Two Drosophila chorion genes terminate transcription in discrete regions near their poly(A) sites.
We have examined transcription termination of two closely linked Drosophila melanogaster chorion genes, s36-1 and s38-1, using the electron microscope. Our method is unusual and is independent of in vitro nuclear run-on transcription. By measuring transcription unit lengths in chromatin spreads, we can localize efficient termination sites to a region of approximately 210 bp for s36-1 and approximately 365 bp for s38-1. The center of this region is approximately 105 nucleotides downstream of the poly(A) site for the s36-1 gene, and approximately 400 nucleotides downstream for the s38-1 gene. Thus, these two Drosophila chorion genes terminate more closely to their poly(A) addition sites and in a shorter region than many other polyadenylated genes examined to date.
D000818:Animals; D001482:Base Composition; D002823:Chorion; D004331:Drosophila melanogaster / Q000235:genetics*; D005260:Female; D005796:Genes; D011061:Poly A / Q000235:genetics*; D012319:RNA Polymerase II / Q000378:metabolism; D014158:Transcription, Genetic
D011061:Poly A; D012319:RNA Polymerase II