[Isolation and immunoenzyme determination of human beta 2-microglobulin].
Two procedures for isolation of homogeneous beta 2-microglobulin from urine of patients with systemic lupus erythematosus were developed: a procedure for isolation of beta 2-microglobulin with two-stage gel chromatography on Sephacryl S-200 and a procedure for isolation of homogeneous beta 2-microglobulin with affinity chromatography on rabbit polyclonal antibodies. The microglobulins isolated with the two procedures showed identical physicochemical properties and were used in development of a competitive immunoenzymatic assay method for determination of beta 2-microglobulin in the blood. The method was approved for determination of beta 2-microglobulin in blood serum of patients.
D000818:Animals; D000906:Antibodies / Q000302:isolation & purification; D000915:Antibody Affinity; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007114:Immunization; D007124:Immunoenzyme Techniques; D007525:Isoelectric Focusing; D008180:Lupus Erythematosus, Systemic / Q000097:blood; D008970:Molecular Weight; D011817:Rabbits; D001613:beta 2-Microglobulin / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification*
D000906:Antibodies; D001613:beta 2-Microglobulin
Poluchenie i immunofermentnoe opredelenie beta 2-mikroglobulina cheloveka.