LR-White and LR-Gold resins for postembedding immunofluorescence staining of laminin in mouse kidney.
This work describes a method for the immunolocalization of laminin on 1 micron-thick tissue sections using a postembedding immunofluorescence technique. Embedding of unfixed or formaldehyde-fixed mouse renal cortex in either of the acrylic resins LR-White or LR-Gold permitted reliable postembedding immunofluorescence staining for laminin. LR-White was heat-cured at 50 degrees C whereas LR-Gold was polymerized at -25 degrees C. A stronger immunostaining for laminin was obtained from tissue embedded in polymerized LR-Gold compared with the staining from tissue embedded in LR-White. Prerequisites for adequate postembedding immunostaining are the partial dehydration of the tissue (maximum ethanol concentration, 70%) and pepsin treatment of the tissue sections prior to performing the immunostaining reactions.
Herken
R
R
Abteilung Histologie, Georg-August Universität Göttingen, Germany.
Fussek
M
M
Barth
S
S
Götz
W
W
D000180:Acrylic Resins; D000818:Animals; D005260:Female; D005455:Fluorescent Antibody Technique; D007150:Immunohistochemistry / Q000379:methods; D007668:Kidney / Q000032:analysis*; D007797:Laminin / Q000032:analysis* / Q000276:immunology; D051379:Mice
D000180:Acrylic Resins; D007797:Laminin; C048707:LR white; C056756:LR gold