Inhibition of cAMP-phosphodiesterase by molybdate.
Inhibition of 3':5'-cyclic-AMP-5'-nucleotidohydrolase (EC 3.1.4.17) type II (cAMP-phosphodiesterase) by sodium molybdate was studied: While determination of inorganic phosphate and 5'-ribonucleotide phosphohydrolase (5'-nucleotidase) (EC 3.1.3.5) activity was not disturbed by sodium molybdate in concentrations up to 10 mM, cAMP-phosphodiesterase was inhibited by millimolar concentrations of molybdate. The half maximal effect was observed at about 2 mM sodium molybdate (0.75 mM cAMP in the assay).
D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000037:antagonists & inhibitors*; D015720:5'-Nucleotidase; D007202:Indicators and Reagents; D007700:Kinetics; D008982:Molybdenum / Q000494:pharmacology*; D009708:Nucleotidases / Q000378:metabolism
D007202:Indicators and Reagents; C044659:molybdate; D008982:Molybdenum; D009708:Nucleotidases; D015720:5'-Nucleotidase; D015105:3',5'-Cyclic-AMP Phosphodiesterases