title: Comparison of MRC-5 and HFF cells for the identification of cytomegalovirus in centrifugation culture.
abstract: Detection of cytomegalovirus (CMV) by centrifugation culture was compared in MRC-5 and HFF cells. At 24 hr 47 of 130 specimens (36%) were positive for CMV, 45 in MRC-5, and 35 in HFF cells. At 48 hr 45 specimens were positive in both MRC-5 and HFF cells. MRC-5 cells appear to be superior for the detection of CMV at 24 hr in centrifugation culture; however, both lines are comparable at 48 hr.
mesh_terms: D002460:Cell Line; D002498:Centrifugation; D003587:Cytomegalovirus / Q000302:isolation & purification* / Q000502:physiology; D003588:Cytopathogenic Effect, Viral; D005347:Fibroblasts; D005455:Fluorescent Antibody Technique; D006801:Humans