Immunoglobulin synthesis in non-B cell lines.
A recombinant vector carrying rearranged immunoglobulin heavy (mu) and light (kappa) chain genes was introduced into several cell lines of non-B lineage including T cell (EL4) and two fibroblast lines (CV-1 and L cells). Our results indicated that intracytoplasmic mu-chains, both secretory and membrane types, were expressed in the recipient cell lines. Small amounts of kappa-chain transcripts, corresponding in size to authentic kappa-chain mRNA, were detected in EL4 and L cell transformants, whereas CV-1 transformants did not express the authentic kappa-chain RNA. EL4 and L cell transformants produced kappa-chain. This system will be quite useful for the analysis of regulatory mechanisms involved in immunoglobulin synthesis in non-B cells.
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D002460:Cell Line; D004247:DNA / Q000235:genetics; D005347:Fibroblasts / Q000276:immunology*; D007145:Immunoglobulin kappa-Chains / Q000096:biosynthesis / Q000235:genetics; D007148:Immunoglobulin mu-Chains / Q000096:biosynthesis / Q000235:genetics; D007136:Immunoglobulins / Q000096:biosynthesis* / Q000235:genetics; D007739:L Cells / Q000276:immunology; D051379:Mice; D010957:Plasmids; D013601:T-Lymphocytes / Q000276:immunology*; D014162:Transfection; D014170:Transformation, Genetic
D007145:Immunoglobulin kappa-Chains; D007148:Immunoglobulin mu-Chains; D007136:Immunoglobulins; D004247:DNA