Glucose-6-phosphate dehydrogenase activity of the malaria parasite Plasmodium falciparum.
Schizonts of Plasmodium falciparum, grown either in normal or glucose-6-phosphate dehydrogenase (G6PDH) deficient human red cells, contain an electrophoretically slow-moving form of G6PDH. The slow mobility of the G6PDH in non-dissociating polyacrylamide gels is due to its large size (Mr ca. 450,000) rather than to its charge. The activity of this enzyme was less than 10% of normal red cell G6PDH. These characteristics of the parasite-associated G6PDH were unaltered when parasites were grown in red cells from a G6PDH A+B+ heterozygote or following the introduction of a heterologous G6PDH into resealed ghosts. Differential absorption of the parasite-associated and red cell G6PDHs was demonstrated with antisera containing antibodies to red cell G6PDH. These studies show that a novel form of G6PDH is associated with P. falciparum in normal red cells without the requirements for induction by one or several cycles of multiplication in G6PDH deficient red cells.
Ling
I T
IT
National Institute for Medical Research, London, U.K.
Wilson
R J
RJ
D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D004910:Erythrocyte Membrane / Q000469:parasitology; D004912:Erythrocytes / Q000469:parasitology; D005954:Glucosephosphate Dehydrogenase / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D007525:Isoelectric Focusing; D008288:Malaria / Q000201:enzymology; D051379:Mice; D010963:Plasmodium falciparum / Q000201:enzymology*; D012503:Saponins; D013379:Substrate Specificity
D012503:Saponins; D005954:Glucosephosphate Dehydrogenase