[In vivo bactericidal activity of an oxacillin-netilmicin combination against Staphylococcus aureus. Influence of the rhythm of netilmicin administration].
The bactericidal activity of two regimens of netilmicin (8 mg/kg/day) given intravenously once a day (od) or thrice daily (tid) both alone and in combination with oxacillin (200 mg/kg/day) was compared using a model of fibrin clots infected with a strain of Staphylococcus aureus (10(7) CFU/g) sensitive to methicillin and netilmicin (clinical isolate) and implanted subcutaneously in rabbit. This study shows that: 1) Netilmicin given alone as both single and divided doses results in early bacterial killing but does not exert a bactericidal effect after 24 hours because of a significant late increase of the number of bacterial. 2) The netilmicin-oxacillin combinations are more bactericidal at 1 h, 2 h and 24 h than oxacillin alone (P less than 0.001). 3) The oxacillin-netilmicin combination appears to be better for bacterial killing when netilmicin is given thrice daily (P less than 0.001). It is hard to draw a clinical inference from such an experimental study but it seems that 8-hour divided doses intervals should be better for administration of netilmicin than single daily dose during the acute period of staphylococcal infections.
D000818:Animals; D004334:Drug Administration Schedule; D004359:Drug Therapy, Combination; D005337:Fibrin; D007262:Infusions, Intravenous; D007275:Injections, Intravenous; D009428:Netilmicin / Q000008:administration & dosage* / Q000097:blood / Q000493:pharmacokinetics; D010068:Oxacillin / Q000008:administration & dosage* / Q000097:blood / Q000493:pharmacokinetics; D011817:Rabbits; D013203:Staphylococcal Infections / Q000188:drug therapy*; D013211:Staphylococcus aureus / Q000187:drug effects; D013997:Time Factors
D009428:Netilmicin; D005337:Fibrin; D010068:Oxacillin
Etude de l'activité bactéricide in vivo de l'association oxacilline-nétilmicine vis-à-vis de Staphylococcus aureus. Influence du rythme d'administration de la nétilmicine.