Expression of the staphylococcal protein A gene in Bacillus subtilis by gene fusions utilizing the promoter from a Bacillus amyloliquefaciens alpha-amylase gene.
Gene fusions of DNA sequences encoding protein A from Staphylococcus aureus (spa) with expression elements from an alpha-amylase gene from Bacillus amyloliquefaciens (amyEBamP) directed the synthesis and efficient secretion of protein A in Bacillus subtilis. The fusions were established on multicopy pUB110-based plasmid vectors, in contrast to the intact spa gene, which could not be stably established on plasmids in B. subtilis. Some of the resulting B. subtilis strains secreted protein A at levels in excess of 1 g/liter, demonstrating that a foreign protein encoded by an engineered gene can be secreted by B. subtilis at levels comparable to endogenous exoproteins.
Fahnestock
S R
SR
Fisher
K E
KE
D001407:Bacillus / Q000201:enzymology / Q000235:genetics; D001412:Bacillus subtilis / Q000235:genetics* / Q000378:metabolism; D004274:DNA, Recombinant; D005822:Genetic Vectors; D010957:Plasmids; D011401:Promoter Regions, Genetic; D013205:Staphylococcal Protein A / Q000096:biosynthesis / Q000235:genetics*; D013211:Staphylococcus aureus / Q000235:genetics* / Q000378:metabolism; D000516:alpha-Amylases / Q000235:genetics
D004274:DNA, Recombinant; D013205:Staphylococcal Protein A; D000516:alpha-Amylases