Induction of cyclo-oxygenase by interleukin-1 in rheumatoid synovial cells.
The ability of interleukin-1 (IL-1) to stimulate prostaglandin E2 (PGE2) production by human rheumatoid adherent synovial cells was found to be time-dependent and sensitive to protein synthesis inhibitors. Cells incubated with exogenous arachidonic acid (10 microM) showed no increase in PGE2 production. However, with IL-1 (2.5 U/ml) and exogenous arachidonic acid there was a marked increase, with levels reaching twice that for cells incubated with IL-1 alone. Aspirin pre-treatment studies and the use of [acetyl-14C]aspirin showed that IL-1 increased PGE2 production through the induction of cyclo-oxygenase.
D001095:Arachidonic Acids / Q000494:pharmacology; D001172:Arthritis, Rheumatoid / Q000201:enzymology*; D001241:Aspirin / Q000494:pharmacology; D002460:Cell Line; D015232:Dinoprostone; D004790:Enzyme Induction; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology*; D011451:Prostaglandin-Endoperoxide Synthases / Q000096:biosynthesis*; D011458:Prostaglandins E / Q000096:biosynthesis*; D013583:Synovial Membrane / Q000201:enzymology*
D001095:Arachidonic Acids; D007375:Interleukin-1; D011458:Prostaglandins E; D011451:Prostaglandin-Endoperoxide Synthases; D015232:Dinoprostone; D001241:Aspirin