title: Periodate oxidation of glycolipids: a borohydride-periodate-Schiff method for ganglioside demonstration in tissue sections.
abstract: Among lipids, gangliosides can be selectively stained with Schiff's reagent if the oxidizing agent (sodium metaperiodate) is sufficiently dilute to exclude all but the readily oxidized sialic acid sugars. A borohydride-periodate-Schiff (BhPS) sequence is recommended as a reliable method, convenient to perform, for the detection of the intraneuronal lipid accumulations in the ganglioside storage disorders.
mesh_terms: D001894:Borohydrides; D001923:Brain Chemistry; D005677:G(M1) Ganglioside; D005732:Gangliosides / Q000032:analysis*; D005733:Gangliosidoses / Q000378:metabolism*; D006651:Histocytochemistry; D006801:Humans; D010084:Oxidation-Reduction; D010503:Periodic Acid-Schiff Reaction; D013116:Spinal Cord / Q000032:analysis*; D013661:Tay-Sachs Disease / Q000378:metabolism
chemical_list: D001894:Borohydrides; D005732:Gangliosides; D005677:G(M1) Ganglioside; C025364:sodium borohydride