Proton NMR studies of two helices in tuna ferricytochrome c.
1H Nuclear magnetic resonance assignments are given for the NH and C alpha H protons of two alpha-helical segments of tuna ferricytochrome c. The assignments were obtained using two-dimensional nuclear magnetic resonance sequential assignment procedures and illustrate the applicability of these methods to medium-sized proteins. By comparing nuclear Overhauser intensities between the NH and C alpha H protons the precise structures of the two helical segments are compared and their deviations from ideality are discussed.
D000818:Animals; D003574:Cytochrome c Group; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008958:Models, Molecular; D011487:Protein Conformation; D014413:Tuna
D003574:Cytochrome c Group