Expression of the c-Ha-ras and c-myc genes in aflatoxin B1-induced hepatocellular carcinomas.
Expression and activation of several c-oncogenes in seven hepatocellular carcinomas from seven separate rats treated with aflatoxin B1 (AFB1) were examined by Northern and Southern blot analyses. Both c-Ha-ras and c-myc transcripts were elevated at high levels in all hepatomas. Moreover, in one of them, T2-1 hepatoma, the c-myc gene was amplified only in a tumor part of liver without significant rearrangement. N-ras specific transcripts were not elevated in these hepatomas. The present data suggest that the consistently increased expression or deregulation of the c-myc and c-Ha-ras genes may play an important role in the development of hepatomas induced by AFB1.
D016604:Aflatoxin B1; D000348:Aflatoxins; D000818:Animals; D002273:Carcinogens; D002471:Cell Transformation, Neoplastic; D004262:DNA Restriction Enzymes; D005784:Gene Amplification; D008114:Liver Neoplasms, Experimental / Q000235:genetics*; D008297:Male; D009693:Nucleic Acid Hybridization; D009857:Oncogenes; D010957:Plasmids; D014176:Protein Biosynthesis; D051381:Rats; D011916:Rats, Inbred F344; D014158:Transcription, Genetic
D000348:Aflatoxins; D002273:Carcinogens; D016604:Aflatoxin B1; D004262:DNA Restriction Enzymes