Antiserum to endotoxin in hemorrhagic shock.
Antiserum to Escherichia coli J5, a mutant endotoxin (LPS) which contains only core determinants, has proven effective in reducing mortality from endotoxic shock due to a wide variety of gram-negative bacteria. Twenty New Zealand white rabbits with coliforms in the gut were subjected to hemorrhagic shock of 36 mm Hg for 3 hr. Treated rabbits were resuscitated with 15 cc of rabbit J5 antiserum (hemagglutinating antibody titer against J5 lipopolysaccharide of 1:1024), remaining shed blood, and lactated Ringer's to achieve a mean arterial blood pressure (MABP) within 20% of baseline. The control group was similarly resuscitated but received 15 cc normal rabbit serum (titer 1:2). Catheters were removed and rabbits were returned to their cages until death or 5 days of survival. Hemodynamic parameters (heart rate, MABP, cardiac output, and total peripheral resistance) did not differ significantly between groups. However, six treated rabbits survived 5 days (60%) and no control rabbit lived past the third postexperimental day (P less than 0.019). Our data suggest that systemic endotoxemia may contribute to morbidity and mortality in severe hemorrhagic shock.
Pohlson
E C
EC
University of Hawaii School of Medicine, Queen's Medical Center, Honolulu 96813.
Suehiro
A
A
Ziegler
E J
EJ
Suehiro
G
G
McNamara
J J
JJ
D000410:Alanine Transaminase / Q000378:metabolism; D000818:Animals; D004731:Endotoxins / Q000276:immunology*; D004926:Escherichia coli / Q000276:immunology*; D007106:Immune Sera / Q000276:immunology*; D007167:Immunotherapy; D011817:Rabbits; D012771:Shock, Hemorrhagic / Q000201:enzymology / Q000401:mortality / Q000628:therapy*; D013997:Time Factors
D004731:Endotoxins; D007106:Immune Sera; D000410:Alanine Transaminase