Cooperation of mitogenic growth factors with polyoma virus middle T antigen in transformation of secondary cultured rat cells.
Platelet derived growth factor cooperated with middle T antigen in inducing growth in agarose medium of secondary cultured rat embryo cells transfected with a polyoma virus middle T antigen cDNA clone. In contrast, epidermal growth factor and a conditioned medium containing transforming growth factor did not stimulate the colony-forming efficiency of such cells in the agarose medium.
D000818:Animals; D000952:Antigens, Polyomavirus Transforming; D000957:Antigens, Viral, Tumor / Q000494:pharmacology*; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D005260:Female; D005347:Fibroblasts; D009856:Oncogene Proteins, Viral / Q000494:pharmacology*; D010455:Peptides / Q000494:pharmacology*; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology*; D011120:Polyomavirus / Q000235:genetics; D011247:Pregnancy; D051381:Rats; D011916:Rats, Inbred F344; D012685:Sepharose; D014162:Transfection; D015291:Transforming Growth Factors
D000952:Antigens, Polyomavirus Transforming; D000957:Antigens, Viral, Tumor; D009856:Oncogene Proteins, Viral; D010455:Peptides; D010982:Platelet-Derived Growth Factor; D004815:Epidermal Growth Factor; D015291:Transforming Growth Factors; D012685:Sepharose