title: The temperate B. subtilis phage phi 105 genome contains at least two distinct regions encoding superinfection immunity.
abstract: Two different PstI fragments of temperate phage phi 105 DNA are shown to confer superinfection immunity upon Bacillus subtilis when inserted into the multicopy cloning vector pE194 cop-6. The 2.3 kb PstI fragment I is located almost entirely within EcoRI fragment F and encompasses a region previously known to encode a repressor. The other fragment, PstI-E (4.3 kb) maps inside the EcoRI-B fragment, and allows an explanation of the clear-plaque phenotype of the deletion mutant phi 105DII:6c. The two regions can be distinguished functionally, since only the PstI fragment I product interacts with a specific phi 105 promoter-operator site.
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics*; D001435:Bacteriophages / Q000235:genetics* / Q000276:immunology; D002872:Chromosome Deletion; D004262:DNA Restriction Enzymes; D005814:Genes, Viral; D007109:Immunity; D010641:Phenotype; D010957:Plasmids; D013696:Temperature
chemical_list: D004262:DNA Restriction Enzymes