Interaction of met-enkephalin with human granulocytes.
Met-enkephalin has been found to have an effect on cell shape and motility of human polymorphonuclear leukocytes (PMNs). Specific binding of tritium-labeled Met-enkephalin to the cells could not be demonstrated. There was a rapid proteolytic degradation of the peptide in the medium, followed by uptake of the labeled tyrosine. The peptidase recognizes the N-terminal sequence of various endogenous opioid peptides. The protease inhibitors bestatin and bacitracin had but little effect on the degradation of Met-Enk.
D002465:Cell Movement / Q000187:drug effects; D004744:Enkephalin, Methionine / Q000097:blood / Q000494:pharmacology*; D004745:Enkephalins / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D011957:Receptors, Opioid / Q000032:analysis
D004745:Enkephalins; D011957:Receptors, Opioid; C033593:methionine-enkephalin receptor; D004744:Enkephalin, Methionine