[Enucleation and reconstruction of murine L-cell fibroblasts].
Two types of teflon chambers are suggested for the procedures of enucleation and reconstruction of cells cultivated in the monolayer. The first chamber simplifies the enucleation and permits performing it on the standard cover glasses with a small volume of medium with cytochalasin B. The second chamber permits purifying the karyoplast fraction from intact cells by their adhesion on the cover glasses. Then the karyoplasts are sedimented by centrifugation on cytoplasts located on the cover glasses. This procedure promotes sticking of fragments and their subsequent fusion in the presence of polyethylene glycol. In total the number of reconstructed cells increases many times.
Neverova
M E
ME
Kazakova
O M
OM
D000818:Animals; D002458:Cell Fractionation / Q000379:methods*; D002467:Cell Nucleus / Q000648:ultrastructure*; D003584:Cytological Techniques / Q000295:instrumentation; D015194:Diffusion Chambers, Culture; D007739:L Cells / Q000648:ultrastructure*; D051379:Mice; D011138:Polytetrafluoroethylene
D011138:Polytetrafluoroethylene
Enukleatsiia i rekonstruktsiia myshinykh fibroblastov linii L.