Delimiting regulatory sequences of the Drosophila melanogaster Ddc gene.
We delimited sequences necessary for in vivo expression of the Drosophila melanogaster dopa decarboxylase gene Ddc. The expression of in vitro-altered genes was assayed following germ line integration via P-element vectors. Sequences between -209 and -24 were necessary for normally regulated expression, although genes lacking these sequences could be expressed at 10 to 50% of wild-type levels at specific developmental times. These genes showed components of normal developmental expression, which suggests that they retain some regulatory elements. All Ddc genes lacking the normal immediate 5'-flanking sequences were grossly deficient in larval central nervous system expression. Thus, this upstream region must contain at least one element necessary for this expression. A mutated Ddc gene without a normal TATA boxlike sequence used the normal RNA start points, indicating that this sequences is not required for start point specificity.
D000426:Alcohol Dehydrogenase / Q000235:genetics; D000818:Animals; D001142:Aromatic-L-Amino-Acid Decarboxylases / Q000235:genetics*; D001483:Base Sequence; D002872:Chromosome Deletion; D004296:Dopa Decarboxylase / Q000235:genetics*; D004331:Drosophila melanogaster / Q000201:enzymology / Q000235:genetics*; D004351:Drug Resistance; D000431:Ethanol / Q000494:pharmacology; D005796:Genes; D005809:Genes, Regulator; D005822:Genetic Vectors
D000431:Ethanol; D000426:Alcohol Dehydrogenase; D004296:Dopa Decarboxylase; D001142:Aromatic-L-Amino-Acid Decarboxylases