title: Complementary DNA derived structure of the amino-terminal domain of human apolipoprotein B and size of its messenger RNA transcript.
abstract: In this paper the sequence of a 5.2-kilobase (kb) cDNA covering the amino-terminal domain of human apolipoprotein B-100 (apoB-100) is reported. The cDNA-derived protein sequence provides the primary structure of 1748 amino acids. This segment of apoB-100 is more hydrophilic than hydrophobic and contains short stretches of predicted helical and beta structures that are interrupted by beta turns. Blotting analysis of RNA isolated from fetal human and adult monkey tissues and various human cell lines showed synthesis of apoB mRNA by liver and intestine and by cells of hepatic (HepG2) and intestinal (Caco-2) origin. The isolation and characterization of overlapping cDNA clones, which provide a nearly full-length copy of human apoB-100, are also reported. From the length of these clones the size of the cytoplasmic apoB mRNA is estimated to be 14.0 kb and codes for a protein of approximately 512,000 daltons.
mesh_terms: D000328:Adult; D000595:Amino Acid Sequence; D001055:Apolipoproteins B / Q000235:genetics*; D001483:Base Sequence; D004247:DNA / Q000032:analysis*; D004262:DNA Restriction Enzymes; D005796:Genes; D006801:Humans; D008099:Liver / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D001055:Apolipoproteins B; D012333:RNA, Messenger; D004247:DNA; D004262:DNA Restriction Enzymes