Possible involvement of a Na+/H+ antiporter in the stimulation of hexose transport in Swiss 3T3 cells by a phorbol ester and growth factors.
Phorbol-12,13-dibutyrate, epidermal growth factor, and insulin raised the intracellular pH ([pH]i), presumably through the activation of a Na+/H+ antiporter. Addition of amiloride or replacement of extra-cellular Na+ by choline which abolishes the cytoplasmic alkalinization prevented the stimulation of hexose transport by these agents. Furthermore, monensin, a Na+/H+ ionophore which increases the [pH]i, stimulated hexose transport. This stimulation was also prevented by the replacement of extra-cellular Na+ by choline. These observations suggest that stimulation of the Na+/H+ antiporter may have stimulated the increase in hexose transport.
D000584:Amiloride / Q000494:pharmacology; D000818:Animals; D002352:Carrier Proteins / Q000502:physiology*; D002460:Cell Line; D004815:Epidermal Growth Factor / Q000494:pharmacology; D006133:Growth Substances / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D007328:Insulin / Q000494:pharmacology; D051379:Mice; D008985:Monensin / Q000494:pharmacology; D009004:Monosaccharide Transport Proteins / Q000502:physiology*; D015240:Phorbol 12,13-Dibutyrate; D010703:Phorbol Esters / Q000494:pharmacology*; D017923:Sodium-Hydrogen Exchangers
D002352:Carrier Proteins; D006133:Growth Substances; D007328:Insulin; D009004:Monosaccharide Transport Proteins; D010703:Phorbol Esters; D017923:Sodium-Hydrogen Exchangers; D015240:Phorbol 12,13-Dibutyrate; D004815:Epidermal Growth Factor; D000584:Amiloride; D008985:Monensin