[Reversible suppression and activation of immunoglobulin genes in differentiated IgG+ sublines of human RPMI-6410t B-cells].
Differentiated human B-cells of the IgG+ sublines obtained as a result of switching from IgM to IgG synthesis in the 6410t line and its IgM+ lines gradually reduce the level of IgM secretion after the inductor removal. IgG synthesis can be partially or fully recovered by treating the IgG+ sublines with a polyclonal activator of B-lymphocytes, lipopolysaccharide W from Gram negative bacteria. In the conditions of certain regulatory effects, differentiated IgG+ cells are capable to pass reversibly in the state of functional rest and synthesis initiation.
Seregina
T M
TM
Mekshenkov
M I
MI
D001402:B-Lymphocytes / Q000166:cytology* / Q000187:drug effects / Q000276:immunology; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D002478:Cells, Cultured; D004926:Escherichia coli; D005784:Gene Amplification / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects; D005803:Genes, Immunoglobulin / Q000187:drug effects; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000235:genetics*; D008070:Lipopolysaccharides / Q000494:pharmacology; D012475:Salmonella; D013489:Suppression, Genetic / Q000187:drug effects; D014734:Vibrio cholerae
D007074:Immunoglobulin G; D008070:Lipopolysaccharides
Obratimaia supressiia i aktivatsiia genov immunoglobulinov v differentsirovannykh IgG+-subliniiakh B-kletok cheloveka RPMI-6410t.