Oxygen limitation on L-serine production in a hollow-fiber bioreactor.
Pseudomonas AM1 utilizes glycine and methanol to produce L-serine aerobically. The consumption of methanol in this bioconversion is stoichiometrically in excess of L-serine production. Consequently, the oxygen requirement associated with L-serine production is higher than expected for the conversion from glycine. One method of L-serine production investigated was a technique utilizing a hollow-fiber ultrafiltration cartridge as a bioreactor. Oxygen diffusion limitations appear to impede the consumption of methanol and, consequently, the production of L-serine in such a reactor. Methanol consumption data agree with predictions based on a hollow-fiber diffusion model.
D005998:Glycine / Q000378:metabolism; D007700:Kinetics; D000432:Methanol / Q000378:metabolism; D008722:Methods; D010101:Oxygen Consumption; D011549:Pseudomonas / Q000378:metabolism*; D012694:Serine / Q000096:biosynthesis*; D014462:Ultrafiltration
D012694:Serine; D005998:Glycine; D000432:Methanol