Human peripheral blood accessory cell: isolation by hypotonic density gradient, functional, and phenotypical characterization.
In order to purify the human peripheral blood-derived accessory cell that cooperate with T lymphocytes in the process of mitogenic stimulation, we developed a new density gradient separation. This was based on the principle of hypotonic swelling of the cells to obtain a differential change of the buoyant densities of cells. By this method, we have obtained a highly accessory cell-depleted lymphocyte fraction whose proliferative response to sodium periodate stimulation was almost aborted. Another fraction containing high accessory cell activity was further divided into Fc-receptor-positive and -negative cells. The latter revealed the highest accessory activity for T lymphocyte periodate stimulation. The cells were characterized according to a number of markers and appeared to resemble lymphoid dendritic cells. Compared with the monocyte/macrophage fraction, they showed veils and dendritiform elongations and expressed reduced values of monocyte/macrophage specific markers. Compared with the high accessory activity of these cells, monocytes/macrophages expressed a low accessory activity.
D000938:Antigen-Presenting Cells / Q000166:cytology; D002469:Cell Separation / Q000379:methods*; D002499:Centrifugation, Density Gradient / Q000379:methods; D003713:Dendritic Cells / Q000032:analysis / Q000502:physiology; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects; D008211:Lymphocyte Cooperation; D008264:Macrophages / Q000502:physiology; D009000:Monocytes / Q000502:physiology; D010504:Periodic Acid / Q000494:pharmacology; D010641:Phenotype; D011961:Receptors, Fc / Q000276:immunology; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000276:immunology
D011961:Receptors, Fc; D010504:Periodic Acid; C009288:metaperiodate