Analysis of mutations occurring during replication of a SV40 shuttle vector in mammalian cells.
To analyse mutations that arise in mammalian cells we have used a SV40::plasmid shuttle vector containing a portion of the E. coli lacZ gene. We have found that following transfection into monkey Cos-7 cell mutations are not detected in the recovered plasmids at 24 h post transfection, but are found at 48 h post transfection, after the onset of DNA replication. Analysis of the mutant plasmids shows that in almost all cases the mutant phenotype is caused by a deletion or rearrangement of the lacZ gene in the shuttle vector.
D000818:Animals; D002460:Cell Line; D002872:Chromosome Deletion; D004261:DNA Replication; D005822:Genetic Vectors; D000882:Haplorhini; D007763:Lac Operon; D009154:Mutation; D010957:Plasmids; D011995:Recombination, Genetic; D013539:Simian virus 40 / Q000235:genetics*; D014162:Transfection; D014779:Virus Replication; D001616:beta-Galactosidase / Q000235:genetics
D001616:beta-Galactosidase