The human c-fos serum response factor and the yeast factors GRM/PRTF have related DNA-binding specificities.
One of the elements that mediates growth factor and serum inducibility of the human c-fos gene is a region of dyad symmetry that lies between nucleotides -320 and -299 of the human gene. A mammalian protein specifically binds to this sequence element and has been termed the serum response factor (SRF). Gel-shift analysis and competition experiments demonstrate that there is a factor in the yeast Saccharomyces cerevisiae that binds specifically to the human c-fos SRE. The methylation interference pattern of the yeast factor is identical to that of the mammalian SRF. Regulatory elements of cell-type-specific genes in yeast have homologies to the c-fos SRE and complete for binding of both the mammalian and yeast factors to the SRE. Antisera to the gene product of the MCM1 locus react with the yeast SRE-binding factor. These data suggest that this yeast protein is closely related or identical to the factors [general regulator of mating type (GRM) and pheromone/receptor transcription factor (PRTF)] that are required for the regulation of cell-type-specific genes in yeast.
Hayes
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Center for Cancer Research, Massachusetts Institute of Technology, Cambridge 02139.
Sengupta
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Cochran
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D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics; D004742:Enhancer Elements, Genetic; D005656:Fungal Proteins / Q000235:genetics*; D005800:Genes, Fungal; D049770:Genes, Mating Type, Fungal; D006801:Humans; D008745:Methylation; D009687:Nuclear Proteins / Q000235:genetics*; D015345:Oligonucleotide Probes; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D026362:Serum Response Factor; D013045:Species Specificity; D014157:Transcription Factors / Q000235:genetics*; D014170:Transformation, Genetic
D004271:DNA, Fungal; D005656:Fungal Proteins; D009687:Nuclear Proteins; D015345:Oligonucleotide Probes; D026362:Serum Response Factor; D014157:Transcription Factors; D004247:DNA