The inhibition of 3 beta HSD activity in porcine granulosa cells by 4-MA, a potent 5 alpha-reductase inhibitor.
Addition of 17 beta-N,N,-diethylcarbamoyl-4-methyl-4-aza-5 alpha-androstane-3-one, a potent 5 alpha-reductase inhibitor, to granulosa cell cultures inhibited the FSH-stimulated progesterone synthesis during both the initial 48 h induction period and the subsequent 6h test period in a dose-dependent fashion. Besides being a more potent inhibitor of FSH-stimulated progesterone synthesis than testosterone, 4-MA also synergized with the androgen to inhibit progesterone synthesis. These results indicate that 4-MA has a direct inhibitory action on 3 beta-HSD.
D015096:3-Hydroxysteroid Dehydrogenases / Q000037:antagonists & inhibitors*; D000818:Animals; D001378:Azasteroids / Q000494:pharmacology*; D002478:Cells, Cultured; D042944:Cholestenone 5 alpha-Reductase; D013196:Dihydrotestosterone / Q000031:analogs & derivatives* / Q000494:pharmacology; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D006107:Granulosa Cells / Q000187:drug effects* / Q000378:metabolism; D010088:Oxidoreductases / Q000037:antagonists & inhibitors*; D011374:Progesterone / Q000096:biosynthesis; D013260:Steroids, Heterocyclic / Q000494:pharmacology*; D013552:Swine; D013739:Testosterone / Q000494:pharmacology
D001378:Azasteroids; D013260:Steroids, Heterocyclic; D013196:Dihydrotestosterone; D013739:Testosterone; D011374:Progesterone; C031563:17-N,N-diethylcarbamoyl-4-methyl-4-azaandrostane-3-one; D005640:Follicle Stimulating Hormone; D010088:Oxidoreductases; D015096:3-Hydroxysteroid Dehydrogenases; D042944:Cholestenone 5 alpha-Reductase