cDNA probes of individual genes of human rotavirus distinguish viral subgroups and serotypes.
The use of cDNA probes for detection of rotaviruses has been investigated using plasmids containing inserts specific for each of the eleven genes of human rotavirus strain Wa. In a dot-blot detection system in which radioactive DNA probes were hybridized to viral RNA extracted from cultivatable rotavirus strains, cDNAs of genes 7, 8, 10 and 11, were found to be the most reliable probes for detecting a range of rotavirus strains. Unexpectedly, rotaviruses could be distinguished with respect to subgroup and subtype specificities when cDNAs of genes 6 and 9, which encode the immunologically relevant proteins VP6 (group-specific antigen) and VP7 (type-specific antigen), were used as probe, even though the nucleic acid sequences of these genes are known to have a high degree of sequence homology.
D002675:Child, Preschool; D004247:DNA / Q000235:genetics; D004274:DNA, Recombinant; D004279:DNA, Viral; D003968:Diarrhea, Infantile / Q000175:diagnosis* / Q000209:etiology / Q000382:microbiology; D005814:Genes, Viral; D006801:Humans; D007223:Infant; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000032:analysis; D012401:Rotavirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012400:Rotavirus Infections / Q000175:diagnosis* / Q000382:microbiology; D012689:Sequence Homology, Nucleic Acid
D004274:DNA, Recombinant; D004279:DNA, Viral; D012367:RNA, Viral; D004247:DNA