Separation of isoenzymes of citrate synthase and isocitrate dehydrogenase by fast protein liquid chromatography.
Fast protein liquid chromatography (FPLC) has been shown to be a rapid and effective method of separating isoenzymes of citrate synthase and isocitrate dehydrogenase in extracts of Pseudomonas aeruginosa and Acinetobacter calcoaceticus. The advantages of FPLC over conventional methods of fractionation are discussed and it is suggested that this may be a valuable and more general technique for isoenzyme resolution.
Mitchell
C G
CG
O'Neil
S
S
Reeves
H C
HC
Weitzman
P D
PD
D000150:Acinetobacter / Q000201:enzymology; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002950:Citrate (si)-Synthase / Q000302:isolation & purification*; D007521:Isocitrate Dehydrogenase / Q000302:isolation & purification*; D007527:Isoenzymes / Q000302:isolation & purification*; D007652:Oxo-Acid-Lyases / Q000302:isolation & purification*; D011550:Pseudomonas aeruginosa / Q000201:enzymology
D007527:Isoenzymes; D007521:Isocitrate Dehydrogenase; D002950:Citrate (si)-Synthase; D007652:Oxo-Acid-Lyases