Liquid-chromatographic determination of isoenzymes of alkaline phosphatase in serum and tissue homogenates.
We describe a new method for separating alkaline phosphatase (AP) isoenzymes by means of "high-performance" liquid chromatography. Isoenzymes are eluted from the column (Mono Q HR 5/5, a strong anion-exchanger) with a stepwise gradient of LiCl. The isoenzymes originating from small intestine, bone, liver, and bile were identified by use of tissue homogenates, pathological sera, and heat inactivation.
D000469:Alkaline Phosphatase / Q000032:analysis* / Q000097:blood; D002712:Chlorides; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006358:Hot Temperature; D006801:Humans; D007527:Isoenzymes / Q000032:analysis* / Q000097:blood; D008094:Lithium; D018021:Lithium Chloride; D010649:Phenylalanine / Q000494:pharmacology
D002712:Chlorides; D007527:Isoenzymes; D010649:Phenylalanine; D008094:Lithium; D000469:Alkaline Phosphatase; D018021:Lithium Chloride