Effect of ethanol on the glucose-induced movements of protons across the plasma membrane of Saccharomyces cerevisiae NCYC 431.
The study of glucose-induced proton fluxes in Saccharomyces cerevisiae NCYC 431 showed a decrease of proton net efflux by ethanol across the plasma membrane of energized cells. Furthermore a negative net proton efflux (an influx) occurred from a given ethanol concentration (between 1.3 and 1.5 M) whatever the experimental conditions used, thus allowing the definition of a nil-net exchange step where no net movement of protons across the plasma membrane could be observed. A new technique of ethanol tolerance determination in yeast based upon a correlation for the same ethanol concentration between both the collapse of the proton gradient and the growth cessation in cultures supplemented with ethanol after 8 h incubation was proposed. The defined method also showed a cumulated effect of temperature and ethanol on Saccharomyces cerevisiae NCYC 431.
D002462:Cell Membrane / Q000378:metabolism; D000431:Ethanol / Q000494:pharmacology*; D005947:Glucose / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D011522:Protons; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000378:metabolism*; D013696:Temperature
D011522:Protons; D000431:Ethanol; D005947:Glucose