[Cytosine DNA-methylase of a SsoII-type from Shigella sonnei 47 cells].
Shigella sonnei 47 cells were found to contain DNA-methylase SsoII which is a modifying component of the system of host specificity of SsoII. The recognition sequence (RS) of methylase SsoII is represented by a five-member palyndromic structure--5'...CCNGG...3'--with a degenerated central nucleotide. Modification of SsoII affords protection of acceptor DNA not only from SsoII type restriction, but also from other restrictases, e. g., Eco RII having an analogous RS but with a less degenerated central nucleotide pair. A simple and rapid procedure for isolation and purification of DNA-methylase ScoII, which employs hydrophobic chromatography on phenyl-Sepharose, has been developed. The enzyme preparation does not contain trace amounts of specific and nonspecific endonucleases and keeps stable on storage in 30% glycerol over a period of one year.
Lopatina
N G
NG
Lopareva
E N
EN
Posypanova
A M
AM
Nikol'skaia
I I
II
Debov
S S
SS
D015257:DNA-Cytosine Methylases / Q000302:isolation & purification*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000302:isolation & purification*; D007525:Isoelectric Focusing; D012764:Shigella sonnei / Q000201:enzymology*
D015257:DNA-Cytosine Methylases; C045297:CCNGG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific
Tsitozinovaia DNK-metilaza SsoII-tipa iz kletok Shigella sonnei 47.