Methylation of the enhancer region of avian sarcoma virus long terminal repeat suppresses transcription.
The effect of methylation of an enhancer on transcription was studied. A 245 bp enhancer-containing a fragment of the LTR of the avian sarcoma virus was methylated in vitro and ligated back into a vector which lacked the upstream enhancer sequence. The transient expression in QT6 cells indicated that methylation of the enhancer-containing sequence severely reduced the extent of transcription.
D001358:Avian Sarcoma Viruses / Q000235:genetics*; D004279:DNA, Viral / Q000378:metabolism; D004742:Enhancer Elements, Genetic; D005809:Genes, Regulator; D008745:Methylation; D012091:Repetitive Sequences, Nucleic Acid; D014158:Transcription, Genetic
D004279:DNA, Viral