Monoamine oxidase B(MAO-B) is the major catalyst for 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) oxidation in human brain and other tissues.
A new in vitro radiometric method has been developed for the direct assay of the oxidation of 1-methyl-4-phenyl-1,2,3,6-tetrahydrophyridine (MPTP) to its main neurotoxic metabolite 1-methyl-4-phenylpyridinium. This assay has been used to show that the rate of oxidation of MPTP parallels that of phenylethylamine in a range of human and rodent tissues, providing strong evidence that this reaction is predominantly catalysed by monoamine oxidase B (MAO-B). In human brain the reaction was inhibited by selective doses of the MAO-B inhibitor (-)-deprenyl. When dopamine was added to the incubation mixture, products of MPTP oxidation appeared to form a complex with it.
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D015632:1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine; D015655:1-Methyl-4-phenylpyridinium; D000818:Animals; D001921:Brain / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D007527:Isoenzymes / Q000378:metabolism*; D051379:Mice; D008995:Monoamine Oxidase / Q000378:metabolism*; D010084:Oxidation-Reduction; D010627:Phenethylamines / Q000378:metabolism; D011725:Pyridines / Q000378:metabolism*; D011726:Pyridinium Compounds / Q000378:metabolism; D051381:Rats; D012642:Selegiline / Q000494:pharmacology; D013045:Species Specificity
D007527:Isoenzymes; D010627:Phenethylamines; D011725:Pyridines; D011726:Pyridinium Compounds; D012642:Selegiline; C029261:phenethylamine; D015632:1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine; D008995:Monoamine Oxidase; D015655:1-Methyl-4-phenylpyridinium