Deoxycytidine, insulin and epidermal growth factor overcome the effect of two natural inhibitors of the haemopoietic system.
The effect of 2'-deoxycytidine on a pluripotent haemopoietic stem cell proliferation inhibitor, as assayed in the mouse spleen colony technique and on the synthetic haemoregulatory pentapeptide (acting predominantly on myeloid determined cells) as assayed in the agar colony technique, was studied. 2'-deoxycytidine was able to overcome the effect of both inhibitors. The inhibition was also blocked by insulin or epidermal growth factor. Since these results are in agreement with those on other cells and other inhibitors, they may describe a more general phenomenon. It is suggested that the mode of action of the inhibitors involves, probably indirectly, interaction with the activity of ribonucleotide reductase.
D000818:Animals; D002455:Cell Division / Q000187:drug effects*; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D003841:Deoxycytidine / Q000494:pharmacology*; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D006410:Hematopoiesis / Q000187:drug effects*; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000187:drug effects*; D007328:Insulin / Q000494:pharmacology*; D008222:Lymphokines / Q000037:antagonists & inhibitors*; D051379:Mice; D008815:Mice, Inbred Strains; D009842:Oligopeptides / Q000494:pharmacology
D007328:Insulin; D008222:Lymphokines; D009842:Oligopeptides; C022173:stem cell inhibitory factor; D003841:Deoxycytidine; D004815:Epidermal Growth Factor