Complementation of an Escherichia coli pyrF mutant with DNA from Desulfovibrio vulgaris.
A PyrF- mutant of Escherichia coli (SK1108, pyrF::Tn5 Kanr) was complemented with the Desulfovibrio vulgaris (Hildenborough) structural gene for orotidine-5'-phosphate decarboxylase (EC 4.1.1.23). Either orientation of a 1.6-kilobase-pair D. vulgaris DNA fragment (pLP3B or pLP3A) complemented the PyrF- strain suggesting that the D. vulgaris pyrF promoter was functional. The apparent product of the D. vulgaris pyrF gene was a single 26-kilodalton polypeptide. These results demonstrate the utility of E. coli cloning systems in studying metabolic and energetic pathways in sulfate-reducing bacteria.
D002262:Carboxy-Lyases / Q000235:genetics*; D002874:Chromosome Mapping; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000235:genetics; D003901:Desulfovibrio / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D009964:Orotidine-5'-Phosphate Decarboxylase / Q000235:genetics*; D014170:Transformation, Genetic
D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes; D002262:Carboxy-Lyases; D009964:Orotidine-5'-Phosphate Decarboxylase