Linear Percoll gradient centrifugation of rat anterior pituitary cells. A simple method for prolactin cell enrichment.
Prolactin (PRL) cells were purified from nulliparous normal female adult Wistar rat pituitary cell suspensions by linear Percoll density gradient centrifugation, a procedure yielding single cells. Lactotrophs were found in two different layers, the first containing 70% PRL cells in the density range 1.055 to 1.065 g/ml, the second with 28% PRL cells in the range 1.070 to 1.080 g/ml. Both cell fractions contained more than 90% viable cells with an intact ultrastructure. The physiological integrity of the 70% enriched PRL cells was assessed by their basal PRL secretion, their secretory response to TRH and dopamine, and their cAMP production in a basal situation and after incubation with dopamine.
D000818:Animals; D002499:Centrifugation, Density Gradient; D002500:Centrifugation, Isopycnic; D003584:Cytological Techniques; D005260:Female; D008854:Microscopy, Electron; D010903:Pituitary Gland, Anterior / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D011205:Povidone; D011388:Prolactin / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012822:Silicon Dioxide
C016039:Percoll; D012822:Silicon Dioxide; D011388:Prolactin; D011205:Povidone