[Hormone receptor determination in breast cancer using the dextran- coated charcoal method and monoclonal antibodies: correlation with cytomorphologic grading].
In 129 cases, results from ER-ICA (Estrogen receptor immunocytochemical assay) applied to breast carcinoma biopsy smears and results from ER-EIA (Estrogen receptor enzyme immunoassay) applied to cytosols of breast carcinoma were compared to the results of ER(DCC), PR(DCC) and a cytomorphological grading. Using 500x magnification, the cases were graded visually in 7 groups, groups 1-4 without and groups 5-7 with nucleoli. Cases with high estrogen receptor values as determined by ER-EIA had the best chance of being detected also by the ER-ICA method. There is a relation between the visual nuclear grade from the Pappenheim stained specimens and ER-ICA and ER-EIA. The 48 cases without nucleoli had a mean ER-EIA-value of 100 fmol/mg (median = 59 fmol/mg). The 57 cases with nucleoli (groups 5-7) had a mean ER-EIA value of 37 fmol/mg (median = 14 fmol/mg). For 28 cases with prominent or multiple prominent nucleoli (groups 6-7) the mean ER-EIA value was only 15 fmol/mg (median = 5 fmol/mg). With increasing ER-EIA values we found decreasing average nuclear grades: 31 cases ER-EIA less than 10 fmol/mg, mean nuclear grade = 5.4; 34 cases ER-EIA greater than or equal to 10 less than 50 fmol/mg, mean nuclear grade = 4.6; 20 cases ER-EIA greater than or equal to 50 less than 100 fmol/mg, mean nuclear grade = 4.2; 19 cases ER-EIA greater than or equal to 100 fmol/mg, mean nuclear grade = 3.8. Our present data show clearly that there is a closer correlation of cytomorphology with ER-EIA than with ER(DCC) and PR(DCC). In the present study even ER-EIA alone correlated better with morphology than ER(DCC) and PR(DCC) taken together.
Schenck
U
U
Institut für Klinische Zytologie der Technischen Universität München.
Jütting
U
U
Eiermann
W
W
D000911:Antibodies, Monoclonal; D001940:Breast / Q000473:pathology; D001943:Breast Neoplasms / Q000473:pathology*; D002466:Cell Nucleolus / Q000648:ultrastructure; D002467:Cell Nucleus / Q000648:ultrastructure; D003600:Cytosol / Q000648:ultrastructure; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D009367:Neoplasm Staging; D009376:Neoplasms, Hormone-Dependent / Q000473:pathology*; D011379:Prognosis; D011960:Receptors, Estrogen / Q000032:analysis*; D011980:Receptors, Progesterone / Q000032:analysis*
D000911:Antibodies, Monoclonal; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone
Hormonrezeptorbestimmung beim Mammakarzinom mit der Dextran-Coated-Charcoal-Methode und mit monoklonalen Antikörpern: Korrelation mit einem zytomorphologischen Grading.