Reannealing of phalloidin-treated actin filaments during recovery after sonication and its inhibition by beta-actinin.
Phalloidin (2 mol per mol actin)-treated pyrenyl F-actin showed a critical concentration of 1.8 microM in the presence of 10 mM KCl, 0.2 mM ADP, and 5 mM Tris-HCl buffer, pH 8.0 at 25 degrees C. The filament weight concentration did not change at all during and after sonication, yet degrees of flow birefringence increased and the filament number concentration decreased after the termination of sonication. The latter changes were not affected by EDTA, but inhibited by beta-actinin. These observations suggest that reannealing of short pieces of phalloidin-treated actin filaments fragmented during sonication takes place during recovery after sonication.
D000185:Actinin / Q000494:pharmacology*; D000199:Actins; D000244:Adenosine Diphosphate / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D004492:Edetic Acid / Q000494:pharmacology; D046911:Macromolecular Substances; D009842:Oligopeptides / Q000494:pharmacology*; D010590:Phalloidine / Q000494:pharmacology*; D011817:Rabbits; D013010:Sonication; D013050:Spectrometry, Fluorescence; D014465:Ultrasonics
D000199:Actins; D046911:Macromolecular Substances; D009842:Oligopeptides; D000185:Actinin; D010590:Phalloidine; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D004492:Edetic Acid