Developmental changes in the chromatin of the brain of the rat: analysis by nick-translation.
Nick translation of nuclei of the brain of 3- 14- and 30-day old rats was carried out following their digestion by DNase I. The incorporation of 3H-dTMP at 14- and 30-day is significantly lower than at 3-day. This may be due to a lower proportion of active chromatin (DNase I hypersensitive sites) and condensation of chromatin with progressive development. When nuclei were digested by EcoRI and then nick-translated, the incorporation of 3H-dTMP showed the same pattern. Since the EcoRI sites are believed to be randomly distributed, the overall conformation of chromatin including the DNase I sensitive sites seems to undergo increasing compaction with development.
D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn / Q000502:physiology; D001921:Brain / Q000502:physiology*; D002455:Cell Division; D002467:Cell Nucleus / Q000378:metabolism; D002843:Chromatin / Q000502:physiology* / Q000648:ultrastructure; D004262:DNA Restriction Enzymes; D015246:Deoxyribonuclease EcoRI; D003850:Deoxyribonuclease I; D005786:Gene Expression Regulation; D066298:In Vitro Techniques; D008297:Male; D051381:Rats
D002843:Chromatin; D004262:DNA Restriction Enzymes; D015246:Deoxyribonuclease EcoRI; D003850:Deoxyribonuclease I