title: The action of a water-soluble carbodiimide on adenosine-5'-polyphosphates.
abstract: When aqueous solutions of adenosine-5'-mono-, di-, or triphosphates are treated with a water soluble carbodiimide the major product is the expected diadenosine-5'-5'-polyphosphate. The yields of these pyrophosphates are greatly increased in the presence of the Mg2+ ion. Adenosine-5'-tetraphosphate behaves differently. The major product is adenosine-5'-monophosphate. We believe that this hydrolysis occurs via a cyclic trimetaphosphate intermediate.
mesh_terms: D000227:Adenine Nucleotides / Q000378:metabolism*; D000469:Alkaline Phosphatase; D003028:CME-Carbodiimide / Q000031:analogs & derivatives / Q000494:pharmacology*; D002234:Carbodiimides / Q000494:pharmacology*; D005022:Ethyldimethylaminopropyl Carbodiimide / Q000494:pharmacology*; D006868:Hydrolysis; D043264:Phosphodiesterase I; D010727:Phosphoric Diester Hydrolases; D012995:Solubility; D014867:Water
chemical_list: D000227:Adenine Nucleotides; D002234:Carbodiimides; D014867:Water; D003028:CME-Carbodiimide; D000469:Alkaline Phosphatase; D010727:Phosphoric Diester Hydrolases; D043264:Phosphodiesterase I; D005022:Ethyldimethylaminopropyl Carbodiimide