Determination of ADP-ribosyl arginine anomers by reverse-phase high-performance liquid chromatography.
A simple and rapid method for the determination of ADP-ribosyl arginine anomers was devised. Analysis is performed by reverse-phase high-performance liquid chromatography on a 5-micron Cosmosil 5C18 column and uv detection. ADP-ribosylation of arginine by hen liver ADP-ribosyl-transferase and the effect of pH on anomerization are also presented.
D036002:ADP Ribose Transferases; D000246:Adenosine Diphosphate Ribose / Q000031:analogs & derivatives* / Q000032:analysis; D000818:Animals; D002645:Chickens; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008099:Liver / Q000201:enzymology; D010430:Pentosyltransferases; D013237:Stereoisomerism
D000246:Adenosine Diphosphate Ribose; C043357:ADP-ribosylarginine; D036002:ADP Ribose Transferases; D010430:Pentosyltransferases