Production of monoclonal antibodies against parainfluenza 3 virus and their use in diagnosis by immunofluorescence.
Monoclonal antibodies were produced against parainfluenza virus type 3 (PI-3) and used to identify PI-3 clinical isolates in cell culture and PI-3 antigen in cells obtained from nasopharyngeal (NP) washes of patients. Two (2E9 and 4G5) of the three monoclonal antibodies characterized reacted by immunoblotting with a 67,000-dalton PI-3 protein, and one antibody (4E5) reacted with two viral proteins in the range of 29,000 to 31,000 daltons. The three monoclonal antibodies did not cross-react by indirect immunofluorescence (IFA) with PI-1 or PI-2 and identified by IFA 18 isolates of PI-3 in cell culture. The 2E9 antibody reacted with PI-3 antigen in cells of 8 NP wash specimens that also yielded PI-3 in cell culture. Cells from 12 specimens reactive by IFA for respiratory syncytial virus, 1 specimen yielding adenovirus in cell culture, and 5 specimens yielding influenza virus were not reactive.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology; D000914:Antibodies, Viral / Q000096:biosynthesis / Q000276:immunology; D000956:Antigens, Viral / Q000032:analysis; D002460:Cell Line; D003429:Cross Reactions; D005455:Fluorescent Antibody Technique; D006801:Humans; D006825:Hybridomas; D051379:Mice; D008970:Molecular Weight; D009305:Nasopharynx / Q000276:immunology / Q000382:microbiology; D010224:Parainfluenza Virus 3, Human / Q000276:immunology* / Q000302:isolation & purification; D018184:Paramyxoviridae Infections / Q000175:diagnosis*; D018108:Respirovirus / Q000276:immunology*; D014764:Viral Proteins / Q000276:immunology
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D014764:Viral Proteins