title: Effects of phospholipases C from bacteria on binding of enkephalin to rat brain membranes.
abstract: The effects of phospholipases C on the equilibrium constants and maximal binding capacities of tritiated [D-Ala2,-D-Leu5] enkephalin to rat brain membranes were investigated using phosphatidylcholine-hydrolyzing phospholipase C and sphingomyelinase C of Bacillus cereus and, phosphatidylinositol-specific phospholipase C of Bacillus thuringiensis. When 72% of the phosphatidylinositol in the rat brain membranes was hydrolyzed by phosphatidylinositol-specific phospholipase C, the affinity of opiate receptor for [D-Ala2,-D-Leu5] enkephalin was almost doubled and maximal binding of [D-Ala2,-D-Leu5] enkephalin was decreased to 87% of control. Although specific [D-Ala2,-D-Leu5] enkephalin binding was decreased with phosphatidylinositol hydrolysis when measured at higher concentration (30 nM) of [D-Ala2,-D-Leu5] enkephalin, the specific binding was increased with the hydrolysis of phosphatidylinositol when measured at lower concentration (6 nM) of the ligand. On treatment of membranes with phosphatidylcholine-hydrolyzing phospholipase C, specific [D-Ala2,-D-Leu5] enkephalin binding was drastically decreased with the progressive hydrolysis of phosphatidylcholine in the rat brain membranes, and specific binding was completely lost after 81% hydrolysis of phosphatidylcholine. However, the affinity of opiate receptor for [D-Ala2,-D-Leu5] enkephalin was not influenced, and maximal binding was decreased to 32% of the control when 61% of phosphatidylcholine was hydrolyzed. Treatment with sphingomyelinase C did not cause any appreciable reduction of specific [D-Ala2,-D-Leu5] enkephalin binding. From these results, it is concluded that the binding of [D-Ala2,-D-Leu5] enkephalin to opiate receptor is influenced by changes in the phospholipid environment of the rat brain membranes, and that phosphatidylinositol may be a modulator for the function of the receptor.
mesh_terms: D000818:Animals; D001409:Bacillus cereus / Q000201:enzymology; D001413:Bacillus thuringiensis / Q000201:enzymology; D001921:Brain / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D004745:Enkephalins / Q000378:metabolism*; D066298:In Vitro Techniques; D008563:Membrane Lipids / Q000502:physiology*; D010743:Phospholipids / Q000502:physiology*; D051381:Rats; D011957:Receptors, Opioid / Q000378:metabolism*; D010738:Type C Phospholipases / Q000378:metabolism*
chemical_list: D004745:Enkephalins; D008563:Membrane Lipids; D010743:Phospholipids; D011957:Receptors, Opioid; D010738:Type C Phospholipases