Human insulin gene in transgenic mouse lines.
Transgenic mouse lines carrying the human insulin gene flanked by 4 kilobases (kb) in 5' and 5.5 kb in 3' were obtained. The presence of the human C-peptide in serum and urine, and of specific transcripts of the human gene in pancreas RNA indicate that the human DNA fragment contains the sequences necessary for correct phenotypic expression of the gene.
Fromont-Racine
M
M
Institut Jacques Monod du CNRS, Unité INSERM 257, Université Paris VII, France.
Bucchini
D
D
Desbois
P
P
Pictet
R
R
Jami
J
J
D000818:Animals; D015139:Blotting, Southern; D002096:C-Peptide / Q000097:blood; D004247:DNA / Q000008:administration & dosage / Q000235:genetics; D015342:DNA Probes; D005796:Genes; D006801:Humans; D007328:Insulin / Q000235:genetics*; D007515:Islets of Langerhans / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D008845:Microinjections; D011994:Recombinant Proteins / Q000097:blood; D014158:Transcription, Genetic
D002096:C-Peptide; D015342:DNA Probes; D007328:Insulin; D011994:Recombinant Proteins; D004247:DNA