Nonenzymatic glucosylation as a contributing factor to defective fibrinolysis in diabetes mellitus.
Kinetics of activation of plasminogen purified from the plasma of 3 uncontrolled diabetic patients by tissue type plasminogen activator revealed substrate inhibition in the fibrin-stimulated system. After improvement of metabolic parameters activation of plasminogen was found to be normal in 1 of these patients and partially improved in another patient. Studies performed to investigate a possible nonenzymatic glucosylation of plasminogen showed that purified control plasminogen incorporates 14C-glucose in a dose- and time-dependent manner and that in vitro glucosylation of control plasminogen results in functional abnormalities of plasminogen, which resemble those observed with plasminogen from diabetic patients as far as substrate inhibition in the fibrin-stimulated system is concerned.
D002250:Carbon Radioisotopes; D002846:Chromatography, Affinity; D003920:Diabetes Mellitus / Q000097:blood*; D004789:Enzyme Activation; D005342:Fibrinolysis; D005947:Glucose / Q000378:metabolism; D006031:Glycosylation; D006801:Humans; D007700:Kinetics; D010958:Plasminogen / Q000378:metabolism* / Q000502:physiology; D010959:Tissue Plasminogen Activator / Q000494:pharmacology
D002250:Carbon Radioisotopes; D010958:Plasminogen; D010959:Tissue Plasminogen Activator; D005947:Glucose