title: [Binding of tritiated imipramine to human platelets. 1 or several binding sites?].
abstract: Using Langers' method (10) under their laboratory conditions, the authors determined the characteristics of the high-affinity imipramine-binding site on human platelets in thirty-five normal subjects, equally divided between the sexes and distributed by decade from twenty to sixty years of age and above. Bmax was 440 +/- 121 fmol./mg. protein, Kd 0.96 +/- 0.62, 10(-9) M. Protein concentration was held within a range of 0.2 to 0.55 mg./ml. in order to avoid nonlinear bias with Bmax, expressed in fmol./ml. of incubate. The characteristics of the site did not co-vary with sex or age in this experimental series. However, subject to additional investigation, the curve of the Scatchard graph would seem to indicate the possible existence of a distinct, low-affinity imipramine-binding site.
mesh_terms: D000328:Adult; D000367:Age Factors; D001792:Blood Platelets / Q000378:metabolism*; D002352:Carrier Proteins; D005260:Female; D006801:Humans; D007099:Imipramine / Q000097:blood*; D008297:Male; D008875:Middle Aged; D011955:Receptors, Drug; D017981:Receptors, Neurotransmitter / Q000378:metabolism*; D012737:Sex Factors
chemical_list: D002352:Carrier Proteins; D011955:Receptors, Drug; D017981:Receptors, Neurotransmitter; C027775:imipramine receptor; D007099:Imipramine
vernacular_title: Liaison de l'imipramine tritiée sur les plaquettes humaines. Un ou plusieurs sites de liaison?