High-level resistance to trimethoprim in Shigella sonnei associated with plasmid-encoded dihydrofolate reductase type I.
By DNA hybridization, the gene encoding dihydrofolate reductase type I was found in 58 of 59 highly trimethoprim-resistant clinical isolates of Shigella sonnei obtained from 1981 through 1987 in Madrid, Spain. No strain harbored the type II gene. In selected strains, the type I gene was demonstrated to be in a plasmid.
Delgado
R
R
Servicio de Microbiología, Hospital Primero de Octubre, Madrid, Spain.
Otero
J R
JR
D009693:Nucleic Acid Hybridization; D011815:R Factors; D012764:Shigella sonnei / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics*; D014296:Trimethoprim Resistance / Q000235:genetics*
D013762:Tetrahydrofolate Dehydrogenase