Comparison of Isolab Quik-Sep and Quik-Sep System II for separation of immunoglobulins G and M.
Use of Quik-Sep System II (QSII) ion-exchange chromatography increased the detection of CMV-directed immunoglobulin M (IgM) compared to the prototype Quik-Sep System (QSI) (27 of 87 [31%] IgM-positive versus 20 of 87 [24%]). However, an increase in the number of false-positive rheumatoid factor-containing sera was found (15/52 [29%] versus 8/52 [15%]). Total immunoglobulin G (IgG) and IgM concentrations were higher in the QSII eluates compared to those from QSI, although total IgM recovery did not exceed 36% in either system.
D002852:Chromatography, Ion Exchange; D003587:Cytomegalovirus / Q000276:immunology*; D003586:Cytomegalovirus Infections / Q000175:diagnosis; D004797:Enzyme-Linked Immunosorbent Assay; D005189:False Positive Reactions; D005455:Fluorescent Antibody Technique; D006801:Humans; D005779:Immunodiffusion; D007074:Immunoglobulin G / Q000032:analysis*; D007075:Immunoglobulin M / Q000032:analysis*; D011237:Predictive Value of Tests; D012217:Rheumatoid Factor / Q000032:analysis
D007074:Immunoglobulin G; D007075:Immunoglobulin M; D012217:Rheumatoid Factor