EDTA-resistant activation of the terminal route of mouse complement after partial precipitation of serum with polyethylene glycol.
A new type of activation of the terminal route of complement is described. It occurs in precipitates of mouse serum prepared with a critical amount of polyethylene glycol. The activation proceeds in the presence of 10 mM EDTA and in the absence of C1q and functional C2, C3, C4 and factor B, which suggests that it is classical and alternative pathway-independent. The activation is demonstrated by the generation of both thermo-labile haemolytic and thermo-stable chemotactic activity. For both activities C5 seems to be essential. The haemolytic activity does not show species restriction towards sheep erythrocytes, which suggests a similarity with C56-initiated (reactive) but not with (S)C5-9-mediated (deviated) haemolysis. The identity of the haemolytic complex and the chemoattractant and the possible use of the new activation for the functional analysis of the mouse terminal complement route will be the subject of further study.
D000818:Animals; D011232:Chemical Precipitation; D002633:Chemotaxis; D003167:Complement Activation / Q000187:drug effects*; D003182:Complement C5 / Q000378:metabolism; D003165:Complement System Proteins / Q000302:isolation & purification / Q000378:metabolism; D004492:Edetic Acid / Q000494:pharmacology*; D006461:Hemolysis; D006358:Hot Temperature; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D011092:Polyethylene Glycols; D011817:Rabbits
D003182:Complement C5; D011092:Polyethylene Glycols; D003165:Complement System Proteins; D004492:Edetic Acid