Studies on internalization and intracellular localization of membrane DNA in mouse peritoneal macrophages, by means of lactoferrin-gold complex.
It is an experimental fact that lactoferrin binds to DNA. In the present study, a lactoferrin-gold complex was first bound by the cell surface, then became internalized (inside coated or smooth vesicles). Intracellularly the complex appeared in tube-like or vesicle-like structures, which approximated the nucleus, and some vesicles even showed indications of association with the external nuclear membrane. Neither membrane binding, nor internization of the lactoferrin-gold complex had taken place in cells pretreated with DNase.
D000818:Animals; D002462:Cell Membrane / Q000648:ultrastructure; D002478:Cells, Cultured; D004247:DNA / Q000032:analysis*; D006046:Gold / Q000378:metabolism / Q000494:pharmacology; D007425:Intracellular Membranes / Q000648:ultrastructure; D007781:Lactoferrin / Q000378:metabolism / Q000494:pharmacology; D007782:Lactoglobulins; D008264:Macrophages / Q000648:ultrastructure*; D051379:Mice; D008815:Mice, Inbred Strains; D008854:Microscopy, Electron; D010529:Peritoneal Cavity / Q000166:cytology*
D007782:Lactoglobulins; D006046:Gold; D004247:DNA; D007781:Lactoferrin