Physiological role of putative testicular gonadotrophin releasing hormone (GnRH).
Evidence suggests that exogenous GnRH and agonist analogues have short-term stimulatory effects on rat Leydig cell function - when administered intratesticularly. Since rat Leydig cells possess GnRH receptors and their endogenous ligand has not yet been identified the physiological importance of the observations for testis function is unknown. To address this issue we have determined the consequences of blockade of testis GnRH receptors on Leydig cell function under both normogonadotrophic and hypogonadotrophic stimulation of the testis in vivo. A GnRH antagonist (ANT) was used to achieve receptor blockade but during continuous systemic infusion ANT occupied pituitary GnRH receptors and markedly reduced serum LH, FSH, testosterone, and intratesticular testosterone in adult and 30 d old immature male rats. These results were similar to those obtained by administration of a GnRH antiserum which did not bind to testis GnRH receptors. Thus, blockade of testis GnRH receptors during hypogonadotrophism did not produce additional inhibition of steroidogenesis by Leydig cells. However, direct continuous infusion of ANT into one testis produced greater than 90% occupancy of GnRH receptors while reducing GnRH receptors by only 50% in the contralateral testis. Unilateral intratesticular infusion did not reduce serum LH, FSH, Prolactin or testosterone levels despite 75% occupancy of pituitary GnRH receptors. Thus, both ANT infused and saline infused testes were exposed to the same gonadotrophic stimulants but in the former GnRH-R were essentially non-existent. Compared to the control testis, the ANT infused testis showed a 20-30% reduction in LH, FSH, lactogen receptors and 30-40% fall in testosterone content. Identical results were obtained in adult and 30 d-old male rats.(ABSTRACT TRUNCATED AT 250 WORDS)
D000818:Animals; D005640:Follicle Stimulating Hormone / Q000097:blood; D007985:Leydig Cells / Q000032:analysis / Q000378:metabolism; D007986:Luteinizing Hormone / Q000097:blood; D008297:Male; D010902:Pituitary Gland / Q000032:analysis; D010905:Pituitary Hormone Release Inhibiting Hormones / Q000494:pharmacology*; D010906:Pituitary Hormone-Releasing Hormones / Q000502:physiology*; D011388:Prolactin / Q000097:blood; D051381:Rats; D011956:Receptors, Cell Surface / Q000032:analysis / Q000187:drug effects*; D011962:Receptors, FSH; D011974:Receptors, LH; D011981:Receptors, Prolactin; D013735:Testicular Hormones / Q000502:physiology*; D013737:Testis / Q000187:drug effects; D013739:Testosterone / Q000097:blood
D010905:Pituitary Hormone Release Inhibiting Hormones; D010906:Pituitary Hormone-Releasing Hormones; D011956:Receptors, Cell Surface; D011962:Receptors, FSH; D011974:Receptors, LH; D011981:Receptors, Prolactin; D013735:Testicular Hormones; D013739:Testosterone; D011388:Prolactin; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone