Purification and characterization of an inhibitor of plasminogen activator released by rat mammary adenocarcinoma cells.
An inhibitor of plasminogen activator (PA) secreted by a tumorigenic, but non-metastatic, rat mammary adenocarcinoma cell line has been purified to apparent homogeneity and characterized. It strongly inhibited human urokinase, but was 100 times less potent in inhibiting bovine trypsin and had no effect on plasmin or thrombin. A secreted, urokinase-type PA (Mr 48 000) and a cell-associated PA from a metastatic rat adenocarcinoma cell line were also strongly inhibited. In contrast, a tissue-type PA (Mr 66 000), secreted by human melanoma cells, was only slightly inhibited. Purified inhibitor showed a band of Mr 66 000 in sodium dodecyl sulphate/polyacrylamide gel electrophoresis and an isoelectric point of 4.5 after chromatofocusing. The inhibition of human urokinase was non-competitive.
Grant
A J
AJ
Ramshaw
I A
IA
Badenoch-Jones
P
P
Eichner
R D
RD
Hunt
N H
NH
D000230:Adenocarcinoma / Q000201:enzymology* / Q000378:metabolism; D000818:Animals; D002478:Cells, Cultured; D005260:Female; D007700:Kinetics; D008325:Mammary Neoplasms, Experimental / Q000201:enzymology* / Q000378:metabolism; D009362:Neoplasm Metastasis; D010960:Plasminogen Activators / Q000037:antagonists & inhibitors*; D015849:Plasminogen Inactivators; D011480:Protease Inhibitors / Q000302:isolation & purification* / Q000378:metabolism; D051381:Rats; D013379:Substrate Specificity; D014568:Urokinase-Type Plasminogen Activator / Q000037:antagonists & inhibitors
D015849:Plasminogen Inactivators; D011480:Protease Inhibitors; D010960:Plasminogen Activators; D014568:Urokinase-Type Plasminogen Activator