Activity of ATP synthetase complex after low temperature treatment or freeze-drying of mitochondria isolated from skeletal muscles.
The influence of freezing, thawing, or freeze-drying on ATP synthetase complex of isolated skeletal muscle mitochondria was studied. Cooling to -60 or to -196 degrees C and rapid thawing did not change activity significantly. Slow warming stimulated the release of latent ATP-ase activity and decreased ATP synthesis. These changes were more pronounced after freeze-drying.
D025181:ATP Synthetase Complexes; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D003080:Cold Temperature; D004140:Dinitrophenols / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D005612:Freeze Drying; D007425:Intracellular Membranes / Q000201:enzymology / Q000648:ultrastructure; D008931:Mitochondria, Muscle / Q000201:enzymology* / Q000648:ultrastructure; D009097:Multienzyme Complexes / Q000378:metabolism*; D010770:Phosphotransferases / Q000378:metabolism*; D011309:Preservation, Biological / Q000379:methods*; D013552:Swine
D004140:Dinitrophenols; D009097:Multienzyme Complexes; D000255:Adenosine Triphosphate; D010770:Phosphotransferases; D025181:ATP Synthetase Complexes