A new endonuclease recognizing the deoxynucleotide sequence CCNNGG from the cyanobacterium Synechocystis 6701.
A new sequence-specific endonuclease from the cyanobacterium Synechocystis species PCC 6701 has been purified and characterized. This enzyme, SecI, is unique in recognizing the nucleotide sequence: 5' -CCNNGG-3' 3' -GGNNCC-5' and cleaves it at the position indicated by the  symbol. Two other restriction endonucleases, SecII and SecIII, found in this organism are isoschizomers of MspI and MstII, respectively.
D001483:Base Sequence; D000458:Cyanobacteria / Q000201:enzymology*; D004247:DNA; D004262:DNA Restriction Enzymes / Q000302:isolation & purification / Q000378:metabolism*; D015252:Deoxyribonucleases, Type II Site-Specific; D013379:Substrate Specificity
D004247:DNA; D004262:DNA Restriction Enzymes; C047012:endodeoxyribonuclease SecI; D015252:Deoxyribonucleases, Type II Site-Specific