An automated method for the quasi-continuous analysis of degradation and transfer products during the enzymatic hydrolysis of oligosaccharides.
A method is presented which allows for the automated quasi-continuous analysis of the degradation and transfer products developing during the enzymatic hydrolysis of oligosaccharides. A liquid chromatographic system is integrated into the bypass of a small batch reactor which makes it possible to take oligomer spectra without any manual sample processing being necessary. The time intervals between analyses are substantially reduced by making use of an overlapping analysis technique. Postcolumn derivatization with an orcinol sulfuric acid reagent gives a high sensitivity for carbohydrates. The great potential of this method is demonstrated for the characterization of a beta-glucosidase (pI 8.4) from Trichoderma reesei QM 9414 and an alpha 1,4-glucan glucohydrolase from Aspergillus niger with cellotetraose and maltohexaose as examplary substrates.
Schmid
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G
Wandrey
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C
D001234:Aspergillus niger / Q000201:enzymology; D002482:Cellulose / Q000031:analogs & derivatives*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005087:Glucan 1,4-alpha-Glucosidase / Q000378:metabolism; D006868:Hydrolysis; D009844:Oligosaccharides / Q000032:analysis* / Q000378:metabolism; D013780:Tetroses; D014242:Trichoderma / Q000201:enzymology; D001617:beta-Glucosidase / Q000378:metabolism
D009844:Oligosaccharides; D013780:Tetroses; C016549:maltohexaose; C048468:cellotetraose; D002482:Cellulose; D001617:beta-Glucosidase; D005087:Glucan 1,4-alpha-Glucosidase