Search for HSV DNA in genital, cerebral and labial tumors.
DNA sequences homologous to HSV-1 and HSV-2 DNA fragments were searched in 64 genital, 35 labial and 34 cerebral tumors. Southern blot transfers of tumor and control DNAs were hybridized in stringent conditions with 32P labelled probes from HSV-1 and HSV-2 cloned DNA fragments. Specific hybridization to HSV-2 BglII N fragment was observed in six (9.4%) genital tumors. Labial and cerebral tumors did not show hybridization to any of the probes used. The technique employed allowed the detection of 0.1 copies of viral fragments per diploid genome.
D001483:Base Sequence; D001932:Brain Neoplasms / Q000032:analysis*; D004279:DNA, Viral / Q000302:isolation & purification*; D005260:Female; D005833:Genital Neoplasms, Female / Q000032:analysis*; D006801:Humans; D008048:Lip Neoplasms / Q000032:analysis*; D009693:Nucleic Acid Hybridization; D012689:Sequence Homology, Nucleic Acid; D018139:Simplexvirus
D004279:DNA, Viral