Causal role for an activated N-ras oncogene in the induction of tumorigenicity acquired by a human cell line.
ras oncogenes have been found in approximately 15% of the human tumors analyzed. However, a causal role for these genes in the tumorigenesis of human cells has yet to be shown. Tumorigenic late-passage PA-1 human teratocarcinoma cells (E-PA-1) contain an activated N-ras gene. In this report evidence is presented that nontumorigenic early passage revertant PA-1 cells (E-PA-1) contain only the germ-line protooncogene. Introduction by gene transfer of the activated L-PA-1 oncogene induces E-PA-1 cells to form tumors, suggesting that the activated N-ras oncogene has a causal role in the tumorigenesis of these cells.
D002448:Cell Adhesion; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis; D004262:DNA Restriction Enzymes / Q000378:metabolism; D015246:Deoxyribonuclease EcoRI; D006801:Humans; D009857:Oncogenes; D011519:Proto-Oncogenes; D013724:Teratoma / Q000235:genetics; D014162:Transfection
D004247:DNA; D004262:DNA Restriction Enzymes; D015246:Deoxyribonuclease EcoRI