Macromolecular synthesis at the early stage of herpes simplex virus type 2 (HSV-2) latency in a human neuroblastoma cell line IMR-32: repression of late viral polypeptide synthesis and accumulation of cellular heat-shock proteins.
We have shown that a latent infection of herpes simplex virus type 2 (HSV-2) can be established in a human neuroblastoma cell line IMR-32 if the infected cells are cultured at 40 degrees C. In the present study, viral polypeptides and cellular heat-shock proteins which were synthesized in HSV-2 infected IMR-32 cells cultured at 40 degrees C were analyzed by polyacrylamide gel electrophoresis. It was found that the synthesis of late viral polypeptide ICP 5 was markedly reduced in the infected cells at 40 degrees C as compared with those at 37 degrees C. Although infection of IMR-32 cells with HSV-2 at 40 degrees C resulted in shutoff of cellular protein synthesis, it was found that some cellular heat-shock proteins (90, 72 and 70 kd polypeptides) were synthesized and accumulated intracellularly. These findings suggest that modification of cascade regulation of HSV-2 polypeptide synthesis and/or accumulation of heat-shock proteins may be involved in the incomplete arrest of virus growth and in survival of the infected cells, leading to the establishment of HSV-2 latency in IMR-32 cells.
D002460:Cell Line; D006360:Heat-Shock Proteins / Q000096:biosynthesis*; D006801:Humans; D007700:Kinetics; D009447:Neuroblastoma / Q000382:microbiology; D014176:Protein Biosynthesis; D011506:Proteins / Q000032:analysis; D018139:Simplexvirus / Q000254:growth & development / Q000378:metabolism*; D013696:Temperature; D014764:Viral Proteins / Q000032:analysis / Q000096:biosynthesis*
D006360:Heat-Shock Proteins; D011506:Proteins; D014764:Viral Proteins