Application of an enzyme-linked immunosorbent assay in the final stages of a bovine brucellosis eradication program.
Bovine field serums from the Australian brucellosis eradication program were used to compare 2 enzyme linked immunosorbent assays (ELISA) with the complement fixation test (CFT) and Rose Bengal test (RBT). One ELISA used an anti-bovine IgG horseradish peroxidase conjugate (ELISA 1) and the other a monoclonal anti-bovine Ig alkaline phosphatase conjugate (ELISA 2). When compared with the CFT, the ELISA 2 like the ELISA 1 lacked specificity in B. abortus vaccinated herds but the ELISA 2 was more specific than the ELISA 1 in previously infected herds and equally as specific as the ELISA 1 in nonvaccinated Brucella free herds. In this study the ELISA 2 proved more sensitive than the CFT, RBT and ELISA 1 particularly in herds where B. abortus biotype 2 was present. The value of using the ELISA 2 in conjunction with the CFT in an eradication program is discussed.
D000372:Agglutination Tests; D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis*; D002004:Brucella Vaccine; D002003:Brucella abortus / Q000276:immunology*; D002007:Brucellosis, Bovine / Q000175:diagnosis* / Q000276:immunology / Q000517:prevention & control; D002417:Cattle; D003168:Complement Fixation Tests; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D011247:Pregnancy; D012395:Rose Bengal; D014611:Vaccination / Q000662:veterinary
D000907:Antibodies, Bacterial; D002004:Brucella Vaccine; D012395:Rose Bengal