Corynebacterium parvum-elicited hepatic macrophages demonstrate enhanced respiratory burst activity compared with resident Kupffer cells in the rat.
We have recently demonstrated that release of oxygen-derived free radicals by activated hepatic macrophages may be involved in the pathogenesis of a rat model of liver injury induced by Corynebacterium parvum and endotoxin. In the present study we have compared the respiratory burst activity of isolated normal rat Kupffer cells with that of hepatic macrophages elicited by C. parvum. Superoxide production (O2-.) and glucose oxidation via the hexose monophosphate shunt (HMPS) were low in normal Kupffer cells, but were significantly increased (O2-. 2.1-fold, HMPS 1.7-fold) by phorbol myristate acetate, a stimulant of the respiratory burst. Corynebacterium parvum-elicited hepatic macrophages demonstrated significantly enhanced superoxide production and HMPS activity compared with normal Kupffer cells, both in the absence of specific stimuli (O2-. 3.3-fold, HMPS 5.3-fold) and after exposure to phorbol myristate acetate (O2-. 4.5-fold, HMPS 5.3-fold). These results demonstrate that normal Kupffer cells are capable of exhibiting respiratory burst activity, but this is markedly increased for hepatic macrophages elicited by an inflammatory stimulus.
D000818:Animals; D003354:Corynebacterium Infections / Q000378:metabolism*; D005609:Free Radicals / Q000378:metabolism; D007728:Kupffer Cells / Q000378:metabolism*; D008107:Liver Diseases / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism; D008297:Male; D010427:Pentose Phosphate Pathway; D051381:Rats; D011919:Rats, Inbred Strains; D012119:Respiration; D013481:Superoxides / Q000378:metabolism
D005609:Free Radicals; D013481:Superoxides