Biosynthetic studies on N-acetylmannosaminuronic acid containing teichuronic acid in Bacillus megaterium.
The particulate enzymes obtained from four strains of Bacillus megaterium AHU 1240, AHU 1373, AHU 1375, and T catalyzed the synthesis of a polysaccharide and glycolipids from UDP-N-acetylmannosaminuronic acid, UDP-N-acetylglucosamine, and UDP-glucose. Chemical studies involving Smith degradation, acid hydrolysis, and N-acetylation revealed that the polysaccharide product has a backbone made up of trisaccharide repeating units comprising glucose, N-acetylmannosaminuronic acid, and N-acetylglucosamine and that the main oligosaccharide moieties of the glycolipids were identical with N-acetylmannosaminuronosyl-N-acetylglucosamine and glucosyl-N-acetylmannosaminuronosyl-N-acetylglucosamine. Incubation of the disaccharide-linked lipid with each particulate enzyme in the presence of UDP-glucose produced the trisaccharide-linked lipid and a polysaccharide. It is therefore suggested that in this polysaccharide-synthesizing system the repeating unit is formed on a carrier lipid from appropriate nucleotide derivatives first and the polymerization of the units then occurs to synthesize the backbone while the growing chain remains in pyrophosphate linkage to the carrier lipid presumed to be undecaprenol.
D000117:Acetylglucosamine / Q000378:metabolism; D001410:Bacillus megaterium / Q000201:enzymology / Q000378:metabolism*; D002854:Chromatography, Paper; D004590:Electrophoresis, Paper; D006017:Glycolipids / Q000096:biosynthesis*; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis*; D014532:Uridine Diphosphate Glucose / Q000378:metabolism; D014537:Uridine Diphosphate N-Acetylglucosamine / Q000378:metabolism; D014539:Uridine Diphosphate Sugars / Q000378:metabolism; D014574:Uronic Acids / Q000096:biosynthesis* / Q000378:metabolism*
D006017:Glycolipids; D011135:Polysaccharides, Bacterial; D014539:Uridine Diphosphate Sugars; D014574:Uronic Acids; C020949:uridine diphosphate N-acetyl-D-mannosaminuronic acid; C010788:teichuronic acid; D014537:Uridine Diphosphate N-Acetylglucosamine; C043028:N-acetylmannosaminuronic acid; D014532:Uridine Diphosphate Glucose; D000117:Acetylglucosamine