5-lipoxygenase from rat PMN lysate.
The products of arachidonic acid metabolism in the 15,000xg supernatant of sonicated rat PMN are described. Only products derived from 5-lipoxygenase are observed. These products are 5-HETE and products derived from hydrolysis of LTA4, particularly LTB4. Some minor products derived from decomposition of 5-HPETE are also observed. The dependence of the activity of 5-lipoxygenase on enzyme and on substrate concentrations is presented and discussed in terms of a kinetic model that includes enzyme inactivation during turnover and substrate inhibition. The 5-lipoxygenase activity is stimulated by Ca++ and ATP.
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D001091:Arachidonate Lipoxygenases; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000097:blood; D002118:Calcium / Q000494:pharmacology; D002474:Cell-Free System; D002851:Chromatography, High Pressure Liquid; D004851:Epoxide Hydrolases / Q000097:blood; D006893:Hydroxyeicosatetraenoic Acids / Q000097:blood; D066298:In Vitro Techniques; D007700:Kinetics; D007975:Leukotriene B4 / Q000097:blood; D008084:Lipoxygenase / Q000097:blood*; D008297:Male; D009504:Neutrophils / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D001095:Arachidonic Acids; D006893:Hydroxyeicosatetraenoic Acids; D007975:Leukotriene B4; D016718:Arachidonic Acid; C022022:5-hydroxy-6,8,11,14-eicosatetraenoic acid; D000255:Adenosine Triphosphate; D001091:Arachidonate Lipoxygenases; D008084:Lipoxygenase; D004851:Epoxide Hydrolases; D002118:Calcium; C043134:leukotriene A4 hydrolase