title: Evidence against a possible involvement of the serine, and thiol proteases in the exocytotic mechanism of catecholamine secretion in cultured bovine adrenal medullary cells.
abstract: The effects of protease inhibitors on the secretion of catecholamines were studied in cultured bovine adrenal medullary cells. Although the inhibitors of serine proteases could inhibit the carbamylcholine-induced secretion, they failed to inhibit the secretion evoked by either high K+ or A23187. The thiol protease inhibitor had no effect on the secretion. These results therefore seem to indicate that the serine protease inhibitors may inhibit the receptor-mediated secretion probably through their effects on the plasma membrane, thus suggesting that a possible involvement of the serine, and thiol proteases in exocytosis may be unlikely.
mesh_terms: D000313:Adrenal Medulla / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D002217:Carbachol / Q000494:pharmacology; D002395:Catecholamines / Q000378:metabolism*; D002417:Cattle; D002478:Cells, Cultured; D003546:Cysteine Endopeptidases; D010450:Endopeptidases / Q000378:metabolism*; D005089:Exocytosis; D007700:Kinetics; D007976:Leupeptins / Q000494:pharmacology; D009842:Oligopeptides / Q000494:pharmacology; D011188:Potassium / Q000494:pharmacology; D012697:Serine Endopeptidases
chemical_list: D002395:Catecholamines; D007976:Leupeptins; D009842:Oligopeptides; D000001:Calcimycin; D002217:Carbachol; C002101:chymostatin; D010450:Endopeptidases; D012697:Serine Endopeptidases; D003546:Cysteine Endopeptidases; C032854:leupeptin; D011188:Potassium