The complete coding sequence of the human A-raf-1 oncogene and transforming activity of a human A-raf carrying retrovirus.
The complete 606 amino acid sequence of the human A-raf oncogene has been deduced from the 2453 nucleotide sequence of a human T cell cDNA. A cysteine-rich region located near the amino terminus, which is highly conserved in A-raf and c-raf, shows significant homology with protein kinase C. A 5' deleted fragment of the cDNA has been incorporated into a murine retrovirus which endows the virus with the ability to transform cells in vivo and in vitro. Functionally, human A-raf is similar to v-raf and v-mos in that transformation is independent of ras gene function.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002471:Cell Transformation, Neoplastic; D004247:DNA / Q000032:analysis; D005814:Genes, Viral; D006801:Humans; D008099:Liver / Q000196:embryology; D051379:Mice; D008980:Moloney murine sarcoma virus / Q000235:genetics*; D009857:Oncogenes; D009053:Sarcoma Viruses, Murine / Q000235:genetics*; D013601:T-Lymphocytes / Q000032:analysis
D004247:DNA