Developmental regulation of the chicken delta 1-crystallin gene: analysis by transgenesis and gene dissection.
We previously reviewed what we had learned about the regulation of the delta 1-crystallin gene through experiments using gene transfer techniques [Kondoh et al. (1986) Cell Differ. 19, 151-160]. It was concluded then that regulatory genetic elements for the lens-specific expression are associated with the delta 1-crystallin gene, and that these chicken elements properly function in mammalian cells. In the last couple of years, we have made significant progress in the understanding of lens-specific delta-crystallin expression. This is owing to success in transgenesis of mouse with the delta 1-crystallin gene and in functional dissection of the gene which led us to the discovery of an intragenic enhancer as the major determinant for lens-specific expression. In this article, we summarize these recent advances.
Kondoh
H
H
Department of Biophysics, Faculty of Science, University of Kyoto, Japan.
Katoh
K
K
Takahashi
Y
Y
Goto
K
K
Hayashi
S
S
Okada
T S
TS
D000818:Animals; D030801:Animals, Genetically Modified; D002642:Chick Embryo; D002645:Chickens; D003459:Crystallins / Q000235:genetics*; D005786:Gene Expression Regulation; D007908:Lens, Crystalline / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*
D003459:Crystallins