Isolation and amino acid sequence of the smallest subunit of beef heart bc1 complex.
The 11 subunits of beef heart bc1 complex can be separated either by a new SDS-PAGE system or by a series of chromatographic steps involving dissociation of the complex by salt treatment. The amino acid sequence of the smallest subunit was determined by complete solid-phase Edman degradation and was confirmed by sequencing the N-terminal part and the C-terminal tryptic fragment by liquid-phase Edman degradation. The protein consists of 56 amino acid residues; the Mr was calculated to be 6363. The protein ('ISP binding factor') might be entangled in the reassembly of the iron-sulfur protein with the bc1 subcomplex.
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002845:Chromatography / Q000379:methods; D014450:Electron Transport Complex III; D004591:Electrophoresis, Polyacrylamide Gel; D009097:Multienzyme Complexes / Q000302:isolation & purification*; D009206:Myocardium / Q000201:enzymology*; D010446:Peptide Fragments / Q000302:isolation & purification; D011487:Protein Conformation; D011808:Quinone Reductases / Q000302:isolation & purification*; D012492:Salts
D009097:Multienzyme Complexes; D010446:Peptide Fragments; D012492:Salts; D011808:Quinone Reductases; D014450:Electron Transport Complex III