Study on the pre-haemolytic complex formed upon polyethylene glycol-induced activation of mouse complement.
The stability and nature of haemolytic activity generated in normal mouse serum upon precipitation with a critical amount of polyethylene glycol and incubation of the redissolved precipitate in the presence of 10 mM EDTA were investigated. The activity appeared to be stable at 0 degrees C which enabled further analysis. The haemolytic mixture was subfractionated by repeated differential polyethylene glycol precipitation yielding two preparations with little or no haemolytic activity by themselves, but which regained activity upon recombination. Material precipitating between 4 and 9% polyethylene glycol became haemolytic when combined with C5-deficient serum. The ultracentrifugation profile of this subfraction strongly suggested the presence of C56-complexes.
D000818:Animals; D003167:Complement Activation / Q000187:drug effects*; D003182:Complement C5 / Q000502:physiology; D004492:Edetic Acid / Q000494:pharmacology; D005260:Female; D006461:Hemolysis / Q000187:drug effects*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008815:Mice, Inbred Strains; D011092:Polyethylene Glycols / Q000494:pharmacology*; D011817:Rabbits; D013696:Temperature; D014461:Ultracentrifugation
D003182:Complement C5; D011092:Polyethylene Glycols; D004492:Edetic Acid