Natural lectin activity in the haemolymph of Panstrongylus megistus (Heteroptera: Reduviidae).
The haemolymph of Panstrongylus megistus showed a natural lectin activity for a wide range of vertebrate erythrocytes. Agglutination was observed against all vertebrate erythrocytes tested (human ABO, duck, rabbit, mouse, sheep, chicken and cow). Cow erythrocytes showed the lowest titre. Concerning human erythrocytes, the lectin activity was similar in the types A+, B+ and AB+ while the highest activity was observed in the type O+. Determination of minimal inhibitory concentrations was carried out with human erythrocytes type O+. Agglutination was inhibited by several carbohydrates (rhamnose, D-galactose, raffinose, D-lactose and D-fucose). Rhamnose was reported as the strongest inhibitor (0.78 mM). The results suggest the presence of more than one lectin in the haemolymph of P. megistus.
Gomes
Y M
YM
Centro de Pesquisas Aggeu Magalhães-FIOCRUZ, Recife, PE, Brasil.
Furtado
A F
AF
De Carvalho
A B
AB
D000372:Agglutination Tests; D000818:Animals; D004912:Erythrocytes / Q000378:metabolism*; D006385:Hemagglutination Inhibition Tests; D006458:Hemolymph / Q000378:metabolism*; D006801:Humans; D037102:Lectins / Q000097:blood*; D010203:Panstrongylus / Q000378:metabolism*; D014225:Triatominae / Q000378:metabolism*
D037102:Lectins