RecE-dependent lysogenic induction in the absence of repressor in Bacillus subtilis non-complementing diploids.
The RecE protein of Bacillus subtilis, known to be required for induction of the SOS response and of phi 105 prophage, was shown to be involved in mitomycin C induction of B. subtilis diploid lysogens carrying a silent phi 105 prophage in their unexpressed chromosome. These stable non-complementing diploid lysogens, formed by protoplast fusion and regeneration, did not synthesize repressor, so that the induction observed must have resulted from RecE-dependent activation of the prophage rather than from RecE-dependent inactivation of repressor. Mitomycin C treatment does not induce permanent expression of the silent chromosome, so the activation seems to be temporary, perhaps reflecting the action of an SOS function under RecE control.
D001412:Bacillus subtilis / Q000235:genetics*; D001435:Bacteriophages / Q000254:growth & development*; D004171:Diploidy; D029968:Escherichia coli Proteins; D005090:Exodeoxyribonucleases / Q000235:genetics*; D005838:Genotype; D008242:Lysogeny; D016685:Mitomycin; D008937:Mitomycins / Q000494:pharmacology; D010641:Phenotype; D014775:Virus Activation / Q000187:drug effects
D029968:Escherichia coli Proteins; D008937:Mitomycins; D016685:Mitomycin; D005090:Exodeoxyribonucleases; C038225:recE protein, E coli