Biochemical and immunological studies of bovine and porcine neurofilament triplet proteins by peptide mapping after cyanogen bromide cleavage.
Peptide mapping of the three bovine and porcine neurofilament protein subunits ("L", "M" and "H") with apparent mol. wts of 70, 160 and 210 kDa were performed with CNBr, leading to the cleavage of methionyl bonds. We have obtained two characteristic large fragments with molecular weights of 85 kDa for the "M" bovine subunit and 135 kDa for the "H" subunit of bovine neurofilament. A comparison of the electrophoretic patterns of CNBr generated polypeptides of "L" subunit from beef and pig showed that they are highly related structures. The peptide mappings of CNBr peptides of "M" and "H" subunits from beef and pig were significantly different. Antibodies were raised against the 85 kDa and 135 kDa CNBr fragments. Immunoblotting results with anti-85 kDa and anti-135 kDa of beef are in favour of large differences of structure between the "M" subunits from pig and beef. The "H" proteins were very similar and they also showed that the C-terminal part of bovine "H" and "M" proteins share common antigenic determinants.
D000818:Animals; D000936:Antigen-Antibody Complex; D002417:Cattle; D003488:Cyanogen Bromide; D007106:Immune Sera; D007381:Intermediate Filament Proteins / Q000302:isolation & purification*; D046911:Macromolecular Substances; D008970:Molecular Weight; D016900:Neurofilament Proteins; D010449:Peptide Mapping; D013045:Species Specificity; D013552:Swine
D000936:Antigen-Antibody Complex; D007106:Immune Sera; D007381:Intermediate Filament Proteins; D046911:Macromolecular Substances; D016900:Neurofilament Proteins; D003488:Cyanogen Bromide