title: Changes of tissue blood flow in mice loaded with SART (repeated cold) stress or restraint and water immersion stress and the effect of administered neurotropin.
abstract: In order to explore the peripheral microcirculation and to obtain an outline of autonomic innervation in SART (specific alternation of rhythm in temperature)-stressed (repeated cold-stressed) animals, which are regarded as model animals for clinical vagotonic-type dysautonomia, peripheral tissue blood flow was determined in mice, using the hydrogen clearance method. SART-stressed mice showed a decrease in gastric blood flow, no change in hepatic blood flow and an increase in dermal blood flow. In the mice exposed to the restraint and water immersion stress (RWIS), a type of acute stress, in contrast with SART stress which is a subacute type, remarkable decreases were observed in gastric, hepatic and dermal blood flows. Changes of both gastric and dermal blood flow in SART-stressed mice were dose-dependently prevented and maintained within normal limits by the treatment with Neurotropin, a sedative analgesic which is an extract isolated from vaccinia virus-inoculated and inflamed skin of rabbits. In RWIS-loaded mice, Neurotropin exhibited a great preventive effect on changes of blood flow in the stomach, a slight effect in the liver, and no effect in the cutis. When mice were loaded with SART stress after left-cervical vagotomy, SART stress failed to elicit any decrease in gastric blood flow. In SART-stressed mice treated with 6-hydroxydopamine, gastric and dermal blood flows tended to show a further decrease and increase, respectively, over and above the changes caused by SART stress. From these results, it is suggested that SART-stressed mice may have decreased gastric parasympathetic tone, a decrease in sympathetic tone and also other anomalies such as increased tension of the sympathetic cholinergic vasodilator nerves in the cutis.
mesh_terms: D000818:Animals; D003080:Cold Temperature; D006892:Hydroxydopamines; D008102:Liver Circulation / Q000187:drug effects; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D016627:Oxidopamine; D011134:Polysaccharides / Q000494:pharmacology*; D012039:Regional Blood Flow / Q000187:drug effects; D012149:Restraint, Physical; D012867:Skin / Q000098:blood supply; D013270:Stomach / Q000098:blood supply; D013312:Stress, Physiological / Q000503:physiopathology*; D013315:Stress, Psychological / Q000503:physiopathology*; D013563:Sympathectomy, Chemical; D014628:Vagotomy; D014867:Water
chemical_list: D006892:Hydroxydopamines; D011134:Polysaccharides; D014867:Water; C010491:neurotropin; D016627:Oxidopamine