Yeast nuclear gene CBS2, required for translational activation of cytochrome b, encodes a basic protein of 45 kDa.
In yeast, synthesis of apocytochrome b from mitochondrial COB mRNA depends on at least three nuclear gene products. The translation stimulatory effect by two of these nuclear genes, CBS1 and CBS2, is mediated by the 5'-untranslated leader of COB mRNA. In this report, we show that CBS2 is located on chromosome IV and provide genetic evidence that the CBS2 gene encodes a polypeptide. Determination of the DNA sequence reveals a contiguous open reading frame of 1167 bp. The deduced polypeptide has a calculated molecular weight of 44.5 kDa and is characterized by a high content of positively charged amino acids. It has no significant homology to any known protein. The CBS2 gene is transcribed into low abundance mRNA species with a major transcription initiation site located 97 bp upstream from the ATG start codon next to a poly(dA-dT) stretch.
Michaelis
U
U
Institut für Genetik und Mikrobiologie der Universität München, Federal Republic of Germany.
Schlapp
T
T
Rödel
G
G
D000595:Amino Acid Sequence; D001483:Base Sequence; D002467:Cell Nucleus / Q000502:physiology; D003062:Codon; D003573:Cytochrome b Group / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics* / Q000502:physiology; D005786:Gene Expression Regulation; D005796:Genes; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010957:Plasmids; D014176:Protein Biosynthesis; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D003062:Codon; D003573:Cytochrome b Group; D004272:DNA, Mitochondrial; D005656:Fungal Proteins