title: Superiority of antibody versus delayed hypersensitivity in clearance of HSV-1 from eye.
abstract: The contribution that antibody and delayed type hypersensitivity (DTH) make in promoting HSV-1 clearance from the infected cornea was investigated. Balb/c mice were immunized intravenously or subcutaneously with an attenuated strain of HSV-1 to generate hosts which were antibody-producing DTH-tolerant or antibody-producing DTH-responsive. Anti-mu serum treated mice were likewise sensitized intravenously or subcutaneously to obtain hosts which were antibody depressed-DTH tolerant or antibody-depressed DTH-responsive. Eight days after sensitization, these four sensitized groups and unsensitized controls were infected on scarified corneas with a stromal keratitis inducing strain of HSV-1, and the extent of virus replication was determined 1, 3, and 7 days later. Very different results were obtained depending upon the host's immune status. Virus proliferated extensively (greater than 3-4 logs) in the eyes of nonimmune mice and antibody-depressed DTH-tolerant hosts during the first 3 days after infection. In striking contrast, HSV-1 could not be detected even 24 hr post challenge in antibody-producing DTH-tolerant mice. In fact, such mice cleared virus from the eye as efficiently as immunologically intact hosts. However, in mice with the reverse immune status, ie antibody-depressed DTH-responsive, virus growth was clearly evident (greater than 2-3 logs) during days 1-3, and only thereafter did complete clearance occur. These results indicate that in the sensitized host antibody is both independent of and significantly more effective than DTH in promoting HSV-1 eradication from the infected eye.
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology*; D000917:Antibody Formation; D000956:Antigens, Viral / Q000276:immunology; D005123:Eye / Q000276:immunology*; D006968:Hypersensitivity, Delayed / Q000276:immunology*; D007154:Immune System Diseases / Q000276:immunology; D007109:Immunity; D007635:Keratitis, Dendritic / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D018139:Simplexvirus / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral