[Effect of ionizing radiation on O2 generation by cytochrome P-450].
A study was made of generation of superoxide anion-radical (O2-) by cytochrome P-450 in a microsomal membrane of rat liver. Using spectrophotometry (by oxidation of adrenaline to adrenochrome) and ESR (with a spin-trap, tiron) the authors showed the ability of O2- generation by P-450 through decomposition of organic peroxides. During the first 24 h following irradiation of rats with doses of 7 and 10 Gy, the generation of O2- by cytochrome P-450 of rat liver microsomes was increased. Mechanisms of the postirradiation modification of O2- generation rate are discussed.
D014013:1,2-Dihydroxybenzene-3,5-Disulfonic Acid Disodium Salt / Q000378:metabolism; D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism / Q000528:radiation effects*; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism / Q000528:radiation effects; D051381:Rats; D011919:Rats, Inbred Strains; D013113:Spin Labels; D013481:Superoxides / Q000378:metabolism / Q000528:radiation effects*
D013113:Spin Labels; D013481:Superoxides; D014013:1,2-Dihydroxybenzene-3,5-Disulfonic Acid Disodium Salt; D003577:Cytochrome P-450 Enzyme System
Vliianie ioniziruiushcheĭ radiatsii na generatsiiu O2 tsitokhromom P-450.