Thiolation of low-density lipoproteins and their interaction with L2C leukemic lymphocytes.
We present here, a new method for coupling sulfhydryl groups (SH) to low-density lipoprotein (LDL) surface. This method uses homocysteine thiolactone (HCTL) which reacts with lysine residues in a very mild manner, and permits the selection of the number of SH bound per LDL. Under our experimental conditions (8 SH/LDL), the affinity of thiolated LDL for the specific receptors and their further internalization by L2C lymphocytes are preserved.
D000818:Animals; D002460:Cell Line; D006168:Guinea Pigs; D006710:Homocysteine / Q000031:analogs & derivatives* / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D007942:Leukemia, Experimental / Q000378:metabolism*; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008214:Lymphocytes / Q000378:metabolism*; D008854:Microscopy, Electron; D011973:Receptors, LDL / Q000378:metabolism
D008077:Lipoproteins, LDL; D011973:Receptors, LDL; D006710:Homocysteine; C007957:homocysteine thiolactone