title: Regulation of bacteriophage f1 DNA replication. I. New functions for genes II and X.
abstract: Gene II protein is required for all phases of filamentous phage DNA synthesis other than the conversion of the infecting single strand to the parental double-stranded molecule. It introduces a specific nick into the double-stranded replicative form DNA, is required for the initiation of (+) strand synthesis and is responsible for termination and ring closure of the (+) strand product. Here we show that the gene II protein also promotes minus strand synthesis later in infection. Over-expression of gene II protein can induce the conversion of all nascent single-stranded phage DNA to the double-stranded form, even in the presence of the single-stranded DNA-binding gene V protein that would normally sequester the newly synthesized single strands. We also present evidence that the gene X protein (separately translated from an initiator codon within gene II, and identical to the C-terminal one-third of the gene II protein) is a powerful inhibitor of phage-specific DNA synthesis in vivo.
mesh_terms: D001483:Base Sequence; D003090:Coliphages / Q000235:genetics; D004261:DNA Replication; D004279:DNA, Viral / Q000096:biosynthesis*; D004926:Escherichia coli; D005814:Genes, Viral; D007544:Isopropyl Thiogalactoside; D008969:Molecular Sequence Data; D010957:Plasmids; D014176:Protein Biosynthesis; D014764:Viral Proteins / Q000235:genetics*; D014779:Virus Replication
chemical_list: D004279:DNA, Viral; D014764:Viral Proteins; D007544:Isopropyl Thiogalactoside