Sliding-end-labelling. A method to avoid artifacts in nucleosome positioning.
A method, termed 'sliding-end-labelling', has been devised to avoid a frequent artifact in nucleosome positioning by indirect end labelling, namely the appearing of DNA fragments originated by two nuclease cuts, one of them lying within the region covered by the probe. The method is applied to the nucleosome positioning in the yeast SUC2 gene for invertase.
D004262:DNA Restriction Enzymes; D004271:DNA, Fungal / Q000032:analysis; D004587:Electrophoresis, Agar Gel; D008836:Micrococcal Nuclease; D009693:Nucleic Acid Hybridization; D009707:Nucleosomes / Q000032:analysis*; D012441:Saccharomyces cerevisiae / Q000235:genetics
D004271:DNA, Fungal; D009707:Nucleosomes; D004262:DNA Restriction Enzymes; D008836:Micrococcal Nuclease