Use of human alpha-satellite deoxyribonucleic acid to detect Y-specific centromeric sequences.
The Y alphoid deoxyribonucleic acid probe Y97 has proved to be specific for the human Y centromere and to define a Y-specific 5.5 kb Eco RI fragment. Three experiments were designed to evaluate the sensitivity and the specificity of this Y alphoid probe Y97. In the first experiment the centromeric Y-specific 5.5 kb Eco RI fragment was clearly seen in the mixture of 0.050 microgram of male DNA with 4.950 micrograms of female DNA (1%). In the second experiment the same dilutional study was applied to the Yq11-related probe 4B-2 for comparison purpose. In the third experiment, hybridization with the Y97 probe was performed on 20 subjects with mosaic cell lines containing a cytogenetically identifiable Y (n = 10) and a cytogenetically unidentifiable minute (n = 10) fragment. Nineteen of the 20 subjects demonstrated the Y-specific 5.5 kb Eco RI hybridization band with the centromeric Y97 probe. These experiments demonstrated the utility of the Y97 probe to consistently identify cytogenetically altered Y chromosome fragments and confirm the mapping of the alphoid repeat sequences to the centromeric region of the Y chromosome.
Tho
S P
SP
Department of Obstetrics and Gynecology, Medical College of Georgia, Augusta 30912.
Behzadian
A
A
Byrd
J R
JR
McDonough
P G
PG
D001483:Base Sequence; D002503:Centromere / Q000032:analysis*; D002875:Chromosomes / Q000032:analysis*; D015342:DNA Probes; D004276:DNA, Satellite; D004587:Electrophoresis, Agar Gel; D005260:Female; D006801:Humans; D008297:Male; D009693:Nucleic Acid Hybridization; D012680:Sensitivity and Specificity; D012729:Sex Chromosome Aberrations / Q000175:diagnosis* / Q000235:genetics; D014998:Y Chromosome / Q000032:analysis*
D015342:DNA Probes; D004276:DNA, Satellite