title: Multiple aflatoxin B1 binding proteins exist in rat liver cytosol.
abstract: The in vitro binding of aflatoxin B1 to rat liver cytosolic proteins was investigated. Aflatoxin B1 binding activity was assayed with protein purified by gel permeation chromatography, ammonium sulfate fractionation, and DEAE-cellulose chromatography. Twenty-five percent of the total binding activity was associated with proteins eluted by 0 and 0.1 M NaCl. Over 50% of the total binding activity was associated with protein present in the 0.2 M NaCl fraction. Glutathione S-transferase activity was also monitored and found only in the low salt (less than 0.2 M NaCl) fractions. The proteins eluted by 0.2 M NaCl were further purified by hydroxylapatite column chromatography and binding was found predominantly in a single fraction. The protein purification steps resulted in a 20-fold increase in the specific binding activity over that initially observed in the cytosol. These results indicate that multiple proteins are capable of binding aflatoxin B1 in rat liver cytosol.
authors.forename: P
authors.initials: P
authors.lastname: Taggart
authors.forename: T M
authors.initials: TM
authors.lastname: Devlin
authors.forename: J J
authors.initials: JJ
authors.lastname: Ch'ih
mesh_terms: D016604:Aflatoxin B1; D000348:Aflatoxins / Q000378:metabolism*; D000645:Ammonium Sulfate; D000818:Animals; D002352:Carrier Proteins / Q000302:isolation & purification / Q000378:metabolism; D005591:Chemical Fractionation; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D003593:Cytoplasm / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D008099:Liver / Q000201:enzymology / Q000378:metabolism*; D051381:Rats
chemical_list: D000348:Aflatoxins; D002352:Carrier Proteins; D016604:Aflatoxin B1; D005982:Glutathione Transferase; D000645:Ammonium Sulfate