Isolation and characterization of plasmids from Micromonospora zionensis and Micromonospora rosaria.
Three plasmids from Micromonospora species were isolated and characterized. Micromonospora zionensis NRRL5466 (a producer of sisomicin and G-52) carried a high-copy-number plasmid pMZ1 (9.9 kb). Micromonospora rosaria NRRL3718 (a producer of rosamicin) contained a large plasmid, pMR1 (53.5 kb), and a relatively small plasmid, pMR2 (11.0 kb).
D000900:Anti-Bacterial Agents; D002874:Chromosome Mapping; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000235:genetics; D007933:Leucomycins / Q000235:genetics; D008847:Micromonospora / Q000235:genetics*; D010957:Plasmids; D012853:Sisomicin / Q000031:analogs & derivatives / Q000235:genetics
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D007933:Leucomycins; C012632:6'-N-methylsisomicin; D004262:DNA Restriction Enzymes; D012853:Sisomicin