Demonstration of thiamine pyrophosphatase in human germ cells and Sertoli cells, a histochemical study.
The distribution of thiamine pyrophosphatase (TPPase) was studied in the human testicular biopsy tissue with the help of a modified histochemical gel method. The spermatogonia showed a large round strongly positive reaction zone in the supranuclear region. The pachytene spermatocytes exhibited a large semicircular TPPase reaction zone around the nucleus directing towards the lumen. The early round spermatids were characterized by a small, round, and weak supranuclear reaction zone. The elongated late spermatids were devoid of TPPase activity. The Sertoli cells possessed a "streamer" like formation of strong TPPase activity spreading from basal to apical portion.
D006651:Histocytochemistry; D006801:Humans; D008297:Male; D011755:Pyrophosphatases / Q000378:metabolism*; D012708:Sertoli Cells / Q000201:enzymology* / Q000648:ultrastructure; D013087:Spermatids / Q000201:enzymology; D013090:Spermatocytes / Q000201:enzymology; D013093:Spermatogonia / Q000201:enzymology; D013094:Spermatozoa / Q000201:enzymology* / Q000648:ultrastructure; D013737:Testis / Q000201:enzymology; D013834:Thiamine Pyrophosphatase / Q000378:metabolism*; D014018:Tissue Distribution
D011755:Pyrophosphatases; D013834:Thiamine Pyrophosphatase