title: Sensitivity of polA mutants of Escherichia coli K-12 to ozone and radiations.
abstract: Different polA mutants of Escherichia coli K-12 were exposed to ozone, X-rays and UV radiation, in order to compare the role of the various enzymatic activities of DNA polymerase I in the repair of damage caused by these agents. As was the case with radiations, the polymerase-deficient mutants were the most sensitive to ozone, followed by the 5'-3' exonuclease-deficient mutants. The 3'-5' exonuclease activity of pol I appeared to play a minor role in the repair of damage induced by all these agents.
mesh_terms: D004256:DNA Polymerase I / Q000235:genetics* / Q000528:radiation effects; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics* / Q000528:radiation effects; D009154:Mutation; D010126:Ozone / Q000633:toxicity*; D013045:Species Specificity; D014466:Ultraviolet Rays / Q000009:adverse effects*; D014965:X-Rays / Q000009:adverse effects
chemical_list: D010126:Ozone; D004256:DNA Polymerase I