Three-dimensional reconstruction from serial section images by computer graphics.
A method is described which allows a three-dimensional object to be reconstructed from micrographs of serial sections using computer graphic techniques. The reconstructed object, which can be rotated three-dimensionally, is displayed on a color CRT and the surface of the object is shaded in order that it can be observed to provide an illusion of a three-dimensional structure. Using biological samples, some useful representation methods are demonstrated, which are a transparent display, smoothing of a reconstructed surface, cuts through a reconstructed model and a three-dimensional distribution representation of particles, and so forth. Furthermore, an example measuring the volume and surface area of reconstructed objects is shown.
Baba
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N
Department of Electrical Engineering, Kogakuin University, Tokyo, Japan.
Kanaya
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K
D000818:Animals; D003196:Computer Graphics; D004285:Dogs; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008854:Microscopy, Electron / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000648:ultrastructure; D014070:Tooth / Q000033:anatomy & histology