B lymphocyte-specific protein binding near an immunoglobulin kappa-chain gene J segment.
Nuclear extracts from pre-B and B cell lines contain a nuclear DNA binding protein (kappa locus protein, KLP) that specifically recognizes a DNA sequence in the immunoglobulin kappa light chain joining (J) segment gene region. KLP is not observed in mature B cells, T cells, or nonlymphoid cell types. Two tandem binding sites for KLP designated KI and KII have been identified by methylation interference analysis to be immediately proximal to the J kappa 1 nonamer-heptamer recognition sequences and separated by 38 base pairs from each other. Fragments of DNA containing KI and KII sites compete for binding to KLP, and both protein-DNA complexes have the same electrophoretic mobility. Other flanking sequences of immunoglobulin gene fragments do not bind to KLP. The position of KLP-DNA binding and its tissue-specific expression suggest that it may be involved in the regulation of lymphoid gene DNA rearrangements by targeting recombinase to the kappa-chain gene region.
D000818:Animals; D001402:B-Lymphocytes / Q000032:analysis*; D001483:Base Sequence; D001665:Binding Sites; D002460:Cell Line; D004247:DNA / Q000032:analysis; D004268:DNA-Binding Proteins / Q000032:analysis*; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D007145:Immunoglobulin kappa-Chains / Q000235:genetics*; D008745:Methylation; D051379:Mice; D011485:Protein Binding; D011995:Recombination, Genetic
D004268:DNA-Binding Proteins; D007135:Immunoglobulin Variable Region; D007145:Immunoglobulin kappa-Chains; D004247:DNA