Proton translocation by cytochrome oxidase vesicles catalyzing the peroxidatic oxidation of ferrocytochrome c.
Coupled with the peroxidatic oxidation of ferrocytochrome c under anaerobic conditions, proteoliposomes reconstituted with a purified preparation of bovine heart cytochrome oxidase ejected protons into the external medium with an apparent H+/e- ratio of 0.9. At the same time, protons in the intravesicular space were consumed. Dicyclohexylcarbodiimide significantly inhibited the proton translocation. Cyanide (0.14 mM) completely inhibited both the peroxidase and proton translocating activities. On the contrary, in the presence of 1 mM CO the proton ejection was abolished almost completely, but 50% of the peroxidase activity persisted. This result suggests the operation of multiple mechanisms in the peroxidase reaction and that the CO-sensitive one is coupled to the proton translocation.
D000818:Animals; D002248:Carbon Monoxide / Q000494:pharmacology; D002417:Cattle; D003486:Cyanides / Q000494:pharmacology; D003574:Cytochrome c Group / Q000378:metabolism*; D004024:Dicyclohexylcarbodiimide / Q000494:pharmacology; D003576:Electron Transport Complex IV / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D009206:Myocardium / Q000201:enzymology; D010545:Peroxides / Q000378:metabolism*; D011510:Proteolipids / Q000378:metabolism; D011522:Protons
D003486:Cyanides; D003574:Cytochrome c Group; D010545:Peroxides; D011510:Proteolipids; D011522:Protons; C020485:proteoliposomes; D004024:Dicyclohexylcarbodiimide; D002248:Carbon Monoxide; D003576:Electron Transport Complex IV