Immunogenic properties of mannose-containing ceramide tetrasaccharide from fresh-water bivalve, Hyriopsis schlegelii.
Antiserum against GlcNAc beta 1----2Man alpha 1----3Man beta 1----4Glc beta 1----1Cer (MlOse4Cer), a mannolipid isolated from spermatozoa of the fresh-water bivalve Hyriopsis schlegelii, was elicited in rabbits by repeated injection of a mixture of hapten-bovine serum albumin with Freund's adjuvant. The specificity of the affinity-purified antibody obtained from the serum was based on two forms of enzyme-immunodetection of its binding to structurally related glycolipids, either adsorbed to microtiter plates or chromatographed on thin-layer plates. The purified antibody exhibited a significant cross-reactivity with GlcNAc beta 1----2Man alpha 1----3(Xyl beta 1----2)Man beta 1----4Glc beta 1----1Cer, (MIXOse5Cer) containing a core structure closely related to MlOse4Cer, but almost unrelated to other glycolipids. Distribution of MlOse4Cer and MlXOse5Cer in various bivalve and snail glycolipid extracts were screened in thin-layer immunobinding assays by using this purified specific antibody. The presence of the glycolipid antigens was limited to certain taxonomic orders of shellfish species.
D000818:Animals; D000906:Antibodies / Q000302:isolation & purification; D000917:Antibody Formation; D000918:Antibody Specificity; D001666:Binding Sites, Antibody; D002855:Chromatography, Thin Layer; D005915:Globosides / Q000008:administration & dosage / Q000276:immunology* / Q000302:isolation & purification; D006028:Glycosphingolipids / Q000276:immunology*; D006241:Haptens / Q000008:administration & dosage / Q000276:immunology / Q000302:isolation & purification; D007124:Immunoenzyme Techniques; D008297:Male; D008974:Mollusca / Q000276:immunology*; D011817:Rabbits; D013094:Spermatozoa / Q000276:immunology
D000906:Antibodies; D005915:Globosides; D006028:Glycosphingolipids; D006241:Haptens; C057432:N-acetylglucosyl--2-mannosyl--3-mannosyl--4-glucosyl--1-ceramide