title: Quantitative immunoelectron microscopic localization of (Na+,K+)ATPase on rat exocrine pancreatic cells.
abstract: Distribution of (Na+,K+)ATPase in rat exocrine pancreatic cells was investigated quantitatively by immunoelectron microscopy using the post-embedding protein A-gold technique. We found that in acinar and duct cells (Na+,K+)ATPase exists on both the luminal and the basolateral surfaces, with higher particle density on the luminal surface (4.4 times in the acinar cells and 5.6 times in the duct cells). According to Bolender (J Cell Biol 61:269, 1974), the luminal surface represents only 5% of the total cell surface of an average pancreatic acinar cell. It is roughly estimated, therefore, that approximately 80% of the plasma membrane (Na+,K+)ATPase in the acinar cells exists on the basolateral surface. When the acinar and duct cells were compared, more than twice as many particles were found on acinar cells than on duct cells. The enzyme existed on all the cell surfaces, preferentially on the microvilli or on the cell membrane folds, and no clustering was detected. We suggest that the (Na+,K+)ATPase on the basolateral surface is mainly responsible for the extrusion of a large number of sodium ions that are incorporated into the cytoplasm accompanying the secondary active transport of various organic substances and inorganic ions, whereas that on the luminal surface is responsible for active extrusion of sodium ions that are partially responsible for the fluid secretion of the pancreatic cells.
mesh_terms: D000818:Animals; D000906:Antibodies; D000918:Antibody Specificity; D002462:Cell Membrane / Q000201:enzymology; D006046:Gold; D007158:Immunologic Techniques; D007668:Kidney / Q000201:enzymology; D008297:Male; D008854:Microscopy, Electron / Q000379:methods*; D010179:Pancreas / Q000166:cytology / Q000201:enzymology* / Q000648:ultrastructure; D010183:Pancreatic Ducts / Q000201:enzymology / Q000648:ultrastructure; D051381:Rats; D011919:Rats, Inbred Strains; D000254:Sodium-Potassium-Exchanging ATPase / Q000276:immunology / Q000378:metabolism*
chemical_list: D000906:Antibodies; D006046:Gold; D000254:Sodium-Potassium-Exchanging ATPase