Cloning and expression of the Escherichia coli rho gene in a plasmid vector.
In order to further elucidate the role of Rho protein on transcription termination and cells growth control, we have subcloned by two steps the rho+ structural gene of Escherichia coli from Lambda rho+524 into a plasmid vector. The resulting plasmid pEG25 contains a 2.9 kbp insert which is able to complement several different rho mutations and to express a functional Rho protein in U.V. irradiated maxicells.
D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004926:Escherichia coli / Q000235:genetics* / Q000528:radiation effects; D005796:Genes; D005798:Genes, Bacterial; D005822:Genetic Vectors; D005838:Genotype; D008970:Molecular Weight; D009154:Mutation; D010957:Plasmids; D012234:Rho Factor / Q000235:genetics* / Q000302:isolation & purification; D013045:Species Specificity; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic; D014466:Ultraviolet Rays
D012234:Rho Factor; D014157:Transcription Factors; D004262:DNA Restriction Enzymes