Selective localization of gamma-enolase in stromal cells of cerebellar hemangioblastomas.
Three cases of cerebellar hemangioblastoma were studied using the immunoperoxidase technique to localize gamma-enolase, also known as neuron-specific enolase. The stromal cells demonstrated positive staining for gamma-enolase, while endothelial cells and pericytes showed no reactivity. Two vascular lesions, an angiosarcoma and a cutaneous angioma, were studied and found to be nonreactive for gamma-enolase. All tumors were also tested for factor VIII/von Willebrand factor, glial fibrillary acidic protein, and the S-100 protein. The lack of expression of gamma-enolase in endothelial cells of hemangioblastomas demonstrates a clear antigenic distinction from neighboring gamma-enolase-positive stromal cells. The significance of this finding and its implications for stromal cell histogenesis are discussed.
D000328:Adult; D002528:Cerebellar Neoplasms / Q000032:analysis / Q000201:enzymology* / Q000473:pathology; D005169:Factor VIII / Q000032:analysis; D005260:Female; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D006394:Hemangiosarcoma / Q000032:analysis / Q000201:enzymology* / Q000473:pathology; D006801:Humans; D008297:Male; D008875:Middle Aged; D010751:Phosphopyruvate Hydratase / Q000032:analysis*; D009418:S100 Proteins / Q000032:analysis
D005904:Glial Fibrillary Acidic Protein; D009418:S100 Proteins; D005169:Factor VIII; D010751:Phosphopyruvate Hydratase