title: Cholesteryl esterase activities in ventricles, isolated heart cells and aorta of the rat.
abstract: Cholesteryl esterase activities were determined in homogenates of rat heart (ventricles), isolated, calcium-tolerant, cardiac myocytes and aortic tissue and were compared with acid and neutral triglyceride lipase activities in these fractions. Using cholesteryl oleate/phosphatidylcholine/taurocholate emulsions and digitonin pretreatment of the enzyme fractions, acid and neutral cholesteryl esterase activities were measured in all tissue preparations. In contrast to the acid and neutral triglyceridase and acid cholesteryl esterase activity, the neutral cholesteryl esterase activity was subject to substrate inhibition. Upon isolation of cardiac myocytes, and in contrast with the recovery of neutral triglyceride lipase activity, only a small portion of the neutral cholesteryl esterase (6%) was recovered, suggesting that nonmyocyte neutral cholesteryl esterase activity markedly contributes to the relatively high activity detectable in whole ventricular homogenates. The recovery of large amounts of neutral cholesteryl esterase activity in the supernatant of collagenase-digested heart tissue, obtained during the isolation of myocytes, which is also markedly enriched in activities of two endothelial marker enzymes (5'-nucleotidase and angiotensine-converting enzyme) may indicate the predominant contribution of neutral cholesteryl esterase activity from coronary endothelial cells to this activity detectable in ventricular homogenates. Relative to the activity in ventricular and myocyte homogenates, aorta homogenates possessed the highest specific neutral cholesteryl esterase activity. We propose that in addition to coronary endothelium, smooth muscle cells also contribute to the neutral cholesteryl esterase activity in ventricular homogenates.(ABSTRACT TRUNCATED AT 250 WORDS)
mesh_terms: D015720:5'-Nucleotidase; D000818:Animals; D001011:Aorta / Q000201:enzymology*; D002118:Calcium / Q000494:pharmacology; D002265:Carboxylic Ester Hydrolases / Q000032:analysis*; D002351:Carrageenan / Q000494:pharmacology; D006352:Heart Ventricles; D006863:Hydrogen-Ion Concentration; D008049:Lipase / Q000032:analysis; D008297:Male; D009206:Myocardium / Q000201:enzymology*; D009708:Nucleotidases / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D002787:Sterol Esterase / Q000032:analysis*
chemical_list: D002351:Carrageenan; D002265:Carboxylic Ester Hydrolases; D002787:Sterol Esterase; D008049:Lipase; D009708:Nucleotidases; D015720:5'-Nucleotidase; D002118:Calcium