Distinct culture requirements for activation and proliferation of human peripheral blood B lymphocytes by Staphylococcus aureus Cowan strain and growth factors.
The concentration of fetal calf serum (FCS) and the culture period were the crucial culture conditions in measuring B cell growth factor (BCGF) and IL-2 activity in vitro. Higher concentrations of FCS (10-15%) significantly inhibited BCGF activity; whereas, lower concentrations of FCS (less than 2.5%) were not sufficient for the response to IL-2. Kinetic experiments showed that the culture period for BCGF should be shorter than that for IL-2, while BCGF in combination with IL-2 induced a synergistic proliferation of B cells in a longer culture period. Adding BCGF after 48 h of SAC stimulation reduced the reaction. Hence, the conventional method using preactivated B cells does not measure BCGF but mostly IL-2. Furthermore, minute amounts of BCGF activity can be more sensitively determined by co-culturing with fixed amounts of IL-2.
D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D002417:Cattle; D002478:Cells, Cultured; D003470:Culture Media; D004357:Drug Synergism; D005312:Fetal Blood / Q000502:physiology; D006133:Growth Substances / Q000032:analysis / Q000502:physiology*; D006801:Humans; D007371:Interferon-gamma / Q000502:physiology; D007376:Interleukin-2 / Q000502:physiology; D015847:Interleukin-4; D007700:Kinetics; D008213:Lymphocyte Activation / Q000187:drug effects; D008222:Lymphokines / Q000032:analysis / Q000502:physiology*; D013211:Staphylococcus aureus / Q000145:classification / Q000502:physiology*; D013997:Time Factors
D003470:Culture Media; D006133:Growth Substances; D007376:Interleukin-2; D008222:Lymphokines; D015847:Interleukin-4; D007371:Interferon-gamma