title: The kinetics of SHV-2 plasmid-mediated beta-lactamase compared to those of the parent enzyme from which it is derived.
abstract: Kinetic constants were determined for two closely related plasmid-mediated beta-lactamases: SHV-2 and PIT-2 (also known as SHV-1). These enzymes were synthesized constitutively. They were highly sensitive to the action of the inhibitors clavulanic acid and sulbactam, and they lacked activity against cephamycins and also imipenem. Both enzymes were significantly active against penicillins and first-generation cephalosporins, and the main difference concerned the third-generation cephalosporins: SHV-2 was highly active against these compounds whereas PIT-2 was not.
authors.affiliation: National Museum of Natural History, UA 401 CNRS, Paris, France.
authors.forename: R
authors.initials: R
authors.lastname: Labia
authors.forename: A
authors.initials: A
authors.lastname: Morand
authors.forename: K
authors.initials: K
authors.lastname: Tiwari
authors.forename: J S
authors.initials: JS
authors.lastname: Pitton
authors.forename: D
authors.initials: D
authors.lastname: Sirot
authors.forename: J
authors.initials: J
authors.lastname: Sirot
authors.forename: H
authors.initials: H
authors.lastname: Ben Yaghlane
authors.forename: A
authors.initials: A
authors.lastname: Boujenah
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002511:Cephalosporins / Q000494:pharmacology; D002969:Clavulanic Acids / Q000494:pharmacology; D004926:Escherichia coli / Q000201:enzymology*; D007700:Kinetics; D010957:Plasmids; D013407:Sulbactam / Q000494:pharmacology; D001618:beta-Lactamases / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D002511:Cephalosporins; D002969:Clavulanic Acids; D001618:beta-Lactamases; D013407:Sulbactam