[Demonstration of a cytosolic inhibitory of membrane estrone sulfatase activity in guinea pig liver].
Estrone sulfatase is a membrane-bound enzyme hydrolysing estrone sulfate. In normal and pathological tissues, estrone sulfatase is required for the conversion of estrone sulfate into physiologically active estrogens. In the hepatic cytosol of Guinea-pig, we have demonstrated the existence of a soluble inhibitor of uterine and hepatic microsomal estrone sulfatase. This inhibitor has approximatively a molecular weight of 7,600 and acts as a non-competitive inhibitor of estrone sulfatase. It seems to be a soluble intra-cellular effector of membrane-bound estrone sulfatase.
Adessi
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Moutaouakkil
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D000818:Animals; D002462:Cell Membrane / Q000201:enzymology; D003600:Cytosol / Q000032:analysis*; D005260:Female; D006168:Guinea Pigs; D007668:Kidney / Q000201:enzymology; D008099:Liver / Q000032:analysis*; D008861:Microsomes / Q000201:enzymology; D008862:Microsomes, Liver / Q000201:enzymology; D008970:Molecular Weight; D013429:Sulfatases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D014599:Uterus / Q000201:enzymology
D013429:Sulfatases; C020121:estrone sulfatase
Mise en évidence d'un inhibiteur cytosolique de l'activité estrone sulfatasique membranaire dans le foie de cobaye.