title: Requirement of the Escherichia coli dnaA gene product for plasmid F maintenance.
abstract: There are DnaA protein-binding sites in at least one F origin of replication, and only potentially leaky dnaA(Ts) mutations had ever been used in previous studies indicating that F replication was independent of the dnaA gene product. Here we show that an Escherichia coli dnaA::Tn10 host which does not make a dnaA gene product cannot sustain autonomous or integrated F plasmid maintenance.
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology*; D004261:DNA Replication; D004926:Escherichia coli / Q000235:genetics* / Q000502:physiology; D005144:F Factor; D009154:Mutation
chemical_list: D001426:Bacterial Proteins