Purification of the receptor for platelet-derived growth factor from porcine uterus.
The receptor for platelet-derived growth factor has been purified to homogeneity on a large scale from porcine uterus. The purification procedure utilizes solubilization of uterus membranes by Triton X-100, followed by sequential chromatographies on wheat germ agglutinin-Sepharose, fast protein liquid chromatography Mono-Q, and anti-phosphotyrosine-Sepharose. About 160 micrograms of homogeneous and functionally active 170-kDa receptor could be purified from 5 kg of uterus tissue. The pure receptor responded to platelet-derived growth factor stimulation by autophosphorylation, indicating that the receptor has a kinase domain as an integral part of the molecule. A rabbit antiserum was produced against the pure receptor, which specifically recognizes the intact 170-kDa receptor.
D000818:Animals; D002462:Cell Membrane / Q000032:analysis; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D005260:Female; D007163:Immunosorbent Techniques; D017830:Octoxynol; D010766:Phosphorylation; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D011092:Polyethylene Glycols; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000187:drug effects / Q000302:isolation & purification* / Q000378:metabolism; D017479:Receptors, Platelet-Derived Growth Factor; D013552:Swine; D014599:Uterus / Q000032:analysis*; D014909:Wheat Germ Agglutinins
D010982:Platelet-Derived Growth Factor; D011956:Receptors, Cell Surface; D014909:Wheat Germ Agglutinins; D011092:Polyethylene Glycols; D017830:Octoxynol; D011505:Protein-Tyrosine Kinases; D017479:Receptors, Platelet-Derived Growth Factor