Vitamins E and K induce aryl hydrocarbon hydroxylase activity in human cell cultures.
Two fat soluble vitamins, Vitamins E and K, when added into culture medium, were found to increase aryl hydrocarbon hydroxylase activity in human cultured cells. The extent of induction in a hepatoma-derived cell line (Hep G2) by these vitamins is of similar magnitude to those cells receiving benz[a]anthracene; whereas in a mammary tumor-derived cell line (MCF-7), benz[a]anthracene is the best inducer for the hydroxylase activity. The increase of the hydroxylase activity is associated with increased levels of a specific mRNA coding for polynuclear aromatic hydrocarbons-induced form of cytochrome P-450 with Vitamins E and K treatment. The size of the induced mRNA is 3.3 kilobase which is the same as that of benz[a]anthracene treatment.
D001189:Aryl Hydrocarbon Hydroxylases / Q000096:biosynthesis*; D001943:Breast Neoplasms / Q000201:enzymology; D006528:Carcinoma, Hepatocellular / Q000201:enzymology; D002460:Cell Line; D004790:Enzyme Induction / Q000187:drug effects; D006801:Humans; D007700:Kinetics; D008113:Liver Neoplasms; D014810:Vitamin E / Q000494:pharmacology*; D014812:Vitamin K / Q000494:pharmacology*
D014812:Vitamin K; D014810:Vitamin E; D001189:Aryl Hydrocarbon Hydroxylases