Kinin release from kininogens by calpains.
During the investigation of inhibitory activity of kininogens toward calpains [EC 3.4.22.17], we found that lysyl-bradykinin was liberated from both high molecular weight (HMW) and low molecular weight (LMW) kininogens by the action of the calpains. The kinin liberation occurred in a limited range of calpain to kininogen molar ratios of 0.5:1 to 8:1, and in that condition calpains were simultaneously inhibited 20 to 80% by kininogens. The maximum level of kinin release from HMW and LMW kininogens by calpain I was about 25% and that by calpain II was 20%. These results suggest that in case of inflammation the kininogens play two physiologically distinct roles by interaction with calpains: to release lysyl-bradykinin and to inhibit proteinase activity of calpains derived from the damaged tissues.
D002154:Calpain / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D007704:Kininogens / Q000378:metabolism*; D007705:Kinins / Q000032:analysis / Q000378:metabolism*; D008970:Molecular Weight; D013997:Time Factors
D007704:Kininogens; D007705:Kinins; D002154:Calpain