Cloning a cDNA for Drosophila melanogaster urate oxidase.
A cDNA library from third-instar larval Malpighian tubules of Drosophila melanogaster was constructed and screened for urate oxidase (UO) clones by hybridization selection. The coding sequence for UO was mapped by in situ hybridization to position 28C on the left arm of chromosome 2. The UO activity in Drosophila shows a complex developmental profile. A UO cDNA was used as a probe of Northern blots of poly(A) + RNA from various stages of development. The data show that there is a direct correlation between the transcriptional activity of the UO locus as evidenced by the quantitative changes of UO mRNA and the levels of UO activity and protein during development.
D000818:Animals; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism* / Q000648:ultrastructure; D004262:DNA Restriction Enzymes; D004331:Drosophila melanogaster / Q000201:enzymology / Q000235:genetics*; D005796:Genes; D008317:Malpighian Tubules / Q000201:enzymology; D009693:Nucleic Acid Hybridization; D014503:Urate Oxidase / Q000235:genetics*
D004247:DNA; D014503:Urate Oxidase; D004262:DNA Restriction Enzymes