A study of the effects of catabolin on cyclic adenosine monophosphate biosynthesis and prostaglandin E2 secretion in pig articular chondrocytes.
The effects of porcine leucocyte catabolin on prostaglandin E2 (PGE2) secretion and cyclic adenosine monophosphate (cAMP) production were examined in pig articular chondrocytes. Cells were cultured in monolayers on plastic substrates for either 1 or 6 days. Catabolin was found to stimulate PGE2 secretion markedly in all chondrocyte cultures, but had no effect on cAMP biosynthesis, irrespective of the duration of culture, time of exposure or concentration. It is concluded that in pig articular chondrocytes catabolin does not modulate cell function by a cAMP-dependent mechanism.
D000818:Animals; D002358:Cartilage, Articular / Q000166:cytology / Q000378:metabolism*; D002478:Cells, Cultured; D003094:Collagen / Q000235:genetics; D003470:Culture Media; D000242:Cyclic AMP / Q000096:biosynthesis*; D015232:Dinoprostone; D007375:Interleukin-1 / Q000302:isolation & purification / Q000502:physiology*; D053583:Interleukin-1beta; D010641:Phenotype; D011458:Prostaglandins E / Q000378:metabolism*; D013552:Swine
D003470:Culture Media; D007375:Interleukin-1; D053583:Interleukin-1beta; D011458:Prostaglandins E; D003094:Collagen; D000242:Cyclic AMP; D015232:Dinoprostone