Transcriptional regulation of the transferrin receptor in differentiating HL-60 leukemic cells.
HL-60 promyelocytic leukemia cells were induced to differentiate along the granulocytic pathway by treatment with dimethylsulfoxide. A significant decrease in transferrin receptor specific mRNA was seen as early as 4 hr after exposure to inducer. Relative to untreated controls, transcript levels decreased 5-fold within 24 hr of exposure to dimethylsulfoxide and remained depressed throughout a 6-day treatment period. Reductions in receptor transcripts preceded the loss of surface receptor which in turn preceded the expression of the differentiated phenotype. The findings demonstrate that differentiation-associated regulation of the transferrin receptor can occur at the transcriptional level.
D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D005455:Fluorescent Antibody Technique; D006801:Humans; D007700:Kinetics; D015470:Leukemia, Myeloid, Acute; D012333:RNA, Messenger / Q000235:genetics; D011956:Receptors, Cell Surface / Q000235:genetics* / Q000378:metabolism; D011990:Receptors, Transferrin; D014158:Transcription, Genetic; D014168:Transferrin / Q000378:metabolism
D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D011990:Receptors, Transferrin; D014168:Transferrin; D004121:Dimethyl Sulfoxide