Total parenteral feeding of rats with an acetoacetate monoglyceride and glucose mixture.
Total parenteral nutrition (TPN) by means of monoacetoacetin (glycerol monoacetoacetate) was compared with TPN by using glucose-glycerol. Growth and urinary nitrogen, copper and zinc over 7 d and leucine dynamics on the last day were studied. Complete intravenous diets were administered to five groups of rats which were differentiated by receiving 45 kcal/d (group A) or 65 kcal/d (group C) from a 50% glucose-50% glycerol mixture, 45 kcal/d (group B) or 65 kcal/d (group D) from a 67% monoacetoacetin-33% glucose mixture, or 45 kcal/d (group E) from 100% monoacetoacetin. Leucine kinetics were determined by continuous infusion. Animals from groups A and C were hyperglycemic and normoketonemic, groups B and D were normoglycemic and hyperketonemic, and group E tended to be normoglycemic and hyperketonemic. Group B rats gained weight and retained the most nitrogen while groups A and E lost weight and groups C and D maintained their weight. Nitrogen losses correlated with weight changes. Urinary copper and zinc were not increased by giving ketone bodies. Leucine kinetics were found to be low for group C compared to its energy-matched group, and leucine metabolism was not correlated with nitrogen output and growth. The data suggest that a monoacetoacetin-glucose mixture is preferable as a nonprotein energy source for TPN when compared to either substance alone.
D000818:Animals; D001786:Blood Glucose; D001835:Body Weight; D002210:Caprylates; D002250:Carbon Radioisotopes; D003300:Copper / Q000652:urine; D002149:Energy Intake; D005947:Glucose / Q000008:administration & dosage / Q000378:metabolism*; D005989:Glycerides / Q000008:administration & dosage / Q000378:metabolism*; D005990:Glycerol / Q000378:metabolism*; D007263:Infusions, Parenteral; D007657:Ketone Bodies / Q000097:blood; D007700:Kinetics; D007930:Leucine / Q000378:metabolism; D008297:Male; D009584:Nitrogen / Q000652:urine; D010289:Parenteral Nutrition, Total; D051381:Rats; D011919:Rats, Inbred Strains; D015032:Zinc / Q000652:urine
D001786:Blood Glucose; D002210:Caprylates; D002250:Carbon Radioisotopes; D005989:Glycerides; D007657:Ketone Bodies; D003300:Copper; D007930:Leucine; D005947:Glucose; D015032:Zinc; D009584:Nitrogen; D005990:Glycerol; C025343:monooctanoin