Ozonolysis of supercoiled pBR322 DNA resulting in strand scission to open circular DNA.
Treatment of supercoiled pBR322 DNA with ozone resulted in the conversion of closed circular DNA to open circular DNA. Restriction analysis of the resulting open circular DNA showed that ozonolysis in the absence of salt caused single strand cleavage at specific sites.
D055598:Chemical Phenomena; D002621:Chemistry; D002874:Chromosome Mapping; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial; D004270:DNA, Circular; D004278:DNA, Superhelical; D006834:Hydrazines; D010126:Ozone; D010957:Plasmids
D004269:DNA, Bacterial; D004270:DNA, Circular; D004278:DNA, Superhelical; D006834:Hydrazines; C029424:hydrazine; D010126:Ozone; D004262:DNA Restriction Enzymes