title: Human ferritin light chain gene sequences mapped to several sorted chromosomes.
abstract: The iron storage ferritin light-chain gene exhibits multiple restriction enzyme fragments which have been mapped by analyzing sorted human chromosomes. A dual laser chromosome sorter was used to construct spot-blot filter panels representing 22 chromosome fractions. Hybridization of radiolabeled human ferritin-L gene probe to spot-blot panels revealed the ferritin-L gene on more than one chromosome. Miniaturized restriction enzyme analysis was used to map each of the ferritin-L restriction fragments uniquely to one of three chromosomes. This combination of sorted chromosome analyses provides a rapid method to map homologous DNA sequences located on more than one chromosome.
mesh_terms: D001483:Base Sequence; D002874:Chromosome Mapping; D002903:Chromosomes, Human, 19-20; D004247:DNA / Q000235:genetics; D004262:DNA Restriction Enzymes; D005293:Ferritins / Q000235:genetics*; D005434:Flow Cytometry; D005819:Genetic Markers; D006801:Humans
chemical_list: D005819:Genetic Markers; D004247:DNA; D005293:Ferritins; D004262:DNA Restriction Enzymes