Comparison of indirect immunofluorescence and radioimmunoassay for detecting antibodies against hepatitis A virus of the IgG and IgM classes.
Indirect Immunofluorescence test (IIF) and Radioimmunoassay (RIA) were compared for determining anti-Hepatitis A virus (HAV) IgG and IgM. 142 sera were tested for anti-HAV IgG and 16 for anti-HAV IgM, both with IIF and RIA techniques. The correlation between the results reached 98.6% for anti-HAV IgG and 93.75% for anti-HAV IgM detection, confirming the specificity and sensibility of IIF.
D000328:Adult; D005455:Fluorescent Antibody Technique; D006508:Hepatitis Antibodies / Q000032:analysis*; D006507:Hepatovirus / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis*; D007075:Immunoglobulin M / Q000032:analysis*; D011863:Radioimmunoassay
D006508:Hepatitis Antibodies; D007074:Immunoglobulin G; D007075:Immunoglobulin M