The cytoskeletal protein vinculin is acylated by myristic acid.
In non-muscle cells the mechanism by which microfilament bundles interact with the plasma membrane is unclear. Vinculin, a 130 kDa protein found in adhesion plaques, has been postulated to have a role as a membrane anchor for microfilaments and we have investigated the biochemistry of this molecule in more detail. We report that a fraction of vinculin in chick embryo fibroblasts is acylated by myristic acid. This modification was present in both membrane-bound, cytoskeletal and cytosolic vinculin and thus did not determine preferential subcellular localisation. Myristic acid was also present in vinculin from cells transformed by Rous sarcoma virus.
D000215:Acylation; D000818:Animals; D001358:Avian Sarcoma Viruses; D002472:Cell Transformation, Viral; D002642:Chick Embryo; D003599:Cytoskeleton / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005347:Fibroblasts / Q000378:metabolism*; D007163:Immunosorbent Techniques; D008715:Methionine / Q000378:metabolism; D009124:Muscle Proteins / Q000378:metabolism*; D019814:Myristic Acid; D009227:Myristic Acids / Q000378:metabolism*; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000378:metabolism; D016596:Vinculin
D009124:Muscle Proteins; D009227:Myristic Acids; D010169:Palmitic Acids; D019814:Myristic Acid; D016596:Vinculin; D019308:Palmitic Acid; D008715:Methionine