Photoaffinity labeling of a novel benzodiazepine binding protein in rat brain.
Photoaffinity labeling experiments were conducted with [3H]clonazepam to investigate the possible existence of a benzodiazepine binding site that is distinct from the central- and peripheral-type benzodiazepine binding sites. These studies demonstrate the presence of a novel benzodiazepine binding protein in rat brain with a relative mobility of 65,000 daltons that binds benzodiazepines stereoselectivity in the high nanomolar-low micromolar concentration range.
D000345:Affinity Labels; D000818:Animals; D001345:Autoradiography; D001667:Binding, Competitive; D001923:Brain Chemistry; D002352:Carrier Proteins / Q000378:metabolism; D002998:Clonazepam / Q000378:metabolism; D005260:Female; D066298:In Vitro Techniques; D008970:Molecular Weight; D010777:Photochemistry; D051381:Rats; D011919:Rats, Inbred Strains; D011963:Receptors, GABA-A / Q000378:metabolism*
D000345:Affinity Labels; D002352:Carrier Proteins; D011963:Receptors, GABA-A; D002998:Clonazepam