Tumor necrosis factor stimulates gelatinase and collagenase production by granulation tissue in culture.
Tumor necrosis factor (TNF, 2.6 X 10(-11)-10(-9) M) caused an increase in the production of active gelatinase and latent collagenase by granulation tissue in culture. As determined by SDS-substrate polyacrylamide gel electrophoresis, granulation tissue produced mainly two species of gelatinase with molecular weights of 64 kDa and 57 kDa, and an additional 80-kDa gelatinase was produced by TNF treatment. The results suggest that TNF may play a role in a rapid collagen turnover in the carrageenin-induced granulation tissue in rats.
D000818:Animals; D003094:Collagen / Q000378:metabolism; D046508:Culture Techniques; D004591:Electrophoresis, Polyacrylamide Gel; D004790:Enzyme Induction / Q000187:drug effects; D005780:Gelatin / Q000378:metabolism; D018093:Gelatinases; D006023:Glycoproteins / Q000494:pharmacology*; D006097:Granulation Tissue / Q000201:enzymology*; D008297:Male; D003012:Microbial Collagenase / Q000096:biosynthesis*; D008970:Molecular Weight; D010434:Pepsin A / Q000096:biosynthesis*; D051381:Rats; D011919:Rats, Inbred Strains; D014409:Tumor Necrosis Factor-alpha
D006023:Glycoproteins; D014409:Tumor Necrosis Factor-alpha; D005780:Gelatin; D003094:Collagen; D010434:Pepsin A; D018093:Gelatinases; D003012:Microbial Collagenase