Immunoblotting and enzyme-linked immunosorbent assay analysis of serological responses in patients infected with herpes simplex virus types 1 and 2.
Serological responses to soluble membrane and cytoplasmic antigens specified by herpes simplex type 1 (HSV1) and 2 (HSV2) were studied by immunoblotting and by enzyme-linked immunosorbent assay (ELISA). In the immunoblotting test, polypeptides migrating like the HSV1-specified glycoprotein C showed type-specific reactivity but could not always be detected. The immunoblotting and ELISA results were in agreement when antibody responses to HSV1- and HSV2-specified antigens were compared, and they allowed the identification of patients with HSV2 and/or HSV1 antibodies.
D000914:Antibodies, Viral / Q000032:analysis*; D000918:Antibody Specificity; D000954:Antigens, Surface / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006561:Herpes Simplex / Q000276:immunology*; D007163:Immunosorbent Techniques; D018139:Simplexvirus / Q000276:immunology*; D013045:Species Specificity
D000914:Antibodies, Viral; D000954:Antigens, Surface; D000956:Antigens, Viral