title: The Escherichia coli biotin biosynthetic enzyme sequences predicted from the nucleotide sequence of the bio operon.
abstract: The nucleotide sequence of the biotin (bio) biosynthetic operon of Escherichia coli has been determined. The 5.8-kilobase region contains the five biotin operon genes, bioA, B, F, C, and D. and an open reading frame of unknown function. The operon is negatively regulated and divergently transcribed from a control region between the bioA and bioB genes. The product of the bioA gene, 7,8-diaminopelargonic acid aminotransferase, was discovered to be related to ornithine aminotransferase. The product of the bioF gene, 7-keto-8-aminopelargonic acid synthetase, was found to be similar to 5-aminolevulinic acid synthetase.
authors.affiliation: Department of Genetics, University of California, Berkeley 94720.
authors.forename: A J
authors.initials: AJ
authors.lastname: Otsuka
authors.forename: M R
authors.initials: MR
authors.lastname: Buoncristiani
authors.forename: P K
authors.initials: PK
authors.lastname: Howard
authors.forename: J
authors.initials: J
authors.lastname: Flamm
authors.forename: C
authors.initials: C
authors.lastname: Johnson
authors.forename: R
authors.initials: R
authors.lastname: Yamamoto
authors.forename: K
authors.initials: K
authors.lastname: Uchida
authors.forename: C
authors.initials: C
authors.lastname: Cook
authors.forename: J
authors.initials: J
authors.lastname: Ruppert
authors.forename: J
authors.initials: J
authors.lastname: Matsuzaki
mesh_terms: D000624:5-Aminolevulinate Synthetase / Q000235:genetics; D000595:Amino Acid Sequence; D001483:Base Sequence; D001710:Biotin / Q000096:biosynthesis*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005796:Genes; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009876:Operon; D009953:Ornithine-Oxo-Acid Transaminase / Q000235:genetics; D011487:Protein Conformation; D014158:Transcription, Genetic
chemical_list: D001710:Biotin; D000624:5-Aminolevulinate Synthetase; D009953:Ornithine-Oxo-Acid Transaminase