title: The detection of a spectrin-like protein in Trypanosoma cruzi with a polyclonal antibody.
abstract: A rabbit serum raised against sheep erythrocyte spectrin stained mainly the flagella of epimastigote forms of the parasitic protozoan Trypanosoma cruzi. In Western blots of T. cruzi proteins solubilized with Nonidet P-40 it recognized mainly a polypeptide doublet with an apparent molecular weight of about 240 kDa, while a pre-immune rabbit serum and a tubulin monoclonal antibody did not. The results are consistent with the idea that a spectrin-like protein may be involved in cytoskeleton-membrane interactions in flagella of T. cruzi.
authors.affiliation: Centro de Biologia Molecular (CSIC-UAM), Universidad Autonoma, Madrid, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Alcina
authors.forename: A J
authors.initials: AJ
authors.lastname: Hargreaves
authors.forename: J
authors.initials: J
authors.lastname: Avila
authors.forename: J E
authors.initials: JE
authors.lastname: Hesketh
authors.forename: M
authors.initials: M
authors.lastname: Fresno
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology*; D003429:Cross Reactions; D003599:Cytoskeleton / Q000032:analysis; D005455:Fluorescent Antibody Technique; D015151:Immunoblotting; D008970:Molecular Weight; D013049:Spectrin / Q000032:analysis*; D014349:Trypanosoma cruzi / Q000032:analysis*
chemical_list: D000906:Antibodies; D013049:Spectrin