High proliferative activity of Reed Sternberg associated antigen Ki-1 positive cells in normal lymphoid tissue.
An improved immunoenzymatic double labelling method was developed, which simultaneously shows the Ki-1 membrane antigen and the nuclear proliferation associated antigen, defined by monoclonal antibody Ki-67. This new approach permits in situ discrimination of cells that are proliferating or not with a particular membrane antigen. Most Ki-1 positive cells in normal lymphoid tissue also express the Ki-67 nuclear antigen and thus appear to be proliferating. As proliferating cells are more susceptible to malignant transformation than quiescent cells, it is possible that the small number of normal Ki-1 positive cells might, none the less, cause a large proportion of all lymphomas in man.
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm / Q000276:immunology*; D034961:Antigens, Nuclear; D000954:Antigens, Surface / Q000032:analysis*; D006689:Hodgkin Disease / Q000276:immunology*; D006801:Humans; D007124:Immunoenzyme Techniques; D017730:Ki-1 Antigen; D008938:Mitosis; D009698:Nucleoproteins / Q000032:analysis; D014066:Palatine Tonsil / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D034961:Antigens, Nuclear; D000954:Antigens, Surface; D017730:Ki-1 Antigen; D009698:Nucleoproteins