Comparison of broth enrichment and direct plating for the isolation of Campylobacter jejuni from dogs.
Two techniques for the isolation of Campylobacter jejuni from feces, direct plating and thioglycolate broth enrichment, were compared. A total of 272 rectal swab cultures were performed on 156 laboratory dogs. Campylobacter blood agar plates and Campylobacter thioglycolate broth were inoculated immediately upon sampling of the dogs. After incubation at 4 degrees C for 12 to 16 h, material from the Campylobacter thioglycolate medium was inoculated onto Campylobacter blood agar plates. A total of 157 samples were positive for C. jejuni; 154 were positive by the direct method and 112 were positive by the enrichment technique. Forty-five samples which were negative by the enrichment were positive by the direct method, and three samples which were negative by the direct method were positive for C. jejuni by the enrichment method. The use of the enrichment step resulted in an increase in the isolation rate from 56.6 to 57.7%.
Monfort
J D
JD
Department of Veterinary Preventive Medicine, College of Veterinary Medicine, Ohio State University, Columbus 43210.
Stills
H F
HF
Bech-Nielsen
S
S
D000818:Animals; D001431:Bacteriological Techniques; D002167:Campylobacter / Q000254:growth & development / Q000302:isolation & purification*; D003470:Culture Media; D003967:Diarrhea / Q000382:microbiology / Q000662:veterinary*; D004283:Dog Diseases / Q000382:microbiology*; D004285:Dogs; D005243:Feces / Q000382:microbiology
D003470:Culture Media