Direct analytical and preparative resolution of enantiomers using albumin adsorbed to silica as a stationary phase.
A rapid and simple method for the preparation of high-performance liquid chromatography columns for chiral separations is described. The stationary phase is prepared by adsorbing bovine serum albumin on silica. Both analytical and preparative applications are described. A polarimeter was used as a detector to determine the enantiomer elution orders.
D000327:Adsorption; D000818:Animals; D002417:Cattle; D006863:Hydrogen-Ion Concentration; D004364:Pharmaceutical Preparations / Q000302:isolation & purification*; D012710:Serum Albumin, Bovine; D012822:Silicon Dioxide; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism
D004364:Pharmaceutical Preparations; D012710:Serum Albumin, Bovine; D012822:Silicon Dioxide