PC12 cell aggregation and dopamine production on EHS-derived extracellular matrix.
PC12 cells on plastic grow as a single-layered lawn of cells which synthesize, store and secrete dopamine. In contrast, PC12 cells cultured on Englebreth-Holm-Swarm (EHS) tumor-derived extracellular matrix grow into multicellular aggregates. Matrix dissolution and cell migration appear to follow aggregate formation. PC12 cell plating efficiency is decreased on EHS-matrix but the doubling time of cells on EHS-matrix is comparable to plastic. Dopamine secretion and cellular content determined with a radioenzymatic assay as well as dopamine synthesis determined with cation-exchange chromatography are similar on a per cell basis in cultures of PC12 cells on plastic and EHS-matrix.
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Division of Reproductive Biology and Behavior, Oregon Regional Primate Research Center, Beaverton 97006.
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D000818:Animals; D002449:Cell Aggregation; D002460:Cell Line; D004298:Dopamine / Q000096:biosynthesis*; D005109:Extracellular Matrix; D008855:Microscopy, Electron, Scanning; D008858:Microscopy, Phase-Contrast; D010673:Pheochromocytoma / Q000473:pathology; D012513:Sarcoma, Experimental / Q000648:ultrastructure*; D014407:Tumor Cells, Cultured / Q000473:pathology*
D004298:Dopamine