Quantitative analytical aspects of reversed-phase liquid chromatography with slurry-packed capillary columns.
Conditions for reproducible packing of fused-silica liquid chromatography capillary columns were demonstrated. The plate height vs. velocity curves were determined for successively prepared columns. In addition, the values of separation impedance were calculated. Several liquid chromatography systems were evaluated in conjunction with the slurry-packed capillaries for the retention and peak area reproducibility.
D002853:Chromatography, Liquid / Q000295:instrumentation*; D004058:Diffusion; D006801:Humans; D006914:Hydroxysteroids / Q000652:urine; D010316:Particle Size; D010539:Permeability; D012822:Silicon Dioxide; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet
D006914:Hydroxysteroids; D012997:Solvents; D012822:Silicon Dioxide