[Production of infectious virus and the accumulation in Vero cells of Pichinde virus antigens detectable by an indirect fluorescent antibody method].
The dynamics of accumulation of infectious Pichinde virus in the culture medium and virus-specific antigens in cells was studied in relation to multiplicity of infection in a multicycle experiment. Differences in the fluorescence pattern of Pichinde virus antigens in IFAT were found to depend on the use of acetone or formaldehyde for fixation of the infected cells.
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D001116:Arenaviridae / Q000254:growth & development*; D006477:Arenaviruses, New World / Q000254:growth & development* / Q000276:immunology; D005455:Fluorescent Antibody Technique; D007106:Immune Sera / Q000302:isolation & purification; D007114:Immunization; D007700:Kinetics; D051379:Mice; D013997:Time Factors; D014709:Vero Cells; D014776:Virus Cultivation
D000956:Antigens, Viral; D007106:Immune Sera
Produktsiia infektsionnogo virusa i nakoplenie v kletkakh Vero antigenov virusa Pichinda, vyiavliaemykh nepriamym metodom fliuorestsiruiushchikh antitel.