Isolation and characterization of Mycoplasma mycoides subsp. mycoides mutants deficient in nucleoside monophosphate transport.
To select mutants lacking dAMP uptake, log-phase cells of Mycoplasma mycoides subsp. mycoides Y were incubated with high-specific-activity [32P]dAMP and then stored several weeks at -20 degrees C to allow 32P decay before plating out. Mutants were screened for lack of labeling by [32P]dAMP. Two mutants were studied further by uptake and growth experiments with other nucleotides.
Youil
R
R
Russell Grimwade School of Biochemistry, University of Melbourne, Parkville, Victoria, Australia.
Finch
L R
LR
D001692:Biological Transport; D004269:DNA, Bacterial / Q000096:biosynthesis; D003838:Deoxyadenine Nucleotides / Q000378:metabolism*; D003854:Deoxyribonucleotides / Q000378:metabolism; D007700:Kinetics; D009154:Mutation; D009176:Mycoplasma mycoides / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D012265:Ribonucleotides / Q000378:metabolism
D004269:DNA, Bacterial; D003838:Deoxyadenine Nucleotides; D003854:Deoxyribonucleotides; D012265:Ribonucleotides; C012873:2'-deoxy-5'-adenosine monophosphate