Validation of an in vivo alkaline elution assay to detect DNA damage in rat testicular cells.
An alkaline elution assay was used to measure DNA damage in the rat testis after in vivo treatment with chemicals. All of the chemicals reported to induce heritable mutations in a mammalian assay (mouse specific locus test) were positive in the rat alkaline elution assay. Chemicals that are negative or inconclusive in the specific locus test did not cause detectable DNA damage in rat testes. DNA damage was detected by alkaline elution after either intraperitoneal or oral administration of chemical mutagens. Data from these validation studies were used to establish criteria to be used for evaluation of alkaline elution results with materials of unknown potential for genotoxic effects in germinal tissue.
D000818:Animals; D002699:Chlorambucil / Q000633:toxicity; D003432:Cross-Linking Reagents; D003520:Cyclophosphamide / Q000633:toxicity; D004247:DNA / Q000235:genetics*; D004121:Dimethyl Sulfoxide / Q000633:toxicity; D008297:Male; D008698:Mesylates / Q000633:toxicity; D008970:Molecular Weight; D009152:Mutagenicity Tests / Q000379:methods*; D009153:Mutagens / Q000633:toxicity; D011344:Procarbazine / Q000633:toxicity; D051381:Rats; D011919:Rats, Inbred Strains; D013737:Testis / Q000187:drug effects; D014265:Triethylenemelamine / Q000633:toxicity
D003432:Cross-Linking Reagents; D008698:Mesylates; D009153:Mutagens; D002699:Chlorambucil; D011344:Procarbazine; D003520:Cyclophosphamide; D004247:DNA; C046861:n-propyl methane sulfonate; D014265:Triethylenemelamine; D004121:Dimethyl Sulfoxide