Activity and stability of recombinant human superoxide dismutase in buffer solutions and hypothermic perfusates.
The stability of recombinant human superoxide dismutase (r-hSOD) in buffer solutions was studied in solutions at various pH and temperatures. Additionally, we studied the effects of incubation with proteases, serum and two types of hypothermic perfusates. R-hSOD was stable in the pH range of 6-11 and at temperatures up to 80 degrees C for 30 min. R-hSOD activity was not affected by incubation with trypsin, aminopeptidase M or serum for 2 h. R-hSOD activity determined at various temperatures (4-37 degrees C) did not vary remarkably. R-hSOD in hypothermic perfusates was stable at 4-37 degrees C for 24 h.
D002021:Buffers; D004795:Enzyme Stability; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010447:Peptide Hydrolases / Q000494:pharmacology; D010477:Perfusion; D011994:Recombinant Proteins / Q000032:analysis; D013482:Superoxide Dismutase / Q000032:analysis*; D013696:Temperature
D002021:Buffers; D011994:Recombinant Proteins; D013482:Superoxide Dismutase; D010447:Peptide Hydrolases