Dose relationships between different effects of aphidicolin in JU56 cells.
Some effects of aphidicolin have been investigated in relationship to dose, in a permanent cell line, JU56. Inhibition of semi-conservative DNA synthesis occurred at concentrations greater than 3 X 10(-7) M. In this respect the cells were about as sensitive as L1210 and HeLa cells, and more than 10-fold more sensitive than PHA-stimulated human peripheral blood leucocytes. Delay of progress of cells through G2 occurred at concentrations which inhibited synthesis to about 2% of control levels. Chromatid aberrations appeared in cells at concentrations which decreased synthesis to 5%. Synergism with X-rays in the production of chromatid aberrations occurred at doses which reduced semi-conservative synthesis to 40% of control levels. Isochromatid aberrations appeared in cells continuously exposed to aphidicolin in G2 at concentrations which reduced synthesis to 5% of control units.
Moore
R C
RC
Randell
C
C
Bender
M A
MA
D000818:Animals; D016590:Aphidicolin; D002460:Cell Line; D002869:Chromosome Aberrations / Q000187:drug effects* / Q000528:radiation effects; D004260:DNA Repair / Q000187:drug effects* / Q000528:radiation effects; D004224:Diterpenes / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007621:Karyotyping; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism; D007614:Macropodidae; D013997:Time Factors
D004224:Diterpenes; D016590:Aphidicolin