title: Change in the synthesis of glycosaminoglycans by fibrotic vitreous induced by erythrocytes.
abstract: Vitreous fibrosis was induced in rabbit eyes by intravitreal injection of erythrocytes. The fibrotic vitreous removed from experimental animals were then incubated with [3H]glucosamine at 37 degrees C for 24 h. The newly synthesized 3H-labeled glycosaminoglycans were isolated by 4 M guanidium hydrochloride extraction followed by pronase digestion. The 3H-labeled glycosaminoglycans were then characterized by gel filtration column chromatography and by specific enzymatic degradation, i.e., hyaluronidase, chondroitinase AC, and/or chondroitinase ABC. The disaccharides derived from chondroitinase ABC degradation were identified by thin-layer chromatography. We previously demonstrated that 91% of the total glycosaminoglycan synthesized by normal vitreous was hyaluronic acid. Our present results indicate that in the fibrotic vitreous, the synthesis of hyaluronic acid was decreased to 26%, whereas the synthesis of chondroitin sulfate increased to 59% of the total newly synthesized glycosaminoglycans. These results suggest that cells present in fibrotic vitreous resemble fibroblasts with respect to their activities in glycosaminoglycans synthesis.
authors.forename: C
authors.initials: C
authors.lastname: Katakami
authors.forename: L A
authors.initials: LA
authors.lastname: Raymond
authors.forename: M J
authors.initials: MJ
authors.lastname: Lipman
authors.forename: A
authors.initials: A
authors.lastname: Appel
authors.forename: W W
authors.initials: WW
authors.lastname: Kao
mesh_terms: D000818:Animals; D002809:Chondroitin Sulfates / Q000096:biosynthesis; D004912:Erythrocytes; D005128:Eye Diseases / Q000378:metabolism; D005260:Female; D006025:Glycosaminoglycans / Q000096:biosynthesis*; D006820:Hyaluronic Acid / Q000096:biosynthesis; D008297:Male; D011817:Rabbits; D014822:Vitreous Body / Q000378:metabolism*
chemical_list: D006025:Glycosaminoglycans; D006820:Hyaluronic Acid; D002809:Chondroitin Sulfates