Cloning and characterization of an AAC(6') gene from Serratia marcescens.
A gene encoding AAC(6') mediated resistance to netilmicin and amikacin was cloned from the chromosome of a nosocomial strain of Serratia marcescens into the BamHI site of pACYC184. The resistance determinant was localized to a sequence of approximately 1 kb. A 490 base pair BamHI-PvuII restriction fragment from the cloned DNA was used as a probe in Southern hybridization studies. Homology with a 920 base pair fragment of PvuII digested S. marcescens DNA from AAC(6')-producing strains was observed. No homology was detected with aminoglycoside sensitive S. marcescens or Escherichia coli.
Champion
H M
HM
Department of Medical Microbiology, Southmead Hospital, Westbury-on-Trym, Bristol, UK.
Bennett
P M
PM
Lewis
D A
DA
Reeves
D S
DS
D000123:Acetyltransferases / Q000378:metabolism; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D004352:Drug Resistance, Microbial / Q000235:genetics; D005798:Genes, Bacterial; D009428:Netilmicin / Q000494:pharmacology; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D015183:Restriction Mapping; D012706:Serratia marcescens / Q000235:genetics*
D009428:Netilmicin; D004247:DNA; D000123:Acetyltransferases; C019681:aminoglycoside acetyltransferase