Rapid hydrophobic grid membrane filter-enzyme-labeled antibody procedure for identification and enumeration of Escherichia coli O157 in foods.
An O-antigen-specific monoclonal antibody, labeled by horseradish peroxidase-protein A, was used in a hydrophobic grid membrane filter-enzyme-labeled antibody method for rapid detection of Escherichia coli O157 in foods. The method yielded presumptive identification within 24 h and recovered, on average, 95% of E. coli O157:H7 artificially inoculated into comminuted beef, veal, pork, chicken giblets, and chicken carcass washings. In food samples from two outbreaks involving E. coli O157:H7, the organism was isolated at levels of up to 10(3)/g. The lower limit of sensitivity was 10 E. coli O157 per g of meat. Specific typing for E. coli O157:H7 can be achieved through staining with labeled H7 antiserum or tube agglutination.
Todd
E C
EC
Bureau of Microbial Hazards, Health Protection Branch, Tunney's Pasture, Ottawa, Ontario, Canada.
Szabo
R A
RA
Peterkin
P
P
Sharpe
A N
AN
Parrington
L
L
Bundle
D
D
Gidney
M A
MA
Perry
M B
MB
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D015169:Colony Count, Microbial; D004926:Escherichia coli / Q000254:growth & development* / Q000276:immunology / Q000302:isolation & purification; D005260:Female; D005516:Food Microbiology; D006825:Hybridomas; D007124:Immunoenzyme Techniques; D008460:Meat; D051379:Mice
D000911:Antibodies, Monoclonal