Altered cellular response to UV irradiation in a patient affected by premature ageing.
An abnormal response to UV-irradiation was found in a patient affected by precocious senescence. A decreased level of unscheduled DNA synthesis (UDS) was present in 60% of Go lymphocytes and in fibroblasts after the fifth culture passage. Hypersensitivity of lymphocytes to UV-light was indicated also by a decreased rate of DNA synthesis after mitogen stimulation. The results of this study indicate that the defect which determines the premature ageing influences the capacity to repair UV-induced DNA damage.
D000328:Adult; D004260:DNA Repair / Q000187:drug effects / Q000528:radiation effects*; D004261:DNA Replication / Q000187:drug effects / Q000528:radiation effects; D005020:Ethyl Methanesulfonate / Q000494:pharmacology; D005347:Fibroblasts / Q000187:drug effects / Q000528:radiation effects; D006801:Humans; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000187:drug effects / Q000528:radiation effects; D008297:Male; D008875:Middle Aged; D016685:Mitomycin; D008937:Mitomycins / Q000494:pharmacology; D011371:Progeria / Q000235:genetics*; D014466:Ultraviolet Rays
D008937:Mitomycins; D016685:Mitomycin; D005020:Ethyl Methanesulfonate