Role of plasma membrane and of cytomatrix in maintenance of intracellular to extracellular ion gradients in chicken erythrocytes.
Ultrastructural observations in combination with electron probe X-ray microanalysis on detergent (Brij 58) permeabilized (disruption of the plasma membrane) nucleated chicken erythrocytes support the view that a large fraction of cytoplasmic and nuclear K+ is not freely diffusible and that adsorption of K+ on detergent released mobilizable proteins exists within the cell. The data also suggest that the detergent proteins are normally immobilized by a detergent-resistant cytoskeleton so that they are not immediately free to diffuse from the cell for several minutes after detergent disruption of the plasma membrane.
Cameron
I L
IL
University of Texas Health Science Center, San Antonio 78284.
Hunter
K E
KE
Smith
N K
NK
Hazlewood
C F
CF
Ludany
A
A
Kellermayer
M
M
D000818:Animals; D002462:Cell Membrane / Q000502:physiology*; D002645:Chickens; D004577:Electron Probe Microanalysis; D004912:Erythrocytes / Q000166:cytology*; D005109:Extracellular Matrix / Q000502:physiology*; D008854:Microscopy, Electron; D008858:Microscopy, Phase-Contrast