Hydroxyl radicals are not involved in NADPH dependent microsomal lipid peroxidation.
NADPH dependent H2O2 formation in microsomes in the presence of chelated iron leads to formation of hydroxyl radicals. Enhancement of hydroxyl radical generation (via ferric-EDTA or sodium azide) did not result in a concomitant increase in lipid peroxidation; rather, a decrease was observed. Moreover, the hydroxyl radical scavenger DMSO did not inhibit lipid peroxidation. This comparison of hydroxyl radical formation with lipid peroxidation suggests that hydroxyl radicals do not play a part in NADPH-dependent lipid peroxidation.
D000818:Animals; D001386:Azides / Q000494:pharmacology; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D005290:Ferric Compounds / Q000494:pharmacology; D006878:Hydroxides / Q000378:metabolism*; D017665:Hydroxyl Radical; D008054:Lipid Peroxides / Q000378:metabolism*; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism*; D009249:NADP / Q000494:pharmacology*; D010084:Oxidation-Reduction; D051381:Rats; D011919:Rats, Inbred Strains; D019810:Sodium Azide
D001386:Azides; D005290:Ferric Compounds; D006878:Hydroxides; D008054:Lipid Peroxides; D017665:Hydroxyl Radical; D009249:NADP; D019810:Sodium Azide; D004492:Edetic Acid; C019179:Fe(III)-EDTA; D004121:Dimethyl Sulfoxide