[Balance of macroergic compounds during the growth of Thiobacillus ferrooxidans].
The balance of energy-rich compounds (ERC) was drawn up for the growth of Thiobacillus ferrooxidans in a medium with ferrous ions as an energy source. The balance items and the phosphorylating efficiency of oxidation (P/2e-) were calculated basing on the experimental yield values using the ERC balance equation. At a specific growth rate of 0.1 h-1, 55% of ferrous ions are used for the synthesis of cell biomass, 7.5% for maintainance, 4% of the ions are oxidized to reduce NAD+, and 34% are used to produce ERC necessary for the reduction. Here, 24% of ERC are used for the synthesis of monomers from CO2, 42% for the production of NADH, 24% for the biomass synthesis from monomers, and 10% for maintaining cell activity. The P/2e- for the oxidation of ferrous ions is 0.19 mole of ERC per 2e-. This is possible only when the [Fe3+]/[Fe2+] ratio in the cell periplasm is 1 X 10(3)-1 X 10(4).
D002245:Carbon Dioxide / Q000378:metabolism; D003470:Culture Media / Q000378:metabolism; D004579:Electron Transport; D004734:Energy Metabolism; D007501:Iron / Q000378:metabolism; D008433:Mathematics; D010084:Oxidation-Reduction; D013855:Thiobacillus / Q000254:growth & development / Q000378:metabolism*
D003470:Culture Media; D002245:Carbon Dioxide; D007501:Iron
Balans makroérgicheskikh soedineniĭ pri roste Thiobacillus ferrooxidans.