Synthesis and in vitro activity of a hormone-diphtheria toxin fragment A hybrid.
The synthesis and in vitro biological activity of a hybrid protein composed of intact human chorionic gonadotropin and fragment A of diphtheria toxin in a disulfide conjugate is reported. This hybrid retained greater than 90% of the binding ability of uncoupled hCG and was shown to be specifically toxic to a mouse Leydig cell tumor which binds hCG while being non-toxic towards cells which lack receptors for hCG.
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D006063:Chorionic Gonadotropin / Q000138:chemical synthesis*; D002846:Chromatography, Affinity; D004167:Diphtheria Toxin / Q000138:chemical synthesis*; D004591:Electrophoresis, Polyacrylamide Gel; D007984:Leydig Cell Tumor / Q000378:metabolism; D051379:Mice; D011956:Receptors, Cell Surface / Q000378:metabolism; D011974:Receptors, LH; D011993:Recombinant Fusion Proteins
D006063:Chorionic Gonadotropin; D004167:Diphtheria Toxin; D011956:Receptors, Cell Surface; D011974:Receptors, LH; D011993:Recombinant Fusion Proteins; C047471:human chorionic gonadotropin-diphtheria toxin fragment A