Frequent lambda light chain gene rearrangement and expression in a Ly-1 B lymphoma with a productive kappa chain allele.
We describe here a murine Ly-1-bearing pre-B-cell tumor that, when induced for kappa light chain expression with bacterial lipopolysaccharide, also gives rise spontaneously to a few percent of cells expressing surface lambda light chains. These lambda-positive cells have undergone DNA rearrangements involving either V lambda 1 or V lambda 2 genes. Nearly all clones of lambda-bearing cells express mu and lambda on their surface (but not kappa). However, all these lambda-positive clones continue to transcribe kappa mRNA and synthesize internal kappa chains. Further, surface lambda-positive clones show JH rearrangements on one or both heavy chain chromosomes.
Hardy
R R
RR
Dangl
J L
JL
Hayakawa
K
K
Jager
G
G
Herzenberg
L A
LA
Herzenberg
L A
LA
D000818:Animals; D000950:Antigens, Ly / Q000032:analysis*; D001402:B-Lymphocytes / Q000502:physiology*; D002460:Cell Line; D005786:Gene Expression Regulation; D007147:Immunoglobulin Light Chains / Q000235:genetics*; D007145:Immunoglobulin kappa-Chains / Q000235:genetics*; D007146:Immunoglobulin lambda-Chains / Q000235:genetics*; D007148:Immunoglobulin mu-Chains / Q000235:genetics; D008223:Lymphoma / Q000235:genetics / Q000276:immunology*; D051379:Mice; D012333:RNA, Messenger / Q000235:genetics; D011995:Recombination, Genetic
D000950:Antigens, Ly; D007147:Immunoglobulin Light Chains; D007145:Immunoglobulin kappa-Chains; D007146:Immunoglobulin lambda-Chains; D007148:Immunoglobulin mu-Chains; D012333:RNA, Messenger