title: Achlya mitochondrial DNA: gene localization and analysis of inverted repeats.
abstract: Mitochondrial DNA from four strains of the oomycete Achlya has been compared and nine gene loci mapped, including that of the ribosomal protein gene, var1. Examination of the restriction enzyme site maps showed the presence of four insertions relative to a map common to all four strains. All the insertions were found in close proximity to genic regions. The four strains also contained the inverted repeat first observed in A. ambisexualis (Hudspeth et al. 1983), allowing an examination by analysis of retained restriction sites of the evolutionary stability of repeated DNA sequences relative to single copy sequences. Although the inverted repeat is significantly more stable than single copy sequences, more detailed analysis indicates that this stability is limited to the portion encoding the ribosomal RNA genes. Thus, the apparent evolutionary stability of the repeat does not appear to derive from the inverted repeat structure per se.
mesh_terms: D001483:Base Sequence; D004262:DNA Restriction Enzymes; D004272:DNA, Mitochondrial / Q000235:genetics*; D005658:Fungi / Q000235:genetics*; D005800:Genes, Fungal; D009693:Nucleic Acid Hybridization; D013045:Species Specificity
chemical_list: D004272:DNA, Mitochondrial; D004262:DNA Restriction Enzymes