Identification of a receptor for peptides of the bombesin family in Swiss 3T3 cells by affinity cross-linking.
The cross-linking agent ethylene glycol-bis(succinimidyl succinate) was used to covalently link 125I-labeled gastrin releasing peptide (125I-GRP) to an Mr 75,000-85,000 surface protein in Swiss 3T3 cells that displays many characteristics of a specific receptor for peptides of the bombesin family. This protein was not present in other cell lines which do not exhibit receptors for bombesin-like peptides. Unlabeled GRP competed for affinity labeling of the Mr 75,000-85,000 protein in a concentration-dependent manner, and other bombesin-related peptides also inhibited the cross-linking of 125I-GRP to this component. In contrast, high concentrations of a variety of other peptide hormones and mitogens had no effect. Affinity labeling of the Mr 75,000-85,000 protein was dependent on the concentration of 125I-GRP and exhibited saturability. 125I-GRP affinity labeling of this protein was also demonstrated by two-dimensional gel electrophoresis. These studies suggest that an Mr 75,000-85,000 surface protein with an isoelectric point of 6.0 to 6.5 is a major component of the receptor for peptides of the bombesin family in Swiss 3T3 cells.
D000345:Affinity Labels; D000818:Animals; D001667:Binding, Competitive; D002460:Cell Line; D003432:Cross-Linking Reagents; D004591:Electrophoresis, Polyacrylamide Gel; D019886:Gastrin-Releasing Peptide; D007526:Isoelectric Point; D051379:Mice; D008970:Molecular Weight; D010455:Peptides / Q000378:metabolism; D018004:Receptors, Bombesin; D017981:Receptors, Neurotransmitter / Q000032:analysis* / Q000378:metabolism; D013388:Succinimides
D000345:Affinity Labels; D003432:Cross-Linking Reagents; D010455:Peptides; D018004:Receptors, Bombesin; D017981:Receptors, Neurotransmitter; D013388:Succinimides; C028722:ethylene glycolylbis(succinimidyl succinate); D019886:Gastrin-Releasing Peptide