[Effects of DTT and thioredoxins on rat argininosuccinate synthetase activity in vitro].
Argininosuccinate synthetase (ASS, EC 6.3.4.5), the third enzyme of urea-cycle, was studied in desactivated extracts of rat liver. The enzyme is activated, in vitro, by Mg2+ ions (5 mM) and dithiothreitol (DTT: 10 mM). After reduction by DTT, thioredoxins isolated from rat liver were able to activate ASS by 370%.
D000818:Animals; D001124:Argininosuccinate Synthase / Q000378:metabolism*; D001426:Bacterial Proteins / Q000494:pharmacology*; D004229:Dithiothreitol / Q000494:pharmacology*; D007700:Kinetics; D008025:Ligases / Q000378:metabolism*; D008099:Liver / Q000201:enzymology*; D008274:Magnesium / Q000494:pharmacology; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013879:Thioredoxins / Q000494:pharmacology*
D001426:Bacterial Proteins; D013879:Thioredoxins; D008025:Ligases; D001124:Argininosuccinate Synthase; D008274:Magnesium; D004229:Dithiothreitol
Effets du DTT et des thiorédoxines sur l'activité de l'arginosuccinate synthétase chez le rat in vitro.