Beta-adrenergic stimulation enhances translocation, processing and synthesis of lipoprotein lipase in rat heart cells.
Cells isolated from newborn rat hearts were cultured for 10-14 days, and lipoprotein lipase activity was present in an intracellular and heparin-releasable pool. Treatment of the cultures with 10(-7) M isoproterenol for 3 min resulted in a 3-fold increase in heparin-releasable lipoprotein lipase and a concomitant decrease in residual cellular enzyme activity. Similar results were obtained by treatment with dibutyryl cAMP. Treatment with isoproterenol or dibutyryl cAMP for 2 h affected glycosylation of immunoadsorbable lipoprotein lipase, so that the ratio of [3H]galactose to [14C]mannose in the heparin-releasable enzyme increased from 3.8 (control) to 13.0 (isoproterenol-treated). The change in the ratio of the sugars in the cellular fraction of the enzyme was from 3.1 to 9.9. 2 h treatment with isoproterenol did not enhance new enzyme synthesis, as determined by incorporation of [3H]leucine into immunoadsorbable lipoprotein lipase. 24 h after addition of either isoproterenol or dibutyryl cAMP to the culture medium, stimulation of enzyme synthesis was demonstrated. The present results permit three effects of isoproterenol on lipoprotein lipase to be distinguished: stimulation of translocation from a cellular to heparin-releasable pool; enhanced processing of mannose residues and terminal glycosylation; stimulation of synthesis of enzyme protein.
D000818:Animals; D000831:Animals, Newborn; D003994:Bucladesine / Q000494:pharmacology; D002478:Cells, Cultured; D005690:Galactose / Q000378:metabolism; D006493:Heparin / Q000494:pharmacology; D007545:Isoproterenol / Q000494:pharmacology; D007930:Leucine / Q000378:metabolism; D008071:Lipoprotein Lipase / Q000378:metabolism*; D008358:Mannose / Q000378:metabolism; D009206:Myocardium / Q000201:enzymology*; D051381:Rats; D011943:Receptors, Adrenergic, beta / Q000378:metabolism*; D013997:Time Factors
D011943:Receptors, Adrenergic, beta; D003994:Bucladesine; D006493:Heparin; D008071:Lipoprotein Lipase; D007930:Leucine; D007545:Isoproterenol; D008358:Mannose; D005690:Galactose