Application of biotechnology methods to the study of cestodes.
The advent of biotechnology has invigorated research on the control of cestode diseases, especially cysticercosis infections in man and animals. The utilization of hybridoma technology to produce antigen-specific monoclonal antibodies has resulted in great strides towards obtaining pure antigens relevant for immunodiagnostic purposes and for research on vaccines. However, the isolation and identification of antigens is only the initial step in the development of such reagents. Production of antigens in quantities sufficient for research/development and commercialization is hampered by the scarcity of viable parasite material for extraction. Expectations are that this problem can be surmounted by application of recombinant DNA methods to produce cloned genes for antigen expression in cultured microorganisms or cells. Remarkable progress has been made recently in isolating and cloning genes from several important cestode species and antigens have been expressed in vitro with genes cloned from Taenia taeniaformis and T. hydatigena. Although these early efforts have not as yet resulted in practical antigen production, the prospects for doing so appear good. The complex epidemiology of cestode diseases is another research subject that has benefited from the successful application of the tools of biotechnology. For example, the greater precision in typing biological variants afforded by DNA analysis has led to important revisions of the understanding of hydatid disease. DNA probes are now available for Echinococcus spp, which are effective for typing isolates. These probes may also find use as reagents for distinguishing eggs of Echinococcus from other taeniid eggs, a serious difficulty for field investigations.(ABSTRACT TRUNCATED AT 250 WORDS)
D000818:Animals; D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth / Q000302:isolation & purification; D001709:Biotechnology; D002589:Cestoda / Q000276:immunology; D002590:Cestode Infections / Q000175:diagnosis*; D004274:DNA, Recombinant; D006801:Humans; D012698:Serologic Tests / Q000379:methods*
D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth; D004274:DNA, Recombinant