Determination and characterization of succinyl tri-alanine p-nitroanilide hydrolyzing metalloendopeptidase in serum.
Serum succinyl (Ala)3-p-nitroanilide hydrolyzing elastase-like activity which elevates in patients with obstructive jaundice, is due to the joint action of two enzymes: first, succinyl (Ala)3-p-nitroanilide is cleaved to succinyl (Ala)2 and Ala-p-nitroanilide by metalloendopeptidase, and then Ala-p-nitroanilide is cleaved to Ala and p-nitroaniline by aminopeptidase. We adopt a new assay method for serum endopeptidase activity using HPLC.
D000328:Adult; D000626:Aminopeptidases / Q000037:antagonists & inhibitors / Q000097:blood*; D000900:Anti-Bacterial Agents; D002779:Cholestasis / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003920:Diabetes Mellitus / Q000201:enzymology; D010450:Endopeptidases / Q000097:blood*; D005260:Female; D006020:Glycopeptides / Q000494:pharmacology; D006801:Humans; D005779:Immunodiffusion; D007668:Kidney / Q000201:enzymology; D007930:Leucine / Q000031:analogs & derivatives / Q000494:pharmacology; D008297:Male; D008666:Metalloendopeptidases; D009369:Neoplasms / Q000201:enzymology; D009842:Oligopeptides / Q000378:metabolism* / Q000494:pharmacology; D010455:Peptides; D011480:Protease Inhibitors
D000900:Anti-Bacterial Agents; D006020:Glycopeptides; D009842:Oligopeptides; D010455:Peptides; D011480:Protease Inhibitors; C015516:succinyl-trialanine-4-nitroanilide; C016977:amastatin; D010450:Endopeptidases; D000626:Aminopeptidases; D008666:Metalloendopeptidases; D007930:Leucine; C012211:ubenimex; C008890:phosphoramidon