Induction of follicle stimulating hormone receptor by erythroid differentiation factor on rat granulosa cell.
Erythroid differentiation factor (EDF), inhibin beta A-homodimer, induced expression of follicle stimulating hormone receptors on rat granulosa cells prepared from diethylstilbestrol primed immature female rats. After 3 day incubation with EDF, the number of FSH receptors on the granulosa cells was increased to about 3.5 times of the control value in a dose dependent manner with an ED50 value of 61 ng/ml. On the other hand, EDF related peptides, i.e., bovine 32K Da inhibin A and TGF beta, had no effect on the FSH receptor induction. The present observation suggests that EDF may play a role in the initiation of the cytodifferentiation of ovarian granulosa cells.
Hasegawa
Y
Y
Department of Obstetrics and Gynecology, Gunma University School of Medicine, Japan.
Miyamoto
K
K
Abe
Y
Y
Nakamura
T
T
Sugino
H
H
Eto
Y
Y
Shibai
H
H
Igarashi
M
M
D028341:Activins; D000818:Animals; D002454:Cell Differentiation; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005260:Female; D006107:Granulosa Cells / Q000187:drug effects / Q000378:metabolism*; D007265:Inhibins / Q000494:pharmacology*; D007328:Insulin / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D011962:Receptors, FSH / Q000096:biosynthesis* / Q000187:drug effects; D015291:Transforming Growth Factors / Q000494:pharmacology
D007328:Insulin; D011962:Receptors, FSH; D028341:Activins; D007265:Inhibins; D004815:Epidermal Growth Factor; D015291:Transforming Growth Factors