Assaying for superoxide dismutase activity: some large consequences of minor changes in conditions.
Most assays for superoxide dismutase depend upon competition between the enzyme and some indicating scavenger for O-2. We have investigated the effects of experimental variables on assays based upon the use of either ferricytochrome c or nitro blue tetrazolium. Our results should help investigators to avoid the numerous potential pitfalls which necessarily surround these assay methods.
D000818:Animals; D002417:Cattle; D003574:Cytochrome c Group; D006863:Hydrogen-Ion Concentration; D009580:Nitroblue Tetrazolium; D013482:Superoxide Dismutase / Q000032:analysis*; D014969:Xanthine Oxidase
D003574:Cytochrome c Group; D009580:Nitroblue Tetrazolium; D013482:Superoxide Dismutase; D014969:Xanthine Oxidase