title: A demonstration that O2- is a crucial intermediate in the high quantum yield luminescence of luminol.
abstract: The chemiluminescence of luminol, due to its reaction with alkaline H2O2, is inhibited by superoxide dismutase or by hydroxyl radical scavengers. Hematin markedly enhances this H2O2-induced luminescence of luminol and lessens, but does not eliminate, the sensitivity towards these inhibitors. Reaction mechanisms are proposed to account for these results. Since luminol luminescence depends upon a reaction between the luminol radical and O2-, and since the luminol radical can reduce dioxygen to O2-, superoxide dismutase-inhibitable luminol luminescence cannot be reliably used as a detector of O2- production.
mesh_terms: D006427:Hemin; D006861:Hydrogen Peroxide; D006997:Hypochlorous Acid; D008163:Luminescent Measurements; D008165:Luminol; D011724:Pyridazines; D013482:Superoxide Dismutase / Q000378:metabolism; D013481:Superoxides
chemical_list: D011724:Pyridazines; D013481:Superoxides; D008165:Luminol; D006997:Hypochlorous Acid; D006427:Hemin; D006861:Hydrogen Peroxide; D013482:Superoxide Dismutase