title: Rous sarcoma virus mutant dlPA105 induces different transformed phenotypes in quail embryonic fibroblasts and neuroretina cells.
abstract: dlPA105 is a spontaneous variant of Rous sarcoma virus, subgroup E, which carries a deletion in the N-terminal portion of the v-src gene coding sequence. This virus was isolated on the basis of its ability to induce proliferation of quiescent quail neuroretina cells. The altered v-src gene encodes a phosphoprotein of 45,000 daltons which possesses tyrosine kinase activity. DNA sequencing of the mutant v-src gene has shown that deletion extends from amino acid 33 to 126 of wild-type p60v-src. We investigated the tumorigenic and transforming properties of this mutant virus. dlPA105 induced fibrosarcomas in quails with an incidence identical to that induced by wild-type virus. Quail neuroretina cells infected with the mutant virus were morphologically transformed and formed colonies in soft agar. In contrast, dlPA105 induced only limited morphological alterations in quail fibroblasts and was defective in promoting anchorage-independent growth of these cells. Synthesis and tyrosine kinase activity of the mutant p45v-src were similar in both cell types. These data indicate that the portion of the v-src protein deleted in p45v-src is dispensable for the mitogenic and tumorigenic properties of wild-type p60v-src, whereas it is required for in vitro transformation of fibroblasts. The ability of dlPA105 to induce different transformation phenotypes in quail fibroblasts and quail neuroretina cells is a property unique to this Rous sarcoma virus mutant and provides evidence for the existence of cell-type-specific response to v-src proteins.
mesh_terms: D000818:Animals; D001358:Avian Sarcoma Viruses / Q000502:physiology*; D002451:Cell Compartmentation; D002455:Cell Division; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D003370:Coturnix; D003599:Cytoskeleton / Q000648:ultrastructure; D009154:Mutation; D009856:Oncogene Proteins, Viral / Q000502:physiology*; D009857:Oncogenes; D010641:Phenotype; D010750:Phosphoproteins / Q000378:metabolism; D010960:Plasminogen Activators / Q000096:biosynthesis; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D012160:Retina / Q000166:cytology; D012513:Sarcoma, Experimental / Q000235:genetics* / Q000382:microbiology
chemical_list: D009856:Oncogene Proteins, Viral; D010750:Phosphoproteins; D011505:Protein-Tyrosine Kinases; D010960:Plasminogen Activators