Activation of collagenase production in a rat macrophage-like cell line.
Collagenase activity from a rat macrophage line, AK-5, has been studied. The enzyme is present in these cells and its release is stimulated by Con A, LPS and a lymphokine preparation from rat spleen cells. Collagenase activity from these cells is inhibited by EDTA, cysteine or dithiothreitol, thereby suggesting it to be similar to other mammalian collagenases.
D000276:Adjuvants, Immunologic; D000818:Animals; D002460:Cell Line; D003094:Collagen; D004789:Enzyme Activation; D007700:Kinetics; D008264:Macrophages / Q000201:enzymology*; D003012:Microbial Collagenase / Q000378:metabolism*; D051381:Rats
D000276:Adjuvants, Immunologic; D003094:Collagen; D003012:Microbial Collagenase