An effective mutagenic method in Azotobacter vinelandii.
The acridine-like compound ICR-191 is an effective mutagenic agent in Azotobacter vinelandii. Selectable mutants, such as those resistant to chlorate, can be isolated without post-mutagenic segregation. Non selectable mutants, such as those unable to metabolize different sugars, can be easily isolated after twelve generations of post-mutagenic segregation.
Luque
F
F
Departamento de Genética, Facultad de Biología, Universidad de Sevilla.
Santero
E
E
Medina
J R
JR
Tortolero
M
M
D000585:Aminacrine / Q000031:analogs & derivatives; D000609:Aminoacridines; D001395:Azotobacter / Q000235:genetics* / Q000302:isolation & purification; D001431:Bacteriological Techniques; D003470:Culture Media; D009153:Mutagens; D009154:Mutation; D009588:Nitrogen Mustard Compounds
D000609:Aminoacridines; D003470:Culture Media; D009153:Mutagens; D009588:Nitrogen Mustard Compounds; D000585:Aminacrine; C004294:acridine half-mustard