title: Cyclo (His-Pro), a metabolite of thyrotropin-releasing hormone: specific binding to rat liver membranes.
abstract: [3H]cyclo(His-Pro) bound with high affinity (59 nM) to a single class of sites in rat liver plasma membranes, without significant tracer degradation during equilibration for 60 min at 0 degrees C. Binding was specific and saturable (3.9 pmol/mg protein), and were increased by the addition of K+, Mg++ and Na+ at optimal concentrations, but not of Ca++ at all concentrations tested. In vivo administration of cyclo(His-Pro), but not thyrotropin-releasing hormone, to rats caused the downregulation of cyclo(His-Pro)-binding sites with decreases in specific binding numbers but did not change binding affinity.
authors.forename: M
authors.initials: M
authors.lastname: Mori
authors.forename: M
authors.initials: M
authors.lastname: Yamada
authors.forename: M
authors.initials: M
authors.lastname: Yamaguchi
authors.forename: M
authors.initials: M
authors.lastname: Suzuki
authors.forename: K
authors.initials: K
authors.lastname: Ohshima
authors.forename: I
authors.initials: I
authors.lastname: Kobayashi
authors.forename: S
authors.initials: S
authors.lastname: Kobayashi
mesh_terms: D000818:Animals; D001665:Binding Sites; D002118:Calcium / Q000378:metabolism; D003080:Cold Temperature; D008099:Liver / Q000378:metabolism*; D008274:Magnesium / Q000378:metabolism; D008297:Male; D008566:Membranes / Q000378:metabolism; D010456:Peptides, Cyclic / Q000378:metabolism*; D010879:Piperazines / Q000378:metabolism*; D011188:Potassium / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012964:Sodium / Q000378:metabolism; D013973:Thyrotropin-Releasing Hormone / Q000378:metabolism*; D013997:Time Factors
chemical_list: D010456:Peptides, Cyclic; D010879:Piperazines; D013973:Thyrotropin-Releasing Hormone; D012964:Sodium; D008274:Magnesium; C015924:histidyl-proline diketopiperazine; D011188:Potassium; D002118:Calcium