title: Synthetic peptide substrates as models to study a pro-ocytocin/neurophysin converting enzyme.
abstract: The selectivity and mechanism of processing at paired basic amino acids in hormone precursors was studied on several analogues of the (1-20)-aminoterminal domain of the ocytocin/neurophysin precursor in a cleavage assay by an endoprotease partially purified from bovine pituitary secretory granules. Peptide analogues with amino acid substitutions in, and around, the basic doublet were synthesized and used as substrates. The data obtained demonstrate the strict requirement of the processing enzyme for basic amino acids in tandem within a possibly preferred conformation which may be highly conserved in the aminoterminal domain of this hormone precursor.
mesh_terms: D000818:Animals; D001923:Brain Chemistry; D002162:Camelus; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D010450:Endopeptidases / Q000378:metabolism*; D013058:Mass Spectrometry; D009481:Neurophysins / Q000378:metabolism*; D010121:Oxytocin / Q000378:metabolism*; D011487:Protein Conformation; D011498:Protein Precursors / Q000378:metabolism*; D013329:Structure-Activity Relationship
chemical_list: D009481:Neurophysins; D011498:Protein Precursors; D010121:Oxytocin; D010450:Endopeptidases; C056535:pro-ocytocin-neurophysin convertase