In vitro aldose reductase inhibitory activity of substituted N-benzenesulfonylglycine derivatives.
A number of N-benzenesulfonylglycines, alanines, sarcosine, and prolines, which contain the minimum pharmacophore moieties necessary for aldose reductase inhibitory activity, were prepared and tested in the rat lens assay. In this assay, the benzenesulfonylglycines are considerably more potent than the corresponding alanine and sarcosine derivatives which, in turn, are more active than the proline analogues. Of the monosubstituted benzenesulfonylglycines, the 2-nitro and 4-amino derivatives were most active with 50% inhibitory concentration (IC50) values of 13 and 16 microM, respectively. The most potent derivatives evaluated were the beta- and alpha-naphthylenesulfonylglycines with IC50 values of 0.4 and 1.3 microM, respectively. The structure-activity data obtained from evaluation of the benzenesulfonylamino acids suggests that the aromatic ring and ring substituents, as well as the sulfonamide group and carboxylate moiety, all contribute to the inhibitory potency through direct interaction with complimentary binding sites present on aldose reductase.
D000449:Aldehyde Reductase / Q000037:antagonists & inhibitors*; D000596:Amino Acids; D000818:Animals; D001557:Benzenesulfonates / Q000138:chemical synthesis / Q000494:pharmacology*; D066298:In Vitro Techniques; D007908:Lens, Crystalline / Q000201:enzymology; D051381:Rats; D013329:Structure-Activity Relationship; D013401:Sugar Alcohol Dehydrogenases / Q000037:antagonists & inhibitors*
D000596:Amino Acids; D001557:Benzenesulfonates; D013401:Sugar Alcohol Dehydrogenases; D000449:Aldehyde Reductase