Recovery of viruses from water by a modified flocculation procedure for second-step concentration.
A reduction in virus recovery efficiencies stemming from a change in the commercial processing of powdered beef extract was reversed by the addition of Celite analytical filter aid. Supplementing beef extract with this silicate is recommended as a modification to the organic flocculation procedure for second-step concentration in monitoring for waterborne viruses. Considerable differences in virus recovery were found among lots of beef extract and Celite preparations; this indicates that the performance of each lot of these substances should be checked before use.
D000818:Animals; D017610:Calcium Compounds; D002460:Cell Line; D004770:Enterovirus / Q000302:isolation & purification*; D029822:Enterovirus B, Human / Q000302:isolation & purification*; D007616:Kaolin; D017633:Magnesium Silicates; D017955:Poliovirus / Q000302:isolation & purification*; D017640:Silicates; D012824:Silicic Acid; D012822:Silicon Dioxide; D014773:Virology / Q000379:methods; D014871:Water Microbiology
D017610:Calcium Compounds; D017633:Magnesium Silicates; D017640:Silicates; C005013:Florisil; D012824:Silicic Acid; D007616:Kaolin; D012822:Silicon Dioxide; C031293:calcium silicate