Serotonin-1C sites in the choroid plexus are not linked in a stimulatory or inhibitory way to adenylate cyclase.
The association of the serotonin recognition sites in the pig choroid plexus (5-HT-1C sites) with an adenylate cyclase was examined. The interaction of serotonin and mianserin with [3H]mesulergine binding was not affected by the stable GTP analogue GppNHp. The binding of [3H]serotonin to choroid plexus membranes was also unaffected by GppNHp while a dose-dependent decrease was observed in pig cortical and hippocampal membranes. The porcine choroid plexus contained a forskolin- and histamine-sensitive adenylate cyclase. Serotonin, however, was ineffective in this preparation. While forskolin-stimulated adenylate cyclase in the rat hippocampus was inhibited by serotonin forskolin-stimulated adenylate cyclase in the choroid plexus was insensitive to serotonin. These results indicate that the serotonin recognition sites in the choroid plexus are not linked in a stimulatory or inhibitory way to an adenylate cyclase, in contrast with other 5-HT-1 receptor subtypes.
D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D001667:Binding, Competitive; D002540:Cerebral Cortex / Q000201:enzymology / Q000378:metabolism; D002831:Choroid Plexus / Q000201:enzymology / Q000378:metabolism*; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000096:biosynthesis; D004298:Dopamine / Q000494:pharmacology; D004873:Ergolines / Q000378:metabolism; D006624:Hippocampus / Q000201:enzymology / Q000378:metabolism; D006632:Histamine / Q000494:pharmacology; D007700:Kinetics; D008803:Mianserin / Q000378:metabolism; D051381:Rats; D011985:Receptors, Serotonin / Q000378:metabolism*; D012701:Serotonin / Q000378:metabolism / Q000494:pharmacology; D013552:Swine
D004873:Ergolines; D011985:Receptors, Serotonin; D005576:Colforsin; D008803:Mianserin; D012701:Serotonin; D006632:Histamine; D000242:Cyclic AMP; D000262:Adenylyl Cyclases; C031649:mesulergine; D004298:Dopamine