Multiple genes encode the human Na+,K+-ATPase catalytic subunit.
A human genomic library was constructed and screened with hybridization probes derived from sheep and rat cDNAs encoding the alpha and alpha(+) isoforms, respectively, of the Na+,K+-ATPase catalytic subunit. Genomic sequences spanning 150 kilobases were isolated. Four genes, designated alpha A, alpha B, alpha C, and alpha D, each 20-25 kilobases in length, were identified by restriction mapping, Southern blot hybridization analysis, and limited DNA sequencing. We present evidence that two of these genes, alpha A and alpha B, encode the alpha and alpha(+) isoforms, respectively. The other genes, alpha C and alpha D, one of which is physically linked to the alpha(+) gene, exhibit nucleotide and amino acid homology to Na+,K+-ATPase catalytic subunit cDNA sequences but do not correspond to any previously identified isoforms.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism; D004262:DNA Restriction Enzymes; D005796:Genes; D006801:Humans; D007668:Kidney / Q000201:enzymology; D007962:Leukocytes / Q000201:enzymology; D046911:Macromolecular Substances; D009693:Nucleic Acid Hybridization; D012756:Sheep; D000254:Sodium-Potassium-Exchanging ATPase / Q000097:blood / Q000235:genetics*
D046911:Macromolecular Substances; D004247:DNA; D004262:DNA Restriction Enzymes; D000254:Sodium-Potassium-Exchanging ATPase