Assessment of binding of L-cystine and L-lysine by rat renal brush-border membranes.
Cystine and lysine bind to isolated rat renal brush-border vesicles. Three methods to determine the extent of amino acid binding to the membranes have been compared, one relying on the osmotic reactivity of the vesicle, a second by trichloroacetic acid precipitation of membrane-bound material and a third by initial rate analysis. For cystine, all methods yield comparable results at early time points, indicating the trichloroacetic acid method is a simple and valuable tool for binding estimation under initial-rate or near initial-rate conditions. For lysine, initial rate analysis and osmotic perturbation are the methods of choice since lysine co-precipitates with trichloroacetic acid.
D000818:Animals; D011232:Chemical Precipitation; D003553:Cystine / Q000378:metabolism*; D007668:Kidney / Q000378:metabolism*; D007700:Kinetics; D008239:Lysine / Q000378:metabolism*; D008871:Microvilli / Q000378:metabolism*; D009994:Osmolar Concentration; D051381:Rats; D014238:Trichloroacetic Acid
D003553:Cystine; D014238:Trichloroacetic Acid; D008239:Lysine