An amplification unit in human melanoma cells showing partial homology with sequences of human papillomavirus type 9 and with nuclear antigen 1 of the Epstein-Barr virus.
By partial homology with the DNA of human papillomavirus type 9 a cellular amplification unit was detected which is amplified in melanoma cells but not in Epstein-Barr virus-transformed B cells of two melanoma patients. A 2.4-kilobase EcoRI fragment of this amplification unit was cloned and designated mel/HPV9. At the chromosomal level we detected mel/HPV9 in homogeneously staining regions or in abnormally banded regions containing different marker chromosomes of both melanoma cell lines. DNA sequence analysis of a part of mel/HPV 9 revealed homology with the third internal repeat array of Epstein-Barr virus nuclear antigen 1.
D000954:Antigens, Surface / Q000032:analysis; D000956:Antigens, Viral / Q000235:genetics*; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000032:analysis*; D019309:Epstein-Barr Virus Nuclear Antigens; D005784:Gene Amplification; D006801:Humans; D008545:Melanoma / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D009857:Oncogenes; D027383:Papillomaviridae / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
D000954:Antigens, Surface; D000956:Antigens, Viral; D004279:DNA, Viral; D019309:Epstein-Barr Virus Nuclear Antigens