title: Characterization of chromosome and plasmid transformation in Bacillus subtilis using gently lysed protoplasts.
abstract: Competent cells of Bacillus subtilis were transformed with DNA from gently lysed protoplasts. Significant linkages among markers separated by distances of approximately 2.3% of the total chromosome were found, which have not been detected for conventional transformation. In comparison to previous reports, enhanced plasmid transformation was observed [4.0 X 10(7) transformants per microgram DNA (one transformant per 5 X 10(4) molecules added)], when competent cells were transformed with DNA from lysed protoplasts harboring pUB110.
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics*; D002876:Chromosomes, Bacterial; D004269:DNA, Bacterial / Q000235:genetics; D008040:Genetic Linkage; D010957:Plasmids; D011523:Protoplasts; D014169:Transformation, Bacterial
chemical_list: D004269:DNA, Bacterial