Phenylmethylsulfonyl fluoride (PMSF) inhibits nerve growth factor binding to the high affinity (type I) nerve growth factor receptor.
Dorsal root ganglia were extirpated from 9-day old embryonic chickens and solubilized in phosphate buffered saline containing 0.5% Noniodet P 40 detergent. When nerve growth factor binding studies are performed on these samples, the expected curvilinear Rosenthal (Scatchard) plot is obtained. However, when the solubilized cell sample is made 1-2 mM in phenylmethylsulfonyl fluoride and nerve growth factor binding is determined, a linear Rosenthal (Scatchard) plot is obtained. The equilibrium dissociation constant obtained from the slope of the line is 1.9 X 10(-9) M, identical to the equilibrium dissociation constant of the low affinity receptor. A similar phenomenon is observed when rat pheochromocytoma cells are solubilized in the non-ionic detergent and nerve growth factor binding is determined. No high affinity binding can be detected for either cell type when detergent solubilized cells are incubated with phenylmethylsulfonyl fluoride.
D000818:Animals; D002642:Chick Embryo; D004228:Dithionitrobenzoic Acid / Q000494:pharmacology; D004815:Epidermal Growth Factor / Q000378:metabolism*; D066246:ErbB Receptors; D005727:Ganglia, Spinal / Q000378:metabolism; D066298:In Vitro Techniques; D007700:Kinetics; D010664:Phenylmethylsulfonyl Fluoride / Q000494:pharmacology*; D011956:Receptors, Cell Surface / Q000187:drug effects / Q000378:metabolism*; D012694:Serine / Q000378:metabolism; D012995:Solubility; D013450:Sulfones / Q000494:pharmacology*
D011956:Receptors, Cell Surface; D013450:Sulfones; D012694:Serine; D010664:Phenylmethylsulfonyl Fluoride; D004815:Epidermal Growth Factor; D004228:Dithionitrobenzoic Acid; D066246:ErbB Receptors