title: Intron mutations that affect the splicing efficiency of the CYH2 gene of Saccharomyces cerevisiae.
abstract: To define the extent of intervening sequences required for efficient splicing of the CYH2 gene in Saccharomyces cerevisiae, we have constructed a series of intron mutations. Artificial intron extensions of more than 300 bp of the natural intron lead to an inhibition of splicing whereas intron deletions lead to a drastic improvement of the splicing efficiency. It is shown that deletion of a 32 bp sequence element within the intron is responsible for this drastic improvement.
mesh_terms: D001483:Base Sequence; D004262:DNA Restriction Enzymes; D004251:DNA Transposable Elements; D005800:Genes, Fungal; D005822:Genetic Vectors; D009154:Mutation; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D012326:RNA Splicing; D012441:Saccharomyces cerevisiae / Q000235:genetics*
chemical_list: D004251:DNA Transposable Elements; D004262:DNA Restriction Enzymes