[Ultrahistochemistry of cardiomyocytes in acquired mitral lesions of the heart].
A study of myocardium of patients with mitral stenosis was performed using ultrahistochemical methods at different stages of disease and in the course of valve replacement operation. A quantitative analysis of degenerative changes was introduced, the results of which correlated with the severity of heart failure. A pronounced heterogeneity in mitochondrial enzyme activity has been detected cytochemically. In some apparently "unaffected" cardiomyocytes an active alterative process was expressed in an increase of sarcolemma permeability for electron-microscopic tracers. An elevated fenestration of membrane barriers was noted at the stages of anoxic heart arrest. Using tannic acid it was possible to demonstrate a penetration of plasma protein-polysaccharide complexes into cell sarcoplasma. There were observed topographic variations in the distribution of acid phosphatase activity.
D000135:Acid Phosphatase / Q000378:metabolism; D002463:Cell Membrane Permeability; D003576:Electron Transport Complex IV / Q000378:metabolism; D006349:Heart Valve Diseases / Q000378:metabolism; D006651:Histocytochemistry; D006801:Humans; D008854:Microscopy, Electron; D008943:Mitral Valve; D009206:Myocardium / Q000378:metabolism*; D012214:Rheumatic Heart Disease / Q000378:metabolism*; D013385:Succinate Dehydrogenase / Q000378:metabolism
D013385:Succinate Dehydrogenase; D003576:Electron Transport Complex IV; D000135:Acid Phosphatase
Ul'tragistokhimiia kardiomiotsitov pri priobretennykh mitral'nykh porokakh serdtsa.