Stable preparation of yeast mitochondria and mitoplasts synthesizing specific polypeptides.
Yeast mitochondria isolated in the presence of 0.6 M sorbitol and 0.5% bovine serum albumin can be stored in liquid nitrogen without loss of translational activity. Frozen mitochondria retain the respiratory control and the mutant pattern of polypeptide synthesis identical to those detected for fresh preparations. Stored mitochondria may be efficiently transformed into a stable preparation of mitoplasts actively synthesizing mitochondrial polypeptides.
Kozłowski
M
M
Institute of Biochemistry and Biophysies, Polish Academy of Sciences, Warsaw.
Zagórski
W
W
D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000096:biosynthesis*; D008928:Mitochondria / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism*
D005656:Fungal Proteins