[Chromatographic properties of human leukocyte alpha-interferon A on sorbents with sepharose and silochrome matrices].
Chromatography on immobilized monoclonal antibodies NK-2 from a bacterial strain-producer resulted in a pure human leukocyte alpha-interferon A (alpha-INF-A) homogeneous upon polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate and reverse phase high performance liquid chromatography. The chromatographic properties of partially purified alpha-INF-A on synthetic and commercial sorbents containing immobilized dyes, aromatic dipeptides, chelating and hydrophobic ligands as well as on porous glass have been investigated. In most cases, [125I]alpha-INF-A was used as an inner standard. The chromatographic behaviour of native and [125I]-labeled alpha-INF-A was practically the same. alpha-INF-A was most effectively chromatographed on porous glass, L-Trp-L-Trp-Sepharose 4B and Cu2+-chelate sorbents. In the latter case, the feasibility of substitution of the Sepharose matrix for the silochrome one has been demonstrated. It has been proposed that alpha-INF-A has a hydrophobic "pocket" with exposed aromatic amino acid residues which are capable of selective binding to aromatic dipeptides.
D002847:Chromatography, Agarose / Q000295:instrumentation; D006801:Humans; D007370:Interferon Type I / Q000032:analysis* / Q000302:isolation & purification; D012685:Sepharose; D058428:Silica Gel; D012822:Silicon Dioxide
D007370:Interferon Type I; D058428:Silica Gel; D012822:Silicon Dioxide; D012685:Sepharose
Khromatograficheskie svoĭstva chelovecheskogo leĭkotsitarnogo alpha-interferona A na sorbentakh s sefaroznymi i silokhromnymi svoĭstvami.