NH3 and propionate modulate the morphological response of aggregation-competent Dictyostelium discoideum to cAMP.
Two metabolites, NH3 and propionic acid, are known to act as morphogens during the development of Dictyostelium discoideum, specifically altering the course of morphogenesis and cytodifferentiation. They have also been shown to modulate the cAMP relay in this organism: NH3 by restricting intracellular accumulation, and propionate by inhibiting extracellular release. In the present study, we utilized the light-scattering properties of aggregation-competent cells in agitated suspension to demonstrate that the morphological responses of such cells to exogenous cAMP are also modulated by NH3 and propionate in a manner that has interesting implications for the overall control of morphogenetic movements in D. discoideum. Our experiments were conducted using a newly designed continuous-flow apparatus that represents a significant improvement in the technique. The apparatus is described in detail.
D000643:Ammonium Chloride / Q000494:pharmacology*; D000242:Cyclic AMP / Q000494:pharmacology*; D004023:Dictyostelium / Q000166:cytology* / Q000187:drug effects; D007700:Kinetics; D008027:Light; D011422:Propionates / Q000494:pharmacology*; D012542:Scattering, Radiation
D011422:Propionates; D000643:Ammonium Chloride; D000242:Cyclic AMP; C029658:propionic acid