The production and use of monoclonal antibodies to measure apolipoprotein B by a competitive enzyme-linked immunoassay.
The production of monoclonal antibodies to human lipoproteins is described. One of these antibodies, which was shown to be specific to apolipoprotein B, was used to develop a competitive enzyme-linked immunoassay for apolipoprotein B in serum samples. The antibody selected recognises apolipoprotein B in both low density and very low density lipoprotein particles, but there is no cross-reactivity with high density lipoprotein. There is no requirement for labelling of antigen or antibody used in the assay, and results obtained correlate well (r = 0.88) with measurements on serum samples using a radial immunodiffusion assay for apolipoprotein B.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000096:biosynthesis*; D000918:Antibody Specificity; D001055:Apolipoproteins B / Q000032:analysis*; D001161:Arteriosclerosis / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D005779:Immunodiffusion; D007202:Indicators and Reagents; D008074:Lipoproteins / Q000032:analysis; D008075:Lipoproteins, HDL / Q000032:analysis; D008077:Lipoproteins, LDL / Q000032:analysis; D008079:Lipoproteins, VLDL / Q000032:analysis; D051379:Mice
D000911:Antibodies, Monoclonal; D001055:Apolipoproteins B; D007202:Indicators and Reagents; D008074:Lipoproteins; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D008079:Lipoproteins, VLDL