The importance of SH-containing substances for red blood cells in acute myeloic leukemia.
Electron spin resonance (ESR) spectra of lyophilized erythrocytes obtained from patients with acute myeloid leukemia (AML) show, in comparison to controls, a characteristic change especially in the low-field region of the spectrum concomitant with a reduction of the spin concentration. This effect can be simulated by addition of SH-containing substances (e.g. reduced glutathione or cysteine) to healthy erythrocytes. S-S containing compounds exhibit no effect. Since SH-containing substances can hardly permeate plasma membranes, the membrane surface seems to be defective in the case of "AML" erythrocytes. Furthermore, it can be concluded that the concentration of SH-containing substances, such as cysteine, is increased in the plasma of AML-patients, which could be confirmed by HPLC-measurements. In the case of a successful treatment of the patients with alexan, daunoblastin, and thioguanine the spin concentration increased again and the resulting ESR spectrum is very similar to the control spectrum. It should be pointed out, that the ascorbic acid concentration is very low in both plasma and erythrocytes of AML patients.
D001205:Ascorbic Acid / Q000097:blood; D004578:Electron Spin Resonance Spectroscopy; D004912:Erythrocytes / Q000032:analysis*; D005978:Glutathione / Q000097:blood; D006023:Glycoproteins / Q000096:biosynthesis; D006801:Humans; D007945:Leukemia, Lymphoid / Q000097:blood; D015470:Leukemia, Myeloid, Acute / Q000097:blood*; D013438:Sulfhydryl Compounds / Q000097:blood*
D006023:Glycoproteins; D013438:Sulfhydryl Compounds; D005978:Glutathione; D001205:Ascorbic Acid