Enhanced activity of mouse peritoneal cells after aclacinomycin administration.
We have investigated the activation of mouse peritoneal macrophages by injection of aclacinomycin (ACM). Macrophages from ACM-treated mice have an increased phagocytic activity as measured by Candida ingestion. The microbicidal activity indirectly evaluated by chemiluminescence and superoxide determination in response to stimulation with zymosan and 4 beta-phorbol-12-myristate-13 alpha-acetate is also greater in the cells from treated mice. Direct measurement of the cytostatic function, and of in vitro and in vivo cytotoxicity shows comparable significant increases against L1210 or P815 target cells. The enhanced antitumoral activity could not be attributed to the residual presence of ACM in the peritoneal cells since no drug was detected by high-performance liquid chromatography and since their freeze-thaw lysates incubated with P815 cells did not modify the growth of tumor cells as measured by [3H]-thymidine incorporation. We also checked that the presence of ACM did not influence the intensity of the chemiluminescence. In all tests performed, only i.p. ACM administration could stimulate the peritoneal cells. Since the doses of ACM inducing an increase in macrophage activity are effective on the survival of tumor-bearing mice, the participation of this mechanism in tumor control might be suggested.
D015250:Aclarubicin / Q000031:analogs & derivatives*; D000818:Animals; D003602:Cytotoxicity, Immunologic; D005609:Free Radicals; D008163:Luminescent Measurements; D008262:Macrophage Activation / Q000187:drug effects; D008264:Macrophages / Q000276:immunology / Q000502:physiology*; D051379:Mice; D009279:Naphthacenes / Q000494:pharmacology; D009374:Neoplasms, Experimental / Q000276:immunology; D010529:Peritoneal Cavity / Q000166:cytology*; D010587:Phagocytosis; D013481:Superoxides / Q000378:metabolism; D013997:Time Factors
D005609:Free Radicals; D009279:Naphthacenes; C011157:aclacinomycins; D013481:Superoxides; D015250:Aclarubicin