title: One step purification of Escherichia coli beta-glucuronidase.
abstract: beta-glucuronidase was purified by affinity chromatography on thiophenyl-glucuronide coupled to Sepharose. The enzyme was more than 95% pure. This enzyme is a tetramer composed of identical 74 kDa monomers. The amino-terminal sequence determined was: NH2-Met-Leu-Arg-Pro-Val.
mesh_terms: D000595:Amino Acid Sequence; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology*; D005966:Glucuronidase / Q000302:isolation & purification*; D008970:Molecular Weight
chemical_list: D005966:Glucuronidase