Higher level expression of lymphocyte function-associated antigen-1 (LFA-1) on in vivo natural killer cells.
Normal mouse spleen cells express low levels of lymphocyte function-associated antigen-1 (LFA-1) as well as other lymphoid cells. However, fractionation of spleen cells with Percoll discontinuous gradients resulted in the appearance of lymphocytes expressing high levels of LFA-1 molecule (LFA-1 high lymphocytes) in parallel with the enrichment of natural killer (NK) activity. Lower density spleen cells (fractions 1 and 2) expressed higher level of LFA-1 antigen than unfractionated spleen cells and showed a higher NK activity. In contrast, higher density spleen cells (fractions 3 and 4) expressed lower levels of LFA-1 antigen and revealed lower NK activity. LFA-1 high lymphocytes possessed a high level of asialo GM1, which was the cell surface marker for NK cells. Moreover, sorting of LFA-1 high lymphocytes from spleen cells caused a great enrichment of NK cells. These results demonstrated that in vivo NK cells expressed higher levels of LFA-1 molecule, which was an important adhesion molecule for NK cell-mediated cytotoxicity.
Nishimura
T
T
Department of Anatomy, Tohoku University School of Medicine, Sendai, Japan.
Itoh
T
T
D000818:Animals; D000943:Antigens, Differentiation / Q000276:immunology*; D003602:Cytotoxicity, Immunologic; D005434:Flow Cytometry; D005677:G(M1) Ganglioside; D006028:Glycosphingolipids / Q000032:analysis; D007113:Immunity, Innate; D007694:Killer Cells, Natural / Q000276:immunology*; D016169:Lymphocyte Function-Associated Antigen-1; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H
D000943:Antigens, Differentiation; D006028:Glycosphingolipids; D016169:Lymphocyte Function-Associated Antigen-1; D005677:G(M1) Ganglioside; C018835:asialo GM1 ganglioside