title: Spin-trapping of sulfite radical anion, SO3-., by a water-soluble, nitroso-aromatic spin-trap.
abstract: Sulfite radical anion, SO3-., which is generated either by non-enzymatic reaction of hydrogen peroxide (H2O2-) with sulfite (SO3(2-)) or by the oxidation of bisulfite (HSO3) with Ce4+ ion, can be trapped with a water-soluble, nitroso-aromatic spin-trap, sodium 3,5-dibromo-4-nitrosobenzenesulfonate (DBNBS, 1), yielding an ESR spectrum with coupling constants [aN (1) = 12.9 G, aH (2) = 0.8 G] and a g-value of 2.0063. The SO3- radical adduct (spin adduct) was observed even in the presence of the very low concentration of H2O2 (1.21 X 10(-2) mumol).
mesh_terms: D001557:Benzenesulfonates / Q000494:pharmacology*; D002563:Cerium; D004578:Electron Spin Resonance Spectroscopy; D005609:Free Radicals; D006861:Hydrogen Peroxide; D009603:Nitroso Compounds / Q000494:pharmacology*; D013113:Spin Labels / Q000494:pharmacology*; D013447:Sulfites
chemical_list: D001557:Benzenesulfonates; D005609:Free Radicals; D009603:Nitroso Compounds; D013113:Spin Labels; D013447:Sulfites; D002563:Cerium; C048757:3,5-dibromo-4-nitrosobenzenesulfonate; D006861:Hydrogen Peroxide