Specificity of a monoclonal antibody to Drosophila melanogaster neoplastic hematopoietic cell surfaces as detected by immunofluorescence.
We have generated, and characterized the immunological specificity of, a monoclonal antibody (MAb) termed 2B3G8 that recognizes cell-surface determinant(s) of D. melanogaster neoplastic hematopoietic tissue. This antibody was generated against antigens of a tumor line derived from neoplastic lymph glands, the hematopoietic organs, of a growth control mutant, Tumorous-lethal (Tum1). 2B3G8 does not recognize any of a panel of normal Drosophila cells or transformed cell lines from mouse or human sources as assayed by indirect immunofluorescence. Enzymatic digests did not remove this antigen from neoplastic blood cells or uncover the antigen in normal blood cells. Thus, we have generated a MAb that serves as a marker for the neoplastic hematopoietic condition in Drosophila which is a model for vertebrate neoplasia. This antibody is potentially useful for the study of oncodevelopmental antigens in developing hematopoietic systems and for detecting the onset of the neoplastic condition.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D004331:Drosophila melanogaster; D005455:Fluorescent Antibody Technique; D051379:Mice; D008807:Mice, Inbred BALB C; D008854:Microscopy, Electron; D014411:Neoplastic Stem Cells / Q000276:immunology*
D000911:Antibodies, Monoclonal