Effect on leukocyte locomotion and superoxide production by uremic toxins and polyamines.
We have isolated peak II, which is the peak of the middle molecules containing polyamines, from dialysate of uremic patients in recirculating dialysis. We investigated the effect of total dialysate, chromatographic peaks, I, II, III, IV and commercial polyamines on polymorphonuclear leukocyte chemotaxis and superoxide production (i.e. phagocytic activity) in healthy and uremic patients. Polymorphonuclear chemotaxis was inhibited by total dialysate and peak II but not by polyamines; polyamines, total dialysate and peak II had no activity on polymorphonuclear phagocytosis.
D002465:Cell Movement; D002634:Chemotaxis, Leukocyte; D006801:Humans; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology*; D010587:Phagocytosis; D011073:Polyamines / Q000494:pharmacology*; D006435:Renal Dialysis; D013481:Superoxides / Q000096:biosynthesis*; D014118:Toxins, Biological; D014511:Uremia / Q000503:physiopathology*
D011073:Polyamines; D014118:Toxins, Biological; D013481:Superoxides