[Glutamate dehydrogenase--an activator of NAD-kinase in the rabbit liver].
An electrophoretically homogeneous preparation of a NAD-kinase activator from rabbit liver was obtained and its physico-chemical properties were investigated. The molecular mass of the monomer and oligomer, pI, number of SH-groups per enzyme subunit and some other factors were determined. The similarity of activator properties to those of glutamate dehydrogenase and the revealed glutamate dehydrogenase activity of the NAD-kinase activator permitted to identify the latter as glutamate dehydrogenase. It was demonstrated that the enzyme activates NAD-kinase 2-4 times already at the glutamate dehydrogenase: NAD-kinase ratio of 2:1. The effect of glutamate dehydrogenase on the enzyme consists in an increase of Vmax; the KmNAD value for the NAD-kinase reaction remains thereby unchanged. The physiological role of the interaction between the two enzymes is discussed.
D000818:Animals; D004789:Enzyme Activation; D005969:Glutamate Dehydrogenase / Q000302:isolation & purification* / Q000378:metabolism; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D010770:Phosphotransferases / Q000302:isolation & purification / Q000378:metabolism*; D017853:Phosphotransferases (Alcohol Group Acceptor); D011817:Rabbits; D014018:Tissue Distribution
D005969:Glutamate Dehydrogenase; D010770:Phosphotransferases; D017853:Phosphotransferases (Alcohol Group Acceptor); C021844:NAD kinase
Aktivator NAD-kinazy iz pecheni krolika--glutamatdegidrogenaza.