Effect of DNA sequence changes on UV mutability of a purine anticodon triplet of glyU cloned on M13 phage.
Mutant forms of the glyU (glycyl tRNA) gene cloned in M13mp8 were subjected to uninduced targeted UV mutagenesis; i.e. phage particles were irradiated and used to infect unirradiated umuC+ or irradiated umuC mutant cells. The irradiated phage carried GAG at the anticodon triplet and transitions to GAA were scored. The uninduced targeted mutation rate was reduced by altering the sequence of the gene in the vicinity of the target purine (Pu) residue. In particular a triplet of pyrimidines (PyPyPy) 5' to the target G was changed to PyPuPy in order to prevent formation of cyclcobutane and 6-4 pyrimidine dimers close to the target. On this basis we suggest a mechanism for one type of uninduced regionally targeted UV mutagenesis.
D000926:Anticodon; D001483:Base Sequence; D003001:Cloning, Molecular; D003090:Coliphages / Q000235:genetics*; D004307:Dose-Response Relationship, Radiation; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial / Q000528:radiation effects*; D008969:Molecular Sequence Data; D009154:Mutation; D012343:RNA, Transfer; D012345:RNA, Transfer, Amino Acid-Specific / Q000235:genetics*; D012353:RNA, Transfer, Gly / Q000235:genetics*; D014466:Ultraviolet Rays
D000926:Anticodon; D012345:RNA, Transfer, Amino Acid-Specific; D012353:RNA, Transfer, Gly; D012343:RNA, Transfer