Modification of the DNA colony hybridization technique for multiple filter analysis.
Inexpensive fiberglass mesh window screens were used as spacers between colony blot filters to increase the number of bacterial isolates that could be tested by DNA colony hybridization. Sixty filters with up to 48 isolates per filter were tested at one time. This modified technique reduced the amount of radiolabelled probe and other materials needed for simultaneous analysis of up to 2880 test isolates.
Kaysner
C A
CA
Seattle District Office, Food and Drug Administration, Washington 98174.
Weagant
S D
SD
Hill
W E
WE
D000818:Animals; D001345:Autoradiography; D002999:Clone Cells / Q000032:analysis; D004247:DNA / Q000235:genetics*; D015342:DNA Probes / Q000032:analysis; D004926:Escherichia coli / Q000235:genetics; D008722:Methods; D009693:Nucleic Acid Hybridization; D010761:Phosphorus Radioisotopes; D010957:Plasmids; D015008:Yersinia enterocolitica / Q000235:genetics
D015342:DNA Probes; D010761:Phosphorus Radioisotopes; D004247:DNA