[Mutagenic effect of the SV40 oncogene: induction of resistance to 6-mercaptopurine and serum independence].
The mutagenic and transforming activity of SV40 DNA fragment, corresponding to its oncogene (the gene for large T antigen) was studied in Chinese hamster cells. After expression time of 3 to 4 days, the oncogene induced mutations of resistance to 6-mercaptopurine (6MP), while the DNA encoding the SV40 late genes, as well as DNA of Chinese hamster cells, were devoid of mutagenic activity. The value of induction ranged from 10(-4) to 10(-5). After the same expression time, the oncogene induced a typical character of oncogenic transformation - independence of serum growth factors (ser+). The value of induction of ser+ variants was somewhat higher than for resistance mutations. The study of 12 clones induced by the oncogene has shown the ser+ character to be hereditary, the expression of viral oncogene being not necessary for its maintenance. The data obtained support the hypothesis in favour of the participation of mutations of cellular genes in viral carcinogenesis.
D000818:Animals; D000957:Antigens, Viral, Tumor / Q000235:genetics*; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D004279:DNA, Viral / Q000235:genetics; D004351:Drug Resistance; D015122:Mercaptopurine / Q000494:pharmacology*; D009154:Mutation; D009857:Oncogenes; D013539:Simian virus 40 / Q000235:genetics* / Q000276:immunology; D014162:Transfection
D000957:Antigens, Viral, Tumor; D004279:DNA, Viral; D015122:Mercaptopurine
Mutagennoe deĭstvie onkogena SV40: induktsiia rezistentnosti k 6-merkaptopurinu i nezavisimost' ot syvorotki.