Isolation of intermediates in the binding of the FLP recombinase of the yeast plasmid 2-micron circle to its target sequence.
We describe a method for isolating and characterizing intermediates in the binding of the FLP recombinase, encoded by the yeast plasmid 2-micron circle to its target sequence. On a wild-type substrate, three specific complexes are formed. Footprinting analysis of the gel-purified complexes shows that each complex is the result of a unique FLP-DNA association. On the basis of the behavior of various FLP target sequences in the gel-binding assay, we propose a model describing the steps that lead to the formation of a stable FLP-DNA complex.
D001483:Base Sequence; D004247:DNA / Q000378:metabolism*; D004254:DNA Nucleotidyltransferases / Q000378:metabolism*; D004251:DNA Transposable Elements; D008957:Models, Genetic; D009154:Mutation; D010957:Plasmids; D013997:Time Factors; D015003:Yeasts / Q000235:genetics*
D004251:DNA Transposable Elements; D004247:DNA; D004254:DNA Nucleotidyltransferases; C045119:FLP recombinase