title: DNA engineering shows that nucleosome phasing on the African green monkey alpha-satellite is the result of multiple additive histone-DNA interactions.
abstract: The mechanism underlying sequence-specific positioning of nucleosomes on DNA was investigated. African green monkey alpha-satellite DNA was reconstituted in vitro with histones. Histone octamers were found to adopt one major and several minor positions on the satellite repeat unit, very similar to those positions found previously in vitro, demonstrating that sequence-specific histone-DNA interactions are responsible for nucleosome positioning on this DNA. In order to understand the nature of these interactions in more detail, we have constructed a variant satellite fragment containing an insertion of half a helical DNA turn. The parent fragment directs histones to one major and two overlapping minor positions that are all affected by the insertion. All three frames respond in a unique fashion to the additional five base-pairs. From a quantitative analysis of the nucleosome positions on the engineered fragment, consensus "phasing boxes" as the basis for nucleosome positioning can be ruled out. Instead, our results argue very strongly that nucleosome positioning is due to the independent contribution of many different DNA-histone contacts along the entire core particle, in an apparently additive fashion.
mesh_terms: D000818:Animals; D001482:Base Composition; D001483:Base Sequence; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D004276:DNA, Satellite / Q000378:metabolism*; D005818:Genetic Engineering; D006657:Histones / Q000378:metabolism*; D009707:Nucleosomes / Q000378:metabolism*
chemical_list: D004251:DNA Transposable Elements; D004276:DNA, Satellite; D006657:Histones; D009707:Nucleosomes