title: Resolution of the two components of macrophage inflammatory protein 1, and cloning and characterization of one of those components, macrophage inflammatory protein 1 beta.
abstract: A number of macrophage-derived mediators have been implicated in the vascular changes of inflammation. We recently reported the isolation of a novel monokine, macrophage inflammatory protein 1 (MIP-1), which causes local inflammatory responses in vivo, and induces superoxide production by neutrophils in vitro. Purified native MIP-1 comprises two peptides with very similar physical characteristics. We report here the resolution of MIP-1 into component peptides by SDS-hydroxylapatite chromatography, and compare the NH2-terminal sequences of the two peptides, now referred to as MIP-1 alpha and MIP-1 beta. A synthetic oligonucleotide probe pool corresponding to the NH2-terminal amino acid sequence of MIP-1 beta was used to isolate a cDNA clone containing its coding sequence. The sequence codes for a 109 amino acid-long polypeptide, of which 69 amino acids correspond to the mature product. Comparison of this MIP-1 beta cDNA with our previously cloned MIP-1 alpha sequence reveals that the MIP-1 peptides, members of a growing family of potential inflammatory mediators, are distinct but highly homologous (58.9% sequence identity) products of different genes.
authors.affiliation: Laboratory of Medical Biochemistry, Rockefeller University, New York, New York 10021.
authors.forename: B
authors.initials: B
authors.lastname: Sherry
authors.forename: P
authors.initials: P
authors.lastname: Tekamp-Olson
authors.forename: C
authors.initials: C
authors.lastname: Gallegos
authors.forename: D
authors.initials: D
authors.lastname: Bauer
authors.forename: G
authors.initials: G
authors.lastname: Davatelis
authors.forename: S D
authors.initials: SD
authors.lastname: Wolpe
authors.forename: F
authors.initials: F
authors.lastname: Masiarz
authors.forename: D
authors.initials: D
authors.lastname: Coit
authors.forename: A
authors.initials: A
authors.lastname: Cerami
mesh_terms: D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D002630:Chemotactic Factors / Q000235:genetics*; D002845:Chromatography; D016209:Interleukin-8; D051379:Mice; D008969:Molecular Sequence Data; D012689:Sequence Homology, Nucleic Acid
chemical_list: D002630:Chemotactic Factors; D016209:Interleukin-8