Reciprocality of recombination events that rearrange the chromosome.
We describe a genetic system for studying the reciprocality of chromosomal recombination; all substrates and recombination functions involved are provided exclusively by the bacterial chromosome. The genetic system allows the recovery of both recombinant products from a single recombination event. The system was used to demonstrate the full reciprocality of three different types of recombination events: (1) intrachromosomal recombination between direct repeats, causing deletions; (2) intrachromosomal recombination between inverse homologies, causing inversion of a segment of the bacterial chromosome; and (3) circle to circle recombination (in the absence of any plasmid or phage functions). Results suggest that intrachromosomal recombination in bacteria is frequently fully reciprocal.
Mahan
M J
MJ
Department of Biology, University of Utah, Salt Lake City 84112.
Roth
J R
JR
D002876:Chromosomes, Bacterial / Q000502:physiology*; D005798:Genes, Bacterial; D008957:Models, Genetic; D011995:Recombination, Genetic; D012486:Salmonella typhimurium / Q000235:genetics*; D014161:Transduction, Genetic