Photosensitive DNA cleavage and phage inactivation by copper(II)-camptothecin.
Upon irradiation with 365-nm light, copper(II)-camptothecin significantly produced single- and double-strand breaks of DNA and also induced a marked inactivation of bacteriophage. The nucleotide sequence analysis exhibited considerably random DNA cleavage. The DNA strand scission by the camptothecin-Cu(II)-UV light system, as well as the phage inactivation, was strongly suppressed by bathocuproine and catalase, indicating participation of cuprous species and hydrogen peroxide in the reaction. The present results suggest that (1) Cu(II) ion may play an important role as a cofactor in antitumor action of camptothecin and (2) the combination of copper-camptothecin plus long-wave ultraviolet light is useful against certain cancer treatment as a new photochemotherapy.
D010584:Bacteriophage phi X 174 / Q000187:drug effects* / Q000528:radiation effects; D002166:Camptothecin / Q000494:pharmacology*; D003300:Copper / Q000494:pharmacology*; D004279:DNA, Viral / Q000528:radiation effects*; D004578:Electron Spin Resonance Spectroscopy; D004926:Escherichia coli / Q000528:radiation effects; D007700:Kinetics; D009942:Organometallic Compounds; D010957:Plasmids / Q000528:radiation effects*; D014466:Ultraviolet Rays
D004279:DNA, Viral; D009942:Organometallic Compounds; C048684:camptothecin copper(II) complex; D003300:Copper; D002166:Camptothecin