Antiidiotypic antibody to reovirus binds to neurons and protects from viral infection.
A syngeneic monoclonal antiidiotype directed against the idiotype of an antireovirus type 3 hemagglutinin demonstrates several of the biological actions of the original viral hemagglutinin and binds to rat and murine cortical neurons grown in dissociated cell culture. Receptor-bearing neurons appear within 24 hours of plating in cultures from mouse or rat cortex taken on embryonic day 15; these neurons are demonstrable for the duration of the culture life span (4 to 8 weeks). When cortical cultures are incubated with antiidiotype before or during exposure to reovirus, the antiidiotype protects neurons from type 3 infection without inhibiting infection of nonneuronal cells with either type 3 or type 1. Thus an antibody directed against a viral receptor can prevent infection of receptor-bearing cells without directly neutralizing the virus.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000627:therapeutic use*; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D006389:Hemagglutinins, Viral / Q000276:immunology; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D012089:Mammalian orthoreovirus 3 / Q000276:immunology; D051379:Mice; D051381:Rats; D011991:Receptors, Virus / Q000276:immunology*; D012088:Reoviridae Infections / Q000517:prevention & control*
D000911:Antibodies, Monoclonal; D006389:Hemagglutinins, Viral; D007130:Immunoglobulin Idiotypes; D011991:Receptors, Virus