Vitamin E exerts antiaggregatory effects without inhibiting the enzymes of the arachidonic acid cascade in platelets.
Vitamin E (alpha-tocopherol) and tocopherol acetate produced a slightly increased amount of thromboxane in treated compared to untreated platelets. In tocopherol acetate-treated platelets significantly more lipoxygenase products were produced. alpha-tocopherol induced an increased, but not significant, production of thromboxane B2 during blood clotting. alpha-tocopherol was not found to affect platelet phospholipase activity as determined by its effect on the release of labelled arachidonic acid from platelet phospholipids by challenging the platelets with calcium ionophore A23,187. alpha-tocopherol potentiated the incorporation of labelled arachidonate in the platelet phospholipids. Inspite of having no effect on the arachidonic acid cascade in platelets, alpha-tocopherol inhibited aggregation induced by several aggregating agents including A23,187. Inhibition of aggregation may be explained by the ability of alpha-tocopherol to inhibit intracellular mobilization of sequestered calcium from the dense tubular system to the cytoplasm.
Srivastava
K C
KC
D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000097:blood*; D001777:Blood Coagulation; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism*; D002118:Calcium / Q000097:blood; D006801:Humans; D066298:In Vitro Techniques; D010743:Phospholipids / Q000097:blood; D010974:Platelet Aggregation / Q000187:drug effects*; D013929:Thromboxane B2 / Q000096:biosynthesis; D014810:Vitamin E / Q000494:pharmacology*
D001095:Arachidonic Acids; D010743:Phospholipids; D014810:Vitamin E; D016718:Arachidonic Acid; D013929:Thromboxane B2; D002118:Calcium