Isolation of a biotin receptor from hepatic plasma membranes.
Biotin, one of the growth promoting members of the B complex vitamin family, was found in the present investigation to have a specific receptor on isolated plasma membranes. Biotin bound to its receptor in a linear manner at 20 degrees C with some binding as early as 30 minutes and full equilibrium binding being present at 20 hours. The best binding was found at 0.6 mg/ml of protein, but significant binding was still present at 0.6 microgram/ml. The saturation of ligand binding sites was at 10(-7)M. Half maximal saturation of binding was between 10(-9) and 10(-10)M. These results demonstrate that a receptor for biotin does exist on purified plasma membranes.
D000818:Animals; D001710:Biotin / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D066298:In Vitro Techniques; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D051379:Mice; D011956:Receptors, Cell Surface / Q000302:isolation & purification* / Q000378:metabolism; D017978:Receptors, Growth Factor
D011956:Receptors, Cell Surface; D017978:Receptors, Growth Factor; C052136:biotin receptor; D001710:Biotin