title: Complete nucleotide sequence of the M RNA segment of Rift Valley fever virus.
abstract: The entire M RNA segment of the phlebovirus Rift Valley fever virus (RVFV) has been molecularly cloned and the complete nucleotide sequence determined. The RNA is 3884 nucleotides in length, corresponding to a molecular weight of 1.38 X 10(6), having a base composition of 27.3% A, 25.4% G, 27.2% U, and 20.1% C. Sequences present at the 3' and 5' termini of the molecule are largely complementary for some 51 residues and can form a stable duplex structure when the potential secondary structure of the entire molecule is considered. A single major open reading frame, capable of encoding 1206 amino acids (131,845 Da), was found in the viral-complementary sequence ("positive" polarity). Amino-terminal amino acid sequencing of the purified viral glycoproteins G1 and G2 allowed for the positioning of the coding sequences for these polypeptides within this major open reading frame in the following orientation with respect to the genomic M RNA: 3'-G2-G1-5'. From the predicted amino acid composition of the two mature viral glycoproteins, both were found to have a high cysteine content (G2, 6%; G1, 5%). Sequences within the open reading frame capable of encoding up to 23,000 Da of polypeptide were found in addition to those required for the viral glycoproteins. The potential contribution of these sequences to the coding capacity of the M RNA, viral protein processing, and intracellular protein distribution is discussed.
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002043:Bunyaviridae / Q000235:genetics*; D002460:Cell Line; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D003201:Computers; D004262:DNA Restriction Enzymes; D006801:Humans; D007668:Kidney; D008168:Lung; D008253:Macaca mulatta; D009690:Nucleic Acid Conformation; D012367:RNA, Viral / Q000235:genetics*; D012296:Rift Valley fever virus / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics / Q000302:isolation & purification
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins; D004262:DNA Restriction Enzymes