title: Sequence-specific interaction of histones with the simian virus 40 enhancer region in vitro.
abstract: DNA fragments containing either one or both of the 72-base pair (bp) elements which constitute the SV40 enhancer and the three adjacent 21-bp repeats were associated with histone octomers from chicken erythrocytes in vitro. Both fragments formed complexes with electrophoretic mobilities of nucleosomes containing the appropriate length of DNA. Analysis of DNase I cutting of uniquely end-labeled complexes suggests that the fragment containing a single 72-bp element forms a positioned core particle. Control experiments show that positioning is not due to the 21-bp repeats or to end effects. The fragment with a tandem repeat of the 72-bp element also does not associate randomly with histones. The data are consistent with formation of a core particle on one or the other of the repeated enhancer sequences. We discuss possible functional consequences of such nucleosome positioning.
mesh_terms: D000818:Animals; D001482:Base Composition; D001483:Base Sequence; D002645:Chickens; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant / Q000378:metabolism; D004279:DNA, Viral / Q000378:metabolism*; D003850:Deoxyribonuclease I / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004742:Enhancer Elements, Genetic; D005809:Genes, Regulator; D006657:Histones / Q000378:metabolism*; D046911:Macromolecular Substances; D009707:Nucleosomes / Q000378:metabolism; D013539:Simian virus 40 / Q000235:genetics*
chemical_list: D004274:DNA, Recombinant; D004279:DNA, Viral; D006657:Histones; D046911:Macromolecular Substances; D009707:Nucleosomes; D004262:DNA Restriction Enzymes; D003850:Deoxyribonuclease I