Genomic clone of hst with transforming activity from a patient with acute leukemia.
We have previously reported the identification of a novel transforming gene, hst, in DNA samples taken from human stomach cancers and a noncancerous portion of stomach. Five clones, containing the genomic hst gene, were isolated from a human cosmid library constructed from leukocyte DNA from a patient with acute leukemia. All clones possessed transforming activity when transfected to NIH3T3 cells. From one clone, an 8.7 kb BamHI fragment was subcloned into pBR322, and this subclone was active in transforming NIH3T3 cells. This is the first isolation of transformation-competent genomic hst clones directly from a human genomic library, that is, without prior passage through NIH3T3 cells.
D000208:Acute Disease; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D004247:DNA / Q000235:genetics*; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant; D015245:Deoxyribonuclease BamHI; D006801:Humans; D007938:Leukemia / Q000235:genetics*; D007962:Leukocytes / Q000032:analysis; D009693:Nucleic Acid Hybridization; D014162:Transfection
D004274:DNA, Recombinant; D004247:DNA; D004262:DNA Restriction Enzymes; D015245:Deoxyribonuclease BamHI