title: [Insertion mutagenesis in Bacillus subtilis (Marmur)].
abstract: A method for obtaining mutations in Bacillus subtilis using integration into the chromosome of the plasmid pHV60 ligated to chromosomal DNA fragments was developed. Auxotrophic mutants acquire, in addition, chloramphenicol-resistance, due to insertion of appropriate plasmid determinants. Chromosomal localization was established and the properties of several mutants were studied.
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics*; D004251:DNA Transposable Elements; D005798:Genes, Bacterial; D009154:Mutation; D010957:Plasmids; D014169:Transformation, Bacterial
chemical_list: D004251:DNA Transposable Elements
vernacular_title: Izuchenie insertsionnogo mutageneza u Bacillus subtilis (Marmur).