Diagnosis of tuberculosis by detection of mycobacterial antigen in pleural effusions and ascites.
We describe a method for the diagnosis of pleural and peritoneal tuberculosis by the detection of tuberculous antigens using an enzyme-linked immunosorbent assay. Eleven tuberculous pleural fluid and 10 tuberculous ascitic fluid samples were studied by this technique, using 10 non-tuberculous pleural fluid and 14 non-tuberculous ascitic fluid samples as controls. An absorbance value of 0.3 was found to separate the tuberculous groups from their controls to a statistically significant extent (ascitic fluid P less than 0.05; pleural fluid P less than 0.01).
Baig
M M
MM
Pettengell
K E
KE
Simjee
A E
AE
Sathar
M A
MA
Vorster
B J
BJ
D000942:Antigens, Bacterial / Q000032:analysis*; D001201:Ascites / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D014395:Peritonitis, Tuberculous / Q000175:diagnosis*; D010996:Pleural Effusion / Q000276:immunology*; D014396:Tuberculosis, Pleural / Q000175:diagnosis*
D000942:Antigens, Bacterial