Induction of c-Ha-ras transcription in rat cells by simian virus 40 large T antigen.
Rat 3Y1 cells expressing simian virus 40 large T antigen under the control of the mouse mammary tumor virus long terminal repeat were established. The amount of c-Ha-ras mRNA in those cells was elevated by about 20 times in parallel with large T antigen after exposure to dexamethasone for 48 h. In chloramphenicol acetyltransferase assays with a plasmid containing the c-Ha-ras-1 promoter the increase in c-Ha-ras mRNA was shown to occur at the transcriptional level.
D000818:Animals; D000952:Antigens, Polyomavirus Transforming; D000957:Antigens, Viral, Tumor / Q000235:genetics*; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D004262:DNA Restriction Enzymes; D003907:Dexamethasone / Q000494:pharmacology; D009856:Oncogene Proteins, Viral / Q000235:genetics*; D009857:Oncogenes; D011401:Promoter Regions, Genetic; D011494:Protein Kinases / Q000235:genetics*; D051381:Rats; D013539:Simian virus 40 / Q000201:enzymology / Q000235:genetics*; D014158:Transcription, Genetic / Q000187:drug effects
D000952:Antigens, Polyomavirus Transforming; D000957:Antigens, Viral, Tumor; D009856:Oncogene Proteins, Viral; D003907:Dexamethasone; D011494:Protein Kinases; D004262:DNA Restriction Enzymes