Ethanol induces release of arachidonic acid but not synthesis of eicosanoids in mouse peritoneal macrophages.
Exposure of mouse peritoneal macrophages to ethanol induces a rapid release of arachidonic acid to the extracellular medium. All major classes of phospholipids, phosphatidylcholine, phosphatidylethanolamine and phosphatidylinositol contribute to this release. Ethanol-induced mobilization of arachidonic acid occurs by deacylation, but it is not accompanied by eicosanoid synthesis. These data suggest that at least two signals are necessary for the release and metabolism of arachidonic acid. Ethanol also activates a phospholipase C which hydrolyzes only phosphatidylinositol, and not its phosphorylated derivatives.
D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism*; D000001:Calcimycin / Q000494:pharmacology; D004537:Eicosanoic Acids / Q000096:biosynthesis*; D000431:Ethanol / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D051379:Mice; D010537:Peritoneum / Q000166:cytology; D010766:Phosphorylation
D001095:Arachidonic Acids; D004537:Eicosanoic Acids; D016718:Arachidonic Acid; D000001:Calcimycin; D000431:Ethanol