title: Production of the constant domain of murine T-cell receptor beta-chain in Escherichia coli.
abstract: T-cell antigen receptor is a heterodimer of disulfide-linked alpha- and beta-chains. Although the essential features of T-cell receptor seem to be rather similar to those of immunoglobulin, the amount of T-cell receptor expressed on the surface of a T-cell is not large enough to be analyzed physcio-chemically. In this study, the DNA fragment encoding 120 amino acids from the 116th to the 235th of the murine beta-chain which corresponds to the presumed constant domain was inserted into an expression vector in E. coli. A large amount of this 18 kDa protein was observed to be synthesized in E. coli, and might be a good source for the three dimensional analysis of the T-cell receptor molecule.
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004262:DNA Restriction Enzymes; D004926:Escherichia coli / Q000235:genetics*; D005796:Genes; D051379:Mice; D011401:Promoter Regions, Genetic; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D011948:Receptors, Antigen, T-Cell; D004262:DNA Restriction Enzymes