Inactivation of peroxidase and glucose oxidase by H2O2 and iodide during in vitro thyroglobulin iodination.
Thyroglobulin iodination and thyroxine synthesis in vitro require the presence of peroxidase, H2O2 and iodide. H2O2 is usually continuously generated by glucose oxidase (GO) and glucose. The aim of this study was to investigate whether the two enzymes could possibly be inactivated by a particular concentration of H2O2 or iodide present during incubation. The results revealed that both enzymes were indeed inactivated under two distinct conditions: Lactoperoxidase and thyroid peroxidase were inactivated by modest concentrations of H2O2 accumulating during incubation. Glucose oxidase was inactivated by an oxidized species of iodine or singlet oxygen produced in the catalytic cycle. The results may explain some hitherto unsolved discrepancies between different iodination procedures. Moreover they may have an impact on the regulation of in vivo thyroglobulin iodination and hormone synthesis.
D005949:Glucose Oxidase / Q000037:antagonists & inhibitors*; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D066298:In Vitro Techniques; D007453:Iodide Peroxidase / Q000037:antagonists & inhibitors; D007454:Iodides / Q000494:pharmacology*; D007455:Iodine / Q000378:metabolism; D007527:Isoenzymes / Q000037:antagonists & inhibitors*; D007700:Kinetics; D007784:Lactoperoxidase / Q000037:antagonists & inhibitors; D009195:Peroxidase; D010544:Peroxidases / Q000037:antagonists & inhibitors*; D013954:Thyroglobulin / Q000378:metabolism*
D007454:Iodides; D007527:Isoenzymes; D013954:Thyroglobulin; D007455:Iodine; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; D007784:Lactoperoxidase; D010544:Peroxidases; D009195:Peroxidase; D007453:Iodide Peroxidase