High affinity binding of reovirus type 3 to cells that lack beta adrenergic receptor activity.
A previous report demonstrated both immunological crossreactivity and structural similarity between the mammalian beta adrenergic receptor and the cell surface receptor for the reovirus type 3 (14). We now demonstrate that reovirus type 3 can bind selectively and with high affinity to cells that lack beta adrenergic receptor activity (L-cells). The present study was also designed to determine what effect reovirus binding has on beta adrenergic receptor function in cells (DDT1) that possess an intact ligand binding site. Based on computer analysis of reovirus competitive inhibition curves, the apparent dissociation binding constants (Kd) for reovirus binding to DDT1 and L-cells are 0.1 nM and 0.25 nM, respectively. High affinity [125I]-iodocyanopindolol (CYP) binding to beta adrenergic receptors can also be demonstrated in DDT1 cells but not in L-cells. In agreement with these ligand binding studies, adenylate cyclase activity is stimulated by the beta receptor agonist isoproterenol in DDT1 cell membranes but not in L-cell membranes. In addition, isoproterenol increases cAMP levels in DDT1 cells but not in L-cells. Neither reovirus serotype stimulates cAMP levels in either cell line, nor do they influence beta-adrenergic agonist stimulation of cAMP in DDT1 cells. These results argue against identity of the receptors for reovirus type 3 and beta adrenergic ligands.
D000262:Adenylyl Cyclases / Q000378:metabolism; D000526:Alprenolol / Q000494:pharmacology; D000818:Animals; D001667:Binding, Competitive; D002460:Cell Line; D020120:Iodocyanopindolol; D007545:Isoproterenol / Q000494:pharmacology; D012089:Mammalian orthoreovirus 3 / Q000378:metabolism*; D010869:Pindolol / Q000031:analogs & derivatives / Q000494:pharmacology; D011869:Radioligand Assay; D011943:Receptors, Adrenergic, beta / Q000187:drug effects / Q000378:metabolism*; D011991:Receptors, Virus / Q000378:metabolism*; D012087:Reoviridae / Q000378:metabolism*
D011943:Receptors, Adrenergic, beta; D011991:Receptors, Virus; C046176:reovirus type 3 receptor; D020120:Iodocyanopindolol; D000526:Alprenolol; D010869:Pindolol; D000262:Adenylyl Cyclases; D007545:Isoproterenol