Truncated forms of the polyomavirus middle T antigen can substitute for the small T antigen in lytic infection.
Cloned polyomavirus genomes encoding the small T antigen or truncated forms of the middle T antigen facilitated the growth of genomes encoding only the large T antigen in mouse 3T6 cells. We conclude that an N-terminal domain of the middle T antigen, in the appropriate cellular location, can substitute for the small T antigen during lytic infection.
D000818:Animals; D000952:Antigens, Polyomavirus Transforming; D000957:Antigens, Viral, Tumor / Q000235:genetics / Q000502:physiology*; D002451:Cell Compartmentation; D002460:Cell Line; D002472:Cell Transformation, Viral; D003001:Cloning, Molecular; D003588:Cytopathogenic Effect, Viral; D004279:DNA, Viral / Q000235:genetics; D051379:Mice; D009856:Oncogene Proteins, Viral / Q000235:genetics / Q000502:physiology*; D010446:Peptide Fragments / Q000235:genetics / Q000502:physiology*; D011120:Polyomavirus / Q000235:genetics / Q000502:physiology*; D011994:Recombinant Proteins / Q000502:physiology; D014162:Transfection
D000952:Antigens, Polyomavirus Transforming; D000957:Antigens, Viral, Tumor; D004279:DNA, Viral; D009856:Oncogene Proteins, Viral; D010446:Peptide Fragments; D011994:Recombinant Proteins