title: cAMP-dependent protein kinase from Dictyostelium discoideum.
abstract: The cAMP-dependent protein kinase (cAK) from Dictyostelium discoideum is an enzyme composed of one catalytic and one regulatory subunit. Upon binding of cAMP, the holoenzyme dissociates to liberate free active catalytic subunits. The cAK is developmentally regulated, ranging from very little activity in vegetative cells to maximal expression in postaggregative cells. Although there is no immunological cross-reaction between the subunits of cAKs from Dictyostelium and from other organisms, they share several biochemical properties. A complete cDNA for the regulatory subunit has been cloned and sequenced. Only one copy of the gene for the regulatory subunit is present per haploid genome. On the basis of the comparison of the structure of the cAK from Dictyostelium with its counterparts in yeast and higher eukaryotes, we propose a model for the evolution of cyclic-nucleotide-binding proteins.
authors.affiliation: Unité de Biochimie Cellulaire, Institut Pasteur, Paris, France.
authors.forename: M
authors.initials: M
authors.lastname: Veron
authors.forename: R
authors.initials: R
authors.lastname: Mutzel
authors.forename: M L
authors.initials: ML
authors.lastname: Lacombe
authors.forename: M N
authors.initials: MN
authors.lastname: Simon
authors.forename: V
authors.initials: V
authors.lastname: Wallet
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D005075:Biological Evolution; D004023:Dictyostelium / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D005786:Gene Expression Regulation; D005796:Genes; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D011494:Protein Kinases / Q000235:genetics*
chemical_list: D011494:Protein Kinases