Alternative splicing of SV40 early pre-mRNA in vitro.
Simian virus 40 (SV40) early pre-mRNA is spliced using either of two alternative 5' splice sites and a common 3' splice site to produce two mRNAs that encode the T and t antigens. We have studied alternative splicing of SV40 early pre-mRNA in vitro using a HeLa cell nuclear extract. Synthetic SV40 early transcripts are processed to T and t antigen mRNAs in vitro. As in SV40-infected cells in vivo, cleavage at the T antigen 5' splice site is more efficient than cleavage at the t antigen 5' splice site in vitro, although both of these 5' splice sites are utilized relatively inefficiently in vitro. The ratio of cleavage at the T and t antigen 5' splice sites is not changed significantly by a number of alterations in the conditions under which the in vitro splicing reactions are carried out.
D000818:Animals; D000952:Antigens, Polyomavirus Transforming; D000957:Antigens, Viral, Tumor / Q000235:genetics*; D002467:Cell Nucleus / Q000378:metabolism; D005260:Female; D005796:Genes; D005814:Genes, Viral; D006367:HeLa Cells / Q000378:metabolism; D006801:Humans; D009694:Nucleic Acid Precursors / Q000235:genetics*; D009856:Oncogene Proteins, Viral / Q000235:genetics*; D009865:Oocytes / Q000378:metabolism; D012322:RNA Precursors; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics*; D013539:Simian virus 40 / Q000235:genetics*; D014158:Transcription, Genetic; D014981:Xenopus
D000952:Antigens, Polyomavirus Transforming; D000957:Antigens, Viral, Tumor; D009694:Nucleic Acid Precursors; D009856:Oncogene Proteins, Viral; D012322:RNA Precursors; D012333:RNA, Messenger