Reassortment of human rotavirus possessing genome rearrangements with bovine rotavirus: evidence for host cell selection.
Mixed infections of secondary rhesus monkey kidney cells with human rotaviruses carrying rearranged genomes and with bovine rotavirus yielded a high percentage of reassortants. The genotypes of 511 plaque-purified clones raised in either MA104 or BSC-1 cells have been determined and the frequencies of different genotypes have been calculated. It was found that reassortants did not emerge at random; there was non-random association of certain genes; the cell line used to isolate reassortants influenced the result, i.e. host cell factors had a selective effect on a recombinational mixture.
D000818:Animals; D002417:Cattle; D002460:Cell Line; D002522:Chlorocebus aethiops; D004247:DNA / Q000032:analysis; D005787:Gene Frequency; D005814:Genes, Viral; D006801:Humans; D007668:Kidney; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000302:isolation & purification; D012401:Rotavirus / Q000235:genetics*; D013045:Species Specificity
D012367:RNA, Viral; D004247:DNA