Effects of taurodeoxycholate on in vivo water and solute transport in rat jejunum in absence and presence of calcium.
Bile acids and fatty acids enhance the permeability of brush-border membrane vesicles for calcium. It has been postulated that increased influx of calcium into the enterocyte might be responsible for the fluid secretion induced by dihydroxy bile acids and fatty acids. During in vivo perfusion studies of the rat jejunum, 15 mM taurodeoxycholate induced secretion of electrolytes and water (P less than 0.001), reduced glucose absorption (P less than 0.001), and enhanced the absorption of mannitol (P less than 0.0125) and calcium (P less than 0.001). Calcium absorption continued to be enhanced during perfusion of a CaCl2-containing solution following the perfusion with taurodeoxycholate (P less than 0.05). In view of the previously demonstrated enhanced permeability of the apical brush-border membrane in the presence of bile acids, it is very likely that some calcium enters the enterocyte along the steep concentration gradient in the presence of taurodeoxycholate. In spite of enhanced calcium absorption, 15 mM CaCl2 had no effect on control absorption rates or on fluid secretion induced by taurodeoxycholate. The data indicate that the effects of bile acids on intestinal transport are not mediated by an influx of calcium into the enterocyte.
Ammon
H V
HV
Cho
D S
DS
Loeffler
R L
RL
Reetz
K L
KL
D000042:Absorption; D000818:Animals; D001647:Bile Acids and Salts / Q000502:physiology; D001692:Biological Transport; D002118:Calcium / Q000378:metabolism / Q000494:pharmacology*; D003840:Deoxycholic Acid / Q000031:analogs & derivatives*; D007583:Jejunum / Q000378:metabolism*; D008297:Male; D008353:Mannitol / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012996:Solutions; D013657:Taurodeoxycholic Acid / Q000494:pharmacology*; D014867:Water / Q000378:metabolism*
D001647:Bile Acids and Salts; D012996:Solutions; D003840:Deoxycholic Acid; D014867:Water; D008353:Mannitol; D013657:Taurodeoxycholic Acid; D002118:Calcium