title: A bacterial factor induces changes in cysteine proteinase forms in the cellular slime mould Dictyostelium discoideum.
abstract: The electrophoretic pattern of cysteine proteinases in axenically grown myxamoebae of Dictyostelium discoideum can be altered by the addition of either Gram-negative (Klebsiella aerogenes, Escherichia coli) or Gram-positive (Micrococcus lysodeikticus, Bacillus subtilis) bacteria to the culture. No changes occurred, however, if either yeast or latex beads were used in place of bacteria. The changes involved the simultaneous loss of proteinases characteristic of the axenic cells (the A-forms) and the acquisition of those found in cells which have been grown on bacteria (the B-forms). Using K. aerogenes the conversion was complete within 4 h. Extracellular proteinase activity was unaffected during this period. After the D. discoideum cells had been lysed, no equivalent change in proteinase band pattern could be produced either by prolonged incubation of cell extracts or by treatment with proteinases. An identical conversion could be induced in cultures of myxamoebae by a factor, cysteine proteinase converting factor (CPCF), present in the 15,000 g supernatant of a sonicated suspension of K. aerogenes. CPCF was macromolecular, as demonstrated by both ultrafiltration and gel filtration, acid-precipitable, but was soluble in ethanol or alkali. Its activity was unaffected by treatment with trypsin. The results suggested that CPCF might be a component of the bacterial cell wall, and since its activity was affected by lysozyme treatment, peptidoglycan is implicated. The results can be interpreted in terms of a novel nutrient-dependent post-translational change which affected most of the cysteine proteinases present in D. discoideum myxamoebae.
mesh_terms: D001419:Bacteria / Q000378:metabolism*; D002473:Cell Wall / Q000378:metabolism; D002918:Chymotrypsin / Q000494:pharmacology; D003546:Cysteine Endopeptidases / Q000378:metabolism*; D004023:Dictyostelium / Q000187:drug effects / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel; D007527:Isoenzymes / Q000378:metabolism*; D007711:Klebsiella pneumoniae / Q000378:metabolism; D010206:Papain / Q000494:pharmacology; D010457:Peptidoglycan / Q000494:pharmacology; D011480:Protease Inhibitors / Q000494:pharmacology
chemical_list: D007527:Isoenzymes; D010457:Peptidoglycan; D011480:Protease Inhibitors; D002918:Chymotrypsin; D003546:Cysteine Endopeptidases; D010206:Papain