A rapid and precise assay for calmodulin and cyclic AMP phosphodiesterase on a centrifugal analyser.
An assay for cyclic AMP phosphodiesterase in biological samples is described. The method is a continuous, spectrophotometric assay adapted for use on a centrifugal analyser. Using the calmodulin-activated cyclic AMP phosphodiesterase prepared from pig brain by DEAE-cellulose chromatography, and a standard calmodulin preparation from the same source, a rapid assay for calmodulin is described. The calmodulin assay has a working range of 2 to 6 mg/l calmodulin in the sample. The within-batch CV is less than 5% and the between-batch CV is 7.0% or less.
D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000032:analysis*; D000302:Adrenal Cortex / Q000032:analysis / Q000201:enzymology; D000818:Animals; D001921:Brain / Q000201:enzymology; D001923:Brain Chemistry; D002147:Calmodulin / Q000032:analysis*; D002417:Cattle; D002498:Centrifugation / Q000295:instrumentation; D002848:Chromatography, DEAE-Cellulose; D013552:Swine
D002147:Calmodulin; D015105:3',5'-Cyclic-AMP Phosphodiesterases