A unique immune response control locus mapping with the H-2D class I region.
The immune response to the polysaccharide antigen Trinitrophenyl (TNP)-ficoll is controlled by two complementing loci in the murine major histocompatibility complex. One locus of control is present in the I-A subregion and the second is located between the S and D regions. A monoclonal antibody 48-21.7 was selected for its ability to significantly block the in vitro primary response to TNP-ficoll. This antibody bound to antigen-presenting cells does not interfere with the presentation of other antigens. Experiments are presented to demonstrate that the monoclonal antibody 48-21.7 precipitates a unique protein of approximately 40,000 daltons. Sequential immunoprecipitation with monoclonal antibodies to the class I Db antigen and to relevant Ia antigens demonstrated no cross-reactivity. Amino acid sequencing has found unique amino acids to be present at three class I N-terminal invariant positions. These observations indicate the presence of a unique class I-like molecule coded for by genes mapping between S and D and involved in the response to the polysaccharide ficoll.
D000818:Animals; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000235:genetics* / Q000276:immunology; D002846:Chromatography, Affinity; D002874:Chromosome Mapping; D004591:Electrophoresis, Polyacrylamide Gel; D005362:Ficoll / Q000031:analogs & derivatives / Q000276:immunology; D005802:Genes, MHC Class II; D006023:Glycoproteins / Q000235:genetics; D006183:H-2 Antigens / Q000235:genetics*; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D008285:Major Histocompatibility Complex; D051379:Mice; D014301:Trinitrobenzenes / Q000276:immunology
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D006023:Glycoproteins; D006183:H-2 Antigens; D000949:Histocompatibility Antigens Class II; C020529:TNP-ficoll; D014301:Trinitrobenzenes; D005362:Ficoll