Transforming growth factor beta-1 in acute myocardial infarction in rats.
TGF-beta 1 has been examined in the heart during myocardial infarction caused by ligation of the left coronary artery. Infarcted and uninfarcted myocardium have been compared by immunohistochemical staining of TGF-beta 1 and by Northern blot analysis of mRNA. Normal ventricular myocytes are strongly stained by an antibody to TGF-beta 1. Progressive loss of staining of these myocytes begins within 1 hr after coronary ligation. However, by 24-48 hr after ligation, intense staining of myocytes at the margin of infarcted areas is seen. Northern blots of infarcted myocardium 48 hr after ligation show a 3- to 4-fold increase in the principal 2.4 kb TGF-beta 1 mRNA; there is also a marked increase in a minor 1.9 kb transcript. In the same tissue samples, there is a 2-fold decrease in the mRNA for the glycolytic enzyme, glyceraldehyde-3-phosphate dehydrogenase. The results indicate a significant role for TGF-beta in the response of the heart to injury.
Thompson
N L
NL
Laboratory of Chemoprevention, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892.
Bazoberry
F
F
Speir
E H
EH
Casscells
W
W
Ferrans
V J
VJ
Flanders
K C
KC
Kondaiah
P
P
Geiser
A G
AG
Sporn
M B
MB
D000818:Animals; D015152:Blotting, Northern; D003250:Constriction; D004195:Disease Models, Animal; D007124:Immunoenzyme Techniques; D008297:Male; D009203:Myocardial Infarction / Q000378:metabolism* / Q000473:pathology; D009206:Myocardium / Q000166:cytology; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D015291:Transforming Growth Factors / Q000235:genetics / Q000378:metabolism* / Q000502:physiology
D012333:RNA, Messenger; D015291:Transforming Growth Factors