title: Separation of human pepsin and gastricsin by affinity chromatography with an immobilized synthetic inhibitor.
abstract: Pepsin and gastricsin from human gastric juice were separated by affinity chromatography on Sepharose 4B containing the immobilized synthetic inhibitor of aspartic proteinases, Val-D-Leu-Pro-Phe-Phe-Val-D-Leu. These enzymes were bound to the support at low pH, and gastricsin was released at the same pH with buffer containing 20% dioxan. Pepsin was not released under these conditions, but was eluted at higher pH with buffer also containing 20% dioxan. To obtain perfect separations, it is recommended to use diluted samples. Proteinases from the homogenate of human gastric mucosa are isolated on DEAE-cellulose before separation by affinity chromatography. Pepsin and gastricsin from human gastric juice and human gastric mucosa separated on DEAE-cellulose and isolated by affinity chromatography, were electrophoretically pure.
mesh_terms: D002846:Chromatography, Affinity; D004587:Electrophoresis, Agar Gel; D005750:Gastric Juice / Q000032:analysis; D005753:Gastric Mucosa / Q000201:enzymology; D006801:Humans; D010434:Pepsin A / Q000037:antagonists & inhibitors / Q000302:isolation & purification*; D014020:Tissue Extracts / Q000032:analysis
chemical_list: D014020:Tissue Extracts; D010434:Pepsin A; C020557:gastricsin