[Detection of enzootic bovine leukemia virus using the syncytium test].
BLV detection by the syncytial test was performed in 27 heifers experimentally and naturally infected by the enzootic bovine leukosis virus (BLV). The presence of BLV was demonstrated in 94.7% of the animals. The bovine foetal spleen cells (FBS) were found to be suitable for the syncytial test. Positive animals not reacting to infection by the production of anti-BLV antibodies were identified during the syncytial-test investigation. The importance of this finding for the programme of controlling enzootic bovine leukosis on farms is discussed. As suggested by the results, temporary occurrence of anti-BLV antibodies followed by their disappearance can be observed together with a negative result of the syncytial test in some circumstances. The discussion deals with the problems of the determination of anti-BLV antibodies in milk, and/or milk secretion, by the ELISA method.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D003588:Cytopathogenic Effect, Viral; D001909:Leukemia Virus, Bovine / Q000276:immunology / Q000302:isolation & purification*; D007942:Leukemia, Experimental / Q000382:microbiology*; D008214:Lymphocytes / Q000382:microbiology; D012190:Retroviridae / Q000302:isolation & purification*
D000914:Antibodies, Viral
Prükaz viru enzootické leukózy skotu (BLV) syncytiálním testem (SyT).