title: MAL64c is a global regulator of alpha-glucoside fermentation: identification of a new gene involved in melezitose fermentation.
abstract: Maltase constitutive mutants at the MAL6 locus have been mapped to the newly identified regulatory gene MAL64c. We show here that MAL64c has in addition pleiotropic effects on sugar fermentation: MAL64c strains constitutively synthesize an alpha-methylglucosidase and can complement a new gene, MTP1, for the fermentation of melezitose and alpha-methylglucoside. MTP1, maps near MAL1, and either encodes a permease which transports melezitose, alpha-methylglucoside, and maltose or regulates the activity of such a permease. This work shows that MAL64c, a trans-acting regulatory gene, is a global regulatory gene affecting several different pathways of alpha-glucoside metabolism.
mesh_terms: D000483:Alleles; D005285:Fermentation; D005796:Genes; D005800:Genes, Fungal; D005809:Genes, Regulator; D005838:Genotype; D008320:Maltose / Q000235:genetics; D008757:Methylglucosides / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D014312:Trisaccharides / Q000378:metabolism*; D000520:alpha-Glucosidases / Q000235:genetics / Q000378:metabolism
chemical_list: D008757:Methylglucosides; D014312:Trisaccharides; C027020:methylglucoside; D008320:Maltose; C034251:4-nitrophenyl-alpha-glucosidase; C034695:alpha-methylglucosidase; D000520:alpha-Glucosidases; C005190:melezitose