Characterization of human IMR-90 fibroblasts as a model system for the study of chemical carcinogenesis in vitro.
The effects of chemical carcinogens on various aspects of cellular metabolism were measured in human IMR-90 fibroblasts. These studies were undertaken to assess the ability of IMR-90 cells to be used for the study of chemical carcinogenesis in vitro. It was found that chemical carcinogens inhibited DNA synthesis in synchronous cultures of IMR-90 fibroblasts; the effective concentrations of carcinogens showing effects in this system were lower than for other published in vitro cell assay systems; compounds that require metabolic activation to a carcinogenic form demonstrated the ability to inhibit DNA synthesis in IMR-90 fibroblasts; a fluorometric determination of aryl hydrocarbon hydroxylase (AHH) activity in IMR-90 cells revealed a low level of inducible AHH; IMR-90 cells can metabolize 7,12-dimethylbenzanthracene (DMBA) to compounds that are identified as metabolites of DMBA in vivo.
D015127:9,10-Dimethyl-1,2-benzanthracene / Q000494:pharmacology; D001189:Aryl Hydrocarbon Hydroxylases / Q000378:metabolism; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D004261:DNA Replication / Q000187:drug effects; D005347:Fibroblasts / Q000187:drug effects*; D006801:Humans; D009152:Mutagenicity Tests / Q000379:methods
D015127:9,10-Dimethyl-1,2-benzanthracene; D001189:Aryl Hydrocarbon Hydroxylases