title: [Properties of hepatitis A virus particles produced during infection in vitro].
abstract: Four types of virus-specific particles with different sedimentation coefficients and buoyant densities in CsCl were shown to be accumulated in hepatitis A virus (strain HAS-15) infected fetal rhesus monkey kidney cells (FRhK-4 line). Unlike the mature virions (155S, 1.34 g/cm3), cell-associated isosedimenting 92 S-particles (buoyant densities of 1.30 and 1.20 g/cm3) proved to be sensitive to lipase action. Particles of all four types were shown to contain similar sets of polypeptides, and, with the exception of "empty" 1.30 g/cm3-particles, appeared to be "full" under the immune electron microscopic examination. The viral RNA was unequivocally identified by the molecular hydridization test only in the mature virions.
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D006507:Hepatovirus / Q000032:analysis* / Q000276:immunology / Q000502:physiology; D008253:Macaca mulatta; D010455:Peptides / Q000032:analysis; D014764:Viral Proteins / Q000032:analysis; D014771:Virion / Q000032:analysis* / Q000276:immunology / Q000502:physiology; D014779:Virus Replication
chemical_list: D000956:Antigens, Viral; D010455:Peptides; D014764:Viral Proteins
vernacular_title: Svoĭstva chastits virusa gepatita A, formiruiushchikhsia pri vosproizvedenii infektsii in vitro.