Identification of mammalian feces by thin layer chromatography of coprostanol: collaborative study.
Mammalian feces contain coprostanol (5 beta-cholestan-3 beta-ol). In this study, 7 collaborators each tested 45 unknown specimens by a thin layer chromatographic method that uses coprostanol as an indicator of feces. The materials tested were 5 replicates each of 3 test portion sizes (0.5, 1.0, and 5.0 mg) of cockroach excreta (negative), and cow and rat feces (both positive). Of 315 specimens tested, 261 (82.9%) were correctly identified; there were 5 false positives, 26 false negatives, and from 1 collaborator, 23 inconclusive results.
D000818:Animals; D001717:Birds / Q000378:metabolism; D002417:Cattle; D004083:Cholestanol / Q000032:analysis*; D002784:Cholesterol / Q000031:analogs & derivatives*; D002855:Chromatography, Thin Layer; D003058:Cockroaches / Q000378:metabolism; D005243:Feces / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis; D007202:Indicators and Reagents; D008322:Mammals / Q000378:metabolism*; D051381:Rats
D007202:Indicators and Reagents; D004083:Cholestanol; D002784:Cholesterol