Correlation of polyalbumin receptors with hepatitis B surface antigen polypeptides in human sera.
The molecular nature of the association between hepatitis B surface antigen (HBsAg), hepatitis B e antigen (HBeAg), polymerized human serum albumin receptors (pHSA-R), and HBsAg polypeptides is controversial. Therefore, we studied HBsAg, HBeAg, and pHSA-R by radioimmunoassay in sera of 27 patients with acute or chronic hepatitis B virus infection. In addition, the HBsAg polypeptides were demonstrated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by silver staining and immunoblot analysis. As a group, HBeAg-positive sera contained significantly more HBsAg and higher pHSA-R activity than HBeAg-negative sera. At a given amount of HBsAg, pHSA-R activity was significantly higher in HBeAg-positive sera than in HBeAg-negative sera. However, there was no correlation between the presence or absence of a particular HBsAg polypeptide and pHSA-R activity, suggesting that posttranscriptional events such as glycosylation may modulate pHSA-R activity associated with native HBsAg.
D000208:Acute Disease; D002908:Chronic Disease; D006509:Hepatitis B / Q000097:blood*; D006510:Hepatitis B Antibodies / Q000032:analysis; D006514:Hepatitis B Surface Antigens / Q000032:analysis*; D006513:Hepatitis B e Antigens / Q000032:analysis*; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D007163:Immunosorbent Techniques; D011863:Radioimmunoassay; D017457:Receptors, Albumin; D011956:Receptors, Cell Surface / Q000097:blood*
D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens; D006513:Hepatitis B e Antigens; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D017457:Receptors, Albumin; D011956:Receptors, Cell Surface