Effect of internal viral sequences on the utility of retroviral vectors.
Expression of the human ADA cDNA encoded by the Moloney murine leukemia virus spliced RNA form is enhanced by intron-contained sequences. The presence of sequences corresponding to the viral gag gene in a Moloney murine leukemia virus-based vector results in the generation of 10- to 40-fold higher titers of virus.
D000243:Adenosine Deaminase / Q000235:genetics; D005822:Genetic Vectors; D019868:Kanamycin Kinase; D009052:Leukemia Virus, Murine / Q000235:genetics; D010770:Phosphotransferases / Q000235:genetics; D012326:RNA Splicing; D012091:Repetitive Sequences, Nucleic Acid; D012190:Retroviridae / Q000235:genetics*; D012641:Selection, Genetic
D010770:Phosphotransferases; D019868:Kanamycin Kinase; D000243:Adenosine Deaminase