Bidirectional SV40 transcription mediated by tandem Sp1 binding interactions.
The 21-base pair repeat elements of the SV40 promoter contain six tandem copies of the GGGCGG hexanucleotide (GC-box), each of which can bind, with varying affinity, to the cellular transcription factor, Sp1. In vitro SV40 early RNA synthesis is mediated by interaction of Sp1 with GC-boxes I, II, and III, whereas transcription in the late direction is mediated by binding to GC-boxes III, V, and VI.
D001345:Autoradiography; D001483:Base Sequence; D001665:Binding Sites; D004268:DNA-Binding Proteins / Q000378:metabolism*; D003850:Deoxyribonuclease I / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005786:Gene Expression Regulation; D009154:Mutation; D011257:Pregnancy Proteins / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D012367:RNA, Viral / Q000096:biosynthesis; D013539:Simian virus 40 / Q000235:genetics*; D016329:Sp1 Transcription Factor; D013698:Templates, Genetic; D014157:Transcription Factors / Q000378:metabolism*; D014158:Transcription, Genetic
D004268:DNA-Binding Proteins; D011257:Pregnancy Proteins; D012333:RNA, Messenger; D012367:RNA, Viral; D016329:Sp1 Transcription Factor; D014157:Transcription Factors; D003850:Deoxyribonuclease I