title: Occurrence in Bacillus megaterium of uridine 5'-diphospho-N-acetylgalactosamine and uridine 5'-diphosphogalactosamine, intermediates in the biosynthesis of galactosamine-6-phosphate polymer.
abstract: We isolated two galactosamine derivatives from Bacillus megaterium sporulating cells by lectin affinity chromatography followed by DEAE-Sephadex A-25 chromatography. From chemical analyses and measurements of these compounds, it was determined that one was uridine 5'-diphospho-N-acetylgalactosamine and that the other was uridine 5'-diphosphogalactosamine. They appeared in the middle stage of sporulation and disappeared during the period when galactosamine-6-phosphate is deposited on the forespore surface. These results suggest that uridine 5'-diphospho-N-acetylgalactosamine and uridine 5'-diphosphogalactosamine are intermediates in the biosynthesis of the galactosamine-6-phosphate polymer, a backbone structure of the exosporium.
mesh_terms: D001410:Bacillus megaterium / Q000254:growth & development*; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D013171:Spores, Bacterial / Q000502:physiology; D014536:Uridine Diphosphate N-Acetylgalactosamine / Q000031:analogs & derivatives / Q000302:isolation & purification*; D014539:Uridine Diphosphate Sugars / Q000302:isolation & purification*
chemical_list: D014539:Uridine Diphosphate Sugars; C051472:uridine diphosphate galactosamine; D014536:Uridine Diphosphate N-Acetylgalactosamine