Enhancement of tumorigenicity with morphological progression in a BALB/c preneoplastic outgrowth line.
Four newly established mammary hyperplastic outgrowth lines were analyzed for their tumorigenic, morphological, and ovarian hormone-dependent growth properties in BALB/cMed mice. The mammary outgrowth lines were designated DIM-1, DIM-2, DIM-3, and DIM-4 to indicate their origin from the mammary cell line COMMA-D. DIM-1, DIM-2, and DIM-3 were classical hyperplastic alveolar outgrowth lines that possessed high tumor-producing capabilities and rapidly evolved by transplant generation (TG) 6 into ovarian hormone-independent populations. DIM-3 was also characterized by extensive formation of dilated (cystic) alveoli. This characteristic did not correlate either positively or negatively with the tumor-producing capabilities of these lines. DIM-4 was a ductal outgrowth line that exhibited a progression in several biological properties. This line progressed morphologically from a ductal outgrowth to a mixed outgrowth of small ducts (ductules) with scattered alveoli and then in subsequent passages to a uniform alveolar outgrowth. Concurrent with these morphological changes, the tumor-producing capabilities of DIM-4 increased from low [40% with the time for 50% of the transplants to produce mammary tumors (TE50) greater than 12 mo] to high (71% with a TE50 of 7.1 mo). The ductal outgrowth line (TG 4) was totally dependent on ovarian hormones for growth; however, later passages (TG 8) of DIM-4 were only partially ovarian hormone dependent for growth and ovarian hormone independent for maintenance of alveolar morphology. In addition, the tumorigenic response to 7,12-dimethylbenz[a]anthracene (CAS: 57-97-6) was low in the ductal stage (TG 4) and high in the alveolar stage (TG 7). The results demonstrate an enhancement of tumorigenicity with morphological and biological progression in a preneoplastic mammary cell population and support the hypothesis that the cells at high risk for tumorigenesis in the BALB/c mammary gland appear to be the alveolar cell type.
D015127:9,10-Dimethyl-1,2-benzanthracene; D000818:Animals; D002460:Cell Line; D008325:Mammary Neoplasms, Experimental / Q000473:pathology*; D008324:Mammary Tumor Virus, Mouse; D051379:Mice; D008807:Mice, Inbred BALB C; D009368:Neoplasm Transplantation; D009376:Neoplasms, Hormone-Dependent / Q000473:pathology; D010052:Ovariectomy; D011230:Precancerous Conditions / Q000473:pathology*
D015127:9,10-Dimethyl-1,2-benzanthracene