Facilitated transfer of glucose from blood into perilymph in the rat cochlea.
The transport of glucose into cochlear endolymph, perilymph of scala vestibuli and perilymph of scala tympani, and cerebrospinal fluid (CSF) was studied after intravenous administration of tracers of D-glucose, L-glucose, and 3-O-methyl-D-glucose in anesthetized rats. The data showed that D-glucose concentrations in perilymph of scala vestibuli, perilymph of scala tympani, and CSF were approximately 50%, and in endolymph less than 10%, that in plasma; D-glucose concentration in perilymph of scala vestibuli, perilymph of scala tympani, and CSF increased as a linear function of that in plasma; D-glucose entry into perilymph of scala vestibuli, perilymph of scala tympani, and CSF was more rapid than that of L-glucose; after infusion of 3-O-methyl-D-glucose, but not after that of mannitol, both the D-glucose concentration ratio of perilymph over plasma and D-glucose transfer into perilymph were lowered. These results indicate that D-glucose enters into perilymph of scala vestibuli by a facilitated transport, possibly located at the blood-perilymph barrier.
D019325:3-O-Methylglucose; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism*; D003051:Cochlea / Q000378:metabolism*; D005947:Glucose / Q000134:cerebrospinal fluid / Q000378:metabolism; D007700:Kinetics; D007761:Labyrinthine Fluids / Q000378:metabolism*; D008297:Male; D008353:Mannitol / Q000494:pharmacology; D008757:Methylglucosides / Q000494:pharmacology; D009994:Osmolar Concentration; D010498:Perilymph / Q000378:metabolism*; D010693:Phloretin / Q000494:pharmacology; D010695:Phlorhizin / Q000494:pharmacology; D011188:Potassium / Q000097:blood / Q000134:cerebrospinal fluid / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012964:Sodium / Q000097:blood / Q000134:cerebrospinal fluid / Q000378:metabolism
D001786:Blood Glucose; D008757:Methylglucosides; D019325:3-O-Methylglucose; D008353:Mannitol; D012964:Sodium; D010695:Phlorhizin; D005947:Glucose; D011188:Potassium; D010693:Phloretin