Modulation of Bacillus subtilis alpha-amylase promoter activity by the presence of a palindromic sequence in front of the gene.
Upstream of the promoter of the Bacillus subtilis alpha-amylase gene (amyE) derived from an alpha-amylase hyper-producing strain, there is an inverted repeat sequence (palindromic sequence), which has a free energy of 21.2 kcal/mol due to the formation of stable stem-loop structure. The role of the palindromic sequence for the expression of amyE was studied using a plasmid encoding the amyE'-'bla (E. coli beta-lactamase) fused gene in an alpha-amylase-deficient B. subtilis mutant as the host. By the presence of the palindromic sequence, the transcription activity of the amyE promoter was enhanced approximately 6 fold by starch (3%) in the medium and was less repressed by glucose.
D001412:Bacillus subtilis / Q000201:enzymology* / Q000235:genetics; D001483:Base Sequence; D003470:Culture Media; D005947:Glucose / Q000494:pharmacology; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic; D000516:alpha-Amylases / Q000235:genetics*
D003470:Culture Media; D000516:alpha-Amylases; D005947:Glucose