[Genetic transformation of somatic cells. XI. The autonomic replication of the cloned DNA of the bovine papilloma virus in NIH/3T3 mouse fibroblasts and the changes in the growth characteristics of the transformed cells].
Mouse fibroblasts NIH 3T3 were transfected with the plasmid pBPV (142-6) containing full genome of bovine papilloma virus 1, and focuses of morphological transformation were selected 2-3 weeks later. DNA molecules, containing BPV-1 sequences, were isolated from extrachromosomal fraction of transformed clones suggesting stable autonomous replication of BPV in 3T3 NIH cells. In some rescued plasmids deletions spanning E6, 7 genes of BPV were found. It is suggested that these genes are not essential for morphological transformation and autonomous replication in 3T3 NIH cells. BPV-transformed clones are able to grow in the medium containing low concentration (0.5%) of serum.
D000818:Animals; D052688:Bovine papillomavirus 1 / Q000235:genetics*; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis; D004261:DNA Replication; D004279:DNA, Viral / Q000235:genetics*; D005347:Fibroblasts / Q000032:analysis / Q000166:cytology; D027383:Papillomaviridae / Q000235:genetics*; D010957:Plasmids
D004279:DNA, Viral; D004247:DNA
Geneticheskaia transformatsiia somaticheskikh kletok. XI. Avtonomnaia replikatsiia klonirovannoĭ DNK virusa bych'eĭ papillomy v myshinykh fibroblastakh 3T3 NIH i izmenenie rostovykh kharakteristik transformirovannykh kletok.