A novel two colour ELISPOT assay. I. Simultaneous detection of distinct types of antibody-secreting cells.
A novel assay system has been developed which is based on the ELISPOT methodology and employs a combination of two immunoenzyme visualization systems yielding distinct colour products. This variation permits the simultaneous enumeration of two different types of cell secreting antigenically distinct products. Optimal conditions for the concurrent detection of human mononuclear cells secreting IgG or IgA antibodies are described.
Czerkinsky
C
C
Department of Microbiology, University of Alabama, Birmingham.
Moldoveanu
Z
Z
Mestecky
J
J
Nilsson
L A
LA
Ouchterlony
O
O
D000328:Adult; D000914:Antibodies, Viral / Q000096:biosynthesis; D000921:Antibody-Producing Cells / Q000032:analysis*; D002863:Chromogenic Compounds; D004797:Enzyme-Linked Immunosorbent Assay; D006462:Hemolytic Plaque Technique; D006801:Humans; D007070:Immunoglobulin A / Q000096:biosynthesis; D007074:Immunoglobulin G / Q000096:biosynthesis; D007202:Indicators and Reagents; D009980:Influenza A virus / Q000276:immunology
D000914:Antibodies, Viral; D002863:Chromogenic Compounds; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007202:Indicators and Reagents