[Cell kinetics of oligodendroglioma and oligo-astrocytoma--Ki-67 PaP study].
In order to determine the principal histologic features in distinguishing the "anaplastic" from the "well differentiated" oligodendroglioma and mixed glioma (oligo-astrocytoma), correlations between the growth fraction, and histologic grade and 6 histologic variables (vascular proliferation, cytologic pleomorphism, calcification, necrosis, cellularity, mitotic index) were studied in 24 patients. The growth fraction was calculated as the percentage of Ki-67-immunostained nuclei in frozen sections using Ki-67 monoclonal antibody. 6 histologic variables were checked with HE stain of paraffin-embedded tissue sections. The growth fraction was in general agreement with the histologic grade, in order of decreasing mean percentage, ranging from 11.1% (anaplastic mixed glioma) to 5.3% (anaplastic oligodendroglioma), 2.8% (isomorphic oligo.) and 1.9% (mixed, grade II). There was a significant association between the growth fraction and 3 histologic variables (vascular proliferation, cytologic pleomorphism, mitotic index) out of 6. Thus, even though there exist some gaps between these parameters in paraffin-embedded tissues and those in frozen sections, the percentage of Ki-67-immunostained nuclei is likely to be very valuable as a supplement information of histological grading and a prognostic indicator.
D000328:Adult; D000911:Antibodies, Monoclonal; D001932:Brain Neoplasms / Q000473:pathology*; D002453:Cell Cycle; D002648:Child; D005260:Female; D005910:Glioma / Q000473:pathology*; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D008875:Middle Aged; D009836:Oligodendroglia / Q000473:pathology; D009837:Oligodendroglioma / Q000473:pathology*
D000911:Antibodies, Monoclonal