Establishment and characterization of a new human cloned myelomonocytic cell line (ZC-1.6).
A human cell line, ZC, derived from the blood of a patient with acute myelomonocytic leukemia, was established and cloned. One of the clones, ZC-1.6, was subsequently characterized. As for its morphology and cytochemistry, ZC-1.6 clone shares a number of features with immature cells of the monocytic or myelocytic lineages. Surface marker analysis shows positivity for 4F2 (100% of the cells), and OKM1 (38%) monoclonal antibodies, and presence of surface HLA-D/DR antigens (100%) and Fc (11%) and complement (C3b) receptors (100%). Functional capabilities of ZC-1.6 cells include adherence, spreading, and phagocytosis of latex and opsonized zymosan particles. Despite its morphological immaturity, the ZC-1.6 clone produces relevant amounts of O2- in the presence of different stimuli (phorbol myristate acetate, opsonized zymosan, or latex particles). The production of O2- by ZC-1.6 cells is the first evidence that reactive oxygen intermediate production may represent an early feature of cells of the myelomonocytic lineage.
D000328:Adult; D000838:Anions; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000032:analysis; D002448:Cell Adhesion; D002455:Cell Division; D002460:Cell Line; D002999:Clone Cells; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D006651:Histocytochemistry; D006801:Humans; D007621:Karyotyping; D007700:Kinetics; D015470:Leukemia, Myeloid, Acute / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D008297:Male; D008854:Microscopy, Electron; D010587:Phagocytosis; D011951:Receptors, Complement / Q000032:analysis; D011961:Receptors, Fc / Q000032:analysis; D012397:Rosette Formation; D013481:Superoxides / Q000378:metabolism
D000838:Anions; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D000949:Histocompatibility Antigens Class II; D011951:Receptors, Complement; D011961:Receptors, Fc; D013481:Superoxides