Detection of leprosy antigenuria through DOT-ELISA.
Fifty-five samples of urine from different grades of leprosy patients and normal persons were processed for detection of PGL-1 antigen through DOT-ELISA on nitrocellulose paper strips using anti-human IgG horse raddish peroxidaseconjugate. About 66.6% of the paucibacillary and 100% of the multibacillary leprosy cases were detectable through this technique on the basis of differential colour development on the strips. Possibility of its use in the field conditions has been discussed.
Singh
N B
NB
Division of Microbiology, Central Drug Research Institute, Lucknow.
Choudhary
A
A
D000942:Antigens, Bacterial / Q000652:urine*; D004797:Enzyme-Linked Immunosorbent Assay; D006017:Glycolipids / Q000652:urine*; D006801:Humans; D015440:Leprosy, Lepromatous / Q000276:immunology* / Q000652:urine; D009166:Mycobacterium leprae / Q000276:immunology*
D000942:Antigens, Bacterial; D006017:Glycolipids; C045169:phenolic glycolipid I, Mycobacterium leprae