Patterns of polypeptide synthesis in human rotavirus infected cells.
Polypeptide analysis of three strains of human rotavirus (KUN, Wa and MO) were conducted using a hypertonic culture which suppressed host protein synthesis and unmasked rotavirus specific protein synthesis. As a result, eleven human rotavirus specific polypeptides (Vp 1--Vp 11) were detected by pulselabeling infected cells with [14C]-leucine. Among the 11 polypeptides, three polypeptides (Vp 7, Vp 10 and Vp 11) underwent post-translational processing, and two (Vp 7 and Vp 10) were glycosylated. Six polypeptides (Vp 1, 2, 3, 4, 6 and 7) were identified as viral structural proteins. Comparisons of three strains of different serotypes revealed that their polypeptide profiles differed from each other in electrophoretic mobility; in particular, profiles of the glycosylated polypeptide, Vp 7, were distinct among the three strains.
D000818:Animals; D002460:Cell Line; D006801:Humans; D007668:Kidney; D008253:Macaca mulatta; D010452:Peptide Biosynthesis; D011499:Protein Processing, Post-Translational; D012401:Rotavirus / Q000145:classification / Q000378:metabolism*; D014764:Viral Proteins / Q000096:biosynthesis*; D015678:Viral Structural Proteins; D014779:Virus Replication
D014764:Viral Proteins; D015678:Viral Structural Proteins