title: Bacillus subtilis phage phi 29 main promoters are efficiently recognized in vivo by the Streptomyces lividans RNA polymerase.
abstract: A DNA fragment from the Bacillus subtilis phage phi 29, containing the main early and late viral promoters, has been inserted upstream of the aminoglycoside phosphotransferase gene (neo) derived from the transposon Tn5 and present in a Streptomyces lividans promoter-probe plasmid. The phi 29 promoters are specifically recognized by the S. lividans RNA polymerase which initiates transcription in vivo at the same sites utilized in B. subtilis. Moreover, the viral promoters efficiently direct the synthesis of high levels of the APHII enzyme in S. lividans.
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics; D001435:Bacteriophages / Q000235:genetics*; D003001:Cloning, Molecular; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D010957:Plasmids; D011401:Promoter Regions, Genetic; D013302:Streptomyces / Q000201:enzymology / Q000235:genetics*; D013379:Substrate Specificity; D014158:Transcription, Genetic
chemical_list: D012321:DNA-Directed RNA Polymerases