Complete sequence and structure of the gene for human adenosine deaminase.
The nucleotide sequence of the human adenosine deaminase gene was determined. The gene was isolated in a series of overlapping lambda phage clones containing human germ line DNA. A total of 36,741 base pairs were sequenced, including 32,040 base pairs from the transcription initiation site to the polyadenylation site, 3935 base pairs of 5'-flanking DNA, and 766 base pairs of 3'-flanking DNA. The gene contains 12 exons separated by 11 introns. The exons range in size from 62 to 325 base pairs while the introns are 76-15 166 base pairs in size. The area sequenced contains 23 copies of Alu repetitive DNA and a single copy of an "O" family repeat. All but one of these repeat sequences are located in the first three introns or the 5'-flanking region. The apparent promoter region of the gene lacks the "TATA" and "CAAT" sequences often found in eucaryotic promoters and is extremely G/C rich. Contained within this region are areas homologous to other G/C-rich promoters, including six decanucleotide sequences that are highly homologous to sequences identified as functional binding sites for transcription factor Sp1.
D000243:Adenosine Deaminase / Q000235:genetics*; D000595:Amino Acid Sequence; D010582:Bacteriophage lambda / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism; D004262:DNA Restriction Enzymes; D005091:Exons; D005260:Female; D005796:Genes; D006801:Humans; D007438:Introns; D009700:Nucleoside Deaminases / Q000235:genetics*; D010920:Placenta / Q000201:enzymology; D011247:Pregnancy; D014158:Transcription, Genetic
D004247:DNA; D004262:DNA Restriction Enzymes; D009700:Nucleoside Deaminases; D000243:Adenosine Deaminase