A rapid microassay for detecting hepatitis B surface antigen by dot enzyme immunoassay.
This study describes a dot enzyme immunoassay (Dot-EIA) for detecting hepatitis B surface antigen (HBsAg). The results demonstrated that the detection level of this assay for HBsAg was 1.5 ng/ml; no false-positive or -negative readings were observed. Also, this Dot-EIA had some advantages over standard EIA: (1) antiserum could be directly and immediately bound on nitrocellulose paper set into microfiltration apparatus, (2) the paper could be easily washed under reduced pressure using a water aspirator, (3) all assay steps could be performed at room temperature within 2 h, (4) the well-defined brown spots could be evaluated by both visual observation and densitometric reading. The Dot-EIA reported here may be useful for rapid diagnosis and screening of HBsAg in serum.
Matsumura
K
K
Yamaguchi Prefectural Research Institute of Health, Japan.
Wakatsuki
S
S
Iwasaki
A
A
Endo
R
R
Tanaka
K
K
D001360:Avidin; D001710:Biotin; D003101:Collodion; D006509:Hepatitis B / Q000175:diagnosis / Q000276:immunology; D006514:Hepatitis B Surface Antigens / Q000097:blood*; D006801:Humans; D007124:Immunoenzyme Techniques; D011786:Quality Control
D006514:Hepatitis B Surface Antigens; D001360:Avidin; D001710:Biotin; D003101:Collodion