Histochemical demonstration of membrane-bound acetylcholinesterase: a methodological consideration on Koelle's copper thiocholine reaction and on Karnovsky's copper ferrocyanide reaction.
On the basis of own methodological investigations the reaction mechanisms of the methods of Koelle and Friedenwald (1949) and Karnovsky and Roots (1964) are described. Some modifications of these two methods are proposed.
D000109:Acetylcholine / Q000032:analysis; D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D002021:Buffers; D002462:Cell Membrane / Q000201:enzymology / Q000648:ultrastructure; D003300:Copper; D005069:Evaluation Studies as Topic; D005295:Ferrocyanides; D006651:Histocytochemistry / Q000379:methods; D007202:Indicators and Reagents; D008854:Microscopy, Electron; D013860:Thiocholine
D002021:Buffers; D005295:Ferrocyanides; D007202:Indicators and Reagents; D013860:Thiocholine; D003300:Copper; C039105:cupric ferrocyanide; D000110:Acetylcholinesterase; D000109:Acetylcholine