[Nuclear proteins preferentially bound to the beta-globin gene: cellular specificity and variation during terminal differentiation of mouse erythroblasts].
Restriction fragments of the mouse beta major globin gene and of the long terminal repeat (LTR) DNA fragment of the mouse mammary tumor provirus as a control, were used to analyze the specificity of DNA-protein interactions in nuclear extracts of mouse erythroleukemia (MEL) cells and of other differentiated mouse cultured cell lines. After gel electrophoresis and transfer to nitrocellulose, DNA-binding proteins with a preferential affinity for the cloned beta-globin genomic sequence were characterized and related to the level of globin gene expression during induction of differentiating mouse erythroblasts. Two proteins (110 K and 75 K) appear in differentiated MEL cells while another one (100 K), for which we have localized the binding site on the beta-globin gene, is present only in immature MEL cells.
D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D004247:DNA / Q000378:metabolism; D004900:Erythroblasts / Q000166:cytology / Q000378:metabolism*; D005796:Genes; D005914:Globins / Q000235:genetics*; D004915:Leukemia, Erythroblastic, Acute / Q000378:metabolism*; D007942:Leukemia, Experimental / Q000378:metabolism*; D051379:Mice; D009698:Nucleoproteins / Q000378:metabolism*; D011485:Protein Binding
D009698:Nucleoproteins; D005914:Globins; D004247:DNA
Protéines nucléaires se liant préférentiellement au gène beta-globine: spécificité cellulaire et variations au cours de la différenciation terminale des érythroblastes de souris.