title: The human preproapolipoprotein C-II gene. Complete nucleic acid sequence and genomic organization.
abstract: The complete nucleic acid sequence of human preproapolipoprotein (apo) C-II has been determined from 2 apoC-II clones isolated from 2 different human genomic DNA libraries. The cloned fragments were approx. 14 and 18 kb long, and sequence analysis established that the apoC-II gene consists of 3338 nucleotides containing 3 intervening sequences of 2391, 167, and 298 bases. The first intron is located within the 5'-untranslated region of apoC-II and contains 4 Alu type sequences. The second intron interrupts the codon specifying amino acid - 11 of the apoC-II signal peptide. The last intron, which contains a 38 bp sequence which is repeated 6 times, interrupts the codon specifying for amino acid +44 of the mature apolipoprotein.
mesh_terms: D053304:Apolipoprotein C-II; D001056:Apolipoproteins C / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes / Q000378:metabolism; D015245:Deoxyribonuclease BamHI; D015246:Deoxyribonuclease EcoRI; D006801:Humans; D007438:Introns; D009690:Nucleic Acid Conformation; D011498:Protein Precursors / Q000235:genetics*
chemical_list: D053304:Apolipoprotein C-II; D001056:Apolipoproteins C; D011498:Protein Precursors; C048859:preproapolipoprotein C2; D004262:DNA Restriction Enzymes; D015245:Deoxyribonuclease BamHI; D015246:Deoxyribonuclease EcoRI