A histological comparison of phase-partition fixation with fixation in aqueous solutions.
In phase-partition fixation, tissue is immersed in a non-aqueous solvent at equilibrium with an aqueous solution of a fixing agent to minimize osmotic effects. Preservation of morphology afforded by phase-partition fixation using formalin and glutaraldehyde and several organic solvents was compared to aqueous 10% neutral buffered formalin fixation for five tissues. It was shown that phase-partition fixation can provide excellent fixation for light microscopy if the proper combinations of fixatives and solvents are used.
D000818:Animals; D005404:Fixatives; D005557:Formaldehyde; D005976:Glutaral; D006321:Heart / Q000033:anatomy & histology*; D007421:Intestine, Small / Q000032:analysis*; D007668:Kidney / Q000033:anatomy & histology*; D008099:Liver / Q000033:anatomy & histology*; D010179:Pancreas / Q000033:anatomy & histology*; D051381:Rats; D011919:Rats, Inbred Strains; D012996:Solutions; D012997:Solvents; D014867:Water
D005404:Fixatives; D012996:Solutions; D012997:Solvents; D014867:Water; D005557:Formaldehyde; D005976:Glutaral