[Cloning of DNA fragments of the genetic transfer factor pAP39].
Large HindIII digested fragments of the plasmid pAP39 have been cloned on the cosmid vector pHC79. The study of the structure of HindIII fragments of plasmid pAP39 in the recombinant plasmids has shown that these fragments are represented by f1 + f2 fragments from the plasmid pAP1055, by f1 + f6 fragments from the plasmid pAP1056, by f2 + f3 fragments from the plasmid pAP1057 and by two f3 fragment from the plasmid pAP1058. Physical maps of the recombinant plasmids have been constructed. The plasmid pAP39 is shown to contain two functionally active tra regions.
D003001:Cloning, Molecular; D003360:Cosmids; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D005144:F Factor; D011995:Recombination, Genetic
D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes
Klonirovanie fragmentov DNK faktora geneticheskogo perenosa pAP39.