Allelic exclusion frequency analysis and molecular characteristics of immunoglobulins secreted by the hybridomas expressing both allelic genes.
The investigation of 750 B-lymphocyte hybridoma clones obtained by fusion of mouse myeloma and newborn heterozygous Igk-1a/Igk-1b rat splenocytes has revealed that 9.8% of Ig kappa-chain loci are rearranged productively. Seventeen hybridomas secrete kappa-chains of both allelic variants. The analysis of IgM molecules of 9 such clones demonstrated that in 6 cases only one L-chain allotype, either 1a or 1b, is present in IgM. Thus for the first time the high frequency of selective association of H and L chains in Ig-producing cells was shown. Evidently this selectivity may function as one of allelic exclusion mechanisms at the Ig assembly stage.
D000818:Animals; D001402:B-Lymphocytes / Q000502:physiology*; D005786:Gene Expression Regulation; D006825:Hybridomas / Q000502:physiology*; D007126:Immunoglobulin Allotypes / Q000235:genetics*; D007145:Immunoglobulin kappa-Chains / Q000235:genetics*; D051379:Mice; D051381:Rats; D011995:Recombination, Genetic
D007126:Immunoglobulin Allotypes; D007145:Immunoglobulin kappa-Chains