Assessing the specificity of anti-canine-factor VIII-related antigen.
Antibodies to canine factor VIII-related antigen should be monospecific to accurately study this protein's location in tissue and its structure. Because no uniformly accepted standard for monospecificity exists for anti-factor VIII-related antigen, we have compared the sensitivities of three common evaluation techniques--immunodiffusion, immunoelectrophoresis, and crossed immunoelectrophoresis. Unabsorbed rabbit anti-canine factor VIII-related antigen appeared monospecific against whole plasma by all three methods; however, multiple specificities were recognized against a plasma cryoconcentrate. These results demonstrate the insensitivity of the techniques under certain conditions, which can lead to erroneous interpretation of data.
Benson
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Dodds
W J
WJ
D000818:Animals; D000918:Antibody Specificity; D000941:Antigens / Q000276:immunology*; D004285:Dogs; D005069:Evaluation Studies as Topic; D005169:Factor VIII / Q000276:immunology*; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D007123:Immunoelectrophoresis, Two-Dimensional; D007163:Immunosorbent Techniques; D011817:Rabbits; D014841:von Willebrand Factor
D000941:Antigens; D014841:von Willebrand Factor; D005169:Factor VIII