title: In vitro methylation of the 5'-flanking regions of the mouse beta-globin gene.
abstract: The enzymatic methylation of the 5'-flanking region of the mouse beta-globin (major) gene containing putative regulatory regions has been investigated. In vitro methylation of this 368-base pair regulatory DNA by a DNA methyltransferase obtained from mouse erythroleukemia cells yields an asymmetric methylation pattern. Of the 10 available CG pairs, only 5-6 are modified, leading to one hemimethylated site and two apparently fully methylated sites. Only CG pairs which are localized in a 29-base pair cluster are methylated. The data suggest that a CG cluster approximately 100 base pairs upstream from the CAP site may be the in vivo site of methylation in the 5'-regulator region of the mouse beta-globin gene.
mesh_terms: D000818:Animals; D001482:Base Composition; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism*; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000378:metabolism*; D004262:DNA Restriction Enzymes; D005914:Globins / Q000235:genetics*; D004915:Leukemia, Erythroblastic, Acute / Q000201:enzymology; D007942:Leukemia, Experimental / Q000201:enzymology; D008745:Methylation; D051379:Mice; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D012333:RNA, Messenger; D005914:Globins; D004247:DNA; D004248:DNA (Cytosine-5-)-Methyltransferases; D004262:DNA Restriction Enzymes