Structure determination of five sulfated oligosaccharides derived from tracheobronchial mucus glycoproteins.
The structure of five sulfated oligosaccharide units of highly anionic tracheobronchial mucous glycoproteins, isolated from a cystic fibrosis patient's sputum, were established. Reduced oligosaccharides (84%) were released under alkaline borohydride conditions, and the acidic oligosaccharides (63%) were isolated by Dowex 1-X2 chromatography. Following the removal of acidic oligosaccharides possessing N-acetylneuraminic acid and L-fucose by lectin affinity chromatography a heterogeneous mixture of sulfated oligosaccharides was obtained. From this fraction, five short chain monosulfated oligosaccharides (S-I to S-V) were purified by sequential separation by SynChroprep AX300 anion exchange high pressure liquid chromatography, gel filtration on Bio-Gel P-2, and high voltage paper electrophoresis. Based on the results of carbohydrate composition, sequential exoglycosidase degradation, permethylation analysis, lectin affinity chromatography, and periodate oxidation, the following structures (where GalNAcol is N-acetylgalactosaminitol) were proposed for these oligosaccharides. (formula; see text)
D000293:Adolescent; D001980:Bronchi / Q000378:metabolism*; D002236:Carbohydrate Conformation; D002241:Carbohydrates / Q000032:analysis; D002845:Chromatography; D003550:Cystic Fibrosis / Q000378:metabolism; D006023:Glycoproteins / Q000032:analysis* / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008297:Male; D008745:Methylation; D009093:Mucus / Q000032:analysis* / Q000378:metabolism; D009844:Oligosaccharides / Q000302:isolation & purification*; D010504:Periodic Acid; D013431:Sulfates / Q000378:metabolism*; D014132:Trachea / Q000378:metabolism*
D002241:Carbohydrates; D006023:Glycoproteins; D009844:Oligosaccharides; D013431:Sulfates; D010504:Periodic Acid; D006026:Glycoside Hydrolases