[Autonomously replicating sequence activity of kinetoplast DNA in yeast cells].
Different fragments of the maxicircle of the kinetoplast DNA (kpDNA) from Crithidia oncopelti were cloned (Fig. 1, 2) and tested for ARS-activity (ARS, autonomously replicating sequences). ARS-activity was expressed as number of transformed yeast cells per microgram plasmid DNA, as number of transformed cells per number of plasmid molecules (transformation efficiency, TE) and as number of transformed cells per kilobase pair of cloned kpDNA (specific transformation efficiency, TES) (Fig. 3, Table 1). All DNA fragments studied showed ARS-activity. Large fragments exhibited higher ARS-activities than their smaller subfragments. The number of fragments showing ARS activity and their distribution within the maxicircles (Fig. 4) suggest that there was no strong correlation between sites with ARS-activity and the replication origin of kpDNA.
D000818:Animals; D001426:Bacterial Proteins; D001483:Base Sequence; D003001:Cloning, Molecular; D003421:Crithidia / Q000235:genetics*; D004261:DNA Replication; D004262:DNA Restriction Enzymes; D004270:DNA, Circular / Q000032:analysis / Q000096:biosynthesis*; D018105:DNA, Kinetoplast; D015247:Deoxyribonuclease HindIII; D015252:Deoxyribonucleases, Type II Site-Specific; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014170:Transformation, Genetic
D001426:Bacterial Proteins; D004270:DNA, Circular; D018105:DNA, Kinetoplast; D004262:DNA Restriction Enzymes; D015247:Deoxyribonuclease HindIII; C024404:BglII endonuclease; D015252:Deoxyribonucleases, Type II Site-Specific
ARS-Aktivität von Kinetoplasten-DNA in Hefezellen.