Effect of DL-3-hydroxybutyrate infusions on leucine and glucose kinetics in burned rats receiving TPN.
Whole-body leucine and plasma glucose kinetics were simultaneously measured in burned rats after 2 d of total parenteral nutrition (TPN) containing sodium DL-3-hydroxybutyrate or sodium acetate to evaluate the ketone bodies as energy substrates during stress. TPN solutions consisted of dextrose and amino acids [200 kcal/(kg . d); 13 g amino acids/(kg . d)] and contained 34.3 mEq/(kg . d) either as sodium DL-3-hydroxybutyrate (n = 8) or sodium acetate (n = 7). Whole-body leucine appearance, incorporation into protein, release from protein breakdown and oxidation rates, as measured after a constant infusion of L-[1-14C]leucine did not significantly differ between the groups. In contrast, D-[6-3H]glucose appearance rates after constant infusion of this tracer were significantly higher in rats given sodium DL-3-hydroxybutyrate [209.3 +/- 3.8 mumol/(kg body weight . min)] than in those given sodium acetate [162.4 +/- 9.7 mumol/(kg body weight . min)] (P less than 0.01). Since leucine kinetics did not differ, the results suggest that sodium DL-3-hydroxybutyrate infusions increase endogenous glucose production [61.0 +/- 4.6 mumol/(100 kg body weight . min)] by enhancing glucose recycling. However, there was no unique protein-sparing effect of ketone bodies identified during injury.
Maiz
A
A
Moldawer
L L
LL
Bistrian
B R
BR
Yamazaki
K
K
Mok
K T
KT
Blackburn
G L
GL
D020155:3-Hydroxybutyric Acid; D000596:Amino Acids / Q000097:blood; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism*; D002056:Burns / Q000378:metabolism* / Q000628:therapy; D006885:Hydroxybutyrates / Q000494:pharmacology*; D007263:Infusions, Parenteral; D007657:Ketone Bodies / Q000378:metabolism; D007700:Kinetics; D007930:Leucine / Q000097:blood*; D008099:Liver / Q000378:metabolism; D008297:Male; D009132:Muscles / Q000378:metabolism; D010101:Oxygen Consumption; D010289:Parenteral Nutrition, Total; D051381:Rats; D011919:Rats, Inbred Strains; D013312:Stress, Physiological / Q000378:metabolism
D000596:Amino Acids; D001786:Blood Glucose; D006885:Hydroxybutyrates; D007657:Ketone Bodies; D007930:Leucine; D020155:3-Hydroxybutyric Acid