Measurement by bioluminescence technique of erythrocyte membrane Na+,K+-ATPase activity in hypertensive patients.
Erythrocyte membrane Na+,K+-ATPase activity was measured using a bioluminescence technique in 28 hypertensive patients (24 with essential hypertension, 2 with renovascular hypertension and 2 with hypertension secondary to primary hyperaldosteronism) and in 28 normotensive control subjects matched for age and sex. Erythrocyte Na+,K+-ATPase activity was significantly reduced in the patients with essential hypertension (130.9 +/- 11.4 vs. 186.6 +/- 19.5 nmol ATP/mg prot per h; mean values +/- SEM; p less than 0.05) and in the patients with secondary hypertension. A significant negative correlation was found between erythrocyte Na+,K+-ATPase and systolic blood pressure (r = -0.603; p less than 0.01), but not between Na+,K+-ATPase and plasma renin activity or plasma aldosterone levels. These data confirm the findings of a number of previous studies reporting reduced activity of erythrocyte Na+,K+-ATPase possibly related to the presence of a circulatory inhibitor of sodium pump. The method, based on ATP assay by bioluminescence, presents a high degree of specificity as well as simple, rapid execution.
D000328:Adult; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000201:enzymology*; D005260:Female; D006801:Humans; D006973:Hypertension / Q000201:enzymology*; D006978:Hypertension, Renovascular / Q000201:enzymology; D007546:Isoquinolines; D008163:Luminescent Measurements; D008297:Male; D008875:Middle Aged; D010042:Ouabain / Q000494:pharmacology; D000254:Sodium-Potassium-Exchanging ATPase / Q000097:blood* / Q000378:metabolism
D007546:Isoquinolines; D010042:Ouabain; C017475:lucifer yellow; D000254:Sodium-Potassium-Exchanging ATPase