Purification, crystallisation and preliminary X-ray diffraction characterisation of methanol dehydrogenase from Methylosinus trichosporium OB3b.
Methanol dehydrogenase was purified from the obligate methanotroph, Methylosinus trichosporium OB3b, in two steps from disrupted biomass by aqueous two-phase partition and ion-exchange chromatography. Copartitioning of a cytochrome c was dependent upon the pH at which aqueous partition was carried out. The native enzyme has a Mr of 120,000, as determined by gel filtration chromatography, and consists of two identical subunits. The purified enzyme contained four electrophoretically distinct isoenzymes, with pI values of 6.3, 6.58, 6.63 and 6.88. The native enzyme has been crystallised in a form suitable for high-resolution X-ray crystallographic studies. The crystals diffract to better than 0.19 nm spacing and are relatively stable to irradiation with X-rays. The space group is P6(1)22 (or P6(5)22) with cell dimensions a = b = 10.21 nm, c = 29.32 nm and the crystal probably contains a single monomer in the asymmetric unit.
D000429:Alcohol Oxidoreductases / Q000302:isolation & purification*; D002850:Chromatography, Gel; D003460:Crystallization; D003574:Cytochrome c Group / Q000378:metabolism; D019605:Euryarchaeota / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D007526:Isoelectric Point; D007527:Isoenzymes / Q000302:isolation & purification; D046911:Macromolecular Substances; D008970:Molecular Weight; D014961:X-Ray Diffraction
D003574:Cytochrome c Group; D007527:Isoenzymes; D046911:Macromolecular Substances; D000429:Alcohol Oxidoreductases; C019641:alcohol dehydrogenase (acceptor)