Onset of neoplastic phenotype in an epithelial cell strain from adult BALB/c mouse lung alveolus.
Prolonged culture of NAL1A in 1 microM dexamethasone (CAS: 50-02-2) at low passage numbers resulted in the emergence of a morphologically altered and malignant cell strain, NAL1AM. (NAL1A was derived from lungs of normal adult female inbred BALB/c mice and exhibits several characteristics of epithelial cells.) A clone of NAL1A, B5, was shown to undergo a spontaneous morphologic alteration during culture in normal medium to resemble NAL1AM and cells of NUL1, a strain cultured directly from urethane (CAS: 51-79-6)-induced adenomas of mouse lung. Whereas NAL1A did not form colonies in soft agar, the clone B5 of NAL1A as well as NAL1AM and NUL1 showed quite high anchorage-independent growth (colony-forming efficiency, 6.3-7.8%). Compared with NAL1A cells, clone B5, NAL1AM, and NUL1 each exhibited a ninefold-reduced level of cellular binding of epidermal growth factor.
D000818:Animals; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D002478:Cells, Cultured; D003907:Dexamethasone / Q000494:pharmacology; D004848:Epithelium / Q000473:pathology; D066246:ErbB Receptors; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D010641:Phenotype; D011650:Pulmonary Alveoli / Q000473:pathology*; D011956:Receptors, Cell Surface / Q000032:analysis
D011956:Receptors, Cell Surface; D003907:Dexamethasone; D066246:ErbB Receptors