ELISA for detection of IgG and IgM antibodies to HSV-1 and HSV-2 in human sera.
A rapid, enzyme-linked immunoassay (ELISA) was applied to identify and measure specific IgG and IgM antibodies to herpes simplex viruses types 1 and 2 (HSV-1 and HSV-2). Detergent solubilized infected cells and mock-infected cells were used as antigens in the assay. Identification of type-specific antibodies was achieved by a competition assay in which clinical sera mixed with HSV-1 or HSV-2 antigens were assayed for reactivity to identical antigens coating wells of polystyrene microtiter plates. Reactivity and the specificity of the reactive immunoglobulin class was quantitated using biotinylated goat anti-IgG and biotinylated goat anti-IgM. Five paired sera from patients with diagnosed herpes simplex genital infections and one human anti-HSV-1 reference serum were tested with this assay and results were compared to results previously obtained using a complement fixation test and micro-SPRIA. The results indicate that the ELISA is a specific, sensitive and simple test which confirms the herpes simplex virus infection history of patients.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D003168:Complement Fixation Tests; D004797:Enzyme-Linked Immunosorbent Assay; D006558:Herpes Genitalis / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis*; D007075:Immunoglobulin M / Q000032:analysis*; D011863:Radioimmunoassay; D018139:Simplexvirus / Q000276:immunology*; D014709:Vero Cells
D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M