title: Escherichia coli rep gene: sequence of the gene, the encoded helicase, and its homology with uvrD.
abstract: The sequence of a 2.67-kilobase section of the Escherichia coli chromosome that contains the rep gene has been determined. This gene codes for a protein of predicted Mr 72,800, a DNA helicase, which is also a single-stranded DNA-dependent ATPase. The sequenced region contains an open reading frame of the correct length and orientation to encode the Rep protein. A secondary structure for the protein can be formulated from the amino acid sequence. We have compared both the primary and the secondary structures of Rep with other proteins and find the greatest homology between Rep and E. coli helicase II, the product of the uvrD gene.
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D004265:DNA Helicases; D004262:DNA Restriction Enzymes; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D029968:Escherichia coli Proteins; D005796:Genes; D005798:Genes, Bacterial; D008970:Molecular Weight; D012689:Sequence Homology, Nucleic Acid
chemical_list: D029968:Escherichia coli Proteins; C506744:rep protein, E coli; D004262:DNA Restriction Enzymes; D000251:Adenosine Triphosphatases; C506746:UvrD protein, E coli; D004265:DNA Helicases