1,25-Dihydroxyvitamin D3 inhibits the clonogenic growth of transformed cells via its receptor.
Anchorage-independent growth in soft agar is a unique property of transformed cells which is known to be correlated with tumorigenicity. We report here that 1,25-dihydroxyvitamin D3 suppresses colony formation by a number of cultured cancer cell lines in soft agar in a dose dependent manner with an ID50 of 5-7 X 10(-10) M. This effect is also achieved with analogues of 1,25-dihydroxyvitamin D3 in accordance with their binding affinity for the hormone's receptor. Only cells with 1,25-dihydroxyvitamin D3 receptor protein are inhibited in their colony formation by vitamin D analogs indicating that the hormone receptor complex may be integrally involved in the in vitro suppression of the anchorage-independent phenotype.
D000970:Antineoplastic Agents / Q000494:pharmacology; D002117:Calcitriol / Q000494:pharmacology*; D002460:Cell Line; D004247:DNA / Q000378:metabolism; D014411:Neoplastic Stem Cells / Q000187:drug effects*; D018167:Receptors, Calcitriol; D011987:Receptors, Steroid / Q000032:analysis / Q000502:physiology*; D014807:Vitamin D / Q000494:pharmacology
D000970:Antineoplastic Agents; D018167:Receptors, Calcitriol; D011987:Receptors, Steroid; D014807:Vitamin D; D004247:DNA; D002117:Calcitriol