The irreversibility of thiamin transport in Saccharomyces cerevisiae.
Neither exit nor counterflow efflux of thiamin, taken up previously by an active transport, were found in Saccharomyces cerevisiae, in either the wild type or a mutant with a lower rate of thiamin phosphorylation. Complete inhibition of thiamin phosphorylation by oxythiamin did not lead to any release of thiamin taken up by the cell.
Ruml
T
T
Department of Biochemistry and Microbiology, Institute of Chemical Technology, Prague.
Silhánková
L
L
Rauch
P
P
D001693:Biological Transport, Active / Q000187:drug effects; D007700:Kinetics; D009154:Mutation; D010119:Oxythiamine / Q000494:pharmacology; D010766:Phosphorylation; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D013831:Thiamine / Q000037:antagonists & inhibitors / Q000378:metabolism*
D010119:Oxythiamine; D013831:Thiamine