Role of ner protein in bacteriophage Mu transposition.
The Ner protein of bacteriophage Mu negatively regulates transcription initiated at the early promoter and at the major repressor promoter. The construction and isolation of a Ner- mutant of Mu is described. Ner is an essential function for Mu, because the mutant phage only forms plaques when complemented for Ner. Mutations in the repressor protein did not abolish the need for Ner. However, when transcription of the repressor gene c was blocked by deleting the major repressor promoter, Ner was no longer essential for normal Mu development.
D010583:Bacteriophage mu / Q000235:genetics*; D004261:DNA Replication; D004251:DNA Transposable Elements; D005814:Genes, Viral; D009154:Mutation; D011401:Promoter Regions, Genetic; D012097:Repressor Proteins / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000235:genetics; D014779:Virus Replication
D004251:DNA Transposable Elements; D012097:Repressor Proteins; D014157:Transcription Factors; D014764:Viral Proteins