Rat liver UDP-glucuronosyltransferase. Identification of cDNAs encoding two enzymes which glucuronidate testosterone, dihydrotestosterone, and beta-estradiol.
The 1818-base pairs cDNA encoding a form of rat liver UDP-glucuronosyltransferase designated UDP-GTr-3 was sequenced and found to encode a protein of 530 amino acids (Mr = 60,522). Characteristic sequences include a signal peptide and a carboxyl-terminal transmembrane anchoring region. There were no potential asparagine-linked glycosylation sites. Transcription and translation of the cDNA in vitro showed that the encoded protein was synthesized as a precursor and was cleaved when dog pancreatic microsomes were present during translation. Cleaved UDPGTr-3 was intrinsically associated with the added membranes, whereas uncleaved polypeptide remained in the supernatant upon fractionation of the translation mixture. UDPGTr-3 and a related phenobarbital-inducible form of UDP-glucuronosyltransferase (designated UDPGTr-2) were both expressed in COS cells and their capacities to glucuronidate 13 commonly used substrates were analyzed. Whereas both enzymes glucuronidated the endogenous steroids testosterone, dihydrotestosterone, and beta-estradiol, only UDPGTr-2 was active towards the foreign chemical substrates, chloramphenicol, 4-hydroxybiphenyl, and 4-methylumbelliferone. Neither enzyme was active towards estrone, androsterone and substrates typical of 3-methylcholanthrene-inducible forms of UDP-glucuronosyltransferase. Steady-state levels of UDPGTr-3 and UDPGTr-2 mRNAs were highest in the liver and were barely detectable in kidney, lung, testis, and small intestinal mucosa. These data show that at least two forms of UDP-glucuronosyltransferase found predominantly in the liver have evolved to glucuronidate the same endogenous steroid substrates and that the phenobarbital-inducible form also has some activity towards foreign compounds.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism*; D013196:Dihydrotestosterone / Q000378:metabolism; D004958:Estradiol / Q000378:metabolism; D005796:Genes; D014453:Glucuronosyltransferase / Q000235:genetics* / Q000378:metabolism; D008099:Liver / Q000201:enzymology*; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology; D014176:Protein Biosynthesis; D051381:Rats; D011919:Rats, Inbred Strains; D013739:Testosterone / Q000378:metabolism; D014158:Transcription, Genetic
D013196:Dihydrotestosterone; D013739:Testosterone; D004958:Estradiol; D004247:DNA; D014453:Glucuronosyltransferase