Release of leukotrienes during rapid expulsion of Trichinella spiralis from immune rats.
Rapid expulsion of the nematode Trichinella spiralis from immune rats is associated with an increase in volume of intestinal exudate and the presence of large numbers of tissue mucosal mast cells (MMC) and eosinophils. We have measured the concentrations of leukotrienes (LT) C4 (LTC4) and B4 (LTB4) in gut perfusates and mucosal homogenates at 30 min, 1, 3, 6 and 20 hr after challenge with larvae. Leukotrienes were identified by radioimmunoassay (RIA) combined with reverse-phase high-pressure liquid chromatography (RP-HPLC). There were significant elevations at 30 min and 1 hr in the concentrations of LTC4 in the perfusates from the gut of challenged immune rats compared to controls (infected unchallenged and uninfected naive rats). Similar increases in immunoreactive LTC4 and LTB4 were observed in mucosal homogenates from the gut of immune challenged animals. A second peak of LTB4 was also observed at 20 hr in both immune and naive challenged rats. There were also elevations in serum concentration of the MMC-associated specific serine protease, rat mast cell protease II (RMCPII). Since LTC4 causes smooth muscle contraction, increased vascular permeability and stimulation of mucus hypersecretion, and LTB4 recruits and activates inflammatory cells, leukotrienes may participate in the process of rapid expulsion of T. spiralis.
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D053818:Chymases; D010450:Endopeptidases / Q000097:blood; D005260:Female; D007109:Immunity; D007421:Intestine, Small / Q000276:immunology; D007975:Leukotriene B4 / Q000378:metabolism*; D008407:Mast Cells / Q000276:immunology; D011863:Radioimmunoassay; D051381:Rats; D013189:SRS-A / Q000378:metabolism*; D012697:Serine Endopeptidases; D014235:Trichinellosis / Q000276:immunology* / Q000469:parasitology
D013189:SRS-A; D007975:Leukotriene B4; D010450:Endopeptidases; D012697:Serine Endopeptidases; D053818:Chymases