title: Influence of cations on enamel phosphatase activity.
abstract: ATP and 5-Br-4-Cl-indoxyl phosphate were used as substrates for spectrophotometric measurement of phosphatase activity in partly mineralized bovine enamel. MgCl2, CaCl2, NaCl, and KCl were used as moderators of the enzyme activities. It is concluded that at least two phosphatases, active at alkaline pH, are present in the immature enamel matrix.
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D003743:Dental Enamel / Q000201:enzymology*; D008274:Magnesium / Q000494:pharmacology; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*; D011188:Potassium / Q000494:pharmacology; D051381:Rats; D012964:Sodium / Q000494:pharmacology
chemical_list: D012964:Sodium; D000469:Alkaline Phosphatase; D010744:Phosphoric Monoester Hydrolases; D000252:Calcium-Transporting ATPases; D008274:Magnesium; D011188:Potassium; D002118:Calcium