Expression of the human apolipoprotein AI gene fused to the E. coli gene for beta-galactosidase.
The human apoAI gene was expressed in E. coli by in-frame fusion to a modified beta-galactosidase gene present in plasmid pUR291. The fused beta-galactosidase-apoAI gene product was expressed at a high level and was recognized by an anti-human apoAI antiserum. Besides the fused protein, at least one degradation product having an Mr similar to that of beta-galactosidase was present in high amounts in bacterial extracts. These results and those of a pulse-chase experiment indicate that degradation took place only in the apoAI moiety of the chimeric protein.
Lorenzetti
R
R
Sidoli
A
A
Palomba
R
R
Monaco
L
L
Martineau
D
D
Lappi
D A
DA
Soria
M
M
D016632:Apolipoprotein A-I; D001054:Apolipoproteins A / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004274:DNA, Recombinant / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics*; D005696:Galactosidases / Q000235:genetics*; D005798:Genes, Bacterial; D005809:Genes, Regulator; D006801:Humans; D008075:Lipoproteins, HDL / Q000235:genetics*; D008099:Liver / Q000378:metabolism; D010957:Plasmids; D001616:beta-Galactosidase / Q000235:genetics*
D016632:Apolipoprotein A-I; D001054:Apolipoproteins A; D004274:DNA, Recombinant; D008075:Lipoproteins, HDL; D005696:Galactosidases; D001616:beta-Galactosidase