title: Characterization of macrophages elicited by intraperitoneal injection of hyaluronate.
abstract: Hyaluronate of 120,000 molecular weight has been injected in the peritoneal cavity of mice to study its effect on migration of inflammatory cells in vivo. After one day a dose-dependent granulocyte migration is observed. Three days later the number of granulocytes is greatly reduced and macrophages form about half of the total cell population. Hyaluronate-elicited macrophages show a decreased 5'-nucleotidase and an increased acid phosphatase activity as compared to resident macrophages. The production of superoxide anion in response to the phorbol ester tetradecanoyl-phorbolacetate, and the phagocytic activity are also enhanced. Macrophages elicited by hyaluronate secrete growth factor(s) for non-lymphoid mesenchymal cells. It is concluded that hyaluronate in vivo stimulates the migration of inflammatory cells, thus causing the recruitment of a population of stimulating macrophages. These effects may explain previous reports on the acceleration of wound healing by hyaluronate.
mesh_terms: D015720:5'-Nucleotidase; D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D002455:Cell Division; D006820:Hyaluronic Acid / Q000008:administration & dosage / Q000494:pharmacology*; D007274:Injections, Intraperitoneal; D007700:Kinetics; D008264:Macrophages / Q000166:cytology* / Q000201:enzymology / Q000502:physiology; D008297:Male; D051379:Mice; D009708:Nucleotidases / Q000378:metabolism; D010587:Phagocytosis
chemical_list: D006820:Hyaluronic Acid; D009708:Nucleotidases; D000135:Acid Phosphatase; D015720:5'-Nucleotidase