Biochemical effects of gentamicin on rat kidney cortex. I. Analytical subfractionation of control tissue.
As a first step in studies of the molecular mechanism(s) underlying gentamicin toxicity, rat kidney cortex has been subfractionated using differential centrifugation. An analytical, rather than preparative approach was used. DNA was used as a marker for the nuclei, cytochrome oxidase for mitochondria, acid phosphatase for lysosomes, catalase for peroxisomes, NADPH-cytochrome c reductase for the endoplasmic reticulum, p-nitrophenyl-alpha-mannosidase (at pH 5.5) for the Golgi apparatus, AMPase for the plasma membrane in general, and alkaline phosphatase for the brush border, and lactate dehydrogenase for the cytosol. In addition, electron microscopy was performed on the subfractions obtained. The distributions of subcellular markers obtained here for the rat kidney cortex closely resemble the corresponding distributions reported for rat liver. This procedure can now be used to look for biochemical and/or toxic changes which might be reflected in an altered distribution pattern for marker enzymes.
D000118:Acetylglucosaminidase / Q000378:metabolism; D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D002374:Catalase / Q000378:metabolism; D002402:Cathepsin D / Q000378:metabolism; D002451:Cell Compartmentation; D003576:Electron Transport Complex IV / Q000378:metabolism; D004706:Endodeoxyribonucleases / Q000378:metabolism; D005839:Gentamicins / Q000633:toxicity; D007672:Kidney Cortex / Q000187:drug effects / Q000201:enzymology* / Q000648:ultrastructure; D008297:Male; D008361:Mannosidases / Q000378:metabolism; D009251:NADPH-Ferrihemoprotein Reductase / Q000378:metabolism; D009708:Nucleotidases / Q000378:metabolism; D051381:Rats; D013347:Subcellular Fractions / Q000201:enzymology; D043323:alpha-Mannosidase
D005839:Gentamicins; D002374:Catalase; D009251:NADPH-Ferrihemoprotein Reductase; D003576:Electron Transport Complex IV; D004706:Endodeoxyribonucleases; C019329:deoxyribonuclease II; D009708:Nucleotidases; C018775:adenosine monophosphatase; D000135:Acid Phosphatase; D008361:Mannosidases; D043323:alpha-Mannosidase; D000118:Acetylglucosaminidase; D002402:Cathepsin D