title: Human parainfluenza type 3 virus hemagglutinin-neuraminidase glycoprotein: nucleotide sequence of mRNA and limited amino acid sequence of the purified protein.
abstract: The nucleotide sequence of mRNA for the hemagglutinin-neuraminidase (HN) protein of human parainfluenza type 3 virus obtained from the corresponding cDNA clone had a single long open reading frame encoding a putative protein of 64,254 daltons consisting of 572 amino acids. The deduced protein sequence was confirmed by limited N-terminal amino acid microsequencing of CNBr cleavage fragments of native HN that was purified by immunoprecipitation. The HN protein is moderately hydrophobic and has four potential sites (Asn-X-Ser/Thr) of N-glycosylation in the C-terminal half of the molecule. It is devoid of both the N-terminal signal sequence and the C-terminal membrane anchorage domain characteristic of the hemagglutinin of influenza virus and the fusion (F0) protein of the paramyxoviruses. Instead, it has a single prominent hydrophobic region capable of membrane insertion beginning at 32 residues from the N terminus. This N-terminal membrane insertion is similar to that of influenza virus neuraminidase and the recently reported structures of HN proteins of Sendai virus and simian virus 5.
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D006023:Glycoproteins / Q000235:genetics; D015707:HN Protein; D006389:Hemagglutinins, Viral / Q000235:genetics*; D008970:Molecular Weight; D009439:Neuraminidase / Q000235:genetics*; D010224:Parainfluenza Virus 3, Human / Q000235:genetics*; D010446:Peptide Fragments / Q000032:analysis; D010957:Plasmids; D012333:RNA, Messenger / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D018108:Respirovirus / Q000235:genetics*; D012995:Solubility; D014759:Viral Envelope Proteins / Q000235:genetics*
chemical_list: D006023:Glycoproteins; D015707:HN Protein; D006389:Hemagglutinins, Viral; D010446:Peptide Fragments; D012333:RNA, Messenger; D012367:RNA, Viral; D014759:Viral Envelope Proteins; D009439:Neuraminidase