Cycloheximide reduces PGD2 or delta 12-PGJ2 cytotoxicity on NCG cells.
To study the precise mechanism of cytotoxic activity of PGD2 or delta 12-PGJ2 (a biologically active metabolite of PGD2), we examined the effect of various compounds on PGD2 or delta 12-PGJ2 cytotoxicity, using a human neuroblastoma cell line (NCG). Cycloheximide (CHM) specifically protected PGD2 cytotoxicity on NCG cells. When delta 12-PGJ2 was tested, CHM exhibited a similar rescue effect. Puromycin, mitomycin C, and alpha-amanitin did not affect PGD2 or delta 12-PGJ2 cytotoxicity. Emetine showed a variable and no consistent rescue effect CHM may have been active at the primary site where PGD2 or delta 12-PGJ2 exerts its cytotoxicity. This is the first report indicating that CHM reduces the cytotoxicity induced by PGD2 or delta 12-PGJ2.
D000546:Amanitins / Q000494:pharmacology; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003513:Cycloheximide / Q000494:pharmacology*; D004347:Drug Interactions; D004640:Emetine / Q000494:pharmacology; D006801:Humans; D016685:Mitomycin; D008937:Mitomycins / Q000494:pharmacology; D009447:Neuroblastoma / Q000188:drug therapy*; D015230:Prostaglandin D2 / Q000031:analogs & derivatives; D011457:Prostaglandins D / Q000494:pharmacology*; D011691:Puromycin / Q000494:pharmacology
D000546:Amanitins; D008937:Mitomycins; D011457:Prostaglandins D; D011691:Puromycin; D016685:Mitomycin; C040810:9-deoxy-9,10-didehydro-12,13-didehydro-13,14-dihydroprostaglandin D2; D003513:Cycloheximide; D015230:Prostaglandin D2; D004640:Emetine