title: Immunoreactive prorenin and its profragment peptide are present in human juxtaglomerular cells.
abstract: Although prorenin appears to be activated through the cleavage of its prosegment in the juxtaglomerular cells, the presence of prosegment peptide has never been demonstrated. We therefore studied prorenin in juxtaglomerular tumour cells, both by immunohistochemistry and by radio-immunoassay. Synthetic peptide covering the 14-carboxyterminal sequence of human renin prosegment was used to prepare both antibody and radiolabelled tracer. Intense immunostaining of prorenin was demonstrated in the tumour cells. By gel filtration, however, immunoreactive prorenin was shown to consist of two major components. The first peak, located at the elution position of activatable inactive renin, was regarded as prorenin. The other peak was located at an elution position with a smaller molecular weight, which was assumed to represent the profragment. These results suggest that both prosegment peptide and prorenin are present in the juxtaglomerular cells.
authors.affiliation: Department of Medicine, Tokyo Women's Medical College, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Naruse
authors.forename: M
authors.initials: M
authors.lastname: Naruse
authors.forename: S
authors.initials: S
authors.lastname: Hirose
authors.forename: M
authors.initials: M
authors.lastname: Nagano
authors.forename: M
authors.initials: M
authors.lastname: Yamakado
authors.forename: F
authors.initials: F
authors.lastname: Kurimoto
authors.forename: H
authors.initials: H
authors.lastname: Sakurai
authors.forename: Z P
authors.initials: ZP
authors.lastname: Zeng
authors.forename: H
authors.initials: H
authors.lastname: Demura
authors.forename: T
authors.initials: T
authors.lastname: Inagami
mesh_terms: D000328:Adult; D004792:Enzyme Precursors / Q000378:metabolism*; D005260:Female; D006801:Humans; D007606:Juxtaglomerular Apparatus / Q000378:metabolism*; D007680:Kidney Neoplasms / Q000378:metabolism*; D008970:Molecular Weight; D012083:Renin / Q000378:metabolism*
chemical_list: D004792:Enzyme Precursors; D012083:Renin