title: Stimulation of collagenase production in human synovial fibroblast cultures by poly (I). poly (C).
abstract: Poly (I). poly (C) was found to induce collagenase secretion in both "normal" and "rheumatoid" synovial fibroblast cultures. Induction was time dependent, was maximal at 20-50 micrograms/ml poly (I). poly (C) and was dependent on de novo synthesis. Induction was prevented by hydrocortisone (0.1 microgram/ml) but inhibition of prostaglandin E production by diclofenac (0.2 microgram/ml) or ibuprofen (0.1 microgram/ml) did not affect collagenase production. Collagenase induction was accompanied by stimulation of hyaluronic acid and prostaglandin E production. This in vitro system may represent a model for the study of pathological secretory activities in the inflamed joint.
mesh_terms: D002460:Cell Line; D002478:Cells, Cultured; D004790:Enzyme Induction; D005347:Fibroblasts / Q000201:enzymology; D006801:Humans; D006820:Hyaluronic Acid / Q000096:biosynthesis; D003012:Microbial Collagenase / Q000096:biosynthesis*; D011070:Poly I-C / Q000494:pharmacology*; D011458:Prostaglandins E / Q000096:biosynthesis; D013583:Synovial Membrane / Q000201:enzymology*
chemical_list: D011458:Prostaglandins E; D006820:Hyaluronic Acid; D003012:Microbial Collagenase; D011070:Poly I-C