Scanning spectrophotometry for the dynamic study of tissue respiration in intact organs.
For use with intact perfused organs a spectrophotometer system has been developed, both for dual-wavelength absorption measurements and for spectral scanning. A monochromator is used for illumination and scanning in the visible and near infrared. Optic fibres conduct light to the specimen under examination and from the specimen to a detecting photomultiplier. System control is exercised by a microcomputer, which also processes the collected data. The performance of the system on isolated perfused rat heart is demonstrated, in the spectral scanning mode and in the dual-wavelength mode, by studying simultaneously the kinetics of cytochrome aa3, and myoglobin oxidation-reduction.
D000818:Animals; D003626:Data Display; D003576:Electron Transport Complex IV / Q000378:metabolism; D007700:Kinetics; D008838:Microcomputers; D009206:Myocardium / Q000201:enzymology / Q000378:metabolism*; D009211:Myoglobin / Q000378:metabolism; D010084:Oxidation-Reduction; D010101:Oxygen Consumption; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains; D013053:Spectrophotometry
D009211:Myoglobin; D003576:Electron Transport Complex IV