Two enzyme immunoassays for the detection of antibody to rodent coronaviruses.
Two enzyme immunoassays for detection of antibody to rodent coronaviruses were compared. Mouse hepatitis virus (MHV), strain JHM, antigen was in the form of formalin-fixed, infected 17 C1 1 cells. This antigen detected antibody to the homologous strain of MHV as well as to two heterologous MHV strains and a serologically related rat coronavirus, sialodacryodenititis virus. Antibody titers in assays using horseradish peroxidase (HRP)-conjugated or ureiase-conjugated anti-mouse IgG were substantially higher than in an indirect immunofluorescence assay. The ureiase assay was somewhat more sensitive than the HRP assay. MHV-JHM antigen was stable under a variety of storage conditions for at least two months.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D000956:Antigens, Viral / Q000276:immunology; D003332:Coronaviridae / Q000276:immunology* / Q000302:isolation & purification; D005455:Fluorescent Antibody Technique; D006735:Horseradish Peroxidase; D007124:Immunoenzyme Techniques; D051379:Mice; D008815:Mice, Inbred Strains; D006517:Murine hepatitis virus / Q000276:immunology; D014510:Urease
D000914:Antibodies, Viral; D000956:Antigens, Viral; D006735:Horseradish Peroxidase; D014510:Urease