Total assignments of the 1H NMR of decanucleotide duplexes containing the recognition sites of restriction enzymes.
Complementary DNAs, decanucleotides, were synthesized by solid phase synthesis. They contain six base pairs with different sequences in the center, flanked by GC pairs to stabilize the ends of the helix. All the imino and non-exchangeable protons of these decamers in aqueous solution were assigned by NOESY experiment. Sequence dependent exchange rates of imino protons and the chemical shifts of non-exchangeable protons were examined.
D001482:Base Composition; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides; D013379:Substrate Specificity
D009838:Oligodeoxyribonucleotides; D004262:DNA Restriction Enzymes