[A modified medium, bile esculin-pyrrolidonyl-beta-naphthylamide, for rapid differentiation between enterococci and nonenterococci].
A new medium was developed by adding 40 mg pyrrolidonyl-beta-naphthylamide (PYR) to 1 l bile esculin (BE) agar, and was named as BE-PYR agar. To evaluate the efficacy of BE-PYR agar in differentiating enterococci from nonenterococci, we inoculated 207 strains of group D streptococci (including 157 enterococci and 50 nonenterococci) to BE-PYR agar. After incubating overnight at 35 degrees C incubator, following by applying the PYR reagent to test the color change of colonies, results indicate that the accuracy of BE-PYR agar in the differentiation of enterococci from nonenterococci reaches to 100%. Therefore, BE-PYR agar was considered to be an effective laboratory tool in further differentiation of group D streptococci.
Wu
S J
SJ
Department of Microbiology, National Yang-Ming Medical College, Taipei, R.O.C.
Chow
S F
SF
Li
S N
SN
Tsai
W C
WC
D000362:Agar; D015373:Bacterial Typing Techniques / Q000379:methods; D001646:Bile; D003374:Coumarins; D003470:Culture Media; D004929:Esculin; D005069:Evaluation Studies as Topic; D011760:Pyrrolidinones; D013291:Streptococcus / Q000145:classification*
D003374:Coumarins; D003470:Culture Media; D011760:Pyrrolidinones; D004929:Esculin; C034099:pyrrolidonyl-beta-naphthylamide; D000362:Agar