Cyclic 3',5'-adenosine monophosphate modulates vascular endothelial cell migration in vitro.
Using a modified Boyden chamber assay, we have examined the effect of cyclic nucleotides on bovine aortic endothelial cell migration in vitro. Dibutyrl cyclic 3',5'-adenosine monophosphate (5 mM) inhibited endothelial cell random migration by 67% and inhibited fibronectin-induced chemotaxis by 75%. Agents which significantly stimulated adenylate cyclase activity in endothelial cell membranes were also effective inhibitors of endothelial cell migration. Timolol blocked both the isoproterenol-induced stimulation of adenylate cyclase and the ability of isoproterenol to inhibit endothelial cell migration. Caffeine and isoproterenol together had a greater inhibitory effect on endothelial cell motility than either alone. These data suggest that cAMP may modulate vascular endothelial cell migration in an inhibitory fashion.
D000262:Adenylyl Cyclases / Q000378:metabolism; D000818:Animals; D001011:Aorta / Q000166:cytology / Q000187:drug effects; D003994:Bucladesine / Q000494:pharmacology*; D002110:Caffeine / Q000494:pharmacology; D002417:Cattle; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D002633:Chemotaxis / Q000187:drug effects; D000242:Cyclic AMP / Q000502:physiology*; D004727:Endothelium / Q000166:cytology* / Q000187:drug effects; D005333:Fetus; D005353:Fibronectins / Q000494:pharmacology; D007545:Isoproterenol / Q000494:pharmacology
D005353:Fibronectins; D002110:Caffeine; D003994:Bucladesine; D000242:Cyclic AMP; D000262:Adenylyl Cyclases; D007545:Isoproterenol