title: Sterol composition of a delta 5,7-sterol-rich strain of Saccharomyces cerevisiae during batch growth.
abstract: Sterol composition was examined during batch growth on complex media containing ethanol, molasses or glucose as the carbon source. The molasses-grown cells exhibited a balanced sterol composition throughout growth, maintaining the proportion of ergosterol to 24:28-dehydroergosterol equal to 1.4. The negative effect of glucose on sterol synthesis manifested itself by decreasing the accumulation of 24:28-dehydroergosterol and total sterols but not of ergosterol. Using ethanol as the sole carbon source, a large amount of 24:28-dehydroergosterol accumulated, partly at the expense of other sterols. The gradual addition of nitrogen source during growth significantly decreased the accumulation of ergosterol, 24:28-dehydroergosterol and of total sterols. A general scheme of regulation of sterol synthesis in baker's yeast is presented.
authors.affiliation: Institute of Microbiology, Czechoslovak Academy of Sciences, Prague.
authors.forename: C
authors.initials: C
authors.lastname: Novotný
authors.forename: B
authors.initials: B
authors.lastname: Bĕhalová
authors.forename: R
authors.initials: R
authors.lastname: Struzinský
authors.forename: M
authors.initials: M
authors.lastname: Novák
authors.forename: J
authors.initials: J
authors.lastname: Zajícek
mesh_terms: D000641:Ammonia; D000693:Anaerobiosis; D002244:Carbon / Q000378:metabolism; D003470:Culture Media; D004875:Ergosterol / Q000031:analogs & derivatives* / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000378:metabolism; D000431:Ethanol; D005947:Glucose; D006903:Hydroxymethylglutaryl CoA Reductases / Q000378:metabolism; D008965:Molasses; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000254:growth & development* / Q000378:metabolism; D013261:Sterols / Q000096:biosynthesis / Q000378:metabolism
chemical_list: D003470:Culture Media; D013261:Sterols; C010905:dehydroergosterol; D000431:Ethanol; D002244:Carbon; D000641:Ammonia; D006903:Hydroxymethylglutaryl CoA Reductases; D005947:Glucose; D004875:Ergosterol