title: Mechanisms of in situ nick translation of chromosomes using restriction endonucleases.
abstract: In situ nick translation of mammalian chromosomes by restriction endonuclease treatment to nick the chromosomal DNA, and 'translation' in the presence of DNA polymerase I and biotinylated dUTP, results in a distinct banding pattern. Further experiments have elucidated the mechanisms producing these bands. The hypothesis is presented that differences in the local conformation of the DNA-protein complex, rather than the DNA sequence itself, lead to the nick translation bands. The different DNase I sensitivity along the chromosomes suggests that the bands, which were clearly evident, reflect morphological units closely related to biological functions.
mesh_terms: D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D002875:Chromosomes / Q000502:physiology*; D004262:DNA Restriction Enzymes; D003850:Deoxyribonuclease I; D006801:Humans; D007621:Karyotyping; D008214:Lymphocytes / Q000166:cytology; D014176:Protein Biosynthesis
chemical_list: D004262:DNA Restriction Enzymes; D003850:Deoxyribonuclease I