title: Effects of ethanol on gastric epithelial cell phospholipid dynamics and cellular function.
abstract: The lipid profile of isolated gastric superficial epithelial cells (SEC) was evaluated by proton nuclear magnetic resonance spectroscopy (1H-NMR). The most conspicuous resonance band in SEC spectra was due to the protons of +N(CH3)3 groups of phosphatidylcholine and, to a lesser degree, other phospholipid derivatives, on the basis of their chemical shift and addition of purified phospholipids. NMR of cell lysates and phospholipid extracts of SEC in deutero-chloroform provided further spectral resolution of these components. Phospholipase or ethanol treatments of SEC produced membrane disorganization reflected as increased peak intensity of the phospholipid signals. In addition, ethanol, in a dose-dependent manner, attenuated paranitrophenyl phosphatase activity, which correlated with inhibition of total and ouabain-sensitive 86Rubidium chloride uptake by SEC. This study suggests that NMR used in conjunction with other biochemical techniques can monitor SEC membrane structure-function relationships. NMR is a potentially powerful noninvasive probe to show changes in lipid membrane organization induced by low concentrations of ethanol (1%) and may indicate an early sign of "cytotoxicity" in intact SEC.
mesh_terms: D009597:4-Nitrophenylphosphatase / Q000378:metabolism; D000818:Animals; D002712:Chlorides / Q000378:metabolism; D004848:Epithelium / Q000378:metabolism; D000431:Ethanol / Q000494:pharmacology*; D005753:Gastric Mucosa / Q000187:drug effects / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008563:Membrane Lipids / Q000378:metabolism; D010713:Phosphatidylcholines / Q000378:metabolism; D010741:Phospholipases A / Q000494:pharmacology; D010743:Phospholipids / Q000378:metabolism*; D011817:Rabbits; D012413:Rubidium / Q000378:metabolism; D010738:Type C Phospholipases / Q000494:pharmacology
chemical_list: D002712:Chlorides; D008563:Membrane Lipids; D010713:Phosphatidylcholines; D010743:Phospholipids; D000431:Ethanol; D010741:Phospholipases A; D009597:4-Nitrophenylphosphatase; D010738:Type C Phospholipases; D012413:Rubidium; C032710:rubidium chloride