Artifactual phosphate binding due to impurities in [32P]orthophosphate.
Many commercial preparations of [32P]orthophosphate contain radioactive impurities that interfere with binding and transport studies in biological systems. One type of impurity is micro-particulate whereas another may be pyrophosphate. Methods of removing these impurities from radiolabeled orthophosphate solutions are described.
D011756:Diphosphates / Q000302:isolation & purification; D010710:Phosphates / Q000302:isolation & purification* / Q000378:metabolism*; D010761:Phosphorus Radioisotopes
D011756:Diphosphates; D010710:Phosphates; D010761:Phosphorus Radioisotopes