Formation of labelled colloid in 99Tcm-DMSA due to the presence of bactericidal fluid.
The possibility that traces of bactericidal fluid in 99Tcm-DMSA could lead to the formation of labelled colloid, was explored. In vitro investigations were undertaken using ultracentrifugation techniques and photon correlation spectroscopy. The latter showed that both contaminated and uncontaminated DMSA contained colloidal (or particulate) material. However the presence of 10 microliters bactericidal fluid as contaminant was shown by ultracentrifugation to result in labelling of this colloidal material when 99Tcm was added to DMSA. Studies in a normal volunteer confirmed the results of the in vitro studies, in that significant liver and spleen uptake was observed after the administration of contaminated 99Tcm-DMSA.
D000891:Anti-Infective Agents, Local / Q000494:pharmacology*; D004340:Drug Contamination; D006801:Humans; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism; D013670:Sodium Pertechnetate Tc 99m; D013057:Spectrum Analysis; D013154:Spleen / Q000378:metabolism; D004113:Succimer / Q000032:analysis* / Q000378:metabolism; D013438:Sulfhydryl Compounds / Q000032:analysis*; D013667:Technetium / Q000032:analysis* / Q000378:metabolism; D019783:Technetium Tc 99m Dimercaptosuccinic Acid; D013671:Technetium Tc 99m Sulfur Colloid / Q000032:analysis* / Q000378:metabolism
D000891:Anti-Infective Agents, Local; D013438:Sulfhydryl Compounds; D019783:Technetium Tc 99m Dimercaptosuccinic Acid; D013671:Technetium Tc 99m Sulfur Colloid; D013667:Technetium; D013670:Sodium Pertechnetate Tc 99m; D004113:Succimer