[Production of thermolabile enterotoxins of Escherichia coli by Yersinia enterocolitica and Yersinia pseudotuberculosis].
The plasmids pCG86 and RP4elt coding for thermolabile enterotoxins of Escherichia coli (LT) were transferred in conjugation to Yersinia enterocolitica and Yersinia pseudotuberculosis cells. Both plasmids were stably inherited by the recipient cells. The elt genes of the toxins were expressed in Yersinia cells at the level comparable to the one registered in Escherichia coli cells. In the broth cultures of transconjugant cells the major part of LT toxin is bound with cells (74-97%). The obtained data may serve an experimental basis in favour of possibility of Ent+ strains of Yersinia enterocolitica and Yersinia pseudotuberculosis formation in nature and expediency of search and diagnosing of such strains.
Titenko
B M
BM
Emdina
I A
IA
Lebedeva
S A
SA
Burlakova
O S
OS
Roshchina
N V
NV
D001427:Bacterial Toxins / Q000096:biosynthesis*; D003227:Conjugation, Genetic; D004768:Enterotoxins / Q000096:biosynthesis*; D004926:Escherichia coli; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial; D010957:Plasmids; D015008:Yersinia enterocolitica / Q000235:genetics / Q000378:metabolism*; D015011:Yersinia pseudotuberculosis / Q000235:genetics / Q000378:metabolism*
D001427:Bacterial Toxins; D004768:Enterotoxins; D029968:Escherichia coli Proteins; C027538:heat-labile enterotoxin, E coli
Obrazovanie termolabil'nykh enterotoksinov kishechnoĭ palochki vozbuditeliami kishechnogo ĭersinioza i psevdotuberkuleza.