Statistical positioning of nucleosomes by specific protein-binding to an upstream activating sequence in yeast.
Arrays of nucleosomes were positioned with respect to the GAL1-GAL10 intergenic region inserted into Saccharomyces cerevisiae minichromosomes. Deletions of DNA flanking the upstream activation sequence left the array unaltered, showing that nucleosome positioning was not a consequence of sequence-specific histone-DNA interactions but depended on proximity to the galactose-responsive upstream activation sequence (UASG). Replacement of the upstream activation sequence by synthetic oligonucleotides with different protein-binding properties identified a short sequence within this region that is responsible for the ordered array. This sequence overlaps a binding site for GAL4 protein, a positive regulator of transcription, but exerts its effect on chromatin structure independently of GAL4, probably through binding a novel factor that is not GAL-specific.
Fedor
M J
MJ
Department of Cell Biology, Stanford University School of Medicine, CA 94305.
Lue
N F
NF
Kornberg
R D
RD
D001483:Base Sequence; D001665:Binding Sites; D002843:Chromatin; D002875:Chromosomes; D004271:DNA, Fungal / Q000235:genetics; D005656:Fungal Proteins / Q000378:metabolism*; D009707:Nucleosomes / Q000378:metabolism*; D012045:Regulatory Sequences, Nucleic Acid; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism
D002843:Chromatin; D004271:DNA, Fungal; D005656:Fungal Proteins; D009707:Nucleosomes