Influence of cianidanol on specific and non-specific immune mechanisms.
The influence of Cianidanol (Ci), a cytoprotective radical scavenger, on peripheral blood mononuclear cells (PBMC) was assessed with respect to its immunomodulatory function. In previous studies performed in our laboratory, a bidirectional influence of Ci on the immune response was observed, depending on its concentration. In order to elucidate this effect, the influence of Ci on macrophage (M phi) and B-cell function was investigated. A marked dose-dependent suppression of M phi phagocytosis by Ci could be detected. Furthermore, PGE2 synthesis of non-activated and PHA-activated PBMC was inhibited in the presence of Ci. This effect was shown to be due to an inhibition of M phi cyclooxygenase. It was also demonstrated that neither spontaneous nor Staphylococcus aureus Cowan I induced proliferation of highly purified B-cells was enhanced by Ci. Similar results were obtained by measuring the influence of Ci on immunoglobulin secretion of purified B-cells, exposed to Klebsiella membrane preparations. From these data it can be concluded that the previously described enhancing effect of Ci on immunoglobulin secretion is probably T-cell mediated.
D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology; D001578:Benzopyrans / Q000494:pharmacology*; D002392:Catechin / Q000494:pharmacology*; D016861:Cyclooxygenase Inhibitors; D015232:Dinoprostone; D006801:Humans; D007109:Immunity / Q000187:drug effects*; D007136:Immunoglobulins / Q000096:biosynthesis; D066298:In Vitro Techniques; D007213:Indomethacin / Q000494:pharmacology; D008213:Lymphocyte Activation; D008264:Macrophages / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D008934:Mitogens / Q000494:pharmacology; D010587:Phagocytosis / Q000187:drug effects; D011458:Prostaglandins E / Q000096:biosynthesis
D001578:Benzopyrans; D016861:Cyclooxygenase Inhibitors; D007136:Immunoglobulins; D008934:Mitogens; D011458:Prostaglandins E; D002392:Catechin; D015232:Dinoprostone; D007213:Indomethacin