title: Mapping of rRNA genes with integrable plasmids in Bacillus subtilis.
abstract: Integrable plasmids pGR102 and pWR103 containing ribosomal sequences from within the transcriptional units for 16S and 23S were used to transform Bacillus subtilis. To date, these plasmids integrated into 7 of 10 known rrn operons. Two such events occurred at unassigned operons, revealing a close linkage of the CAT gene of the plasmid to pha-1 situated between dal-1 and purB33 for rrnE and to thiA78 situated between glyB133 and re-12 for rrnD. All seven integration events that led to the loss of unique ribosomal BclI fragments can now be assigned to known rrn operons.
authors.forename: G
authors.initials: G
authors.lastname: LaFauci
authors.forename: R L
authors.initials: RL
authors.lastname: Widom
authors.forename: R L
authors.initials: RL
authors.lastname: Eisner
authors.forename: E D
authors.initials: ED
authors.lastname: Jarvis
authors.forename: R
authors.initials: R
authors.lastname: Rudner
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics*; D001483:Base Sequence; D002874:Chromosome Mapping; D005798:Genes, Bacterial; D009693:Nucleic Acid Hybridization; D009876:Operon; D010957:Plasmids; D012335:RNA, Ribosomal / Q000235:genetics*; D014169:Transformation, Bacterial
chemical_list: D012335:RNA, Ribosomal