title: GAL2 codes for a membrane-bound subunit of the galactose permease in Saccharomyces cerevisiae.
abstract: The gene encoding the galactose permease of Saccharomyces cerevisiae (GAL2) was cloned. The clone restores galactose permease activity to gal2 yeasts and is regulated by galactose in a manner similar to other GAL gene products (GAL1, -7, and -10). Experiments with temperature-conditional secretory mutants indicated that transport of the GAL2 gene product to the cell surface requires a functional secretory pathway. In addition, gene fusions were constructed between the GAL2 gene and the Escherichia coli lacZ gene. The GAL2-lacZ gene fusions code for galactose-regulated beta-galactosidase activity in yeasts. The beta-galactosidase activity was found to be membrane bound.
authors.forename: J F
authors.initials: JF
authors.lastname: Tschopp
authors.forename: S D
authors.initials: SD
authors.lastname: Emr
authors.forename: C
authors.initials: C
authors.lastname: Field
authors.forename: R
authors.initials: R
authors.lastname: Schekman
mesh_terms: D001692:Biological Transport; D002462:Cell Membrane / Q000201:enzymology; D005796:Genes; D005800:Genes, Fungal; D005818:Genetic Engineering; D026901:Membrane Transport Proteins / Q000096:biosynthesis / Q000235:genetics*; D009004:Monosaccharide Transport Proteins; D009154:Mutation; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics; D001616:beta-Galactosidase / Q000032:analysis / Q000235:genetics
chemical_list: D026901:Membrane Transport Proteins; D009004:Monosaccharide Transport Proteins; C043910:galactose permease; D001616:beta-Galactosidase