Perfused and ventilated guinea-pig lung: a method for evaluating xenobiotic effects on arachidonic acid using formaldehyde.
Lipoxygenase as well as cyclooxygenase pathways of arachidonic acid (AA) metabolism are involved in antigen-induced bronchoconstriction in ovalbumin-sensitized guinea-pig lungs. Chemical lung challenge induced by aerosol containing formaldehyde (5 ppm up to 20 ppm) was effective in increasing thromboxane (TX) B2 release in a dose-dependent manner, without affecting leukotriene (LT) release. This suggests that xenobiotics acting by non-immunologic mechanisms on bronchial mucosa may stimulate the cyclooxygenase pathway of AA metabolism, which, in turn, might cause bronchial and/or lung parenchymal effects of pathophysiological relevance.
D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism*; D005557:Formaldehyde / Q000633:toxicity*; D006168:Guinea Pigs; D066298:In Vitro Techniques; D007975:Leukotriene B4 / Q000378:metabolism; D008168:Lung / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D010477:Perfusion; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism; D013189:SRS-A / Q000378:metabolism; D013929:Thromboxane B2 / Q000378:metabolism
D001095:Arachidonic Acids; D013189:SRS-A; D005557:Formaldehyde; D007975:Leukotriene B4; D016718:Arachidonic Acid; D013929:Thromboxane B2; D011451:Prostaglandin-Endoperoxide Synthases