Bone tissue response to irradiation and treatment model of mandibular irradiation injury. An experimental and clinical study.
The present study was conducted on bone tissue responses to irradiation towards a treatment model of mandibular irradiation injury by comparing the results of experimental observations of irradiation effects on rabbit hind legs and rat mandibular bones (paper I, II and III) with clinical observations of irradiation effects on the human mandible (paper IV, V and VI). The main results of the study were as follows: Bone marrow haemorrhage, eosinophilia and incipient edema were encountered in the rabbit leg one day after a single irradiation dose. Edema and fibrosis were the salient features after five weeks, while both regenerative and fibrotic changes predominated eleven weeks after irradiation. The changes were the more extensive the greater the irradiation dose was. Empty lacunae as a sign of cell damage in cortical bone already appeared on the first day after irradiation; this effect reached its maximum when the dose was 20 Gy or more. Bone marrow and subcutaneous tissue pO2 and pCO2 were measured by means of implanted Silastic tonometers in irradiated and nonirradiated rabbit hind legs. Single dose irradiation was followed by a rapid, dose dependent decrease of marrow pO2. The corresponding effect on pCO2 was weaker and appeared later. The response to hyperoxia in the bone marrow became weaker when the irradiation dose increased. Less significant was the response of CO2 tension to hyperoxia. O2 and CO2 tensions were recovered after single dose irradiation both in subcutaneous tissue and in bone marrow, but the reduction was less in bone marrow. During the twelve weeks observation period clearly better recovery in tissue gas tensions was observed in subcutaneous tissue than in bone marrow. Nonirradiated periosteal grafts on irradiated bone cavities in the rabbit tibia induced more rapid and intense mature bone formation than irradiated periosteal grafts. The irradiated periosteum, even after a single dose of 20 Gy, had some osteogenetic capacity. The alkaline phosphatase content was lowered eight weeks after surgery in irradiated legs but clearly exceeded control values twelve weeks after surgery indicating new bone formation. Lysosomal enzyme DAP II contents were increased in all irradiated specimens as a sign of disturbed bone formation. The tissue concentrations of acid phosphatase, cytochrome oxidase, lactate dehydrogenase, isocitrate dehydrogenase, glucose-6-phosphate dehydrogenase and succinate dehydrogenase in the immediate postirradiation period showed a greater increase in activity in the cut lines of the irradiated rat mandibles than in those of the nonirradiated mandibles.(ABSTRACT TRUNCATED AT 400 WORDS)
D000328:Adult; D000818:Animals; D001853:Bone Marrow / Q000528:radiation effects; D001861:Bone Regeneration / Q000528:radiation effects; D002245:Carbon Dioxide / Q000378:metabolism; D003238:Connective Tissue / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D004798:Enzymes / Q000378:metabolism; D005260:Female; D006258:Head and Neck Neoplasms / Q000532:radiotherapy*; D006801:Humans; D008297:Male; D008334:Mandible / Q000000981:diagnostic imaging / Q000528:radiation effects*; D008875:Middle Aged; D010019:Osteomyelitis / Q000628:therapy; D010025:Osteoradionecrosis / Q000000981:diagnostic imaging / Q000628:therapy*; D010101:Oxygen Consumption / Q000528:radiation effects; D010521:Periosteum / Q000528:radiation effects / Q000637:transplantation; D011817:Rabbits; D011832:Radiation Injuries / Q000628:therapy*; D011877:Radionuclide Imaging; D013977:Tibia / Q000528:radiation effects
D004798:Enzymes; D002245:Carbon Dioxide