[Ultrastructural detection of messenger RNA coding for growth hormone in the rat anterior pituitary by in situ hybridization].
Ultrastructural detection of the messenger RNA coding for growth hormone in rat pituitary gland could be obtained by association of in situ hybridization and cryoultramicrotomy. Messenger RNAs were localized in the anterior pituitary gland. Silver grain densities observed in autoradiograms after in situ hybridization were dependent to incubation period and to fixation. It was necessary to determine a compromise between ultrastructural aspect and silver grain densities. Messenger RNAs were detected in somatotropic cells, identified by ultrastructural characteristics, in both the nucleus (euchromatin and nuclear membrane) and cytoplasm, in vicinity to endoplasmic reticulum.
D000818:Animals; D001345:Autoradiography; D013006:Growth Hormone / Q000235:genetics*; D008297:Male; D008854:Microscopy, Electron; D009693:Nucleic Acid Hybridization; D010903:Pituitary Gland, Anterior / Q000378:metabolism* / Q000648:ultrastructure; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics*; D051381:Rats; D012834:Silver; D013462:Sulfur Radioisotopes; D014316:Tritium
D012333:RNA, Messenger; D013462:Sulfur Radioisotopes; D014316:Tritium; D012834:Silver; D013006:Growth Hormone
Détection ultrastructurale des ARN messagers codant pour l'hormone de croissance dans l'antéhypophyse du rat par hybridation in situ.