Characterization of chromosome and plasmid transformation in Bacillus subtilis using gently lysed protoplasts.
Competent cells of Bacillus subtilis were transformed with DNA from gently lysed protoplasts. Significant linkages among markers separated by distances of approximately 2.3% of the total chromosome were found, which have not been detected for conventional transformation. In comparison to previous reports, enhanced plasmid transformation was observed [4.0 X 10(7) transformants per microgram DNA (one transformant per 5 X 10(4) molecules added)], when competent cells were transformed with DNA from lysed protoplasts harboring pUB110.
D001412:Bacillus subtilis / Q000235:genetics*; D002876:Chromosomes, Bacterial; D004269:DNA, Bacterial / Q000235:genetics; D008040:Genetic Linkage; D010957:Plasmids; D011523:Protoplasts; D014169:Transformation, Bacterial
D004269:DNA, Bacterial