Effects of gossypol acetic acid on rat luteal cells in vitro.
The action of gossypol acetic acid (GAA) on 125I-hCG binding, gonadotropin-stimulated cAMP accumulation and progesterone production was investigated utilizing rat ovaries. Incubation of luteal cells for 3 h with increasing concentration of GAA caused a significant inhibition of gonadotropin-stimulated steroidogenesis. The inhibitory effect of GAA was concentration dependent. GAA at concentrations of 10-30 micrograms/ml reduced cAMP formation in response to hCG. It was shown that the activity of adenylate cyclase of luteal cells was inhibited by 10 micrograms/ml GAA. GAA at a concentration of 30 micrograms/ml was found to have an inhibitory effect on 8Br-cAMP-stimulated progesterone production. GAA did not affect 125I-hCG binding to LH receptor on the luteal cell surface. These results suggest that in luteal cells GAA inhibits steroidogenesis at the step of gonadotropin-stimulated cAMP formation. Adenylate cyclase of luteal cells was inhibited.
D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000037:antagonists & inhibitors; D000067956:Adenylyl Cyclase Inhibitors; D000818:Animals; D006063:Chorionic Gonadotropin / Q000378:metabolism; D003338:Corpus Luteum / Q000187:drug effects* / Q000378:metabolism; D000242:Cyclic AMP / Q000096:biosynthesis; D005260:Female; D006072:Gossypol / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000633:toxicity; D066298:In Vitro Techniques; D007247:Infertility, Female / Q000139:chemically induced; D011374:Progesterone / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D000067956:Adenylyl Cyclase Inhibitors; D006063:Chorionic Gonadotropin; D015124:8-Bromo Cyclic Adenosine Monophosphate; D011374:Progesterone; D000242:Cyclic AMP; D006072:Gossypol; C028178:gossypol acetic acid