The role of haemagglutinin-neuraminidase glycoprotein cell surface expression in the survival of Sendai virus-infected BHK-21 cells.
A temperature-sensitive mutant of Sendai virus with a lesion in the haemagglutinin-neuraminidase protein (HN) (ts 271) was used to study the effect of HN cell surface expression on the fate of infected BHK-21 cells. The total amount of HN was reduced in ts 271 virus-infected cells at the non-permissive temperature (38 degrees C) presumably due to degradation of the protein. At this temperature, neither HN nor a modified form of HN were found expressed at the surface of the infected cells. BHK-21 cells infected with ts 271 were nevertheless killed by the infection at 38 degrees C as well as at 30 degrees C. These results ruled out the hypothesis that the lack of HN cell surface expression could be the unique requirement allowing BHK cell survival.
D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000032:analysis*; D002470:Cell Survival; D006224:Cricetinae; D003588:Cytopathogenic Effect, Viral; D015707:HN Protein; D006389:Hemagglutinins, Viral / Q000032:analysis / Q000502:physiology; D007668:Kidney; D009154:Mutation; D009439:Neuraminidase / Q000032:analysis / Q000502:physiology; D010222:Parainfluenza Virus 1, Human / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D013696:Temperature; D014764:Viral Proteins / Q000032:analysis / Q000096:biosynthesis / Q000502:physiology*
D015707:HN Protein; D006389:Hemagglutinins, Viral; D014764:Viral Proteins; D009439:Neuraminidase