title: HLA antigens in colorectal tumours--low expression of HLA class I antigens in mucinous colorectal carcinomas.
abstract: Expression of HLA antigens and beta 2-microglobulin was studied by immunoperoxidase staining of frozen sections of 9 mucinous and 10 nonmucinous colorectal adenocarcinomas, 1 cloacogenic carcinoma, 12 colorectal adenomas and 4 samples of normal colorectal mucosa using monoclonal antibodies (MAbs). Staining results were related to histopathological features. HLA Class I antigens were strongly expressed in morphologically normal colorectal epithelium, in all adenomas tested and in all non-mucinous carcinomas. In contrast, expression of HLA class I antigens by the majority of tumour cells was present in only 2 of the 9 mucinous carcinomas, whereas 2 of these mucinous carcinomas were completely negative. In the mucinous carcinomas a striking scarcity of mononuclear inflammatory infiltrate, especially around the mucus accumulations, was observed. HLA class II antigen expression was not detected in normal epithelium and was only focally present in 1 of the 12 adenomas. In 6 out of the 20 carcinomas tested between 20% and 90% of the tumour cells were stained by MAbs against HLA class II antigens. Apart from the low expression of HLA class I antigens in mucinous carcinomas no relationship was found between expression of HLA antigens and histological features of the tumours. The relative poor prognosis of mucinous colorectal carcinoma as reported in the literature may be associated with low expression of HLA class I antigens and scant mononuclear inflammatory infiltrate, which may be a reflection of a weak immune response to the tumour cells.
mesh_terms: D000230:Adenocarcinoma / Q000276:immunology; D002288:Adenocarcinoma, Mucinous / Q000276:immunology*; D000951:Antigens, Neoplasm / Q000032:analysis*; D003110:Colonic Neoplasms / Q000276:immunology*; D006680:HLA Antigens / Q000032:analysis*; D006681:HLA-D Antigens / Q000032:analysis*; D006801:Humans; D007413:Intestinal Mucosa / Q000276:immunology; D012004:Rectal Neoplasms / Q000276:immunology*; D001613:beta 2-Microglobulin / Q000032:analysis
chemical_list: D000951:Antigens, Neoplasm; D006680:HLA Antigens; D006681:HLA-D Antigens; D001613:beta 2-Microglobulin