GABA mechanisms of ventromedial thalamic nucleus in morphine-induced muscle rigidity.
The aim of the study was to investigate the role of the ventromedial thalamic nucleus in the rigidity induced by morphine. Muscle rigidity was assessed using an electromyographic method (EMG) in non-anaesthesized rats with electrodes implanted unilaterally in the gastrocnemius soleus muscle. Subcutaneous (s.c.) injections of morphine in doses of 5, 10 or 20 mg/kg evoked tonic EMG activity in the gastrocnemius soleus muscle; this was estimated as muscle rigidity. Picrotoxin was injected bilaterally into the ventromedial nucleus in doses of 50-400 ng/0.5 microliter 30 min after morphine administration. Picrotoxin in doses of 200 and 400 ng attenuated the tonic EMG activity induced by morphine, 10 mg/kg s.c. Picrotoxin in a dose of 400 ng reduced the tonic activity induced by morphine, 20 mg/kg s.c. The results suggest that the thalamic ventromedial nucleus mediates the morphine-induced rigidity.
D000818:Animals; D008297:Male; D009020:Morphine / Q000037:antagonists & inhibitors / Q000633:toxicity*; D009127:Muscle Rigidity / Q000139:chemically induced* / Q000503:physiopathology; D010852:Picrotoxin / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D011963:Receptors, GABA-A / Q000187:drug effects* / Q000502:physiology; D014697:Ventromedial Hypothalamic Nucleus / Q000187:drug effects* / Q000503:physiopathology
D009020:Morphine; D010852:Picrotoxin; D011963:Receptors, GABA-A