title: Phenylalanyl-tRNA synthetase from chloroplasts of a higher plant (Phaseolus vulgaris). Purification and comparison of its structural, functional, and immunological properties with those of the enzymes from the corresponding cytoplasm, the cyanobacterium Anacystis nidulans, and the photosynthetic green sulfur bacterium Chlorobium limicola.
abstract: Chloroplastic phenylalanyl-tRNA synthetase from bean leaves is purified under optimal protective conditions over 4,900-fold. Its apparent molecular weight is 78,000, as determined by gel filtration, with a dimeric subunit structure of alpha beta (alpha = 33,000 and beta = 42,000), as determined by sodium dodecyl sulfate gel electrophoresis. This indicates a drastic size reduction of 40% for each subunit compared to the corresponding cytoplasmic enzyme and a unique quaternary structure. Heterologous aminoacylation and substrate properties of ATP analogs indicate substantial differences in the topographies of the substrate binding domains of these two heterotopic intracellular plant enzymes. No common antigenic determinants with the bean cytoplasmic enzyme were detected by polyclonal antibodies against the chloroplastic enzyme. The same negative result applies to the immunological comparison with the partially purified enzymes from the cyanobacterium Anacystis nidulans and the photosynthetic green sulfur bacterium Chlorobium limicola that both have a molecular weight of 260,000.
authors.forename: R
authors.initials: R
authors.lastname: Rauhut
authors.forename: H J
authors.initials: HJ
authors.lastname: Gabius
authors.forename: F
authors.initials: F
authors.lastname: Cramer
mesh_terms: D000604:Amino Acyl-tRNA Synthetases / Q000302:isolation & purification*; D002736:Chloroplasts / Q000201:enzymology*; D000458:Cyanobacteria; D007887:Fabaceae / Q000201:enzymology*; D046911:Macromolecular Substances; D008970:Molecular Weight; D010652:Phenylalanine-tRNA Ligase / Q000302:isolation & purification*; D010946:Plants, Medicinal; D013329:Structure-Activity Relationship
chemical_list: D046911:Macromolecular Substances; D000604:Amino Acyl-tRNA Synthetases; D010652:Phenylalanine-tRNA Ligase