Large-molecular-weight thymocyte-activating factors in extracellular matrix of SV40-transformed human embryo fibroblasts.
It has been reported that the major thymocyte-activating factors at 10-15 kDa exist in the culture medium of SV40-transformed human embryo fibroblasts. However, these factors could not be detected in the extracellular matrix (ECM) from the same transformed cells, but the 30-40 kDa factors were found. They showed a similar lectin requirement, charge heterogeneity and Concanavalin A-binding property as compared with the factors reported previously. They were separated from fibroblast DNA synthetic activities in ECM and did not contain interleukin 2 activity. A possible relationship between 30-40 kDa factors in ECM and 10-15 kDa factors in the culture medium is discussed.
D001688:Biological Products / Q000302:isolation & purification*; D002460:Cell Line; D002472:Cell Transformation, Viral; D016207:Cytokines; D004622:Embryo, Mammalian; D005109:Extracellular Matrix / Q000276:immunology*; D005347:Fibroblasts; D006801:Humans; D008213:Lymphocyte Activation; D008970:Molecular Weight; D013539:Simian virus 40; D013601:T-Lymphocytes / Q000276:immunology*
D001688:Biological Products; D016207:Cytokines; C051922:thymocyte-activating factor, fibroblasts