[Expressing plasmid vectors on the basis of Escherichia coli beta-galactosidase gene fragments].
With the use of synthetic DNA fragments, a set of new plasmid vectors has been obtained. The vectors provided high level expression of peptides and small proteins in E. coli as fusions with fragments of beta-galactosidase of various length. These vectors were used to achieve expression of a synthetic gene for a functionally active fragment of bacteriorhodopsin. The yields of hybrid proteins consisting of beta-galactosidase and bacteriorhodopsin fragments were in the range of 5-30% from the total amount of cellular protein.
D001436:Bacteriorhodopsins / Q000096:biosynthesis / Q000235:genetics; D001483:Base Sequence; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D005696:Galactosidases / Q000235:genetics*; D005786:Gene Expression Regulation; D005798:Genes, Bacterial; D005813:Genes, Synthetic; D005822:Genetic Vectors; D010957:Plasmids; D001616:beta-Galactosidase / Q000235:genetics*
D004269:DNA, Bacterial; D001436:Bacteriorhodopsins; D004262:DNA Restriction Enzymes; D005696:Galactosidases; D001616:beta-Galactosidase
Ekspressiruiushchie plazmidnye vektory na osnove fragmentov gena beta-galaktozidazy Escherichia coli.