title: [Studies on the diagnosis of schistosomiasis japonica. (4). Effects of serum dialysis and anthelmintic treatment of the patients on the determination of COP value with special reference to the black eggs].
abstract: Eggs of Schistosoma japonicum were obtained from the livers and intestines of the mice infected subcutaneously 50 cercaria 7 weeks previously. A dialyzing celophane tube containing 1 ml of serum was immersed and stirred in 500 ml NaCl solution with different molar concentrations and dialyzed for 24 hrs at 4C. Thereafter, the sera of infected human and animals were incubated with an equal volume of egg-suspension (average 100 eggs per drop). The results indicated that a close relationship between a decrease in the molar concentration of dialyzing solution and an increase of COP value. COP value in a given dialyzed serum sample was dependent on the molar concentration of the solution and independent of the different salt solution with KCl, CaCl2 and MgCl2. COP values were observed in 44 patients in 12 months after treatment with niridazole. The results were as follows; unchanged 1 case, turned to negative 1 case, moderately decreased 3 cases and marked decreased 39 cases. Re-dialysis of these sera with 0.15 M NaCl solution resulted in the equal COP value in all cases. Blackeggs showed a marked reactivity against antisera in COP test as well as those of normal matured eggs.
mesh_terms: D000818:Animals; D003956:Dialysis; D005260:Female; D006801:Humans; D008297:Male; D009560:Niridazole / Q000627:therapeutic use; D010063:Ovum / Q000276:immunology; D011233:Precipitin Tests; D012549:Schistosoma japonicum / Q000276:immunology; D012554:Schistosomiasis japonica / Q000175:diagnosis* / Q000188:drug therapy
chemical_list: D009560:Niridazole