title: Characterization of the DNA of the hamster papovavirus: IV. Transcription mapping of calf-thymus DNA polymerase II.
abstract: Nascent RNA, synthesized by calf thymus RNA polymerase II on restriction endonuclease BamHI linearized hamster papovavirus (HaPV) DNA, was rehybridized to the template strand under conditions allowing transcription R-loop formation. Hybrids, visualized by electron microscopy, were plotted and mapped according to the physical map of HaPV. Two predominant regions of transcription could be localized at 0.10--0.40 and 0.50--0.82 m.u., respectively. For the start sites of transcription at map positions 0.67 and 0.75, respectively, on the HaPV genome a transcription in opposite direction were estimated. This genome region harbours the putative origin of replication of HaPV DNA. These results suggest a distinct relatedness of HaPV to the polyomavirus group.
mesh_terms: D000818:Animals; D002417:Cattle; D002874:Chromosome Mapping; D006224:Cricetinae; D004257:DNA Polymerase II / Q000378:metabolism*; D004262:DNA Restriction Enzymes / Q000494:pharmacology; D004279:DNA, Viral / Q000235:genetics*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D015245:Deoxyribonuclease BamHI; D008647:Mesocricetus; D008854:Microscopy, Electron; D009693:Nucleic Acid Hybridization / Q000187:drug effects; D027383:Papillomaviridae / Q000145:classification / Q000235:genetics* / Q000648:ultrastructure; D027382:Polyomaviridae; D012367:RNA, Viral / Q000096:biosynthesis; D013950:Thymus Gland / Q000201:enzymology*; D014158:Transcription, Genetic
chemical_list: D004279:DNA, Viral; D012367:RNA, Viral; D012321:DNA-Directed RNA Polymerases; D004257:DNA Polymerase II; D004262:DNA Restriction Enzymes; D015245:Deoxyribonuclease BamHI