In vitro attachment of mono- and oligosaccharides to surface glycoconjugates of intact cells.
We have synthesized glycosylhydrazines of various mono- and oligosaccharides and coupled these to periodate- or galactose oxidase-treated human red cells and K562 erythroleukemia cells. The optimal conditions for this carbohydrate modification of cells have been established. This method makes it possible to specifically elongate oligosaccharide chains of cell surface glycoconjugates with desired carbohydrates. In this way, new antigenic and receptor properties can be conferred to cells, and the functional roles of carbohydrates in cell surface glycoconjugates can be studied. The method has been used to make red cells of blood group O reactive with anti-A and anti-B sera, and in rendering K562 cells or red cells of blood group O agglutinable with the alpha-N-acetylgalactosamine-specific Helix pomatia lectin.
D000017:ABO Blood-Group System / Q000276:immunology; D000954:Antigens, Surface / Q000276:immunology; D002460:Cell Line; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D005692:Galactose Oxidase / Q000494:pharmacology; D006017:Glycolipids / Q000097:blood*; D006023:Glycoproteins / Q000097:blood*; D006801:Humans; D006834:Hydrazines / Q000097:blood; D004915:Leukemia, Erythroblastic, Acute / Q000378:metabolism; D008358:Mannose / Q000031:analogs & derivatives / Q000097:blood; D009005:Monosaccharides / Q000097:blood*; D009844:Oligosaccharides / Q000097:blood*; D010504:Periodic Acid / Q000494:pharmacology; D014168:Transferrin / Q000097:blood
D000017:ABO Blood-Group System; D000954:Antigens, Surface; D006017:Glycolipids; D006023:Glycoproteins; D006834:Hydrazines; D009005:Monosaccharides; D009844:Oligosaccharides; D014168:Transferrin; D010504:Periodic Acid; C048830:mannosylhydrazine; C009288:metaperiodate; D005692:Galactose Oxidase; D008358:Mannose