title: Requirement of macrophages (monocytes) for the induction of interleukin 2 receptors on B lymphocytes.
abstract: The role of macrophages (monocytes) for the induction of interleukin 2 receptors (IL 2R) on human B lymphocytes was studied by a direct immunofluorescence method with the use of a fluoresceinated anti-IL 2R monoclonal antibody and a flow cytofluorometer. Highly purified B lymphocytes alone did not induce IL 2R on their surface by stimulation with Staphylococcus aureus Cowan I, anti-mu antibody, or pokeweed mitogen. However, the addition of monocytes successfully induced IL 2R on B lymphocytes stimulated with these mitogens in a dose-dependent manner. Interleukin 1 (IL 1) produced by monocytes could partially replace the accessory function of monocytes. In accordance with these results, the proliferation of B lymphocytes and the differentiation to immunoglobulin-producing cells in response to IL 2 were also dependent on monocytes or IL 1. These results suggest that the accessory function of macrophages for IL 2-induced B cell activation is primarily on the induction of IL 2R on B lymphocytes.
authors.forename: F
authors.initials: F
authors.lastname: Shirakawa
authors.forename: Y
authors.initials: Y
authors.lastname: Tanaka
authors.forename: S
authors.initials: S
authors.lastname: Eto
authors.forename: H
authors.initials: H
authors.lastname: Suzuki
authors.forename: U
authors.initials: U
authors.lastname: Yamashita
mesh_terms: D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D002454:Cell Differentiation; D006801:Humans; D007376:Interleukin-2 / Q000378:metabolism* / Q000502:physiology; D008213:Lymphocyte Activation; D008264:Macrophages / Q000276:immunology*; D009000:Monocytes / Q000276:immunology*; D011947:Receptors, Antigen, B-Cell / Q000096:biosynthesis; D011971:Receptors, Immunologic / Q000096:biosynthesis*; D015375:Receptors, Interleukin-2
chemical_list: D007376:Interleukin-2; D011947:Receptors, Antigen, B-Cell; D011971:Receptors, Immunologic; D015375:Receptors, Interleukin-2