Hemagglutination with ovine rotavirus. Brief report.
The virus was grown in MA 104 cells, a stable cell line derived from embryonic rhesus monkey kidney, and tested for hemagglutination (HA) with erythrocytes of a variety of species at 4 degrees C, room temperature and 37 degrees C. HA was observed at all temperatures with chicken, sheep, rabbit, guinea pig and human erythrocytes but not with horse, cattle, goat, swine and goose erythrocytes. HA reaction was inhibited by specific antiserum. Some factors involved in the HA and HA-inhibition (HI) were investigated and standard HA and HI tests were worked out. The HI test as well as neutralization test clearly distinguished ovine rotavirus from bovine, human, simian, equine, porcine and lapine rotaviruses.
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D002460:Cell Line; D002645:Chickens; D004912:Erythrocytes; D005777:Geese; D006041:Goats; D006168:Guinea Pigs; D006385:Hemagglutination Inhibition Tests; D006386:Hemagglutination Tests; D006389:Hemagglutinins, Viral / Q000502:physiology; D006736:Horses; D006801:Humans; D007668:Kidney; D008253:Macaca mulatta; D011817:Rabbits; D012401:Rotavirus / Q000276:immunology / Q000302:isolation & purification / Q000502:physiology*; D012756:Sheep / Q000382:microbiology*; D013045:Species Specificity
D000914:Antibodies, Viral; D006389:Hemagglutinins, Viral