[New specific endonucleases PaeI and PaeII from Pseudomonas aeruginosa].
Specific endonuclease activities have been found it two Pseudomonas aeruginosa strains. Isolation and purification of enzymes and determining their specific activities have permitted one to find out that PaeI is an isoshizomer of SphI and digests the sequence 5'-GCATG C-3'. Another isolated enzyme PaeII is an isoshizomer of SmaI and cleaves DNA in a fragment 5'-CCC GGG-3'. The use of PaeI and PaeII enzymes in genetical engineering and their advantages are discussed.
D001483:Base Sequence; D004262:DNA Restriction Enzymes / Q000032:analysis* / Q000235:genetics; D015252:Deoxyribonucleases, Type II Site-Specific; D004591:Electrophoresis, Polyacrylamide Gel; D005818:Genetic Engineering; D011550:Pseudomonas aeruginosa / Q000201:enzymology* / Q000235:genetics
D004262:DNA Restriction Enzymes; C046184:endodeoxyribonuclease PaeI; C025651:CCCGGG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific
Novye spetsificheskie éndonukleazy PaeI i PaeII iz Pseudomonas aeruginosa.