The immunoglobulin heavy-chain B-lymphocyte enhancer efficiently stimulates transcription in non-lymphoid cells.
The mouse immunoglobulin heavy-chain (IgH) B-lymphocyte enhancer stimulates transcription from heterologous promoters 20- to 40-fold when transfected into several non-lymphoid cell lines. Stimulation in B-lymphocyte melanoma cell-lines is only about 5--10 times better. A central sequence is equally active in both cell types, whilst flanking sequences, on either side of the common enhancer sequences, specifically stimulate transcription in myeloma cells. These results suggest that there are factors in non-lymphoid cells that can interact with the IgH enhancer to stimulate transcription.
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D002460:Cell Line; D002478:Cells, Cultured; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant / Q000378:metabolism; D004742:Enhancer Elements, Genetic; D005796:Genes; D005809:Genes, Regulator; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D051379:Mice; D010957:Plasmids; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic
D004274:DNA, Recombinant; D007143:Immunoglobulin Heavy Chains; D004262:DNA Restriction Enzymes