Role of the agnoprotein in regulation of simian virus 40 replication and maturation pathways.
Analysis of two agnogene mutants, dl2304 deleted over the entire agnogene and in2379 carrying a 2-base insert, indicated that the mutant phenotype of small plaque formation must be the result of a defect late in the maturation pathway. Both mutants were removed from the pool of molecules available for replication with wild-type kinetics. Whereas dl2304 was somewhat reduced in its rate of progression from chromatin to previrions-virions, in2379, which produced even smaller plaques than dl2304 did, progressed with wild-type kinetics. Therefore, the agnoprotein was not required for progression from chromatin to previrions.
D002843:Chromatin / Q000378:metabolism; D004279:DNA, Viral / Q000502:physiology; D004268:DNA-Binding Proteins / Q000502:physiology*; D005786:Gene Expression Regulation; D009024:Morphogenesis; D013539:Simian virus 40 / Q000502:physiology*; D014764:Viral Proteins / Q000502:physiology*; D054334:Viral Regulatory and Accessory Proteins; D014779:Virus Replication; D000086082:Viroporin Proteins
D002843:Chromatin; D004279:DNA, Viral; D004268:DNA-Binding Proteins; D014764:Viral Proteins; D054334:Viral Regulatory and Accessory Proteins; C042559:agnoprotein, polyomavirus; D000086082:Viroporin Proteins