Establishment and characterization of a human IgA-kappa-secreting plasma cell line (MT3).
We have established a new human plasma cell line from the peripheral blood of a patient with an IgA-kappa plasma-cell leukemia. Morphological, immunological, cytogenetic and molecular studies confirm that the cultured cells are derived from the same clone of leukemic plasma cell in vivo. The established cell line (MT3) grows in suspension, secretes high amounts of IgA kappa and exhibits morphological and ultrastructural characteristics of plasma cells. Surface marker analysis shows that both primary and cultured cells express the plasma-cell-associated antigens PCA-1 and T10, while specific B- and T-cell determinants and EBV nuclear antigen are undetectable. In the established cell line a few cells express Ia-like and CALLA antigens. Cytogenetic analysis of MT3 cells reveals a prevalent hypertriploid karyotype with constant chromosomal aberrations consisting of 14q+, 22q- and marker chromosomes.
D000954:Antigens, Surface / Q000032:analysis; D002460:Cell Line; D002869:Chromosome Aberrations; D004247:DNA / Q000032:analysis; D005260:Female; D006801:Humans; D007071:Immunoglobulin A, Secretory / Q000032:analysis*; D007145:Immunoglobulin kappa-Chains / Q000032:analysis* / Q000235:genetics; D007952:Leukemia, Plasma Cell / Q000235:genetics / Q000276:immunology / Q000473:pathology*; D008875:Middle Aged; D010950:Plasma Cells / Q000276:immunology / Q000378:metabolism*; D011995:Recombination, Genetic
D000954:Antigens, Surface; D007071:Immunoglobulin A, Secretory; D007145:Immunoglobulin kappa-Chains; D004247:DNA