Altered growth properties and cell surface changes in ras transformed mouse bladder epithelium.
Transfection of the c-Ha-ras-1 oncogene, cloned from EJ/T24 cells, into different mouse bladder epithelial cell lines resulted in the acquisition of tumorigenic potential and, in all but one cell line (MB33I), anchorage-independent growth. Sera from syngeneic mice bearing tumours immunoprecipitated an 18 kDa protein from ras-transfected urothelial cells which was not detectable in their parental counterparts. Screening of a limited panel of mouse cell lines showed this protein to be urothelium-specific and associated with the expression of an activated ras gene. Polyoma middle T and v-myc-transfected bladder epithelial cells did not express this 18 kDa protein. Localization of this protein to the cell surface was demonstrated by immunofluorescence and absorption studies.
D000818:Animals; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000473:pathology*; D004847:Epithelial Cells; D005455:Fluorescent Antibody Technique; D051379:Mice; D008810:Mice, Inbred C57BL; D009857:Oncogenes; D011120:Polyomavirus / Q000235:genetics; D014176:Protein Biosynthesis; D014162:Transfection; D001743:Urinary Bladder / Q000166:cytology*