title: Influence of hyperthermia and gamma radiation on ADP-ribosyl transferase, NAD+, and ATP pools in human mononuclear leukocytes.
abstract: Effects of hyperthermia (42.5 degrees C) and gamma radiation (30 Gy) on ADP-ribosyl transferase, NAD+, and ATP pools in human mononuclear leukocytes have been investigated. It was found that the gamma-ray activation level of the enzyme was not influenced by this hyperthermia for 45 min. Following deprivation of ATP synthesis by 2,4-dinitrophenol, an uncoupler of the oxidative phosphorylation, and omitting glucose from the culture medium, the NAD+ pool was reduced to about 60% of control value. The potentiation of ATP production by exogenously supplied adenosine was reduced after a combined treatment of the cells with hyperthermia and gamma radiation. Mitochondrial and endoplasmic changes within the mononuclear leukocytes were also observed. Based on these findings a model for the hyperthermia effect is proposed.
mesh_terms: D019297:2,4-Dinitrophenol; D000241:Adenosine / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism*; D003080:Cold Temperature; D004140:Dinitrophenols / Q000494:pharmacology; D005720:Gamma Rays; D006801:Humans; D066298:In Vitro Techniques; D008854:Microscopy, Electron; D009000:Monocytes / Q000378:metabolism / Q000528:radiation effects*; D009243:NAD / Q000378:metabolism*; D009713:Nucleotidyltransferases / Q000378:metabolism*; D010085:Oxidative Phosphorylation / Q000187:drug effects; D011065:Poly(ADP-ribose) Polymerases; D013997:Time Factors
chemical_list: D004140:Dinitrophenols; D009243:NAD; D000255:Adenosine Triphosphate; D011065:Poly(ADP-ribose) Polymerases; D009713:Nucleotidyltransferases; D000241:Adenosine; D019297:2,4-Dinitrophenol