Sensitivity and specificity of enzyme immunofiltration and DNA hybridization for the detection of HCMV-infected cells.
The sensitivity and specificity of enzyme immunofiltration and DNA hybridization were compared in human cytomegalovirus (HCMV) (AD 169)-infected MRC-5 cells. The enzyme immunofiltration was carried out on glass fiber filters in microplates, using an HCMV (AD 169) monoclonal antibody and a peroxidase conjugate. The DNA hybridization was carried out with a microfiltration apparatus, using a 32P-labelled HCMV (AD 169) Eco R1 D fragment probe. The sensitivities of enzyme immunofiltration and DNA hybridization were 1.82 X 10(3) and 1.13 X 10(3) infected cells, respectively. Both methods were highly specific, but enzyme immunofiltration was faster and simpler.
D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology; D000956:Antigens, Viral / Q000032:analysis*; D001345:Autoradiography; D002460:Cell Line; D003587:Cytomegalovirus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D004279:DNA, Viral / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007124:Immunoenzyme Techniques; D007163:Immunosorbent Techniques; D009693:Nucleic Acid Hybridization; D011237:Predictive Value of Tests; D012044:Regression Analysis
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D004279:DNA, Viral