Amplification of IMR-32 clones 8, G21, and N-myc in human neuroblastoma xenografts.
Amplification of clones 8, G21, and N-myc, which were derived from human neuroblastoma cell lines IMR-32 and NB-19, were studied in nine neuroblastoma xenografts. N-myc was amplified from 50- to 120-fold in eight of nine xenografts, clone 8 was amplified in five of the xenografts, and clone G21 was amplified in four of these five. Each of these clones was localized by in situ hybridization to homogeneously staining regions in metaphase spreads of xenograft chromosomes. In one xenograft a DNA rearrangement of clone 8 was observed, and only two of the sequences detected by G21 were amplified. Restriction enzyme mapping indicated that the rearrangement within clone 8 occurred at a position close to the rearrangement previously noted in neuroblastoma cell line NB-9.
D002460:Cell Line; D004262:DNA Restriction Enzymes / Q000378:metabolism; D015246:Deoxyribonuclease EcoRI; D015247:Deoxyribonuclease HindIII; D015252:Deoxyribonucleases, Type II Site-Specific; D005784:Gene Amplification; D006801:Humans; D007621:Karyotyping; D009368:Neoplasm Transplantation; D009447:Neuroblastoma / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D014183:Transplantation, Heterologous
D004262:DNA Restriction Enzymes; D015246:Deoxyribonuclease EcoRI; D015247:Deoxyribonuclease HindIII; C024408:endodeoxyribonuclease SacI; D015252:Deoxyribonucleases, Type II Site-Specific