Association of phosphorylated simian virus 40 T-antigen with subnuclear fractions of infected and transformed cells.
To define the roles of subnuclear structure in SV40 infection, the relative distribution of T-antigen (T-ag) in various subnuclear fractions obtained from both lytically infected and transformed African green monkey kidney cells was determined. Depending on the differential sensitivity of nuclear T-ag to extraction by salt and detergent, nuclear T-ag could be separated into nucleoplasmic T-ag, salt-sensitive T-ag and matrix-bound T-ag subclasses. At least fivefold less matrix-bound T-ag was found in transformed cells than in lytically infected cells. While a cAMP-independent protein kinase was detected in the nuclear matrix, the matrix-bound T-ag (94K) could not be phosphorylated in vitro. The removal of cellular chromosomes by DNase caused changes in the interaction of T-ag with nuclear components. The results suggest that the compartmentalization of nuclear T-ag may be determined by its interaction with host chromosomes.
D000818:Animals; D000957:Antigens, Viral, Tumor / Q000032:analysis*; D002213:Capsid / Q000032:analysis; D002458:Cell Fractionation; D002460:Cell Line; D002467:Cell Nucleus / Q000201:enzymology / Q000276:immunology*; D002472:Cell Transformation, Viral; D002522:Chlorocebus aethiops; D002875:Chromosomes / Q000502:physiology; D003593:Cytoplasm / Q000276:immunology; D004279:DNA, Viral / Q000096:biosynthesis; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism; D013539:Simian virus 40 / Q000032:analysis / Q000276:immunology* / Q000502:physiology; D014779:Virus Replication
D000957:Antigens, Viral, Tumor; D004279:DNA, Viral; D011494:Protein Kinases