The use of 12-hydroxyheptadecatrienoic acid (HHT) as an HPLC/spectrophotometric marker for cyclooxygenase pathway activity in resident rat peritoneal cells.
12-Hydroxyheptadecatrienoic acid (HHT), a UV chromophore, has been used to assess cyclooxygenase (CO) pathway metabolism of arachidonic acid (AA) by rat peritoneal cells. Simultaneous monitoring at 235 and 280 nm after HPLC permits the measurement of both CO and 5-lipoxygenase (5-LO) pathway fluxes in a single system.
D000818:Animals; D001094:Arachidonate 5-Lipoxygenase / Q000032:analysis; D001095:Arachidonic Acids / Q000378:metabolism; D001202:Ascitic Fluid / Q000166:cytology; D000001:Calcimycin / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D016861:Cyclooxygenase Inhibitors; D004804:Eosinophils / Q000187:drug effects / Q000201:enzymology; D005231:Fatty Acids, Unsaturated; D006893:Hydroxyeicosatetraenoic Acids / Q000032:analysis / Q000096:biosynthesis; D007975:Leukotriene B4 / Q000032:analysis / Q000096:biosynthesis; D016859:Lipoxygenase Inhibitors; D008264:Macrophages / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D008407:Mast Cells / Q000187:drug effects / Q000201:enzymology; D011448:Prostaglandin Antagonists / Q000494:pharmacology; D011451:Prostaglandin-Endoperoxide Synthases / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D012015:Reference Standards; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
D001095:Arachidonic Acids; D016861:Cyclooxygenase Inhibitors; D005231:Fatty Acids, Unsaturated; D006893:Hydroxyeicosatetraenoic Acids; D016859:Lipoxygenase Inhibitors; D011448:Prostaglandin Antagonists; D007975:Leukotriene B4; D000001:Calcimycin; C022022:5-hydroxy-6,8,11,14-eicosatetraenoic acid; C008033:12-hydroxy-5,8,10-heptadecatrienoic acid; D001094:Arachidonate 5-Lipoxygenase; D011451:Prostaglandin-Endoperoxide Synthases