Initiation of phage phi 29 DNA replication by mutants with deletions at the carboxyl end of the terminal protein.
Series of deletions at the C end of p3, the phage phi 29 DNA terminal protein (TP), have been constructed and characterized. Measurements of the activity of those deletion mutants in the formation of the p3-dAMP initiation complex in vitro indicate the need of an intact C-end for the normal TP primer function in DNA replication. It appears that the region at the C-end between aa 240 and 262 of p3, or part of it, might be essential for the normal TP function.
D000595:Amino Acid Sequence; D002872:Chromosome Deletion; D003090:Coliphages / Q000235:genetics*; D004261:DNA Replication; D004262:DNA Restriction Enzymes; D004926:Escherichia coli / Q000235:genetics*; D005796:Genes; D005814:Genes, Viral; D009154:Mutation; D010957:Plasmids; D014764:Viral Proteins / Q000235:genetics*
D014764:Viral Proteins; C040784:terminal protein, Bacillus phage phi29; D004262:DNA Restriction Enzymes