Inactivation of human T-cell lymphotropic virus, type III by heat, chemicals, and irradiation.
Infectivity of human T-cell lymphotropic virus, Type III (HTLV-III) was inactivated by heat more rapidly if in liquid medium than if lyophilized and more rapidly at 60 degrees than 56 degrees C. When HTLV-III was added to factor VIII suspension, then lyophilized and heated at 60 degrees C for 2 hours or longer there was elimination of 1 X 10(6) in vitro infectious units (IVIU) of virus. Much of the viral inactivation appeared to result from lyophilization. The application of water-saturated chloroform to the lyophilized material containing virus also resulted in elimination of infectivity. HTLV-III was efficiently inactivated by formalin, beta-propiolactone, ethyl ether, detergent, and ultraviolet light plus psoralen. The results are reassuring regarding the potential safety of various biological products.
D002725:Chloroform; D003470:Culture Media; D005169:Factor VIII; D006678:HIV / Q000254:growth & development*; D006358:Hot Temperature; D006801:Humans; D008722:Methods; D014775:Virus Activation / Q000187:drug effects / Q000528:radiation effects
D003470:Culture Media; D002725:Chloroform; D005169:Factor VIII