Inhibition of Banzi viral RNA synthesis in BHK-21 cells by Banzi virion matrix protein.
Treatment of BHK-21 cells with purified matrix (M) protein isolated from Banzi virions resulted in decreased production of Banzi virus and in reduced levels of virus-specific positive-strand and negative-strand RNA. The M protein had no effect on the uncoating of the viral genome. This inhibition was virus-specific since the replication of unrelated togaviruses in BHK-21 cells was not affected. Purified M protein inhibited the in vitro activity of the Banzi viral RNA polymerase; prior treatment of the M protein with either trypsin or antiviral serum blocked this inhibition.
D000818:Animals; D002460:Cell Line; D002472:Cell Transformation, Viral; D006224:Cricetinae; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D005416:Flavivirus / Q000201:enzymology / Q000235:genetics*; D006706:Homeostasis; D007668:Kidney; D007700:Kinetics; D012367:RNA, Viral / Q000302:isolation & purification; D013045:Species Specificity; D014158:Transcription, Genetic; D014763:Viral Matrix Proteins; D014764:Viral Proteins / Q000502:physiology*
D012367:RNA, Viral; D014763:Viral Matrix Proteins; D014764:Viral Proteins; D012321:DNA-Directed RNA Polymerases