A critical base in the yeast mitochondrial nonanucleotide promoter. Abolition of promoter activity by mutation at the -2 position.
The yeast mitochondrial promoter comprises a highly conserved nonanucleotide sequence, 5'-ATATAAGTA-3'. Transcription is initiated both in vivo and in vitro at the 3'-terminal A (+1). The G at the -2 position is strictly conserved. The requirement for this nucleotide is established by comparing the in vitro promoter activity of nonanucleotides containing any of the four bases at the -2 position, obtained as synthetic oligonucleotides. Only the synthetic promoter having G at the -2 position exhibits any promoter activity.
D001483:Base Sequence; D004262:DNA Restriction Enzymes / Q000378:metabolism; D015247:Deoxyribonuclease HindIII; D015252:Deoxyribonucleases, Type II Site-Specific; D009154:Mutation; D009876:Operon; D012335:RNA, Ribosomal / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014158:Transcription, Genetic
D012335:RNA, Ribosomal; D004262:DNA Restriction Enzymes; D015247:Deoxyribonuclease HindIII; C032824:ATCGAT-specific type II deoxyribonucleases; C025630:CAGCTG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific