Ontogeny of pepsin secretory response to secretagogues in isolated rat gastric glands.
By use of isolated gastric glands from rats at various ages, we demonstrated that full-term neonate and 1-day-old rats showed no response to cholecystokinin octapeptide (CCK-OP), carbachol, or Ca2+ ionophore. The same glands, however, were responsive to dibutyryl cAMP. A mature response was not found until the pups were 2 days old. Injection of hydrocortisone into newborn rats led to an increase in pepsinogen concentrations in gastric glands and also an increased responsiveness to CCK-OP, carbachol, and Ca2+ ionophore A23187 24 h after administration. Hydrocortisone thus caused precocious maturation of both pepsinogen accumulation and pepsinogen secretory responsiveness of gastric glands in rat pups.
D000818:Animals; D000833:Animals, Suckling / Q000378:metabolism; D003994:Bucladesine / Q000494:pharmacology; D000001:Calcimycin / Q000494:pharmacology; D002217:Carbachol / Q000494:pharmacology; D005753:Gastric Mucosa / Q000201:enzymology / Q000378:metabolism*; D006854:Hydrocortisone / Q000494:pharmacology; D066298:In Vitro Techniques; D007552:Isotonic Solutions / Q000494:pharmacology; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D010434:Pepsin A / Q000378:metabolism*; D010435:Pepsinogens / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D000077325:Ringer's Lactate; D012844:Sincalide / Q000494:pharmacology; D013268:Stimulation, Chemical
D007552:Isotonic Solutions; D010435:Pepsinogens; D000077325:Ringer's Lactate; D000001:Calcimycin; D003994:Bucladesine; D002217:Carbachol; D007770:L-Lactate Dehydrogenase; D010434:Pepsin A; D012844:Sincalide; D006854:Hydrocortisone