Retroviral vector gene expression in F9 embryonal carcinoma cells.
When F9 embryonal carcinoma (EC) cells are infected with retroviral vectors, the efficiency of expression of selectable genes is considerably lower than that in mouse fibroblasts infected with the same retroviral vectors. In this study, several retroviral vectors with regulatory sequences placed immediately 5' to a selectable gene were constructed, packaged, and used to infect mouse fibroblasts and F9 EC cells. With selection as an assay, there was a hierarchy of relative expression in F9 cells compared with that in mouse fibroblasts. These internally placed regulatory sequences are the source of the mRNAs detected in F9 EC cells, while both retroviral long-terminal-repeat promoters and internal promoters are the source of steady-state mRNAs in mouse fibroblasts. This effect was observable with both the internally placed herpes simplex virus thymidine kinase promoter and the Moloney murine leukemia virus promoter.
D000818:Animals; D002460:Cell Line; D054278:Embryonal Carcinoma Stem Cells; D004742:Enhancer Elements, Genetic; D005347:Fibroblasts; D005786:Gene Expression Regulation; D005822:Genetic Vectors; D008979:Moloney murine leukemia virus / Q000235:genetics; D008980:Moloney murine sarcoma virus / Q000235:genetics; D014411:Neoplastic Stem Cells / Q000382:microbiology*; D009693:Nucleic Acid Hybridization; D011401:Promoter Regions, Genetic; D012367:RNA, Viral / Q000032:analysis; D012091:Repetitive Sequences, Nucleic Acid; D012190:Retroviridae / Q000235:genetics*; D018139:Simplexvirus / Q000235:genetics; D013937:Thymidine Kinase / Q000235:genetics; D014158:Transcription, Genetic
D012367:RNA, Viral; D013937:Thymidine Kinase