Start of mu-chain production by the further two-step rearrangements of immunoglobulin heavy chain genes on one chromosome from a DJH/DJH configuration in an Abelson virus-transformed cell line: evidence of secondary DJH complex formation.
A variant was selected from an Abelson virus-transformed cell line with identifiable and distinguishable DJH rearrangements on both chromosomes. Southern blotting experiments suggested that this variant resulted from a secondary DJH complex formation accompanied with the deletion of one of the DJH rearrangements on the same chromosome. Furthermore, this variant started making mu-chains by the further functional join of a VH gene to the secondarily formed DJH complex. Thus, we describe here the first evidence of a secondary DJH complex formation.
D000011:Abelson murine leukemia virus; D001402:B-Lymphocytes / Q000502:physiology*; D002472:Cell Transformation, Viral; D003593:Cytoplasm / Q000032:analysis; D005796:Genes; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D007148:Immunoglobulin mu-Chains / Q000235:genetics*; D011995:Recombination, Genetic; D013997:Time Factors
D007135:Immunoglobulin Variable Region; D007148:Immunoglobulin mu-Chains