[Ca2+]i in single isolated cardiac cells: a review of recent results obtained with digital imaging microscopy and fura-2.
The use of fluorescent Ca2+ indicators to observe [Ca2+]i transients in voltage-clamped single cells has many advantages over previous methods, such as the use of aequorin in multicellular preparations, for studying excitation-contraction coupling. In the studies reviewed in this article, [Ca2+]i in single isolated mammalian ventricular myocytes was observed through the use of the fluorescent Ca2+ indicator, fura-2. Individual cells, loaded with fura-2 either by internal perfusion or by exposure to fura-2/AM, were generally studied with the use of inverted microscopes equipped with ultraviolet epifluorescence illumination, intensified silicon intensifier target cameras (ISIT), and (or) a photomultiplier tube. Analysis of subcellular patterns of fura-2 fluorescence was performed by digital analysis of the images obtained with the ISIT camera. Variation of membrane voltage and exposure of cells to ryanodine (which was assumed to selectively block the release of Ca2+ from the sarcoplasmic reticulum) were used to investigate the cellular processes that determine the [Ca2+]i transient. The main results of these studies are the following. (1) In any population of enzymatically isolated heart cells, there are (i) mechanically quiescent cells in which [Ca2+]i is spatially uniform, constant over time, and relatively low; (ii) spontaneously contracting cells, which have a relatively elevated [Ca2+]i, but in which the spatial uniformity of [Ca2+]i is interrupted periodically by spontaneous, propagating waves of high [Ca2+]i; and (iii) cells that are hypercontracted (rounded up) and that have higher levels of [Ca2+]i than the other two types.(ABSTRACT TRUNCATED AT 250 WORDS)
Wier
W G
WG
Department of Physiology, University of Maryland, School of Medicine, Baltimore 21204.
Beuckelmann
D J
DJ
Barcenas-Ruiz
L
L
D000818:Animals; D001572:Benzofurans; D002118:Calcium / Q000378:metabolism*; D004594:Electrophysiology; D016257:Fura-2; D006321:Heart / Q000502:physiology; D008564:Membrane Potentials; D008853:Microscopy; D008955:Models, Cardiovascular; D009206:Myocardium / Q000032:analysis*; D012815:Signal Processing, Computer-Assisted
D001572:Benzofurans; D002118:Calcium; D016257:Fura-2