title: Recombinant plasmid DNA variation of Clostridium oncolyticum--model experiments of cancerostatic gene transfer.
abstract: The specific germinating capacity of spores of Clostridium oncolyticum in tumours in vivo, and various reports on concomitant partial oncolysis (tumour lysis) have prompted us to propose a concept of equipping C. oncolyticum with genes of other organisms producing cancerostatics. As an example we used Colicin E3, whose structural genes lies in the E. coli plasmid pCo1E3-CA38. After in vitro recombination of restrictase EcoRI-fragmented pCo1E3-CA38 DNA with an uncharacterized plasmid fraction from C. concolyticum a plasmid-free strain of C. oncolyticum was infected with recombinant DNA. A special microbiological selection system allows the identification of C. oncolyticum clones with Colicin E3-similar formation of cleared haloes.
mesh_terms: D003001:Cloning, Molecular; D003013:Clostridium / Q000235:genetics* / Q000502:physiology; D003087:Colicins / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics / Q000648:ultrastructure; D004274:DNA, Recombinant; D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli / Q000235:genetics; D014644:Genetic Variation; D005822:Genetic Vectors; D008854:Microscopy, Electron; D010957:Plasmids; D013171:Spores, Bacterial; D014162:Transfection
chemical_list: D003087:Colicins; D004269:DNA, Bacterial; D004274:DNA, Recombinant