title: Role of plasma membrane and of cytomatrix in maintenance of intracellular to extracellular ion gradients in chicken erythrocytes.
abstract: Ultrastructural observations in combination with electron probe X-ray microanalysis on detergent (Brij 58) permeabilized (disruption of the plasma membrane) nucleated chicken erythrocytes support the view that a large fraction of cytoplasmic and nuclear K+ is not freely diffusible and that adsorption of K+ on detergent released mobilizable proteins exists within the cell. The data also suggest that the detergent proteins are normally immobilized by a detergent-resistant cytoskeleton so that they are not immediately free to diffuse from the cell for several minutes after detergent disruption of the plasma membrane.
authors.affiliation: University of Texas Health Science Center, San Antonio 78284.
authors.forename: I L
authors.initials: IL
authors.lastname: Cameron
authors.forename: K E
authors.initials: KE
authors.lastname: Hunter
authors.forename: N K
authors.initials: NK
authors.lastname: Smith
authors.forename: C F
authors.initials: CF
authors.lastname: Hazlewood
authors.forename: A
authors.initials: A
authors.lastname: Ludany
authors.forename: M
authors.initials: M
authors.lastname: Kellermayer
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000502:physiology*; D002645:Chickens; D004577:Electron Probe Microanalysis; D004912:Erythrocytes / Q000166:cytology*; D005109:Extracellular Matrix / Q000502:physiology*; D008854:Microscopy, Electron; D008858:Microscopy, Phase-Contrast