Photosensitization of mitochondrial adenosine-triphosphatase and adenylate kinase by hematoporphyrin derivative in vitro.
Mitochondrial ATPase and adenylate kinase activity of hepatoma cells were inhibited by hematoporphyrin derivative (HPD) followed by photoirradiation. Inhibition of ATPase activity was a dose- and time-related event. Malonaldehyde (MDA) content of mitochondrial membranes was markedly increased by HPD plus light. The content of mouse liver microsomal cytochrome P-450 was greatly increased after intraperitoneal injection of HPD for 4 days (5 mg/kg/day). The liver weight, and levels of liver microsomal G-6-phosphatase, MDA and triglyceride (TG) showed no difference in treated vs. control animals. The data presented here demonstrate that mitochondria may be a sensitive site of action of HPD photosensitization, and inactivation of ATPase and adenylate kinase may be an important contributing factor to tumor cell damage and death.
D000251:Adenosine Triphosphatases / Q000378:metabolism* / Q000528:radiation effects; D000263:Adenylate Kinase / Q000378:metabolism* / Q000528:radiation effects; D000818:Animals; D005260:Female; D005952:Glucose-6-Phosphatase / Q000378:metabolism / Q000528:radiation effects; D017324:Hematoporphyrin Derivative; D006414:Hematoporphyrin Photoradiation; D006415:Hematoporphyrins / Q000494:pharmacology*; D007700:Kinetics; D008114:Liver Neoplasms, Experimental / Q000188:drug therapy / Q000201:enzymology*; D051379:Mice; D010770:Phosphotransferases / Q000378:metabolism*; D011838:Radiation-Sensitizing Agents / Q000494:pharmacology*
D006415:Hematoporphyrins; D011838:Radiation-Sensitizing Agents; D017324:Hematoporphyrin Derivative; D010770:Phosphotransferases; D000263:Adenylate Kinase; D005952:Glucose-6-Phosphatase; D000251:Adenosine Triphosphatases