Quantitative autoradiographic determination of angiotensin-converting enzyme (kininase II) binding in individual rat brain nuclei with 125I-351A, a specific enzyme inhibitor.
We report the localization and characterization of angiotensin-converting enzyme (kininase II) in discrete nuclei from individual rat brains by a quantitative autoradiographic technique coupled to computerized microdensitometry. The enzyme was quantitated by incubation of 16-micron-thick brain sections with 0.07-2 nM of the converting enzyme inhibitor 125I-351A and comparison to 125I-standards. This technique can be applied to the study of other enzymes in single rat brain nuclei.
D000818:Animals; D001345:Autoradiography; D001665:Binding Sites; D001921:Brain / Q000378:metabolism*; D002831:Choroid Plexus / Q000378:metabolism; D003342:Corpus Striatum / Q000378:metabolism; D004151:Dipeptides / Q000378:metabolism*; D008297:Male; D007703:Peptidyl-Dipeptidase A / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013356:Subfornical Organ / Q000378:metabolism
D004151:Dipeptides; C042037:L-proline, N2-((1S)-1-carboxy-3-phenylpropyl)-N6-((4-hydroxyphenyl)iminomethyl)-L-lysyl-; D007703:Peptidyl-Dipeptidase A