title: Secretion in yeast of human lysozymes with different specific activities created by replacing valine-110 with proline by site-directed mutagenesis.
abstract: Computer graphics indicate that a steric hindrance exists between valine-110 side chain of human lysozyme (EC 3.2.1.17) and an acetyl group of a modified substrate that contains N6,O-diacetylmuramic acid. To alter the substrate specificity of human lysozyme to be effective on the modified substrate, we replaced the valine-110 residue with various amino acids by site-directed mutagenesis. One of the mutant proteins (valine residue replaced with proline:P110) was secreted in Saccharomyces cerevisiae as at least four components (P110-A, P110-B, P110-C, and P110-D) with different specific activities. Two components, P110-B and P110-D, were isolated in a pure form and structurally characterized. The results suggest that this mutation lowered the lytic activity against Micrococcus lysodeikticus by changing a local conformation of the catalytic site while keeping almost the same substrate binding sites. Our results also indicate that cis/trans isomerization of prolyl peptide bonds probably occurs in vivo and that the conformational change of protein as well as point mutations in genes might influence the molecular evolution of the protein.
authors.affiliation: Protein Engineering Research Institute, Osaka, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Kikuchi
authors.forename: Y
authors.initials: Y
authors.lastname: Yamamoto
authors.forename: Y
authors.initials: Y
authors.lastname: Taniyama
authors.forename: K
authors.initials: K
authors.lastname: Ishimaru
authors.forename: W
authors.initials: W
authors.lastname: Yoshikawa
authors.forename: Y
authors.initials: Y
authors.lastname: Kaisho
authors.forename: M
authors.initials: M
authors.lastname: Ikehara
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D002942:Circular Dichroism; D003196:Computer Graphics; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D009113:Muramidase / Q000235:genetics*; D009154:Mutation; D011392:Proline; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D013379:Substrate Specificity; D014633:Valine
chemical_list: D000596:Amino Acids; D011392:Proline; D009113:Muramidase; D014633:Valine