Cyclic AMP induces a transient alkalinization in Dictyostelium.
In a wide range of cell types, stimulus-response coupling is accompanied by a rise in cytoplasmic pH (pHi). It is shown that stimulation of developing Dictyostelium discoideum cells with the chemoattractant cAMP also results in a rise in pHi. About 1.5 min after stimulation, pHi starts increasing from pHi approximately 7.45 to pHi approximately 7.60, as is revealed independently by two different pH null-point methods. The rise in pHi is transient, also with a persistent stimulus, and effectively inhibited by diethylstilbestrol (DES), strongly suggesting that the rise in pHi is accomplished by the DES-sensitive plasma membrane proton pump which has been demonstrated in D. discoideum.
D002454:Cell Differentiation; D002633:Chemotaxis; D000242:Cyclic AMP / Q000031:analogs & derivatives / Q000494:pharmacology / Q000502:physiology*; D003593:Cytoplasm / Q000502:physiology; D004023:Dictyostelium / Q000502:physiology*; D006863:Hydrogen-Ion Concentration; D013873:Thionucleotides / Q000494:pharmacology; D013997:Time Factors
D013873:Thionucleotides; C016957:adenosine-3',5'-cyclic phosphorothioate; D000242:Cyclic AMP