Enhancement of luminol-based immunodot and Western blotting assays by iodophenol.
The application of 4-iodophenol as a signal enhancer for luminol-based immunodot and Western blotting assays was investigated. Immunodetection of biotin-bovine serum albumin (BSA) using a rat anti-biotin antibody for immunodot binding and goat anti-biotin peroxidase conjugate for Western blotting assays was employed to demonstrate the signal enhancement attainable with 4-iodophenol. The addition of 4-iodophenol at a final working concentration of 52.2 microM to a light-emitting substrate, luminol, resulted in 3-fold and 10-fold signal enhancements for Western blotting and immunodot binding detection of biotin-BSA, respectively.
Leong
M M
MM
Department of Biology, University of Victoria, British Columbia, Canada.
Fox
G R
GR
D000818:Animals; D001710:Biotin / Q000032:analysis; D015153:Blotting, Western / Q000379:methods*; D002417:Cattle; D006041:Goats; D007158:Immunologic Techniques; D007462:Iodobenzenes; D008165:Luminol; D011724:Pyridazines; D051381:Rats; D012710:Serum Albumin, Bovine / Q000032:analysis
D007462:Iodobenzenes; D011724:Pyridazines; D012710:Serum Albumin, Bovine; C050181:4-iodophenol; D008165:Luminol; D001710:Biotin