Expression of the histidine-rich protein PfHRP1 by knob-positive Plasmodium falciparum is not sufficient for cytoadherence of infected erythrocytes.
Six culture-adapted knob-positive Plasmodium falciparum parasites, four of which were nonbinding in an in vitro cytoadherence assay, were tested for the presence of the knob-associated histidine-rich protein PfHRP1. Two knob-positive, strongly cytoadherence-positive P. falciparum strains from Aotus monkeys were also examined. All parasites expressed PfHRP1, suggesting that it plays a structural or functional role related to knobs but is not by itself sufficient for cytoadherence of P. falciparum-infected erythrocytes.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000953:Antigens, Protozoan / Q000502:physiology*; D015153:Blotting, Western; D002448:Cell Adhesion; D004910:Erythrocyte Membrane / Q000648:ultrastructure; D004912:Erythrocytes / Q000469:parasitology*; D005455:Fluorescent Antibody Technique; D006801:Humans; D008565:Membrane Proteins / Q000502:physiology; D010963:Plasmodium falciparum / Q000502:physiology*
D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan; D008565:Membrane Proteins