Experimental mumps labyrinthitis in monkeys (Macaca irus)--immunohistochemical and ultrastructural studies.
Three monkeys (Macaca irus) were inoculated with mumps virus into unilateral cochleas and their inner ear were examined by immunofluorescent microscopy and transmission electronmicroscopy. The temporal bones were removed after survival period of 14 days when serological tests disclosed elevation of anti-mumps antibody titers. Immunofluorescent microscopy revealed that the viral antigen was positive in the stria vascularis. The ultrastructural study revealed that the pathologic changes in the cochleas were marked in the organ of Corti and stria vascularis. The outer hair cells were more susceptible to the infection than the inner hair cells. In the stria vascularis, both marginal and intermediate cells were affected. It was possible to find some of marginal cells in the basal turn shedding a large number of mature virions into the endolymph. These pathologic changes observed in the cochleas of the monkeys were similar to those previously revealed in the guinea pig cochleas and thus were considered as the specific features of acute mumps labyrinthitis.
Tanaka
K
K
Department of Otolaryngology, Hokkaido University School of Medicine, Sapporo, Japan.
Fukuda
S
S
Terayama
Y
Y
Toriyama
M
M
Ishidoya
J
J
Ito
Y
Y
Sugiura
A
A
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D003051:Cochlea / Q000382:microbiology / Q000648:ultrastructure*; D004195:Disease Models, Animal; D005455:Fluorescent Antibody Technique; D006198:Hair Cells, Auditory / Q000648:ultrastructure; D007759:Labyrinth Diseases / Q000473:pathology*; D007762:Labyrinthitis / Q000382:microbiology / Q000473:pathology*; D008252:Macaca fascicularis; D009107:Mumps / Q000150:complications* / Q000473:pathology; D009925:Organ of Corti / Q000648:ultrastructure; D013316:Stria Vascularis / Q000648:ultrastructure
D000956:Antigens, Viral