Molecular cloning, germ-line transformation, and transcriptional analysis of the zeste locus of Drosophila melanogaster.
Approximately 170 kilobase pairs (kb) of contiguous DNA sequences derived from bands 3A3,4 of the Drosophila melanogaster X chromosome have been isolated by molecular cloning. Sequences required for the wild-type expression of the zeste locus are located within a 6-kb fragment of this chromosomal region, as shown by phenotypic rescue of zeste mutants in P element-mediated germ-line transformation. Expression of zeste is correlated with a 2.2-kb poly(A)+ RNA species transcribed at all postzygotic stages of Drosophila development. Many zeste alleles, including several producing neomorphic phenotypes, are not associated with detectable rearrangements of DNA.
Gunaratne
P H
PH
Mansukhani
A
A
Lipari
S E
SE
Liou
H C
HC
Martindale
D W
DW
Goldberg
M L
ML
D000818:Animals; D001483:Base Sequence; D002869:Chromosome Aberrations; D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification; D004331:Drosophila melanogaster / Q000033:anatomy & histology / Q000235:genetics*; D005260:Female; D008297:Male; D010641:Phenotype; D014158:Transcription, Genetic; D014170:Transformation, Genetic; D014960:X Chromosome
D004247:DNA