Assignment of the gene for the beta subunit of thyroid-stimulating hormone to the short arm of human chromosome 1.
The chromosomal locations of the genes for the beta subunit of human thyroid-stimulating hormone (TSH) and the glycoprotein hormone alpha subunit have been determined by restriction enzyme analysis of DNA extracted from rodent-human somatic cell hybrids. Human chorionic gonadotropin (CG) alpha-subunit cDNA and a cloned 0.9-kilobase (kb) fragment of the human TSH beta-subunit gene were used as hybridization probes in the analysis of Southern blots of DNA extracted from rodent-human hybrid clones. Analysis of the segregation of 5- and 10-kb EcoRI fragments hybridizing to CG alpha-subunit cDNA confirmed the previous assignment of this gene to chromosome 6. Analysis of the patterns of segregation of a 2.3-kb EcoRI fragment containing human TSH beta-subunit sequences permitted the assignment of the TSH beta-subunit gene to human chromosome 1. The subregional assignment of TSH beta subunit to chromosome 1p22 was made possible by the additional analysis of a set of hybrids containing partially overlapping segments of this chromosome. Human TSH beta subunit is not syntenic with genes encoding the beta subunits of CG, luteinizing hormone, or follicle-stimulating hormone and is assigned to a conserved linkage group that also contains the structural genes for the beta subunit of nerve growth factor (NGFB) and the proto-oncogene N-ras (NRAS).
D000818:Animals; D002417:Cattle; D002460:Cell Line; D006063:Chorionic Gonadotropin / Q000235:genetics; D002874:Chromosome Mapping; D002900:Chromosomes, Human, 1-3; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000235:genetics; D004262:DNA Restriction Enzymes; D005347:Fibroblasts; D005819:Genetic Markers; D006801:Humans; D006822:Hybrid Cells; D007739:L Cells; D008545:Melanoma; D051379:Mice; D000090063:Proto-Oncogene Mas; D013972:Thyrotropin / Q000235:genetics*
D006063:Chorionic Gonadotropin; D005819:Genetic Markers; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D013972:Thyrotropin; D004247:DNA; D004262:DNA Restriction Enzymes