Hydroperoxide-induced radical production in liver mitochondria.
When isolated rat liver mitochondria are treated with tert-butyl hydroperoxide in the presence of the spin trap 5,5-dimethyl-1-pyrroline-N-oxide, a six-line ESR signal is observed with parameters characteristic of a carbon-centered radical. The radical is shown to be CH3. using 2-methyl-2-nitrosopropane as the spin trap. Inhibition of radical production by EDTA and N-ethylmaleimide provides evidence for participation by metals and reduced sulfhydryl groups in the radical-generating reaction. It is proposed that radicals are formed through the reaction between a reducing agent, a metal and the hydroperoxide.
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D003497:Cyclic N-Oxides; D004492:Edetic Acid / Q000494:pharmacology; D004578:Electron Spin Resonance Spectroscopy; D005033:Ethylmaleimide / Q000494:pharmacology; D005609:Free Radicals; D008297:Male; D008697:Methane / Q000031:analogs & derivatives* / Q000378:metabolism; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism*; D009603:Nitroso Compounds; D010100:Oxygen / Q000494:pharmacology; D010545:Peroxides / Q000494:pharmacology*; D051381:Rats; D013113:Spin Labels; D020122:tert-Butylhydroperoxide
D003497:Cyclic N-Oxides; D005609:Free Radicals; D009603:Nitroso Compounds; D010545:Peroxides; D013113:Spin Labels; C051224:methyl radical; C017245:5,5-dimethyl-1-pyrroline-1-oxide; D020122:tert-Butylhydroperoxide; D004492:Edetic Acid; C034526:tert-nitrosobutane; D005033:Ethylmaleimide; D008697:Methane; D010100:Oxygen; D002118:Calcium