Improved method for high-yield excystation and purification of infective sporozoites of Eimeria spp.
This report describes a new, gentle procedure for rapid and efficient excystation of large numbers of infective sporozoites of Eimeria vermiformis and Eimeria stiedai. Excysted sporozoites are purified using modifications of a previously described ion-exchange chromatography method. The procedure avoids physical breakage of oocysts and results in greater than 70% recovery of the sporozoites present as sporulated oocysts (i.e. 5-6 sporozoites per sporulated oocyst). The recovered sporozoites are greater than 95% pure and are infective in vivo. We routinely isolate greater than 2 x 10(8) sporozoites without the use of specialized or expensive equipment.
Hosek
J E
JE
Department of Pathobiology, College of Veterinary Medicine, University of Illinois, Urbana 61801.
Todd
K S
KS
Kuhlenschmidt
M S
MS
D000818:Animals; D002852:Chromatography, Ion Exchange / Q000379:methods; D004539:Eimeria / Q000254:growth & development / Q000302:isolation & purification* / Q000472:pathogenicity; D007700:Kinetics; D051379:Mice; D008828:Microbiological Techniques; D013696:Temperature