Ferredoxin-thioredoxin reductase, an iron-sulfur enzyme linking light to enzyme regulation in oxygenic photosynthesis: purification and properties of the enzyme from C3, C4, and cyanobacterial species.
Ferredoxin-thioredoxin reductase (FTR), an enzyme involved in the light regulation of chloroplast enzymes, was purified to homogeneity from leaves of spinach (a C3 plant) and corn (a C4 plant) and from cells of a cyanobacterium (Nostoc muscorum). The enzyme is a yellowish brown iron-sulfur protein, containing four nonheme iron and labile sulfide groups, that catalyzes the activation of NADP-malate dehydrogenase and fructose 1,6-bisphosphatase in the presence of ferredoxin and of thioredoxin m and f, respectively. FTR is synonymous with the protein earlier called ferralterin. FTR showed an Mr of about 30,000 (determined by sedimentation equilibrium ultracentrifugation, amino acid composition, gel filtration, and gradient gel electrophoresis) and was composed of two dissimilar subunits (as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis). One of the FTR subunits from each source was similar both in Mr (about 13,000) and immunological properties, while the other subunit (of variable molecular weight) was characteristic of a particular organism. The similar subunit contained a disulfide group that was rapidly reduced by a dithiol (dithiothreitol) but not by monothiols (2-mercaptoethanol or reduced glutathione). Homogeneous FTR formed a tight noncovalent complex with ferredoxin on affinity columns. The basis for the structural variation in the different FTR enzymes remains to be determined.
D054479:Chloroplast Thioredoxins; D002736:Chloroplasts / Q000502:physiology*; D003300:Copper / Q000494:pharmacology; D003429:Cross Reactions; D000458:Cyanobacteria / Q000201:enzymology*; D003958:Diamide / Q000494:pharmacology; D005288:Ferredoxins / Q000378:metabolism; D006597:Fructose-Bisphosphatase / Q000378:metabolism; D007506:Iron-Sulfur Proteins / Q000502:physiology; D007526:Isoelectric Point; D008027:Light; D046911:Macromolecular Substances; D008291:Malate Dehydrogenase / Q000378:metabolism; D008970:Molecular Weight; D010088:Oxidoreductases / Q000302:isolation & purification* / Q000378:metabolism; D010788:Photosynthesis; D010940:Plant Proteins / Q000502:physiology; D010944:Plants; D013045:Species Specificity; D013057:Spectrum Analysis; D013879:Thioredoxins / Q000378:metabolism
D054479:Chloroplast Thioredoxins; D005288:Ferredoxins; D007506:Iron-Sulfur Proteins; D046911:Macromolecular Substances; D010940:Plant Proteins; D003958:Diamide; D013879:Thioredoxins; D003300:Copper; D010088:Oxidoreductases; D008291:Malate Dehydrogenase; C021588:ferredoxin-thioredoxin reductase; D006597:Fructose-Bisphosphatase