Oligoclonal antibodies in CSF of patients with meningopolyneuritis Garin-Bujadoux-Bannwarth: Ig class, light chain type and specificity.
Detection of intrathecally produced antibodies in cerebrospinal fluid (CSF) of patients with meningopolyneuritis Garin-Bujadoux-Bannwarth (MPN-GBB) is well documented. Analysis of CSF has revealed the oligoclonal nature of these antibodies. We investigated oligoclonal antibodies (OA) in CSF and serum of MPN-GBB patients with regard to immunoglobulin class, light chain type and specificity and compared the findings with those in other neurological diseases (OND). For this purpose an immunofixation (IF) technique after agarosegel electrophoresis (AE) of concentrated CSF was used. 87% of patients with MPN-GBB demonstrated in the acute stage of their disease oligoclonal bands (OB) in their CSF which could not be detected in paired serum samples. IF revealed in most cases of MPN-GBB IgG banding. While IgM banding was a common finding in CSF of MPN-GBB patients, this was not the case in OND. Specificity of CSF OA against components of Borrelia burgdorferi could be demonstrated by agarose isoelectric focusing (AIEF), transfer to nitrocellulose paper and reaction with 125I labelled B. burgdorferi antigen.
D000907:Antibodies, Bacterial / Q000134:cerebrospinal fluid*; D000918:Antibody Specificity; D001345:Autoradiography; D001898:Borrelia / Q000276:immunology*; D001899:Borrelia Infections / Q000134:cerebrospinal fluid / Q000276:immunology*; D004587:Electrophoresis, Agar Gel; D006801:Humans; D007147:Immunoglobulin Light Chains / Q000134:cerebrospinal fluid; D007136:Immunoglobulins / Q000134:cerebrospinal fluid; D007158:Immunologic Techniques; D007525:Isoelectric Focusing; D008581:Meningitis / Q000134:cerebrospinal fluid / Q000276:immunology*; D011115:Polyneuropathies / Q000134:cerebrospinal fluid / Q000276:immunology*
D000907:Antibodies, Bacterial; D007147:Immunoglobulin Light Chains; D007136:Immunoglobulins