HLA-B27 in inbred and non-inbred transgenic mice. Cell surface expression and recognition as an alloantigen in the absence of human beta 2-microglobulin.
A gene encoding the H chain of the human class I MHC Ag HLA-B27 was introduced into the germ lines of inbred C57BL/6 (B6) and non-inbred (B6 X SJL/J) F2 mice. By immunofluorescence and flow cytometry, the HLA-B27 gene product was expressed on lymphoid cells at levels comparable to the endogenous H-2b and H-2s class I MHC molecules. In both primary and secondary MLC between responder spleen cells from non-transgenic (B6 X SJL/J) F1 mice and transgenic stimulator cells, CTL were generated that specifically lysed mouse L cell (H-2k) or human B cell targets expressing HLA-B27, and this lysis thus appeared largely unrestricted by H-2. These results indicate that transgenic mice express a functional HLA-B27 gene product on cell surfaces in the absence of the human beta 2-microglobulin gene. These transgenic mice promise to be a valuable resource in the investigation of the unique role of HLA-B27 in inflammatory human disease.
D000818:Animals; D000954:Antigens, Surface / Q000235:genetics* / Q000276:immunology; D005854:Germ Cells / Q000378:metabolism; D015235:HLA-B Antigens / Q000032:analysis / Q000235:genetics*; D015796:HLA-B27 Antigen; D051379:Mice; D008810:Mice, Inbred C57BL / Q000235:genetics*; D008822:Mice, Transgenic / Q000235:genetics*; D010375:Pedigree; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014162:Transfection; D001613:beta 2-Microglobulin / Q000235:genetics*
D000954:Antigens, Surface; D015235:HLA-B Antigens; D015796:HLA-B27 Antigen; D001613:beta 2-Microglobulin