Monoclonal antibody NCC-pX-1G reactive with gene products coded from X regions of human T-cell leukemia virus.
Monoclonal antibody NCC-pX-1G (IgG1, kappa) was obtained by the hybridoma technique by immunization of mice with the C-terminal 54 amino acids of the pX protein, made from the 3'-terminal portion of the pX gene conjugated with bovine growth hormone gene transfected in E. coli. NCC-pX-1G recognized 41 kilodalton pX protein of HTLV-I, and immunocytochemically stained HUT102 and other HTLV-I-infected cell lines, but no reaction was observed with non-HTLV-I-infected cell lines or normal human tissue cells.
D000818:Animals; D000911:Antibodies, Monoclonal; D002417:Cattle; D002460:Cell Line; D017977:Deltaretrovirus / Q000235:genetics*; D005819:Genetic Markers; D013006:Growth Hormone / Q000235:genetics; D006801:Humans; D006825:Hybridomas; D007120:Immunochemistry; D051379:Mice; D012191:Retroviridae Proteins / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics*
D000911:Antibodies, Monoclonal; D005819:Genetic Markers; D012191:Retroviridae Proteins; D014764:Viral Proteins; D013006:Growth Hormone