title: Lactate observation in vivo by spectral editing in real time.
abstract: We have developed a novel in vivo proton MR spectroscopy magnetization transfer method for detection of lactate in ischemic tissue in the presence of interfering fat proton resonances. Pyruvate is magnetically labeled with a saturation pulse and, when converted to lactate, the lactate retains the label. Difference of spectra obtained with and without a saturation pulse contain no fat resonances. High-resolution spectra (determined with a GE 1.5 T Signa) of low lactate levels were obtained in vivo by water suppression using a 2662 composite RF pulse and slice-selective gradients. Spectral subtraction was performed in real time allowing the monitoring of a buildup of the intensity of the lactate peak. Pyruvate-lactate saturation transfer techniques should find wide applicability in the study of ischemia.
authors.affiliation: Department of Radiology, Thomas Jefferson University Hospital, Philadelphia, PA 19107.
authors.forename: S
authors.initials: S
authors.lastname: Vinitski
authors.forename: R H
authors.initials: RH
authors.lastname: Griffey
authors.forename: J
authors.initials: J
authors.lastname: Szumowski
authors.forename: N A
authors.initials: NA
authors.lastname: Matwiyoff
mesh_terms: D006801:Humans; D007511:Ischemia / Q000378:metabolism; D007773:Lactates / Q000378:metabolism*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008961:Models, Structural; D011773:Pyruvates; D013382:Subtraction Technique
chemical_list: D007773:Lactates; D011773:Pyruvates