Active and passive immunization with Pseudomonas aeruginosa ribosomal vaccines and antisera in the burned rat model.
Pseudomonas aeruginosa ribosomal vaccines were tested for their ability to protect rats subjected to a 20% total body surface burn against the lethal effects of infection with homologous organisms. When administered prior to burning, the vaccines provided 100% protection. When administered postburning, the vaccine from one strain also provided 100% protection when the time interval between vaccination and infection was 3 days. When this time interval was reduced to 1 or 2 days, approximately 50% protection was obtained with the same vaccine. The vaccine from a second strain tested provided about 50% protection with a 3-day time interval. In addition, passive immunization using antiserum to a ribosomal vaccine was also demonstrated to be effective in protecting burned and infected rats, especially when multiple doses of antiserum were used. In this case, 80% protection was obtained (with no protection observed using multiple doses of normal serum). Finally, a comparison of ribosomal and lipopolysaccharide vaccines and antisera was also performed.
Lieberman
M M
MM
Walker
H L
HL
Ayala
E
E
Chapa
I
I
D000818:Animals; D001428:Bacterial Vaccines / Q000008:administration & dosage*; D002056:Burns / Q000150:complications*; D007106:Immune Sera / Q000008:administration & dosage; D007114:Immunization; D007116:Immunization, Passive; D011552:Pseudomonas Infections / Q000517:prevention & control*; D011550:Pseudomonas aeruginosa / Q000276:immunology*; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors; D014946:Wound Infection / Q000517:prevention & control*
D001428:Bacterial Vaccines; D007106:Immune Sera