Induction of histidine decarboxylase by dexamethasone in mastocytoma P-815 cells.
Dexamethasone at a concentration as low as 10 nM significantly increased both the histamine content and histidine decarboxylase activity of cultured mastocytoma P-815 cells. Both effects were clearly seen using several glucocorticoids, which were as effective as dexamethasone. In contrast to that of histamine, the serotonin level of mastocytoma P-815 cells was decreased by treatment with dexamethasone. The dexamethasone-induced increases in histamine content and histidine decarboxylase activity were completely suppressed by the addition of cycloheximide and actinomycin D. Mastocytoma P-815 cells were found to possess binding sites for [3H]dexamethasone in the cytosol (Kd = 15.7 nM) and the nuclei (Kd = 1.26 nM). These results show that glucocorticoids significantly stimulate de novo synthesis of histidine decarboxylase.
D000818:Animals; D001665:Binding Sites; D002262:Carboxy-Lyases / Q000096:biosynthesis*; D002467:Cell Nucleus / Q000378:metabolism; D002478:Cells, Cultured; D003513:Cycloheximide / Q000494:pharmacology; D003600:Cytosol / Q000378:metabolism; D003609:Dactinomycin / Q000494:pharmacology; D003907:Dexamethasone / Q000378:metabolism / Q000494:pharmacology*; D004790:Enzyme Induction / Q000187:drug effects; D006632:Histamine / Q000096:biosynthesis; D006640:Histidine Decarboxylase / Q000096:biosynthesis*; D012515:Mast-Cell Sarcoma / Q000378:metabolism*; D051379:Mice
D003609:Dactinomycin; D003907:Dexamethasone; D006632:Histamine; D003513:Cycloheximide; D002262:Carboxy-Lyases; D006640:Histidine Decarboxylase