title: Expression of nuclear lamins during mouse preimplantation development.
abstract: The expression of nuclear lamins during mouse preimplantation development was studied by immunofluorescence, immunoblotting and immunoprecipitation. Two sera were used, specific either for lamin B or lamins A and C. Both sera gave a positive staining of the nuclear periphery throughout preimplantation development (fertilized eggs to late blastocysts). Immunoblots revealed that the three lamins were present in eggs and blastocysts. However, lamin A from eggs was found to have a higher apparent Mr than lamin A from blastocysts and other mouse cells. Using immunoprecipitation, synthesis of lamin A was detected in eggs while synthesis of lamin B was detected in 8-cell embryos and blastocysts, indicating that at least some of the lamins used during early development do not come from a store in the egg. These results are discussed in relation to the possible role of lamins during cell differentiation.
mesh_terms: D000818:Animals; D047108:Embryonic Development; D005260:Female; D005455:Fluorescent Antibody Technique; D007106:Immune Sera; D034904:Lamin Type A; D034921:Lamin Type B; D034882:Lamins; D051379:Mice; D009687:Nuclear Proteins / Q000032:analysis* / Q000276:immunology; D011247:Pregnancy
chemical_list: D007106:Immune Sera; D034904:Lamin Type A; D034921:Lamin Type B; D034882:Lamins; D009687:Nuclear Proteins