Serial propagation of porcine group C rotavirus (pararotavirus) in primary porcine kidney cell cultures.
A porcine group C rotavirus was adapted to serial propagation in roller tube cultures of primary porcine kidney cells with high concentrations of pancreatin. Infected cells were identified by immunofluorescence staining of cell monolayers. Only group C rotavirus particles were observed in culture supernatants by immune electron microscopy.
D000818:Animals; D002478:Cells, Cultured; D005455:Fluorescent Antibody Technique; D007668:Kidney; D008854:Microscopy, Electron; D010194:Pancreatin; D012401:Rotavirus / Q000254:growth & development*; D013552:Swine; D014776:Virus Cultivation
D010194:Pancreatin