title: Germline transformation with Drosophila mutant actin genes induces constitutive expression of heat shock genes.
abstract: Two Drosophila mutants KM75 and HH5, which are mutated in the act88F actin gene specific for the indirect flight muscles (IFM), synthesize heat shock proteins (hsps) constitutively in a tissue-specific manner. We have introduced cloned mutant act88F genes into a strain containing the wild-type act88F allele by P-element-mediated transformation. Flies transformed with a 4.05 kb KM75 act88F gene fragment encoding the p42 actin variant express both p42 and hsps specifically in the IFM. Using normal/mutant chimeric genes, the mutation sites of KM75 and HH5 were mapped within the sequence encoding the last 72 amino acids of actin. An in vitro mutated gene encoding a protein that lacks the 72 carboxy-terminal amino acids also induces constitutive hsp synthesis.
authors.forename: Y
authors.initials: Y
authors.lastname: Hiromi
authors.forename: H
authors.initials: H
authors.lastname: Okamoto
authors.forename: W J
authors.initials: WJ
authors.lastname: Gehring
authors.forename: Y
authors.initials: Y
authors.lastname: Hotta
mesh_terms: D000199:Actins / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D004331:Drosophila melanogaster / Q000235:genetics*; D005426:Flight, Animal; D005786:Gene Expression Regulation; D006360:Heat-Shock Proteins / Q000235:genetics*; D008970:Molecular Weight; D009132:Muscles / Q000502:physiology; D009154:Mutation; D014018:Tissue Distribution; D014170:Transformation, Genetic; D001616:beta-Galactosidase / Q000235:genetics
chemical_list: D000199:Actins; D006360:Heat-Shock Proteins; D001616:beta-Galactosidase