[Physical mapping of the immunity region of phage phi 80].
Mapping of the sites of cleavage of five restriction endonucleases (BglI, BglII, EcoRV, KpnI and PvuII) in the immunity region of bacteriophage phi 80 DNA is described. The order of the 27 restriction sites was established. This helped to localize the phi 80 cI gene within the 640 bp fragment of the immunity region. Recombinant plasmids carrying phi 80 "kil" function were constructed. The function is suppressed by the cI-coded repressor. The deletion AB43 which is present in the phi 80 vir mutant is located within the phi 80 DNA fragment carrying the cI gene.
D010582:Bacteriophage lambda / Q000235:genetics; D002874:Chromosome Mapping; D003090:Coliphages / Q000235:genetics*; D004262:DNA Restriction Enzymes; D005814:Genes, Viral; D005819:Genetic Markers; D005822:Genetic Vectors; D008242:Lysogeny; D010957:Plasmids
D005819:Genetic Markers; D004262:DNA Restriction Enzymes
Fizicheskoe kartirovanie oblasti immuniteta faga phi 80.