Sites of action of brain-gut peptides in cultured neurons of rat brainstem.
Using cultures of dissociated neurons from the lower brainstem of 14- to 15-day-old rat embryos, we studied a site of action of a brain-gut peptide by determining whether neuronal responses to a test peptide are abolished or not after replacement of normal medium with low Ca2+- high Mg2+ medium. VIP, secretin and CCK-4 may act on the postsynaptic membrane, while motilin and neurotensin may act on the presynaptic terminal. Somatostatin and bombesin may work either presynaptically or postsynaptically.
D000818:Animals; D001839:Bombesin / Q000494:pharmacology; D001933:Brain Stem / Q000187:drug effects*; D005768:Gastrointestinal Hormones / Q000494:pharmacology*; D009037:Motilin / Q000494:pharmacology; D009496:Neurotensin / Q000494:pharmacology; D051381:Rats; D012633:Secretin / Q000494:pharmacology; D012844:Sincalide / Q000494:pharmacology; D013569:Synapses / Q000187:drug effects; D009435:Synaptic Transmission / Q000187:drug effects; D013758:Tetragastrin / Q000494:pharmacology; D014660:Vasoactive Intestinal Peptide / Q000494:pharmacology
D005768:Gastrointestinal Hormones; D013758:Tetragastrin; D012633:Secretin; D014660:Vasoactive Intestinal Peptide; D009496:Neurotensin; D009037:Motilin; D012844:Sincalide; D001839:Bombesin