Alpha-actinin-containing aggregates in transformed cells are highly dynamic structures.
Normal rat kidney cells infected with a Rous sarcoma virus (strain LA23) were used to study the dynamics of alpha-actinin-containing aggregates in transformed cells. Experiments were performed by microinjecting living cells with iodoacetamidotetramethylrhodamine alpha-actinin and allowing the fluorescent analogue to incorporate into cellular structures. Subsequent time-lapse recording indicated that the alpha-actinin-containing aggregates can undergo rapid formation, movement, and breakdown. In addition, experiments using the photobleaching recovery technique indicated that alpha-actinin molecules associated with the aggregates have a very high rate of exchange, whereas those associated with adhesion plaques in normal cells exchange much more slowly. The dynamic properties of alpha-actinin-containing aggregates may be closely related to the changes in cellular behavior upon oncogenic transformation.
D000185:Actinin / Q000378:metabolism*; D000818:Animals; D001358:Avian Sarcoma Viruses / Q000502:physiology; D002448:Cell Adhesion; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002472:Cell Transformation, Viral; D008856:Microscopy, Fluorescence; D051381:Rats
D000185:Actinin