Purification of two species of exudate cysteine-proteinase inhibitors that are acute-phase reactants in the carrageenin-induced inflammation in rats.
Two species of cysteine-proteinase inhibitors (CPIs) have been purified to homogeneity from exudate in the carrageenin-induced inflammation in rats. The exudate CPIs were separated into two forms (named CPI-1 and -2) in affinity chromatography on S-carboxymethyl-papain-Sepharose, the final stage of purification. CPI-1 and -2 gave different mobilities in polyacrylamide gel electrophoresis (PAGE), probably because of different isoelectric points (pI 4.47 for CPI-1 and pI 4.21 for CPI-2). Both CPI-1 and -2 showed immunological identity in double immunodiffusion and same molecular mass of 68 kDa when analysed by SDS-PAGE. These results indicate that CPI-1 and -2 are very similar but distinct CPIs. CPI-1 and -2 are acute-phase reactants and probably represent two species of T-kininogens having inhibitory activity toward cysteine proteinases.
D000210:Acute-Phase Reaction / Q000378:metabolism*; D000818:Animals; D002351:Carrageenan / Q000494:pharmacology; D015853:Cysteine Proteinase Inhibitors; D004591:Electrophoresis, Polyacrylamide Gel; D005122:Exudates and Transudates / Q000032:analysis*; D007249:Inflammation / Q000378:metabolism*; D007704:Kininogens / Q000032:analysis; D008297:Male; D008970:Molecular Weight; D011506:Proteins / Q000302:isolation & purification*; D051381:Rats; D011919:Rats, Inbred Strains
D015853:Cysteine Proteinase Inhibitors; D007704:Kininogens; D011506:Proteins; D002351:Carrageenan