Cloning of the HhaI and HinPI restriction-modification systems.
The genes for the HhaI (Roberts et al., 1976) and HinPI (Roberts, 1987) restriction-modification (R-M) systems have been cloned in pBR322. The HhaI system was isolated on a 9-kb PstI fragment, and the HinPI system was isolated on two PstI fragments of 1.5 and 4.6 kb in length. The clones were isolated by selecting for recombinant molecules that had protectively modified themselves. The HhaI and HinPI R-M systems recognize the same sequence, GCGC, but hybridization between the DNA fragments encoding them does not take place.
Barsomian
J M
JM
New England Biolabs, Inc., Beverly, MA 01915.
Card
C O
CO
Wilson
G G
GG
D001426:Bacterial Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D015257:DNA-Cytosine Methylases / Q000235:genetics*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D006190:Haemophilus / Q000201:enzymology / Q000235:genetics*; D006193:Haemophilus influenzae / Q000201:enzymology / Q000235:genetics*; D011994:Recombinant Proteins / Q000235:genetics
D001426:Bacterial Proteins; D011994:Recombinant Proteins; C037664:DNA modification methylase HhaI; D015257:DNA-Cytosine Methylases; D015252:Deoxyribonucleases, Type II Site-Specific; C037130:GCGC-specific type II deoxyribonucleases