CRF immunoreactive peptides in the human hypophysis: a cautionary note.
By monitoring with a non competitive enzyme linked immunosorbent assay (ELISA), corticotropin releasing factor (CRF)-like immunoreactive material was isolated from the human hypophysis. After acid extraction of peptides from frozen human hypophyses, the purification was achieved by affinity chromatography using purified anti-ovine-CRF IgG bound to a solid phase and then by two HPLC steps using an alkylsilane-bonded large pore size silica. Two CRF-like peptides were purified: discrete immunoreactive peaks coinciding with an optical density peak at 215 nm. Although these peptides were recognized by ELISA, they were not recognized in an RIA using the same anti-ovine-CRF serum and ovine CRF-41 as tracer. Neither of these CRF-immunoreactive peptides had any effect on either the spontaneous or stimulated ACTH release in the perfused isolated anterior pituitary cell bioassay.
D000324:Adrenocorticotropic Hormone / Q000378:metabolism; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D003346:Corticotropin-Releasing Hormone / Q000032:analysis* / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007164:Immunosorbents; D010902:Pituitary Gland / Q000032:analysis*; D010903:Pituitary Gland, Anterior / Q000187:drug effects / Q000378:metabolism; D011863:Radioimmunoassay / Q000379:methods
D000596:Amino Acids; D007164:Immunosorbents; D000324:Adrenocorticotropic Hormone; D003346:Corticotropin-Releasing Hormone