The effect of dietary bagasse on the activities of some key enzymes of carbohydrate and lipid metabolism in mouse liver.
The effects of a 100 g/kg diet substitution of bagasse on the body-weight gain, food consumption and faecal dry weight of mice given a high-sucrose diet and on the activities of hepatic glucose-6-phosphate dehydrogenase (EC I.I.I.49), 6-phosphogluconate dehydrogenase (EC I.I.I.44), malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) (EC I.I.I.40), ATP-citrate (pro-3S) lyase (EC 4.I.3.8), 6-phosphofructokinase EC 2.7.I.II), pyruvate kinase (EC 2.7.I.40) and fructose-1,6-bisphosphatase (EC 3.I.3.II) were studied. Bagasse had no effect on body-weight gain, food consumption or faecal dry weight. Bagasse decreased the activities of glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase and phosphofructokinase expressed on a wet weight basis and on a protein basis. Bagasse decreased the activities of glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase expressed on a body-weight basis. These results suggest that bagasse decreases the flux through some pathways of hepatic lipogenesis when mice are given high-sucrose diets.
D000818:Animals; D001835:Body Weight; D050260:Carbohydrate Metabolism; D002482:Cellulose / Q000494:pharmacology*; D004043:Dietary Fiber / Q000494:pharmacology*; D004435:Eating; D005243:Feces; D050356:Lipid Metabolism; D008099:Liver / Q000201:enzymology*; D051379:Mice; D009249:NADP / Q000378:metabolism
D004043:Dietary Fiber; D009249:NADP; D002482:Cellulose; C027433:bagasse