title: Transduction proteins of olfactory receptor cells: identification of guanine nucleotide binding proteins and protein kinase C.
abstract: We have analyzed guanine nucleotide binding proteins (G-proteins) in the olfactory epithelium of Rana catesbeiana using subunit-specific antisera. The olfactory epithelium contained the alpha subunits of three G-proteins, migrating on polyacrylamide gels in SDS with apparent molecular weights of 45,000, 42,000, and 40,000, corresponding to Gs, Gi, and Go, respectively. A single beta subunit with an apparent molecular weight of 36,000 was detected. An antiserum against the alpha subunit of retinal transducin failed to detect immunoreactive proteins in olfactory cilia detached from the epithelium. The olfactory cilia appeared to be enriched in immunoreactive Gs alpha relative to Gi alpha and Go alpha when compared to membranes prepared from the olfactory epithelium after detachment of the cilia. alpha subunits of G-proteins were not detected in cilia detached from the nonchemosensory respiratory epithelium of the palate. Immunohistochemical studies using an antiserum against the beta subunit of G-proteins revealed intense staining of the ciliary surface of the olfactory epithelium and of the axon bundles in the lamina propria. In contrast, an antiserum against a common sequence of the alpha subunits preferentially stained the cell membranes of the olfactory receptor cells and the acinar cells of Bowman's glands and the deep submucosal glands. Prolonged incubation periods with these antisera tended to obliterate these differences in staining patterns, giving rise to staining by both antisera of the ciliary surface, the olfactory receptor cell membranes, the axon bundles, and the acinar cells of the glands. In addition to G-proteins, we have identified protein kinase C in olfactory cilia via a protein kinase C specific antiserum and via phorbol ester binding.(ABSTRACT TRUNCATED AT 250 WORDS)
mesh_terms: D000818:Animals; D029742:Caenorhabditis elegans Proteins; D002352:Carrier Proteins; D002462:Cell Membrane / Q000378:metabolism; D002923:Cilia / Q000378:metabolism; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000302:isolation & purification / Q000378:metabolism*; D007106:Immune Sera; D008465:Mechanoreceptors / Q000378:metabolism*; D008970:Molecular Weight; D009297:Nasal Mucosa / Q000166:cytology / Q000378:metabolism*; D015240:Phorbol 12,13-Dibutyrate; D010703:Phorbol Esters / Q000378:metabolism; D011493:Protein Kinase C / Q000302:isolation & purification / Q000378:metabolism*; D011892:Rana catesbeiana; D011955:Receptors, Drug; D011971:Receptors, Immunologic / Q000378:metabolism; D012903:Smell
chemical_list: D029742:Caenorhabditis elegans Proteins; D002352:Carrier Proteins; D007106:Immune Sera; D010703:Phorbol Esters; D011955:Receptors, Drug; D011971:Receptors, Immunologic; C083141:phorbol ester binding protein; C027776:phorbol ester receptor; D015240:Phorbol 12,13-Dibutyrate; D011493:Protein Kinase C; D019204:GTP-Binding Proteins