A transcription factor which binds to the enhancers of SV40, immunoglobulin heavy chain and U2 snRNA genes.
In eukaryotes the transcriptional control of RNA polymerase II-mediated gene expression is exerted by cis-acting regulatory DNA elements classified as promoter and enhancer sequences. These elements are composed of a number of different protein binding sites. The regulatory factors that recognize such 'modules' may be ubiquitous, tissue- or stage-specific, and positively or negatively acting. According to this model the transcriptional activity of a given gene is programmed by a combination of different modules. We analysed such a site of protein-DNA interaction, the octamer motif, in the enhancers of the simian virus (SV40) early genes and the murine immunoglobulin heavy-chain gene, and in the distal sequence element (DSE) of the U2 small nuclear (sn)RNA gene of Xenopus laevis. The corresponding DNA-binding factor appears to be the same in the three cases. Moreover, a fraction containing partially purified octamer motif binding factor has a stimulatory effect on transcription in an in vitro system.
D000818:Animals; D001483:Base Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012319:RNA Polymerase II / Q000378:metabolism; D012342:RNA, Small Nuclear / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D013539:Simian virus 40 / Q000235:genetics*; D014157:Transcription Factors / Q000378:metabolism*; D014982:Xenopus laevis
D007143:Immunoglobulin Heavy Chains; D012342:RNA, Small Nuclear; D014157:Transcription Factors; D012319:RNA Polymerase II