title: Assembly of D-alanyl-lipoteichoic acid in Lactobacillus casei: mutants deficient in the D-alanyl ester content of this amphiphile.
abstract: D-Alanyl-lipoteichoic acid (D-alanyl-LTA) from Lactobacillus casei ATCC 7469 contains a poly(glycerophosphate) moiety that is acylated with D-alanyl ester residues. The physiological function of these residues is not well understood. Five mutant strains of this organism that are deficient in the esters of this amphiphile were isolated and characterized. When compared with the parent, strains AN-1 and AN-4 incorporated less than 10% of D-[14C]alanine into LTA, whereas AN-2, AN-3, and AN-5 incorporated 50%. The synthesis of D-[14C]alanyl-lipophilic LTA was virtually absent in the first group and was approximately 30% in the second group. The mutant strains synthesized and selected the glycolipid anchor for LTA assembly. In addition, all of the strains synthesized the poly(glycerophosphate) moiety of LTA to the same extent as did the parent or to a greater extent. It was concluded that the membranes from the mutant strains AN-1 and AN-4 are defective for D-alanylation of LTA even though acceptor LTA is present. Mutant strains AN-2 and AN-3 appear to be partially deficient in the amount of the D-alanine-activating enzyme. Aberrant morphology and defective cell separation appear to result from this deficiency in D-alanyl ester content.
mesh_terms: D000409:Alanine / Q000378:metabolism; D002455:Cell Division; D002462:Cell Membrane / Q000378:metabolism; D006017:Glycolipids / Q000032:analysis / Q000096:biosynthesis; D007780:Lacticaseibacillus casei / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D009154:Mutation; D013682:Teichoic Acids / Q000096:biosynthesis*
chemical_list: D006017:Glycolipids; D013682:Teichoic Acids; C026244:alanyl-lipoteichoic acid; D000409:Alanine