Efficient transformation of phytopathogenic strains of Xanthomonas species.
Efficient transformation and conjugation systems for use in phytopathogenic strains of Xanthomonas species were developed with a dual-function plasmid vector, pBXC12, which was constructed from a newly isolated Xanthomonas citri plasmid, pXCL6, and pBR328. By using this system, pBR328 could also transform the same strains of Xanthomonas. The systems make useful cloning vectors for the study of genes involved in the plant pathogenesis of this species.
D003001:Cloning, Molecular; D003227:Conjugation, Genetic; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000032:analysis; D004587:Electrophoresis, Agar Gel; D005798:Genes, Bacterial; D005822:Genetic Vectors; D009693:Nucleic Acid Hybridization; D010944:Plants / Q000382:microbiology; D010957:Plasmids; D014169:Transformation, Bacterial; D014974:Xanthomonas / Q000235:genetics*
D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes