Functional receptors for VIP, GIP, glucagon-29 and -37 in the HGT-1 human gastric cancer cell line.
Three separate sets of receptors sensitive to VIP, GIP and pancreatic/entero-glucagons, have been characterized in HGT-1 cells. The order of relative potencies of VIP receptor agonists was VIP greater than rh GRF-43, rh GRF-29 greater than PHI greater than hp GRF-40, secretin. G-37 was about 4 times less potent than G-29 in HGT-1 cells (G-29 greater than G-37), whereas it was about 20 times more potent than G-29 in rat fundic glands (G-37 greater than G-29). Adenylate cyclase in HGT-1 cells was stimulated by VIP, G-29, G-37 and GIP, over a concentration from 3.16 X 10(-9) to 3.16 X 10(-7) M GIP. The experimental data: (1) support the enterogastrone activity of GIP, via adenylate cyclase activation and somatostatin release by gastric D cells; (2) demonstrate that HGT-1 cells originating from a human fundic tumor are sensitive to the glucagon-like peptides G-29 and -37, as rat fundic glands; (3) indicate that the pharmacological properties of the VIP receptor in this human gastric cell line are similar to those characterized in normal human gastric glands.
D000262:Adenylyl Cyclases / Q000378:metabolism; D002460:Cell Line; D000242:Cyclic AMP / Q000378:metabolism; D005749:Gastric Inhibitory Polypeptide / Q000378:metabolism; D005934:Glucagon / Q000378:metabolism; D004763:Glucagon-Like Peptides / Q000378:metabolism; D006801:Humans; D011956:Receptors, Cell Surface / Q000378:metabolism*; D011964:Receptors, Gastrointestinal Hormone; D018027:Receptors, Glucagon; D018005:Receptors, Vasoactive Intestinal Peptide; D013274:Stomach Neoplasms / Q000378:metabolism*; D014660:Vasoactive Intestinal Peptide / Q000378:metabolism
D011956:Receptors, Cell Surface; D011964:Receptors, Gastrointestinal Hormone; D018027:Receptors, Glucagon; D018005:Receptors, Vasoactive Intestinal Peptide; D014660:Vasoactive Intestinal Peptide; D005749:Gastric Inhibitory Polypeptide; D004763:Glucagon-Like Peptides; D005934:Glucagon; C041086:gastric inhibitory polypeptide receptor; D000242:Cyclic AMP; D000262:Adenylyl Cyclases