title: The effect of parenteral nutrition on biliary calcium and bilirubin.
abstract: Recent studies have suggested that patients maintained on prolonged total parenteral nutrition (TPN) are at increased risk for gallstone formation. Animal and human data suggest that TPN causes calcium bilirubinate sludge and pigment gallstones. However, the effect of TPN on bile bilirubin and calcium concentrations has not previously been investigated. We, therefore, tested the hypothesis that TPN alters biliary bilirubin and calcium. Eight adult male prairie dogs received TPN (dextrose 15%, FreAmine III 4.25%, Intralipid 10%, insulin 25 U, electrolytes, and vitamins) at a rate of 80 cc/kg/day for 10 days. Eight additional animals maintained on a trace cholesterol diet served as controls. Gallbladder and hepatic bile samples were assayed for bilirubin and calcium. Cholesterol saturation index (CSI) and Rsa, a measure of gallbladder stasis, were also calculated. Calcium bilirubinate crystals were seen in gallbladder bile or wall scrapings of 7 of 8 TPN animals but in none of the controls (P less than 0.001). Animals that received TPN had bilirubin levels that were significantly higher in gallbladder bile (24.8 +/- 7.0 vs 5.1 +/- 0.9, P less than 0.05). Similarly, TPN animals had calcium levels that were significantly higher in gallbladder (30.0 +/- 3.6 vs 13.9 +/- 2.1, P less than 0.005) as well as hepatic (10.5 +/- 0.6 vs 7.4 +/- 0.6, P less than 0.005) bile. The Rsa values were also significantly lower (P less than 0.01) in TPN animals (0.57 +/- 0.07 vs 1.06 +/- 0.14). CSI, serum bilirubin, serum calcium, hematocrit, and reticulocyte counts did not differ between groups.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.forename: E L
authors.initials: EL
authors.lastname: Muller
authors.forename: P A
authors.initials: PA
authors.lastname: Grace
authors.forename: H A
authors.initials: HA
authors.lastname: Pitt
mesh_terms: D000818:Animals; D001646:Bile / Q000378:metabolism*; D001647:Bile Acids and Salts / Q000378:metabolism; D001663:Bilirubin / Q000378:metabolism*; D001774:Blood Chemical Analysis; D001835:Body Weight; D002118:Calcium / Q000378:metabolism*; D002452:Cell Count; D002769:Cholelithiasis / Q000209:etiology; D002784:Cholesterol / Q000378:metabolism; D003460:Crystallization; D005704:Gallbladder / Q000378:metabolism; D006400:Hematocrit; D050356:Lipid Metabolism; D008099:Liver / Q000033:anatomy & histology / Q000378:metabolism; D008297:Male; D009929:Organ Size; D010289:Parenteral Nutrition, Total; D012156:Reticulocytes / Q000166:cytology; D012589:Sciuridae
chemical_list: D001647:Bile Acids and Salts; D002784:Cholesterol; D001663:Bilirubin; D002118:Calcium