title: Leishmania promastigotes are recognized by the macrophage receptor for advanced glycosylation endproducts.
abstract: In this paper we demonstrate the involvement of the macrophage receptor for advanced glycosylation endproducts (AGE) in the phagocytosis of Leishmania major promastigotes. Blocking of this receptor with the ligand, AGE-BSA, leads to a 50% decrease in phagocytosis relative to controls, and a comparable decrease in the respiratory burst. The inhibition of phagocytosis by AGE-BSA was specific to leishmania. The binding of zymosan or C3bi-RBC and the phagocytosis of IgG-RBC or latex beads was not affected by the presence of AGE-BSA. Blocking of both the AGE receptor and CR3 decreases leishmania binding by nearly 90%, and reduces the respiratory burst by 80%, indicating that the two receptors account for the bulk of L. tropica promastigote recognition and uptake by the macrophage.
mesh_terms: D000818:Animals; D001667:Binding, Competitive; D050260:Carbohydrate Metabolism; D006023:Glycoproteins / Q000378:metabolism*; D007895:Leishmania tropica / Q000166:cytology / Q000276:immunology*; D008264:Macrophages / Q000276:immunology* / Q000378:metabolism; D010587:Phagocytosis; D011956:Receptors, Cell Surface / Q000378:metabolism*
chemical_list: D006023:Glycoproteins; D011956:Receptors, Cell Surface