Gadolinium and didymium (praseodymium/neodymium) cations as capture agents in lightmicroscopical histochemistry of acid and alkaline phosphatase.
In previous papers, cerium and lanthanum based methods for light-microscopical detection of acid and alkaline phosphatase activity were proposed. In this paper, the usefulness of other lanthanide cations such as gadolinium and praseodymium/neodymium cations as capture agents in phosphatase histochemistry is tested. It is evident that phosphate ions were sufficiently trapped by these cations. According to the lead and silver multistep procedures earlier described it is possible to visualize alkaline phosphatase activity in the brush borders of the intestine or kidney as well as acid phosphatase activity in the lysosomes. These methods can be recommended.
D000135:Acid Phosphatase / Q000378:metabolism*; D000469:Alkaline Phosphatase / Q000378:metabolism*; D000818:Animals; D002412:Cations; D005260:Female; D006651:Histocytochemistry; D007202:Indicators and Reagents; D007422:Intestines / Q000166:cytology / Q000201:enzymology*; D007668:Kidney / Q000166:cytology / Q000201:enzymology*; D008297:Male; D009354:Neodymium; D011221:Praseodymium; D051381:Rats; D011919:Rats, Inbred Strains
D002412:Cations; D007202:Indicators and Reagents; D009354:Neodymium; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D011221:Praseodymium