Studies on steroids. CCXIX. Separation and determination of 4-hydroxyoestriol monoglucuronides and monosulphates in biological fluids by high-performance liquid chromatography with electrochemical detection.
The separation and determination of 4-hydroxyoestriol monoglucuronides and monosulphates by high-performance liquid chromatography with electrochemical detection on a reversed-phase column has been carried out. The effects of the salt, composition and pH of the mobile phase on the resolution were investigated with a Develosil ODS-5 column. Each group of isomeric monoglucuronides and monosulphates of 4-hydroxyoestriol was efficiently resolved on this column when 0.5% sodium acetate-acetonitrile and 0.5% sodium acetate-tetrahydrofuran-acetonitrile were used as mobile phases, respectively. The use of the present method revealed that 4-hydroxyoestriol orally administered to the rat was excreted as 4-, 3-, 16-glucuronides and 4-sulphate in bile.
D000818:Animals; D001646:Bile / Q000032:analysis; D001826:Body Fluids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D004964:Estriol / Q000031:analogs & derivatives* / Q000302:isolation & purification; D004966:Estrogens, Conjugated (USP) / Q000302:isolation & purification*; D005965:Glucuronates / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013464:Sulfuric Acids / Q000302:isolation & purification
D004966:Estrogens, Conjugated (USP); D005965:Glucuronates; D013464:Sulfuric Acids; C014038:4-hydroxyestriol; D004964:Estriol