Rapid identification of NH2-terminal myristyl peptides by reversed-phase high-performance liquid chromatography.
NH2-terminal myristyl peptides in mixtures of other peptides having the NH2-terminal blocked with acetyl, formyl or pyroglutamyl groups were selectively determined by high-performance liquid chromatography using a poly(vinyl alcohol) resin column eluted with 50 mM sodium hydrogen carbonate, pH 8.3, and a linear gradient of acetonitrile. This method was applied to the analysis of an NH2-terminal blocked peptide from a peptic digest of the catalytic subunit of adenosine 3':5'-phosphate-dependent protein kinase type II from bovine heart, showing that the peptide has a sequence myristyl-Gly-Asn-Ala.
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005227:Fatty Acids / Q000302:isolation & purification; D007202:Indicators and Reagents; D046911:Macromolecular Substances; D009206:Myocardium / Q000201:enzymology; D009227:Myristic Acids / Q000032:analysis*; D010434:Pepsin A; D010446:Peptide Fragments / Q000032:analysis; D010455:Peptides / Q000032:analysis*; D011494:Protein Kinases; D013329:Structure-Activity Relationship
D005227:Fatty Acids; D007202:Indicators and Reagents; D046911:Macromolecular Substances; D009227:Myristic Acids; D010446:Peptide Fragments; D010455:Peptides; D011494:Protein Kinases; D010434:Pepsin A