Effects of urinastatin on cardiac energy metabolism in acute hemorrhage in rats.
Urinastatin, one of the trypsin inhibitors extracted from the human urine, has been reported to be useful in treating hypovolemic shock. We studied the effects of urinastatin on cardiac energy metabolism in acute hemorrhage in rats. The hemorrhage was produced by withdrawing blood (60 ml) from the abdominal aorta, and an equivalent volume of saline and 25,000 units/kg of urinastatin were injected over 5 min. The heart was then frozen quickly with liquid nitrogen, and high energy phosphates, lactate, pyruvate and glycogen contents were measured enzymatically. There was no significant difference in either ATP, lactate or pyruvate contents of the heart between the urinastatin-treated and the control groups. However, energy charge ([ATP + 0.5 X ADP]/[ATP + ADP + AMP]) and glycogen levels in the urinastatin-treated group were significantly higher than those in the control group. These findings suggest that urinastatin might produce a metabolic improvement in mitochondrial function and suppress the anaerobic glycogenolysis induced by circulatory deficiency in an acute hemorrhagic state.
D000227:Adenine Nucleotides / Q000378:metabolism; D000818:Animals; D004734:Energy Metabolism / Q000187:drug effects*; D006023:Glycoproteins / Q000494:pharmacology*; D006321:Heart / Q000187:drug effects*; D006470:Hemorrhage / Q000378:metabolism*; D008297:Male; D009206:Myocardium / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D000227:Adenine Nucleotides; D006023:Glycoproteins; C028665:urinastatin