Analysis of integrated proviral DNA sequences with an octadecanucleotide probe designed for specific identification of spleen focus-forming virus in the mouse genome.
The Friend spleen focus-forming virus (SFFV) is an envelope gene recombinant between the ecotropic Friend murine leukemia virus and the endogenous xenotropic mink cell focus-forming retroviral sequences. We synthesized an octadecanucleotide complementary to the 3' end of the SFFV env gene designed for discriminating the SFFV proviruses from the xenotropic mink cell focus-forming virus and ecotropic exogenous or endogenous viral sequences. Under appropriate hybridization conditions this probe allowed the identification, in addition to few endogenous DNA fragments, of multiple SFFV proviruses integrated in the genome of Friend malignant cells. Therefore this probe should be of interest in further characterizing the SFFV integration sites and possibly the SFFV ancestor gene.
D000818:Animals; D001483:Base Sequence; D004273:DNA, Neoplasm / Q000032:analysis; D004279:DNA, Viral / Q000032:analysis*; D005622:Friend murine leukemia virus / Q000235:genetics; D005819:Genetic Markers; D009052:Leukemia Virus, Murine / Q000302:isolation & purification*; D007942:Leukemia, Experimental / Q000235:genetics / Q000382:microbiology*; D051379:Mice / Q000235:genetics / Q000382:microbiology; D008908:Mink Cell Focus-Inducing Viruses / Q000235:genetics; D009693:Nucleic Acid Hybridization; D011995:Recombination, Genetic; D013155:Spleen Focus-Forming Viruses / Q000235:genetics / Q000302:isolation & purification*; D014759:Viral Envelope Proteins / Q000235:genetics
D004273:DNA, Neoplasm; D004279:DNA, Viral; D005819:Genetic Markers; D014759:Viral Envelope Proteins