[The role of ATP and membrane potential in the penetration of phage T17 DNA into the cell during infection].
The role of ATP and membrane potential in phage T7 DNA injection into E. coli during infection has been studied. Entrance of phage T7 genes of class II and III was shown to be prevented by arsenate, indicating the requirement for phosphorylated macroergs in the phage DNA injection. The injection process was also inhibited by exposition of the cells to the uncoupler of oxidative phosphorylation. Dependence of the injection efficiency on the membrane-potential value has been shown to be sigmoidal, which suggests a regulatory role of the membrane potential in phage T7 DNA injection from the virion into the host cell.
D000255:Adenosine Triphosphate / Q000378:metabolism*; D001149:Arsenates / Q000494:pharmacology; D002463:Cell Membrane Permeability; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000235:genetics* / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D005814:Genes, Viral; D007700:Kinetics; D008564:Membrane Potentials; D013604:T-Phages / Q000235:genetics*; D014162:Transfection; D014771:Virion / Q000235:genetics
D001149:Arsenates; D004279:DNA, Viral; D000255:Adenosine Triphosphate; D004262:DNA Restriction Enzymes
Rol' ATP i membrannogo potentsiala v protsesse proniknoveniia DNK faga T17 v kletku pri infektsii.