title: Isolation of collagen binding proteins from embryonic chicken corneal epithelial cells.
abstract: In the present paper, we report the isolation and characterization of embryonic corneal membrane glycoproteins that demonstrate specific affinity for collagen. Two collagen binding proteins have been isolated: a novel 70-kDa protein and a 47-kDa protein which is apparently similar to that reported by Kurkinen et al. (Kurkinen, M., Taylor, A., Garrels, J. I., and Hogan, B. (1984) J. Biol. Chem. 259, 5915-5922). Both proteins label metabolically with [35S]methionine and [3H] glucosamine. 125I iodination of cell surface proteins revealed that the two collagen binding proteins are expressed on the epithelial cell surface. The 70-kDa protein appears to be an integral membrane protein, whereas the 47-kDa protein can be removed from membranes by alkali treatment. The isolated proteins exhibit binding to native type IV collagen as well as heat-denatured type I collagen. It seems likely that we have isolated, at least in part, the cell surface receptor or receptor complex that binds collagen to the basal surface of epithelia.
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000302:isolation & purification* / Q000378:metabolism; D002462:Cell Membrane / Q000032:analysis; D002642:Chick Embryo; D002846:Chromatography, Affinity; D003094:Collagen / Q000378:metabolism; D003315:Cornea / Q000032:analysis* / Q000196:embryology; D004591:Electrophoresis, Polyacrylamide Gel; D004848:Epithelium / Q000032:analysis; D005109:Extracellular Matrix / Q000378:metabolism; D005944:Glucosamine / Q000378:metabolism; D008081:Liposomes / Q000378:metabolism; D008565:Membrane Proteins / Q000302:isolation & purification; D008715:Methionine / Q000378:metabolism; D008970:Molecular Weight; D011956:Receptors, Cell Surface / Q000302:isolation & purification* / Q000378:metabolism; D038981:Receptors, Collagen
chemical_list: D002352:Carrier Proteins; D008081:Liposomes; D008565:Membrane Proteins; D011956:Receptors, Cell Surface; D038981:Receptors, Collagen; D003094:Collagen; D008715:Methionine; D005944:Glucosamine