title: The yeast SRP gene: positive modulation by glucose of its transcriptional expression.
abstract: The yeast gene coding for a protein rich in serine residues (SRP) has been cloned. Its transcriptional expression is modulated positively by glucose as shown by Northern blots of mRNA prepared from cells grown in medium containing either glucose or raffinose or glycerol/ethanol as carbon sources. The positive elicitation of SRP mRNA by glucose was found to be associated with the promoter region of the SRP gene, using a plasmid with the lacZ gene fused with the SRP promoter region. The promoter region has been sequenced and disclosed all the characteristics of a strong yeast promoter.
mesh_terms: D001483:Base Sequence; D004262:DNA Restriction Enzymes / Q000378:metabolism; D000431:Ethanol / Q000494:pharmacology; D005656:Fungal Proteins / Q000235:genetics*; D005947:Glucose / Q000494:pharmacology*; D005990:Glycerol / Q000494:pharmacology; D006360:Heat-Shock Proteins; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000378:metabolism; D011887:Raffinose / Q000494:pharmacology; D014158:Transcription, Genetic / Q000187:drug effects*; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D005656:Fungal Proteins; D006360:Heat-Shock Proteins; D012333:RNA, Messenger; D000431:Ethanol; D004262:DNA Restriction Enzymes; D001616:beta-Galactosidase; D005947:Glucose; D011887:Raffinose; D005990:Glycerol