Endonucleolytic activity that cleaves immunoglobulin recombination sequences.
An endonucleolytic activity has been identified in nuclear extracts of chick embryo bursa and mouse fetal liver cells. The activity introduces a double-strand cut in the vicinity of the recombination site of immunoglobulin joining gene segments. The cleavage occurs at the dinucleotide pair TG-AC. This activity is a good candidate for the putative endonuclease involved in recombination of the immunoglobulin variable, diversity, and joining regions. It is distinct from the endonuclease activities previously reported by others.
D000818:Animals; D001483:Base Sequence; D002060:Bursa of Fabricius / Q000201:enzymology; D002642:Chick Embryo; D004720:Endonucleases / Q000378:metabolism*; D007144:Immunoglobulin J-Chains / Q000235:genetics; D007135:Immunoglobulin Variable Region / Q000235:genetics; D007136:Immunoglobulins / Q000235:genetics*; D008099:Liver / Q000201:enzymology; D051379:Mice; D011995:Recombination, Genetic
D007144:Immunoglobulin J-Chains; D007135:Immunoglobulin Variable Region; D007136:Immunoglobulins; D004720:Endonucleases