Packaging of transducing DNA by bacteriophage P1.
P1 transduces bacterial chromosomal markers with widely differing frequencies. We use quantitative Southern hybridisations here to show that, despite this, most markers are packaged at similar levels. Exceptions are a group of markers near 2 min and another at 90 min which seem to be packaged at levels two- to threefold higher. We thus conclude that certain marker frequency variations in transduction can be explained by differences in packaging level, but that most cannot. The limited range in packaging levels suggests that P1 can initiate the packaging of chromosomal DNA from many sites. This idea is supported by our failure to find any chromosomal sequences with homology to the phage pac site and by the occurrence of hybridising bands which seem to suggest sequential packaging from a large number of specific sites. We eliminate the possibility that chromosomal DNA packaging is the result of endonucleolytic cutting by the P1 res enzyme.
Hanks
M C
MC
Department of Molecular Biology, King's Buildings, Edinburgh, Scotland.
Newman
B
B
Oliver
I R
IR
Masters
M
M
D001345:Autoradiography; D001435:Bacteriophages / Q000235:genetics* / Q000502:physiology; D002213:Capsid / Q000502:physiology*; D015342:DNA Probes; D004269:DNA, Bacterial / Q000032:analysis; D004279:DNA, Viral / Q000032:analysis / Q000502:physiology*; D004926:Escherichia coli / Q000032:analysis / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D014161:Transduction, Genetic
D015342:DNA Probes; D004269:DNA, Bacterial; D004279:DNA, Viral