title: Superoxide anion generating capacity and lysosomal enzyme activities of Kupffer cells in galactosamine induced hepatitis.
abstract: To elucidate the function of the reticuloendothelial system of liver in hepatic injury, we investigated the effect of endotoxins on superoxide anion (O-2) generating capacity and lysosomal enzyme activities of Kupffer cells isolated from rats treated with galactosamine (Gal N), with Gal N supplemented with polymyxin B (Polymyxin B-Gal N), with lipopolysaccharide (LPS) and from control rats. After collagenase digestion of the liver and centrifugation over metrizamide gradient, Kupffer cells were prepared by the dish adherence procedure. O-2 production by the cells was examined as chemiluminescence during phagocytosis of latex particles and beta-glucuronidase activities were analyzed. High titers of endotoxemia were detected in LPS and Gal N rats by limulus test, while a low endotoxemia titer was found in Polymyxin B-Gal N rats. Hepatocyte damage was found in Gal N rats, but little was recognized in LPS and Polymyxin B-Gal N rats. In the latter groups, Kupffer cells, activated by endotoxins, showed the enhancement of chemiluminescence and a release of lysosomal enzyme. Though lysosomal enzyme was released from Kupffer cells in Gal N rats, chemiluminescence was slightly suppressed in spite of the high titer of endotoxemia. These results appear to be related to the consumption of O-2 during liver injury. The functional state of Kupffer cells was thus changed by the grade of endotoxemia and hepatic injury.
mesh_terms: D000818:Animals; D056486:Chemical and Drug Induced Liver Injury / Q000473:pathology / Q000503:physiopathology*; D005688:Galactosamine / Q000037:antagonists & inhibitors; D005966:Glucuronidase / Q000378:metabolism; D006520:Hepatitis, Animal / Q000473:pathology / Q000503:physiopathology; D007728:Kupffer Cells / Q000502:physiology*; D008070:Lipopolysaccharides / Q000378:metabolism; D008163:Luminescent Measurements; D008247:Lysosomes / Q000201:enzymology; D012157:Mononuclear Phagocyte System / Q000503:physiopathology; D009504:Neutrophils; D010587:Phagocytosis; D011112:Polymyxin B / Q000494:pharmacology; D051381:Rats; D013481:Superoxides / Q000378:metabolism*
chemical_list: D008070:Lipopolysaccharides; D013481:Superoxides; D005688:Galactosamine; D005966:Glucuronidase; D011112:Polymyxin B