Studies of protein-phospholipid interaction in isolated mitochondrial ubiquinone-cytochrome c reductase.
The interaction between phospholipids, ubiquinone and highly purified ubiquinol-cytochrome c reductase was studied using differential scanning calorimetry. The enzyme complex and its delipidated forms undergo thermodenaturation at 337.3 and 322.7 K, respectively. The reduced reductase is more stable toward thermodenaturation than is the oxidized enzyme. While phospholipids restored enzymatic activity to the delipidated enzyme complex and stabilized the enzyme toward thermodenaturation, ubiquinone showed little effect on the thermostability of ubiquinol-cytochrome c reductase. The effect of phospholipids on the thermotropic properties of ubiquinol-cytochrome c reductase is dependent upon the molecular properties of the phospholipid. When ubiquinol-cytochrome c reductase was embedded in closed asolectin vesicles, an exothermic transition peak was observed upon thermodenaturation. When the asolectin concentration in the reconstituted preparation was less than 0.3 mg/mg protein, an amorphous structure was observed in the electron micrograph and the preparation showed an endothermic transition upon thermodenaturation. The thermotropic properties of the enzyme-phospholipid vesicles were affected by the phospholipid head groups as well as the fatty-acyl chains, with those phospholipids having the most highly unsaturated fatty-acyl chains having the greatest effect. The energy for the exothermic transition may be derived from the collapse, upon thermodenaturation, of a strained interaction between the unsaturated fatty-acyl groups of phospholipids and protein molecules resulting from vesicle formation. The exothermic transition of the enzyme-phospholipid vesicle was abolished when cholesterol was included in the vesicles and when reductase was treated with a proteolytic enzyme prior to incorporation into the phospholipid vesicles.
D000818:Animals; D002152:Calorimetry, Differential Scanning; D002417:Cattle; D055598:Chemical Phenomena; D002621:Chemistry; D002784:Cholesterol / Q000494:pharmacology; D003574:Cytochrome c Group / Q000378:metabolism; D004579:Electron Transport; D014450:Electron Transport Complex III; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology; D005614:Freeze Fracturing; D006358:Hot Temperature; D008081:Liposomes; D008854:Microscopy, Electron; D008929:Mitochondria, Heart / Q000201:enzymology*; D009097:Multienzyme Complexes / Q000378:metabolism; D010447:Peptide Hydrolases / Q000378:metabolism; D010713:Phosphatidylcholines / Q000494:pharmacology; D010743:Phospholipids / Q000494:pharmacology; D011489:Protein Denaturation; D011808:Quinone Reductases / Q000378:metabolism; D013329:Structure-Activity Relationship; D013816:Thermodynamics; D014451:Ubiquinone / Q000494:pharmacology
D003574:Cytochrome c Group; D005231:Fatty Acids, Unsaturated; D008081:Liposomes; D009097:Multienzyme Complexes; D010713:Phosphatidylcholines; D010743:Phospholipids; D014451:Ubiquinone; C024990:asolectin; D002784:Cholesterol; D011808:Quinone Reductases; D010447:Peptide Hydrolases; D014450:Electron Transport Complex III