Stable transformation of Drosophila Kc cells to antibiotic resistance with the bacterial neomycin resistance gene.
By transfection with a plasmid containing the APH(3') gene under control of the HSV I thymidine kinase promoter, independent series of stably transformed Drosophila cells were established and grown for more than one and a half years under highly selective pressure (2 mg G 418/ml). Analysis of transformed Drosophila cell DNAs shows that the APH(3') gene was integrated into the genome. Neomycin phosphotransferase is constitutively expressed in transformed cells. This efficient selective system by a dominant marker makes it possible to introduce, by cotransfection, any DNA sequence of interest into the genome of cultured Drosophila cells.
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001483:Base Sequence; D002460:Cell Line; D004247:DNA; D004262:DNA Restriction Enzymes; D004330:Drosophila / Q000235:genetics*; D004351:Drug Resistance; D004352:Drug Resistance, Microbial; D005839:Gentamicins / Q000494:pharmacology*; D019868:Kanamycin Kinase; D009355:Neomycin / Q000494:pharmacology*; D009693:Nucleic Acid Hybridization; D010770:Phosphotransferases / Q000235:genetics* / Q000378:metabolism; D010957:Plasmids; D014162:Transfection
D000900:Anti-Bacterial Agents; D005839:Gentamicins; D004247:DNA; C010680:antibiotic G 418; D010770:Phosphotransferases; D019868:Kanamycin Kinase; D004262:DNA Restriction Enzymes; D009355:Neomycin