[Characteristics of lysine transport in a wild type strain and lysine-producing mutant of Corynebacterium glutamicum].
An active transport system high specific for 1-lysine was found in the cells of the wild strain of Corynebacterium glutamicum, Km being about 10 microM. Accumulation of lysine was higher, if the cells were cultivated on a medium containing glucose. The cells of the homoserine-deficient lysine producer have no alterations in the lysine transport. The lysine transport was also studied in three lysine producing analog resistant mutants (two mutants are resistant to aminoethylcysteine and one to lysine hydroxamate). The key enzyme of the lysine biosynthesis, aspartate kinase, is insensitive to the feedback inhibition by the mixture of lysine and threonine in all the mutant studied; at the same time the cells of these mutants grown on a glucose-containing medium above mentioned alterations are suggested to provide the resistance to the lysine analog.
Lunts
M G
MG
Gusiatiner
M M
MM
Kopteva
A V
AV
Zhdanova
N I
NI
D001692:Biological Transport; D003352:Corynebacterium / Q000235:genetics / Q000378:metabolism*; D003470:Culture Media; D008239:Lysine / Q000096:biosynthesis / Q000378:metabolism*; D009154:Mutation
D003470:Culture Media; D008239:Lysine
Osobennosti transporta lizina v kletki shtamma Corynebacterium glutamicum dikogo tipa i lizinprodutsiruiushchikh mutantov.