In vitro effects of calcium phosphate biomaterials on fibroblastic cell behavior.
The effect of synthetic granular hydroxyapatite (HAP) on cultured fibroblastic cells (L929, human bone and gingiva cells) was studied. Phagocytosis of HAP particles and resulting morphological cell changes were demonstrated by microscopic examinations. Cell counts and [3H]thymidine uptake indicated significant increases in cell proliferation and DNA synthesis. These results could account for some of the alterations of the fibroblast behavior induced by changes in intracellular levels of calcium ions released from the material.
D000818:Animals; D001672:Biocompatible Materials; D001842:Bone and Bones / Q000166:cytology; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D017886:Durapatite; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure; D005881:Gingiva / Q000166:cytology; D006801:Humans; D006882:Hydroxyapatites / Q000494:pharmacology*; D007739:L Cells / Q000166:cytology; D051379:Mice; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D010587:Phagocytosis
D001672:Biocompatible Materials; D006882:Hydroxyapatites; D017886:Durapatite