title: Thiamine triphosphate and membrane-associated thiamine phosphatases in the electric organ of Electrophorus electricus.
abstract: The main electric organ of Electrophorus electricus is particularly rich in thiamine triphosphate, which represents 87% of the total thiamine content in this tissue. The thiamine pyrophosphate concentration, however, is very low in the eel electric organ and skeletal muscle as compared with other eel or rat tissues. Furthermore, electroplax membranes contain a whole set of enzymes responsible for the dephosphorylation of thiamine tri-, pyro- and monophosphate. Thiamine triphosphatase has a pH optimum of 6.8 and is dependent on Mg2+. The real substrate of the enzyme is probably a 1:1 complex of Mg2+ and thiamine triphosphate. Thiamine pyrophosphatase is activated by Ca2+. The apparent Km for thiamine triphosphate and Vmax are found to be, respectively, 1.76 mM and 5.95 nmol/mg of protein/min. Thiamine triphosphatase activity is inhibited at physiological K+ concentrations (up to 90 mM) and increasing Na+ concentrations (50% inhibition at 300 mM). ZnCl2 (10 mM) inhibits 90% of the enzyme activity. ATP and ITP are also strongly inhibitory. No significant effect of neurotoxins is seen. Membrane-associated thiamine triphosphatase is affected differently by proteolytic enzymes and is partially inactivated by pretreatment with phospholipase C and neuraminidase. The physiological significance of thiamine triphosphatase is discussed in relation to a specific role of thiamine in the nervous system.
mesh_terms: D000818:Animals; D004557:Electric Organ / Q000032:analysis* / Q000201:enzymology; D004593:Electrophorus; D007700:Kinetics; D008566:Membranes / Q000201:enzymology; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*; D011755:Pyrophosphatases / Q000378:metabolism*; D013045:Species Specificity; D013837:Thiamin-Triphosphatase / Q000378:metabolism*; D013831:Thiamine / Q000031:analogs & derivatives* / Q000032:analysis*; D013833:Thiamine Monophosphate / Q000032:analysis; D013834:Thiamine Pyrophosphatase / Q000378:metabolism*; D013835:Thiamine Pyrophosphate / Q000032:analysis; D013838:Thiamine Triphosphate / Q000032:analysis*; D014018:Tissue Distribution
chemical_list: D013838:Thiamine Triphosphate; D013833:Thiamine Monophosphate; D010744:Phosphoric Monoester Hydrolases; D011755:Pyrophosphatases; D013834:Thiamine Pyrophosphatase; D013837:Thiamin-Triphosphatase; D013835:Thiamine Pyrophosphate; D013831:Thiamine