Effects of chronic ouabain treatment on [3H]ouabain binding sites and electrogenic component of membrane potential in cultured rat myotubes.
The effects of incubation of cultured rat skeletal myotubes in ouabain were studied on the number of [3H]ouabain binding sites and electrogenic component of membrane potential. Ouabain treatment for 2-6 days increased the number of binding sites, resting membrane potential (Em) and the ouabain-sensitive component of Em in the muscle cells. The findings strengthen the idea that Na,K-ATPase has an important role in regulation of Em in cultured skeletal muscle and suggest that Na-K pump inhibition during development may be a regulatory mechanism for cellular synthesis of Na,K-ATPase.
D000818:Animals; D001665:Binding Sites; D002463:Cell Membrane Permeability; D002478:Cells, Cultured; D004790:Enzyme Induction; D008564:Membrane Potentials / Q000187:drug effects*; D009132:Muscles / Q000187:drug effects*; D010042:Ouabain / Q000494:pharmacology*; D011188:Potassium / Q000378:metabolism; D051381:Rats; D012964:Sodium / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000096:biosynthesis*
D010042:Ouabain; D012964:Sodium; D000254:Sodium-Potassium-Exchanging ATPase; D011188:Potassium