Suppression of matrix protein synthesis by herpes simplex virus in bovine smooth muscle cells.
We investigated the effect of herpes simplex virus type 1 infection on the synthesis of extracellular matrix proteins by bovine smooth muscle cells. The cells supported viral replication, which reached maximum at 24 hrs post-infection. Uninfected and infected (multiplicity of infection of 5 or 20) cultures of smooth muscle cells were labeled with [14C]proline at five hrs post-infection. Incorporation of radioactivity into non-dializable protein was determined following electrophoresis of the cell-matrix and medium fractions. The synthesis of fibronectin and collagen types I and III was almost completely suppressed. The data suggest that HSV-1 infection of smooth muscle cells in vitro alters extracellular matrix synthesis.
D000818:Animals; D001011:Aorta; D002417:Cattle; D002455:Cell Division; D002460:Cell Line; D002472:Cell Transformation, Viral; D005109:Extracellular Matrix / Q000378:metabolism*; D007700:Kinetics; D009124:Muscle Proteins / Q000037:antagonists & inhibitors* / Q000096:biosynthesis / Q000302:isolation & purification; D009131:Muscle, Smooth, Vascular / Q000378:metabolism*; D018139:Simplexvirus / Q000235:genetics*
D009124:Muscle Proteins