title: Activation of intracellular serine proteinase in Bacillus subtilis cells during sporulation.
abstract: Cells of Bacillus subtilis 168 (trpC2) growing and sporulating in a single chemically defined medium carried out intracellular protein degradation and increased their levels of intracellular serine protease-1 in a manner very similar to what had previously been reported for cells sporulating in nutrient broth. The results were interpreted to mean that these processes are intrinsic to sporulation rather than medium dependent. To determine the cause of these increases in specific activity of proteinases, we purified the protease, prepared rabbit immunoglobulins directed against it, and monitored changes in protease antigen levels by performing rocket immunoelectrophoresis. In cells sporulating in nutrient broth, the protease antigen levels increased about 7-fold, whereas the specific activity increased about 150-fold, for an activation of about 20-fold. In cells sporulating in the single chemically defined sporulation medium, the protease antigen increased about 10-fold, whereas the specific activity increased at least 400-fold, for an activation of about 40-fold. These results were interpreted to mean that a posttranslational event activated the protease in vivo; a previously described endogenous proteinase inhibitor was confirmed to be present in the strain used. Chloramphenicol added to the cultures inhibited both the increases in antigen levels and in the specific activity of the proteinase.
authors.forename: T J
authors.initials: TJ
authors.lastname: Burnett
authors.forename: G W
authors.initials: GW
authors.lastname: Shankweiler
authors.forename: J H
authors.initials: JH
authors.lastname: Hageman
mesh_terms: D000818:Animals; D001412:Bacillus subtilis / Q000201:enzymology* / Q000502:physiology; D002701:Chloramphenicol / Q000494:pharmacology; D003470:Culture Media; D010450:Endopeptidases / Q000032:analysis* / Q000276:immunology; D004789:Enzyme Activation; D011480:Protease Inhibitors; D011817:Rabbits; D012697:Serine Endopeptidases; D013171:Spores, Bacterial
chemical_list: D003470:Culture Media; D011480:Protease Inhibitors; D002701:Chloramphenicol; D010450:Endopeptidases; D012697:Serine Endopeptidases