A yeast excision-repair gene is inducible by DNA damaging agents.
Plasmids containing various RAD-lacZ gene fusions were integrated into the chromosome of haploid yeast cells. These integrant strains were tested for expression of Escherichia coli beta-galactosidase after treatment with agents that damage DNA or interfere with normal DNA replication. We did not observe induction of single-copy RAD1-lacZ or RAD3-lacZ fusion genes under the experimental conditions used. However, exposure of cells containing an integrated RAD2-lacZ fusion gene to UV-radiation, gamma-radiation, 4-nitroquinoline 1-oxide, or nalidixic acid resulted in 4- to 6-fold enhanced expression of beta-galactosidase. Induction of the RAD2 gene after treatment of untransformed cells with 4-nitroquinoline 1-oxide was confirmed by direct examination of RAD2 mRNA. Lower levels of induction (approximately equal to 50%) were observed after treatment of cells with other chemicals. Induction of the RAD2-lacZ fusion gene was also observed in cells transformed with single-copy and multicopy autonomously replicating plasmids.
Robinson
G W
GW
Nicolet
C M
CM
Kalainov
D
D
Friedberg
E C
EC
D015112:4-Nitroquinoline-1-oxide / Q000494:pharmacology; D004260:DNA Repair; D004271:DNA, Fungal / Q000378:metabolism / Q000528:radiation effects; D004790:Enzyme Induction / Q000187:drug effects / Q000528:radiation effects; D005656:Fungal Proteins / Q000096:biosynthesis / Q000235:genetics*; D005720:Gamma Rays; D005800:Genes, Fungal; D009153:Mutagens / Q000494:pharmacology; D010957:Plasmids; D012331:RNA, Fungal / Q000032:analysis; D011994:Recombinant Proteins / Q000032:analysis; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics* / Q000528:radiation effects; D014158:Transcription, Genetic; D014466:Ultraviolet Rays; D001616:beta-Galactosidase / Q000032:analysis
D004271:DNA, Fungal; D005656:Fungal Proteins; D009153:Mutagens; D012331:RNA, Fungal; D011994:Recombinant Proteins; D015112:4-Nitroquinoline-1-oxide; D001616:beta-Galactosidase