title: Nucleotide sequence of the Saccharomyces cerevisiae MET25 gene.
abstract: To elucidate further the molecular basis of the specific regulatory mechanism modulating the expression of the genes implicated in methionine metabolism, we have cloned and characterized two genes, MET3 and MET25, and shown that the regulation of their expression is transcriptional. The sequence of the cloned yeast MET25 gene which encodes the O-acetyl homoserine - O-acetyl serine (OAH-OAS) sulfhydrylase is reported here along with its 5' and 3' flanking regions. The amino acid composition predicted from the DNA sequence is in good agreement with that determined by hydrolysis of the purified enzyme. In the 5' flanking region the signal for general amino acid control was not found, corroborating our previous finding that the synthesis of OAH-OAS sulfhydrylase is not submitted to general control. The transcription start points have been determined. The 5' and 3' flanking regions of the MET25 gene suggest initiation and termination signals similar to those associated with other yeast genes.
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D019757:Carbon-Oxygen Lyases; D003062:Codon; D003547:Cysteine Synthase; D005786:Gene Expression Regulation; D005800:Genes, Fungal; D008190:Lyases / Q000235:genetics*; D008715:Methionine / Q000235:genetics*; D009097:Multienzyme Complexes; D009713:Nucleotidyltransferases / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D013430:Sulfate Adenylyltransferase / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D003062:Codon; D009097:Multienzyme Complexes; D029701:Saccharomyces cerevisiae Proteins; D008715:Methionine; D003547:Cysteine Synthase; C103381:MET17 protein, S cerevisiae; C018725:O-acetylhomoserine (thiol)-lyase; D009713:Nucleotidyltransferases; D013430:Sulfate Adenylyltransferase; D008190:Lyases; D019757:Carbon-Oxygen Lyases