Inhibition of ADP-ribosylation increases X-ray-induced chromosomal damage in mouse testis and bone-marrow cells in vivo.
The effect of 3-aminobenzamide (3AB) treatment on chromosomal radiosensitivity of mouse spermatogonial stem cells and bone-marrow cells was studied using various doses of X-rays. The results show that 3AB increases the induction of reciprocal translocations in slowly cycling spermatogonia as well as the frequency of chromosomal aberrations in actively dividing bone-marrow cells. The experiments indicate that both types of tissue are suitable to study the ability of inhibitors of ADP-ribosylation to modulate chromosome-breaking damage induced by ionizing radiation in vivo.
D000818:Animals; D001549:Benzamides / Q000494:pharmacology; D001853:Bone Marrow / Q000528:radiation effects*; D002869:Chromosome Aberrations; D002875:Chromosomes / Q000528:radiation effects*; D008297:Male; D051379:Mice; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D011065:Poly(ADP-ribose) Polymerases / Q000502:physiology; D013091:Spermatogenesis / Q000528:radiation effects; D013737:Testis / Q000528:radiation effects*; D014965:X-Rays
D001549:Benzamides; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; C025160:3-aminobenzamide; D011065:Poly(ADP-ribose) Polymerases