Biochemical characterization of mutants in cyclic nucleotide metabolism showing rhythmic conidiation in Neurospora.
Mutants, cpd-1, cpd-2, and bd, which have reduced concentrations of orthophosphate-repressible cyclic phosphodiesterase (cPDase), show reduced concentrations of cyclic 3',5'-AMP and exhibit rhythmic oscillations of cyclic 3',5'-AMP with about 24-hr period length. These mutants have either reduced concentrations of adenylate cyclase or enhanced concentrations of Mg-stimulated cyclic phosphodiesterase. These mutations changed the ability to activate heat-activated cyclic phosphodiesterase. White light reduced the concentration of cyclic 3',5'-GMP in mycelia.
D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases / Q000378:metabolism; D000262:Adenylyl Cyclases / Q000378:metabolism; D000242:Cyclic AMP / Q000032:analysis*; D006152:Cyclic GMP / Q000032:analysis*; D008274:Magnesium / Q000494:pharmacology; D009154:Mutation; D009491:Neurospora / Q000032:analysis / Q000235:genetics / Q000502:physiology*
D000242:Cyclic AMP; D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases; D000262:Adenylyl Cyclases; D006152:Cyclic GMP; D008274:Magnesium