title: Microtubule disassembly increases the number of opioid receptor binding sites in rat cerebrum membranes.
abstract: The role of microtubules in opioid receptor binding was studied by using microtubule assembly inhibitors. Preincubation of rat cerebrum membranes with podophyllotoxin or colchicine provoked a marked increase in the number of binding sites as judged by [3H]-naloxone, [3H]-morphine and [3H]-D-Ala2-Leu5-enkephalin binding experiments. These results indicate microtubule involvement in regulation of opioid receptor expression.
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism* / Q000648:ultrastructure; D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure; D004090:Dihydromorphine / Q000378:metabolism; D004744:Enkephalin, Methionine / Q000031:analogs & derivatives / Q000378:metabolism; D007700:Kinetics; D007981:Levorphanol / Q000494:pharmacology; D008870:Microtubules / Q000378:metabolism* / Q000648:ultrastructure; D009270:Naloxone / Q000378:metabolism / Q000494:pharmacology; D011034:Podophyllotoxin / Q000494:pharmacology; D051381:Rats; D011957:Receptors, Opioid / Q000187:drug effects / Q000378:metabolism*
chemical_list: D011957:Receptors, Opioid; D007981:Levorphanol; D009270:Naloxone; D004744:Enkephalin, Methionine; C022118:enkephalinamide-Met, Ala(2)-; D004090:Dihydromorphine; D011034:Podophyllotoxin