Molecular and functional properties of protein SS1 from small ribosomal subunits of Streptomyces aureofaciens.
Small ribosomal subunits of gram-positive cells of Streptomyces aureofaciens contain an acidic protein designated SS1. Purified protein SS1 has the same mobility in sodium dodecyl sulfate/polyacrylamide gel as ribosomal protein S1 of Escherichia coli (apparent Mr 68 000). Protein SS1 was dissected under mild conditions with trypsin and generated fragments were compared with well-characterized fragments of protein S1. The protein SS1 contains a structure homologous with the C-terminal fragment of protein S1. The affinity of protein SS1 to poly(U) is virtually identical with that of E. coli protein S1. In contrast to protein S1, the addition of SS1 to partially S1-depleted ribosomes of E. coli had no stimulatory effect on poly(U)-directed synthesis of polyphenylalanine. At molar excess of SS1 over ribosomes, the protein had comparable inhibitory effect on polypeptide synthesis as had S1 of E. coli. Ribosomes of S. aureofaciens required about one order of magnitude higher concentration of poly(U) for maximum synthetic activity than did ribosomes of E. coli. The addition of proteins SS1 or S1 to ribosomes of S. aureofaciens had no stimulatory effect on translation of poly(U). Our data indicate that the high-molecular-mass acidic protein SS1 of small ribosomal subunits of S. aureofaciens exhibits only a part of the functional properties of E. coli protein S1.
Mikulík
K
K
Smardová
J
J
Jiránová
A
A
Branny
P
P
D001426:Bacterial Proteins / Q000302:isolation & purification* / Q000378:metabolism; D004926:Escherichia coli; D006868:Hydrolysis; D007120:Immunochemistry; D007074:Immunoglobulin G / Q000032:analysis; D011072:Poly U / Q000378:metabolism; D011485:Protein Binding; D014176:Protein Biosynthesis; D012269:Ribosomal Proteins / Q000302:isolation & purification* / Q000378:metabolism; D013304:Streptomyces aureofaciens / Q000378:metabolism*; D014357:Trypsin
D001426:Bacterial Proteins; D007074:Immunoglobulin G; D012269:Ribosomal Proteins; C015494:ribosomal protein S1; C048502:ribosomal protein SS1, Streptomyces aureofaciens; D011072:Poly U; D014357:Trypsin