Specific binding of the mononuclear phagocyte colony-stimulating factor CSF-1 to the product of the v-fms oncogene.
Cells transformed by the McDonough strain of feline sarcoma virus (SM-FeSV) express a v-fms-encoded glycoprotein whose expression at the cell surface correlates with the transformed phenotype. The mouse mononuclear phagocyte growth factor CSF-1 specifically binds to SM-FeSV-transformed cells at high-affinity sites indistinguishable from those detected on normal feline macrophages. A monoclonal antibody to a v-fms-encoded epitope competed for CSF-1 binding to SM-FeSV-transformed cells, and chemical crosslinking demonstrated that murine CSF-1 bound to the v-fms gene product at the cell surface. Although SM-FeSV-transformed fibroblast lines were found to secrete CSF-1, the growth of transformed cells was not affected by antibodies to the v-fms gene product or to the growth factor. Tyrosine phosphorylation of the v-fms products in membranes was observed in the absence of CSF-1 and was not enhanced by addition of the murine growth factor. The data support the hypothesis that the c-fms protooncogene product is related, and possibly identical, to the CSF-1 receptor and suggest that the v-fms-encoded kinase functions in the absence of an exogenous growth factor.
D000818:Animals; D002415:Cats; D002471:Cell Transformation, Neoplastic / Q000473:pathology; D002472:Cell Transformation, Viral; D003115:Colony-Stimulating Factors / Q000378:metabolism*; D006133:Growth Substances / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism; D051379:Mice; D008907:Mink; D008970:Molecular Weight; D009000:Monocytes / Q000378:metabolism*; D009856:Oncogene Proteins, Viral / Q000378:metabolism*; D010750:Phosphoproteins / Q000378:metabolism; D010766:Phosphorylation; D051381:Rats; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism*; D016184:Receptors, Colony-Stimulating Factor; D005253:Sarcoma Viruses, Feline
D003115:Colony-Stimulating Factors; D006133:Growth Substances; D009856:Oncogene Proteins, Viral; D010750:Phosphoproteins; D011956:Receptors, Cell Surface; D016184:Receptors, Colony-Stimulating Factor