Extramitochondrial citrate synthase activity in bakers' yeast.
We isolated the gene for citrate synthase (citrate oxaloacetate lyase; EC 4.1.3.7) from Saccharomyces cerevisiae and ablated it by inserting the yeast LEU2 gene within its reading frame. This revealed a second, nonmitochondrial citrate synthase. Like the mitochondrial enzyme, this enzyme was sensitive to glucose repression. It did not react with antibodies against mitochondrial citrate synthase. Haploid cells lacking a gene for mitochondrial citrate synthase grew somewhat slower than wild-type yeast cells, but exhibited no auxotrophic growth requirements.
D002950:Citrate (si)-Synthase / Q000235:genetics*; D004262:DNA Restriction Enzymes; D005796:Genes; D005800:Genes, Fungal; D008928:Mitochondria / Q000201:enzymology; D009693:Nucleic Acid Hybridization; D007652:Oxo-Acid-Lyases / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development
D002950:Citrate (si)-Synthase; D004262:DNA Restriction Enzymes; D007652:Oxo-Acid-Lyases