Synthesis of methyl 6-(ammonium 2-acetamido-2-deoxy-alpha-D-glucopyranosyl phosphate)-alpha-D-mannopyranoside and use of this compound for the determination of N-acetylglucosamine-1-phosphotransferase.
Methyl 6-(ammonium 2-acetamido-2-deoxy-alpha-D-glucopyranosyl phosphate)-alpha-D-mannopyranoside was synthesized and identified by 1H-n.m.r. and 13C-n.m.r. data, acid hydrolysis, and elemental analysis. It was utilized for the determination of UDP-N-acetylglucosamine-1-phosphotransferase in an assay procedure that employed methyl alpha-D-mannopyranoside as an acceptor. The assay product was identified and characterized by thin-layer chromatography with the title reference compound. The present technique does not require [32P]UDP-N-acetylglucosamine, but effectively uses commercially available UDP-[14C]GlcNAc.
D000117:Acetylglucosamine / Q000031:analogs & derivatives*; D000818:Animals; D002250:Carbon Radioisotopes; D002460:Cell Line; D002855:Chromatography, Thin Layer; D005347:Fibroblasts / Q000201:enzymology; D005958:Glucosephosphates / Q000138:chemical synthesis*; D006801:Humans; D007202:Indicators and Reagents; D008247:Lysosomes / Q000201:enzymology; D008360:Mannosephosphates; D008862:Microsomes, Liver / Q000201:enzymology; D009081:Mucolipidoses / Q000201:enzymology; D010770:Phosphotransferases / Q000378:metabolism*; D051381:Rats; D017855:Transferases (Other Substituted Phosphate Groups); D014537:Uridine Diphosphate N-Acetylglucosamine
D002250:Carbon Radioisotopes; D005958:Glucosephosphates; D007202:Indicators and Reagents; D008360:Mannosephosphates; D014537:Uridine Diphosphate N-Acetylglucosamine; C048076:methyl 6-(2-acetamido-2-deoxy-alpha-glucopyranosyl phosphate)-alpha-mannopyranoside; D010770:Phosphotransferases; D017855:Transferases (Other Substituted Phosphate Groups); C030012:UDP-N-acetylglucosamine-lysosomal-enzyme-N-acetylglucosaminephosphotransferase; D000117:Acetylglucosamine