Elimination of Fc receptor binding of human immunoglobulin G in immunofluorescence assays for herpes simplex virus antibodies.
The binding of human immunoglobulin G by Fc receptors in herpes simplex virus (HSV)-infected cells can cause false-positive interpretations in the immunofluorescence test for HSV antibody. When the infected cell smears were treated with 10% glacial acetic acid for 5 min and rinsed in phosphate-buffered saline before the immunofluorescence test was performed, the Fc receptors were completely inactivated, resulting in a reliable method for HSV antibody detection.
D000085:Acetates; D019342:Acetic Acid; D000914:Antibodies, Viral / Q000032:analysis*; D005189:False Positive Reactions; D005455:Fluorescent Antibody Technique; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007074:Immunoglobulin G / Q000276:immunology*; D011961:Receptors, Fc / Q000276:immunology*; D018139:Simplexvirus / Q000276:immunology*
D000085:Acetates; D000914:Antibodies, Viral; D007074:Immunoglobulin G; D011961:Receptors, Fc; D019342:Acetic Acid