[Complex formation of single-stranded phage DNA with synthetic oligodeoxynucleotides and interaction of complexes with DNA-polymerase and restriction endonuclease].
Hybridization of synthetic oligodeoxynucleotides with single-stranded phage M13mp2 DNA has been studied in terms of temperature, ionic strength, oligonucleotide molar excess and chain length, and DNA secondary structure. Combination of two decadeoxynucleotides corresponding to a nicked eicosamer (composite primer) was found to be efficient in the template-directed DNA polymerase-catalyzed chain elongation, where both decamers separately failed. Circular SS DNA was specifically linearized by BamHI cleavage of SS DNA-tetradecadeoxynucleotide duplex.
D003090:Coliphages / Q000032:analysis; D004262:DNA Restriction Enzymes; D004277:DNA, Single-Stranded / Q000032:analysis*; D004279:DNA, Viral / Q000032:analysis; D004259:DNA-Directed DNA Polymerase; D004591:Electrophoresis, Polyacrylamide Gel; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D009838:Oligodeoxyribonucleotides / Q000032:analysis*
D004277:DNA, Single-Stranded; D004279:DNA, Viral; D009838:Oligodeoxyribonucleotides; D004259:DNA-Directed DNA Polymerase; D004262:DNA Restriction Enzymes
Kompleksoobrazovanie odnotsepochechnoĭ fagovoĭ DNK s sinteticheskimi oligodezoksinukleotidami i vzaimodeĭstvie kompleksov s DNK-polimerazoi i éndonukleazoĭ restriktsii.