Evidence for endothelium-dependent vasodilation of resistance vessels by acetylcholine.
In the perfused mesenteric arterial vasculature of the rabbit, vasodilation by acetylcholine (ACh) was almost completely blocked after a 15-min perfusion of the vasculature with 0.2% collagenase, an enzyme capable of removing endothelial cells. In the perfused mesenteric arterial vasculature of the rat, vasodilation by ACh was markedly, though not completely, inhibited by hemoglobin (10 microM), an agent which can inactivate endothelium-derived relaxing factor (EDRF). These results suggest that a major component of vasodilation of mesenteric resistance vessels in rabbit and rat by ACh is mediated by EDRF.
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D001808:Blood Vessels / Q000187:drug effects / Q000502:physiology*; D004727:Endothelium / Q000187:drug effects / Q000502:physiology; D006454:Hemoglobins / Q000502:physiology; D003012:Microbial Collagenase / Q000494:pharmacology; D009569:Nitric Oxide; D011817:Rabbits; D051381:Rats; D013152:Splanchnic Circulation / Q000187:drug effects; D014655:Vascular Resistance; D014664:Vasodilation; D014665:Vasodilator Agents / Q000037:antagonists & inhibitors / Q000502:physiology
D006454:Hemoglobins; D014665:Vasodilator Agents; D009569:Nitric Oxide; D003012:Microbial Collagenase; D000109:Acetylcholine