Lorist6, a cosmid vector with BamHI, NotI, ScaI and HindIII cloning sites and altered neomycin phosphotransferase gene expression.
Four new features have been included in a phage-lambda-replicon-based cosmid vector, Lorist6. A ScaI restriction site allows insertion of blunt-ended DNA, partially digested with AluI or any other restriction enzyme which creates blunt ends after cleavage. NotI and BamHI sites enable construction of libraries using double digestion with NotI + Sau3AI. The promoter of the neo gene has been substituted by a promoter derived from the tet gene of pBR322, increasing expression of the resistance gene product. A transcriptional terminator has been placed downstream from the neo gene to prevent transcription into insert DNAs.
D003001:Cloning, Molecular / Q000379:methods*; D003360:Cosmids; D004262:DNA Restriction Enzymes; D005786:Gene Expression Regulation; D005822:Genetic Vectors; D019868:Kanamycin Kinase; D010770:Phosphotransferases / Q000235:genetics*; D011401:Promoter Regions, Genetic
D010770:Phosphotransferases; D019868:Kanamycin Kinase; D004262:DNA Restriction Enzymes