Norepinephrine decreases EGF binding in primary rat hepatocyte cultures.
Norepinephrine (NE) produced a dose-dependent inhibition of 125I-epidermal growth factor (EGF) binding to adult rat hepatocytes in primary culture. This effect was maximal after 1 hr of incubation with NE and could be blocked by the presence of an alpha 1-specific adrenergic receptor antagonist. The inhibition of binding correlates with the ability of NE to enhance hepatocyte DNA synthesis in the presence of EGF and appears to be mediated by a reduction in EGF receptor number, without a significant change in receptor affinity.
D000818:Animals; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D004815:Epidermal Growth Factor / Q000378:metabolism*; D066246:ErbB Receptors; D005260:Female; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D009638:Norepinephrine / Q000494:pharmacology*; D010869:Pindolol / Q000494:pharmacology; D011224:Prazosin / Q000494:pharmacology; D051381:Rats; D011916:Rats, Inbred F344; D011956:Receptors, Cell Surface / Q000378:metabolism*; D015016:Yohimbine / Q000494:pharmacology
D011956:Receptors, Cell Surface; D015016:Yohimbine; D004815:Epidermal Growth Factor; D004247:DNA; D010869:Pindolol; D066246:ErbB Receptors; D009638:Norepinephrine; D011224:Prazosin