Fluorescence polarization immunoassay to determine aminoglycoside modifying enzymes activity.
The possible use of polarized-immunofluorescence to evaluate aminoglycoside-modifying enzyme activities has been investigated. The TDX instrument was used to determine the residual quantity of aminoglycoside following inactivation during the assay procedure. Through the spectrum of the modified aminoglycoside antibiotics the enzymes encoded on eleven R-factors from clinical isolates of Enterobacteriaceae have been identified.
D000123:Acetyltransferases / Q000032:analysis; D000617:Aminoglycosides / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000378:metabolism*; D004755:Enterobacteriaceae / Q000201:enzymology* / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D005454:Fluorescence Polarization; D005455:Fluorescent Antibody Technique; D009713:Nucleotidyltransferases / Q000032:analysis; D010770:Phosphotransferases / Q000032:analysis; D011815:R Factors; D013379:Substrate Specificity; D014166:Transferases / Q000032:analysis*
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D014166:Transferases; D000123:Acetyltransferases; D010770:Phosphotransferases; D009713:Nucleotidyltransferases; C020658:glutamine-synthetase adenylyltransferase