title: A role for alternative pathway catecholamines in the regulation of steroidogenesis in cow luteal cells.
abstract: Incubation of bovine luteal cells with the alternative pathway catecholamines octopamine, synephrine and deoxyadrenaline at concentrations of 10(-6) to 10(-3) M enhanced the production of progesterone (P less than 0.05). Tryamine did not alter basal progesterone production (P greater than 0.05). Addition of noradrenaline and adrenaline at concentrations of 10(-4) to 10(-7) M significantly elevated the production of progesterone (P less than 0.05). The steroidogenic response to noradrenaline and adrenaline was greater than that for octopamine, synephrine and deoxyadrenaline (P less than 0.05). Response to both primary (10(-6) M) and alternative (10(-4) M) pathway catecholamines was inhibited by propranolol (10(-5) M, P less than 0.05) but not phentolamine (10(-5) M, P greater than 0.05). These results demonstrate that octopamine, synephrine and deoxyadrenaline can affect steroidogenesis by bovine luteal cells, and their action is mediated by beta-adrenergic receptors.
mesh_terms: D000818:Animals; D002417:Cattle; D003338:Corpus Luteum / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D003846:Deoxyepinephrine / Q000494:pharmacology; D005260:Female; D009655:Octopamine / Q000494:pharmacology; D011374:Progesterone / Q000096:biosynthesis*; D013566:Sympathomimetics / Q000494:pharmacology*; D013578:Synephrine / Q000494:pharmacology; D014439:Tyramine / Q000494:pharmacology
chemical_list: D013566:Sympathomimetics; D009655:Octopamine; D011374:Progesterone; D013578:Synephrine; D003846:Deoxyepinephrine; D014439:Tyramine