title: Effects of hypoxia and hyperoxia on proteoglycan production by bovine pulmonary artery endothelial cells.
abstract: Bovine pulmonary artery endothelial cells in culture were used to assess the influence of oxygen tension on proteoglycan synthesis. Cells exposed to 3% O2 (hypoxia) for 72 h and then labeled with [35S]sulfate for 5 h accumulated significantly less [35S]proteoglycan in medium than cells exposed to 20% O2 (control). This decrease was due primarily to a reduction in heparan sulfate. Cells exposed to 80% O2 (hyperoxia) for 72 h secreted slightly more [35S]proteoglycan into medium than controls. Greater accumulation of chondroitin sulfate was responsible for the increase. The amount of cell-associated proteoglycan did not change significantly in cells cultured in 3% or 80% O2 as compared with control cells cultured in 20% O2. Proteoglycans produced by hypoxia- or hyperoxia-treated cells were found to be similar in size to proteoglycans produced by cells cultured at 20% O2. Glycosaminoglycan sulfation, as measured by ion-exchange chromatography, did not appear to change with varying oxygen tensions. Our results demonstrate that production of proteoglycans secreted by endothelial cells in culture is sensitive to oxygen tension.
authors.forename: D E
authors.initials: DE
authors.lastname: Humphries
authors.forename: S L
authors.initials: SL
authors.lastname: Lee
authors.forename: B L
authors.initials: BL
authors.lastname: Fanburg
authors.forename: J E
authors.initials: JE
authors.lastname: Silbert
mesh_terms: D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D002809:Chondroitin Sulfates / Q000096:biosynthesis; D004727:Endothelium / Q000187:drug effects / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D006497:Heparan Sulfate / Q000096:biosynthesis; D010100:Oxygen / Q000494:pharmacology*; D011509:Proteoglycans / Q000096:biosynthesis*; D011651:Pulmonary Artery / Q000187:drug effects / Q000378:metabolism*
chemical_list: D002809:Chondroitin Sulfates; D006497:Heparan Sulfate; D010100:Oxygen; D011509:Proteoglycans