Characterization of an antibody directed against a 128 kDa glycoprotein involved in the thrombogenicity of the elastin-associated microfibrils.
We have developed a monospecific antiserum directed against a major glycoprotein in the elastin-associated microfibrils with an apparent molecular mass of 128 kDa (GP 128). When immunoblotting or enzyme-linked immunosorbent microassay was used, its IgGs recognized thrombospondin in a platelet lysate, but did not react with several basement-membrane-derived macromolecules, nor with plasma fibronectin. Similar patterns of immunofluorescence and immunoperoxidase were found after incubation of endothelial cells with either anti-GP 128 or anti-(platelet thrombospondin) IgGs. Both antibodies inhibited the microfibrils- and thrombin-induced platelet aggregation, and were without effect on the aggregation by other inducers. These results confirm that there is an antigenic homology between GP 128 and thrombospondin.
Fauvel-Lafeve
F
F
INSERM Unit 150, CNRS UA 334, Hôpital St. Louis, Paris, France.
Picard
P
P
Godeau
G
G
Legrand
Y J
YJ
D001777:Blood Coagulation; D002478:Cells, Cultured; D003285:Contractile Proteins / Q000276:immunology* / Q000378:metabolism; D004549:Elastin; D004730:Endothelium, Vascular / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D016326:Extracellular Matrix Proteins; D005455:Fluorescent Antibody Technique; D006023:Glycoproteins / Q000276:immunology; D007106:Immune Sera / Q000032:analysis*; D015151:Immunoblotting; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000276:immunology; D046911:Macromolecular Substances; D009131:Muscle, Smooth, Vascular / Q000378:metabolism*; D010974:Platelet Aggregation; D000072260:RNA Splicing Factors; D019699:Thrombospondins
D003285:Contractile Proteins; D016326:Extracellular Matrix Proteins; D006023:Glycoproteins; D007106:Immune Sera; D007074:Immunoglobulin G; D046911:Macromolecular Substances; D000072260:RNA Splicing Factors; D019699:Thrombospondins; C016598:microfibrillar protein; D004549:Elastin