title: Glucoamylase of Aspergillus niger.
abstract: Aspergillus niger produces two extracellular glucoamylases (GAI of Mr 85 300 and GAII of Mr 77 600) separable on DEAE-cellulose. The enzymes differes in electrophoretic mobility, thermostability and substrate specificity. The GAI/GAII ratio depends on the concentration and form of nitrogen (nitrate or ammonium) in the culture medium. Proteinase VIII from Bacillus subtilis converts GAI to a form showing properties similar to those of GAII. Possible proteolytic degradation of GAI to GAII by Asp. niger endogenous proteinase(s) is suggested.
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology* / Q000254:growth & development; D003470:Culture Media; D005087:Glucan 1,4-alpha-Glucosidase / Q000302:isolation & purification / Q000378:metabolism*; D005959:Glucosidases / Q000378:metabolism*; D007700:Kinetics
chemical_list: D003470:Culture Media; D005959:Glucosidases; D005087:Glucan 1,4-alpha-Glucosidase