Improved biochemical screening procedure for small clinical laboratories for vero (Shiga-like)-toxin-producing strains of Escherichia coli O157:H7.
Of 327 sorbitol-negative fecal isolates of Escherichia coli, 37 O157:H7 Vero (Shiga-like)-toxin-producing strains were identified. The specificity of the basic sorbitol screen was improved from 11.3 to 33.6% by the exclusion of organisms with negative ornithine and lysine decarboxylase reactions.
D001427:Bacterial Toxins / Q000096:biosynthesis*; D003470:Culture Media; D004926:Escherichia coli / Q000145:classification / Q000378:metabolism*; D005243:Feces / Q000382:microbiology; D006801:Humans; D008239:Lysine / Q000378:metabolism; D009952:Ornithine / Q000378:metabolism; D012703:Serotyping; D022622:Shiga Toxin 1; D013012:Sorbitol / Q000378:metabolism*
D001427:Bacterial Toxins; D003470:Culture Media; D022622:Shiga Toxin 1; D013012:Sorbitol; D009952:Ornithine; D008239:Lysine