[An ultrastructural study of the localization of alkaline phosphatase and Na-K ATPase in the labyrinth from the IUGR rat].
To elucidate the pathogenesis of intrauterine growth retardation (IUGR), the ischemic placentae produced by uterine artery and vein ligation were morphologically and histochemically investigated in rats. In the ischemic condition, fetal growth was remarkably retarded. The labyrinthi were atrophic and trophoblasts were degenerated ultrastructurally in association with mitochondrial swelling, increased microvesicles, dense bodies and fibrin deposition in the dilated intercellular spaces. Alkaline phosphatase and Na-K ATPase were localized on the plasma membrane in control rats. Na-K ATPase activity was observed in the basal side of cytotrophoblasts. However, the enzyme activities were decreased in the ischemic condition. The reaction products of horseradish peroxidase were present in the dilated intercellular spaces and increased cytoplasmic vesicles. These results suggest that in the ischemic condition, IUGR might be induced by the plasma membrane dysfunction following decreased enzyme activities and irregular distribution of horseradish peroxidase.
D000469:Alkaline Phosphatase / Q000032:analysis*; D000818:Animals; D002824:Chorionic Villi / Q000201:enzymology* / Q000648:ultrastructure; D005260:Female; D005317:Fetal Growth Retardation / Q000201:enzymology* / Q000473:pathology; D011247:Pregnancy; D051381:Rats; D011919:Rats, Inbred Strains; D000254:Sodium-Potassium-Exchanging ATPase / Q000032:analysis*
D000469:Alkaline Phosphatase; D000254:Sodium-Potassium-Exchanging ATPase