Simultaneous determination of the rates of synthesis and metabolism of dopamine in various areas of the rat brain: application to the effects of (+)-amphetamine.
A method is described in which two different dopamine turnover estimations are combined. The method is based on coadministration of a DOPA-decarboxylase inhibitor and a monoamine-oxidase inhibitor. This approach allows the simultaneous calculation of the biosynthesis rate of dopamine, as well as the formation rate of its main metabolite 3,4-dihydroxyphenylacetic acid. No mutual interference of the two turnover methods was observed. The method was applied in a dose-effect study of the influence of (+)-amphetamine on the synthesis and metabolism of dopamine.
D015102:3,4-Dihydroxyphenylacetic Acid / Q000032:analysis / Q000378:metabolism; D000661:Amphetamine / Q000494:pharmacology*; D000818:Animals; D001921:Brain / Q000378:metabolism*; D004295:Dihydroxyphenylalanine / Q000032:analysis; D004298:Dopamine / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D006834:Hydrazines / Q000494:pharmacology; D008297:Male; D010293:Pargyline / Q000494:pharmacology; D051381:Rats
D006834:Hydrazines; D015102:3,4-Dihydroxyphenylacetic Acid; D004295:Dihydroxyphenylalanine; D010293:Pargyline; C004297:3-hydroxybenzylhydrazine; D000661:Amphetamine; D004298:Dopamine