[Characteristics of the interaction of restriction endonuclease BamHI with oligodeoxynucleotides].
Interaction of the restriction endonuclease BamHI with a series of synthetic oligodeoxynucleotides containing the restriction site has been studied. The enzyme is shown to specifically cut the BamHI site in hexanucleotide (I) and in non-selfcomplementary deca- and octanucleotides (II)-(IV). The data obtained led to the conclusion that BamHI reacts with duplex structures, while playing an important role in their stabilization. In 14-mer (V) BamHI cuts a non-standard half-site GAA to yield the 5'-terminal tri- (rather than hepta-) nucleotide. Hypothetical mechanisms of the process are discussed basing on conception of the role of higher DNA structures in the interaction with restriction endonucleases.
D001483:Base Sequence; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D015245:Deoxyribonuclease BamHI; D004591:Electrophoresis, Polyacrylamide Gel; D066298:In Vitro Techniques; D046911:Macromolecular Substances; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000378:metabolism*
D046911:Macromolecular Substances; D009838:Oligodeoxyribonucleotides; D004262:DNA Restriction Enzymes; D015245:Deoxyribonuclease BamHI
Osobennosti vzaimodeĭstviia éndonukleazy restriktsii BamHI s oligodezoksinukleotidami.