title: Interaction of the release factor with the Escherichia coli ribosome: inhibition of the 30S subunit domain by specific antibodies.
abstract: A domain of the 30S subunit of the Escherichia coli ribosome is in close contact with the release factor when it binds to the 70S particle during the termination of protein biosynthesis. This has been characterised using antibodies specific for the individual proteins of the small ribosomal subunit. Most antibodies do not affect the release factor-mediated reactions but those against S3, S4, S5 and S10 are inhibitory. These proteins are clustered on the lower head and the upper part of the small lobe of the subunit. The regions of these features which are near the interface between the two subunits in the 70S ribosome are known to be close to the base of the stalk of the 50S subunit.
authors.affiliation: Department of Biochemistry, University of Otago, Dunedin, New Zealand.
authors.forename: W P
authors.initials: WP
authors.lastname: Tate
authors.forename: K K
authors.initials: KK
authors.lastname: McCaughan
authors.forename: B
authors.initials: B
authors.lastname: Kastner
authors.forename: C N
authors.initials: CN
authors.lastname: Trotman
authors.forename: M
authors.initials: M
authors.lastname: Stoffler-Meilicke
authors.forename: G
authors.initials: G
authors.lastname: Stoffler
mesh_terms: D000918:Antibody Specificity; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000276:immunology / Q000378:metabolism; D001666:Binding Sites, Antibody; D004926:Escherichia coli / Q000378:metabolism*; D007074:Immunoglobulin G; D010443:Peptide Chain Termination, Translational; D010454:Peptide Termination Factors / Q000378:metabolism*; D012269:Ribosomal Proteins / Q000276:immunology / Q000378:metabolism; D012270:Ribosomes / Q000276:immunology / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D007074:Immunoglobulin G; D010454:Peptide Termination Factors; D012269:Ribosomal Proteins; C040469:ribosomal protein S30