Production and characterization of the neutralization antigen VP2 of bluetongue virus serotype 10 using a baculovirus expression vector.
DNA representing RNA segment 2 of bluetongue virus (BTV) serotype 10, corresponding to the gene that codes for the BTV neutralization antigen VP2, has been inserted into a baculovirus transfer vector in lieu of the 5' coding region of the polyhedrin gene of Autographa californica nuclear polyhedrosis virus (AcNPV). After cotransfection of Spodoptera frugiperda cells with wild-type AcNPV DNA in the presence of the derived recombinant transfer vector DNA, polyhedrin-negative recombinant baculoviruses were recovered. When S. frugiperda cells were infected with one of these recombinant viruses, a protein that was similar in size and antigenic properties to the BTV VP2 protein was synthesized. Antibodies raised in mice or rabbits to the baculovirus expressed VP2 protein neutralized the infectivity of BTV-10 virus and to lesser extents BTV serotype 11 and 17 viruses but not BTV-13 virus.
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D000956:Antigens, Viral / Q000235:genetics* / Q000276:immunology; D001820:Bluetongue virus / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D002460:Cell Line; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant; D004279:DNA, Viral / Q000235:genetics; D005814:Genes, Viral; D005822:Genetic Vectors; D007304:Insect Viruses / Q000235:genetics* / Q000276:immunology; D051379:Mice; D009036:Moths; D009500:Neutralization Tests; D009693:Nucleic Acid Hybridization; D012087:Reoviridae / Q000235:genetics*; D014162:Transfection; D014764:Viral Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D015678:Viral Structural Proteins
D000914:Antibodies, Viral; D000956:Antigens, Viral; D004274:DNA, Recombinant; D004279:DNA, Viral; D014764:Viral Proteins; D015678:Viral Structural Proteins; D004262:DNA Restriction Enzymes