title: 2.2-kilobase class of early transcripts encoded by cell-related sequences in human cytomegalovirus strain AD169.
abstract: In this report we describe the kinetics of appearance and fine mapping of a 2.2-kilobase (kb) class of transcripts arising from a region of the human cytomegalovirus genome which contains cell-related sequences. These transcripts are encoded by adjacent EcoRI fragments R and d (map units 0.682 to 0.713), located within the long unique segment of the genome. The 2.2-kb RNAs were first detected at 8h postinfection and appeared at comparable or slightly lower levels at 28 and 72 h postinfection. At late times (72 h) additional transcripts were detected with probes from this region. RNase, S1 nuclease, and exonuclease VII protection analyses of 8- and 28-h RNA indicated that the 2.2-kb RNAs had a complex spliced structure consisting of invariable 5' and internal exons and a heterogeneous 3' exon. The position of the 5' end of the RNA was determined with respect to the nucleotide sequence. Analysis of this sequence showed that the cell-related sequences were contained within a long open reading frame in the 5' exon.
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003587:Cytomegalovirus / Q000235:genetics*; D004720:Endonucleases; D005786:Gene Expression Regulation; D005814:Genes, Viral; D008970:Molecular Weight; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics*; D015719:Single-Strand Specific DNA and RNA Endonucleases; D013997:Time Factors; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins; D004720:Endonucleases; D015719:Single-Strand Specific DNA and RNA Endonucleases