Immunoaffinity isolation of a sialyl-Le(a) oligosaccharide from human milk.
A cancer-associated antigen, sialyl-Le(a) oligosaccharide, was isolated from human milk using a monoclonal antibody recognizing carbohydrate moieties of mucin-type glycoproteins. The structure was identified as: (Formula: see text) based on 500-MHz 1H-NMR spectroscopy. This oligosaccharide comprises 0.07% of sialyloligosaccharides in human milk. The NMR spectra of two fellow oligosaccharides, Le(a) oligosaccharide (or lacto-N-fucopentaose II) and LS-tetrasaccharide a, are also given.
Kitagawa
H
H
Department of Biological Chemistry, Faculty of Pharmaceutical Sciences, Kyoto University.
Nakada
H
H
Numata
Y
Y
Kurosaka
A
A
Fukui
S
S
Funakoshi
I
I
Kawasaki
T
T
Shimada
K
K
Inagaki
F
F
Yamashina
I
I
D000911:Antibodies, Monoclonal / Q000276:immunology; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000302:isolation & purification*; D002846:Chromatography, Affinity / Q000379:methods; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007163:Immunosorbent Techniques; D009682:Magnetic Resonance Spectroscopy; D008895:Milk, Human / Q000378:metabolism*; D009077:Mucins / Q000096:biosynthesis; D009844:Oligosaccharides / Q000302:isolation & purification*
D000911:Antibodies, Monoclonal; D015295:Antigens, Tumor-Associated, Carbohydrate; D009077:Mucins; D009844:Oligosaccharides; C058981:sialyl-Le(a) oligosaccharide