Cholinergic muscarinic receptor in the nasal mucosa of guinea pigs with bronchial asthma with and without hypersensitive nasal symptoms.
Using [3H]-quinuclidinyl benzilate (QNB) as a radioligand, receptor binding assay was performed to evaluate the changes of the muscarinic receptor in the nasal mucosa of guinea pigs sensitized with toluene diisocyanate (TDI), which showed hypersensitive airway symptoms both in the lower airway and in the nose. The same study was performed in guinea pigs sensitized with bacterial crystalline alpha-amylase (BCA) which showed typical symptoms of bronchial asthma but without hypersensitive nasal symptoms. In the nasal mucosa of guinea pigs sensitized with TDI, an increased density of the muscarinic receptor was confirmed with no change in affinity of the receptor. However, in the nasal mucosa of guinea pigs sensitized with BCA, no changes were observed either in the density of the muscarinic receptor of the nasal mucosa or in its affinity. The increased density of the muscarinic receptor observed in subjects with nasal allergy, which was reported earlier, is not specifically related to IgE-mediated nasal allergy and is not induced by systemic sensitization itself but is assumed to be induced by some pathophysiological changes in the nasal mucosa including antigen-antibody reaction with development of hypersensitive nasal symptoms.
D000818:Animals; D001249:Asthma / Q000276:immunology*; D001665:Binding Sites; D006168:Guinea Pigs; D007073:Immunoglobulin E / Q000276:immunology; D008297:Male; D009297:Nasal Mucosa / Q000276:immunology*; D011950:Receptors, Cholinergic / Q000276:immunology*; D011976:Receptors, Muscarinic / Q000276:immunology*; D012220:Rhinitis / Q000276:immunology*; D014051:Toluene 2,4-Diisocyanate; D000516:alpha-Amylases
D011950:Receptors, Cholinergic; D011976:Receptors, Muscarinic; D014051:Toluene 2,4-Diisocyanate; D007073:Immunoglobulin E; D000516:alpha-Amylases