title: Room temperature, microtray chromogenic assay of factor VIII:C.
abstract: Commercial reagents of non-human origin were used to provide a room temperature, microtray chromogenic assay for factor VIII. The assay allows determination of factor VIII in the range 15-150% of normal plasma levels with a coefficient of variation of 3-4%. Haemophilic plasmas and factor VIII concentrates may also be assayed by varying the pre-dilution step. A good correlation was demonstrated with results obtained using an established one-stage clotting assay (r = 0.9985).
authors.forename: C
authors.initials: C
authors.lastname: Prowse
authors.forename: V
authors.initials: V
authors.lastname: Hornsey
authors.forename: G
authors.initials: G
authors.lastname: McKay
authors.forename: Y
authors.initials: Y
authors.lastname: Waterston
mesh_terms: D005169:Factor VIII / Q000032:analysis*; D005612:Freeze Drying; D006467:Hemophilia A / Q000097:blood; D006801:Humans; D007202:Indicators and Reagents; D008832:Microchemistry; D012016:Reference Values; D013053:Spectrophotometry / Q000379:methods; D013696:Temperature
chemical_list: D007202:Indicators and Reagents; D005169:Factor VIII