Studies on pristinamycin synergism in Staphylococcus aureus.
Binding experiments were performed with both components of the pristinamycin complex (pristinamycin IA (PIA) and pristinamycin IIA (PIIA] using ribosomes from sensitive and resistant Staphylococcus aureus. Fluorescence polarization was used to measure PIA binding. The results obtained show a direct correlation between inhibition, synergy and the enhancement of the affinity of PIA for its receptor in the presence of PIIA. The uptake of PIA by intact cells seems to be directly correlated with affinity between PIA and ribosomes, a phenomenon which is probably shared with the macrolide antibiotics.
D000900:Anti-Bacterial Agents / Q000378:metabolism*; D004357:Drug Synergism; D005454:Fluorescence Polarization; D010455:Peptides / Q000378:metabolism / Q000494:pharmacology; D012270:Ribosomes / Q000378:metabolism; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000378:metabolism; D014316:Tritium; D014769:Virginiamycin / Q000378:metabolism
D000900:Anti-Bacterial Agents; D010455:Peptides; D014316:Tritium; D014769:Virginiamycin