Polyclonal anti-idiotypic opioid receptor antibodies generated by the monoclonal beta-endorphin antibody 3-E7.
Anti-idiotypic antibodies were raised in rabbits against the monoclonal beta-endorphin antibody 3-E7. These antibodies inhibit beta-endorphin binding to the 3-E7 antibody, binding of 3H-diprenorphine to solubilized opioid receptors and the binding of 125I-beta-endorphin to rat brain membranes. Exposure of NG-108CC15 hybrid cells to anti-idiotypic antibodies produces an opioid-like inhibition of PGE1-stimulated cAMP accumulation. These data suggest that the antibodies raised by the anti-idiotypic route both bind to and activate opioid receptors.
D000818:Animals; D000906:Antibodies / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001921:Brain / Q000378:metabolism; D004174:Diprenorphine / Q000378:metabolism; D004723:Endorphins / Q000276:immunology* / Q000378:metabolism; D006168:Guinea Pigs; D006822:Hybrid Cells / Q000378:metabolism; D007082:Ileum / Q000378:metabolism; D007130:Immunoglobulin Idiotypes / Q000276:immunology; D008297:Male; D008566:Membranes / Q000378:metabolism; D051379:Mice; D011863:Radioimmunoassay; D051381:Rats; D011957:Receptors, Opioid / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D014649:Vas Deferens / Q000378:metabolism; D001615:beta-Endorphin
D000906:Antibodies; D000911:Antibodies, Monoclonal; D004723:Endorphins; D007130:Immunoglobulin Idiotypes; D011957:Receptors, Opioid; D004174:Diprenorphine; D001615:beta-Endorphin