Effects of methylprednisolone and the combination of alpha-tocopherol and selenium on arachidonic acid metabolism and lipid peroxidation in traumatized spinal cord tissue.
Traumatic injury of the spinal cord leads to a series of pathological events that result in tissue necrosis and paralysis. Among the earliest biochemical reactions are hydrolysis of fatty acids from membrane phospholipids, production of biologically active eicosanoids, and peroxidation of lipids. This study examines the effect of agents purported to improve recovery following spinal cord trauma, methylprednisolone sodium succinate (MPSS) and the combination of alpha-tocopherol and selenium (Se), on the posttraumatic alterations of membrane lipid metabolism. Pretreatment with either MPSS or alpha-tocopherol and Se reduced the trauma-induced release of total FFA including arachidonate in the injured spinal cord tissue. In addition, these agents decreased the postinjury levels of prostanoids. Pretreatment with either MPSS or alpha-tocopherol and Se also completely prevented the trauma-induced loss of cholesterol while inhibiting the increase of a cholesterol peroxidation product, 25-hydroxycholesterol. These data suggest that: perturbation of membrane lipid metabolism may contribute to the tissue necrosis and functional deficit of spinal cord injury and MPSS or the combination of alpha-tocopherol and Se may protect injured spinal cord tissue, at least in part, by limiting these posttraumatic membrane lipid changes.
D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism*; D002415:Cats; D002784:Cholesterol / Q000032:analysis; D005230:Fatty Acids, Nonesterified / Q000378:metabolism; D008054:Lipid Peroxides / Q000378:metabolism*; D008775:Methylprednisolone / Q000494:pharmacology*; D010743:Phospholipids / Q000032:analysis; D011817:Rabbits; D013116:Spinal Cord / Q000032:analysis / Q000187:drug effects / Q000378:metabolism; D013117:Spinal Cord Compression / Q000378:metabolism; D013119:Spinal Cord Injuries / Q000378:metabolism*; D014810:Vitamin E / Q000494:pharmacology*
D001095:Arachidonic Acids; D005230:Fatty Acids, Nonesterified; D008054:Lipid Peroxides; D010743:Phospholipids; D014810:Vitamin E; D016718:Arachidonic Acid; D002784:Cholesterol; D008775:Methylprednisolone