Cytological and ultrastructural changes in the pituitary pars distalis of the goldfish kept in calcium-free environments.
The cytology and ultrastructure of the pars distalis, mainly that of prolactin (PRL) cells, were investigated in goldfish adapted to fresh water (FW) or deionized water (DW) for 20 and 40 days, or gradually adapted to 1/3 artificial sea water (ASW) or 1/3 Ca-free sea water. When compared to PRL cells of goldfish kept in FW, those of goldfish adapted to DW did not show signs of increased activity. The lack of exocytotic activity and the low development of various organelles suggested that cell activity was slightly reduced. In 1/3 ASW, PRL cells were smaller and less active. In 1/3 Ca-free ASW, PRL cells appeared slightly stimulated compared with those of fish in 1/3 ASW. The Golgi area was more developed and a few lamellae of endoplasmic reticulum were observed in some cell islets. However, there was no significant difference between PRL cells of goldfish kept in 1/3 Ca-free ASW and in FW. In 1/3 ASW, which is isosmotic to the blood, thyrotrophs (TSH cells) corticotrophs (ACTH cells) and somatotrophs (STH cells) were not clearly affected. In DW, these cells and their nuclei were significantly enlarged. Their stimulation was also evident in 1/3 Ca-free ASW; values for cellular and nuclear areas were maximal in this environment and significantly higher than those of fish in FW and 1/3 ASW. These data suggest that in addition to the PAS-positive cells of the pars intermedia, highly stimulated in Ca-free environments, other cell types of the pars distalis may be involved in osmoregulation, and that the role of PRL cells is not primordial in the goldfish.
D000064:Acclimatization; D000324:Adrenocorticotropic Hormone / Q000032:analysis; D000818:Animals; D002118:Calcium; D002467:Cell Nucleus / Q000648:ultrastructure; D005640:Follicle Stimulating Hormone / Q000032:analysis; D006054:Goldfish; D013006:Growth Hormone / Q000032:analysis; D007986:Luteinizing Hormone / Q000032:analysis; D008854:Microscopy, Electron; D009994:Osmolar Concentration; D010902:Pituitary Gland / Q000166:cytology* / Q000502:physiology / Q000648:ultrastructure; D011388:Prolactin / Q000032:analysis; D013972:Thyrotropin / Q000032:analysis
D000324:Adrenocorticotropic Hormone; D011388:Prolactin; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D013972:Thyrotropin; D013006:Growth Hormone; D002118:Calcium