H2O2-modification of Na,K-ATPase. Alterations in external Na+ and K+ stimulation of K+ influx.
Studies, at steady state, of the Na,K-ATPase dependent influx of K+ into bovine lenses in organ culture are used to characterize further the H2O2-modification of the Na+ pump. Control lenses display constants for interaction with external Na+ and K+ similar to those obtained for the erythrocyte. H2O2 treatment of the bovine lens leads to total loss of external Na+ stimulation and alteration of external K+ stimulation.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001692:Biological Transport; D002417:Cattle; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D006868:Hydrolysis; D007700:Kinetics; D007908:Lens, Crystalline / Q000201:enzymology* / Q000378:metabolism; D009924:Organ Culture Techniques; D011188:Potassium / Q000378:metabolism / Q000494:pharmacology*; D012964:Sodium / Q000494:pharmacology*; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*
D000255:Adenosine Triphosphate; D012964:Sodium; D006861:Hydrogen Peroxide; D000254:Sodium-Potassium-Exchanging ATPase; D011188:Potassium