Contribution of lipoxygenase metabolites to IL-2 production in the early phase of lymphocyte activation.
Lipoxygenase inhibitors; eicosatetraynoic acid (ETYA), esculetin and caffeic acid, inhibited interleukin-2 (IL-2) production by Con A (concanavalin A)-stimulated murine spleen lymphocytes. They effectively inhibited IL-2 production without affecting cell viability when they were added to the cultures within around 6 hr after Con A stimulation. IL-1 production was also inhibited effectively when the inhibitors were added within 4 hr after stimulation. Therefore, immunosuppressive characteristics of these inhibitors can in part be explained by their inhibitory effects on production of lymphokines involved in the early phase of lymphocyte activation.
D015117:5,8,11,14-Eicosatetraynoic Acid / Q000494:pharmacology; D000818:Animals; D001095:Arachidonic Acids / Q000378:metabolism* / Q000502:physiology; D002109:Caffeic Acids / Q000494:pharmacology; D003208:Concanavalin A / Q000494:pharmacology; D005260:Female; D007375:Interleukin-1 / Q000096:biosynthesis; D007376:Interleukin-2 / Q000096:biosynthesis*; D008084:Lipoxygenase / Q000378:metabolism*; D016859:Lipoxygenase Inhibitors; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D014468:Umbelliferones / Q000494:pharmacology
D001095:Arachidonic Acids; D002109:Caffeic Acids; D007375:Interleukin-1; D007376:Interleukin-2; D016859:Lipoxygenase Inhibitors; D014468:Umbelliferones; D003208:Concanavalin A; D015117:5,8,11,14-Eicosatetraynoic Acid; D008084:Lipoxygenase; C007628:esculetin; C040048:caffeic acid