cDNA clone encoding a high molecular weight antigen of Babesia bovis.
An expression library was constructed by inserting cDNA copied from mRNA of the blood stages of Babesia bovis isolate KA into bacteriophage lambda gt11-amp3. An antigen-positive cDNA clone detected by screening the library with antibodies from cattle vaccinated with the KA isolate was shown to encode part of a high-molecular weight polypeptide antigen of B. bovis. This molecule was a dominant immunogen and was found by immunofluorescence to be within the parasite in infected erythrocytes.
D000818:Animals; D000953:Antigens, Protozoan / Q000032:analysis / Q000235:genetics*; D001403:Babesia / Q000235:genetics* / Q000276:immunology; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D004262:DNA Restriction Enzymes; D004251:DNA Transposable Elements; D015246:Deoxyribonuclease EcoRI; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000469:parasitology; D007118:Immunoassay; D009693:Nucleic Acid Hybridization; D010455:Peptides / Q000032:analysis / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics
D000953:Antigens, Protozoan; D004251:DNA Transposable Elements; D010455:Peptides; D012333:RNA, Messenger; D004247:DNA; D004262:DNA Restriction Enzymes; D015246:Deoxyribonuclease EcoRI