Fluidizing effects of centrophenoxine in vitro on brain and liver membranes from different age groups of mice.
This study examined the effects of different concentrations of centrophenoxine on physical properties of synaptic plasma membranes and liver microsomes using electron spin resonance procedures. Membranes of different age groups of mice were labeled with the 5-doxyl stearic acid spin-label and membrane fluidity determined in the presence and absence of different concentrations of centrophenoxine. Centrophenoxine had a direct effect on membranes as shown by a significant increase in membrane fluidity. This effect was greatest in liver microsomes as compared to synaptic plasma membranes. Age differences were not observed in centrophenoxine-induced fluidization. Effects of centrophenoxine in vivo may be due in part to the drug acting directly on the physical properties of the membrane lipid environment.
D000375:Aging / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000187:drug effects*; D004578:Electron Spin Resonance Spectroscopy; D006016:Glycolates / Q000494:pharmacology*; D066298:In Vitro Techniques; D002504:Meclofenoxate / Q000494:pharmacology*; D008560:Membrane Fluidity / Q000187:drug effects*; D051379:Mice; D008810:Mice, Inbred C57BL; D008862:Microsomes, Liver / Q000187:drug effects*; D013570:Synaptic Membranes / Q000187:drug effects
D006016:Glycolates; D002504:Meclofenoxate