Enhancement of leukocyte cytotoxicity after exercise-induced asthma.
We have previously shown that there were elevations of neutrophil chemotactic activity (NCA) and increases in the percentages of neutrophil and monocyte complement rosettes after exercise-induced asthma (EIA). These observations suggested that leukocyte activation may occur after EIA, possibly as a result of the release of mast-cell-associated mediators. In the present study, we have attempted to establish whether neutrophils and monocytes are functionally altered after EIA as assessed by changes in their cytotoxic capacity. Cytotoxicity was assessed by a direct visual killing assay using opsonized (complement-coated) schistosomula of Schistosoma mansoni as target organisms. Neutrophils and mononuclear cells obtained from 8 patients after exercise-induced asthma (EIA+ve) had increased cytotoxicity for opsonized schistosomula for as long as 60 min after exercise. These changes were preceded by elevations in the concentrations of serum high molecular weight NCA (which were maximal at 10 min after exercise). In asthmatic patients who did not develop exercise-induced asthma (EIA-ve), no significant increases in neutrophil or mononuclear cell killing of schistosomula, or serum NCA concentrations, were observed. There was a highly significant correlation (p less than 0.001) between the reduction in FEV1 and the increases in neutrophil cytotoxicity. In 5 EIA+ve patients, administration of disodium cromoglycate (cromolyn) prior to the exercise task inhibited both the enhancement in neutrophil and mononuclear cell cytotoxicity, as well as the elevations in circulating NCA and the reductions in FEV1.(ABSTRACT TRUNCATED AT 250 WORDS)
Moqbel
R
R
Durham
S R
SR
Shaw
R J
RJ
Walsh
G M
GM
MacDonald
A J
AJ
Mackay
J A
JA
Carroll
M P
MP
Kay
A B
AB
D000293:Adolescent; D000328:Adult; D001249:Asthma / Q000276:immunology*; D001250:Asthma, Exercise-Induced / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D002630:Chemotactic Factors / Q000378:metabolism; D004205:Cromolyn Sodium / Q000494:pharmacology; D003602:Cytotoxicity, Immunologic / Q000187:drug effects*; D005260:Female; D005541:Forced Expiratory Volume; D006801:Humans; D016209:Interleukin-8; D007962:Leukocytes / Q000276:immunology*; D010587:Phagocytosis; D012550:Schistosoma mansoni / Q000276:immunology
D002630:Chemotactic Factors; D016209:Interleukin-8; D004205:Cromolyn Sodium