High-performance liquid chromatography in protein sequence determinations.
The use of reversed-phase high-performance liquid chromatography (RP-HPLC) for the determination of protein sequences is reported. Topics considered include the peptide separation of endoprotease digestion mixtures, the application of HPLC peptide mapping as an efficient system to check the accuracy of an assumed protein sequence, obtained indirectly by DNA sequencing and the use of HPLC for amino acid analysis in the Edman sequence strategy. The use of RP-HPLC for an unconventional sequence strategy is demonstrated; HPLC exopeptidase mapping appears to be particularly useful as a future technique for small terminal sequence analysis. Finally, the coupling of HPLC with fast atom bombardment mass spectrometry is discussed.
D000085:Acetates; D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D006868:Hydrolysis; D013058:Mass Spectrometry; D010447:Peptide Hydrolases; D010449:Peptide Mapping; D011506:Proteins / Q000032:analysis*; D014269:Trifluoroacetic Acid
D000085:Acetates; D000596:Amino Acids; D011506:Proteins; D014269:Trifluoroacetic Acid; D010447:Peptide Hydrolases; C018824:ammonium acetate