title: Induction of ovulation in the postpartum rhesus monkey: factors determining success in obtaining primate luteal tissue.
abstract: Ovulation induction in the postpartum rhesus monkey was attempted with the use of purified human pituitary gonadotropins for assessment of (1) whether this procedure could be used to provide a source of luteal tissue; (2) the extent of ovarian responsiveness to gonadotropic stimulation; (3) factors that might facilitate ovulation induction during the peurperium; and (4) factors that might be a contributory cause of induction failure. Twenty rhesus monkeys were treated with purified human follicle-stimulating hormone (hFSH) and human luteinizing hormone (hLH) twice daily, beginning on days 0, 10, or 20 postpartum, with or without prior administration of bromocriptine. Laparotomies were performed during the midluteal phase. Ovaries were examined, and all corpora lutea were removed. Neither the day of beginning the gonadotropin treatment nor bromocriptine administration had a significant effect on the success rate of ovulation induction, which averaged 60% overall. Inductions begun during July had a significantly (P less than 0.025) lower success rate than those started at other times of the year. Antibodies to hFSH and hLH were detected in serum from monkeys that had undergone ovulation induction. Antibodies to hFSH, but not hLH, were associated with significantly reduced induction success (P less than 0.05). Plasma estradiol rose in response to gonadotropin treatment, and the induced follicular phase averaged 13.6 +/- 0.7 days. In all animals judged to have ovulated, corpora lutea were observed at laparotomy, and plasma concentrations of progesterone were significantly elevated (13.8 +/- 3.8 ng/ml).(ABSTRACT TRUNCATED AT 250 WORDS)
mesh_terms: D000818:Animals; D000906:Antibodies / Q000032:analysis; D001971:Bromocriptine / Q000494:pharmacology; D003338:Corpus Luteum; D005260:Female; D005640:Follicle Stimulating Hormone / Q000276:immunology / Q000494:pharmacology; D005498:Follicular Phase; D006801:Humans; D008183:Luteal Phase; D007986:Luteinizing Hormone / Q000276:immunology / Q000494:pharmacology; D008251:Macaca / Q000502:physiology*; D008253:Macaca mulatta / Q000502:physiology*; D010060:Ovulation; D010062:Ovulation Induction; D049590:Postpartum Period; D011247:Pregnancy; D012621:Seasons
chemical_list: D000906:Antibodies; D001971:Bromocriptine; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone