title: [Directed mutagenesis of chromosomal genes of Escherichia coli in vivo: construction of RecA- and HtpR- mutants].
abstract: The deletions in Escherichia coli chromosomal genes recA and htpR were constructed using the site-directed mutagenesis techniques. The obtained RecA- mutants are UV-sensitive and have a phenotype defective for the homologous DNA recombination. HtpR- mutant is temperature sensitive for growth and deficient in intracellular proteolysis. As a result a HtpR- mutant seems to be a preferable candidate for attempting to synthesize efficiently any alien protein in Escherichia coli cells.
mesh_terms: D002876:Chromosomes, Bacterial; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D009154:Mutation; D010957:Plasmids; D014169:Transformation, Bacterial
chemical_list: D004274:DNA, Recombinant; D004262:DNA Restriction Enzymes
vernacular_title: Napravlennyĭ mutagenez khromosomnykh genov Escherichia coli in vivo: konstruirovanie RecA- i HtpR- mutantov.