Tyrosine sulfation in precursors of collagen V.
Radioactive labeling of p-collagens V, collagens V, and, to a small extent, of procollagen V occurred when [35S]sulfate was incubated with tendons or primary tendon cell cultures, or blood vessels and crops of 17- to 19-day-old chick embryos, or with lung slices from neonatal rats. Most or all of this label is in the form of 1 or more sulfated tyrosine residues/chain of p alpha 1(V), alpha 1(V), p alpha 1'(V), alpha 1'(V), p alpha 2(V), and alpha 2(V), and it remains attached through purification by dialysis, ammonium sulfate precipitation, CsCl-GdnCl2 equilibrium buoyant density and velocity sedimentations, ion-exchange chromatography, and sodium dodecyl sulfate gel electrophoresis. Radioactive tyrosine sulfate was identified in alkaline hydrolysates of these collagen V chains, after labeling the tissues with either [35S]sulfate or [3H]tyrosine, by electrophoretic and chromatographic comigration with a tyrosine sulfate standard. Tunicamycin A1, which inhibits the attachment of N-linked complex carbohydrate, did not interfere with the sulfation process. The tyrosine sulfate is located in a noncollagenous domain, which is probably adjacent to the amino end of the collagen helix, and is retained throughout the physiological proteolytic processing of procollagens V. After digestion with Staphylococcus aureus V8 protease, 35S-labeled p alpha 1(V) and alpha 1(V) chains gave the same map of labeled peptides, and this differed from the map given by p alpha 1'(V) and alpha 1'(V) chains. Little sulfation of p alpha 2(V) and alpha 2(V) chains occurs. The implications of these observations for the structure and properties of procollagens V and their derivatives are considered.
Fessler
L I
LI
Brosh
S
S
Chapin
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S
Fessler
J H
JH
D000818:Animals; D000971:Antineoplastic Combined Chemotherapy Protocols; D001808:Blood Vessels / Q000378:metabolism; D002499:Centrifugation, Density Gradient; D011232:Chemical Precipitation; D002642:Chick Embryo; D002848:Chromatography, DEAE-Cellulose; D003094:Collagen / Q000378:metabolism*; D003561:Cytarabine / Q000378:metabolism; D003630:Daunorubicin / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D010450:Endopeptidases / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D010434:Pepsin A / Q000378:metabolism; D010446:Peptide Fragments / Q000378:metabolism; D011239:Prednisolone / Q000378:metabolism; D011347:Procollagen / Q000378:metabolism*; D051381:Rats; D012697:Serine Endopeptidases; D013431:Sulfates / Q000378:metabolism*; D013462:Sulfur Radioisotopes; D013710:Tendons / Q000378:metabolism; D014443:Tyrosine / Q000378:metabolism*; D014750:Vincristine / Q000378:metabolism
D010446:Peptide Fragments; D011347:Procollagen; D013431:Sulfates; D013462:Sulfur Radioisotopes; D003561:Cytarabine; D014443:Tyrosine; D014750:Vincristine; D003094:Collagen; D011239:Prednisolone; D010450:Endopeptidases; D012697:Serine Endopeptidases; C028344:glutamyl endopeptidase; D010434:Pepsin A; D003630:Daunorubicin