title: Genetic characterization of the gene hupA encoding the HU-2 protein of Escherichia coli.
abstract: The gene hupA encoding the HU-2 (HU-alpha) protein of Escherichia coli was mapped between rpoBC at 90 min and metA at 90.5 min on the K-12 genome by plasmid integration and P1-mediated transduction studies. Genetic studies using plasmid rescue techniques and physical mapping demonstrated that the gene order is rpoBC-hupA-rrnE-metA. The rpoBC is located approximately 10 kb proximal to the hupA gene, and rrnE about 8 kb distal to it. Thus, the direction of hupA gene transcription is clockwise on the E. coli map [Bachmann B.J., Microbiol. Rev. 47 (1983) 180-230].
authors.affiliation: Laboratory of Molecular Genetics, Tsukuba Life Science Center, Institute of Physical and Chemical Research, Ibaraki, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Kano
authors.forename: M
authors.initials: M
authors.lastname: Wada
authors.forename: F
authors.initials: F
authors.lastname: Imamoto
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D005796:Genes; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D010957:Plasmids; D015183:Restriction Mapping; D014161:Transduction, Genetic
chemical_list: D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C040330:histone-like protein HU, bacteria