In vitro packaging into phage T4 particles and specific recircularization of phage lambda DNAs.
Concatemeric phage lambda imm434 DNA packaged in vitro into phage T4 particles produced plaques on a selective host. Moreover, lambda DNA containing a pBR322 derivative flanked by the lambda attL and attR sites could be specifically recircularized by excisive lambda recombination to yield the pBR322 derivative. A host deficient in generalized recombination and containing a defective lambda c Its prophage which provided Int and Xis proteins was the recipient for this plasmid derivative carried by T4. Such a T4-lambda hybrid may potentially allow almost one T4 headful of donor DNA (166 kb) to be packaged and recircularized.
D010582:Bacteriophage lambda / Q000235:genetics*; D004262:DNA Restriction Enzymes; D004270:DNA, Circular / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D010957:Plasmids; D013604:T-Phages / Q000235:genetics*
D004270:DNA, Circular; D004279:DNA, Viral; D004262:DNA Restriction Enzymes