[Binding of RNA-polymerase from Escherichia coli with oligodeoxyribonucleotides homologous to transcribed and non-transcribed DNA stands in the "-10"-promoter region of bacterial genes].
By using synthetic oligodeoxynucleotides related to the "-10" regions of spc and lacUV5 E. coli promoters we have shown, that the efficient binding occurs with oligodeoxynucleotides non-transcribed DNA strands. The duplexes containing oligodeoxynucleotides from the non-transcribed and transcribed DNA strands are also bound effectively by RNA polymerase. Oligodeoxynucleotides related to the non-transcribed DNA strands can effectively inhibit the RNA synthesis catalyzed by E. coli RNA polymerase. The data obtained allowed to consider the possible mechanism of selective binding of oligodeoxynucleotides by E. coli RNA polymerase and inhibition of the transcription.
Savinkova
L K
LK
Baranova
L V
LV
Knorre
V L
VL
Salganik
R I
RI
D001483:Base Sequence; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
D009838:Oligodeoxyribonucleotides; D012321:DNA-Directed RNA Polymerases
Sviazyvanie RNK-polimerazy Escherichia coli s oligodezoksiribonukleotidami, identichnymi transkribiruemoĭ i netranskribiruemoĭ nitiam DNK "-10"-oblasti promotorov bakterial'nykh genov.