Interactions between the transposable element IS21 on R68.45 and TN7 in Pseudomonas aeruginosa PAO.
Tn7 transposes from the chromosome of Pseudomonas aeruginosa into the plasmid R68.45 with tandem IS21, at up to 400 times the frequency that it transposes into R68, which has only one copy of IS21. While R68::TN7 derivatives are stable, R68.45::Tn7 isolates undergo frequent deletions. Instability of R68.45 occurs whether Tn7 is inserted into the plasmid (cis configuration) or into the bacterial chromosome (trans configuration). The deletions of R68.45 start at the junction between the tandem IS21 copies and proceed clockwise, ending in the region of oriT. It appears that Tn7 and IS21 can mutually stimulate transposition of each other.
D002872:Chromosome Deletion; D002874:Chromosome Mapping; D004262:DNA Restriction Enzymes; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics; D005786:Gene Expression Regulation; D007612:Kanamycin; D011550:Pseudomonas aeruginosa / Q000235:genetics*; D011815:R Factors; D013752:Tetracycline
D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D007612:Kanamycin; D004262:DNA Restriction Enzymes; D013752:Tetracycline