[Use of the agar diffusion precipitation method for detecting rotavirus antigen].
The possibility of rotavirus detection in feces of patients with acute enteric diseases by the agar gel diffusion (AGD) test was studied. The effectiveness of this method was compared with that of the standard method, direct electron microscopy. Both methods showed good correlation of the results, but the AGD test is methodically much simpler which recommends it for diagnosis of rotavirus infection. Rotavirus-specific hyperimmune calf serum may by used as a serological diagnostic preparation for the detection of human rotavirus antigen.
D000208:Acute Disease; D000362:Agar; D000956:Antigens, Viral / Q000032:analysis*; D005243:Feces / Q000382:microbiology; D006801:Humans; D005779:Immunodiffusion / Q000379:methods; D007410:Intestinal Diseases / Q000175:diagnosis; D008854:Microscopy, Electron; D012401:Rotavirus / Q000276:immunology* / Q000648:ultrastructure; D012400:Rotavirus Infections / Q000175:diagnosis
D000956:Antigens, Viral; D000362:Agar
Ispol'zovanie metoda diffuzionnoĭ pretsipitatsii v agare dlia obnaruzheniia rotavirusnogo antigena.