Molecular cloning and sequence analysis of the human parainfluenza 3 virus mRNA encoding the P and C proteins.
The sequence of the mRNA encoding the phosphoprotein (P protein) of the human parainfluenza virus 3 (PF3) was determined by molecular cloning. In other Parmyxoviridae the P protein mRNA is functionally bicistronic and encodes an additional smaller nonstructural protein termed C. In this report three open reading frames (ORF) are described. These consist of a single long ORF encoding the P protein, and two shorter ORFs encoding the structural Vp18 protein (analogous to the Sendai C protein) and a putative polypeptide termed D protein. The encoded phosphoprotein consists of 603 amino acids and has a predicted molecular weight of 67,683. The C protein consists of 199 amino acids and has a predicted molecular weight of 23,288. The D protein consists of 140 amino acids and has a predicted molecular weight of 16,270. Although the D protein has not yet been demonstrated in vivo its synthesis could be demonstrated in vitro using a rabbit reticulocyte lysate system. Thus it appears that unlike the other paramyxoviruses, the PF3 P protein mRNA may be functionally tricistronic.
D000595:Amino Acid Sequence; D001483:Base Sequence; D005075:Biological Evolution; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D005814:Genes, Viral; D008970:Molecular Weight; D010222:Parainfluenza Virus 1, Human / Q000235:genetics; D010224:Parainfluenza Virus 3, Human / Q000235:genetics*; D010750:Phosphoproteins / Q000235:genetics*; D014176:Protein Biosynthesis; D011487:Protein Conformation; D012333:RNA, Messenger / Q000235:genetics; D018108:Respirovirus / Q000235:genetics*; D012995:Solubility; D014764:Viral Proteins / Q000235:genetics*
D010750:Phosphoproteins; D012333:RNA, Messenger; D014764:Viral Proteins; D004247:DNA