A study of cyclic nucleotides as second messengers after interleukin 2 stimulation of human T lymphocytes.
Interleukin 2 (IL-2) was shown to induce a small but significant increase in the level of cGMP after 20 min stimulation and a subsequent fall after 1 h in activated T lymphocytes. No change in the level of cAMP was observed. Addition of the cyclic nucleotide analogues dbcAMP or dbcGMP did not stimulate DNA synthesis. On the contrary, IL-2-induced [3H]thymidine incorporation was inhibited by these drugs. Further, the phosphodiesterase inhibitor theophylline inhibited proliferation of activated T lymphocytes. Our results indicate that neither cAMP nor cGMP act as 'second messengers' for IL-2 but support the theory that cAMP is a negative regulator of cell proliferation.
D003994:Bucladesine / Q000494:pharmacology; D003995:Dibutyryl Cyclic GMP / Q000494:pharmacology; D006801:Humans; D007376:Interleukin-2 / Q000502:physiology*; D008213:Lymphocyte Activation / Q000187:drug effects; D009712:Nucleotides, Cyclic / Q000502:physiology*; D011971:Receptors, Immunologic / Q000502:physiology*; D015375:Receptors, Interleukin-2; D013601:T-Lymphocytes / Q000502:physiology*; D013806:Theophylline / Q000494:pharmacology
D007376:Interleukin-2; D009712:Nucleotides, Cyclic; D011971:Receptors, Immunologic; D015375:Receptors, Interleukin-2; D003995:Dibutyryl Cyclic GMP; D003994:Bucladesine; D013806:Theophylline