title: A new restriction endonuclease from Spirulina platensis.
abstract: Three restriction endonucleases, Sp1I, Sp1II and Sp1III have been purified partially from Spirulina platensis subspecies siamese and named. Sp1I cleaves bacteriophage lambda DNA at one site, phi X 174 RF DNA at two sites, but does not cleave pBR322 DNA. This enzyme recognizes the sequence 5'CGTACG3' 3'GCATCG5' and cuts the site indicated by the arrows. Sp1II is an isoschizomer of Tth111I and Sp1III is an isoschizomer of HaeIII.
mesh_terms: D001483:Base Sequence; D000458:Cyanobacteria / Q000201:enzymology*; D004262:DNA Restriction Enzymes / Q000302:isolation & purification* / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific; D013379:Substrate Specificity
chemical_list: D004262:DNA Restriction Enzymes; C048420:endodeoxyribonuclease SplI; C048421:endodeoxyribonuclease Tth111I; D015252:Deoxyribonucleases, Type II Site-Specific; C024403:GGCC-specific type II deoxyribonucleases