title: Fructofuranose 2-phosphate is the product of dephosphorylation of fructose 2,6-bisphosphate.
abstract: Using comparative ion-exchange chromatography on Dowex 1X4, the product of dephosphorylation of fructose 2,6-bisphosphate with purified yeast fructose-2,6-bisphosphate 6-phosphohydrolase, was shown to be identical to the furanose form of fructose 2-phosphate prepared by chemical synthesis according to Pontis and Fischer [Biochem. J. 89, 452-459 (1963)]. As expected for the furanose form of fructose 2-phosphate, the enzymatically formed product consumes 1 mol periodate/mol fructose 2-phosphate, whereas the chemically synthesized pyranose form consumes 2 mol periodate/mol. In addition, it is shown that the enzymatic product behaves identically to the furanose, not the pyranose, form of fructose 2-phosphate in hydrolysis of the ester bond at pH 4 and 37 degrees C, as described previously for the chemically synthesized compounds [Pontis and Fischer (1963) vide supra].
mesh_terms: D002852:Chromatography, Ion Exchange; D005635:Fructosediphosphates / Q000378:metabolism*; D005636:Fructosephosphates / Q000096:biosynthesis*; D006598:Hexosediphosphates / Q000378:metabolism*; D006868:Hydrolysis; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology
chemical_list: D005635:Fructosediphosphates; D005636:Fructosephosphates; D006598:Hexosediphosphates; C052127:fructose-2-phosphate; C027652:fructose 2,6-diphosphate; D010744:Phosphoric Monoester Hydrolases; C085449:fructose-2,6-bisphosphate 6-phosphatase