In vitro inhibition of lipopolysaccharide-induced bone resorption by polymyxin B.
Lipopolysaccharide (LPS) purified from Haemophilus actinomycetemcomitans stimulates resorption and inhibits collagen synthesis in mouse calvaria bones in vitro. Addition of polymyxin B caused a dose-related inhibition of LPS-stimulated bone resorption and reversal of the inhibition of collagen synthesis. A polymyxin B to LPS ratio of 2:1 prevented bone resorption and restored collagen synthesis to control levels. The activity of polymyxin B was specific for LPS as bone resorption induced by prostaglandin E2 or parathyroid hormone was unaffected at similar concentrations. These results indicate that polymyxin B and its analogues may have potential in the treatment of periodontal disease by reducing bone loss induced by lipopolysaccharides.
D000818:Animals; D001862:Bone Resorption / Q000187:drug effects*; D001842:Bone and Bones / Q000378:metabolism; D003094:Collagen / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D006190:Haemophilus; D066298:In Vitro Techniques; D008070:Lipopolysaccharides / Q000494:pharmacology*; D051379:Mice; D010510:Periodontal Diseases / Q000188:drug therapy; D011112:Polymyxin B / Q000494:pharmacology*; D011113:Polymyxins / Q000494:pharmacology*
D008070:Lipopolysaccharides; D011113:Polymyxins; D003094:Collagen; D011112:Polymyxin B