Effect of estrogen on the affinity of malachite green for staining cardiac lipid inclusions in mice.
The effect of estradiol-17-beta on lipids of the ventricular myocardium of mice has been studied with a cytochemical technique in which malachite green was added to glutaraldehyde. This malachite green-glutaraldehyde fixative enhances the visualization of certain phospholipid-related elements. Estrogen induces an affinity of ventricular cardiac lipid inclusions for the cationic dye malachite green. The staining affinity is evidenced only in the estrous female, not in diestrus. In oophorectomized animals, malachite green staining is seen only following estradiol injection, but this effect is blocked by progesterone. In the male, ventricular lipids do not stain, nor do they develop malachite green affinity with estrogen stimulation. These results imply a blockade of the estradiol-mediated dye affinity by progesterone and testosterone. This reinforces the concept of the heart as a target organ for sex steroids and expands the previously described estrogen effects on myocardium.
D000818:Animals; D002369:Castration; D004031:Diestrus; D004958:Estradiol / Q000494:pharmacology*; D004971:Estrus; D005260:Female; D002479:Inclusion Bodies / Q000378:metabolism*; D050356:Lipid Metabolism; D008297:Male; D051379:Mice; D008854:Microscopy, Electron; D009206:Myocardium / Q000378:metabolism* / Q000648:ultrastructure; D012394:Rosaniline Dyes
D012394:Rosaniline Dyes; C005095:malachite green; D004958:Estradiol