title: The assay of ATP-sulfurylase.
abstract: An assay method for ATP-sulfurylase is described, based on the incorporation of 35SO4(2-) into adenosine-5'-phosphosulfate (and 3-phosphoadenosine-5'-phosphosulfate) and the separation of these compounds by HPLC. Since the enzyme is easily inactivated at temperatures above 20 degrees C during the assay, the reaction time should not exceed 10 min.
mesh_terms: D000250:Adenosine Phosphosulfate; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D008099:Liver / Q000201:enzymology; D009713:Nucleotidyltransferases / Q000032:analysis*; D010724:Phosphoadenosine Phosphosulfate; D051381:Rats; D013430:Sulfate Adenylyltransferase / Q000032:analysis*; D013431:Sulfates
chemical_list: D013431:Sulfates; D010724:Phosphoadenosine Phosphosulfate; D000250:Adenosine Phosphosulfate; D009713:Nucleotidyltransferases; D013430:Sulfate Adenylyltransferase