Identification and definition of nucleolus-related fibrillar bodies in micronucleated cells.
Small nucleolus-related bodies which occur in the nucleoplasm of "micronuclei" lacking nucleolar organizers have been studied by immunofluorescence microscopy. These bodies stained specifically with three different antibodies directed against proteins that are normally associated with the dense fibrillar component of functional nucleoli, but not with antibodies specific for certain proteins of the granular component or the fibrillar centers. Our data show that, in the absence of rRNA genes, the various constituent proteins characteristic of the dense fibrillar component spontaneously assemble into spherical entities but that the subsequent fusion of these bodies into larger structures is prevented in these micronuclei. The similarity between these nucleolus-related bodies of micronuclei and the prenucleolar bodies characteristic of early stages of nucleologenesis during mitotic telophase is discussed.
D000818:Animals; D002460:Cell Line; D002466:Cell Nucleolus / Q000648:ultrastructure*; D005455:Fluorescent Antibody Technique; D007150:Immunohistochemistry; D008858:Microscopy, Phase-Contrast