Direct detection of the human papovavirus BK in urine of bone marrow transplant recipients: comparison of DNA hybridization with ELISA.
Urine specimens from bone marrow transplant (BMT) recipients and from controls were directly tested for BK virus (BKV) DNA sequences by dot hybridization and for BKV antigen by a double-antibody indirect ELISA. A total of 158 specimens from 55 BMT patients (57 collected prior to or at the time of transplantation and 101 in the posttransplant period) and single urines from 125 control subjects were examined by both methods. A molecularly cloned, 32P-labelled BKV probe was hybridized with urine sediments that were spotted directly on nitrocellulose filters and denatured in situ. BKV DNA sequences were detected in 1 (1.8%) pretransplant and 22 (21.8%) posttransplant urines of BMT patients, and in none of control urines. In ELISA of urine supernatants, BKV antigen was detected in 1 (1.8%) pretransplant and 21 (20.8%) posttransplant urines of BMT patients and in 1 (0.8%) of the control urines. The results of the two tests correlated as follows: 16 urines were positive and 253 urines negative by both methods; seven specimens were positive by DNA hybridization only and seven were positive by ELISA alone. Virus excretion in urine was demonstrated in 20 (36.4%) patients by DNA hybridization, in 19 (34.5%) patients by ELISA, in 15 (27.3%) patients by both methods, and in 24 (44%) patients by at least one of the two tests.
D000293:Adolescent; D000328:Adult; D000956:Antigens, Viral / Q000032:analysis; D001739:BK Virus / Q000032:analysis / Q000235:genetics / Q000276:immunology; D001483:Base Sequence; D016026:Bone Marrow Transplantation; D002648:Child; D002675:Child, Preschool; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D005814:Genes, Viral; D006801:Humans; D007223:Infant; D009693:Nucleic Acid Hybridization; D011120:Polyomavirus / Q000032:analysis / Q000235:genetics / Q000276:immunology; D014556:Urine / Q000382:microbiology*
D000956:Antigens, Viral; D004279:DNA, Viral