Early kinetics of human macrophage apolipoprotein E synthesis and incorporation of carbohydrate precursors.
Apolipoprotein E was detected in human macrophage lysates after 4-120-min pulses with [35S]methionine. Significant levels of apolipoprotein E were not found in the media until 120 min. Incorporation of N-[3H]acetylmannosamine, a sialic acid precursor, into apolipoprotein E was measurable after 120 min, whereas no [3H]mannose incorporation could be observed. These methods will be useful for examining the regulation of apolipoprotein E secretion and carbohydrate processing.
Mazzone
T
T
Papagiannes
E
E
Magner
J
J
D000118:Acetylglucosaminidase / Q000378:metabolism; D001057:Apolipoproteins E / Q000096:biosynthesis*; D050260:Carbohydrate Metabolism; D006595:Hexosamines / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D008264:Macrophages / Q000378:metabolism*; D008358:Mannose / Q000378:metabolism; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase; D008715:Methionine / Q000378:metabolism; D013997:Time Factors
D001057:Apolipoproteins E; D006595:Hexosamines; D008715:Methionine; D000118:Acetylglucosaminidase; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase; D008358:Mannose; C002022:N-acetylmannosamine