title: The extent and distribution of skeletal muscle necrosis after graded periods of complete ischemia.
abstract: The management of an acutely ischemic extremity requires knowledge of the response of skeletal muscle (the largest component of the lower limb) to prolonged periods of complete normothermic ischemia. We have used the canine gracilis muscle model to evaluate the extent and distribution of ischemic necrosis after 3 and 5 hours of ischemia and 48 hours of reperfusion. Each muscle was cut into six slices, and the extent and distribution of postischemic necrosis identified by means of nitroblue tetrazolium staining and 99mTc pyrophosphate uptake. After 3, 4, and 5 hours of ischemia the extent of necrosis was 2.0% +/- 0.9%, 30.3% +/- 6.0%, and 90.1% +/- 3.5% (mean +/- SEM), respectively. A statistically significant correlation exists between the extent of necrosis and the uptake of 99mTc pyrophosphate uptake per gram of tissue (y = 1574.9x - 8.4, r = 0.84, p less than 0.001). Most necrosis was centrally located and found in the thickest portion of the muscle. We conclude that there is a graded response in the extent of skeletal muscle necrosis related to the length of ischemic stress rather than an "all-or-none" phenomenon. This central distribution of necrosis makes the usual external evaluation of ischemic damage clinically unreliable. In addition, since there was no enveloping fascia in this model, a compartment release alone may not prevent the development of skeletal muscle necrosis. This knowledge of the response of skeletal muscle to ischemia may lead to an improved clinical approach to an extremity suffering a prolonged ischemic insult.
mesh_terms: D000818:Animals; D011756:Diphosphates; D004285:Dogs; D007511:Ischemia / Q000000981:diagnostic imaging / Q000473:pathology*; D009132:Muscles / Q000098:blood supply / Q000000981:diagnostic imaging / Q000473:pathology*; D009336:Necrosis; D009580:Nitroblue Tetrazolium; D011877:Radionuclide Imaging; D013667:Technetium; D016698:Technetium Tc 99m Pyrophosphate; D013997:Time Factors; D014463:Ultrasonography; D014654:Vascular Patency
chemical_list: D011756:Diphosphates; D009580:Nitroblue Tetrazolium; D016698:Technetium Tc 99m Pyrophosphate; D013667:Technetium