title: Structural characterization of a chain termination mutant of human serum albumin.
abstract: Mutant forms of human serum albumin have been detected on the basis of their abnormal electrophoretic mobility which is either faster or slower than that of normal albumin. In the present work we have studied the structure of a slow variant, referred to as albumin Ge/Ct, in order to define the cause of its genetic abnormality. The protein was isolated from the serum of a young healthy woman homozygous for the variant. Analysis of CNBr fragments by isoelectric focusing allowed us to localize the mutation to the COOH-terminal region of the molecule (residues 549-585). This fragment was isolated on a preparative scale and subjected to tryptic digestion. All tryptic peptides were purified by reverse-phase high performance liquid chromatography and characterized. Sequential analysis of three abnormal peptides revealed that albumin Ge/Ct has a shortened chain with the following COOH-terminal sequence: Leu-Val-Ala-Ala-Ser-Lys580-Leu-Pro. The presence of an additional lysine residue accounts for the electrophoretic behavior of the variant. It is likely that the variant may be caused by a single base deletion in the structural gene, a Cyt in mRNA codon 580, and the consequent shift in reading frame.
authors.forename: M
authors.initials: M
authors.lastname: Galliano
authors.forename: L
authors.initials: L
authors.lastname: Minchiotti
authors.forename: P
authors.initials: P
authors.lastname: Iadarola
authors.forename: M C
authors.initials: MC
authors.lastname: Zapponi
authors.forename: G
authors.initials: G
authors.lastname: Ferri
authors.forename: A A
authors.initials: AA
authors.lastname: Castellani
mesh_terms: D000328:Adult; D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D003488:Cyanogen Bromide; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006720:Homozygote; D006801:Humans; D007525:Isoelectric Focusing; D008239:Lysine / Q000032:analysis; D009154:Mutation; D012709:Serum Albumin / Q000235:genetics*; D013329:Structure-Activity Relationship; D014357:Trypsin / Q000378:metabolism
chemical_list: D012709:Serum Albumin; D014357:Trypsin; D008239:Lysine; D003488:Cyanogen Bromide