An adaptive response to alkylating agents in Aspergillus nidulans.
A simple method is described for demonstrating adaptation to alkylation damage in Aspergillus nidulans. One wild type, two MNNG-sensitive, and one MNNG-resistant strain all showed improvement in colony growth when challenged with MNNG following appropriate inducing pretreatments. Other alkylating agents (MMS, EMS) could also adapt mycelium to later MNNG challenge, while 4NQO and UV could not. The inducible effect was not transmissible through conidia. A standard reversion assay based upon methG proved impractical for studying mutation frequencies during alkylation treatments owing to variations in MNNG resistance amongst revertants.
Hooley
P
P
Department of Genetics, University of Liverpool, UK.
Shawcross
S G
SG
Strike
P
P
D000222:Adaptation, Physiological; D000477:Alkylating Agents / Q000494:pharmacology*; D001233:Aspergillus nidulans / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D004352:Drug Resistance, Microbial / Q000235:genetics; D008722:Methods; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology*; D009153:Mutagens / Q000494:pharmacology; D009154:Mutation; D014466:Ultraviolet Rays
D000477:Alkylating Agents; D009153:Mutagens; D008769:Methylnitronitrosoguanidine