Capacity of alloreactive human T clones to produce factor(s) inducing proliferation of the IL3-dependent DA-1 murine cell line. I. Evidence that this production is under IL2 control.
Several T-lymphocyte clones obtained from rejected human kidney allografts and maintained for several months in recombinant IL2 and antigen-supplemented cultures were studied for their capacity to produce lymphokines in vitro. Six clones produced a factor able to increase 3HTdR uptake of the IL3-dependent DA-1 murine cell line. All were T4+, T3+ and T11+ and fitted with a probability of monoclonality above 97%. The factor, designated as human-interleukin-DA (HILDA), was not produced when autologous EBV-transformed B cells were added in the culture in the absence of exogenous IL2. Addition of pure recombinant IL2 along with donor EBV-transformed cell lines resulted in a sharp increase in HILDA yield, whereas a low amount of this factor was also produced with the autologous EBV B lymphocyte in the presence of exogenous IL2. Kinetics studies show that HILDA was detectable as early as 24 to 48 h, peaked at day 3 and plateaued until day 5. The antigen-exogenous IL2-driven pathway of HILDA production by clones was bypassed by use of either PMA or calcium ionophore (CaI) alone or associated in the culture. Both compounds induced dose-related HILDA production (without antigen and/or exogenous IL2). No synergistic effect of PMA and CaI was noted, although an additional effect could be seen when a suboptimal dose of CaI was used.
Moreau
J F
JF
Bonneville
M
M
Peyrat
M A
MA
Jacques
Y
Y
Soulillou
J P
JP
D000818:Animals; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000032:analysis; D002460:Cell Line; D002462:Cell Membrane / Q000276:immunology; D002478:Cells, Cultured; D002999:Clone Cells; D003602:Cytotoxicity, Immunologic; D006084:Graft Rejection; D006133:Growth Substances / Q000276:immunology*; D006801:Humans; D007376:Interleukin-2 / Q000276:immunology; D007377:Interleukin-3; D016030:Kidney Transplantation; D008222:Lymphokines / Q000096:biosynthesis / Q000276:immunology*; D051379:Mice; D011994:Recombinant Proteins / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D014184:Transplantation, Homologous
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D006133:Growth Substances; D007376:Interleukin-2; D007377:Interleukin-3; D008222:Lymphokines; D011994:Recombinant Proteins