High-affinity uptake of taurine and beta-alanine in primary cultures of rat astrocytes.
The kinetics and specificity of taurine and beta-alanine uptake were studied in primary cultures of rat astrocytes under identical experimental conditions. The uptake consisted of nonsaturable penetration and saturable high-affinity transport that was strictly sodium dependent. The cells accumulated taurine more effectively than beta-alanine, both the affinity and uptake capacity being greater for taurine. Taurine uptake was competitively inhibited by beta-alanine and GABA, the former being more potent. Also, hypotaurine and 2-guanidinoethanesulphonic acid strongly reduced taurine uptake, but L-2,4-diaminobutyric acid had no significant effect. beta-Alanine uptake was also competitively inhibited by GABA, but the most potent inhibitors were hypotaurine and 2-guanidinoethanesulphonic acid. L-2,4-Diaminobutyric acid was moderately active. The uptake systems for taurine and beta-alanine were thus in principle similar, and they exhibited certain characteristics typical for a neurotransmitter amino acid. The inhibition studies further suggest the existence of only one common transport system for taurine, beta-alanine, and GABA in cultured primary astrocytes. The same uptake system may also be used for hypotaurine.
D000409:Alanine / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D001253:Astrocytes / Q000378:metabolism*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D018756:GABA Antagonists; D007700:Kinetics; D051381:Rats; D012964:Sodium / Q000494:pharmacology; D013654:Taurine / Q000031:analogs & derivatives / Q000037:antagonists & inhibitors / Q000378:metabolism*; D015091:beta-Alanine / Q000037:antagonists & inhibitors / Q000378:metabolism*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
D018756:GABA Antagonists; D015091:beta-Alanine; D013654:Taurine; D005680:gamma-Aminobutyric Acid; C003949:hypotaurine; D012964:Sodium; D000409:Alanine