title: Construction of a plasmid for high-level expression of goat alpha-lactalbumin and its derivative in E. coli.
abstract: The high-level expression system of goat alpha-lactalbumin (alpha-LA) in E. coli was established by fusing the alpha-LA cDNA to porcine adenylate kinase cDNA and expressing the fused gene under the control of tac promoter. For high-level expression, elimination of 3'-noncoding region of the alpha-LA cDNA was found to be necessary.
authors.affiliation: Department of Industrial Chemistry, Faculty of Engineering, University of Tokyo, Japan.
authors.forename: I
authors.initials: I
authors.lastname: Kumagai
authors.forename: M
authors.initials: M
authors.lastname: Inaizumi
authors.forename: E
authors.initials: E
authors.lastname: Tamaki
authors.forename: S
authors.initials: S
authors.lastname: Misawa
authors.forename: T
authors.initials: T
authors.lastname: Hibino
authors.forename: K
authors.initials: K
authors.lastname: Miura
mesh_terms: D000818:Animals; D003001:Cloning, Molecular / Q000379:methods; D004926:Escherichia coli / Q000235:genetics*; D005796:Genes; D006041:Goats; D007768:Lactalbumin / Q000235:genetics*; D010957:Plasmids
chemical_list: D007768:Lactalbumin