A transcribed satellite DNA from the bullfrog Rana catesbeiana.
We have cloned and sequenced a 360 bp repeated sequence from genomic DNA of the bullfrog Rana catesbeiana. This sequence, which we call satellite 1, makes up 0.7% of the genome (1.6 X 10(5) repeats) and is scattered throughout the length of all the chromosomes. In situ hybridization with strand-specific probes demonstrated that transcripts from both strands of satellite 1 occur on lamp-brush chromosome loops. We suggest that these transcripts arise by readthrough from upstream structural gene promoters. Sequences that cross-react with satellite 1 were found in genomic DNA of four out of five other Rana species that we tested, but were absent from the DNA of Xenopus, Bombina, and Acris.
D000818:Animals; D001482:Base Composition; D001483:Base Sequence; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004276:DNA, Satellite / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D011892:Rana catesbeiana / Q000235:genetics*; D011898:Ranidae / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid; D013045:Species Specificity; D014158:Transcription, Genetic
D004276:DNA, Satellite; D004262:DNA Restriction Enzymes