A simplified method for fixation of human and mouse preimplantation embryos which facilitates G-banding and karyotypic analysis.
A reliable method for the fixation of large numbers of preimplantation embryos was developed to allow application of routine G-banding methods to preimplantation stage embryos. The technique resulted in minimal loss of embryos and was suitable for both 1-cell mouse embryos and multi-pronuclear human embryos. Application of this method will increase the information gained from chromosome studies of embryonic development.
Roberts
C G
CG
Human Reproduction Unit, Royal North Shore Hospital, St Leonards, N.S.W., Australia.
O'Neill
C
C
D000818:Animals; D001755:Blastocyst / Q000166:cytology*; D002871:Chromosome Banding / Q000379:methods*; D003584:Cytological Techniques; D005260:Female; D006801:Humans; D007621:Karyotyping / Q000379:methods; D051379:Mice