The cholecalciferol sulphate system in mammals.
7-Dehydrocholesterol sulphate has been identified in human and rat skin. The compound was isolated by anion exchange chromatography and following hydrolysis it was characterized by high-performance liquid chromatography and gas chromatography-mass spectrometry. Experiments with rats showed that 7-dehydrocholesterol sulphate can serve as a precursor of cholecalciferol sulphate and 25-hydroxy-cholecalciferol 3-sulphate, the latter compound being present in significant amounts in human blood. The sulphated sterols identified represent a previously unknown secosteroid system in mammals.
D000818:Animals; D002112:Calcifediol / Q000031:analogs & derivatives / Q000378:metabolism; D002762:Cholecalciferol / Q000378:metabolism* / Q000031:analogs & derivatives; D002775:Cholestadienols / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D003684:Dehydrocholesterols / Q000032:analysis* / Q000378:metabolism; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D051381:Rats; D011919:Rats, Inbred Strains; D012867:Skin / Q000032:analysis*
D002775:Cholestadienols; D003684:Dehydrocholesterols; C026415:vitamin D3 sulfoconjugate; C052375:7-dehydrocholesterol-3-sulfate ester; D002762:Cholecalciferol; C046949:25-hydroxyvitamin D3 3-sulfate ester; D002112:Calcifediol