title: Pseudomonas mutant strains that accumulate androstane and seco-androstane intermediates from bile acids.
abstract: Transposon mutant strains which were affected in bile acid catabolism were isolated from four Pseudomonas spp. Two of the mutant groups isolated were found to accumulate 12 alpha-hydroxyandrosta-1,4-diene-3,17-dione as the major product from deoxycholic acid. Strains in one of these two groups were able to grow on steroids such as chenodeoxycholic acid, which lacks a 12 alpha-hydroxy function, whereas the one member of the second group could not. With chenodeoxycholic acid, this latter strain accumulated a yellow muconic-like derivative, tentatively identified as 3,7-dihydroxy-5,9,17-trioxo-4(5),9(10)-disecoandrosta-1(10)2 -dien-4-oic acid. Members of two further mutant groups accumulated either 12 beta-hydroxyandrosta-1,4-diene-3,17-dione or 3,12 beta-dihydroxy-9(10)-secoandrosta-1,3,5(10)-triene-9,17-dione as the major product from deoxycholic acid. The relationship between the catabolism of m- and p-cresol, 3-ethylphenol and the bile acids was also examined.
mesh_terms: D000730:Androstadienes / Q000378:metabolism; D000731:Androstanes / Q000378:metabolism*; D000733:Androstatrienes / Q000378:metabolism; D000735:Androstenedione / Q000031:analogs & derivatives / Q000378:metabolism; D001647:Bile Acids and Salts / Q000378:metabolism*; D003408:Cresols / Q000378:metabolism; D004251:DNA Transposable Elements; D009154:Mutation; D011549:Pseudomonas / Q000235:genetics / Q000378:metabolism*; D012632:Secosteroids / Q000378:metabolism*
chemical_list: D000730:Androstadienes; D000731:Androstanes; D000733:Androstatrienes; D001647:Bile Acids and Salts; D003408:Cresols; D004251:DNA Transposable Elements; D012632:Secosteroids; C053159:3,7-dihydroxy-5,9,17-trioxo-4(5),9(10)-disecoandrosta-1(10)2-dien-4-oic acid; C006573:1,4-androstadiene-3,17-dione; C046233:12-hydroxyandrosta-1,4-diene-3,17-dione; D000735:Androstenedione; C053160:3,12-dihydroxy-9(10)-secoandrosta-1,3,5(10)-triene-9,17-dione; C033936:androstane