Mechanism of NaCl transport-stimulated prostaglandin formation in MDCK cells.
Recently we have found that stimulation of NaCl transport in high-resistance MDCK cells enhances their prostaglandin formation. In the present study, we investigated the mechanisms by which prostaglandin formation could be linked to the ion transport in these cells. We found that stimulation of transport caused a transient stimulation of prostaglandin formation lasting 5-10 min. The rise in prostaglandin formation was paralleled by a rise of free intracellular arachidonic acid. Analysis of membrane lipids revealed that the rise of free arachidonic acid was paralleled by a loss of arachidonic acid from polyphosphoinositides. We failed to obtain indications for the stimulation of calcium-dependent phospholipase A2. However, we did obtain evidence that the incorporation of arachidonic acid into phospholipids was diminished during stimulation of ion transport, indicating a decreased rate of reesterification. Despite the fact that there was no significant fall in total cellular ATP on stimulation of ion transport, we found a high and transient rise of lactate production of the cells on stimulation of the ion transport indicating an alteration of the ADP/ATP ratio. Moreover, prostaglandin formation and lactate formation were linearly correlated in this situation. When glucose utilization was inhibited by mannoheptulose, the rise in lactate formation was abolished, whereas that of PG formation was unaltered, indicating that lactate formation and prostaglandin formation were not causally linked on stimulation of ion transport. Our results suggest that an increase in the rate of sodium chloride transport by MDCK cells stimulates formation by an inhibition of reesterification of free arachidonic acid.(ABSTRACT TRUNCATED AT 250 WORDS)
D000255:Adenosine Triphosphate / Q000378:metabolism; D000654:Amobarbital / Q000494:pharmacology; D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism; D002460:Cell Line; D005576:Colforsin / Q000494:pharmacology; D004075:Diglycerides / Q000378:metabolism; D004285:Dogs; D005665:Furosemide / Q000494:pharmacology; D007668:Kidney / Q000378:metabolism*; D007773:Lactates / Q000378:metabolism; D008563:Membrane Lipids / Q000378:metabolism; D010042:Ouabain / Q000494:pharmacology; D010716:Phosphatidylinositols / Q000378:metabolism; D011453:Prostaglandins / Q000378:metabolism*; D012402:Rotenone / Q000494:pharmacology; D012965:Sodium Chloride / Q000378:metabolism
D001095:Arachidonic Acids; D004075:Diglycerides; D007773:Lactates; D008563:Membrane Lipids; D010716:Phosphatidylinositols; D011453:Prostaglandins; D012402:Rotenone; D005576:Colforsin; D016718:Arachidonic Acid; D012965:Sodium Chloride; D010042:Ouabain; D005665:Furosemide; D000255:Adenosine Triphosphate; D000654:Amobarbital