Chemiluminescence of canine peripheral blood lymphocytes stimulated by mitogens.
Luminol-dependent chemiluminescence of peripheral blood lymphocytes from dogs stimulated with concanavalin A (Con A) or phytohemagglutinin P (PHA) was measured with a Pico-Lite luminometer. 10 microliter of luminol gave optimal quantum yield from 1 X 10(6) lymphocytes sensitized with either 80 micrograms Con A or 160 micrograms PHA. Addition of superoxide dismutase did not influence the course of chemiluminescence. Whereas catalase produced 41% increase in quantum yield, mannitol caused a 51% inhibition of chemiluminescence. Lymphocytes exposed to varying doses of short term x-irradiation or lymphocytes isolated from dogs kept under continuous exposure through a gamma irradiation source showed dose-related depression of chemiluminescence. Membrane factors may be involved in lymphocyte stimulation to chemiluminescence as pulse experiments with Con A and PHA revealed. It is proposed that chemiluminescence measurements may be useful in monitoring early events in lymphocyte stimulation by antigens and mitogens.
Ekejindu
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Misra
H P
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D000818:Animals; D002374:Catalase / Q000494:pharmacology; D003208:Concanavalin A / Q000494:pharmacology; D004285:Dogs; D005720:Gamma Rays; D008163:Luminescent Measurements; D008165:Luminol / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000187:drug effects / Q000528:radiation effects; D008353:Mannitol / Q000494:pharmacology; D010777:Photochemistry; D010835:Phytohemagglutinins / Q000494:pharmacology; D011724:Pyridazines / Q000494:pharmacology*; D011975:Receptors, Mitogen / Q000032:analysis; D013482:Superoxide Dismutase / Q000494:pharmacology
D010835:Phytohemagglutinins; D011724:Pyridazines; D011975:Receptors, Mitogen; D003208:Concanavalin A; D008353:Mannitol; D008165:Luminol; D002374:Catalase; D013482:Superoxide Dismutase