Activation of chicken liver fructose- 1,6-bisphosphatase by oxidized glutathione.
Treatment of chicken liver fructose- 1,6-bisphosphatase with oxidized glutathione (GSSG) leads to an increase in activity. This activation is markedly enhanced if treatment is performed in the presence of AMP or Mn2+. The effects of AMP and Mn2+ appear to be synergistic. The maximal activation is over 13-fold and is accompanied by the disappearance of 4 sulfhydryl groups per molecule of enzyme. Both fructose 1,6-bisphosphate and fructose 2,6-bisphosphate can largely prevent this activation. Activation can be reversed by dithiothreitol or cysteine. It appears that GSSG activates this enzyme by thiol/disulfide exchanges with the enzyme's specific sulfhydryl groups.
D000249:Adenosine Monophosphate / Q000494:pharmacology; D000818:Animals; D002645:Chickens; D004228:Dithionitrobenzoic Acid / Q000494:pharmacology; D004789:Enzyme Activation; D006597:Fructose-Bisphosphatase / Q000378:metabolism*; D005635:Fructosediphosphates / Q000494:pharmacology; D005978:Glutathione / Q000032:analysis / Q000494:pharmacology*; D008099:Liver / Q000201:enzymology*; D008274:Magnesium / Q000494:pharmacology; D008345:Manganese / Q000494:pharmacology; D010084:Oxidation-Reduction
D005635:Fructosediphosphates; D000249:Adenosine Monophosphate; D008345:Manganese; C027652:fructose 2,6-diphosphate; D004228:Dithionitrobenzoic Acid; D006597:Fructose-Bisphosphatase; D005978:Glutathione; D008274:Magnesium; C029063:fructose-1,6-diphosphate