title: Characterization of activated proto-oncogenes in chemically transformed Syrian hamster embryo cells.
abstract: The Syrian hamster embryo (SHE) cell transformation model has been used by many investigators to study the multistep process of neoplastic transformation induced by chemical carcinogens. In this study we have attempted to determine if activated proto-oncogenes are present in the transformed cells induced by a variety of chemical carcinogens. Twelve carcinogen-induced hamster cell lines, established by treatment of normal SHE cells with benzo[a]pyrene, diethylstilbestrol, or asbestos, were examined. One spontaneously transformed cell line (BHK-A) was also studied. Some of the cell lines were also tested for oncogene activation at the preneoplastic stage, before they acquired tumorigenic potential. DNAs from normal, preneoplastic, and neoplastic cells were tested by transfection into mouse NIH 3T3 cells, and morphologically transformed foci were scored on the contact-inhibited monolayer of 3T3 cells. The frequency of focus formation for normal SHE cell DNA was less than 0.0008 foci/microgram DNA, while approximately 40% (5 of 12) of the DNAs from carcinogen-induced, tumorigenic hamster cell lines induced foci at a frequency of greater than or equal to 0.012 foci/microgram DNA. The other seven carcinogen-induced cell lines and the BHK-A cells were negative (less than 0.002 foci/microgram DNA). When the DNAs from transformed foci induced by the five positive cell lines were retransfected into NIH 3T3 cells, the frequency of secondary foci of 3T3 cells was as much as 50-fold higher (1.34 foci/microgram DNA) than with the primary transfectants. DNAs from transformed foci or tumors derived from transformed foci were screened by Southern blot analyses with known oncogenes and with a hamster repetitive DNA probe for the presence of transfected hamster oncogenes.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Laboratory of Molecular Carcinogenesis, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709.
authors.forename: T M
authors.initials: TM
authors.lastname: Gilmer
authors.forename: L A
authors.initials: LA
authors.lastname: Annab
authors.forename: J C
authors.initials: JC
authors.lastname: Barrett
mesh_terms: D000818:Animals; D001194:Asbestos; D001564:Benzo(a)pyrene; D002461:Cell Line, Transformed; D002471:Cell Transformation, Neoplastic / Q000139:chemically induced*; D006224:Cricetinae; D004054:Diethylstilbestrol; D004622:Embryo, Mammalian / Q000166:cytology; D005786:Gene Expression Regulation; D011905:Genes, ras; D008647:Mesocricetus; D009857:Oncogenes; D011519:Proto-Oncogenes; D014162:Transfection
chemical_list: D001194:Asbestos; D001564:Benzo(a)pyrene; D004054:Diethylstilbestrol