Detection of salmonellas in confectionery products by conductance.
A modified lysine decarboxylase broth has been developed which could be used with a Bactometer M123 to differentiate salmonellas from other bacteria by the characteristics of the conductance detection curve. The medium was used in combination with a selenite cystine trimethylamine oxide dulcitol medium to screen 50 strains of salmonellas and 42 strains of other organisms to establish detection curve magnitude and rate values which could be used to identify curves specific to salmonellas. The combination of media detected all salmonellas tested except Salmonella pullorum. The two media were used to screen 100 inoculated product samples with the Bactometer instrument, in parallel with traditional plating procedures, and using various combinations of pre-enrichment and selective enrichment incubation periods. After 24 h pre-enrichment, the Bactometer system detected more positive samples than the conventional plating procedures after pre-enrichment and selective enrichment. It is considered that these media used in parallel in the Bactometer after conventional pre-enrichment could provide a 48 h screening procedure for salmonellas with a sensitivity comparable to present plating procedures.
D001431:Bacteriological Techniques; D002262:Carboxy-Lyases / Q000378:metabolism; D003470:Culture Media; D004553:Electric Conductivity; D005296:Ferrous Compounds; D005516:Food Microbiology; D000644:Quaternary Ammonium Compounds; D012475:Salmonella / Q000302:isolation & purification* / Q000378:metabolism; D013885:Thiosulfates
D003470:Culture Media; D005296:Ferrous Compounds; D000644:Quaternary Ammonium Compounds; D013885:Thiosulfates; C038178:ammonium ferrous sulfate; D002262:Carboxy-Lyases; C021520:lysine decarboxylase; C017717:sodium thiosulfate