Human trophoblast glycoproteins defined by monoclonal antibody 1D2.
A cell surface antigen has been defined by a monoclonal antibody 1D2, raised following immunisation with lectin-purified syncytiotrophoblast glycoproteins. 1D2 was nonreactive with any one of 8 common trophoblast proteins in immunodot. Analysis of nonreduced western blots of syncytiotrophoblast microvillous plasma membrane (StMPM) protein indicated that mAb 1D2 was reactive with a series of sialylated proteins with molecular weights of 16-22 kilodaltons. Immunoprecipitates of radiolabelled StMPM protein contained molecules that co-migrated with placental alkaline phosphatase in addition to those identified by western blotting. This set of human trophoblast molecules has not been previously identified by monoclonal antibodies; the antigenicity is widely distributed in human tissues.
D000469:Alkaline Phosphatase / Q000032:analysis; D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D002850:Chromatography, Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005455:Fluorescent Antibody Technique; D006023:Glycoproteins / Q000032:analysis*; D006801:Humans; D007124:Immunoenzyme Techniques; D007158:Immunologic Techniques; D010920:Placenta / Q000201:enzymology; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000032:analysis*; D014327:Trophoblasts / Q000032:analysis*
D000911:Antibodies, Monoclonal; D006023:Glycoproteins; D011257:Pregnancy Proteins; D000469:Alkaline Phosphatase