The selective induction of a small number of proteins during G1 transit results from the mitogenic action of pp60v-src in tsASV-infected rat cells.
Since the mitogenic/oncogenic pp60v-src product of the avian sarcoma virus (ASV) mutant, tsLA23, is abnormally thermolabile, tsLA23-NRK cells were phenotypically nontransformed at 40 degrees C and were consequently rendered quiescent by serum deprivation at this temperature. These serum-deprived cells were stimulated to transit G1 either as transformed cells by simply dropping the temperature to a pp60v-src -activating 36 degrees C, or as nontransformed cells by adding serum at 40 degrees C. Serum stimulation rapidly increased total protein synthesis in these cells and over 100 changes in cellular proteins (resolved by two-dimensional gel electrophoresis) occurred during G1 transit. By contrast, pp60v-src-activation did not increase total protein synthesis and only six proteins (18.5-44 kD) were clearly seen to appear or increase when quiescent cells were stimulated to transit G1 by activating pp60v-src. Three of these six pp60v-src- induced proteins also appeared or accumulated during the G1 transit of serum-stimulated cells. The appearance and/or accumulation of the six proteins and the subsequent initiation of DNA replication may have resulted from pp60v-src stimulating only a small number of critical cellular genes because both the protein changes and DNA replication were completely suppressed by the transcription inhibitor actinomycin D.
D000818:Animals; D001358:Avian Sarcoma Viruses / Q000235:genetics*; D002472:Cell Transformation, Viral; D003609:Dactinomycin / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D007399:Interphase; D007668:Kidney / Q000166:cytology; D008715:Methionine / Q000378:metabolism; D008970:Molecular Weight; D009154:Mutation; D015688:Oncogene Protein pp60(v-src); D051381:Rats; D012191:Retroviridae Proteins / Q000494:pharmacology*; D013696:Temperature; D013997:Time Factors
D012191:Retroviridae Proteins; D003609:Dactinomycin; D008715:Methionine; D015688:Oncogene Protein pp60(v-src)