Studies on pharmacological activation of human serum IgG by chemical modification and active subfragments. V. Mechanism of anti-inflammatory action of carboxamide-methylated L-chain (Fr. I-L) and H-chain (Fr. I-H) from human serum IgG.
Using the xylene ear method in mice, it was demonstrated that the reduced and carboxamide-methylated fragments of human serum IgG (Fr. I-H and Fr. I-L) significantly suppressed capillary permeability. It was also shown that leucocyte emigration and protein leakage into and esterase activity of the pouch fluid, which was accumulated by carboxy-methyl cellulose injections, were suppressed by the administration of the fragments. Moreover, the lipid peroxide level was lowered by both Fr. I-H and Fr. I-L in the liver of either carrageenin-induced, paw edema-bearing or kaolin pouch inflammation in rats. The facts obtained in this study indicated the mechanisms of the anti-inflammatory effects of Fr. I-H and Fr. I-L were mainly caused by both the inhibitory effect on leucocyte emigration into the site of injury and stabilization of the membrane by inhibiting lipid peroxidation of the inflammatory tissue.
D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D000893:Anti-Inflammatory Agents; D002199:Capillary Permeability / Q000187:drug effects; D004950:Esterases / Q000378:metabolism; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism* / Q000627:therapeutic use; D007143:Immunoglobulin Heavy Chains / Q000302:isolation & purification*; D007147:Immunoglobulin Light Chains / Q000302:isolation & purification*; D008054:Lipid Peroxides / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D013482:Superoxide Dismutase / Q000378:metabolism
D000893:Anti-Inflammatory Agents; D007074:Immunoglobulin G; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D008054:Lipid Peroxides; D013482:Superoxide Dismutase; D004950:Esterases; D000135:Acid Phosphatase