[Immunohistochemical study of carcinoembryonic antigen (CEA) in normal and embryonic human tissues using a CEA-specific oncoprecipitin, krustacin].
Using immunoperoxidase technique, antibodies to CEA (Ab) were compared to a glycoprotein krustacin (Kr) extracted from Pagurus prideauxii, which has an ability to precipitate specifically CEA. It was found that Kr and Ab reacted in a similar manner with embryonic and normal gastrointestinal tissues, revealing practically identical localization of the antigen in the tissues and cells. It was possible, however, to note some quantitative and qualitative differences in the distribution of antigen, which showed that Kr and Ab reacted with different determinants of the CEA molecule.
D000328:Adult; D000818:Animals; D000906:Antibodies / Q000032:analysis; D003386:Brachyura; D002272:Carcinoembryonic Antigen / Q000032:analysis* / Q000276:immunology; D004622:Embryo, Mammalian / Q000032:analysis; D004625:Embryo, Nonmammalian; D005333:Fetus / Q000032:analysis; D006023:Glycoproteins; D006801:Humans; D007124:Immunoenzyme Techniques; D066298:In Vitro Techniques; D007447:Invertebrate Hormones / Q000032:analysis* / Q000276:immunology; D011234:Precipitins
D000906:Antibodies; D002272:Carcinoembryonic Antigen; D006023:Glycoproteins; D007447:Invertebrate Hormones; D011234:Precipitins
Immunogistokhimicheskoe izuchenie rakovo-émbrional'nogo antigena (REA) v normal'nykh i émbrional'nykh tkaniakh cheloveka s pomozhch'iu REA-spetsificheskogo onkopretsipitina--krustatsina.