In vitro inhibition of mouse epidermal cell lipoxygenase by flavonoids: structure-activity relationships.
Lipoxygenase was extracted from mouse epidermal cells and partially purified by ammonium sulfate precipitation. Using a biological oxygen monitor for activity measurements and linoleic acid as substrate, the effect of a series of flavonoids on lipoxygenase activity was determined. Flavone itself did not inhibit lipoxygenase, but hydroxylated derivatives retaining the double bond in the 2,3 position and having a hydroxyl group in the 3 position (such as quercetin and 3-hydroxy-flavone) were potent lipoxygenase inhibitors compared to flavonoids without those particular functional groups.
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D000818:Animals; D000831:Animals, Newborn; D005419:Flavonoids / Q000494:pharmacology*; D007700:Kinetics; D008084:Lipoxygenase / Q000302:isolation & purification; D016859:Lipoxygenase Inhibitors; D051379:Mice; D012867:Skin / Q000201:enzymology*; D013329:Structure-Activity Relationship
D005419:Flavonoids; D016859:Lipoxygenase Inhibitors; D008084:Lipoxygenase