A simple microassay for interleukin-2 activity.
A simple in vitro assay is described to quantitate human interleukin-2 (IL-2). It is based on the spontaneous' activation of human peripheral T cells in tissue culture and the subsequent expression of the IL-2 receptor. The results obtained with this assay agree with those of assays which utilise IL-2 dependent T cell clones. The method is highly sensitive, reproducible and easy to perform.
D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface / Q000032:analysis; D002478:Cells, Cultured; D006133:Growth Substances / Q000494:pharmacology; D006801:Humans; D007376:Interleukin-2 / Q000032:analysis*; D008213:Lymphocyte Activation / Q000187:drug effects; D008832:Microchemistry; D009000:Monocytes / Q000276:immunology; D010641:Phenotype; D010835:Phytohemagglutinins / Q000494:pharmacology; D011971:Receptors, Immunologic / Q000032:analysis*; D015375:Receptors, Interleukin-2; D011994:Recombinant Proteins / Q000494:pharmacology; D013601:T-Lymphocytes / Q000032:analysis*
D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface; D006133:Growth Substances; D007376:Interleukin-2; D010835:Phytohemagglutinins; D011971:Receptors, Immunologic; D015375:Receptors, Interleukin-2; D011994:Recombinant Proteins