Recombinant human granulocyte-macrophage colony-stimulating factor stimulates in vitro mature human neutrophil and eosinophil function, surface receptor expression, and survival.
A purified recombinant human granulocyte-macrophage colony stimulating factor (rH GM-CSF) was a powerful stimulator of mature human eosinophils and neutrophils. The purified rH GM-CSF enhanced the cytotoxic activity of neutrophils and eosinophils against antibody-coated targets, stimulated phagocytosis of serum-opsonized yeast by both cell types in a dose-dependent manner, and stimulated neutrophil-mediated iodination in the presence of zymosan. In addition, rH GM-CSF enhanced N-formylmethionylleucylphenylalanine(FMLP)-stimulated degranulation of Cytochalasin B pretreated neutrophils and FMLP-stimulated superoxide production. In contrast, rH GM-CSF did not promote adherence of granulocytes to endothelial cells or plastic surfaces. rH GM-CSF selectively enhanced the surface expression of granulocyte functional antigens 1 and 2, and the Mo1 antigen. rH GM-CSF induced morphological changes and enhanced the survival of both neutrophils and eosinophils by 6 and 9 h, respectively. These experiments show that granulocyte-macrophage colony stimulating factor can selectively stimulate mature granulocyte function.
D000920:Antibody-Dependent Cell Cytotoxicity; D002448:Cell Adhesion / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003594:Cytoplasmic Granules / Q000648:ultrastructure; D004804:Eosinophils / Q000166:cytology / Q000276:immunology / Q000502:physiology*; D005434:Flow Cytometry; D006801:Humans; D007377:Interleukin-3 / Q000502:physiology*; D009504:Neutrophils / Q000166:cytology / Q000276:immunology / Q000502:physiology*; D010587:Phagocytosis; D011994:Recombinant Proteins / Q000494:pharmacology*; D013481:Superoxides / Q000097:blood
D007377:Interleukin-3; D011994:Recombinant Proteins; D013481:Superoxides