title: An estimate of the extent of deamination of L-serine in auxotrophs of Escherichia coli K-12.
abstract: We have shown that serine-glycine auxotrophs of Escherichia coli K-12 use exogenous L-serine inefficiently as a source of biosynthetic intermediates. Much of the L-serine supplied in the medium is not used to satisfy the auxotrophic requirement, owing to its diversion by L-serine deaminase, presumably to pyruvate. This is the first proof that the activity known as L-serine deaminase actually deaminates L-serine in vivo.
mesh_terms: D003470:Culture Media; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D005998:Glycine / Q000378:metabolism; D012695:L-Serine Dehydratase / Q000378:metabolism*; D009154:Mutation; D012694:Serine / Q000378:metabolism*
chemical_list: D003470:Culture Media; D012694:Serine; D012695:L-Serine Dehydratase; D005998:Glycine