title: Telomeric site position heterogeneity in macronuclear DNA of Paramecium primaurelia.
abstract: In Paramecium primaurelia, the macronuclear gene encoding the G surface protein is located near a telomere. In this study, multiple copies of this telomere have been isolated and the subtelomeric and telomeric regions of some of them have been sequenced. The telomeric sequences consist of tandem repeats of the hexanucleotides C4A2 or C3A3. We show that the location where these repeats are added, which we call the telomeric site, is variable within a 0.6-0.8-kb region. These results are discussed in relation with the formation of macronuclear DNA.
mesh_terms: D000818:Animals; D001483:Base Sequence; D002467:Cell Nucleus / Q000648:ultrastructure*; D002875:Chromosomes / Q000648:ultrastructure*; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D004262:DNA Restriction Enzymes; D005796:Genes; D008562:Membrane Glycoproteins; D008565:Membrane Proteins / Q000235:genetics*; D010247:Paramecium / Q000235:genetics* / Q000648:ultrastructure; D015800:Protozoan Proteins
chemical_list: D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D015800:Protozoan Proteins; C051841:surface protein G, Paramecium; D004247:DNA; D004262:DNA Restriction Enzymes