Epidermal growth factor induces internalization but not degradation of the epidermal growth factor receptor in a human breast cancer cell line.
Metabolism of the epidermal growth factor (EGF) receptor was studied in the MDA-MB-231 human breast cancer cell line. As in normal fibroblasts the EGF receptor from MDA-MB-231 cells was synthesized from a Mr = 160,000 precursor and tunicamycin treatment of cells resulted in accumulation of a Mr = 130,000 polypeptide. Unlike normal fibroblasts in which a Mr = 170,000 mature form of the EGF receptor was found, MDA-MB-231 cells contained a Mr = 172,000 mature form. Addition of EGF to MDA-MB-231 cells led to rapid internalization of EGF receptors, however, internalization did not affect receptor half-life and receptors did not recycle to the cell surface. EGF receptors could be visualized by immunofluorescence and remained sequestered in intracellular membranous structures following internalization. EGF was degraded slowly by MDA-MB-231 cells relative to degradation of EGF by normal cells. A high endogenous level of in vivo phosphorylation of threonine 654 of the EGF receptor was found in MDA-MB-231 cells and treatment of cells with 12-O-tetradecanoyl-phorbol-13-acetate (TPA) further stimulated phosphorylation of this residue. EGF induced receptor internalization resulted in dephosphorylation of threonine 654. The significance of these unusual properties of EGF receptor metabolism in MDA-MB-231 cells is discussed.
Decker
S J
SJ
Rockefeller University, New York, NY 10021.
D001943:Breast Neoplasms / Q000378:metabolism*; D004247:DNA / Q000096:biosynthesis; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D066246:ErbB Receptors / Q000032:analysis / Q000378:metabolism*; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D010766:Phosphorylation; D014407:Tumor Cells, Cultured
D004815:Epidermal Growth Factor; D004247:DNA; D066246:ErbB Receptors