[Comparison of thawing of plasma by microwave or water bath: preliminary longitudinal biological study of hemostatic parameters].
Exploration of haemostasis was performed on plasmas thawed in an experimental microwave oven comparatively to a 37 degrees C water bath. Factor VIII:R:Ag, procoagulant and antigenic fibrinogen, and Fg:C/Fg:Ag ratio were found to be significantly, slightly decreased with microwave thawing. Factor VIII:C and VIII:C/VIII:R:Ag ratio were found to be increased with microwaves. Antigenic fractions were decreased because of partial precipitation. In addition, Fibrinogen slightly lost its activity; on the contrary, factor VIIIC was activated by micro-waves. All this allows to select parameters for new experimental microwave ovens development.
Gris
J C
JC
Centre de Transfusion Sanguine des Armées, Clamart.
Joussemet
M
M
Bourin
P
P
Fabre
G
G
Schved
J F
JF
D001779:Blood Coagulation Factors / Q000276:immunology; D001780:Blood Coagulation Tests; D001793:Blood Preservation / Q000379:methods*; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation; D006358:Hot Temperature; D006801:Humans; D007158:Immunologic Techniques; D066298:In Vitro Techniques; D008872:Microwaves; D013917:Thrombin / Q000032:analysis
D001779:Blood Coagulation Factors; D013917:Thrombin
Décongélation comparative du plasma par micro-ondes et bain-marie: étude biologique longitudinale préliminaire de paramètres de l'hémostase.