Separable function of platelet release reaction and clot retraction.
Amiloride, a known Na+/H+ exchange inhibitor, inhibited platelet serotonin release in a dose-dependent manner (100 microM for 50% inhibition, and 1mM for the nearly complete inhibition), although amiloride (1mM) accelerated clot retraction when it was measured at decreased platelet concentration. On the contrary, cytochalasin B (10 micrograms/ml) accelerated platelet serotonin release, but it inhibited clot retraction. These results demonstrate that release reaction and clot retraction, both of which are important processes involved in platelet activation, can be functionally separated.
D000199:Actins / Q000378:metabolism; D000244:Adenosine Diphosphate / Q000494:pharmacology; D000584:Amiloride / Q000494:pharmacology; D001241:Aspirin / Q000494:pharmacology; D001792:Blood Platelets / Q000187:drug effects / Q000502:physiology*; D003018:Clot Retraction; D003571:Cytochalasin B / Q000494:pharmacology; D006801:Humans; D011522:Protons; D012701:Serotonin / Q000378:metabolism*; D012964:Sodium / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D000199:Actins; D011522:Protons; D012701:Serotonin; D003571:Cytochalasin B; D000244:Adenosine Diphosphate; D000584:Amiloride; D012964:Sodium; D013755:Tetradecanoylphorbol Acetate; D001241:Aspirin