Isolation of the amyloid P component from the Engelbreth-Holm-Swarm (EHS) tumor of the mouse.
The amyloid P component was isolated from the mouse EHS tumor, a producer of basement membrane-like material. Following collagenase treatment of the tissue homogenate and centrifugation, the supernatant was purified by calcium-dependent binding to agarose, and elution with ethylenediaminetetraacetic acid. Identification of the purified material as the amyloid P component was established by immunodiffusion and electron microscopic appearance as 8.5 nm pentagonal units, frequently assembled into columns. SDS-PAGE gel electrophoresis yielded 25,000 D bands, suggesting that the amyloid P is of the mouse type. It is proposed that the mouse amyloid P component extracted from the tumor is located within the basotubules present in the pericellular matrix.
Inoue
S
S
Skinner
M
M
Leblond
C P
CP
Shirahama
T
T
Cohen
A S
AS
D000682:Amyloid / Q000276:immunology / Q000302:isolation & purification*; D000818:Animals; D005779:Immunodiffusion; D051379:Mice; D008854:Microscopy, Electron; D008970:Molecular Weight; D009374:Neoplasms, Experimental / Q000032:analysis*; D000683:Serum Amyloid P-Component
D000682:Amyloid; D000683:Serum Amyloid P-Component