title: Mumps virus infection of dissociated rodent spinal ganglia in vitro. Expression and disappearance of viral structural proteins from neurons.
abstract: Cultured spinal ganglia and cord from mice and hamsters were infected with mumps virus or Sendai virus. Expression of five structural proteins, the haemagglutinin-neuraminidase, fusion, nucleocapsid (NP), phospho (P) and matrix proteins was examined with monoclonal antibodies to each protein. In Sendai virus-infected mouse neurons all five viral proteins were detected. In hamster neurons infected with mumps virus all viral proteins were also expressed, but in mouse neurons only the NP and P proteins were seen. This suggests a species-specific cellular restriction of viral protein synthesis in mumps virus-infected mouse neurons. There was no, or only a slight, reduction in the number of neurons between days 4 and 20 after infection of mouse cultures with mumps virus, but the proportion of infected neurons diminished from 68% to 15% during this time.
mesh_terms: D000818:Animals; D002213:Capsid / Q000096:biosynthesis; D006224:Cricetinae; D046508:Culture Techniques; D005727:Ganglia, Spinal / Q000382:microbiology*; D015707:HN Protein; D051379:Mice; D009109:Mumps virus / Q000378:metabolism*; D009474:Neurons / Q000382:microbiology*; D010222:Parainfluenza Virus 1, Human / Q000378:metabolism; D010750:Phosphoproteins / Q000096:biosynthesis; D013045:Species Specificity; D014758:Viral Core Proteins / Q000096:biosynthesis; D014759:Viral Envelope Proteins / Q000096:biosynthesis; D014760:Viral Fusion Proteins / Q000096:biosynthesis; D014763:Viral Matrix Proteins; D014764:Viral Proteins / Q000096:biosynthesis*; D015678:Viral Structural Proteins
chemical_list: D015707:HN Protein; D010750:Phosphoproteins; D014758:Viral Core Proteins; D014759:Viral Envelope Proteins; D014760:Viral Fusion Proteins; D014763:Viral Matrix Proteins; D014764:Viral Proteins; D015678:Viral Structural Proteins