Increased sensitivity of virus-infected cells to inhibitors of protein synthesis does not correlate with changes in plasma membrane permeability.
Semliki Forest virus-infected BHK cells or herpes simplex virus-infected Vero cells were incubated with the protein synthesis inhibitors hygromycin B and gougerotin. Infected cells take up no more [3H]hygromycin or [3H]gougerotin than do mock-infected cells, at a time p.i. at which either compound is more inhibitory to protein synthesis in infected, than in mock-infected cells. The concentrations of hygromycin and gougerotin required to inhibit protein synthesis in intact cells (irrespective of whether they are infected or not) are several orders of magnitude higher than those required in either permeabilized cells or in cell-free systems. Infected cells take up 86Rb+ at the same rate as mock-infected cells, their intracellular content of K+ is the same, and the activity of the Na+ pump is the same. It is concluded that the increased efficacy of hygromycin and gougerotin in virus-infected cells is a consequence of altered intracellular compartmentation and that increases in permeability of the plasma membrane, if any, are so small as to be undetectable by direct methods.
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001692:Biological Transport; D002414:Cations, Monovalent / Q000378:metabolism; D002451:Cell Compartmentation; D002463:Cell Membrane Permeability; D002934:Cinnamates; D006224:Cricetinae; D006561:Herpes Simplex / Q000503:physiopathology*; D006921:Hygromycin B / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology*; D014176:Protein Biosynthesis; D011741:Pyrimidine Nucleosides / Q000494:pharmacology; D012672:Semliki forest virus / Q000502:physiology; D018139:Simplexvirus / Q000502:physiology; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D014036:Togaviridae Infections / Q000503:physiopathology*; D014709:Vero Cells
D000900:Anti-Bacterial Agents; D002414:Cations, Monovalent; D002934:Cinnamates; D011741:Pyrimidine Nucleosides; D006921:Hygromycin B; C026273:hygromycin A; C004942:gougerotin; D000254:Sodium-Potassium-Exchanging ATPase