title: Nucleotide sequence of a cDNA clone of RNA segment 10 of bluetongue virus (serotype 10).
abstract: The complete sequence of the double-stranded RNA segment that codes for a non-structural protein (P8) of bluetongue virus serotype 10 has been determined from a cDNA clone inserted into the plasmid pBR322. The segment 10 RNA of the virus (S10 RNA) is deduced to be 822 base pairs long (0.5 X 10(6) daltons) and has an open reading frame in one strand capable of coding for a protein with a calculated size of 25,572 daltons (229 amino acids) and a net charge of +5.5 at neutral pH.
mesh_terms: D000596:Amino Acids / Q000032:analysis; D001483:Base Sequence; D001820:Bluetongue virus / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA; D005814:Genes, Viral; D008970:Molecular Weight; D012367:RNA, Viral / Q000235:genetics*; D012087:Reoviridae / Q000235:genetics*; D014764:Viral Proteins / Q000032:analysis / Q000235:genetics
chemical_list: D000596:Amino Acids; D012367:RNA, Viral; D014764:Viral Proteins; D004247:DNA