[Cloning and characteristics of recA gene in Pseudomonas aeruginosa].
A recA-like gene from Pseudomonas aeruginosa was cloned and identified by means of interspecific complementation of gene recA repair defect in Escherichia coli. The gene was mapped in the PvuII-HindIII Ps. aeruginosa chromosome fragment of 1.5 kbp in length. Having been recloned in pUC18 or 19 plasmids in either of possible orientations, this fragment was shown to complement three different defects of E. coli recA mutants: in repair, recombination and SOS functions.
D010582:Bacteriophage lambda / Q000235:genetics; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D010957:Plasmids; D011550:Pseudomonas aeruginosa / Q000235:genetics*; D011938:Rec A Recombinases / Q000235:genetics*; D013014:SOS Response, Genetics
D011938:Rec A Recombinases; D004262:DNA Restriction Enzymes
Klonirovanie i kharakteristika gena recA iz Pseudomonas aeruginosa.