Evolution of allantoinase and allantoicase involved in urate degradation in liver peroxisomes. A rapid purification of amphibian allantoinase and allantoicase complex, its subunit locations of the two enzymes, and its comparison with fish allantoinase and allantoicase.
Allantoinase and allantoicase are located in the same protein molecule in amphibian liver, whereas the two enzymes are different proteins in marine fish and invertebrate liver (Takada, Y., and Noguchi, T. (1983) J. Biol. Chem. 258, 4762-4764). The amphibian enzyme was rapidly purified from frog liver by using its following characteristics. 1) The enzyme binds to the intracellular membranes in the hypotonic solution. 2) The membrane-bound enzyme is not solubilized by the detergent. 3) The membrane-bound enzyme is solubilized by oxaloacetate. The electrophoresis of the purified enzyme gave a single protein band in the absence of sodium dodecyl sulfate, and gave two protein bands with molecular weights of 48,000 and 54,000, respectively, in the presence of sodium dodecyl sulfate. With a specific antibody raised against each subunit, allantoinase activity was found to be from the large subunit, and allantoicase activity to be from the small subunit. This amphibian allantoinase and allantoicase complex was compared with allantoinase and allantoicase purified from fish liver. Fish allantoinase was a single peptide and fish allantoicase was composed of two identical subunits. Fish allantoinase had an identical molecular weight with amphibian large (allantoinase) subunit and the subunit of fish allantoicase with amphibian small (allantoicase) subunit. These results suggest that the evolution of fish to amphibian resulted in the dissociation of allantoicase into subunits and in the association of allantoinase with allantoicase. The two enzymes are lost by further evolution.
Noguchi
T
T
Fujiwara
S
S
Hayashi
S
S
D000581:Amidohydrolases / Q000302:isolation & purification*; D000818:Animals; D001001:Anura; D004591:Electrophoresis, Polyacrylamide Gel; D005399:Fishes; D005779:Immunodiffusion; D008099:Liver / Q000648:ultrastructure*; D046911:Macromolecular Substances; D008830:Microbodies / Q000201:enzymology*; D009097:Multienzyme Complexes / Q000302:isolation & purification*; D014512:Ureohydrolases / Q000302:isolation & purification*
D046911:Macromolecular Substances; D009097:Multienzyme Complexes; D000581:Amidohydrolases; C019659:allantoinase; D014512:Ureohydrolases; C019658:allantoicase