[Experimental microbiological criteria for the banking of full-thickness skin].
Experimental studies were conducted into the biological durability of homologous full skin grafts. Colony counts for that purpose were determined in deep-frozen specimens in weekly intervals. Specimens were stored at various temperatures (+4 degrees C, -20 degrees C, -70 degrees C, -80 degrees C), with and without preceding quick freezing. Storage at -70 degrees C with protracted freezing gradient of 1 degree C/minute proved to the most favourable variant with due consideration of microbiological criteria. The physiological resident flora of skin grafts after thawing was almost unchanged under these conditions.
Bäumer
F
F
Chirurgischen Klinik und Poliklinik, Universität Würzburg.
Hartmann
A A
AA
Henrich
H A
HA
D000818:Animals; D001431:Bacteriological Techniques; D003470:Culture Media; D004203:Disinfection / Q000379:methods; D012867:Skin / Q000382:microbiology; D013524:Surgical Flaps; D013552:Swine; D013556:Swine, Miniature; D014015:Tissue Banks
D003470:Culture Media
Mikrobiologisch-experimentelle Kriterien zum Banking von Vollhaut.