Disaggregation-induced changes of developmentally regulated proteins in Dictyostelium discoideum.
By means of two-dimensional gel electrophoresis, we analyzed proteins present in a slug-shaped tissue mass of D. discoideum and examined the changes in their amounts after disaggregation of the slugs. Of approximately one hundred polypeptides, six were found to decrease in amount after disaggregation. The decreases of four polypeptides were inhibited by the presence of 1 mM cAMP or 250 micrograms/ml cycloheximide. The decreases of the two other proteins were not suppressed by cAMP or cycloheximide. The patterns of proteins present in vegetative and aggregative cells were also examined. None of the six proteins which showed a decrease after slug disaggregation was found in vegetative or preaggregative cells. These results indicate that both synthesis and degradation of these proteins are controlled by cell-cell contact.
D002449:Cell Aggregation; D000242:Cyclic AMP / Q000494:pharmacology; D003513:Cycloheximide / Q000494:pharmacology; D004023:Dictyostelium / Q000166:cytology* / Q000378:metabolism; D005656:Fungal Proteins / Q000378:metabolism*; D007526:Isoelectric Point; D008970:Molecular Weight
D005656:Fungal Proteins; D003513:Cycloheximide; D000242:Cyclic AMP