A high resolution diffracting crystal form of the complex between yeast tRNAAsp and aspartyl-tRNA synthetase.
Three new crystal forms of the complex between yeast tRNAAsp and aspartyl-tRNA synthetase have been produced. The best crystals, obtained after modifying both purification and crystallization conditions, belong to space group P2(1)2(1)2(1) and diffract to 2.7 A. Unit cell parameters are a = 210.4 A, b = 145.3 A and c = 86.0 A (1 A = 0.1 nm), with one dimeric enzyme and two tRNA molecules in the asymmetric unit.
D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism*; D001226:Aspartate-tRNA Ligase / Q000378:metabolism*; D003460:Crystallization; D012331:RNA, Fungal / Q000378:metabolism*; D012345:RNA, Transfer, Amino Acid-Specific / Q000378:metabolism*; D012349:RNA, Transfer, Asp / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D014961:X-Ray Diffraction
D012331:RNA, Fungal; D012345:RNA, Transfer, Amino Acid-Specific; D012349:RNA, Transfer, Asp; D000604:Amino Acyl-tRNA Synthetases; D001226:Aspartate-tRNA Ligase