Preparation of highly purified human cytomegalovirus envelope antigen.
A human cytomegalovirus (HCMV) envelope preparation was highly purified by immunoaffinity column chromatography using an anti-cellular-IgG column. The purified envelope induced high titre antibodies to HCMV in guinea-pigs. Analysis of the guinea-pig immune sera by RIA and immunofluorescence (IF) showed that this envelope preparation, unlike its unpurified counterpart, did not induce antibody to cellular contaminants. Dot-blot assay revealed viral proteins in the flow-through fraction and cellular proteins in the bound fractions. Results of sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis of flow-through and bound fractions suggest that a number of proteins previously identified as virus-specific may, in fact, reflect cellular contamination of the envelope preparation, or crossreactivity of some virus-specific proteins with cellular proteins.
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D000956:Antigens, Viral / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D003587:Cytomegalovirus / Q000276:immunology*; D006168:Guinea Pigs; D007114:Immunization; D008970:Molecular Weight; D014759:Viral Envelope Proteins / Q000276:immunology* / Q000302:isolation & purification
D000914:Antibodies, Viral; D000956:Antigens, Viral; D014759:Viral Envelope Proteins