A monoclonal anti-mouse LFA-1 alpha antibody mimics the biological effects of B cell stimulatory factor-1 (BSF-1).
This study describes the generation of a monoclonal mouse x rat antibody (G-48) that recognizes a determinant on the serologically defined LFA-1 alpha-chain. It immunoprecipitates two noncovalently associated polypeptides of 176,000 and 95,000 Mr respectively from lysates of radioiodinated BCL1 cells, T cells, and B cells. G-48 mimics the biological actions of BSF-1 by inducing increased levels of Ia antigen expression on resting B cells, augmenting the proliferation of anti-delta-stimulated B cells, and in insolubilized form, inducing IgG1 secretion by LPS-activated B cells. G-48 does not have BCDF mu, BCGF II, nor IL 2 activity. These results demonstrate that LFA-1 plays an important role in B cell activation, proliferation, and differentiation.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology / Q000502:physiology*; D001402:B-Lymphocytes / Q000187:drug effects* / Q000276:immunology; D002454:Cell Differentiation / Q000187:drug effects; D005260:Female; D006133:Growth Substances / Q000494:pharmacology*; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D007138:Immunoglobulin delta-Chains / Q000276:immunology; D015847:Interleukin-4; D008213:Lymphocyte Activation / Q000187:drug effects; D016169:Lymphocyte Function-Associated Antigen-1; D008222:Lymphokines / Q000494:pharmacology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D019948:Receptors, Interleukin-4; D011975:Receptors, Mitogen / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D006133:Growth Substances; D000949:Histocompatibility Antigens Class II; D007138:Immunoglobulin delta-Chains; D016169:Lymphocyte Function-Associated Antigen-1; D008222:Lymphokines; D019948:Receptors, Interleukin-4; D011975:Receptors, Mitogen; D015847:Interleukin-4