Expression of polyomavirus large T antigen by using a baculovirus vector.
A gene encoding the large T antigen of polyomavirus was inserted into the baculovirus Autographa californica nuclear polyhedrosis virus so that gene expression was under the control of the strong, very late polyhedrin gene promoter. Significantly more large T antigen was produced in recombinant virus-infected insect cells than was observed in polyomavirus-transformed mouse cells. The insect-derived T antigen exhibited polyomavirus origin-specific DNA binding. The baculovirus expression system provides a convenient source of T antigen for in vitro studies.
D000818:Animals; D000911:Antibodies, Monoclonal; D000957:Antigens, Viral, Tumor / Q000235:genetics*; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000235:genetics; D005822:Genetic Vectors; D007304:Insect Viruses / Q000235:genetics; D000077183:Occlusion Body Matrix Proteins; D011120:Polyomavirus / Q000235:genetics*; D011401:Promoter Regions, Genetic; D014764:Viral Proteins / Q000235:genetics; D015678:Viral Structural Proteins
D000911:Antibodies, Monoclonal; D000957:Antigens, Viral, Tumor; D004268:DNA-Binding Proteins; D000077183:Occlusion Body Matrix Proteins; D014764:Viral Proteins; D015678:Viral Structural Proteins; C009829:polyhedrin protein, Nucleopolyhedrovirus