Histidyl-tRNA synthetase of Chinese hamster ovary cells contains phosphoserine.
The Chinese hamster ovary cell line 2H2-5, which expresses elevated levels of histidyl-tRNA synthetase, was used to demonstrate that histidyl-tRNA synthetase contains phosphoserine. After growth of the cells in medium containing [32P]orthophosphate, histidyl-tRNA synthetase was isolated by partial purification and immunoprecipitation and shown to be a phosphoprotein. Phosphoamino acid analysis showed that histidyl-tRNA synthetase is phosphorylated on serine, as has previously been shown for threonyl-tRNA synthetase of CHO cells.
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D000604:Amino Acyl-tRNA Synthetases / Q000032:analysis*; D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D005260:Female; D006643:Histidine-tRNA Ligase / Q000032:analysis*; D010053:Ovary / Q000201:enzymology; D010750:Phosphoproteins / Q000032:analysis*; D010768:Phosphoserine / Q000032:analysis*; D012694:Serine / Q000031:analogs & derivatives*
D010750:Phosphoproteins; D010768:Phosphoserine; D012694:Serine; D000604:Amino Acyl-tRNA Synthetases; D006643:Histidine-tRNA Ligase