[Transfer of active oncogenes and promoters into the mouse cell genome].
Different cell DNA's (normal NIH 3T3 DNA; human osteosarcoma cell DNA; human malignant glioma cell DNA with amplified c-Ha-ras) were cotransfected onto NIH 3T3 cells with cloned long terminal repeat (LTR) sequences of Rous sarcoma virus. LTR RSV and normal NIH 3T3 DNA c-fos oncogen expression was detected in tumors induced in nude mice. In the same system human tumour cell DNA with amplified c-Ha-ras gene was used, that to the integration and amplification of LTP sequences with simultaneous maintenance of c-Ha-ras amplification. Nude mouse tumour DNA with integrated LTR sequences was active in successive rounds of transfection.
D000818:Animals; D001358:Avian Sarcoma Viruses / Q000235:genetics*; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002472:Cell Transformation, Viral; D005814:Genes, Viral; D005822:Genetic Vectors; D006801:Humans; D051379:Mice; D009368:Neoplasm Transplantation; D011401:Promoter Regions, Genetic; D011519:Proto-Oncogenes; D012091:Repetitive Sequences, Nucleic Acid; D014162:Transfection
Perenos aktivnykh onkogenov i promotorov v genom kletok mysheĭ.