High efficiency gene transfer into murine T cell clones using a retroviral vector.
To establish a gene transfer and expression system for murine T cell clones, we have introduced the neomycin phosphotransferase gene encoding resistance to the neomycin analogue, G418, into non-neoplastic inducer T cell clones by using a replication-defective retroviral vector. This method allowed highly efficient gene transfer (20 to 40%) into two inducer T cell clones. The level of viral RNA expression in G418r T cells was 0.1% of poly(A)+ RNA. The infected G418r cells retained physiologic responsiveness to specific antigen as judged by antigen-specific proliferation and production of IL 3.
D000818:Animals; D002472:Cell Transformation, Viral; D002999:Clone Cells / Q000378:metabolism; D005786:Gene Expression Regulation; D005822:Genetic Vectors; D008213:Lymphocyte Activation; D051379:Mice; D009693:Nucleic Acid Hybridization; D009053:Sarcoma Viruses, Murine / Q000235:genetics*; D012513:Sarcoma, Experimental / Q000235:genetics / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D014162:Transfection