Chemotaxis of human neutrophils and eosinophils towards leukotriene B4 and its 20-w-oxidation products in vitro.
Peripheral blood neutrophils and eosinophils from 70 patients and controls were studied for their in vitro chemotactic and chemokinetic responses towards synthetic leukotriene B4 (LTB4), 20-OH-LTB4 and 20-COOH-LTB4. All three factors induced chemotaxis and chemokinesis of cells. 20-OH-LTB4 was always less and 20-COOH-LTB4 even less active than the parent compound. Cells from patients with atopic eczema and T cell lymphoma moved less than cells from normal controls or from patients with psoriasis. In the presence of LTB4, 20-OH-LTB4 and buffer alone, more eosinophils than neutrophils moved to the lower side of the filter, while this did not occur with platelet activating factor as chemoattractant. Studies of neutrophil and eosinophil chemotaxis in the presence of LTB4 should therefore always take into account a high variability of the quantitative response which is donor and disease dependent.
D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D003876:Dermatitis, Atopic / Q000097:blood; D004802:Eosinophilia / Q000097:blood; D004804:Eosinophils / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D006893:Hydroxyeicosatetraenoic Acids; D066298:In Vitro Techniques; D007975:Leukotriene B4 / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology*; D008223:Lymphoma / Q000097:blood; D009504:Neutrophils / Q000187:drug effects* / Q000502:physiology; D011565:Psoriasis / Q000097:blood
D006893:Hydroxyeicosatetraenoic Acids; D007975:Leukotriene B4; C031571:5,12,20-trihydroxy-6,8,10,14-eicosatetraenoic acid; C031911:20-carboxyleukotriene B4