Detection of human T cell leukemia virus type I and human immunodeficiency virus in cultured lymphocytes of a Zairian man with AIDS.
We report the detection of human T cell leukemia virus type I (HTLV-I) and human immunodeficiency virus (HIV) in the cultured lymphocytes of a 45-year-old Zairian man with AIDS. HIV was successfully isolated and analyzed by SDS-PAGE and competition radioimmunoassay. However, by the culture techniques used, HTLV-I could not be separated from the HIV. Western blot analysis of the patient's serum showed the presence of both HTLV-I- and HIV-specific antibodies. The finding of this dual infection may explain reports that greater than or equal to 30% of patients with AIDS are positive for antibodies to HTLV-I.
D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology / Q000382:microbiology*; D000914:Antibodies, Viral / Q000032:analysis; D002460:Cell Line; D017977:Deltaretrovirus / Q000276:immunology / Q000302:isolation & purification*; D015023:Democratic Republic of the Congo; D006678:HIV / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification*; D015483:HIV Antibodies; D006801:Humans; D008297:Male; D008875:Middle Aged; D012191:Retroviridae Proteins / Q000032:analysis; D013601:T-Lymphocytes / Q000382:microbiology*; D014764:Viral Proteins / Q000032:analysis
D000914:Antibodies, Viral; D015483:HIV Antibodies; D012191:Retroviridae Proteins; D014764:Viral Proteins