Phenotypic, cytogenetic and molecular characterization of a new B-chronic lymphocytic leukaemia (B-CLL) cell line.
A lymphoid cell line was established from a patient with B-cell chronic lymphocytic leukaemia (B-CLL) by infecting blood lymphocytes with Epstein-Barr virus (EBV). Immunoglobulin gene rearrangement studies and the presence of a chromosome marker (isochromosome 17q) provided the formal proof that the line has originated from the neoplastic B cells. The morphology and phenotype indicate that the EBV-induced cell line has reached a plasma cell-like stage of differentiation.
D001402:B-Lymphocytes; D002454:Cell Differentiation; D002460:Cell Line; D002472:Cell Transformation, Viral; D002886:Chromosomes, Human, Pair 17 / Q000648:ultrastructure; D005819:Genetic Markers; D004854:Herpesvirus 4, Human; D006801:Humans; D007136:Immunoglobulins / Q000235:genetics; D007945:Leukemia, Lymphoid / Q000235:genetics / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D010950:Plasma Cells / Q000473:pathology*
D005819:Genetic Markers; D007136:Immunoglobulins