Novel responses of peripheral lymphocytes of cancer patients to chemical induction of sister chromatid exchanges.
Sensitivities to sister chromatid exchange (SCE) induction by chemicals of peripheral lymphocytes from 26 cancer patients were estimated under conditions identical to those for healthy humans which had been reported (Cancer Res., 43: 439-442, 1983). The sensitive individual was defined as one whose cells give a mean induced SCE frequency more than 2 standard deviation units above the population mean of induced SCEs in cells from the healthy humans. When cells were treated with 3-amino-1-methyl-5H-pyrido[4, 3-b]indole in the presence of rat liver S9 mix, 8 in 10 stomach cancer patients, 4 in 4 colon cancer patients, 3 in 9 lung cancer patients, 0 in 3 patients bearing other cancers, and 0 in 9 non-cancerous individuals were sensitive. The corresponding frequency of individuals in the healthy population, reported previously, was 1 in 33 persons. Thus, the frequency of sensitive individuals in the combined group of stomach and colon cancer patients was very significantly higher than were frequencies in control groups. Three in 10 patients with stomach cancer and 4 in 16 patients with other cancers were sensitive to induction of SCE by methyl methanesulfonate. Six in these 7 methyl methanesulfonate-sensitive patients were also 3-amino-1-methyl-5H-pyrido[4,3-b]indole sensitive. The frequency of methyl methanesulfonate-sensitive individuals in the healthy populations was 2 in 50. There was no patient who was sensitive to SCE induction by 4-nitroquinoline 1-oxide. The frequency was not significantly different from the healthy population, in which 3 in 50 persons were sensitive. These results suggest that a particular cancer correlates with the sensitivity of peripheral lymphocytes to SCE induction by particular chemicals.
Oikawa
A
A
Horaguchi
K
K
Sugawara
R
R
Kikuchi
J
J
Tohda
H
H
Takahashi
K
K
Wakui
A
A
Yamauchi
A
A
Nakada
T
T
D015112:4-Nitroquinoline-1-oxide / Q000494:pharmacology; D000368:Aged; D002243:Carbolines / Q000494:pharmacology; D003110:Colonic Neoplasms / Q000235:genetics / Q000473:pathology; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000473:pathology; D008214:Lymphocytes / Q000187:drug effects*; D008297:Male; D008741:Methyl Methanesulfonate / Q000494:pharmacology; D008875:Middle Aged; D009369:Neoplasms / Q000235:genetics* / Q000473:pathology; D012854:Sister Chromatid Exchange / Q000187:drug effects*; D013274:Stomach Neoplasms / Q000235:genetics / Q000473:pathology
D002243:Carbolines; D015112:4-Nitroquinoline-1-oxide; C019133:3-amino-1-methyl-5H-pyrido(4,3-b)indole; D008741:Methyl Methanesulfonate