Signal recognition particle arrests elongation of nascent secretory and membrane proteins at multiple sites in a transient manner.
The signal recognition particle (SRP) has been shown to target nascent secretory and membrane proteins to the endoplasmic reticulum. In the wheat germ cell-free system, SRP arrests the elongation of the nascent chains until the translational complex is docked to the endoplasmic reticulum membrane where the interaction between SRP and docking protein causes a release of the nascent chain arrest. For two secretory proteins, arrested peptides of 70 amino acids have been identified (Walter, P., Ibrahimi, I., and Blobel, G. (1981) J. Cell Biol. 91, 545-550; Meyer, D. I., Krause, E., and Dobberstein, B. (1982) Nature 297, 647-650). By using an in vitro coupled transcription-translation system, we have analyzed SRP arrest and the resulting peptides of the two secretory proteins lysozyme and granulocyte-macrophage colony-stimulating factor and the membrane protein invariant chain. SRP arrested the elongation of all three proteins at multiple sites, giving rise to ladders of arrested peptides. The size of the arrested peptides increased with the time of translation, resulting in mostly full-length pre-peptides after about 40 min. This suggests that SRP arrest in transient rather than stable. Upon addition of microsomes, the SRP arrest was released, and all the blocked peptides could be chased into mature proteins or full-length precursors.
D000818:Animals; D002645:Chickens; D006680:HLA Antigens / Q000096:biosynthesis; D007147:Immunoglobulin Light Chains / Q000096:biosynthesis; D007163:Immunosorbent Techniques; D008565:Membrane Proteins / Q000096:biosynthesis*; D051379:Mice; D009113:Muramidase / Q000096:biosynthesis; D010441:Peptide Chain Elongation, Translational / Q000187:drug effects*; D014176:Protein Biosynthesis; D012261:Ribonucleoproteins / Q000494:pharmacology*; D018271:Signal Recognition Particle
D006680:HLA Antigens; D007147:Immunoglobulin Light Chains; D008565:Membrane Proteins; D012261:Ribonucleoproteins; D018271:Signal Recognition Particle; C503637:Srp72 protein, mouse; D009113:Muramidase