title: A DNA binding protein specific for an origin of replication of herpes simplex virus type 1.
abstract: We have identified a protein that binds specifically to an origin of replication (oris) of the herpes simplex virus type 1 genome. The oris binding protein, detectable only in nuclear extracts of infected cells, shows the same time course of appearance as the herpesvirus-induced DNA polymerase and the DNA binding protein ICP8. The partially purified oris binding protein generates a DNase I "footprint" that spans 18- of the 90-base-pair minimal oris sequence. The oris binding protein may, therefore, be analogous to other origin-specific binding proteins that are required for the initiation of viral and chromosomal DNA replication.
mesh_terms: D001483:Base Sequence; D001665:Binding Sites; D004261:DNA Replication; D004268:DNA-Binding Proteins / Q000235:genetics*; D005814:Genes, Viral; D018139:Simplexvirus / Q000235:genetics*; D013997:Time Factors; D014764:Viral Proteins / Q000235:genetics; D014779:Virus Replication
chemical_list: D004268:DNA-Binding Proteins; D014764:Viral Proteins