Cloning and characterization of the streptothricin-resistance gene which encodes streptothricin acetyltransferase from Streptomyces lavendulae.
The streptothricin-resistance gene of Streptomyces lavendulae No. 1080 was cloned in S. lividans using pIJ41 as a vector. From subcloning experiments, the 1.6 kb BamH I fragment was determined to encode the structural gene. The cell extracts of S. lividans carrying the gene on the plasmid pKS7 had activity to inactivate streptothricin in the presence of S-acetyl coenzyme A, indicating that the gene product was streptothricin acetyltransferase.
D000123:Acetyltransferases / Q000235:genetics*; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D004352:Drug Resistance, Microbial; D005798:Genes, Bacterial; D013302:Streptomyces / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D013309:Streptothricins / Q000494:pharmacology
D004269:DNA, Bacterial; D013309:Streptothricins; D000123:Acetyltransferases; C044496:streptothricin acetyltransferase