Complementation and hybridization evidence for additional families of type I DNA restriction and modification genes in Salmonella serotypes.
Of eight Salmonella, serB-linked hsd genes for the restriction and modification of DNA transferred to Escherichia coli/Salmonella hybrids, only two--those with SM and ST (S. muenchen and S. thompson, respectively) specificities--may have weakly complemented rSB- and none complemented rK-. An A-specific DNA probe failed to hybridize to HindIII-restricted fragments of each of the hybrids, but an SB (S. typhimurium)-specific probe hybridized to DNA from the hybrid with ST specificity. These results indicate that additional families of the type I hsd genes may exist.
Ryu
J
J
Department of Microbiology, Loma Linda University, California 92350.
Rajadas
P T
PT
Bullas
L R
LR
D004269:DNA, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D012486:Salmonella typhimurium / Q000235:genetics*
D004269:DNA, Bacterial