Wall mannoproteins of the yeast and mycelial cells of Candida albicans: nature of the glycosidic bonds and polydispersity of their mannan moieties.
Zymolyase released between 20 and 25% of the total protein from purified walls of yeast (Y) and mycelial (M) cells of Candida albicans. The material released contained 92% carbohydrate (86% mannose and 6% glucose) and 7% protein. Over 85% of the carbohydrate was N-glycosidically linked to the protein and the rest (less than 15%) was linked O-glycosidically. Highly polydisperse, high molecular mass mannoproteins, resolved by electrophoresis as four defined bands in Y cells and two bands in M cells, had both types of sugar chains. A 34 kDa species found in both types of cells had a single 2.5 kDa N-glycosidically linked sugar chain and a 31.5 kDa protein moiety. Polydispersity in the high molecular mass mannoproteins was due to the N-linked sugar chains (mannan) with a molecular mass between 500 kDa and 20 kDa (average 100 kDa) in Y cells and between 400 kDa and 20 kDa (average 50 kDa) in M cells. Three mannoproteins of 34, 30 and 29 kDa secreted by protoplasts were associated with the high molecular mass mannoproteins, suggesting that this type of interaction might be related to the regeneration of the cell wall.
Elorza
M V
MV
Department de Microbiologia, Facultat de Farmacia, Universitat de València, Spain.
Marcilla
A
A
Sentandreu
R
R
D002176:Candida albicans / Q000166:cytology* / Q000378:metabolism; D002473:Cell Wall / Q000378:metabolism; D002850:Chromatography, Gel; D005656:Fungal Proteins / Q000378:metabolism*; D006027:Glycosides / Q000378:metabolism; D006867:Hydrolases / Q000378:metabolism; D008351:Mannans / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000378:metabolism*; D011523:Protoplasts / Q000378:metabolism
D005656:Fungal Proteins; D006027:Glycosides; D008351:Mannans; D008562:Membrane Glycoproteins; C025363:mannoproteins; C037833:zymolyase; D006867:Hydrolases