Abelson virus transformation of an interleukin 2-dependent antigen-specific T-cell line.
Abelson murine leukemia virus (A-MuLV) carries the gene v-abl, one of a group of oncogenes with structural and functional (tyrosine kinase) homology to three growth factor receptors. Work in this and other laboratories has shown that A-MuLV infection can render myeloid and lymphoid cells independent of the growth factors interleukin 3 and granulocyte-macrophage colony-stimulating factor. We have now shown that v-abl can also relieve interleukin 2 (IL-2) dependence in T cells. We infected a cloned IL-2-dependent antigen-specific cell line. Transformed cells were generated which were factor independent and tumorigenic. The transformants each bore unique v-abl DNA inserts and expressed v-abl mRNA. No elevation of expression of either IL-2 or its receptor could be detected in these cells. Thus, A-MuLV can short-circuit the dependence of hematopoietic cells on IL-2, IL-3, and possibly granulocyte-macrophage colony-stimulating factor, none of whose receptors are known to be of the tyrosine kinase type.
D000011:Abelson murine leukemia virus / Q000235:genetics*; D000818:Animals; D002460:Cell Line; D002472:Cell Transformation, Viral; D007376:Interleukin-2 / Q000235:genetics*; D009052:Leukemia Virus, Murine / Q000235:genetics*; D009857:Oncogenes; D012333:RNA, Messenger / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D011971:Receptors, Immunologic / Q000235:genetics / Q000378:metabolism; D015375:Receptors, Interleukin-2; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism / Q000382:microbiology
D007376:Interleukin-2; D012333:RNA, Messenger; D012367:RNA, Viral; D011971:Receptors, Immunologic; D015375:Receptors, Interleukin-2