Immunoperoxidase plaque staining for the detection of pseudorabies virus.
A quantitative indirect immunoperoxidase plaque staining method was developed for the detection of pseudorabies virus infection in a pig kidney cell line (PK-15). The method is rapid and specific and foci of infection, represented by stained plaques, are easily counted by the unaided eye. Possible modification of this technique in a plaque reduction assay for the detection of antipseudorabies virus antibody is also discussed.
D000818:Animals; D011558:Herpesvirus 1, Suid / Q000302:isolation & purification*; D007124:Immunoenzyme Techniques; D013552:Swine; D010948:Viral Plaque Assay / Q000379:methods*