Ki-M8 monoclonal antibody reactive with an intracytoplasmic antigen of monocyte/macrophage lineage.
A monoclonal antibody (MoAb), Ki-M8, that reacts specifically with cells of the monocyte/macrophage system is described. On light and electron microscopic immunohistochemistry, Ki-M8 recognizes intracytoplasmatically localized antigens of mol wt 30,000 and 32,000, increasingly expressed during differentiation of monocytes into macrophages. Ki-M8 antigen is detectable on almost all known tissue macrophages and monocyte/macrophage-related cell lines after appropriate stimulation. In functional terms Ki-M8 significantly impairs the generation of oxygen radicals during an induced respiratory burst. Applied to acute nonlymphoblastic leukemias, a clear-cut differentiation of the monocytic phenotype and differentiation is possible on the basis of Ki-M8 immunoreactivity. Ki-M8 represents a reagent specific for the monocyte/macrophage system with regard to antigen distribution in normal and neoplastic cells as well as with regard to its influence on a typical monocyte/macrophage-related function.
D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D000954:Antigens, Surface / Q000032:analysis*; D002454:Cell Differentiation; D006651:Histocytochemistry; D006801:Humans; D007124:Immunoenzyme Techniques; D007425:Intracellular Membranes / Q000032:analysis*; D007951:Leukemia, Myeloid / Q000276:immunology / Q000473:pathology; D008163:Luminescent Measurements / Q000379:methods; D008247:Lysosomes / Q000032:analysis; D008264:Macrophages / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D008854:Microscopy, Electron; D009000:Monocytes / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D013481:Superoxides / Q000378:metabolism
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D013481:Superoxides