Effect of platelet-activating factor on beta-adrenoceptors in human lung.
We examined the effect of platelet-activating factor (PAF) on beta-adrenoceptors in the membranes of human lung and the functional responses to methacholine, histamine and isoproterenol in human lung parenchyma and tracheal strips. Preincubation of the human lung slices with 0.1 microM PAF decreased the density of beta-adrenoceptors without a change in the affinity. In the functional studies, a subthreshold dose of PAF (0.1 microM) significantly reduced the potency of isoproterenol to reverse methacholine or histamine-induced contraction. BN 52021, a PAF antagonist, protected against these PAF-induced changes. These findings suggest that PAF-induced down-regulation of beta-adrenoceptors could be a mechanism of the non-specific airway hyperreactivity in asthma.
D002462:Cell Membrane / Q000378:metabolism; D004082:Dihydroalprenolol / Q000378:metabolism; D006632:Histamine / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D007545:Isoproterenol / Q000494:pharmacology; D007700:Kinetics; D008168:Lung / Q000187:drug effects / Q000502:physiology*; D016210:Methacholine Chloride; D008688:Methacholine Compounds / Q000494:pharmacology; D010972:Platelet Activating Factor / Q000494:pharmacology*; D011943:Receptors, Adrenergic, beta / Q000187:drug effects / Q000378:metabolism*; D014132:Trachea / Q000187:drug effects / Q000502:physiology
D008688:Methacholine Compounds; D010972:Platelet Activating Factor; D011943:Receptors, Adrenergic, beta; D016210:Methacholine Chloride; D004082:Dihydroalprenolol; D006632:Histamine; D007545:Isoproterenol