Cloning and sequencing of the Escherichia coli chlEN operon involved in molybdopterin biosynthesis.
The nucleotide sequence of a HinPI-HpaII restriction nuclease fragment which complemented a delta chlE strain of Escherichia coli was determined. Two open reading frames were deduced to be the structural genes for ChlE and ChlN proteins, which have molecular weights of 44,067 and 26,719, respectively. Both proteins were required for complementing a chromosomal deletion of the chlE locus. The chlE and chlN genes were transcribed from a common promoter, chlEp, located upstream of chlE. Transcriptional and translational signal sequences were recognized in this region.
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000032:analysis; D001483:Base Sequence; D003001:Cloning, Molecular; D003067:Coenzymes / Q000096:biosynthesis*; D004269:DNA, Bacterial / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D008667:Metalloproteins / Q000096:biosynthesis*; D008969:Molecular Sequence Data; D008982:Molybdenum / Q000378:metabolism*; D000090006:Molybdenum Cofactors; D009876:Operon; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011621:Pteridines / Q000096:biosynthesis*
D001426:Bacterial Proteins; D003067:Coenzymes; D004269:DNA, Bacterial; D008667:Metalloproteins; D008982:Molybdenum; D000090006:Molybdenum Cofactors; D011621:Pteridines; C022261:molybdopterin