title: Dot-ELISA, a potential immunoassay for the detection of Plasmodium falciparum antibodies.
abstract: The dot enzyme-linked immunosorbent assay (dot-ELISA) was compared to the indirect immunofluorescent assay (IIFA) and radioimmunoassay (RIA) for the detection of malaria antibodies. Of 281 sera tested by dot-ELISA, 220 were from Ethiopia, 11 from the Sudan, and 50 from Egypt. A close correlation between the dot-ELISA and RIA results was observed in 92% of the 220 Ethiopian cases. Of the remainder, 6% gave positive RIA and negative dot-ELISA results, and 2% gave positive dot-ELISA and negative RIA results. Antibody titres determined by dot-ELISA and RIA were positively correlated in 10 of the 11 Sudanese cases tested by direct microscopical examination. The eleventh case was positive by dot-ELISA at 1:1000 dilution, but negative by RIA and direct examination. With the 50 Egyptian sera, the dot-ELISA results showed close correspondence to the IIFA results, but the dot-ELISA was 20-40 fold more sensitive than the IIFA. To test specificity, 62 samples from patients with 11 different diseases and conditions were examined by dot-ELISA. No malaria antibodies were detected in any of these or in sera from healthy controls. Dot-ELISA is a potentially useful method for sero-epidemiological studies of malaria.
authors.affiliation: Kuvin Centre for the Study of Infectious and Tropical Diseases, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
authors.forename: M V
authors.initials: MV
authors.lastname: Londner
authors.forename: A
authors.initials: A
authors.lastname: Revel
authors.forename: G
authors.initials: G
authors.lastname: Rosen
authors.forename: M G
authors.initials: MG
authors.lastname: Shehata
authors.forename: M A
authors.initials: MA
authors.lastname: Kemawi
authors.forename: S
authors.initials: S
authors.lastname: el Said
authors.forename: M
authors.initials: M
authors.lastname: Said
authors.forename: T
authors.initials: T
authors.lastname: Spira
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000032:analysis*; D000918:Antibody Specificity; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006801:Humans; D008288:Malaria / Q000175:diagnosis; D010963:Plasmodium falciparum / Q000276:immunology*; D011863:Radioimmunoassay
chemical_list: D000913:Antibodies, Protozoan