Artery wall derived proteoglycan-plasma lipoprotein interaction: lipoprotein binding properties of extracted proteoglycans.
Artery proteoglycan-lipoprotein binding characteristics were determined using intact, high molecular weight chondroitin sulfate proteoglycans (CS-PG) isolated from grossly appearing normal aortas of atherosclerosis susceptible WC-2 pigeons and plasma lipoproteins from normolipemic, randomly bred White Carneau pigeons. Optimum formation of particulate proteoglycan-lipoprotein complexes occurred in 5 mM Tris, 6 mM KCl, 4 mM CaCl2, 1 mM MgSO4, pH 7.2. The binding of CS-PG was specific for low density lipoprotein (LDL) and not high density lipoprotein (HDL). The relative importance of the intact monomeric structure of the PG was suggested in studies where glycosaminoglycan chains isolated from the PG monomer possessed less than 1% of the binding reactivity of the intact PG. The core protein prepared from the CS-PG monomer formed no measurable particulate complex.
D000818:Animals; D001158:Arteries / Q000032:analysis*; D001161:Arteriosclerosis / Q000378:metabolism / Q000503:physiopathology; D002809:Chondroitin Sulfates / Q000302:isolation & purification / Q000378:metabolism; D010856:Columbidae; D004305:Dose-Response Relationship, Drug; D004586:Electrophoresis; D005260:Female; D006863:Hydrogen-Ion Concentration; D007477:Ions; D008074:Lipoproteins / Q000378:metabolism; D008077:Lipoproteins, LDL / Q000097:blood*; D008297:Male; D011485:Protein Binding; D011487:Protein Conformation; D011509:Proteoglycans / Q000302:isolation & purification* / Q000378:metabolism; D013696:Temperature
D007477:Ions; D008074:Lipoproteins; D008077:Lipoproteins, LDL; D011509:Proteoglycans; D002809:Chondroitin Sulfates