Efficient radiolabeling of mammalian cells using 111In-tagged liposomes.
When indium-111 oxine labeled neutral liposomes were incubated with Chinese hamster V79 cells in the presence of 100 mM calcium, the cell-associated radioactivity increased approximately 75-fold over that observed in the absence of calcium. This is considerably higher (approximately 20 times) than the cellular uptake obtained when these cells are incubated in the presence of 111In-oxine alone. The highest uptake of radioactivity occurred when no bovine serum albumin was present in the medium, while as little as 0.001% of the protein greatly reduced the cell-liposome association. These efficient cell labeling conditions were not found to affect the survival of the cells.
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002460:Cell Line; D006912:Hydroxyquinolines / Q000378:metabolism*; D007205:Indium Radioisotopes; D007553:Isotope Labeling; D008081:Liposomes; D009942:Organometallic Compounds / Q000378:metabolism*; D015125:Oxyquinoline / Q000031:analogs & derivatives / Q000378:metabolism*; D012710:Serum Albumin, Bovine / Q000494:pharmacology
D006912:Hydroxyquinolines; D007205:Indium Radioisotopes; D008081:Liposomes; D009942:Organometallic Compounds; C032950:indium oxine; D012710:Serum Albumin, Bovine; D015125:Oxyquinoline; D002118:Calcium