[Improved method of lipopolysaccharide isolation from gram-negative bacteria].
A modification of the traditional method for lipoplysaccharide isolation from the cells of grammnegative bacteria was elaborated on the basis of extraction by the hot water solution of phenol (the method of Westfahl). To make the method simpler and to raise the yield of the product it was proposed to use the water-phenol extract without its division for plases. The nucleic acids are eliminated by precipitation from dialyzed extract at pH 3,2-3,4 achieved by addition of acetic acid. The comparative isolation of lipopolysaccharides by the classic and modified methods has confirmed the advantages of a new technique.
D006090:Gram-Negative Bacteria / Q000032:analysis* / Q000276:immunology; D008070:Lipopolysaccharides / Q000302:isolation & purification*; D008722:Methods; D011550:Pseudomonas aeruginosa / Q000032:analysis / Q000276:immunology; D012761:Shigella boydii / Q000032:analysis / Q000276:immunology
D008070:Lipopolysaccharides
Uluchshennyĭ metod vydeleniia lipopolisakharidov iz gramotritsatel'nykh bakteriĭ.