[Synthesis of phosphonate analogs of sphingomyelins and preparation of an affinity sorbent for sphingomyelinase purification].
Phosphonate analogues of 2-N-stearoyl- (I) and 2-N-(undec-10-enoyl)-sphingomyelins (II) have been synthesised. Compound (II) was used as a starting product for preparation of a sorbent for sphingomyelinase affinity chromatography. The double bond of the unsaturated undec-10-enoyl moiety of the phosphonate analogue (II) was oxidized, and the modified (II) was coupled to amino-Toyopearl HW-65 to give a sorbent containing 4 mumoles of ligand per milliliter of the swollen resin.
D001409:Bacillus cereus / Q000201:enzymology; D055598:Chemical Phenomena; D002621:Chemistry; D002846:Chromatography, Affinity; D008024:Ligands; D009943:Organophosphorus Compounds / Q000138:chemical synthesis*; D010727:Phosphoric Diester Hydrolases / Q000302:isolation & purification*; D013108:Sphingomyelin Phosphodiesterase / Q000302:isolation & purification*; D013109:Sphingomyelins / Q000138:chemical synthesis*
D008024:Ligands; D009943:Organophosphorus Compounds; D013109:Sphingomyelins; D010727:Phosphoric Diester Hydrolases; D013108:Sphingomyelin Phosphodiesterase
Sintez fosfonovykh analogov sfingomielinov i poluchenie affinnogo sorbenta dlia ochistki sfingomielinaz.