[Temperature-dependent structural changes in complexes of tRNA(adenine-1)-methyltransferase from Thermus thermophilus HB8 and tRNA studied by optical and electron microscope methods].
The complexation of tRNA (adenine-1-)-methyltransferase from Thermus thermophilus HB8 (E.C.2.1.1.36) with Escherichia coli tRNA(Phe) and yeast tRNA1(Val) was investigated in a temperature range from 20 to 90 degrees C. The quantity of methylase subunits bounded with tRNA and the association constant (Ka) were determined by means of fluorescence quenching of the enzyme tryptophane residues by tRNA molecules. The number of enzyme subunits bounded with one tRNA molecule at temperatures 20-70 degrees C is equal to 8 +/- 2. The Ka values increase from (2 divided by 3).10(7) at 20 degrees C up to 8.5.10(7) M-1 at 70 degrees C. The temperature increase from 70 to 90 degrees C causes a decrease in the enzyme specific activity and in Ka values. In the temperature range from 75 to 90 degrees C a cooperative transition of methylase macromolecules into associates was observed. This association is accompanied by an increase of UV-light scattering and of fluorescence polarization coefficient of methylase tryptophane residues. In the absence of tRNA the size of enzyme associates (d) is evaluated to be more than 320 nm (d greater than or equal to lambda-320 nm), in the presence of tRNA-less than 320 nm (d much less than lambda-320 nm). An electron microscopic investigation of methylase and its complexes with tRNA at 20 degrees C revealed disk-like particles with a diameter and height of 8-11 nm and 4-5 nm, respectively. These disk-like methylase preparations dialized against distilled water form flexible polymeric rods with a diameter of 10-12 nm and the length of about several hundreds nm. During complexation of methylase with tRNA, in the same conditions as the dializes was carried out, large associates were not revealed.
Borisova
O F
OF
Kuznetsova
N V
NV
Morozov
I A
IA
Beliaeva
N N
NN
Venkstern
T V
TV
D004926:Escherichia coli / Q000378:metabolism; D008854:Microscopy, Electron; D012345:RNA, Transfer, Amino Acid-Specific / Q000378:metabolism*; D012360:RNA, Transfer, Phe / Q000378:metabolism*; D012366:RNA, Transfer, Val / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013379:Substrate Specificity; D013696:Temperature; D013824:Thermus / Q000201:enzymology*; D012359:tRNA Methyltransferases / Q000378:metabolism*
D012345:RNA, Transfer, Amino Acid-Specific; D012360:RNA, Transfer, Phe; D012366:RNA, Transfer, Val; D012359:tRNA Methyltransferases; C023194:tRNA (adenine(58)-N(1))-methyltransferase
Temperaturozavisimye strukturnye izmeneniia v kompleksakh tRNK(adenin-1)-metiltransferazy iz Thermus thermophilus HB8 i tRNK, issledovannye opticheskimi i élektronno-mikroskopicheskimi metodami.