Development of donor-derived thymic lymphomas after allogeneic bone marrow transplantation in AKR/J mice.
The transplantation of bone marrow cells from BALB/c (but not C57BL/6 and C3H/HeN) mice was observed to lead to the development of thymic lymphomas (leukemias) in AKR/J mice. Two leukemic cell lines, CAK1.3 and CAK4.4, were established from the primary culture of two thymic lymphoma, and surface phenotypes of these cell lines found to be H-2d and Thy-1.2+, indicating that these lymphoma cells are derived from BALB/c donor bone marrow cells. Further analyses of surface markers revealed that CAK1.3 is L3T4+ Lyt2+ IL2R-, whereas CAK4.4 is L3T4- Lyt2- IL2R+. Both CAK1.3 and CAK4.4 were transplantable into BALB/c but not AKR/J mice, further indicating that these cells are of BALB/c bone marrow donor origin. The cells were found to produce XC+-ecotropic viruses, but xenotropic and mink cell focus-forming viruses were undetectable. Inasmuch as thymic lymphomas are derived from bone marrow cells of leukemia-resistant BALB/c strain of mice under the allogeneic environment of leukemia-prone AKR/J mice, this animal model may serve as a useful tool not only for the analysis of leukemic relapse after bone marrow transplantation but also for elucidation of the mechanism of leukemogenesis.
D000818:Animals; D000954:Antigens, Surface; D016026:Bone Marrow Transplantation; D002460:Cell Line; D005260:Female; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000209:etiology* / Q000382:microbiology / Q000473:pathology; D051379:Mice; D008806:Mice, Inbred AKR / Q000276:immunology*; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D010641:Phenotype; D011828:Radiation Chimera; D012190:Retroviridae / Q000254:growth & development; D013045:Species Specificity; D013601:T-Lymphocytes / Q000145:classification / Q000382:microbiology; D013953:Thymus Neoplasms / Q000209:etiology* / Q000382:microbiology / Q000473:pathology; D014019:Tissue Donors
D000954:Antigens, Surface