Axial ligands of chloroplast cytochrome b-559: identification and requirement for a heme-cross-linked polypeptide structure.
Optical, resonance Raman, and electron paramagnetic resonance spectroscopies have been used to characterize the ligands and spin state of the chloroplast cytochrome b-559. The protein was isolated from both maize and spinach in a low-potential form. The spectroscopic data indicate that the heme iron in both ferric and ferrous cytochrome b-559 is in its low-spin state and ligated in its fifth and sixth coordination positions by histidine nitrogens. Electron paramagnetic resonance data for the purified spinach cytochrome are in good agreement with those determined by Bergström and Vänngård [Bergström, J., & Vänngård, T. (1982) Biochim. Biophys. Acta 682, 452-456] for a low-potential membrane-bound form of cytochrome b-559. The g values of high-potential cytochrome b-559 are shifted from those of its low-potential forms; this shift is interpreted as arising from a deviation of the planes of the two axial histidine imidazole rings from a parallel orientation. The model is consistent with the physical data and may also account for the facility with which cytochrome b-559 can be converted between low- and high-potential forms. Recent biochemical and molecular biological data [Widger, W. R., Cramer, W. A., Hermodson, M., Meyer, D., & Gullifor, M. (1984) J. Biol. Chem. 259, 3870-3876; Herrmann, R. G., Alt, J., Schiller, D., Cramer, W. A., & Widger, W. R. (1984) FEBS Lett. 179, 239-244] have shown that two polypeptides, one with 83 residues and a second with 39 residues, most likely constitute the protein of the cytochrome.(ABSTRACT TRUNCATED AT 250 WORDS)
D001665:Binding Sites; D002736:Chloroplasts / Q000378:metabolism*; D003573:Cytochrome b Group / Q000378:metabolism*; D004578:Electron Spin Resonance Spectroscopy; D006418:Heme / Q000378:metabolism; D046911:Macromolecular Substances; D045332:Photosystem II Protein Complex; D010944:Plants / Q000378:metabolism*; D011485:Protein Binding; D013053:Spectrophotometry; D013059:Spectrum Analysis, Raman
D003573:Cytochrome b Group; D046911:Macromolecular Substances; D045332:Photosystem II Protein Complex; D006418:Heme; C019313:cytochrome b559