[Cloning of mRNA nucleotide sequences amplified during DNA replication in the regenerating liver].
A library of double-stranded cDNA has been constructed using the mRNA of regenerating rat liver 20 hr after partial hepatectomy. The differential screening of the library with the regenerating liver specific and the resting-liver-specific single-stranded cDNA probes has identified 11 cDNA clones which sequences are preferentially expressed in regenerating rat liver. The RNA dot blot hybridization has shown that levels of RNA complementary to these clones are 3 to 8-fold higher in dividing cells as compared with resting cells.
D000818:Animals; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004261:DNA Replication; D004262:DNA Restriction Enzymes; D008115:Liver Regeneration; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000235:genetics*; D051381:Rats
D012333:RNA, Messenger; D004247:DNA; D004262:DNA Restriction Enzymes
Klonirovanie nukleotidnykh posledovatel'nosteĭ mRNK, soderzhanie kotorykh povysheno v period replikatsii DNK v regeneriruiushcheĭ pecheni.