Restriction endonuclease analysis of Brucella ovis and other Brucella species.
Brucella ovis DNA was analysed by using 11 different restriction endonucleases. The most clearly resolved DNA fragment patterns were obtained after digestion with the enzyme Hind III. When DNA preparations from 35 strains of B. ovis were digested with this enzyme, the fragment patterns appeared to be identical. The patterns obtained after Hind III digestion of DNA from one strain each of B. abortus, B. canis and B. melitensis were more similar to each other than to the B. ovis pattern.
D000818:Animals; D002002:Brucella / Q000145:classification / Q000235:genetics*; D002003:Brucella abortus / Q000145:classification / Q000235:genetics*; D002006:Brucellosis / Q000382:microbiology / Q000662:veterinary; D002417:Cattle; D004262:DNA Restriction Enzymes / Q000235:genetics; D004269:DNA, Bacterial / Q000032:analysis*; D015247:Deoxyribonuclease HindIII; D014644:Genetic Variation; D008297:Male; D012756:Sheep; D012757:Sheep Diseases / Q000382:microbiology
D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes; D015247:Deoxyribonuclease HindIII