Preliminary investigations of a correlation between electron energy loss and morphometric analyses on ultrathin cryosections from normal and neoplastic gastric tissues.
Electron energy loss spectroscopy (EELS) has been used to measure the ratios of C, N, O, P and Ca in ultrathin cryosections from normal and neoplastic gastric tissues. First results show a correlation between the EELS, and morphometric data in cells from these tissues. We have found that ultrathin, freeze-dried cryosections, with an average thickness of up to 75 nm, are stable enough for EELS-analysis in a 200 KV electron microscope with an adapted Gatan-EELS-Spectrometer.
D000230:Adenocarcinoma / Q000378:metabolism* / Q000473:pathology / Q000648:ultrastructure; D002118:Calcium / Q000378:metabolism; D002244:Carbon / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D004583:Electrons; D004734:Energy Metabolism; D005756:Gastritis / Q000378:metabolism / Q000473:pathology; D006652:Histological Techniques; D006801:Humans; D008854:Microscopy, Electron; D009584:Nitrogen / Q000378:metabolism; D010758:Phosphorus / Q000378:metabolism; D013057:Spectrum Analysis / Q000379:methods*; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000473:pathology / Q000648:ultrastructure; D014018:Tissue Distribution
D010758:Phosphorus; D002244:Carbon; D009584:Nitrogen; D002118:Calcium