A simple radial immunodiffusion method for assay of beta 2-microglobulin in serum.
In this method for clinical measurement of beta 2-microglobulin, a 10 g/L agarose gel containing anti-beta 2-microglobulin is used, with subsequent staining with Coomassie Blue. Although the method is slow (requiring 30 h), it is inexpensive, reliable, and accurate over the range of 1 to 10 mg/L, and is useful in the determination of beta 2-microglobulin in serum and cerebrospinal fluid. A modified procedure in which staining with silver is used may be sufficiently sensitive for the clinical assay of urine.
D006801:Humans; D005779:Immunodiffusion / Q000379:methods; D007938:Leukemia / Q000378:metabolism; D008223:Lymphoma / Q000378:metabolism; D011863:Radioimmunoassay; D012016:Reference Values; D012394:Rosaniline Dyes; D013223:Statistics as Topic; D001613:beta 2-Microglobulin / Q000032:analysis* / Q000652:urine
D012394:Rosaniline Dyes; D001613:beta 2-Microglobulin; C004692:coomassie Brilliant Blue