Single-stranded plasmid DNA in Bacillus subtilis and Staphylococcus aureus.
Plasmid pC194 was found to exist in a double-stranded and a single-stranded DNA form in Bacillus subtilis and Staphylococcus aureus. This single-stranded DNA was found as a circular molecule of the same size as the parental monomer and corresponded to only one of the two DNA strands. It represented one-third of plasmid copies. Single- and double-stranded DNA copies in similar proportions to the above were detected for five other S. aureus plasmids (pC221, pC223, pE194, pT127, and pT181) and one B. subtilis plasmid (pHV416). S. aureus plasmid pUB110 and Bacillus cereus plasmid pBC16 were, in contrast, predominantly double-stranded.
D001412:Bacillus subtilis / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D004270:DNA, Circular / Q000235:genetics; D004277:DNA, Single-Stranded / Q000235:genetics*; D008854:Microscopy, Electron; D010957:Plasmids; D013211:Staphylococcus aureus / Q000235:genetics*
D004269:DNA, Bacterial; D004270:DNA, Circular; D004277:DNA, Single-Stranded