title: High-efficiency transformation of Streptococcus lactis protoplasts by plasmid DNA.
abstract: Streptococcus lactis IL1403 protoplasts were transformed by plasmid pIL204 (5.5 kilobases), which conferred erythromycin resistance with an average efficiency of 5 X 10(6) transformants per microgram of supercoiled DNA. The procedure used and transformation efficiencies obtained were close to those described for Bacillus subtilis (G. Chang and S. N. Cohen, Mol. Gen. Genet. 168:111-115, 1979).
mesh_terms: D004352:Drug Resistance, Microbial; D004917:Erythromycin / Q000494:pharmacology; D007700:Kinetics; D013294:Lactococcus lactis / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D011523:Protoplasts / Q000502:physiology*; D011815:R Factors; D014169:Transformation, Bacterial
chemical_list: D004917:Erythromycin