Differential effects of phorbol ester on the beta-adrenergic response of normal and ras-transformed NIH3T3 cells.
The basal and isoproterenol stimulated levels of cyclic AMP in NIH3T3 and H-ras transformed NIH3T3 cells were equivalent. In exponentially growing cells, the phorbol ester 12-O-tetradecanoyl-13-acetate (TPA) inhibited the beta-adrenergic response of NIH3T3 cells, but not of the ras-transformed line. Another line of NIH3T3 cells transformed by a non-ras gene exhibited the normal loss of beta-adrenergic response by the tumor promoter. These results are consistent with a role for p21 in signal transduction related to the effects of TPA.
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000378:metabolism*; D000242:Cyclic AMP / Q000378:metabolism; D004273:DNA, Neoplasm / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D007545:Isoproterenol / Q000494:pharmacology; D051379:Mice; D008819:Mice, Nude; D009857:Oncogenes; D010641:Phenotype; D010704:Phorbols / Q000494:pharmacology*; D011943:Receptors, Adrenergic, beta / Q000187:drug effects*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D001749:Urinary Bladder Neoplasms / Q000235:genetics
D004273:DNA, Neoplasm; D010704:Phorbols; D011943:Receptors, Adrenergic, beta; D000242:Cyclic AMP; D007545:Isoproterenol; D013755:Tetradecanoylphorbol Acetate