Photosensitization of leukemic cells and normal bone marrow cells by 514 nm laser light and effects of laser light on migration inhibition and lymphokine response.
In a model for ex-vivo purging of bone marrow grafts, leukemic cells and normal bone marrow cells were treated with merocyanine 540 and exposed to 514 nm laser light. With this treatment, 99.9999% of leukemic cells were killed while 55% of the normal bone marrow cells survived. The deleterious effects of laser light alone in the absence of photosensitizer were not observed as determined by cell viability, cell migration, and response of target cells to human migration inhibition factor. These results indicate that laser light induced photodynamic therapy can be useful for ex-vivo autologous bone marrow purging without regard to the deleterious effects of laser light alone.
D001853:Bone Marrow / Q000187:drug effects; D001854:Bone Marrow Cells; D002460:Cell Line; D002464:Cell Migration Inhibition; D002470:Cell Survival; D006801:Humans; D007834:Lasers; D015470:Leukemia, Myeloid, Acute / Q000473:pathology*; D008263:Macrophage Migration-Inhibitory Factors / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects / Q000276:immunology*; D010778:Photochemotherapy; D011744:Pyrimidinones / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D008263:Macrophage Migration-Inhibitory Factors; D011744:Pyrimidinones; C003954:merocyanine dye