title: Anti-hapten IgG antibodies bound to cell surface hapten: anti-IgG antibody prevents dissociation as measured with fluid phase hapten.
abstract: We investigated the dissociation by fluid phase hapten of IgG antibodies bound to cell surface hapten in the presence and absence of anti-IgG antibodies. Dissociation was quantitated with fluid phase hapten, preventing reassociation of the anti-hapten antibodies. More than 90% of the anti-hapten IgG alone was prevented from reassociation by low concentrations of fluid phase hapten (nanogram to microgram range). In contrast, no dissociation of some IgG-anti-IgG complexes could be measured even at 24 hr incubation in the presence of very large excess of fluid phase hapten (100 mg/ml). We excluded aggregate formation between anti-hapten antibodies due to cross-linking by anti-antibodies as a cause for decreased dissociability by 1) performing the experiments in large excess of anti-antibody, 2) showing that the phenomenon was independent of anti-hapten antibody density, 3) showing that decreased dissociation also occurred at 4 degrees C, and 4) showing that aggregation by protein A did induce decreased dissociability, albeit three orders of magnitude lower than the anti-antibody. It was concluded that anti-antibody directly affected the "avidity" of cell hapten bound anti-hapten IgG in an unknown manner.
authors.forename: T
authors.initials: T
authors.lastname: Borsos
authors.forename: A
authors.initials: A
authors.lastname: Circolo
authors.forename: G O
authors.initials: GO
authors.lastname: Young-Cooper
authors.forename: R
authors.initials: R
authors.lastname: Mage
mesh_terms: D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000502:physiology*; D000954:Antigens, Surface / Q000276:immunology; D001666:Binding Sites, Antibody; D001789:Blood Group Antigens / Q000276:immunology; D006041:Goats; D006241:Haptens / Q000276:immunology*; D007126:Immunoglobulin Allotypes / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology / Q000378:metabolism*; D007145:Immunoglobulin kappa-Chains / Q000276:immunology; D007700:Kinetics; D046911:Macromolecular Substances; D008727:Methotrexate / Q000276:immunology; D011817:Rabbits; D012756:Sheep
chemical_list: D000888:Antibodies, Anti-Idiotypic; D000954:Antigens, Surface; D001789:Blood Group Antigens; D006241:Haptens; D007126:Immunoglobulin Allotypes; D007074:Immunoglobulin G; D007145:Immunoglobulin kappa-Chains; D046911:Macromolecular Substances; D008727:Methotrexate