Structure of the O-chain of the lipopolysaccharide of Haemophilus pleuropneumoniae serotype 1.
By phenol-water extraction an aqueous-phase soluble cellular lipopolysaccharide was isolated from Haemophilus pleuro-pneumoniae serotype 1. It was shown by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, hydrolysis, methylation, and both one- and two-dimensional 1H and 13C nuclear magnetic resonance studies to be an S-type lipopolysaccharide, which could be cleaved to yield a lipid A and an O-chain polysaccharide identified as a high molecular weight branched polymer of a tetrasaccharide repeating unit having the structure: (Formula: see text).
D000107:Acetylation; D002240:Carbohydrate Sequence; D003641:Deamination; D006190:Haemophilus / Q000032:analysis*; D008050:Lipid A; D008070:Lipopolysaccharides; D009682:Magnetic Resonance Spectroscopy; D010084:Oxidation-Reduction; D010504:Periodic Acid; D011135:Polysaccharides, Bacterial
D008050:Lipid A; D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial; D010504:Periodic Acid