Two different forms of cytochrome c oxidase can be purified from the slime mold Dictyostelium discoideum.
Cytochrome c oxidase was purified from mitochondria of Dictyostelium discoideum cells harvested at different phases of the vegetative stage. Comparison of the preparations by sodium dodecyl sulfate-polyacrylamide gel electrophoresis indicates that the smallest enzyme subunit present during exponential growth is replaced by a larger polypeptide component in the stationary phase. The two polypeptides are structurally and immunologically unrelated.
D000936:Antigen-Antibody Complex; D004023:Dictyostelium / Q000201:enzymology* / Q000254:growth & development; D003576:Electron Transport Complex IV / Q000302:isolation & purification* / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D007106:Immune Sera; D046911:Macromolecular Substances; D008970:Molecular Weight
D000936:Antigen-Antibody Complex; D007106:Immune Sera; D046911:Macromolecular Substances; D003576:Electron Transport Complex IV