Cloning and expression of a cDNA encoding a catalytically active fragment of calf thymus DNA polymerase alpha.
A calf thymus cDNA expression library was constructed in the EcoRI site of lambda gt11 and probed with an antibody raised against calf thymus DNA polymerase alpha. Three classes of antibody-reactive clones were isolated. The largest class carried a 1.9 kilobase calf cDNA insert and expressed a 165-175 kilodalton beta-galactosidase:calf fusion protein which displayed DNA polymerase activity. The characteristic responses of the polymerase activity to alpha-specific inhibitors and antibodies identified the 1.9 kilobase cDNA as a sequence specifically derived from the structural gene encoding the pol alpha catalytic core.
D001483:Base Sequence; D002846:Chromatography, Affinity; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis*; D004257:DNA Polymerase II / Q000032:analysis / Q000235:genetics* / Q000276:immunology; D011993:Recombinant Fusion Proteins / Q000032:analysis / Q000302:isolation & purification; D001616:beta-Galactosidase / Q000235:genetics
D011993:Recombinant Fusion Proteins; D004247:DNA; D004257:DNA Polymerase II; D001616:beta-Galactosidase