Selective release of the Treponema pallidum outer membrane and associated polypeptides with Triton X-114.
The effects of the nonionic detergent Triton X-114 on the ultrastructure of Treponema pallidum subsp. pallidum are presented in this study. Treatment of Percoll-purified motile T. pallidum with a 1% concentration of Triton X-114 resulted in cell surface blebbing followed by lysis of blebs and a decrease in diameter from 0.25-0.35 micron to 0.1-0.15 micron. Examination of thin sections of untreated Percoll-purified T. pallidum showed integrity of outer and cytoplasmic membranes. In contrast, thin sections of Triton X-114-treated treponemes showed integrity of the cytoplasmic membrane but loss of the outer membrane. The cytoplasmic cylinders generated by detergent treatment retained their periplasmic flagella, as judged by electron microscopy and immunoblotting. Recently identified T. pallidum penicillin-binding proteins also remained associated with the cytoplasmic cylinders. Proteins released by Triton X-114 at 4 degrees C were divided into aqueous and hydrophobic phases after incubation at 37 degrees C. The hydrophobic phase had major polypeptide constituents of 57, 47, 38, 33-35, 23, 16, and 14 kilodaltons (kDa) which were reactive with syphilitic serum. The 47-kDa polypeptide was reactive with a monoclonal antibody which has been previously shown to identify a surface-associated T. pallidum antigen. The aqueous phase contained the 190-kDa ordered ring molecule, 4D, which has been associated with the surface of the organisms. Full release of the 47- and 190-kDa molecules was dependent on the presence of a reducing agent. These results indicate that 1% Triton X-114 selectively solubilizes the T. pallidum outer membrane and associated proteins of likely outer membrane location.
Cunningham
T M
TM
Department of Microbiology and Immunology, University of California, Los Angeles 90024.
Walker
E M
EM
Miller
J N
JN
Lovett
M A
MA
D001425:Bacterial Outer Membrane Proteins / Q000302:isolation & purification / Q000378:metabolism*; D001426:Bacterial Proteins; D002352:Carrier Proteins / Q000302:isolation & purification; D002462:Cell Membrane / Q000187:drug effects / Q000648:ultrastructure; D006602:Hexosyltransferases; D008854:Microscopy, Electron; D002267:Muramoylpentapeptide Carboxypeptidase / Q000302:isolation & purification; D017830:Octoxynol; D046915:Penicillin-Binding Proteins; D010458:Peptidyl Transferases; D011092:Polyethylene Glycols / Q000494:pharmacology*; D013089:Spermatocidal Agents / Q000494:pharmacology*; D014210:Treponema pallidum / Q000187:drug effects / Q000648:ultrastructure*
D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D002352:Carrier Proteins; D046915:Penicillin-Binding Proteins; D013089:Spermatocidal Agents; D011092:Polyethylene Glycols; D017830:Octoxynol; C010615:Nonidet P-40; D010458:Peptidyl Transferases; D006602:Hexosyltransferases; D002267:Muramoylpentapeptide Carboxypeptidase