Effects of DL-alpha-difluoromethylornithine on the growth and metastasis of B16 melanoma in vivo.
The effects of DL-alpha-difluoromethylornithine (DFMO), a specific irreversible inhibitor of ornithine decarboxylase, on the growth of experimental mouse B16-F10 melanoma cells were investigated. DFMO (3%) in drinking water was administered to B16-F10 melanoma-bearing mice. At 24 days, B16-F10 melanomas in DFMO-fed mice weighed 75% less than those in control mice (p less than 0.001). DFMO reduced putrescine and spermidine levels in B16-F10 melanoma by 98% and 84%, respectively, and prolonged the mean survival time from 25.9 +/- 1.2 to 35.7 +/- 2.2 days (p less than 0.001). The effects of DFMO on experimental metastasis were also investigated. DFMO treatment resulted in a significant decrease in pulmonary metastasis induced by i.v. injection of B16-F10 melanoma cells.
D000818:Animals; D000518:Eflornithine / Q000494:pharmacology*; D005260:Female; D008175:Lung Neoplasms / Q000556:secondary; D008545:Melanoma / Q000032:analysis / Q000473:pathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D011073:Polyamines / Q000032:analysis
D011073:Polyamines; D000518:Eflornithine