Protein blotting: techniques and application in virus hepatitis research.
The protein-blotting technique is a very practical way to detect and characterize certain proteins in a complex sample by various reagents like polyclonal and monoclonal antibodies, lectins, nucleic acids, or other ligands. This article discusses several options to perform this technique with special reference to viral hepatitis. We give a detailed description of denaturing gel electrophoresis, blotting and specific detection methods. At the beginning of each section a general outline of a certain technique is given, thereafter the reagents, equipment, suppliers, and techniques are described in a way that a beginner may be able to perform the assay.
Heermann
K H
KH
Department of Medical Microbiology, University of Göttingen.
Gültekin
H
H
Gerlich
W H
WH
D000818:Animals; D015139:Blotting, Southern / Q000295:instrumentation / Q000379:methods*; D015153:Blotting, Western / Q000295:instrumentation / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel / Q000295:instrumentation / Q000379:methods*; D006524:Hepatitis, Viral, Animal / Q000235:genetics*; D006525:Hepatitis, Viral, Human / Q000235:genetics*; D006801:Humans; D012967:Sodium Dodecyl Sulfate
D012967:Sodium Dodecyl Sulfate