Cytotoxic factors toward neuroblastoma cells in trypomastigotes of Trypanosoma cruzi.
Homogenates of trypomastigotes of Trypanosoma cruzi (T.c) exhibited low-potency cytotoxic activity toward neuroblastoma cells. The cytotoxic activity was markedly decreased after preservation for 1 week, even at -20 degrees C. Trypsin and pronase E were shown to effectively enhance or restore the cytotoxic activity of T.c by producing some alteration in T.c, depending on concentrations of and treatment time with the enzymes. The cytotoxic factors were insoluble in saline and found in the chloroform extracts of a T.c homogenate. Analysis by thin-layer chromatography showed that the cytotoxic activity of T.c was found in the free fatty acids and lysophospholipids fractions. Of the free fatty acids present in T.c, eicosatetraenoic (20:4) and octadecadienoic (18:2) acids were the most cytotoxic. It was assumed that as much as 27.2% (w/w) of the total lipids of T.c consists of free fatty acids, and 1 mg of protein of the T.c homogenate contains 96 micrograms of free fatty acids. The abundant free fatty acids appear to account for the cytotoxic activity of the T.c homogenate, although they occurred in T.c under weakly active condition.
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D014355:Chagas Disease / Q000097:blood; D002648:Child; D003603:Cytotoxins / Q000494:pharmacology*; D005230:Fatty Acids, Nonesterified / Q000494:pharmacology; D006367:HeLa Cells / Q000166:cytology / Q000187:drug effects; D006801:Humans; D008297:Male; D051379:Mice; D009447:Neuroblastoma / Q000473:pathology*; D011402:Pronase / Q000494:pharmacology; D014349:Trypanosoma cruzi / Q000302:isolation & purification / Q000502:physiology*
D000970:Antineoplastic Agents; D003603:Cytotoxins; D005230:Fatty Acids, Nonesterified; D011402:Pronase