A comparison of the dodecyl sulfate-induced precipitation of the myelin basic protein with other water-soluble proteins.
The interactions of sodium dodecyl sulfate with a number of proteins were examined at a variety of pH values ranging from 4.8 to 11.6. The dodecyl sulfate-induced precipitation of some of these proteins was observed within a relatively limited range of total dodecyl sulfate concentration. Most of the basic proteins precipitated at low pH but as the isoelectric point of the protein was approached the amount of protein that precipitated decreased. Bovine myelin basic protein was unique in that it precipitated at all pH values examined both above and below its isoelectric point. Thus, the dodecyl sulfate-induced precipitation of myelin basic protein appears to be different from the dodecyl sulfate-induced precipitation of most proteins. A comparison of protein precipitation at equivalent dodecyl sulfate:protein molar or weight ratios revealed very little difference in the precipitation behavior of the proteins studied. When the bovine myelin basic protein was cleaved at its single tryptophan residue, the N-terminal fragment (1-115) formed insoluble dodecyl sulfate complexes at pH values ranging from 4.8 to 9.2. The C-terminal fragment (116-169) precipitated almost completely at pH 4.8 but to a lesser extent at pH 7.4 and 9.2. Equimolar mixtures of the N- and C-terminal fragments precipitated in the presence of dodecyl sulfate at pH 7.4 and 9.2 to an extent greater than the C-terminal fragment alone but comparable to the N-terminal fragment alone or the whole basic protein. These results suggest: that the mechanism by which dodecyl sulfate induces the precipitation of myelin basic protein may be unique compared to other proteins and that the intact myelin basic protein is not necessary for its precipitation by dodecyl sulfate.
D000818:Animals; D001921:Brain / Q000378:metabolism; D002417:Cattle; D055598:Chemical Phenomena; D011232:Chemical Precipitation; D002627:Chemistry, Physical; D003574:Cytochrome c Group / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D009113:Muramidase / Q000378:metabolism; D009185:Myelin Proteins; D012260:Ribonucleases / Q000378:metabolism; D012709:Serum Albumin / Q000378:metabolism; D012862:Skatole / Q000031:analogs & derivatives / Q000494:pharmacology; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology*; D012995:Solubility; D014357:Trypsin / Q000378:metabolism
D003574:Cytochrome c Group; D009185:Myelin Proteins; D012709:Serum Albumin; C005904:BNPS-skatole; D012967:Sodium Dodecyl Sulfate; D012862:Skatole; D012260:Ribonucleases; D009113:Muramidase; D014357:Trypsin