[Isolation of group-specific polysaccharide complexes from group B streptococci by phenol-water extraction].
The group specific polysaccharide of Group B streptococci was isolated either by means of phenol-water extraction or by Lancefield extraction procedures. Two groups of rabbits were immunized with these substances to induce polyclonal antibodies. The phenol-water extract induced antisera had a significantly higher titer against B streptococci than against A-, D- and G streptococci in the agglutination reaction. The B-specific titers in rabbits immunized with phenol-water extract were significantly higher than those obtained in the rabbits immunized with Lancefield extract. In counter immunoelectrophoresis, only the antisera prepared by immunization with phenol-water extract reacted with standard antigen from group B streptococci. The serological specificity of the phenol-water extract antigen isolated from B streptococci was confirmed by counterimmunoelectrophoresis. The antigen was a heterologous fraction with a molecular mass of 8 x 10(4)-2 x 10(6) Dalton.
Mehl
M
M
Institut für Medizinische Mikrobiologie des Bereiches Medizin (Charié), Humboldt-Universität zu Berlin.
Starke
R
R
Möckel
C
C
Presber
W
W
D003378:Counterimmunoelectrophoresis; D007120:Immunochemistry; D008970:Molecular Weight; D019800:Phenol; D010636:Phenols; D011135:Polysaccharides, Bacterial / Q000276:immunology / Q000302:isolation & purification*; D013292:Streptococcus agalactiae / Q000032:analysis* / Q000276:immunology; D014867:Water
D010636:Phenols; D011135:Polysaccharides, Bacterial; D014867:Water; D019800:Phenol
Einsatz der Phenol-Wasser-Extraktion zur Isolierung des gruppenspezifischen Polysaccharidkomplexes aus B-Streptokokken.