title: Neutrophil lactoferrin content: variation among mammals.
abstract: Lactoferrin (Lf) in blood and/or marrow neutrophils was semiquantified using indirect immunofluorescence technique in nine mammalian species. Neutrophil iron-binding reactivity (NFeBR), which corresponds primarily to Lf, was also visualized and semiquantified using functional cytochemical (FeNTA-AF) technique at the light microscopic level in these nine and in an additional fifteen mammalian species, and in selected species at the ultrastructural level. Neutrophil immunoreactive Lf was positively correlated with total cellular and granule content of NFeBR among these nine species, and with previously reported concentrations of neutrophil Lf quantified by radioimmunoassay. Relative levels of Lf in neutrophil extracts from rat, hamster, and human were confirmed using SDS-polyacrylamide gel electrophoresis and immunoblotting. Relatively high levels of immunoreactive neutrophil Lf and/or NFeBR were observed in carnivores (ten species) and primates (six species). Among rodents (five species), the levels were variable, and the artiodactyls (four species) studied had low levels. These results demonstrate that neutrophil Lf levels vary widely among mammalian species. In addition, FeNTA-AF technique provides a rapid means of evaluating animals for relative quantities of neutrophil Lf.
mesh_terms: D000818:Animals; D001188:Artiodactyla / Q000378:metabolism; D001853:Bone Marrow / Q000032:analysis; D002335:Carnivora / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D006801:Humans; D007118:Immunoassay; D007781:Lactoferrin / Q000032:analysis*; D007782:Lactoglobulins / Q000032:analysis*; D008322:Mammals / Q000378:metabolism*; D009504:Neutrophils / Q000032:analysis* / Q000648:ultrastructure; D011323:Primates / Q000378:metabolism; D012377:Rodentia / Q000378:metabolism; D013045:Species Specificity
chemical_list: D007782:Lactoglobulins; D007781:Lactoferrin