title: Applications of chemical cleavage procedures to the peptide mapping of neurofilament triplet protein bands in sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
abstract: A procedure for examining possible sequence homology in the triplet neurofilament proteins using a sodium dodecyl sulfate-polyacrylamide gel electrophoresis system is described. Five different chemical reagents (cyanogen bromide, BNPS-skatole, hydroxylamine, formic acid, and nitrothiocyanobenzoic acid) have been used for peptide mapping studies. Potential applications of this technique are discussed.
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D003488:Cyanogen Bromide; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005561:Formates; D019811:Hydroxylamine; D006898:Hydroxylamines; D007381:Intermediate Filament Proteins / Q000302:isolation & purification*; D016900:Neurofilament Proteins; D010446:Peptide Fragments / Q000302:isolation & purification*; D012862:Skatole / Q000031:analogs & derivatives; D012967:Sodium Dodecyl Sulfate; D013861:Thiocyanates
chemical_list: D005561:Formates; D006898:Hydroxylamines; D007381:Intermediate Filament Proteins; D016900:Neurofilament Proteins; D010446:Peptide Fragments; D013861:Thiocyanates; C030544:formic acid; C005904:BNPS-skatole; D019811:Hydroxylamine; D012967:Sodium Dodecyl Sulfate; D012862:Skatole; C008658:2-nitro-5-thiocyanobenzoic acid; D003488:Cyanogen Bromide