Transformation of Ob17 cells promotes proliferation and differentiation of Ob17 preadipocytes via distinct extracellular intermediates.
Conditioned serum-free medium of Ob17 cells transformed by the middle-T-only gene of polyoma virus (Ob17MT cells) is able to support growth and adipose conversion of the parental Ob17 cells. Conditioned media from 3T3-F442A cells (untransformed preadipocyte clonal line) and MTT4 cells (middle-T-transformed non-preadipocyte clonal line) are inactive. The serum-free conditioned medium of Ob17MT cells is also active on growth and adipose conversion of 3T3-F442A cells. The morphological differentiation of Ob17 cells is accompanied by the expression of early (lipoprotein lipase, LPL) and late (glycerol-3-phosphate dehydrogenase, GPDH) biochemical markers of adipose conversion. Bio-Gel P-60 chromatography and SDS-PAGE have allowed characterization of a mitogenic fraction of apparent MW approximately equal to 28 Kd distinct from an adipogenic fraction of apparent MW less than 10 Kd. This adipogenic fraction is only required for the acquisition of the GPDH activity and is therefore active on terminal differentiation.
D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D000957:Antigens, Viral, Tumor / Q000235:genetics; D002453:Cell Cycle; D002454:Cell Differentiation; D002460:Cell Line; D002472:Cell Transformation, Viral; D003470:Culture Media; D004285:Dogs; D013006:Growth Hormone / Q000494:pharmacology; D011120:Polyomavirus / Q000235:genetics / Q000276:immunology; D014170:Transformation, Genetic; D014284:Triiodothyronine / Q000494:pharmacology
D000957:Antigens, Viral, Tumor; D003470:Culture Media; D014284:Triiodothyronine; D013006:Growth Hormone