Cytochalasin B changes the cytoskeletal organization in Newcastle disease virus-infected cells.
The microfilament structures of Newcastle disease virus (NDV)-infected BHK-21 cells were studied in the presence (5 micrograms/ml) or absence of cytochalasin B (CB) by means of phase contrast, indirect immunofluorescence and thin-section immunoelectron microscopy. The results indicated that CB treatment not only impaired virus infections titers and antiactin fluorescence strength but also disrupted cytoplasmic membrane and untagged ferritin-conjugated antibody on the surface of NDV specific antigens.
D000199:Actins / Q000032:analysis; D000818:Animals; D002460:Cell Line; D002472:Cell Transformation, Viral / Q000187:drug effects; D002642:Chick Embryo; D006224:Cricetinae; D003571:Cytochalasin B / Q000633:toxicity*; D003599:Cytoskeleton / Q000187:drug effects / Q000648:ultrastructure*; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes; D007668:Kidney; D008854:Microscopy, Electron; D009522:Newcastle disease virus / Q000235:genetics*; D013861:Thiocyanates
D000199:Actins; D005452:Fluoresceins; D005456:Fluorescent Dyes; D013861:Thiocyanates; D003571:Cytochalasin B; D016650:Fluorescein-5-isothiocyanate