Stimulation of IgE sensitized human alveolar macrophages by anti-IgE is unaffected by sodium cromoglycate.
Sodium cromoglycate is an effective prophylactic in the treatment of asthma. However, its mechanism of action is still uncertain. The release of thromboxane B2 and N-acetyl-beta-D-glucosaminidase activity was measured from human alveolar macrophages sensitized with human myeloma IgE. Cells were challenged with rabbit affinity purified anti-IgE (no activity against IgG) in the absence or presence of sodium cromoglycate (10(-4)-10(-8)M). There was no change in the release of either thromboxane B2 or N-acetyl-beta-D-glucosaminidase in the presence of sodium cromoglycate at these concentrations.
D000118:Acetylglucosaminidase / Q000378:metabolism; D000888:Antibodies, Anti-Idiotypic / Q000494:pharmacology*; D000936:Antigen-Antibody Complex / Q000276:immunology; D002478:Cells, Cultured; D004205:Cromolyn Sodium / Q000494:pharmacology*; D006801:Humans; D007114:Immunization; D007073:Immunoglobulin E / Q000276:immunology* / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects* / Q000201:enzymology / Q000276:immunology / Q000378:metabolism; D011650:Pulmonary Alveoli / Q000166:cytology*; D013268:Stimulation, Chemical; D007507:Therapeutic Irrigation; D013929:Thromboxane B2 / Q000378:metabolism
D000888:Antibodies, Anti-Idiotypic; D000936:Antigen-Antibody Complex; C026408:anti-IgE antibodies; D007073:Immunoglobulin E; D013929:Thromboxane B2; D000118:Acetylglucosaminidase; D004205:Cromolyn Sodium