Thrombin purification by one-step preparative affinity chromatography on modified polystyrenes.
Insoluble polystyrenes substituted with sulphonate and L-arginyl methyl ester have been synthesized. Using their specific affinity for thrombin, we developed a simple one-step chromatographic procedure for thrombin purification. As a control, insoluble polystyrenes substituted only with sulphonate groups were tested. The results obtained confirmed the importance of the arginyl residues grafted onto these polymers to obtain an affinity matrix useful for purifying thrombin with a high specific activity and a good recovery.
D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D005779:Immunodiffusion; D011137:Polystyrenes / Q000032:analysis*; D012116:Resins, Plant; D012967:Sodium Dodecyl Sulfate; D013917:Thrombin / Q000302:isolation & purification*
D011137:Polystyrenes; D012116:Resins, Plant; D012967:Sodium Dodecyl Sulfate; D013917:Thrombin