Amplification and rearrangement of Ki-ras oncogene in human teratocarcinoma-derived cell lines.
The structure of the ras gene family was analyzed in two human teratocarcinoma-derived cell lines, Tera I and Tera II, by DNA restriction enzyme digestion and Southern blot. We report here a ten-fold amplification of the c-Ki-ras-2 gene in these cell lines, whereas no structural alterations seem to occur either in c-Ha-ras-1 or N-ras. We also provide evidence indicating that no point mutation at codon 12, specifically recognized by Sac I, was detected. Moreover, DNA rearrangement, due to the loss of a Pvu II site located in the intervening sequences between the third and the fourth exon, has been found in both Tera I and Tera II.
D002460:Cell Line; D004262:DNA Restriction Enzymes; D005784:Gene Amplification; D006801:Humans; D009693:Nucleic Acid Hybridization; D009857:Oncogenes; D013724:Teratoma / Q000235:genetics*
D004262:DNA Restriction Enzymes