A simple 'free 3H-ligand' assay for rapid screening of hybridoma monoclonal antibodies against neurotransmitter analogs and anti-idiotype antibodies.
Effective, rapid screening of hybridoma supernatants for monoclonal antibodies against the dopaminergic antagonists pimozide and haloperidol, and the serotonergic antagonist ketanserin was performed using a 'free 3H-ligand' assay. Anti-mouse Ig-coated microtiter plates were incubated with hybridoma supernatants prior to incubation with excess 3H-ligand. After removal of free 3H-ligand, bound 3H-ligand was eluted with acid for liquid scintillation counting. With minor modification, the assay can be used to screen hybridomas for anti-anti-ligand (anti-idiotypic) antibodies.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D006220:Haloperidol / Q000276:immunology*; D006825:Hybridomas / Q000276:immunology; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D007650:Ketanserin; D051379:Mice / Q000276:immunology; D010868:Pimozide / Q000276:immunology*; D010880:Piperidines / Q000276:immunology*; D017981:Receptors, Neurotransmitter / Q000276:immunology
D000911:Antibodies, Monoclonal; D007130:Immunoglobulin Idiotypes; D010880:Piperidines; D017981:Receptors, Neurotransmitter; D010868:Pimozide; D007650:Ketanserin; D006220:Haloperidol