Phosphatidylinositol turnover in neuroblastoma cells: regulation by bradykinin, acetylcholine, but not mu- and delta-opioid receptors.
The effect of opioids on phosphatidylinositol (PI) turnover to release inositol triphosphate (IP3) as second messenger was examined in mouse neuroblastoma X rat glioma hybrid cells NG108-15 (delta-receptors) and human neuroblastoma cells, SK-N-SH (predominantly mu-receptors). PI turnover can be stimulated in both NG108-15 and SK-N-SH cells by bradykinin and acetylcholine, respectively. In contrast, etorphine, DADL ([D-Ala2,D-Leu5]-enkephalin) and DAGO ([D-Ala2,MePhe4,Gly-ol5]-enkephalin), up to 1 microM concentrations failed to affect PI turnover in both cell lines. These results suggest that IP3 is not likely to serve as second messenger for both mu- and delta-opioid receptors.
D000109:Acetylcholine / Q000494:pharmacology*; D000818:Animals; D001920:Bradykinin / Q000494:pharmacology*; D002460:Cell Line; D020875:Enkephalin, Ala(2)-MePhe(4)-Gly(5)-; D004743:Enkephalin, Leucine / Q000031:analogs & derivatives / Q000494:pharmacology; D016308:Enkephalin, Leucine-2-Alanine; D004745:Enkephalins / Q000494:pharmacology; D005048:Etorphine / Q000494:pharmacology; D051379:Mice; D010716:Phosphatidylinositols / Q000378:metabolism*; D051381:Rats; D011957:Receptors, Opioid / Q000502:physiology*
D004745:Enkephalins; D010716:Phosphatidylinositols; D011957:Receptors, Opioid; D020875:Enkephalin, Ala(2)-MePhe(4)-Gly(5)-; D005048:Etorphine; D004743:Enkephalin, Leucine; D016308:Enkephalin, Leucine-2-Alanine; D000109:Acetylcholine; D001920:Bradykinin