title: Endosomal compartment of toad bladder epithelium.
abstract: Apical exocytosis and increased permeability are induced by antidiuretic hormone (ADH). After this, endocytosis is also induced by ADH and is associated with the decline in ADH-induced water permeability at the apical surface of the toad urinary bladder (9, 19, 20). During this process, horseradish peroxidase (HRP), a fluid phase marker, is taken up from the mucosal solution into endocytic tubules and multivesicular bodies. We now report that we can introduce from the apical (mucosal) side, a viral transmembrane protein (the G-protein of VSV) and that this protein can be retrieved as an integral membrane protein in endocytic membranes. This was demonstrated by immunoisolation of endosomal vesicles loaded with HRP using a monoclonal antibody against the cytoplasmic domain of the G-protein.
mesh_terms: D000818:Animals; D002025:Bufonidae; D003594:Cytoplasmic Granules / Q000502:physiology / Q000648:ultrastructure*; D004705:Endocytosis; D004848:Epithelium / Q000502:physiology / Q000648:ultrastructure; D005260:Female; D008562:Membrane Glycoproteins; D008854:Microscopy, Electron; D008871:Microvilli / Q000502:physiology / Q000648:ultrastructure; D001743:Urinary Bladder / Q000502:physiology / Q000648:ultrastructure*; D014721:Vesicular stomatitis Indiana virus; D014759:Viral Envelope Proteins; D014764:Viral Proteins / Q000378:metabolism
chemical_list: C028391:G protein, vesicular stomatitis virus; D008562:Membrane Glycoproteins; D014759:Viral Envelope Proteins; D014764:Viral Proteins