Regulation of IL 2 expression in mitogen-activated murine T lymphocytes.
In mitogen-stimulated mouse spleen cells, the IL 2 gene is only transiently expressed with maximal mRNA steady state levels between 6-14 h post stimulation with Concanavalin A (Con A). This is also reflected by the kinetics of IL 2 release into culture supernatants. Con A-stimulated L3T4+ and Lyt2+ T cell subpopulations express the IL 2 gene and produce IL 2 similarly. The half-life of IL 2 mRNA is only 30 min, but can be prolonged significantly by cycloheximide. At later post stimulation times IL 2 gene transcription is reduced, as indicated by the reduced effect of cycloheximide. IL 2 gene expression is not influenced by added IL 2 or IFN-gamma.
D000818:Animals; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D003513:Cycloheximide / Q000494:pharmacology; D004987:Ethers / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects; D007371:Interferon-gamma / Q000494:pharmacology; D007376:Interleukin-2 / Q000096:biosynthesis* / Q000235:genetics; D015759:Ionomycin; D007700:Kinetics; D051379:Mice; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D004987:Ethers; D007376:Interleukin-2; D012333:RNA, Messenger; D003208:Concanavalin A; D015759:Ionomycin; D007371:Interferon-gamma; D003513:Cycloheximide; D013755:Tetradecanoylphorbol Acetate