Sequence-specific BamHI endonuclease. The proposed role of arginine residues in substrate binding and recognition.
Arginyl residues in BamHI endonuclease were examined because of their alleged role in proteins that contain nucleotide- or phosphate-binding sites. Butanedione, an arginine-specific reagent, inhibited the endonuclease in the presence of sodium borate. The inhibition was decreased by preliminary incubation of the enzyme with DNA or competitive inhibitors which were the 5'-phosphoryl deoxydinucleotide subsets of the BamHI recognition sequence. The dinucleotide pdGpdG protected the enzyme most efficiently against the butanedione modification. Dinucleotides that were unrelated to the recognition sequence failed to protect the enzyme from inactivation. These studies indicate that arginine residues may reside in the enzyme's active site and might function in the sequence-specific recognition of the BamHI palindrome.
D001120:Arginine; D001483:Base Sequence; D001665:Binding Sites; D001881:Borates / Q000494:pharmacology; D004262:DNA Restriction Enzymes / Q000037:antagonists & inhibitors / Q000378:metabolism*; D004269:DNA, Bacterial / Q000378:metabolism / Q000494:pharmacology; D004279:DNA, Viral / Q000378:metabolism / Q000494:pharmacology; D015245:Deoxyribonuclease BamHI; D004852:Epoxy Compounds / Q000494:pharmacology; D009838:Oligodeoxyribonucleotides / Q000494:pharmacology; D013539:Simian virus 40; D013329:Structure-Activity Relationship; D013379:Substrate Specificity
D001881:Borates; D004269:DNA, Bacterial; D004279:DNA, Viral; D004852:Epoxy Compounds; D009838:Oligodeoxyribonucleotides; C007366:diepoxybutane; C010634:sodium borate; D001120:Arginine; D004262:DNA Restriction Enzymes; D015245:Deoxyribonuclease BamHI