H-Y responses of non-obese diabetic (NOD) mice.
Female non-obese diabetic (NOD) mice were tested for their ability to make responses to the male-specific (H-Y) transplantation antigen. In vivo assessment of this ability was made using skin graft rejection. A proportion (60%) spontaneously rejected NOD male tail skin by 80 days post-transplantation. The detection of the generation of H-Y-specific cytotoxic T cells, following in vivo priming and secondary in vitro restimulation, was carried out using a conventional 51Cr release assay. Female NOD mice primed either by skin grafting, intraperitoneal (i.p.) or footpad (f.p.) injection of male NOD spleen cells could be induced to make anti-H-Y cytotoxic responses, but not every immunized mouse responded. The nature of the H-Y-reactive T cells was investigated further by the in vitro isolation of T-cell clones of which some were H-Y specific.
Chandler
P
P
Transplantation Biology Section, MRC Clinical Research Centre, Harrow, Middlesex, U.K.
Fairchild
S
S
Simpson
E
E
D000818:Animals; D002999:Clone Cells / Q000276:immunology; D003921:Diabetes Mellitus, Experimental / Q000235:genetics / Q000276:immunology*; D005260:Female; D006084:Graft Rejection; D006182:H-Y Antigen; D008297:Male; D051379:Mice; D008817:Mice, Mutant Strains; D016038:Skin Transplantation; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology
D006182:H-Y Antigen