Tissue factor-dependent activation of platelets by cells and microvesicles of SK-OS-10 human osteogenic sarcoma cell line.
Cultured SK-OS-10 cells (human osteogenic sarcoma metastatic to lung) shed microvesicles (dia. 300-1000 nm) that contained procoagulant and proaggregatory activities inhibitable by hirudin, by anti-tissue factor antibody and by phospholipase A2. These results show that SK-OS-10 cells belong to a group including U87MG human glioblastoma and HL-60 promyelocytic leukemia in which these activities are due to a thrombin-dependent mechanism arising from the presence of tissue factor on the surface of the tumor cells and their shed microvesicles.
D000906:Antibodies; D001792:Blood Platelets / Q000502:physiology*; D002460:Cell Line; D003402:Creatine Kinase / Q000494:pharmacology; D006629:Hirudins / Q000494:pharmacology; D006801:Humans; D002479:Inclusion Bodies / Q000378:metabolism; D008175:Lung Neoplasms / Q000556:secondary*; D012516:Osteosarcoma / Q000378:metabolism* / Q000473:pathology; D010725:Phosphocreatine / Q000494:pharmacology; D010740:Phospholipases / Q000494:pharmacology; D010972:Platelet Activating Factor; D010974:Platelet Aggregation / Q000187:drug effects; D013925:Thromboplastin / Q000276:immunology / Q000494:pharmacology*
D000906:Antibodies; D006629:Hirudins; D010972:Platelet Activating Factor; D010725:Phosphocreatine; D013925:Thromboplastin; D003402:Creatine Kinase; D010740:Phospholipases