Structural plasmid instability in recombination- and repair-deficient strains of Bacillus subtilis.
Plasmid pGP1, containing a fusion between the penicillinase gene of Bacillus licheniformis and the beta-galactosidase gene of Escherichia coli, was constructed. This plasmid enabled a study of structural plasmid instability in Bacillus subtilis wild-type cells and a variety of B. subtilis strains, defective in recombination- and DNA-repair functions. Large differences with respect to the level of stability of this plasmid were observed in the various genetic backgrounds.
D001412:Bacillus subtilis / Q000235:genetics*; D004260:DNA Repair; D004274:DNA, Recombinant; D010405:Penicillinase / Q000235:genetics; D010957:Plasmids; D011993:Recombinant Fusion Proteins / Q000235:genetics; D011995:Recombination, Genetic; D001616:beta-Galactosidase / Q000235:genetics
D004274:DNA, Recombinant; D011993:Recombinant Fusion Proteins; D001616:beta-Galactosidase; D010405:Penicillinase