title: Repopulation of Leydig cells in mature rats after selective destruction of the existent Leydig cells with ethylene dimethane sulfonate is dependent on luteinizing hormone and not follicle-stimulating hormone.
abstract: After selective destruction of Leydig cells in mature rats with ethylene dimethane sulfonate (EDS), repopulation of Leydig cells occurs. This repopulation process was studied in normal and sterile (prenatally irradiated) rats using morphological and histochemical techniques and by measuring hormone concentrations. Three days after administration of EDS to normal rats, extensive Leydig cell degeneration had occurred, testosterone concentrations were decreased to less than 10% of the normal value, and no 3 beta-hydroxysteroid dehydrogenase activity or pregnenolone production could be detected in isolated interstitial cells. Seven days after EDS administration, no cells with the appearance of Leydig cells were observed, and steroidogenic activities were still absent. After 14 days, single or paired Leydig cells were present again in the interstitium, but only after 21 days an increase in the plasma testosterone concentration and LH-dependent pregnenolone production was observed. On day 35, numerous Leydig cells were present, and testosterone levels were restored to normal. The depletion and repopulation of Leydig cells after administration of EDS to sterile rats showed a somewhat different pattern. Three days after administration of EDS, testosterone concentrations were decreased to less than 10% of the normal value, and isolated interstitial cells showed no steroidogenic activities as in normal rats, but a small number of Leydig cells was still present. A similar picture was observed between 4 and 9 days after EDS administration. This indicates that some Leydig cells from sterile rats, unlike Leydig cells from normal rats, were resistant to EDS. The repopulation of Leydig cells in sterile rats was faster than in normal rats. After 14 days, many groups of Leydig cells were present in the interstitium, and the plasma testosterone concentration and pregnenolone production in vitro were significantly increased. Normal plasma testosterone levels were restored on day 21. Serum LH and FSH were decreased immediately after EDS administration, but during the next days a sharp rise was observed in both normal and sterile rats. The rise in LH correlated with the decrease in testosterone, and restoration of LH levels took place when testosterone levels increased. FSH levels changed similarly, but were delayed, in comparison to LH. In rats with testosterone implants that suppressed LH levels to less than 2 ng/ml and maintained normal FSH levels, ranging from 150-340 ng/ml, as well as in hypophysectomized rats, no repopulation of Leydig cells could be observed until 35 days after EDS treatment.(ABSTRACT TRUNCATED AT 400 WORDS)
mesh_terms: D015096:3-Hydroxysteroid Dehydrogenases / Q000378:metabolism; D000818:Animals; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D005640:Follicle Stimulating Hormone / Q000097:blood / Q000494:pharmacology*; D007016:Hypophysectomy; D007248:Infertility, Male / Q000378:metabolism / Q000473:pathology*; D007700:Kinetics; D007985:Leydig Cells / Q000187:drug effects / Q000473:pathology*; D007986:Luteinizing Hormone / Q000097:blood / Q000494:pharmacology*; D008297:Male; D008698:Mesylates / Q000494:pharmacology*; D011284:Pregnenolone / Q000096:biosynthesis; D051381:Rats; D011919:Rats, Inbred Strains; D013739:Testosterone / Q000097:blood
chemical_list: D006063:Chorionic Gonadotropin; D008698:Mesylates; D013739:Testosterone; D011284:Pregnenolone; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D015096:3-Hydroxysteroid Dehydrogenases; C002994:ethylene dimethanesulfonate