title: Release of ethanolamine, but not of serine or choline, in rat pontine nuclei on stimulation of afferents from the cortex, in vivo.
abstract: Release of ethanolamine, serine, and choline in rat pontine nuclei on electrical stimulation of afferents from the cortex was investigated using in vivo push-pull cannula techniques. Ethanolamine was determined by using gas chromatographic techniques; serine was measured with a HPLC system; and choline was assayed with a luminescence method. Resting elution rates of ethanolamine, serine, and choline were 50.8 +/- 8.4, 34.8 +/- 12.6, and 1.16 +/- 0.20 pmol/5 min, respectively. Stimulation of the cortico-pontine tract evoked a highly significant 3.4-fold increase in release of ethanolamine, whereas serine and choline release was unaffected. Reactions in membrane phospholipids are most likely involved in the stimulation-dependent release of ethanolamine and special consideration was given to base-exchange reactions. Alternatively, a release from intracellular, possibly synaptic stores cannot be excluded.
authors.forename: H
authors.initials: H
authors.lastname: Perschak
authors.forename: M
authors.initials: M
authors.lastname: Wolfensberger
authors.forename: K Q
authors.initials: KQ
authors.lastname: Do
authors.forename: Y
authors.initials: Y
authors.lastname: Dunant
authors.forename: M
authors.initials: M
authors.lastname: Cuénod
mesh_terms: D000344:Afferent Pathways / Q000502:physiology*; D000818:Animals; D002540:Cerebral Cortex / Q000502:physiology*; D002794:Choline / Q000378:metabolism; D004558:Electric Stimulation; D019856:Ethanolamine; D004983:Ethanolamines / Q000378:metabolism*; D005260:Female; D007700:Kinetics; D008297:Male; D008636:Mesencephalon / Q000502:physiology; D011149:Pons / Q000502:physiology*; D051381:Rats; D011919:Rats, Inbred Strains; D012694:Serine / Q000378:metabolism
chemical_list: D004983:Ethanolamines; D012694:Serine; D019856:Ethanolamine; D002794:Choline