Isolation of a lentivirus from a macaque with lymphoma: comparison with HTLV-III/LAV and other lentiviruses.
A retrovirus has been isolated on the human T-cell line HuT 78 after cocultivation of a lymph node from a pig-tailed macaque (Macaca nemestrina) that had died with malignant lymphoma in 1982 at the University of Washington primate center. This isolate, designated MnIV (WPRC-1) (M. nemestrina immunodeficiency virus, Washington Primate Research Center) shows the characteristic morphology of a lentivirus and replicates to high titers in various lymphocyte lines of human and primate origin. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of purified MnIV revealed multiple bands of structural proteins, including a major viral gag protein of 28 kilodaltons, that did not comigrate with the viral proteins of a human immunodeficiency virus (HIV [FRE-1]) that was also isolated on HuT 78 cells. The relatedness of MnIV to other lentiviruses (HTLV-III/LAV, EIAV, and visna) was examined in radioimmunoassays, by immunoblot techniques, and by N-terminal amino acid sequence analysis of the viral p28 gag protein. The immunoassays revealed cross-reactivity only between MnIV p28 and HTLV-III/LAV p24, and sequence analysis showed that 14 of the 24 N-terminal residues of MnIV p28 and HTLV-III/LAV p24 are identical. These results indicate that MnIV belongs to the same lentivirus family as HTLV-III/LAV but is only partially related to these human acquired immune deficiency syndrome retroviruses.
D000595:Amino Acid Sequence; D000818:Animals; D000956:Antigens, Viral / Q000276:immunology; D003429:Cross Reactions; D017977:Deltaretrovirus / Q000032:analysis / Q000276:immunology; D015683:Gene Products, gag; D006678:HIV / Q000032:analysis / Q000276:immunology; D008223:Lymphoma / Q000382:microbiology / Q000662:veterinary*; D008254:Macaca nemestrina; D008970:Molecular Weight; D008992:Monkey Diseases / Q000382:microbiology*; D012190:Retroviridae / Q000032:analysis / Q000145:classification / Q000276:immunology / Q000302:isolation & purification*; D012191:Retroviridae Proteins / Q000032:analysis / Q000276:immunology
D000956:Antigens, Viral; D015683:Gene Products, gag; D012191:Retroviridae Proteins