Sequences involved in accurate and efficient transcription of human c-myc genes microinjected into frog oocytes.
By microinjecting a series of deletion mutant constructs into Xenopus laevis oocytes, transcriptional control regions, two promoters, of the human c-myc gene were defined. In the case of the first promoter, sequences between -60 and -37 relative to the transcription start site contained an element essential for promoter activity. In the case of the second promoter, sequences between -66 and -56 relative to the initiation site appeared to be involved in accurate and efficient transcription. In both cases, the region identified as the essential promoter element contained GGGCGG or GGCGGG,GC box-like sequences, suggesting that c-myc gene promoter activity may be controlled by transcription factor Sp1 binding in the microinjected oocytes.
D000818:Animals; D002872:Chromosome Deletion; D004262:DNA Restriction Enzymes; D005260:Female; D006801:Humans; D008845:Microinjections; D009154:Mutation; D009857:Oncogenes; D009865:Oocytes / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic; D014981:Xenopus
D004262:DNA Restriction Enzymes