Separation of corticosteroids by high-performance liquid chromatography on dynamically modified silica.
High-performance liquid chromatographic methods have been elaborated for the separation of 12 different corticosteroids by reversed-phase chromatography on bare silica, dynamically modified by cetyltrimethylammonium bromide added to the eluent. The selectivity of the systems towards a mixture of the 12 corticosteroids and towards a mixture of hydrocortisone and its related impurities were investigated using different brands of silica. The variations in selectivity were found to be substantially smaller than those of chromatographic systems based on chemically bonded ODS-silicas from the same sources.
D000305:Adrenal Cortex Hormones / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D006854:Hydrocortisone / Q000302:isolation & purification; D012822:Silicon Dioxide; D013056:Spectrophotometry, Ultraviolet
D000305:Adrenal Cortex Hormones; D012822:Silicon Dioxide; D006854:Hydrocortisone