Hemopoietic colony formation by mouse spleen cells in serum-free culture supported by purified erythropoietin and/or interleukin-3.
We examined colony formation by mouse spleen cells in a serum-free culture system containing purified erythropoietin (Ep), interleukin-3 (IL-3) or a combination of the two factors. Purified Ep supported erythroid colony and burst formation but did not support formation of other types of colonies. IL-3 alone did not support erythroid burst formation in culture. However, IL-3 significantly increased the number and size of erythroid bursts. IL-3 but not Ep supported megakaryocyte colony formation. Ep did not enhance megakaryocyte colony formation or megakaryocyte populations in multilineage colonies supported by IL-3.
D000818:Animals; D003114:Colony-Forming Units Assay; D003470:Culture Media; D004921:Erythropoietin / Q000494:pharmacology*; D005472:Fluorouracil / Q000494:pharmacology; D006410:Hematopoiesis / Q000187:drug effects*; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000187:drug effects; D007377:Interleukin-3; D008222:Lymphokines / Q000494:pharmacology*; D008533:Megakaryocytes / Q000166:cytology / Q000187:drug effects; D051379:Mice; D013154:Spleen / Q000166:cytology / Q000187:drug effects
D003470:Culture Media; D007377:Interleukin-3; D008222:Lymphokines; D004921:Erythropoietin; D005472:Fluorouracil