Induction of proliferation of B prolymphocytic leukemia cells by phorbol ester and native or recombinant interferon-gamma.
Phorbol ester phorbol myristate acetate (PMA) induces proliferation in nonmalignant human B cells and B cells from a patient with B prolymphocytic leukemia (B-PLL). Mitogen-free T cell-derived conditioned medium acts synergistically with PMA in inducing proliferation of B-PLL cells but does not enhance the PMA-stimulated outgrowth of nonmalignant B cells. Interleukin 2 (IL-2) has no effect on the outgrowth of B-PLL cells, and monoclonal antibodies against the IL-2 receptor do not influence the response to PMA and conditioned medium. Recombinant interferon-gamma (IFN-gamma), in contrast, is a potent enhancer of PMA-induced proliferation of B-PLL cells. With gel filtration techniques and with the use of anti-IFN-gamma antibodies, it is shown that IFN-gamma in the conditioned medium is responsible for the observed increase in B-PLL cell proliferation. Preincubation of B-PLL cells with IFN-gamma induces responsiveness to PMA, whereas IFN-gamma alone had no effect on these cells when pretreated with PMA. The combined data show that, in the presence of PMA, native and recombinant IFN-gamma are growth factors for B cells from a B-PLL patient and that IL-2 is not involved in this process.
D001402:B-Lymphocytes / Q000166:cytology / Q000473:pathology; D002455:Cell Division; D002460:Cell Line; D002850:Chromatography, Gel; D003470:Culture Media; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D007945:Leukemia, Lymphoid / Q000473:pathology*; D011994:Recombinant Proteins / Q000494:pharmacology*; D012016:Reference Values; D013601:T-Lymphocytes / Q000166:cytology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D003470:Culture Media; D011994:Recombinant Proteins; D007371:Interferon-gamma; D013755:Tetradecanoylphorbol Acetate