Biosynthesis of beta-endorphin in pancreatic islets of neonatal Wistar rats.
Pancreatic islets of neonatal Wistar rats were isolated by collagenase digestion and incubated with [14C]-L-prolin. Using a specific beta-endorphin antiserum radioactivity was found in the antibody-antigen-complex. The radioactivity was displaced by unlabelled beta-endorphin. These findings give the first evidence for the biosynthesis of beta-endorphin or beta-endorphin-like peptides in mammalian pancreatic islets.
D000818:Animals; D000831:Animals, Newborn; D004723:Endorphins / Q000096:biosynthesis*; D007515:Islets of Langerhans / Q000378:metabolism*; D003012:Microbial Collagenase; D011392:Proline / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D001615:beta-Endorphin
D004723:Endorphins; D001615:beta-Endorphin; D011392:Proline; D003012:Microbial Collagenase