title: Growth-promoting activity of tuna growth hormone and expression of tuna growth hormone cDNA in Escherichia coli.
abstract: Tuna (Thunnus thynnus) growth hormone (GH) was purified by using a column of Sepharose 4B to which tuna GH-specific IgG was linked. The molecular weight and isoelectric point of tuna GH were 21,000 and 6.5, respectively. The growth of snapper (Pagrus major) was remarkably accelerated when the purified hormone was administered by four intraperitonial injections at intervals of 5 days: 1.5-fold in length and 1.9-fold in body weight/60 days. To produce tuna GH in Escherichia coli cells, expression plasmids pTES8 and pTES8S for tuna GH cDNA with or without the signal peptide region were constructed and GH production in E. coli cells was examined with the Maxicell system. The product specified by the plasmids in E. coli cells was immunologically identified to be tuna GH.
authors.affiliation: Institute for Research and Development, Taiyo Fisheries Co., Ltd., Tokyo, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Sato
authors.forename: K
authors.initials: K
authors.lastname: Murata
authors.forename: K
authors.initials: K
authors.lastname: Watanabe
authors.forename: T
authors.initials: T
authors.lastname: Hayami
authors.forename: Y
authors.initials: Y
authors.lastname: Kariya
authors.forename: M
authors.initials: M
authors.lastname: Sakaguchi
authors.forename: S
authors.initials: S
authors.lastname: Kimura
authors.forename: M
authors.initials: M
authors.lastname: Nonaka
authors.forename: A
authors.initials: A
authors.lastname: Kimura
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001835:Body Weight / Q000187:drug effects; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D005399:Fishes / Q000254:growth & development*; D005796:Genes; D013006:Growth Hormone / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D014158:Transcription, Genetic; D014413:Tuna
chemical_list: D013006:Growth Hormone; D004247:DNA