title: Genomic organization of the simian virus 40-adenovirus 7 hybrid virus, PARA(cT), that encodes a nuclear transport defective simian virus 40 T antigen.
abstract: The genomic organization of the simian virus 40 (SV40)-adenovirus (Ad)7 hybrid virus, PARA(cT), was examined. A deletion of approximately 5529 bp of Ad7 DNA extends from 78.8 map units to 94.0 map units and is replaced by an SV40 DNA insert of 3809 bp. The left-hand end of the insertion begins at SV40 nucleotide 5168, 5 bp upstream of the ATG initiation codon for T-ag synthesis. The sequence extends counterclockwise through the T-ag encoding sequences and into SV40 late region DNA. Most of the late region DNA has been removed in a deletion between nucleotides 2464 and 301. One of the 72-bp repeats has also been deleted. The right-hand end of the SV40 DNA insert is at nucleotide 4366. Thus, a portion of the SV40 DNA early region is repeated at both ends of the insert (nucleotides 5168-4366).
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D000957:Antigens, Viral, Tumor / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D001692:Biological Transport; D002467:Cell Nucleus / Q000378:metabolism; D002874:Chromosome Mapping; D004279:DNA, Viral / Q000235:genetics; D009154:Mutation; D011995:Recombination, Genetic; D013539:Simian virus 40 / Q000235:genetics*; D014760:Viral Fusion Proteins / Q000235:genetics
chemical_list: D000957:Antigens, Viral, Tumor; D004279:DNA, Viral; D014760:Viral Fusion Proteins