12-O-tetradecanoylphorbol-13-acetate activates phosphatidylethanol and phosphatidylglycerol synthesis by phospholipase D in cell lysates.
A cell-free system for the synthesis of phosphatidylalcohols was developed in sonicates of HL-60 cells. With [32P]phosphatidylcholine as the exogenous substrate, both phosphatidylethanol and phosphatidylglycerol were formed through a phospholipase D-catalyzed transphosphatidylation of ethanol and glycerol, respectively. The transphosphatidylation by phospholipase D was stimulated in vitro by 12-O-tetradecanoylphorbol-13-acetate (TPA) and required the addition of ATP for an optimal response. GTP-gamma-S, an activator of G protein systems, also stimulated the process by an independent mechanism. It is postulated that the stimulation of phospholipid metabolism through phospholipase D activation represents an important mechanism whereby TPA might modulate intracellular signal generating systems or influence the activity of membrane-bound proteins by altering their lipid environment.
D002458:Cell Fractionation; D002460:Cell Line; D004789:Enzyme Activation / Q000187:drug effects; D020404:Glycerophospholipids; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D006160:Guanosine Triphosphate / Q000031:analogs & derivatives / Q000494:pharmacology; D006801:Humans; D010712:Phosphatidic Acids / Q000096:biosynthesis*; D010715:Phosphatidylglycerols / Q000096:biosynthesis*; D010739:Phospholipase D / Q000502:physiology*; D010740:Phospholipases / Q000502:physiology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D013873:Thionucleotides / Q000494:pharmacology; D014461:Ultracentrifugation
D020404:Glycerophospholipids; D010712:Phosphatidic Acids; D010715:Phosphatidylglycerols; D013873:Thionucleotides; C051521:phosphatidylethanol; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D006160:Guanosine Triphosphate; D010740:Phospholipases; D010739:Phospholipase D; D013755:Tetradecanoylphorbol Acetate