Bovine opsin has more than one signal sequence.
By deletion of selected segments from a bovine opsin complementary DNA clone and subsequent analysis of transcripts in a cell-free translation-translocation system, we have localized two out of four theoretically conceivable signal sequences required for the integration of opsin into microsomal membranes.
D000595:Amino Acid Sequence; D000818:Animals; D001692:Biological Transport; D002417:Cattle; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D005136:Eye Proteins / Q000235:genetics* / Q000378:metabolism; D005818:Genetic Engineering; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics*; D008861:Microsomes / Q000378:metabolism; D014176:Protein Biosynthesis; D021382:Protein Sorting Signals / Q000235:genetics* / Q000378:metabolism; D012261:Ribonucleoproteins / Q000378:metabolism; D017299:Rod Opsins; D018271:Signal Recognition Particle; D013329:Structure-Activity Relationship
D005136:Eye Proteins; D006023:Glycoproteins; D008565:Membrane Proteins; D021382:Protein Sorting Signals; D012261:Ribonucleoproteins; D017299:Rod Opsins; D018271:Signal Recognition Particle; D004247:DNA