title: Expression of a germline human kappa chain-associated cross-reactive idiotype after in vitro and in vivo infection with Epstein-Barr virus.
abstract: The mouse monoclonal antibody 17.109 recognizes a cross-reactive idiotype (CRI) associated with kappa IIIb light chains of human IgM-rheumatoid factor (RF) paraproteins. The 17.109 idiotypic determinant is encoded by one or a group of closely related V kappa genes. The association of the idiotype with IgM- and IgA-rheumatoid factors in certain autoimmune diseases necessitates an understanding of how human B lymphocytes can be induced to express the idiotype. To investigate the cellular expression of the 17.109 CRI, peripheral blood lymphocytes from normal donors were stimulated in vitro with Epstein-Barr virus (EBV) and pokeweed mitogen (PWM). EBV induced greater expression of IgM-associated 17.109 CRI than did PWM. The 17.109 CRI was preferentially associated with IgM rather than with IgG. In vivo EBV infection was studied in college students with infectious mononucleosis and displayed similar elevation of IgM-associated 17.109 CRI in sera obtained at presentation of clinical illness. Later, IgM levels declined while IgG-associated 17.109 CRI rose. The 17.109 idiotype was unrelated to antibodies against the Epstein-Barr virus nuclear antigen and the viral capsid antigen and was probably due to generalized activation of early B cells. These observations support the hypothesis that the 17.109 CRI is expressed by in vitro and in vivo EBV-infected cells. The 17.109 idiotype identifies a highly conserved V kappa gene product, which is expressed preferentially after EBV infection, but not exclusively with RF autoantibodies.
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis; D000916:Antibody Diversity; D003429:Cross Reactions; D006509:Hepatitis B / Q000276:immunology; D004854:Herpesvirus 4, Human; D006801:Humans; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D007145:Immunoglobulin kappa-Chains / Q000276:immunology*; D007244:Infectious Mononucleosis / Q000276:immunology*; D008213:Lymphocyte Activation; D051379:Mice
chemical_list: D000911:Antibodies, Monoclonal; D007130:Immunoglobulin Idiotypes; D007145:Immunoglobulin kappa-Chains