Heterogeneity of 6'-N-acetyltransferases of type 4 conferring resistance to amikacin and related aminoglycosides in members of the family Enterobacteriaceae.
DNA-DNA hybridization and immunoblotting were used to assess the relatedness between the 6'-N-acetyltransferases of type 4 encoded by plasmid pAZ007 from a clinical isolate of Serratia marcescens and those encoded by NR79 and R5. The absence of detectable DNA-DNA homology and of immunological cross-reactivity suggests the existence of at least two distinct 6'-N-acetyltransferase type 4 genes that mediate amikacin resistance in gram-negative bacilli.
Tran Van Nhieu
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Laboratoire de Microbiologie Médicale, Université Paris VI, France.
Collatz
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D000123:Acetyltransferases / Q000235:genetics* / Q000276:immunology; D000583:Amikacin / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D015139:Blotting, Southern; D003429:Cross Reactions; D004269:DNA, Bacterial / Q000235:genetics; D015246:Deoxyribonuclease EcoRI; D004352:Drug Resistance, Microbial; D004755:Enterobacteriaceae / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology / Q000235:genetics; D015151:Immunoblotting; D007613:Kanamycin Resistance; D009693:Nucleic Acid Hybridization; D011815:R Factors; D012706:Serratia marcescens / Q000187:drug effects / Q000201:enzymology / Q000235:genetics
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D000583:Amikacin; D000123:Acetyltransferases; D015246:Deoxyribonuclease EcoRI