Chimeric antibody with human constant regions and mouse variable regions directed against carcinoma-associated antigen 17-1A.
We have cloned the genomic DNA fragments encoding the heavy and light chain variable regions of monoclonal antibody 17-1A, and we have inserted them into mammalian expression vectors containing genomic DNA segments encoding human gamma 3 and kappa constant regions. The transfer of these expression vectors containing mouse-human chimeric immunoglobulin genes into Sp2/0 mouse myeloma cells resulted in the production of functional IgG that retained the specific binding to the surface antigen 17-1A expressed on colorectal carcinoma cells.
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D000917:Antibody Formation; D000951:Antigens, Neoplasm / Q000276:immunology*; D001483:Base Sequence; D002678:Chimera; D004247:DNA / Q000378:metabolism; D004262:DNA Restriction Enzymes; D006801:Humans; D007127:Immunoglobulin Constant Regions / Q000235:genetics* / Q000276:immunology; D007135:Immunoglobulin Variable Region / Q000235:genetics* / Q000276:immunology; D007136:Immunoglobulins / Q000235:genetics*; D051379:Mice; D009693:Nucleic Acid Hybridization
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D007127:Immunoglobulin Constant Regions; D007135:Immunoglobulin Variable Region; D007136:Immunoglobulins; C050928:carcinoma-associated antigen 17-1A; D004247:DNA; D004262:DNA Restriction Enzymes