A new banding pattern of human chromosomes by in situ nick translation using ECO RI and biotin-dUTP.
Here we present first results of an attempt to replace the nicking activity of DNAase I by ECO RI for in situ nick translation of human chromosomes. For the detection of nick translated sites we used biotinylated dUTP. The procedure resulted in a distinct banding pattern of the chromosomes which does not seem to correspond to that reached by any known banding technique.
D001710:Biotin; D002871:Chromosome Banding / Q000379:methods*; D004261:DNA Replication; D004262:DNA Restriction Enzymes; D015246:Deoxyribonuclease EcoRI; D003856:Deoxyuracil Nucleotides; D006801:Humans; D006868:Hydrolysis; D008677:Metaphase
D003856:Deoxyuracil Nucleotides; D001710:Biotin; D004262:DNA Restriction Enzymes; D015246:Deoxyribonuclease EcoRI