Purification from a human hepatoma cell line of a basic fibroblast growth factor-like molecule that stimulates capillary endothelial cell plasminogen activator production, DNA synthesis, and migration.
A 17,500-dalton protein which stimulates plasminogen activator production in cultured bovine capillary endothelial cells has been purified from a SK-Hep-1 human hepatoma cell lysate by using heparin affinity chromatography and fast protein-liquid ion exchange chromatography. The purified molecule stimulated plasminogen activator production in a dose-dependent manner between 0.01 and 1 ng/ml. It also stimulated collagenase synthesis, DNA synthesis, and motility in capillary endothelial cells in the same concentration range. This molecule was identified as a basic fibroblast growth factor-like molecule on the basis of its biological activity, its affinity for heparin-Sepharose, and its cross-reactivity with a polyclonal antibody raised against the human placental basic fibroblast growth factor.
D000818:Animals; D002196:Capillaries / Q000187:drug effects / Q000502:physiology; D006528:Carcinoma, Hepatocellular / Q000503:physiopathology*; D002417:Cattle; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D004261:DNA Replication / Q000187:drug effects*; D004727:Endothelium / Q000187:drug effects / Q000502:physiology*; D005346:Fibroblast Growth Factors / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D007106:Immune Sera; D008113:Liver Neoplasms / Q000503:physiopathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D003012:Microbial Collagenase / Q000096:biosynthesis; D008970:Molecular Weight; D010959:Tissue Plasminogen Activator / Q000096:biosynthesis*; D014568:Urokinase-Type Plasminogen Activator / Q000096:biosynthesis
D007106:Immune Sera; D005346:Fibroblast Growth Factors; D010959:Tissue Plasminogen Activator; D014568:Urokinase-Type Plasminogen Activator; D003012:Microbial Collagenase