Malaria sporozoite penetration. A new approach by double staining.
To determine, whether a sporozoite is outside the hepatocyte membrane or internalized, a double staining test was carried out using, successively, antibody labeled with peroxidase and fluorescein. This test permits the quantification of sporozoite entry and outline sporozoite-hepatocyte interactions.
D000818:Animals; D002478:Cells, Cultured; D006801:Humans; D007124:Immunoenzyme Techniques; D008099:Liver / Q000469:parasitology*; D008288:Malaria / Q000469:parasitology*; D010961:Plasmodium / Q000502:physiology*