Poly(ADP-ribose) and replicative DNA synthesis studied in permeable mouse thymocytes.
Poly(ADP-ribose) and DNA synthesis were followed in a random population of freshly isolated and permeabilized mouse thymocytes. Different conditions were found to be optimal for the synthesis of poly(ADP-ribose) and DNA, respectively. The suspension of one of the processes did not influence significantly the synthesis of the other macromolecule. Our results suggest that there is no direct relationship between the two processes.
D000818:Animals; D002453:Cell Cycle; D002463:Cell Membrane Permeability; D004261:DNA Replication / Q000187:drug effects; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D008274:Magnesium / Q000494:pharmacology; D051379:Mice; D009702:Nucleoside Diphosphate Sugars / Q000096:biosynthesis*; D011064:Poly Adenosine Diphosphate Ribose / Q000096:biosynthesis*
D009702:Nucleoside Diphosphate Sugars; D011064:Poly Adenosine Diphosphate Ribose; D008274:Magnesium