Mechanism of adenosine production by isolated rat heart mitochondria.
Adenosine production by isolated rat heart mitochondria was examined and was observed to be dependent on an active adenine nucleotide transporter and a functional 5'-nucleotidase. It was found that mitochondria do not transport adenosine. These results suggest that mitochondria provide AMP for an extramitochondrial 5'-nucleotidase and this was verified by direct measurement of extramitochondrial levels of AMP and adenosine. A possible role for mitochondria in myocardial adenosine production is discussed.
D015720:5'-Nucleotidase; D000241:Adenosine / Q000096:biosynthesis*; D000244:Adenosine Diphosphate / Q000378:metabolism; D000249:Adenosine Monophosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001692:Biological Transport; D066298:In Vitro Techniques; D008297:Male; D008929:Mitochondria, Heart / Q000378:metabolism*; D009708:Nucleotidases / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D000249:Adenosine Monophosphate; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D009708:Nucleotidases; D015720:5'-Nucleotidase; D000241:Adenosine