Purification of a factor specific for the upstream element of the adenovirus-2 major late promoter.
Stimulation of in vitro transcription by the upstream element (UE) of the adenovirus-2 major late promoter (Ad2MLP) involves a specific trans-acting factor present in a HeLa whole-cell extract. By following its transcriptional stimulatory activity and its DNase I footprint on the Ad2MLP-UE, we have purified this factor to greater than 10% purity and separated it from RNA polymerase B and the general transcription factors required for transcription from the Ad2MLP.
D000260:Adenoviruses, Human / Q000235:genetics*; D002845:Chromatography / Q000379:methods; D002850:Chromatography, Gel / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D017886:Durapatite; D005796:Genes; D005814:Genes, Viral; D006367:HeLa Cells / Q000378:metabolism; D006801:Humans; D006882:Hydroxyapatites; D011401:Promoter Regions, Genetic; D014157:Transcription Factors / Q000302:isolation & purification*; D014158:Transcription, Genetic
D006882:Hydroxyapatites; D014157:Transcription Factors; D017886:Durapatite