[Restriction analysis of the genome of swine abortion parvovirus and cloning of its PstI-EcoRI fragment].
The cultured pig kidney cells infected by the porcine parvovirus (PPV) produced the virions and viral DNA. The latter was used as a matrix to synthesize the double stranded DNA. The obtained preparation is more homogenic than the natural replicative form and was used for restriction analysis of porcine parvovirus genome and for molecular cloning of its fragment. The isolated recombinant plasmids contained the PstI-EcoRI fragment of PPV DNA, containing 70% of the viral genome. The restriction analysis of replicative PPV DNA isolated from the infected cells has resulted in finding of the replicative form containing a 300 bp deletion in the 5'-region of PPV genome.
D000034:Abortion, Veterinary / Q000382:microbiology; D000818:Animals; D001483:Base Sequence; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000235:genetics; D015246:Deoxyribonuclease EcoRI; D015252:Deoxyribonucleases, Type II Site-Specific; D005260:Female; D005814:Genes, Viral; D010321:Parvoviridae / Q000235:genetics*; D010957:Plasmids; D011247:Pregnancy; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology
D004279:DNA, Viral; D004262:DNA Restriction Enzymes; D015246:Deoxyribonuclease EcoRI; C024388:CTGCAG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific
Restriktsionnyĭ analiz genoma parvovirusa aborta svineĭ i klonirovanie ego PstI-EcoRI-fragmenta.