Influence of hyperthermia, pH and culturing conditions on the electrical parameters of Chinese hamster V79 cells.
Conductivity measurements in the frequency range 10 kHz-100 MHz have been performed on Chinese hamster fibroblasts V79 exposed to hyperthermia in different experimental conditions. The membrane electrical conductivity is decreased when cells are heated for 1 h at 44 degrees C or 3 h at 43 degrees C at pH 7.25. This effect has not been observed on cell samples following heating for 1 h at 43 degrees C at pH 7.25. However, if the pH of the culture medium is adjusted to 6.50 or 6.0 before hyperthermic exposure, a substantial decrease of membrane conductivity is observed. In cells maintained in partially spent medium before heating, a 3 h treatment at 43 degrees C at pH 7.25 does not produce any effect on membrane conductivity. The results of all these experiments seem to indicate that some alteration of the energy-driven mechanisms involved in active transport across the plasma membranes may be responsible for the observed effects.
D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000502:physiology*; D006224:Cricetinae; D003412:Cricetulus; D003470:Culture Media; D004553:Electric Conductivity; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration
D003470:Culture Media