Primary structure of soybean lipoxygenase-1.
The primary structure of lipoxygenase-1 from soybeans has been determined. The results were deduced from the nucleotide sequence of the complete cDNA for isozyme lipoxygenase-1 and were confirmed by Edman sequencing of selected peptides obtained by CNBr cleavage and supported by carboxyl-terminal sequencing of the intact protein and by partial peptide analysis employing fast atom bombardment-mass spectroscopy. The enzyme has a molecular weight of 94,038 based on its content of 838 amino acids.
D000595:Amino Acid Sequence; D003488:Cyanogen Bromide; D004247:DNA / Q000032:analysis; D007527:Isoenzymes / Q000032:analysis / Q000235:genetics; D008084:Lipoxygenase / Q000032:analysis* / Q000235:genetics; D013058:Mass Spectrometry; D008970:Molecular Weight; D013025:Glycine max / Q000201:enzymology
D007527:Isoenzymes; D004247:DNA; D008084:Lipoxygenase; D003488:Cyanogen Bromide