Direct formation of complexes between cytochrome P-450 and nitrosoarenes.
The mechanism of the formation of the complexes between various nitrosobenzenes and cytochrome P-450 has been investigated. We have observed the formation of these complexes by a new and, as yet, undescribed route. Nitrosobenzene (NOB) itself reacts with cytochrome P-450 in the iron(III) state, in the absence of any exogenous reducing agent, to produce the iron(II)-NOB complex. Apparently, NOB is a ligand that is capable of causing the spontaneous autoreduction of the iron. The reduction of the iron may occur via ligand-induced oxidation of the axially bound thiolate of cytochrome P-450.
D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D004578:Electron Spin Resonance Spectroscopy; D007700:Kinetics; D008862:Microsomes, Liver / Q000378:metabolism*; D009249:NADP; D009603:Nitroso Compounds / Q000378:metabolism*; D011817:Rabbits; D013053:Spectrophotometry; D013329:Structure-Activity Relationship
D009603:Nitroso Compounds; D009249:NADP; D003577:Cytochrome P-450 Enzyme System; C012456:nitrosobenzene