The relationship between benzo(a)pyrene diol-epoxide-DNA adducts and mutagenicity in the CHO/HPGRT assay.
We have studied the relationship between DNA adducts in Chinese hamster ovary (CHO) cells and mutagenicity as determined in the CHO/hypoxanthine-guanine phosphoribosyltransferase assay. The cells were treated with benzo(a)pyrene 7,8-diol (BP-diol) in the presence of a bioactivation system, S9 mix. DNA binding by bioactivation of BP-diol with S9 mix occurred with both stereoisomers of benzo(a)pyrene diol-epoxide (BPDE) in approximately equal amounts. The number of BPDE-DNA adducts (21-260 adducts/10(6) nucleotide base pairs) increased with increasing treatment concentrations of BP-diol (1.4-7.0 microM). A linear relationship was observed between the number of BPDE-DNA adducts and mutagenicity (89-605 mutants/10(6) cloneable cells) over the concentration range of BP-diol assayed.
D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide / Q000378:metabolism* / Q000633:toxicity*; D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000378:metabolism*; D018736:DNA Adducts; D004101:Dihydroxydihydrobenzopyrenes / Q000378:metabolism* / Q000633:toxicity*; D005260:Female; D007041:Hypoxanthine Phosphoribosyltransferase / Q000378:metabolism*; D007700:Kinetics; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D009154:Mutation; D010053:Ovary
D018736:DNA Adducts; D004101:Dihydroxydihydrobenzopyrenes; D009153:Mutagens; C043931:benzo(a)pyrene-7,8-dihydrodiol-9,10-epoxide-DNA; D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide; D004247:DNA; D007041:Hypoxanthine Phosphoribosyltransferase