Expression of the human c-fms proto-oncogene product (colony-stimulating factor-1 receptor) on peripheral blood mononuclear cells and choriocarcinoma cell lines.
The c-fms gene product is related, and possibly identical, to the receptor for the mononuclear phagocyte colony stimulating factor, CSF-1. Using antisera to a recombinant v-fms--coded polypeptide, glycoproteins encoded by the human c-fms locus were detected in mononuclear cells from normal peripheral blood and in promyelocytic HL-60 cells 24 h after induction of monocytic differentiation with phorbol ester. The 150-kD human c-fms--coded glycoprotein was expressed at the cell surface, was active as a tyrosine-specific protein kinase in vitro, and shared primary structural features with the product of the feline retroviral v-fms oncogene. A biochemically indistinguishable glycoprotein was detected in human choriocarcinoma cell lines. Like peripheral blood mononuclear cells and phorbol ester-treated HL-60 cells, the choriocarcinoma cells expressed high affinity binding sites for human CSF-1. In addition to serving as a lineage specific growth factor in hematopoiesis, CSF-1 may play a role in normal trophoblast development.
D000818:Animals; D002460:Cell Line; D002822:Choriocarcinoma / Q000378:metabolism*; D005260:Female; D006023:Glycoproteins / Q000096:biosynthesis; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000378:metabolism; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism*; D009856:Oncogene Proteins, Viral / Q000096:biosynthesis; D011247:Pregnancy; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis; D011519:Proto-Oncogenes; D011817:Rabbits; D011956:Receptors, Cell Surface / Q000096:biosynthesis*; D016184:Receptors, Colony-Stimulating Factor; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014594:Uterine Neoplasms / Q000378:metabolism*
D006023:Glycoproteins; C000715470:MAS1 protein, human; D009856:Oncogene Proteins, Viral; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins; D011956:Receptors, Cell Surface; D016184:Receptors, Colony-Stimulating Factor; D013755:Tetradecanoylphorbol Acetate