[Genetic transformation of somatic cells. XIII. Limited replication of DNA containing a fragment of human satellite DNA III in mouse cells].
The plasmids containing the variant sequence of human satellite III "rescued" after replication in Chinese hamster cells were transfected into Chinese hamster, mouse and human cells by DEAE-dextran method. Several days after transfection extrachromosomal fractions were isolated, treated with DpnI, transformed into E. coli. In mouse cells, transformed with oncogene v-myc, after transient transfection of HS3-containing plasmids the appearance of rearranged and non-rearranged DpnI-resistant plasmids has been found. At the same time MboI-sensitive plasmids were not found in this material. The data suggest a limited replication (1 round) of HS3-containing plasmids in mouse cells transformed with oncogene v-myc.
D000818:Animals; D002478:Cells, Cultured; D006224:Cricetinae; D004261:DNA Replication; D004251:DNA Transposable Elements; D004276:DNA, Satellite / Q000235:genetics*; D005111:Extrachromosomal Inheritance; D006801:Humans; D051379:Mice; D009857:Oncogenes; D010957:Plasmids; D013997:Time Factors; D014162:Transfection; D014170:Transformation, Genetic
D004251:DNA Transposable Elements; D004276:DNA, Satellite
Geneticheskaia transformatsiia somaticheskikh kletok. XIII. Ogranichennaia replikatsiia DNK, soderzhashcheĭ fragment satellitnoĭ DNK III cheloveka, v kletkakh mysheĭ.