title: BliI, a restriction endonuclease from Bacillus licheniformis.
abstract: From Bacillus licheniformis a site-specific restriction endonuclease, named BliI, has been purified and characterized. BliI was able to digest lambda DNA at pH 9.1 over a wide temperature range (25-65 degrees C). Digestion of lambda and psi X174 DNAs with BliI produced banding patterns identical to those seen with HaeIII. Therefore, BliI and HaeIII endonculeases are isoschizomers.
mesh_terms: D001407:Bacillus / Q000201:enzymology*; D004262:DNA Restriction Enzymes / Q000302:isolation & purification*; D004279:DNA, Viral; D015252:Deoxyribonucleases, Type II Site-Specific; D013379:Substrate Specificity
chemical_list: D004279:DNA, Viral; D004262:DNA Restriction Enzymes; D015252:Deoxyribonucleases, Type II Site-Specific; C024403:GGCC-specific type II deoxyribonucleases