Transcription of protooncogenes during stimulation of normal human B lymphocytes.
Protooncogenes play important roles in the regulation of growth and differentiation of normal cells. In this study we have examined the cell cycle-dependent regulation of transcription of various protooncogenes after stimulation of human peripheral blood B lymphocytes. The transcriptional rate of various genes was determined by means of a nuclear run-on assay. We found that several protooncogenes were transcriptionally activated after stimulation (myc, p53, K-ras, H-ras, sis and ets), but with different kinetics of induction. In contrast, some oncogenes, especially those encoding membrane-associated or cytoplasmatic proteins like abl, rel or mil/raf, were transcribed at a relatively constant rate during the cell cycle.
Smeland
E B
EB
Department of Pathology, Norwegian Radium Hospital, Oslo, Norway.
Blomhoff
H K
HK
Ohlsson
R
R
De Lange Davies
C
C
Funderud
S
S
Boye
E
E
D001402:B-Lymphocytes / Q000502:physiology*; D002453:Cell Cycle; D005434:Flow Cytometry; D005786:Gene Expression Regulation; D006801:Humans; D066298:In Vitro Techniques; D008213:Lymphocyte Activation; D010982:Platelet-Derived Growth Factor / Q000235:genetics; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D016271:Proto-Oncogene Proteins c-myc; D020574:Proto-Oncogene Proteins c-sis; D011519:Proto-Oncogenes; D014158:Transcription, Genetic
D010982:Platelet-Derived Growth Factor; D011518:Proto-Oncogene Proteins; D016271:Proto-Oncogene Proteins c-myc; D020574:Proto-Oncogene Proteins c-sis