Specific binding of leukotriene C4 to endothelial cell membranes.
Vascular endothelium is a target for leukotriene C4 (LTC4) as demonstrated by previous in vivo and culture experiments. Binding assays were carried out at 0 degrees C on membrane fraction obtained from bovine aortic endothelial cells in culture. Specific binding sites (Kd = 49.9 +/- 6.3 nmol X 1(-1), N = 1.2 X 10(6) sites per cell) for LTC4 were demonstrated in this preparation. Competition studies showed that LTB4, LTD4 and LTE4 did not displace LTC4 from its binding sites. FPL 55712, a sulfidopeptide antagonist, was seen to be a weak competitor and reduced glutathione exhibited a significant affinity for the binding site. The possible receptor role of this site is discussed.
D000818:Animals; D001011:Aorta / Q000166:cytology; D001667:Binding, Competitive; D002417:Cattle; D002462:Cell Membrane / Q000378:metabolism*; D002478:Cells, Cultured; D002867:Chromones / Q000378:metabolism; D004727:Endothelium / Q000378:metabolism*; D007975:Leukotriene B4 / Q000378:metabolism; D017999:Leukotriene E4; D013189:SRS-A / Q000031:analogs & derivatives / Q000378:metabolism*
D002867:Chromones; D013189:SRS-A; D007975:Leukotriene B4; D017999:Leukotriene E4; C006312:FPL 55712