Amino acid sequence determination of guanyl-specific ribonuclease Sa from Streptomyces aureofaciens.
Using automated Edman degradation of two nonfractionated peptide mixtures of tryptic and staphylococcal protease digests of the protein, the complete amino acid sequence of the guanyl-specific ribonuclease Sa from Streptomyces aureofaciens was established. Ribonuclease Sa contains 96 amino acid residues (Mr 10,566). A 50% sequence homology of ribonuclease Sa to the guanyl-specific ribonuclease St from S. erythreus was found.
D000595:Amino Acid Sequence; D010450:Endopeptidases; D004722:Endoribonucleases / Q000302:isolation & purification; D008666:Metalloendopeptidases; D010446:Peptide Fragments / Q000032:analysis; D006163:Ribonuclease T1 / Q000302:isolation & purification; D013304:Streptomyces aureofaciens / Q000201:enzymology*; D014357:Trypsin
D010446:Peptide Fragments; D004722:Endoribonucleases; D006163:Ribonuclease T1; D010450:Endopeptidases; D014357:Trypsin; D008666:Metalloendopeptidases; C022653:auR protein, Staphylococcus aureus