Flow cytometry of blastogenesis and the concomitant viability assay of lymphocytes stained with 4', 6 diamidino-2-phenylindole.
The blastogenic response of lymphocytes induced by concanavalin A (Con A), phytohemagglutinin (PHA), pokeweed mitogen (PWM) and by paraperiodic acid (H5IO6) was assayed by flow cytometry of 4',6 diamidino-2-phenylindole (DAPI) stained nuclei as well as by thymidine (3H-TdR) incorporation rates. The DNA content in DAPI-stained nuclei of viable and dead human, rat and mouse lymphocytes, was readily distinguishable from each other by flow cytometric methods (FCM).
D000818:Animals; D002469:Cell Separation; D002470:Cell Survival; D003208:Concanavalin A / Q000494:pharmacology; D004247:DNA / Q000096:biosynthesis; D005260:Female; D005434:Flow Cytometry; D005456:Fluorescent Dyes; D006801:Humans; D007211:Indoles; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000166:cytology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010504:Periodic Acid / Q000494:pharmacology; D010835:Phytohemagglutinins / Q000494:pharmacology; D011043:Pokeweed Mitogens / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains
D005456:Fluorescent Dyes; D007211:Indoles; D010835:Phytohemagglutinins; D011043:Pokeweed Mitogens; D010504:Periodic Acid; D003208:Concanavalin A; C007293:DAPI; D004247:DNA