title: Transfection of Corynebacterium lilium protoplasts.
abstract: A protoplast transfection system has been developed for a lysine-producing bacterium, Corynebacterium lilium, using the DNA of phage CL31. Phage CL31 is lytic and specific to C. lilium and has a genome of approximately 48 kb. The transfection procedure involves a polyethylene-glycol-mediated introduction of the DNA into lysozyme-treated cells and has a maximum efficiency of 3 X 10(4) transfectants per microgram DNA.
mesh_terms: D001435:Bacteriophages / Q000235:genetics*; D003352:Corynebacterium / Q000235:genetics*; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000235:genetics; D011523:Protoplasts; D014162:Transfection
chemical_list: D004279:DNA, Viral; D004262:DNA Restriction Enzymes