A conserved cis-acting sequence in the 5' leader of avian sarcoma virus RNA is required for packaging.
The deletion of a conserved sequence of ca. 30 nucleotides in the 5' noncoding leader region of an avian sarcoma virus DNA clone resulted in a loss of infectivity after transfection of chicken embryo fibroblasts. Genetic and biochemical analysis of a representative mutant demonstrated that the env gene was expressed normally. Thus, viral RNA transcription, splicing, and translation were not impaired. The amount of mutant viral RNA encapsidated into virions, however, was severely reduced despite the presence of helper-virus. We conclude that the deleted sequence is an essential cis-acting packaging signal.
D000818:Animals; D001358:Avian Sarcoma Viruses / Q000235:genetics* / Q000648:ultrastructure; D002478:Cells, Cultured; D002645:Chickens; D002872:Chromosome Deletion; D004720:Endonucleases; D005796:Genes; D005809:Genes, Regulator; D005814:Genes, Viral; D005816:Genetic Complementation Test; D009024:Morphogenesis; D009154:Mutation; D012367:RNA, Viral / Q000235:genetics*; D015719:Single-Strand Specific DNA and RNA Endonucleases; D014162:Transfection; D014759:Viral Envelope Proteins / Q000235:genetics
D012367:RNA, Viral; D014759:Viral Envelope Proteins; D004720:Endonucleases; D015719:Single-Strand Specific DNA and RNA Endonucleases