title: Insertion of a long KpnI family member within a mitochondrial-DNA-like sequence present in the human nuclear genome.
abstract: Structural analysis of a phage lambda Charon 4A clone carrying one of the human nuclear mitochondrial(mut)-DNA-like sequences revealed that a KpnI-family member (KpnI 5.5-kb DNA) is inserted within this sequence. The inserted KpnI 5.5-kb DNA contains several possible polyadenylation signal sequences followed by an A-rich sequence at its 3' end and is flanked by perfect 13-bp direct repeats of the duplicated mtDNA-like sequences. These structures strongly suggest that the KpnI 5.5-kb DNA is a mobile element. Comparison of the 5' terminal sequences of the KpnI 5.5-kb DNA and four other long KpnI-family DNAs so far examined, using the predicted general promoter sequence for eukaryotic tRNAs, indicates that they contain the consensus sequences for the split internal RNA polymerase III control region.
mesh_terms: D001483:Base Sequence; D002467:Cell Nucleus / Q000032:analysis*; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D004251:DNA Transposable Elements; D004272:DNA, Mitochondrial / Q000235:genetics*; D015252:Deoxyribonucleases, Type II Site-Specific; D005260:Female; D005796:Genes; D006801:Humans; D009693:Nucleic Acid Hybridization; D010920:Placenta / Q000032:analysis; D010957:Plasmids; D011247:Pregnancy; D012091:Repetitive Sequences, Nucleic Acid
chemical_list: D004251:DNA Transposable Elements; D004272:DNA, Mitochondrial; D004262:DNA Restriction Enzymes; D015252:Deoxyribonucleases, Type II Site-Specific; C029312:GGTACC-specific type II deoxyribonucleases