The regulatory chain in the p36-kd substrate complex of viral tyrosine-specific protein kinases is related in sequence to the S-100 protein of glial cells.
The major cytoplasmic target of various tyrosine-specific protein kinases is a 36-kd protein (p36). This protein can exist as a monomer or as a complex with a small subunit which seems to have a regulatory function. Amino acid sequence analysis of the small subunit from porcine intestine documents a unique polypeptide of 95 residues with a calculated mol. wt. close to 11 kd (p11). Since an immunologically related subunit of the same electrophoretic mobility is also found in the corresponding complex of chicken intestine p11 is well conserved across species. Unexpectedly, the sequence of p11 shows a high homology with the glia-specific protein S-100 whose biological function is not known. Although both proteins are dimers of rather small polypeptides we have not been able to detect in our preparations of p11 the moderate Ca2+ binding known for S-100. Certain implications of this sequence relation are discussed.
D000595:Amino Acid Sequence; D000818:Animals; D001358:Avian Sarcoma Viruses / Q000235:genetics*; D002417:Cattle; D002645:Chickens; D005809:Genes, Regulator; D007422:Intestines / Q000201:enzymology; D009457:Neuroglia / Q000378:metabolism; D010446:Peptide Fragments / Q000032:analysis; D011505:Protein-Tyrosine Kinases / Q000235:genetics*; D009418:S100 Proteins / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D013552:Swine; D014357:Trypsin
D010446:Peptide Fragments; D009418:S100 Proteins; D011505:Protein-Tyrosine Kinases; D014357:Trypsin