Ultrastructural localization of secretory granule constituent chromogranin and 7 B 2.
Our immunoelectron microscopic pre-embedding method confirmed that chromogranin A (CGA) 7 B 2 and EGC were actual secretory granule constituents. The presence of these constituents in rough endoplasmic reticulum indicated their production in the tumor cells. Similar localization of CGA to 7 B 2 suggested that CGA may be secreted into the circulation as it is for 7 B 2. Different mechanism of granular formation is proposed between GH secreting adenomas and FSH secreting adenomas.
Osamura
R Y
RY
Department of Pathology, Tokai University School of Medicine, Kanagawa-ken, Japan.
Chrétien
M
M
Marcinkiewicz
M
M
D000236:Adenoma / Q000648:ultrastructure*; D053379:Chromogranin A; D002864:Chromogranins / Q000032:analysis*; D003594:Cytoplasmic Granules / Q000648:ultrastructure*; D013006:Growth Hormone / Q000032:analysis; D006801:Humans; D007124:Immunoenzyme Techniques; D008854:Microscopy, Electron; D009419:Nerve Tissue Proteins / Q000032:analysis*; D051859:Neuroendocrine Secretory Protein 7B2; D010907:Pituitary Hormones / Q000032:analysis*; D010911:Pituitary Neoplasms / Q000648:ultrastructure*; D011388:Prolactin / Q000032:analysis
D053379:Chromogranin A; D002864:Chromogranins; D009419:Nerve Tissue Proteins; D051859:Neuroendocrine Secretory Protein 7B2; D010907:Pituitary Hormones; C497678:SCG5 protein, human; D011388:Prolactin; D013006:Growth Hormone