title: Analysis of the mouse dhfr promoter region: existence of a divergently transcribed gene.
abstract: The use of murine dihydrofolate reductase (dhfr) gene amplification mutants enabled us to identify important structural and functional features of the dhfr promoter region. We found another transcription unit, at least 14 kilobases in size, which initiates within 130 base pairs of the major dhfr transcript and is transcribed divergently. The 5' ends of both transcripts were analyzed and found to have multiple initiation sites. The major dhfr transcript and the divergent transcript appear to share the same promoter region; the longer transcripts of the dhfr gene overlap with the divergent transcripts and use a different promoter region. The divergent transcript appears to code for a protein; an homologous sequence to its first exon is found in the corresponding location near the human dhfr gene.
mesh_terms: D000546:Amanitins / Q000494:pharmacology; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004307:Dose-Response Relationship, Radiation; D004720:Endonucleases; D005784:Gene Amplification; D005796:Genes / Q000187:drug effects; D051379:Mice; D009154:Mutation; D010442:Peptide Chain Initiation, Translational; D011401:Promoter Regions, Genetic; D015719:Single-Strand Specific DNA and RNA Endonucleases; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics*; D014158:Transcription, Genetic / Q000187:drug effects / Q000528:radiation effects; D014466:Ultraviolet Rays
chemical_list: D000546:Amanitins; D013762:Tetrahydrofolate Dehydrogenase; D004720:Endonucleases; D015719:Single-Strand Specific DNA and RNA Endonucleases