Glucosidation of estradiol-17 beta in the cultured ovaries of the silkworm, Bombyx mori.
Metabolic pathways of estradiol-17 beta and other vertebrate steroid hormones of cultured silkworm pupal ovaries were examined using 14C-labeled steroids. The isolated ovaries showed significant uptake and metabolic activity of the 14C-labeled estradiol-17 beta added to the medium. Analysis of the metabolized compounds by thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC) revealed extensive metabolic conversion of [14C]estradiol-17 beta and estrone; i.e., estrone was reduced to estradiol-17 beta and estradiol-17 beta was metabolized to conjugates, including estradiol-3-beta-D-glucoside and estradiol-17-alpha-D-glucoside. [14C]Testosterone was not transformed appreciably by the ovaries. Metabolic activity and physiological significance of the vertebrate steroid hormones in the silkworm ovaries are discussed.
Ogiso
M
M
Fujimoto
Y
Y
Ikekawa
N
N
Ohnishi
E
E
D000818:Animals; D012831:Bombyx / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D004958:Estradiol / Q000378:metabolism*; D004970:Estrone / Q000378:metabolism; D005216:Fat Body / Q000378:metabolism; D005260:Female; D006868:Hydrolysis; D008297:Male; D009924:Organ Culture Techniques; D010053:Ovary / Q000378:metabolism; D011679:Pupa / Q000378:metabolism; D013737:Testis / Q000378:metabolism; D013739:Testosterone / Q000378:metabolism; D000520:alpha-Glucosidases; D001617:beta-Glucosidase
D004970:Estrone; D013739:Testosterone; D004958:Estradiol; D000520:alpha-Glucosidases; D001617:beta-Glucosidase