Cloning of the riboB locus of Aspergillus nidulans.
We have complemented the riboB2 mutation of Aspergillus nidulans by transformation with a plasmid library of wild-type (wt) sequences. We have isolated, by marker rescue from a riboB+ transformant, a plasmid that complements riboB2 efficiently. From this plasmid we have subcloned an A. nidulans sequence that complements riboB2 efficiently and that integrates by homologous recombination at a site closely linked to the riboB locus. We conclude that this sequence contains the wt riboB+ allele.
D001233:Aspergillus nidulans / Q000235:genetics*; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D005796:Genes; D005800:Genes, Fungal; D005816:Genetic Complementation Test; D009154:Mutation; D011995:Recombination, Genetic; D012256:Riboflavin / Q000378:metabolism*
D004262:DNA Restriction Enzymes; D012256:Riboflavin