A kinetic analysis of Drosophila melanogaster dopa decarboxylase.
The kinetic mechanism of dopa decarboxylase (3,4-dihydroxy-L-phenylalanine carboxy-lyase, EC 4.1.1.28) was investigated in Drosophila melanogaster. Based on initial velocity and product inhibition studies, an ordered reaction is proposed for dopa decarboxylase. This kinetic mechanism is interpreted in the context of measured enzyme activities and the catecholamine pools in Drosophila. The 1(2)amd gene is immediately adjacent to the gene coding for dopa decarboxylase (Ddc) and determines hypersensitivity to alpha-methyldopa in Drosophila. Dopa decarboxylase does not decarboxylate alpha-methyldopa and hence does not generate a toxic product capable of inhibiting 1(2)amd gene function. We propose that the 1(2)amd gene is involved with an unknown catecholamine pathway involving dopa but not dopamine.
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D000818:Animals; D065105:Aromatic Amino Acid Decarboxylase Inhibitors; D001142:Aromatic-L-Amino-Acid Decarboxylases / Q000378:metabolism*; D002395:Catecholamines / Q000378:metabolism; D004296:Dopa Decarboxylase / Q000235:genetics / Q000378:metabolism*; D004298:Dopamine / Q000031:analogs & derivatives / Q000494:pharmacology; D004331:Drosophila melanogaster / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D005796:Genes; D007700:Kinetics; D008345:Manganese / Q000494:pharmacology; D008750:Methyldopa / Q000378:metabolism / Q000633:toxicity; D015032:Zinc / Q000494:pharmacology
D065105:Aromatic Amino Acid Decarboxylase Inhibitors; D002395:Catecholamines; C006374:N-acetyldopamine; D008345:Manganese; D008750:Methyldopa; D004296:Dopa Decarboxylase; D001142:Aromatic-L-Amino-Acid Decarboxylases; D015032:Zinc; D004298:Dopamine