Rapid screening method for monoclonal antibodies against cytoskeletal proteins.
A horseradish peroxidase/anti-horseradish peroxidase monoclonal mouse antibody complex was prepared and used for the detection of mouse monoclonal antibodies against proteins of the whole cytoskeleton. A rapid qualitative assay of large number of antibody samples on the whole cytoskeleton and an early determination of their specificity by the immunoblot technique is described.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis*; D000918:Antibody Specificity; D002460:Cell Line; D003598:Cytoskeletal Proteins / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D005347:Fibroblasts; D005455:Fluorescent Antibody Technique; D006801:Humans; D007124:Immunoenzyme Techniques; D007158:Immunologic Techniques; D051379:Mice
D000911:Antibodies, Monoclonal; D003598:Cytoskeletal Proteins