title: An enzyme-linked immunosorbent assay (ELISA) for detection of antibodies to Encephalitozoon cuniculi and its use in determination of infections in man.
abstract: An enzyme-linked immunosorbent assay (ELISA) was used to detect antibodies to Encephalitozoon cuniculi in man, using spores derived from tissue culture as antigen. Negligible cross-reactions were found with other microsporidia of vertebrate or of invertebrate origin and there was no cross-reaction with Toxoplasma gondii, using ELISA, immunoperoxidase or immunofluorescence staining. A high prevalence of antibodies to E. cuniculi was found in patients suffering from schistosomiasis, malaria and neurological and psychiatric disorders, but not in healthy individuals.
mesh_terms: D000818:Animals; D000906:Antibodies / Q000032:analysis*; D016782:Apicomplexa / Q000276:immunology*; D009670:Encephalitozoon cuniculi / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006801:Humans; D007124:Immunoenzyme Techniques; D008288:Malaria / Q000276:immunology; D001523:Mental Disorders / Q000276:immunology; D009422:Nervous System Diseases / Q000276:immunology; D011528:Protozoan Infections / Q000175:diagnosis* / Q000276:immunology; D051381:Rats; D012552:Schistosomiasis / Q000276:immunology
chemical_list: D000906:Antibodies