[Purification of restriction endonuclease EcoRII using monoclonal antibodies].
Monoclonal antibodies against EcoRII endonuclease were obtained after immunization of two BALB/c mice with a homogeneous enzyme prepared by conventional methods. IgG from ascitic fluid was purified and coupled to CNBr-activated Sepharose 4B to give a specific column used to isolate EcoRII endonuclease. The isolated EcoRII endonuclease produced a single band during SDS gel electrophoresis.
Kosykh
V G
VG
Mantsygin
Iu A
IuA
Sviatukhina
N V
NV
Vitenene
I V
IV
Bur'ianov
Ia I
IaI
D000911:Antibodies, Monoclonal; D015252:Deoxyribonucleases, Type II Site-Specific / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology*; D007163:Immunosorbent Techniques
D000911:Antibodies, Monoclonal; C046545:CCWGG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific
Ochistka restriltsionnoĭ éndonukleazy EcoRII s ispol'zovaniem monoklonal'nykh antitel.