title: Plasmids supplying the Q-qut-controlled gam function permit growth of lambda red- gam- (Fec-) bacteriophages on recA- hosts.
abstract: A plasmid, pgam, has been constructed which expresses the phage lambda gene, gam, under the control of the lambda late promoter, p'R, contained in a form of a p'R-qut-t'R1 module. Lambda red- gam-, which normally do not grow on recA- hosts, are able to grow on recA- hosts containing pgam, because their Q function can turn on the gam gene expression. This facilitates cloning with lambda red- gam- vectors in recA- hosts.
mesh_terms: D010582:Bacteriophage lambda / Q000235:genetics*; D004262:DNA Restriction Enzymes; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D005814:Genes, Viral; D005838:Genotype; D009154:Mutation; D010957:Plasmids; D011401:Promoter Regions, Genetic
chemical_list: D004262:DNA Restriction Enzymes