title: Varicella-zoster virus p32/p36 complex is present in both the viral capsid and the nuclear matrix of the infected cell.
abstract: Varicella-zoster virus (VZV) directs the synthesis of numerous glycosylated and nonglycosylated infected-cell-specific proteins, many of which are later incorporated into the virion as structural components. In this study, we characterized a nonglycosylated polypeptide complex with the aid of a VZV-specific murine monoclonal antibody clone, 251D9. As detected by indirect immunofluorescence, the antibody bound mainly to antigens located within the nuclei of infected cells and did not attach to an uninfected cell substrate. The polypeptide specificity of the monoclonal antibody was determined by immunoblot analysis of electrophoretically separated infected cell extracts to react with a 32,000-molecular-weight VZV-specific protein (p32); in addition, the antibody also bound to a 36,000-molecular-weight polypeptide. The synthesis of these antigens was unaffected by inhibitors of glycosylation. Nonionic or ionic detergents were only marginally effective in solubilization of the p32-p36 complex, and relatively small amounts were eluted from nuclei by high salt concentrations (2 M NaCl). The same proteins remained associated with the nuclear matrix of VZV-infected cells. We also demonstrated that the protein complex was a major component of purified VZV nucleocapsids; p32 was especially prominent in both full and empty capsids. Immunoblot analysis of the nucleocapsid preparation revealed two additional species (p34 and p38) in the p32-p36 complex. Phosphorylation was a distinctive feature of some of the constituents. In summary, these results indicate that the p32-p36 complex represents a family of structural proteins closely associated with the assembly of VZV nucleocapsids and the encapsidation of viral DNA.
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology; D002213:Capsid / Q000032:analysis; D002467:Cell Nucleus / Q000032:analysis*; D002478:Cells, Cultured; D003429:Cross Reactions; D004261:DNA Replication; D004268:DNA-Binding Proteins / Q000302:isolation & purification; D006564:Herpesviridae / Q000276:immunology; D014645:Herpesvirus 3, Human / Q000032:analysis* / Q000502:physiology; D006801:Humans; D008545:Melanoma; D008970:Molecular Weight; D010750:Phosphoproteins / Q000276:immunology / Q000302:isolation & purification; D012995:Solubility; D014764:Viral Proteins / Q000276:immunology / Q000302:isolation & purification*; D014779:Virus Replication
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D004268:DNA-Binding Proteins; D010750:Phosphoproteins; D014764:Viral Proteins