A chromosomal mutation mediating increased expression of pyrE in Salmonella typhimurium is located within the proposed attenuator.
A chromosomal mutation resulting in 15- to 20-fold increased expression of the Salmonella typhimurium pyrE gene has been cloned and sequenced. The mutation was found to be a single base-pair transition of GC to AT, and occurred within a region of dyad symmetry (attenuator) located just upstream of the pyrE structural gene.
Neuhard
J
J
Enzyme Division, University Institute of Biological Chemistry B, Copenhagen K, Denmark.
Kelln
R A
RA
D001483:Base Sequence; D002876:Chromosomes, Bacterial; D003001:Cloning, Molecular; D005786:Gene Expression Regulation; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D009154:Mutation; D009962:Orotate Phosphoribosyltransferase / Q000096:biosynthesis / Q000235:genetics*; D010430:Pentosyltransferases / Q000235:genetics*; D010957:Plasmids; D012486:Salmonella typhimurium / Q000201:enzymology / Q000235:genetics*
D010430:Pentosyltransferases; D009962:Orotate Phosphoribosyltransferase