A new V gene expressed in lambda-2 light chains of the mouse.
We have partially sequenced the light chain variable regions expressed in three IgM-producing hybridomas generated from newborn mice or from manipulated animals suppressed for IgM production. In these lines a new V gene (V-lambda-X), exhibiting less than 60% homology to any known lambda or kappa V gene, is rearranged to J-lambda-2. The light chains produced by these cells contain the lambda-2 constant domain, but are not recognized by goat antisera raised against conventional mouse lambda light chains.
D000818:Animals; D001483:Base Sequence; D004262:DNA Restriction Enzymes; D005796:Genes; D006825:Hybridomas / Q000502:physiology; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D007146:Immunoglobulin lambda-Chains / Q000235:genetics*; D051379:Mice / Q000235:genetics*; D011995:Recombination, Genetic
D007135:Immunoglobulin Variable Region; D007146:Immunoglobulin lambda-Chains; D004262:DNA Restriction Enzymes