title: Functionally important conserved amino-acids in interferon-alpha 2 identified with analogues produced from synthetic genes.
abstract: A gene was chemically synthesised and expressed in Escherichia coli to produce [Ala30,32,33]IFN-alpha 2, an analogue of human alpha 2-interferon (IFN-alpha 2) which is devoid of activity on human cells. Eight additional analogues provided single changes in IFN-alpha 2 at each of these three conserved positions. No one residue is essential for activity, but both antiviral and anti-proliferative activity are particularly sensitive to changes in the side-chain of Arg33.
authors.forename: R
authors.initials: R
authors.lastname: Camble
authors.forename: N N
authors.initials: NN
authors.lastname: Petter
authors.forename: P
authors.initials: P
authors.lastname: Trueman
authors.forename: C R
authors.initials: CR
authors.lastname: Newton
authors.forename: F J
authors.initials: FJ
authors.lastname: Carr
authors.forename: R C
authors.initials: RC
authors.lastname: Hockney
authors.forename: V E
authors.initials: VE
authors.lastname: Moore
authors.forename: A R
authors.initials: AR
authors.lastname: Greene
authors.forename: D
authors.initials: D
authors.lastname: Holland
authors.forename: M D
authors.initials: MD
authors.lastname: Edge
mesh_terms: D000596:Amino Acids / Q000032:analysis*; D004274:DNA, Recombinant; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D005796:Genes; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics / Q000502:physiology*; D010957:Plasmids
chemical_list: D000596:Amino Acids; D004274:DNA, Recombinant; D007371:Interferon-gamma