title: Acetone/periodate-lysine-paraformaldehyde (PLP) fixation and improved morphology of cryostat sections for immunohistochemistry.
abstract: Cryostat sections are extensively used in the study of lymphoid tissues because the majority of antigens do not survive routine fixation and processing. A major disadvantage of cryostat section immunohistochemistry is their poor morphology. The use of periodate-lysine-paraformaldehyde (PLP) fixation regimes for cryostat sections was evaluated and compared with conventional acetone immersion. This fixation gives excellent morphology but poor immunostaining. The quality of immunostaining with a panel of 31 antibodies was good with brief acetone fixation followed by PLP fixation. Morphological preservation was very good. We suggest that acetone-PLP fixation is an improvement over acetone alone for cryostat section immunohistochemistry.
mesh_terms: D000096:Acetone; D000954:Antigens, Surface / Q000032:analysis; D005404:Fixatives; D005557:Formaldehyde; D005629:Frozen Sections; D006652:Histological Techniques; D006801:Humans; D007124:Immunoenzyme Techniques; D008239:Lysine; D008867:Microtomy; D014066:Palatine Tonsil / Q000166:cytology / Q000276:immunology; D010504:Periodic Acid
chemical_list: D000954:Antigens, Surface; D005404:Fixatives; C046311:periodate-lysine-paraformaldehyde; D010504:Periodic Acid; D000096:Acetone; D005557:Formaldehyde; D008239:Lysine