title: Light and dark adaptation influences GABA receptor sites in the chick retina.
abstract: The aim of the present study was to investigate the effect of environmental conditions such as light-and-dark-adaptation on the plasticity of GABA receptor sites in the chick retina. In chicks exposed to light for 5 hr (light-adapted), specific [3H]GABA binding was increased by 35% in comparison to the binding found in chicks maintained in darkness (dark-adapted). Conversely, in the retina of chicks exposed to darkness for 5 hr, specific [3H]GABA binding was decreased by 28% with respect to that found in chicks kept in the light. Scatchard analysis of the binding data revealed that the affinity of GABA for its receptor binding site was higher in the retinas of light-adapted chicks than in those of dark-adapted chicks (Kd values of 19.20 +/- 1.23 and 27.20 +/- 1.47 nM, respectively). On the contrary, the maximal number of binding sites (Bmax) remained unchanged in light- and dark-adapted chicks (5.2 +/- 0.10 and 5.3 +/- 0.15 pmol/mg protein, respectively). These results suggest the involvement of GABA receptors in the regulation of visual function.
mesh_terms: D000818:Animals; D002645:Chickens / Q000502:physiology*; D003623:Dark Adaptation; D008566:Membranes / Q000378:metabolism; D009473:Neuronal Plasticity; D011963:Receptors, GABA-A / Q000378:metabolism*; D012160:Retina / Q000378:metabolism*; D014785:Vision, Ocular / Q000502:physiology
chemical_list: D011963:Receptors, GABA-A