title: A rapid and efficient method to remove labelled molecular probes from nucleic acids immobilized on solid supports.
abstract: A rapid and efficient method is described for the removal of radio-active molecular probes from nucleic acids immobilized on nylon membranes. This method involves boiling in distilled water in a microwave oven. This procedure can be completed within ten minutes, does not require the use of any buffers or reagents, and produces results comparable with conventional buffer-wash procedures recommended by the suppliers of the transfer membranes.
mesh_terms: D000255:Adenosine Triphosphate; D001345:Autoradiography; D004247:DNA / Q000302:isolation & purification*; D004262:DNA Restriction Enzymes; D015247:Deoxyribonuclease HindIII; D007202:Indicators and Reagents; D009693:Nucleic Acid Hybridization; D009757:Nylons; D010761:Phosphorus Radioisotopes; D010957:Plasmids
chemical_list: D007202:Indicators and Reagents; D009757:Nylons; D010761:Phosphorus Radioisotopes; D000255:Adenosine Triphosphate; D004247:DNA; D004262:DNA Restriction Enzymes; D015247:Deoxyribonuclease HindIII