title: Uptake and metabolism of nucleosides by embryos of the sea urchin Strongylocentrotus purpuratus.
abstract: Uptake and metabolism of thymidine and adenosine have been studied in embryos of the sea urchin Strongylocentrotus purpuratus. Uptake of these nucleosides is found to be mutually competitive, with the Km for uptake of thymidine similar to its Ki for inhibition of adenosine uptake and vice versa. The metabolic studies show that adenosine is rapidly and completely phosphorylated upon entry, even at high exogenous concentrations which saturate the uptake mechanism. In contrast, at concentrations which saturate nucleoside uptake, thymidine becomes appreciably catabolized (up to 60%) to thymine and beta-amino-isobutyric acid in addition to its phosphorylation to thymine nucleotides. Negligible amounts of endogenous thymidine appear to remain unmetabolized following uptake in these embryos. The data provide strong in vivo evidence for separate metabolic pathways for thymidine and adenosine which have not previously been described in this organism. The observation of mutual competition during uptake, together with different routes of metabolism for these nucleosides, would suggest that the rate-limiting step in the uptake process is transport rather than metabolism. The specificity of this transport system for its nucleoside substrate has been examined in some detail in the present report. All naturally occurring nucleosides but only a limited number of nucleoside analogs are recognized by this membrane carrier. Neither purine nor pyrimidine bases are substrates for this transport system. Previous work by this laboratory has demonstrated the strict Na+-dependence of this carrier, its high affinity for nucleoside substrate, and its activation at fertilization. These observations and the substrate specificity studies of the present work together describe a unique transport system for nucleosides in sea urchin embryos which is quite different from those previously described in mammalian cells.
mesh_terms: D000241:Adenosine / Q000378:metabolism*; D000621:Aminoisobutyric Acids / Q000378:metabolism; D000818:Animals; D001667:Binding, Competitive; D001692:Biological Transport; D005976:Glutaral / Q000494:pharmacology; D007700:Kinetics; D010766:Phosphorylation; D012617:Sea Urchins / Q000187:drug effects / Q000196:embryology*; D012964:Sodium / Q000494:pharmacology; D013936:Thymidine / Q000378:metabolism*
chemical_list: D000621:Aminoisobutyric Acids; D012964:Sodium; D000241:Adenosine; D005976:Glutaral; C033435:3-aminoisobutyric acid; D013936:Thymidine