The influence of the dT.dG mispair on the activity of the human DNA(cytosine-5)methyltransferase.
Synthetic oligodeoxynucleotides containing a dT.dG mispair at a centrally located d(pCG) dimer are methylated at a moderate rate by highly purified human DNA(cytosine-5)methyltransferase (E.C. 2.1.1.37). The presence of a mispaired dT in one strand induced the enzyme to preferentially methylate the opposite strand.
D001483:Base Sequence; D004247:DNA / Q000378:metabolism*; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000378:metabolism*; D004262:DNA Restriction Enzymes / Q000378:metabolism; D003849:Deoxyguanosine; D006801:Humans; D008745:Methylation; D010920:Placenta / Q000201:enzymology; D013329:Structure-Activity Relationship; D013379:Substrate Specificity; D013936:Thymidine
D004247:DNA; D004248:DNA (Cytosine-5-)-Methyltransferases; D004262:DNA Restriction Enzymes; D003849:Deoxyguanosine; D013936:Thymidine