Studies on the hexose region of the lipopolysaccharide from a low virulence strain of Salmonella typhimurium.
The structure of the hexose region of the lipopolysaccharide from M206 strain, a mutant of Salmonella typhimurium having reduced virulence, was partially determined. Immunological tests indicated cross-reactions of anti-(M206) antiserum with wild-type C5 and Ra mutant strains. Data obtained on chemical composition, periodate oxidation, acetolysis, methylation and analysis by gas chromatography/mass spectrometry show that M206 type lipopolysaccharide contains the common core polysaccharide of Salmonella which was substituted in position 4 of the subterminal glucose unit by a disaccharide: D-glucosyl 1----3 D-galactose. This substitution is probably related to the slight virulence of M206 strain.
dalla Venezia
N
N
Laboratoire de Biochimie Microbienne, UA CNRS 1176, Université Lyon I, Villeurbanne, France.
Bruneteau
M
M
Binder
P
P
Creach
O
O
Fontanges
R
R
Michel
G
G
D000085:Acetates; D006601:Hexoses / Q000032:analysis*; D007120:Immunochemistry; D008070:Lipopolysaccharides / Q000032:analysis* / Q000276:immunology; D008745:Methylation; D010084:Oxidation-Reduction; D012486:Salmonella typhimurium / Q000032:analysis* / Q000276:immunology / Q000472:pathogenicity; D014774:Virulence / Q000235:genetics
D000085:Acetates; D006601:Hexoses; D008070:Lipopolysaccharides