Studies of chemical and biologic properties of a fraction of sulodexide, a heparin-like glycosaminoglycan.
The chemical composition and biologic properties of a fraction (f) of Sulodexide, a heparin-like GAG, were studied and compared with those of two sulfated GAG preparations and heparin from intestinal mucosa. f-Sulodexide and the sulfated GAG preparations were fractionated on a Dowex-1Cl- column and subsequently on an antithrombin III affinity column. Low affinity and high affinity fractions had similar chemical composition and lipoprotein lipase releasing ability, but they varied in anticoagulant activity. Low affinity fractions from f-Sulodexide had negligible anticoagulant activity while high affinity fractions had one-half the activity of mucosal heparin. When compared to heparin, both fractions had one third amount of lipoprotein lipase releasing activity. The low anticoagulant activity of f-Sulodexide suggests a suitability for long-term use as an antiatherogenic agent.
D000818:Animals; D000925:Anticoagulants; D001777:Blood Coagulation / Q000187:drug effects; D002809:Chondroitin Sulfates / Q000302:isolation & purification; D002846:Chromatography, Affinity; D006025:Glycosaminoglycans / Q000302:isolation & purification* / Q000494:pharmacology; D006493:Heparin / Q000302:isolation & purification; D006801:Humans; D000960:Hypolipidemic Agents / Q000302:isolation & purification / Q000494:pharmacology; D066298:In Vitro Techniques; D008071:Lipoprotein Lipase / Q000378:metabolism; D011817:Rabbits
D000925:Anticoagulants; D006025:Glycosaminoglycans; D000960:Hypolipidemic Agents; C007858:glucuronyl glucosamine glycan sulfate; D006493:Heparin; D002809:Chondroitin Sulfates; D008071:Lipoprotein Lipase