title: Gene disruption indicates that the only essential function of the SKI8 chromosomal gene is to protect Saccharomyces cerevisiae from viral cytopathology.
abstract: We have cloned the SKI8 gene, one of the chromosomal genes that repress replication of M, L-A, and L-BC double-stranded RNA viruslike particles in yeast. The clone was used to map SKI8 to chromosome VII near ade5 and to construct a deletion mutant. The deletion mutant was unable to grow at 8 degrees if and only if M1 double-stranded RNA was present.
mesh_terms: D002874:Chromosome Mapping; D002875:Chromosomes / Q000502:physiology; D003001:Cloning, Molecular; D004261:DNA Replication; D004262:DNA Restriction Enzymes; D005814:Genes, Viral; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014779:Virus Replication; D014780:Viruses / Q000235:genetics*
chemical_list: D004262:DNA Restriction Enzymes