title: A necrotizing pneumonia in lambs caused by pseudorabies virus (Aujesky's disease virus).
abstract: An outbreak of pseudorabies occurred in sheep housed with swine in the same building. Although the sheep and swine were not in physical contact, the lambs and ewes were exposed to air from the sows' section. Three dead lambs were submitted to the Iowa State University Veterinary Diagnostic Laboratory for necropsy. Grossly there were pulmonary congestion and multifocal pulmonary hemorrhages. Microscopic lesions were severe acute multifocal necrotizing bronchopneumonia with necrotizing vasculitis and intranuclear inclusion bodies within the neurons of the parabronchial ganglia. Bacterial cultures were negative for pathogenic agents; pseudorabies virus was isolated from ovine brain tissue. Viral antigen was demonstrated in the neurons of the parabronchial ganglia by immunoperoxidase staining. Electron microscopy revealed nucleocapsids in the parabronchial ganglionic neurons which contained basophilic intranuclear inclusion bodies. Viral DNA prepared from the ovine pseudorabies virus isolate was found by restriction endonuclease analysis to be related to the Indiana Funkhauser strain of pseudorabies virus.
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D001921:Brain / Q000382:microbiology; D004279:DNA, Viral / Q000032:analysis; D004196:Disease Outbreaks / Q000662:veterinary*; D005260:Female; D011558:Herpesvirus 1, Suid / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D007124:Immunoenzyme Techniques; D008168:Lung / Q000473:pathology* / Q000648:ultrastructure; D008854:Microscopy, Electron; D009474:Neurons / Q000382:microbiology; D011024:Pneumonia, Viral / Q000453:epidemiology / Q000473:pathology / Q000635:transmission / Q000662:veterinary*; D011557:Pseudorabies / Q000453:epidemiology / Q000473:pathology* / Q000635:transmission; D012756:Sheep; D012757:Sheep Diseases / Q000453:epidemiology / Q000473:pathology* / Q000635:transmission; D013552:Swine; D013553:Swine Diseases / Q000635:transmission
chemical_list: D000956:Antigens, Viral; D004279:DNA, Viral