Transport and utilization of free fatty acids in Triatoma infestans.
Triatoma infestans hemolymph has 0.31 mg/ml of free fatty acids and 2.8 mg/ml of diacylglycerols. Almost all the diacylglycerols are transported by lipophorin whereas free fatty acids are carried by lipophorin and a very high density lipoprotein. The binding of cis-parinaric acid to lipophorin was employed to specify the free fatty acid binding properties of lipophorin. Lipophorin has 10 binding sites of high affinity (3 x 10(7)) and approximately 45 binding sites of low affinity (1 x 10(6)). The relative rate of tissue incorporation of free fatty acids and diacylglycerols was measured by injecting insects with hemolymph previously labeled in both, free fatty acids and diacylglycerols. In this way, the half-life of the hemolymph free fatty acids was estimated to be about 4 min. Based on this result and taking into account the content of free fatty acids and diacylglycerols in hemolymph, the incorporation of free fatty acids, expressed in moles of fatty acids, seems to be 3.4 times higher than that of diacylglycerols. This finding can be applied to other insects.
Soulages
J L
JL
Instituto de Investigaciones Bioquímicas de La Plata, CONICET-UNLP, Facultad de Cs.Médicas, Argentina.
Rimoldi
O J
OJ
Peluffo
O R
OR
Brenner
R R
RR
D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism; D004075:Diglycerides / Q000378:metabolism; D005230:Fatty Acids, Nonesterified / Q000378:metabolism*; D006458:Hemolymph / Q000378:metabolism; D008074:Lipoproteins; D008297:Male; D014224:Triatoma / Q000378:metabolism*; D014225:Triatominae / Q000378:metabolism*
D002352:Carrier Proteins; D004075:Diglycerides; D005230:Fatty Acids, Nonesterified; D008074:Lipoproteins; C032830:lipophorin