Schwann cell-conditioned medium supports neurite outgrowth and survival of spinal cord neurons in culture.
The effect of Schwann cell-conditioned medium (SCM) on the development in vitro of spinal cord neurons was studied. Spinal cord neurons from 18-day-old rat embryos were cultured in serum-free conditioned medium obtained from confluent rat Schwann cells. In cultures fed SCM, the cells developed typical neuronal morphology and were identified by indirect immunofluorescence using a monoclonal antibody to neurofilament protein. SCM stimulated neurite outgrowth and supported survival of spinal cord neurons. Preliminary characterization suggests that the neurotrophic factor in SCM appears to be a protein with a molecular weight greater than 8000 daltons.
D000818:Animals; D001369:Axons / Q000502:physiology*; D002470:Cell Survival; D002478:Cells, Cultured; D003470:Culture Media; D005455:Fluorescent Antibody Technique; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D012583:Schwann Cells / Q000378:metabolism*; D013116:Spinal Cord / Q000166:cytology* / Q000196:embryology
D003470:Culture Media