The synthesis, cloning and expression of a repeating segment of the circumsporozoite surface protein of Plasmodium falciparum.
Repeats of the tetrapeptide (NANP) of the circumsporozoite protein of P. falciparum have been found to be immunogenic and possibly may act as vaccines against malaria infection. The DNA duplex encoding for eight of the (NANP) repeating unit and two of the (NVDP) unit have been synthesized, cloned and expressed in E. coli as a fused protein. The recombinant protein was shown to be immunogenic and to have the antigenic activity of the circumsporozoite.
Armah
G E
GE
Faculty of Pharmaceutical Sciences, Osaka University, Japan.
Nishikawa
S
S
Kondo
C
C
Tomita
K
K
D000595:Amino Acid Sequence; D000818:Animals; D000954:Antigens, Surface / Q000235:genetics*; D003001:Cloning, Molecular / Q000379:methods; D005796:Genes; D008969:Molecular Sequence Data; D010963:Plasmodium falciparum / Q000235:genetics*; D015800:Protozoan Proteins; D012091:Repetitive Sequences, Nucleic Acid; D014158:Transcription, Genetic
D000954:Antigens, Surface; D015800:Protozoan Proteins; C041595:circumsporozoite protein, Protozoan