Presence of interleukin 3-like activity in the supernatants of lipopolysaccharide-stimulated mouse splenocytes.
An Interleukin 3 (IL 3) activity was found in the supernatants of mouse spleen cells stimulated by lipopolysaccharide (LPS). The IL 3 activity was maximum in the supernatants of 5 to 7 days cultures. IL 3 activity was assessed by the capacity of the spleen cell supernatants to allow the growth of the FDC-P2 cell line, a strictly IL 3 dependent cell line. Polymyxin B could inhibit the IL 3 induction by LPS.
D000818:Animals; D002478:Cells, Cultured; D007377:Interleukin-3; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008222:Lymphokines / Q000096:biosynthesis*; D051379:Mice; D008807:Mice, Inbred BALB C; D009345:Neisseria meningitidis; D011112:Polymyxin B / Q000494:pharmacology; D013154:Spleen / Q000378:metabolism*; D013997:Time Factors
D007377:Interleukin-3; D008070:Lipopolysaccharides; D008222:Lymphokines; D011112:Polymyxin B