Periodate-oxidized NADP+ is a powerful inhibitor of human placental estradiol-17 beta dehydrogenase.
Periodate-oxidized NADP+ inhibits the NAD+-linked activity of human placental estradiol-17 beta dehydrogenase (EC 1.1.1.62). The inhibition appears to be competitive with respect to NAD+ and can be reversed by dialysis or gel filtration. The apparent inhibitor constant for the periodate-oxidized analogue is 0.047 microM. The presence in the incubation mixture of NAD+ protects the enzyme against inhibition. No inhibitory effects of the coenzyme analogue are observed on the NADP+-linked activity of the enzyme.
D015067:17-Hydroxysteroid Dehydrogenases / Q000037:antagonists & inhibitors*; D002850:Chromatography, Gel; D003956:Dialysis; D004958:Estradiol / Q000378:metabolism; D004960:Estradiol Dehydrogenases / Q000037:antagonists & inhibitors*; D006801:Humans; D007700:Kinetics; D009243:NAD / Q000378:metabolism; D009249:NADP / Q000378:metabolism*; D010504:Periodic Acid / Q000494:pharmacology*; D010920:Placenta / Q000201:enzymology*
D009243:NAD; D010504:Periodic Acid; D004958:Estradiol; D009249:NADP; D015067:17-Hydroxysteroid Dehydrogenases; D004960:Estradiol Dehydrogenases