An ion-exchange capture technique for routine identification of faecal viruses by electron microscopy.
Faecal specimens from 520 patients with non-bacterial, gastroenteritis were examined by electron microscopy using four methods. These were (1) a direct dip method, (2) low-speed centrifugation, (3) ultracentrifugation and (4) a calcium phosphate method. The calcium phosphate method combined with low-speed centrifugation (750 X g, 2,100 X g) was considered overall best. The calcium phosphate method makes it possible to handle a large number of faecal specimens by saving considerable time and labour.
D000260:Adenoviruses, Human / Q000302:isolation & purification* / Q000648:ultrastructure; D002130:Calcium Phosphates; D002498:Centrifugation; D003332:Coronaviridae / Q000302:isolation & purification* / Q000648:ultrastructure; D005243:Feces / Q000382:microbiology*; D005759:Gastroenteritis / Q000382:microbiology*; D006801:Humans; D008854:Microscopy, Electron; D012401:Rotavirus / Q000302:isolation & purification* / Q000648:ultrastructure; D014461:Ultracentrifugation
D002130:Calcium Phosphates