title: [Purification of restriction endonuclease EcoRII using monoclonal antibodies].
abstract: Monoclonal antibodies against EcoRII endonuclease were obtained after immunization of two BALB/c mice with a homogeneous enzyme prepared by conventional methods. IgG from ascitic fluid was purified and coupled to CNBr-activated Sepharose 4B to give a specific column used to isolate EcoRII endonuclease. The isolated EcoRII endonuclease produced a single band during SDS gel electrophoresis.
authors.forename: V G
authors.initials: VG
authors.lastname: Kosykh
authors.forename: Iu A
authors.initials: IuA
authors.lastname: Mantsygin
authors.forename: N V
authors.initials: NV
authors.lastname: Sviatukhina
authors.forename: I V
authors.initials: IV
authors.lastname: Vitenene
authors.forename: Ia I
authors.initials: IaI
authors.lastname: Bur'ianov
mesh_terms: D000911:Antibodies, Monoclonal; D015252:Deoxyribonucleases, Type II Site-Specific / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology*; D007163:Immunosorbent Techniques
chemical_list: D000911:Antibodies, Monoclonal; C046545:CCWGG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific
vernacular_title: Ochistka restriltsionnoĭ éndonukleazy EcoRII s ispol'zovaniem monoklonal'nykh antitel.