Studies on the major common precipitating antigen of capripoxvirus.
The proteins of sheep pox, goat pox, sheep and goat pox and lumpy skin disease (Neethling) viruses were labelled with [35S]methionine. The major structural polypeptides of these viruses co-migrated on polyacrylamide gels, demonstrating the very close biochemical relationship between them. Using the agar gel immunodiffusion (AGID) test with radiolabelled antigen preparations, a major common precipitating antigen was identified. This co-migrated on polyacrylamide gels with one of the major structural polypeptides [mol. wt. 67000 (67K)]. The use of [35S]methionine-labelled antigen preparations considerably improved the sensitivity of the AGID test as a diagnostic test for capripoxvirus antibody detection.
Kitching
R P
RP
Hammond
J M
JM
Black
D N
DN
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D002478:Cells, Cultured; D006041:Goats; D005779:Immunodiffusion; D008167:Lumpy skin disease virus / Q000032:analysis; D008297:Male; D008715:Methionine / Q000378:metabolism; D011212:Poxviridae / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification; D012756:Sheep; D013045:Species Specificity; D013462:Sulfur Radioisotopes; D013737:Testis; D014764:Viral Proteins / Q000032:analysis*
D000956:Antigens, Viral; D013462:Sulfur Radioisotopes; D014764:Viral Proteins; D008715:Methionine