Studies on the stimulation of the bacterial, collagenolytic enzyme clostridiopeptidase A by cobalt (II) ions.
Co(II) ions increase the Vmax of clostridiopeptidase A, producing a maximum stimulation of overall enzymic activity of 120%. Co(II) does not displace Zn(II) from the active site, nor Ca(II) from its binding site on the enzyme. There appears to be an additional transition metal-binding site on clostridiopeptidase A, accepting Zn(II), which is inhibitory (Ki = 550 microM), or Co(II), which is stimulatory (Kact = 200 microM).
D003035:Cobalt / Q000494:pharmacology*; D007700:Kinetics; D003012:Microbial Collagenase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D013268:Stimulation, Chemical; D015032:Zinc / Q000494:pharmacology
D003035:Cobalt; D003012:Microbial Collagenase; D015032:Zinc