[Mechanism of growth limitations in a recombinant strain of Escherichia coli].
A recombinant Escherichia coli strain with the cloned gene of restriction endonuclease EcoR-V was studied. The diauxic growth of this strain under batch conditions, the composition of excreted products and the hyperbolic shape of the chemostat growth curve has made it possible to formulate the following hypothesis. At a high flow rate, one of the first reactions in the tricarboxylic acid cycle or a reaction directly preceding the cycle is a limiting step in the metabolism of E. coli cells. The saturation of the limiting reaction with a substrate at a flow rate higher than the critical one may be responsible for the hyperbolic profile of chemostat curves. The above hypothesis was confirmed by experiments conducted under extreme conditions, in which the composition of the growth medium was changed and the temperature was raised so that the cloned protein synthesis was depressed. Under such conditions, the enzyme composition of the cell was fixed and determined by the physiological state of the culture at a time when the temperature was elevated. The hypothesis was also supported by the results obtained in growing the strain with the induced protein synthesis in media to which various compounds were added.
D003470:Culture Media / Q000378:metabolism; D004262:DNA Restriction Enzymes / Q000235:genetics; D015252:Deoxyribonucleases, Type II Site-Specific; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D005798:Genes, Bacterial; D005947:Glucose / Q000378:metabolism; D007700:Kinetics; D011995:Recombination, Genetic
D003470:Culture Media; D004262:DNA Restriction Enzymes; D015252:Deoxyribonucleases, Type II Site-Specific; C027440:GATATC-specific type II deoxyribonucleases; D005947:Glucose
O mekhanizme limitirovaniia rosta rekombinantnogo shtamma Escherichia coli.