[The use of immobilized antibodies against fibronectin for isolation of macromolecular complexes of fibronectin with other secreted proteins from human fibroblasts].
Interaction of labelled proteins from human fibroblast culture and the antibodies to fibronectin, which were used as soluble and Sepharose-immobilized preparations, was studied. Electrophoretic mobilities of the proteins bound to antibodies were compared. Soluble antibodies bound only fibronectin, while immobilized antibodies bound, except for fibronectin, other low molecular proteins. After treatment of the cultural proteins with bacterial collagenase two main components, besides fibronectin, were eliminated, thus suggesting their collagen-like nature. Two dimers of fibronectin per one trimer of procollagen were present in the material bound to immobilized antibodies to fibronectin. Control studies demonstrated that the data obtained were not an artefact occurring due to sorption of proteins on Sepharose or to unspecific actions of antibodies.
Belkin
V M
VM
Dol'nikova
A E
AE
Volodarskaia
S M
SM
Mazurov
V I
VI
D000906:Antibodies; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D005347:Fibroblasts / Q000378:metabolism; D005353:Fibronectins / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D006801:Humans; D007163:Immunosorbent Techniques; D046911:Macromolecular Substances; D011347:Procollagen / Q000302:isolation & purification* / Q000378:metabolism
D000906:Antibodies; D005353:Fibronectins; D046911:Macromolecular Substances; D011347:Procollagen
Ispol'zovanie immobilizovannykh antitel k fibronektinu dlia vydeleniia makromolekuliarnykh kompleksov fibronektina s drugimi sekretiruemymi belkami fibroblastov cheloveka.