SV40 small t antigen enhances the transformation activity of limiting concentrations of SV40 large T antigen.
A murine recombinant Neo(r) retrovirus encoding the SV40 small t antigen was used to infect Balb/c 3T3 CIA31 cells. From analyses of G418-resistant clones containing at least as much intact t as Cos-1 cells, we found that t, alone, had no detectable A31 transforming activity. In contrast, we noted that SV40 large T promoted A31 agar colony formation when present over a 5- to 7.5-fold concentration range. However, at the low end of the spectrum, its transforming effect was manifest inefficiently except in the presence of t. Thus a major role for t in the SV40 transforming mechanism is to enhance directly or indirectly the transforming function of T.
D000818:Animals; D000952:Antigens, Polyomavirus Transforming; D000957:Antigens, Viral, Tumor / Q000502:physiology*; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002472:Cell Transformation, Viral; D051379:Mice; D009154:Mutation; D009856:Oncogene Proteins, Viral / Q000502:physiology*; D013539:Simian virus 40 / Q000276:immunology / Q000502:physiology*
D000952:Antigens, Polyomavirus Transforming; D000957:Antigens, Viral, Tumor; D009856:Oncogene Proteins, Viral