Characterization of biological properties of synthetic and biological leukotriene B3.
Leukotriene B3 was chemically synthesized and its ability to aggregate rat polymorphonuclear leukocytes (PMN) and to enhance chemokinesis of human leukocytes demonstrated. In both these assays the potency of synthetic leukotriene B3 was marginally less than that of leukotriene B4. Rat PMN incubated with leukotriene A3 were very inefficient in the enzymatic conversion of this epoxide to leukotriene B3. However, the leukotriene B3 produced was able to aggregate rat PMN. These results suggest that unlike leukotriene B5, the proinflammatory properties of leukotriene B3 are similar to those of leukotriene B4. However, since the enzymatic conversion of leukotriene A3 to leukotriene B3 is extremely poor it seems unlikely that leukotriene B3 itself has any major role in vivo.
D000818:Animals; D001095:Arachidonic Acids / Q000378:metabolism; D002449:Cell Aggregation / Q000187:drug effects; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D017572:Leukotriene A4; D007975:Leukotriene B4 / Q000096:biosynthesis / Q000138:chemical synthesis / Q000494:pharmacology* / Q000031:analogs & derivatives; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism; D051381:Rats
D001095:Arachidonic Acids; D017572:Leukotriene A4; D007975:Leukotriene B4; C045722:leukotriene B3