Estrone-3-glucuronide chemiluminescence immunoassay: an alternative method for monitoring induction of ovulation with human menopausal gonadotropin in an in vitro fertilization program.
A simple, rapid, and sensitive solid-phase immunoassay procedure for the determination of estrone-3-glucuronide (E1-3-G), which uses chemiluminescence as the end point in unextracted morning urine, is described. Thirty-one patients undergoing induction of ovulation in an in vitro fertilization (IVF) unit participated in the study. From day 3 of the menstrual cycle until the day of hCG administration, morning blood samples and morning urine specimens were collected for the determination of serum 17 beta-estradiol (E2) and urine E1-3-G, respectively. A good correlation was noted between E2 measured by radioimmunoassay (RIA) and the E1-3-G measured by chemiluminescence immunoassay (CIA), from day 5 up to the day of hCG administration (0.6 less than r less than 0.85, P less than 0.001). It is evident from this study that the CIA measurement of E1-3-G in morning urine is an accurate and rapid (2.5 hours) method and is convenient for monitoring induction of ovulation with human menopausal gonadotropins.
D000328:Adult; D006063:Chorionic Gonadotropin / Q000627:therapeutic use; D004970:Estrone / Q000031:analogs & derivatives* / Q000097:blood; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D007158:Immunologic Techniques; D008163:Luminescent Measurements; D008596:Menotropins / Q000627:therapeutic use*; D010062:Ovulation Induction
D006063:Chorionic Gonadotropin; C015856:estrone-3-glucuronide; D004970:Estrone; D008596:Menotropins