title: Characterization of a new endoglucanase from Erwinia chrysanthemi.
abstract: The structural gene coding for a new endo-beta-1,4-glucanase of Erwinia chrysanthemi strain 3665, previously identified in a cosmid library, was subcloned into pUC18. The gene is expressed from a 1.9 X 10(3)-base-pair insert and its direction of transcription was determined. The properties of the gene product purified from cell-free extracts of Escherichia coli have been studied. The purified protein has an endoglucanase activity but is significantly different from the major endoglucanase Z secreted by E. chrysanthemi strain 3665. The new enzyme was designated as endoglucanase Y and the related gene celY. In E. coli, most of the endoglucanase activity was found in the periplasmic space.
mesh_terms: D002480:Cellulase / Q000235:genetics* / Q000378:metabolism; D004262:DNA Restriction Enzymes; D004885:Erwinia / Q000201:enzymology* / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005796:Genes; D005798:Genes, Bacterial; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D014158:Transcription, Genetic
chemical_list: D004262:DNA Restriction Enzymes; D002480:Cellulase