Noncellular immunoadsorbents for preparing typing sera that recognize high-frequency red cell antigens.
The feasibility of using salivary ABH substance, covalently coupled to an affinity gel as an immunoadsorbent for anti-A and -B in sera containing antibodies to high-frequency antigens was investigated. Several examples of anti-Hy (n = 4), -Jra (n = 3), -U (n = 3), -Lub (n = 3), -Tja (n = 8), and -Vel (n = 6), plus one anti-Coa, were treated by batch adsorption with the affinity gel. Titration of the adsorbed sera with ABO-incompatible cells lacking the relevant high-frequency antigen, in parallel with ABO-compatible cells expressing the high-frequency antigen, revealed that ABO antibodies could be adsorbed to exhaustion without loss of the sera's activity against cells bearing high-frequency antigens.
Gee
S W
SW
Department of Immunology, North London Blood Transfusion Centre, Edgware, Middlesex, England.
Lubenko
A
A
D000017:ABO Blood-Group System / Q000276:immunology; D000936:Antigen-Antibody Complex; D001788:Blood Grouping and Crossmatching / Q000379:methods; D006801:Humans; D007106:Immune Sera; D007163:Immunosorbent Techniques; D007164:Immunosorbents; D007202:Indicators and Reagents; D007518:Isoantibodies / Q000032:analysis; D012463:Saliva / Q000276:immunology; D012685:Sepharose / Q000031:analogs & derivatives
D000017:ABO Blood-Group System; D000936:Antigen-Antibody Complex; D007106:Immune Sera; D007164:Immunosorbents; D007202:Indicators and Reagents; D007518:Isoantibodies; C035771:Sepharose CL 4B; D012685:Sepharose