[Role of acetate and butyrate in the induction of NADH: rubredoxin oxidoreductase in Clostridium acetobutylicum].
Study of the biosynthesis of NADH: rubredoxin oxidoreductase in resting cells of Clostridium acetobutylicum shows that this enzyme is synthesized at a maximal rate in the presence of acetic acid at a concentration of 3 g . l-1 and at pH 4.8. Protons do not play any role in this biosynthesis since no induction is observed in a medium without acetate for the same values of pH. Butyric acid at a concentration of 0.5 g . l-1 gives 50% induction and formic acid, isobutyric acid and propionic acid have no inductive action on NADH: rubredoxin oxidoreductase. These results are confirmed by studies using a dialysis bag. Only a culture against acetic acid at an initial concentration of 2 g . l-1 gives maximal biosynthesis of the enzyme, whereas a culture in which all products of metabolism are eliminated gives an activity which is 80% lower.
D000085:Acetates / Q000494:pharmacology*; D019342:Acetic Acid; D002087:Butyrates / Q000494:pharmacology*; D020148:Butyric Acid; D003013:Clostridium / Q000201:enzymology*; D003956:Dialysis; D004790:Enzyme Induction / Q000187:drug effects; D005561:Formates / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D058610:Isobutyrates; D007700:Kinetics; D009247:NADH, NADPH Oxidoreductases / Q000096:biosynthesis*; D011422:Propionates / Q000494:pharmacology
D000085:Acetates; D002087:Butyrates; D005561:Formates; D058610:Isobutyrates; D011422:Propionates; C030544:formic acid; D020148:Butyric Acid; C020380:isobutyric acid; C023091:rubredoxin-NAD+ reductase; D009247:NADH, NADPH Oxidoreductases; C029658:propionic acid; D019342:Acetic Acid
Rôle de l'acétate et du butyrate dans l'induction de la NADH: rubrédoxine oxydoréductase chez Clostridium acetobutylicum.