title: Cohabitation of scaffold binding regions with upstream/enhancer elements of three developmentally regulated genes of D. melanogaster.
abstract: We find DNA fragments attached to the nuclear scaffold (SARs) both 5' and 3' of three Drosophila genes, defining looped domains ranging from 4.5 to 13 kb. For the two-promoter-containing gene Adh (alcohol dehydrogenase), we find two upstream and two downstream SARs. For Sgs-4, the 5' SAR covers 866 bp immediately upstream of the transcript, and in the case of fushi tarazu, the 5' SAR is found on a small fragment 4.8 kb upstream of the start of transcription. These four upstream scaffold-attached fragments comap with enhancer-like regulatory sequences. Sequence analysis of five upstream SARs reveals clusters of sequences closely related to the cleavage consensus of topoisomerase II, several copies of a specific 10 bp A-rich sequence (AATAAATCAAA), and another 10 bp T-rich stretch.
mesh_terms: D000426:Alcohol Dehydrogenase; D000429:Alcohol Oxidoreductases / Q000235:genetics; D000818:Animals; D001665:Binding Sites; D002467:Cell Nucleus / Q000648:ultrastructure; D002843:Chromatin / Q000648:ultrastructure*; D004264:DNA Topoisomerases, Type I / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D004331:Drosophila melanogaster / Q000235:genetics* / Q000254:growth & development; D004742:Enhancer Elements, Genetic; D005796:Genes; D005809:Genes, Regulator; D007814:Larva; D009024:Morphogenesis; D011401:Promoter Regions, Genetic; D011506:Proteins / Q000235:genetics; D012471:Salivary Proteins and Peptides / Q000235:genetics
chemical_list: D002843:Chromatin; D004268:DNA-Binding Proteins; D011506:Proteins; D012471:Salivary Proteins and Peptides; D000429:Alcohol Oxidoreductases; D000426:Alcohol Dehydrogenase; D004264:DNA Topoisomerases, Type I