title: The effect of parathyroid hormone (1-34) on cyclic AMP level, ornithine decarboxylase activity, and glycosaminoglycan synthesis of chondrocytes from mandibular condylar cartilage, nasal septal cartilage, and spheno-occipital synchondrosis in culture.
abstract: Previously, we reported methods for isolating chondrocytes from the craniofacial complex and their culture in vitro. The response of these chondrocyte cultures to bovine parathyroid hormone (1-34) (PTH) has now been investigated. PTH stimulated glycosaminoglycan (GAG) synthesis, a characteristic of the cartilage phenotype in cultured chondrocytes isolated from mandibular condylar cartilage (MCC), nasal septal cartilage (NSC), and spheno-occipital synchondrosis (SOS). These stimulations of GAG synthesis by PTH were dose-dependent. PTH also increased accumulation of cyclic AMP (cAMP) and the activity of ornithine decarboxylase (ODC), a rate-limiting enzyme in polyamine biosynthesis. However, PTH did not stimulate DNA synthesis. The increases in the cAMP level, ODC activity, and GAG synthesis after addition of PTH (10(-7) mol/L) were greatest in MCC-chondrocytes and least in NSC-chondrocytes. The difference in the responses to PTH of these three types of chondrocytes may reflect differences of the characteristics of these cells in vivo.
mesh_terms: D000818:Animals; D002356:Cartilage / Q000166:cytology* / Q000201:enzymology / Q000378:metabolism; D002478:Cells, Cultured; D003393:Cranial Sutures / Q000166:cytology; D000242:Cyclic AMP / Q000378:metabolism*; D004247:DNA / Q000096:biosynthesis; D006025:Glycosaminoglycans / Q000096:biosynthesis*; D008335:Mandibular Condyle / Q000166:cytology; D009300:Nasal Septum / Q000166:cytology; D009777:Occipital Bone / Q000166:cytology; D009955:Ornithine Decarboxylase / Q000378:metabolism*; D010281:Parathyroid Hormone / Q000494:pharmacology*; D011817:Rabbits; D013100:Sphenoid Bone / Q000166:cytology
chemical_list: D006025:Glycosaminoglycans; D010281:Parathyroid Hormone; D004247:DNA; D000242:Cyclic AMP; D009955:Ornithine Decarboxylase