title: Hypochlorite scavenging by Pseudomonas aeruginosa alginate.
abstract: Alginic acid was purified from a mucoid clinical isolate of Pseudomonas aeruginosa. Luminol-dependent chemiluminescence of phorbol myristate acetate-stimulated neutrophils was inhibited by this alginate, but oxygen consumption was unaffected. Further studies indicated that this effect was due to the ability of the pseudomonal alginate to scavenge hypochlorite. A seaweed alginate was less effective and dextran T500 was ineffective in hypochlorite scavenging. It appears that the uronic acid core and the O-acetyl groups of pseudomonal alginate are involved in its hypochlorite-scavenging ability. The relevance of this phenomenon was demonstrated by the greater resistance to killing by hypochlorite of mucoid P. aeruginosa compared with a nonmucoid revertant, and the addition of purified alginate to the nonmucoid revertant protected the organism from hypochlorite. Thus, this extracellular polysaccharide may enhance the virulence of P. aeruginosa by scavenging the phagocyte-generated oxidant HOCl. This enhanced virulence may be involved in disease processes in which mucoid organisms predominate, such as cystic fibrosis.
mesh_terms: D000464:Alginates / Q000378:metabolism*; D003550:Cystic Fibrosis / Q000382:microbiology; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D006997:Hypochlorous Acid / Q000378:metabolism*; D008163:Luminescent Measurements; D010101:Oxygen Consumption; D009195:Peroxidase / Q000378:metabolism; D010587:Phagocytosis; D011135:Polysaccharides, Bacterial / Q000502:physiology; D011550:Pseudomonas aeruginosa / Q000502:physiology*; D014783:Viscosity
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; D011135:Polysaccharides, Bacterial; D006997:Hypochlorous Acid; D020723:Glucuronic Acid; D006861:Hydrogen Peroxide; D009195:Peroxidase