Some properties of subunits of DNA gyrase from Pseudomonas aeruginosa PAO1 and its nalidixic acid-resistant mutant.
Subunits A and B of DNA gyrase were purified from Pseudomonas aeruginosa PAO1 and its mutant, which was resistant to nalidixic acid. Inhibition tests of DNA gyrases reconstituted with a combination of subunits from the two strains showed that an alteration of subunit A but not subunit B caused bacteria to resist fluoroquinolones.
D004250:DNA Topoisomerases, Type II / Q000235:genetics*; D004278:DNA, Superhelical; D004352:Drug Resistance, Microbial; D009154:Mutation; D009268:Nalidixic Acid / Q000494:pharmacology; D009675:Novobiocin / Q000494:pharmacology; D011550:Pseudomonas aeruginosa / Q000201:enzymology / Q000235:genetics*; D059005:Topoisomerase II Inhibitors
D004278:DNA, Superhelical; D059005:Topoisomerase II Inhibitors; D009675:Novobiocin; D009268:Nalidixic Acid; D004250:DNA Topoisomerases, Type II