Determination of adenosine ribo- and deoxyribonucleotides as their 1-N6-etheno derivatives by reversed-phase ion-pair high-performance liquid chromatography.
The chromatographic resolution of fluorescent 1-N6-etheno derivatives of adenine and adenosine ribo- and deoxyribonucleotides on reversed-phase columns has been optimised by a systematic study of the effect of ion-pair concentration, pH, eluent molarity and methanol concentration. Using tetrabutylammonium hydrogen sulphate as the ion-pair separation of up to eight derivatives could be achieved in ca. 10 min. Conditions of derivatisation with chloroacetaldehyde have been investigated in order to reduce hydrolysis of the nucleotides.
D000227:Adenine Nucleotides / Q000032:analysis*; D000241:Adenosine / Q000031:analogs & derivatives* / Q000032:analysis; D002021:Buffers; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D005456:Fluorescent Dyes; D006868:Hydrolysis; D010084:Oxidation-Reduction; D010504:Periodic Acid; D013056:Spectrophotometry, Ultraviolet
D000227:Adenine Nucleotides; D002021:Buffers; D005456:Fluorescent Dyes; D010504:Periodic Acid; C007640:1,N(6)-ethenoadenosine; D000241:Adenosine