[Mutagenesis on cloned yeast genes. The mutation of the yeast gene comprising the plasmid and chromosome].
The cells of Saccharomyces cerevisiae were transformed by plasmid pYG-007 treated in vitro with o-methylhydroxylamine. The plasmid consists of a portion of the bacterial plasmid with genes of resistance to ampicillin, chloramphenicol and tetracycline, 2 mkm yeast DNA and yeast genes ADE2 and LEU2. The collection of mutants containing a mutant allele of ADE2 gene within the plasmid was obtained. Interallelic complementation and that induced by suppression were studied in these ade 2 mutants. It was shown that all these induced ade 2 mutations were base-pair substitutions. Using the mechanism of conversion we managed to transfer the plasmid ade 2 mutations into the chromosome. Three pairs of strains carrying similar mutation in plasmid and chromosome were created. Analysis of frequency of reversions induced by UV-light and hydroxylaminopurine in the mutant ade2 locus comprised in the plasmid and chromosome showed that the former induced reversions in plasmid alleles less effectively than the latter.
D000483:Alleles; D002875:Chromosomes / Q000187:drug effects / Q000528:radiation effects; D003001:Cloning, Molecular / Q000187:drug effects / Q000528:radiation effects; D004269:DNA, Bacterial / Q000187:drug effects / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics; D005800:Genes, Fungal / Q000187:drug effects / Q000528:radiation effects; D006898:Hydroxylamines / Q000494:pharmacology; D009154:Mutation; D010957:Plasmids / Q000187:drug effects / Q000528:radiation effects; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics* / Q000528:radiation effects; D014170:Transformation, Genetic / Q000187:drug effects / Q000528:radiation effects; D014466:Ultraviolet Rays
D004269:DNA, Bacterial; D006898:Hydroxylamines; C005214:methoxyamine
Mutagenez na klonirovannykh genakh drozhzheĭ. Mutirovanie gena drozhzheĭ v sostave plazmidy i khromosomy.