Regulatory region of the Aspergillus nidulans argB gene.
We have constructed a series of deletion plasmids which contain the Aspergillus nidulans argB gene for ornithine carbamoyltransferase (OTC). These deletions comprise the 5' upstream sequence of the argB gene. The pro- arg- strain of A. nidulans was transformed with the above plasmids. Several arg+ transformants of integration types I and II, obtained using each of the deletion plasmids, were studied, and their ability to de-repress OTC level by proline starvation was compared. It was concluded that nucleotides located between -150 and -50 bp upstream of the argB gene are significant for its cross-pathway regulation. This regulatory region contains three copies of the TGACTC hexanucleotide which is a cis-acting regulatory sequence of general amino acid control in yeast.
Goc
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Department of Plant Cytology and Genetics, Copernicus University, Toruń, Poland.
Wegleński
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P
D001233:Aspergillus nidulans / Q000201:enzymology / Q000235:genetics*; D001483:Base Sequence; D004271:DNA, Fungal; D004926:Escherichia coli / Q000235:genetics; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D009954:Ornithine Carbamoyltransferase / Q000235:genetics* / Q000378:metabolism; D010957:Plasmids; D011392:Proline / Q000378:metabolism; D012045:Regulatory Sequences, Nucleic Acid; D015183:Restriction Mapping; D014170:Transformation, Genetic
D004271:DNA, Fungal; D011392:Proline; D009954:Ornithine Carbamoyltransferase