title: Simultaneous determination of carbohydrates and products of carbohydrate metabolism in fermentation mixtures by HPLC.
abstract: An improved procedure for separating and quantitating carbohydrates, alcohols, and organic acids in fermentation mixtures metabolized by intestinal microflora is described. The high-pressure liquid chromatographic method is efficient, reproducible, and sensitive. A column packed with cation-exchange resin in the hydrogen form, eluted isocratically with 0.028 M H2SO4 at 40 degrees C separates the compounds of interest. The eluate is monitored with ultraviolet and refractive index detectors in series. On-line acquistion and storage of detector output by a computer allows post-analysis data manipulation and quantitation. Using this method, the metabolic profiles for the fermentation of glucose, fructose, lactose, and sucrose by several intestinal microorganisms are characterized and compared.
mesh_terms: D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D013293:Enterococcus faecalis / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism*; D005285:Fermentation; D007711:Klebsiella pneumoniae / Q000378:metabolism*
chemical_list: D002241:Carbohydrates