Heme regulates the expression in Saccharomyces cerevisiae of chimaeric genes containing 5'-flanking soybean leghemoglobin sequences.
The TM1 yeast mutant was transformed with a 2 micron-derived plasmid (YEp24) which carries a chimaeric gene containing the Escherichia coli chloramphenicol acetyl transferase (CAT) gene fused to the 5'- and 3'-flanking regions of the soybean leghemoglobin (Lb) c3 gene. Expression of the chimaeric CAT gene is controlled specifically by heme at a post-transcriptional level, most likely by regulating the efficiencies of translation. Expression of another chimaeric gene consisting of the neomycin phosphotransferase (NPTII) gene fused to only the 5'-flanking region of the Lbc3 gene is regulated by heme in a similar way. Thus, in yeast, heme modulates the translation of the chimaeric mRNAs through interactions with the 5' Lbc3 non-coding region.
D000123:Acetyltransferases / Q000235:genetics; D001483:Base Sequence; D002678:Chimera; D015500:Chloramphenicol O-Acetyltransferase; D004262:DNA Restriction Enzymes; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D005796:Genes / Q000187:drug effects; D005798:Genes, Bacterial; D005822:Genetic Vectors; D006418:Heme / Q000494:pharmacology*; D006420:Hemeproteins / Q000235:genetics*; D007874:Leghemoglobin / Q000235:genetics*; D009154:Mutation; D010944:Plants / Q000235:genetics*; D010957:Plasmids; D012323:RNA Processing, Post-Transcriptional; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics*; D013025:Glycine max; D014158:Transcription, Genetic
D006420:Hemeproteins; D007874:Leghemoglobin; D006418:Heme; D000123:Acetyltransferases; D015500:Chloramphenicol O-Acetyltransferase; D004262:DNA Restriction Enzymes