Morphological changes in pancreatic B-cells after intravenous administration of forskolin.
The morphological changes in rat pancreatic islets were studied after intravenous injection of forskolin (1.5 mg/kg), an activator of adenylate cyclase, to male Wistar rats. A high amount of insulin containing granules was observed on PAP immunostained paraffin sections. Ultrastructural study of islet B-cells demonstrated enlarged perinuclear space, dilated cisternae of rough endoplasmic reticulum and activated Golgi complex with numerous vesicles. The results suggest that intravenous administration of forskolin produces a modest increase of B-cell synthetic activity in the pancreatic islets of rats. It is assumed that forskolin affects pancreatic B-cells via increased cAMP.
Zafirova
M
M
Katedra po Anatomia, Histologia i Embryologia, Medicinska Akademia, Sofia.
Ogneva
V
V
Petkov
P
P
Koparanova
O
O
D000818:Animals; D005576:Colforsin / Q000008:administration & dosage / Q000494:pharmacology*; D003594:Cytoplasmic Granules / Q000187:drug effects / Q000648:ultrastructure; D007275:Injections, Intravenous; D007515:Islets of Langerhans / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure*; D008297:Male; D008854:Microscopy, Electron; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values
D005576:Colforsin