title: Evidence for cAMP-mediated control of isoleucyl-tRNA synthetase formation in Escherichia coli K-12.
abstract: The ability of cAMP to inhibit isoleucyl-tRNA synthetase (IRS) formation has been demonstrated in wild type K-12 Escherichia coli and two adenyl-cyclase (cya) mutants. cAMP appeared not to have any effect on either the valyl- or arginyl-tRNA synthetase (VRS and ARS respectively). Addition of cAMP led to a reduction in rate of IRS synthesis but not VRS or ARS. Furthermore, derepression of IRS and VRS by isoleucine limitation was completely prevented by cAMP.
mesh_terms: D000604:Amino Acyl-tRNA Synthetases / Q000096:biosynthesis*; D000242:Cyclic AMP / Q000494:pharmacology*; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development / Q000502:physiology; D005786:Gene Expression Regulation; D007532:Isoleucine / Q000494:pharmacology; D007533:Isoleucine-tRNA Ligase / Q000096:biosynthesis* / Q000235:genetics; D009154:Mutation
chemical_list: D007532:Isoleucine; D000242:Cyclic AMP; D000604:Amino Acyl-tRNA Synthetases; D007533:Isoleucine-tRNA Ligase