title: Estimation of apparent quadrupolar coupling constants for complexes of magnesium ions with mono- and dicarboxylic acid ligands. Applications to magnesium ion: protein interactions.
abstract: 25Mg+2 ion NMR studies of complexes of magnesium ions with acetate and malonate ligands have yielded apparent quadrupolar coupling constants, chi, of approximately 1.5 MHz. The aquo magnesium ion yields a smaller chi value of 0.12 MHz, consistent with its expected higher symmetry. chi values for magnesium ion: acetate and magnesium ion: malonate complexes are utilized to calculate observed linewidths for magnesium ion: bovine prothrombin fragment 1 and magnesium ion: human Factor XII interactions. These calculated values are compared with observed values and implications of the agreement are discussed.
mesh_terms: D000085:Acetates; D000818:Animals; D002417:Cattle; D005169:Factor VIII / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D008274:Magnesium / Q000378:metabolism; D008314:Malonates; D011485:Protein Binding; D011516:Prothrombin / Q000378:metabolism; D013329:Structure-Activity Relationship
chemical_list: D000085:Acetates; D008314:Malonates; D011516:Prothrombin; D005169:Factor VIII; D008274:Magnesium