A benzo[a]pyrene metabolite-DNA adduct occurring in the activating cell but not in exogenous DNA.
[3H]Benzo[a]pyrene (BP) and salmon sperm DNA were incubated with hepatocytes from 5,6-benzo-flavone-treated rats. The cellular DNA and the exogenously added DNA were separately isolated, hydrolyzed and chromatographed on a Sephadex LH-20 column. The extracellular DNA yielded 3 peaks of radioactivity in the chromatographic eluate. The cellular DNA contained an additional peak suggesting the formation of a DNA adduct from a metabolite that does not leave the cell.
D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide / Q000378:metabolism; D000818:Animals; D001564:Benzo(a)pyrene / Q000378:metabolism*; D001580:Benzopyrenes / Q000378:metabolism; D001711:Biotransformation; D004247:DNA / Q000378:metabolism*; D008099:Liver / Q000166:cytology / Q000378:metabolism; D051381:Rats; D012474:Salmon
D001580:Benzopyrenes; C021337:9-hydroxybenzo(a)pyrene; D001564:Benzo(a)pyrene; C006707:benzo(a)pyrene 4,5-epoxide; D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide; D004247:DNA