Separation of [1-3H]cellooligosaccharides by thin-layer chromatography: assay for cellulolytic enzymes.
A thin-layer chromatographic method for the separation with high resolution of [1-3H]cellooligosaccharides on silica gel plates has been developed. Reducing end-labeled glucose through cellohexaose were separated on silica gel plates with three ascents of ethyl acetate:water: methanol (40:15:20; v:v) and each was extracted with an efficiency of 88 +/- 3%. Separations of cellooligosaccharides using other adsorbents, solvents, and impregnants are also described. This thin-layer chromatographic method facilitated analysis of the activity of cellulolytic enzymes.
D002482:Cellulose / Q000378:metabolism*; D043366:Cellulose 1,4-beta-Cellobiosidase; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D005782:Gels; D006026:Glycoside Hydrolases / Q000032:analysis*; D006868:Hydrolysis; D007700:Kinetics; D009844:Oligosaccharides / Q000302:isolation & purification*; D012822:Silicon Dioxide; D012997:Solvents
D005782:Gels; D009844:Oligosaccharides; D012997:Solvents; D012822:Silicon Dioxide; D002482:Cellulose; D006026:Glycoside Hydrolases; D043366:Cellulose 1,4-beta-Cellobiosidase