Murine monoclonal antibody suitable for use as an Rh reagent, anti-e.
The production by the hybridoma technique of a monoclonal antibody which behaves as an anti-e agglutinin is described. A reagent was made from ascitic fluid diluted in RPMI 1640 culture medium and bovine serum albumin. All red blood cells bearing the e antigen were agglutinated; no reactivity was recorded with untreated EE red cells suspensions. However, cross-reactivity was observed with enzyme-pretreated EE cells. The characteristics of this monoclonal antibody are described and are in accordance with the standard references.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001788:Blood Grouping and Crossmatching; D003429:Cross Reactions; D006801:Humans; D007202:Indicators and Reagents; D051379:Mice; D012204:Rh-Hr Blood-Group System / Q000276:immunology*
D000911:Antibodies, Monoclonal; D007202:Indicators and Reagents; D012204:Rh-Hr Blood-Group System