Fusion of pro region of subtilisin to staphylococcal protein A and its secretion by Bacillus subtilis.
Subtilisin is synthesized as a preproenzyme in Bacillus subtilis. We fused that region of the subtilisin gene, (apr[BamP]), which encodes the signal sequence and pro region, to the mature gene sequence (spa) for a heterologous protein (staphylococcal protein A). B. subtilis cells harboring this gene fusion synthesized a fusion protein consisting of the signal and pro sequence of subtilisin fused to the protein A; the signal sequence was processed and a fusion protein (pro + protein A) was secreted into the growth medium.
D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000201:enzymology / Q000235:genetics*; D001483:Base Sequence; D004792:Enzyme Precursors / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D005796:Genes; D005798:Genes, Bacterial; D013205:Staphylococcal Protein A / Q000235:genetics*; D013381:Subtilisins / Q000235:genetics*
D004792:Enzyme Precursors; D013205:Staphylococcal Protein A; D013381:Subtilisins; C049752:preprosubtilisin