Quantitation, with a new assay, of Theiler's virus capsid protein in the central nervous system of mice.
We developed a quantitative assay for antigens at the single-cell level. Tissue sections were reacted with a primary antibody, a biotinylated secondary antibody, or 35S-streptavidin. Binding of streptavidin to cells was quantitated by microscopic autoradiography. We showed that the number of autoradiographic grains was proportional to the amount of antigen per cell. With this assay, we studied the synthesis of Theiler's virus capsid proteins VP1, VP2, and VP3 in permissive BHK cells grown in vitro and in mouse central nervous system (CNS) cells during a persistent infection. We found that synthesis of the three capsid proteins was restricted in mouse CNS cells. Restricted virus replication could play a major role in the persistence of Theiler's virus in mouse CNS cells.
D000818:Animals; D001345:Autoradiography; D002213:Capsid / Q000032:analysis / Q000096:biosynthesis*; D002460:Cell Line; D006224:Cricetinae; D007158:Immunologic Techniques; D051379:Mice; D010849:Picornaviridae / Q000378:metabolism*; D010850:Picornaviridae Infections / Q000382:microbiology*; D013116:Spinal Cord / Q000382:microbiology*; D013118:Spinal Cord Diseases / Q000382:microbiology*; D014764:Viral Proteins / Q000032:analysis / Q000096:biosynthesis*; D015678:Viral Structural Proteins
D014764:Viral Proteins; D015678:Viral Structural Proteins