title: The putative transforming protein of S13 avian erythroblastosis virus is a transmembrane glycoprotein with an associated protein kinase activity.
abstract: S13 is an avian retrovirus that transforms both fibroblasts and erythroblasts. The gene product responsible for the oncogenic effects of S13 is the env-related glycoprotein gp155. In this report we show that gp155 is a transmembrane protein with a 55-kDa cytoplasmic domain. Pulse-chase analysis shows that gp155 was cleaved posttranslationally into two glycosylated proteins, gp85 and gp70. In addition, we show that a tyrosine protein kinase activity is associated only with the gp70 protein in microsomes and in immune complexes.
mesh_terms: D001355:Alpharetrovirus / Q000235:genetics*; D000818:Animals; D001354:Avian Leukosis Virus / Q000235:genetics*; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D002645:Chickens; D006023:Glycoproteins / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D008970:Molecular Weight; D009856:Oncogene Proteins, Viral / Q000276:immunology / Q000502:physiology*; D011487:Protein Conformation; D011494:Protein Kinases / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D014759:Viral Envelope Proteins / Q000276:immunology / Q000502:physiology*
chemical_list: D006023:Glycoproteins; D008565:Membrane Proteins; D009856:Oncogene Proteins, Viral; D014759:Viral Envelope Proteins; D011494:Protein Kinases