Construction of frameshift mutation hot spots within the tetracycline resistance gene of pBR322.
The chemical carcinogen, N-2-acetylaminofluorene (AAF) when bound covalently to DNA induces a majority (greater than 90%) of frameshift mutations. The mutations occur with high frequencies at defined sequences (i.e. mutation hot spots). Two classes of mutation hot spots were found: at repetitive sequences and at specific non-repetitive sequences. Mutations at the repetitive sequences depend upon a functional umuC gene whereas mutations at specific non-repetitive sequences are umuC-independent. The first discovered sequence of this class is the NarI restriction enzyme recognition sequence (5'GGCGCC3'). In an attempt to define a family of such sequences we constructed a related sequence 5'GCGCGC3' within the tetracycline resistance gene of pBR322. This sequence was also found to be an--AAF induced--2 frameshift mutation hot spot in both wild type and umuC strains.
D015073:2-Acetylaminofluorene / Q000494:pharmacology; D001483:Base Sequence; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000235:genetics / Q000528:radiation effects; D015252:Deoxyribonucleases, Type II Site-Specific; D005798:Genes, Bacterial; D009154:Mutation; D011815:R Factors; D012091:Repetitive Sequences, Nucleic Acid; D013752:Tetracycline; D014466:Ultraviolet Rays
D004269:DNA, Bacterial; D015073:2-Acetylaminofluorene; D004262:DNA Restriction Enzymes; C044171:endodeoxyribonuclease BSSHII; D015252:Deoxyribonucleases, Type II Site-Specific; C046719:GGCGCC-specific type II deoxyribonucleases; D013752:Tetracycline