Correlation of cell-surface phenotype with the establishment of interleukin 3-dependent cell lines from wild-mouse murine leukemia virus-induced neoplasms.
The wild mouse ecotropic virus, Cas-Br-M murine leukemia virus, induces myeloid and erythroid leukemias as well as T-cell and B-cell lymphomas in NFS mice. The ability to establish long-term cell lines from these tumors in the presence or absence of the T-cell-derived lymphokine interleukin 3 (IL-3) was examined. IL-3-dependent cell lines were readily obtained from the majority of the myeloid or erythroid leukemias and immunoblastic lymphomas. In the absence of IL-3, only one long-term factor-independent cell line was obtained from a myelogenous leukemia. The majority of the thymic T-cell lymphomas or B-lineage lymphomas could not be cultured in the presence or absence of IL-3. The results suggest that transformation of hematopoietic lineages does not necessarily obviate the requirement for normal growth factors. The acquisition of independence from growth factors may require additional transforming events.
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D002472:Cell Transformation, Viral; D007377:Interleukin-3; D009052:Leukemia Virus, Murine; D004915:Leukemia, Erythroblastic, Acute / Q000209:etiology / Q000473:pathology; D007942:Leukemia, Experimental / Q000209:etiology / Q000473:pathology*; D007951:Leukemia, Myeloid / Q000209:etiology / Q000473:pathology; D008222:Lymphokines / Q000494:pharmacology / Q000502:physiology*; D008223:Lymphoma / Q000209:etiology / Q000473:pathology; D008228:Lymphoma, Non-Hodgkin / Q000209:etiology / Q000473:pathology; D008232:Lymphoproliferative Disorders / Q000209:etiology / Q000473:pathology*; D051379:Mice; D010641:Phenotype; D013953:Thymus Neoplasms / Q000209:etiology / Q000473:pathology
D007377:Interleukin-3; D008222:Lymphokines