title: Biosynthesis of cholesterol in the yeast mutant erg6.
abstract: A mutant (erg6) of Saccharomyces cerevisiae defective in S-adenosylmethionine: delta 24-sterol-C-methyl transferase (EC2.1.1.41) which normally produces cholesta-5,7,24-trienol and cholesta-5,7,22,24-tetraenol as the major sterols (total 4,4-desmethyl sterol content-8.3 fg/cell) was shown to synthesize trace levels of cholesterol (0.08 fg/cell). The identity of cholesterol was established by co-chromatography in TLC, GLC and HPLC with an authentic sample, mass spectroscopy and after an incubation with [1-14C]acetate by isotopic dilution and recrystallization of the radiochemically purified material to constant specific activity.
mesh_terms: D002777:Cholestanols / Q000302:isolation & purification; D002784:Cholesterol / Q000096:biosynthesis* / Q000302:isolation & purification; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D008401:Gas Chromatography-Mass Spectrometry; D009154:Mutation; D012436:S-Adenosylmethionine / Q000172:deficiency; D012441:Saccharomyces cerevisiae / Q000032:analysis / Q000235:genetics / Q000378:metabolism*
chemical_list: D002777:Cholestanols; D012436:S-Adenosylmethionine; D002784:Cholesterol