Cell distribution and antigenic properties of mammalian sarcolectins.
Sarcolectins are present in a great variety of tissues from mammalian origin. Such substances were observed to be secreted from cultures of human embryonic fibroblasts, human osteosarcoma and rat Rous sarcoma transformed cells and could be extracted from TG 180 Crocker Sarcoma or normal human placenta. All sarcolectins tested here, were comparable by their physicochemical properties to those previously reported in hamster or human sarcomas. Indeed, they are proteins or glycoproteins, resistant to pepsin and migrate in SDS-PAGE in the 65 kDa area. They agglutinate cells with an affinity for simple sugars and degrade previously established interferon-induced antiviral resistance. Considering the hamster sarcolectin as reference in this comparative study, both differences and similarities in the antigenic properties of mouse, rat and human sarcolectin variants were demonstrated. An indirect immunofluorescence assay showed that sarcolectins were specifically labelled on the cell surface but not detected in the cytoplasm after methanol or acetone permeabilization of the membrane. By electron microscopy, using immunoperoxidase labelling, sarcolectins can be localized on the surface of normal, transformed, human or rat cells. Only limited segments of normal cell membranes were labelled, while transformed cells were frequently stained on their whole surface. Other known extracellular proteins, such as fibronectin and collagen, did not share common antigenic determinants with sarcolectins.
D000818:Animals; D000936:Antigen-Antibody Complex / Q000032:analysis; D002460:Cell Line; D002478:Cells, Cultured; D005347:Fibroblasts / Q000032:analysis; D005455:Fluorescent Antibody Technique; D006801:Humans; D007106:Immune Sera; D005779:Immunodiffusion; D037102:Lectins / Q000032:analysis* / Q000276:immunology; D008854:Microscopy, Electron; D008970:Molecular Weight; D009124:Muscle Proteins / Q000032:analysis; D009363:Neoplasm Proteins / Q000032:analysis; D012516:Osteosarcoma; D012867:Skin
D000936:Antigen-Antibody Complex; D007106:Immune Sera; D037102:Lectins; D009124:Muscle Proteins; D009363:Neoplasm Proteins; C039495:sarcolectin