title: Human cytomegalovirus virion-associated protein with kinase activity.
abstract: Protein kinase activity was detected in immunoprecipitates of human cytomegalovirus virions and infected cells by using a monoclonal antibody directed against an abundant 68,000-dalton virion structural protein. Purification of this protein by electrophoresis confirmed that the kinase activity was associated with this protein. The kinase activity was dependent on divalent cations (Mg2+, Mn2+) and cyclic nucleotide independent and exhibited optimal activity at pH 7 to 8. The kinase phosphorylated threonine and serine but not tyrosine.
mesh_terms: D000914:Antibodies, Viral / Q000276:immunology; D002412:Cations / Q000378:metabolism; D002478:Cells, Cultured; D003587:Cytomegalovirus / Q000201:enzymology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007158:Immunologic Techniques; D008970:Molecular Weight; D011494:Protein Kinases / Q000276:immunology / Q000378:metabolism*; D013379:Substrate Specificity; D014771:Virion / Q000201:enzymology
chemical_list: D000914:Antibodies, Viral; D002412:Cations; D011494:Protein Kinases