Pituitary responsiveness of mares challenged with GnRH at various stages of the transition into the breeding season.
Four groups of mares, representing anestrus (AN; n = 8), early transition (ET; n = 7), late transition (LT; n = 8) and estrus (EST; n = 12) were used to examine release of luteinizing hormone (LH) and follicle stimulating hormone (FSH) after a bolus injection of gonadotropin releasing hormone (GnRH) during the transition from anestrus into the breeding season. Estrous mares received GnRH on d 2 or 3 of estrus in the cycle immediately preceding slaughter. Anestrous, ET and LT mares received GnRH exactly 1 wk prior to slaughter. A single injection of GnRH (Sigma LHRH, L-0507, 2.0 micrograms/kg body weight in .9% saline, iv) was given to each mare. Blood samples were collected at -2, h, -1 h, directly prior to GnRH, then 15, 30, 45, 60, 90, 120, 150, 180, 210, 240, 300, 360, 420 and 480 min post-injection. Maximum release of LH and FSH was observed within 30 min after injection of GnRH. Except for the LH response in EST mares, concentrations of both hormones had returned to pre-injection baseline levels within 8 h. Group means for area under the curve (AUC) of concentrations of LH in serum, and the maximum amount (MAX) of LH quantified in serum, post-GnRH, increased (P less than .05) progressively from AN to the breeding season. The AUC and MAX responses for FSH showed a reverse pattern, decreasing (P less than .05) from AN to the breeding season.(ABSTRACT TRUNCATED AT 250 WORDS)
D000780:Anestrus / Q000187:drug effects; D000818:Animals; D004971:Estrus / Q000187:drug effects*; D005260:Female; D005640:Follicle Stimulating Hormone / Q000378:metabolism*; D006736:Horses / Q000502:physiology*; D007986:Luteinizing Hormone / Q000378:metabolism*; D010906:Pituitary Hormone-Releasing Hormones / Q000494:pharmacology*
D010906:Pituitary Hormone-Releasing Hormones; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone