In situ molecular hybridization for detection of Aleutian mink disease parvovirus DNA by using strand-specific probes: identification of target cells for viral replication in cell cultures and in mink kits with virus-induced interstitial pneumonia.
Strand-specific hybridization probes were utilized in in situ molecular hybridization specifically to localize replicative form DNA of Aleutian mink disease parvovirus (ADV). Throughout in vitro infection, duplex replicative form DNA of ADV was located in the cell nuclei. Single-stranded virion DNA and capsid proteins were present in the nuclei early in infection, but were later translocated to the cytoplasm. In neonatal mink, ADV causes acute interstitial pneumonia, and replicative forms of viral DNA were found predominantly in alveolar type II cells of the lung. Viral DNA was also found in other organs, but strand-specific probes made it possible to show that most of this DNA represented virus sequestration. In addition, glomerular immune complexes containing intact virions were detected, suggesting that ADV virions may have a role in the genesis of ADV-induced glomerulonephritis.
D000454:Aleutian Mink Disease Virus / Q000235:genetics* / Q000276:immunology; D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002467:Cell Nucleus / Q000382:microbiology; D002478:Cells, Cultured; D004279:DNA, Viral / Q000032:analysis*; D007668:Kidney / Q000382:microbiology; D008168:Lung / Q000382:microbiology; D008907:Mink / Q000382:microbiology*; D009693:Nucleic Acid Hybridization; D010321:Parvoviridae / Q000235:genetics*; D011024:Pneumonia, Viral / Q000382:microbiology / Q000662:veterinary*; D013045:Species Specificity; D014018:Tissue Distribution; D014779:Virus Replication
D000956:Antigens, Viral; D004279:DNA, Viral