Genomic and antigenic comparison of an equine herpesvirus 1 (EHV 1) isolate from the 1983 Lippizan abortion storm with EHV 1 reference strains.
An EHV 1 isolate from the Lippizan Stud at Piber, which caused the abortion and paresis outbreak in 1983, was investigated using 3 known subtype 1 and 2 subtype 2 strains for comparison. Broad-scale restriction enzyme analysis as well as cross-neutralization with hyperimmune sera produced in rabbits were performed, and SDS-PAGE of infected cell proteins was conducted on a limited scale. The Piber isolate was clearly classified as a subtype 1 strain of EHV 1, and showed closest resemblance in its restriction patterns with a British EHV 1 strain, which originated from an outbreak with paretic symptoms. A second Piber isolate from the same outbreak examined to limited extent only was practically indistinguishable from the first one, as could have been expected. A thoroughly controlled systematic vaccination program with existing commercial vaccines against EHV 1 should protect the endangered Lippizan horses population against the abortigenic and less certainly against the paretic syndromes caused by this virus. According to data presented, a protection against respiratory disease is less probable.
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D002460:Cell Line; D002478:Cells, Cultured; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000235:genetics*; D005260:Female; D006564:Herpesviridae / Q000235:genetics*; D006566:Herpesviridae Infections / Q000662:veterinary; D004861:Herpesvirus 1, Equid / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D006734:Horse Diseases / Q000382:microbiology; D006736:Horses; D007668:Kidney; D008970:Molecular Weight; D011247:Pregnancy; D012867:Skin; D014764:Viral Proteins / Q000302:isolation & purification
D000956:Antigens, Viral; D004279:DNA, Viral; D014764:Viral Proteins; D004262:DNA Restriction Enzymes