title: Mechanism of phagocytosis of mycobacteria by Schwann cells and their comparison with macrophages.
abstract: Factors influencing the phagocytosis of mycobacteria by 33B rat Schwannoma cells and rat peritoneal macrophages were studied. Uptake of 14C-acetate-labeled Mycobacterium w by these cells was used to set up a radiometric phagocytosis assay. Incubation at 4 degrees C and treatment with sodium azide (0.2% to 1%), colchicine (10(-7) to 10(-3) M), cytochalasin B (0.2 micrograms/ml to 25 micrograms/ml), and dibutyryl cyclic AMP (10(-7) to 10(-3) M) inhibited the phagocytosis by both cell types in a similar manner. These experiments demonstrate similarities in the mechanism of phagocytosis of mycobacteria by Schwann cells and macrophages.
mesh_terms: D000818:Animals; D001386:Azides / Q000494:pharmacology; D003994:Bucladesine / Q000494:pharmacology; D002478:Cells, Cultured; D003078:Colchicine / Q000494:pharmacology; D003080:Cold Temperature; D003571:Cytochalasin B / Q000494:pharmacology; D008264:Macrophages / Q000502:physiology*; D009161:Mycobacterium / Q000502:physiology*; D009442:Neurilemmoma; D010587:Phagocytosis / Q000187:drug effects; D051381:Rats; D012583:Schwann Cells / Q000502:physiology*; D019810:Sodium Azide
chemical_list: D001386:Azides; D003571:Cytochalasin B; D003994:Bucladesine; D019810:Sodium Azide; D003078:Colchicine