Spectrophotometric method for the assay of human blood coagulation factor VIII.
A spectrophotometric method for the assay of human blood coagulation factor VIII in plasma is presented. The chromogenic assay for factor VIII:C in plasma is performed in 3 steps: activation of factor VIII by thrombin; activation of factor X in a mixture of factor X, factor IXa, phospholipids/Ca2+ and plasma containing activated factor VIII, and determination of the rate of factor Xa formation with the chromogenic substrate S2337. Within-assay variation was between 5 and 6.9% for factor VIII:C activities between 20 and 150%. Clotting and chromogenic factor VIII:C activities were compared in plasma of 50 normal healthy donors (coefficient of correlation r = 0.83).
D001780:Blood Coagulation Tests; D005169:Factor VIII / Q000378:metabolism*; D006801:Humans; D012016:Reference Values; D013053:Spectrophotometry / Q000295:instrumentation / Q000379:methods*
D005169:Factor VIII