The propagation of feline panleukopenia virus in micro-carrier cell culture and use of the inactivated virus in the protection of mink against viral enteritis.
Using microcarrier cell culture for the production of virus antigen, a formalin-inactivated feline panleukopenia virus vaccine was evaluated for protection of mink against specific mink enteritis virus infection. The vaccine showed a good immunogenic effect in mink when used either alone or in combination with Clostridium botulinum type C-toxoid and/or Pseudomonas aeruginosa vaccine. A single vaccination induced persistent immune responses for periods of at least 1 year, as evaluated by ELISA and challenge tests. Neither immunological interference between vaccine constituents nor adverse reactions were observed.
D000818:Animals; D000956:Antigens, Viral / Q000276:immunology*; D002478:Cells, Cultured; D005254:Feline Panleukopenia / Q000517:prevention & control*; D028301:Feline Panleukopenia Virus / Q000254:growth & development* / Q000276:immunology; D008907:Mink / Q000276:immunology*; D010321:Parvoviridae / Q000254:growth & development*; D014765:Viral Vaccines / Q000276:immunology*
D000956:Antigens, Viral; D014765:Viral Vaccines