Bacterial pneumonia stimulates macromolecule secretion and ion and water fluxes in sheep trachea.
In vivo instillation of Pasteurella haemolytica (greater than or equal to 10(7) colony-forming units/kg) into a lobar bronchus of sheep produced bacterial pneumonia by 7 days postinoculation. Infection was verified bacteriologically and histologically. Macromolecule secretion and ion and water fluxes were subsequently measured in tracheal tissues in vitro and were compared with values from sham-infected sheep. Macromolecules were radiolabeled with 35SO4 and [3H]threonine, and we measured the secretion of macromolecule-bound radiolabel onto the mucosa. Unidirectional fluxes of Cl-, Na+, and water were measured with radioactive tracers under open-circuit and short-circuit conditions. Lung infection increased basal secretion of bound 35SO4 (by 189%) and bound [3H]-threonine (by 110%). It significantly increased net Na+ absorption under open- and short-circuit conditions and induced open-circuit net absorption of Cl- and water (16 +/- 29 microliters X cm-2 X h-1). These changes were associated with specific recruitment of neutrophils and elevated levels of arachidonate metabolites (thromboxane B2 and leukotriene B4) in the airways. Thus the bacterial pneumonia-induced changes in tracheal mucus secretion may be the result of airway inflammation.
D000818:Animals; D001834:Body Water / Q000378:metabolism*; D005260:Female; D007477:Ions / Q000378:metabolism*; D008433:Mathematics; D010326:Pasteurella Infections / Q000378:metabolism*; D010539:Permeability; D011014:Pneumonia / Q000378:metabolism* / Q000382:microbiology; D012756:Sheep; D013431:Sulfates / Q000378:metabolism; D013912:Threonine / Q000378:metabolism; D013929:Thromboxane B2 / Q000378:metabolism; D014132:Trachea / Q000378:metabolism*
D007477:Ions; D013431:Sulfates; D013912:Threonine; D013929:Thromboxane B2