Indirect immunofluorescent test on spore sampling preparations: a technique for diagnosis of individual mold allergies.
To calculate their respective exposure to mold spores, volumetric spore sampling was performed with Burkard personal air samplers in the homes of six asthmatic patients with different mold allergies, of three patients with baker's asthma and of four healthy controls. The spore samples were investigated with an indirect immunofluorescent test. Spore-specific IgE was detected in the serum of all the asthmatic patients with mold allergies, even in patients with only slightly increased or no detectable specific IgE in Phadezym-RAST. In serum of patients with baker's asthma and healthy controls, no spore-specific IgE was found.
Popp
W
W
Pulmonary Department, Vienna City Hospital, Austria.
Zwick
H
H
Rauscher
H
H
D000908:Antibodies, Fungal / Q000302:isolation & purification*; D001249:Asthma / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006801:Humans; D006967:Hypersensitivity / Q000175:diagnosis* / Q000276:immunology; D007073:Immunoglobulin E / Q000302:isolation & purification; D013172:Spores, Fungal / Q000276:immunology* / Q000302:isolation & purification
D000908:Antibodies, Fungal; D007073:Immunoglobulin E