title: Identification of two glycoproteins of chromaffin granules as the carboxypeptidase H.
abstract: An antiserum against carboxypeptidase H, an enzyme involved in the biosynthesis of neuropeptides such as the enkephalins, was used to identify the enzyme proteins in bovine chromaffin granules. Two-dimensional immuno- and lectin-blots revealed that two closely migrating glycoproteins which have been previously named J and K represented the enzyme. The same protein doublet was present in the membrane preparation and the soluble lysate of bovine chromaffin granules; however, the membrane contained significantly more enzyme protein.
mesh_terms: D000818:Animals; D043423:Carboxypeptidase H; D002268:Carboxypeptidases / Q000032:analysis*; D002417:Cattle; D002837:Chromaffin Granules / Q000032:analysis / Q000201:enzymology*; D002838:Chromaffin System / Q000201:enzymology*; D003600:Cytosol / Q000201:enzymology; D006023:Glycoproteins / Q000032:analysis*; D008565:Membrane Proteins / Q000032:analysis
chemical_list: D006023:Glycoproteins; D008565:Membrane Proteins; D002268:Carboxypeptidases; D043423:Carboxypeptidase H