T cell receptor and immunoglobulin gene rearrangements in acute myeloblastic leukemia.
The organization and expression of the beta chain of T cell antigen receptor gene (beta-TCR) and Ig H and L chain genes were analyzed by Southern blot technique in 24 patients with a diagnosis of acute myeloblastic leukemia (AML). Rearrangements of the beta-TCR genes were seen in DNA samples from 3 of the 24 patients. One of these three patients also showed rearrangement of the Ig H chain gene. RNA samples from all three patients expressed a beta-TCR gene transcript on dot blot analysis. However, on Northern blot analysis, one patient expressed an incomplete 1.0 kb transcript and no Ig H chain mRNA, despite a rearranged configuration. The karyotypes of two of these patients showed abnormalities involving chromosome 7. Rearrangements of T cell antigen receptor genes may occur in nonlymphoid malignancy, and is consistent with the concept of lineage infidelity in AML.
Cheng
G Y
GY
Minden
M D
MD
Toyonaga
B
B
Mak
T W
TW
McCulloch
E A
EA
D000293:Adolescent; D000328:Adult; D000368:Aged; D002869:Chromosome Aberrations; D002872:Chromosome Deletion; D002906:Chromosomes, Human, 6-12 and X / Q000648:ultrastructure; D004247:DNA / Q000235:genetics; D004273:DNA, Neoplasm / Q000032:analysis*; D005260:Female; D005819:Genetic Markers; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D007147:Immunoglobulin Light Chains / Q000235:genetics*; D015470:Leukemia, Myeloid, Acute / Q000235:genetics* / Q000276:immunology; D008297:Male; D008875:Middle Aged; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*
D004273:DNA, Neoplasm; D005819:Genetic Markers; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D011948:Receptors, Antigen, T-Cell; D004247:DNA