title: Streptococcus faecalis R plasmid pJH1 contains a pAM alpha 1 delta 1-like replicon.
abstract: Streptococcus faecalis R plasmid pJH1 did not transform competent strains of Streptococcus sanguis. A hybrid plasmid, pDL310, consisting of virtually all of the S. faecalis hemolysin-bacteriocin plasmid pJH2 and a segment of pJH1 DNA that included the tetracycline resistance determinant, yielded tetracycline-resistant transformants at a frequency of less than 10(-8) transformants per CFU, when it was added to a competent culture of S. sanguis Wicky. Four of the transformants contained a 4.7-kilobase plasmid (pDL316) that transformed strain Wicky at a frequency of 8.6 X 10(-8). Restriction endonuclease digests, agarose gel electrophoresis, and Southern blot hybridizations indicated that pDL316 consisted entirely of pJH1-derived DNA. Additional restriction studies, Southern blot hybridizations, and heteroduplex analyses indicated that pDL316 was very closely related to 4.6-kilobase tetracycline resistance plasmid pAM alpha 1 delta 1, a derivative of 9.0-kilobase S. faecalis plasmid pAM alpha 1.
mesh_terms: D001430:Bacteriocins / Q000235:genetics; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000032:analysis; D004352:Drug Resistance, Microbial; D013293:Enterococcus faecalis / Q000187:drug effects / Q000235:genetics*; D006460:Hemolysin Proteins / Q000235:genetics; D009692:Nucleic Acid Heteroduplexes; D009693:Nucleic Acid Hybridization; D011815:R Factors; D012093:Replicon; D012689:Sequence Homology, Nucleic Acid; D013752:Tetracycline / Q000494:pharmacology; D014169:Transformation, Bacterial
chemical_list: D001430:Bacteriocins; D004269:DNA, Bacterial; D006460:Hemolysin Proteins; D009692:Nucleic Acid Heteroduplexes; D004262:DNA Restriction Enzymes; D013752:Tetracycline