Cultivation of Bowes melanoma cells in the Opticell system: influence of the addition of protein supplements to the serum-free medium on the production of plasminogen activator.
The influence of a protein-free and a protein-rich, supplemented serum-free medium on the production of plasminogen activator (t-PA) from Bowes melanoma cells was investigated in the Opticell culture system and compared to tissue culture flask cultures. In the presence of medium supplements metabolic activity and t-PA production were favoured in both systems. The addition of supplements was apparently more effective in the Opticell than in flask cultures, because t-PA activity obtained in the Opticell was 2-3 times higher in protein-rich medium, but 2 times lower in unsupplemented medium than in flasks. These results indicate that the protein content in a serum-free medium is important for product formation in the Opticell, and serum-free media which work at small scale in tissue culture flasks are not always suited for technical culture systems such as the Opticell but have to be adapted to them.
D001709:Biotechnology / Q000295:instrumentation; D001769:Blood; D002448:Cell Adhesion; D002460:Cell Line; D002478:Cells, Cultured; D003470:Culture Media; D005947:Glucose / Q000378:metabolism; D005973:Glutamine / Q000378:metabolism; D006801:Humans; D008546:Melanoma, Experimental / Q000473:pathology*; D012694:Serine / Q000378:metabolism; D010959:Tissue Plasminogen Activator / Q000096:biosynthesis*
D003470:Culture Media; D005973:Glutamine; D012694:Serine; D010959:Tissue Plasminogen Activator; D005947:Glucose