The detection of a spectrin-like protein in Trypanosoma cruzi with a polyclonal antibody.
A rabbit serum raised against sheep erythrocyte spectrin stained mainly the flagella of epimastigote forms of the parasitic protozoan Trypanosoma cruzi. In Western blots of T. cruzi proteins solubilized with Nonidet P-40 it recognized mainly a polypeptide doublet with an apparent molecular weight of about 240 kDa, while a pre-immune rabbit serum and a tubulin monoclonal antibody did not. The results are consistent with the idea that a spectrin-like protein may be involved in cytoskeleton-membrane interactions in flagella of T. cruzi.
Alcina
A
A
Centro de Biologia Molecular (CSIC-UAM), Universidad Autonoma, Madrid, Spain.
Hargreaves
A J
AJ
Avila
J
J
Hesketh
J E
JE
Fresno
M
M
D000818:Animals; D000906:Antibodies / Q000276:immunology*; D003429:Cross Reactions; D003599:Cytoskeleton / Q000032:analysis; D005455:Fluorescent Antibody Technique; D015151:Immunoblotting; D008970:Molecular Weight; D013049:Spectrin / Q000032:analysis*; D014349:Trypanosoma cruzi / Q000032:analysis*
D000906:Antibodies; D013049:Spectrin