Expression of the Epstein-Barr virus major membrane proteins in Chinese hamster ovary cells.
The gene of the major membrane antigen (gp250/350) of the Epstein-Barr virus (EBV) was isolated and inserted under the control of the SV40 early promoter into a eukaryotic expression vector, which allows selection for dhfr+ phenotype. Following transfection with this vector, Chinese hamster ovary cells express on their surfaces proteins immunologically similar to the major EBV membrane antigen. The transcript encoding gp250/350 is processed by partial splicing similarly but more efficiency than in B95-8 cells from which the DNA originates.
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002472:Cell Transformation, Viral; D006224:Cricetinae; D003412:Cricetulus; D004262:DNA Restriction Enzymes; D005260:Female; D005796:Genes; D005814:Genes, Viral; D005822:Genetic Vectors; D004854:Herpesvirus 4, Human / Q000235:genetics*; D010053:Ovary; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic; D014763:Viral Matrix Proteins; D014764:Viral Proteins / Q000235:genetics*
D014763:Viral Matrix Proteins; D014764:Viral Proteins; D004262:DNA Restriction Enzymes