Neutralization of the AIDS retrovirus by antibodies to a recombinant envelope glycoprotein.
Mammalian cell lines have been engineered to produce a secreted form of the AIDS retrovirus envelope glycoprotein. The recombinant protein has been isolated from growth-conditioned culture media and used to immunize animals. Antibodies directed against the recombinant molecule were found to react with the envelope glycoprotein produced in virus-infected cells. Furthermore, these antibodies were able to directly inactivate the AIDS retrovirus in a neutralization assay in vitro. The expression system reported here should provide sufficient quantities of the AIDS retrovirus envelope protein for biological and vaccination studies.
D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology*; D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology*; D000956:Antigens, Viral / Q000096:biosynthesis / Q000276:immunology*; D006224:Cricetinae; D017977:Deltaretrovirus / Q000276:immunology*; D006168:Guinea Pigs; D015483:HIV Antibodies; D015488:HIV Antigens; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D008297:Male; D009500:Neutralization Tests; D011817:Rabbits; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000276:immunology; D014759:Viral Envelope Proteins / Q000096:biosynthesis / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology
D000914:Antibodies, Viral; D000956:Antigens, Viral; D015483:HIV Antibodies; D015488:HIV Antigens; D007106:Immune Sera; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins; D014765:Viral Vaccines