An enzyme catalyzing the liberation of N-acetylglucosamine from N-acetylglucosaminyl pyrophosphorylpolyprenol in Bacillus polymyxa membranes.
A novel enzyme which specifically hydrolyzes N-acetylglucosaminyl pyrophosphorylpolyprenol to liberate N-acetylglucosamine was found in membranes of Bacillus polymyxa AHU 1385. The enzyme seems to be inactive toward alpha-N-acetylglucosaminyl phosphorylundecaprenol, beta-N-acetylglucosaminyl phosphorylundecaprenol, N-acetylglucosamine 1-phosphate, N-acetylglucosamine 1-pyrophosphate, or UDP-N-acetylglucosamine. Much lower activities of the same enzyme were also found in membranes of several other strains of Bacilli.
D000117:Acetylglucosamine / Q000378:metabolism*; D001407:Bacillus / Q000201:enzymology*; D001426:Bacterial Proteins / Q000302:isolation & purification* / Q000378:metabolism; D005944:Glucosamine / Q000031:analogs & derivatives*; D006868:Hydrolysis; D008565:Membrane Proteins / Q000302:isolation & purification* / Q000378:metabolism; D010727:Phosphoric Diester Hydrolases / Q000302:isolation & purification* / Q000378:metabolism; D011103:Polyisoprenyl Phosphate Monosaccharides / Q000378:metabolism*; D011105:Polyisoprenyl Phosphate Sugars / Q000378:metabolism*; D013045:Species Specificity; D013379:Substrate Specificity
D001426:Bacterial Proteins; D008565:Membrane Proteins; D011103:Polyisoprenyl Phosphate Monosaccharides; D011105:Polyisoprenyl Phosphate Sugars; C018439:N-acetylglucosaminylpyrophosphoryldolichol; C053109:N-acetylglucosaminyl pyrophosphorylpolyprenol hydrolase; D010727:Phosphoric Diester Hydrolases; D005944:Glucosamine; D000117:Acetylglucosamine