Ca2+-dependent K+ transport in lymphocytes.
The treatment of rat thymocytes with A23187 + Ca2+, ascorbate-phenazine methosulphate or propranolol induced quinine-sensitive fluxes of K+ (Rb+) suggesting the presence in the cell membrane of Ca2+-dependent K+ channels. Concanavalin A induced K+ channel activation only at very high doses (13 micrograms/ml). Neither quinine nor the increase of the K+ concentration in the medium to 30 mM prevented the stimulation of amino acid transport induced by concanavalin A, suggesting that the Ca2+-dependent K+ channel is not involved in the early phenomena of lymphocyte activation.
D000818:Animals; D001692:Biological Transport; D000001:Calcimycin / Q000494:pharmacology*; D002118:Calcium / Q000494:pharmacology*; D006801:Humans; D011188:Potassium / Q000378:metabolism*; D011433:Propranolol / Q000494:pharmacology; D011868:Radioisotopes / Q000378:metabolism; D051381:Rats; D012413:Rubidium / Q000378:metabolism; D013601:T-Lymphocytes / Q000378:metabolism*
D011868:Radioisotopes; D000001:Calcimycin; D011433:Propranolol; D012413:Rubidium; D011188:Potassium; D002118:Calcium