[A rapid and simple method for extracting intracellular metabolites from Escherichia coli bacteria].
The article deals with the development of a new method for the extraction of intracellular glycolytic metabolites from bacterial cells. The study has been made on the culture of E. coli B/r CSH. In accordance with this method, the same bacterial filter is used for both filtration (the removal of the culture fluid) and the extraction of low-molecular components of the cells with perchloric acid. The advantage of this method is the absence of unnecessary operations due to the use of a filter installation designed by the author. Quantitatively, this method yields better and reproducible results. The filtration capacity of different types of filters has been analyzed. The optimal time for the extraction of low-molecular cell components has been determined. A change in the concentration of pyruvate in the process of the cellular cycle of E. coli synchronous culture grown in the presence of glucose has been shown to occur. The newly developed method of extraction can be used not only for E. coli, but also for cells of other types.
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D001431:Bacteriological Techniques / Q000295:instrumentation; D002458:Cell Fractionation / Q000295:instrumentation / Q000379:methods*; D004926:Escherichia coli / Q000302:isolation & purification / Q000378:metabolism*; D005285:Fermentation; D005374:Filtration / Q000295:instrumentation; D013997:Time Factors
Bystryĭ i prostoĭ sposob ékstraktsii vnutrikletochnykh metabolitov iz bakteriĭ Escherichia coli.