Isolation and characterization of phosphonoacetic acid-resistant mutants of human cytomegalovirus.
Mutants of the human cytomegalovirus (HCMV) that were 6- to 13-fold more resistant to phosphonoacetic acid than the wild-type HCMV (Towne) were isolated. Extracts from mycoplasma-free, mutant-infected cells had phosphonoacetate-resistant DNA polymerase activity in vitro. This strongly suggests that the selected mutations are in the HCMV DNA polymerase genes of these viruses.
D000645:Ammonium Sulfate / Q000494:pharmacology; D003587:Cytomegalovirus / Q000187:drug effects / Q000235:genetics*; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D004352:Drug Resistance, Microbial; D006801:Humans; D009154:Mutation; D009943:Organophosphorus Compounds / Q000494:pharmacology*; D010746:Phosphonoacetic Acid / Q000494:pharmacology*; D013045:Species Specificity
D009943:Organophosphorus Compounds; D004259:DNA-Directed DNA Polymerase; D010746:Phosphonoacetic Acid; D000645:Ammonium Sulfate