Adult rat hepatocyte microcarrier culture. Comparison to the conventional dish culture system.
A technique has been devised to attach adult rat hepatocytes to collagen-coated dextran microcarriers. Cells were cultured serum-free for 2 d and their viability, enzyme activities, glucose metabolism, and hormone responsiveness were compared to data obtained from conventional dish cell culture. The two different culture methods showed no difference in cell viability and morphology. Microcarrier-cultured cells exhibited hormone responsiveness comparable to dish cultures; glycolysis could be activated three-fold by the sole addition of insulin, and gluconeogenesis was increased by 40 to 50% by glucagon. During the 48-h culture glucokinase and phosphoenolpyruvate carboxykinase activities declined at a similar rate in both culture systems. Long-term culture with 0.1 microM insulin prevented the decrease of glucokinase activity. Insulin responsiveness (activation of glycolysis) was still pronounced after 48 h in culture. The microcarrier technique establishes a new in vitro liver system in which acute and long-term hormonal actions can be investigated using the technical advantages of a suspension culture.
Athari
A
A
Institute für Biochemie, Universität Göttingen, FRG.
Unthan-Fechner
K
K
Schwartz
P
P
Probst
I
I
D000818:Animals; D002448:Cell Adhesion; D002470:Cell Survival; D002478:Cells, Cultured / Q000166:cytology*; D005943:Gluconeogenesis; D006019:Glycolysis / Q000187:drug effects; D007328:Insulin / Q000494:pharmacology; D008099:Liver / Q000166:cytology* / Q000201:enzymology / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D010100:Oxygen / Q000378:metabolism; D051381:Rats; D013997:Time Factors
D007328:Insulin; D010100:Oxygen