title: Methods for the detection and quantitation of angiotensin converting enzyme inhibitors in fermentation broths.
abstract: Two procedures are described for the detection of inhibitors of angiotensin converting enzyme (ACE). The first is a new agar-plate method useful as a screening tool. Two ACE inhibitors produced by culture A58365 were discovered using this plate test. The second method is a modification of a previously reported spectrophotometric procedure. Both procedures utilize p-nitrobenzyl-oxycarbonylglycyl-(S-4-nitrobenzo-2-oxa-1,3- diazole)-L-cysteinyl-glycine as substrate.
mesh_terms: D000806:Angiotensin-Converting Enzyme Inhibitors; D000818:Animals; D004791:Enzyme Inhibitors / Q000032:analysis; D005285:Fermentation; D011817:Rabbits; D013053:Spectrophotometry
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D004791:Enzyme Inhibitors