Expression of tissue-specific genes in transgenic mice.
The ability to introduce cloned genes into mouse germ line has been used for analyzing cis-acting DNA sequences involved in tissue-specific and developmental regulation of the introduced gene. Using this system we have attempted to produce a transgenic mouse model for human dominantly inherited disease, familial amyloidotic polyneuropathy. Recently the mutant transthyretin gene which is considered to be responsible for this disease has been cloned and well characterized at molecular level. We have produced transgenic mice by microinjecting human mutant gene. Amyloid deposition was observed in the mucosa of alimentary tract and renal glomeruli, suggesting that this approach is successful in establishing the mouse model for human genetic disease. In addition, these experiments suggest that the expression of the mutant gene is regulated normally during developmental process and that the cause of adult onset is not due to the dysregulation of this gene expression.
Yamamura
K
K
Institute for Medical Genetics, Kumamoto University Medical School, Japan.
Wakasugi
S
S
Iwanaga
T
T
Inomoto
T
T
Maeda
S
S
Shimada
K
K
D000686:Amyloidosis / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D004195:Disease Models, Animal; D005786:Gene Expression Regulation; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic / Q000378:metabolism*; D011228:Prealbumin / Q000235:genetics / Q000378:metabolism
D011228:Prealbumin