title: The influence of omeprazole on the synthesis and secretion of pepsinogen in isolated rabbit gastric glands.
abstract: Regulation mechanisms of pepsinogen (EC 3.4.23.) synthesis and secretion were studied by following newly synthesized [14C]-labeled pepsinogen during culture of isolated rabbit gastric glands. Omeprazole, a substituted benzimidazole, while almost completely abolishing acid production at 10(-4) M, strongly stimulated secretion of preformed and newly synthesized pepsinogen. Although the pepsinogen synthesis at this concentration of omeprazole was reduced to about 55% of the control rate, a two-fold absolute increase of total secreted pepsinogen was found. This increase was not due to a non specific leakage through disruption of chief cell membranes, as no increase of lactate dehydrogenase in the culture medium could be demonstrated. The stimulated secretion was influenced neither by 10(-3) M cimetidine, 10(-3) sodium thiocyanate nor 10(-4) M atropine. No additivity was found between the carbachol (10(-4) M) or dibutyryl cyclic AMP (10(-3) M) and the omeprazole induced pepsinogen secretion.
mesh_terms: D000818:Animals; D001562:Benzimidazoles / Q000494:pharmacology*; D003994:Bucladesine / Q000494:pharmacology; D002217:Carbachol / Q000494:pharmacology; D002250:Carbon Radioisotopes; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D005744:Gastric Acid / Q000378:metabolism; D005753:Gastric Mucosa / Q000187:drug effects* / Q000201:enzymology; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D009853:Omeprazole; D010435:Pepsinogens / Q000096:biosynthesis* / Q000378:metabolism; D011817:Rabbits
chemical_list: D001562:Benzimidazoles; D002250:Carbon Radioisotopes; D010435:Pepsinogens; D003994:Bucladesine; D002217:Carbachol; D007770:L-Lactate Dehydrogenase; D009853:Omeprazole