Restrictive events in the replication of hepatitis A virus in vitro.
Hepatitis A virus was purified from the feces of 2 patients with unrelated, naturally acquired infections and was inoculated into FRhK-4 cells. Analysis of protein synthesis by double-label coelectrophoresis and subtraction allowed the resolution of virus-specific proteins synthesized during infection. In FRhK-4 cells the two strains of virus studied produced markedly different profiles of virus-specified proteins, with an accumulation of high-molecular-weight proteins for strain HM790 relative to strain HM175, suggesting a level of restriction in the processing of the viral polyprotein.
D000954:Antigens, Surface / Q000276:immunology; D002478:Cells, Cultured; D005455:Fluorescent Antibody Technique; D006507:Hepatovirus / Q000254:growth & development* / Q000276:immunology; D006801:Humans; D017955:Poliovirus / Q000254:growth & development; D014764:Viral Proteins / Q000096:biosynthesis* / Q000276:immunology; D014779:Virus Replication
D000954:Antigens, Surface; D014764:Viral Proteins