Resistance to oxidative stress by hyperplastic and neoplastic rat liver tissue monitored in terms of production of unpolar and medium polar carbonyls.
The susceptibility of rat liver tissue to oxidative stress during its neoplastic transformation was analyzed by both qualitative and quantitative measurements of the carbonyl products of lipid peroxidation. Diethylnitrosamine was used as initiating agent of hepatocarcinogenesis and lipid peroxidation levels were monitored in the homogenates from normal liver, hyperplastic nodules and tumour, incubated in the presence or in the absence of ascorbate or adenosine diphosphate-iron complex. While the basal levels of lipid peroxidation in the three experimental conditions were found to be quite similar, in the presence of the pro-oxidant stimulus a remarkable reduction in aldehyde production was shown not only by the hepatoma tissue but also by the preneoplastic nodules.
D000447:Aldehydes / Q000378:metabolism; D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000032:analysis; D002471:Cell Transformation, Neoplastic; D002851:Chromatography, High Pressure Liquid; D004052:Diethylnitrosamine; D005227:Fatty Acids / Q000032:analysis; D006965:Hyperplasia; D008054:Lipid Peroxides / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008113:Liver Neoplasms / Q000378:metabolism*; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D010084:Oxidation-Reduction; D010714:Phosphatidylethanolamines / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains
D000447:Aldehydes; D001095:Arachidonic Acids; D005227:Fatty Acids; D008054:Lipid Peroxides; D010714:Phosphatidylethanolamines; D016718:Arachidonic Acid; D004052:Diethylnitrosamine; D008315:Malondialdehyde; C027576:4-hydroxy-2-nonenal