Hormonal regulation of muscle protein catabolism in acutely uremic rats: effect of adrenalectomy and parathyroidectomy.
To examine the role of glucocorticoids and PTH on the enhanced protein catabolism of acute uremia, rats were rendered uremic and had their adrenals or their parathyroid glands concomitantly removed. Adrenalectomy resulted in a marked reduction of urea generation in uremic animals due to decrease of myofibrillar protein breakdown as indicated by lower serum levels of Nt-methylhistidine and a reduction in the activity of the myofibrillar proteinase from skeletal muscle. This reduced urea formation was accompanied by marked hypoglycemia. Parathyroidectomy, on the other hand, caused no change of those parameters of protein catabolism, suggesting that PTH does not account for the protein degradation observed in acutely uremic rats.
Schaefer
R M
RM
Department of Internal Medicine, University of Wuerzburg, FRG.
Moser
M
M
Kulzer
P
P
Peter
G
G
Heidland
A
A
Hörl
W H
WH
Massry
S G
SG
D000208:Acute Disease; D000315:Adrenalectomy; D000818:Animals; D001774:Blood Chemical Analysis; D010450:Endopeptidases / Q000032:analysis; D006728:Hormones / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D008762:Methylhistidines / Q000097:blood; D009124:Muscle Proteins / Q000378:metabolism*; D009132:Muscles / Q000201:enzymology; D009392:Nephrectomy; D010280:Parathyroid Glands / Q000502:physiology / Q000601:surgery; D051381:Rats; D011919:Rats, Inbred Strains; D014511:Uremia / Q000378:metabolism*
D006728:Hormones; D008762:Methylhistidines; D009124:Muscle Proteins; D010450:Endopeptidases