title: Comparative study of the clastogenic efficiency of ethyl methanesulfonate and diethyl sulfate in Drosophila melanogaster mature sperm.
abstract: Chromosome loss and translocation tests were carried out in Drosophila melanogaster sperm, stored in untreated females for up to 24 days, to compare the clastogenicity of ethyl methanesulfonate (EMS) and diethyl sulfate (DES). The sex-linked recessive lethal test was used as a "biological dosimeter" and the following results were obtained: The yield of 2-3 translocations induced by both mutagens increased steadily with storage, being significantly higher after EMS than after DES treatment. The frequencies of partial losses induced by EMS and DES were similar and increased with storage. With up to 11 days' storage, the frequency of complete loss induced by DES was higher than that induced by EMS and remained unchanged when storage was extended to 24 days. Complete loss induced by EMS increased significantly with further storage (12-24 days). With DES, complete (but not partial) loss was detected with a dose at which EMS failed to modify the control values. These data suggest that the lower recovery of II-III translocations after treatment with DES does not result from a low breaking capacity but from a diminished or delayed rejoining of the induced breaks. This could be due to a physiological impairment of the treated cells by the high toxicity of DES or to an actual lower rejoinability of the broken ends. The differential recovery of complete and partial losses after DES treatment further suggests that the mechanisms leading to the fixation of both types of damage are somehow different, and that processes intervening in the recovery of partial losses are less affected, or not at all, by the proposed reduced rejoining of chromosome breaks.
mesh_terms: D000782:Aneuploidy; D004249:DNA Damage; D004260:DNA Repair; D004331:Drosophila melanogaster / Q000187:drug effects* / Q000235:genetics; D005020:Ethyl Methanesulfonate / Q000494:pharmacology*; D005804:Genes, Lethal; D005808:Genes, Recessive; D008297:Male; D009152:Mutagenicity Tests; D013094:Spermatozoa / Q000187:drug effects* / Q000648:ultrastructure; D013463:Sulfuric Acid Esters / Q000494:pharmacology*; D013464:Sulfuric Acids / Q000494:pharmacology*; D014178:Translocation, Genetic
chemical_list: D013463:Sulfuric Acid Esters; D013464:Sulfuric Acids; D005020:Ethyl Methanesulfonate; C011612:diethyl sulfate