Expression of Leu-M1 antigens in carcinoma of the urinary bladder.
Anti-Leu-M1 antibody, which is well known to react with a human myelomonocytic antigen, was found to react with bladder carcinoma, but never with the normal bladder epithelium. Eighty-six cases of transitional cell carcinoma of the bladder were tested in sections of formalin-fixed and paraffin-embedded tissues by using an avidin-biotin immunoperoxidase technique. Fifty per cent of these cases were positive in Leu-M1, while the other three monoclonal antibodies (HBA4, HBG9 and HBH8), which were produced against a human bladder carcinoma cell line KU-1, reacted in 17 per cent, 27 per cent and 64 per cent of these cases respectively. Reactivities of these antibodies to 33 cases of normal bladder epithelium were Leu-M1, 0 per cent; HBA4, 0 per cent; HBG9, 0 per cent and HBH8, 30 per cent, respectively. Expression of Leu-M1 antigens in bladder epithelium seems to be specific for malignant change and highly frequent in the carcinoma patients. The relation between Leu-M1 expression and a degree or stage in bladder carcinomas, however, was not significant in our studies.
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D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000945:Antigens, Differentiation, T-Lymphocyte; D000951:Antigens, Neoplasm / Q000032:analysis* / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology*; D002295:Carcinoma, Transitional Cell / Q000276:immunology* / Q000473:pathology; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D009364:Neoplasm Recurrence, Local; D009367:Neoplasm Staging; D001743:Urinary Bladder / Q000276:immunology; D001749:Urinary Bladder Neoplasms / Q000276:immunology* / Q000473:pathology
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D000951:Antigens, Neoplasm; D000954:Antigens, Surface