Regulation of cytoplasmic poly(A) polymerase activity by dihydrotestosterone in the kidney of rat.
Polyadenylation of mRNA, a post-transcriptional phenomenon, is catalysed by cytoplasmic and nuclear poly(A) polymerases. During normal growth and development cytoplasmic poly(A) polymerase activity in the kidney of rat increased from day 20 reaching to a peak at 35 days. Dihydrotestosterone at a dose of 500 micrograms and above significantly stimulated the enzyme activity in the cortex of kidney in the castrated animals. A single dose of dihydrotestosterone caused significant increase in the enzyme activity at 12 h after treatment and was maintained till 24 h. Antiandrogen cyproterone acetate inhibited dihydrotestosterone-induced enzyme activity in kidney cortex. These results show that cytoplasmic poly(A) polymerase of rat kidney is regulated by dihydrotestosterone.
D000818:Animals; D003534:Cyproterone / Q000031:analogs & derivatives / Q000494:pharmacology; D017373:Cyproterone Acetate; D003593:Cytoplasm / Q000201:enzymology*; D013196:Dihydrotestosterone / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D006128:Growth; D007668:Kidney / Q000201:enzymology*; D008297:Male; D009713:Nucleotidyltransferases / Q000378:metabolism*; D011062:Polynucleotide Adenylyltransferase / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D013196:Dihydrotestosterone; D017373:Cyproterone Acetate; D003534:Cyproterone; D009713:Nucleotidyltransferases; D011062:Polynucleotide Adenylyltransferase