Angiotensin-converting enzyme (ACE) measurement in human serum using radioinhibitor ligand binding.
MK351A, a tyrosyl analogue of enalaprilic acid (MK422) is a potent inhibitor of angiotensin-converting enzyme (ACE). MK351A was radioiodinated with 125I and used to develop a radio inhibitor binding assay for human serum ACE. 125I MK351A associated rapidly and reversibly with human serum ACE (T 1/2 = 1/2 h). Bound 125I MK351A was displaced by an excess of cold MK351A, to give non-specific binding of less than 1%. Scatchard analysis of binding was linear (r = -0.99, n = 6, p less than 0.001), indicating a single class of binding site. ACE was estimated in serum from normal patients and patients with sarcoid by the radio inhibitor binding assay and by enzyme kinetic assay using Hip-His-Leu as substrate. The two methods for ACE estimation correlated closely (r = 0.87, n = 82, p less than 0.001). The radio inhibitor binding assay for human serum ACE is a simple, sensitive and specific assay which utilizes novel assay methodology.
D000806:Angiotensin-Converting Enzyme Inhibitors; D004151:Dipeptides / Q000378:metabolism; D006801:Humans; D007457:Iodine Radioisotopes; D007700:Kinetics; D008722:Methods; D007703:Peptidyl-Dipeptidase A / Q000097:blood*; D012507:Sarcoidosis / Q000201:enzymology
D000806:Angiotensin-Converting Enzyme Inhibitors; D004151:Dipeptides; D007457:Iodine Radioisotopes; C042037:L-proline, N2-((1S)-1-carboxy-3-phenylpropyl)-N6-((4-hydroxyphenyl)iminomethyl)-L-lysyl-; D007703:Peptidyl-Dipeptidase A