Morphological basis for multiple interactions of ethidium bromide (EB) with yeast Candida utilis.
Exposure of yeast cells to EB produced multiple effects on the cellular organelles: changes in the plasma membrane characterized by 75 to 110 nm deep pits; polymorphisms of the mitochondria ranging from cup-shaped, ring-shaped, ribbon-shaped, dumbbell-shaped structures to finally the formation of very elongated mitochondria (up to 4.5 micron in length); an increase in the length and number of endoplasmic reticulum; an increase in the number of cytoplasmic vesicles whose diameter varied between 25 to 45 nm. Furthermore, EB inhibited cytochrome c oxidase and cytochrome b biosynthesis, stimulated cytochrome c biosynthesis and uncoupled oxidative phosphorylation.
D002175:Candida / Q000187:drug effects* / Q000648:ultrastructure; D002462:Cell Membrane / Q000187:drug effects / Q000648:ultrastructure; D003573:Cytochrome b Group / Q000096:biosynthesis; D003574:Cytochrome c Group / Q000096:biosynthesis; D003576:Electron Transport Complex IV / Q000037:antagonists & inhibitors; D004721:Endoplasmic Reticulum / Q000187:drug effects / Q000648:ultrastructure; D004996:Ethidium / Q000494:pharmacology*; D008854:Microscopy, Electron; D008928:Mitochondria / Q000187:drug effects / Q000648:ultrastructure; D009940:Organoids / Q000187:drug effects* / Q000648:ultrastructure; D010085:Oxidative Phosphorylation / Q000187:drug effects; D010101:Oxygen Consumption / Q000187:drug effects; D011110:Polymorphism, Genetic
D003573:Cytochrome b Group; D003574:Cytochrome c Group; D003576:Electron Transport Complex IV; D004996:Ethidium