Radiotoxicity of 11C-methionine measured by the accumulation of DNA strand breaks in mammalian cells.
The radiotoxic effects of L-[methyl-11C] methionine were estimated by measuring the accumulation of DNA strand breaks. CHO, Chinese-hamster cells, were incubated in 11C-methionine-containing medium for 60 min at 37 degrees C. The number of unrepaired DNA strand breaks was then examined by the DNA-unwinding method. For comparison, cells were also externally irradiated under similar conditions with gamma radiation (137Cs) or positrons (11C). The relative biological effectiveness of 11C-methionine was estimated to be about 1.
D000818:Animals; D001345:Autoradiography; D002250:Carbon Radioisotopes; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D004265:DNA Helicases / Q000528:radiation effects*; D005260:Female; D008715:Methionine / Q000633:toxicity*; D010053:Ovary / Q000187:drug effects* / Q000528:radiation effects; D013936:Thymidine / Q000378:metabolism
D002250:Carbon Radioisotopes; D008715:Methionine; D004265:DNA Helicases; D013936:Thymidine