Characterization of a 23 kDa sperm-binding polypeptide of the golden hamster epididymis.
A 23 kDa polypeptide has been identified on the flagellum of sperm obtained from the cauda epididymis of the golden hamster. A monospecific antiserum to the 23 kDa hamster polypeptide was prepared and used to study its distribution on sperm, in the epididymis, and in epididymal fluid. In the cauda, the polypeptide is found on the midpiece and endpiece of the sperm tail, in detergent extracts of sperm, and in epididymal luminal fluid-enriched fractions. It is not present on sperm or in luminal fluid-enriched fractions from the caput epididymis. Immunocytochemical staining of epididymal tissue has demonstrated the 23 kDa polypeptide in the Golgi region of the principal cells of the proximal cauda and on sperm in the tubules of this segment and in tubules distal to it. Antiserum to the 23 kDa golden hamster polypeptide cross-reacts with sperm from rats and Chinese hamsters, but not with sperm from rabbits, cattle, mice, and guinea pigs. The antigen is localized to the tail of sperm obtained from the cauda of the rat and from the distal caput of the Chinese hamster. Immunoblots of detergent extracts of sperm and luminal fluid-enriched fractions from these two species reveal a 26 dKa polypeptide that is immunologically related to the golden hamster polypeptide.
Hoos
P C
PC
Department of Cell Biology, Vanderbilt University, Nashville, Tennessee 37232.
Olson
G E
GE
D000818:Animals; D006224:Cricetinae; D003429:Cross Reactions; D004822:Epididymis / Q000032:analysis* / Q000166:cytology; D005455:Fluorescent Antibody Technique; D007106:Immune Sera; D008297:Male; D008647:Mesocricetus; D008970:Molecular Weight; D010455:Peptides / Q000276:immunology / Q000302:isolation & purification*; D013045:Species Specificity; D013094:Spermatozoa / Q000032:analysis* / Q000166:cytology
D007106:Immune Sera; D010455:Peptides