[In vitro antibacterial activity of apalcillin on aerobic bacteria and the regression curve].
Minimal inhibitory concentrations (MICs) of apalcillin (APL) were evaluated by agar dilution on 1,201 bacterial strains isolated simultaneously in four university hospitals; agar diffusion tests (disks APL: 75 micrograms) were performed on these strains to establish relationship between MIC and zone diameters. For Enterobacteriaceae naturally non beta-lactamase-producing (E. coli and P. mirabilis), mode MIC was 0.5 microgram/ml; some acquired penicillinase-producing strains were only inhibited by concentrations greater than or equal to 16. Chromosomal penicillinase producing Klebsiella were inhibited by 2 to 8 micrograms/ml but APL was inactive on acquired penicillinase-producing strains. For chromosomal cephalosporinase-producing species (Enterobacter, Citrobacter, Serratia, indole + Proteus and Providencia) two populations of strains were observed: one sensitive and the second resistant to carboxypenicillins: on the first population, mode MIC of APL was 1 to 4 micrograms/ml; on the second MIC were generally greater than or equal to 64 micrograms/ml. P. aeruginosa strains sensitive to carboxypenicillins were inhibited by 1 and 2 micrograms/ml; this activity was diminished on strains resistant to these antibiotics (MIC APL 8-32). MIC of Acinetobacter varied to 0.25 to greater than 128 with a majority of strains inhibited by 4 to 64 micrograms/ml. APL was active against non penicillinase producing Staphylococci; mode MIC was 2 micrograms/ml for Enterococci. Correlation coefficient of regression curve was 0.87. For critical concentrations less than or equal to 8 and greater than 64 micrograms/ml, critical diameters could be greater than or equal to 19 and less than 12 mm.
Soussy
C J
CJ
Service de Bactériologie, CHU Henri Mondor, Creteil.
Duval
J
J
Chanal
M
M
Sirot
D
D
Bergogne-Bérézin
E
E
Joly-Guillou
M L
ML
Malbruny
B
B
Morel
C
C
D000150:Acinetobacter / Q000187:drug effects; D000667:Ampicillin / Q000031:analogs & derivatives* / Q000494:pharmacology; D000668:Ampicillin Resistance; D001420:Bacteria, Aerobic / Q000187:drug effects* / Q000201:enzymology / Q000302:isolation & purification; D004755:Enterobacteriaceae / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D008826:Microbial Sensitivity Tests; D009287:Naphthyridines; D010405:Penicillinase / Q000096:biosynthesis; D011549:Pseudomonas / Q000187:drug effects; D012044:Regression Analysis; D013210:Staphylococcus / Q000187:drug effects
D009287:Naphthyridines; C012122:apalcillin; D000667:Ampicillin; D010405:Penicillinase
Activité antibactérienne in vitro de l'apalcilline sur les bactéries aérobies et courbe de concordance.