title: Mechanisms of growth inhibition by propionate and restoration of the growth by sodium bicarbonate or acetate in Rhodopseudomonas sphaeroides S.
abstract: Mechanisms of growth inhibition by propionate on the growth of Rhodopseudomonas sphaeroides were studied. Partially purified pyruvate dehydrogenase complex (PDC) from R. sphaeroides was inhibited by propionyl-CoA, one of the metabolic intermediates of propionate, while propionate itself did not inhibit the enzyme. This suggests that the inhibitor of the growth in vivo is not propionate but propionyl-CoA. The inhibition by propionyl-CoA was competitive with respect to coenzyme A concentration. The K1 value for propionyl-CoA was 0.84 mM. Addition of NaHCO3, which restored the growth of this bacterium in the presence of propionate, increased the rate of propionate incorporation by 1.7-fold and decreased the intracellular level of propionyl-CoA by half. These findings suggest that HCO3-ion lowers the level of propionyl-CoA by accelerating its carboxylation reaction, which is catalyzed by propionyl-CoA carboxylase. Effects of NaHCO3 and acetate on the growth restoration were also studied by the use of propionyl-CoA carboxylase-deficient mutants. NaHCO3 did not restore the growth of the mutants, indicating an essential role of propionyl-CoA carboxylase on the restoration of growth by NaHCO3 as suggested above. Addition of acetate restores the growth of the mutants in the presence of propionate. Acetate probably restores the growth by supplying acetyl-CoA.
mesh_terms: D000085:Acetates / Q000494:pharmacology*; D019342:Acetic Acid; D001639:Bicarbonates / Q000494:pharmacology*; D019735:Carbon-Carbon Ligases; D007700:Kinetics; D008025:Ligases / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D011422:Propionates / Q000494:pharmacology*; D011768:Pyruvate Dehydrogenase Complex / Q000378:metabolism; D012242:Rhodobacter sphaeroides / Q000187:drug effects / Q000235:genetics / Q000254:growth & development*; D012964:Sodium / Q000494:pharmacology*; D017693:Sodium Bicarbonate
chemical_list: D000085:Acetates; D001639:Bicarbonates; D011422:Propionates; D011768:Pyruvate Dehydrogenase Complex; D017693:Sodium Bicarbonate; D012964:Sodium; D008025:Ligases; D019735:Carbon-Carbon Ligases; C022458:propionyl CoA carboxylase (ATP-hydrolyzing); C029658:propionic acid; D019342:Acetic Acid