Derivation of clones close to met by preparative field inversion gel electrophoresis.
The molecular analysis of genes identified by mutations is a major problem in mammalian genetics. As a step toward this goal, preparative field inversion gel electrophoresis (FIGE) was used to selectively isolate clones from the environment of genetically linked markers, and to select a subset of these clones containing sequences next to specific restriction sites rare in mammalian DNA. This approach has been used to generate a library highly enriched in sequences closely linked to the cystic fibrosis marker met. One clone derived from the end of a Not I restriction fragment containing the met sequence was analyzed in detail and localized within a long range map to a position 300 kilobase pairs 5' of the metD sequence.
D010582:Bacteriophage lambda; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003550:Cystic Fibrosis / Q000235:genetics*; D004262:DNA Restriction Enzymes; D004586:Electrophoresis / Q000379:methods*; D005819:Genetic Markers; D005822:Genetic Vectors; D006801:Humans; D009154:Mutation; D009693:Nucleic Acid Hybridization; D012091:Repetitive Sequences, Nucleic Acid
D005819:Genetic Markers; D004262:DNA Restriction Enzymes