title: [Serological activity of anti-Brucella abortus immunoglobulins in the rose bengal plate test in cattle].
abstract: The present study was to determine serological activity of cattle anti-Brucella antibodies in rose bengal plate test with regard to classical tests, i.e. standard agglutination test (SAT) and CFT (Complement Fixation Test). The serum containing antibodies of IgM class characteristic of the early stage of infection was taken from a calf on 13th day after the administration of Br. abortus S19 killed strain. The serum containing IgG antibodies (IgG1 and IgG2 subclasses) indicating the chronic brucellosis was taken from a cow infected naturally. IgM antibodies were isolated from serum by column chromatography on Sefadeks G-200 gel and IgG1 and IgG2 by column chromatography on DEAE cellulose. It was found out that IgM, IgG1, IgG2 were active in RBPT, SAT and IgM, IgG1 in CFT. The activity of anti-Brucella immunoglobulins in RBPT proves the possibility of diagnosing the above mentioned infections using this test. Taking into account titer height, in particular tests with certain Ig class, it can be said that acidic medium in RBPT reduces not selectively the activity of all immunoglobulin classes comparing with SAT and CFT, and that RBPT is typical qualitative test more valuable than SAT. The reduction of activity of all immunoglobulin classes in RBPT at significantly lower content of M immunoglobulin than IgG1 in the serum may explain the very high specificity of the test. Moreover, it was found that half the amount of the antigen in RBPT has a significant effect on the increase of test sensitivity.
mesh_terms: D000372:Agglutination Tests / Q000662:veterinary; D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis*; D002003:Brucella abortus / Q000276:immunology*; D002007:Brucellosis, Bovine / Q000175:diagnosis* / Q000276:immunology; D002417:Cattle; D007136:Immunoglobulins / Q000032:analysis*; D012395:Rose Bengal
chemical_list: D000907:Antibodies, Bacterial; D007136:Immunoglobulins; D012395:Rose Bengal
vernacular_title: Aktywność serologiczna bydlecych immunoglobulin anty-Brucella abortus w odczynie kwaśnej aglutynacji płytowej (OKAP).