title: Location of cis-acting regulatory sequences in the human T-cell leukemia virus type I long terminal repeat.
abstract: The location of cis-acting regulatory regions within the long terminal repeat (LTR) of the human T-cell leukemia virus type I (HTLV-1) was determined. The sequences present between nucleotides -350 and -55 (cap site +1) contain an enhancer element that is active in lymphoid and nonlymphoid cell lines. The sequences located near the "TATA" and RNA initiation sites contain a promoter, the activity of which can be augmented by homologous and heterologous enhancer elements. A region responsive to trans-acting transcription factors present in HTLV-I- and HTLV type II-infected cells is located between nucleotides -159 and +315. HTLV-I LTR deletion mutants respond in a similar manner both to the trans-acting factors present in infected cells and to the tat protein encoded by the x-lor region of the genome, thus providing further evidence that the tat protein mediates transcriptional trans-activation of the LTR in HTLV-infected cells.
mesh_terms: D001358:Avian Sarcoma Viruses / Q000235:genetics; D002472:Cell Transformation, Viral; D003001:Cloning, Molecular; D017977:Deltaretrovirus / Q000235:genetics* / Q000502:physiology; D004742:Enhancer Elements, Genetic; D015696:Gene Products, tat; D005809:Genes, Regulator; D005813:Genes, Synthetic; D005814:Genes, Viral; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D013539:Simian virus 40 / Q000235:genetics; D014157:Transcription Factors / Q000502:physiology*; D014158:Transcription, Genetic; D014779:Virus Replication
chemical_list: D015696:Gene Products, tat; D014157:Transcription Factors