title: Owl monkey astrocytoma cells in culture spontaneously produce infectious JC virus which demonstrates altered biological properties.
abstract: A tumor cell suspension of an explanted JC virus (JCV)-induced owl monkey glioblastoma was inoculated intracranially into four recipient juvenile owl monkeys. Twenty-eight months following inoculation one owl monkey developed a glioblastoma, which was explanted into tissue culture. DNA from both the tumor tissue and tumor cells in culture hybridized to a JCV DNA probe by Southern analysis, indicating that free, as well as integrated, viral DNA may be present. At the time of the second culture passage, viral JCV DNA was extracted from these cells and cloned into a plasmid vector. Nucleotide sequencing of the regulatory region of the cloned DNA demonstrated homology with the prototype Mad-1 strain of JCV and revealed a 19-base-pair deletion in the second 98-base-pair tandem repeat that eliminated a second TATA box. This deletion is characteristic of the Mad-4 strain of JCV, which is highly neurooncogenic. By the third culture passage, 100% of the cells were T-antigen positive. Approximately one-third of the cells in culture hybridized to a biotinylated JCV DNA probe when in situ hybridization was used, a technique that only detects high-copy-number of replicating viral sequences. By the culture passage 5 and continuing through culture passage 14, viable JC virions could be recovered. The T protein synthesized by this virus, now termed JCV-586, differed from both the Mad-1 and Mad-4 strains in that it formed a stable complex with the cellular p53 protein in the tumor cells. Also, the JCV-586 T protein reacted to several monoclonal antibodies made to the simian virus 40 T protein that were not recognized by either the Mad-1 or Mad-4 strains.
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000957:Antigens, Viral, Tumor / Q000032:analysis; D001029:Aotus trivirgatus; D001254:Astrocytoma / Q000382:microbiology*; D001932:Brain Neoplasms / Q000382:microbiology*; D004279:DNA, Viral / Q000032:analysis; D005910:Glioma / Q000382:microbiology*; D007577:JC Virus / Q000235:genetics* / Q000254:growth & development; D009368:Neoplasm Transplantation; D009693:Nucleic Acid Hybridization; D011120:Polyomavirus / Q000235:genetics*; D014779:Virus Replication
chemical_list: D000911:Antibodies, Monoclonal; D000957:Antigens, Viral, Tumor; D004279:DNA, Viral