Immunocytochemical staining of different cell types in vaginal smears by monoclonal anti-DNA methylase antibodies.
Enzymatic methylation of mammalian DNA is closely related to the replication process; also, the synthesis of DNA methylase, an enzyme that is responsible for this process, is cell cycle related. A monoclonal antibody against DNA methylase recognizes proliferatively active cells in the heterogeneous population. We used this antibody to identify the proliferative state of different cell types in normal vaginal smears and in smears of patients with precancerous lesions and cervical cancer. In all preparations, the normal epithelial cells remained unstained. The majority of cancer cells gave a positive immunocytochemical signal indicating the presence of DNA methylase antigen and the proliferative state. Staining was also observed in dyskaryotic cells, particularly in nuclei of parabasal-type cells.
Naujoks
H
H
Department of Clinical Cytology, Klinikum University, Frankfurt, Germany.
Siracky
J
J
Drahovsky
D
D
Pfeifer
G P
GP
D000911:Antibodies, Monoclonal; D015254:DNA Modification Methylases / Q000032:analysis* / Q000276:immunology; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D011230:Precancerous Conditions / Q000201:enzymology / Q000473:pathology*; D002583:Uterine Cervical Neoplasms / Q000201:enzymology / Q000473:pathology*; D014626:Vaginal Smears
D000911:Antibodies, Monoclonal; D015254:DNA Modification Methylases