title: Novel structure of a human U6 snRNA pseudogene.
abstract: A genomic DNA library containing human placental DNA cloned into phage lambda Charon 4A was screened for snRNA U6 genes. In vitro 32P-labeled U6 snRNA isolated from HeLa cells was used as a hybridization probe. A positive clone containing a 4.6-kb EcoRI fragment of human chromosomal DNA was recloned into the EcoRI site of pBR325 and mapped by restriction endonuclease digestion. Restriction fragments containing U6 RNA sequences were identified by hybridization with isolated U6[32P]RNA. The sequence analysis revealed a novel structure of a U6 RNA pseudogene, bearing two 17-nucleotide(nt)-long direct repeats of genuine U6 RNA sequences arranged in a head-to-tail fashion within the 5' part of the molecule. Hypothetical models as to how this type of snRNA U6 pseudogene might have been generated during evolution of the human genome are presented. When compared to mammalian U6 RNA sequences the pseudogene accounts for a 77% overall sequence homology and contains the authentic 5'- and 3'-ends of the U6 RNA.
mesh_terms: D010582:Bacteriophage lambda / Q000235:genetics; D001483:Base Sequence; D005075:Biological Evolution; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism; D004262:DNA Restriction Enzymes; D005260:Female; D005796:Genes; D006801:Humans; D008957:Models, Genetic; D010920:Placenta / Q000378:metabolism; D010957:Plasmids; D011247:Pregnancy; D012342:RNA, Small Nuclear / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D012342:RNA, Small Nuclear; D004247:DNA; D004262:DNA Restriction Enzymes