Isolation and characterization of a mouse interleukin cDNA clone that expresses B-cell stimulatory factor 1 activities and T-cell- and mast-cell-stimulating activities.
A cDNA sequence coding for a unique mouse interleukin that expresses B-cell-, T-cell, and mast-cell-stimulating activities has been isolated from a mouse helper T-cell cDNA library. The library, constructed in the pcD expression vector, was screened by transfecting COS monkey cells with DNA pools to express the products encoded by full-length cDNA inserts. By assaying the transfected cell supernatants, we identified clones encoding a factor that stimulates T-cell and mast cell lines. This factor also induces Ia expression on resting B cells and enhances IgG1 and IgE production by B cells, two properties of B-cell-stimulatory factor 1. The DNA sequence codes for a polypeptide of 140 amino acid residues including a putative signal peptide. These results demonstrate that a single cDNA clone distinct from interleukin 2 and interleukin 3 encodes a polypeptide with multiple biological activities.
Lee
F
F
Yokota
T
T
Otsuka
T
T
Meyerson
P
P
Villaret
D
D
Coffman
R
R
Mosmann
T
T
Rennick
D
D
Roehm
N
N
Smith
C
C
D000595:Amino Acid Sequence; D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology*; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D006133:Growth Substances / Q000235:genetics* / Q000502:physiology; D007376:Interleukin-2 / Q000235:genetics* / Q000502:physiology; D007377:Interleukin-3; D015847:Interleukin-4; D008222:Lymphokines / Q000235:genetics* / Q000502:physiology; D008407:Mast Cells / Q000166:cytology*; D051379:Mice; D013601:T-Lymphocytes / Q000166:cytology / Q000502:physiology*
D006133:Growth Substances; D007376:Interleukin-2; D007377:Interleukin-3; D008222:Lymphokines; D015847:Interleukin-4; D004247:DNA