[Heterogeneity of nuclear proteins from HeLa cells specifically binding to the Alu sequence].
Nuclear proteins from HeLa cells specifically binding to the Alu-repeat cloned in the plasmid Blur8 have been studied. 0.35 M nuclear extract proteins have been separated on DEAE-cellulose. The presence of DNA-binding proteins has been found in all fractions by the technique of DNA-binding on nitrocellulose filters. The labelled restricted DNA of the plasmid Blur8 was incubated with the proteins of different fractions with the subsequent identification of specific Alu-protein complexes in polyacrylamide gel at low ionic strength. At least two proteins have been found to have the different affinity to Alu-repeat. Various functions of Alu-repeats and the possibility of their participation in the initiation of DNA replication are discussed.
D004247:DNA / Q000235:genetics*; D004261:DNA Replication; D004262:DNA Restriction Enzymes; D004268:DNA-Binding Proteins / Q000235:genetics*; D015252:Deoxyribonucleases, Type II Site-Specific; D006367:HeLa Cells; D006801:Humans; D009698:Nucleoproteins / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid
D004268:DNA-Binding Proteins; D009698:Nucleoproteins; D004247:DNA; D004262:DNA Restriction Enzymes; C024669:endodeoxyribonuclease AluI; D015252:Deoxyribonucleases, Type II Site-Specific
Geterogennost' iadernykh belkov kletok HeLa, spetsificheski sviazyvaiushchikh Alu-posledovatel'nost'.