Characterization of kininogen deficiency of Brown Norway rat mutant Katholiek strain.
A deficiency in the plasma kallikrein-kinin system of Brown Norway rat mutant Katholiek strain (B/N-Ka), first reported by Damas et al. was further characterized. The prolonged activated partial thromboplastin time (APTT) of B/N-Ka rat plasma was corrected by an addition of rat HMW-kininogen, indicating B/N-Ka rat is deficient in HMW kininogen. The study of kinin-release of plasmas of the three strains of rat (B/N-Ka, B/N Ki and SD) by several kininogenases expressed that B/N-Ka rat is deficient in LMW kininogen, in addition to HMW kininogen deficiency. The plasmas of the three strains of rat were gel-filtered through Sephacryl S-200 gel and profiles of kinin-release of the fractions were examined by several kininogenases. The result demonstrated that normal rat plasma contains three kinds of kininogen (HMW and LMW kininogens, and T-kininogen), and B/N-Ka plasma contains only T-kininogen. B/N-Ka rat plasma demonstrated T-kininogen antigen but no HMW kininogen by the study of immunodiffusion using their antisera raised in rabbits.
D000818:Animals; D005260:Female; D006168:Guinea Pigs; D005779:Immunodiffusion; D007700:Kinetics; D007704:Kininogens / Q000097:blood / Q000235:genetics*; D007705:Kinins / Q000097:blood; D008297:Male; D009154:Mutation; D051381:Rats; D011919:Rats, Inbred Strains; D011922:Rats, Mutant Strains; D013045:Species Specificity
D007704:Kininogens; D007705:Kinins