Transient replication of bovine papilloma virus type 1 plasmids: cis and trans requirements.
A transient assay has been used to study bovine papilloma virus type 1 (BPV-1) replication. We show that BPV-1 early replication occurs faster than cellular DNA synthesis. Initial replication events are dependent on a gene product(s), encoded by the BPV-1 E1 open reading frame. Mutational analysis of the viral upstream regulatory region shows the requirement of two domains in cis for replication. Domain one, located outside of the viral 69% transforming fragment, is an enhancer-like activity and can be replaced by other known viral enhancers. Domain two lies within sequences previously defined as plasmid maintenance sequence 1. The apparent requirement for a proximal enhancer function for replication may explain why certain BPV-1 constructions, when linked to bacterial plasmid sequences, can be maintained extrachromosomally while others cannot.
D052688:Bovine papillomavirus 1 / Q000235:genetics / Q000254:growth & development*; D002874:Chromosome Mapping; D004278:DNA, Superhelical / Q000096:biosynthesis; D004279:DNA, Viral / Q000096:biosynthesis; D004742:Enhancer Elements, Genetic; D005786:Gene Expression Regulation; D027383:Papillomaviridae / Q000254:growth & development*; D010957:Plasmids; D012093:Replicon; D013997:Time Factors; D014158:Transcription, Genetic; D014779:Virus Replication
D004278:DNA, Superhelical; D004279:DNA, Viral