Enzymatic conversion of leukotriene B4 to 6-trans-leukotriene B4 by rat kidney homogenates.
A novel isomerase reaction leading to conversion of leukotriene B4 to its 6-trans isomer was detected in rat kidney homogenates. The structure of the metabolite was determined by high performance liquid chromatography, ultraviolet spectrometry and gas-liquid chromatography-mass spectrometry. A recent report has shown that 6-trans-leukotriene B4 is transformed to a dihydro metabolite (6,7- or 10,11-dihydro 6-trans-leukotriene B4) and further omega-hydroxylated [Powell, W.S. (1986) Biochem, Biophys. Res. Commun. 136, 707-712]. The leukotriene B4 6-isomerase reaction reported here may therefore provide the first step in a novel pathway of biological degradation of leukotriene B4.
D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D007535:Isomerases / Q000378:metabolism*; D007668:Kidney / Q000201:enzymology*; D007975:Leukotriene B4 / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013056:Spectrophotometry, Ultraviolet; D019745:cis-trans-Isomerases
D007975:Leukotriene B4; D007535:Isomerases; D019745:cis-trans-Isomerases; C051520:leukotriene B4 6-isomerase