[Insertion of the bacterial gene for dihydrofolate reductase into colony-forming cells of mouse bone marrow].
Introduction of the plasmid containing the methotrexate-resistant (Mtx-r) bacterial gene of dihydrofolate reductase (DHFR) under the control of the early promoter of SV 40 into the donor bone cells of the mouse with subsequent transplantation of the cells into lethally irradiated mice results in the increase in the life span of mice under conditions of methotrexate selection. It is due to the stable transformation of the bone marrow colony-forming cells with the plasmic DNA and the synthesis of the bacterial Mtx-r DHFR in the spleen and bone marrow of the recipient mouse.
D000818:Animals; D001853:Bone Marrow / Q000187:drug effects / Q000201:enzymology; D001854:Bone Marrow Cells; D004247:DNA / Q000235:genetics; D004352:Drug Resistance, Microbial; D005493:Folic Acid Antagonists; D005786:Gene Expression Regulation / Q000187:drug effects; D005798:Genes, Bacterial / Q000187:drug effects; D005821:Genetic Techniques; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology; D008297:Male; D008727:Methotrexate / Q000037:antagonists & inhibitors; D051379:Mice; D008808:Mice, Inbred CBA; D010957:Plasmids; D011401:Promoter Regions, Genetic; D013539:Simian virus 40 / Q000235:genetics; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics*; D014916:Whole-Body Irradiation
D005493:Folic Acid Antagonists; D004247:DNA; D013762:Tetrahydrofolate Dehydrogenase; D008727:Methotrexate
Vvedenie bakterial'nogo gena digidrofolatreduktazy v kolonieobrazuiushchie kletki kostnogo mozga myshi.