Localization of the KRAS2 oncogene in the domestic rabbit (Oryctolagus cuniculus).
The KRAS2 gene was localized in the rabbit by chromosomal in situ hybridization, using a 3H-labeled human cDNA probe. There were 201 silver grains on 144 metaphase spreads; 12.9% of the grains resided on chromosome 16, and 54% of these grains were located close to the centromere at 16p11----q11. Statistical analysis indicated that labeling at this region represents a significant deviation from a random distribution and thus provided evidence for the assignment of KRAS2 to 16p11----q11. In addition to the predominant labeling site on 16, there was a positive signal at the telomere of 9q, possibly representing a sequence of another member of the ras family. Our assignment of KRAS2 to rabbit chromosome 16 strengthens the argument that a fragment on this chromosome is homologous to one on human chromosome 12, which bears the KRAS2 locus.
Martin-DeLeon
P A
PA
School of Life and Health Sciences, University of Delaware, Newark 19716.
Picciano
S R
SR
D000818:Animals; D002478:Cells, Cultured; D002874:Chromosome Mapping; D002875:Chromosomes / Q000032:analysis / Q000648:ultrastructure; D004247:DNA / Q000032:analysis; D015342:DNA Probes; D005347:Fibroblasts / Q000166:cytology; D011905:Genes, ras; D008677:Metaphase; D009693:Nucleic Acid Hybridization; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D016283:Proto-Oncogene Proteins p21(ras); D011817:Rabbits / Q000235:genetics*
D015342:DNA Probes; D011518:Proto-Oncogene Proteins; D004247:DNA; D016283:Proto-Oncogene Proteins p21(ras)