Determination of the complete amino acid sequence of recombinant human gamma-interferon produced in Escherichia coli.
The complete amino acid sequence of recombinant human gamma-interferon (HuIFN-gamma) produced in Escherichia coli was determined using a gas-phase protein sequencer. The sequence was established by automated Edman degradation on the intact protein and its peptides obtained after Staphylococcus aureus V8 protease or trypsin digestion. The result was identical to the amino acid sequence predicted from the nucleotide sequence of the cloned HuIFN-gamma cDNA except that it was missing the four carboxy-terminal residues, Arg-Ala-Ser-Gln.
D000595:Amino Acid Sequence; D004247:DNA / Q000235:genetics; D004926:Escherichia coli; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics*; D011994:Recombinant Proteins / Q000235:genetics*
D011994:Recombinant Proteins; D007371:Interferon-gamma; D004247:DNA