title: [Comparative study of the transformation of various thymidine kinase-deficient human and animal cell lines with the thymidine kinase gene of the Herpes simplex virus].
abstract: The comparative study of transformation of four thymidine kinase deficient cell lines (mouse mammary carcinoma cell line FS tk-; rat cell line Rat-2tk-; mouse cell line Ltk-, clone D1; human cell line 143tk-) with the thymidine kinase cloned gene of Herpes simplex virus 1 was undertaken. The differences in efficiency and optimal conditions of transformation were shown for these cell lines. The advantages and disadvantages of the cell systems examined for the use in experiments for transformation and cotransformation of cultured cells with isolated genes are discussed.
mesh_terms: D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics; D005814:Genes, Viral; D006801:Humans; D018139:Simplexvirus / Q000201:enzymology / Q000235:genetics*; D013937:Thymidine Kinase / Q000172:deficiency / Q000235:genetics*; D014162:Transfection; D014170:Transformation, Genetic
chemical_list: D004279:DNA, Viral; D013937:Thymidine Kinase
vernacular_title: Sravnitel'noe izuchenie transformatsii razlichnykh liniĭ bestimidinkinaznykh kletok cheloveka i zhivotnykh genom timidinkinazy virusa prostogo gerpesa.