title: Assay of total complex and activity state of branched-chain alpha-keto acid dehydrogenase complex and of activator protein in mitochondria, cells, and tissues.
authors.forename: P A
authors.initials: PA
authors.lastname: Patston
authors.forename: J
authors.initials: J
authors.lastname: Espinal
authors.forename: M
authors.initials: M
authors.lastname: Beggs
authors.forename: P J
authors.initials: PJ
authors.lastname: Randle
mesh_terms: D042942:3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide); D000818:Animals; D002250:Carbon Radioisotopes; D002417:Cattle; D004789:Enzyme Activation; D007658:Ketone Oxidoreductases / Q000032:analysis* / Q000378:metabolism; D007668:Kidney / Q000201:enzymology; D007700:Kinetics; D008928:Mitochondria / Q000201:enzymology*; D008929:Mitochondria, Heart / Q000201:enzymology; D008930:Mitochondria, Liver / Q000201:enzymology; D008931:Mitochondria, Muscle / Q000201:enzymology; D009097:Multienzyme Complexes / Q000032:analysis* / Q000378:metabolism; D011506:Proteins / Q000032:analysis* / Q000378:metabolism; D011865:Radioisotope Dilution Technique; D051381:Rats; D013053:Spectrophotometry / Q000379:methods; D013379:Substrate Specificity
chemical_list: D002250:Carbon Radioisotopes; D009097:Multienzyme Complexes; D011506:Proteins; D007658:Ketone Oxidoreductases; D042942:3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide)