Preservation of capsular material of streptococcal cells by specific lectins determined by immunoelectron microscopy.
We describe the use of lectins as specific stabilizing agents for the polysaccharide capsular components of two Gram-positive bacteria, Streptococcus agalactiae and Streptococcus bovis. Treatment of bacterial suspensions with wheatgerm agglutinin and concanavalin A allowed better morphological preservation as well as immunoelectron microscopic localization of a capsular component (lipoteichoic acid) by employing specific antibodies and the protein A-gold technique. Data obtained indicate that lectins are useful agents in preserving highly water-soluble capsular components during the electron microscopy procedures for both unembedded and embedded samples.
Molinari
A
A
Department of Ultrastructures, Istituto Superiore di Sanità, Rome, Italy.
Orefici
G
G
Donelli
G
G
Von Hunolstein
C
C
Paradisi
S
S
Arancia
G
G
D000818:Animals; D003208:Concanavalin A; D007150:Immunohistochemistry; D008070:Lipopolysaccharides / Q000032:analysis / Q000276:immunology; D008854:Microscopy, Electron / Q000379:methods*; D011309:Preservation, Biological; D013292:Streptococcus agalactiae / Q000032:analysis / Q000648:ultrastructure*; D013682:Teichoic Acids / Q000032:analysis / Q000276:immunology; D014909:Wheat Germ Agglutinins
D008070:Lipopolysaccharides; D013682:Teichoic Acids; D014909:Wheat Germ Agglutinins; D003208:Concanavalin A; C009900:lipoteichoic acid