Examination of the Rotazyme II enzyme immunoassay for the diagnosis of rotavirus gastroenteritis.
Rotazyme II, which is a shorter version of Rotazyme (less than 3 h), was compared with electron microscopy and Rotazyme for sensitivity and specificity on 229 human stool specimens. Compared with electron microscopy, the newer assay was found to be 99.4% sensitive and 97.3% specific for an overall agreement of 98.7%. After resolution of discordant results by blocking tests, the Rotazyme II enzyme immunoassay was shown to be more sensitive than electron microscopy and, therefore, 100% specific.
D000956:Antigens, Viral / Q000032:analysis*; D002648:Child; D002675:Child, Preschool; D005243:Feces / Q000382:microbiology; D005759:Gastroenteritis / Q000175:diagnosis* / Q000382:microbiology; D006801:Humans; D007124:Immunoenzyme Techniques; D007223:Infant; D008826:Microbial Sensitivity Tests; D008854:Microscopy, Electron; D011933:Reagent Kits, Diagnostic; D012401:Rotavirus / Q000276:immunology*; D012400:Rotavirus Infections / Q000175:diagnosis* / Q000382:microbiology
D000956:Antigens, Viral; D011933:Reagent Kits, Diagnostic