title: Chemiluminescence of canine peripheral blood lymphocytes stimulated by mitogens.
abstract: Luminol-dependent chemiluminescence of peripheral blood lymphocytes from dogs stimulated with concanavalin A (Con A) or phytohemagglutinin P (PHA) was measured with a Pico-Lite luminometer. 10 microliter of luminol gave optimal quantum yield from 1 X 10(6) lymphocytes sensitized with either 80 micrograms Con A or 160 micrograms PHA. Addition of superoxide dismutase did not influence the course of chemiluminescence. Whereas catalase produced 41% increase in quantum yield, mannitol caused a 51% inhibition of chemiluminescence. Lymphocytes exposed to varying doses of short term x-irradiation or lymphocytes isolated from dogs kept under continuous exposure through a gamma irradiation source showed dose-related depression of chemiluminescence. Membrane factors may be involved in lymphocyte stimulation to chemiluminescence as pulse experiments with Con A and PHA revealed. It is proposed that chemiluminescence measurements may be useful in monitoring early events in lymphocyte stimulation by antigens and mitogens.
authors.forename: G O
authors.initials: GO
authors.lastname: Ekejindu
authors.forename: M
authors.initials: M
authors.lastname: Shifrine
authors.forename: H P
authors.initials: HP
authors.lastname: Misra
mesh_terms: D000818:Animals; D002374:Catalase / Q000494:pharmacology; D003208:Concanavalin A / Q000494:pharmacology; D004285:Dogs; D005720:Gamma Rays; D008163:Luminescent Measurements; D008165:Luminol / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000187:drug effects / Q000528:radiation effects; D008353:Mannitol / Q000494:pharmacology; D010777:Photochemistry; D010835:Phytohemagglutinins / Q000494:pharmacology; D011724:Pyridazines / Q000494:pharmacology*; D011975:Receptors, Mitogen / Q000032:analysis; D013482:Superoxide Dismutase / Q000494:pharmacology
chemical_list: D010835:Phytohemagglutinins; D011724:Pyridazines; D011975:Receptors, Mitogen; D003208:Concanavalin A; D008353:Mannitol; D008165:Luminol; D002374:Catalase; D013482:Superoxide Dismutase