[Effect of calcium, glucose and trifluoroperazine on the carboxymethylation of proteins in pancreatic islets of rats].
In the isolated pancreatic islets incubated in vitro, carboxylmethylation increases following stimulation with 20 mM glucose. This increase is dependent on extracellular calcium concentration and is suppressed in the presence of trifluoperazine, a calmodulin inhibitor. In conclusion, the increase in carboxylmethylation induced by 20 mM glucose seems to be a calcium-dependent process and it is probably mediated by calmodulin.
Mena
P
P
Departamento de Fisiología, Universidad de Extremadura, Badajoz, España.
Barriga
C
C
Campillo
J E
JE
D000818:Animals; D002118:Calcium / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D005947:Glucose / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D007515:Islets of Langerhans / Q000378:metabolism*; D008297:Male; D000432:Methanol / Q000032:analysis; D008745:Methylation; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D014268:Trifluoperazine / Q000494:pharmacology*
D011506:Proteins; D014268:Trifluoperazine; D005947:Glucose; D002118:Calcium; D000432:Methanol
Efecto del calcio, la glucosa y la trifluoperazina sobre la carboxilmetilación de proteínas en islotes pancreáticos de rata.