In situ detection of rubella RNA and antigens in cultured cells.
We developed in situ hybridization and immunofluorescent procedures to detect rubella RNA and antigens in tissue-cultured cells infected with rubella virus. cDNA fragments of the rubella virus E1 structural gene were used as probes for in situ hybridization to detect rubella RNA sequences in Vero cells infected with rubella virus. Using antibodies against rubella proteins, indirect immunofluorescence detected rubella virus structural proteins in Vero cells infected with rubella virus. The immunofluorescence method has also been applied to study the expression of rubella polypeptide E1 in transfected COS cells and may be applied to the detection and study of persistent rubella virus infection in human tissues.
Filipenko
D
D
Department of Pathology, Faculty of Medicine, University of British Columbia Research Centre, Vancouver, Canada.
Hobman
T
T
MacDonald
I
I
Gillam
S
S
D000818:Animals; D000914:Antibodies, Viral; D000956:Antigens, Viral / Q000302:isolation & purification*; D015342:DNA Probes; D005455:Fluorescent Antibody Technique; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000302:isolation & purification*; D012412:Rubella virus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D014709:Vero Cells
D000914:Antibodies, Viral; D000956:Antigens, Viral; D015342:DNA Probes; D012367:RNA, Viral; C040901:rubella antibodies