The superoxide dismutase activity of myeloperoxidase; formation of compound III.
The reaction of superoxide anions with myeloperoxidase (donor: hydrogen-peroxide oxidoreductase, EC 1.11.1.7), which results in the formation of Compound III of myeloperoxidase, was investigated. It is shown that myeloperoxidase has a high affinity for superoxide anions because formation of Compound III was only partially inhibited by high concentrations of superoxide dismutase. Furthermore, when superoxide anions were generated in a mixture of both cytochrome c and myeloperoxidase in the absence of Cl-, only Compound III was formed and reduction of cytochrome c was not observed. In the presence of Cl-, Compound III was also formed and reduction of cytochrome c was inhibited. From the results described in this paper we conclude that Compound III is able to react with superoxide anions, probably resulting in formation of an intermediate (Compound I) which is catalytically active in the oxidation of Cl- to yield hypochlorous acid (HOCl). Because Compound III of myeloperoxidase is formed in phagocytosing neutrophils (Winterbourn, C.C., Garcia, R.C. and Segal, A.W. (1985) Biochem. J. 228, 583-592) we propose that, in vivo, myeloperoxidase also acts as a superoxide dismutase, and via formation of Compound I uses superoxide anions in the formation of HOCl.
D002374:Catalase / Q000378:metabolism; D003574:Cytochrome c Group / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D007962:Leukocytes / Q000201:enzymology; D009195:Peroxidase / Q000097:blood*; D013482:Superoxide Dismutase / Q000097:blood*; D014969:Xanthine Oxidase / Q000378:metabolism
D003574:Cytochrome c Group; D002374:Catalase; D009195:Peroxidase; D013482:Superoxide Dismutase; D014969:Xanthine Oxidase