[Involvement of cAMP dependent protein kinase in the reversion of a NIH/3T3 cell line transformed by a ras oncogene (EJ-NIH/3T3)].
cAMP production and the activity of cAMP dependent protein kinase (Kinase-A) were examined in a mutant clone of a NIH/3T3 cell line transformed by a human activated H-ras-1 oncogene (EJ-NIH/3T3). The mutant (R1) shows the characteristics of a flat revertant. The amount of cAMP increases more significantly in R1 than that in EJ-NIH/3T3 in the presence of PGE1. Enhanced activity of Kinase-A was also noted in R1 when compared to that in EJ-NIH/3T3. Further, EJ-NIH/3T3 treated with agents which increase intracellular cAMP content partially lost some characteristics of malignantly transformed phenotypes in vitro. These data suggest that Kinase-A might be involved in the reversion of EJ-NIH/3T3. In addition, reduced cytosolic free Ca2+ concentration measured with Ca2+ indicator in R1 cells was noted. This might also be associated with the reversion of the malignantly transformed cell line.
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Third Department of Internal Medicine, Hokkaido University School of Medicine, Sapporo, Japan.
D002118:Calcium / Q000378:metabolism; D002461:Cell Line, Transformed; D002471:Cell Transformation, Neoplastic / Q000378:metabolism* / Q000473:pathology; D002999:Clone Cells; D003600:Cytosol / Q000378:metabolism; D011905:Genes, ras; D006801:Humans; D011494:Protein Kinases / Q000096:biosynthesis / Q000378:metabolism*
D011494:Protein Kinases; D002118:Calcium