An automated solid-phase 17 alpha-hydroxyprogesterone ELISA method using a microtitre plate.
We report an automated ELISA method for the measurement of 17 alpha-hydroxyprogesterone in plasma samples. A rabbit antiserum raised against 17 alpha-hydroxyprogesterone-3-(O-carboxymethyl)oxime-bovine serum albumin is used for the assay and a homologous competitor 17 alpha-hydroxyprogesterone-3-(O-carboxymethyl)oxime-bovine thyroglobulin is coated onto a microtitre plate. A goat anti-rabbit IgG-horse radish peroxidase is used as a probe for this solid-phase assay. The assay exhibits good sensitivity, precision and accuracy. The method is used routinely for the management of patients with congenital adrenal hyperplasia.
D019326:17-alpha-Hydroxyprogesterone; D002021:Buffers; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D006868:Hydrolysis; D006908:Hydroxyprogesterones / Q000097:blood*; D007457:Iodine Radioisotopes; D010447:Peptide Hydrolases; D011863:Radioimmunoassay
D002021:Buffers; D006908:Hydroxyprogesterones; D007457:Iodine Radioisotopes; D019326:17-alpha-Hydroxyprogesterone; D010447:Peptide Hydrolases