Characterization and immunogenicity of HSV-1 antigens obtained following zwitterionic detergent treatment.
A preparation was obtained from herpesvirus hominis type 1 (HSV-1) infected cells using the zwitterionic detergent, Empigen BB. The preparation was partially purified by ultracentrifugation over a cushion of 20% sucrose. Serological characterization by ELISA and immuno double diffusion, using both polyclonal and monoclonal antibodies shows this preparation to contain HSV glycoproteins, including gC, gD and gE. Immunization of Balb/c mice elicited serum antibody responses against both HSV-1 and HSV-2 viruses, complete protection against HSV-1 (strain WAL) and partial protection against HSV-2 (strain 333).
D000818:Animals; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral / Q000096:biosynthesis*; D000956:Antigens, Viral / Q000032:analysis / Q000276:immunology*; D003902:Detergents; D004797:Enzyme-Linked Immunosorbent Assay; D006561:Herpes Simplex / Q000517:prevention & control; D007114:Immunization; D005779:Immunodiffusion; D051379:Mice; D008807:Mice, Inbred BALB C; D009930:Organic Chemicals; D018139:Simplexvirus / Q000276:immunology*; D014461:Ultracentrifugation; D014759:Viral Envelope Proteins / Q000032:analysis
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D003902:Detergents; D009930:Organic Chemicals; D014759:Viral Envelope Proteins; C039447:glycoprotein D, Human herpesvirus 1; C044768:glycoprotein E, herpes simplex virus type 1; C038535:glycoprotein gC, herpes simplex virus type 1; C041772:empigen BB