An active-site titrant for human tissue-type plasminogen activator.
The reaction of recombinant tissue-type plasminogen activator with the inverse substrate 4-amidino-2-nitrophenyl 4'-anisate results in the rapid release of the chromogen 4-amidino-2-nitrophenol and the accumulation of the relatively stable 4-anisoyl-enzyme. Spectrophotometric monitoring of the reaction enables the operational molarity of the enzyme to be determined.
D001565:Benzoates / Q000378:metabolism*; D001665:Binding Sites; D007700:Kinetics; D011994:Recombinant Proteins / Q000378:metabolism; D013053:Spectrophotometry; D010959:Tissue Plasminogen Activator / Q000378:metabolism*
D001565:Benzoates; D011994:Recombinant Proteins; C051327:4-amidino-2-nitrophenyl 4'-anisate; D010959:Tissue Plasminogen Activator