Studies on the protein and antigen composition of individual female Onchocerca volvulus after collagenase digestion.
The composition of proteins and antigens of female Onchocerca volvulus from one focus of transmission was studied by SDS-PAGE and immunoblotting. Worms that had been exposed to collagenase digestion of onchocercomata for different periods of time and parasites of different age and status of reproduction were tested. Some O. volvulus antigens were found to be sensible to prolonged digestion (molecular weights: 18 KD, 21 KD, 24 KD) but the majority of the antigens was stable up to 64 hours of incubation at 34 degrees C. The composition of proteins and antigens varied with the age and the status of reproduction of the worms. Slight differences between individual filariae were found, even when worms of comparable age and status of reproduction were tested that had been exposed to nodule digestion for comparable time.
D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis* / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007163:Immunosorbent Techniques; D003012:Microbial Collagenase; D008970:Molecular Weight; D009854:Onchocerca / Q000032:analysis / Q000276:immunology*; D011506:Proteins / Q000032:analysis* / Q000302:isolation & purification; D013997:Time Factors
D000947:Antigens, Helminth; D011506:Proteins; D003012:Microbial Collagenase