Isolation of a mutation resulting in constitutive synthesis of L-fucose catabolic enzymes.
A ribitol-positive transductant of Escherichia coli K-12, JM2112, was used to facilitate the isolation and identification of mutations affecting the L-fucose catabolic pathway. Analysis of L-fucose-negative mutants of JM2112 enabled us to confirm that L-fucose-1-phosphate is the apparent inducer of the fucose catabolic enzymes. Plating of an L-fuculokinase-negative mutant of JM2112 on D-arabinose yielded an isolate containing a second fucose mutation which resulted in the constitutive synthesis of L-fucose permease, isomerase, and kinase. This constitutive mutation differs from the constitutive mutation described by Chen et al. (J. Bacteriol. 159:725-729, 1984) in that it is tightly linked to the fucose genes and appears to be located in the gene believed to code for the positive activator of the L-fucose genes.
D019747:Aldose-Ketose Isomerases; D001089:Arabinose / Q000378:metabolism; D002238:Carbohydrate Epimerases / Q000096:biosynthesis* / Q000235:genetics; D004790:Enzyme Induction; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification; D029968:Escherichia coli Proteins; D005643:Fucose / Q000378:metabolism*; D005798:Genes, Bacterial; D005809:Genes, Regulator; D006600:Hexosephosphates / Q000494:pharmacology; D026901:Membrane Transport Proteins / Q000096:biosynthesis* / Q000235:genetics; D009154:Mutation; D010770:Phosphotransferases / Q000096:biosynthesis* / Q000235:genetics; D017853:Phosphotransferases (Alcohol Group Acceptor); D027981:Symporters
D029968:Escherichia coli Proteins; C088688:FucP protein, E coli; D006600:Hexosephosphates; D026901:Membrane Transport Proteins; D027981:Symporters; C048089:fuculose 1-phosphate; D005643:Fucose; D001089:Arabinose; D010770:Phosphotransferases; D017853:Phosphotransferases (Alcohol Group Acceptor); C020153:fucokinase; D002238:Carbohydrate Epimerases; D019747:Aldose-Ketose Isomerases; C019714:D-arabinose isomerase