Detection of the cytogenetic effect of inhaled aerosols by the micronucleus test.
The induction of micronuclei in mice exposed to aerosols of the following 6 genotoxic chemicals by inhalation was examined: cyclophosphamide (CP), methyl methanesulfonate (MMS), mitomycin C (MMC), dimethylnitrosamine (DMN), ethylcarbamate and colchicine. Exposure of mice to CP aerosols at a theoretical concentration of 2426 mg/m3 for 29, 81 and 139 min induced 0.6, 1.0 and 2.3% micronucleated polychromatic erythrocytes (MNPCEs) in bone marrow 24 h after the termination of exposure. The other chemicals except for DMN showed a similar exposure-response relationship following in vivo exposures to their aerosols. The results obtained in this study suggest that the cytogenetic effect of inhaled aerosols can be detected by the micronucleus test, and the method described in the present report is useful as a rapid in vivo test for atmospheric aerosols.
Odagiri
Y
Y
Adachi
S
S
Katayama
H
H
Takemoto
K
K
D000336:Aerosols / Q000009:adverse effects*; D000818:Animals; D001853:Bone Marrow / Q000187:drug effects; D003078:Colchicine / Q000009:adverse effects; D003520:Cyclophosphamide / Q000009:adverse effects; D004128:Dimethylnitrosamine / Q000009:adverse effects; D005260:Female; D008741:Methyl Methanesulfonate / Q000009:adverse effects; D051379:Mice; D016685:Mitomycin; D008937:Mitomycins / Q000009:adverse effects; D009152:Mutagenicity Tests / Q000379:methods*; D009153:Mutagens; D014520:Urethane / Q000009:adverse effects
D000336:Aerosols; D008937:Mitomycins; D009153:Mutagens; D014520:Urethane; D016685:Mitomycin; D003520:Cyclophosphamide; D008741:Methyl Methanesulfonate; D004128:Dimethylnitrosamine; D003078:Colchicine