Adenylate kinase activity in Mycobacterium leprae.
Adenylate kinase (ATP:AMP phosphotransferase, EC 2.7.4.3) was detected in partially purified preparations of cell-free extracts of Mycobacterium leprae. The apparent Km values of M. leprae adenylate kinase for ADP and Mg2+ were 1 X 10(-4) M, respectively. The enzyme was heat-labile: loss of activity by 80% at 45 degrees C and over 90% at 60 degrees C occurred within 5 min. M. leprae adenylate kinase was distinct from armadillo adenylate kinase in respect of affinity for substrate and heat-sensitivity.
D000263:Adenylate Kinase / Q000378:metabolism*; D000818:Animals; D001135:Armadillos; D002845:Chromatography; D007700:Kinetics; D009166:Mycobacterium leprae / Q000201:enzymology*; D010770:Phosphotransferases / Q000378:metabolism*
D010770:Phosphotransferases; D000263:Adenylate Kinase