Mutagenicity studies on ketone solvents: methyl ethyl ketone, methyl isobutyl ketone, and isophorone.
3 ketone solvents (methyl ethyl ketone (MEK), methyl isobutyl ketone (MiBK), and isophorone) were tested for potential genotoxicity. The assays of MEK and MiBK included the Salmonella/microsome (Ames) assay, L5178Y/TK+/- mouse lymphoma (ML) assay, BALB/3T3 cell transformation (CT) assay, unscheduled DNA synthesis (UDS) assay, and micronucleus (MN) assay. Only the ML, UDS, and MN assays were conducted on samples of isophorone. No genotoxicity was found for MEK or isophorone. The presence of a marginal response only at the highest, cytotoxic concentration tested in the ML assay, the lack of reproducibility in the CT assay, and clearly negative results in the Ames assay, UDS and MN assays, suggest that MiBK is unlikely to be genotoxic in mammalian systems.
D000818:Animals; D002074:Butanones / Q000494:pharmacology*; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D003510:Cyclohexanes / Q000494:pharmacology*; D003512:Cyclohexanones / Q000494:pharmacology*; D004261:DNA Replication / Q000187:drug effects; D005260:Female; D007659:Ketones / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008223:Lymphoma / Q000473:pathology; D008297:Male; D008742:Methyl n-Butyl Ketone / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008862:Microsomes, Liver / Q000378:metabolism; D009152:Mutagenicity Tests / Q000379:methods; D009153:Mutagens / Q000494:pharmacology*; D012486:Salmonella typhimurium / Q000187:drug effects; D012997:Solvents
D002074:Butanones; D003510:Cyclohexanes; D003512:Cyclohexanones; D007659:Ketones; D009153:Mutagens; D012997:Solvents; C005940:isophorone; C005222:methylethyl ketone; D008742:Methyl n-Butyl Ketone; C005458:methyl isobutyl ketone