Plasmid-specified aminoglycoside-modifying enzymes in clinical isolates of Klebsiella pneumoniae.
In 106 clinical isolates of multiresistant Klebsiella pneumoniae strains, we found that aminoglycoside-resistance was due mostly to two adenylating enzymes: AAD (2") (56.6%), that modifies gentamicin, kanamycin, tobramycin and sissomicin, and AAD (3") 9 (56.6% + 19.8%) that modifies streptomycin and spectinomycin. The identification of these enzymes was possible by MICs determination against a set of aminoglycosides antibiotics. AAD (2") + AAD (3")9 were coded by conjugative plasmid of about 120 Md.
D000617:Aminoglycosides / Q000378:metabolism / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000378:metabolism / Q000494:pharmacology*; D004269:DNA, Bacterial / Q000032:analysis; D004352:Drug Resistance, Microbial; D004587:Electrophoresis, Agar Gel; D006801:Humans; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D008826:Microbial Sensitivity Tests; D009713:Nucleotidyltransferases / Q000032:analysis* / Q000235:genetics; D011815:R Factors
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D009713:Nucleotidyltransferases; C020565:gentamicin 2''-nucleotidyltransferase; C022678:streptomycin 3''-adenylyltransferase