title: The effect of cerulenin on the synthesis of the precursor gag polyprotein in defective murine leukemia and sarcoma virus producing cell lines.
abstract: The effect of cerulenin, an inhibitor of de novo fatty acid (and cholesterol) biosynthesis, on the synthesis of the precursor gag polyprotein, Pr65gag in a defective murine leukemia virus (334C) producing murine cell line (3JE) and a defective murine sarcoma virus (Gazdar) producing hamster cell line (HTG-2) was examined. In contrast to Moloney murine leukemia virus (M-MuLV) producing cell lines (MJD-54, clone 2) the amount of the Pr65gag remaining in the presence of cerulenin (20 micrograms/ml) was greatly reduced in both defective virus-infected cells. This effect appears specific for the Pr65gag polyprotein, since the env precursor polyprotein Pr80env was normally synthesized and remained undegraded in cerulenin-treated 3JE-infected cells. Thin-section electron micrographs showed an increased accumulation of virion particles in vesicles of treated HTG-2 cells.
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D002451:Cell Compartmentation; D002460:Cell Line; D002569:Cerulenin / Q000494:pharmacology*; D003673:Defective Viruses / Q000378:metabolism; D015683:Gene Products, gag; D009052:Leukemia Virus, Murine / Q000378:metabolism*; D008970:Molecular Weight; D011499:Protein Processing, Post-Translational / Q000187:drug effects*; D012191:Retroviridae Proteins / Q000096:biosynthesis*; D009053:Sarcoma Viruses, Murine / Q000378:metabolism*; D014759:Viral Envelope Proteins / Q000378:metabolism; D014779:Virus Replication
chemical_list: D000935:Antifungal Agents; D015683:Gene Products, gag; D012191:Retroviridae Proteins; D014759:Viral Envelope Proteins; D002569:Cerulenin