[Determination of alkylation of bacterial DNA as a rapid test for toxicological evaluation of alkylating xenobiotic agents].
Alkylated purine bases from hydrolized DNA can be separated by HPLC and quantified with a fluorescence detector. We applied this method to bacterial DNA. 7-methylguanine was detected after treatment of Serratia marcescens with iodoacetamide, dimethyl sulfate and with polluted air.
D000393:Air Pollutants / Q000032:analysis; D000477:Alkylating Agents / Q000032:analysis* / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D004269:DNA, Bacterial / Q000032:analysis* / Q000302:isolation & purification; D006147:Guanine / Q000031:analogs & derivatives / Q000032:analysis; D006847:Hydrocarbons, Iodinated / Q000494:pharmacology; D007460:Iodoacetamide / Q000494:pharmacology; D012706:Serratia marcescens / Q000187:drug effects / Q000235:genetics*; D013050:Spectrometry, Fluorescence; D013463:Sulfuric Acid Esters / Q000494:pharmacology
D000393:Air Pollutants; D000477:Alkylating Agents; D004269:DNA, Bacterial; D006847:Hydrocarbons, Iodinated; D013463:Sulfuric Acid Esters; D006147:Guanine; C008450:7-methylguanine; C014055:methyl iodide; C007482:dimethyl sulfate; D007460:Iodoacetamide
Bestimmung der Alkylierung bakterieller DNS als Schnelltest zur toxikologischen Beurteilung von alkylierend wirkenden Xenobiotika.