AKR murine thymic leukemias are from a distinct thymic cell lineage and do not express the beta chain of the T-cell antigen receptor.
Characterization of tumors that arise spontaneously in the AKR mouse indicates that they are derived from cells of a distinct T-cell lineage. Cells in this subclass bear surface antigens, designated Tpre, Tthy, Tind, and Tsu, which are encoded by genes in the Tsu linkage group on murine chromosome 12. We have examined the rearrangement and expression of genes encoding the T-cell alpha, beta, and gamma chains in these tumors. Although these cells contain alpha-chain mRNA, they do not produce a normal-sized beta-chain mRNA. Most of them also lack gamma-chain mRNA. Each thymic leukemia was derived from a cell arrested at a different stage of development as defined by their expression of terminal deoxynucleotidyl transferase and Thy-1 mRNA. The data presented here are consistent with a model in which thymocytes expressing Tpre, Tthy, Tind, or Tsu undergo somatic development parallel to the development of other T cells. However, these thymocytes do not appear to differentiate into cells bearing alpha-beta heterodimers of the T-cell antigen receptor.
D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface / Q000235:genetics; D002454:Cell Differentiation; D004253:DNA Nucleotidylexotransferase / Q000235:genetics; D005786:Gene Expression Regulation; D005796:Genes; D007942:Leukemia, Experimental / Q000235:genetics / Q000276:immunology / Q000473:pathology*; D046911:Macromolecular Substances; D051379:Mice; D008806:Mice, Inbred AKR; D012333:RNA, Messenger / Q000235:genetics; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*; D011995:Recombination, Genetic; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000502:physiology
D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface; D046911:Macromolecular Substances; D012333:RNA, Messenger; D011948:Receptors, Antigen, T-Cell; D004253:DNA Nucleotidylexotransferase