Hyperchromic effect of collagen induced by human collagenase.
Loss of the highly ordered triple-helix structure of native collagen on denaturation or enzymatic degradation involves a helix-to-coil transition, which can be seen as an increase at 227 nm in its ultraviolet difference absorption spectrum. We report here the successful use of this hyperchromic effect to quantify collagen in solution and to follow up the time-course of collagen degradation catalyzed by collagenase. Using 14C-labelled collagen substrate we show the excellent correlation between enzyme-induced increase in ultraviolet difference absorption and formation of specific cleavage products. The novel method was found to be suitable to characterize the enzymatic properties of human leukocyte collagenase. Activation of latent collagenase to the active enzyme could be followed continuously and an activation lag estimated.
D000818:Animals; D002384:Catalysis; D003094:Collagen / Q000032:analysis*; D004789:Enzyme Activation; D006358:Hot Temperature; D006801:Humans; D007962:Leukocytes / Q000201:enzymology; D003012:Microbial Collagenase / Q000378:metabolism*; D011489:Protein Denaturation; D051381:Rats; D013056:Spectrophotometry, Ultraviolet
D003094:Collagen; D003012:Microbial Collagenase