Hematopoietic effects of continuous intravenous infusion of mice with growth factors produced by the WEHI-3 cell line.
A method for continuous intravenous infusion of unanesthetized adult C3H/HeJ or Wx/Wv anemic mice was designed using cage immobilization and a tail vein catheter connected to a model 940 Harvard infusion pump. Infusates included: WEHI-3 cell line dialyzed, 5 times concentrated conditioned medium containing multi-colony-stimulating factor (C-SF) interleukin 3 (IL-3); purified murine IL-3; bacterial endotoxin; serum-free medium, or normal saline. Mice were monitored at days 5-7 after infusion for complete peripheral blood counts and production of granulocytes in vitro by explanted marrow in long-term bone marrow cultures. We observed a stimulatory effect of WEHI-3 conditioned medium infusion that was not attributable to endotoxin and produced significant increases in peripheral blood WBC count and neutrophils, colony-forming units in spleen and numbers of granulocyte/macrophage colony-forming unit culture responsive to both C-SF-1 (L cell C-SF) and multi-C-SF in vitro. This infusion method should prove valuable for test of the in vivo effects of purified growth factors and molecularly cloned hematopoietins.
D000818:Animals; D002460:Cell Line; D003114:Colony-Forming Units Assay; D003115:Colony-Stimulating Factors / Q000008:administration & dosage / Q000494:pharmacology*; D003470:Culture Media / Q000494:pharmacology; D004731:Endotoxins / Q000494:pharmacology; D005260:Female; D006098:Granulocytes / Q000166:cytology; D006410:Hematopoiesis / Q000187:drug effects* / Q000528:radiation effects; D007262:Infusions, Intravenous; D007377:Interleukin-3 / Q000008:administration & dosage / Q000494:pharmacology; D007958:Leukocyte Count; D008264:Macrophages / Q000166:cytology; D008722:Methods; D051379:Mice; D008815:Mice, Inbred Strains / Q000528:radiation effects
D003115:Colony-Stimulating Factors; D003470:Culture Media; D004731:Endotoxins; D007377:Interleukin-3