title: Multiple uses of liquepipets in Southern, northern, and western blots.
abstract: Individually wrapped, sterile disposable transfer pipets can be used in the isolation of ds-DNA and ds-RNA fragments from gels as well as in the screening of multiple samples in Southern, Northern, and Western blots without potential contamination by exogenous nucleases and proteases. The sensitivity and results obtained by this method are comparable to those obtained by conventional methods. All the prehybridization, blocking, hybridization, and detection processes can be performed within the transfer pipet. The isotopically labeled probes used in hybridization can easily be recovered, stored for reuse, or disposed of as waste with no potential contamination of personnel or laboratory equipment. Strip blots are stable in appropriate buffers within the liquepipets which can be shipped easily worldwide for comparative analyses by collaborative investigators. This method is simple, time saving, and inexpensive and is particularly suitable for multiple sample screening. Other potential applications of this procedure are discussed.
authors.affiliation: Department of Biology, Utah State University, Logan 84322-5500.
authors.forename: J K
authors.initials: JK
authors.lastname: Li
authors.forename: T M
authors.initials: TM
authors.lastname: Johnson
authors.forename: B D
authors.initials: BD
authors.lastname: Parker
mesh_terms: D015152:Blotting, Northern / Q000295:instrumentation*; D015139:Blotting, Southern / Q000295:instrumentation*; D015153:Blotting, Western / Q000295:instrumentation*; D004279:DNA, Viral / Q000302:isolation & purification; D004209:Disposable Equipment / Q000706:statistics & numerical data*; D015336:Molecular Probe Techniques / Q000295:instrumentation*; D012330:RNA, Double-Stranded / Q000302:isolation & purification
chemical_list: D004279:DNA, Viral; D012330:RNA, Double-Stranded