The normal human H-ras1 gene can act as an onco-suppressor.
The altered morphology and tumorigenic phenotypes of rat 208F fibroblasts transformed with the human T24 H-ras1 oncogene is suppressed by transfection with the human normal H-ras1 gene. In the suppressed cells, both the normal and mutant T24 ras gene products are expressed although the normal p21 is expressed at a higher level. Rare transformants or tumours derived from suppressed cells possess reduced expression of normal ras p21. Our findings suggest that transforming ras alleles do not behave in a dominant manner and that elevated expression of the normal allele could cause suppression of the morphologically transformed and tumorigenic phenotypes.
Spandidos
A
A
Beatson Institute for Cancer Research, Bearsden, Glasgow, UK.
Wilkie
N M
NM
D000483:Alleles; D000818:Animals; D002471:Cell Transformation, Neoplastic / Q000235:genetics; D004247:DNA / Q000235:genetics; D005786:Gene Expression Regulation; D011905:Genes, ras; D006801:Humans; D009154:Mutation; D010641:Phenotype; D010957:Plasmids; D051381:Rats; D011995:Recombination, Genetic; D013489:Suppression, Genetic; D014162:Transfection
D004247:DNA