Externally applied adenosine-5'-triphosphate causes inositol triphosphate accumulation in cultured chick myotubes.
In striated muscle, adenosine-5'-triphosphate (ATP) potentiates the responses to acetylcholine. The underlying biochemical events are unknown. Here we report that ATP, externally applied to chick myotubes, induces a rapid, dose-dependent accumulation of intracellular inositol triphosphate which is correlated with a decrease in phosphatidyl 4,5-bisphosphate. Adenosine-5'-diphosphate, adenosine-5'-monophosphate and adenosine are less potent while beta, gamma-imido ATP is equipotent motoneurons and/or skeletal muscle controls the activation of a polyphosphoinositide phosphodiesterase via a cell membrane P2-purinoceptor, thus modulating skeletal muscle responses to transmitter release.
D000109:Acetylcholine / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000818:Animals; D002478:Cells, Cultured; D002645:Chickens; D015544:Inositol 1,4,5-Trisphosphate; D007295:Inositol Phosphates / Q000096:biosynthesis*; D009132:Muscles / Q000187:drug effects* / Q000378:metabolism; D018129:Phosphatidylinositol Phosphates; D010716:Phosphatidylinositols / Q000378:metabolism*; D011983:Receptors, Purinergic / Q000187:drug effects; D013403:Sugar Phosphates / Q000096:biosynthesis*
D007295:Inositol Phosphates; D018129:Phosphatidylinositol Phosphates; D010716:Phosphatidylinositols; D011983:Receptors, Purinergic; D013403:Sugar Phosphates; D015544:Inositol 1,4,5-Trisphosphate; D000255:Adenosine Triphosphate; D000109:Acetylcholine