Monoacylglycerol acyltransferase. Evidence that the activities from rat intestine and suckling liver are tissue-specific isoenzymes.
The monoglycerol acyltransferase (EC 2.3.1.22) (recommended name acylglycerol palmitoltransferase) activities from rat intestinal mucosa and suckling liver microsomes were compared in order to determine why substrate specificities differed in the two tissues. Suckling liver monoacylglycerol acyltransferase activity was highly specific for sn-2-mono-C18:1 glycerol and acylated rac-1-mono-C18:1 glycerol and 1- and 2-mono-C18:1 glycerol ethers poorly. In contrast, the substrate specificity of intestinal monoacylglycerol acyltransferase activity was broad. 1-Acyl- and 1- and 2-alkylglycerols were acylated at rates that were 45-78% of the rate observed with the preferred substrate sn-2-mono-C18:1 glycerol. Partial heat inactivation did not alter these relative specific activities, making it unlikely that intestinal microsomes contained a second acyltransferase capable of acylating the alternate substrates. The hypothesis that intestine and liver contain non-identical monoacylglycerol acyltransferase activities was further tested. Intestinal mucosa monoacylglycerol acyltransferase was much more thermolabile than the liver activity. Incubation with 50 microM diethylpyrocarbonate inactivated liver monoacylglycerol acyltransferase activity 84% but had little effect on the intestinal activity. Hydroxylamine completely reversed diethylpyrocarbonate inactivation, suggesting that critical histidine residues were more accessible in liver monoacylglycerol acyltransferase. 2,4,6-Trinitrobenzene sulfonic acid inactivated hepatic monoacylglycerol acyltransferase more than the intestinal activity, suggesting that critical lysine residues were more accessible. The intestinal and liver activities were also differently affected by acetone, detergents, MgCl2, phospholipids, and bovine serum albumin. Taken as a whole, the data strongly suggest that rat intestinal mucosa and suckling liver contain tissue-specific monoacylglycerol acyltransferase isoenzymes.
D000096:Acetone / Q000494:pharmacology; D000217:Acyltransferases / Q000378:metabolism* / Q000494:pharmacology; D000828:Animal Population Groups / Q000378:metabolism*; D000818:Animals; D000833:Animals, Suckling / Q000378:metabolism*; D002712:Chlorides; D003840:Deoxycholic Acid / Q000494:pharmacology; D004047:Diethyl Pyrocarbonate / Q000494:pharmacology; D006358:Hot Temperature; D007422:Intestines / Q000201:enzymology*; D007527:Isoenzymes / Q000378:metabolism*; D008099:Liver / Q000201:enzymology*; D008345:Manganese / Q000494:pharmacology; D017895:Manganese Compounds; D017830:Octoxynol; D010713:Phosphatidylcholines / Q000378:metabolism; D010718:Phosphatidylserines / Q000378:metabolism; D011092:Polyethylene Glycols / Q000494:pharmacology; D051381:Rats; D012710:Serum Albumin, Bovine / Q000378:metabolism; D013997:Time Factors; D014018:Tissue Distribution
D002712:Chlorides; D007527:Isoenzymes; D017895:Manganese Compounds; D010713:Phosphatidylcholines; D010718:Phosphatidylserines; D003840:Deoxycholic Acid; D000096:Acetone; D012710:Serum Albumin, Bovine; D011092:Polyethylene Glycols; D008345:Manganese; D017830:Octoxynol; D000217:Acyltransferases; C021802:2-acylglycerol O-acyltransferase; D004047:Diethyl Pyrocarbonate; C025340:manganese chloride