Isolation and characterization of Cu,Zn superoxide dismutase of the shark Prionace glauca.
A Cu,Zn superoxide dismutase was purified for the first time from an elasmobranch species (Prionace glauca) and showed the following differences with respect to other animal superoxide dismutases. The enzyme displays a low isoelectric point. The enzyme activity is unusually independent of ionic strength. The isolated enzyme has 30% of its copper in the reduced state.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D003300:Copper / Q000032:analysis; D004578:Electron Spin Resonance Spectroscopy; D004591:Electrophoresis, Polyacrylamide Gel; D007525:Isoelectric Focusing; D007700:Kinetics; D008970:Molecular Weight; D009994:Osmolar Concentration; D010084:Oxidation-Reduction; D012754:Sharks / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000302:isolation & purification* / Q000378:metabolism
D000596:Amino Acids; D003300:Copper; D013482:Superoxide Dismutase