title: Comparison of the primate alphaherpesviruses. I. Characterization of two herpesviruses from spider monkeys and squirrel monkeys and viral polypeptides synthesized in infected cells.
abstract: Biological and biochemical properties of two neurotropic herpesviruses of New World monkeys--Herpesvirus saimiri type 1 (HVS-1) and Herpesvirus ateles type 1 (HVA-1)--were examined and compared. HVS-1 and HVA-1 both exhibited a time course of replication similar to another primate herpesvirus, SA 8. Both viruses grew rapidly and high titers of infectious virus were readily produced. HVS-1 and HVA-1 were also able to replicate efficiently in cell lines derived from a number of primate and non-primate species. Analysis of proteins synthesized in infected cells revealed the presence of over 30 virus-specific proteins ranging from less than 30,000 to over 200,000 daltons apparent molecular weight. Both viruses specified synthesis of a major capsid polypeptide of 148,000 daltons. Pulse labeling of cells during infection demonstrated temporal differences in the kinetics of synthesis of individual viral proteins and post-translational modification of a number of viral polypeptides. Glycosylated polypeptides synthesized in HVS-1 and HVA-1 infected cells were identified which ranged from approximately 49,000 to 120,000 daltons. Structural polypeptides of HVA-1 and HVS-1 virions were identified by SDS-PAGE analysis of purified virions. Taken together with clinical data on the diseases caused by these viruses, these studies indicate that HVS-1 and HVA-1 appear similar in many respects to both the human herpes simplex viruses and alphaherpesviruses of other primates.
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000096:biosynthesis; D002427:Cebidae / Q000382:microbiology; D002478:Cells, Cultured; D006023:Glycoproteins / Q000096:biosynthesis / Q000276:immunology; D006564:Herpesviridae / Q000145:classification*; D006567:Herpesvirus 2, Saimiriine / Q000145:classification*; D008970:Molecular Weight; D011499:Protein Processing, Post-Translational; D012453:Saimiri / Q000382:microbiology; D013045:Species Specificity; D013997:Time Factors; D014764:Viral Proteins / Q000096:biosynthesis / Q000276:immunology; D014779:Virus Replication
chemical_list: D000956:Antigens, Viral; D006023:Glycoproteins; D014764:Viral Proteins