Separation of human endothelial cells from fibroblasts by centrifugation in Percoll gradients.
Human endothelial cells from the umbilical vein and skin fibroblasts can be separated by means of centrifugation in a density gradient of Percoll. Cells show a good recovery in culture. Viability is not impaired.
D002469:Cell Separation / Q000379:methods*; D002499:Centrifugation, Density Gradient; D004727:Endothelium / Q000166:cytology; D005347:Fibroblasts / Q000166:cytology*; D006801:Humans; D011205:Povidone; D012822:Silicon Dioxide; D014471:Umbilical Veins / Q000166:cytology*
C016039:Percoll; D012822:Silicon Dioxide; D011205:Povidone