Chorismate mutase isoenzymes from Sorghum bicolor: immunological characterization.
Highly purified fractions of chorismate mutase 1 and 2 from etiolated seedlings of Sorghum bicolor were used as the antigen for antibody production in BALB/c mice. Tests for antigen-antibody complex formation were made by immunodiffusion, immunoprecipitation, and enzyme-linked immunosorbent assay (ELISA). These tests indicated the presence of specific antibodies for each isoenzyme in their antisera. However, in the same tests, no cross-reaction was found between chorismate mutase 1 and 2 and their antisera. This indicates no immunological similarity between the two isoenzymes of chorismate mutase from sorghum.
D000818:Animals; D000918:Antibody Specificity; D002826:Chorismate Mutase / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D007120:Immunochemistry; D005779:Immunodiffusion; D007527:Isoenzymes / Q000276:immunology*; D007535:Isomerases / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D010944:Plants / Q000201:enzymology*
D007527:Isoenzymes; D007535:Isomerases; D002826:Chorismate Mutase