Application of flow cytometry to immunohematology.
A method is described for applying flow cytometry to the analysis of populations of red blood cells that have been sensitized, in vivo or in vitro, with IgG antibody. Specific instrument settings and details of the method are given with sufficient explanation for practical use in immunohematologic investigations.
D000937:Antigen-Antibody Reactions; D000954:Antigens, Surface / Q000032:analysis; D004912:Erythrocytes / Q000145:classification / Q000166:cytology* / Q000276:immunology; D005434:Flow Cytometry / Q000379:methods*; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins / Q000494:pharmacology; D006402:Hematologic Diseases / Q000276:immunology*; D006801:Humans; D007070:Immunoglobulin A / Q000378:metabolism; D007074:Immunoglobulin G / Q000378:metabolism; D007075:Immunoglobulin M / Q000378:metabolism; D007834:Lasers; D011485:Protein Binding; D013861:Thiocyanates / Q000494:pharmacology
D000954:Antigens, Surface; D005452:Fluoresceins; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D013861:Thiocyanates; D016650:Fluorescein-5-isothiocyanate