Cloning and characterization of the Schizosaccharomyces pombe DNA ligase gene CDC17.
The Schizosaccharomyces pombe CDC17 gene has been cloned by complementation of the cdc17 mutant coding for temperature-sensitive DNA ligase. An allele-specific suppressor active only in the presence of a high osmotic pressure was also isolated. The cloned CDC17 gene failed to complement the analogous DNA ligase mutation, cdc9, in Saccharomyces cerevisiae, although the reverse complementation was successful [Barker and Johnston, Eur. J. Biochem. 134 (1983) 315-319]. The CDC17 gene specifies a 2.8-kb transcript.
D003001:Cloning, Molecular; D011088:DNA Ligases / Q000235:genetics* / Q000378:metabolism; D004262:DNA Restriction Enzymes; D005796:Genes; D005800:Genes, Fungal; D005816:Genetic Complementation Test; D009154:Mutation; D011118:Polynucleotide Ligases / Q000235:genetics*; D004718:Saccharomycetales / Q000235:genetics*; D012568:Schizosaccharomyces / Q000201:enzymology / Q000235:genetics*; D013696:Temperature; D014158:Transcription, Genetic
D004262:DNA Restriction Enzymes; D011088:DNA Ligases; D011118:Polynucleotide Ligases