Detection of antigenic variation among strains of Mycoplasma gallisepticum by enzyme-linked immunosorbent inhibition assay (ELISIA) and Western blot analysis.
Polyclonal antisera (PCA) to three Mycoplasma gallisepticum (MG) strains (F, S6, and A5969) produced in rabbits were used in enzyme-linked immunosorbent inhibition assay (ELISIA) and Western blot analysis to examine antigenic variability among these strains of MG. In ELISIA, inhibiting antigen of the same strain used for immunization always led to the greatest percentage inhibition of PCA reactivity. Western blot analysis of antigens of four MG strains demonstrated both common and restricted patterns of immune recognition among the three PCAs.
Thomas
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CB
Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison 53706.
Sharp
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D000818:Animals; D000940:Antigenic Variation; D000942:Antigens, Bacterial / Q000032:analysis*; D001428:Bacterial Vaccines / Q000276:immunology; D015153:Blotting, Western; D002645:Chickens / Q000276:immunology / Q000382:microbiology; D003470:Culture Media; D003720:Densitometry; D004591:Electrophoresis, Polyacrylamide Gel; D007124:Immunoenzyme Techniques; D009174:Mycoplasma / Q000276:immunology*; D009175:Mycoplasma Infections / Q000276:immunology / Q000662:veterinary; D011201:Poultry Diseases / Q000276:immunology; D013045:Species Specificity
D000942:Antigens, Bacterial; D001428:Bacterial Vaccines; D003470:Culture Media