Potentiation of glucose-induced insulin release in islets by desHis1[Glu9]glucagon amide.
Glucagon and secretin and some of their hybrid analogs potentiate glucose-induced release of insulin from isolated mouse pancreatic islets. It was recently shown that the synthetic glucagon analog, desHis1[Glu9]glucagon amide, does not stimulate the formation of cyclic adenosine monophosphate in the rat hepatocyte membrane, but binds well to the glucagon receptor and is a good competitive antagonist of glucagon. In the present study the effect of this analog on isolated islets was examined. desHis1-[Glu9]glucagon amide at 3 x 10(-7) M, in the presence of 0.01 M D-glucose, increased the release of insulin by 30% and maintained that level for the full 30-min test period. The rate of insulin release returned to the glucose-induced base line after removal of the peptide. The same insulin level was produced by 3 x 10(-9) M glucagon, and at 3 x 10(-7) M glucagon insulin release was enhanced 290% above the glucose base line.
Kofod
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Hagedorn Research Laboratory, Gentofte, Denmark.
Unson
C G
CG
Merrifield
R B
RB
D000595:Amino Acid Sequence; D000818:Animals; D004357:Drug Synergism; D005934:Glucagon / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000494:pharmacology; D005947:Glucose / Q000494:pharmacology*; D066298:In Vitro Techniques; D007202:Indicators and Reagents; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000187:drug effects / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains
D007202:Indicators and Reagents; D007328:Insulin; C059548:glucagon amide, des-His(1)-; D005934:Glucagon; D005947:Glucose