Relationship between DNA acid-solubility and frequency of single-strand breaks near apurinic sites.
Using [32P]DNA alkylated with [3H]methyl methanesulfonate, depurinated by heating at 50 degrees C for various periods, then treated with sodium hydroxide, a table was constructed giving the DNA fraction soluble in 5% perchloric acid at 0 degree C as a function of the frequency of strand breaks. The alkaline treatment placed a break near each apurinic site; the apurinic sites were counted in two ways which gave consonant results: by the loss of [3H]methyl groups and by reaction with [14C]methoxyamine. The 32P label of DNA was used to measure the acid-solubility.
D004269:DNA, Bacterial / Q000378:metabolism*; D004926:Escherichia coli / Q000032:analysis; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D006898:Hydroxylamines / Q000378:metabolism; D008741:Methyl Methanesulfonate; D008745:Methylation; D010472:Perchlorates; D011687:Purines; D012972:Sodium Hydroxide; D012995:Solubility
D004269:DNA, Bacterial; D006898:Hydroxylamines; D010472:Perchlorates; D011687:Purines; D012972:Sodium Hydroxide; C005214:methoxyamine; D008741:Methyl Methanesulfonate