Use of double spin-labeled histones to monitor histone-chromatin integration.
Tyrosine-specific nitroxide spin labels have been synthesized that utilize either deuterium or deuterium and [15N] isotopic substitution within the nitroxide ring. These probes have been used to differentially spin label and simultaneously monitor both histones H1 and H5, during the displacement of endogenous spin-labeled H1 from reconstituted chromatin by exogenously added spin-labeled H5.
D000818:Animals; D002645:Chickens; D002843:Chromatin / Q000378:metabolism*; D003497:Cyclic N-Oxides; D004578:Electron Spin Resonance Spectroscopy; D006657:Histones / Q000378:metabolism*; D007093:Imidazoles; D066298:In Vitro Techniques; D009707:Nucleosomes / Q000648:ultrastructure*; D013113:Spin Labels; D014443:Tyrosine
D002843:Chromatin; D003497:Cyclic N-Oxides; D006657:Histones; D007093:Imidazoles; D009707:Nucleosomes; D013113:Spin Labels; D014443:Tyrosine; C018676:N-(2,2',5,5'-tetramethyl-3-carboxypyrroline-1-oxyl)imidazole