Sequences sufficient for correct regulation of Sgs-3 lie close to or within the gene.
The Drosophila melanogaster 68C chromosomal locus is the site of a prominent polytene chromosome puff that harbors the genes Sgs-3, Sgs-7 and Sgs-8. These genes code for proteins that are part of the salivary glue that Drosophila larvae secrete as a means of fixing themselves to an external substrate for the duration of the pre-pupal and pupal period. The 68C glue genes are regulated by the steroid hormone ecdysterone, with the hormone required for both initiation and cessation of gene expression during the third larval instar. Previous work has defined sequences sufficient for expression of abundant levels of Sgs-3 mRNA at the correct time and in the correct tissue. We show here that sequences sufficient for normal tissue- and stage-specific accumulation of Sgs-3 RNA, but adequate only for low levels of expression, lie within 130 bp of the 5' end of the gene, or within the gene.
D000818:Animals; D001483:Base Sequence; D004262:DNA Restriction Enzymes; D004331:Drosophila melanogaster / Q000235:genetics*; D005796:Genes; D005809:Genes, Regulator; D005967:Glue Proteins, Drosophila; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D012471:Salivary Proteins and Peptides / Q000235:genetics*; D014158:Transcription, Genetic
D005967:Glue Proteins, Drosophila; D012471:Salivary Proteins and Peptides; D004262:DNA Restriction Enzymes