Rapid simultaneous assessment of neutrophil superoxide generation and lysosomal enzyme release.
A rapid method for the simultaneous measurement of neutrophil superoxide generation and beta-glucuronidase release is described. Assay of beta-glucuronidase using a fluorescent substrate is shown to be valid in the presence of reduced or unreduced ferricytochrome C, a prerequisite for the simultaneous assessment of this enzyme activity and O-2 generation.
D003574:Cytochrome c Group / Q000494:pharmacology; D005966:Glucuronidase / Q000032:analysis*; D006801:Humans; D007700:Kinetics; D008247:Lysosomes / Q000201:enzymology*; D009504:Neutrophils / Q000378:metabolism*; D013481:Superoxides / Q000378:metabolism*
D003574:Cytochrome c Group; D013481:Superoxides; D005966:Glucuronidase