Role of VSV G antigen in the development of experimental spongiform encephalopathy in mice.
The ts G31 VSV mutant induced spongiform encephalopathy without any inflammatory response when injected i.c. into the mouse. In electron microscopy, no virions could be detected in spongiform lesions. In contrast, with the wild VSV strain inflammatory lesions were seen, which contained viral particles in great abundance. As previously shown in vitro, when using the ts G 31 mutant at the nonpermissive temperature, the G antigen can spread from membrane to membrane to distant sites, fusing a great number of cells even in the absence of virus multiplication. Therefore, we postulate that a comparable mechanism is responsible for extensive brain lesions originating probably from a relatively small number of G antigen-producing cells. Indeed, the spongious regions seen mainly in the grey matter contained vacuoles, whose walls were clearly stained by peroxidase-labelled immune serum to G antigen, without detectable virions or inflammatory lesions. The vacuoles probably represent altered and swollen dendritic cell membranes. The relationship between spongiosis development and antigen diffusion in the absence of significant virus replication is discussed.
D000818:Animals; D000956:Antigens, Viral / Q000378:metabolism*; D001927:Brain Diseases / Q000378:metabolism / Q000382:microbiology* / Q000473:pathology; D002540:Cerebral Cortex / Q000382:microbiology / Q000473:pathology; D005260:Female; D007124:Immunoenzyme Techniques; D008562:Membrane Glycoproteins; D051379:Mice; D009474:Neurons / Q000378:metabolism / Q000382:microbiology / Q000473:pathology; D013116:Spinal Cord / Q000473:pathology; D014721:Vesicular stomatitis Indiana virus / Q000276:immunology / Q000378:metabolism; D014759:Viral Envelope Proteins; D014764:Viral Proteins / Q000378:metabolism*; D014777:Virus Diseases / Q000378:metabolism / Q000473:pathology*
D000956:Antigens, Viral; C028391:G protein, vesicular stomatitis virus; D008562:Membrane Glycoproteins; D014759:Viral Envelope Proteins; D014764:Viral Proteins