Constitutive and inducible expression of cloned human interferon-b1 gene in HeLa cells through an episomal eukaryotic vector.
An EcoRI fragment (1.83 Kb) of human DNA containing B1-interferon coding sequences was cloned in the episomal eukaryotic vector pBK-TK. The recombinant plasmid was transfected into thymidine kinase-deficient HeLa cells. Thymidine kinase-positive transformants were obtained and the cells were found to express B-interferon both constitutively and after induction with Newcastle Disease Virus or poly(rI)-poly(rC). The recombinant plasmid was present in stably transformed HeLa cells in an episomal state in hundreds of copies per cell.
Giannì
M
M
Istituto di Genetica Biochimica ed Evoluzionistica CNR, Pavia, Italy.
Gallina
A
A
Barbanti-Brodano
G
G
Milanesi
G
G
D015139:Blotting, Southern; D003001:Cloning, Molecular; D004274:DNA, Recombinant; D005786:Gene Expression Regulation; D006367:HeLa Cells / Q000378:metabolism; D006801:Humans; D007163:Immunosorbent Techniques; D007370:Interferon Type I / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D012333:RNA, Messenger / Q000096:biosynthesis; D011994:Recombinant Proteins; D013937:Thymidine Kinase / Q000235:genetics; D014158:Transcription, Genetic; D014162:Transfection; D014170:Transformation, Genetic
D004274:DNA, Recombinant; D007370:Interferon Type I; D012333:RNA, Messenger; D011994:Recombinant Proteins; D013937:Thymidine Kinase