title: (2')3',5'-Bisphosphate nucleotidase.
abstract: (2')3',5'-Bisphosphate nucleotidase has been prepared in electrophoretically homogeneous form from guinea pig liver. The enzyme catalyzes the hydrolysis of the 2'- or 3'-phosphate from the appropriate nucleoside 2',5'- and 3',5'-bisphosphates and is active with 3'-phosphoadenosine 5'-phosphosulfate and with coenzyme A but not with ATP. The 40,000-dalton protein is a monomer that requires Mg2+ for activity.
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002846:Chromatography, Affinity; D002848:Chromatography, DEAE-Cellulose; D003065:Coenzyme A / Q000378:metabolism; D006168:Guinea Pigs; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008274:Magnesium / Q000378:metabolism; D008970:Molecular Weight; D009708:Nucleotidases / Q000302:isolation & purification* / Q000378:metabolism; D010724:Phosphoadenosine Phosphosulfate / Q000378:metabolism
chemical_list: D000596:Amino Acids; D010724:Phosphoadenosine Phosphosulfate; D000255:Adenosine Triphosphate; D009708:Nucleotidases; C053354:bisphosphoadenylate 3'-nucleotidase; D008274:Magnesium; D003065:Coenzyme A