Detection of folliculostatin: a sensitive and specific bioassay using the female rat.
To establish a sensitive and specific bioassay for the FSH-suppressing activity present in porcine follicular fluid (pFF), we examined the latency of pFF action when injected IV in the acutely ovariectomized (ovax) metestrous rat. By 2h post injection (5.5h after ovax), FSH was suppressed significantly in pFF vs. porcine serum-injected controls. LH was unaltered. In an experiment establishing a dose-response curve for pFF 4.5h after injection, 1.77 mg of pFF protein significantly suppressed FSH. The index of precision (-0.2188) and precision of slope (1.088) were well within acceptable limits for bioassays. We conclude that the ovax metestrous rat, injected 3.5h after surgery and sacrificed at 4.5 or 5.5h, is a sensitive and specific bioassay for folliculostatin.
Savoy-Moore
R T
RT
Grady
R R
RR
Schwartz
N B
NB
D000818:Animals; D001681:Biological Assay; D001826:Body Fluids / Q000032:analysis; D005260:Female; D005640:Follicle Stimulating Hormone / Q000037:antagonists & inhibitors / Q000097:blood; D007265:Inhibins / Q000032:analysis* / Q000494:pharmacology; D007986:Luteinizing Hormone / Q000097:blood; D008686:Metestrus; D006080:Ovarian Follicle / Q000502:physiology*; D010052:Ovariectomy; D051381:Rats; D011919:Rats, Inbred Strains; D013552:Swine
D007265:Inhibins; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone