Use of silica gel with aqueous eluent for simultaneous high performance liquid chromatographic assay of disopyramide and mono-N-dealkyldisopyramide.
Disopyramide and its pharmacologically active metabolite, mono-N-dealkyldisopyramide, were determined simultaneously in serum by high performance liquid chromatography using a bare (unbonded) silica gel with aqueous eluents. p-Chlorodisopyramide was used as the internal standard. Separations of all compounds, which contain amine moiety in their chemical structures, were easily accomplished with a good peak symmetry on silica. The method is sufficiently precise, sensitive, and specific. Analytical recoveries of all compounds were greater than 96%; CVs for reproducibility were less than 6.5% for disopyramide and mono-N-dealkyldisopyramide; the lower detection limits of both analytes were 0.05 mg/L. Comparison of the present method with a fluorescence polarization immunoassay for disopyramide gives a good correlation (r = 0.992).
D002851:Chromatography, High Pressure Liquid; D004206:Disopyramide / Q000031:analogs & derivatives* / Q000097:blood*; D006801:Humans; D007202:Indicators and Reagents; D007700:Kinetics; D008991:Monitoring, Physiologic; D058428:Silica Gel; D012822:Silicon Dioxide
D007202:Indicators and Reagents; C030438:4-(isopropylamino)-2-(2-pyridyl)-2-phenylbutyramide; D058428:Silica Gel; D012822:Silicon Dioxide; D004206:Disopyramide