Thermal gels: a procedure for determination of heat-inactivation temperature of enzymes.
A facile procedure for the study of inactivation temperatures of enzymes that could be stained for activity after electrophoresis is described. This is particularly suitable for enzymes that have multiple electrophoretic forms. The results of studies on Drosophila dehydrogenases and acetylcholinesterase are presented. The procedure may be useful in the study of genetic variants.
D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D004331:Drosophila melanogaster / Q000201:enzymology; D004591:Electrophoresis, Polyacrylamide Gel; D004798:Enzymes / Q000235:genetics / Q000378:metabolism*; D014644:Genetic Variation; D005969:Glutamate Dehydrogenase / Q000037:antagonists & inhibitors / Q000378:metabolism; D005993:Glycerolphosphate Dehydrogenase / Q000037:antagonists & inhibitors / Q000378:metabolism; D006358:Hot Temperature; D013053:Spectrophotometry
D004798:Enzymes; D005993:Glycerolphosphate Dehydrogenase; D005969:Glutamate Dehydrogenase; D000110:Acetylcholinesterase