[Instability of immunoglobulin expression during differentiation induction in the human lymphoblastoid cell line RPMI-6410t].
The induction of immunoglobulin heavy chain classes switch from IgM to IgG was demonstrated in vitro in cells of RPMI-6410t line. The IgG+-sublines, formed as a result of the switch are characterized by instability of IgG synthesis. After removal of the inductor from the growth environment, IgG+ cells gradually reduce the level of secreted IgG. Such a transition to the functional rest state is likely to be connected with the convertible Ig-gene activity suppression in IgG+ cells, since after their activation by LPS IgG-secretion is partly or completely restored. The IgG+-sublines obtained may serve a convenient model for investigating the Ig-gene expression regulation in differentiated human B-cells.
D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D002478:Cells, Cultured; D004926:Escherichia coli; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D007134:Immunoglobulin Switch Region / Q000187:drug effects / Q000235:genetics; D007136:Immunoglobulins / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D012475:Salmonella; D014734:Vibrio cholerae
D007136:Immunoglobulins; D008070:Lipopolysaccharides
Nestabil'nost' ékspressii immunoglobulinov pri induktsii differentsirovki v linii limfoblastoidnykh kletok cheloveka RPMI-6410t.