DNA alterations photosensitized by tetracycline and some of its derivatives.
Bacteriophage M13 mp10 DNA were irradiated with near-UV light in the presence of tetracycline derivatives and primed with synthetic oligonucleotide to be used for DNA synthesis using Escherichia coli DNA polymerase. Chain terminations were observed by denaturing polyacrylamide gel electrophoresis and mapped precisely. All the synthesis stops occurred before or at the level of guanine residues, showing that the photoreaction mediated by tetracycline derivatives led to a preferential alteration of guanine residues. These lesions were demonstrated to be induced in DNA through a pathway involving singlet oxygen. Tetracycline derivatives also photoinduced the breakage of the DNA sugar-phosphate backbone monitored by the conversion of supercoiled phi X174 DNA to a relaxed form. This lesion was shown to be initiated by hydroxyl radicals. The production of this free radical has been confirmed by electron paramagnetic resonance (EPR) spin trapping experiments using 5,5-dimethyl-1-pyrroline-N-oxide as spin trap. In addition to the EPR signal due to OH radicals trapping another unassigned signal has been detected.
D000818:Animals; D004247:DNA / Q000528:radiation effects*; D004277:DNA, Single-Stranded / Q000528:radiation effects; D004578:Electron Spin Resonance Spectroscopy; D010787:Photosensitivity Disorders; D013752:Tetracycline / Q000494:pharmacology*
D004277:DNA, Single-Stranded; D004247:DNA; D013752:Tetracycline