Effect of particle size of activated charcoal on separation of Triton X-100 from protein, liver cytosol, and lipoxygenase extracts.
Triton X-100 was almost completely removed from bovine serum albumin solutions, BALB/c mouse liver extracts, and avocado peel lipoxygenase extracts by stirring the samples for 30 min in the presence of 250-350 mesh activated charcoal. The procedure did not remove protein significantly and did not reduce enzyme activity. At higher charcoal particle sizes, the efficiency of Triton adsorption was decreased and protein adsorption was increased. ElevateD temperatures enhanced Triton and protein adsorption. Adsorption on activated charcoal of 250-350 mesh is a simple and rapid procedure for Triton removal at a ratio of 0.23 g Triton X-100 per gram of activated charcoal.
D000818:Animals; D002606:Charcoal; D003600:Cytosol / Q000032:analysis; D008084:Lipoxygenase / Q000032:analysis*; D008099:Liver / Q000032:analysis*; D051379:Mice; D008807:Mice, Inbred BALB C; D017830:Octoxynol; D010316:Particle Size; D011092:Polyethylene Glycols / Q000302:isolation & purification*; D011506:Proteins / Q000302:isolation & purification*; D012710:Serum Albumin, Bovine / Q000032:analysis; D013696:Temperature
D011506:Proteins; D002606:Charcoal; D012710:Serum Albumin, Bovine; D011092:Polyethylene Glycols; D017830:Octoxynol; D008084:Lipoxygenase