Comparison of a rapid latex agglutination assay and a fluorescent-antibody technique for the detection of herpes simplex antibody.
A total of 202 serum specimens was tested for the presence of herpes simplex virus antibody using a premarket latex agglutination kit (Wampole) and an indirect fluorescent antibody (IFA) technique (electronucleonics). Discrepant results between the two assays were resolved using an Enzyme-linked immunosorbent assay (ELISA) procedure. The overall sensitivity of the latex was 99.2% with a specificity of 98.5%. The latex agglutination test evaluated is a viable alternative to indirect immunofluorescence for the detection of herpes simplex virus antibody in serum samples.
LeBar
W D
WD
Providence Hospital, Southfield, Michigan.
Resek
C M
CM
Crist
A E
AE
Sautter
R L
RL
D000372:Agglutination Tests; D000914:Antibodies, Viral / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006561:Herpes Simplex / Q000276:immunology*; D006801:Humans; D007840:Latex; D011897:Random Allocation; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity
D000914:Antibodies, Viral; D007840:Latex; D011933:Reagent Kits, Diagnostic