title: Stable analogues of prostacyclin and thromboxane A2 display contradictory influences on atherosclerotic properties of cells cultured from human aorta. The effect of calcium antagonists.
abstract: We examined the influence of stable prostacyclin analogues (carbacyclin) and thromboxane A2 (U46619) on atherosclerotic properties of cells: [3H]thymidine incorporation and intracellular cholesterol content. A primary culture of human aortic subendothelial cells derived from atherosclerotic plaques was used. Carbacyclin exerted a direct anti-atherosclerotic effect, significantly reducing atherosclerotic manifestations of cells, while agent U46619 stimulated proliferation and cholesterol accumulation, i.e. demonstrated atherogenic potency in culture. Calcium antagonists (verapamil and diltiazem) markedly enhanced anti-atherosclerotic properties of carbacyclin and restricted the atherogenicity of U46619.
authors.affiliation: Department of Pharmacology, Yerevan Medical Institute, Armenian SSR, U.S.S.R.
authors.forename: S E
authors.initials: SE
authors.lastname: Akopov
authors.forename: A N
authors.initials: AN
authors.lastname: Orekhov
authors.forename: V V
authors.initials: VV
authors.lastname: Tertov
authors.forename: K A
authors.initials: KA
authors.lastname: Khashimov
authors.forename: E S
authors.initials: ES
authors.lastname: Gabrielyan
authors.forename: V N
authors.initials: VN
authors.lastname: Smirnov
mesh_terms: D019796:15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid; D001011:Aorta / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D001161:Arteriosclerosis / Q000378:metabolism* / Q000473:pathology; D002478:Cells, Cultured; D004261:DNA Replication / Q000187:drug effects; D004110:Diltiazem / Q000494:pharmacology*; D011464:Epoprostenol / Q000494:pharmacology* / Q000031:analogs & derivatives; D006801:Humans; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D011450:Prostaglandin Endoperoxides, Synthetic / Q000494:pharmacology*; D011465:Prostaglandins, Synthetic / Q000494:pharmacology*; D014700:Verapamil / Q000494:pharmacology*
chemical_list: D011450:Prostaglandin Endoperoxides, Synthetic; D011465:Prostaglandins, Synthetic; C026678:carboprostacyclin; D019796:15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid; D014700:Verapamil; D011464:Epoprostenol; D004110:Diltiazem