HTLV-III serology in hemophilia: relationship with immunologic abnormalities.
We investigated the relationship of the presence of antibodies to HTLV-III and immunologic abnormalities in patients with hemophilia. Serum antibodies to HTLV-III were analyzed by ELISA assay, immunoprecipitation of labeled cell extracts, and immunoprecipitation of purified HTLV-III p24. Thirty-four (61%) of the total group (n = 56) had antibody to HTLV-III; 34 (76%) of 45 patients given commercial factor VIII preparations were seropositive, compared with none of 11 patients treated exclusively with cryoprecipitate obtained from volunteer blood donors. Of patients who were seropositive for HTLV-III antibody, 94% had abnormal T4/T8 ratios, and 33% of those whose serum was antibody negative had abnormal T4/T8 ratios; five patients, each antibody positive, have lymphadenopathy syndrome. Sequential studies in a subset of patients indicate that there is a changing pattern of antibody production to HTLV-III antigens after seroconversion.
Gill
J C
JC
Menitove
J E
JE
Anderson
P R
PR
Casper
J T
JT
Devare
S G
SG
Wood
C
C
Adair
S
S
Casey
J
J
Scheffel
C
C
Montgomery
R R
RR
D000163:Acquired Immunodeficiency Syndrome / Q000150:complications; D000914:Antibodies, Viral / Q000032:analysis*; D000917:Antibody Formation; D002478:Cells, Cultured; D003450:Cryoglobulins / Q000627:therapeutic use; D004340:Drug Contamination; D004797:Enzyme-Linked Immunosorbent Assay; D005169:Factor VIII / Q000627:therapeutic use; D015483:HIV Antibodies; D006467:Hemophilia A / Q000276:immunology* / Q000628:therapy; D006801:Humans; D007457:Iodine Radioisotopes; D007958:Leukocyte Count; D008297:Male; D010976:Platelet Count; D013601:T-Lymphocytes
D000914:Antibodies, Viral; D003450:Cryoglobulins; D015483:HIV Antibodies; D007457:Iodine Radioisotopes; D005169:Factor VIII