title: A bacterial protein requirement for the bacteriophage lambda terminase reaction.
abstract: The bacteriophage lambda terminase enzyme cleaves the cohesive-end sites of lambda DNA to yield the protruding 5'-termini of the mature molecule. In vitro, this endonucleolytic event requires a protein factor which has been isolated and purified from extracts of uninfected E. coli. The terminase host factor (THF) is a heat stable basic protein of M.W. approximately 22,000. The integration host factor (IHF) protein of E. coli can efficiently substitute for THF in the terminase reaction; however, THF can be demonstrated to be physically present in, and isolated with full biological activity from extracts of cells defective or deficient in IHF.
mesh_terms: D001426:Bacterial Proteins / Q000302:isolation & purification / Q000378:metabolism*; D010582:Bacteriophage lambda / Q000201:enzymology* / Q000235:genetics; D003851:Deoxyribonucleases / Q000378:metabolism*; D004706:Endodeoxyribonucleases; D004720:Endonucleases / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D034982:Integration Host Factors; D008970:Molecular Weight; D010957:Plasmids
chemical_list: D001426:Bacterial Proteins; D034982:Integration Host Factors; D003851:Deoxyribonucleases; D004706:Endodeoxyribonucleases; D004720:Endonucleases; C030894:terminase