Purification to homogeneity of a human hematopoietic growth factor that stimulates the growth of a murine interleukin 3-dependent cell line.
A human lymphokine derived from the 5637 bladder carcinoma has been purified to homogeneity by using sequential reverse-phase high pressure liquid chromatography. A high recovery of biological activity is obtained by using this purification. The NH2-terminal amino acid sequence shows no homology to human interleukin 1 (IL 1), human IL 2, murine IL 3, or human granulocyte-macrophage colony-stimulating factor. The growth-promoting properties of the 5637-derived factor can be rapidly assayed by using the murine IL 3-dependent 32D c1-23 cell line. The amino acid sequence described is identical to that recently described for a human granulocyte colony-stimulating factor.
D000818:Animals; D001854:Bone Marrow Cells; D002460:Cell Line; D003114:Colony-Forming Units Assay; D003115:Colony-Stimulating Factors / Q000502:physiology*; D004591:Electrophoresis, Polyacrylamide Gel; D005590:Fractional Precipitation; D006133:Growth Substances / Q000302:isolation & purification* / Q000502:physiology; D016298:Hematopoietic Cell Growth Factors; D006801:Humans; D007377:Interleukin-3; D008222:Lymphokines / Q000502:physiology*; D051379:Mice; D008970:Molecular Weight
D003115:Colony-Stimulating Factors; D006133:Growth Substances; D016298:Hematopoietic Cell Growth Factors; D007377:Interleukin-3; D008222:Lymphokines