[Chemico-enzymatic synthesis of genetic elements for expression of synthetic genes in Bacillus subtilis cells].
In order to obtain the recombinant Bacillus subtilis strain, a transcriptional-translational control unit of the alpha-amylase gene of B. amyloliquefaciens was synthesized. The oligodeoxyribonucleotides were prepared by the modified triester method in solution and by the solid-phase approach. Then these oligonucleotides were joined by DNA ligase into two fragments which were cloned in the phage M13mp9 DNA and the plasmid pBR327. A plasmid harboring the site regulating the transcription of the alpha-amylase gene may be employed as vector for cloning the promoter-containing fragments in E. coli cells.
D001345:Autoradiography; D001412:Bacillus subtilis / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D005798:Genes, Bacterial; D005809:Genes, Regulator; D005813:Genes, Synthetic; D009693:Nucleic Acid Hybridization; D011401:Promoter Regions, Genetic; D000516:alpha-Amylases / Q000235:genetics*
D004269:DNA, Bacterial; D000516:alpha-Amylases
Khimiko-fermentativnyĭ sintez geneticheskikh élementov dlia ékspressii iskusstvennykh genov v kletkakh Bacillus subtilis.