Bradykinin stimulates increased intracellular calcium in papillary collecting tubules of the rabbit.
The effect of bradykinin on cytoplasmic Ca2+ concentration in rabbit papillary collecting tubule cells was determined using the fluorescent indicator Quin 2. Bradykinin stimulated a rapid increase in intracellular Ca2+. The rise in Ca2+ was dose dependent, persisted for less than 90 seconds and was independent of extracellular calcium. The ED50 for bradykinin induced changes in [Ca2+]i paralleled that observed previously for hormone-induced PGE2 formation as well as for inositol trisphosphate labelling. These studies provide additional support for the role of Ca2+ as a second messenger for bradykinin in renal papillary collecting tubule cells.
Shayman
J A
JA
Hruska
K A
KA
Morrison
A R
AR
D000634:Aminoquinolines / Q000378:metabolism; D000818:Animals; D001127:Arginine Vasopressin / Q000494:pharmacology; D001920:Bradykinin / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004533:Egtazic Acid / Q000494:pharmacology; D005453:Fluorescence; D007424:Intracellular Fluid / Q000378:metabolism; D007684:Kidney Tubules / Q000378:metabolism*; D007685:Kidney Tubules, Collecting / Q000187:drug effects / Q000378:metabolism*; D011817:Rabbits
D000634:Aminoquinolines; D001127:Arginine Vasopressin; D004533:Egtazic Acid; C035739:Quin2; D001920:Bradykinin; D002118:Calcium