An improved radioimmunoassay method for the detection of IgG antibodies against cytomegalovirus.
The non-specific binding seen with human sera in a radioimmunoassay for the detection of IgG antibodies specific for CMV can be reduced greatly by using a murine monoclonal antibody as a radiolabelled detecting antibody. Such non-specific binding formerly obtained with a polyclonal detecting antibody was due to the binding of the polyclonal reagent to factors on the solid phase other than IgG molecules.
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral / Q000032:analysis*; D003587:Cytomegalovirus / Q000276:immunology*; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D007074:Immunoglobulin G / Q000032:analysis*; D011863:Radioimmunoassay
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D007106:Immune Sera; D007074:Immunoglobulin G