title: Optical approaches to the study of foraminiferan motility.
abstract: Microtubules are the major cytoskeletal component of foraminiferan reticulopodia. Video-enhanced differential interference contrast light microscopy has demonstrated that the microtubules serve as the intracellular tracks along which rapid bidirectional organelle transport and cell surface motility occurs. Microtubules appear to move, both axially and laterally within the pseudopodial cytoplasm, and these microtubule translocations appear to drive the various reticulopodial movements. F-actin is localized to discrete filament plaques form at sites of pseudopod-substrate adhesion. Correlative immunofluorescence and electron microscopy reveals a structural interaction between microtubules and the actin-containing filament plaques. Our recent data on reticulopodial motility are discussed in an historical context, and a model for foram motility, based on motile microtubules, is presented.
mesh_terms: D000199:Actins / Q000032:analysis; D000818:Animals; D002465:Cell Movement; D056890:Eukaryota; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D008858:Microscopy, Phase-Contrast; D008870:Microtubules / Q000648:ultrastructure; D015388:Organelles / Q000648:ultrastructure; D011554:Pseudopodia / Q000648:ultrastructure; D014741:Video Recording
chemical_list: D000199:Actins