title: The human CK gene segment and the kappa deleting element are closely linked.
abstract: The element which mediates the deletion of the CK gene segment (abbreviations ref. 1) in human lambda light chain producing B-cells was found to be located 24 kb downstream of CK. The kappa deleting element is flanked by hepta- and nonanucleotide recognition sequences similar to the ones adjacent to the JK gene segments. Complementary recognition sequences with a 30 bp spacer were found in the JK-CK intron. For the first time the two partners of a recombination event in a mammalian immunoglobulin gene system have been linked on a physical map. The orientation of the recombination signals of the intron and the Kde sequences allows a joining by a simple deletion mechanism. Similarities and possible differences to the mechanism of V-J joining are discussed.
mesh_terms: D002460:Cell Line; D002872:Chromosome Deletion; D002874:Chromosome Mapping; D005786:Gene Expression Regulation; D008040:Genetic Linkage; D006801:Humans; D007127:Immunoglobulin Constant Regions / Q000235:genetics*; D007145:Immunoglobulin kappa-Chains / Q000235:genetics*; D007146:Immunoglobulin lambda-Chains / Q000235:genetics; D007136:Immunoglobulins / Q000235:genetics*; D011995:Recombination, Genetic
chemical_list: D007127:Immunoglobulin Constant Regions; D007145:Immunoglobulin kappa-Chains; D007146:Immunoglobulin lambda-Chains; D007136:Immunoglobulins