Beta-1,3-glucan receptor and peptidoglycan receptor are present as separate entities within insect prophenoloxidase activating system.
Silkworm plasma was passed over a peptidoglycan-Sepharose 4B column or a CPB column [CPB, curdlan type polysaccharide (beta-1,3-glucan) bead] in the absence of divalent cation and the effluents from the columns were named plasma-PG and plasma-CPB, respectively. Prophenoloxidase activating system in plasma-PG was triggered by beta-1,3-glucan but not by peptidoglycan and the system in plasma-CPB was triggered by peptidoglycan but not by beta-1,3-glucan, suggesting that the peptidoglycan-Sepharose 4B column and the CPB column remove peptidoglycan-receptor and beta-1,3-glucan-receptor, respectively, from plasma. This result indicates that both receptors exist as separate entities in silkworm plasma. It is suggested that plasma-PG and plasma-CPB may be used as specific reagents to detect minute amounts of beta-1,3-glucan and peptidoglycan.
D000818:Animals; D012831:Bombyx / Q000201:enzymology*; D004156:Catechol Oxidase / Q000378:metabolism*; D002846:Chromatography, Affinity; D004789:Enzyme Activation / Q000187:drug effects; D004792:Enzyme Precursors / Q000378:metabolism*; D005936:Glucans / Q000494:pharmacology; D010457:Peptidoglycan / Q000494:pharmacology; D011956:Receptors, Cell Surface / Q000302:isolation & purification / Q000502:physiology*; D011971:Receptors, Immunologic; D047071:beta-Glucans
D004792:Enzyme Precursors; D005936:Glucans; D010457:Peptidoglycan; D011956:Receptors, Cell Surface; D011971:Receptors, Immunologic; D047071:beta-Glucans; C045268:beta-glucan receptor; C051223:peptidoglycan receptor; C033363:beta-1,3-glucan; C038451:pro-phenoloxidase; D004156:Catechol Oxidase