Expression of the granulocyte/macrophage colony-stimulating factor gene in leukemic blast cells from patients with acute non-lymphocytic leukemia.
Since granulocyte/macrophage colony-stimulating factor (GM-CSF) has been reported to stimulate the proliferation of clonogenic leukemia cells from patients with acute non-lymphocytic leukemia in vitro, the expression of the GM-CSF gene in primary human leukemic blast cells was studied. T-cell-depleted mononuclear cells in freshly drawn peripheral blood from patients with acute non-lymphocytic leukemia (ANLL) were subjected to the study. The expression of the GM-CSF gene was detected by Northern blotting analysis using [32P]GM-CSF as a probe. The GM-CSF gene was expressed in two out of five cases examined. In both of the patients who showed GM-CSF expression, remission could not be achieved.
D000208:Acute Disease; D003115:Colony-Stimulating Factors / Q000235:genetics*; D005796:Genes; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D006098:Granulocytes; D006133:Growth Substances / Q000235:genetics*; D006801:Humans; D007938:Leukemia / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D008264:Macrophages; D012333:RNA, Messenger / Q000235:genetics*
D003115:Colony-Stimulating Factors; D006133:Growth Substances; D012333:RNA, Messenger; D016178:Granulocyte-Macrophage Colony-Stimulating Factor