Significance of transported glycine in the conjugation of sodium benzoate in spf mutant mice with ornithine transcarbamylase deficiency.
3H-glycine and 14C-serine were injected intraperitoneally, during treatment of spf mutant mice with 2% sodium benzoate in drinking water. Urinary hippurate was separated by thin layer chromatography and counted for 3H and 14C labels representing transported and newly synthesized glycine, respectively. The specific activity of 3H-hippurate increased significantly in mutant and normal groups, while the increase of 14C was seen only in mutants. The ratio of specific activity 3H:14C showed significant increases in normal (0.99 to 1.93; p less than 0.01) and mutant (1.53 to 3.05; p less than 0.05) groups, which shows that glycine transported from body pools played a significantly greater role in the conjugation of benzoate, compared to glycine synthesized de novo from serine. In spf mice, benzoate treatment also resulted in a decrease in orotate excretion, indicating amelioration of the hyperammonemic state. It is postulated that the elimination of glycine transported from body pools may be the primary mechanism for the reduction of ammoniagenicity in benzoate therapy, and that the de novo synthesis of glycine may have a secondary effect.
D000818:Animals; D001565:Benzoates / Q000378:metabolism*; D019817:Benzoic Acid; D002250:Carbon Radioisotopes; D005998:Glycine / Q000378:metabolism*; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D051379:Mice; D008817:Mice, Mutant Strains; D009154:Mutation; D020163:Ornithine Carbamoyltransferase Deficiency Disease; D012694:Serine / Q000378:metabolism; D013045:Species Specificity; D014316:Tritium
D001565:Benzoates; D002250:Carbon Radioisotopes; D014316:Tritium; D012694:Serine; D019817:Benzoic Acid; D005998:Glycine