Some basic properties of sheep liver cAMP-dependent protein kinase.
Sheep liver cytosol (105,000 X g supernatant) yields two major peaks of protein kinase by using DEAE-Trisacryl M as an ion-exchange resin at pH 7.0. Peak I (Type-I), corresponding to 30-50% of the total activity, is not retained by the column at a starting ionic strength of ca. 0.06 M, while Peak-II (Type-II) is eluting at 0.17 M ionic strength. Both peaks are found to be dependent on cAMP and are active on histone (ATP: Protein phosphotransferase, EC 2.7.1.37). Kms apparents for histone and ATP are 1.5 +/- 0.5 mg/ml and 16 +/- 4 microM, respectively, for PrK-I while that of PrK-II are 1.8 mg/ml and 28.6 microM, respectively. Both enzymes are found to be stable for two weeks at 4 degrees C. Molecular weight determination of crude extract (105,000 X g supernatant) show three peaks corresponding to the molecular weights of 251,000; 131,800 and 43,600.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002852:Chromatography, Ion Exchange; D000242:Cyclic AMP / Q000378:metabolism; D006657:Histones / Q000378:metabolism; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008970:Molecular Weight; D011494:Protein Kinases / Q000302:isolation & purification / Q000378:metabolism*; D012756:Sheep / Q000378:metabolism*
D006657:Histones; D000255:Adenosine Triphosphate; D000242:Cyclic AMP; D011494:Protein Kinases