[Continuous production of anti-hepatitis B surface antigen monoclonal antibody in hollow fiber perfusion bioreactor].
Hybridoma cell line H440Ac was cultivated continuously in hollow fiber perfusion bioreactor for fifty days to produce anti-hepatitis B surface antigen (anti-HBsAg) monoclonal antibody. A total of 90 mg anti-HBsAg antibody was produced in the hollow fiber bioreactor with 1000 cm2 membrane surface area. The collected production medium has an average antibody concentration of 0.45 mg/ml with a high concentration of 2.3 mg/ml. This compares with 0.016 and 1.1 mg/ml antibody concentration as collected from spinner flask and mouse ascites. The purity of the produced antibody is 0.085, 0.0028 and 0.026 mg antibody per mg of total harvested protein in the culture media produced from the hollow fiber bioreactor, spinner flasks and mouse ascites, respectively.
Meng
M H
MH
Development Center for Biotechnology, Taipei, Taiwan, ROC.
Chen
G C
GC
Hsieh
J H
JH
Yueh
Y
Y
Chen
S C
SC
Chang
T H
TH
Tsao
D
D
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D002452:Cell Count; D002460:Cell Line; D002470:Cell Survival; D002478:Cells, Cultured; D003470:Culture Media; D003584:Cytological Techniques / Q000295:instrumentation; D004867:Equipment Design; D006514:Hepatitis B Surface Antigens / Q000276:immunology*; D006825:Hybridomas / Q000166:cytology / Q000378:metabolism; D007074:Immunoglobulin G / Q000096:biosynthesis; D051379:Mice; D008807:Mice, Inbred BALB C; D010477:Perfusion
D000911:Antibodies, Monoclonal; D003470:Culture Media; D006514:Hepatitis B Surface Antigens; D007074:Immunoglobulin G