Luteinizing hormone-releasing hormone (LHRH)-induced arachidonic acid release in rat granulosa cells. Role of calcium and protein kinase C.
In rat granulosa cells prelabeled with [3H]arachidonic acid, addition of LHRH, A23187 or 12-0-tetradecanoyl phorbol-13-acetate (TPA) enhanced the release of [3H]arachidonic acid into the culture medium. The effect of A23187 was significant as early as 5 min and the lowest effective dose was 5 X 10(-8)/M. On the other hand, TPA was effective only at dosages greater than 10(-6)M. These results suggest that the stimulatory effect of LHRH on arachidonic acid release is coupled more tightly to a Ca2+-dependent rather than a protein kinase C-mediated pathway.
D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism*; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000494:pharmacology*; D006107:Granulosa Cells / Q000187:drug effects* / Q000378:metabolism; D066298:In Vitro Techniques; D011493:Protein Kinase C / Q000378:metabolism; D051381:Rats; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D001095:Arachidonic Acids; D016718:Arachidonic Acid; D007987:Gonadotropin-Releasing Hormone; D000001:Calcimycin; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium