Characterization of protein I from serum-sensitive and serum-resistant transformants of Neisseria gonorrhoeae.
The protein IAs of serum-sensitive (FA635) and serum-resistant (FA638) transformants of Neisseria gonorrhoeae, which have identical pedigrees, have been shown to be different by the use of a monoclonal antibody and were also shown to be different by proteinase K cleavage and primary structural and surface peptide mapping. The difference in structure is within the surface-exposed region of the molecule. The only other difference observed between the two strains was a very slight difference in lipooligosaccharide silver staining in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. These data suggest that protein I alone or in combination with lipooligosaccharide may significantly contribute to serum resistance.
D000942:Antigens, Bacterial / Q000032:analysis*; D001426:Bacterial Proteins / Q000276:immunology*; D008070:Lipopolysaccharides / Q000276:immunology; D008970:Molecular Weight; D009344:Neisseria gonorrhoeae / Q000276:immunology*; D010446:Peptide Fragments / Q000032:analysis
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D008070:Lipopolysaccharides; D010446:Peptide Fragments; C023023:lipid-linked oligosaccharides