title: The structure of the promoter and amino terminal region of the pH 2.5 acid phosphatase structural gene (appA) of E. coli: a negative control of transcription mediated by cyclic AMP.
abstract: The regulatory and promoter regions of the gene for acid phosphatase of optimum pH 2.5 (appA) of Escherichia coli has been characterized by constructing appA-lacZ protein fusions. Monitoring of beta-galactosidase activity showed that the cloned fragment harbored a region that was responsible for a negative control of transcription mediated by the cAMP-cAMP receptor (CAP) complex. The nucleotide sequence of the segment was determined. A region containing a putative CAP binding site, overlapping the -10 region of the promoter was found. Deletion analysis around the promoter region, using appA-lacZ protein fusions substantiated the hypothesis for a role of the 'consensus' sequence in the negative control mediated by cyclic AMP. In addition, the coding sequence revealed the likely existence of a signal peptide similar to the one found for other exported proteins, upstream from the phosphatase protein sequence.
mesh_terms: D000135:Acid Phosphatase / Q000096:biosynthesis*; D001483:Base Sequence; D003001:Cloning, Molecular; D000242:Cyclic AMP / Q000502:physiology*; D004269:DNA, Bacterial / Q000096:biosynthesis; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D005798:Genes, Bacterial; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic; D001616:beta-Galactosidase / Q000378:metabolism; D001618:beta-Lactamases / Q000378:metabolism
chemical_list: D004269:DNA, Bacterial; D000242:Cyclic AMP; D000135:Acid Phosphatase; D001616:beta-Galactosidase; D001618:beta-Lactamases