A sensitive, rapid, chemiluminescence-based method for the determination of diamines and polyamines.
A new sensitive and rapid chemiluminescence-based method for the determination of diamines and polyamines is described. Phosphocellulose paper strips are used for the removal of neutral or negatively charged molecules from polyamine-containing fluid. The procedure is based on the determination of hydrogen peroxide, produced during the oxidation of polyamines, by a fairly specific serum amine oxidase. A plant diamine oxidase is used for the assay of diamines. This method permits the determination of diamines and polyamines in a range of 10 to 100 pmol and may be used for the assay of urinary polyamines.
Bachrach
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D006631:Amine Oxidase (Copper-Containing); D000818:Animals; D002417:Cattle; D002855:Chromatography, Thin Layer; D003959:Diamines / Q000032:analysis*; D007700:Kinetics; D008163:Luminescent Measurements; D000587:Oxidoreductases Acting on CH-NH Group Donors; D011073:Polyamines / Q000032:analysis* / Q000652:urine; D012015:Reference Standards
D003959:Diamines; D011073:Polyamines; D006631:Amine Oxidase (Copper-Containing); D000587:Oxidoreductases Acting on CH-NH Group Donors