A mutant of herpes simplex virus type 1 exhibits increased stability of immediate-early (alpha) mRNAs.
vhs1 is a herpes simplex virus type 1 mutant defective in the shutoff of both host and alpha polypeptide synthesis. In cycloheximide reversal experiments, alpha mRNAs were significantly more stable in vhs1-infected cells than in cells infected with wild-type virus, whether assayed by in vitro translation or Northern blotting.
D000818:Animals; D003513:Cycloheximide / Q000494:pharmacology; D017874:Immediate-Early Proteins; D007700:Kinetics; D009154:Mutation; D012333:RNA, Messenger / Q000235:genetics*; D018139:Simplexvirus / Q000187:drug effects / Q000235:genetics*; D014709:Vero Cells; D014764:Viral Proteins / Q000235:genetics*
C050678:IE1 protein, Human herpesvirus 1; D017874:Immediate-Early Proteins; D012333:RNA, Messenger; D014764:Viral Proteins; D003513:Cycloheximide