title: Connective tissue activation. XXX: Isoelectric point microheterogeneity of CTAP-III, a human platelet-derived growth factor.
abstract: Connective tissue activating peptide (CTAP-III) is one of the growth factors found in the alpha granules of human platelets. This small cationic platelet protein shows several apparent isoelectric point variants after the preparative isoelectric focusing step in large scale preparations from multiple donors. Analytical isoelectricfocusing techniques under denaturing conditions, coupled with Western blotting and immunodetection, now show that CTAP-III prepared rapidly from single donors also has multiple isoelectric point variants, suggesting that this finding is more likely related to post-translational modification than preparative artifact or genetic polymorphism.
authors.forename: M S
authors.initials: MS
authors.lastname: Green
authors.forename: P A
authors.initials: PA
authors.lastname: Hossler
authors.forename: C W
authors.initials: CW
authors.lastname: Castor
mesh_terms: D001792:Blood Platelets / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D006133:Growth Substances / Q000032:analysis*; D006801:Humans; D005779:Immunodiffusion; D007526:Isoelectric Point; D010455:Peptides / Q000032:analysis*; D010982:Platelet-Derived Growth Factor / Q000032:analysis*
chemical_list: D006133:Growth Substances; D010455:Peptides; D010982:Platelet-Derived Growth Factor; C009862:connective tissue-activating peptide