title: The hypothalamus receives major projections from the tuberomammillary nucleus in rat.
abstract: Projections from the tuberomammillary nucleus (TM) to widespread regions within the hypothalamus were evaluated using a combined immunohistochemical-retrograde fluorescent tracing procedure. Injections of Fluoro-gold into the anterior hypothalamus labelled TM neurons identified by their immunoreactivity for the enzyme adenosine deaminase (ADA). Small injections of Fluoro-gold into the posterior hypothalamus also led to the labelling of TM neurons. The numbers of ADA-immunoreactive axonal varicosities were 5 times greater in the hypothalamus than in most other brain regions. The results indicate that the hypothalamus represents a major projection area of the TM.
mesh_terms: D000243:Adenosine Deaminase / Q000032:analysis; D000818:Animals; D002452:Cell Count; D005456:Fluorescent Dyes; D006651:Histocytochemistry; D007032:Hypothalamus, Anterior / Q000166:cytology*; D007034:Hypothalamus, Posterior / Q000166:cytology; D007158:Immunologic Techniques; D008032:Limbic System / Q000166:cytology; D008297:Male; D008326:Mammillary Bodies / Q000166:cytology* / Q000201:enzymology; D009434:Neural Pathways / Q000166:cytology; D051381:Rats; D011919:Rats, Inbred Strains; D013266:Stilbamidines; D013687:Telencephalon / Q000166:cytology
chemical_list: C049774:2-hydroxy-4,4'-diamidinostilbene, methanesulfonate salt; D005456:Fluorescent Dyes; D013266:Stilbamidines; D000243:Adenosine Deaminase