Alteration in cyclic adenosine 3':5'-monophosphate binding proteins during the phenotypic change of mouse macrophages transformed by a temperature-sensitive mutant (tsA640) of SV40.
By using a photoaffinity ligand, cell extracts from transformed macrophages that were established by infection with temperature-sensitive mutants (tsA640) of simian virus 40 (SV40) were examined for cyclic adenosine 3':5'-monophosphate (cAMP)-binding proteins. At the nonpermissive temperature for SV40 large T antigen, 39.0 degrees C, no significant cAMP-binding proteins could be detected, such as primary mouse macrophages. At the permissive temperature of 33.0 degrees C, cAMP-binding proteins appeared later than SV40 T antigen expression and cellular DNA synthesis. The profile of cAMP-binding proteins was similar to that of resting, but not proliferating, mouse clonal fibroblasts (BALB/c 3T3). These and previous results suggest that SV40 T antigen influences the expression of cAMP-binding proteins in tsA640-transformed macrophages; the large/small T antigen converts the profile of cAMP-binding proteins from macrophage to fibroblastic cells.
D000818:Animals; D000957:Antigens, Viral, Tumor; D002352:Carrier Proteins / Q000378:metabolism*; D002460:Cell Line; D002472:Cell Transformation, Viral; D000242:Cyclic AMP / Q000378:metabolism; D002373:Cyclic AMP Receptor Protein; D004247:DNA / Q000096:biosynthesis; D007399:Interphase; D007700:Kinetics; D008264:Macrophages / Q000378:metabolism* / Q000382:microbiology / Q000502:physiology; D051379:Mice; D009154:Mutation; D010641:Phenotype; D013539:Simian virus 40 / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D013696:Temperature
D000957:Antigens, Viral, Tumor; D002352:Carrier Proteins; D002373:Cyclic AMP Receptor Protein; D004247:DNA; D000242:Cyclic AMP