title: The localization of cholinephosphotransferase in the outer membrane of guinea-pig lung mitochondria.
abstract: The activity of cholinephosphotransferase was measured in the subcellular fractions of guinea-pig lung. The specific activity of the enzyme was highest in a fraction, intermediate in density between mitochondria and microsomes. Similar subcellular distribution patterns were observed for both cholinephosphotransferase and rotenone-insensitive NADH-cytochrome c reductase, an enzyme associated with the outer membrane of mitochondria and endoplasmic reticulum, suggesting that cholinephosphotransferase may be localized in both of these organelles. The distribution of cholinephosphotransferase activity in the subfractions of mitochondria and the intermediate fractions recovered by linear density gradient paralleled that of the mitochondrial outer membrane marker enzyme, monoamine oxidase. RNA content of a subfraction enriched in cholinephosphotransferase and monoamine oxidase was not typical to that of either rough or smooth endoplasmic reticulum. The results of this study suggest that in guinea-pig lung, cholinephosphotransferase is localized in both the outer membrane of mitochondria, and the endoplasmic reticulum.
mesh_terms: D000818:Animals; D002458:Cell Fractionation / Q000379:methods; D002499:Centrifugation, Density Gradient / Q000379:methods; D002798:Diacylglycerol Cholinephosphotransferase / Q000378:metabolism*; D005260:Female; D006168:Guinea Pigs; D007425:Intracellular Membranes / Q000201:enzymology*; D008168:Lung / Q000201:enzymology*; D008928:Mitochondria / Q000201:enzymology*; D010770:Phosphotransferases / Q000378:metabolism*; D013347:Subcellular Fractions / Q000201:enzymology
chemical_list: D010770:Phosphotransferases; D002798:Diacylglycerol Cholinephosphotransferase