The effect of high glucose and high insulin concentrations on pentose phosphate shunt enzymes and malic enzyme in cultured human endothelial cells.
The influence of glucose and insulin on pentose phosphate shunt enzymes and malic enzyme activity in cultured human endothelial cells has been investigated. Glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase and malic enzyme were present in endothelial cells. Enzyme activities were not altered either by 20 mM glucose or 10(-8) M insulin after 3, 6 and 12 hour incubations respectively. Neither increased glucose nor increased insulin alter the activity of the pentose phosphate shunt. As a consequence fatty acid and cholesterol synthesis in the endothelial cell is unlikely to be altered in the presence of increased glucose or increased insulin.
Hawthorne
G C
GC
Department of Medicine, Medical School, Newcastle upon Tyne, United Kingdom.
Alberti
K G
KG
D004730:Endothelium, Vascular / Q000201:enzymology*; D005947:Glucose / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D007328:Insulin / Q000494:pharmacology*; D008291:Malate Dehydrogenase / Q000378:metabolism*; D010427:Pentose Phosphate Pathway / Q000187:drug effects*
D007328:Insulin; D008291:Malate Dehydrogenase; D005947:Glucose