Cloning of myelomas and hybridomas in fibrin clots.
Myelomas and hybridomas were observed to proliferate normally when trapped in a fibrin clot. The fibrin clot was obtained by including fibrinogen in the cell culture medium and thrombin in the plastic dish. A clot formed within seconds and cloning efficiency was around 100%. This technique has all the advantages of semi-solid medium cloning but avoids toxicity to the cells and the exposure to high temperature associated with the soft-agar cloning technique.
D000818:Animals; D001777:Blood Coagulation; D002455:Cell Division; D002460:Cell Line; D002999:Clone Cells / Q000166:cytology*; D003470:Culture Media; D005337:Fibrin; D005340:Fibrinogen; D006825:Hybridomas / Q000166:cytology*; D007158:Immunologic Techniques; D007958:Leukocyte Count; D051379:Mice; D010954:Plasmacytoma / Q000276:immunology*; D013917:Thrombin
D003470:Culture Media; D005337:Fibrin; D005340:Fibrinogen; D013917:Thrombin