Simultaneous retrieval of the heart and liver from a single donor: an evaluation through preservation and transplantation.
The simple, safe, and feasible procurement technique for the heart and liver with no warm ischemic time is reported. Fifteen mongrel dogs were used to form two recipients and one donor combination in each experiment. A midline incision is extended from the suprasternal notch to the pubis, and a catheter is advanced into the aortic root by means of the brachiocephalic artery for monitoring systemic arterial pressure and later for coronary vascular washout with a cold cardioplegic solution. Liver mobilization is carried out first when the core temperature of the liver reaches 27 degrees C, obtained with ice slush in the abdominal cavity. As the core temperature of the liver reaches 20 degrees to 22 degrees C, the aorta and inferior vena cava are clamped just above the diaphragm. After excision of the liver a second team harvests the heart while it continues to beat. The heart and liver were transplanted orthotopically after simple preservation into a cold solution for 12 and 6 hours, respectively. The maximum survival time was 7 hours in heart transplantation and 16 days in liver transplantation. Our method is a simple, safe, feasible technique for acquiring the heart and liver or other visceral organs for transplantation and may have broad clinical application.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D004285:Dogs; D006085:Graft Survival / Q000187:drug effects; D016027:Heart Transplantation; D006982:Hypertonic Solutions / Q000008:administration & dosage; D007036:Hypothermia, Induced / Q000379:methods; D008099:Liver / Q000378:metabolism; D016031:Liver Transplantation; D009200:Myocardial Contraction; D009206:Myocardium / Q000378:metabolism; D009926:Organ Preservation / Q000379:methods*
C024102:Collins' solution; D006982:Hypertonic Solutions; D000255:Adenosine Triphosphate