Evidence for the existence of an endogenous inhibitor of Na, K-ATPase in plasma from cats and rats.
Plasma samples from cats and rats were filtered through molecular filters to obtain a concentrate of the plasma molecules with a mass between 500 and 10,000. This concentrate was placed on a gel filtration column and eluted with a Tris buffer. Fractions corresponding to a molecular mass of 1500-2000 exhibited a marked Na,K-ATPase inhibitory 'activity', which was not influenced by heating to 95 degrees C for 10 min or by enzymatic degradation by pronase or trypsin.
D000818:Animals; D002415:Cats; D002850:Chromatography, Gel; D006358:Hot Temperature; D008970:Molecular Weight; D011402:Pronase / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors / Q000097:blood*; D014357:Trypsin / Q000494:pharmacology; D014462:Ultrafiltration
D014357:Trypsin; D011402:Pronase; D000254:Sodium-Potassium-Exchanging ATPase