Episomal maintenance of a bovine papilloma virus vector in transgenic mice.
We have used a bovine papillomavirus-based vector to generate transgenic mice. Transgenic mice result from either pronuclear or cytoplasmic injections of the vector into fertilized eggs. Of 30 mice generated by microinjection, 27 (90%) contained the vector in its episomal form, at less than one copy per cell. This represents a highly efficient means of gene transfer in which the transgene is in a controlled genetic environment.
D000818:Animals; D052688:Bovine papillomavirus 1 / Q000235:genetics; D004624:Embryo Transfer; D005260:Female; D005786:Gene Expression Regulation; D005814:Genes, Viral; D005822:Genetic Vectors; D008297:Male; D051379:Mice; D008845:Microinjections; D010957:Plasmids; D011247:Pregnancy; D014170:Transformation, Genetic