Generation and preliminary characterization of three mouse monoclonal antibodies reacting with human B lymphocytes.
The paper describes generation and characterization of three monoclonal antibodies (BR3, BR16, BR31) specific for human B lymphocytes. BR3 and BR31 stain highly purified population of B lymphocytes but fail to react with granulocytes, monocytes and E+ lymphocytes. BR16 in addition to B cells also stains 10% peripheral T cells and 70% granulocytes. BR3 staining pattern closely correlates that obtained with antihuman Ig both in peripheral blood and in lymphoid tissues. BR31 recognizes a subpopulation of B lymphocytes (about 50%).
Skibiński
G
G
Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław.
Wieczorek
Z
Z
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000096:biosynthesis*; D000918:Antibody Specificity; D001402:B-Lymphocytes / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C
D000911:Antibodies, Monoclonal