title: Purification of the human thyroid peroxidase and its identification as the microsomal antigen involved in autoimmune thyroid diseases.
abstract: Human thyroid peroxidase (TPO) has been purified from thyroid microsomes by immunoaffinity chromatography using a monoclonal antibody (mAb) to TPO. The eluted material had a specific activity of 381 U/mg and exhibited a peak in the Soret region. The ratio of A411 to A280 ranged from 0.20 to 0.25. Upon SDS-polyacrylamide gel electrophoresis, the purified enzyme gave two contiguous bands in the 100 kDa region. Further, it has been demonstrated that sera with anti-microsomal autoantibodies from patients presenting Graves' or Hashimoto's thyroiditis diseases were able to bind to purified TPO and to inhibit in a dose-dependent manner the mAb binding to purified TPO. This suggests that TPO is the thyroid antigen termed to date the microsomal antigen.
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D000941:Antigens / Q000302:isolation & purification*; D001324:Autoantigens / Q000302:isolation & purification*; D001327:Autoimmune Diseases / Q000276:immunology*; D055598:Chemical Phenomena; D011232:Chemical Precipitation; D002621:Chemistry; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007120:Immunochemistry; D007527:Isoenzymes / Q000276:immunology / Q000302:isolation & purification*; D008861:Microsomes / Q000201:enzymology / Q000276:immunology; D009195:Peroxidase; D010544:Peroxidases / Q000276:immunology / Q000302:isolation & purification*; D012995:Solubility; D013959:Thyroid Diseases / Q000276:immunology*; D013961:Thyroid Gland / Q000201:enzymology* / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D000941:Antigens; D001324:Autoantigens; D007527:Isoenzymes; D010544:Peroxidases; D009195:Peroxidase