title: Activation of B lymphocytes by Epstein-Barr virus/CR2 receptor interaction.
abstract: Epstein-Barr virus (EBV) infects and transforms human B lymphocytes. The virus receptor was shown to be identical to the complement receptor CR2. The consequences of EBV/CR2 receptor interaction on B lymphocyte activation were analyzed by infection of B cells with the transforming (B95-8) and the nontransforming (P3HR1) virus strains and with UV-inactivated B95-8 virus. Similar to mitogens and antibodies against surface IgM and CR2 receptor, transforming and nontransforming EBV induced the release of leukocyte migration inhibitory factor (LIF). LIF production occurred early after infection and was not affected by irradiation of the B95-8 virus with doses of UV-light which prevented the expression of viral functions. B lymphocytes infected with UV-inactivated virus responded to T cell-derived B cell growth factors. The results demonstrate that binding of EBV to the CR2 receptor activates the B cells. Progression of the infected cells in the activation pathway required helper cell-derived factors or signals provided by the intact viral genome. The nontransforming P3HR1 virus induced a low level of RNA synthesis and increase of cell size and major histocompatibility complex class I antigen expression. Only the transforming virus induced expression of EBV nuclear antigens and cellular DNA synthesis.
mesh_terms: D000906:Antibodies / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology*; D002472:Cell Transformation, Viral; D003176:Complement C3 / Q000276:immunology; D004279:DNA, Viral / Q000096:biosynthesis; D005814:Genes, Viral; D006133:Growth Substances / Q000494:pharmacology; D004854:Herpesvirus 4, Human / Q000528:radiation effects; D015847:Interleukin-4; D007961:Leukocyte Migration-Inhibitory Factors / Q000096:biosynthesis; D008213:Lymphocyte Activation / Q000187:drug effects; D008222:Lymphokines / Q000494:pharmacology; D008934:Mitogens / Q000494:pharmacology; D012367:RNA, Viral / Q000096:biosynthesis; D011951:Receptors, Complement / Q000276:immunology / Q000378:metabolism*; D017464:Receptors, Complement 3d; D011991:Receptors, Virus / Q000276:immunology / Q000378:metabolism*; D013997:Time Factors; D014412:Tumor Virus Infections / Q000276:immunology*; D014466:Ultraviolet Rays
chemical_list: D000906:Antibodies; D003176:Complement C3; D004279:DNA, Viral; D006133:Growth Substances; D007961:Leukocyte Migration-Inhibitory Factors; D008222:Lymphokines; D008934:Mitogens; D012367:RNA, Viral; D011951:Receptors, Complement; D017464:Receptors, Complement 3d; D011991:Receptors, Virus; D015847:Interleukin-4