Scanning molecular sieve chromatography of interacting protein systems: nonlinear least-squares analysis of small zone experiments.
A simple routine for nonlinear least-squares analysis is applied to small zone scanning data, where calibration of the gel column requires the use of fully characterized markers of known molecular size. Application of nonlinear least-squares analysis eliminates the difficulty encountered because of scarcity of calibrating markers for gels whose porosities span certain size ranges by providing a sensitive measure of changes in molecular size of the interacting protein system, in order to determine the interaction parameters, size heterogeneity, and the centroid position for time difference chromatographic experiments.
D001055:Apolipoproteins B / Q000302:isolation & purification; D001699:Biometry; D002850:Chromatography, Gel / Q000379:methods*; D003574:Cytochrome c Group / Q000302:isolation & purification; D004655:Emulsions; D006801:Humans; D008077:Lipoproteins, LDL / Q000302:isolation & purification; D011506:Proteins / Q000302:isolation & purification*; D012984:Software; D013053:Spectrophotometry
D001055:Apolipoproteins B; D003574:Cytochrome c Group; D004655:Emulsions; D008077:Lipoproteins, LDL; D011506:Proteins