Immunohistochemical localization of phosphatidylinositol-4,5-bisphosphate in the rat lens.
We have attempted to localize immunohistochemically phosphatidylinositol-4,5-bisphosphate (PIP2) in rat lens tissue using affinity purified rabbit anti-PIP2 antibodies. Evidence indicates that PIP2 is localized to the lens epithelial cells but appears to be absent from the lens fiber cells.
D000818:Animals; D000906:Antibodies; D006651:Histocytochemistry; D007124:Immunoenzyme Techniques; D007158:Immunologic Techniques; D007908:Lens, Crystalline / Q000201:enzymology*; D019269:Phosphatidylinositol 4,5-Diphosphate; D010716:Phosphatidylinositols / Q000032:analysis* / Q000276:immunology; D010744:Phosphoric Monoester Hydrolases; D051381:Rats; D011919:Rats, Inbred Strains; D000099182:Phosphoinositide 5-Phosphatases
D000906:Antibodies; D019269:Phosphatidylinositol 4,5-Diphosphate; D010716:Phosphatidylinositols; D010744:Phosphoric Monoester Hydrolases; D000099182:Phosphoinositide 5-Phosphatases