Changes of surface glycoproteins after retinoic acid-dibutyryl cAMP-induced differentiation of teratocarcinoma stem cells.
Retinoic acid induces differentiation of embryonal carcinoma F9 cells into parietal endoderm. The surface proteins of F9 cells from induced and control cultures were labeled with the 125I-lactoperoxidase system and analyzed by two-dimensional gel electrophoresis. Their quantitative comparison has shown an 11-fold increase of protein p220 of apparent MW 220,000 and isoelectric point 5.6. Among other enhanced surface proteins, 3.5-fold increases of p50, p45, and p40 of MW 50,000-40,000 and isoelectric point 5.1-5.3 were observed. Simultaneously another surface protein, p70 of MW 70,000 and isoelectric point 6.1-6.3, disappeared. The quantitative changes of surface proteins produced after treatment with retinoic acid were enhanced in the presence of dibutyryl cAMP. Analysis of lectin-binding proteins demonstrated that increasing proteins p220, p50, p45, and p40 have an affinity for concanavalin A, whereas p70, which decreases, has an affinity for wheat germ agglutinin. Antibodies raised against p70 from undifferentiated cells have shown a specific immunoreaction with p220 from differentiated cells and also with the subunit B of purified laminin. The electrophoretic mobilities of p220 and of the B subunit of laminin are similar. It is suggested that p70, p220, and laminin B subunit share structural homology.
D000818:Animals; D003994:Bucladesine / Q000494:pharmacology*; D002454:Cell Differentiation; D002460:Cell Line; D003208:Concanavalin A / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D054278:Embryonal Carcinoma Stem Cells; D006023:Glycoproteins / Q000032:analysis*; D007158:Immunologic Techniques; D007797:Laminin / Q000032:analysis; D037102:Lectins / Q000378:metabolism; D014411:Neoplastic Stem Cells / Q000166:cytology*; D011975:Receptors, Mitogen / Q000032:analysis; D013499:Surface Properties; D013724:Teratoma / Q000648:ultrastructure*; D014212:Tretinoin / Q000494:pharmacology*; D014909:Wheat Germ Agglutinins
D006023:Glycoproteins; D007797:Laminin; D037102:Lectins; D011975:Receptors, Mitogen; D014909:Wheat Germ Agglutinins; D003208:Concanavalin A; D014212:Tretinoin; D003994:Bucladesine