Agent-specific ras oncogene activation in rat thyroid tumours.
Activated ras oncogenes have been detected in a number of rodent tumour model systems (Balmain et al., 1986; Barbacid 1987), in which chemical carcinogens have been the most widely used inducing agents. We have investigated the involvement of activated oncogenes in a single well-characterised tumour model, rat thyroid neoplasia, produced not only by chemical carcinogen (nitrosomethylurea, NMU) and by ionizing radiation but also by elevated trophic stimulation alone. We have indentified a striking specificity in patterns of ras oncogene activation, with exclusively Ha-ras activated in 87% of NMU-induced tumours and exclusively Ki-ras activated in 60% of radiation-induced tumours.
Lemoine
N R
NR
Department of Pathology, University of Wales College of Medicine, Heath Park, Cardiff, UK.
Mayall
E S
ES
Williams
E D
ED
Thurston
V
V
Wynford-Thomas
D
D
D000640:Amitrole / Q000494:pharmacology; D000818:Animals; D015139:Blotting, Southern; D004273:DNA, Neoplasm / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D011905:Genes, ras / Q000187:drug effects; D008770:Methylnitrosourea / Q000494:pharmacology; D051381:Rats; D013964:Thyroid Neoplasms / Q000139:chemically induced / Q000235:genetics* / Q000473:pathology; D014162:Transfection
D004273:DNA, Neoplasm; D008770:Methylnitrosourea; D000640:Amitrole