title: Fixation and immunofluorescent analysis of creatine kinase isozymes in embryonic skeletal muscle.
abstract: Pectoral muscles from chicken embryos of various ages were examined with immunofluorescent and radiolabeled probes for the presence of brain-type creatine kinase (B-CK), muscle-specific creatine kinase (M-CK), muscle-specific myosin heavy chain (MHC), and cycling cells. The diffusible creatine kinase isozymes were not detectable by indirect immunofluorescence after standard histological fixation of embryonic muscle. However, a fixation procedure was devised that permitted immunodetection of the creatine kinase isozymes (particularly B-CK) in embryonic tissue from all stages of development studied. B-CK, M-CK, and MHC were all detected in post-mitotic muscle cells, but only B-CK was detected in cycling cells. Correlations between these findings and in vitro observations of a deterministic muscle lineage are discussed.
mesh_terms: D000818:Animals; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D002454:Cell Differentiation; D002642:Chick Embryo; D003402:Creatine Kinase / Q000032:analysis*; D005404:Fixatives; D005455:Fluorescent Antibody Technique; D006651:Histocytochemistry; D007527:Isoenzymes; D009124:Muscle Proteins / Q000032:analysis; D009132:Muscles / Q000196:embryology* / Q000201:enzymology; D009218:Myosins / Q000032:analysis; D010770:Phosphotransferases / Q000032:analysis; D015800:Protozoan Proteins
chemical_list: D005404:Fixatives; D007527:Isoenzymes; D009124:Muscle Proteins; D015800:Protozoan Proteins; D010770:Phosphotransferases; D017871:Calcium-Calmodulin-Dependent Protein Kinases; C033142:myosin-heavy-chain kinase; D003402:Creatine Kinase; D009218:Myosins