Regulation of renin-like enzyme in cultured human vascular smooth muscle cells.
Cultured human arterial smooth muscle cells produced an immunologically specific renin-like enzyme. The renin-like enzyme in the culture medium was mostly an inactive form; the proportion of the active form in the cell was 30 to 75%. Phorbol 12-myristate 13-acetate, N'-O'-dibutylyladenosine 3', 5'-monophosphate and isoproterenol with theophylline increased the renin-like enzyme in the medium and in the cell, dose dependently. Endothelial cell growth supplement also increased the renin-like enzyme produced by cultured vascular smooth muscle cells, and heparin promoted the effects of endothelial cell growth supplement. The existence of the regulation of the renin-like enzyme produced by cultured vascular smooth muscle cells strongly suggests the existence of a local renin angiotensin system in human vascular walls.
Iwai
N
N
Department of Internal Medicine, Kyoto University, Japan.
Matsunaga
M
M
Kita
T
T
Tei
M
M
Kawai
C
C
D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology; D004792:Enzyme Precursors / Q000502:physiology; D006493:Heparin / Q000494:pharmacology; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology; D007545:Isoproterenol / Q000494:pharmacology; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000201:enzymology*; D012083:Renin / Q000096:biosynthesis / Q000502:physiology*; D012084:Renin-Angiotensin System; D013806:Theophylline / Q000494:pharmacology; D000092502:Renin Inhibitors
D004792:Enzyme Precursors; D006493:Heparin; D007106:Immune Sera; D007545:Isoproterenol; D012083:Renin; D013806:Theophylline; D000092502:Renin Inhibitors