[The medium for determining the gelatinase activity of non-fermenting gram-negative microorganisms].
To determine the gelatinase activity of Gram-negative nonfermenting microorganisms, a double-layer medium containing 2% of agar in the lower layer and 10-20% of gelatin in the upper layer has been developed. The medium ensures free access of atmospheric oxygen, possibility of rapid (24-42 h) determination of gelatinase activity at 22-25 degrees C, efficacy of the test. simultaneous determination of gelatin hydrolysis by 8-12 strains in one dish.
D000150:Acinetobacter / Q000201:enzymology; D001431:Bacteriological Techniques; D003470:Culture Media; D005285:Fermentation; D018093:Gelatinases; D006090:Gram-Negative Bacteria / Q000201:enzymology*; D010434:Pepsin A / Q000032:analysis*
D003470:Culture Media; D010434:Pepsin A; D018093:Gelatinases
Sreda dlia opredeleniia zhelatinaznoĭ aktivnosti nefermentiruiushchikh gramotritsatel'nykh mikroorganizmov.