Frontal gel chromatography of interacting systems: theoretical and experimental evaluation of the shapes of elution profiles for systems of the type A + B in equilibrium C.
A theoretical and experimental study has been made of the advancing elution profile in frontal gel chromatography of interacting systems for which the elution volume of the complex is smaller than that of the larger reactant. First, Gilbert-Jenkins theory is used to delineate the form of the elution profile from the magnitudes of the elution volumes and concentrations of reacting species. This procedure resulted in the detection of a misinterpretation of certain patterns obtained in a gel chromatographic study of the interaction between myoglobin and ovalbumin. Second, a numerical computational procedure, which incorporates both axial dispersion and concentration-dependence of species elution volumes, is used to establish the influence of these two factors on boundary shapes for such systems. Third, frontal gel chromatography on Sephadex G-75 is used to compare experimental behavior with theoretical profiles predicted for the electrostatic interaction between cytochrome c and soybean trypsin inhibitor (pH 6.8, I 0.01). Results of these experiments serve as a guide for future conduct of experiments aimed at characterization of biologically important, reversible complex formation between proteins and/or other macromolecules.
D002850:Chromatography, Gel / Q000379:methods*; D003574:Cytochrome c Group / Q000032:analysis; D008433:Mathematics; D008956:Models, Chemical; D009211:Myoglobin / Q000032:analysis; D010047:Ovalbumin / Q000032:analysis; D014361:Trypsin Inhibitors / Q000032:analysis
D003574:Cytochrome c Group; D009211:Myoglobin; D014361:Trypsin Inhibitors; D010047:Ovalbumin