title: Role of adenylate cyclase in human T-lymphocyte surface antigen capping.
abstract: Our recent studies indicated that capping of T3, T4 and T8 surface antigens on human T lymphocytes is augmented by interaction of adenosine with a purinergic receptor. We suggested that the T-cell capping process was mediated by an adenylate cyclase-coupled purinergic receptor that resulted in the generation of cAMP and occupancy of cAMP receptors. The present study was undertaken to examine whether activation of adenylate cyclase in the absence of purinergic stimulation is sufficient to regulate surface antigen capping. Treatment of T lymphocytes with forskolin or cholera toxin caused activation of adenylate cyclase and occupancy of intracellular types I and II regulatory subunits of protein kinase by cAMP, as demonstrated by photoaffinity labeling with [8-3H]N3-cAMP. Such treatment augmented the rate of capping of the T3, T4, and T8 antigens, which resulted in a significant decrement in the elapsed time to half-maximal capping of each antigen. These observations support the proposition that the normal T-lymphocyte capping mechanism of both T3+, T4+ (inducer/helper) and T3+, T8+ (suppressor) subsets can be augmented by activation of adenylate cyclase.
mesh_terms: D000241:Adenosine / Q000378:metabolism; D000262:Adenylyl Cyclases / Q000378:metabolism / Q000502:physiology*; D000328:Adult; D000954:Antigens, Surface / Q000276:immunology*; D002462:Cell Membrane / Q000201:enzymology; D002772:Cholera Toxin / Q000494:pharmacology; D005576:Colforsin / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D005260:Female; D019204:GTP-Binding Proteins / Q000502:physiology; D006801:Humans; D007152:Immunologic Capping / Q000187:drug effects; D008297:Male; D011956:Receptors, Cell Surface / Q000502:physiology; D011953:Receptors, Cyclic AMP / Q000378:metabolism; D011983:Receptors, Purinergic; D013601:T-Lymphocytes / Q000201:enzymology* / Q000276:immunology / Q000378:metabolism
chemical_list: D000954:Antigens, Surface; D011956:Receptors, Cell Surface; D011953:Receptors, Cyclic AMP; D011983:Receptors, Purinergic; D005576:Colforsin; D002772:Cholera Toxin; D019204:GTP-Binding Proteins; D000262:Adenylyl Cyclases; D000241:Adenosine