[Deletion of late genes of the prophage lambda].
The deletions in tandem prophage lambda appear with high frequency (to 10%) in rec A- strain of Escherichia coli. The deletions were shown by marker rescue and hybridization of fragments of DNA on nitrocellulose filters with nick-translated phage lambda DNA localized only in prophage area. Right and left att sites are not involved. The majority of defective lysogens had all regulatory regions and deletions of late structural genes. These strains may be used for construction of the host-vector systems with the strongest promoter p'R of phage lambda.
D010582:Bacteriophage lambda / Q000235:genetics*; D002872:Chromosome Deletion; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005814:Genes, Viral; D005819:Genetic Markers; D005822:Genetic Vectors; D008242:Lysogeny; D009154:Mutation; D009693:Nucleic Acid Hybridization; D011401:Promoter Regions, Genetic
D004279:DNA, Viral; D005819:Genetic Markers; D004262:DNA Restriction Enzymes
Deletsiia pozdnikh genov profaga lambda.