title: Application of HPLC with a metal-free column to the analysis of cyclosporin in whole blood samples from cardiac transplant patients.
abstract: A simplified, reverse-phase HPLC method utilizing a metal-free column was developed for the analysis of cyclosporin (CYA) in whole blood in humans. The average intra and inter assay variation at the low end of the therapeutic range are less than or equal to 4.5 and 8.8% respectively. The procedure was successfully applied to the measuring of CYA in whole blood collected from cardiac transplant patients over a one year period.
authors.affiliation: Bureau of Drug Research, Health Protection Branch, Ottawa, Ontario, Canada.
authors.forename: J C
authors.initials: JC
authors.lastname: Loo
authors.forename: N
authors.initials: N
authors.lastname: Jordan
authors.forename: I J
authors.initials: IJ
authors.lastname: McGilveray
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003524:Cyclosporins / Q000097:blood*; D016027:Heart Transplantation; D006801:Humans; D008670:Metals
chemical_list: D003524:Cyclosporins; D008670:Metals