Babesia bovis: purification and concentration of merozoites and infected bovine erythrocytes.
Babesia bovis merozoites, externalized by removal of infected erythrocytes from ordinary culture conditions, were completely separated from red blood cells and stroma by centrifugation in a Percoll gradient. A merozoite band formed at a point corresponding to about 1.087 g/ml specific density. Infected red blood cells were concentrated approximately fourfold to obtain greater than 49.0% parasitemia after centrifugation in Percoll. Most highly enriched fractions positioned between 1.121 and 1.123 g/ml specific density. Full parasite viability was retained.
D000818:Animals; D001403:Babesia / Q000166:cytology / Q000302:isolation & purification*; D001404:Babesiosis / Q000097:blood*; D002417:Cattle; D002499:Centrifugation, Density Gradient / Q000379:methods; D004912:Erythrocytes / Q000469:parasitology*; D011205:Povidone; D012822:Silicon Dioxide
C016039:Percoll; D012822:Silicon Dioxide; D011205:Povidone