The generation of superoxide anions by polymorphonuclear leucocytes from patients with ankylosing spondylitis in response to the stimulant f-met-leu-phe.
Superoxide anion (O2-) generation in response to the chemoattractant f-met-leu-phe was measured in PMNL from 30 patients with ankylosing spondylitis and in PMNL from 27 controls. At a concentration of stimulant above that necessary to achieve maximal response, no differences in O2- production between the groups were noted. With a lower concentration of stimulant, cells from patients with AS or RA taking non-steroidal anti-inflammatory drugs had lower levels of O2- generation, compared both to controls and to AS patients, off medication. Results suggest any decrease in O2- generation seen in patients with AS is likely to be related to therapy and not to the disease or to possession of HLA B27.
D000838:Anions / Q000096:biosynthesis; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use; D001172:Arthritis, Rheumatoid / Q000188:drug therapy / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005260:Female; D006680:HLA Antigens / Q000032:analysis; D015796:HLA-B27 Antigen; D006801:Humans; D008297:Male; D009240:N-Formylmethionine Leucyl-Phenylalanine; D009504:Neutrophils / Q000378:metabolism*; D009994:Osmolar Concentration; D013167:Spondylitis, Ankylosing / Q000188:drug therapy / Q000276:immunology / Q000378:metabolism*; D013268:Stimulation, Chemical; D013481:Superoxides / Q000096:biosynthesis*
D000838:Anions; D000893:Anti-Inflammatory Agents; D006680:HLA Antigens; D015796:HLA-B27 Antigen; D013481:Superoxides; D009240:N-Formylmethionine Leucyl-Phenylalanine