title: Biochemical evidence for presence of dopamine beta-hydroxylase in rat retina.
abstract: Previously, we demonstrated immunocytochemically that certain phenylethanolamine N-methyltransferase neurons in the inner nuclear layer of rat retina contain other catecholamine synthesizing enzymes, including tyrosine hydroxylase and aromatic-L-amino acid decarboxylase but not dopamine beta-hydroxylase (DBH), the norepinephrine biosynthetic enzyme. In the present study by using a sensitive radioenzymatic assay for DBH we demonstrated the presence of DBH enzymatic activity in retinal extracts. Immunocytochemical studies, however, failed to demonstrate DBH-immunoreactive perikarya even in animals pretreated with colchicine, an inhibitor of axonal transport. Probably causes for these discrepant findings are discussed.
authors.affiliation: Laboratory of Molecular Neurobiology, Cornell University Medical College, Burke Rehabilitation Center, White Plains, NY 10605.
authors.forename: D H
authors.initials: DH
authors.lastname: Park
authors.forename: T H
authors.initials: TH
authors.lastname: Joh
authors.forename: M
authors.initials: M
authors.lastname: Anwar
authors.forename: D A
authors.initials: DA
authors.lastname: Ruggiero
mesh_terms: D000818:Animals; D004299:Dopamine beta-Hydroxylase / Q000378:metabolism*; D007106:Immune Sera; D007124:Immunoenzyme Techniques; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values; D012160:Retina / Q000201:enzymology*; D012165:Retinal Ganglion Cells / Q000201:enzymology
chemical_list: D007106:Immune Sera; D004299:Dopamine beta-Hydroxylase