title: Leucine-tRNA ligase complexes.
abstract: The methodologies described in this chapter allow the reproducible preparation of native high-molecular-weight synthetase complexes of leuRL. These complexes have the ability to preferentially utilize extracellular leucine immediately upon transport and are likely the forms of the enzyme most important in the utilization of leucine for protein synthesis.
authors.forename: A
authors.initials: A
authors.lastname: Hampel
authors.forename: R
authors.initials: R
authors.lastname: Tritz
mesh_terms: D000604:Amino Acyl-tRNA Synthetases / Q000032:analysis*; D000818:Animals; D002460:Cell Line; D007700:Kinetics; D007930:Leucine; D007935:Leucine-tRNA Ligase / Q000032:analysis* / Q000378:metabolism; D011485:Protein Binding; D011865:Radioisotope Dilution Technique; D013816:Thermodynamics; D014316:Tritium
chemical_list: D014316:Tritium; D000604:Amino Acyl-tRNA Synthetases; D007935:Leucine-tRNA Ligase; D007930:Leucine