Techniques for locating isotopically labelled schistosomula of Schistosoma mansoni in host tissues for ultrastructural investigations.
The use of 75 Selenomethionine labelled cercariae of Schistosoma mansoni for ultrastructural localization of parasites in host tissues has been evaluated. Both gamma counting of tissue, and autoradiography of resin-embedded tissue were successful. The autoradiographic technique was more sensitive and schistosomula were readily located in pulmonary tissue up to 24 days post-infection.
D000818:Animals; D001345:Autoradiography; D007668:Kidney / Q000469:parasitology*; D008168:Lung / Q000469:parasitology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008854:Microscopy, Electron; D012550:Schistosoma mansoni / Q000648:ultrastructure*; D012555:Schistosomiasis mansoni / Q000469:parasitology*; D012588:Scintillation Counting; D012645:Selenomethionine
D012645:Selenomethionine