Regulation of canalicular bile formation by alpha-adrenergic action and by external ATP in the isolated perfused rat liver.
In isolated perfused rat liver, addition of adrenaline induced a complex response of bile flow including rapid, reversible stimulation (1/2-2 min), reversible inhibition (2-10 min), and prolonged stimulation. Both the reversible stimulation and the inhibition were mimicked by the alpha-sympathomimetic agonist phenylephrine but not by the beta-agonist isoproterenol. The reversible stimulation was a very early effect being terminated prior to all other alpha-adrenergic responses of liver. External ATP considerably lowered bile flow while inducing release of glucose and lactate, inhibition of respiration, and a reversible efflux of Ca2+. Variations of mannitol clearance parallel to those of bile flow indicate a canalicular origin of all changes.
D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000818:Animals; D001646:Bile / Q000378:metabolism*; D001648:Bile Canaliculi / Q000187:drug effects / Q000378:metabolism*; D001653:Bile Ducts, Intrahepatic / Q000378:metabolism*; D001663:Bilirubin / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D004837:Epinephrine / Q000494:pharmacology; D007545:Isoproterenol / Q000494:pharmacology; D007700:Kinetics; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D010656:Phenylephrine / Q000494:pharmacology; D011188:Potassium / Q000378:metabolism; D051381:Rats; D011942:Receptors, Adrenergic, alpha / Q000502:physiology*
D011942:Receptors, Adrenergic, alpha; D010656:Phenylephrine; D000255:Adenosine Triphosphate; D007545:Isoproterenol; D001663:Bilirubin; D011188:Potassium; D002118:Calcium; D004837:Epinephrine