In vitro effects of a mixture of Escherichia coli heat-stable enterotoxins on chloride flux in everted jejunal sacs of male pigs.
In vitro effects of a mixture of Escherichia coli heat-stable enterotoxins (STa and STb) on isolated jejunum of 3-week-old male pigs were studied, using everted intestinal sac techniques. Heat-stable enterotoxins increased chloride secretion and chloride absorption in everted intestinal sacs. The increase of secretory flux was greater than that for absorptive flux. Vasoactive intestinal peptide (6 x 10(-9) M) increased chloride secretion, but had no effect on chloride absorption. Neither vasoactive intestinal peptide nor pilocarpine (10(-5) M) had additive effect to ST. Secretory effects of ST were not blocked by atropine (2 x 10(-5) M), clonidine (10(-6) M), or morphine (4.2 x 10(-6) M).
Panichkriangkrai
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Department of Veterinary Physiology and Pharmacology, Iowa State University, Ames.
Ahrens
F A
FA
D000818:Animals; D001285:Atropine / Q000494:pharmacology; D001427:Bacterial Toxins / Q000494:pharmacology*; D002712:Chlorides / Q000378:metabolism*; D003000:Clonidine / Q000494:pharmacology; D004768:Enterotoxins / Q000494:pharmacology*; D004926:Escherichia coli; D029968:Escherichia coli Proteins; D007408:Intestinal Absorption; D007583:Jejunum / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D009020:Morphine / Q000494:pharmacology; D010862:Pilocarpine / Q000494:pharmacology; D013552:Swine / Q000378:metabolism*; D014660:Vasoactive Intestinal Peptide / Q000494:pharmacology
D001427:Bacterial Toxins; D002712:Chlorides; D004768:Enterotoxins; D029968:Escherichia coli Proteins; C034151:heat stable toxin (E coli); D010862:Pilocarpine; D014660:Vasoactive Intestinal Peptide; D009020:Morphine; D001285:Atropine; D003000:Clonidine