Cytochalasin B enhances T cell mitogenesis by promoting expression of an interleukin 2 receptor.
Low concentrations of cytochalasin B enhanced the T cell mitogenesis induced by concanavalin A (Con A) and interleukin 2 (IL-2). Mitogenesis was augmented by cytochalasin B given in the Con A-dependent early phase, or through T cell mitogenesis. Cytochalasin B did not enhance T cell mitogenesis when given only in the IL-2-dependent late phase. Use of the monoclonal antibody that directs the IL-2 receptor showed that cytochalasin B increased the expression of the IL-2 receptor induced by Con A. We concluded that cytochalasin b acts on an early phase of T cell mitogenesis and augments the expression of IL-2 receptor which enables certain nonresponsive T cells to respond to IL-2.
D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D003208:Concanavalin A; D003571:Cytochalasin B / Q000494:pharmacology*; D006801:Humans; D007376:Interleukin-2 / Q000276:immunology; D007700:Kinetics; D008213:Lymphocyte Activation / Q000187:drug effects*; D014066:Palatine Tonsil / Q000276:immunology; D011971:Receptors, Immunologic / Q000096:biosynthesis* / Q000187:drug effects; D015375:Receptors, Interleukin-2; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology*
D007376:Interleukin-2; D011971:Receptors, Immunologic; D015375:Receptors, Interleukin-2; D003208:Concanavalin A; D003571:Cytochalasin B