[Use of a microvirus-neutralizing reaction in diagnosing transmissible gastroenteritis (TGE)].
A micro virus-neutralization reaction was designed and tested to detect antibodies to the virus of transmissive gastroenteritis. Use was made of a stable cell line, SPEV, and a laboratory strain of the virus that had been adapted to it. The optimal concentration values of the cell suspension and the normal calf serum contained in it were determined. A total of 90 blood serum samples from pigs were comparatively investigated for the presence of virus-neutralising TGE antibodies, the tube test being performed trough the inoculation of the cells in suspension. On the other hand, the micro virus-neutralization test was carried out in two variants: the sera were diluted via Mikrotiter micropipettes and micropipettes of the Takachi apparatus. It was found that the micro virus-neutralization test in its two variants was not inferior in terms of sensitivity to the tube test, was more readily applicable, and was less material consuming.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D003332:Coronaviridae / Q000276:immunology*; D005761:Gastroenteritis, Transmissible, of Swine / Q000175:diagnosis*; D009500:Neutralization Tests / Q000379:methods / Q000662:veterinary; D013552:Swine; D005760:Transmissible gastroenteritis virus / Q000276:immunology*; D014776:Virus Cultivation / Q000379:methods
D000914:Antibodies, Viral
Izpolzuvane na mikro-virusneutralizatsionnata reaktsiia v diagnostikata na transmisivniia gastroenterit (TGE).