title: Pea (Pisum sativum) diamine oxidase contains pyrroloquinoline quinone as a cofactor.
abstract: Diamine oxidase was prepared from pea (Pisum sativum) seedlings by a new purification procedure involving two h.p.l.c. steps. We studied the optical and electrochemical properties of the homogeneous enzyme and also analysed the hydrolysed protein by several methods. The data presented here suggest that the carbonyl cofactor of diamine oxidase is firmly bound pyrroloquinoline quinone.
mesh_terms: D006631:Amine Oxidase (Copper-Containing) / Q000302:isolation & purification / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D003067:Coenzymes / Q000378:metabolism*; D007887:Fabaceae / Q000201:enzymology*; D045542:PQQ Cofactor; D010946:Plants, Medicinal; D011804:Quinolines / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013053:Spectrophotometry
chemical_list: D003067:Coenzymes; D011804:Quinolines; D045542:PQQ Cofactor; D006631:Amine Oxidase (Copper-Containing)