Bursal role in the synthesis of gamma-chain messenger ribonucleic acid in young chickens.
Incomplete bursectomy was performed in 3-day-old chickens by treatment with .5% testosterone propionate on the 3rd day of incubation. The purpose of this experimental approach was to determine the role of the bursa of Fabricius in the translation activity of messenger ribonucleic acids (RNA) coding immunoglobulin-isotypes. The bursal poly(A) RNA, prepared from bursae of normal and hormone-treated chickens, directed the synthesis of 72, 43, 38, and 25-kilodalton molecules as revealed by immune precipitation and sodium dodecylsulphate acrylamide gel electrophoresis. However, unlike the messenger RNA from normal bursae, the messenger from bursae of hormonal-treated embryos failed to direct the synthesis of 68-kilodalton gamma-polypeptide.
D000818:Animals; D002060:Bursa of Fabricius / Q000276:immunology*; D002645:Chickens; D004591:Electrophoresis, Polyacrylamide Gel; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D007142:Immunoglobulin gamma-Chains / Q000235:genetics*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics* / Q000302:isolation & purification
D007143:Immunoglobulin Heavy Chains; D007142:Immunoglobulin gamma-Chains; D012333:RNA, Messenger