Antagonistic regulatory properties of the Fc region of immunoglobulin.
Coculture of human peripheral blood mononuclear cells with Fc fragments of human IgG, or the synthetic Fc region-derived peptide, p23, results in the release of oxidative products of arachidonic acid. Prostaglandin E was the major arachidonic acid metabolite found in the culture supernatants. Induction of polyclonal antibody production by Fc fragments and p23 is influenced by the concomitant production of prostaglandin E in culture. Addition of prostaglandin synthetase inhibitors, indomethacin and aspirin, to human peripheral blood mononuclear cell cultures resulted in a significant increase in the amount of polyclonal antibody produced. Moreover, addition of exogenous prostaglandin E to these cultures abrogated the ability of indomethacin to enhance Fc fragment-induced polyclonal antibody production. These results suggest that Fc fragments possess bifunctional immunoregulatory properties.
D000328:Adult; D000917:Antibody Formation / Q000187:drug effects; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000097:blood*; D002448:Cell Adhesion; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D015232:Dinoprostone; D005260:Female; D006801:Humans; D007140:Immunoglobulin Fab Fragments; D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G; D007700:Kinetics; D008297:Male; D009000:Monocytes / Q000166:cytology / Q000276:immunology*; D010084:Oxidation-Reduction; D011458:Prostaglandins E / Q000097:blood* / Q000494:pharmacology; D011863:Radioimmunoassay
D001095:Arachidonic Acids; D007140:Immunoglobulin Fab Fragments; D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G; D011458:Prostaglandins E; D016718:Arachidonic Acid; D015232:Dinoprostone