Evaluation of nuclear magnetic resonance spectroscopy for determination of deuterium abundance in body fluids: application to measurement of total-body water in human infants.
Nuclear magnetic resonance (NMR) spectroscopy was used to quantitate abundance of 2H in body water of human infants. This method provides precise measurement of total-body water without the extensive sample preparation requirements of previously described methods for determination of 2H content in body fluids. 2H2O (1 g/kg body weight) was administered to infants and saliva and urine were collected for up to 5 h. An internal standard was added directly to the fluid specimen and 2H enrichment in water was measured by NMR spectroscopy. Working range of deuterium abundance was 0.04-0.32 atom %. Coefficients of variation for saliva samples at 0.20 atom % 2H was 1.97%. 2H content in urine and saliva water reached a plateau by 4 h after administration, and amounts in the two fluids were virtually identical. Mean total-body water determination for six infants was 58.3 +/- 5.8% of body weight (range 53-66%).
D001826:Body Fluids / Q000032:analysis*; D001834:Body Water / Q000032:analysis*; D003903:Deuterium / Q000032:analysis*; D017666:Deuterium Oxide; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D007201:Indicator Dilution Techniques; D007223:Infant; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D012463:Saliva / Q000032:analysis; D014867:Water / Q000032:analysis
D014867:Water; D003903:Deuterium; D017666:Deuterium Oxide