title: A lactate dehydrogenase-immunoglobulin G1 complex, not blocked by anti-idiotype antibody, in a patient with IgG1-lambda type M-proteinemia.
abstract: The serum of a patient with IgG1-lambda type M-proteinemia showed an abnormal isoenzyme pattern for lactate dehydrogenase (LDH, EC 1.1.1.27). By affinity chromatography, we showed that four isoenzymes (LDH2, LDH3, LDH4, and LDH5) were bound to the M-protein. This complex formation was not blocked by anti-idiotype antibody, even though the binding capacity of IgG was exclusively located in the Fab region of the molecule. Moreover, heavy and light chains of the patient's IgG, obtained by reduction, separately had affinities for each of the LDH isoenzymes. LDH-IgG complex was easily dissociated by affinity chromatography on 5'-AMP-Sepharose 4B or by added NADH. We propose the following hypothesis for the LDH-IgG complex formation: LDH can recognize the gamma-Fab region of IgG at the NAD+ binding site of the molecule, but the affinity of the LDH molecule for immunoglobulin is much weaker than that for NADH or 5'-AMP.
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000915:Antibody Affinity; D001797:Blood Protein Electrophoresis; D002846:Chromatography, Affinity; D004587:Electrophoresis, Agar Gel; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology*; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D007146:Immunoglobulin lambda-Chains / Q000276:immunology*; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000276:immunology* / Q000378:metabolism; D008297:Male; D010265:Paraproteinemias / Q000201:enzymology / Q000276:immunology*
chemical_list: D007074:Immunoglobulin G; D007130:Immunoglobulin Idiotypes; D007146:Immunoglobulin lambda-Chains; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase