Inhibition of polyphosphoinositide phosphodiesterase by aminoglycoside antibiotics.
The calcium-activated phosphodiesteratic hydrolysis of 32P-labeled phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol 4-phosphate in prelabeled nerve ending membranes is inhibited by the aminoglycosides neomycin and gentamicin, and to a lesser extent, by streptomycin. The inhibition is overcome by increasing concentrations of Ca2+, indicating that the aminoglycosides exert their effect by displacing Ca2+ from lipid.
D000617:Aminoglycosides / Q000494:pharmacology; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism / Q000494:pharmacology; D005839:Gentamicins / Q000494:pharmacology; D066298:In Vitro Techniques; D008566:Membranes / Q000187:drug effects / Q000378:metabolism; D009355:Neomycin / Q000494:pharmacology; D009411:Nerve Endings / Q000187:drug effects / Q000378:metabolism; D019269:Phosphatidylinositol 4,5-Diphosphate; D018129:Phosphatidylinositol Phosphates; D010716:Phosphatidylinositols / Q000378:metabolism; D010726:Phosphodiesterase Inhibitors; D054801:Phosphoinositide Phospholipase C; D010727:Phosphoric Diester Hydrolases; D013307:Streptomycin / Q000494:pharmacology
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D005839:Gentamicins; D019269:Phosphatidylinositol 4,5-Diphosphate; D018129:Phosphatidylinositol Phosphates; D010716:Phosphatidylinositols; D010726:Phosphodiesterase Inhibitors; C037178:phosphatidylinositol 4-phosphate; D010727:Phosphoric Diester Hydrolases; D054801:Phosphoinositide Phospholipase C; D009355:Neomycin; D002118:Calcium; D013307:Streptomycin