Cytogenetic analysis in workers occupationally exposed to nickel carbonyl.
Chromosomal aberration and sister-chromatid exchange (SCE) base-line frequencies and SCE frequencies induced by 10 ng/ml mitomycin C (MMC) were analysed in cultured peripheral lymphocytes of 65 workers occupationally exposed to nickel carbonyl Ni(CO)4. The subjects were divided into 4 groups: (1) control; (2) exposed to nickel carbonyl (= exposed); (3) cigarette smokers; (4) smoking-exposed. The results show that there are no significant differences in chromosomal aberration frequencies, breaks or gaps, between the various groups. However, the SCE base-line frequency of the smoking-exposed group, with an average of 7.7/cell, was significantly higher than that of the control group, with an average of 6.5/cell (P less than 0.01), and also than that of the exposed group with an average of 5.9/cell (P less than 0.01). Similarly, the SCE frequency induced by 10 ng/ml MMC in the smoking-exposed group which averaged 15.5/cell was significantly higher than that of the control group (average of 13.2/cell (P less than 0.05], and also than that of the exposed group with an average of 12.3/cell (P less than 0.01). Under our experimental conditions, it may be that the level of exposure was not high enough to elicit an increase in chromosomal aberrations and SCE frequencies in the non-smoker exposed group. The fact that an increase in SCE frequencies was only found in the smoking-exposed group implies that the two factors, smoking and exposure to nickel carbonyl, are jointly responsible for the result.
D002478:Cells, Cultured; D002869:Chromosome Aberrations; D003043:Cocarcinogenesis; D004781:Environmental Exposure; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects / Q000648:ultrastructure; D008669:Metallurgy; D016685:Mitomycin; D008937:Mitomycins / Q000494:pharmacology; D009152:Mutagenicity Tests; D009790:Occupations; D009942:Organometallic Compounds / Q000494:pharmacology*; D012854:Sister Chromatid Exchange / Q000187:drug effects*; D012907:Smoking
D008937:Mitomycins; D009942:Organometallic Compounds; C005303:nickel carbonyl; D016685:Mitomycin