High c-myc amplification level contributes to the tumorigenic phenotype of the human breast carcinoma cell line SW 613-S.
The c-myc gene is amplified in the SW 613-S cell line which was established from a human breast carcinoma. This line is heterogeneous: it contains cells with a high level of amplification and carrying the extra copies of the c-myc gene in double minute chromosomes (DMs) and cells with few c-myc genes integrated into chromosomes. Clones with different levels of amplification and different cytological localization of the c-myc copies were isolated from the SW 613-S cell population. Those with a high level of amplification and expression of the c-myc gene were highly tumorigenic in nude mice whereas those with a low level were not. Introduction of c-myc gene copies by transfection into the cells of several non-tumorigenic clones restored the tumorigenic phenotype. Our results indicate that a high level of amplification of the c-myc gene is a requirement for the tumorigenicity of SW 613-S cells in animals.
Lavialle
C
C
ER 278 CNRS, Institut de Recherches Scientifiques sur le Cancer, Villejuif, France.
Modjtahedi
N
N
Cassingena
R
R
Brison
O
O
D000818:Animals; D001943:Breast Neoplasms / Q000235:genetics / Q000473:pathology*; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000473:pathology; D005260:Female; D005784:Gene Amplification; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009374:Neoplasms, Experimental / Q000209:etiology; D010641:Phenotype; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D016271:Proto-Oncogene Proteins c-myc; D011519:Proto-Oncogenes; D014162:Transfection; D014407:Tumor Cells, Cultured / Q000473:pathology* / Q000637:transplantation
D011518:Proto-Oncogene Proteins; D016271:Proto-Oncogene Proteins c-myc