Compensatory mutations suggest that base-pairing with a small nuclear RNA is required to form the 3' end of H3 messenger RNA.
Processing of the 3' end of sea urchin H3 histone pre-mRNA requires conserved sequence elements and the presence of U7 snRNA. A mutation in the conserved CAAGAAGA sequence of the H3 pre-mRNA that renders 3' processing of this precursor defective is shown to be suppressed by a compensatory change in the U7 snRNA, restoring the base-pairing potential of the two RNAs. RNA-RNA contacts between these two molecules appear to be an essential feature of the 3' processing reaction.
D000818:Animals; D001483:Base Sequence; D004262:DNA Restriction Enzymes / Q000378:metabolism; D006657:Histones / Q000235:genetics; D009154:Mutation; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D009694:Nucleic Acid Precursors / Q000032:analysis; D009865:Oocytes / Q000032:analysis; D012322:RNA Precursors; D012333:RNA, Messenger / Q000032:analysis*; D012342:RNA, Small Nuclear / Q000032:analysis*; D012617:Sea Urchins; D014981:Xenopus
D006657:Histones; D009694:Nucleic Acid Precursors; D012322:RNA Precursors; D012333:RNA, Messenger; D012342:RNA, Small Nuclear; D004262:DNA Restriction Enzymes