Anti-complement immunofluorescence establishes nuclear localization of human cytomegalovirus matrix protein.
A monospecific, polyclonal antiserum to the 69-kDa matrix protein of human cytomegalovirus (HCMV) was prepared in a guinea pig and used to determine the intracellular distribution of this viral antigen. The resulting antiserum was specific for infected cells as tested by immunofluorescence, and specific for the HCMV matrix protein as determined by "nitrocellulose immunoassay" of electrophoretically separated, infected-cell proteins. Antibodies were reacted with fixed, infected human fibroblasts, and visualized by the anti-complement immunofluorescence procedure to avoid complications arising from the strong IgG Fc binding activity of the infected-cell-specific cytoplasmic inclusion. Results establish that the matrix protein is located in the nucleus, and indicate that it is concentrated in the nucleoplasm rather than within the intranuclear inclusions.
D000818:Animals; D003165:Complement System Proteins / Q000276:immunology; D003587:Cytomegalovirus / Q000032:analysis*; D005347:Fibroblasts; D005455:Fluorescent Antibody Technique; D006168:Guinea Pigs; D006801:Humans; D007106:Immune Sera; D008297:Male; D008970:Molecular Weight; D012867:Skin; D014763:Viral Matrix Proteins; D014764:Viral Proteins / Q000032:analysis*
D007106:Immune Sera; D014763:Viral Matrix Proteins; D014764:Viral Proteins; C047015:cytomegalovirus matrix protein, pp 64-69; D003165:Complement System Proteins