Translational regulation in mouse hepatitis virus infection is not mediated by altered intracellular ion concentrations.
Infection of mouse L-2 fibroblasts with mouse hepatitis virus (MHV) results in strong inhibition of host cell protein synthesis. Since it has been suggested in other virus systems that translational control is modulated by changes in the intracellular ionic environment, we investigated the possible occurrence of similar changes during MHV infection. Membrane permeability to extracellular sodium ions was measured by culturing MHV-infected cells in the presence of 22Na+. Sodium influx into MHV-infected cells rose dramatically from 4 to 6 h post-infection. This influx correlated chronologically with the expression of MHV-mediated cell fusion. Cell fusion was blocked by the addition of a monoclonal antibody against the MHV E2 glycoprotein. This addition also resulted in a reduction in the normal influx of 22Na+, suggesting that E2 expression was responsible, directly or indirectly, for the increased permeability to sodium ions in infected cells. Cultures of MHV-infected cells were labelled with [35S]methionine in the presence of medium supplemented with sodium chloride at final concentrations ranging from 150 mM to 350 mM. Incorporation of radiolabel into proteins decreased with increasing NaCl concentration; however, the ratio of viral to cellular protein synthesis remained relatively constant. Similarly, alteration of intracellular Na+ and K+ levels by treatment of infected cells with ouabain had little effect on the pattern of viral/cellular protein synthesis. Using monoclonal anti-E2 antibody to inhibit Na+ influx, we demonstrated normal inhibition of host cell protein synthesis. We therefore conclude that MHV-induced shut-off of host translation is not mediated by changes in intracellular Na+ concentrations.
D000818:Animals; D002463:Cell Membrane Permeability; D002472:Cell Transformation, Viral / Q000187:drug effects; D003470:Culture Media; D007477:Ions; D007700:Kinetics; D007739:L Cells / Q000382:microbiology; D051379:Mice; D006517:Murine hepatitis virus / Q000235:genetics*; D010042:Ouabain / Q000494:pharmacology; D014176:Protein Biosynthesis; D012964:Sodium / Q000378:metabolism
D003470:Culture Media; D007477:Ions; D010042:Ouabain; D012964:Sodium