Studies on binding of toromycin, an antitumor antibiotic, to DNA.
Toromycin, an antitumor, bactericidal and antiviral compound, was found to bind to DNA in such a way as to interfere with the dissociation of double helix at an elevated temperature. The antibiotic did not introduce strand scission into DNA. Single-strand-specific nuclease S1-susceptibility of negatively supercoiled DNA was not influenced by its binding. The antibiotic was shown to bind to both of the alternating purine-pyrimidine copolymers, poly(dG-dC):poly(dG-dC) and poly(dA-dT):poly(dA-dT). The unique C-glycoside molecule of toromycin interacted with single-stranded DNA, but was found to have no affinity for RNA.
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D000903:Antibiotics, Antineoplastic / Q000378:metabolism*; D010582:Bacteriophage lambda / Q000235:genetics; D004278:DNA, Superhelical / Q000378:metabolism; D004279:DNA, Viral / Q000378:metabolism*; D004587:Electrophoresis, Agar Gel; D004720:Endonucleases / Q000378:metabolism; D006027:Glycosides / Q000378:metabolism*; D006358:Hot Temperature; D011067:Poly dA-dT / Q000378:metabolism; D011089:Polydeoxyribonucleotides / Q000378:metabolism; D015719:Single-Strand Specific DNA and RNA Endonucleases
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D000903:Antibiotics, Antineoplastic; D004278:DNA, Superhelical; D004279:DNA, Viral; D006027:Glycosides; D011089:Polydeoxyribonucleotides; D011067:Poly dA-dT; C019530:poly(dC-dG); C048811:toromycin; D004720:Endonucleases; D015719:Single-Strand Specific DNA and RNA Endonucleases