[Growth of neurites and formation of connections in cultures of pteropodial mollusc neurons].
Dissociated neurons from the brain of pteropodial mollusc were cultivated in a 25% Leibovitz medium containing 2% of calf serum. Neurite outgrowth was observed in 1-30% of the neurons. It was maximum during the first 3 days. Neurite length reached 300 microns. Membrane potential of neurons was 40-60 mV; they generated single spikes or bursts of impulses. Intercellular connections were tested on the 3-4th days in 70 pairs of neurons with neurites overlapped. Electrical connections between the cells were observed in 20% of the pairs tested, and in 6% of pairs stimulation of one of the neurons evoked an inhibitory postsynaptic potential in the other.
D000818:Animals; D002450:Cell Communication; D002478:Cells, Cultured; D005724:Ganglia / Q000166:cytology*; D008564:Membrane Potentials; D008974:Mollusca; D009473:Neuronal Plasticity; D013045:Species Specificity; D013569:Synapses / Q000502:physiology; D009435:Synaptic Transmission
Rost otroskov i obrazovanie sviazeĭ v kul'ture neĭronov krylonogogo molliuska.