[Structural organization of chromatin as a factor determining the rate of its endonucleolysis in irradiated and nonirradiated thymocytes].
A study was made of chromatin endonucleolysis in hypotonic thymocytes incubated in digestive buffers containing different concentrations of potassium, magnesium, calcium, and mercaptoethanol. Inhibition of endonucleolysis by univalent cation during the first 20 min of incubation was followed by intensive chromatin degradation. A decrease in free potassium content retarded chromatin degradation and enhanced the inhibiting effect of the univalent cations. The regularities of changes in the rate of chromatin endonucleolysis in different digestive buffers were similar with both exposed and intact thymocytes.
D000818:Animals; D002021:Buffers; D002478:Cells, Cultured; D002843:Chromatin / Q000201:enzymology / Q000528:radiation effects* / Q000648:ultrastructure; D004720:Endonucleases / Q000528:radiation effects*; D005720:Gamma Rays; D007038:Hypotonic Solutions; D008297:Male; D051381:Rats; D013950:Thymus Gland / Q000201:enzymology / Q000528:radiation effects* / Q000648:ultrastructure
D002021:Buffers; D002843:Chromatin; D007038:Hypotonic Solutions; D004720:Endonucleases
Strukturnaia organizatsiia khromatina kak faktor, opredeliaiushchiĭ skorost' ego éndonukleoliza v obluchennykh i neobluchennykh timotsitakh.