Purification and partial characterization of a bioactive substance from rat's vessel wall independent of prostacyclin production.
The bioactive substance from rat's vessel wall was purified by Sephadex G-75 gel filtration and by a combination of DEAE Cellulose ion exchange and Sephadex G-50 gel filtration chromatographies. Purifications of 12.5 fold and 70 fold over the initial material were achieved. PAGE of the purified material resulted in a single major band with a molecular weight estimated at 55kd-65kd. Trypsin (0.3 mg/ml) and chymotrypsin (30 mg/ml) abolished platelet antiaggregating activity. Neuraminidase (1.2 units) had no effect on platelet antiaggregating activity. This is the report of purification of aortic vessel wall antiaggregating activity independent of prostacyclin production.
Kempfer
A C
AC
Department of Hemostasis and Thrombosis, Academia Nacional de Medicina, Buenos Aires, Argentina.
Maugeri
N
N
Farias
C
C
Bermejo
E
E
Gimeno
M
M
Frontroth
J P
JP
Lazzari
M
M
D000818:Animals; D001011:Aorta; D002918:Chymotrypsin; D004355:Drug Stability; D004591:Electrophoresis, Polyacrylamide Gel; D011464:Epoprostenol / Q000096:biosynthesis*; D006801:Humans; D008970:Molecular Weight; D009131:Muscle, Smooth, Vascular / Q000378:metabolism*; D010974:Platelet Aggregation / Q000187:drug effects; D010975:Platelet Aggregation Inhibitors / Q000302:isolation & purification* / Q000502:physiology; D051381:Rats; D014357:Trypsin
D010975:Platelet Aggregation Inhibitors; D011464:Epoprostenol; D002918:Chymotrypsin; D014357:Trypsin