title: Parental legacy determines methylation and expression of an autosomal transgene: a molecular mechanism for parental imprinting.
abstract: We have created a transgenic mouse strain in which an autosomal transgene bearing elements of the RSV LTR and a translocated c-myc gene obeys very unusual rules. If the transgene is inherited from the male parent, it is expressed in the heart and no other tissue. If it is inherited from the female parent, it is not expressed at all. This pattern of expression correlates precisely with a parentally imprinted methylation state evident in all tissues. Methylation of the transgene is acquired by its passage through the female parent and eliminated during gametogenesis in the male. These observations provide direct molecular evidence that autosomal gene expression can depend upon the sex of the parent from which the gene is inherited. They also provide a plausible mechanism for understanding parental imprinting that may be relevant to the failure of parthenogenesis in mammals, the apparent non-Mendelian behavior of some autosomal genes, and the role of methylation in gene regulation.
mesh_terms: D000818:Animals; D001358:Avian Sarcoma Viruses / Q000235:genetics*; D002460:Cell Line; D004274:DNA, Recombinant / Q000378:metabolism*; D004279:DNA, Viral / Q000235:genetics*; D005260:Female; D005786:Gene Expression Regulation; D007136:Immunoglobulins / Q000235:genetics; D008297:Male; D008745:Methylation; D051379:Mice; D009206:Myocardium / Q000378:metabolism; D009857:Oncogenes; D010053:Ovary / Q000378:metabolism; D010954:Plasmacytoma / Q000032:analysis; D012260:Ribonucleases / Q000378:metabolism; D013737:Testis / Q000378:metabolism; D014170:Transformation, Genetic; D014178:Translocation, Genetic
chemical_list: D004274:DNA, Recombinant; D004279:DNA, Viral; D007136:Immunoglobulins; D012260:Ribonucleases