The use of albumin-gold to follow lysosome-phagosome fusion.
A new procedure for labeling of secondary lysosomes and to determine, by transmission electron microscopy, their fusion with phagosomes was developed. It is based on the use of albumin adsorbed to colloidal gold particles as a probe and tested using macrophages previously labeled with albumin-gold and then incubated in the presence of epimastigote forms of Trypanosoma cruzi.
De Carvalho
T U
TU
Laboratório de Ultraestrutura Celular e Microscopia Eletrônica, Universidade Federal do Rio de Janeiro, Brasil.
Souto-Padrón
T
T
De Souza
W
W
D000418:Albumins / Q000378:metabolism; D000818:Animals; D006046:Gold / Q000378:metabolism; D007150:Immunohistochemistry; D008247:Lysosomes / Q000502:physiology / Q000648:ultrastructure*; D008264:Macrophages / Q000502:physiology / Q000648:ultrastructure; D051379:Mice; D010588:Phagosomes / Q000502:physiology / Q000648:ultrastructure*; D014349:Trypanosoma cruzi / Q000502:physiology*
D000418:Albumins; D006046:Gold