Chronic relapsing experimental allergic encephalomyelitis in the Lewis rat: studies on immune regulation.
To study immunoregulation of chronic relapsing experimental allergic encephalomyelitis (CR-EAE) in Lewis rats, we adoptively transferred concanavalin A-activated lymph node cells (LNC) or splenocytes, from hind footpad-inoculated donors at the onset (day 11), or recovery (day 16), of the first attack. Popliteal LNC, especially from day 16 donors, provided significant and dose-dependent, but incomplete, protection of recipients from encephalitogenic challenge; maximal mean delay in EAE onset was 10 days later than controls, with subsequent paralysis reduced more than 6-fold. In contrast, particularly from day 11 donors, superficial inguinal LNC recipients developed actively induced disease of normal severity up to 4 days earlier than CR-EAE controls. Furthermore day 11 EAE splenocytes, but not day 16 ones, adoptively transferred disease into 50-88% of naive recipients. In separate studies, we demonstrated unresponsiveness to active induction of disease in all rats re-challenged during stable late remission, as well as in a minority of animals pretreated with antigen in incomplete Freund's adjuvant. These results suggest an organ-dependent and time-dependent balance between effector and suppressor populations in the model.
D000818:Animals; D002908:Chronic Disease; D003584:Cytological Techniques; D004198:Disease Susceptibility; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology* / Q000473:pathology; D005260:Female; D005620:Freund's Adjuvant / Q000494:pharmacology; D006119:Groin; D006168:Guinea Pigs; D007109:Immunity; D007116:Immunization, Passive; D007717:Knee; D008198:Lymph Nodes / Q000473:pathology / Q000637:transplantation; D051381:Rats; D011917:Rats, Inbred Lew; D012008:Recurrence; D013116:Spinal Cord / Q000637:transplantation; D013154:Spleen / Q000473:pathology / Q000637:transplantation; D013950:Thymus Gland / Q000473:pathology
D005620:Freund's Adjuvant