title: [Activation of ras and myc proto-oncogenes in human breast carcinoma and neuroblastoma].
abstract: DNA from human breast carcinoma (SK-BR-3) and neuroblastoma (LA-N-1) cell lines are capable of inducing foci of transformed NIH 3T3 cells after DNA-mediated gene transfer. The blot hybridization analysis of DNA from primary and secondary NIH 3T3 transformants identified additional sequences homologous to the c-Ha-ras 1 oncogene, and revealed amplification of nucleotide sequences homologous to the v-myc oncogene. Restriction fragments of the amplified myc-related sequences correspond to c-myc (SK-BR-3) and N-myc (LA-N-1) loci of the human genome. The results show that active Ha-ras oncogenes can coexist with altered myc oncogenes in breast carcinomas and neuroblastomas. This suggests that a multi-step mechanism involves both ras and myc genes and their cooperation in the development of these tumors.
mesh_terms: D000818:Animals; D001943:Breast Neoplasms / Q000235:genetics*; D002460:Cell Line; D004262:DNA Restriction Enzymes; D004273:DNA, Neoplasm / Q000235:genetics*; D005784:Gene Amplification; D006801:Humans; D051379:Mice; D009447:Neuroblastoma / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D011519:Proto-Oncogenes; D012689:Sequence Homology, Nucleic Acid; D014162:Transfection
chemical_list: D004273:DNA, Neoplasm; D004262:DNA Restriction Enzymes
vernacular_title: Aktivatsiia protoonkogenov iz semeĭstva ras i myc v kartsinome molochnoĭ zhelezy i neĭroblastome cheloveka.