Induction of cytochrome P-450 in Streptomyces griseus by soybean flour.
Soybean flour and the isoflavonoid genistein, were found to induce cytochrome P-450 in Streptomyces griseus. The chromophore was found in the 105,000xg supernatant and gave a reduced CO-difference spectrum with an absorption maximum of 448 nm. Almost 70% of the P-450 could be precipitated at 35-45% ammonium sulfate saturation. SDS-gel electrophoresis revealed the presence of a 45,000 dalton polypeptide in extracts induced by either soybean or genistein. S. griseus generated the free isoflavonoids genistein and daidzein when grown on soybean flour medium.
D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis* / Q000302:isolation & purification; D004790:Enzyme Induction / Q000187:drug effects; D005433:Flour; D019833:Genistein; D007529:Isoflavones / Q000494:pharmacology; D013025:Glycine max; D013057:Spectrum Analysis; D013305:Streptomyces griseus / Q000201:enzymology*
D007529:Isoflavones; D003577:Cytochrome P-450 Enzyme System; D019833:Genistein