Polypeptide components of bovine heart cytochrome c oxidase. Cross-identifications in two high-resolution polyacrylamide-gel electrophoresis systems.
Two-dimensional polyacrylamide-gel electrophoresis has been used to correlate polypeptide components of bovine heart cytochrome c oxidase that are resolved by two high resolution systems. The systems utilise chloral hydrate (2,2,2-trichloroethane-1,1-diol), which resolves fifteen components, and sodium dodecyl sulphate and urea, which resolves thirteen components. Seven components have been isolated and identified from their amino acid compositions in terms of polypeptides for which the amino acid sequence is known. Full resolution of all components present in this enzyme cannot be accomplished using any single-dimension system currently available.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002417:Cattle; D002697:Chloral Hydrate / Q000494:pharmacology; D003576:Electron Transport Complex IV / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D009206:Myocardium / Q000201:enzymology*; D010455:Peptides / Q000032:analysis*; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D014508:Urea / Q000494:pharmacology
D000596:Amino Acids; D010455:Peptides; D012967:Sodium Dodecyl Sulfate; D002697:Chloral Hydrate; D014508:Urea; D003576:Electron Transport Complex IV