The effect of growth conditions on production and excretion of extracellular antigens by three ascomycetous yeasts.
Ascomycetous yeasts produce extracellular antigens that are almost specific for the species. The antigen production by Hansenula wickerhamii and Stephanoascus ciferrii was independent of the carbon source and was proportional to the final cell density of the cultures. The same was true of chemostat cultures of Stephanoascus ciferrii, irrespective of the dilution rate and whether glucose or ammonia was the limiting nutrient. In cultures of Saccharomyces cerevisiae, however, antigen excretion mainly took place in the late exponential growth phase. Large amounts of antigen were extracted from the cell wall of Saccharomyces cerevisiae. A small amount was detected in the cytoplasm.
D000641:Ammonia / Q000378:metabolism; D000946:Antigens, Fungal / Q000302:isolation & purification / Q000378:metabolism*; D000954:Antigens, Surface / Q000302:isolation & purification; D002175:Candida / Q000254:growth & development / Q000276:immunology*; D002244:Carbon / Q000378:metabolism; D002473:Cell Wall / Q000276:immunology; D003470:Culture Media; D003593:Cytoplasm / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D008828:Microbiological Techniques; D010843:Pichia / Q000254:growth & development / Q000276:immunology*; D011134:Polysaccharides / Q000276:immunology; D011523:Protoplasts / Q000276:immunology; D012441:Saccharomyces cerevisiae / Q000254:growth & development / Q000276:immunology*; D004718:Saccharomycetales / Q000276:immunology*
D000946:Antigens, Fungal; D000954:Antigens, Surface; D003470:Culture Media; D011134:Polysaccharides; D002244:Carbon; D000641:Ammonia