title: Role of hepatocytes in the uptake of IgA and IgA-containing immune complexes in mice.
abstract: The role of parenchymal and nonparenchymal mouse liver cells in the uptake of polymeric IgA (pIgA) and pIgA-containing immune complexes (IC) of low mol. wt (less than 1 x 10(6)) was studied. As detected by immunofluorescence and immunoelectron microscopy, pIgA were bound on the surface of isolated hepatocytes. Following the injection of radiolabeled pIgA or pIgA-IC into mice, the total radioactivity recovered from isolated liver cells was preferentially associated with parenchymal cells. The ability to inhibit the transport of pIgA and pIgA-IC by pIgA of an irrelevant specificity suggests that pIgA-IC and pIgA are bound and transported by the same mechanism. These results indicate that mouse hepatocytes are involved in the uptake and hepatobiliary transport of pIgA and pIgA-IC of low mol. wt.
authors.affiliation: Department of Microbiology, University of Alabama, Birmingham 35294.
authors.forename: J O
authors.initials: JO
authors.lastname: Phillips
authors.forename: K
authors.initials: K
authors.lastname: Komiyama
authors.forename: J M
authors.initials: JM
authors.lastname: Epps
authors.forename: M W
authors.initials: MW
authors.lastname: Russell
authors.forename: J
authors.initials: J
authors.lastname: Mestecky
mesh_terms: D000818:Animals; D000936:Antigen-Antibody Complex / Q000378:metabolism*; D001646:Bile / Q000276:immunology; D001692:Biological Transport / Q000187:drug effects; D002499:Centrifugation, Density Gradient; D003513:Cycloheximide / Q000494:pharmacology; D005455:Fluorescent Antibody Technique; D007070:Immunoglobulin A / Q000378:metabolism*; D008099:Liver / Q000276:immunology*; D008854:Microscopy, Electron; D009115:Muridae / Q000276:immunology*
chemical_list: D000936:Antigen-Antibody Complex; D007070:Immunoglobulin A; D003513:Cycloheximide