Identification of Mycoplasma gallisepticum and M. synoviae by flow cytometry.
Immunofluorescence and flow cytometric methods were examined to detect and distinguish Mycoplasma gallisepticum and M. synoviae. The procedure employed 24-hr broth cultures of each organism, direct immunofluorescence staining with either homologous or heterologous antiserum, and analyses by flow cytometry. The organisms were distinguishable on the basis of fluorescent profiles when stained with the appropriate antiserum.
May
J D
JD
U.S. Department of Agriculture, South Central Poultry Research Laboratory, Mississippi State, Mississippi 39762.
Branton
S L
SL
Cuchens
M A
MA
D002478:Cells, Cultured; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D007106:Immune Sera; D009174:Mycoplasma / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification*
D007106:Immune Sera