Inbred guinea pig aortic endothelial cell clones. Model for studying the vascular endothelium under totally isologous conditions.
Aortic endothelial cell clones were established from adult inbred strain 2 guinea pigs by using a simple method requiring neither feeder layers nor growth factors other than isologous serum. One clone has been maintained in continuous culture for 26 passages over a 9-month period. Cloned endothelial cells grew in a cobblestone pattern at confluence, formed intercellular junctions, and expressed factor-VIII-related antigen by immunofluorescence microscopy. The cloned cells also contained high levels of angiotensin-converting enzyme activity, although the level of enzyme activity expressed by different cloned cell lines varied. Cloned endothelial cells contained numerous Weibel-Palade bodies which appeared larger and less electron-dense than those of strain 2 guinea pig aortic endothelial cells in vivo. These clones may serve as an in vitro model to investigate the formation and function of this organelle. Cloned populations of strain 2 guinea pig vascular endothelial cells maintained in medium supplemented only with strain 2 guinea pig serum also provide an immunologically isologous system ideal for investigating interactions between the vascular endothelium and immunocompetent leukocytes in vitro.
D000818:Animals; D000941:Antigens / Q000032:analysis; D001013:Aorta, Thoracic; D001769:Blood; D002478:Cells, Cultured; D002999:Clone Cells / Q000166:cytology* / Q000201:enzymology / Q000276:immunology; D004727:Endothelium / Q000166:cytology* / Q000201:enzymology / Q000276:immunology; D005169:Factor VIII / Q000032:analysis / Q000276:immunology; D005353:Fibronectins / Q000032:analysis; D005455:Fluorescent Antibody Technique; D006168:Guinea Pigs; D008854:Microscopy, Electron; D007703:Peptidyl-Dipeptidase A / Q000378:metabolism; D013045:Species Specificity; D014841:von Willebrand Factor
D000941:Antigens; D005353:Fibronectins; D014841:von Willebrand Factor; D005169:Factor VIII; D007703:Peptidyl-Dipeptidase A