title: Proteins expressed by Mill Door/79 virus, a Kemerovo serogroup orbivirus transmitted by the ticks Ixodes uriae.
abstract: Mill Door/79 virus, an orbivirus of the Kemerovo serogroup, Great Island Complex, was shown to induce in infected cells 10 segments of double-stranded RNA with a total molecular weight of 11.46 X 10(6) daltons. Using methyl mercuric hydroxide as a denaturing agent the double-stranded RNA was translated in a cell-free system producing 11 polypeptides, 10 of which co-migrated with those produced in Mill Door/79 virus infected Vero cells. The segments were separated and individually translated in a cell-free system allowing their coding assignments to be made. These assignments were confirmed by partial proteolysis.
mesh_terms: D000818:Animals; D002460:Cell Line; D002474:Cell-Free System; D002522:Chlorocebus aethiops; D007668:Kidney; D018163:Orbivirus / Q000302:isolation & purification / Q000378:metabolism*; D014176:Protein Biosynthesis; D012330:RNA, Double-Stranded / Q000302:isolation & purification / Q000378:metabolism; D012367:RNA, Viral / Q000302:isolation & purification / Q000378:metabolism; D012087:Reoviridae / Q000378:metabolism*; D013987:Ticks / Q000382:microbiology*; D014764:Viral Proteins / Q000096:biosynthesis*
chemical_list: D012330:RNA, Double-Stranded; D012367:RNA, Viral; D014764:Viral Proteins