Immunohistological analysis of Rosai-Dorfman histiocytosis. A disease of S-100 + CD1-histiocytes.
Five cases of Rosai-Dorfman histiocytosis (RDH) (also called Sinus Histiocytosis with Massive Lymphoadenopathy; SHML) have been studied by immunohistochemical methods with heteroantisera and monoclonal antibodies. One case was also studied by Southern blot hybridization analysis with DNA probes specific for T cell receptor beta chain and immunoglobulin heavy chain. Immunophenotyping of large histiocytes, characteristic of RDH, evidenced the presence of S-100 protein and the absence of CD1 and other markers usually found in histiocytes and macrophages. DNA hybridization study showed the absence of clonal T or B lymphoid populations.
D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface / Q000032:analysis; D004247:DNA / Q000032:analysis; D006644:Histiocytes / Q000145:classification / Q000473:pathology; D006651:Histocytochemistry; D006801:Humans; D007120:Immunochemistry; D008198:Lymph Nodes / Q000473:pathology*; D008206:Lymphatic Diseases / Q000378:metabolism / Q000473:pathology*; D009418:S100 Proteins / Q000032:analysis
D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface; D009418:S100 Proteins; D004247:DNA