Strategies in neuropeptide receptor binding research.
Strategies and general approaches used in neuropeptide receptor binding assays are described. Special attention is given to the nature of the ligand, its physical and chemical stability and the demonstration of an appropriate ligand selectivity pattern. Examples are given to illustrate critical aspects of neuropeptide receptor binding assays. Strong correlation between binding and bioassay data is also stressed.
D000818:Animals; D001681:Biological Assay; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004355:Drug Stability; D006168:Guinea Pigs; D008024:Ligands; D051379:Mice; D009420:Nervous System / Q000032:analysis*; D010455:Peptides / Q000378:metabolism*; D011817:Rabbits; D011869:Radioligand Assay / Q000379:methods*; D051381:Rats; D011956:Receptors, Cell Surface / Q000032:analysis*
D008024:Ligands; D010455:Peptides; D011956:Receptors, Cell Surface