In-vitro modulation of protein kinase C activity by environmental chemical pollutants.
A number of environmental chemical pollutants have been reported to cause tumors or help in the propagation of tumors in experimental animals. The in-vitro effects of a few chemical contaminants were studied on the histone phosphorylation and 3H Phorbol dibutyrate (PdBu) binding of partially purified Ca2+/phospholipid dependent protein kinase c (PKC) from the brains of Fischer F344 and B6C3F1 mice. The enzyme was prepared by a modified method which gave approximately 75-fold purification. A differential effect of various compounds was observed on the phosphorylation activity and PdBu binding of PKC from rats and mice. The reported tumor promoting ability and effect on protein kinase C activity appeared to be related in the case of the rat enzyme, although causality cannot be inferred.
D000818:Animals; D004051:Diethylhexyl Phthalate / Q000633:toxicity; D004785:Environmental Pollutants / Q000633:toxicity*; D006657:Histones / Q000378:metabolism; D051379:Mice; D008815:Mice, Inbred Strains; D015240:Phorbol 12,13-Dibutyrate; D010703:Phorbol Esters / Q000378:metabolism; D010766:Phosphorylation; D011075:Polybrominated Biphenyls / Q000633:toxicity; D011078:Polychlorinated Biphenyls / Q000633:toxicity; D011493:Protein Kinase C / Q000032:analysis*; D051381:Rats; D011916:Rats, Inbred F344
D004785:Environmental Pollutants; D006657:Histones; D010703:Phorbol Esters; D011075:Polybrominated Biphenyls; D015240:Phorbol 12,13-Dibutyrate; D004051:Diethylhexyl Phthalate; D011078:Polychlorinated Biphenyls; D011493:Protein Kinase C