Immunochemical study of role of chargerin II, a product of URFA6L of mitochondrial DNA in energy transduction of rat liver mitochondria.
Antibody against chargerin II [product of the unidentified reading frame A6L (URFA6L) of mitochondrial DNA] inhibited the ATP-Pi exchange reaction and reversed electron flow from succinate to NAD in mitoplasts (inner membrane plus matrix). The antibody against chargerin II caused greater inhibition on incubation with mitoplasts in the energized than the nonenergized state, suggesting that redox reactions are coupled with a conformational change of chargerin II. The present findings showed that chargerin II, the URFA6L product, may have a key role in energy transduction of mitochondrial oxidative phosphorylation.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D000906:Antibodies; D000936:Antigen-Antibody Complex; D004272:DNA, Mitochondrial / Q000235:genetics*; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D007700:Kinetics; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D008930:Mitochondria, Liver / Q000378:metabolism*; D010085:Oxidative Phosphorylation; D010101:Oxygen Consumption; D010710:Phosphates / Q000378:metabolism; D051381:Rats
D000906:Antibodies; D000936:Antigen-Antibody Complex; D004272:DNA, Mitochondrial; D008565:Membrane Proteins; D010710:Phosphates; C044593:chargerins; D000255:Adenosine Triphosphate