Mapping human chromosomes in somatic cell hybrids using a low-copy-number repetitive sequence.
We have isolated a human-specific repetitive sequence with a copy number of several hundred (p11L26). Using Southern blots of EcoR1-digested DNA from somatic cell hybrids containing one or a few human chromosomes, band patterns specific for those chromosomes can be generated by hybridization with p11L26. Genomic copies of the sequence have also been mapped to subchromosomal regions using translocations. The probe offers a useful addition to the standard techniques for mapping human chromosomes in hybrid cell lines.
D000818:Animals; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D006224:Cricetinae; D004262:DNA Restriction Enzymes; D006801:Humans; D006822:Hybrid Cells; D051379:Mice; D012091:Repetitive Sequences, Nucleic Acid; D014960:X Chromosome; D014998:Y Chromosome
D004262:DNA Restriction Enzymes