Action of urokinase and trypsin on angiotensin, ACTH and the oxidized B chain of insulin.
N-terminal analysis of the products of hydrolysis of angiotensin, ACTH and the oxidized B chain of insulin after 4 h incubation with trypsin and urokinase reveals a great qualitative similarity in the action of the two enzymes. As expected, the rates of hydrolysis differ significantly and are much higher in the case of trypsin catalysis than in the case of urokinase catalysis. Unexpectedly, however, a decrease in the difference between the catalytic activity of the two enzymes, by increasing the number of Arg and Lys residues present in the substrate, has been observed.
D000324:Adrenocorticotropic Hormone / Q000378:metabolism*; D000804:Angiotensin II / Q000378:metabolism*; D006868:Hydrolysis; D007328:Insulin / Q000378:metabolism*; D007700:Kinetics; D010084:Oxidation-Reduction; D010446:Peptide Fragments / Q000378:metabolism; D014357:Trypsin / Q000378:metabolism*; D014568:Urokinase-Type Plasminogen Activator / Q000378:metabolism*
D007328:Insulin; D010446:Peptide Fragments; D000804:Angiotensin II; D000324:Adrenocorticotropic Hormone; D014357:Trypsin; D014568:Urokinase-Type Plasminogen Activator