Gene transfer of immunoglobulin light chain restores heavy chain secretion.
Several lines of evidence suggest that immunoglobulin (Ig) light (L) chain plays a role in the secretion of heavy (H) chain. For example, myeloma variant lines, which synthesize the Ig H chain but not the L chain, fail to secrete H chain protein. Here we have tested directly the role of chain assembly in the control of Ig secretion by the transfer of functional L chain genes into two such L chain-defective myeloma mutants. A lambda 2 or kappa L chain gene was introduced into variant lines of the mouse myelomas MOPC 315 (IgA, lambda 2) or PC7 (IgM, kappa), respectively. Although the two mutant lines are unable to secrete the H chain they produce, rescue of secretion of complete Ig protein molecules (IgA or IgM) was observed after transfection. These results imply that the secretory apparatus of these cells is intact and that the failure to secrete free H chain reflects a structural feature intrinsic to that protein. The implications of these results with respect to control of secretion of multi-subunit proteins are discussed.
D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular; D004220:Disulfides; D007071:Immunoglobulin A, Secretory / Q000096:biosynthesis / Q000378:metabolism; D007143:Immunoglobulin Heavy Chains / Q000378:metabolism*; D007075:Immunoglobulin M / Q000096:biosynthesis / Q000378:metabolism; D007145:Immunoglobulin kappa-Chains / Q000235:genetics / Q000502:physiology; D007146:Immunoglobulin lambda-Chains / Q000235:genetics / Q000502:physiology; D046911:Macromolecular Substances; D051379:Mice; D014162:Transfection
D004220:Disulfides; D007071:Immunoglobulin A, Secretory; D007143:Immunoglobulin Heavy Chains; D007075:Immunoglobulin M; D007145:Immunoglobulin kappa-Chains; D007146:Immunoglobulin lambda-Chains; D046911:Macromolecular Substances