Molecular pathogenesis of equine coital exanthema (ECE): temperature sensitivity (TS) and restriction endonuclease (RE) fragment profiles of several field isolates.
Examination of six field isolates of equine herpesvirus 3, the causative agent of equine coital exanthema, indicates that all were temperature sensitive (ts) at the body temperature, 39 degrees C, of their host (Equine asinus and callabus) when grown in cell culture. The isolates were characterized by fingerprint analysis with the restriction endonucleases XbaI, EcoRI, BamHI and Hind III to establish possible epidemiologic relatedness. Three of the six isolates may be considered related. Variation in the mobility of the BamHI-A and Hind III-K fragments indicates that a small plaque isolate may contain a 5.7 kb insert of DNA in the unique short region of the genome.
D000818:Animals; D002478:Cells, Cultured; D003588:Cytopathogenic Effect, Viral; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000032:analysis*; D005814:Genes, Viral; D006564:Herpesviridae / Q000254:growth & development*; D004858:Herpesvirus 3, Equid / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification; D006736:Horses; D009710:Nucleotide Mapping; D013696:Temperature
D004279:DNA, Viral; D004262:DNA Restriction Enzymes