Analysis of the D-region products of H-2q using monoclonal antibodies reveals the expression of a new class I-like molecule.
To analyze how many D-region-encoded molecules could be detected in H-2q, we produced a panel of nine monoclonal antibodies from AKR (KkDk) anti-AKR.M (KkDq) immunizations. All of the Dq region antibodies cross-reacted on Dd and/or Ld, and all except one cross-reacted on Db, confirming the previously observed serologic and amino acid sequence homology between the D-region products of H-2d, H-2b, and H-2q. All of these monoclonal antibodies precipitated 46,000 dalton molecules from both cell-surface-labeled and biosynthetically labeled B10.AKM spleen cells, indicating that all were reactive with class I-like molecules. Sequential immunoprecipitation analysis with one of these antibodies, 66-3-5, reveals the presence of a previously unidentified class I-like molecule. Tryptic peptide map analysis reveals that this molecule may be the product of a newly described H-2Dq-region gene.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D003429:Cross Reactions; D005802:Genes, MHC Class II; D006183:H-2 Antigens / Q000235:genetics* / Q000276:immunology; D064532:Histocompatibility Antigen H-2D; D051379:Mice; D008806:Mice, Inbred AKR / Q000235:genetics / Q000276:immunology; D008815:Mice, Inbred Strains / Q000235:genetics / Q000276:immunology; D010449:Peptide Mapping; D012689:Sequence Homology, Nucleic Acid
D000911:Antibodies, Monoclonal; D006183:H-2 Antigens; D064532:Histocompatibility Antigen H-2D