title: Nucleotide sequence of the chicken 5-aminolevulinate synthase gene.
abstract: 5-Aminolevulinate synthase, the first and rate-controlling enzyme of heme biosynthesis, is regulated in the liver by the end-product heme. To study this negative control mechanism, we have isolated the chicken gene for ALA-synthase and determined the nucleotide sequence. The structural gene is 6.9 kb long and contains 10 exons. The transcriptional start site for ALA-synthase was determined by primer extension analysis. A fragment of 291 bp from the 5' flanking region including 34 bp of the first exon shows promoter activity when introduced upstream of a chicken histone H2B gene and injected into the nuclei of Xenopus laevis oocytes.
mesh_terms: D000624:5-Aminolevulinate Synthetase / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002642:Chick Embryo; D002645:Chickens / Q000235:genetics*; D002874:Chromosome Mapping; D004262:DNA Restriction Enzymes; D005786:Gene Expression Regulation / Q000187:drug effects; D005796:Genes; D008040:Genetic Linkage; D008930:Mitochondria, Liver / Q000201:enzymology; D011401:Promoter Regions, Genetic
chemical_list: D000624:5-Aminolevulinate Synthetase; D004262:DNA Restriction Enzymes