title: Alteration of alveolar macrophage functions after aerosol infection with bovine herpesvirus type 1.
abstract: Calves were aerosol challenged with bovine herpesvirus type I, and bronchoalveolar cells were subsequently retrieved by lavage from days 1 to 8 postinfection. Alveolar macrophages (AM), which were depleted of contaminating cells, were characterized with respect to phenotypic markers and functional activities. In most aspects, the changes suggested a stimulation of the AM. With variations in kinetics the percentage of AM expressing an MHC II antigen and Fc (immunoglobulin G)-mediated phagocytosis increased, as did the activity level of two ectoenzymes and the lysosomal hydrolase beta-glucoronidase. The generation of prostaglandin E2 by the AM also rose significantly. However, selective suppression of cellular cytotoxicity and interleukin-1 generation was observed. These findings may have important implications for understanding the events involved in the virus-bacterial interaction in respiratory diseases.
mesh_terms: D015720:5'-Nucleotidase; D000336:Aerosols; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D000911:Antibodies, Monoclonal; D000920:Antibody-Dependent Cell Cytotoxicity; D000954:Antigens, Surface / Q000032:analysis; D043182:Carboxylesterase; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism; D002417:Cattle; D003602:Cytotoxicity, Immunologic; D007242:Herpesvirus 1, Bovine / Q000276:immunology*; D007241:Infectious Bovine Rhinotracheitis / Q000276:immunology*; D007375:Interleukin-1 / Q000378:metabolism; D007931:Leucyl Aminopeptidase / Q000378:metabolism; D008214:Lymphocytes / Q000276:immunology; D008264:Macrophages / Q000276:immunology*; D009504:Neutrophils / Q000276:immunology; D009708:Nucleotidases / Q000378:metabolism; D010587:Phagocytosis
chemical_list: D000336:Aerosols; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D007375:Interleukin-1; D002265:Carboxylic Ester Hydrolases; D043182:Carboxylesterase; D009708:Nucleotidases; D000469:Alkaline Phosphatase; D015720:5'-Nucleotidase; D007931:Leucyl Aminopeptidase