Inhibition of the metabolism of N-nitrosoacetoxymethylmethylamine in the rat by disulfiram.
The stability of N-nitrosoacetoxymethylmethylamine (NAMM) in rat serum in vitro, the half-life in blood in vivo and the exhalation rate of 14CO2 after the application of 14C-labelled NAMM were studied with and without disulfiram (DSF) pre-treatment. It was found that the metabolism of NAMM is inhibited by DSF in vitro as well as in vivo. The influence is thought to be due to inhibition of esterase activity, as shown by comparing the kinetics of the degradation of NAMM with those of p-nitrophenylacetate. In contrast to this result the exhalation of 14CO2 does not seem to be influenced by the esterase inhibiting effect of DSF.
Frank
N
N
Wiessler
M
M
D000818:Animals; D002245:Carbon Dioxide / Q000378:metabolism; D002250:Carbon Radioisotopes; D002273:Carcinogens / Q000378:metabolism*; D003864:Depression, Chemical; D004128:Dimethylnitrosamine / Q000031:analogs & derivatives* / Q000378:metabolism; D004221:Disulfiram / Q000494:pharmacology*; D004950:Esterases / Q000097:blood; D005978:Glutathione / Q000378:metabolism; D006207:Half-Life; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D002250:Carbon Radioisotopes; D002273:Carcinogens; D002245:Carbon Dioxide; C014659:methyl(acetoxymethyl)nitrosamine; D004950:Esterases; D005978:Glutathione; D004128:Dimethylnitrosamine; D004221:Disulfiram