[The appearance of specific IgG and IgA antibodies to Streptococcus pneumoniae in serum and in lung lavage fluid of mice after the administration of different antigens. 1. Development of an enzyme immunoassay (EIA) for the detection of specific IgG and IgA antibodies to Streptococcus pneumoniae].
Immunization against streptococcus pneumoniae is for the prophylaxis of pneumococcus diseases in an increased number of risk patients and because of an increasing presence of antibiotica resistance in present days necessary. In order to get better possibilities to test the immunization effect, improved methods for detection of antibodies are applied. In this publication a double sandwich-EIA and a protein-coupled EIA are presented and compared. The double sandwich-EIA has proved to be superior with regard to its day by day variability of 12.5% and its series variability of 3.5% and to be of the same value compared with international developments. The production of most of antigens and antisera is described and the application of gamma-irradiated micro-plates is proposed.
Schumann
V
V
Innere Abteilung I des St. Hedwig-Krankenhauses Berlin, DDR.
Tischner
H
H
Noack
K
K
Schröder
R
R
Pohl
W D
WD
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis*; D000942:Antigens, Bacterial / Q000008:administration & dosage / Q000276:immunology*; D005260:Female; D007114:Immunization; D007124:Immunoenzyme Techniques; D007070:Immunoglobulin A / Q000032:analysis*; D007074:Immunoglobulin G / Q000032:analysis*; D051379:Mice; D011817:Rabbits; D013296:Streptococcus pneumoniae / Q000276:immunology*
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D007070:Immunoglobulin A; D007074:Immunoglobulin G
Auftreten von spezifischen IgG- und IgA-Antikörpern gegen Streptococcus pneumoniae im Serum und in der Lungenspülflüssigkeit von Mäusen nach unterschiedlicher Antigenapplikation. 1. Mitteilung: Aufbau eines Enzymimmunoassay (EIA) zum Nachweis von spezifischen IgG- and IgA-Antikörpern gegen Streptococcus pneumoniae.