Disseminated intravascular coagulation (DIC) induced by liquoid (polyanetholsulfonate) in the rat. V. Effects on circulating fibronectin.
Levels of plasma fibronectin (Fn) were 63% lower than normal 15 min after the intravenous injection of liquoid (P less than 0.01); 3 h later they were still low but rebounded to 35% above normal (P less than 0.01) by 24 h. Concurrently microthrombi containing fibrinogen, Fn and Factor VIII related-antigens (VIII:Ag) were detected in the kidneys and lungs by immunohistopathological studies. Ultrastructurally, thrombi were composed of dense granular and occasional fibrillar non-striated material. In liquoid-injected rats 125I-fibrinogen mainly localized in kidneys and lungs, especially in the latter (P less than 0.01), and the lungs had a higher wet-to-dry weight ratios than did controls (P less than 0.01). It is concluded that the polyanion (liquoid)-induced intravascular coagulation-like reaction sequestered Fn concomitantly with the precipitation of fibrinogen and VII:Ag in the microclots. The reduced concentration of plasma Fn may have impaired the disposal of coagulation products thus enhancing the expression of the coagulopathy-mediated renal and pulmonary histopathology. It is suggested that the liquoid-related coagulopathy may have resulted in enzymatic lysis of Fn.
D000818:Animals; D001557:Benzenesulfonates / Q000494:pharmacology*; D004211:Disseminated Intravascular Coagulation / Q000097:blood / Q000139:chemically induced* / Q000473:pathology; D005340:Fibrinogen / Q000032:analysis; D005353:Fibronectins / Q000097:blood*; D007668:Kidney / Q000473:pathology; D008168:Lung / Q000473:pathology; D008297:Male; D011074:Polyanetholesulfonate / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D013927:Thrombosis / Q000473:pathology
D001557:Benzenesulfonates; D005353:Fibronectins; D011074:Polyanetholesulfonate; D005340:Fibrinogen