title: The effect of high glucose and high insulin concentrations on pentose phosphate shunt enzymes and malic enzyme in cultured human endothelial cells.
abstract: The influence of glucose and insulin on pentose phosphate shunt enzymes and malic enzyme activity in cultured human endothelial cells has been investigated. Glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase and malic enzyme were present in endothelial cells. Enzyme activities were not altered either by 20 mM glucose or 10(-8) M insulin after 3, 6 and 12 hour incubations respectively. Neither increased glucose nor increased insulin alter the activity of the pentose phosphate shunt. As a consequence fatty acid and cholesterol synthesis in the endothelial cell is unlikely to be altered in the presence of increased glucose or increased insulin.
authors.affiliation: Department of Medicine, Medical School, Newcastle upon Tyne, United Kingdom.
authors.forename: G C
authors.initials: GC
authors.lastname: Hawthorne
authors.forename: K G
authors.initials: KG
authors.lastname: Alberti
mesh_terms: D004730:Endothelium, Vascular / Q000201:enzymology*; D005947:Glucose / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D007328:Insulin / Q000494:pharmacology*; D008291:Malate Dehydrogenase / Q000378:metabolism*; D010427:Pentose Phosphate Pathway / Q000187:drug effects*
chemical_list: D007328:Insulin; D008291:Malate Dehydrogenase; D005947:Glucose