Increased concentration of the fast-acting plasminogen activator inhibitor in plasma associated with familial venous thrombosis.
We have earlier demonstrated that in a family with a tendency to recurrent venous thrombosis the release of tissue plasminogen activator (t-PA) activity in blood after stimulation was abnormally low. This observation could be related either to an impaired release of t-PA into the blood stream or to a masking of the released t-PA by a high concentration of PA inhibitor(s). In order to distinguish between these two possibilities the family was reinvestigated using various newer techniques, including an ELISA for t-PA, an assay for quantitation of the fast-acting PA inhibitor and SDS polyacrylamide gel electrophoresis followed by fibrin-enzymography. Hereby the family members were demonstrated to have a high concentration in plasma of the PA inhibitor. After stimulation the release of t-PA into the blood was normal, the t-PA activity, however, was immediately inactivated by complex formation with the fast-acting PA inhibitor.
D000328:Adult; D003250:Constriction; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006023:Glycoproteins / Q000097:blood*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010960:Plasminogen Activators / Q000494:pharmacology; D015849:Plasminogen Inactivators; D013924:Thrombophlebitis / Q000097:blood / Q000235:genetics*; D010959:Tissue Plasminogen Activator / Q000037:antagonists & inhibitors* / Q000097:blood / Q000276:immunology; D014568:Urokinase-Type Plasminogen Activator / Q000494:pharmacology
D006023:Glycoproteins; D015849:Plasminogen Inactivators; D010960:Plasminogen Activators; D010959:Tissue Plasminogen Activator; D014568:Urokinase-Type Plasminogen Activator