title: LHRH stimulates plasminogen activator and inhibits steroid production by granulosa cells of adult rat Graafian follicles.
abstract: We studied the effects of LHRH and its analogs on plasminogen activator production and progesterone and estradiol secretion by granulosa cells isolated from adult rat Graafian follicles. LHRH and its agonist ([des- -Gly10,D-Trp6,Pro9-NHEt]LHRH) stimulated small but significant increases in plasminogen activator production. This stimulatory action of LHRH was blocked by the addition of the specific antagonist ([D- pGlu1,D-Phe2,D-Trp3,6]LHRH). In contrast, LHRH treatment had no significant effect on basal steroid production by granulosa cells of Graafian follicles cultured for 2-6 days. However, LHRH decreased the FSH-stimulated steroidogenesis. This inhibitory effect of LHRH on steroidogenesis was abolished by concomitant addition of the LHRH antagonist. The results show that LHRH has a stimulatory effect on plasminogen activator production and a suppressive action on steroidogenesis in granulosa cells isolated from Graafian follicles of adult rats.
authors.forename: C
authors.initials: C
authors.lastname: Wang
authors.forename: A
authors.initials: A
authors.lastname: Leung
mesh_terms: D000818:Animals; D004958:Estradiol / Q000096:biosynthesis; D005260:Female; D005342:Fibrinolysis / Q000187:drug effects; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D012739:Gonadal Steroid Hormones / Q000096:biosynthesis*; D007987:Gonadotropin-Releasing Hormone / Q000031:analogs & derivatives / Q000494:pharmacology*; D006107:Granulosa Cells / Q000378:metabolism*; D066298:In Vitro Techniques; D006080:Ovarian Follicle / Q000378:metabolism*; D010960:Plasminogen Activators / Q000096:biosynthesis*; D011359:Proestrus; D011374:Progesterone / Q000096:biosynthesis; D011761:Pyrrolidonecarboxylic Acid / Q000031:analogs & derivatives; D051381:Rats; D011919:Rats, Inbred Strains; D017329:Triptorelin Pamoate / Q000031:analogs & derivatives*
chemical_list: D012739:Gonadal Steroid Hormones; D017329:Triptorelin Pamoate; D007987:Gonadotropin-Releasing Hormone; D011374:Progesterone; D004958:Estradiol; C019176:Tryptal; C034511:LHRH, pGlu(1)-Phe(2)-Trp(3,6)-; D005640:Follicle Stimulating Hormone; D010960:Plasminogen Activators; D011761:Pyrrolidonecarboxylic Acid