Age-dependent changes in rat brain mitochondria of synaptic and non-synaptic origins.
Synaptic and non-synaptic mitochondria were isolated from the brains of 3- 12-and 28-30-month-old female Fisher 344 rats. Total oxygen consumption and oxygen consumption due to mitochondrial respiration decreases 83% with increasing age in synaptic mitochondria using malate plus glutamate as substrate, but only 33% in non-synaptic mitochondria; succinate-driven activity is not affected. Succinate-driven superoxide generation decreases over 90% in both fractions; malate plus glutamate-driven superoxide generation decreases 50% in synaptic mitochondria only. The amount of c- and a-type cytochromes decreases approximately 50% in synaptic mitochondria. The absorbance wavelength maximum of cytochrome b decreases 2.6 nm in synaptic mitochondria from senescent brains but only 1.6 nm in non-synaptic mitochondria.
D000818:Animals; D001921:Brain / Q000254:growth & development* / Q000378:metabolism; D003580:Cytochromes / Q000378:metabolism; D005260:Female; D005971:Glutamates / Q000378:metabolism; D008293:Malates / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism*; D010101:Oxygen Consumption; D051381:Rats; D011916:Rats, Inbred F344; D013053:Spectrophotometry; D013386:Succinates / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism; D013569:Synapses / Q000378:metabolism*
D003580:Cytochromes; D005971:Glutamates; D008293:Malates; D013386:Succinates; D013481:Superoxides