Structure of the DNA gyrase-DNA complex as revealed by transient electric dichroism.
We have analyzed the structure of complexes between DNA gyrase and four defined DNA fragments by electric dichroism. Both the extrapolated dichroism and relaxation time of these complexes suggest that a single turn of DNA is wrapped around the enzyme with the entry and exit points located close together. The average angle between the DNA tails emerging from the particle is about 120 degrees. This structure is consistent with that seen by electron microscopy. Addition of ATP or the non-hydrolyzable ATP analog 5'-adenylyl-beta, gamma-imidodiphosphate results in a structural change of the complex, consistent with the DNA tails now being wrapped around the protein. The significance of these observations with respect to the mechanism of DNA supercoiling by DNA gyrase is discussed.
D001345:Autoradiography; D002942:Circular Dichroism; D004247:DNA / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D004250:DNA Topoisomerases, Type II / Q000378:metabolism*; D008854:Microscopy, Electron; D009643:Norfloxacin / Q000494:pharmacology
D004247:DNA; D004250:DNA Topoisomerases, Type II; D009643:Norfloxacin