Suppression of in vitro human antithyroglobulin antibody secretion by private and cross-reactive anti-idiotypic antibodies.
This is a report on suppression of in vitro human antithyroglobulin antibody secretion in Epstein-Barr (EB) virus-transformed B lymphocytes by private and cross-reactive anti-idiotypic antibodies. Two polyclonal anti-idiotypic antibodies, private anti-Yo-Id and cross-reactive anti-Uc-Id antibodies, were raised in rabbits. Human IgG, lambda antithyroglobulin antibody-producing cell line (Yo3) and human IgG-producing cell line (Yo5), which did not contain the antithyroglobulin activity were established by EB virus transformation of peripheral lymphocytes of patient Yo with chronic thyroiditis. Binding of anti-Yo-Id and anti-Uc-Id antibodies to IgG F(ab')2 antithyroglobulin derived from patient Yo was significantly inhibited by human thyroglobulin and affinity-purified IgG antithyroglobulin of Yo3 cell line. Both private anti-Yo-Id and cross-reactive anti-Uc-Id antibodies suppressed the antithyroglobulin antibody secretion in Yo3 cell line. But these anti-idiotypic antibodies did not suppress the IgG secretion in Yo5 cell line. These results suggested that interactions between idiotype and anti-idiotype may play a role in the immune system of human chronic thyroiditis.
D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D000918:Antibody Specificity; D001323:Autoantibodies / Q000276:immunology*; D002460:Cell Line; D002472:Cell Transformation, Viral; D003429:Cross Reactions; D004854:Herpesvirus 4, Human; D006801:Humans; D007108:Immune Tolerance; D007140:Immunoglobulin Fab Fragments / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D013954:Thyroglobulin / Q000276:immunology*; D013967:Thyroiditis, Autoimmune / Q000276:immunology*
D000888:Antibodies, Anti-Idiotypic; D001323:Autoantibodies; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D007130:Immunoglobulin Idiotypes; D013954:Thyroglobulin