Developmental expression in embryonic rat and chicken brain of a polysialoganglioside-antigen reacting with the monoclonal antibody Q 211.
The monoclonal mouse antibody Q 211 binds to an antigen, which is expressed by postmitotic growing neurons of embryonic chicken and rat brain. In chicken, thin layer chromatography (TLC) immunostaining confirms the presence of the Q 211 antigen in at least 3 different polysialoganglioside fractions. One comigrates on TLC plates with GP1c and the others with gangliosides, which have been previously preliminary characterized as GQ1c, and as a hexasialoganglioside. Thus, 3 sialic acid residues linked to the inner galactose of a complete tetraose moiety is suggested as the common epitope of the different Q 211-antigen-active gangliosides. Also in the embryonic rat brain, immunohistochemistry reveals a transient expression of the Q 211 antigen in areas containing growing nerve fibres. Unlike chicken, however, in the rat the staining is restricted to early thalamocortical innervations and to a fibre system (probably long distance projections) connecting the mamillary body with the hippocampus formation. In ganglioside extracts from rat forebrain 2 polysialogangliosides are shown by immuno-TLC to bind Q 211. One of these fractions, occurring transiently and in parallel with histochemical staining, comigrates on TLC plates with chicken GP1c. The other comigrates with the second main Q 211 antigen-containing band of chicken, which was preliminary identified as GQ1c.
Rösner
H
H
Institute of Zoology, University of Hohenheim-Stuttgart, F.R.G.
Greis
C
C
Henke-Fahle
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S
D000818:Animals; D000911:Antibodies, Monoclonal; D001921:Brain / Q000196:embryology*; D002642:Chick Embryo; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D005455:Fluorescent Antibody Technique; D005732:Gangliosides / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification; D051381:Rats; D011919:Rats, Inbred Strains
D000911:Antibodies, Monoclonal; D005732:Gangliosides