Stimulation of superoxide release in neutrophils by 1-oleoyl-2-acetylglycerol incorporated into pH-sensitive liposomes.
Incorporation of 1-oleoyl-2-acetylglycerol (OAG) into multilamellar liposomes composed of egg phosphatidylethanolamine (PE) and arachidonic acid (AA) resulted in a significant enhancement of superoxide release by guinea pig neutrophils when compared to free OAG. OAG incorporated into liposomes containing phosphatidylcholine and arachidonic acid were generally less effective than free OAG. The potency of the liposomes correlates well with the ability of the liposomes to undergo lipid mixing at acidic pH. The enhanced effect of liposome-associated OAG could be related to exposure to an acidic environment in the endosomes/lysosomes once liposomes are endocytosed by neutrophils.
D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids; D004075:Diglycerides / Q000008:administration & dosage / Q000494:pharmacology*; D005453:Fluorescence; D005989:Glycerides / Q000494:pharmacology*; D006168:Guinea Pigs; D006863:Hydrogen-Ion Concentration; D008081:Liposomes; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D010714:Phosphatidylethanolamines; D011493:Protein Kinase C / Q000378:metabolism; D013481:Superoxides / Q000097:blood*
D001095:Arachidonic Acids; D004075:Diglycerides; D005989:Glycerides; D008081:Liposomes; D010714:Phosphatidylethanolamines; D013481:Superoxides; D016718:Arachidonic Acid; C038237:1-oleoyl-2-acetylglycerol; D011493:Protein Kinase C