Use of DNA probes from the 5' flanking region of the HLA-B gene to examine polymorphism at the HLA-B locus.
Two DNA probes isolated from the region 5' to the HLA-B gene are described. One of these, pCH6, detects a TaqI polymorphism which is correlated with serological alleles of HLA-B. Both probes are used to show a high level of DNA sequence homology between the 5' flanking region of HLA-B and HLA-C.
D002874:Chromosome Mapping; D003101:Collodion; D004247:DNA / Q000302:isolation & purification; D004262:DNA Restriction Enzymes; D004591:Electrophoresis, Polyacrylamide Gel; D005819:Genetic Markers; D006680:HLA Antigens / Q000032:analysis* / Q000235:genetics; D015235:HLA-B Antigens; D015796:HLA-B27 Antigen; D015236:HLA-C Antigens; D006801:Humans; D006824:Hybridization, Genetic; D010209:Paper; D011110:Polymorphism, Genetic; D013166:Spondylitis / Q000097:blood
D005819:Genetic Markers; D006680:HLA Antigens; D015235:HLA-B Antigens; D015796:HLA-B27 Antigen; D015236:HLA-C Antigens; D003101:Collodion; D004247:DNA; D004262:DNA Restriction Enzymes