Expression of chicken egg white lysozyme by Saccharomyces cerevisiae.
An efficient yeast promoter was isolated using a beta-galactosidase (beta Gal) promoter probe vector. This promoter was then used to express chicken egg white lysozyme in yeast using a complete intron-free lysozyme-coding sequence constructed by in vitro recombination between a cDNA clone lacking the 5' end and the corresponding 5' end from a nuclear DNA clone. The resulting lysozyme is efficiently exported into the growth medium suggesting that the chicken signal sequence is recognized by the yeast secretion process.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002645:Chickens; D004262:DNA Restriction Enzymes; D004251:DNA Transposable Elements; D004529:Egg White; D005260:Female; D005796:Genes; D005822:Genetic Vectors; D009113:Muramidase / Q000235:genetics*; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D001616:beta-Galactosidase / Q000235:genetics
D004251:DNA Transposable Elements; D004262:DNA Restriction Enzymes; D009113:Muramidase; D001616:beta-Galactosidase