[Directions of alkylation of deoxyguanosine and deoxyguanylic acid by thio-TEPA].
As exemplified with the reaction of well-known anticancer agent thio-TEPA with dGMP, the ability of ethylenimine derivatives to modify this DNA component under the conditions approaching the biological ones was demonstrated for the first time. Modification involves mainly the phosphate group alkylation. In addition, thio-TEPA alkylates the base in dGMP at position 7. The formation of dGMP 7-alkyl derivatives was confirmed by detecting the luminescent products generated in the course of this reaction.
D000478:Alkylation; D055598:Chemical Phenomena; D002621:Chemistry; D002848:Chromatography, DEAE-Cellulose; D002851:Chromatography, High Pressure Liquid; D003848:Deoxyguanine Nucleotides / Q000032:analysis*; D003849:Deoxyguanosine / Q000032:analysis*; D066298:In Vitro Techniques; D013056:Spectrophotometry, Ultraviolet; D013852:Thiotepa / Q000494:pharmacology*
D003848:Deoxyguanine Nucleotides; C007257:2'-deoxyguanosine 5'-phosphate; D013852:Thiotepa; D003849:Deoxyguanosine
Napravleniia alkilirovaniia dezoksiguanozina i dezoksiguanilovoĭ kisloty tiotéfom.