title: HIV expression strategies: ribosomal frameshifting is directed by a short sequence in both mammalian and yeast systems.
abstract: The pol gene of the human immunodeficiency virus (HIV-1) is expressed as a gag:pol fusion, arising from a ribosomal frameshift that brings the overlapping, out-of-phase gag and pol genes into translational phase. In this study, we show that HIV frameshifting is mediated by a very short sequence in the viral RNA. We demonstrate the importance of a homopolymeric run within this sequence and conclude that HIV frameshifting is not dependent on stem-loop structures downstream from the frameshift site. Our analysis also indicates that the sequence requirements are identical in mammalian and yeast systems.
authors.affiliation: Department of Biochemistry, University of Oxford, England.
authors.forename: W
authors.initials: W
authors.lastname: Wilson
authors.forename: M
authors.initials: M
authors.lastname: Braddock
authors.forename: S E
authors.initials: SE
authors.lastname: Adams
authors.forename: P D
authors.initials: PD
authors.lastname: Rathjen
authors.forename: S M
authors.initials: SM
authors.lastname: Kingsman
authors.forename: A J
authors.initials: AJ
authors.lastname: Kingsman
mesh_terms: D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D015497:HIV-1 / Q000235:genetics*; D008322:Mammals; D009690:Nucleic Acid Conformation; D010957:Plasmids; D014176:Protein Biosynthesis; D012367:RNA, Viral / Q000032:analysis; D011993:Recombinant Fusion Proteins / Q000032:analysis; D012270:Ribosomes; D012441:Saccharomyces cerevisiae / Q000235:genetics
chemical_list: D012367:RNA, Viral; D011993:Recombinant Fusion Proteins