Volume regulation in leukemic and lymphoma cells in children and determination of cell lineage.
Among normal lymphocytes, T cells can readjust their volume rapidly following initial swelling in a hypotonic medium, whereas B cells do not have this ability. Based on this finding, we examined the volume regulation of malignant cells from 40 patients with lymphocytic and nonlymphocytic malignancies. The T lineage cells were able to regulate their volume in response to hypotonic stress, whereas B lineage cells were not able to do so. In contrast to lymphoid lineage cells, nonlymphocytic leukemia cells as well as undifferentiated cells did not show a consistent tendency in their volume regulation. These results showed that the difference in the ability to regulate cell volume in response to hypotonic stress is available as a marker for identifying the cellular lineage of lymphoid malignancies.
D000208:Acute Disease; D000328:Adult; D000911:Antibodies, Monoclonal; D001402:B-Lymphocytes / Q000473:pathology; D002648:Child; D002675:Child, Preschool; D006801:Humans; D007938:Leukemia / Q000145:classification / Q000276:immunology / Q000473:pathology*; D008223:Lymphoma / Q000145:classification / Q000276:immunology / Q000473:pathology*; D009504:Neutrophils / Q000473:pathology; D010641:Phenotype; D013601:T-Lymphocytes / Q000473:pathology
D000911:Antibodies, Monoclonal