Establishment of a mouse model for human rhinovirus infection.
We describe here a mouse model for rhinovirus infection using a variant of human rhinovirus type 2 (HRV2/H) which replicated 50- to 300-fold in the lungs of BALB/c mice. The variant virus differed only marginally from HRV2/H according to various biochemical parameters. Use of a photosensitive inoculum and pretreatment of the animals with actinomycin D were necessary for detection of reproducible and significant levels of virus replication. This mouse model of rhinovirus infection is the first example of human rhinovirus replication in a non-primate mammal, and provides an important link for the development of rhinovirus therapy or prophylaxis.
D000818:Animals; D003139:Common Cold / Q000382:microbiology*; D003609:Dactinomycin / Q000494:pharmacology; D004195:Disease Models, Animal / Q000382:microbiology*; D005260:Female; D008168:Lung / Q000382:microbiology; D051379:Mice; D008815:Mice, Inbred Strains; D012229:Rhinovirus / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D012376:Rodent Diseases / Q000382:microbiology; D014779:Virus Replication
D003609:Dactinomycin