Detection of circulating antibodies against c-myc protein in cancer patient sera.
We have partially purified an archaebacterial protein of 84 kD which shares common epitopes with the human c-myc protein as shown by its cross-reactivity with a commercialized anti-human c-myc antiserum. An antiserum raised against the 84 kD protein recognizes a 60 kD protein from HL-60 nuclei. This protein is also recognized by the anti-human c-myc antiserum. Using this archaebacterial protein as antigen for Western blot analysis, we found that the human c-myc oncogene product could be immunogenic and that it is possible, in some spontaneously occurring human tumours, to detect antibodies against the c-myc gene product in the serum of cancer patients.
D000912:Antibodies, Neoplasm / Q000032:analysis*; D001667:Binding, Competitive; D002848:Chromatography, DEAE-Cellulose; D004591:Electrophoresis, Polyacrylamide Gel; D006217:Halobacterium / Q000032:analysis; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D008970:Molecular Weight; D009369:Neoplasms / Q000276:immunology*; D011518:Proto-Oncogene Proteins / Q000276:immunology* / Q000302:isolation & purification; D016271:Proto-Oncogene Proteins c-myc
D000912:Antibodies, Neoplasm; D007106:Immune Sera; D011518:Proto-Oncogene Proteins; D016271:Proto-Oncogene Proteins c-myc