[Bioluminescent methods of analysis in microbiology].
The prospects for application of bioluminescent ATP-metry in microbiology are considered. A bioluminescent assay is proposed to analyse biomass by measuring the content of intracellular ATP by means of immobilized firefly luciferase after ATP extraction with dimethylsulfoxide. The assay can be used for plotting the growth curves of microorganisms and for determining the sensitivity of microorganisms to antibiotics. The detection limit of the assay is 700 cells per ml of the measured solution.
D001412:Bacillus subtilis / Q000302:isolation & purification; D004926:Escherichia coli / Q000302:isolation & purification; D008156:Luciferases; D008163:Luminescent Measurements; D008828:Microbiological Techniques; D012441:Saccharomyces cerevisiae / Q000302:isolation & purification
D008156:Luciferases
Bioliuminestsentnye metody analiza v mikrobiologii.