DNATYPE--a personal computer program for HLA-DR typing based upon restriction fragment length polymorphisms.
The use of specific cDNA probes and selected combinations of restriction endonucleases makes it possible to characterize specific antigens by the molecular weights of the hybridized restriction enzyme digests of DNA. We have developed a simple program suitable for personal computers which utilizes such results to deduce HLA-DR phenotypes of any nucleated cell from which DNA may be extracted.
D003201:Computers; D004262:DNA Restriction Enzymes; D005838:Genotype; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II / Q000235:genetics*; D006650:Histocompatibility Testing / Q000379:methods; D006801:Humans; D008214:Lymphocytes; D011110:Polymorphism, Genetic; D012984:Software
D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II; D004262:DNA Restriction Enzymes