Reactions of the intracellular NADpool in the yeast S. cerevisiae after UV-C- or X-ray irradiation.
The reaction of the intracellular NADpool after irradiation of cells either with UV-C light or with X-rays was studied in four different strains of the yeast S. cerevisiae. We found neither in wildtype strains nor in radiation sensitive mutants remarkable changes in the NADpool within 2 h after irradiation. Preculture of cells in medium enriched with nicotinic acid, a precursor of NAD, influenced the intracellular NAD concentration only to a small extend in all strains, but enhanced the radiation resistance against UV-C significantly in one rad6 mutant strain. The uptake of NAD and NAC by all strains before and after irradiation with UV-C and X-ray was tested also. NAD generally is taken up by the cells to a very low extent before and after irradiation without irradiation-dose dependency. NAC is taken up by all strains before and after irradiation. Only the rad6 mutant exhibited an irradiation-dose dependent NAC-uptake after UV-C irradiation.
Winckler
K
K
Institut für Biophysik, Freie Universität Berlin, Germany.
Herfurth
E
E
D007700:Kinetics; D009243:NAD / Q000378:metabolism* / Q000528:radiation effects; D012441:Saccharomyces cerevisiae / Q000378:metabolism / Q000528:radiation effects*; D013045:Species Specificity; D014466:Ultraviolet Rays; D014965:X-Rays
D009243:NAD