Effects of 4-nitroquinoline 1-oxide on the DNA-protein complex.
The effects of 4-nitroquinoline 1-oxide (4NQO), a well known carcinogenic compound, on the DNA-protein complex and DNA Folding Proteins were investigated. FM3A cells were treated with 10(-6) M or 10(-5) M 4NQO for 30 min. Treatment with the 10(-6) M concentration was confirmed to cause the sedimentation of the DNA-protein complex to become slower. DNA Folding Proteins were then isolated from 4NQO-treated and untreated control cells and analyzed by SDS-polyacrylamide gel electrophoresis. No appreciable differences in the amounts of the major components of DNA Folding Proteins could be found due to 4NQO-treatment, but the 92 K protein was induced in DNA-protein complex by treatment with 4NQO.
D015112:4-Nitroquinoline-1-oxide / Q000494:pharmacology*; D000818:Animals; D002460:Cell Line; D004278:DNA, Superhelical / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D008325:Mammary Neoplasms, Experimental; D051379:Mice; D008809:Mice, Inbred C3H; D008970:Molecular Weight; D009600:Nitroquinolines / Q000494:pharmacology*
D004278:DNA, Superhelical; D004268:DNA-Binding Proteins; D009600:Nitroquinolines; D015112:4-Nitroquinoline-1-oxide