In-111 Pseudomonas aeruginosa: a simple method of labeling live bacteria with a gamma-emitting radioisotope.
We describe a simple and reliable technique for labeling Pseudomonas aeruginosa with a readily available commercial preparation of indium-111 (111In) oxine. Labeling of a heavy bacterial suspension with 500 mu Ci of commercially prepared 111In-oxine resulted in a yield of 0.0004 mu Ci of cell-associated 111In per 10(6) colony-forming units (CFU). The label was 88% bacterially associated and did not effect viability of the organism. Radiolabeling a gram-negative organism with 111In-oxine provides a non-toxic, stable gamma-emitting bacterial tracer.
D000818:Animals; D001769:Blood / Q000382:microbiology; D003470:Culture Media; D004285:Dogs; D006912:Hydroxyquinolines; D007204:Indium; D009942:Organometallic Compounds; D015125:Oxyquinoline / Q000031:analogs & derivatives; D011550:Pseudomonas aeruginosa / Q000302:isolation & purification / Q000502:physiology; D011868:Radioisotopes; D013234:Stem Cells; D013997:Time Factors
D003470:Culture Media; D006912:Hydroxyquinolines; D009942:Organometallic Compounds; D011868:Radioisotopes; D007204:Indium; C032950:indium oxine; D015125:Oxyquinoline