Bovine alveolar macrophages: phenotypic and functional properties of subpopulations obtained by Percoll density gradient centrifugation.
Bronchoalveolar cells retrieved from conventionally raised, healthy calves were separated into four fractions on a discontinuous Percoll density gradient. The alveolar macrophage (AM) subpopulations and nonseparated AM were assayed for such phenotypic markers as la-antigen, ectoenzymes, and immune receptors, as well as for functional activity in antibody-dependent cellular cytotoxicity (ADCC) against virus-infected cells, superoxide anion generation, and their influence on lectin-induced lymphocyte proliferation. The low-density fraction was composed of large cells with low Ia-antigen expression, low ADCC activity, and high ecto-enzyme and C3b-receptor activity. In contrast the high-density fraction contained mainly small, monocytelike cells, with high Ia expression and low-level expression of most other markers and functions. Two fractions of intermediary density overlapped in most of the characteristics, but could be distinguished on the basis of ADCC activity, interleukin-1 generation, and the level of leucine amino peptidase activity.
D000818:Animals; D002417:Cattle; D002469:Cell Separation; D002499:Centrifugation, Density Gradient; D003602:Cytotoxicity, Immunologic; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D008213:Lymphocyte Activation; D008222:Lymphokines / Q000096:biosynthesis; D008264:Macrophages / Q000145:classification* / Q000201:enzymology / Q000276:immunology; D010587:Phagocytosis; D010641:Phenotype; D011205:Povidone; D011650:Pulmonary Alveoli / Q000166:cytology*; D011951:Receptors, Complement / Q000276:immunology; D017463:Receptors, Complement 3b; D011961:Receptors, Fc / Q000276:immunology; D012397:Rosette Formation; D012822:Silicon Dioxide
D000949:Histocompatibility Antigens Class II; D008222:Lymphokines; D011951:Receptors, Complement; D017463:Receptors, Complement 3b; D011961:Receptors, Fc; C016039:Percoll; D012822:Silicon Dioxide; D011205:Povidone