PA-II, the L-fucose and D-mannose binding lectin of Pseudomonas aeruginosa stimulates human peripheral lymphocytes and murine splenocytes.
Pseudomonas aeruginosa lectin PA-II agglutinates human peripheral lymphocytes and stimulates mitogenesis (predominantly in T cells), like the plant lectins PHA and Con A. Murine splenocytes are also agglutinated and stimulated by PA-II as by Con A. Sialidase treatment of the human and murine cells enhances their agglutination and augments the stimulation of human lymphocytes at low PA-II concentrations. The PA-II agglutinating and mitogenic effects are specifically inhibited by L-fucose. The bacterial source and the specificity of PA-II for L-fucose are both rare features among the hitherto described mitogenic lectins. However, since this lectin also binds mannose, a mannose-bearing receptor might be involved in its mitogenicity.
D018829:Adhesins, Bacterial; D000818:Animals; D001426:Bacterial Proteins / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D003208:Concanavalin A / Q000494:pharmacology; D005643:Fucose / Q000494:pharmacology; D006801:Humans; D037102:Lectins; D008213:Lymphocyte Activation / Q000187:drug effects*; D008214:Lymphocytes / Q000187:drug effects*; D008358:Mannose / Q000378:metabolism; D051379:Mice; D009439:Neuraminidase / Q000494:pharmacology; D010835:Phytohemagglutinins / Q000494:pharmacology; D011550:Pseudomonas aeruginosa; D013154:Spleen / Q000166:cytology
D018829:Adhesins, Bacterial; D001426:Bacterial Proteins; D037102:Lectins; D010835:Phytohemagglutinins; C038692:adhesin, Pseudomonas; D003208:Concanavalin A; D005643:Fucose; D009439:Neuraminidase; D008358:Mannose