Immunofluorescent staining of kinetochores in micronuclei: a new assay for the detection of aneuploidy.
The immunofluorescent staining of kinetochores in micronuclei with antikinetochore antibodies was used to develop an in vitro assay for aneuploidy-inducing agents. The results show that about 80% of micronuclei induced by either colchicine or chloral hydrate contained kinetochores; only 9% of X-ray-induced micronuclei reacted positively to the antibody. These findings indicate that the in vitro micronucleus assay coupled with immunofluorescent staining of kinetochores can be a useful method for assessing the ability of chemicals to induce aneuploidy and/or chromosome aberrations.
D000782:Aneuploidy; D000818:Animals; D001323:Autoantibodies; D002460:Cell Line; D002467:Cell Nucleus / Q000648:ultrastructure; D002697:Chloral Hydrate / Q000494:pharmacology; D002869:Chromosome Aberrations; D002875:Chromosomes / Q000648:ultrastructure*; D003078:Colchicine / Q000494:pharmacology; D006224:Cricetinae; D005455:Fluorescent Antibody Technique; D009152:Mutagenicity Tests; D014965:X-Rays
D001323:Autoantibodies; D002697:Chloral Hydrate; D003078:Colchicine