The rotating 6-hydroxydopamine-lesioned mouse as a model for assessing functional effects of neuronal grafting.
Mice were first administered intrastriatal injections of 6-hydroxydopamine and subsequently a sub-group was given neural cell suspension grafts prepared from 14-day-old fetal ventral mesencephalic mouse tissue. Six and 8 weeks after transplantation the mice in the grafted group exhibited a significant reduction in amphetamine-induced turning behaviour towards the lesioned side compared to non-grafted lesioned controls. Six of the 7 mice that had surviving grafts containing histofluorescent dopamine neurons eventually showed a reversed motor side bias with more amphetamine-induced turning in a direction away from the transplant.
Brundin
P
P
Isacson
O
O
Gage
F H
FH
Prochiantz
A
A
Björklund
A
A
D000661:Amphetamine / Q000494:pharmacology*; D000818:Animals; D001480:Basal Ganglia Diseases / Q000139:chemically induced / Q000628:therapy; D003342:Corpus Striatum; D004195:Disease Models, Animal; D004298:Dopamine / Q000502:physiology*; D006892:Hydroxydopamines / Q000494:pharmacology*; D008636:Mesencephalon / Q000637:transplantation*; D051379:Mice; D009069:Movement Disorders / Q000139:chemically induced / Q000628:therapy*; D016627:Oxidopamine
D006892:Hydroxydopamines; D016627:Oxidopamine; D000661:Amphetamine; D004298:Dopamine