Epidermal growth factor and insulin-like growth factor I stimulate the hydrolysis of the insulin-sensitive phosphatidylinositol-glycan in BC3H-1 myocytes.
BC3H-1 myocytes contain a phospholipid(s) which is labeled with [3H]inositol, [3H]glucosamine, and [3H]myristate [a phosphatidylinositol-glycan (PI-glycan)], and which is hydrolyzed by insulin induced activation of a specific phospholipase C. Similarly, epidermal growth factor and insulin-like growth factor-I provoke rapid increases in the hydrolysis of this PI-glycan, suggesting that derived signaling substances may be important in the action of agonists which activate tyrosine kinase type receptors.
Farese
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RV
J.A. Haley Veterans' Hospital, Tampa, FL 33612.
Nair
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GP
Standaert
M L
ML
Cooper
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DR
D000818:Animals; D002478:Cells, Cultured; D002855:Chromatography, Thin Layer; D004789:Enzyme Activation; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D005944:Glucosamine / Q000032:analysis / Q000378:metabolism; D007328:Insulin / Q000494:pharmacology*; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D009132:Muscles / Q000201:enzymology / Q000378:metabolism*; D010716:Phosphatidylinositols / Q000378:metabolism*; D011134:Polysaccharides / Q000378:metabolism*; D013002:Somatomedins / Q000494:pharmacology*; D010738:Type C Phospholipases / Q000378:metabolism
D007328:Insulin; D010716:Phosphatidylinositols; D011134:Polysaccharides; D013002:Somatomedins; D004815:Epidermal Growth Factor; D007334:Insulin-Like Growth Factor I; D010738:Type C Phospholipases; D005944:Glucosamine