Bilirubin conjugate excretion and bilirubin uridine diphosphoglucuronyltransferase activity in nonjaundiced homozygous and heterozygous Gunn rats.
High-performance liquid chromatography was used to analyze the composition of bilirubin conjugates in bile from adult male and female outbred normal (JJ) and heterozygous (Jj) Gunn rats. Bile was examined directly without prior derivatization or extraction. Significantly higher bilirubin diglucuronide and lower bilirubin monoglucuronide (both C-8 and C-12 isomers) excretion in JJ rats was demonstrated. Bilirubin monoglucuronide C-8/C-12 ratios were similar in both genotypes, as was the percentage of bilirubin monoglucuronide monoglycoside diester. Hepatic bilirubin uridine diphosphoglucuronyltransferase activity showed a statistically significant positive correlation with the relative amount of bilirubin diglucuronide present in bile and a significant negative correlation with the amount of bilirubin monoglucuronide. The relative percentage of bilirubin monoglucuronide vs. diglucuronide in bile allows an indirect assessment of hepatic bilirubin uridine diphosphoglucuronyltransferase activity.
D000818:Animals; D001663:Bilirubin / Q000378:metabolism*; D005260:Female; D005965:Glucuronates / Q000378:metabolism; D014453:Glucuronosyltransferase / Q000378:metabolism*; D006579:Heterozygote; D006720:Homozygote; D008099:Liver / Q000201:enzymology; D008297:Male; D051381:Rats; D011911:Rats, Gunn
D005965:Glucuronates; D014453:Glucuronosyltransferase; D001663:Bilirubin