Periportal and perivenous hepatocytes retain their zonal characteristics in primary culture.
Periportal and perivenous hepatocytes from rat liver were isolated by combined digitonin-collagenase perfusion, and gluconeogenesis, urea synthesis and fatty acid synthesis was measured both in freshly isolated cells and in primary culture. A periportal zonation of gluconeogenesis and urea synthesis of about 3 and 1.5 fold, respectively, was observed. This zonation persisted unchanged for 23 hours in culture under identical conditions of incubation for periportal and perivenous cells. Fatty acid synthesis was not zonated.
D000410:Alanine Transaminase / Q000378:metabolism; D000818:Animals; D002478:Cells, Cultured; D004072:Digitonin / Q000494:pharmacology; D005227:Fatty Acids / Q000096:biosynthesis; D005260:Female; D005943:Gluconeogenesis; D008099:Liver / Q000166:cytology*; D003012:Microbial Collagenase / Q000378:metabolism; D011770:Pyruvate Kinase / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors; D014508:Urea / Q000096:biosynthesis
D005227:Fatty Acids; D014508:Urea; D000410:Alanine Transaminase; D011770:Pyruvate Kinase; D003012:Microbial Collagenase; D004072:Digitonin