Reactivation of herpes simplex virus is associated with production of a low molecular weight factor that inhibits lymphokine activity in vitro.
Peripheral blood mononuclear cells obtained during recrudescent Herpes simplex virus (HSV) infection and stimulated with UV-inactivated viral antigen (UV-HSV) for 24 hr produced a low molecular weight (dialyzable) factor that inhibited lymphokine activity. This factor prevented the expression of leukocyte inhibitory factor (LIF) activity, but not its production. It was not made in UV-HSV-stimulated cultures grown in presence of 2 X 10(-6) M indomethacin nor in cultures of peripheral blood mononuclear cells obtained during convalescence or quiescence (greater than 4 days from onset of clinical symptoms) or from seropositive controls without a history of recurrent HSV disease. Dialyzable inhibitory factor production required OKM1+, OKT8+, and OKIa+ cells as determined by complement-mediated lysis with monoclonal antibody. Dialyzable inhibitory factor activity was associated with a trypsin-sensitive 8.2 K fraction as determined by Sephadex chromatography followed by sodium dodecyl sulfate-acrylamide gel electrophoresis.
D000956:Antigens, Viral / Q000276:immunology / Q000528:radiation effects; D002478:Cells, Cultured; D003289:Convalescence; D006561:Herpes Simplex / Q000097:blood / Q000276:immunology*; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology; D008222:Lymphokines / Q000037:antagonists & inhibitors*; D008970:Molecular Weight; D009000:Monocytes / Q000145:classification / Q000276:immunology; D012008:Recurrence; D018139:Simplexvirus / Q000254:growth & development* / Q000276:immunology; D014466:Ultraviolet Rays; D014775:Virus Activation
D000956:Antigens, Viral; D008222:Lymphokines; C010598:leukocyte inhibitory factor; D007213:Indomethacin