title: Products of cells cultured from gliomas. V. Cytology and morphometry of two cell types cultured from glioma.
abstract: Explants of cells of a human glioma were evaluated with the nuclear fluorochrome 4',6-diamidino-2-phenylindole, by phase-contrast illumination, and by Giemsa staining correlated with double immunofluorescence for glial fibrillary acidic protein (GFAP) and fibronectin (FN). FN-positive (FN+) cells lacked GFAP detectable by immunofluorescence. Their mean nuclear-to-cytoplasmic ratio was large (0.192). Actual mean areas of nuclei (1,252 microns2) and cytoplasm (8,376 microns2) of FN+ cells compared with mean areas of fibroblasts suggested that the high nuclear-to-cytoplasmic ratio of FN+ cells was due to their microscopically evident reduced cytoplasmic spreading rather than to larger nuclei. Some FN+ cells showed marked variation in nuclear and nucleolar size and shape. Others had abnormal mitoses or hyperchromatic nuclei. GFAP-positive (GFAP+) cells lacked FN detectable by immunofluorescence. GFAP+ cells were smaller and less round than FN+ cells. Their usual location was growing on a layer of FN+ cells. The mean nuclear-to-cytoplasmic ratio (0.245) of GFAP+ cells was the highest in the study, surpassing the ratio of the continuous glioma line LM (0.176). Mean areas of nuclei (289 microns2) and of cytoplasm (1,350 microns2) of GFAP+ cells suggested that their high nuclear-to-cytoplasmic ratio was due to their microscopically evident reduced cytoplasmic spreading. Reduced spreading was associated with extension of long, thin cytoplasmic processes. The majority of GFAP+ cells showed marked cytoplasmic basophilia, nuclear hyperchromasia, and clumped chromatin. Features observed in both FN+ and GFAP+ cells from this high-grade astrocytoma are features associated with malignant transformation in more thoroughly studied tumor systems.
mesh_terms: D000951:Antigens, Neoplasm / Q000032:analysis; D001932:Brain Neoplasms / Q000032:analysis / Q000276:immunology / Q000473:pathology*; D002467:Cell Nucleus / Q000648:ultrastructure; D002478:Cells, Cultured; D003593:Cytoplasm / Q000648:ultrastructure; D005353:Fibronectins / Q000032:analysis; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D005909:Glioblastoma / Q000032:analysis / Q000276:immunology / Q000473:pathology*; D006801:Humans; D009363:Neoplasm Proteins / Q000032:analysis; D012334:RNA, Neoplasm / Q000032:analysis
chemical_list: D000951:Antigens, Neoplasm; D005353:Fibronectins; D005904:Glial Fibrillary Acidic Protein; D009363:Neoplasm Proteins; D012334:RNA, Neoplasm