Inhibition of endocytosis by anti-clathrin antibodies.
We examined the function of clathrin, a cytoplasmic protein associated with coated pits and vesicles, by introducing monoclonal antibodies into living cells and determining their effects on membrane transport. When anti-clathrin heavy chain antibodies were used, the following effects were observed: clathrin became aggregated in the cytoplasm, the number of coated pits on the plasma membrane was reduced, and adsorbtive endocytosis of Semliki Forest virus and fluid-phase endocytosis were decreased by 40%-50%. No change in transport of newly synthesized influenza hemagglutinin to the plasma membrane was observed. The results indicated that clathrin in CV-1 cells is involved in fluid-phase uptake and receptor-mediated endocytosis, but not in constitutive transport within the secretory pathway.
D000911:Antibodies, Monoclonal; D002966:Clathrin / Q000276:immunology / Q000502:physiology*; D003034:Coated Pits, Cell-Membrane / Q000502:physiology; D004705:Endocytosis; D004721:Endoplasmic Reticulum / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D006389:Hemagglutinins, Viral / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D012672:Semliki forest virus / Q000276:immunology
D000911:Antibodies, Monoclonal; D002966:Clathrin; D006389:Hemagglutinins, Viral; D008565:Membrane Proteins