Effect of progesterone in long-term endometrial cell culture: study of the synthesis of prostaglandins, thromboxane, prostacyclin and prolactin.
Cultured monolayers of endometrial cells synthesized PGE2, PGF2 alpha, 13,14-dihydro-15-keto-PGF2 alpha (DHKF2 alpha), TXB2 and 6-keto-PGF1 alpha. Synthesis increased with the duration of culture, reaching a maximum at approximately the 20th day for PGF2 alpha and PGE2; PGF2 alpha levels always exceeded those of PGE2. Transformation of PGF2 alpha into DHKF2 alpha was minimal. Maximal levels of TXB2 and 6-keto-PGF1 alpha were noted after 7 and 14 days respectively. After three weeks, traces of DHKF2 alpha, TXB2 and 6-keto-PGF1 alpha were noted, whereas PGF2 alpha levels were marked after 30 days of culture. Addition of progesterone (0.32 ng/ml) reduced PG production, notably for PGF2 alpha, but promoted prolactin synthesis, indicating that this progesterone concentration induced decidualization of endometrial cells during the first days of culture.
Nemme
R
R
INSERM Unité 187, Clamart, France.
Acker
G M
GM
Papiernik
E
E
D015121:6-Ketoprostaglandin F1 alpha / Q000096:biosynthesis; D002478:Cells, Cultured; D015237:Dinoprost / Q000031:analogs & derivatives / Q000096:biosynthesis; D015232:Dinoprostone / Q000096:biosynthesis; D004717:Endometrium / Q000166:cytology / Q000187:drug effects*; D011464:Epoprostenol / Q000096:biosynthesis; D005260:Female; D006801:Humans; D011374:Progesterone / Q000494:pharmacology*; D011388:Prolactin / Q000096:biosynthesis*; D011453:Prostaglandins / Q000096:biosynthesis*; D013929:Thromboxane B2 / Q000096:biosynthesis
D011453:Prostaglandins; D011374:Progesterone; D013929:Thromboxane B2; D015121:6-Ketoprostaglandin F1 alpha; C051536:13,14-dihydroxy-15-ketoprostaglandin F2alpha; D011388:Prolactin; D015237:Dinoprost; D011464:Epoprostenol; D015232:Dinoprostone