Photoreactivation of UV damage in cultured Drosophila cells.
Cell survival and photoreactivation of 254 nm ultraviolet (UV) light damage in a wild type Drosophila cell line was assayed by colony formation in liquid medium. Fo, Fq, and extrapolation number for the exponential portion of survival curves are 21 J/m2, 3.6 J/m2, and 1.5 for non-photoreactivated cells and 110 J/m2, 11.2 J/m2, and 1.3 for those exposed to photoreactivating light. Maximal photoreactivation occurs at the 100 J/m2 region of the curve. At 10 and 50% survival, 75-80% of the UV damage was photoreactivable.
D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000528:radiation effects; D004260:DNA Repair / Q000528:radiation effects*; D004331:Drosophila melanogaster / Q000166:cytology* / Q000235:genetics; D008027:Light; D014466:Ultraviolet Rays