Expression of anti-sense mRNA in H-ras transfected NIH/3T3 cells does not suppress the transformed phenotype.
We have attempted to reverse the transformed phenotype of cells expressing the H-ras oncogene. A plasmid in which the first exon of the H-ras oncogene was coupled to the SV40 early promoter in an anti-sense orientation was constructed. This construct was introduced into a clone of H-ras-transformed NIH/3T3 cells. Simultaneous expression of both the SV40 anti-sense construct and H-ras was observed. Anti-sense RNA was present in a 10-20-fold excess over sense H-ras RNA. Only a small fraction of the cytoplasmic RNA was present in a sense: anti-sense duplexed form. The expression of anti-sense H-ras RNA was not accompanied by a phenotypic reversion of transformed cells. The only phenotypic reversion we observed was accompanied by a loss of transfected H-ras sequences. The loss of transfected H-ras sequences occurs with a high frequency in cells supertransfected with the SV40 anti-sense construct.
D000818:Animals; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D005814:Genes, Viral; D051379:Mice; D008815:Mice, Inbred Strains; D009857:Oncogenes; D010641:Phenotype; D012333:RNA, Messenger / Q000235:genetics*; D013539:Simian virus 40 / Q000235:genetics; D014158:Transcription, Genetic; D014162:Transfection
D012333:RNA, Messenger