Extinction of expression of immunoglobulin genes in myeloma X fibroblast somatic cell hybrids.
Adherent hybrids between immunoglobulin-producing mouse myeloma cells and fibroblasts do not produce immunoglobulin polypeptide chains. These hybrids retained the actively rearranged immunoglobulin genes of the myeloma parental cells but lacked immunoglobulin heavy- and light-chain RNA transcripts. We conclude that the shutoff of immunoglobulin production in these hybrids occurs at the transcription or early processing level.
D000818:Animals; D005347:Fibroblasts / Q000502:physiology; D005786:Gene Expression Regulation; D006822:Hybrid Cells / Q000502:physiology*; D007142:Immunoglobulin gamma-Chains / Q000235:genetics; D007145:Immunoglobulin kappa-Chains / Q000235:genetics; D007136:Immunoglobulins / Q000235:genetics*; D051379:Mice; D010954:Plasmacytoma / Q000503:physiopathology; D012333:RNA, Messenger / Q000235:genetics; D014158:Transcription, Genetic
D007142:Immunoglobulin gamma-Chains; D007145:Immunoglobulin kappa-Chains; D007136:Immunoglobulins; D012333:RNA, Messenger