title: [Substrate specificity of restriction endonuclease VspI].
abstract: The recognition sequence and cleavage point of restriction endonuclease VspI have been determined as 5'-AT decreases TAAT. This enzyme is not isoschizomer of any known restriction endonucleases. DNA pBR322 contains a single VspI recognition sequence in position 3539. Therefore this enzyme may be used for cloning DNA in the VspI site in AmpR-gene of pBR322.
mesh_terms: D001483:Base Sequence; D004262:DNA Restriction Enzymes / Q000235:genetics* / Q000302:isolation & purification; D015252:Deoxyribonucleases, Type II Site-Specific; D013379:Substrate Specificity; D014733:Vibrio / Q000201:enzymology*
chemical_list: D004262:DNA Restriction Enzymes; C052712:endodeoxyribonuclease VspI; D015252:Deoxyribonucleases, Type II Site-Specific
vernacular_title: Opredelenie substratnoĭ spetsifichnosti restriktsionnoĭ éndonukleazy VspI.