title: Bacterial agglutination and polyacrylamide gel electrophoresis for typing Clostridium difficile.
abstract: Bacterial agglutination and polyacrylamide gel electrophoresis (PAGE) were methods evaluated for typing strains of Clostridium difficile. A panel of four antisera, obtained by immunizing rabbits with washed whole cells of different strains of C. difficile, produced distinctive patterns of agglutination. Ethylenediaminetetraacetate (EDTA) extracts subjected to PAGE also produced distinctive protein profiles. Excellent correlation between the two methods was observed when geographically distant isolates were typed without knowledge of their clinical origin. Both typing methods should receive further evaluation for their value as tools for epidemiological studies.
authors.forename: M E
authors.initials: ME
authors.lastname: Mulligan
authors.forename: S
authors.initials: S
authors.lastname: Halebian
authors.forename: R Y
authors.initials: RY
authors.lastname: Kwok
authors.forename: W C
authors.initials: WC
authors.lastname: Cheng
authors.forename: S M
authors.initials: SM
authors.lastname: Finegold
authors.forename: C R
authors.initials: CR
authors.lastname: Anselmo
authors.forename: D N
authors.initials: DN
authors.lastname: Gerding
authors.forename: L R
authors.initials: LR
authors.lastname: Peterson
mesh_terms: D000372:Agglutination Tests; D001426:Bacterial Proteins / Q000032:analysis; D003013:Clostridium / Q000032:analysis / Q000145:classification* / Q000276:immunology; D003015:Clostridium Infections / Q000382:microbiology; D004492:Edetic Acid; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D012703:Serotyping
chemical_list: D001426:Bacterial Proteins; D004492:Edetic Acid