Cell electrophoresis of group B streptococci: separation of types Ia, Ib/c, II, III and IV before and after neuraminidase treatment.
Group B streptococcal strains of types Ia, Ib/c, II, III and IV could be characterized by distinct electrophoretic mobilities before and after neuraminidase treatment. By this method of cell electrophoresis in the type I a strain, two subpopulations could be detected; whereas in all strains the electrophoretic mobility is markedly reduced after enzymatic removal of neuraminic acid, type II remained unaffected. The method of "bacteriopheresis" offers a new approach to classification of bacteria with respect to the primary and secondary surface structures.
Uhlenbruck
G
G
Institute for Immunobiology, University Hospital, Cologne.
Fröml
A
A
Lütticken
R
R
Hannig
K
K
D004586:Electrophoresis; D009438:Neuraminic Acids / Q000378:metabolism; D009439:Neuraminidase / Q000378:metabolism*; D011135:Polysaccharides, Bacterial / Q000032:analysis*; D013292:Streptococcus agalactiae / Q000032:analysis / Q000145:classification*
D009438:Neuraminic Acids; D011135:Polysaccharides, Bacterial; D009439:Neuraminidase