Presence of human papillomavirus type-16 and type-18 DNA sequences and their expression in cervical cancers and cell lines from Japanese patients.
Southern blot analyses of surgical specimens of cervical carcinoma from Japanese patients showed that 3/9 samples contained human papillomavirus (HPV) type-16 DNA sequences, and 2 contained HPV type-18 DNA sequences. By Northern blot analyses, RNA transcripts of HPV DNA sequences were demonstrated in some of the tissues containing HPV type-16 or HPV type-18 DNA sequences. Two cell lines established from cervical cancers of Japanese patients also contained HPV type-18 genomes and these cell lines contained HPV type-18 transcripts. Two other cervical cancer cell lines from a Japanese patient were found to contain HPV type-16 DNA sequences and their RNA transcripts.
D000230:Adenocarcinoma / Q000382:microbiology; D001483:Base Sequence; D002294:Carcinoma, Squamous Cell / Q000382:microbiology; D002460:Cell Line; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D005814:Genes, Viral; D006801:Humans; D027383:Papillomaviridae / Q000235:genetics*; D014158:Transcription, Genetic; D002583:Uterine Cervical Neoplasms / Q000209:etiology / Q000382:microbiology*
D004279:DNA, Viral