Synthesis and biological characterization of monocyte-derived neutrophil chemotactic factor.
MDNCF is a human monocyte-derived, 72-residue chemotactic peptide, which has sequence similarity with members of a family of pro-inflammatory cytokines. The peptide was synthesized by the solid-phase method, and is identical to the natural peptide in amino acid composition, sequence and chemotactic potency. MDNCF forms two loops via a neighboring pair of disulfide bridges, the probable locations of which are residues 7-34 and 9-50. Reduction and alkylation eliminated chemotactic activity. MDNCF fragments 7-37, 30-72 and 17-72 were all biologically inactive. The data suggest that the region of the clustered pair of disulfide bridges is important for biological activity.
D000478:Alkylation; D001681:Biological Assay; D001688:Biological Products / Q000138:chemical synthesis*; D002630:Chemotactic Factors / Q000138:chemical synthesis*; D002634:Chemotaxis, Leukocyte; D006801:Humans; D016209:Interleukin-8; D015846:Monokines; D009504:Neutrophils / Q000502:physiology; D010084:Oxidation-Reduction; D011487:Protein Conformation; D013329:Structure-Activity Relationship
D001688:Biological Products; D002630:Chemotactic Factors; D016209:Interleukin-8; D015846:Monokines