Germination of Saccharomyces cerevisiae ascospores without trehalose mobilization as revealed by in vivo 13C nuclear magnetic resonance spectroscopy.
Saccharomyces cerevisiae ascospores germinate in the presence of acetate without any detectable trehalose degradation, as revealed by high-resolution nuclear magnetic resonance spectroscopy and by a standard colorimetric assay. The results presented here substantiate the hypothesis that in S. cerevisiae trehalose supplies energy during dormancy of the spores and not during the germination process.
D000085:Acetates / Q000378:metabolism; D002247:Carbon Isotopes; D003470:Culture Media; D004187:Disaccharides / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D012441:Saccharomyces cerevisiae / Q000502:physiology*; D013172:Spores, Fungal / Q000502:physiology; D014199:Trehalose / Q000378:metabolism*
D000085:Acetates; D002247:Carbon Isotopes; D003470:Culture Media; D004187:Disaccharides; D014199:Trehalose; D005947:Glucose