Effect of polyamine limitation on DNA synthesis and development of mouse preimplantation embryos in vitro.
In-vitro treatment of preimplantation mouse embryos with spermine and spermidine biosynthesis inhibitor, methylglyoxal-bis-(guanylhydrazone) (MGBG), arrested embryo development at the 8-cell or morula stage. In addition, the embryo DNA synthetic rate, as measured by [3H]thymidine incorporation, was strongly inhibited. The inhibition of blastocyst formation and DNA synthesis by MGBG was readily reversible by an exogenous supply of spermine and/or spermidine to the culture medium. DL-alpha-Methylornithine or DL-alpha-difluoromethylornithine (alpha-DFMO), inhibitors of putrescine biosynthesis, had no effect on embryos cultured for 1 or 2 days, but on the 3rd day embryo DNA synthesis was significantly depressed in the presence of alpha-DFMO. These observations suggest that, during early development of the preimplantation mouse embryo, spermine and spermidine are involved in regulation of embryo growth and DNA synthesis. They may also indicate a role of putrescine at a later stage of mouse embryo development.
Zwierzchowski
L
L
Członkowska
M
M
Guszkiewicz
A
A
D000818:Animals; D001755:Blastocyst / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D003470:Culture Media; D004247:DNA / Q000096:biosynthesis*; D000518:Eflornithine; D047108:Embryonic Development; D005260:Female; D066298:In Vitro Techniques; D051379:Mice; D008935:Mitoguazone / Q000494:pharmacology; D009952:Ornithine / Q000031:analogs & derivatives / Q000494:pharmacology; D011073:Polyamines / Q000378:metabolism*; D011247:Pregnancy; D013096:Spermine / Q000494:pharmacology
D003470:Culture Media; D011073:Polyamines; D013096:Spermine; C025025:alpha-methylornithine; D004247:DNA; D009952:Ornithine; D008935:Mitoguazone; D000518:Eflornithine