Heterogeneous glycosylation of the EXG1 gene product accounts for the two extracellular exo-beta-glucanases of Saccharomyces cerevisiae.
Two exo-beta-glucanases of glycoprotein nature can be detected in culture supernatants of Saccharomyces cerevisiae cells. These exo-beta-glucanases show different Mr values and kinetic properties, although they are immunologically related. Their carbohydrate content and the electrophoretic mobility of both endoglycosidase H-treated exo-beta-glucanases suggest that they share the same protein fraction. Studies at genetic level relate the production of both extracellular exo-beta-glucanases with the expression of a single-copy gene in S. cerevisiae. Expression of this gene in another yeast, Schizosaccharomyces pombe, demonstrates that it codes for a protein with exo-beta-glucanase activity whose heterogeneous N-glycosylation accounts for both extracellular exo-beta-glucanases of S. cerevisiae.
D002241:Carbohydrates / Q000032:analysis; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000032:analysis / Q000235:genetics; D005796:Genes; D005815:Genetic Code; D044643:Glucan 1,4-beta-Glucosidase; D005959:Glucosidases / Q000235:genetics*; D006023:Glycoproteins / Q000032:analysis*; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D001617:beta-Glucosidase / Q000235:genetics*
D002241:Carbohydrates; D003470:Culture Media; D005656:Fungal Proteins; D006023:Glycoproteins; D005959:Glucosidases; D001617:beta-Glucosidase; D044643:Glucan 1,4-beta-Glucosidase