title: The effects of castration and/or methimazole feeding on the pituitary response to temperature extremes by cockerels.
abstract: The pituitary of goitrogen-treated White Leghorn cockerels is smaller in size than control birds and the pituitaries of castrated cockerels is nearly twice the size of control birds. The pituitary cells generated by these treatments may not be functional thyrotrophs or gonadotrophs and may not be able to respond to their usual stimuli. Low ambient temperature is a well-known stimulus to the thyroid gland acting through pituitary TSH. Cyclic-AMP-dependent protein kinase activity levels are used here as an index of cellular activity in the pituitary and thyroid glands. Castrated cockerels with or without methimazole treatment do not have an increased pituitary cAMP-dependent protein kinase activity in cold. Methimazole-treated birds have an exaggerated pituitary protein kinase response to cold stress when compared with controls. Pituitary cAMP-dependent protein kinase activity is paralleled by a similar activity increase in the thyroid gland of methimazole-treated cockerels and no increase in the thyroid of castrated birds. Castrated birds at all temperatures have an elevated thyroid cAMP-dependent protein kinase activity ratio which is interpreted as the result of removal of testosterone inhibition.
mesh_terms: D000818:Animals; D001835:Body Weight; D002369:Castration; D002645:Chickens / Q000502:physiology*; D000242:Cyclic AMP / Q000494:pharmacology; D008297:Male; D008713:Methimazole / Q000494:pharmacology*; D009929:Organ Size; D010902:Pituitary Gland / Q000033:anatomy & histology / Q000187:drug effects / Q000502:physiology*; D011494:Protein Kinases / Q000378:metabolism; D013696:Temperature; D013961:Thyroid Gland / Q000033:anatomy & histology / Q000187:drug effects / Q000502:physiology
chemical_list: D008713:Methimazole; D000242:Cyclic AMP; D011494:Protein Kinases