Rat sperm protamine. Isolation and sequence analysis.
Rat protamine was extracted from S-(pyridylethylated) epididymal sperm cell nuclei with dilute hydrochloric acid. The final purification was achieved by reversed-phase high-performance liquid chromatography. The primary structure was determined by N-terminal sequencing of the total S-(pyridylethylated) protein, and of endoproteinase Lys-C- and thermolysin-derived fragments. Rat protamine consists of 50 amino-acid residues. It is a typical type 1 protamine and differs in two and ten positions from the corresponding mouse and rabbit protamine, respectively. Only 26 positions are invariant in all type 1 mammalian protamines.
Ammer
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H
Max-Planck-Institut für Biochemie, Martinsried bei München.
Henschen
A
A
D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000302:isolation & purification; D010447:Peptide Hydrolases; D011479:Protamines / Q000302:isolation & purification*; D051381:Rats; D011919:Rats, Inbred Strains; D013045:Species Specificity; D013094:Spermatozoa / Q000032:analysis*
D010446:Peptide Fragments; D011479:Protamines; D010447:Peptide Hydrolases