title: Sensitive in situ hybridisation technique using biotin-streptavidin-polyalkaline phosphatase complex.
abstract: A sensitive in situ hybridisation technique, using a biotin-streptavidin-polyalkaline phosphatase complex detection system, was successfully applied to smears of fresh cultured cells, frozen sections, and formalin fixed paraffin processed tissue: the procedure was successful for DNA-DNA hybridizations using a variety of DNA probes. The detection method is rapid, reliable, and economical producing a purplish-blue precipitate at the site of hybridisation and clearly visible by low power light microscopy.
mesh_terms: D000469:Alkaline Phosphatase; D001426:Bacterial Proteins; D001710:Biotin; D004247:DNA / Q000032:analysis*; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D008722:Methods; D009693:Nucleic Acid Hybridization; D027383:Papillomaviridae; D010957:Plasmids; D019809:Streptavidin; D014998:Y Chromosome
chemical_list: D001426:Bacterial Proteins; D001710:Biotin; D004247:DNA; D019809:Streptavidin; D000469:Alkaline Phosphatase