Corticosteroid-resistant bone marrow-derived B lymphocyte progenitor for long term in vitro cultures.
A long-term in vitro culture system derived from murine bone marrow cells can successfully support the growth of B cell precursors, pre-B cells, and IgM-expressing B cells. Intermediates in the B cell developmental pathway are known to have differential sensitivities to the toxic effects of corticosteroids. We demonstrate here that long term B lineage cultures can be established with the corticosteroid-resistant cell population from bone marrow. Kinetics for the establishment and growth of cultures derived from corticosteroid-treated marrow are similar to those observed with control cultures. Cells obtained from both sets of cultures have similar morphologies and ranges of phenotypic markers. These results indicate that the cell responsible for the outgrowth of the long term B lineage cultures is corticosteroid resistant and is likely to be earlier in the B lymphocyte lineage than steroid-sensitive pre-B cells.
D000011:Abelson murine leukemia virus; D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology*; D001853:Bone Marrow / Q000187:drug effects; D001854:Bone Marrow Cells; D002454:Cell Differentiation; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D004351:Drug Resistance; D006412:Hematopoietic Stem Cells / Q000187:drug effects; D006854:Hydrocortisone / Q000031:analogs & derivatives* / Q000494:pharmacology; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D007145:Immunoglobulin kappa-Chains / Q000235:genetics; D007148:Immunoglobulin mu-Chains / Q000032:analysis; D051379:Mice; D008807:Mice, Inbred BALB C; D011947:Receptors, Antigen, B-Cell / Q000032:analysis; D011995:Recombination, Genetic
D007143:Immunoglobulin Heavy Chains; D007145:Immunoglobulin kappa-Chains; D007148:Immunoglobulin mu-Chains; D011947:Receptors, Antigen, B-Cell; C021650:hydrocortisone acetate; D006854:Hydrocortisone