Nuclease recognition of an alternating structure in a d(AT)14 plasmid insert.
The nuclease reactivity and specificity of a cloned tract of poly X (dA-dT) X poly(dA-dT) has been explored. Digestion with DNAse I, Mung Bean nuclease, S1 nuclease, DNAse II, and copper (1,10-phenanthroline)2 on a 256 base pair restriction fragment containing d(AT)14A revealed a dinucleotide repeat structure for the alternating sequence. Furthermore, conditions which wind or unwind the linear DNA had little effect on the reactivity of the AT insert. These preferred cleavages offer insights to structural alterations within the DNA helix which differ from A, B, or Z-DNA. Nucleation into flanking sequences by this structural alteration was not observed.
D003850:Deoxyribonuclease I / Q000378:metabolism; D003851:Deoxyribonucleases / Q000378:metabolism*; D004706:Endodeoxyribonucleases / Q000378:metabolism; D004720:Endonucleases / Q000378:metabolism; D009690:Nucleic Acid Conformation; D010618:Phenanthrolines; D010957:Plasmids; D011067:Poly dA-dT / Q000378:metabolism; D011089:Polydeoxyribonucleotides / Q000378:metabolism*; D015719:Single-Strand Specific DNA and RNA Endonucleases; D013379:Substrate Specificity
D010618:Phenanthrolines; D011089:Polydeoxyribonucleotides; D011067:Poly dA-dT; D003851:Deoxyribonucleases; D004706:Endodeoxyribonucleases; D004720:Endonucleases; D003850:Deoxyribonuclease I; C019329:deoxyribonuclease II; D015719:Single-Strand Specific DNA and RNA Endonucleases; C025205:1,10-phenanthroline