title: Identification and characterization of the tyrosine protein kinases of rat spleen.
abstract: High levels of tyrosine protein kinase have been recently detected in the membranes of rat spleen. In the present report the tyrosine protein kinase activity of the 30,000 x g pellet of rat spleen has been solubilized and partially purified by ion exchange and gel permeation chromatography. Two peaks of tyrosine protein kinase of Mr 35,000 (TPK-I) and Mr 40,000 (TPK-II) have been resolved. These kinases were free of the EGF receptor and insulin receptor tyrosine protein kinases. Although TPK-I and TPK-II phosphorylated angiotensin II, casein, histone, tubulin, phosphorylase b, and p36 they differed from each other in preference for the substrates. Both tyrosine protein kinases did not phosphorylate anti-pp60v-src IgG.
mesh_terms: D000804:Angiotensin II / Q000378:metabolism; D000818:Animals; D002364:Caseins / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D066246:ErbB Receptors / Q000378:metabolism; D006657:Histones / Q000378:metabolism; D007527:Isoenzymes / Q000378:metabolism; D008970:Molecular Weight; D015688:Oncogene Protein pp60(v-src); D010763:Phosphorylase b / Q000378:metabolism; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D051381:Rats; D012191:Retroviridae Proteins / Q000378:metabolism; D013154:Spleen / Q000201:enzymology*; D013379:Substrate Specificity; D014404:Tubulin / Q000378:metabolism
chemical_list: D002364:Caseins; D006657:Histones; D007527:Isoenzymes; D012191:Retroviridae Proteins; D014404:Tubulin; D000804:Angiotensin II; D010763:Phosphorylase b; D066246:ErbB Receptors; D011505:Protein-Tyrosine Kinases; D015688:Oncogene Protein pp60(v-src)