Isolation and characterization of genomic clones for two chicken phenobarbital-inducible cytochrome P-450 genes.
A cDNA clone specific for a chicken phenobarbital-inducible cytochrome P-450 was used to screen a chicken genomic library. Twenty-nine clones were isolated, restriction mapped, and divided into two non-overlapping groups. The cDNA clone hybridized to 12 kilobases of DNA from both groups. Both groups contained restriction fragments which hybridized to both 5' and 3' fragments of the cDNA clone, and it was concluded that the two groups were derived from two separate genes. Southern transfer analysis of individual chicken DNAs and quantitative hybridization analysis indicated that these two genes are independent and are present as single copies/haploid genome. Comparison of restriction digests of the cloned DNAs and total genomic DNA discounted the possibility that other closely related P-450 genes are present in the chicken genome.
D000818:Animals; D010582:Bacteriophage lambda / Q000235:genetics; D002645:Chickens; D003101:Collodion; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant / Q000302:isolation & purification*; D015245:Deoxyribonuclease BamHI; D015246:Deoxyribonuclease EcoRI; D004591:Electrophoresis, Polyacrylamide Gel; D009693:Nucleic Acid Hybridization; D010634:Phenobarbital / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics
D004274:DNA, Recombinant; D012333:RNA, Messenger; D003101:Collodion; D004247:DNA; D003577:Cytochrome P-450 Enzyme System; D004262:DNA Restriction Enzymes; D015245:Deoxyribonuclease BamHI; D015246:Deoxyribonuclease EcoRI; D010634:Phenobarbital