Enzyme-linked immunosorbent assay for the detection of antibodies to bovine viral diarrhea virus in bovine sera.
A specific and sensitive enzyme-linked immunosorbent assay (ELISA) was established for the detection of antibodies to bovine viral diarrhea virus (BVDV) in bovine sera. Polyethylene-glycol concentrated, equilibrium density gradient purified BVDV was used as test antigen at an optimal amount of 1 microgram/well, whereas the optimal concentration of conjugate was at 1/2000 dilution. The standardized test encountered no non-specific reaction with test sera at a starting dilution of 1/10. A total of 50 bovine serum samples was assayed for the presence of antibodies against BVDV by ELISA and serum neutralization test (SNT). A positive correlation between the 2 tests was found. However, ELISA could be as much as 500-fold more sensitive than SNT in detecting low levels of BVDV antibodies.
D000034:Abortion, Veterinary / Q000276:immunology; D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000175:diagnosis / Q000276:immunology; D002417:Cattle / Q000276:immunology*; D001908:Diarrhea Viruses, Bovine Viral / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007124:Immunoenzyme Techniques; D009500:Neutralization Tests; D010576:Pestivirus / Q000276:immunology*; D011247:Pregnancy
D000914:Antibodies, Viral