Cloning and sequence analysis of the human brain beta-adrenergic receptor. Evolutionary relationship to rodent and avian beta-receptors and porcine muscarinic receptors.
Two cDNA clones, lambda-CLFV-108 and lambda-CLFV-119, encoding for the beta-adrenergic receptor, have been isolated from a human brain stem cDNA library. One human genomic clone, LCV-517 (20 kb), was characterized by restriction mapping and partial sequencing. The human brain beta-receptor consists of 413 amino acids with a calculated Mr of 46480. The gene contains three potential glucocorticoid receptor-binding sites. The beta-receptor expressed in human brain was homology with rodent (88%) and avian (52%) beta-receptors and with porcine muscarinic cholinergic receptors (31%), supporting our proposal [(1984) Proc. Natl. Acad. Sci. USA 81, 272 276] that adrenergic and muscarinic cholinergic receptors are structurally related. This represents the first cloning of a neurotransmitter receptor gene from human brain.
D000595:Amino Acid Sequence; D000818:Animals; D005075:Biological Evolution; D001717:Birds / Q000378:metabolism; D001921:Brain / Q000378:metabolism*; D003001:Cloning, Molecular; D006801:Humans; D007231:Infant, Newborn; D008961:Models, Structural; D011943:Receptors, Adrenergic, beta / Q000235:genetics*; D011976:Receptors, Muscarinic / Q000235:genetics; D012377:Rodentia / Q000378:metabolism; D013552:Swine / Q000378:metabolism
D011943:Receptors, Adrenergic, beta; D011976:Receptors, Muscarinic