title: Role of DNA topology in Mu transposition: mechanism of sensing the relative orientation of two DNA segments.
abstract: DNA strand transfer at the initiation of Mu transposition normally requires a negatively supercoiled transposon donor molecule, containing both ends of Mu in inverted repeat orientation. We propose that the specific relative orientation of the Mu ends is needed only to energetically favor a particular configuration that the ends must adopt in a synaptic complex. The model was tested by constructing special donor DNA substrates that, because of their catenation or knotting, energetically favor this same configuration of the Mu ends, even though they are on separate molecules or in direct repeat orientation. These structures are efficient substrates for the strand transfer reaction, whereas appropriate control structures are not. The result eliminates tracking or protein scaffold models for orientation preference. Several other systems in which the relative orientation of two DNA segments is sensed may utilize the same mechanism.
mesh_terms: D010583:Bacteriophage mu / Q000235:genetics*; D004251:DNA Transposable Elements; D004278:DNA, Superhelical; D004279:DNA, Viral / Q000235:genetics*; D008957:Models, Genetic; D009690:Nucleic Acid Conformation; D011995:Recombination, Genetic; D014764:Viral Proteins / Q000378:metabolism
chemical_list: D004251:DNA Transposable Elements; D004278:DNA, Superhelical; D004279:DNA, Viral; D014764:Viral Proteins