title: Evolution of sister-chromatid exchanges (SCE) in rat bone marrow cells as a function of time after 2 Gy of whole-body neutron irradiation.
abstract: We scored sister-chromatid exchanges (SCE) in bone marrow cells in 3-month-old rats as a function of time after 2 Gy of whole-body neutron irradiation. This dose reduced the mean survival time to 445 days after irradiation, and induced more than one tumor per animal; by 200 days post irradiation, all animals bore tumors at autopsy, but bone marrow was not a significant target for tumor induction. In controls, the mean SCE/cell remained constant from 3 to 24 months of age (2.38 SCE/cell, S.D. = 0.21). Irradiation induced 2 distinct increases in SCE: the first occurred during the days following exposure, and the second, from days 150 to 240. Thereafter, SCE values formed a plateau at 3.37 SCE/cell (S.D. = 0.39) until day 650. Between the two increases (i.e. from days 15 to 150), SCE dropped to control values. Analysis of SCE distribution per cell shows that the entire dividing cell population altered homogeneously during the increase in SCE. These results suggest that in our irradiated rats, the second increase in SCE coincides with tumor growth, whereas the first increase might be due to DNA damage that was rapidly repaired.
authors.affiliation: Commissariat à l'Energie Atomique, IPSN, Département de Protection Sanitaire, Fontenay aux Roses, France.
authors.forename: J L
authors.initials: JL
authors.lastname: Poncy
authors.forename: P
authors.initials: P
authors.lastname: Fritsch
authors.forename: R
authors.initials: R
authors.lastname: Masse
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000528:radiation effects*; D009502:Neutrons; D051381:Rats; D011919:Rats, Inbred Strains; D012854:Sister Chromatid Exchange / Q000528:radiation effects*; D013997:Time Factors; D014916:Whole-Body Irradiation