Unique pyrimidine 2D-COSY aromatic cross-peaks as monitors of pyrimidine environments and mobility in oligo- and polynucleotides.
Only cytosine contains two adjacent aromatic protons that give rise to cross-peaks in the aromatic region of 2D-COSY spectra of oligodeoxynucleotides. In two GC-containing sequences several such cross-peaks were resolved. The intensity of these cross-peaks is a sensitive monitor of local mobility, and upon the addition of the intercalating drug daunomycin selective intensity losses were observed, indicating binding to specific GC base pairs. We have also monitored the 2D-COSY cross-peaks from mobile pyrimidine bases in tRNA (Phe) as a function of temperature.
D001482:Base Composition; D001665:Binding Sites; D003562:Cytidine / Q000032:analysis; D003630:Daunorubicin / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D009841:Oligonucleotides / Q000032:analysis*; D011522:Protons; D011742:Pyrimidine Nucleotides / Q000032:analysis*; D011743:Pyrimidines / Q000032:analysis*; D012343:RNA, Transfer / Q000032:analysis*; D013696:Temperature
D009841:Oligonucleotides; D011522:Protons; D011742:Pyrimidine Nucleotides; D011743:Pyrimidines; D003562:Cytidine; D012343:RNA, Transfer; D003630:Daunorubicin