Malignant transformation of rat liver cells by N-benzoyloxy-3'-methyl-4-methylaminoazobenzene and its related compound.
N-Benzoyloxy-3'-methyl-4-methylaminoazobenzene-(N-benzoyloxy-3'-Me-MAB) was highly toxic to rat liver epithelial cells (Ac2F) and induced many more chromosome aberrations and unscheduled DNA synthesis than did 3'-methyl-4-dimethylaminoazobenzene (3'-Me-DAB). N-Benzoyloxy-3'-Me-MAB and its related compound, N-acetoxy-4-methylaminoazobenzene, efficiently induced malignant transformation of the cells, but 3'-Me-DAB was not so effective. The cells transformed by N-acyloxy-4-methylaminoazobenzene (N-acyloxy-MAB) derivatives showed significant increases in plating efficiency in liquid medium and in the size of aggregates in rotation culture. Such increases were not seen in the 3'-Me-DAB-transformed cells. The results indicate that the N-acyloxy form of the 4-dimethylaminobenzene derivatives seems very likely to be the ultimate metabolite.
Tokiwa
T
T
Hishinuma
T
T
Degawa
M
M
Hashimoto
Y
Y
Sato
J
J
D000818:Animals; D002273:Carcinogens; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D004260:DNA Repair; D008099:Liver / Q000187:drug effects* / Q000473:pathology / Q000648:ultrastructure; D008749:Methyldimethylaminoazobenzene / Q000633:toxicity; D051381:Rats; D004124:p-Dimethylaminoazobenzene / Q000031:analogs & derivatives* / Q000633:toxicity
D002273:Carcinogens; D008749:Methyldimethylaminoazobenzene; C047575:N-benzoyloxy-3'-methyl-4-methylaminoazobenzene; D004124:p-Dimethylaminoazobenzene