Analysis of Salmonella lipopolysaccharides by sodium deoxycholate-polyacrylamide gel electrophoresis.
Lipopolysaccharides (LPSs) were analyzed by polyacrylamide gel electrophoresis (PAGE) in the presence of sodium deoxycholate (DOC) or sodium dodecyl sulphate (SDS) and the results obtained compared. Two-dimensional PAGE of the S form of LPS revealed that, with SDS, bands which appeared to be single after the one-dimensional experiment were resolved into several bands after two-dimensional electrophoresis. In the presence of DOC, however, a diagonal of single bands was obtained in one-dimensional electrophoresis indicating optimum resolution. The high quality of resolution by DOC-PAGE was constant for amounts of LPS up to 20 micrograms. Finally, DOC-PAGE does not require boiling of the samples in DOC, which may be an advantage over SDS-PAGE.
Komuro
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Max-Planck-Institut für Immunbiologie, Freiburg, F.R.G.
Galanos
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C
D003840:Deoxycholic Acid; D004591:Electrophoresis, Polyacrylamide Gel; D008070:Lipopolysaccharides / Q000032:analysis*; D008970:Molecular Weight; D012486:Salmonella typhimurium / Q000032:analysis
D008070:Lipopolysaccharides; D003840:Deoxycholic Acid