Studies towards the development of chemically synthesized non-radioactive biotinylated nucleic acid hybridization probes.
Non-radioactive nucleic acid hybridization probes have been constructed in which the reporter group is long chain biotin chemically linked to a basic macromolecule (histone H1, cytochrome C or polyethyleneimine). The modified basic macromolecule which carries many biotin residues can, in turn, be covalently linked to nucleic acids (DNA) via the bifunctional cross-linking reagents, glutaraldehyde, 1,2,7,8-diepoxyoctane, bis (succinimidyl) suberate or bis (sulfonosuccinimidyl) suberate. This provides a very sensitive probe by which as little as between 10-50fg of target DNA can be visualized using dot-blot hybridization procedures in conjunction with avidin or streptavidin enzyme conjugates.
D001710:Biotin; D003124:Colorimetry; D003432:Cross-Linking Reagents; D003574:Cytochrome c Group; D004247:DNA / Q000378:metabolism; D006657:Histones; D007202:Indicators and Reagents; D008722:Methods; D009693:Nucleic Acid Hybridization; D011094:Polyethyleneimine
D003432:Cross-Linking Reagents; D003574:Cytochrome c Group; D006657:Histones; D007202:Indicators and Reagents; D001710:Biotin; D011094:Polyethyleneimine; D004247:DNA