Herpes simplex virus type 2 glycoprotein biogenesis: effect of monensin on glycoprotein maturation, intracellular transport and virus infectivity.
The ionophore monensin inhibited the formation of herpes simplex virus type 2 (HSV-2) particles by about 30% but the yields of infectious particles were reduced to 5% and 1% for cell-associated and extracellular virus, respectively. The presence of monensin did not affect the processing of the two viral glycoproteins gB-2 and gG-2. However, two other glycoproteins, gC-2 and gD-2, were not processed to their fully mature forms in the monensin-treated cells and only the faster moving pgC-2 and pgD-2 were detected. The cell-associated virus particles contained the glycoproteins gB-2, gC-2, gD-2 and gG-2, whereas the extracellular virus particles contained only gG-2 glycoprotein. These results suggest that HSV-2 particles containing all the viral glycoproteins are transported via the Golgi apparatus to the cell surface but that virus particles containing only gG-2 may follow a different pathway for transport and release.
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D006224:Cricetinae; D005347:Fibroblasts / Q000378:metabolism; D006023:Glycoproteins / Q000096:biosynthesis* / Q000502:physiology; D006056:Golgi Apparatus / Q000502:physiology; D007668:Kidney; D008647:Mesocricetus; D008985:Monensin / Q000494:pharmacology*; D009844:Oligosaccharides / Q000378:metabolism; D011499:Protein Processing, Post-Translational / Q000187:drug effects*; D018139:Simplexvirus / Q000378:metabolism* / Q000472:pathogenicity; D014709:Vero Cells; D014759:Viral Envelope Proteins / Q000096:biosynthesis* / Q000502:physiology; D014771:Virion / Q000032:analysis; D014779:Virus Replication / Q000187:drug effects
D006023:Glycoproteins; D009844:Oligosaccharides; D014759:Viral Envelope Proteins; D008985:Monensin