Genetic variation in cytochrome P-450-dependent demethylation in Drosophila melanogaster.
The genetic variation in the basal capacity to N-demethylate aminopyrine, d-benzphetamine and ethylmorphine was studied in microsomes from adult Drosophila of 9 different strains. Ethylmorphine and d-benzphetamine N-demethylase activity varied about fourfold between the strains, with the highest capacity for both reactions in the Aflatoxin B1-sensitive Florida 9 and the lowest in the insecticide-resistant Hikone R. The two activities were closely correlated with each other but not with aminopyrine demethylation or any previously studied cytochrome P-450-dependent reaction, indicating a common determination by a separate cytochrome P-450 form(s). Aminopyrine N-demethylase activity was more than fourfold higher in the DDT-resistant Oregon R than in Berlin K. A genetic analysis of aminopyrine N-demethylation revealed that the high activity in the Oregon R(R) strain was inherited as an apparently semidominant second chromosome trait. The similar mode of inheritance as well as the close correlation between aminopyrine demethylase and the previously analysed biphenyl 4-hydroxylase activity suggests that these activities are under the same genetic control.
D000632:Aminopyrine / Q000378:metabolism; D000633:Aminopyrine N-Demethylase; D000818:Animals; D001589:Benzphetamine / Q000378:metabolism; D001711:Biotransformation; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics* / Q000378:metabolism; D004331:Drosophila melanogaster / Q000201:enzymology / Q000235:genetics*; D005036:Ethylmorphine / Q000378:metabolism; D014644:Genetic Variation; D007305:Insecticide Resistance; D008745:Methylation; D008861:Microsomes / Q000378:metabolism; D009152:Mutagenicity Tests; D010089:Oxidoreductases, N-Demethylating / Q000378:metabolism*
D000632:Aminopyrine; D001589:Benzphetamine; D003577:Cytochrome P-450 Enzyme System; C019781:benzphetamine N-demethylase; D010089:Oxidoreductases, N-Demethylating; D000633:Aminopyrine N-Demethylase; D005036:Ethylmorphine