Occurrence and aeruginocine typing of Pseudomonas aeruginosa in buffaloes and their environment.
The occurrence and distribution of Pseudomonas aeruginosa in buffaloes and their environment was studied. Environmental sources included milkers, milking utensils, watering troughs, animal shed floor, barn-yard soil and drains. Of the different body sites examined, the organism could only be isolated from 15% of the muzzle and 5% of the belly samples. The organism was widely prevalent in the animal environment and could be isolated from milking utensils (56.67%), watering troughs (44.00%), drains (36.37%), shed floor (4.00%), barnyard soil (3.33%), and milkers' throats (50.00%) and hands before and after milking (7.14 and 10.71%, respectively). Aeruginocine typing revealed that of the 68 strains of P. aeruginosa, 65 (95.59%) were typable. Amongst the typable strains, 21 (32.30%) were classifiable and 44 (67.69%) unclassifiable. Unclassifiable pattern 23478- was most predominant and common to animals and environmental sources. Different aeruginocine types encountered were: A(2), B(4), F(1), L(1), P(4), 1(1), 8(1), 9(1), 11(3), 12(1), 14(1) and 17(1). This is of significance as most of the types encountered have been reported from clinical specimens of animal and human origin.
D000818:Animals; D002020:Buffaloes / Q000382:microbiology*; D005260:Female; D006801:Humans; D007774:Lactation; D008892:Milk / Q000382:microbiology; D011247:Pregnancy; D011550:Pseudomonas aeruginosa / Q000145:classification / Q000302:isolation & purification*; D012867:Skin / Q000382:microbiology