Induction of antiphosphocholine antibodies utilizing lambda light chains in BALB/c mice.
BALB/c mice immunized with 1 or 100 micrograms of phosphocholine (PC)-keyhole-limpet hemocyanin absorbed on aluminum hydroxide gel (alum) produced up to 50% lambda 1-light-chain-containing anti-PC antibodies in their serum. Only 10% lambda 1 antibodies were seen when complete Freund's adjuvant or bentonite were used as the adjuvant or if PC-bovine serum albumin was used with alum. Thus, the induction of large lambda-antibody responses to PC (a response usually dominated by kappa-antibodies) was both adjuvant- and carrier-dependent.
D000276:Adjuvants, Immunologic; D000534:Alum Compounds; D000535:Aluminum / Q000276:immunology; D000818:Animals; D000917:Antibody Formation; D001546:Bentonite / Q000276:immunology; D002794:Choline / Q000031:analogs & derivatives*; D006241:Haptens; D006433:Hemocyanins / Q000276:immunology*; D007146:Immunoglobulin lambda-Chains / Q000276:immunology*; D007700:Kinetics; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology*; D010767:Phosphorylcholine / Q000276:immunology*; D013431:Sulfates / Q000276:immunology
D000276:Adjuvants, Immunologic; D000534:Alum Compounds; D006241:Haptens; D007146:Immunoglobulin lambda-Chains; D013431:Sulfates; D010767:Phosphorylcholine; D001546:Bentonite; C041524:aluminum sulfate; D006433:Hemocyanins; D000535:Aluminum; D002794:Choline