In situ hybridization with biotinylated DNA probes: a rapid diagnostic test for adenovirus upper respiratory infections.
Adenovirus DNA was detected in cells from nasopharyngeal secretions of children with acute respiratory infections by in situ hybridization with biotinylated probes. The technique was easy to perform, giving rapid results which were well correlated with those of immunofluorescence assays of the same samples. Adenoviruses of subgroups B, C and E were detected equally well by probes prepared either from purified adenovirus type 5 or from a plasmid (A1) carrying a cloned insertion of BamH1 fragments C and D of adenovirus type 2 in pAT 153. The use of stable non-radioactive probes makes in situ hybridization a feasible assay for use in clinical laboratories with moderate resources.
D000257:Adenoviridae Infections / Q000175:diagnosis*; D000258:Adenovirus Infections, Human / Q000175:diagnosis*; D000260:Adenoviruses, Human / Q000235:genetics / Q000302:isolation & purification*; D000818:Animals; D001710:Biotin; D002460:Cell Line; D002478:Cells, Cultured; D002648:Child; D004279:DNA, Viral / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D006801:Humans; D008253:Macaca mulatta; D009305:Nasopharynx / Q000378:metabolism / Q000382:microbiology; D009693:Nucleic Acid Hybridization; D012141:Respiratory Tract Infections / Q000175:diagnosis*
D004279:DNA, Viral; D001710:Biotin