Altered cellular functions in a PC-12 cell clone chronically infected with retrovirus.
We characterized retrovirus-induced changes in PC-12 cell function and neuronal differentiation. PC-12 cells were infected with a neurotropic retrovirus (temperature-sensitive Moloney murine leukemia virus, mutant BA-1). We isolated a cell clone from this infected culture that displayed altered response to nerve growth factor; increased choline acetyltransferase activity; and decreased basal and nerve growth factor-stimulated acetylcholinesterase activity. In addition, Kirsten murine sarcoma virus infection of and subsequent expression of the v-ras oncogene in PC-12 cells induced neurite extension, enhanced choline acetyltransferase activity, and limited the growth potential of the infected cells.
D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D001369:Axons / Q000502:physiology; D002454:Cell Differentiation; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D002999:Clone Cells; D007708:Kirsten murine sarcoma virus; D007942:Leukemia, Experimental / Q000503:physiopathology; D008979:Moloney murine leukemia virus; D009414:Nerve Growth Factors / Q000502:physiology; D009474:Neurons / Q000201:enzymology / Q000502:physiology*; D012192:Retroviridae Infections / Q000201:enzymology / Q000503:physiopathology*; D012513:Sarcoma, Experimental / Q000503:physiopathology
D009414:Nerve Growth Factors; D002795:Choline O-Acetyltransferase; D000110:Acetylcholinesterase