Construction of a plasmid for high-level expression of goat alpha-lactalbumin and its derivative in E. coli.
The high-level expression system of goat alpha-lactalbumin (alpha-LA) in E. coli was established by fusing the alpha-LA cDNA to porcine adenylate kinase cDNA and expressing the fused gene under the control of tac promoter. For high-level expression, elimination of 3'-noncoding region of the alpha-LA cDNA was found to be necessary.
Kumagai
I
I
Department of Industrial Chemistry, Faculty of Engineering, University of Tokyo, Japan.
Inaizumi
M
M
Tamaki
E
E
Misawa
S
S
Hibino
T
T
Miura
K
K
D000818:Animals; D003001:Cloning, Molecular / Q000379:methods; D004926:Escherichia coli / Q000235:genetics*; D005796:Genes; D006041:Goats; D007768:Lactalbumin / Q000235:genetics*; D010957:Plasmids
D007768:Lactalbumin