[Combined effect of interferons alpha, beta and gamma on tumor growth in vitro].
The antiproliferative effect of human recombinant interferon alpha, beta and gamma was studied in vitro in combination. Growth of human gastric cancer cell lines and a pancreatic cancer cell line was inhibited markedly by combination of interferons beta and gamma or a combination of all three interferon types. Combination of low-dose interferons (50-100 units/ml) was more effective than a high dose of single interferon. Moreover, human pancreatic cancer cells were completely eliminated after incubation with 5-fluorouracil (0.001 microgram/ml) or methotrexate (0.1 microgram/ml) combined with interferons beta and gamma (50 units/ml each). These results suggested that combination of interferons beta and gamma could be the most effective interferon therapy for cancer.
Kimoto
Y
Y
D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D004359:Drug Therapy, Combination; D005472:Fluorouracil / Q000008:administration & dosage; D006801:Humans; D007370:Interferon Type I / Q000008:administration & dosage*; D007371:Interferon-gamma / Q000008:administration & dosage*; D008727:Methotrexate / Q000008:administration & dosage; D051379:Mice; D010190:Pancreatic Neoplasms / Q000473:pathology / Q000628:therapy*; D013274:Stomach Neoplasms / Q000473:pathology / Q000628:therapy*
D007370:Interferon Type I; D007371:Interferon-gamma; D005472:Fluorouracil; D008727:Methotrexate