Inositol 1,4,5-trisphosphate-induced Ca2+ release from permeabilized mastocytoma cells.
The effect of inositol 1,4,5-trisphosphate (IP3) on Ca2+ release in the transformed murine mast cells, mastocytoma P-815 cells permeabilized with digitonin was studied. Ca2+ was sequestered by intracellular organelles in the presence of ATP until the medium free Ca2+ concentration was lowered to a new steady-state level. The subsequent addition of IP3 caused a rapid Ca2+ release, which was followed by a slow re-uptake of Ca2+. Fifty percent of the sequestered Ca2+ was released by 10 microM IP3. Maximal Ca2+ release occurred at 10 microM and half maximal activity was at 1.3 microM. These results indicate that IP3 may function as a messenger of intracellular Ca2+ mobilization in mastocytoma cells.
Muto
Y
Y
Tohmatsu
T
T
Yoshioka
S
S
Nozawa
Y
Y
D000255:Adenosine Triphosphate / Q000502:physiology; D000634:Aminoquinolines; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002451:Cell Compartmentation / Q000187:drug effects; D002460:Cell Line; D002463:Cell Membrane Permeability / Q000187:drug effects; D004072:Digitonin / Q000494:pharmacology; D004721:Endoplasmic Reticulum / Q000378:metabolism; D007295:Inositol Phosphates / Q000494:pharmacology*; D012515:Mast-Cell Sarcoma / Q000378:metabolism*; D051379:Mice; D013403:Sugar Phosphates / Q000494:pharmacology*
D000634:Aminoquinolines; D007295:Inositol Phosphates; D013403:Sugar Phosphates; C035738:Quin2-acetoxymethyl ester; D000255:Adenosine Triphosphate; D004072:Digitonin; D002118:Calcium