Differential identification of Candida species and other yeasts by analysis of [35S]methionine-labeled polypeptide profiles.
This paper describes a scheme for differential identification of Candida species and other yeasts based on autoradiographic analysis of protein profiles of [35S]methionine-labeled cellular proteins separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Using ATCC strains as references, protein profile analysis showed that different Candida and other yeast species produced distinctively different patterns. Good agreement in results obtained with this approach and with other conventional systems was observed. Being accurate and reproducible, this approach provides a basis for the development of an alternative method for the identification of yeasts isolated from clinical specimens.
Shen
H D
HD
Department of Medical Research, Veterans General Hospital, Taipei, Taiwan, Republic of China.
Choo
K B
KB
Tsai
W C
WC
Jen
T M
TM
Yeh
J Y
JY
Han
S H
SH
D002175:Candida / Q000145:classification*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D008715:Methionine / Q000378:metabolism*; D008970:Molecular Weight; D010455:Peptides / Q000032:analysis*; D011865:Radioisotope Dilution Technique; D013045:Species Specificity; D013462:Sulfur Radioisotopes; D015003:Yeasts / Q000145:classification*
D010455:Peptides; D013462:Sulfur Radioisotopes; D008715:Methionine