Resonance Raman study of reconstituted carotenoproteins incorporating astaxanthin and 15,15'-didehydroastaxanthin.
Two reconstituted carotenoproteins have been studied by resonance Raman spectroscopy. They were prepared from the apoprotein of the Asterias rubens carotenoprotein, asteriarubin and either astaxanthin or 15,15'-didehydroastaxanthin. Spectral properties of dehydrocarotenoids are first discussed. The spectral properties of the complexes are compared to those of the free carotenoids and of other carotenoproteins containing astaxanthin, and possible protein-carotenoid interactions are discussed. Greater delocalisation of the pi-electron system in the central part of the polyene chain, and the role of lateral methyl groups in binding is emphasised.
D000818:Animals; D001059:Apoproteins / Q000302:isolation & purification; D002338:Carotenoids / Q000032:analysis* / Q000302:isolation & purification; D006023:Glycoproteins; D008968:Molecular Conformation; D011485:Protein Binding; D011506:Proteins / Q000032:analysis* / Q000302:isolation & purification; D013059:Spectrum Analysis, Raman; D013215:Starfish / Q000032:analysis; D024341:Xanthophylls; D019207:beta Carotene / Q000031:analogs & derivatives*
D001059:Apoproteins; D006023:Glycoproteins; D011506:Proteins; D024341:Xanthophylls; C052907:asteriarubin protein, Asterias rubens; D019207:beta Carotene; D002338:Carotenoids; C052906:15,15'-didehydroastaxanthin; C005948:astaxanthine