Arginine-vasopressin stimulates the formation of phosphatidic acid in rat Leydig cells.
Arginine-vasopressin (AVP) stimulated the formation of labelled phosphatidic acid (PA) in [14C]arachidonic acid-prelabelled rat Leydig cells. After addition of 10(-6)M AVP [14C]arachidonoylphosphatidic acid reached a maximum within 2 min. The increase was dose-dependent (10(-11)-10(-6)M). No change in labelling of other phospholipids and diacylglycerol could be detected. The V1 antagonist dPTyr(Me)AVP inhibited in a dose-dependent manner the AVP-stimulated accumulation of PA. The V2 agonist dPVDAVP was without effect. The present results suggest that AVP binds to V1 receptors in rat Leydig cells resulting in stimulation of PA turnover. We suggest that the AVP-stimulated PA formation is an indication of phosphoinositide turnover.
D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism; D001127:Arginine Vasopressin / Q000031:analogs & derivatives / Q000494:pharmacology*; D007985:Leydig Cells / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D010712:Phosphatidic Acids / Q000096:biosynthesis*; D018129:Phosphatidylinositol Phosphates; D010716:Phosphatidylinositols / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D011945:Receptors, Angiotensin / Q000187:drug effects / Q000378:metabolism; D017483:Receptors, Vasopressin
D001095:Arachidonic Acids; D010712:Phosphatidic Acids; D018129:Phosphatidylinositol Phosphates; D010716:Phosphatidylinositols; D011945:Receptors, Angiotensin; D017483:Receptors, Vasopressin; D001127:Arginine Vasopressin; D016718:Arachidonic Acid; C028799:argipressin, deaminopenicillamine(1)-Val(4)-; C028888:argipressin, deaminopenicillamine(1)-O-methyl-Tyr(2)-