title: Developmental regulation of the chicken delta 1-crystallin gene: analysis by transgenesis and gene dissection.
abstract: We previously reviewed what we had learned about the regulation of the delta 1-crystallin gene through experiments using gene transfer techniques [Kondoh et al. (1986) Cell Differ. 19, 151-160]. It was concluded then that regulatory genetic elements for the lens-specific expression are associated with the delta 1-crystallin gene, and that these chicken elements properly function in mammalian cells. In the last couple of years, we have made significant progress in the understanding of lens-specific delta-crystallin expression. This is owing to success in transgenesis of mouse with the delta 1-crystallin gene and in functional dissection of the gene which led us to the discovery of an intragenic enhancer as the major determinant for lens-specific expression. In this article, we summarize these recent advances.
authors.affiliation: Department of Biophysics, Faculty of Science, University of Kyoto, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Kondoh
authors.forename: K
authors.initials: K
authors.lastname: Katoh
authors.forename: Y
authors.initials: Y
authors.lastname: Takahashi
authors.forename: K
authors.initials: K
authors.lastname: Goto
authors.forename: S
authors.initials: S
authors.lastname: Hayashi
authors.forename: T S
authors.initials: TS
authors.lastname: Okada
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D002642:Chick Embryo; D002645:Chickens; D003459:Crystallins / Q000235:genetics*; D005786:Gene Expression Regulation; D007908:Lens, Crystalline / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*
chemical_list: D003459:Crystallins