[Comparison between immunofluorescence and immunoenzymatic assay for the rapid diagnosis of the respiratory syncytial virus in nasopharyngeal secretions].
An enzyme immunoassay, RSV-EIA Abbot, was evaluated by comparison with indirect immunofluorescence. Nasopharyngeal secretions obtained from 95 infants and young children with acute respiratory infections were examined for the presence of respiratory syncytial virus antigens with both methods. Specimens were stored at -70 degrees C before being tested by EIA. Out of 60 samples positive by indirect immunofluorescence, 46 were also positive by RSV-EIA (sensitivity 78.7%) and 34 out of 35 immunofluorescence negative specimens were negative by RSV-EIA (specificity 97.1%). Therefore, the EIA appears to be an acceptable test for the rapid detection of RSV as an alternative for indirect immunofluorescence.
Chiparelli
H
H
Departamento de Laboratorios, Ministerio de Salud Pública, Montevideo, Uruguay.
Russi
J C
JC
Martorell
E
E
Arbiza
J R
JR
Cánepa
E
E
Hortal
M
M
D000956:Antigens, Viral / Q000032:analysis*; D002648:Child; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006801:Humans; D009093:Mucus / Q000276:immunology*; D009305:Nasopharynx / Q000378:metabolism; D011237:Predictive Value of Tests; D012136:Respiratory Syncytial Viruses / Q000276:immunology*; D010253:Respirovirus Infections / Q000276:immunology*; D013997:Time Factors
D000956:Antigens, Viral
Comparación entre la inmunofluorescencia y el ensayo inmunoenzimático para el diagnóstico rápido del virus respiratorio sincicial en secreciones nasofaríngeas.