Studies on DNA topoisomerases I and II in herpes simplex virus type 2-infected cells.
It has been suggested that herpes simplex virus (HSV) type 1 may induce a virus-specific DNA topoisomerase activity which copurifies with virus-induced DNA polymerase. We have examined DNA topoisomerase (TOPO) I and II activities in HSV-2-infected HeLa S3 cells. Both activities were partially purified using DEAE-cellulose, phosphocellulose and double-stranded DNA cellulose column chromatography. It was found that both activities could be separated from HSV-2-specific DNA polymerase. Throughout the purification TOPO I could be immunologically detected with a monoclonal antibody developed against human TOPO I. Regardless of the source, mock- or HSV-2-infected human cells, both types of topoisomerase were equally tolerant of 200 mM-KCl. There appeared to be no apparent heterogeneity of TOPO I in HeLa S3 cells through the course of the HSV-2 infection. We conclude that host cell topoisomerases are quite stable in HSV-2-infected HeLa S3 cells and that there is no evidence that HSV-2 is capable of inducing HSV-2-specific TOPO I and TOPO II activities.
D002472:Cell Transformation, Viral; D004264:DNA Topoisomerases, Type I / Q000096:biosynthesis* / Q000302:isolation & purification; D004250:DNA Topoisomerases, Type II / Q000096:biosynthesis* / Q000302:isolation & purification; D004259:DNA-Directed DNA Polymerase / Q000302:isolation & purification / Q000378:metabolism; D004790:Enzyme Induction; D006367:HeLa Cells / Q000201:enzymology; D006801:Humans; D007700:Kinetics; D018139:Simplexvirus / Q000201:enzymology*
D004259:DNA-Directed DNA Polymerase; D004264:DNA Topoisomerases, Type I; D004250:DNA Topoisomerases, Type II