A fluorometric assay for biotinidase.
An assay for biotinidase using biocytin, the natural substrate, is described. The fluorometric procedure uses 1,2-diacetylbenzene which reacts selectively with lysine allowing its direct determination in mixture with biocytin. We have examined the applicability of the assay using human serum biotinidase.
D000098:Acetophenones; D000581:Amidohydrolases / Q000032:analysis* / Q000097:blood; D043523:Biotinidase; D003124:Colorimetry; D006801:Humans; D008239:Lysine / Q000031:analogs & derivatives; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013379:Substrate Specificity
D000098:Acetophenones; C048792:1,2-diacetylbenzene; D000581:Amidohydrolases; D043523:Biotinidase; C013411:biocytin; D008239:Lysine