title: Depletion of T cells from bone marrow for allogeneic transplantation: method for treatment of bone marrow in bulk.
abstract: T lymphocytes were depleted from donor marrow for 23 patients undergoing allogeneic bone marrow transplantation using an anti-T-cell antibody, CT-2, and complement. The methodology is described in detail for in vitro depletion of large quantities of bone marrow. The extent of T-lymphocyte depletion using various T-cell markers, the percent of marrow lost in the processing and quantity of antibody, and complement needed are presented. These techniques for in vitro T-lymphocyte depletion were reproducible and did result in an average final yield of 47% of the harvested donor marrow.
authors.forename: M E
authors.initials: ME
authors.lastname: Trigg
authors.forename: A
authors.initials: A
authors.lastname: Peterson
authors.forename: C
authors.initials: C
authors.lastname: Erickson
authors.forename: M
authors.initials: M
authors.lastname: Bozdech
authors.forename: R
authors.initials: R
authors.lastname: Billing
authors.forename: P
authors.initials: P
authors.lastname: Sondel
authors.forename: J
authors.initials: J
authors.lastname: Finlay
authors.forename: J
authors.initials: J
authors.lastname: Surfus
authors.forename: M
authors.initials: M
authors.lastname: Stuiber
authors.forename: R
authors.initials: R
authors.lastname: Hong
mesh_terms: D000911:Antibodies, Monoclonal / Q000627:therapeutic use; D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface / Q000032:analysis; D001853:Bone Marrow / Q000276:immunology; D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation; D003165:Complement System Proteins / Q000276:immunology; D003602:Cytotoxicity, Immunologic; D006801:Humans; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface; D003165:Complement System Proteins