Context-dependent gene expression: cis-acting negative effects of specific procaryotic plasmid sequences on eucaryotic genes.
A sequence element within pBR322 DNA mediates a cis-acting negative effect on expression from eucaryotic genes in transient expression assays. The negative element overlaps with sequences that inhibit DNA replication, but its effect is observed in the absence of detectable replication of transfected DNA.
D000123:Acetyltransferases / Q000235:genetics; D000818:Animals; D015500:Chloramphenicol O-Acetyltransferase; D004261:DNA Replication; D005786:Gene Expression Regulation; D005796:Genes; D005798:Genes, Bacterial; D005814:Genes, Viral; D007739:L Cells; D008324:Mammary Tumor Virus, Mouse / Q000235:genetics; D051379:Mice; D010957:Plasmids; D011401:Promoter Regions, Genetic
D000123:Acetyltransferases; D015500:Chloramphenicol O-Acetyltransferase