cAMP-dependent ACTH secretagogues facilitate corticotropin releasing activity of angiotensin II on rat anterior pituitary cells in vitro.
Both arginine vasopressin (AVP) and angiotensin II (AII) potentiate the corticotropin-releasing activity of CRF41 via a potentiation of CRF41-induced cAMP production. In perfused rat anterior pituitary cells, AII (10(-8) mol) showed a transitory 2-fold increase in its ACTH-releasing activity, when tested after application extract of rat stalk median eminence. In order to determine whether this facilitating effect on AII corticotropin-releasing activity occurred through cAMP-dependent mechanisms, the ACTH-releasing activity of AII was tested after stimulation with CRF41, AVP or forskolin, three secretagogues with known effects on cAMP production. When given 16 min after CRF41, 10 micrograms/l, AII (10(-8) mol) showed a significant increase (210%) in its ACTH-releasing activity, which returned to the normal level when AII-stimulation was repeated at 32 min (121%) and 48 min (100%). Similarly, forskolin, 3 X 10(-6) mol, produced a significant transitory increase (208%) in the subsequent AII-induced ACTH release whereas AVP, 10 micrograms/l and 100 micrograms/l, had no effect on the following ACTH response to AII. These results suggest that the AII-induced ACTH secretion--which is cAMP independent--nevertheless may be modulated by previously stimulation of the cAMP pathway.
D000324:Adrenocorticotropic Hormone / Q000378:metabolism*; D000804:Angiotensin II / Q000494:pharmacology*; D000818:Animals; D001127:Arginine Vasopressin / Q000494:pharmacology*; D001681:Biological Assay; D003346:Corticotropin-Releasing Hormone / Q000378:metabolism / Q000494:pharmacology*; D000242:Cyclic AMP / Q000096:biosynthesis*; D005260:Female; D008473:Median Eminence / Q000032:analysis; D010903:Pituitary Gland, Anterior / Q000187:drug effects*; D051381:Rats; D011919:Rats, Inbred Strains; D014020:Tissue Extracts / Q000008:administration & dosage
D014020:Tissue Extracts; D000804:Angiotensin II; D001127:Arginine Vasopressin; D000324:Adrenocorticotropic Hormone; D003346:Corticotropin-Releasing Hormone; D000242:Cyclic AMP