Microencapsulated hepatocytes for bioartificial liver support.
Free hepatocytes, harvested from normal rat livers by portal vein collagenase perfusion, were encapsulated within alginate-polylysine membranes and served as a liver support system. The encapsulated hepatocytes remained viable and were able to synthesize protein for up to 3 weeks in culture. Allografts of encapsulated hepatocytes replaced the function of a damaged liver and reduced the mortality rate among rats with galactosamine-induced fulminant hepatic failure.
Cai
Z H
ZH
University of Toronto, Department of Physiology, Ontario, Canada.
Shi
Z Q
ZQ
O'Shea
G M
GM
Sun
A M
AM
D000418:Albumins / Q000096:biosynthesis; D000818:Animals; D001187:Artificial Organs; D002470:Cell Survival; D056486:Chemical and Drug Induced Liver Injury; D004339:Drug Compounding; D004591:Electrophoresis, Polyacrylamide Gel; D005688:Galactosamine; D008099:Liver / Q000166:cytology* / Q000378:metabolism; D008107:Liver Diseases / Q000628:therapy*; D016031:Liver Transplantation; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D000418:Albumins; D005688:Galactosamine