title: Monoclonal antibodies against acetylcholinesterase from electric organs of Electrophorus and Torpedo.
abstract: We studied the reactivity of monoclonal antibodies (mAbs) raised against acetylcholinesterase (AChE) purified from Electrophorus and Torpedo electric organs. We obtained IgG antibodies (Elec-21, Elec-106, Tor-3E5, Tor-ME8, Tor-1A5), all of them directed against the catalytic subunit of the corresponding species, with no significant cross-reactivity. These antibodies do not inhibit the enzyme and recognize all molecular forms, globular (G) and asymmetric (A). Tor-ME8 reacts specifically with the denatured A and G subunits of Torpedo AChE, in immunoblots. Several hybridomas raised against Electrophorus AChE produced IgM antibodies (Elec-39, Elec-118, Elec-121). These antibodies react with the A forms of Electrophorus electric organs and also with a subset of dimers (G2) from Torpedo electric organ. In addition, they react with a number of non-AChE components, in immunoblots. In contrast, they do not recognize AChE from other Electrophorus tissues or A forms from Torpedo electric organs.
mesh_terms: D000110:Acetylcholinesterase / Q000032:analysis* / Q000276:immunology; D000818:Animals; D000911:Antibodies, Monoclonal; D002091:Butyrylcholinesterase / Q000276:immunology; D002499:Centrifugation, Density Gradient; D003101:Collodion; D003429:Cross Reactions; D004557:Electric Organ / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel; D004593:Electrophorus; D007124:Immunoenzyme Techniques; D011863:Radioimmunoassay; D014101:Torpedo
chemical_list: D000911:Antibodies, Monoclonal; D003101:Collodion; D000110:Acetylcholinesterase; D002091:Butyrylcholinesterase