[Demonstration of the expression of bovine leukemogenic virus (BLV) in sheep lymphocytes by an immunofluorescence technic using monoclonal antibodies].
An indirect immunofluorescence (IF) test was developed to detect bovine leukemia virus (BLV) antigen expression in infected sheep lymphocytes, using monoclonal antibodies anti BLV-major envelope glycoprotein gp51. Peripheral blood lymphocytes were cultivated for 48 h in presence of phytohemagglutinin (PHA) (50 micrograms/ml), and then fixed with acetone. The cells were assayed for the IF test. All experimentally infected sheep were positive with this test.
D000818:Animals; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral / Q000032:analysis*; D002478:Cells, Cultured; D005455:Fluorescent Antibody Technique; D001909:Leukemia Virus, Bovine / Q000276:immunology / Q000302:isolation & purification*; D007942:Leukemia, Experimental / Q000175:diagnosis*; D008214:Lymphocytes / Q000276:immunology / Q000382:microbiology*; D008297:Male; D012190:Retroviridae / Q000302:isolation & purification*; D012756:Sheep; D014759:Viral Envelope Proteins / Q000276:immunology
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D014759:Viral Envelope Proteins
Mise en évidence de l'expression du virus leucémogène bovin (BLV) dans les lymphocytes de mouton par une technique d'immunofluorescence utilisant des anticorps monoclonaux.