title: Outer membrane protein mediating iron uptake via pyoverdinpss, the fluorescent siderophore produced by Pseudomonas syringae pv. syringae.
abstract: In an iron-limited environment Pseudomonas syringae pv. syringae B301D produces a yellow-green fluorescent siderophore called pyoverdinpss which functions in high-affinity iron transport. Two-dimensional electrophoretic comparisons of the outer membrane proteins of strain B301D identified nine proteins which were expressed at low (50 nM) but not at high (10 microM) iron concentrations. Except for the minor protein 8e, the iron-regulated proteins exhibited high molecular weights ranging from approximately 74,000 to 80,000. A mutant of strain B301D incapable of iron uptake (Iu-) from ferric pyoverdinpss lacked the 74,000-molecular-weight protein 4a, which was the major iron-regulated outer membrane protein. In contrast, a nonfluorescent mutant (Flu-) unable to synthesize pyoverdinpss showed no quantitative or qualitative difference in its outer membrane profile from that of the wild-type strain. In plant pathogenicity tests the Iu- and Flu- strains caused typical brown necrotic and sunken lesions in immature sweet cherry fruit which were indistinguishable from those of the wild-type strain. Thus, excretion of pyoverdinpss and subsequent Fe(III) uptake do not have a determinative role in the pathogenicity or virulence of P. syringae pv. syringae.
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism*; D007501:Iron / Q000378:metabolism*; D007526:Isoelectric Point; D008970:Molecular Weight; D009154:Mutation; D009842:Oligopeptides; D010860:Pigments, Biological / Q000378:metabolism*; D010935:Plant Diseases; D011549:Pseudomonas / Q000254:growth & development / Q000378:metabolism* / Q000472:pathogenicity; D011956:Receptors, Cell Surface / Q000378:metabolism*
chemical_list: D001425:Bacterial Outer Membrane Proteins; D009842:Oligopeptides; D010860:Pigments, Biological; D011956:Receptors, Cell Surface; C035902:siderophore receptors; C042453:pyoverdin; D007501:Iron