Biophysical characterization of osteoporotic bone.
A number of biophysical and biochemical parameters have been measured in normal and osteoporotic animals and their bones. Rats have been fed normal and calcium- and phosphorus-deficient diets (through mineral content) from the weaning period to maturity (greater than 125 days). The experiments were performed after attaining this age. Biochemical parameters considered for comparison included: red blood cells (RBC), white blood cells (WBC), Hb, serum calcium and phosphorus, blood pH, PCO2, and PO2. Bones obtained after sacrificing mature animals were used for obtaining data regarding infrared, fluorescence, and X-ray spectra, and transmission electron micrographs, and were also subjected to spectrochemical analysis. It was found that the two sets of results show differences in most of the above-mentioned parameters, which correlates well with the loss of calcium and phosphorus metabolism and the corresponding increase in the amorphous-to-crystalline ratio. It is hoped that these data will be useful in characterizing the osteoporotic bone and will suggest the mechanism for reversal by external stimulations.
D000818:Animals; D001842:Bone and Bones / Q000032:analysis / Q000378:metabolism*; D002118:Calcium / Q000032:analysis / Q000097:blood / Q000378:metabolism*; D002245:Carbon Dioxide / Q000097:blood; D004032:Diet; D006863:Hydrogen-Ion Concentration; D008855:Microscopy, Electron, Scanning; D010024:Osteoporosis / Q000378:metabolism*; D010100:Oxygen / Q000097:blood; D010758:Phosphorus / Q000032:analysis / Q000097:blood / Q000378:metabolism*; D051381:Rats; D013055:Spectrophotometry, Infrared; D014131:Trace Elements / Q000032:analysis
D014131:Trace Elements; D002245:Carbon Dioxide; D010758:Phosphorus; D010100:Oxygen; D002118:Calcium