Moloney murine sarcoma virus encoded p37mos expressed in yeast has protein kinase activity.
We describe the expression of the Moloney murine sarcoma virus env-mos protein (p37mos) in yeast under the control of the yeast GAL1 promoter. Consistent with our previous results concerning the p37mos protein kinase made in virus infected mouse cells, p37mos produced in yeast possesses autophosphorylation activity in an immune complex kinase assay using anti-mos (37-35) serum.
D005786:Gene Expression Regulation; D008980:Moloney murine sarcoma virus / Q000378:metabolism*; D010766:Phosphorylation; D011401:Promoter Regions, Genetic; D011494:Protein Kinases / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D009053:Sarcoma Viruses, Murine / Q000378:metabolism*; D014764:Viral Proteins / Q000378:metabolism*
D014764:Viral Proteins; D011494:Protein Kinases