Multicopy expression vector based on temperature-regulated lac repressor: expression of human immunodeficiency virus env gene in Escherichia coli.
A new expression vector (pBB1) has been constructed for the regulated expression of genes in Escherichia coli. Based on the pUC plasmids, the pBB1 carries lacIts allele of the lac repressor gene. This makes it possible to control expression of cloned genes by shifting the temperature from 30 degrees C to 42 degrees C. Thus the vector combines advantages of the pUC plasmids with convenient regulation by temperature. Expression of a fragment of HIV-1 env gene was achieved with the help of this vector and shown by enzyme-linked immunosorbent assay and Western-blot analysis.
Bukrinsky
M I
MI
D.I. Ivanovski Institute of Virology, Academy of Medical Sciences of the USSR, Moscow.
Barsov
E V
EV
Shilov
A A
AA
D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000235:genetics*; D005786:Gene Expression Regulation; D005822:Genetic Vectors; D015488:HIV Antigens / Q000096:biosynthesis / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics; D012097:Repressor Proteins / Q000235:genetics*; D013696:Temperature; D014157:Transcription Factors / Q000235:genetics*; D014759:Viral Envelope Proteins / Q000096:biosynthesis / Q000235:genetics
D015488:HIV Antigens; D011993:Recombinant Fusion Proteins; D012097:Repressor Proteins; D014157:Transcription Factors; D014759:Viral Envelope Proteins