title: In vitro damage of basal lamina-associated anionic sites by hydroxyl radical.
abstract: We examined the effect of chemically generated hydroxyl radical on basal lamina-associated anionic sites. Cytochemical studies showed that hydroxyl radical produced a loss of cationic tracer-positive, anionic particles, and this effect was inhibited by a specific scavenger, thiourea. These data might suggest that anionic sites were degraded by hydroxyl radical which was derived, for example, from the activated leukocytes in close contact with the dermal-epidermal junction during acute inflammation resulting in the disturbance of the charge-selective permeability barrier.
mesh_terms: D000838:Anions / Q000378:metabolism*; D001485:Basement Membrane / Q000378:metabolism / Q000648:ultrastructure; D003872:Dermatitis / Q000139:chemically induced* / Q000473:pathology; D006801:Humans; D006878:Hydroxides / Q000494:pharmacology*; D017665:Hydroxyl Radical; D066298:In Vitro Techniques; D012867:Skin / Q000378:metabolism* / Q000473:pathology / Q000648:ultrastructure
chemical_list: D000838:Anions; D006878:Hydroxides; D017665:Hydroxyl Radical