title: Virulence of bluetongue virus for British sheep.
abstract: A South African isolate of bluetongue virus type 3 was inoculated intradermally into three different breeds of British sheep under conditions designed to test its virulence in animals under stress. All animals inoculated developed a pyrexia and viraemia followed by clinical evidence of bluetongue disease. Marked alterations in serum enzyme levels, in particular of creatine phosphokinase, lactate dehydrogenase and aldolase occurred in the more severely affected animals. Nine out of the 12 inoculated animals subsequently died. No major differences in response could be detected in the different breeds of sheep nor in the stressed compared with the unstressed groups. The virulence of this bluetongue virus isolate was thereby confirmed and its potential risk to the British sheep industry. Consequently, stringent import regulations must be maintained to prevent its entry into Britain.
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D001777:Blood Coagulation; D001819:Bluetongue / Q000097:blood / Q000276:immunology / Q000382:microbiology*; D001820:Bluetongue virus / Q000276:immunology / Q000472:pathogenicity*; D003402:Creatine Kinase / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay; D005634:Fructose-Bisphosphate Aldolase / Q000097:blood; D005779:Immunodiffusion; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008297:Male; D009500:Neutralization Tests; D012087:Reoviridae / Q000472:pathogenicity*; D012756:Sheep; D013312:Stress, Physiological / Q000662:veterinary; D014766:Viremia / Q000662:veterinary; D014774:Virulence
chemical_list: D000914:Antibodies, Viral; D007770:L-Lactate Dehydrogenase; D003402:Creatine Kinase; D005634:Fructose-Bisphosphate Aldolase