[Development of a latex agglutination method for the diagnosis of Pseudomonas aeruginosa infection].
The present investigation has revealed the possibility of using different kinds of monodispersed polystyrene latex, produced in the USSR, as carriers in the process of the preparation of antibody diagnosticums intended for the detection of water-soluble slime antigens of Pseudomonas aeruginosa strains belonging to the most widespread serological types. The optimum conditions for the preparation of latex reagents and for making the latex-agglutination test have been experimentally established. The new diagnosticums+ have been shown to be highly species- and type-specific, which permits making judgment on the presence or absence of slime antigens of P. aeruginosa strains belonging to definite serovars in the clinical material under study. The preparations thus obtained have been found to retain their sensitivity for 16 months (the term of observation).
D000907:Antibodies, Bacterial / Q000276:immunology; D000942:Antigens, Bacterial / Q000032:analysis; D005069:Evaluation Studies as Topic; D006801:Humans; D007202:Indicators and Reagents; D007840:Latex; D007841:Latex Fixation Tests / Q000379:methods*; D010316:Particle Size; D011552:Pseudomonas Infections / Q000175:diagnosis*; D011550:Pseudomonas aeruginosa / Q000276:immunology; D012995:Solubility; D013045:Species Specificity
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D007202:Indicators and Reagents; D007840:Latex
Razrabotka metoda lateks-aggliutinatsii dlia diagnostiki sinegnoĭnoĭ infektsii.