Benoxaprofen photosensitization of phospholipase activation in mammalian cells in culture.
Benoxaprofen (BPF) induces a clinical photosensitivity which resembles idiopathic solar urticaria. This response is mediated by degranulation of mast cells. Since mast-cell degranulation is known to be modulated by phospholipid alteration, we examined the effect of BPF and ultraviolet radiation (UV) on phospholipid metabolism of C3H 10 T1/2 cells in culture. BPF + UVB (280-320 nm) induced release of [3H]arachidonic acid (AA) but did not alter the release of [3H]choline from prelabelled cells. This suggests that BPF photosensitizes phospholipase A2 activation. Such activation probably represents an integral step in the mechanism of BPF photosensitization of mast-cell degranulation.
D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism; D002794:Choline / Q000378:metabolism; D066298:In Vitro Techniques; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D051379:Mice; D010740:Phospholipases / Q000378:metabolism*; D011422:Propionates / Q000633:toxicity*; D014466:Ultraviolet Rays / Q000009:adverse effects*
D001095:Arachidonic Acids; D011422:Propionates; C011677:benoxaprofen; D016718:Arachidonic Acid; D010740:Phospholipases; D002794:Choline