title: HTLV-III serology in hemophilia: relationship with immunologic abnormalities.
abstract: We investigated the relationship of the presence of antibodies to HTLV-III and immunologic abnormalities in patients with hemophilia. Serum antibodies to HTLV-III were analyzed by ELISA assay, immunoprecipitation of labeled cell extracts, and immunoprecipitation of purified HTLV-III p24. Thirty-four (61%) of the total group (n = 56) had antibody to HTLV-III; 34 (76%) of 45 patients given commercial factor VIII preparations were seropositive, compared with none of 11 patients treated exclusively with cryoprecipitate obtained from volunteer blood donors. Of patients who were seropositive for HTLV-III antibody, 94% had abnormal T4/T8 ratios, and 33% of those whose serum was antibody negative had abnormal T4/T8 ratios; five patients, each antibody positive, have lymphadenopathy syndrome. Sequential studies in a subset of patients indicate that there is a changing pattern of antibody production to HTLV-III antigens after seroconversion.
authors.forename: J C
authors.initials: JC
authors.lastname: Gill
authors.forename: J E
authors.initials: JE
authors.lastname: Menitove
authors.forename: P R
authors.initials: PR
authors.lastname: Anderson
authors.forename: J T
authors.initials: JT
authors.lastname: Casper
authors.forename: S G
authors.initials: SG
authors.lastname: Devare
authors.forename: C
authors.initials: C
authors.lastname: Wood
authors.forename: S
authors.initials: S
authors.lastname: Adair
authors.forename: J
authors.initials: J
authors.lastname: Casey
authors.forename: C
authors.initials: C
authors.lastname: Scheffel
authors.forename: R R
authors.initials: RR
authors.lastname: Montgomery
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000150:complications; D000914:Antibodies, Viral / Q000032:analysis*; D000917:Antibody Formation; D002478:Cells, Cultured; D003450:Cryoglobulins / Q000627:therapeutic use; D004340:Drug Contamination; D004797:Enzyme-Linked Immunosorbent Assay; D005169:Factor VIII / Q000627:therapeutic use; D015483:HIV Antibodies; D006467:Hemophilia A / Q000276:immunology* / Q000628:therapy; D006801:Humans; D007457:Iodine Radioisotopes; D007958:Leukocyte Count; D008297:Male; D010976:Platelet Count; D013601:T-Lymphocytes
chemical_list: D000914:Antibodies, Viral; D003450:Cryoglobulins; D015483:HIV Antibodies; D007457:Iodine Radioisotopes; D005169:Factor VIII