Neuroanatomical localization of cells containing gonadotropin-releasing hormone messenger ribonucleic acid in the primate brain by in situ hybridization histochemistry.
Using in situ hybridization histochemistry, we have mapped the anatomic localization of perikarya containing mRNA that codes for GnRH and GnRH-associated protein (GAP) in the forebrain of four male macaques, Macaca fascicularis. DNA oligomers, with sequences complementary to either the GnRH or the GAP portion of the mRNA sequence, were synthesized and hybridized to paraformaldehyde fixed, coronal sections of the basal forebrain and hypothalamus. GnRH mRNA was found in the same population of cells as those containing GAP mRNA. GnRH/GAP mRNA-containing cell bodies were observed consistently in the medial septal nucleus, the diagonal band of Broca, the medial preoptic area, supraoptic nucleus, and ventromedial-infundibular region. We detected the presence of GnRH mRNA and GAP mRNA within the same neuroanatomic regions previously shown to include perikarya containing immunoreactive GnRH. The ventromedial-infundibular region and the medial preoptic region contained the greatest number of GnRH/GAP mRNA-containing perikarya (37.0% and 22.5%, respectively). The diagonal band contained 21.0% and the supraoptic nucleus 13.0% of the cells, while the medial septum contained the fewest number (6.7%). This study demonstrates the feasibility of using in situ hybridization as a strategy to study the developmental and steroidal regulation of GnRH gene expression in the nonhuman primate.
Standish
L J
LJ
Department of Obstetrics and Gynecology, University of Washington, Seattle 98195.
Adams
L A
LA
Vician
L
L
Clifton
D K
DK
Steiner
R A
RA
D000818:Animals; D001345:Autoradiography; D001483:Base Sequence; D001921:Brain / Q000033:anatomy & histology* / Q000166:cytology; D007987:Gonadotropin-Releasing Hormone / Q000235:genetics*; D006651:Histocytochemistry; D008252:Macaca fascicularis; D008297:Male; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D011498:Protein Precursors / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics*; D013462:Sulfur Radioisotopes
D015345:Oligonucleotide Probes; D011498:Protein Precursors; D012333:RNA, Messenger; D013462:Sulfur Radioisotopes; C050502:gonadotropin releasing hormone associated peptide; D007987:Gonadotropin-Releasing Hormone