[Simultaneous determination of specific antigen, antibody and immune complexes in the serum of patients with chronic schistosomiasis].
The main steps of a sensitive and quantitative technique for the simultaneous determination, in the same microplate, of antigen, antibody and specific immune complexes has been described. The assay involved principles of the double sandwich technique used in the "Enzyme-Linked Immunosorbent Assay" (ELISA). The technique proved to be useful for the estimation of antigen, antibody and specific immune complexes in sera of patients with human chronic hepato-intestinal schistosomiasis.
D000293:Adolescent; D000328:Adult; D000906:Antibodies / Q000032:analysis*; D000936:Antigen-Antibody Complex / Q000032:analysis*; D000947:Antigens, Helminth / Q000032:analysis*; D002648:Child; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D012550:Schistosoma mansoni / Q000276:immunology*; D012555:Schistosomiasis mansoni / Q000276:immunology*
D000906:Antibodies; D000936:Antigen-Antibody Complex; D000947:Antigens, Helminth
Pesquisa simultãnea de antígeno, anticorpo e imunocomplexos específicos no soro de pacientes com esquistossomose crõnica.