A novel, simple and rapid method for the isolation of mitochondria which exhibit respiratory control, from rat small intestinal mucosa.
A method is described for the isolation of functional mitochondria from rat intestinal mucosa. Its novel feature is the removal of mucus from the initial homogenate by treatment with DEAE-cellulose. The preparations exhibited acceptable ADP:O ratios, high State-3 respiration rates, and respiratory control ratios in excess of 3 when succinate, beta-hydroxybutyrate, glutamate/malate and glutamine were test substrates.
D000818:Animals; D002417:Cattle; D002458:Cell Fractionation / Q000379:methods; D002848:Chromatography, DEAE-Cellulose; D003576:Electron Transport Complex IV / Q000378:metabolism; D007413:Intestinal Mucosa / Q000648:ultrastructure*; D007421:Intestine, Small / Q000201:enzymology*; D008297:Male; D008928:Mitochondria / Q000502:physiology*; D009093:Mucus / Q000032:analysis; D010085:Oxidative Phosphorylation; D010101:Oxygen Consumption; D051381:Rats
D003576:Electron Transport Complex IV