title: Phosphodiester bond cleavage outside mitochondria is required for the completion of protein import into the mitochondrial matrix.
abstract: The present studies show that hydrolysis of a phosphodiester bond, most likely ATP, is a distinct, second step required to complete import of the F1-ATPase beta-subunit into the mitochondria. This step follows a membrane potential-dependent first step. We show, using an inhibitor of adenine nucleotide transport and the analogue beta,gamma-AMP-PCP, that the activity required for this phosphodiester hydrolysis-dependent completion of protein import resides outside the mitochondrial inner membrane. This activity is proposed to act on the precursor at the site of translocation either to render it competent or to catalyze its vectorial movement directly through the import apparatus. This activity shares properties ascribed to proteins of the heat-shock family, which are proposed to participate in the ATP-dependent refolding of partially denatured proteins and nascent peptides.
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D007425:Intracellular Membranes / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism*; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism; D011506:Proteins / Q000378:metabolism; D011817:Rabbits
chemical_list: D011506:Proteins; D000255:Adenosine Triphosphate; D010727:Phosphoric Diester Hydrolases