The use of insulin-enriched culture medium and human-human hybridoma formation: a preliminary study.
Human lymphoblastoid cell line WI-L2-729-HFZ was fused with human lymph-node lymphocytes in one fusion and with human spleen cells in another fusion to generate human-human hybridomas. In both, increasing doses of insulin were added to the HAT medium immediately after the PEG-mediated cell fusion (10(-1)-10(-5) IU/ml) and the number of clones formed was determined 3 weeks later. 10(-3) IU/ml of insulin resulted in a 2- to 5-fold increase in the number of clones generated compared to the control plates. In view of the known difficulties in generating high-yield human-human hybridoma fusions, it is suggested that the use of insulin-supplemented HAT medium may provide a more efficient way in obtaining such clones.
D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D002459:Cell Fusion; D003470:Culture Media / Q000494:pharmacology*; D006147:Guanine / Q000494:pharmacology; D006801:Humans; D006825:Hybridomas / Q000166:cytology*; D019271:Hypoxanthine; D007042:Hypoxanthines / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology*; D008214:Lymphocytes / Q000166:cytology; D013936:Thymidine / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D003470:Culture Media; D007042:Hypoxanthines; D007328:Insulin; D019271:Hypoxanthine; D006147:Guanine; D013936:Thymidine