Clinical application of the chromogenic assay of factor VIII in haemophilia A, and different variants of von Willebrand's disease.
A chromogenic substrate kit for determination of factor VIII activity (COATEST Factor VIII) was compared to a one-stage clotting assay and the correlation was evaluated in different genetic variants of mild and moderate haemophilia A, in severe haemophilia A and in all known variants of von Willebrand's disease. In all these cases a high correlation between the two methods was obtained. A good correlation was also obtained after intranasal administration of DDAVP (1-desamino-8-D-arginine vasopressin) to patients with von Willebrand's disease. The chromogenic substrate method was performed using a microtray technique.
D002863:Chromogenic Compounds; D003894:Deamino Arginine Vasopressin / Q000627:therapeutic use; D005169:Factor VIII / Q000032:analysis*; D006467:Hemophilia A / Q000097:blood* / Q000188:drug therapy / Q000503:physiopathology; D006801:Humans; D014842:von Willebrand Diseases / Q000097:blood* / Q000145:classification
D002863:Chromogenic Compounds; D005169:Factor VIII; D003894:Deamino Arginine Vasopressin