Effects of total parenteral nutrition on the development of intestinal endotoxemia in rats: a comparison between glucose and lipid.
The effects of parenteral nutrition (PN) and of the difference in the PN regimens between glucose and lipid emulsion on the development of endogenous endotoxemia were studied in 40 Wister rats. Endotoxemia was induced by occluding the superior mesenteric vein (SMV) for 30 min. The plasma endotoxin in the portal blood at the time of the release of the SMV occlusion and that in the arterial blood 10 min after the release were quantified. Twenty of the 40 rats had received PN for 48 hr prior to the SMV occlusion. Ten of these 20 rats received the total nonprotein calorie (TNPC) solely with glucose, and the other 10 rats received 25% of the TNPC with lipid emulsion. Ten rats had been allowed free access to lab food until the SMV occlusion. The remaining 10 rats underwent neither the SMV occlusion nor PN, and served as the control group. Both the portal and the arterial endotoxin increased after the release of the SMV occlusion, however the portal endotoxin was higher than that of the arterial one. Both the portal and the arterial endotoxin of the rats supported by PN were significantly lower than those of the rats nourished by lab food, while they were higher than the control values. The difference in the PN regimens did not cause any alteration in the endotoxin levels. These results indicate that the development of intestinal endotoxemia was not influenced by the difference in the PN regimens, but it was rather influenced by a presence of intestinal content.
D000818:Animals; D004731:Endotoxins / Q000096:biosynthesis / Q000097:blood*; D005217:Fat Emulsions, Intravenous / Q000494:pharmacology*; D005947:Glucose / Q000494:pharmacology*; D007410:Intestinal Diseases / Q000517:prevention & control*; D008297:Male; D008642:Mesenteric Veins; D010289:Parenteral Nutrition, Total; D010587:Phagocytosis / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains
D004731:Endotoxins; D005217:Fat Emulsions, Intravenous; D005947:Glucose