Formation of lipoxin B by the pure reticulocyte lipoxygenase.
The pure reticulocyte lipoxygenase converts 5,15-DiHETE via a lipoxygenase reaction to 5,14,15-trihydroxy-6,8,10,12-eicosatetraenoic acid (a lipoxin B isomer) as shown by GC/MS analysis of its trimethylsilyl ether. With arachidonic acid, 15-HETE and 15-HETE methyl ester this lipoxin B isomer was also formed. The results presented here indicate that pure mammalian lipoxygenases are able to form lipoxins via sequential multiple oxygenation of arachidonic acid or its hydroxy derivatives.
D008401:Gas Chromatography-Mass Spectrometry; D006893:Hydroxyeicosatetraenoic Acids / Q000096:biosynthesis* / Q000378:metabolism; D007536:Isomerism; D044045:Lipoxins; D008084:Lipoxygenase / Q000378:metabolism*; D012156:Reticulocytes / Q000201:enzymology*; D013056:Spectrophotometry, Ultraviolet
D006893:Hydroxyeicosatetraenoic Acids; D044045:Lipoxins; C034922:5,15-dihydroxy-6,8,11,13-eicosatetraenoic acid; C042651:lipoxin B4; D008084:Lipoxygenase