Measurement of semen quality, fertility, and reproductive hormones to assess dibromochloropropane (DBCP) effects in live rabbits.
Thirty-six sexually mature Dutch rabbits were divided into six equal groups to receive in the drinking water 5 days/week for 10 weeks 0, 0.94, 1.88, 3.75, 7.50, and 15.00 mg of DBCP/kg body wt. General health, body weight, semen quality (four ejaculates per male per week), and libido were measured throughout. Fertility, blood follicle stimulating hormone (FSH), luteinizing hormone (LH), and testosterone were measured the last week and cauda epididymal sperm were examined at sacrifice. There was no effect of DBCP on general health or body weight. There was considerable variation in ejaculate volume, percentage motile sperm, and sperm concentration per milliliter within groups and among weeks. However, between the first 2 weeks and the last 2 weeks of the experiment sperm output had increased by 19% in the three lower DBCP groups and decreased by 16% in the three higher DBCP groups (p less than 0.01). The proportion of sperm with abnormal tails also increased as DBCP dosage increased. Fertility was unaffected. FSH was elevated (p less than 0.01) in the group receiving 15 mg/kg of DBCP, which is consistent with the impairment of spermatogenesis. Libido, LH, and testosterone levels were not affected. Sperm morphology was the most sensitive indicator of a DBCP effect in the live animal, being affected at a daily oral intake greater than or equal to 1.88 mg DBCP/kg body wt.
D000818:Animals; D004822:Epididymis / Q000187:drug effects / Q000473:pathology; D005298:Fertility / Q000187:drug effects*; D005640:Follicle Stimulating Hormone / Q000097:blood; D012739:Gonadal Steroid Hormones / Q000097:blood*; D007986:Luteinizing Hormone / Q000097:blood; D008297:Male; D011407:Propane / Q000031:analogs & derivatives* / Q000633:toxicity; D011817:Rabbits; D012661:Semen / Q000187:drug effects*; D013091:Spermatogenesis / Q000187:drug effects; D013094:Spermatozoa / Q000002:abnormalities / Q000187:drug effects; D013737:Testis / Q000187:drug effects / Q000473:pathology; D013739:Testosterone / Q000097:blood*
D012739:Gonadal Steroid Hormones; D013739:Testosterone; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; C007318:1,2-dibromo-3-chloropropane; D011407:Propane