Influence of receptor formation and receptor movement inhibitors on hormonal imprinting in cell culture.
Hormonal imprinting takes place at the first interaction of the cell with the adequate hormone, and exerts a lasting influence on cellular binding capacity and functional response over many subsequent cell generations. Hormonal imprinting can also be induced in cell lines. In a Chinese hamster ovary (CHO K1) cell line, inhibitor of endocytosis and cellular protein synthesis inhibited hormone binding in themselves, and in cultures preexposed to TSH they inhibited imprinting by TSH in a dose-dependent manner. The protein synthesis inhibitor cycloheximide and the microfilament de-organizing agent cytochalasin-B inhibited imprinting by TSH to a greater degree than all other inhibitors tested, indicating that apart from cellular binding capacity, unimpaired cellular protein synthesis and microfilament activity are essential prerequisites of hormonal imprinting.
D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D003513:Cycloheximide / Q000494:pharmacology; D003571:Cytochalasin B / Q000378:metabolism; D003599:Cytoskeleton / Q000378:metabolism; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D005260:Female; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D005640:Follicle Stimulating Hormone / Q000378:metabolism; D006728:Hormones / Q000502:physiology*; D010053:Ovary / Q000166:cytology*; D011956:Receptors, Cell Surface / Q000502:physiology*; D013861:Thiocyanates; D013972:Thyrotropin / Q000378:metabolism
D005452:Fluoresceins; D006728:Hormones; D011956:Receptors, Cell Surface; D013861:Thiocyanates; D003571:Cytochalasin B; D005640:Follicle Stimulating Hormone; D013972:Thyrotropin; D003513:Cycloheximide; D016650:Fluorescein-5-isothiocyanate; D004121:Dimethyl Sulfoxide