title: Every ribosomal suppressor mutation in Aspergillus nidulans has a unique and highly pleiotropic phenotype.
abstract: 18 suppressors of alcR125 have been selected in Aspergillus nidulans. They have been located in genes as follows: 12 in suaA, 1 in suaB and 5 in suaC. Suppressors have been examined to see whether their phenotype is diagnostic for their genotype. Several new traits are described: conidial viability, cycloheximide resistance, fertility, suppression of niaD500, niaD501 and fwA1. These tests, added to those already in use, provide a battery of tests suitable for assigning suppressor mutations to physiological type (tRNA or ribosomal), and in one case to a specific gene since only suaA mutations suppressed fwA1. A very broad range of phenotypes was associated with suppressors such that every mutation had a unique phenotype. This indicates that the ribosomal suppressor mutations are in genes which code directly for ribosomal proteins, rather than genes which code for modifying enzymes.
mesh_terms: D001233:Aspergillus nidulans / Q000235:genetics* / Q000254:growth & development; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D005298:Fertility; D005656:Fungal Proteins / Q000235:genetics*; D005799:Genes, Dominant; D005800:Genes, Fungal; D008040:Genetic Linkage; D006238:Haploidy; D008826:Microbial Sensitivity Tests; D010641:Phenotype; D012343:RNA, Transfer / Q000235:genetics; D012269:Ribosomal Proteins / Q000235:genetics*; D013489:Suppression, Genetic; D013696:Temperature
chemical_list: D005656:Fungal Proteins; D012269:Ribosomal Proteins; D012343:RNA, Transfer