title: The influence of dolichols on fluidity of mouse synaptic plasma membranes.
abstract: Dolichols are isoprenologues which constitute an important component of biological membranes. However, an understanding of the effects of dolichols on the organization and dynamics of biological membranes has not been forthcoming. The experiments reported here are aimed at understanding the effects of dolichols on the physical properties of mouse brain synaptic plasma membranes. The effect of dolichols incorporated into mouse brain synaptic plasma membranes on fluorescent and electron spin resonance probes sensing the hydrophobic core differed from that of probes reporting closer to the surface of membrane bilayers. Dolichols significantly (P less than 0.01) lowered the polarization, limiting anisotropy, and order parameter of diphenylhexatriene in synaptic plasma membranes and liposomes extracted from synaptic plasma membranes, without changing the rotational relaxation time. Similarly, dolichol increased the fluidity reported by 16-doxylstearic acid in synaptic plasma membranes or liposomes extracted from synaptic plasma membranes. In contrast, dolichols exerted no effect on those properties for trans-parinaric acid or 5-doxylstearic acid in synaptic plasma membranes or liposomes derived therefrom. Dolichols can dramatically alter the structure and dynamics of lipid motion in synaptic plasma membranes and these effects are dependent on the location of the probe in the membrane.
mesh_terms: D000818:Animals; D001921:Brain / Q000502:physiology / Q000648:ultrastructure; D004161:Diphenylhexatriene / Q000494:pharmacology; D004224:Diterpenes / Q000494:pharmacology*; D004286:Dolichols / Q000494:pharmacology*; D004578:Electron Spin Resonance Spectroscopy; D005454:Fluorescence Polarization; D008560:Membrane Fluidity / Q000187:drug effects*; D051379:Mice; D013229:Stearic Acids; D013570:Synaptic Membranes / Q000502:physiology*; D013574:Synaptosomes; D013696:Temperature
chemical_list: D004224:Diterpenes; D004286:Dolichols; D013229:Stearic Acids; D004161:Diphenylhexatriene