Human cysteine proteinase inhibitors. Isolation, physiological importance, inhibitory mechanism, gene structure and relation to hereditary cerebral hemorrhage.
The isolation and characterization of six human cysteine proteinase inhibitors is reported. Their distribution in human biological fluids is also described and discussed with respect to physiological function. Studies on kininogen and cystatin C with respect to structure-function relationships and, as a result of the cystatin C studies, a general model for the mechanism of cysteine proteinase inhibition by cystatins are presented. The model was used for the construction of synthetic inhibitors which showed good inhibitory properties against papain and the streptococcal cysteine proteinase. Structures of cDNA and gene for normal human cystatin C are accounted for, as well as studies on the cystatin C gene in patients suffering from hereditary cystatin C amyloid angiopathy (HCCAA). As a result of this an RFLP that showed total co-segregation with the disease was found. It was concluded that the disease is caused by a point mutation in the cystatin C structural gene and that the RFLP will be a most useful tool for diagnosis of HCCAA. The production of recombinant cystatin C in E. coli is also reported and its possible use for treatment of HCCAA is discussed.
Abrahamson
M
M
Department of Clinical Chemistry, University of Lund, Malmö General Hospital, Sweden.
D000595:Amino Acid Sequence; D001483:Base Sequence; D001826:Body Fluids / Q000201:enzymology; D002543:Cerebral Hemorrhage / Q000201:enzymology / Q000235:genetics; D015853:Cysteine Proteinase Inhibitors; D004247:DNA / Q000235:genetics; D005796:Genes; D006801:Humans; D007700:Kinetics; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011480:Protease Inhibitors / Q000502:physiology*
D015853:Cysteine Proteinase Inhibitors; D011480:Protease Inhibitors; D004247:DNA