Transport of lactate and other short-chain monocarboxylates in the yeast Saccharomyces cerevisiae.
Saccharomyces cerevisiae IGC4072 grown in lactic acid medium transported lactate by an accumulative electroneutral proton-lactate symport with a proton-lactate stoichiometry of 1:1. The accumulation ratio measured with propionate increased with decreasing pH from ca. 24-fold at pH 6.0 to ca. 1,400-fold at pH 3.0. The symport accepted the following monocarboxylates (Km values at 25 degrees C and pH 5.5): D-lactate (0.13 mM), L-lactate (0.13 mM), pyruvate (0.34 mM), propionate (0.09 mM), and acetate (0.05 mM), whereas apparently a different proton symport accepted formate (0.13 mM). The lactate system was inducible and was subject to glucose repression. Undissociated lactic acid entered the cells by simple diffusion. The permeability of the plasma membrane for undissociated lactic acid increased exponentially with pH, and the diffusion constant increased 40-fold when the pH was increased from 3.0 to 6.0.
D000085:Acetates / Q000378:metabolism; D001692:Biological Transport; D002462:Cell Membrane / Q000378:metabolism; D002463:Cell Membrane Permeability; D005561:Formates / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D007773:Lactates / Q000378:metabolism*; D011422:Propionates / Q000378:metabolism; D011522:Protons; D011773:Pyruvates / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism*
D000085:Acetates; D005561:Formates; D007773:Lactates; D011422:Propionates; D011522:Protons; D011773:Pyruvates