Compartment analysis of ATP in malaria-infected erythrocytes.
The ATP concentration of malaria-infected erythrocytes changes substantially with parasite development. These alterations have been attributed to a decline in host cell [ATP], but have not been tested critically hitherto. A method for the compartmental analysis of ATP in malaria (Plasmodium falciparum)-infected human red blood cells has been developed using Sendai virus to permeabilize the host erythrocyte membrane. Permeabilization and release of host cytosol was complete within 6 to 8 minutes and ATP was measured by the luciferin-luciferase bioluminescence assay in the lysate and in the pellet. Equal ATP concentrations were found in host and parasite compartments at the trophozoite and schizont stages. Both were lower than those detected in uninfected cells. Other methods for compartment analysis of ATP are presented and discussed.
Kanaani
J
J
Department of Biological Chemistry, Hebrew University of Jerusalem, Israel.
Ginsburg
H
H
D000255:Adenosine Triphosphate / Q000097:blood*; D000818:Animals; D002451:Cell Compartmentation; D002463:Cell Membrane Permeability; D003600:Cytosol / Q000378:metabolism; D004912:Erythrocytes / Q000378:metabolism* / Q000469:parasitology / Q000648:ultrastructure; D006801:Humans; D066298:In Vitro Techniques; D008288:Malaria / Q000097:blood*; D010963:Plasmodium falciparum
D000255:Adenosine Triphosphate