Alterations in peptidoglycan of Neisseria gonorrhoeae induced by sub-MICs of beta-lactam antibiotics.
Exposure of Neisseria gonorrhoeae to sub-MICs of selected beta-lactam antibiotics caused distortion of normal cell morphology. Analysis of the peptidoglycan indicated that the cells were accumulating increased quantities of disaccharide pentapeptide in their cell walls. The O-acetylated form of the disaccharide pentapeptide was not detected among the major peaks. The correlation of antibiotic binding to gonococcal penicillin-binding protein 2 and accumulation of non-O-acetylated disaccharide pentapeptide suggested an explanation for the previously observed relationship of penicillin-binding protein 2 and O-acetylation of peptidoglycan.
D000217:Acyltransferases / Q000378:metabolism*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins; D002352:Carrier Proteins; D002473:Cell Wall / Q000187:drug effects / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D004187:Disaccharides / Q000378:metabolism*; D006602:Hexosyltransferases / Q000378:metabolism*; D009097:Multienzyme Complexes / Q000378:metabolism*; D002267:Muramoylpentapeptide Carboxypeptidase; D009344:Neisseria gonorrhoeae / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D009842:Oligopeptides / Q000378:metabolism*; D046915:Penicillin-Binding Proteins; D010457:Peptidoglycan / Q000032:analysis / Q000378:metabolism*; D010458:Peptidyl Transferases / Q000378:metabolism*; D047090:beta-Lactams
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D002352:Carrier Proteins; D004187:Disaccharides; D009097:Multienzyme Complexes; D009842:Oligopeptides; D046915:Penicillin-Binding Proteins; D010457:Peptidoglycan; D047090:beta-Lactams; D000217:Acyltransferases; D010458:Peptidyl Transferases; D006602:Hexosyltransferases; D002267:Muramoylpentapeptide Carboxypeptidase