Bidirectional transfer from a 24 bp border repeat of Agrobacterium tumefaciens.
T-region transfer from wild-type Agrobacterium strains is thought to be an orientated process, starting at the right border repeat and terminating at the left border repeat of the T-region. Here we demonstrate that a right border repeat in the inverted orientation relative to the onc-genes can also mediate transfer of the T-region to the plant cell, although with lower efficiency as a border repeat in the native orientation. Transfer mediated by an inverted right border repeat is stimulated by the presence of the T-region transfer enhancer. Similar single stranded molecules, comprising the bottom strand of the T-DNA, were isolated from acetosyringone induced bacteria, irrespective of the orientation of the right border. These findings show that border repeats work bidirectionally to some extent.
van Haaren
M J
MJ
Department of Plant Molecular Biology, Leiden University, The Netherlands.
Sedee
N J
NJ
de Boer
H A
HA
Schilperoort
R A
RA
Hooykaas
P J
PJ
D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics* / Q000302:isolation & purification; D004277:DNA, Single-Stranded / Q000235:genetics* / Q000302:isolation & purification; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors; D010957:Plasmids; D012091:Repetitive Sequences, Nucleic Acid; D012231:Rhizobium / Q000235:genetics*
D004269:DNA, Bacterial; D004277:DNA, Single-Stranded