beta-Lactamase production in experimental endocarditis due to aminoglycoside-resistant Streptococcus faecalis.
We used a beta-lactamase-producing (beta L+) strain of Streptococcus faecalis that also had high levels of resistance to all aminoglycosides to induce experimental endocarditis in rats. The rats were treated for five or 10 days with procaine penicillin, vancomycin, gentamicin, rifampin, or ciprofloxacin (alone or in various combinations), or with penicillin plus clavulanic acid. The levels of penicillin in serum and vegetations declined rapidly in the beta L+-infected rats treated with procaine penicillin alone, unlike the sustained levels of penicillin in either beta L- -infected rats treated with procaine penicillin or beta L+-infected rats treated with penicillin plus clavulanic acid. For the beta L+-infected rats, the enterococcal counts in vegetations were significantly reduced (greater than 3 log10 cfu/g) only by vancomycin and by penicillin plus clavulanic acid. The efficacy of the latter regimen probably resulted from the inhibition of penicillin inactivation by clavulanic acid in vegetations infected with the beta L+ strain. Our in vivo findings document the biologic significance of beta-lactamase production.
D000617:Aminoglycosides / Q000032:analysis / Q000494:pharmacology / Q000627:therapeutic use; D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis / Q000494:pharmacology* / Q000627:therapeutic use; D019818:Clavulanic Acid; D002969:Clavulanic Acids / Q000627:therapeutic use; D004697:Endocarditis, Bacterial / Q000188:drug therapy / Q000382:microbiology*; D013293:Enterococcus faecalis / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D010402:Penicillin G Procaine / Q000032:analysis / Q000494:pharmacology / Q000627:therapeutic use; D010403:Penicillin Resistance; D051381:Rats; D011919:Rats, Inbred Strains; D013290:Streptococcal Infections / Q000188:drug therapy / Q000382:microbiology*; D014640:Vancomycin / Q000032:analysis / Q000494:pharmacology / Q000627:therapeutic use; D001618:beta-Lactamases / Q000096:biosynthesis*
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D002969:Clavulanic Acids; D010402:Penicillin G Procaine; D019818:Clavulanic Acid; D014640:Vancomycin; D001618:beta-Lactamases