title: A peroxidase-coupled kinetic enzymatic procedure evaluated for measuring serum and urinary creatinine.
abstract: We evaluated an enzymatic procedure for the automated determination of serum and urinary creatinine. The procedure was sensitive and precise for serum creatinine concentrations as low as 3 mg/L, and the standard curve was linear to 200 mg/L. Measurements of creatinine in clinical serum and urine specimens agreed with those obtained by two other enzymatic methods and four picric acid methods. There was no interference from picrate-reactive substances tested. Among other substances, bilirubin (50-100 mg/L) interfered negatively with the assay, decreasing apparent creatinine concentrations by as much as 6 mg/L. The advantages of specificity, speed, and convenience make this enzymatic procedure an attractive primary or secondary method for creatinine determinations in clinical laboratories.
mesh_terms: D000328:Adult; D001663:Bilirubin / Q000032:analysis; D002648:Child; D003404:Creatinine / Q000032:analysis* / Q000097:blood / Q000652:urine; D005260:Female; D006801:Humans; D007202:Indicators and Reagents; D007527:Isoenzymes; D007700:Kinetics; D009195:Peroxidase; D010544:Peroxidases; D006435:Renal Dialysis
chemical_list: D007202:Indicators and Reagents; D007527:Isoenzymes; D003404:Creatinine; D010544:Peroxidases; D009195:Peroxidase; D001663:Bilirubin