The nucleotide sequence and the tissue-specific expression of Drosophila c-src.
We have examined the coding capability and expression of the Drosophila homolog of the vertebrate proto-oncogene c-src. Sequence analysis of a cDNA clone representing the Drosophila c-src locus suggests that the gene encodes a 62 kd protein that is remarkably similar to the protein product of chicken c-src. The Drosophila c-src locus is transcribed into three mRNAs that are each regulated independently during development. Drosophila c-src RNA is abundant in embryos and pupae but rare in larvae and adults. In situ hybridization reveals that after the first 8 hr of development, c-src RNA accumulates almost exclusively in neural tissues such as the brain, ventral nerve chord, and eye-antennal discs, and in differentiating smooth muscle. We conclude that c-src may not be a mitotic signal but instead may play a role in the development of neural tissue and smooth muscle.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004330:Drosophila / Q000166:cytology / Q000235:genetics*; D029721:Drosophila Proteins; D005796:Genes; D007814:Larva / Q000166:cytology; D009693:Nucleic Acid Hybridization; D011505:Protein-Tyrosine Kinases; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D011519:Proto-Oncogenes; D011679:Pupa / Q000166:cytology; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
D029721:Drosophila Proteins; D011518:Proto-Oncogene Proteins; D011505:Protein-Tyrosine Kinases; C075699:Src64B protein, Drosophila; D004262:DNA Restriction Enzymes