title: Effects of tumour promoters on metabolic cooperation between human hepatoma cells.
abstract: Metabolic cooperation between cells from three human hepatoma cell lines was studied by the clonogenic method and by autoradiography. It was found that human HGPRT+/HGPRT- SK-HEP-1 cells only, showed a metabolic cooperation capacity that was inhibited by tumour promoters 12-O-tetradecanoylphorbol-13-acetate (TPA) and phenobarbital, and was not inhibited by the non-promoter 4-O-methyl TPA, provided suitable experimental conditions (short exposure times) were used. This biological system might be the basis of a new in vitro short-term screening test for potential tumour-promoting chemicals.
mesh_terms: D002273:Carcinogens / Q000494:pharmacology*; D006528:Carcinoma, Hepatocellular / Q000378:metabolism* / Q000648:ultrastructure; D002450:Cell Communication; D002460:Cell Line; D006801:Humans; D007041:Hypoxanthine Phosphoribosyltransferase / Q000032:analysis; D008113:Liver Neoplasms / Q000378:metabolism* / Q000648:ultrastructure; D010634:Phenobarbital / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate / Q000031:analogs & derivatives / Q000494:pharmacology
chemical_list: D002273:Carcinogens; C020922:4-O-methyl-12-O-tetradecanoylphorbol 13-acetate; D007041:Hypoxanthine Phosphoribosyltransferase; D013755:Tetradecanoylphorbol Acetate; D010634:Phenobarbital