title: Tumorigenic transformation of mouse NIH 3T3 cells by transfection with plasmid pSV2neo.
abstract: This study reports high incidence tumourigenic activity of the pSV2neo plasmid demonstrated by transfection of NIH 3T3 cells. The plasmid is frequently used as a dominant selectable marker for confirming successful gene transfection, and the findings indicate a need for caution in interpretation and design of assays for oncogenes which use co-transfection and subsequent selection with neomycin.
authors.affiliation: Nuffield Department of Pathology, University of Oxford, John Radcliffe Hospital, U.K.
authors.forename: J S
authors.initials: JS
authors.lastname: Wallace
authors.forename: K A
authors.initials: KA
authors.lastname: Fleming
authors.forename: D
authors.initials: D
authors.lastname: Tarin
mesh_terms: D000818:Animals; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000473:pathology*; D002999:Clone Cells; D004279:DNA, Viral; D004352:Drug Resistance, Microbial / Q000235:genetics; D011905:Genes, ras; D051379:Mice; D009355:Neomycin; D010957:Plasmids; D014162:Transfection
chemical_list: D004279:DNA, Viral; D009355:Neomycin