title: Cloning of a cDNA encoding a surface antigen of Schistosoma mansoni schistosomula recognized by sera of vaccinated mice.
abstract: Spleen cells of mice vaccinated with radiation-attenuated Schistosoma mansoni cercariae were used to produce monoclonal antibodies directed against newly transformed schistosomular surface antigens. One of these monoclonal antibodies recognized a polypeptide of 18 kDa. This glycoprotein was purified by monoclonal antibody immunoaffinity chromatography and a polyclonal antiserum was prepared against it. Immunofluorescence assays showed that the polyclonal antiserum bound to the surface of newly transformed schistosomula and lung-stage organisms but not to the surface of liver-stage and adult worms. Using this polyclonal antiserum we isolated recombinant clones from an adult worm cDNA expression library constructed in lambda gt11. Clone 654.2 contained an insert of 0.52 kilobase and hybridized to a 1.2-kilobase mRNA species from adult worms. Most importantly, clone 654.2 produced a fusion protein of 125 kDa that was reactive with sera of vaccinated mice that are capable of transferring resistance. This result encourages future vaccination trials with the fusion protein.
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D000947:Antigens, Helminth / Q000235:genetics* / Q000302:isolation & purification; D000954:Antigens, Surface / Q000235:genetics* / Q000302:isolation & purification; D002846:Chromatography, Affinity; D003001:Cloning, Molecular; D006224:Cricetinae; D004247:DNA / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D005796:Genes; D008647:Mesocricetus; D051379:Mice; D012333:RNA, Messenger / Q000235:genetics; D012550:Schistosoma mansoni / Q000235:genetics* / Q000276:immunology; D012555:Schistosomiasis mansoni / Q000276:immunology*; D014158:Transcription, Genetic; D014611:Vaccination
chemical_list: D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D000947:Antigens, Helminth; D000954:Antigens, Surface; D012333:RNA, Messenger; D004247:DNA