title: Isolation and characterization of a mouse interleukin cDNA clone that expresses B-cell stimulatory factor 1 activities and T-cell- and mast-cell-stimulating activities.
abstract: A cDNA sequence coding for a unique mouse interleukin that expresses B-cell-, T-cell, and mast-cell-stimulating activities has been isolated from a mouse helper T-cell cDNA library. The library, constructed in the pcD expression vector, was screened by transfecting COS monkey cells with DNA pools to express the products encoded by full-length cDNA inserts. By assaying the transfected cell supernatants, we identified clones encoding a factor that stimulates T-cell and mast cell lines. This factor also induces Ia expression on resting B cells and enhances IgG1 and IgE production by B cells, two properties of B-cell-stimulatory factor 1. The DNA sequence codes for a polypeptide of 140 amino acid residues including a putative signal peptide. These results demonstrate that a single cDNA clone distinct from interleukin 2 and interleukin 3 encodes a polypeptide with multiple biological activities.
authors.forename: F
authors.initials: F
authors.lastname: Lee
authors.forename: T
authors.initials: T
authors.lastname: Yokota
authors.forename: T
authors.initials: T
authors.lastname: Otsuka
authors.forename: P
authors.initials: P
authors.lastname: Meyerson
authors.forename: D
authors.initials: D
authors.lastname: Villaret
authors.forename: R
authors.initials: R
authors.lastname: Coffman
authors.forename: T
authors.initials: T
authors.lastname: Mosmann
authors.forename: D
authors.initials: D
authors.lastname: Rennick
authors.forename: N
authors.initials: N
authors.lastname: Roehm
authors.forename: C
authors.initials: C
authors.lastname: Smith
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology*; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D006133:Growth Substances / Q000235:genetics* / Q000502:physiology; D007376:Interleukin-2 / Q000235:genetics* / Q000502:physiology; D007377:Interleukin-3; D015847:Interleukin-4; D008222:Lymphokines / Q000235:genetics* / Q000502:physiology; D008407:Mast Cells / Q000166:cytology*; D051379:Mice; D013601:T-Lymphocytes / Q000166:cytology / Q000502:physiology*
chemical_list: D006133:Growth Substances; D007376:Interleukin-2; D007377:Interleukin-3; D008222:Lymphokines; D015847:Interleukin-4; D004247:DNA