Role of actin polymerization in monocyte phagocytosis of yeast cells effect of cytochalasin B.
The role of the cortical actomyosin-like contractile system in monocyte phagocytosis was studied by means of inhibition with cytochalasin B. Monocyte phagocytosis of yeast cells was assayed with a fluorescence extinction technique of surface-located phagocytosis which distinguishes between adherence and ingestion phases of total phagocytosis. Cytochalasin B, 10 micrograms/ml, inhibited monocyte phagocytosis by 20-30%. The cytochalasin inhibition was significant and restricted to the ingestion phase. The finding supported the hypothesis that the contractile structures of the cell have a contributory role in IgG-mediated monocyte phagocytosis on a surface. It is suggested that the cytochalasin effect reflected the role of chemotaxis in monocyte phagocytosis assayed by the present technique.
D000199:Actins / Q000378:metabolism*; D002448:Cell Adhesion; D002633:Chemotaxis; D003470:Culture Media; D003571:Cytochalasin B / Q000494:pharmacology*; D004121:Dimethyl Sulfoxide; D005898:Glass; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000276:immunology*; D010587:Phagocytosis / Q000187:drug effects*; D011108:Polymers; D012441:Saccharomyces cerevisiae / Q000276:immunology
D000199:Actins; D003470:Culture Media; D011108:Polymers; D003571:Cytochalasin B; D004121:Dimethyl Sulfoxide