Biosynthesis of the wall acidic polysaccharide in Bacillus cereus AHU 1356.
Biosynthetic studies on an acidic polysaccharide, comprising galactose, rhamnose, N-acetylglucosamine and sn-glycerol 1-phosphate, were carried out with a membrane system obtained from Bacillus cereus AHU 1356. Incubation of the membranes with UDP-[14C]Gal, TDP-[14C]Rha and UDP-[14C]GlcNAc resulted in the formation of four or more labeled-sugar-linked lipids and a labeled polysaccharide. Data on structural analysis of the sugar moieties released from the glycolipids, together with results of enzymatic conversion of [14C]galactose-linked lipid and [14C]Rha-Gal-linked lipid to higher-oligosaccharide-linked lipids and polysaccharide, led to the conclusion that the acidic polysaccharide is probably synthesized through the following pathway: (sequence in text) The glycerophosphate residues seem to be derived from phosphatidylglycerol.
Kojima
N
N
Araki
Y
Y
Ito
E
E
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001409:Bacillus cereus / Q000378:metabolism*; D001704:Biopolymers; D002473:Cell Wall / Q000378:metabolism; D002852:Chromatography, Ion Exchange; D002854:Chromatography, Paper; D005690:Galactose / Q000378:metabolism; D005994:Glycerophosphates / Q000378:metabolism; D006017:Glycolipids / Q000096:biosynthesis; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009005:Monosaccharides / Q000378:metabolism; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis*; D014415:Tunicamycin / Q000031:analogs & derivatives
D000900:Anti-Bacterial Agents; D001704:Biopolymers; D005994:Glycerophosphates; D006017:Glycolipids; D009005:Monosaccharides; D011135:Polysaccharides, Bacterial; D014415:Tunicamycin; C017012:antibiotic 24010; D005690:Galactose