A simple method for the preparation of paraffin-embedded cell blocks from fine needle aspirates, effusions and brushings.
A simple method for the preparation of paraffin-embedded cell blocks from cytologic specimens obtained by fine needle aspiration, by brushing or from effusions is described. The cells are fixed in suspension in 50% ethanol for one hour and pelleted by centrifugation in a 50-mL plastic tube. The fixative is removed, and the pellet is suspended in 3 mL of acetone for dehydration for ten minutes and thereafter repelleted. The acetone is then removed, and the cell pellet is dried at 60 degrees C for one hour. Melted paraffin is added onto the dry warmed cell mass and allowed to solidify at room temperature. A conical paraffin block with the cells in the top is obtained and can be handled as a routine tissue block.
D001707:Biopsy, Needle; D003584:Cytological Techniques; D005122:Exudates and Transudates / Q000166:cytology*; D005404:Fixatives; D006801:Humans; D010232:Paraffin
D005404:Fixatives; D010232:Paraffin