title: [Long-term cultivation of calf aortic endothelial cells. Demonstration of the production of factor VIII antigen].
abstract: Endothelial cells were able to accomplish at least 120 cell doublings (60 passages) without any decrease of division speed. After that, the doubling time begins to increase. At passage 80, the number of cells was not doubled even after a period of 4 weeks. Temperature stress (41 degrees C for 3 d) leads the cells to normal doubling times again and allows the cultures to reach passage 124. The cells were able to produce factor VIII antigen, but in less quantities, still at passage 124.
authors.forename: M
authors.initials: M
authors.lastname: Melzig
authors.forename: E
authors.initials: E
authors.lastname: Teuscher
authors.forename: R
authors.initials: R
authors.lastname: Miethchen
mesh_terms: D000818:Animals; D001011:Aorta; D002455:Cell Division; D002470:Cell Survival; D002478:Cells, Cultured; D004727:Endothelium / Q000378:metabolism*; D005169:Factor VIII / Q000096:biosynthesis*; D006358:Hot Temperature; D013552:Swine; D013997:Time Factors
chemical_list: D005169:Factor VIII
vernacular_title: Untersuchungen zur Langzeitkultivierung von Kälberaortenendothelzellen. Nachweis der Bildung des Faktor-VIII-Antigens.