Isolation and characterization of a lipopolysaccharide-specific bacteriophage of Pseudomonas aeruginosa.
Phage H22 was isolated from sewage using Pseudomonas aeruginosa NCTC 8505 (serotype 0:3) as the host. Although not O-specific, this phage was found to have lipopolysaccharide (LPS) as a receptor. The broad host-range and lack of O-specificity of the phage suggested that its receptor site was in the core region of the LPS. Phage H22 had a Bradley type A structure. It was unaffected by chloroform and diethyl ether, and was stable between pH 5 and 8 and in the temperature range 0 to 60 degrees C. The adsorption rate constant was 14.6 X 10(-9) ml min-1. The phage had a latent period of 43 min, with a rise time of 18 min and a burst size of 6. The adsorption of phage to whole cells and LPS occurred over a broad pH range. Maximum adsorption occurred at 50 degrees C and pH 7.5 in the presence of 0.001 M Ca2+.
D000327:Adsorption; D001435:Bacteriophages / Q000145:classification* / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism / Q000648:ultrastructure; D006863:Hydrogen-Ion Concentration; D008070:Lipopolysaccharides / Q000378:metabolism*; D008854:Microscopy, Electron; D011550:Pseudomonas aeruginosa; D011991:Receptors, Virus / Q000378:metabolism*; D012722:Sewage; D013045:Species Specificity; D013696:Temperature
D008070:Lipopolysaccharides; D011991:Receptors, Virus; D012722:Sewage