6-beta-Iodopenicillanate as a probe for the classification of beta-lactamases.
An inactivator of serine beta-lactamases, 6 beta-iodopenicillanate, can be utilized as a probe in the classification of beta-lactamases. It is a substrate for class-B Zn2+-containing beta-lactamase II. Although it inactivates enzymes from both classes A and C, it is much more efficient for the former group, with which it sometimes interacts following a branched pathway. On the basis of these observations, predictions are made concerning the class to which several enzymes belong.
D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010397:Penicillanic Acid / Q000494:pharmacology* / Q000031:analogs & derivatives; D013053:Spectrophotometry; D013843:Thiazines; D065093:beta-Lactamase Inhibitors; D001618:beta-Lactamases / Q000145:classification*
D013843:Thiazines; D065093:beta-Lactamase Inhibitors; C051511:2,3-dihydro-2,2-dimethyl-1,4-thiazine-3,6-dicarboxylate; D010397:Penicillanic Acid; C031528:6-iodopenicillanic acid; D001618:beta-Lactamases