Influence of cholesterol feeding on the production of eicosanoids, tissue plasminogen activator and superoxide anion (O2-) by rabbit blood monocytes.
The metabolism in vitro of exogenous and endogenous arachidonic acid was studied in circulating blood monocytes obtained from control (control group) and cholesterol (0.5%)-fed (cholesterol group) rabbits. The production of superoxide anion (O2-), tissue plasminogen activator (t-PA) and adherence of monocytes were assessed in both groups of animals. The amounts of cyclooxygenase and lipoxygenase products derived from exogenously added [1-14C]AA were not significantly different in monocytes collected from both groups of animals. However, the amounts of PGD2, TXB2 and PGE2 formed from endogenous substrate were decreased significantly in monocytes obtained from the cholesterol group compared to those from the control group. The production of immunoreactive LTB4 was not suppressed significantly in monocytes collected from the cholesterol group. The production of O2- and t-PA was suppressed significantly in monocytes obtained from the cholesterol group and these cells adhered onto glass surfaces more efficiently than control cells. Since the formation of prostanoids from endogenous but not exogenous substrate is reduced, an effect of cholesterol on the liberation of AA from phospholipid pools is implicated.
D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000008:administration & dosage / Q000378:metabolism*; D002448:Cell Adhesion / Q000187:drug effects; D002791:Cholesterol, Dietary / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D009000:Monocytes / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D011817:Rabbits; D013481:Superoxides / Q000378:metabolism*; D010959:Tissue Plasminogen Activator / Q000378:metabolism*; D014280:Triglycerides / Q000097:blood
D001095:Arachidonic Acids; D002791:Cholesterol, Dietary; D014280:Triglycerides; D013481:Superoxides; D016718:Arachidonic Acid; D010959:Tissue Plasminogen Activator