Insulin receptors from guinea pig liver and brain: structural and functional studies.
We studied the structural and functional characteristics of insulin receptors from guinea pig liver and brain. Binding to crude membrane preparations of liver and brain was time, temperature, and pH dependent. Maximal specific binding to liver crude membrane preparations was 16.4 +/- 0.5%, and that to brain crude membrane preparations was 10.4 +/- 1.8%. Specificity studies demonstrated typical affinities for insulin receptors with chicken insulin greater than porcine insulin greater than human proinsulin greater than desoctapeptide insulin in both liver and brain. Antiinsulin receptor antiserum inhibited binding of [125I]insulin to both liver and brain crude membrane preparations. Electrophoresis performed under reducing conditions after affinity cross-linking of liver and brain insulin receptors with [125I]insulin revealed labeled proteins (alpha-subunit) with apparent mol wt of 136,000 in liver and 121,000 in brain. Treatment of liver and brain receptors with both endoglycosidase H and endoglycosidase F increased the electrophoretic mobility of the alpha-subunit. [125I]Insulin cross-linked receptors from both liver and brain adsorbed to and eluted from wheat germ agglutinin columns in a similar manner, as demonstrated by binding and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. In solubilized lectin-purified receptor preparations from liver and brain, insulin stimulated the phosphorylation of the beta-subunit and exogenous substrates. When the delta-kinase activity, as measured by exogenous substrate phosphorylation, of the brain and liver preparations was normalized to the maximal bound to free ratio of the preparations, the delta-kinase activity was about 4-fold greater in brain than in liver. These studies suggest that the differences between brain and liver insulin receptor alpha-subunits previously demonstrated in rats are also present in guinea pigs.
Lowe
W
W
LeRoith
D
D
D000118:Acetylglucosaminidase / Q000378:metabolism; D000818:Animals; D001923:Brain Chemistry; D002645:Chickens; D006026:Glycoside Hydrolases / Q000378:metabolism; D006168:Guinea Pigs; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007328:Insulin / Q000378:metabolism; D037102:Lectins / Q000378:metabolism; D008099:Liver / Q000032:analysis*; D046911:Macromolecular Substances; D008297:Male; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase; D008970:Molecular Weight; D011384:Proinsulin / Q000378:metabolism; D011972:Receptor, Insulin / Q000378:metabolism / Q000502:physiology*; D013329:Structure-Activity Relationship; D013552:Swine; D013696:Temperature; D013997:Time Factors; D014909:Wheat Germ Agglutinins
D007328:Insulin; D037102:Lectins; D046911:Macromolecular Substances; D014909:Wheat Germ Agglutinins; D011384:Proinsulin; D011972:Receptor, Insulin; D006026:Glycoside Hydrolases; D000118:Acetylglucosaminidase; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase