title: [Tumor necrosis factor].
abstract: Tumor necrosis factor (TNF) is cytokine derived from macrophage and shows much promise for application in cancer therapy because of its marked antitumor effects and its high specificity to tumors. Recently, the gene encoding human TNF was expressed in E. coli and the recombinant TNF was purified to homogeneity by ion exchange chromatography, affinity chromatography, and gel filtration. Clinical study of rH-TNF has been launched because human recombinant TNF can be produced on a large scale. In spite of notable antitumor effects, little is known concerning the mechanism of action of cytotoxic activity. In this article, the mechanism of action of rH-TNF against tumors in vitro and in vivo is reviewed.
mesh_terms: D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D003602:Cytotoxicity, Immunologic; D006023:Glycoproteins / Q000276:immunology / Q000627:therapeutic use*; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology; D008262:Macrophage Activation; D009369:Neoplasms / Q000276:immunology / Q000628:therapy*; D011956:Receptors, Cell Surface / Q000032:analysis; D018124:Receptors, Tumor Necrosis Factor; D014409:Tumor Necrosis Factor-alpha; D014410:Tumor Stem Cell Assay
chemical_list: D006023:Glycoproteins; D011956:Receptors, Cell Surface; D018124:Receptors, Tumor Necrosis Factor; D014409:Tumor Necrosis Factor-alpha