A method to average immunofluorescent histograms.
Single parameter gated flow cytometric fluorescent histograms were obtained on normal donor blood mononuclear cells using several commonly available lymphocyte surface markers. A computer method was developed to average single parameter flow cytometric immunofluorescent histograms. The averaged histograms provide a means of pattern recognition for normal lymphocytes and will aid in the clinical evaluation of lymphocytosis. Averaged histograms may also serve as standards for more advanced analysis.
D005434:Flow Cytometry / Q000379:methods*; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D006801:Humans; D008214:Lymphocytes / Q000145:classification*; D013861:Thiocyanates / Q000378:metabolism
D005452:Fluoresceins; D013861:Thiocyanates; D016650:Fluorescein-5-isothiocyanate