title: Host-dependent temperature-sensitive growth of HVJ (Sendai virus) wild-type in rat glioma C 6 cells.
abstract: At non-permissive temperature viral specific RNA synthesis was not restricted in rat glioma (C 6) cells infected with HVJ (Sendai virus) wild-type. However, as has previously been shown (J Gen Virol [1984] 65: 639-643), the synthesis of M protein was reduced at non-permissive temperature, in contrast to the L, P, HN, Fo and NP proteins which were synthesized in comparable amounts at permissive and non-permissive temperatures. In this report we show additionally that viral nucleocapsids (NC), which consist of L, P and NP proteins, were formed within the infected cells at both temperatures. Hemagglutinin and neuraminidase activities were also detected in samples incubated at non-permissive temperature. By membrane immunofluorescence and cell-surface immunoprecipitation it was shown that migration of HN and Fo proteins to the cell surface occurred normally at non-permissive temperature. Additionally, the L, P and NP proteins, which were associated with the plasma membrane isolated from the infected cells maintained at permissive temperature, were absent from the membrane of cells incubated at non-permissive temperature. These results suggest that NC and glycoproteins synthesized at non-permissive temperature could not assemble effectively at the plasma membrane because of a lack of M protein. Thus, the host-dependent ts lesion of HVJ in C 6 cells was considered to be mainly in M protein synthesis.
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000096:biosynthesis; D002460:Cell Line; D002642:Chick Embryo; D005347:Fibroblasts / Q000382:microbiology; D005910:Glioma / Q000382:microbiology / Q000473:pathology*; D006023:Glycoproteins / Q000096:biosynthesis / Q000276:immunology; D008253:Macaca mulatta; D010222:Parainfluenza Virus 1, Human / Q000254:growth & development* / Q000276:immunology; D012367:RNA, Viral / Q000096:biosynthesis; D051381:Rats; D013696:Temperature; D014763:Viral Matrix Proteins; D014764:Viral Proteins / Q000096:biosynthesis; D014775:Virus Activation; D014776:Virus Cultivation; D014779:Virus Replication
chemical_list: D000956:Antigens, Viral; D006023:Glycoproteins; D012367:RNA, Viral; D014763:Viral Matrix Proteins; D014764:Viral Proteins