A new method to prepare sections from amphibian embryos for immunohistology.
The method involves impregnation of fixed embryos with a solution of plexiglass. The solvent is evaporated slowly. Blocks of plexiglass containing the specimen are thus formed. Sections (1-2 microns) are stretched and attached by an unconvential method. Immunohistological techniques allow antigen localization in a combination of high resolution, excellent quality of the sections and low background.
Hausen
P
P
Max-Planck-Institut für Entwicklungsbiologie, Abteilung Zellbiologie, Tübingen, Bundesrepublik Deutschland.
D000818:Animals; D004625:Embryo, Nonmammalian / Q000166:cytology*; D005260:Female; D006652:Histological Techniques; D007202:Indicators and Reagents; D009865:Oocytes / Q000166:cytology*; D014982:Xenopus laevis
D007202:Indicators and Reagents