[Molecular cloning and study of the expression of a region of a diphtheria toxin gene encoding fragment A of the Streptomyces lividans 66 strain].
The shuttle plasmid pVG202 containing a part of diphtheria toxin gene coding for fragment A has been constructed. S. lividans strain 66 has been transformed by the plasmid pVG202 DNA. The presence of the hybrid plasmid in S. lividans 66 cells determines the production of catalytically active toxoid secreted into the cultural liquid medium. The deleted plasmid pVG205 which determines for the increased catalytic activity has been selected and shown to be stably inherited by the bacterial cells.
D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004167:Diphtheria Toxin / Q000235:genetics*; D005798:Genes, Bacterial; D009876:Operon; D010957:Plasmids; D011401:Promoter Regions, Genetic; D013302:Streptomyces / Q000235:genetics
D004167:Diphtheria Toxin; D004262:DNA Restriction Enzymes
Molekuliarnoe klonirovanie i izuchenie ékspressii chasti gena difteriĭnogo toksina, kodiruiushchego fragment A, v shtamme Streptomyces lividans 66.