A rapid in vitro assay system using anti-bromodeoxyuridine for drug susceptibility of Plasmodium falciparum.
Drug effects were monitored by the measurement of bromodeoxyuridine (BrdU) incorporation into parasite deoxyribonucleic acid (DNA). Cells are pulse-labelled with BrdU and those which are synthesizing DNA incorporate BrdU into their DNA. Anti-BrdU is used to identify cells undergoing DNA synthesis at the time of the pulse. Concentration-effect curves were determined for chloroquine, pyrimethamine and quinine in culture for only 3 h in RPMI 1640 medium supplemented with BrdU. Different strains of Plasmodium falciparum could be distinguished when BrdU uptake was monitored by the ELISA. This assay offers a fast and accurate method of monitoring the effects of a variety of antimalarial drugs.
Doi
H
H
Department of Parasitology, Okayama University Medical School, Japan.
Ishii
A
A
Shimono
K
K
D000818:Animals; D001973:Bromodeoxyuridine / Q000378:metabolism; D002738:Chloroquine / Q000494:pharmacology*; D004351:Drug Resistance; D004797:Enzyme-Linked Immunosorbent Assay; D010963:Plasmodium falciparum / Q000187:drug effects* / Q000378:metabolism; D011739:Pyrimethamine / Q000494:pharmacology*; D011803:Quinine / Q000494:pharmacology*
D002738:Chloroquine; D011803:Quinine; D001973:Bromodeoxyuridine; D011739:Pyrimethamine