Immunocytochemical demonstration of cyclic AMP-dependent protein kinases in duct cells of the rat parotid gland.
The cyclic AMP-dependent protein kinases were immunolocalized in the rat parotid gland using a monospecific antiserum against their catalytic subunit. The kinases were found to be primarily located in the cytoplasm of the parotid duct cells with a preference for the apical cell region. The result questions the traditional view of the control of parotid gland secretion and suggests a role of cyclic AMP not only in the acinar protein secretion but also in ductal functions like fluid and electrolyte transport.
D000818:Animals; D000242:Cyclic AMP / Q000378:metabolism*; D006651:Histocytochemistry; D066298:In Vitro Techniques; D008297:Male; D010306:Parotid Gland / Q000166:cytology / Q000201:enzymology*; D011494:Protein Kinases / Q000032:analysis* / Q000276:immunology; D051381:Rats; D011919:Rats, Inbred Strains
D000242:Cyclic AMP; D011494:Protein Kinases