Measurement of micronuclei by cytokinesis-block method in cultured Chinese hamster cells: comparison with types and rates of chromosome aberrations.
The cytokinesis-block method of Fenech and Morley (1985) has been tested for the enumeration and characterization of micronuclei in exponentially growing Chinese hamster cells in culture. The consistent dose-response relations were obtained in cultures treated with mitomycin C, caffeine and colcemid. Comparison with the chromosome aberration frequencies indicated that approximately 30% of the acentric chromosomes are expressed as micronuclei in the mitomycin C and caffeine treated cells. The size distribution of the micronuclei suggested that the base-line frequency of micronuclei is mainly a reflection of mitotic dysfunctions rather than chromosome structural aberrations.
D000818:Animals; D002110:Caffeine / Q000633:toxicity; D002455:Cell Division; D002460:Cell Line; D002467:Cell Nucleus / Q000648:ultrastructure*; D002869:Chromosome Aberrations; D006224:Cricetinae; D003412:Cricetulus; D003571:Cytochalasin B / Q000633:toxicity; D003703:Demecolcine / Q000633:toxicity; D016685:Mitomycin; D008937:Mitomycins / Q000633:toxicity; D009152:Mutagenicity Tests
D008937:Mitomycins; D003571:Cytochalasin B; D002110:Caffeine; D016685:Mitomycin; D003703:Demecolcine