Failure of PAF-acether to induce in vivo neutrophil migration.
The ability of PAF-acether (platelet activating factor, 1-alkyl-2-acetyl-sn-glycero-3-phosphocholine) to induce neutrophil migration was tested in the peritoneal cavities of rats and guinea-pigs and in rat skin air pouches. PAF-acether, at doses of 2 and 5 micrograms per animal, did not promote significant neutrophil migration. The ability of PAF-acether antagonists to inhibit carrageenin induced neutrophil migration in rat abdominal cavities was also investigated. Carrageenin is a substance described to cause the release of endogenous PAF-acether. Neutrophil migration was not affected by PAF-antagonists. These observations challenge the possible role of PAF-acether as a neutrophil chemotactic factor in vivo.
Cunha
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FQ
Department of Pharmacology, Faculty of Medicine, University of São Paulo, Ribeirão Preto, Brazil.
Heluy-Neto
N E
NE
Klein
A
A
Souza
G E
GE
Ferreira
S H
SH
D000818:Animals; D002351:Carrageenan; D002630:Chemotactic Factors / Q000502:physiology; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D006168:Guinea Pigs; D016209:Interleukin-8; D008297:Male; D009504:Neutrophils / Q000187:drug effects* / Q000276:immunology / Q000502:physiology; D010972:Platelet Activating Factor / Q000494:pharmacology* / Q000502:physiology; D051381:Rats; D011919:Rats, Inbred Strains
D002630:Chemotactic Factors; D016209:Interleukin-8; D010972:Platelet Activating Factor; D002351:Carrageenan