Detergent inhibition of nitric-oxide reductase activity.
Gas chromatography revealed that exposure of extracts of the denitrifiers 'Achromobacter cycloclastes', Paracoccus denitrificans, Pseudomonas aeruginosa and Pseudomonas perfectomarina to Triton X-100 inhibited reduction of NO to N2O, and thus concomitantly inhibited reduction of NO2- to N2O. After exposure of extracts to Triton X-100, the ratio of H+ consumed to NO2- added decreased from approx. 2.0 (for untreated extracts) to approx. 1.5, which indicated that NO2- was reduced to NO by the treated extracts. Addition of a CHAPS-soluble extract (devoid of nitrite reductase activity but rich in nitric-oxide reductase activity) to the Triton X-100-treated extract of P. denitrificans restored capacity for reduction of NO2- on to N2O. Exposure to either the NO that accumulated from reduction of NO2- or to enthetic NO transiently inhibited rates of NO2- reduction in Triton X-100-treated extracts. Use of an Oxides of Nitrogen analyzer indicated that only 5-33% of NO2- reduced by untreated extracts appeared in the stripping gas as NO, whereas 80-95% of NO2- reduced by Triton X-100-treated extracts was recovered as NO.
D000421:Alcaligenes / Q000201:enzymology; D002793:Cholic Acids / Q000494:pharmacology; D002849:Chromatography, Gas; D003902:Detergents / Q000494:pharmacology*; D017830:Octoxynol; D010088:Oxidoreductases / Q000037:antagonists & inhibitors*; D010231:Paracoccus denitrificans / Q000201:enzymology; D011092:Polyethylene Glycols / Q000494:pharmacology; D011522:Protons; D011549:Pseudomonas / Q000201:enzymology; D013501:Surface-Active Agents / Q000494:pharmacology*
D002793:Cholic Acids; D003902:Detergents; D011522:Protons; D013501:Surface-Active Agents; D011092:Polyethylene Glycols; D017830:Octoxynol; D010088:Oxidoreductases; C034747:nitric-oxide reductase; C028213:3-((3-cholamidopropyl)dimethylammonium)-1-propanesulfonate