A human-human monoclonal anti-D by direct fusion with a lymphoblastoid line.
The human lymphoblastoid cell line W1-L2-729-HF2 has been fused with B cells from a plasmaphoresed anti-D donor immunized with D+ cells. A stable monoclonal antibody-producing cell line has been produced which yields culture supernatant of good titre without the need for concentration. The production and use of this reagent as an alternative Rh D typing reagent for use by saline and enzyme manual tests and automated tests in a Technicon 16C machine is discussed. Du red cells are detected in enzyme enhanced tests.
D000888:Antibodies, Anti-Idiotypic / Q000096:biosynthesis; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000302:isolation & purification; D000918:Antibody Specificity; D000921:Antibody-Producing Cells / Q000378:metabolism; D001402:B-Lymphocytes / Q000378:metabolism; D001788:Blood Grouping and Crossmatching; D002459:Cell Fusion; D002460:Cell Line; D003470:Culture Media; D006801:Humans; D007075:Immunoglobulin M / Q000096:biosynthesis; D008854:Microscopy, Electron; D012204:Rh-Hr Blood-Group System
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D003470:Culture Media; D007075:Immunoglobulin M; D012204:Rh-Hr Blood-Group System