Autophosphorylation of yeast hexokinase PII.
Autophosphorylation of hexokinase PII was studied using an enzyme purified from Saccharomyces cerevisiae. Incubation of this enzyme preparation with [gamma-32P]ATP and Mn2+ or Mg2+ gave a phosphoprotein of molecular mass 58,000 which corresponded to hexokinase PII. D-Xylose stimulated autophosphorylation of hexokinase PII. Dilution of hexokinase PII over a 10-fold concentration range did not change the specific activity of hexokinase PII autophosphorylation suggesting that it may occur by an intramolecular mechanism.
Fernández
R
R
Departamento de Biología Funcional, Facultad de Medicina, Universidad de Oviedo, Spain.
Herrero
P
P
Fernández
E
E
Fernández
T
T
López-Boado
Y S
YS
Moreno
F
F
D006593:Hexokinase / Q000378:metabolism*; D008274:Magnesium / Q000494:pharmacology; D008345:Manganese / Q000494:pharmacology; D010085:Oxidative Phosphorylation / Q000187:drug effects; D010750:Phosphoproteins / Q000096:biosynthesis; D010766:Phosphorylation; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000378:metabolism; D013997:Time Factors; D014994:Xylose / Q000494:pharmacology
D010750:Phosphoproteins; D008345:Manganese; D014994:Xylose; D006593:Hexokinase; D008274:Magnesium