title: Influence of nicotine on monocyte phenotype and function.
abstract: Monocyte expression of class II antigens was studied in smokers and in nonsmokers using double marker analysis and flow cytometry. There was no difference in class II expression with 78.8% and 79.3% class II+ monocytes in smokers and nonsmokers, respectively. Further, in vitro treatment with nicotine at levels seen in smokers (50 ng/ml) did not alter class II expression either. When analyzing TNF production using the Wehi 164/ActD 51Cr-release assay we observed a two-fold lower TNF production in smokers versus nonsmokers. In vitro treatment of monocytes with nicotine, however, did not reduce the LPS triggered TNF production.
authors.affiliation: Institut für Immunologie, Universität München.
authors.forename: H W
authors.initials: HW
authors.lastname: Ziegler-Heitbrock
authors.forename: C
authors.initials: C
authors.lastname: Thiel
authors.forename: G
authors.initials: G
authors.lastname: Riethmüller
mesh_terms: D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation / Q000187:drug effects*; D006681:HLA-D Antigens / Q000235:genetics*; D006801:Humans; D009000:Monocytes / Q000187:drug effects*; D009538:Nicotine / Q000494:pharmacology*; D010641:Phenotype; D012907:Smoking / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
chemical_list: D006681:HLA-D Antigens; D014409:Tumor Necrosis Factor-alpha; D009538:Nicotine