Analysis of the dosage compensation of a specific transcript in Drosophila melanogaster.
The amount of steady-state level RNA complementary to the X-linked salivary gland secretion polypeptide gene Sgs-4 was measured in male and female third-instar larvae carrying one or two doses of a wild-type allele of the gene. RNA levels were found to be compensated in normal one-dose males and two-dose females and to be dosage-dependent within each sex. The presence of mutant alleles of male-less (mle) was found to reduce the level of Sgs-4 transcripts in males. These results support the contentions that dosage compensation is mediated by regulating the level of X-linked gene transcripts and that a product of the mle+ gene is involved in this process.
Breen
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Lucchesi
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JC
D000483:Alleles; D000818:Animals; D001483:Base Sequence; D004331:Drosophila melanogaster / Q000235:genetics*; D005260:Female; D005796:Genes; D007814:Larva; D008297:Male; D009693:Nucleic Acid Hybridization; D012469:Salivary Glands; D014158:Transcription, Genetic; D014960:X Chromosome