title: Escherichia coli ribosomal protein S1 does not protect the 49-nucleotide 3' terminal cloacin fragment of 16 S rRNA from nuclease S1.
abstract: Footprinting of ribosomal protein S1 on the 49-nucleotide 3' terminal cloacin DF13 fragment of 16 S rRNA at physiological ionic strength, pH and temperature yielded no detectable protection of any nucleotides from subsequent attack by the single strand specific nuclease S1, even at large excesses of ribosomal protein S1.
mesh_terms: D001430:Bacteriocins / Q000235:genetics*; D001483:Base Sequence; D002989:Cloacin / Q000235:genetics*; D004720:Endonucleases; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D009690:Nucleic Acid Conformation; D009710:Nucleotide Mapping; D011485:Protein Binding; D012335:RNA, Ribosomal / Q000235:genetics / Q000378:metabolism*; D012269:Ribosomal Proteins / Q000378:metabolism*; D015719:Single-Strand Specific DNA and RNA Endonucleases
chemical_list: D001430:Bacteriocins; D012335:RNA, Ribosomal; D012269:Ribosomal Proteins; C015494:ribosomal protein S1; D002989:Cloacin; D004720:Endonucleases; D015719:Single-Strand Specific DNA and RNA Endonucleases