title: Viral isolation versus immune staining of infected cell cultures for the laboratory diagnosis of herpes simplex virus infections.
abstract: The Difco Laboratories Cellmatics kit, an immune staining method for the detection of herpes simplex virus after amplification in culture, was found to be 94.6% (138 of 146) sensitive and 100% (302 of 302) specific. However, conventional culturing detected 10 (6.4%) additional viruses that were not herpes simplex. For a full-service diagnostic laboratory, viral isolation is important.
mesh_terms: D000818:Animals; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D006561:Herpes Simplex / Q000175:diagnosis*; D006801:Humans; D007124:Immunoenzyme Techniques; D011933:Reagent Kits, Diagnostic; D018139:Simplexvirus / Q000302:isolation & purification* / Q000502:physiology; D014709:Vero Cells
chemical_list: D011933:Reagent Kits, Diagnostic