Kinetic determination of enzymatic activity and modification of the metabolic activation system in the SOS chromotest.
The "SOS Chromotest" has recently been introduced by P. Quillardet et al. (1982; Quillardet and Hofnung, 1985), who use strain PQ37 of Escherichia coli K12 to test for genotoxicity. We have modified the procedure in order to optimize the determination of beta-galactosidase and alkaline phosphatase activities, and, where possible, to allow measurements to be made automatically. Kinetic determination is quicker, more sensitive and avoids interference by coloured compounds. Modification of the metabolic activation system increases the sensitivity of the test for progenotoxicity.
D000469:Alkaline Phosphatase / Q000235:genetics / Q000378:metabolism; D001711:Biotransformation; D004260:DNA Repair; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology / Q000235:genetics; D007700:Kinetics; D009152:Mutagenicity Tests / Q000379:methods; D009153:Mutagens / Q000494:pharmacology*; D009154:Mutation; D013014:SOS Response, Genetics; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
D009153:Mutagens; D000469:Alkaline Phosphatase; D001616:beta-Galactosidase