Effect of DNA sequence and structure on nuclease activity of the DexA protein of bacteriophage T4.
The bacteriophage T4 dexA gene product is required during infection of Escherichia coli strains carrying a mutation in the optA gene. We purified the DexA protein from cells which overproduced the protein. The protein was assayed for nuclease activity on synthetic di- and oligonucleotide substrates of known sequence and secondary structure. Sequence and structure significantly affected nuclease activity. The properties of the enzyme may explain the requirement for the DexA protein during infection of optA mutant hosts.
Gruber
H
H
Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder 80309.
Kern
G
G
Gauss
P
P
Gold
L
L
D001483:Base Sequence; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D043211:Exodeoxyribonuclease V; D005090:Exodeoxyribonucleases / Q000378:metabolism*; D005796:Genes; D005814:Genes, Viral; D007700:Kinetics; D008969:Molecular Sequence Data; D009154:Mutation; D015345:Oligonucleotide Probes; D013604:T-Phages / Q000201:enzymology / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism
D015345:Oligonucleotide Probes; D014764:Viral Proteins; C052212:dexA protein, T4 phage; D005090:Exodeoxyribonucleases; D043211:Exodeoxyribonuclease V