[3H]5-hydroxytryptamine binding to brain astroglial cells: differences between intact and homogenized preparations and mature and immature cultures.
[3H]5-hydroxytryptamine ([3H]serotonin) binds with high affinity (KD 2-12 nM) to a finite number of sites on brain astroglial cells. The number of binding sites in the C6 glioma line is decreased significantly (Bmax = 315 versus 30 fmol/mg) by homogenization. In intact primary cultures, derived from newborn rat brain, the number of binding sites is far greater in cultures of immature astrocytes than in cultures treated with dibutyryl cyclic AMP (Bmax = 1,520 versus 580 fmol/mg). A role for these receptors in development is suggested.
D000818:Animals; D001253:Astrocytes / Q000187:drug effects / Q000378:metabolism*; D001921:Brain / Q000166:cytology*; D003994:Bucladesine / Q000494:pharmacology; D002460:Cell Line; D005910:Glioma / Q000378:metabolism; D051381:Rats; D012701:Serotonin / Q000378:metabolism*; D013997:Time Factors
D012701:Serotonin; D003994:Bucladesine