title: Differential hydrolysis of phospholipid molecular species during activation of human platelets with thrombin and collagen.
abstract: Mass changes in the various molecular species of phospholipids were determined after stimulation of human platelets with thrombin and collagen. Upon stimulation, every molecular species of phosphatidylinositol and phosphatidylserine was equally hydrolyzed, whereas the molecular species of phosphatidylcholine and diacyl- and alkenylacylphosphatidylethanolamine containing arachidonic acid were selectively hydrolyzed. At low Ca2+ concentrations, which result from mobilization of intracellular Ca2+ stores, phosphatidylinositol, phosphatidylcholine, and diacylphosphatidylethanolamine were hydrolyzed after stimulation with thrombin, whereas only phosphatidylinositol was hydrolyzed with production of thromboxane B2 after stimulation with collagen. At high Ca2+ concentrations, phosphatidylcholine and diacylphosphatidylethanolamine were hydrolyzed after stimulation with collagen, and phosphatidylserine and alkenylacylphosphatidylethanolamine were degraded after stimulation with both thrombin and collagen. [1-14C]Arachidonic acid was heterogeneously incorporated into the individual molecular species of the various phospholipid classes, indicating that the determination of mass is essential for an accurate picture of phospholipid hydrolysis. The data reported here indicate that the Ca2+ concentration affects the differential degradation of phospholipid molecular species in activated human platelets.
mesh_terms: D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D002855:Chromatography, Thin Layer; D003094:Collagen / Q000494:pharmacology*; D006801:Humans; D006868:Hydrolysis; D010713:Phosphatidylcholines / Q000378:metabolism; D010716:Phosphatidylinositols / Q000378:metabolism; D010718:Phosphatidylserines / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism*; D010974:Platelet Aggregation / Q000187:drug effects*; D013917:Thrombin / Q000494:pharmacology*; D010738:Type C Phospholipases / Q000378:metabolism
chemical_list: D001095:Arachidonic Acids; D010713:Phosphatidylcholines; D010716:Phosphatidylinositols; D010718:Phosphatidylserines; D010743:Phospholipids; D016718:Arachidonic Acid; D003094:Collagen; D010738:Type C Phospholipases; D013917:Thrombin; D002118:Calcium