title: Effects of adenosine 3',5'-cyclic monophosphate, forskolin, and cholera toxin on hormone production in human term placental cells.
abstract: Trophoblast cells from human term placenta in monolayer culture were used to investigate the role of adenosine 3',5'-cyclic monophosphate in the production of human chorionic gonadotropin and estradiol-17 beta. The intracellular concentration of adenosine 3',5'-cyclic monophosphate was elevated by (1) addition of an adenosine 3',5'-cyclic monophosphate analogue, 8-bromoadenosine 3',5'-cyclic monophosphate, (2) inhibition of the hydrolysis of Gs-GTP complex by cholera toxin, and (3) direct stimulation of adenylate cyclase with forskolin. Addition of 2 mmol/L of 8-bromoadenosine 3',5'-cyclic monophosphate markedly increased the accumulation of human chorionic gonadotropin in the culture medium on days 2, 3, and 4 of treatment. Likewise, addition of cholera toxin (0.2 microgram/ml) or forskolin (50 mumol/L) also enhanced human chorionic gonadotropin production. On the other hand, the production of estradiol-17 beta was significantly inhibited by 8-bromoadenosine 3',5'-cyclic monophosphate, at the same time that human chorionic gonadotropin production was enhanced in the same experiments. These results further support a differential role of adenosine 3',5'-cyclic monophosphate on human chorionic gonadotropin and estradiol-17 beta production in the human term placenta.
mesh_terms: D002772:Cholera Toxin / Q000494:pharmacology*; D006063:Chorionic Gonadotropin / Q000096:biosynthesis*; D005576:Colforsin / Q000494:pharmacology*; D000242:Cyclic AMP / Q000494:pharmacology*; D004958:Estradiol / Q000096:biosynthesis*; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007743:Labor, Obstetric; D010920:Placenta / Q000187:drug effects* / Q000378:metabolism; D011247:Pregnancy; D014327:Trophoblasts / Q000187:drug effects / Q000378:metabolism
chemical_list: D006063:Chorionic Gonadotropin; D005576:Colforsin; D004958:Estradiol; D002772:Cholera Toxin; D000242:Cyclic AMP