Protein engineering in studies on metabolism of toxic substances.
Expression of cytochrome P-450s, drug metabolizing enzymes, from their coding sequences in heterologous cells were developed and the enzymatically active P-450s were synthesized. cDNAs of various chimera or mutant P-450s were constructed and expressed. Thus, regions or residues important for the function of cytochrome P-450 were analyzed.
Imai
Y
Y
Institute for Protein Research, Osaka University, Japan.
D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D002678:Chimera; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis / Q000235:genetics* / Q000502:physiology; D004247:DNA / Q000235:genetics; D064420:Drug-Related Side Effects and Adverse Reactions; D005815:Genetic Code; D005818:Genetic Engineering / Q000379:methods*; D051379:Mice; D004364:Pharmaceutical Preparations / Q000378:metabolism*; D011817:Rabbits; D051381:Rats; D013329:Structure-Activity Relationship
D004364:Pharmaceutical Preparations; D004247:DNA; D003577:Cytochrome P-450 Enzyme System