The endosomal concentration of a mannose 6-phosphate receptor is unchanged in the absence of ligand synthesis.
The cation-independent mannose-6-phosphate (Man-6-P) receptor is involved in the targeting of newly synthesized lysosomal hydrolases. To investigate the intracellular distribution of this receptor, a conjugate of lactoperoxidase coupled to asialoorosomucoid was used to catalyze its iodination within the endosomes of human hepatoma (HepG2) cells. The 215-kD, cation-independent Man-6-P receptor was iodinated by this procedure as shown by pentamannosyl-6-phosphate-Sepharose affinity chromatography and by immunoprecipitation of labeled cell extracts. The amount of this receptor detected in endosomes was found to be unchanged after inhibition of protein synthesis with cycloheximide. If the Man-6-P receptor accumulates in the Golgi apparatus in the absence of lysosomal hydrolase synthesis, it should have been correspondingly depleted from endosomes after a period of cycloheximide treatment, because these pools of receptor are in rapid equilibrium. Therefore, these data suggest that newly synthesized ligands are not required for the transport of the cation-independent Man-6-P receptor from the Golgi apparatus to endosomes.
D001212:Asialoglycoproteins; D006528:Carcinoma, Hepatocellular / Q000473:pathology; D002352:Carrier Proteins / Q000032:analysis*; D002460:Cell Line; D003513:Cycloheximide / Q000494:pharmacology; D004705:Endocytosis; D006056:Golgi Apparatus / Q000378:metabolism; D006801:Humans; D006867:Hydrolases / Q000096:biosynthesis; D007553:Isotope Labeling / Q000379:methods; D007784:Lactoperoxidase; D008113:Liver Neoplasms / Q000473:pathology; D008247:Lysosomes / Q000032:analysis* / Q000201:enzymology; D009961:Orosomucoid / Q000031:analogs & derivatives / Q000378:metabolism; D014176:Protein Biosynthesis / Q000187:drug effects; D011499:Protein Processing, Post-Translational; D017527:Receptor, IGF Type 2
D001212:Asialoglycoproteins; D002352:Carrier Proteins; D009961:Orosomucoid; D017527:Receptor, IGF Type 2; C017030:asialoorosomucoid; D003513:Cycloheximide; D007784:Lactoperoxidase; D006867:Hydrolases