title: 60Co gamma-rays induce predominantly C/G to G/C transversions in double-stranded M13 DNA.
abstract: Upon irradiation with gamma rays of an oxygenated aqueous solution of double-stranded M13 DNA, a very specific mutation spectrum was found with respect to both the type and the positions in the DNA sequence. Of the 23 mutations, which were sequenced, 16 represent a C/G to G/C transversion. A C/G to T/A transition was found once and a G/C to T/A transversion twice. The remaining 4 mutations are frameshifts, 2 are identical and formed by the insertion of a G/C basepair; the other 2 mutations are due to a duplication of 10 basepairs situated at different positions but with a remarkable homology in base sequence. Fourteen mutations, including the 2 duplications are found in the neighbourhood of a TGCT/ACGA sequence.
mesh_terms: D001483:Base Sequence / Q000528:radiation effects; D003037:Cobalt Radioisotopes; D003090:Coliphages / Q000235:genetics* / Q000528:radiation effects; D004247:DNA / Q000528:radiation effects*; D004249:DNA Damage; D004269:DNA, Bacterial / Q000187:drug effects; D004926:Escherichia coli / Q000235:genetics / Q000528:radiation effects; D005720:Gamma Rays; D007763:Lac Operon / Q000528:radiation effects; D008969:Molecular Sequence Data; D009154:Mutation; D011839:Radiation, Ionizing
chemical_list: D003037:Cobalt Radioisotopes; D004269:DNA, Bacterial; D004247:DNA