Multiple bluetongue virus-cloned genetic probes: application to diagnostics and bluetongue virus genetic relationships.
A shotgun-cloning method incorporating all 10 bluetongue virus genome segments can simultaneously produce complete and partial copies of any of the genome segments. We report here 4 different cloned probes derived from 3 genome segments and individually defined by different hybridization recognition capabilities. One probe hybridized strongly with all 5 United States prototype strains of the 5 different bluetongue virus (BTV) serotypes existing in the United States and, as such, is a strong candidate for a broad BTV diagnostic probe in the United States. Another probe derived from genome segment 2 of BTV-17 hybridized only with the BTV-17 prototypic serotype, thereby demonstrating serospecific hybridization diagnostic potential. The implications for diagnostic and genetic relationship studies on BTV, using various genetic probes, are discussed.
D000818:Animals; D001820:Bluetongue virus / Q000235:genetics*; D002460:Cell Line; D003001:Cloning, Molecular; D006224:Cricetinae; D004247:DNA / Q000378:metabolism; D005814:Genes, Viral; D007668:Kidney; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D012330:RNA, Double-Stranded / Q000235:genetics / Q000302:isolation & purification; D012087:Reoviridae / Q000235:genetics*; D012703:Serotyping
D012330:RNA, Double-Stranded; D004247:DNA