title: Mechanism of counteraction of aflatoxin toxicity by thymine and folic acid in Bacillus megaterium.
abstract: Thymine and cytosine as well as the intermediates in pyrimidine biosynthetic pathway dihydroorotic acid, orotic acid, carbamyl aspartate and 5'-uridine monophosphate and folic acid, was synergistic. 2-14C-Thymine, 6-14C-orotic acid and 14C-formate but not 2-14C-uracil, were incorporated into DNA more in the presence of aflatoxin. These findings indicated that aflatoxin inhibited the pyrimidine base synthesis which could be overcome to a great extent by the addition of thymine and folic acid to aflatoxin-treated cells.
mesh_terms: D016604:Aflatoxin B1; D000348:Aflatoxins / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D000630:Aminopterin / Q000494:pharmacology; D001224:Aspartic Acid / Q000494:pharmacology; D001410:Bacillus megaterium / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D004269:DNA, Bacterial / Q000378:metabolism; D004357:Drug Synergism; D005492:Folic Acid / Q000378:metabolism / Q000494:pharmacology*; D009963:Orotic Acid / Q000378:metabolism / Q000494:pharmacology; D013941:Thymine / Q000378:metabolism / Q000494:pharmacology*; D014498:Uracil / Q000378:metabolism / Q000494:pharmacology; D014542:Uridine Monophosphate / Q000494:pharmacology
chemical_list: D000348:Aflatoxins; D004269:DNA, Bacterial; D001224:Aspartic Acid; D014498:Uracil; D009963:Orotic Acid; D005492:Folic Acid; D016604:Aflatoxin B1; D014542:Uridine Monophosphate; D000630:Aminopterin; D013941:Thymine