Antibodies against platelet-derived growth factor inhibit acute transformation by simian sarcoma virus.
A clue to the molecular mechanism of neoplastic transformation was provided by the finding of a near identity in amino-acid sequence between the platelet-derived growth factor (PDGF) B-chain and a region in the transforming protein, p28sis, of simian sarcoma virus (SSV), an agent that causes sarcomas and gliomas in experimental animals. This finding infers a direct link between the molecular biology of normal mitogenesis and oncogenesis since it suggests that the transforming activity of SSV is caused by a growth factor. Although PDGF agonist activity has been isolated from conditioned medium of SSV-transformed cells, it is not clear whether infection of responsive cells by SSV leads solely to autocrine stimulation of growth by a secreted PDGF-like factor or whether other, possibly intracellular, activities of p28sis or its processed products contribute to the transformation. To distinguish between these possibilities, we have studied the effect of anti-PDGF antibodies on acute SSV-transformation, and report here that these antibodies inhibit both proliferation and SSV-induced morphological changes in human diploid fibroblasts.
D000906:Antibodies; D002455:Cell Division / Q000187:drug effects; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D002472:Cell Transformation, Viral / Q000187:drug effects*; D003429:Cross Reactions; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D010455:Peptides / Q000032:analysis / Q000276:immunology; D010982:Platelet-Derived Growth Factor / Q000032:analysis / Q000276:immunology*; D012190:Retroviridae / Q000472:pathogenicity*; D012842:Sarcoma Virus, Woolly Monkey / Q000235:genetics / Q000472:pathogenicity*; D015291:Transforming Growth Factors
D000906:Antibodies; D010455:Peptides; D010982:Platelet-Derived Growth Factor; D015291:Transforming Growth Factors