title: Bactericidal efficacy of metronidazole against bacteria of human carious dentin in vitro.
abstract: The bactericidal efficacy of metronidazole against bacteria in carious dentin was clarified by measuring (1) the difference between bacterial recovery from suspensions of carious dentin on metronidazole-containing BHI-Blood agar plates (10 micrograms/ml) and control plates and (2) the difference between bacterial recovery from carious lesions of freshly extracted teeth, covered by alpha-tricalcium phosphate (TCP) cement containing metronidazole (5%) for 1-3 days and that covered by TCP only. More than 10(3) bacteria per milligram sample were recovered from carious dentin. More than 99% of the bacteria were, however, not recovered when samples were inoculated on metronidazole-containing BHI-Blood agar plates or when the lesions were covered by TCP cement containing metronidazole, indicating that metronidazole effectively disinfected the carious dentin.
mesh_terms: D001419:Bacteria / Q000187:drug effects*; D001431:Bacteriological Techniques; D002130:Calcium Phosphates; D003731:Dental Caries / Q000382:microbiology*; D003738:Dental Cements; D003804:Dentin / Q000382:microbiology*; D006801:Humans; D008795:Metronidazole / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D002130:Calcium Phosphates; D003738:Dental Cements; C485828:alpha-tricalcium phosphate; C485830:tetracalcium phosphate; D008795:Metronidazole; C485838:calcium phosphate, monobasic, anhydrous; C020243:calcium phosphate; C485829:calcium phosphate, dibasic, anhydrous