Gamma interferon-mediated increase in the number of Ia-bearing macrophages during infection with Listeria monocytogenes.
The role of gamma interferon (IFN-gamma) in an increase in Ia-bearing macrophages during Listeria monocytogenes infection was studied. The peritoneal macrophages from L. monocytogenes-infected mice contained a high proportion of Ia. Intraperitoneal injection of the supernatant from a culture of spleen cells from L. monocytogenes-infected mice induced Ia-rich exudates in normal mice. The Ia-inducing activity in the culture supernatant was abrogated by the pretreatment of spleen cells with anti-Thy-1.2 antibody plus complement. Immunoadsorption of the culture supernatant with anti-recombinant IFN-gamma antibody and protein A-Sepharose CL-4B completely abrogated its Ia-inducing activity. These results suggested that an increase in Ia-bearing macrophages during L. monocytogenes infection was attributable to T-cell-derived IFN-gamma.
D000818:Animals; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D007371:Interferon-gamma / Q000502:physiology*; D008088:Listeriosis / Q000276:immunology*; D008222:Lymphokines / Q000502:physiology; D008262:Macrophage Activation; D016215:Macrophage-Activating Factors; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D013601:T-Lymphocytes / Q000276:immunology; D013997:Time Factors
D000949:Histocompatibility Antigens Class II; D008222:Lymphokines; D016215:Macrophage-Activating Factors; D007371:Interferon-gamma