title: Double-strand cleavage at a two-base deletion mismatch in a DNA heteroduplex by nuclease S1.
abstract: A two-base deletion mismatch was generated in a DNA heteroduplex by hybridization of two linear plasmid DNA molecules differing only by the presence of a two-base deletion in one of them. The heteroduplex was shown to be sensitive to double-strand cleavage by nuclease S1, thus demonstrating the potential value of single-stranded probes for the detection of polymorphisms in genomic DNA due to very small deletions.
mesh_terms: D001483:Base Sequence; D002872:Chromosome Deletion; D004247:DNA / Q000235:genetics*; D004262:DNA Restriction Enzymes; D004720:Endonucleases / Q000378:metabolism*; D009692:Nucleic Acid Heteroduplexes / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D011815:R Factors; D015719:Single-Strand Specific DNA and RNA Endonucleases
chemical_list: D009692:Nucleic Acid Heteroduplexes; D004247:DNA; D004720:Endonucleases; D004262:DNA Restriction Enzymes; D015719:Single-Strand Specific DNA and RNA Endonucleases