Direct extraction and assay of bone tissue collagenase and its relation to parathyroid-hormone-induced bone resorption.
A method has been developed for the quantitative extraction of collagenase from as little as one 19-day-fetal-mouse calvarium. About 20-40 munits of collagenase are extracted per mg of tissue, all in a latent form that, after proper activation, shows the typical properties of mammalian collagenase. Culturing the calvaria for 2 days with parathyroid hormone (PTH) increases their procollagenase content up to 3-fold and induces bone resorption. Both PTH effects are prevented by cycloheximide, but not by indomethacin. Calcitonin inhibits resorption without affecting the PTH-induced procollagenase synthesis. The role of this synthesis is discussed in relation to the mechanisms of bone resorption.
D000818:Animals; D001862:Bone Resorption / Q000187:drug effects*; D001842:Bone and Bones / Q000187:drug effects / Q000201:enzymology*; D002116:Calcitonin / Q000494:pharmacology; D003513:Cycloheximide / Q000494:pharmacology; D007213:Indomethacin / Q000494:pharmacology; D051379:Mice; D003012:Microbial Collagenase / Q000037:antagonists & inhibitors / Q000302:isolation & purification* / Q000378:metabolism; D009924:Organ Culture Techniques
D002116:Calcitonin; D003513:Cycloheximide; D003012:Microbial Collagenase; D007213:Indomethacin