title: [Microbiological determination of DNA levels in blood leukocytes].
abstract: A procedure is developed for estimation of DNA microquantities in biological samples, which involved acid hydrolysis of DNA and the following microbiological estimation of its concentration. A sensitivity of the procedure was as little as 0.25 micrograms DNA per ml. At the DNA concentration 0.25-10 micrograms/ml growth of the indicator strain of bacteria exhibited the linear shape. As shown by means of the procedure developed one rat leukocyte contained 6.3 +/- 0.6 X 10(-12) g of DNA.
authors.forename: K O
authors.initials: KO
authors.lastname: Blokh
authors.forename: I F
authors.initials: IF
authors.lastname: Paskevich
mesh_terms: D000818:Animals; D001681:Biological Assay; D004247:DNA / Q000032:analysis*; D006868:Hydrolysis; D007779:Lactobacillus acidophilus / Q000254:growth & development; D007962:Leukocytes / Q000032:analysis*; D008297:Male; D008828:Microbiological Techniques; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D004247:DNA
vernacular_title: Mikrobiologicheskoe opredelenie kontsentratsii DNK v leĭkotsitakh krovi.