Hepatitis B virus (HBV) DNA in leucocytes in acquired immune deficiency syndrome (AIDS).
Earlier reported findings of hepatitis B virus (HBV) DNA in white blood cells of patients with hepatoma, and in a patient with autoimmune haemolytic anaemia, led to the examination of HBV DNA in a series of twenty three patients with acquired immune deficiency syndrome (AIDS), including nine with opportunistic infections and fourteen with Kaposi's sarcoma, by Southern blot hybridization method, using 32P labelled HBV DNA specific probe obtained by nick translation of HBV DNA cloned into plasmid pBR325. Four of the patients were found to be positive for HBV DNA or HBV related DNA in their leucocytes. The HBV DNA was found free or integrated in the leucocytes of the patients.
D000163:Acquired Immunodeficiency Syndrome / Q000382:microbiology* / Q000473:pathology; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000032:analysis*; D004587:Electrophoresis, Agar Gel; D006515:Hepatitis B virus / Q000032:analysis / Q000235:genetics*; D006801:Humans; D007962:Leukocytes / Q000032:analysis / Q000382:microbiology*; D008297:Male; D009693:Nucleic Acid Hybridization
D004279:DNA, Viral; D004262:DNA Restriction Enzymes