Effect of reductones on cyclic 3', 5'-adenosine monophosphate phosphodiesterase.
The effect of some reductones such as ascorbic acid (AsA), triose reductone (TR), epinephrine (Ep) and their derivatives on cyclic 3', 5'-adenosine monophosphate phosphodiesterase (cAMP PDE) was studied in the presence or absence of Cu2+. AsA, TR, Ep and the reductones related to them inhibited cAMP PDE activity. Among the reductones, TR showed the highest inhibition. AsA, 5-methyl-3,4-dihydroxytetrone, pyrocatechol, p-hydroxyquinone and resorcinol had a relatively high inhibiting activity. The type of inhibition of AsA, TR and Ep was uncompetitive, competitive and noncompetitive, respectively. Cu2+ enhanced the inhibitory action of the reductones markedly and altered the type of inhibition of the reductones.
D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000378:metabolism*; D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology*; D002413:Cations, Divalent; D002417:Cattle; D002729:Chloromercuribenzoates / Q000494:pharmacology; D003300:Copper / Q000494:pharmacology; D004837:Epinephrine / Q000494:pharmacology*; D007700:Kinetics; D008314:Malonates; D008315:Malondialdehyde / Q000031:analogs & derivatives* / Q000494:pharmacology; D009206:Myocardium / Q000201:enzymology; D013329:Structure-Activity Relationship; D020245:p-Chloromercuribenzoic Acid
D002413:Cations, Divalent; D002729:Chloromercuribenzoates; D008314:Malonates; C010778:reductone; D008315:Malondialdehyde; D020245:p-Chloromercuribenzoic Acid; D003300:Copper; D015105:3',5'-Cyclic-AMP Phosphodiesterases; D001205:Ascorbic Acid; D004837:Epinephrine