title: Inoculation of lambs with ovine adenovirus 5 (Mastadenovirus ovi 5) strain RTS-42.
abstract: Twelve 1-week-old colostrum-deprived lambs inoculated with the RTS-42 strain of Mastadenovirus ovi 5 were killed and necropsied (2 lambs/day) on postinoculation days (PID) 2, 4, 6, 8, 12, and 21. Four noninoculated lambs were killed and necropsied (2/day) on PID 6 and 12. Virus was isolated from nasal secretions and feces on PID 1 to PID 6, from tracheal fluids and lung tissue of lambs necropsied on PID 2, 4, and 6, and from lung tissue from 1 lamb necropsied on PID 8. Virus was not recovered from liver, kidney, or small intestine of inoculated lambs or samples from noninoculated lambs. Serum antibody was first detected on PID 6 in the inoculated lambs. Noninoculated lambs remained seronegative. None of the lambs in the study developed clinical signs of infection although lesions were produced in the respiratory tract.
mesh_terms: D000256:Adenoviridae / Q000302:isolation & purification / Q000472:pathogenicity*; D000257:Adenoviridae Infections / Q000382:microbiology* / Q000473:pathology; D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D002478:Cells, Cultured; D003315:Cornea / Q000196:embryology; D007668:Kidney / Q000196:embryology; D012756:Sheep
chemical_list: D000914:Antibodies, Viral