Translational coupling in Escherichia coli of a heterologous Bacillus subtilis-Escherichia coli gene fusion.
The efficient expression in Escherichia coli of the Tn9-derived chloramphenicol acetyltransferase (EC 2.3.1.28) gene fused distal to the promoter and N terminus of the Bacillus subtilis aprA gene was dependent on the initiation of translation from the ribosome-binding site in the aprA gene.
D000123:Acetyltransferases / Q000235:genetics; D001412:Bacillus subtilis / Q000235:genetics*; D015500:Chloramphenicol O-Acetyltransferase; D004251:DNA Transposable Elements; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D011401:Promoter Regions, Genetic; D014176:Protein Biosynthesis; D012270:Ribosomes / Q000378:metabolism; D013381:Subtilisins / Q000235:genetics
D004251:DNA Transposable Elements; D004274:DNA, Recombinant; D000123:Acetyltransferases; D015500:Chloramphenicol O-Acetyltransferase; D013381:Subtilisins