Detection of inhibitory inactive fraction of alpha-1-antitrypsin in human serum.
Pseudomonas aeruginosa elastase is not inhibited by human serum alpha-1-antitrypsin, it damages inhibitory activity of alpha-1-antitrypsin activity instead. A simple method, based on electrophoresis in urea containing polyacrylamide gel, is described for the separation of active part of the inhibitor from that inactivated by the bacterial enzyme.
D001665:Binding Sites; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007123:Immunoelectrophoresis, Two-Dimensional; D010196:Pancreatic Elastase / Q000494:pharmacology*; D011550:Pseudomonas aeruginosa / Q000201:enzymology; D000515:alpha 1-Antitrypsin / Q000037:antagonists & inhibitors* / Q000302:isolation & purification
D000515:alpha 1-Antitrypsin; D010196:Pancreatic Elastase