Is efficiency of suppressor tRNAs controlled at the level of ribosomal proofreading in vivo?
Ribosomal rpsD mutations did not stimulate nonsense suppressor tRNAs in a general manner according to their increased ribosomal ambiguity and decreased proofreading efficiency. Streptomycin, which stimulates error production by blocking proofreading in vitro, did not increase efficiency of suppressor tRNAs in strains with normal or streptomycin-resistant (rpsL) ribosomes. It did so only in combination with one rpsL mutation which is associated with streptomycin pseudodependence.
D003062:Codon / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics*; D029968:Escherichia coli Proteins; D009154:Mutation; D014176:Protein Biosynthesis; D012343:RNA, Transfer / Q000235:genetics*; D000091243:Ribosomal Protein S9; D012270:Ribosomes / Q000187:drug effects* / Q000378:metabolism; D013307:Streptomycin / Q000494:pharmacology*; D013489:Suppression, Genetic / Q000187:drug effects
D003062:Codon; D029968:Escherichia coli Proteins; D000091243:Ribosomal Protein S9; C000717190:RpsI protein, E coli; D012343:RNA, Transfer; D013307:Streptomycin