Functional evaluation of glutamate receptor subtypes in cultured cerebellar neurones and astrocytes.
Micromolar concentrations of kainic acid and quisqualic acid released [3H]D-aspartate preaccumulated by cerebellar granule cells in culture. The effect of kainate was selectively antagonized by kynurenic acid and, less effectively, by PDA. Kainate and quisqualate also increased [3H]GABA release from a subpopulation of cultured cerebellar astrocytes. Kynurenic acid selectively blocked the effect of kainic acid. NMDA and no D-aspartate or GABA releasing effect. These results suggest the existence of two different excitatory amino acid sites active on neurotransmitter amino acid release in both cerebellar granule cells and astrocytes.
D000596:Amino Acids / Q000627:therapeutic use; D000598:Amino Acids, Cyclic; D000818:Animals; D001253:Astrocytes / Q000378:metabolism*; D002478:Cells, Cultured; D002531:Cerebellum / Q000378:metabolism*; D003509:Cyclohexanecarboxylic Acids / Q000627:therapeutic use; D007608:Kainic Acid / Q000494:pharmacology; D009474:Neurons / Q000378:metabolism*; D010069:Oxadiazoles / Q000494:pharmacology; D016318:Quisqualic Acid; D051381:Rats; D017470:Receptors, Glutamate; D017981:Receptors, Neurotransmitter / Q000378:metabolism*
D000596:Amino Acids; D000598:Amino Acids, Cyclic; D003509:Cyclohexanecarboxylic Acids; D010069:Oxadiazoles; D017470:Receptors, Glutamate; D017981:Receptors, Neurotransmitter; C015666:3-aminocyclohexanecarboxylic acid; D016318:Quisqualic Acid; D007608:Kainic Acid