Activation of rat c-raf during transfection of hepatocellular carcinoma DNA.
Rat c-raf was found to be activated in a transformant obtained with DNA of a hepatocellular carcinoma induced by 2-amino-3-methylimidazo[4,5-f]quinoline. This activated c-raf was cloned in a cosmid vector and actively transforming clones were obtained. Comparison of the restriction maps of this activated c-raf and cloned normal rat c-raf revealed a recombination in the 5'-terminal region of the activated form of this gene. The recombined DNA was shown to be actively transcribed and possibly to form a fused mRNA with c-raf, which is slightly smaller than normal c-raf mRNA. Since this recombination was not detected in the original tumor by Southern blot analysis, it presumably occurred during transfection.
D000818:Animals; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004273:DNA, Neoplasm / Q000235:genetics*; D005786:Gene Expression Regulation; D008114:Liver Neoplasms, Experimental / Q000235:genetics*; D011519:Proto-Oncogenes; D051381:Rats; D011995:Recombination, Genetic; D014158:Transcription, Genetic; D014162:Transfection; D014170:Transformation, Genetic
D004273:DNA, Neoplasm; D004262:DNA Restriction Enzymes