title: Basolateral membrane properties of juxtamedullary proximal tubule in newborn rabbit.
abstract: We have evaluated the functional and morphological changes that occur in juxtamedullary proximal convoluted tubules before and after the completion of nephrogenesis at approximately 3 wk of age. Tubules were dissected in connection with glomeruli in the deep juxtamedullary zone, crimped at both ends, and induced to swell in isotonic medium by sequential exposure to ouabain, reduced bath protein concentration, and collagenase. Basolateral membrane surface areas were measured morphometrically in paired tubules if possible. The swelling rates and basolateral areas were the same in tubules from 2-7- and 14-17-day-old rabbits (during nephrogenesis), had increased in 28-42-day-old rabbits (after nephrogenesis), and increased further in adults. Because the surface areas and swelling rates increased proportionally, the nephrons "matured" only after nephrogenesis was complete and then only in basolateral surface area. The intrinsic membrane properties of hydraulic conductivity and ion permeability apparently remained constant.
mesh_terms: D000818:Animals; D000831:Animals, Newborn / Q000254:growth & development / Q000502:physiology*; D003470:Culture Media; D046508:Culture Techniques; D007679:Kidney Medulla; D007687:Kidney Tubules, Proximal / Q000033:anatomy & histology / Q000187:drug effects / Q000254:growth & development / Q000502:physiology*; D008566:Membranes / Q000502:physiology; D003012:Microbial Collagenase / Q000494:pharmacology; D010042:Ouabain / Q000494:pharmacology; D011817:Rabbits
chemical_list: D003470:Culture Media; D010042:Ouabain; D003012:Microbial Collagenase