Production of specific antibodies against protein A fusion proteins.
The gene for Staphylococcal protein A was fused to the coding sequence of bacterial beta-galactosidase, alkaline phosphatase and human insulin-like growth factor I (IGF-I). The fusion proteins, expressed in bacteria, were purified by affinity chromatography on IgG-Sepharose and antibodies were raised in rabbits. All three fusion proteins elicited specific antibodies against both the inserted protein sequences and the protein A moiety. In the case of IGF-I, the protein A moiety in the fusion protein may act as an adjuvant since native IGF-I alone is a poor immunogen. The results suggest that the protein A fusion system can be used for efficient antibody production against peptides or proteins expressed from cloned or synthetic genes. To facilitate such gene fusions a set of optimized vectors have been constructed.
D000469:Alkaline Phosphatase / Q000032:analysis; D000595:Amino Acid Sequence; D000906:Antibodies; D000936:Antigen-Antibody Complex; D001483:Base Sequence; D004926:Escherichia coli / Q000235:genetics; D005796:Genes; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000032:analysis; D011993:Recombinant Fusion Proteins / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D011994:Recombinant Proteins / Q000032:analysis*; D013205:Staphylococcal Protein A / Q000032:analysis* / Q000276:immunology; D001616:beta-Galactosidase / Q000032:analysis
D000906:Antibodies; D000936:Antigen-Antibody Complex; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D013205:Staphylococcal Protein A; D007334:Insulin-Like Growth Factor I; D000469:Alkaline Phosphatase; D001616:beta-Galactosidase