title: Characterization of growth parameters of a human hepatoma cell line cultured under serum-free conditions.
abstract: The PLC/PRF/5 human hepatoma cell line has at least four complete series of hepatitis B virus (HBV)-DNA sequences integrated into the host DNA and produces hepatitis B surface antigen (HBsAg) and several serum proteins in the culture medium. In order to study serum proteins and HBsAg released by these cells under controlled conditions, the serum-free growth of several human hepatoma-derived cell lines was recently investigated. In this paper the growth of PLC/PRF/5 human hepatoma cell line in a serum- and hormone-free medium was investigated. The results represent a tool which might be used in pharmacological research, studies on hormone-binding and virus gene expression.
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000473:pathology*; D002448:Cell Adhesion; D002452:Cell Count; D002455:Cell Division; D002460:Cell Line; D003470:Culture Media; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology*
chemical_list: D003470:Culture Media