title: Similarities in cAMP responses between murine lymphoid cell lines and subsets of mouse lymphocytes.
abstract: Intracellular cAMP responses to forskolin (which stimulates independently of hormone receptors) and to several hormones were studied in mouse lymphoid cell lines of B- and T-cell origin and in subpopulations of mouse lymphocytes. In the T-cell lines, isoproterenol caused an increase in cAMP amounting at most to 28% of that caused by 100 microM forskolin. In contrast, in the B-cell lines the increase ranged from 50% to over 100%. In 11 of 13 T-cell lines, forskolin at 1 microM concentration potentiated the isoproterenol response five- to tenfold. Forskolin potentiation was also found in thymocytes and peripheral T-cells but not in B-cell lines. The relative increases in intracellular cAMP evoked by isoproterenol, prostaglandin E2 (PGE2) and histamine varied markedly from cell line to cell line. For instance, three lines carrying the T-helper cell marker L3T4 responded only to PGE2 and not to isoproterenol, whereas two cell lines carrying the Ly2 marker gave a higher response to isoproterenol than to PGE2. The results indicate that subsets of lymphocytes respond differently to various hormones and that these differences are maintained in continuous cell lines.
mesh_terms: D000818:Animals; D002460:Cell Line; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000032:analysis*; D015232:Dinoprostone; D004357:Drug Synergism; D004789:Enzyme Activation; D006632:Histamine / Q000494:pharmacology; D007545:Isoproterenol / Q000494:pharmacology; D008214:Lymphocytes / Q000032:analysis / Q000145:classification / Q000187:drug effects*; D051379:Mice; D011458:Prostaglandins E / Q000494:pharmacology; D011494:Protein Kinases / Q000032:analysis
chemical_list: D011458:Prostaglandins E; D005576:Colforsin; D006632:Histamine; D000242:Cyclic AMP; D011494:Protein Kinases; D015232:Dinoprostone; D007545:Isoproterenol