Monoclonal antibodies against the major internal protein, p27, of a psoriasis-associated retrovirus-like particle.
Four murine monoclonal antibodies (MoAb) were raised against p27 isolated from psoriatic scale. The MoAb recognized the antigen on a subfraction (0.1-1%) of psoriatic lymphocytes as well as in skin biopsies from psoriatic patients. In contrast, the antigen could not be detected on lymphocytes or in skin biopsies from healthy controls. A sandwich ELISA assay using the MoAb for detection of p27 is described. This method, together with a competition antibody-binding assay for distinction of epitopes, demonstrates that the MoAb recognize four different antigenic determinants on p27.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002459:Cell Fusion; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006801:Humans; D008214:Lymphocytes / Q000276:immunology; D011565:Psoriasis / Q000097:blood / Q000276:immunology / Q000382:microbiology*; D011817:Rabbits / Q000276:immunology; D012191:Retroviridae Proteins / Q000032:analysis / Q000276:immunology*
D000911:Antibodies, Monoclonal; D012191:Retroviridae Proteins