title: Pancreatic proglucagon processing: isolation and structures of glucagon and glucagon-like peptide from gene I.
abstract: The anglerfish endocrine pancreas expresses two different genes for preproglucagon. The regions of the two proglucagons that correspond to glucagon have different sequences, as do the two glucagon-like peptides (GLPs). The products derived from processing the more abundant proglucagon-II have recently been determined. However, it was not known whether proglucagon-I was processed to similar products. The two major biologically active products of preproglucagon-I processing (glucagon-I and GLP-I) have now been purified to homogeneity. Their structures were determined using automated gas phase Edman degradation, tryptic mapping, and fast atom bombardment mass spectrometry. The preproglucagon-I-processing sites were identified. Glucagon-I represents residues 53-81, and GLP-I corresponds to preproglucagon-I-(91-124) (numbering from the initiator Met).
authors.affiliation: Biochemistry Department, Purdue University, West Lafayette, Indiana 47907.
authors.forename: R
authors.initials: R
authors.lastname: Nichols
authors.forename: T D
authors.initials: TD
authors.lastname: Lee
authors.forename: P C
authors.initials: PC
authors.lastname: Andrews
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000235:genetics; D005399:Fishes / Q000378:metabolism*; D005934:Glucagon / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D004763:Glucagon-Like Peptides; D007515:Islets of Langerhans / Q000378:metabolism*; D008969:Molecular Sequence Data; D010446:Peptide Fragments; D010455:Peptides / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D052336:Proglucagon; D011498:Protein Precursors / Q000235:genetics* / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D014357:Trypsin
chemical_list: D010446:Peptide Fragments; D010455:Peptides; D011498:Protein Precursors; D052336:Proglucagon; D004763:Glucagon-Like Peptides; C015236:glucagon-like-immunoreactivity; D004247:DNA; D005934:Glucagon; D014357:Trypsin