title: Effects of calcium on transendothelial albumin transfer and electrical resistance.
abstract: Nicolaysen, and more recently Kern and Malik, reported that chelation of calcium increased microvascular hydraulic conductivity and albumin permeability in isolated perfused lungs. To begin to understand how calcium affects endothelial function we examined the effect of calcium chelation on an in vitro endothelium. Chelation of calcium with ethyleneglycol-bis(beta-aminoethylether)-N,N'-tetraacetic acid increased the rate of transendothelial albumin transfer by 125%. Reincubation of the endothelium in calcium-repleted medium restored the rate of transfer to its original value. Chelation of extracellular calcium abolished transendothelial electrical resistance. The transendothelial electrical resistance was also restored to normal by reincubation of the endothelium in calcium-repleted medium. Chelation of extracellular calcium caused adjacent endothelial cells to retract from one another, and normal apposition of adjacent cells was restored after reincubation in calcium-repleted medium. Chelation of extracellular calcium produced a centripetal retraction of the peripheral band of actin in individual endothelial cells, and the actin band resumed its normal location after reincubation in calcium-repleted medium. Calcium is an important determinant of endothelial integrity and alterations in calcium produce dynamic changes in endothelial barrier properties and in endothelial-cell shape.
authors.forename: D M
authors.initials: DM
authors.lastname: Shasby
authors.forename: S S
authors.initials: SS
authors.lastname: Shasby
mesh_terms: D000418:Albumins / Q000378:metabolism* / Q000502:physiology; D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D002122:Calcium Chloride / Q000494:pharmacology; D002478:Cells, Cultured; D004533:Egtazic Acid / Q000494:pharmacology; D004553:Electric Conductivity; D004727:Endothelium / Q000166:cytology / Q000378:metabolism / Q000502:physiology; D008168:Lung / Q000166:cytology / Q000378:metabolism* / Q000502:physiology; D013552:Swine
chemical_list: D000418:Albumins; D004533:Egtazic Acid; D002122:Calcium Chloride; D002118:Calcium