Cultivation of avian rotaviruses in chicken lymphocytes and lymphoblastoid cell lines.
Avian rotavirus isolates were used to infect normal chicken spleen cells, lymphoblastoid T cell lines transformed by Marek's disease virus, an avian leukosis virus-transformed B cell line, and a reticuloendotheliosis virus-transformed line, which is a pre-B, pre-T cell line. All five isolates tested were able to infect spleen cells and the three types of lymphoblastoid cell lines, suggesting that avian rotaviruses can infect both B and T cells. Splenic lymphocytes were considerably less susceptible to infection than chick kidney cells. Lymphoblastoid cell lines remained virus-positive during a 10-day culture period. Virus was neutralized by the addition of low dilutions of normal chicken serum and high dilutions of chicken anti-rotavirus serum.
D000818:Animals; D001402:B-Lymphocytes / Q000382:microbiology; D001769:Blood; D002460:Cell Line; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D002645:Chickens; D008214:Lymphocytes / Q000382:microbiology*; D012401:Rotavirus / Q000502:physiology*; D013154:Spleen; D013601:T-Lymphocytes / Q000382:microbiology; D013696:Temperature; D014357:Trypsin; D014779:Virus Replication
D014357:Trypsin