title: Reactivity of a monoclonal antibody recognizing an estrogen receptor regulated glycoprotein in relation to lectin histochemistry in breast cancer.
abstract: We have raised monoclonal antibodies against human milk fat globule membrane antigens and previously shown that one of them, called III D 5, recognises a glycoprotein associated with estrogen receptor activity of breast cancer. In immunoblotting it was shown that the molecule in human milk exclusively stained with III D 5 also binds peanut agglutinin (PNA) and Ricinus communis. In this study we correlate the staining of III D 5 and binding of lectins to tissue sections fixed in formalin and embedded in paraffin. Similar reactions were seen only with III D 5 and PNA. Our results suggest that III D 5 and PNA detect overlapping antigenic epitopes in mammary carcinoma. This is in keeping with previous results that PNA or III D 5 reactivity is correlated with estrogen receptor status of breast cancer.
mesh_terms: D000911:Antibodies, Monoclonal; D001943:Breast Neoplasms / Q000032:analysis*; D003429:Cross Reactions; D006651:Histocytochemistry; D006801:Humans; D037102:Lectins / Q000032:analysis*; D008565:Membrane Proteins / Q000276:immunology; D008895:Milk, Human / Q000032:analysis; D009363:Neoplasm Proteins / Q000032:analysis* / Q000276:immunology; D019887:Peanut Agglutinin; D011975:Receptors, Mitogen / Q000032:analysis; D012276:Ricin / Q000032:analysis; D014909:Wheat Germ Agglutinins / Q000032:analysis
chemical_list: D000911:Antibodies, Monoclonal; D037102:Lectins; D008565:Membrane Proteins; D009363:Neoplasm Proteins; D019887:Peanut Agglutinin; D011975:Receptors, Mitogen; D014909:Wheat Germ Agglutinins; C038865:estrogen regulated protein; D012276:Ricin