[Characteristics of RecA-independent recombination of plasmids in E. coli cells producing restriction endonuclease EcoRI].
The restriction endonuclease EcoRI dependent recombination of compatible plasmids has been studied in RecA cells of Escherichia coli. Plasmid RP4 and the isogenic ColE1 type plasmids pSA14 or pSA25, differing in restriction-modification RM EcoRI genes, have been used to study this type of recombination. EcoRI dependent recombination of plasmids is demonstrated in RecA cells and, thus, is independent of general system of homologous recombination. The classes of recombinant plasmids isolated from RecA cells differ from the classes isolated from wild type cells. Levels of tetracycline resistance conferred by plasmid RP4 are shown to be dependent on the alleles of RecA+ gene, being extremely low in RecA cells. This property is demonstrated to be useful for obtaining the multicopy recombinant plasmids resulting from EcoRI dependent recombination in RecA cells of Escherichia coli.
D004262:DNA Restriction Enzymes / Q000096:biosynthesis*; D015246:Deoxyribonuclease EcoRI; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005819:Genetic Markers; D010957:Plasmids; D011995:Recombination, Genetic; D014161:Transduction, Genetic
D005819:Genetic Markers; D004262:DNA Restriction Enzymes; D015246:Deoxyribonuclease EcoRI
Osobennosti RecA-nezavisimoĭ rekombinatsii plazmid v kletkakh E. coli, produtsiruiushchikh éndonukleazu restriktsii EcoRI.