Mixed-mode hydrophobic ion exchange for the separation of oligonucleotides and DNA fragments using HPLC.
Hydrophobic anion exchangers were formed by cobonding both ionic and hydrophobic ligands to silica gel. These phases were used to separate single-stranded oligonucleotides and double-stranded DNA restriction fragments. By varying the ratio of n-octyldimethylsilane and either 3-chloropropyldimethylsilane or 4-chlorobutyldimethylsilane added during silanization a series of mixed-ligand or mixed-mode stationary phases was created. Concentration and ratio of bonded ligands were determined using a new gas chromatography fluorination method. Total ligand coverage was found to approach 2.1 ligands nm-2 for n-octyldimethylsilane. Bonding reproducibility for mixed-mode phases was good. Nucleic acid separations were achieved under gentle mobile phase conditions by using the stationary phase as an easily modifiable variable.
D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002852:Chromatography, Ion Exchange; D004247:DNA / Q000302:isolation & purification*; D004262:DNA Restriction Enzymes; D004277:DNA, Single-Stranded / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D007700:Kinetics; D008024:Ligands; D009841:Oligonucleotides / Q000302:isolation & purification*
D004277:DNA, Single-Stranded; D008024:Ligands; D009841:Oligonucleotides; D004247:DNA; D004262:DNA Restriction Enzymes