Transplantation of fetal fibroblasts and correction of enzymatic deficiencies in patients with Hunter's or Hurler's disorders.
An attempt was made at correcting the specific lysosomal enzyme deficiencies in 7 children with Hunter's or Hurler's diseases by transplantation of fetal fibroblasts. In spite of pretreating the young patients with stored blood, following a procedure employed successfully to avoid rejection of kidneys from incompatible donors, the use of serum-free media for culturing the cells before being harvested and incubation of the cells with chorionic gonadotrophin, the transplantation of fetal fibroblasts was not associated with biochemical or clinical changes. None of the seven patients showed immune reactions against the transplanted cells, HLA antigens, or the missing enzymes.
D000818:Animals; D002417:Cattle / Q000097:blood; D002478:Cells, Cultured; D003470:Culture Media / Q000276:immunology; D005333:Fetus / Q000166:cytology; D005347:Fibroblasts / Q000201:enzymology / Q000276:immunology / Q000637:transplantation*; D006025:Glycosaminoglycans / Q000652:urine; D006648:Histocompatibility; D006801:Humans; D007068:Iduronidase / Q000172:deficiency; D008247:Lysosomes / Q000201:enzymology; D008059:Mucopolysaccharidosis I / Q000276:immunology / Q000628:therapy* / Q000652:urine; D016532:Mucopolysaccharidosis II / Q000276:immunology / Q000628:therapy* / Q000652:urine; D009844:Oligosaccharides / Q000652:urine
D003470:Culture Media; D006025:Glycosaminoglycans; D009844:Oligosaccharides; D007068:Iduronidase