A method for the mass isolation of islets from the adult pig pancreas.
A method for mass isolation of islets from the pig pancreas is described. The procedure involves the use of an enzymatic and mechanical pancreatic digestion procedure followed by filtration and separation of the islets on Ficoll gradients. A remarkably high yield of purified islets has been obtained from the pig pancreas with this procedure. The islets are morphologically intact and respond to acute stimulation with glucose in vitro.
D000818:Animals; D005947:Glucose / Q000494:pharmacology; D007328:Insulin / Q000032:analysis; D007515:Islets of Langerhans / Q000032:analysis / Q000166:cytology; D016381:Islets of Langerhans Transplantation; D008722:Methods; D051379:Mice; D008810:Mice, Inbred C57BL; D009924:Organ Culture Techniques; D013552:Swine; D014183:Transplantation, Heterologous
D007328:Insulin; D005947:Glucose