Studies on the polyoma tumor-specific transplantation antigen (TSTA): selection and characterization of TSTA-negative segregants from somatic hybrids.
Two polyoma-TSTA-negative variants were selected independently from a polyoma fibrosarcoma/Moloney lymphoma somatic hybrid, by repeated passages in polyoma-virus-preimmunized mice. One of the variants had lost all its polyoma DNA, while the other only retained a deleted piece of its integrated polyoma DNA. In contrast to the parental hybrid clone, none of the variants produced detectable amounts of T-antigens. This finding indicates that a detectable expression of the products of the polyoma virus early genome, the T-antigens, is important either directly or indirectly for the expression of TSTA.
D000818:Animals; D000951:Antigens, Neoplasm / Q000032:analysis*; D002872:Chromosome Deletion; D002999:Clone Cells / Q000032:analysis; D004279:DNA, Viral / Q000032:analysis; D006649:Histocompatibility Antigens / Q000032:analysis*; D006822:Hybrid Cells / Q000032:analysis; D051379:Mice; D008808:Mice, Inbred CBA; D011120:Polyomavirus; D014412:Tumor Virus Infections / Q000032:analysis*
D000951:Antigens, Neoplasm; D004279:DNA, Viral; D006649:Histocompatibility Antigens; C029237:tumor-associated transplantation antigen