title: DNA binding is not sufficient for nuclear localization of regulatory proteins in Saccharomyces cerevisiae.
abstract: We showed by immunofluorescence that the procaryotic DNA-binding protein LexA and a chimeric protein that contains the DNA-binding portion of LexA (amino acids 1 to 87) and a large portion (amino acids 74 to 881) of the Saccharomyces cerevisiae positive regulatory GAL4 protein (GAL4 gene product) are not preferentially localized in the nucleus in S. cerevisiae.
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D005796:Genes; D005800:Genes, Fungal; D005809:Genes, Regulator; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D012697:Serine Endopeptidases; D001616:beta-Galactosidase / Q000235:genetics; D000099160:LexA Repressor Protein
chemical_list: D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D012697:Serine Endopeptidases; D001616:beta-Galactosidase; D000099160:LexA Repressor Protein