Adenine nucleotide compartmentation in foetal rat hepatocytes. Effects of atractyloside, oligomycin, calcium ionophore and adrenergic agonists.
In the presence of lactate plus pyruvate, or glucose or alanine as substrates, ATP/ADP ratios in the cytosol were higher than in mitochondria in isolated rat foetal hepatocytes. The cytosolic ATP/ADP ratios were dependent on substrate (lactate + pyruvate greater than glucose greater than alanine). Oleate increased the cytosolic ATP/ADP ratios in the presence of the other substrates studied. Atractyloside decreased the cytosolic ATP/ADP ratios, oligomycin decreasing these values in both compartments. Isoproterenol, phenylephrine and Ca2+ ionophore decreased the cytosolic ATP/ADP ratio, without altering this value in mitochondria.
D000227:Adenine Nucleotides / Q000378:metabolism*; D000818:Animals; D001278:Atractyloside / Q000494:pharmacology; D000001:Calcimycin / Q000494:pharmacology; D002451:Cell Compartmentation / Q000187:drug effects*; D003600:Cytosol / Q000378:metabolism; D005333:Fetus / Q000378:metabolism; D008099:Liver / Q000196:embryology / Q000378:metabolism*; D008930:Mitochondria, Liver / Q000378:metabolism; D009840:Oligomycins / Q000494:pharmacology; D010085:Oxidative Phosphorylation / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains; D013566:Sympathomimetics / Q000494:pharmacology
D000227:Adenine Nucleotides; D009840:Oligomycins; D013566:Sympathomimetics; D001278:Atractyloside; D000001:Calcimycin