Induced recombination and reversion of the CDC8 gene in relation to the cell cycle of yeast.
The induction of mitotic recombination in the CDC8 locus was studied in a diploid strain heteroallelic for cdc8 mutations (cdc8-1/cdc8-3); mitotic reversion was studied in strains cdc8-1/cdc8-1 and cdc8-3/cdc8-3. Conversion and reversion did not occur in those cells blocked at the S stage of the cell cycle by exposure to a nonpermissive temperature. In stationary phase cells irradiated just prior to exposure to temperature stress, the induction of recombinants was rather low and the induction of revertants was minimal. Conversely, a significant induction of cdc+ occurred in logarithmic phase cells subjected to the same treatment. Irradiation of synchronously dividing cultures revealed that intragenic recombination occurs at all three stages of the cell cycle-G1, S and G2. It was also found that UV-induced gene reversion can occur during the S and G2 stages, but not during the G1 stage of the cell cycle.
D000483:Alleles; D002453:Cell Cycle / Q000528:radiation effects; D004261:DNA Replication / Q000528:radiation effects; D004271:DNA, Fungal / Q000378:metabolism / Q000528:radiation effects; D005800:Genes, Fungal / Q000528:radiation effects; D009154:Mutation / Q000528:radiation effects; D011995:Recombination, Genetic / Q000528:radiation effects; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics* / Q000528:radiation effects; D013045:Species Specificity; D013696:Temperature; D014466:Ultraviolet Rays
D004271:DNA, Fungal