title: Nerve dependent regulation of neural cell adhesion molecule expression in skeletal muscle.
abstract: The expression of neural cell adhesion molecule was analysed by indirect immunofluorescence on adult mouse skeletal muscle subjected to a variety of experimental lesions. Adult mouse muscle does not express neural cell adhesion molecule at the sarcolemma. However, following denervation there is a rapid rise in neural cell adhesion molecule levels; this is initially in the cytoplasm of the myofibres but by 18 days there is intense reactivity at the sarcolemma. A nerve crush lesion was used to show that the increase in neural cell adhesion molecule levels following denervation is accompanied by a switch-off of neural cell adhesion molecule expression following reinnervation. Paralysis of skeletal muscle by botulinum toxin injection is sufficient to activate neural cell adhesion molecule expression. As paralysis of skeletal muscle by botulinum toxin is not accompanied by activation of muscle satellite cells or degeneration products of nerve or myelin, it suggests that the observed levels of neural cell adhesion molecule are synthesised by myofibres. As the expression of neural cell adhesion molecule in these lesions parallels the ability of skeletal muscle to accept innervation it is possible that neural cell adhesion molecule acts as a molecular cue at the sarcolemma in regulating synaptogenesis.
mesh_terms: D000818:Animals; D000954:Antigens, Surface / Q000378:metabolism*; D001905:Botulinum Toxins / Q000494:pharmacology; D015815:Cell Adhesion Molecules; D005455:Fluorescent Antibody Technique; D051379:Mice; D008807:Mice, Inbred BALB C; D009121:Muscle Denervation; D009132:Muscles / Q000294:innervation / Q000378:metabolism*; D009416:Nerve Regeneration; D009469:Neuromuscular Junction / Q000502:physiology; D010525:Peripheral Nerves / Q000502:physiology*; D009435:Synaptic Transmission
chemical_list: D000954:Antigens, Surface; D015815:Cell Adhesion Molecules; D001905:Botulinum Toxins