Identification of 5-S-cysteinyldopa by high performance liquid chromatography in biopsies from patients with dysplastic melanocytic nevi.
The appearance of 5-[(L)-S-cysteinyl]dopa, a major product in pheomelanogenesis was examined in affected and nonaffected skins from 20 patients with clinical signs of dysplastic melanocytic nevi. Analysis by high performance liquid chromatography and electrochemical detection showed that 20 of the 35 lesions had a pathological formation of 5-[(L)-S-cysteinyl]dopa (0.04-28.86 ng/micrograms acid soluble protein). 5-[(L)-S-cysteinyl]dopa was not detected in any of the normal uninvolved skin samples analyzed.
D001706:Biopsy; D002851:Chromatography, High Pressure Liquid; D003548:Cysteinyldopa / Q000032:analysis*; D004295:Dihydroxyphenylalanine / Q000031:analogs & derivatives*; D006801:Humans; D008544:Melanocytes / Q000032:analysis; D009508:Nevus, Pigmented / Q000032:analysis*; D011230:Precancerous Conditions / Q000032:analysis; D012867:Skin / Q000032:analysis
D003548:Cysteinyldopa; D004295:Dihydroxyphenylalanine