[3H]-nitrendipine binding in membranes obtained from hypoxic and reoxygenated heart.
We compared the binding properties of [3H]-nitrendipine in heart membranes from normal guinea-pig heart and from hypoxic or hypoxic and reoxygenated heart. The [3H]-nitrendipine binds a single class of high capacity (Bmax 667.2 +/- 105.2) with high affinity (KD 0.14 +/- 0.02) binding sites. By contrast, in membranes of hypoxic and reoxygenated heart the Bmax decreases significantly while it remains unaffected during hypoxia. Xanthinoxidase activity is increased in hypoxic-reoxygenated hearts.
D000332:Aerobiosis; D000693:Anaerobiosis; D000818:Animals; D015220:Calcium Channels; D002462:Cell Membrane / Q000378:metabolism; D006168:Guinea Pigs; D006352:Heart Ventricles / Q000378:metabolism; D000860:Hypoxia / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D008861:Microsomes / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism*; D009568:Nitrendipine / Q000378:metabolism*; D011978:Receptors, Nicotinic / Q000378:metabolism*; D014969:Xanthine Oxidase / Q000378:metabolism
D015220:Calcium Channels; D011978:Receptors, Nicotinic; D009568:Nitrendipine; D014969:Xanthine Oxidase