title: Bradykinin stimulates increased intracellular calcium in papillary collecting tubules of the rabbit.
abstract: The effect of bradykinin on cytoplasmic Ca2+ concentration in rabbit papillary collecting tubule cells was determined using the fluorescent indicator Quin 2. Bradykinin stimulated a rapid increase in intracellular Ca2+. The rise in Ca2+ was dose dependent, persisted for less than 90 seconds and was independent of extracellular calcium. The ED50 for bradykinin induced changes in [Ca2+]i paralleled that observed previously for hormone-induced PGE2 formation as well as for inositol trisphosphate labelling. These studies provide additional support for the role of Ca2+ as a second messenger for bradykinin in renal papillary collecting tubule cells.
authors.forename: J A
authors.initials: JA
authors.lastname: Shayman
authors.forename: K A
authors.initials: KA
authors.lastname: Hruska
authors.forename: A R
authors.initials: AR
authors.lastname: Morrison
mesh_terms: D000634:Aminoquinolines / Q000378:metabolism; D000818:Animals; D001127:Arginine Vasopressin / Q000494:pharmacology; D001920:Bradykinin / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004533:Egtazic Acid / Q000494:pharmacology; D005453:Fluorescence; D007424:Intracellular Fluid / Q000378:metabolism; D007684:Kidney Tubules / Q000378:metabolism*; D007685:Kidney Tubules, Collecting / Q000187:drug effects / Q000378:metabolism*; D011817:Rabbits
chemical_list: D000634:Aminoquinolines; D001127:Arginine Vasopressin; D004533:Egtazic Acid; C035739:Quin2; D001920:Bradykinin; D002118:Calcium