Flagellin parts acquiring a regular structure during polymerization are disposed on the molecule ends.
Flagellins of two Escherichia coli strains have been investigated by limited proteolysis and scanning microcalorimetry. It has been shown that a monomer flagellin consists of two parts: a central one cooperatively melting, rather resistant to proteases, and the other without a stable tertiary structure and thus easily degrading terminals. Just these terminals acquire a regular structure during polymerization. Core fragments of the two strains have been isolated and characterized.
Kostyukova
A S
AS
Institute of Protein Research, Academy of Sciences of the USSR, Moscow Region.
Pyatibratov
M G
MG
Filimonov
V V
VV
Fedorov
O V
OV
D001426:Bacterial Proteins / Q000378:metabolism; D002918:Chymotrypsin; D003488:Cyanogen Bromide; D004926:Escherichia coli / Q000378:metabolism*; D005408:Flagellin / Q000378:metabolism; D046911:Macromolecular Substances; D008970:Molecular Weight; D010446:Peptide Fragments / Q000032:analysis; D013816:Thermodynamics; D013820:Thermolysin; D014357:Trypsin
D001426:Bacterial Proteins; D046911:Macromolecular Substances; D010446:Peptide Fragments; D005408:Flagellin; D002918:Chymotrypsin; D014357:Trypsin; D013820:Thermolysin; D003488:Cyanogen Bromide