[Immunosorption of apolipoproteins B. Optimization of the method in an in vitro bench test].
The aim of the present study was to determine optimum conditions for preparation and use of immunoadsorbents for removal of apolipoprotein B from plasma. An in vitro study was carried out to optimize different chromatography parameters: linear flow rate, apo B quantity loaded, gel regeneration conditions. Specificity of removal of apo B, stability of antibody covalent linkage and possible multiple use of immunoadsorbents were assessed. No significant loss of adsorption capacity was observed after sterilization and storage of immunoadsorbents.
Regnault
V
V
Unité INSERM U 284, Vandoeuvre-les-Nancy.
Rivat
C
C
Marcillier
P
P
Schooneman
F
F
Michaely
J P
JP
Stoltz
J F
JF
Duran Franzini
F
F
D001055:Apolipoproteins B / Q000097:blood*; D006801:Humans; D006937:Hypercholesterolemia / Q000097:blood; D007163:Immunosorbent Techniques / Q000295:instrumentation; D007164:Immunosorbents; D013045:Species Specificity; D013056:Spectrophotometry, Ultraviolet; D013242:Sterilization
D001055:Apolipoproteins B; D007164:Immunosorbents
Immunoépuration des apolipoprotéines B. Optimisation de la méthode sur banc d'essai in vitro.