The retinal pigment epithelium and other tissues immunocytochemical studies using hybridoma supernatants.
We describe the production of hybridomas producing antibody reactive with retinal pigment epithelium. The patterns of reactivity obtained when supernatants from these hybrids were used to immuno-stain ocular and non-ocular tissues are described. From the patterns of reactivity obtained we suggest that the RPE shares common features with simple and glandular epithelia as well as myoepithelial cells. These findings may lend support to the hypotheses of RPE cell migration and contraction in the formation of epiretinal membranes and retinal detachment.
McKechnie
N M
NM
Institute of Ophthalmology, London, UK.
Alexander
R A
RA
Sura
G S
GS
Grierson
I
I
D000818:Animals; D000906:Antibodies / Q000032:analysis; D002415:Cats; D002417:Cattle; D005455:Fluorescent Antibody Technique; D006825:Hybridomas / Q000276:immunology*; D007124:Immunoenzyme Techniques; D010857:Pigment Epithelium of Eye / Q000276:immunology*
D000906:Antibodies