Multiple regulation of STE2, a mating-type-specific gene of Saccharomyces cerevisiae.
The Saccharomyces cerevisiae STE2 gene, which is required for pheromone response and conjugation specifically in mating-type a cells, was cloned by complementation of the ste2 mutation. Transcription of STE2 is repressed by the MAT alpha 2 gene product, so that the 1.4-kilobase STE2 RNA is detected only in a or mat alpha 2 strains, not in alpha or a/alpha cells. However, STE2 RNA levels are also increased by the mating pheromone alpha-factor and decreased in strains bearing mutations in the nonspecific STE4 gene. Regulation of STE2 expression in a cells is therefore achieved by several mechanisms.
D001483:Base Sequence; D003227:Conjugation, Genetic; D005786:Gene Expression Regulation; D005800:Genes, Fungal; D049770:Genes, Mating Type, Fungal; D005816:Genetic Complementation Test; D000072235:Mating Factor; D010455:Peptides / Q000502:physiology; D010675:Pheromones / Q000502:physiology; D011956:Receptors, Cell Surface / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014158:Transcription, Genetic
D010455:Peptides; D010675:Pheromones; D011956:Receptors, Cell Surface; D000072235:Mating Factor