Express analysis of protein amino acid sequences. Primary structure of Penicillium chrysogenum 152A guanyl-specific ribonuclease.
The complete amino acid sequence of Penicillium chrysogenum 152A guanyl-specific RNase has been established using automated Edman degradation of two non-fractionated peptide mixtures produced by tryptic and staphylococcal protease digests of the protein. The RNase contains 102 amino acid residues: His2, Arg3, Asp7, Asn8, Thr5, Ser11, Glu4, Gln2, Pro4, Gly11, Ala13, Cys4, Val8, Ile3, Leu3, Tyr9, Phe5 (Mr 10 747).
Shlyapnikov
S V
SV
Bezborodova
S I
SI
Kulikov
V A
VA
Yakovlev
G I
GI
D000595:Amino Acid Sequence; D010450:Endopeptidases; D004722:Endoribonucleases / Q000032:analysis*; D008666:Metalloendopeptidases; D008722:Methods; D010407:Penicillium / Q000201:enzymology*; D010408:Penicillium chrysogenum / Q000201:enzymology*; D010455:Peptides / Q000032:analysis; D006163:Ribonuclease T1 / Q000032:analysis*; D014357:Trypsin
D010455:Peptides; D004722:Endoribonucleases; D006163:Ribonuclease T1; D010450:Endopeptidases; D014357:Trypsin; D008666:Metalloendopeptidases; C022653:auR protein, Staphylococcus aureus