title: Cloning and characterization of the gene for Escherichia coli seryl-tRNA synthetase.
abstract: Seryl-tRNA synthetase is the gene product of the serS locus in Escherichia coli. Its gene has been cloned by complementation of a serS temperature sensitive mutant K28 with an E. coli gene bank DNA. The resulting clones overexpress seryl-tRNA synthetase by a factor greater than 50 and more than 6% of the total cellular protein corresponds to the enzyme. The DNA sequence of the complete coding region and the 5'- and 3' untranslated regions was determined. Protein sequence comparison of SerRS with all available aminoacyl-tRNA synthetase sequences revealed some regions of significant homology particularly with the isoleucyl- and phenylalanyl-tRNA synthetases from E. coli.
mesh_terms: D000595:Amino Acid Sequence; D000604:Amino Acyl-tRNA Synthetases / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005796:Genes; D005798:Genes, Bacterial; D007533:Isoleucine-tRNA Ligase / Q000235:genetics; D010652:Phenylalanine-tRNA Ligase / Q000235:genetics; D010957:Plasmids; D012689:Sequence Homology, Nucleic Acid; D012714:Serine-tRNA Ligase / Q000235:genetics*
chemical_list: D004262:DNA Restriction Enzymes; D000604:Amino Acyl-tRNA Synthetases; D012714:Serine-tRNA Ligase; D010652:Phenylalanine-tRNA Ligase; D007533:Isoleucine-tRNA Ligase