Qualitative dissimilarities of insulin and proinsulin binding and action in vitro at IM 9-lymphocytes.
To prove hormone analogy of insulin and proinsulin 1. biochemical characterization of binding, 2. competition-inhibition-assay, 3. up-or-down-regulation, and 4. 3H-thymidine incorporation in IM 9-lymphocytes in vitro were applied. Marked differences in binding and biological activity of insulin and proinsulin in the physiological range (10 pM-1 nM) are obvious. Heterogeneity of specific insulin and proinsulin binding sites is suggested.
Rausch
U W
UW
Innere Medizin Abteilung II, Universität Ulm, Germany.
Fussgänger
R D
RD
Heinze
E
E
D001667:Binding, Competitive; D002452:Cell Count; D002460:Cell Line; D004247:DNA / Q000096:biosynthesis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007328:Insulin / Q000378:metabolism* / Q000494:pharmacology; D007457:Iodine Radioisotopes; D007700:Kinetics; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D011384:Proinsulin / Q000378:metabolism* / Q000494:pharmacology; D013696:Temperature
D007328:Insulin; D007457:Iodine Radioisotopes; D004247:DNA; D011384:Proinsulin