title: Transforming growth factor beta-1 in acute myocardial infarction in rats.
abstract: TGF-beta 1 has been examined in the heart during myocardial infarction caused by ligation of the left coronary artery. Infarcted and uninfarcted myocardium have been compared by immunohistochemical staining of TGF-beta 1 and by Northern blot analysis of mRNA. Normal ventricular myocytes are strongly stained by an antibody to TGF-beta 1. Progressive loss of staining of these myocytes begins within 1 hr after coronary ligation. However, by 24-48 hr after ligation, intense staining of myocytes at the margin of infarcted areas is seen. Northern blots of infarcted myocardium 48 hr after ligation show a 3- to 4-fold increase in the principal 2.4 kb TGF-beta 1 mRNA; there is also a marked increase in a minor 1.9 kb transcript. In the same tissue samples, there is a 2-fold decrease in the mRNA for the glycolytic enzyme, glyceraldehyde-3-phosphate dehydrogenase. The results indicate a significant role for TGF-beta in the response of the heart to injury.
authors.affiliation: Laboratory of Chemoprevention, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892.
authors.forename: N L
authors.initials: NL
authors.lastname: Thompson
authors.forename: F
authors.initials: F
authors.lastname: Bazoberry
authors.forename: E H
authors.initials: EH
authors.lastname: Speir
authors.forename: W
authors.initials: W
authors.lastname: Casscells
authors.forename: V J
authors.initials: VJ
authors.lastname: Ferrans
authors.forename: K C
authors.initials: KC
authors.lastname: Flanders
authors.forename: P
authors.initials: P
authors.lastname: Kondaiah
authors.forename: A G
authors.initials: AG
authors.lastname: Geiser
authors.forename: M B
authors.initials: MB
authors.lastname: Sporn
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D003250:Constriction; D004195:Disease Models, Animal; D007124:Immunoenzyme Techniques; D008297:Male; D009203:Myocardial Infarction / Q000378:metabolism* / Q000473:pathology; D009206:Myocardium / Q000166:cytology; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D015291:Transforming Growth Factors / Q000235:genetics / Q000378:metabolism* / Q000502:physiology
chemical_list: D012333:RNA, Messenger; D015291:Transforming Growth Factors