The effects of cytokines and adherent cells on the interleukin 4-mediated induction of Ia antigens on resting B cells.
In this report we have extended our previous studies on interleukin 4 (IL-4) [previously termed B-cell stimulatory factor-1 (BSF-1)]. Our results demonstrate that 8 hr of exposure to IL-4 is sufficient to induce maximal expression of Ia antigens. This increase in expression of Ia antigens on resting B cells is due to the direct action of IL-4 on the B cells since adding or removing adherent cells or utilizing low density cultures of B cells at 50-100/culture had no effect on the IL-4-mediated increase in Ia. Monoclonal anti-IL-4 antibody completely abrogated the Ia-inducing activity of IL-4. A variety of other purified lymphokines including interleukin 2 (IL-2), interleukin 1 (IL-1), and a source of either B-cell differentiation factor for IgM (BCDF mu), or B-cell growth factor II (BCGF II), did not alter the expression of Ia antigens on resting B cells. However, interferon-gamma can partially inhibit the IL-4-mediated induction of Ia.
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D001688:Biological Products / Q000502:physiology*; D016207:Cytokines; D004305:Dose-Response Relationship, Drug; D005260:Female; D006131:Growth Inhibitors; D006133:Growth Substances / Q000502:physiology*; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D007371:Interferon-gamma / Q000494:pharmacology; D015847:Interleukin-4; D008222:Lymphokines / Q000037:antagonists & inhibitors / Q000502:physiology*; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D013601:T-Lymphocytes / Q000276:immunology
D001688:Biological Products; D016207:Cytokines; D006131:Growth Inhibitors; D006133:Growth Substances; D000949:Histocompatibility Antigens Class II; D008222:Lymphokines; D015847:Interleukin-4; D007371:Interferon-gamma