Location of DNA-binding segment of a positive regulator, OmpR, involved in activation of the ompF and ompC genes of Escherichia coli.
OmpR protein is a positive regulator involved in activation of the ompF and ompC genes which encode the major outer membrane proteins OmpF and OmpC, respectively. OmpR protein is considered to have a two-domain structure. In the present study we isolated a C-terminal fragment of the OmpR molecule, which bound to a specific promoter sequence of the ompF gene. We conclude that the C-terminal portion of the OmpR protein contains a DNA-binding site.
Tate
S
S
Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
Kato
M
M
Nishimura
Y
Y
Arata
Y
Y
Mizuno
T
T
D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D001483:Base Sequence; D001665:Binding Sites; D004269:DNA, Bacterial / Q000378:metabolism*; D003850:Deoxyribonuclease I; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010446:Peptide Fragments / Q000302:isolation & purification / Q000378:metabolism*; D011401:Promoter Regions, Genetic
D001425:Bacterial Outer Membrane Proteins; D004269:DNA, Bacterial; D010446:Peptide Fragments; D003850:Deoxyribonuclease I