New method of preparing elastase toxoid from Pseudomonas aeruginosa.
A new method for the preparation of elastase toxoid of Pseudomonas aeruginosa was developed. A chloroacetyl peptide derivative (CICH2CO-HOLeu-Ala-Gly-NH2; HOLeu, N-hydroxy-L-leucine), an active-site-directed irreversible inhibitor of Pseudomonas aeruginosa elastase, was used to prepare elastase toxoid with or without pretreatment with Formalin and L-lysine. Elastase toxoid thus obtained appeared to be ideal, possessing negligible enzyme activity while retaining full antigenicity and immunogenicity.
D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology; D005557:Formaldehyde; D007114:Immunization; D008239:Lysine; D051379:Mice; D009842:Oligopeptides; D010196:Pancreatic Elastase / Q000037:antagonists & inhibitors / Q000276:immunology*; D011550:Pseudomonas aeruginosa / Q000201:enzymology* / Q000276:immunology; D011817:Rabbits; D014121:Toxoids / Q000276:immunology*
D000942:Antigens, Bacterial; D009842:Oligopeptides; D014121:Toxoids; D005557:Formaldehyde; C048705:chloroacetyl-N-hydroxyleucyl-alanyl-glycinamide; D010196:Pancreatic Elastase; D008239:Lysine