title: Physical analysis of Tn10- and IS10-promoted transpositions and rearrangements.
abstract: We have investigated by Southern blot hybridization the rate of IS10 transposition and other Tn10/IS10-promoted rearrangements in Escherichia coli and Salmonella strains bearing single chromosomal insertions of Tn10 or a related Tn10 derivative. We present evidence for three primary conclusions. First, the rate of IS10 transposition is approximately 10(-4) per cell per bacterial generation when overnight cultures are grown and plated on minimal media and is at least ten times more frequent than any other Tn10/IS10-promoted DNA alteration. Second, all of the chromosomal rearrangements observed can be accounted for by two previously characterized Tn10-promoted rearrangements: deletion/inversions and deletions. Together these rearrangements occur at about 10% the rate of IS10 transposition. Third, the data suggest that intramolecular Tn10-promoted rearrangements preferentially use nearby target sites, while the target sites for IS10 transposition events are scattered randomly around the chromosome.
mesh_terms: D002872:Chromosome Deletion; D007446:Chromosome Inversion; D003470:Culture Media; D004251:DNA Transposable Elements; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000235:genetics*; D005690:Galactose / Q000235:genetics; D006639:Histidine / Q000235:genetics; D011995:Recombination, Genetic; D012475:Salmonella / Q000235:genetics*; D012641:Selection, Genetic; D013752:Tetracycline
chemical_list: D003470:Culture Media; D004251:DNA Transposable Elements; D006639:Histidine; D013752:Tetracycline; D005690:Galactose