title: Identification of the promoter of the Bacillus subtilis sdh operon.
abstract: The Bacillus subtilis sdhCAB operon contains the structural genes for the three subunits of the membrane bound succinate dehydrogenase complex. An sdh-specific transcript of about 3,450 nucleotides was detected in vegetative bacteria. S1 nuclease mapping experiments showed that the sdh operon is transcribed from a sigma-43 promoter; the transcript starts at a guanosine residue 90 base pairs upstream from the first gene of the operon, sdhC. No sdh transcript was found in B. subtilis carrying the sdh-115 mutation, which decreases expression of the sdh operon by more than 99%. The sdh-115 mutation is a G-to-A transition in the -35 region of the sigma-43 promoter. The sdh operon is sensitive to glucose repression. When the sdh promoter region was used to drive transcription of the cat-86 gene this gene also became glucose repressed.
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics*; D002874:Chromosome Mapping; D004262:DNA Restriction Enzymes; D005786:Gene Expression Regulation; D005798:Genes, Bacterial; D005947:Glucose / Q000502:physiology; D009876:Operon; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics; D012808:Sigma Factor / Q000235:genetics; D013385:Succinate Dehydrogenase / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D012333:RNA, Messenger; D012808:Sigma Factor; D013385:Succinate Dehydrogenase; D004262:DNA Restriction Enzymes; D005947:Glucose