title: A temperature-sensitive mutant of Abelson murine leukemia virus confers inducibility of IgM expression to transformed lymphoid cells.
abstract: Lymphoid cell lines were isolated that were inducible for the expression of surface immunoglobulin by shift from 35.5 to 39.5 degrees C after infection of mouse bone marrow cells with a mutagen-treated Abelson murine leukemia virus. Virus produced by one of the cell lines (ts49) transmitted the temperature-sensitive phenotype to new lymphoid transformants as well as to NIH/3T3 cells. In addition, the tyrosine autophosphorylating activity of the p120gag-abl protein synthesized in ts49-transformed cells was found to be temperature-sensitive. Shift experiments using ts49-transformed lymphoid cells showed that at 39.5 degrees C they synthesize increased amounts of mu and kappa chain RNA and protein, and that they can be further induced to secrete IgM when treated with lipopolysaccharide.
mesh_terms: D000011:Abelson murine leukemia virus / Q000235:genetics* / Q000276:immunology; D000818:Animals; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D005796:Genes; D005814:Genes, Viral; D006412:Hematopoietic Stem Cells / Q000276:immunology; D007075:Immunoglobulin M / Q000096:biosynthesis / Q000235:genetics*; D009052:Leukemia Virus, Murine / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D009154:Mutation; D011947:Receptors, Antigen, B-Cell / Q000235:genetics; D013696:Temperature
chemical_list: D007075:Immunoglobulin M; D011947:Receptors, Antigen, B-Cell