title: Translocation of cAMP-dependent protein kinase to the nucleus during development of Dictyostelium discoideum.
abstract: The distribution of the catalytic and regulatory subunits of the cAMP-dependent protein kinase between cytoplasm and nucleus was determined during the development of Dictyostelium discoideum. In vegetative amoebae approximately 2% of the subunits were in the nucleus. During development there was an approximately 5-fold increase in total soluble cAMP-dependent protein kinase and a 15- to 30-fold increase of enzyme in the nuclear fraction. There was a reverse translocation from nucleus to cytoplasm, when Tipped Aggregates were disrupted and the resultant amoebae incubated in single-cell suspension. The addition of cAMP to these single-cell suspensions brought about the reentry of the subunits into the nucleus. The findings are discussed in relation to the potential role of the cAMP-dependent protein kinase in the regulation of mRNA and protein synthesis.
mesh_terms: D001692:Biological Transport; D002467:Cell Nucleus / Q000201:enzymology; D000242:Cyclic AMP / Q000494:pharmacology*; D003593:Cytoplasm / Q000201:enzymology; D004023:Dictyostelium / Q000201:enzymology / Q000254:growth & development*; D004591:Electrophoresis, Polyacrylamide Gel; D007700:Kinetics; D011494:Protein Kinases / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D000242:Cyclic AMP; D011494:Protein Kinases