Phage resistance in Streptococcus lactis ssp. diacetylactis transconjugant SLA3.2501 and its derivatives.
Phage 18-16, which was virulent for Streptococcus lactis ssp. diacetylactis SLA3.25 was used to study phage-resistant characteristics of mucoid S. lactis ssp. diacetylactis transconjugant SLA3.2501 obtained through conjugative cotransfer of pSRQ2201 (Lac-plasmid) and pSRQ2202 (Muc-plasmid) to SLA3.25 (15). Interaction of phage 18-16 with SLA3.2501 and its derivatives showed that phage resistance was not related to either the lack of phage adsorption or restriction-modification. Suppression of phage replication in SLA3.2501 and its derivatives was not completely relieved by curing of either pSRQ2201 or pSRQ2202 or both.
D001435:Bacteriophages / Q000502:physiology*; D003227:Conjugation, Genetic; D013294:Lactococcus lactis / Q000235:genetics / Q000502:physiology*; D010957:Plasmids