A strong homology exists between the active T-cell binding gp120 octapeptide of human immunodeficiency virus and the subtilisin cleavage peptide of bovine ribonuclease A.
A homology has been found between an octapeptide involved in attachment of the human immunodeficiency virus to helper/inducer T cells and an octapeptide segment of bovine pancreatic ribonuclease A. This segment (residues 19-26) contains the sites for subtilisin cleavage of this enzyme into the S-peptide and S-protein. From the X-ray crystal structure of ribonuclease, this sequence is known to be exposed to solvent and interacts little with the rest of the protein. A structure for the human immunodeficiency virus attachment peptide can be deduced from this homology, as a well-defined structure has been determined for this sequence in ribonuclease. This can be readily accomplished using previously developed computer methods based upon conformational energy calculations. The calculated structure for human immunodeficiency virus peptide is identical to the ribonuclease segment (19-26) in backbone conformation. It is stabilized by internal interactions of nonpolar residues, and by exposure of polar hydroxyl groups. The results suggest that the T-cell human immunodeficiency virus receptor may be hydrophilic in nature and that conservation of the sequence in two presumably functionally unrelated proteins is related to the need for conservation of exposed structure.
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D006678:HIV / Q000276:immunology*; D015699:HIV Envelope Protein gp120; D006801:Humans; D008958:Models, Molecular; D010446:Peptide Fragments / Q000235:genetics*; D011487:Protein Conformation; D015728:Receptors, HIV; D011991:Receptors, Virus / Q000276:immunology*; D012191:Retroviridae Proteins / Q000235:genetics*; D012259:Ribonuclease, Pancreatic / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D013601:T-Lymphocytes / Q000276:immunology*
D015699:HIV Envelope Protein gp120; D010446:Peptide Fragments; D015728:Receptors, HIV; D011991:Receptors, Virus; D012191:Retroviridae Proteins; C051851:ribonuclease A (19-26); D012259:Ribonuclease, Pancreatic