title: Forward motility protein (FMP): localization in hamster epididymal spermatozoa.
abstract: The presence of FMP was investigated by immunocytochemistry in spermatozoa from the hamster caput and cauda epididymis. Spermatozoa from the caput showed no staining whereas spermatozoa pre-incubated with FMP were stained on the acrosome. Pre-treatment of the same sperm with epididymal plasma induced staining on the principal piece of the flagellum. Spermatozoa from the cauda were stained without previous incubation both on the acrosome and on the principal piece of the flagellum. These results suggest that the action of FMP, which prevents head-to-head agglutination of motile spermatozoa and allows acquisition of forward motility, may be due to at least two proteins. The first localizes to the acrosome during epididymal transit (anti-sticking factor), whilst the second localizes to the principal piece of the flagellum (forward motility initiation factor).
mesh_terms: D000177:Acrosome / Q000032:analysis; D000818:Animals; D011232:Chemical Precipitation; D006224:Cricetinae; D004822:Epididymis; D006651:Histocytochemistry; D005779:Immunodiffusion; D007158:Immunologic Techniques; D008297:Male; D008970:Molecular Weight; D011506:Proteins / Q000032:analysis*; D013081:Sperm Motility; D013094:Spermatozoa / Q000032:analysis*
chemical_list: D011506:Proteins; C029700:sperm forward motility protein, Bos taurus