title: Transposition studies of mini-Mu plasmids constructed from the chemically synthesized ends of bacteriophage Mu.
abstract: We describe below the chemical synthesis of the right and left ends of bacteriophage Mu and characterize the activity of these synthetic ends in mini-Mu transposition. Mini-Mu plasmids were constructed which carry the synthetic Mu ends together with the Mu A and B genes under control of the bacteriophage lambda pL promoter. Derepression of pL leads to a high frequency of mini-Mu transposition (5.6 X 10(-2) which is dependent on the presence of the Mu ends and the Mu A and B proteins. Five deletion mutants in the Mu ends were tested in the mini-Mu transposition system and their effects on transposition are described.
mesh_terms: D010583:Bacteriophage mu / Q000235:genetics*; D003001:Cloning, Molecular; D003227:Conjugation, Genetic; D004251:DNA Transposable Elements; D004279:DNA, Viral / Q000138:chemical synthesis / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics; D005796:Genes; D010957:Plasmids; D011995:Recombination, Genetic; D014764:Viral Proteins / Q000235:genetics
chemical_list: D004251:DNA Transposable Elements; D004279:DNA, Viral; D004268:DNA-Binding Proteins; D014764:Viral Proteins