title: Total reconstitution of active large ribosomal subunits of the thermoacidophilic archaebacterium Sulfolobus solfataricus.
abstract: The large ribosomal subunit of the extremely thermoacidophilic archaebacterium Sulfolobus solfataricus has been reconstituted from the completely dissociated RNA and proteins by a two-step incubation procedure at high temperatures. Successful reconstitution requires a preliminary incubation of the ribosomal components for 45 min at 65 degrees C, followed by a second heat-treatment at 80 degrees C for 60 min. Structural reassembly depends upon high concentrations of K+ (300-400 mM) and Mg2+ (20-40 mM) ions. In addition, complete recovery of subunit function stringently requires the presence of a polyamine, thermine (or spermine). The reconstituted archaebacterial subunits are essentially indistinguishable from the native ones by a number of structural and functional criteria.
authors.forename: P
authors.initials: P
authors.lastname: Londei
authors.forename: J
authors.initials: J
authors.lastname: Teixidò
authors.forename: M
authors.initials: M
authors.lastname: Acca
authors.forename: P
authors.initials: P
authors.lastname: Cammarano
authors.forename: R
authors.initials: R
authors.lastname: Amils
mesh_terms: D001105:Archaea / Q000648:ultrastructure*; D001419:Bacteria / Q000648:ultrastructure*; D006358:Hot Temperature; D066298:In Vitro Techniques; D007700:Kinetics; D008274:Magnesium; D009024:Morphogenesis; D009691:Nucleic Acid Denaturation; D011073:Polyamines; D011188:Potassium; D014176:Protein Biosynthesis; D012270:Ribosomes / Q000378:metabolism / Q000648:ultrastructure*
chemical_list: D011073:Polyamines; D008274:Magnesium; D011188:Potassium