Identification of a new 84/82 kDa calmodulin-binding protein, which also interacts with actin filaments, tubulin and spectrin, as synapsin I.
A new 84/82 kDa calmodulin-binding protein, which also interacts with actin filaments, tubulin and spectrin, was purified from the bovine synaptosomal membrane. The binding of calmodulin to this protein was Ca2+-dependent, and was inhibited by trifluoperazine, the association constant being calculated to be 2.2 X 10(6) M-1. Maximally, 1 mol of calmodulin bound to 1 mol of the purified protein. This protein was phosphorylated by both kinase II (Ca2+- and calmodulin-dependent kinase) and cyclic AMP-dependent kinase. In addition, antibody against this protein was demonstrated to have an immunological crossreactivity with synapsin I in the synaptosomal membrane.
D000199:Actins / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002147:Calmodulin / Q000378:metabolism; D002148:Calmodulin-Binding Proteins / Q000032:analysis*; D002417:Cattle; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D007703:Peptidyl-Dipeptidase A / Q000378:metabolism; D011494:Protein Kinases / Q000378:metabolism; D013049:Spectrin / Q000378:metabolism*; D016704:Synapsins; D013570:Synaptic Membranes / Q000032:analysis; D014268:Trifluoperazine / Q000494:pharmacology; D014404:Tubulin / Q000378:metabolism*
D000199:Actins; D002147:Calmodulin; D002148:Calmodulin-Binding Proteins; D009419:Nerve Tissue Proteins; D016704:Synapsins; D014404:Tubulin; D013049:Spectrin; D014268:Trifluoperazine; D011494:Protein Kinases; D007703:Peptidyl-Dipeptidase A; D002118:Calcium