Polysaccharide covalently linked to the peptidoglycan of the cyanobacterium Synechocystis sp. strain PCC6714.
A polysaccharide was found to be covalently linked to the peptidoglycan of the unicellular cyanobacterium Synechocystis sp. strain PCC6714 via phosphodiester bonds. It could be cleaved from the peptidoglycan-polysaccharide (PG-PS) complex by hydrofluoric acid (HF) treatment in the cold (48% HF, 0 degrees C, 48 h) yielding a pure, HF-insoluble peptidoglycan fraction and an HF-soluble polysaccharide fraction. The PG-PS complex was isolated from the Triton X-100-insoluble cell wall fraction by hot sodium dodecyl sulfate treatment and digestion with proteases. Digestion of the complex with N-acetylmuramidase released the glycopeptide-linked polysaccharide, which was further purified by dialysis and gel filtration on Sephadex G-50 and G-200. The polysaccharide consisted of glucosamine, mannosamine, galactosamine, mannose, and glucose and had a molecular weight of 25,000 to 30,000. Muramic acid-6-phosphate was identified as the binding site of the covalently linked, nonphosphorylated polysaccharide as revealed by chemical analysis of linkage fragments of the PG-PS complex.
D055598:Chemical Phenomena; D002621:Chemistry; D000458:Cyanobacteria / Q000032:analysis* / Q000378:metabolism; D019298:Glucose-6-Phosphate / Q000031:analogs & derivatives*; D005958:Glucosephosphates / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism; D008970:Molecular Weight; D009112:Muramic Acids / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism; D010457:Peptidoglycan / Q000032:analysis* / Q000378:metabolism; D011134:Polysaccharides / Q000032:analysis* / Q000378:metabolism; D013400:Sugar Acids / Q000032:analysis*
D005958:Glucosephosphates; D009112:Muramic Acids; D010457:Peptidoglycan; D011134:Polysaccharides; D013400:Sugar Acids; C050769:muramic acid-6-phosphate; D019298:Glucose-6-Phosphate