title: Effect of 2,4-dinitrofluorobenzene on the enzymatic properties of the b-c1 complex isolated from beef heart mitochondria.
abstract: A study is presented on the effect of 2,4-dinitrofluorobenzene (DFNB) on the enzymatic properties of mitochondrial b-c1 complex. The chemical modification by DNFB strongly inhibits the reductase activity of the complex, this being accompanied by labelling by [3H]DNFB of core protein I, the apoprotein of b cytochromes and the 12 kDa subunit. Chemical modification by DNFB appears to alter, in particular, the domain of heme b-562.
mesh_terms: D000818:Animals; D002417:Cattle; D003573:Cytochrome b Group / Q000037:antagonists & inhibitors / Q000378:metabolism; D003575:Cytochromes c1 / Q000037:antagonists & inhibitors / Q000378:metabolism; D004139:Dinitrofluorobenzene / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004579:Electron Transport / Q000187:drug effects; D066298:In Vitro Techniques; D007700:Kinetics; D008929:Mitochondria, Heart / Q000187:drug effects* / Q000201:enzymology; D009578:Nitrobenzenes / Q000494:pharmacology*
chemical_list: D003573:Cytochrome b Group; D009578:Nitrobenzenes; D003575:Cytochromes c1; D004139:Dinitrofluorobenzene