Effect of recombinant human interferon gamma and interleukin 2 on CFU-GM.
Human bone marrow CFU-GM were cultured with rHu-GM-CSF and varying concentrations of rHu-gamma-IFN. Concentration-dependent suppression of CFU-GM precursors by gamma-IFN was demonstrated. This suppression was, in part, reversible by addition of rHu-IL-2. Reversal was also concentration-dependent. Both immune-regulatory T-cell mediators seem to act on a more primitive stage of committed progenitor cell development.
D001854:Bone Marrow Cells; D002449:Cell Aggregation; D002455:Cell Division; D002478:Cells, Cultured; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D007371:Interferon-gamma / Q000502:physiology*; D007376:Interleukin-2 / Q000502:physiology*; D011994:Recombinant Proteins / Q000502:physiology*
D007376:Interleukin-2; D011994:Recombinant Proteins; D007371:Interferon-gamma