title: Characterization of rat schwannoma-Schwann cell hybrids.
abstract: Sciatic nerve Schwann cells from strain LEC rats, homozygous for the c form of 6-phosphogluconate dehydrogenase (6-PGD), and RN22 rat Schwannoma cells, a subclone of RN2 deficient in hypoxanthine phosphoribosyltransferase and expressing the s form of 6-PGD, were fused to produce 'RNS' hybrid clones which proliferate rapidly in a medium containing hypoxanthine, aminopterin and thymidine (HAT) and express c, s and c/s heterodimeric forms of 6-PGD. RNS cells, like both parents, maintain a high baseline activity of 2', 3'-cyclic nucleotide 3'-phosphohydrolase and, as in RN22, activity of this enzyme is further inducible by 1 mM N6, O2'-dibutyryl 3', 5'-cyclic AMP. The RNS clones resemble normal Schwann cells in the capacity to bind radioiodinated axolemmal fragments to their plasma membranes.
mesh_terms: D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases / Q000378:metabolism; D000818:Animals; D001369:Axons / Q000378:metabolism; D002448:Cell Adhesion; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D002875:Chromosomes / Q000032:analysis; D006822:Hybrid Cells / Q000201:enzymology / Q000378:metabolism*; D007621:Karyotyping; D009442:Neurilemmoma / Q000201:enzymology / Q000378:metabolism*; D010734:Phosphogluconate Dehydrogenase / Q000378:metabolism; D051381:Rats; D012583:Schwann Cells / Q000201:enzymology / Q000378:metabolism*; D012584:Sciatic Nerve / Q000166:cytology
chemical_list: D010734:Phosphogluconate Dehydrogenase; D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases