Comparison of reaction rates in trypsin-catalysed transamidation of porcine insulin and its B29-arginine analogue.
[B29-Arginine]porcine insulin was prepared from des-(B23-30)-insulin and synthetic octapeptide with the aid of trypsin. Comparison of reaction rates in trypsin-catalysed transamidation of this compound and porcine insulin with threonine ether ester showed that this reaction is determined only by conformational effects and structural features of amino acids leaving from and entering into B30, not by the structure and the pKa value of the basic amino acid in B29.
D000577:Amides; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D007328:Insulin / Q000031:analogs & derivatives*; D007700:Kinetics; D013379:Substrate Specificity; D013552:Swine; D014357:Trypsin / Q000378:metabolism*
D000577:Amides; D000596:Amino Acids; D007328:Insulin; C056863:insulin, Arg(B29)-; D014357:Trypsin