title: Prostaglandin E2 directly protects isolated rat gastric surface cell membranes against bile salts.
abstract: Rat gastric surface cell membranes were prepared and the effect of taurocholic acid assessed by ESR spectroscopy using the 16-doxylstearic acid spin label. Taurocholic acid increased the polar part of the spectra, indicating an augmented amount of spin label molecules with a polar environment. Concomitantly, mobility of the spin label molecule was augmented. The effect of taurocholic acid was completely prevented by the previous addition of prostaglandin E2. This suggests a direct protective efficiency of prostaglandin E2 on rat gastric surface cell membranes without the metabolic participation of intact cells.
mesh_terms: D000818:Animals; D001647:Bile Acids and Salts / Q000633:toxicity*; D002462:Cell Membrane / Q000187:drug effects; D015232:Dinoprostone; D004578:Electron Spin Resonance Spectroscopy; D005260:Female; D005753:Gastric Mucosa / Q000187:drug effects*; D066298:In Vitro Techniques; D011458:Prostaglandins E / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D001647:Bile Acids and Salts; D011458:Prostaglandins E; D015232:Dinoprostone