title: Binding of E. coli to human blood leukocytes.
abstract: The spontaneous binding of both piliated and non-piliated E. coli to human lymphocytes, monocytes, and polymorphonuclear leukocytes (PMN) was investigated. It was found that the piliated E. coli showed a higher binding percentage to lymphocytes than the nonpiliated bacteria. A specific antiserum raised in rabbit against E. coli strongly inhibited the binding of the homologous E. coli strain to lymphocytes and monocytes. On the other hand there was no difference in the binding of piliated and non-piliated E. coli to PMNs, and the specific E. coli antiserum did not inhibit the binding. The binding of E. coli to human lymphocytes obtained from healthy donors was investigated in a 3-week follow-up experiment. It was shown that there were significant differences between the binding of cells from different individuals, whereas there were no differences in the binding ability of the cells from the same individual in the 3-week follow up.
authors.affiliation: Peking Union Medical College, Beijing, People's Republic of China.
authors.forename: Y Z
authors.initials: YZ
authors.lastname: Guan
authors.forename: A
authors.initials: A
authors.lastname: Kharazmi
mesh_terms: D001422:Bacterial Adhesion; D004926:Escherichia coli / Q000472:pathogenicity*; D006801:Humans; D066298:In Vitro Techniques; D007962:Leukocytes / Q000382:microbiology*; D008214:Lymphocytes / Q000382:microbiology; D009000:Monocytes / Q000382:microbiology; D009504:Neutrophils / Q000382:microbiology