title: IS2 activates the ilvA gene of Pseudomonas cepacia in Escherichia coli.
abstract: The ilvA gene of Pseudomonas cepacia was expressed poorly in Escherichia coli. Insertion of IS2 upstream of the cloned gene dramatically increased its transcription, resulting in an 85-fold increase in threonine dehydratase (deaminase) specific activity. The results confirm earlier reports that IS2 promotes efficient expression of foreign genes in E. coli.
mesh_terms: D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005786:Gene Expression Regulation; D005798:Genes, Bacterial; D011549:Pseudomonas / Q000201:enzymology / Q000235:genetics*; D013913:Threonine Dehydratase / Q000096:biosynthesis / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D004251:DNA Transposable Elements; D013913:Threonine Dehydratase