title: Peptides with antilipolytic and lipogenic activities from seeds of the bitter gourd Momordica charantia (family Cucurbitaceae).
abstract: Decorticated Momordica charantia seeds were extracted with acidic ethanol. The pH of the extract was adjusted to 8 and the resulting precipitate was chromatographed on CM Sepharose CL-6B and then desalted on Sephadex G-10. A number of factors which exhibited antilipolytic and lipogenic activities in rat adipocytes were isolated. Two of the factors were demonstrated to be peptides with similar amino acid compositions and a molecular weight of approximately 8000. However the peptides greatly differed in their chromatographic behavior on CM-Sepharose CL-6B and in their mobilities in agarose and polyacrylamide gel electrophoresis.
mesh_terms: D000020:Abortifacient Agents, Nonsteroidal / Q000302:isolation & purification; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002850:Chromatography, Gel; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006386:Hemagglutination Tests; D006461:Hemolysis / Q000187:drug effects; D000960:Hypolipidemic Agents / Q000302:isolation & purification*; D005779:Immunodiffusion; D008055:Lipids / Q000096:biosynthesis*; D051379:Mice; D008813:Mice, Inbred ICR; D008970:Molecular Weight; D009699:N-Glycosyl Hydrolases; D010455:Peptides / Q000302:isolation & purification / Q000494:pharmacology*; D010940:Plant Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D054790:Ribosome Inactivating Proteins, Type 2; D012639:Seeds / Q000032:analysis; D012967:Sodium Dodecyl Sulfate
chemical_list: D000020:Abortifacient Agents, Nonsteroidal; D000596:Amino Acids; D000960:Hypolipidemic Agents; D008055:Lipids; D010455:Peptides; D010940:Plant Proteins; D054790:Ribosome Inactivating Proteins, Type 2; D012967:Sodium Dodecyl Sulfate; D009699:N-Glycosyl Hydrolases