Thermal stabilization of putative karyoskeletal protein-enriched fractions from Saccharomyces cerevisiae.
Elevated growth temperature (heat shock) promoted the structural stability of karyoskeletal protein-enriched fractions isolated from Saccharomyces cerevisiae. Similar stabilization could be induced by brief incubation of nuclei at 37 degrees C in vitro. These results are similar to those reported for higher eucaryotes and have practical implications for investigation of the karyoskeleton in S. cerevisiae.
Berrios
S
S
Department of Pharmacological Sciences, State University of New York, Stony Brook 11794-8651.
Fisher
P A
PA
D015153:Blotting, Western; D002467:Cell Nucleus / Q000648:ultrastructure*; D005656:Fungal Proteins / Q000032:analysis*; D006358:Hot Temperature; D008970:Molecular Weight; D009687:Nuclear Proteins / Q000502:physiology*; D012441:Saccharomyces cerevisiae / Q000502:physiology* / Q000648:ultrastructure
D005656:Fungal Proteins; D009687:Nuclear Proteins