Preservation of red blood cells: content of microaggregates and di-2-ethylhexylphthalate (DEHP) in red blood cells stored in saline-adenine-glucose-mannitol (SAGM) medium.
The content of microaggregates was determined in leukocyte-poor red blood cells (RBC) after 5 weeks of storage in citrate-phosphate-dextrose (CPD)-adenine plasma and in saline-adenine-glucose-mannitol (SAGM) medium. The size range was 3,600-200,000 fl, corresponding to a particle diameter of 19-73 micron, presuming a spherical particle shape. In comparison with CPD-adenine whole blood (100%), the total number of particles per unit of RBC was found to be 41.2% (CPD-adenine anticoagulant) and 25.4% (SAGM medium). The corresponding data for total particle volumes were 43.3 and 30.3%. The significant differences between the two categories of RBC could not be explained on the basis of differences concerning pH or cell composition. A majority of particles (50-62%) was observed in the lowest size range (19-29 microns), corresponding to only 18-26% of the total particle volume. The accumulation of di-2-ethylhexylphthalate (DEHP) was determined in two variations of RBC stored in SAGM medium and in RBC stored in CPD-adenine plasma. The quantities of DEHP per unit of RBC after 5 weeks were significantly smaller in SAGM medium (9.0 +/- 3.6 and 22.7 +/- 6.1 mg, respectively) compared to CPD-adenine plasma (30.1 +/- 9.5 mg).
Gulliksson
H
H
Karlman
G
G
Segerlind
A
A
Gullbring
B
B
D000225:Adenine; D001793:Blood Preservation; D003470:Culture Media; D004051:Diethylhexyl Phthalate / Q000097:blood*; D004903:Erythrocyte Aggregation; D004912:Erythrocytes / Q000032:analysis / Q000166:cytology*; D005947:Glucose; D006400:Hematocrit; D006801:Humans; D007962:Leukocytes / Q000166:cytology; D008353:Mannitol; D010795:Phthalic Acids / Q000097:blood*; D012965:Sodium Chloride; D013997:Time Factors
D003470:Culture Media; D010795:Phthalic Acids; D008353:Mannitol; D012965:Sodium Chloride; D004051:Diethylhexyl Phthalate; D005947:Glucose; D000225:Adenine