Phospholipid and energy metabolism of cancer cells monitored by 31P magnetic resonance spectroscopy: possible clinical significance.
31P magnetic resonance spectroscopy (MRS) has been established as an excellent noninvasive procedure for studying metabolism in cancer cells. For a full understanding of the results of in vivo MRS studies of metabolism, it is necessary to be able to conduct controlled experiments on the same intact functioning cells in a perfusion apparatus. The specific techniques used for this purpose are described, and the applications to cancer cell bioenergetics and phospholipid metabolism in drug-resistant and drug-sensitive breast cancer cells are illustrated. Spectra of the same cell line grown in a "model tumor" in natural basement membrane gel and in nude mice in vivo are compared. The results indicate a potential for the use of 31P MRS in a clinical setting for monitoring therapy and for diagnosis.
Cohen
J S
JS
Medicine Branch, National Cancer Institute, Bethesda, MD 20892.
D000230:Adenocarcinoma / Q000378:metabolism*; D000818:Animals; D001943:Breast Neoplasms / Q000378:metabolism*; D002460:Cell Line; D004734:Energy Metabolism; D005260:Female; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008325:Mammary Neoplasms, Experimental / Q000378:metabolism; D051379:Mice; D008819:Mice, Nude; D010743:Phospholipids / Q000378:metabolism*; D010759:Phosphorus Isotopes
D010743:Phospholipids; D010759:Phosphorus Isotopes