title: Purification of heat-labile enterotoxin from an enterotoxin from an enterotoxigenic Escherichia coli of human origin by monoclonal immunoaffinity chromatography.
abstract: Heat-labile enterotoxin (LT) was purified from an enterotoxigenic Escherichia coli 015H11 of human origin. The purification steps included French pressure cell disruption of the bacteria, salting-out, DEAE-Sephacel on chromatography. Application of this procedure resulted in a 95.1-fold purification of LT with a yield of 19.9% as determined by rabbit ileal loop assay. The final LT preparation showed only one protein-staining band on polyacrylamide gel electrophoresis, indicating that the purified LT was homogeneous.
authors.affiliation: Department of Microbiology and Biochemistry, College of Medicine, Seoul National University, Korea.
authors.forename: M J
authors.initials: MJ
authors.lastname: Cho
authors.forename: W H
authors.initials: WH
authors.lastname: Chang
authors.forename: M S
authors.initials: MS
authors.lastname: Choi
authors.forename: I S
authors.initials: IS
authors.lastname: Kim
authors.forename: J S
authors.initials: JS
authors.lastname: Kang
authors.forename: K H
authors.initials: KH
authors.lastname: Park
authors.forename: H K
authors.initials: HK
authors.lastname: Kim
authors.forename: C Y
authors.initials: CY
authors.lastname: Cha
authors.forename: H K
authors.initials: HK
authors.lastname: Chung
authors.forename: K H
authors.initials: KH
authors.lastname: Rhee
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000302:isolation & purification; D002846:Chromatography, Affinity / Q000379:methods; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D004768:Enterotoxins / Q000302:isolation & purification*; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000032:analysis* / Q000472:pathogenicity; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D011817:Rabbits
chemical_list: D000911:Antibodies, Monoclonal; D004768:Enterotoxins