[Localization of neutral Mn-dependent DNAse in rat hepatocytes. An immunofluorescent study].
Rabbit antibodies against the Mn-dependent DNAse were obtained. The specificity of the anti-DNAse antibody was established by the enzyme inhibition in vitro. The enzyme activity was inhibited by more than 75 and 54% using rabbit antisera and affinity-purified IgG. Localization of the enzyme in the rat hepatocyte nuclei was studied by indirect immunofluorescence.
D000818:Animals; D000906:Antibodies / Q000032:analysis; D000918:Antibody Specificity; D002467:Cell Nucleus / Q000201:enzymology; D003851:Deoxyribonucleases / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification; D005455:Fluorescent Antibody Technique; D008099:Liver / Q000201:enzymology*; D008345:Manganese / Q000494:pharmacology; D051381:Rats
D000906:Antibodies; D008345:Manganese; D003851:Deoxyribonucleases
Lokalizatsiia neĭtral'noĭ Mn-zavisimoĭ DNKazy v gepatotsitakh krys. Immunofluorestsentnoe issledovanie.