Purification and partial characterization of a calmodulin-stimulated nucleoside triphosphatase from pea nuclei.
A nucleoside triphosphatase/deoxynucleoside triphosphatase associated with the chromatin fraction from a highly purified preparation of pea nuclei has been isolated and characterized. The purified enzyme has a molecular weight of 47,000 as checked by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and it has an isoelectric point of 6.6. In the presence of divalent cations (Mg2+ = Mn2+ greater than Ca2+), this enzyme hydrolyzes nucleoside triphosphates or deoxynucleoside triphosphates. Hydrolysis is optimal at pH 7.5 and is significantly inhibited by relatively low concentrations of quercetin, but is not sensitive to vanadate, nitrate, or oligomycin. The enzyme has a rather broad nucleotide substrate specificity and has a Km for MgATP2- of 0.6 mM. The enzyme activity is stimulated over 3-fold by Ca2+ and calmodulin, and the stimulation is blocked by the Ca2+ chelator EGTA and by the calmodulin antagonists compound 48/80 and chlorpromazine.
D000255:Adenosine Triphosphate / Q000378:metabolism; D002118:Calcium / Q000494:pharmacology; D002147:Calmodulin / Q000494:pharmacology*; D002413:Cations, Divalent; D002467:Cell Nucleus / Q000201:enzymology; D004591:Electrophoresis, Polyacrylamide Gel; D007887:Fabaceae; D006863:Hydrogen-Ion Concentration; D007526:Isoelectric Point; D007700:Kinetics; D008970:Molecular Weight; D043583:Nucleoside-Triphosphatase; D010744:Phosphoric Monoester Hydrolases / Q000037:antagonists & inhibitors / Q000302:isolation & purification* / Q000378:metabolism; D010944:Plants / Q000201:enzymology*; D010946:Plants, Medicinal; D011794:Quercetin / Q000494:pharmacology; D013379:Substrate Specificity
D002147:Calmodulin; D002413:Cations, Divalent; D000255:Adenosine Triphosphate; D011794:Quercetin; D010744:Phosphoric Monoester Hydrolases; D043583:Nucleoside-Triphosphatase; D002118:Calcium