Cyclic AMP increases incorporation of exogenous fatty acids into triacylglycerols in hamster fibroblasts.
Incorporation of various exogenous saturated or unsaturated [14C]labeled fatty acids (palmitic, stearic, oleic, linoleic and arachidonic) into triacylglycerols by hamster fibroblasts was markedly enhanced (two- to fourfold) in the presence of theophylline or dibutyryl cyclic adenosine monophosphate (dbcAMP). This effect was observed for short-term (1-6 hr) as well as long-term (15-24 hr) preincubation with dbcAMP. In the presence of sodium fluoride, a phosphoprotein phosphatase inhibitor, measurement of diacylglycerol acyltransferase (DGAT) activity in cells pretreated with dbcAMP pointed out a marked increase (3 X) in specific activity. The results suggest that DGAT activity in fibroblasts could be activated by a cAMP-dependent phosphorylation process.
D000217:Acyltransferases / Q000378:metabolism*; D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism; D003994:Bucladesine / Q000494:pharmacology*; D002250:Carbon Radioisotopes; D002478:Cells, Cultured; D006224:Cricetinae; D000242:Cyclic AMP / Q000378:metabolism*; D051048:Diacylglycerol O-Acyltransferase; D005230:Fatty Acids, Nonesterified / Q000378:metabolism*; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D007700:Kinetics; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000378:metabolism; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000378:metabolism; D013229:Stearic Acids / Q000378:metabolism; D013806:Theophylline / Q000494:pharmacology*; D014280:Triglycerides / Q000096:biosynthesis*
D001095:Arachidonic Acids; D002250:Carbon Radioisotopes; D005230:Fatty Acids, Nonesterified; D008041:Linoleic Acids; D010169:Palmitic Acids; D013229:Stearic Acids; D014280:Triglycerides; D016718:Arachidonic Acid; D019308:Palmitic Acid; C031183:stearic acid; D003994:Bucladesine; D019787:Linoleic Acid; D013806:Theophylline; D000242:Cyclic AMP; D000217:Acyltransferases; D051048:Diacylglycerol O-Acyltransferase