Presence of microtubules in isolated cortices of prophase I and metaphase II oocytes in Xenopus laevis.
An extensive array of microtubules has been shown to exist in the cortex of Xenopus laevis oocytes both at the prophase I and metaphase II stages. The cortical microtubules were visualized after the oocyte cortex was squashed and immunostained using anti-tubulin antibody. They are cold- and nocodazole-sensitive; their stability to both treatments decreases after meiotic maturation. Biochemical extraction of manually isolated oocyte cortices, in a microtubule-stabilizing buffer, confirms these cytological observations.
Huchon
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D
Laboratoire de Physiologie de la Reproduction, CNRS-UA 555, Université Pierre et Marie Curie, Paris, France.
Jessus
C
C
Thibier
C
C
Ozon
R
R
D000818:Animals; D001562:Benzimidazoles / Q000494:pharmacology; D003080:Cold Temperature; D003593:Cytoplasm / Q000276:immunology / Q000648:ultrastructure; D005260:Female; D005455:Fluorescent Antibody Technique; D008677:Metaphase; D008870:Microtubules / Q000187:drug effects / Q000276:immunology / Q000648:ultrastructure*; D015739:Nocodazole; D009865:Oocytes / Q000187:drug effects / Q000648:ultrastructure*; D011418:Prophase; D014404:Tubulin / Q000276:immunology; D014982:Xenopus laevis
D001562:Benzimidazoles; D014404:Tubulin; D015739:Nocodazole