[Preservation of the beta-galactosidase activity in E. coli cells containing the recombinant pUC19 plasmid].
Two BglII fragments of pJC703 cosmid were inserted into the plasmid PUC19. E. coli cells containing the recombinant PUC19/A plasmid acquired rifampicin resistance due to inserted rpoB gene but still expressed the Lac+ phenotype.
D002874:Chromosome Mapping; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000235:genetics*; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005696:Galactosidases / Q000235:genetics*; D010957:Plasmids; D001616:beta-Galactosidase / Q000235:genetics*
D004269:DNA, Bacterial; D004274:DNA, Recombinant; D004262:DNA Restriction Enzymes; D005696:Galactosidases; D001616:beta-Galactosidase
Sokhranenie beta-galaktozidaznoĭ aktivnosti kletkami E. coli, soderzhashchimi rekombinantnuiu plazmidu pUC19.