The type of human papillomavirus present in cervical infections can be determined by the occurrence of specific marker proteins.
Four different proteins have been identified on high resolution two dimensional gels of [35S] methionine labelled human cervical biopsies whose expression correlates with the presence of papillomavirus. They are all basic proteins having molecular weights in the region of 48 to 50 kd and are normally expressed individually in different lesions unless the lesion results from a co-infection of two virus types. Comparison of the occurrence of these marker proteins with the actual HPV type present, determined by in situ filter hybridisation, has shown that two are found exclusively with HPV types 6/11 while the other two are found with types 16/18.
D000956:Antigens, Viral / Q000032:analysis*; D002584:Cervix Uteri / Q000382:microbiology; D004279:DNA, Viral / Q000302:isolation & purification; D005260:Female; D006801:Humans; D027383:Papillomaviridae / Q000235:genetics* / Q000302:isolation & purification; D014412:Tumor Virus Infections / Q000382:microbiology*; D002583:Uterine Cervical Neoplasms / Q000382:microbiology*; D014764:Viral Proteins / Q000032:analysis*
D000956:Antigens, Viral; D004279:DNA, Viral; D014764:Viral Proteins