D1 protein of Drosophila melanogaster. Purification and AT-DNA binding properties.
D1 protein of Drosophila melanogaster is a sequence-specific DNA-binding protein which recognizes AT-rich DNA sequences. AT-rich DNA sequences in eukaryotic organisms are distributed in two characteristic ways: flanking transcriptional units and in constitutive heterochromatin. D1 could play a role in regulation of gene expression and in geographical localization of DNA sequences within the nucleus. D1 has been partially purified by ion exchange chromatography. DNA-binding activity was investigated by nucleoprotein gel electrophoresis, using end-labeled restriction fragments varying in AT sequence content. D1 binds most tightly to the satellite sequence -AATAT-, with intermediate strength to the complex satellite (359-base pair repeat) and another AT-rich (68% AT) mixed sequence DNA, and least to the simple satellite sequence -AAGAG-.
D000225:Adenine; D000818:Animals; D001483:Base Sequence; D002467:Cell Nucleus / Q000032:analysis; D002478:Cells, Cultured; D002852:Chromatography, Ion Exchange; D004247:DNA / Q000378:metabolism*; D004262:DNA Restriction Enzymes; D004268:DNA-Binding Proteins / Q000302:isolation & purification / Q000378:metabolism*; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D007457:Iodine Radioisotopes; D046911:Macromolecular Substances; D009698:Nucleoproteins / Q000378:metabolism; D009994:Osmolar Concentration; D010472:Perchlorates; D012995:Solubility; D013941:Thymine
C046995:D1 protein, Drosophila; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D007457:Iodine Radioisotopes; D046911:Macromolecular Substances; D009698:Nucleoproteins; D010472:Perchlorates; D004247:DNA; D004262:DNA Restriction Enzymes; D000225:Adenine; D013941:Thymine