[Immunoenzyme analysis using paper disks].
Filter paper discs have been used in the enzyme immunoassay (EIA) as solid phase instead of polystyrene plates. The use of paper discs has made it possible to achieve a multiple increase in the sensitivity of sandwich EIA, thus permitting the detection of Yersinia pestis capsular antigen at a concentration of 0.4 ng/ml. Paper discs can be used not only for the sorption of antigen and antibodies, but also for the affinity purification of preparations.
Levi
M I
MI
Iagovkina
V N
VN
D000327:Adsorption; D000907:Antibodies, Bacterial / Q000032:analysis / Q000302:isolation & purification; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial / Q000032:analysis / Q000302:isolation & purification; D004912:Erythrocytes / Q000276:immunology; D005069:Evaluation Studies as Topic; D007124:Immunoenzyme Techniques / Q000295:instrumentation*; D007164:Immunosorbents; D009500:Neutralization Tests / Q000379:methods; D010209:Paper; D015010:Yersinia pestis / Q000276:immunology
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D007164:Immunosorbents
Immunofermentnyĭ analiz s ispol'zovaniem bumazhnykh diskov.