Inactivation of poliovirus with beta-propiolactone.
The recovery of poliovirus D-antigen after virus inactivation was studied for two inactivating agents (beta-propiolactone and formalin) using the three poliovirus types (Sabin types 1, 2 and 3). With beta-propiolactone (BPL), D-antigen recoveries were high (88, 88 and 60%, respectively) but were significantly less when formalin was used (22, 15 and 25%). beta-Propiolactone inactivated virus was purified, combined with Freund's adjuvant and used to hyperimmunize rabbits. High titres (50 000-200 000) of specific neutralizing antibody were obtained.
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D005557:Formaldehyde / Q000494:pharmacology; D007783:Lactones / Q000494:pharmacology*; D017955:Poliovirus / Q000187:drug effects / Q000276:immunology* / Q000472:pathogenicity; D011054:Poliovirus Vaccine, Inactivated / Q000276:immunology; D011420:Propiolactone / Q000494:pharmacology*; D011817:Rabbits; D014613:Vaccines, Attenuated; D014776:Virus Cultivation
D000956:Antigens, Viral; D007783:Lactones; D011054:Poliovirus Vaccine, Inactivated; D014613:Vaccines, Attenuated; D005557:Formaldehyde; D011420:Propiolactone