Immobilization of chicken liver fructose 1,6-bisphosphatase on CNBr-activated Sepharose.
Chicken liver fructose 1,6-bisphosphatase is readily immobilized on CNBr-activated Sepharose. The immobilization alters some enzymatic properties. They include broader pH activity curve, loss of activation by K+ or NH+4, increased resistance to inactivation by trypsin, decreased sensitivity to AMP inhibition, and loss of cooperative interaction among AMP-binding sites. The immobilized enzyme retains about 38% or 19% of the specific activity of the native enzyme when the activity is measured in the absence or presence of K+, respectively.
D000249:Adenosine Monophosphate / Q000494:pharmacology; D000641:Ammonia / Q000494:pharmacology; D000818:Animals; D002645:Chickens; D003488:Cyanogen Bromide; D004800:Enzymes, Immobilized / Q000378:metabolism*; D006597:Fructose-Bisphosphatase / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008099:Liver / Q000201:enzymology*; D011188:Potassium / Q000494:pharmacology; D012685:Sepharose; D014357:Trypsin / Q000378:metabolism
D004800:Enzymes, Immobilized; D000249:Adenosine Monophosphate; D000641:Ammonia; D012685:Sepharose; D006597:Fructose-Bisphosphatase; D014357:Trypsin; D003488:Cyanogen Bromide; D011188:Potassium