Measurement and activity of cytosolic deoxyribonucleoside-activated nucleotidase in various cell types from rat liver and spleen.
The enzyme activity was measured in hepatocytes, Kupffer cells, endothelial cells and spleen cells. Hepatocytes showed proportionality between enzyme activity and cytosol concentration, but with Kupffer cells, endothelial cells and spleen cells the specific activity decreased with decreasing cytosol concentration when the amount of cytosol protein in 250 microliters incubation mixture was below 80, 60 and 20 micrograms, respectively. The specific activities in hepatocytes, Kupffer cells, endothelial cells and spleen cells were 2, 16, 18 and 115 nmol/min per mg of cytosol protein, respectively.
D000818:Animals; D003600:Cytosol / Q000201:enzymology; D004727:Endothelium / Q000201:enzymology; D007700:Kinetics; D007728:Kupffer Cells / Q000201:enzymology; D008099:Liver / Q000166:cytology / Q000201:enzymology*; D008297:Male; D009708:Nucleotidases / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013154:Spleen / Q000166:cytology / Q000201:enzymology*
D009708:Nucleotidases; C030287:nucleotidase