[The safety of a vaccinal preparation made of bacteria of the genus Proteus: the absence of a mutagenic effect].
The protein-lipopolysaccharide vaccinal preparation, obtained by the disintegration of microorganisms of the genus Proteus by means of hydrochloric hydroxylamine, has been tested for mutagenicity. Two tests have been used: (1) the count of reversion to histamine-dependence in Salmonella typhimurium cells, strains TA-98 and TA-100, intended for detecting mutations, such as read-through frame shift and, respectively, the change of nitrous bases in DNA; and (2) the count of structural aberrations in chromosomes and numeric karyotype changes on metaphase plates from the marrow of mice. In some of the tests the vaccine has shown mutagenic properties.
Volgareva
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Tolcheev
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D000818:Animals; D001428:Bacterial Vaccines / Q000633:toxicity*; D001711:Biotransformation / Q000187:drug effects; D001853:Bone Marrow / Q000187:drug effects; D002869:Chromosome Aberrations; D008099:Liver / Q000187:drug effects; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA; D009152:Mutagenicity Tests / Q000379:methods; D009153:Mutagens; D011514:Proteus vulgaris / Q000276:immunology*; D051381:Rats; D011919:Rats, Inbred Strains; D012486:Salmonella typhimurium / Q000187:drug effects; D013997:Time Factors
D001428:Bacterial Vaccines; D009153:Mutagens
Izuchenie bezvrednosti vaktsinnogo preparata iz bakteriĭ roda Proteus: otsutstvie mutagennogo éffekta.