Butyrate-induced reversal of herpes simplex virus restriction in neuroblastoma cells.
The synthesis of herpes simplex virus (HSV) in mouse neuroblastoma cells (NB, clone 41A3) is restricted. There was a disappearance of infectious virus upon serial passage of infected cells. NB cells treated with sodium-n-butyrate for 24 hr before infection synthesized 200-2000 times more HSV than untreated cells. Infectious center assays demonstrated that the number of cells capable of producing HSV was increased as a result of butyrate pretreatment. Although host protein synthesis was inhibited by HSV infection, viral-induced protein and DNA syntheses were not detected in the absence of butyrate. Cycloheximide blocked the induction of permissiveness by butyrate suggesting that a protein(s) was responsible for allowing HSV synthesis in NB cells. Regulatable host factors involved in HSV replication in neural cells can be studied in the system described.
D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D002087:Butyrates / Q000494:pharmacology*; D020148:Butyric Acid; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D004279:DNA, Viral / Q000096:biosynthesis; D005227:Fatty Acids / Q000494:pharmacology; D051379:Mice; D009447:Neuroblastoma / Q000382:microbiology*; D009696:Nucleic Acids / Q000096:biosynthesis; D014176:Protein Biosynthesis; D018139:Simplexvirus / Q000254:growth & development*; D013329:Structure-Activity Relationship; D013997:Time Factors; D014779:Virus Replication / Q000187:drug effects*
D002087:Butyrates; D004279:DNA, Viral; D005227:Fatty Acids; D009696:Nucleic Acids; D020148:Butyric Acid; D003994:Bucladesine