Further investigation on the mode of entry of human rotavirus into cells.
Entry of the KUN strain of human rotavirus into MA 104 cells was studied by electron microscopy. Double-shelled rotavirus particles attached to the cell membrane, and in the presence of trypsin their nucleic acids were expelled from the virus core into the cytoplasm through radial spaces between the capsomeres and the cell membrane pores formed after their attachment. This mechanism was considered to be analogous to those of phages.
D001692:Biological Transport; D002478:Cells, Cultured; D004492:Edetic Acid / Q000494:pharmacology; D006358:Hot Temperature; D006801:Humans; D008854:Microscopy, Electron; D012367:RNA, Viral / Q000378:metabolism*; D012401:Rotavirus / Q000502:physiology*; D014357:Trypsin / Q000378:metabolism; D014779:Virus Replication
D012367:RNA, Viral; D004492:Edetic Acid; D014357:Trypsin