Presentation of Candida albicans and purified protein derivative soluble antigens by Epstein-Barr virus-transformed human lymphoblastoid B-cell lines.
Cells other than the macrophage can function as antigen-presenting cells (APCs). These class II-bearing accessory cells include dendritic cells, epidermal Langerhans cells, B cells, murine B-cell tumors, and human Epstein-Barr virus-transformed lymphoblastoid cell lines (EBV-LCL). We investigated the ability of EBV-LCL to present two soluble antigens, Candida albicans and purified protein derivative of tuberculin (PPD). The EBV-LCL derived from B cells of two different individuals can present both antigens to bulk cultures of autologous antigen-primed peripheral blood lymphocytes. The responses of PPD-reactive T-cell clones were weaker to PPD when presented by EBV-LCL than by PBL-APCs, with some clones responding only to PPD presented by PBL-APCs. This suggests that EBV-LCL are not equivalent to PBL monocytes in APC function, and that expression of class II major histocompatibility complex antigen is not sufficient in enabling antigen-presenting capability.
D000938:Antigen-Presenting Cells / Q000276:immunology*; D000946:Antigens, Fungal / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology*; D002176:Candida albicans / Q000276:immunology; D002460:Cell Line; D002472:Cell Transformation, Viral; D004854:Herpesvirus 4, Human / Q000502:physiology; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D006801:Humans; D013601:T-Lymphocytes / Q000276:immunology; D014373:Tuberculin / Q000276:immunology*
D000946:Antigens, Fungal; D000949:Histocompatibility Antigens Class II; D014373:Tuberculin