title: Failure of PAF-acether to induce in vivo neutrophil migration.
abstract: The ability of PAF-acether (platelet activating factor, 1-alkyl-2-acetyl-sn-glycero-3-phosphocholine) to induce neutrophil migration was tested in the peritoneal cavities of rats and guinea-pigs and in rat skin air pouches. PAF-acether, at doses of 2 and 5 micrograms per animal, did not promote significant neutrophil migration. The ability of PAF-acether antagonists to inhibit carrageenin induced neutrophil migration in rat abdominal cavities was also investigated. Carrageenin is a substance described to cause the release of endogenous PAF-acether. Neutrophil migration was not affected by PAF-antagonists. These observations challenge the possible role of PAF-acether as a neutrophil chemotactic factor in vivo.
authors.affiliation: Department of Pharmacology, Faculty of Medicine, University of São Paulo, Ribeirão Preto, Brazil.
authors.forename: F Q
authors.initials: FQ
authors.lastname: Cunha
authors.forename: N E
authors.initials: NE
authors.lastname: Heluy-Neto
authors.forename: A
authors.initials: A
authors.lastname: Klein
authors.forename: G E
authors.initials: GE
authors.lastname: Souza
authors.forename: S H
authors.initials: SH
authors.lastname: Ferreira
mesh_terms: D000818:Animals; D002351:Carrageenan; D002630:Chemotactic Factors / Q000502:physiology; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D006168:Guinea Pigs; D016209:Interleukin-8; D008297:Male; D009504:Neutrophils / Q000187:drug effects* / Q000276:immunology / Q000502:physiology; D010972:Platelet Activating Factor / Q000494:pharmacology* / Q000502:physiology; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D002630:Chemotactic Factors; D016209:Interleukin-8; D010972:Platelet Activating Factor; D002351:Carrageenan