[Pharmacological analysis of the effect of natural double-helical nucleic acids on the detoxifying function of the liver].
The effect of interferon inductors i.e. double stranded RNAs from S. cerevisiae and phage F6 on the liver detoxicating function was studied on noninbred albino mice. The liver detoxicating function was tested by duration of hexenal sleep. It was shown that intraperitoneal administration of the yeast and phage RNAs in doses of 1/5 LD50 for three times led to increasing of the narcotic sleep duration in the animals by 65 and 207 per cent, respectively. The effect was of the dose-dependent nature. The doses not inducing reliable inhibition of hexenal metabolism were equal to 1/10 LD50 for the yeast dsRNA and 1/27 LD50 for the phage dsRNA. The inhibitory effect of the dsRNAs was retained for 2-3 days after discontinuation of the drug use. When the dsRNAs were administered simultaneously with nembutal, an inductor of the liver microsomal enzymes, the dsRNAs eliminated its inducing effect. Simultaneous administration of alpha-tocopherol lowered the dsRNA effect on hexenal metabolism. The findings suggested that the dsRNA inhibitory effect on the liver detoxicating function was grounded on the mechanisms associated with inhibition of syntheses and activation of lipid peroxidation specific of the monooxygenase system under the action of the dsRNAs.
Masycheva
V I
VI
Morozova
E N
EN
Nadolinnaia
I G
IG
D000818:Animals; D004790:Enzyme Induction / Q000187:drug effects; D007369:Interferon Inducers; D008099:Liver / Q000187:drug effects*; D008111:Liver Function Tests; D051379:Mice; D012317:RNA Phages / Q000235:genetics; D012330:RNA, Double-Stranded / Q000494:pharmacology*; D012441:Saccharomyces cerevisiae / Q000235:genetics; D012890:Sleep / Q000187:drug effects; D014810:Vitamin E / Q000494:pharmacology
D007369:Interferon Inducers; D012330:RNA, Double-Stranded; D014810:Vitamin E
Farmakologicheskiĭ analiz vliianiia prirodnykh dvuspiral'nykh nukleinovykh kislot na detoksitsiruiushchuiu funktsiiu pecheni.