Quantitation and estimation of lymphocyte subsets in tissue sections. Comparison with flow cytometry.
A quantitation method for lymphocyte subsets in immunoperoxidase-stained frozen tissue sections was compared with flow cytometry in 23 cases of non-Hodgkin's lymphoma. Close correlations were obtained, demonstrating the accuracy of the technic. Weak intensity of fluorescence and fragility of the tumor cells during the fluorescence-activated cell sorter (FACS) analyses were the most likely explanations for a number of the discrepancies observed. The tissue quantitation method was precise, particularly at low values, where it was better than the FACS. A simpler and faster estimation method employing categories within 10 percentage units was also tested in this study; this method correlated as well with the FACS as the quantitation method and gave the best interobserver correlations.
D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface / Q000032:analysis; D001402:B-Lymphocytes / Q000145:classification; D002452:Cell Count / Q000379:methods*; D002469:Cell Separation; D005434:Flow Cytometry; D005629:Frozen Sections; D006801:Humans; D007124:Immunoenzyme Techniques; D008214:Lymphocytes / Q000166:cytology* / Q000276:immunology; D008228:Lymphoma, Non-Hodgkin / Q000473:pathology; D012189:Retrospective Studies; D013601:T-Lymphocytes / Q000145:classification
D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface