Cloning and expression of an APH(3')-III phosphotransferase from Staphylococcus aureus in Streptomyces lividans.
An aminoglycoside 3' type III phosphotransferase derived from Staphylococcus aureus plasmid pRN1956 was cloned on the high copy number Streptomycetes vectors pIJ702 and pIJ704. Streptomyces lividans transformants carrying the hybrid plasmids show a resistance pattern towards aminoglycoside antibiotics comparable to the resistance pattern of S. aureus. The APH(3')-III with expanded spectrum of resistance, is a useful additional marker for gene cloning in Streptomycetes.
D003001:Cloning, Molecular; D004352:Drug Resistance, Microbial; D019868:Kanamycin Kinase; D010770:Phosphotransferases / Q000235:genetics*; D010957:Plasmids; D013211:Staphylococcus aureus / Q000201:enzymology / Q000235:genetics*; D013302:Streptomyces / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D014169:Transformation, Bacterial
D010770:Phosphotransferases; D019868:Kanamycin Kinase