The abnormal location of cytoplasmic SV40 large T is not caused by failure to bind to DNA or to p53.
We have examined the large T encoded by an SV40 mutant, d10, which fails to localize to the nucleus. The DNA sequence of the mutant predicts the alteration of Lys 128----Thr within the sequence 127 Lys Lys Lys Arg Lys 131 of large T. The results show that d10 large T is capable of binding to SV40 DNA, to cellular DNA and to the cellular phosphoprotein p53 as well as wild-type large T. These data suggest that the cytoplasmic location of d10 large T is not due to an inability of the protein to be retained within the nucleus, but argues instead that the protein fails to reach the nucleus because it contains a defective nuclear location signal.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D000952:Antigens, Polyomavirus Transforming; D000957:Antigens, Viral, Tumor / Q000235:genetics* / Q000378:metabolism; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D004262:DNA Restriction Enzymes; D051379:Mice; D009363:Neoplasm Proteins / Q000378:metabolism*; D009698:Nucleoproteins / Q000378:metabolism*; D009856:Oncogene Proteins, Viral / Q000235:genetics* / Q000378:metabolism; D010750:Phosphoproteins / Q000378:metabolism*; D010761:Phosphorus Radioisotopes; D010957:Plasmids; D011485:Protein Binding; D011494:Protein Kinases / Q000378:metabolism*; D013539:Simian virus 40 / Q000235:genetics*; D016159:Tumor Suppressor Protein p53
D000952:Antigens, Polyomavirus Transforming; D000957:Antigens, Viral, Tumor; D009363:Neoplasm Proteins; D009698:Nucleoproteins; D009856:Oncogene Proteins, Viral; D010750:Phosphoproteins; D010761:Phosphorus Radioisotopes; D016159:Tumor Suppressor Protein p53; D000255:Adenosine Triphosphate; D011494:Protein Kinases; D004262:DNA Restriction Enzymes