title: Cloned human interferons alpha: differential affinities for polyinosinic acid and relationship between molecular structure and species specificity.
abstract: The HuIFN-alpha A and HuIFN-alpha D interferons, produced by two independent recombinant bacterial clones, have different affinities for polyinosinic acid (poly I). The monomeric form HuIFN-alpha A (FMM), but not the HuIFN-alpha D, binds to poly (I)-agarose and is protected by poly (I) from thermal inactivation. Other subtypes of HuIFN-alpha A including the monomer SMM and oligomers have no affinity for this polynucleotide. In addition, these interferons show different target cell preferences in agreement with our previous suggestion (23) that the polynucleotide binding domain may be responsible for species specificity. Two significant observations are 1) the fractions of HuIFN-alpha D and HuIFN-alpha A unbound on poly (I)-agarose show higher antiviral inducing activity on heterologous (MDBK) than on homologous (WISH) cells, whereas they induce about the same activity of 2'5' oligoadenylate synthetase in these two cell lines. These fractions are also active on L929 cells. 2) The bound fraction of HuIFN-alpha A induces almost the same antiviral and 2'5' oligoadenylate synthetase activities in MDBK and in WISH cells but neither activity in L929 cells.
mesh_terms: D015088:2',5'-Oligoadenylate Synthetase / Q000096:biosynthesis; D000818:Animals; D002460:Cell Line; D004355:Drug Stability; D004790:Enzyme Induction; D006801:Humans; D007370:Interferon Type I / Q000378:metabolism* / Q000494:pharmacology; D011069:Poly I / Q000378:metabolism* / Q000494:pharmacology; D011131:Polyribonucleotides / Q000378:metabolism*; D011485:Protein Binding; D011994:Recombinant Proteins / Q000378:metabolism* / Q000494:pharmacology; D013045:Species Specificity; D013329:Structure-Activity Relationship; D014721:Vesicular stomatitis Indiana virus / Q000187:drug effects
chemical_list: D007370:Interferon Type I; D011131:Polyribonucleotides; D011994:Recombinant Proteins; D011069:Poly I; D015088:2',5'-Oligoadenylate Synthetase