Cytogenetic activities of tobacco particulate matter (TPM) derived from a low to middle tar British cigarette.
Tobacco particulate matter (TPM) derived from an experimental low to middle tar cigarette was tested for its cytogenetic activity upon a low passage number Chinese hamster pulmonary cell line. Examination of the mitotic profiles (after one cell cycle) revealed no interference by the agent with mitotic spindle formation and/or function. However, complete chromosomes (or parts of them) were seen to dislocate from the mitotic spindles. Such an event was probably the result of the chromosome aberrations, substantial numbers of which were observed in second division cells, or through a process of centromere inactivation. In second division cells there was a reduction in the number of diploid cells accompanied by an increase in both hypodiploidy and polyploidy and there was also a non-dose-related increase in endoreduplication. The results demonstrate that TPM was capable of inducing both structural and numerical chromosome aberrations in cultured mammalian cells.
D000818:Animals; D002460:Cell Line; D002869:Chromosome Aberrations; D006224:Cricetinae; D003412:Cricetulus; D008938:Mitosis / Q000187:drug effects*; D010947:Plants, Toxic; D012907:Smoking / Q000009:adverse effects*; D008941:Spindle Apparatus / Q000187:drug effects; D013638:Tars / Q000009:adverse effects*; D014026:Nicotiana
D013638:Tars