[Histamine assay: use of a monoclonal antibody for radioimmunoassay and enzyme immunoassay].
The difficulty in raising highly specific and affined antibodies against histamine lies on the small size of this amine. For this purpose, we developed an original chemical derivatization (acylation) of histamine to induce required antibodies. It is then necessary to reproduce the same chemical modification in all protagonists of the immunoassay: immunogen, tracer and sample. We raised highly specific monoclonal antibodies. The acylation of histamine increases 5.10(5) times its recognition by the monoclonal antibody. We designed a ready for use reagent so no extraction of purification is needed for biological samples. Accurate calibration of monoclonal antibodies fixed on solid phase define the sensitivity of the test. This sensitivity allows histamine determination in plasma samples. The same rationale is applicable whatever tracer is used: either radiolabelled with iodine or coupled to an enzyme.
Delaage
M
M
Immunotech, Marseille.
Darmon
M
M
Morel
A
A
D000911:Antibodies, Monoclonal; D000915:Antibody Affinity; D000918:Antibody Specificity; D001667:Binding, Competitive; D006632:Histamine / Q000032:analysis*; D007124:Immunoenzyme Techniques; D011863:Radioimmunoassay / Q000379:methods*; D013696:Temperature
D000911:Antibodies, Monoclonal; D006632:Histamine
Dosage de l'histamine: utilisation d'un anticorps monoclonal pour un radioimmunoessai et un enzymoimmunoessai.