Cloning, sequencing and expression of the lipase gene from Pseudomonas fragi IFO-12049 in E. coli.
The lipase gene from Pseudomonas fragi IFO-12049 was isolated using the expression library and the primary structure of lipase deduced from the nucleotide sequence was determined. It is composed of 277 amino acid residues and a protein of Mr 29,966, which was close to the value of the lipase expressed in E. coli.
Aoyama
S
S
Research Center, Chisso Petrochemical Corporation, Chiba, Japan.
Yoshida
N
N
Inouye
S
S
D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D005786:Gene Expression Regulation; D008049:Lipase / Q000235:genetics*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D011549:Pseudomonas / Q000201:enzymology / Q000235:genetics*
D004269:DNA, Bacterial; D008049:Lipase