[Pathogenicity of Spiroplasma sabaudiense (mollicute) for the cells (c6/36) of Aedes albopictus (Insecta: Diptera) in vitro].
An initial stock of Spiroplasma sabaudiense had been maintained in cell-free medium for 2-3 years. Subsequent passages in Leibovitz's medium L-15 in the presence of Aedes albopictus (C6/36) cells dramatically increased the pathogenicity of S. sabaudiense towards these cells. Cytopathogenicity included production of syncytia, an increase in the number of mitochondria and alteration of their morphology, vacuolisation and a reduction in the rate of growth. Cell lysis was associated with cytadsorption by spiroplasmas and was apparent following the third passage. At six days post-inoculation during the fourth and fifth passages, cell cultures had been totally destroyed by S. sabaudiense. The intra-cellular stage of S. sabaudiense is described and the literature pertaining to the pathogenicity of spiroplasmas for insects is reviewed.
Humphery-Smith
I
I
Département de Microbiologie et Santé publique, Faculté de Médecine, Brest, France.
Grulet
O
O
Le Lay
G
G
Chastel
C
C
D000330:Aedes / Q000382:microbiology* / Q000648:ultrastructure; D000818:Animals; D002460:Cell Line; D005455:Fluorescent Antibody Technique; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D013149:Spiroplasma / Q000254:growth & development*
Pathogénicité de Spiroplasma sabaudiense (mollicute) pour les cellules (C6/36) d'Aedes albopictus (Insecta: Diptera) in vitro.