Influence of recombinant human granulocyte-macrophage colony-stimulating factor on granulocyte functions.
Besides its effect on bone marrow progenitors, GM-CSF is able to interact with mature cells such as granulocytes which express GM-CSF receptors at their surface. Using an incubation period of 90 min, which corresponds to the maximum expression of GM-CSF receptors, we studied oxidative metabolism, random migration and chemotaxis as well as elastase release. Elastase was chosen as a marker for primary granules. We observed inhibition of chemotaxis and random migration, and stimulation of oxidative metabolism as well as elastase release in presence of rh GM-CSF. These different effects were dependent on the dosage of rh GM-CSF applied.
Klausmann
M
M
Dept. of Medicine, Univ. of Marburg, W. Germany.
Pflüger
K H
KH
Krumwieh
D
D
Seiler
F R
FR
Havemann
K
K
D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D003115:Colony-Stimulating Factors / Q000502:physiology*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D006098:Granulocytes / Q000187:drug effects / Q000502:physiology*; D006133:Growth Substances / Q000502:physiology*; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D008163:Luminescent Measurements; D010101:Oxygen Consumption / Q000187:drug effects; D010196:Pancreatic Elastase / Q000097:blood; D011994:Recombinant Proteins / Q000494:pharmacology*; D012016:Reference Values
D003115:Colony-Stimulating Factors; D006133:Growth Substances; D011994:Recombinant Proteins; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D010196:Pancreatic Elastase