Studies on the correlation between the presence of cytotoxic islet cell surface antibody and the incidence of overt diabetes in BB rats.
Antibodies directed to the surface of islet cells are often present in the sera of both humans with insulin-dependent diabetes and the BioBreeding (BB) rat. These autoantibodies have been implicated in the disease process although the correlation between the presence of islet cell surface antibodies (ICSA) and diabetes is controversial. In this study the presence of cytotoxic islet cell surface antibody (ICSA) was examined in 65 BB rats from 60-150 days of age. Blood was collected every 7-10 days and sera were assayed by a 51Cr-release assay using rat insulinoma cells (RINm5F) as target cells. Onset of diabetes was determined by bi-weekly urinalysis. The incidence of diabetes was 51%. While 29% of the rats exhibited cytotoxic ICSA and diabetes, 22% were overtly diabetic without showing any measurable ICSA. An additional 22% showed ICSA but did not become diabetic in the time period studied. These results suggest no correlation between cytotoxic ICSA and diabetes in our BB rat colony.
Tanguay
K E
KE
Department of Microbiology and Infectious Diseases, Faculty of Medicine, University of Calgary Health Sciences Centre, Alberta, Canada.
Yoon
J W
JW
Hart
D A
DA
D000818:Animals; D001323:Autoantibodies / Q000032:analysis*; D002460:Cell Line; D002462:Cell Membrane / Q000276:immunology; D003602:Cytotoxicity, Immunologic; D003921:Diabetes Mellitus, Experimental / Q000235:genetics / Q000276:immunology*; D005260:Female; D007340:Insulinoma; D007515:Islets of Langerhans / Q000276:immunology*; D008297:Male; D010190:Pancreatic Neoplasms; D051381:Rats; D011913:Rats, Inbred BB / Q000276:immunology*; D011919:Rats, Inbred Strains / Q000276:immunology*; D012737:Sex Factors
D001323:Autoantibodies; C024211:islet cell antibody