title: Stimulation of RecA-mediated cleavage of phage phi 80 cI repressor by deoxydinucleotides.
abstract: The presence of either deoxyguanylyl-(3'----5')-deoxyguanosine (d(G-G] or deoxyadenylyl-(3'----5')-deoxyguanosine (d(A-G] greatly stimulates cleavage of the phage phi 80 cI repressor mediated by the Escherichia coli RecA protein in vitro. No other deoxydinucleoside monophosphate or riboguanylyl-(3'----5')-guanosine (r(G-G] affects the cleavage reaction. Neither the cleavage site of the phi 80 cI repressor nor the requirement for single-stranded DNA and ATP for cleavage is altered by d(G-G). Photoaffinity labeling experiments with 32P-labeled 5'-phosphoryl deoxyguanylyl deoxyguanosine (pd(G-G], which also stimulates cleavage, show that pd(G-G) bound to the repressor under the conditions in which the repressor is cleaved by RecA protein. The binding increases the affinity of the repressor for RecA protein and thus greatly stimulates repressor cleavage. The cleavage reactions of LexA and lambda cI repressors by RecA protein are not affected by d(G-G).
authors.affiliation: Department of Biology, Faculty of Science, Osaka University, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Eguchi
authors.forename: T
authors.initials: T
authors.lastname: Ogawa
authors.forename: H
authors.initials: H
authors.lastname: Ogawa
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism; D001435:Bacteriophages / Q000378:metabolism*; D003838:Deoxyadenine Nucleotides; D003849:Deoxyguanosine; D015226:Dinucleoside Phosphates / Q000378:metabolism; D004926:Escherichia coli; D007700:Kinetics; D009838:Oligodeoxyribonucleotides / Q000378:metabolism*; D011938:Rec A Recombinases / Q000378:metabolism*; D012097:Repressor Proteins / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism*; D014764:Viral Proteins / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D003838:Deoxyadenine Nucleotides; D015226:Dinucleoside Phosphates; D009838:Oligodeoxyribonucleotides; D012097:Repressor Proteins; D014157:Transcription Factors; D014764:Viral Proteins; C034298:deoxyguanylyl-(3'-5')-guanosine; C047743:deoxyadenylyl-(3'-5')-deoxyguanosine; D011938:Rec A Recombinases; D003849:Deoxyguanosine