c-Ha-ras not c-Ki-ras activation in three colon tumour cell lines.
Using the NIH 3T3 transformation assay system, an activated c-Ha-ras transforming gene has been identified in three distinct early passage colon carcinoma cell lines isolated from an invasive, differentiated, adenocarcinoma. The p21 c-Ha-ras gene product from these cell lines displayed an altered electrophorectic mobility and a point mutation in the DNA coding sequence leading to an amino acid substitution at position 12.
D000818:Animals; D002478:Cells, Cultured; D003110:Colonic Neoplasms / Q000235:genetics*; D004262:DNA Restriction Enzymes; D004273:DNA, Neoplasm / Q000235:genetics*; D005786:Gene Expression Regulation; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D011519:Proto-Oncogenes; D014170:Transformation, Genetic
D004273:DNA, Neoplasm; D004262:DNA Restriction Enzymes