Reversible binding of polymyxin B and neomycin to the solid part of faeces.
Previous studies have shown that polymyxin B and neomycin were bound to the solid part of human faeces. In the present study, the faecal binding of polymyxin B and neomycin was studied, using a wide range of concentrations of drugs and faecal dilutions. Up to 25 mg of polymyxin B and 15 mg of neomycin could bind to 1 g of faeces but the binding was found to be reversible. With faeces of rats it was found that polymyxin B was mainly bound to the non-bacterial part of faeces, whereas neomycin was bound to the bacterial as well as the non-bacterial part. The implications of the reversible binding of antibiotics to intestinal contents for selective decontamination are discussed.
D000818:Animals; D001419:Bacteria / Q000187:drug effects; D005243:Feces / Q000032:analysis* / Q000382:microbiology; D006801:Humans; D008826:Microbial Sensitivity Tests; D009355:Neomycin / Q000378:metabolism* / Q000494:pharmacology; D011112:Polymyxin B / Q000378:metabolism* / Q000494:pharmacology; D011113:Polymyxins / Q000378:metabolism*; D051381:Rats; D013045:Species Specificity
D011113:Polymyxins; D009355:Neomycin; D011112:Polymyxin B