Metabolizable 111In chelate conjugated anti-idiotype monoclonal antibody for radioimmunodetection of lymphoma in mice.
The relative biological properties of 111In-labeled monoclonal antibodies (MoAb) coupled with a conventional bifunctional chelate (BC) and a new, enzyme metabolizable, bifunctional chelate (BCM) were investigated. A rat IgG2a MoAb against idiotype from a mouse B-cell lymphoma was utilized. Mice bearing B-cell lymphomas in the subcutaneous tissues of the flank were given IV-injections of labeled MoAb and imaged or killed for organ counting at 24 h or 48 h. Rat anti-dinitrophenyl IgG2a MoAb and non-specific polyclonal mouse IgG were used as controls. Compared to BC, the use of BCM resulted in a substantial decrease in blood background activity, a shorter biological half-life and an increase in tumor to blood ratio at the expense of a moderate decrease in absolute tumor uptake. The versatile chemistry of these C-1 substituted bifunctional chelates provides a variety of possible enzyme cleavable moieties for further investigation.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology; D002614:Chelating Agents; D004492:Edetic Acid / Q000031:analogs & derivatives* / Q000378:metabolism; D005029:Ethylenediamines / Q000378:metabolism; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D007204:Indium / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008223:Lymphoma / Q000000981:diagnostic imaging* / Q000276:immunology / Q000378:metabolism; D008657:Metabolic Clearance Rate; D051379:Mice; D008809:Mice, Inbred C3H; D011868:Radioisotopes / Q000378:metabolism; D011877:Radionuclide Imaging; D051381:Rats; D013154:Spleen / Q000378:metabolism; D013388:Succinimides / Q000378:metabolism
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D002614:Chelating Agents; D005029:Ethylenediamines; D007130:Immunoglobulin Idiotypes; D011868:Radioisotopes; D013388:Succinimides; D007204:Indium; C051801:ethyleneglycol bis(succinimidyl succinate)hydroxyethylethylenediamine triacetic acid; C042007:1-(4-bromoacetamidobenzyl)EDTA; D004492:Edetic Acid