Tn5 insertion mutants of Pseudomonas aeruginosa deficient in surface expression of ferripyochelin-binding protein.
Transposon (Tn5) insertion mutants were isolated in Pseudomonas aeruginosa PAO. These mutants were screened for expression of the ferripyochelin-binding protein with monoclonal antibody in a whole-cell immunoblot assay. Fourteen mutants were identified which did not express ferripyochelin-binding protein on the cell surface. These mutants did not take up 59Fe-labeled pyochelin and grew slowly in the presence of iron chelators.
D000911:Antibodies, Monoclonal; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D001426:Bacterial Proteins; D002352:Carrier Proteins / Q000235:genetics* / Q000276:immunology; D004251:DNA Transposable Elements; D007501:Iron / Q000378:metabolism; D008565:Membrane Proteins; D008970:Molecular Weight; D009154:Mutation; D010636:Phenols / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000235:genetics*; D013844:Thiazoles
D000911:Antibodies, Monoclonal; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D002352:Carrier Proteins; D004251:DNA Transposable Elements; D008565:Membrane Proteins; D010636:Phenols; D013844:Thiazoles; C043809:ferripyochelin-binding protein, Pseudomonas aeruginosa; C025316:pyochelin; D007501:Iron