Synergistic binding of glucose and aluminium ATP to hexokinase from Saccharomyces cerevisiae.
The binding of glucose, AlATP and AlADP to the monomeric and dimeric forms of the native yeast hexokinase PII isoenzyme and to the proteolytically modified SII monomeric form was monitored at pH 6.7 by the concomitant quenching of intrinsic protein fluorescence. No fluorescence changes were observed when free enzyme was mixed with AlATP at concentrations up to 7500 microM. In the presence of saturating concentrations of glucose, the maximal quenching of fluorescence induced by AlATP was between 1.5 and 3.5% depending on species, and the average value of [L]0.5, the concentration of ligand at half-saturation, over all monomeric species was 0.9 +/- 0.4 microM. The presence of saturating concentrations of AlATP diminished [L]0.5 for glucose binding by between 260- and 670-fold for hexokinase PII and SII monomers, respectively (dependent on the ionic strength), and by almost 4000-fold for PII dimer. The data demonstrate extremely strong synergistic interactions in the binding of glucose and AlATP to yeast hexokinase, arising as a consequence of conformational changes in the free enzyme induced by glucose and in enzyme-glucose complex induced by AlATP. The synergistic interactions of glucose and AlATP are related to their kinetic synergism and to the ability of AlATP to act as a powerful inhibitor of the hexokinase reaction.
D000244:Adenosine Diphosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000535:Aluminum / Q000378:metabolism; D005453:Fluorescence; D005947:Glucose / Q000378:metabolism*; D006593:Hexokinase / Q000378:metabolism*; D007527:Isoenzymes / Q000378:metabolism; D009994:Osmolar Concentration; D011108:Polymers; D012441:Saccharomyces cerevisiae / Q000201:enzymology*
D007527:Isoenzymes; D011108:Polymers; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D000535:Aluminum; D006593:Hexokinase; D005947:Glucose