The cloning and characterization of a RAS gene from Schizosaccharomyces pombe.
We have cloned and determined the complete nucleotide sequence of a RAS gene from the yeast Schizosaccharomyces pombe (SP-RAS). The putative RAS protein of 214 amino acids is encoded by two noncontiguous reading frames separated by an intron of 86 bp. The SP-RAS gene product shares extensive homology with the proteins of the Saccharomyces cerevisiae (SC), Dictyostelium, Drosophila, and human RAS genes in its N-terminal region but not in its C-terminal region. The extended C-terminal regions found in the SC-RAS genes have no counterpart in the SP-RAS gene. Thus the RAS genes of these two yeasts are structurally quite distinct. The SP-RAS sequence was expressed in vivo.
D001483:Base Sequence; D003001:Cloning, Molecular; D004274:DNA, Recombinant / Q000032:analysis; D004023:Dictyostelium / Q000235:genetics; D004330:Drosophila / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics*; D005800:Genes, Fungal; D006801:Humans; D009363:Neoplasm Proteins / Q000235:genetics; D015689:Oncogene Protein p21(ras); D009857:Oncogenes; D011518:Proto-Oncogene Proteins / Q000235:genetics; D016283:Proto-Oncogene Proteins p21(ras); D012441:Saccharomyces cerevisiae / Q000235:genetics; D004718:Saccharomycetales / Q000235:genetics*; D012568:Schizosaccharomyces / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D018631:ras Proteins
D004274:DNA, Recombinant; D005656:Fungal Proteins; D009363:Neoplasm Proteins; D011518:Proto-Oncogene Proteins; C489016:ras protein, Dictyostelium; C501009:HRAS protein, human; D015689:Oncogene Protein p21(ras); D016283:Proto-Oncogene Proteins p21(ras); D018631:ras Proteins