Simultaneous synthesis and hydrolysis of ATP regulated by the inhibitor protein in submitochondrial particles.
Coupled submitochondrial particles from bovine heart with ATP synthases devoid of control by the inhibitor protein of Pullman and Monroy [J. Biol. Chem. 238, 3762-3769 (1963)] can be prepared by incubation of Mg-ATP particles in 50 mM phosphate, 250 mM sucrose, and greater than 95% D2O (pD 7.8) at 38 degrees C. As monitored with oxonol, the respiring particles build up and maintain a delta psi about 5-10% lower than that of the starting preparation. With oligomycin delta psi of the two preparations is the same. In the presence of an ATP trap (hexokinase and glucose), the two types of particles carry out oxidative phosphorylation at comparable rates. Low concentrations of oligomycin induce a small enhancement of the rate of ATP synthesis in non-controlled particles. In the absence of an ATP trap, net accumulation of ATP, as driven by electron transport in particles without control by the inhibitor protein, is low. Apparently this is due to lack of control by the inhibitor protein of ATP hydrolysis that occurs during oxidative phosphorylation.
D025181:ATP Synthetase Complexes; D000255:Adenosine Triphosphate / Q000096:biosynthesis / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D006868:Hydrolysis; D066298:In Vitro Techniques; D008929:Mitochondria, Heart / Q000378:metabolism; D009097:Multienzyme Complexes / Q000037:antagonists & inhibitors*; D010085:Oxidative Phosphorylation; D010770:Phosphotransferases / Q000037:antagonists & inhibitors*; D011506:Proteins / Q000378:metabolism*; D013367:Submitochondrial Particles / Q000378:metabolism*; D000097795:ATPase Inhibitory Protein
D025181:ATP Synthetase Complexes; D000255:Adenosine Triphosphate; D009097:Multienzyme Complexes; D010770:Phosphotransferases; D011506:Proteins; D000097795:ATPase Inhibitory Protein