[Detection and preliminary characteristics of the transforming gene (oncogene) of Ha-ras type in human stomach cancer].
High molecular weight DNA prepared from three undifferentiated human stomach carcinomas was assayed for transforming activity by transfection of mouse NIH 3T3 cells. One tumor DNA sample (stomach carcinoma CaVSt) induced (the transfection efficiency: 0.02 transformants/micrograms DNA X 10(-6) cells) transformation of NIH 3T3 recipient cells. Transforming gene of Ha-ras type was identified in transformants derived from this human carcinoma. The genetic lesion responsible for the activation of the CaVSt Ha-ras oncogene is not localized in the 12-th codon for p21c-Ha-ras protein.
D000230:Adenocarcinoma / Q000235:genetics; D000818:Animals; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D003062:Codon; D004262:DNA Restriction Enzymes; D004273:DNA, Neoplasm / Q000032:analysis*; D006801:Humans; D051379:Mice; D009154:Mutation; D009857:Oncogenes; D012091:Repetitive Sequences, Nucleic Acid; D013274:Stomach Neoplasms / Q000235:genetics*; D014162:Transfection
D003062:Codon; D004273:DNA, Neoplasm; D004262:DNA Restriction Enzymes
Obnaruzhenie i predvaritel'naia kharakteristika transformiruiushchego gena (onkogena) tipa Ha-ras raka zheludka cheloveka.