Clinical significance and characteristics of creatine kinase-immunoglobulin complexes in sera from patients with malignant tumors.
Creatine kinase (CK, ATP: creatine N-phosphotransferase, EC 2.7.3.2) and immunoglobulin complexes were detected in 27 of 234 (11.5%) patients with malignant tumors. The complexes electrophoresed between CK-MM and the sample application point. The positive rate was significantly higher than rates in patients with benign diseases, or in healthy adults (p less than 0.001). Primary malignancies of the 27 patients were colon 8, gastric 6, pancreatic 4, liver 5, pulmonary 3 and mammary 1. As 22 of the 27 patients had a recurrence or a metastasis and the mortality rate of the positive group 6 mth after the tests was high (85%), most of the 27 patients had advanced tumors. Thus, the CK-immunoglobulin complexes seem to be a prognosticator of patients with advanced tumors. The classes and types of immunoglobulins in the complexes were IgA lambda, 7; IgA kappa lambda, 5; IgA (types of the light chain were not identified), 4; IgA/G kappa, 1; IgA/G lambda, 3; and IgA/G kappa lambda, 7. The molecular sizes of the complexes were 384,000 +/- 22,000 (mean +/- 1 SD). The results of recombination assays using IgA isolated from the complexes of 4 patients suggested that the complexes in the patients' sera were mitochondrial CK-IgA. Non CK-M subunit activities of the positive group were 58 +/- 40 U/l (mean +/- 1 SD) and they were significantly higher than those of the complex negative group of tumor patients (p less than 0.01). This is probably the first report of detection of mitochondrial CK-IgA complexes.
D000328:Adult; D003402:Creatine Kinase / Q000276:immunology*; D006801:Humans; D007070:Immunoglobulin A / Q000378:metabolism; D007145:Immunoglobulin kappa-Chains / Q000276:immunology; D007146:Immunoglobulin lambda-Chains / Q000276:immunology; D007136:Immunoglobulins / Q000276:immunology*; D008928:Mitochondria / Q000201:enzymology; D008970:Molecular Weight; D009369:Neoplasms / Q000276:immunology*; D011379:Prognosis
D007070:Immunoglobulin A; D007145:Immunoglobulin kappa-Chains; D007146:Immunoglobulin lambda-Chains; D007136:Immunoglobulins; D003402:Creatine Kinase