Amplification of human immuno-deficiency virus production from a virus-producing cell line in culture at high cell density.
The amount of infectious virus released into the culture fluid from human immunodeficiency virus (HIV)-producing MOLT-4 cells (MOLT-4/HIV HTLV-III) was measured as a function of the culture conditions and cell density. After 3 days, the daily yield of infectious virus per cell in the fluid of non-growing cells cultured at high cell density was about 15 times higher than the accumulated yield from growing cells cultured at low cell density. SDS-PAGE analysis also showed a similar difference in the amounts of major gag protein p24.
D002460:Cell Line; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel; D006678:HIV / Q000502:physiology*; D006801:Humans; D008565:Membrane Proteins / Q000032:analysis; D013936:Thymidine / Q000031:analogs & derivatives / Q000494:pharmacology; D014764:Viral Proteins / Q000032:analysis; D014771:Virion / Q000032:analysis; D014773:Virology / Q000379:methods; D014779:Virus Replication / Q000187:drug effects; D015215:Zidovudine
D003470:Culture Media; D008565:Membrane Proteins; C046375:NRSN1 protein, human; D014764:Viral Proteins; D015215:Zidovudine; D013936:Thymidine