title: Spectroscopic studies on the mode of binding of ATP, UTP and alpha-amanitin with yeast RNA polymerase II.
abstract: The binding affinity between the substrates ATP and UTP with the purified yeast RNA polymerase II have been studied here in the presence and absence of Mn2+. In the absence of template DNA, both ATP and UTP showed tight binding with the enzyme without preference for any specific nucleotide, unlike Escherichia coli RNA polymerase. Fluorescence titration of the tryptophan emission of the enzyme by nucleoside triphosphate substrates gave an estimated Kd value around 65 microM in the absence of Mn2+ whereas in the presence of Mn2+, the Kd was 20 microM. The effect of substrates on the longitudinal relaxation of the HDO proton in enzyme-substrate complex also yielded a similar Kd value.
authors.affiliation: Centre for Cellular and Molecular Biology, Hyderabad, India.
authors.forename: P
authors.initials: P
authors.lastname: Bhargava
authors.forename: D
authors.initials: D
authors.lastname: Chatterji
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D000546:Amanitins / Q000378:metabolism*; D001665:Binding Sites; D007700:Kinetics; D011485:Protein Binding; D012319:RNA Polymerase II / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D013050:Spectrometry, Fluorescence; D013698:Templates, Genetic; D014364:Tryptophan / Q000032:analysis; D014500:Uracil Nucleotides / Q000378:metabolism*; D014544:Uridine Triphosphate / Q000378:metabolism*
chemical_list: D000546:Amanitins; D014500:Uracil Nucleotides; D014364:Tryptophan; D000255:Adenosine Triphosphate; D012319:RNA Polymerase II; D014544:Uridine Triphosphate