title: Relationship of fetal age at conjunctival exposure of pregnant heifers and Brucella abortus isolation.
abstract: Pregnant heifers were exposed by a conjunctival inoculation with 1 X 10(7) colony-forming units of Brucella abortus strain 2308. At parturition, milk and uterine samples from dams plus samples from dead calves were cultured bacteriologically for Brucella. The logistic regression probability of B abortus isolation increased from 0.22 to 0.90, as fetal age at exposure of heifers increased from 60 to 150 gestation days. Strain 2308 was recovered at parturition from 14 (64%) of 22, 17 (71%) of 24, and all 28 (100%) heifers that were at gestation days less than 127, 127 to 157, and greater than 157, respectively, at time of exposure. The number of infected heifers and the number of samples positive for B abortus were significantly increased as fetal age at exposure of heifers increased from gestation days less than 127 to greater than 157 (chi 2 greater than 10, P less than 0.005).
mesh_terms: D000818:Animals; D002003:Brucella abortus / Q000302:isolation & purification*; D002007:Brucellosis, Bovine / Q000635:transmission*; D002417:Cattle; D003234:Conjunctivitis, Bacterial / Q000662:veterinary*; D005260:Female; D005315:Fetal Diseases / Q000382:microbiology / Q000662:veterinary*; D005865:Gestational Age; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000662:veterinary*