title: Nucleotide sequence of the ugp genes of Escherichia coli K-12: homology to the maltose system.
abstract: The nucleotide sequence of the ugp genes of Escherichia coli K-12, which encode a phosphate-limitation inducible uptake system for sn-glycerol-3-phosphate and glycerophosphoryl diesters, was determined. The genetic organization of the operon differed from previously published results. A single promoter, containing a putative pho box, was detected by S1-nuclease mapping. The promoter is followed by four open reading frames, designated ugpB, A, E and C, which encode a periplasmic binding protein, two hydrophobic membrane proteins and a protein that is likely to couple energy to the transport system, respectively. The sequences of the proteins contain the characteristics of several other binding protein-dependent transport systems, but they seem to be particularly closely related to the maltose system.
authors.affiliation: Department of Molecular Cell Biology, State University of Utrecht, The Netherlands.
authors.forename: P
authors.initials: P
authors.lastname: Overduin
authors.forename: W
authors.initials: W
authors.lastname: Boos
authors.forename: J
authors.initials: J
authors.lastname: Tommassen
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D001693:Biological Transport, Active; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D005798:Genes, Bacterial; D005994:Glycerophosphates / Q000493:pharmacokinetics; D008320:Maltose / Q000493:pharmacokinetics; D008969:Molecular Sequence Data; D010710:Phosphates / Q000378:metabolism; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
chemical_list: D004269:DNA, Bacterial; D005994:Glycerophosphates; D010710:Phosphates; D008320:Maltose; C029620:alpha-glycerophosphoric acid