Hydrophobic adsorbents for the isolation and purification of biosynthetic human growth hormone from crude fermentation mixtures.
Hydrophobic interaction chromatography was used as the initial step for the concentration and partial purification of biosynthetic human growth hormone (hGH). This molecule was extracted from the culture medium of a transformed monkey kidney cell line by batch adsorption either to Octyl-Sepharose or Phenyl-Sepharose. The adsorbate was decanted and packed into a column for elution. This initial work-up procedure rendered a concentrated hGH solution that was further purified by ion-exchange and gel filtration chromatography. The hGH obtained was homogeneous, based on polyacrylamide gel electrophoresis, N-terminal sequence and fingerprint analysis.
D000327:Adsorption; D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D002852:Chromatography, Ion Exchange; D005285:Fermentation; D013006:Growth Hormone / Q000096:biosynthesis / Q000302:isolation & purification*; D000882:Haplorhini; D006801:Humans; D007668:Kidney / Q000166:cytology; D011994:Recombinant Proteins
D011994:Recombinant Proteins; D013006:Growth Hormone