Comparison of enzyme-linked immunosorbent assay and radioimmunoprecipitation test for detection of immunoglobulin A antibodies to Mycoplasma pneumoniae in nasal secretions.
An immunoglobulin A (IgA) enzyme-linked immunosorbent assay was developed and compared with the radioimmunoprecipitation test in determinations of IgA antibodies to Mycoplasma pneumoniae. Of 45 nasal secretions obtained from infected volunteers, 42 (93.4%) were positive and 2 (4.4%) were negative in both tests. The IgA enzyme-linked immunosorbent assay is at least as sensitive as the IgA radioimmunoprecipitation test but is simpler and safer and should therefore be preferred.
D000907:Antibodies, Bacterial / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007071:Immunoglobulin A, Secretory / Q000032:analysis*; D009177:Mycoplasma pneumoniae / Q000276:immunology*; D009297:Nasal Mucosa / Q000276:immunology / Q000378:metabolism*; D011019:Pneumonia, Mycoplasma / Q000276:immunology*; D011863:Radioimmunoassay
D000907:Antibodies, Bacterial; D007071:Immunoglobulin A, Secretory