The stimulating effect of a cytosol extract from regenerating liver on isolated hepatocytes and the positive role of insulin.
Several kinds of damage induce a regenerative response in the liver. Partial hepatectomy is a well known method, suitable for studying liver regeneration and factor/s involved in it. The presence of the so called Hepatic Stimulator Substance has been demonstrated in the cytosol obtained from regenerating hepatocytes, and it has been partially characterized, from a molecular point of view. Mainly, it stimulates liver regeneration and Tritiated Thymidine incorporation in cultured hepatocytes as demonstrated in this paper (Control Hepatocytes: 8447 +/- 660.4 cpm, (mean +/- sd), Test Hepatocytes: 16974 +/- 2720.9 cpm; Index of Stimulation: 2.01). Furthermore, the stimulating activity of HSS is increased by the presence, in the culture media, of insulin which has been proved to have a positive role in liver regeneration (Control Hepatocytes: 8889 +/- 367.3 cpm; Test Hepatocytes: 21588.5 +/- 1232.4 cpm; Index of Stimulation: 2.42, p less than 0.05). These data seem to suggest that liver is probably the site of production/activity and action of factor/s that stimulate/s liver regeneration; but other substances exist, such as insulin, which play a permissive role.
Gorini
P
P
Department of Surgery, University of Toronto, Canada.
Koshikawa
H
H
Falk
R E
RE
D000818:Animals; D003600:Cytosol; D005260:Female; D006498:Hepatectomy; D007328:Insulin / Q000494:pharmacology; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D008115:Liver Regeneration; D051381:Rats
D007328:Insulin