Protection by uridine diphosphoglucuronic acid and DT-diaphorase against the cytotoxicity of polycyclic aromatic hydrocarbons isolated from a complex coal gasification condensate.
The cytotoxicities of polycyclic aromatic hydrocarbon (PAH) subclasses isolated from a complex organic mixture (coal gasification condensate) were studied in vitro in Chinese hamster ovary cells, in the presence of rat liver microsomes from animals pretreated with Aroclor. Toxicity was enhanced by microsomal metabolism and was inversely related to aromatic ring number. Rat liver cytosol, semipurified DT-diaphorase, and uridine diphosphoglucuronic acid decreased the cytotoxicity of a variety of PAH mixtures and representative PAH, as well as individual PAH metabolites. The results indicate that the in vitro toxicity of complex PAH mixtures is caused primarily by hydroxy-PAH and quinone metabolites of the predominant, nonmutagenic two- and three-ring PAHs.
D000818:Animals; D003031:Coal; D006224:Cricetinae; D005260:Female; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects*; D009152:Mutagenicity Tests; D016660:NAD(P)H Dehydrogenase (Quinone); D009281:Naphthalenes; D010053:Ovary / Q000187:drug effects*; D011083:Polycyclic Compounds; D011808:Quinone Reductases / Q000627:therapeutic use*; D051381:Rats; D011919:Rats, Inbred Strains; D013329:Structure-Activity Relationship; D014535:Uridine Diphosphate Glucuronic Acid / Q000627:therapeutic use*; D014539:Uridine Diphosphate Sugars / Q000627:therapeutic use*
D003031:Coal; D009281:Naphthalenes; D011083:Polycyclic Compounds; D014539:Uridine Diphosphate Sugars; D014535:Uridine Diphosphate Glucuronic Acid; D016660:NAD(P)H Dehydrogenase (Quinone); D011808:Quinone Reductases