Type 1 human T-cell leukemia virus small envelope protein expressed in mouse cells by using a bovine papilloma virus-derived shuttle vector.
In an attempt to express the small (transmembrane) envelope protein p21e of type 1 human T-cell leukemia (lymphotrophic) virus (HTLV-1) exclusive of other viral gene products, we have constructed a recombinant plasmid clone (pMBE-1) in a bovine papillomavirus-derived mammalian expression vector. Mouse C127 cells transfected with the pMBE-1 plasmid expressed the introduced HTLV-1 viral gene(s) as demonstrated by Northern blot and indirect immunofluorescence with natural human antisera. The transfected mouse cells were injected into BALB/c mice, and a monoclonal antibody was recovered which specifically recognizes a 21-kilodalton protein present in HTLV-1 virions, indicating that the pMBE-1 plasmid encodes the small envelope protein.
D000818:Animals; D052688:Bovine papillomavirus 1 / Q000235:genetics*; D002460:Cell Line; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D017977:Deltaretrovirus / Q000235:genetics*; D005455:Fluorescent Antibody Technique; D005796:Genes; D005814:Genes, Viral; D005822:Genetic Vectors; D051379:Mice; D008970:Molecular Weight; D027383:Papillomaviridae / Q000235:genetics*; D014759:Viral Envelope Proteins / Q000032:analysis / Q000235:genetics*
D014759:Viral Envelope Proteins; D004262:DNA Restriction Enzymes