A mouse model for poliovirus neurovirulence identifies mutations that attenuate the virus for humans.
A mutation in the genome of poliovirus type 3 that is known to reduce neurovirulence in humans similarly reduces neurovirulence in mice when incorporated into a mouse-adapted-human poliovirus recombinant. Viral recombinants with a uracil at nucleotide position 472 in the 5'-noncoding regions of their genomes are unable to replicate in the mouse brain. Viral recombinants with a cytosine at this position are neurovirulent in mice. Neurovirulence of poliovirus in mice may therefore prove to be a useful indicator of the genetic stability of new attenuating mutations created by site-directed mutagenesis.
D000818:Animals; D001921:Brain / Q000382:microbiology*; D004195:Disease Models, Animal; D006801:Humans; D051379:Mice; D009154:Mutation; D017955:Poliovirus / Q000235:genetics / Q000472:pathogenicity*; D011054:Poliovirus Vaccine, Inactivated / Q000276:immunology; D011995:Recombination, Genetic; D014774:Virulence; D014779:Virus Replication
D011054:Poliovirus Vaccine, Inactivated