The antimetastatic function of concomitant antitumor immunity. I. Host Ly-1+2+ effector T cells prevent the enumeration of metastatic tumor cells in a biological assay.
A mouse survival assay was evaluated for its suitability to enumerate metastatic P815 tumor cells in the draining lymph node and spleen of a B6D2 F1 (H-2b X H-2d) host bearing a primary intradermal P815 tumor. The mouse survival assay is based on the linear relationship between the log10 number of P815 tumor cells (H-2d) injected i.p. into mice and their mean survival time. It was found that the assay is capable of quantifying as few as 10 tumor cells in lymph node and spleen, but only if cell suspensions of these organs are treated with anti-H-2b serum and complement, in order to selectively destroy H-2bd host cells. This was necessary because host cells from the lymph node and spleen of a tumor-bearing host possessed antitumor functions, in that they were capable of destroying the H-2d P815 tumor cells when admixed with the tumor cells and injected i.p. into 800-rad irradiated test recipients. The kinetics of acquisition and loss of host cells with antitumor function and the Ly phenotype of these host cells suggest that they are the same cells that give the tumor-bearing host the capacity to express concomitant immunity against a tumor implant.
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D000818:Animals; D000950:Antigens, Ly / Q000276:immunology; D002455:Cell Division; D002460:Cell Line; D003601:Cytotoxicity Tests, Immunologic / Q000379:methods; D007958:Leukocyte Count; D008207:Lymphatic Metastasis; D008213:Lymphocyte Activation; D012515:Mast-Cell Sarcoma / Q000276:immunology* / Q000401:mortality / Q000503:physiopathology / Q000628:therapy; D051379:Mice; D008811:Mice, Inbred DBA; D010641:Phenotype; D013160:Splenic Neoplasms / Q000276:immunology / Q000556:secondary; D013602:T-Lymphocytes, Cytotoxic / Q000145:classification / Q000276:immunology*
D000950:Antigens, Ly