title: The use of permanganate as a sequencing reagent for identification of 5-methylcytosine residues in DNA.
abstract: The use of permanganate as a reagent for DNA sequencing by chemical degradation has been studied with respect to its specificity for 5-methylcytosine residues. At weakly acidic pH and room temperature, 0.2 mM potassium permanganate reacts preferentially with thymine, 5-methylcytosine, and to a lesser extent with purine residues, while cytosine remains essentially intact. Permanganate oxidation is, therefore, a suitable DNA sequencing reaction for positive discrimination between 5-methylcytosine and unmethylated cytosine.
mesh_terms: D044503:5-Methylcytosine; D001483:Base Sequence; D003596:Cytosine / Q000031:analogs & derivatives* / Q000032:analysis; D004247:DNA; D004262:DNA Restriction Enzymes; D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides; D010084:Oxidation-Reduction; D011196:Potassium Permanganate
chemical_list: D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides; D011196:Potassium Permanganate; D044503:5-Methylcytosine; D003596:Cytosine; D004247:DNA; D004262:DNA Restriction Enzymes