title: camR, a negative regulator locus of the cytochrome P-450cam hydroxylase operon.
abstract: A 4.27-kilobase insert from a HindIII DNA library of Pseudomonas putida carrying the CAM plasmid allowed coordinate expression of genes camD and camC under control of camR, an upstream regulator. The camC gene specifies cytochrome P-450cam, and camD specifies the 5-exo-alcohol dehydrogenase. A 1.38-kilobase deletion from the insert results in the constitutive expression of genes camC and camD; transformation in trans restores the substrate control, indicating that camR is a negative regulator.
mesh_terms: D000426:Alcohol Dehydrogenase; D000429:Alcohol Oxidoreductases / Q000235:genetics; D000595:Amino Acid Sequence; D001483:Base Sequence; D002872:Chromosome Deletion; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D004262:DNA Restriction Enzymes / Q000378:metabolism; D015247:Deoxyribonuclease HindIII; D005786:Gene Expression Regulation; D005796:Genes; D005798:Genes, Bacterial; D005809:Genes, Regulator; D007527:Isoenzymes / Q000235:genetics*; D009876:Operon; D010957:Plasmids; D011549:Pseudomonas / Q000201:enzymology / Q000235:genetics
chemical_list: D007527:Isoenzymes; D003577:Cytochrome P-450 Enzyme System; D000429:Alcohol Oxidoreductases; D000426:Alcohol Dehydrogenase; D004262:DNA Restriction Enzymes; D015247:Deoxyribonuclease HindIII