Induction of haem synthesis in Hep G2 human hepatoma cells by dimethyl sulphoxide. A transcriptionally activated event.
Exposure of cultured human hepatoma cells (Hep G2) to medium containing 2% (v/v) dimethyl sulphoxide resulted in an approximate doubling in the activity of delta-aminolaevulinate dehydratase, an increase in the haem content and a decreased growth rate; induced enzyme activity was decrease by 50% after treatment with alpha-amanitin. The findings are strikingly similar to those seen in murine Friend-virus-transformed erythroleukaemia cells.
D000546:Amanitins / Q000494:pharmacology; D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D002460:Cell Line; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D006418:Heme / Q000096:biosynthesis*; D006801:Humans; D008099:Liver / Q000378:metabolism*; D008113:Liver Neoplasms / Q000378:metabolism*; D000623:Porphobilinogen Synthase / Q000378:metabolism; D014158:Transcription, Genetic
D000546:Amanitins; D006418:Heme; D000623:Porphobilinogen Synthase; D004121:Dimethyl Sulfoxide