5'-nucleotidase activity of murine T-cell subpopulations separated according to their Lyt2 and L3T4 phenotypes.
Murine T lymphocytes were separated by "panning" into four subpopulations, according to their Lyt2 and L3T4 phenotypes: Lyt2+L3T4+, Lyt2-L3T4-, Lyt2+L3T4-, and Lyt2-L3T4+. The activity of ecto-5'-nucleotidase in each subpopulation was measured. 5'-Nucleotidase activity was undetectable in Lyt2+L3T4+ cortical cells but was expressed in medullary Lyt2-L3T4+ and Lyt2+L3T4- T lymphocytes. The small cortical subpopulation of thymocyte precursors with the Lyt2-L3T4- phenotype expressed levels of 5'-nucleotidase comparable to the levels of medullary, mature lymphocytes. These results suggest that the use of ecto-5'-nucleotidase as a marker of the degree of T-cell maturation is questionable.
D015720:5'-Nucleotidase; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface / Q000032:analysis*; D001854:Bone Marrow Cells; D002454:Cell Differentiation; D002469:Cell Separation; D005260:Female; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009708:Nucleotidases / Q000032:analysis*; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000145:classification* / Q000201:enzymology / Q000276:immunology; D013950:Thymus Gland / Q000166:cytology
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface; D009708:Nucleotidases; D015720:5'-Nucleotidase