title: Rapid hydrophobic grid membrane filter-enzyme-labeled antibody procedure for identification and enumeration of Escherichia coli O157 in foods.
abstract: An O-antigen-specific monoclonal antibody, labeled by horseradish peroxidase-protein A, was used in a hydrophobic grid membrane filter-enzyme-labeled antibody method for rapid detection of Escherichia coli O157 in foods. The method yielded presumptive identification within 24 h and recovered, on average, 95% of E. coli O157:H7 artificially inoculated into comminuted beef, veal, pork, chicken giblets, and chicken carcass washings. In food samples from two outbreaks involving E. coli O157:H7, the organism was isolated at levels of up to 10(3)/g. The lower limit of sensitivity was 10 E. coli O157 per g of meat. Specific typing for E. coli O157:H7 can be achieved through staining with labeled H7 antiserum or tube agglutination.
authors.affiliation: Bureau of Microbial Hazards, Health Protection Branch, Tunney's Pasture, Ottawa, Ontario, Canada.
authors.forename: E C
authors.initials: EC
authors.lastname: Todd
authors.forename: R A
authors.initials: RA
authors.lastname: Szabo
authors.forename: P
authors.initials: P
authors.lastname: Peterkin
authors.forename: A N
authors.initials: AN
authors.lastname: Sharpe
authors.forename: L
authors.initials: L
authors.lastname: Parrington
authors.forename: D
authors.initials: D
authors.lastname: Bundle
authors.forename: M A
authors.initials: MA
authors.lastname: Gidney
authors.forename: M B
authors.initials: MB
authors.lastname: Perry
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D015169:Colony Count, Microbial; D004926:Escherichia coli / Q000254:growth & development* / Q000276:immunology / Q000302:isolation & purification; D005260:Female; D005516:Food Microbiology; D006825:Hybridomas; D007124:Immunoenzyme Techniques; D008460:Meat; D051379:Mice
chemical_list: D000911:Antibodies, Monoclonal