Iron respiration-driven proton translocation in aerobic bacteria.
Washed cell suspensions of Aquaspirillum magnetotacticum MS-1, A. itersonii E12639, Bacillus subtilis 6633, and Escherichia coli CSH27 translocated protons in response to the added oxidant O2 or NO3-, with triphenylmethylphosphonium bromide as the permeant ion. Iron respiration-driven proton translocation was observed in A. magnetotacticum MS-1, B. subtilis, and E. coli but not in a nonmagnetic strain of A. magnetotacticum (strain NM-1A) or with A. itersonii. Proton translocation to Fe3+ was totally inhibited by 500 microM NaN3 or 0.5 microM carbonyl cyanide m-chlorophenylhydrazone.
D000332:Aerobiosis; D001412:Bacillus subtilis / Q000502:physiology*; D018407:Bacterial Physiological Phenomena; D001692:Biological Transport; D004926:Escherichia coli / Q000502:physiology*; D007501:Iron / Q000502:physiology*; D050901:Nitrate Reductase; D009565:Nitrate Reductases / Q000378:metabolism; D010084:Oxidation-Reduction; D011522:Protons
D011522:Protons; D007501:Iron; D009565:Nitrate Reductases; D050901:Nitrate Reductase