Colchicine acts as a progression factor to initiate DNA synthesis in quiescent Balb/c 3T3 cells.
In quiescent Balb/c 3T3 cells, competence factors such as 12-O-tetradecanoylphorbol-13-acetate (TPA) and platelet-derived growth factor (PDGF) synergize with progression factors such as insulin to initiate DNA synthesis. In this study, we found that colchicine, a microtubule-disrupting agent, acted synergistically with TPA, but not with insulin, to induce the maximal stimulation of DNA synthesis. Colchicine also synergized with PDGF in the presence of epidermal growth factor to elicit nearly the optimal induction of DNA synthesis. Moreover, it acted synergistically with fibroblast growth factor, another competence factor. These results suggest that colchicine acts as a progression factor like insulin in quiescent Balb/c 3T3 cells.
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D003078:Colchicine / Q000494:pharmacology*; D004247:DNA / Q000096:biosynthesis*; D003703:Demecolcine / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005346:Fibroblast Growth Factors / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology; D007399:Interphase / Q000187:drug effects*; D008162:Lumicolchicines / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D008870:Microtubules / Q000187:drug effects*; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D007328:Insulin; D008162:Lumicolchicines; D010982:Platelet-Derived Growth Factor; C042109:lumicolchicine; D005346:Fibroblast Growth Factors; D004815:Epidermal Growth Factor; D004247:DNA; D013755:Tetradecanoylphorbol Acetate; D003078:Colchicine; D003703:Demecolcine