Effects of temperature on Epstein-Barr virus replication in epithelial/nasopharyngeal carcinoma hybrid cells.
The authors studied the replication of Epstein-Barr virus (EBV) in the epithelial hybrid cells derived from nasopharyngeal carcinoma (NPCKT) caused by the effects of sub-optimal incubation at 34, 32 and 28 degrees C, respectively, using an immunofluorescence technique. The intensity of EBV induction by sub-optimal temperatures was in the order of 32 degrees C----28 degrees C----34 degrees C. In particular, 18-24% positive cells for early antigen and viral capsid antigen were observed at around 10 to 14 days after shift-down of the incubation temperature from 37 to 32 degrees C.
D000818:Animals; D002460:Cell Line; D004848:Epithelium / Q000382:microbiology; D005455:Fluorescent Antibody Technique; D004854:Herpesvirus 4, Human / Q000502:physiology; D006801:Humans; D006822:Hybrid Cells / Q000382:microbiology; D009303:Nasopharyngeal Neoplasms / Q000382:microbiology*; D013696:Temperature; D014412:Tumor Virus Infections / Q000382:microbiology*; D014779:Virus Replication