title: Establishment of monoclonal antibodies reactive with epithelial and myoepithelial cells in the breast.
abstract: Monoclonal antibodies which are considered to be able to differentiate epithelial and myoepithelial cells in the breast have been developed. Human mammary carcinoma cell line (HBC-4W) was used for immunization. Monoclonal antibodies-B4B2F10 (epi-1), E9E8B7 (myo-1)-with IgM was examined using tissues from diseased breast by avidin-biotin-peroxidase assay. Epi-1 antibody reacted with epithelial cells while myo-1 antibody reacted with myoepithelial cells in the mammary glands, respectively. The reaction was markedly visible, in particular, in fibroadenoma, mastopathy, and papilloma, which showed clear two-cell-type structures. In the infiltrating ductal carcinoma, epi-1 antibody reacted with carcinoma cells, while myo-1 antibody reacted with stromal cells rather than carcinoma cells suggesting that infiltrating ductal carcinoma was mainly of epithelial origin. In the infiltrating lobular carcinoma, however, myo-1 as well as epi-1 antibodies reacted with carcinoma cells. It is suggested that infiltrating lobular carcinoma was of a mixture of epithelial and myoepithelial cell origin.
mesh_terms: D000232:Adenofibroma / Q000032:analysis / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal; D001941:Breast Diseases / Q000276:immunology; D001943:Breast Neoplasms / Q000032:analysis / Q000276:immunology*; D002285:Carcinoma, Intraductal, Noninfiltrating / Q000032:analysis / Q000276:immunology*; D002460:Cell Line; D005260:Female; D006801:Humans; D006825:Hybridomas; D007124:Immunoenzyme Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D010212:Papilloma / Q000032:analysis / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal