Species differences between rat and rabbit renal Na+/H+ exchangers.
Amiloride, 5-ethylisopropylamiloride (EIPA), and 5-ethylisopropyl-6-bromo-amiloride (Br-EIPA) inhibit rabbit and rat renal Na+/H+ exchangers with comparable potency. Irreversible inhibitions by Br-EIPA after irradiation of rat and rabbit renal brush-border vesicles are also similar. In contrast, irreversible inhibition by N,N'-dicyclohexylcarbodiimide (DCCD) of the rabbit renal antiporter requires higher DCCD concentrations as compared to the rat. Rabbit renal brush-border membranes show highest [14C]DCCD incorporation at MW 80,000, 51,000 and 36,000 and lack the amiloride-protectable MW 65,000 protein previously identified as Na+/H+ exchanger in rat kidney cortex. The data indicate species differences with respect to renal Na+/H+ exchangers.
D000584:Amiloride / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D004024:Dicyclohexylcarbodiimide / Q000494:pharmacology; D007668:Kidney / Q000378:metabolism*; D007700:Kinetics; D008871:Microvilli / Q000187:drug effects / Q000378:metabolism*; D011817:Rabbits; D051381:Rats; D017923:Sodium-Hydrogen Exchangers; D013045:Species Specificity
D002352:Carrier Proteins; D017923:Sodium-Hydrogen Exchangers; D004024:Dicyclohexylcarbodiimide; D000584:Amiloride