A simplified method for monitoring the cytochrome aa3 redox state in bilateral cortical areas of unanesthetized cats.
We describe a versatile optical system that enables the simultaneous monitoring of the redox state of cytochrome c oxidase (cytochrome aa3) in two homologous cortical areas under chronically implanted windows in cats. A single light source, broad bandpass primary filters, light-conducting rods, and narrow-bandpass interference detecting filters are employed. We observed reproducible responses of the cytochrome redox state and blood volume to carotid occlusion and terminal anoxia during anesthesia, and to graded doses of pentobarbital in awake animals.
D000818:Animals; D002339:Carotid Arteries / Q000601:surgery; D002415:Cats; D002540:Cerebral Cortex / Q000098:blood supply / Q000201:enzymology* / Q000601:surgery; D003250:Constriction; D004569:Electroencephalography; D003576:Electron Transport Complex IV / Q000378:metabolism*; D010084:Oxidation-Reduction; D013053:Spectrophotometry / Q000295:instrumentation* / Q000379:methods
D003576:Electron Transport Complex IV