Stimulation by dolichol phosphate-mannose of N-acetylglucosaminyl-lipid biosynthesis by membranes from class E Thy-1-negative mutant mouse lymphoma cells which are defective in dolichol phosphate-mannose biosynthesis.
Dolichol phosphate-mannose has been shown previously to stimulate the biosynthesis of N-acetylglucosaminyl-diphosphate-dolichol (E. L. Kean (1985) J. Biol. Chem. 260, 12561-12571). Although the class E Thy-1-negative mutant mouse lymphoma cells are unable to synthesize dolichol phosphate-mannose, the addition of this compound exogenously to membranes from the mutant cells brought about a stimulation of N-acetylglucosaminyl-lipid synthesis similar to that obtained with membranes from wild type cells. The retention of this activity by the mutant cells supports the suggestion of a regulatory role for dolichol phosphate-mannose as an intrinsic property of the glucosaminyltransferase which catalyzes the initial reaction of the dolichol pathway.
D000117:Acetylglucosamine / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D004287:Dolichol Monophosphate Mannose / Q000096:biosynthesis / Q000494:pharmacology*; D005944:Glucosamine / Q000031:analogs & derivatives*; D006017:Glycolipids / Q000096:biosynthesis*; D008223:Lymphoma / Q000378:metabolism; D008563:Membrane Lipids / Q000096:biosynthesis*; D051379:Mice; D009154:Mutation; D011105:Polyisoprenyl Phosphate Sugars / Q000494:pharmacology*; D011755:Pyrophosphatases / Q000037:antagonists & inhibitors
D006017:Glycolipids; D008563:Membrane Lipids; D011105:Polyisoprenyl Phosphate Sugars; D004287:Dolichol Monophosphate Mannose; D011755:Pyrophosphatases; D005944:Glucosamine; D000117:Acetylglucosamine