Globoseries glycosphingolipids of human meconium.
Globoside and an extended globoseries glycosphingolipid with a blood group H determinant were isolated from pooled human meconia and structurally characterized by mass spectrometry, proton NMR spectroscopy, and degradational techniques using GC and GC-MS analyses. Both species contained mainly phytosphingosine and hydroxy fatty acids characteristic for human intestinal epithelial cells. With the same techniques also minor amounts of globoside with sphingosine and nonhydroxy fatty acids and a novel globoseries tetraglycosyl ceramide with a terminal N-acetylglucosamine were isolated and structurally characterized.
D000107:Acetylation; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D005915:Globosides / Q000032:analysis*; D006028:Glycosphingolipids / Q000032:analysis*; D006801:Humans; D007231:Infant, Newborn; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008470:Meconium / Q000032:analysis*; D008745:Methylation; D011522:Protons
D005915:Globosides; D006028:Glycosphingolipids; D011522:Protons