fatty acids, lipid peroxidation and adenylate cyclase activity in cultured neural cells.
Lipid peroxidation and basal adenylate cyclase activity have been examined in neuroblastoma cultured with a variety of exogenous fatty acids. Formation of cyclic AMP depended upon fatty acid type, with supplementation affecting activities in the order: linoleate greater than cis-vaccenate = linolenate greater than control (132.7, 72.6, 71.9 and 36.0 pmol cAMP formed/mg protein, respectively). Lipid peroxidation, measured by formation of malondialdehyde (MDA), also varied with fatty acid; however, there was little correlation between MDA production and basal cyclase activity. Inclusion of alpha-tocopherol in culture-medium blocked MDA formation without affecting cAMP accumulation. Fe2+-dependent induction of peroxidation was accompanied by a time-dependent inhibition of cyclase activity.
D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000096:biosynthesis; D005227:Fatty Acids / Q000378:metabolism*; D005231:Fatty Acids, Unsaturated / Q000378:metabolism; D008054:Lipid Peroxides / Q000096:biosynthesis*; D008563:Membrane Lipids / Q000378:metabolism*; D051379:Mice; D009447:Neuroblastoma / Q000378:metabolism*; D010084:Oxidation-Reduction; D014810:Vitamin E / Q000494:pharmacology
D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D008054:Lipid Peroxides; D008563:Membrane Lipids; D014810:Vitamin E; D000242:Cyclic AMP; D000262:Adenylyl Cyclases