Effects of naftidrofuryl on adrenergic nerves, endothelium and smooth muscle in isolated canine blood vessels.
Experiments were designed to determine the effects of the vasoactive drug naftidrofuryl on vascular smooth muscle, endothelial cells and adrenergic nerves in isolated canine blood vessels. Naftidrofuryl inhibited contractions of basilar arteries (in a decreasing order of potency), evoked by 5-hydroxytryptamine greater than KCl = anoxia (in rings with endothelium) greater than prostaglandin F2 alpha = uridine-5'-triphosphate. Naftidrofuryl antagonized competitively the contractions evoked by 5-hydroxytryptamine in the femoral artery and the saphenous vein. Naftidrofuryl caused the release of an endothelium-derived relaxing factor(s) from the endothelium of femoral arteries. The compound depressed contractions of saphenous veins evoked by electrical stimulation of the adrenergic nerve endings, but not those caused by the indirect sympathomimetic amine tyramine or exogenous norepinephrine. In saphenous veins incubated previously with [3H]norepinephrine, the drug inhibited the contractions and the release of transmitter evoked by electrical stimulation. Thus, naftidrofuryl acts at different levels in the blood vessel wall to cause: release of endothelium-derived relaxing factor(s); inhibition of S2-serotonergic receptors on vascular smooth muscle; prejunctional inhibition of adrenergic neurotransmission; and nonselective inhibition of the contractile process in vascular smooth muscle, which is particularly pronounced in cerebral arteries.
D000320:Adrenergic Fibers / Q000187:drug effects*; D000818:Animals; D001681:Biological Assay; D003042:Cocaine / Q000494:pharmacology; D015237:Dinoprost; D004285:Dogs; D004558:Electric Stimulation; D004727:Endothelium / Q000187:drug effects; D005260:Female; D005263:Femoral Artery / Q000187:drug effects; D005663:Furans / Q000494:pharmacology*; D000860:Hypoxia / Q000503:physiopathology; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000187:drug effects*; D009257:Nafronyl / Q000494:pharmacology*; D009638:Norepinephrine / Q000494:pharmacology; D011460:Prostaglandins F / Q000494:pharmacology; D012501:Saphenous Vein / Q000187:drug effects; D012701:Serotonin / Q000494:pharmacology; D009435:Synaptic Transmission / Q000187:drug effects; D014661:Vasoconstriction / Q000187:drug effects
D005663:Furans; D011460:Prostaglandins F; D012701:Serotonin; D009257:Nafronyl; D015237:Dinoprost; D003042:Cocaine; D009638:Norepinephrine