Molecular characterization of Ro(SSA) and La(SSB) proteins.
Ro(SSA) and La(SSB) proteins were characterized using human spleen extract as antigen and monospecific anti-Ro(SSA) and anti-La(SSB) sera. The antigens were partially purified by DEAE cellulose and Sephacryl chromatography, and then were electrotransferred to nitrocellulose sheets after SDS-PAGE electrophoresis. The anti-Ro(SSA) sera had reacted with a polypeptidic band of 58 kilodaltons (kDa), meanwhile the La(SSB) antisera reacted with 2 polypeptidic bands of 42 and 40 kDa. The molecular weight of the Ro(SSA) and La(SSB) proteins obtained by gel filtration chromatography was in agreement with the PAGE/electrotransfer results. These procedures are useful tools for the molecular analysis of the markers in the connective tissue diseases.
D000941:Antigens / Q000302:isolation & purification*; D001324:Autoantigens / Q000276:immunology / Q000302:isolation & purification*; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D005779:Immunodiffusion; D020733:RNA, Small Cytoplasmic; D012261:Ribonucleoproteins; D013154:Spleen / Q000032:analysis; D000097585:SS-B Antigen; D000099207:SS-A Antigen
D000941:Antigens; D001324:Autoantigens; D020733:RNA, Small Cytoplasmic; D012261:Ribonucleoproteins; D000097585:SS-B Antigen; D000099207:SS-A Antigen; C116006:RO60 protein, human