[Dynamics of the accumulation of virus particles with different physico-chemical properties in FRhK-4 cells infected with hepatitis A virus].
The propagation time-course of hepatitis A virus (HAV, strain HAS-15) in continuous culture of the foetal rhesus monkey kidney cells (FRhK-4) was investigated. The HAV infectivity and viral RNA content in the infected cells reached the maximal level 5-8 days after infection, while accumulation of hepatitis A antigen (HAAg) continued for 2-3 weeks more. Viral particles with the densities 1.27-1.28 g/cm3 and 1.18-1.22 g/cm3 were isolated from the infected cells as well as the mature virions with the buoyant density 1.33-1.34 g/cm3 in CsCl. The concurrent accumulation of mature virus and "light" particles (1.18-1.22 g/cm3) was registered during infection. Viral particles with the density 1.27-1.28 g/cm3 accumulated predominantly from the 14th to the 21st-24th days after infection. The mature virions (1.34 g/cm3) as well as the particles with the density 1.24-1.25 g/cm3 were isolated from supernatant precipitated by ammonium sulphate. The HAAg activity of both fractions increased progressively in equal proportion in course of infection.
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002460:Cell Line; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D035921:Hepatitis A Antigens; D006507:Hepatovirus / Q000032:analysis / Q000276:immunology / Q000502:physiology*; D008253:Macaca mulatta; D014771:Virion / Q000032:analysis / Q000276:immunology / Q000502:physiology*; D014776:Virus Cultivation; D014779:Virus Replication
D000956:Antigens, Viral; D035921:Hepatitis A Antigens
Dinamika nakopleniia virusnykh chastits s razlichnymi fiziko-khimicheskimi svoĭstvami v infitsirovannykh virusom gepatita A v kletkakh FRhK-4.