[Immunofluorescent reaction of antibodies directed against antigens from the intestinal epithelium of Schistosoma mansoni. III. Study of the reactivity of monoclonal antibodies].
A monoclonal mouse antibody of IgM class was raised against an epitope of the gut epithelium of the adult worm and was applied to the detection of antigen in parasite infection. The antigen was found in urine from mice and hamsters infected with Schistosoma mansoni; a good correlation between the concentration of antigen and worm burden was observed. The antigen was thermostable, soluble in trichloracetic acid; it was not hydrolysed by proteinase K but it was destroyed by metaperiodate. The antigen was shown to be Schistosoma genus specific. It was found in different developmental stages of the parasite. High levels were detected in egg extracts.
D000818:Animals; D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth / Q000032:analysis*; D000954:Antigens, Surface / Q000032:analysis*; D004848:Epithelium / Q000276:immunology; D005455:Fluorescent Antibody Technique; D007422:Intestines / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D012550:Schistosoma mansoni / Q000276:immunology*; D012555:Schistosomiasis mansoni / Q000175:diagnosis* / Q000276:immunology / Q000652:urine
D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth; D000954:Antigens, Surface
Etude par la réaction d'immunofluorescence des anticorps dirigés contre les antigènes de l'épithélium intestinal de Schistosoma mansoni III--Etude de la réactivité d'un anticorps monoclonal.