A new member of the ras gene superfamily identified in a rat liver cell line.
A new member of the ras genes superfamily was isolated from a cDNA library derived from a rat liver cell line (BRL-3A). The predicted 201 amino acids ras-like protein shows 30-35% homology with other members of the ras and ras-related gene products so far described. Conserved features include the GTP-binding and hydrolysis domains and the carboxyl terminal cysteine residues. A protein of the expected size (Mr 23,000) was synthesized in an in vitro transcription-translation system. The BRL-ras gene is present in single copy in the rat genome and is ubiquitously expressed at high levels in all tissues and cell lines examined.
Bucci
C
C
Dipartimento di Biologia e Patologia Cellulare e Molecolare, Università di Napoli, Italy.
Frunzio
R
R
Chiariotti
L
L
Brown
A L
AL
Rechler
M M
MM
Bruni
C B
CB
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D011905:Genes, ras; D008099:Liver; D008969:Molecular Sequence Data; D011487:Protein Conformation; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D016283:Proto-Oncogene Proteins p21(ras); D051381:Rats; D012689:Sequence Homology, Nucleic Acid
D011518:Proto-Oncogene Proteins; D016283:Proto-Oncogene Proteins p21(ras)