title: The influence of microtiter plates from different suppliers on lymphocyte proliferation.
abstract: Microtiter plates have been popularly used for lymphocyte culture, but the influence of culture plates from different sources has not been investigated. In this study, the degree of mitogen-induced cell proliferation was investigated using six different brands of flat-bottomed plates. Lymphocytes from twelve normal donors were cultured for 96 hours with several mitogens including PHA, Con A and PWM. Spontaneous cell proliferation was slow and it did not differ significantly among the different plates. However, mitogen-induced cell proliferation showed a wide variation among the six types of plates used. The importance of selecting certain kinds of plates for specific purposes is emphasized.
mesh_terms: D000328:Adult; D003208:Concanavalin A / Q000494:pharmacology; D005260:Female; D006801:Humans; D007158:Immunologic Techniques / Q000295:instrumentation*; D066298:In Vitro Techniques; D008213:Lymphocyte Activation; D008297:Male; D010835:Phytohemagglutinins / Q000494:pharmacology; D011043:Pokeweed Mitogens / Q000494:pharmacology
chemical_list: D010835:Phytohemagglutinins; D011043:Pokeweed Mitogens; D003208:Concanavalin A