title: Prospective study of cytotoxic T lymphocyte responses to influenza and antibodies to human T lymphotropic virus-III in homosexual men. Selective loss of an influenza-specific, human leukocyte antigen-restricted cytotoxic T lymphocyte response in human T lymphotropic virus-III positive individuals with symptoms of acquired immunodeficiency syndrome and in a patient with acquired immunodeficiency syndrome.
abstract: Peripheral blood leukocytes (PBL) from 18 homosexual men who did not have acquired immunodeficiency syndrome (AIDS) and from 9 heterosexual men were repetitively tested for their ability to generate HLA self-restricted cytotoxic T lymphocyte responses to influenza virus (flu-self) over a 2-yr period. The sera of the same donors were tested for antibodies to human T lymphotropic virus-III (HTLV-III). Six of the homosexual and none of the heterosexual donors consistently generated weak cytotoxic T lymphocyte responses to flu-self. Seven of the homosexual and none of the heterosexual donors were seropositive for antibodies to HTLV-III. No obvious correlation was detected between weak flu-self cytotoxic T lymphocyte responses and antibodies to HTLV-III. However, one homosexual donor generated no detectable cytotoxic T lymphocyte activity to flu-self, although he was a strong responder to HLA-alloantigens. This donor had an OKT4:OKT8 ratio of 0.4 and was seropositive for HTLV-III antigens; HTLV-III virus was identified in his PBL; and he developed AIDS during the course of this study. A second donor with lymphadenopathy and who was seropositive for HTLV-III antigens exhibited marginal cytotoxic T lymphocyte activity to flu-self which he subsequently lost. PBL from two patients, one with Kaposi's sarcoma and one with generalized lymphadenopathy, were also tested for cytotoxic T lymphocyte responses to flu-self and to alloantigens. Both donors failed to generate cytotoxic T lymphocyte to flu-self, but generated strong cytotoxic T lymphocyte responses to alloantigens. The selective loss of an HLA-restricted cytotoxic T lymphocyte response without loss of HLA alloantigenic cytotoxic T lymphocyte activity may be an important functional immunologic characteristic in the development of AIDS.
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology* / Q000382:microbiology; D000914:Antibodies, Viral / Q000276:immunology*; D003601:Cytotoxicity Tests, Immunologic; D017977:Deltaretrovirus / Q000276:immunology* / Q000302:isolation & purification; D005260:Female; D006680:HLA Antigens / Q000276:immunology; D006716:Homosexuality; D006801:Humans; D009980:Influenza A virus / Q000276:immunology*; D007519:Isoantigens / Q000276:immunology; D008206:Lymphatic Diseases / Q000276:immunology; D008214:Lymphocytes / Q000145:classification; D008297:Male; D011446:Prospective Studies; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D006680:HLA Antigens; D007519:Isoantigens