title: Murine cells expressing an HLA molecule are specifically lysed by HLA-restricted antiviral human T cells.
abstract: Class I HLA (histocompatibility locus antigen) molecules are the targets of allospecific cytolytic T lymphocytes (CTL) in graft rejection, and constitute the restricting elements necessary for the interaction between antiviral CTL and virus-infected cells. Cells expressing only one HLA in the absence of other human molecules would provide a remarkable model for studying the function of these molecules. However, HLA+ murine cells transfected with human genes are generally not lysed by allospecific human CTL, and this is ascribed to insufficient HLA expression, lack of human beta 2-microglobulin, alteration of HLA molecules or absence of receptors for human T8 or LFA1 molecules in murine cells. Here we report, for the first time, the specific lysis of virus-infected HLA+ murine cells by HLA-restricted antiviral human CTL. Therefore, these murine cells constitute an excellent model for studying the role of HLA molecules.
authors.forename: E
authors.initials: E
authors.lastname: Gomard
authors.forename: B
authors.initials: B
authors.lastname: Begue
authors.forename: S
authors.initials: S
authors.lastname: Sodoyer
authors.forename: J L
authors.initials: JL
authors.lastname: Maryanski
authors.forename: B R
authors.initials: BR
authors.lastname: Jordan
authors.forename: J P
authors.initials: JP
authors.lastname: Levy
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface / Q000276:immunology; D003602:Cytotoxicity, Immunologic; D005818:Genetic Engineering; D006680:HLA Antigens / Q000276:immunology*; D006801:Humans; D009980:Influenza A virus / Q000276:immunology*; D051379:Mice; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface; D006680:HLA Antigens