Novel pathway for the metabolism of 6-trans-leukotriene B4 by human polymorphonuclear leukocytes.
Polymorphonuclear leukocytes  convert arachidonic acid to leukotriene B4 as well as to two 6-trans isomers of this substance. Both leukotriene B4 and 6-trans-leukotriene B4 are metabolized by a hydroxylase in human polymorphonuclear leukocytes to 20-hydroxy metabolites. We have now found a second, previously unknown, metabolic pathway for 6-trans-leukotriene B4 involving reduction of either the 6- or the 10- double bond. One of the two major metabolites of 6-trans-leukotriene B4 in human polymorphonuclear leukocytes is formed by the action of this reductase, followed by hydroxylation by leukotriene B4 20-hydroxylase. On the basis of ultraviolet (maximum absorbance at 232 nm) and mass spectral evidence, this product is either 5,12,20-trihydroxy-6,8,14-eicosatrienoic acid or 5,12,20-trihydroxy-8,10,14-eicosatrienoic acid.
D015126:8,11,14-Eicosatrienoic Acid / Q000031:analogs & derivatives / Q000097:blood; D002851:Chromatography, High Pressure Liquid; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007975:Leukotriene B4 / Q000097:blood*; D006899:Mixed Function Oxygenases / Q000097:blood; D009504:Neutrophils / Q000378:metabolism*; D010088:Oxidoreductases / Q000097:blood; D013056:Spectrophotometry, Ultraviolet
C048772:5,12,20-trihydroxy-6,8,14-eicosatrienoic acid; C048771:5,12,20-trihydroxy-8,10,14-eicosatrienoic acid; D007975:Leukotriene B4; D006899:Mixed Function Oxygenases; D010088:Oxidoreductases; D015126:8,11,14-Eicosatrienoic Acid