title: Profiling of endogenous ligand solutes that bind to serum proteins in sera of patients with uremia.
abstract: The profiling of endogenous ligand solutes in sera of patients with uremia was performed by using an ultrafiltration device and high-performance liquid chromatography. Seventeen endogenous ligand solutes, which are ultraviolet-absorbing substances, were detected in a sample volume of 40 microliters, and four ligand solutes were tentatively identified as indoxylsulfate, hippuric acid, 2-hydroxybenzoylglycine, and 3-indoleacetic acid. One of these ligand solutes designated as peak P was thought to be a candidate for a major drug-binding inhibitor in uremia.
mesh_terms: D001798:Blood Proteins / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D006626:Hippurates / Q000097:blood; D006358:Hot Temperature; D006801:Humans; D007200:Indican / Q000097:blood; D007210:Indoleacetic Acids / Q000097:blood; D008024:Ligands; D011485:Protein Binding; D014462:Ultrafiltration; D014511:Uremia / Q000097:blood*
chemical_list: D001798:Blood Proteins; D006626:Hippurates; D007210:Indoleacetic Acids; D008024:Ligands; C000693:salicylurate; C030737:indoleacetic acid; D007200:Indican; C030514:hippuric acid