Separation of phosphoinositides and other phospholipids by two-dimensional thin-layer chromatography.
A simple, rapid, two-dimensional TLC system is presented which resolves the four phosphoinositide cycle phospholipids as well as all commonly encountered major and minor phospholipids. Ca2+-free lipid samples are loaded onto silica gel HL plates and developed first in 48:40:7:5 chloroform:methanol:water:concentrated ammonia, and then in 55:25:5 chloroform:methanol:formic acid. The method was applied successfully to human erythrocytes, human platelets, and BL/VL3 murine lymphoma cells.
D001792:Blood Platelets / Q000032:analysis; D002855:Chromatography, Thin Layer / Q000379:methods*; D004912:Erythrocytes / Q000032:analysis; D006801:Humans; D008223:Lymphoma / Q000032:analysis; D010716:Phosphatidylinositols / Q000032:analysis*; D010743:Phospholipids / Q000032:analysis*; D012997:Solvents
D010716:Phosphatidylinositols; D010743:Phospholipids; D012997:Solvents