title: Humoral hypercalcaemia of malignancy: report of two further patients with biochemical studies on tumour extracts.
abstract: Tumour extracts from two patients with humoral hypercalcaemia of malignancy contained material which stimulated adenylate cyclase in chick renal membranes and in rat osteosarcoma cells. Adenylate cyclase-stimulating activity in each system was inhibited by a specific parathyroid hormone (PTH) antagonist. Studies in two HPLC systems suggested that the adenylate cyclase-stimulating factors extracted from these tumours differed from each other and from synthetic human parathyroid hormone 1-34. The presence of similar PTH-like adenylate cyclase stimulating material(s) in oncogenic osteomalacia suggests that adenylate cyclase stimulating factor(s) may not be the direct or the sole cause of hypercalcaemia.
mesh_terms: D000262:Adenylyl Cyclases / Q000096:biosynthesis; D000328:Adult; D000368:Aged; D002292:Carcinoma, Renal Cell / Q000032:analysis / Q000150:complications*; D002851:Chromatography, High Pressure Liquid; D000242:Cyclic AMP / Q000096:biosynthesis; D005260:Female; D006801:Humans; D006934:Hypercalcemia / Q000209:etiology* / Q000378:metabolism*; D007668:Kidney / Q000378:metabolism; D007680:Kidney Neoplasms / Q000032:analysis / Q000150:complications*; D007866:Leg; D008297:Male; D008545:Melanoma / Q000032:analysis / Q000150:complications*; D012516:Osteosarcoma / Q000378:metabolism; D010281:Parathyroid Hormone / Q000032:analysis* / Q000494:pharmacology
chemical_list: D010281:Parathyroid Hormone; D000242:Cyclic AMP; D000262:Adenylyl Cyclases