title: Photosensitization of mitochondrial adenosine-triphosphatase and adenylate kinase by hematoporphyrin derivative in vitro.
abstract: Mitochondrial ATPase and adenylate kinase activity of hepatoma cells were inhibited by hematoporphyrin derivative (HPD) followed by photoirradiation. Inhibition of ATPase activity was a dose- and time-related event. Malonaldehyde (MDA) content of mitochondrial membranes was markedly increased by HPD plus light. The content of mouse liver microsomal cytochrome P-450 was greatly increased after intraperitoneal injection of HPD for 4 days (5 mg/kg/day). The liver weight, and levels of liver microsomal G-6-phosphatase, MDA and triglyceride (TG) showed no difference in treated vs. control animals. The data presented here demonstrate that mitochondria may be a sensitive site of action of HPD photosensitization, and inactivation of ATPase and adenylate kinase may be an important contributing factor to tumor cell damage and death.
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism* / Q000528:radiation effects; D000263:Adenylate Kinase / Q000378:metabolism* / Q000528:radiation effects; D000818:Animals; D005260:Female; D005952:Glucose-6-Phosphatase / Q000378:metabolism / Q000528:radiation effects; D017324:Hematoporphyrin Derivative; D006414:Hematoporphyrin Photoradiation; D006415:Hematoporphyrins / Q000494:pharmacology*; D007700:Kinetics; D008114:Liver Neoplasms, Experimental / Q000188:drug therapy / Q000201:enzymology*; D051379:Mice; D010770:Phosphotransferases / Q000378:metabolism*; D011838:Radiation-Sensitizing Agents / Q000494:pharmacology*
chemical_list: D006415:Hematoporphyrins; D011838:Radiation-Sensitizing Agents; D017324:Hematoporphyrin Derivative; D010770:Phosphotransferases; D000263:Adenylate Kinase; D005952:Glucose-6-Phosphatase; D000251:Adenosine Triphosphatases