Atrial natriuretic factor inhibits Na-K-Cl cotransport in teleost intestine.
Addition of atrial natriuretic factor (ANF) to the contraluminal side of the intestinal mucosa of a marine teleost, the winter flounder Pseudopleuronectes americanus, inhibits short-circuit current, net transepithelial fluxes of Na and Cl, and the unidirectional influx of Rb across the brush border membrane. This action of ANF is closely mimicked by addition of 8-bromo-guanosine 3',5'-cyclic monophosphate (8-BrcGMP). In contrast to the intestine, the opercular epithelium of the flounder did not respond to the in vitro addition of either ANF or 8-BrcGMP. Because intestinal salt and water absorption diminishes when marine fish enter water of lower salinity, ANF may be an important hormonal regulator through which euryhaline fish adapt to varying salinities.
D000818:Animals; D009320:Atrial Natriuretic Factor / Q000494:pharmacology*; D001692:Biological Transport; D002712:Chlorides / Q000378:metabolism*; D006152:Cyclic GMP / Q000031:analogs & derivatives / Q000494:pharmacology; D007413:Intestinal Mucosa / Q000378:metabolism*; D011188:Potassium / Q000378:metabolism*; D012964:Sodium / Q000378:metabolism*
D002712:Chlorides; C016276:8-bromocyclic GMP; D009320:Atrial Natriuretic Factor; D012964:Sodium; D006152:Cyclic GMP; D011188:Potassium