Immunoprecipitation of a male-specific polypeptide after in vitro translation of testicular poly(A)+ RNA.
Poly(A)+ RNAs from male and female gonads of 20-day-old rats were translated in vitro using rabbit reticulocyte lysates. The resulting polypeptides were incubated with specific anti-male (anti-H-Y) antisera raised in female rats by intrasplenic immunization with syngeneic male skin. Using a second antibody, a male-specific polypeptide of molecular weight approximately 18,000 was immunoprecipitated. This male-specific polypeptide was not cotranslationally modified in vitro and appeared to be identical to serological H-Y antigen.
D000818:Animals; D002474:Cell-Free System; D006182:H-Y Antigen / Q000235:genetics*; D007158:Immunologic Techniques; D008297:Male; D008970:Molecular Weight; D011499:Protein Processing, Post-Translational; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D013737:Testis / Q000502:physiology*
D006182:H-Y Antigen; D012333:RNA, Messenger