title: Protamine increases the affinity of 3'-phosphoadenosine 5'-phosphosulfate toward a sulfotransferase from chicken embryo epiphyseal cartilage.
abstract: A 3' -phosphoadenosine 5' -phosphosulfate (PAPS):chondroitin sulfate sulfotransferase from chicken embryo epiphyseal cartilage, which was partially purified, exhibited a molecular mass of 150 kDa. The enzymatic sulfation of totally desulfated chondroitin was activated up to 12-fold by protamine while the sulfation of partially sulfated chondroitin was activated only 3-fold. Protamine increased the affinity of the enzyme for PAPS about 4-fold when partially desulfated chondroitin was used as sulfate acceptor. The S 0.5 for the totally desulfated chondroitin was not affected by protamine, while high PAPS concentration slightly increased the affinity of the enzyme for the same sulfate acceptor. The possible role of these substances in the regulation of the sulfation of chondroitin sulfate is discussed.
mesh_terms: D000227:Adenine Nucleotides / Q000378:metabolism*; D000818:Animals; D002642:Chick Embryo; D002807:Chondroitin / Q000031:analogs & derivatives*; D002809:Chondroitin Sulfates / Q000378:metabolism*; D004789:Enzyme Activation / Q000187:drug effects; D007700:Kinetics; D010724:Phosphoadenosine Phosphosulfate / Q000378:metabolism*; D011479:Protamines / Q000494:pharmacology*; D015238:Sulfotransferases; D013466:Sulfurtransferases / Q000378:metabolism*
chemical_list: D000227:Adenine Nucleotides; D011479:Protamines; D010724:Phosphoadenosine Phosphosulfate; D002807:Chondroitin; D002809:Chondroitin Sulfates; C022321:PAPS sulfotransferase; D013466:Sulfurtransferases; D015238:Sulfotransferases