title: Supercoil-dependent recognition of specific DNA sites by chromosomal protein HMG 2.
abstract: The ability of the chromosomal high mobility group protein HMG 2 to recognize supercoil-dependent structures within the chicken adult beta-globin gene was investigated by examining its ability to protect such sites from digestion by S1 nuclease. Low molar ratios of HMG 2 were found to be sufficient for complete inhibition of S1 cleavage of a supercoiled plasmid containing the globin gene. Furthermore, HMG 2 protected an S1 cleavage site within the 5'-flanking region of the globin gene, in preference to a palindromic S1 site within the plasmid vector.
mesh_terms: D000818:Animals; D001483:Base Sequence; D002645:Chickens; D004247:DNA / Q000097:blood*; D004262:DNA Restriction Enzymes; D004278:DNA, Superhelical / Q000097:blood*; D004720:Endonucleases; D004912:Erythrocytes / Q000378:metabolism*; D005796:Genes; D005914:Globins / Q000235:genetics*; D006609:High Mobility Group Proteins / Q000097:blood*; D011485:Protein Binding; D015719:Single-Strand Specific DNA and RNA Endonucleases
chemical_list: D004278:DNA, Superhelical; D006609:High Mobility Group Proteins; D005914:Globins; D004247:DNA; D004720:Endonucleases; D004262:DNA Restriction Enzymes; D015719:Single-Strand Specific DNA and RNA Endonucleases