An enzyme immunoassay for the quantitation of rat liver carbamoyl-phosphate synthetase I.
An indirect, competitive enzyme-linked immunosorbent assay for the quantitation of carbamoyl-phosphate synthetase I (ammonia) in rat liver has been developed. Homogenization of the liver in 1% sodium deoxycholate is used for complete solubilization of the enzyme. The detergent does not interfere with the method if diluted to a concentration of 0.01% or lower. The assay is applied to determine the amount of enzyme in control rats and in rats fed "cafeteria" or high-protein diets. Changes in the amount of carbamoyl-phosphate synthetase I (ammonia) paralleled changes in enzymatic activity.
Cerda
M
M
Departamento de Bioquímica y Biologia Molecular, Facultad de Farmacia, Universidad de Valencia, Spain.
Jorda
A
A
Barber
T
T
Castell
J V
JV
Cabo
J
J
Timoneda
J
J
D000818:Animals; D002222:Carbamoyl-Phosphate Synthase (Ammonia) / Q000032:analysis*; D004032:Diet; D007124:Immunoenzyme Techniques / Q000379:methods*; D008099:Liver / Q000201:enzymology*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D002222:Carbamoyl-Phosphate Synthase (Ammonia)