title: Activation of hydrazine derivatives to free radicals in the perfused rat liver: a spin-trapping study.
abstract: Spin-trapping techniques and electron spin resonance spectroscopy have been used to study bioactivations of hydrazine and its derivatives by isolated perfused rat livers. Using phenylbutylnitrone (PBN) as the stable spin trap, it was found that the liver perfusion of hydrazine, acetylhydrazine and isoniazid resulted in the formation of the same carbon-centered radical which was shown to be the acetyl radical. The identity of the acetyl radical was confirmed after organic extraction of the liver perfusates, by comparing its coupling constants with those of the in vitro metal ion- or horseradish peroxidase-catalyzed oxidation products of the hydrazines in the same solvents. The liver perfusion of iproniazid formed the isopropyl radical which was previously observed to result from peroxidase-catalyzed oxidation.
mesh_terms: D000818:Animals; D001711:Biotransformation; D004578:Electron Spin Resonance Spectroscopy; D005609:Free Radicals; D006834:Hydrazines / Q000378:metabolism*; D007490:Iproniazid / Q000378:metabolism; D007538:Isoniazid / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D051381:Rats
chemical_list: D005609:Free Radicals; D006834:Hydrazines; C029424:hydrazine; D007490:Iproniazid; C014949:acetylhydrazine; D007538:Isoniazid