Production and purification of Escherichia coli hemolysin.
Eight strains of Escherichia coli, isolated from patients with a urinary tract infection were investigated for production of hemolysin. Six of these produced hemolysin and one revealed maximum hemolytic activity. Three urinary and two faecal isolates were positive for mannose-resistant hemagglutination. One isolate positive for hemagglutination and giving maximum hemolytic activity was then used. Hemolysin was present in the supernatant broth and the medium of choice to obtain the optimum yield was the alkaline meat extract broth followed by brain heart infusion broth. The highest yield appeared in the exponential phase of growth. Hemolysin is a heat-labile protein, being produced optimally at pH 8. A three-stage procedure was the best method for its purification.
Asnani
P J
PJ
Department of Microbiology, Panjab University, Chandigarh, India.
Bhatnagar
N
N
Bhandari
S
S
D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000302:isolation & purification / Q000652:urine; D004926:Escherichia coli / Q000254:growth & development / Q000378:metabolism*; D005243:Feces / Q000032:analysis / Q000382:microbiology; D006386:Hemagglutination Tests; D006460:Hemolysin Proteins / Q000096:biosynthesis* / Q000302:isolation & purification / Q000652:urine; D006801:Humans; D006863:Hydrogen-Ion Concentration
D001426:Bacterial Proteins; D006460:Hemolysin Proteins