Reactivity of monoclonal antibodies to proteins of a neurotropic bovine herpesvirus 1 (BHV-1) strain and to proteins of representative BHV-1 strains.
15 monoclonal antibodies (McAbs) were induced with the neuropathogenic strain N 569 of bovine herpesvirus 1 type 3 (BHV-1.3). Nine of them could be shown by radioimmunoprecipitation assay to react with viral glycoproteins and two of these McAbs were able to neutralize strain N 569. The reactivity of these 15 monoclonals was compared with 11 monoclonal antibodies induced with a BHV-1.1 strain. The available monoclonal antibodies made it possible to characterize BHV-1.3 and to classify BHV-1 into three types, namely BHV-1.1, BHV-1.2 and BHV-1.3. This confirmed the results based upon restriction endonuclease analysis and viral protein patterns obtained earlier. The main antigenic differences of representative virus strains were found on two glycoproteins designated 3 and 12. Caprine herpesvirus 1, included in this study because of its serological relationship to BHV-1, differed fundamentally from BHV-1 on the grounds of McAb reactivity.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000914:Antibodies, Viral / Q000276:immunology*; D002417:Cattle; D006041:Goats; D006564:Herpesviridae / Q000276:immunology; D006566:Herpesviridae Infections / Q000382:microbiology / Q000662:veterinary; D007242:Herpesvirus 1, Bovine / Q000145:classification / Q000276:immunology* / Q000302:isolation & purification; D007158:Immunologic Techniques; D009422:Nervous System Diseases / Q000382:microbiology / Q000662:veterinary; D014764:Viral Proteins / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D014764:Viral Proteins