A luminescence Western blot with enhanced sensitivity for antibodies to human immunodeficiency virus.
A luminescence assay was adapted for detection of peroxidase-labelled secondary antibodies, which react specifically with antibodies to human immunodeficiency virus on Western blots. This method is about one hundred times more sensitive than either commercial enzyme-linked immunosorbent assay or the chromogenic peroxidase assay on Western blots and detects seroconversion earlier than any other technique currently available.
D000163:Acquired Immunodeficiency Syndrome / Q000175:diagnosis*; D000328:Adult; D000914:Antibodies, Viral / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D006678:HIV / Q000276:immunology*; D015483:HIV Antibodies; D006801:Humans; D007124:Immunoenzyme Techniques; D007527:Isoenzymes; D008163:Luminescent Measurements; D008297:Male; D009195:Peroxidase; D010544:Peroxidases; D011933:Reagent Kits, Diagnostic
D000914:Antibodies, Viral; D015483:HIV Antibodies; D007527:Isoenzymes; D011933:Reagent Kits, Diagnostic; D010544:Peroxidases; D009195:Peroxidase