title: Characteristic features of thermal stability map of DNA in Escherichia coli and eukaryotic genes.
abstract: Distribution of double-helix thermal stability of Escherichia coli and eukaryotic DNAs was analyzed. The results confirmed the previous propositions based on the study of the stability distribution in phage DNAs: (1) stability fluctuation appears near the boundaries of protein coding regions (PCRs) and non protein coding regions (NPCRs); (2) PCRs have less fluctuation than NPCRs. The present analysis also revealed that the local G + C content is lower in the beginning of PCRs of E. coli than the average G + C content of PCR and that deviations in the amino acid composition and the third letter usage PCRs are involved in the low G + C content; the biological meaning of this is discussed in relation to mRNA structure.
authors.affiliation: Department of Physics, Faculty of Science, University of Tokyo, Japan.
authors.forename: R
authors.initials: R
authors.lastname: Hanai
authors.forename: A
authors.initials: A
authors.lastname: Suyama
authors.forename: A
authors.initials: A
authors.lastname: Wada
mesh_terms: D001482:Base Composition; D003062:Codon; D004247:DNA / Q000032:analysis / Q000235:genetics; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D005091:Exons; D005796:Genes; D005798:Genes, Bacterial; D007438:Introns; D009876:Operon; D012333:RNA, Messenger; D013696:Temperature; D013816:Thermodynamics
chemical_list: D003062:Codon; D004269:DNA, Bacterial; D012333:RNA, Messenger; D004247:DNA