title: Hydroxyl radicals are not involved in NADPH dependent microsomal lipid peroxidation.
abstract: NADPH dependent H2O2 formation in microsomes in the presence of chelated iron leads to formation of hydroxyl radicals. Enhancement of hydroxyl radical generation (via ferric-EDTA or sodium azide) did not result in a concomitant increase in lipid peroxidation; rather, a decrease was observed. Moreover, the hydroxyl radical scavenger DMSO did not inhibit lipid peroxidation. This comparison of hydroxyl radical formation with lipid peroxidation suggests that hydroxyl radicals do not play a part in NADPH-dependent lipid peroxidation.
mesh_terms: D000818:Animals; D001386:Azides / Q000494:pharmacology; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D005290:Ferric Compounds / Q000494:pharmacology; D006878:Hydroxides / Q000378:metabolism*; D017665:Hydroxyl Radical; D008054:Lipid Peroxides / Q000378:metabolism*; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism*; D009249:NADP / Q000494:pharmacology*; D010084:Oxidation-Reduction; D051381:Rats; D011919:Rats, Inbred Strains; D019810:Sodium Azide
chemical_list: D001386:Azides; D005290:Ferric Compounds; D006878:Hydroxides; D008054:Lipid Peroxides; D017665:Hydroxyl Radical; D009249:NADP; D019810:Sodium Azide; D004492:Edetic Acid; C019179:Fe(III)-EDTA; D004121:Dimethyl Sulfoxide