Antiglomerular basement membrane nephritis in the mouse. Study on the role of complement in the heterologous phase.
The role of complement was examined in a model of antiglomerular basement membrane nephritis in the mouse, induced by intravenous injection of goat anti-mouse glomerular basement membrane serum, and characterized by early glomerular lesions and a dose-dependent albuminuria. We compared the reaction after injection of the antiserum in complement-normal B10.D2 new mice with that in congenic, congenitally C5-deficient B10.D2 old mice, and in both strains after C3 depletion by treatment with cobra venom factor. The dose-dependent albuminuria was not affected by the absence of complement activation. Also, deficiency of C3 and/or C5 had no inhibitory effect on the histologic glomerular lesions: it did not reduce the influx of polymorphonuclear granulocytes in the glomerular capillary vessels, nor prevented the eventual intravascular coagulation. We conclude that complement-independent mechanisms are involved in the development of the heterologous phase of antiglomerular basement membrane nephritis in the mouse.
D000419:Albuminuria / Q000652:urine; D000818:Animals; D001485:Basement Membrane / Q000276:immunology / Q000648:ultrastructure; D003176:Complement C3 / Q000032:analysis / Q000172:deficiency / Q000276:immunology; D003182:Complement C5 / Q000172:deficiency / Q000276:immunology; D003165:Complement System Proteins / Q000032:analysis / Q000172:deficiency / Q000276:immunology*; D004195:Disease Models, Animal; D005455:Fluorescent Antibody Technique; D007678:Kidney Glomerulus / Q000276:immunology* / Q000648:ultrastructure; D007700:Kinetics; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008854:Microscopy, Electron; D009393:Nephritis / Q000209:etiology* / Q000276:immunology / Q000473:pathology
D003176:Complement C3; D003182:Complement C5; D003165:Complement System Proteins