Properties of a U1 RNA enhancer-like sequence.
The properties of a X.laevis U1B snRNA gene enhancer have been studied by microinjection in Xenopus oocytes. The enhancer-like sequence, defined as a short DNA stretch that is able to activate transcription in an orientation independent manner, is interchangeable between different U snRNA genes. The enhancer sequence alone does not, however, efficiently activate transcription from an SV40 pol II promoter but regains its activity when combined with the U-gene specific proximal sequence element. DNase I protection experiments show that the X.laevis U1B enhancer can interact specifically with a nuclear factor present in mammalian cells.
D000818:Animals; D001483:Base Sequence; D004262:DNA Restriction Enzymes; D004742:Enhancer Elements, Genetic; D005260:Female; D005796:Genes; D005809:Genes, Regulator; D009865:Oocytes / Q000378:metabolism; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012342:RNA, Small Nuclear / Q000235:genetics*; D013539:Simian virus 40 / Q000235:genetics; D014158:Transcription, Genetic; D014982:Xenopus laevis
D012342:RNA, Small Nuclear; D004262:DNA Restriction Enzymes