Evaluation of simultaneous measurement of lutropin and follitropin with the SimulTROPIN radioimmunoassay kit.
We evaluated the analytical performance of the "SimulTROPIN" (Becton-Dickinson Inc.) radioimmunoassay for the simultaneous measurement of lutropin (LH) and follitropin (FSH) in human serum. Dose response, linearity, analytical recovery, sensitivity, and reagent stability were all acceptable. Cross reactivity with other structurally related hormones [choriogonadotropin (CG) and thyrotropin (TSH)] was minimal. A normal reference interval was established for nonmidcycle, ovulatory women. We confirm the low degree of CG and TSH cross reactivity with LH and FSH reported by Becton Dickinson and conclude that this assay is an acceptable method for laboratory use in the simultaneous quantification of these analytes.
Wians
F H
FH
Dev
J
J
Powell
M M
MM
Heald
J I
JI
D000293:Adolescent; D000328:Adult; D006063:Chorionic Gonadotropin / Q000097:blood; D003429:Cross Reactions; D005260:Female; D005640:Follicle Stimulating Hormone / Q000097:blood*; D006801:Humans; D007986:Luteinizing Hormone / Q000097:blood*; D008597:Menstrual Cycle; D011863:Radioimmunoassay; D011933:Reagent Kits, Diagnostic; D012016:Reference Values; D013972:Thyrotropin / Q000097:blood
D006063:Chorionic Gonadotropin; D011933:Reagent Kits, Diagnostic; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D013972:Thyrotropin