Expression of human atrial natriuretic polypeptide gene in Cos 7 cells.
Cos 7 cells transfected with human atrial natriuretic polypeptide (hANP) gene with SV40 enhancer and replication origin sequences expressed hANP gene. The expressed RNA was indistinguishable from native hANP mRNA and the transcribed protein seemed to be properly processed to alpha-hANP and beta-hANP. This system provides a useful approach to investigate the processing of hANPs and the structure-function relationship of amino acid sequences of hANPs.
D000818:Animals; D009320:Atrial Natriuretic Factor / Q000235:genetics* / Q000302:isolation & purification; D002460:Cell Line; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004742:Enhancer Elements, Genetic; D005796:Genes; D006801:Humans; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D013539:Simian virus 40 / Q000235:genetics; D014158:Transcription, Genetic
D009320:Atrial Natriuretic Factor; D004262:DNA Restriction Enzymes