v-myb does not prevent the expression of c-myb in avian erythroblasts.
The v-myb oncogene of avian myeloblastosis virus transforms myeloid cells exclusively, both in vivo and in vitro. The c-myb proto-oncogene from which v-myb arose is expressed at relatively high levels in immature hematopoietic cells of the lymphoid, erythroid, and myeloid lineages but not in myeloblasts transformed by v-myb. This finding suggested that the nuclear v-myb gene product p48v-myb might act directly to inhibit the normal expression of the c-myb gene. I have therefore used a selectable avian retroviral vector to express p48v-myb in avian erythroblasts which normally express high levels of the c-myb gene product p75c-myb. The results demonstrate that p48v-myb and p75c-myb can be coexpressed in the nuclei of cloned cells. Therefore, p48v-myb does not invariably prevent the expression of p75c-myb.
D000818:Animals; D001354:Avian Leukosis Virus / Q000235:genetics*; D009189:Avian Myeloblastosis Virus / Q000235:genetics*; D002460:Cell Line; D003001:Cloning, Molecular; D004900:Erythroblasts / Q000382:microbiology*; D005786:Gene Expression Regulation; D005814:Genes, Viral; D005822:Genetic Vectors; D020626:Oncogene Proteins v-myb; D009857:Oncogenes; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis / Q000235:genetics; D020598:Proto-Oncogene Proteins c-myb; D011519:Proto-Oncogenes; D012191:Retroviridae Proteins / Q000096:biosynthesis / Q000235:genetics; D014170:Transformation, Genetic
D020626:Oncogene Proteins v-myb; D011518:Proto-Oncogene Proteins; D020598:Proto-Oncogene Proteins c-myb; D012191:Retroviridae Proteins