The cytotoxic response to murine cytomegalovirus: requirements for the generation of MCMV-specific target cells.
The preparation of target cells susceptible to lysis by murine cytomegalovirus (MCMV)-immune T cells in vitro was investigated and found to be dependent upon target cell type, culture conditions, virus adsorption protocol and virus preparation. Optimally sensitive MCMV-infected targets were obtained by preculture of mouse embryo fibroblasts (MEF) in 3% vol/vol concanavalin-A-stimulated spleen cell supernatant (CSS)-supplemented medium, adsorption of salivary gland stock MCMV under 800 g centrifugation and at least 4 h further incubation at 37 degrees before addition of T cells. In contrast, salivary gland stock MCMV did not cause thioglycollate-induced peritoneal exudate cells to be susceptible to MCMV-specific T cell-mediated lysis, whereas tissue culture-passaged stock MCMV was successful. The preparation of MCMV-infected target cells is discussed in terms of the need for H-2 and viral antigen expression for T cell recognition.
D000818:Animals; D002478:Cells, Cultured; D002860:Chromium Radioisotopes; D003587:Cytomegalovirus / Q000276:immunology*; D003602:Cytotoxicity, Immunologic; D005347:Fibroblasts / Q000276:immunology; D007377:Interleukin-3 / Q000378:metabolism; D007700:Kinetics; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism
D002860:Chromium Radioisotopes; D007377:Interleukin-3