Amplification and overexpression of the EGF receptor gene in primary human glioblastomas.
The expression of epidermal growth factor (EGF) receptor in brain tumours of glial origin was studied at the protein, mRNA and genomic levels. Four out of 10 glioblastomas that overexpress EGF receptor also have gene amplification. The amplified genes appear to be rearranged, generating an aberrant mRNA in at least one of these tumours. Such receptor defects may be relevant to tumorigenesis of human glioblastomas.
D001932:Brain Neoplasms / Q000235:genetics*; D002460:Cell Line; D004247:DNA; D004591:Electrophoresis, Polyacrylamide Gel; D004815:Epidermal Growth Factor; D066246:ErbB Receptors; D005784:Gene Amplification; D005786:Gene Expression Regulation; D005910:Glioma / Q000235:genetics*; D006801:Humans; D009693:Nucleic Acid Hybridization; D009857:Oncogenes; D012333:RNA, Messenger; D011956:Receptors, Cell Surface / Q000235:genetics*
D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D004815:Epidermal Growth Factor; D004247:DNA; D066246:ErbB Receptors