Location of the Bacillus subtilis temperate bacteriophage phi 105 attP attachment site.
Chromosomal DNAs of lysogens of phi 105 and phi 105 DI:1t were digested with restriction enzymes EcoRI and HpaI and were probed with nick-translated mature phi 105 DNA. Altered bacteriophage-specific bands in the lysogens were detected, indicating that the phage integrates into the host chromosome at a single site, probably via a Campbell-type circular intermediate. The phage attachment site is centrally located in the phage genome and lies between the phage immunity region and the nonessential deletable region of phi 105.
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D001412:Bacillus subtilis / Q000235:genetics*; D001435:Bacteriophages / Q000235:genetics*; D002874:Chromosome Mapping; D004269:DNA, Bacterial / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics*; D005814:Genes, Viral; D008242:Lysogeny; D011995:Recombination, Genetic
D004269:DNA, Bacterial; D004279:DNA, Viral