title: Direct effects of neutrophil oxidants on elastase-induced extracellular matrix proteolysis.
abstract: Oxidant species produced by human polymorphonuclear leukocytes (PMN) inactivate alpha-1-protease inhibitor and thus may indirectly enhance neutrophil elastase-induced proteolysis. It is unclear, however, if PMN-derived oxidants directly enhance proteolysis of extracellular matrix by neutrophil elastase. Matrix was produced by neonatal rat aortic smooth muscle cells and pulse-labeled with 3H-lysine to allow identification of the collagen-specific amino acid, hydroxylysine (3H-HL), and the elastin specific amino acid, desmosine (3H-DES). The smooth muscle cells were lysed, and the remaining matrix was used as a culture surface and a proteolytic substrate for intact PMN and purified neutrophil elastase. Proteolysis of collagen and elastin were quantified by chromatographic separation of the marker amino acids 3H-HL and 3H-DES, which were released into the supernatant or remained in the matrix after a 3-h incubation at 37 degrees C. The peptide, formyl-methionine-leucine-phenylalanine (FMLP), produced more rapid release of myeloperoxidase than did phorbol myristate acetate (PMA), which produced more release of O2- and H2O2 than did FMLP. The percent release of total matrix 3H-DES in the presence of PMN + FMLP was 2.45 +/- 0.19% (mean +/- SE, n = 6) and with PMN + PMA it was 1.32 +/- 0.1% (n = 6, p less than 0.01). The release of matrix 3H-HL did not differ. Neutrophil cytoplasts, which produced O-2 and H2O2 but lacked azurophilic granules, did not significantly enhance either elastin or collagen degradation by purified neutrophil elastase (NE).(ABSTRACT TRUNCATED AT 250 WORDS)
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000378:metabolism; D002448:Cell Adhesion; D003094:Collagen / Q000378:metabolism; D003594:Cytoplasmic Granules / Q000378:metabolism; D004549:Elastin / Q000378:metabolism; D005109:Extracellular Matrix / Q000201:enzymology*; D006801:Humans; D006861:Hydrogen Peroxide / Q000502:physiology*; D006868:Hydrolysis; D009504:Neutrophils / Q000201:enzymology*; D010100:Oxygen / Q000502:physiology*; D010196:Pancreatic Elastase / Q000378:metabolism*; D010447:Peptide Hydrolases / Q000378:metabolism; D009195:Peroxidase / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D013481:Superoxides / Q000378:metabolism; D000515:alpha 1-Antitrypsin
chemical_list: D001798:Blood Proteins; D000515:alpha 1-Antitrypsin; D013481:Superoxides; D003094:Collagen; D004549:Elastin; D006861:Hydrogen Peroxide; D009195:Peroxidase; D010447:Peptide Hydrolases; D010196:Pancreatic Elastase; D010100:Oxygen