Sequences of papillomavirus DNA in equine sarcoids.
DNA was extracted from 14 equine sarcoids, electrophoresed and hybridised with a radioactively labelled probe of bovine papillomavirus type I (BPV 1) DNA under conditions of low stringency. Twelve sarcoids contained sequences of DNA that hybridised with the probe and that comigrated with BPV 2 DNA. The viral DNAs in four of these sarcoids differed from BPV 1 and BPV 2 DNA on restriction endonuclease analysis. One of four cell lines derived from sarcoids also contained BPV 1 related DNA. The results confirm the frequent presence in equine sarcoids of unintegrated papillomaviral DNA and suggest a role for papillomavirus infection in this disease.
D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D004279:DNA, Viral / Q000032:analysis*; D004586:Electrophoresis; D006734:Horse Diseases / Q000382:microbiology*; D006736:Horses; D009693:Nucleic Acid Hybridization; D027383:Papillomaviridae / Q000032:analysis*; D012507:Sarcoidosis / Q000382:microbiology / Q000662:veterinary*
D004279:DNA, Viral