title: Effect of growth temperature on the lipids, outer membrane proteins, and lipopolysaccharides of Pseudomonas aeruginosa PAO.
abstract: Growth of Pseudomonas aeruginosa PAO1 at 15 to 45 degrees C in tryptic soy broth resulted in changes in the lipids, lipopolysaccharides (LPSs), and outer membrane proteins of the cells. Cells grown at 15 degrees C contained, relative to those cultivated at 45 degrees C, increased levels of the phospholipid fatty acids hexadecenoate and octadecenoate and reduced levels of the corresponding saturated fatty acids. Furthermore, the lipid A fatty acids also showed thermoadaptation with decreases in dodecanoic and hexadecanoic acids and increases in the level of 3-hydroxydecanoate and 2-hydroxdodecanoate as the growth temperature decreased. In addition, LPS extracted from cells cultivated at the lower temperatures contained a higher content of long-chain S-form molecules than that isolated from cells grown at higher temperatures. On the other hand, the percentage of LPS cores substituted with side-chain material decreased from 37.6 mol% at 45 degrees C to 19.3 mol% at 15 degrees C. The outer membrane protein profiles indicated that at low growth temperatures there was an increase in a polypeptide with an apparent molecular weight of 43,000 and decreases in the content of 21,000 (protein H1)- and 27,500-molecular-weight proteins.
mesh_terms: D000222:Adaptation, Physiological; D001425:Bacterial Outer Membrane Proteins / Q000502:physiology; D005227:Fatty Acids / Q000378:metabolism; D008070:Lipopolysaccharides / Q000502:physiology; D008563:Membrane Lipids / Q000502:physiology; D008970:Molecular Weight; D011550:Pseudomonas aeruginosa / Q000254:growth & development / Q000502:physiology*; D012995:Solubility; D013696:Temperature
chemical_list: D001425:Bacterial Outer Membrane Proteins; D005227:Fatty Acids; D008070:Lipopolysaccharides; D008563:Membrane Lipids