Trypanosoma cruzi: studies on the interactions of lectins with glycoconjugates of different zymodemes.
Detergent extracts were made of eight strains of Trypanosoma cruzi which were representative of the principal zymodemes. The extracts were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis and the glycoproteins were reacted with 21 different 125I-labeled lectins and autoradiographed. The staining patterns with particular lectins varied considerably between strains. Concanavalin A stained up to 17 distinct bands in some strains. Other lectins such as peanut lectin only stained two bands in zymodeme 1 strains and none in the other zymodemes. The reaction of N-acetylgalactosamine-specific lectins with some bands indicated the presence of this sugar and this was confirmed by analysis of the extracts. The lectin staining patterns provided an insight into the glycoprotein composition of the bands and should indicate whether combinations of lectins can be used in affinity chromatography systems to purify the glycoproteins.
Stevens
A F
AF
Department of Biochemistry, Charing Cross and Westminster Medical School, London, U.K.
Miles
M A
MA
Allen
A K
AK
D000818:Animals; D001345:Autoradiography; D002241:Carbohydrates / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D006001:Glycoconjugates / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism; D037102:Lectins / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000032:analysis / Q000302:isolation & purification; D014349:Trypanosoma cruzi / Q000032:analysis* / Q000145:classification
D002241:Carbohydrates; D006001:Glycoconjugates; D037102:Lectins; D008562:Membrane Glycoproteins