Cloning of mini-mu bacteriophage in cosmids: in vivo packaging into phage lambda heads.
A technique has been developed that permits the packaging of mini-Mu-carrying cosmids into phage lambda heads. This procedure has several advantages over packaging into Mu helper capsids: the amounts of DNA to be packaged can be increased, the packaging efficiency is improved, and the stability of transducing lysates is high.
D010582:Bacteriophage lambda / Q000235:genetics*; D010583:Bacteriophage mu / Q000235:genetics*; D003001:Cloning, Molecular / Q000379:methods*; D003360:Cosmids; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors; D007763:Lac Operon; D014161:Transduction, Genetic
D004251:DNA Transposable Elements