Phenotypic changes and gene expression in human colon mucosal epithelial cells upon transfection of a SV40 DNA-gpt recombinant.
Phenotypic changes (increased longevity, decreased growth factor requirements, altered cell surface features, growth in semisolid agarose, and SV40 T antigen expression) suggesting in vitro transformation were displayed by human normal colon mucosal epithelial cells transfected with pSV3gpt, a pBR322 recombinant containing the SV40 "early" T antigen coding region and the dominant selectable marker bacterial gene, xanthine-guanine phosphoribosyltransferase. In contrast, control cultures which received neither DNA nor the recombinant pSV2gpt (which is identical to pSV3gpt but lacks the SV40 T antigen region) were not phenotypically altered.
D000956:Antigens, Viral / Q000235:genetics; D002471:Cell Transformation, Neoplastic; D002472:Cell Transformation, Viral; D003110:Colonic Neoplasms / Q000235:genetics / Q000276:immunology / Q000473:pathology*; D004274:DNA, Recombinant; D004279:DNA, Viral / Q000235:genetics; D004848:Epithelium / Q000473:pathology; D005786:Gene Expression Regulation; D006801:Humans; D007413:Intestinal Mucosa / Q000473:pathology; D010641:Phenotype; D013539:Simian virus 40 / Q000235:genetics*; D014162:Transfection
D000956:Antigens, Viral; D004274:DNA, Recombinant; D004279:DNA, Viral