[Two loci in the genome of the transposable phage B39 of Pseudomonas aeruginosa affecting the process of integration. I. Study of the properties of phages with the Pde- phenotype and mapping of the rms site].
Mutants and recombinants of transposable Pseudomonas aeruginosa bacteriophage B39 with a specific phenotype Pde- (pleiotropic developmental effect) were studied. Pde- phages produce clear minute plaques on lawns of P. aeruginosa PAO1 and fail to grow in cells of PAO1 harbouring Rms 163 (Inc P5) plasmid. Pde+ character is under control of the two loci in phage genome which were designated pdeX and pdeY. In hybrid phages the pdeX and pdeY loci originating from different transposable phages (pdeX from B39 and pdeY from PH132) do not accomplish their function and, as a result, the hybrid phages have the Pde- phenotype. The frequency of integration (f.o.i.) of Pde- phages into bacterial chromosome is lower than f.o.i. for Pde+ phages, as well as the frequency of stable lysogenization of infected bacteria; lytic development of the Pde- phages is also limited. The great difference among the transposable phages in their reaction to the presence of Rms163 plasmid is caused by some differences in the specific rms site in the phage genome. The site is located inside the interval 1.1-3.9 kb of the physical genome map, being closely linked to cI gene of phage B39. The growth of Pde- phages in cells with Rms163 can be restored, due to additional mutations in phage genes affecting lysogenization.
D001435:Bacteriophages / Q000235:genetics* / Q000254:growth & development; D002874:Chromosome Mapping; D004251:DNA Transposable Elements; D005814:Genes, Viral; D008242:Lysogeny; D009154:Mutation; D010641:Phenotype; D010957:Plasmids; D011550:Pseudomonas aeruginosa / Q000235:genetics
D004251:DNA Transposable Elements
Dva lokusa v genome faga-transpozona B39 Pseudomonas aeruginosa, vliiaiushchie na protsess integratsii. Soobshchenie I. Izuchenie svoĭstv fagov s fenotipom Pde- i kartirovanie saĭta rms.