[Morphological studies of the effect of chromium-III-chloride on the islet cell organ in rats under the conditions of high and low fat diets].
It is well known, that the glucose tolerance factor amplifies the peripheral action of insulin. This factor contains trivalent chromium. This investigation deals with the influence of trivalent chromium on morphometric and histological parameters of pancreatic islets of wistar rats. Animals were given chromium-III-chloride for 8 weeks (perorally, daily 15 ppm or 3 ppm) under conditions of high (HFD) and low (LFD) fat diet. Staining of pancreatic slices was performed with Victoria blue and acid Fuchsin. The relative volume density of the endocrine cells was obtained as the ratio of the number of islet cells to the number of exocrine pancreatic cells. The statistical evaluation was performed by using Students t-test. In HFD animals (n = 12) chromium supplementation caused an increase by 41% of volume of islets compared to HFD controls. A lot of small islets were found, which may be newly formed. Moreover, polynesia and hyperplasia of islets were observed. LFD animals (n = 12) do not show any morphometric changes in comparison with LED controls; but macronesia, micronesia and polynesia of islets were observed. In conclusion we suggest, that under special conditions trivalent chromium has growth stimulatory effects on the endocrine pancreas.
Hübner
G
G
Institut für Anatomie des Bereichs Medizin, Ernst-Moritz-Arndt-Universität Greifswald, DDR.
von Dorsche
H H
HH
Zühlke
H
H
D000821:Animal Feed; D000818:Animals; D002712:Chlorides; D002857:Chromium / Q000494:pharmacology*; D017608:Chromium Compounds; D004041:Dietary Fats / Q000008:administration & dosage*; D007515:Islets of Langerhans / Q000187:drug effects* / Q000254:growth & development; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D002712:Chlorides; D017608:Chromium Compounds; D004041:Dietary Fats; D002857:Chromium; C022990:chromic chloride
Morphologische Untersuchungen zur Wirkung von Chrom-III-Chlorid auf das Inselzellorgan bei Ratten unter den Bedingungen einer Hochfett- und einer Niedrigfettdiät.