Studies on follicular atresia: role of tropic hormone and steroids in regulating cathepsin-D activity of preantral follicles of the immature rat.
The ovary of the immature female rat is comprised of primary and medium-sized preantral follicles. Upon stimulation with FSH or PMSG, the cathepsin-D activity, a representative lysosomal enzyme of granulosa cells, is reduced by 50% (P less than 0.01). 17 beta-Estradiol at the doses tried was unable to mimic this effect. Blockade of steroidogenesis with cyanoketone also had no effect on the cathepsin-D activity of isolated granulosa cells. Dihydrotestosterone (DHT), however, at a dose of 1 mg/rat was able to inhibit PMSG's tropic action. It brought about an increase in cathepsin-D activity and reduction in steroidogenic activity of isolated granulosa cells. The atretogenic activity of DHT could be relieved by supplementation with exogenous FSH. DHT was observed to significantly reduce (P less than 0.01) endogenous FSH and LH levels within 12-18 h of its injection suggesting that its atretic effect was due to its action at the pituitary rather than the gonad. In addition to the above the ability of 15 IU of PMSG to reduce cathepsin-D activity of granulosa cells was also significantly reduced (P less than 0.01) if endogenous FSH was neutralized by a specific FSH antiserum. The present study suggests that as far as small and medium-sized primary and preantral follicles are concerned, FSH lack is the essential signal for onset of atresia.
Dhanasekaran
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N
Moudgal
N R
NR
D000305:Adrenal Cortex Hormones / Q000494:pharmacology*; D000818:Animals; D002402:Cathepsin D / Q000378:metabolism*; D003489:Cyanoketone / Q000494:pharmacology; D013196:Dihydrotestosterone / Q000494:pharmacology; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D005640:Follicle Stimulating Hormone / Q000172:deficiency / Q000378:metabolism; D005496:Follicular Atresia; D005498:Follicular Phase; D006062:Gonadotropins / Q000502:physiology*; D006064:Gonadotropins, Equine / Q000378:metabolism; D006107:Granulosa Cells / Q000201:enzymology*; D007106:Immune Sera / Q000494:pharmacology; D007986:Luteinizing Hormone / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012741:Sexual Maturation; D013256:Steroids / Q000502:physiology*
D000305:Adrenal Cortex Hormones; D006062:Gonadotropins; D006064:Gonadotropins, Equine; D007106:Immune Sera; D013256:Steroids; D013196:Dihydrotestosterone; D003489:Cyanoketone; D004958:Estradiol; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D002402:Cathepsin D