title: Preliminary investigations of a correlation between electron energy loss and morphometric analyses on ultrathin cryosections from normal and neoplastic gastric tissues.
abstract: Electron energy loss spectroscopy (EELS) has been used to measure the ratios of C, N, O, P and Ca in ultrathin cryosections from normal and neoplastic gastric tissues. First results show a correlation between the EELS, and morphometric data in cells from these tissues. We have found that ultrathin, freeze-dried cryosections, with an average thickness of up to 75 nm, are stable enough for EELS-analysis in a 200 KV electron microscope with an adapted Gatan-EELS-Spectrometer.
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism* / Q000473:pathology / Q000648:ultrastructure; D002118:Calcium / Q000378:metabolism; D002244:Carbon / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D004583:Electrons; D004734:Energy Metabolism; D005756:Gastritis / Q000378:metabolism / Q000473:pathology; D006652:Histological Techniques; D006801:Humans; D008854:Microscopy, Electron; D009584:Nitrogen / Q000378:metabolism; D010758:Phosphorus / Q000378:metabolism; D013057:Spectrum Analysis / Q000379:methods*; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000473:pathology / Q000648:ultrastructure; D014018:Tissue Distribution
chemical_list: D010758:Phosphorus; D002244:Carbon; D009584:Nitrogen; D002118:Calcium