title: Maintenance of autonomous genetic elements in twelve transgenic mouse strains established after transfer of pPyLT1 DNA.
abstract: Maintenance and efficient meiotic segregation of autonomous genetic elements in four transgenic mouse strains established after micro-injection of plasmid pPyLT1 were previously reported. These findings are now extended to a total of twelve independent transgenic families. In spite of extensive rearrangements, half of these plasmids maintained the region of pBR322 necessary for shuttle transfer in E. coli. They all included mouse DNA sequences and the same, or closely related sequences were present in distinct mouse strains.
mesh_terms: D000818:Animals; D004247:DNA / Q000235:genetics; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D008845:Microinjections; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D011120:Polyomavirus / Q000235:genetics; D014162:Transfection; D014170:Transformation, Genetic; D015053:Zygote
chemical_list: D004274:DNA, Recombinant; D004247:DNA