Liquid chromatographic method for the determination of 3,4-dihydroxyphenylethylene glycol and 3,4-dihydroxymandelic acid in plasma.
A method is described for the simultaneous determination of the deaminated catecholamine metabolites 3,4-dihydroxyphenylethylene glycol (DOPEG) and 3,4-dihydroxymandelic acid (DOMA) in plasma by liquid chromatography with amperometric detection. The compounds are extracted from plasma by adsorption on alumina, then separated on a reversed-phase column coated with tributyl phosphate as the stationary liquid phase. It is a simple and selective method that permits the determination of basal levels of DOPEG and DOMA in plasma with a relative standard deviation of 3%.
D002853:Chromatography, Liquid; D004355:Drug Stability; D004492:Edetic Acid; D005978:Glutathione; D006018:Glycols / Q000097:blood*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008333:Mandelic Acids / Q000097:blood*; D008734:Methoxyhydroxyphenylglycol / Q000031:analogs & derivatives / Q000097:blood*; D013696:Temperature
D006018:Glycols; D008333:Mandelic Acids; D008734:Methoxyhydroxyphenylglycol; C000989:3,4-dihydroxymandelic acid; D004492:Edetic Acid; D005978:Glutathione; C010117:3,4-dihydroxyphenylglycol