Mitochondrial location of rat liver dinucleoside triphosphatase.
Rat liver dinucleoside triphosphatase (EC 3.6.1.29) is associated with sucrose-gradient purified mitochondria and can be extracted by freeze and thaw treatment. The proportion of mitochondrial dinucleoside triphosphatase approaches 50% of total liver enzyme. Evidence is also presented that 10% of total liver bis(5'-guanosyl)tetraphosphatase (EC 3.6.1.17) might be equally linked to mitochondria. Those data suggest that diadenosine 5',5'''-P1,P3-triphosphate, diadenosine 5',5'''-P1,P4-tetraphosphate, or other substrates of those enzymes, might be somehow related to mitochondria or mitochondrial function(s), although the occurrence of dinucleoside polyphosphates has not been reported in that organelle.
D017766:Acid Anhydride Hydrolases; D000818:Animals; D002499:Centrifugation, Density Gradient; D002850:Chromatography, Gel; D015226:Dinucleoside Phosphates; D005260:Female; D005615:Freezing; D008099:Liver / Q000201:enzymology; D008930:Mitochondria, Liver / Q000201:enzymology*; D009841:Oligonucleotides / Q000378:metabolism; D010744:Phosphoric Monoester Hydrolases / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism; D011092:Polyethylene Glycols / Q000008:administration & dosage; D051381:Rats; D000099170:Bis(5'-adenosyl)triphosphatase
D017766:Acid Anhydride Hydrolases; D015226:Dinucleoside Phosphates; D009841:Oligonucleotides; D010744:Phosphoric Monoester Hydrolases; D011092:Polyethylene Glycols; D000099170:Bis(5'-adenosyl)triphosphatase; C020023:bis(5'-nucleosyl)tetraphosphatase (asymmetrical); C016811:tyloxapol