Effects of adenosine and adenosine analogues on glycogen metabolism in isolated rat hepatocytes.
Adenosine and adenosine analogues were incubated with isolated rat hepatocytes. Adenosine and 5'-deoxy-5'-chloroadenosine stimulated glucose release, glycogen loss, and the conversion of glycogen phosphorylase b to a. The effect was of short duration for adenosine, but of long duration for 5'-deoxy-5'-chloroadenosine. The effects on glucose release and phosphorylase were blocked by theophylline, an R-receptor blocking agent, but not by nitrobenzylthioinosine or dipyridamol which are nucleoside transport inhibitors. A dose-dependent rise in cyclic AMP concentration was observed in hepatocytes 1 min after adding adenosine. It is concluded that adenosine exerts these effects in liver by activating adenylcyclase. Adenosine may be involved in the short-term regulation of hepatic glycogen phosphorylase.
D000241:Adenosine / Q000494:pharmacology*; D000818:Animals; D017338:Cladribine; D000242:Cyclic AMP / Q000378:metabolism; D003839:Deoxyadenosines / Q000031:analogs & derivatives* / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D005947:Glucose / Q000378:metabolism; D006003:Glycogen / Q000378:metabolism*; D007700:Kinetics; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D010762:Phosphorylase a / Q000378:metabolism; D010763:Phosphorylase b / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013806:Theophylline / Q000494:pharmacology
D003839:Deoxyadenosines; D017338:Cladribine; C044739:5'-chloro-5'-deoxyadenosine; D006003:Glycogen; D013806:Theophylline; D000242:Cyclic AMP; D010762:Phosphorylase a; D010763:Phosphorylase b; D005947:Glucose; D000241:Adenosine