Affinity labeling of adenosine A1 binding sites.
The adenosine A1 receptors of sheep brain membranes have been identified by the specific binding of radiolabeled cyclohexyl[3H]adenosine ([3H]CHA). Pretreatment of membranes with periodate-oxidized CHA causes a dose- and time-dependent decrease in the number of binding sites. No decrease occurs when membranes are pretreated with CHA. Binding of [3H]CHA to the remaining sites occurs with the same characteristics as binding to the untreated receptor population.
D000241:Adenosine / Q000031:analogs & derivatives / Q000378:metabolism*; D000345:Affinity Labels / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D007700:Kinetics; D008566:Membranes / Q000378:metabolism; D010504:Periodic Acid / Q000494:pharmacology; D011983:Receptors, Purinergic / Q000378:metabolism*; D012756:Sheep
D000345:Affinity Labels; D011983:Receptors, Purinergic; D010504:Periodic Acid; C027513:N(6)-cyclohexyladenosine; D000241:Adenosine