A rapid method for detection of tyrosinase activity in electrophoresis.
This rapid and sensitive method for localizing tyrosinase in polyacrylamide slab gels is based on the condensation of Bestthorn's hydrazone (3 methyl-2-benzothiazolinone hydrazone hydrochloride) with the quinone obtained by enzymatic oxidation of phenol. Both monophenolase and diphenolase activities are localized by this method.
D000818:Animals; D052160:Benzothiazoles; D004156:Catechol Oxidase / Q000032:analysis*; D002396:Catechols; D003445:Crustacea; D004591:Electrophoresis, Polyacrylamide Gel; D006835:Hydrazones; D014442:Monophenol Monooxygenase / Q000032:analysis*; D013844:Thiazoles
D052160:Benzothiazoles; D002396:Catechols; D006835:Hydrazones; D013844:Thiazoles; D004156:Catechol Oxidase; D014442:Monophenol Monooxygenase; C002536:3-methyl-2-benzothiazolone hydrazone