[Biochemical and immunochemical study of antigen preparations of Rickettsia prowazekii].
The biochemical and immunochemical study of the qualitative composition of R. prowazekii antigenic preparations, isolated and purified by different methods, indicates that these preparations contain high-molecular polypeptide 3 (133600 D), morphologically linked with the cell membrane of R. prowazekii. The method of adsorption chromatography on calcium phosphate permits obtaining specific rickettsial antigens with a greater degree of purification from ballast admixtures than the methods of acid precipitation and gel filtration on Sephadex G-200.
D000327:Adsorption; D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology* / Q000302:isolation & purification; D000954:Antigens, Surface / Q000276:immunology / Q000378:metabolism; D002462:Cell Membrane / Q000276:immunology; D002642:Chick Embryo; D002845:Chromatography / Q000379:methods; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D007120:Immunochemistry; D005779:Immunodiffusion; D007123:Immunoelectrophoresis, Two-Dimensional; D008970:Molecular Weight; D010455:Peptides / Q000276:immunology / Q000378:metabolism; D012283:Rickettsia prowazekii / Q000276:immunology*; D012995:Solubility
D000942:Antigens, Bacterial; D000954:Antigens, Surface; D010455:Peptides
Biokhimicheskoe i immunokhimicheskoe izuchenie antigennykh preparatov rikettsiĭ Provacheka.