title: [Primary structure of the potato virus X genome: the region preceding the capsid protein cistron].
abstract: DNA copies of the potato virus X (PVX) RNA corresponding to 2300 nucleotides at the 3'-end have been cloned. The cloned cDNA copies containing the nucleotides 445-1280 from the 3'-end have been sequenced. The 5'-terminal region of the PVX coat protein gene corresponds to residues 445-786 from the 3'-end. The amino acid sequences of two more open reading frames (ORF) have been deduced from the nucleotide sequence. The potential translation products of these ORF's would correspond to the nonstructural viral proteins. We have located the ORF1 within the region of residues 799-1009 preceding the coat protein cistron. The tentative protein is composed of 70 amino acids and has an aminoterminal segment which is markedly hydrophobic. ORF2 in the PVX sequence ends with UAG at nucleotides 942-944 and extends to the 5'-terminus for additional 340 nucleotides. The distant sequence homology exists between a carboxyterminal portion of PVX ORF2 and that of the nonstructural "30 K-proteins" of the plant tobamoviruses.
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000235:genetics; D004262:DNA Restriction Enzymes; D005796:Genes; D005814:Genes, Viral; D010942:Plant Viruses / Q000235:genetics*; D012315:RNA Caps / Q000032:analysis / Q000235:genetics; D011198:Solanum tuberosum / Q000382:microbiology*; D014759:Viral Envelope Proteins / Q000032:analysis*
chemical_list: D012315:RNA Caps; D014759:Viral Envelope Proteins; D004247:DNA; D004262:DNA Restriction Enzymes
vernacular_title: Izuchenie pervichnoĭ struktury genoma X-virusa kartofelia: oblast', predshestvuiushchaia tsistronu kapsidnogo belka.