Induced release of Bacillus spores from sporangia by sodium sulphate.
Incubation of sporulating cultures of Bacillus anthracis, B. cereus, B. subtilis and B. thuringiensis in 1.0 mol/l sodium sulphate markedly increased the release of free spores from sporangia. It is postulated that the release of spores is due to activation of latent autolysins which hydrolyse sporangial cell walls. Sodium sulphate-induced lysis of sporangia represents a novel and highly effective method for the recovery of spores from cultures of Bacillus species.
D001407:Bacillus / Q000187:drug effects / Q000502:physiology*; D001408:Bacillus anthracis / Q000187:drug effects / Q000502:physiology; D001409:Bacillus cereus / Q000187:drug effects / Q000502:physiology; D001412:Bacillus subtilis / Q000187:drug effects / Q000502:physiology; D001413:Bacillus thuringiensis / Q000187:drug effects / Q000502:physiology; D013171:Spores, Bacterial; D013431:Sulfates / Q000494:pharmacology*
D013431:Sulfates; C012036:sodium sulfate