Multiple protein-binding sites in the 5'-flanking region regulate c-fos expression.
We tested sequences flanking the mouse c-fos gene for the ability to form specific DNA-protein complexes with factors present in crude nuclear extracts prepared from mammalian cells. Three such complexes were detected. One complex formed in a region necessary for the induction of c-fos expression by serum growth factors. Two additional complexes formed at sequences that contribute to basal c-fos promoter activity in vivo. These complexes represent three novel sequence-specific DNA-binding activities which appear to participate in the regulation of c-fos transcription.
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002478:Cells, Cultured; D002872:Chromosome Deletion; D004262:DNA Restriction Enzymes; D004268:DNA-Binding Proteins / Q000378:metabolism*; D005809:Genes, Regulator; D008223:Lymphoma; D008745:Methylation; D051379:Mice; D008807:Mice, Inbred BALB C; D009857:Oncogenes; D014158:Transcription, Genetic
D004268:DNA-Binding Proteins; D004262:DNA Restriction Enzymes