First report of an epidemic of diarrhoea in human neonates involving the new rotavirus and biological characteristics of the epidemic virus strain (KMB/R85).
An outbreak of diarrhoea in neonates occurred at the nurseries of the Department of Obstetrics of Zhao Tong Regional Hospital, Yunnan Province, from the middle of August to the end of November, 1985. Fifty-one percent of children were affected 2-8 days after birth. The clinical symptoms were mild; patients mainly had diarrhoea and did not vomit. Rotaviruses were detected in 66.7% by RNA PAGE and in 72.7% by EM. The virus strain designated as KMB/R85 had a typical morphology, which was indistinguishable from that of infantile rotaviruses by EM. The viral RNA genome was composed of 11 segments. The buoyant density in CsCl was 1.377 g/cm3. The KMB/R85 strain possessed a hemagglutinin for rhesus monkey erythrocytes. By ELISA, IEM, and HAI, it was found that KMB/R85 strain did not possess the common group antigen shared by group A rotaviruses and was antigenically similar to the Chinese adult diarrhoea rotavirus (serogroup B).
D002500:Centrifugation, Isopycnic; D003968:Diarrhea, Infantile / Q000382:microbiology*; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006387:Hemagglutination, Viral; D006801:Humans; D007231:Infant, Newborn; D008854:Microscopy, Electron; D012367:RNA, Viral / Q000235:genetics; D012401:Rotavirus / Q000235:genetics / Q000302:isolation & purification*
D012367:RNA, Viral