Use of a monoclonal antibody in the diagnosis of infection by Dermatophilus congolensis.
A monoclonal antibody (McAb) to Dermatophilus congolensis was produced from murine hybridoma cultures and purified by affinity chromatography. Species specificity was demonstrated using indirect immunofluorescent staining; the McAb was shown to react with 10 D congolensis isolates but not with 10 Nocardia species isolates, a Rhodococcus and a Streptomyces species isolate. The McAb was used to demonstrate D congolensis in clinical material from confirmed bovine and ovine cases and presumptive equine cases of dermatophilosis by indirect immunofluorescent staining.
How
S J
SJ
Department of Veterinary Medicine and Animal Husbandry, Royal Veterinary College, North Mymms, Hertfordshire.
Lloyd
D H
DH
Lida
J
J
D000192:Actinomycetales / Q000276:immunology* / Q000302:isolation & purification; D000193:Actinomycetales Infections / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*; D000818:Animals; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial / Q000032:analysis; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis; D005455:Fluorescent Antibody Technique; D006734:Horse Diseases / Q000175:diagnosis; D006736:Horses; D009615:Nocardia / Q000276:immunology; D012240:Rhodococcus / Q000276:immunology; D012756:Sheep / Q000276:immunology / Q000382:microbiology; D012757:Sheep Diseases / Q000175:diagnosis; D013045:Species Specificity; D013302:Streptomyces / Q000276:immunology
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial