Membrane-associated interleukin 1 is required for the activation of T cells in the anti-CD3 antibody-induced T cell response.
To establish the role of the membrane-associated form of IL 1 in T cell activation, we tested the accessory function of monocytes (Mo) that were unable to secrete or release soluble IL 1. The data obtained show that strictly purified resting T cells start to proliferate in response to anti-CD3 antibody (OKT3) stimulation in the presence of paraformaldehyde (PFA)-fixed Mo or 3-day cultured Mo (macrophages). The findings that i) PFA-fixed Mo did not produce or release IL 1, ii) the accessory function of PFA-fixed Mo could be inhibited with pretreatment with anti-IL 1 antibody, iii) PFA-fixed Mo had a comitogenic effect in the murine thymocyte assay, and iv) there was a temporal difference between the capacities to function in the comitogenic assay and to produce soluble IL 1, suggest that human Mo can express membrane-associated IL 1 and that it is functionally relevant.
D000911:Antibodies, Monoclonal; D000937:Antigen-Antibody Reactions; D000938:Antigen-Presenting Cells / Q000276:immunology*; D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface / Q000502:physiology*; D002462:Cell Membrane / Q000502:physiology; D005404:Fixatives; D006801:Humans; D007375:Interleukin-1 / Q000502:physiology*; D008213:Lymphocyte Activation; D009000:Monocytes / Q000502:physiology*; D011948:Receptors, Antigen, T-Cell / Q000502:physiology*; D012995:Solubility; D013601:T-Lymphocytes / Q000502:physiology*
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface; D005404:Fixatives; D007375:Interleukin-1; D011948:Receptors, Antigen, T-Cell