A calibrated fluorescence polarization assay for assessment of fetal lung maturity.
The mechanism of a new fluorescence polarization method for measuring phospholipid in amniotic fluid is described. The fluorescence polarization of a surfactant fluorescent dye solubilized in amniotic fluid correlates significantly with the L/S ratio. Model studies indicate that this results primarily from partitioning of the dye between endogenous albumin, which causes a high fluorescence polarization, and dispersed phospholipid, which causes a much lower polarization. The fluorescence polarization can be compared with a calibration curve to give the ratio of phospholipid to albumin in the sample. This procedure may prove useful in antenatal assessment of fetal lung maturity.
D009327:4-Chloro-7-nitrobenzofurazan / Q000031:analogs & derivatives; D000418:Albumins / Q000032:analysis*; D000653:Amniotic Fluid / Q000032:analysis*; D005325:Fetal Organ Maturity; D005454:Fluorescence Polarization; D005456:Fluorescent Dyes; D006801:Humans; D008168:Lung / Q000196:embryology*; D008239:Lysine / Q000031:analogs & derivatives; D010713:Phosphatidylcholines / Q000032:analysis; D010743:Phospholipids / Q000032:analysis*; D013109:Sphingomyelins / Q000032:analysis
D000418:Albumins; D005456:Fluorescent Dyes; D010713:Phosphatidylcholines; D010743:Phospholipids; D013109:Sphingomyelins; C053178:PC 16; C044745:1-palmitoyl-2-(6-(N-(7-nitrobenz)-2-oxa-1,3-diazol-4-yl)aminocaproyl)phosphatidylcholine; D009327:4-Chloro-7-nitrobenzofurazan; D008239:Lysine