title: Ir gene control of the murine secretory IgA response to cholera toxin.
abstract: In these experiments we examined the genetic control of the secretory IgA (sIgA) response to cholera toxin (CT) after CT feeding. Inbred, congenic and intra-H-2I region recombinant mouse strains were immunized with intragastric application of 10 micrograms CT on days 0 and 14. Samples of intestinal secretions and plasma were collected 1 week after the second dose and antibodies to CT measured in them by antigen- and isotype-specific enzyme-linked immunosorbent assay. In three different sets of H-2-congenic strains the intestinal IgA anti-CT response clearly depended on the H-2 haplotype rather than on background or IgH genes. H-2b (B10, A.BY/SnJ, C3H.SW) and H-2q (B10.T(6R), DBA/1J) strains were high responders, H-2k (B10.BR, C3H/He), H-2s (A.SW/SnJ) and H-2d (B10.D2) strains were low responders. Within the H-2 complex the intestinal IgA anti-CT response was mapped to the I-A subregion with the use of congenic intra-H-2I region recombinant strains: B10.A(3R) and B10.A(5R) were high responders and B10.A(4R), B10.MBR and B10.GD were low responders. Plasma IgG anti-CT after CT feeding paralleled the sIgA results. Surprisingly, the sIgA and plasma IgG anti-CT responses in individual mice of the various strains tested showed a highly significant positive correlation. We conclude that both the sIgA response and plasma IgG anti-CT response after CT feeding is controlled by the I-A subregion of H-2.
mesh_terms: D000818:Animals; D000992:Antitoxins / Q000235:genetics / Q000276:immunology; D002772:Cholera Toxin / Q000276:immunology*; D005802:Genes, MHC Class II; D007071:Immunoglobulin A, Secretory / Q000235:genetics* / Q000276:immunology; D007074:Immunoglobulin G / Q000235:genetics / Q000276:immunology; D007156:Immunologic Memory; D007422:Intestines / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains
chemical_list: D000992:Antitoxins; D007071:Immunoglobulin A, Secretory; D007074:Immunoglobulin G; D002772:Cholera Toxin