title: Synthesis and expression of a synthetic gene for the activated human c-Ha-ras protein.
abstract: A 576 base-pair DNA encoding human c-Ha-ras protein (p21) with a valine codon at position 12 has been synthesized by ligation of chemically synthesized oligodeoxyribonucleotides. The duplex was inserted into a plasmid with the E. coli tryptophan promoter and was expressed in E. coli efficiently.
authors.forename: K
authors.initials: K
authors.lastname: Miura
authors.forename: Y
authors.initials: Y
authors.lastname: Inoue
authors.forename: H
authors.initials: H
authors.lastname: Nakamori
authors.forename: S
authors.initials: S
authors.lastname: Iwai
authors.forename: E
authors.initials: E
authors.lastname: Ohtsuka
authors.forename: M
authors.initials: M
authors.lastname: Ikehara
authors.forename: S
authors.initials: S
authors.lastname: Noguchi
authors.forename: S
authors.initials: S
authors.lastname: Nishimura
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D013006:Growth Hormone / Q000235:genetics; D006801:Humans; D010957:Plasmids; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis*; D011519:Proto-Oncogenes
chemical_list: D011518:Proto-Oncogene Proteins; D013006:Growth Hormone