title: Delayed-amelanotic (DAM or Smyth) chicken: melanocyte dysfunction in vivo and in vitro.
abstract: Chickens of the autoimmune delayed-amelanotic (DAM or Smyth) line develop postnatal feather amelanosis and severe visual defects, both of which are presumed to be due to a dysfunction of melanocytes and a subsequent autoimmune response that eliminates pigment cells. In this report we elucidate further the melanocytic defect. We present a morphologic analysis of the mildly affected erratic (eDAM) group of Smyth chicken whose partial depigmentation and lack of visual impairment resemble human vitiligo more so than do the complete amelanosis and blindness in the classical Smyth line. Histologically, the sequential events leading to amelanosis in the young Smyth chicken occur simultaneously in the feathers of adult eDAM Smyth chickens, and the infiltration of the feather pulp with mononuclear leukocytes correlates with the extent of local pigmentary abnormality. Cytochemical localizations of dopa-oxidase and acid-phosphatase activities in eDAM feather melanocytes suggest that melanogenesis and autophagocytosis of melanosomes occur in tandem and that the rates of both are higher in these cells than in melanocytes of normally pigmented control chickens. Assays for tyrosinase activity in feather follicles indicate a hypermelanization in eDAM feathers and in the pigmented feathers of young Smyth chicks prior to the onset of depigmentation. Finally, we report on the establishment of pure, proliferative cultures of neural crest-derived melanocytes from control and Smyth chicken embryos. The degenerative events in Smyth chicken melanocyte cultures mimic in part those of the cells in vivo and are therefore indicative of a genetic defect that is independent of the immune system.
mesh_terms: D000818:Animals; D001327:Autoimmune Diseases / Q000662:veterinary*; D002478:Cells, Cultured; D002642:Chick Embryo; D002645:Chickens; D004295:Dihydroxyphenylalanine / Q000032:analysis; D004195:Disease Models, Animal; D005241:Feathers / Q000502:physiology / Q000648:ultrastructure; D008544:Melanocytes / Q000502:physiology* / Q000648:ultrastructure; D014442:Monophenol Monooxygenase / Q000032:analysis; D010587:Phagocytosis; D010859:Pigmentation Disorders / Q000662:veterinary*; D011201:Poultry Diseases / Q000276:immunology / Q000473:pathology*; D012038:Regeneration; D014820:Vitiligo / Q000276:immunology / Q000473:pathology*
chemical_list: D004295:Dihydroxyphenylalanine; D014442:Monophenol Monooxygenase