Overproduction of single-stranded DNA-binding protein increases UV-induced mutagenesis in Escherichia coli.
UV-induced mutagenesis was investigated in the uvrB strain and its isogenic counterpart overproducing the single-stranded DNA-binding protein (SSB). It was demonstrated that overproduction of SSB significantly increases the frequency of mutation. Our results indicate that such an increase might be due to certain abnormalities in induction of the SOS response (untimely and prolonged activation of the RecA protein).
D004269:DNA, Bacterial / Q000235:genetics; D004277:DNA, Single-Stranded / Q000502:physiology*; D004268:DNA-Binding Proteins / Q000502:physiology*; D004307:Dose-Response Relationship, Radiation; D004926:Escherichia coli / Q000235:genetics; D005786:Gene Expression Regulation; D007700:Kinetics; D009154:Mutation / Q000528:radiation effects*; D011938:Rec A Recombinases / Q000502:physiology
D004269:DNA, Bacterial; D004277:DNA, Single-Stranded; D004268:DNA-Binding Proteins; D011938:Rec A Recombinases