title: Mutagenicity of red wine in the L-arabinose resistance test with Salmonella typhimurium.
abstract: The forward mutation assay to L-arabinose resistance (Ara test) in Salmonella typhimurium detected a Spanish red table wine (Rioja) as a direct-acting mutagen. The best mutagenic response was obtained by preincubating strain BA13 with the wine sample in the presence of sodium phosphate buffer and in the absence of any external metabolic activation. In fact, the S9 mixture abolished most of the mutagenic activity of red wine in the Ara test. Such an inactivating capacity seems to be independent of microsomal enzymes and mediated through some kind of heat-stable component(s) in the S9 fraction. Both regular wine (directly from the bottle) and lyophilized wine were strong mutagens in the Ara test, inducing 4914 and 2739 AraR mutants/ml. Both pKM101 and delta uvrB were critical factors in the detection of the mutagenicity of wine, exhibiting a synergic effect in strain BA13. The mutagenicity of red wine was somehow pH-dependent, increasing with the pH value of the preincubation mixture. In comparison with the Ara test, the His reverse mutation assay (Ames test) was much less sensitive to the mutagenicity of lyophilized red wine, TA102 being the most (448 His+/ml) and TA98 the least (38 His+/ml) sensitive strain. TA100, TA104 and TA97 manifested intermediate mutagenicities to red wine. Previous reports have identified strain TA98 as the His strain most sensitive to the apolar fraction (e.g. XAD-2-bound) of red wine. Based on these results we propose that TA98 mainly detects glycosides of mutagenic flavonols present in red wine (quercetin, rutin, etc.), which do not constitute the major direct-acting mutagens detected with the Ara test.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Departmento de Genética, Facultad de Ciencias, Universidad de Córdoba, Spain.
authors.forename: G
authors.initials: G
authors.lastname: Dorado
authors.forename: R R
authors.initials: RR
authors.lastname: Ariza
authors.forename: C
authors.initials: C
authors.lastname: Pueyo
mesh_terms: D001089:Arabinose / Q000494:pharmacology; D004352:Drug Resistance, Microbial; D005612:Freeze Drying; D009152:Mutagenicity Tests / Q000379:methods; D009153:Mutagens / Q000378:metabolism; D009154:Mutation; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics; D014920:Wine
chemical_list: D009153:Mutagens; D001089:Arabinose