Transplantation of putative preneoplastic hepatocytes of analbuminemic rats in the livers of Sprague-Dawley x analbuminemic hybrid rats.
As the analbuminemia of analbuminemic rats (NAR) is inherited as a recessive trait, the livers of F1 hybrids of Sprague-Dawley X NAR (SD X NAR) retain the capability to produce albumin. We induced albumin-negative hyperplastic nodules in the livers of NAR, isolated the nodule cells and transplanted them in the livers of SD X NAR by infusion into the mesenteric vein. When the hosts were treated with dietary 2-acetylaminofluorene plus a partial hepatectomy, numerous albumin(-) nodules of donor origin were formed within the albumin(+) livers of the hosts. This system should be useful to analyze sequential events during hepatocarcinogenesis by using a genotypic marker.
D000818:Animals; D005982:Glutathione Transferase / Q000032:analysis; D006824:Hybridization, Genetic; D008114:Liver Neoplasms, Experimental / Q000473:pathology*; D008297:Male; D009368:Neoplasm Transplantation; D011230:Precancerous Conditions / Q000473:pathology*; D051381:Rats; D011919:Rats, Inbred Strains; D012709:Serum Albumin / Q000172:deficiency*
D012709:Serum Albumin; D005982:Glutathione Transferase