title: Lack of lamins A and C in mammalian hemopoietic cell lines devoid of intermediate filament proteins.
abstract: Using immunofluorescence microscopy and immunoblot analysis, we have examined the composition of the nuclear lamina in several murine and human cell lines. Whereas it was shown that intermediate filament-positive Ehrlich ascites tumor and HeLa-S3 cells contain the three major mammalian lamin subspecies, only lamin B could be detected in several myeloid- and lymphoid-derived cell lines representative of distinct stages in hemopoietic differentiation but all devoid of cytoplasmic intermediate filament proteins. These included the murine plasmacytoma cell types MPC-11 and MOPC-31C, murine myeloma cells X63-Ag8.6.5.3 and human promyelocytic leukemia cells HL-60. Our results provide the first evidence that mammalian somatic cells capable of normal proliferation may lack both cytoplasmic intermediate filament proteins and a normal complement of nuclear lamins.
authors.affiliation: Max-Planck-Institut für Zellbiologie, Ladenburg bei Heidelberg/Bundesrepublik Deutschland.
authors.forename: M
authors.initials: M
authors.lastname: Paulin-Levasseur
authors.forename: A
authors.initials: A
authors.lastname: Scherbarth
authors.forename: U
authors.initials: U
authors.lastname: Traub
authors.forename: P
authors.initials: P
authors.lastname: Traub
mesh_terms: D000818:Animals; D002460:Cell Line; D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation; D006801:Humans; D015151:Immunoblotting; D007381:Intermediate Filament Proteins / Q000502:physiology / Q000648:ultrastructure*; D034921:Lamin Type B; D034882:Lamins; D051379:Mice; D008854:Microscopy, Electron; D009687:Nuclear Proteins / Q000032:analysis* / Q000502:physiology; D014407:Tumor Cells, Cultured / Q000032:analysis / Q000648:ultrastructure
chemical_list: D007381:Intermediate Filament Proteins; D034921:Lamin Type B; D034882:Lamins; D009687:Nuclear Proteins