A p60v-src-related tyrosine kinase in the acetylcholine receptor-rich membranes of Narke japonica: association and dissociation of phosphatidylinositol kinase activity.
We have isolated a tyrosine-specific protein kinase from the acetylcholine receptor (AChR)-rich membranes of the electric ray Narke japonica. The enzyme is immunologically related to p60v-src, the product of the transforming gene of Rous sarcoma virus. A substantial phosphatidylinositol (PI) kinase activity was associated with this enzyme when it was purified through tyrosine-agarose affinity chromatography used previously for the purification of p60v-src. However, by subsequent chromatography on casein-agarose, most of the associated PI kinase activity was separated from the tyrosine kinase activity. The results suggest that the tyrosine-specific protein kinase in the AChR-rich membranes of N. japonica has no intrinsic PI kinase activity.
D000818:Animals; D002846:Chromatography, Affinity; D015688:Oncogene Protein pp60(v-src); D010716:Phosphatidylinositols / Q000378:metabolism*; D011494:Protein Kinases / Q000378:metabolism*; D011505:Protein-Tyrosine Kinases / Q000302:isolation & purification*; D011950:Receptors, Cholinergic / Q000032:analysis*; D012191:Retroviridae Proteins / Q000378:metabolism*; D014101:Torpedo
D010716:Phosphatidylinositols; D011950:Receptors, Cholinergic; D012191:Retroviridae Proteins; D011494:Protein Kinases; D011505:Protein-Tyrosine Kinases; D015688:Oncogene Protein pp60(v-src)