Room temperature, microtray chromogenic assay of factor VIII:C.
Commercial reagents of non-human origin were used to provide a room temperature, microtray chromogenic assay for factor VIII. The assay allows determination of factor VIII in the range 15-150% of normal plasma levels with a coefficient of variation of 3-4%. Haemophilic plasmas and factor VIII concentrates may also be assayed by varying the pre-dilution step. A good correlation was demonstrated with results obtained using an established one-stage clotting assay (r = 0.9985).
Prowse
C
C
Hornsey
V
V
McKay
G
G
Waterston
Y
Y
D005169:Factor VIII / Q000032:analysis*; D005612:Freeze Drying; D006467:Hemophilia A / Q000097:blood; D006801:Humans; D007202:Indicators and Reagents; D008832:Microchemistry; D012016:Reference Values; D013053:Spectrophotometry / Q000379:methods; D013696:Temperature
D007202:Indicators and Reagents; D005169:Factor VIII