title: A single gene produces multiple sericin messenger RNAs in the silk gland of Bombyx mori.
abstract: The sericins are a family of major cocoon proteins specifically synthesized in the middle silk gland of the silkworm Bombyx mori. The 5' part of one sericin gene had been cloned and described by Okamoto et al. (1982, J. Biol. Chem. 257, 15192-15199). Using a differential screening procedure of Bombyx genomic libraries, we obtained the 3' part of this gene. We demonstrate that it consists of a single gene extending over 24 kb, present in two allelic forms in hybrid strains. This gene encodes for four mRNAs which result from a unique transcript by an alternative splicing mechanism. This explains, at least partially, the diversity of the sericins found in the cocoon.
mesh_terms: D001483:Base Sequence; D012831:Bombyx / Q000235:genetics*; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant; D005796:Genes; D009693:Nucleic Acid Hybridization; D010456:Peptides, Cyclic / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D047030:Sericins; D012694:Serine / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D004274:DNA, Recombinant; D010456:Peptides, Cyclic; D012333:RNA, Messenger; D047030:Sericins; D012694:Serine; D004262:DNA Restriction Enzymes