Use of hybridot assay to screen for BK and JC polyomaviruses in non-immunosuppressed patients.
Urine samples from 50 patients attending a genitourinary outpatient clinic and from 13 renal allograft recipients were investigated for evidence of infection with human BK and JC polyomaviruses using cytology and a new DNA hybridot assay. Forty four per cent of samples from the renal allograft recipients were positive by cytology and 75% by DNA hybridisation, indicating that hybridot assay is more sensitive than cytological screening. BK and JC viral DNA was found in 20% of the patients attending the genitourinary clinic, showing infection with BK virus and JC virus in a group of patients with clinical conditions not normally associated with immunological deficiency-a finding that has not been reported before.
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001739:BK Virus / Q000302:isolation & purification*; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D006801:Humans; D007108:Immune Tolerance; D007577:JC Virus / Q000302:isolation & purification*; D016030:Kidney Transplantation; D008297:Male; D008722:Methods; D008875:Middle Aged; D009693:Nucleic Acid Hybridization; D011120:Polyomavirus / Q000302:isolation & purification*; D014556:Urine / Q000382:microbiology
D004279:DNA, Viral