title: Point mutations in the 23 S rRNA genes of four lincomycin resistant Nicotiana plumbaginifolia mutants could provide new selectable markers for chloroplast transformation.
abstract: Experiments designed to establish stable chloroplast transformation require selectable marker genes encoded by the chloroplast genome. The antibiotic lincomycin is a specific inhibitor of chloroplast ribosomal activity and is known to bind to the large ribosomal subunit. We have investigated a defined region of the chloroplast 23 S rRNA genes from four lincomycin resistant Nicotiana plumbaginifolia mutants and from wild-type N. plumbaginifolia. The mutants LR415, LR421 and LR446 have A to G transitions at positions equivalent to the nucleotides 2058 and 2059 in the Escherichia coli 23 S rRNA. The mutant, LR400, possesses a G to A transition at a position corresponding to nucleotide 2032 of the E. coli 23 S rRNA.
authors.affiliation: Max Planck Institut für Züchtungsforschung, Abteilung Genetische Grundlagen der Pflanzenzüchtung, Köln, Federal Republic of Germany.
authors.forename: A
authors.initials: A
authors.lastname: Cseplö
authors.forename: T
authors.initials: T
authors.lastname: Etzold
authors.forename: J
authors.initials: J
authors.lastname: Schell
authors.forename: P H
authors.initials: PH
authors.lastname: Schreier
mesh_terms: D001483:Base Sequence; D002736:Chloroplasts / Q000502:physiology*; D004351:Drug Resistance / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005796:Genes; D008034:Lincomycin / Q000494:pharmacology*; D008969:Molecular Sequence Data; D009154:Mutation; D009690:Nucleic Acid Conformation; D010944:Plants / Q000187:drug effects / Q000235:genetics*; D010947:Plants, Toxic; D012335:RNA, Ribosomal / Q000235:genetics*; D012338:RNA, Ribosomal, 23S / Q000235:genetics*; D014026:Nicotiana / Q000187:drug effects / Q000235:genetics
chemical_list: D012335:RNA, Ribosomal; D012338:RNA, Ribosomal, 23S; D008034:Lincomycin