The effect of plasma gelsolin on actin filaments. Ca2+-dependency of the capping and severing activities.
The Ca2+-dependency of plasma gelsolin capping and severing properties was investigated using viscometry, fluorescence of N-(1-pyrenyliodoacetamide)-labelled actin and Triton X-100 cell models. The two properties of plasma gelsolin appear to be expressed differently, severing being fully Ca2+-dependent, whereas capping seems to take place, even in the absence of Ca2+, although less efficiently.
D000199:Actins / Q000378:metabolism*; D000818:Animals; D002021:Buffers; D002118:Calcium / Q000502:physiology*; D002135:Calcium-Binding Proteins / Q000097:blood*; D002352:Carrier Proteins / Q000097:blood*; D002417:Cattle; D018260:Gelsolin; D008840:Microfilament Proteins / Q000097:blood*; D017830:Octoxynol; D011092:Polyethylene Glycols; D011817:Rabbits; D012995:Solubility; D013050:Spectrometry, Fluorescence; D014783:Viscosity
D000199:Actins; D002021:Buffers; D002135:Calcium-Binding Proteins; D002352:Carrier Proteins; D018260:Gelsolin; D008840:Microfilament Proteins; C016953:brevin; D011092:Polyethylene Glycols; D017830:Octoxynol; D002118:Calcium