title: Nucleotide sequence analysis of alpha-amylase and thiol protease genes that are hormonally regulated in barley aleurone cells.
abstract: We have determined the nucleotide sequences of Amy32b, a type A alpha-amylase gene, and of the gene for aleurain, a thiol protease closely related to mammalian cathepsin H. Both are expressed in barley aleurone cells under control of the plant hormones gibberellic acid and abscisic acid, but only aleurain is expressed at high levels in other barley tissues. Sequence analysis indicates that the 5' end of the aleurain gene, comprising 3 exons and 2 introns, may have become associated with the remainder of the gene, encoding the protease domain of the protein, by some sort of recombination event. This 5' domain of the gene is very G + C-rich and is flanked by inverted repetitive sequences. We found two different groups of homologous sequence elements. The first group consists of four blocks of sequences conserved in the same spatial arrangement in both genes; these are arranged at similar intervals upstream from the Amy32b TATA box and from a TATA box present in intron 3 of aleurain, outside of the 5' domain and upstream from the protease domain. A part of two of these conserved sequences is similar to the core sequence of certain enhancer elements characterized from mammalian cells. The second group of homologous elements is present in the upstream region of both genes. We speculate that these conserved sets of sequences may have some role in either the tissue specificity of expression of the genes or in some part of the hormonal regulation imposed on them.
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology*; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D003509:Cyclohexanecarboxylic Acids / Q000494:pharmacology*; D003546:Cysteine Endopeptidases; D004262:DNA Restriction Enzymes; D010450:Endopeptidases / Q000235:genetics*; D005796:Genes / Q000187:drug effects; D005809:Genes, Regulator; D005875:Gibberellins / Q000494:pharmacology*; D001467:Hordeum / Q000201:enzymology / Q000235:genetics; D010944:Plants / Q000201:enzymology / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic; D000516:alpha-Amylases / Q000235:genetics*
chemical_list: D003509:Cyclohexanecarboxylic Acids; D005875:Gibberellins; D000040:Abscisic Acid; C007842:gibberellic acid; D004262:DNA Restriction Enzymes; D000516:alpha-Amylases; D010450:Endopeptidases; D003546:Cysteine Endopeptidases; C046726:aleurain