Partial purification of novel differentiation-inducing substances(s) from hot water extract of Japanese pine cone.
The effects of hot-water extract of pine cone (PCE) of Pinus parviflora Sieb. et Zucc. on the growth and differentiation of ML-1 cells, derived from a patient with human myeloblastic leukemia, were investigated. Growth of ML-1 cells was slightly inhibited at 3% (v/v) PCE, and a cytotoxic effect appeared at greater than 10%. Growth inhibition was accompanied by conversion to morphologically macrophage-like cells with alpha-naphthyl acetate esterase activity. In contrast, PCE dose-dependently increased the Fc receptor and nitroblue tetrazolium (NBT)-reducing activity up to 3%; above 3% its effect declined. Most of the cytotoxic activity was extracted from PCE with ethanol, and separated from the insoluble pellet, which contained the differentiation-inducing activity. The differentiation-inducing activity was eluted near the void volume on Sephadex G-200 gel filtration, with a 260-fold increase in the specific activity.
Sakagami
H
H
Takeda
K
K
Makino
Y
Y
Konno
K
K
D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification*; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D002850:Chromatography, Gel; D006358:Hot Temperature; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000473:pathology; D008970:Molecular Weight; D009580:Nitroblue Tetrazolium / Q000378:metabolism; D010936:Plant Extracts / Q000032:analysis*; D014197:Trees
D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts; D009580:Nitroblue Tetrazolium