title: Compared roles of glucose, galactose and fructose as glycogen precursors during the acute response to insulin in cultured rat foetal hepatocytes.
abstract: 1. The efficiency of the contribution of hexoses to basal- and stimulated-glycogenesis, when studied in cultured 18 day-old rat foetal hepatocytes in the presence of glucose, was as follows: galactose greater than glucose greater than fructose. 2. Glucose deprivation had opposite effects on the contributions of [14C]galactose (decreased) and [14C]fructose (increased) to glycogenesis, which occurred independently of insulin and were reversed by glucose concentrations as low as 30-100 microM. 3. The stimulation of glycogenesis by insulin measured with [14C]glucose (3.2-fold) was superior to that obtained with either [14C]galactose or [14C]fructose (2.7-fold in both cases), which revealed a specific beneficial effect of insulin on glucose contribution.
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D005632:Fructose / Q000502:physiology*; D005690:Galactose / Q000502:physiology*; D005947:Glucose / Q000502:physiology*; D007328:Insulin / Q000494:pharmacology*; D007773:Lactates / Q000096:biosynthesis; D008099:Liver / Q000187:drug effects / Q000196:embryology / Q000502:physiology*; D008112:Liver Glycogen / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D007328:Insulin; D007773:Lactates; D008112:Liver Glycogen; D005632:Fructose; D005947:Glucose; D005690:Galactose