Immunogenic protein MPB57 from Mycobacterium bovis BCG: molecular cloning, nucleotide sequence and expression.
Using a single-probe method, we have cloned the gene for an immunogenic MPB57 protein of Mycobacterium bovis BCG. The nucleotide sequence includes an ORF of 300 base pairs encoding a protein of 99 amino acids with an Mr of 10,818. This cloned gene was expressed in an Escherichia coli expression vector to give a mature protein which reacted with a polyclonal antibody raised against MPB57.
Yamaguchi
R
R
Central Research Laboratories, Ajinomoto Co. Inc., Kawasaki, Japan.
Matsuo
K
K
Yamazaki
A
A
Nagai
S
S
Terasaka
K
K
Yamada
T
T
D000595:Amino Acid Sequence; D000942:Antigens, Bacterial / Q000235:genetics* / Q000276:immunology; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D003062:Codon; D005786:Gene Expression Regulation; D015151:Immunoblotting; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009163:Mycobacterium bovis / Q000235:genetics* / Q000276:immunology; D014614:Vaccines, Synthetic
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D003062:Codon; D014614:Vaccines, Synthetic