Immunoreactivity of the Rho(D) antigen in cytoskeleton-free vesicles.
Skeleton-free microvesicles derived from D-positive red cells immunospecifically bind anti-D. Anti-D saturation binding yielded an equilibrium constant value (K = 1-2 X 10(8) I mole-1) similar to intact red cells. The vesicles contained band 3, the major sialoglycoproteins and, at high protein loads, low-molecular-weight polypeptides. Anti-D binding was referenced to total protein, phospholipid, and band 3 content to determine whether there was nonrandom segregation of the D antigen into vesicles. Selective segregation of the D antigen into vesicles could not be demonstrated unequivocally based on the methods and the assumptions of this study. The results, however, indicate that D reactivity does not require a membrane skeletal association.
D001457:Anion Exchange Protein 1, Erythrocyte / Q000032:analysis / Q000276:immunology; D002451:Cell Compartmentation; D003599:Cytoskeleton / Q000502:physiology; D004910:Erythrocyte Membrane / Q000276:immunology*; D006801:Humans; D008565:Membrane Proteins / Q000097:blood / Q000276:immunology; D008970:Molecular Weight; D010743:Phospholipids / Q000097:blood / Q000276:immunology; D012204:Rh-Hr Blood-Group System / Q000276:immunology*
D001457:Anion Exchange Protein 1, Erythrocyte; D008565:Membrane Proteins; D010743:Phospholipids; D012204:Rh-Hr Blood-Group System