Receptor for 1,25-dihydroxyvitamin D in a vascular smooth muscle cell line derived from rat aorta.
The presence of a specific receptor for 1,25-dihydroxyvitamin D (1,25(OH)2D) was investigated in a cell line A7r5 derived from fetal aorta. 1,25(OH)2[3H]D3 binding to cytosol was saturated at 0.6-1 nM, and Scatchard analysis yielded dissociation constant and binding sites, (3.02 +/- 0.4) X 10(-11) M and 33.9 +/- 5.8 fmol/mg protein, respectively. Sucrose density gradient analysis revealed the sedimentation constant 3.2 S. Furthermore, the receptor protein had affinity for DNA-cellulose column and eluted with 0.2 M KCl. These data suggest that vascular smooth muscle cell may be a target tissue of vitamin D.
D000818:Animals; D001011:Aorta / Q000032:analysis; D002117:Calcitriol / Q000378:metabolism; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D007700:Kinetics; D009131:Muscle, Smooth, Vascular / Q000032:analysis*; D051381:Rats; D018167:Receptors, Calcitriol; D011987:Receptors, Steroid / Q000032:analysis*
D018167:Receptors, Calcitriol; D011987:Receptors, Steroid; D000252:Calcium-Transporting ATPases; D002117:Calcitriol