Partial purification and characterization of an aldohexose 1-P phosphatase from pig skeletal muscle.
An enzyme with a molecular weight of 54,000 which possesses phosphatase activity acting on glucose 1-P, galactose 1-P and mannose 1-P has been partially purified and characterized from pig skeletal muscle. The enzyme is free of phosphoglucomutase and galactokinase activities, and it possesses a neutral optimum pH. Pi acts as an inhibitor; glucose, galactose and mannose do not produce any effect. Divalent cations are required for activity, Mg2+ being the most effective activator. Micromolar levels of fluoride and millimolar levels of chloride act as inhibitors; however, vanadate does not produce any effect. The enzyme may have an important role when galactose accumulates in tissues; for example, in galactosemic patients and in young animals ingesting high-galactose diets.
D000818:Animals; D002413:Cations, Divalent; D002712:Chlorides / Q000494:pharmacology; D002845:Chromatography; D005459:Fluorides / Q000494:pharmacology; D005695:Galactosephosphates / Q000378:metabolism*; D005958:Glucosephosphates / Q000378:metabolism*; D006600:Hexosephosphates / Q000378:metabolism*; D008274:Magnesium / Q000494:pharmacology; D008360:Mannosephosphates / Q000378:metabolism*; D008970:Molecular Weight; D009132:Muscles / Q000201:enzymology*; D010710:Phosphates / Q000494:pharmacology; D010744:Phosphoric Monoester Hydrolases / Q000037:antagonists & inhibitors / Q000302:isolation & purification / Q000378:metabolism*; D013379:Substrate Specificity; D013552:Swine
D002413:Cations, Divalent; D002712:Chlorides; D005695:Galactosephosphates; D005958:Glucosephosphates; D006600:Hexosephosphates; D008360:Mannosephosphates; D010710:Phosphates; C029973:galactose-1-phosphate; C047217:mannose 1-phosphate; C031590:glucose-1-phosphate; D010744:Phosphoric Monoester Hydrolases; D008274:Magnesium; D005459:Fluorides