title: Trypanosoma congolense: interactions between trypanosomes expressing different metacyclic variable antigen types in vitro and in vivo.
abstract: In Trypanosome congolense, the surface antigens expressed by cultured metacyclic forms are a limited and serodeme-specific subset of variable antigen types (VATs). Experiments were carried out in mammalian-form (MF) cultures, comprising dividing trypanosomes which express metacyclic VATs (M-VATs) maintained in vitro with a mammalian cell feeder layer, or in mice following infection with cultured metacyclic populations. Selective neutralization experiments were performed by incubating populations of trypanosomes with one or a combination of McAbs to remove their respective M-VATs. Deletion of one M-VAT from the population had no effect on expression and growth of other unrelated M-VATs. Deleted M-VATs were detected in very small proportions of the total population 2-7 days following neutralization. Subsequent incubation of neutralized cultures resulted in growth of deleted M-VATs which, in most cases, reached pre-neutralization levels. It appears, therefore, that in conditions where expression of M-VATs on dividing trypanosomes occurs, removal of individual M-VATs results in their re-expression within the population.
authors.affiliation: Centre for Tropical Veterinary Medicine, University of Edinburgh, Roslin, Midlothian, Scotland.
authors.forename: C J
authors.initials: CJ
authors.lastname: Prain
authors.forename: C A
authors.initials: CA
authors.lastname: Ross
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000913:Antibodies, Protozoan / Q000276:immunology; D000953:Antigens, Protozoan / Q000032:analysis*; D000954:Antigens, Surface / Q000032:analysis; D002460:Cell Line; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D051379:Mice; D009500:Neutralization Tests; D014348:Trypanosoma congolense / Q000276:immunology*; D014643:Variant Surface Glycoproteins, Trypanosoma / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D000954:Antigens, Surface; D014643:Variant Surface Glycoproteins, Trypanosoma