Characterization of tissue amyloid by immunofluorescence microscopy.
Immunohistochemical classification of amyloid type was possible in 44 of 50 (88%) patients as judged by the concordance of immunofluorescence, clinical, serum, and urine immunoelectrophoresis, and bone marrow data. In frozen tissue sections incubated with a panel of antisera monospecific for immunoglobulin heavy chains, kappa and lambda light chains, and amyloid-A-related protein, the amyloid was classified as AL in 20 and AA in 24. In 6 patients the amyloid could not be classified because of the absence of reactivity in 2 and overlap staining in 4. The findings indicate that routine immunofluorescence examination of diagnostic biopsies is an important adjunct in the classification of amyloid.
Gallo
G R
GR
Feiner
H D
HD
Chuba
J V
JV
Beneck
D
D
Marion
P
P
Cohen
D H
DH
D000328:Adult; D000368:Aged; D000686:Amyloidosis / Q000145:classification* / Q000276:immunology; D001853:Bone Marrow / Q000276:immunology; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007124:Immunoenzyme Techniques; D007147:Immunoglobulin Light Chains / Q000378:metabolism; D007668:Kidney / Q000276:immunology; D007678:Kidney Glomerulus / Q000276:immunology; D008297:Male; D008875:Middle Aged; D000685:Serum Amyloid A Protein / Q000378:metabolism
D007147:Immunoglobulin Light Chains; D000685:Serum Amyloid A Protein