c-myc protein can be substituted for SV40 T antigen in SV40 DNA replication.
Replicating activity of SV40 origin-containing plasmid was tested in human cells as well as in monkey CosI cells. All the plasmids possessing SV40 ori sequences could replicate, even in the absence of SV40 T antigen, in human HL-60 and Raji cells which are expressing c-myc gene at high level. The copy numbers of the replicated plasmids in these human cells were 1/100 as high as in monkey CosI cells which express SV40 T antigen constitutively. Exactly the same plasmids as the transfected original ones were recovered from the Hirt supernatant of the transfected HL-60 cells. Furthermore, replication of the SV40 ori-containing plasmids in HL-60 cells was inhibited by anti-c-myc antibody co-transfected into the cells. These results indicate that the c-myc protein can be substituted for SV40 T antigen in SV40 DNA replication.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000952:Antigens, Polyomavirus Transforming; D000957:Antigens, Viral, Tumor / Q000502:physiology*; D002460:Cell Line; D002522:Chlorocebus aethiops; D004261:DNA Replication / Q000187:drug effects*; D004274:DNA, Recombinant / Q000096:biosynthesis; D004279:DNA, Viral / Q000096:biosynthesis / Q000235:genetics; D004268:DNA-Binding Proteins / Q000494:pharmacology*; D006801:Humans; D009856:Oncogene Proteins, Viral / Q000502:physiology*; D010957:Plasmids; D011518:Proto-Oncogene Proteins / Q000276:immunology / Q000494:pharmacology*; D016271:Proto-Oncogene Proteins c-myc; D011994:Recombinant Proteins / Q000494:pharmacology; D013539:Simian virus 40 / Q000235:genetics*; D014162:Transfection
D000911:Antibodies, Monoclonal; D000952:Antigens, Polyomavirus Transforming; D000957:Antigens, Viral, Tumor; D004274:DNA, Recombinant; D004279:DNA, Viral; D004268:DNA-Binding Proteins; D009856:Oncogene Proteins, Viral; D011518:Proto-Oncogene Proteins; D016271:Proto-Oncogene Proteins c-myc; D011994:Recombinant Proteins