Specific purification of monoclonal anti-DNA antibodies from culture medium using a DNA-coupled Sepharose 4B affinity column.
Human monoclonal antibodies against DNA were specifically purified from the culture medium of an EBV transformant of SLE patients' lymphocytes using a DNA-coupled Sepharose 4B affinity column. The monoclonal antibodies were eluted from the column with 5% dimethylsulfoxide (pH 10.7) containing 0.5 M NaCl without loss of immunological activity and without contamination by other proteins.
D000974:Antibodies, Antinuclear / Q000302:isolation & purification*; D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D002846:Chromatography, Affinity / Q000379:methods*; D003470:Culture Media / Q000032:analysis; D004247:DNA / Q000276:immunology; D004121:Dimethyl Sulfoxide; D004854:Herpesvirus 4, Human; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology; D012685:Sepharose
D000974:Antibodies, Antinuclear; D000911:Antibodies, Monoclonal; D003470:Culture Media; D004247:DNA; D012685:Sepharose; D004121:Dimethyl Sulfoxide