title: Effect of glucose and long-chain fatty acids on synthesis of long-chain alcohols by Candida albicans.
abstract: Candida albicans, grown aerobically in glucose-containing media, produced C14, C16 and C18 saturated long-chain alcohols only after the end of exponential growth. Contents of C14 alcohols were always lowest, and C16 and C18 alcohol contents about equal. Contents of all three classes of alcohol increased as the concentration of glucose in aerobic cultures harvested after 168 h incubation was raised from 1.0 to 30.0% (w/v). However, in 168 h anaerobic cultures, greatest long-chain alcohol contents in organisms were obtained using media containing 10% (w/v) glucose. Substituting glucose (10%, w/v) with the same concentration of galactose in aerobic cultures greatly decreased contents of long-chain alcohols, while inclusion of 10% (w/v) glycerol virtually abolished their synthesis. Supplementing anaerobic cultures with odd-chain fatty acids induced synthesis of odd-chain alcohols. Maximum conversion of fatty acid to the corresponding long-chain alcohol was observed with heptadecanoic acid. The effect of glucose on production of heptadecanol from exogenously provided heptadecanoic acid was similar to that observed on synthesis of the three major even-chain alcohols in media lacking a fatty-acid supplement. Cell-free extracts of organisms catalysed in vitro conversion of palmitoyl-CoA to 1-hexadecanol.
authors.affiliation: Zymology Laboratory, School of Biological Sciences, Bath University, UK.
authors.forename: M J
authors.initials: MJ
authors.lastname: White
authors.forename: R C
authors.initials: RC
authors.lastname: Hammond
authors.forename: A H
authors.initials: AH
authors.lastname: Rose
mesh_terms: D000438:Alcohols / Q000096:biosynthesis*; D000693:Anaerobiosis; D002176:Candida albicans / Q000187:drug effects / Q000378:metabolism*; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology*; D005947:Glucose / Q000494:pharmacology*; D010084:Oxidation-Reduction; D011108:Polymers / Q000494:pharmacology
chemical_list: D000438:Alcohols; D005231:Fatty Acids, Unsaturated; D011108:Polymers; D005947:Glucose