Accumulation of dinucleoside polyphosphates in Saccharomyces cerevisiae under stress conditions. High levels are associated with cell death.
Adenosine tetraphosphonucleosides (Ap4X) were measured in Saccharomyces cerevisiae by a coupled phosphodiesterase-luciferase assay. After exposure of the cells to cadmium or to hyperthermic treatment (46 degrees C) a marked increase of the cellular pool from 0.08 microM (base level) to 4 microM or higher was observed. The accumulation of Ap4X to high levels is associated with irreversible processes leading to cell death.
D000085:Acetates; D000227:Adenine Nucleotides / Q000378:metabolism*; D000244:Adenosine Diphosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D002104:Cadmium / Q000494:pharmacology; D015226:Dinucleoside Phosphates; D006358:Hot Temperature; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000378:metabolism*
D000085:Acetates; D000227:Adenine Nucleotides; D015226:Dinucleoside Phosphates; D002104:Cadmium; C020733:diadenosine tetraphosphate; C011440:diadenosine triphosphate; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; C028031:cadmium acetate