Peptides with antilipolytic and lipogenic activities from seeds of the bitter gourd Momordica charantia (family Cucurbitaceae).
Decorticated Momordica charantia seeds were extracted with acidic ethanol. The pH of the extract was adjusted to 8 and the resulting precipitate was chromatographed on CM Sepharose CL-6B and then desalted on Sephadex G-10. A number of factors which exhibited antilipolytic and lipogenic activities in rat adipocytes were isolated. Two of the factors were demonstrated to be peptides with similar amino acid compositions and a molecular weight of approximately 8000. However the peptides greatly differed in their chromatographic behavior on CM-Sepharose CL-6B and in their mobilities in agarose and polyacrylamide gel electrophoresis.
D000020:Abortifacient Agents, Nonsteroidal / Q000302:isolation & purification; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002850:Chromatography, Gel; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006386:Hemagglutination Tests; D006461:Hemolysis / Q000187:drug effects; D000960:Hypolipidemic Agents / Q000302:isolation & purification*; D005779:Immunodiffusion; D008055:Lipids / Q000096:biosynthesis*; D051379:Mice; D008813:Mice, Inbred ICR; D008970:Molecular Weight; D009699:N-Glycosyl Hydrolases; D010455:Peptides / Q000302:isolation & purification / Q000494:pharmacology*; D010940:Plant Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D054790:Ribosome Inactivating Proteins, Type 2; D012639:Seeds / Q000032:analysis; D012967:Sodium Dodecyl Sulfate
D000020:Abortifacient Agents, Nonsteroidal; D000596:Amino Acids; D000960:Hypolipidemic Agents; D008055:Lipids; D010455:Peptides; D010940:Plant Proteins; D054790:Ribosome Inactivating Proteins, Type 2; D012967:Sodium Dodecyl Sulfate; D009699:N-Glycosyl Hydrolases