Spin-density distribution in the [FeO2]-complex. Electron spin resonance of myoglobin single crystals.
X-irradiated oxymyoglobin (MbO2) exhibits electron spin resonance (ESR) spectra at 77 K due to two distinct [FeO2]-centers formed by electron addition to the dioxygen. In single crystals with 17O and 57Fe isotope enrichment in the heme-ligand complex, the full set of spectral parameters is analyzed for one of the centers (gmax = 2.23, gint = 2.13, gmin = 1.97; HOmax = 2.66 mT, HOint = 1.61 mT, HOmin = 0.57 mT; HFe max = 1.62 mT, HFe int = 0.57 mT, HFe min = 0.49 mT) and the iron-dioxygen spin-density distribution and bonding geometry is derived. The g-tensor is evaluated for the second species at 77 K (gmax = 2.25, gint = 2.11, gmin = 1.95). Both centers transform into secondary species at 180 K for which the g-tensor elements are analyzed (gmax = 2.31, gint = 2.18, gmin = 1.93; gmax = 2.35, gint = 2.21, gmin = 1.91).
D003460:Crystallization; D004578:Electron Spin Resonance Spectroscopy; D006418:Heme / Q000032:analysis; D007501:Iron / Q000032:analysis; D009211:Myoglobin / Q000032:analysis*; D010100:Oxygen / Q000032:analysis
D009211:Myoglobin; D006418:Heme; D007501:Iron; D010100:Oxygen