title: Neutrophil function in localized juvenile periodontitis. Phagocytosis, superoxide production and specific granule release.
abstract: Patients with localized juvenile periodontitis (LJP) exhibit defective neutrophil functions to a variety of environmental and host stimuli. It is not clear, however, how many of the measurable functions are defective and whether individual patients exhibit single or multiple dysfunctions. The purpose of this study was to evaluate chemotaxis, phagocytosis, specific granule release and superoxide production in a group of 23 previously unreported LJP patients. Our results indicate that all 23 of these LJP patients exhibited chemotaxis depression to N-formyl-L-methionyl-L-leucyl-L-phenylalanine (FMLP) and endotoxin-activated serum (EAS). Smaller groups from the 23 chemotactically defective LJP group were used to test other function due to inability to obtain sufficient quantities of blood. Fourteen of 14 LJP patients tested exhibited defective phagocytosis. Ten LJP patients were evaluated for specific granule release, and 14 LJP patients were evaluated for superoxide production. Both granule release and superoxide production were found to be normal in chemotactically defective LJP patients. Since both defective and normal responses noted in the same neutrophil populations are mediated by the same receptor, it is hypothesized that the cellular defect lies in a post receptor pathway.
mesh_terms: D010520:Aggressive Periodontitis / Q000378:metabolism / Q000503:physiopathology*; D002634:Chemotaxis, Leukocyte; D003594:Cytoplasmic Granules / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D007781:Lactoferrin / Q000378:metabolism*; D007782:Lactoglobulins / Q000378:metabolism*; D009504:Neutrophils / Q000378:metabolism / Q000502:physiology*; D010510:Periodontal Diseases / Q000503:physiopathology*; D010587:Phagocytosis; D011951:Receptors, Complement / Q000502:physiology; D013481:Superoxides / Q000096:biosynthesis*
chemical_list: D007782:Lactoglobulins; D011951:Receptors, Complement; D013481:Superoxides; D007781:Lactoferrin