Modulation of cytosolic protein kinase C activity by ferricyanide: priming event seems transmembrane redox signalling. A study on transformed C3H/10T1/2 cells in culture.
Transformed 3T3/10T1/2 cultured cells incubated with ferricyanide caused a decrease of 2 mM EDTA extractable cytosolic protein kinase C activity in 2 min, whereas 5 or 20 min ferricyanide treatment reverted the enzyme activity to that observed without ferricyanide. The ferricyanide effect in 2 min was abolished by amiloride and sustained by ouabain. Thus, deactivation-activation of cytosolic protein kinase C is attributed to an unknown signal generation during H+ accumulation coupled with the Na+/H+ exchange phase. In this mechanism the priming event concerns the transmembrane redox process shedding H+ into the cell interior while impermeant ferricyanide acts as a unique electron acceptor.
D000818:Animals; D017920:Antiporters; D002352:Carrier Proteins / Q000032:analysis; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D003600:Cytosol / Q000201:enzymology*; D004492:Edetic Acid / Q000494:pharmacology; D005292:Ferricyanides / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D010042:Ouabain / Q000494:pharmacology; D010084:Oxidation-Reduction; D011188:Potassium / Q000378:metabolism; D011493:Protein Kinase C / Q000378:metabolism*; D011522:Protons; D012964:Sodium / Q000378:metabolism
D017920:Antiporters; D002352:Carrier Proteins; D005292:Ferricyanides; D011522:Protons; C007931:hexacyanoferrate III; D010042:Ouabain; D004492:Edetic Acid; D012964:Sodium; D011493:Protein Kinase C; D011188:Potassium