The Drosophila homolog of the mouse mammary oncogene int-1 is identical to the segment polarity gene wingless.
We have isolated the Drosophila melanogaster homolog (Dint-1) of int-1, a conserved cellular oncogene implicated in viral mammary tumorigenesis in mice. The deduced Dint-1 protein sequence contains 468 amino acids and starts with a hydrophobic leader; it is 54% identical to the int-1 sequence, and all 23 cysteine residues are conserved. The putative Drosophila protein has an extra sequence of 85 amino acids, encoded on an additional exon. Dint-1 is expressed throughout development, but transcripts are barely detectable in adult flies. Hybridization in situ to embryos reveals a segmented pattern of expression. We show that Dint-1 and the segment polarity gene wingless are identical and map to the same location. The sequence of the gene suggests that the Dint-1/wingless protein functions in morphogenesis as a signal in cell-cell communication.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000235:genetics; D004331:Drosophila melanogaster / Q000196:embryology / Q000235:genetics*; D005786:Gene Expression Regulation; D005796:Genes; D007301:Insect Hormones / Q000235:genetics* / Q000502:physiology; D007814:Larva / Q000032:analysis / Q000648:ultrastructure; D008325:Mammary Neoplasms, Experimental / Q000235:genetics; D051379:Mice / Q000196:embryology / Q000235:genetics; D009024:Morphogenesis; D011518:Proto-Oncogene Proteins / Q000235:genetics; D011519:Proto-Oncogenes; D012689:Sequence Homology, Nucleic Acid; D051153:Wnt Proteins; D051155:Wnt1 Protein; D029961:Zebrafish Proteins
D007301:Insect Hormones; D011518:Proto-Oncogene Proteins; D051153:Wnt Proteins; D051155:Wnt1 Protein; D029961:Zebrafish Proteins; D004247:DNA