title: Junctional communication and cellular differentiation.
abstract: Gap junctions provide pathways of direct cell to cell communication in the tissues of metazoan animals. Cells joined by gap junctions share their small ions and molecules but can maintain distinctive activities through expression of different macromolecules which are too large to pass through the junctions. The junctional channels are made of a tissue invariant, evolutionarily conserved 16-18 k protein but the formation and maintenance of active coupling also requires one or more connexins, a family of tissue-specific proteins ranging in size from 21 k to 70 k. Junctions can be isolated as complexes containing both types of protein by mild procedures using high pH but the connexins can be removed by detergent, urea and protease treatment without destroying the characteristic junctional-morphology of hexagonally packed channels in the double membrane structures. There is also some evidence for the participation in the complex of tissue-specific proteoglycans which perhaps interact with the tissue-specific connexins and account for specificity of junction formation. Such specificity in mixed cultures leads to the production of communication compartments, groups of cells joined by junctions but separated by reduced trans-boundary coupling from cells in adjacent compartments. Compartmentation also occurs in vivo resulting in specific patterns of junctional communication which have been mapped in most detail in mouse skin. These mapping data and the changes which are associated with abnormal proliferation have lead to new ideas on intercellular control.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Beatson Institute for Cancer Research, Bearsden, Glasgow, Scotland, UK.
authors.forename: J D
authors.initials: JD
authors.lastname: Pitts
authors.forename: M E
authors.initials: ME
authors.lastname: Finbow
authors.forename: E
authors.initials: E
authors.lastname: Kam
mesh_terms: D000818:Animals; D001181:Arthropods; D002450:Cell Communication; D002451:Cell Compartmentation; D002454:Cell Differentiation; D002455:Cell Division; D007365:Intercellular Junctions / Q000502:physiology* / Q000648:ultrastructure; D014714:Vertebrates; D014961:X-Ray Diffraction