A specific and efficient photoreaction between E. coli RNA polymerase and T+1 in the lacUV5 or deoP1 promoter.
Upon irradiation of the RNA polymerase-lacUV5 or deoP1 promoter complex with short wavelength ultraviolet light (lambda less than or equal to 300 nm) the polymerase is covalently crosslinked at an efficiency of greater than 10% to the first transcribed base of the template DNA strand when this is a thymine. The temperature dependence of this RNA polymerase-T+1 photoreaction strongly indicates a relation to the formation of the open complex. It is suggested that open complex formation is preceded or accompanied by a specific contact between the RNA polymerase and the first transcribed base of the DNA template.
Jeppesen
C
C
Department of Biochemistry B, University of Copenhagen, Denmark.
Jensen
K F
KF
Nielsen
P E
PE
D003432:Cross-Linking Reagents; D004247:DNA / Q000528:radiation effects; D012321:DNA-Directed RNA Polymerases / Q000235:genetics* / Q000528:radiation effects; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics* / Q000528:radiation effects; D007763:Lac Operon / Q000528:radiation effects*; D010777:Photochemistry; D011401:Promoter Regions, Genetic / Q000528:radiation effects*; D013698:Templates, Genetic; D013941:Thymine / Q000528:radiation effects*; D014158:Transcription, Genetic / Q000528:radiation effects; D014466:Ultraviolet Rays
D003432:Cross-Linking Reagents; D004247:DNA; D012321:DNA-Directed RNA Polymerases; D013941:Thymine