Specific and sensitive immunological diagnosis of Chagas' disease by competitive antibody enzyme immunoassay using a Trypanosoma cruzi-specific monoclonal antibody.
Coexistence of Chagas' disease with leishmaniasis and T. rangeli infection in endemic areas and cross-reactivity between corresponding etiological agents can confuse the immunodiagnosis of Chagas' disease. A discriminative serological test could therefore represent a major advance in specific immunodiagnosis. A competitive antibody enzyme immunoassay against a component 5-enriched preparation, using a T. cruzi species-specific monoclonal antibody has allowed development of a specific serodiagnosis of Chagas' disease with high sensitivity (96.6% in undetermined and chronic phases of infection). This test can differentiate Chagas' disease from other cross-reacting parasitic diseases in areas where concomitant infections are unknown or suspected.
Lemesre
J L
JL
Afchain
D
D
Orozco
O
O
Loyens
M
M
Breniere
F S
FS
Desjeux
P
P
Carlier
Y
Y
Martin
U
U
Nogueira-Queiroz
J A
JA
Le Ray
D
D
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D000953:Antigens, Protozoan / Q000032:analysis / Q000276:immunology*; D014355:Chagas Disease / Q000175:diagnosis*; D006801:Humans; D007124:Immunoenzyme Techniques; D008970:Molecular Weight; D012698:Serologic Tests; D014349:Trypanosoma cruzi / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan