[Alpha interferon interaction with opiate receptors in the rat brain].
The preferential interactions of alpha-interferon (alpha-IFN) with delta and mu opiate receptors were studied. alpha-IFN (specific antiviral activity 2 X 10(3) U/mg protein) was shown to inhibit in the competitive manner 3H-naloxone and 3H-D-ala2, D-leu5-enkephalin (3H-DADL) specific binding to opiate receptor subpopulations. alpha-IFN was much more effective in decreasing 3H-DADL than 3H-naloxone binding in opiate receptors: K1 values averaged 160 +/- 30 and 1150 +/- 80 U/ml, respectively. IFN effective concentrations inhibiting 50% of 3H-naloxone opiate receptor binding in the absence or presence of 100 mmol/l NaCl were similar, and the "sodium shift" value was equal to 1. The independence of alpha-IFN activity of the presence of NA+ cations suggests the antagonist character of alpha-IFN interaction with opiate receptors. Thus, alpha-IFN employed appears to be an alpha-selective ligand displaying the in vitro properties of "pure" morphine antagonists.
D000818:Animals; D001414:Bacitracin / Q000378:metabolism; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D004347:Drug Interactions; D004743:Enkephalin, Leucine / Q000031:analogs & derivatives / Q000378:metabolism; D016308:Enkephalin, Leucine-2-Alanine; D007370:Interferon Type I / Q000378:metabolism / Q000494:pharmacology*; D009270:Naloxone / Q000378:metabolism; D011485:Protein Binding / Q000187:drug effects; D051381:Rats; D011957:Receptors, Opioid / Q000187:drug effects* / Q000378:metabolism; D014316:Tritium
D007370:Interferon Type I; D011957:Receptors, Opioid; D014316:Tritium; D001414:Bacitracin; D009270:Naloxone; D004743:Enkephalin, Leucine; D016308:Enkephalin, Leucine-2-Alanine
Vzaimodeĭstvie al'fa-interferona s opiatnymi retseptorami golovnogo mozga krys.