Physiological stress induced by sublethal concentrations of phenolic compounds in Notopterus notopterus: measurement of hydrolytic enzymes.
The studies on the effects of phenol (P), 2,4-dinitrophenol (DNP), pentachlorophenol (PCP) individually and in three combinations, viz., (PCP + DNP)/P (highly antagonistic), (DNP + P)/PCP (additive), and (P + DNP)/PCP (highly synergistic), at three sublethal levels (1/10, 1/15, and 1/20 of the 96-hr LC50) on the activity of hydrolytic enzymes (acid, alkaline, and glucose-6-phosphatases and 5-nucleotidase) in the brain and kidney of a fresh-water teleost, Notopterus notopterus revealed inhibition of enzyme activity after 15 and 30 days of exposure. Maximum inhibition (-89.32%) was observed in acid phosphatase activity in brain at 1/10 concentration of (P + DNP)/PCP combination after 30 days, but minimum (-3.64%) in activity of glucose-6-phosphatase in kidney at 1/20 of (PCP + DNP)/P combination after 15 days of exposure. However, biphasic effects of P, DNP, and (PCP + DNP)/P combination were observed in kidney, i.e., inhibition of enzymes activity at higher concentrations and stimulation at lower concentrations after both time intervals.
D015720:5'-Nucleotidase; D000135:Acid Phosphatase / Q000037:antagonists & inhibitors; D000469:Alkaline Phosphatase / Q000037:antagonists & inhibitors; D000818:Animals; D001921:Brain / Q000201:enzymology; D005399:Fishes / Q000378:metabolism*; D005952:Glucose-6-Phosphatase / Q000037:antagonists & inhibitors; D007668:Kidney / Q000201:enzymology; D009708:Nucleotidases / Q000037:antagonists & inhibitors*; D010085:Oxidative Phosphorylation / Q000187:drug effects; D010636:Phenols / Q000633:toxicity*; D010744:Phosphoric Monoester Hydrolases / Q000037:antagonists & inhibitors*
D010636:Phenols; D009708:Nucleotidases; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D010744:Phosphoric Monoester Hydrolases; D015720:5'-Nucleotidase; D005952:Glucose-6-Phosphatase