Cholesterol metabolism: use of D2O for determination of synthesis rate in cell culture.
Cholesterol synthesis in cell culture in the presence of D2O yields a spectrum of enriched molecules having a relative abundance that indicates random substitution of deuterium for hydrogen. Quantitation of the absolute rate of cholesterol synthesis is obtained by isotope ratio mass spectrometry. Mevinolin and 26-hydroxycholesterol both decrease cholesterol synthesis rate but have a discordant effect on HMG-CoA reductase activity.
D000818:Animals; D002478:Cells, Cultured; D002784:Cholesterol / Q000378:metabolism*; D006224:Cricetinae; D003412:Cricetulus; D003903:Deuterium; D017666:Deuterium Oxide; D005260:Female; D006888:Hydroxycholesterols / Q000494:pharmacology; D007700:Kinetics; D008074:Lipoproteins / Q000494:pharmacology; D008148:Lovastatin; D009281:Naphthalenes / Q000494:pharmacology; D014867:Water
D006888:Hydroxycholesterols; D008074:Lipoproteins; D009281:Naphthalenes; D014867:Water; C007042:cholest-5-ene-3 beta,26-diol; D002784:Cholesterol; D008148:Lovastatin; D003903:Deuterium; D017666:Deuterium Oxide