Isolation of complementary DNA clones encoding the human lymphocyte glycoprotein T1/Leu-1.
The T1/Leu-1/CD5 molecule, a human T-cell surface glycoprotein of relative molecular mass (Mr) 67,000, has been implicated in the proliferative response of activated T cells and in T-cell helper function. A similar involvement in T-cell proliferation has been reported for Ly-1, the murine homologue of T1. Here we report the complete amino-acid sequence of the T1 precursor molecule deduced from complementary DNA clones. The protein contains a classical signal peptide; a 347-amino-acid extracellular segment; a transmembrane region; and a 93-amino-acid intracellular segment. The extracellular segment contains many cysteine residues and is composed of two related structural domains separated by a proline/threonine-rich region. The T1 molecule has structural features characteristic of other receptor molecules.
D000595:Amino Acid Sequence; D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D003545:Cysteine / Q000032:analysis; D004247:DNA / Q000235:genetics; D006023:Glycoproteins / Q000235:genetics*; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics; D008970:Molecular Weight; D010750:Phosphoproteins / Q000235:genetics; D021382:Protein Sorting Signals / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D011971:Receptors, Immunologic / Q000235:genetics*; D013601:T-Lymphocytes / Q000502:physiology*
D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface; D006023:Glycoproteins; D008565:Membrane Proteins; D010750:Phosphoproteins; D021382:Protein Sorting Signals; D012333:RNA, Messenger; D011971:Receptors, Immunologic; D004247:DNA; D003545:Cysteine