title: Induction of antigen-specific proliferation in affinity-purified small B lymphocytes: requirement for BSF-1 by type 2 but not type 1 thymus-independent antigens.
abstract: We report here the role of B cell stimulatory factors in the induction of antigen-specific proliferation of affinity-purified small B lymphocytes. TI-1 antigens such as TNP-LPS and TNP-BA induced proliferation of hapten-binding B cells in the absence of exogenous B cell stimulatory factors. TI-2 antigens such as TNP-Ficoll required the co-stimulator BSF-1 to induce antigen-specific proliferation, and this response could be augmented by IL 1. TD antigens such as TNP-OVA were unable to induce antigen-specific proliferation either in the absence or presence of B cell stimulatory factors, and showed an absolute activation requirement for carrier-specific helper T cells. No role for IL 2 or BCGF II could be found in the factor-dependent proliferative response of hapten-binding B cells to TI-2 antigens, either as primary co-stimulators or as modulators of the response obtained with TNP-Ficoll, BSF-1, and IL 1. In contrast, concentrations of IFN-gamma that were nontoxic for normal B cells and B cell hybrids effectively abrogated the proliferative response of affinity-purified cells to TNP-Ficoll, BSF-1, and IL 1. By all of these criteria, the B cell activation requirements of TI-2 antigens appear to be identical to those previously published for soluble anti-IgM antibodies.
authors.forename: P
authors.initials: P
authors.lastname: Stein
authors.forename: P
authors.initials: P
authors.lastname: Dubois
authors.forename: D
authors.initials: D
authors.lastname: Greenblatt
authors.forename: M
authors.initials: M
authors.lastname: Howard
mesh_terms: D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology; D000955:Antigens, T-Independent / Q000145:classification / Q000276:immunology*; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology*; D002003:Brucella abortus / Q000276:immunology; D002469:Cell Separation / Q000379:methods; D005260:Female; D005362:Ficoll / Q000276:immunology; D006133:Growth Substances / Q000276:immunology*; D007075:Immunoglobulin M / Q000276:immunology; D007375:Interleukin-1 / Q000276:immunology; D015847:Interleukin-4; D008070:Lipopolysaccharides / Q000276:immunology; D008213:Lymphocyte Activation; D008222:Lymphokines / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000276:immunology; D014301:Trinitrobenzenes / Q000276:immunology
chemical_list: D000888:Antibodies, Anti-Idiotypic; D000955:Antigens, T-Independent; D006133:Growth Substances; D007075:Immunoglobulin M; D007375:Interleukin-1; D008070:Lipopolysaccharides; D008222:Lymphokines; D014301:Trinitrobenzenes; D015847:Interleukin-4; D005362:Ficoll