Vanadate inhibition of rat cerebral cortex Na+,K+-ATPase during postnatal development.
The Na+,K+-ATPase activity and its response to vanadate inhibition was investigated in cerebral cortex homogenates of 7-, 12- and 18-day-old rats. The enzyme was inhibited by vanadate in a dose-dependent manner in all these age groups. Furthermore, there was a different sensitivity towards vanadate during postnatal development; the concentration of V+5 needed for 50% inhibition of Na+, K+-ATPase was 1.1 X 10(-6)M, 2 X 10(-7)M and 4.4 X 10(-7)M for 7-, 12- and 18-day-old rats, respectively. It is suggested that the different sensitivity of Na+, K+-ATPase towards vanadate inhibition during postnatal development might be due to age-dependent changes in the ratio of various cell types.
D000375:Aging / Q000378:metabolism; D000818:Animals; D002540:Cerebral Cortex / Q000201:enzymology / Q000254:growth & development*; D008297:Male; D051381:Rats; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors*; D014638:Vanadates; D014639:Vanadium / Q000494:pharmacology*
D014639:Vanadium; D014638:Vanadates; D000254:Sodium-Potassium-Exchanging ATPase