Should platelets be radiolabelled in plasma medium?
Because of the low labelling efficiency of platelets with In-111 oxine in plasma containing media, most investigators have labelled platelets in plasma-free media. However, labelling of platelets with In-111 oxine in plasma-free media may result in either: irreversible damage leading to rapid clearance from the circulation and permanent hepatic sequestration; or reversible damage with transient hepatic sequestration without affecting the in vivo survival. Since human platelets can be labelled with In-111 oxine in plasma in sufficient efficiency for routine clinical use, they should be labelled in plasma whenever possible. The use of newer In-111 chelates, tropolone and mercaptopyridine-N-oxide, which label platelets with high efficiency in plasma as well as in plasma-free media, may offer another alternative.
D000818:Animals; D001792:Blood Platelets / Q000187:drug effects*; D002470:Cell Survival; D006801:Humans; D007205:Indium Radioisotopes / Q000494:pharmacology; D007553:Isotope Labeling / Q000379:methods*; D009942:Organometallic Compounds / Q000494:pharmacology; D015125:Oxyquinoline / Q000031:analogs & derivatives / Q000494:pharmacology; D010949:Plasma / Q000502:physiology; D011725:Pyridines / Q000494:pharmacology; D011817:Rabbits; D013871:Thiones; D014334:Tropolone / Q000494:pharmacology
D007205:Indium Radioisotopes; D009942:Organometallic Compounds; D011725:Pyridines; D013871:Thiones; C032950:indium oxine; D015125:Oxyquinoline; C008704:pyrithione; D014334:Tropolone