Interrelationship between hemolysis and lipid peroxidation of human erythrocytes induced by silicic acid and silicate dusts.
Silicic acid and silicate dusts (slate dust and chrysotile asbestos) cause hemolysis of erythrocytes in vitro. The peroxidation of polyunsaturated fatty acids (PUFA) of erythrocyte membrane lipids is also enhanced by incubating the erythrocytes with silicic acid and silicate dusts in in vitro. Hemolysis of erythrocytes elicited by silicic acid and silicate dusts is inhibited significantly by polyvinyl-pyrrolidone and dipalmitoyl lecithin (DPL). These agents, however, have no effect on silicic acid and silicate dust induced peroxidation of erythrocyte membrane lipids. On the other hand, peroxidation of erythrocyte membrane lipids, induced by silicic acid and silicate dusts, is inhibited almost completely by adding superoxide dismutase and catalase to the incubation system, whilst the hemolysis of erythrocytes induced by silicic acid and silicate dusts is unaffected by these agents. Similarly the lysis of erythrocytes, induced by silicic acid and silicate dusts, proceeds at a much faster rate than silicic acid and silicate dust induced lipid peroxidation. These results indicate that silicic acid and silicate dust induced hemolysis and lipid peroxidation represent two independent processes.
D001194:Asbestos / Q000633:toxicity; D017632:Asbestos, Serpentine; D004391:Dust / Q000009:adverse effects*; D004910:Erythrocyte Membrane / Q000378:metabolism; D004912:Erythrocytes / Q000378:metabolism*; D005231:Fatty Acids, Unsaturated / Q000097:blood; D006461:Hemolysis / Q000187:drug effects*; D006801:Humans; D066298:In Vitro Techniques; D008054:Lipid Peroxides / Q000097:blood*; D012824:Silicic Acid / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D012822:Silicon Dioxide / Q000494:pharmacology*
D017632:Asbestos, Serpentine; D004391:Dust; D005231:Fatty Acids, Unsaturated; D008054:Lipid Peroxides; D001194:Asbestos; D012824:Silicic Acid; D012822:Silicon Dioxide