Effect of exogenous hormones on estrogen and progesterone release from cultured rat granulosa cells from various stages of estrous cycle.
Progesterone and estrogen levels in the culture media of granulosa cells isolated from rat ovarian follicles in all stages of estrous cycle were estimated using RIA, with special regard to hormone levels in proestrus. In control cultures the peak of progesterone release occurred at 20.00 h in proestrus. The addition of LH and FSH to the culture medium resulted in a remarkable stimulation of progesterone release by cells isolated in estrus. The highest concentration of estrogens in control cultures was observed in granulosa cells isolated at 18.00 h in proestrus. Exogenous LH and FSH stimulated the release of estrogen from the cells isolated on the days of estrus, metestrus and diestrus. Cultured granulosa cells maintained similar pattern of progesterone and estrogen release as the whole follicles did in vitro and in vivo.
D000818:Animals; D004967:Estrogens / Q000378:metabolism*; D004971:Estrus; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D006062:Gonadotropins / Q000494:pharmacology*; D006107:Granulosa Cells / Q000187:drug effects / Q000378:metabolism*; D007986:Luteinizing Hormone / Q000494:pharmacology; D011374:Progesterone / Q000378:metabolism*; D011388:Prolactin / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains
D004967:Estrogens; D006062:Gonadotropins; D011374:Progesterone; D011388:Prolactin; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone