Enrichment of complexing analytes on a copper-loaded silica surface and on-line coupling with high-performance liquid chromatography, using L-tryptophan and L-tyrosine as model compounds.
A chemically modified silica (bisdithiocarbamate) strongly binds copper and has a loading capacity of 0.5 mmol g-1 for the copper ion. This new metal-loaded silica, packed in a short stainless-steel pre-column, is highly selective for the enrichment of two amino acids chosen as model compounds. On-line coupling of this pre-column with an analytical RP-18 column allowed the separation and detection of L-tryptophan and L-tyrosine at parts per billion levels in aqueous media with good accuracy. Separation was achieved by ion-pair chromatography and the solutes were detected by fluorescence. The ions generally present in natural aqueous media did not interfere.
D002851:Chromatography, High Pressure Liquid; D003300:Copper; D007202:Indicators and Reagents; D012822:Silicon Dioxide; D012997:Solvents; D014364:Tryptophan / Q000302:isolation & purification*; D014443:Tyrosine / Q000302:isolation & purification*
D007202:Indicators and Reagents; D012997:Solvents; D014443:Tyrosine; D012822:Silicon Dioxide; D003300:Copper; D014364:Tryptophan