Thrombin receptor occupancy initiates cell proliferation in the presence of phorbol myristic acetate.
A combination of DIP-thrombin and either PMA (50 ng/ml) or dioctanoyl glycerol stimulates DNA synthesis in serum free cultures of NIL hamster cells similar to that previously reported for the combinatory effect of DIP-thrombin and gamma-thrombin. Thus, PMA or dioctanoyl glycerol appears to generate signals normally stimulated by gamma-thrombin interaction with cells. This stimulation was not observed when cells were treated with DIP-thrombin and 4-beta-phorbol or 4-alpha-phorbol 12,13-didecanoate. Therefore, it appears that this effect is mediated through activation of protein kinase C and that this activation plays an important role in thrombin mitogenesis.
D000818:Animals; D002453:Cell Cycle / Q000187:drug effects*; D002460:Cell Line; D006224:Cricetinae; D004075:Diglycerides / Q000494:pharmacology; D004357:Drug Synergism; D010703:Phorbol Esters / Q000494:pharmacology; D011493:Protein Kinase C / Q000502:physiology*; D011956:Receptors, Cell Surface / Q000502:physiology*; D018179:Receptors, Thrombin; D013329:Structure-Activity Relationship; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D013917:Thrombin / Q000031:analogs & derivatives* / Q000494:pharmacology
D004075:Diglycerides; D010703:Phorbol Esters; D011956:Receptors, Cell Surface; D018179:Receptors, Thrombin; D011493:Protein Kinase C; D013917:Thrombin; C027962:diisopropylphosphoryl-thrombin; D013755:Tetradecanoylphorbol Acetate