Activated c-Ha-ras oncogene with a guanine to thymine transversion at the twelfth codon in a human stomach cancer cell line.
The rat fibroblast cell line Rat 1 was transfected with total DNA of a gastrocarcinoma cell line, BGC-823. The transforming gene was cloned from the genomic library of the secondary transformants using in situ hybridization with a probe of the human Alu repeat sequence. This cloned gene is homologous to the protooncogene c-Ha-ras. The activation lesion of the transforming gene was identified by sequence analysis as a single nucleotide substitution of thymine for guanine in the 12th codon. This results in the substitution of valine for glycine at the 12th amino acid of the Mr 21,000 protein.
D000230:Adenocarcinoma / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D003062:Codon; D004262:DNA Restriction Enzymes / Q000378:metabolism; D015245:Deoxyribonuclease BamHI; D006147:Guanine / Q000032:analysis*; D008970:Molecular Weight; D009857:Oncogenes; D010455:Peptides / Q000235:genetics; D010957:Plasmids; D012333:RNA, Messenger; D051381:Rats; D013274:Stomach Neoplasms / Q000235:genetics*; D013941:Thymine / Q000032:analysis*; D015291:Transforming Growth Factors
D003062:Codon; D010455:Peptides; D012333:RNA, Messenger; D006147:Guanine; D015291:Transforming Growth Factors; D004262:DNA Restriction Enzymes; D015245:Deoxyribonuclease BamHI; D013941:Thymine