Mutagenesis of the avian erythroblastosis virus erbB coding region: an intact extracellular domain is not required for oncogenic transformation.
Avian erythroblastosis virus (AEV) is an oncogenic retrovirus of birds. The AEV-encoded erbB polypeptide, a transmembrane glycoprotein bearing an N-terminal domain exposed on the surface of virally transformed cells, plays a crucial role in AEV-mediated oncogenesis. We report here a characterization of a mutated form of the AEV erbB protein which lacks over two-thirds of the extracellular region of this oncogenic protein. This mutant v-erbB protein, although lacking the three possible extracellular sites of N-linked protein glycosylation, appears unimpaired in the ability to transform cells to an oncogenic phenotype.
D001355:Alpharetrovirus / Q000235:genetics*; D000818:Animals; D001354:Avian Leukosis Virus / Q000235:genetics*; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D002642:Chick Embryo; D002872:Chromosome Deletion; D004912:Erythrocytes / Q000382:microbiology; D005110:Extracellular Space; D008565:Membrane Proteins / Q000235:genetics; D008970:Molecular Weight; D009154:Mutation; D009856:Oncogene Proteins, Viral / Q000235:genetics*; D009857:Oncogenes; D013329:Structure-Activity Relationship
D008565:Membrane Proteins; D009856:Oncogene Proteins, Viral