Different fixative-resin combinations and the immunocytochemical demonstration of immunoglobulins (kappa light chains) in semi-thin sections.
The effect of six different epoxy resins on the immunostaining of kappa light chains in tonsilar tissue which had been fixed by one of three fixing variants is described. The results show that not only may a specific resin influence the reaction but this may be enhanced by adopting a particular fixative-resin combination.
D004853:Epoxy Resins; D005404:Fixatives; D005557:Formaldehyde; D005976:Glutaral; D006651:Histocytochemistry; D006801:Humans; D007124:Immunoenzyme Techniques; D007145:Immunoglobulin kappa-Chains / Q000032:analysis*; D008239:Lysine; D014066:Palatine Tonsil / Q000276:immunology; D010504:Periodic Acid; D010796:Phthalic Anhydrides; D011108:Polymers
D004853:Epoxy Resins; D005404:Fixatives; D007145:Immunoglobulin kappa-Chains; D010796:Phthalic Anhydrides; D011108:Polymers; C046311:periodate-lysine-paraformaldehyde; C048708:EMix; C048710:LEMix; D010504:Periodic Acid; D005557:Formaldehyde; C025599:Durcupan ACM; C005752:araldite; C048709:spurr resin; C004875:EPON; D008239:Lysine; D005976:Glutaral; C003043:paraform