Cloning of cDNA encoding the murine IgG1 induction factor by a novel strategy using SP6 promoter.
Complementary DNA encoding the IgG1 induction factor, the first lymphokine directed to B lymphocytes, from a murine T-cell line has been cloned using a new strategy. The putative primary amino-acid sequence was deduced from the nucleotide sequence determined. The lymphokine synthesized by the direction of this cloned cDNA has many other functions, such as production of B-cell growth factor-1 and induction of Ia on B cells.
D000595:Amino Acid Sequence; D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004262:DNA Restriction Enzymes; D005822:Genetic Vectors; D006133:Growth Substances / Q000235:genetics*; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D007074:Immunoglobulin G / Q000096:biosynthesis; D015847:Interleukin-4; D008222:Lymphokines / Q000235:genetics*; D051379:Mice; D011401:Promoter Regions, Genetic; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics; D013601:T-Lymphocytes / Q000502:physiology*; D014982:Xenopus laevis
D006133:Growth Substances; D000949:Histocompatibility Antigens Class II; D007074:Immunoglobulin G; D008222:Lymphokines; D012333:RNA, Messenger; D015847:Interleukin-4; D004247:DNA; D004262:DNA Restriction Enzymes