Classification of Barmah Forest virus as an alphavirus using cytotoxic T cell assays.
Barmah Forest virus, an arbovirus, does not cross-react convincingly with alpha-, flavi- or bunyavirus immune sera. Secondary cytotoxic T cells generated in vitro immune to a number of alphaviruses cross-lyse Barmah Forest virus-infected target cells. Flavivirus (West Nile and Kunjin)- and Bunyamwera virus-immune Tc cells lyse homologous virus-infected target cells, but not alphavirus-infected targets. Using cytotoxic T cell assays Barmah Forest virus can be classified as an alphavirus.
D000524:Alphavirus / Q000145:classification* / Q000254:growth & development / Q000276:immunology; D000818:Animals; D003429:Cross Reactions; D005260:Female; D051379:Mice; D008808:Mice, Inbred CBA; D013045:Species Specificity; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D010948:Viral Plaque Assay