The purification of alphavirus virions and subviral particles using ultrafiltration and gel exclusion chromatography.
Conventional methods of virus purification using ultracentrifugation frequently result in distorted particles with low levels of biological activity, and are thus unsuitable for preparing samples for high-resolution techniques such as neutron scattering, X-ray scattering in solution, and X-ray crystallography. Moreover, in the event of an instrument failure, ultracentrifugation can also pose a significant hazard when preparing pathogenic viruses or subunits derived from them. By employing exclusively ultrafiltration and gel exclusion chromatography, a method has been developed to prepare highly purified, intact alphavirus particles retaining high levels of biological activity. These procedures have also been adapted to prepare aggregates of viral envelope protein with a defined immunogenic content.
D000524:Alphavirus / Q000276:immunology / Q000302:isolation & purification* / Q000648:ultrastructure; D000956:Antigens, Viral / Q000302:isolation & purification; D002850:Chromatography, Gel / Q000379:methods*; D008854:Microscopy, Electron; D014462:Ultrafiltration / Q000379:methods*; D014764:Viral Proteins / Q000302:isolation & purification
D000956:Antigens, Viral; D014764:Viral Proteins