Lateral diffusion of lipid probes in the surface membrane of human platelets. An electron-electron double resonance (ELDOR) study.
Electron-electron double resonance (ELDOR) techniques employing [14N], [15N] 16-Doxylstearate spin-label pairs have been used to measure the lateral diffusion constant, D, of lipids in the surface membrane of intact human blood platelets. For freshly prepared platelets, D is 1.0 X 10(-8) cm2/s at 37 degrees C and for platelets stored for 3 d at room temperature under accepted routine blood bank conditions, D is 2.6 X 10(-8) cm2/s at 37 degrees C. This is the first time that D in the surface membrane of platelets is reported. The marked increase in D for stored platelets may be attributed at least partly to loss of cholesterol during storage, suggesting a correlation between lipid lateral diffusion and cholesterol levels in cell membranes.
D001792:Blood Platelets / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D003497:Cyclic N-Oxides; D004058:Diffusion; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods; D006801:Humans; D007700:Kinetics; D008563:Membrane Lipids / Q000097:blood
D003497:Cyclic N-Oxides; D008563:Membrane Lipids; C032363:16-nitroxystearic acid