Selective regeneration of Bacillus subtilis protoplasts transformed to kanamycin resistance.
An HTY medium osmotically stabilized with 0.5 M D-glucitol was used for regeneration of Bacillus subtilis protoplasts. The application of glucitol as osmotic stabilizer allows simultaneous selection of cells resistant to kanamycin to be made since this antibiotic is not inactivated by glucitol when added to the regeneration medium.
D001412:Bacillus subtilis / Q000187:drug effects / Q000235:genetics / Q000502:physiology* / Q000648:ultrastructure; D003470:Culture Media; D007612:Kanamycin / Q000494:pharmacology*; D009994:Osmolar Concentration; D011523:Protoplasts / Q000187:drug effects / Q000502:physiology*; D011815:R Factors; D013012:Sorbitol; D014169:Transformation, Bacterial
D003470:Culture Media; D013012:Sorbitol; D007612:Kanamycin