Apparent in vivo retroconversion of dietary arachidonic to linoleic acid in essential fatty acid-deficient rats.
Essential fatty acid-deficient rats were fed ethyl [U-14C]arachidonate (308 dpm/nmol) and when a decrease in the transepidermal water loss was seen, the epidermal sphingolipids, acylglucosylceramide and acylceramide were isolated. [14C]Linoleic acid (approx. 130 dpm/nmol) was present in both lipid classes, while the substrate was only detected in the former. These results intimate that in vivo retroconversion of arachidonic to linoleic acid can be induced in the rat.
D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism*; D002250:Carbon Radioisotopes; D005228:Fatty Acids, Essential / Q000172:deficiency*; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000378:metabolism*; D051381:Rats; D012867:Skin / Q000378:metabolism; D013107:Sphingolipids / Q000378:metabolism
D001095:Arachidonic Acids; D002250:Carbon Radioisotopes; D005228:Fatty Acids, Essential; D008041:Linoleic Acids; D013107:Sphingolipids; D016718:Arachidonic Acid; D019787:Linoleic Acid