title: Molecular cloning, DNA nucleotide sequencing, and expression in Bacillus subtilis cells of the Bacillus macerans cyclodextrin glucanotransferase gene.
abstract: The gene for cyclodextrin glucanotransferase from Bacillus macerans was cloned in an Escherichia coli bacteriophage, lambda D69, and was recloned in a Bacillus subtilis plasmid, pUB110. Starting from an ATG initiation codon, a unique reading frame was shown to extend for 2,142 base pairs (714 amino acids). The nucleotide sequence revealed that the enzyme is composed of two identical subunits.
mesh_terms: D000596:Amino Acids / Q000032:analysis; D001407:Bacillus / Q000235:genetics*; D001412:Bacillus subtilis / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes / Q000378:metabolism; D005786:Gene Expression Regulation; D005964:Glucosyltransferases / Q000235:genetics*; D008970:Molecular Weight
chemical_list: D000596:Amino Acids; D005964:Glucosyltransferases; C019963:cyclomaltodextrin glucanotransferase; D004262:DNA Restriction Enzymes