title: Beta-glucuronidase mutants of the nematode Caenorhabditis elegans.
abstract: Using a screening procedure that is based on a histochemical stain for the enzyme beta-glucuronidase, we have isolated several mutants of the nematode Caenorhabditis elegans affected in beta-glucuronidase activity. All of the mutations fall into one complementation group and identify a new gene, gus-1, which has been mapped on the right arm of linkage group I (LG I), 1.1 map units to the left of unc-54. The mutants have no visible phenotype, and their viabilities and fertilities are unaffected. Linked revertants of two of the mutations have been isolated. They restore enzyme activity to almost wild-type levels; the beta-glucuronidase that one of the revertants produces differs from that of the wild type. We propose that gus-1 is the structural locus for beta-glucuronidase.
mesh_terms: D000483:Alleles; D000818:Animals; D002107:Caenorhabditis / Q000201:enzymology / Q000235:genetics*; D004262:DNA Restriction Enzymes; D005796:Genes; D005966:Glucuronidase / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D007700:Kinetics; D009154:Mutation; D013045:Species Specificity; D013816:Thermodynamics
chemical_list: D004262:DNA Restriction Enzymes; D005966:Glucuronidase