HLA-JY328: mapping studies and expression of a polymorphic HLA class I gene.
The JY328 clone was identified in a human genomic library using cDNA corresponding to mRNA for HLA-B7 as a probe. The L/328 cell line was established by cotransformation of mouse Ltk- cells with the herpes thymidine kinase gene and clone JY328. On Northern blots, RNA from L/328 strongly hybridized to an HLA class I probe, and an antigen was recognized by an anti-HLA class I framework antibody on the cell surface. A DNA probe corresponding to a segment of intron 7 was developed by comparing the nucleotide sequence of clone JY328 with that of other HLA class I-type genes. Using the radiolabeled probe to screen Southern blots of DNA from families with siblings exhibiting intra-HLA recombinations, a restriction fragment length polymorphism was revealed--a 1.4 kb BstE II band not present in all individuals. A corresponding fragment was apparent in the base sequence of clone JY328. The occurrence of this band on Southern blots established that JY328 maps distinct from and centromeric to the HLA-C locus and near to the HLA-B locus. Antibody absorption studies and cytotoxicity tests indicated that the JY328 gene product was not an HLA-B antigen but that it did specifically absorb CW7-specific antibody. In sum, these results suggest a novel, polymorphic HLA class I gene which expresses a product serologically similar to HLA-Cw7 but which does not map within the corresponding locus.
D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D001402:B-Lymphocytes / Q000032:analysis / Q000276:immunology; D004247:DNA / Q000032:analysis / Q000235:genetics; D004262:DNA Restriction Enzymes; D005819:Genetic Markers; D006680:HLA Antigens / Q000235:genetics*; D015796:HLA-B27 Antigen; D006801:Humans; D007739:L Cells / Q000032:analysis; D008285:Major Histocompatibility Complex; D051379:Mice; D011110:Polymorphism, Genetic; D012689:Sequence Homology, Nucleic Acid
D000954:Antigens, Surface; D005819:Genetic Markers; D006680:HLA Antigens; D015796:HLA-B27 Antigen; D004247:DNA; D004262:DNA Restriction Enzymes