Effects of chondroitin-4-sulphate on ultrastructure of ovarian granulosa cells.
Granulosa cells from medium sized porcine ovarian follicles exhibit inhibition of progesterone secretion after both 3 and 6 days of incubation in the presence of 2 mg chondroitin-4-sulphate (C-4-S)/ml. After 3 days of incubation some relatively small and undifferentiated granulosa cells are present in the above cultures. Six days following initiation of incubation in the presence of 2 mg C-4-S/ml many of the larger granulosa cells taken from medium sized follicles reveal lysis and/or resorption of material (probably lipoprotein) from their originally dense staining bodies as well as other indications of stress and degeneration.
D000818:Animals; D002478:Cells, Cultured; D002807:Chondroitin / Q000031:analogs & derivatives*; D002809:Chondroitin Sulfates / Q000494:pharmacology*; D005260:Female; D006107:Granulosa Cells / Q000187:drug effects / Q000648:ultrastructure*; D008854:Microscopy, Electron; D010053:Ovary / Q000166:cytology*; D011374:Progesterone / Q000378:metabolism; D013552:Swine
D011374:Progesterone; D002807:Chondroitin; D002809:Chondroitin Sulfates