[Use of an RNAse inhibitor from the human placenta in specific fragmentation of high molecular weight RNA by ribonuclease H].
Human placenta RNase inhibitor has been shown to suppress the interfering ribonucleases, virtually not affecting the RNase H activity. This allows the usage of the inhibitor in the course of site-specific cleavage of high molecular weight RNAs by ribonuclease H.
D001435:Bacteriophages / Q000032:analysis / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D004722:Endoribonucleases / Q000494:pharmacology*; D005260:Female; D006801:Humans; D006868:Hydrolysis; D066298:In Vitro Techniques; D010920:Placenta / Q000201:enzymology*; D011247:Pregnancy; D012367:RNA, Viral / Q000032:analysis*; D016914:Ribonuclease H; D012260:Ribonucleases / Q000037:antagonists & inhibitors*
D012367:RNA, Viral; D004722:Endoribonucleases; D012260:Ribonucleases; D016914:Ribonuclease H
Ispol'zovanie inhibittora RNKaz iz platsenty cheloveka pri spetsificheskoĭ fragmentatsii vysokomolekuliarnykh RNK ribonukleazoĭ H.