Effect of dibutyryl cyclic AMP on morphologic features and biologic markers of a human salivary gland adenocarcinoma cell line in culture.
Dibutyryl cyclic AMP (dB-cAMP) has marked effects on the growth, morphologic features, and biologic markers of a human salivary gland adenocarcinoma cell line in culture. A cell line with ultrastructure and biologic markers specific to the intercalated duct cells of human salivary glands was cultivated in the presence of dB-cAMP. Major alterations, such as process formation and expression of myofilaments and oxytocin receptor in addition to myosin and S-100 protein, were observed in those cells with a phenotype similar to myoepithelial cells. Both the anchorage-independent and anchorage-dependent growths were markedly suppressed in the presence of dB-cAMP. After the removal of dB-cAMP from the culture, the treated cells returned rapidly to the phenotype of the untreated cells. These findings indicate that reversible differentiation into the myoepithelial cells of a human salivary gland adenocarcinoma cell line occurs in growth medium containing dB-cAMP.
D000230:Adenocarcinoma / Q000032:analysis / Q000473:pathology*; D001345:Autoradiography; D003994:Bucladesine / Q000494:pharmacology*; D002272:Carcinoembryonic Antigen / Q000032:analysis; D002453:Cell Cycle; D002460:Cell Line; D002999:Clone Cells; D003470:Culture Media; D006651:Histocytochemistry; D006801:Humans; D007124:Immunoenzyme Techniques; D008854:Microscopy, Electron; D009218:Myosins / Q000032:analysis; D011945:Receptors, Angiotensin / Q000032:analysis; D018045:Receptors, Oxytocin; D009418:S100 Proteins / Q000032:analysis; D012468:Salivary Gland Neoplasms / Q000032:analysis / Q000473:pathology*
D002272:Carcinoembryonic Antigen; D003470:Culture Media; D011945:Receptors, Angiotensin; D018045:Receptors, Oxytocin; D009418:S100 Proteins; D003994:Bucladesine; D009218:Myosins