[Display of 8-hydroxy-GTP substrate properties of UTP in the reaction of polynucleotide synthesis catalyzed by RNA-polymerase from Escherichia coli in the presence of poly[d(AT).d(AT)] template].
8-oxy-GTP was obtained via reaction of GTP with ascorbic acid and addition of hydrogen peroxide. 8-oxy-GTP is recognized and displays substrate properties of UTP on substitution of 8-oxy-GTP for UTP in polynucleotide synthesis catalyzed by E. coli RNA polymerase on a poly[d(A-T)].poly[d(A-T)] template. Such incorporation does not take place at equimolar quantities of GTP and 8-Br-GTP. The incorporation of 8-oxy-GTP instead of UTP, is 2.5-3 times higher upon replacement of Mg2+ by Mn2+ ions. The dinucleotide ApU serving as an initiator rises the incorporation level of 8-oxy-GTP both for Mg2+ and Mn2+ ions. 8-oxy-GTP slightly inhibits poly[r(A-U)] synthesis, but UTP strongly inhibits the incorporation of 8-oxy-GTP. [alpha-32P] 8-oxy-GTP is incorporated mainly instead of UTP, but it can be incorporated also during the substitution of 8-oxy-GTP for ATP.
Bruskov
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Kuklina
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D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology; D006160:Guanosine Triphosphate / Q000031:analogs & derivatives* / Q000378:metabolism; D009690:Nucleic Acid Conformation; D011067:Poly dA-dT / Q000378:metabolism*; D011089:Polydeoxyribonucleotides / Q000096:biosynthesis* / Q000378:metabolism*; D013379:Substrate Specificity; D013698:Templates, Genetic; D014500:Uracil Nucleotides / Q000378:metabolism*; D014544:Uridine Triphosphate / Q000378:metabolism*
D011089:Polydeoxyribonucleotides; D014500:Uracil Nucleotides; C057674:8-hydroxyguanosine triphosphate; D011067:Poly dA-dT; D006160:Guanosine Triphosphate; D012321:DNA-Directed RNA Polymerases; D014544:Uridine Triphosphate
Proiavlenie 8-oksi-GTP substratnykh svoĭstv UTP v reaktsii polinukleotidnogo sinteza RNK-polimerazoĭ Escherichia coli na matritse poli[d(AT).d(AT)].