Inhibition of bacterial DNA-dependent RNA polymerases and restriction endonuclease by UV-absorbing components from propolis.
Several UV-absorbing substances inhibiting the DNA-dependent RNA polymerases of Escherichia coli and Streptomyces aureofaciens, as well as the restriction endonuclease Eco RI have been isolated from the water-soluble extract of Propolis by two-dimensional paper chromatography. The inhibition of bacterial RNA-polymerases by the components of Propolis was probably due to the loss of their ability to bind to DNA. The general characteristic of the UV-absorbing component of Propolis with the most pronounced inhibitory effect upon transcription in vitro is described.
D002854:Chromatography, Paper; D004262:DNA Restriction Enzymes / Q000037:antagonists & inhibitors*; D004279:DNA, Viral / Q000032:analysis; D012321:DNA-Directed RNA Polymerases / Q000037:antagonists & inhibitors*; D003850:Deoxyribonuclease I / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology; D010179:Pancreas / Q000201:enzymology; D011429:Propolis / Q000032:analysis / Q000494:pharmacology*; D012116:Resins, Plant / Q000494:pharmacology*; D013056:Spectrophotometry, Ultraviolet; D014158:Transcription, Genetic / Q000187:drug effects
D004279:DNA, Viral; D012116:Resins, Plant; D011429:Propolis; D012321:DNA-Directed RNA Polymerases; D004262:DNA Restriction Enzymes; D003850:Deoxyribonuclease I