Purification and properties of DNA gyrase from a fluoroquinolone-resistant strain of Escherichia coli.
Subunit A and B proteins of DNA gyrase were separately purified from fluoroquinolone-resistant Escherichia coli GN14181 (MIC of ofloxacin, 100 micrograms/ml) and susceptible strain KL-16. The supercoiling activities of reconstituted Ar+Br (r, resistant) and Ar+Bs (s, susceptible) were 250-fold more resistant to new fluoroquinolones than those of As+Bs and As+Br.
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D004250:DNA Topoisomerases, Type II / Q000302:isolation & purification*; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology*; D006845:Hydrocarbons, Fluorinated / Q000494:pharmacology*
D000900:Anti-Bacterial Agents; D006845:Hydrocarbons, Fluorinated; D004250:DNA Topoisomerases, Type II