[Platelet phenotyping and study of alloantibodies using flow cytometry].
In this study, we describe a flow cytometric technic for the detection and characterization of platelet allo antibodies and for platelets grouping in the platelet group PLA. This new technique is a variant of the platelet suspension immuno fluorescence test. It is rapid, simple, sensitive and specific. So it is very useful in the cases of neonatal thrombocytopenia and posttransfusion purpura. Moreover, anti-HLA antibodies don't obstruct the detection of anti-PLA1 antibodies.
Vergracht
A
A
Laboratoire d'Immunologie leuco-plaquettaire, CRTS, Nantes.
Valentin
N
N
Bignon
J D
JD
Patereau
C
C
Muller
J Y
JY
D016824:Antigens, Human Platelet; D001792:Blood Platelets / Q000032:analysis / Q000145:classification* / Q000276:immunology; D005434:Flow Cytometry / Q000379:methods; D005455:Fluorescent Antibody Technique; D006801:Humans; D039661:Integrin beta3; D007518:Isoantibodies / Q000032:analysis*; D007519:Isoantigens / Q000032:analysis* / Q000276:immunology; D010641:Phenotype
D016824:Antigens, Human Platelet; C061248:ITGB3 protein, human; D039661:Integrin beta3; D007518:Isoantibodies; D007519:Isoantigens
Phénotypage plaquettaire et recherche d'allo-anticorps en cytométrie de flux.