Transforming potential of DNA of the human PLC/PRF/5 hepatoma cell line.
DNA of the human PLC/PRF/5 hepatoma cell line can induce the appearance of colonies on soft agar after transfection of BK-BK cells (BALB/c mouse kidney cells partially transformed by BK virus). Blot hybridization analyses indicate that the transformants contained the hepatitis B virus DNA sequence. This sequence disappeared during serial passage of transformants. The amplification and rearrangement of the integrated BK virus genome appears to be specifically associated with transformation.
D000818:Animals; D001739:BK Virus / Q000235:genetics*; D006528:Carcinoma, Hepatocellular / Q000235:genetics*; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D002472:Cell Transformation, Viral; D004273:DNA, Neoplasm / Q000235:genetics*; D004279:DNA, Viral / Q000378:metabolism; D006515:Hepatitis B virus / Q000235:genetics; D006801:Humans; D008113:Liver Neoplasms; D051379:Mice; D009857:Oncogenes; D011120:Polyomavirus / Q000235:genetics*; D018139:Simplexvirus / Q000235:genetics
D004273:DNA, Neoplasm; D004279:DNA, Viral