[Selective interaction of immunoglobulin polypeptide chains. Quantitative evaluation of the idiotypic and antigen binding activity of reassociated immunoglobulin molecules].
We have analysed reassociated Ig molecules, containing heavy (H) or light (L) chains of Ig-B2 monoclonal antibody with human fibronectin binding activity and L of H chains of normal mouse serum immunoglobulin (Ig-NM). Examination of Ig-B2 idiotype expression in reassociated Ig indicated that 0.4% L-NM and 0.8% H-NM were able to restore Ig-B2 idiotype. The analysis of antigen binding capacity of reassociated Ig demonstrated, that only 4% H-NM created antigen binding site in complex with L-B2. We have determined the leading role of L-chain in creation of idiotype and binding site of Ig-B2. Selectivity of interaction between H and L chains is discussed. The results indicate, that not more than 4-6% of random H--L combinations produce functional Ig.
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001666:Binding Sites, Antibody; D005353:Fibronectins / Q000276:immunology; D006801:Humans; D006825:Hybridomas; D007074:Immunoglobulin G / Q000276:immunology*; D007143:Immunoglobulin Heavy Chains / Q000276:immunology / Q000302:isolation & purification; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D007147:Immunoglobulin Light Chains / Q000276:immunology / Q000302:isolation & purification; D051379:Mice; D010455:Peptides / Q000276:immunology*
D000911:Antibodies, Monoclonal; D005353:Fibronectins; D007074:Immunoglobulin G; D007143:Immunoglobulin Heavy Chains; D007130:Immunoglobulin Idiotypes; D007147:Immunoglobulin Light Chains; D010455:Peptides
Izbiratel'noe vzaimodeĭstvie polipeptidnykh tsepeĭ immunoglobilinov.Kolichestvennaia otsenka idiotipicheskoĭ i antigensviazyvaiushcheĭ aktivnosti reassotsiirovannykh molekul immunoglobulinov.