Fluorescence microscopic studies on mitochondria and mitochondrial nucleoids in a wild-type strain and respiratory mutants of Candida albicans.
A wild-type strain and two respiratory mutants of Candida albicans were examined for mitochondria and mitochondrial nucleoids (mt-nucleoids) using the fluorescent dyes, 2-(4-dimethylaminostyryl)-1-methylpyridinium iodide (DASPMI) and 4', 6-diamidino-2-phenylindole (DAPI). Rapidly growing cells of the wild type possessed one or a few giant branched mitochondria that were intensely stained with DASPMI. When a bud emerged, an end of the giant mitochondrion extended into the bud and the mitochondrion was divided and partitioned into mother and daughter cells by cytokinesis. Cell cycle-associated fragmentation or fusion of mitochondria were not demonstrated. The mutant KRD-8, that possesses cristate mitochondria but respires at a lower level, was shown to contain one or a few, less stainable giant mitochondria per cell. DASPMI failed to stain cells of the mutant KRD-19 which lacks cytochrome aa3 and cristate mitochondria. About eight and 10 mt-nucleoids were detected as discrete fluorescent spots in DAPI-stained cells of the wild type and KRD-8, respectively. KRD-19 cells also possessed mt-nucleoids, although the number of mt-nucleoids per cell seemed to be smaller than that of the wild type. In all the strains, mt-nucleoids existed discretely throughout the budding cycle, and the increase of their number per cell appeared to correlate with the cellular volume but not with nuclear division.
Ito-Kuwa
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S
General Research Institute, School of Dentistry, Nippon Dental University, Niigata, Japan.
Aoki
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S
Watanabe
T
T
Ehara
T
T
Osafune
T
T
D002176:Candida albicans / Q000166:cytology* / Q000235:genetics / Q000378:metabolism; D002455:Cell Division; D004734:Energy Metabolism; D007211:Indoles; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D008928:Mitochondria / Q000648:ultrastructure*; D009154:Mutation; D015388:Organelles / Q000648:ultrastructure*; D011726:Pyridinium Compounds
D007211:Indoles; D011726:Pyridinium Compounds; C011786:2-(4-(dimethylamino)styryl)-1-methylpyridinium; C007293:DAPI