title: Mechanisms of mRNA decay in bacteria: a perspective.
abstract: Messenger RNA decay plays an important role in prokaryotic gene expression. The disparate stabilities of bacterial messages in vivo are a consequence of their differential susceptibility to degradation by cellular endoribonucleases and 3' -exoribonucleases, which in turn results from differences in mRNA sequence and structure. RNase II and polynucleotide phosphorylase, the major bacterial exonucleases involved in mRNA turnover, rapidly degrade single-stranded RNA from the 3' end, but are impeded by 3' stem-loop structures. At present, the identify and substrate specificity of the endonucleases that control mRNA decay rates are relatively poorly defined. Ribosomes and antisense RNA also can influence the stability of transcripts with which they associate. Differences in mRNA stability can contribute to differential expression of genes within polycistronic operons and to modulation of gene expression in response to changes in bacterial growth conditions.
authors.affiliation: Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA.
authors.forename: J G
authors.initials: JG
authors.lastname: Belasco
authors.forename: C F
authors.initials: CF
authors.lastname: Higgins
mesh_terms: D001419:Bacteria / Q000235:genetics*; D005786:Gene Expression Regulation; D005798:Genes, Bacterial; D012333:RNA, Messenger / Q000235:genetics*; D012270:Ribosomes / Q000378:metabolism
chemical_list: D012333:RNA, Messenger