title: The reaction of ferrous EDTA with hydrogen peroxide: evidence against hydroxyl radical formation.
abstract: Radiolytically generated hydroxyl radicals degrade cytochrome c. In contrast, ferrocytochrome c is oxidized in the Fe-EDTA-H2O2 system, without loss of any cytochrome c. It is concluded that in the latter system no hydroxyl radicals are formed and that oxidation takes place via a FeO(II)EDTA or Fe(II)-H2O2-EDTA complex.
mesh_terms: D003574:Cytochrome c Group / Q000378:metabolism; D004492:Edetic Acid / Q000378:metabolism*; D005290:Ferric Compounds / Q000378:metabolism; D005609:Free Radicals; D006861:Hydrogen Peroxide / Q000378:metabolism*; D006878:Hydroxides / Q000378:metabolism; D017665:Hydroxyl Radical; D066298:In Vitro Techniques; D007700:Kinetics
chemical_list: D003574:Cytochrome c Group; D005290:Ferric Compounds; D005609:Free Radicals; D006878:Hydroxides; D017665:Hydroxyl Radical; D004492:Edetic Acid; D006861:Hydrogen Peroxide; C019179:Fe(III)-EDTA