Intracellular distribution of 73,000 and 72,000 dalton heat shock proteins in HeLa cells.
Intracellular localization of 73,000 and 72,000 dalton heat shock proteins (HSP73/72) in HeLa cells that were heat shocked or treated with chemical stressors was investigated using indirect immunofluorescent staining. The antiserum used specifically recognized the HSP73/72 in HeLa cells, and HSPs were increased by heating cells at 42 degrees C for 2 or 4 h and by prior treatment with chemical stressors (sodium arsenite, cadmium chloride, 8-hydroxyquinoline and ethanol). There was diffuse cytoplasmic staining at 37 degrees C, whereas nucleoli were stained brightly when cells were heated at 42 degrees C for 2 h. This rapid accumulation of HSP73/72 in the nucleoli was not inhibited by cycloheximide (50 micrograms/ml). Translocation of HSPs to the nucleoli was specific for heat because no translocation was induced by treatment with chemical stressors. When the cells were returned to 37 degrees C after heating, the HSPs in their nucleoli disappeared rapidly and diffuse cytoplasmic staining was present after 6-9 h. Our results suggest that the transient accumulation of HSP73/72 in HeLa cell nucleoli that is induced by heat shock is not correlated with the development of thermotolerance obtained in other cell systems.
D000818:Animals; D001151:Arsenic / Q000494:pharmacology; D018053:Arsenites; D002104:Cadmium / Q000494:pharmacology; D019256:Cadmium Chloride; D002466:Cell Nucleolus / Q000648:ultrastructure; D000431:Ethanol / Q000494:pharmacology; D005455:Fluorescent Antibody Technique; D006367:HeLa Cells / Q000648:ultrastructure*; D006360:Heat-Shock Proteins / Q000096:biosynthesis*; D006801:Humans; D007124:Immunoenzyme Techniques; D007158:Immunologic Techniques; D015125:Oxyquinoline / Q000494:pharmacology; D011817:Rabbits; D017670:Sodium Compounds; D013997:Time Factors
D018053:Arsenites; D006360:Heat-Shock Proteins; D017670:Sodium Compounds; D002104:Cadmium; D000431:Ethanol; C017947:sodium arsenite; D015125:Oxyquinoline; D019256:Cadmium Chloride; D001151:Arsenic