title: Expression of epidermis-specific antigens during embryogenesis of the ascidian, Halocynthia roretzi.
abstract: We have produced two monoclonal antibodies (Epi-1 and Epi-2) which specifically recognize epidermal cells and their derivative, the larval tunic, of developing embryos of the ascidian Halocynthia roretzi. The antigens, examined by indirect immunofluorescence staining, first appear at the early tailbud stage and are present until at least the swimming larval stage. There were distinct and separate puromycin and actinomycin D sensitivity periods for each antigen. Aphidicolin, a specific inhibitor of DNA synthesis, prevented the appearance of each antigen when embryos were exposed to the drug continuously from cleavage stages. These results suggest that the antigens are synthesized during embryogenesis by developing epidermal cells and that several rounds of DNA replication are required for the antigen expression. Early cleavage stage embryos, including fertilized but unsegmented eggs, in which cytokinesis had been blocked with cytochalasin B expressed the antigens, and blastomeres exhibiting the antigens were always of the epidermis lineage. In partial embryos produced by four separated blastomere pairs of the 8-cell embryos, the expression of antigens was seen only in those developed from the animal blastomere pairs, which are progenitors of epidermal cells. These observations indicate that differentiation of epidermal cells in ascidian embryos takes place in a typical "mosaic" fashion.
mesh_terms: D000818:Animals; D000941:Antigens / Q000032:analysis* / Q000235:genetics; D016590:Aphidicolin; D001757:Blastomeres / Q000166:cytology; D002454:Cell Differentiation; D004261:DNA Replication / Q000187:drug effects; D003609:Dactinomycin / Q000494:pharmacology; D004224:Diterpenes / Q000494:pharmacology; D000078404:Epidermal Cells; D005455:Fluorescent Antibody Technique; D014176:Protein Biosynthesis / Q000187:drug effects; D011691:Puromycin / Q000494:pharmacology; D014158:Transcription, Genetic; D014561:Urochordata / Q000196:embryology* / Q000276:immunology
chemical_list: D000941:Antigens; D004224:Diterpenes; D003609:Dactinomycin; D016590:Aphidicolin; D011691:Puromycin