Subcellular distribution of hydrolases in Naegleria fowleri.
The presence and particle association of various hydrolytic enzymes in Naegleria fowleri has been studied in whole cell extracts of trophozoites in an effort to establish authentic markers for surface membrane and lysosomal components. Evidence from the experiments reported here indicates that in N. fowleri a) acid proteinase, N-acetylglucosaminidase, and acid phosphatase are associated with cytoplasmic granules closely resembling lysosomes; b) 5'-nucleotidase is associated with the surface membrane, probably on the external surface; c) aspartate aminotransferase is associated with mitochondria; d) alpha-D-glucosidase and an aminopeptidase have bimodal distributions, activity being associated with both the surface membrane and lysosomal particles.
D015720:5'-Nucleotidase; D000118:Acetylglucosaminidase / Q000032:analysis / Q000378:metabolism; D000135:Acid Phosphatase / Q000032:analysis / Q000378:metabolism; D000656:Amoeba / Q000201:enzymology* / Q000648:ultrastructure; D000818:Animals; D001219:Aspartate Aminotransferases / Q000032:analysis / Q000378:metabolism; D016282:Aspartic Acid Endopeptidases; D002462:Cell Membrane / Q000201:enzymology; D010450:Endopeptidases / Q000032:analysis / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D006867:Hydrolases / Q000032:analysis* / Q000378:metabolism; D008247:Lysosomes / Q000201:enzymology; D008854:Microscopy, Electron; D008928:Mitochondria / Q000201:enzymology; D009708:Nucleotidases / Q000032:analysis / Q000378:metabolism; D013347:Subcellular Fractions / Q000201:enzymology; D000520:alpha-Glucosidases / Q000032:analysis / Q000378:metabolism
D001219:Aspartate Aminotransferases; D006867:Hydrolases; D009708:Nucleotidases; D000135:Acid Phosphatase; D015720:5'-Nucleotidase; D000520:alpha-Glucosidases; D000118:Acetylglucosaminidase; D010450:Endopeptidases; D016282:Aspartic Acid Endopeptidases