Protein-DNA interactions in vivo upstream of a cell cycle-regulated human H4 histone gene.
Cell cycle-dependent histone genes are transcribed at a basal level throughout the cell cycle, with a three- to fivefold increase during early S phase. Protein-DNA interactions in the 5' promoter region of a cell cycle-regulated human H4 histone gene have been analyzed at single-nucleotide resolution in vivo. This region contains two sites, with four potential protein-binding domains, at which the DNA is protected from reaction with dimethyl sulfate in cells and from digestion with deoxyribonuclease I in nuclei. These protein-DNA interactions persist during all phases of the cell cycle and dissociate with 0.16 to 0.2M sodium chloride.
D001483:Base Sequence; D002453:Cell Cycle; D002460:Cell Line; D004247:DNA; D004262:DNA Restriction Enzymes; D003850:Deoxyribonuclease I; D005786:Gene Expression Regulation; D006657:Histones / Q000235:genetics*; D006801:Humans; D009693:Nucleic Acid Hybridization; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D013463:Sulfuric Acid Esters
D006657:Histones; D013463:Sulfuric Acid Esters; D004247:DNA; D004262:DNA Restriction Enzymes; D003850:Deoxyribonuclease I; C007482:dimethyl sulfate