title: Alkaline protease deficiency in the cr-1 (crisp) mutant of Neurospora crassa.
abstract: The adenyl cyclase deficient cr-1 mutant of Neurospora crassa grew poorly in bovine serum albumin as an alternative and only source of either sulfur, nitrogen or carbon. The low growth of the cr-1 mutant in protein was correlated with limited secretion of extracellular alkaline protease. The defect was specific for the cr-1 mutant and was suppressed by exogenous cyclic AMP. Cyclic AMP relieved protease deficiency under carbon, nitrogen or sulfur limiting conditions to unequal extents. Protease stimulation was greatest under carbon-limited conditions, but the resulting growth was least. Most of the cyclic AMP-mediated increase of alkaline protease was extracellular.
mesh_terms: D000262:Adenylyl Cyclases / Q000172:deficiency*; D000242:Cyclic AMP / Q000494:pharmacology*; D009491:Neurospora / Q000201:enzymology*; D009492:Neurospora crassa / Q000201:enzymology* / Q000235:genetics; D010447:Peptide Hydrolases / Q000378:metabolism*; D010641:Phenotype
chemical_list: D000242:Cyclic AMP; D010447:Peptide Hydrolases; D000262:Adenylyl Cyclases