title: Isolation of ntrA-like mutants of Azotobacter vinelandii.
abstract: A number of chlorate-resistant mutants of Azotobacter vinelandii affected in a general control of nitrogen metabolism were isolated. These mutants could not utilize dinitrogen, nitrate, or nitrite as a nitrogen source. The reason for this inability is that they were simultaneously deficient in nitrogenase and nitrate and nitrite reductase activities. They were complemented by a cosmid carrying a DNA fragment of A. vinelandii able to complement ntrA mutants of Escherichia coli, so they seemed to be ntrA-like mutants.
mesh_terms: D001395:Azotobacter / Q000201:enzymology / Q000235:genetics*; D001426:Bacterial Proteins / Q000494:pharmacology; D002704:Chlorates / Q000494:pharmacology; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000235:genetics; D005816:Genetic Complementation Test; D008274:Magnesium / Q000378:metabolism; D008345:Manganese / Q000378:metabolism; D009154:Mutation; D009565:Nitrate Reductases / Q000235:genetics; D009572:Nitrite Reductases / Q000235:genetics; D009584:Nitrogen / Q000378:metabolism; D009586:Nitrogen Fixation; D009591:Nitrogenase / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D002704:Chlorates; D008345:Manganese; D009591:Nitrogenase; D009565:Nitrate Reductases; D009572:Nitrite Reductases; D008274:Magnesium; D009584:Nitrogen