title: Prostaglandin E counteracts the gamma interferon induction of major histocompatibility complex class-II antigens on U937 cells and induction of responsiveness of U937 colony-forming cells to suppression by lactoferrin, transferrin, acidic isoferritins, and prostaglandin E.
abstract: The established human monoblast or early monocyte cell line, U937, was evaluated for modulating influences of prostaglandin E2 (PGE2) on human gamma interferon (HuIFN gamma) induction of MHC class-II (Ia) antigens on U937 cells and the HuIFN gamma induction of responsiveness of U937 colony-forming cells (CFC) to inhibition by lactoferrin (LF), transferrin (TF), acidic isoferritins (AIF), and prostaglandin E (PGE). U937 CFC were induced to a state of responsiveness to the suppressive influences of PGE by HuIFN gamma. When MHC class-II antigens were induced on U937 cells and the cells sorted on the fluorescence activated cell sorter (FACS) IV into positive and negative cells, colony formation by the MHC class-II antigen+ population of cells was suppressed by LF, TF, AIF, and PGE2. Colony formation by the sorted population of MHC class-II antigen- cells was not influenced significantly by LF, TF, AIF, or PGE2. When PGE was present in the suspension culture for 72 h with U937 cells exposed to HuIFN gamma plus indomethacin, it blocked the induction of MHC class-II antigens as well as the associated inhibition of U937 CFC by LF, TF, AIF, and PGE2.
authors.forename: W
authors.initials: W
authors.lastname: Piacibello
authors.forename: B Y
authors.initials: BY
authors.lastname: Rubin
authors.forename: H E
authors.initials: HE
authors.lastname: Broxmeyer
mesh_terms: D002460:Cell Line; D002469:Cell Separation; D015232:Dinoprostone; D005293:Ferritins / Q000494:pharmacology*; D005434:Flow Cytometry; D006410:Hematopoiesis / Q000187:drug effects*; D006412:Hematopoietic Stem Cells / Q000187:drug effects; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D006801:Humans; D007371:Interferon-gamma / Q000037:antagonists & inhibitors*; D007781:Lactoferrin / Q000494:pharmacology*; D007782:Lactoglobulins / Q000494:pharmacology*; D009000:Monocytes / Q000166:cytology*; D011458:Prostaglandins E / Q000494:pharmacology*; D014168:Transferrin / Q000494:pharmacology*
chemical_list: D000949:Histocompatibility Antigens Class II; D007782:Lactoglobulins; D011458:Prostaglandins E; D014168:Transferrin; D007371:Interferon-gamma; D005293:Ferritins; D007781:Lactoferrin; D015232:Dinoprostone