Mechanism of counteraction of aflatoxin toxicity by thymine and folic acid in Bacillus megaterium.
Thymine and cytosine as well as the intermediates in pyrimidine biosynthetic pathway dihydroorotic acid, orotic acid, carbamyl aspartate and 5'-uridine monophosphate and folic acid, was synergistic. 2-14C-Thymine, 6-14C-orotic acid and 14C-formate but not 2-14C-uracil, were incorporated into DNA more in the presence of aflatoxin. These findings indicated that aflatoxin inhibited the pyrimidine base synthesis which could be overcome to a great extent by the addition of thymine and folic acid to aflatoxin-treated cells.
D016604:Aflatoxin B1; D000348:Aflatoxins / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D000630:Aminopterin / Q000494:pharmacology; D001224:Aspartic Acid / Q000494:pharmacology; D001410:Bacillus megaterium / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D004269:DNA, Bacterial / Q000378:metabolism; D004357:Drug Synergism; D005492:Folic Acid / Q000378:metabolism / Q000494:pharmacology*; D009963:Orotic Acid / Q000378:metabolism / Q000494:pharmacology; D013941:Thymine / Q000378:metabolism / Q000494:pharmacology*; D014498:Uracil / Q000378:metabolism / Q000494:pharmacology; D014542:Uridine Monophosphate / Q000494:pharmacology
D000348:Aflatoxins; D004269:DNA, Bacterial; D001224:Aspartic Acid; D014498:Uracil; D009963:Orotic Acid; D005492:Folic Acid; D016604:Aflatoxin B1; D014542:Uridine Monophosphate; D000630:Aminopterin; D013941:Thymine