Genotypic analysis of large cell lymphomas which express the Ki-1 antigen.
The monoclonal antibody Ki-1 reacts with Reed-Sternberg cells in Hodgkin's disease and with the tumour cells in a minority of large cell non-Hodgkin's lymphomas. This study describes the results of immunophenotypic and DNA analysis in 30 cases of non-Hodgkin's lymphoma, all of which expressed the Ki-1 antigen. The genotypic analysis has been undertaken using both immunoglobulin and T-cell receptor gene probes. Sixteen cases were shown by this method to be of monoclonal T-cell origin, six of B-cell origin, while in eight cases there was no evidence of either T- or B-cell lineage. This confirms previous immunohistological data indicating that non-Hodgkin's lymphomas which express the Ki-1 antigen may be of either T-cell or B-cell origin.
D000293:Adolescent; D000328:Adult; D000368:Aged; D000951:Antigens, Neoplasm / Q000032:analysis*; D001345:Autoradiography; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D005802:Genes, MHC Class II; D005838:Genotype; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D017730:Ki-1 Antigen; D008228:Lymphoma, Non-Hodgkin / Q000235:genetics / Q000276:immunology / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D011948:Receptors, Antigen, T-Cell / Q000276:immunology
D000951:Antigens, Neoplasm; D004273:DNA, Neoplasm; D007074:Immunoglobulin G; D017730:Ki-1 Antigen; D011948:Receptors, Antigen, T-Cell