Effect of irradiation and mutagenic chemicals on the generation of ADH2-constitutive mutants in yeast. Significance for the inducibility of Ty transposition.
Mutations caused by the insertion of a Ty element resulting in an antimycin-A-resistant phenotype in an adh1- strain of Saccharomyces cerevisiae were used as an assay for the quantitative detection of Ty transposition. Antimycin-A-resistant mutants were found to be inducible by ethyl methanesulfonate (EMS) as well as by gamma- and UV irradiation. DNA analysis of gamma-induced mutants showed an increase of the fraction of Ty insertions in the ADH2 locus with increasing dose.
D000426:Alcohol Dehydrogenase / Q000235:genetics*; D004249:DNA Damage; D004251:DNA Transposable Elements / Q000187:drug effects / Q000528:radiation effects; D004271:DNA, Fungal / Q000187:drug effects / Q000528:radiation effects; D005020:Ethyl Methanesulfonate / Q000494:pharmacology; D005656:Fungal Proteins / Q000235:genetics*; D005720:Gamma Rays; D005800:Genes, Fungal / Q000187:drug effects / Q000528:radiation effects; D007527:Isoenzymes / Q000235:genetics*; D009153:Mutagens / Q000494:pharmacology*; D011401:Promoter Regions, Genetic; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics* / Q000528:radiation effects; D014466:Ultraviolet Rays
D004251:DNA Transposable Elements; D004271:DNA, Fungal; D005656:Fungal Proteins; D007527:Isoenzymes; D009153:Mutagens; D005020:Ethyl Methanesulfonate; D000426:Alcohol Dehydrogenase