Enzymatic quantification of strand breaks of DNA induced by vacuum-UV radiation.
HindIII-digested plasmid DNA dried on an aluminum plate was irradiated by vacuum-UV at 160 and 195 nm using a synchrotron irradiation system. A change induced in the DNA, presumably a single strand break, was quantified by the aid of the strand break-derived stimulation of poly(ADP-ribose) synthetase activity. The end group of strand breaks so induced was recognized by the enzyme as effectively as that by DNase I treatment, suggesting a nicking as the major lesion inflicted on the DNA. The fluence (UV) dependent stimulation of poly(ADP-ribose) synthetase activity was much higher upon 160 nm irradiation than upon 195 nm irradiation.
D004247:DNA / Q000378:metabolism / Q000528:radiation effects*; D010957:Plasmids; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism*; D014466:Ultraviolet Rays; D014618:Vacuum
D004247:DNA; D011065:Poly(ADP-ribose) Polymerases