On-line fast atom bombardment analysis of dynamic biological systems.
The continuous-flow fast atom bombardment probe interface has been used for the direct analysis of aqueous biochemical reactions. This device was used in two ways: in a constant flow mode and a flow injection mode. Each method has particular advantages which are illustrated with specific applications, including measurement of enzyme kinetics, comparative rates of hydrolysis of multi-substrate/single enzyme reactions, C-terminal peptide sequence analysis, and characterization of multi-enzyme/single substrate systems.
Caprioli
R M
RM
Department of Biochemistry and Molecular Biology, University of Texas Medical School, Houston 77030.
D002851:Chromatography, High Pressure Liquid; D003201:Computers; D013058:Mass Spectrometry / Q000295:instrumentation*; D010447:Peptide Hydrolases; D011492:Protein Hydrolysates / Q000032:analysis
D011492:Protein Hydrolysates; D010447:Peptide Hydrolases