Isolated rectal gland cells: oxygen consumption and hormonal stimulation.
Cells isolated from rectal glands of Squalus acanthias, using collagenase and hyaluronidase digestion, retained normal morphological characteristics as judged by light microscopy of 1-micron plastic sections. Their oxygen consumption per unit weight was comparable to that of intact rectal gland studied either in situ, or by isolated perfusion, as well as that of rectal gland slices. Cellular respiration was stimulated by dibutyryl cyclic AMP and theophylline or by vasoactive intestinal peptide which stimulate secretion of chloride by the intact gland. Stimulated oxygen consumption was inhibited by ouabain and bumetanide and was proportional to the concentration of sodium or chloride in the incubation solution. The oxygen consumption of these cells parallels the secretory and metabolic behavior of the intact rectal gland, suggesting that it reflects energy demands for ion transport. The relative ease with which a homogeneous preparation of viable and active cells can be obtained and the apparent preservation of many of their key functional characteristics make this preparation a useful tool for the study of hormone-stimulated ion transport.
D000241:Adenosine / Q000494:pharmacology; D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D002712:Chlorides / Q000378:metabolism; D004284:Dogfish; D006728:Hormones / Q000494:pharmacology*; D008858:Microscopy, Phase-Contrast; D008985:Monensin / Q000494:pharmacology; D010042:Ouabain / Q000494:pharmacology; D010101:Oxygen Consumption / Q000187:drug effects; D012491:Salt Gland / Q000166:cytology*; D012754:Sharks; D013806:Theophylline / Q000494:pharmacology; D014660:Vasoactive Intestinal Peptide / Q000494:pharmacology
D002712:Chlorides; D006728:Hormones; D014660:Vasoactive Intestinal Peptide; D010042:Ouabain; D003994:Bucladesine; D008985:Monensin; D013806:Theophylline; D000241:Adenosine