Antisera of designed specificity for subunits of guanine nucleotide-binding regulatory proteins.
Antisera were raised against purified subunits of regulatory GTP-binding proteins (G proteins) and against synthetic peptides that correspond to defined regions of G proteins. Peptide antisera were generated that recognized all alpha or all beta subunits from Gs, Gi, Go, and transducin; others recognized only Gs alpha or Go alpha. Such cross-reaction or complete specificity for a given alpha subunit was not obtained when purified subunits were injected. Peptide antisera were used to identify G protein subunits in selected tissue membrane preparations by immunoblots.
Mumby
S M
SM
Kahn
R A
RA
Manning
D R
DR
Gilman
A G
AG
D000818:Animals; D000918:Antibody Specificity; D002417:Cattle; D019204:GTP-Binding Proteins / Q000276:immunology*; D046911:Macromolecular Substances; D008565:Membrane Proteins / Q000276:immunology*; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000276:immunology; D011817:Rabbits; D015293:Transducin
D046911:Macromolecular Substances; D008565:Membrane Proteins; D010446:Peptide Fragments; D019204:GTP-Binding Proteins; D015293:Transducin