Rat heart cell membranes contain three substrates for cholera toxin-catalyzed ADP-ribosylation and a single substrate for pertussis toxin-catalyzed ADP-ribosylation.
Three peptides (Mr = 45,000, 47,000 and 52,000) in the cholate extract from rat heart cell membranes were radiolabeled when the extract was incubated in the presence of activated cholera toxin and [32P]NAD. A single peptide of Mr = 41,000 in this extract was ADP-ribosylated by pertussis toxin in the presence of [32P]NAD.
D000246:Adenosine Diphosphate Ribose / Q000378:metabolism*; D000818:Animals; D002772:Cholera Toxin / Q000378:metabolism*; D019204:GTP-Binding Proteins / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D008970:Molecular Weight; D009206:Myocardium / Q000378:metabolism*; D009702:Nucleoside Diphosphate Sugars / Q000378:metabolism*; D037342:Pertussis Toxin; D051381:Rats; D012508:Sarcolemma / Q000378:metabolism; D013379:Substrate Specificity; D010566:Virulence Factors, Bordetella / Q000378:metabolism*
D008565:Membrane Proteins; D009702:Nucleoside Diphosphate Sugars; D010566:Virulence Factors, Bordetella; D000246:Adenosine Diphosphate Ribose; D002772:Cholera Toxin; D037342:Pertussis Toxin; D019204:GTP-Binding Proteins