title: Expression of the staphylococcal protein A gene in Bacillus subtilis by integration of the intact gene into the B. subtilis chromosome.
abstract: Staphylococcal protein A was synthesized at high levels and was secreted efficiently into the culture medium by strains of Bacillus subtilis in which the cloned gene (spa) from Staphylococcus aureus 8325-4 was inserted into the chromosome. The spa gene could not be established in B. subtilis on multicopy plasmids.
authors.forename: S R
authors.initials: SR
authors.lastname: Fahnestock
authors.forename: C W
authors.initials: CW
authors.lastname: Saunders
authors.forename: M S
authors.initials: MS
authors.lastname: Guyer
authors.forename: S
authors.initials: S
authors.lastname: Löfdahl
authors.forename: B
authors.initials: B
authors.lastname: Guss
authors.forename: M
authors.initials: M
authors.lastname: Uhlén
authors.forename: M
authors.initials: M
authors.lastname: Lindberg
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics* / Q000378:metabolism; D002876:Chromosomes, Bacterial; D003001:Cloning, Molecular; D005796:Genes; D005798:Genes, Bacterial; D010957:Plasmids; D011995:Recombination, Genetic; D013205:Staphylococcal Protein A / Q000096:biosynthesis / Q000235:genetics*; D014169:Transformation, Bacterial
chemical_list: D013205:Staphylococcal Protein A