Studies on the inhibition of brain synaptosomal Na+/K+-ATPase by mercury chloride and methyl mercury chloride.
The effect of mercury chloride (HgCl2) and methyl mercury chloride (MeHg) on brain synaptosomal Na+/K+-ATPase was determined in vitro. It was shown that HgCl2 is a more powerful inhibitor than MeHg. The IC50 were 5 X 10(-7) M for HgCl2 and 2.3 X 10(-6) M for MeHg. A non-competitive type of inhibition was observed with the two mercurials. Removal of contaminating lipids with the non-ionic detergent Lubrol did not affect the inhibition of either mercurial. It is concluded that non-essential lipids do not play a significant role in the inhibitory effect of MeHg and that the potency of these mercurials on to inhibit brain synaptosomal Na+/K+-ATPase largely depends on their capacity to block sulfhydryl groups.
D000818:Animals; D001921:Brain / Q000201:enzymology*; D066298:In Vitro Techniques; D008297:Male; D008627:Mercuric Chloride / Q000633:toxicity*; D008767:Methylmercury Compounds / Q000633:toxicity*; D051381:Rats; D011919:Rats, Inbred Strains; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors*; D013574:Synaptosomes / Q000201:enzymology
D008767:Methylmercury Compounds; D008627:Mercuric Chloride; D000254:Sodium-Potassium-Exchanging ATPase; C004925:methylmercuric chloride