title: Sensitivity and specificity of latex agglutination tests used to identify Streptococcus agalactiae and Staphylococcus aureus isolated from bulk tank milk.
abstract: Comparisons were made among rapid latex agglutination tests and conventional biochemical tests used to identify Streptococcus agalactiae and Staphylococcus aureus. Ninety-eight streptococci and 149 staphylococci isolated from bulk tank milk were tested. Sensitivity and specificity for the latex agglutination test used for identification of Str agalactiae were 97.6 and 98.2%, respectively. Sensitivity and specificity for the latex agglutination test used for identification of S aureus were 90.2 and 67.5%, respectively. Of 25 staphylococci considered false-positive by the latex agglutination test, 14 (56%) were considered tube coagulase-positive. Fifteen staphylococci considered false-positive by latex agglutination test had biotypes representative of S hyicus of S xylosus.
authors.affiliation: Department of Dairy Science, Ohio Agricultural Research and Development Center, Ohio State University, Wooster 44691.
authors.forename: J S
authors.initials: JS
authors.lastname: Hogan
authors.forename: K L
authors.initials: KL
authors.lastname: Smith
authors.forename: D A
authors.initials: DA
authors.lastname: Todhunter
authors.forename: P S
authors.initials: PS
authors.lastname: Schoenberger
mesh_terms: D000818:Animals; D002417:Cattle; D005260:Female; D007841:Latex Fixation Tests; D008414:Mastitis, Bovine / Q000175:diagnosis / Q000382:microbiology; D008892:Milk / Q000382:microbiology*; D013203:Staphylococcal Infections / Q000175:diagnosis / Q000662:veterinary; D013211:Staphylococcus aureus / Q000032:analysis* / Q000302:isolation & purification; D013290:Streptococcal Infections / Q000175:diagnosis / Q000662:veterinary; D013292:Streptococcus agalactiae / Q000032:analysis* / Q000302:isolation & purification