[Methods of isolating and selecting recombinant vaccinia viruses expressing heterogenetic viral antigens].
The paper describes a method using plasmid construction pSC11 for generation of recombinant vaccinia viruses supporting coexpression of heterologous genes and beta-galactosidase. The Ca2+-phosphate method of cell transfection by recombinant DNAs generated on the basis of pSC11, and selection of recombinant viruses from blue plaques of virus-infected cells in the presence of X-gala are reported at length.
Gibadulin
R A
RA
Iuferov
V P
VP
Lideman
L F
LF
Parasiuk
N A
NA
Ionova
K S
KS
D015478:Antigens, Heterophile / Q000235:genetics*; D000956:Antigens, Viral / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics; D005786:Gene Expression Regulation; D005814:Genes, Viral; D005821:Genetic Techniques; D006389:Hemagglutinins, Viral / Q000235:genetics; D010957:Plasmids; D011995:Recombination, Genetic; D012641:Selection, Genetic; D014162:Transfection; D014616:Vaccinia virus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*
D015478:Antigens, Heterophile; D000956:Antigens, Viral; D004279:DNA, Viral; D006389:Hemagglutinins, Viral
Metody polucheniia i selektsii rekombinantov virusov ospovaktsiny, ékspressiruiushchikh chuzherodnye virusnye antigeny.