Detection of infectious pancreatic necrosis virus using an inhibition enzyme-linked immunosorbent assay.
An inhibition enzyme-linked immunosorbent assay was used to detect infectious pancreatic necrosis (IPN) virus. In this assay the presence of virus was determined by measuring the decrease in titer of a known antiserum after incubation with a sample suspected to contain virus. The titer of the antiserum was measured with an indirect enzyme-linked assay. Compared to the double antibody sandwich method this assay required fewer reagents (only one anti-IPN serum was required). This assay was also sensitive enough to detect virus at levels of 1 X 10(2) TCID 50/ml. of purified virus and was able to detect virus in samples obtained in the field.
D000818:Animals; D002432:Cecum / Q000382:microbiology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005393:Fish Diseases / Q000175:diagnosis / Q000382:microbiology*; D007106:Immune Sera; D007668:Kidney / Q000382:microbiology; D010182:Pancreatic Diseases / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D012087:Reoviridae / Q000302:isolation & purification*; D012088:Reoviridae Infections / Q000175:diagnosis / Q000662:veterinary*; D013154:Spleen / Q000382:microbiology; D014337:Trout
D007106:Immune Sera