title: Role of the radB gene in postreplication repair in UV-irradiated Escherichia coli uvrB.
abstract: In UV-irradiated Escherichia coli, the radB101 mutation sensitized uvrB recF cells 4-fold and uvrB recB cells 1.2-fold, but did not sensitize uvrB recB recF cells. The radB mutation had very little effect (1.2-fold or less) on the repair of UV radiation-induced DNA daughter-strand gaps in uvrB cells, but it did cause about a 3-fold deficiency in the repair of the DNA double-strand breaks that arise in association with nonrepaired daughter-strand gaps in UV-irradiated uvrB recF cells. Thus, the radB gene does not appear to be involved in the recF-dependent or recF recB-independent processes for the repair of DNA daughter-strand gaps, but is involved in the recB-dependent postreplication repair of DNA double-strand breaks.
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology; D002499:Centrifugation, Density Gradient; D004260:DNA Repair; D004269:DNA, Bacterial / Q000032:analysis / Q000528:radiation effects; D004706:Endodeoxyribonucleases / Q000235:genetics / Q000502:physiology; D004926:Escherichia coli / Q000235:genetics / Q000502:physiology* / Q000528:radiation effects; D029968:Escherichia coli Proteins; D043211:Exodeoxyribonuclease V; D005090:Exodeoxyribonucleases / Q000235:genetics / Q000502:physiology; D005798:Genes, Bacterial; D014466:Ultraviolet Rays
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D004706:Endodeoxyribonucleases; D005090:Exodeoxyribonucleases; D043211:Exodeoxyribonuclease V; C468322:exodeoxyribonuclease V, E coli; C036385:endodeoxyribonuclease uvrABC