title: Effect of cytolytic infection on maintenance of resistance to HVJ (Sendai virus) in an altered BHK cell culture.
abstract: Altered baby hamster kidney (BHK-R) cells were serially cultured in the continuous presence of hemagglutinating virus of Japan (HVJ). These cells showed a distinct resistance to superinfection with the homologous HVJ. This resistance of BHK-R cells gradually disappeared after serial passages in the presence of ultraviolet-irradiated HVJ particles which lost infectivity but still preserved hemagglutinating and neuraminidase activities. When BHK-R cells were serially cultured in the presence of a temperature-sensitive mutant of HVJ at non-permissive temperature, the cells also lost the resistance. The resistance of BHK-R cells remained unchanged, even after prolonged incubation in virus-free maintenance medium under the conditions of no cell division. It was suggested that killing of virus-sensitive cells, which were generated during cell proliferation, was required for maintenance of the resistance.
mesh_terms: D000818:Animals; D002455:Cell Division; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D005455:Fluorescent Antibody Technique; D006387:Hemagglutination, Viral; D009154:Mutation; D009439:Neuraminidase / Q000378:metabolism; D010222:Parainfluenza Virus 1, Human / Q000201:enzymology / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D013696:Temperature
chemical_list: D009439:Neuraminidase