Activity of deoxyribonucleoside-activated nucleotidase in cells from various lymphoid mouse tissues and in concanavalin A-stimulated lymphocytes.
The specific activity in cells from lymph nodes, spleen and thymus was 32, 28 and 25 nmol/min per mg of cytosol protein, respectively, whereas that in bone marrow cells was significantly lower (10 units/mg of protein). No difference in specific DAN activity between isolated B- and T-lymphocytes was observed. Two types of lymphoid mouse cell lines (MOPC-31C plasmacytoma cells, S49 Cyc- lymphoma cells) showed specific activities similar to the normal lymphoid cells. In concanavalin A- stimulated spleen lymphocytes in culture there was a rapid increase in DAN activity shortly after maximum DNA synthesis, reaching a plateau 2-3 times the original level. The enzyme (DAN) of mouse tissues possessed the characteristic properties previously detected for the rat enzyme.
D000818:Animals; D001853:Bone Marrow / Q000201:enzymology*; D002460:Cell Line; D003208:Concanavalin A; D003600:Cytosol / Q000201:enzymology; D007700:Kinetics; D008198:Lymph Nodes / Q000201:enzymology*; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000201:enzymology* / Q000276:immunology; D008223:Lymphoma / Q000201:enzymology; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009708:Nucleotidases / Q000378:metabolism*; D009928:Organ Specificity; D010954:Plasmacytoma / Q000201:enzymology; D013154:Spleen / Q000201:enzymology*; D013950:Thymus Gland / Q000201:enzymology*
D003208:Concanavalin A; D009708:Nucleotidases; C030287:nucleotidase