Determination of aflatoxin M1 in milk and milk products contaminated at low levels.
A new method is described for the determination of aflatoxin M1 in milk and dairy products by thin layer chromatography. The main characteristic is the extraction system using an alkaline solution. Lipids are removed by centrifuging at low temperatures, and the aflatoxins are then extracted with CHCl3. The method has 2 options: Technique II (detection limit 0.02 ppb) requires cleanup on a chromatographic column; this is not necessary in Technique I (detection limit 0.1 ppb). The recovery rate in both techniques is over 92.8% in milk and yoghurt. This method may also be used for other aflatoxins. Because of the advantages of the method, Technique II is recommended for aflatoxin M1 control in milk, where a low detection limit is necessary. Technique I is proposed for experimental aflatoxin production studies in dairy products, which require analysis of a large number of samples but which do not require a very low detection limit.
D016604:Aflatoxin B1; D016607:Aflatoxin M1; D000348:Aflatoxins / Q000032:analysis*; D000818:Animals; D002417:Cattle; D002855:Chromatography, Thin Layer; D003611:Dairy Products / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis*; D005516:Food Microbiology; D007202:Indicators and Reagents; D008892:Milk / Q000032:analysis*; D012997:Solvents
D000348:Aflatoxins; D007202:Indicators and Reagents; D012997:Solvents; D016607:Aflatoxin M1; D016604:Aflatoxin B1