[Improved culture of Corynebacterium diphtheriae for obtaining toxin-anatoxin].
The work deals with the optimization of the cultivation of C. diphtheriae with a view to obtaining diphtheria toxin--toxoid on the basis of the study of the kinetics of microbial growth and toxin formation. The combined cultivation process consisting of 3 cycles has been experimentally developed and realized under industrial conditions. The use of this cultivation method has made it possible to obtain more balanced cultures and to standardize the biological properties of the biomass and toxin thus obtained. The characteristic features of the toxoid preparations obtained on the basis of this method are a high degree of purification, good immunogenic properties and constant fractional composition; their degree of homogeneity approximates the molecular homogeneous state.
D000818:Animals; D001431:Bacteriological Techniques; D003353:Corynebacterium diphtheriae / Q000254:growth & development*; D003470:Culture Media / Q000378:metabolism; D004167:Diphtheria Toxin / Q000096:biosynthesis / Q000302:isolation & purification* / Q000633:toxicity; D004168:Diphtheria Toxoid / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification*; D006168:Guinea Pigs; D007700:Kinetics; D013997:Time Factors
D003470:Culture Media; D004167:Diphtheria Toxin; D004168:Diphtheria Toxoid
Sovershenstvovanie kul'tivirovaniia difteriĭnykh korinebakteriĭ dlia polucheniia toksina-anatoksina.