title: Construction of cloning, promoter-screening, and terminator-screening shuttle vectors for Bacillus subtilis and Streptococcus lactis.
abstract: Shuttle vectors have been constructed which are suitable both for the selection of regulatory sequences and for gene cloning in Bacillus subtilis and Streptococcus lactis. The promoter screening vectors contain a promoterless chloramphenicol acetyltransferase gene; the insertion of suitable DNA fragments upstream of the gene restored the enzyme activity. With a related set of vectors, transcription termination signals can be selected.
mesh_terms: D000123:Acetyltransferases / Q000235:genetics; D001412:Bacillus subtilis / Q000235:genetics*; D015500:Chloramphenicol O-Acetyltransferase; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D005798:Genes, Bacterial; D005809:Genes, Regulator; D005822:Genetic Vectors; D013294:Lactococcus lactis / Q000235:genetics*; D011401:Promoter Regions, Genetic; D013728:Terminator Regions, Genetic
chemical_list: D000123:Acetyltransferases; D015500:Chloramphenicol O-Acetyltransferase; D004262:DNA Restriction Enzymes