title: Interleukin 2 increases T lymphocyte membrane mobility before the rise in cytosolic calcium concentration.
abstract: Using stopped-flow fluorometry with three different fluorescence probes [2-[(1-pyrenyl-butyryl)oxy]stearic acid, chlortetracycline and Quin 2], we have studied initial stage of T lymphocyte activation after interleukin 2 (IL-2) binding to a specific cell-surface receptor. After IL-2 binding to cytotoxic T lymphocyte (IL-2-dependent mouse LC7 and CTLL-2 cells), membrane mobilities of the cells increased first (4.5 +/- 0.3 s-1 for LC7 and 3.8 +/- 0.2 s-1 for CTLL-2), then calcium was released from intracellular stores into the cytoplasm (1.6 +/- 0.1 s-1 for LC7 and 2.1 +/- 0.1 s-1 for CTLL-2), and lastly, calcium was transported from the external medium into the cytoplasm (1.3 +/- 0.1 s-1 for LC7 and 1.5 +/- 0.1 s-1 for CTLL-2). The slowest process, the calcium influx from the external medium, was suppressed in the presence of a calcium channel blocking agent (verapamil). These observations give us a new information to discuss a model in T lymphocyte activation after IL-2 binding to cell-surface receptors.
mesh_terms: D000634:Aminoquinolines; D000818:Animals; D002118:Calcium / Q000097:blood*; D003600:Cytosol / Q000378:metabolism; D004578:Electron Spin Resonance Spectroscopy; D005434:Flow Cytometry; D005456:Fluorescent Dyes; D007376:Interleukin-2 / Q000276:immunology*; D008213:Lymphocyte Activation; D008560:Membrane Fluidity; D051379:Mice; D013602:T-Lymphocytes, Cytotoxic / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D014700:Verapamil / Q000494:pharmacology
chemical_list: D000634:Aminoquinolines; D005456:Fluorescent Dyes; D007376:Interleukin-2; D014700:Verapamil; C035739:Quin2; D002118:Calcium