title: Effect of recombinant human granulocyte-macrophage colony-stimulating factor on the hemopoietic progenitor cells from patients with AIDS.
abstract: Derangement of the hemopoietic progenitor cells has been observed in the majority of patients with the acquired immunodeficiency syndrome (AIDS). In this study the effect of recombinant human GM-CSF was evaluated for the in vitro growth of hemopoietic progenitor cells from the bone marrow of patients with AIDS. A significant reduction of growth (mean +/- SEM) of CFU-GEMM (2.5 +/- 1.2), BFU-E (23 +/- 9), and CFU-GM (55 +/- 21) was found in AIDS patients in comparison to normal controls. There was a linear correlation between the number of CFU-GM growing in vitro after stimulation with rh GM-CSF as compared to medium conditioned by phytohemagglutinin-stimulated leukocytes (PHA-LCM). However, CFU-GM from patients with AIDS appear to need higher concentrations of rh GM-CSF for maximal growth.
authors.affiliation: Dept. of Hematology, Johann Wolfgang Goethe University, Frankfurt/Main, W.-Germany.
authors.forename: A
authors.initials: A
authors.lastname: Ganser
authors.forename: B
authors.initials: B
authors.lastname: Völkers
authors.forename: J
authors.initials: J
authors.lastname: Greher
authors.forename: D
authors.initials: D
authors.lastname: Hoelzer
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000473:pathology*; D001853:Bone Marrow / Q000473:pathology; D002455:Cell Division; D002478:Cells, Cultured; D003115:Colony-Stimulating Factors / Q000502:physiology*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D006133:Growth Substances / Q000502:physiology*; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000187:drug effects; D006801:Humans; D011994:Recombinant Proteins / Q000494:pharmacology*; D012016:Reference Values
chemical_list: D003115:Colony-Stimulating Factors; D006133:Growth Substances; D011994:Recombinant Proteins; D016178:Granulocyte-Macrophage Colony-Stimulating Factor