Processing of Bacillus subtilis succinate dehydrogenase and cytochrome b-558 polypeptides. Lack of covalently bound flavin in the Bacillus enzyme expressed in Escherichia coli.
The DNA sequence of the Bacillus subtilis sdh operon coding for the two succinate dehydrogenase subunits and cytochrome b-558 (the membrane anchor protein) has recently been established. We have now determined the extent of N-terminal processing of each polypeptide by radiosequence analysis. At the same time, direct evidence for the correctness of the predicted reading frames has been obtained. The cytochrome showed a ragged N-terminus, with forms lacking one residue, and is inserted across the membrane without an N-terminal leader-peptide. Covalently bound flavin was not detectable in B. subtilis succinate dehydrogenase expressed in Escherichia coli despite normal N-terminal processing of the apoprotein. This provides an explanation to why the succinate dehydrogenase synthesized in E. coli is not functional and demonstrates that host-specific factors regulate the coenzyme attachment.
D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000201:enzymology* / Q000235:genetics; D001483:Base Sequence; D002462:Cell Membrane / Q000201:enzymology; D003573:Cytochrome b Group / Q000235:genetics / Q000378:metabolism*; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D005415:Flavins / Q000378:metabolism; D019255:NADPH Oxidases; D010446:Peptide Fragments; D011499:Protein Processing, Post-Translational; D013385:Succinate Dehydrogenase / Q000235:genetics / Q000378:metabolism*
D003573:Cytochrome b Group; D004269:DNA, Bacterial; D005415:Flavins; D010446:Peptide Fragments; C036888:cytochrome b558; D013385:Succinate Dehydrogenase; D019255:NADPH Oxidases