Effects of retinoic acid treatment on release of arachidonic acid by chondrogenic cells in response to ionophore A23187.
Chondrogenic differentiation in mouse limb bud mesenchymal cells cultured at high density was suppressed by supplementation of the medium with retinoic acid in a dose-dependent fashion. Cells prelabeled with (3H) arachidonic acid were treated with 0.3 microgram/ml retinoic acid. Treatment with retinoic acid increased the (3H) fatty acid in the triglyceride fraction. Furthermore, treatment with retinoic acid enhanced the release of (3H) fatty acid upon stimulation of these cells with the divalent ionophore A23187. These data permit the suggestion that there may be a correlation between altered lipid metabolism and retinoic acid's ability to disrupt chondrogenic differentiation.
D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism*; D000001:Calcimycin / Q000494:pharmacology*; D002118:Calcium / Q000494:pharmacology; D002356:Cartilage / Q000187:drug effects / Q000378:metabolism*; D002478:Cells, Cultured; D004622:Embryo, Mammalian; D005121:Extremities; D007700:Kinetics; D051379:Mice; D014212:Tretinoin / Q000494:pharmacology*; D014316:Tritium
D001095:Arachidonic Acids; D014316:Tritium; D016718:Arachidonic Acid; D000001:Calcimycin; D014212:Tretinoin; D002118:Calcium