Cloning in Yersinia enterocolitica of K99 antigen gene from Escherichia coli.
A recombinant plasmid pFS239 containing the gene coding for K99 antigen of Escherichia coli and wide-host-range plasmid pKT230 has been cloned in E. coli C600. pFS239 has been transferred to Yersinia enterocolitica strains D29, L15 and L15 (pYV15) through triparental mating. In Y. enterocolitica transconjugants the expression of VW antigens and calcium dependence which represent the properties associated with the virulence plasmid of Y. enterocolitica remains unchanged.
Sun
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WL
Biotechnology Research Center, Chinese Academy of Agricultural Sciences, Beijing.
Fan
Y L
YL
D000954:Antigens, Surface / Q000235:genetics; D001427:Bacterial Toxins; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics*; D010957:Plasmids; D014162:Transfection; D015008:Yersinia enterocolitica / Q000235:genetics*
D000954:Antigens, Surface; D001427:Bacterial Toxins; C031429:K99 antigen