Cross-hybridisation of human papillomavirus DNA on filters.
Accurate type assignment of the different HPV types which infect the female genital tract, is essential in view of the differing pathological potential of the common virus types present in the cervix. We have developed hybridisation, washing and autoradiography conditions that minimise cross-hybridisation on filters and so allow clear-cut type assignment. We describe the conditions in this paper and have used this method to screen for HPV infection in clinical populations.
D001345:Autoradiography; D002584:Cervix Uteri / Q000382:microbiology; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D006801:Humans; D009693:Nucleic Acid Hybridization; D027383:Papillomaviridae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D014412:Tumor Virus Infections / Q000382:microbiology; D002577:Uterine Cervical Diseases / Q000382:microbiology
D004279:DNA, Viral