[2 types of molecular structure (composition) of a genome in one species of transposable bacteriophages of Pseudomonas aeruginosa].
Comparison of heteroduplexes (HD) between DNAs of different transposable phages of Pseudomonas aeruginosa belonging to two previously described subgroups (D3112 and B3) revealed two types of structure (composition) of the bacteriophages, designated "type A" and "type B". The properties of genome structure of type A (phages of D3112 subgroup) are as follows: high level of conservation (up to 70% of genomes of different phages are represented as blocks of homologous DNA sequences); substitutions in genomes revealed as nonhomology regions in HD are, as a rule, small and located in certain sites; the distribution of the nonhomologous regions in HD of these phages is highly reproducible in independent experiments. Bacteriophages of subgroup B3 have genomes of type B: only a small part (approx. 30%) of genomes retain homology general for all of the phages; the nonhomologous regions are distributed in a large number of sites in HD; the sizes of nonhomologous regions are substantially larger than for the phages of subgroup D3112; distribution of the regions in HD is highly variable, which is characteristic of DNAs with partial homology. There is no difference between genomes of types A and B in G + C content (approx. 61-63%). Viable recombinants can be formed in crosses between phages of different genome types not only in regions with earlier revealed large DNA/DNA homology (right ends of genomes), but also in central portions of the genomes. Nevertheless, functional incompatibility of some regions of phage genomes of types A and B was demonstrated.
D001435:Bacteriophages / Q000235:genetics*; D001482:Base Composition; D005075:Biological Evolution; D004251:DNA Transposable Elements; D005814:Genes, Viral; D011550:Pseudomonas aeruginosa / Q000235:genetics; D013045:Species Specificity
D004251:DNA Transposable Elements
Dva tipa molekuliarnoĭ struktury (sostava) genoma v predelakh odnogo vida bakteropfagov-transpozonov Pseudomonas aeruginosa.