Human lymphocytes induce a differential release of prostaglandin E2 and interleukin 1-like mononuclear cell factor from normal blood monocytes.
Lymphocytes influence the production of both prostaglandin E2 (PGE2) and interleukin 1 (IL 1) by monocytes. We have examined whether these two products are released concomitantly or not under identical culture conditions using monocyte- and lymphocyte-enriched populations obtained on Percoll gradient. IL 1 was measured as mononuclear cell factor (IL 1/MCF). When incubated with concanavalin A, the monocyte-enriched fraction (MF; 80-91% monocytes), but not the lymphocyte-enriched fraction (LF; 95% lymphocytes) produced increasing amounts of PGE2 and MCF. However, when LF cells were added to MF cells in culture, a 40% to 60% decrease of PGE2 secretion was observed whereas the MCF production remained unchanged or increased up to 26-fold. Such a dissociation between IL 1 and PGE2 production by monocytes indicates independent regulation mechanisms in controlling the immune response under the influence of lymphocytes.
D002469:Cell Separation; D002499:Centrifugation, Density Gradient; D015232:Dinoprostone; D006801:Humans; D007375:Interleukin-1 / Q000096:biosynthesis*; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology*; D008262:Macrophage Activation; D009000:Monocytes / Q000378:metabolism*; D015846:Monokines; D011205:Povidone; D011458:Prostaglandins E / Q000378:metabolism*; D014176:Protein Biosynthesis; D012822:Silicon Dioxide
D007375:Interleukin-1; D015846:Monokines; D011458:Prostaglandins E; C016039:Percoll; D012822:Silicon Dioxide; D011205:Povidone; D015232:Dinoprostone