Analysis of the origin-specific nucleosome-free region in SV40 encapsidation intermediates.
The presence of a nucleosome-free region at the replication origin in encapsidation intermediates of SV40 was determined by measuring the sensitivity of the intermediates to digestion by restriction endonucleases which lie within (BglI and SphI) or outside of this region (KpnI, MspI, and EcoRI). As judged by hypersensitivity to digestion with BglI and SphI, the origin-specific nucleosome-free region was found to be present in nonencapsidated and partially encapsidated SV40 chromosomes and previrions, but not virions.
D002213:Capsid / Q000378:metabolism; D002875:Chromosomes / Q000032:analysis; D004261:DNA Replication; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000032:analysis; D009707:Nucleosomes; D013539:Simian virus 40 / Q000235:genetics*; D013936:Thymidine / Q000378:metabolism; D014316:Tritium; D014779:Virus Replication
D004279:DNA, Viral; D009707:Nucleosomes; D014316:Tritium; D004262:DNA Restriction Enzymes; D013936:Thymidine