The 65,000-Mr DNA-binding and virion trans-inducing proteins of herpes simplex virus type 1.
The possible identity of the herpes simplex virus type 1 (HSV-1) 65K (65,000-Mr) virion protein which stimulates transcription from immediate-early genes with the HSV-1 65K DNA-binding protein was investigated. The two proteins were found to be distinct by the three separate criteria of immunological reactivity, tryptic peptide fingerprinting, and mobility in two-dimensional gels. Using HSV-1/HSV-2 intertypic recombinants and a serotype-specific antiserum, we located the gene encoding the 65K DNA-binding protein between coordinates 0.574 and 0.682 on the HSV-1 genome. The protein is posttranslationally modified by phosphorylation. In crude extracts of HSV-1-infected cells the 65K trans-inducing protein did not detectably bind to double-stranded calf thymus DNA under the conditions of our assay.
D000956:Antigens, Viral / Q000032:analysis; D002874:Chromosome Mapping; D004268:DNA-Binding Proteins / Q000378:metabolism*; D005814:Genes, Viral; D007158:Immunologic Techniques; D008970:Molecular Weight; D011499:Protein Processing, Post-Translational; D018139:Simplexvirus / Q000235:genetics* / Q000276:immunology; D014157:Transcription Factors / Q000378:metabolism*; D014764:Viral Proteins / Q000145:classification / Q000302:isolation & purification / Q000502:physiology*; D014771:Virion / Q000502:physiology
D000956:Antigens, Viral; D004268:DNA-Binding Proteins; D014157:Transcription Factors; D014764:Viral Proteins