[Sedimentation characteristics of virion RNA of Machupo virus reproducing in the presence of actinomycin D].
Actinomycin D treatment (0.005-05 g/ml) of Vero and BHK-21 cells infected with Machupo virus suppressed the synthesis of ribosomal RNAs but did not affect the production of infectious Machupo virus. Virion RNAs contained 3 high molecular weight RNA species: 28-31 S, 22-24 S and 18 S. In the presence of actinomycin D [3H]-uridine incorporated only in 30-31 S and 22-24 S RNA species. The data are supported by previous results which show that Machupo virus genome contains two RNA species: "large" (30-31 S) and "small" (22-24 S).
D000818:Animals; D001116:Arenaviridae / Q000032:analysis*; D006477:Arenaviruses, New World / Q000032:analysis* / Q000502:physiology; D002499:Centrifugation, Density Gradient; D006224:Cricetinae; D003609:Dactinomycin / Q000494:pharmacology*; D012367:RNA, Viral / Q000032:analysis* / Q000096:biosynthesis; D014709:Vero Cells; D014771:Virion / Q000032:analysis*; D014779:Virus Replication / Q000187:drug effects*
D012367:RNA, Viral; D003609:Dactinomycin
Sedimentatsionnaia kharakteristika virionnykh RNK virusa Machupo, reprodutsiruiushchegosia v prisutstvii aktinomitsina D.