title: Characterization of Triatoma virus, a picorna-like virus isolated from the triatomine bug Triatoma infestans.
abstract: Some properties of Triatoma virus (TrV), a picorna-like virus recently isolated from Triatoma infestans, have been studied. Electron microscopic observations of purified viral preparations showed the presence of non-enveloped viral particles 30 nm in diameter. The sedimentation coefficient of virus particles was about 165S and the buoyant density in CsCl was 1.39 g/ml. The viral genome was composed of one single-stranded RNA molecule with an Mr of 3 x 10(6). Three major polypeptides with Mr values of 39K, 37K and 33K and a minor one of about 45K were found in the virus particle. TrV particles contain about 35% RNA and 65% protein by weight. These data support the classification of this virus in the family Picornaviridae.
mesh_terms: D000818:Animals; D008854:Microscopy, Electron; D008970:Molecular Weight; D010849:Picornaviridae / Q000302:isolation & purification* / Q000648:ultrastructure; D012367:RNA, Viral / Q000032:analysis; D014224:Triatoma / Q000382:microbiology*; D014225:Triatominae / Q000382:microbiology*; D014461:Ultracentrifugation; D014764:Viral Proteins / Q000032:analysis
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins