VSV replication in normal and transformed T cells, an assay for T suppressor cell activation.
An infectious center viral plaque assay has been utilized to quantitate activated T suppressor (Ts) cells. This assay is based on two observations. Namely, resting T cells do not serve as good replicative hosts for many viruses, including vesicular stomatitis virus (VSV), and that Ts cells can be enriched by their ability to bind to antigen-coated dishes. Our data show that Ts cells specific for either the TNP hapten or for dextran will replicate VSV upon antigenic and/or mitogenic activation, whereas resting Ts and hapten-specific B cells are less efficient in this process. This system will now allow the direct quantitation of Ts cells and their activation properties.
D000818:Animals; D000941:Antigens / Q000276:immunology; D002472:Cell Transformation, Viral; D008213:Lymphocyte Activation; D008222:Lymphokines / Q000494:pharmacology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D050378:T-Lymphocytes, Regulatory / Q000276:immunology* / Q000382:microbiology; D014721:Vesicular stomatitis Indiana virus / Q000502:physiology*; D014779:Virus Replication
D000941:Antigens; D008222:Lymphokines