[Cloning of genes for proline biosynthesis in Escherichia coli].
Restriction map of Escherichia coli chromosome fragment (7.4 MD) carrying proAB genes was constructed. Localization of proA and proB genes on the cloned chromosome fragment was determined by complementation test and the measuring of glutamylkinase activity (proB gene product). ProA and proB genes were cloned separately on multicopy plasmids of alternative orientation and their expression being, probably, under the control of their own regulatory regions, studied.
D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D005798:Genes, Bacterial; D010957:Plasmids; D011392:Proline / Q000096:biosynthesis* / Q000235:genetics
D011392:Proline; D004262:DNA Restriction Enzymes
Klonirovanie genov biosinteza prolina Escherichia coli.