Viral isolation versus immune staining of infected cell cultures for the laboratory diagnosis of herpes simplex virus infections.
The Difco Laboratories Cellmatics kit, an immune staining method for the detection of herpes simplex virus after amplification in culture, was found to be 94.6% (138 of 146) sensitive and 100% (302 of 302) specific. However, conventional culturing detected 10 (6.4%) additional viruses that were not herpes simplex. For a full-service diagnostic laboratory, viral isolation is important.
D000818:Animals; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D006561:Herpes Simplex / Q000175:diagnosis*; D006801:Humans; D007124:Immunoenzyme Techniques; D011933:Reagent Kits, Diagnostic; D018139:Simplexvirus / Q000302:isolation & purification* / Q000502:physiology; D014709:Vero Cells
D011933:Reagent Kits, Diagnostic