title: Assessing the specificity of anti-canine-factor VIII-related antigen.
abstract: Antibodies to canine factor VIII-related antigen should be monospecific to accurately study this protein's location in tissue and its structure. Because no uniformly accepted standard for monospecificity exists for anti-factor VIII-related antigen, we have compared the sensitivities of three common evaluation techniques--immunodiffusion, immunoelectrophoresis, and crossed immunoelectrophoresis. Unabsorbed rabbit anti-canine factor VIII-related antigen appeared monospecific against whole plasma by all three methods; however, multiple specificities were recognized against a plasma cryoconcentrate. These results demonstrate the insensitivity of the techniques under certain conditions, which can lead to erroneous interpretation of data.
authors.forename: R E
authors.initials: RE
authors.lastname: Benson
authors.forename: W J
authors.initials: WJ
authors.lastname: Dodds
mesh_terms: D000818:Animals; D000918:Antibody Specificity; D000941:Antigens / Q000276:immunology*; D004285:Dogs; D005069:Evaluation Studies as Topic; D005169:Factor VIII / Q000276:immunology*; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D007123:Immunoelectrophoresis, Two-Dimensional; D007163:Immunosorbent Techniques; D011817:Rabbits; D014841:von Willebrand Factor
chemical_list: D000941:Antigens; D014841:von Willebrand Factor; D005169:Factor VIII