title: Vasoregulin, a glucocorticoid-inducible vascular permeability inhibitory protein.
abstract: A vascular permeability inhibitory protein 'Vasoregulin' was induced by dexamethasone and other glucocorticoids in Namalva cells. Precipitates produced by trichloroacetic acid from cultured supernatant (crude released vasoregulin) suppressed serotonin-induced paw edema of mice (ID30 = 750 micrograms/mouse) when dosed 30 min. before serotonin. Carrageenan paw edema of rats was also suppressed (ID30 = 9 mg/rat, s.c. and i.p., not by oral dosing) when the compound was injected at the same time as carrageenan and the edema determined after 3 hrs. The possible effect of glucocorticoid itself being contained in the vasoregulin preparation was excluded. The presence of Cu, Zn-superoxide dismutase (SOD), spermidine and spermine also gave good yields of vasoregulin in culture; SOD protected the inactivation of vasoregulin from superoxide radical in vitro. Vasoregulin partially purified by Sephadex G-75 columns had no malonaldehyde-formation inhibitory capacity. Thus, vasoregulin is a glucocorticoid-inducible anti-inflammatory protein which differs from lipocortin.
mesh_terms: D000818:Animals; D002199:Capillary Permeability / Q000187:drug effects*; D002351:Carrageenan; D002478:Cells, Cultured; D004487:Edema / Q000503:physiopathology; D005938:Glucocorticoids / Q000494:pharmacology*; D006801:Humans; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009363:Neoplasm Proteins; D010741:Phospholipases A / Q000037:antagonists & inhibitors; D011453:Prostaglandins / Q000096:biosynthesis; D011506:Proteins / Q000032:analysis / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D012701:Serotonin; D013481:Superoxides / Q000378:metabolism; D013997:Time Factors
chemical_list: D005938:Glucocorticoids; D009363:Neoplasm Proteins; D011453:Prostaglandins; D011506:Proteins; C048490:vasoregulin; D013481:Superoxides; D012701:Serotonin; D002351:Carrageenan; D010741:Phospholipases A