Genetic mapping by means of protoplast fusion in Bacillus subtilis.
A new mapping method involving protoplast fusion in Bacillus subtilis is described. Protoplasts from an isogenic standard marker strain containing purA and from a strain containing both purB and the marker, "x", to be mapped were fused with polyethylene glycol, and purA+ purB+ fusants were selected. After isolation of single colonies and determination of unselected markers, marker x was mapped between two standard markers. This method was fully applicable to PBS1-resistant strains (e.g., lyt strains). The results obtained by protoplast fusion, conventional transformation and/or lysed protoplast transformation indicated that a lyt strain, Ni15, contained two new autolysin-minus mutations (lyt-151 and lyt-152). The properties of lyt-15 are also discussed.
D001412:Bacillus subtilis / Q000235:genetics*; D002459:Cell Fusion; D002874:Chromosome Mapping; D005798:Genes, Bacterial; D009068:Movement; D011995:Recombination, Genetic; D014170:Transformation, Genetic