title: Pertussis toxin does not prevent alpha adrenergic stimulated breakdown of phosphoinositides or respiration in brown adipocytes.
abstract: The role of Ni in mediation of alpha adrenergic stimulated respiration and breakdown of phosphatidylinositol 4,5-P2 in brown adipocytes was examined using pertussis toxin. Phenylephrine stimulation of respiration and breakdown of PtdIns-4,5-P2 was still present in adipocytes harvested from hamsters treated with pertussis toxin although toxin modification of Ni appeared complete as judged from the absence of incorporation of [32P] from [32P]-NAD into a 41 KD protein in membranes. These data suggest that alpha-1 receptors on brown adipocytes are not coupled to inositide hydrolysis through Ni.
mesh_terms: D015762:2-Chloroadenosine; D000241:Adenosine / Q000031:analogs & derivatives / Q000494:pharmacology; D002001:Adipose Tissue, Brown / Q000378:metabolism*; D000818:Animals; D006224:Cricetinae; D019204:GTP-Binding Proteins / Q000502:physiology*; D007545:Isoproterenol / Q000494:pharmacology; D008297:Male; D008647:Mesocricetus; D010101:Oxygen Consumption / Q000187:drug effects*; D037342:Pertussis Toxin; D010656:Phenylephrine / Q000494:pharmacology; D010716:Phosphatidylinositols / Q000378:metabolism*; D011433:Propranolol / Q000494:pharmacology; D011942:Receptors, Adrenergic, alpha / Q000502:physiology*; D010566:Virulence Factors, Bordetella / Q000494:pharmacology*
chemical_list: D010716:Phosphatidylinositols; D011942:Receptors, Adrenergic, alpha; D010566:Virulence Factors, Bordetella; D015762:2-Chloroadenosine; D010656:Phenylephrine; D011433:Propranolol; D037342:Pertussis Toxin; D019204:GTP-Binding Proteins; D000241:Adenosine; D007545:Isoproterenol