A simplified serological test for leprosy based on a 3,6-di-O-methylglucose-containing synthetic antigen.
The recent advent of synthetic antigens containing the Mycobacterium leprae-specific epitope, 3,6-di-O-methyl-beta-D-glucopyranoside, has allowed the development of simple specific serological tests for leprosy. The incorporation of one such product, 8-carbonyloctyl O-[4-O-(3,6-di-O-methyl-beta-D-glucopyranosyl)-alpha-L- rhamnopyranoside]-BSA, into a simple "spot" test, diffusion-in-gel enzyme-linked immunosorbent assay (ELISA), allowed an over 90% detection rate of untreated lepromatous leprosy, and the results showed good concordance with conventional ELISA based on the native phenolic glycolipid I.
Cho
S N
SN
Chatterjee
D
D
Brennan
P J
PJ
D000942:Antigens, Bacterial / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006017:Glycolipids / Q000276:immunology; D006023:Glycoproteins / Q000138:chemical synthesis / Q000276:immunology*; D006801:Humans; D005779:Immunodiffusion; D007918:Leprosy / Q000175:diagnosis*; D008757:Methylglucosides / Q000276:immunology*; D008759:Methylglycosides / Q000276:immunology*; D009166:Mycobacterium leprae / Q000276:immunology*; D012698:Serologic Tests
D000942:Antigens, Bacterial; D006017:Glycolipids; D006023:Glycoproteins; D008757:Methylglucosides; D008759:Methylglycosides