Use of site-directed mutagenesis to probe the role of Cys149 in the formation of charge-transfer transition in glyceraldehyde-3-phosphate dehydrogenase.
Oligonucleotide-directed mutagenesis was employed to produce mutants of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) of Escherichia coli and Bacillus stearothermophilus. Three different mutants proteins--His176----Asn, Cys149----Ser, Cys149----Gly--were isolated from one or both of the enzymes. The study of the properties of these mutants has shown that Cys149 is clearly responsible for the information of a charge-transfer transition, named the Racker band, observed during the NAD+ binding to apoGAPDH. This result excludes a similarity between the Racker band and the charge-transfer transition observed following the alkylation of GAPDH by 3-chloroacetyl pyridine-adenine dinucleotide.
Mougin
A
A
Laboratoire d'Enzymologie et de Génie Génétique, UA CNRS 457, Faculté des Sciences, Vandoeuvre-les-Nancy, France.
Corbier
C
C
Soukri
A
A
Wonacott
A
A
Branlant
C
C
Branlant
G
G
D001665:Binding Sites; D003545:Cysteine; D004926:Escherichia coli / Q000201:enzymology; D001411:Geobacillus stearothermophilus / Q000201:enzymology; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics* / Q000378:metabolism; D009154:Mutation
D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D003545:Cysteine