Relationship between renin mRNA and renin secretion in adrenalectomized, salt-depleted, or converting enzyme inhibitor-treated rats.
Renin secretion was stimulated in rats by adrenalectomy (ADX), by treatment with the converting enzyme inhibitor enalapril (MK 421), or by feeding a low salt diet plus furosemide treatment (FUR). Relative amounts of renin mRNA were measured by densitometric Northern blot analysis using a 32P-labeled 700 bp mouse submaxillary gland cDNA fragment as hybridization probe. Renin secretion was measured in isolated kidneys taken from pretreated rats. Treatment resulted in a 4.4-, 7- and 12.7-fold increase in total renin mRNA in kidneys of ADX, MK 421 and FUR rats, respectively. Plasma renin levels increased 5-, 9- and 12-fold in ADX, MK 421 and FUR rats, whereas ex vivo secretion rates from the isolated kidney were 4.7-, 0.5- and 4.2-fold, respectively, that of controls. Thus, a close relationship between increases in renin secretion in vivo (reflected by plasma renin concentration) and renin mRNA exists, whereas secretion rates in the isolated perfused kidney ex vivo change in a non-proportional fashion.
D000315:Adrenalectomy; D000818:Animals; D003101:Collodion; D003720:Densitometry; D004039:Diet, Sodium-Restricted; D004587:Electrophoresis, Agar Gel; D004656:Enalapril / Q000494:pharmacology; D005665:Furosemide / Q000494:pharmacology; D007668:Kidney / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D012083:Renin / Q000097:blood / Q000235:genetics / Q000378:metabolism*
D012333:RNA, Messenger; D004656:Enalapril; D005665:Furosemide; D003101:Collodion; D012083:Renin