title: [Amplification of the adenylate cyclase gene in Escherichia coli cells].
abstract: Amplification of the cya gene of E. coli on the plasmid pBR325 leads to an increase of adenylate cyclase activity proportional to the gene dosage. In strains harboring hybrid plasmids with cya gene the intracellular level of cAMP and the rate of nucleotide secretion are also elevated. The adenylate cyclase activity in cells with truncated cya gene cloned on pBR322 remains sensitive to glucose inhibition. Amplification of the cya gene leads to considerable resistance of beta-galactosidase synthesis to transient repression by alpha-methylglucoside, but does not influence the permanent repression caused by glucose.
mesh_terms: D000262:Adenylyl Cyclases / Q000235:genetics*; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D000242:Cyclic AMP / Q000378:metabolism; D004262:DNA Restriction Enzymes; D004790:Enzyme Induction; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005784:Gene Amplification; D005796:Genes; D005798:Genes, Bacterial; D010957:Plasmids; D001616:beta-Galactosidase / Q000096:biosynthesis
chemical_list: D000242:Cyclic AMP; D004262:DNA Restriction Enzymes; D001616:beta-Galactosidase; D000262:Adenylyl Cyclases
vernacular_title: Amplifikatsiia gena adenilattsiklazy v kletkakh Escherichia coli.