title: Partial purification of a 5.7K glycoprotein from bovine vitreous which inhibits both angiogenesis and collagenase activity.
abstract: An inhibitor of angiogenic activity similar to that described by Raymond and Jacobson (1) has been partially purified and shown to be a glycoprotein of molecular mass 5,700. This inhibitor gave rise to an avascular zone on the chick vitelline plexus and also negated the action of a low Mr angiogenic factor. When the angiogenic inhibitor was incubated with mammalian collagenase it inhibited the enzyme activity by nearly 70%. The relevance of these findings to the role of collagenase in angiogenesis is discussed.
mesh_terms: D043925:Angiogenesis Inducing Agents / Q000037:antagonists & inhibitors; D020533:Angiogenesis Inhibitors; D000818:Animals; D002417:Cattle; D002642:Chick Embryo; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D005136:Eye Proteins / Q000302:isolation & purification* / Q000494:pharmacology; D005347:Fibroblasts / Q000201:enzymology; D006023:Glycoproteins / Q000302:isolation & purification* / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D003012:Microbial Collagenase / Q000037:antagonists & inhibitors* / Q000502:physiology; D011506:Proteins / Q000302:isolation & purification*; D012867:Skin / Q000201:enzymology; D014822:Vitreous Body / Q000032:analysis*
chemical_list: D043925:Angiogenesis Inducing Agents; D020533:Angiogenesis Inhibitors; D005136:Eye Proteins; D006023:Glycoproteins; D011506:Proteins; D003012:Microbial Collagenase