title: Transcripts of functionally rearranged gamma genes in primary T cells of adult immunocompetent mice.
abstract: The T-cell specific, rearranging gamma-chain genes bear striking resemblance to T-cell receptor and immunoglobulin genes, but the role of gamma remains unknown. A central problem is to understand the conditions under which gamma RNA is expressed in cells. The transcription of gamma is abundant in T cells of fetal thymi, but is negligible in peripheral T cells of adults, suggesting that gamma is involved in development of the T-cell repertoire. However, gamma RNA was originally cloned from established lines of cytotoxic T cells (CTLs) derived from adult mice and this expression has been ascribed to non-physiological cell growth. Possibly consistent with this, most of the gamma RNA derives from genes rearranged abortively at the V gamma-J gamma junction of immunoglobulin genes, where V is the variable segment and J the joint segment. Here, we report the detailed analysis of gamma transcription in T cells of adult mice, and find that transcription may occur in T cells with a broad range of surface phenotypes; that it is predominantly of a single V gamma-C gamma unit (where C is the constant region); and that in cells freshly explanted from animals it can be of productively rearranged genes.
mesh_terms: D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface / Q000032:analysis; D004247:DNA / Q000235:genetics; D005434:Flow Cytometry; D005786:Gene Expression Regulation; D005796:Genes; D007142:Immunoglobulin gamma-Chains / Q000235:genetics; D051379:Mice; D011519:Proto-Oncogenes; D012333:RNA, Messenger / Q000235:genetics; D011948:Receptors, Antigen, T-Cell / Q000235:genetics; D011995:Recombination, Genetic; D013601:T-Lymphocytes / Q000502:physiology*; D013602:T-Lymphocytes, Cytotoxic / Q000502:physiology
chemical_list: D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface; D007142:Immunoglobulin gamma-Chains; D012333:RNA, Messenger; D011948:Receptors, Antigen, T-Cell; D004247:DNA