[Mutagenicity of benzene metabolites by fluctuation test].
Mutagenicity of five benzene metabolites was examined by a fluctuation test modified by Gatehouse. The test was performed by using Salmonella typhimurium TA100 with or without a metabolic activation system. Catechol showed mutagenic activity with and without a metabolic activation system in the fluctuation test. Hydroxyhydroquinone, phenol, hydroquinone and p-benzoquinone were positive only with a metabolic activation system in this test. The Ames' plate method and preincubation method are widely used as a screening test for the mutagenic compounds. However, the fluctuation test is very useful in detecting mutagenic activity of chemicals which are negative in the Ames test though being a mutagen or carcinogen.
Koike
N
N
Department of Public Health, Jikei University School of Medicine.
Haga
S
S
Ubukata
N
N
Sakurai
M
M
Shimizu
H
H
Sato
A
A
D000818:Animals; D001554:Benzene / Q000378:metabolism*; D016227:Benzoquinones; D001711:Biotransformation; D002396:Catechols / Q000633:toxicity*; D006873:Hydroquinones / Q000633:toxicity*; D008297:Male; D009152:Mutagenicity Tests / Q000379:methods; D019800:Phenol; D010636:Phenols / Q000633:toxicity*; D011809:Quinones / Q000633:toxicity*; D051381:Rats; D011919:Rats, Inbred Strains; D012486:Salmonella typhimurium / Q000235:genetics
D016227:Benzoquinones; D002396:Catechols; D006873:Hydroquinones; D010636:Phenols; D011809:Quinones; C013038:hydroxyhydroquinone; D019800:Phenol; C004532:quinone; D001554:Benzene; C034221:catechol; C031927:hydroquinone