title: Pancreatic secretion in the rat influenced by the low molecular weight serine proteinase inhibitor Gabexate mesilate.
abstract: The effect of intravenous or intragastric administration of the synthetic proteinase inhibitor Gabexate mesilate (GM) on the pancreas of rats was investigated. Infused intravenously at 4 mg kg-1 h-1, GM inhibited both basal or cerulein (0.2 microgram kg-1 h-1)-stimulated pancreatic protein secretion. Intracellular transport and secretion of newly synthesized pancreatic enzymes was not influenced by intravenous infusion of GM. Intragastric administration of GM (400 mg kg-1) on four consecutive days increased pancreatic wet weight, protein and enzyme content of the gland. A preferential increase of proteinases above glucosidases was observed. Pancreatic lobules from inhibitor-treated rats released 30% less amylase in response to cerulein or carbachol when the rate of discharge was expressed in percent of initial content. Expressed in ku amylase/microgram DNA secretion rate was two-fold higher than in controls. In pancreatic duct cannulated rats GM (400 mg kg-1 h-1), introduced intragastrically on five consecutive days, stimulated volume-bicarbonate and protein secretion rate, the secretory response on the fifth day being significantly higher than on the first day. Enzyme pattern in pancreatic juice changed characteristically: mainly the amount of acidic proteinases increased, whereas the amount of the basic isoforms was altered only slightly.
mesh_terms: D000818:Animals; D002766:Cholecystokinin / Q000494:pharmacology; D004798:Enzymes / Q000378:metabolism; D005260:Female; D016670:Gabexate; D006146:Guanidines / Q000008:administration & dosage / Q000494:pharmacology*; D007262:Infusions, Intravenous; D007322:Instillation, Drug; D010179:Pancreas / Q000187:drug effects* / Q000378:metabolism; D011480:Protease Inhibitors / Q000494:pharmacology; D011506:Proteins / Q000378:metabolism; D051381:Rats; D013270:Stomach
chemical_list: D004798:Enzymes; D006146:Guanidines; D011480:Protease Inhibitors; D011506:Proteins; D016670:Gabexate; D002766:Cholecystokinin