Interaction of strain AD169 and a clinical isolate of cytomegalovirus with peripheral monocytes: the effect of lipopolysaccharide stimulation.
The effect of human cytomegalovirus (CMV) infection on lipopolysaccharide (LPS)-stimulated and unstimulated monocytes from seronegative donors was studied by using the laboratory-adapted strain AD169 and a recent clinical isolate. LPS-stimulated and unstimulated monocytes infected with the isolate showed expression of immediate-early CMV antigens and were significantly more suppressive for lymphocyte proliferation than were strain AD169-infected monocytes, which rarely expressed detectable viral protein. Human CMV infection of LPS-stimulated and unstimulated monocytes resulted in abrogation of interleukin-1 activity, with the effect being marked in LPS-stimulated monocytes infected with the clinical isolate of CMV. Addition of interleukin-1 to infected, stimulated monocytes completely restored lymphoproliferative responses to phytohemagglutinin, whereas addition of this leukokine to infected, unstimulated cells could not restore this response.
D000328:Adult; D000956:Antigens, Viral / Q000032:analysis; D002478:Cells, Cultured; D003587:Cytomegalovirus / Q000276:immunology / Q000502:physiology*; D006801:Humans; D017874:Immediate-Early Proteins; D007375:Interleukin-1 / Q000096:biosynthesis / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008213:Lymphocyte Activation; D008875:Middle Aged; D009000:Monocytes / Q000276:immunology / Q000382:microbiology*; D013601:T-Lymphocytes / Q000276:immunology; D014779:Virus Replication
D000956:Antigens, Viral; D017874:Immediate-Early Proteins; D007375:Interleukin-1; D008070:Lipopolysaccharides; C044582:immediate-early proteins, cytomegalovirus