[The use of transposons for the mutagenesis of R. phaseoli and R. japonicum].
Plasmid pSUp2011 has been used to transfer transposon Tn5 into the cells of R. japonicum 110 and R. phaseoli 693. Transposition of Tn5 into the chromosomes of R. japonicum and R. phaseoli has been demonstrated,resulting in isolation of auxotrophic and symbiotic mutants of both strains. The frequencies of selected auxotrophic mutations have reached 4% in R. japonicum 110 and 0.6% in R. phaseoli 693. Streptomycin resistance gene locating on Tn5 has been found to be phenotypically expressed in R. japonicum 110 and R. phaseoli 693 cells.
D004251:DNA Transposable Elements; D004352:Drug Resistance, Microbial; D005819:Genetic Markers; D009154:Mutation; D012231:Rhizobium / Q000235:genetics*
D004251:DNA Transposable Elements; D005819:Genetic Markers
Ispol'zovanie transpozonov dlia mutageneza R. phaseoli i R. japonicum.