Development of DNA probes for Candida albicans.
An attempt was made to produce DNA probes that could be used as a rapid and efficient means of detecting candidiasis (invasive Candida infection) in immunocompromised patients. Whole DNA from Candida albicans was digested with restriction endonuclease, and the resulting fragments were randomly cloned into a plasmid vector. Several recombinant plasmids were evaluated for cross-hybridization to various other Candida species, other fungal DNAs, and to nonfungal DNAs. Cross reactions were observed between the probes and different yeasts, but none with unrelated DNAs. Some recombinants were genus-specific, and two of these were applied to the analysis of C. albicans growth curves. It became evident that, although both 32P- and biotin-labelled probes could be made quite sensitive, a possible limitation in their diagnostic potential was the poor liberation of Candida DNA from cells. Thus, better methods of treatment of clinical specimens will be required before such probes will be useful in routine diagnosis.
Cheung
L L
LL
Division of Medical Microbiology, University of British Columbia, Vancouver, Canada.
Hudson
J B
JB
D001345:Autoradiography; D002176:Candida albicans / Q000235:genetics; D002177:Candidiasis / Q000175:diagnosis*; D003429:Cross Reactions; D015342:DNA Probes; D004271:DNA, Fungal / Q000032:analysis / Q000302:isolation & purification; D010761:Phosphorus Radioisotopes
D015342:DNA Probes; D004271:DNA, Fungal; D010761:Phosphorus Radioisotopes