A sequence in HpaI-P fragment of herpes simplex virus-1 DNA determines intraperitoneal virulence in mice.
The virulence of herpes simplex virus-1 (HSV-1) strains by the intraperitoneal (ip) route of injection in mice depends on the presence of an intact sequence in the HpaI DNA fragment P within coordinates 0.762 to 0.787. Deletion of the HpaI-P region (e.g., strain HFEM) abrogates the ability of the virus to infect mice by the ip route without affecting pathogenicity by the intracerebral (ic) route. A recombinant virus (M1C1) derived from DNA of the HSV-1 HFEM strain and the MLUIDNA fragment (coordinates 0.761 to 0.796) spanning the HpaI-P sequence of the pathogenic strain F regained pathogenicity for mice by the ip route.
D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000382:microbiology; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant; D004279:DNA, Viral / Q000235:genetics*; D015252:Deoxyribonucleases, Type II Site-Specific; D005814:Genes, Viral; D006561:Herpes Simplex / Q000382:microbiology; D051379:Mice; D008805:Mice, Inbred A; D010537:Peritoneum / Q000382:microbiology; D018139:Simplexvirus / Q000235:genetics / Q000472:pathogenicity*; D014162:Transfection; D014774:Virulence
D004274:DNA, Recombinant; D004279:DNA, Viral; D004262:DNA Restriction Enzymes; C024406:endodeoxyribonuclease HpaI; D015252:Deoxyribonucleases, Type II Site-Specific