Phosphorylation of the alpha 1,2-linked mannosylsaccharide by N-acetylglucosamine-1-phosphotransferase from fibroblasts occurs at the terminal mannose.
UDP-GlcNAc: Lysosomal enzyme precursor N-acetylglucosamine-1-phosphotransferase activity from normal fibroblasts was measured using methyl 2-O-(alpha-D-mannopyranosyl) 6-deoxy-6-fluoro-alpha-D-mannopyranoside and methyl 2-O-(6-deoxy-6-fluoro-alpha-D-mannopyranosyl) alpha-D-mannopyranoside as acceptors. The results indicate that the phosphorylation in man alpha 1----2 man sequence occurs at the C-6 position of the terminal mannose residue.
D005347:Fibroblasts / Q000201:enzymology; D006801:Humans; D008358:Mannose / Q000378:metabolism*; D009844:Oligosaccharides / Q000378:metabolism*; D010766:Phosphorylation; D010770:Phosphotransferases / Q000494:pharmacology*; D017855:Transferases (Other Substituted Phosphate Groups)
D009844:Oligosaccharides; D010770:Phosphotransferases; D017855:Transferases (Other Substituted Phosphate Groups); C030012:UDP-N-acetylglucosamine-lysosomal-enzyme-N-acetylglucosaminephosphotransferase; D008358:Mannose