[Effectiveness of incorporation of spermine-condensed viral DNA into liposomes. Interaction of liposomes with cells].
DNA from bacteriophage lambda or monkey adenovirus type 7 have been condensed with spermine and included into three types of liposomes: ethereal, obtained by inverted phases technique and by Ca++ fusion. Compact DNA form presents a tor with 100 nm external diameter and 430 nm width. It can be included into 100 nm or larger liposomes. Total preparation contains 15-18% of the required liposomes. Liposomal fractions with included DNA were separated from empty liposomes by step gradient of ficoll 400. Liposomal fraction having included DNA contains 15-18% of common lipid. Liposomal interaction with monkey cell cultures has been studied.
D000818:Animals; D010582:Bacteriophage lambda / Q000032:analysis; D002460:Cell Line; D002522:Chlorocebus aethiops; D004279:DNA, Viral / Q000032:analysis / Q000187:drug effects*; D008081:Liposomes / Q000032:analysis*; D009690:Nucleic Acid Conformation; D013539:Simian virus 40 / Q000032:analysis; D013096:Spermine / Q000494:pharmacology*
D004279:DNA, Viral; D008081:Liposomes; D013096:Spermine
Effektivnost' vkliucheniia kondensirovannykh sperminon virusnykh DNK v liposomy. Vzaimodeĭstvie liposom s kletkami.