Isoproterenol treatment causes cytoskeletal reorganization in chicken lens fiber cells.
Cytoskeletal organization in organ cultured embryonic chicken lens was affected by isoproterenol treatment. Immunofluorescent localization of a 49 kD fiber cell-specific cytoskeletal protein showed a cytoplasmic and membrane localization in control lenses. Following isoproterenol treatment, fluorescence was predominantly membrane-associated. Changes in 49 kD distribution as determined by immunofluorescence occurred within minutes of isoproterenol application and were completely blocked by propranolol pretreatment. These results suggest that beta-adrenergic stimulation influences the apparent localization of a fiber cell-specific cytoskeletal protein.
D000818:Animals; D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure; D002642:Chick Embryo; D003598:Cytoskeletal Proteins / Q000378:metabolism; D003599:Cytoskeleton / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure*; D004305:Dose-Response Relationship, Drug; D005455:Fluorescent Antibody Technique; D007545:Isoproterenol / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D007908:Lens, Crystalline / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure*; D008970:Molecular Weight; D011433:Propranolol / Q000494:pharmacology; D013997:Time Factors
D003598:Cytoskeletal Proteins; D011433:Propranolol; D007545:Isoproterenol