Quantitative determination of eicosanoids in biological material.
Radioimmunoassay is the most widely used method for determination of eicosanoids in biological material. Although this technique is, in principle, simple, radioimmunological data generally require extensive validation procedures. Here some examples for such methods are presented with special emphasis on the determination of cysteinyl-leukotrienes.
Peskar
B A
BA
Dept. of Pharmacology and Toxicology, Ruhr-University, Bochum, FRG.
Simmet
T
T
Peskar
B M
BM
Dembińska-Kieć
A
A
D000818:Animals; D001095:Arachidonic Acids / Q000032:analysis*; D006801:Humans; D011453:Prostaglandins / Q000032:analysis*; D011863:Radioimmunoassay / Q000379:methods
D001095:Arachidonic Acids; D011453:Prostaglandins