Identification of an Epstein-Barr virus-specific desoxyribonuclease gene using complementary DNA.
We have recently obtained 18 distinct cDNA clones representing different genes expressed in the early phase of EBV infection. One of them, c37, which is situated at the position 12907-122451 in the B95-8 viral genome, is shown here to code for a viral desoxyribonuclease [DNase]. Cell free translation of c37-selected messenger RNA yielded a protein of about 52 KDa which was immunoprecipitated by a high EA titer serum from nasopharyngeal carcinoma patient. This protein showed a DNase activity which was resistant to high salt concentrations (150 to 300 mM KCl) and was specifically neutralized by EA positive serum. These properties are typical of the EBV-specific DNase activity that we recently described in chemically induced EBV-transformed lymphoid cells. The same results were obtained on cell-free translation of the native RNA synthesized in vitro from pGEM-37 plasmid containing the entire c37 cDNA sequence (1.53 Kb). These data indicate that the BGLF5 open reading frame contained in c37 encodes for the EBV-specific DNase.
D001483:Base Sequence; D002460:Cell Line; D002472:Cell Transformation, Viral; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism*; D003851:Deoxyribonucleases / Q000235:genetics*; D005796:Genes; D005814:Genes, Viral; D004854:Herpesvirus 4, Human / Q000201:enzymology / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D014176:Protein Biosynthesis; D014158:Transcription, Genetic
D004247:DNA; D003851:Deoxyribonucleases