title: Copper transport from Cu(I)-thionein into apo-caeruloplasmin mediated by activated leucocytes.
abstract: A study on the transfer of copper from Cu-thionein into apo-caeruloplasmin, using Cu-thionein that was previously oxidised by activated leucocytes, was performed. Cu(I)-thiolate oxidation was conveniently monitored by the progressive decline of the specific Cotton bands between 400 and 300 nm. The characteristic e.p.r. properties and NN-dimethyl-p-phenylenediamine oxidase activity indicated a successful formation of caeruloplasmin. Taking into account the simultaneous occurrence of leucocytes, apo-caeruloplasmin and Cu-thionein in blood plasma, such an interaction would favour a possible metabolic link between either copper protein.
mesh_terms: D000818:Animals; D001059:Apoproteins; D001692:Biological Transport; D002570:Ceruloplasmin / Q000378:metabolism*; D002942:Circular Dichroism; D003300:Copper / Q000097:blood*; D004578:Electron Spin Resonance Spectroscopy; D066298:In Vitro Techniques; D007962:Leukocytes / Q000378:metabolism*; D008668:Metallothionein / Q000097:blood*; D013552:Swine
chemical_list: D001059:Apoproteins; C006612:apoceruloplasmin; C007121:copper thionein; D003300:Copper; D008668:Metallothionein; D002570:Ceruloplasmin