title: Interaction of vasoactive intestinal peptide (VIP) with human peripheral blood lymphocytes: specific binding and cyclic AMP production.
abstract: VIP binding sites and cyclic AMP production by the peptide have been studied in human blood mononuclear cells before and after selective depletion of or enrichment for T-lymphocytes, B-lymphocytes-K-NK cells and monocytes. The specifically bound 125I-labelled VIP correlated significantly with the presence of B-lymphocytes and/or cells of K-NK system. The stoichiometric data were compatible with the existence of two classes of binding sites. T-lymphocytes and monocytes did not show binding of the tracer. The cyclic AMP production stimulated by VIP correlated significantly with the presence of B-lymphocytes and/or K-NK cells.
mesh_terms: D000328:Adult; D001402:B-Lymphocytes / Q000378:metabolism; D001665:Binding Sites; D000242:Cyclic AMP / Q000096:biosynthesis*; D006801:Humans; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000378:metabolism*; D013601:T-Lymphocytes / Q000378:metabolism; D014660:Vasoactive Intestinal Peptide / Q000378:metabolism*
chemical_list: D014660:Vasoactive Intestinal Peptide; D000242:Cyclic AMP