Solubilization of protein BM-40 from a basement membrane tumor with chelating agents and evidence for its identity with osteonectin and SPARC.
Up to 80% of the calcium-binding protein BM-40 could be extracted from a tumor basement membrane with a physiological buffer containing 10 mM EDTA. About half of its amino acid sequence was determined by Edman degradation demonstrating identity with cDNA deduced sequences of bone osteonectin and SPARC.
D000595:Amino Acid Sequence; D000818:Animals; D001485:Basement Membrane / Q000032:analysis* / Q000187:drug effects; D002135:Calcium-Binding Proteins / Q000302:isolation & purification*; D002352:Carrier Proteins / Q000235:genetics / Q000302:isolation & purification*; D004247:DNA / Q000235:genetics; D004492:Edetic Acid / Q000494:pharmacology; D051379:Mice; D009363:Neoplasm Proteins / Q000302:isolation & purification; D009374:Neoplasms, Experimental / Q000032:analysis; D015676:Osteonectin
D002135:Calcium-Binding Proteins; D002352:Carrier Proteins; D009363:Neoplasm Proteins; D015676:Osteonectin; D004247:DNA; D004492:Edetic Acid