High-performance liquid chromatography using spherical aggregates of hydroxyapatite micro-crystals as adsorbent.
High-performance liquid chromatography (HPLC) using spherical aggregates of hydroxyapatite (HA) microcrystals as adsorbent has been developed; preliminary performance tests were carried out by using several types of protein. In comparison with previously developed plate-like HA packed columns for HPLC, spherical HA packed columns show considerably high chromatographic resolutions in spite of extremely reduced column lengths of 0.5-3 cm. The pressure generated by the latter columns is much higher than that generated by the former, however.
D000327:Adsorption; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003460:Crystallization; D003574:Cytochrome c Group / Q000302:isolation & purification; D003851:Deoxyribonucleases / Q000302:isolation & purification; D017886:Durapatite; D006882:Hydroxyapatites; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D009113:Muramidase / Q000302:isolation & purification; D010434:Pepsin A / Q000302:isolation & purification; D012473:Salmine / Q000302:isolation & purification; D014362:Trypsinogen / Q000302:isolation & purification; D014961:X-Ray Diffraction
D003574:Cytochrome c Group; D006882:Hydroxyapatites; D014362:Trypsinogen; D012473:Salmine; D017886:Durapatite; D003851:Deoxyribonucleases; D009113:Muramidase; D010434:Pepsin A