Somatic deletions and mutations of c-Ha-ras gene in human cervical cancers.
The c-Ha-ras-1 locus was analysed in cervical cancers and shown to exhibit the loss of one allele in 36% of heterozygous tumours and a mutation at codon 12 in 24% of tumours at advanced stages. 40% of tumours with mutation contained also a deletion. A c-myc gene activation was found in 100% and 70% of tumours containing mutation and deletion respectively. This suggests that the two proto-oncogenes cooperate for the progression of cervical cancers. Furthermore as more than 90% of these tumours contained also human papillomavirus sequences, our data strongly suggest that multiple genetic events are involved in the genesis and progression of most cervical cancers.
Riou
G
G
Laboratoire de Pharmacologie Clinique et Moléculaire, Institut Gustave Roussy, Villejuif, France.
Barrois
M
M
Sheng
Z M
ZM
Duvillard
P
P
Lhomme
C
C
D000483:Alleles; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D002872:Chromosome Deletion; D003062:Codon; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D006579:Heterozygote; D006801:Humans; D009154:Mutation; D009363:Neoplasm Proteins / Q000235:genetics; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D016271:Proto-Oncogene Proteins c-myc; D016283:Proto-Oncogene Proteins p21(ras); D011519:Proto-Oncogenes; D015183:Restriction Mapping; D002583:Uterine Cervical Neoplasms / Q000235:genetics*
D003062:Codon; D004273:DNA, Neoplasm; D009363:Neoplasm Proteins; D011518:Proto-Oncogene Proteins; D016271:Proto-Oncogene Proteins c-myc; C501009:HRAS protein, human; D016283:Proto-Oncogene Proteins p21(ras)