Use of cloned htpR gene of Escherichia coli to introduce htpR mutation into the chromosome.
A deletion htpR mutant of Escherichia coli has been constructed on the basis of site-directed mutagenesis. To this end, the chromosomal allele of htpR gene was substituted by a mutant allele introduced into the cell with a recombinant plasmid. The htpR mutant is characterized by a reduced level of proteolysis and therefore by a decreased rate of proteolytic degradation of RNA polymerase of bacteriophage T7. The mutation in htpR is linked with chloramphenicol resistance.
Kiselev
V I
VI
Institute of Applied Molecular Biology Ministry of Health of USSR, Moscow.
D000483:Alleles; D002702:Chloramphenicol Resistance / Q000235:genetics; D002872:Chromosome Deletion; D002876:Chromosomes, Bacterial; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics* / Q000648:ultrastructure; D005798:Genes, Bacterial; D008040:Genetic Linkage; D009154:Mutation; D010957:Plasmids