title: Bone marrow aspirate culture superior to streptokinase clot culture and 8 ml 1:10 blood-to-broth ratio blood culture for diagnosis of typhoid fever.
abstract: We compared the sensitivities of bone marrow aspirate culture (BMAC), 3 ml 1:4 and 8 ml 1:10 blood-to-broth ratio blood cultures (BC), 8 ml streptokinase clot culture (STKCC) and rectal swab culture (RSC) for isolating Salmonella typhi and S. paratyphi A from 61 patients with typhoid or paratyphoid fever in Jakarta, Indonesia. BMAC (92%) was significantly more sensitive than 8 ml BC (62%), 8 ml STKCC (51%), 3 ml BC (44%), RSC (56%) and the 19 ml combination of all three BC methods (71%). The combination of the three BC methods and RSC had an isolation rate of 87%. In Jakarta the diagnosis of typhoid fever cannot be confidently excluded unless a BMAC is done.
mesh_terms: D000293:Adolescent; D000328:Adult; D001769:Blood / Q000382:microbiology; D001853:Bone Marrow / Q000382:microbiology*; D002648:Child; D005260:Female; D006801:Humans; D008297:Male; D010284:Paratyphoid Fever / Q000175:diagnosis* / Q000382:microbiology; D012007:Rectum / Q000382:microbiology; D012482:Salmonella paratyphi A / Q000302:isolation & purification*; D012485:Salmonella typhi / Q000302:isolation & purification*; D018805:Sepsis / Q000382:microbiology; D013300:Streptokinase; D014435:Typhoid Fever / Q000175:diagnosis* / Q000382:microbiology
chemical_list: D013300:Streptokinase