Cell nuclei generate DNA-nicking superoxide radicals.
Rat liver nuclei generate superoxide radicals in the presence of NADPH. Active oxygen species induced nicks in nuclear DNA. This was prevented by superoxide dismutase and catalase as well as by anaerobiosis. EDTA-Fe3+ dramatically increased the active oxygen-dependent DNA nicking.
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism*; D004264:DNA Topoisomerases, Type I / Q000096:biosynthesis*; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D004492:Edetic Acid / Q000494:pharmacology; D004587:Electrophoresis, Agar Gel; D005609:Free Radicals; D006861:Hydrogen Peroxide / Q000378:metabolism; D007501:Iron / Q000494:pharmacology; D008099:Liver / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013481:Superoxides / Q000378:metabolism*
D005609:Free Radicals; D013481:Superoxides; D009249:NADP; D004492:Edetic Acid; D006861:Hydrogen Peroxide; D007501:Iron; D004259:DNA-Directed DNA Polymerase; D004264:DNA Topoisomerases, Type I; D010100:Oxygen