Isolation of a cDNA clone for the human muscle specific carbonic anhydrase, CAIII.
The molecular cloning of cDNA for the human muscle specific carbonic anhydrase CAIII is described. The recombinant was isolated from a human muscle cDNA library prepared in the expression vector lambda gt11, and was characterized by hybridization selection and immunoprecipitation. A comparison of insert cDNA and mRNA sizes suggests that the cDNA is full length and includes extensive untranslated sequences. Preliminary sequence data have confirmed the authenticity of this clone and Southern blotting of human and rodent DNA indicates that it will be a useful probe in the analysis of somatic cell hybrids.
D000818:Animals; D010582:Bacteriophage lambda / Q000235:genetics; D001483:Base Sequence; D002256:Carbonic Anhydrases / Q000235:genetics*; D003001:Cloning, Molecular; D006224:Cricetinae; D004247:DNA / Q000235:genetics / Q000302:isolation & purification*; D004262:DNA Restriction Enzymes; D004587:Electrophoresis, Agar Gel; D006801:Humans; D006822:Hybrid Cells; D051379:Mice; D009132:Muscles / Q000201:enzymology*; D051381:Rats
D004247:DNA; D004262:DNA Restriction Enzymes; D002256:Carbonic Anhydrases