[Cloning of the gene of Saccharomyces cerevisiae yeasts that determines resistance to the toxic action of cadmium ions].
A gene conferring resistance to cadmium in Saccharomyces cerevisiae was isolated from a yeast gene library created on the basis of the pL3 vector. The phenotype of resistance is only expressed in the yeast cells with cloned DNA inserted into a multicopy plasmid. Integration of the plasmid into chromosome or introduction of the centromeric region into the plasmid decreases the level of cadmium resistance. The cloned Sau3A I fragment of the yeast chromosome is 3.5 kbp in size. Restriction analysis and subcloning experiments showed the gene to be located within 1.6 kbp of the XhoI-Sau3A I fragment of DNA. Instability was observed in the vicinity of the XhoI-Sau3A I fragment of the yeast DNA in Escherichia coli.
D001483:Base Sequence; D002104:Cadmium / Q000037:antagonists & inhibitors* / Q000633:toxicity; D019187:Cadmium Compounds; D003001:Cloning, Molecular / Q000187:drug effects / Q000379:methods*; D004271:DNA, Fungal / Q000187:drug effects / Q000235:genetics; D004352:Drug Resistance, Microbial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005800:Genes, Fungal / Q000187:drug effects*; D005838:Genotype; D010957:Plasmids / Q000187:drug effects; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics*; D013431:Sulfates
D019187:Cadmium Compounds; D004271:DNA, Fungal; D013431:Sulfates; D002104:Cadmium; C037123:cadmium sulfate
Klonirovanie gena drozhzheĭ Saccharomyces cerevisiae, opredeliaiushchego rezistentnost' k toksicheskomu deĭstviiu ionov kadmiia.