A routine method for the establishment of permanent growing lymphoblastoid cell lines.
Permanent lymphoblastoid cell lines are of great practical value in human clinical and experimental genetics. A detailed protocol for routine use is given for the establishment of lymphoblastoid lines from peripheral blood using Epstein-Barr virus and the immunosuppressivum Cyclosporin A. In addition, the biologic basis of this transformation system is briefly summarized.
D000956:Antigens, Viral / Q000032:analysis; D001402:B-Lymphocytes / Q000166:cytology* / Q000382:microbiology / Q000648:ultrastructure; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002472:Cell Transformation, Viral; D002869:Chromosome Aberrations; D003524:Cyclosporins / Q000494:pharmacology; D019309:Epstein-Barr Virus Nuclear Antigens; D004996:Ethidium / Q000494:pharmacology; D004854:Herpesvirus 4, Human / Q000276:immunology; D006801:Humans; D007621:Karyotyping
D000956:Antigens, Viral; D003524:Cyclosporins; D019309:Epstein-Barr Virus Nuclear Antigens; D004996:Ethidium