Replication of varicella zoster virus in Raji cells.
This report provides evidence for the replication of varicella zoster virus (VZV) in Raji cells. Infection was achieved by co-cultivation of Raji cells with VZV-infected human fibroblasts. Replication of VZV, as assessed by immunofluorescence using monoclonal antibodies against VZV-glycoproteins, ranged from 18 to 24% of the cells. Electron microscopy detected complete virions within the membrane-bound cytoplasmic vesicles and free viral particles in the nuclear matrix as late as 12 days post-infection. Western blot analysis of infected Raji cells demonstrated VZV-specific glycoproteins. The availability of a VZV-susceptible cell line growing in suspension culture provides a useful model for future studies.
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D002460:Cell Line; D005455:Fluorescent Antibody Technique; D006023:Glycoproteins / Q000276:immunology; D014645:Herpesvirus 3, Human / Q000254:growth & development* / Q000276:immunology / Q000648:ultrastructure; D006801:Humans; D007163:Immunosorbent Techniques; D008854:Microscopy, Electron; D014771:Virion / Q000648:ultrastructure
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D006023:Glycoproteins