title: Localization of the synthesis of very-long-chain fatty acid in mitochondria from Saccharomyces cerevisiae.
abstract: The localization of the mitochondrial elongation activities ('elongases') from Saccharomyces cerevisiae has been investigated. It was shown, using carboxyatractyloside in the incubation mixture, that synthesis of very-long-chain fatty acids probably occurred outside the matrix and, by fractionation experiments, that elongases are membrane-bound enzymes. The solubilization of the outer membrane by digitonin showed that three elongating activities are correlated with a marker of the outer membrane and not with an inner membrane marker. A further partial purification of the outer membrane showed that elongases are present in the outer membrane of mitochondria.
mesh_terms: D051080:Acyl-Carrier Protein S-Malonyltransferase; D000217:Acyltransferases / Q000302:isolation & purification / Q000378:metabolism*; D001278:Atractyloside / Q000031:analogs & derivatives / Q000494:pharmacology; D001665:Binding Sites; D002499:Centrifugation, Density Gradient; D004072:Digitonin / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D005227:Fatty Acids / Q000096:biosynthesis*; D007425:Intracellular Membranes / Q000201:enzymology; D008928:Mitochondria / Q000201:enzymology / Q000378:metabolism*; D010101:Oxygen Consumption; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D013395:Sucrose
chemical_list: D005227:Fatty Acids; D001278:Atractyloside; D013395:Sucrose; D000217:Acyltransferases; D051080:Acyl-Carrier Protein S-Malonyltransferase; D004072:Digitonin; C003853:carboxyatractyloside