High-frequency transformation of Rhizobium meliloti.
Transformation of R factor RP4 and its derivative pRK290 from Escherichia coli to Rhizobium meliloti is reported. The efficiency of transformation was in the range of 10(-5) per viable cell. In addition, chromosomal DNA prepared from one R. meliloti strain resistant to streptomycin was transferred to the isoleucine-valine-requiring mutant susceptible to streptomycin.
Courtois
J
J
Laboratoire de Microbiologie, U.F.R. de Biologie, Université des Sciences et Techniques de Lille Flandres-Artois, Villeneuve d'Ascq, France.
Courtois
B
B
Guillaume
J
J
D002876:Chromosomes, Bacterial; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D011815:R Factors; D012231:Rhizobium / Q000235:genetics*; D014169:Transformation, Bacterial
D004269:DNA, Bacterial