Synapsin I in PC12 cells. I. Characterization of the phosphoprotein and effect of chronic NGF treatment.
PC12 cells contain a synapsin I-like molecule. Several serum and monoclonal antibodies raised against bovine brain synapsin I bind to and precipitate this molecule, demonstrating immunochemical similarity between the brain and PC12 species. PC12 synapsin I, like brain synapsin I, is a phosphoprotein: It is phosphorylated in intact cells and, when partially purified, serves as a substrate for several synapsin I kinases. PC12 cell synapsin I is structurally similar to brain synapsin I as shown by peptide mapping of 35S-methionine-and 32P-phosphate-labeled molecules from the 2 sources. Chronic NGF treatment of the cells induces a significant increase in the amount of synapsin I relative to total cell protein, measured either by immunolabeling or incorporation of 35S-methionine. The synapsin I present in untreated PC12 cells migrates predominantly as a singlet and that present in cells treated chronically with NGF as a doublet in SDS-PAGE.
D000310:Adrenal Gland Neoplasms / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D011232:Chemical Precipitation; D004591:Electrophoresis, Polyacrylamide Gel; D007158:Immunologic Techniques; D008715:Methionine; D009414:Nerve Growth Factors / Q000494:pharmacology*; D009419:Nerve Tissue Proteins / Q000302:isolation & purification* / Q000378:metabolism; D010673:Pheochromocytoma / Q000378:metabolism; D016704:Synapsins
D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D016704:Synapsins; D008715:Methionine