title: A new revision of the sequence of plasmid pBR322.
abstract: A revised sequence in the region immediately upstream from the rop gene of pBR322 is reported. Two base pairs in the accepted sequence do not exist in the plasmid DNA. Specifically, a TA base pair is missing at sequence coordinate 1893 [Sutcliffe, Cold Spring Harbor Symp. Quant. Biol. 43 (1979) 77-90] and an AT base pair is missing at position 1915, giving a total size for pBR322 of 4361 bp. These changes are in a potential translation initiation sequence and probably reflect errors in the original sequence rather than recent evolution of the plasmid.
authors.affiliation: Molecular Biology Research, Upjohn Company, Kalamazoo, MI 49007.
authors.forename: N
authors.initials: N
authors.lastname: Watson
mesh_terms: D001482:Base Composition; D001483:Base Sequence; D004269:DNA, Bacterial; D004926:Escherichia coli / Q000235:genetics; D010957:Plasmids
chemical_list: D004269:DNA, Bacterial