Mapping of a second recombination hot spot within the I-E region of the mouse H-2 gene complex.
The crossover points of nine intra-I region recombinant mouse strains were determined by restriction fragment analysis. The recombinants were examined for the presence of k and p haplotype specific DNA restriction endonuclease sites. These restriction sites were a Sac I site between the E beta and E beta 2 genes, a Hpa I site within the E beta 2 gene, and a Rsa I site approximately 1 kb to the right of the E alpha gene. Seven of the recombinants were found to have crossovers between the Hpa I and the Rsa I site. This analysis suggests that a recombination hot spot exists within this segment. This segment is approximately 12-14 kb, and contains the E alpha gene and the intervening sequence between the E beta 2 and E alpha genes.
D000818:Animals; D004247:DNA / Q000032:analysis; D004262:DNA Restriction Enzymes; D006183:H-2 Antigens / Q000235:genetics; D006649:Histocompatibility Antigens / Q000235:genetics*; D000949:Histocompatibility Antigens Class II; D008285:Major Histocompatibility Complex; D051379:Mice; D009838:Oligodeoxyribonucleotides / Q000032:analysis; D011110:Polymorphism, Genetic; D011995:Recombination, Genetic
D006183:H-2 Antigens; D006649:Histocompatibility Antigens; D000949:Histocompatibility Antigens Class II; C024138:I-E-antigen; D009838:Oligodeoxyribonucleotides; D004247:DNA; D004262:DNA Restriction Enzymes