Functional comparison of bone marrow-derived macrophages obtained by cultivation in serum-free or serum-supplemented medium.
Bone marrow-derived macrophages obtained by cultivation in a serum-free or in a serum-supplemented medium were compared in terms of the activation of the respiratory burst and the activation of tumor cytotoxicity. Serum-free-cultured macrophages responded to interferon-gamma (IFN-gamma) and to lipopolysaccharide (LPS) by an enhancement of the respiratory burst. Macrophages obtained in a serum-supplemented medium are characterized by a diminished capacity to release O2-. These cells did not respond to IFN-gamma unless the stimulation was performed in a serum-containing medium. In terms of activation of tumor cell cytotoxicity, serum-supplemented macrophage cultures seem to be primed by unknown serum constituents because they only need one signal (IFN-gamma or LPS) to become fully cytotoxic. Serum-free cultivated macrophages can be rendered cytotoxic only after exposure to combinations of IFN-gamma and LPS.
D000818:Animals; D001798:Blood Proteins / Q000502:physiology*; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D003470:Culture Media / Q000502:physiology; D003602:Cytotoxicity, Immunologic; D005260:Female; D007371:Interferon-gamma / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008163:Luminescent Measurements; D008262:Macrophage Activation; D008264:Macrophages / Q000187:drug effects / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL / Q000145:classification; D009374:Neoplasms, Experimental / Q000276:immunology; D013481:Superoxides / Q000032:analysis / Q000378:metabolism
D001798:Blood Proteins; D003470:Culture Media; D008070:Lipopolysaccharides; D013481:Superoxides; D007371:Interferon-gamma