The effect of zinc in vivo and in vitro on the activities of angiotensin converting enzyme and kininase-I in the plasma of rats.
Two groups of rats were pair fed, for 18 days, diets containing either 2.6 (Zn deficient) or 100 mg Zn/kg (control diet). Plasma was assayed spectrophotometrically for the activity of kininase-I and angiotensin converting enzyme (ACE) in the presence of varying concentrations of added Zn2+. Zinc deficient rats had only 76% of the activity of both kininase-I and ACE compared with zinc supplemented control rats. There was a significant linear relationship between enzyme activity and concentration of zinc in plasma for both enzymes. When zinc was added to the enzyme incubation mixture for zinc deficient rats, the activity of ACE increased by 73% and that of kininase-I by 33%. This Zn2+-stimulated increase in enzyme activity was negatively correlated with the in vivo concentration of zinc in plasma, and a plateau in enzyme activity was seen at concentrations of plasma zinc that were commensurate with normal zinc status (over 14 mumol/1). The results demonstrate that the activities of both kininase-I and ACE are dependent on the concentration of zinc in vivo and in vitro, and suggest that information concerning the concentration of zinc in plasma and assay solutions be a prerequisite solutions be a prerequisite for the use of these enzymes in the clinical diagnosis of disease states. The results also showed that the activity of ACE and kininase-I in plasma could be used for the biochemical diagnosis of a suboptimal zinc status.
D000806:Angiotensin-Converting Enzyme Inhibitors; D000818:Animals; D002268:Carboxypeptidases / Q000097:blood*; D004656:Enalapril / Q000031:analogs & derivatives / Q000494:pharmacology; D015773:Enalaprilat; D005260:Female; D001121:Lysine Carboxypeptidase / Q000037:antagonists & inhibitors / Q000097:blood*; D008297:Male; D007703:Peptidyl-Dipeptidase A / Q000097:blood*; D010618:Phenanthrolines / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D015032:Zinc / Q000097:blood / Q000494:pharmacology*
D000806:Angiotensin-Converting Enzyme Inhibitors; D010618:Phenanthrolines; D004656:Enalapril; D002268:Carboxypeptidases; D007703:Peptidyl-Dipeptidase A; D001121:Lysine Carboxypeptidase; D015773:Enalaprilat; D015032:Zinc; C025205:1,10-phenanthroline