Effects of gamma-irradiation on the erythrocyte membrane: ESR, NMR and biochemical studies.
The effects of gamma-irradiation on resealed erythrocyte ghosts have been examined with different techniques. Phospholipid analysis reveals peroxidative damage on the polyunsaturated chains of phosphatidylethanolamine. Gel electrophoresis and ESR measurements indicate modifications of the cytoskeletal proteins. 31P Nuclear magnetic resonance data show bilayer modifications that can be interpreted as changes in lipid-protein interactions. The overall picture from the present results favours interaction between lipids and proteins in the inner monolayer of the membrane.
D003037:Cobalt Radioisotopes; D003598:Cytoskeletal Proteins / Q000528:radiation effects; D004578:Electron Spin Resonance Spectroscopy; D004910:Erythrocyte Membrane / Q000528:radiation effects*; D005720:Gamma Rays; D006801:Humans; D066298:In Vitro Techniques; D008054:Lipid Peroxides / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008563:Membrane Lipids / Q000528:radiation effects
D003037:Cobalt Radioisotopes; D003598:Cytoskeletal Proteins; D008054:Lipid Peroxides; D008563:Membrane Lipids