MHC antigen expression on bulk isolated macrophage-microglia from newborn mouse brain: induction of Ia antigen expression by gamma-interferon.
Macrophage-microglia were isolated from primary mixed brain cell cultures of normal newborn mice. They were successfully maintained in vitro for at least 8 weeks. Purity of the cultures was 97-100%, as determined by endocytosis of latex beads, non-specific staining through Fc receptors, EA and EAC rosette formation. These cells were non-specific esterase-positive, but peroxidase-negative. Electron-microscope observations revealed morphological similarities to mature macrophages. Isolated macrophage-microglia seldom incorporated [3H]thymidine in vitro. By means of 51Cr release assay, using monoclonal antibodies against mouse major histocompatibility complex (MHC) antigens and complement, we detected class I MHC (H-2) antigen on unstimulated macrophage-microglia, and both class I and class II (Ia) antigens on gamma-interferon-treated cells. These observations suggest possible immunoregulatory functions of macrophage-microglia in the central nervous system, as is characteristic of other cells of monocyte lineage.
D000818:Animals; D000831:Animals, Newborn; D001345:Autoradiography; D001921:Brain / Q000276:immunology; D002455:Cell Division; D002478:Cells, Cultured; D006183:H-2 Antigens / Q000032:analysis*; D000949:Histocompatibility Antigens Class II / Q000032:analysis*; D007371:Interferon-gamma / Q000494:pharmacology*; D008264:Macrophages / Q000276:immunology* / Q000648:ultrastructure; D051379:Mice; D008806:Mice, Inbred AKR; D008807:Mice, Inbred BALB C; D008854:Microscopy, Electron; D009457:Neuroglia / Q000032:analysis* / Q000648:ultrastructure
D006183:H-2 Antigens; D000949:Histocompatibility Antigens Class II; D007371:Interferon-gamma