title: Intra-chromosomal gene conversion induced by a DNA double-strand break in Saccharomyces cerevisiae.
abstract: We have stimulated mitotic and meiotic gene conversion between non-tandem direct repeats of ADE4 by a defined double-strand break imparted in vivo to one of two copies of the gene. The experimental design permitted us to distinguish unambiguously between reciprocal intra-chromosomal crossing over and non-reciprocal break-join events that could accompany the induced conversions. We observed that (1) less than 10% of the induced conversion events are accompanied by intra-chromosomal crossing over in both mitosis and meiosis; (2) non-reciprocal break-join is not stimulated by the double-strand breaks; (3) a double-strand break in meiosis is repaired off intra-chromosomal homology (if available) with approximately sevenfold preference over repair off the homologous chromosome. Our observations, analyzed in the light of previous investigations of spontaneous inter and intra-chromosomal crossing over and gene conversion, lead to the view that chromosomal configuration constrains intra-chromosomal crossing over accompanying conversion between closely spaced repeated genes during resolution of the conversion intermediate.
mesh_terms: D002875:Chromosomes; D003434:Crossing Over, Genetic; D004271:DNA, Fungal; D005785:Gene Conversion; D008540:Meiosis; D008938:Mitosis; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics*
chemical_list: D004271:DNA, Fungal