A phospholipase A2 isoenzyme provokes lipoxin B formation from endogenous sources of arachidonic acid in porcine leukocytes.
Porcine leukocytes incubated with an isoenzyme of phospholipase A2 (PLA2) (isolated from snake venom) produced several trihydroxytetraene- containing compounds which were derived from endogenous sources of arachidonic acid. The formation of these compounds was dose-dependent with an EC50 of approximately 1.25 X 10(-8) M. At this concentration of the isoenzyme and time of exposure the cells remained viable as determined by the exclusion of trypan blue. The compounds were purified by HPLC and their identities were determined by physical criteria which included U.V. spectrometry, GC/MS and by comparison with both synthetic and authentic materials. The biologically derived compounds proved to be lipoxin B (5S, 14R, 15S-trihydroxy-6, 10, 12-trans-8-cis-eicosatetraenoic acid) and its two structural isomers (8-trans-LXB and 14S-8-trans-LXB). Of interest, only small amounts of lipoxin A and its isomers were found in these incubations. Results of the present study indicate that porcine leukocytes can generate lipoxin B and its isomers from endogenous sources of arachidonic acid. Moreover, they suggest that certain PLA2 isoenzymes may initiate the formation of lipoxins and related compounds.
D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000097:blood; D006893:Hydroxyeicosatetraenoic Acids / Q000097:blood* / Q000378:metabolism; D066298:In Vitro Techniques; D007527:Isoenzymes / Q000494:pharmacology*; D007962:Leukocytes / Q000187:drug effects / Q000378:metabolism*; D044045:Lipoxins; D010740:Phospholipases / Q000494:pharmacology*; D010741:Phospholipases A / Q000494:pharmacology*; D054467:Phospholipases A2; D013552:Swine
D001095:Arachidonic Acids; D006893:Hydroxyeicosatetraenoic Acids; D007527:Isoenzymes; D044045:Lipoxins; D016718:Arachidonic Acid; C025984:15-hydroxy-5,8,11,13-eicosatetraenoic acid; C042651:lipoxin B4; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2