Comparison between tuberculoproteins A and C in the serodiagnosis of tuberculosis by enzyme-linked immunosorbent assay.
Tuberculoproteins A and C obtained from culture filtrate of Mycobacterium tuberculosis by alcohol fractionation were utilized in an enzyme-linked immunosorbent assay to measure the amount of reactive IgG in the sera from advanced and moderately advanced tuberculosis patients, leprosy patients and normal individuals. The A-protein detected significant levels of IgG in the sera from both advanced and moderately-advanced tuberculosis patients and patients with leprosy as compared to the normal group. Employing the C-protein, only sera from the advanced tuberculosis patients group gave significantly positive results when compared with the normal control group. The data show that the A-protein is more potent than the C-protein and has a potential serodiagnostic value.
Affronti
L F
LF
Taylor
R
R
Hassan
E M
EM
D001426:Bacterial Proteins / Q000032:analysis* / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000032:analysis; D009904:Optical Rotation; D012698:Serologic Tests; D014376:Tuberculosis / Q000175:diagnosis*
D001426:Bacterial Proteins; C030960:tuberculoprotein, Mycobacterium tuberculosis