Polymorphisms within the HLA-DR4 haplotypes. Various DQ subtypes detected with monoclonal antibodies.
Polymorphisms among HLA class II molecules expressed by cells with different HLA-DR4 haplotypes were analysed biochemically (isoelectrofocussing and 2D gels), cellularly (HLA-Dw) and serologically (monoclonal antibodies). The results confirm the correlation which exists between HLA-D specificity and DR beta chain isoelectric point polymorphism. Furthermore, a biochemical polymorphism was observed among DQw3 molecules. No correlation was found with HLA-Dw types. On the other hand a correlation was found between DQ-polymorphism and TA10 and 2B3 specificities defined by monoclonal antibodies. The comparison of different methods defining polymorphisms of HLA class II molecules will be discussed.
D000483:Alleles; D000911:Antibodies, Monoclonal; D001402:B-Lymphocytes / Q000276:immunology; D002460:Cell Line; D002472:Cell Transformation, Viral; D004591:Electrophoresis, Polyacrylamide Gel; D005802:Genes, MHC Class II; D008040:Genetic Linkage; D015804:HLA-DR4 Antigen; D004854:Herpesvirus 4, Human / Q000235:genetics; D000949:Histocompatibility Antigens Class II / Q000032:analysis*; D006801:Humans; D011110:Polymorphism, Genetic
D000911:Antibodies, Monoclonal; D015804:HLA-DR4 Antigen; D000949:Histocompatibility Antigens Class II