Accumulation of fructose 1,6-bisphosphate in mutant cells of mucoid Pseudomonas aeruginosa as an evidence of phosphofructokinase activity.
Phosphoglucose isomerase negative mutant of mucoid Pseudomonas aeruginosa accumulated relatively higher concentration of fructose 1,6-bisphosphate (Fru-1,6-P2) when mannitol induced cells were incubated with this sugar alcohol. Also the toluene-treated cells of fructose 1,6-bisphosphate aldolase negative mutant of this organism produced Fru-1,6-P2 from fructose 6-phosphate in presence of ATP, but not from 6-phosphogluconate. The results together suggested the presence of an ATP-dependent fructose 6-phosphate kinase (EC 2.7.1.11) in mucoid P. aeruginosa.
D000255:Adenosine Triphosphate / Q000378:metabolism; D005634:Fructose-Bisphosphate Aldolase / Q000378:metabolism; D005635:Fructosediphosphates / Q000378:metabolism*; D005956:Glucose-6-Phosphate Isomerase / Q000378:metabolism; D006598:Hexosediphosphates / Q000378:metabolism*; D008353:Mannitol / Q000378:metabolism; D009154:Mutation; D025481:Phosphofructokinase-2; D010770:Phosphotransferases / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000201:enzymology* / Q000235:genetics
D005635:Fructosediphosphates; D006598:Hexosediphosphates; D008353:Mannitol; D000255:Adenosine Triphosphate; D010770:Phosphotransferases; D025481:Phosphofructokinase-2; D005634:Fructose-Bisphosphate Aldolase; D005956:Glucose-6-Phosphate Isomerase; C029063:fructose-1,6-diphosphate