[Rapid diagnosis of rotavirus infections by RNA electrophoresis on polyacrylamide gel].
A method of rapid diagnosis of rotavirus infections is described. It is based on the isolation of viral RNA from the feces of patients with viral gastroenteritis and determination of RNA mobility by 6% polyacrylamide gel electrophoresis. It was demonstrated that rotavirus infection could be diagnosed after long-term storage of fecal specimens as well. The method is of interest for epidemiological and genetic studies of human rotavirus diseases.
D004591:Electrophoresis, Polyacrylamide Gel; D005069:Evaluation Studies as Topic; D005243:Feces / Q000382:microbiology; D006801:Humans; D008854:Microscopy, Electron; D012367:RNA, Viral / Q000032:analysis* / Q000302:isolation & purification; D012401:Rotavirus / Q000032:analysis* / Q000302:isolation & purification; D012400:Rotavirus Infections / Q000175:diagnosis* / Q000382:microbiology; D014776:Virus Cultivation
D012367:RNA, Viral
Bystraia diagnostika rotavirusnykh infektsiĭ putem élektroforeza RNK v poliakrilamidnom gele.