title: Chromate, molybdate, tungstate and vanadate behave as substrates of yeast diadenosine 5',5'''-p1,p4-tetraphosphate alpha, beta-phosphorylase.
abstract: Diadenosine 5',5'''-p1,p4-tetraphosphate (Ap4A) alpha, beta-phosphorylase from yeast Saccharomyces cerevisiae catalyzes two reactions: Ap4A cleavage and nucleoside diphosphate--phosphate (NDP-Pi) exchange. In both reactions phosphate can be substituted by arsenate, chromate, molybdate, tungstate or vanadate. In the presence of each anion, nucleoside 5'-monophosphate (NMP) always accumulates as a product of the reaction. This indicates that an unstable NMP anion is formed as an intermediate.
mesh_terms: D000838:Anions; D002840:Chromates / Q000378:metabolism*; D008982:Molybdenum / Q000378:metabolism*; D009711:Nucleotides / Q000378:metabolism; D009713:Nucleotidyltransferases / Q000378:metabolism*; D010710:Phosphates / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D013379:Substrate Specificity; D014414:Tungsten / Q000378:metabolism*; D017973:Tungsten Compounds; D014638:Vanadates; D014639:Vanadium / Q000378:metabolism*
chemical_list: D000838:Anions; D002840:Chromates; D009711:Nucleotides; D010710:Phosphates; D017973:Tungsten Compounds; D014639:Vanadium; C044659:molybdate; D014638:Vanadates; D008982:Molybdenum; D009713:Nucleotidyltransferases; C044740:diadenosine 5,5'''-P(1),P(4)-tetraphosphate alpha,beta-phosphorylase; C034944:chromic acid; C045951:tungstate; D014414:Tungsten