Tyrosination-detyrosination of the COOH-terminus of alpha-tubulin in oocytes and embryos of Bufo arenarum.
Soluble tubulin from Bufo arenarum oocytes and early embryos was shown to be composed mainly of the non-tyrosinable species. The low proportion of tyrosinable tubulin was almost exclusively constituted by the tyrosinated form. Compared with oocytes and embryos, toad brain contained a higher proportion of tyrosinable tubulin constituted mainly by the non-tyrosinated form. Tubulin carboxypeptidase was detected in toad brain but not in oocytes and embryos.
D000818:Animals; D001921:Brain / Q000201:enzymology; D002022:Bufo arenarum / Q000378:metabolism*; D002250:Carbon Radioisotopes; D002268:Carboxypeptidases / Q000378:metabolism; D004625:Embryo, Nonmammalian / Q000201:enzymology*; D009865:Oocytes / Q000201:enzymology*; D014404:Tubulin / Q000378:metabolism*; D014443:Tyrosine / Q000378:metabolism*
D002250:Carbon Radioisotopes; D014404:Tubulin; D014443:Tyrosine; D002268:Carboxypeptidases; C023212:tyrosyltubulin carboxypeptidase