A point mutation at codon 13 of the N-ras oncogene in a human stomach cancer.
A surgically removed human stomach cancer with the histological diagnosis of poorly differentiated adenocarcinoma contained an activated N-ras oncogene detected by an in vivo selection assay in nude mice using transfected NIH3T3 cells. Analysis using synthetic 20-mer oligonucleotide probes revealed a point mutation from G to C at the first letter of codon 13 of the N-ras gene resulting in the substitution of arginine for glycine. This is the first observation of an activated N-ras oncogene in human stomach cancers.
D000230:Adenocarcinoma / Q000235:genetics; D002882:Chromosomes, Human, Pair 13; D003062:Codon; D003562:Cytidine; D004262:DNA Restriction Enzymes / Q000378:metabolism; D006147:Guanine; D006801:Humans; D009154:Mutation; D009857:Oncogenes; D012333:RNA, Messenger; D013274:Stomach Neoplasms / Q000235:genetics*
D003062:Codon; D012333:RNA, Messenger; D003562:Cytidine; D006147:Guanine; D004262:DNA Restriction Enzymes