Immunolabeling of lipopolysaccharide liberated from antibiotic-treated Escherichia coli.
Increased anti-core glycolipid antibody binding was visualized by immunoelectron microscopy after incubation of Escherichia coli K1:O7 cells with a bacteriolytic antibiotic and compared with binding in control cells. The findings suggest that the core glycolipid regions of the lipopolysaccharides of some gram-negative bacilli can be effectively sequestered until liberated by antibiotic-induced cell lysis.
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000911:Antibodies, Monoclonal / Q000276:immunology; D004731:Endotoxins / Q000276:immunology*; D004926:Escherichia coli / Q000187:drug effects / Q000276:immunology / Q000648:ultrastructure*; D007150:Immunohistochemistry; D008070:Lipopolysaccharides / Q000276:immunology*; D008854:Microscopy, Electron
D000900:Anti-Bacterial Agents; D000911:Antibodies, Monoclonal; D004731:Endotoxins; D008070:Lipopolysaccharides