Rat carcinoma cells in long-term, serum-free culture provide a continuing supply of collagenase.
Neoplastic, epithelial cells derived from a spontaneously-arising rat mammary carcinoma have been cultured in a defined medium, in the absence of serum, continuously, for over 2 years. The medium is a mixture of Ham's F12 and Dulbecco's Modified Eagle's media supplemented with insulin, transferrin and bovine serum albumin. The cells have retained their potential to produce tumours and, in culture, a true vertebrate collagenase. This system provides a continuing supply of vertebrate collagenase through the application of recently developed methods.
D000818:Animals; D001790:Blood Physiological Phenomena; D002277:Carcinoma / Q000201:enzymology* / Q000473:pathology; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000378:metabolism / Q000473:pathology; D003470:Culture Media / Q000032:analysis* / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005470:Fluorometry; D006133:Growth Substances / Q000494:pharmacology; D008325:Mammary Neoplasms, Experimental / Q000201:enzymology* / Q000473:pathology; D003012:Microbial Collagenase / Q000302:isolation & purification / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
D003470:Culture Media; D006133:Growth Substances; D003012:Microbial Collagenase