Inhibition by auranofin of pharmacologic and antigen-induced contractions of the isolated guinea pig trachea.
The effects of an orally active gold complex, auranofin, were investigated on the isolated guinea pig trachea contracted pharmacologically and antigenically. Pretreatment of the tracheal tissues for 15 minutes with auranofin (10(-5) and 10(-4) mol/L) produced a significant rightward shift of a histamine dose-response curve (p less than 0.001), whereas a 30-minute pretreatment with auranofin (10(-4) mol/L) inhibited LTD4-induced (but not LTC4-induced) contraction of the tracheal spirals. In tracheas from animals actively sensitized to ovalbumin, auranofin (10(-4) mol/L) significantly inhibited contractions elicited by 0.01 micrograms/ml of this antigen. Auranofin does not appear to behave like a nonspecific suppressant of tracheal muscle contraction since it failed to antagonize a potassium chloride-induced (6 X 10(-2) mol/L) contraction. Therefore, auranofin appears to alter the in vitro response of the guinea pig trachea not only to histamine but also, more importantly, to LTD4 and specific antigen. These results characterize and extend further the pharmacology of auranofin in airway tissue.
Malo
P E
PE
Wasserman
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M
Parris
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D
Pfeiffer
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D
D000818:Animals; D000941:Antigens / Q000494:pharmacology*; D001310:Auranofin; D006051:Aurothioglucose / Q000031:analogs & derivatives* / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006046:Gold / Q000031:analogs & derivatives*; D006168:Guinea Pigs; D006632:Histamine / Q000494:pharmacology; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D010047:Ovalbumin / Q000494:pharmacology; D013189:SRS-A / Q000494:pharmacology; D014132:Trachea / Q000502:physiology*
D000941:Antigens; D013189:SRS-A; D006051:Aurothioglucose; D001310:Auranofin; D006046:Gold; D006632:Histamine; D010047:Ovalbumin