[Identification of the hemagglutinating antigens of the influenza virus by immunoenzyme analysis].
Highly active test sera detecting the presence of virus antigen both in concentrated and purified preparations and in allantoic virus cultures directly adsorbed on the solid phase have been proposed for successful identification and detection of influenza A and B virus variants. After direct sorption of purified and concentrated virus preparations, the test sera to influenza A (H1N1, H2N2, H3N2) virus detect the virus antigen in a concentration of 8 ng/ml, test sera to influenza B virus in a concentration of 40 ng/ml. After sorption on the solid phase of allantoic virus cultures the test sera detected influenza A virus antigen in a dose of 0.25-1 agglutinating units (AU), and antigen of influenza B virus in a dose of 1-2 AU.
Isaeva
E I
EI
Rovnova
Z I
ZI
Selivanov
Ia M
IaM
D000327:Adsorption; D000818:Animals; D000936:Antigen-Antibody Complex / Q000032:analysis; D006389:Hemagglutinins, Viral / Q000032:analysis*; D007106:Immune Sera / Q000276:immunology; D007124:Immunoenzyme Techniques; D009980:Influenza A virus / Q000276:immunology*; D009981:Influenza B virus / Q000276:immunology*; D011817:Rabbits; D051381:Rats
D000936:Antigen-Antibody Complex; D006389:Hemagglutinins, Viral; D007106:Immune Sera
Identifikatsiia gemaggliutiniruiushchikh antigenov virusa grippa metodom immunofermentnogo analiza.