title: Specificity in calcium oxalate adherence to papillary epithelial cells in cultures.
abstract: Attachment of microcrystallites to cellular membranes may be an important component of the pathophysiology of many diseases including urolithiasis. This study attempts to characterize the interaction of calcium oxalate (CaOx) crystals and apatite (AP) crystals with renal papillary collecting tubule (RPCT) cells in primary culture. Primary cultures of RPCT cells showed the characteristic monolayer growth with sporadically interspersed clumped cells. Cultures were incubated with [14C]CaOx crystals, and the crystals that bound were quantified by microscopy and adherent radioactivity. Per unit of cross-sectional area, 32 times more CaOx crystals were bound to the clumps than to the monolayer. CaOx adherence demonstrated concentration-dependent saturation with a beta value (fraction of cell culture area binding CaOx crystals) of 0.179 and a 1/alpha ox value (maximum micrograms of crystallites adhering to 1 cm2 of binding area) of 287 micrograms/cm2. On coincubation with AP crystals, CaOx binding demonstrated concentration-dependent inhibition with a 1/alpha AP value of 93 micrograms/cm2. Microcrystallite adherence to RPCT cells demonstrates selectivity for cellular clumps, saturation, and inhibition. These features suggest specific binding.
authors.affiliation: Veterans Administration Medical Center, Medical College of Wisconsin, Milwaukee 53295.
authors.forename: R J
authors.initials: RJ
authors.lastname: Riese
authors.forename: J W
authors.initials: JW
authors.lastname: Riese
authors.forename: J G
authors.initials: JG
authors.lastname: Kleinman
authors.forename: J H
authors.initials: JH
authors.lastname: Wiessner
authors.forename: G S
authors.initials: GS
authors.lastname: Mandel
authors.forename: N S
authors.initials: NS
authors.lastname: Mandel
mesh_terms: D000818:Animals; D001031:Apatites / Q000378:metabolism; D002129:Calcium Oxalate / Q000378:metabolism*; D002478:Cells, Cultured; D003460:Crystallization; D004848:Epithelium / Q000378:metabolism; D007679:Kidney Medulla / Q000378:metabolism*; D008297:Male; D008433:Mathematics; D008858:Microscopy, Phase-Contrast; D008859:Microscopy, Polarization; D051381:Rats; D011919:Rats, Inbred Strains; D014961:X-Ray Diffraction
chemical_list: D001031:Apatites; D002129:Calcium Oxalate