Detection of mucus glycoconjugates in human conjunctiva by using the lectin-colloidal gold technique in TEM. II. A quantitative study in dry-eye patients.
The mucus glycoconjugates produced by conjunctival goblet cells in dry-eye patients were studied by a specific cytochemical reaction in Transmission Electron Microscopy (TEM). Four lectins, proteins of vegetal origin which specifically bind glycosidic residues, (WGA, PNA, SBA and ConA) were used conjugated with colloidal gold as ultrastructural marker. We performed a quantitative analysis by counting the colloidal gold particles present on mucus granules. The results were compared with normal conditions. We found a decrease in sialic acid, N-acetyl-glucosamine, N-acetyl-galactosamine and galactose-N-acetyl-galactosamine and an increase in mannose. The different content of glycoconjugates in goblet cells may reflect in the change of physical and functional properties of mucus. We think these data may be useful in the search for a therapeutic mucomimetic drug.
D050260:Carbohydrate Metabolism; D003208:Concanavalin A; D003228:Conjunctiva / Q000378:metabolism* / Q000648:ultrastructure; D005260:Female; D006023:Glycoproteins / Q000378:metabolism*; D006048:Gold Colloid, Radioactive; D006651:Histocytochemistry; D006801:Humans; D007637:Keratoconjunctivitis / Q000378:metabolism*; D007638:Keratoconjunctivitis Sicca / Q000378:metabolism* / Q000473:pathology; D037102:Lectins; D008297:Male; D008854:Microscopy, Electron / Q000379:methods; D008875:Middle Aged; D009093:Mucus / Q000378:metabolism*; D019887:Peanut Agglutinin; D037121:Plant Lectins; D030262:Soybean Proteins; D014909:Wheat Germ Agglutinins; D010835:Phytohemagglutinins
D003208:Concanavalin A; D006023:Glycoproteins; D006048:Gold Colloid, Radioactive; D037102:Lectins; D019887:Peanut Agglutinin; D037121:Plant Lectins; D030262:Soybean Proteins; D014909:Wheat Germ Agglutinins; C028824:soybean lectin; D010835:Phytohemagglutinins