[Characteristics of the precipitation of the antigenic structures of the tick-borne encephalitis virus using polyethylene glycol and ammonium sulfate].
When immunoelectrophoretic methods were used for analysis of tick-borne encephalitis (TBE) virus antigens, TBE virus virion antigen (VA) from the virus-containing tissue culture fluid sedimented upon addition of polyethylene glycol (PEG) up to 7% whereas upon addition of ammonium sulphate (AS) the bulk of VA sedimented in the range of 40-70% salt saturation. The non-virion antigens (NA) sedimented at all PEG (up to 22%) and AS (up to 70% saturation) concentrations. In stringent ultracentrifugation procedure (12-13 X 10(6) g X min), the bulk of VA and a small portion of NA are pelleted. Repeated ultracentrifugation results in sedimentation of additional amounts of VA and NA. The sedimentable NA are pelleted by low PEG (up to 5-7%) and AS (up to 20-30%) concentrations and differ in their interaction with antibodies in the course of immunoelectrophoresis from nonprecipitatable ("soluble") NA. Combined sedimentation of antigens with AS (in the range of 40-70% saturation), intensive dialysis and subsequent sedimentation with low and high PEG concentrations produces homogeneous antigenic preparations containing VA or "soluble" NA.
D000645:Ammonium Sulfate; D000818:Animals; D000956:Antigens, Viral / Q000032:analysis / Q000302:isolation & purification*; D011232:Chemical Precipitation; D004591:Electrophoresis, Polyacrylamide Gel; D004669:Encephalitis Viruses, Tick-Borne / Q000276:immunology / Q000302:isolation & purification*; D006386:Hemagglutination Tests; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D011092:Polyethylene Glycols; D014764:Viral Proteins / Q000032:analysis; D014776:Virus Cultivation
D000956:Antigens, Viral; D014764:Viral Proteins; D011092:Polyethylene Glycols; D000645:Ammonium Sulfate
Kharakteristiki osazhdeniia antigennykh struktur virusa kleshchevogo éntsefalita poliétilenglikolem i sul'fatom ammoniia.