Improved antigen detection in ethanol-fixed cytologic specimens. A modified avidin-biotin-peroxidase complex (ABC) method.
A new amplification technique is described for the detection of small amounts of antigen in ethanol-fixed cytologic specimens and formalin-fixed tissues. By adding a third antibody layer to the already sensitive avidin-biotin immunoperoxidase system, we have demonstrated a considerable increase in both the quantitative and qualitative aspects of this immunostaining method. In evaluating this technique, we employed a previously defined small cell lung carcinoma cell surface antigen system and monoclonal antibodies.
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm / Q000032:analysis*; D001360:Avidin; D001710:Biotin; D018288:Carcinoma, Small Cell / Q000276:immunology*; D006651:Histocytochemistry; D006801:Humans; D007124:Immunoenzyme Techniques; D007527:Isoenzymes; D008175:Lung Neoplasms / Q000276:immunology*; D010047:Ovalbumin / Q000031:analogs & derivatives; D009195:Peroxidase; D010544:Peroxidases
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D007527:Isoenzymes; D001360:Avidin; D001710:Biotin; D010047:Ovalbumin; D010544:Peroxidases; D009195:Peroxidase