Mu-type calcium-activated neutral protease in the rat peripheral nerve.
Previously we reported results of an incubation experiment with neurofilaments that supported the existence of a mu-type of Ca2+-activated neutral protease in the rat peripheral nerve; it was active with microM order Ca2+ (mu-CANP). This time, we partially purified the mu-CANP from a crude CANP fraction of rat peripheral nerve by using a DE52 column and a Phenyl-Sepharose column followed again by DE52 column chromatography. The presence of mu-CANP was verified by an immunoblotting technique. The mu-CANP degraded the neurofilament triplet as previously reported; i.e., among the neurofilament triplet, the 160K component was most sensitive, and in the order of the 160K, 68K, and 200K components, respectively.
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002154:Calpain / Q000145:classification / Q000302:isolation & purification* / Q000378:metabolism; D005591:Chemical Fractionation; D002848:Chromatography, DEAE-Cellulose; D003101:Collodion; D004591:Electrophoresis, Polyacrylamide Gel; D007382:Intermediate Filaments / Q000201:enzymology; D008970:Molecular Weight; D010525:Peripheral Nerves / Q000201:enzymology*; D051381:Rats
D003101:Collodion; D002154:Calpain; D002118:Calcium