Dot immunobinding assay of viral antigen and antibodies.
A dot-immunobinding assay for the rapid and specific detection of viral antibody as well as the identification of a virus is described. Based on the use of nitrocellulose membranes for the adsorption of viral protein, this test may be used to detect the presence of virus antibody or identify an isolate within 4 to 6 hours. Use of goat anti-human IgG-alkaline phosphatase followed by a naphthol-AS-MX-phosphate: Fast Red substrate permits visual detection of a positive reaction. The use of psoralen inactivated herpes B virus permits the incorporation of this virus into the battery of test antigens.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D000956:Antigens, Viral / Q000032:analysis*; D006566:Herpesviridae Infections / Q000175:diagnosis; D006801:Humans; D007163:Immunosorbent Techniques; D008253:Macaca mulatta; D002679:Pan troglodytes; D010215:Papio; D014764:Viral Proteins / Q000032:analysis
D000914:Antibodies, Viral; D000956:Antigens, Viral; D014764:Viral Proteins