Uptake of low density lipoproteins by the hamster lung. Interactions with capillary endothelium.
The mechanism by which the circulating low density lipoproteins (LDL) contribute to the lung surfactant cholesterol was investigated by perfusing the hamster lung in situ with LDL either radiolabeled or coupled to gold, or both. Part of [125I]-LDL and [3H]-cholesterol LDL were taken up by a specific process which was time- and concentration-dependent and reached saturation within 20 to 30 min of perfusion. Competition experiments and removal of receptor-bound LDL by heparin suggested that about 50% of LDL uptake is receptor-independent. Experiments using double labeled LDL showed a preferential uptake of 3H-cholesterol versus 125I by the lung both in situ and in vivo. LDL-gold particles (LDL-Au), recirculated through the isolated lung, bound to the endothelial luminal plasma membrane and to features potentially involved in receptor-mediated endocytosis (coated pits, coated vesicles, lysosomelike structures) and in transcytosis (plasmalemmal vesicles). The results suggest that LDL uptake by the lung takes place by both receptor-mediated and receptor-independent mechanisms. Cholesterol may be in part transferred to the lung without the apoprotein moiety; the alveolar capillary endothelium appears to be the first monitor of this complex process.
D000818:Animals; D001667:Binding, Competitive; D002196:Capillaries / Q000378:metabolism / Q000648:ultrastructure; D008078:Cholesterol, LDL / Q000378:metabolism; D006224:Cricetinae; D004305:Dose-Response Relationship, Drug; D004727:Endothelium / Q000098:blood supply / Q000378:metabolism / Q000648:ultrastructure; D006048:Gold Colloid, Radioactive; D007457:Iodine Radioisotopes; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008168:Lung / Q000098:blood supply / Q000378:metabolism* / Q000648:ultrastructure; D008297:Male; D008647:Mesocricetus; D011973:Receptors, LDL / Q000378:metabolism; D013997:Time Factors; D014316:Tritium
D008078:Cholesterol, LDL; D006048:Gold Colloid, Radioactive; D007457:Iodine Radioisotopes; D008077:Lipoproteins, LDL; D011973:Receptors, LDL; D014316:Tritium