title: Sequential morphologic and biochemical studies of naturally occurring wheat-sensitive enteropathy in Irish setter dogs.
abstract: This study has investigated the potential role of wheat in the pathogenesis of a naturally occurring enteropathy in Irish setter dogs. At eight months on a cereal-containing diet, jejunal biopsies from affected animals exhibited partial villus atrophy, increased intraepithelial lymphocytes, and distinct biochemical abnormalities in the brush border. Activities of alkaline phosphatase and leucyl-2-naphthylamidase were almost undetectable while disaccharidases were unaltered. Activity of 5'-nucleotidase (basolateral membrane) was low, and reduced malate dehydrogenase reflected a loss of mitochondrial activity, but other organelles were unaffected. Recovery was achieved on a wheat-free diet. Relapse on subsequent wheat challenge was characterized by partial villus atrophy and a selective effect on the brush border: modal density was decreased and there was a severe loss of brush-border alkaline phosphatase activity. These findings document a wheat-sensitive enteropathy in Irish setter dogs and suggest that brush-border alkaline phosphatase is specifically susceptible to damage by wheat.
mesh_terms: D015720:5'-Nucleotidase; D000367:Age Factors; D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D002446:Celiac Disease / Q000201:enzymology / Q000473:pathology / Q000662:veterinary*; D004195:Disease Models, Animal; D004283:Dog Diseases / Q000201:enzymology / Q000473:pathology*; D004285:Dogs; D005260:Female; D006651:Histocytochemistry; D007413:Intestinal Mucosa / Q000201:enzymology / Q000473:pathology; D007583:Jejunum / Q000201:enzymology / Q000473:pathology*; D007931:Leucyl Aminopeptidase / Q000032:analysis; D008291:Malate Dehydrogenase / Q000032:analysis; D008297:Male; D009708:Nucleotidases / Q000032:analysis; D014908:Triticum / Q000009:adverse effects
chemical_list: D008291:Malate Dehydrogenase; D009708:Nucleotidases; D000469:Alkaline Phosphatase; D015720:5'-Nucleotidase; D007931:Leucyl Aminopeptidase