Influence of the cultivation, devitalization and preservation of Staphylococcus aureus ATCC 12598 on the activity of cell-bound protein A.
Different culture conditions, devitalizing treatments, and preservation procedures were tested for the production of protein A-bearing cells of Staphylococcus aureus ATCC 12598. Native cells with the highest IgG-binding activity were obtained after cultivation at 37 degrees C for 24-48 h in enriched media. Devitalization by ethanol, 1-propanol, formaldehyde, glutardialdehyde, chloramine T, and by heat, reduced the protein A activity depending on the duration of treatment. The protein A content of devitalized cells were best preserved by storage at -20 degrees C or by lyophilization. Staphylococcal preparations with a stable IgG-binding activity of 20 mg/g bacteria, which are very suitable for coagglutination tests, were produced by culture in TSB medium, followed by devitalization with 1-propanol and lyophilization.
Ansorg
R A
RA
Zarifoglu
F I
FI
D000433:1-Propanol / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002700:Chloramines / Q000494:pharmacology; D003470:Culture Media; D000431:Ethanol / Q000494:pharmacology; D005557:Formaldehyde / Q000494:pharmacology; D005976:Glutaral / Q000494:pharmacology; D006358:Hot Temperature; D007074:Immunoglobulin G; D007202:Indicators and Reagents; D007700:Kinetics; D013205:Staphylococcal Protein A / Q000378:metabolism*; D013211:Staphylococcus aureus / Q000187:drug effects / Q000254:growth & development*; D014105:Tosyl Compounds
D000900:Anti-Bacterial Agents; D002700:Chloramines; D003470:Culture Media; D007074:Immunoglobulin G; D007202:Indicators and Reagents; D013205:Staphylococcal Protein A; D014105:Tosyl Compounds; C016300:chloramine-T; D005557:Formaldehyde; D000431:Ethanol; D000433:1-Propanol; D005976:Glutaral