Affinity isolation of the interleukin-3 surface receptor.
Interleukin-3 (IL-3) is a pluripotent hematopoietic growth factor which can stimulate both proliferation and differentiation of progenitor cells. Using the factor dependent NSF-60 and FDC-P1 cells, which possess specific, high affinity binding sites for 125I-IL-3 (i.e. KD 9.5 and 10.7 X 10(-11)M, respectively) the IL-3 surface receptor has been isolated. Using two separate affinity techniques the IL-3 receptor identified from both cell types was invariant. The IL-3 receptor is a protein of molecular weight 65-70,000 daltons.
May
W S
WS
Ihle
J N
JN
D000345:Affinity Labels; D000818:Animals; D002460:Cell Line; D003432:Cross-Linking Reagents; D007377:Interleukin-3; D007700:Kinetics; D008222:Lymphokines / Q000378:metabolism*; D008565:Membrane Proteins / Q000302:isolation & purification; D051379:Mice; D008970:Molecular Weight; D011971:Receptors, Immunologic / Q000302:isolation & purification* / Q000378:metabolism; D016185:Receptors, Interleukin-3
D000345:Affinity Labels; D003432:Cross-Linking Reagents; D007377:Interleukin-3; D008222:Lymphokines; D008565:Membrane Proteins; D011971:Receptors, Immunologic; D016185:Receptors, Interleukin-3