Transverse motion of spin-labeled 3,3',5-triiodo-L-thyronine in phospholipid bilayers.
Using electron spin resonance stop-flow technique, the transverse motion (flip-flop) of 3-([alpha-carboxy-4-(4-hydroxy-3-iodophenoxy)-3,5- diiodophenethyl]carbamoyl)-2,2,5,5-tetramethyl-3-pyrrolin (T3-SL) in dipalmitoyl L-alpha-phosphosphatidylcholine (DPPC) membranes was evaluated. At 22 degrees C, the electron spin resonance spectra of T3-SL in DPPC vesicles were compared before and after the addition of sodium ascorbate, a membrane impermeable reducing agent. The addition of ascorbate reduces the signal amplitude by 67% in 3 min but yields no further reduction for at least 60 min. These results indicate that T3-SL does not flip-flop at any appreciable rate in the membranes. This finding suggests that once partitioned into the membrane, T3 remains in the outer half of the lipid bilayer, thus reducing the possibility that T3 enters the cell by passive diffusion.
D001205:Ascorbic Acid / Q000494:pharmacology; D003497:Cyclic N-Oxides / Q000378:metabolism*; D004058:Diffusion; D004578:Electron Spin Resonance Spectroscopy; D008051:Lipid Bilayers / Q000378:metabolism*; D008081:Liposomes / Q000378:metabolism; D011663:Pulmonary Surfactants; D013113:Spin Labels; D013696:Temperature; D014284:Triiodothyronine / Q000031:analogs & derivatives
D003497:Cyclic N-Oxides; D008051:Lipid Bilayers; D008081:Liposomes; D011663:Pulmonary Surfactants; D013113:Spin Labels; D014284:Triiodothyronine; C042048:3-((alpha-carboxy-4-(4-hydroxy-3-iodophenoxy)-3,5-diiodophenethyl)carbamoyl)-2,2,5,5-tetramethyl-3-pyrrolin-1-yloxy; D001205:Ascorbic Acid