Role of hepatocytes in the uptake of IgA and IgA-containing immune complexes in mice.
The role of parenchymal and nonparenchymal mouse liver cells in the uptake of polymeric IgA (pIgA) and pIgA-containing immune complexes (IC) of low mol. wt (less than 1 x 10(6)) was studied. As detected by immunofluorescence and immunoelectron microscopy, pIgA were bound on the surface of isolated hepatocytes. Following the injection of radiolabeled pIgA or pIgA-IC into mice, the total radioactivity recovered from isolated liver cells was preferentially associated with parenchymal cells. The ability to inhibit the transport of pIgA and pIgA-IC by pIgA of an irrelevant specificity suggests that pIgA-IC and pIgA are bound and transported by the same mechanism. These results indicate that mouse hepatocytes are involved in the uptake and hepatobiliary transport of pIgA and pIgA-IC of low mol. wt.
Phillips
J O
JO
Department of Microbiology, University of Alabama, Birmingham 35294.
Komiyama
K
K
Epps
J M
JM
Russell
M W
MW
Mestecky
J
J
D000818:Animals; D000936:Antigen-Antibody Complex / Q000378:metabolism*; D001646:Bile / Q000276:immunology; D001692:Biological Transport / Q000187:drug effects; D002499:Centrifugation, Density Gradient; D003513:Cycloheximide / Q000494:pharmacology; D005455:Fluorescent Antibody Technique; D007070:Immunoglobulin A / Q000378:metabolism*; D008099:Liver / Q000276:immunology*; D008854:Microscopy, Electron; D009115:Muridae / Q000276:immunology*
D000936:Antigen-Antibody Complex; D007070:Immunoglobulin A; D003513:Cycloheximide