title: Increased synthesis of human parathyroid hormone in Escherichia coli through alterations of the 5' untranslated region.
abstract: The expression of human parathyroid hormone (hPTH) in Escherichia coli was optimized by variations of the spacing sequence between the ribosome-binding site (RBS) and the beginning of the gene (ATG) and by increasing the complementarity of the RBS to the 16 S rRNA. The expression level of 3 micrograms/liter increased more than 100-fold to 475 micrograms/liter as a direct consequence of modifications in the region 5' of the gene.
mesh_terms: D001483:Base Sequence; D004275:DNA, Ribosomal / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D005796:Genes; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D010281:Parathyroid Hormone / Q000096:biosynthesis / Q000235:genetics*; D014176:Protein Biosynthesis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis; D014158:Transcription, Genetic
chemical_list: D004275:DNA, Ribosomal; D010281:Parathyroid Hormone; D012336:RNA, Ribosomal, 16S; D011994:Recombinant Proteins