In vivo expression and regulation of murine IL 2 receptors after antigen sensitization.
We have studied the in vivo regulation and expression of the murine IL 2 receptor after antigen sensitization. High and low affinity receptor expression has been studied by flow cytometry analysis and radiolabeled IL 2 binding. Both anti-IL 2 receptor antibody and unlabeled IL 2 inhibited the radiolabeled IL 2 binding. The kinetics of expression of the IL 2 receptor closely correspond to the proliferative response, as assessed by IUdR radioisotope uptake. The expression of IL 2 receptors correlated with the proliferative response profile of the murine strains surveyed during a study of responses to picryl chloride. Neither immune compromised 4-mo-old MRL/lpr mice nor athymic nude mice generated significant antigen-specific proliferative responses or IL 2 receptor expression after antigen sensitization. Mice rendered specifically unresponsive to antigen displayed reduced proliferative responses to the tolerizing antigen, as well as reduced numbers of IL 2 receptor-positive cells. Finally, the treatment of mice with immunosuppressive drugs reduced both the proliferative responses as well as the number of IL 2 receptor-positive cells. However, at the cellular level, the drugs produced different effects on IL 2 receptor expression.
D000818:Animals; D003520:Cyclophosphamide / Q000494:pharmacology; D003524:Cyclosporins / Q000494:pharmacology; D003907:Dexamethasone / Q000494:pharmacology; D005434:Flow Cytometry; D006649:Histocompatibility Antigens / Q000276:immunology; D007108:Immune Tolerance; D007114:Immunization; D007165:Immunosuppression Therapy; D007376:Interleukin-2 / Q000378:metabolism*; D008198:Lymph Nodes / Q000276:immunology; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008815:Mice, Inbred Strains; D010853:Picryl Chloride / Q000276:immunology; D011971:Receptors, Immunologic / Q000378:metabolism*; D015375:Receptors, Interleukin-2; D013601:T-Lymphocytes / Q000276:immunology*; D013997:Time Factors
D003524:Cyclosporins; D006649:Histocompatibility Antigens; D007376:Interleukin-2; D011971:Receptors, Immunologic; D015375:Receptors, Interleukin-2; D003907:Dexamethasone; D003520:Cyclophosphamide; D010853:Picryl Chloride