An increase in prolyl-4-hydroxylase activity occurs during the retinoic acid-induced differentiation of mouse teratocarcinoma stem cell lines F9 and P19.
Monolayer cultures of F9 teratocarcinoma stem cells and P19 stem cells differentiate into endoderm, and fibroblast-like cells, respectively, when treated with retinoic acid. We demonstrate that this differentiation is associated with a large increase (greater than 40-fold) in the activity of an enzyme, prolyl-4-hydroxylase, involved in the posttranslational modification of collagens. This large increase in prolyl-4-hydroxylase activity occurs between 42 and 72 h after retinoic acid addition, and is associated with an increased amount of immunoprecipitable prolyl hydroxylase enzyme. This enzyme should be a useful marker for certain differentiated cell types produced during differentiation of teratocarcinoma stem cell lines.
D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D003094:Collagen / Q000378:metabolism; D004707:Endoderm / Q000473:pathology; D005347:Fibroblasts / Q000473:pathology; D007163:Immunosorbent Techniques; D007700:Kinetics; D051379:Mice; D011393:Procollagen-Proline Dioxygenase / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D013234:Stem Cells / Q000201:enzymology* / Q000473:pathology; D013724:Teratoma / Q000201:enzymology* / Q000473:pathology; D013806:Theophylline / Q000494:pharmacology; D014212:Tretinoin / Q000494:pharmacology*
D014212:Tretinoin; D003994:Bucladesine; D003094:Collagen; D013806:Theophylline; D011393:Procollagen-Proline Dioxygenase