Expression of the staphylococcal protein A gene in Bacillus subtilis by integration of the intact gene into the B. subtilis chromosome.
Staphylococcal protein A was synthesized at high levels and was secreted efficiently into the culture medium by strains of Bacillus subtilis in which the cloned gene (spa) from Staphylococcus aureus 8325-4 was inserted into the chromosome. The spa gene could not be established in B. subtilis on multicopy plasmids.
Fahnestock
S R
SR
Saunders
C W
CW
Guyer
M S
MS
Löfdahl
S
S
Guss
B
B
Uhlén
M
M
Lindberg
M
M
D001412:Bacillus subtilis / Q000235:genetics* / Q000378:metabolism; D002876:Chromosomes, Bacterial; D003001:Cloning, Molecular; D005796:Genes; D005798:Genes, Bacterial; D010957:Plasmids; D011995:Recombination, Genetic; D013205:Staphylococcal Protein A / Q000096:biosynthesis / Q000235:genetics*; D014169:Transformation, Bacterial
D013205:Staphylococcal Protein A