title: Amino acid sequence determination of guanyl-specific ribonuclease Sa from Streptomyces aureofaciens.
abstract: Using automated Edman degradation of two nonfractionated peptide mixtures of tryptic and staphylococcal protease digests of the protein, the complete amino acid sequence of the guanyl-specific ribonuclease Sa from Streptomyces aureofaciens was established. Ribonuclease Sa contains 96 amino acid residues (Mr 10,566). A 50% sequence homology of ribonuclease Sa to the guanyl-specific ribonuclease St from S. erythreus was found.
mesh_terms: D000595:Amino Acid Sequence; D010450:Endopeptidases; D004722:Endoribonucleases / Q000302:isolation & purification; D008666:Metalloendopeptidases; D010446:Peptide Fragments / Q000032:analysis; D006163:Ribonuclease T1 / Q000302:isolation & purification; D013304:Streptomyces aureofaciens / Q000201:enzymology*; D014357:Trypsin
chemical_list: D010446:Peptide Fragments; D004722:Endoribonucleases; D006163:Ribonuclease T1; D010450:Endopeptidases; D014357:Trypsin; D008666:Metalloendopeptidases; C022653:auR protein, Staphylococcus aureus