title: Localization of cytochrome C oxidase and cytochrome C peroxidase in mitochondria of Hymenolepis diminuta (Cestoda).
abstract: The intramitochondrial localization of cytochrome c oxidase and cytochrome c peroxidase in adult Hymenolepis diminuta was investigated. Mitochondria were fractionated into inner membrane, outer membrane, intermembrane space and matrix and the efficacy of fractionation was monitored employing marker enzymes. Cytochrome c oxidase was associated with the mitochondrial inner membrane. Whereas 55% of the cytochrome c peroxidase activity was in the matrix, 32% of the activity was in the intermembrane space fraction. Based upon the distribution of marker enzymes, a dual compartmentalization of cytochrome c peroxidase is apparent in H. diminuta mitochondria.
mesh_terms: D000818:Animals; D002451:Cell Compartmentation; D003578:Cytochrome-c Peroxidase / Q000032:analysis*; D003576:Electron Transport Complex IV / Q000032:analysis*; D006926:Hymenolepis / Q000201:enzymology*; D007425:Intracellular Membranes / Q000201:enzymology; D008297:Male; D008928:Mitochondria / Q000201:enzymology*; D010544:Peroxidases / Q000032:analysis*; D051381:Rats
chemical_list: D010544:Peroxidases; D003578:Cytochrome-c Peroxidase; D003576:Electron Transport Complex IV