title: Meiotic breakdown of nuclear envelope in oocytes of Spisula solidissima involves phosphorylation and release of nuclear lamin.
abstract: During meiotic nuclear envelope breakdown (NEBD) in maturing oocytes of the surf clam, Spisula solidissima, the 67-kDa lamin is extensively phosphorylated, concurrently with its solubilization. This is accompanied by a reduction of the nuclear diameter. Quercetin, a protein kinase inhibitor, does not affect lamin phosphorylation and release, nor NEBD per se, but specifically inhibits the early phosphorylation of a set of proteins, on which NEBD seems to depend. Our results suggest that meiotic NEBD in Spisula oocytes may be controlled by a mechanism which involves lamin phosphorylation, similar to that which is thought to operate in mitosis.
authors.affiliation: Department of Cell Biology and Anatomy, Northwestern University Medical School, Chicago, Illinois 60611.
authors.forename: G
authors.initials: G
authors.lastname: Dessev
authors.forename: R
authors.initials: R
authors.lastname: Goldman
mesh_terms: D000818:Animals; D049872:Bivalvia; D015180:Electrophoresis, Gel, Two-Dimensional; D007158:Immunologic Techniques; D007526:Isoelectric Point; D034882:Lamins; D008540:Meiosis; D008970:Molecular Weight; D009685:Nuclear Envelope / Q000502:physiology*; D009687:Nuclear Proteins / Q000502:physiology*; D009865:Oocytes / Q000502:physiology*; D010750:Phosphoproteins / Q000378:metabolism; D010766:Phosphorylation; D011794:Quercetin / Q000494:pharmacology; D012995:Solubility
chemical_list: D034882:Lamins; D009687:Nuclear Proteins; D010750:Phosphoproteins; D011794:Quercetin