title: Expression of human papillomavirus types 6b and 16 L1 open reading frames in Escherichia coli: detection of a 56,000-dalton polypeptide containing genus-specific (common) antigens.
abstract: The human papillomavirus (HPV) genome contains two large open reading frames (ORFs), designated L1 and L2. To characterize the antigenic properties of the L1 ORF-encoded proteins, we cloned the L1 ORFs of HPV6b and HPV16 in plasmids, and these were expressed in Escherichia coli. First, the HPV6b DNA, representing 85.2% of the L1 ORF, was cloned in pUC19 and expressed in E. coli JM83 and RB791 as a 160,000-molecular-weight (160K) fusion protein with E. coli beta-galactosidase (6bL1/beta-gal). Second, the HPV16 DNA, representing 89.8% of the L1 ORF, was cloned in pKK233-2 and expressed as a 56K protein (16L1) in strain RB791. Both the 6bL1/beta-gal and 16L1 proteins cross-reacted with anti-bovine papillomavirus type 1 (BPV1) antibody raised against disrupted BPV1 particles. An antibody raised against the 6bL1/beta-gal fusion protein reacted with the 16L1 protein and also with native papillomavirus antigens in human genital condyloma and bovine fibropapilloma tissues, as determined by biotin-streptavidin staining. Furthermore, the anti-6bL1/beta-gal antibody recognized a 54K protein which seemed to be a major capsid protein of BPV1 and also a 56K protein of biopsies harboring HPV6 or HPV11. From these results we concluded that the papillomavirus L1 gene product contains genus-specific (common) antigens and that the HPV6 and HPV11 L1 genes specify the 56K capsid protein.
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000235:genetics*; D002417:Cattle; D003001:Cloning, Molecular; D003218:Condylomata Acuminata / Q000276:immunology / Q000382:microbiology; D004279:DNA, Viral / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D007818:Laryngeal Diseases / Q000276:immunology / Q000382:microbiology; D010212:Papilloma / Q000276:immunology / Q000382:microbiology; D027383:Papillomaviridae / Q000235:genetics* / Q000276:immunology; D010957:Plasmids; D002577:Uterine Cervical Diseases / Q000276:immunology / Q000382:microbiology; D014764:Viral Proteins / Q000235:genetics
chemical_list: D000956:Antigens, Viral; D004279:DNA, Viral; D014764:Viral Proteins