Granulocyte-macrophage colony-stimulating factor. Sensitive and receptor-mediated regulation by 1,25-dihydroxyvitamin D3 in normal human peripheral blood lymphocytes.
We show that 1,25-dihydroxyvitamin D3 (1,25[OH]2D3), the most hormonally active metabolite of vitamin D3, modulates sensitively and specifically both the protein and messenger RNA accumulation of the multilineage growth factor granulocyte-macrophage colony-stimulating factor (GM-CSF). The regulation of GM-CSF expression is seen in both normal human mitogen-activated T lymphocytes and T lymphocytes from a line (S-LB1) transformed with human T cell lymphotropic virus 1 (HTLV-1). In contrast, cells from a HTLV-1 transformed T lymphocyte line (Ab-VDR) established from a patient with vitamin D-resistant rickets type II with undetectable 1,25(OH)2D3 cellular receptors are resistant to the action of 1,25(OH)2D3. Inhibition of GM-CSF expression by 1,25(OH)2D3 can occur independently of interleukin 2 regulation and is probably mediated through cellular 1,25(OH)2D3 receptors. We conclude that 1,25(OH)2D3 may be important in the physiology of hematopoiesis.
D002117:Calcitriol / Q000494:pharmacology*; D002472:Cell Transformation, Viral; D003115:Colony-Stimulating Factors / Q000096:biosynthesis*; D017977:Deltaretrovirus / Q000502:physiology; D003864:Depression, Chemical; D005786:Gene Expression Regulation / Q000187:drug effects*; D006098:Granulocytes; D006801:Humans; D007015:Hypophosphatemia, Familial / Q000473:pathology; D007376:Interleukin-2 / Q000494:pharmacology; D008264:Macrophages; D012333:RNA, Messenger / Q000096:biosynthesis; D018167:Receptors, Calcitriol; D011987:Receptors, Steroid / Q000502:physiology; D013601:T-Lymphocytes / Q000187:drug effects / Q000378:metabolism*
D003115:Colony-Stimulating Factors; D007376:Interleukin-2; D012333:RNA, Messenger; D018167:Receptors, Calcitriol; D011987:Receptors, Steroid; D002117:Calcitriol