[Nitrogen exchange in the large intestine of ruminants. 4. Exchange and isotope balance of intracecally administered 14C- and 15N-urea in sheep with simultaneous intracecal dose of a partly hydrolyzed straw meal].
Two experiments were performed with wethers (Body weight 34 to 44 kg) receiving a ration rich in crude fibre at maintenance level. The animals were fitted with ileocaecal cannulas into which 14C-, 15N-labelled urea together with digesta was introduced hourly for a 24 hours period (V1; 2 animals). In experiment two (V2; 3 animals) in addition HCl-partly hydrolysed straw meal was introduced. After ureolytic degradation the intracaecal applied urea entered mainly the intermediary metabolism. The resulting ammonia was resynthesized to urea without any time lag. The rate constant for the increase in 15N labelling of urea was 3.2 d-1 in both experiments. Urea leaves the plasma with half lives of 10.6 (V1) and 5.2 (V2) hours. More than 60% of the applied urea were excreted with urine. Formed 14CO2 appeared at proportions of 66% (V2) and 71% (V1) in the respiration gases. Both, the decline of the 14C-activity in blood plasma and the specific 14C-activity of CO2 in the respiration gases after the end of the labelling period do not follow a kinetic of first order. The 15N-labelling of the NH3-N in ileal digesta was very high and reached plateau values similar with those of plasma urea (2.54 vs. 2.56 atom-% 15N-excess). A direct entry of plasma urea into the small intestine was concluded.
D000818:Animals; D004043:Dietary Fiber / Q000008:administration & dosage / Q000378:metabolism*; D007420:Intestine, Large / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D009584:Nitrogen / Q000378:metabolism*; D012756:Sheep / Q000378:metabolism*; D014508:Urea / Q000378:metabolism*
D004043:Dietary Fiber; D014508:Urea; D009584:Nitrogen
Untersuchungen zum Stickstoffumsatz im Dickdarm von Wiederkäuern. 4. Mitteilung. Umsatz und Isotopenbilanz von intrazäkal verabreichtem 14C- und 15N-Harnstoff bei Schafen bei gleichzeitiger intrazäkaler Gabe von teilhydrolysiertem Strohmehl.