title: The induction of stress-related proteins by lead.
abstract: Differential inductive effects of lead on protein synthesis in rat fibroblasts and kidney epithelial cells were examined. The lead was administered as lead glutamate, a complex known to introduce lead into cells. Lead exposure induced the synthesis of three proteins which constitute two separate stress protein subgroups. Two of these proteins have been previously identified as the glucose-regulated proteins because their synthesis is induced by reagents which perturb glucose utilization. The third protein is inducible by several sulfhydryl-binding reagents including lead. This third protein has been compared with a protein, p32/6.3, of very similar size and isoelectric point, which has been associated with lead-induced intranuclear inclusion bodies. However, several features, including one-dimensional peptide maps, indicated that the third protein and p32/6.3 are not identical. The three lead-induced proteins are distinguished from the major group of stress proteins by their relative insensitivity to heat stress. Lead glutamate, on the other hand, induces neither the heat shock protein 70 nor metallothionein, both of which are strongly induced by several metals including cadmium, zinc, and mercury.
mesh_terms: D000818:Animals; D002104:Cadmium / Q000494:pharmacology; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000032:analysis / Q000096:biosynthesis*; D002479:Inclusion Bodies / Q000032:analysis; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism; D007854:Lead / Q000494:pharmacology*; D051381:Rats; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D006360:Heat-Shock Proteins; D002104:Cadmium; D007854:Lead