Cloning and in vitro expression of the measles virus matrix gene.
A cDNA library was prepared from Vero cells infected with the Edmonston strain of measles virus. A number of viral specific cDNA clones were isolated and characterized by Northern blot hybridization analysis. A cDNA clone containing a 1500 base pair insert which hybridizes to a viral specific transcript of approximately 1500 nucleotides was subcloned into pSP64 and used as an in vitro transcription template. The resulting RNA transcript was translated in a cell-free system, giving rise to a polypeptide which comigrates on polyacrylamide gels with the authentic measles virus matrix protein and is immunoprecipitated with antisera specific for the matrix protein.
D000818:Animals; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D005796:Genes; D005814:Genes, Viral; D008459:Measles virus / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D014176:Protein Biosynthesis; D014158:Transcription, Genetic; D014709:Vero Cells; D014763:Viral Matrix Proteins; D014764:Viral Proteins / Q000235:genetics*
D014763:Viral Matrix Proteins; D014764:Viral Proteins; D004262:DNA Restriction Enzymes