Construction of a recombinant expression plasmid encoding a staphylococcal protein A-ricin A fusion protein.
A recombinant plasmid has been constructed containing the coding regions for a functional fragment of staphylococcal protein A and the entire ricin A chain in tandem in the same reading frame. The recombinant gene has been expressed in Escherichia coli cells to produce a protein with both staphylococcal protein A activity and ricin A chain activity. Such a fusion protein could be used for producing immunotoxins.
Kim
J H
JH
Department of Biochemistry, University of Kansas, Lawrence 66045.
Weaver
R F
RF
D002460:Cell Line; D002872:Chromosome Deletion; D003001:Cloning, Molecular / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics; D005455:Fluorescent Antibody Technique; D005796:Genes; D006801:Humans; D000922:Immunotoxins; D010957:Plasmids; D011993:Recombinant Fusion Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics*; D012276:Ricin / Q000032:analysis / Q000235:genetics*; D013205:Staphylococcal Protein A / Q000032:analysis / Q000235:genetics*
D000922:Immunotoxins; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D013205:Staphylococcal Protein A; C057975:staphylococcal protein A-ricin A fusion protein, recombinant; D012276:Ricin