Actin filaments and tumorigenicity in a Fischer rat embryo fibroblast cell line (3Y1) transformed by ultraviolet-irradiated HSV.
The characteristics of the cytoskeleton of a Fischer rat embryo fibroblast cell line (3Y1) transformed by ultraviolet (UV)-irradiated HSV were studied by indirect immunofluorescence using anti-actin IgG. Parental 3Y1 cells possessed well-developed actin filaments, while 3Y1 cells transformed by UV-irradiated HSV also retained well-developed actin filaments. Transformed cells were divided into 2 groups according to tumorigenicity in newborn Fischer rats; one had a strongly tumorigenic potential and the other a weakly tumorigenic potential. Tumor-derived cell lines exhibited a highly tumorigenic potential, and were also divided into 2 groups, one with well-developed actin filaments and the other without well-developed actin filaments. Our results suggested that transformation or tumor formation by HSV is a multi-step process and that morphological loss of actin filaments in the cells is not essential to the tumorigenic potential of the cells transformed by HSV.
D000199:Actins / Q000032:analysis*; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000648:ultrastructure*; D002472:Cell Transformation, Viral; D003599:Cytoskeleton / Q000648:ultrastructure*; D004279:DNA, Viral / Q000032:analysis; D005347:Fibroblasts / Q000648:ultrastructure; D051381:Rats; D011916:Rats, Inbred F344; D018139:Simplexvirus / Q000472:pathogenicity* / Q000528:radiation effects
D000199:Actins; D004279:DNA, Viral