Cloning of the beta subunit of the leukocyte adhesion proteins: homology to an extracellular matrix receptor defines a novel supergene family.
We have isolated cDNA clones encoding the beta subunit of the human LFA-1, Mac-1, and p150,95 family of leukocyte adhesion proteins. The deduced 769-amino-acid sequence defines a cysteine-rich polypeptide with the characteristic features of an integral membrane protein. Peptide sequence data, Northern blot analysis, and Southern blot analysis suggest that a single gene encodes the beta subunit of all three leukocyte adhesion proteins. There is 45% homology between the beta subunit sequence and band III of integrin, a chick fibronectin and laminin receptor. This homology defines a new supergene family of cellular adhesion proteins.
D000595:Amino Acid Sequence; D000954:Antigens, Surface / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification; D005109:Extracellular Matrix / Q000378:metabolism*; D006801:Humans; D007256:Information Systems; D016023:Integrins; D016169:Lymphocyte Function-Associated Antigen-1; D046911:Macromolecular Substances; D008565:Membrane Proteins / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000235:genetics*
D000954:Antigens, Surface; D016023:Integrins; D016169:Lymphocyte Function-Associated Antigen-1; D046911:Macromolecular Substances; D008565:Membrane Proteins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D004247:DNA