title: Second-step reconcentration of environmental samples by ammonium sulfate flocculation of beef extract.
abstract: Some enteric viruses are sensitive to the acid environment utilized during the concentration of viruses from water. The use of a neutral flocculant, neutralized ammonium sulfate at 50% saturation, permitted the recovery of 97% of the simian rotavirus SA-11, 87% of Coxsackievirus B-4, and 88% of poliovirus type 1. This method should permit a better recovery of enteric viruses from the environment.
mesh_terms: D000645:Ammonium Sulfate; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D029822:Enterovirus B, Human / Q000302:isolation & purification*; D005427:Flocculation; D017955:Poliovirus / Q000302:isolation & purification*; D012401:Rotavirus / Q000302:isolation & purification*; D010948:Viral Plaque Assay; D014871:Water Microbiology
chemical_list: D000645:Ammonium Sulfate