Replication of fungi in K-Sol corneal preservation medium at 4 degrees C.
We examined the growth of six fungi during 18 days at 4 degrees C in a corneal preservation medium containing 2.5% chondroitin sulfate (K-Sol). The number of viable organisms decreased with time for all six fungi, the average decrease being 65%. The risk of transplanting fungal organisms with a donor cornea stored for up to 18 days at 4 degrees C in preservation media containing chondroitin sulfate is no greater than that with the same cornea stored for only one day.
D001230:Aspergillus / Q000254:growth & development; D002175:Candida / Q000254:growth & development; D002809:Chondroitin Sulfates; D003315:Cornea; D004866:Equipment Contamination; D005615:Freezing; D005658:Fungi / Q000254:growth & development*; D006801:Humans; D012996:Solutions / Q000592:standards*; D014021:Tissue Preservation / Q000379:methods*
D012996:Solutions; D002809:Chondroitin Sulfates