In vitro RNA synthesis by viruses isolated from the yeast Yarrowia lipolytica.
Viruses isolated from the yeast Yarrowia lipolytica possess a DNA-independent RNA polymerase activity which is inhibited by ethidium bromide and by sodium pyrophosphate but not by actinomycin D. RNA synthesis is maximum at pH 8.0 and at 30 degrees C. Newly synthesized RNA molecules are largely released from the particles, are single-stranded and are able to hybridize with denatured viral genomic RNA.
D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D007700:Kinetics; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000096:biosynthesis*; D013696:Temperature; D014158:Transcription, Genetic; D014771:Virion / Q000235:genetics; D014780:Viruses / Q000201:enzymology / Q000235:genetics*; D015003:Yeasts / Q000201:enzymology / Q000235:genetics*
D012367:RNA, Viral; D012321:DNA-Directed RNA Polymerases