The electronic and magnetic properties of rubredoxin: a low-temperature magnetic circular dichroism study.
Oxidized rubredoxin from Clostridium pasteurianum has been investigated by magnetic circular dichroism (MCD) spectroscopy over the temperature range 1.5 to 150 K and at magnetic fields between 0 and 4.5 tesla. The results show that studies of the temperature and field dependence of MCD transitions afford insight into the polarization of electronic transitions for ground states with large g-value anisotropy, in addition to estimates of ground-state g values and zero-field splitting parameters. In agreement with the assignment made by Eaton and Lovenberg (Eaton, W.A. and Lovenberg, W. (1973) in Iron-Sulfur Proteins, Vol. II (Lovenberg, W., ed.), pp. 131-162, Academic Press, New York), the ultraviolet-visible spectrum of oxidized rubredoxin is assigned to two S----Fe(III) charge transfer transitions (both 6A1----6T2 under tetrahedral symmetry), each spanning a range of 650-430 nm and 430-330 nm, respectively. The observed splitting in each of these transitions is attributed to a predominant axial distortion in the excited state resulting in effective D2d symmetry.
D002942:Circular Dichroism; D003013:Clostridium / Q000032:analysis; D004578:Electron Spin Resonance Spectroscopy; D005288:Ferredoxins; D008280:Magnetics; D010084:Oxidation-Reduction; D012416:Rubredoxins; D013053:Spectrophotometry
D005288:Ferredoxins; D012416:Rubredoxins