title: Post-replication repair and recombination in uvrA umuC strains of Escherichia coli are enhanced by vanillin, an antimutagenic compound.
abstract: Effects of vanillin on UV killing of umuC mutant strains of E. coli were investigated in order to analyze the antimutagenic role of vanillin in mutagenesis. UV-irradiated uvrA umuC cells showed higher survival when plated on medium containing vanillin rather than medium without vanillin. This increased survival associated with exposure to vanillin was observed more clearly in uvrA umuC lexA(Ind-) and uvrA umuC recF strains. However, the effect was inhibited by additional recB recC mutations and completely blocked by an additional recA mutation. As far as tested the increased survival of UV-treated cells by vanillin was dependent on a capacity for genetic recombination. The effect of vanillin on recombination frequency between 2 plasmid DNA, pATH4 (Cmr Tcs) and pBMX7 (Apr Tcs), in a uvrA umuC background was investigated. A significantly higher frequency of plasmid recombination was observed when vanillin was present in the culture medium. These findings suggest that the antimutagenic effect of vanillin is the result of enhancement of a recA-dependent, error-free, pathway of post-replication repair.
mesh_terms: D001547:Benzaldehydes / Q000494:pharmacology*; D004260:DNA Repair / Q000187:drug effects*; D004261:DNA Replication; D004269:DNA, Bacterial / Q000187:drug effects / Q000235:genetics / Q000528:radiation effects; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics* / Q000528:radiation effects; D009154:Mutation; D010957:Plasmids; D011995:Recombination, Genetic / Q000187:drug effects*; D013014:SOS Response, Genetics / Q000187:drug effects; D014466:Ultraviolet Rays
chemical_list: D001547:Benzaldehydes; D004269:DNA, Bacterial; C100058:vanillin