Fucosterol decreases angiotensin converting enzyme levels with reduction of glucocorticoid receptors in endothelial cells.
The modulation of angiotensin converting enzyme (ACE) levels was studied using fucosterol, one of phytosterols, in cultured bovine carotid endothelial cells. Addition of fucosterol to the culture medium resulted in the decrease of ACE activity of endothelial cells; however, fucosterol did not directly inhibit ACE activity. Dexamethasone elevated the levels of ACE in normal cells, but this effect was not seen in the fucosterol-treated cells. Receptor assays showed that the amount of glucocorticoid receptors in fucosterol-treated cells decreased to an undetectable level. These results indicate that fucosterol lowers the ACE levels on the endothelial cells by inhibiting the synthesis of glucocorticoid receptors involved in the regulation of ACE levels.
D002478:Cells, Cultured; D003907:Dexamethasone / Q000494:pharmacology; D004727:Endothelium / Q000032:analysis* / Q000187:drug effects; D007703:Peptidyl-Dipeptidase A / Q000032:analysis*; D010840:Phytosterols / Q000494:pharmacology*; D011965:Receptors, Glucocorticoid / Q000032:analysis*; D012855:Sitosterols / Q000494:pharmacology; D013265:Stigmasterol / Q000031:analogs & derivatives / Q000494:pharmacology*
D010840:Phytosterols; D011965:Receptors, Glucocorticoid; D012855:Sitosterols; C015896:fucosterol; C025473:gamma-sitosterol; D003907:Dexamethasone; D013265:Stigmasterol; D007703:Peptidyl-Dipeptidase A