[Development of sensitivity to glycine, GABA and beta-alanine in cultured spinal neurons].
Whole cell patch-clamp recording of cultured chick spinal neurons, presumed to be motoneurons, revealed currents elicited in these cells by GABA, glycine and beta-alanine, corresponding to the opening of chloride channels. During maturation, sensitivity to all three transmitters were first detected on the day 6 of culture, and appeared in most neurons by day 8. However, at all stages of development, a fraction of the cells were sensitive only to GABA; this observation supports the notion that the GABA and the glycine receptors are distinct. On the other hand, separate activation by glycine and beta-alanine was never observed, in agreement with the postulate that these amino acids bind to the same receptor.
D000409:Alanine / Q000494:pharmacology*; D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D005998:Glycine / Q000494:pharmacology*; D008564:Membrane Potentials / Q000187:drug effects; D009474:Neurons / Q000187:drug effects*; D013116:Spinal Cord / Q000166:cytology / Q000187:drug effects*; D013997:Time Factors; D015091:beta-Alanine / Q000494:pharmacology*; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology*
D015091:beta-Alanine; D005680:gamma-Aminobutyric Acid; D000409:Alanine; D005998:Glycine
Evolution des sensibilités à la glycine, au GABA et à la beta-alanine de neurones spinaux en culture.