[Effect of mutations of Escherichia coli K-12 chromosome on the integration of bacteriophage Mu introduced into cells by infection or conjugation].
We present the detailed research on the previously described Escherichia coli K-12 Mud- mutants with impaired development of bacteriophage Mu. The ability of Mu phage DNA to penetrate into mutant cells on infection was shown. If introduced into the cells or combined with mud mutation by recombination, the prophage may be induced, which results in phage Mu lythic development and phage burst from mutant cells. In the course of conjugative transfer into the mutant cells, within a DNA fragment of the lysogenic donor chromosome, MupAp1 prophage is not inherited by recombinants. At the same time, Mu prophage deficient in genes A and B, whose products are required for transposition, is inherited by the mutant with the usual frequency. These data enable us to conclude that the mud mutations disturb the stage of conservative transposition which is connected with the insertion of the Mu prophage into the chromosome, after excision from the linear DNA introduced into the cells via infection or conjugation.
D010583:Bacteriophage mu / Q000235:genetics*; D003227:Conjugation, Genetic; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics*; D005819:Genetic Markers; D008242:Lysogeny; D009154:Mutation; D010957:Plasmids; D014162:Transfection
D004251:DNA Transposable Elements; D005819:Genetic Markers
Vliianie mutatsiĭ Escherichia coli K-12 na vnedrenie v khromosomu genoma bakteriofaga Mu, vnesennogo v kletki pri infektsii ili kon''iugatsii.