Synthesis and expression of a synthetic gene for the activated human c-Ha-ras protein.
A 576 base-pair DNA encoding human c-Ha-ras protein (p21) with a valine codon at position 12 has been synthesized by ligation of chemically synthesized oligodeoxyribonucleotides. The duplex was inserted into a plasmid with the E. coli tryptophan promoter and was expressed in E. coli efficiently.
Miura
K
K
Inoue
Y
Y
Nakamori
H
H
Iwai
S
S
Ohtsuka
E
E
Ikehara
M
M
Noguchi
S
S
Nishimura
S
S
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D013006:Growth Hormone / Q000235:genetics; D006801:Humans; D010957:Plasmids; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis*; D011519:Proto-Oncogenes
D011518:Proto-Oncogene Proteins; D013006:Growth Hormone