Cloning of the STA2 and SGA genes encoding glucoamylases in yeasts and regulation of their expression by the STA10 gene of Saccharomyces cerevisiae.
The Saccharomyces STA2 and SGA genes, encoding the extracellular and intracellular sporulation-specific glucoamylase respectively, have been cloned and their transcription and regulation studied. The STA2 gene differs from the SGA gene in that it contains an extra piece of DNA, which encodes the domain for exportation of the extracellular glucoamylase. The STA2 gene produces a single 2.85 kb transcript. Transcription of the SGA gene is initiated from two different sites, yielding two transcripts of 1.95 and 2.40 kb. Transcription of both STA2 and SGA genes is repressed by the STA10 gene of Saccharomyces cerevisiae.
D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004720:Endonucleases; D005786:Gene Expression Regulation; D005800:Genes, Fungal; D005809:Genes, Regulator; D005087:Glucan 1,4-alpha-Glucosidase / Q000235:genetics*; D005959:Glucosidases / Q000235:genetics*; D010957:Plasmids; D012333:RNA, Messenger / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D015719:Single-Strand Specific DNA and RNA Endonucleases; D014158:Transcription, Genetic
D012333:RNA, Messenger; D004720:Endonucleases; D004262:DNA Restriction Enzymes; D015719:Single-Strand Specific DNA and RNA Endonucleases; D005959:Glucosidases; D005087:Glucan 1,4-alpha-Glucosidase