Transfer of Drosophila melanogaster transponsable genetic element mdg-4 into plant cells.
The copia-like element mdg-4, as a component of the Drosophila genomic fragment Dm111, was cloned into the vector pIB16. The chimaeric plasmid pIB16 [Dm111] was used to transform tobacco cells as a cointegrate with pTiC58 (Agrobacterium tumefaciens), pTiC58::pIB16 [Dm111]. The growth properties of primary crown-gall tumours were followed, and the DNA of one Nicotiana tabacum transformant was further analysed. The DNA/DNA molecular hybridization of digests of genomic DNA with 32P-labelled pDm111 probe demonstrated full-length insertion of Dm111 into N. tabacum genome. We were not able to detect any common sequence with Dm111 in untransformed tobacco cells.
D000818:Animals; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004251:DNA Transposable Elements; D015245:Deoxyribonuclease BamHI; D004331:Drosophila melanogaster / Q000235:genetics*; D010944:Plants / Q000235:genetics*; D010957:Plasmids
D004251:DNA Transposable Elements; D004262:DNA Restriction Enzymes; D015245:Deoxyribonuclease BamHI