Synthesis in yeast of hepatitis B virus surface antigen modified P31 particles by gene modification.
The pre-S2 portion of hepatitis B virus surface antigen P31 gene was modified to make gene products resistant to trypsin-like proteases in Saccharomyces cerevisiae. The coding sequence for 6 amino acids (Ser44 - Thr49) including Arg48 was removed, and the altered gene was inserted into an expression vector. The modified HBsAg P31 (M-P31c) gene products, consisting of GP37 and GP34, formed particles having both HBsAg antigenicity and polymerized-albumin receptor activity. Since the M-P31c particles can elicite two kinds of protective antibodies against hepatitis B virus, anti-S and anti-pre-S2 antibodies, the M-P31c particles are expected to be potentially effective to S-nonresponders.
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D006514:Hepatitis B Surface Antigens / Q000235:genetics* / Q000276:immunology; D006801:Humans; D051379:Mice; D009154:Mutation; D010447:Peptide Hydrolases / Q000494:pharmacology; D010957:Plasmids; D017457:Receptors, Albumin; D011956:Receptors, Cell Surface / Q000276:immunology; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D013045:Species Specificity; D014612:Vaccines / Q000276:immunology
D006514:Hepatitis B Surface Antigens; D017457:Receptors, Albumin; D011956:Receptors, Cell Surface; D014612:Vaccines; D010447:Peptide Hydrolases