Expression of CaMV ORF IV in Escherichia coli.
A CaMV DNA fragment corresponding to nucleotides 2200-3992 and including the coding sequence (2200-3670) of open reading frame IV was inserted in the pTG908 prokaryotic expression vector. In the recombinant pTG-IV plasmid, ORF IV, which codes for the coat protein precursor, was fused to the N-terminal coding sequence of the lambda CII gene, which is under transcriptional control of the lambda PL promoter. The expected fusion protein CII-ORF IV had a calculated molecular weight of 58.4 Kd. Nevertheless, temperature induction of the PL promoter resulted in synthesis of a major 76-Kd fusion protein: the coat protein precursor migrated abnormally on SDS polyacrylamide gel.
Albrecht
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H
Institut de Biologie Moléculaire des Plantes, Strasbourg, France.
Lebeurier
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G
D015153:Blotting, Western; D003001:Cloning, Molecular; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000235:genetics; D005814:Genes, Viral; D005822:Genetic Vectors; D008970:Molecular Weight; D009029:Mosaic Viruses / Q000235:genetics*; D011498:Protein Precursors / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics*
D004274:DNA, Recombinant; D011498:Protein Precursors; D014764:Viral Proteins