New support for the large-scale purification of proteins.
We propose a new affinity sorbent, matrix-linked histidine, for the large-scale purification of proteins. A variety of proteins and certain peptides, each distinct from the other, were purified. Immunoglobulin G from human placenta was chosen for a detailed study concerning the effects of coupling, spacer-arm and other parameters. Multiple interactions such as charge-transfer and other ionic reactions have been suggested to be responsible for the interactions between proteins and ligand.
D000818:Animals; D002268:Carboxypeptidases / Q000302:isolation & purification; D043402:Cathepsin A; D002417:Cattle; D002642:Chick Embryo; D012085:Chymosin / Q000302:isolation & purification; D010450:Endopeptidases / Q000302:isolation & purification; D005260:Female; D006639:Histidine / Q000302:isolation & purification; D006801:Humans; D007122:Immunoelectrophoresis; D007074:Immunoglobulin G / Q000302:isolation & purification; D010455:Peptides / Q000302:isolation & purification; D010920:Placenta / Q000032:analysis; D011247:Pregnancy; D011506:Proteins / Q000302:isolation & purification*; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D029701:Saccharomyces cerevisiae Proteins; D012685:Sepharose / Q000031:analogs & derivatives / Q000302:isolation & purification; D012697:Serine Endopeptidases; D013056:Spectrophotometry, Ultraviolet
D007074:Immunoglobulin G; D010455:Peptides; D011506:Proteins; D029701:Saccharomyces cerevisiae Proteins; C044116:histidyl-sepharose; D006639:Histidine; D012685:Sepharose; D002268:Carboxypeptidases; D010450:Endopeptidases; D043402:Cathepsin A; C499283:PRC1 protein, S cerevisiae; C022633:serine carboxypeptidase; D012697:Serine Endopeptidases; C026109:oryzin; D012085:Chymosin