A selective screen for transposable element mobilization in Drosophila melanogaster.
A selective system is described that provides a simple and sensitive assay for the detection and analysis of induced mobile element transpositions in Drosophila melanogaster. The system will detect a single event in samples greater than 10(6) and thus provides a eucaryotic assay system for monitoring the induction of transposition by a variety of agents including, but not limited to, chemical carcinogens and toxins, ionizing radiation, and various environmental pollutants. The experimental system focuses on an X-linked rosy+ transposon and a conditional lethal system that permits the detection of a single transposition event in very large samples. The results of a pilot experiment utilizing this system are presented.
D000818:Animals; D004262:DNA Restriction Enzymes; D004251:DNA Transposable Elements; D004331:Drosophila melanogaster / Q000235:genetics*; D009152:Mutagenicity Tests; D014968:Xanthine Dehydrogenase / Q000235:genetics
D004251:DNA Transposable Elements; D014968:Xanthine Dehydrogenase; D004262:DNA Restriction Enzymes