Oxidation of reduced cytochrome c by hydrogen peroxide. Implications for superoxide assays.
Hydrogen peroxide, formed directly or as a product of superoxide dismutation, can oxidize ferrocytochrome c at rates comparable to those at which ferricytochrome c is reduced by superoxide. This reoxidation can significantly affect estimates of rates and amounts of superoxide production using absorbance changes for cytochrome c at 550 nm as the assay. The oxidation can be inhibited by catalase.
D000818:Animals; D003574:Cytochrome c Group / Q000378:metabolism*; D006736:Horses; D006861:Hydrogen Peroxide / Q000378:metabolism*; D007700:Kinetics; D009206:Myocardium / Q000378:metabolism; D010084:Oxidation-Reduction; D013053:Spectrophotometry / Q000379:methods; D013481:Superoxides / Q000032:analysis*; D014527:Uric Acid / Q000032:analysis
D003574:Cytochrome c Group; D013481:Superoxides; D014527:Uric Acid; D006861:Hydrogen Peroxide