Genetic and molecular analysis of aroL, the gene for shikimate kinase II in Escherichia coli K-12.
The gene aroL in Escherichia coli K-12, specifying shikimate kinase II, was contransduced with proC at a frequency of 99%. The gene order is lac proC aroL. A 2.7-kilobase BamHI fragment containing aroL+ was cloned into pBR322. This plasmid conferred highly elevated levels of shikimate kinase synthesis which were subject to repression control by tyrR. The aroL gene was localized within a 730-base-pair region by both subcloning and insertional mutagenesis with Tn1000. A second gene, designated aroM and encoding a protein of molecular weight 26,000, is cotranscribed with aroL. Transcription proceeds in the order aroL aroM in a clockwise direction on the chromosome. The function of aroM remains unknown.
D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005798:Genes, Bacterial; D009876:Operon; D010770:Phosphotransferases / Q000235:genetics*; D017853:Phosphotransferases (Alcohol Group Acceptor); D010957:Plasmids; D014158:Transcription, Genetic; D014161:Transduction, Genetic
D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D010770:Phosphotransferases; D017853:Phosphotransferases (Alcohol Group Acceptor); C022642:shikimate kinase