Derivatives of Co(II), Co(III), Ni(II), Cu(II), and Zn(II) with 5' AMP. Use as enzymatic labels with glycogen phosphorylase B.
Some new derivatives of Co(II), Co(III), Ni(II), Cu(II), and Zn(II) with 5' AMP have been obtained, characterized by elemental analysis, infrared, electronic, and fluorescence spectroscopy. The activities of these complexes as substitutes of 5' AMP as allosteric activators of glycogen phosphorylase b have been tested. The derivatives that have no interaction with the phosphate group are good analogs of the natural allosteric activator; the complexes that have direct bonding between metallic ion and phosphate groups do not activate the enzyme.
D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D003035:Cobalt / Q000032:analysis; D003300:Copper / Q000032:analysis; D000242:Cyclic AMP / Q000031:analogs & derivatives*; D004121:Dimethyl Sulfoxide; D008670:Metals / Q000032:analysis*; D009132:Muscles / Q000201:enzymology; D009532:Nickel / Q000032:analysis; D010763:Phosphorylase b / Q000378:metabolism*; D006005:Phosphorylases / Q000378:metabolism*; D011817:Rabbits; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D015032:Zinc / Q000032:analysis
D008670:Metals; D003035:Cobalt; D003300:Copper; D009532:Nickel; D000242:Cyclic AMP; D010763:Phosphorylase b; D006005:Phosphorylases; D015032:Zinc; D004121:Dimethyl Sulfoxide