Production of acquired immunodeficiency syndrome-associated retrovirus in human and nonhuman cells transfected with an infectious molecular clone.
We constructed an infectious molecular clone of acquired immunodeficiency syndrome-associated retrovirus. Upon transfection, this clone directed the production of infectious virus particles in a wide variety of cells in addition to human T4 cells. The progeny, infectious virions, were synthesized in mouse, mink, monkey, and several human non-T cell lines, indicating the absence of any intracellular obstacle to viral RNA or protein production or assembly. During the course of these studies, a human colon carcinoma cell line, exquisitely sensitive to DNA transfection, was identified.
D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics; D017977:Deltaretrovirus / Q000235:genetics / Q000254:growth & development*; D005786:Gene Expression Regulation; D006801:Humans; D008214:Lymphocytes / Q000382:microbiology; D011991:Receptors, Virus / Q000502:physiology; D013045:Species Specificity; D014162:Transfection; D014779:Virus Replication
D004279:DNA, Viral; D011991:Receptors, Virus