Pulmonary neutrophil kinetics in sheep: effects of altered hemodynamics.
We investigated the effect of elevated left atrial pressure and reduced cardiac output on pulmonary neutrophil kinetics in the sheep. Sheep neutrophils were isolated, labeled with 111In-oxine, and reinfused. Erythrocytes were labeled with [99mTc]pertechnetate. A gamma camera measured the lung activities of the labeled neutrophils and erythrocytes. The results indicated that 38.5% of the total injected neutrophils marginated in the lung. Pulmonary hemodynamics were altered by inflating a left atrial balloon three times in each sheep for 15-30 min to achieve 5- to 25-mmHg increments in pulmonary arterial wedge pressure. At least a 30-min recovery period was allowed between inflations. After each left atrial balloon inflation, neutrophil uptake remained unchanged from base line, despite decreased mean cardiac output to 0.67 +/- 0.24 (+/- SD) 1/min and increased pulmonary blood volume. The absence of pulmonary neutrophil uptake was confirmed by arterial-venous measurements. Increased pulmonary blood volume had little effect on lung neutrophil uptake, suggesting that most of the pulmonary neutrophils are marginated. We conclude that the lungs have a large marginated neutrophil pool compared with the circulating pool and that reduced cardiac output and elevated left atrial pressure have no effect on pulmonary neutrophil kinetics in the sheep.
D000818:Animals; D001794:Blood Pressure; D001810:Blood Volume; D002302:Cardiac Output; D006439:Hemodynamics; D007700:Kinetics; D008168:Lung / Q000000981:diagnostic imaging; D009504:Neutrophils / Q000502:physiology*; D011652:Pulmonary Circulation; D011877:Radionuclide Imaging; D012756:Sheep; D013670:Sodium Pertechnetate Tc 99m
D013670:Sodium Pertechnetate Tc 99m