Partial characterization and solubilization of receptors for atrial natriuretic factor in rat glomeruli.
Specific receptors for atrial natriuretic factor (ANF) have been identified and solubilized in glomeruli from rat kidney. Radioiodinated synthetic ANF (Arg 101-Tyr 126) bound to a single class of high affinity (Kd 27 +/- 24 pM) sites with a density of 390 +/- 230 fmole/mg protein. The binding was time- and temperature-dependent, saturable and reversible. The ANF-receptor complex was not affected by angiotensin II, ACTH or vasopressin. Solubilization with 10 mM 3-[(3-cholamidopropyl)-dimethylammonio]- 1-propane sulfonate (CHAPS) slightly increased the affinity for ANF (Kd 5.0 +/- 3.3 pM) without affecting the density (250 +/- 110 fmole/mg protein). Similar results were found with 1% Triton X-100. ANF-related peptides interact generally in the same way with non-solubilized and solubilized receptors, indicating a fully preserved specificity of the receptors.
D000324:Adrenocorticotropic Hormone / Q000494:pharmacology; D000804:Angiotensin II / Q000494:pharmacology; D000818:Animals; D009320:Atrial Natriuretic Factor / Q000378:metabolism; D002793:Cholic Acids; D003902:Detergents; D007678:Kidney Glomerulus / Q000032:analysis*; D008565:Membrane Proteins / Q000302:isolation & purification* / Q000378:metabolism; D017830:Octoxynol; D011092:Polyethylene Glycols; D051381:Rats; D017461:Receptors, Atrial Natriuretic Factor; D011956:Receptors, Cell Surface / Q000302:isolation & purification* / Q000378:metabolism; D012995:Solubility; D014667:Vasopressins / Q000494:pharmacology
D002793:Cholic Acids; D003902:Detergents; D008565:Membrane Proteins; D011956:Receptors, Cell Surface; C472610:atrial natriuretic peptide, rat; D014667:Vasopressins; D000804:Angiotensin II; D011092:Polyethylene Glycols; D009320:Atrial Natriuretic Factor; D000324:Adrenocorticotropic Hormone; D017830:Octoxynol; D017461:Receptors, Atrial Natriuretic Factor; C028213:3-((3-cholamidopropyl)dimethylammonium)-1-propanesulfonate