title: Molecular cloning, germ-line transformation, and transcriptional analysis of the zeste locus of Drosophila melanogaster.
abstract: Approximately 170 kilobase pairs (kb) of contiguous DNA sequences derived from bands 3A3,4 of the Drosophila melanogaster X chromosome have been isolated by molecular cloning. Sequences required for the wild-type expression of the zeste locus are located within a 6-kb fragment of this chromosomal region, as shown by phenotypic rescue of zeste mutants in P element-mediated germ-line transformation. Expression of zeste is correlated with a 2.2-kb poly(A)+ RNA species transcribed at all postzygotic stages of Drosophila development. Many zeste alleles, including several producing neomorphic phenotypes, are not associated with detectable rearrangements of DNA.
authors.forename: P H
authors.initials: PH
authors.lastname: Gunaratne
authors.forename: A
authors.initials: A
authors.lastname: Mansukhani
authors.forename: S E
authors.initials: SE
authors.lastname: Lipari
authors.forename: H C
authors.initials: HC
authors.lastname: Liou
authors.forename: D W
authors.initials: DW
authors.lastname: Martindale
authors.forename: M L
authors.initials: ML
authors.lastname: Goldberg
mesh_terms: D000818:Animals; D001483:Base Sequence; D002869:Chromosome Aberrations; D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification; D004331:Drosophila melanogaster / Q000033:anatomy & histology / Q000235:genetics*; D005260:Female; D008297:Male; D010641:Phenotype; D014158:Transcription, Genetic; D014170:Transformation, Genetic; D014960:X Chromosome
chemical_list: D004247:DNA