Control of prostaglandin E2 synthesis in cultured rat inner medullary collecting tubule cells: the role of calcium.
We investigated the role of calcium in basal, ionophore- and hormone-stimulated prostaglandin E2 (PGE2) production by rat inner medullary collecting tubule cells. Basal PGE2 production is significantly decreased in the absence of calcium (4.49 +/- 0.79 vs. 19.99 +/- 3.47 pg/micrograms protein/h, p less than 0.001). No further increment is seen at 4 mM calcium. The ability of the calcium ionophore A23187 to stimulate PG production is proportional to the amount of calcium present in the extracellular space. Bradykinin is a potent stimulus to PG production even in the virtual absence of calcium while arginine vasopressin has only a modest effect. Neither bradykinin nor arginine vasopressin exhibit any further PG stimulation at 1 mM calcium; both bradykinin and arginine vasopressin increase PGE2 production significantly when calcium is increased to a supraphysiologic level. The data suggests an important role for intracellular rather than extracellular calcium in the control of PG production. Bradykinin but not vasopressin display significant calcium-independent stimulatory effects on PG synthesis.
D000818:Animals; D001127:Arginine Vasopressin / Q000494:pharmacology; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D015232:Dinoprostone; D007679:Kidney Medulla / Q000378:metabolism*; D007684:Kidney Tubules / Q000378:metabolism*; D007685:Kidney Tubules, Collecting / Q000378:metabolism*; D008297:Male; D011458:Prostaglandins E / Q000096:biosynthesis*; D051381:Rats; D011919:Rats, Inbred Strains
D011458:Prostaglandins E; D001127:Arginine Vasopressin; D000001:Calcimycin; D015232:Dinoprostone; D002118:Calcium