Dissociation between the expression of IL 2 receptor and IL 2 receptor mRNA in the antigen-specific T cell clone stimulated by the specific antigen with B cell APC.
An antigen-presenting capacity of B cells was analyzed in comparison with that of whole spleen cells in terms of IL 2R expression of a human IgG-specific T cell clone, 24-2C.3. When the clone was stimulated with the antigen and irradiated spleen cells, the clone expressed an adequate amount of IL 2R on the surface in flow cytometric analysis. On the other hand, the clone expressed the receptor only poorly, being stimulated with B cell APC. The findings were confirmed by Western blot analysis on the clone solubilized by Nonidet P-40 and also by fluorescent antibody technique on the fixed clone. 24-2C.3 clone stimulated with B cell APC was also suggested not to secrete IL 2R in culture medium. In dot blot hybridization analysis performed with cDNA probe, however, 24-2C.3 clone stimulated with B cell APC was shown to express a comparable amount of IL 2R mRNA to the clone stimulated with whole spleen cells as APC. The mRNA expressed in the clones stimulated with B cells and with spleen cells could not be distinguished from each other in Northern blot analysis. The culture supernatant of 24-2C.3 clone incubated with the antigen and irradiated spleen cells was shown to enhance the expression of IL 2R on the clone stimulated with B cell APC. These results suggest that there is some defect in the antigen-presentation capacity of B cells in terms of the elicitation of the intracellular signal for IL 2R expression in T cells and that the humoral factor(s) produced in the presence of splenic adherent cells could supply the defect.
D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology*; D000941:Antigens; D001402:B-Lymphocytes / Q000276:immunology*; D002453:Cell Cycle; D002999:Clone Cells; D005786:Gene Expression Regulation; D007074:Immunoglobulin G / Q000276:immunology; D007376:Interleukin-2 / Q000502:physiology*; D008222:Lymphokines / Q000502:physiology; D051379:Mice; D012333:RNA, Messenger / Q000235:genetics; D011971:Receptors, Immunologic / Q000502:physiology*; D015375:Receptors, Interleukin-2; D013601:T-Lymphocytes / Q000502:physiology*
D000941:Antigens; D007074:Immunoglobulin G; D007376:Interleukin-2; D008222:Lymphokines; D012333:RNA, Messenger; D011971:Receptors, Immunologic; D015375:Receptors, Interleukin-2