Effects of chelates and incubation media on platelet labeling with indium-111.
We studied the effects of various [111In]chelates and incubation media on labeling efficiency (LE) and in vivo survival of platelets. High LE of human and rabbit platelets in plasma were obtained with [111In]tropolone and [111In]mercaptopyridine-N-oxide. Indium-111 oxine in plasma resulted in a moderate LE and required a longer incubation time, while [111In]oxine sulfate had low LE and inconsistent labeling. High LE for all forms of [111In]chelates were achieved in labeling media free of plasma. However, in vivo platelet survival in rabbits was markedly reduced when platelets were labeled in the absence of plasma.
D000818:Animals; D001792:Blood Platelets / Q000166:cytology; D002453:Cell Cycle; D002470:Cell Survival; D002614:Chelating Agents; D006801:Humans; D007204:Indium; D007553:Isotope Labeling / Q000379:methods*; D015125:Oxyquinoline; D011725:Pyridines; D011817:Rabbits; D011868:Radioisotopes; D013871:Thiones; D014018:Tissue Distribution; D014334:Tropolone
D002614:Chelating Agents; D011725:Pyridines; D011868:Radioisotopes; D013871:Thiones; D007204:Indium; D015125:Oxyquinoline; C008704:pyrithione; D014334:Tropolone