title: Characterization of protein-linked oligosaccharides in trypanosomatid flagellates.
abstract: Protein-linked, endo-beta-N-acetylglucosaminidase H-sensitive oligosaccharides were isolated from several trypanosomatids incubated with [U-14C]glucose. Structural analysis of the compounds revealed that Man9GlcNAc2 was the oligosaccharide transferred from dolichol-P-P derivatives to proteins in Trypanosoma dionisii, Trypanosoma conorhini, Leptomonas samueli and Herpetomonas samuelpessoai and Man6GlcNAc2 in Blastocrithidia culicis and Leishmania adleri. In all cases, transiently glucosylated compounds were detected: Glc1Man7-9GlcNAc2 in T. dionisii, T. conorhini, L. samueli; Glc1Man9GlcNAc2 in H. samuelpessoai, Glc1Man6GlcNAc2 in B. culicis and Glc1Man6GlcNAc2 and Glc1Man5GlcNAc2 in L. adleri. The mechanism of protein glycosylation in T. dionisii and T. conorhini appeared to be similar to that described before for Trypanosoma cruzi epimastigotes, although some differences were found between the structures of the main isomers of Man7GlcNAc2 and Man8GlcNAc2 present in T. conorhini and T. cruzi. Differences between the mechanisms of glycosylation occurring in Leishmania mexicana and L. adleri were also found: Man6GlcNAc2 in the latter microorganism was demannosylated to Man5GlcNAc2, a step not detected in the former parasite. A novel substituent in N-linked high mannose-type oligosaccharides was found in L. samueli and H. samuelpessoai: galactose in the furanose configuration. In the latter trypanosomatid, Man9GlcNAc2 was demannosylated only to Man8GlcNAc2, whereas in all other parasites in which the same oligosaccharide was transferred to proteins, Man5-7GlcNAc2 were also detected.
authors.forename: D H
authors.initials: DH
authors.lastname: Mendelzon
authors.forename: J O
authors.initials: JO
authors.lastname: Previato
authors.forename: A J
authors.initials: AJ
authors.lastname: Parodi
mesh_terms: D000818:Animals; D004288:Dolichol Phosphates / Q000378:metabolism; D006023:Glycoproteins / Q000032:analysis* / Q000096:biosynthesis; D009844:Oligosaccharides / Q000032:analysis* / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D014351:Trypanosomatina / Q000032:analysis* / Q000378:metabolism
chemical_list: D004288:Dolichol Phosphates; D006023:Glycoproteins; D009844:Oligosaccharides