title: Immunohistological evidence of lymphokine production and lymphocyte activation antigens in tuberculin reactions.
abstract: Evidence of lymphokine elaboration and lymphocyte activation was sought in tuberculin skin test reactions at 24 and 48, or 48 and 96 h in patients with active, culture-proven, pulmonary tuberculosis. Through the use of frozen sections, immunoperoxidase techniques and monoclonal antibodies, anti-interleukin 2 positive cells were found to constitute 0.4% to 0.6% of the dermal infiltrate, and keratinocyte Ia expression at 96 h was consistent with a marker for interferon-gamma production. Cells bearing the interleukin 2 receptor more than doubled in prevalence from 24 to 48 or 96 h but cells staining with Ta1, an antibody identifying activated lymphocytes, were 10% of the cells of the infiltrate at all three times. One-half of the cells of the infiltrate were OKM1-positive, presumably macrophages, perhaps reflecting the presence of active tuberculosis.
mesh_terms: D000954:Antigens, Surface / Q000032:analysis*; D004848:Epithelium / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D006801:Humans; D007124:Immunoenzyme Techniques; D007376:Interleukin-2 / Q000096:biosynthesis*; D008213:Lymphocyte Activation; D011948:Receptors, Antigen, T-Cell / Q000276:immunology; D012867:Skin / Q000276:immunology; D014374:Tuberculin Test; D014397:Tuberculosis, Pulmonary / Q000276:immunology*; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7
chemical_list: D000954:Antigens, Surface; D000949:Histocompatibility Antigens Class II; D007376:Interleukin-2; D011948:Receptors, Antigen, T-Cell; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7