Expression of the M-CSF (CSF-1) gene by human monocytes.
Monocyte colony-stimulating factor (M-CSF, CSF-1) is a macrophage lineage-specific growth factor. Northern blot analysis using a human M-CSF cDNA probe and a specific bioassay for human M-CSF were used to investigate the cellular sources of M-CSF. Expression of the M-CSF gene was induced in blood mononuclear cells stimulated by phorbol myristate acetate (PMA) or gamma-interferon. When mononuclear cells were fractionated into highly purified populations of T cells and monocytes, M-CSF transcripts were detected predominantly in monocytes. Further, monocytes stimulated with PMA released an M-CSF-like activity. These results demonstrate that M-CSF can be produced by activated cells of the macrophage lineage.
D002478:Cells, Cultured; D003101:Collodion; D003115:Colony-Stimulating Factors / Q000235:genetics* / Q000378:metabolism; D004247:DNA; D004591:Electrophoresis, Polyacrylamide Gel; D006133:Growth Substances / Q000378:metabolism; D006412:Hematopoietic Stem Cells / Q000378:metabolism; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D008262:Macrophage Activation; D009000:Monocytes / Q000502:physiology*; D013601:T-Lymphocytes / Q000187:drug effects / Q000502:physiology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014158:Transcription, Genetic
D003115:Colony-Stimulating Factors; D006133:Growth Substances; D007371:Interferon-gamma; D003101:Collodion; D004247:DNA; D013755:Tetradecanoylphorbol Acetate