Mutagen detection with a mouse line containing 3 distinct mutations conferring sensitivity.
A mouse-cell mutant sensitive to methyl methanesulfonate (MMS), X-rays, ultraviolet light (UV), and crosslinking agents was selected using the replica plating and cell suspension spotting methods. This mutant (XUM1) is a mitomycin C-sensitive derivative of previously reported XU1, a mutant sensitive to MMS, X-rays and UV. Since XU1 is highly susceptible to the lethal effect of 4-nitroquinoline-1-oxide (4NQO), XUM1 is also hypersensitive to 4NQO. Growth inhibition area tests showed that low concentrations of mutagens were detected with the multiple mutagen-sensitive mutant XUM1. Hence XUM1 cells will be useful in detecting with high sensitivity a wide range of mutagens and carcinogens which mimic X-rays, UV and crosslinking agents.
D015112:4-Nitroquinoline-1-oxide / Q000494:pharmacology; D000818:Animals; D002460:Cell Line / Q000187:drug effects / Q000528:radiation effects; D003432:Cross-Linking Reagents / Q000494:pharmacology; D004260:DNA Repair; D004351:Drug Resistance; D007940:Leukemia L5178 / Q000473:pathology; D008741:Methyl Methanesulfonate / Q000494:pharmacology; D051379:Mice; D016685:Mitomycin; D008937:Mitomycins / Q000494:pharmacology; D009152:Mutagenicity Tests / Q000379:methods*; D009154:Mutation; D011836:Radiation Tolerance; D014466:Ultraviolet Rays; D014965:X-Rays
D003432:Cross-Linking Reagents; D008937:Mitomycins; D016685:Mitomycin; D015112:4-Nitroquinoline-1-oxide; D008741:Methyl Methanesulfonate