title: Replication of Aleutian disease virus in mink lymphocytes infected in vitro.
abstract: The kinetics of Aleutian disease virus (ADV) replication in mink lymphocytes was followed by the analysis of virus-specific antigens, infectious virus, and viral DNA. Stimulation with pokeweed mitogen (PWM; 20 micrograms/ml) increased the synthesis of ADV DNA in cultivated cells of the B-cell fraction. Maximum virus titers [10(6.5) fluorescence-forming units (FFU)/ml] were achieved after incubating infected cells for 60 h at 32 degrees. At this time, 10.4% of the cells in the B-cell-enriched fraction contained ADV-specific antigens and there was an average of 125 ADV genome equivalents per antigen-positive cell. ADV replication also was detected in T-cell-enriched fractions (with up to 10(5.3) FFU/ml, 5.3% of cells were antigen-positive, with 20 ADV genome equivalents per antigen-positive cell), but was 10 times lower compared with ADV replication in the B-cell fraction.
mesh_terms: D000454:Aleutian Mink Disease Virus / Q000302:isolation & purification / Q000502:physiology*; D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002478:Cells, Cultured; D004261:DNA Replication; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D007700:Kinetics; D008214:Lymphocytes / Q000382:microbiology; D008297:Male; D008907:Mink; D010321:Parvoviridae / Q000502:physiology*; D014776:Virus Cultivation; D014779:Virus Replication
chemical_list: D000956:Antigens, Viral; D004279:DNA, Viral