The effect of dinitrophenol on magnesium transport across an isolated preparation of sheep rumen epithelium.
Mg transport across an isolated preparation of sheep rumen epithelium was studied in vitro. The efflux (flux from the mucosal side to serosal side) and the influx (flux with serosal to mucosal side) were estimated with 28Mg. Under physiological conditions a significant net efflux of Mg was observed, in which the unidirectional efflux was seven to eight times that of the influx. In experiments where ATP synthesis was blocked by the addition of dinitrophenol, Mg efflux was reduced to that of the influx. These observations support the conclusion that Mg absorption across the rumen wall of the sheep is an active process.
D000255:Adenosine Triphosphate / Q000096:biosynthesis; D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D004140:Dinitrophenols / Q000494:pharmacology*; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism; D066298:In Vitro Techniques; D008274:Magnesium / Q000378:metabolism*; D012417:Rumen / Q000187:drug effects* / Q000378:metabolism; D012756:Sheep; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism
D004140:Dinitrophenols; D000255:Adenosine Triphosphate; D000254:Sodium-Potassium-Exchanging ATPase; D008274:Magnesium