Monoclonal antibodies against acetylcholinesterase from electric organs of Electrophorus and Torpedo.
We studied the reactivity of monoclonal antibodies (mAbs) raised against acetylcholinesterase (AChE) purified from Electrophorus and Torpedo electric organs. We obtained IgG antibodies (Elec-21, Elec-106, Tor-3E5, Tor-ME8, Tor-1A5), all of them directed against the catalytic subunit of the corresponding species, with no significant cross-reactivity. These antibodies do not inhibit the enzyme and recognize all molecular forms, globular (G) and asymmetric (A). Tor-ME8 reacts specifically with the denatured A and G subunits of Torpedo AChE, in immunoblots. Several hybridomas raised against Electrophorus AChE produced IgM antibodies (Elec-39, Elec-118, Elec-121). These antibodies react with the A forms of Electrophorus electric organs and also with a subset of dimers (G2) from Torpedo electric organ. In addition, they react with a number of non-AChE components, in immunoblots. In contrast, they do not recognize AChE from other Electrophorus tissues or A forms from Torpedo electric organs.
D000110:Acetylcholinesterase / Q000032:analysis* / Q000276:immunology; D000818:Animals; D000911:Antibodies, Monoclonal; D002091:Butyrylcholinesterase / Q000276:immunology; D002499:Centrifugation, Density Gradient; D003101:Collodion; D003429:Cross Reactions; D004557:Electric Organ / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel; D004593:Electrophorus; D007124:Immunoenzyme Techniques; D011863:Radioimmunoassay; D014101:Torpedo
D000911:Antibodies, Monoclonal; D003101:Collodion; D000110:Acetylcholinesterase; D002091:Butyrylcholinesterase