An evaluation of 1-hexadecylpyridinium chloride as a decontaminant in the primary isolation of Mycobacterium bovis from bovine lesions.
For the primary isolation of Mycobacterium bovis from bovine lesions, 1-hexadecylpyridinium chloride (HPC) at a concentration of 0.75% was as effective as 2% NaOH in controlling the growth of contamination. The advantages of using HPC over NaOH are that it is a rapid one-step procedure not requiring neutralisation with acid, it is less toxic to M. bovis thus increasing isolation rates, and it promotes the earlier appearance of colonies.
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CSIRO, Division of Animal Health, Animal Health Research Laboratory, Parkville, Vic., Australia.
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D000818:Animals; D001431:Bacteriological Techniques; D002417:Cattle; D002594:Cetylpyridinium / Q000031:analogs & derivatives / Q000494:pharmacology / Q000592:standards*; D003666:Decontamination / Q000592:standards*; D009163:Mycobacterium bovis / Q000302:isolation & purification*; D011726:Pyridinium Compounds / Q000592:standards*; D012972:Sodium Hydroxide / Q000494:pharmacology / Q000592:standards*; D013997:Time Factors
D011726:Pyridinium Compounds; D012972:Sodium Hydroxide; D002594:Cetylpyridinium