Identification of enterotoxigenic Escherichia coli isolates with enzyme-labeled synthetic oligonucleotide probes.
Commercially available kits containing alkaline phosphatase-labeled oligonucleotide probes for Escherichia coli heat-stable enterotoxins (STI-H, STI-P, and STII) and the heat-labile enterotoxin were compared with bioassays and radiolabeled recombinant DNA probes to identify enterotoxigenic E. coli from 100 clinical isolates. There was very good agreement between the three methods.
D000469:Alkaline Phosphatase; D001427:Bacterial Toxins / Q000235:genetics*; D015342:DNA Probes; D004768:Enterotoxins / Q000235:genetics*; D004926:Escherichia coli / Q000302:isolation & purification*; D029968:Escherichia coli Proteins; D011933:Reagent Kits, Diagnostic
D001427:Bacterial Toxins; D015342:DNA Probes; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D011933:Reagent Kits, Diagnostic; C034151:heat stable toxin (E coli); C027538:heat-labile enterotoxin, E coli; D000469:Alkaline Phosphatase