1,25-Dihydroxyvitamin D3 receptors and their relationship to histological features in renal cell carcinoma.
The present studies were carried out in 11 human renal cell carcinomas to determine the presence of a receptor specific for an active form of vitamin D, 1,25-dihydroxyvitamin D3. Saturation and Scatchard analyses of the cytosol receptor for 1,25-dihydroxyvitamin D3 showed that nine tumors had a detectable level of the receptor (two fmol/mg. protein). The equilibrium dissociation constant of these receptors ranged between 46 and 380 pM and the binding capacity also ranged between 3.5 and 12.7 femtomol/mg. protein. Sucrose density gradient analysis of the specific binders revealed that the tumors had a receptor protein appearing as a single 3.6S peak. Two tumors which had only a trace of the receptor were high grade solid tumors consisting mainly of spindle or pleomorphic cells. Nine tumors possessing 1,25-dihydroxyvitamin D3 receptor consisted of clear and/or granular cells. Thus, the absence of the receptor was only accompanied by low differentiated sarcomatoid tumors with poor prognosis. However, so far, the amount of the receptor in the receptor-positive tumor did not relate to the other clinical and pathological features of the patients.
D000368:Aged; D002117:Calcitriol / Q000032:analysis*; D002292:Carcinoma, Renal Cell / Q000032:analysis* / Q000473:pathology; D005260:Female; D006801:Humans; D007668:Kidney / Q000473:pathology*; D007680:Kidney Neoplasms / Q000032:analysis* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D018167:Receptors, Calcitriol; D011987:Receptors, Steroid / Q000032:analysis*
D018167:Receptors, Calcitriol; D011987:Receptors, Steroid; D002117:Calcitriol