TSH-induced cyclic AMP production in an ovine thyroid cell line: OVNIS 5H.
The TSH-induced cyclic AMP response was studied using a 3-year-old ovine thyroid cell line TSH-independent for growth: OVNIS 5H. The kinetics of cyclic AMP production was followed both in cell layers and in cell culture media, with or without phosphodiesterase inhibitor. It is noteworthy that following the first wave in cyclic AMP obtained within minutes, we observed later a sustained exponential increase in cyclic AMP during the 5 days following TSH stimulation. A bioassay of TSH was derived allowing measurement of 1 microU/ml TSH from a crude bTSH preparation.
D000818:Animals; D002460:Cell Line; D002470:Cell Survival; D003470:Culture Media; D000242:Cyclic AMP / Q000096:biosynthesis*; D007700:Kinetics; D012756:Sheep; D013961:Thyroid Gland / Q000166:cytology / Q000378:metabolism* / Q000502:physiology; D013972:Thyrotropin / Q000494:pharmacology*
D003470:Culture Media; D013972:Thyrotropin; D000242:Cyclic AMP