Feasibility study of imaging a living murine tumor by electron paramagnetic resonance.
An electron paramagnetic resonance image was measured for the first time from in vivo field gradient spectra of a living murine tumor (Cloudman S-91 melanoma in the tail of a DBA-2J mouse) using the paramagnetic nitroxide imaging agent 3-carboxamido-2,2,5,5-tetramethylpyrroline-1-oxyl injected into the tail vein. The experiments were accomplished at L-band frequency (1.55 GHz) with a single-turn flat-loop coil. A cross-sectional image was obtained perpendicular to the tail axis, which clearly distinguished features to the submillimeter resolution level.
D000818:Animals; D003497:Cyclic N-Oxides; D004578:Electron Spin Resonance Spectroscopy; D008545:Melanoma / Q000175:diagnosis; D051379:Mice; D008811:Mice, Inbred DBA; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000175:diagnosis*; D013113:Spin Labels
D003497:Cyclic N-Oxides; D013113:Spin Labels; C047253:3-carbamoyl-2,2,5,5-tetramethyl-3-pyrroline-1-yloxyl