[Age-dependent characteristics of the effect of insulin on 5'-nucleotidase activity in liver plasma membranes].
Vmax of 5'-nucleotidase was found to be similar in liver plasmatic membranes of both 4-5 months old male rats and 24-26 months old animals. At the same time, Km value was higher in the membranes of old rats than in young animals. Insulin (administered at a dose of 2 un per 100 g of body mass) increased the enzyme Vmax without some age-dependent differences and decreased Km value in old animals as compared with controls.
D015720:5'-Nucleotidase; D000367:Age Factors; D000818:Animals; D002462:Cell Membrane / Q000201:enzymology; D004789:Enzyme Activation / Q000187:drug effects; D007328:Insulin / Q000494:pharmacology*; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008297:Male; D009708:Nucleotidases / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D007328:Insulin; D009708:Nucleotidases; D015720:5'-Nucleotidase
Vozrastnye osobennosti vliianiia insulina na aktivnost' 5'-nukleotidazy v plazmaticheskikh membranakh pecheni.