Benzo(a)pyrene hydroxylase activity in human bronchial mucus.
Sputum collected from patients with respiratory diseases were examined for presence of benzo(a)pyrene hydroxylase (BPH) activity. The human bronchial mucus used in these studies had significant capability to metabolize benzo(a)pyrene. Clarification of the sputum by agents such as N-acetylcysteine or pancreatin in presence of antibiotics was found to be essential for the detection of BPH activity. In vitro incubation of the clarified human bronchial mucus with benzoflavone caused inhibition, while 7,8-dimethyl-benzanthracene induced BPH enzyme activity.
D015127:9,10-Dimethyl-1,2-benzanthracene / Q000494:pharmacology; D001189:Aryl Hydrocarbon Hydroxylases / Q000378:metabolism*; D001571:Benzoflavones / Q000494:pharmacology; D001579:Benzopyrene Hydroxylase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D001980:Bronchi; D004789:Enzyme Activation / Q000187:drug effects; D006801:Humans; D009093:Mucus / Q000201:enzymology*; D013183:Sputum / Q000201:enzymology
D001571:Benzoflavones; D015127:9,10-Dimethyl-1,2-benzanthracene; C011512:alpha-naphthoflavone; D001579:Benzopyrene Hydroxylase; D001189:Aryl Hydrocarbon Hydroxylases