Comparison between indirect immunofluorescence and microneutralization for detection of antibodies to polioviruses.
Indirect immunofluorescence and microneutralization methods for the detection of antibodies to poliovirus serotypes 1, 2, and 3 were compared. Of the 41 sera tested for poliovirus type 1 antibody, 40% were in complete agreement, 55% differed by one dilution, and 5% differed by two dilutions. For poliovirus type 2, 37 sera were tested; 56% completely agreed, and 44% differed by only one dilution. For poliovirus type 3, complete agreement occurred in 59% of 33 sera, while 41% differed by one dilution. No false-negative results were obtained. These findings suggest that indirect immunofluorescence for poliovirus types 1, 2, and 3 is as sensitive as the microneutralization method and could represent a less cumbersome alternative.
D000328:Adult; D000914:Antibodies, Viral / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D006801:Humans; D009500:Neutralization Tests; D017955:Poliovirus / Q000145:classification / Q000276:immunology*; D011054:Poliovirus Vaccine, Inactivated / Q000276:immunology; D012703:Serotyping
D000914:Antibodies, Viral; D011054:Poliovirus Vaccine, Inactivated