Circulating antibodies and antigens in Presbytis monkeys infected with the filarial parasite Wuchereria bancrofti.
Levels of circulating filarial antigen, and humoral antibody to other defined antigenic targets, were measured over the course of experimental infection of three Presbytis cristatus monkeys with Wuchereria bancrofti. Circulating antigen levels, measured with an anti-phosphorylcholine monoclonal antibody, varied widely although all animals were positive for some period of the infection. Circulating antigen levels tended to be inversely related to the titre of anti-phosphorylcholine antibody, and this trend was maintained even following acid dissociation and inactivation of immune complexed host antibody. Other antibody specificities were measured by Western blotting against somatic proteins and immunoprecipitation of surface antigens. Amongst somatic antigens, targets between 14,000 and 200,000 daltons were recognised by monkey antibodies, but no correlation with infection status could be discerned. Likewise when measuring the response to the 29,000 dalton major adult surface glycoprotein, one animal produced a rapid response but the others did not recognise it until late in infection. In general, the experimental findings are characterised by wide variability between individuals, as may perhaps be expected in a primate host for a human spectral disease.
Maizels
R M
RM
Department of Pure and Applied Biology, Imperial College of Science and Technology, London, UK.
Morgan
T M
TM
Gregory
W F
WF
Selkirk
M E
ME
Purnomo
Sukartono
Partono
F
F
D000818:Animals; D000909:Antibodies, Helminth / Q000032:analysis*; D000911:Antibodies, Monoclonal / Q000276:immunology; D000947:Antigens, Helminth / Q000032:analysis* / Q000276:immunology; D000954:Antigens, Surface / Q000032:analysis / Q000276:immunology; D015153:Blotting, Western; D002097:C-Reactive Protein / Q000032:analysis; D002520:Cercopithecidae / Q000469:parasitology; D004605:Elephantiasis, Filarial / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005368:Filariasis / Q000276:immunology*; D008297:Male; D010767:Phosphorylcholine / Q000276:immunology; D014957:Wuchereria / Q000276:immunology*; D014958:Wuchereria bancrofti / Q000276:immunology*
D000909:Antibodies, Helminth; D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth; D000954:Antigens, Surface; D010767:Phosphorylcholine; D002097:C-Reactive Protein