title: Structure and chromosomal localization of the human lymphotoxin gene.
abstract: We have isolated, sequenced, and determined the chromosomal localization of the gene encoding human lymphotoxin (LT). The single copy gene was isolated from a human genomic library using a 32P-labeled 116 bp synthetic DNA fragment whose sequence was based on the NH2-terminal amino acid sequence of LT. The gene spans 3 kb of DNA and is interrupted by three intervening sequences. The LT gene is located on human chromosome 6, as determined by Southern blot analysis of human-murine hybrid DNA. Putative transcriptional control regions and areas of homology with the promoters of interferon and other genes are identified.
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D002906:Chromosomes, Human, 6-12 and X; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D005796:Genes; D006801:Humans; D008233:Lymphotoxin-alpha / Q000235:genetics*; D011061:Poly A / Q000235:genetics; D011401:Promoter Regions, Genetic; D012315:RNA Caps / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid
chemical_list: D008233:Lymphotoxin-alpha; D012315:RNA Caps; D011061:Poly A; D004262:DNA Restriction Enzymes