title: The kidney in potassium depletion. II. K+ handling by the isolated perfused rat kidney.
abstract: In a companion paper we reported a large increment in Na+-K+-ATPase activity and [3H]ouabain binding in the inner stripe of outer medullary collecting tubules from K-depleted rats. To test the hypothesis that the increased number of Na+-K+ pumps in these animals may be involved in potassium reabsorption we examined the effect of ouabain on K excretion by isolated, perfused kidneys from rats fed a K-free diet for 3 wk. Kidneys from K-depleted rats retain potassium avidly, both the fractional (FEK) and absolute K excretion being approximately fivefold lower than in control kidneys. Ouabain (5 mM) increased FEK in kidneys from each K-depleted rat [mean from 8.5 to 34.4%, P less than 0.001, at average (4.5 mM) perfusate K]; similar results were obtained when kidneys were perfused with low (approximately 2 mM) and high (greater than or equal to 8 mM) potassium concentrations. In contrast, ouabain produced a variable effect in control kidneys, that depended on the perfusate potassium concentration. In K-depleted rats amiloride (10(-4) M) did not significantly alter K excretion and did not block the ouabain-induced kaliuresis, suggesting that the latter is not due to enhanced secretion secondary to increased distal fluid delivery. These results provide evidence for ouabain-sensitive potassium reabsorption in kidneys of chronically K-depleted rats, and suggest an explanation for the increased Na+-K+-ATPase observed in such animals.
mesh_terms: D000584:Amiloride / Q000494:pharmacology; D000818:Animals; D007684:Kidney Tubules / Q000378:metabolism*; D007685:Kidney Tubules, Collecting / Q000378:metabolism*; D008297:Male; D008954:Models, Biological; D010042:Ouabain / Q000378:metabolism; D010477:Perfusion; D011188:Potassium / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism
chemical_list: D010042:Ouabain; D000584:Amiloride; D000254:Sodium-Potassium-Exchanging ATPase; D011188:Potassium