Effects of 2-methylthio ATP on insulin secretion in the dog in vivo.
The effects of 2-methylthio ATP, an ATP analogue that is more specific for the P2Y receptor, were investigated on insulin secretion in the anesthetized dog in vivo. 2-Methylthio ATP was infused directly into the pancreaticoduodenal artery for 15 min. The infusion was performed so as to obtain a pancreaticoduodenal artery blood level of about 15 microM. 2-Methylthio ATP induced an immediate and significant stimulation of insulin secretion measured from the pancreaticoduodenal vein by means of a T-shaped catheter. After the infusion was stopped, the secretion of insulin progressively decreased and at 30 min was close to basal values. The stimulation of insulin secretion induced a transient but significant reduction of peripheral venous blood glucose levels.
Ribes
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G
Faculté de Médecine, Unité Associée au CNRS No. 599, Institut de Biologie, Montpellier, France.
Bertrand
G
G
Petit
P
P
Loubatières-Mariani
M M
MM
D000255:Adenosine Triphosphate / Q000031:analogs & derivatives* / Q000494:pharmacology; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D001794:Blood Pressure / Q000187:drug effects; D004285:Dogs; D004386:Duodenum / Q000098:blood supply; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D010179:Pancreas / Q000098:blood supply; D012039:Regional Blood Flow / Q000187:drug effects; D013873:Thionucleotides / Q000494:pharmacology*
D001786:Blood Glucose; D007328:Insulin; D013873:Thionucleotides; D000255:Adenosine Triphosphate; C008501:2-methylthio-ATP