Chlorpromazine induces accumulation of inositol phosphates in C6 glioma cells.
The capacity to modify the incorporation of [2-3H]myo-inositol into inositides and inositol phosphates was different for three psychotropic cationic amphiphilic drugs. Chlorpromazine, desmethylimipramine and propranolol were able to increase the labeling of inositol-containing lipids, but only chlorpromazine dramatically increased the incorporation into inositol phosphate, -bisphosphate and -trisphosphate. The increase was 10- to 50-fold in 60 min as compared with controls. This effect is not due to stimulation of lipid labeling, because in chase experiments radioactivity in inositol phosphates increased to a greater extent than in their parent lipids. It is possible that the alteration of phosphoinositide catabolism is related to the neuroleptic activity of the drug.
D000818:Animals; D002460:Cell Line; D002746:Chlorpromazine / Q000494:pharmacology*; D003891:Desipramine / Q000494:pharmacology; D005910:Glioma / Q000378:metabolism; D007294:Inositol / Q000378:metabolism; D007295:Inositol Phosphates / Q000378:metabolism*; D008246:Lysophospholipids; D010716:Phosphatidylinositols / Q000378:metabolism; D011433:Propranolol / Q000494:pharmacology; D051381:Rats; D013403:Sugar Phosphates / Q000378:metabolism*
D007295:Inositol Phosphates; D008246:Lysophospholipids; D010716:Phosphatidylinositols; D013403:Sugar Phosphates; C025449:lysophosphatidylinositol; C014575:glycerylphosphoinositol; D007294:Inositol; D011433:Propranolol; D003891:Desipramine; D002746:Chlorpromazine