Desalting and concentration of proteins in dilute solution using reversed-phase high-performance liquid chromatography.
A rapid method for desalting and concentrating dilute protein solutions using short reversed-phase columns (3-4 cm) has been described. The recovery of proteins is usually 90-100%. The method is simple and rapid and allows the desalting and concentration of protein samples simultaneously. A wide variety of proteins in the range up to 80 kDa can be desalted in microgram to milligram amounts, and volumes up to 1 liter can be concentrated to a few milliliters by a single injection.
D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003574:Cytochrome c Group / Q000302:isolation & purification; D009113:Muramidase / Q000302:isolation & purification; D011506:Proteins / Q000302:isolation & purification*; D012492:Salts / Q000302:isolation & purification; D012710:Serum Albumin, Bovine; D012996:Solutions
D003574:Cytochrome c Group; D011506:Proteins; D012492:Salts; D012996:Solutions; D012710:Serum Albumin, Bovine; D009113:Muramidase