Primate cytomegalovirus glycoproteins: lectin-binding properties and sensitivities to glycosidases.
The lectin-binding properties and glycosidase sensitivities of the virion glycoproteins of primate cytomegaloviruses (CMVs) were examined. Three simian CMV (SCMV) strains, including Colburn, and four human CMV (HCMV) strains were compared. Their proteins were separated in denaturing polyacrylamide gels and electrotransferred onto nitrocellulose, and the glycosylated species were visualized with iodinated concanavalin A or wheat germ agglutinin (WGA). Virions of both HCMV and SCMV strains contained six principal and several minor lectin-reactive bands. Neuraminidase treatment abolished WGA binding and reduced the charge and charge heterogeneity of the SCMV (i.e., Colburn) virion glycoproteins and had a similar, although less dramatic, effect on those of HCMV. The specificities of concanavalin A and WGA in these assays were evaluated with endo-beta-N-acetylglucosaminidase H and endo-beta-N-acetylglucosaminidase F, and a combination of lectins and glycosidases was used to demonstrate that many of the primate CMV glycoproteins contain both high-mannose and complex, N-linked oligosaccharides. Results suggest that the HCMV virion glycoproteins are more extensively glycosylated or have more completely processed carbohydrate side chains, or both, than their SCMV counterparts.
D000118:Acetylglucosaminidase / Q000378:metabolism; D000818:Animals; D003208:Concanavalin A / Q000378:metabolism; D003587:Cytomegalovirus / Q000032:analysis*; D006023:Glycoproteins / Q000032:analysis* / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000378:metabolism; D000882:Haplorhini; D006801:Humans; D037102:Lectins / Q000378:metabolism; D008358:Mannose / Q000032:analysis; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase; D009439:Neuraminidase / Q000378:metabolism; D009844:Oligosaccharides / Q000032:analysis; D010455:Peptides / Q000032:analysis; D014764:Viral Proteins / Q000032:analysis* / Q000378:metabolism; D014909:Wheat Germ Agglutinins
D006023:Glycoproteins; D037102:Lectins; D009844:Oligosaccharides; D010455:Peptides; D014764:Viral Proteins; D014909:Wheat Germ Agglutinins; D003208:Concanavalin A; D006026:Glycoside Hydrolases; D009439:Neuraminidase; D000118:Acetylglucosaminidase; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase; D008358:Mannose