title: Motoneurone survival and neurite regeneration requirements: the role of dorsal root ganglion cells during development.
abstract: The effect of dissociated dorsal root ganglion cells on the survival of motoneurones in vitro from differently aged chick embryos has been studied. Homogeneous cultures of motoneurones were prepared from 5-8-day embryos, using a cell sorter; dorsal root ganglion cells were obtained from 8-day embryos. The survival of motoneurones from 5-day and 6-day embryos was not enhanced above controls by the presence of dorsal root ganglion cells; however, the survival of motoneurones from older embryos was greatly increased, reaching a maximum of over 80% for 8-day embryonic motoneurones. In contrast, the number of motoneurones that had regenerated neurites when co-cultured with dorsal root ganglion cells for 24 h decreased with the motoneurone age at plating, from 51% at 5 days to less than 10% for 7- and 8-day motoneurones. The survival-enhancing effects were probably mediated by cell contact between the motoneurones and processes of the dorsal root ganglion cells: conditioned media from high-density cultures of dorsal root ganglion cells could not be shown to significantly enhance the survival of motoneurones above that of control levels. The possibility that the ganglion cells exert this survival enhancing effect by depolarizing the motoneurones was examined by exposing 8-day sorted motoneurones to 47 mM potassium; this did not effect the survival of the motoneurones relative to control levels. The stage dependency of the survival of motoneurones on different neurotrophic factors and the dorsal root ganglion cell is discussed.
mesh_terms: D000818:Animals; D002450:Cell Communication; D002470:Cell Survival; D002478:Cells, Cultured; D002642:Chick Embryo; D005727:Ganglia, Spinal / Q000196:embryology*; D009046:Motor Neurons / Q000166:cytology*; D009414:Nerve Growth Factors / Q000494:pharmacology; D013116:Spinal Cord / Q000196:embryology*; D013569:Synapses / Q000502:physiology; D009435:Synaptic Transmission
chemical_list: D009414:Nerve Growth Factors