Requirement of monohydroperoxy fatty acids for the oxygenation of 15LS-HETE by reticulocyte lipoxygenase.
The lipoxygenase from reticulocytes oxygenates 15LS-HETE to 8-hydroperoxy-15-hydroxy-5,9,11,13-eicosatetraenoic acid and 5-hydroperoxy-15-hydroxy-6,8,11,13-eicosatetraenoic acid only in the presence of catalytic concentrations of monohydroperoxy fatty acids. During this reaction the hydroperoxy fatty acids are converted to more polar products including hydroxy fatty acids. From kinetic measurements of 15LS-HETE oxygenation it was calculated that 1 mol monohydroperoxy fatty acid is consumed during the oxygenation of about 9 mol 15LS-HETE.
D005227:Fatty Acids / Q000378:metabolism / Q000494:pharmacology*; D006893:Hydroxyeicosatetraenoic Acids / Q000378:metabolism*; D066298:In Vitro Techniques; D007700:Kinetics; D008041:Linoleic Acids / Q000378:metabolism / Q000494:pharmacology; D008084:Lipoxygenase / Q000494:pharmacology*; D013058:Mass Spectrometry; D010100:Oxygen / Q000378:metabolism; D012156:Reticulocytes / Q000201:enzymology; D013696:Temperature
D005227:Fatty Acids; D006893:Hydroxyeicosatetraenoic Acids; D008041:Linoleic Acids; C025984:15-hydroxy-5,8,11,13-eicosatetraenoic acid; D008084:Lipoxygenase; C032890:13-hydroperoxylinoleic acid; D010100:Oxygen