Biological and immunological properties of the HMB-2 human melanoma cell line.
The HMB-2 human melanoma cell line derived from a lymph node metastatis is described. After long-term cultivation in vitro the cells retained their morphology, high growth rate, xenotransplantability into immunosuppressed mice and genotypic and phenotypic markers of human melanoma cells. Upon infection of the HMB-2 cells with temperature-sensitive mutant vesicular stomatitis virus (VSVts045) at nonpermissive temperature, a complemented virus pseudotype (VSV(HMB-2] was produced carrying assembled melanoma-associated antigens. Monoclonal antibodies prepared against HMB-2 cell membrane proteins and proteins of purified VSV(HMB-2) particles showed different reactivity with various human tumor cell lines and tissues: While the RG-12 anti-HMB-2 monoclonal antibody recognized a class II tumor-associated antigen present in melanoma and carcinoma tissues, the B-6 anti-VSV(HMB-2) antibody showed selective reactivity with melanoma cells.
Svec
J
J
Cancer Research Institute, Slovak Academy of Sciences, Bratislava, Czechoslovakia.
Veselovská
Z
Z
Keszeghová
V
V
Matoska
J
J
Marchetti
A
A
Squartini
F
F
D000818:Animals; D002455:Cell Division; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D007621:Karyotyping; D008207:Lymphatic Metastasis; D008545:Melanoma / Q000235:genetics / Q000276:immunology / Q000473:pathology*; D051379:Mice; D008808:Mice, Inbred CBA; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000302:isolation & purification; D009368:Neoplasm Transplantation; D011863:Radioimmunoassay; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured
D009363:Neoplasm Proteins