A temperature-sensitive mutant of Abelson murine leukemia virus confers inducibility of IgM expression to transformed lymphoid cells.
Lymphoid cell lines were isolated that were inducible for the expression of surface immunoglobulin by shift from 35.5 to 39.5 degrees C after infection of mouse bone marrow cells with a mutagen-treated Abelson murine leukemia virus. Virus produced by one of the cell lines (ts49) transmitted the temperature-sensitive phenotype to new lymphoid transformants as well as to NIH/3T3 cells. In addition, the tyrosine autophosphorylating activity of the p120gag-abl protein synthesized in ts49-transformed cells was found to be temperature-sensitive. Shift experiments using ts49-transformed lymphoid cells showed that at 39.5 degrees C they synthesize increased amounts of mu and kappa chain RNA and protein, and that they can be further induced to secrete IgM when treated with lipopolysaccharide.
D000011:Abelson murine leukemia virus / Q000235:genetics* / Q000276:immunology; D000818:Animals; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D005796:Genes; D005814:Genes, Viral; D006412:Hematopoietic Stem Cells / Q000276:immunology; D007075:Immunoglobulin M / Q000096:biosynthesis / Q000235:genetics*; D009052:Leukemia Virus, Murine / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D009154:Mutation; D011947:Receptors, Antigen, B-Cell / Q000235:genetics; D013696:Temperature
D007075:Immunoglobulin M; D011947:Receptors, Antigen, B-Cell