Isolation of cytophatic strains of rotavirus from pigs.
Twenty-two rotavirus isolates were recovered from piglets suffering from diarrhea. The isolates readily propagated in MA-104 cell cultures where they induced typical cytopathic effects (CPE) and possessed the physicochemicals properties of the members of rotavirus genus, Family Reoviridae. The electron microscopy study, conducted in MA-104 infected cells, revealed virus particles which possessed the peculiar morphology of rotavirus. The isolates were neutralized by a reference porcine rotavirus antiserum: all were devoid of hemagglutinating activity for red cells of swine, cattle, sheep, rabbit, chicken and guinea pig.
D000818:Animals; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D003967:Diarrhea / Q000382:microbiology / Q000662:veterinary*; D005243:Feces / Q000382:microbiology; D008854:Microscopy, Electron; D012401:Rotavirus / Q000302:isolation & purification* / Q000502:physiology / Q000648:ultrastructure; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*