[Fundamental model assays on chemical damages to ribonucleic acids caused by oxygen radicals in aqueous solution].
A partial decomposition of RNA molecules in Saccharomyces cerevisiae is caused by photochemically produced superoxide anion radical (O2-.). Hydroxyl radicals (OH.) lead to complete destruction of RNA. Such radicals as originating from peroxidase-hydrogen peroxide reaction cause a partial decomposition of RNA molecules. All RNA species are damaged to the same extent by radicals. Damages due to oxygen radicals can be prevented or reduced by strong radical captors (dimethyl sulfoxide, 1,4-diazabicyclo-(2,2,2)-octane or superoxide dismutase). All these reactions proceed in aqueous solution. Correlations with the use of radicals in radiotherapy are discussed.
D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D005609:Free Radicals; D006801:Humans; D006861:Hydrogen Peroxide; D006878:Hydroxides; D017665:Hydroxyl Radical; D008027:Light; D008956:Models, Chemical; D010544:Peroxidases; D010777:Photochemistry; D010879:Piperazines / Q000494:pharmacology; D012331:RNA, Fungal / Q000032:analysis / Q000528:radiation effects*; D012334:RNA, Neoplasm / Q000528:radiation effects; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D011878:Radiotherapy / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000528:radiation effects*; D012996:Solutions; D013482:Superoxide Dismutase / Q000494:pharmacology; D013481:Superoxides
D005609:Free Radicals; D006878:Hydroxides; D010879:Piperazines; D012331:RNA, Fungal; D012334:RNA, Neoplasm; D011837:Radiation-Protective Agents; D012996:Solutions; D013481:Superoxides; D017665:Hydroxyl Radical; D006861:Hydrogen Peroxide; D010544:Peroxidases; D013482:Superoxide Dismutase; C007306:triethylenediamine; D004121:Dimethyl Sulfoxide
Grundlegende Modellversuche über chemische Schädigungen von Ribonukleinsäuren durch Sauerstoffradikale in wässriger Lösung.