Transcript characterisation, gene disruption and nucleotide sequence of the Saccharomyces cerevisiae WH12 gene.
WH12 is a gene which plays a prominent role in regulating growth and proliferation in Saccharomyces cerevisiae. It is expressed as a 2.0-kb mRNA transcript. Disruption of this transcript in a WH12+ cell results in the mutant phenotype being displayed. The nucleotide sequence of the cloned gene has been determined and found to include a 487-codon long open reading frame coding for a 55.3-kDa protein. The protein showed no extensive homologies to any previously identified protein. The 5' and 3' noncoding regions contained many of the features found in other yeast genes.
D001483:Base Sequence; D002455:Cell Division; D003001:Cloning, Molecular; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014158:Transcription, Genetic