Reduced temperature can block different glycoproteins at different steps during transport to the plasma membrane.
Reduced temperature has been shown to block the cell surface expression of Sendai virus haemagglutinin-neuraminidase (HN) and fusion (F0) glycoproteins at different steps of their intracellular transport. At 20 degrees C, HN was confined to the rough endoplasmic reticulum or cis Golgi compartment, while F0 acquired complete resistance to digestion by endo-beta-N-acetylglucosaminidase-H and therefore was blocked at a more distal location in the pathway of cell surface expression. The significance of these results for different pathways of transport to the cell surface is discussed.
D000818:Animals; D001692:Biological Transport; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism*; D006224:Cricetinae; D004721:Endoplasmic Reticulum / Q000378:metabolism; D006056:Golgi Apparatus / Q000378:metabolism; D015707:HN Protein; D010222:Parainfluenza Virus 1, Human / Q000378:metabolism*; D013696:Temperature; D014759:Viral Envelope Proteins / Q000378:metabolism*; D014760:Viral Fusion Proteins
D015707:HN Protein; D014759:Viral Envelope Proteins; D014760:Viral Fusion Proteins