HLA antigens on human hemopoietic progenitors during embryonic-fetal life: expression and in vitro modulation.
We have analyzed the time course of the expression of HLA antigens on hemopoietic progenitors (BFU-E, CFU-E and CFU-GM) from human embryonic-fetal livers (FL). HLA ABC and Ia-like antigens are never detected on progenitor cells at 5-week post-conception. Their expression initiates at 6-week and progressively increases thereafter, up to near-adult levels at 9-week. The time course of HLA antigen expression on erythroid precursors, derived from FL at corresponding gestational ages, parallels that observed on hemopoietic progenitors. Incubation of embryonic progenitors or precursors with medium conditioned by PHA-stimulated adult peripheral blood mononucleated cells induces a marked increase of the expression of both HLA-ABC and Ia-like antigens. Conversely, incubation with recombinant gamma-interferon causes a marked increase of HLA-ABC, but not Ia-like antigen expression, thus in contrast with results observed on adult monocytes.
D000911:Antibodies, Monoclonal; D000937:Antigen-Antibody Reactions; D005865:Gestational Age; D006680:HLA Antigens / Q000032:analysis*; D006681:HLA-D Antigens / Q000276:immunology*; D006684:HLA-DR Antigens / Q000276:immunology*; D006412:Hematopoietic Stem Cells / Q000276:immunology*; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D008099:Liver / Q000196:embryology*
D000911:Antibodies, Monoclonal; D006680:HLA Antigens; D006681:HLA-D Antigens; D006684:HLA-DR Antigens; D007371:Interferon-gamma