Storage of human red blood cells and platelets. Some aspects concerning the factors leading to storage lesion characterized as morphological changes and vesiculation. Minireview based on a doctoral thesis.
1. Storage renders erythrocytes more responsive to thermally induced morphological changes, especially the shedding of microvesicles. 4-8 week old cells can be morphologically "rejuvenated" by heating. 2. If pH increases during storage of platelets an extensive loss of small particles occurs. The platelet disintegration is associated with a loss in the metabolic activity, discharge of LDH, increased susceptibility to phospholipid hydrolysis by phospholipase C and is found to be initiated during the actual preparation of platelet concentrates. 3. Activation of platelets during preparation can be decreased by shortening the first centrifugation time or by using adenine in the anticoagulant. 4. A 4 hour prestorage of the whole blood unit prior to centrifugation strongly decreases the activation of platelets upon stimuli and results in platelet concentrates much more stable to storage.
Solberg
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Department of Biochemistry, University of Tromsø, Norway.
D001792:Blood Platelets / Q000473:pathology*; D001793:Blood Preservation / Q000295:instrumentation / Q000379:methods*; D004912:Erythrocytes / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D013997:Time Factors