Expression of genes in cloned murine cell lines that can be maintained in both interleukin 2- and interleukin 3-dependent growth states.
Two cloned murine cell lines, FD.C/1 and 32Dcl-23 exhibit switching of lymphokine-dependent growth states. The bone marrow-derived FD.C/1 and 32Dcl-23 cell lines are normally grown in culture medium supplemented with interleukin 3 (IL3). The replacement of IL3 with interleukin 2 (IL2) in the medium results in an increase in IL2 receptor expression in FD.C/1 and 32Dcl-23 cells and the switching of cells to an IL2-dependent growth state. We have compared patterns of protein and phosphoprotein synthesis, as well as the expression of the c-abl, c-ras, c-myb, and c-fos oncogenes in these cell lines maintained in IL3- and IL2-dependent growth states. The synthesis of a series of proteins and phosphoproteins are identified with each of the lymphokine-dependent growth states. All of the oncogenes examined are expressed in both IL2- and IL3-dependent cells and are not altered by phenotypic changes in lymphokine growth dependence. The relationship of oncogene expression to intracellular pathways regulated by lymphokine-receptor interactions is considered.
D000818:Animals; D002455:Cell Division; D002460:Cell Line; D005786:Gene Expression Regulation; D007376:Interleukin-2 / Q000502:physiology*; D007377:Interleukin-3 / Q000502:physiology*; D051379:Mice; D009857:Oncogenes; D010750:Phosphoproteins / Q000096:biosynthesis; D014176:Protein Biosynthesis; D011971:Receptors, Immunologic / Q000096:biosynthesis; D015375:Receptors, Interleukin-2
D007376:Interleukin-2; D007377:Interleukin-3; D010750:Phosphoproteins; D011971:Receptors, Immunologic; D015375:Receptors, Interleukin-2