Megabase-scale mapping of the HLA gene complex by pulsed field gel electrophoresis.
In the study of the genetic structure of mammalian chromosomes, there exists a "resolution gap" between molecular cloning experiments and meiotic linkage analyses. This gap has discouraged attempts to construct full-scale genetic maps of mammalian chromosomes. The organization of the human major histocompatibility complex was examined within this range by pulsed field gel electrophoresis. The data obtained indicate that the complex spans over 3000 kilobases and enable the construction of a megabase-scale molecular map. These results indicate that the techniques employed in DNA extraction, enzymatic digestion, electrophoresis, and hybridization are suitable for the efficient analysis of megabase regions of mammalian chromosomes and effectively bridge the resolution gap between molecular cloning and classical genetics.
D000818:Animals; D001482:Base Composition; D004247:DNA / Q000235:genetics; D004262:DNA Restriction Enzymes; D004586:Electrophoresis; D006680:HLA Antigens / Q000235:genetics*; D006681:HLA-D Antigens / Q000235:genetics; D006801:Humans; D008285:Major Histocompatibility Complex; D051379:Mice; D009693:Nucleic Acid Hybridization
D006680:HLA Antigens; D006681:HLA-D Antigens; D004247:DNA; D004262:DNA Restriction Enzymes