Effect of cord factor, a toxic glycolipid from Mycobacterium tuberculosis, on mouse liver drug metabolizing enzymes.
Cord factor (a mycobacterial toxin) treatment of mice for 72 hr resulted in decreased activities of hepatic drug metabolizing enzymes. The toxin treated animals exhibited reduced levels of liver cytochrome P-450 and cytochrome b5, accompanied by significant lowering of NADPH-cytochrome c reductase and NADH-cytochrome b5 reductase activities. The hepatic activities of aminopyrine N-demethylase and aniline hydroxylase were diminished, while liver cytosolic glutathione S-transferase activity was inhibited in mice receiving the toxin. Earlier studies from this laboratory (J. K. Batra, Ph.D. Thesis, Delhi University, India, 1982) on the effects of experimental tuberculosis on hepatic drug metabolism revealed changes similar to the presently reported influence of cord factor on mouse liver microsomal monooxygenases. Thus, the action of cord factor (on hepatic drug metabolism) largely mimics the effects of tuberculosis infection.
D000818:Animals; D001427:Bacterial Toxins / Q000633:toxicity*; D003311:Cord Factors / Q000633:toxicity*; D003577:Cytochrome P-450 Enzyme System / Q000032:analysis; D006017:Glycolipids / Q000633:toxicity*; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D051379:Mice; D008862:Microsomes, Liver / Q000187:drug effects; D008930:Mitochondria, Liver / Q000187:drug effects; D009169:Mycobacterium tuberculosis / Q000032:analysis*; D004364:Pharmaceutical Preparations / Q000378:metabolism*
D001427:Bacterial Toxins; D003311:Cord Factors; D006017:Glycolipids; D004364:Pharmaceutical Preparations; D003577:Cytochrome P-450 Enzyme System