Segregation of nucleosomes in replicated mouse alpha-globin gene.
DNA of mouse erythroleukemia cells grown in vitro was labeled with bromodeoxyuridine during cycloheximide-inhibited protein synthesis. Isolated nuclei were digested with micrococcal nuclease to obtain monosomes and monosomal dsDNA. The protection of the heavy and of the light strands of the newly replicated DNA was studied by dot hybridization with the coding and with its complementary noncoding strand of the alpha-globin gene. The results show that both sides of the replication fork contain protected sequences of the gene, thus supporting a bilateral (dispersive) mode of nucleosome segregation during DNA replication.
D000818:Animals; D001973:Bromodeoxyuridine; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D003513:Cycloheximide / Q000494:pharmacology; D004261:DNA Replication; D004262:DNA Restriction Enzymes; D004273:DNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D005796:Genes; D005914:Globins / Q000235:genetics*; D004915:Leukemia, Erythroblastic, Acute; D051379:Mice; D009693:Nucleic Acid Hybridization; D009707:Nucleosomes / Q000378:metabolism*
D004273:DNA, Neoplasm; D009707:Nucleosomes; D005914:Globins; D003513:Cycloheximide; D004262:DNA Restriction Enzymes; D001973:Bromodeoxyuridine