Immunological study of glycolipoprotein isolated from extracellular slime of different Pseudomonas aeruginosa serogroups.
Glycolipoprotein (GLP) was isolated by water-phenol extraction and ultracentrifugation from crude extracellular slime (C-ES) of 14 strains of Pseudomonas aeruginosa. The yields of GLP obtained between different methods were 65.6 +/- 8.4% and 46.4 +/- 11.2% of the dry weight of all C-ES fractions. GLP stimulated active immunity in mice against challenge with different immunotypes (or serogroups) of P. aeruginosa. Phenol fractions (PFr) of different P. aeruginosa strains behaved similarly. As compared to the corresponding C-ES in active mouse protection test, the GLP of several strains was less active, whereas that of some other strains was more protective.
D000818:Animals; D001426:Bacterial Proteins; D006023:Glycoproteins / Q000032:analysis / Q000276:immunology* / Q000302:isolation & purification; D006801:Humans; D007110:Immunity, Active; D007114:Immunization; D008074:Lipoproteins / Q000032:analysis / Q000276:immunology* / Q000302:isolation & purification; D051379:Mice; D011552:Pseudomonas Infections / Q000276:immunology; D011550:Pseudomonas aeruginosa / Q000276:immunology*; D014461:Ultracentrifugation
D001426:Bacterial Proteins; D006023:Glycoproteins; D008074:Lipoproteins; C025431:extracellular slime proteins, bacterial