Glycerol-3-phosphate dehydrogenase mRNA content in cultured 3T3-L1 adipocytes: regulation by dibutyryl cyclic AMP.
Incubation of differentiated 3T3-L1 adipocytes with 0.5 mM dibutyryl cAMP plus 0.5 mM theophylline for 2 hours results in an 85% decrease in glycerol-3-phosphate dehydrogenase (G3PD) mRNA content. Incubation of the adipocytes with insulin (1 microgram/ml) up to 24 hrs yielded no significant change in the G3PD mRNA content compared with control.
D000273:Adipose Tissue / Q000201:enzymology*; D000818:Animals; D003994:Bucladesine / Q000494:pharmacology*; D002454:Cell Differentiation; D002460:Cell Line; D005786:Gene Expression Regulation / Q000187:drug effects*; D005993:Glycerolphosphate Dehydrogenase / Q000235:genetics*; D007328:Insulin / Q000494:pharmacology; D051379:Mice; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000378:metabolism*
D007328:Insulin; D012333:RNA, Messenger; D003994:Bucladesine; D005993:Glycerolphosphate Dehydrogenase