Lactate observation in vivo by spectral editing in real time.
We have developed a novel in vivo proton MR spectroscopy magnetization transfer method for detection of lactate in ischemic tissue in the presence of interfering fat proton resonances. Pyruvate is magnetically labeled with a saturation pulse and, when converted to lactate, the lactate retains the label. Difference of spectra obtained with and without a saturation pulse contain no fat resonances. High-resolution spectra (determined with a GE 1.5 T Signa) of low lactate levels were obtained in vivo by water suppression using a 2662 composite RF pulse and slice-selective gradients. Spectral subtraction was performed in real time allowing the monitoring of a buildup of the intensity of the lactate peak. Pyruvate-lactate saturation transfer techniques should find wide applicability in the study of ischemia.
Vinitski
S
S
Department of Radiology, Thomas Jefferson University Hospital, Philadelphia, PA 19107.
Griffey
R H
RH
Szumowski
J
J
Matwiyoff
N A
NA
D006801:Humans; D007511:Ischemia / Q000378:metabolism; D007773:Lactates / Q000378:metabolism*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008961:Models, Structural; D011773:Pyruvates; D013382:Subtraction Technique
D007773:Lactates; D011773:Pyruvates