title: Characterization of a herpes simplex virus type 2-specified glycoprotein with affinity for N-acetylgalactosamine-specific lectins and its identification as g92K or gG.
abstract: Extracts from herpes simplex virus type 2 (HSV-2)-infected cells were subjected to affinity chromatography with gel-bound Helix pomatia lectin (HPA). Only one HSV-2-specified glycoprotein was isolated by this procedure and the glycoprotein had an apparent molecular weight of 130 000 (130K). The HPA-binding glycoprotein was genetically mapped, using HSV-1 X HSV-2 intertypic recombinants into the short component of the HSV-2 genome. The mapping position, electrophoretic mobility and the antigenic properties of the HPA-binding protein indicated that it was unrelated to glycoprotein C (gC), which is the HPA-binding glycoprotein in HSV-1-infected cells, and distinct from gE and gD which map in the S component. The glycoprotein was almost quantitatively precipitated by monoclonal antibody AP1, specific for glycoprotein g92K and it also reacted with monoclonal antibody 1206-3, specific for the HSV-2 glycoprotein G previously described. It is concluded that the isolated glycoprotein is identical to g92K and consequently also to the HSV-2-specific glycoprotein G.
mesh_terms: D000116:Acetylgalactosamine / Q000032:analysis; D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology; D002846:Chromatography, Affinity; D005814:Genes, Viral; D037102:Lectins; D008970:Molecular Weight; D018139:Simplexvirus / Q000032:analysis* / Q000235:genetics; D014759:Viral Envelope Proteins / Q000032:analysis* / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; C038095:Helix lectin; D037102:Lectins; D014759:Viral Envelope Proteins; C045464:glycoprotein G, herpes simplex virus type 2; D000116:Acetylgalactosamine