The in-vitro degradation at 37 degrees C of vancomycin in serum, CAPD fluid and phosphate-buffered saline.
Solutions of vancomycin in phosphate-buffered saline, peritoneal dialysis effluent fluid and human serum were incubated at 37 degrees C for ten days and sampled at daily intervals. The samples were assayed for vancomycin content by a microbiological assay, HPLC and polarisation fluoroimmunoassay (Abbott TDX). The results obtained by HPLC and microbiological assay agreed well and indicated approximately 50% loss over ten days in serum and buffered saline and over 70% loss in dialysate. TDX results indicated losses of only 20% and 40%, respectively. Degradation products were prepared from vancomycin by acid hydrolysis and purified by HPLC. These purified products were shown to cross-react in the TDX assay. It is suggested that the TDX assay becomes non-specific in the presence of vancomycin breakdown products and thus overestimates true vancomycin concentrations.
White
L O
LO
Department of Medical Microbiology, Southmead Hospital, Bristol, UK.
Edwards
R
R
Holt
H A
HA
Lovering
A M
AM
Finch
R G
RG
Reeves
D S
DS
D001431:Bacteriological Techniques; D002851:Chromatography, High Pressure Liquid; D015200:Fluoroimmunoassay; D006801:Humans; D010531:Peritoneal Dialysis, Continuous Ambulatory; D012965:Sodium Chloride; D014640:Vancomycin / Q000097:blood / Q000378:metabolism*
D012965:Sodium Chloride; D014640:Vancomycin