Curcumins as inhibitors of nitrosation in vitro.
The effects of turmeric extract and its pure yellow pigments curcumin I, II and III were tested on the nitrosation of methylurea by sodium nitrite at pH 3.6 and 30 degrees C. The nitrosomethylurea formed was monitored by checking the mutagenicity in S. typhimurium strains TA1535 and TA100 without metabolic activation. Turmeric extract as well as curcumins exhibit dose-dependent decreases of nitrosation. Curcumin III was the most effective nitrosation inhibitor among the compounds tested. The simultaneous treatment of inhibitor with nitrosation precursors was essential and pre- or post-treatment of inhibitor had no effect on the mutagenicity of nitrosomethylurea. The binding of nitrite with the inhibitors was studied at pH 3.6 and 30 degrees C. Curcumin I shows a dose-dependent depletion of nitrite ions thus making nitrite non-available for nitrosation. Curcumin I and III when tested also showed a time-dependent depletion of nitrite ions at pH 3.6 and 30 degrees C. Curcumin III has a higher affinity for nitrite ions than curcumin I.
Nagabhushan
M
M
Carcinogenesis Division, Cancer Research Institute, Tata Memorial Centre, Parel, Bombay, India.
Nair
U J
UJ
Amonkar
A J
AJ
D'Souza
A V
AV
Bhide
S V
SV
D002396:Catechols; D055598:Chemical Phenomena; D002621:Chemistry; D003474:Curcumin; D006863:Hydrogen-Ion Concentration; D008770:Methylnitrosourea; D008782:Methylurea Compounds; D009152:Mutagenicity Tests; D009573:Nitrites; D012486:Salmonella typhimurium / Q000187:drug effects; D012977:Sodium Nitrite; D013997:Time Factors
D002396:Catechols; D008782:Methylurea Compounds; D009573:Nitrites; D008770:Methylnitrosourea; D003474:Curcumin; D012977:Sodium Nitrite; C002352:methylurea