Neuronal and glial proteins in medulloblastomas. II. Heterotransplantation of human medulloblastoma.
Seven resected medulloblastomas were inoculated into hereditary athymic (nude) and asplenic athymic (Lasat) mice. From these, only one transplantable tumor line was obtained, in nude mice. We studied the transplanted medulloblastoma immunohistochemically and by electron microscope. In addition, using enzyme immunoassay, the gamma-enolase contents of the transplanted medulloblastomas were measured and the results were compared with those of transplanted glioblastomas. Neurofilament proteins (NF68Kd, NF160Kd) and gamma-enolase positive cells were maintained in the successful transplants of medulloblastomas, but the GFAP positive cells were lost. Under the electron microscope, a few cells with ten nanometer intermediate filaments and microtubules were observed in the transplanted tumor. The medulloblastoma possesses some of the neuronal characteristics which are maintained in the transplants in nude mice.
D000818:Animals; D002528:Cerebellar Neoplasms / Q000032:analysis*; D002675:Child, Preschool; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis*; D005910:Glioma / Q000032:analysis; D006801:Humans; D007124:Immunoenzyme Techniques; D007381:Intermediate Filament Proteins / Q000032:analysis*; D008297:Male; D008527:Medulloblastoma / Q000032:analysis*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008854:Microscopy, Electron; D009368:Neoplasm Transplantation; D016900:Neurofilament Proteins; D010751:Phosphopyruvate Hydratase / Q000032:analysis*
D005904:Glial Fibrillary Acidic Protein; D007381:Intermediate Filament Proteins; D016900:Neurofilament Proteins; D010751:Phosphopyruvate Hydratase