NADP+ phosphatase: a novel mitochondrial enzyme.
Mitochondria contain a NADP+ phosphatase in the matrix space. This is shown by both incubation of mitochondria and subfractions derived thereof with added NADP+ and by analysis of endogenous pyridine nucleotides after enzymatic oxidation in Ca2+-loaded mitochondria. The apparent KM for NADP+ is about 1.2 mM. NADPH is not a substrate. The enzyme may be important for modulation of posttranslational modification of macromolecules in mitochondria.
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D007700:Kinetics; D008930:Mitochondria, Liver / Q000201:enzymology*; D009708:Nucleotidases / Q000378:metabolism*; D051381:Rats
C045660:NADP phosphatase; D009708:Nucleotidases; D002118:Calcium