Effect of transformation by Rous sarcoma virus on the character and distribution of actin in Rat-1 fibroblasts: a biochemical and microscopical study.
Actin has been measured in subcellular fractions from Rat-1 fibroblasts and in Rous sarcoma virus-transformed Rat-1 cells (VIT), using the DNase 1 inhibition assay. The transformed cells showed a significant shift in the actin monomer (G)in equilibrium with polymer (F) equilibrium within the cell cytosol, and a significant increase in actin in the Triton-insoluble cytoskeletal core in comparison with untransformed cells. This incorporation of actin into the cytoskeletal core fraction is associated with a change in filamentous actin assemblies from 'stress fibre' patterns to punctate filament aggregates. These differences have been correlated with changes in morphology, in actin, vinculin and alpha-actinin distribution, in adhesion plaque formation and with the production of pp60v-src-associated protein kinase activity in the transformed cells. Changes in actin distribution and its polymerization in response to src-gene expression may play an important role in the determination of the transformed cell characteristics.
D000199:Actins / Q000032:analysis*; D000818:Animals; D001358:Avian Sarcoma Viruses; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000648:ultrastructure*; D003599:Cytoskeleton / Q000648:ultrastructure; D003600:Cytosol / Q000032:analysis; D005347:Fibroblasts / Q000648:ultrastructure*; D005455:Fluorescent Antibody Technique; D008854:Microscopy, Electron; D015688:Oncogene Protein pp60(v-src); D011494:Protein Kinases / Q000378:metabolism; D051381:Rats; D012191:Retroviridae Proteins / Q000378:metabolism
D000199:Actins; D012191:Retroviridae Proteins; D011494:Protein Kinases; D015688:Oncogene Protein pp60(v-src)