Increased rate of cholic acid formation from 3 alpha,7 alpha-dihydroxy-5 beta-cholestane in perfused livers from diabetic rats.
The formation of bile acids from 3 alpha,7 alpha-dihydroxy-5 beta-cholestane was comparatively investigated in the perfused livers from normal, streptozotocin-diabetic, and insulin-treated diabetic rats. During the infusion of the sterol substrate, cholic acid accounted for only 11% of the total biliary bile acids in the normal rats, while it increased drastically to 57% in the diabetic rats. This increased proportion of cholic acid tended to be normalized by treatment of the diabetic animals with insulin. These results suggest the possible acceleration of cholic acid formation, involving 12 alpha-hydroxylation of 3 alpha,7 alpha-dihydroxy-5 beta-cholestane or one of its metabolites in an insulin-deficient state in rats.
Kimura
K
K
Department of Biochemistry, Tottori University School of Medicine, Yonago, Japan.
Ogura
Y
Y
Ogura
M
M
D000818:Animals; D001646:Bile / Q000378:metabolism; D001647:Bile Acids and Salts / Q000378:metabolism; D002777:Cholestanols / Q000378:metabolism*; D019826:Cholic Acid; D002793:Cholic Acids / Q000096:biosynthesis*; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D007328:Insulin / Q000494:pharmacology; D008099:Liver / Q000378:metabolism*; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values
D001647:Bile Acids and Salts; D002777:Cholestanols; D002793:Cholic Acids; D007328:Insulin; C007426:dihydroxycoprostane; D019826:Cholic Acid