A monoclonal antibody to the heavy chain of human IgM with a particular reaction pattern in human B-cell malignancies.
We produced a monoclonal antibody to the human mu-chain. Its specificity was proved by amino acid-sequence analysis of immunoabsorbent purified cell surface antigen. In binding studies an affinity constant (K-aff) of 5.7 X 10(9) M-1 was determined. When compared with a polyclonal anti-IgM antiserum it was found that this antibody may react with a determinant of the mu-chain differently exposed in clonal B-cell disorders rendering it to a reagent of particular diagnostic interest.
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000276:immunology* / Q000302:isolation & purification; D000918:Antibody Specificity; D001402:B-Lymphocytes / Q000276:immunology; D001666:Binding Sites, Antibody; D005260:Female; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007114:Immunization; D007143:Immunoglobulin Heavy Chains / Q000276:immunology*; D007075:Immunoglobulin M / Q000276:immunology*; D007938:Leukemia / Q000276:immunology*; D008223:Lymphoma / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C
D000911:Antibodies, Monoclonal; D007143:Immunoglobulin Heavy Chains; D007075:Immunoglobulin M