Cloning of a human liver microsomal UDP-glucuronosyltransferase cDNA.
A cDNA clone (HLUG 25) encoding the complete sequence of a human liver UDP-glucuronosyltransferase was isolated from a lambda gt11 human liver cDNA library. The library was screened by hybridization to a partial-length human UDP-glucuronosyltransferase cDNA (pHUDPGT1) identified from a human liver pEX cDNA expression library by using anti-UDP-glucuronosyltransferase antibodies. The authenticity of the cDNA clone was confirmed by hybrid-select translation and extensive sequence homology to rat liver UDP-glucuronosyltransferase cDNAs. The sequence of HLUG 25 cDNA was determined to be 2104 base-pairs long, including a poly(A) tail, and contains a long open reading frame. The possible site of translation initiation of this sequence is discussed with reference to a rat UDP-glucuronosyltransferase cDNA clone (RLUG 38).
D000818:Animals; D000906:Antibodies / Q000032:analysis; D001483:Base Sequence; D003001:Cloning, Molecular; D003062:Codon; D003429:Cross Reactions; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D014453:Glucuronosyltransferase / Q000235:genetics* / Q000276:immunology; D006801:Humans; D008862:Microsomes, Liver / Q000201:enzymology*; D009693:Nucleic Acid Hybridization; D014176:Protein Biosynthesis; D012333:RNA, Messenger; D051381:Rats; D013045:Species Specificity
D000906:Antibodies; D003062:Codon; D012333:RNA, Messenger; D004247:DNA; D014453:Glucuronosyltransferase