Partial characterization of antigens from M. leprae evoking IgG and IgM antibodies in armadillos.
Armadillo IgG and IgM antibody responses to Mycobacterium leprae were analyzed using isotypic-specific antisera by means of immunoblotting. Blots developed for IgG antibodies to M. leprae showed multiple protein antigens (Mr = 12-90 K) in some heavily infected armadillos. In contrast, blots developed for IgM antibodies to M. leprae showed a single, broad, diffuse band of immunoreactivity at approximately 33 kDa. The 33-kDa immunogen was detectable with silver stain modified for carbohydrate reactivity, suggesting the presence of a polysaccharide component. In addition, binding of 125I-concanavalin A to the 33-kDa component demonstrated the presence of mannose and/or glucose residues.
D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis*; D000942:Antigens, Bacterial / Q000032:analysis* / Q000276:immunology; D001135:Armadillos; D001426:Bacterial Proteins / Q000276:immunology; D002241:Carbohydrates / Q000276:immunology; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D007118:Immunoassay; D007074:Immunoglobulin G / Q000096:biosynthesis*; D007075:Immunoglobulin M / Q000096:biosynthesis*; D009166:Mycobacterium leprae / Q000276:immunology*
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D002241:Carbohydrates; D007074:Immunoglobulin G; D007075:Immunoglobulin M