[Transformation of bone marrow cells in rodents by recombinant plasmid pBRSV].
Bone marrow cells (mouse strain CBA/Ca and Syrian hamster cells) were transformed with pBRSV DNA containing T-antigen of the SV40 virus. The SV40 T-antigen in transformed cell was detected in 0.5% cases by immunofluorescence with specific antibodies. Extrachromosomal localization of recombinant DNA was shown by means of retransformation of E. coli cells with cytoplasmic spleen DNA from mice previously injected intravenously the transformed bone marrow cells.
D000818:Animals; D000957:Antigens, Viral, Tumor / Q000235:genetics; D001853:Bone Marrow / Q000187:drug effects; D001854:Bone Marrow Cells; D002472:Cell Transformation, Viral / Q000187:drug effects*; D002478:Cells, Cultured; D006224:Cricetinae; D004274:DNA, Recombinant / Q000494:pharmacology*; D004279:DNA, Viral / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D008647:Mesocricetus; D051379:Mice; D008808:Mice, Inbred CBA; D010957:Plasmids; D013539:Simian virus 40 / Q000235:genetics
D000957:Antigens, Viral, Tumor; D004274:DNA, Recombinant; D004279:DNA, Viral
Transformatiia kletok kostnogo mozga gryzunov rekombinantnoĭ plazmidoĭ pBRSV.