Adenosine cyclic 3',5'-monophosphate and steroid production by small ovarian follicles from Booroola ewes with and without a fecundity gene.
The tissue contents of adenosine cyclic 3',5'-monophosphate (cAMP) in freshly dissected follicles (0.13-1.00 mm diam.) were significantly higher in Booroola ewes containing a major fecundity gene (FF and F+ ewes) compared to those values in Booroolas with no copy of the gene (++ animals; P less than 0.025). After a 1 h incubation with LH + FSH, the respective proportions of follicles with a diameter of 0.13-0.52 mm (n = 288) and 0.53-1.00 mm (n = 271) that had synthesized greater than or equal to 0.6 pmol cAMP and greater than or equal to 1.0 pmol cAMP were significantly influenced by genotype (Booroola ewes homozygous for the F-gene, FF greater than heterozygous, F+ greater than ++; P less than 0.01 for both follicle size ranges). The contents of progesterone, androstenedione, testosterone and oestradiol-17 beta in minced ethanolic extracts of freshly dissected follicles (n = 188) were undetectable regardless of Booroola genotype. However, when follicles of 0.53-1.00 mm but not 0.13-0.52 mm diameter were cultured for 48 h with LH + FSH under 70 kPa of a 50% O2, 45% N2 and 5% CO2 gas mixture, the proportions that synthesized high levels of progesterone (greater than or equal to 4.0 ng), androstenedione (greater than or equal to 3 ng), and oestradiol (greater than or equal to 0.8 ng) were significantly influenced by genotype (FF greater than F+ greater than or equal to ++; P less than 0.05 for each steroid). No significant genotypic differences were noted for testosterone synthesis. Collectively, these results show that the Booroola F-gene has an influence on the maturation of ovarian follicles from an early stage of growth.
D000735:Androstenedione / Q000096:biosynthesis; D000818:Animals; D046508:Culture Techniques; D000242:Cyclic AMP / Q000096:biosynthesis*; D004958:Estradiol / Q000096:biosynthesis; D005260:Female; D005298:Fertility; D005796:Genes; D005838:Genotype; D012739:Gonadal Steroid Hormones / Q000096:biosynthesis*; D006080:Ovarian Follicle / Q000378:metabolism*; D011374:Progesterone / Q000096:biosynthesis; D012756:Sheep / Q000235:genetics* / Q000378:metabolism
D012739:Gonadal Steroid Hormones; D000735:Androstenedione; D011374:Progesterone; D004958:Estradiol; D000242:Cyclic AMP