title: Temporal and spatial expression of a position specific antigen, AV-1, in chick limb buds.
abstract: In a previous study, we demonstrated the presence of a position-specific antigen (AV-1) in chick limb buds at an early developmental stage. Here, we reported the temporal and spatial expressions and the biochemical characterization of the AV-1 antigen. Indirect immunofluorescence staining and immunoblot analysis clearly showed that the AV-1 antigen is a glycoprotein that is localized on the plasma membrane and that it is expressed from stage 19 and highly expressed at stages 22-26 in some middle-distal to anterior-distal region of limb buds. In the wing bud, at stage 28, the AV-1 antigen was faintly detected in the restricted space between the precartilaginous regions of the radius and the ulna, and those of the metacarpals 2 and 3, but not those of the metacarpals 3 and 4. Such stage-specific and "position-specific" expressions of the AV-1 antigen in limb buds strongly suggest that the AV-1 antigen or cells containing it are involved in determination of the limb pattern formation.
authors.affiliation: National Institute of Neuroscience, Tokyo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Ohsugi
authors.forename: H
authors.initials: H
authors.lastname: Ide
authors.forename: T
authors.initials: T
authors.lastname: Momoi
mesh_terms: D000367:Age Factors; D000818:Animals; D000941:Antigens; D000954:Antigens, Surface / Q000032:analysis*; D030161:Avian Proteins; D015153:Blotting, Western; D002356:Cartilage / Q000196:embryology; D002462:Cell Membrane / Q000276:immunology; D002642:Chick Embryo; D005121:Extremities / Q000196:embryology*; D005455:Fluorescent Antibody Technique; D008562:Membrane Glycoproteins / Q000502:physiology*; D008970:Molecular Weight; D009024:Morphogenesis
chemical_list: C112157:AV-1 antigen, Gallus gallus; D000941:Antigens; D000954:Antigens, Surface; D030161:Avian Proteins; D008562:Membrane Glycoproteins