Expression of interleukin 2 receptors on interleukin 3-dependent cell lines.
Several mouse IL 3-dependent cell lines, IC2, LT4, FDC-P2, and PB-3C, derived from spleen or bone marrow cells were shown to express low affinity receptors for IL 2 (Kd; 0.5 to 8 X 10(-8) M). High affinity receptors for IL 2 were not detected on the IL 3-dependent cells within the experimental limitation of this study. The clones did not respond to IL 2 at all at the concentration as high as 25 micrograms/ml. The number of the receptors expressed on those clones was estimated to be 0.2 to 2 X 10(5)/cell, which is comparable with the number of those on IL 2-dependent T cell clones. Expression of IL 2 receptor was confirmed in mRNA levels for both IC2 and LT4 cells. A relatively low level expression of one (4.5 Kb) of four IL 2 receptor mRNA species was observed with those IL 3-dependent clones compared with IL 2-dependent T cells. It seems that these low affinity receptors may be expressed on IL 3-dependent cells that undergo differentiation or maturation in mast cell and some myeloid cell lineages.
Koyasu
S
S
Yodoi
J
J
Nikaido
T
T
Tagaya
Y
Y
Taniguchi
Y
Y
Honjo
T
T
Yahara
I
I
D000818:Animals; D001854:Bone Marrow Cells; D002460:Cell Line; D005260:Female; D007376:Interleukin-2 / Q000378:metabolism*; D007377:Interleukin-3; D008222:Lymphokines / Q000502:physiology*; D008407:Mast Cells / Q000378:metabolism; D051379:Mice; D008811:Mice, Inbred DBA; D051381:Rats; D011917:Rats, Inbred Lew; D011971:Receptors, Immunologic / Q000032:analysis* / Q000096:biosynthesis; D015375:Receptors, Interleukin-2; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000378:metabolism
D007376:Interleukin-2; D007377:Interleukin-3; D008222:Lymphokines; D011971:Receptors, Immunologic; D015375:Receptors, Interleukin-2