Enhanced strength of endothelial attachment on polyester elastomer and polytetrafluoroethylene graft surfaces with fibronectin substrate.
Successful development of a vascular prosthesis lined with endothelium may depend on the ability of the attached cells to resist shear forces after implantation. The purpose of this article is to describe a model for measurement of endothelial detachment caused by shear stress and to identify biomaterials that resist loss of attached cells as a result of shear stress. With human umbilical venous endothelium labeled with indium 111-oxine, cellular attachment to uncoated and fibronectin-coated polyester elastomer and expanded polytetrafluoroethylene (e-PTFE) graft surfaces was quantified after an 18-hour incubation. PTFE grafts prepared by immediate seeding were also studied. The relative strength of endothelial attachment was determined by the percentage of the original inoculum remaining after the seeded graft surfaces were subjected to a physiologic shear stress of 15 dynes/cm2 during in vitro perfusion. In polyester elastomer grafts, fibronectin did not significantly increase initial attachment but did increase the percentage of inoculum remaining after perfusion (92.1% vs. 39.74%, p = 0.001). A similar relationship existed between fibronectin-coated e-PTFE and immediately seeded e-PTFE preparations with 61.6% and 25.8%, respectively, of the inoculum remaining after perfusion (p = 0.001). Furthermore, the percentage of inoculum retained on fibronectin-coated polyester elastomer was significantly greater than on fibronectin-coated e-PTFE (p = 0.001). In comparing uncoated grafts, polyester elastomer had 39.7% of the inoculum retained after perfusion whereas only 1.8% was remaining on the e-PTFE grafts (p = 0.0001). We conclude that polyester elastomer permits better endothelial cell attachment than e-PTFE and that fibronectin coating enhances the strength of attachment to both graft materials.
Kesler
K A
KA
Herring
M B
MB
Arnold
M P
MP
Glover
J L
JL
Park
H M
HM
Helmus
M N
MN
Bendick
P J
PJ
D001672:Biocompatible Materials; D001807:Blood Vessel Prosthesis; D004727:Endothelium / Q000502:physiology* / Q000648:ultrastructure; D005353:Fibronectins; D006801:Humans; D007204:Indium; D008855:Microscopy, Electron, Scanning; D008955:Models, Cardiovascular; D009942:Organometallic Compounds; D015125:Oxyquinoline / Q000031:analogs & derivatives; D011091:Polyesters; D011138:Polytetrafluoroethylene; D011474:Prosthesis Design; D011868:Radioisotopes; D013314:Stress, Mechanical; D013997:Time Factors
D001672:Biocompatible Materials; D005353:Fibronectins; D009942:Organometallic Compounds; D011091:Polyesters; D011868:Radioisotopes; D007204:Indium; C032950:indium oxine; D015125:Oxyquinoline; D011138:Polytetrafluoroethylene