Re-evaluation of a method calculating cleavage rates at different sites of DNA from partial digestion of end-labelled molecule.
Nucleases cleave the same targets at different rates in different parts of DNA molecules. The cleavage rates can be calculated from the amounts of particular fragments occurring in the DNA partial digests, but these are very complex and difficult to separate. To simplify the analysis, the use of end-labelled DNA molecules was suggested by Lutter. It is shown in the present communication that the fundamental equation of Lutter's approach is erroneous. A correct equation is derived and recommended to be used for re-calculation of the results obtained previously.
D004247:DNA; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D007700:Kinetics; D008433:Mathematics; D011865:Radioisotope Dilution Technique
D004247:DNA; D004262:DNA Restriction Enzymes