Anti-hapten IgG antibodies bound to cell surface hapten: anti-IgG antibody prevents dissociation as measured with fluid phase hapten.
We investigated the dissociation by fluid phase hapten of IgG antibodies bound to cell surface hapten in the presence and absence of anti-IgG antibodies. Dissociation was quantitated with fluid phase hapten, preventing reassociation of the anti-hapten antibodies. More than 90% of the anti-hapten IgG alone was prevented from reassociation by low concentrations of fluid phase hapten (nanogram to microgram range). In contrast, no dissociation of some IgG-anti-IgG complexes could be measured even at 24 hr incubation in the presence of very large excess of fluid phase hapten (100 mg/ml). We excluded aggregate formation between anti-hapten antibodies due to cross-linking by anti-antibodies as a cause for decreased dissociability by 1) performing the experiments in large excess of anti-antibody, 2) showing that the phenomenon was independent of anti-hapten antibody density, 3) showing that decreased dissociation also occurred at 4 degrees C, and 4) showing that aggregation by protein A did induce decreased dissociability, albeit three orders of magnitude lower than the anti-antibody. It was concluded that anti-antibody directly affected the "avidity" of cell hapten bound anti-hapten IgG in an unknown manner.
Borsos
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Circolo
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Young-Cooper
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Mage
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D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000502:physiology*; D000954:Antigens, Surface / Q000276:immunology; D001666:Binding Sites, Antibody; D001789:Blood Group Antigens / Q000276:immunology; D006041:Goats; D006241:Haptens / Q000276:immunology*; D007126:Immunoglobulin Allotypes / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology / Q000378:metabolism*; D007145:Immunoglobulin kappa-Chains / Q000276:immunology; D007700:Kinetics; D046911:Macromolecular Substances; D008727:Methotrexate / Q000276:immunology; D011817:Rabbits; D012756:Sheep
D000888:Antibodies, Anti-Idiotypic; D000954:Antigens, Surface; D001789:Blood Group Antigens; D006241:Haptens; D007126:Immunoglobulin Allotypes; D007074:Immunoglobulin G; D007145:Immunoglobulin kappa-Chains; D046911:Macromolecular Substances; D008727:Methotrexate