title: Poliovirus infection of established human blood cell lines: relationship between the differentiation stage and susceptibility of cell killing.
abstract: The replication of type 1 poliovirus in 13 established human blood cell lines differing in the differentiation stage and cell lineage was investigated. Three T (CCRF-CEM, CCRF-HSB-2, and Molt-3) and three B (Raji, CCRF-SB, and RPMI 8226) cell lines showed no cytopathic effects (CPE) or virus production. CPE associated with virus production were detected in the other seven cell lines: HL-60, ML-1, and KG-1 (granulocytic lineage), U-937 and THP-1 (monocytic lineage), K-562 (erythroid lineage), and Molt-4 (T cell lineage). These susceptible cell lines greatly differed in the speed at which the CPE progressed. The progression of CPE was faster in relatively well-differentiated cell lines such as HL-60 and U-937, independently of the multiplicity of infection, than in less differentiated cell lines such as K-562, KG-1, and THP-1. Thus, for the same lineage, the speed at which CPE progressed became proportionally higher with subsequent differentiation stages. In the K-562 cell culture, CPE were not observed until at least 5 days postinfection (p.i.), while more than 80% of HL-60 cells were killed within 3 days p.i. There were no significant differences between infected HL-60 and K-562 cells in the efficiency of infection determined at 8 hr p.i. by the indirect immunofluorescent technique, the rate of virus growth, or the amount of viral capsid protein synthesized. This indicated that there were similar viral replication cycles in the two cell lines. These observations suggest that the killing function of the virus is expressed more slowly in K-562 cells than in HL-60 cells.
mesh_terms: D001773:Blood Cells / Q000166:cytology / Q000382:microbiology*; D002213:Capsid / Q000096:biosynthesis; D002454:Cell Differentiation; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D004900:Erythroblasts / Q000382:microbiology; D006098:Granulocytes / Q000382:microbiology; D006367:HeLa Cells / Q000382:microbiology; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000382:microbiology*; D006801:Humans; D008214:Lymphocytes / Q000382:microbiology; D009000:Monocytes / Q000382:microbiology; D017955:Poliovirus / Q000254:growth & development*; D014779:Virus Replication