[Cloning of the DNA fragment of a transgenic mouse containing an integrated recombinant plasmid].
The "plasmid rescue" method has been used to isolate the recombinant plasmid pMA3 fragment and flanking sequences from the transgenic mouse genome containing the fragment in integrated form. The "rescued" plasmid, pMAR1, lacks all virus sequences and retains only those regions of pBR322 that are responsible for the plasmid replication and Escherichia coli ampicillin resistance. The plasmid pMA3 deletion has occurred at its integration into the mouse genome after microinjection into the zygote. The integrated fragment of the plasmid is adjacent to the genome repeated sequence that is highly conservative in evolution.
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL / Q000235:genetics; D009693:Nucleic Acid Hybridization; D010957:Plasmids
D004274:DNA, Recombinant; D004262:DNA Restriction Enzymes
Klonirovanie fragmenta DNK transgennoĭ myshi, soderzhashchego integrirovannuiu rekombinantnuiu plazmidu.