Different complement and granulocyte activation in patients dialyzed with PMMA dialyzers.
Plasma C3a and C5a levels as well as plasma levels of granulocyte lactoferrin, granulocyte myeloperoxidase and granulocyte elastase in complex with alpha 1-proteinase inhibitor (E-alpha 1PI) were investigated in 10 patients (52.7 +/- 5.9 years) undergoing maintenance hemodialysis (39.4 +/- 12.4 months) with hollow fiber dialyzers made from polymethylmethacrylate. Plasma levels of lactoferrin increased from 166.5 +/- 28.5 to 712.5 +/- 165.9 ng/ml, myeloperoxidase from 59.0 +/- 15.3 to 210.5 +/- 33.9 ng/ml and E-alpha 1PI from 114.2 +/- 18.1 to 681.8 +/- 102.6 ng/ml during dialysis. In contrast, plasma C3a levels rose from 179.8 +/- 33.6 to maximal 276.2 +/- 45.4 ng/ml and C5a from 55.7 +/- 8.1 to maximal 101.1 +/- 14.8 ng/ml. Our data indicate that degranulation of granulocytes occurs during dialysis despite only little complement activation and mild initial granulocytopenia.
D001672:Biocompatible Materials; D001798:Blood Proteins; D003167:Complement Activation; D003176:Complement C3 / Q000032:analysis*; D015926:Complement C3a; D003182:Complement C5 / Q000032:analysis*; D015936:Complement C5a; D005260:Female; D006098:Granulocytes / Q000201:enzymology*; D006801:Humans; D007688:Kidneys, Artificial; D007781:Lactoferrin / Q000097:blood*; D007782:Lactoglobulins / Q000097:blood*; D008297:Male; D008567:Membranes, Artificial; D008768:Methylmethacrylates; D008875:Middle Aged; D010196:Pancreatic Elastase / Q000097:blood; D009195:Peroxidase / Q000097:blood*; D011480:Protease Inhibitors / Q000097:blood; D006435:Renal Dialysis; D014511:Uremia / Q000201:enzymology / Q000276:immunology / Q000628:therapy; D000515:alpha 1-Antitrypsin
D001672:Biocompatible Materials; D001798:Blood Proteins; D003176:Complement C3; D003182:Complement C5; D007782:Lactoglobulins; D008567:Membranes, Artificial; D008768:Methylmethacrylates; D011480:Protease Inhibitors; D000515:alpha 1-Antitrypsin; D015926:Complement C3a; D015936:Complement C5a; D009195:Peroxidase; D007781:Lactoferrin; D010196:Pancreatic Elastase