Lipoprotein interaction with artery wall derived proteoglycan: comparisons between atherosclerosis-susceptible WC-2 and resistant Show Racer pigeons.
The binding of intact, high molecular weight, aortic proteoglycan (PG) isolated from grossly normal appearing aortas of atherosclerosis-susceptible White Carneau pigeons (WC-2) and -resistant Show Racer pigeons (SR) to homologous and heterologous serum lipoproteins from both normolipemic and hyperlipemic pigeons was examined. In vitro binding studies were done using a mixture of purified chondroitin sulfate PG and dermatan sulfate PG monomers to simulate an in situ composition. For each animal, a binding potential or reactivity number was calculated and corresponded to the shape and slope of the PG-LDL binding curve, where higher values indicated greater reactivity. For WC-2 normal sera, mean values were 0.97 and 0.95 using WC-2 and SR PG respectively, compared to SR normal sera (equivalent total plasma cholesterol) where values were 0.80 and 0.82. Corresponding mean reactivity values for hyperlipemic sera (diluted to a cholesterol concentration of 300 mg/dl) were 0.95, 1.00, 0.73, and 0.79. The results suggest that LDL from both normolipemic and hyperlipemic atherosclerosis-susceptible WC-2 pigeons is more reactive in complexing to artery wall derived PG than LDL from SR pigeons, regardless of PG source.
D000818:Animals; D001013:Aorta, Thoracic / Q000032:analysis* / Q000503:physiopathology; D001161:Arteriosclerosis / Q000209:etiology / Q000503:physiopathology*; D002809:Chondroitin Sulfates / Q000378:metabolism; D010856:Columbidae / Q000235:genetics* / Q000276:immunology; D004198:Disease Susceptibility; D004587:Electrophoresis, Agar Gel; D006949:Hyperlipidemias / Q000097:blood; D007113:Immunity, Innate; D008074:Lipoproteins / Q000378:metabolism*; D008077:Lipoproteins, LDL / Q000378:metabolism; D011485:Protein Binding; D011487:Protein Conformation; D011509:Proteoglycans / Q000302:isolation & purification / Q000378:metabolism*
D008074:Lipoproteins; D008077:Lipoproteins, LDL; D011509:Proteoglycans; D002809:Chondroitin Sulfates