A simple medium for the study of hepatocyte growth in culture under defined conditions.
The combination (1:1) of Dulbecco's modified Eagle's medium and Waymouth's medium MAB 87/3 was found to provide favorable conditions for serum-free culture and growth of adult rat hepatocytes. In this simple medium, a majority of hepatocytes stimulated by epidermal growth factor plus insulin entered S phase and divided, with a normal (13 h) interval between DNA synthesis and cell division. The proliferative response did not require extra substratum or the presence of serum, even during cell isolation and plating.
D000818:Animals; D002455:Cell Division; D002478:Cells, Cultured; D003094:Collagen / Q000502:physiology; D003470:Culture Media; D004247:DNA / Q000096:biosynthesis; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005109:Extracellular Matrix / Q000502:physiology; D007328:Insulin / Q000494:pharmacology; D008099:Liver / Q000166:cytology*; D008297:Male; D051381:Rats
D003470:Culture Media; D007328:Insulin; D004815:Epidermal Growth Factor; D003094:Collagen; D004247:DNA