Isolation of genes active during hormone-induced morphogenesis in Drosophila imaginal discs.
Mass-isolated Drosophila imaginal discs cultured in vitro undergo morphogenesis (evagination) in response to the insect steroid hormone 20-hydroxyecdysone. In vitro translation of mRNA isolated from evaginating discs shows accumulation of at least five new mRNA transcripts that are present only in membrane-bound polysomal RNA and presumably encode imaginal disc membrane or secreted proteins. Using a modified differential hybridization screen employing a competition step, six different hormone-inducible membrane protein gene sequences were isolated. These genes constitute a new set of 20-hydroxyecdysone responsive loci that may encode gene products specifically required for imaginal disc morphogenesis.
D000818:Animals; D004247:DNA / Q000032:analysis; D004262:DNA Restriction Enzymes / Q000378:metabolism; D015246:Deoxyribonuclease EcoRI; D004330:Drosophila; D004440:Ecdysone / Q000494:pharmacology; D004441:Ecdysterone / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects*; D008565:Membrane Proteins / Q000235:genetics; D008854:Microscopy, Electron; D009024:Morphogenesis / Q000187:drug effects*; D009693:Nucleic Acid Hybridization; D011132:Polyribosomes / Q000032:analysis; D014176:Protein Biosynthesis / Q000187:drug effects*; D012333:RNA, Messenger / Q000378:metabolism
D008565:Membrane Proteins; D012333:RNA, Messenger; D004440:Ecdysone; D004441:Ecdysterone; D004247:DNA; D004262:DNA Restriction Enzymes; D015246:Deoxyribonuclease EcoRI