title: Murine hematopoietic cells with pre-B or pre-B/myeloid characteristics are generated by in vitro transformation with retroviruses containing fes, ras, abl, and src oncogenes.
abstract: In vitro infection of bone marrow or fetal liver cells with retroviruses containing fes, abl, ras, or src oncogenes resulted in the transformation of early B lineage cells. All cell lines tested possessed rearrangements at the Ig heavy chain locus and some had rearrangements at the K chain locus. The majority of the lines corresponded phenotypically to Lyb-2+, Ly-5(B220)+, ThB- large pre-B cells, although some were classified as pro-B cells because of their Lyb-2+, Ly-17+, Ly-5(B220)- phenotype. We identified two cell lines that contained subpopulations of cells that coexpressed the B lineage antigens Lyb-2 and Ly-5(B220) and the myeloid lineage antigen Mac-1. Single-cell FMF cloning of these subpopulations showed that Mac-1+ cells were derived from Mac-1- cells and that these Mac-1+-cloned cells further differentiated into cells with phenotypic and functional characteristics of mature macrophages.
mesh_terms: D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D001402:B-Lymphocytes / Q000145:classification / Q000276:immunology*; D002460:Cell Line; D002469:Cell Separation; D002472:Cell Transformation, Viral; D006412:Hematopoietic Stem Cells / Q000145:classification / Q000276:immunology*; D007144:Immunoglobulin J-Chains / Q000235:genetics; D008213:Lymphocyte Activation; D016177:Macrophage-1 Antigen; D051379:Mice; D008815:Mice, Inbred Strains; D009857:Oncogenes; D010641:Phenotype; D012190:Retroviridae / Q000235:genetics / Q000276:immunology*
chemical_list: D000954:Antigens, Surface; D007144:Immunoglobulin J-Chains; D016177:Macrophage-1 Antigen