Integrated biological-physicochemical system for the identification of antitumor compounds in fermentation broths.
A rapid, integrated biological-physicochemical system for the identification of six major classes of antitumor compounds in fermentation broths is described. The system relies upon preliminary fractionation of the fermentation broth by liquid-solid extraction, gradient high-performance liquid chromatography with diode-array spectrophotometric detection of the compounds and automated bioassay. The previously stored UV-VIS spectra of the biologically active peaks are used for identification. Confirmation of compound identity is by thermospray liquid chromatography-mass spectrometry. The method has been applied to representatives of the bleomycin, streptonigrin, echinomycin, chromomycin, actinomycin and anthracycline groups.
D000903:Antibiotics, Antineoplastic / Q000302:isolation & purification*; D001412:Bacillus subtilis / Q000187:drug effects; D001681:Biological Assay; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media; D005285:Fermentation; D013058:Mass Spectrometry; D013056:Spectrophotometry, Ultraviolet
D000903:Antibiotics, Antineoplastic; D003470:Culture Media