title: Cyclic 3':5'-adenosine monophosphate phosphodiesterase and cyclic 3':5'-guanosine monophosphate phosphodiesterase of normal granulocytes and granulocytes of chronic myelogenous leukaemia.
abstract: Normal human neutrophilic granulocytes and granulocytes of chronic myelogenous leukaemia (CML) were shown to possess only the "high Km type" isoenzyme of cAMP-phosphodiesterase. The properties of this enzyme are similar in both normal and CML granulocytes. cGMP-phosphodiesterase in human granulocytes was found to be composed of "high Km type" and "low Km type" isoenzymes. The high Km cGMP-phosphodiesterase of CML granulocytes showed a considerably lower apparent Km and Vm value than those in normal neutrophilic granulocytes.
mesh_terms: D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000097:blood*; D015106:3',5'-Cyclic-GMP Phosphodiesterases / Q000097:blood*; D003600:Cytosol / Q000201:enzymology; D005260:Female; D006098:Granulocytes / Q000201:enzymology*; D006801:Humans; D007527:Isoenzymes / Q000097:blood; D007951:Leukemia, Myeloid / Q000097:blood / Q000201:enzymology*; D008297:Male
chemical_list: D007527:Isoenzymes; D015105:3',5'-Cyclic-AMP Phosphodiesterases; D015106:3',5'-Cyclic-GMP Phosphodiesterases