[Construction of plasmids coding for diphtheria toxin fragments].
Plasmids coding different nontoxic derivatives (toxoids) of the diphtheria toxin were constructed. A secretion of toxoids that carry a signal sequence was found in the periplasmic space of E. coli and Erwinia carotovora. Toxoids without a signal sequence appear in the cytoplasm. We believe that the toxoids secreted in E. coli and E. carotovora cells undergo a limited proteolysis. According to the molecular weights of the fragments there are three targets for proteolysis. One of them being just between A- and B-fragments of the diphtheria toxin. The others are localised in the B-fragment. The role of E. coli signal peptidase in the specific cutting is discussed.
Zdanovskiĭ
A G
AG
Zdanovskaia
M V
MV
Zaĭtsev
E M
EM
Sviridov
V V
VV
Iakubovich
N V
NV
D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D004167:Diphtheria Toxin / Q000096:biosynthesis / Q000235:genetics*; D004885:Erwinia / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D006868:Hydrolysis; D010447:Peptide Hydrolases; D010957:Plasmids; D021382:Protein Sorting Signals / Q000096:biosynthesis / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics
D004167:Diphtheria Toxin; D021382:Protein Sorting Signals; D011994:Recombinant Proteins; D010447:Peptide Hydrolases
Konstruirovanie plazmid, kodiruiushchikh fragmenty difteriĭnogo toksina.