title: An improved procedure for shipboard enumeration of faecal indicator bacteria in marine sediments from sewage sludge disposal areas.
abstract: An improved membrane filtration procedure for use on board ship to enumerate Escherichia coli and Group D faecal streptococci in marine sediments is described. Ultrasonication extraction combined with resuscitation of sublethally-injured cells yielded significantly higher counts of E. coli than sediments shaken by hand. Counts of E. coli were also higher on mFC agar (without rosalic acid) after a period of resuscitation on tryptone-soy agar supplemented with 0.1% yeast extract than on a 4% Teepol-lactose medium. Ultrasonication of sediments made no significant difference to counts of Group D faecal streptococci on KF-streptococcus agar. These improved isolation procedures allowed better discrimination of the area affected by sewage sludge at a disposal site off the northeast coast of England.
mesh_terms: D015169:Colony Count, Microbial; D004926:Escherichia coli / Q000254:growth & development / Q000302:isolation & purification*; D005243:Feces / Q000382:microbiology; D005374:Filtration; D006801:Humans; D012623:Seawater; D012722:Sewage; D012767:Ships; D013291:Streptococcus / Q000254:growth & development / Q000302:isolation & purification*; D014465:Ultrasonics; D014871:Water Microbiology
chemical_list: D012722:Sewage