title: A PMSG/GnRH method for the superovulation of the monovulatory brush-tailed possum (Trichosurus vulpecula).
abstract: Optimal ovarian stimulation with minimal degenerative changes was achieved in brush-tailed possums 3 days after a subcutaneous injection of 10 i.u. PMSG. This treatment alone did not result in ovulation and only rarely in oocyte maturation. Ovulation of 8-24 mature oocytes occurred when on the 3rd day after 10 i.u. PMSG the female received 3 intramuscular injections of 50 micrograms synthetic gonadotrophin-releasing hormone (GnRH) 90 min apart. Superovulation was achieved in immature, cycling, pregnant and lactating females, but not in animals in the preovulatory phase of a natural oestrous cycle.
mesh_terms: D000818:Animals; D005260:Female; D006064:Gonadotropins, Equine / Q000494:pharmacology*; D008394:Marsupialia / Q000502:physiology*; D008854:Microscopy, Electron; D009865:Oocytes / Q000648:ultrastructure; D010053:Ovary / Q000648:ultrastructure; D010060:Ovulation; D010906:Pituitary Hormone-Releasing Hormones / Q000494:pharmacology*; D011247:Pregnancy; D013268:Stimulation, Chemical; D013480:Superovulation
chemical_list: D006064:Gonadotropins, Equine; D010906:Pituitary Hormone-Releasing Hormones