title: Altered phosphorylation of rat neuronal cytoskeletal proteins in acrylamide induced neuropathy.
abstract: The activity of protein kinase has been assayed in neurofilament preparations from spinal cords of rats treated with acrylamide. Animals received 50 mg/kg, i.p., of acrylamide per day for a total of 5 or 10 days; these doses produce mild and marked symptoms of neurological damage, respectively. Incorporation of phosphate into proteins was determined using [gamma-32P]ATP followed by SDS-PAGE. Total phosphorylation of neurofilament preparations was significantly increased only in the animals treated with the 500 mg/kg cumulative dose of acrylamide. Phosphorylation of the 200 and 155 kdalton subunits of the neurofilaments was increased by 20-40% in the acrylamide treated groups. The phosphorylation of the 70 kdalton neurofilament subunit was unchanged in the 250 mg/kg group and was significantly decreased in the 500 mg/kg group. Phosphorylation of other protein bands was not altered. These results suggest a mechanism by which acrylamide might produce axonal neurofilamentous accumulations.
authors.forename: R D
authors.initials: RD
authors.lastname: Howland
authors.forename: P
authors.initials: P
authors.lastname: Alli
mesh_terms: D020106:Acrylamide; D000178:Acrylamides / Q000494:pharmacology*; D000818:Animals; D003598:Cytoskeletal Proteins / Q000378:metabolism*; D007381:Intermediate Filament Proteins / Q000378:metabolism; D008297:Male; D009422:Nervous System Diseases / Q000139:chemically induced* / Q000378:metabolism; D016900:Neurofilament Proteins; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013116:Spinal Cord / Q000378:metabolism*
chemical_list: D000178:Acrylamides; D003598:Cytoskeletal Proteins; D007381:Intermediate Filament Proteins; D016900:Neurofilament Proteins; D020106:Acrylamide; D011494:Protein Kinases