title: Detection of Chlamydia trachomatis by isolation in cell culture and enzyme amplified immunoassay.
abstract: 188 (139 female; 49 male) genital swabs from patients with urogenital complaints (greater than 6 weeks), 69 (33 female; 36 male) conjunctival swabs from patients with chronic conjunctivitis and 14 swabs from newborns with acute conjunctivitis were tested for the presence of Chlamydia trachomatis (Ct) by inoculation in cell culture and visualisation by indirect immunofluorescence (IFT) with a monoclonal antibody and enzyme immunoassay (ELISA). Of the 271 specimens 20 (7.4%) were Ct positive by cell culture (IFT) and 18 (6.6%) were positive by ELISA. In 184 (97.9%) urogenital specimens results between cell culture (IFT) and ELISA agreed. With ELISA 4 further genital specimens were found to be Ct positive. In 59 (85.5%) conjunctival swabs of patients with chronic conjunctivitis results between cell culture (IFT) and ELISA agreed. By inoculation of cell culture (IFT) 6 (8.7%) more Ct positive specimens could be identified which were negative in ELISA. There was complete agreement between positive and negative Ct detection with cell culture (IFT) and ELISA in the cases of acute conjunctivitis.
authors.affiliation: Max von Pettenkofer-Institut für Hygiene und Medizinische Mikrobiologie der Ludwig-Maximilians-Universität, München.
authors.forename: T F
authors.initials: TF
authors.lastname: Schwarz
authors.forename: G
authors.initials: G
authors.lastname: Zoulek
authors.forename: M
authors.initials: M
authors.lastname: Roggendorf
mesh_terms: D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D002460:Cell Line; D002690:Chlamydia Infections / Q000175:diagnosis*; D002692:Chlamydia trachomatis / Q000302:isolation & purification*; D003235:Conjunctivitis, Inclusion / Q000175:diagnosis*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005455:Fluorescent Antibody Technique; D005831:Genital Diseases, Female / Q000175:diagnosis*; D005832:Genital Diseases, Male / Q000175:diagnosis*; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D008875:Middle Aged