The putative transforming protein of S13 avian erythroblastosis virus is a transmembrane glycoprotein with an associated protein kinase activity.
S13 is an avian retrovirus that transforms both fibroblasts and erythroblasts. The gene product responsible for the oncogenic effects of S13 is the env-related glycoprotein gp155. In this report we show that gp155 is a transmembrane protein with a 55-kDa cytoplasmic domain. Pulse-chase analysis shows that gp155 was cleaved posttranslationally into two glycosylated proteins, gp85 and gp70. In addition, we show that a tyrosine protein kinase activity is associated only with the gp70 protein in microsomes and in immune complexes.
D001355:Alpharetrovirus / Q000235:genetics*; D000818:Animals; D001354:Avian Leukosis Virus / Q000235:genetics*; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D002645:Chickens; D006023:Glycoproteins / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D008970:Molecular Weight; D009856:Oncogene Proteins, Viral / Q000276:immunology / Q000502:physiology*; D011487:Protein Conformation; D011494:Protein Kinases / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D014759:Viral Envelope Proteins / Q000276:immunology / Q000502:physiology*
D006023:Glycoproteins; D008565:Membrane Proteins; D009856:Oncogene Proteins, Viral; D014759:Viral Envelope Proteins; D011494:Protein Kinases