title: An analysis of the mutagenicity of 1,2-dibromoethane to Escherichia coli: influence of DNA repair activities and metabolic pathways.
abstract: The mutagenicity of 1,2-dibromoethane (EDB) to Escherichia coli was reduced by the UV light-induced excision repair system but unaffected by the loss of a major apurinic/apyrimidinic site repair function. At high doses, 70-90% of the EDB-induced mutations were independent of SOS-mutagenic processing and approximately 50% were independent of glutathione conjugation. The SOS-independent mutations induced by EDB were unaffected by the enzymes that repair alkylation-induced DNA lesions. EDB-induced base substitutions were dominated by GC to AT and AT to GC transitions. These results suggest that EDB-induced premutagenic lesions have some, but not all, of the characteristics of simple alkyl lesions.
authors.affiliation: Division of Environmental Health, Boston University School of Public Health, Boston University School of Medicine, MA 02118.
authors.forename: P L
authors.initials: PL
authors.lastname: Foster
authors.forename: W G
authors.initials: WG
authors.lastname: Wilkinson
authors.forename: J K
authors.initials: JK
authors.lastname: Miller
authors.forename: A D
authors.initials: AD
authors.lastname: Sullivan
authors.forename: W M
authors.initials: WM
authors.lastname: Barnes
mesh_terms: D001711:Biotransformation; D004260:DNA Repair; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics; D015946:Ethylene Dibromide / Q000633:toxicity*; D006842:Hydrocarbons, Brominated / Q000633:toxicity*; D066298:In Vitro Techniques; D008862:Microsomes, Liver / Q000378:metabolism; D009152:Mutagenicity Tests; D009154:Mutation / Q000187:drug effects*; D013014:SOS Response, Genetics
chemical_list: D006842:Hydrocarbons, Brominated; D015946:Ethylene Dibromide