Enzymatic characterization of three aeromonas species using API Peptidase, API "Osidase," and API Esterase test kits.
An enzymatic characterization of 16 strains of Aeromonas species including A. hydrophila (7), A. sobria (5), and A. caviae (4) was carried out using API Peptidase (strips numbered 1, 2, 3, 4, 5, and 6); API Esterase and API "Osidase" test strips. A total of 89 substrates was used in the assay and included 59 arylamides (aminopeptides), 10 esters, and 20 carbohydrates. All three species were remarkably uniform in their reactivities. Nineteen (32%) of the arylamide substrates used were hydrolyzed by all three species. Very strong arylamidase activity was displayed by all three species for L-lysine, L-hydroxyproline, L-arginine, L-alanine, L-proline, and L-leucyl-L-alanine. Esterase activity was strongest against caproate (C6), caprylate (C8), nonanoate (C9), and caprate (C10) substrates. Only a limited number of carbohydrate substrates were hydrolyzed; strong N-acetyl-beta-D-glucosaminidase activity was given by all strains. Both A. hydrophila and A. caviae gave strong beta-D-glucosidase reactivities, while A. sobria appeared to be negative for this enzyme. The results of our preliminary study show that some of the enzymes examined may be useful in the identification and differentiation of these species. The API enzyme assays yielded rapid (4 hr) results. The assays were easy to perform, relatively inexpensive and reproducible. The importance of replicate testing and the inclusion of uninoculated (buffer only) controls as part of the assay is emphasized.
Carnahan
A M
AM
Department of Microbiology, University of Maryland, College Park 20742.
O'Brien
M
M
Joseph
S W
SW
Colwell
R R
RR
D000333:Aeromonas / Q000145:classification* / Q000201:enzymology; D004950:Esterases / Q000032:analysis*; D010088:Oxidoreductases / Q000032:analysis*; D010447:Peptide Hydrolases / Q000032:analysis*; D011933:Reagent Kits, Diagnostic
D011933:Reagent Kits, Diagnostic; D010088:Oxidoreductases; D004950:Esterases; D010447:Peptide Hydrolases