Functional probing of glucocorticoid receptor structure.
The glucocorticoid receptor (GR) consists of three functional domains. The steroid-binding domain is situated at the C-terminal end and N-terminal sequence analysis has identified the border of this domain at residue 518. The DNA-binding domain is central and lies between residues 414 and 517. The remaining N-terminal half of the protein constitutes the third domain. Probing of the steroid-receptor interaction by affinity-labelling and radiosequence analysis has identified three steroid-binding amino acid residues, Met-622, Cys-656 and Cys-754.
Carlstedt-Duke
J
J
Department of Medical Nutrition, Karolinska Institute, Huddinge University Hospital, Sweden.
Gustafsson
J A
JA
D000818:Animals; D001665:Binding Sites; D004268:DNA-Binding Proteins / Q000502:physiology; D005938:Glucocorticoids / Q000378:metabolism; D008970:Molecular Weight; D009687:Nuclear Proteins / Q000648:ultrastructure*; D010447:Peptide Hydrolases / Q000378:metabolism; D011965:Receptors, Glucocorticoid / Q000502:physiology / Q000648:ultrastructure*; D012045:Regulatory Sequences, Nucleic Acid
D004268:DNA-Binding Proteins; D005938:Glucocorticoids; D009687:Nuclear Proteins; D011965:Receptors, Glucocorticoid; D010447:Peptide Hydrolases