Fluorescence resonance energy transfer between the nucleotide binding site and Cys-10 in G-actin and F-actin.
Intramonomer fluorescence resonance energy transfer between the donor epsilon-ATP bound to the nucleotide site and the acceptor N-(4-dimethylamino-3,5-dinitrophenyl)maleimide (DDPM) or 4-dimethylaminophenyl-azophenyl-4'-maleimide bound to Cys-10 in G-actin was measured. The donor-acceptor distance was calculated to be about 40 A. The intermonomer energy transfer in F-actin occurring between epsilon-ADP and DABMI was also measured. The radial coordinate of Cys-10 was calculated to be 25 A based on the helical symmetry of F-actin and the recently calculated radial coordinate of the nucleotide binding site in F-actin i.e. 25 A (Miki, M., Hambly, B. and dos Remedios, C.G. (1986) Biochim. Biophys. Acta 871, 137-141). (The assumption has been made in calculating these distances that the energy donor and acceptor rotate rapidly relative to the fluorescence lifetime.) Corresponding distances separating the donor nucleotide in one monomer from acceptors on Cys-10 in the first and second nearest neighbours in F-actin are 39-40 A and 41-43 A.
D000199:Actins / Q000378:metabolism*; D001665:Binding Sites; D001726:Bisacodyl / Q000031:analogs & derivatives / Q000378:metabolism; D003553:Cystine; D004735:Energy Transfer; D008433:Mathematics; D009711:Nucleotides / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D004124:p-Dimethylaminoazobenzene / Q000031:analogs & derivatives / Q000378:metabolism
D000199:Actins; D009711:Nucleotides; D001726:Bisacodyl; D003553:Cystine; C049748:4-dimethylaminophenylazophenyl-4'-maleimide; D004124:p-Dimethylaminoazobenzene; C001659:dihydroxydiphenyl-pyridyl methane