title: Characterization of rabbit genes for synovial cell collagenase.
abstract: To provide tools for understanding collagenase gene expression in rheumatoid arthritis, we have isolated and characterized genomic clones for rabbit synovial cell collagenase. These clones represent 2 types of collagenase gene, at least 1 of which is transcribed in synovial fibroblasts. By examining the rabbit genome in situ, we provide evidence that there are only 2 different synovial cell collagenase genes found in a haploid genome. Amplification of these genes is not a mechanism for collagenase messenger RNA induction by phorbol esters.
mesh_terms: D000818:Animals; D002999:Clone Cells / Q000201:enzymology; D004247:DNA / Q000235:genetics; D005786:Gene Expression Regulation; D005796:Genes; D005821:Genetic Techniques; D003012:Microbial Collagenase / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D011817:Rabbits; D012091:Repetitive Sequences, Nucleic Acid; D013583:Synovial Membrane / Q000166:cytology / Q000201:enzymology*
chemical_list: D004247:DNA; D003012:Microbial Collagenase