Presence of sucrase in the yolk sac of the chick.
The presence of sucrase in the yolk sac of the chick was studied biochemically and immunologically. The sucrase was partially purified from the yolk sac of hatched chicks and was compared with the sucrase purified from the small intestine. Immunodiffusion with antiserum against intestinal sucrase and characterization of the activity revealed that the two enzymes were almost identical. However, the size of the yolk sac sucrase was found to be slightly smaller than that of the intestinal enzyme by chromatography on Sephadex G-200 and polyacrylamide gel electrophoresis. Immunocytochemical studies showed that the sucrase was located on the free surface of yolk sac endodermal cells, but the sucrase may also be present in the cytoplasm.
D000818:Animals; D002642:Chick Embryo; D002645:Chickens; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D005779:Immunodiffusion; D007422:Intestines / Q000201:enzymology; D013393:Sucrase / Q000032:analysis* / Q000302:isolation & purification; D015017:Yolk Sac / Q000201:enzymology*
D013393:Sucrase