title: Differentiation of reference strains of leptospires of the Pomona serogroup by cross-agglutination absorption and restriction endonuclease analysis.
abstract: The serological classification of all reference strains that have been described as representing separate serovars of Leptospira interrogans within the Pomona serogroup was investigated using cross-agglutination absorption and bacterial restriction endonuclease analysis (BRENDA). Comparative cross-agglutination absorption studies indicated that cornelli CB, monjakov Monjakov and kennewicki LT1026 were homologous with pomona Pomona, and dania K1 and tsaratsova B81/7 were homologous with mozdok 5621. BRENDA confirmed these results, except that pomona Pomona and monjakov Monjakov showed a difference in the high molecular weight region. It is proposed that four serovars be currently recognised within the Pomona serogroup: pomona, mozdok, proechimys and tropica. The relative merits of the use of cross-agglutination absorption and BRENDA with respect to identification of Pomona serogroup isolates are discussed.
mesh_terms: D000372:Agglutination Tests; D000818:Animals; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000032:analysis*; D007919:Leptospira / Q000032:analysis / Q000145:classification* / Q000276:immunology; D011817:Rabbits
chemical_list: D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes