The lysine-49 phospholipase A2 from the venom of Agkistrodon piscivorus piscivorus. Relation of structure and function to other phospholipases A2.
A new class of phospholipases A2 that have a lysine at position 49 differ from the more conventional Asp-49 enzymes with respect to the sequential binding of the essential cofactor, calcium, and the substrate, phospholipid, in the formation of the catalytic complex (Maraganore, J.M., Merutka, G., Cho, W., Welches, W., Kézdy, F.J., and Heinrikson, R.L. (1984) J. Biol. Chem. 259, 13839-13843). We report here the complete amino acid sequence of the Lys-49 enzyme from Agkistrodon piscivorus piscivorus. The sequence was determined by automated Edman degradation of the intact, S-carboxymethylcysteinyl protein and of peptides derived therefrom by cleavage with cyanogen bromide, chymotrypsin, trypsin, and endoproteinase Lys-C. Despite several changes at amino acid residues previously considered to be invariant, the Lys-49 enzymes are homologous to the Asp-49 phospholipases. Homology is especially apparent in the following: 1) the pattern of 14 half-cystine residues, 2) conservation of hydrophobic residues which have been shown to encircle the active site, and 3) conservation of Asp-99 and His-48 which have been implicated in the catalytic reaction itself. These observations together with kinetic and binding data imply that the Lys-49 phospholipases have a catalytic mechanism and a three-dimensional architecture similar to those of the Asp-49 enzymes. Modeling of the Lys-49 enzyme based upon the structure of bovine pancreatic phospholipase reveals that the epsilon-amino group of Lys-49 can fit easily in the calcium-binding site and, moreover, that this orientation of a cationic side chain at position 49 could account for the characteristic and novel feature of the Lys-49 phospholipases, i.e. that they are able to form complexes with phospholipid in the absence of calcium.
Maraganore
J M
JM
Heinrikson
R L
RL
D000595:Amino Acid Sequence; D002118:Calcium / Q000494:pharmacology; D002850:Chromatography, Gel; D002918:Chymotrypsin; D003435:Crotalid Venoms / Q000032:analysis*; D003488:Cyanogen Bromide; D010450:Endopeptidases; D007202:Indicators and Reagents; D007700:Kinetics; D008239:Lysine; D008666:Metalloendopeptidases; D010446:Peptide Fragments / Q000302:isolation & purification; D010740:Phospholipases / Q000302:isolation & purification / Q000378:metabolism; D010741:Phospholipases A / Q000302:isolation & purification / Q000378:metabolism; D054467:Phospholipases A2; D014357:Trypsin
C031442:Agkistrodon venoms; D003435:Crotalid Venoms; D007202:Indicators and Reagents; D010446:Peptide Fragments; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2; D010450:Endopeptidases; D002918:Chymotrypsin; D014357:Trypsin; D008666:Metalloendopeptidases; C039830:peptidyl-Lys metalloendopeptidase; D008239:Lysine; D003488:Cyanogen Bromide; D002118:Calcium