Interindividual heterogeneity of molecular weight of human brain neutral sphingomyelinase determined by radiation inactivation method.
The molecular weight (Mr) of the membrane-bound neutral sphingomyelinase from human brain was determined using the radiation inactivation procedure. Previous studies on three human brains suggested a Mr of 165 +/- 25 kDa (J. Neurochem. 1985, 45:630-632). We now report that in another human brain the neutral sphingomyelinase had a Mr of 740 +/- 100 kDa; this higher Mr was not accompanied by differences in enzymatic properties nor heat-stability.
D000328:Adult; D000368:Aged; D001921:Brain / Q000201:enzymology* / Q000528:radiation effects / Q000648:ultrastructure; D002462:Cell Membrane / Q000201:enzymology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008297:Male; D008875:Middle Aged; D008970:Molecular Weight; D010727:Phosphoric Diester Hydrolases / Q000032:analysis*; D013108:Sphingomyelin Phosphodiesterase / Q000032:analysis* / Q000528:radiation effects; D013347:Subcellular Fractions / Q000201:enzymology
D010727:Phosphoric Diester Hydrolases; D013108:Sphingomyelin Phosphodiesterase