Analysis by fast protein liquid chromatography of variants of kappa-casein and their relevance to micellar structure and renneting.
Fast protein liquid chromatography was used to study the kappa-casein fraction of casein micelles from bulk milk and from milk from individual animals homozygous for kappa-caseins A and B. The extent of glycosylation of the kappa-casein appeared to have no effect on its distribution in casein micelles of different sizes, nor did it affect the rate at which kappa-casein was destroyed during renneting. The rate of breakdown of kappa-casein during renneting was also almost independent of micellar size. The results may indicate a difference between methods which analyse for intact kappa-casein or for the product macropeptide during renneting.
Dalgleish
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D000818:Animals; D002364:Caseins / Q000235:genetics* / Q000302:isolation & purification; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D012085:Chymosin; D005260:Female; D014644:Genetic Variation; D006720:Homozygote; D008823:Micelles; D008892:Milk / Q000032:analysis
D002364:Caseins; D008823:Micelles; C025350:rennet; D012085:Chymosin