[Genetic characteristics of repair-deficient cells from patients with homocystinuria].
DNA repair activity in cells after gamma-irradiation or treatment with 4-nitroguinoline-1-oxide was studied by alkaline elution of cells lysed on membrane filters, as well as by reactivation and induced mutagenesis of vaccinia virus in lymphocytes and fibroblasts obtained in skin biopsy from patients with homocystinuria. Disorders of the repair were demonstrated in lymphocytes by using both criteria. In all fibroblast passages repair disorder was only found for gamma-irradiation, the repair activity being comparable with that of lymphocytes. The level of spontaneous and gamma-induced mutations in vaccinia virus propagated in fibroblasts was increased at later culture passages.
Sinel'shchikova
T A
TA
L'vova
G N
GN
Shoniia
N N
NN
Zasukhina
G D
GD
D015112:4-Nitroquinoline-1-oxide; D002478:Cells, Cultured; D004260:DNA Repair / Q000187:drug effects / Q000528:radiation effects; D004277:DNA, Single-Stranded / Q000032:analysis; D005347:Fibroblasts / Q000378:metabolism / Q000382:microbiology; D005720:Gamma Rays; D006712:Homocystinuria / Q000235:genetics*; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism / Q000382:microbiology; D014616:Vaccinia virus / Q000254:growth & development; D014775:Virus Activation / Q000187:drug effects / Q000528:radiation effects
D004277:DNA, Single-Stranded; D015112:4-Nitroquinoline-1-oxide
Geneticheskaia kharakteristika kletok bol'nykh gomotsistinurieĭ s narushennoĭ sistemoĭ reparatsii DNK.