[Possibility of the existence of a pre-incision stage of DNA repair].
A study was made of the influence of protease activity inhibition by phenylmethylsulfonyl fluoride and modification of the chromatin structure by polyamines on the rate of formation and repair of DNA single-strand breaks in 3T3 cells affected by UV- or gamma-radiation. The results obtained suggest that a change in the chromatin structure, with the participation of the proteolytic activity, may be a stage preceding endonuclease incision in the process of excision DNA repair.
Ostrovskaia
L B
LB
Bezlepkin
V G
VG
Gaziev
A I
AI
D000818:Animals; D002460:Cell Line; D002843:Chromatin / Q000187:drug effects; D003037:Cobalt Radioisotopes; D004249:DNA Damage; D004260:DNA Repair; D004277:DNA, Single-Stranded / Q000528:radiation effects*; D005720:Gamma Rays; D066298:In Vitro Techniques; D051379:Mice; D010447:Peptide Hydrolases / Q000502:physiology; D010664:Phenylmethylsulfonyl Fluoride / Q000494:pharmacology; D011073:Polyamines / Q000494:pharmacology; D011480:Protease Inhibitors / Q000494:pharmacology; D014466:Ultraviolet Rays
D002843:Chromatin; D003037:Cobalt Radioisotopes; D004277:DNA, Single-Stranded; D011073:Polyamines; D011480:Protease Inhibitors; D010664:Phenylmethylsulfonyl Fluoride; D010447:Peptide Hydrolases
O vozmozhnosti sushchestvovaniia preintsizionnogo étapa reparatsii DNK.