Metabolism of leukotriene E4 by rat tissues: formation of N-acetyl leukotriene E4.
Leukotriene E4 was incubated with subcellular fractions from rat liver homogenates. A product identified as 5-hydroxy-6-S-(2-acetamido-3-thiopropionyl)-7,9-trans-11,14- cis-eicosatetraenoic acid (N-acetyl leukotriene E4) was formed. Enzymes catalyzing the reaction were associated with particulate fractions sedimenting between 600 and 8500 g and 20,000 and 105,000 g. Acetyl coenzyme A served as the donor of the acetyl group. N-Acetyl leukotriene E4 was also formed by the 105,000g sediment fractions from kidney, spleen, skin, and lung. The myotropic activity of N-acetyl leukotriene E4 on isolated guinea pig ileum was reduced over 100-fold compared to that of leukotriene D4.
D000107:Acetylation; D000111:Acetylcysteine / Q000378:metabolism; D000818:Animals; D002474:Cell-Free System; D005260:Female; D066298:In Vitro Techniques; D017999:Leukotriene E4 / Q000031:analogs & derivatives; D008099:Liver / Q000378:metabolism; D013058:Mass Spectrometry; D051381:Rats; D011919:Rats, Inbred Strains; D013189:SRS-A / Q000031:analogs & derivatives* / Q000378:metabolism; D014316:Tritium
D013189:SRS-A; D014316:Tritium; D017999:Leukotriene E4; C047630:N-acetylleukotriene E4; D000111:Acetylcysteine