Augmentation of the neutralisation test for type 1 HSV: evidence of high representation of neutralising antibody in the adult community.
Optimal neutralisation of type 1 herpes simplex virus was obtained by reacting undiluted human serum with virus for 4 h at 37 degrees C, followed by addition of antihuman globulin for 20 min; under these conditions it was possible to detect neutralising antibody activity in 40 of 45 human sera (88%) previously adjudged to be negative by conventional neutralisation tests.
D000888:Antibodies, Anti-Idiotypic / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology*; D003165:Complement System Proteins / Q000276:immunology; D004306:Dose-Response Relationship, Immunologic; D006561:Herpes Simplex / Q000276:immunology*; D006801:Humans; D009500:Neutralization Tests; D018139:Simplexvirus / Q000276:immunology*; D013696:Temperature; D013997:Time Factors
D000888:Antibodies, Anti-Idiotypic; D000914:Antibodies, Viral; D003165:Complement System Proteins