An alteration in tubulin expression detected in SV40 transformed 3T3 cells.
Tubulin expression was analysed in normal and simian virus-40 (SV40) transformed 3T3 cells by two-dimensional polyacrylamide gel electrophoresis and immunoblotting studies using monoclonal antibodies raised to alpha- and beta-tubulin subunits. The ratio of alpha- to beta-tubulin recognised was calculated for both cell lines and found to shift from 2.50 in normal cells to 0.52 in virally transformed cells. beta-Tubulin was thereby shown to be the predominant subunit in SV40-transformed 3T3 cells in contrast to normal 3T3 cells.
D000818:Animals; D000911:Antibodies, Monoclonal; D002460:Cell Line; D002472:Cell Transformation, Viral; D007163:Immunosorbent Techniques; D007526:Isoelectric Point; D051379:Mice; D008970:Molecular Weight; D013539:Simian virus 40; D014404:Tubulin / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D014404:Tubulin