Dual-column determination of albumin and immunoglobulin G in serum by high-performance affinity chromatography.
High-performance affinity chromatography was used for the simultaneous determination of albumin and immunoglobulin G in serum. Two columns in series, the first containing immobilized anti-albumin antibodies and the other containing protein A for binding immunoglobulin G, were eluted separately at pH 3 by means of a column-switching system. This method gave results in good agreement with commercially available methods, while requiring only 2 microliter of serum and 6.0 min per cycle. It was shown that albumin and immunoglobulin G were selectively retained, with little interference from other components, including immunoglobulins A and M.
D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D006863:Hydrogen-Ion Concentration; D005779:Immunodiffusion; D007074:Immunoglobulin G / Q000032:analysis*; D007202:Indicators and Reagents; D007700:Kinetics; D012709:Serum Albumin / Q000032:analysis*; D013205:Staphylococcal Protein A / Q000032:analysis
D007074:Immunoglobulin G; D007202:Indicators and Reagents; D012709:Serum Albumin; D013205:Staphylococcal Protein A