Effect of the mobile phase composition on the retention behaviour of diphenylsilica pre-coated plates.
The retention of some rifamycins and steroids on diphenyl bonded pre-coated silica gel plates, in relation to the mobile phase used, was examined by thin-layer chromatography. Neat organic solvents, non-aqueous and aqueous binary mixtures were tested as eluents. By comparison of retention data for rifamycins and steroids, respectively, under non-aqueous and aqueous conditions, a dual retention mechanism on this diphenyl phase was found. Interactions with the residual silanol groups seemed to prevail when employing as mobile phase the more lipophilic solvents tested, such as chloroform or dichloromethane, whereas interactions with the aryl groups of the bonded phase prevailed when using high polarity alcohols or aqueous mixtures. As a consequence, by changing the mobile phase, a large variation in selectivity with a concomitant change in retention order of the test compounds was observed.
D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D012294:Rifamycins / Q000032:analysis*; D012822:Silicon Dioxide; D013256:Steroids / Q000032:analysis*
D012294:Rifamycins; D013256:Steroids; D012822:Silicon Dioxide