Postembedding immunogold labeling for electron microscopy using "LR White" resin.
A method is described for performing postembedding immunogold immunocytochemistry on sections of LR White-embedded tissues. Fixation of tissue in a combination of paraformaldehyde and glutaraldehyde, or with low concentrations of glutaraldehyde followed by partial dehydration, resulted in preservation of antigenicity for a variety of proteins in different tissue samples. Good structural preservation facilitated high-resolution immunolabeling when coupled with the use of purified monoclonal antibodies. The technique is straightforward and versatile, offering the potential for many immunocytochemical applications with minimal modifications.
D000818:Animals; D001457:Anion Exchange Protein 1, Erythrocyte / Q000378:metabolism; D000911:Antibodies, Monoclonal; D017301:Ca(2+) Mg(2+)-ATPase / Q000378:metabolism; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D004912:Erythrocytes / Q000648:ultrastructure; D005260:Female; D006046:Gold; D006801:Humans; D007120:Immunochemistry / Q000379:methods*; D015470:Leukemia, Myeloid, Acute / Q000201:enzymology; D008854:Microscopy, Electron / Q000379:methods*; D009132:Muscles / Q000201:enzymology / Q000648:ultrastructure; D009195:Peroxidase / Q000378:metabolism; D011817:Rabbits
D001457:Anion Exchange Protein 1, Erythrocyte; D000911:Antibodies, Monoclonal; D006046:Gold; D009195:Peroxidase; D017301:Ca(2+) Mg(2+)-ATPase; D000252:Calcium-Transporting ATPases