The effect of sulfhydryl compounds on the catalytic activity of Cu, Zn-superoxide dismutase purified from rat liver.
Sulfhydryl compounds such as reduced glutathione, cysteine and 2-mercaptopropionylglycine, a hepato-protective agent, activated Cu, Zn-superoxide dismutase purified from rat liver at low concentrations (below 10 microM). Furthermore we found evidence indicating that this activation is achieved by reducing Cu2+ present in the catalytic site of the dismutase, and thereby promoting the dismutation of superoxide anions.
D000818:Animals; D003300:Copper; D003545:Cysteine / Q000494:pharmacology; D004050:Ditiocarb / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D004793:Enzyme Reactivators / Q000494:pharmacology; D005978:Glutathione / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008099:Liver / Q000201:enzymology*; D010084:Oxidation-Reduction; D051381:Rats; D013438:Sulfhydryl Compounds / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D008625:Tiopronin / Q000494:pharmacology
D004793:Enzyme Reactivators; D013438:Sulfhydryl Compounds; D003300:Copper; D004050:Ditiocarb; D006861:Hydrogen Peroxide; D008625:Tiopronin; D013482:Superoxide Dismutase; D005978:Glutathione; D003545:Cysteine