Putrescine does not mediate the androgen-response in mouse kidney.
We have tested the notion that polyamines, particularly putrescine, mediate the response of mouse kidney to androgens. Hormonal effects were measured in female mice maintained on the ornithine decarboxylase (ODC) inhibitor alpha-difluoromethylornithine (DFMO), which results in a 85-90% reduction of ODC enzyme levels and a depletion of putrescine concentrations in kidney. These animals exhibited normal kidney cell hypertrophy in response to testosterone. In addition, androgen-inducibility of the RP2 gene was indistinguishable from that in normal mice. These results indicate that an increase in putrescine levels is not a prerequisite for androgen effects in mouse kidney and that putrescine does not mediate the hormonal response.
D000818:Animals; D000518:Eflornithine; D005260:Female; D007668:Kidney / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D009952:Ornithine / Q000031:analogs & derivatives* / Q000494:pharmacology; D009955:Ornithine Decarboxylase / Q000235:genetics; D065108:Ornithine Decarboxylase Inhibitors; D011700:Putrescine / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D013095:Spermidine / Q000378:metabolism; D013096:Spermine / Q000378:metabolism; D013739:Testosterone / Q000494:pharmacology*
D065108:Ornithine Decarboxylase Inhibitors; D012333:RNA, Messenger; D013096:Spermine; D013739:Testosterone; D009952:Ornithine; D009955:Ornithine Decarboxylase; D013095:Spermidine; D011700:Putrescine; D000518:Eflornithine