Identification of protein phosphatases dephosphorylating mRNP proteins from cryptobiotic gastrulae of the brine shrimp A. salina.
In the cytosol of A. salina cryptobiotic gastrulae at least five protein phosphatases active on phosphorylase a have been detected by ion exchange chromatography on DEAE-cellulose. Only two of these enzymes (PP-X and PP-Y) are active in mRNP dephosphorylation. Both enzymes are insensitive to inhibitor-1 and -2 and stimulation of enzymatic activity (2.5-fold with PP-X and 6.5-fold with PP-Y) can be accomplished by ethanol treatment of the native enzymes, or freeze-thawing in the presence of 1.7% (v/v) 2-mercaptoethanol. These properties allow PP-X and PP-Y to be classified as type-2A enzymes according to the nomenclature of Cohen. This paper is the first report of protein phosphatases capable of dephosphorylating mRNP proteins.
D000818:Animals; D001156:Artemia / Q000196:embryology / Q000201:enzymology*; D002848:Chromatography, DEAE-Cellulose; D003600:Cytosol / Q000201:enzymology; D000431:Ethanol / Q000494:pharmacology; D005615:Freezing; D005775:Gastrula / Q000201:enzymology; D010749:Phosphoprotein Phosphatases / Q000037:antagonists & inhibitors / Q000302:isolation & purification / Q000378:metabolism*; D010762:Phosphorylase a / Q000378:metabolism; D010766:Phosphorylation; D012261:Ribonucleoproteins / Q000378:metabolism*; D012969:Sodium Fluoride / Q000494:pharmacology
D012261:Ribonucleoproteins; C026452:messenger ribonucleoprotein; D000431:Ethanol; D012969:Sodium Fluoride; D010762:Phosphorylase a; D010749:Phosphoprotein Phosphatases