title: DNA polymerase of bacteriophage T4 is an autogenous translational repressor.
abstract: In bacteriophage T4 the protein product of gene 43 (gp43) is a multifunctional DNA polymerase that is essential for replication of the phage genome. The protein harbors DNA-binding, deoxyribonucleotide-binding, DNA-synthesizing (polymerase) and 3'-exonucleolytic (editing) activities as well as a capacity to interact with several other T4-induced replication enzymes. In addition, the T4 gp43 is a repressor of its own synthesis in vivo. We show here that this protein is an autogenous repressor of translation, and we have localized its RNA-binding sequence (translational operator) to the translation initiation domain of gene 43 mRNA. This mechanism for regulation of T4 DNA polymerase expression underscores the ubiquity of translational repression in the control of T4 DNA replication. Many T4 DNA polymerase accessory proteins and nucleotide biosynthesis enzymes are regulated by the phage-induced translational repressor regA, while the T4 single-stranded DNA-binding protein (T4 gp32) is, like gp43, autogenously regulated at the translational level.
authors.affiliation: Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston 29425.
authors.forename: M
authors.initials: M
authors.lastname: Andrake
authors.forename: N
authors.initials: N
authors.lastname: Guild
authors.forename: T
authors.initials: T
authors.lastname: Hsu
authors.forename: L
authors.initials: L
authors.lastname: Gold
authors.forename: C
authors.initials: C
authors.lastname: Tuerk
authors.forename: J
authors.initials: J
authors.lastname: Karam
mesh_terms: D001483:Base Sequence; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D014176:Protein Biosynthesis; D011499:Protein Processing, Post-Translational; D012097:Repressor Proteins; D013604:T-Phages / Q000201:enzymology*; D014157:Transcription Factors
chemical_list: D012097:Repressor Proteins; D014157:Transcription Factors; D004259:DNA-Directed DNA Polymerase