[Allelic exclusion in hybridomas. II. Characteristics of the synthesis of 2 allelic variants of rat immunoglobulin kappa-chains in a cloned hybridoma line].
Production of Igk-1a and Igk-1b allelic variants of rat Ig kappa-chains in hybridoma clone 4C2 was studied. Gel-filtration of the culture fluid demonstrates that the cells secrete isolated L-chains in monomeric and dimeric form. Different L-chain allotypes fail to associate in dimers. Immunofluorescence studies prove that both allelic variants are produced in a single cell. Cells with double allotype production loose the ability to produce one or both allotypes at an essentially heightened rate as compared to those producing Igk-1a or Igk-1b alone. The results fit well into the regulated allelic exclusion model.
D000483:Alleles; D000818:Animals; D003001:Cloning, Molecular; D006825:Hybridomas; D007126:Immunoglobulin Allotypes / Q000096:biosynthesis / Q000235:genetics*; D007145:Immunoglobulin kappa-Chains / Q000096:biosynthesis / Q000235:genetics*; D051381:Rats
D007126:Immunoglobulin Allotypes; D007145:Immunoglobulin kappa-Chains
Allel'noe iskliuchenie v gibridomakh. II. Osobennosti sinteza dvukh allel'nykh variantov kappa-tsepeĭ immunoglobulinov krysy klonirovannoĭ gibridomnoĭ linieĭ.