A peculiar repetitive sequence in the rat genome.
We report here a new type of peculiar repetitive sequence, A15T(TC)9T12, which was detected at 750 base pairs (bp) upstream of a rat calmodulin processed pseudogene by DNA sequencing of cloned DNA fragments. This sequence element could possibly form a cruciform structure with a 12-AT-pair stem, exposing (CT)9 sequences as a loop. S1 nuclease protection experiments failed to identify this element as a cruciform structure but instead detected an alternating purine pyrimidine tract at 50 bp downstream of this element. Total genomic Southern blotting showed that the rat genome contains only a few of these elements.
D000818:Animals; D001483:Base Sequence; D002147:Calmodulin / Q000235:genetics; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant; D004720:Endonucleases; D005796:Genes; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D051381:Rats; D012091:Repetitive Sequences, Nucleic Acid; D015719:Single-Strand Specific DNA and RNA Endonucleases
D002147:Calmodulin; D004274:DNA, Recombinant; D004720:Endonucleases; D004262:DNA Restriction Enzymes; D015719:Single-Strand Specific DNA and RNA Endonucleases