Effect of high crude protein on pituitary and ovarian function in Holstein cows.
Intact and ovariectomized cows were used to determine the effect of high amounts of dietary crude protein on pituitary regulation of luteinizing hormone. Estrus was synchronized with prostaglandin F2 alpha in 10 intact dry cows fed 15 or 25% crude protein, and serum luteinizing hormone profiles were evaluated during both follicular and luteal phases of the estrous cycle. Serum progesterone, pituitary luteinizing hormone content, and luteinizing hormone-releasing hormone receptors were also measured. Basal concentrations of serum luteinizing hormone tended to be lower during the follicular phase and were significantly higher during the luteal phase in cows fed 25% crude protein. Serum progesterone was not affected by dietary treatment. In 10 ovariectomized cows fed 24% crude protein, amplitude of luteinizing hormone response to luteinizing hormone-releasing hormone challenge tended to be smaller than that of 8 cows fed 16% crude protein, although total luteinizing hormone released in response to luteinizing hormone releasing hormone challenge was not different between dietary treatments. In both intact and ovariectomized cows, pulsatile luteinizing hormone patterns, pituitary luteinizing hormone content, and luteinizing hormone-releasing hormone receptors were not influenced by treatment. High dietary crude protein did not have a primary effect on luteinizing hormone or progesterone in nonlactating cows.
D000641:Ammonia / Q000032:analysis; D000821:Animal Feed; D000818:Animals; D002417:Cattle / Q000502:physiology*; D004044:Dietary Proteins / Q000494:pharmacology*; D005260:Female; D005640:Follicle Stimulating Hormone / Q000097:blood*; D007987:Gonadotropin-Releasing Hormone / Q000494:pharmacology; D007986:Luteinizing Hormone / Q000097:blood*; D010052:Ovariectomy; D010053:Ovary / Q000187:drug effects*; D010903:Pituitary Gland, Anterior / Q000187:drug effects*; D011374:Progesterone / Q000097:blood; D011956:Receptors, Cell Surface / Q000032:analysis; D011966:Receptors, LHRH; D012417:Rumen / Q000032:analysis
D004044:Dietary Proteins; D011956:Receptors, Cell Surface; D011966:Receptors, LHRH; D007987:Gonadotropin-Releasing Hormone; D011374:Progesterone; D000641:Ammonia; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone