Interaction of PGF2 alpha with prolactin on the release of cyclic AMP and progesterone from the isolated perfused ovary of the pregnant mare serum gonadotropin-treated rat.
A newly developed model for perfusion of the isolated rat ovary was employed to study the interactions of Prl with PGF2 alpha in respect to the effects of LH on cAMP formation and progesterone production in the 5 day old corpus luteum of the PMSG-treated rat. An inhibitory effect of PGF2 alpha on both basal and LH stimulated progesterone secretion was found. This block also involved inhibition of the ovarian cAMP release which was not associated with a reduction of the flow of the medium to the ovary. When Prl was present in the medium the PGF2 alpha block of LH-induced cAMP release was reversed. However, Prl failed to restore block of LH stimulation of progesterone secretion in 4 out of 9 experiments, indicating an additional site of action of PGF2 alpha distal to the cAMP in these experiments.
D000818:Animals; D000242:Cyclic AMP / Q000096:biosynthesis*; D015237:Dinoprost; D004347:Drug Interactions; D005260:Female; D006064:Gonadotropins, Equine / Q000494:pharmacology*; D007986:Luteinizing Hormone / Q000494:pharmacology; D010053:Ovary / Q000378:metabolism*; D010477:Perfusion; D011374:Progesterone / Q000096:biosynthesis*; D011388:Prolactin / Q000494:pharmacology*; D011460:Prostaglandins F / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains
D006064:Gonadotropins, Equine; D011460:Prostaglandins F; D011374:Progesterone; D011388:Prolactin; D007986:Luteinizing Hormone; D015237:Dinoprost; D000242:Cyclic AMP