A murine hybridoma with large cytoplasmic inclusions of kappa light chains.
A murine hybridoma cell line has been established that consistently forms large cytoplasmic inclusions. These structures bind antibody specific for mouse kappa L chain when stained in situ. SDS-PAGE analysis of isolated inclusion bodies produce a single protein band of approximately 26,000 Mr that reacts with anti-kappa antibody when transferred to nitrocellulose. No carbohydrate was detected in association with the purified protein. These data are consistent with the intracellular retention and deposition of complete kappa L chain protein.
D000818:Animals; D002458:Cell Fractionation; D003593:Cytoplasm / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D006825:Hybridomas / Q000276:immunology* / Q000648:ultrastructure; D007145:Immunoglobulin kappa-Chains / Q000032:analysis*; D007159:Immunologic Tests; D002479:Inclusion Bodies / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C
D007145:Immunoglobulin kappa-Chains