[Immunoenzyme detection of enzootic bovine leukemia viruses (BLV) in cell cultures].
There is a description of the method of immunoenzymic test used for detection of enzootic bovine leukosis virus (BLV) on the cells of foetal lamb spleen (FLS) permanently producing BLV and on the lymphocytes of infected animals. In both cases (in FLS cells and lymphocytes) the presence of BLV was demonstrated by the immunoperoxidase test. Using the defined serums from the animals after experimental and natural infection, BLV was detected by the above-mentioned test in FLS cells in all cases of the use of positive serums with anti-BLV antibodies. The specificity of the antibodies was also demonstrated in this way. The presence of BLV was analogically detected in the lymphocytes of the infected animals where the infection time was precisely defined. The method of immunoperoxidase test on lymphocytes is recommended in indicated cases for the demonstration of the presence of BLV in infected animals.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D002417:Cattle; D002478:Cells, Cultured; D005260:Female; D007124:Immunoenzyme Techniques; D001909:Leukemia Virus, Bovine / Q000254:growth & development / Q000276:immunology*; D008297:Male; D012190:Retroviridae / Q000276:immunology*
D000914:Antibodies, Viral
Imunoenzymatický průkaz viru enzootické leukózy skotu (BLV) v bunĕcných kulturách.