Enkephalin convertase in the rat spinal cord.
3H-Guanidinoethylmercaptosuccinic acid (3H-GEMSA) a very selective inhibitor of enkephalin convertase, binds to the crude rat spinal cord homogenates saturably, reversibly and with high affinity. Scatchard analysis revealed two classes of binding sites with KD: 4.5 nM and 215 nM. A plot of dissociation experiment was nonlinear with the T1/2: 2 min and 6 min, respectively. 3H-GEMSA binding sites are evenly distributed throughout the rat spinal cord and their high density might suggest a physiological significance of enkephalin convertase in that tissue.
D000818:Animals; D043423:Carboxypeptidase H; D002268:Carboxypeptidases / Q000378:metabolism*; D007700:Kinetics; D011485:Protein Binding; D051381:Rats; D011919:Rats, Inbred Strains; D013116:Spinal Cord / Q000201:enzymology*; D013386:Succinates / Q000378:metabolism
D013386:Succinates; C022014:guanidinoethylmercaptosuccinic acid; D002268:Carboxypeptidases; D043423:Carboxypeptidase H