[Demonstration and classification of heterogeneity of phosphoprotein phosphohydrolase in swine spleen extracts based on sodium fluoride inhibition].
The heterogeneity of phosphoprotein phosphohydrolase--Fpf in cellular free spleen extracts of pigs may be determined by sodium fluoride inhibition. NaF concentration of 5 X 10(-3) mol/l divides Fpf into 3 groups and shows a wide inhibition range from 15 to 85% of the initial activity of the enzyme. Naf concentration of 5 X 10(-4)mol/l stops Fpf activity in the range from 10 to 65% and divides it into two groups. To classify the studied material into Fpf phenotypes, fluoride quotient index--WIF has been proposed which expresses the relationship between the percentage of the inhibition caused by NaF at a concentration 5 X 10(-3) mol/l and that of the inhibition caused by NaF at a concentration of 5 X 10(-4) mol/l in the reaction mixture and in the measurement conditions described in the methods. Five groups with the following numerical values of WIF have been distinguished: 1.15 less than or equal to WIF less than 1.40-44%--group I; 1.50 less than or equal to WIF less than 1.80-20%--group II; 1.90 less than or equal to WIF less than 2.10-16%--group III; 2.20 less than or equal to WIF less than 2.50-16%--group IV; and WIF = 3-4%--group V.
D000818:Animals; D007527:Isoenzymes / Q000145:classification / Q000302:isolation & purification; D008970:Molecular Weight; D010641:Phenotype; D010749:Phosphoprotein Phosphatases / Q000037:antagonists & inhibitors / Q000145:classification / Q000302:isolation & purification*; D012969:Sodium Fluoride / Q000494:pharmacology; D013154:Spleen / Q000201:enzymology*; D013552:Swine; D014020:Tissue Extracts / Q000032:analysis
D007527:Isoenzymes; D014020:Tissue Extracts; D012969:Sodium Fluoride; D010749:Phosphoprotein Phosphatases
Próba wykazania i klasyfikacji heterogenności fosfohydrolazy fosfoproteidów w surowych ekstraktach śledzion świń na podstawie inhibicji fluorkiem sodu.