Chromosome abnormalities in chronic lymphocytic leukemia revealed by cytochalasin B and Epstein-Barr virus.
Peripheral blood lymphocytes from eight patients with chronic lymphocytic leukemia (CLL) were cultured with Epstein Barr virus (EBV) and cytochalasin B. All eight cytochalasin B cultures had analyzable metaphases whereas only six of the EBV cultures were successful. Furthermore, the number of abnormal metaphases and the mitotic indices were greater in the cytochalasin B cultures than in the EBV cultures. Trisomy 12, alone or in combination with other abnormalities, was the most frequent cytogenetic finding. Structural abnormalities of chromosomes #6 and #14 were also found. Cytochalasin B appears to be an effective mitogen for demonstrating abnormal metaphases in patients with CLL.
D000368:Aged; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D003571:Cytochalasin B; D005260:Female; D004854:Herpesvirus 4, Human; D006801:Humans; D007621:Karyotyping; D007945:Leukemia, Lymphoid / Q000235:genetics*; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000382:microbiology / Q000648:ultrastructure; D008297:Male; D008875:Middle Aged; D008934:Mitogens
D008934:Mitogens; D003571:Cytochalasin B