Establishment of cell lines derived from ataxia telangiectasia and xeroderma pigmentosum patients with high radiation sensitivity.
Four human fibroblast cell lines, three of which were derived from a patient with ataxia telangiectasia and the other from a patient with xeroderma pigmentosum, were established after transfection with cloned SV40 DNA. These 4 cell lines showed some phenotypes characteristic of neoplastically transformed cells, and had a human karyotype with heteromorphisms identical to those of the parental fibroblasts. Their sensitivity to the cytotoxic effects of gamma-rays or ultraviolet irradiation was as high as those of their parental fibroblasts.
D001260:Ataxia Telangiectasia / Q000235:genetics / Q000473:pathology*; D002460:Cell Line; D002470:Cell Survival / Q000528:radiation effects; D002871:Chromosome Banding; D004260:DNA Repair; D004279:DNA, Viral; D006801:Humans; D013539:Simian virus 40; D014162:Transfection; D014466:Ultraviolet Rays; D014983:Xeroderma Pigmentosum / Q000235:genetics / Q000473:pathology*
D004279:DNA, Viral