title: Rapid purification of tonin, esterase B, antigen psi and kallikrein from rat submandibular gland by fast protein liquid chromatography.
abstract: Tonin, esterase B, antigen psi and kallikrein from the rat submandibular gland were purified by fast protein liquid chromatography with Mono P or Mono Q columns. The purity of the separated proteins was evaluated by sodium dodecyl sulphate polyacrylamide gel electrophoresis and by isoelectrofocusing in flat-bed polyacrylamide gel. Tonin and esterase B were purified by DE-52 cellulose anion-exchange chromatography and chromatofocusing on Mono P in two and three steps, respectively. Antigen psi and kallikrein were purified by a two-step procedure using DE-52 cellulose and Mono Q anion-exchange chromatography. The high resolution power of Mono Q revealed the different isoenzymes of kallikrein.
mesh_terms: D000818:Animals; D000941:Antigens / Q000302:isolation & purification*; D002848:Chromatography, DEAE-Cellulose; D002852:Chromatography, Ion Exchange; D010450:Endopeptidases / Q000302:isolation & purification*; D007122:Immunoelectrophoresis; D007525:Isoelectric Focusing; D007610:Kallikreins / Q000302:isolation & purification*; D007703:Peptidyl-Dipeptidase A / Q000302:isolation & purification*; D051381:Rats; D011919:Rats, Inbred Strains; D012697:Serine Endopeptidases; D013363:Submandibular Gland / Q000032:analysis*
chemical_list: D000941:Antigens; D010450:Endopeptidases; D007703:Peptidyl-Dipeptidase A; D007610:Kallikreins; D012697:Serine Endopeptidases; C049262:esterase B