Chromosome 8 breakpoint far 3' of the c-myc oncogene in a Burkitt's lymphoma 2;8 variant translocation is equivalent to the murine pvt-1 locus.
The 2;8 variant translocation of human Burkitt's lymphomas is closely related cytogenetically to the t(6;15) of murine plasmacytomas; both involve a reciprocal exchange between the Ig kappa locus and a band region indistinguishable from that bearing the c-myc oncogene. To define their molecular relationship, we have compared cloned chromosome 8 DNA from the t(2;8) breakpoint in the human Burkitt's lymphoma JBL2 with cloned DNA from the murine pvt-1 locus, the major chromosome 15 breakpoint region in murine t(6;15). DNA sequencing and Southern blot analysis shows that these two regions are homologous. Thus the t(2;8) in JBL2 is the molecular equivalent of many murine t(6;15). The murine pvt-1 locus lies an unknown distance 3' of c-myc; analysis of DNA from several tumours with c-myc amplification reveals that pvt-1 is co-amplified in at least one case, placing pvt-1 approximately 100-500 kb 3' of c-myc. The significance of these results with respect to the role of pvt-1 in tumorigenesis is discussed.
D000818:Animals; D001483:Base Sequence; D002051:Burkitt Lymphoma / Q000235:genetics*; D002898:Chromosomes, Human, Pair 8; D004262:DNA Restriction Enzymes; D004251:DNA Transposable Elements; D005784:Gene Amplification; D005814:Genes, Viral; D014644:Genetic Variation; D006801:Humans; D051379:Mice; D009857:Oncogenes; D010954:Plasmacytoma / Q000235:genetics*; D013045:Species Specificity; D014158:Transcription, Genetic; D014178:Translocation, Genetic
D004251:DNA Transposable Elements; D004262:DNA Restriction Enzymes