Analysis of the ruv locus of Escherichia coli K-12 and identification of the gene product.
The ruv gene of Escherichia coli, which is associated with inducible mechanisms of DNA repair and recombination, has been cloned into the low-copy plasmid vector pHSG415. The recombinant plasmid pPVA101 fully complements the DNA repair-deficient phenotype of ruv mutants. Restriction endonuclease analysis of this plasmid revealed a 10.6-kilobase (kb) HindIII DNA insert which contained a 7.7-kb PstI fragment identified as being from the chromosomal ruv region. Deletion analysis and Tn1000 insertional inactivation of ruv function located the ruv coding region to a 2.2-kb section of the cloned DNA fragment. A comparison of the proteins encoded by ruv wild-type and mutant plasmids identified the gene product as a protein of molecular weight 41,000.
D001426:Bacterial Proteins / Q000032:analysis*; D002872:Chromosome Deletion; D002874:Chromosome Mapping; D004260:DNA Repair; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics*; D009154:Mutation; D014466:Ultraviolet Rays
D001426:Bacterial Proteins; D004251:DNA Transposable Elements