Additional c-abl/bcr rearrangements in a CML patient exhibiting two ph1 chromosomes during blast crisis.
Recent data suggest that two human genes, c-abl on chromosome 9 and bcr on chromosome 22, are involved in the generation of Ph1-positive CML. To examine a possible role of these sequences in transition from chronic towards blastic phase, rearrangements within bcr were analysed in 4 patients with Ph1-positive CML during chronic and acute phase. In 3 patients bcr rearrangements were identical in both phases, while in a fourth patient with duplicated Ph1 an amplified additional bcr fragment was detected in acute phase. Northern blot analysis of blast cells of the latter patient showed a novel 10.3 kb RNA species that replaced the altered 8 kb RNA transcript usually found in Ph1-positive CML.
D000293:Adolescent; D001853:Bone Marrow / Q000473:pathology; D002869:Chromosome Aberrations; D002906:Chromosomes, Human, 6-12 and X; D004262:DNA Restriction Enzymes; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D006801:Humans; D007951:Leukemia, Myeloid / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009693:Nucleic Acid Hybridization; D009857:Oncogenes; D010677:Philadelphia Chromosome; D012334:RNA, Neoplasm / Q000032:analysis
D004273:DNA, Neoplasm; D012334:RNA, Neoplasm; D004262:DNA Restriction Enzymes