A sensitive assay for proteolytic activity using fluorescein-labeled gelatin coupled to Sepharose 4B as substrate.
A method for the determination of proteolytic activity is described which uses fluorescein-labeled gelatin coupled to Sepharose 4B as substrate. The assay is simple and sensitive allowing detection of one nanogram of trypsin and is found suitable for the measurement of gelatinolytic activity in tissue samples.
Tisljar
U
U
Denker
H W
HW
D000818:Animals; D004717:Endometrium / Q000201:enzymology; D005260:Female; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D005780:Gelatin; D010447:Peptide Hydrolases / Q000032:analysis*; D011247:Pregnancy; D011817:Rabbits; D012685:Sepharose; D013050:Spectrometry, Fluorescence; D013379:Substrate Specificity; D013861:Thiocyanates; D014357:Trypsin / Q000032:analysis
D005452:Fluoresceins; D013861:Thiocyanates; D005780:Gelatin; D012685:Sepharose; D010447:Peptide Hydrolases; D014357:Trypsin; D016650:Fluorescein-5-isothiocyanate