High-performance chromatographic method for the purification of tissue-type plasminogen activator.
High-performance affinity chromatography was performed on five ligand-bound columns in an attempt to purify tissue-type plasminogen activator (t-PA), which is a glycoprotein with a high affinity for fibrin and also has two Kringle structures and finger-domain in its molecule. The five columns were concanavalin A-5PW, p-aminobenzamidine-5PW, imidinodiacetic acid-5PW, boric acid-5PW and lysine-5PW. All five were able to rapidly separate t-PA from contaminating proteins, with high resolution and recovery.
D000818:Animals; D002417:Cattle; D002846:Chromatography, Affinity; D003470:Culture Media; D046508:Culture Techniques; D008545:Melanoma / Q000032:analysis; D010959:Tissue Plasminogen Activator / Q000302:isolation & purification*
D003470:Culture Media; D010959:Tissue Plasminogen Activator