Free radical-induced cross-linking of polydeoxythymidylic acid in deoxygenated aqueous solution.
Radiation-generated hydroxyl radicals and hydrogen atoms were shown to induce the cross-linking of polydeoxythymidylic acid (mol X wt approximately 170 000) in N2O-saturated acqueous solution. The irradiated samples were hydrolysed with formic acid and then analysed by high performance liquid chromatography. Products were isolated and subsequently characterized by capillary gas chromatography-mass spectrometry. The presence of previously described monomeric thymine products was also shown. Yields were determined and mechanisms of formation were described for the products.
D002851:Chromatography, High Pressure Liquid; D005609:Free Radicals; D008401:Gas Chromatography-Mass Spectrometry; D006859:Hydrogen; D006878:Hydroxides; D017665:Hydroxyl Radical; D011071:Poly T / Q000528:radiation effects*; D011089:Polydeoxyribonucleotides / Q000528:radiation effects*; D012996:Solutions; D014867:Water / Q000528:radiation effects
D005609:Free Radicals; D006878:Hydroxides; D011089:Polydeoxyribonucleotides; D012996:Solutions; D014867:Water; D011071:Poly T; D017665:Hydroxyl Radical; D006859:Hydrogen