A multidot immunobinding assay for the serodiagnosis of tuberculosis. Comparison with an enzyme-linked immunosorbent assay.
A simple dot immunobinding (dot blot) assay procedure has been developed for the detection of antibodies directed against a soluble mycobacterial antigen preparation. This technique was compared with the widely used ELISA, in a study of samples from tuberculous patients. Dot blots were read on a densitometer. The correlation between both assays was excellent (r = 0.91; P less than 0.001); 90% of sera from tuberculous patients were detected using both techniques and a serial two-fold dilution method. Assessments of the end-points of titration curves by reflectometry and simple visual interpretation gave similar results. The dot blot assay is easier to perform and appears to be a practical alternative to ELISA for the detection of anti-mycobacterial antibodies in tuberculous patients.
D000328:Adult; D000907:Antibodies, Bacterial / Q000032:analysis; D003720:Densitometry; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D015151:Immunoblotting / Q000379:methods; D009163:Mycobacterium bovis / Q000276:immunology; D012698:Serologic Tests / Q000379:methods; D012882:Skin Tests; D014376:Tuberculosis / Q000175:diagnosis* / Q000276:immunology
D000907:Antibodies, Bacterial