Analysis of murine cellular receptors for tumor-killing factor.
Receptors for tumor-killing factor (TKF) on the surface of murine cells were analyzed using radioiodinated TKF. Not only sensitive cells but also insensitive cells were found to have specific receptors. Among the sensitive cells, no clear relation was observed between the number of receptors on the cell surface and sensitivity to TKF. Compounds affecting microfilaments (cytochalasin B and D) and microtubules (colchicine and Colcemid) significantly inhibited cytolysis of sensitive cells induced by receptor-bound TKF. It is concluded that internalization of receptor-bound TKF is a prerequisite for triggering cytolysis.
D008841:Actin Cytoskeleton / Q000187:drug effects; D000818:Animals; D002460:Cell Line; D003078:Colchicine / Q000494:pharmacology; D003572:Cytochalasins / Q000494:pharmacology; D003603:Cytotoxins / Q000378:metabolism* / Q000494:pharmacology; D007457:Iodine Radioisotopes; D051379:Mice; D008870:Microtubules / Q000187:drug effects; D011956:Receptors, Cell Surface / Q000032:analysis*; D018124:Receptors, Tumor Necrosis Factor
D003572:Cytochalasins; D003603:Cytotoxins; D007457:Iodine Radioisotopes; D011956:Receptors, Cell Surface; D018124:Receptors, Tumor Necrosis Factor; D003078:Colchicine