Random cloning of bent DNA segments from Saccharomyces cerevisiae and primary characterization of their structures.
Until recently it was assumed that any short segment of DNA could be approximated as a straight rod. Many instances, however, have been reported in which the helical axis is curved. We have devised a simple method for selective identification of DNA segments containing a sequence-directed bend (curvature), by means of a two-dimensional polyacrylamide gel electrophoresis. In order to gain general insights into the structural features and the functional significance of sequence-directed bends, a bank of plasmids carrying bent DNA inserts from the Saccharomyces cerevisiae total genomic DNA was constructed. Primary characterizations of a set of bent DNA segments randomly cloned from S. cerevisiae are presented. One of the cloned DNA segments appears to be derived from a yeast plasmid, the 2 microns circle DNA.
Mizuno
T
T
Laboratory of Microbiology, School of Agriculture, Nagoya University, Japan.
Itoh
K
K
D001483:Base Sequence; D003001:Cloning, Molecular; D004270:DNA, Circular / Q000235:genetics*; D004271:DNA, Fungal / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D004270:DNA, Circular; D004271:DNA, Fungal