Novel method for detecting micro-organisms in blood cultures.
A method for detecting the growth of micro-organisms in blood culture by a visual signal is described. The system utilises a single blood culture medium that has been specifically formulated to support growth of aerobic, anaerobic, and microaerophilic micro-organisms. The system is based on the principle that when micro-organisms grow in the medium in a sealed bottle their metabolic products create positive pressure. This positive pressure displaces the infected blood and broth into an upper chamber, which acts as a visual signal of microbial activity. All the test micro-organisms, when inoculated at less than 20 colony forming units into simulated human blood cultures, gave a positive signal.
D001769:Blood / Q000382:microbiology*; D002245:Carbon Dioxide / Q000032:analysis; D003470:Culture Media; D006801:Humans; D008828:Microbiological Techniques; D009584:Nitrogen / Q000032:analysis; D010100:Oxygen / Q000032:analysis; D013997:Time Factors
D003470:Culture Media; D002245:Carbon Dioxide; D009584:Nitrogen; D010100:Oxygen