Bradykinin-induced changes in phosphatidyl inositol turnover in cultured rabbit papillary collecting tubule cells.
Rabbit renal papillary collecting tubule cells were isolated as a homogeneous population and grown in primary culture. These cells were maintained in fully defined medium to inhibit fibroblast overgrowth and to facilitate labeling of endogenous inositol phospholipids with myo-[2-3H]inositol with high specific activity. These cells demonstrated the morphology, cyclic AMP responsiveness, and prostaglandin E2 (PGE2) elaboration, consistent with previous published characterizations. When cells labeled with myo-[2-3H]inositol were stimulated by bradykinin at 10(-7) M, time-dependent and reversible changes in the distribution of inositol polyphosphates were observed. Inositol 1,4,5-triphosphate and inositol 1,4-diphosphate showed time-dependent and dose-dependent increases to maximal levels of 225 and 223% of control, respectively. These data indicate that the elaboration of inositol polyphosphates is a biochemical correlate to bradykinin stimulation and may play a role in PGE2 release in renal papillary collecting tubule cells.
D000818:Animals; D001920:Bradykinin / Q000494:pharmacology*; D002455:Cell Division; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000096:biosynthesis; D015232:Dinoprostone; D005260:Female; D007295:Inositol Phosphates / Q000378:metabolism; D007679:Kidney Medulla / Q000166:cytology / Q000378:metabolism*; D007684:Kidney Tubules / Q000378:metabolism*; D007685:Kidney Tubules, Collecting / Q000378:metabolism*; D010716:Phosphatidylinositols / Q000032:analysis / Q000378:metabolism*; D011458:Prostaglandins E / Q000096:biosynthesis; D011817:Rabbits
D007295:Inositol Phosphates; D010716:Phosphatidylinositols; D011458:Prostaglandins E; D000242:Cyclic AMP; D015232:Dinoprostone; D001920:Bradykinin