title: The bottom and top system: a new technique for blood component preparation and storage.
abstract: A new, automated technique for the preparation of blood components is described. A system of 3 or 4 integrally connected plastic containers (Optipac) is handled by a new type of extractor (Optipress). The container in which the blood is collected has an outlet at the top and another at the bottom. After normal centrifugation to obtain separation of the blood components, these are squeezed out from the top and bottom simultaneously under control of a photocell. The primary separation step results in three components: a leukocyte-poor red-cell suspension in SAGM medium, CPD plasma, and a buffy-coat preparation. The system has been tested in two laboratories (lab A and lab B). A 'heavy-spin' centrifugation to obtain a maximum yield of cell-poor plasma gave the best removal of leukocytes from the red cells; the remaining leukocyte content was 0.46 +/- 0.25 (lab A) and 0.5 +/- 0.4 (lab B) x 10(9)/red-cell unit. Platelet concentrates can be prepared either the normal way via platelet-rich plasma or from buffy coat. Red-cell 24-hour autologous posttransfusion survival using labeling with 51Cr was 87.5 +/- 4.1% (lab A) after 35 days, and 84.2 +/- 4.2% (lab A) and 77.5 +/- 1.5% (lab B) after 42 days. Red-cell morphology and fluidity compared favorably to previous studies using the same additive solution in traditional plastic-bag systems. The total adenine nucleotide concentration was maintained normal for 42 days.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Blood Center, University Hospital, Uppsala, Sweden.
authors.forename: C F
authors.initials: CF
authors.lastname: Högman
authors.forename: L
authors.initials: L
authors.lastname: Eriksson
authors.forename: K
authors.initials: K
authors.lastname: Hedlund
authors.forename: J
authors.initials: J
authors.lastname: Wallvik
mesh_terms: D019794:2,3-Diphosphoglycerate; D000249:Adenosine Monophosphate / Q000032:analysis; D001772:Blood Cell Count; D001786:Blood Glucose / Q000378:metabolism; D001793:Blood Preservation / Q000379:methods*; D004163:Diphosphoglyceric Acids / Q000032:analysis; D004905:Erythrocyte Aging; D004912:Erythrocytes; D005344:Fibrinopeptide A / Q000378:metabolism; D006461:Hemolysis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007610:Kallikreins / Q000378:metabolism; D010447:Peptide Hydrolases / Q000378:metabolism; D010949:Plasma; D065227:Transfusion Reaction
chemical_list: D001786:Blood Glucose; D004163:Diphosphoglyceric Acids; D019794:2,3-Diphosphoglycerate; D005344:Fibrinopeptide A; D000249:Adenosine Monophosphate; D010447:Peptide Hydrolases; D007610:Kallikreins