title: Effect of internal viral sequences on the utility of retroviral vectors.
abstract: Expression of the human ADA cDNA encoded by the Moloney murine leukemia virus spliced RNA form is enhanced by intron-contained sequences. The presence of sequences corresponding to the viral gag gene in a Moloney murine leukemia virus-based vector results in the generation of 10- to 40-fold higher titers of virus.
mesh_terms: D000243:Adenosine Deaminase / Q000235:genetics; D005822:Genetic Vectors; D019868:Kanamycin Kinase; D009052:Leukemia Virus, Murine / Q000235:genetics; D010770:Phosphotransferases / Q000235:genetics; D012326:RNA Splicing; D012091:Repetitive Sequences, Nucleic Acid; D012190:Retroviridae / Q000235:genetics*; D012641:Selection, Genetic
chemical_list: D010770:Phosphotransferases; D019868:Kanamycin Kinase; D000243:Adenosine Deaminase