Distribution of a COOH-terminal amino acid extension of liver fructose-1,6-bisphosphatase among rodent species.
We have recently established from sequence analysis that rat liver fructose-1,6-bisphosphatase contains a 24-26 residue extension beyond the COOH-terminal amino acid of other mammalian fructose-1,6-bisphosphatases that results in an increased subunit molecular weight (Rittenhouse et al. (1983) J. Biol. Chem. 258, 7648-7652). In the present work the distribution of the COOH-terminal extension of fructose-1,6-bisphosphatases was tested by subunit molecular weight analysis of the enzyme immunoprecipitated from liver extracts. Of all rodent species tested, including several Muridae other than Rattus; only the enzyme from animals of the genus Rattus was found to have the extension. Further studies on the distribution of the enzyme extension could provide a simple tool to study the phylogeny of the genus Rattus.
D000595:Amino Acid Sequence; D000818:Animals; D003411:Arvicolinae / Q000378:metabolism; D006224:Cricetinae; D006597:Fructose-Bisphosphatase / Q000302:isolation & purification*; D005849:Gerbillinae / Q000378:metabolism; D008099:Liver / Q000201:enzymology*; D046911:Macromolecular Substances; D008647:Mesocricetus / Q000378:metabolism; D051379:Mice; D009115:Muridae / Q000378:metabolism; D051381:Rats; D012377:Rodentia / Q000378:metabolism*; D013045:Species Specificity
D046911:Macromolecular Substances; D006597:Fructose-Bisphosphatase