title: Phosphatidylcholine homeostasis in phosphatidylethanolamine-depleted Tetrahymena.
abstract: The relative contributions of the two pathways of phosphatidylcholine biosynthesis, phosphatidylethanolamine N-methyltransferase (EC 2.1.1.17) and diacylglycerol: CDP-choline cholinephosphotransferase (EC 2.7.8.1), are altered in the ciliate protozoan Tetrahymena thermophila whose phospholipid composition has been modified by culturing the organism in the presence of one of several aminophosphonic acids, as determined by measuring the incorporation of [methyl-3H]choline and [methyl-14C]methionine into phosphatidylcholine in vivo. In control cells the phosphotransferase pathway provides about 40% of the phosphatidylcholine, while in cells grown with 2-aminoethylphosphonate (AEP), 3-aminopropylphosphonate (APP), and N,N,N-trimethylaminoethyl-phosphonate (TMAEP) the contribution of the phosphotransferase pathway to phosphatidylcholine formation is 75, 90, and 26%, respectively. In AEP- and APP-grown cells, in which 80% of the phosphatidylethanolamine has been replaced by the corresponding phosphonolipid, the methyltransferase is less active since the level of the substrate phosphatidylethanolamine is reduced and neither of the phosphonolipids is a substrate for the enzyme. In TMAEP-grown cells, TMAEP competes with and reduces the incorporation of phosphocholine by the phosphotransferase pathway, leading to a smaller contribution of the pathway to phosphatidylcholine biosynthesis. The relative amounts of the two different radioactive labels incorporated into diacylphosphatidylcholine vs alkylacylphosphatidylcholine are also altered in the phosphonate-grown cells. The exogenous AEP induces a change in the glyceryl ether content of the 2-aminoethylphosphonolipid--33% in the AEP-grown cells compared to 70% in the control cells--indicating that the exogenous AEP is entering the phospholipids by the ethanolamine-phosphotransferase pathway rather than by the route of the endogenous AEP.
mesh_terms: D000615:Aminoethylphosphonic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D003470:Culture Media; D002798:Diacylglycerol Cholinephosphotransferase / Q000378:metabolism; D004987:Ethers / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D006706:Homeostasis; D008780:Methyltransferases / Q000378:metabolism; D010713:Phosphatidylcholines / Q000096:biosynthesis / Q000378:metabolism*; D050918:Phosphatidylethanolamine N-Methyltransferase; D010714:Phosphatidylethanolamines / Q000096:biosynthesis / Q000502:physiology*; D010743:Phospholipids / Q000378:metabolism; D011437:Propylamines / Q000494:pharmacology; D013768:Tetrahymena / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*
chemical_list: D003470:Culture Media; D004987:Ethers; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010743:Phospholipids; D011437:Propylamines; C034105:N,N,N-trimethyl-2-aminoethylphosphonate; D000615:Aminoethylphosphonic Acid; D008780:Methyltransferases; D050918:Phosphatidylethanolamine N-Methyltransferase; D002798:Diacylglycerol Cholinephosphotransferase; D005990:Glycerol; C011649:3-aminopropylphosphonic acid