Biological activities of catfish glucagon and glucagon-like peptide.
The ability of catfish glucagon and glucagon-like peptide to bind and activate mammalian glucagon receptors was investigated. Neither catfish peptide binds to glucagon receptors of rat liver, hypothalamus or pituitary. Neither stimulates adenylate cyclase activity in liver membranes. Catfish glucagon fails to activate adenylate cyclase in hypothalamic or pituitary membranes in contrast to mammalian glucagon. However, catfish glucagon-like peptide does stimulate hypothalamic and pituitary adenylate cyclase (EC50 approximately 1 pM) possibly through mammalian glucagon-like peptide receptors.
D000262:Adenylyl Cyclases / Q000378:metabolism; D000818:Animals; D001667:Binding, Competitive; D002397:Catfishes / Q000378:metabolism*; D002462:Cell Membrane / Q000201:enzymology; D005768:Gastrointestinal Hormones / Q000378:metabolism*; D005934:Glucagon / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D004763:Glucagon-Like Peptides; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D008297:Male; D010455:Peptides / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D011964:Receptors, Gastrointestinal Hormone / Q000187:drug effects / Q000378:metabolism*; D018027:Receptors, Glucagon
D005768:Gastrointestinal Hormones; D010455:Peptides; D011964:Receptors, Gastrointestinal Hormone; D018027:Receptors, Glucagon; D004763:Glucagon-Like Peptides; C015236:glucagon-like-immunoreactivity; D005934:Glucagon; D000262:Adenylyl Cyclases