Purification of alpha 2,6-sialyltransferase from rat liver by dye chromatography.
We describe a simple three-step purification for Gal-beta 1,4-GlcNAc-alpha 2,6-sialyltransferase (EC 2.4.99.1) from rat liver which uses chromatography on Cibacron Blue F3GA and f.p.l.c. It gives a highly purified (11,000-fold) enzyme in 19% yield, which is free of other sialyltransferases, CMP-NeuAc hydrolase, sialidases and proteinases.
D000818:Animals; D002846:Chromatography, Affinity / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D004396:Coloring Agents; D004591:Electrophoresis, Polyacrylamide Gel; D008099:Liver / Q000201:enzymology*; D051381:Rats; D012685:Sepharose / Q000031:analogs & derivatives; D012799:Sialyltransferases / Q000302:isolation & purification*; D000094802:beta-D-Galactoside alpha 2-6-Sialyltransferase
D004396:Coloring Agents; C017842:cibacron blue-sepharose; D012685:Sepharose; D012799:Sialyltransferases; D000094802:beta-D-Galactoside alpha 2-6-Sialyltransferase