Molecular cloning of a recA-like gene from the cyanobacterium Anabaena variabilis.
A recA-like gene isolated from the cyanobacterium Anabaena variabilis was cloned and partially characterized. When introduced into Escherichia coli recA mutants, the 7.5-kilobase-pair plasmid-borne DNA insert restored resistance to methyl methanesulfonate and UV irradiation, as well as recombination proficiency when measured by Hfr-mediated conjugation. The cyanobacterial recA gene restored spontaneous but not mitomycin C-induced prophage production. Restriction analysis and subcloning yielded a 1.5-kilobase-pair Sau3A fragment which also restored methylmethane sulfonate resistance and coded for a 38- to 40-kilodalton polypeptide when expressed in an in vitro transcription-translation system.
D003001:Cloning, Molecular; D003227:Conjugation, Genetic / Q000187:drug effects; D000458:Cyanobacteria / Q000235:genetics*; D004260:DNA Repair; D004262:DNA Restriction Enzymes; D005816:Genetic Complementation Test; D008242:Lysogeny; D008741:Methyl Methanesulfonate / Q000494:pharmacology; D009693:Nucleic Acid Hybridization; D014176:Protein Biosynthesis; D011938:Rec A Recombinases / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic; D014466:Ultraviolet Rays
D008741:Methyl Methanesulfonate; D011938:Rec A Recombinases; D004262:DNA Restriction Enzymes