[Expression of the pectate lyase gene from Erwinia chrysanthemi ENA49 in cells of other Erwinia species].
The gene for a pectate lyase of E. chrysanthemi ENA49 cloned in a recombinant plasmid pPTL1 (a derivative of RSF1010) was transferred into E. carotovora. The pectate lyase determined by the cloned gene was secreted into the cultural medium from the cells of E. crysanthemi EC16. Partial secretion of the enzyme was registered for E. carotovora cells. The major part of EC1 E. chrysanthemi pectate lyase synthesized by E. carotovora cells is accumulated in periplasmic and cytoplasmic fractions. The obtained results suggest the different specificity or efficiency of pectate lyase secretion systems in the studied Erwinia strains.
D002874:Chromosome Mapping; D004262:DNA Restriction Enzymes; D004885:Erwinia / Q000201:enzymology* / Q000235:genetics; D005786:Gene Expression Regulation; D005798:Genes, Bacterial; D010957:Plasmids; D011133:Polysaccharide-Lyases / Q000235:genetics*; D013045:Species Specificity
D004262:DNA Restriction Enzymes; D011133:Polysaccharide-Lyases; C023911:pectate lyase
Ekspressiia gena pektatliazy Erwinia chrysanthemi ENA49 v kletkakh drugikh predstaviteleĭ roda Erwinia.