Determination of transferrin receptors on frozen sections of malignant B-cell lymphomas by immunofluorescence with a monoclonal antibody.
A series of 34 B-cell lymphomas was studied for the presence of transferrin receptors by immunofluorescence on frozen tissue sections with a monoclonal antibody. These lymphomas were classified by light microscopic study and furthermore investigated by immunohistochemical and morphometric methods. There were diffuse centrocytic, diffuse centroblastic, and follicular and diffuse centrocytic/centroblastic lymphomas. Significant differences in log mean nuclear area in these four histologic groups were found, as well as a significant correlation between log nuclear area and transferrin receptors as measured semiquantitatively by fluorescence intensity of the cell membranes. In addition, it was found that there is a strong correlation between the transferrin receptor activity and malignancy grading on histologic and morphometric basis. Therefore, the transferrin receptor determination on frozen sections appears to be a good method of malignancy grading.
D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal; D001402:B-Lymphocytes / Q000032:analysis / Q000473:pathology; D002462:Cell Membrane / Q000032:analysis; D005260:Female; D005455:Fluorescent Antibody Technique; D005629:Frozen Sections; D006801:Humans; D008223:Lymphoma / Q000032:analysis* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D011956:Receptors, Cell Surface / Q000032:analysis*; D011990:Receptors, Transferrin
D000911:Antibodies, Monoclonal; D011956:Receptors, Cell Surface; D011990:Receptors, Transferrin