Distinct dystrophin mRNA species are expressed in embryonic and adult mouse skeletal muscle.
We have examined dystrophin mRNA in embryonic, newborn and adult mouse skeletal muscle. A discrete nerve-independent increase in mRNA size was observed between embryonic and adult stages, indicating that a developmentally regulated mRNA isoform switch occurs in the expression of the Duchenne muscular dystrophy (DMD) gene in skeletal muscle. These distinct mRNAs are most likely generated via selection of alternative transcriptional start sites or RNA processing pathways. In addition, denervation of adult muscle was without effect on the expression pattern.
Dickson
G
G
Department of Neurochemistry, Institute of Neurology, London, England.
Pizzey
J A
JA
Elsom
V E
VE
Love
D
D
Davies
K E
KE
Walsh
F S
FS
D000818:Animals; D000954:Antigens, Surface / Q000235:genetics; D015152:Blotting, Northern; D015815:Cell Adhesion Molecules; D015342:DNA Probes; D016189:Dystrophin; D005333:Fetus / Q000378:metabolism*; D005786:Gene Expression Regulation; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics; D006801:Humans; D051379:Mice; D009121:Muscle Denervation; D024510:Muscle Development; D009124:Muscle Proteins / Q000235:genetics*; D009132:Muscles / Q000196:embryology / Q000378:metabolism; D009137:Muscular Dystrophy, Animal / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic
D000954:Antigens, Surface; D015815:Cell Adhesion Molecules; D015342:DNA Probes; D016189:Dystrophin; D009124:Muscle Proteins; D012333:RNA, Messenger; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases