Studies on Saccharomyces cerevisiae carrying the plasmid pCYG4 related with ammonia assimilation. Batch experiments.
Batch culture experiments of three different strains of Saccharomyces cerevisiae have been carried out. The first strain was transformed by a plasmid pCYG4, which carries the glutamate dehydrogenase (NADP-GDH, E.C. 1.4.14) gene conferring an 11-fold increase in activity. The second was transformed by the same plasmid, but without NADP-GDH, and the third was the wild type. The specific growth rates of the two recombinant DNA strains were below that of the wild type, which can be related to extra plasmid protein production.
Lima Filho
J L
JL
Departamento de Bioquimica, Universidade Federal de Pernambuco, Cidade Universitaria, Recife-PE, Brasil.
Ledingham
W M
WM
D000641:Ammonia / Q000378:metabolism*; D003470:Culture Media; D005969:Glutamate Dehydrogenase / Q000235:genetics; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism
D003470:Culture Media; D000641:Ammonia; D005969:Glutamate Dehydrogenase