Differential response of the human hepatoma-derived cell line HA22T/VGH to polypeptide mitogens.
Several human cell lines derived from primary cancer of the liver are able to grow under serum-free conditions and produce spreading and growth factors which are released into the culture medium. Since this autocrine growth under hormone-free conditions might play a basic role in malignant transformation, we studied the effect on cell replication and the presence of specific membrane receptors of epidermal growth factor (EGF) and insulin on a dedifferentiated human hepatoma cell line, named HA22T/VGH. Our results point to a similar inhibitory effect on cell replication in the presence of both EGF and insulin, in spite of detecting different affinities of binding.
D006528:Carcinoma, Hepatocellular / Q000382:microbiology / Q000473:pathology*; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D004279:DNA, Viral / Q000032:analysis; D004815:Epidermal Growth Factor / Q000378:metabolism / Q000494:pharmacology*; D066246:ErbB Receptors / Q000378:metabolism; D013006:Growth Hormone / Q000494:pharmacology; D006514:Hepatitis B Surface Antigens / Q000235:genetics; D006515:Hepatitis B virus / Q000235:genetics; D006801:Humans; D007328:Insulin / Q000378:metabolism / Q000494:pharmacology*; D008113:Liver Neoplasms / Q000382:microbiology / Q000473:pathology*; D011972:Receptor, Insulin / Q000378:metabolism
D004279:DNA, Viral; D006514:Hepatitis B Surface Antigens; D007328:Insulin; D004815:Epidermal Growth Factor; D013006:Growth Hormone; D066246:ErbB Receptors; D011972:Receptor, Insulin