[Comparison of methods of detection of bovine adenovirus serotype 3 in infected culture of calf kidney cells (MDBK)].
The comparative study of the dynamics of the main antigen (hexon) and viral DNA of the bovine adenovirus type 3 accumulation in the established cell line MDBK under the conditions of single- or multistep cycle of infection has been undertaken. The quantitative immunoelectrophoresis and immunoenzyme assay detected the viral antigens on the late stages of infection in the period of cellular monolayer degradation. The immunofluorescence reaction and histochemical immunoenzyme method detected the antigen in the infected cells concurrently with the primary expression of the viral cytopathic effect. The reaction of the spot molecular hybridization with the [32P]-DNA probes detected the viral DNA considerably earlier than the antigen was detected by the immunological methods, before the appearance of degenerative changes in the infected cells. Preference of the immunoenzyme assay and DNA-probes in diagnosis of the virus are discussed.
Kaliuzhnaia
A N
AN
Trifonov
V D
VD
Zil'berman
M I
MI
Zalmanzon
E S
ES
Kaledin
A S
AS
D000256:Adenoviridae / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002417:Cattle; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D004279:DNA, Viral / Q000032:analysis; D007124:Immunoenzyme Techniques; D007668:Kidney
D000956:Antigens, Viral; D004279:DNA, Viral
Sopostavlenie metodov obnaruzheniia adenovirusa krupnogo rogatogo skota 3-go serotipa v zarazhennoĭ kul'ture perevivaemykh kletok pochki telenka (MDBK).