Immunization against bovine papillomavirus infection.
The two large open reading frames denoted L1 and L2 in the non-transforming region of the bovine papillomavirus type 1 (BPV-1) genome have been molecularly cloned to expression in Escherichia coli. Antisera against the E. coli-derived L1 and L2 protein reacted with BPV-1 in both enzyme-linked immunosorbent assays and immunoprecipitation reactions. Neutralization of BPV-induced transformation of mouse C127 cells was demonstrated most consistently with antisera against the L1 protein. E. coli-derived L1 protein protected calves against BPV-1 challenge after vaccination.
D000818:Animals; D001345:Autoradiography; D052688:Bovine papillomavirus 1 / Q000235:genetics / Q000276:immunology*; D002417:Cattle; D002418:Cattle Diseases / Q000517:prevention & control*; D003001:Cloning, Molecular; D004274:DNA, Recombinant; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000235:genetics; D007163:Immunosorbent Techniques; D027383:Papillomaviridae / Q000276:immunology*; D010957:Plasmids; D014412:Tumor Virus Infections / Q000517:prevention & control / Q000662:veterinary*; D014611:Vaccination / Q000662:veterinary; D014765:Viral Vaccines; D014860:Warts / Q000517:prevention & control / Q000662:veterinary*
D004274:DNA, Recombinant; D014765:Viral Vaccines