Preparation and characterization of F-protein vesicles isolated from Sendai virus by means of octyl glucoside.
We have demonstrated that Triton X-100 is always present in F-protein vesicles at concentrations that can provoke cell lysis. In order to avoid any misinterpretation of the fusogenic capacity of this protein, we solubilized the Sendai virus using octyl glucoside, which can be totally removed from the F protein preparation in less than 16 h by dialysis in the presence of absorbent beads. F-glycoprotein preparations preserved their ability to lyse erythrocytes in the presence of lectins and to induce cell-vesicle fusion as demonstrated by ESR studies. These vesicles were characterized by electron microscopy and SDS-polyacrylamide gel electrophoresis. Lipid analysis of these preparations by thin-layer chromatography indicated that they had the same proportion of lipids as virus envelopes, with slight variations in the sphingomyelin content and the cholesterol/phospholipid molar ratio. F-protein vesicles of different sizes can be obtained by adding exogenous lipids before detergent removal. The hemolytic activity of the vesicles was retained over a large range of lipid concentrations. We conclude that F-protein vesicles prepared with octyl glucoside are convenient tools for studying the fusogenic mechanism of this protein and improving the fusion process between liposomes and cells.
D000818:Animals; D002642:Chick Embryo; D004578:Electron Spin Resonance Spectroscopy; D004591:Electrophoresis, Polyacrylamide Gel; D005960:Glucosides; D006023:Glycoproteins / Q000302:isolation & purification*; D006461:Hemolysis; D006801:Humans; D007700:Kinetics; D008854:Microscopy, Electron; D008970:Molecular Weight; D010222:Parainfluenza Virus 1, Human / Q000032:analysis*; D014759:Viral Envelope Proteins / Q000302:isolation & purification*; D014760:Viral Fusion Proteins
D005960:Glucosides; D006023:Glycoproteins; D014759:Viral Envelope Proteins; D014760:Viral Fusion Proteins; C018619:octyl-beta-D-glucoside