title: The metH gene from Salmonella typhimurium LT2: cloning and initial characterization.
abstract: A 19-kb EcoRI DNA fragment carrying the metH gene of Salmonella typhimurium LT2 was cloned into plasmid vector pACYC184 and propagated in Escherichia coli K-12. The size of the metH gene product was observed to be approx. 120 kDa, as determined by SDS-polyacrylamide gel analysis of plasmid-specific polypeptides synthesized in a minicell system. The direction of transcription of the metH gene relative to the cloned fragment was determined, and the positions of translation initiation and termination were estimated.
mesh_terms: D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D005796:Genes; D005798:Genes, Bacterial; D050904:Homocysteine S-Methyltransferase; D008715:Methionine / Q000096:biosynthesis*; D008780:Methyltransferases / Q000235:genetics*; D010957:Plasmids; D012486:Salmonella typhimurium / Q000201:enzymology / Q000235:genetics*; D013045:Species Specificity; D014158:Transcription, Genetic; D014805:Vitamin B 12 / Q000494:pharmacology
chemical_list: D008715:Methionine; D008780:Methyltransferases; D050904:Homocysteine S-Methyltransferase; D004262:DNA Restriction Enzymes; D014805:Vitamin B 12