Carcinogenicity testing in nude mouse cell cultures.
Secondary cultures of newborn NMRI nu/nu (nude) mouse skin fibroblasts were used as targets for transformation by the combined administration of SV40 and 3-methylcholanthrene (MC). The long (72 h) post-treatment with MC increased virus transformation as much as 4.3-fold. In contrast, anthracene, a non-carcinogenic compound, had no effect on viral transformation frequency. Despite considerable variation within a group, the cell lines transformed by the combination treatment, as a group, were more tumourigenic than cell lines transformed by SV40 alone.
D000818:Animals; D002471:Cell Transformation, Neoplastic; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D008748:Methylcholanthrene / Q000633:toxicity; D051379:Mice; D008819:Mice, Nude; D009152:Mutagenicity Tests / Q000379:methods*; D013539:Simian virus 40
D008748:Methylcholanthrene