Collagenase synthesis in clonal rabbit odontoblast-like cells (RP cells).
Post-confluent cultures of cloned rabbit odontoblast-like cells (RP: Rabbit Pulp cells) produced latent collagenase, which was isolated from serum-containing culture media by heparin-Sepharose affinity chromatography. RP collagenase was purified 4,420-fold from serum-containing medium to a specific activity of 1,313 units/mg with a yield of 14% by heparin-Sepharose affinity chromatography, carboxymethyl-cellulose ion-exchange chromatography, and by immunoadsorption chromatography. The RP cell culture appears to be a suitable model to use for studying collagenase synthesis in mineralized tissue-forming cells and for elucidating the mechanism of collagen degradation in pulp tissue and dentin matrix.
D000818:Animals; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D002999:Clone Cells; D003470:Culture Media / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D007163:Immunosorbent Techniques; D003012:Microbial Collagenase / Q000096:biosynthesis* / Q000302:isolation & purification; D009804:Odontoblasts / Q000166:cytology / Q000201:enzymology*; D011817:Rabbits
D003470:Culture Media; D003012:Microbial Collagenase