Some chemical properties of tissue plasminogen activator purified from paranasal mucous membrane.
Plasminogen activator (PA) was purified from an acetone powder preparation of paranasal mucous membrane with chronic sinusitis, and some chemical properties of the purified PA were investigated in this paper. Zn-imminodiacetate affinity chromatography, lysine sepharose affinity chromatography and ultrafiltration for concentrating a PA fraction were consecutively performed to purify the PA from the acetone powder preparation. Finally, gel filtration was performed using Sephacryl S-200 in order to estimate the molecular weight of the purified PA. The purified PA in this experiment showed a stronger affinity to fibrin than urokinase did. The molecular weight of the purified PA was estimated to be 65,000 to 70,000 daltons as determined by Sephacryl S-200 gel filtration. The Km of the purified PA was 0.11 mM. From these results, it is apparent that the PA purified from an acetone powder preparation of paranasal mucous membrane belongs to the class of tissue type plasminogen activators (t-PA).
D002908:Chronic Disease; D006801:Humans; D008970:Molecular Weight; D009297:Nasal Mucosa / Q000378:metabolism*; D010256:Paranasal Sinuses / Q000378:metabolism*; D012852:Sinusitis / Q000378:metabolism*; D010959:Tissue Plasminogen Activator / Q000302:isolation & purification*
D010959:Tissue Plasminogen Activator