malM, a new gene of the maltose regulon in Escherichia coli K12. II. Mutations affecting the signal peptide of the MalM protein.
malM is the last gene of the malK-lamB-malM operon of Escherichia coli K12. It encodes a periplasmic protein. Mutations affecting the hydrophobic core of the N-terminal extension of the MalM protein have been isolated. They result in an increase in amount and specific activity of a MalM-LacZ hybrid protein. This result confirms that the signal peptide of the MalM protein is functional.
D001483:Base Sequence; D004269:DNA, Bacterial; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005798:Genes, Bacterial; D008320:Maltose / Q000235:genetics*; D009154:Mutation; D010641:Phenotype; D014176:Protein Biosynthesis; D021382:Protein Sorting Signals / Q000235:genetics*; D013728:Terminator Regions, Genetic; D001616:beta-Galactosidase / Q000378:metabolism
D004269:DNA, Bacterial; D021382:Protein Sorting Signals; D008320:Maltose; D001616:beta-Galactosidase