A recombinant murine retrovirus for simian virus 40 large T cDNA transforms mouse fibroblasts to anchorage-independent growth.
A recombinant murine retrovirus containing the intact cDNA sequence for the simian virus 40 (SV40) large T antigen (T) was constructed by using the pZIPNeo SV(X)1 vector. Psi 2 packaging cells were then transfected, and G418-resistant clones were used to generate helper-free viral stocks. NIH 3T3 mouse fibroblasts infected by the recombinant T cDNA retrovirus were selected fro G418 resistance. Such cultures synthesized authentic SV40 T and were transformed to anchorage-independent growth at high efficiency. Therefore, this vector has allowed the study of the transformation properties of T under conditions of neutral drug selection and in the absence of SV40 small t antigen.
D000818:Animals; D000952:Antigens, Polyomavirus Transforming; D000957:Antigens, Viral, Tumor / Q000235:genetics / Q000502:physiology*; D002448:Cell Adhesion; D002460:Cell Line; D002472:Cell Transformation, Viral; D004247:DNA / Q000235:genetics; D004274:DNA, Recombinant; D004279:DNA, Viral / Q000235:genetics; D005347:Fibroblasts / Q000502:physiology; D005822:Genetic Vectors; D051379:Mice; D009856:Oncogene Proteins, Viral / Q000235:genetics / Q000502:physiology*; D013539:Simian virus 40 / Q000235:genetics* / Q000502:physiology
D000952:Antigens, Polyomavirus Transforming; D000957:Antigens, Viral, Tumor; D004274:DNA, Recombinant; D004279:DNA, Viral; D009856:Oncogene Proteins, Viral; D004247:DNA