Particular matrix for fast atom bombardment mass spectrometric analysis of phenolic glycolipid antigens isolated from pathogen mycobacteria.
Phenolic glycolipids play a key role as an antigenic probe for serodiagnosis of some human pathogen mycobacterial infections. The lipidic part which corresponds to a phenolphthiocerol dimycocerosate molecule, and the presence of partial O-methylated sugars, confer a high hydrophobicity to this kind of molecule. Fast atom bombardment (FAB) mass spectrometric analysis with standard matrices such as glycerol or thioglycerol was unsuccessful. Using a new matrix--monobutyltriethylene glycol--FAB analysis allows molecular weight determination and partial structural elucidation of the saccharidic and the lipidic part of those compounds.
Rivière
M
M
Centre de Recherche de Biochimie, Toulouse, France.
Fournié
J J
JJ
Vercellone
A
A
Puzo
G
G
D000942:Antigens, Bacterial / Q000032:analysis*; D006017:Glycolipids / Q000032:analysis* / Q000276:immunology; D013058:Mass Spectrometry; D009161:Mycobacterium / Q000032:analysis* / Q000276:immunology; D009166:Mycobacterium leprae / Q000032:analysis / Q000276:immunology
D000942:Antigens, Bacterial; D006017:Glycolipids