Rapid dot-immunobinding assay on nitrocellulose for viral antibodies.
A procedure is described for the routine laboratory diagnosis of viral serum antibodies. Antigens are dotted on nitrocellulose strips or sheets, and sera are applied on absorbent paper strips. Antigen-antibody complexes are detected with enzyme-conjugated antiglobulin and development of a colored, insoluble substrate product. The test allows processing of multiple sera in one 3- to 5-h operation and is equal to or more sensitive than serum neutralization, hemagglutination inhibition, and fluorescent antibody assays. Highly infectious viruses inactivated with a psoralen derivative and long-wavelength UV light irradiation can be used as antigens, allowing the study of human pathogens. Although the test detects cross-reacting, group-specific herpesvirus antigens, the intensity of the antibody reaction is greatest with type-specific antigens. Preliminary data suggest that the technique will be useful for the rapid typing of viruses from clinical specimens.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D002522:Chlorocebus aethiops / Q000276:immunology; D003101:Collodion; D003429:Cross Reactions; D003587:Cytomegalovirus / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006385:Hemagglutination Inhibition Tests; D006564:Herpesviridae / Q000276:immunology*; D006801:Humans; D008253:Macaca mulatta / Q000276:immunology; D008459:Measles virus / Q000276:immunology*; D009500:Neutralization Tests; D002679:Pan troglodytes / Q000276:immunology; D012401:Rotavirus / Q000276:immunology*; D012453:Saimiri / Q000276:immunology; D018139:Simplexvirus / Q000276:immunology
D000914:Antibodies, Viral; D003101:Collodion