The ornithine requirement of urea synthesis. Formation of ornithine from glutamine in hepatocytes.
In hepatocytes, urea synthesis from glutamine is independent of added ornithine, even when rates are high after stimulation of glutamine metabolism by dibutyryl cyclic AMP, phenylephrine or vasopressin. Incubation with glutamine increases tissue [ornithine]. The increases parallel those of [N-acetylglutamate] under different conditions. The ornithine requirement of urea synthesis increases with increasing supply of ammonia. A function of the unique, highly regulated, glutaminase of liver may be to regulate ornithine synthesis.
D000643:Ammonium Chloride / Q000378:metabolism; D000818:Animals; D003994:Bucladesine / Q000378:metabolism; D005260:Female; D005971:Glutamates / Q000378:metabolism; D005973:Glutamine / Q000378:metabolism*; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism*; D009952:Ornithine / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D014508:Urea / Q000096:biosynthesis*
D005971:Glutamates; D000643:Ammonium Chloride; D005973:Glutamine; D003994:Bucladesine; D014508:Urea; D009952:Ornithine; C016195:N-acetylglutamic acid