[Adaptation of hepatitis A virus strain (JaM-55) originally pathogenic for monkeys to a cell culture].
The results of adaptation of hepatitis A viral strain JaM-55 to the culture of embryo kidney cells FRhk-4 from macaque Rhesus are presented. The viral strain was isolated from a M. fascicularis suffering from spontaneous hepatitis. Before inoculating the cell culture the virus was passaged twice in the M. arctoides capable of reproducing hepatitis. FRhk-4 cell line inoculation by the monkey liver extract, containing the strain HAV-YaM-55, resulted in isolation of single viral particles of hepatitis A in the preparations obtained at the first 3 passages by the 28-31 day of cultivation. Beginning from the fourth passage the abrupt increase in the number of viral particles and hepatitis A antigen was registered. There were no traces of cytopathogenic effect at any level of viral passages in the inoculated cell culture. The adapted virus contains hepatitis A viral RNA identified by spot hybridization with the cloned cDNA of hepatitis A virus.
D000220:Adaptation, Biological; D000818:Animals; D006507:Hepatovirus / Q000472:pathogenicity / Q000502:physiology*; D006801:Humans; D008099:Liver / Q000382:microbiology; D008251:Macaca / Q000382:microbiology*; D014776:Virus Cultivation; D014779:Virus Replication
Adaptatsiia shtamma virusa gepatita A (IaM-55), iskhodno patogennogo dlia obez'ian, k kletochnoĭ kul'ture.