Spectrin does not redistribute with actin during dBcAMP-induced changes in astrocytes in vitro.
Cells of the astrocyte lineage obtained from mouse neopallium and grown in colony culture have been investigated for a correlation between distributions of F-actin and the common subunit of an erythrocyte actin binding protein, alpha-spectrin (brain fodrin). The cells of the astrocyte lineage at the astroblast stage have F-actin organized in the form of prominent, linearly arranged microfilament bundles. We have demonstrated that spectrin in these cells forms a fine reticulum lining the cell cortex. During the dibutyryl cyclic (dBcAMP)-induced transition from astroblasts to reactive astrocytes, actin-containing microfilaments undergo the dramatic rearrangement from a predominantly linear to a predominantly circumferential spatial organization. remains in the form of a fine reticulum lining the cellular cortex. These remains in the form of a fine reticulum lining the cellular cortex. These findings support the recent notion that spectrin in non-erythroid cells is not essential for maintaining the organization and plasma membrane membrane anchorage of the prominent microfilament bundles.
D000199:Actins / Q000378:metabolism*; D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D003994:Bucladesine / Q000494:pharmacology*; D002454:Cell Differentiation; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000187:drug effects; D005455:Fluorescent Antibody Technique; D051379:Mice; D008811:Mice, Inbred DBA; D013049:Spectrin / Q000378:metabolism*; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism
D000199:Actins; D013049:Spectrin; D003994:Bucladesine