[The role of adenylate kinase in the regulation of the rate and effectiveness of energy transfer from mitochondria to hexokinase in vitro].
The effect of adenylate kinase activity on the rate and efficiency of energy transport from mitochondria to hexokinase was studied in a system containing isolated rabbit heart mitochondria, hexokinase and adenylate kinase at low concentrations of adenine nucleotides. Oxygen consumption by mitochondria and glucose-6-phosphate synthesis by hexokinase were recorded. It was found that with adenylate kinase being active both in mitochondria and in the washing solution, the rate and efficiency of glucose-6-phosphate synthesis considerably increases. The effects of adenylate kinase activity are fully abolished by diadenosine pentaphosphate, an inhibitor of adenylate kinase. The experimental results based on the use of adenylate kinase demonstrate the possibility of increasing the rate and efficiency of energy transfer between two spatially uncoupled biochemical processes in vitro with the aid of an enzymatic system.
D000263:Adenylate Kinase / Q000378:metabolism*; D000818:Animals; D003593:Cytoplasm / Q000201:enzymology; D004734:Energy Metabolism; D019298:Glucose-6-Phosphate; D005958:Glucosephosphates / Q000096:biosynthesis; D006593:Hexokinase / Q000378:metabolism*; D066298:In Vitro Techniques; D007700:Kinetics; D008929:Mitochondria, Heart / Q000201:enzymology / Q000378:metabolism*; D008954:Models, Biological; D010770:Phosphotransferases / Q000378:metabolism*; D011817:Rabbits
D005958:Glucosephosphates; D019298:Glucose-6-Phosphate; D010770:Phosphotransferases; D006593:Hexokinase; D000263:Adenylate Kinase
Issledovanie roli adenilatkinazy v reguliatsii skorosti i éffektivnosti peredachi énergii ot mitokhondriĭ k geksokinaze in vitro.