Electroporation for the efficient transfection of mammalian cells with DNA.
A simple and reproducible procedure for the introduction of DNA into mammalian cells by electroporation is described. The parameters involving the cells, the DNA, and the electric field are investigated. The procedure has been applied to a broad range of animal cells. It is capable of transforming more than 1% of the viable cells to the stable expression of a selectable marker.
D000123:Acetyltransferases / Q000235:genetics; D001358:Avian Sarcoma Viruses / Q000235:genetics; D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics*; D004563:Electrochemistry; D005796:Genes; D005798:Genes, Bacterial; D005814:Genes, Viral; D005818:Genetic Engineering / Q000379:methods*; D005822:Genetic Vectors; D013006:Growth Hormone / Q000235:genetics; D006801:Humans; D019868:Kanamycin Kinase; D010430:Pentosyltransferases / Q000235:genetics; D010770:Phosphotransferases / Q000235:genetics; D010957:Plasmids
D004269:DNA, Bacterial; D013006:Growth Hormone; D000123:Acetyltransferases; D015500:Chloramphenicol O-Acetyltransferase; D010430:Pentosyltransferases; C024827:xanthine phosphoribosyltransferase; D010770:Phosphotransferases; D019868:Kanamycin Kinase