Cleavage maps of human cytomegalovirus genome (strain Towne) determined by the use of cosmid cloning system.
Virion DNA of human cytomegalovirus strain Towne was partially digested with endonuclease Hind III and fragments larger than 29 kbp were ligated to cosmid pHC79. The whole viral DNA sequence has been cloned in large overlapping segments carried by 32 recombinant cosmid clones (a pIT series). A whole set of Hind III fragments has also been cloned (a pHI series). By using these, we have constructed corrected cleavage maps of strain Towne DNA for Hind III, Bam H I, EcoR I and Xba I.
D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003360:Cosmids; D003587:Cytomegalovirus / Q000235:genetics*; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000235:genetics*
D004279:DNA, Viral; D004262:DNA Restriction Enzymes