Basic proteins in the human aortic intima: nonequilibrium two-dimensional electrophoretic analysis of tissue extracts.
The protein composition of atheroma-free human thoracic intima was compared with that containing fatty streaks or fibro-fatty lesions utilizing two-dimensional gel electrophoresis (2-DE) and silver staining. Intimal proteins extracted with 9 M urea were separated by nonequilibrium pH gradient electrophoresis (NEPHGE) followed by polyacrylamide gel electrophoresis (PAGE) in the second dimension. NEPHGE-PAGE of proteins extracted from atheroma-free intima revealed several major proteins: actin, tropomyosin-like proteins, proteins with relative molecular weight (Mr) of 250,000 (P250), two proteins with Mr about 15,000 (P15a, P15b), and many medium proteins such as a myosin heavy chain, two myosin light chains, and proteins P47, P44, P32, P27, P20a, P20b, P19a, P19b. Several additional proteins were observed in intimas with fatty streaks and fibro-fatty lesions. Most of them, such as albumin, transferrin, Apo A-I, alpha 1-antitrypsin, fibrinogen beta-chain, IgG, appear to originate from plasma. Differences in protein composition of intima with fibro-fatty streaks compared with adjacent lesion-free intima varied from case to case and need further study. NEPHGE-PAGE in combination with isoelectric focusing (ISO)-PAGE revealed more intimal proteins in atheroma-free and diseased aortas than either method alone, proteins which might be quantitated, isolated for binding studies, and further evaluated for their potential role in atherogenesis.
D000199:Actins / Q000032:analysis; D000293:Adolescent; D000328:Adult; D000418:Albumins / Q000032:analysis; D001012:Aorta, Abdominal / Q000032:analysis*; D001013:Aorta, Thoracic / Q000032:analysis*; D016632:Apolipoprotein A-I; D001054:Apolipoproteins A / Q000032:analysis; D001161:Arteriosclerosis / Q000378:metabolism*; D002648:Child; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005340:Fibrinogen / Q000032:analysis; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007525:Isoelectric Focusing; D008297:Male; D009218:Myosins / Q000032:analysis; D011506:Proteins / Q000032:analysis*; D014168:Transferrin / Q000032:analysis; D000515:alpha 1-Antitrypsin / Q000032:analysis
D000199:Actins; D000418:Albumins; D016632:Apolipoprotein A-I; D001054:Apolipoproteins A; D007074:Immunoglobulin G; D011506:Proteins; D014168:Transferrin; D000515:alpha 1-Antitrypsin; D005340:Fibrinogen; D009218:Myosins