title: Cryptic disposition of antigenic parasite proteins in plasma membranes of erythrocytes infected with Plasmodium chabaudi.
abstract: Plasma membranes of Plasmodium chabaudi-infected erythrocytes contain seven major neoproteins with apparent molecular masses of 154, 145, 90, 72, 67, 52, and 33 kDa, respectively. These neoproteins, with the exception of the two larger ones, can be metabolically labelled with [14C]isoleucine. The seven neoproteins are antigenic as revealed by Western blotting using hyperimmune sera obtained from two different mouse strains. None of the parasite proteins is accessible from the outside in intact P. chabaudi-infected erythrocytes as determined by lactoperoxidase-mediated radioiodination, indirect immune fluorescence microscopy, or post-embedding immunoelectron microscopy. These methods, however, identify parasite proteins in host cell plasma membranes when the latter are artificially changed either during isolation or by methanol fixation. We conclude therefore that parasitic proteins are cryptically arranged in intact host cell plasma membranes of P. chaubaudi-infected erythrocytes.
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000096:biosynthesis* / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism / Q000469:parasitology; D004910:Erythrocyte Membrane / Q000378:metabolism / Q000469:parasitology; D004912:Erythrocytes / Q000469:parasitology* / Q000648:ultrastructure; D007158:Immunologic Techniques; D007457:Iodine Radioisotopes; D007532:Isoleucine; D007553:Isotope Labeling; D008288:Malaria / Q000097:blood / Q000469:parasitology*; D051379:Mice; D008856:Microscopy, Fluorescence
chemical_list: D000953:Antigens, Protozoan; D007457:Iodine Radioisotopes; D007532:Isoleucine