In vivo antiaggregatory effect of BM 13.177 on the spontaneous platelet aggregation in anaesthetized beagle dogs.
The arterial blood of anaesthetized heparin-treated beagle dogs was directed through a 30/um diameter pore size screen by a roller pump at a constant rate. As a result, the pressure proximal to the filter continuously increased. The filtration pressure stabilizing concentration of prostacyclin (infused proximal to the filter) was determined. BM 13.177 -similar to PGI2- was able to slow down and stop the increase of filtration pressure (final conc.: 1-10/uM). In addition it could reverse the filter occlusion process. The lowest concentration of BM 13.177 (0.1-1/uM) did not affect significantly the filter occlusion rate, but it markedly enhanced the antiaggregatory effect of PGI2 when these drugs were administered simultaneously. In summary, we can conclude that 1) generation of thromboxane and endoperoxide plays a key role in the mechanism of the spontaneous platelet aggregation on the filter; 2) BM 13.177 significantly potentiates the antiaggregatory effect of PGI2 in vivo.
Hermán
F
F
Department of Pharmacodynamics, Semmelweis University of Medicine, Budapest, Hungary.
Malomvölgyi
B
B
Hadházy
P
P
Magyar
K
K
D000818:Animals; D004285:Dogs; D011464:Epoprostenol / Q000494:pharmacology; D066298:In Vitro Techniques; D007262:Infusions, Intravenous; D010975:Platelet Aggregation Inhibitors / Q000008:administration & dosage / Q000494:pharmacology*; D013449:Sulfonamides / Q000494:pharmacology*
D010975:Platelet Aggregation Inhibitors; D013449:Sulfonamides; C040624:sulotroban; D011464:Epoprostenol