Suitability of oligopeptides for induction of hormonal imprinting--implications on receptor and hormone evolution.
Hormonal imprinting induced in Tetrahymena and in Chang liver cells with di-, tri-, tetra- and pentapeptides (synthetic opioids and their fragments) has shown that both cell types are able to differentiate the related molecules from one another. The dipeptide phenylalanine + proline induced a measurable imprinting in the liver cells, and chain length increase, especially terminal coupling with tyrosine enhanced the imprinting potential enormously. Intra-chain changes in the amino acid sequence had a measurable effect on the intensity of imprinting. The molecules showing the relatively strongest physiological action accounted for the most intensive imprinting in both cell types; this indicates that, in all probability, induction of binding site formation plays a key role in the development of signal molecules, and thereby in hormone evolution.
D000818:Animals; D002460:Cell Line; D006728:Hormones / Q000096:biosynthesis*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D009842:Oligopeptides / Q000494:pharmacology*; D011956:Receptors, Cell Surface / Q000096:biosynthesis*; D013768:Tetrahymena / Q000187:drug effects / Q000378:metabolism
D006728:Hormones; D009842:Oligopeptides; D011956:Receptors, Cell Surface