title: Cloning and localization of the Bacillus subtilis chromosome replication terminus, terC.
abstract: A 10.9-kb segment of the Bacillus subtilis 168 chromosome has been cloned in an Escherichia coli plasmid and shown to contain terC (the replication terminus of the chromosome). The terC-containing portion of this plasmid has been subcloned within each of two overlapping fragments of DNA, 1.75 and 1.95 kb, again in E. coli plasmids. These have afforded a more precise definition of the location of terC in the B. subtilis chromosome and provided material for a detailed analysis of the structure and functioning of this site.
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics*; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004261:DNA Replication; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000096:biosynthesis / Q000235:genetics*; D010957:Plasmids
chemical_list: D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes