Guanine nucleotide regulation of the pertussis and cholera toxin substrates of rat glioma C6 BU1 cells.
Rat glioma C6 BU1 cells contain a pertussis toxin substrate of 40 kDa which does not appear to be identical with Gi,Go or transducin. The GTP analogue, GTP[gamma S], inhibited the rate of pertussis toxin-catalysed ADPribosylation of this protein, while the GDP analogue GDP[beta S] stimulated this reaction. A protein of the same kDa value was ADPribosylated by cholera toxin in the absence of added guanine nucleotides. It is suggested that this 40 kDa protein can be a substrate for both cholera and pertussis toxins under appropriate conditions.
D000246:Adenosine Diphosphate Ribose / Q000378:metabolism*; D000818:Animals; D002772:Cholera Toxin / Q000378:metabolism*; D019204:GTP-Binding Proteins / Q000378:metabolism*; D005910:Glioma / Q000378:metabolism*; D006150:Guanine Nucleotides / Q000502:physiology*; D008970:Molecular Weight; D009243:NAD / Q000378:metabolism; D037342:Pertussis Toxin; D051381:Rats; D010566:Virulence Factors, Bordetella / Q000378:metabolism*
D006150:Guanine Nucleotides; D010566:Virulence Factors, Bordetella; D009243:NAD; D000246:Adenosine Diphosphate Ribose; D002772:Cholera Toxin; D037342:Pertussis Toxin; D019204:GTP-Binding Proteins