Structure of the c-Ki-ras gene in a rat fibrosarcoma induced by 1,8-dinitropyrene.
Restriction enzyme maps were made of the region around exons 1 and 2 of activated c-Ki-ras of a fibrosarcoma (1,8-DNP2) induced in a rat by 1,8-dinitropyrene. Nucleotide sequence analysis revealed that activated c-Ki-ras shows a G----T transversion in codon 12 and consequently encodes cysteine instead of glycine in normal rat c-Ki-ras.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002273:Carcinogens / Q000494:pharmacology*; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D004262:DNA Restriction Enzymes; D005354:Fibrosarcoma / Q000235:genetics*; D051379:Mice; D008815:Mice, Inbred Strains; D009857:Oncogenes / Q000187:drug effects*; D011721:Pyrenes / Q000494:pharmacology*
D002273:Carcinogens; D011721:Pyrenes; C033310:1,8-dinitropyrene; D004262:DNA Restriction Enzymes