Arachidonic acid metabolites during nasal challenge.
In order to assess the role of arachidonic acid metabolites in the early reaction to antigen, we challenged six allergic individuals with and without premedication with aspirin and recorded their clinical response, as indicated by number of sneezes, and measured the levels of inflammatory mediators. The early reaction to antigen was associated with increases in the levels of histamine, N-alpha-tosyl-L-arginine methyl esterase (TAME-esterase) activity, prostaglandin (PG) D2, leukotriene C4, PGE, and thromboxane. Aspirin significantly inhibited the increases in the cyclooxygenase metabolites PGE, PGD2, PGF2 alpha, 6-keto-PGF1 alpha, and thromboxane but did not affect the amount of sneezing or the levels of histamine, TAME-esterase activity, or leukotrienes. The pattern of the metabolites and their response to pretreatment with aspirin parallel the response of purified human lung mast cells, supporting the notion that the early phase of allergic rhinitis is a mast cell-dominated event.
D015121:6-Ketoprostaglandin F1 alpha / Q000378:metabolism; D000328:Adult; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism*; D001241:Aspirin / Q000627:therapeutic use; D005260:Female; D006632:Histamine / Q000378:metabolism; D006801:Humans; D008297:Male; D009299:Nasal Provocation Tests; D010447:Peptide Hydrolases / Q000378:metabolism; D011292:Premedication; D015230:Prostaglandin D2; D011457:Prostaglandins D / Q000378:metabolism; D011458:Prostaglandins E / Q000378:metabolism; D006255:Rhinitis, Allergic, Seasonal / Q000175:diagnosis* / Q000378:metabolism; D013189:SRS-A / Q000378:metabolism; D012912:Sneezing; D013931:Thromboxanes / Q000378:metabolism
D001095:Arachidonic Acids; D011457:Prostaglandins D; D011458:Prostaglandins E; D013189:SRS-A; D013931:Thromboxanes; D016718:Arachidonic Acid; D015121:6-Ketoprostaglandin F1 alpha; D006632:Histamine; D010447:Peptide Hydrolases; C023073:tosylarginine methyl ester hydrolase; D001241:Aspirin; D015230:Prostaglandin D2