Collagen biosynthesis and degradation during deposit and resorptive phases of carrageenin granuloma.
Collagen metabolism was studied in both, the developing and resorptive phases of carrageenin granuloma. The rate of collagen biosynthesis relative to the rate of total protein synthesis is almost twice as high during the deposit phase compared with the resorptive phase of the same process. Total collagenolytic activity appears to be approximately the same in both, deposit and resorption stages of the granuloma. Notwithstanding, the enzyme is fully active during resorption; whereas half of this activity is in a latent form during deposit. Collagenolytic activity increased remarkably when assayed at 30 mM CaCl2 as compared to 10 or 50 mM.
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002351:Carrageenan / Q000008:administration & dosage / Q000009:adverse effects*; D003094:Collagen / Q000096:biosynthesis* / Q000378:metabolism; D006099:Granuloma / Q000139:chemically induced / Q000201:enzymology / Q000378:metabolism*; D006168:Guinea Pigs; D007279:Injections, Subcutaneous; D008297:Male; D003012:Microbial Collagenase / Q000378:metabolism; D013997:Time Factors
D002351:Carrageenan; D003094:Collagen; D003012:Microbial Collagenase; D002118:Calcium