title: Insulin modulation of pyruvate dehydrogenase in human circulating lymphocytes.
abstract: 1. In human circulating lymphocytes pyruvate dehydrogenase (PDH) complex is present in the active (PDHa) and inactive (PDHi) forms. 2. PDHi conversion into PDHa is stimulated when intact lymphocytes are incubated with 5 microU/ml insulin at pH 7.4, for 15 min at 37 degrees C in a medium supplemented with 50 microM Ca2+-Mg2+. 3. The generation of a mediator is strongly suggestive since a cell free preparation from circulating lymphocytes, treated as above described, still stimulates PDHi----PDHa conversion, when combined with either disrupted or intact lymphocytes.
authors.affiliation: Dipartimento di Medicina ed Oncologia Sperimentale, Università di Torino, Italia.
authors.forename: M
authors.initials: M
authors.lastname: Curto
authors.forename: M
authors.initials: M
authors.lastname: Piccinini
authors.forename: R
authors.initials: R
authors.lastname: Bruno
authors.forename: M
authors.initials: M
authors.lastname: Mostert
authors.forename: M T
authors.initials: MT
authors.lastname: Rinaudo
mesh_terms: D002118:Calcium / Q000494:pharmacology; D002474:Cell-Free System; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D007328:Insulin / Q000494:pharmacology*; D008214:Lymphocytes / Q000201:enzymology*; D008274:Magnesium / Q000494:pharmacology; D011768:Pyruvate Dehydrogenase Complex / Q000097:blood*
chemical_list: D007328:Insulin; D011768:Pyruvate Dehydrogenase Complex; D008274:Magnesium; D002118:Calcium