Inhibition of Pseudomonas aeruginosa from cystic fibrosis by selective media.
Pseudomonas Isolation Agar (selective agent, Irgasan, 25 mg/1) and Pseudomonas Selective Agar (selective agents, cetrimide 200mg/1 and nalidixic acid 15 mg/1) inhibited some strains of P aeruginosa from cystic fibrosis sputum but did not inhibit isolates from other sources. Of 200 cystic fibrosis isolates, 22 were inhibited by 16 mg/1 Irgasan, 45 by 8 mg/1 nalidixic acid, and 15 by 128 mg/1 cetrimide. We recommend that cystic fibrosis sputum should be cultured on selective and non-selective media to maximise the isolation of P aeruginosa.
Fonseca
K
K
MacDougall
J
J
Pitt
T L
TL
D002224:Carbanilides / Q000494:pharmacology; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000494:pharmacology; D003470:Culture Media; D003550:Cystic Fibrosis / Q000382:microbiology*; D006801:Humans; D009268:Nalidixic Acid / Q000494:pharmacology; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000302:isolation & purification*; D013183:Sputum / Q000382:microbiology
D002224:Carbanilides; D002593:Cetrimonium Compounds; D003470:Culture Media; D009268:Nalidixic Acid; C005055:cloflucarban; D000077286:Cetrimonium