Pyrimidine dimer dependent cleavage of single-stranded DNA by T4 UV endonuclease.
T4 UV endonuclease cleaves double- and single-stranded DNA with equal specificity for photo-pyrimidine dimers. Thus, the enzyme can be used for mapping and quantifying pyrimidine dimers in single-stranded DNA as well as in double-stranded DNA. Mapping of pyrimidine dimers shows that rates of UV-dimerization are not only affected by 5', 3' adjacent bases, but also by position within pyrimidine tracts. Di-pyrimidines at 3' ends of tracts are more photoreactive than those at 5' ends.
D004260:DNA Repair; D004277:DNA, Single-Stranded / Q000378:metabolism*; D004279:DNA, Viral / Q000378:metabolism / Q000528:radiation effects; D004706:Endodeoxyribonucleases / Q000378:metabolism*; D011740:Pyrimidine Dimers / Q000378:metabolism*; D013539:Simian virus 40; D013379:Substrate Specificity; D013604:T-Phages / Q000201:enzymology; D014466:Ultraviolet Rays
D004277:DNA, Single-Stranded; D004279:DNA, Viral; D011740:Pyrimidine Dimers; D004706:Endodeoxyribonucleases