[Transfer of "artificial transposons" constructed on the basis of insertion element IS1].
Terminal inverted repeats of the insertion element IS1 were synthesized chemically and plasmids containing these sequences flanking kanamycin-resistance gene in different combinations were constructed. Further incorporation of a whole-sized copy of the IS1 into such plasmids caused in some cases the autonomous transfer of Km-resistance from plasmid to bacteriophage lambda DNA. The transposition of the Km-resistance gene was only observed in those cases when the gene was enclosed between IS1 copy and one of the terminal repeats. The data obtained are discussed with regard to the evolution of bacterial transposons.
D010582:Bacteriophage lambda / Q000235:genetics; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics*; D005813:Genes, Synthetic; D005822:Genetic Vectors; D010957:Plasmids; D012091:Repetitive Sequences, Nucleic Acid
D004251:DNA Transposable Elements
Peremeshchenie "iskusstvennykh transpozonov", skonstruirovannykh na osnove insertsionnogo élementa IS1.