DNA fingerprinting of conjugative plasmids incompatible with R6K (IncX).
14 conjugative plasmids originated from different bacterial hosts and geographical sources were identified as members of the incompatibility group IncX with R6K as the reference plasmid. In spite of their close genetic relatedness (transfer and incompatibility properties), their molecular sizes ranged between 39 and 77 kb and DNA fingerprinting with endonucleases such as EcoRI, EcoRV, BamHI, BglI, BglII, PstI and SalI revealed a great variety of fragment patterns.
Prager
R
R
Institut für Experimentelle Epidemiologie, Wernigerode, GDR.
Tietze
E
E
Tschäpe
H
H
D003227:Conjugation, Genetic; D004269:DNA, Bacterial / Q000032:analysis*; D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli / Q000235:genetics*; D009710:Nucleotide Mapping; D010957:Plasmids; D015183:Restriction Mapping; D012706:Serratia marcescens / Q000235:genetics; D012760:Shigella / Q000235:genetics
D004269:DNA, Bacterial