An electroblotting technique for assessing the integrity of the major immunogenic protein in foot-and-mouth disease virus vaccines.
A method has been developed whereby VP1, the major immunogenic protein of foot-and-mouth disease virus can be detected after electroblotting on nitrocellulose paper. Proteins can be examined in unfractionated virus harvests and after formulation as aluminium hydroxide-adjuvanted vaccines. The limit of detection is approximately 10 ng of VP1 and up to 20 samples can be analysed simultaneously. The technique allows the integrity of VP1 to be examined in fully formulated vaccines.
D000818:Animals; D005537:Aphthovirus / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D007158:Immunologic Techniques; D010455:Peptides / Q000032:analysis / Q000276:immunology; D014764:Viral Proteins / Q000032:analysis* / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology*
D010455:Peptides; D014764:Viral Proteins; D014765:Viral Vaccines