Identification of the promoter of the Bacillus subtilis sdh operon.
The Bacillus subtilis sdhCAB operon contains the structural genes for the three subunits of the membrane bound succinate dehydrogenase complex. An sdh-specific transcript of about 3,450 nucleotides was detected in vegetative bacteria. S1 nuclease mapping experiments showed that the sdh operon is transcribed from a sigma-43 promoter; the transcript starts at a guanosine residue 90 base pairs upstream from the first gene of the operon, sdhC. No sdh transcript was found in B. subtilis carrying the sdh-115 mutation, which decreases expression of the sdh operon by more than 99%. The sdh-115 mutation is a G-to-A transition in the -35 region of the sigma-43 promoter. The sdh operon is sensitive to glucose repression. When the sdh promoter region was used to drive transcription of the cat-86 gene this gene also became glucose repressed.
D001412:Bacillus subtilis / Q000235:genetics*; D002874:Chromosome Mapping; D004262:DNA Restriction Enzymes; D005786:Gene Expression Regulation; D005798:Genes, Bacterial; D005947:Glucose / Q000502:physiology; D009876:Operon; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics; D012808:Sigma Factor / Q000235:genetics; D013385:Succinate Dehydrogenase / Q000235:genetics*; D014158:Transcription, Genetic
D012333:RNA, Messenger; D012808:Sigma Factor; D013385:Succinate Dehydrogenase; D004262:DNA Restriction Enzymes; D005947:Glucose