Transformation of quail embryo fibroblasts by a retrovirus carrying a normal human c-myc gene.
We have constructed avian retroviruses expressing the human c-myc oncogene. These viruses morphologically transformed primary quail embryo fibroblasts upon transfection and infection. Transformed cells produced viruses harboring a spliced c-myc gene and contained high levels of p64-67c-myc protein. One of these infectious viruses, vSX-AHM, was molecularly cloned and the nucleotide sequence of the spliced c-myc insert determined. No mutation was found within the c-myc coding sequence of this transforming clone when compared to the normal genomic progenitor. Thus, we concluded that no mutation within the human c-myc gene is required to induce primary avian embryo fibroblast transformation.
D000818:Animals; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D003001:Cloning, Molecular; D003370:Coturnix; D004262:DNA Restriction Enzymes; D004625:Embryo, Nonmammalian; D006801:Humans; D009857:Oncogenes; D010957:Plasmids; D012190:Retroviridae / Q000235:genetics*
D004262:DNA Restriction Enzymes