High-performance liquid chromatography of porphyrins.
Techniques for the analysis of porphyrins in the biomedical fields are reviewed. The emphasis is on high-performance liquid chromatography and its aspplications in: (1) the quantitative analysis of porphyrins in blood, urine and faeces; (2) qualitative porphyrin profiles in normal subjects and in the porphyrias; (3) assay of haem biosynthetic enzyme activities and (4) resolution of type isomers of porphyrins and porphyrinogens. Detection systems, quantitation methods, peak identification and sample preparation procedures are discussed.
Lim
C K
CK
Division of Clinical Cell Biology, MRC Clinical Research Centre, Harrow, Middlesex, U.K.
Li
F M
FM
Peters
T J
TJ
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D011166:Porphyrins / Q000032:analysis*
D011166:Porphyrins