Elevation of Alu I-induced frequencies of chromosomal aberrations in Chinese hamster ovary cells by Neurospora crassa endonuclease and by ammonium sulfate.
The frequencies of chromosomal aberrations induced by the restriction endonuclease Alu I (recognition site AG/CT) can be elevated to a similar extent by additional treatments with a single-strand-specific endonuclease from Neurospora crassa (EC 3.1.30.1), or with ammonium sulfate in which the Neurospora endonuclease is suspended. These data indicate that Alu I does not produce DNA single-strand breaks in the chromatin of living cells, which can be recognized by the Neurospora endonuclease. The salt may induce conformational changes in the chromatin which make more recognition sites available for Alu I. Experiments with recovery times between the treatments with Alu I and the salt indicate that Alu I can act in the nucleus for at least 40 min.
D000645:Ammonium Sulfate / Q000494:pharmacology*; D000818:Animals; D002460:Cell Line; D002843:Chromatin / Q000648:ultrastructure; D002869:Chromosome Aberrations; D006224:Cricetinae; D004247:DNA / Q000378:metabolism; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D015252:Deoxyribonucleases, Type II Site-Specific; D004720:Endonucleases / Q000378:metabolism*; D005260:Female; D009492:Neurospora crassa / Q000201:enzymology; D009690:Nucleic Acid Conformation; D010053:Ovary; D015719:Single-Strand Specific DNA and RNA Endonucleases
D002843:Chromatin; D004247:DNA; D004720:Endonucleases; D004262:DNA Restriction Enzymes; C024669:endodeoxyribonuclease AluI; D015252:Deoxyribonucleases, Type II Site-Specific; D015719:Single-Strand Specific DNA and RNA Endonucleases; D000645:Ammonium Sulfate