Glycoproteins of human parainfluenza virus type 3: affinity purification, antigenic characterization and reconstitution into lipid vesicles.
Monoclonal antibodies to the envelope glycoproteins, HN and F, of human parainfluenza virus type 3 were coupled to a Sepharose 4B matrix and used for affinity purification of the viral glycoproteins. The purity of the glycoproteins was demonstrated by SDS-PAGE followed by fluorography or silver staining. The antigenicity of the glycoproteins was determined by immunization of rabbits; polyclonal rabbit antisera demonstrated inhibition of functional activities of the virus glycoproteins. The F glycoprotein, when reconstituted into lipid vesicles, showed distinct spike-like projections similar to those of intact virions.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology; D006023:Glycoproteins / Q000276:immunology / Q000302:isolation & purification*; D015707:HN Protein; D006389:Hemagglutinins, Viral / Q000276:immunology / Q000302:isolation & purification; D008081:Liposomes; D010224:Parainfluenza Virus 3, Human / Q000032:analysis* / Q000276:immunology; D011817:Rabbits; D018108:Respirovirus / Q000032:analysis*; D014759:Viral Envelope Proteins / Q000276:immunology / Q000302:isolation & purification*; D014760:Viral Fusion Proteins / Q000276:immunology / Q000302:isolation & purification*
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D006023:Glycoproteins; D015707:HN Protein; D006389:Hemagglutinins, Viral; D008081:Liposomes; D014759:Viral Envelope Proteins; D014760:Viral Fusion Proteins