Resistance to antimycin A in yeast by amplification of ADH4 on a linear, 42 kb palindromic plasmid.
A yeast strain lacking a functional copy of ADH1 has been isolated that is resistant to antimycin A because of the presence of multiple copies of a nuclear gene, ADH4. The amplified copies of ADH4 exist on linear molecules 42 kb in length, which can be separated from chromosomal DNA by orthogonal-field-alternation gel electrophoresis. These amplified molecules are palindromes that reanneal rapidly after denaturation to form linear, snap-back molecules 21 kb in length. The amplified ADH4 sequences are bounded by telomere-homologous sequences. The chromosomal copy of ADH4 is the most distal marker on the left arm of chromosome VII, and the amplified ADH4-containing molecules appear to contain two copies of the region extending from ADH4 to the telomere.
D000426:Alcohol Dehydrogenase / Q000235:genetics*; D000968:Antimycin A / Q000494:pharmacology*; D001483:Base Sequence; D002874:Chromosome Mapping; D002875:Chromosomes / Q000648:ultrastructure; D004262:DNA Restriction Enzymes; D004271:DNA, Fungal / Q000235:genetics; D004352:Drug Resistance, Microbial; D004586:Electrophoresis / Q000379:methods; D005784:Gene Amplification; D009695:Nucleic Acid Renaturation; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D004271:DNA, Fungal; D000968:Antimycin A; D000426:Alcohol Dehydrogenase; D004262:DNA Restriction Enzymes