Synthesis of decadeoxyribonucleotides containing N6-methyladenine, N4-methylcytosine, and 5-methylcytosine: recognition and cleavage by restriction endonucleases (nucleosides and nucleotides part 74).
The naturally-occurring modified bases, N6-methyladenine, N4-methylcytosine, and 5-methylcytosine were chemically introduced in place of the adenine or cytosine in the decadeoxyribonucleotides containing recognition sequences of Bgl II, Sau 3AI, Mbo I and Mfl I. The modified oligomers bind to the enzymes but the rates of cleavage by the enzymes are variable.
D044503:5-Methylcytosine; D000225:Adenine / Q000031:analogs & derivatives*; D002942:Circular Dichroism; D003596:Cytosine / Q000031:analogs & derivatives*; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D007202:Indicators and Reagents; D007700:Kinetics; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis*; D013379:Substrate Specificity
D044503:5-Methylcytosine; D000225:Adenine; D003596:Cytosine; D004262:DNA Restriction Enzymes; D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides; C039052:N(4)-methylcytosine; C000730567:6-methyladenine