Chemical induction of sister-chromatid exchanges in human lymphocytes treated in G0 prior to stimulation by different mitogens and revealed 72 h later in second division cells.
Frequencies of sister-chromatid exchanges (SCE) were determined in second-division metaphases of human lymphocytes, exposed for 1 h during the G0 phase to mitomycin C (MMC) alone or to cyclophosphamide (CP) in the presence of S9 mix. The cells were then cultured for 72 h in the presence of phytohemagglutinin (PHA), concanavalin A (Con A), Wistaria floribunda (WFA) or Lens culinaris (LcH-A) extracts. Large differences in mitotic indices (MI) and cell-cycle kinetics were observed among cells subjected to the various treatments. However, in the controls as well as in the cultures submitted to a G0 mutagenic exposure, the yield of SCE was not influenced by the mitogenic agent and was, therefore, independent of the proliferation properties of the cultured lymphocyte population.
D002453:Cell Cycle; D003520:Cyclophosphamide / Q000494:pharmacology; D004334:Drug Administration Schedule; D006801:Humans; D066298:In Vitro Techniques; D037102:Lectins / Q000008:administration & dosage; D008297:Male; D008934:Mitogens / Q000008:administration & dosage; D016685:Mitomycin; D008937:Mitomycins / Q000494:pharmacology; D009153:Mutagens / Q000008:administration & dosage*; D012854:Sister Chromatid Exchange / Q000187:drug effects*
D037102:Lectins; D008934:Mitogens; D008937:Mitomycins; D009153:Mutagens; D016685:Mitomycin; D003520:Cyclophosphamide