5S RNA gene specific transcription factor (TFIIIA) changes the linking number of the DNA.
The purified 5S gene specific transcription factor A (TFIIIA), when incubated with the relaxed 5S gene in the presence of topoisomerase I alters the conformation of the DNA, resulting in a change in the linking number. This interaction introduces a change of one bp per TFIIIA binding site at a low concentration of DNA to protein (1:2) which increases to an extent of 0.9 turns (9 bp) per TFIIIA binding site at a higher protein concentration (1:12). These analyses support the notion that the binding of TFIIIA to the 5S DNA introduces a minimal change in the topology of circular DNA molecules.
D000818:Animals; D001482:Base Composition; D001665:Binding Sites; D004247:DNA / Q000378:metabolism*; D004264:DNA Topoisomerases, Type I; D004270:DNA, Circular / Q000378:metabolism; D004274:DNA, Recombinant / Q000032:analysis; D004278:DNA, Superhelical / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology; D005786:Gene Expression Regulation; D007536:Isomerism; D009690:Nucleic Acid Conformation; D010957:Plasmids; D011485:Protein Binding; D035582:Transcription Factor TFIIIA; D014157:Transcription Factors / Q000502:physiology*; D014158:Transcription, Genetic; D014981:Xenopus
D004270:DNA, Circular; D004274:DNA, Recombinant; D004278:DNA, Superhelical; D035582:Transcription Factor TFIIIA; D014157:Transcription Factors; D004247:DNA; D012321:DNA-Directed RNA Polymerases; D004264:DNA Topoisomerases, Type I