In vivo studies of repair of 2-aminopurine in Escherichia coli.
The repair of the base analog 2-aminopurine has been studied in vivo by using a temperature-sensitive mutant of the cloned mutH gene of Escherichia coli. Our results suggest that the lethal event in killing of dam mutants by 2-aminopurine does not result simply from incorporation of 2-aminopurine into the DNA and its subsequent repair. Furthermore, a 10-fold increase in the level of 2-aminopurine incorporated into the DNA of a dam mutH double mutant has little effect on the mutation frequency of this strain. An alternative mechanism for the mutagenicity of 2-aminopurine in E. coli is proposed.
D015075:2-Aminopurine / Q000378:metabolism*; D000225:Adenine / Q000031:analogs & derivatives*; D004260:DNA Repair; D004269:DNA, Bacterial / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D005798:Genes, Bacterial; D008780:Methyltransferases / Q000235:genetics; D009154:Mutation; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific); D013696:Temperature
D004269:DNA, Bacterial; D015075:2-Aminopurine; D008780:Methyltransferases; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific); D000225:Adenine