Nonpermissive infection of lymphoblastoid cells by vesicular stomatitis virus. II. Effect on viral morphogenesis.
The human B-lymphoblastoid cell line Raji is nonpermissive for infection by vesicular stomatitis virus (VSV). The VSV particles released from Raji cells display a more heterogeneous distribution in equilibrium sucrose density gradients than particles released from BHK cells. The particles released from Raji cells contain approximately one-half to one-third as much viral matrix protein, relative to the nucleocapsid protein, as is normal. They also contain a higher proportion of the unglycosylated form of the G protein. The particles released from Raji cells are unstable and many disintegrate in the growth medium. Most of them deform when subjected to ultracentrifugation prior to fixation. The ratio of plaque-forming units to physical particles is much lower for the virions released from Raji cells.
D001402:B-Lymphocytes / Q000382:microbiology*; D002460:Cell Line; D004261:DNA Replication; D006801:Humans; D008562:Membrane Glycoproteins; D009024:Morphogenesis; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral; D014721:Vesicular stomatitis Indiana virus / Q000032:analysis / Q000254:growth & development* / Q000378:metabolism; D014759:Viral Envelope Proteins; D014763:Viral Matrix Proteins; D010948:Viral Plaque Assay; D014764:Viral Proteins / Q000032:analysis / Q000096:biosynthesis; D014771:Virion
C028391:G protein, vesicular stomatitis virus; D008562:Membrane Glycoproteins; D012367:RNA, Viral; D014759:Viral Envelope Proteins; D014763:Viral Matrix Proteins; D014764:Viral Proteins