[Absence of in vivo binding of deoxyribonucleic acid to the glomerular basement membrane in C57BL/6 mice].
The intraaortic injection of radiolabeled deoxyribonucleic acid (DNA) into C57BL/6 mice treated with bacterial lipopolysaccharides 48 hrs. before, induced the renal deposition of DNA, as previously reported. Autoradiographic studies of the kidneys obtained from such mice did not demonstrate a selective binding of radiolabeled DNA to the glomerular basal membrane. These results argue against the in situ formation of DNA:anti-DNA immune complexes which is a proposed mechanism in the development of tissue lesions in the course of systemic lupus erythematosus.
D000818:Animals; D000974:Antibodies, Antinuclear / Q000378:metabolism*; D000936:Antigen-Antibody Complex / Q000378:metabolism*; D001345:Autoradiography; D001485:Basement Membrane / Q000276:immunology; D004247:DNA / Q000276:immunology*; D004277:DNA, Single-Stranded / Q000276:immunology; D005260:Female; D007678:Kidney Glomerulus / Q000276:immunology*; D008180:Lupus Erythematosus, Systemic / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D014316:Tritium
D000974:Antibodies, Antinuclear; D000936:Antigen-Antibody Complex; D004277:DNA, Single-Stranded; D014316:Tritium; D004247:DNA
Absence de fixation in vivo de l'acide désoxyribonucléique sur la membrane basale glomérulaire chez la souris C57BL/6.