title: Release of leukotriene B4 from sublethally injured oligodendrocytes by terminal complement complexes.
abstract: In the present study, the interaction of the terminal complement complexes with oligodendrocytes was investigated for observation of its effect on membrane lipid hydrolysis. [14C]Arachidonic acid was incorporated into the membrane lipids of cultured oligodendrocytes before sensitization with anti-galactocerebroside antiserum. Cells were then exposed to excess C6-deficient rabbit serum reconstituted with limiting doses of C6 to form various numbers of C5b-9 complexes. Qualitative analysis of the supernatants by HPLC revealed the presence of compounds that coeluted with arachidonic acid and its oxygenated derivatives, prostaglandin E2, leukotrienes E4 and B4, and 15-hydroxyeicosatetraenoic acid. The kinetics of leukotriene B4 release by excess C5b-8 was quantitated by radioimmunoassay. Leukotriene B4 release approached a maximum around 30 min, and C6 dose-response studies performed at 1 h showed that maximal levels of leukotriene B4 were detected over a range of sublytic C5b-9 attack. Maximal release of leukotriene B4 was also achieved by C5b-8 without further enhancement by addition of lytic doses of C9. Results indicate that sublytic attack of oligodendrocytes by complement induces release of lipid-derived inflammatory mediators.
mesh_terms: D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism; D001921:Brain / Q000166:cytology; D002462:Cell Membrane / Q000276:immunology / Q000378:metabolism; D002470:Cell Survival; D003182:Complement C5 / Q000276:immunology; D050776:Complement C5b; D003183:Complement C6 / Q000276:immunology; D003186:Complement C9 / Q000276:immunology; D003165:Complement System Proteins / Q000276:immunology*; D007700:Kinetics; D007975:Leukotriene B4 / Q000276:immunology*; D008563:Membrane Lipids / Q000378:metabolism; D009457:Neuroglia / Q000276:immunology*; D009836:Oligodendroglia / Q000276:immunology* / Q000378:metabolism; D051381:Rats
chemical_list: D001095:Arachidonic Acids; D003182:Complement C5; D003183:Complement C6; D003186:Complement C9; D008563:Membrane Lipids; D007975:Leukotriene B4; D016718:Arachidonic Acid; D050776:Complement C5b; D003165:Complement System Proteins