Long-lasting blockade of P2-receptors of the urinary bladder in vivo following photolysis of arylazido aminopropionyl ATP, a photoaffinity label.
In the present study the possibility of provoking an irreversible blockade of P2-receptors in vivo by photolyzing ANAPP3 was investigated. ANAPP3 administered as an intraarterial infusion produced a short-lived blockade of P2-receptor mediated contractions of cat urinary bladders in situ. However, irradiation of the bladders with visible light during the infusion of ANAPP3 resulted in a blockade of the response for at least 120 minutes. Thus, photoactivation of ANAPP3 in vivo produced an essentially irreversible blockade of P2-purinergic receptors.
D000255:Adenosine Triphosphate / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000378:metabolism; D000345:Affinity Labels / Q000378:metabolism*; D000818:Animals; D001386:Azides / Q000008:administration & dosage / Q000378:metabolism*; D002417:Cattle; D005260:Female; D007261:Infusions, Intra-Arterial; D008027:Light; D008297:Male; D010782:Photolysis; D011687:Purines / Q000502:physiology*; D017981:Receptors, Neurotransmitter / Q000187:drug effects / Q000502:physiology*; D011983:Receptors, Purinergic; D001743:Urinary Bladder / Q000502:physiology*
C012761:3'-O-(3-(N-(4-azido-2-nitrophenyl)amino)propionyl)adenosine-5'-triphosphate; D000345:Affinity Labels; D001386:Azides; D011687:Purines; D017981:Receptors, Neurotransmitter; D011983:Receptors, Purinergic; D000255:Adenosine Triphosphate