Regulation of the nah and sal operons of plasmid NAH7: evidence for a new function in nahR.
The catabolism of naphthalene and salicylate is specified by two operons on an 80 Kb metabolic plasmid, NAH7. These operons, nah and sal, are carried on the contiguous 30 Kb EcoRI-A, C fragments, and are under positive control of a regulator region, nahR. Five Nah Sal Tn5 insertion mutants form two complementation groups: A = nahR203, nahR204; and B = nahR201, nahR202, nahR205. The physical and genetic maps assign the nahR location to the 15.7-17.2 Kb region of the EcoRI-A fragment, with suggestion of more than one control gene.
D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant; D015247:Deoxyribonuclease HindIII; D004926:Escherichia coli / Q000235:genetics; D005809:Genes, Regulator; D009281:Naphthalenes / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D009876:Operon; D010957:Plasmids; D011549:Pseudomonas / Q000235:genetics; D012459:Salicylates / Q000378:metabolism*; D020156:Salicylic Acid
D004274:DNA, Recombinant; D009281:Naphthalenes; D012459:Salicylates; C031721:naphthalene; D004262:DNA Restriction Enzymes; D015247:Deoxyribonuclease HindIII; D020156:Salicylic Acid