[A variety of human autoantibodies recognizes in HeLa cells 2 proteins related to the TFIIIA factor of Xenopus laevis which regularizes the transcription of ribosomal 5S RNA].
Using the sera from two patients with autoimmune disorders, we have identified by immunoprecipitation of HeLa cell extracts two proteins with apparent molecular masses of 37 kDa (p 37) and 32 kDa (p 32). These proteins are associated with 5 S RNA. They are antigenetically related to Xenopus laevis 5 S RNA transcription factor TFIIIA, which is very abundant in early oocytes of this species. In contrast to what is observed in X. laevis oocytes, the TFIIIA-related proteins of HeLa cells are present in very small amounts. Our data suggest that proteins p 37 and p 32 are involved in the control of 5 S RNA transcription.
D000818:Animals; D001323:Autoantibodies / Q000276:immunology*; D011232:Chemical Precipitation; D006367:HeLa Cells; D006801:Humans; D008970:Molecular Weight; D035582:Transcription Factor TFIIIA; D014157:Transcription Factors / Q000032:analysis* / Q000276:immunology; D014982:Xenopus laevis
D001323:Autoantibodies; D035582:Transcription Factor TFIIIA; D014157:Transcription Factors
Une variété d'autoanticorps humains reconnaît dans les cellules HeLa deux protéines apparentées au facteur TFIIIA de Xenopus laevis qui régule la transcription du RNA ribosomique 5S.