Characterization of membrane-associated growth inhibitor activity for Ehrlich ascites mammary carcinoma cells.
Purified plasma membranes obtained from bovine milk or from bovine mammary gland inhibited in vitro growth of Ehrlich ascites mammary carcinoma cells. Plasma membranes derived from tumor cells or from tumor tissue were inactive. Growth inhibition by milk fat globule membranes was shown to be due to release of membrane-associated inhibitory factors into the medium. Gentle removal of proteins from the surface of milk fat globule membranes succeeded in recovery of a fraction inhibiting cell growth specifically. The same fraction contained the antigen cross-reacting with an antiserum raised against the previously purified 13 kD growth inhibitor from bovine mammary gland.
D000818:Animals; D017301:Ca(2+) Mg(2+)-ATPase / Q000378:metabolism; D002286:Carcinoma, Ehrlich Tumor / Q000473:pathology* / Q000503:physiopathology; D002453:Cell Cycle; D002462:Cell Membrane / Q000502:physiology*; D004797:Enzyme-Linked Immunosorbent Assay; D005966:Glucuronidase / Q000378:metabolism; D006131:Growth Inhibitors / Q000502:physiology*; D006801:Humans; D008321:Mammary Glands, Animal / Q000648:ultrastructure; D008565:Membrane Proteins / Q000276:immunology / Q000502:physiology; D018396:Mucin-1; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013385:Succinate Dehydrogenase / Q000378:metabolism
D006131:Growth Inhibitors; D008565:Membrane Proteins; D018396:Mucin-1; D013385:Succinate Dehydrogenase; D005966:Glucuronidase; D017301:Ca(2+) Mg(2+)-ATPase; D000254:Sodium-Potassium-Exchanging ATPase