Hirschsprung's disease: catecholamine content, alpha-adrenoceptors, and the effect of electrical stimulation in aganglionic colon.
In order to assess abnormalities in the adrenergic mechanism in the intestine of Hirschsprung's disease, catecholamine concentrations, alpha-adrenoceptors, and the effect of electrical field stimulation were examined in aganglionic segments of colon or rectum. The aganglionic segment had a higher concentration of norepinephrine, assayed with high performance liquid chromatography with an electrochemical detector, whereas concentrations of epinephrine or dopamine were similar in normal and pathological segments. In four patients with extensive aganglionosis, the norepinephrine concentration in aganglionic colon segments decreased progressively in descending, transverse, and ascending colon. The tissue content of alpha-adrenoceptors and their affinity assayed from the specific binding of [3H]dihydro-alpha-ergocryptine appeared similar in normal and aganglionic segments of the rectosigmoidal colon. Electrical field stimulation of normal rectosigmoidal colon segments caused relaxation at low frequencies and contraction at a very high frequency. Relaxation was not abolished by blocking concentrations of propranolol or phentolamine. In aganglionic segments, the predominant response to electrical field stimulation was contraction, which was inhibited by either atropine or tetrodotoxin. These results indicate that an alpha-adrenergic system and cholinergic innervation apparently exist in aganglionic colon segments and that dysfunction of the colon appears to result from lack of a nonadrenergic inhibitory system.
D001285:Atropine / Q000494:pharmacology; D002395:Catecholamines / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D003106:Colon / Q000187:drug effects / Q000294:innervation / Q000503:physiopathology*; D004298:Dopamine / Q000378:metabolism; D004558:Electric Stimulation; D004837:Epinephrine / Q000378:metabolism; D005726:Ganglia, Parasympathetic / Q000002:abnormalities*; D006627:Hirschsprung Disease / Q000378:metabolism* / Q000503:physiopathology; D006801:Humans; D009638:Norepinephrine / Q000378:metabolism; D010646:Phentolamine / Q000494:pharmacology; D011433:Propranolol / Q000494:pharmacology; D011942:Receptors, Adrenergic, alpha / Q000378:metabolism*; D013779:Tetrodotoxin / Q000494:pharmacology
D002395:Catecholamines; D011942:Receptors, Adrenergic, alpha; D013779:Tetrodotoxin; D001285:Atropine; D011433:Propranolol; D004298:Dopamine; D009638:Norepinephrine; D004837:Epinephrine; D010646:Phentolamine