title: Substrate phosphoprotein availability regulates eclosion hormone sensitivity in an insect CNS.
abstract: The final step in the moulting of all insects is ecdysis, the shedding of the cuticle of the previous instar, which is triggered in Lepidoptera by the neurosecretory peptide eclosion hormone. This hormone acts directly on the nervous system to release the appropriate motor patterns for larval, pupal and adult ecdysis, but there are only brief periods near the end of each moult when the nervous system is competent to respond to the hormone. Previous experiments have shown that the action of eclosion hormone on the nervous system at pupal ecdysis in the tobacco hornworm, Manduca sexta, is mediated by the second messenger cyclic GMP. Here we report that the hormone-stimulated increase in cGMP results in the phosphorylation of two proteins, each with an apparent relative molecular mass (Mr) of 54,000. Moreover, the brief periods during which the central nervous system (CNS) is responsive to eclosion hormone seem to result from the transient presence of these substrate proteins within the nervous system. This provides a novel mechanism by which hormonal responsiveness can be regulated.
mesh_terms: D000367:Age Factors; D000818:Animals; D002490:Central Nervous System / Q000254:growth & development; D006152:Cyclic GMP / Q000502:physiology*; D007301:Insect Hormones / Q000502:physiology*; D007526:Isoelectric Point; D007814:Larva; D007915:Lepidoptera / Q000254:growth & development*; D008970:Molecular Weight; D009036:Moths / Q000254:growth & development*; D010750:Phosphoproteins / Q000502:physiology*; D011494:Protein Kinases / Q000502:physiology
chemical_list: D007301:Insect Hormones; D010750:Phosphoproteins; D011494:Protein Kinases; D006152:Cyclic GMP