Improved lipid preservation by malachite green-glutaraldehyde fixation in rat incisor predentine and dentine.
A network of granules and filaments stained with malachite green-aldehyde (MGA) was observed in predentine in the spaces between the collagen fibres and many small granules were associated with these fibres in peripheral and circumpulpal-dentine. The granules were present throughout the entire thickness of the dentine, especially at the periphery of the calcospherites, although scarce or absent at their centre. This MGA-stainable material (i) resisted demineralization in acetic acid and EDTA solutions, and (ii) differed from non-collagenous material and crystal ghosts which stained on control sections after prior demineralization. In predentine, transport and diffusion of MGA-stainable material occurred in the direction of the dentine in which this material was associated with periodical striation of mineralized collagen fibres. This suggests that true matrix-associated lipids might play a role in dentine mineralization.
D000447:Aldehydes; D000818:Animals; D003094:Collagen / Q000032:analysis; D003804:Dentin / Q000648:ultrastructure*; D005404:Fixatives; D005976:Glutaral; D006651:Histocytochemistry; D007180:Incisor; D002479:Inclusion Bodies / Q000648:ultrastructure; D008055:Lipids / Q000032:analysis*; D008854:Microscopy, Electron; D051381:Rats; D011919:Rats, Inbred Strains; D012394:Rosaniline Dyes
D000447:Aldehydes; D005404:Fixatives; D008055:Lipids; D012394:Rosaniline Dyes; C005095:malachite green; D003094:Collagen; D005976:Glutaral