Transposition of the gram-positive transposon Tn917 in Escherichia coli.
The streptococcal transposon Tn917 was demonstrated to transpose in Escherichia coli from the Bacillus subtilis-E. coli shuttle plasmid pHK1207 into an F' plasmid derivative. Subsequently, a second round of transposition from the F' plasmid into pACYC184 could be readily demonstrated. These results represent the initial demonstration of the transposition of a gram-positive transposon in a gram-negative bacterium at a relatively high frequency.
D001412:Bacillus subtilis / Q000235:genetics; D004251:DNA Transposable Elements; D004352:Drug Resistance, Microbial; D004917:Erythromycin / Q000494:pharmacology; D004926:Escherichia coli / Q000235:genetics*; D005144:F Factor; D005822:Genetic Vectors; D010957:Plasmids; D011995:Recombination, Genetic
D004251:DNA Transposable Elements; D004917:Erythromycin