Labelling of a T lymphocyte subpopulation with monoclonal antibody bound to colloidal gold. Correlation with the indirect immunofluorescence method.
A method of direct labelling of human lymphocytes CD8 with a monoclonal antibody bound to colloidal gold particles is described. Results of labelling of isolated lymphocytes and of cells of leucocyte concentrate from haemolysed blood correlated well with those obtained by indirect immunofluorescence. Owing to its simplicity and rapidity, the described method is particularly suitable for clinical diagnostics.
Korcáková
L
L
Institute of Clinical and Experimental Medicine, Praha.
Sálková
J
J
Lejnar
J
J
Kaslík
J
J
Raska
I
I
D000911:Antibodies, Monoclonal; D003102:Colloids; D005455:Fluorescent Antibody Technique; D006046:Gold; D006801:Humans; D013601:T-Lymphocytes / Q000145:classification* / Q000276:immunology
D000911:Antibodies, Monoclonal; D003102:Colloids; D006046:Gold