Madin-Darby canine kidney cells display type I and type II insulin-like growth factor (IGF) receptors.
Using affinity cross-linking techniques, we report the presence of type I IGF and type II IGF receptors in Madin-Darby canine kidney cells, a line of cells lacking insulin receptors. The IGF receptors were further characterized by competition binding studies and found to be similar to IGF receptors in other tissue types. In Madin-Darby canine kidney cells, the type I IGF receptor binds IGF-I greater than IGF-II greater than insulin and the type II IGF receptor binds IGF-II and IGF-I with approximately the same affinity, but does not bind insulin.
D000818:Animals; D001667:Binding, Competitive; D002460:Cell Line; D004285:Dogs; D007328:Insulin / Q000378:metabolism; D007334:Insulin-Like Growth Factor I / Q000378:metabolism; D007668:Kidney / Q000032:analysis* / Q000166:cytology; D008970:Molecular Weight; D011956:Receptors, Cell Surface / Q000032:analysis*; D017451:Receptors, Somatomedin
D007328:Insulin; D011956:Receptors, Cell Surface; D017451:Receptors, Somatomedin; D007334:Insulin-Like Growth Factor I