Manganese stimulates incorporation of [3H]inositol into an agonist-insensitive pool of phosphatidylinositol in brain membranes.
In a particulate preparation from rat brain, manganese ions stimulate the incorporation of [3H]inositol into inositol phospholipids in a concentration-dependent manner. Incubation with CDP-diacylglycerol (0.5 mM) alone had no effect on the incorporation of [3H]inositol but potentiated the stimulatory effect of manganese. Despite the increase in [3H]inositol incorporation into phosphatidylinositol, the carbachol-induced accumulation of [3H]inositol-1-phosphate was unaltered in membranes preincubated with manganese but when coincubated with CDP-diacylglycerol the carbachol-induced accumulation of [3H]inositol-1-phosphate was increased. These data suggest that manganese stimulates the incorporation of [3H]inositol into an agonist-insensitive pool of phosphatidylinositol.
D000818:Animals; D002217:Carbachol / Q000494:pharmacology; D002462:Cell Membrane / Q000378:metabolism; D003567:Cytidine Diphosphate Diglycerides / Q000494:pharmacology; D006624:Hippocampus / Q000378:metabolism*; D007294:Inositol / Q000378:metabolism*; D008297:Male; D008345:Manganese / Q000494:pharmacology*; D010716:Phosphatidylinositols / Q000096:biosynthesis*; D051381:Rats; D011919:Rats, Inbred Strains; D013268:Stimulation, Chemical
D003567:Cytidine Diphosphate Diglycerides; D010716:Phosphatidylinositols; D008345:Manganese; D007294:Inositol; D002217:Carbachol