An in vivo complex with DNA photolyase blocks UV mutagenesis targeted at a thymine-cytosine dimer in Escherichia coli.
UV mutation frequency responses for two types of Escherichia coli prototrophic mutant were measured. Only the response associated with a mutation targeted by a thymine-cytosine pyrimidine dimer was reduced in the dark in cells with amplified DNA photolyase. This specific reduction is attributed to the interruption of mutational DNA synthesis by a photolyase complex at the targeting dimer.
D004255:Deoxyribodipyrimidine Photo-Lyase / Q000378:metabolism*; D004307:Dose-Response Relationship, Radiation; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics / Q000528:radiation effects*; D008190:Lyases / Q000378:metabolism*; D009154:Mutation; D011740:Pyrimidine Dimers; D014158:Transcription, Genetic; D014466:Ultraviolet Rays
D011740:Pyrimidine Dimers; D008190:Lyases; D004255:Deoxyribodipyrimidine Photo-Lyase