An ELISA test for detecting chloroquine in urine.
Two ELISA tests for detecting chloroquine in urine have been developed using polyclonal and monoclonal antibodies which react with the 7-chloro-4-amino-quinoline part of the chloroquine molecule and thus recognize chloroquine, its metabolites, and amodiaquine. The ELISAs were sensitive and specific and did not cross-react with other commonly used antimalarials. In a field trial the chloroquine ELISA performed better than the Dill Glazko or Haskins colorimetric tests. A small proportion of urines gave an apparently false positive reaction when tested at a dilution of 1:10, but not when tested at higher dilution.
Shenton
F C
FC
Medical Research Council Laboratories, Fajara, Banjul, The Gambia.
Bots
M
M
Menon
A
A
Eggelte
T A
TA
de Wit
M
M
Greenwood
B M
BM
D000634:Aminoquinolines / Q000032:analysis; D000911:Antibodies, Monoclonal; D002675:Child, Preschool; D002738:Chloroquine / Q000652:urine*; D003124:Colorimetry; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D012680:Sensitivity and Specificity
D000634:Aminoquinolines; D000911:Antibodies, Monoclonal; C047186:7-chloro-4-aminoquinoline; D002738:Chloroquine