Identification of epsilon-N-mono-, epsilon-N-di-, and epsilon-N-trimethyllysine by high-performance liquid chromatography.
Dansyl derivatives of epsilon-N-mono-, epsilon-N-di-, and epsilon-N-trimethyllysine were resolved from other amino acids in proteins by the use of high-performance liquid chromatography. The system was tested with amino acid standard combinations as well as with acid-hydrolyzed proteins known to contain methylated residues. In all cases the methylated lysines were well resolved.
D002851:Chromatography, High Pressure Liquid; D008239:Lysine / Q000031:analogs & derivatives* / Q000032:analysis
C005205:epsilon-N-methyllysine; C018926:epsilon N-dimethyllysine; C003712:trimethyllysine; D008239:Lysine