Stable expression of two variable surface glycoproteins by cloned Trypanosoma equiperdum.
African trypanosomes are thought to evade the host immune system by periodically changing their variable surface glycoprotein (VSG). VSG genes are activated by a complex process involving the duplicative transposition of silent basic copy genes to one of several expression sites. These expression-linked copies (ELCs) of the VSG genes are also subject to regulation within expression sites by as yet unknown mechanisms. It is generally assumed that trypanosomes can express only one VSG gene at a time. Nevertheless, the finding that they contain multiple VSG gene expression sites suggests that multiple expression is possible. We show here that Trypanosoma equiperdum can stably express two VSG genes in a simple axenic culture system and that both antigens are present on the cell surface. The two antigens do not co-cap or form heterodimers. Their corresponding genes show no cross-hybridization and are situated in different telomere-linked expression sites. Northern blot analysis reveals that both genes are active in the double expressors.
D000818:Animals; D002850:Chromatography, Gel; D004262:DNA Restriction Enzymes; D005455:Fluorescent Antibody Technique; D005796:Genes; D006023:Glycoproteins / Q000235:genetics* / Q000302:isolation & purification; D008970:Molecular Weight; D014345:Trypanosoma / Q000235:genetics*; D014643:Variant Surface Glycoproteins, Trypanosoma
D006023:Glycoproteins; D014643:Variant Surface Glycoproteins, Trypanosoma; D004262:DNA Restriction Enzymes