The effect of N-(2-carboxyphenyl)-4-chloroantranilic acid disodium salt (CCA) on concanavalin A-induced superoxide anion release in human mononuclear phagocytes.
Superoxide anion (O2-), generated and released by mononuclear phagocytes (M phi), plays significant roles in tissue damage accompanying inflammatory and immunological reactions. N-(2-carboxyphenyl)-4-chloroantranilic acid disodium salt (CCA), is a newly synthesized immunomodulator for which therapeutic efficacy in the treatment of rheumatoid arthritis (RA) has been reported. In this investigation, we have examined the effect of CCA on concanavalin A (Con A)-induced O2- release in human M phi. Exposure of M phi to Con A induced a large increase of O2- release. Preincubation of M phi with CCA suppressed O2- release induced by Con A almost to a control level. On the contrary, CCA itself did not have a significant effect on basal O2- release from M phi. These results indicate that the inhibition of O2- release from activated M phi may be an important factor in the anti-inflammatory effects of this drug.
D000893:Anti-Inflammatory Agents; D003208:Concanavalin A / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D008262:Macrophage Activation; D008264:Macrophages / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism*; D062367:ortho-Aminobenzoates / Q000494:pharmacology*
D000893:Anti-Inflammatory Agents; D062367:ortho-Aminobenzoates; D003208:Concanavalin A; D013481:Superoxides; C015735:lobenzarit