alpha-Difluoromethylornithine enhancement of 14C-putrescine uptake by an androgen-dependent prostatic tumor.
Putrescine is a potential scanning agent for metastases of prostatic carcinoma. We examined the in vivo uptake of [14C]-putrescine by the Dunning R3327H Copenhagen rat prostatic tumor and by other tissues, and conclude that: The uptake of [14C]-putrescine by the tumor was higher than that of the normal dorsolateral prostate, but similar to that of the ventral prostate. Tumor accumulation of [14C]-putrescine was enhanced 38% in intact and 45% in castrated animals by pretreatment with alpha-difluoromethylornithine (DFMO), a polyamine synthesis inhibitor. Further enhancement of tumor uptake (94% in intact and 201% in castrated animals) was achieved by combining DFMO pretreatment with androgen stimulation. Oral administration of methyl-glyoxal bis (guanylhydrazone) (MGBG) increased intestinal uptake of [14C]-putrescine, while oral administration of unlabeled spermine and putrescine decreased it. Dexamethasone decreased the uptake of [14C]-putrescine by the spleen and intestine, but also reduced the prostatic uptake to a considerable extent. These observations are useful for the design of a putrescine-based scan for metastases of prostatic carcinoma.
D000230:Adenocarcinoma / Q000000981:diagnostic imaging*; D000728:Androgens; D000818:Animals; D002250:Carbon Radioisotopes; D003907:Dexamethasone; D013196:Dihydrotestosterone / Q000031:analogs & derivatives; D000518:Eflornithine; D008297:Male; D009376:Neoplasms, Hormone-Dependent / Q000000981:diagnostic imaging*; D011292:Premedication; D011471:Prostatic Neoplasms / Q000000981:diagnostic imaging*; D011700:Putrescine; D011877:Radionuclide Imaging; D051381:Rats
D000728:Androgens; D002250:Carbon Radioisotopes; D013196:Dihydrotestosterone; D003907:Dexamethasone; C030445:dihydrotestosterone propionate; D011700:Putrescine; D000518:Eflornithine