Temperature-sensitive mutants of the Streptomyces plasmid pIJ702.
DNA from the Streptomyces plasmid pIJ702 was mutagenized in vitro using hydroxylamine and transformed into Streptomyces lividans. One plasmid with temperature-sensitive replication (pMT660) and one plasmid with a temperature-sensitive tyrosinase (pMT661) were isolated. The plasmid pMT661 contains a novel PstI restriction endonuclease site within the tyrosinase gene.
D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000235:genetics; D009154:Mutation; D010957:Plasmids; D013302:Streptomyces / Q000235:genetics*; D013696:Temperature; D014169:Transformation, Bacterial
D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes