title: The protein kinase C inhibitors H-7 and H-9 fail to inhibit human neutrophil activation.
abstract: The protein kinase C inhibitors 1-(5-isoquinolinylsulfonyl)-2-methyl-piperazine (H-7) and N-(2-aminoethyl)-5-isoquinolinesulfonamide (H-9) were examined for their ability to inhibit human neutrophil activation. At concentrations up to 100 micromolar, these compounds failed to inhibit either respiratory burst or the secretory response of neutrophils stimulated with particulate (serum-opsonized zymosan) or soluble (A23187, FMLP, PMA) stimuli. In contrast, the calmodulin antagonist N-(6-aminohexyl)-5-chloro-1-naphthalene-sulfonamide (W-7) inhibited both oxygen radical generation and lysosomal enzyme release in response to the same stimuli. These results suggest that calmodulin-dependent enzymes, rather than protein kinase C, may be essential for neutrophil activation.
mesh_terms: D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D006801:Humans; D007546:Isoquinolines / Q000494:pharmacology*; D008247:Lysosomes / Q000201:enzymology; D009113:Muramidase / Q000378:metabolism; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D010101:Oxygen Consumption / Q000187:drug effects; D009195:Peroxidase / Q000378:metabolism; D010879:Piperazines / Q000494:pharmacology*; D011493:Protein Kinase C / Q000037:antagonists & inhibitors*; D012636:Secretory Rate / Q000187:drug effects; D013449:Sulfonamides / Q000494:pharmacology; D013481:Superoxides / Q000378:metabolism
chemical_list: D007546:Isoquinolines; D010879:Piperazines; D013449:Sulfonamides; D013481:Superoxides; C017967:W 7; C034700:N-(6-aminohexyl)-1-naphthalenesulfonamide; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D009195:Peroxidase; D011493:Protein Kinase C; D009113:Muramidase