Isolation and characterization of human platelet glycoprotein IX.
Human platelet glycoprotein IX is a small (Mr 17,000) surface glycoprotein present in the plasma membrane as a 1:1 non-covalent complex with glycoprotein Ib (Mr 165,000). Glycoprotein IX was purified to near homogeneity by sequential wheat germ agglutinin and immuno-affinity chromatography, followed by gel filtration chromatography under denaturing conditions. Purified glycoprotein IX was characterized by determination of its amino acid composition and its NH2-terminal amino acid sequence (thr-lys-asp-xxx-pro-ser-xxx-leu-thr-(thr)2-arg-ala-(leu)-(glu)-xxx-met- gly), and monospecific anti-glycoprotein IX antibody was prepared. The study outlines a useful approach for separating and characterizing glycoprotein IX, free from other members of the glycoprotein Ib complex.
Roth
G J
GJ
Medical Service, Veterans Administration Medical Center, Seattle, WA 98108.
Ozols
J
J
Nugent
D J
DJ
Williams
S A
SA
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000906:Antibodies / Q000276:immunology; D000941:Antigens / Q000276:immunology; D001792:Blood Platelets / Q000032:analysis*; D002845:Chromatography; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007163:Immunosorbent Techniques; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010980:Platelet Membrane Glycoproteins / Q000032:analysis / Q000097:blood* / Q000276:immunology
D000596:Amino Acids; D000906:Antibodies; D000941:Antigens; D046911:Macromolecular Substances; D010980:Platelet Membrane Glycoproteins