Parallelism between superoxide production of peritoneal exudate cells and lung granulomatous response in mice vaccinated with BCG cell walls.
Since peritoneal macrophages are reported to be different from alveolar macrophages in their activated states, we examined whether O-2 production, one of the parameters of macrophage activation, in mouse peritoneal exudate cells (PEC) is enhanced under the condition in which lung granuloma, the accumulation of activated macrophages, is produced with Bacillus Calmette-Guérin (BCG) cell wall (CW). As a result, we observed the enhanced O-2 production of PEC that occurs in parallel with lung granuloma formation; high responders, C56BL/6 mice, showed high O-2 production of PEC whereas low responders, C3H/He and DBA/1 mice showed low O-2 production of PEC, suggesting that enhanced O-2 production of PEC as well as lung granuloma formation is genetically controlled. Results from T cell-depleted mice and allogeneic bone marrow chimeric mice also showed the occurrence of this parallelism. From these findings, we presumed that circulating macrophage activating factor and other lymphokines produced by BCG CW-sensitized T cells may activate both peritoneal macrophages and lung macrophages.
D000818:Animals; D001500:BCG Vaccine / Q000008:administration & dosage* / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000008:administration & dosage; D002678:Chimera; D005260:Female; D006099:Granuloma / Q000175:diagnosis* / Q000276:immunology; D008168:Lung / Q000166:cytology; D008212:Lymphocyte Depletion; D008262:Macrophage Activation; D008264:Macrophages / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D008809:Mice, Inbred C3H / Q000145:classification; D008810:Mice, Inbred C57BL / Q000145:classification; D010529:Peritoneal Cavity / Q000166:cytology*; D013481:Superoxides / Q000096:biosynthesis*; D013601:T-Lymphocytes / Q000276:immunology
D001500:BCG Vaccine; D001425:Bacterial Outer Membrane Proteins; D013481:Superoxides