The role of monocyte cytotoxic factor in monocyte-mediated lysis of tumour cells.
The role of a monocyte cytotoxic factor (CF) in monocyte-mediated lysis of leukaemia cells (K562) has been investigated using a polyclonal rabbit antiserum raised against purified CF. The CF antiserum inhibited K562 cell lysis mediated by interferon gamma (IFN-gamma)-activated monocytes. CF antiserum also inhibited monocyte-mediated lysis of antibody-coated K562 cells (AbK562). Preimmune serum at the same concentration as CF antiserum did not affect monocyte-mediated lysis, and the CF antiserum did not inhibit binding between effector and target cells, indicating that inhibition of monocyte-mediated lysis by CF antiserum was not merely a result of toxic components present in the rabbit serum, or a result of a decrease in monocyte-target cell binding. Taken together, the data suggest that CF is involved in monocyte-mediated lysis of uncoated as well as antibody-coated K562 cells.
Espevik
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Kildahl-Andersen
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Nissen-Meyer
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D000328:Adult; D000920:Antibody-Dependent Cell Cytotoxicity; D002454:Cell Differentiation; D002478:Cells, Cultured; D003603:Cytotoxins / Q000276:immunology*; D004306:Dose-Response Relationship, Immunologic; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D007371:Interferon-gamma / Q000494:pharmacology; D007942:Leukemia, Experimental / Q000276:immunology*; D009000:Monocytes / Q000276:immunology*
D003603:Cytotoxins; D007106:Immune Sera; D007371:Interferon-gamma