Expression of MF alpha 1 in MATa cells supersensitive to alpha-factor leads to self-arrest.
MATa cells of Saccharomyces cerevisiae defective in both the SST1 and SST2 gene products exhibit self-arrest when they express the MF alpha 1 gene under the control of the GAL1 promoter. This response to endogenously produced pheromone can be alleviated by mutations which prevent the production of, or response to, alpha-factor. Suppressors of the self-arrest phenotype include a class of mutants which remain responsive to low levels of pheromone, but are resistant to high levels of alpha-factor. One of these mutants has been mapped to chromosome X, 31 cM distal to SUP4, and defines a new locus designated STE18.
Whiteway
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M
Genetic Engineering Section, NRC Biotechnology Research Institute, Montreal, Que., Canada.
Hougan
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Thomas
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D003433:Crosses, Genetic; D004171:Diploidy; D005786:Gene Expression Regulation; D005800:Genes, Fungal; D000072235:Mating Factor; D009154:Mutation; D010452:Peptide Biosynthesis; D010455:Peptides / Q000235:genetics* / Q000502:physiology; D011401:Promoter Regions, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development; D014170:Transformation, Genetic
D010455:Peptides; D000072235:Mating Factor