Regulation of murine class I genes by interferons is controlled by regions located both 5' and 3' to the transcription initiation site.
Interferons regulate the expression of a large number of mammalian genes, including the major histocompatibility antigen genes. To investigate the mechanisms involved in interferon action, we have analyzed the ability of murine H-2Ld and H-2Dd DNA sequences to control the responses to interferon. The results indicate that interferon regulation of class I gene expression is complex and involves at least two mechanisms that are dependent on class I sequences located upstream and downstream to the transcription initiation site. In transfected mouse L cells, both of these regions are required for full enhancement of class I gene expression, with the major portion of the response controlled by the sequences located 3' to the transcription initiation site. The fine-mapping analysis of the 5' region-encoded response also suggests that recombinant alpha and gamma interferons may exert their effects on class I gene expression by using different cis-acting regulatory sequences.
D000818:Animals; D001483:Base Sequence; D003360:Cosmids; D005786:Gene Expression Regulation / Q000187:drug effects*; D005796:Genes / Q000187:drug effects*; D006183:H-2 Antigens / Q000235:genetics*; D006680:HLA Antigens / Q000235:genetics*; D007370:Interferon Type I / Q000494:pharmacology*; D007371:Interferon-gamma / Q000494:pharmacology*; D007739:L Cells / Q000276:immunology; D008285:Major Histocompatibility Complex / Q000187:drug effects*; D051379:Mice; D008807:Mice, Inbred BALB C; D011994:Recombinant Proteins / Q000494:pharmacology*; D014158:Transcription, Genetic / Q000187:drug effects*
D006183:H-2 Antigens; D006680:HLA Antigens; D007370:Interferon Type I; D011994:Recombinant Proteins; D007371:Interferon-gamma