Assessment of anti-HLA antibodies in sera being tested for platelet reactivity by a platelet lymphocyte immunofluorescence test (PLIFT).
An indirect immunofluorescence test using a platelet mononuclear cell suspension is described. The test is a simple and sensitive method to assess the contamination by anti-HLA antibodies of sera being tested for platelet reactivity and eliminates the need to support two independent test systems such as lymphocytotoxicity and immunofluorescence testing. The test could be of value in routine platelet serology testing and platelet crossmatching.
D000937:Antigen-Antibody Reactions; D000961:Antilymphocyte Serum / Q000032:analysis*; D001792:Blood Platelets / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D006680:HLA Antigens / Q000276:immunology*; D006650:Histocompatibility Testing / Q000379:methods; D006801:Humans; D007106:Immune Sera / Q000032:analysis; D007518:Isoantibodies / Q000032:analysis*; D008214:Lymphocytes / Q000032:analysis*; D013535:Suspensions
D000961:Antilymphocyte Serum; D006680:HLA Antigens; D007106:Immune Sera; D007518:Isoantibodies; D013535:Suspensions