Abnormal B-cell function in hemophiliacs treated with cryoprecipitate and factor VIII and IX concentrates.
B-Cell function was evaluated in a group of 43 patients with factor VIII or factor IX deficiency. Thirty had been treated primarily with cryoprecipitate and 13 with concentrates of factor VIII or IX. Serum immunoglobulin G levels were found to be diffusely elevated; however, the absolute number of mature B cells in peripheral blood was normal. B-Cell function as measured by testing mitogen-induced proliferation and and immunoglobulin secretion by plaque-forming cells (PFC) in vitro was reduced. Coculture experiments suggested that these abnormal B-cell responses might be secondary to increased suppressor T-cell activity, which was found more frequently in patients seropositive for antibody to lymphadenopathy-associated virus. Both seronegative and seropositive patients had reduced responses in the proliferative and PFC assays, but the lowest PFC responses occurred in the seropositive group.
D000293:Adolescent; D000328:Adult; D000368:Aged; D001402:B-Lymphocytes / Q000276:immunology*; D002648:Child; D005164:Factor IX / Q000627:therapeutic use*; D005169:Factor VIII / Q000627:therapeutic use*; D005340:Fibrinogen / Q000627:therapeutic use*; D006462:Hemolytic Plaque Technique; D006467:Hemophilia A / Q000188:drug therapy / Q000276:immunology*; D002836:Hemophilia B / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D008875:Middle Aged
D007074:Immunoglobulin G; C026912:cryoprecipitate coagulum; D005169:Factor VIII; D005164:Factor IX; D005340:Fibrinogen