Characterization and diagnostic application of a lipopolysaccharide core oligosaccharide-protein conjugate.
Shigella sonnei phase II core oligosaccharide (OSPhII) was linked covalently with protein (bovine serum albumin or tetanus toxoid) to obtain a conjugate (OSPhII-protein) of good immunogenicity in rabbits. Anti-OSPhII-protein conjugate sera contained high levels of immunoglobulin G antibodies against the complete core region of Sh. sonnei lipopolysaccharide. The antigenic relationships between lipopolysaccharides of R1, R3 and R4 core types using anti-OSPhII-protein sera were studied. These antisera may be used for the rapid determination of core types in unknown enterobacterial strains.
D000818:Animals; D006461:Hemolysis; D007118:Immunoassay / Q000379:methods; D005779:Immunodiffusion / Q000379:methods; D007123:Immunoelectrophoresis, Two-Dimensional / Q000379:methods; D007202:Indicators and Reagents; D008070:Lipopolysaccharides / Q000276:immunology*; D009844:Oligosaccharides / Q000276:immunology; D011817:Rabbits / Q000276:immunology; D012710:Serum Albumin, Bovine; D012763:Shigella flexneri / Q000276:immunology; D012764:Shigella sonnei / Q000276:immunology; D013745:Tetanus Toxoid
D007202:Indicators and Reagents; D008070:Lipopolysaccharides; D009844:Oligosaccharides; D013745:Tetanus Toxoid; D012710:Serum Albumin, Bovine