title: Two enzyme immunoassays for the detection of antibody to rodent coronaviruses.
abstract: Two enzyme immunoassays for detection of antibody to rodent coronaviruses were compared. Mouse hepatitis virus (MHV), strain JHM, antigen was in the form of formalin-fixed, infected 17 C1 1 cells. This antigen detected antibody to the homologous strain of MHV as well as to two heterologous MHV strains and a serologically related rat coronavirus, sialodacryodenititis virus. Antibody titers in assays using horseradish peroxidase (HRP)-conjugated or ureiase-conjugated anti-mouse IgG were substantially higher than in an indirect immunofluorescence assay. The ureiase assay was somewhat more sensitive than the HRP assay. MHV-JHM antigen was stable under a variety of storage conditions for at least two months.
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D000956:Antigens, Viral / Q000276:immunology; D003332:Coronaviridae / Q000276:immunology* / Q000302:isolation & purification; D005455:Fluorescent Antibody Technique; D006735:Horseradish Peroxidase; D007124:Immunoenzyme Techniques; D051379:Mice; D008815:Mice, Inbred Strains; D006517:Murine hepatitis virus / Q000276:immunology; D014510:Urease
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D006735:Horseradish Peroxidase; D014510:Urease