title: Differing expression of insulin-like growth factor I in the developing and in the adult rat cerebellum.
abstract: Insulin-like growth factor I (IGF-I; somatomedin C) is a trophic peptide of importance for the development of several tissues and organs. In the present study we have mapped the cellular distribution and dynamic changes of IGF-I immunoreactivity in the rat cerebellum from its postnatal development to maturity. In vitro hybridization of IGF-I mRNA was used to demonstrate that the IGF-I immunoreactive material was synthesized in the cerebellum during a limited time period of cerebellar differentiation. IGF-I immunoreactivity was absent in primordial nerve cells but was present in neuroglial cells during the first two days after birth and then rapidly increased in intensity in the latter during the next few days. Proliferative nerve cells in the external granular layer did not express IGF-I immunoreactivity, while migrating and differentiating nerve cells as well as neuroglial cells showed intense labelling. Starting about 2 weeks postnatally, the IGF-I immunoreactivity declined, first in the neuroglial cells and eventually in the nerve cells. No IGF-I immunoreactivity could be demonstrated in the normal adult cerebellum. Colchicine pretreatment did, however, enable demonstration of IGF-I immunoreactivity in adult cerebellar nerve cells but not in neuroglial cells. In vitro hybridization revealed IGF-I mRNA in the developing cerebellum but only at very low levels in the adult cerebellum. It is concluded that IGF-I is likely to be a factor of importance for the development and maturation of nerve cells and neuroglial cells in the brain.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Institute of Neurobiology, University of Göteborg, Sweden.
authors.forename: I K
authors.initials: IK
authors.lastname: Andersson
authors.forename: D
authors.initials: D
authors.lastname: Edwall
authors.forename: G
authors.initials: G
authors.lastname: Norstedt
authors.forename: B
authors.initials: B
authors.lastname: Rozell
authors.forename: A
authors.initials: A
authors.lastname: Skottner
authors.forename: H A
authors.initials: HA
authors.lastname: Hansson
mesh_terms: D000367:Age Factors; D000818:Animals; D002531:Cerebellum / Q000254:growth & development / Q000378:metabolism*; D003078:Colchicine / Q000494:pharmacology; D005455:Fluorescent Antibody Technique; D007334:Insulin-Like Growth Factor I / Q000235:genetics / Q000378:metabolism*; D009457:Neuroglia / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger; D051381:Rats; D011919:Rats, Inbred Strains; D013002:Somatomedins / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D013002:Somatomedins; D007334:Insulin-Like Growth Factor I; D003078:Colchicine