Cloning, characterization and nucleotide sequences of two cDNAs encoding human pancreatic trypsinogens.
Two cDNA clones encoding two major human trypsinogen isozymes were isolated from a human pancreatic cDNA library. The deduced amino acid (aa) sequences of the two trypsinogen precursors are found to have 89% sequence homology, and have the same number of aa (247), including 15 aa for a signal peptide and 8 aa for an activation peptide. Southern blot analysis of human genomic DNA using the cloned cDNA as a probe, revealed that the human trypsinogen genes constitute a multigene family of more than ten genes.
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification*; D004262:DNA Restriction Enzymes; D006801:Humans; D007527:Isoenzymes / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D010179:Pancreas / Q000201:enzymology*; D014362:Trypsinogen / Q000235:genetics*
D007527:Isoenzymes; D014362:Trypsinogen; D004247:DNA; D004262:DNA Restriction Enzymes