[Microbiological determination of DNA levels in blood leukocytes].
A procedure is developed for estimation of DNA microquantities in biological samples, which involved acid hydrolysis of DNA and the following microbiological estimation of its concentration. A sensitivity of the procedure was as little as 0.25 micrograms DNA per ml. At the DNA concentration 0.25-10 micrograms/ml growth of the indicator strain of bacteria exhibited the linear shape. As shown by means of the procedure developed one rat leukocyte contained 6.3 +/- 0.6 X 10(-12) g of DNA.
Blokh
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Paskevich
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IF
D000818:Animals; D001681:Biological Assay; D004247:DNA / Q000032:analysis*; D006868:Hydrolysis; D007779:Lactobacillus acidophilus / Q000254:growth & development; D007962:Leukocytes / Q000032:analysis*; D008297:Male; D008828:Microbiological Techniques; D051381:Rats; D011919:Rats, Inbred Strains
D004247:DNA
Mikrobiologicheskoe opredelenie kontsentratsii DNK v leĭkotsitakh krovi.