Sequence analysis of a Dictyostelium discoideum gene coding for an active dihydroorotate dehydrogenase in yeast.
A Dictyostelium discoideum DNA fragment isolated on the basis of its ability to complement the ural mutation of yeast, codes for a dihydroorotate dehydrogenase activity. The complete nucleotide sequence of this 1898 bp fragment has been determined and reveals an open reading frame capable of coding for a 369 amino acid polypeptide of molecular mass 47.000. The gene shows preferential use of codons with weak pairing forces. Eleven codons, mainly those with a G in the third position, are absent. The flanking sequences are unusually rich in A + T (80%). Several direct and inverted repeats exist in the 5' flanking sequence.
D001483:Base Sequence; D003062:Codon; D004274:DNA, Recombinant; D004023:Dictyostelium / Q000235:genetics*; D004081:Dihydroorotate Oxidase / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D009154:Mutation; D009693:Nucleic Acid Hybridization; D010088:Oxidoreductases / Q000235:genetics*; D010957:Plasmids; D012091:Repetitive Sequences, Nucleic Acid; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D003062:Codon; D004274:DNA, Recombinant; D010088:Oxidoreductases; D004081:Dihydroorotate Oxidase