Isolation and structure of the Streptococcus faecalis sex pheromone, cAM373.
The Streptococcus faecalis sex pheromone, cAM373, which induces a mating response of donor cells harboring plasmid pAM373 and is also produced by Staphylococcus aureus, was isolated and its structure determined. Supernatant from an overnight culture of a recipient strain was subjected to successive purification procedures, and 4.4 micrograms cAM373 was obtained. The isolated pheromone showed activity at a concentration as low as 5 X 10(-11) M. Sequence analysis indicated that cAM373 was a heptapeptide, H-Ala-Ile-Phe-Ile-Leu-Ala-Ser-OH, and that its Mr was 733. A synthetic replicate of the peptide showed the same biological activity and chromatographic behavior as the native cAM373.
D000595:Amino Acid Sequence; D013293:Enterococcus faecalis / Q000032:analysis*; D009842:Oligopeptides / Q000302:isolation & purification*; D010675:Pheromones
D009842:Oligopeptides; D010675:Pheromones; C049695:sex pheromone cAM373, Streptococcus faecalis