The Escherichia coli K-12 SOS chromotest agar spot test for simple, rapid detection of genotoxic agents.
The Escherichia coli K-12 SOS chromotest is a colorimetric (beta-galactosidase induction) system for detecting genotoxic chemicals as agents which induce filamentation in response to DNA damage. The chromotest was modified from a liquid suspension assay to a simple, convenient agar spot test, which was performed in the manner of a related colorimetric prophage induction assay (BIA). Chromotest agar dishes yielded optimal results after 16-18 h incubation, presumably because of the agar growth characteristics of tester strain PQ37. Of 44 tested chemicals, nitro aromatics, cytotoxic/antitumor agents, polycyclic hydrocarbons and aflatoxins showed good activity. Alkylating agents such as MNNG and MMS were active only at high concentrations. Compounds active in both the chromotest and BIA were active at 10-100-fold lower concentrations in the chromotest. The chromotest appeared to be less effective than the Salmonella Ames mutagenicity test in the detection of diverse classes of chemical carcinogens. The chromotest may be a useful alternative to the BIA in the study of particular classes of genotoxic compounds.
D001711:Biotransformation; D003090:Coliphages / Q000254:growth & development; D004260:DNA Repair / Q000187:drug effects*; D004790:Enzyme Induction / Q000187:drug effects; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D009152:Mutagenicity Tests; D009153:Mutagens / Q000378:metabolism; D012486:Salmonella typhimurium / Q000235:genetics; D013329:Structure-Activity Relationship; D014775:Virus Activation / Q000187:drug effects; D001616:beta-Galactosidase / Q000096:biosynthesis
D009153:Mutagens; D001616:beta-Galactosidase