[Isolation and characteristics of monoclonal antibodies to influenza virus types A and B].
Monoclonal antibodies (MCA) to influenza type A (10F) and B (5H and 6H) viruses have been prepared. By immunoblotting method, MCA 10F were found to be specific for NP-protein of influenza A virus, and MCA 5H and 6H to be specific for hemagglutinin of influenza B virus. It was established that the 10F clone interacted with all the investigated influenza A virus strains with different antigenic formulae (H1N1, H2N2, H3N2) and could be used for typing of this virus type. Clones 5H and 6H react specifically with hemagglutinins of influenza B viruses isolated in 1940, 1979, and 1983 which makes them useful for diagnosis of influenza B.
Busse
T L
TL
Khristova
M L
ML
Sokolova
M V
MV
Kharitonenkov
I G
IG
Saranpa
M
M
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D006389:Hemagglutinins, Viral / Q000032:analysis; D006825:Hybridomas / Q000276:immunology; D007114:Immunization; D007117:Immunization, Secondary; D015151:Immunoblotting; D007124:Immunoenzyme Techniques; D009980:Influenza A virus / Q000276:immunology*; D009981:Influenza B virus / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C
D000911:Antibodies, Monoclonal; D006389:Hemagglutinins, Viral
Poluchenie i kharakteristika monoklonal'nykh antitel k virusam grippa tipov A i B.