Two unique restriction endonucleases from Neisseria lactamica.
Two new site-specific endonucleases, N1a III and N1a IV, have been isolated from Neisseria lactamica. N1a III recognizes the sequence, CATG, and cleaves 3' of the sequence to produce a four base 3' extension. N1a IV recognizes the sequence, GGNNCC, and cleaves between the two N's to produce blunt ended fragments.
D001483:Base Sequence; D002021:Buffers; D004262:DNA Restriction Enzymes / Q000302:isolation & purification* / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific; D009343:Neisseria / Q000201:enzymology*; D013379:Substrate Specificity
D002021:Buffers; D004262:DNA Restriction Enzymes; C048422:endodeoxyribonuclease NlaIII; C048423:endodeoxyribonuclease NlaIV; D015252:Deoxyribonucleases, Type II Site-Specific