Simplified preparation of unidirectional deletion clones.
D002872:Chromosome Deletion; D003001:Cloning, Molecular; D004720:Endonucleases; D005818:Genetic Engineering / Q000379:methods; D015719:Single-Strand Specific DNA and RNA Endonucleases
D004720:Endonucleases; D015719:Single-Strand Specific DNA and RNA Endonucleases