Development of a sensitive enzyme-linked immunosorbent assay for the detection of diacetoxyscirpenol.
An enzyme-linked immunosorbent assay (ELISA) for the detection of diacetoxyscirpenol (DAS) was developed. Antibodies against DAS were obtained after immunization of rabbits with DAS-hemiglutarate-human serum albumin (DAS-HG-HSA). After an immunization period of 6 weeks a sufficient level of specific antibodies was reached. A DAS-hemisuccinate-horseradish peroxidase-conjugate (DAS-HS-HRP) was prepared by an ester method and used as enzyme-labelled toxin in the competitive assay. The detection limit for DAS was about 10 pg/ml. The relative cross reactivity of the assay was 597.5, 5.2, 100.0, 2.5, and 1.5% for 3 alpha-acetyl-DAS, DAS, T-2 toxin, neosolaniol, and 15-acetoxyscirpenol, respectively.
Klaffer
U
U
Lehrstuhl für Hygiene und Technologie der Milch, München, F.R.G.
Märtlbauer
E
E
Terplan
G
G
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D001667:Binding, Competitive; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D005658:Fungi / Q000032:analysis; D009183:Mycotoxins / Q000032:analysis*; D011817:Rabbits; D012680:Sensitivity and Specificity; D012717:Sesquiterpenes / Q000032:analysis*; D014255:Trichothecenes / Q000032:analysis* / Q000276:immunology
D000911:Antibodies, Monoclonal; D009183:Mycotoxins; D012717:Sesquiterpenes; D014255:Trichothecenes; C001809:diacetoxyscirpenol