title: Nosocomial outbreak of Legionnaires' disease: molecular epidemiology and disease control measures.
abstract: Molecular laboratory techniques were used to study the epidemiology of an outbreak of nosocomial Legionnaires' disease. All patient isolates were Legionella pneumophila serogroup 1 and showed identical plasmid profiles and reactions with serogroup-specific monoclonal antibodies. L pneumophila was also cultured from four of five cooling tower water samples; however, the isolate from only one tower was serogroup 1 of the same subtype as patient isolates. Since the cases were temporally clustered and epidemiologically associated with exposure to cooling tower aerosols, the single cooling tower implicated by molecular analysis was the most likely source of the outbreak. Chlorination of cooling tower ponds has eradicated the epidemic strain. Since potable water also harbored the infecting organism and was the probable source for cooling tower contamination, decontamination of the hospital water system was also undertaken. Superchlorination of hot water holding tanks to 17 ppm on a weekly basis has effectively eradicated L pneumophila from the potable water system and appears to be a reasonable, simple, and relatively inexpensive alternative to previously described methods of control.
mesh_terms: D003428:Cross Infection / Q000453:epidemiology* / Q000209:etiology / Q000517:prevention & control; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000032:analysis; D004196:Disease Outbreaks; D004587:Electrophoresis, Agar Gel; D004865:Equipment and Supplies, Hospital; D006785:Hospitals, University; D006801:Humans; D007875:Legionella / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D007877:Legionnaires' Disease / Q000453:epidemiology* / Q000209:etiology / Q000517:prevention & control; D010957:Plasmids; D012703:Serotyping; D014589:Utah; D014871:Water Microbiology; D014881:Water Supply
chemical_list: D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes