title: Orthogonal arrays of particles in the gastric parietal cell of the rat: differences between superficial and basal cells in the gland and after pentagastrin or metiamide treatment.
abstract: The basolateral plasma membrane of gastric parietal cells is characterized by the presence of orthogonal arrays of particles revealed by the freeze-fracture technique. These arrays were quantitatively assessed in freeze-fracture replicas of the gastric mucosa at two different levels of the gastric gland and after pentagastrin and metiamide treatment. The arrays were small and scarce in parietal cells located in the upper part of the gland, while they were markedly more abundant in parietal cells situated at the base of the gland. In both superficial and basal cells, the concentration of the arrays was significantly decreased after pentagastrin or metiamide treatment. This decrease was not due to an increase in the surface area of the basal plasma membrane. These results indicate that the concentration of the orthogonal arrays is a distinctive feature between superficial and basal parietal cells and that the arrays can be modulated by parietal cell function.
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000187:drug effects / Q000648:ultrastructure; D005260:Female; D005614:Freeze Fracturing; D005753:Gastric Mucosa / Q000187:drug effects / Q000648:ultrastructure; D007274:Injections, Intraperitoneal; D008433:Mathematics; D008785:Metiamide / Q000008:administration & dosage / Q000494:pharmacology*; D008854:Microscopy, Electron; D010295:Parietal Cells, Gastric / Q000187:drug effects / Q000648:ultrastructure*; D010418:Pentagastrin / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D013890:Thiourea / Q000031:analogs & derivatives*; D013997:Time Factors
chemical_list: D008785:Metiamide; D010418:Pentagastrin; D013890:Thiourea