NAD+ glycohydrolase of the plasma membrane prepared from glial and neuronal cells.
NAD+ glycohydrolase (EC 3.2.2.5) activity was detected in the plasma membrane prepared from the primary culture of rat astrocytes. The enzyme has a broad optimum pH range. From the kinetic analysis, a Michaelis constant of 91.2 microM and a maximum velocity of 0.785 mumol/min/mg protein were obtained. ADPribose exhibited a competitive inhibition with respect to NAD. The inhibition by nicotinamide was shown to be of a non-competitive type. ATP and GTP were found to be competitive inhibitors. NAD+ glycohydrolase activity was not detected in the plasma membrane prepared from the primary culture of neuronal cells of chick embryos.
D000246:Adenosine Diphosphate Ribose / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D001667:Binding, Competitive; D001921:Brain / Q000201:enzymology; D002462:Cell Membrane / Q000201:enzymology; D002478:Cells, Cultured; D002642:Chick Embryo; D006160:Guanosine Triphosphate / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009243:NAD / Q000378:metabolism; D009244:NAD+ Nucleosidase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D009457:Neuroglia / Q000201:enzymology*; D009474:Neurons / Q000201:enzymology*; D009536:Niacinamide / Q000378:metabolism / Q000494:pharmacology; D051381:Rats
D009243:NAD; D000246:Adenosine Diphosphate Ribose; D009536:Niacinamide; D006160:Guanosine Triphosphate; D000255:Adenosine Triphosphate; D009244:NAD+ Nucleosidase