Identification of the human papilloma virus-1a E4 gene products.
Antibodies prepared against a human papilloma virus-1 (HPV-1) E4/beta-galactosidase fusion protein identified several polypeptides in HPV-1, but not HPV-2 or 4, induced papillomas. The major E4 protein, that represented up to 30% of total cellular protein, was a 16/17-K doublet which was purified by column chromatography and analysed for amino acid content. A peptide derived by chymotryptic digestion was purified by h.p.l.c. and subjected to amino acid sequencing. The unique sequence obtained, Gly-His-Pro-Asp-Leu-Ser-Leu, identified the 16/17-K doublet as a product of the HPV-1 E4 gene region. Antibodies to both the E4/beta-galactosidase fusion protein and the 16/17-K doublet identified two smaller polypeptides (10/11-K) which may represent spliced products of E4. We propose that the products of the HPV-1 E4 gene region are not classical DNA tumor virus early proteins and suggest that they play a role in virus maturation.
D000595:Amino Acid Sequence; D001483:Base Sequence; D005455:Fluorescent Antibody Technique; D005796:Genes; D005814:Genes, Viral; D006801:Humans; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D027383:Papillomaviridae / Q000235:genetics*; D010957:Plasmids; D014764:Viral Proteins / Q000032:analysis*; D014860:Warts / Q000382:microbiology; D001616:beta-Galactosidase / Q000235:genetics
D014764:Viral Proteins; D001616:beta-Galactosidase