title: Microbial hydrolysis of organic nitriles and amides.
abstract: Nitrile-hydrating enzymes produced by bacteria and fungi catalyse the conversion of a large number of chemically diverse nitriles, including many economically important compounds used industrially for chemical synthesis of amides and acids. This paper presents data on two new, highly different nitrile-hydrolysing enzymes which were isolated in connection with our studies on enzymic nitrile transformations. Particular attention was paid to the enzymes' substrate specificities and sensitivity to substrate/product inhibition. One of our microbial isolates was a Rhodococcus sp. (strain CH5). This strain produces a constitutive hydratase that has a broad substrate spectrum, including aliphatic and aromatic nitriles, mononitriles and dinitriles, hydroxynitriles and amino-nitriles. It also produces a constitutive amidase of equally low substrate specificity. The hydratase/amidase system catalysed the hydrolysis of D,L-aminonitriles into racemic mixtures of amino acids. Strain CH5 is able to produce high concentrations of malonic acid monoamide from malononitrile and malonamide. The other isolate, Alcaligenes sp. (strain I4), can convert high concentrations of cyanoacetate into malonic acid, presumably by means of an aliphatic nitrilase that is specific for cyanoacetate. Enzyme kinetic experiments have shown that this enzyme is very resistant to both substrate and product inhibition.
authors.affiliation: Novo Industri A/S, Biochemical Synthesis Group, Bagsvaerd, Denmark.
authors.forename: K
authors.initials: K
authors.lastname: Ingvorsen
authors.forename: B
authors.initials: B
authors.lastname: Yde
authors.forename: S E
authors.initials: SE
authors.lastname: Godtfredsen
authors.forename: R T
authors.initials: RT
authors.lastname: Tsuchiya
mesh_terms: D000421:Alcaligenes / Q000378:metabolism; D000577:Amides / Q000378:metabolism*; D000581:Amidohydrolases / Q000378:metabolism; D000619:Aminohydrolases / Q000378:metabolism; D006836:Hydro-Lyases / Q000378:metabolism; D006868:Hydrolysis; D009570:Nitriles / Q000378:metabolism*; D012240:Rhodococcus / Q000378:metabolism
chemical_list: D000577:Amides; D009570:Nitriles; D000581:Amidohydrolases; C019665:amidase; D000619:Aminohydrolases; C021867:nitrilase; D006836:Hydro-Lyases; C048831:nitrile hydratase