Two cellular components, PrlA and SecB, that recognize different sequence determinants are required for efficient protein export.
We exploited the conditional-lethal phenotype of secB null mutations to demonstrate that SecB function was required for PrlA-mediated suppression of signal sequence mutations. The results of these experiments provide information about the functions performed and the sequence determinants recognized by each of these components of the protein export machinery of Escherichia coli.
Trun
N J
NJ
Department of Biology, Princeton University, New Jersey 08544.
Stader
J
J
Lupas
A
A
Kumamoto
C
C
Silhavy
T J
TJ
D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D029968:Escherichia coli Proteins; D005796:Genes; D005798:Genes, Bacterial; D009154:Mutation; D010641:Phenotype; D018272:Porins; D011991:Receptors, Virus / Q000235:genetics; D000069816:SEC Translocation Channels
D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D018272:Porins; D011991:Receptors, Virus; D000069816:SEC Translocation Channels; C057842:SecB protein, Bacteria; C057841:SecY protein, E coli; C016532:maltoporins