Botulinum neurotoxin inhibits depolarization-stimulated protein phosphorylation in pure cholinergic synaptosomes.
Botulinum neurotoxin, a strong blocker of acetylcholine release at peripheral cholinergic synapses, inhibits depolarization-stimulated protein phosphorylation in pure cholinergic synaptosomes isolated from the electric organ of Torpedo marmorata. Moreover, tetrodotoxin has the same effect on protein phosphorylation when cholinergic synaptosomes are depolarized by veratridine. Correlation between presynaptic protein phosphorylation and acetylcholine release is suggested by the fact that botulinum neurotoxin blocks specifically neurotransmitter release without affecting membrane depolarization and calcium fluxes in our synaptosomal preparation.
D000818:Animals; D001905:Botulinum Toxins / Q000494:pharmacology*; D000001:Calcimycin / Q000494:pharmacology; D004557:Electric Organ / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D066298:In Vitro Techniques; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009498:Neurotoxins / Q000494:pharmacology*; D010275:Parasympathetic Nervous System / Q000378:metabolism*; D010766:Phosphorylation; D011188:Potassium / Q000494:pharmacology; D013574:Synaptosomes / Q000378:metabolism*; D013779:Tetrodotoxin / Q000494:pharmacology; D014101:Torpedo; D014701:Veratridine / Q000494:pharmacology
D009419:Nerve Tissue Proteins; D009498:Neurotoxins; D000001:Calcimycin; D013779:Tetrodotoxin; D014701:Veratridine; D001905:Botulinum Toxins; D011188:Potassium