Studies of the MHC class I molecules of veto-active and veto-inactive lectin-stimulated spleen cell populations.
Mouse spleen cells activated for three days with ConA, but not with PHA and LPS, were found to possess strong veto activity. Thus, ConA-activated spleen cells were capable of preventing a cytotoxic T cell response generated in MLC against the H-2 haplotype of the ConA-activated cells. Radiolabelling and chemical cross-linking of veto-active and veto-inactive cells, followed by immunoprecipitation of H-2, class I antigens, and analyses of the precipitates by one and two dimensional SDS PAGE were performed. By these procedures we were unable to demonstrate qualitative and quantitative differences between precipitates of veto-active and veto-inactive cell populations.
D000818:Animals; D000954:Antigens, Surface / Q000502:physiology*; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D003432:Cross-Linking Reagents; D004591:Electrophoresis, Polyacrylamide Gel; D006680:HLA Antigens / Q000502:physiology*; D007158:Immunologic Techniques; D037102:Lectins / Q000494:pharmacology; D008715:Methionine; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000166:cytology* / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D000954:Antigens, Surface; D003432:Cross-Linking Reagents; D006680:HLA Antigens; D037102:Lectins; D003208:Concanavalin A; D008715:Methionine