Differential effects of in vitro gold sodium thiomalate on the stimulation of human peripheral blood mononuclear cells by Mycoplasma arthritidis T cell mitogen, concanavalin A and phytohemagglutinin.
The effects of in vitro gold sodium thiomalate (GSTM) on peripheral blood mononuclear cells (PBM) stimulated by a Mycoplasma arthritidis T cell mitogen (MAM), concanavalin A (Con A), and phytohemagglutinin (PHA) were compared in 5 individuals by measuring 3H-thymidine uptake by PBM. The MAM induced lymphocyte transformations were more significantly inhibited by in vitro GSTM than parallel assays using Con A and PHA. The effects could not be explained by differences in cell viability, length of cell culture, mitogen concentration, and level of mitogen stimulation, suggesting that MAM may be a useful probe in evaluating the effects of GSTM on immune function.
D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003208:Concanavalin A / Q000494:pharmacology*; D006052:Gold Sodium Thiomalate / Q000494:pharmacology*; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects; D008934:Mitogens / Q000494:pharmacology*; D009000:Monocytes / Q000166:cytology / Q000187:drug effects*; D010835:Phytohemagglutinins / Q000494:pharmacology*; D013268:Stimulation, Chemical
D008934:Mitogens; D010835:Phytohemagglutinins; D003208:Concanavalin A; D006052:Gold Sodium Thiomalate