title: Responses of the L5178Y tk+/tk- mouse lymphoma cell forward mutation assay to coded chemicals. I: Results for nine compounds.
abstract: Nine substances were tested for their mutagenic potential in the L5178Y tk+/tk- mouse lymphoma cell forward mutation assay, by means of procedures based upon those described by Clive and Spector (Mutat Res 44:269-278, 1975) and Clive et al (Mutat Res 59:61-108, 1979). Cultures were exposed to the chemicals for 4 hr, then cultured for 2 days before plating in soft agar with or without trifluorothymidine (TFT), 3 micrograms/ml. The coded chemicals were tested at least twice. Significant responses were obtained with calcium chromate, 3-(chloromethyl)pyridine, 1,2-epoxybutane, monochloroacetic acid, dicyclohexylthiourea, 2,4-diaminophenol hydrochloride, and pentachloroanisole. Apart from pentachloroanisole, rat liver S9 mix was not a requirement for the clearly mutagenic activity of any of these compounds. Compounds not identified as mutagens were 3-amino-1,2,4-triazole and sucrose.
mesh_terms: D000085:Acetates / Q000633:toxicity; D000627:Aminophenols / Q000633:toxicity; D000640:Amitrole / Q000633:toxicity; D000818:Animals; D000840:Anisoles / Q000633:toxicity; D001711:Biotransformation; D017610:Calcium Compounds; D002273:Carcinogens; D002460:Cell Line; D002840:Chromates / Q000633:toxicity; D004852:Epoxy Compounds / Q000633:toxicity; D008223:Lymphoma; D051379:Mice; D008862:Microsomes, Liver / Q000378:metabolism; D009152:Mutagenicity Tests / Q000592:standards*; D009153:Mutagens; D011725:Pyridines / Q000633:toxicity; D013395:Sucrose / Q000633:toxicity; D013890:Thiourea / Q000031:analogs & derivatives / Q000633:toxicity; D013937:Thymidine Kinase / Q000235:genetics
chemical_list: D000085:Acetates; D000627:Aminophenols; D000840:Anisoles; D017610:Calcium Compounds; D002273:Carcinogens; D002840:Chromates; D004852:Epoxy Compounds; D009153:Mutagens; D011725:Pyridines; C027488:1,2-epoxybutane; C015421:pentachloroanisole; D013395:Sucrose; C006972:chloroacetic acid; C016375:N,N'-dicyclohexylthiourea; C051822:3-(chloromethyl)pyridine; D013937:Thymidine Kinase; D013890:Thiourea; C034362:2,4-diaminophenol; C033750:calcium chromate(VI); C023666:pyridine; D000640:Amitrole