Thin layer chromatographic confirmation of aflatoxin M1 extracted from milk.
Aflatoxin M1 can be confirmed directly on a thin layer plate by reacting the toxin with a mixture of reagents containing p-anisaldehyde. This confirmatory procedure requires only 2 elutions in the same direction using 2 different solvents. The mixture containing p-anisaldehyde is overspotted on M1 after the plate has been developed in toluene-ethyl acetate-ethyl ether-formic acid (25 + 35 + 40 + 5). The plate is heated at 110 degrees C for 10 min and then developed in hexane-acetone-chloroform (15 + 50 + 35). The Rf value of the green fluorescent derivative is less than that of the M1 standard. This confirmatory procedure requires only one-dimensional TLC, so several sample extracts and the standard can be run simultaneously. The minimum detectable quantity of aflatoxin M1 on the TLC plate with this test is 0.3 ng. p-Anisaldehyde reagent solution may also be used as a spray reagent for the confirmation of aflatoxin M1. The procedures described were satisfactory for confirming the mycotoxin in spiked samples of powdered and liquid milk.
D016607:Aflatoxin M1; D000348:Aflatoxins / Q000302:isolation & purification*; D000818:Animals; D002417:Cattle; D002855:Chromatography, Thin Layer / Q000379:methods; D005506:Food Contamination / Q000032:analysis*; D007202:Indicators and Reagents; D008892:Milk / Q000032:analysis*
D000348:Aflatoxins; D007202:Indicators and Reagents; D016607:Aflatoxin M1