Immunoreactive dopamine beta-hydroxylase in neuronal groups in the goldfish brain.
Dopamine beta-hydroxylase immunoreactivity is visualized by immunocytochemical techniques for the first time in brainstem neurons of a teleost, the goldfish, Carassius auratus. Three anatomically separate populations of immunoreactive cells are elucidated: one group of moderately stained cells is identified in the isthmal tegmentum; a second group forms a continuous column of densely stained cells, lateral or dorsolateral to the medial longitudinal fasciculus from the midmedulla to the medullospinal transition zone, and a third group of densely stained cells is located dorsomedially in the postobecular region of the medulla. The location of these dopamine-beta-hydroxylase-immunoreactive neurons is similar to that in mammalian brains, i.e. in the locus coeruleus, lateral medullary tegmentum and area postrema. The presence of norepinephrine-synthesizing neurons in the above-listed brainstem regions of the goldfish provides further evidence for homologizing these nuclei to the same-named regions in mammalian brains.
Hornby
P J
PJ
Neuroendocrine Unit, University of Rochester School of Medicine and Dentistry, N.Y.
Piekut
D T
DT
D000818:Animals; D001921:Brain / Q000033:anatomy & histology*; D001931:Brain Mapping; D003530:Cyprinidae / Q000033:anatomy & histology*; D004299:Dopamine beta-Hydroxylase / Q000032:analysis*; D006054:Goldfish / Q000033:anatomy & histology*; D007124:Immunoenzyme Techniques; D008125:Locus Coeruleus / Q000033:anatomy & histology; D009474:Neurons / Q000648:ultrastructure; D010625:Phenylethanolamine N-Methyltransferase / Q000032:analysis
D004299:Dopamine beta-Hydroxylase; D010625:Phenylethanolamine N-Methyltransferase