The enhancement of diazepam and muscimol binding by pentobarbital and (+)-etomidate: size of the molecular arrangement estimated by electron irradiation inactivation of rat cortex.
Synaptosomal membranes were prepared from frozen rat cortices irradiated by 10 MeV electrons and the enhancement of [3H]diazepam and [3H]muscimol binding by pentobarbital (PB) and (+)-etomidate was studied. The target sizes of the corresponding parts of the receptor complex were estimated from the decrease in the enhancement as a function of irradiation dose. Different radiation inactivation constants suggest different regulatory units for the enhancement by PB and (+)-etomidate. Target sizes for PB and (+)-etomidate enhancement of [3H]diazepam binding were 127 +/- 14 and 360 + 124 kDa, respectively.
D000818:Animals; D002540:Cerebral Cortex / Q000187:drug effects*; D003975:Diazepam / Q000378:metabolism*; D005045:Etomidate / Q000494:pharmacology*; D066298:In Vitro Techniques; D008970:Molecular Weight; D009118:Muscimol / Q000378:metabolism*; D010424:Pentobarbital / Q000494:pharmacology*; D051381:Rats; D011963:Receptors, GABA-A / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
D011963:Receptors, GABA-A; D009118:Muscimol; D005680:gamma-Aminobutyric Acid; D010424:Pentobarbital; D003975:Diazepam; D005045:Etomidate