[The choice of rational methods for determining antibodies to Yersinia enterocolitica. The agglutination reaction].
Six tests suitable for the detection of antibodies to Y. enterocolitica, serovars 03 and 09, were studied. The results of the study of hyperimmune sera revealed that the agglutination test and the indirect hemagglutination test were the most promising methods and deserved efforts for their further development. Bacterial diagnostic agents were prepared from live, heat-killed and formalin-killed Yersinia cultures and tested. The titration of homologous and heterologous sera revealed no essential differences in the preparations; in the serological examination of healthy persons (102), patients with Yersinia enteral infection (150) and with other acute enteral infections (92), the results obtained with the use of the above-mentioned preparations carried the highest information content. OH diagnostic agents proved to have the highest specificity; of these, the diagnosticum obtained by treatment 0.3-0.4% formalin vas found to be the most stable and technologically effective preparation. The minimal diagnostic titer of the agglutination test for the presence of Yersinia enteral infection was established (1:60 = 1:320). This titer was determined in 73% of the patients with bacteriologically confirmed Yersinia enteral infection and only in 1% of healthy persons and patients with other acute enteral infections.
Koroliuk
A M
AM
Golovacheva
S N
SN
Dulatova
M V
MV
D000208:Acute Disease; D000372:Agglutination Tests; D000907:Antibodies, Bacterial / Q000032:analysis*; D003937:Diagnosis, Differential; D005069:Evaluation Studies as Topic; D005455:Fluorescent Antibody Technique; D006386:Hemagglutination Tests; D006462:Hemolytic Plaque Technique; D006801:Humans; D007410:Intestinal Diseases / Q000175:diagnosis; D011233:Precipitin Tests; D015009:Yersinia Infections / Q000175:diagnosis; D015008:Yersinia enterocolitica / Q000276:immunology*
D000907:Antibodies, Bacterial
Vybor ratsional'nykh metodov opredeleniia antitel k Yersinia enterocolitica. Reaktsiia aggliutinatsii.