Insertion of several different DNAs in reticuloendotheliosis virus strain T suppresses transformation by reducing the amount of subgenomic mRNA.
The highly oncogenic retrovirus reticuloendotheliosis virus (Rev) strain T (Rev-T) has, relative to its helper virus Rev strain A, a substitution of the oncogene v-rel for most of the env gene and a large deletion of gag and pol sequences. When the helper virus sequences that are deleted in Rev-T are replaced, the recombinant virus is nontransforming (I. S. Y. Chen and H. M. Temin, Cell 31: 111-120, 1982). We show that suppression of transformation occurs when several different DNA sequences are inserted in Rev-T and that suppression is correlated with a reduction in the amount of v-rel mRNA and v-rel protein in infected cells. The reduced amount of v-rel protein is insufficient for transformation.
D000818:Animals; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D002642:Chick Embryo; D004279:DNA, Viral / Q000235:genetics*; D003673:Defective Viruses / Q000235:genetics; D005786:Gene Expression Regulation; D005814:Genes, Viral; D009856:Oncogene Proteins, Viral / Q000235:genetics*; D009857:Oncogenes; D012326:RNA Splicing; D001356:Reticuloendotheliosis virus / Q000235:genetics*; D012190:Retroviridae / Q000235:genetics*; D014779:Virus Replication
D004279:DNA, Viral; D009856:Oncogene Proteins, Viral