Nuclear location of all three influenza polymerase proteins and a nuclear signal in polymerase PB2.
In order to re-examine the sub-cellular location of the three influenza A/NT/60/68 polymerase proteins PB1, PB2 and PA in infected cells, specific antisera for each polymerase component have been prepared by immunizing rabbits with polymerase-beta-galactosidase fusion proteins synthesized in Escherichia coli. We show that polymerase PB1, PB2, and PA are predominantly associated with the nucleus of influenza-infected MDCK cells by immunocytochemical techniques. In the case of polymerase PB2 we investigate the possibility that nuclear accumulation is an intrinsic property of the PB2 protein. Using a vaccinia-PB2 recombinant virus, we show that PB2 accumulates intra-nuclearly in monkey CV-1 cells in the absence of any other influenza protein, suggesting it contains an intrinsic nuclear signal.
D000818:Animals; D002460:Cell Line; D002467:Cell Nucleus / Q000201:enzymology*; D002522:Chlorocebus aethiops; D004262:DNA Restriction Enzymes; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay; D007106:Immune Sera; D009980:Influenza A virus / Q000201:enzymology*; D007668:Kidney
D007106:Immune Sera; D012321:DNA-Directed RNA Polymerases; D004262:DNA Restriction Enzymes