Non-immune human sera at higher concentrations inhibit Plasmodium falciparum growth in vitro.
Plasmodium falciparum growth in vitro related to the concentration of inactivated, non-immune human serum supplement to the RPMI medium. This study investigated the concentration of non-immune serum required to support adequate in vitro parasite growth without saturating the medium. Parasitaemia was highest with 7.5% serum concentration in suspension cultures. However, peak parasitaemia obtained under static cultures with 12.5% serum concentration did not significantly differ from the level attained with suspension cultures at the same serum concentration. Ten per cent serum-supplemented medium supported parasite growth in static and suspension cultures, and levels of parasitaemia declined with further increases in serum concentration.
Golightly
E K
EK
Medical Research Council Laboratories, Fajara, The Gambia.
Greenwood
B M
BM
D000818:Animals; D001790:Blood Physiological Phenomena; D003470:Culture Media; D010963:Plasmodium falciparum / Q000254:growth & development*
D003470:Culture Media