The poliovirus-induced shut-off of cellular protein synthesis persists in the presence of 3-methylquercetin, a flavonoid which blocks viral protein and RNA synthesis.
In poliovirus-infected cells, the viral protein and RNA synthesis were severely reduced, provided 3-methylquercetin was present between 1 and 2 h post-infection. Under these conditions, the virally induced host shut-off remained in effect. On the other hand, in uninfected HeLa cells, protein and RNA synthesis was inhibited only slightly by 3-methylquercetin. The inhibition of poliovirus cytopathogenicity in Vero cells by 3-methylquercetin exhibited a similar time dependence.
D000818:Animals; D003588:Cytopathogenic Effect, Viral / Q000187:drug effects; D004591:Electrophoresis, Polyacrylamide Gel; D005419:Flavonoids / Q000494:pharmacology*; D044948:Flavonols; D006367:HeLa Cells; D006801:Humans; D017955:Poliovirus / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D014176:Protein Biosynthesis; D011794:Quercetin / Q000031:analogs & derivatives / Q000494:pharmacology*; D012367:RNA, Viral / Q000096:biosynthesis* / Q000187:drug effects; D014709:Vero Cells; D014764:Viral Proteins / Q000096:biosynthesis*
D005419:Flavonoids; D044948:Flavonols; D012367:RNA, Viral; D014764:Viral Proteins; C047368:3-methylquercetin; D011794:Quercetin