Identification of O-methylsterigmatocystin as an aflatoxin B1 and G1 precursor in Aspergillus parasiticus.
An isolate of Aspergillus parasiticus CP461 (SRRC 2043) produced no detectable aflatoxins, but accumulated O-methylsterigmatocystin (OMST). When sterigmatocystin (ST) was fed to this isolate in a low-sugar medium, there was an increase in the accumulation of OMST, without aflatoxin synthesis. When radiolabeled [14C]OMST was fed to resting mycelia of a non-aflatoxin-, non-ST-, and non-OMST-producing mutant of A. parasiticus AVN-1 (SRRC 163), 14C-labeled aflatoxins B1 and G1 were produced; 10 nmol of OMST produced 7.8 nmol of B1 and 1.0 nmol of G1, while 10 nmol of ST produced 6.4 nmol of B1 and 0.6 nmol of G1. A time course study of aflatoxin synthesis in ST feeding experiments with AVN-1 revealed that OMST is synthesized by the mold during the onset of aflatoxin synthesis. The total amount of aflatoxins recovered from OMST feeding experiments was higher than from experiments in which ST was fed to the resting mycelia. These results suggest that OMST is a true metabolite in the aflatoxin biosynthetic pathway between sterigmatocystin and aflatoxins B1 and G1 and is not a shunt metabolite, as thought previously.
D016604:Aflatoxin B1; D000348:Aflatoxins / Q000096:biosynthesis*; D001230:Aspergillus / Q000378:metabolism*; D013241:Sterigmatocystin / Q000031:analogs & derivatives / Q000096:biosynthesis* / Q000378:metabolism*; D014966:Xanthenes / Q000096:biosynthesis* / Q000378:metabolism*
D000348:Aflatoxins; D014966:Xanthenes; D013241:Sterigmatocystin; C039904:O-methylsterigmatocystin; C027955:aflatoxin G1; D016604:Aflatoxin B1