[Perfusion of monolayer-cultured B cells of the neonatal rat pancreas--effect of 2-deoxy-2-fluoroglucose].
The effects of 2-deoxy-2-fluoroglucose in promoting the function of neonatal rat pancreatic B cells in vitro are presented. Functional studies of insulin secretion were carried out by perifusion procedures. At day 0 of culture, B cells showed a minimal monophasic insulin secretion in response to a 16.7 mM single dose of glucose, whereas in the presence of 10 microM forskolin or 1 mM 3-isobutyl-1-methylxanthine, the same dose of glucose stimulated insulin secretion in a biphasic fashion. In contrast, after 7 days of culture in medium supplemented with 1 mM 2-deoxy-2-fluoroglucose, B cells showed an adult-like biphasic pattern regardless of the presence of forskolin or 3-isobutyl-1-methylxanthine. In addition, the stimulatory effect of either leucine or 2-ketoisocaproate was also significantly increased, when compared to that of B cells at day 0. Furthermore, when exposed to a linear gradient stimulation by glucose, these competent B cells secreted insulin in a dose-dependent fashion. Moreover, in cultures supplemented with 2-deoxy-2-fluoroglucose the basal or stimulating levels of cAMP were about 20-fold higher than at day 0. In conclusion, the data presented here demonstrate that the function of neonatal B cells matures during culture, and suggest that this effect of 2-deoxy-2-fluoroglucose may be mediated by activation of either adenylate cyclase or catabolic enzymes of amino acids in B cells.
D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D003837:Deoxy Sugars / Q000494:pharmacology*; D003847:Deoxyglucose / Q000031:analogs & derivatives / Q000494:pharmacology*; D019788:Fluorodeoxyglucose F18; D005947:Glucose / Q000494:pharmacology; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000187:drug effects / Q000378:metabolism*; D007651:Keto Acids / Q000494:pharmacology; D007930:Leucine / Q000494:pharmacology; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains; D013268:Stimulation, Chemical
D003837:Deoxy Sugars; D007328:Insulin; D007651:Keto Acids; D019788:Fluorodeoxyglucose F18; C013082:alpha-ketoisocaproic acid; D004247:DNA; D003847:Deoxyglucose; D000242:Cyclic AMP; D007930:Leucine; D005947:Glucose