Evaluation of a commercially available direct immunofluorescent staining reagent for the detection of respiratory syncytial virus in respiratory secretions.
A commercially-available direct immunofluorescence (IF) reagent (Imagen; Boots-Celltech, Slough, Berkshire, United Kingdom) was similar in sensitivity and specificity to the conventional indirect IF test for the detection of respiratory syncytial virus in respiratory secretions. Both IF tests were more sensitive than culture, particularly for specimens transported from outside the institution.
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral / Q000276:immunology; D005069:Evaluation Studies as Topic; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D005455:Fluorescent Antibody Technique; D006801:Humans; D007202:Indicators and Reagents; D012136:Respiratory Syncytial Viruses / Q000145:classification*; D012137:Respiratory System / Q000382:microbiology; D012141:Respiratory Tract Infections / Q000382:microbiology*; D010253:Respirovirus Infections / Q000382:microbiology*; D013861:Thiocyanates
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D005452:Fluoresceins; D007202:Indicators and Reagents; D013861:Thiocyanates; D016650:Fluorescein-5-isothiocyanate