Enumeration of the T4 positive and T8 positive lymphocytes in AIDS and related syndromes. Comparison of the immunogold and the immunofluorescence techniques.
Immunofluorescence and immunogold techniques yielded similar results when used to determine the T lymphocyte subsets (T4 and T8 positive cells) in patients with AIDS or AIDS-related complex and in healthy homosexual men seropositive for HTLV III/LAV antibody. In these pathological situations, the advantages of immunogold staining could render this technique useful in a clinical laboratory.
D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology*; D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D006046:Gold; D006716:Homosexuality; D006801:Humans; D007158:Immunologic Techniques; D007958:Leukocyte Count; D008206:Lymphatic Diseases / Q000276:immunology*; D008297:Male; D013601:T-Lymphocytes / Q000145:classification* / Q000276:immunology / Q000382:microbiology
D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface; D006046:Gold