title: Self-limitation of the oxidative burst of rat polymorphonuclear leukocytes.
abstract: The oxidative response of rat polymorphonuclear leukocytes stimulated by phorbol myristate acetate and N-formyl-methionyl-leucyl-phenylalanine was studied. Ferricytochrome reduction and peroxidase-catalyzed decrease of scopoletin fluorescence were used to monitor O-2 and H2O2 release in the extracellular medium. Oxygen consumption was also measured in some experiments. Decrease of chlortetracyclin fluorescence after stimulation of dye-loaded cells was used to study an early step of cell stimulation. Finally, a possible relationship between cell responses and the medium redox potential was explored. Three major conclusions were obtained: Ferricytochrome reduction is dependent on the total cytochrome concentration, and a simple mathematical model allows a tentative estimate of total superoxide anion production by stimulated cells. Increasing cell concentration results in a decrease of individual cell response, and this may be accounted for by a direct inhibition of cell-released hydrogen peroxide on the reactivity of leukocytes. Further, H2O2 may be shown to inhibit an early step of cell response. The solution redox potential does not influence cell reactivity, since it may be dramatically decreased without inhibiting cell response.
mesh_terms: D000818:Animals; D002374:Catalase / Q000378:metabolism; D002751:Chlortetracycline; D003580:Cytochromes / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D010084:Oxidation-Reduction; D010101:Oxygen Consumption; D051381:Rats; D011919:Rats, Inbred Strains; D013481:Superoxides / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D003580:Cytochromes; D013481:Superoxides; D009240:N-Formylmethionine Leucyl-Phenylalanine; D006861:Hydrogen Peroxide; D002374:Catalase; D013755:Tetradecanoylphorbol Acetate; D002751:Chlortetracycline