title: Ras (proto)oncogene induces N-linked carbohydrate modification: temporal relationship with induction of invasive potential.
abstract: The effect of expression of the ras oncogene on protein glycosylation was studied. VSV G-protein and class I histocompatibility antigens were analysed to monitor ras-mediated changes in glycosylation. Transient expression of the c-Ha-ras oncogene, introduced into NIH 3T3 cells by the DEAE-dextran method, altered protein glycosylation within 25 h of transfection. The same result was obtained after dexamethasone-induced expression of p21-ras in stable NIH 3T3 transfectants containing either an activated Ha-ras oncogene or a normal N-ras proto-oncogene under control of the glucocorticoid-inducible MMTV promoter. The alteration of cell surface carbohydrates, induced by the ras (proto)oncogene and the subsequent acquisition of invasive potential, occurred prior to morphological transformation.
authors.affiliation: Division of Cellular Biochemistry, The Netherlands Cancer Institute, Amsterdam.
authors.forename: J G
authors.initials: JG
authors.lastname: Bolscher
authors.forename: M M
authors.initials: MM
authors.lastname: van der Bijl
authors.forename: J J
authors.initials: JJ
authors.lastname: Neefjes
authors.forename: A
authors.initials: A
authors.lastname: Hall
authors.forename: L A
authors.initials: LA
authors.lastname: Smets
authors.forename: H L
authors.initials: HL
authors.lastname: Ploegh
mesh_terms: D000818:Animals; D050260:Carbohydrate Metabolism; D002460:Cell Line; D002461:Cell Line, Transformed; D002471:Cell Transformation, Neoplastic; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005786:Gene Expression Regulation; D011905:Genes, ras; D006020:Glycopeptides / Q000378:metabolism; D006031:Glycosylation; D015395:Histocompatibility Antigens Class I / Q000378:metabolism; D007525:Isoelectric Focusing; D007700:Kinetics; D008562:Membrane Glycoproteins; D011506:Proteins / Q000378:metabolism*; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis; D016283:Proto-Oncogene Proteins p21(ras); D014162:Transfection; D014759:Viral Envelope Proteins / Q000378:metabolism
chemical_list: C028391:G protein, vesicular stomatitis virus; D006020:Glycopeptides; D015395:Histocompatibility Antigens Class I; D008562:Membrane Glycoproteins; D011506:Proteins; D011518:Proto-Oncogene Proteins; D014759:Viral Envelope Proteins; D016283:Proto-Oncogene Proteins p21(ras)