title: Ca2+-dependent translocation of hexose carrier in mouse fibroblast Swiss 3T3 cells.
abstract: Ca2+-induced translocation of hexose carriers from microsomal membrane to plasma membrane was demonstrated in saponin-permeabilized Swiss 3T3 cells by a specific D-glucose-inhibitable cytochalasin B-binding assay. The number of hexose carriers in the plasma membrane and the hexose transport activity in intact cells were also compared. The incubation of permeabilized cells with 10 microM Ca2+ at 37 degrees C rapidly increased the number of D-glucose-inhibitable cytochalasin B-binding sites in the plasma membrane from 13 to 40 pmol/mg protein and concomitantly decreased that in the microsomal membrane from 66 to 36 pmol/mg protein, each with a half-time of approx. 2 min. Furthermore, when Ca2+-stimulated cells were exposed to 50 microM EGTA, the effect of Ca2+ on the translocation of D-glucose-inhibitable cytochalasin B-binding sites was reversed with a half-time of approx. 5 min. The concentration of Ca2+ required for the half-maximal effect was approx 500 nM. The magnitude of the stimulatory effect of D-glucose-inhibitable cytochalasin B-binding sites in the plasma membrane closely correlated with the magnitude of stimulatory action of Ca2+ on 3-O-methylglucose transport in the intact cells. These results suggest that Ca2+ regulates the activity of hexose transport across the plasma membrane through a rapid and reversible translocation of hexose carrier between microsomal and plasma membranes of mouse fibroblast Swiss 3T3 cells.
mesh_terms: D019325:3-O-Methylglucose; D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D002147:Calmodulin / Q000037:antagonists & inhibitors; D002460:Cell Line; D002463:Cell Membrane Permeability / Q000187:drug effects; D003571:Cytochalasin B; D004136:Dinitrobenzenes / Q000494:pharmacology; D007295:Inositol Phosphates / Q000494:pharmacology; D008757:Methylglucosides / Q000378:metabolism; D051379:Mice; D009004:Monosaccharide Transport Proteins / Q000378:metabolism*; D012503:Saponins / Q000494:pharmacology
chemical_list: D002147:Calmodulin; D004136:Dinitrobenzenes; D007295:Inositol Phosphates; D008757:Methylglucosides; D009004:Monosaccharide Transport Proteins; D012503:Saponins; D019325:3-O-Methylglucose; D000001:Calcimycin; D003571:Cytochalasin B; D002118:Calcium