Microinjection of ras p21 induces a rapid rise in intracellular pH.
Quiescent mouse NIH 3T3 cells responded to microinjection of activated ras p21 with a rapid and sustained rise in intracellular pH (approximately 0.17 pH units). The p21-induced pH change was inhibited by amiloride treatment or growth of cells in medium low in sodium, suggesting a role for the Na+/H+ antiporter. Amiloride was found to suppress p21-induced mitosis, also.
D000584:Amiloride / Q000494:pharmacology; D000818:Animals; D002352:Carrier Proteins / Q000502:physiology; D002460:Cell Line; D004247:DNA / Q000096:biosynthesis; D004261:DNA Replication / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D051379:Mice; D008845:Microinjections; D008856:Microscopy, Fluorescence; D011518:Proto-Oncogene Proteins / Q000494:pharmacology*; D016283:Proto-Oncogene Proteins p21(ras); D051381:Rats; D017923:Sodium-Hydrogen Exchangers
D002352:Carrier Proteins; D011518:Proto-Oncogene Proteins; D017923:Sodium-Hydrogen Exchangers; D000584:Amiloride; D004247:DNA; D016283:Proto-Oncogene Proteins p21(ras)