Detection of minimal residual cells carrying the t(14;18) by DNA sequence amplification.
By means of the polymerase chain reaction (PCR) technique, DNA sequences were amplified that flank the crossover sites of a characteristic chromosomal translocation for follicular lymphomas, t(14;18)(q32;q21). This technique permitted the detection of cells carrying the t(14;18) hybrid DNA sequences at a dilution of 1:100,000. The remission marrow and blood samples of a patient with follicular lymphoma and the t(14;18) failed to show any abnormality by morphological examination and conventional Southern blot analysis. However, the t(14;18) hybrid DNA sequences were detected by the PCR technique. Thus, this technique is a highly sensitive tool to detect minimal residual cells carrying the t(14;18) and has the potential to identify a subpopulation of patients with subclinical disease.
D001483:Base Sequence; D002883:Chromosomes, Human, Pair 14 / Q000648:ultrastructure*; D002887:Chromosomes, Human, Pair 18 / Q000648:ultrastructure*; D004273:DNA, Neoplasm / Q000032:analysis*; D005784:Gene Amplification; D005819:Genetic Markers; D006801:Humans; D007144:Immunoglobulin J-Chains / Q000235:genetics; D008199:Lymphadenitis / Q000235:genetics; D008224:Lymphoma, Follicular / Q000235:genetics*; D009364:Neoplasm Recurrence, Local / Q000175:diagnosis; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000235:genetics; D014178:Translocation, Genetic
D004273:DNA, Neoplasm; D005819:Genetic Markers; D007144:Immunoglobulin J-Chains; D009838:Oligodeoxyribonucleotides