title: An ELISA test for detecting chloroquine in urine.
abstract: Two ELISA tests for detecting chloroquine in urine have been developed using polyclonal and monoclonal antibodies which react with the 7-chloro-4-amino-quinoline part of the chloroquine molecule and thus recognize chloroquine, its metabolites, and amodiaquine. The ELISAs were sensitive and specific and did not cross-react with other commonly used antimalarials. In a field trial the chloroquine ELISA performed better than the Dill Glazko or Haskins colorimetric tests. A small proportion of urines gave an apparently false positive reaction when tested at a dilution of 1:10, but not when tested at higher dilution.
authors.affiliation: Medical Research Council Laboratories, Fajara, Banjul, The Gambia.
authors.forename: F C
authors.initials: FC
authors.lastname: Shenton
authors.forename: M
authors.initials: M
authors.lastname: Bots
authors.forename: A
authors.initials: A
authors.lastname: Menon
authors.forename: T A
authors.initials: TA
authors.lastname: Eggelte
authors.forename: M
authors.initials: M
authors.lastname: de Wit
authors.forename: B M
authors.initials: BM
authors.lastname: Greenwood
mesh_terms: D000634:Aminoquinolines / Q000032:analysis; D000911:Antibodies, Monoclonal; D002675:Child, Preschool; D002738:Chloroquine / Q000652:urine*; D003124:Colorimetry; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D012680:Sensitivity and Specificity
chemical_list: D000634:Aminoquinolines; D000911:Antibodies, Monoclonal; C047186:7-chloro-4-aminoquinoline; D002738:Chloroquine