Platelet-leukocyte interaction: selective binding of thrombin-stimulated platelets to human monocytes, polymorphonuclear leukocytes, and related cell lines.
The association of platelets with leukocytes was investigated, using gel-filtered platelets stimulated with thrombin and then fixed with formaldehyde. Evidence is presented that stimulation of gel-filtered platelets with low concentrations of thrombin (0.01 to 0.1 U/mL) induces the expression of surface determinants interacting strongly with monocytes and polymorphonuclear leukocytes (PMNs) but only weakly with lymphocytes. Both monocyte-platelet binding and PMN-platelet binding occurred at 37 degrees C and more intensively at 0 degrees C; it was Ca2+-dependent and was unaffected by the addition of sodium azide. The binding also occurred with the monocytoid cell lines HL 60 and U 937 in exponential growth and was much less two days after induction of terminal differentiation by phorbol myristate acetate (PMA). No other tested cell lines (B cells, T cells, and myeloid cells) bound thrombin-stimulated platelets. Monocyte-derived macrophages kept in culture for one week also exhibited reduced binding of thrombin-stimulated platelets. IgG and fibronectin could be ruled out as ligands that mediate binding.
Jungi
T W
TW
Spycher
M O
MO
Nydegger
U E
UE
Barandun
S
S
D001792:Blood Platelets / Q000166:cytology*; D002118:Calcium / Q000502:physiology; D002448:Cell Adhesion; D002450:Cell Communication; D002460:Cell Line; D004492:Edetic Acid / Q000494:pharmacology; D005353:Fibronectins / Q000502:physiology; D006801:Humans; D007074:Immunoglobulin G / Q000502:physiology; D009000:Monocytes / Q000166:cytology*; D009504:Neutrophils / Q000166:cytology*; D010974:Platelet Aggregation; D013917:Thrombin / Q000494:pharmacology*
D005353:Fibronectins; D007074:Immunoglobulin G; D004492:Edetic Acid; D013917:Thrombin; D002118:Calcium