title: Purification and characterization of beef and pig liver aldehyde dehydrogenases.
abstract: Beef liver cytosolic, mitochondrial, and pig liver mitochondrial aldehyde dehydrogenases (ALDH) had been purified to homogeneity. The two mitochondrial enzymes as with other mammalian mitochondrial enzymes had properties very similar to that of the corresponding human enzyme. These include immunological as well as basic kinetic properties such as low Km for aldehyde, activation by Mg2+ ions, and lack of inhibition by disulfiram. A major difference between these two enzymes and the human mitochondrial enzyme was that they contained an N-terminal-blocked amino acid. Cytosolic ALDHs from human and horse liver have been shown to possess an N-acetyl serine as the N-terminal residue; beef cytosolic ALDH was also found to be blocked. Tissue preparations and subcellular fractions from beef or pig liver could be used to study acetaldehyde oxidation. This is the subject of the accompanying paper (Cao Q-N, Tu G-C, Weiner H, Alcohol Clin Exp Res 12:xxx-xxx, 1988).
authors.affiliation: Biochemistry Department, Purdue University, West Lafayette, Indiana 47907.
authors.forename: K L
authors.initials: KL
authors.lastname: Guan
authors.forename: Y K
authors.initials: YK
authors.lastname: Pak
authors.forename: G C
authors.initials: GC
authors.lastname: Tu
authors.forename: Q N
authors.initials: QN
authors.lastname: Cao
authors.forename: H
authors.initials: H
authors.lastname: Weiner
mesh_terms: D000444:Aldehyde Dehydrogenase / Q000037:antagonists & inhibitors / Q000302:isolation & purification*; D000596:Amino Acids / Q000302:isolation & purification; D000818:Animals; D002417:Cattle; D004221:Disulfiram / Q000494:pharmacology; D007527:Isoenzymes / Q000302:isolation & purification; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008274:Magnesium / Q000494:pharmacology; D008970:Molecular Weight; D013552:Swine
chemical_list: D000596:Amino Acids; D007527:Isoenzymes; D000444:Aldehyde Dehydrogenase; D008274:Magnesium; D004221:Disulfiram