Activated c-raf gene in a rat hepatocellular carcinoma induced by 2-amino-3-methylimidazo[4,5-f]quinoline.
A rat hepatocellular carcinoma, IQ7, induced by 2-amino-3-methylimidazo[4,5-f]quinoline (IQ) gave two transformants of NIH 3T3 cells on DNA mediated gene transfer. One of these transformants was examined further and secondary and tertiary transformants were obtained. The secondary transformant was tumorigenic in nude mice. The activated oncogene in this primary transformant was identified as rat c-raf by Southern blot analysis.
D000818:Animals; D001483:Base Sequence; D002471:Cell Transformation, Neoplastic; D004262:DNA Restriction Enzymes / Q000378:metabolism; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced / Q000235:genetics*; D051379:Mice; D008819:Mice, Nude; D009857:Oncogenes; D011804:Quinolines; D051381:Rats; D011916:Rats, Inbred F344; D012091:Repetitive Sequences, Nucleic Acid
D011804:Quinolines; C029216:2-amino-3-methylimidazo(4,5-f)quinoline; D004262:DNA Restriction Enzymes