Metabolism of gammalinolenic acid by human blood platelet microsomes.
The microsomal fraction was used to test the ability of human platelets to metabolize gammalinolenic acid. The microsomal delta 6 and delta 5 fatty acid desaturase activities were measured and the incorporation of [14C]malonyl CoA into prostaglandins was also determined. The results indicate that human platelets have the capacity to elongate gammalinolenic acid (18:3 n-6) to dihomogammalinolenic acid (20:3 n-6) precursor of PGE1. Labeled PGE1 could be detected when human platelets microsomes were incubated with [14C]malonyl CoA in the presence of gammalinolenic acid. The results also show that human platelet microsomes have little delta 6 or delta 5 desaturase enzyme activity.
D015126:8,11,14-Eicosatrienoic Acid / Q000378:metabolism; D001095:Arachidonic Acids / Q000378:metabolism; D001792:Blood Platelets / Q000378:metabolism*; D002849:Chromatography, Gas; D044943:Fatty Acid Desaturases / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D008042:Linolenic Acids / Q000378:metabolism*; D008861:Microsomes / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism; D011453:Prostaglandins / Q000096:biosynthesis; D017965:gamma-Linolenic Acid
D001095:Arachidonic Acids; D008042:Linolenic Acids; D010743:Phospholipids; D011453:Prostaglandins; D017965:gamma-Linolenic Acid; D044943:Fatty Acid Desaturases; D015126:8,11,14-Eicosatrienoic Acid