Two distinct events in lineage establishment of a biopotential hemopoietic cell line.
HL-60, a human myelomonocytic cell line can be induced to differentiate along either granulocyte or monocyte pathways and therefore is a good model to study lineage establishment. We have shown that there are two classes of reactions in the establishment of the granulocytic line distinguishable by their kinetics. The fast reaction occurs in hours and is induced by ouabain or A23187, agents which effect ions. The slow reactions occur at a constant rate over a period of 5 days and are induced by the surrogate inducers RA and D3 but also by the natural inducer GM-CSF. GM-CSF induces the enzyme markers which we use to characterize both granulocytes and monocytes in the same cell. If these markers are indeed unique to each of the lines this means that HL-60 has both gene programs initiated by GM-CSF and D3. Variant lines which do not respond to one or more of the inducers can be used to analyze this point since variants which are induced only to granulocytes or only to monocytes by GM-CSF have been isolated.
D000001:Calcimycin / Q000494:pharmacology; D002117:Calcitriol / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D003115:Colony-Stimulating Factors / Q000494:pharmacology; D014644:Genetic Variation; D006098:Granulocytes / Q000166:cytology; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D007700:Kinetics; D009000:Monocytes / Q000166:cytology; D010042:Ouabain / Q000494:pharmacology; D014212:Tretinoin / Q000494:pharmacology
D003115:Colony-Stimulating Factors; D000001:Calcimycin; D014212:Tretinoin; D010042:Ouabain; D002117:Calcitriol