Protein products synthesized by a cloned viral protease gene.
A region of the poliovirus genome coding for a cysteine protease was expressed in E. coli. Following separation in isoelectric focussing gels, four new proteins were identified and were shown to be products of the viral cDNA. Although a majority of the protein products is aggregated, the system offers the first practical basis for production of large quantities of a viral protease.
D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005814:Genes, Viral; D010447:Peptide Hydrolases / Q000096:biosynthesis / Q000378:metabolism*; D017955:Poliovirus / Q000201:enzymology* / Q000235:genetics; D012367:RNA, Viral / Q000032:analysis / Q000096:biosynthesis; D014764:Viral Proteins / Q000096:biosynthesis*
D012367:RNA, Viral; D014764:Viral Proteins; D010447:Peptide Hydrolases