Susceptibility of different leukemic cell lines to the anticellular and antiviral effects of interferons.
A total of 18 different types of human leukemic cell lines were tested for their susceptibility to the anticellular and antiviral effects of interferons (IFNs) alpha, beta and gamma. In general, only the three myelogenous leukemic cell lines U937, KG-1 and HL-60 were found to be highly susceptible to the anticellular effect of the different IFNs while cells of the other lineages were relatively resistant. In order to determine whether the cell lines were sensitive to the antiviral effects of IFNs, the cells were first screened for their ability to support the replication of vesicular stomatitis virus (VSV), sindbis virus (SBV) and semliki forest virus (SFV). Unexpectedly, only three cell lines--Raji, K562 and U937 were highly susceptible to SFV while other cell lines were relatively refractory to all three viruses. Using SFV as indicator virus, the antiviral activity of all IFNs could be detected in all three cell lines and their relative efficiency was in the order of alpha greater than beta greater than gamma. The significance of these results were discussed.
D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002472:Cell Transformation, Viral; D004353:Drug Evaluation, Preclinical; D006801:Humans; D007370:Interferon Type I / Q000633:toxicity*; D007371:Interferon-gamma / Q000633:toxicity*; D007938:Leukemia / Q000473:pathology*; D012672:Semliki forest virus / Q000235:genetics*; D012845:Sindbis Virus / Q000235:genetics*; D014721:Vesicular stomatitis Indiana virus / Q000235:genetics*
D007370:Interferon Type I; D007371:Interferon-gamma