title: Specialized transduction of Pseudomonas aeruginosa PAO by bacteriophage D3.
abstract: D3, a temperate bacteriophage of Pseudomonas aeruginosa PAO, was found to specifically transduce the alleles met-49 and met-117. Induction of established lysogens with UV light was necessary for the production of transducing lysates. Transduced cells were immune to superinfection by phage D3 and could give rise to high-frequency transducing lysates. Cotransduction of these two alleles could not be demonstrated. met-117 was mapped to 26 min on the PAO genetic map. Complementation studies using the generalized transducing phage F116L indicated that met-49 is an allele of met-9011 which maps at 55 min. The integrated D3 prophage was shown to be coinherited with met-117 and with met-49.
authors.forename: M M
authors.initials: MM
authors.lastname: Cavenagh
authors.forename: R V
authors.initials: RV
authors.lastname: Miller
mesh_terms: D001435:Bacteriophages / Q000235:genetics*; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D005798:Genes, Bacterial; D008715:Methionine / Q000235:genetics; D011550:Pseudomonas aeruginosa / Q000235:genetics*; D011995:Recombination, Genetic; D014161:Transduction, Genetic
chemical_list: D008715:Methionine