title: [Effect of ionizing radiation on the structure of human serum albumin. Effect of irradiation on the content of free amino groups and on the ability of HSA to be digested by proteinases].
abstract: The effect of gamma-quanta on human serum albumin (HSA) solutions (1.6 mg/ml, borate buffer pH 7.45) in the air has been investigated. Using 2,4,6-trinitro-benzene-sulfonic acid it has been shown that 2-22 kGy radiation reduces the free amino groups content of HSA and increases its resistance to the hydrolytic effect of trypsin and pronase which is not influenced by the postirradiation exposure to heat. It is concluded that epsilon-NH2-groups of lysine residues are modified and firm cross-links are formed in HSA under the effect of radiation.
authors.forename: N V
authors.initials: NV
authors.lastname: Kondakova
authors.forename: N V
authors.initials: NV
authors.lastname: Ripa
authors.forename: V V
authors.initials: VV
authors.lastname: Sakharova
mesh_terms: D003037:Cobalt Radioisotopes; D005720:Gamma Rays; D006801:Humans; D066298:In Vitro Techniques; D015394:Molecular Structure; D010447:Peptide Hydrolases / Q000378:metabolism*; D011402:Pronase / Q000378:metabolism; D012709:Serum Albumin / Q000528:radiation effects*; D014357:Trypsin / Q000378:metabolism
chemical_list: D003037:Cobalt Radioisotopes; D012709:Serum Albumin; D010447:Peptide Hydrolases; D014357:Trypsin; D011402:Pronase
vernacular_title: Deĭstvie ioniziruiushcheĭ radiatsii na strukturu syvorotochnogo al'bumina cheloveka. Vliianie oblucheniia na soderzhanie svobodnykh aminogrupp i sposobnost' SACh perevarivat'sia proteinazami.