Cathepsins B and D in rat brain glia during experimentally induced neuropathological defects. An immunocytochemical approach.
The lysosome-associated cathepsins B and D were localized by means of immunocytochemistry (peroxidase-antiperoxidase technique) in glial cells of rat brain. A specific reaction pattern of hippocampal neuroglia could be observed after intracerebroventricular application of the neurotoxin kainic acid. After the induction of a focal epilepsy in rats by the implantation of cobalt pellets there was a pronounced immunoreaction of glia near the primary focus as well as the mirror focus. It is concluded that both cathepsins are useful immunocytochemical markers to trace functionally activated glia.
D000818:Animals; D002401:Cathepsin B / Q000378:metabolism*; D002402:Cathepsin D / Q000378:metabolism*; D003035:Cobalt / Q000633:toxicity; D004828:Epilepsies, Partial / Q000139:chemically induced / Q000201:enzymology; D005260:Female; D006651:Histocytochemistry; D007120:Immunochemistry; D007608:Kainic Acid / Q000633:toxicity; D008297:Male; D009457:Neuroglia / Q000201:enzymology*; D051381:Rats; D011919:Rats, Inbred Strains
D003035:Cobalt; D002401:Cathepsin B; D002402:Cathepsin D; D007608:Kainic Acid