Crystal-induced inflammation in the rat subcutaneous air-pouch.
Monosodium urate (MSU) and calcium pyrophosphate dihydrate (CPPD) crystals initiated acute inflammatory reactions characterized by increased plasma extravasation and polymorphonuclear leukocyte (PMNL) accumulation in the rat subcutaneous air-pouch. Pretreatment of rats with colchicine (1 mg kg-1, s.c.) inhibited PMNL accumulation induced by either crystal type but had a greater inhibitory effect on MSU-induced plasma extravasation compared with that induced by CPPD crystals. Colchicine (1 mg kg-1, s.c.) did not reduce histamine-induced plasma extravasation in the air-pouch. The lipoxygenase product of arachidonic acid metabolism, leukotriene B4 (LTB4), was detected in MSU-induced exudates but not in CPPD-induced exudates. Pretreatment of rats with colchicine (1 mg kg-1, s.c.) inhibited LTB4 production in MSU-induced exudates.
D000818:Animals; D000893:Anti-Inflammatory Agents; D002131:Calcium Pyrophosphate / Q000037:antagonists & inhibitors / Q000633:toxicity*; D003078:Colchicine / Q000494:pharmacology*; D011756:Diphosphates / Q000633:toxicity*; D004195:Disease Models, Animal; D007249:Inflammation / Q000139:chemically induced* / Q000378:metabolism / Q000517:prevention & control; D007975:Leukotriene B4 / Q000378:metabolism; D008297:Male; D009504:Neutrophils / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains; D014527:Uric Acid / Q000037:antagonists & inhibitors / Q000633:toxicity*
D000893:Anti-Inflammatory Agents; D011756:Diphosphates; D007975:Leukotriene B4; D014527:Uric Acid; D003078:Colchicine; D002131:Calcium Pyrophosphate