Chemoattraction enrichment of thymus-homing bone marrow cells.
It has been reported that a population of cells from mouse bone marrow migrates to supernatant made from the incubation of minced fragments of new-born mouse thymuses and that the migrated population is enriched for immature lymphoid cells. In the present study, we show that this method enriches for thymic-homing cells. Migration-enriched cells were labelled with fluorescein isothiocyanate (FITC) and were injected into the tail veins of lethally irradiated mice. Cell suspensions of the thymuses from these experimental mice had 8.1 +/- 1.8% fluorescing cells compared to control mice given equal numbers of non-migration-enriched FITC labelled cells which had 2.4 +/- 1.7% positive cells.
D000818:Animals; D001854:Bone Marrow Cells; D002465:Cell Movement; D002633:Chemotaxis; D005434:Flow Cytometry; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D005456:Fluorescent Dyes; D051379:Mice; D008808:Mice, Inbred CBA; D013861:Thiocyanates; D013950:Thymus Gland / Q000166:cytology*
D005452:Fluoresceins; D005456:Fluorescent Dyes; D013861:Thiocyanates; D016650:Fluorescein-5-isothiocyanate