Expression of the art/trs protein of HIV and study of its role in viral envelope synthesis.
The art/trs transactivator protein of human immunodeficiency virus (HIV) was expressed in mammalian cells as a 19-kilodalton protein that was immunoreactive with sera from HIV-infected patients. Separate plasmids encoding the art/trs protein, the tat protein, or the envelope glycoprotein gp120 were used to demonstrate that both art/trs and tat are absolutely required for the synthesis of gp120 from its cognate messenger RNA. In addition, both the tat and art/trs proteins influence the level of envelope RNA. The results suggest that art/trs and tat may be ideal targets for potential anti-HIV agents in AIDS therapy.
D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology; D004262:DNA Restriction Enzymes; D015694:Gene Products, rev; D005796:Genes; D005814:Genes, Viral; D006678:HIV / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D010957:Plasmids; D012333:RNA, Messenger / Q000235:genetics; D014759:Viral Envelope Proteins / Q000096:biosynthesis*; D014764:Viral Proteins / Q000096:biosynthesis / Q000235:genetics*; D054321:rev Gene Products, Human Immunodeficiency Virus
D015694:Gene Products, rev; D012333:RNA, Messenger; D014759:Viral Envelope Proteins; D014764:Viral Proteins; D054321:rev Gene Products, Human Immunodeficiency Virus; D004262:DNA Restriction Enzymes