title: The influence of glutathione and other thiols on human platelet aggregation.
abstract: The platelet membrane contains sulfhydryl groups which are essential for normal platelet function. Reduced glutathione (GSH) and other thiols such as cysteine and 6-mercaptopurine were found to inhibit human platelet aggregation induced by adenosine diphosphate (ADP), collagen and arachidonic acid. The inhibition of ADP-induced aggregation by GSH (IC50 = 0.61 +/- 0.05 mM) was greater than that by cysteine (IC50 = 13 +/- 1 mM) or 6-mercaptopurine (IC50 = 5.4 +/- 0.2 mM). Two other thiols, dithiothreitol and beta-mercaptoethanol were found to cause platelet aggregation instead of inhibition. The interaction of GSH with the ADP receptor was noncompetitive in nature.
mesh_terms: D000244:Adenosine Diphosphate / Q000494:pharmacology; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000494:pharmacology; D001792:Blood Platelets / Q000166:cytology / Q000187:drug effects; D002462:Cell Membrane / Q000187:drug effects; D003094:Collagen / Q000494:pharmacology; D003545:Cysteine / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005978:Glutathione / Q000494:pharmacology*; D006801:Humans; D015122:Mercaptopurine / Q000494:pharmacology; D010974:Platelet Aggregation / Q000187:drug effects*; D013438:Sulfhydryl Compounds / Q000494:pharmacology*
chemical_list: D001095:Arachidonic Acids; D013438:Sulfhydryl Compounds; D016718:Arachidonic Acid; D000244:Adenosine Diphosphate; D003094:Collagen; D015122:Mercaptopurine; D005978:Glutathione; D003545:Cysteine