[Acute toxicity test on dermal application of NT-1 tape and 10% nitroglycerin (NT-1 ointment)].
An acute toxicity of NT-1 tape was investigated in mice, rats and rabbits, and also an acute toxicity of NT-1 ointment in rabbits. An irritation of NT-1 tape was investigated in rabbits. Dead animals treated with NT-1 tape or ointment were not observed in reliable maximum administration dose of 0.88 mg/body as GTN (nitroglycerin) in mice (male; about 29.1 mg/kg, female; about 35.2 mg/kg), of 3.96 mg/body as GTN in rats (male; about 26.0 mg/kg, female; about 28.9 mg/kg) and of 480 mg/kg as GTN in rabbits. There were not toxic signs in mice and rats. The below findings were observed in rabbits treated mainly with NT-1 ointment: decreased spontaneous activity, decreased reactivity to various stimuli such as sound and touch, and behavior of binding food box. However, the rabbits recovered from those abnormal behavior in a day. Any body weight changes and any autopsy findings attributable to NT-1 tape or ointment were not observed in mice, rats and rabbits. The effects on ECG were not observed in rabbits. The GTN absorption ratio in rabbits treated with NT-1 ointment was thought to be 60-70% from GTN residual ratio. The skin irritation was not observed in rabbits treated with NT-1 tape.
D000279:Administration, Cutaneous; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D004562:Electrocardiography; D005260:Female; D007509:Irritants; D008297:Male; D051379:Mice; D005996:Nitroglycerin / Q000008:administration & dosage / Q000378:metabolism / Q000633:toxicity*; D009824:Ointments; D011817:Rabbits; D051381:Rats; D012867:Skin / Q000187:drug effects*; D012869:Skin Absorption
D007509:Irritants; D009824:Ointments; D005996:Nitroglycerin