Further study of hydroxyapatite high-performance liquid chromatography using both proteins and nucleic acids, and a new technique to increase chromatographic efficiency.
Previously developed hydroxyapatite high-performance liquid chromatography columns were tested further by using not only proteins but also nucleic acids. In both cases it was confirmed that the column can, in fact, discriminate subtle structural differences among molecules. Especially for protein mixtures a new technique was introduced to increase the efficiency of chromatography.
D000818:Animals; D001842:Bone and Bones / Q000032:analysis; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003577:Cytochrome P-450 Enzyme System / Q000032:analysis; D017886:Durapatite; D006801:Humans; D006882:Hydroxyapatites; D007782:Lactoglobulins / Q000032:analysis; D008099:Liver / Q000201:enzymology; D009696:Nucleic Acids / Q000032:analysis*; D011506:Proteins / Q000032:analysis*; D012346:RNA, Transfer, Amino Acyl / Q000032:analysis; D051381:Rats; D012463:Saliva / Q000032:analysis
D006882:Hydroxyapatites; D007782:Lactoglobulins; D009696:Nucleic Acids; D011506:Proteins; D012346:RNA, Transfer, Amino Acyl; D003577:Cytochrome P-450 Enzyme System; D017886:Durapatite