Expression of tumor necrosis factor in vitro by human mononuclear phagocytes stimulated with whole Mycobacterium bovis BCG and mycobacterial antigens.
Four mycobacterial preparations directly stimulated human blood monocytes and alveolar macrophages to produce tumor necrosis factor (TNF). Monoclonal antibody against TNF blocked (99%) TNF activity. Lipopolysaccharide was not responsible for the TNF activity generated; activity was unaffected in the presence of polymyxin B.
D000942:Antigens, Bacterial / Q000276:immunology*; D001992:Bronchoalveolar Lavage Fluid; D006801:Humans; D066298:In Vitro Techniques; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D008050:Lipid A / Q000037:antagonists & inhibitors; D008262:Macrophage Activation; D008264:Macrophages / Q000276:immunology*; D009163:Mycobacterium bovis / Q000276:immunology*; D011112:Polymyxin B / Q000494:pharmacology; D013997:Time Factors; D014373:Tuberculin / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
D000942:Antigens, Bacterial; D008050:Lipid A; D014373:Tuberculin; D014409:Tumor Necrosis Factor-alpha; D011112:Polymyxin B