Restriction endonuclease activity induced by NC-1A virus infection of a Chlorella-like green alga.
A type II restriction endonuclease, CviBI, was isolated from a eukaryotic, Chlorella-like green alga infected with the dsDNA containing virus NC-1A. The enzyme recognizes the sequence GANTC and cleaves DNA between the G and A. Methylation of deoxyadenosine in the GANTC sequence probably inhibits enzyme activity. In vitro CviBI cleaves host nuclear DNA but not viral DNA. A survey of 18 other viruses which infect the same Chlorella sp. revealed that infection with 5 of these viruses also induced a restriction endonuclease which cleaves DNA into the same size fragments as CviBI.
D001483:Base Sequence; D002708:Chlorella / Q000201:enzymology*; D004247:DNA / Q000378:metabolism; D004248:DNA (Cytosine-5-)-Methyltransferases; D004262:DNA Restriction Enzymes / Q000302:isolation & purification* / Q000378:metabolism; D004267:DNA Viruses / Q000502:physiology*; D004279:DNA, Viral / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific; D004790:Enzyme Induction; D008745:Methylation; D010942:Plant Viruses / Q000502:physiology*
D004279:DNA, Viral; D004247:DNA; D004248:DNA (Cytosine-5-)-Methyltransferases; D004262:DNA Restriction Enzymes; D015252:Deoxyribonucleases, Type II Site-Specific; C032229:GANTC-specific type II deoxyribonucleases