Potentiation of 5-lipoxygenase metabolism by human neutrophils using a combination of activators.
Platelet-derived 12-hydroperoxy-5,8,10,14-icosatetraenoic acid (12-HPETE) cannot increase the synthesis of leukotriene B4 metabolites by human neutrophils activated by 1-0-hexadecyl-2-acetyl-sn-glycero-3-phosphocholine (Paf Acether) at 10(-9) and 10(-8) M in contrast to 1 microM arachidonic acid. The combination of the 3 inducers at various concentrations resulted in a potentiation of the formation of leukotriene B4 metabolites suggesting that multiregulatory steps play a key role to govern the 5-lipoxygenase activity. These results suggest that the use of agents that can act synergistically to produce leukotrienes may be worthwhile investigating to understand some aspects of the leukotriene biosynthesis.
D001094:Arachidonate 5-Lipoxygenase / Q000378:metabolism*; D001091:Arachidonate Lipoxygenases / Q000378:metabolism*; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000494:pharmacology*; D004357:Drug Synergism; D006801:Humans; D007975:Leukotriene B4 / Q000378:metabolism; D015289:Leukotrienes; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D010972:Platelet Activating Factor / Q000502:physiology; D013268:Stimulation, Chemical
D001095:Arachidonic Acids; D015289:Leukotrienes; D010972:Platelet Activating Factor; D007975:Leukotriene B4; D016718:Arachidonic Acid; C021201:12-HPETE; D001091:Arachidonate Lipoxygenases; D001094:Arachidonate 5-Lipoxygenase