Radioimmunoassay of urinary 3 beta-hydroxy-5-cholenoyl glycine in hepatobiliary disease.
A radioimmunoassay for 3 beta-hydroxy-5-cholenoyl glycine in human urine has been developed. The antiserum was elicited with the antigen in which the steroid hapten is linked to a bovine serum albumin through the C-19 position. The [125I]-tyrosine derivative of the hapten was used as radioligand. The standard curves were linear ranging from 10 to 320 ng/mL. The cross-reactivities with other bile acids were not detectable and below 0.3% with cholesterol. Sample preparation includes extraction of 3 beta-hydroxy-5-cholenoyl glycine from urine and solvolysis of the sulfates--main form present in urine. Urinary excretion of 3 beta-hydroxy-5-cholenoyl glycine was 0.373 +/- 0.133 mumol/day in healthy adults. Urinary excretion of 3 beta-hydroxy-5-cholenoyl glycine increased in chronic liver dysfunction, hepatoma and obstructive jaundice in this order.
D000328:Adult; D000368:Aged; D001660:Biliary Tract Diseases / Q000652:urine*; D006528:Carcinoma, Hepatocellular / Q000652:urine; D002779:Cholestasis / Q000652:urine; D005260:Female; D006000:Glycocholic Acid / Q000031:analogs & derivatives* / Q000652:urine; D006801:Humans; D008107:Liver Diseases / Q000652:urine*; D008113:Liver Neoplasms / Q000652:urine; D008297:Male; D008875:Middle Aged; D011863:Radioimmunoassay
C051955:3-hydroxy-5-cholenoylglycine; D006000:Glycocholic Acid