title: alpha-Difluoromethylornithine does not bind to ornithine decarboxylase-antizyme complex.
abstract: alpha-Difluoromethylornithine (DFMO) has been widely used for determining the amounts of ornithine decarboxylase (ODC) in cells. However, we have now found that DFMO does not react with ODC-antizyme complex, indicating that the amounts of radioactive DFMO bound to the protein may not reflect the amounts of ODC in crude tissue extracts.
mesh_terms: D000818:Animals; D000518:Eflornithine; D046911:Macromolecular Substances; D009952:Ornithine / Q000031:analogs & derivatives* / Q000378:metabolism; D009955:Ornithine Decarboxylase / Q000378:metabolism*; D065108:Ornithine Decarboxylase Inhibitors; D011506:Proteins / Q000378:metabolism*; D051381:Rats
chemical_list: D046911:Macromolecular Substances; D065108:Ornithine Decarboxylase Inhibitors; D011506:Proteins; C031017:ornithine decarboxylase antizyme; D009952:Ornithine; D009955:Ornithine Decarboxylase; D000518:Eflornithine