beta-Glucosidase inhibition in murine peritoneal macrophages by conduritol-B-epoxide: an in vitro model of the Gaucher cell.
Murine peritoneal macrophages were cultured in the presence of conduritol-B-epoxide, a specific covalent inhibitor of beta-glucosidase. The inhibition was found to be dose and time dependent. Upon removal of the inhibitor from the culture medium, beta-glucosidase activity recovered to half maximum by 2.2 days. Treatment of macrophages with this inhibitor for 15 days did not affect cell viability, lysosomal enzyme release to the medium, or levels of intracellular lysosomal enzymes, other than beta-glucosidase activity. This inhibition results in the accumulation of glucocerebroside. In vitro studies on the pathobiology of such macrophages whose beta-glucosidase activity has been reduced may be useful toward understanding the pathogenesis of Gaucher disease.
D000818:Animals; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005776:Gaucher Disease / Q000201:enzymology; D005959:Glucosidases / Q000037:antagonists & inhibitors*; D005963:Glucosylceramides / Q000378:metabolism; D007294:Inositol / Q000031:analogs & derivatives* / Q000494:pharmacology; D008264:Macrophages / Q000201:enzymology*; D051379:Mice; D008954:Models, Biological; D010537:Peritoneum; D013997:Time Factors; D001617:beta-Glucosidase / Q000037:antagonists & inhibitors*
D005963:Glucosylceramides; D007294:Inositol; D005959:Glucosidases; D001617:beta-Glucosidase; C011423:conduritol epoxide