Yeast pyruvate carboxylase: gene isolation.
To improve our understanding of pyruvate carboxylase (PC)(EC 6.4.1.1) structure and the evolution of the biotin-dependent carboxylases we have isolated and sequenced a yeast (Saccharomyces cerevisiae) genomic DNA fragment encoding PC. The identity of the cloned gene was confirmed by comparing the encoded protein with the sequence of a 26 amino acid biotin-containing peptide isolated from yeast PC. The yeast PC sequence is homologous (43% amino acid homology) to the rat PC sequence, although the carboxyl-terminus was found to be 44 residues from the biotinyl-lysine whereas in all biotin carboxylases sequenced to date the biotin is 35 residues from the carboxyl-terminus.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004262:DNA Restriction Enzymes; D004271:DNA, Fungal / Q000302:isolation & purification; D005796:Genes; D005800:Genes, Fungal; D011766:Pyruvate Carboxylase / Q000235:genetics*; D051381:Rats; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
D004271:DNA, Fungal; D004262:DNA Restriction Enzymes; D011766:Pyruvate Carboxylase