Effects of release factor context at UAA codons in Escherichia coli.
In Escherichia coli, nonsense suppression at UAA codons is governed by the competition between a suppressor tRNA and the translational release factors RF1 and RF2. We have employed plasmids carrying the genes for RF1 and RF2 to measure release factor preference at UAA codons at 13 different sites in the lacI gene. We show here that the activity of RF1 and RF2 varies according to messenger context. RF1 is favored at UAA codons which are efficiently suppressed. RF2 is preferred at poorly suppressed sites.
D001426:Bacterial Proteins / Q000502:physiology*; D001483:Base Sequence; D001667:Binding, Competitive; D003062:Codon; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D010443:Peptide Chain Termination, Translational; D010454:Peptide Termination Factors / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics*; D013329:Structure-Activity Relationship; D013489:Suppression, Genetic
D001426:Bacterial Proteins; D003062:Codon; D029968:Escherichia coli Proteins; D010454:Peptide Termination Factors; D012333:RNA, Messenger; C041460:peptide chain termination release factor 2; C489005:prfA protein, E coli; C489006:prfB protein, E coli