Small zone gel chromatography of interacting systems: theoretical and experimental evaluation of elution profiles for kinetically controlled macromolecule-ligand reactions.
A phenomenological theory of small zone gel chromatography is elaborated for kinetically controlled macromolecule-ligand interactions. Chromatography, direct binding experiments, and rate measurements are used for successful comparison of experimental behavior with theoretical elution profiles for the interaction of MAP-2 with two synthetic peptide fragments from the C-terminal moieties of alpha- and beta-tubulin subunits. The results of this study provide guidelines for interpretation of experimental small zone elution profiles of total ligand.
Cann
J R
JR
Department of Biochemistry/Biophysics/Genetics, University of Colorado Health Sciences Center, Denver 80262.
York
E J
EJ
Stewart
J M
JM
Vera
J C
JC
Maccioni
R B
RB
D000089:Acetic Anhydrides; D001704:Biopolymers; D002850:Chromatography, Gel / Q000379:methods*; D007700:Kinetics; D008024:Ligands; D046911:Macromolecular Substances; D008433:Mathematics; D008869:Microtubule-Associated Proteins / Q000302:isolation & purification*; D008962:Models, Theoretical; D011865:Radioisotope Dilution Technique; D014316:Tritium; D014404:Tubulin / Q000302:isolation & purification*
D000089:Acetic Anhydrides; D001704:Biopolymers; D008024:Ligands; D046911:Macromolecular Substances; D008869:Microtubule-Associated Proteins; D014404:Tubulin; D014316:Tritium; C031800:acetic anhydride