Inhibition of arachidonic acid oxidation by beta-carotene, retinol and alpha-tocopherol.
Prostaglandin and hydroxyeicosatetraenoic acid (HETE) production from arachidonic acid in bovine seminal vesicles and kidney as influenced by the addition of beta-carotene, retinol or alpha-tocopherol was studied. The major product formed was prostaglandin E2 (approximately 85% prostaglandin E2 of control), and its proportion decreased with increasing concentration of the additives, while the proportion of HETE increased. Prostaglandin and HETE production was considerably inhibited by beta-carotene and retinol, and to a lesser extent by alpha-tocopherol; HETE production was inhibited less than that of prostaglandin. It appears that beta-carotene, retinol and alpha-tocopherol influence both the cyclooxygenase and lipoxygenase pathways; this modulation of arachidonic acid oxidation by physiological compounds may have important in vivo implications.
D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism*; D002338:Carotenoids / Q000494:pharmacology*; D002417:Cattle; D006893:Hydroxyeicosatetraenoic Acids / Q000096:biosynthesis; D066298:In Vitro Techniques; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D008297:Male; D010084:Oxidation-Reduction; D011453:Prostaglandins / Q000096:biosynthesis; D012669:Seminal Vesicles / Q000187:drug effects / Q000378:metabolism; D014801:Vitamin A / Q000494:pharmacology*; D014810:Vitamin E / Q000494:pharmacology*; D019207:beta Carotene
D001095:Arachidonic Acids; D006893:Hydroxyeicosatetraenoic Acids; D011453:Prostaglandins; D019207:beta Carotene; D014801:Vitamin A; D014810:Vitamin E; D016718:Arachidonic Acid; D002338:Carotenoids