Modulation of class II (DR and DQ) antigen expression on gingival Langerhans cells in vitro by gamma interferon and prostaglandin E2.
The effect of human and murine gamma interferon (IFN-gamma) on Class II (DR and DQ) expression by human gingival Langerhans cells (LC) was examined using an organ culture system. Human and murine IFN-gamma increased the expression of both DR and DQ antigens during a 72-h period. Maximal levels of DR expression (100% of T6+ cells) were produced within 6 h of IFN-gamma treatment and were maintained during culture. DQ expression was maximal (100% of T6+ cells) at 18 h and was more labile than DR. The effect of IFN-gamma on DR and DQ was abrogated by prostaglandin E2 (PGE) while indomethacin, and inhibitor of PGE synthesis, mimicked the effect of IFN-gamma on DR and DQ expression. Gingival keratinocytes maintained their expression of DR for 72 h in the presence of gamma-IFN, but did not express DQ under any of the culture conditions used in this study. These results demonstrate that gamma-IFN induces differential expression of Class II antigens in the oral mucosa in vitro, and suggest that gamma-IFN and PGE may be involved in the control of DR and DQ antigen expression in vivo.
D000818:Animals; D015232:Dinoprostone; D004305:Dose-Response Relationship, Drug; D005881:Gingiva / Q000166:cytology*; D006681:HLA-D Antigens / Q000032:analysis*; D006683:HLA-DQ Antigens / Q000032:analysis*; D006684:HLA-DR Antigens / Q000032:analysis*; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology; D007371:Interferon-gamma / Q000008:administration & dosage / Q000494:pharmacology*; D007801:Langerhans Cells / Q000187:drug effects / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009924:Organ Culture Techniques; D011458:Prostaglandins E / Q000008:administration & dosage / Q000494:pharmacology*
D006681:HLA-D Antigens; D006683:HLA-DQ Antigens; D006684:HLA-DR Antigens; D011458:Prostaglandins E; D007371:Interferon-gamma; D015232:Dinoprostone; D007213:Indomethacin