title: High-efficiency preparative-scale reversed-phase high-performance liquid chromatographic purification of 14C-labelled antibiotics.
abstract: The 14C-labelled antibiotics [2-14C]mupirocin, and [thienyl-3-14C]temocillin cannot be satisfactorily purified on a small scale by conventional methods of chromatography or recrystallisation. Their purification was successfully achieved by high-efficiency preparative-scale reversed-phase high-performance liquid chromatography. The purifications employed 250 mm X 10 mm I.D. or 22 mm I.D. stainless-steel columns packed with Merck LiChrosorb RP-18 (10 microns) stationary phase which were eluted with aqueous buffer solutions at flow-rates of 10-25 ml min-1 using conventional analytical instrumentation.
mesh_terms: D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D005227:Fatty Acids / Q000032:analysis; D007202:Indicators and Reagents; D008968:Molecular Conformation; D016712:Mupirocin; D010406:Penicillins / Q000032:analysis
chemical_list: D000900:Anti-Bacterial Agents; D005227:Fatty Acids; D007202:Indicators and Reagents; D010406:Penicillins; C031367:temocillin; D016712:Mupirocin