Isolation and characterization of apolipoproteins A-I, A-II, and A-IV.
A number of different analytical techniques are now available for the isolation of apoA-I, apoA-II, and apoA-IV. The choice of a particular technique is dependent on the instrumentation available, and the quantity of isolated apolipoprotein required. The isolation and characterization of the separate isoforms and the precursor isoproteins of the individual apolipoproteins are detailed, and methods for the evaluation of the purity of the separate apolipoproteins presented. A method for the evaluation of apolipoproteins in plasma is now available which permits the identification of structural variants of plasma apolipoproteins in patients with dyslipoproteinemias.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D016632:Apolipoprotein A-I; D016633:Apolipoprotein A-II; D001054:Apolipoproteins A / Q000097:blood* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004285:Dogs; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D006801:Humans; D005779:Immunodiffusion; D007525:Isoelectric Focusing / Q000379:methods; D008075:Lipoproteins, HDL / Q000097:blood*; D011110:Polymorphism, Genetic
D000596:Amino Acids; D016632:Apolipoprotein A-I; D016633:Apolipoprotein A-II; D001054:Apolipoproteins A; D008075:Lipoproteins, HDL; C022205:apolipoprotein A-IV