[Detection of HTLV-III-specific sequences in T-lymphocyte lines by molecular hybridization].
The methods of molecular hybridization are described using 32P-labeled plasmid pBHIO carrying the cloned HTLV-III provirus employed for the detection of HTLV-III-specific sequences in lines of T-lymphocytes from patients with acquired immune deficiency syndrome. The presence of virus-specific sequences was demonstrated in 13 out of 20 lines examined by the dot-blot hybridization method. The blot-hybridization method revealed differences in sites of Hind III restriction in 2 out of 6 lines studied. The possibility of combined infection of line No. 17 with two different variants of HTLV-III is discussed.
D002460:Cell Line; D003001:Cloning, Molecular / Q000379:methods; D004262:DNA Restriction Enzymes / Q000494:pharmacology; D004279:DNA, Viral / Q000187:drug effects / Q000235:genetics*; D015247:Deoxyribonuclease HindIII; D006678:HIV / Q000187:drug effects / Q000235:genetics*; D006801:Humans; D009693:Nucleic Acid Hybridization / Q000187:drug effects; D010761:Phosphorus Radioisotopes; D010957:Plasmids; D013601:T-Lymphocytes / Q000382:microbiology*
D004279:DNA, Viral; D010761:Phosphorus Radioisotopes; D004262:DNA Restriction Enzymes; D015247:Deoxyribonuclease HindIII
Vyiavlenie HTLV-III-spetsificheskikh posledovatel'nosteĭ v liniiakh T-limfotsitov metodom molekuliarnoĭ gibridizatsii.