Appearance of plasminogen activator activity during a synchronous cycle of a rat adenocarcinoma cell line, PA-III.
A study of the appearance of plasminogen activators during the cell cycle of a rat prostate adenocarcinoma cell line, PA-III, was undertaken. After release from a hydroxyurea (HU) block, the length of the cell cycle was determined and found to be 20-25 h, with the S-G2-M portions comprising approx. 10-15 h and the G1 phase occurring over a similar 10-15-h period. Assays for tissue-like plasminogen activator (t-PA) and urokinase-like plasminogen activator (u-PA) in cell-conditioned medium revealed an increased appearance of both enzymes shortly after the S phase of the cycle. The pattern of production of these activators remained the same under differing conditions of cell synchronization.
D000230:Adenocarcinoma / Q000201:enzymology / Q000473:pathology*; D000818:Animals; D002453:Cell Cycle / Q000187:drug effects; D002460:Cell Line; D002999:Clone Cells; D004261:DNA Replication / Q000187:drug effects; D006918:Hydroxyurea / Q000494:pharmacology; D007700:Kinetics; D008297:Male; D011471:Prostatic Neoplasms / Q000201:enzymology / Q000473:pathology*; D051381:Rats; D013936:Thymidine / Q000378:metabolism; D010959:Tissue Plasminogen Activator / Q000096:biosynthesis*; D014568:Urokinase-Type Plasminogen Activator / Q000096:biosynthesis*
D010959:Tissue Plasminogen Activator; D014568:Urokinase-Type Plasminogen Activator; D013936:Thymidine; D006918:Hydroxyurea