Long term liver preservation using artificial blood substitutes.
Twenty-four hour hypothermic perfusion preservation of the liver was aimed using an artificial blood substitutes. A canine liver was harvested and preserved using originally designed perfusates. In group 1, Perfluorotributylamine solution (Oxypherol solution) was used. In group 2, (Pyridoxylated hemoglobin)-(Polyoxyethylene) conjugate solution (Stabilized Hemoglobin, PHP-1). In group 3 and in group 4, modified Stabilized hemoglobin, PHP-3 and PHP-4 was used. After the preservation, each liver was transplanted orthotopically. Postoperative graft function was estimated the following parameter, such as bile excretion, consciousness level, activated clotting time, and survival rate. In group 1 and group 2, improvement of such factors were not fully observed. On the contrary, in group 3 and 4, complete recovery of the function was seen and in group 4, longer survival was obtained. PHP solution was considered suitable perfusate for liver preservation.
D000818:Animals; D001646:Bile / Q000378:metabolism; D001777:Blood Coagulation; D001802:Blood Substitutes; D004285:Dogs; D005466:Fluorocarbons; D006085:Graft Survival; D006454:Hemoglobins; D008099:Liver / Q000502:physiology; D016031:Liver Transplantation; D010101:Oxygen Consumption; D010477:Perfusion; D011092:Polyethylene Glycols; D012996:Solutions; D014021:Tissue Preservation / Q000379:methods*
D001802:Blood Substitutes; D005466:Fluorocarbons; D006454:Hemoglobins; D012996:Solutions; C050907:pyridoxalated-hemoglobin-polyethylene glycol conjugate; C008807:perfluorotributylamine; D011092:Polyethylene Glycols