An evaluation of the polyacrylamide gel electrophoresis fractionation method for the production of Mycobacterium tuberculosis skin test preparations. I. Production, physiochemical characterization and serological analyses.
As a part of a cooperative inter-laboratory WHO supported project raw tuberculins were produced and purified protein derivative (PPD, 18.7 g protein) was prepared. Employing a multistage preparative polyacrylamide gel electrophoresis (PAGE) method the PPD was separated into four fractions corresponding to 15, 7, 4.75 and 3.5% gel concentrations. The PAGE procedure resulted in three lots of material--each representing 11 electrophoretic runs. Immunodiffusion analyses showed that the largest number of precipitinogens was found in the 15% fractions and that some precipitinogens cross-reacted with preparations of Mycobacterium bovis BCG, M. intracellulare, M. kansasii, M. smegmatis and M. vaccae.
D000907:Antibodies, Bacterial / Q000276:immunology; D000942:Antigens, Bacterial / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D005069:Evaluation Studies as Topic; D009169:Mycobacterium tuberculosis / Q000276:immunology; D014373:Tuberculin / Q000302:isolation & purification* / Q000592:standards; D014374:Tuberculin Test; D014944:World Health Organization
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D014373:Tuberculin