Age-dependent and selective binding of beta-bungarotoxin to GABAergic neurons in the rat cerebellum.
beta-Bungarotoxin (BuTx)-binding cells were immunocytochemically examined in the developing rat cerebellum. The tissue was incubated with BuTx and then immunostained with antiserum against its toxoid. On postnatal day 6, only Golgi cells were positive for immunoreaction. Immunoreactive Golgi cells were reduced in number on day 15 and disappeared on day 25. On day 15, Purkinje cells were strongly stained, while some basket and stellate cells stained weakly. On day 25 and in adult, basket and stellate cells were more immunoreactive than Purkinje cells. Thus, age-dependent and selective binding of BuTx was restricted to gamma-aminobutyric acid (GABA)ergic neurons.
D000375:Aging / Q000378:metabolism; D000818:Animals; D002038:Bungarotoxins / Q000378:metabolism*; D002525:Cerebellar Cortex / Q000378:metabolism*; D002531:Cerebellum / Q000254:growth & development; D007124:Immunoenzyme Techniques; D011689:Purkinje Cells / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D009435:Synaptic Transmission; D005680:gamma-Aminobutyric Acid / Q000502:physiology*
D002038:Bungarotoxins; D005680:gamma-Aminobutyric Acid