[Experiments to culture the bovine pestivirus in heterologous cell cultures].
Attempts were made to adapt a strain of the bovine pestivirus to cell cultures of swine kidney in a series of 15 passages. The adaptation of the virus was followed up through the production of a cytopathic effect, through comparative investigations, and by means of cytologic preparations. It was found that the cytopathic effect induced and the titer of the virus grew with increasing the number of the serial passages. Immunofluorescence and cytologic data showed the progressive development of the virus in cell cultures of swine kidney. It was also established that the time of incubation of the infected cultures to produce an overall cytopathic effect was shorter.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D003588:Cytopathogenic Effect, Viral; D001908:Diarrhea Viruses, Bovine Viral / Q000254:growth & development* / Q000276:immunology; D005455:Fluorescent Antibody Technique; D007668:Kidney; D010576:Pestivirus / Q000254:growth & development*; D013552:Swine; D013997:Time Factors; D014776:Virus Cultivation / Q000379:methods
D000914:Antibodies, Viral
Opiti za kultivirane na govezhdiia pestivirus v kheterolozhni kletŭchni kulturi.