Measurement of the proton motive force in Rhizobium meliloti with the Escherichia coli lacY gene product.
An Escherichia coli lac operon constitutive for lacY was subcloned into the EcoRI site of a wide-host-range plasmid of the Q incompatibility group, and the resulting recombinant plasmid was introduced into Tn5-generated Lac- mutants of Rhizobium meliloti. The R. meliloti transconjugants accumulated lactose about 1,000-fold, equivalent to a proton motive force of -170 to -180 mV, not significantly different from the values calculated from the distributions of weak acids and lipophilic cations.
D003001:Cloning, Molecular; D003227:Conjugation, Genetic; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000235:genetics; D006863:Hydrogen-Ion Concentration; D007763:Lac Operon; D007785:Lactose / Q000378:metabolism*; D008564:Membrane Potentials; D010957:Plasmids; D011522:Protons; D012231:Rhizobium / Q000235:genetics / Q000378:metabolism*
D004274:DNA, Recombinant; D011522:Protons; D007785:Lactose