Enhancement of [3H]clonidine binding to rat cerebral synaptic membranes by treatment with arachidonic acid, prostaglandin (PG) D2, PGE2 and PGF2 alpha.
Pretreatment of cerebral synaptic membranes with arachidonic acid (10 nM-1 microM) at 37 degrees C for 40 min caused a significant increase in [3H]clonidine binding but pretreatment at 4 degrees C did not. Scatchard analysis showed that 100 nM arachidonic acid pretreatment resulted in a significant elevation of the Bmax value of the low affinity site. PGD2, PGE2 and PGF2 alpha (10-100 microM) pretreatment also enhanced binding. These polyunsaturated fatty acids could be involved in regulation of the alpha 2-adrenoceptor binding in rat cerebral cortical membranes.
D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000494:pharmacology*; D002540:Cerebral Cortex / Q000378:metabolism*; D003000:Clonidine / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D011453:Prostaglandins / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D011942:Receptors, Adrenergic, alpha / Q000187:drug effects* / Q000378:metabolism; D013696:Temperature
D001095:Arachidonic Acids; D011453:Prostaglandins; D011942:Receptors, Adrenergic, alpha; D016718:Arachidonic Acid; D003000:Clonidine