The effect of human recombinant macrophage colony-stimulating factor (CSF-1) on the murine mononuclear phagocyte system in vivo.
Human recombinant macrophage CSF (CSF-1) was administered i.v. to mice. After four daily injections there was a dose-dependent increase in the responsiveness of bone marrow cells from the treated animals to CSF-1 in vitro. At the highest dose tested (20,000 U/day) there was a selective 10-fold increase in the circulating population of mature monocytes. CSF-1 treatment also increased the macrophage content of the liver and peritoneal cavity and caused splenomegaly. The macrophages isolated from the peritoneum of CSF-1-treated animals were larger and expressed higher levels of the macrophage-specific F4/80 Ag. These data demonstrate that CSF-1 can act as a circulating regulator of the mononuclear phagocyte system.
D000818:Animals; D001853:Bone Marrow / Q000187:drug effects / Q000378:metabolism; D003115:Colony-Stimulating Factors / Q000008:administration & dosage*; D004906:Erythrocyte Count / Q000187:drug effects; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D006133:Growth Substances / Q000008:administration & dosage*; D006801:Humans; D007958:Leukocyte Count / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000276:immunology; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051379:Mice; D010529:Peritoneal Cavity; D011994:Recombinant Proteins / Q000008:administration & dosage*; D013154:Spleen / Q000187:drug effects / Q000276:immunology; D013936:Thymidine / Q000378:metabolism
D003115:Colony-Stimulating Factors; D006133:Growth Substances; D011994:Recombinant Proteins; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D013936:Thymidine