The effect of formulation conditions on the distribution of 99mTc(NaBH4)-HEDP components separated by anion exchange HPLC.
Reduction of 99mTcO4- by NaBH4 in the presence of HEDP leads to a mixture of 99mTc-containing complexes which can be separated by anion exchange high performance liquid chromatography (HPLC). The distribution of complexes within this mixture can be varied by controlling pH, the concentration of HEDP, the concentration of technetium, and the presence of air. Suitable control of these formulation conditions can yield mixtures which consist of essentially (greater than 85%) one component. Arguments are presented to support the view that the components of 99mTc(NaBH4)-HEDP and 99mTc(NaBH4)-MDP mixtures are in fact oligomeric or polymeric complexes that can contain technetium centers in at least two different oxidation states.
D001842:Bone and Bones / Q000000981:diagnostic imaging; D001894:Borohydrides; D004338:Drug Combinations; D012968:Etidronic Acid; D006801:Humans; D011854:Radiochemistry; D011877:Radionuclide Imaging; D013667:Technetium / Q000302:isolation & purification*
D001894:Borohydrides; D004338:Drug Combinations; D013667:Technetium; C025364:sodium borohydride; D012968:Etidronic Acid