Localization of the mcf.2 transforming sequence to the X chromosome.
A transforming sequence was identified using co-transfection of DNA from the human mammary carcinoma cell line MCF-7 and of a G418 resistance gene into NIH 3T3 cells, followed by tumor formation in athymic mice. This sequence, named mcf.2, was molecularly cloned. A transforming activity resides in a cosmid clone of 42 kb. mcf.2 did not cross-hybridize with the known oncogenes tested. In situ hybridization localized it on the X chromosome, probably at q27. This localization was confirmed by hybridization to a panel of human--rodent cell line DNAs.
D000818:Animals; D001943:Breast Neoplasms; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003360:Cosmids; D004262:DNA Restriction Enzymes; D005260:Female; D006801:Humans; D006822:Hybrid Cells / Q000166:cytology; D009693:Nucleic Acid Hybridization; D012334:RNA, Neoplasm / Q000235:genetics; D014960:X Chromosome
D012334:RNA, Neoplasm; D004262:DNA Restriction Enzymes