Structure of bovine papillomavirus type 1 DNA in a transformed mouse cell line.
Linearized bovine papillomavirus type 1 (BPV-1) DNA was introduced into mouse C127 cells, where it recircularized and replicated as an intact monomeric, extrachromosomal circular form in the resulting transformants. These cells contained a mixture of complex high molecular weight forms that were converted to a linear form of approximately BPV-1 size upon digestion with an enzyme that cuts once within the BPV-1 genome. Further analysis of one of these cell lines revealed that these high molecular weight forms consisted of two components. One was detected on agarose gels as a diffuse smear of slow-migrating material representing linear forms that were tightly associated with host chromosomes, probably by integration. The second component was composed of discrete-sized oligomeric open and supercoiled extrachromosomal circular forms of up to approximately 48 X 10(3) base-pairs (6 tandemly linked BPV-1 genomes) in size. No catenated (interlocked) forms could be detected.
D000818:Animals; D001345:Autoradiography; D052688:Bovine papillomavirus 1 / Q000235:genetics* / Q000378:metabolism; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D004279:DNA, Viral / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D005092:Exonucleases / Q000378:metabolism; D051379:Mice; D008938:Mitosis; D009693:Nucleic Acid Hybridization; D027383:Papillomaviridae / Q000235:genetics*; D014170:Transformation, Genetic
D004279:DNA, Viral; D005092:Exonucleases