Immunological study of ovarian inhibin.
Antisera to purified porcine follicular fluid inhibin of 32 K protein (pFF 32 K inhibin) were raised in rabbits. Increasing doses of an antiserum with high titer could neutralize the maximal suppression of FSH secretion caused by 10 ng of pFF 32 K inhibin from rat anterior pituitary cells in culture. A radioimmunoassay was developed using the antiserum and 125I-labelled pFF 32 K inhibin. Specificity of the antiserum was examined by comparing immunological and biological potencies of various inhibin preparations. Cross-reactivity tests revealed that the antiserum almost recognizes rat ovarian inhibin preparations. The antiserum also recognizes purified bovine follicular fluid inhibin of 32 K protein (bFF 32 K inhibin), but with a cross-reactivity of approximately 20%. Cross-reactivity of human follicular fluid to the antiserum was less than 10%. The antiserum also recognizes inhibin forms of higher molecular weights, 100 K, 80 K, and 55 K proteins, which have previously been identified by gel filtration or SDS-PAGE of crude pFF inhibin preparations under protein-dissociation conditions, indicating that these inhibin forms have common or closely related immunodetermining sites.
D000818:Animals; D000918:Antibody Specificity; D002478:Cells, Cultured; D003429:Cross Reactions; D004305:Dose-Response Relationship, Drug; D005260:Female; D005640:Follicle Stimulating Hormone / Q000378:metabolism; D006801:Humans; D007106:Immune Sera / Q000032:analysis; D007265:Inhibins / Q000276:immunology* / Q000302:isolation & purification; D007986:Luteinizing Hormone / Q000378:metabolism; D008970:Molecular Weight; D006080:Ovarian Follicle / Q000032:analysis; D010053:Ovary; D010902:Pituitary Gland / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D011817:Rabbits / Q000276:immunology; D011863:Radioimmunoassay; D051381:Rats; D013552:Swine
D007106:Immune Sera; D007265:Inhibins; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone