[Construction of plasmids integrating into the Bacillus subtilis chromosome through homologous recombination and their use as integration vectors].
We constructed a number of plasmids which integrate into the chromosome of Bacillus subtilis through homology recombination. Plasmids consist of pBR322 replicon, different fragments of Bac. subtilis chromosomal DNA, Cm resistance marker from pBD64 plasmid. Frequency of transformation was 10(-4) per bacterial cell. Foreign DNA (genes for tryptophan metabolism of Bac. mesentericus) was introduced into the chromosome of Bac. subtilis with the help of these plasmids.
D001412:Bacillus subtilis / Q000235:genetics* / Q000378:metabolism; D002876:Chromosomes, Bacterial; D004269:DNA, Bacterial / Q000235:genetics; D005819:Genetic Markers; D005822:Genetic Vectors; D009876:Operon; D010957:Plasmids; D012689:Sequence Homology, Nucleic Acid; D014169:Transformation, Bacterial; D014364:Tryptophan / Q000378:metabolism
D004269:DNA, Bacterial; D005819:Genetic Markers; D014364:Tryptophan
Konstruirovanie plazmid, vstraivaiushchikhsia v khromosomu Bacillus subtilis za schet gomologichnoĭ rekombinatsii, i ispol'zovanie ikh kak integrativnykh vektorov.