F'-coded, temperature-sensitive lambda cI857 repressor gene for easy construction and regulation of lambda promoter-dependent expression systems.
We describe the construction and properties of an F' factor which carries the temperature-sensitive cI857 allele of the repressor gene of coliphage lambda and which lacks the lambda cro function. This episome can easily be transferred to any F- and F' Escherichia coli strain, thus facilitating the construction and regulation of lambda promoter-dependent expression systems without the use of defective prophages.
D010582:Bacteriophage lambda / Q000235:genetics*; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005786:Gene Expression Regulation; D005809:Genes, Regulator; D005814:Genes, Viral; D010957:Plasmids; D012097:Repressor Proteins / Q000235:genetics*; D013696:Temperature; D014157:Transcription Factors / Q000235:genetics*
D012097:Repressor Proteins; D014157:Transcription Factors; D004262:DNA Restriction Enzymes