In vitro metabolism of [3H]-peptide leukotrienes in human and ferret lung: a comparison with the guinea pig.
Fragmented lung tissue prepared from human, ferret and guinea pig converted [3H]-leukotriene C4 ([3H]-LTC4) to [3H]-LTD4 and [3H]-LTE4. [3H]-LTD4 was the major product recovered from incubations with human and guinea pig lung, whereas [3H]-LTE4 was the predominant metabolite in ferret. Kinetic analysis in human lung yielded a much faster rate for the conversion of [3H]-LTC4 to [3H]-LTD4+E4 than for the conversion of [3H]-LTD4 to [3H]-LTE4. In all three species serine-borate complex blocked the metabolism of [3H]-LTC4, whereas L-cysteine blocked the metabolism of [3H]-LTD4. These studies demonstrate that guinea-pig and human lungs have a similar metabolic pattern and capacity which is dissimilar to the ferret.
D000818:Animals; D001881:Borates / Q000494:pharmacology; D002335:Carnivora / Q000378:metabolism*; D003545:Cysteine / Q000494:pharmacology; D005289:Ferrets / Q000378:metabolism*; D006168:Guinea Pigs; D006801:Humans; D007700:Kinetics; D017999:Leukotriene E4; D008168:Lung / Q000187:drug effects / Q000378:metabolism*; D013189:SRS-A / Q000031:analogs & derivatives / Q000378:metabolism*; D012694:Serine / Q000494:pharmacology; D013045:Species Specificity
D001881:Borates; D013189:SRS-A; C040900:serine-borate complex; D012694:Serine; D017999:Leukotriene E4; D003545:Cysteine