3' c-myc rearrangement in a human leukemic T-cell line.
The human leukemic T-cell line Hut 78, derived from a patient suffering from Sézary syndrome and expressing a mature postthymic membrane phenotype, shows a c-myc rearrangement beginning within 500 base pairs immediately 3' to the c-myc exon 3. Chromosome analysis of the Hut 78 reveals the presence of a hyperdiploid karyotype with a large number of markers and rearrangements, though trisomy is the only cytogenetic anomaly involving chromosome 8. Moreover, as the abnormal c-myc appears to be duplicated, a duplication of the chromosome 8 carrying the abnormal c-myc probably occurred. Unlike four other human leukemic T-cell lines tested, the Hut 78 cells express a high amount of c-myc transcript, suggesting that the 3' c-myc anomaly may cause a deregulation of the expression of this gene.
D001483:Base Sequence; D002460:Cell Line; D004262:DNA Restriction Enzymes; D004273:DNA, Neoplasm / Q000235:genetics*; D005786:Gene Expression Regulation; D005796:Genes; D006801:Humans; D007621:Karyotyping; D011519:Proto-Oncogenes; D012751:Sezary Syndrome / Q000235:genetics*; D013601:T-Lymphocytes / Q000502:physiology*
D004273:DNA, Neoplasm; D004262:DNA Restriction Enzymes