Heparin-binding angiogenesis factors: detection by immunological methods.
Immunological methods for the detection of basic fibroblast growth (FGF) are described. Polyclonal antibodies directed against synthetic peptides representing amino-, internal, and carboxy-terminal regions of basic FGF, were raised in rabbits. Five techniques, enzyme-linked immunosorbent assay, immunoblot, electrophoretic transfer (Western) blot, immunoprecipitation and radioimmunoassay, were used to detect basic FGF. These techniques were used to demonstrate that a human hepatoma cell line synthesizes a growth factor structurally related to brain and pituitary basic FGF.
D043925:Angiogenesis Inducing Agents / Q000302:isolation & purification*; D000906:Antibodies; D006528:Carcinoma, Hepatocellular; D002460:Cell Line; D005346:Fibroblast Growth Factors / Q000302:isolation & purification; D006133:Growth Substances / Q000302:isolation & purification*; D006493:Heparin / Q000378:metabolism; D006801:Humans; D007118:Immunoassay / Q000379:methods; D008113:Liver Neoplasms
D043925:Angiogenesis Inducing Agents; D000906:Antibodies; D006133:Growth Substances; D005346:Fibroblast Growth Factors; D006493:Heparin