Superoxide photogeneration by chlorophyll a in water/acetone. Electron spin resonance studies of radical intermediates in chlorophyll a photoreaction in vitro.
In this paper we report the use of DMPO (5,5-dimethyl-1-pyrroline-1-oxide) as spin trap in the ESR observation of O2-. photogeneration by in vitro Ch1 a in oxygen-saturated 50:50% (v/v) water/acetone. The observed hyperfine parameters for the spin adducts of DMPO are identical to those obtained from H2O2 decomposition, and to those reported by earlier workers for the formation of 02-. in oxygen-saturated preparations of spinach chloroplasts.
D000096:Acetone; D002734:Chlorophyll / Q000378:metabolism*; D000077194:Chlorophyll A; D003497:Cyclic N-Oxides; D004578:Electron Spin Resonance Spectroscopy; D005609:Free Radicals; D010777:Photochemistry; D013481:Superoxides / Q000378:metabolism*; D014867:Water
D003497:Cyclic N-Oxides; D005609:Free Radicals; D014867:Water; D013481:Superoxides; D000096:Acetone; D002734:Chlorophyll; C017245:5,5-dimethyl-1-pyrroline-1-oxide; D000077194:Chlorophyll A