Characterization of foot-and-mouth disease virus gene products with antisera against bacterially synthesized fusion proteins.
Defined segments of the cloned foot-and-mouth disease virus genome corresponding to all parts of the coding region were expressed in Escherichia coli as fusions to the N-terminal part of the MS2-polymerase gene under the control of the inducible lambda PL promoter. All constructs yielded large amounts of proteins, which were purified and used to raise sequence-specific antisera in rabbits. These antisera were used to identify the corresponding viral gene products in 35S-labeled extracts from foot-and-mouth disease virus gene products in the nucleotide sequence, to identify precursor-product relationships, and to detect several foot-and-mouth disease virus gene products not previously identified in vivo or in vitro.
D000818:Animals; D005537:Aphthovirus / Q000032:analysis* / Q000235:genetics; D001426:Bacterial Proteins / Q000032:analysis* / Q000096:biosynthesis / Q000276:immunology; D002478:Cells, Cultured; D011232:Chemical Precipitation; D006224:Cricetinae; D004926:Escherichia coli / Q000235:genetics; D005786:Gene Expression Regulation; D007106:Immune Sera / Q000276:immunology*; D007668:Kidney; D010957:Plasmids; D011817:Rabbits; D014764:Viral Proteins / Q000032:analysis* / Q000096:biosynthesis / Q000276:immunology
D001426:Bacterial Proteins; D007106:Immune Sera; D014764:Viral Proteins