Role of protein kinase C in luteinizing hormone-releasing hormone (LHRH)-stimulated progesterone production in rat granulosa cells.
Treatment of rat granulosa cells with LHRH or the phorbol ester TPA stimulated progesterone (P) production during a 5-h incubation. The concomitant presence of a potent inhibitor of protein kinase C attenuated the LHRH effect by ca. 60% and completely blocked the stimulatory effect of TPA. Addition of TPA (10(-9), 10(-8) or 10(-7) M) or dioctanylglycerol (100 micrograms/ml) increased P production; these stimulatory effects were not potentiated by the concomitant presence of a calcium ionophore (A23187, 10(-7) or 10(-6)M). These data support the hypothesis that protein kinase C activation have a role in the steroidogenic action of LHRH in the ovary.
D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000494:pharmacology*; D006107:Granulosa Cells / Q000187:drug effects / Q000378:metabolism*; D066298:In Vitro Techniques; D007546:Isoquinolines / Q000494:pharmacology; D007700:Kinetics; D010703:Phorbol Esters / Q000494:pharmacology; D010879:Piperazines / Q000494:pharmacology; D011374:Progesterone / Q000096:biosynthesis*; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D007546:Isoquinolines; D010703:Phorbol Esters; D010879:Piperazines; D007987:Gonadotropin-Releasing Hormone; D000001:Calcimycin; D011374:Progesterone; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate