A simple device to aid tissue processing of large numbers of blocks for light or electron microscopy.
A simple device is described that aids the processing of large numbers of tissue blocks for light or electron microscopy. The device has a numbers of compartments into which different tissue blocks can be placed. Each compartment has a fine mesh floor, allowing the free exchange of the reagents used in tissue processing, and a gentle flow of the reagents is promoted by a magnetic stirring bar, or a rotary mixer. All the materials used are resistant to solvents such as propylene oxide. The device is labor-saving in that many blocks can be processed as easily as a single block. The device may also be of value in quantitative microscopy, since it ensures that all tissue blocks examined experience an identical processing history.
Smith
K J
KJ
Department of Anatomy and Cell Biology, Eastern Virginia Medical School, Norfolk 23501.
Larramendi
P C
PC
D006652:Histological Techniques; D008853:Microscopy / Q000379:methods*; D008854:Microscopy, Electron / Q000379:methods