2-deoxy-D-glucose inhibition of herpes simplex virus type-1 receptor expression.
Growth of HEp-2 cells in 2-deoxy-D-glucose (2-DOG) supplemented media decreased the cells' binding capacity for herpes simplex virus type-1 KOS(HSV-1) but not vesicular stomatitis virus. HEp-2 cells tolerated up to 30 mM 2-DOG, but 2-DOG was toxic for Vero cells over 2 mM. The reduction in binding was maintained for at least 24 h even after careful removal of the inhibitor and growth in normal media. Complete regeneration of the receptor sites on HEp-2 cells was observed 8 h after mild trypsinization of cells grown in either normal or the 2-DOG supplemented media. Specific glycoprotein characteristics of the HSV-1 binding site were indicated by its inactivation upon trypsinization (0.1 mg per 5 X 10(5) cells for 30 s) and blocking by wheat germ agglutinin but not limulin. These results suggest that 2-DOG inhibits the proper expression of cell surface glycoprotein HSV-1 receptor sites on HEp-2 cells.
D000818:Animals; D002294:Carcinoma, Squamous Cell; D002460:Cell Line; D002522:Chlorocebus aethiops; D003837:Deoxy Sugars / Q000494:pharmacology*; D003847:Deoxyglucose / Q000494:pharmacology*; D006023:Glycoproteins / Q000032:analysis; D006801:Humans; D007668:Kidney; D037102:Lectins / Q000494:pharmacology; D008565:Membrane Proteins / Q000032:analysis; D011991:Receptors, Virus / Q000096:biosynthesis* / Q000187:drug effects; D018139:Simplexvirus / Q000502:physiology*; D014357:Trypsin; D014721:Vesicular stomatitis Indiana virus / Q000502:physiology; D014909:Wheat Germ Agglutinins
D003837:Deoxy Sugars; D006023:Glycoproteins; D037102:Lectins; D008565:Membrane Proteins; D011991:Receptors, Virus; D014909:Wheat Germ Agglutinins; C015323:limulin; C049545:simplexvirus receptor; D003847:Deoxyglucose; D014357:Trypsin