title: Reconstitution of pyrroloquinoline quinone-dependent D-glucose oxidase respiratory chain of Escherichia coli with cytochrome o oxidase.
abstract: D-Glucose dehydrogenase is a pyrroloquinoline quinone-dependent primary dehydrogenase linked to the respiratory chain of a wide variety of bacteria. The enzyme exists in the membranes of Escherichia coli, mainly as an apoenzyme which can be activated by the addition of pyrroloquinoline quinone and magnesium. Thus, membrane vesicles of E. coli can oxidize D-glucose to gluconate and generate an electrochemical proton gradient in the presence of pyrroloquinoline quinone. The D-glucose oxidase-respiratory chain was reconstituted into proteoliposomes, which consisted of two proteins purified from E. coli membranes, D-glucose dehydrogenase and cytochrome o oxidase, and E. coli phospholipids containing ubiquinone 8. The electron transfer rate during D-glucose oxidation and the membrane potential generation in the reconstituted proteoliposomes were almost the same as those observed in the membrane vesicles when pyrroloquinoline quinone was added. The results demonstrate that the quinoprotein, D-glucose dehydrogenase, can reduce ubiquinone 8 directly within phospholipid bilayer and that the D-glucose oxidase system of E. coli has a relatively simple respiratory chain consisting of primary dehydrogenase, ubiquinone 8, and a terminal oxidase.
mesh_terms: D003576:Electron Transport Complex IV / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology*; D005949:Glucose Oxidase / Q000378:metabolism*; D006899:Mixed Function Oxygenases / Q000378:metabolism; D010101:Oxygen Consumption; D045542:PQQ Cofactor; D011510:Proteolipids / Q000378:metabolism; D011804:Quinolines / Q000378:metabolism*
chemical_list: D011510:Proteolipids; D011804:Quinolines; C020485:proteoliposomes; D045542:PQQ Cofactor; D006899:Mixed Function Oxygenases; D005949:Glucose Oxidase; C021497:lactate 2-monooxygenase; C038862:cytochrome o oxidase; D003576:Electron Transport Complex IV