Microcomputer-based image analysis systems for two-dimensional electrophoresis gels.
The intent of this overview is to provide the readers, especially those who are currently conducting two-dimentional electrophoresis, a basic understanding in the construction and use of microcomputer-based systems for the analysis of protein profiles generated by two-dimensional gel electrophoresis. In addition, a microcomputer-based system, employing fixed-point operations and effective algorithms, has been evaluated. The validity of this system has been demonstrated by using the two-dimensional silver-stained gels and fluorograms derived from the rat prostate. It is concluded that the present system can be used to aid the analysis of two-dimensional electrophoresis gels. An overall consideration of hardware and software components of a computer-based system is briefly discussed.
Lee
C
C
Northwestern University Medical School.
Hu
S E
SE
Lok
M S
MS
Chen
Y C
YC
Tseng
C C
CC
D003199:Computer Systems; D004591:Electrophoresis, Polyacrylamide Gel; D005069:Evaluation Studies as Topic; D007091:Image Processing, Computer-Assisted / Q000295:instrumentation*; D008838:Microcomputers; D011506:Proteins / Q000302:isolation & purification*
D011506:Proteins