title: Nucleotide sequence of the gene determining plasmid-mediated citrate utilization.
abstract: The citrate utilization determinant from transposon Tn3411 has been cloned and sequenced, and its polypeptide products have been characterized in minicell experiments. The nucleotide sequence was determined for a 2,047-base-pair BglII restriction endonuclease fragment that includes the citrate determinant. This region contains an open reading frame that would encode a 431-amino-acid very hydrophobic polypeptide and which is preceded by a reasonable ribosomal binding site. However, the single polypeptide found in minicell experiments had an apparent molecular weight of 35,000 on sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002872:Chromosome Deletion; D002951:Citrates / Q000378:metabolism*; D019343:Citric Acid; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics; D005470:Fluorometry; D009690:Nucleic Acid Conformation; D010641:Phenotype; D010957:Plasmids
chemical_list: D002951:Citrates; D004251:DNA Transposable Elements; D019343:Citric Acid; D004262:DNA Restriction Enzymes