title: An electron spin resonance study of free radical intermediates in the oxidation of indole acetic acid by horseradish peroxidase.
abstract: The oxidation of indole-3-acetic acid by horseradish peroxidase was studied using the spin traps t-nitrosobutane and 5,5-dimethyl-1-pyrroline N-oxide to trap free radical intermediates. The major free radical metabolite of indole acetic acid was unambiguously determined by the use of indole-3-[2,2-2H2]acetic acid to be the skatole carbon-centered free radical. In the presence of oxygen, superoxide was also trapped.
mesh_terms: D004578:Electron Spin Resonance Spectroscopy; D005609:Free Radicals; D006735:Horseradish Peroxidase / Q000378:metabolism*; D007210:Indoleacetic Acids / Q000378:metabolism*; D010084:Oxidation-Reduction; D010544:Peroxidases / Q000378:metabolism*; D013481:Superoxides
chemical_list: D005609:Free Radicals; D007210:Indoleacetic Acids; D013481:Superoxides; C030737:indoleacetic acid; D006735:Horseradish Peroxidase; D010544:Peroxidases