Effects of insulin on pyruvate dehydrogenase in circulating lymphocytes from normal and diabetic rats.
1. The in vivo and in vitro conditions which allow a response of rat circulating lymphocyte PDH to insulin are investigated. 2. In vivo tests show that inactive PDH (PDHi) prevails in diabetic rats and active PDH (PDHa) in hyperinsulinemic rats; in treated with insulin diabetic rats the PDHa/PDHi ratio (1.7) is similar to that of normal rats (PDHa/PDHi ratio = 2). 3. In vitro tests show a responsiveness of PDH to insulin only when 50 microM Ca2+ -Mg2+ and intact lymphocytes are used in the incubation medium. Insulin concentrations and contact time are important variables.
D000818:Animals; D002118:Calcium / Q000502:physiology; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000188:drug therapy* / Q000201:enzymology; D066298:In Vitro Techniques; D007328:Insulin / Q000494:pharmacology*; D007958:Leukocyte Count / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects* / Q000201:enzymology; D008274:Magnesium / Q000502:physiology; D008297:Male; D011768:Pyruvate Dehydrogenase Complex / Q000097:blood*; D051381:Rats
D007328:Insulin; D011768:Pyruvate Dehydrogenase Complex; D008274:Magnesium; D002118:Calcium