Temporal effects on antipyrine metabolite kinetics in Aroclor 1254-treated rats.
The in vivo consequences of a single dose of Aroclor 1254 (50 mg/kg) on the drug metabolizing capacity of rats were investigated. A noninvasive method, employing [N-methyl-14C]-antipyrine where both 14CO2 exhalation and urinary excretion of 4-hydroxy-, 3-hydroxymethyl-, and norantipyrine were monitored, was used. A group of rats were sequentially tested over a 3-week period to characterize temporal patterns. The antipyrine metabolite kinetic approach demonstrated that induction of hepatic cytochrome P-450 is maximal 3-6 days after Aroclor 1254 administration and the effects were apparent for at least a further 14-17 days. Evidence is presented to suggest selective effects of Aroclor 1254 on different cytochromes P-450 are apparent in vivo.
D000818:Animals; D000983:Antipyrine / Q000031:analogs & derivatives / Q000378:metabolism* / Q000652:urine; D001140:Aroclors; D001944:Breath Tests; D002245:Carbon Dioxide / Q000032:analysis; D002250:Carbon Radioisotopes; D020111:Chlorodiphenyl (54% Chlorine); D002851:Chromatography, High Pressure Liquid; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis; D000077553:Edaravone; D004790:Enzyme Induction; D006207:Half-Life; D007274:Injections, Intraperitoneal; D007700:Kinetics; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D001140:Aroclors; D002250:Carbon Radioisotopes; D020111:Chlorodiphenyl (54% Chlorine); D002245:Carbon Dioxide; C007983:4-hydroxyantipyrine; C031820:3-hydroxymethylantipyrine; D003577:Cytochrome P-450 Enzyme System; D000077553:Edaravone; D000983:Antipyrine