Expression of an activation antigen, Mo3e, associated with the cellular response to migration inhibitory factor by HL-60 promyelocytes undergoing monocyte-macrophage differentiation.
HL-60 promyelocytic cells acquire the surface expression of the Mo3e antigenic determinant after exposure to PMA or compounds that raise intracellular concentrations of cyclic AMP (dibutyryl cyclic AMP or a combination of cholera toxin and IBMX). The expression of Mo3e by these stimulated HL-60 cells coincides with the development of features of monocyte-macrophage differentiation (characteristic morphology, nonspecific esterase activity, and respiratory burst activity). During in vitro monocyte-macrophage differentiation, HL-60 cells become responsive to migration inhibitory factor (MIF); the MIF responsiveness of differentiated HL-60 cells is blocked by anti-Mo3e monoclonal antibody. These findings further support the relationship between the expression of Mo3e and the cellular response to MIF.
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000276:immunology*; D002454:Cell Differentiation; D002460:Cell Line; D000242:Cyclic AMP / Q000502:physiology; D005434:Flow Cytometry; D006801:Humans; D007163:Immunosorbent Techniques; D008262:Macrophage Activation; D008263:Macrophage Migration-Inhibitory Factors / Q000494:pharmacology*; D008264:Macrophages / Q000166:cytology / Q000276:immunology*; D008970:Molecular Weight; D009000:Monocytes / Q000166:cytology / Q000276:immunology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D008263:Macrophage Migration-Inhibitory Factors; D000242:Cyclic AMP; D013755:Tetradecanoylphorbol Acetate