title: Insertion element IS1 can generate a 10-base pair target duplication.
abstract: Transposable element IS1 is known to generate mainly 9-bp and occasionally 8-bp target duplications upon transposition. We have isolated a plasmid pBR322 derivative having IS1 inserted into a site between the promoter and the structural gene for tetracycline resistance. DNA sequence analysis revealed that integration of this IS1 resulted in a 10-bp target duplication.
mesh_terms: D001482:Base Composition; D001483:Base Sequence; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics*; D010957:Plasmids
chemical_list: D004251:DNA Transposable Elements