title: [Mutagenicity of benzene metabolites by fluctuation test].
abstract: Mutagenicity of five benzene metabolites was examined by a fluctuation test modified by Gatehouse. The test was performed by using Salmonella typhimurium TA100 with or without a metabolic activation system. Catechol showed mutagenic activity with and without a metabolic activation system in the fluctuation test. Hydroxyhydroquinone, phenol, hydroquinone and p-benzoquinone were positive only with a metabolic activation system in this test. The Ames' plate method and preincubation method are widely used as a screening test for the mutagenic compounds. However, the fluctuation test is very useful in detecting mutagenic activity of chemicals which are negative in the Ames test though being a mutagen or carcinogen.
authors.affiliation: Department of Public Health, Jikei University School of Medicine.
authors.forename: N
authors.initials: N
authors.lastname: Koike
authors.forename: S
authors.initials: S
authors.lastname: Haga
authors.forename: N
authors.initials: N
authors.lastname: Ubukata
authors.forename: M
authors.initials: M
authors.lastname: Sakurai
authors.forename: H
authors.initials: H
authors.lastname: Shimizu
authors.forename: A
authors.initials: A
authors.lastname: Sato
mesh_terms: D000818:Animals; D001554:Benzene / Q000378:metabolism*; D016227:Benzoquinones; D001711:Biotransformation; D002396:Catechols / Q000633:toxicity*; D006873:Hydroquinones / Q000633:toxicity*; D008297:Male; D009152:Mutagenicity Tests / Q000379:methods; D019800:Phenol; D010636:Phenols / Q000633:toxicity*; D011809:Quinones / Q000633:toxicity*; D051381:Rats; D011919:Rats, Inbred Strains; D012486:Salmonella typhimurium / Q000235:genetics
chemical_list: D016227:Benzoquinones; D002396:Catechols; D006873:Hydroquinones; D010636:Phenols; D011809:Quinones; C013038:hydroxyhydroquinone; D019800:Phenol; C004532:quinone; D001554:Benzene; C034221:catechol; C031927:hydroquinone