Modulation of allotransplantation tolerance induction by interleukin-1 and interleukin-2.
Transplantation tolerance was induced in mice by inoculating newborn animals with semi-allogeneic haematopoietic cells. The mice rendered tolerant were treated within the first week of birth, or at the time of grafting (age 7-8 weeks), with recombinant interleukin-1 (rIL-1) or interleukin-2 (rIL-2). The effects of these treatments on tolerance induction were monitored in terms of skin allograft survival. Treatment of newborn mice with rIL-2 abolished tolerance induction in nearly all tested animals. When administered at the time of grafting, both rIL-1 and rIL-2 decreased the proportion of tolerant animals. However, these modulation effects of interleukins were only observed in strain combinations with genetic differences at the K end of H-2 or in the entire H-2 complex, in which it is difficult to establish permanent tolerance; no effects of interleukins on tolerance induction were found in a strain combination with a relatively weaker genetic barrier represented by incompatibility at the D region of the H-2 complex.
Holán
V
V
Institute of Molecular Genetics, Czechoslovak Academy of Sciences, Prague.
D000818:Animals; D016026:Bone Marrow Transplantation; D005260:Female; D006183:H-2 Antigens / Q000235:genetics; D007108:Immune Tolerance; D007375:Interleukin-1 / Q000494:pharmacology*; D007376:Interleukin-2 / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D016038:Skin Transplantation; D013154:Spleen / Q000637:transplantation; D014184:Transplantation, Homologous
D006183:H-2 Antigens; D007375:Interleukin-1; D007376:Interleukin-2