title: Synthesis and purification of 2-deoxy-2-[18F]fluoro-D-glucose and 2-deoxy-2-[18F]fluoro-D-mannose: characterization of products by 1H- and 19F-NMR spectroscopy.
abstract: A procedure has been developed that allows the separation of 2-deoxy-2-[18F]fluoro-D-glucose from 2-deoxy-2-[18F]fluoro-D-mannose employing selectively optimized ion-moderated partition chromatography. Both compounds can be obtained with a greater than 98% chemical and radiochemical purity in about one half-life of 18F. Both the alpha- and beta-anomers of both sugars were completely characterized by high-resolution 1H- and 19F-NMR spectroscopy. Various convenient preparation methods for 2-deoxy-2-[18F]fluoro-D-glucose were compared.
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003837:Deoxy Sugars / Q000138:chemical synthesis*; D003847:Deoxyglucose / Q000031:analogs & derivatives / Q000138:chemical synthesis* / Q000302:isolation & purification; D005461:Fluorine; D019788:Fluorodeoxyglucose F18; D009682:Magnetic Resonance Spectroscopy; D011868:Radioisotopes; D012210:Rhamnose / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000302:isolation & purification
chemical_list: D003837:Deoxy Sugars; D011868:Radioisotopes; D019788:Fluorodeoxyglucose F18; D005461:Fluorine; C017190:2-deoxy-2-fluoromannose; D003847:Deoxyglucose; D012210:Rhamnose