Lack of inhibitory effect of alpha-human atrial natriuretic polypeptide on aldosteronogenesis in aldosterone-producing adenoma.
The effects of synthetic alpha-human atrial natriuretic polypeptide (alpha hANP) on aldosteronogenesis in normal and aldosterone-producing adenoma cells (APA cells) in primary monolayer cultures were studied. alpha hANP significantly inhibited aldosterone secretion from normal adrenal cells in culture, but had no inhibitory effect on aldosterone secretion from APA cells in the presence or absence of 10(-8) M ACTH. alpha hANP enhanced the accumulation of intracellular cGMP in normal adrenal cells in culture, but not in APA cells. Visualization of [125I]iodo-alpha hANP-specific binding sites in APA and adjacent normal adrenal tissues by an in vitro receptor autoradiographic technique showed that these sites were localized only in normal adrenal tissue, but not in APA tissue. These results suggest that the lack of an inhibitory effect of alpha hANP on aldosteronogenesis in APA cells may be due to the absence of alpha hANP-specific receptor sites in APA cells.
D000236:Adenoma / Q000378:metabolism*; D000306:Adrenal Cortex Neoplasms / Q000378:metabolism*; D000328:Adult; D000450:Aldosterone / Q000096:biosynthesis* / Q000378:metabolism; D009320:Atrial Natriuretic Factor / Q000494:pharmacology*; D001345:Autoradiography; D002478:Cells, Cultured; D006152:Cyclic GMP / Q000096:biosynthesis; D005260:Female; D006801:Humans; D008875:Middle Aged; D011869:Radioligand Assay; D017461:Receptors, Atrial Natriuretic Factor; D011956:Receptors, Cell Surface / Q000378:metabolism
D011956:Receptors, Cell Surface; D000450:Aldosterone; D009320:Atrial Natriuretic Factor; D017461:Receptors, Atrial Natriuretic Factor; D006152:Cyclic GMP