title: The lactose carrier of Klebsiella pneumoniae M5a1; the physiology of transport and the nucleotide sequence of the lacY gene.
abstract: A comparison has been made between the physiology and amino acid sequence of the lactose carriers of Klebsiella pneumoniae M5a1 and Escherichia coli K-12. The membrane transport of lactose was much weaker in Klebsiella than in E. coli. On the other hand o-nitrophenylgalactoside uptake by Klebsiella was distinctly greater than with E. coli. In spite of the differences in sugar transport between the two organisms, the amino acid sequences of the respective lactose carriers were remarkably similar (60% of the amino acids are identical).
authors.affiliation: Department of Cellular and Molecular Physiology, Harvard Medical School Boston, MA 02115.
authors.forename: I
authors.initials: I
authors.lastname: McMorrow
authors.forename: D T
authors.initials: DT
authors.lastname: Chin
authors.forename: K
authors.initials: K
authors.lastname: Fiebig
authors.forename: J L
authors.initials: JL
authors.lastname: Pierce
authors.forename: D M
authors.initials: DM
authors.lastname: Wilson
authors.forename: E C
authors.initials: EC
authors.lastname: Reeve
authors.forename: T H
authors.initials: TH
authors.lastname: Wilson
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D001692:Biological Transport; D004252:DNA Mutational Analysis; D029968:Escherichia coli Proteins; D005697:Galactosides / Q000378:metabolism; D005798:Genes, Bacterial; D007711:Klebsiella pneumoniae / Q000502:physiology*; D007785:Lactose / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000235:genetics / Q000502:physiology*; D008969:Molecular Sequence Data; D009004:Monosaccharide Transport Proteins; D015183:Restriction Mapping; D027981:Symporters
chemical_list: D029968:Escherichia coli Proteins; D005697:Galactosides; C050221:LacY protein, E coli; D026901:Membrane Transport Proteins; D009004:Monosaccharide Transport Proteins; D027981:Symporters; C021499:lactose permease; D007785:Lactose