Extraction of plant proteins for two-dimensional electrophoresis.
Three different extraction procedures for two-dimensional electrophoresis of plant proteins are compared: (i) extraction of soluble proteins with a nondenaturing Tris-buffer, (ii) denaturing extraction in presence of sodium dodecyl sulfate at elevated temperature allowing the solubilization of membrane proteins in addition to a recovery of soluble proteins, and (iii) a trichloroacetic acid-acetone procedure allowing the direct precipitation of total proteins.
Granier
F
F
Laboratoire de Génétique des Systèmes Végétaux, La Ferme du Moulon, Gif-sur-Yvette, France.
D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D010447:Peptide Hydrolases / Q000378:metabolism; D010449:Peptide Mapping; D010940:Plant Proteins / Q000302:isolation & purification*; D011480:Protease Inhibitors / Q000494:pharmacology; D011489:Protein Denaturation; D012995:Solubility; D003313:Zea mays / Q000032:analysis
D010940:Plant Proteins; D011480:Protease Inhibitors; D010447:Peptide Hydrolases