Reconstitution of membrane proteins: sequential incorporation of integral membrane proteins into preformed lipid bilayers.
Several integral membrane proteins can be inserted sequentially into preformed unilamellar vesicles (ULV's) composed of dimyristoylphosphatidylcholine (DMPC) and cholesterol in a gel phase. Thus, proteoliposomes of DMPC, cholesterol, and bacteriorhodopsin from Halobacterium halobium rapidly incorporate UDPglucuronosyltransferase (EC 2.4.1.17) from pig liver microsomes, cytochrome oxidase from beef heart mitochondria, and additional bacteriorhodopsin, added sequentially. This process of spontaneous incorporation can be regulated to produce complex artificial membranes that contain phospholipids and proteins at ratios (mol/mol) equivalent to what is found in biological membranes. The ability of the lipid-protein bilayers to incorporate additional integral membrane proteins is not affected by annealing of the proteoliposomes at 37 degrees C nor by the order of addition of the proteins. Bacteriorhodopsin-containing vesicles formed by the sequential addition of integral membrane proteins demonstrate light-driven proton pumping. Therefore, they have retained a vesicular structure. Vesicles containing one or two different proteins will fuse with each other at 21 degrees C or with ULV's devoid of proteins. Incorporation of bacteriorhodopsin or UDPglucuronosyltransferase into proteoliposomes containing DMPC, with or without cholesterol as impurity, also occurs above the phase transition for DMPC. The presence of a protein in a liquid-crystalline bilayer provides the necessary condition for promoting the spontaneous incorporation of other membrane proteins into preformed bilayers.
D000818:Animals; D001436:Bacteriorhodopsins / Q000378:metabolism*; D002417:Cattle; D002784:Cholesterol; D004134:Dimyristoylphosphatidylcholine; D003576:Electron Transport Complex IV / Q000378:metabolism*; D014453:Glucuronosyltransferase / Q000378:metabolism*; D006217:Halobacterium / Q000378:metabolism; D007700:Kinetics; D008051:Lipid Bilayers; D008565:Membrane Proteins / Q000378:metabolism*; D008862:Microsomes, Liver / Q000201:enzymology; D008929:Mitochondria, Heart / Q000201:enzymology; D013552:Swine
D008051:Lipid Bilayers; D008565:Membrane Proteins; D001436:Bacteriorhodopsins; D002784:Cholesterol; D003576:Electron Transport Complex IV; D014453:Glucuronosyltransferase; D004134:Dimyristoylphosphatidylcholine