A novel agglutination test for the human immunodeficiency virus antibody: a comparative study with enzyme-linked immunosorbent assay and immunofluorescence.
A new serological test, the gelatin particle agglutination (PA) test, was developed for the detection of antibody to human immunodeficiency virus (HIV). The sensitivity of the PA test was the same as that of enzyme-linked immunosorbent assay (ELISA) among 761 US and Japanese sera tested. However, false-positive cases were found in 1.2% of all sera in ELISA but in 0% in the PA test (P less than 0.05). There was 100% agreement of seropositivity between PA and a confirmatory indirect immunofluorescence (IF) test. In addition, the agglutination test was reproducible. Thus, the test should be useful for the mass screening of anti-HIV antibody in blood donors.
D000163:Acquired Immunodeficiency Syndrome / Q000382:microbiology; D000372:Agglutination Tests; D000914:Antibodies, Viral / Q000032:analysis*; D001782:Blood Donors; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006678:HIV / Q000276:immunology*; D015483:HIV Antibodies; D006801:Humans
D000914:Antibodies, Viral; D015483:HIV Antibodies