Enzyme markers of collagen synthesis in carbon tetrachloride-induced fibrosis and during colchicine modification of CCl4-induced liver injury.
Serum galactosylhydroxylysyl glucosyltransferase (S-Glu-Gal-Hyl-Tase), liver galactosylhydroxylysyl glucosyltransferase (L-Glu-Gal-Hyl-Tase), liver hydroxylysyl galactosyltransferase (L-Gal-Hyl-Tase), and liver prolyl hydroxylase (L-PH) activities were measured in rats during the development of CCl4-induced cirrhosis (0.2 ml of 33% CCl4 in light mineral oil two times weekly for 10 weeks followed by 6 weeks of no treatment). Serum and liver markers of collagen synthesis increased in a time-dependent manner reaching maximum activity at 6 weeks (S-Glu-Gal-Hyl-Tase, two times; L-PH, two times). These enzyme levels returned to normal during the 4-week recovery period. In a separate 4-week experiment, colchicine (10 micrograms/rat/day) was administered with CCl4. Colchicine prevented the increase in S-Glu-Gal-Hyl-Tase, L-Glu-Gal-Hyl-Tase, and L-Gal-Hyl-Tase induced by CCl4 and resulted in a smaller increase in L-PH. These results demonstrate that S-Glu-Gal-Hyl-Tase elevation occurs following CCl4 because of increased liver collagen synthetic activity and the hepatocellular injury produced by CCl4.
D000818:Animals; D002252:Carbon Tetrachloride Poisoning / Q000378:metabolism*; D003078:Colchicine / Q000494:pharmacology*; D003094:Collagen / Q000096:biosynthesis*; D005700:Galactosyltransferases / Q000378:metabolism*; D005964:Glucosyltransferases / Q000097:blood / Q000378:metabolism*; D008099:Liver / Q000201:enzymology; D008297:Male; D011393:Procollagen-Proline Dioxygenase / Q000378:metabolism*; D051381:Rats; D013997:Time Factors
D003094:Collagen; D011393:Procollagen-Proline Dioxygenase; D005700:Galactosyltransferases; D005964:Glucosyltransferases; C023236:UDPgalactose-collagen galactosyltransferase; C023247:UDP glucose-collagen glucosyltransferase; D003078:Colchicine