title: Cloning the FnuDI, NaeI, NcoI and XbaI restriction-modification systems.
abstract: Methyltransferase genes from the FnuDI, NaeI, NcoI, and XbaI restriction-modification systems have been isolated in Escherichia coli by 'shot-gun' cloning bacterial DNA fragments into plasmid vectors and selecting for protectively modified molecules that resist digestion by the corresponding restriction endonuclease.
authors.affiliation: New England Biolabs, Inc., Beverly, MA 01915.
authors.forename: E M
authors.initials: EM
authors.lastname: Van Cott
authors.forename: G G
authors.initials: GG
authors.lastname: Wilson
mesh_terms: D003001:Cloning, Molecular; D015257:DNA-Cytosine Methylases / Q000235:genetics*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D005673:Fusobacterium / Q000201:enzymology / Q000235:genetics*; D005796:Genes; D005798:Genes, Bacterial; D009615:Nocardia / Q000201:enzymology / Q000235:genetics*; D011994:Recombinant Proteins / Q000235:genetics; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific) / Q000235:genetics*; D014974:Xanthomonas / Q000201:enzymology / Q000235:genetics*
chemical_list: D011994:Recombinant Proteins; C059641:DNA modification methylase NaeI; C059640:DNA modification methylase NcoI; C059639:DNA modification methylase XbaI; D015257:DNA-Cytosine Methylases; C027823:Cytosine 5-methyltransferase; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific); C025580:endodeoxyribonuclease XBAI; D015252:Deoxyribonucleases, Type II Site-Specific; C024403:GGCC-specific type II deoxyribonucleases