title: Molecular cloning and sequence analysis of a developmentally regulated cysteine-rich outer membrane protein from Chlamydia trachomatis.
abstract: Two overlapping genomic fragments have been cloned from Chlamydia trachomatis serovar L1 DNA. Sequence determination of 2530 bp has revealed two open reading frames coding for 'cysteine-rich' (Cr) proteins. One of these proteins was confirmed, by analysis of the inferred amino acid sequence, as the 60-kDa Cr outer membrane protein associated with differentiation of reticulate bodies (RBs) into elementary bodies (EBs). The other smaller 15-kDa protein contained a high percentage of methionine and cysteine and may correspond to a reported smaller and co-ordinately synthesised Cr outer-membrane protein also associated with RB to EB differentiation. Sequencing showed three potential stem-loop structures within the 5', 3' and intergenic regions of the cloned fragment. Southern-blot analysis revealed that the cloned fragment is conserved in ten serovars of C. trachomatis and that a strongly cross-hybridising fragment is also present in Chlamydia psittaci.
authors.affiliation: Department of Microbiology, University Medical School, Southampton General Hospital, U.K.
authors.forename: I N
authors.initials: IN
authors.lastname: Clarke
authors.forename: M E
authors.initials: ME
authors.lastname: Ward
authors.forename: P R
authors.initials: PR
authors.lastname: Lambden
mesh_terms: D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D001483:Base Sequence; D002692:Chlamydia trachomatis / Q000235:genetics*; D003001:Cloning, Molecular; D003545:Cysteine / Q000032:analysis*; D004247:DNA / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization
chemical_list: D001425:Bacterial Outer Membrane Proteins; D004247:DNA; D003545:Cysteine