Lanthanide chelates as new fluorochrome labels for cytochemistry.
Anti-rabbit IgG labeled with a new fluorescent europium chelate was used to localize rabbit IgG to human smooth muscle myosin in a histological section. The antibody labeled with the europium chelate could be viewed with a conventional fluorescence microscope with a steady-state light source. This result encourages the development of a time-resolved fluorescence microscope, because a significant improvement in the signal-to-noise ratio can be anticipated.
D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes; D006801:Humans; D007150:Immunohistochemistry; D008856:Microscopy, Fluorescence; D009130:Muscle, Smooth / Q000032:analysis*; D009218:Myosins / Q000032:analysis*
D005456:Fluorescent Dyes; D009218:Myosins