Molecular analysis of chemically-induced mutations at the RpII215 locus of Drosophila melanogaster.
A substantial fraction, perhaps 50% or more, of spontaneous mutations in Drosophila melanogaster have been shown by molecular analyses to be associated with the presence of a transposable element (TE) inserted into the affected gene. We are interested in the molecular structure of induced mutations in Drosophila, in particular whether TEs are also responsible for a significant proportion of chemically-induced mutations. We report here the molecular analysis of 58 mutations at the RpII215 locus induced with EMS or ENU. While we find evidence for moderately sized deletions at this locus (in 3/58, or 5% of the examined mutants), we failed to detect any mutations which were associated with an insertion event. It may be the case that induced mutations are qualitatively different from spontaneous mutations.
D000818:Animals; D002872:Chromosome Deletion; D002874:Chromosome Mapping; D004262:DNA Restriction Enzymes; D004251:DNA Transposable Elements; D004331:Drosophila melanogaster / Q000235:genetics*; D005020:Ethyl Methanesulfonate / Q000633:toxicity; D005038:Ethylnitrosourea / Q000633:toxicity; D009153:Mutagens; D009154:Mutation
D004251:DNA Transposable Elements; D009153:Mutagens; D005020:Ethyl Methanesulfonate; D004262:DNA Restriction Enzymes; D005038:Ethylnitrosourea