title: Interactions between Escherichia coli arginyl-tRNA synthetase and its substrates.
abstract: Interactions between Escherichia coli arginyl-tRNA synthetase and its substrates were extensively studied and distinctly demonstrated. Various approaches such as equilibrium dialysis, fluorescence titration, and substrate protection against heat inactivation of the enzyme were used for these studies. In the absence of other substrates, the equilibrium dissociation constants for arginine, ATP, and the cognate tRNA were about 70 microM, 0.85 mM, and 0.45 microM, respectively, at pH 7.5, in Tris buffer. The binding of arginine to the enzyme was affected neither by the presence of tRNA nor by the presence of ATP but was considerably enhanced when ATP and tRNA were both present at saturating concentrations. The dissociation constant in this case (about 16 microM) was very close to the Km (12 microM) for arginine during aminoacylation. The binding of ATP (the equilibrium dissociation constant KD approximately 0.85 mM) was not affected by the presence of arginine but was depressed in the presence of tRNA (KD became 3 mM). Arginyl-tRNA showed a dissociation constant of (4-5) X 10(-7) M which was not affected by the presence of a single other substrate. Possible explanations for the high Km for tRNA in the aminoacylation are discussed. Our results indicated pronounced interactions between substrates mediated by the enzyme under catalytic conditions. Periodate oxidation did not alter the tRNA binding to the enzyme. The oxidized tRNA still afforded protection against heat inactivation of the enzyme.
authors.affiliation: Shanghai Institute of Biochemistry, Academia Sinica, China.
authors.forename: S X
authors.initials: SX
authors.lastname: Lin
authors.forename: Q
authors.initials: Q
authors.lastname: Wang
authors.forename: Y L
authors.initials: YL
authors.lastname: Wang
mesh_terms: D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism*; D001126:Arginine-tRNA Ligase / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology*; D007700:Kinetics; D008433:Mathematics; D008962:Models, Theoretical; D011485:Protein Binding; D013379:Substrate Specificity
chemical_list: D000604:Amino Acyl-tRNA Synthetases; D001126:Arginine-tRNA Ligase