[Effect of 17 alpha-ethinylestradiol on the activity of enzymes synthesizing and hydrolyzing cholesterol esters in the rat liver].
Intraperitoneal injection of 25 micrograms/100 g body weight of 17 alpha-ethinyl estradiol to rats was shown to decrease serum cholesterol and to increase hepatic cholesterol. The rise in the level of non-labeled and C14-labeled free and esterified cholesterol in hepatic homogenate, as well as in lysosomal and cytosol fractions was accompanied by reduced activity of acyl-CoA-cholesterol acyltransferase and increased activity of lysosomal cholesterol esterase, as compared with the controls. The activity of cytoplasmic cholesterol esterase remained practically unchanged. Fistula bile of treated rats collected during 30 min was analyzed for the concentration of free cholesterol and bile acids. It has been shown that treatment of rats with 17 alpha-ethinyl estradiol caused an increase in hepatic cholesterol elimination via bile pathways.
D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D001646:Bile / Q000378:metabolism; D002784:Cholesterol / Q000378:metabolism; D002788:Cholesterol Esters / Q000378:metabolism*; D004997:Ethinyl Estradiol / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D002787:Sterol Esterase / Q000378:metabolism; D002785:Sterol O-Acyltransferase / Q000378:metabolism; D001616:beta-Galactosidase / Q000378:metabolism
D002788:Cholesterol Esters; D004997:Ethinyl Estradiol; D002784:Cholesterol; D002785:Sterol O-Acyltransferase; D002787:Sterol Esterase; D000135:Acid Phosphatase; D001616:beta-Galactosidase
Vliianie 17 al'fa-étiniléstradiola na aktivnost' fermentov sinteza igidroliza éfirov kholesterina v pecheni krysy.