An enzyme immunoassay for osteocalcin.
A sensitive and specific enzyme immunoassay for bovine osteocalcin was developed with the use of enzyme-labeled antigen. Bovine osteocalcin was conjugated with beta-D-galactosidase for use in the immunoassay. The minimum amount of osteocalcin detectable by this method was 5-10 pg/assay. The enzyme immunoassay could detect immunoreactive osteocalcin levels in normal human sera, giving a value of 6.58 +/- 0.72 ng/ml (mean +/- SE of 16 human subjects). The correlation coefficient between the levels measured by this method and radioimmunoassay was 0.94.
D000818:Animals; D002135:Calcium-Binding Proteins / Q000032:analysis* / Q000276:immunology; D002417:Cattle; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D015675:Osteocalcin; D011817:Rabbits; D011863:Radioimmunoassay; D001616:beta-Galactosidase
D002135:Calcium-Binding Proteins; D015675:Osteocalcin; D001616:beta-Galactosidase