[Purification and substrate specificity of BcmI restriction endonuclease].
A strain producing the new specific restriction endonuclease BcmI has been found in the Bacillus generum. The enzyme has been purified by chromatography on the blue sepharose, phosphocellulose PII, heparin sepharose. The analogous purification has been obtained when the blue sepharose has been substituted for the orange sepharose, the home produced sorbent. The BcmI enzyme has been shown by the substrate specificity definition to be an isoschizomer of the restriction endonuclease ClaI.
D001407:Bacillus / Q000201:enzymology*; D001483:Base Sequence; D002852:Chromatography, Ion Exchange; D004262:DNA Restriction Enzymes / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific; D004588:Electrophoresis, Cellulose Acetate; D013379:Substrate Specificity
D004262:DNA Restriction Enzymes; C032824:ATCGAT-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific
Ochistka i opredelenie substratnoĭ spetsifichnosti restriktsionnoĭ éndonukleazy BcmI.