Effects of heat inactivation on HIV antibody screening and confirmatory test systems.
In order to evaluate the effect of heat inactivation on serum undergoing testing for HIV antibody, 100 heat-inactivated and nonheat-inactivated serum samples were tested by two modifications of Abbott's screening assays for human T-cell lymphotropic virus type-III (lot numbers 1037 and 3036) and by two confirmatory assays (Cambridge BioScience CBre3-EIA; Damon Corporation Western blot). The samples consisted of 75 HIV antibody-negative and 25 HIV antibody-positive sera. The specimens were divided into two equal aliquots. One set was not subjected to heat inactivation, while the others were subjected to heat inactivation at 56 degrees C for 30 min. Heat inactivation had no significant effect on the HIV-position sera; however, heat-inactivated, negative sera evaluated by Abbott lot numbers 1037 and 3036 resulted in false-positive rates of 8% and 7%, respectively. No false positives were generated by the two confirmatory assays; however, the CBre3-EIA recombinant envelope protein assay had a significantly increased optical density reading following heat inactivation of the negative sera. The Western blot procedure used in the study was not affected by heat inactivation.
Fipps
D R
DR
Department of Virus Disease, Walter Reed Army Institute of Research, Walter Reed Army Medical Center, Washington, DC 20307-5100.
Damato
J J
JJ
Burke
D S
DS
D015492:AIDS Serodiagnosis / Q000592:standards; D015153:Blotting, Western; D004355:Drug Stability; D005189:False Positive Reactions; D015483:HIV Antibodies / Q000032:analysis*; D006358:Hot Temperature; D006801:Humans; D007124:Immunoenzyme Techniques
D015483:HIV Antibodies