Acetylcholine release from guinea pig caudate slices evoked by phorbol ester and calcium.
In guinea pig caudate slices preloaded with [3H]choline, superfusion with medium containing Ca2+ and the phorbol ester TPA (12-O-tetradecanoyl phorbol 13-acetate) produced an increase in the Ca2+-dependent, depolarization-evoked release of acetylcholine (ACh), with no significant effect on the evoked Ca2+ influx. The effect of TPA was antagonized by polymyxin B and H-7, both inhibitors of protein kinase C. The combination of TPA and calcium ionophore (A23187) provoked the release of ACh to a level equal to the maximal response induced by depolarizing stimuli. TPA alone had no significant effect on the release of ACh. These results suggest that protein kinase C probably plays a role in transmembrane signal transduction involved in the release of neurotransmitter from nerve terminals in the brain.
Tanaka
C
C
Fujiwara
H
H
Fujii
Y
Y
D000109:Acetylcholine / Q000378:metabolism*; D000634:Aminoquinolines; D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000494:pharmacology*; D002421:Caudate Nucleus / Q000378:metabolism*; D004357:Drug Synergism; D005260:Female; D006168:Guinea Pigs; D066298:In Vitro Techniques; D008297:Male; D010704:Phorbols / Q000494:pharmacology*; D011112:Polymyxin B / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D000634:Aminoquinolines; D010704:Phorbols; D000001:Calcimycin; C035738:Quin2-acetoxymethyl ester; D011112:Polymyxin B; D000109:Acetylcholine; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium