[Lipopolysaccharide-induced hydrolysis of plasmalogenic phosphatidylethanolamine in human platelets].
The composition of human platelet major phospholipids-phosphatidylcholine (PC), phosphatidylinositol (PI), phosphatidylserine (PS), phosphatidic acid (PA), sphingomyelin (SM), plasmalogenic and diacyl species of phosphatidylethanolamine (PPE and APE, respectively) was quantitatively analyzed by high performance liquid chromatography. Incubation (10 min, 37 degrees C) of washed platelets with lipopolysaccharide B (LPS) of Salmonella typhimurium was found to produce (in the absence of aggregation) marked hydrolysis of PI (ca. 15%) and PPE (ca. 19%) containing the bulk of polyenic fatty acids. PC and APE were less degraded (8-9%), while the amounts of PS and SM were practically unchanged and the level of PA rose by 20%. Addition of thrombin to LPS-pretreated platelets resulted in their more rapid aggregation which was accompanied by a decreased and nearly equal hydrolysis of APE and PPE (7-8%) as compared with control platelets (10 and 12%, respectively). The extent to which PI was degraded (ca. 34%), by the action of thrombin was not affected by preliminary incubation with LPS. It is suggested that thrombin (as well as LPS) activating endogenous phospholipase(s) A2 can liberate from PPE not only arachidonic acid but also other essential polyenic fatty acids present in PPE in relatively high amounts. Besides, the agents studied may activate the intrinsic platelet system of rapid arachidonoyl transfer from diacyl PC and PE to PPE.
D001792:Blood Platelets / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D006868:Hydrolysis; D066298:In Vitro Techniques; D008070:Lipopolysaccharides / Q000494:pharmacology*; D010714:Phosphatidylethanolamines / Q000097:blood / Q000378:metabolism*; D010955:Plasmalogens / Q000097:blood / Q000378:metabolism*; D012486:Salmonella typhimurium
D008070:Lipopolysaccharides; D010714:Phosphatidylethanolamines; D010955:Plasmalogens
Indutsiruemyĭ lipopolisakharidom gidroliz plazmalogennogo fosfatidilétanolamina v trombotsitakh cheloveka.