title: Mechanism of adenosine production by isolated rat heart mitochondria.
abstract: Adenosine production by isolated rat heart mitochondria was examined and was observed to be dependent on an active adenine nucleotide transporter and a functional 5'-nucleotidase. It was found that mitochondria do not transport adenosine. These results suggest that mitochondria provide AMP for an extramitochondrial 5'-nucleotidase and this was verified by direct measurement of extramitochondrial levels of AMP and adenosine. A possible role for mitochondria in myocardial adenosine production is discussed.
mesh_terms: D015720:5'-Nucleotidase; D000241:Adenosine / Q000096:biosynthesis*; D000244:Adenosine Diphosphate / Q000378:metabolism; D000249:Adenosine Monophosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001692:Biological Transport; D066298:In Vitro Techniques; D008297:Male; D008929:Mitochondria, Heart / Q000378:metabolism*; D009708:Nucleotidases / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D000249:Adenosine Monophosphate; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D009708:Nucleotidases; D015720:5'-Nucleotidase; D000241:Adenosine