T-independent form of azophenylarsonate fails to reveal "silent" idiotype-positive B cells in idiotype-suppressed mice.
In three distinct chronic Ig-specific suppression systems in which suppression was initiated by injection of mice with anti-idiotype (Id) or anti-allotype sera, evidence has been presented by others that the differentiation of B cells bearing surface Ig with a target marker (Id or allotype) need not be totally disrupted. Normally "silent" Id+ B cells from Id-suppressed mice could be revealed by certain procedures, one of these being to make use of the powerful stimulatory properties of bacterial lipopolysaccharide. We have employed a similar strategy to determine whether cryptic CRIA+ B cells exist in significant numbers in hyperimmune, CRI-suppressed (HIS) A/J mice. Thus, following T cell removal, the Ar-specific B cell repertoire from HIS mice was probed using Ar- Brucella abortus as a T-independent Ag. No evidence for "silent" CRIA+ B cells was found. The results, taken in conjunction with those of others, suggests that there may exist multiple forms of long-term Ig-specific suppression.
D000818:Animals; D000955:Antigens, T-Independent / Q000276:immunology*; D001391:Azo Compounds / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology*; D002003:Brucella abortus / Q000276:immunology; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D007156:Immunologic Memory; D007165:Immunosuppression Therapy; D051379:Mice; D010132:p-Azobenzenearsonate / Q000276:immunology*
D000955:Antigens, T-Independent; D001391:Azo Compounds; D007130:Immunoglobulin Idiotypes; D010132:p-Azobenzenearsonate