Immunochemical study on the mannans of Candida albicans NIH A-207, NIH B-792, and J-1012 strains prepared by fractional precipitation with cetyltrimethylammonium bromide.
The mannans of Candida albicans NIH A-207 (A strain, serotype A), C. albicans NIH B-792 (B strain, serotype B), and C. albicans J-1012 (J strain, serotype C) prepared by fractional precipitation with cetyltrimethylammonium bromide (Cetavlon) were investigated for their immunochemical properties. Upon treatment with 10 mM HCl at 100 degrees C for 60 min, the mannans of A and B strains each released a mixture of manno-oligosaccharides ranging from hexaose to mannose together with (for each one) an acid-modified mannan, while J-strain mannan released lower oligosaccharides, tetraose to mannose. The acid-modified mannan of B strain did not show antibody-precipitating activity against homologous antiserum, whereas acid-modified A- and J-strain mannans retained most of this activity. The acid-released oligosaccharides were assumed to consist of beta-1,2-linked D-mannopyranosyl residues from the results of specific rotation and proton magnetic resonance studies.
D000946:Antigens, Fungal / Q000302:isolation & purification*; D002176:Candida albicans / Q000032:analysis* / Q000276:immunology; D002241:Carbohydrates / Q000032:analysis; D000077286:Cetrimonium; D002593:Cetrimonium Compounds; D011232:Chemical Precipitation; D002850:Chromatography, Gel; D005656:Fungal Proteins / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D008351:Mannans / Q000276:immunology / Q000302:isolation & purification*; D010710:Phosphates / Q000302:isolation & purification; D011522:Protons; D000644:Quaternary Ammonium Compounds; D012441:Saccharomyces cerevisiae / Q000276:immunology
D000946:Antigens, Fungal; D002241:Carbohydrates; D002593:Cetrimonium Compounds; D005656:Fungal Proteins; D008351:Mannans; D010710:Phosphates; D011522:Protons; D000644:Quaternary Ammonium Compounds; D000077286:Cetrimonium