Effects of diabetes on fructose 2, 6-P2, glucose 1, 6-P2 and 6-phosphofructo 2-kinase in rat liver.
The levels of fructose 2,6-P2 and 6-phosphofructo 2-kinase have been found to be decreased in the liver of both ketotic and non-ketotic diabetic rats, a good correlation between fall of hepatic fructose 2,6-P2, ketonemia and glycemia being observed. The "total" 6-phosphofructo 2-kinase activity and the "active" (non-phosphorylated) from of the enzyme were decreased to a different extent, resulting in a fall of the "active"/"total" activity ratio. Hepatic levels of glucose 1,6-P2 were lowered only in ketotic diabetes. Insulin treatment normalized all the values studied. Insulin administration to control rats decreased the hepatic levels of fructose 2,6-P2 and did not affect glucose 1,6-P2 levels. It also decreased the "active" form of 6-phosphofructo 2-kinase, without significantly altering the "total" activity.
D020155:3-Hydroxybutyric Acid; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy / Q000378:metabolism*; D016883:Diabetic Ketoacidosis / Q000378:metabolism; D004789:Enzyme Activation; D005635:Fructosediphosphates / Q000378:metabolism*; D019298:Glucose-6-Phosphate / Q000031:analogs & derivatives*; D005958:Glucosephosphates / Q000378:metabolism*; D006598:Hexosediphosphates / Q000378:metabolism*; D006885:Hydroxybutyrates / Q000097:blood; D007328:Insulin / Q000627:therapeutic use; D008099:Liver / Q000378:metabolism*; D008297:Male; D025481:Phosphofructokinase-2; D010766:Phosphorylation; D010770:Phosphotransferases / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D001786:Blood Glucose; D005635:Fructosediphosphates; D005958:Glucosephosphates; D006598:Hexosediphosphates; D006885:Hydroxybutyrates; D007328:Insulin; D019298:Glucose-6-Phosphate; C027652:fructose 2,6-diphosphate; C016473:glucose-1,6-bisphosphate; D010770:Phosphotransferases; D025481:Phosphofructokinase-2; D020155:3-Hydroxybutyric Acid