Pre-mRNA splicing and the nuclear matrix.
We examined the relationship between pre-mRNA splicing and the nuclear matrix by using an in vivo system that we have developed. Plasmids containing the inducible herpesvirus tk gene promoter linked to an intron-containing segment of the rabbit beta-globin gene were transfected into HeLa cells, and then the promoter was transactivated by infection with a TK- virus. Northern analysis revealed that the globin pre-mRNA and all its splicing intermediates and products are associated with the nuclear matrix prepared from such transfected cells. When the nuclear matrix was incubated with a HeLa cell in vitro splicing extract in the presence of ATP, the amount of matrix-associated precursor progressively decreased without a temporal lag in the reaction, with a corresponding increase in free intron lariat. Thus, most of the events of the splicing process (endonucleolytic cuts and branching) occur in this in vitro complementation reaction. However, ligation of exons cannot be monitored in this system because of the abundance of preexisting mature mRNA. Since the matrix is not a self-splicing entity, whereas the in vitro splicing system cannot process efficiently deproteinized matrix RNA, we conclude from our in vitro complementation results (which can be reproduced by using micrococcal nuclease-treated splicing extract) that the nuclear matrix preparation retains parts of preassembled ribonucleoprotein complexes that have the potential to function when supplemented with soluble factors (presumably other than most of the small nuclear ribonucleoproteins known to participate in splicing) present in the HeLa cell extract.
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism*; D004262:DNA Restriction Enzymes; D005796:Genes; D005814:Genes, Viral; D005914:Globins / Q000235:genetics; D006367:HeLa Cells / Q000378:metabolism; D006801:Humans; D009694:Nucleic Acid Precursors / Q000235:genetics*; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012322:RNA Precursors; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics*; D011817:Rabbits; D018139:Simplexvirus / Q000235:genetics; D013937:Thymidine Kinase / Q000235:genetics; D014709:Vero Cells
D009694:Nucleic Acid Precursors; D012322:RNA Precursors; D012333:RNA, Messenger; D005914:Globins; D013937:Thymidine Kinase; D004262:DNA Restriction Enzymes