title: C lambda 2 and C lambda 4 immunoglobulin light chain genes in a wild-derived inbred mouse strain.
abstract: A genomic clone containing the lambda constant (C) region genes (C lambda 2S and C lambda 4S) has been isolated from a genomic library from the mouse strain SPE. SPE is an inbred strain derived from progenitors trapped near Grenada in Spain and has been classified as mus 3 or mus spretus. The sequence of the C lambda 2S gene is virtually identical to that of BALB/c both in the coding region and in flanking sequences, suggesting that it is an expressed gene in the SPE strain. By contrast, the C lambda 4S gene on the same cluster has diverged in sequence from that of BALB/c and contains a large deletion that precludes its normal expression. Whereas BALB/c J lambda 4 region contains substitutions that probably preclude its usage, the SPE J lambda 4 gene includes all sequences required for a functional J gene. Comparison of the C lambda 2S and C lambda 4S gene sequences with those available for BALB/c C lambda 3 and C lambda 1 confirms the close relationship between the C lambda 1-C lambda 4 and C lambda 2-C lambda 3 gene pairs. The C lambda 3 gene of BALB/c is more closely related to C lambda 2S than is C lambda 1 of BALB/c to C lambda 4S. If it is assumed that C lambda 1 and C lambda 2 are respective duplicates of C lambda 4 and C lambda 3 and that these duplications occurred at the same time, then the C lambda 2 gene has been under stronger selective pressure than C lambda 4.
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000835:Animals, Wild / Q000235:genetics* / Q000276:immunology; D001483:Base Sequence; D002874:Chromosome Mapping; D004262:DNA Restriction Enzymes; D007127:Immunoglobulin Constant Regions / Q000235:genetics*; D007146:Immunoglobulin lambda-Chains / Q000235:genetics*; D007136:Immunoglobulins / Q000235:genetics*; D051379:Mice; D008815:Mice, Inbred Strains / Q000235:genetics* / Q000276:immunology; D009115:Muridae / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
chemical_list: D007127:Immunoglobulin Constant Regions; D007146:Immunoglobulin lambda-Chains; D007136:Immunoglobulins; D004262:DNA Restriction Enzymes