Sendai virus replication in Friend erythroleukemia cells. I. Acutely and persistently infected cells become resistant to virus-induced lysis.
Friend leukemia cells (FLC) are susceptible to infection by Sendai virus, a member of the paramyxovirus group. FLC constitute a most suitable model to study virus-host cell interactions, because they grow in suspension (thus avoiding the use of trypsin), and provide an easy way of deriving single-cell clones. When FLC are infected with Sendai virus at high m.o.i., a direct, extensive lysis of the cells ensues, whereas lower doses of virus result in a cytocidal infection whose lethality depends mainly on the virus used, standard or defective interfering egg-grown Sendai virus (EGSV), and on the multiplicity of infection (m.o.i). At later times after infection, FLC become resistant to the Sendai induced lysis (SIL). The SIL resistance can be maintained in single-cell clones that had survived the first infection. The maintenance of the resistant phenotype of the clones requires the serial subcultivation of the cells in the presence of activated EGSV. The mechanisms that presumably regulate the appearance of SIL resistance in Sendai infected FLC are discussed.
D002459:Cell Fusion; D002460:Cell Line; D002470:Cell Survival; D002999:Clone Cells; D003588:Cytopathogenic Effect, Viral; D003673:Defective Viruses / Q000502:physiology; D005622:Friend murine leukemia virus; D006386:Hemagglutination Tests; D004915:Leukemia, Erythroblastic, Acute; D010222:Parainfluenza Virus 1, Human / Q000502:physiology*; D010641:Phenotype; D013696:Temperature; D010948:Viral Plaque Assay; D014764:Viral Proteins / Q000096:biosynthesis; D014779:Virus Replication
D014764:Viral Proteins