Differentiation of Plasmodium falciparum clones by means of a repetitive DNA probe.
A recombinant DNA probe, pBRK1-14, was constructed by inserting a repetitive DNA fragment of 753 base pairs obtained from Plasmodium falciparum, isolate K1, into the EcoRI/PstI cloning sites of pBR322. This probe could discriminate between different parasite clones derived from a single isolate by hybridization with Southern genomic blots.
Fucharoen
S
S
Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok, Thailand.
Tirawanchai
N
N
Wilairat
P
P
Panyim
S
S
Thaithong
S
S
D000818:Animals; D001345:Autoradiography; D003001:Cloning, Molecular; D015342:DNA Probes; D004274:DNA, Recombinant; D009693:Nucleic Acid Hybridization; D010963:Plasmodium falciparum / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid
D015342:DNA Probes; D004274:DNA, Recombinant