title: Molecular cloning of an amylase gene of Bacillus circulans.
abstract: An amylase gene of Bacillus circulans was cloned in B. subtilis and its nucleotide sequence was determined. The putative proamylase consists of 528 amino acids, which correspond to a molecular weight of 58,776. Homologous regions with other amylases of Bacillus species were found. A sigma 55-type promoter is located at about 250 bp upstream from the starting codon. This promoter was also functional in Escherichia coli, and able to express beta-galactosidase activity.
mesh_terms: D000595:Amino Acid Sequence; D001407:Bacillus / Q000201:enzymology / Q000235:genetics*; D001412:Bacillus subtilis / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D003001:Cloning, Molecular; D004790:Enzyme Induction; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D013213:Starch / Q000378:metabolism; D000516:alpha-Amylases / Q000235:genetics* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D011993:Recombinant Fusion Proteins; D013213:Starch; D000516:alpha-Amylases