title: Functional analysis of the marginating pool of human polymorphonuclear leukocytes.
abstract: The intravascular pool of human polymorphonuclear leukocytes (PMN) is composed of one compartment which is circulating and another that is marginated to the vascular endothelium. Administration of B-adrenergic agonists leads to a rapid demargination with an increase in the circulating PMN pool. The marginating PMN has previously been stated to represent an older PMN based on a higher cytochemical alkaline phosphatase activity. With the understanding that circulating PMN are heterogeneous with respect to function and size we undertook the present study to evaluate the contribution of the marginating PMN to functional and volume-dependent heterogeneity. We found that PMN isolated 7 min after epinephrine administration, presumably enriched by marginating PMN, were not different in volume, biochemically measured alkaline phosphatase activity, stimulated superoxide anion release, degranulation, or phagocytosis. These data suggest that the circulating and marginating pools of PMN are interchangeable and that the marginating PMN are not enriched by a particular subpopulation of PMN.
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D002633:Chemotaxis; D003571:Cytochalasin B / Q000494:pharmacology; D004837:Epinephrine / Q000494:pharmacology; D006801:Humans; D009113:Muramidase / Q000378:metabolism; D009504:Neutrophils / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism / Q000502:physiology*; D010084:Oxidation-Reduction; D009195:Peroxidase / Q000378:metabolism; D010587:Phagocytosis
chemical_list: D003571:Cytochalasin B; D009195:Peroxidase; D000469:Alkaline Phosphatase; D009113:Muramidase; D004837:Epinephrine