Affinity chromatographic procedure for the quantitative recovery of DNA fragments from agarose gels.
A simple and reliable method for the recovery of specific fragments of DNA from agarose gels is presented. The electroelution of the DNA onto the NENSORB cartridge matrix with the subsequent elution of the bound DNA by a methanol (50% v/v) wash has been shown to result in the quantitative recovery of the restriction fragment. Of importance is the fact that the DNA purified by this procedure is a viable substrate for further digestion by a second restriction endonuclease. The method does not require either phenol extraction or extensive desalting of the sample.
D010584:Bacteriophage phi X 174 / Q000235:genetics; D002846:Chromatography, Affinity / Q000379:methods; D004247:DNA / Q000302:isolation & purification*; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel
D004279:DNA, Viral; D004247:DNA; D004262:DNA Restriction Enzymes