Technetium-99m labeling of murine monoclonal antibody fragments.
F(ab')2 fragments of several murine monoclonal antibodies have been labeled with 99mTc by a direct, pretinning method. The fragments were incubated with stannous ions overnight to split disulfide groups--a process which converts dimeric F(ab')2 to monomeric fragments. The pretinned fragments were then either directly labeled with 99mTc, frozen for subsequent labeling, or lyophilized to make kits for 99mTc-labeling at some later date. The 99mTc-labeled fragments were shown to be stable against transchelation when challenged with ethylenediaminetetraacetic acid, retained immunoreactivity, and were capable of binding to human tumor xenografts in nude mice.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis; D002460:Cell Line; D002614:Chelating Agents; D006063:Chorionic Gonadotropin / Q000276:immunology; D004492:Edetic Acid; D005260:Female; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000032:analysis / Q000276:immunology*; D007553:Isotope Labeling; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D009369:Neoplasms / Q000175:diagnosis* / Q000000981:diagnostic imaging; D011786:Quality Control; D011877:Radionuclide Imaging; D013667:Technetium; D014018:Tissue Distribution; D014183:Transplantation, Heterologous
D000911:Antibodies, Monoclonal; D002614:Chelating Agents; D006063:Chorionic Gonadotropin; D007140:Immunoglobulin Fab Fragments; D013667:Technetium; D004492:Edetic Acid