Hypogravity-induced atrophy of rat soleus and extensor digitorum longus muscles.
Prolonged exposure of humans to hypogravity causes weakening of their skeletal muscles. This problem was studied in rats exposed to hypogravity for 7 days aboard Spacelab 3. Hindlimb muscles were harvested 12-16 hours postflight for histochemical, biochemical, and ultrastructural analyses. The majority of the soleus and extensor digitorum longus fibers exhibited simple cell shrinkage. However, approximately 1% of the fibers in flight soleus muscles appeared necrotic. Flight muscle fibers showed increased glycogen, lower subsarcolemmal staining for mitochondrial enzymes, and fewer subsarcolemmal mitochondria. During atrophy, myofibrils were eroded by multiple focal losses of myofilaments; lysosomal autophagy was not evident. Tripeptidylaminopeptidase and calcium-activated protease activities of flight soleus fibers were significantly increased, implying a role in myofibril breakdown. Simple fiber atrophy appears to account for muscle weakening during spaceflight, but fiber necrosis is also a contributing factor.
D000626:Aminopeptidases / Q000378:metabolism; D000818:Animals; D002154:Calpain / Q000378:metabolism; D008297:Male; D009132:Muscles / Q000378:metabolism / Q000473:pathology; D009133:Muscular Atrophy / Q000209:etiology* / Q000378:metabolism / Q000473:pathology; D009210:Myofibrils / Q000648:ultrastructure; D009245:NADH Dehydrogenase / Q000378:metabolism; D051381:Rats; D013026:Space Flight; D014893:Weightlessness / Q000009:adverse effects*
D009245:NADH Dehydrogenase; D000626:Aminopeptidases; D002154:Calpain