Evidence for the existence of an Ns-type regulatory protein in Trypanosoma cruzi membranes.
The existence of a GTP-binding protein of the Ns type in Trypanosoma cruzi was explored. Epimastigote membranes were labelled by cholera toxin in the presence of [adenine-14C]NAD+. After SDS/polyacrylamide-gel electrophoresis of extracted membrane proteins, a single labelled polypeptide band of apparent Mr approx. 45,000 was detected. Epimastigote cells were treated with N-ethylmaleimide and electrofused to lymphoma S49 cells lacking the Ns protein. Evidence indicates that in such electrofusion-generated cell hybrids a heterologous adenylate cyclase system was reconstituted with the Ns protein provided by T. cruzi epimastigotes.
D000262:Adenylyl Cyclases / Q000378:metabolism; D002459:Cell Fusion; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D002772:Cholera Toxin / Q000494:pharmacology; D004560:Electricity; D019204:GTP-Binding Proteins / Q000378:metabolism*; D006822:Hybrid Cells / Q000378:metabolism; D008223:Lymphoma / Q000378:metabolism; D014349:Trypanosoma cruzi / Q000187:drug effects / Q000378:metabolism*
D002772:Cholera Toxin; D019204:GTP-Binding Proteins; D000262:Adenylyl Cyclases