Transformation in cyanobacteria.
The lack of any known transduction or indigenous conjugation systems has left transformation as the major means for genetic manipulations in cyanobacteria. Studies of transformation in cyanobacteria generally have dealt with one of two distinct areas. The first area is genomic transformation where internalized donor DNA recombines with chromosomally located genes. Chromosomal transformation can be a powerful tool for genetic mapping and mutagenesis. The second area is plasmid transformation where internalized plasmid donor DNA becomes established as an independent replicon in the recipient cyanobacterium. This second area has received a great deal of attention because it allows the generation of merodiploids for studies of genetic regulation and control and because it potentially allows the expression of foreign genes in an oxygenic photoautotroph. This article will attempt to describe the development of our current understanding of these two types of genetic transformation in cyanobacteria.
D002874:Chromosome Mapping; D000458:Cyanobacteria / Q000235:genetics*; D004247:DNA / Q000235:genetics*; D005796:Genes; D010957:Plasmids; D014170:Transformation, Genetic
D004247:DNA