Prostaglandins, cyclic AMP production and biological activity of alloimmune thymocytes.
The effect of alloimmunized non-adherent thymocytes on the spontaneous activity of the mouse atria was studied. BALB/c anti C3H non-adherent thymocytes induced negative inotropic effect on C3H atria. Cell-free supernatant from non-adherent thymocytes induced the same biological activity. This activity was blunted by the inhibition of non-adherent immune thymocytes' cyclo-oxygenase activity. PGE was present in higher amounts in free-cell supernatant from BALB/c anti C3H thymocytes plus C3H atria than in those from non-immune thymocytes. Intracellular levels of immune thymocytes cAMP are raised in comparison with those of normal thymocytes. It is proposed that alloantigen stimulates non-adherent thymocytes, increasing intracellular levels of cAMP and PGE. Immune thymocytes, release PGE upon recognition of the alloantigens expressed in the atria and this triggers a negative inotropic effect. The increment in immune thymocyte cAMP appears to be associated with the activation of thymocytes cyclo-oxygenase activity by alloimmunization.
D000818:Animals; D000920:Antibody-Dependent Cell Cytotoxicity; D002448:Cell Adhesion; D000242:Cyclic AMP / Q000096:biosynthesis*; D007519:Isoantigens; D008264:Macrophages / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains; D009200:Myocardial Contraction; D009206:Myocardium / Q000276:immunology; D011458:Prostaglandins E / Q000096:biosynthesis*; D013601:T-Lymphocytes / Q000276:immunology; D013950:Thymus Gland / Q000276:immunology* / Q000378:metabolism
D007519:Isoantigens; D011458:Prostaglandins E; D000242:Cyclic AMP