Further studies of the mechanism of the enhancement of NADH oxidation by vanadate.
O2-., whether generated photochemically, or introduced as a solution of KO2 in a nonprotic solvent, caused rapid oxidation of NADH in the presence, but not in the absence of vanadate. Superoxide dismutase inhibited this vanadate-stimulated oxidation of NADH, while catalase had no effect. This NADH oxidation appears to be a free-radical chain reaction whose average chain length was estimated to be 15 in the photochemical system. Vanadate stimulation of NADH oxidation by biological membranes can now be viewed as a sensitive indicator of O2-. production by those membranes.
D005609:Free Radicals; D066298:In Vitro Techniques; D008982:Molybdenum / Q000378:metabolism; D009243:NAD / Q000378:metabolism*; D010084:Oxidation-Reduction; D013481:Superoxides / Q000378:metabolism*; D014638:Vanadates; D014639:Vanadium / Q000378:metabolism*
D005609:Free Radicals; D014639:Vanadium; D009243:NAD; D013481:Superoxides; C044659:molybdate; D014638:Vanadates; D008982:Molybdenum