Biosynthesis of thiamin. Precursor of C-5, C-6, and hydroxymethyl carbon atoms of the pyrimidine moiety in a eucaryote.
We studied the incorporation of radioactive glucose into the pyrimidine moiety of thiamin in the eucaryote Candida utilis. Three carbons of glucose were incorporated into the pyrimidine, and the C-2 of glucose into the C-6 of the pyrimidine. We concluded that the C-5, -6, and hydroxymethyl carbon atoms of the pyrimidine in this eucaryote originate from the C-2, -3 and -4 of glucose via ribose.
D002175:Candida / Q000378:metabolism*; D002250:Carbon Radioisotopes; D005561:Formates / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D005998:Glycine / Q000378:metabolism; D012266:Ribose / Q000378:metabolism; D013831:Thiamine / Q000096:biosynthesis*
D002250:Carbon Radioisotopes; D005561:Formates; C030544:formic acid; D012266:Ribose; D005947:Glucose; D005998:Glycine; D013831:Thiamine