The monoclonal antibody CVI-ChNL-68.1 recognizes cells of the monocyte-macrophage lineage in chickens.
The characteristics of monoclonal antibody CVI-ChNL-68.1, which specifically reacts with a group of chicken non-lymphoid cells, are described. Both tissue distribution shown on cryostat sections using immuno-enzyme histochemistry, and quantitative data obtained on cell suspensions are presented. Functional characteristics of CVI-ChNL-68.1-positive cells, such as antigen uptake and glass adherence, are determined. Results show that CVI-ChNL-68.1 reacts with monocytes, macrophages, and interdigitating cells. Possible relationships between the various non-lymphoid cells are discussed.
Jeurissen
S H
SH
Department of Virology, Central Veterinary Institute, Lelystad, The Netherlands.
Janse
E M
EM
Koch
G
G
de Boer
G F
GF
D000135:Acid Phosphatase / Q000032:analysis; D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D000938:Antigen-Presenting Cells / Q000276:immunology*; D000941:Antigens / Q000032:analysis; D002448:Cell Adhesion; D002645:Chickens / Q000276:immunology*; D007124:Immunoenzyme Techniques; D007801:Langerhans Cells; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009000:Monocytes / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000941:Antigens; D000135:Acid Phosphatase