Neuroblastoma X glioma NG108-15 hybrid cells cultured in a serum-free chemically defined medium: effects on acute and chronic opiate regulation of adenylate cyclase activity.
Neuroblastoma X glioma NG108-15 hybrid cells cultured in a chemically defined medium within 3 cell passages, exhibited viability, growth rate and morphology similar to those of cells grown in medium supplemented with 5% fetal calf serum. Hybrid cells cultured in the chemically defined medium within these periods of time also did not exhibit a difference in basal adenylate cyclase activity nor in the enzymatic activities stimulated by adenosine, forskolin, NaF, GppNHp or Mn2+. Furthermore, opiate receptor density in chemically defined medium cultured cells remained identical to that in cells cultured in 5% fetal calf serum. The acute and chronic effects of opiates on adenylate cyclase were similar for cells grown under either set of conditions.
D000262:Adenylyl Cyclases / Q000378:metabolism*; D003470:Culture Media; D005048:Etorphine / Q000494:pharmacology*; D005910:Glioma; D006822:Hybrid Cells; D007981:Levorphanol / Q000494:pharmacology*; D009019:Morphinans / Q000494:pharmacology*; D009447:Neuroblastoma; D011957:Receptors, Opioid / Q000032:analysis
D003470:Culture Media; D009019:Morphinans; D011957:Receptors, Opioid; D007981:Levorphanol; D005048:Etorphine; D000262:Adenylyl Cyclases