A possible mechanism of protection by polyamines against gastric damage induced by acidified ethanol in rats: polyamine protection may depend on its antiperoxidative properties.
The protective mechanism of polyamines against acidified ethanol-induced gastric damage was studied. Their oral administration prevented the formation of gastric mucosal lesions induced by 90% ethanol in 150 mM HCl in a dose-dependent manner, with the order of the protective potency being spermine greater than spermidine greater than putrescine. The acidified ethanol-induced lesions were accompanied by a concomitant increase in gastric mucosal lipid peroxide levels, but spermine in a protective dose could prevent the increment of lipid peroxides. Polyamines, in a concentration-dependent fashion, inhibited the reduction of nitroblue tetrazolium by superoxide anion radicals generated in vitro in the xanthine-xanthine oxidase system and the lipid peroxidation in vitro induced by ferrous ion in the porcine gastric mucosal homogenate. The order of the superoxide scavenging potency and the inhibitory potency of iron-induced lipid peroxidation by polyamines corresponded to the order to the protective potency against acidified ethanol-induced gastric lesions. The present results suggest that cytoprotection by polyamines may be responsible for their antiperoxidative activities.
D000284:Administration, Oral; D000818:Animals; D000431:Ethanol / Q000633:toxicity*; D005753:Gastric Mucosa / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D006851:Hydrochloric Acid; D007501:Iron / Q000494:pharmacology; D008054:Lipid Peroxides / Q000378:metabolism; D008297:Male; D011700:Putrescine / Q000008:administration & dosage*; D051381:Rats; D011919:Rats, Inbred Strains; D013095:Spermidine / Q000008:administration & dosage*; D013096:Spermine / Q000008:administration & dosage*; D013438:Sulfhydryl Compounds / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism; D013552:Swine; D013997:Time Factors
D008054:Lipid Peroxides; D013438:Sulfhydryl Compounds; D013481:Superoxides; D013096:Spermine; D000431:Ethanol; D007501:Iron; D006851:Hydrochloric Acid; D013095:Spermidine; D011700:Putrescine