Interaction between enzyme-generated triplet carbonyls and molecules intercalated into DNA.
The phosphorescence from enzyme-generated and -protected triplet acetone is very efficiently quenched by dyes intercalated into DNA. The process is unlikely to be due to energy transfer and is tentatively ascribed to electron transfer occurring within the DNA helix complex with the acting enzyme. This quenching markedly protects DNA from breaks induced by triplet acetone. In the case of some barely emissive enzyme-generated triplet carbonyl species, it is possible to detect a weak emission resulting from the interaction with dye X DNA; this emission may be associated with back electron transfer.
D000096:Acetone; D000447:Aldehydes; D001665:Binding Sites; D002865:Chromomycins; D004396:Coloring Agents; D004249:DNA Damage; D004579:Electron Transport; D004735:Energy Transfer; D007364:Intercalating Agents; D007527:Isoenzymes; D008433:Mathematics; D010100:Oxygen; D009195:Peroxidase; D010544:Peroxidases; D013050:Spectrometry, Fluorescence
D000447:Aldehydes; D002865:Chromomycins; D004396:Coloring Agents; D007364:Intercalating Agents; D007527:Isoenzymes; D000096:Acetone; C017439:isobutyraldehyde; D010544:Peroxidases; D009195:Peroxidase; D010100:Oxygen