title: Cutaneous tissue repair following CO2 laser irradiation.
abstract: We studied the mechanism of repair following exposure of normal skin to the CO2 laser in a focused mode. Exposed areas were biopsied at 0, 24, 48 h; 1, 2 weeks; 1, 2 1/2 months (pulse width varying from 0.1 to 1.0 s) after irradiation. The initial pattern was a V-shaped zone of cauterized collagen with a central crevice, the depth of which correlated with the total energy applied. The epidermal changes consisted of transepidermal cauterization and basal vacuolar changes lateral to the site of impact. Over a period of 1 week, the wound crevice decreased in depth and width and the central margins of the zone of cauterized collagen approximated. The cauterized collagen was extruded and was noted in the epidermal crust; minimal granulation tissue was present. Biopsies at later time periods showed formation of granulation tissue and retention of small amounts of necrotic collagen; the process of collagen extrusion was largely prevented by suturing. These observations show that dermal contraction and necrotic collagen extrusion are important components of initial tissue repair following limited dermal destruction produced by CO2 irradiation.
mesh_terms: D002245:Carbon Dioxide; D003094:Collagen / Q000378:metabolism; D006801:Humans; D007834:Lasers; D012867:Skin / Q000293:injuries* / Q000378:metabolism / Q000473:pathology; D013537:Sutures; D014945:Wound Healing
chemical_list: D002245:Carbon Dioxide; D003094:Collagen