title: Phosphorylated threonine as the covalent intermediate in snake venom phosphodiesterase.
abstract: The covalent intermediate of snake venom phosphodiesterase has been isolated using thymidine 5'-[alpha-32P]triphosphate as substrate. Phosphoamino acid analysis of the labeled enzyme demonstrates that threonine is the active site residue forming the covalent intermediate. 5'-Nucleotide phosphodiesterase is the first enzyme reported to have an active site threonine forming a covalent intermediate.
mesh_terms: D001345:Autoradiography; D001665:Binding Sites; D003435:Crotalid Venoms / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D006868:Hydrolysis; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism*; D010766:Phosphorylation; D013912:Threonine / Q000378:metabolism*
chemical_list: D003435:Crotalid Venoms; D013912:Threonine; D010727:Phosphoric Diester Hydrolases