Testing for intron function in the essential Saccharomyces cerevisiae tRNA(SerUCG) gene.
The gene sup61+, which codes for the essential Saccharomyces cerevisiae tRNA(SerUCG), is the only single-copy tRNA gene in this organism know to contain an intron. To assess the role of this intron in tRNA gene expression, an intron-deleted sup61+ gene was constructed in vitro and introduced into the yeast genome. Isogenic intron- and intron+ strains were found to be indistinguishable by criteria that include growth rates, ability to undergo meiosis, levels of mature tRNA(SerUCG) transcribed in vivo, and the suppressor efficiency of amber- and ochre-specific alleles of this gene.
D005800:Genes, Fungal; D007438:Introns; D009693:Nucleic Acid Hybridization; D012331:RNA, Fungal / Q000235:genetics*; D012345:RNA, Transfer, Amino Acid-Specific / Q000235:genetics*; D012362:RNA, Transfer, Ser / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics; D013489:Suppression, Genetic; D014158:Transcription, Genetic
D012331:RNA, Fungal; D012345:RNA, Transfer, Amino Acid-Specific; D012362:RNA, Transfer, Ser