[Adenosine transformation into adenosine-5'-monophosphate by intact Erwinia herbicola cells].
Strain 47/3 was isolated from the natural sources of Erwinia herbicola. The cells of this strain contain nucleoside phosphate transferase responsible for specific phosphorylation of the adenosine 5'-hydroxyl group. With the use of the strain intact cells, conditions for preparation of AMP were determined. The cells were grown in the meat-peptone broth supplemented with yeast extract. p-Nitrophenylphosphate was used as a donor of phosphate groups. It was shown that the concentration of the reaction substrates, their ratio and PH of the reaction mixture were factors defining the cell activity. Under the optimal conditions (0.2 M acetate buffer, pH 4.5; concentrations of p-nitrophenylphosphate and adenosine 96 and 12 mg/ml respectively; concentration of the cells 1 per cent) the cells phosphorylated adenosine with a yield of 74 mol %. This indicates that the method may be used for production of AMP in preparative amounts.
D000241:Adenosine / Q000378:metabolism*; D000249:Adenosine Monophosphate / Q000096:biosynthesis*; D003470:Culture Media / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004885:Erwinia / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D009596:Nitrophenols / Q000378:metabolism; D009943:Organophosphorus Compounds / Q000378:metabolism; D010766:Phosphorylation; D010770:Phosphotransferases / Q000378:metabolism; D013696:Temperature
D003470:Culture Media; D009596:Nitrophenols; D009943:Organophosphorus Compounds; C008644:nitrophenylphosphate; D000249:Adenosine Monophosphate; D010770:Phosphotransferases; C021892:nucleoside phosphotransferase; D000241:Adenosine
Transformatsiia adenozina v adenozin-5'-monofosfat intaktnymi kletkami Erwinia herbicola.