title: Localization of the antigotensin-converting enzyme activity in testis and epididymis.
abstract: Angiotensin-converting enzyme (ACE) has been studied in different reproductive organs of the male rat, in somatic cell lines clonally derived from both rat and mouse testes, and in isolated spermatogenic cells of the mouse. Among the various reproductive organs only testis and epididymis show high levels of enzyme activity. The testicular activity is found mainly in the isolated germinal cells and residual bodies, whereas somatic cell lines contain negligible levels of activity even after addition of hormones and growth factors. Testicular homogenates, spermatogenic cells, epididymal spermatozoa, and spermatozoan cytoplasmic droplets, when fractionated by anion exchange chromatography, contain one major and one minor activity peak, whereas epididymal homogenates and epididymal secretions reveal an additional major activity peak together with the minor peak. All forms of ACE have a similar response to different modifiers, and are equally sensitive to the specific inhibitor N-[(S)-1-(ethoxycarbonyl)-3-phenylpropyl]-L-alanyl-L-proline (Enalapril). The testicular enzyme could provide a useful marker for spermatogenic maturation and/or cytoplasmic processes both in testis and epididymis. The separate epididymal peak is a secretory enzyme that may be responsible for the processing of spermatozoan plasma membrane constituents during epididymal transit, or may have a role in attacking some biologically active compounds.
mesh_terms: D000818:Animals; D002412:Cations; D002460:Cell Line; D002478:Cells, Cultured; D002614:Chelating Agents / Q000494:pharmacology; D002999:Clone Cells; D004822:Epididymis / Q000201:enzymology*; D007700:Kinetics; D007985:Leydig Cells / Q000201:enzymology; D008297:Male; D051379:Mice; D007703:Peptidyl-Dipeptidase A / Q000378:metabolism*; D051381:Rats; D012708:Sertoli Cells / Q000201:enzymology; D013737:Testis / Q000166:cytology / Q000201:enzymology*
chemical_list: D002412:Cations; D002614:Chelating Agents; D007703:Peptidyl-Dipeptidase A