title: Synthesis of dolichol derivatives in trypanosomatids. Characterization of enzymatic patterns.
abstract: We have previously described that in certain parasitic protozoa, namely the trypanosomatids, the dolichol-P-P-linked oligosaccharides synthesized in vivo and transferred to protein are devoid of glucose residues and contain 6, 7, or 9 mannose units depending on the species. We have now conducted a cell-free characterization of the enzymatic patterns responsible for these phenotypes. Microsomes from Trypanosoma cruzi, Crithidia fasciculata, Leishmania enriettii, and Blastocrithidia culicis were found to synthesize dolichol-P-[14C]Man but not dolichol-P-[14C]Glc when incubated with rat liver dolichol-P and GDP-[14C]Man or UDP-[14C]Glc, thus providing for an explanation to the absence of glucosylated dolichol-P-P derivatives. Formation of dolichol-P-P-oligosaccharides was assayed in incubation mixtures containing rat liver dolichol-P, GDP-[14C]Man, microsomes, and unlabeled Man5-8GlcNAc2-P-P-dolichol from bovine liver. Membranes from species synthesizing dolichol-P-P-linked Man6GlcNAc2 or Man7GlcNAc2 in vivo were found to synthesize the same compounds but not the higher homologues in the cell-free assay. Species forming Man9GlcNAc2-P-P-dolichol in vivo were found to synthesize lipid-linked Man7GlcNAc2, Man8GlcNAc2, and Man9GlcNAc2 in vitro. It is concluded that there are at least three and probably four different dolichol-P-Man-dependent enzymatic activities involved in the synthesis of dolichol-P-P-linked Man9GlcNAc2 and that microorganisms not forming that compound are devoid of all mannosyltransferases responsible for the addition of the missing residues and not only of the enzyme involved in the synthesis of the homologue higher than the oligosaccharide occurring in vivo by a single mannose unit.
mesh_terms: D000818:Animals; D002417:Cattle; D003421:Crithidia / Q000201:enzymology; D004187:Disaccharides / Q000378:metabolism; D004287:Dolichol Monophosphate Mannose / Q000378:metabolism; D004288:Dolichol Phosphates / Q000096:biosynthesis*; D007891:Leishmania / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D008861:Microsomes / Q000201:enzymology; D008862:Microsomes, Liver / Q000201:enzymology; D011103:Polyisoprenyl Phosphate Monosaccharides / Q000378:metabolism; D011106:Polyisoprenyl Phosphates / Q000096:biosynthesis*; D051381:Rats; D014349:Trypanosoma cruzi / Q000201:enzymology; D014351:Trypanosomatina / Q000201:enzymology*
chemical_list: D004187:Disaccharides; D004288:Dolichol Phosphates; D011103:Polyisoprenyl Phosphate Monosaccharides; D011106:Polyisoprenyl Phosphates; D004287:Dolichol Monophosphate Mannose; C035093:4-O-mannopyranosyl-2-acetamido-2-deoxyglucose; C018439:N-acetylglucosaminylpyrophosphoryldolichol