Cytoskeletons of ADP- and thrombin-stimulated blood platelets. Presence of a caldesmon-like protein, alpha-actinin and gelsolin at different steps of the stimulation.
Comparative analyses of the cytoskeletons of resting and stimulated platelets point out the involvement of a 79 kDa polypeptide in the activation step and its increased incorporation during aggregation. It appears as a doublet and cross-reacts with an antibody to chicken gizzard caldesmon, whereas no 150 kDa immunoreactive form was detected. alpha-Actinin and gelsolin were detected only in the aggregation step.
D000185:Actinin / Q000097:blood*; D000244:Adenosine Diphosphate / Q000494:pharmacology; D000818:Animals; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism*; D002135:Calcium-Binding Proteins / Q000097:blood*; D002148:Calmodulin-Binding Proteins / Q000097:blood*; D003599:Cytoskeleton / Q000378:metabolism; D018260:Gelsolin; D007120:Immunochemistry; D007074:Immunoglobulin G; D008840:Microfilament Proteins / Q000097:blood*; D010974:Platelet Aggregation / Q000187:drug effects; D013552:Swine; D013917:Thrombin / Q000494:pharmacology
D002135:Calcium-Binding Proteins; D002148:Calmodulin-Binding Proteins; D018260:Gelsolin; D007074:Immunoglobulin G; D008840:Microfilament Proteins; D000185:Actinin; D000244:Adenosine Diphosphate; D013917:Thrombin