Neutralization of lymphokine-mediated antirickettsial activity of fibroblasts and macrophages with monoclonal antibody specific for murine interferon gamma.
Lymphokine-mediated inhibition of Rickettsia prowazekii multiplication in L929 fibroblasts was eliminated by treatment of the lymphokine with a monoclonal antibody specific for interferon-gamma. Soluble monoclonal antibody and antibody conjugated to Sepharose beads were equally effective. Macrophage activation to limit the multiplication of Rickettsia conorii was eliminated with antibody-conjugated beads; however, neutralization of the ability to activate macrophages with soluble antibody was not complete and required more antibody than did neutralization of antiviral activity.
Jerrells
T R
TR
Turco
J
J
Winkler
H H
HH
Spitalny
G L
GL
D000818:Animals; D000911:Antibodies, Monoclonal; D002478:Cells, Cultured; D005347:Fibroblasts / Q000276:immunology*; D007371:Interferon-gamma / Q000276:immunology*; D008264:Macrophages / Q000276:immunology*; D008297:Male; D051379:Mice; D012283:Rickettsia prowazekii / Q000254:growth & development* / Q000276:immunology
D000911:Antibodies, Monoclonal; D007371:Interferon-gamma