title: Immunoaffinity isolation of a sialyl-Le(a) oligosaccharide from human milk.
abstract: A cancer-associated antigen, sialyl-Le(a) oligosaccharide, was isolated from human milk using a monoclonal antibody recognizing carbohydrate moieties of mucin-type glycoproteins. The structure was identified as: (Formula: see text) based on 500-MHz 1H-NMR spectroscopy. This oligosaccharide comprises 0.07% of sialyloligosaccharides in human milk. The NMR spectra of two fellow oligosaccharides, Le(a) oligosaccharide (or lacto-N-fucopentaose II) and LS-tetrasaccharide a, are also given.
authors.affiliation: Department of Biological Chemistry, Faculty of Pharmaceutical Sciences, Kyoto University.
authors.forename: H
authors.initials: H
authors.lastname: Kitagawa
authors.forename: H
authors.initials: H
authors.lastname: Nakada
authors.forename: Y
authors.initials: Y
authors.lastname: Numata
authors.forename: A
authors.initials: A
authors.lastname: Kurosaka
authors.forename: S
authors.initials: S
authors.lastname: Fukui
authors.forename: I
authors.initials: I
authors.lastname: Funakoshi
authors.forename: T
authors.initials: T
authors.lastname: Kawasaki
authors.forename: K
authors.initials: K
authors.lastname: Shimada
authors.forename: F
authors.initials: F
authors.lastname: Inagaki
authors.forename: I
authors.initials: I
authors.lastname: Yamashina
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000302:isolation & purification*; D002846:Chromatography, Affinity / Q000379:methods; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007163:Immunosorbent Techniques; D009682:Magnetic Resonance Spectroscopy; D008895:Milk, Human / Q000378:metabolism*; D009077:Mucins / Q000096:biosynthesis; D009844:Oligosaccharides / Q000302:isolation & purification*
chemical_list: D000911:Antibodies, Monoclonal; D015295:Antigens, Tumor-Associated, Carbohydrate; D009077:Mucins; D009844:Oligosaccharides; C058981:sialyl-Le(a) oligosaccharide