Molecular cloning of rat mitochondrial glutamic oxaloacetic transaminase mRNA and regulation of its expression in regenerating liver.
cDNA clones for rat mitochondrial glutamic oxaloacetic transaminase (mGOT) have been isolated from a rat liver cDNA library. One of the clones, designated p501, contained a cDNA insert of 1.4 kilobase pairs in length and hybridized to a mRNA of 2.4 kilobases from rat liver. We measured mGOT mRNA content in a regenerating rat liver. In a regenerating rat liver, mGOT activity was increased and reached maximum (170% of control activity) at about 48 h following the operation. Using the cDNA of mGOT, it was revealed that the increase of mGOT in the regenerating rat liver depended on its mRNA content.
D000818:Animals; D001219:Aspartate Aminotransferases / Q000235:genetics*; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D005786:Gene Expression Regulation; D007527:Isoenzymes / Q000235:genetics*; D008115:Liver Regeneration; D008297:Male; D008930:Mitochondria, Liver / Q000201:enzymology*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats
D007527:Isoenzymes; D012333:RNA, Messenger; D001219:Aspartate Aminotransferases; D004262:DNA Restriction Enzymes