title: Immunoperoxidase staining for the detection of herpes simplex virus antigen in cervicovaginal smears.
abstract: Destained cervicovaginal smears from eight patients with herpes simplex virus (HSV) infections were stained by means of the peroxidase-antiperoxidase (PAP) technique to demonstrate the presence of the HSV type 2 (HSV-2) antigen. Positive results were obtained in six of the eight cases, with intense staining for the HSV-2-specific antigen throughout the cytoplasm and nuclei of cells having a ground-glass nuclear appearance as well as in multinucleated giant cells. Virus isolation was successfully performed for the HSV-2-positive case that also had a histologically confirmed squamous-cell carcinoma of the cervix. The combined use of cytology and the PAP staining technique was of great value in the demonstration of cervical HSV infections.
mesh_terms: D000328:Adult; D000956:Antigens, Viral / Q000032:analysis; D002294:Carcinoma, Squamous Cell / Q000150:complications / Q000473:pathology; D002467:Cell Nucleus / Q000276:immunology / Q000382:microbiology; D003593:Cytoplasm / Q000382:microbiology; D005260:Female; D006558:Herpes Genitalis / Q000175:diagnosis*; D006801:Humans; D007124:Immunoenzyme Techniques; D008875:Middle Aged; D018139:Simplexvirus / Q000276:immunology; D002577:Uterine Cervical Diseases / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology; D002583:Uterine Cervical Neoplasms / Q000150:complications / Q000473:pathology; D014623:Vaginal Diseases / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology
chemical_list: D000956:Antigens, Viral