Enhanced ligation of repair sites under conditions of inhibition of poly(ADP-ribose) synthesis by 3-aminobenzamide.
The rate of intracellular ligation of excision-repair patches has been measured under conditions of inhibition of poly(ADP-ribose) synthesis by 3-aminobenzamide. Excision-repair patches in DNA of cells damaged by methyl methanesulfonate were labeled with [3H]thymidine and blocked at an intermediate stage by aphidicolin, an inhibitor of DNA polymerase alpha. Removal of [3H]thymidine and aphidicolin permitted the intracellular ligation rate to be determined by rapid digestion of [3H]-labeled 3' termini with exonuclease III. Contrary to previous conclusions from more indirect experiments, inhibition of poly(ADP-ribose) synthesis by 3-aminobenzamide actually facilitates rapid ligation.
Cleaver
J E
JE
Park
S D
SD
D016590:Aphidicolin; D001549:Benzamides / Q000494:pharmacology*; D002460:Cell Line; D004256:DNA Polymerase I / Q000037:antagonists & inhibitors; D004260:DNA Repair / Q000187:drug effects*; D004224:Diterpenes / Q000633:toxicity; D005090:Exodeoxyribonucleases; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism; D008741:Methyl Methanesulfonate / Q000633:toxicity; D009702:Nucleoside Diphosphate Sugars / Q000037:antagonists & inhibitors*; D011064:Poly Adenosine Diphosphate Ribose / Q000037:antagonists & inhibitors* / Q000096:biosynthesis
D001549:Benzamides; D004224:Diterpenes; D009702:Nucleoside Diphosphate Sugars; D011064:Poly Adenosine Diphosphate Ribose; D016590:Aphidicolin; C025160:3-aminobenzamide; D008741:Methyl Methanesulfonate; D004256:DNA Polymerase I; D005090:Exodeoxyribonucleases; C020133:exodeoxyribonuclease III