Use of the collagen I-deficient Mov13 mouse mutant to analyse epithelial-mesenchymal tissue interaction.
The collagen I-deficient mouse mutant (Mov13 - an embryonic recessive lethal) was used to investigate the function of this major constituent of the extracellular matrix (ECM) in organ development. All epithelial-mesenchymal organs tested as explants (lung, kidney, pancreas, salivary glands, skin) developed normally and, in particular, showed typical branching morphogenesis in the absence of collagen I. It is concluded that the ECM of these organs can organize for proper developmental function in the absence of the major interstitial collagen, but a possible morphogenetic function of other fibrillar collagens (types III and V) cannot be excluded. The only insufficiencies in the mutant were seen in the cornea where deposition and organization of the collagenous stroma was highly inadequate; but even there, development and migration of cells proceeded normally. In summary, the results indicate that 'cellular' development in epithelial-mesenchymal organs (including growth, morphogenesis, and differentiation) does not depend on collagen I.
Kratochwil
K
K
Institut für Molekularbiologie der Osterreichischen Akademie der Wissenschaften, Salzburg, Austria.
D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism*; D005314:Embryonic and Fetal Development; D004847:Epithelial Cells; D008648:Mesoderm / Q000166:cytology* / Q000378:metabolism; D051379:Mice
D003094:Collagen