title: Comparative susceptibilities of strain MRC-5 human embryonic lung fibroblast cells and the Cooney strain of human fetal tonsil cells for isolation of rhinoviruses from clinical specimens.
abstract: The comparative susceptibilities for rhinovirus isolation were determined for strain MRC-5 human embryonic lung fibroblast and human fetal tonsil cells. Samples from nasopharyngeal and throat swab clinical specimens were inoculated onto duplicate monolayers of each cell type. Of 105 rhinovirus-positive specimens, 78 (74%) were positive in MRC-5 cells, and 102 (97%) were positive in fetal tonsil cells (P less than 0.001). For 75 specimens positive in both, the mean time to cytopathic effect development with standard deviation was 1 +/- 2 days shorter in fetal tonsil cells (P less than 0.01). The use of a second virus culture increased rhinovirus isolations by 10% compared with a single culture.
mesh_terms: D002460:Cell Line; D002478:Cells, Cultured; D003588:Cytopathogenic Effect, Viral; D005333:Fetus; D005347:Fibroblasts; D006801:Humans; D008168:Lung; D009305:Nasopharynx / Q000382:microbiology; D014066:Palatine Tonsil; D010614:Pharynx / Q000382:microbiology; D012229:Rhinovirus / Q000254:growth & development / Q000302:isolation & purification*; D014776:Virus Cultivation