title: High-performance liquid chromatographic separation of biological macromolecules on new silica-based ion exchangers.
abstract: The fundamental characteristics of a new series of ion-exchange columns, specifically designed for high-speed analysis of biological macromolecules, have been examined. Each of these columns is packed with a chemically modified silica gel, that has a controlled pore-size distribution and a uniform particle diameter. Surface silanol groups of these materials are chemically modified with hydrophilic polymers to produce a neutral silica surface, and ionic functional groups are chemically bonded to this neutral support, producing a stable ion exchanger. The separation of biological macromolecules on these columns has been investigated, and the selection of a mobile phase is discussed.
mesh_terms: D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D006454:Hemoglobins / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D007475:Ion Exchange Resins; D009838:Oligodeoxyribonucleotides / Q000302:isolation & purification*; D011506:Proteins / Q000302:isolation & purification*; D012822:Silicon Dioxide
chemical_list: D006454:Hemoglobins; D007202:Indicators and Reagents; D007475:Ion Exchange Resins; D009838:Oligodeoxyribonucleotides; D011506:Proteins; D012822:Silicon Dioxide