Functionally important conserved amino-acids in interferon-alpha 2 identified with analogues produced from synthetic genes.
A gene was chemically synthesised and expressed in Escherichia coli to produce [Ala30,32,33]IFN-alpha 2, an analogue of human alpha 2-interferon (IFN-alpha 2) which is devoid of activity on human cells. Eight additional analogues provided single changes in IFN-alpha 2 at each of these three conserved positions. No one residue is essential for activity, but both antiviral and anti-proliferative activity are particularly sensitive to changes in the side-chain of Arg33.
Camble
R
R
Petter
N N
NN
Trueman
P
P
Newton
C R
CR
Carr
F J
FJ
Hockney
R C
RC
Moore
V E
VE
Greene
A R
AR
Holland
D
D
Edge
M D
MD
D000596:Amino Acids / Q000032:analysis*; D004274:DNA, Recombinant; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D005796:Genes; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics / Q000502:physiology*; D010957:Plasmids
D000596:Amino Acids; D004274:DNA, Recombinant; D007371:Interferon-gamma