A transposable promoter and transposable promoter probes derived from Tn1721.
We describe two types of new Tn1721-derivatives capable of random insertion and of generating transcriptional fusions at the site of insertion: transposable promoters (Tn1735) carrying a strong, inducible ptac promoter that turns on adjacent (cryptic) genes; and transposable promoter probes (Tn1736, Tn1737) carrying promoterless genes coding for chloramphenicol acetyl transferase or beta-galactosidase, and used to accurately determine the expression of external promoters. These elements are available with four different selectable resistance markers and on conjugative, temperature-sensitive and multicopy plasmid vehicles. Experiments are described that demonstrate the advantage of random insertions for expressing various genes and for studying gene regulation.
D000123:Acetyltransferases / Q000235:genetics; D015500:Chloramphenicol O-Acetyltransferase; D004251:DNA Transposable Elements; D005818:Genetic Engineering / Q000379:methods; D009154:Mutation; D010957:Plasmids; D011401:Promoter Regions, Genetic; D001616:beta-Galactosidase / Q000235:genetics
D004251:DNA Transposable Elements; D000123:Acetyltransferases; D015500:Chloramphenicol O-Acetyltransferase; D001616:beta-Galactosidase