Progesterone secretion by primary cultures of rat luteal cells.
Primary cultures were prepared from ovaries of immature rats that had been superovulated. The dispersed luteal cells attached to growth surfaces, formed monolayers and secreted progesterone. Progesterone accumulation in the medium was most pronounced in the first week of culturing. Removal of serum from the medium resulted in a progressive decline in progesterone concentration in the culture medium which reached basal levels by 5 hours. In medium without serum, the addition of hCG, FSH or prolactin stimulated an increase in progesterone secretion within 1 hour. Also cholera toxin stimulated a significant increase in progesterone levels in the medium. Prior exposure of cultures to estradiol for 3 days did not augment the response to hCG and inhibited the ability of cholera toxin to stimulate progesterone secretion. These results indicate that the steroidogenic function of rat luteal cells can be studied in culture and that a number of hormones rapidly stimulate the secretion of progesterone from these cells.
D000818:Animals; D002470:Cell Survival; D002478:Cells, Cultured; D002772:Cholera Toxin / Q000494:pharmacology; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D003338:Corpus Luteum / Q000378:metabolism*; D003470:Culture Media; D004347:Drug Interactions; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D008184:Luteal Cells / Q000378:metabolism*; D011374:Progesterone / Q000378:metabolism*; D011388:Prolactin / Q000494:pharmacology; D051381:Rats; D012636:Secretory Rate / Q000187:drug effects
D006063:Chorionic Gonadotropin; D003470:Culture Media; D011374:Progesterone; D004958:Estradiol; D011388:Prolactin; D005640:Follicle Stimulating Hormone; D002772:Cholera Toxin