Isolation and properties of the complex nonheme-iron-containing cytochrome b557 (bacterioferritin) from Pseudomonas aeruginosa.
A nonheme-iron-containing cytochrome b557, also known as bacterioferritin, was isolated from Pseudomonas aeruginosa. Gel electrophoresis showed that the protein subunits had a molecular weight of 18 kD and it is suggested that the intact molecule contained 24 subunits. The isolated protein contained 8.7% by weight Fe and 8% by weight phosphate. Most of the Fe was contained in the nonheme-iron core and could be readily removed by dialysis against 0.12 M thioglycollic acid. The resulting apobacterioferritin contained approximately one protoporphyrin IX group for every five subunits and, in addition, an unidentified fluorophor. The nonheme-iron core was found to be amorphous with a mean core size of 60-65 A.
D001426:Bacterial Proteins; D003573:Cytochrome b Group / Q000302:isolation & purification* / Q000378:metabolism; D005293:Ferritins; D007501:Iron / Q000032:analysis; D007700:Kinetics; D011550:Pseudomonas aeruginosa / Q000378:metabolism*; D013050:Spectrometry, Fluorescence
D001426:Bacterial Proteins; D003573:Cytochrome b Group; D005293:Ferritins; C018032:bacterioferritin; D007501:Iron