title: Structure-activity relationship in vinculin: an IR/attenuated total reflection spectroscopic and film balance study.
abstract: Surfacing and membrane-penetrating ability of vinculin and bovine serum albumin have been studied on a macroscopic level by means of a Langmuir film balance and on a molecular level by means of infrared attenuated total reflection spectroscopy. It is suggested that the driving force of the nonspontaneous process of membrane penetration by native vinculin is the spontaneous formation of rigid vinculin monolayers in the membrane. Lateral adhesion of vinculin molecules results from the formation of intermolecular pleated-sheet structures. Vinculin surface activity was found to result from an alpha-helical segment oriented approximately perpendicular to plane of the membrane. There is a conformational equilibrium between this helix and random structure. High ionic strength (110 mM) favors helix formation that leads to the greater than 100-fold enhancement of surfacing velocity relative to the velocity observed at a lower ionic strength (10 mM). Vinculin has a second helical segment oriented parallel to the plane of the membrane that is in a conformational equilibrium with the pleated-sheet structure.
authors.forename: U P
authors.initials: UP
authors.lastname: Fringeli
authors.forename: P
authors.initials: P
authors.lastname: Leutert
authors.forename: H
authors.initials: H
authors.lastname: Thurnhofer
authors.forename: M
authors.initials: M
authors.lastname: Fringeli
authors.forename: M M
authors.initials: MM
authors.lastname: Burger
mesh_terms: D000577:Amides; D000818:Animals; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002645:Chickens; D005895:Gizzard, Avian; D006863:Hydrogen-Ion Concentration; D008563:Membrane Lipids; D009124:Muscle Proteins; D009994:Osmolar Concentration; D010713:Phosphatidylcholines; D013055:Spectrophotometry, Infrared; D013329:Structure-Activity Relationship; D013499:Surface Properties; D016596:Vinculin
chemical_list: D000577:Amides; D008563:Membrane Lipids; D009124:Muscle Proteins; D010713:Phosphatidylcholines; D016596:Vinculin