title: Partial characterization and solubilization of receptors for atrial natriuretic factor in rat glomeruli.
abstract: Specific receptors for atrial natriuretic factor (ANF) have been identified and solubilized in glomeruli from rat kidney. Radioiodinated synthetic ANF (Arg 101-Tyr 126) bound to a single class of high affinity (Kd 27 +/- 24 pM) sites with a density of 390 +/- 230 fmole/mg protein. The binding was time- and temperature-dependent, saturable and reversible. The ANF-receptor complex was not affected by angiotensin II, ACTH or vasopressin. Solubilization with 10 mM 3-[(3-cholamidopropyl)-dimethylammonio]- 1-propane sulfonate (CHAPS) slightly increased the affinity for ANF (Kd 5.0 +/- 3.3 pM) without affecting the density (250 +/- 110 fmole/mg protein). Similar results were found with 1% Triton X-100. ANF-related peptides interact generally in the same way with non-solubilized and solubilized receptors, indicating a fully preserved specificity of the receptors.
mesh_terms: D000324:Adrenocorticotropic Hormone / Q000494:pharmacology; D000804:Angiotensin II / Q000494:pharmacology; D000818:Animals; D009320:Atrial Natriuretic Factor / Q000378:metabolism; D002793:Cholic Acids; D003902:Detergents; D007678:Kidney Glomerulus / Q000032:analysis*; D008565:Membrane Proteins / Q000302:isolation & purification* / Q000378:metabolism; D017830:Octoxynol; D011092:Polyethylene Glycols; D051381:Rats; D017461:Receptors, Atrial Natriuretic Factor; D011956:Receptors, Cell Surface / Q000302:isolation & purification* / Q000378:metabolism; D012995:Solubility; D014667:Vasopressins / Q000494:pharmacology
chemical_list: D002793:Cholic Acids; D003902:Detergents; D008565:Membrane Proteins; D011956:Receptors, Cell Surface; C472610:atrial natriuretic peptide, rat; D014667:Vasopressins; D000804:Angiotensin II; D011092:Polyethylene Glycols; D009320:Atrial Natriuretic Factor; D000324:Adrenocorticotropic Hormone; D017830:Octoxynol; D017461:Receptors, Atrial Natriuretic Factor; C028213:3-((3-cholamidopropyl)dimethylammonium)-1-propanesulfonate