Positive selection of activated T cells of the T8 (CD8) sub-type by immunomagnetic separation.
By conjugating a monoclonal IgM antibody of CD8-specificity to magnetite-containing polymer particles, we have developed a rapid and simple one-step procedure for positive selection of T8 cells. The method ensured good yield and viability of pure T8 cells. The positively selected T8-fraction from MLC activated T cells retained their cytolytic capacity and gave a moderate proliferative response. Most of the proliferative response occurred in the negatively selected T4 population which showed only a marginal cytotoxic activity against PHA blasts as target cells. Transferrin receptor expression, as measured in a direct radiobinding assay, could also be demonstrated both among pure T4 and T8 cells upon MLC-activation.
D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface / Q000276:immunology; D002469:Cell Separation / Q000379:methods; D002470:Cell Survival; D003602:Cytotoxicity, Immunologic; D006801:Humans; D008213:Lymphocyte Activation; D008280:Magnetics; D011956:Receptors, Cell Surface / Q000378:metabolism; D011990:Receptors, Transferrin; D013601:T-Lymphocytes / Q000145:classification* / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000145:classification / Q000276:immunology
D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface; D011956:Receptors, Cell Surface; D011990:Receptors, Transferrin