Sidedness of ceramide-phosphoethanolamine synthesis on rat liver and brain microsomal membranes.
Phosphatidylethanolamine:ceramide-ethanolamine-phosphotransferase catalyzes the synthesis of ceramide-phosphoethanolamine, a sphingomyelin analogue. Its localization was studied in rat liver and brain microsomes. After testing the integrity and the sidedness of microsomal vesicles, trypsin treatment of intact or deoxycholate-disrupted microsomes made it possible to conclude that both the transferase and the ceramide-phosphoethanolamine are located in the cisternal leaflet of the membrane bilayer. Using trinitrobenzenesulfonic acid as a probe, no trace of newly synthesized ceramide-phosphoethanolamine was detectable on the cytoplasmic side of the microsomes.
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002518:Ceramides / Q000096:biosynthesis*; D004721:Endoplasmic Reticulum / Q000378:metabolism; D066298:In Vitro Techniques; D007425:Intracellular Membranes / Q000378:metabolism; D008861:Microsomes / Q000378:metabolism; D008862:Microsomes, Liver / Q000378:metabolism*; D010714:Phosphatidylethanolamines / Q000096:biosynthesis*; D010770:Phosphotransferases / Q000378:metabolism; D051381:Rats; D013109:Sphingomyelins / Q000378:metabolism; D017855:Transferases (Other Substituted Phosphate Groups)
D002518:Ceramides; D010714:Phosphatidylethanolamines; D013109:Sphingomyelins; D010770:Phosphotransferases; C019856:CDP-ethanolamine-ceramide ethanolaminephosphotransferase; D017855:Transferases (Other Substituted Phosphate Groups)