title: Electron microscopic visualization of the capsule of Pasteurella multocida.
abstract: The results of electron microscopic visualization of capsules of Pasteurella multocida by means of negative staining and section methods are described. Using ruthenium red or a complex of platinum in negative staining, relatively homogeneous capsules were visualized, using alcian-blue, coarse-fibrous structured capsules appeared. The incubation with antiserum partially revealed narrow capsule zones in the sections. Ferritin-linked IgG labelled amorphous material between the bacteria or floccular appendages on the bacterial surface. Gold-linked Con A deposited on thick cord structures, on loose material between the bacteria and sometimes on their cell wall.
mesh_terms: D002473:Cell Wall / Q000648:ultrastructure; D004396:Coloring Agents; D007106:Immune Sera; D008854:Microscopy, Electron; D010325:Pasteurella / Q000648:ultrastructure*
chemical_list: D004396:Coloring Agents; D007106:Immune Sera