[Mutations of resistance to 6-thioguanine following roentgen irradiation and subsequent treatment with cytosine arabinoside combined with hydroxyurea in Chinese hamster cells in the G1 phase].
A study was made of the effect of two-hour treatment of Chinese hamster cells with cytosine arabinoside (AraC) combined with hydroxyurea (HU) at the G1 phase of the cell cycle on lethal and mutagenic effects of X-radiation (50 to 400 cGy). The inhibitors were shown to increase a spontaneous mutation level of the resistance to 6-thioguanine: this increase augmented by 3 times as the time the treatment increased from 1-2 to 6 h. However, while sharply enhancing the inactivating effect of X-radiation (the enhancement coefficient was 2.6) Arac+HU caused an additive, or a somewhat lesser, effect as estimated by the yield of mutations. It is suggested that AraC combined with hydroxyurea fail to modify the radiation-induced premutation damages.
Elisova
T V
TV
Feoktistova
T P
TP
Stavrakova
N M
NM
D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D006224:Cricetinae; D003412:Cricetulus; D003561:Cytarabine / Q000494:pharmacology*; D004351:Drug Resistance / Q000187:drug effects / Q000528:radiation effects; D006918:Hydroxyurea / Q000494:pharmacology*; D066298:In Vitro Techniques; D007399:Interphase; D009154:Mutation; D013866:Thioguanine / Q000494:pharmacology*
D003561:Cytarabine; D013866:Thioguanine; D006918:Hydroxyurea
Mutatsii rezistentnosti k 6-tioguaninu posle rentgenovskogo oblucheniia i posleduiushcheĭ obrabotki tsitozinarabinozidom v sochetanii s oksimochevinoĭ kletok kitaĭskogo khomiachka v G1 faze.