title: The structure and cloning of orf virus DNA.
abstract: A map of cleavage sites for the restriction endonucleases EcoRI, HindIII, BamHI, HpaI, and KpnI for a New Zealand strain of orf virus (NZ2) DNA has been deduced. Also, the entire genome, apart from approximately 0.1 kbp at each end, has been cloned into various vectors. The genome is 139 kbp in length and, in common with other poxviruses, has inverted terminal repetitions and crosslinked ends.
mesh_terms: D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000235:genetics*; D005818:Genetic Engineering / Q000379:methods; D009693:Nucleic Acid Hybridization; D011212:Poxviridae / Q000235:genetics*
chemical_list: D004279:DNA, Viral; D004262:DNA Restriction Enzymes