Suppression of in vitro neutrophil function by feline leukaemia virus (FeLV) and purified FeLV-p15E.
Feline neutrophils (PMN) were isolated and exposed to ultraviolet light-inactivated feline leukaemia virus (UV-FeLV) and purified envelope component p15E (FeLV-p15E). Functional capacity of exposed PMN was measured in vitro utilizing the chemiluminescence (CL) response. PMN exposed to UV-FeLV demonstrated depressed CL responses to Ca2+-ionophore A23187 and latex particles. However, FeLV-p15E produced significant suppression in the CL response to A23187 but failed to produce significant alterations in response to latex particles. The data indicate that FeLV-p15E may, in part, be responsible for increased morbidity and mortality among FeLV-infected cats through suppression of the PMN population.
D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000502:physiology; D002415:Cats; D007108:Immune Tolerance; D007111:Immunity, Cellular; D007840:Latex; D005256:Leukemia Virus, Feline / Q000276:immunology / Q000502:physiology* / Q000528:radiation effects; D008163:Luminescent Measurements; D008863:Microspheres; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology*; D012191:Retroviridae Proteins / Q000502:physiology*; D015682:Retroviridae Proteins, Oncogenic; D014466:Ultraviolet Rays
D007840:Latex; D012191:Retroviridae Proteins; D015682:Retroviridae Proteins, Oncogenic; C051525:feline leukemia virus protein p15E; D000001:Calcimycin; D002118:Calcium