Effects of insulin and phorbol esters on diacylglycerol generation and synthesis and hydrolysis of phosphatidylcholine in BC3H-1 myocytes.
Insulin was found to provoke simultaneous, rapid, biphasic increases in [3H]choline-labeling of phosphatidylcholine and phosphocholine in BC3H-1 myocytes. Phorbol esters increased [3H]choline-labeling of phosphocholine, but not phosphatidylcholine. Both agonists increased diacylglycerol production. These results suggest that: (a) insulin provokes coordinated increases in the synthesis and hydrolysis of PC; and, (b) insulin-induced activation of protein kinase C may activate a PC-specific phospholipase.
D000818:Animals; D002460:Cell Line; D002794:Choline / Q000378:metabolism; D004075:Diglycerides / Q000096:biosynthesis*; D005989:Glycerides / Q000096:biosynthesis*; D005990:Glycerol / Q000378:metabolism; D007328:Insulin / Q000494:pharmacology*; D007700:Kinetics; D009132:Muscles; D010713:Phosphatidylcholines / Q000378:metabolism*; D010767:Phosphorylcholine / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D004075:Diglycerides; D005989:Glycerides; D007328:Insulin; D010713:Phosphatidylcholines; D010767:Phosphorylcholine; D002794:Choline; D013755:Tetradecanoylphorbol Acetate; D005990:Glycerol