Association of increased polyamine levels with isoproterenol-stimulated mucin secretion in the rat submandibular gland.
Incubation of rat submandibular gland slices with 50 microM isoproterenol for 10-40 min stimulated mucin secretion and induced a 3- to 4-fold increase in tissue concentrations of the polyamines putrescine, spermidine and spermine. alpha-Difluoromethylornithine, a specific inhibitor of ornithine decarboxylase, suppressed the isoproterenol-induced increase in submandibular polyamines and inhibited mucin secretion. Exogenous putrescine restored tissue polyamine levels and partially reversed the inhibitory effect of alpha-difluoromethylornithine on mucin secretion. Rapid increases in polyamine levels appear to mediate isoproterenol-stimulated mucin secretion in the rat submandibular gland.
D000818:Animals; D000518:Eflornithine; D005944:Glucosamine / Q000378:metabolism; D007545:Isoproterenol / Q000494:pharmacology*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008297:Male; D009077:Mucins / Q000378:metabolism*; D009952:Ornithine / Q000031:analogs & derivatives / Q000494:pharmacology; D011073:Polyamines / Q000378:metabolism*; D011700:Putrescine / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D011943:Receptors, Adrenergic, beta / Q000378:metabolism; D013363:Submandibular Gland / Q000187:drug effects / Q000378:metabolism*; D013997:Time Factors
D009077:Mucins; D011073:Polyamines; D011943:Receptors, Adrenergic, beta; D009952:Ornithine; D007770:L-Lactate Dehydrogenase; D007545:Isoproterenol; D005944:Glucosamine; D011700:Putrescine; D000518:Eflornithine