Cytopathology, plaque assay, and heat inactivation of hepatitis A virus strain HM175.
Hepatitis A virus (HAV) strain HM175 derived after repeated subculture of persistently infected B-SC-1 cells caused a specific cytopathic effect upon acute infection of B-SC-1 cells. The virus formed visible plaques on B-SC-1 cell monolayers after 9 to 14 days of incubation at 34 degrees C, and virus can therefore be titrated by plaque assay. Virus could be re-isolated from plaques of infected cells, which allows the clonal isolation of HAV variants. The stability of a plaque-purified variant of HAV at elevated temperatures exceeded that of poliovirus type 1, but this variant is less stable than previously reported strains of HAV.
D000818:Animals; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D035922:Hepatitis A Antibodies; D006508:Hepatitis Antibodies / Q000276:immunology; D006507:Hepatovirus / Q000254:growth & development / Q000276:immunology / Q000302:isolation & purification / Q000502:physiology*; D006358:Hot Temperature; D009500:Neutralization Tests; D010948:Viral Plaque Assay
D035922:Hepatitis A Antibodies; D006508:Hepatitis Antibodies