title: Specific and sensitive immunological diagnosis of Chagas' disease by competitive antibody enzyme immunoassay using a Trypanosoma cruzi-specific monoclonal antibody.
abstract: Coexistence of Chagas' disease with leishmaniasis and T. rangeli infection in endemic areas and cross-reactivity between corresponding etiological agents can confuse the immunodiagnosis of Chagas' disease. A discriminative serological test could therefore represent a major advance in specific immunodiagnosis. A competitive antibody enzyme immunoassay against a component 5-enriched preparation, using a T. cruzi species-specific monoclonal antibody has allowed development of a specific serodiagnosis of Chagas' disease with high sensitivity (96.6% in undetermined and chronic phases of infection). This test can differentiate Chagas' disease from other cross-reacting parasitic diseases in areas where concomitant infections are unknown or suspected.
authors.forename: J L
authors.initials: JL
authors.lastname: Lemesre
authors.forename: D
authors.initials: D
authors.lastname: Afchain
authors.forename: O
authors.initials: O
authors.lastname: Orozco
authors.forename: M
authors.initials: M
authors.lastname: Loyens
authors.forename: F S
authors.initials: FS
authors.lastname: Breniere
authors.forename: P
authors.initials: P
authors.lastname: Desjeux
authors.forename: Y
authors.initials: Y
authors.lastname: Carlier
authors.forename: U
authors.initials: U
authors.lastname: Martin
authors.forename: J A
authors.initials: JA
authors.lastname: Nogueira-Queiroz
authors.forename: D
authors.initials: D
authors.lastname: Le Ray
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D000953:Antigens, Protozoan / Q000032:analysis / Q000276:immunology*; D014355:Chagas Disease / Q000175:diagnosis*; D006801:Humans; D007124:Immunoenzyme Techniques; D008970:Molecular Weight; D012698:Serologic Tests; D014349:Trypanosoma cruzi / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan