Bovine viral diarrhea virus-infected MDBK monolayer as antigen in enzyme-linked immunosorbent assay (ELISA) for the measurement of antibodies in bovine sera.
A specific ELISA for the detection of IgG antibodies against bovine viral diarrhea virus (BVDV) has been developed and was compared with the seroneutralization (SN) titers for a total of 60 bovine serum samples. A BVDV-infected Madin-Darby Bovine Kidney (MDBK) cell monolayer served as test antigen. The following results were obtained: a coefficient of correlation (r) of 0.63 (P less than 0.01) between BVDV-ELISA and SN titer in BVDV-seroconverted animals and 0.71 for all sera, including that from BVDV vaccinated animals; a sensitivity of 92%, a specificity of 91%, a concordance of 92%; and a demonstration of a parallel increase in BVDV-ELISA and SN titers in animals with seroconversion. The BVDV-ELISA permits a more complete evaluation of the humoral immune response to BVDV.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D000956:Antigens, Viral / Q000276:immunology*; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000276:immunology*; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology*; D002460:Cell Line; D001908:Diarrhea Viruses, Bovine Viral / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D007074:Immunoglobulin G / Q000032:analysis*; D009500:Neutralization Tests; D010576:Pestivirus / Q000276:immunology*
D000914:Antibodies, Viral; D000956:Antigens, Viral; D007074:Immunoglobulin G