Identification of p40x-responsive regulatory sequences within the human T-cell leukemia virus type I long terminal repeat.
Distinct transcriptional regulatory sequences located within the upstream sequences required for p40x trans-activation of the human T-cell leukemia virus type I (HTLV-I) long terminal repeat (LTR) were chemically synthesized and cloned upstream of the basal HTLV-I LTR promoter. Plasmids containing a single 21-base-pair (bp) repeat were weakly inducible by p40x. The level of trans-activation by p40x was increased when two (30-fold) or three (40-fold) 21-bp repeats were present in the upstream control region. In the mutant containing two 21-bp repeats, the upstream 21-bp repeat could be positioned in either the sense (30-fold) or the antisense (16-fold) orientation. Plasmids containing a 51-bp repeat element, which included a single 21-bp repeat, were induced to levels similar to that obtained with the 21-bp repeat sequence alone. Template DNAs containing a single copy of the HTLV-I sequences between -117 and -160 were stimulated approximately 10-fold by p40x when one copy of the 21-bp element was located downstream.
D001483:Base Sequence; D004279:DNA, Viral / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000502:physiology*; D017977:Deltaretrovirus / Q000235:genetics*; D005786:Gene Expression Regulation; D005809:Genes, Regulator; D005813:Genes, Synthetic; D005814:Genes, Viral; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000235:genetics; D011401:Promoter Regions, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D012191:Retroviridae Proteins / Q000502:physiology*; D015534:Trans-Activators; D014157:Transcription Factors / Q000502:physiology*; D014158:Transcription, Genetic
D004279:DNA, Viral; D004268:DNA-Binding Proteins; D009838:Oligodeoxyribonucleotides; D012191:Retroviridae Proteins; D015534:Trans-Activators; D014157:Transcription Factors