title: The cdc30 mutation in Saccharomyces cerevisiae affects phosphoglucose isomerase, the cell cycle and sporulation.
abstract: Spontaneous revertants of the cdc30 mutation in Saccharomyces cerevisiae simultaneously regained the ability to grow and divide at 36.5 degrees C on glucose-containing media along with a more thermostable phosphoglucose isomerase (PGI). An independently isolated allele of cdc30 gave a similar phenotype to that previously described including temperature-sensitivity of PGI. Isoelectric focussing allowed the separation of two isoenzymes of PGI. These results all support the idea that two genes--PGI1 and CDC30--are responsible for PGI activity in yeast. Diploid strains homozygous for the cdc30 mutation sporulated poorly in potassium acetate irrespective of whether the cells had previously been cultured at a temperature that was permissive or restrictive for cell cycle progression. This was not surprising because a strain defective in PGI would not be expected to be able to complete the gluconeogenic events of sporulation.
authors.affiliation: Department of Microbiology, University College Cardiff, UK.
authors.forename: J R
authors.initials: JR
authors.lastname: Dickinson
authors.forename: M E
authors.initials: ME
authors.lastname: Smith
authors.forename: T R
authors.initials: TR
authors.lastname: Swanson
authors.forename: A S
authors.initials: AS
authors.lastname: Williams
authors.forename: J M
authors.initials: JM
authors.lastname: Wingfield
mesh_terms: D000483:Alleles; D002453:Cell Cycle; D005956:Glucose-6-Phosphate Isomerase / Q000235:genetics* / Q000302:isolation & purification; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000302:isolation & purification; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics / Q000502:physiology; D013172:Spores, Fungal
chemical_list: D007527:Isoenzymes; D005956:Glucose-6-Phosphate Isomerase