title: Cellular structure and function of mouse adrenocortical tumor cells Y-1 in the post-treatment state of low Ca2+.
abstract: Under lowered Ca2+ content and in the post-treatment state of low Ca2+, we studied the cellular structure and functioning in mouse adrenocortical tumor cells, Y-1. These cells had been maintained in Ham F12 medium containing 10% fetal calf serum. The Ca2+ present in this complete medium was 0.39 mM. Under a slightly lowered Ca2+ (0.29 mM) produced by EGTA, the cells had many blebs on their surfaces and specific functional activity decreased in steroidogenesis as did ACTH reactivity. In the post-treatment state of a low Ca2+, the cellular surface was covered with many short microvilli and there was greater cellular activity than in the control cells. When the Ca2+ concentration was below 0.17 mM, the cellular structure and functioning were disturbed, and there was no recovery even at the physiological Ca2+ after the removal of EGTA.
mesh_terms: D000306:Adrenal Cortex Neoplasms / Q000378:metabolism* / Q000648:ultrastructure; D000324:Adrenocorticotropic Hormone / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000172:deficiency* / Q000378:metabolism; D002460:Cell Line; D002462:Cell Membrane / Q000648:ultrastructure; D003571:Cytochalasin B / Q000494:pharmacology; D004533:Egtazic Acid / Q000494:pharmacology; D051379:Mice; D008855:Microscopy, Electron, Scanning; D011956:Receptors, Cell Surface / Q000032:analysis; D018043:Receptors, Corticotropin; D013997:Time Factors
chemical_list: D011956:Receptors, Cell Surface; D018043:Receptors, Corticotropin; D003571:Cytochalasin B; D004533:Egtazic Acid; D000324:Adrenocorticotropic Hormone; D002118:Calcium