Detection of human cytomegalovirus-induced DNA-binding proteins on Western blots.
DNA-binding proteins have been isolated from human embryonic fibroblast cells infected with human cytomegalovirus strain AD169 by native and denatured DNA-cellulose affinity chromatography and Polymin P precipitation. The DNA-binding proteins separated by polyacrylamide gel electrophoresis and electrophoretically transferred to a nitrocellulose sheet were identified by a modified ELISA reaction. Six DNA-binding proteins were found in infected cells, their molecular weights range from 52K to 18K.
D002478:Cells, Cultured; D003587:Cytomegalovirus / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000032:analysis / Q000096:biosynthesis / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005347:Fibroblasts / Q000382:microbiology; D006801:Humans; D007163:Immunosorbent Techniques
D004268:DNA-Binding Proteins