title: Peptide immunocytochemistry in afferent neurons from lower gut in rats.
abstract: Several peptides were detected in primary sensory neurons located in nodose and dorsal root ganglia and projecting from rat cecum and rectosigmoid, through a combination of retrograde staining by the fluorescent tracer DY-2HCl and of the immunofluorescent procedure of Coons. The three larger cell populations thus identified stored immunoreactive components respectively similar to calcitonin gene-related peptide (CGRP), substance P (SP), and a peptide related to peptide histidine methionine (PHM). The later immunoreactivity consisted of a single molecular form with an apparent molecular weight smaller than PHM itself. Fewer cells contained components immunologically similar to somatostatin 14 (ST14), to the 1-14 N-terminal sequence of somatostatin 28 (1-14 S28), and to neuropeptide Y (NPY). Neonatal treatment with capsaicin resulted in a drastic reduction of immunoreactivity for SP, PHM, ST14, 1-14 S28, and in a partial reduction of CGRP-like positive perikarya. These results demonstrate that several peptides are potentially involved in the sensory innervation of the lower gut in rat.
authors.affiliation: INSERM U45, Hôpital E. Herriot, Lyon, France.
authors.forename: S
authors.initials: S
authors.lastname: Chery-Croze
authors.forename: A
authors.initials: A
authors.lastname: Bosshard
authors.forename: H
authors.initials: H
authors.lastname: Martin
authors.forename: J C
authors.initials: JC
authors.lastname: Cuber
authors.forename: Y
authors.initials: Y
authors.lastname: Charnay
authors.forename: J A
authors.initials: JA
authors.lastname: Chayvialle
mesh_terms: D000344:Afferent Pathways / Q000166:cytology*; D000818:Animals; D002432:Cecum / Q000294:innervation*; D004386:Duodenum / Q000294:innervation*; D005260:Female; D005455:Fluorescent Antibody Technique; D005727:Ganglia, Spinal / Q000166:cytology; D009474:Neurons / Q000166:cytology*; D009479:Neuropeptides / Q000032:analysis*; D009620:Nodose Ganglion / Q000166:cytology; D051381:Rats; D011919:Rats, Inbred Strains; D013116:Spinal Cord / Q000032:analysis / Q000166:cytology
chemical_list: D009479:Neuropeptides