title: Analysis of bacterial exopolysaccharides.
abstract: Extracellular polysaccharides have been isolated from cultures of freshwater and marine bacteria originally isolated from material adhering to surfaces and underivatized hydrolysates have been analyzed by high-performance liquid chromatography methods. A scheme has been developed whereby the uronic acids can be identified on strong anion-exchange columns, while neutral monosaccharides can be separated and identified using aminobonded columns or cation-exchange adsorbent loaded with a heavy metal ion. The methods permit rapid and accurate comparison of polysaccharides with differing chemotype. The strains studied show a range of different chemotypes, all containing a uronic acid and several neutral monosaccharides. Some of the polysaccharides isolated from marine bacteria possessed a very high acetyl content.
mesh_terms: D002851:Chromatography, High Pressure Liquid; D002854:Chromatography, Paper; D003124:Colorimetry; D006868:Hydrolysis; D010084:Oxidation-Reduction; D010504:Periodic Acid; D011135:Polysaccharides, Bacterial / Q000032:analysis* / Q000302:isolation & purification
chemical_list: D011135:Polysaccharides, Bacterial; D010504:Periodic Acid