title: Ruminal evacuation's effect on microbial activity and ruminal function.
abstract: The influence of evacuating, mixing and returning ruminal contents on microbial populations, volatile fatty acid (VFA) concentrations and liquid flow rate was investigated with four ruminal-cannulated Hereford steers (247 kg avg wt). Ruminoreticular contents were sampled, then completely removed, mixed for 5 min and returned to the rumen. Subsequent samples were taken immediately, 1 h and 4 h later. Non-evacuated steers were sampled at identical time intervals either 1 d before or after evacuation. Averaged over time, there was no significant difference between evacuated and non-evacuated steers in total anaerobic, cellulolytic and facultative bacteria, protozoa, oxidation-reduction potential, VFA concentrations, and liquid flow rates. There were no treatment X time interactions and, except for holotrich protozoa and VFA, no differences from time of sampling. Ruminal evacuation does not appear disruptive to anaerobiosis or detrimental to ruminal microorganisms and digestive processes.
mesh_terms: D000818:Animals; D002417:Cattle / Q000378:metabolism / Q000382:microbiology / Q000502:physiology*; D016798:Ciliophora; D004063:Digestion; D005232:Fatty Acids, Volatile / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008297:Male; D012417:Rumen / Q000378:metabolism / Q000382:microbiology / Q000469:parasitology / Q000502:physiology*
chemical_list: D005232:Fatty Acids, Volatile