title: Ureaplasma urealyticum and male infertility: an animal model. I. Artificial infection, breeding experiments and histological preparation of organs.
abstract: Male Wistar rats were infected with Ureaplasma urealyticum, serotype 3 or 7, by injecting broth containing organisms into the urinary bladder following laparatomy. Animals were sacrificed 3, 7 and 21 days after infection. Ureaplasmas were detected in the organs of the genital tract by culture in 43% (serotype 3) and in 60% (serotype 7) of the animals. Type-specific ureaplasma antigen was detected in the genital organs in 44% (serotype 3) and 45% (serotype 7) of the animals. Control animals injected with sterile bouillon were negative for organisms and antigen. Male Wistar rats artificially infected with Ureaplasma urealyticum, serotype 3, were mated 3 and 6 months after infection with ureaplasma free female rats. The mating experiment revealed a smaller mean litter size and a lower birth weight in the offspring of infected males compared with the control animals, but no general influence on the fertility of infected animals.
authors.affiliation: Humboldt University, School of Medicine (Charité), Berlin, German Democratic Republic.
authors.forename: S
authors.initials: S
authors.lastname: Engel
authors.forename: R
authors.initials: R
authors.lastname: Bollmann
authors.forename: W
authors.initials: W
authors.lastname: Sokolowska-Köhler
authors.forename: H
authors.initials: H
authors.lastname: Audring
authors.forename: H
authors.initials: H
authors.lastname: Klug
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis; D001424:Bacterial Infections / Q000150:complications* / Q000382:microbiology; D004195:Disease Models, Animal; D005260:Female; D005455:Fluorescent Antibody Technique; D007248:Infertility, Male / Q000209:etiology*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D014509:Ureaplasma / Q000276:immunology / Q000302:isolation & purification*
chemical_list: D000942:Antigens, Bacterial