title: Restriction endonuclease patterns of bovine herpesvirus type 1 isolated from bovine mammary glands.
abstract: Restriction endonuclease analysis was used, in conjunction with viral neutralization and growth-curve experiments, to compare a bovine herpesvirus type 1 (BHV-1) isolate, originally obtained from bovine mammary gland lesions, with a standard BHV-1 strain, infectious bovine rhinotracheitis virus. Although differences were not detected by viral neutralization or growth-curve experiments, restriction fragment patterns generated by Bam HI, Eco RI, Hind III, and Hpa I, revealed definite differences between the isolate and the prototype strain. Additionally, Eco RI, Hind III, and Hpa I patterns revealed that the mammary gland isolate had DNA-fragment patterns characteristic of infectious pustular vulvovaginitis strain of BHV-1, type 2b. Seemingly, type-2b isolates, similar to types 1 and 2a, may be capable of causing divergent types of infection of variable severity in cattle.
mesh_terms: D000818:Animals; D001426:Bacterial Proteins; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000032:analysis*; D015245:Deoxyribonuclease BamHI; D015246:Deoxyribonuclease EcoRI; D015247:Deoxyribonuclease HindIII; D015252:Deoxyribonucleases, Type II Site-Specific; D005260:Female; D006566:Herpesviridae Infections / Q000382:microbiology / Q000662:veterinary*; D007242:Herpesvirus 1, Bovine / Q000235:genetics* / Q000254:growth & development; D008321:Mammary Glands, Animal / Q000382:microbiology
chemical_list: D001426:Bacterial Proteins; D004279:DNA, Viral; D004262:DNA Restriction Enzymes; D015245:Deoxyribonuclease BamHI; D015246:Deoxyribonuclease EcoRI; D015247:Deoxyribonuclease HindIII; C024406:endodeoxyribonuclease HpaI; C024404:BglII endonuclease; D015252:Deoxyribonucleases, Type II Site-Specific