Reovirus type 1 and type 3 differ in their binding to isolated intestinal epithelial cells.
Binding of reovirus type 1 to dispersed villus cells from the small intestine was found to be specific for the basolateral membrane. Reovirus type 3 did not bind to any surface of small intestinal epithelial cells, but did bind to intra-epithelial lymphocytes. Using reovirus genetic reassortants, it was shown that the viral attachment polypeptide, encoded by the S1 genome segment of reovirus type 1 is essential for reovirus specific immunofluorescence to villus epithelial cells. In addition, the binding of reovirus type 1 to intestinal cells is saturable. Competition for the binding of 125I-labeled reovirus type 1 was demonstrated with unlabeled reovirus type 1 but not unlabeled reovirus type 3. This indicates that the intestinal epithelial receptor for reovirus is not shared by reovirus serotypes 1 and 3, and infection of intestinal epithelial cells by reovirus type 3 may be limited due to a failure of virus to bind.
Weiner
D B
DB
Department of Pathology, University of Pennsylvania, Philadelphia 19104.
Girard
K
K
Williams
W V
WV
McPhillips
T
T
Rubin
D H
DH
D000818:Animals; D002460:Cell Line; D004848:Epithelium / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D005814:Genes, Viral; D006389:Hemagglutinins, Viral / Q000235:genetics / Q000502:physiology; D007422:Intestines / Q000382:microbiology*; D008214:Lymphocytes / Q000382:microbiology; D012089:Mammalian orthoreovirus 3 / Q000235:genetics / Q000472:pathogenicity*; D051379:Mice; D011991:Receptors, Virus / Q000378:metabolism*; D012087:Reoviridae / Q000235:genetics / Q000472:pathogenicity*; D012703:Serotyping; D013045:Species Specificity
D006389:Hemagglutinins, Viral; D011991:Receptors, Virus