title: The protein receptor for cholerabacteriophage phi 149.
abstract: Choleraphage phi 149 receptor activity was found in the outer membrane (OM) protein of Vibrio cholerae 154. Receptor protein for phage phi 149 was separated from trypsin-treated OM on a Sephadex G-100 column. Of the three peaks obtained, phage receptor activity was noted only in peak II. SDS-PAGE showed that the Mr of the protein was 35,000. The protein was heat-labile and protease-sensitive. The specificity of this protein as choleraphage phi 149 receptor was investigated by carrying out a protection experiment by anti-protein (peak II) rabbit sera.
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000032:analysis*; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006358:Hot Temperature; D005779:Immunodiffusion; D008970:Molecular Weight; D010447:Peptide Hydrolases / Q000378:metabolism; D011991:Receptors, Virus / Q000032:analysis*; D014357:Trypsin / Q000378:metabolism; D014734:Vibrio cholerae / Q000032:analysis*
chemical_list: D001425:Bacterial Outer Membrane Proteins; D011991:Receptors, Virus; C051888:choleraphage phi 149 receptor; D010447:Peptide Hydrolases; D014357:Trypsin