A cytotoxic monoclonal antibody produced from a human urinary bladder carcinoma cell line (TSGH-8301).
A mouse monoclonal antibody, designated 1G3.10, directed against a human urinary bladder cancer cell line TSGH-8301 was generated using the conventional hybridoma technique. It was of the IgG3 subclass with a restricted specificity to bladder cancer and several epithelial cancers, as detected on a panel of various cell lines and tissues by radioimmunoassay and indirect immunofluorescence methods. It also exhibited a strong binding activity to human group A red blood cells, as demonstrated in hemagglutination and absorption tests. Radioimmunoprecipitation and thin-layer chromatography revealed that the antigens recognized by the antibody are a mixture of glycopeptides (M.W. 30, 54, and 108 KD) and glycolipid (retardation factor 0.67-0.83). From a fluorescence activated cell sorter (FACS) study, direct killing of tumor cells by the antibody at a concentration of more than 10 micrograms/ml was observed. This antibody could also mediate both antibody-dependent cellular cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC) in vitro when tested in a 4-hr 51Cr-release test. These results suggest that the monoclonal antibody 1G3.10 may provide an agent for further immunodiagnosis and the treatment of human bladder cancer.
Ma
C P
CP
Department of Surgery, Tri-Service General Hospital, Taipei, Taiwan, R.O.C.
Yeh
M Y
MY
Yu
D S
DS
Jiang
S Y
SY
Wang
C C
CC
Chang
S Y
SY
Han
S H
SH
D000017:ABO Blood-Group System / Q000276:immunology; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D000920:Antibody-Dependent Cell Cytotoxicity; D000951:Antigens, Neoplasm / Q000276:immunology; D003165:Complement System Proteins / Q000276:immunology; D005434:Flow Cytometry; D006017:Glycolipids / Q000276:immunology; D006020:Glycopeptides / Q000276:immunology; D006386:Hemagglutination Tests; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D007163:Immunosorbent Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D014407:Tumor Cells, Cultured; D001749:Urinary Bladder Neoplasms / Q000276:immunology*
D000017:ABO Blood-Group System; D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D006017:Glycolipids; D006020:Glycopeptides; D007074:Immunoglobulin G; D003165:Complement System Proteins