title: Characterization of murine peritoneal macrophage receptors for fibrin(ogen) degradation products.
abstract: The binding of human fibrinogen degradation fragments D1, E, X, and Y, as well as fibrin fragment D1 dimer, to mouse peritoneal macrophages was examined. A Scatchard plot of fragment D1 binding was biphasic, suggesting two classes of receptors. Fragments D1, D1 dimer, X, and Y in low concentrations bound to macrophages with high affinity (Kd = 23 to 73 X 10(-11) mol/L). Fragment E bound specifically but at a much lower level than the other fragments. Fragment D1 was able to compete for the binding of radiolabeled fragments X and Y but not radiolabeled fragment E. These studies indicate that fragments D and E are recognized by separate receptor systems but that all of the fibrinogen degradation products that contain the D domain are recognized by the same receptor system.
mesh_terms: D000818:Animals; D001202:Ascitic Fluid; D001667:Binding, Competitive; D002851:Chromatography, High Pressure Liquid; D005338:Fibrin Fibrinogen Degradation Products / Q000378:metabolism*; D007700:Kinetics; D046911:Macromolecular Substances; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D011956:Receptors, Cell Surface / Q000378:metabolism*; D018000:Receptors, Peptide
chemical_list: D005338:Fibrin Fibrinogen Degradation Products; D046911:Macromolecular Substances; D011956:Receptors, Cell Surface; D018000:Receptors, Peptide; C048650:fibrin-fibrinogen degradation products receptor; C015214:fibrinogen D fragment; C018543:fibrinogen fragment E; C030568:fibrinogen fragment X; C036129:fibrinogen fragment Y