Separation and purification of individual neurofilament proteins by reverse-phase high-performance liquid chromatography.
A reverse-phase HPLC method was developed to separate individual neurofilament proteins (210,000, 160,000 and 70,000 Da) from the glial fibrillary acid protein. It is useful for analytical or preparative methods, with yields higher than 80%. The method represents improvement over previous methods in speed, efficiency, and purity. Combining this HPLC method with the conventional chromatographic method on DEAE-cellulose, highly purified individual neurofilament proteins can be obtained in large scale.
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005904:Glial Fibrillary Acidic Protein / Q000302:isolation & purification; D007381:Intermediate Filament Proteins / Q000302:isolation & purification*; D008970:Molecular Weight; D016900:Neurofilament Proteins; D051381:Rats
D005904:Glial Fibrillary Acidic Protein; D007381:Intermediate Filament Proteins; D016900:Neurofilament Proteins; C044882:neurofilament protein L