Corticoids modulate FSH action in cultured porcine granulosa cells.
The effect of corticosteroids on FSH-stimulated production of progesterone and on LH/hCG receptor binding of 125I-hCG were studied in immature porcine granulosa cells. Deoxycorticosterone, deoxycorticosterone-acetate (DOC), corticosterone, aldosterone and dexamethasone enhanced FSH stimulated progesterone production in a dose-dependent manner. In contrast, cortisol cortisone and tetrahydrocorticosterone did not affect progesterone production. delta 4-androstenedione did not alter the enhancing effect of DOC. Incubation of granulosa cells with dbcAMP caused no significant increase of progesterone synthesis. All corticosteroids tested markedly inhibited stimulation of 125I-hCG binding on granulosa cells by FSH. It was concluded that the modulatory effect of corticosteroids on ovarian responsiveness to FSH consists of direct action on granulosa cells.
D000305:Adrenal Cortex Hormones / Q000494:pharmacology*; D000450:Aldosterone / Q000494:pharmacology; D000818:Animals; D002478:Cells, Cultured; D006063:Chorionic Gonadotropin / Q000378:metabolism; D003345:Corticosterone / Q000494:pharmacology; D003900:Desoxycorticosterone / Q000494:pharmacology; D003907:Dexamethasone / Q000494:pharmacology; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology*; D006107:Granulosa Cells / Q000187:drug effects / Q000378:metabolism*; D011374:Progesterone / Q000096:biosynthesis; D011974:Receptors, LH / Q000378:metabolism; D013552:Swine
D000305:Adrenal Cortex Hormones; D006063:Chorionic Gonadotropin; D011974:Receptors, LH; D003900:Desoxycorticosterone; D000450:Aldosterone; D011374:Progesterone; D003907:Dexamethasone; D005640:Follicle Stimulating Hormone; D003345:Corticosterone