Antigen presentation by liposomes: inhibition with antibodies.
The involvement of both antigen and class II major histocompatibility complex (MHC) molecules in T cell activation by liposome-bound antigens was investigated. We used a pigeon cytochrome c (PCC)-specific Ek-restricted T cell hybridoma that can be activated to produce interleukin 2 by liposomes carrying either PCC and Ek molecules, or a high concentration of PCC alone. We demonstrated that the MHC-restricted response of this hybridoma to liposomes is specifically blocked by both anti-MHC and anti-PCC monoclonal antibodies, whereas unrestricted activation is only inhibited by PCC-specific antibody.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000502:physiology*; D000938:Antigen-Presenting Cells / Q000276:immunology*; D001667:Binding, Competitive; D002999:Clone Cells / Q000276:immunology; D010856:Columbidae; D003574:Cytochrome c Group / Q000276:immunology; D005260:Female; D006649:Histocompatibility Antigens / Q000276:immunology; D000949:Histocompatibility Antigens Class II; D006825:Hybridomas / Q000276:immunology; D008081:Liposomes / Q000276:immunology*; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D013601:T-Lymphocytes / Q000276:immunology
D000911:Antibodies, Monoclonal; D003574:Cytochrome c Group; D006649:Histocompatibility Antigens; D000949:Histocompatibility Antigens Class II; C024138:I-E-antigen; D008081:Liposomes