[Kinetic study of 3,4-dihydroxyphenyl-L-alanine synthesis in Citrobacter freundii cells immobilized in carrageenan].
A kinetic study was carried out of the enzymatic synthesis of 3,4-dihydroxyphenyl-L-alanine (DOPA) by the Citrobacter freundii 62 cells, possessing tyrosine-phenol-lyase (TPL) activity, immobilized in carrageenan, and optimum conditions of the reaction were found. The dependence of the TPL activity and its stability on the conditions of the DOPA synthesis was investigated. The TPL activity was higher and more stable in the immobilized cells as compared to free ones.
D001431:Bacteriological Techniques; D002351:Carrageenan; D002954:Citrobacter / Q000378:metabolism*; D004295:Dihydroxyphenylalanine / Q000096:biosynthesis*; D007700:Kinetics
D004295:Dihydroxyphenylalanine; D002351:Carrageenan
Kineticheskoe izuchenie sinteza 3,4-dioksifenil-L-alanina kletkami Citrobacter freundii, immobilizovannymi v karraginan.