[The use of monoclonal antibodies for the quantitative determination of myoglobin by the solid phase immunoenzyme "sandwich" assay].
A procedure of solid phase single step immunoenzyme assay of the "sandwich" type involving monoclonal antibodies was developed to estimate myoglobin in blood serum of patients, 30 stable hybridomic clones. producing antibodies to not less than six various epitopes of myoglobin, were obtained. Content of myoglobin in blood serum was studied using antibodies of the IgG type to two various epitopes of myoglobin as well as of their conjugates with horseradish peroxidase. The procedure developed enabled to estimate myoglobin in blood serum at concentrations 2-1,600 ng/ml and to analyze 120 preparations within 3 hrs.
Iuronen
E I
EI
Viĭksmaa
M G
MG
Mikel'saar
A V
AV
D000818:Animals; D000911:Antibodies, Monoclonal; D006801:Humans; D006825:Hybridomas; D007124:Immunoenzyme Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D009211:Myoglobin / Q000032:analysis*
D000911:Antibodies, Monoclonal; D009211:Myoglobin
Ispol'zovanie monoklonal'nykh antitel dlia kolichestvennogo opredeleniia mioglobina s pomoshch'iu tverdofaznogo immunofermentnogo analiza tipa sandvich.