Association of p60src with Triton X-100-resistant cellular structure correlates with morphological transformation.
More than 70% of wild-type Rous sarcoma virus p60v-src was found to be associated with a cellular structure resistant to nonionic detergent extraction that consists primarily of cytoskeletal proteins. On the other hand, nontransforming src proteins, including cellular p60c-src, nonmyristoylated forms, and those inactive in protein kinase, were found in the fraction solubilized by the detergent extraction. p60c-src was detergent-soluble even in transformed cells, suggesting that the association of p60v-src is not a result of cell transformation. Analyses with a variety of Rous sarcoma virus mutants showed a good correlation between the degree of association with the detergent-resistant structure and the extent of cell transformation caused by mutant src proteins, suggesting that this association may be significant for the process of cell transformation by Rous sarcoma virus.
D000818:Animals; D001358:Avian Sarcoma Viruses / Q000235:genetics*; D002471:Cell Transformation, Neoplastic; D002642:Chick Embryo; D003902:Detergents / Q000494:pharmacology*; D004351:Drug Resistance; D005347:Fibroblasts / Q000166:cytology / Q000201:enzymology; D017830:Octoxynol; D015688:Oncogene Protein pp60(v-src); D009857:Oncogenes; D011092:Polyethylene Glycols / Q000494:pharmacology*; D011494:Protein Kinases / Q000378:metabolism*; D012191:Retroviridae Proteins / Q000378:metabolism*; D013501:Surface-Active Agents / Q000494:pharmacology*
D003902:Detergents; D012191:Retroviridae Proteins; D013501:Surface-Active Agents; D011092:Polyethylene Glycols; D017830:Octoxynol; D011494:Protein Kinases; D015688:Oncogene Protein pp60(v-src)