Unidirectional replication of plasmid R100.
We isolated a 284 base-pair BamHI fragment of plasmid R100 that supports initiation of replication of a plasmid regardless of the orientation of the fragment. Analysis of the specific radioactivity of restriction fragments from 32P-labeled replication intermediates synthesized in vitro shows that replication of the plasmid carrying the 284 base-pair fragment is unidirectional. The direction of replication depends on the orientation of the fragment present in the plasmid. The 5' ends of the leading-strand DNA formed in the early stage of replication were mapped to a region downstream from the 284 base-pair fragment in the direction of replication. The lagging-strand DNA products were also identified and their 3' ends mapped to unique sites within the 284 base-pair fragment causing unidirectional replication of R100.
Miyazaki
C
C
Institute of Applied Microbiology, University of Tokyo, Japan.
Kawai
Y
Y
Ohtsubo
H
H
Ohtsubo
E
E
D001345:Autoradiography; D001483:Base Sequence; D004261:DNA Replication; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics*; D004926:Escherichia coli; D008969:Molecular Sequence Data; D009154:Mutation; D011815:R Factors; D013997:Time Factors
D004269:DNA, Bacterial