title: Molecular differentiation of neurons from ependyma-derived cells in tissue cultures of regenerating teleost spinal cord.
abstract: Cells cultured from the caudalmost area of regenerating teleost spinal cord differ both morphologically and in terms of molecular architecture from those of more rostral (more fully differentiated) areas of the cord. The caudalmost regenerating cord consists of an ependymal tube. Cells from this region have flattened or partially flattened cell somas and short spike projections in vitro; they do not exhibit the rounded cell somas nor the long, thin, branching neurites typical of differentiated neurons. A series of cultures taken from different areas along the length of regenerating spinal cord were examined for molecular differentiation by staining with a monoclonal antibody against non-phosphorylated neurofilament protein (SMI 32). None of the cells from the caudalmost culture of the regenerating spinal cord stained with antibody SMI 32. In cultures of more rostral regenerated cord, the cells with neuronal morphology do stain positively with the anti-neurofilament antibody. This result suggests that the cells in the cultures of caudalmost cord represent relatively undifferentiated ependymal cells, or ependyma-derived cells, which later differentiate into neurons and glia in the regenerating spinal cord.
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D002454:Cell Differentiation; D002478:Cells, Cultured; D004555:Electric Fish / Q000502:physiology*; D004805:Ependyma / Q000032:analysis / Q000166:cytology* / Q000502:physiology; D007381:Intermediate Filament Proteins / Q000032:analysis; D009416:Nerve Regeneration; D016900:Neurofilament Proteins; D009474:Neurons / Q000032:analysis / Q000502:physiology; D013116:Spinal Cord / Q000032:analysis / Q000166:cytology* / Q000502:physiology
chemical_list: D000911:Antibodies, Monoclonal; D007381:Intermediate Filament Proteins; D016900:Neurofilament Proteins