Underestimation of D-glucose phosphorylation as measured by 3H2O production from D-[2-3H]glucose.
In rat pancreatic islets, tumoral islet cells (RINm5F line), parotid gland, and in human erythrocytes, but not in rat hepatocytes, the production of 3H2O from D-[2-3H]glucose is 20-30% lower than from D-[5-3H]glucose. This coincides with the production of tritiated lactic acid from D-[2-3H]glucose and may be attributable to an intramolecular hydrogen transfer in the phosphoglucoisomerase reaction. It is concluded that the production of 3H2O from D-[2-3H]glucose is not a reliable tool to assess the total rate of hexose phosphorylation.
Malaisse
W J
WJ
Laboratory of Experimental Medicine, Brussels Free University, Belgium.
Yilmaz
M T
MT
Malaisse-Lagae
F
F
Sener
A
A
D000818:Animals; D002460:Cell Line; D004912:Erythrocytes / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D006019:Glycolysis; D006801:Humans; D066298:In Vitro Techniques; D007515:Islets of Langerhans / Q000378:metabolism*; D010190:Pancreatic Neoplasms; D010306:Parotid Gland / Q000378:metabolism*; D010766:Phosphorylation; D011865:Radioisotope Dilution Technique; D051381:Rats; D014316:Tritium
D014316:Tritium; D005947:Glucose