Metabolism of sterols of varying ring unsaturation and methylation by Caenorhabditis elegans.
The metabolism of three dietary 4,4-desmethylsterols and two 4 alpha-methylsterols was investigated in the free-living nematode Caenorhabditis elegans. Dietary cholestanol was converted mostly to lathosterol. Dietary lathosterol, 7-dehydrocholesterol, 4 alpha-methylcholest-7-enol and 4 alpha-methylcholest-8(14)-enol each remained largely unchanged. An absolute requirement for a substantial quantity of 7-dehydrocholesterol in C. elegans did not exist. C. elegans was unable to remove a 4 alpha-methyl group or introduce a double bond at C-5 and also demonstrated the lack of a delta 7-reductase. Its nutritional sterol requirement was satisfied by cholestanol, lathosterol or 7-dehydrocholesterol; growth was comparable to that obtained previously in media containing delta 5-sterols. However, the two 4 alpha-methylsterols appeared to be unsatisfactory sterol nutrients. The possible physiological importance of 4 alpha-methylsterols is discussed briefly.
D000818:Animals; D002107:Caenorhabditis / Q000254:growth & development / Q000378:metabolism*; D004083:Cholestanol / Q000378:metabolism; D008055:Lipids / Q000032:analysis; D008745:Methylation; D013261:Sterols / Q000032:analysis / Q000378:metabolism*
D008055:Lipids; D013261:Sterols; D004083:Cholestanol