Expression and synthesis of fibronectin and laminin by an intestinal epithelial cell line.
An intestinal epithelial cell line (IEC-17), undergoing a process of progressive morphological differentiation, was analysed for expression and synthesis of the extracellular matrix glycoproteins, fibronectin (FN) and laminin (LM). FN and LM cell surface expression was detected by immunoelectron microscopy, while intracytoplasmic accumulation was shown by immunofluorescence. 35S-methionine metabolic labelling was also performed to demonstrate FN and LM synthesis by IEC-17. We have compared two different maturation stages of the cell culture and have found that either early epithelial monolayer cells or later multistratified organoid structure cells expressed and produced large amounts of both proteins. These results indicate that FN and LM are constantly present during the process of IEC-17 organoid maturation: we can hypothesize that the two proteins act as mediators of cell to cell and cell to substrate adhesion interactions and, probably, have an active regulatory role in the process of intestinal epithelial cell differentiation.
Scarpa
S
S
Department of Experimental Medicine, University of Rome La Sapienza.
D'Orazi
G
G
Modesti
A
A
Valia
S
S
Zucco
F
F
Stammati
A
A
D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism; D005353:Fibronectins / Q000032:analysis* / Q000096:biosynthesis; D005455:Fluorescent Antibody Technique; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000166:cytology; D007797:Laminin / Q000032:analysis* / Q000096:biosynthesis; D008854:Microscopy, Electron; D011233:Precipitin Tests; D051381:Rats
D005353:Fibronectins; D007797:Laminin