Regulation of neuroblast proliferation by hormones and growth factors in chemically defined medium.
Pure neuronal cultures prepared from 6-day-old embryonic chick brains incorporated [3H]-thymidine in serum-free medium up to the 4th day in culture. The addition of insulin any time within this culture period caused an increase in thymidine incorporation. This increase in [3H]-thymidine was correlated with an increase in cell number and percentage of labeling index. Triiodothyronine and endothelial cell growth factor were also active in stimulating [3H]-thymidine incorporation into chick neuroblasts. The effect of these trophic agents is unique since a variety of known mitogens tested were negative.
D000818:Animals; D001921:Brain / Q000166:cytology; D002455:Cell Division; D002478:Cells, Cultured; D002642:Chick Embryo; D004305:Dose-Response Relationship, Drug; D006133:Growth Substances / Q000494:pharmacology*; D007150:Immunohistochemistry; D007328:Insulin / Q000494:pharmacology; D009474:Neurons / Q000166:cytology* / Q000187:drug effects; D014284:Triiodothyronine / Q000494:pharmacology
D006133:Growth Substances; D007328:Insulin; D014284:Triiodothyronine