title: Interactive roles of progesterone, prostaglandins, and collagenase in the ovulatory mechanism of the ewe.
abstract: Interrelationships between production of progesterone (P4), prostaglandin (PG) E2 and PGF2 alpha, and collagenase by periovulatory ovine follicles and their possible involvements in the ovulatory process were investigated. Follicles were isolated from ovaries at intervals (0 to 24 h) after the initiation of the preovulatory surge of luteinizing hormone (LH). Progesterone and PGs within follicles were determined by radioimmunoassay. Digestion of radioactive collagen during coincubation with tissue homogenates was used to assess the production of a bioactive follicular collagenase(s). Follicular accumulation of PGs and P4 increased at 12 and 16 h, respectively, after the onset of the surge of LH; PGE2 then decreased at 20 h. Collagenolytic activity of follicular tissue increased at 20 h and was maximal at 24 h (during the time of follicular rupture). An inhibitor of synthesis of P4 (isoxazol) or PGs (indomethacin) was injected into the follicular antrum at 8 h. Isoxazol did not prevent the initial rise in PGs, but inhibited synthesis of PGF2 alpha at 16 h and therafter. Isoxazol negated the decline in PGE2 and increase in collagenolysis. Indomethacin did not influence synthesis of P4; however, it suppressed collagenolytic activity of follicular tissue. Ovaries with treated follicles were left in situ and observed for an ovulation point at 30 h. Isoxazol or indomethacin was a potent inhibitor of ovulation. The blockade of ovulation by isoxazol was reversed by systemic administration of P4 or PGF2 alpha, but not by PGE2. Reversal of the blockade by indomethacin was accomplished with PGE2 or PGF2 alpha. Collagenolytic activity of follicular tissue was likewise restored by such treatments.(ABSTRACT TRUNCATED AT 250 WORDS)
mesh_terms: D000737:Androstenols / Q000494:pharmacology; D000818:Animals; D015237:Dinoprost; D015232:Dinoprostone; D005260:Female; D007213:Indomethacin / Q000494:pharmacology; D007700:Kinetics; D008297:Male; D003012:Microbial Collagenase / Q000096:biosynthesis*; D006080:Ovarian Follicle / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D010060:Ovulation / Q000187:drug effects; D011374:Progesterone / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D011458:Prostaglandins E / Q000096:biosynthesis*; D011460:Prostaglandins F / Q000096:biosynthesis*; D012756:Sheep; D014659:Vasectomy
chemical_list: D000737:Androstenols; D011458:Prostaglandins E; D011460:Prostaglandins F; C017039:azastene; D011374:Progesterone; D015237:Dinoprost; D003012:Microbial Collagenase; D015232:Dinoprostone; D007213:Indomethacin