title: Factors influencing the expression of dental extracellular matrix biomineralization.
abstract: The forming tooth organ provides a number of opportunities to investigate the cellular and molecular biology of cell-mediated extracellular matrix (ECM) biomineralization. Regulatory processes associated with tooth formation are being investigated by identifying when and where cell adhesion molecules (CAMs), substrate adhesion molecules (SAMs), dentine phosphoprotein and enamel gene products are expressed during sequential developmental stages. In vitro organotypic culture studies in serumless, chemically-defined medium, have shown that instructive and permissive signalling are required for both morphogenesis and cytodifferentiation. Intrinsic developmental instructions (autocrine and paracrine factors) act independently of long-range hormonal or exogenous growth factors and mediate morphogenesis from the initiation of the dental lamina to the crown stages of tooth development. This review summarizes the results of studies using experimental embryology, recombinant DNA technology and immunocytology to elucidate mechanisms responsive to instructive epithelial-mesenchymal interactions associated with ameloblast differentiation, odontoblast differentiation, and dentine and enamel ECM biomineralization.
authors.affiliation: Department of Basic Sciences (Biochemistry), School of Dentistry, University of Southern California, Los Angeles 90089-0181.
authors.forename: H C
authors.initials: HC
authors.lastname: Slavkin
authors.forename: M L
authors.initials: ML
authors.lastname: Snead
authors.forename: M
authors.initials: M
authors.lastname: Zeichner-David
authors.forename: M
authors.initials: M
authors.lastname: MacDougall
authors.forename: A
authors.initials: A
authors.lastname: Fincham
authors.forename: E C
authors.initials: EC
authors.lastname: Lau
authors.forename: W
authors.initials: W
authors.lastname: Luo
authors.forename: M
authors.initials: M
authors.lastname: Nakamura
authors.forename: P
authors.initials: P
authors.lastname: Oliver
authors.forename: J
authors.initials: J
authors.lastname: Evans
mesh_terms: D000818:Animals; D005109:Extracellular Matrix / Q000502:physiology*; D006801:Humans; D008963:Molar / Q000196:embryology; D009805:Odontogenesis; D014083:Tooth Germ / Q000502:physiology