[Characteristics of endogenous proteolysis in preparations of human erythrocyte membranes].
Endoproteolytic activity in human erythrocyte membrane preparations has been examined at 37 degrees C by one- and two-dimensional electrophoresis. Two-dimensional mapping has shown that the presence of leukocyte enzymes in erythrocytes prepared in a regular manner (centrifugation) cannot be excluded. Sedimentation in the 1.5% dextran 500,000 with the following erythrocyte purification on HBS-cellulose has made it possible to prepare erythrocyte membranes characterized by low level endoproteolytic activity without leukocyte enzymes. The marker peptide has been found. It is likely to be a specific product of the enzyme activity of membrane localization.
D002458:Cell Fractionation; D015180:Electrophoresis, Gel, Two-Dimensional; D004910:Erythrocyte Membrane / Q000378:metabolism*; D006801:Humans; D006868:Hydrolysis; D008565:Membrane Proteins / Q000097:blood*; D010447:Peptide Hydrolases / Q000097:blood* / Q000302:isolation & purification
D008565:Membrane Proteins; D010447:Peptide Hydrolases
Kharakteristika éndogennogo proteoliza v preparatakh membran éritrotsitov cheloveka.