Erythropoietin gene expression in vitro and in vivo detected by in situ hybridization.
Macrophages derived from unstimulated and unseparated mouse bone marrow cells have been shown to release erythropoietin into the extracellular fluid. Additional proof that macrophages can produce the hormone would be a demonstration that the gene is expressed and the mature protein released. In situ hybridization using a 1.2 kb biotinylated erythropoietin DNA probe demonstrates that both cultured macrophages and those present in normal mouse bone marrow express the gene. These results are discussed in terms of the role played by the macrophage in the hemopoietic cellular microenvironment and indicate that a subpopulation is responsible for this function and that cell interactions play an important role in hemopoietic differentiation.
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Department of Transfusion Medicine, University of Ulm, German Red Cross Blood Bank, Oberer Eselsberg, Federal Republic of Germany.
Vogt
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Pentz
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D000818:Animals; D002478:Cells, Cultured; D004247:DNA / Q000235:genetics; D004921:Erythropoietin / Q000096:biosynthesis / Q000235:genetics*; D005455:Fluorescent Antibody Technique; D005796:Genes; D006412:Hematopoietic Stem Cells / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D009693:Nucleic Acid Hybridization
D004921:Erythropoietin; D004247:DNA