Analysis of immunoglobulins expressing the V kappa 21E and V kappa 21D gene products. Evidence for multigenic control of the expression of VH-VL combinations.
The aim of this work was to study the genetic control of VH-VL combined expression. To this end immunoglobulins expressing the V kappa 21E and V kappa 21D gene products were isolated from the normal sera of several inbred strains of mice using a monoclonal antibody that selectively reacts with V kappa 21E and V kappa 21D subgroups. Analysis of the isoelectric focusing (IEF) pattern of the IgG heavy chains of these immunoglobulins obtained from H-2-congeneic mice revealed as expected that Igh-linked loci strongly influence VH-VL combined expression. The IEF pattern of V kappa 21D and V kappa 21E-associated heavy chains isolated from recombinant inbred mouse strains, however, revealed different phenotypes from either parental strains in strains in which both H-2 and Igh loci segregated from the same parent. These data, therefore, strongly suggest that the expression of VH-VL combinations is controlled in part by genetic loci which segregate independently from those linked to H-2 and Igh.
D000818:Animals; D000911:Antibodies, Monoclonal; D005786:Gene Expression Regulation; D005796:Genes; D006183:H-2 Antigens / Q000235:genetics; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D007142:Immunoglobulin gamma-Chains / Q000235:genetics*; D007145:Immunoglobulin kappa-Chains / Q000235:genetics*; D007526:Isoelectric Point; D051379:Mice; D008815:Mice, Inbred Strains; D011336:Probability; D011863:Radioimmunoassay
D000911:Antibodies, Monoclonal; D006183:H-2 Antigens; D007143:Immunoglobulin Heavy Chains; D007135:Immunoglobulin Variable Region; D007142:Immunoglobulin gamma-Chains; D007145:Immunoglobulin kappa-Chains