title: A note on the use of protease inhibitors during chromatin fractionation on hydroxyapatite columns.
abstract: It was found that NaHSO3 present in the eluents enabled full and reproducible recovery of chromosomal proteins from a hydroxyapatite column. Another protease inhibitor, PMSF, did not have that effect.
mesh_terms: D000818:Animals; D002458:Cell Fractionation / Q000379:methods; D002843:Chromatin / Q000302:isolation & purification* / Q000648:ultrastructure; D002845:Chromatography / Q000379:methods; D017886:Durapatite; D006882:Hydroxyapatites; D008099:Liver / Q000648:ultrastructure; D008297:Male; D010664:Phenylmethylsulfonyl Fluoride; D051381:Rats; D011919:Rats, Inbred Strains; D013450:Sulfones
chemical_list: D002843:Chromatin; D006882:Hydroxyapatites; D013450:Sulfones; D010664:Phenylmethylsulfonyl Fluoride; D017886:Durapatite