title: Sickle cell vaso-occlusion in an animal model; intravital microscopy and radionuclide imaging of selective sequestration of dense cells.
abstract: The impaired deformability and heterogeneity of erythrocytes in sickle cell disease endows them with abnormal microvascular flow dynamics. In the sickle cell exchange-transfused rat model, sickle cells exhibit lower volumetric flux and shear rates compared to normal (HbAA) or autologous rat cells. A subpopulation of dense sickle cells and irreversibly sickled cells show a propensity to induce occlusion at precapillary arterioles, residing at such sites for several seconds and causing local rheological disequilibrium. Radionuclide studies with indium-111 demonstrate preferential uptake of labeled HbSS cells in pulmonary microcirculation. These data are relevant to the factors that are involved in the initiation and propagatin of vaso-occlusion resulting in derangement of homeostasis in certain microvascular beds and perhaps painful crisis and selective organ injury.
mesh_terms: D000755:Anemia, Sickle Cell / Q000150:complications* / Q000000981:diagnostic imaging; D000818:Animals; D001783:Blood Flow Velocity; D004195:Disease Models, Animal; D004912:Erythrocytes / Q000502:physiology; D007205:Indium Radioisotopes; D007238:Infarction / Q000000981:diagnostic imaging / Q000209:etiology*; D008297:Male; D008643:Mesentery / Q000098:blood supply; D008833:Microcirculation / Q000000981:diagnostic imaging; D009942:Organometallic Compounds; D015125:Oxyquinoline / Q000031:analogs & derivatives; D011877:Radionuclide Imaging; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D007205:Indium Radioisotopes; D009942:Organometallic Compounds; C032950:indium oxine; D015125:Oxyquinoline