Periodate oxidation of glycolipids: a borohydride-periodate-Schiff method for ganglioside demonstration in tissue sections.
Among lipids, gangliosides can be selectively stained with Schiff's reagent if the oxidizing agent (sodium metaperiodate) is sufficiently dilute to exclude all but the readily oxidized sialic acid sugars. A borohydride-periodate-Schiff (BhPS) sequence is recommended as a reliable method, convenient to perform, for the detection of the intraneuronal lipid accumulations in the ganglioside storage disorders.
D001894:Borohydrides; D001923:Brain Chemistry; D005677:G(M1) Ganglioside; D005732:Gangliosides / Q000032:analysis*; D005733:Gangliosidoses / Q000378:metabolism*; D006651:Histocytochemistry; D006801:Humans; D010084:Oxidation-Reduction; D010503:Periodic Acid-Schiff Reaction; D013116:Spinal Cord / Q000032:analysis*; D013661:Tay-Sachs Disease / Q000378:metabolism
D001894:Borohydrides; D005732:Gangliosides; D005677:G(M1) Ganglioside; C025364:sodium borohydride