Administration of a 600 kD molecular fraction from pancreatic islets suppresses immune mediated diabetes in mice.
Treatment of male C57BL6/J mice with low doses of streptozotocin causes the development of diabetes. Infiltration of pancreatic islets by immune cells and the concomitant loss of beta islet cells can be prevented by immunosuppression. We now report that i.v. administration of islet homogenate 13 d prior to low dose streptozotocin treatment largely protects from development diabetes. Homogenates from liver, kidney or exocrine pancreas were without effect. The suppressive activity was isolated partially from mouse islets as well as from a rat insulinoma cell line. After gelchromatography the suppressive activity was found in the 600 kD fraction. The relevant substance appears negatively charged and does not bind to lentil lectin or concanavalin A.
Kolb
H
H
Diabetes Research Institute, University of Düsseldorf, FRG.
Marx-Soho Moumé
C
C
Kiesel
U
U
D000818:Animals; D000941:Antigens / Q000032:analysis; D003921:Diabetes Mellitus, Experimental / Q000517:prevention & control*; D007340:Insulinoma / Q000276:immunology; D007515:Islets of Langerhans / Q000276:immunology*; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008970:Molecular Weight; D009928:Organ Specificity; D051381:Rats; D013154:Spleen / Q000166:cytology; D014407:Tumor Cells, Cultured
D000941:Antigens