Development of a plaque assay for a cytopathic, rapidly replicating isolate of hepatitis A virus.
Most hepatitis A virus (HAV) replication in cell culture has been reported to be nonlytic and relatively slow. A rapidly replicating isolate of strain HM-175 from persistently infected, serially passed cell cultures (pHM-175) was found to induce a cytopathic effect. This observation allowed the development of a classic plaque assay for pHM-175 in FRhK-4 cells. The plaques were neutralized by polyclonal and monoclonal antisera to HAV.
D000818:Animals; D002460:Cell Line; D002470:Cell Survival; D003588:Cytopathogenic Effect, Viral; D035922:Hepatitis A Antibodies; D006508:Hepatitis Antibodies / Q000276:immunology; D006507:Hepatovirus / Q000254:growth & development* / Q000276:immunology / Q000502:physiology; D009500:Neutralization Tests; D010948:Viral Plaque Assay
D035922:Hepatitis A Antibodies; D006508:Hepatitis Antibodies