title: A temporally expressed gene from Schwanniomyces alluvius and detection of homologous sequences in other yeasts.
abstract: The boundaries of a temporally expressed gene (EG1) located on a 6.5-kb HindIII chromosomal DNA fragment of the yeast Schwanniomyces alluvius have been defined by cDNA hybridization and S1 nuclease mapping. The sequence of this gene reveals a 474-bp open reading frame (158 aa) and a TATA-like sequence 100 bp upstream from the first start codon. The encoded protein bears little resemblance with other known yeast structural proteins, including histones. A portion of the 3' untranslated region shows 50% identity with a consensus repetitive sequence (B2) found in the mouse genome. Repetitive sequences are also located in both 5' and 3' untranslated regions. Genomic DNA analysis indicated that related sequences were detected at two loci in Schw. alluvius and one locus in Saccharomyces cerevisiae. In contrast to the continuous transcription pattern of the ADE1 genes of Schw. alluvius and S. cerevisiae, the 0.8-kb transcripts of EG1 in these yeasts were most abundant in cells in early-log phase of growth and absent in cells of late-log phase, suggesting discontinuous synthesis.
authors.affiliation: Molecular Genetics Section, National Research Council of Canada, Ottawa, Ontario.
authors.forename: K
authors.initials: K
authors.lastname: Prakash
authors.forename: V L
authors.initials: VL
authors.lastname: Seligy
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000235:genetics / Q000302:isolation & purification; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D012331:RNA, Fungal / Q000235:genetics / Q000302:isolation & purification; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics; D004718:Saccharomycetales / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D014158:Transcription, Genetic
chemical_list: D004271:DNA, Fungal; D012331:RNA, Fungal