Urine recovery experiments with quercetin and other mutagens using the Ames test.
Recovery from urine of the mutagenic activity of 2-anthramine, cyclophosphamide, 7,12-dimethylbenz[a]anthracene, 6-chloro-9-((3-(2-chloroethylamino)-propyl)amino)-2-methoxyacridin e dihydrochloride (ICR-191), mitomycin-C, nitrofurantoin, and quercetin was studied with several of the Ames tester strains using acetone-extracted XAD-2 columns with yields ranging from 27% to 79%. Dose responses of the pure chemicals were also studied, and results showed TA 97 to be far more susceptible to quercetin mutagenesis than TA 1537. Reducing pour plate agar volume enhanced mutagenesis.
D015127:9,10-Dimethyl-1,2-benzanthracene / Q000494:pharmacology / Q000652:urine; D000585:Aminacrine / Q000031:analogs & derivatives / Q000494:pharmacology / Q000652:urine; D000818:Animals; D000873:Anthracenes / Q000494:pharmacology / Q000652:urine; D002852:Chromatography, Ion Exchange; D003520:Cyclophosphamide / Q000494:pharmacology / Q000652:urine; D004305:Dose-Response Relationship, Drug; D005419:Flavonoids / Q000494:pharmacology*; D006801:Humans; D016685:Mitomycin; D008937:Mitomycins / Q000494:pharmacology / Q000652:urine; D009152:Mutagenicity Tests; D009153:Mutagens / Q000378:metabolism / Q000494:pharmacology*; D009582:Nitrofurantoin / Q000494:pharmacology / Q000652:urine; D009588:Nitrogen Mustard Compounds / Q000494:pharmacology / Q000652:urine; D011137:Polystyrenes; D011794:Quercetin / Q000494:pharmacology* / Q000652:urine; D051381:Rats; D012486:Salmonella typhimurium / Q000187:drug effects
D000873:Anthracenes; D005419:Flavonoids; D008937:Mitomycins; D009153:Mutagens; D009588:Nitrogen Mustard Compounds; D011137:Polystyrenes; D016685:Mitomycin; D015127:9,10-Dimethyl-1,2-benzanthracene; D000585:Aminacrine; C006593:2-anthramine; D003520:Cyclophosphamide; C003771:Amberlite XAD-2 resin; D009582:Nitrofurantoin; C004294:acridine half-mustard; D011794:Quercetin