Random integration of SV40 in SV40-transformed, immortalized human fibroblasts.
We have studied the relationship between immortalization of SV40-transformed human embryonic fibroblasts and their SV40 integration sites. From several independently transformed cell pools, we have isolated clones which do not harbor unintegrated SV40 DNA. We have analysed whole-cell DNA from these clones, using the Southern blot method. Our results suggest that no specific integration sites in the cellular genome exist which are a prerequisite for the immortalization process. Although some integration sites were found to be predominant in pre-crisis clones, they could not be detected in the post-crisis clones. This suggests that none of these predominating sites is selected for during the crisis period.
D002460:Cell Line; D002472:Cell Transformation, Viral; D002999:Clone Cells; D004247:DNA / Q000032:analysis; D004279:DNA, Viral / Q000032:analysis; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D008168:Lung; D013539:Simian virus 40 / Q000235:genetics*
D004279:DNA, Viral; D004247:DNA