Comparison of the Abbott and Ortho enzyme immunoassays and cell culture for the detection of respiratory syncytial virus in nasopharyngeal specimens.
A comparison of the Abbott Laboratories and the Ortho Diagnostic Systems Respiratory Syncytial Virus (RSV) Enzyme Immunoassays (EIA) and HEp-2 cell culture for the detection of RSV in 81 nasopharyngeal (NP) specimens from pediatric patients with lower respiratory tract infection was carried out. The sensitivity and specificity of the Abbott test compared to confirmed infection was 92.3% and 100.0%, respectively. The sensitivity and specificity of the Ortho test was 87.5% and 80.3%, respectively. We found the Abbott EIA test to be sensitive, specific, rapid, and easy to perform.
D000956:Antigens, Viral / Q000032:analysis; D006801:Humans; D007124:Immunoenzyme Techniques; D007223:Infant; D009305:Nasopharynx / Q000378:metabolism / Q000382:microbiology*; D011933:Reagent Kits, Diagnostic; D012136:Respiratory Syncytial Viruses / Q000276:immunology / Q000302:isolation & purification*; D012141:Respiratory Tract Infections / Q000175:diagnosis*; D010253:Respirovirus Infections / Q000175:diagnosis*; D007507:Therapeutic Irrigation
D000956:Antigens, Viral; D011933:Reagent Kits, Diagnostic