Effect of fibroblast interferon and recombinant gamma interferon on zymosan-induced nitroblue tetrazolium reduction in mouse peritoneal macrophages.
Mouse peritoneal macrophages (MPM) were cultivated with a fibroblast interferon (IFN) preparation or recombinant gamma-IFN (rIFN-alpha) for 1, 24 or 48 h. The zymosan-induced reduction of nitroblue tetrazolium (NBT) in these MPM was then measured. Fibroblast IFN enhanced the NBT reducing capacity of MPM when the incubation period was 1 h. When the incubation period was extended to 48 h, a suppressed NBT reduction by fibroblast IFN treated MPM was recorded. The influence of rIFN-alpha on MPM with regard to NBT reduction was minor. Only when the MPM were treated with a moderate dose of rIFN-alpha (10 U/ml) for 48 h was an enhanced NBT reduction recorded.
D000818:Animals; D005562:Formazans / Q000378:metabolism; D007370:Interferon Type I / Q000494:pharmacology*; D007371:Interferon-gamma / Q000494:pharmacology*; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D009580:Nitroblue Tetrazolium / Q000378:metabolism*; D010084:Oxidation-Reduction; D011994:Recombinant Proteins / Q000494:pharmacology; D013778:Tetrazolium Salts / Q000378:metabolism*; D015054:Zymosan / Q000494:pharmacology
D005562:Formazans; D007370:Interferon Type I; D011994:Recombinant Proteins; D013778:Tetrazolium Salts; D009580:Nitroblue Tetrazolium; D007371:Interferon-gamma; D015054:Zymosan