Prostaglandin synthesis and release from cultured human trabecular-meshwork cells and scleral fibroblasts.
Human trabecular-meshwork (HTM) cells in culture convert arachidonic acid to two products: PGE2 and 6-keto PGF1 alpha. Prostaglandin PGE2 was the major product of arachidonic-acid metabolism. The synthesis and release of PGE2 and 6-keto PGF1 alpha was inhibited by a 15-min pre-treatment with indomethacin (5 X 10(-6) M) or a 4-24-hr incubation with 10(-7) M dexamethasone. The effects of dexamethasone could be prevented by cycloheximide (0.5 micrograms ml-1), or actinomycin D (2 micrograms ml-1). Prostaglandin E2 synthesis and release by these HTM cells from two different individuals could be stimulated by bradykinin, arachidonic acid and the calcium ionophore, A23187.
D015121:6-Ketoprostaglandin F1 alpha / Q000096:biosynthesis*; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism; D001920:Bradykinin / Q000494:pharmacology; D000001:Calcimycin / Q000494:pharmacology; D002478:Cells, Cultured; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D003907:Dexamethasone / Q000037:antagonists & inhibitors; D015232:Dinoprostone; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology; D011450:Prostaglandin Endoperoxides, Synthetic / Q000494:pharmacology; D044262:Prostaglandin H2; D011458:Prostaglandins E / Q000096:biosynthesis*; D011463:Prostaglandins H / Q000494:pharmacology; D012590:Sclera / Q000378:metabolism*; D014129:Trabecular Meshwork / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*
D001095:Arachidonic Acids; D011450:Prostaglandin Endoperoxides, Synthetic; D011458:Prostaglandins E; D011463:Prostaglandins H; D003609:Dactinomycin; D016718:Arachidonic Acid; D000001:Calcimycin; D044262:Prostaglandin H2; D015121:6-Ketoprostaglandin F1 alpha; D003907:Dexamethasone; D003513:Cycloheximide; D015232:Dinoprostone; D001920:Bradykinin; D007213:Indomethacin