Retrovirus integration and chromatin structure: Moloney murine leukemia proviral integration sites map near DNase I-hypersensitive sites.
The chromatin conformation of mouse genome regions containing Moloney murine leukemia proviral intergration sites in two Mov mouse strains and randomly selected integration sites in virus-infected mouse 3T3 fibroblasts was analyzed. All integrations have occurred into chromosomal regions containing several DNase-hypersensitive sites, and invariably the proviral integration sites map within a few hundred base pairs of a DNase-hypersensitive site. The probability that this close association between proviral integration sites and DNase-hypersensitive sites was due to chance was calculated to be extremely low (2 X 10(-4]. Because the proviral integrations analyzed were not selected for an altered phenotype, our results suggest that DNase-hypersensitive regions are preferred targets for retrovirus integration.
D000818:Animals; D002478:Cells, Cultured; D002843:Chromatin / Q000032:analysis*; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant / Q000378:metabolism; D003850:Deoxyribonuclease I / Q000378:metabolism*; D005814:Genes, Viral; D051379:Mice; D008979:Moloney murine leukemia virus / Q000235:genetics*; D013379:Substrate Specificity
D002843:Chromatin; D004274:DNA, Recombinant; D004262:DNA Restriction Enzymes; D003850:Deoxyribonuclease I