Cosmids carrying Aspergillus terreus DNA can integrate into Saccharomyces cerevisiae chromosome XII via recombination between yeast and foreign DNAs.
A genome clonotheque consisting of 25- to 40-kb Sau3AI fragments of Aspergillus terreus DNA was constructed in the episomal cosmid vector pES33 containing the yeast ARG4 gene. From the 475 transformants of cir0 yeast strain ESH-0, 23 stable Arg+ transformants were independently selected. Genetic and Southern analysis of these stable transformants showed that 39% arose as a result of recombination between cloned A. terreus DNA sequences and yeast chromosome XII. The recombination events most likely occurred in the regions of homology within the rDNA clusters of A. terreus and Saccharomyces cerevisiae.
Shoubochkina
E A
EA
Institute of Biochemistry and Physiology of Microorganisms, USSR Academy of Sciences, Puschino-on-the Oka, Moscow.
Fodor
I I
II
D001230:Aspergillus / Q000235:genetics*; D015139:Blotting, Southern; D002874:Chromosome Mapping; D003360:Cosmids; D003433:Crosses, Genetic; D003470:Culture Media; D004271:DNA, Fungal / Q000235:genetics*; D004274:DNA, Recombinant; D015247:Deoxyribonuclease HindIII; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014170:Transformation, Genetic
D003470:Culture Media; D004271:DNA, Fungal; D004274:DNA, Recombinant; D015247:Deoxyribonuclease HindIII