title: Plasmodium vivax: cloning and expression of a major blood-stage surface antigen.
abstract: Plasmodium vivax is a highly prevalent malaria pathogen of man; the following report is the first to describe the cloning and expression of a major asexual erythrocytic stage antigen of this species. The screening of a genomic DNA expression library with a monoclonal antibody directed against a 200-kDa surface component (Pv200) of the more mature schizonts of P. vivax led to the selection of a recombinant bacterial clone which produced a fusion protein. Mouse and rabbit immune sera raised against the purified fusion protein recognized the 200-kDa parasite antigen on Western blots and reacted with the surface of segmenters by immunofluorescence. Sequencing of the 1.9-kb P. vivax DNA insert coding for this fusion protein revealed a 45-47% homology at the nucleotide level with the P. falciparum gene of a parasite surface antigen, Pf195, which has been shown to be a promising candidate for a malaria vaccine in primates and in man.
authors.affiliation: Department of Immunology, Institut Pasteur, Paris, France.
authors.forename: H A
authors.initials: HA
authors.lastname: del Portillo
authors.forename: J
authors.initials: J
authors.lastname: Gysin
authors.forename: D M
authors.initials: DM
authors.lastname: Mattei
authors.forename: E
authors.initials: E
authors.lastname: Khouri
authors.forename: P V
authors.initials: PV
authors.lastname: Udagama
authors.forename: K N
authors.initials: KN
authors.lastname: Mendis
authors.forename: P H
authors.initials: PH
authors.lastname: David
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000953:Antigens, Protozoan / Q000096:biosynthesis / Q000235:genetics*; D000954:Antigens, Surface / Q000096:biosynthesis / Q000235:genetics; D001483:Base Sequence; D015153:Blotting, Western; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation; D008969:Molecular Sequence Data; D010966:Plasmodium vivax / Q000235:genetics* / Q000276:immunology
chemical_list: D000953:Antigens, Protozoan; D000954:Antigens, Surface; D004247:DNA