Selection and characterization of an interferon-responsive clonal cell line of HeLa cells.
HeLa cells generally do not respond well to interferon (IFN). We have used is-1, an IFN-sensitive mutant of mengovirus, to select a clone of IFN-responsive HeLa cells (F-H12). At moderate levels of human alpha/beta IFN, is-1 yields were fivefold lower in these cells than in similarly protected control cells. In contrast, wild-type mengovirus, vesicular stomatitis virus and a wild-type and thymidine kinase-negative strains of herpes simplex virus type 1 grew equally well in both cell lines. By a cell survival assay, the F-H12 line was up to 100 times more responsive to IFN than the parental line when challenged by is-1. 2'-5'-Oligo(A)-dependent endonuclease activity was the same in both lines. These observations cannot be accounted for by enhanced induction of IFN following infection.
D002470:Cell Survival; D002999:Clone Cells / Q000187:drug effects; D004722:Endoribonucleases / Q000378:metabolism; D006367:HeLa Cells / Q000187:drug effects*; D006801:Humans; D007370:Interferon Type I / Q000494:pharmacology*; D008574:Mengovirus / Q000502:physiology; D018139:Simplexvirus / Q000502:physiology; D014721:Vesicular stomatitis Indiana virus / Q000502:physiology; D014779:Virus Replication / Q000187:drug effects
D007370:Interferon Type I; D004722:Endoribonucleases; C031956:2-5A-dependent ribonuclease