A simple method for extraction of DNA from fungi and yeasts with anhydrous hydrogen fluoride.
A novel method is described for the extraction of DNAs from fungi and yeasts. Anhydrous hydrogen fluoride (HF) selectively cleaves their cell walls under mild conditions (for 5 min at 0 degrees C), enabling the effective extraction of DNAs from organisms with a cell wall. A possible mechanism for this method concerning the selective cleavage of O-glycosidic linkages in cell walls has been described previously [(1977) Anal. Biochem. 82,289-309]. The extracted DNA is intact: in fact, the yeast DNA is directly applicable for restriction analysis and transformation of Escherichia coli.
D004262:DNA Restriction Enzymes; D004271:DNA, Fungal / Q000235:genetics / Q000302:isolation & purification*; D004926:Escherichia coli / Q000235:genetics; D006858:Hydrofluoric Acid; D008970:Molecular Weight; D010957:Plasmids; D014170:Transformation, Genetic; D015003:Yeasts / Q000032:analysis / Q000235:genetics
D004271:DNA, Fungal; D004262:DNA Restriction Enzymes; D006858:Hydrofluoric Acid