Chemical crosslinking of different forms of the simian virus 40 large T antigen using bifunctional reagents.
Chemical crosslinking was used for a direct analysis of the different forms of large tumor (T) antigen, the simian virus 40 A gene product. The first subclass, sedimenting at 14-16S, is composed of monomeric to tetrameric units, whereas the second, sedimenting at 5-6S, only contains dimers and monomers of T. The occurrence of oligomeric structures of T in solution which are higher than dimers suggests the possibility of direct binding of such trimers or tetramers to the origin of replication of the viral DNA as an alternative to the formation of these structures by aggregation of bound dimers or monomers after their sliding along the DNA.
D000952:Antigens, Polyomavirus Transforming; D000957:Antigens, Viral, Tumor / Q000378:metabolism*; D003432:Cross-Linking Reagents / Q000494:pharmacology*; D004261:DNA Replication; D004279:DNA, Viral / Q000378:metabolism*; D004120:Dimethyl Suberimidate / Q000494:pharmacology; D046911:Macromolecular Substances; D009856:Oncogene Proteins, Viral / Q000378:metabolism*; D013539:Simian virus 40 / Q000235:genetics*
D000952:Antigens, Polyomavirus Transforming; D000957:Antigens, Viral, Tumor; D003432:Cross-Linking Reagents; D004279:DNA, Viral; D046911:Macromolecular Substances; D009856:Oncogene Proteins, Viral; D004120:Dimethyl Suberimidate