[Casein-like immunoreactivity in the male rat].
A substance presenting a beta-casein-like immunoreactivity (beta-cas-LI) was found in male serum and tissues. This beta-cas-LI was measured by a specific radioimmunoassay using a purified beta-casein and an anti beta-casein kindly supplied by L. M. Houdebine. Serial dilutions of serum or tissues samples showed a good parallelism to the standard curve. In male rat, beta-cas-Li was found in all sera and in some tissues = mammary gland, prostate and testis which contained respectively 6,200, 408 and 38 ng per organ. beta-cas-LI value in the serum was regulatable. Hypophysectomy and long-term castration strongly reduced basal levels. Insertion of testosterone silastic capsules (25 mg/capsule) was able to restore control values, while estradiol (E2) (4 mg/kg E2 valerianate) to castrated or intact rats increased serum beta-cas-LI above basal values. In conclusion, these results showed, for the first time, the presence in male rat serum and tissues of a beta-casein immunoreactive-like substance. Moreover, this beta-cas-LI seemed to be under pituitary and gonad regulation.
D000818:Animals; D002364:Caseins / Q000032:analysis* / Q000097:blood; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D007774:Lactation; D008297:Male; D008321:Mammary Glands, Animal / Q000032:analysis; D011247:Pregnancy; D011467:Prostate / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D013737:Testis / Q000032:analysis; D014018:Tissue Distribution
D002364:Caseins; D004958:Estradiol
Immunoactivité semblable à la caséine chez le rat mâle.