H2a-specific proteolysis as a unique probe in the analysis of the histone octamer.
We have utilized the H2a-specific protease as a unique probe to investigate the nature of the interactions between the protein subunits which form the core histone octamer. Upon incubation in high ionic strength media this protease, normally found tightly associated with isolated calf thymus chromatin, releases the 15 COOH-terminal amino acids of histone H2a by specifically cleaving the H2a polypeptide between Val114 and Leu115, yielding cleaved H2a (cH2a) and a free pentadecapeptide (Eickbush, T. H., Watson, D. K., and Moudrianakis, E. N. (1976) Cell 9, 785-792). We find that removal of this pentadecapeptide results in a marked dissociation of the octamer into its H2a:H2b dimer and H3:H4 tetramer subunits. Reconstitution experiments indicate that cH2a is capable of forming a dimer with H2b, but this cH2a:H2b dimer has a substantially lower affinity for the H3:H4 tetramer than native H2a:H2b dimer. Kinetic studies of H2a cleavage in high ionic strength solutions demonstrate that H2a molecules in the octamer are relatively resistant to proteolytic attack compared to H2a molecules in the dimer. The extent of this resistance, in response to various experimental parameters, is directly correlated to the strength of interaction between the H2a:H2b dimer and H3:H4 tetramer subunits. These reconstitution and kinetic experiments suggest that the histone domains proximal to the H2a cleavage site have an important function in maintaining the association of the histone octamer subunits.
Eickbush
T H
TH
Biology Department, Johns Hopkins University, Baltimore, Maryland 21218.
Godfrey
J E
JE
Elia
M C
MC
Moudrianakis
E N
EN
D000818:Animals; D002417:Cattle; D010450:Endopeptidases / Q000378:metabolism*; D006657:Histones / Q000378:metabolism*; D046911:Macromolecular Substances; D015335:Molecular Probes; D011108:Polymers; D013950:Thymus Gland / Q000032:analysis; D014508:Urea / Q000494:pharmacology
D006657:Histones; D046911:Macromolecular Substances; D015335:Molecular Probes; D011108:Polymers; D014508:Urea; D010450:Endopeptidases; C021361:histone proteases