title: [Interaction of muscle glycogen phosphorylase B with F-actin].
abstract: The binding of rabbit muscle glycogen phosphorylase b to F-actin has been studied by sedimentation in analytical centrifuge in 10 mM Tris-acetate buffer pH 6.8 at 20 degrees C. The adsorption capacity of F-actin is equal to (7.8 +/- 0.9) X 10(-7) mole of glycogen phosphorylase b per 1 g of F-actin; the microscopic dissociation constant for the glycogen phosphorylase-F-actin complex is (5.4 +/- 0.5) X 10(-7) M. It was found that the allosteric activator, AMP, facilitates the adsorption of glycogen phosphorylase b on F-actin, whereas the substrate, Pi, and the inhibitor, ATP, cause an opposite effect.
authors.forename: N A
authors.initials: NA
authors.lastname: Chebotareva
authors.forename: N P
authors.initials: NP
authors.lastname: Sugrobova
authors.forename: M V
authors.initials: MV
authors.lastname: Ostrovskaia
authors.forename: N B
authors.initials: NB
authors.lastname: Livanova
authors.forename: B F
authors.initials: BF
authors.lastname: Poglazov
mesh_terms: D000199:Actins / Q000378:metabolism*; D000327:Adsorption; D000818:Animals; D001665:Binding Sites; D066298:In Vitro Techniques; D007700:Kinetics; D009132:Muscles / Q000201:enzymology*; D010763:Phosphorylase b / Q000378:metabolism*; D006005:Phosphorylases / Q000378:metabolism*; D011817:Rabbits; D013379:Substrate Specificity
chemical_list: D000199:Actins; D010763:Phosphorylase b; D006005:Phosphorylases
vernacular_title: Vzaimodeĭstvie myshechnoĭ glikogenfosforilay B s F-aktinom.