Partial purification of a nucleoside triphosphatase from the inner membrane of the chloroplast envelope of pea.
A Mg2+-NTPase has been partially purified from the inner membrane of the pea chloroplast envelope. Isolated envelope membranes were solubilized with Triton X-100 and fractionated by DEAE-Sephadex chromatography, followed by ultrafiltration and sucrose density gradient centrifugation. An approximate 35-fold increase in the specific activity of the vanadate and sodium fluoride sensitive NTPase was obtained. Analysis of the partially purified NTPase by sodium dodecyl sulfate polyacrylamide gel electrophoresis revealed a single 37-kDa polypeptide that appeared to be associated with the activity. In support of this identification, it is demonstrated that the 37-kDa polypeptide can be photolabeled with 8-azido-ATP.
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000031:analogs & derivatives / Q000494:pharmacology; D000345:Affinity Labels; D001386:Azides / Q000494:pharmacology; D002499:Centrifugation, Density Gradient; D002736:Chloroplasts / Q000201:enzymology*; D002852:Chromatography, Ion Exchange; D003902:Detergents; D007887:Fabaceae; D007425:Intracellular Membranes / Q000201:enzymology; D043583:Nucleoside-Triphosphatase; D010744:Phosphoric Monoester Hydrolases / Q000037:antagonists & inhibitors / Q000302:isolation & purification*; D010777:Photochemistry; D010946:Plants, Medicinal; D012995:Solubility; D014639:Vanadium / Q000494:pharmacology
D000345:Affinity Labels; D001386:Azides; D003902:Detergents; D014639:Vanadium; C017040:8-azidoadenosine 5'-triphosphate; D000255:Adenosine Triphosphate; D010744:Phosphoric Monoester Hydrolases; D000251:Adenosine Triphosphatases; D043583:Nucleoside-Triphosphatase