Molecular cloning of infectious proviral genomes of bovine leukemia virus.
Covalently closed circular DNA molecules of bovine leukemia virus were cloned in the lambda phage vector lambda gtWES-lambda B and subsequently in the plasmid vector pUC12. Proviral DNAs of 8.3 kb which have one copy of a long terminal repeat and uniform restriction endonuclease sites were preferentially obtained. Four randomly selected clones were examined for their biological activities by DNA transfection experiments. The ovine embryonic cells transfected with these clones formed syncytia which represent the expression of BLV genes. The infectious virions could be recovered from the transfectants.
D000818:Animals; D002417:Cattle; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000235:genetics; D001909:Leukemia Virus, Bovine / Q000235:genetics*; D012190:Retroviridae / Q000235:genetics*; D012756:Sheep; D014779:Virus Replication
D004279:DNA, Viral; D004262:DNA Restriction Enzymes