title: The specificity of nephritogenic antibodies. V. Glomerular localization of anti-GP 330 and anti-GP 90 antibodies present in passive Heymann serum.
abstract: Administration in the rat of rabbit antibodies directed against rat tubular brushborder antigens (FXIA) leads to membranous glomerulopathy (HICN) associated with glomerular immune complexes (ICXS). These anti-FXIA antibodies (Abs) contain two major specificities, anti-GP 330 and anti-GP 90. The contribution of each specificity to the localization of anti-FXIA Abs was studied by direct immunofluorescence. Perfusion studies, under conditions which avoid ischaemia, confirmed that in this system glomerular localization of anti-FXIA Abs depends only on anti-GP 90 Abs, and the failure of anti-GP 330 Abs to localize after perfusion could not be attributed to ischaemia. In contrast, intravenous (i.v.) injection of anti-GP 330 Abs results in granular deposits of rabbit IgG, similar to those observed using anti-FXIA Abs. Injection i.v. of anti-GP 90 Abs results only in (faint) linear deposits. Using alternating and combined perfusion studies with these Abs, it is shown that anti-GP 90 Abs do not influence localization of anti-GP 330 Abs. In addition, systemic administration of anti-GP 90 Abs combined with perfusion of anti-GP 330 Abs does not facilitate localization of anti-GP 330 Abs. Although directly after i.v. injection of anti-FXIA Abs some anti-GP 90 Abs probably localize in the glomerular capillary wall, our results exclude a dominant or ancillary role for anti-GP 90 Abs in the formation of glomerular Icxs in HICN.
authors.affiliation: Department of Pathology, University of Groningen, The Netherlands.
authors.forename: W M
authors.initials: WM
authors.lastname: Bagchus
authors.forename: P J
authors.initials: PJ
authors.lastname: Hoedemaeker
authors.forename: J F
authors.initials: JF
authors.lastname: Slegers
authors.forename: W W
authors.initials: WW
authors.lastname: Bakker
mesh_terms: D000818:Animals; D000918:Antibody Specificity; D001323:Autoantibodies / Q000008:administration & dosage / Q000032:analysis*; D005260:Female; D005455:Fluorescent Antibody Technique; D005921:Glomerulonephritis / Q000276:immunology*; D026541:Heymann Nephritis Antigenic Complex; D007275:Injections, Intravenous; D007678:Kidney Glomerulus / Q000276:immunology*; D008562:Membrane Glycoproteins / Q000276:immunology*; D010477:Perfusion; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D001323:Autoantibodies; D026541:Heymann Nephritis Antigenic Complex; D008562:Membrane Glycoproteins