Calcitonin and calcitonin gene-related peptide interact with the same receptor in cultured LLC-PK1 kidney cells.
Calcitonin and calcitonin gene-related peptide stimulate adenylate cyclase activity and plasminogen activator production in cultured renal tubular LLC-PK1 cells. Salmon [125I]calcitonin and human [125I]calcitonin gene-related peptide bound specifically to the cells. Salmon [125I]calcitonin binding was reduced at lower concentrations of non-radioactive salmon calcitonin than of human calcitonin gene-related peptide. For the stimulation of adenylate cyclase activity and plasminogen activator production, the potency of salmon calcitonin was higher than that of human calcitonin and calcitonin gene-related peptide. In a subclone of LLC-PK cells lacking salmon calcitonin binding sites, no specific binding of [125I]CGRP occurred, and adenylate cyclase activity and plasminogen activator production was not increased by the peptides. Thus, in LLC-PK1 cells the stimulation of adenylate cyclase activity and plasminogen activator production by calcitonin gene-related peptide is probably mediated by the calcitonin receptor.
D000262:Adenylyl Cyclases / Q000378:metabolism; D000818:Animals; D002116:Calcitonin / Q000378:metabolism* / Q000494:pharmacology; D015740:Calcitonin Gene-Related Peptide; D002460:Cell Line; D006801:Humans; D007684:Kidney Tubules / Q000187:drug effects / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000378:metabolism* / Q000494:pharmacology; D010960:Plasminogen Activators / Q000096:biosynthesis; D018003:Receptors, Calcitonin; D011956:Receptors, Cell Surface / Q000187:drug effects / Q000502:physiology*; D013552:Swine; D014667:Vasopressins / Q000494:pharmacology
D009419:Nerve Tissue Proteins; D018003:Receptors, Calcitonin; D011956:Receptors, Cell Surface; D014667:Vasopressins; D002116:Calcitonin; D010960:Plasminogen Activators; D000262:Adenylyl Cyclases; D015740:Calcitonin Gene-Related Peptide