[Study of the interaction between cytochrome c and cytochrome oxidase by tritium planigraphy].
Treatment of molecular crystals of the bovine cytochrome oxidase and the cytochrome oxidase-cytochrome c complex with thermally activated tritium leads to highly labelled cytochrome oxidase preparations. HPLC separation of its subunits and measurements of radioactivity of each polypeptide allow to determine the shielding of cytochrome oxidase surface sites by cytochrome c in the complex.
D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D003574:Cytochrome c Group / Q000378:metabolism*; D003576:Electron Transport Complex IV / Q000378:metabolism*; D008954:Models, Biological; D009206:Myocardium / Q000201:enzymology; D010455:Peptides / Q000378:metabolism; D013379:Substrate Specificity; D014316:Tritium
D003574:Cytochrome c Group; D010455:Peptides; D014316:Tritium; D003576:Electron Transport Complex IV
Issledovanie vzaimodeĭstviia tsitokhroma c s tsitokhromoksidazoĭ metodom tritievoĭ planigrafii.