title: A new solid-phase assay for biotin and biocytin and its application to the study of patients with biotinidase deficiency.
abstract: New solid-phase assays for the determination of biotin in plasma and urine and the determination of biocytin/biotinyl peptides in urine are described. These assays were used to demonstrate that patients with biotinidase deficiency excrete increased amounts of biocytin/biotinyl peptides.
mesh_terms: D000581:Amidohydrolases / Q000172:deficiency*; D001681:Biological Assay; D001710:Biotin / Q000097:blood* / Q000652:urine; D043523:Biotinidase; D019411:Clinical Laboratory Techniques; D006801:Humans; D008239:Lysine / Q000031:analogs & derivatives* / Q000097:blood / Q000652:urine; D008661:Metabolism, Inborn Errors / Q000175:diagnosis*; D011869:Radioligand Assay
chemical_list: D001710:Biotin; D000581:Amidohydrolases; D043523:Biotinidase; C013411:biocytin; D008239:Lysine