title: A chromosomal mutation mediating increased expression of pyrE in Salmonella typhimurium is located within the proposed attenuator.
abstract: A chromosomal mutation resulting in 15- to 20-fold increased expression of the Salmonella typhimurium pyrE gene has been cloned and sequenced. The mutation was found to be a single base-pair transition of GC to AT, and occurred within a region of dyad symmetry (attenuator) located just upstream of the pyrE structural gene.
authors.affiliation: Enzyme Division, University Institute of Biological Chemistry B, Copenhagen K, Denmark.
authors.forename: J
authors.initials: J
authors.lastname: Neuhard
authors.forename: R A
authors.initials: RA
authors.lastname: Kelln
mesh_terms: D001483:Base Sequence; D002876:Chromosomes, Bacterial; D003001:Cloning, Molecular; D005786:Gene Expression Regulation; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D009154:Mutation; D009962:Orotate Phosphoribosyltransferase / Q000096:biosynthesis / Q000235:genetics*; D010430:Pentosyltransferases / Q000235:genetics*; D010957:Plasmids; D012486:Salmonella typhimurium / Q000201:enzymology / Q000235:genetics*
chemical_list: D010430:Pentosyltransferases; D009962:Orotate Phosphoribosyltransferase