Polypeptides coded for by the region pre-S and gene S of hepatitis B virus DNA with the receptor for polymerized human serum albumin: expression on hepatitis B particles produced in the HBeAg or anti-HBe phase of hepatitis B virus infection.
There are four polypeptides coded for by the region Pre-S and gene S on DNA of hepatitis B virus that carry the receptor for polymerized human serum albumin (poly-HSA), i.e., P31 and P39, as well as their glycosylated counterparts P35 and P43. With the use of monoclonal antibodies directed to Pre-S(1) sequence and Pre-S(2) sequence (bearing the receptor for poly-HSA), the content of these polypeptides, as well as their expression on the surface, was determined for hepatitis B particles of various categories. P39 and P43, carrying both Pre-S(1) and Pre-S(2) sequences, were contained abundantly in Dane and tubular particles, and to a much lesser extent in small spherical particles, all of which were purified from plasma containing hepatitis B e antigen (HBeAg). P31 and P35, carrying Pre-S(2) but not Pre-S(1) sequence, were contained comparably in these three categories of hepatitis B particles. In remarkable contrast, small spherical particles derived from plasma containing antibody to HBeAg were very low in the content of any Pre-S polypeptides. P31 and P39 showed higher activities for poly-HSA receptor than their glycosylated versions. When Dane particles were digested with trypsin, the poly-HSA receptor was deprived in parallel with the loss of antigenicity for Pre-S(2) sequence. The antigenicity for Pre-S(1) sequence was much less affected, and that for the product of gene S was virtually unchanged by the digestion.
D000595:Amino Acid Sequence; D000937:Antigen-Antibody Reactions; D004279:DNA, Viral / Q000032:analysis; D005814:Genes, Viral; D006509:Hepatitis B / Q000276:immunology / Q000382:microbiology*; D006510:Hepatitis B Antibodies / Q000096:biosynthesis*; D006511:Hepatitis B Antigens / Q000235:genetics*; D006513:Hepatitis B e Antigens / Q000235:genetics* / Q000276:immunology; D006801:Humans; D011498:Protein Precursors / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D017457:Receptors, Albumin; D011956:Receptors, Cell Surface / Q000032:analysis*; D012709:Serum Albumin / Q000378:metabolism*; D014764:Viral Proteins / Q000032:analysis* / Q000235:genetics / Q000276:immunology
D004279:DNA, Viral; D006510:Hepatitis B Antibodies; D006511:Hepatitis B Antigens; D006513:Hepatitis B e Antigens; D011498:Protein Precursors; D017457:Receptors, Albumin; D011956:Receptors, Cell Surface; D012709:Serum Albumin; D014764:Viral Proteins