Chloroperbenzoic acid induced DNA damage and peracid activation of alfatoxin B1.
The activated form of aflatoxin B1 (AFB1) in vitro and in vitro is believed to be the 2,3-oxide, which cannot be isolated, presumably due to its reactivity. In addition to in vitro activation by crude metabolic enzymes, two chemical procedures are available, one involving oxidation with the mild organic oxidant chloroperbenzoic acid and another involving the synthesis of AFB1-2,3-dichloride, an electronic analog of AFB1-2,3-oxide. Here we show that chloroperbenzoic acid by itself can modify DNA, primarily at non-basepaired adenine and guanine residues, as revealed by 'replication block' analysis.
Jacobsen
J S
JS
Humayun
M Z
MZ
D016604:Aflatoxin B1; D000348:Aflatoxins / Q000494:pharmacology; D001483:Base Sequence; D002723:Chlorobenzoates / Q000494:pharmacology; D004247:DNA; D004261:DNA Replication / Q000187:drug effects; D004277:DNA, Single-Stranded / Q000235:genetics
D000348:Aflatoxins; D002723:Chlorobenzoates; D004277:DNA, Single-Stranded; D004247:DNA; D016604:Aflatoxin B1; C000433:3-chloroperbenzoic acid