Direct regional assignment of the gene for vitamin D binding protein (Gc-globulin) to human chromosome 4q11-q13 and identification of an associated DNA polymorphism.
Using a characterized human vitamin D binding protein (DBP) cDNA probe and a panel of rodent X human somatic cell hybrids, we established the chromosomal location of the structural gene for DBP on human chromosome 4. In situ hybridization of 3H-labeled DBP cDNA to human metaphase chromosomes confirmed this assignment and allowed regional localization to bands 4q11-4q13. A restriction fragment length polymorphism associated with the DBP gene that should prove useful in future linkage studies was identified with the enzyme BamHI.
D000483:Alleles; D000818:Animals; D002871:Chromosome Banding; D002874:Chromosome Mapping; D002905:Chromosomes, Human, 4-5; D006224:Cricetinae; D004247:DNA / Q000235:genetics; D004262:DNA Restriction Enzymes; D015245:Deoxyribonuclease BamHI; D005796:Genes; D008040:Genetic Linkage; D006801:Humans; D006822:Hybrid Cells; D007621:Karyotyping; D011110:Polymorphism, Genetic; D014809:Vitamin D-Binding Protein / Q000235:genetics*
D014809:Vitamin D-Binding Protein; D004247:DNA; D004262:DNA Restriction Enzymes; D015245:Deoxyribonuclease BamHI