Suppression of synthesis of tropomyosin isoform 2 in metastatic v-Ha-ras-transformed NIH3T3 cells.
We have reported that the synthesis of tropomyosin isoform 2 (TM2) is significantly suppressed in high-metastatic Lewis lung carcinoma cells compared with that in low-metastatic cells. In order to examine whether the change is also observed in other high-metastatic tumor cells, we compared the pattern of expressions of TM isoforms between NIH3T3 cells and v-Ha-ras-transformed NIH3T3 (pH1-3) cells, the latter of which was highly metastatic when injected into BALB/c nude mice. The results showed that the expression of TM2 was less in pH1-3 cells than in NIH3T3 cells, suggesting that the suppression of TM2 synthesis is involved in the expression of metastatic phenotype.
Takenaga
K
K
Division of Chemotherapy, Chiba Cancer Center Research Institute, Japan.
Nakamura
Y
Y
Sakiyama
S
S
D000818:Animals; D015152:Blotting, Northern; D002461:Cell Line, Transformed; D002471:Cell Transformation, Neoplastic / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005786:Gene Expression Regulation; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D009693:Nucleic Acid Hybridization; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D016283:Proto-Oncogene Proteins p21(ras); D015347:RNA Probes; D012333:RNA, Messenger / Q000378:metabolism; D014335:Tropomyosin / Q000096:biosynthesis* / Q000235:genetics; D014407:Tumor Cells, Cultured
D011518:Proto-Oncogene Proteins; D015347:RNA Probes; D012333:RNA, Messenger; D014335:Tropomyosin; D016283:Proto-Oncogene Proteins p21(ras)