Sterol composition of a delta 5,7-sterol-rich strain of Saccharomyces cerevisiae during batch growth.
Sterol composition was examined during batch growth on complex media containing ethanol, molasses or glucose as the carbon source. The molasses-grown cells exhibited a balanced sterol composition throughout growth, maintaining the proportion of ergosterol to 24:28-dehydroergosterol equal to 1.4. The negative effect of glucose on sterol synthesis manifested itself by decreasing the accumulation of 24:28-dehydroergosterol and total sterols but not of ergosterol. Using ethanol as the sole carbon source, a large amount of 24:28-dehydroergosterol accumulated, partly at the expense of other sterols. The gradual addition of nitrogen source during growth significantly decreased the accumulation of ergosterol, 24:28-dehydroergosterol and of total sterols. A general scheme of regulation of sterol synthesis in baker's yeast is presented.
Novotný
C
C
Institute of Microbiology, Czechoslovak Academy of Sciences, Prague.
Bĕhalová
B
B
Struzinský
R
R
Novák
M
M
Zajícek
J
J
D000641:Ammonia; D000693:Anaerobiosis; D002244:Carbon / Q000378:metabolism; D003470:Culture Media; D004875:Ergosterol / Q000031:analogs & derivatives* / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000378:metabolism; D000431:Ethanol; D005947:Glucose; D006903:Hydroxymethylglutaryl CoA Reductases / Q000378:metabolism; D008965:Molasses; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000254:growth & development* / Q000378:metabolism; D013261:Sterols / Q000096:biosynthesis / Q000378:metabolism
D003470:Culture Media; D013261:Sterols; C010905:dehydroergosterol; D000431:Ethanol; D002244:Carbon; D000641:Ammonia; D006903:Hydroxymethylglutaryl CoA Reductases; D005947:Glucose; D004875:Ergosterol