Effects of S-adenosylhomocysteine and analogs on Epstein-Barr virus-induced transformation, expression of the Epstein-Barr virus capsid antigen, and methylation of Epstein-Barr virus DNA.
S-Adenosylhomocysteine was found to have no effect on Epstein-Barr virus-induced transformation of B-lymphocytes and to stimulate viral capsid antigen expression only slightly in the FF41-1 cell line. In contrast, the S-adenosylhomocysteine analogs sinefungin and S-isobutyladenosine inhibited Epstein-Barr virus transformation and induced a significant increase in the numbers of cells expressing the viral capsid antigen. An inverse relationship between levels of viral DNA methylation and gene expression was demonstrated.
D002213:Capsid / Q000235:genetics*; D002460:Cell Line; D002472:Cell Transformation, Viral / Q000187:drug effects*; D004279:DNA, Viral / Q000235:genetics / Q000378:metabolism*; D004854:Herpesvirus 4, Human / Q000187:drug effects / Q000235:genetics*; D006710:Homocysteine / Q000031:analogs & derivatives*; D006801:Humans; D008214:Lymphocytes / Q000166:cytology / Q000382:microbiology; D008745:Methylation; D012435:S-Adenosylhomocysteine / Q000031:analogs & derivatives* / Q000494:pharmacology*; D013329:Structure-Activity Relationship
D004279:DNA, Viral; D006710:Homocysteine; D012435:S-Adenosylhomocysteine