A heterogeneous beta-D-glucosidase activator from monkey parotid gland and its use as an affinity ligand in the purification of human salivary beta-D-glucosidase.
We have isolated a heat-stable, low molecular weight activator peptide(s) from monkey parotid gland that specifically activated human salivary beta-D-glucosidase. This activator appeared to be heterogeneous on Sephadex G-25 gel filtration and polyacrylamide gel electrophoresis under non-denaturing conditions. About 45% of the human salivary beta-glucosidase could bind to the activator immobilised on Sepharose and be eluted by Cutscum. The purified enzyme was nearly homogeneous, with a subunit Mr of 46,000 as revealed by SDS-gel electrophoresis and silver staining.
D000818:Animals; D002846:Chromatography, Affinity / Q000379:methods*; D002850:Chromatography, Gel; D004789:Enzyme Activation; D005959:Glucosidases / Q000302:isolation & purification*; D006023:Glycoproteins; D006801:Humans; D008255:Macaca radiata; D010306:Parotid Gland / Q000032:analysis*; D011506:Proteins / Q000302:isolation & purification* / Q000378:metabolism; D012463:Saliva / Q000201:enzymology*; D012471:Salivary Proteins and Peptides / Q000302:isolation & purification*; D049231:Saposins; D001617:beta-Glucosidase / Q000302:isolation & purification*
D006023:Glycoproteins; D011506:Proteins; D012471:Salivary Proteins and Peptides; D049231:Saposins; D005959:Glucosidases; D001617:beta-Glucosidase