[An immunoenzyme test used for the detection of antibodies to rinderpest: the advantage of using a purified virus cultivated in a cell line].
Two types of in vitro assays (enzyme immunoassay and sero-neutralization test) were compared for their ability to detect antibodies to rinderpest virus in field sera from West African bovines. Purified virus grown on Vero cells (Ag/Vero) or bovine kidney cells (Ag/BK), were tested as antigen in enzyme-linked immunoassays (EIA). The results of the comparative evaluation of the two antigens by EIA, prove that Ag/Vero did enhance the sensitivity and the specificity of the test in comparison to Ag/BK and offers a 94% correspondence with seroneutralization.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D002417:Cattle; D002460:Cell Line; D007124:Immunoenzyme Techniques; D009500:Neutralization Tests; D011237:Predictive Value of Tests; D012301:Rinderpest / Q000276:immunology*; D012302:Rinderpest virus / Q000276:immunology*; D014709:Vero Cells
D000914:Antibodies, Viral
Un test immunoenzymatique appliqué à la détection des anticorps dirigés contre la peste bovine: l'interêt d'utiliser un virus purifié cultivé sur cellules de lignée.