Increased atrial natriuretic peptide (6-33) binding sites in the subfornical organ of water deprived and Brattleboro rats.
Binding sites for rat atrial natriuretic peptide (6-33) (ANP) were quantitated in the subfornical organ of chronically dehydrated homozygous Brattleboro rats unable to synthesize vasopressin; heterozygous Brattleboro rats, their controls, Long Evans rats and Long Evans rats after 4 days of water deprivation. Brain sections were incubated in the presence of 125I-ANP and the results analyzed by autoradiography coupled to computerized microdensitometry and comparison to 125I-standards. Brattleboro rats and water deprived Long Evans rats presented a higher number of ANP binding sites than their normally hydrated controls. Our results suggest a role of ANP binding sites in the subfornical organ in the central regulation of fluid balance and vasopressin secretion.
D000818:Animals; D009320:Atrial Natriuretic Factor / Q000378:metabolism*; D001345:Autoradiography; D003681:Dehydration / Q000378:metabolism; D005625:Frontal Lobe / Q000378:metabolism*; D008297:Male; D010446:Peptide Fragments; D051381:Rats; D011910:Rats, Brattleboro; D017461:Receptors, Atrial Natriuretic Factor; D011956:Receptors, Cell Surface / Q000032:analysis*; D014667:Vasopressins / Q000378:metabolism; D014868:Water Deprivation / Q000502:physiology*; D014882:Water-Electrolyte Balance
D010446:Peptide Fragments; D011956:Receptors, Cell Surface; D014667:Vasopressins; D009320:Atrial Natriuretic Factor; C050513:atrial natriuretic factor prohormone (6-33), rat; D017461:Receptors, Atrial Natriuretic Factor