Activity of a Streptomyces transcriptional terminator in Escherichia coli.
A 205bp DNA fragment from the Streptomyces multi-copy plasmid pIJ101 has in vivo terminator activity both in Streptomyces lividans and in Escherichia coli. Termination of RNA synthesis, detected by high-resolution S1 nuclease mapping, occurs at precisely the same nucleotides in both organisms. This suggests that the E. coli RNA polymerase recognizes the same sequence elements and chooses the point(s) of termination in the same way as the corresponding S. lividans enzyme.
D002701:Chloramphenicol / Q000494:pharmacology; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D004352:Drug Resistance, Microbial; D004720:Endonucleases; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D005798:Genes, Bacterial; D005809:Genes, Regulator; D007612:Kanamycin / Q000494:pharmacology; D015719:Single-Strand Specific DNA and RNA Endonucleases; D013302:Streptomyces / Q000187:drug effects / Q000235:genetics*; D013728:Terminator Regions, Genetic; D014158:Transcription, Genetic
D007612:Kanamycin; D002701:Chloramphenicol; D012321:DNA-Directed RNA Polymerases; D004720:Endonucleases; D015719:Single-Strand Specific DNA and RNA Endonucleases