Isolation and preliminary characterization of temperature-sensitive mutants of human cytomegalovirus.
Thirteen temperature-sensitive (ts) mutants of human cytomegalovirus (HCMV) have been isolated after mutagenesis with nitrosoguanidine or ultraviolet light. Four mutants defective in viral DNA synthesis at nonpermissive temperature (DNA-) were classified into two separate complementation groups. The other nine HCMV ts mutants, capable of synthesizing viral DNA at the elevated temperature (DNA+), belonged to seven independent complementation groups. The number of essential genes encoded by HCMV is thus raised here by nine.
D003587:Cytomegalovirus / Q000235:genetics* / Q000302:isolation & purification; D004279:DNA, Viral / Q000096:biosynthesis; D005814:Genes, Viral; D005816:Genetic Complementation Test; D006801:Humans; D009154:Mutation; D013696:Temperature
D004279:DNA, Viral