title: A GTP-binding protein of Escherichia coli has homology to yeast RAS proteins.
abstract: The DNA sequence of a gene (era) located immediately downstream of the gene (rnc) encoding ribonuclease III of Escherichia coli was determined and found to encode a protein of 316 amino acid residues. The amino acid sequence of this protein, Era, has significant similarity to the yeast RAS proteins. Overexpression of the Era protein was achieved and GTP cross-linking experiments demonstrated that the protein was indeed capable of binding GTP, as are the yeast and mammalian ras gene products. These data indicate that ras-related sequences occur not only in eukaryotes but also in prokaryotes.
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D004926:Escherichia coli / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics*; D019204:GTP-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D008040:Genetic Linkage; D006160:Guanosine Triphosphate / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000235:genetics; D012260:Ribonucleases / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D005656:Fungal Proteins; D011518:Proto-Oncogene Proteins; D006160:Guanosine Triphosphate; D012260:Ribonucleases; D019204:GTP-Binding Proteins