title: Cloning and transcriptional regulation of the elastase lasA gene in mucoid and nonmucoid Pseudomonas aeruginosa.
abstract: The lasA gene (whose product is involved in the production of extracellular elastolytic activity) was isolated from a genomic bank containing DNA from Pseudomonas aeruginosa FRD1. Recombinant plasmid pELA1, containing the lasA gene, complemented the temperature-sensitive elastase mutation (lasA1) in P. aeruginosa PAO-E64. The lasA gene was physically mapped on plasmid pELA1 by deletion analysis and transposon mutagenesis. The direction of transcription of lasA was determined with a promoterless chloramphenicol acetyltransferase cartridge. The lasA-chloramphenicol acetyltransferase plasmid was transferred to isogenic mucoid and nonmucoid strains of P. aeruginosa FRD; the transcription of lasA-chloramphenicol acetyltransferase was slightly higher in the nonmucoid strain.
mesh_terms: D003001:Cloning, Molecular; D005786:Gene Expression Regulation; D005796:Genes; D005798:Genes, Bacterial; D010196:Pancreatic Elastase / Q000235:genetics* / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000201:enzymology / Q000235:genetics*; D013045:Species Specificity; D014158:Transcription, Genetic
chemical_list: D010196:Pancreatic Elastase