Metabolic profiling of radioreceptor-assayable opioid peptides in a human pituitary ACTH-secreting tumor.
The profile of endogenous opioid peptides in the peptide-rich fraction obtained from a homogenate of an ACTH-secreting human pituitary tumor is presented. Gradient RP-HPLC is used to separate the mixture into peptide constituents. A preparation of opioid receptors is used in a radioreceptor assay with ethorphine - a relatively non-specific ligand that is used as a screen because it interacts with mu, sigma, and delta receptors - as the HPLC detector to detect a range of peptides that derive from proenkephalin A and POMC.
D000324:Adrenocorticotropic Hormone / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D003429:Cross Reactions; D003480:Cushing Syndrome / Q000378:metabolism; D004723:Endorphins / Q000032:analysis*; D005260:Female; D006801:Humans; D008875:Middle Aged; D010911:Pituitary Neoplasms / Q000032:analysis* / Q000378:metabolism; D011869:Radioligand Assay / Q000379:methods; D001615:beta-Endorphin; D008083:beta-Lipotropin / Q000032:analysis
D004723:Endorphins; D001615:beta-Endorphin; D000324:Adrenocorticotropic Hormone; D008083:beta-Lipotropin