Polymyxin B binding sites in Escherichia coli as revealed by polymyxin B-gold labeling.
A complex of polymyxin B, bovine serum albumin, and colloidal gold was prepared and used for the ultrastructural localization of polymyxin B binding sites on thin sections of Epon-embedded Escherichia coli cells. Gold particles were found on the outer membrane of E. coli, which is consistent with reported biochemical findings. We concluded that gold labeling with polymyxin B is useful in localizing the binding sites of polymyxin.
D001665:Binding Sites; D003102:Colloids; D004926:Escherichia coli / Q000378:metabolism*; D006046:Gold; D006651:Histocytochemistry / Q000379:methods*; D008854:Microscopy, Electron; D011112:Polymyxin B / Q000378:metabolism*; D011113:Polymyxins / Q000378:metabolism*
D003102:Colloids; D011113:Polymyxins; D006046:Gold; D011112:Polymyxin B