Organized packaging of kinetoplast DNA networks.
The kinetoplast DNA (kDNA) of Trypanosoma equiperdum is organized as a complex structure of catenated circular DNA molecules. The major component of the kDNA network is the one kilobase minicircle that is present at about 10,000 copies per network. We have developed two assays to examine the structure of kDNA networks compacted in vitro with spermidine. Our results suggest that minicircles are arranged into a regular structure with an exposed domain which is DNAase I- and restriction-sensitive and a protected domain which is resistant to restriction endonucleases and DNAase I. This regularly packaged structure is dependent upon spermidine compaction and the circularity of the kDNA, but does not require supercoiled minicircles or catenated networks.
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D004262:DNA Restriction Enzymes; D004270:DNA, Circular; D004272:DNA, Mitochondrial; D003850:Deoxyribonuclease I; D008928:Mitochondria / Q000648:ultrastructure; D009690:Nucleic Acid Conformation; D014345:Trypanosoma / Q000648:ultrastructure*
D004270:DNA, Circular; D004272:DNA, Mitochondrial; D004262:DNA Restriction Enzymes; D003850:Deoxyribonuclease I