title: Organization of genes for ribosomal proteins S7 and S12, elongation factors EF-Tu and EF-G in the cyanobacterium Spirulina platensis.
abstract: The gene encoding ribosomal proteins S12 and probably S7 as well as protein synthesis elongation factors Tu (EF-Tu) and G (EF-G) of Spirulina platensis have been identified and cloned. Gene expression was determined for ribosomal protein S12 by genetic complementation of the appropriate Escherichia coli mutant, whereas for the EF-Tu gene it was determined by production of the protein in E. coli minicells. On the basis of these experiments we suggest the following gene order in the S. platensis chromosome: S12, S7, EF-G, EF-Tu.
mesh_terms: D003001:Cloning, Molecular; D000458:Cyanobacteria / Q000502:physiology*; D004926:Escherichia coli / Q000502:physiology; D005786:Gene Expression Regulation; D005796:Genes; D005821:Genetic Techniques; D020653:Peptide Elongation Factor G; D010444:Peptide Elongation Factor Tu / Q000235:genetics*; D010445:Peptide Elongation Factors / Q000235:genetics*; D012269:Ribosomal Proteins / Q000235:genetics*
chemical_list: D020653:Peptide Elongation Factor G; D010445:Peptide Elongation Factors; D012269:Ribosomal Proteins; D010444:Peptide Elongation Factor Tu