Metabolic and secretory response of tumoral-insulin producing cells to D-fructose and D-galactose.
At variance with normal islet cells, tumoral insulin-producing cells of the RINm5F line were found to display a positive secretory response not solely to D-glucose and D-mannose, but also to D-fructose and D-galactose. All hexoses increased the ATP/ADP ratio, exerted a sparing action upon the oxidation of endogenous nutrients in cells prelabelled with either L-[U-14C]glutamine or [U-14C]palmitate, increased the output of lactic acid and, as judged from data collected in the presence of D-[U-14C]hexoses, underwent oxidation in the RINm5F cells. The secretory response to these four hexoses appeared commensurate with the extent of their metabolic effects.
D000227:Adenine Nucleotides / Q000378:metabolism; D007516:Adenoma, Islet Cell / Q000378:metabolism*; D000818:Animals; D004734:Energy Metabolism; D005632:Fructose / Q000502:physiology*; D005690:Galactose / Q000502:physiology*; D005973:Glutamine / Q000378:metabolism; D006601:Hexoses / Q000378:metabolism; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007340:Insulinoma / Q000378:metabolism*; D007773:Lactates / Q000378:metabolism; D008358:Mannose / Q000502:physiology*; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000378:metabolism; D011773:Pyruvates / Q000378:metabolism; D051381:Rats; D013806:Theophylline / Q000494:pharmacology; D014867:Water / Q000378:metabolism
D000227:Adenine Nucleotides; D006601:Hexoses; D007328:Insulin; D007773:Lactates; D010169:Palmitic Acids; D011773:Pyruvates; D014867:Water; D005973:Glutamine; D019308:Palmitic Acid; D005632:Fructose; D013806:Theophylline; D008358:Mannose; D005690:Galactose