A modified fibrin plate for rapid detection of proteinases and proteinase inhibitors in fractions after liquid chromatography.
A rapid procedure for detecting proteolytic enzymes and proteinase inhibitors in fractions after liquid chromatography has been developed. The detection is based on the proteolysis of a very thin layer of fibrin. Proteinase (or proteinase inhibitor) positive and negative fractions can be distinguished in a short period of time.
Safarík
I
I
Laboratory of Analytical Chemistry, South Bohemian Biological Centre, Budĕjovice, Czechoslovakia.
D002853:Chromatography, Liquid / Q000379:methods; D005337:Fibrin; D005340:Fibrinogen / Q000032:analysis; D007202:Indicators and Reagents; D008832:Microchemistry; D010447:Peptide Hydrolases / Q000032:analysis*; D011480:Protease Inhibitors / Q000032:analysis*
D007202:Indicators and Reagents; D011480:Protease Inhibitors; D005337:Fibrin; D005340:Fibrinogen; D010447:Peptide Hydrolases