Immunophenotyping of leukaemias by flow cytometry and APAAP: a two-year comparative analysis in hospital practice.
Using a panel of monoclonal antibodies for the immunophenotyping of haematological malignancies, we have made a direct comparison of the usefulness of indirect immunofluorescence using flow cytometry (IFC) alongside the alkaline phosphatase-anti-alkaline phosphatase (APAAP) immunoenzyme method. Analysis of 400 consecutive patient samples (blood and bone marrow) over a 2-year period, resulted in unequivocal phenotyping agreement by both methods in 98 per cent of cases. The positive results accounting for 2 per cent discordance were obtained by the APAAP method. The value of the technique in the clinical management of patients with leukaemia is documented together with guidelines for clinical laboratories.
Sewell
H F
HF
Department of Pathology, Aberdeen Royal Infirmary, U.K.
Milton
J I
JI
MacKenzie
R
R
King
D J
DJ
Dawson
A A
AA
Lessels
S E
SE
Bennett
B
B
Davidson
R L
RL
Walker
F
F
D000293:Adolescent; D000368:Aged; D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation; D014408:Biomarkers, Tumor / Q000276:immunology*; D003951:Diagnostic Errors; D005069:Evaluation Studies as Topic; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006801:Humans; D007124:Immunoenzyme Techniques; D007938:Leukemia / Q000145:classification / Q000175:diagnosis* / Q000276:immunology; D008297:Male; D010641:Phenotype
D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation; D014408:Biomarkers, Tumor