Polyomavirus mutation that confers a cell-specific cis advantage for viral DNA replication.
The structural and biological properties of a polyomavirus mutant selected in Friend erythroleukemic cells were investigated. The growth efficiency of this mutant (PyFL78) was compared with that of the parental PyA2 strain by a growth competition assay in Friend erythroleukemic and 3T3 (or 3T6) cell lines. The results reveal that PyFL78 displays a cis-acting growth advantage over the PyA2 parental strain in Friend erythroleukemic cells but not in 3T3 or 3T6 cells. This cell-specific cis advantage is shown to be due to modifications within the polyomavirus noncoding regulatory region.
D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D004261:DNA Replication; D004262:DNA Restriction Enzymes; D051379:Mice; D009154:Mutation; D010957:Plasmids; D011120:Polyomavirus / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics
D012333:RNA, Messenger; D004262:DNA Restriction Enzymes