Enzymatic determination of isocitrate by amperometric monitoring of the rate of oxygen consumption.
A coupled enzymatic method elaborated for NAD+-dependent dehydrogenases has been adapted for NADP+-dependent isocitrate dehydrogenase, in combination with amperometric measurements. The isocitrate dehydrogenase activity dependent linearly on the isocitrate concentration in the range 0-2 X 10(-4) M. Application of this method affords a sensitive estimation of isocitrate even in turbid liquids such as fermentation broths.
D004566:Electrodes; D007521:Isocitrate Dehydrogenase; D007523:Isocitrates / Q000032:analysis*; D010101:Oxygen Consumption
D007523:Isocitrates; C034219:isocitric acid; D007521:Isocitrate Dehydrogenase