Increased adenylate cyclase activity in rat thyroid epithelial cells expressing viral ras genes.
The activity of the adenylate cyclase catalytic subunit is higher in Harvey and Kirsten Murine Sarcoma Viruses-infected thyroid epithelial cells than in uninfected control cells either in the presence of Mg2+ alone or following stimulation by Mn2+ or forskolin. The higher activity is associated with an increased cAMP cellular content. The Gpp(NH)p and F- anion are more effective positive modulators in the control than in the virus infected cells: these results exclude therefore that the ras p21 proteins can act as the G-protein alpha-subunit and suggest that they negatively interfere with the G-protein modulation of the adenylate cyclase system.
D000262:Adenylyl Cyclases / Q000032:analysis*; D000818:Animals; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000032:analysis; D004848:Epithelium / Q000201:enzymology; D006165:Guanylyl Imidodiphosphate / Q000494:pharmacology; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D008345:Manganese / Q000494:pharmacology; D015689:Oncogene Protein p21(ras); D009856:Oncogene Proteins, Viral / Q000502:physiology; D009857:Oncogenes; D051381:Rats; D011916:Rats, Inbred F344; D012969:Sodium Fluoride / Q000494:pharmacology; D013961:Thyroid Gland / Q000201:enzymology*
D009856:Oncogene Proteins, Viral; D005576:Colforsin; D006165:Guanylyl Imidodiphosphate; D008345:Manganese; D012969:Sodium Fluoride; D000242:Cyclic AMP; D015689:Oncogene Protein p21(ras); D000262:Adenylyl Cyclases; D008274:Magnesium