Fractionation of Theiler's virus-infected BHK21 cell homogenates: isolation of virus-induced membranes.
A purified fraction containing unique membranes entrapping virions was isolated from homogenates of cells infected with the DA strain of Theiler's virus, after high-speed centrifugation through a sucrose gradient. This fraction, sedimented at 45-50% sucrose, was only found in cells infected with the DA strain but not in cells infected with the GDVII strain of Theiler's virus or in mock-infected cells. Immunogold staining of the membranes entrapping virions, using antivirus IgG antibodies, revealed that the membranes entrapping virions did not incorporate viral capsid antigens.
D000818:Animals; D000956:Antigens, Viral / Q000276:immunology; D002213:Capsid / Q000276:immunology; D002458:Cell Fractionation; D002460:Cell Line; D002462:Cell Membrane / Q000032:analysis* / Q000382:microbiology; D002499:Centrifugation, Density Gradient; D006224:Cricetinae; D004770:Enterovirus / Q000302:isolation & purification*; D005347:Fibroblasts / Q000382:microbiology / Q000648:ultrastructure*; D007668:Kidney; D004680:Maus Elberfeld virus / Q000276:immunology / Q000302:isolation & purification*; D008647:Mesocricetus; D014771:Virion / Q000302:isolation & purification*
D000956:Antigens, Viral