Human gastrin-releasing peptide gene is located on chromosome 18.
Gastrin-releasing peptide (GRP), a bombesin-like peptide, increases plasma levels of gastrin, pancreatic polypeptide, glucagon, gastric inhibitory peptide, and insulin. GRP is produced in large quantities by small-cell lung cancer and acts as a growth factor for these cells. To determine if chromosomal changes in small-cell lung cancer are related to the expression of GRP, we chromosomally mapped the gene using human-mouse somatic cell hybrids. Twenty hybrids, characterized for human chromosomes, were analyzed by Southern filter hybridization of DNA digested with EcoRI. Human DNA cut with EcoRI yields a major band of 6.8 kb and a minor band of 11.3 kb. The 6.8 kb band segregated concordantly with chromosome 18 and the marker peptidase A. The chromosome 3 abnormalities seen in small-cell lung cancer do not correlate with the chromosomal location of GRP, suggesting that the elevated expression of this gene may be due to mechanisms other than chromosomal rearrangement.
D000818:Animals; D002874:Chromosome Mapping; D002887:Chromosomes, Human, Pair 18; D004247:DNA / Q000235:genetics; D004262:DNA Restriction Enzymes; D019886:Gastrin-Releasing Peptide; D006801:Humans; D006822:Hybrid Cells; D051379:Mice; D010455:Peptides / Q000235:genetics*
D010455:Peptides; D019886:Gastrin-Releasing Peptide; D004247:DNA; D004262:DNA Restriction Enzymes