Identification and characterization of a high density lipoprotein-binding protein in cell membranes by ligand blotting.
Cholesterol efflux from cultured cells can be mediated through binding of high density lipoprotein (HDL) to a cell-surface site which shows many characteristics of a biological receptor. To determine whether a specific protein forms a component of this site, cell membrane proteins were analyzed by ligand blotting using 125I-HDL3. Results demonstrated that membranes from a number of cell types possess a protein with an apparent molecular mass of 110 kDa that binds HDL and apoA-I and apoA-II proteoliposomes, but not low density lipoprotein, acetylated low density lipoprotein, or apoE proteoliposomes. The binding activity of this protein was increased by loading cells with cholesterol and was abolished by trypsin treatment of intact cell monolayers. These results suggest that HDL binds with specificity to a cell-surface protein which is regulated by intracellular cholesterol levels. Since HDL binding to intact cell monolayers shows the same characteristics, the 110-kDa binding protein may represent the proposed HDL receptor that functions to facilitate transport of cholesterol from cells to HDL particles.
D000818:Animals; D002352:Carrier Proteins; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D006801:Humans; D007700:Kinetics; D008075:Lipoproteins, HDL / Q000378:metabolism*; D008081:Liposomes; D008970:Molecular Weight; D011510:Proteolipids; D016601:RNA-Binding Proteins; D011956:Receptors, Cell Surface / Q000302:isolation & purification / Q000378:metabolism*; D018110:Receptors, Lipoprotein; D014018:Tissue Distribution
D002352:Carrier Proteins; D008075:Lipoproteins, HDL; D008081:Liposomes; D011510:Proteolipids; D016601:RNA-Binding Proteins; D011956:Receptors, Cell Surface; D018110:Receptors, Lipoprotein; C031616:high density lipoprotein receptors; C020485:proteoliposomes; C075197:high density lipoprotein binding protein