A novel mechanism for utilization of extracellular AMP in Vibrio parahaemolyticus.
Vibrio parahaemolyticus could grow with AMP, ADP or ATP as the sole source of carbon. In the presence of Cl-, a membrane-bound Cl(-)-dependent 5'-nucleotidase seemed to hydrolyze the nucleotides extracellularly, and then the cells took up the resulting adenosine. In the absence of Cl-, although no significant dephosphorylation of the nucleotides occurred, the cells could still grow with AMP, but not with ADP or ATP. Moreover, in the presence of Cl-, Zn2+ inhibited the 5'-nucleotidase, and inhibited growth of the cells with ADP or ATP, but not with AMP, as the carbon source. V. parahaemolyticus was unable to grow with adenine or ribose 5-phosphate. These results suggested that the cells might have an AMP transport system. In fact, Na+ uptake was observed on addition of AMP to a cell suspension in the absence of Cl-, indicating Na+-AMP cotransport.
D015720:5'-Nucleotidase; D000249:Adenosine Monophosphate / Q000378:metabolism*; D001693:Biological Transport, Active / Q000187:drug effects; D002462:Cell Membrane / Q000378:metabolism; D002712:Chlorides / Q000494:pharmacology; D005110:Extracellular Space / Q000378:metabolism; D009708:Nucleotidases / Q000378:metabolism; D012964:Sodium / Q000378:metabolism; D014736:Vibrio parahaemolyticus / Q000378:metabolism*; D015032:Zinc / Q000494:pharmacology
D002712:Chlorides; D000249:Adenosine Monophosphate; D012964:Sodium; D009708:Nucleotidases; D015720:5'-Nucleotidase; D015032:Zinc