mRNA from human colon tumor and mucosa related to the pol gene of an endogenous A-type retrovirus.
The expression of endogenous retroviral genes in mammals may be etiologically related to genetic diseases including cancer. Recently, A-type human endogenous retroviral genomes have been cloned using the reverse transcriptase (pol) genes of rodent intracisternal A-particles (IAP). In this report, RNA from human colon adenocarcinoma and surrounding mucosa was hybridized to mouse IAP pol and gag genes to examine the expression of human endogenous A-type retroviruses. Abundant, heterogeneous size, polyadenylated transcripts homologous to the mouse IAP pol gene were detected in both tissues. Transcripts homologous to the mouse IAP gag region were not found.
D000230:Adenocarcinoma / Q000032:analysis / Q000235:genetics; D000818:Animals; D003001:Cloning, Molecular; D003110:Colonic Neoplasms / Q000032:analysis / Q000235:genetics*; D004262:DNA Restriction Enzymes / Q000378:metabolism; D005753:Gastric Mucosa / Q000032:analysis*; D005786:Gene Expression Regulation; D007426:Genes, Intracisternal A-Particle; D005814:Genes, Viral; D006801:Humans; D051379:Mice; D012333:RNA, Messenger / Q000032:analysis*; D012190:Retroviridae / Q000235:genetics*
D012333:RNA, Messenger; D004262:DNA Restriction Enzymes