title: Strontium phosphate transfection of human cells in primary culture: stable expression of the simian virus 40 large-T-antigen gene in primary human bronchial epithelial cells.
abstract: Strontium ion formed DNA-phosphate precipitates analogous to those formed by calcium but lacking the lethal and differentiation-inducing effects of calcium on many epithelial cell types in primary culture. Human primary bronchial epithelial cells were transiently and stably transfected by using strontium phosphate; the frequency of stable transformation with a plasmid carrying the simian virus 40 large-T-antigen gene was greater than 10(-4).
mesh_terms: D000957:Antigens, Viral, Tumor / Q000235:genetics*; D001980:Bronchi / Q000166:cytology / Q000502:physiology*; D002478:Cells, Cultured; D011232:Chemical Precipitation; D004847:Epithelial Cells; D005786:Gene Expression Regulation; D005814:Genes, Viral; D006801:Humans; D010710:Phosphates; D013539:Simian virus 40; D013324:Strontium; D014162:Transfection
chemical_list: D000957:Antigens, Viral, Tumor; D010710:Phosphates; C052774:strontium phosphate; D013324:Strontium