Mitomycin-C is an unreliable inhibitor for study of DNA synthesis in Plasmodium.
Cytophotometric studies on DNA synthesis during asexual and sexual development of Plasmodium berghei contradicted earlier conclusions on DNA synthesis in Plasmodium which were largely based on experiments in which mitomycin-C had been used as a DNA replication inhibitor. Therefore, the effect of mitomycin on intra erythrocytic asexual development and on microgametogenesis, fertilization and zygote/ookinete development of P. berghei was studied in vitro. All DNA-synthesizing stages (schizonts, exflagellating microgametocytes and zygotes) and also DNA synthesis itself in all such stages, are totally unaffected by mitomycin concentrations 10 times higher than that which inhibits normal development of the non-DNA-synthesizing rings and trophozoites. The results are explained by the mode of action of mitomycin.
D000818:Animals; D003592:Cytophotometry; D004247:DNA / Q000096:biosynthesis*; D005306:Fertilization / Q000187:drug effects; D005718:Gametogenesis / Q000187:drug effects; D016685:Mitomycin; D008937:Mitomycins / Q000494:pharmacology*; D010962:Plasmodium berghei / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism
D008937:Mitomycins; D016685:Mitomycin; D004247:DNA