The association of ribulose-1,5-bisphosphate carboxylase with phosphoriboisomerase and phosphoribulokinase.
RuBPCase from peas showed Ribose-5-phosphate and Ribulose-5-phosphate dependent CO2 fixation when purified on sucrose gradients or by conventional methods. If purification was done in the presence of 20 mM MgCl2 and 20-25 mM NaHCO3 RuBPCase showed higher Ribose-5-phosphate and Ribulose-5-phosphate dependent CO2 fixation rates. Partially purified phosphoriboisomerase, phosphoribulokinase and RuBPCase from spinach could be reassociated in vitro.
D019747:Aldose-Ketose Isomerases; D002238:Carbohydrate Epimerases / Q000378:metabolism*; D002245:Carbon Dioxide / Q000378:metabolism; D002736:Chloroplasts / Q000201:enzymology; D004591:Electrophoresis, Polyacrylamide Gel; D007887:Fabaceae / Q000201:enzymology; D008854:Microscopy, Electron; D008970:Molecular Weight; D010770:Phosphotransferases / Q000378:metabolism*; D017853:Phosphotransferases (Alcohol Group Acceptor); D010946:Plants, Medicinal; D012273:Ribulose-Bisphosphate Carboxylase / Q000378:metabolism*
D002245:Carbon Dioxide; D010770:Phosphotransferases; D017853:Phosphotransferases (Alcohol Group Acceptor); C022390:phosphoribulokinase; D012273:Ribulose-Bisphosphate Carboxylase; D002238:Carbohydrate Epimerases; D019747:Aldose-Ketose Isomerases; C022548:ribosephosphate isomerase