Expression of a provirus of human T cell leukaemia virus type I by DNA transfection.
We isolated the full length provirus of human T cell leukaemia virus type I (HTLV-I) from MT-2, a lymphoid cell line producing HTLV-I. In three non-lymphoid cell lines (COS7, human osteosarcoma HOS cells, and HeLa) this provirus expressed a trans-acting activity after co-transfection with a recombinant plasmid carrying a bacterial chloramphenicol acetyltransferase gene under the control of a long terminal repeat of HTLV-I provirus. The trans-acting protein p40 was detected by immunoprecipitation in transfected HOS cells. Structural proteins of HTLV-I, the gag and env products, were also formed and processed in the same manner as observed in MT-2 cells. In transfected HeLa cells, the p40 protein was mainly localized in the nucleus, while other structural proteins were detected in the cytoplasm and/or the membrane by indirect immunofluorescence. Syncytium formation was observed in HeLa cells after transfection. These results demonstrated that non-lymphoid cells could produce the major proteins of HTLV-I after DNA transfection of the cloned provirus.
D000956:Antigens, Viral / Q000032:analysis; D002459:Cell Fusion; D002460:Cell Line; D004274:DNA, Recombinant; D004279:DNA, Viral / Q000235:genetics*; D017977:Deltaretrovirus / Q000235:genetics* / Q000276:immunology / Q000502:physiology; D006367:HeLa Cells; D006801:Humans; D008214:Lymphocytes / Q000382:microbiology; D012516:Osteosarcoma; D014162:Transfection; D014764:Viral Proteins / Q000096:biosynthesis; D014779:Virus Replication
D000956:Antigens, Viral; D004274:DNA, Recombinant; D004279:DNA, Viral; D014764:Viral Proteins