Lethal potency and tissue distribution of 125I-labelled toxic peptides from the blue-green alga Microcystis aeruginosa.
Toxic heptapeptides from a water bloom of the cyanobacterium Microcystis aeruginosa were purified by HPLC. The unoxidised fraction was iodinated with 125I plus 127I by the lactoperoxidase/H2O2 method, further purified by HPLC, and the non-iodinated and three iodinated fractions administered i.p. to male mice. All iodinated fractions were toxic, with symptoms and pathological lesions of the liver identical with those caused by non-iodinated peptide. Radioactivity was concentrated in the liver of mice at death.
D000818:Animals; D001427:Bacterial Toxins; D002851:Chromatography, High Pressure Liquid; D000087522:Cyanobacteria Toxins; D007457:Iodine Radioisotopes; D008099:Liver / Q000187:drug effects* / Q000473:pathology; D008297:Male; D008387:Marine Toxins / Q000302:isolation & purification* / Q000378:metabolism; D051379:Mice; D052998:Microcystins; D046931:Microcystis; D010455:Peptides / Q000302:isolation & purification* / Q000378:metabolism; D014018:Tissue Distribution
D001427:Bacterial Toxins; D000087522:Cyanobacteria Toxins; D007457:Iodine Radioisotopes; D008387:Marine Toxins; D052998:Microcystins; D010455:Peptides