title: Expression of the human apolipoprotein AI gene fused to the E. coli gene for beta-galactosidase.
abstract: The human apoAI gene was expressed in E. coli by in-frame fusion to a modified beta-galactosidase gene present in plasmid pUR291. The fused beta-galactosidase-apoAI gene product was expressed at a high level and was recognized by an anti-human apoAI antiserum. Besides the fused protein, at least one degradation product having an Mr similar to that of beta-galactosidase was present in high amounts in bacterial extracts. These results and those of a pulse-chase experiment indicate that degradation took place only in the apoAI moiety of the chimeric protein.
authors.forename: R
authors.initials: R
authors.lastname: Lorenzetti
authors.forename: A
authors.initials: A
authors.lastname: Sidoli
authors.forename: R
authors.initials: R
authors.lastname: Palomba
authors.forename: L
authors.initials: L
authors.lastname: Monaco
authors.forename: D
authors.initials: D
authors.lastname: Martineau
authors.forename: D A
authors.initials: DA
authors.lastname: Lappi
authors.forename: M
authors.initials: M
authors.lastname: Soria
mesh_terms: D016632:Apolipoprotein A-I; D001054:Apolipoproteins A / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004274:DNA, Recombinant / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics*; D005696:Galactosidases / Q000235:genetics*; D005798:Genes, Bacterial; D005809:Genes, Regulator; D006801:Humans; D008075:Lipoproteins, HDL / Q000235:genetics*; D008099:Liver / Q000378:metabolism; D010957:Plasmids; D001616:beta-Galactosidase / Q000235:genetics*
chemical_list: D016632:Apolipoprotein A-I; D001054:Apolipoproteins A; D004274:DNA, Recombinant; D008075:Lipoproteins, HDL; D005696:Galactosidases; D001616:beta-Galactosidase