[Transposition of the Tn5 and Tn10 elements and duplications in Escherichia coli K12 genome].
The kinetics of accumulation of resident transposon copies in a dividing population has been defined using a special experimental system. Analysis of the kinetics made it possible to estimate the probability of transposition for Tn5 as 2.5 X 10(-4) and for Tn10 as 2.3 X 10(-6) per cell per generation. Transposition of the composite elements does not depend on RecBC or RecF pathways of recombination. The fraction of the bacterial population with tandem duplications in the proA region of the genome is permanent for Escherichia coli. It is independent of the recombination pathways (RecBC of RecF) and the integrity of DNA polymerase I.
D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D005810:Multigene Family
D004251:DNA Transposable Elements
Transpozitsiia sostavnykh élementov Tn5 i Tn10 i duplikatsiia v genome Escherichia coli K12.