Identification of bovine herpesvirus-1 polypeptides involved in serum neutralization.
Bovine herpesvirus (infectious bovine rhinotracheitis virus)-infected cell antigens were solubilized with Nonidet P-40. The crude antigen extract was separated by reaction with bovine hyperimmune serum in line immunoelectrophoresis; individual immunoprecipitates were used to immunize rabbits. Rabbit sera possessing serum neutralizing activity were analyzed by reaction with crude antigen extract in immunoprecipitation sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot. Four virus-specified glycopeptides, with molecular weights of 69-75K, 77-81K, 82-92K and 108-115K, appeared to be involved in inducing serum neutralizing antibody.
D000818:Animals; D000956:Antigens, Viral / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D006020:Glycopeptides / Q000276:immunology*; D007242:Herpesvirus 1, Bovine / Q000276:immunology*; D007122:Immunoelectrophoresis; D007163:Immunosorbent Techniques; D009500:Neutralization Tests; D011817:Rabbits
D000956:Antigens, Viral; D006020:Glycopeptides