Cross-reactivity of a monoclonal antibody to the catalytic subunit of chicken gizzard desmin-specific calpain.
The desmin-specific calpain I from chicken gizzard smooth muscle is a dimer of 83 and 35 kDalton subunits. A monoclonal antibody to the large subunit did not cross-react with chicken gizzard and hamster skeletal muscle calpain II, but it did recognize hamster skeletal muscle desmin-specific calpain I and the denatured calpain II from chicken gizzard smooth muscle. These results indicate that different desmin-specific calpains have similar large subunits which differ significantly from the large subunit of calpain II in the same tissue.
D000818:Animals; D000911:Antibodies, Monoclonal; D002154:Calpain / Q000276:immunology* / Q000378:metabolism; D002645:Chickens; D006224:Cricetinae; D003429:Cross Reactions; D003893:Desmin / Q000378:metabolism*; D005895:Gizzard, Avian / Q000201:enzymology; D046911:Macromolecular Substances; D008970:Molecular Weight; D009130:Muscle, Smooth / Q000201:enzymology*; D009132:Muscles / Q000201:enzymology; D013379:Substrate Specificity
D000911:Antibodies, Monoclonal; D003893:Desmin; D046911:Macromolecular Substances; D002154:Calpain