A cytomegalovirus protein with properties of herpes simplex virus ICP8: partial purification of the polypeptide and map position of the gene.
We demonstrated the presence of a single-stranded DNA-binding protein in human cytomegalovirus (CMV)-infected cells with properties analogous to those of herpes simplex virus (HSV) ICP8. Using monoclonal antibody specific for the CMV protein, we analyzed its fluorescence pattern and time of synthesis, mapped the gene encoding it by using a lambda gt11 library of CMV DNA fragments, and monitored its purification by phosphocellulose and DNA-Sepharose chromatography. In all characteristics we examined, the CMV protein behaved analogously to HSV ICP8. Our results are consistent with a functional role of CMV ICP8 in viral replication that is similar to that of HSV ICP8 and with the evolutionary conservation of the gene of interest in two divergent herpesviruses.
D000595:Amino Acid Sequence; D002460:Cell Line; D003587:Cytomegalovirus / Q000032:analysis / Q000235:genetics*; D004277:DNA, Single-Stranded / Q000378:metabolism; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D004268:DNA-Binding Proteins / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D005455:Fluorescent Antibody Technique; D005814:Genes, Viral; D006801:Humans; D007118:Immunoassay; D007700:Kinetics; D012367:RNA, Viral / Q000032:analysis; D012689:Sequence Homology, Nucleic Acid; D018139:Simplexvirus / Q000235:genetics*; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification
D004277:DNA, Single-Stranded; D004279:DNA, Viral; D004268:DNA-Binding Proteins; C045462:ICP8 protein, Simplexvirus; D012367:RNA, Viral; D014764:Viral Proteins