Isolation of glycoproteins of parainfluenza I (Sendai) by Helix pomatia Lectin column.
Glycoproteins of Sendai virus were successfully isolated on a column of Helix pomatia Lectin-Sepharose 6MB following solubilization with Nonidet P-40. This technique can be used as a preparative step for the study of viral glycoproteins.
D002845:Chromatography; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000302:isolation & purification*; D006389:Hemagglutinins, Viral / Q000302:isolation & purification; D037102:Lectins; D017830:Octoxynol; D010222:Parainfluenza Virus 1, Human / Q000032:analysis*; D011092:Polyethylene Glycols; D014764:Viral Proteins / Q000302:isolation & purification*
D006023:Glycoproteins; C038095:Helix lectin; D006389:Hemagglutinins, Viral; D037102:Lectins; D014764:Viral Proteins; D011092:Polyethylene Glycols; D017830:Octoxynol; C010615:Nonidet P-40