[Energetic parameters as a reflection of transition states in batch cultures of Escherichia coli with addition of substrate].
The physiology of Escherichia coli feed batch cultures grown in a mineral medium with glucose was studied by analysing the intracellular content of adenylate nucleotides, the respiration rate, and the calculated economic coefficients of substrate and oxygen utilisation (Yx/s and YO2) which were compared with the growth parameters. The cultures were characterised by a rapid change of the transitional states associated with substrate exhaustion, limited oxygen supply, incomplete growth synchronisation and metabolite poisoning. The transitional states determined the oscillatory mode of the constructive and energetic parameters whose comparison allowed one to reveal the periods of incomplete coupling between reactions which generated and consumed the energy.
Tkachenko
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AG
D000227:Adenine Nucleotides / Q000378:metabolism; D004734:Energy Metabolism; D004926:Escherichia coli / Q000254:growth & development / Q000378:metabolism*; D010101:Oxygen Consumption
D000227:Adenine Nucleotides
Energeticheskie parametry kak otrazhenie perekhodnykh sostoianiĭ v peiodicheskikh kul'turakh Escherichia coli s dobavleniem substrata.