[Molecular cloning of provirus sequences of Rauscher leukemia virus from mouse erythroleukemia cell genome].
Provirus from a component of Rauscher leukaemia virus (RLV) has been cloned. The provirus (the size of 5000 b. p.) contains two LTR sequences and shares expressed sequence homology with Mo-MuLV. Restriction analysis and determination of the LTR nucleotide sequence and of the site from 3'-end of proviral genome have shown the cloned provirus to be the SFEV component of RLV. LTR from this cloned provirus contains all sites necessary for transcription: CAAT and TATA sequences, "cap" site and polyadenylation signal. The LTR of the cloned provirus from SFEV component of RLV has been shown to function as a promoter in E. coli cells.
D000818:Animals; D001483:Base Sequence; D002471:Cell Transformation, Neoplastic; D002472:Cell Transformation, Viral; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000235:genetics*; D004900:Erythroblasts / Q000382:microbiology; D004915:Leukemia, Erythroblastic, Acute / Q000235:genetics* / Q000382:microbiology; D051379:Mice; D011925:Rauscher Virus / Q000235:genetics*
D004279:DNA, Viral; D004262:DNA Restriction Enzymes
Molekuliarnoe klonirovanie provirusnykh posledovatel'nosteĭ leĭkoza Raushera iz genoma myshinykh éritroleĭkoznykh kletok.