Characteristics of rat liver microsomal 3-hydroxy-3-methylglutaryl-coenzyme A reductase.
A procedure for the preparation of rat liver microsomal fractions essentially devoid of contaminating lysosomes is described. When this preparation was examined by immunoblotting with a rabbit antiserum to rat 3-hydroxy-3-methylglutaryl-CoA reductase, a single band corresponding to an Mr of 100000 was observed. No evidence was found for glycosylation of rat liver-3-hydroxy-3-methylglutaryl-CoA reductase. Native rat liver microsomal 3-hydroxy-3-methylglutaryl-CoA reductase differs from the purified proteolytically modified species in that it displays allosteric kinetics towards NADPH.
Ness
G C
GC
Sample
C E
CE
Smith
M
M
Pendleton
L C
LC
Eichler
D C
DC
D000118:Acetylglucosaminidase; D000818:Animals; D002458:Cell Fractionation; D004591:Electrophoresis, Polyacrylamide Gel; D006903:Hydroxymethylglutaryl CoA Reductases / Q000302:isolation & purification / Q000378:metabolism*; D025782:Hydroxymethylglutaryl-CoA-Reductases, NADP-dependent; D007122:Immunoelectrophoresis; D007700:Kinetics; D008247:Lysosomes / Q000201:enzymology; D008297:Male; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase; D008862:Microsomes, Liver / Q000201:enzymology*; D009249:NADP / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains
D009249:NADP; D006903:Hydroxymethylglutaryl CoA Reductases; D025782:Hydroxymethylglutaryl-CoA-Reductases, NADP-dependent; D000118:Acetylglucosaminidase; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase