An assay for proteolytic activity using spin-labeled substrates.
A method is proposed for the assay of proteolytic activity based on the measurement of changes in the electron spin resonance spectra (increase in the ratio of weakly to strongly immobilized spin label residues) of substrate proteins labeled with a maleimide nitroxide derivative.
D000818:Animals; D002417:Cattle; D002918:Chymotrypsin / Q000378:metabolism*; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods; D006454:Hemoglobins / Q000378:metabolism; D007700:Kinetics; D012710:Serum Albumin, Bovine / Q000378:metabolism; D013113:Spin Labels; D014357:Trypsin / Q000378:metabolism*
D006454:Hemoglobins; D013113:Spin Labels; D012710:Serum Albumin, Bovine; D002918:Chymotrypsin; D014357:Trypsin