Selection of transformed cells in serum-free media.
NIH3T3 cells grow in a serum-free basal nutrient medium supplemented with fibronectin, transferrin, insulin, epidermal growth factor (EGF) and high density lipoprotein (HDL). The individual omission from the serum-free medium of insulin, EGF, or HDL results in greatly reduced cell growth. These growth-restrictive conditions can be used to select for cells transformed with SV40, the polyomavirus middle T antigen gene, the activated human ras gene, and the mouse c-myc gene.
D000818:Animals; D000952:Antigens, Polyomavirus Transforming; D000957:Antigens, Viral, Tumor / Q000235:genetics; D001769:Blood; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002472:Cell Transformation, Viral; D003470:Culture Media; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005353:Fibronectins / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology; D008075:Lipoproteins, HDL / Q000494:pharmacology; D051379:Mice; D008809:Mice, Inbred C3H; D009856:Oncogene Proteins, Viral / Q000235:genetics; D009857:Oncogenes; D013539:Simian virus 40; D014168:Transferrin / Q000494:pharmacology
D000952:Antigens, Polyomavirus Transforming; D000957:Antigens, Viral, Tumor; D003470:Culture Media; D005353:Fibronectins; D007328:Insulin; D008075:Lipoproteins, HDL; D009856:Oncogene Proteins, Viral; D014168:Transferrin; D004815:Epidermal Growth Factor