Solubilization and partial purification of aromatase from chicken ovary.
Aromatase, the cytochrome P-450 that converts androgen to estrogen, has been solubilized from chicken ovarian microsomes with the nonionic detergent Emulgen 913. Following chromatography on gel filtration, anion exchange, dye affinity, and hydrophobic media, ovarian aromatase is purified up to 27-fold with 10-15% recovery. Separation of the cytochrome P-450 aromatase from NADPH cytochrome P-450 reductase is achieved during the purification. The partially purified enzyme is stable for as long as 6 months when frozen in liquid nitrogen in buffer containing dithiothreitol, glycerol, Emulgen and 150 mM KCl.
D000818:Animals; D001141:Aromatase / Q000032:analysis / Q000302:isolation & purification*; D002645:Chickens; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D005260:Female; D009251:NADPH-Ferrihemoprotein Reductase / Q000032:analysis; D010053:Ovary / Q000201:enzymology*; D012995:Solubility
D001141:Aromatase; D009251:NADPH-Ferrihemoprotein Reductase