Production of human active BPA from TCGF independent T cell lines that do not excrete HTLV: proof of direct action of MLA-144 derived BPA using purified BFU-E.
Burst promoting activity (BPA) and colony stimulating activity (CSA) were measured in the supernatants from six T-cell lines which are IL-2 independent and do not excrete human T-cell leukaemia virus. Two cell lines were shown to produce high levels of BPA, namely MLA-144 and the Jurkat cell line. MLA-144 is a gibbon T-cell line which can proliferate and produce BPA in serum free HB101 medium. The BPA from this line was shown to act directly on BFU-E by studies using highly purified peripheral blood derived BFU-E. The Jurkat CM had far less activity against purified progenitors. These cells lines will be of value in the study of erythropoietic regulation.
D000818:Animals; D001853:Bone Marrow / Q000473:pathology; D002455:Cell Division; D002460:Cell Line; D003115:Colony-Stimulating Factors / Q000032:analysis / Q000096:biosynthesis*; D003470:Culture Media; D017977:Deltaretrovirus; D004912:Erythrocytes / Q000473:pathology; D006412:Hematopoietic Stem Cells / Q000473:pathology; D006801:Humans; D007376:Interleukin-2 / Q000502:physiology*; D007942:Leukemia, Experimental / Q000378:metabolism*; D013601:T-Lymphocytes
D003115:Colony-Stimulating Factors; D003470:Culture Media; D007376:Interleukin-2