A preliminary evaluation of five HIV antigen detection assays.
Five commercial assays for detecting HIV antigen were evaluated using a panel of 40 coded samples in six laboratories. All assays were capable of detecting HIV-1 antigen (HIV-1 Ag), and are likely to prove useful for monitoring supernatant fluids from a variety of cell cultures. The concentration of HIV-1 protein which the assays detected varied from 25 ng/ml down to 25 pg/ml. Three of the five assays were also able to detect HIV-2 Ag. More extensive evaluations are needed to determine the sensitivity and specificity of these assays on serum samples and body fluids.
D000914:Antibodies, Viral / Q000276:immunology; D000956:Antigens, Viral / Q000032:analysis*; D002478:Cells, Cultured; D006678:HIV / Q000276:immunology / Q000302:isolation & purification*; D015483:HIV Antibodies; D006801:Humans; D007124:Immunoenzyme Techniques; D008214:Lymphocytes / Q000382:microbiology; D011863:Radioimmunoassay / Q000379:methods; D014764:Viral Proteins / Q000032:analysis*
D000914:Antibodies, Viral; D000956:Antigens, Viral; D015483:HIV Antibodies; D014764:Viral Proteins