Separation of nuclear cAMP independent protein kinases NI and NII from their chromosomal protein substrates and enzyme inhibitors by the use of a casein-phosvitin-Sepharose column.
A casein-phosvitin-Sepharose chromatography column allows separation of nuclear protein kinases from their chromosomal phosphoprotein substrates and from at least some protein kinase inhibitors in a single step. The additional step of passing the eluted material through a partially hydrolyzed, dephosphorylated casein-Sepharose column separates the two protein kinases, NI and NII, from each other.
D000818:Animals; D002364:Caseins; D002467:Cell Nucleus / Q000201:enzymology*; D002846:Chromatography, Affinity; D002868:Chromosomal Proteins, Non-Histone / Q000302:isolation & purification*; D003600:Cytosol / Q000201:enzymology; D006657:Histones; D006868:Hydrolysis; D008297:Male; D010749:Phosphoprotein Phosphatases / Q000032:analysis; D010774:Phosvitin; D011485:Protein Binding; D047428:Protein Kinase Inhibitors; D011494:Protein Kinases / Q000302:isolation & purification*; D051381:Rats; D011919:Rats, Inbred Strains; D012685:Sepharose
D002364:Caseins; D002868:Chromosomal Proteins, Non-Histone; D006657:Histones; D047428:Protein Kinase Inhibitors; D010774:Phosvitin; D012685:Sepharose; D011494:Protein Kinases; C036749:protein kinase NI; C026813:protein kinase NII; D010749:Phosphoprotein Phosphatases