Serum amyloid P-component-induced enhancement of macrophage listericidal activity.
Purified serum amyloid P component (SAP), the major acute-phase reactant of mice, augmented the in vitro listericidal activity of inflammatory (elicited) macrophages, bone marrow-derived monocytes, and macrophages from a subcutaneous site of inflammation. Monocytes and macrophages from C57BL/B6 mice, which are relatively resistant to Listeria monocytogenes, exhibited a significantly greater enhanced killing capacity for listeria than macrophages from listeria-susceptible A/J mice. SAP did not alter the extent of phagocytosis by macrophages of opsonized L. monocytogenes, nor was SAP opsonic for listeria. Mannose-derived simple sugars inhibited the binding of SAP to macrophages and consequently prevented the enhanced SAP-dependent listericidal activity. Macrophages from lipopolysaccharide-hyporesponsive mice also had increased microbicidal activity following incubation with SAP. SAP activated macrophages independently of lymphokine. Therefore, SAP may serve as a mediator of the heightened nonspecific host defense response that is associated with the acute phase of the systemic inflammatory response.
D000682:Amyloid / Q000276:immunology*; D000818:Animals; D001770:Blood Bactericidal Activity; D001798:Blood Proteins / Q000276:immunology; D001854:Bone Marrow Cells; D002241:Carbohydrates / Q000276:immunology; D005260:Female; D008089:Listeria monocytogenes / Q000276:immunology*; D008088:Listeriosis / Q000276:immunology*; D008222:Lymphokines / Q000276:immunology; D008262:Macrophage Activation; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D008815:Mice, Inbred Strains; D010529:Peritoneal Cavity / Q000166:cytology; D010587:Phagocytosis; D000683:Serum Amyloid P-Component
D000682:Amyloid; D001798:Blood Proteins; D002241:Carbohydrates; D008222:Lymphokines; D000683:Serum Amyloid P-Component