Nucleotide sequence of an heterochromatic segment recognized by the antibodies to Z-DNA in fixed metaphase chromosomes.
The purpose of this work was to analyse at the molecular level the DNA recognized by the antibodies to Z-DNA in in situ experiments. Antibodies to Z-DNA interact strongly with R-band positive heterochromatic segments of fixed metaphase chromosomes of Cebus (Viegas-Pequignot et al., 1983). These segments are constituted of a satellite DNA the repeat unit of which is about 1520 base pairs long. The base sequence of the repeat unit has been determined. It contains a (AC)n rich region which, in vitro, adopts the Z conformation under topological constraints. Experiments with nuclei suggest that this sequence is not predominantly in the Z conformation in vivo. The polymorphic structure of the (AC)n rich region argues for an active recombination sequence.
D000818:Animals; D000906:Antibodies; D000936:Antigen-Antibody Complex; D001482:Base Composition; D001483:Base Sequence; D002428:Cebus; D002875:Chromosomes / Q000648:ultrastructure*; D004247:DNA / Q000235:genetics*; D004262:DNA Restriction Enzymes; D004720:Endonucleases; D005260:Female; D006570:Heterochromatin / Q000032:analysis* / Q000648:ultrastructure; D008099:Liver / Q000648:ultrastructure; D008677:Metaphase; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D015719:Single-Strand Specific DNA and RNA Endonucleases
D000906:Antibodies; D000936:Antigen-Antibody Complex; D006570:Heterochromatin; D004247:DNA; D004720:Endonucleases; D004262:DNA Restriction Enzymes; D015719:Single-Strand Specific DNA and RNA Endonucleases