Characterization of the human syncytiotrophoblast plasma membrane associated components.
A purification procedure of synctiotrophoblast plasma membrane (STPM) has previously been described. We now report a further characterization of their associated components. By immunochemical analysis two groups of proteins were found. The first fraction solubilized by chaotrope treatment was made up with a complex of IgG, albumin, transferrin and alpha-fetoprotein. The second fraction, insolubilized by KCl-treatment but by detergents, did not react with antisera to human proteins; it contained human chorionic gonadotropin and placental lactogen. The amino acid, carbohydrate and lipid content of STPM were determined. High galactosyl- and sialyl-transferase activities were found in purified STPM. The role of these various components on the acceptance of the fetal allograft is discussed.
D000596:Amino Acids / Q000032:analysis; D002241:Carbohydrates / Q000032:analysis; D002458:Cell Fractionation / Q000379:methods; D002462:Cell Membrane / Q000648:ultrastructure; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006728:Hormones / Q000032:analysis; D006801:Humans; D005779:Immunodiffusion; D011247:Pregnancy; D011506:Proteins / Q000032:analysis*; D013256:Steroids / Q000032:analysis; D014327:Trophoblasts / Q000648:ultrastructure*
D000596:Amino Acids; D002241:Carbohydrates; D006728:Hormones; D011506:Proteins; D013256:Steroids