Meta-vinculin distribution in adult human tissues and cultured cells.
Meta-vinculin distribution in adult human tissue was studied by immunoblotting technique. Meta-vinculin was found in smooth (aorta wall and myometrium) and cardiac muscle, rather than in skeletal muscle, liver, kidney and cultured cells - macrophages, foreskin fibroblasts, peripheral blood lymphocytes and vascular endothelial cells. In the primary culture of smooth muscle cells from human aorta the meta-vinculin/vinculin ratio was reduced, and on the onset of cell division meta-vinculin could hardly be detected. Subcultured smooth muscle cells from human aorta did not contain meta-vinculin. The data show that the presence of meta-vinculin is characteristic of 'contractile' smooth muscle cells rather than of proliferating in vitro.
D001011:Aorta / Q000032:analysis; D002478:Cells, Cultured; D006801:Humans; D009124:Muscle Proteins / Q000032:analysis*; D009130:Muscle, Smooth / Q000032:analysis; D009206:Myocardium / Q000032:analysis; D016596:Vinculin
D009124:Muscle Proteins; C488955:VCL protein, human; D016596:Vinculin