Structure and expression in Escherichia coli of canine interferon-alpha genes.
Using a human interferon-alpha (IFN-alpha) cDNA probe, several recombinant phages containing type I IFN genes were isolated from a canine genomic library. One of these phages contains two complete CaIFN-alpha genes with identical coding sequences, and a second one a slightly different IFN-alpha gene. The IFN-alpha protein sequences contain six cysteine residues as well as two or three potential N-glycosylation sites. Expression of mature CaIFN-alpha 1 in E. coli results in antiviral activity on dog cells. Genomic analysis using an equine IFN-omega probe and DNA sequencing suggests the deletion of IFN-omega genes from canine genome.
D000595:Amino Acid Sequence; D000818:Animals; D010582:Bacteriophage lambda / Q000235:genetics; D001483:Base Sequence; D004247:DNA / Q000378:metabolism; D004262:DNA Restriction Enzymes; D004285:Dogs; D004926:Escherichia coli / Q000235:genetics*; D005796:Genes; D006801:Humans; D007370:Interferon Type I / Q000235:genetics*; D008099:Liver / Q000378:metabolism; D010957:Plasmids
D007370:Interferon Type I; D004247:DNA; D004262:DNA Restriction Enzymes