title: In vivo clearance of antibody-sensitized human drug carrier erythrocytes.
abstract: Antibody coating of resealed drug carrier erythrocytes may be useful for drug targeting to the reticuloendothelial system. We have investigated the survival in the circulation of anti-Rh antibody (IgG anti-D)-coated autologous erythrocytes loaded with gentamicin by hypoosmotic dialysis. Five subjects were injected with 15.2 +/- 0.4 ml and five additional subjects with 62.8 +/- 1.5 ml carrier cells. Survival of the cells was monitored by intraerythrocytic gentamicin concentration in blood. In the first subject group initial t1/2 was 0.21 +/- 0.06 hours and terminal t1/2 was 1.71 +/- 0.36 hours. In the second group initial t1/2 was 0.59 +/- 0.21 hours followed by a slow phase with a t1/2 of 89 +/- 28 hours. Results indicate that rapid drug delivery to the reticuloendothelial system by antibody-sensitized carrier erythrocytes is possible, but small volumes of erythrocytes seem more efficient.
mesh_terms: D000328:Adult; D000906:Antibodies; D004304:Dosage Forms; D004912:Erythrocytes / Q000378:metabolism*; D005839:Gentamicins / Q000097:blood; D006801:Humans; D007700:Kinetics; D008297:Male; D012157:Mononuclear Phagocyte System / Q000187:drug effects; D012204:Rh-Hr Blood-Group System / Q000276:immunology*
chemical_list: D000906:Antibodies; D004304:Dosage Forms; D005839:Gentamicins; D012204:Rh-Hr Blood-Group System