title: A detailed study of gerJ mutants of Bacillus subtilis.
abstract: A total of nine gerJ mutants have now been isolated in Bacillus subtilis. All are defective in their spore germination properties, being blocked at an intermediate (phase grey) stage. The dormant spores are sensitive to heating at 90 degrees C and two of the mutants (generated by transposon insertion) produce spores sensitive at 80 degrees C. The spores of these two more extreme mutants had a visibly defective cortex when studied by electron microscopy, as did some of the other mutants. During sporulation, the acquisition of spore resistance properties and the appearance of the sporulation-specific penicillin-binding protein PBP5* were delayed. A strain probably carrying a lacZ fusion to the gerJ promoter demonstrated increased expression between t2 and t4. We propose that the gerJ locus is involved in the control of one or more sporulation-specific genes.
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics* / Q000502:physiology; D001426:Bacterial Proteins; D002352:Carrier Proteins / Q000096:biosynthesis; D003114:Colony-Forming Units Assay; D006602:Hexosyltransferases; D006358:Hot Temperature; D008854:Microscopy, Electron; D002267:Muramoylpentapeptide Carboxypeptidase / Q000096:biosynthesis; D009154:Mutation; D046915:Penicillin-Binding Proteins; D010458:Peptidyl Transferases; D013171:Spores, Bacterial / Q000648:ultrastructure; D014170:Transformation, Genetic
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D046915:Penicillin-Binding Proteins; D010458:Peptidyl Transferases; D006602:Hexosyltransferases; D002267:Muramoylpentapeptide Carboxypeptidase