A silica-based reversed-phase column for single-stranded oligodeoxyribonucleotides.
A novel, silica-based, reversed-phase column targeted to the separation of single-stranded oligodeoxyribonucleotides has been developed by grafting monochlorooctadecylsilane onto silica which is a base material of reversed-phase columns (TSK 120A and 120T), in which polychlorosilane is used as a grafting reagent. By applying a shallow gradient elution of an aqueous acetonitrile solution containing 0.1 M ammonium acetate, chromatography with this column produced well-resolved peaks for large samples such as those having bases up to 26.
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides / Q000302:isolation & purification*; D012822:Silicon Dioxide
D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides; D012822:Silicon Dioxide