Characterization of three HLA-DR beta genes isolated from an HLA-DR 3/4 insulin-dependent diabetic patient.
Three HLA-DR beta genes were isolated from a Swedish HLA-DR3/4 insulin-dependent diabetes mellitus (IDDM) patient and characterized by restriction endonuclease mapping and nucleotide sequence analysis. Two out of the three DNA sequences differed from those of published DR beta-chain sequences. A DR beta-gene probe prepared from exon 4 and flanking sequences was used in a Southern blot analysis of blood donors' DNA and DNA from HLA-DR3/4 IDDM patients and HLA-DR-matched healthy control subjects. This probe differentiated HLA-DR3/4 IDDM patients and HLA-DR-matched controls in the Scandinavian population but not in the North American Caucasoid population.
D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D004262:DNA Restriction Enzymes; D003922:Diabetes Mellitus, Type 1 / Q000235:genetics / Q000276:immunology*; D005796:Genes; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II / Q000235:genetics*; D006801:Humans; D011110:Polymorphism, Genetic
D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II; D004262:DNA Restriction Enzymes