Retention of iron by rat intestine in vivo as affected by dietary fiber, ascorbate and citrate.
The effects of pH, ascorbate, citrate and dietary fiber on retention of ferrous and ferric iron by jejuno-ileal segments of rat intestine were examined in vivo. Iron was introduced in an isosmotic solution of sodium chloride and dextrose buffered by 2-[bis(2-hydroxyethyl)amino]ethanesulfonic acid (BES) and acetate. Stabilization of the iron solutions was aided by use of iron concentrations less than or equal to 1 microgram/ml injected into the intestine for 10-min periods. Iron retention was optimal over a broad pH range from 5 to 7.8. Inclusion of ascorbic acid in the solution injected (5, 25 or 75 micrograms/ml) did not increase retention of iron in either valence state. A low concentration of sodium citrate (2 mM) had no effect on iron retention, but increasing the concentration to 5 mM released iron from the mucosa. Maize and wheat fibers decreased the retention of ferrous iron by binding and by promoting autoxidation and formation of poorly soluble iron polymers. Bound ferrous iron was released completely at pH below 5. Retention of ferric iron was also lowered in the presence of fiber, presumably as a result of polymerization. Retention of iron by the rat in the absence of ligands was independent of valence state.
D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology*; D001682:Biological Availability; D002951:Citrates / Q000494:pharmacology*; D019343:Citric Acid; D004043:Dietary Fiber / Q000494:pharmacology*; D005260:Female; D005290:Ferric Compounds / Q000378:metabolism; D005296:Ferrous Compounds / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007082:Ileum / Q000187:drug effects / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000187:drug effects; D007501:Iron / Q000378:metabolism*; D007583:Jejunum / Q000187:drug effects / Q000378:metabolism; D008297:Male; D000644:Quaternary Ammonium Compounds / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D014908:Triticum; D003313:Zea mays
D002951:Citrates; D004043:Dietary Fiber; D005290:Ferric Compounds; D005296:Ferrous Compounds; D000644:Quaternary Ammonium Compounds; D019343:Citric Acid; C038178:ammonium ferrous sulfate; D007501:Iron; C049467:ammonium ferric sulfate; D001205:Ascorbic Acid