Monoclonal antibodies to cytochrome c from Paracoccus denitrificans: effects on electron transport reactions.
The effect of a monoclonal antibody to a soluble cytochrome c from Paracoccus denitrificans was tested on the membrane-bound electron-transport system of this bacterium. This antibody (F3-10.2) and one previously described (F3-29.4) (Kuo, L.M., Davies, H.C. and Smith, L. (1984) Biochim. Biophys. Acta 766, 472-482) were deduced to bind to the cytochrome c in the area including amino acid residue number 23 on a loop on the side of the heme crevice. In contrast to the observations with the previously tested antibody, the present data show the second antibody to block completely the reaction of the cytochrome c with cytochrome c oxidase but not that with cytochrome c reductase. Neither antibody has an appreciable inhibitory effect on the NADH oxidase of the isolated detergent-treated membranes. The two antibodies bind in different ways, giving insight into the interaction of a soluble protein with membrane-bound enzymes. The data indicate that the reaction sites on the cytochrome c for the oxidase and reductase moieties of P. denitrificans are different. They also argue against the need for a dissociable cytochrome c comparable to that which functions on the mitochondrial inner membrane.
D000911:Antibodies, Monoclonal; D001666:Binding Sites, Antibody; D003574:Cytochrome c Group / Q000276:immunology* / Q000378:metabolism; D004579:Electron Transport; D003576:Electron Transport Complex IV / Q000378:metabolism; D009245:NADH Dehydrogenase / Q000378:metabolism; D010231:Paracoccus denitrificans / Q000201:enzymology*; D011048:Polarography; D012995:Solubility; D013053:Spectrophotometry
D000911:Antibodies, Monoclonal; D003574:Cytochrome c Group; D009245:NADH Dehydrogenase; D003576:Electron Transport Complex IV