Cell surface phenotype of the spontaneous immunoglobulin-secreting cells in peripheral blood from homosexual men with generalized lymphadenopathy or AIDS.
Activated B cells that spontaneously secrete immunoglobulin are found in homosexual men with AIDS or lymphadenopathy. These cells constitute a very small percentage of peripheral blood lymphocytes (usually less than 1%), making identification of their surface antigens difficult. To identify surface antigens on immunoglobulin-secreting cells, peripheral blood mononuclear cells were first reacted with monoclonal antibody, followed by a fluorescein-conjugated goat antimouse globulin reagent. Secretion of immunoglobulin was then assessed in a reverse hemolytic plaque assay, with a modified Cunningham chamber in which an individual plaque-forming cell could be examined with a fluorescence microscope. All plaque-forming cells were found to be reactive with OKT 10 and 4F2 monoclonal antibodies; there was moderate reactivity with anti-la and B4. The same results were found when normal pokeweed mitogen-stimulated lymphocytes were tested. The surface phenotype of these cells is consistent with that of a preplasma cell.
D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology*; D000328:Adult; D000921:Antibody-Producing Cells / Q000276:immunology*; D000944:Antigens, Differentiation, B-Lymphocyte; D000954:Antigens, Surface / Q000032:analysis; D002454:Cell Differentiation; D005260:Female; D006462:Hemolytic Plaque Technique; D006716:Homosexuality; D006801:Humans; D008206:Lymphatic Diseases / Q000276:immunology*; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D010950:Plasma Cells / Q000276:immunology; D011947:Receptors, Antigen, B-Cell / Q000032:analysis
D000944:Antigens, Differentiation, B-Lymphocyte; D000954:Antigens, Surface; D011947:Receptors, Antigen, B-Cell