title: Changes in canine leukocyte glucocorticoid receptors during endotoxin shock.
abstract: We studied changes in canine leukocyte glucocorticoid receptors during endotoxin shock. Blood samples for analysis were collected and leukocytes were isolated just prior to and 2 and 6 hours after endotoxin administration. Employing 3H-dexamethasone (3H-Dex) as a ligand, we studied 3H-Dex-specific binding of the leukocytes in dogs and their changes during endotoxin shock. Results from two groups (anesthetized and conscious) showed that the specific binding of the leukocytes decreased significantly 2 hours after endotoxin administration in both groups and 6 hours after endotoxin in anesthetized dogs. In conscious dogs, the specific binding returned to normal by 6 hours. No correlation was found between the changes of serum cortisol and 3H-Dex-specific binding. It may be suggested that perturbations in glucocorticoid hormone action at the receptor level might be involved in the pathogenesis of endotoxin shock.
authors.affiliation: Department of Pathophysiology, Second Military Medical University, Shanghai, People's Republic of China.
authors.forename: F
authors.initials: F
authors.lastname: Li
authors.forename: R B
authors.initials: RB
authors.lastname: Xu
mesh_terms: D000818:Animals; D004285:Dogs; D004731:Endotoxins; D006439:Hemodynamics; D006854:Hydrocortisone / Q000097:blood; D007962:Leukocytes / Q000378:metabolism*; D008297:Male; D011965:Receptors, Glucocorticoid / Q000378:metabolism*; D012706:Serratia marcescens; D012772:Shock, Septic / Q000209:etiology / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology
chemical_list: D004731:Endotoxins; D011965:Receptors, Glucocorticoid; D006854:Hydrocortisone