Production of 5-formyluracil from thymine in an in vitro active oxygen-generating system.
Thymine was placed in a model active oxygen-generating system containing ferrous sulfate, EDTA, and ascorbic acid. The oxidative products of thymine were separated by Sephadex LH-20 chromatography and a reversed phase high-performance liquid chromatography (HPLC) into at least five major components. One of them had a UV spectrum characteristic of 5-formyluracil and mass spectrometric analysis of this material also indicated this material to be 5-formyluracil.
D001205:Ascorbic Acid / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D004492:Edetic Acid / Q000378:metabolism; D005296:Ferrous Compounds / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet; D013941:Thymine / Q000378:metabolism*; D014498:Uracil / Q000031:analogs & derivatives* / Q000138:chemical synthesis
D005296:Ferrous Compounds; C007777:5-formyluracil; C020748:ferrous sulfate; D014498:Uracil; D004492:Edetic Acid; D001205:Ascorbic Acid; D013941:Thymine; D010100:Oxygen