Frequent generation of new 3'-defective interfering particles of vesicular stomatitis virus.
We have isolated and partially characterized a number of different genome types of defective interfering (DI) particles newly generated by a highly heat-resistant strain of vesicular stomatitis virus in either Rat(B77) or Vero cells. Northern blot analyses revealed that many of these DI genomes contain N gene sequences and/or sequences of the NS, M, and G genes. One type contains NS sequences without any indication for the presence of either N, M, or G sequences. Another type of DI particle genomes did not contain any detectable sequences of N, NS, M, or G, but contain panhandle-type sequences and, thus, most likely resembles the 5'-panhandle-type DI particles. Unlike previously assumed, these data demonstrate that DI genomes which have the 3'-terminal N, NS, M, and G genes or portions of these genes conserved do frequently arise together with 5'-DI particle genomes after serial undiluted passages of the heat-resistant strain of vesicular stomatitis virus.
D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D003673:Defective Viruses / Q000235:genetics*; D005814:Genes, Viral; D007668:Kidney; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification*; D051381:Rats; D014721:Vesicular stomatitis Indiana virus / Q000235:genetics*
D012367:RNA, Viral