Autoantibodies to Alzheimer and normal brain structures from virus-transformed lymphocytes.
B-Lymphocytes from two patients with Alzheimer's disease and one healthy subject were transformed into lymphoblastoid cells by exposure to Epstein-Barr virus. In culture, more than 50% of these cells secreted sufficient IgM or IgG antibody (mainly IgM) to allow immunohistochemical screening against cryostat sections of normal and Alzheimer temporal cortex. More than 30% of the IgM antibodies from each subject recognised brain components, namely: neurons, astrocytes, nuclei, nucleoli, and Alzheimer plaques and neurofibrillary tangles. This methodology represents a major addition to the procedures currently available for the generation of antibodies towards normal and pathological structures in human brain.
D000544:Alzheimer Disease / Q000276:immunology* / Q000473:pathology; D000918:Antibody Specificity; D001253:Astrocytes / Q000276:immunology; D001323:Autoantibodies / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology*; D002467:Cell Nucleus / Q000276:immunology; D002472:Cell Transformation, Viral; D002540:Cerebral Cortex / Q000276:immunology*; D004854:Herpesvirus 4, Human; D006801:Humans; D007382:Intermediate Filaments / Q000276:immunology; D009474:Neurons / Q000276:immunology; D013702:Temporal Lobe
D001323:Autoantibodies