An ELISA that detects cell-associated and released rat IL-2 receptors in a soluble form.
A mouse anti-rat interleukin-2 receptor (IL-2R) monoclonal antibody (mAb), ART-65, has recently been developed which recognizes the rat IL-2R but binds to an epitope distinct from that recognized by the mAb ART-18. The availability of two mAb against the rat IL-2R molecule permitted the development of an enzyme-linked immunosorbent assay (ELISA) which detects soluble and solubilized rat IL-2R. Using this ELISA, time course studies of ConA and LPS-activated rat splenocytes revealed that IL-2R are released into their environment during the culture period, and that IL-2R can be detected in rat sera. The significance of the results is discussed.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D003208:Concanavalin A / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D008070:Lipopolysaccharides / Q000494:pharmacology; D051381:Rats; D011917:Rats, Inbred Lew; D011971:Receptors, Immunologic / Q000032:analysis*; D015375:Receptors, Interleukin-2; D013154:Spleen / Q000032:analysis; D013997:Time Factors
D000911:Antibodies, Monoclonal; D008070:Lipopolysaccharides; D011971:Receptors, Immunologic; D015375:Receptors, Interleukin-2; D003208:Concanavalin A