Production of human monoclonal IgG and IgM antibodies with anti-D (rhesus) specificity using heterohybridomas.
Heterohybridomas secreting human IgM and IgG anti-D antibodies of the rhesus blood group system have been established by fusion of EBV-transformed anti-D secreting cells with the mouse myeloma cells X63-Ag8.653. Both classes of antibody reacted with all Rh-positive cells, some Du cells but not with Rh-negative or DB cells. Concentrations of both antibodies reached between 25 micrograms/ml and 50 micrograms/ml in the culture supernatants. The cell lines have been maintained in culture for 14 months and have been shown to be suitable for large-scale production of antibody.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D002460:Cell Line; D002472:Cell Transformation, Viral; D006384:Hemagglutination; D004854:Herpesvirus 4, Human; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007074:Immunoglobulin G / Q000096:biosynthesis*; D007075:Immunoglobulin M / Q000096:biosynthesis*; D007136:Immunoglobulins / Q000096:biosynthesis*; D007621:Karyotyping; D051379:Mice; D009101:Multiple Myeloma / Q000473:pathology; D018029:Rho(D) Immune Globulin
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007136:Immunoglobulins; D018029:Rho(D) Immune Globulin