title: Antigenic peptides on the surface of Dirofilaria immitis microfilariae.
abstract: Various methods of radioiodination were employed to identify peptides on the surface of Dirofilaria immitis microfilariae. Optimum surface radiolabelling occurred with the lactoperoxidase-catalyzed reaction. Two major peptides of 16 and 14 kDa were labelled by this method. These peptides were soluble in Nonidet P-40, were not glycosylated, and showed no signs of disulfide linkages. These peptides were immunoprecipitated by sera from D. immitis-infected dogs, but not by sera from uninfected dogs or sera from dogs with potentially cross-reactive nematode infections. Analysis of the 14 and 16 kDa peptides by two-dimensional gel electrophoresis revealed that the 16 kDa peptide was a single unit with a pI of 5.25 whereas the 14 kDa band was composed of three individual peptides with pI values ranging from 5.6 to 6.1. Iodination by chloramine T resulted in the same panel of labelled peptides but suffered from poor efficiency of 125I incorporation. The viability of microfilariae labelled by the standard Bolton-Hunter method decreased by 50% following the reaction which resulted in the labelling of a variety of internal components.
authors.forename: W K
authors.initials: WK
authors.lastname: Tamashiro
authors.forename: J P
authors.initials: JP
authors.lastname: Ehrenberg
authors.forename: D A
authors.initials: DA
authors.lastname: Levy
authors.forename: A L
authors.initials: AL
authors.lastname: Scott
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis* / Q000276:immunology; D011232:Chemical Precipitation; D004183:Dirofilaria immitis / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D005370:Filarioidea / Q000276:immunology*; D006026:Glycoside Hydrolases / Q000378:metabolism; D037102:Lectins; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase; D008970:Molecular Weight; D011506:Proteins / Q000276:immunology
chemical_list: D000947:Antigens, Helminth; D037102:Lectins; D011506:Proteins; D006026:Glycoside Hydrolases; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase