Phorbol ester stimulates human granulosa-luteal cell cyclic adenosine 3', 5'-monophosphate and progesterone production.
Human granulosa-luteal cell production of cyclic adenosine 3', 5'-monophosphate (cAMP) and progesterone (P) were studied in response to 12-O-tetradecanoyl-phorbol-13-acetate (TPA). TPA specifically increased cAMP synthesis in a dose-dependent manner. A 7-fold increase occurred at a TPA concentration of 1 ng/ml. Time-course studies indicated that the increase in accumulation of cAMP into culture media became detectable at 4 h and continued up to 72 h. TPA also enhanced P synthesis, but the increase was statistically significant only at 72 h. Indomethacin prevented TPA-stimulated cAMP and P production. The results suggest that TPA stimulates granulosa-luteal cell cAMP and P production, and that the action of TPA is mediated by the increase in prostaglandin synthesis.
D003338:Corpus Luteum / Q000187:drug effects* / Q000378:metabolism; D000242:Cyclic AMP / Q000096:biosynthesis*; D004305:Dose-Response Relationship, Drug; D005260:Female; D006107:Granulosa Cells / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology; D010703:Phorbol Esters / Q000494:pharmacology*; D011374:Progesterone / Q000096:biosynthesis*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013997:Time Factors
D010703:Phorbol Esters; D011374:Progesterone; D000242:Cyclic AMP; D013755:Tetradecanoylphorbol Acetate; D007213:Indomethacin