Sequence and evolution of HLA-DR7- and -DRw53-associated beta-chain genes.
cDNA clones representing products of the DR7 and DRw53 beta-chain genes were isolated from the human B-lymphoblastoid cell line MANN (DR7,DRw53,DQw2, DPw2). The DRw53 beta sequence was identical to a DRw53 beta sequence derived from cells with a DR4 haplotype. In contrast, the DR7 beta sequence was as unrelated to DR4 beta sequence as it was to other DR beta-related genes, except at the 3'-untranslated region. These results suggest that the DR7 and DR4 haplotypes may have been derived relatively recently from a common ancestral haplotype and that the DR4 and DR7 beta-chain genes have undergone more rapid diversification in their beta 1 domains, most probably as a result of natural selection, than have the DRw53 beta-chain genes. Short tracts of sequence within the DR7 and DRw53 beta 1 domains were shared with other DR beta sequences, indicating that exchanges of genetic information between beta 1 domains of DR beta-related genes have played a part in their evolution. Serological analysis of mouse L-cell transfectants expressing surface HLA-DR7 molecules, confirmed by antibody binding and allelic sequence comparisons, identified amino acid residues that may be critical to the binding of a monomorphic DR- and DP-specific monoclonal antibody.
D000595:Amino Acid Sequence; D000818:Animals; D001402:B-Lymphocytes / Q000032:analysis; D001483:Base Sequence; D002460:Cell Line; D004247:DNA / Q000235:genetics; D005785:Gene Conversion; D005796:Genes; D005802:Genes, MHC Class II; D006681:HLA-D Antigens / Q000235:genetics*; D006684:HLA-DR Antigens / Q000235:genetics*; D015809:HLA-DR7 Antigen; D059845:HLA-DRB4 Chains; D006801:Humans; D007739:L Cells; D051379:Mice; D011110:Polymorphism, Genetic
D006681:HLA-D Antigens; D006684:HLA-DR Antigens; C050610:HLA-DR53; D015809:HLA-DR7 Antigen; D059845:HLA-DRB4 Chains; D004247:DNA