Influence of twenty potentially antiviral substances on in vitro multiplication of hepatitis A virus.
A multiwell tissue culture system was developed to study the influence of various substances on hepatitis A virus (HAV) propagation. A panel of 20 substances of different structure types, each with known effect against at least some viruses, was studied at a concentration of 100 microM. Three substances showed reproducible inhibition. The strongest inhibitor, arabinosylcytosine, also produced cytotoxic changes in cells down to a concentration of 1 microM, and its effect was considered as nonspecific. Amantadine and ribavirin showed a moderate effect at 100 microM. A stronger inhibition was seen at 250 and 500 microM, doses that are toxic and impractical for clinical use. Although no promising candidates for antiviral treatment of hepatitis A have emerged from the present study, the assay model described here would seem useful in the screening of substances with inhibitory effects on HAV.
D000547:Amantadine / Q000494:pharmacology / Q000633:toxicity; D000998:Antiviral Agents / Q000494:pharmacology* / Q000627:therapeutic use / Q000633:toxicity; D002460:Cell Line; D003561:Cytarabine / Q000494:pharmacology / Q000633:toxicity; D004353:Drug Evaluation, Preclinical; D006506:Hepatitis A / Q000188:drug therapy*; D006507:Hepatovirus / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D011863:Radioimmunoassay; D012254:Ribavirin / Q000494:pharmacology / Q000633:toxicity; D014779:Virus Replication / Q000187:drug effects
D000998:Antiviral Agents; D003561:Cytarabine; D012254:Ribavirin; D000547:Amantadine