Localization of beta-adrenoceptors in the rabbit ear by light microscopic autoradiography.
The beta-adrenoceptor antagonist [125I]cyanopindolol (CYP) was used to localize beta-adrenoceptors in sections of rabbit ear. Biochemical studies demonstrated that the binding was stereoselective, and that the beta-adrenoceptors are predominantly of the beta 2-subtype. Autoradiographic studies using 3H-Ultrofilm or nuclear emulsion coated coverslips showed high concentrations of beta 2-adrenoceptors present in the central ear artery, hyaline cartilage, nerve trunks, epithelium and sebaceous glands.
D000818:Animals; D001345:Autoradiography; D015784:Betaxolol; D002467:Cell Nucleus / Q000378:metabolism; D004431:Ear, External / Q000098:blood supply*; D066298:In Vitro Techniques; D007457:Iodine Radioisotopes; D020120:Iodocyanopindolol; D008853:Microscopy; D010869:Pindolol / Q000031:analogs & derivatives / Q000378:metabolism; D011412:Propanolamines / Q000494:pharmacology; D051381:Rats; D011943:Receptors, Adrenergic, beta / Q000378:metabolism*
D007457:Iodine Radioisotopes; D011412:Propanolamines; D011943:Receptors, Adrenergic, beta; D020120:Iodocyanopindolol; D010869:Pindolol; D015784:Betaxolol