Localization of glycine uptake and receptors in the cat retina.
Immunocytochemistry using a monoclonal antibody against glycine receptors revealed that these receptors in cat retina are confined to the inner plexiform layer (IPL). The outer half of that layer showed strong patchy labelling with some indication of two bands corresponding to the on- and off-sublamina. High-affinity uptake of [3H]glycine followed by autoradiography labelled amacrine cells, bipolar cells and the outer portion of the IPL. Silver grains in the IPL were patchily distributed. These results indicate in the cat retina a close match between presynaptic glycinergic elements labelled by high-affinity uptake and the postsynaptic receptor sites for glycine revealed by immunocytochemistry.
D000818:Animals; D001345:Autoradiography; D002415:Cats; D005455:Fluorescent Antibody Technique; D005998:Glycine / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism; D018009:Receptors, Glycine; D017981:Receptors, Neurotransmitter / Q000378:metabolism*; D012160:Retina / Q000378:metabolism*
D018009:Receptors, Glycine; D017981:Receptors, Neurotransmitter; D005998:Glycine