Mitochondrial ribosomes of yeast: isolation of individual proteins and N-terminal sequencing.
Proteins of the small and large subunits of mitochondrial ribosomes from the yeast Saccharomyces cerevisiae were isolated and characterized by two-dimensional gel electrophoresis. Ribosomal proteins of the large subunit were separated by reverse-phase HPLC and up to 37 amino acid residues of the N-terminal sequences of L3, L4, L9 and L31 were determined. No significant homology to ribosomal protein sequences so far determined from other organisms was found.
Graack
H R
HR
Max-Planck-Institut für Molekulare Genetik, Berlin, Germany.
Grohmann
L
L
Choli
T
T
D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D008928:Mitochondria / Q000648:ultrastructure*; D008969:Molecular Sequence Data; D012269:Ribosomal Proteins / Q000302:isolation & purification*; D012270:Ribosomes / Q000032:analysis*; D012441:Saccharomyces cerevisiae / Q000648:ultrastructure*; D012689:Sequence Homology, Nucleic Acid
D012269:Ribosomal Proteins