Ovine ureteral motility as a new assay for the cyclo-oxygenase blocking potency of non steroidal anti-inflammatory drugs.
Isolated sheep ureteral ring suspended in an organ bath exhibits rhythmic contractions which are dose-dependently inhibited by indomethacin and revived by prostaglandin E2 or F2 alpha. Seven non-steroidal anti-inflammatory drugs (NSAID) belonging to different chemical groups were tested on the isolated ureteral ring model in molar concentrations of 10(-8)-10(-4) to determine the time elapsing from application of test drug to complete inhibition of ureteral contraction ("stop-time"). The descending order of potency of the NSAID was: flurbiprofen greater than indomethacin greater than flufenamic acid greater than tolmetin greater than aspirin greater than phenylbutazone greater than isoxicam. A parallelism was observed in the plot of log concentration of NSAID and "stop-time" amongst all compounds except aspirin and isoxicam. The sheep isolated ring preparation is a simple reproducible biological model for assessment of anti-inflammatory cyclo-oxygenase inhibitory activity of compounds.
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D016861:Cyclooxygenase Inhibitors; D015237:Dinoprost; D015232:Dinoprostone; D005260:Female; D066298:In Vitro Techniques; D007213:Indomethacin / Q000494:pharmacology; D011458:Prostaglandins E / Q000494:pharmacology; D011460:Prostaglandins F / Q000494:pharmacology; D012756:Sheep; D014590:Uterine Contraction / Q000187:drug effects*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D016861:Cyclooxygenase Inhibitors; D011458:Prostaglandins E; D011460:Prostaglandins F; D015237:Dinoprost; D015232:Dinoprostone; D007213:Indomethacin