Transport and intracellular distribution of copper in a human hepatoblastoma cell line, HepG2.
The uptake of radiocopper by HepG2 cells is a saturable, temperature-dependent and cellular energy-independent process with a Vmax of 7.1 +/- 0.2 pmoles min-1 mg protein-1 and an estimated Km of 3.3 +/- 0.5 microM. The rate of copper uptake is reduced at an equimolar concentration of albumin and is unaffected by zinc at a 10-fold molar excess. Approximately 70% of the newly incorporated radiocopper binds to membranes and organelles, while 30% is recovered in the cytosol. The soluble fraction can be resolved into two copper-binding protein peaks. Incubation of HepG2 with nonisotopic copper results in displacement of radiocopper associated with the proteins contained in the lower molecular weight peak. Exposure of the cells to cycloheximide inhibits the incorporation of the isotope into this fraction.
D001692:Biological Transport; D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D002460:Cell Line; D003300:Copper / Q000378:metabolism*; D006801:Humans; D008113:Liver Neoplasms; D011868:Radioisotopes; D012710:Serum Albumin, Bovine; D013347:Subcellular Fractions / Q000378:metabolism; D013696:Temperature; D013997:Time Factors
D011868:Radioisotopes; D012710:Serum Albumin, Bovine; D003300:Copper