Glucose regulates preproinsulin messenger RNA levels in a clonal cell line of simian virus 40-transformed B cells.
In HIT-T15 cells grown in the absence of glucose, Northern blot analysis of total RNA revealed a major 0.5 kb preproinsulin (ppI) mRNA transcript which co-migrated with the mature transcript from a human insulinoma. In 4 h tissue cultures, glucose (2-20 mM) stimulated HIT cell ppI mRNA levels in a markedly dose-dependent manner. Glucose-stimulated ppI mRNA was (i) inhibited by actinomycin D, suggesting that regulation may be in part transcriptional, and (ii) potentiated by agents known to activate B cell protein kinases. HIT cells represent a unique model for investigating long term regulation of insulin gene expression and biosynthesis.
D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism*; D001839:Bombesin / Q000494:pharmacology; D002460:Cell Line; D002472:Cell Transformation, Viral; D002999:Clone Cells; D005576:Colforsin / Q000494:pharmacology; D006224:Cricetinae; D003609:Dactinomycin / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005947:Glucose / Q000494:pharmacology*; D007328:Insulin; D008647:Mesocricetus; D011384:Proinsulin / Q000235:genetics*; D011494:Protein Kinases / Q000378:metabolism; D011498:Protein Precursors / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism*; D013539:Simian virus 40; D013806:Theophylline / Q000494:pharmacology
D007328:Insulin; D011498:Protein Precursors; D012333:RNA, Messenger; D003609:Dactinomycin; D005576:Colforsin; C013737:preproinsulin; D011384:Proinsulin; D013806:Theophylline; D011494:Protein Kinases; D005947:Glucose; D001839:Bombesin