Comparison of different sandwich enzyme immunoassays for the quantitation of human apolipoproteins A-I and A-II.
Variants of a non-competitive enzyme immunoassay for human apolipoproteins A-I and A-II are described. The method developed is highly sensitive and highly specific. There is a good correlation between the reference technique (electroimmunoassay) and both apolipoprotein A-I and A-II assays (r=0.92). For the apolipoprotein A-I assay, seven simple treatments were studied. Two of these treatments (plasma plus Tween 20 and serum plus apo A-II) greatly increased the precision and the accuracy of the results. For the apo A-II measurement, no treatment of the samples is necessary, but plasma should be preferentially used rather than serum.
D016632:Apolipoprotein A-I; D016633:Apolipoprotein A-II; D001054:Apolipoproteins A / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D007124:Immunoenzyme Techniques; D011136:Polysorbates
D016632:Apolipoprotein A-I; D016633:Apolipoprotein A-II; D001054:Apolipoproteins A; D011136:Polysorbates