A note on the use of protease inhibitors during chromatin fractionation on hydroxyapatite columns.
It was found that NaHSO3 present in the eluents enabled full and reproducible recovery of chromosomal proteins from a hydroxyapatite column. Another protease inhibitor, PMSF, did not have that effect.
D000818:Animals; D002458:Cell Fractionation / Q000379:methods; D002843:Chromatin / Q000302:isolation & purification* / Q000648:ultrastructure; D002845:Chromatography / Q000379:methods; D017886:Durapatite; D006882:Hydroxyapatites; D008099:Liver / Q000648:ultrastructure; D008297:Male; D010664:Phenylmethylsulfonyl Fluoride; D051381:Rats; D011919:Rats, Inbred Strains; D013450:Sulfones
D002843:Chromatin; D006882:Hydroxyapatites; D013450:Sulfones; D010664:Phenylmethylsulfonyl Fluoride; D017886:Durapatite