Trial of isolation of a virus from sera of patients with Kawasaki disease.
We tried to isolate pathogenic viruses from specimens of patients with Kawasaki disease. Blood clots and sera, spinal fluid, throat swabs, stool and urine from 24 patients with Kawasaki disease were studied. The specimens were inoculated into HEL cells and Vero cells. The cells were observed for one month, but no cytopathic effect (CPE) occurred. Blind passage was performed, but no degeneration of the second series of cells was observed. Fluorescent antibody indirect methods to determine viral antigens in cells inoculated with patients' specimens was also negative.
D002675:Child, Preschool; D006801:Humans; D007223:Infant; D009080:Mucocutaneous Lymph Node Syndrome / Q000382:microbiology*; D014766:Viremia / Q000382:microbiology*; D014780:Viruses / Q000302:isolation & purification*