Phospholipase C activity in normal rat mammary tissues and in DMBA-induced rat mammary tumors.
Employing phosphatidylinositol as the substrate, phospholipase C (PLC) activity was measured in various cellular fractions derived from DMBA-induced rat mammary tumors and mammary tissues from 12-14 day pregnant rats. In the 2,000g pellet, 10,000g pellet, 100,000g pellet and 100,000g supernatant fractions, PLC activity was more than 5 fold higher in the fractions derived from the neoplastic tissues. This was true whether PLC activity was expressed on the basis of protein content or 5' nucleotidase activities.
Rillema
J A
JA
D015127:9,10-Dimethyl-1,2-benzanthracene; D000818:Animals; D005260:Female; D008321:Mammary Glands, Animal / Q000201:enzymology*; D008325:Mammary Neoplasms, Experimental / Q000139:chemically induced / Q000201:enzymology*; D011247:Pregnancy; D051381:Rats; D011919:Rats, Inbred Strains; D010738:Type C Phospholipases / Q000378:metabolism*
D015127:9,10-Dimethyl-1,2-benzanthracene; D010738:Type C Phospholipases