title: The in-vitro degradation at 37 degrees C of vancomycin in serum, CAPD fluid and phosphate-buffered saline.
abstract: Solutions of vancomycin in phosphate-buffered saline, peritoneal dialysis effluent fluid and human serum were incubated at 37 degrees C for ten days and sampled at daily intervals. The samples were assayed for vancomycin content by a microbiological assay, HPLC and polarisation fluoroimmunoassay (Abbott TDX). The results obtained by HPLC and microbiological assay agreed well and indicated approximately 50% loss over ten days in serum and buffered saline and over 70% loss in dialysate. TDX results indicated losses of only 20% and 40%, respectively. Degradation products were prepared from vancomycin by acid hydrolysis and purified by HPLC. These purified products were shown to cross-react in the TDX assay. It is suggested that the TDX assay becomes non-specific in the presence of vancomycin breakdown products and thus overestimates true vancomycin concentrations.
authors.affiliation: Department of Medical Microbiology, Southmead Hospital, Bristol, UK.
authors.forename: L O
authors.initials: LO
authors.lastname: White
authors.forename: R
authors.initials: R
authors.lastname: Edwards
authors.forename: H A
authors.initials: HA
authors.lastname: Holt
authors.forename: A M
authors.initials: AM
authors.lastname: Lovering
authors.forename: R G
authors.initials: RG
authors.lastname: Finch
authors.forename: D S
authors.initials: DS
authors.lastname: Reeves
mesh_terms: D001431:Bacteriological Techniques; D002851:Chromatography, High Pressure Liquid; D015200:Fluoroimmunoassay; D006801:Humans; D010531:Peritoneal Dialysis, Continuous Ambulatory; D012965:Sodium Chloride; D014640:Vancomycin / Q000097:blood / Q000378:metabolism*
chemical_list: D012965:Sodium Chloride; D014640:Vancomycin