Variables affecting resolution of lung phospholipids in one-dimensional thin-layer chromatography.
Resolution of the confusion in the literature about the separation of lung phospholipids in thin-layer chromatographic systems has awaited a systematic study of the variables that potentially affect this separation. In this study I show that: incorporation of ammonium sulfate into silica gel "GHL" has a dramatic effect on separation of lung phospholipids; this effect is equally dramatic but different in activated and nonactivated gels; when it picks up moisture, ammonium sulfate-activated gel very rapidly loses its ability to resolve lecithin from phosphatidylinositol; in gel containing ammonium sulfate, small amounts of phosphatidylethanolamine are hydrolyzed to lyso-phosphatidylethanolamine.
D000645:Ammonium Sulfate; D000653:Amniotic Fluid / Q000032:analysis; D002855:Chromatography, Thin Layer / Q000379:methods; D005325:Fetal Organ Maturity; D006801:Humans; D006813:Humidity; D008168:Lung / Q000196:embryology*; D010743:Phospholipids / Q000032:analysis*; D058428:Silica Gel; D012822:Silicon Dioxide
D010743:Phospholipids; D058428:Silica Gel; D012822:Silicon Dioxide; D000645:Ammonium Sulfate