A 200 kDa surface glycoprotein of human fibroblasts encoded by a gene on chromosome 11 is regulated by cyclic AMP.
A gene mapped to the long arm of human chromosome 11 was previously characterized to code for a cell-surface glycoprotein of an apparent molecular mass of 200 kDa in fibroblasts. We now report that this surface protein is expressed in an increased amount when human fibroblasts or human X Chinese-hamster hybrid cells containing human chromosome 11 are treated with 1 mM dibutyryl cyclic AMP. A detailed analysis of this phenomenon of induction was performed using a long-established, stable cell line of human X Chinese-hamster hybrid cells, J1 clone, which contained human chromosome 11 and expressed the 200 kDa glycoprotein of human origin. It was shown that the 200 kDa protein of J1 cells was inducible with 1 mM dibutyryl cyclic AMP, 10 mM unmodified cyclic AMP, 0.2 mM 8-para-chlorophenylthio cyclic AMP or 1 nM cholera toxin. Induction was potentiated by the addition of a phosphodiesterase inhibitor, Ro 20-1724. These results are consistent with the finding in human fibroblast, confirming that human fibroblasts express a 200 kDa glycoprotein on their surface which is regulated by intracellular concentrations of cyclic AMP. The regulation may be at the level of transcription since the addition of actinomycin D prevented the induction.
D012368:4-(3-Butoxy-4-methoxybenzyl)-2-imidazolidinone / Q000494:pharmacology; D000494:Allosteric Regulation; D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D002478:Cells, Cultured; D002772:Cholera Toxin / Q000494:pharmacology; D002874:Chromosome Mapping; D002906:Chromosomes, Human, 6-12 and X; D006224:Cricetinae; D003412:Cricetulus; D000242:Cyclic AMP / Q000031:analogs & derivatives / Q000378:metabolism* / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D004790:Enzyme Induction / Q000187:drug effects; D005260:Female; D005347:Fibroblasts / Q000032:analysis; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D006822:Hybrid Cells / Q000032:analysis; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D010053:Ovary; D013873:Thionucleotides / Q000494:pharmacology
D006023:Glycoproteins; D008565:Membrane Proteins; D013873:Thionucleotides; D003609:Dactinomycin; D012368:4-(3-Butoxy-4-methoxybenzyl)-2-imidazolidinone; C015929:8-((4-chlorophenyl)thio)cyclic-3',5'-AMP; D003994:Bucladesine; D002772:Cholera Toxin; D000242:Cyclic AMP