title: Chimeric single-stranded DNA phage-plasmid cloning vectors.
abstract: A variety of ssDNA phagemid cloning vectors have been constructed that combine the advantages of the filamentous coliphages with a number of plasmid and other bacteriophage-encoded functions. The practical and biological advantages of a chimeric phage-plasmid vector are considerable, and the trend toward converting existing plasmids into ssDNA phagemids and consolidating any number of useful features into one or a few vectors will undoubtedly accelerate. A new helper phage specifically designed for the isolation of large amounts of single-stranded plasmid DNA simplifies the use of these cloning vehicles. Additional refinements in phagemid-helper phage systems should extend the potential of these vectors even further.
authors.forename: D A
authors.initials: DA
authors.lastname: Mead
authors.forename: B
authors.initials: B
authors.lastname: Kemper
mesh_terms: D001435:Bacteriophages / Q000235:genetics*; D001483:Base Sequence; D004277:DNA, Single-Stranded / Q000235:genetics*; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D010957:Plasmids
chemical_list: D004277:DNA, Single-Stranded