title: Stimulation of IgE sensitized human alveolar macrophages by anti-IgE is unaffected by sodium cromoglycate.
abstract: Sodium cromoglycate is an effective prophylactic in the treatment of asthma. However, its mechanism of action is still uncertain. The release of thromboxane B2 and N-acetyl-beta-D-glucosaminidase activity was measured from human alveolar macrophages sensitized with human myeloma IgE. Cells were challenged with rabbit affinity purified anti-IgE (no activity against IgG) in the absence or presence of sodium cromoglycate (10(-4)-10(-8)M). There was no change in the release of either thromboxane B2 or N-acetyl-beta-D-glucosaminidase in the presence of sodium cromoglycate at these concentrations.
mesh_terms: D000118:Acetylglucosaminidase / Q000378:metabolism; D000888:Antibodies, Anti-Idiotypic / Q000494:pharmacology*; D000936:Antigen-Antibody Complex / Q000276:immunology; D002478:Cells, Cultured; D004205:Cromolyn Sodium / Q000494:pharmacology*; D006801:Humans; D007114:Immunization; D007073:Immunoglobulin E / Q000276:immunology* / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects* / Q000201:enzymology / Q000276:immunology / Q000378:metabolism; D011650:Pulmonary Alveoli / Q000166:cytology*; D013268:Stimulation, Chemical; D007507:Therapeutic Irrigation; D013929:Thromboxane B2 / Q000378:metabolism
chemical_list: D000888:Antibodies, Anti-Idiotypic; D000936:Antigen-Antibody Complex; C026408:anti-IgE antibodies; D007073:Immunoglobulin E; D013929:Thromboxane B2; D000118:Acetylglucosaminidase; D004205:Cromolyn Sodium