title: Experimental scoliosis in the rat. I. Methodology, anatomic features and neurologic characterization.
abstract: The purpose of this study was to develop a simple and reproducible model of scoliosis in the rat without producing direct trauma to the spine, in order to both provide a system for the study of human scoliosis and to ultimately study the conditions under which distraction-induced trauma might occur. Right lateral curvatures were produced in three groups of rats (N = 36, 59, 25) at 18-21 days of age by suturing the inferior angle of the scapula to the ipsilateral bony pelvis. In the first group of animals, it was found that a minimum period of 6 weeks of tethering was required in order to produce permanent structural curvatures after release of the tethering sutures. In the second group of rats, a number of morphologic and histologic changes characteristic of human scoliosis were noted, including apical wedging, deviation of the apical spinous process, pelvic asymmetry, rib hump deformity, vertebral rotation, displacement of the nucleus pulposus, disorganized columnization of disc cartilage cells, and an increased number of Type I muscle fibers in paravertebral muscle on the convex side of the curve. Finally, in the third group of rats, a mild spasticity was seen in the ipsilateral hindlimb in approximately one-third of the animals with curvatures greater than 40 degrees. This spasticity was not associated with a change of latency or amplitude of the somatosensory-evoked potential (SSEP). Each curvature group displayed SSEP characteristics that were not statistically different from unoperated control animals. These results indicate the validity of this model system for the study of scoliosis, and, in particular, its usefulness for the study of operative-induced trauma.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Research Department, Alfred I. duPont Institute, Wilmington, Delaware.
authors.forename: J F
authors.initials: JF
authors.lastname: Sarwark
authors.forename: K W
authors.initials: KW
authors.lastname: Dabney
authors.forename: S K
authors.initials: SK
authors.lastname: Salzman
authors.forename: T
authors.initials: T
authors.lastname: Wakabayashi
authors.forename: H K
authors.initials: HK
authors.lastname: Kitadai
authors.forename: J T
authors.initials: JT
authors.lastname: Beauchamp
authors.forename: A L
authors.initials: AL
authors.lastname: Beckman
authors.forename: W P
authors.initials: WP
authors.lastname: Bunnell
mesh_terms: D000818:Animals; D001706:Biopsy; D004195:Disease Models, Animal; D005073:Evoked Potentials, Somatosensory; D008297:Male; D009128:Muscle Spasticity / Q000209:etiology; D009132:Muscles / Q000473:pathology; D009420:Nervous System / Q000503:physiopathology*; D011859:Radiography; D051381:Rats; D011919:Rats, Inbred Strains; D012600:Scoliosis / Q000209:etiology* / Q000473:pathology / Q000503:physiopathology; D013131:Spine / Q000000981:diagnostic imaging; D013536:Suture Techniques