Detection of ABO blood group-active glycolipids extracted from red cell membrane and heat hematoma using TLC-immunostaining.
Glycolipids extracted from groups A, B, and O erythrocytes were developed on thin-layer plates; their ABO blood group antigenicities were detected by immunostaining method using avidin-biotin-complex (ABC). Among series of glycolipids of different flow rates, antigen-specific staining was observed in five bands from group A1 erythrocytes, four bands from group B, and two bands from group O. Monoclonal anti-A, -B, and -H antibodies specifically stained glycolipids from A1, B, and O erythrocytes, respectively. ABO blood grouping was possible from 5 g of epidural heat hematoma of a charred body by this method. ABC immunostaining on thin-layer chromatography is a useful and reliable method for ABO blood grouping in forensic practice.
D000017:ABO Blood-Group System / Q000235:genetics*; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D002056:Burns / Q000473:pathology*; D004910:Erythrocyte Membrane / Q000648:ultrastructure*; D006017:Glycolipids / Q000235:genetics*; D006407:Hematoma, Epidural, Cranial / Q000473:pathology*; D006801:Humans; D007124:Immunoenzyme Techniques
D000017:ABO Blood-Group System; D000911:Antibodies, Monoclonal; D006017:Glycolipids