In vivo competition of delta-crystallin gene expression by DNA fragments containing a GC box.
Expression of the chicken delta-crystallin gene 1 injected into the nuclei of mouse cells is lens specific. Coinjection of GC box-containing DNA fragments from delta-crystallin, simian virus 40 early, and herpes simplex virus type 1 tk promoters effectively suppressed delta-crystallin expression in the lens, but coinjection with DNA fragments not containing the GC box did not. This suppression was likely due to the competition of an Sp1-like transcription factor(s) and indicates involvement of the apparently ubiquitous factor(s) in the tissue-specific expression of the delta-crystallin gene.
D000818:Animals; D001483:Base Sequence; D003459:Crystallins / Q000235:genetics*; D005796:Genes; D005814:Genes, Viral; D011401:Promoter Regions, Genetic; D013539:Simian virus 40 / Q000235:genetics; D018139:Simplexvirus / Q000235:genetics
D003459:Crystallins