Phosphorylation by protein kinase C of a synthetic heptapeptide bearing a lysine residue on the C terminal side of serine.
A peptide, Ala-Ser-Gly-Ser-Phe-Lys-Leu, which corresponds to Ala103-Leu109 of Hl histone, was synthesized and tested as substrate for protein kinase C. The serine residue at position 4 was phosphorylated specifically. Another peptide lacking the lysine at position 6 was not phosphorylated by the same enzyme, indicating the importance of that basic residue as the recognition site for protein kinase C.
D000595:Amino Acid Sequence; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D008055:Lipids / Q000494:pharmacology; D008239:Lysine; D009842:Oligopeptides / Q000378:metabolism; D010766:Phosphorylation; D011493:Protein Kinase C / Q000378:metabolism*; D012694:Serine; D013329:Structure-Activity Relationship; D013379:Substrate Specificity; D013552:Swine
D008055:Lipids; D009842:Oligopeptides; D012694:Serine; D011493:Protein Kinase C; D008239:Lysine; D002118:Calcium