Evidence that Lyb-5 is a differentiation antigen in normal and xid mice.
These studies addressed the question of whether the Lyb-5 determinant on mouse B cells might represent a marker for a unique lineage of B cells as has been thought, or rather, a differentiation antigen. Previous studies have addressed this question by treating splenic B cells with anti-Lyb-5 + complement and then culturing them with various antigens. In this study, we first immunized in vivo or in vitro and then determined susceptibility to anti-Lyb-5. These studies demonstrate that a substantial proportion of antibody-forming cells produced in response to immunization with sheep red blood cells, trinitrophenyl (TNP)-keyhole limpet hemocyanin, TNP-Brucella abortus, TNP-lipopolysaccharide, as well as TNP-Ficoll, are eliminated by anti-Lyb-5 + complement treatment at the end of culture. Some generation of Lyb-5+ antibody-forming cells occurred in the last 24 hr of culture. These studies suggest that the Lyb-5 marker represents a differentiation antigen on relatively mature B cells.
Smith
H R
HR
Yaffe
L J
LJ
Kastner
D L
DL
Steinberg
A D
AD
D000818:Animals; D000944:Antigens, Differentiation, B-Lymphocyte; D000950:Antigens, Ly / Q000032:analysis / Q000276:immunology*; D000954:Antigens, Surface / Q000032:analysis / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D002003:Brucella abortus / Q000276:immunology; D003165:Complement System Proteins / Q000502:physiology; D003602:Cytotoxicity, Immunologic; D005260:Female; D006151:Guanosine / Q000031:analogs & derivatives / Q000494:pharmacology; D006462:Hemolytic Plaque Technique; D007074:Immunoglobulin G / Q000096:biosynthesis; D007518:Isoantibodies / Q000502:physiology; D008070:Lipopolysaccharides / Q000276:immunology; D008213:Lymphocyte Activation / Q000187:drug effects; D008297:Male; D051379:Mice; D008811:Mice, Inbred DBA; D008814:Mice, Inbred NZB; D013154:Spleen / Q000166:cytology; D013997:Time Factors; D014301:Trinitrobenzenes / Q000276:immunology; D014960:X Chromosome / Q000276:immunology
D000944:Antigens, Differentiation, B-Lymphocyte; D000950:Antigens, Ly; D000954:Antigens, Surface; D007074:Immunoglobulin G; D007518:Isoantibodies; D008070:Lipopolysaccharides; D014301:Trinitrobenzenes; D006151:Guanosine; C035169:8-bromoguanosine; D003165:Complement System Proteins