The chromosome-breaking activity of the restriction endonuclease Alu I in CHO cells is independent of the S-phase of the cell cycle.
The restriction endonuclease Alu I induces chromosomal aberrations in living Chinese hamster ovary (CHO) cells. Multiple fixation times reveal that the chromosome-breaking activity of Alu I is similar to that of ionizing radiation in that it is independent of the S-phase of the cell cycle. These results indicate that DNA double-strand breaks are the ultimate lesions for the production of chromosomal aberrations in all stages of the cell cycle.
D000818:Animals; D002453:Cell Cycle; D002460:Cell Line; D002869:Chromosome Aberrations; D002875:Chromosomes / Q000187:drug effects*; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000378:metabolism; D004262:DNA Restriction Enzymes / Q000378:metabolism / Q000494:pharmacology*; D015252:Deoxyribonucleases, Type II Site-Specific; D005260:Female; D005347:Fibroblasts / Q000187:drug effects; D007399:Interphase; D010053:Ovary
D004247:DNA; D004262:DNA Restriction Enzymes; C024669:endodeoxyribonuclease AluI; D015252:Deoxyribonucleases, Type II Site-Specific