A new lectin-gold complex for ultrastructural localization of galacturonic acids.
We report the development of a cytochemical affinity technique for detection of galacturonic acids at the ultrastructural level. The highly purified gonad lectin from Aplysia depilans (AGL) was tagged with colloidal gold particles and used for labeling carbohydrates in resin-embedded sections of various plant and fungal tissues. Patterns of AGL binding sites were compared to those obtained with a D-galactose-specific lectin, Ricinus communis agglutinin I. Differences in labeling patterns were noted, indicating that the lectins exhibited differential carbohydrate binding. In addition, the considerable loss of labeling over isolated wheat coleoptile walls treated for removal of pectin, after incubation with the AGL-gold complex, strongly suggested an affinity of AGL for pectic substances. A series of cytochemical controls, including sugar inhibition tests, has proven the specificity of the technique and the high affinity of AGL towards galacturonic acids. The potential value of this new lectin for ultrastructural studies on cell wall pectic substances in plant biology and pathology is demonstrated.
D002176:Candida albicans / Q000032:analysis; D002367:Ricinus communis; D002473:Cell Wall / Q000032:analysis; D002614:Chelating Agents; D003102:Colloids; D004121:Dimethyl Sulfoxide; D005063:Europium; D005456:Fluorescent Dyes; D005658:Fungi / Q000032:analysis*; D037161:Galectins; D006046:Gold; D006388:Hemagglutinins; D006603:Hexuronic Acids / Q000032:analysis*; D037102:Lectins; D008854:Microscopy, Electron; D037121:Plant Lectins; D010944:Plants / Q000032:analysis*; D010947:Plants, Toxic; D014574:Uronic Acids / Q000032:analysis*
D002614:Chelating Agents; D003102:Colloids; D005456:Fluorescent Dyes; D037161:Galectins; D006388:Hemagglutinins; D006603:Hexuronic Acids; D037102:Lectins; D037121:Plant Lectins; C017707:Ricinus communis agglutinin-1; D014574:Uronic Acids; D005063:Europium; C007819:galacturonic acid; D006046:Gold; D004121:Dimethyl Sulfoxide