title: Monoclonals and allergy. A review, with experimental contribution.
abstract: The role of monoclonal antibodies in allergy has been explored. First the status of art of monoclonal research in general is reviewed, by outlining a monoclonals identikit and the relevant technologies employed for their development. The attention is then focused on the present impact of monoclonals in the allergological field, first considering a general outline, and then the important steps of standardization of monoclonal antibodies. A comprehensive hint is made concerning the monitoring of immunotherapy, with future extrapolations on developing anti-idiotype vaccines, of which same examples can already be found in the infectious field, thus leaving the way open in allergy as well. A second section deals with the experimental contribution of the Authors, with the description of the preparation of the allergenic extracts of D.F., with details of the relevant steps (rabbit immunization; extract characterization; techniques used for monoclonal screening and characterization). The results obtained are discussed in relation to the techniques employed, weighing the reciprocal advantages and drawbacks.
authors.affiliation: Istituto di Chimica e Propedeutica Biochimica, Università degli Studi di Brescia.
authors.forename: A
authors.initials: A
authors.lastname: Albertini
authors.forename: E
authors.initials: E
authors.lastname: Antoniazzi
authors.forename: S
authors.initials: S
authors.lastname: Archetti
authors.forename: G
authors.initials: G
authors.lastname: Bugari
authors.forename: R
authors.initials: R
authors.lastname: Cabula
authors.forename: E
authors.initials: E
authors.lastname: Ghezzi
authors.forename: S
authors.initials: S
authors.lastname: Ghielmi
authors.forename: G
authors.initials: G
authors.lastname: Sacchi
authors.forename: G C
authors.initials: GC
authors.lastname: Tassi
authors.forename: S
authors.initials: S
authors.lastname: Tombesi
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology / Q000628:therapy*; D007116:Immunization, Passive; D007158:Immunologic Techniques
chemical_list: D000911:Antibodies, Monoclonal