Expression of hamster MHC class I antigens in transformed cells and tumours induced by human adenoviruses.
The steady-state levels of hamster MHC class I mRNA and cell surface protein were analysed in cells transformed by either adenovirus type 12 (Ad12) or types 2 or 5 (Ad2 or Ad5). All cell lines were oncogenic in new born and adult hamsters. A great reduction in both class I mRNA and protein was observed in Ad12 transformed cells compared to cells transformed by Ad2 or Ad5. Analysis of class I mRNA in solid tumours induced in hamsters by Ad transformed cell lines also showed greatly reduced mRNA levels in tumours induced by Ad12 compared to those induced by Ad2 or Ad5. This suggests that, in the Ad transformed hamster cell system, reduction in the level of MHC class I gene expression is not necessarily associated with tumour formation.
Ackrill
A M
AM
Department of Biochemistry, University of Leeds, U.K.
Blair
G E
GE
D000260:Adenoviruses, Human; D000818:Animals; D015152:Blotting, Northern; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D006224:Cricetinae; D015342:DNA Probes; D005805:Genes, MHC Class I; D015395:Histocompatibility Antigens Class I / Q000096:biosynthesis*; D008565:Membrane Proteins / Q000032:analysis; D008647:Mesocricetus; D008970:Molecular Weight; D009374:Neoplasms, Experimental / Q000235:genetics / Q000276:immunology*; D012333:RNA, Messenger / Q000032:analysis; D012334:RNA, Neoplasm / Q000032:analysis; D001613:beta 2-Microglobulin / Q000032:analysis
D015342:DNA Probes; D015395:Histocompatibility Antigens Class I; D008565:Membrane Proteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D001613:beta 2-Microglobulin