Isolation and partial characterization of the reduction-modifiable protein of Neisseria gonorrhoeae.
We have isolated and purified the reduction-modifiable protein (protein III) from several strains of Neisseria gonorrhoeae. We have found them to be basic proteins (pI 8.6) and virtually identical. A similar but not identical protein was isolated from a meningococcal strain. Antibodies raised against this purified protein would absorb to whole bacteria, confirming that the protein is exposed on the surface of the organism.
D000596:Amino Acids / Q000032:analysis; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification*; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D009344:Neisseria gonorrhoeae / Q000032:analysis*; D010084:Oxidation-Reduction
D000596:Amino Acids; D001425:Bacterial Outer Membrane Proteins