Stimulation of Na,K-ATPase activity of frog skeletal muscle by insulin.
Na,K-ATPase activity of a plasma membrane fraction obtained from frog skeletal muscles was increased approximately two-fold by exposing muscles to insulin, whereas the addition of insulin to a membrane preparation suspension has no effect on Na,K-ATPase activity. The effect of insulin on Na,K-ATPase activity of whole muscles was specific to insulin and insulin derivatives that had the ability of receptor-binding and was not inhibited by actinomycin D. Insulin also induced a development of Na,K-ATPase activity in muscles whose Na,K-ATPase activity had been blocked by ouabain-pretreating. Such a insulin action was inhibited by monensin. These observations suggest that insulin stimulates the monensin-sensitive intracellular transport of membrane proteins which should be responsible for the increase in Na/K pumping activity.
D000818:Animals; D002451:Cell Compartmentation / Q000187:drug effects; D003609:Dactinomycin / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology*; D007329:Insulin Antagonists; D008861:Microsomes / Q000201:enzymology; D008928:Mitochondria / Q000201:enzymology; D008985:Monensin / Q000494:pharmacology; D009132:Muscles / Q000201:enzymology*; D010042:Ouabain / Q000494:pharmacology; D011892:Rana catesbeiana; D012508:Sarcolemma / Q000201:enzymology*; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D013329:Structure-Activity Relationship
D007328:Insulin; D007329:Insulin Antagonists; D003609:Dactinomycin; D010042:Ouabain; D008985:Monensin; D000254:Sodium-Potassium-Exchanging ATPase