title: Sendai virus-erythrocyte membrane interaction: quantitative and kinetic analysis of viral binding, dissociation, and fusion.
abstract: A kinetic and quantitative analysis of the binding and fusion of Sendai virus with erythrocyte membranes was performed by using a membrane fusion assay based on the relief of fluorescence self-quenching. At 37 degrees C, the process of virus association displayed a half time of 2.5 min; at 4 degrees C, the half time was 3.0 min. The fraction of the viral dose which became cell associated was independent of the incubation temperature and increased with increasing target membrane concentration. On the average, one erythrocyte ghost can accommodate ca. 1,200 Sendai virus particles. The stability of viral attachment was sensitive to a shift in temperature: a fraction of the virions (ca. 30%), attached at 4 degrees C, rapidly (half time, ca. 2.5 min) eluted from the cell surface at 37 degrees C, irrespective of the presence of free virus in the medium. The elution can be attributed to a spontaneous, temperature-induced release, rather than to viral neuraminidase activity. Competition experiments with nonlabeled virus revealed that viruses destined to fuse do not exchange with free particles in the medium but rather bind in a rapid and irreversible manner. The fusion rate of Sendai virus was affected by the density of the virus particles on the cell surface and became restrained when more than 170 virus particles were attached per ghost. In principle, all virus particles added displayed fusion activity. However, at high virus-to-ghost ratios, only a fraction actually fused, indicating that a limited number of fusion sites exist on the erythrocyte membrane. We estimate that ca. 180 virus particles maximally can fuse with one erythrocyte ghost.
mesh_terms: D003080:Cold Temperature; D004229:Dithiothreitol / Q000494:pharmacology; D004910:Erythrocyte Membrane / Q000378:metabolism* / Q000382:microbiology; D006461:Hemolysis; D006801:Humans; D007700:Kinetics; D008561:Membrane Fusion / Q000187:drug effects; D010222:Parainfluenza Virus 1, Human / Q000502:physiology*; D014759:Viral Envelope Proteins / Q000502:physiology; D014760:Viral Fusion Proteins
chemical_list: D014759:Viral Envelope Proteins; D014760:Viral Fusion Proteins; D004229:Dithiothreitol