Metabolism of glycerate 2,3-P2--XII. Characterization of the 2,3-bisphosphoglycerate synthase-phosphatase and of the hybrid phosphoglycerate mutase/2,3-bisphosphoglycerate synthase-phosphatase from pig brain.
2,3-Bisphosphoglycerate synthase-phosphatase and the hybrid phosphoglycerate mutase/2,3-bisphosphoglycerate synthase-phosphatase have been partially purified from pig brain. Their 2,3-bisphosphoglycerate synthase, 2,3-bisphosphoglycerate phosphatase and phosphoglycerate mutase activities are concurrently lost upon heating and treatment with reagents specific for histidyl, arginyl and lysyl residues. The two enzymes differ in their thermal stability and sensitivity to tetrathionate. Substrates and cofactors protect against inactivation, the protective effects varying with the modifying reagent. The synthase activity of both enzymes shows a nonhyperbolic pattern which fits to a second degree polynomial. The Km, Ki and optimum pH values are similar to those of the 2,3-bisphosphoglycerate synthase-phosphatase from erythrocytes and the hybrid enzyme from skeletal muscle. The synthase activity is inhibited by inorganic phosphate and it is stimulated by glycolyate 2-P.
D019794:2,3-Diphosphoglycerate; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001731:Bisphosphoglycerate Mutase / Q000235:genetics / Q000302:isolation & purification*; D001921:Brain / Q000201:enzymology*; D002845:Chromatography / Q000379:methods; D004163:Diphosphoglyceric Acids / Q000378:metabolism*; D004795:Enzyme Stability; D006824:Hybridization, Genetic; D007527:Isoenzymes / Q000235:genetics / Q000302:isolation & purification*; D007700:Kinetics; D008970:Molecular Weight; D010744:Phosphoric Monoester Hydrolases / Q000302:isolation & purification*; D010770:Phosphotransferases / Q000302:isolation & purification*; D011487:Protein Conformation; D013552:Swine
D000596:Amino Acids; D004163:Diphosphoglyceric Acids; D007527:Isoenzymes; C019794:bisphosphoglycerate phosphatase; D019794:2,3-Diphosphoglycerate; D010770:Phosphotransferases; D010744:Phosphoric Monoester Hydrolases; D001731:Bisphosphoglycerate Mutase