title: Comparative effects of corticotropin-releasing factor, arginine vasopressin, and related neuropeptides on the secretion of ACTH and alpha-MSH by frog anterior pituitary cells and neurointermediate lobes in vitro.
abstract: The ability of corticoliberin (CRF), urotensin I, sauvagine, arginine-vasopressin (AVP), and mesotocin to stimulate ACTH release by frog anterior pituitary cells and alpha-melanotropin (MSH) by frog neurointermediate lobe was studied in vitro using a perifusion technique. CRF and AVP were found to be potent stimulators of ACTH secretion, whereas urotensin I and sauvagine were totally inactive. In opposition to recent findings in the rat. CRF did not modify alpha-MSH secretion by the frog neurointermediate lobe. Mesotocin, which is present in the parenchymal cells of the frog pars intermedia, had no effect on alpha-MSH release in vitro. No potentiation of CRF-induced ACTH release was observed when anterior pituitary cells were incubated with a combination of AVP and CRF. Together with the recent elucidation of a CRF-like molecule in the frog diencephalon, these results suggest that, in Amphibia, CRF and AVP exert their stimulatory action specifically on distal lobe corticotrophs.
mesh_terms: D000324:Adrenocorticotropic Hormone / Q000378:metabolism*; D029845:Amphibian Proteins; D000818:Animals; D001127:Arginine Vasopressin / Q000494:pharmacology; D003346:Corticotropin-Releasing Hormone / Q000494:pharmacology; D066298:In Vitro Techniques; D008297:Male; D009074:Melanocyte-Stimulating Hormones / Q000378:metabolism*; D010121:Oxytocin / Q000031:analogs & derivatives / Q000494:pharmacology; D036361:Peptide Hormones; D010455:Peptides / Q000494:pharmacology*; D010902:Pituitary Gland / Q000378:metabolism*; D011895:Rana ridibunda / Q000378:metabolism*; D011898:Ranidae / Q000378:metabolism*; D014579:Urotensins / Q000494:pharmacology
chemical_list: D029845:Amphibian Proteins; D036361:Peptide Hormones; D010455:Peptides; D014579:Urotensins; D001127:Arginine Vasopressin; C001713:mesotocin; D010121:Oxytocin; C025727:sauvagine; D000324:Adrenocorticotropic Hormone; D009074:Melanocyte-Stimulating Hormones; D003346:Corticotropin-Releasing Hormone; C029676:urotensin I