Oxidizing intermediates in the reaction of ferrous EDTA with hydrogen peroxide. Reactions with organic molecules and ferrocytochrome c.
The reaction between hydrogen peroxide and ferrous EDTA generates an oxidizing intermediate (I1) which is not the hydroxyl radical. It oxidizes ferrocytochrome c and also reacts with hydrogen peroxide (k5 = 3.2 X 10(3) M-1 S-1) to form a second oxidizing transient (I2). I1 is not scavenged by t-butyl alcohol whereas I2 is. I1 is found to be significantly less reactive than the hydroxyl radical toward benzoate ion, t-butyl alcohol, acetate ion, arginine, and serine, but is scavenged by compounds with readily oxidizable functional groups such as ethanol and isopropyl alcohol. This indicates that I1 does not undergo the characteristic reactions of the hydroxyl radical but shows a pattern of reactivity more associated with a metal ion oxidant like a ferryl (FeO2+)-EDTA complex.
D000596:Amino Acids; D003574:Cytochrome c Group / Q000378:metabolism*; D004492:Edetic Acid; D005296:Ferrous Compounds; D005609:Free Radicals; D006861:Hydrogen Peroxide; D006878:Hydroxides; D017665:Hydroxyl Radical; D007501:Iron; D007502:Iron Chelating Agents; D007700:Kinetics; D010084:Oxidation-Reduction; D013053:Spectrophotometry
D000596:Amino Acids; D003574:Cytochrome c Group; D005296:Ferrous Compounds; D005609:Free Radicals; D006878:Hydroxides; D007502:Iron Chelating Agents; D017665:Hydroxyl Radical; D004492:Edetic Acid; D006861:Hydrogen Peroxide; D007501:Iron