Elimination kinetics of circulating antigens and immune complexes. II. Hepatic and extrahepatic elimination of chemically modified albumin preparations in relation to their non-specific physicochemical properties.
Formaldehyde-treated human serum albumin (fHSA) was analysed and compared with dinitrophenylated human serum albumin (DNP35HSA) with respect to net charge, hydrophobicity, and state of aggregation. The blood clearance and tissue distribution of intravenously injected fHSA was studied in mice. Similarly to DNP-HSA, fHSA was rapidly cleared from the blood stream owing to hepatic uptake and to extrahepatic deposition. We found no evidence supporting that fHSA was eliminated from the blood by hydrophobic interaction with cell or tissue surfaces. The blood clearance rate and the hepatic uptake of fHSA was not influenced by the presence of formylated peptides, indicating that receptors for formylated peptides were not involved in the handling of circulating fHSA.
Skogh
T
T
Department of Rheumatology, University of Linköping, Sweden.
Eskild
W
W
Sundqvist
T
T
Berg
T
T
D000818:Animals; D004140:Dinitrophenols / Q000493:pharmacokinetics*; D005455:Fluorescent Antibody Technique; D005557:Formaldehyde / Q000493:pharmacokinetics*; D008099:Liver / Q000378:metabolism*; D008657:Metabolic Clearance Rate; D051379:Mice; D008807:Mice, Inbred BALB C; D012709:Serum Albumin / Q000493:pharmacokinetics*; D014018:Tissue Distribution
D004140:Dinitrophenols; D012709:Serum Albumin; C058064:dinitrophenyl-human serum albumin conjugate; D005557:Formaldehyde