title: Prostaglandin involvement in the evoked release of D-aspartate from cerebellar mossy fiber terminals.
abstract: Isolated cerebellar glomeruli, containing mossy fiber terminals, were used to investigate the mechanisms involved in the evoked release of acid amino acids. The glomeruli contain a high affinity uptake system for D-aspartate, with a KT of 384 pmol/mg protein/min and the incorporated D-[3H]aspartate was released in response to various depolarizing agents, as well as exogenous arachidonic acid and prostaglandins. There was a marked inhibition of the release evoked by high K+ and exogenous arachidonate when the cyclooxygenase inhibitor ibuprofen was present. Also, exposure of the glomeruli to depolarizing conditions resulted in an increase in the amount of unesterified [3H]arachidonate. It appears that accumulation of unesterified arachidonate and subsequent production of prostaglandins are involved in the evoked release of the acidic amino acids from cerebellar mossy fiber terminals.
mesh_terms: D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism; D001224:Aspartic Acid / Q000378:metabolism*; D001692:Biological Transport; D002531:Cerebellum / Q000187:drug effects / Q000378:metabolism*; D015237:Dinoprost; D015232:Dinoprostone; D007052:Ibuprofen / Q000494:pharmacology; D007213:Indomethacin / Q000494:pharmacology; D007700:Kinetics; D011188:Potassium / Q000494:pharmacology; D011453:Prostaglandins / Q000494:pharmacology* / Q000502:physiology; D011458:Prostaglandins E / Q000494:pharmacology; D011460:Prostaglandins F / Q000494:pharmacology; D051381:Rats; D013237:Stereoisomerism; D013779:Tetrodotoxin / Q000494:pharmacology; D014700:Verapamil / Q000494:pharmacology
chemical_list: D001095:Arachidonic Acids; D011453:Prostaglandins; D011458:Prostaglandins E; D011460:Prostaglandins F; D016718:Arachidonic Acid; D001224:Aspartic Acid; D013779:Tetrodotoxin; D015237:Dinoprost; D014700:Verapamil; D015232:Dinoprostone; D011188:Potassium; D007052:Ibuprofen; D007213:Indomethacin