title: Expression of tumor necrosis factor in vitro by human mononuclear phagocytes stimulated with whole Mycobacterium bovis BCG and mycobacterial antigens.
abstract: Four mycobacterial preparations directly stimulated human blood monocytes and alveolar macrophages to produce tumor necrosis factor (TNF). Monoclonal antibody against TNF blocked (99%) TNF activity. Lipopolysaccharide was not responsible for the TNF activity generated; activity was unaffected in the presence of polymyxin B.
mesh_terms: D000942:Antigens, Bacterial / Q000276:immunology*; D001992:Bronchoalveolar Lavage Fluid; D006801:Humans; D066298:In Vitro Techniques; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D008050:Lipid A / Q000037:antagonists & inhibitors; D008262:Macrophage Activation; D008264:Macrophages / Q000276:immunology*; D009163:Mycobacterium bovis / Q000276:immunology*; D011112:Polymyxin B / Q000494:pharmacology; D013997:Time Factors; D014373:Tuberculin / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
chemical_list: D000942:Antigens, Bacterial; D008050:Lipid A; D014373:Tuberculin; D014409:Tumor Necrosis Factor-alpha; D011112:Polymyxin B