Temperature-dependent cytocidal effects of Moloney murine sarcoma virus.
Infection of L6E9 rat myoblasts with Moloney murine sarcoma virus (Mo-MuSV) at 37 degrees C resulted in the killing of target cells in less than one week. However, cells infected at 37 degrees C and shifted to 40 degrees C were not killed but underwent morphological transformation and had reduced levels of virus-specific RNA. A marked decrease in a cellular 55 kDa protein, as judged by immunoblotting, correlated with increased expression of Mo-MuSV RNA in infected cells.
D000818:Animals; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D008980:Moloney murine sarcoma virus / Q000235:genetics* / Q000254:growth & development; D009132:Muscles; D014176:Protein Biosynthesis; D051381:Rats; D009053:Sarcoma Viruses, Murine / Q000235:genetics*; D013696:Temperature; D014158:Transcription, Genetic