Rapid procedure for preparation of macrophage plasma membrane.
This report describes a simple and efficient procedure for the isolation of plasma membrane from guinea pig peritoneal macrophages. The use of polycationic beads (Affi-gel 731 beads) facilitates rapid and high-clear separation of plasma membrane within 30 min. The final plasma membrane coated beads fraction has high specific activities of marker enzymes with little contamination with mitochondrial, lysosomal or cytoplasmal markers.
D000818:Animals; D002458:Cell Fractionation / Q000379:methods*; D002462:Cell Membrane / Q000201:enzymology / Q000648:ultrastructure*; D006168:Guinea Pigs; D008264:Macrophages / Q000648:ultrastructure*; D008297:Male; D008855:Microscopy, Electron, Scanning; D010529:Peritoneal Cavity / Q000166:cytology; D010741:Phospholipases A / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism
D010741:Phospholipases A; D000254:Sodium-Potassium-Exchanging ATPase