Identification of human papillomavirus type 18 E6 polypeptide in cells derived from human cervical carcinomas.
We recently reported the expression of human papillomavirus type 18 (HPV-18) E6 protein in bacteria and the production of anti-E6 polyclonal antibodies. This work has now been extended with the production of a panel of monoclonal antibodies against the HPV-18 E6 protein. These antibodies demonstrate that there is little antigenic conservation in the E6 protein between HPV-16 and HPV-18, with only one antibody recognizing a cross-reactive epitope. We have used both the monoclonal and the polyclonal antibodies to look for E6 expression in a number of HPV DNA-containing cell lines. These reagents specifically detected a 16.5K mol. wt. polypeptide in cells derived from a human cervical carcinoma.
D000911:Antibodies, Monoclonal / Q000276:immunology; D000912:Antibodies, Neoplasm / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology; D002277:Carcinoma / Q000032:analysis*; D002460:Cell Line; D004273:DNA, Neoplasm / Q000032:analysis; D004268:DNA-Binding Proteins; D005260:Female; D006801:Humans; D009363:Neoplasm Proteins / Q000302:isolation & purification*; D009856:Oncogene Proteins, Viral / Q000276:immunology / Q000302:isolation & purification*; D027383:Papillomaviridae / Q000145:classification / Q000276:immunology / Q000302:isolation & purification*; D011993:Recombinant Fusion Proteins / Q000276:immunology / Q000302:isolation & purification; D002583:Uterine Cervical Neoplasms / Q000032:analysis* / Q000382:microbiology
D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm; D000914:Antibodies, Viral; D004273:DNA, Neoplasm; D004268:DNA-Binding Proteins; C052603:E6 protein, Human papillomavirus type 18; D009363:Neoplasm Proteins; D009856:Oncogene Proteins, Viral; D011993:Recombinant Fusion Proteins