Prolactin receptor: identification of the binding unit by affinity labelling and characterization of poly- and monoclonal antibodies.
The prolactin receptor localized in rabbit mammary gland membranes has been identified by affinity labelling using covalent cross-linking agents such as a unique protein chain of approximately 32,000 daltons. After partial purification (5,000-fold) of these receptors from mammary gland homogenate, polyclonal antibodies, which specifically and completely inhibit prolactin binding in all organs and in all species studied, were raised. These antibodies possessed prolactin-like biological activity (casein synthesis) on rabbit mammary gland explants. Monoclonal antibodies specifically directed against the binding domain of the receptor were also obtained. These antibodies were more species-specific than the polyclonal antibodies. The most potent (M110) possessed higher affinity than prolactin for the receptor and could be a very effective tool to elucidate the structure of the receptor and its immunological detection.
D000818:Animals; D000906:Antibodies; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex / Q000032:analysis; D002846:Chromatography, Affinity; D005260:Female; D007425:Intracellular Membranes / Q000378:metabolism; D007774:Lactation; D008297:Male; D008321:Mammary Glands, Animal / Q000378:metabolism; D008861:Microsomes / Q000378:metabolism; D008970:Molecular Weight; D011247:Pregnancy; D011388:Prolactin / Q000378:metabolism*; D011817:Rabbits; D011956:Receptors, Cell Surface / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D011981:Receptors, Prolactin; D014018:Tissue Distribution
D000906:Antibodies; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D011956:Receptors, Cell Surface; D011981:Receptors, Prolactin; D011388:Prolactin