The response of an established line of rat liver cells to thyroid hormone.
The response of an established line of non-transformed adult rat liver epithelial cells (ARL 15) to thyroid hormone (T3) (3,5,3'-triiodothyronine) was characterized. Exposure of confluent monolayers to 1.10(-8) M T3 for 3 days increased O2 consumption (QO2) between 14-58%, ouabain-sensitive Rb+ uptake 26%, (Na+ + K+)-ATPase activity 32%, alpha-glycerophosphate dehydrogenase activity 103% and cytochrome oxidase activity 208%. The ARL 15 cells, maintained in continuous culture, therefore, exhibit the hallmarks of an authentic physiological response to thyroid hormone.
D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D002460:Cell Line; D003576:Electron Transport Complex IV / Q000378:metabolism; D005993:Glycerolphosphate Dehydrogenase / Q000378:metabolism; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D010101:Oxygen Consumption / Q000187:drug effects; D051381:Rats; D012413:Rubidium / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D014284:Triiodothyronine / Q000494:pharmacology*
D014284:Triiodothyronine; D005993:Glycerolphosphate Dehydrogenase; D003576:Electron Transport Complex IV; D000254:Sodium-Potassium-Exchanging ATPase; D012413:Rubidium