title: Evaluation of Tc-99m-DTPA for renal clearance studies in the pig.
abstract: Conventional creatinine clearance studies in the pig are complicated by difficulties with catheterisation, infection, accurate collection and active creatinine renal tubular reabsorption. We compared a single injection, 99mTc-DTPA elimination method with creatinine clearance. Nineteen pairs of GFR estimations were performed in 10 pigs: 7 in normal pigs and 12 following bile duct ligation and/or nephrectomy. Red cell and plasma protein binding of the isotope and its hepatobiliary excretion was assessed. Absolute and weight normalised endogenous creatinine clearances correlated significantly with 99mTc-DTPA elimination. 99mTc-DTPA red cell binding and hepatobiliary excretion were negligible. Thus, 99mTc-DTPA elimination is a valid indicator of changes in renal function in grouped porcine studies, particularly in the biliary obstruction model. However, isotope plasma protein binding was high in the 2 normal pigs assessed. Individual accuracy would be improved by routine protein binding correction, unless consistently low for a particular preparation.
authors.affiliation: Department of Surgery, University of Cape Town, Republic of South Africa.
authors.forename: C S
authors.initials: CS
authors.lastname: Worthley
authors.forename: M J
authors.initials: MJ
authors.lastname: Byrne
authors.forename: R
authors.initials: R
authors.lastname: Hickman
mesh_terms: D000818:Animals; D001646:Bile / Q000378:metabolism; D001798:Blood Proteins / Q000378:metabolism; D003404:Creatinine / Q000378:metabolism; D004912:Erythrocytes / Q000378:metabolism; D005260:Female; D005919:Glomerular Filtration Rate; D007668:Kidney / Q000502:physiology*; D008099:Liver / Q000378:metabolism; D009942:Organometallic Compounds; D004369:Pentetic Acid; D013552:Swine; D013667:Technetium; D016284:Technetium Tc 99m Pentetate
chemical_list: D001798:Blood Proteins; D009942:Organometallic Compounds; D013667:Technetium; D004369:Pentetic Acid; D003404:Creatinine; D016284:Technetium Tc 99m Pentetate