Preferential transcription of HTLV-I LTR in cell-free extracts of human T cells producing HTLV-I viral proteins.
The promoters of the adenovirus 2 major late gene, the mouse beta-globin gene, the mouse immunoglobulin VH gene and the LTR of the human T-lymphotropic retrovirus type I were tested for their transcription activities in cell-free extracts of four cell lines; HeLa, CESS (Epstein-Barr virus-transformed human B cell line), MT-1 (HTLV-I-infected human T cell line without viral protein synthesis), and MT-2 (HTLV-I-infected human T cell line producing viral proteins). LTR was preferentially transcribed in the extracts of MT-2 although the other three genes were transcribed with relatively constant efficiencies in different extracts. The results agree well with the previous in vivo studies on the promoter activity of HTLV-I LTR. Mixing of HeLa and MT-2 extracts revealed the presence of a LTR-specific stimulating activity in MT-2 extracts.
D002460:Cell Line; D002474:Cell-Free System; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics*; D017977:Deltaretrovirus / Q000235:genetics*; D005786:Gene Expression Regulation; D006367:HeLa Cells / Q000502:physiology; D006801:Humans; D011401:Promoter Regions, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D013601:T-Lymphocytes / Q000382:microbiology*; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000096:biosynthesis / Q000235:genetics
D004279:DNA, Viral; D014157:Transcription Factors; D014764:Viral Proteins