Simultaneous light and electron microscopic observation of immunolabeled liver 27 KD gap junction protein on ultra-thin cryosections.
We report on immunolabeling of gap junction protein in rat liver. Simultaneous light and electron microscopic immunolabeling of ultra-thin frozen sections was performed to confirm that the antigenic targets of polyclonal antibodies and a monoclonal 27 KD antibody (12/1 C5) are the gap junctions. Our results clearly demonstrate that the immunoreactive sites determined by indirect immunofluorescence correspond to immunogold-labeled gap junctions identified in the same section according to electron microscopic criteria. Our results also support the concept that the 27 KD protein is a major constituent of gap junctions.
D000818:Animals; D000911:Antibodies, Monoclonal; D017630:Connexins; D005455:Fluorescent Antibody Technique; D005629:Frozen Sections; D006046:Gold; D006651:Histocytochemistry; D007159:Immunologic Tests; D007365:Intercellular Junctions / Q000032:analysis*; D008099:Liver / Q000032:analysis* / Q000648:ultrastructure; D008565:Membrane Proteins / Q000032:analysis*; D008854:Microscopy, Electron; D051381:Rats
D000911:Antibodies, Monoclonal; D017630:Connexins; D008565:Membrane Proteins; D006046:Gold