Identification of a rat liver cDNA and mRNA coding for the 28 kDa gap junction protein.
By screening of a rat liver cDNA library with complex and deoxyinosine containing oligonucleotide probes a cDNA clone was isolated and shown by sequencing to code for the amino-terminal half of the rat liver 28 kDa gap junction protein. The insert hybridized to a 1.9 kb species from rat and mouse liver poly(A)+ RNA in Northern blot analysis. In embryonic mouse hepatocytes the amount of the 1.9 kb mRNA increased 3-fold between 24 and 96 h in culture. This correlates with the previously described increase of the 28 kDa gap junction protein under these conditions.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017630:Connexins; D004247:DNA / Q000302:isolation & purification*; D008099:Liver / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000302:isolation & purification*; D051381:Rats
D017630:Connexins; D008565:Membrane Proteins; D012333:RNA, Messenger; D004247:DNA