Isolation and purification of cellular retinol binding protein (CRBP) from goat mammary gland.
The cellular retinol binding protein (CRBP) from goat mammary gland has been isolated in pure form. The molecular weight of CRBP by gel filtration was 15,120 dalton. The 280/350 absorbance ratio of this protein was 1.20 and it gave a single precipitin line on immunodiffusion. The method is very simple and reproducible.
D000818:Animals; D002850:Chromatography, Gel / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006041:Goats; D005779:Immunodiffusion; D007774:Lactation; D008321:Mammary Glands, Animal / Q000378:metabolism*; D008970:Molecular Weight; D011247:Pregnancy; D012177:Retinol-Binding Proteins / Q000302:isolation & purification*; D054840:Retinol-Binding Proteins, Cellular
D012177:Retinol-Binding Proteins; D054840:Retinol-Binding Proteins, Cellular