Detection of bovine herpesvirus-1 nucleic acid sequences, using a dot-blot hybridization procedure.
A sensitive and specific procedure for the detection of Argentine isolates of bovine herpesvirus-1 was developed. The procedure was based on a dot-blot, nucleic acid hybridization, using 32P, nick-translated, plasmidic probes. The probes contained cloned Bam H1 restriction fragments in the left half of the viral genome. The detection limit of the procedure was 10 pg of viral DNA.
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000032:analysis*; D015245:Deoxyribonuclease BamHI; D015246:Deoxyribonuclease EcoRI; D007242:Herpesvirus 1, Bovine / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D010957:Plasmids
D004279:DNA, Viral; D004262:DNA Restriction Enzymes; D015245:Deoxyribonuclease BamHI; D015246:Deoxyribonuclease EcoRI