An ESR study of the effect of an electrostatic field on binding of divalent cations to the surface of serum low-density lipoproteins.
The ESR technique has been used to study binding of Mn(II) ions to low-density lipoprotein (LDL) in solutions of various electrolyte ionic strengths. A model of the binding has been proposed which describes all the observations in electrolytes of ten different concentrations in terms of two types of binding sites and two corresponding sets of intrinsic binding parameters (n1 = 8, Kd1 = 1.31 X 10(-3) mol X l-1 and n2 = 170, Kd2 = 5.71 X 10(-2) mol X l-1). These parameters, together with the values of the potential (phi 0) responsible for binding of the ions to specific charged sites on the surface, reproduce the observed binding curves well in all the systems studied. The phi 0 values are obtained as an appropriate solution of the Poisson-Boltzmann equation.
D001665:Binding Sites; D002413:Cations, Divalent; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods; D006801:Humans; D007700:Kinetics; D008077:Lipoproteins, LDL / Q000097:blood*; D008345:Manganese / Q000097:blood*; D011485:Protein Binding
D002413:Cations, Divalent; D008077:Lipoproteins, LDL; D008345:Manganese