Density-gradient sedimentation of feline bone marrow cells with polyvinyl-pyrrolidone-coated silica gel.
Feline bone marrow cells were separated based on their density distribution in a continuous polyvinyl-pyrrolidone-coated silica gel density gradient. Morphologic identification of cytocentrifuged preparations at specific densities revealed a progressive increase in density with maturation of the cells within the granulocytic and erythroid series. Segmented eosinophils peaked at 1.084 g/ml, monocytes at 1.07 g/ml, and lymphocytes spanned the density of 1.068 to 1.084 g/ml. Bone marrow samples from 6 healthy specific-pathogen-free cats were separated by the continuous polyvinyl-pyrrolidone-coated silica gel gradient and were studied for progenitor colony formation in methylcellulose. Erythroid colony formation was greatest at a density of 1.084 g/ml and also appeared in cells from 4 cats at a lighter density of 1.016 to 1.05 g/ml. Colony formation in the granulocytic series revealed progenitors throughout the gradient with enrichment at 1.055 g/ml.
D000818:Animals; D001854:Bone Marrow Cells; D002415:Cats; D002469:Cell Separation / Q000379:methods; D002499:Centrifugation, Density Gradient / Q000379:methods; D006412:Hematopoietic Stem Cells / Q000166:cytology; D011205:Povidone; D012822:Silicon Dioxide; D013047:Specific Pathogen-Free Organisms
D012822:Silicon Dioxide; D011205:Povidone