[Phosphorylation as a method of regulating inorganic pyrophosphatase activity in E. coli. I. Phosphorylation and enzyme activation induced by ATP].
ATP phosphorylates the regulatory center of E. coli inorganic pyrophosphatase with the resultant 1,5-fold increase in the activity of the enzyme. The maximal incorporation of the ATP gamma-group into pyrophosphatase is 3 moles per mole of the protein. Pi likewise phosphorylates the enzyme regulatory center and lowers the pyrophosphatase activity by 10-15%. The ATP- and Pi-mediated phosphorylation processes are interrelated; ATP prevents phosphorylation by Pi and brings about rapid dephosphorylation of Pi-modified protein.
D000255:Adenosine Triphosphate / Q000378:metabolism*; D001665:Binding Sites; D004789:Enzyme Activation; D004926:Escherichia coli / Q000201:enzymology* / Q000378:metabolism; D043564:Inorganic Pyrophosphatase; D010710:Phosphates / Q000378:metabolism; D010766:Phosphorylation; D011755:Pyrophosphatases / Q000378:metabolism*
D010710:Phosphates; D000255:Adenosine Triphosphate; D011755:Pyrophosphatases; D043564:Inorganic Pyrophosphatase
Fosforilirovanie kak sposob reguliatsii aktivnosti neorganicheskoĭ pirofosfatazy E. coli. I. Fosforilirovanie i aktivatsiia fermenta pod deĭstviem ATP.