Transformation of dog embryo kidney cells by human herpesviruses.
The infection of dog embryo kidney (DEK) cells with herpes simplex virus type 2 (HSV-2) or human cytomegalovirus (HCMV) led to the development of transformed cell lines. Rapidly dividing DEK cells with unlimited division potential exhibited growth in 2% serum, contained nuclear virus antigens, and formed small (+/- 0.2 mm) colonies in 0.3% agarose. Immortal cell lines showing the same transformation properties were also obtained after transfection with purified HSV-2 or HCMV DNA. These results confirm the transforming capacity of both herpesviruses as well as the usefulness of this different type of mammalian cells in transformation studies.
D000818:Animals; D002417:Cattle; D002453:Cell Cycle; D002460:Cell Line; D002472:Cell Transformation, Viral; D003587:Cytomegalovirus; D003586:Cytomegalovirus Infections / Q000473:pathology; D006561:Herpes Simplex / Q000473:pathology; D006566:Herpesviridae Infections / Q000473:pathology*; D007668:Kidney / Q000166:cytology* / Q000382:microbiology; D018139:Simplexvirus