Screening for skin-tumor promoters.
Since attention has been drawn to the role of oxygen radicals in tumor promotion, we investigated whether or not the nitroblue tetrazolium (NBT) reduction test using mouse peritoneal macrophages was useful as a rapid screening method for skin-tumor promoters. On the basis of our results, we concluded that this test system was appropriate for the screening of 12-0-tetradecanoylphorbol-13-acetate (TPA)-type tumor promoters and bore comparison with other screening methods for tumor promoters. A large number of skin-tumor promoters, such as active phorbol esters, teleocidin and aplysiatoxin, appear to act indirectly through a free radical mechanism by binding to a membrane receptor.
Ohkawa
Y
Y
Department of Cell Biology and Cytogenetics, Japan Tobacco Inc., Kanagawa.
Iwata
K
K
Inui
N
N
D000818:Animals; D015197:Carcinogenicity Tests; D005609:Free Radicals; D051379:Mice; D009580:Nitroblue Tetrazolium / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism; D012878:Skin Neoplasms / Q000139:chemically induced*; D013755:Tetradecanoylphorbol Acetate; D013778:Tetrazolium Salts / Q000378:metabolism*
D005609:Free Radicals; D013778:Tetrazolium Salts; D009580:Nitroblue Tetrazolium; D013755:Tetradecanoylphorbol Acetate; D010100:Oxygen