title: cDNA clone encoding a high molecular weight antigen of Babesia bovis.
abstract: An expression library was constructed by inserting cDNA copied from mRNA of the blood stages of Babesia bovis isolate KA into bacteriophage lambda gt11-amp3. An antigen-positive cDNA clone detected by screening the library with antibodies from cattle vaccinated with the KA isolate was shown to encode part of a high-molecular weight polypeptide antigen of B. bovis. This molecule was a dominant immunogen and was found by immunofluorescence to be within the parasite in infected erythrocytes.
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000032:analysis / Q000235:genetics*; D001403:Babesia / Q000235:genetics* / Q000276:immunology; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D004262:DNA Restriction Enzymes; D004251:DNA Transposable Elements; D015246:Deoxyribonuclease EcoRI; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000469:parasitology; D007118:Immunoassay; D009693:Nucleic Acid Hybridization; D010455:Peptides / Q000032:analysis / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D000953:Antigens, Protozoan; D004251:DNA Transposable Elements; D010455:Peptides; D012333:RNA, Messenger; D004247:DNA; D004262:DNA Restriction Enzymes; D015246:Deoxyribonuclease EcoRI