Isolation and sequencing of a cDNA clone homologous to the v-sis oncogene from human endothelial cells.
A clone containing the 3' end of the mRNA for the human c-sis gene (homologous to the B chain of platelet-derived growth factor) was isolated from a cDNA library derived from human umbilical vein endothelial cells and then sequenced. The analysis of possible translation products in all three reading frames indicated that the A chain of platelet-derived growth factor was not coded for within the 3' end of the c-sis mRNA. The 3' end of the mRNA for c-sis is contained in or adjacent to exon 6.
D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification*; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004727:Endothelium / Q000032:analysis; D006801:Humans; D009857:Oncogenes; D010982:Platelet-Derived Growth Factor / Q000235:genetics; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism
D010982:Platelet-Derived Growth Factor; D012333:RNA, Messenger; D004247:DNA; D004262:DNA Restriction Enzymes