Molecular characterization of the interferon-induced 15-kDa protein. Molecular cloning and nucleotide and amino acid sequence.
We have isolated a cDNA clone for an interferon-induced 15-kDa protein. The cDNA clone was prepared from mRNA isolated from interferon-beta-treated human Daudi cells. The clone of 635 base pairs contains an open reading frame coding for a protein of 145 amino acids, and suggests for the mRNA a 75-base pair 5' untranslated and a 125-base pair 3' untranslated region. Approximately 85% of the amino acid sequence of the 15-kDa protein has been independently obtained from 2 nmol of material using microsequencing technology on the N terminus of the intact protein and on tryptic and chymotryptic peptides. The amino acid sequence of the isolated protein is identical to the amino acid sequence deduced from the cDNA. Northern blot analysis confirmed that the mRNA for the 15-kDa protein is undetectable in untreated cells, but is greatly induced following interferon treatment.
D000595:Amino Acid Sequence; D001483:Base Sequence; D002051:Burkitt Lymphoma; D002460:Cell Line; D002918:Chymotrypsin; D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification*; D005796:Genes / Q000187:drug effects*; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D008970:Molecular Weight; D010446:Peptide Fragments / Q000032:analysis; D011506:Proteins / Q000032:analysis*; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification; D014357:Trypsin
D010446:Peptide Fragments; D011506:Proteins; D012333:RNA, Messenger; D007371:Interferon-gamma; D004247:DNA; D002918:Chymotrypsin; D014357:Trypsin