Benzodiazepine anticonvulsants accelerate and beta-carboline convulsants decelerate the kinetics of [35S]TBPS binding at the chloride ionophore.
The effect of flunitrazepam and DMCM was examined on the kinetics of [35S]t-butylbicyclophosphorothionate (TBPS) binding. The enhancing effect of flunitrazepam and the decreasing effect of DMCM on TBPS binding was due to non-equilibrium conditions. The anticonvulsant benzodiazepine increased both the association and dissociation rate constants. The convulsant beta-carboline had the opposite effect. Benzodiazepine receptor ligands might affect the convulsant TBPS sites parallel with opening/closing of the chloride ionophores.
D000818:Animals; D000927:Anticonvulsants / Q000494:pharmacology*; D001569:Benzodiazepines / Q000494:pharmacology*; D001643:Bridged Bicyclo Compounds / Q000378:metabolism*; D019086:Bridged Bicyclo Compounds, Heterocyclic; D001952:Bridged-Ring Compounds / Q000378:metabolism*; D002243:Carbolines / Q000494:pharmacology*; D002712:Chlorides / Q000378:metabolism*; D003292:Convulsants / Q000494:pharmacology*; D005445:Flunitrazepam / Q000494:pharmacology; D066298:In Vitro Techniques; D007476:Ionophores / Q000378:metabolism*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D011963:Receptors, GABA-A / Q000187:drug effects; D013570:Synaptic Membranes / Q000378:metabolism
D000927:Anticonvulsants; D001643:Bridged Bicyclo Compounds; D019086:Bridged Bicyclo Compounds, Heterocyclic; D001952:Bridged-Ring Compounds; D002243:Carbolines; D002712:Chlorides; D003292:Convulsants; D007476:Ionophores; D011963:Receptors, GABA-A; D001569:Benzodiazepines; C034818:methyl 6,7-dimethoxy-4-ethyl-beta-carboline-3-carboxylate; D005445:Flunitrazepam; C037476:tert-butylbicyclophosphorothionate