Contribution of exogenous progesterone to human adrenal cortisol synthesis in vitro: a comparison of early gestation fetal and adult tissues.
The ability of human adult adrenal to utilize progesterone (P4) for cortisol (F) synthesis in vitro has been compared with that of fetal adrenal tissue. Explant cultures were studied for 6 days using Ham's F10 medium supplemented with 10% fetal bovine serum (FBS), with or without added P4 as substrate. Short-term (4h) incubations of fresh tissue minces were carried out in Ham's F10, with or without P4, in the absence of FBS. In contrast with the fetal gland, F production by cultured adult tissue was unaffected by addition of P4. During short-term incubations, P4 increased F production 150-fold in the fetal tissue as compared to 2- to 4-fold in the adult. ACTH had no acute effect on the P4 to F conversion in either tissue. These results demonstrate that the fetal adrenal exhibits a greater ability to utilize P4 for F production than the adult adrenal.
D000293:Adolescent; D000302:Adrenal Cortex / Q000196:embryology / Q000378:metabolism*; D000305:Adrenal Cortex Hormones / Q000096:biosynthesis; D000324:Adrenocorticotropic Hormone / Q000494:pharmacology; D000328:Adult; D003687:Dehydroepiandrosterone / Q000096:biosynthesis; D005260:Female; D006801:Humans; D006854:Hydrocortisone / Q000096:biosynthesis*; D006908:Hydroxyprogesterones / Q000096:biosynthesis; D008297:Male; D009924:Organ Culture Techniques; D011374:Progesterone / Q000378:metabolism*
D000305:Adrenal Cortex Hormones; D006908:Hydroxyprogesterones; D003687:Dehydroepiandrosterone; D011374:Progesterone; D000324:Adrenocorticotropic Hormone; D006854:Hydrocortisone