Inhibition by ethanol of forskolin-stimulated adenylate cyclase in a murine neuroblastoma clone (N1E-115).
Forskolin, a diterpene activator of adenylate cyclase, stimulated the formation of cyclic AMP in intact murine neuroblastoma clone N1E-115 cells and stimulated adenylate cyclase activity in a membranal preparation from these cells. Ethanol caused a concentration-dependent inhibition of the forskolin-stimulated responses in both preparations. In intact cells, the inhibition appeared to be noncompetitive. However, in the membranal preparation the inhibition was more of a competitive nature. In addition, there was also a large difference in the amount of inhibition in the two systems. Thus, the inhibition by ethanol was nearly twice as much with intact cells as with membranes. Sucrose appeared to mimic these effects of ethanol, suggesting that with intact cells the effect of this alcohol may be due, in part, to changes in cellular osmotic pressure.
D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D002999:Clone Cells / Q000187:drug effects / Q000201:enzymology; D005576:Colforsin / Q000037:antagonists & inhibitors*; D000242:Cyclic AMP / Q000096:biosynthesis; D000431:Ethanol / Q000494:pharmacology*; D051379:Mice; D009447:Neuroblastoma / Q000201:enzymology*; D009997:Osmotic Pressure; D013395:Sucrose / Q000494:pharmacology; D014316:Tritium
D014316:Tritium; D005576:Colforsin; D000431:Ethanol; D013395:Sucrose; D000242:Cyclic AMP; D000262:Adenylyl Cyclases