title: Changes in GABAergic system parameters after chronic administration of bicuculline.
abstract: Male Wistar rats were chronically treated with 2 mg/kg per day bicuculline i.p. during 10 days. Biochemical parameters related to the GABAergic system in CNS were analyzed. A 63% increase in glutamic acid decarboxylase activity was found in treated animals. Endogenous GABA levels were not modified. The treatment increased Bmax of GABA binding by 112% without changing the KD value. The treatment decreased Bmax of diazepam binding by 40% whereas it did not change the KD value. Diazepam binding was enhanced by in vitro addition of GABA at the same extent as in treated animals.
mesh_terms: D000818:Animals; D001640:Bicuculline / Q000008:administration & dosage / Q000494:pharmacology*; D001921:Brain / Q000378:metabolism*; D002531:Cerebellum / Q000378:metabolism; D002540:Cerebral Cortex / Q000201:enzymology / Q000378:metabolism; D003975:Diazepam / Q000378:metabolism; D005968:Glutamate Decarboxylase / Q000378:metabolism; D007274:Injections, Intraperitoneal; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D011963:Receptors, GABA-A / Q000187:drug effects; D013570:Synaptic Membranes / Q000378:metabolism; D005680:gamma-Aminobutyric Acid / Q000378:metabolism* / Q000502:physiology
chemical_list: D011963:Receptors, GABA-A; D005680:gamma-Aminobutyric Acid; D005968:Glutamate Decarboxylase; D003975:Diazepam; D001640:Bicuculline