[Cleavage of DNA adsorbed on the surface of phospholipid membranes by restrictases type II].
Cleavage of phage lambda DNA by restriction endonucleases in the presence of model phosphatidylcholine membranes was studied. Bsp1, Pst1 and Bam H1 were found to cleave DNA under these conditions to a considerably decreased extent. This effect does not result from irreversible inactivation of the enzymes or their direct interaction with the membranes. The most probable explanation of the membrane inhibitory effect is the change of DNA substrate properties resulting from its Mg2+-mediated binding to the membranes.
D000327:Adsorption; D010582:Bacteriophage lambda / Q000378:metabolism; D004247:DNA / Q000378:metabolism*; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D004279:DNA, Viral / Q000378:metabolism; D006868:Hydrolysis; D008051:Lipid Bilayers; D010743:Phospholipids
D004279:DNA, Viral; D008051:Lipid Bilayers; D010743:Phospholipids; D004247:DNA; D004262:DNA Restriction Enzymes
Rasshcheplenie DNK, adsorbirovannoĭ na poverkhnosti fosfolipidnykh membran, restriktazami tipa II.