Effect of oxidizing agents on rabbit mammary prolactin receptors.
Treatment of rabbit mammary membrane-bound and solubilized prolactin receptors with the oxidizing agents N-chlorobenzene sulfonamide and N-bromosuccinimide resulted in total inactivation of the ability of the receptor to bind prolactin. A similar inactivation was obtained with 2-hydroxy-5-nitrobenzylbromide. The results suggest the possible importance of tryptophan in maintaining the activity of the prolactin receptor.
D015077:2-Hydroxy-5-nitrobenzyl Bromide / Q000494:pharmacology*; D000818:Animals; D001974:Bromosuccinimide / Q000494:pharmacology*; D002700:Chloramines / Q000494:pharmacology*; D005260:Female; D007700:Kinetics; D008321:Mammary Glands, Animal / Q000378:metabolism*; D009596:Nitrophenols / Q000494:pharmacology*; D011388:Prolactin / Q000378:metabolism; D011817:Rabbits; D011956:Receptors, Cell Surface / Q000187:drug effects* / Q000378:metabolism; D011981:Receptors, Prolactin; D013388:Succinimides / Q000494:pharmacology*; D000096926:Benzenesulfonamides
D015077:2-Hydroxy-5-nitrobenzyl Bromide; D001974:Bromosuccinimide; D002700:Chloramines; D009596:Nitrophenols; D011388:Prolactin; D011956:Receptors, Cell Surface; D011981:Receptors, Prolactin; D013388:Succinimides; C030263:chloramine-B; D000096926:Benzenesulfonamides