title: Detection of human papillomavirus DNA in genital warts, cervical dysplasias and neoplasias.
abstract: Biopsies from human genital lesions in Japan (108 samples), including condyloma acuminata, squamous metaplasia, dysplasia, and cervical cancer, were screened for the presence of human papillomavirus (HPV) 6-, 11-, 16-, and 18-related DNAs by spot hybridization and Southern blot hybridization under stringent conditions. By spot hybridization, HPV 6/11-related DNA was found in 92.9% (13/14) of condyloma acuminata and in 6.7% (1/15) of cervical cancer biopsies. HPV 16/18-related DNA was found in 37.7% (5/14) of cervical cancer biopsies and was exclusively associated with invasive squamous cell cancer, 66.7% (8/12), and with cervical carcinoma in situ, 50% (9/18). Some sample DNAs were further characterized by restriction enzyme digestion followed by Southern blot analysis, and we confirmed the presence of HPV-specific DNA fragments and mixed infection with both HPV 6/11- and HPV 16/18-related HPVs in one lesion.
mesh_terms: D000328:Adult; D000368:Aged; D002278:Carcinoma in Situ / Q000382:microbiology; D002294:Carcinoma, Squamous Cell / Q000382:microbiology; D003218:Condylomata Acuminata / Q000382:microbiology*; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D006801:Humans; D008679:Metaplasia / Q000382:microbiology; D008875:Middle Aged; D009693:Nucleic Acid Hybridization; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D002578:Uterine Cervical Dysplasia / Q000382:microbiology* / Q000473:pathology; D002583:Uterine Cervical Neoplasms / Q000382:microbiology* / Q000473:pathology
chemical_list: D004279:DNA, Viral