An ultra-rapid method for the study of antibiotic resistance plasmids.
A rapid method of isolating plasmid DNA has been developed from that of Birnboim & Doly (1979). This method allows large numbers of strains to be examined, and can be employed to isolate DNA from members of the Enterobacteriaceae, Pseudomonas aeruginosa, Staphylococcus aureus, (including methicillin-resistant strains) and coagulase-negative staphylococci. Plasmids of widely differing sizes are amenable to isolation by this technique, which yields DNA of sufficient purity to allow restriction endonuclease and homoduplex analysis.
D001419:Bacteria / Q000235:genetics*; D004269:DNA, Bacterial / Q000032:analysis*; D004587:Electrophoresis, Agar Gel; D004755:Enterobacteriaceae / Q000235:genetics; D011550:Pseudomonas aeruginosa / Q000235:genetics; D011815:R Factors; D013211:Staphylococcus aureus / Q000235:genetics
D004269:DNA, Bacterial