Effect of epidermal growth factor on the membrane potential of cultured porcine thyroid cells.
Cultured porcine thyroid cells maintained in media containing TSH exhibited a membrane potential of -50 mV, and hyperpolarized by about 10 mV within 1 h of the addition of epidermal growth factor (EGF; 10 ng/ml). Follicle cells had depolarized to -45 mV after 4 h of exposure to EGF. Cells maintained in dibutyryl cyclic AMP (dbcAMP) did not alter their membrane potential when exposed to EGF for up to 4 h. Cultures washed to remove the TSH or dbcAMP hyperpolarized to -75 mV within 30 min, and a reversible depolarization to -60 mV was observed on addition of EGF. It was concluded that EGF acts as a physiological antagonist of TSH and also exerts a separate depolarizing influence on cultured thyroid cells.
D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D002478:Cells, Cultured; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D008564:Membrane Potentials / Q000187:drug effects; D013552:Swine; D013961:Thyroid Gland / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D013972:Thyrotropin / Q000494:pharmacology
D004815:Epidermal Growth Factor; D003994:Bucladesine; D013972:Thyrotropin