Determination of ochratoxin A by monoclonal antibody-based enzyme immunoassay.
A simple and rapid indirect enzyme-linked immunosorbent assay was developed for the quantitative determination of ochratoxin A in barley after the successful production of a high affinity, specific monoclonal antibody. A rapid sample cleanup was achieved by extracting ochratoxin A from barley with chloroform and partitioning the toxin into bicarbonate buffer; the buffer solution was then added directly to the assay plate and ochratoxin A content was assessed. Recoveries were greater than 85% and detection limits were 5 micrograms ochratoxin A/kg barley.
Candlish
A A
AA
University of Strathclyde, Department of Bioscience and Biotechnology, Glasgow, Scotland.
Stimson
W H
WH
Smith
J E
JE
D000911:Antibodies, Monoclonal; D002478:Cells, Cultured; D046508:Culture Techniques; D004797:Enzyme-Linked Immunosorbent Assay; D001467:Hordeum / Q000032:analysis; D007124:Immunoenzyme Techniques; D009793:Ochratoxins / Q000032:analysis*; D011506:Proteins / Q000032:analysis
D000911:Antibodies, Monoclonal; D009793:Ochratoxins; D011506:Proteins; C025589:ochratoxin A