Non-radioactive gene probes for the detection of tetracycline and/or minocycline resistance in staphylococci.
This report describes the development of a non-radioactive gene probe for the characterization of tetracycline and/or minocycline resistant staphylococci. Both radio-labelled and non-radioactive gene probes yielded a better characterization of the strains than the determination of minimal inhibitory concentration (MIC). A 100% correlation was found between radioactive and non-radioactive hybridization tests. The latter technique detected genes present in a single copy per bacterial genome, could be performed in 8 h, avoided radioactive hazards and autoradiography delay, and appeared therefore to be a useful tool for clinical and epidemiological studies.
Sakamoto
H
H
Laboratoire des Sondes Froides, Institute Pasteur, Paris, France.
Zilhao
R
R
Bismuth
R
R
Courvalin
P
P
Guesdon
J L
JL
D000818:Animals; D000911:Antibodies, Monoclonal; D015342:DNA Probes; D004269:DNA, Bacterial / Q000032:analysis; D007124:Immunoenzyme Techniques; D008911:Minocycline / Q000494:pharmacology*; D009693:Nucleic Acid Hybridization; D013211:Staphylococcus aureus / Q000235:genetics*; D013753:Tetracycline Resistance / Q000235:genetics*; D013754:Tetracyclines / Q000494:pharmacology*
D000911:Antibodies, Monoclonal; D015342:DNA Probes; D004269:DNA, Bacterial; D013754:Tetracyclines; D008911:Minocycline