Transformation of Streptomyces granaticolor with natural and recombinant plasmid vectors.
Protoplasts of Streptomyces granaticolor were found to be transformable by the broad-host-range plasmid pIJ350 but no transformants were detected when the narrow-host-range plasmid pIJ2 or the shuttle vector pPM66 (pIJ350--pBR322) isolated from E. coli cells were used. The onset of blue colour granaticin production by S. granaticolor cells was used as a marker to prepare protoplasts with a high transformation capacity. The presence of a restriction system is discussed.
D004262:DNA Restriction Enzymes; D010957:Plasmids; D011995:Recombination, Genetic; D013171:Spores, Bacterial; D013302:Streptomyces / Q000235:genetics* / Q000254:growth & development / Q000502:physiology; D014169:Transformation, Bacterial
D004262:DNA Restriction Enzymes