title: Synthesis and processing of Escherichia coli TEM-beta-lactamase and Bacillus licheniformis alpha-amylase in E. coli: the role of signal peptidase I.
abstract: A mutant of Escherichia coli, in which signal peptidase I synthesis can be regulated, was constructed. The mutant was used to study the effects of signal peptidase I limitation on the synthesis and efficiency of processing of two proteins: the periplasmic E. coli TEM-beta-lactamase and Bacillus licheniformis alpha-amylase, which also accumulates in the periplasm of E. coli. Signal peptidase I limitation resulted in reduced rates of processing of pre-beta-lactamase and in strong inhibition of synthesis of alpha-amylase. The data suggest that beta-lactamase is processed post-translationally and that an intimate relationship exists between the synthesis and processing of alpha-amylase.
authors.affiliation: Department of Genetics, Center of Biological Sciences, Haren, The Netherlands.
authors.forename: J M
authors.initials: JM
authors.lastname: van Dijl
authors.forename: H
authors.initials: H
authors.lastname: Smith
authors.forename: S
authors.initials: S
authors.lastname: Bron
authors.forename: G
authors.initials: G
authors.lastname: Venema
mesh_terms: D001407:Bacillus / Q000201:enzymology* / Q000235:genetics; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000378:metabolism*; D001693:Biological Transport, Active; D003001:Cloning, Molecular; D010450:Endopeptidases / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D005786:Gene Expression Regulation; D008565:Membrane Proteins; D009154:Mutation; D010957:Plasmids; D011499:Protein Processing, Post-Translational; D015183:Restriction Mapping; D012697:Serine Endopeptidases; D000516:alpha-Amylases / Q000096:biosynthesis / Q000378:metabolism*; D001618:beta-Lactamases / Q000096:biosynthesis / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D008565:Membrane Proteins; D000516:alpha-Amylases; D010450:Endopeptidases; D012697:Serine Endopeptidases; C026515:type I signal peptidase; D001618:beta-Lactamases