Studies on rat liver mitochondria. 6. The effect of contaminating particles in mitochondria stored at 0-4 degrees C.
Rat liver mitochondria were stored at 0-4 degrees C for several days using an appropriate medium and energy source. The elimination of the majority of microsomes and lysosomes, that normally contaminate isolated mitochondria, had a positive effect in preservation of respiratory control, P:O ratio, and monoamine oxidase activity during long term storage.
D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D002458:Cell Fractionation / Q000379:methods; D003080:Cold Temperature; D005230:Fatty Acids, Nonesterified / Q000378:metabolism; D005952:Glucose-6-Phosphatase / Q000378:metabolism; D008854:Microscopy, Electron; D008930:Mitochondria, Liver / Q000502:physiology* / Q000648:ultrastructure; D008995:Monoamine Oxidase / Q000378:metabolism; D010085:Oxidative Phosphorylation; D010743:Phospholipids / Q000378:metabolism; D011309:Preservation, Biological; D051381:Rats
D005230:Fatty Acids, Nonesterified; D010743:Phospholipids; D008995:Monoamine Oxidase; D000135:Acid Phosphatase; D005952:Glucose-6-Phosphatase