Killing of schistosomula of Schistosoma mansoni by macrophages: induction by T-cell clone-derived lymphokines and interferon-gamma.
The induction of schistosomulicidal activity of peritoneal macrophages by concanavalin A-stimulated supernatants from long-term T-cell clones and by interferon-gamma (IFN-gamma) was investigated in detail. Optimal conditions of in vitro macrophage activation by T-cell clone supernatants were established. Macrophages from 13-week S. mansoni-infected mice responded to lymphokine activation as well as resident macrophages from uninfected mice. IFN-gamma was shown to play an essential role in induction of schistosomulicidal macrophage activity: recombinant IFN-gamma at high concentration could induce schistosomula killing, and an anti-IFN-gamma antiserum inhibited the induction of schistosomulicidal activity by T-cell clone supernatants. Our data also indicate that macrophage activation could be obtained by IFN-gamma in synergy with other lymphokines in the supernatant of long-term T-cell clones. Macrophages from mice injected with T-cell clone supernatants were primed in vivo and triggered to kill schistosomula in vitro in the presence of lipopolysaccharide (LPS). The data demonstrate that lymphokines produced by T-cell clones and, in particular, IFN-gamma can participate in the activation of schistosomulicidal macrophages.
D000818:Animals; D002999:Clone Cells; D003208:Concanavalin A / Q000494:pharmacology; D007371:Interferon-gamma / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008213:Lymphocyte Activation; D008222:Lymphokines / Q000494:pharmacology*; D008262:Macrophage Activation; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D008809:Mice, Inbred C3H; D008811:Mice, Inbred DBA; D012550:Schistosoma mansoni / Q000276:immunology*; D012555:Schistosomiasis mansoni / Q000469:parasitology; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism
D008070:Lipopolysaccharides; D008222:Lymphokines; D003208:Concanavalin A; D007371:Interferon-gamma