Genetic characterization of JC virus Tokyo-1 strain, a variant oncogenic in rodents.
The genome DNA of JC virus Tokyo-1 strain [JCV(Tokyo-1)], a variant oncogenic in rodents, was molecularly cloned directly from the brain of a Japanese patient with progressive multifocal leukoencephalopathy and from tissue culture, and the restriction enzyme cleavage pattern and regulatory sequences were determined. The restriction pattern of the cloned JCV(Tokyo-1) DNA was different from those of JCVs previously reported in the United States and Germany. Also, the arrangement of the regulatory sequence was unique to this strain. Thus JCV(Tokyo-1) can be classified as a new subtype. The relationship between the restriction pattern and the regulatory sequence of JCV(Tokyo-1), and its characteristic oncogenicity, is discussed.
D000818:Animals; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000032:analysis*; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005786:Gene Expression Regulation; D005814:Genes, Viral; D006801:Humans; D007577:JC Virus / Q000235:genetics* / Q000472:pathogenicity; D009374:Neoplasms, Experimental / Q000209:etiology; D011120:Polyomavirus / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D012377:Rodentia
D004279:DNA, Viral; D004262:DNA Restriction Enzymes