[Translocation of ampicillin transposon Tn1 in Escherichia coli cells during sexual reproduction].
The efficiency of Tn1 transposition has been shown to increase considerably in course of bacterial conjugation. Usually, the frequency of Tn1 transposition from plasmid pSA2001, a derivative of RP4, into the chromosome never exceeded 0.1% per cell. Percentage of His+ transconjugants, marked by transposon Tn1 during conjugation between Hfr donor, carrying plasmid pSA2001, and auxotrophic recipient, was about 30%. Transposon Tn1 transfer into the recipient cells does not depend on the recA+ gene function in donor cells or on conjugative transfer of plasmid pSA2001. The transfer requires the recA+ gene function in recipients as well as the Hfr function in donor cells. Southern's blot-hybridization revealed the insertion of transposon Tn1 into the different sites of the chromosome of His+ transconjugants. The transposon inserted during conjugation retains the ability to potential further translocation into new sites on the chromosomal DNA.
D000667:Ampicillin / Q000494:pharmacology*; D003227:Conjugation, Genetic; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics*; D005819:Genetic Markers; D010403:Penicillin Resistance; D010957:Plasmids; D014178:Translocation, Genetic
D004251:DNA Transposable Elements; D005819:Genetic Markers; D000667:Ampicillin
Translokatsiia ampitsillinovogo transpozona Tn1 v kletkakh Escherichia coli pri polovom protsesse.