title: The irreversibility of thiamin transport in Saccharomyces cerevisiae.
abstract: Neither exit nor counterflow efflux of thiamin, taken up previously by an active transport, were found in Saccharomyces cerevisiae, in either the wild type or a mutant with a lower rate of thiamin phosphorylation. Complete inhibition of thiamin phosphorylation by oxythiamin did not lead to any release of thiamin taken up by the cell.
authors.affiliation: Department of Biochemistry and Microbiology, Institute of Chemical Technology, Prague.
authors.forename: T
authors.initials: T
authors.lastname: Ruml
authors.forename: L
authors.initials: L
authors.lastname: Silhánková
authors.forename: P
authors.initials: P
authors.lastname: Rauch
mesh_terms: D001693:Biological Transport, Active / Q000187:drug effects; D007700:Kinetics; D009154:Mutation; D010119:Oxythiamine / Q000494:pharmacology; D010766:Phosphorylation; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D013831:Thiamine / Q000037:antagonists & inhibitors / Q000378:metabolism*
chemical_list: D010119:Oxythiamine; D013831:Thiamine