Cloning type-II restriction and modification genes.
We have cloned into Escherichia coli the genes for 38 type-II bacterial modification methyltransferases. The clones were isolated by selecting in vitro for protectively modified recombinants. Most of the clones modify their DNA fully but a substantial number modify only partially. In approximately one-half of the clones, the genes for the corresponding endonucleases are also present. Some of these clones restrict infecting phages and others do not. Clones carrying endonuclease genes but lacking methyltransferase genes have been found, in several instances, to be viable.
Lunnen
K D
KD
New England Biolabs, Inc., Beverly, MA 01915.
Barsomian
J M
JM
Camp
R R
RR
Card
C O
CO
Chen
S Z
SZ
Croft
R
R
Looney
M C
MC
Meda
M M
MM
Moran
L S
LS
Nwankwo
D O
DO
D001426:Bacterial Proteins / Q000235:genetics*; D003001:Cloning, Molecular / Q000379:methods*; D015254:DNA Modification Methylases / Q000235:genetics*; D004269:DNA, Bacterial / Q000378:metabolism; D004279:DNA, Viral / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D011994:Recombinant Proteins / Q000235:genetics*
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004279:DNA, Viral; D011994:Recombinant Proteins; D015254:DNA Modification Methylases; D015252:Deoxyribonucleases, Type II Site-Specific