Functional expression of cloned cDNA encoding the alpha-subunit of adenylate cyclase-stimulating G-protein.
Cloned cDNA encoding the alpha-subunit of the adenylate cyclase-stimulating G-protein (Gs), carried by a simian virus 40 vector, has been introduced into the cyc- variant of S49 lymphoma cells by electroporation. In contrast to untransfected cys- cells, clones transformed with the cDNA exhibit an increase in intracellular cyclic AMP concentration in response to a beta-adrenergic agonist.
D000262:Adenylyl Cyclases / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular; D000242:Cyclic AMP / Q000378:metabolism; D004247:DNA / Q000378:metabolism*; D019204:GTP-Binding Proteins / Q000235:genetics*; D005796:Genes; D007545:Isoproterenol / Q000494:pharmacology; D008223:Lymphoma / Q000201:enzymology; D046911:Macromolecular Substances; D051379:Mice; D009693:Nucleic Acid Hybridization; D010957:Plasmids
D046911:Macromolecular Substances; D004247:DNA; D000242:Cyclic AMP; D019204:GTP-Binding Proteins; D000262:Adenylyl Cyclases; D007545:Isoproterenol