title: A perfusion chamber for high-resolution light microscopy of cultured cells.
abstract: A perfusion chamber suitable for general microscopic work on upright and inverted microscopes with cultured cells is described. The device features a minimal internal volume and large field of view within small outer dimensions. In particular the chamber body is very flat to accommodate large-aperture condenser and objective lenses. It is constructed of innocuous, sterilizable materials, incorporates permanent seals for the windows, is robust and simple to use.
authors.affiliation: Abteilung Spektroskopie, Max-Planck-Institut für Biophysikalische Chemie, Göttingen, F.R.G.
authors.forename: S A
authors.initials: SA
authors.lastname: Finch
authors.forename: A
authors.initials: A
authors.lastname: Stier
mesh_terms: D002478:Cells, Cultured; D003584:Cytological Techniques / Q000295:instrumentation*; D007700:Kinetics; D008853:Microscopy / Q000379:methods*