Rapid release and deactivation of plasminogen activators in human endothelial cell cultures in the presence of thrombin and ionophore A23187.
alpha-Thrombin, DFP-thrombin, and ionophore A23187 induce the rapid release (less than 5 minutes) of a variety of proteins, including t-PA forms (Mr 110 and 70 k, after SDS-PAGE) from primary cultures, and both t-PA and u-PA (Mr 90 and 54 k) from subcultured human HUVECs. All PA activity forms are rapidly decreased in the releasates by some unknown mechanism. gamma-Thrombin does not induce the release of PAs from cultured HUVECs.
D000001:Calcimycin / Q000494:pharmacology*; D002478:Cells, Cultured; D004727:Endothelium / Q000187:drug effects / Q000201:enzymology*; D006801:Humans; D007531:Isoflurophate / Q000031:analogs & derivatives / Q000494:pharmacology; D010960:Plasminogen Activators / Q000037:antagonists & inhibitors / Q000378:metabolism*; D015849:Plasminogen Inactivators; D013917:Thrombin / Q000031:analogs & derivatives / Q000494:pharmacology*
D015849:Plasminogen Inactivators; D007531:Isoflurophate; D000001:Calcimycin; D010960:Plasminogen Activators; C027798:DFP-alpha-thrombin; D013917:Thrombin