Biologic and molecular characterization of two newly isolated ras-containing murine leukemia viruses.
A murine sarcoma virus (MSV) was recovered from an (NFS X NS.C58v-1) F1 mouse which developed splenic sarcoma and erythroleukemia 6 months after inoculation with a mink cell focus-inducing murine leukemia virus (MuLV) isolated from an NFS mouse infected with a wild mouse ecotropic MuLV. The MSV, designated NS.C58 MSV-1, induced foci of transformation in mouse and rat fibroblasts, and inoculation of mice of various strains 2 weeks of age or younger resulted in erythroleukemia and sarcomatous lesions in spleen, lymph node, and brain. The MSV provirus was molecularly cloned from a genomic library prepared from transformed non-producer rat cells. The 8.8-kilobase proviral DNA contained a 1.0-kilobase p21 ras coding segment which replaced most of the gp70-encoding portion of an MuLV, most likely the endogenous C58v-1 ecotropic virus. The ras oncogene is closely related to v-Ha-ras by hybridization, expression of p21 protein, and nucleotide sequence. It is nearly identical in sequence to v-bas, the only previously described transduced, activated mouse c-ras. At position 12 in the p21 coding region, arginine is substituted for the naturally occurring glycine present in c-ras. A second MSV isolate is described which is similar to NS.C58 MSV-1 except for a 100- to 200-base-pair deletion in the noncoding region of the ras-containing insert.
D000818:Animals; D001932:Brain Neoplasms / Q000382:microbiology; D002472:Cell Transformation, Viral; D005814:Genes, Viral; D006378:Helper Viruses / Q000235:genetics / Q000302:isolation & purification*; D006394:Hemangiosarcoma / Q000382:microbiology*; D009052:Leukemia Virus, Murine / Q000235:genetics / Q000302:isolation & purification*; D004915:Leukemia, Erythroblastic, Acute / Q000382:microbiology; D008228:Lymphoma, Non-Hodgkin / Q000382:microbiology; D051379:Mice; D008815:Mice, Inbred Strains / Q000382:microbiology; D008908:Mink Cell Focus-Inducing Viruses / Q000302:isolation & purification; D015689:Oncogene Protein p21(ras); D009856:Oncogene Proteins, Viral / Q000235:genetics; D009857:Oncogenes; D009053:Sarcoma Viruses, Murine / Q000235:genetics / Q000302:isolation & purification*; D012689:Sequence Homology, Nucleic Acid; D013160:Splenic Neoplasms / Q000382:microbiology*; D014161:Transduction, Genetic
D009856:Oncogene Proteins, Viral; D015689:Oncogene Protein p21(ras)