The distribution of GABA and glycine response in the mouse brain using Xenopus oocytes.
Injection of mRNA from different regions of the central nervous system (CNS) of the mouse into the Xenopus oocyte caused a difference in the relative glycine to gamma-aminobutyric acid (GABA) response of the oocyte. Glycine caused the response in oocytes injected with brainstem mRNA but hardly in oocytes injected with cerebrum mRNA and in oocytes injected with cerebellum mRNA. In contrast, GABA caused the response in oocytes injected with mRNA from each of the 3 parts of the CNS. These obvious regional differences may reflect the distribution of the receptors to each plausible neurotransmitter in the CNS.
D000818:Animals; D001923:Brain Chemistry; D001933:Brain Stem / Q000032:analysis; D002531:Cerebellum / Q000032:analysis; D005260:Female; D051379:Mice; D009865:Oocytes / Q000378:metabolism*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000302:isolation & purification; D011963:Receptors, GABA-A / Q000032:analysis*; D018009:Receptors, Glycine; D017981:Receptors, Neurotransmitter / Q000032:analysis*; D013116:Spinal Cord / Q000032:analysis; D014981:Xenopus
D012333:RNA, Messenger; D011963:Receptors, GABA-A; D018009:Receptors, Glycine; D017981:Receptors, Neurotransmitter