Levels of glycerate 2,3-P2, 2,3-bisphosphoglycerate synthase and 2,3-bisphosphoglycerate phosphatase activities in rat tissues. A method to quantify blood contamination of tissue extracts.
The levels of glycerate 2,3-P2 and of 2,3-bisphosphoglycerate synthase and 2,3-bisphosphoglycerate phosphatase activities have been determined in isolated rat hepatocytes and adipocytes and in perfused rat tissues to discard blood contamination. The values obtained are much lower than those previously reported, ranging 0.50-40 nmol/g tissue. No relationship appears to exist between glycerate 2,3-P2 concentration and the levels of the enzymatic activities involved in glycerate 2,3-P2 metabolism. Assay of glycerate 2,3-P2 in tissue extracts constitute a very useful way to quantify blood contamination.
D019794:2,3-Diphosphoglycerate; D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D004163:Diphosphoglyceric Acids / Q000097:blood / Q000378:metabolism*; D008099:Liver / Q000378:metabolism; D008297:Male; D009713:Nucleotidyltransferases / Q000378:metabolism*; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013430:Sulfate Adenylyltransferase / Q000378:metabolism*; D014018:Tissue Distribution
D004163:Diphosphoglyceric Acids; C019794:bisphosphoglycerate phosphatase; D019794:2,3-Diphosphoglycerate; D009713:Nucleotidyltransferases; D013430:Sulfate Adenylyltransferase; D010744:Phosphoric Monoester Hydrolases