Poly(dG-dC) in the Z-form inhibits E. coli DNA polymerase I and AMV DNA polymerase activity.
The effect of Z-conformation of DNA on its template activity in DNA synthesis reactions in vitro has been studied. Normal poly(dG-dC) in the B-form, brominated and unbrominated in the Z-form have been compared for their template activity in DNA synthesis reactions mediated by AMV DNA polymerase and E. coli DNA polymerase I. The results indicate that poly(dG-dC) in the Z-form is totally inactive as a template for DNA synthesis and further that it is a strong competitive inhibitor of copying of the B-form DNA.
D001354:Avian Leukosis Virus / Q000201:enzymology*; D009189:Avian Myeloblastosis Virus / Q000201:enzymology*; D002942:Circular Dichroism; D004256:DNA Polymerase I / Q000037:antagonists & inhibitors*; D004926:Escherichia coli / Q000201:enzymology*; D007700:Kinetics; D009690:Nucleic Acid Conformation; D019384:Nucleic Acid Synthesis Inhibitors; D011089:Polydeoxyribonucleotides / Q000378:metabolism*; D013698:Templates, Genetic
D019384:Nucleic Acid Synthesis Inhibitors; D011089:Polydeoxyribonucleotides; C019530:poly(dC-dG); D004256:DNA Polymerase I