Effects of tunicamycin on the differentiation of F9 cells induced by either retinoic acid or retinoic acid and dibutyryl cyclic AMP.
Tunicamycin (0.5 micrograms/ml) inhibited differentiation of F9 cells treated either with retinoic acid or with retinoic acid and dibutyryl cyclic AMP, as monitored by the activity of alkaline phosphatase and expression of cytokeratins. On the other hand, the pattern of the polysaccharide chain synthesis changed drastically with the treatment irrespective of the presence of tunicamycin. Therefore, phenotypes induced with retinoic acid are dissociated into two categories, one that is directly induced by the drug and the other that is induced indirectly by a mechanism in which glycoproteins play a role.
D000818:Animals; D003994:Bucladesine / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D002999:Clone Cells; D014411:Neoplastic Stem Cells / Q000166:cytology*; D011134:Polysaccharides / Q000096:biosynthesis; D014212:Tretinoin / Q000494:pharmacology*; D014415:Tunicamycin / Q000494:pharmacology*
D011134:Polysaccharides; D014415:Tunicamycin; D014212:Tretinoin; D003994:Bucladesine