A new method of non-enzymatic dissociation of the Bufo retina.
We have formulated a medium in which an electroretinogram (ERG) can be elicited from eyecup pieces of Bufo marinus up to 2 weeks after dissection. At the same time the retina can be dissociated into healthy, intact photoreceptors and other cells after soaking in the same medium from 4 to 24 h. Thus, no enzymes need be used which have deleterious effects on the cells; on the contrary the medium extends cell viability. Clearly, this method should be attractive in electrophysiological and biochemical studies.
Leibovic
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D000818:Animals; D002024:Bufo marinus; D002469:Cell Separation / Q000379:methods*; D002470:Cell Survival; D002478:Cells, Cultured; D003470:Culture Media; D004594:Electrophysiology; D006863:Hydrogen-Ion Concentration; D010447:Peptide Hydrolases; D012160:Retina / Q000166:cytology*; D013997:Time Factors
D003470:Culture Media; D010447:Peptide Hydrolases