Autocrine models of B-lymphocyte growth. II. Interleukin-1 supports the proliferation of transformed lymphoblasts but not the stimulation of resting B cells triggered through their receptors for antigen.
Purified, monocyte-derived interleukin-1 (IL-1) was found to provide growth support for Epstein-Barr virus (EBV) transformed B-lymphocytes seeded at densities below which their own autostimulatory factors were limiting. By contrast, highly purified resting B cells triggered via their receptors for antigen failed to respond to identical preparations of IL-1 by DNA synthesis. That successful priming of the B cells had occurred was evidenced by a transient rise in RNA synthesis and the ability of the cells to respond to T-cell supernatants by DNA synthesis. The findings indicate that while IL-1 might perform an autostimulatory function in B lymphocyte proliferation it is not by itself sufficient to provide growth support for resting B cells activated through their receptors for antigen. The implications of these observations for autocrine models of B-cell growth are discussed.
D000888:Antibodies, Anti-Idiotypic / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology*; D002455:Cell Division; D002460:Cell Line; D002472:Cell Transformation, Viral; D004854:Herpesvirus 4, Human; D006801:Humans; D007148:Immunoglobulin mu-Chains / Q000276:immunology; D007375:Interleukin-1 / Q000276:immunology*; D008213:Lymphocyte Activation; D008954:Models, Biological; D011947:Receptors, Antigen, B-Cell / Q000276:immunology*
D000888:Antibodies, Anti-Idiotypic; D007148:Immunoglobulin mu-Chains; D007375:Interleukin-1; D011947:Receptors, Antigen, B-Cell