title: Hydrophobic adsorbents for the isolation and purification of biosynthetic human growth hormone from crude fermentation mixtures.
abstract: Hydrophobic interaction chromatography was used as the initial step for the concentration and partial purification of biosynthetic human growth hormone (hGH). This molecule was extracted from the culture medium of a transformed monkey kidney cell line by batch adsorption either to Octyl-Sepharose or Phenyl-Sepharose. The adsorbate was decanted and packed into a column for elution. This initial work-up procedure rendered a concentrated hGH solution that was further purified by ion-exchange and gel filtration chromatography. The hGH obtained was homogeneous, based on polyacrylamide gel electrophoresis, N-terminal sequence and fingerprint analysis.
mesh_terms: D000327:Adsorption; D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D002852:Chromatography, Ion Exchange; D005285:Fermentation; D013006:Growth Hormone / Q000096:biosynthesis / Q000302:isolation & purification*; D000882:Haplorhini; D006801:Humans; D007668:Kidney / Q000166:cytology; D011994:Recombinant Proteins
chemical_list: D011994:Recombinant Proteins; D013006:Growth Hormone