Use of specific collagenases for the isolation of rat liver cells with preserved lipase activities.
The heparin-releasable neutral lipase (EC 3.1.1.3) from rat liver is inactivated by the common preparations of collagenases (EC 3.4.24.3) used for the isolation of liver cells. We show that two collagenases purified from Clostridium histolyticum allow both the complete preservation of this lipolytic activity and a good viability of liver cells isolated by the usual perfusion protocol.
D000818:Animals; D002458:Cell Fractionation / Q000379:methods*; D003013:Clostridium / Q000201:enzymology; D008049:Lipase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D008099:Liver / Q000166:cytology / Q000201:enzymology*; D008297:Male; D003012:Microbial Collagenase / Q000302:isolation & purification / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains
D008049:Lipase; D003012:Microbial Collagenase