The measurement of protein degradation in response to oxidative stress.
This paper briefly outlines several different systems in which radical/oxidant effects on protein degradation can be studied. Erythrocytes, reticulocytes, skeletal muscles, bacteria, mitochondria, cell lysates, cell-free extracts, and isolated proteases are all useful systems, each of which has different advantages and limitations. It is hoped that dissemination of these techniques and approaches will stimulate further study of protein degradation, both as a result and as an index of oxidative stress.
Pacifici
R E
RE
Institute for Toxicology, University of Southern California, Los Angeles 90033.
Lin
S W
SW
Davies
K J
KJ
D000409:Alanine / Q000032:analysis; D000818:Animals; D001419:Bacteria / Q000378:metabolism; D002474:Cell-Free System; D004912:Erythrocytes / Q000378:metabolism; D005609:Free Radicals; D006801:Humans; D006868:Hydrolysis; D008722:Methods; D009132:Muscles / Q000378:metabolism; D010084:Oxidation-Reduction; D010447:Peptide Hydrolases / Q000378:metabolism; D011506:Proteins; D012156:Reticulocytes / Q000378:metabolism
D005609:Free Radicals; D011506:Proteins; D010447:Peptide Hydrolases; D000409:Alanine