Stability after freezing and thawing of solutions of mitomycin C in plastic minibags for intravesical use.
Solutions of 0.6 mg/ml mitomycin C (Mutamycin) in NaCl solution 0.9% in plastic minibags for intravesical use were stored at -20 degrees C and -30 degrees C and thawed by microwave radiation. Both unbuffered solutions and solutions buffered to approximately pH 7.5 were investigated. Concentrations were measured with HPLC. During freezing and storage at -20 degrees C sometimes crystallization of mitomycin C occurred. However, both buffered and unbuffered solutions could be frozen at -30 degrees C during at least four weeks and subsequently thawed without loss of content. Unbuffered mitomycin C solutions could be refrozen at -30 degrees C for another four weeks. Also buffered and unbuffered solutions of another brand of mitomycin C (Mitomycin-C Kyowa) could be frozen at -30 degrees C during four weeks without significant deterioration.
D000283:Administration, Intravesical; D002021:Buffers; D004349:Drug Packaging; D004355:Drug Stability; D005615:Freezing; D006863:Hydrogen-Ion Concentration; D016685:Mitomycin; D008937:Mitomycins / Q000008:administration & dosage; D012996:Solutions
D002021:Buffers; D008937:Mitomycins; D012996:Solutions; D016685:Mitomycin