Prostaglandin E2 directly protects isolated rat gastric surface cell membranes against bile salts.
Rat gastric surface cell membranes were prepared and the effect of taurocholic acid assessed by ESR spectroscopy using the 16-doxylstearic acid spin label. Taurocholic acid increased the polar part of the spectra, indicating an augmented amount of spin label molecules with a polar environment. Concomitantly, mobility of the spin label molecule was augmented. The effect of taurocholic acid was completely prevented by the previous addition of prostaglandin E2. This suggests a direct protective efficiency of prostaglandin E2 on rat gastric surface cell membranes without the metabolic participation of intact cells.
D000818:Animals; D001647:Bile Acids and Salts / Q000633:toxicity*; D002462:Cell Membrane / Q000187:drug effects; D015232:Dinoprostone; D004578:Electron Spin Resonance Spectroscopy; D005260:Female; D005753:Gastric Mucosa / Q000187:drug effects*; D066298:In Vitro Techniques; D011458:Prostaglandins E / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains
D001647:Bile Acids and Salts; D011458:Prostaglandins E; D015232:Dinoprostone