Studies on immunoassays of peptide factors. V. Synthesis of cholecystokinin-58-[1-11]/iso-1-cytochrome c conjugate.
In previous studies on model compounds we have found that the maleimide function is sufficiently stable under the conditions of peptide synthesis to allow its incorporation at preselected positions of a peptide chain in earlier steps of the synthetic route. Taking advantage of this observation the N-terminal undecapeptide of canine cholecystokinin-58 containing at its N-terminus the maleimide group became accessible in high yields as chromatographically homogenous and analytically well characterized compound. Via the incorporated anchor group the undecapeptide was linked selectively at its N-terminus to the cysteine residue 107 of iso-1-cytochrome c to yield a well characterized conjugate of 1:1 stoichiometry for immunization experiments.
D002766:Cholecystokinin / Q000032:analysis / Q000138:chemical synthesis*; D002850:Chromatography, Gel; D003574:Cytochrome c Group / Q000032:analysis / Q000138:chemical synthesis*; D045304:Cytochromes c; D007118:Immunoassay; D046911:Macromolecular Substances; D010446:Peptide Fragments / Q000032:analysis; D029701:Saccharomyces cerevisiae Proteins
C014059:CYC1 protein, S cerevisiae; D003574:Cytochrome c Group; D046911:Macromolecular Substances; D010446:Peptide Fragments; D029701:Saccharomyces cerevisiae Proteins; C038823:cholecystokinin 58; D045304:Cytochromes c; D002766:Cholecystokinin