Recombinant interferon-gamma stimulates the production of human tumor necrosis factor in vitro.
Human peripheral blood monocytes in culture secrete tumor necrosis factor (TNF), which can be detected with a sensitive enzyme-linked immunosorbent assay system. When recombinant human interferon-gamma (rIFN-gamma) is added to cultured cells, TNF production is increased. rIFN-gamma is also able to sustain the elevated production level of TNF over a 4-day period. Recombinant interferon-alpha (rIFN-alpha) was unable to stimulate increased TNF production. Unstimulated mononuclear phagocytes and rIFN-alpha-treated cells decreased secretion of TNF over a 4-day interval. The stimulatory effect of rIFN-gamma was dose dependent and required both new RNA and protein synthesis and was independent of endotoxin in the tissue culture medium.
D000328:Adult; D002448:Cell Adhesion; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D006023:Glycoproteins / Q000096:biosynthesis*; D006131:Growth Inhibitors / Q000096:biosynthesis*; D006801:Humans; D007231:Infant, Newborn; D007370:Interferon Type I / Q000502:physiology; D007371:Interferon-gamma / Q000502:physiology*; D007700:Kinetics; D009000:Monocytes / Q000502:physiology; D011994:Recombinant Proteins / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha
D006023:Glycoproteins; D006131:Growth Inhibitors; D007370:Interferon Type I; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma