title: The effect of human recombinant GM-CSF on the myelopoiesis in a long-term bone marrow culture (LTBMC).
abstract: The long-term bone marrow culture (LTBMC) allows continuous self-renewal of a myeloid precursor population from an adherent stromal cell layer. We investigated the effect of human recombinant GM-CSF (100 ng/ml) on the in vitro hemopoiesis in a LTBMC. After a culture period of 14 days we observed a 2.3-fold increase in the total number of non-adherent cells in the cultures containing GM-CSF. Whereas the total cell number in the cultures without GM-CSF decreased after 28 days, proliferation continued in the cultures with GM-CSF. During the first 14 days there was a slight decrease of the number of CFU-GM in the cultures with GM-CSF. Thereafter no significant difference in the numbers of committed progenitors present in two culture systems could be observed.
authors.affiliation: Department of Internal Medicine, Hematology/Oncology and Rheumatology, University of Heidelberg, W.-Germany.
authors.forename: R
authors.initials: R
authors.lastname: Haas
authors.forename: S
authors.initials: S
authors.lastname: Kiesel
authors.forename: E
authors.initials: E
authors.lastname: Ogniben
authors.forename: M
authors.initials: M
authors.lastname: Baumann
authors.forename: S
authors.initials: S
authors.lastname: Hohaus
authors.forename: M
authors.initials: M
authors.lastname: Körbling
authors.forename: B
authors.initials: B
authors.lastname: Dörken
authors.forename: W
authors.initials: W
authors.lastname: Hunstein
mesh_terms: D001854:Bone Marrow Cells; D002478:Cells, Cultured; D003115:Colony-Stimulating Factors / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D006133:Growth Substances / Q000502:physiology*; D006410:Hematopoiesis / Q000187:drug effects; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000187:drug effects; D006801:Humans; D011994:Recombinant Proteins / Q000494:pharmacology*; D012016:Reference Values
chemical_list: D003115:Colony-Stimulating Factors; D006133:Growth Substances; D011994:Recombinant Proteins; D016178:Granulocyte-Macrophage Colony-Stimulating Factor