[Optimization of methods for immobilizing antibodies against the carcino-embryonic antigen on insoluble matrices. Equilibrium parameters of the interaction of the immobilized antibodies with the antigen].
To obtain highly efficient immunosorbents for solid-phase immunoassay and affinity chromatography, methods for immobilization of antibodies against the carcino-embryonic antigen (CEA) on insoluble matrices were optimized. The immunosorbents obtained were characterized by equilibrium parameters of the reaction between immobilized anti-CEA and CEA calculated from rather a simple kinetic model. This model describes the interaction of the monovalent antigen with two independent types of binding sites. The role of some amino acid residues of anti-CEA in the interaction with CEA was investigated. The effects of immobilization density and the spacer arm length on the functional properties of the immobilized antibodies were studied. The optimal immunosorbent was used to purify 125I-CEA by immunoaffinity chromatography.
Guzov
V M
VM
Usanov
S A
SA
D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D000937:Antigen-Antibody Reactions; D002272:Carcinoembryonic Antigen / Q000276:immunology*; D002846:Chromatography, Affinity; D006639:Histidine / Q000032:analysis; D006801:Humans; D007074:Immunoglobulin G / Q000302:isolation & purification; D007163:Immunosorbent Techniques; D013438:Sulfhydryl Compounds / Q000032:analysis; D014443:Tyrosine / Q000032:analysis
D000911:Antibodies, Monoclonal; D002272:Carcinoembryonic Antigen; D007074:Immunoglobulin G; D013438:Sulfhydryl Compounds; D014443:Tyrosine; D006639:Histidine
Optimizatsiia metodov immobilizatsii antitel protiv rakovo- émbrional'nogo antigena na nerastvorimykh matritsakh. Ravnovesnye parametry vzaimodeĭstviia immobilizovannykh antitel s antigenom.