Monosomy 6 in a human lymphoma line induced by selection with a monoclonal antibody.
The human Epstein Barr Virus-superinfected B lymphoma cell line BJAB-B95.8.6 was mutagenized by gamma irradiation, and HLA mutants were selected with the HLA-Bw6-specific monoclonal antibody SFR8-B6. One of the mutants obtained, BM19, had lost one of the chromosomes 6 present in the wild type cells. Electrophoretic analysis of phosphoglucomutase isozyme PGM3 and erythrocyte glyoxalase 1 from both cells supports this conclusion. The HLA antigens expressed on BM19 were HLA-A2, B13, Bw4, C-, DR2 (questionable), DRw52 (weak) and DQw1. This constitutes one of the haplotypes of the wild type cells, the other (lost from BM19 cells) being HLA-A1, B35, Bw6, Cw4, DR5, DRw52 (strong) and DQw3. Possibilities to employ BM19 cells for the analysis of the major histocompatibility complex and other chromosome 6-encoded genes as well as their products are discussed.
D000911:Antibodies, Monoclonal; D002460:Cell Line; D002872:Chromosome Deletion; D002906:Chromosomes, Human, 6-12 and X; D008040:Genetic Linkage; D006680:HLA Antigens / Q000235:genetics* / Q000276:immunology; D006684:HLA-DR Antigens; D004854:Herpesvirus 4, Human; D000949:Histocompatibility Antigens Class II / Q000235:genetics*; D006801:Humans; D007621:Karyotyping; D008223:Lymphoma / Q000235:genetics; D009006:Monosomy
D000911:Antibodies, Monoclonal; D006680:HLA Antigens; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II