title: Detection of TRH extended peptides in rat hypothalamus using an antibody raised to pGluHisProGlyLys.
abstract: An antibody was raised to the synthetic pentapeptide pGluHisProGlyLys which, in radioimmunoassay (RIA), could detect the pentapeptide at a level of 10 fmole per tube and exhibited less than 0.5 per cent cross reactivity with a series of related peptides. The RIA was used to demonstrate the presence of C-terminally extended forms of thyrotropin releasing hormone (TRH) in rat hypothalamus. After extraction, the endogenous peptides were resolved by gel exclusion chromatography and TRH-extended peptides were revealed by trypsin digestion to release the pentapeptide. The TRH extended peptides occurred in substantial quantity, approximately 11 pmoles/g, indicating that only partial processing of the gene duplicated prohormone takes place.
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology; D000918:Antibody Specificity; D005260:Female; D007031:Hypothalamus / Q000032:analysis*; D009842:Oligopeptides / Q000276:immunology*; D010446:Peptide Fragments / Q000032:analysis* / Q000276:immunology; D011498:Protein Precursors / Q000032:analysis*; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D013973:Thyrotropin-Releasing Hormone / Q000032:analysis* / Q000276:immunology
chemical_list: D000906:Antibodies; D009842:Oligopeptides; D010446:Peptide Fragments; D011498:Protein Precursors; D013973:Thyrotropin-Releasing Hormone