Metabolic and physiological consequences of the effect of phenylhydrazonopropanedinitriles on Candida albicans.
Phenylhydrazonopropanedinitrile, a model uncoupler of oxidative phosphorylation, was used in studies of metabolic and physiological consequences of uncoupling at the cellular level in Candida albicans. Concentrations stimulating respiration induce a faster glucose consumption at a practically unchanged respiratory coefficient. The extracellular production of acids is also without significant changes. When applying higher concentrations of the uncoupler respiration was inhibited, similarly to glucose consumption and acid production. This fact is due to nonspecific interactions of the alkylation type with mercapto groups of functional proteins. Phenylhydrazonopropanedinitrile influences energy-generating processes resulting in slowing down or interruption of biosynthetic processes and occasionally even growth of Candida albicans.
D002176:Candida albicans / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D002245:Carbon Dioxide / Q000378:metabolism; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000031:analogs & derivatives / Q000494:pharmacology*; D005947:Glucose / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D009570:Nitriles / Q000494:pharmacology*; D010085:Oxidative Phosphorylation / Q000187:drug effects*; D010101:Oxygen Consumption / Q000187:drug effects*; D014475:Uncoupling Agents / Q000494:pharmacology*
D009570:Nitriles; D014475:Uncoupling Agents; D002245:Carbon Dioxide; C012804:carbonyl cyanide phenylhydrazone; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D005947:Glucose