Enzymatic purification of chemically synthesized oligodeoxyribonucleotides prior to removal from a solid-support.
A novel combined solid-phase DNA synthesis and solid-phase DNA purification strategy is described. Removal of all N-, cap-, and phosphate protecting groups while maintaining a 5'-blocking group on the final product followed by a 5'- specific exonuclease digestion of failure sequences with the synthetic DNA still attached to the solid phase yields an essentially pure oligomer.
D004247:DNA / Q000138:chemical synthesis; D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis* / Q000302:isolation & purification; D043264:Phosphodiesterase I; D010727:Phosphoric Diester Hydrolases
D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides; D004247:DNA; D010727:Phosphoric Diester Hydrolases; D043264:Phosphodiesterase I