Protected 2'-deoxyaristeromycin on polymer support: a substitute for the acid labile 2'-deoxyadenosine moiety at the 3'-terminus of oligodeoxynucleotides.
A modified nucleoside antibiotic on polymer support was employed in the solid-phase synthesis of two oligodeoxynucleotides to substitute acid labile 2'-deoxyadenosine(A) at the 3'-terminus of oligomers with acid resistant 2'-deoxyaristeromycin(Ar). The duplex of the decamer having sticky ends was inserted successfully into the Eco R1 site of pBR322. E. coli DH 1 was transformed with the Ar-containing plasmid. Inspection of the cloned plasmid pBR2552 by restriction endonucleases revealed that no deletion and insertion occurred near the inserted sequence. This result indicates that Ar can behave as A in DNA replication of the host cells. Therefore, Ar can be used as a substitute for 3'-terminal A in the solid-phase oligonucleotide synthesis.
D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D003839:Deoxyadenosines / Q000031:analogs & derivatives*; D004926:Escherichia coli / Q000235:genetics; D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis*; D010957:Plasmids
D003839:Deoxyadenosines; D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides; C047923:2'-deoxyaristeromycin; D004262:DNA Restriction Enzymes