Immunochemical characterization of saline-extracted antigens of Mycobacterium tuberculosis H37Ra.
The saline extract (SE) of Mycobacterium tuberculosis H37Ra yielded four major fractions on Sephadex G-100, with average molecular weights of 48,000 (fraction I, F I) 32,000 (fraction II, F II), 15,000 (fraction III, F III) and 5,000 (fraction IV, F IV). FI and FII gave single bands on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, showed complete immunological cross-reactivity with each other and were more potent in reacting with antibodies in patients' (tuberculosis) sera than sonicate, SE, purified protein derivative, F III and F IV when tested in a competition enzyme-linked immunosorbent assay.
D000942:Antigens, Bacterial / Q000302:isolation & purification*; D002850:Chromatography, Gel; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006801:Humans; D007120:Immunochemistry; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D012698:Serologic Tests; D012965:Sodium Chloride / Q000494:pharmacology; D014376:Tuberculosis / Q000175:diagnosis; D014385:Tuberculosis, Gastrointestinal / Q000175:diagnosis / Q000276:immunology
D000942:Antigens, Bacterial; D012965:Sodium Chloride