Immunoglobulin G in multiple sclerosis brain.
We extracted free and bound IgG from plaques and normal-appearing white matter of multiple sclerosis (MS) brains. By isoelectric focusing (IEF), three patterns of IgG distribution were seen: (A) a restricted high-pI distribution with a specific band at low pH, (B) a restricted high-pI pattern, and (C) a broad pI pattern similar to that of the unbound IgG extracted at neutral pH. In one MS brain, we compared the IEF pattern of plaque material with that of normal-appearing white matter (NAWM); the low-pH extract of plaque material (PM) had a restricted pattern at high pI. In another MS brain, a specific band of bound IgG was found. These data suggest that MS lesions expose an antigen(s) unique to MS. B cells consequently might be stimulated by a disease-related antigen(s) in the MS lesion.
Hosokawa
T
T
Department of Neurology, Saitama Medical School, Japan.
Hunt
J C
JC
Marchalonis
J J
JJ
Hogan
E L
EL
D001921:Brain / Q000276:immunology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000032:analysis*; D007525:Isoelectric Focusing; D009103:Multiple Sclerosis / Q000276:immunology*
D007074:Immunoglobulin G