title: Activation of Ha-ras in human chronic granulocytic and chronic myelomonocytic leukaemia.
abstract: DNAs from chronic granulocytic leukaemia (CGL) and chronic myelomonocytic leukaemia (CMML) were assayed for transforming genes by transfection into NIH 3T3 cells. Foci DNA was tagged with a geneticin-resistance cosmid, then followed through a drug selection and tumorigenicity assay. Activated Ha-ras genes, with point mutations at codon 12 (glycine to valine) were subsequently detected. The mutation was detected in the original samples by either MspI/HpaII digestion or polymerase chain reaction (PCR). Although mutations in the ras gene family may occur frequently in leukaemias, these are the first examples of Ha-ras mutations in CGL (blast crisis) and CMML.
authors.affiliation: Institute of Cancer Research, London, U.K.
authors.forename: J
authors.initials: J
authors.lastname: Gow
authors.forename: D
authors.initials: D
authors.lastname: Hughes
authors.forename: C
authors.initials: C
authors.lastname: Farr
authors.forename: T
authors.initials: T
authors.lastname: Hamblin
authors.forename: D
authors.initials: D
authors.lastname: Oscier
authors.forename: R
authors.initials: R
authors.lastname: Brown
authors.forename: R A
authors.initials: RA
authors.lastname: Padua
mesh_terms: D000818:Animals; D001752:Blast Crisis / Q000235:genetics* / Q000378:metabolism; D002460:Cell Line; D003062:Codon; D004273:DNA, Neoplasm / Q000235:genetics; D005786:Gene Expression Regulation; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics* / Q000378:metabolism; D015477:Leukemia, Myelomonocytic, Chronic / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D009154:Mutation; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000235:genetics*; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis / Q000235:genetics*; D016283:Proto-Oncogene Proteins p21(ras); D014162:Transfection
chemical_list: D003062:Codon; D004273:DNA, Neoplasm; D009363:Neoplasm Proteins; D011518:Proto-Oncogene Proteins; C501009:HRAS protein, human; D016283:Proto-Oncogene Proteins p21(ras)