title: Modification of brain fructose-1,6-bisphosphatase activity by chelators: "induction" of 5'-AMP sensitivity.
abstract: Brain fructose-1,6-bisphosphatase (EC 3.1.3.11) from various sources are ordinarily insensitive to 5'-AMP. In addition to stimulation and conferring a "neutral" behaviour, prior treatment with histidine, EDTA or imidazole renders the brain enzyme sensitive to 5'-AMP. The histidine treated enzyme(s) bind to Blue-Sepharose CL-6B column and are specifically eluted by 5'-AMP in contrast to the untreated enzyme(s) which do not bind to the affinity column at all. The histidine effect in inducing 5'-AMP sensitivity was abolished by treatment of the native enzyme by subtilisin or by a number of divalent cations including Zn++.
mesh_terms: D000249:Adenosine Monophosphate / Q000494:pharmacology*; D000818:Animals; D001665:Binding Sites; D001921:Brain / Q000201:enzymology*; D002413:Cations, Divalent / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D005399:Fishes; D006597:Fructose-Bisphosphatase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D006639:Histidine / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007093:Imidazoles / Q000494:pharmacology; D051381:Rats; D013381:Subtilisins / Q000378:metabolism; D015032:Zinc / Q000494:pharmacology
chemical_list: D002413:Cations, Divalent; D007093:Imidazoles; D000249:Adenosine Monophosphate; D006639:Histidine; C029899:imidazole; D004492:Edetic Acid; D006597:Fructose-Bisphosphatase; D013381:Subtilisins; D015032:Zinc