Oscillation of ion fluxes in mammalian erythrocytes. Mechanism of oscillation.
The dependence of ionophore-induced oscillations in rat erythrocytes on various concentrations of A23187, FCCP and Ca2+ was analysed using ion-selective electrodes. The oscillations were shown to be independent of the extracellular concentration of carbonylcyanide p-trifluoromethoxyphenylhydrazone and Ca2+. The dependence of oscillations on the concentration A23187 was shown to be a threshold characteristic and represented by a bell-shaped curve. In the course of oscillations the redistribution of A23187 between cells and the incubation medium was demonstrated using high-speed centrifugation. A hypothesis for oscillatory-state generation in erythrocytes was suggested on the basis of pH-dependent changes of the Ca2+ ionophore A23187 content in cells. According to this hypothesis the H+ concentration within the external membrane-adjacent layer serves as a causative factor for induction of cyclic desorption of A23187 molecules from the cell membrane.
D000818:Animals; D000001:Calcimycin / Q000097:blood / Q000494:pharmacology; D002118:Calcium / Q000097:blood / Q000494:pharmacology; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone / Q000494:pharmacology; D004573:Electrolytes / Q000097:blood*; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism*; D066298:In Vitro Techniques; D010507:Periodicity; D011188:Potassium / Q000097:blood; D011522:Protons; D051381:Rats; D011919:Rats, Inbred Strains
D004573:Electrolytes; D011522:Protons; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone; D000001:Calcimycin; D011188:Potassium; D002118:Calcium