Gene activation properties of a mouse DNA sequence isolated by expression selection.
The MES-1 element was previously isolated from restricted total mouse cellular DNA by "expression selection"--the ability to reactivate expression of a test gene devoid of its 5' enhancer sequences. Mes-1 has been tested in long-term transformation and short-term CAT expression assays. In both assays MES-1 is active independent of orientation and at a distance when placed 5' to the test gene. The element is active with heterologous promoters and functions efficiently in both rat and mouse cells. MES-1 activates expression by increasing transcription from the test gene's own start (cap) site. Thus the expression selection technique can be used for the isolation of DNA sequences with enhancer-like properties from total cellular DNA.
D000123:Acetyltransferases / Q000235:genetics; D000667:Ampicillin / Q000494:pharmacology; D000818:Animals; D001483:Base Sequence; D015500:Chloramphenicol O-Acetyltransferase; D004262:DNA Restriction Enzymes; D004742:Enhancer Elements, Genetic; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics; D005809:Genes, Regulator; D005822:Genetic Vectors; D051379:Mice; D010403:Penicillin Resistance; D010957:Plasmids; D014158:Transcription, Genetic
D000667:Ampicillin; D000123:Acetyltransferases; D015500:Chloramphenicol O-Acetyltransferase; D004262:DNA Restriction Enzymes