[Genetic characteristics and physical organization of the R-plasmid pBS52 with a broad range of bacterial hosts].
The genetic and physical data on Pseudomonas aeruginosa plasmid pBS52 coding for the resistances to ampicillin, streptomycin and sulfonamids have been obtained. This conjugative plasmid is transferable to a broad range of gram-negative bacterial hosts and compatible with the broad host-range plasmids from all known incompatibility groups. The plasmid size has been determined (38 Kb) and a physical map has been constructed using restriction endonucleases EcoRI, EcoRV, BamHI, BglII, PstI, PvuII, SalI, SlaI. The presence of a fragment, approximately 200 bp in size, which contains the sites for many of widely used restriction endonucleases is a characteristic feature of the plasmid pBS52.
D001435:Bacteriophages / Q000235:genetics; D003227:Conjugation, Genetic; D004262:DNA Restriction Enzymes; D011550:Pseudomonas aeruginosa / Q000235:genetics*; D011815:R Factors
D004262:DNA Restriction Enzymes
Geneticheskaia kharakteristika i fizicheskaia organizatsiia R-plazmidypBS52 s shirokim krugom bakterial'nykh khoziaev.