Studies on the kinetics of immobilized enzyme using a recycling enzyme reactor system.
A method of measuring kinetic parameters of immobilized enzyme with a recycling enzyme reactor system is described. By analyzing the plot of the dimensionless variable Ln(1-X)/X versus the time needed for a unit conversion, t/X, the mechanism of enzymatic reaction can be recognized and then its basic parameters can be evaluated. On the basis of the experimental data measured by P.R. Coulet et al., it has been proposed that the successive degradation of maltodextrins by the collagen membrane-bound amyloglucosidase was a product glucose inhibition reaction and their corresponding constants have been found with this method.
D001667:Binding, Competitive; D004800:Enzymes, Immobilized / Q000037:antagonists & inhibitors / Q000378:metabolism*; D005087:Glucan 1,4-alpha-Glucosidase / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D007700:Kinetics; D008433:Mathematics; D011134:Polysaccharides / Q000378:metabolism
D004800:Enzymes, Immobilized; D011134:Polysaccharides; C008315:maltodextrin; D005087:Glucan 1,4-alpha-Glucosidase; D005947:Glucose