A promoter for Bacillus subtilis expression vector.
A 117-bp EcoRI-PstI fragment with strong promoter activity (P1 promoter) was cloned from Bacillus subtilis chromosomal DNA and sequenced. The P1 promoter was shown to contain a putative -35 region (TTTACT) and -10 region (TAGATT), and promotes expression of cloned human interleukin-2 (IL-2) and human interferon-gamma (IFN-gamma) genes in B. subtilis.
D001412:Bacillus subtilis / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D005796:Genes; D005798:Genes, Bacterial; D005822:Genetic Vectors; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics*; D007376:Interleukin-2 / Q000235:genetics*; D010957:Plasmids; D011401:Promoter Regions, Genetic
D007376:Interleukin-2; D007371:Interferon-gamma; D004262:DNA Restriction Enzymes