A comparative study on proteins released from metaphase chromosomes after digestion with restriction endonucleases and deoxyribonuclease I.
Extensive digestion of Chinese hamster metaphase chromosomes with Alu I, Hae III and Hinf I released up to 40 distinct chromosomal proteins. Some of the proteins released by Hae III or Hinf I were enriched in the protein moiety liberated by Alu I but several proteins released by Hae III were not released by Alu I digestion. The amount of chromosomal protein released by deoxyribonuclease I (DNase I) was comparable to that liberated by the three restriction enzymes so far tested, while only four abundant protein species were detectable in the protein moiety released by DNase I. Two of them with molecular weights of 58,000 and 50,000 were also released by the three restriction enzymes and are similar in size to those found previously in the core-like structure of histone-depleted chromosomes.
D000818:Animals; D002460:Cell Line; D002875:Chromosomes / Q000032:analysis*; D006224:Cricetinae; D003412:Cricetulus; D004262:DNA Restriction Enzymes; D003850:Deoxyribonuclease I; D003852:Deoxyribonucleoproteins / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D008677:Metaphase; D008970:Molecular Weight
D003852:Deoxyribonucleoproteins; D004262:DNA Restriction Enzymes; D003850:Deoxyribonuclease I