Monoclonals and allergy. A review, with experimental contribution.
The role of monoclonal antibodies in allergy has been explored. First the status of art of monoclonal research in general is reviewed, by outlining a monoclonals identikit and the relevant technologies employed for their development. The attention is then focused on the present impact of monoclonals in the allergological field, first considering a general outline, and then the important steps of standardization of monoclonal antibodies. A comprehensive hint is made concerning the monitoring of immunotherapy, with future extrapolations on developing anti-idiotype vaccines, of which same examples can already be found in the infectious field, thus leaving the way open in allergy as well. A second section deals with the experimental contribution of the Authors, with the description of the preparation of the allergenic extracts of D.F., with details of the relevant steps (rabbit immunization; extract characterization; techniques used for monoclonal screening and characterization). The results obtained are discussed in relation to the techniques employed, weighing the reciprocal advantages and drawbacks.
Albertini
A
A
Istituto di Chimica e Propedeutica Biochimica, Università degli Studi di Brescia.
Antoniazzi
E
E
Archetti
S
S
Bugari
G
G
Cabula
R
R
Ghezzi
E
E
Ghielmi
S
S
Sacchi
G
G
Tassi
G C
GC
Tombesi
S
S
D000818:Animals; D000911:Antibodies, Monoclonal; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology / Q000628:therapy*; D007116:Immunization, Passive; D007158:Immunologic Techniques
D000911:Antibodies, Monoclonal