Inquiries into the cloning and expression of the tuf gene of Thermus thermophilus HB8.
The tuf gene, which encodes the elongation factor Tu (EF-Tu), of Thermus thermophilus HB8 was cloned and sequenced. The whole G + C content of the tuf gene was 64.9%, and 84.5% of the third base in codon usage was either G or C, in which G was much favorable than C. The tuf gene was expressed in Escherichia coli under the control of the E. coli trp promoter. For the highly efficient expression, the SD sequence of the E. coli trpL was used instead of that of T. thermophilus HB8 and the length between the SD sequence and the initiation codon was controlled to keep 9 nucleotides.
Kushiro
A
A
Faculty of Pharmaceutical Sciences, Osaka University, Japan.
Shimizu
M
M
Ohyama
A
A
Tomita
K
K
D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods; D004926:Escherichia coli / Q000235:genetics; D005796:Genes; D005798:Genes, Bacterial; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D010444:Peptide Elongation Factor Tu / Q000235:genetics*; D011401:Promoter Regions, Genetic; D013824:Thermus / Q000235:genetics*; D014158:Transcription, Genetic
D010444:Peptide Elongation Factor Tu