The coexistence of three neuroactive substances in amacrine cells of the chicken retina.
For the first time in the vertebrate retina, we have demonstrated that 3 neuroactive substances coexist in a single neuron. Using immunofluorescence, immunoperoxidase, and autoradiographic techniques, we found a subpopulation of amacrine cells in the chicken retina exhibited enkephalin- and neurotensin-like immunoreactivity and high-affinity uptake of glycine. Biochemical evidence showed that glycine release in this retina is inhibited by enkephalin and enhanced by neurotensin1.14. It is therefore possible that the two peptides form a push-pull system in self-regulating the glycine release from retinal amacrine cells.
Wu
S M
SM
Cullen Eye Institute, Baylor College of Medicine, Houston, TX 77030.
Lam
D M
DM
D000818:Animals; D001345:Autoradiography; D002645:Chickens; D004745:Enkephalins / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D005998:Glycine / Q000378:metabolism; D007124:Immunoenzyme Techniques; D009496:Neurotensin / Q000032:analysis*; D012160:Retina / Q000166:cytology*; D012165:Retinal Ganglion Cells / Q000166:cytology*; D014316:Tritium
D004745:Enkephalins; D014316:Tritium; D009496:Neurotensin; D005998:Glycine