title: Computer-aided detection and alignment of weakly homologous amino acid sequences of RNA replicase beta (MS2 phage) and DNA polymerases (T7 phage and E. coli).
abstract: Alignment of the amino acid (aa) sequences of T7 phage DNA polymerase (DPase), E. coli DNA polymerase I (Pol I) and MS2 phage RNA replicase beta subunit (MS2 Repl) were established by computer-aided methods. The results showed that the entire length (aa's 16-704) of T7 DPase is homologous to Pol I aa's 207-928(C-term) with 21.5% aa identity, and that domains I (aa's-1-311) and II (312-451(C-term] were found to be homologous to each other and to N-terminal region of T7 DPase (aa's 1-250). Thus these enzymes and domains are homologous to one another and must have evolved from a co-ancestral enzyme.
authors.affiliation: Department of Biology, Faculty of Science, Niigata University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Ohnishi
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003090:Coliphages / Q000201:enzymology / Q000235:genetics*; D004259:DNA-Directed DNA Polymerase / Q000235:genetics*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005796:Genes; D005798:Genes, Bacterial; D005814:Genes, Viral; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D012316:RNA Nucleotidyltransferases / Q000235:genetics*; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D012984:Software; D013604:T-Phages / Q000201:enzymology / Q000235:genetics*
chemical_list: D046911:Macromolecular Substances; D012316:RNA Nucleotidyltransferases; D012324:RNA-Dependent RNA Polymerase; D004259:DNA-Directed DNA Polymerase