Gene manipulation by homologous recombination in Escherichia coli.
The use of homologous recombination in Escherichia coli is described as a tool for DNA manipulation. The utility of the method is illustrated by the addition of 3'-flanking sequences to a dhfr minigene by plasmid-phage recombination involving a supF-containing dhfr minigene plasmid and a lambda Charon4A phage containing the 3' end of the dhfr gene. In addition, other uses of both plasmid-phage and phage-phage recombination in gene manipulation are described.
Crouse
G F
GF
Department of Biology, Emory University, Atlanta, GA 30322.
McEwan
R N
RN
D001435:Bacteriophages / Q000235:genetics; D002872:Chromosome Deletion; D003360:Cosmids; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D007438:Introns; D010957:Plasmids; D011995:Recombination, Genetic