[Properties of the plasmid pFT15/10-1 isolated from the vaccine strain of Francisella tularensis].
Plasmid, designated pFT15/10-1, was isolated from Francisella tularensis vaccine strain 15/10. The plasmid is presented by the homogeneous 5.02 +/- 0.054 Md monomeric circular DNA molecules in electron microscopic preparations. Plasmid size is 7-7.3 kb as defined by electrophoresis in agarose gel. The restriction analysis has revealed that plasmid pFT15/10-1 possesses a single specific cleavage site for restriction endonuclease EcoRI, two sites for restriction endonucleases BamHI, BgIII, HincII, HindIII, PstI, three sites for BglI and SalI, some for AluI, TagI, MvaI, CfrI. Plasmid is not digested by restriction endonucleases SmaI, XmaI, KpnI, MluI. Restriction map of the plasmid was constructed for most frequently used restriction endonucleases.
D001428:Bacterial Vaccines; D004262:DNA Restriction Enzymes; D004352:Drug Resistance, Microbial; D005604:Francisella tularensis / Q000235:genetics* / Q000276:immunology; D005819:Genetic Markers; D010957:Plasmids
D001428:Bacterial Vaccines; D005819:Genetic Markers; D004262:DNA Restriction Enzymes
Svoĭstva plazmidy pFT15/10-1, vydelennoĭ iz vaktsinnogo shtamma Francisella tularensis.