[Synthesis and cloning of a DNA fragment containing a probable site for eukaryotic mRNA binding to ribosome].
A series of oligonucleotides, including two polynucleotides of 33 bases long, were synthesized by a solid-phase phosphotriester method. Potassium salt of 3-nitro-1,2,4-triazole in the presence of 18-crown-6 ether was used as nucleophilic catalyst. The partly complementary polynucleotides were elongated by DNA-polymerase I (Klenow fragment) to the full duplex, which was digested with SalGI and was inserted into a plasmid pUR222. Phe synthesized DNA fragment precedes the gene of human gamma-interferon in the chromosome and contains the site for mRNA binding to ribosome.
D001483:Base Sequence; D001665:Binding Sites; D002852:Chromatography, Ion Exchange; D003001:Cloning, Molecular; D004247:DNA / Q000138:chemical synthesis / Q000235:genetics*; D004262:DNA Restriction Enzymes; D005057:Eukaryotic Cells; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics*; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000235:genetics; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism*
D009841:Oligonucleotides; D012333:RNA, Messenger; D007371:Interferon-gamma; D004247:DNA; D004262:DNA Restriction Enzymes
Sintez i klonirovanie fragmenta DNK, soderzhashchego predpolagaemyĭ saĭt sviazyvaniia éukarioticheskoĭ mRNK s ribosomoU