title: Detection of some retinoid radicals using high-performance liquid chromatography with electron spin resonance spectroscopy or electrochemical detection.
abstract: Radical species were detected in the incubation mixtures of some retinoids (retinoic acid, retinal, retinol and retinyl acetate) by using the spin-trapping technique. The spin-adducts were resolved by high-performance liquid chromatography on a reversed-phase column with isocratic elution and detected by electron spin resonance spectroscopy and electrochemical detection. The spin-adducts were eluted in the order retinoic acid, retinol, retinyl acetate, in a similar manner to the retinoids themselves. These results suggest that the spin-adducts are products of nitrosobenzene with retinoid radicals in which the retinoid radicals retain their original chemical structures.
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D004578:Electron Spin Resonance Spectroscopy; D005609:Free Radicals; D009603:Nitroso Compounds / Q000032:analysis; D012176:Retinoids / Q000032:analysis*
chemical_list: D005609:Free Radicals; D009603:Nitroso Compounds; D012176:Retinoids; C012456:nitrosobenzene