[Induction of the switch in the synthesis of immunoglobulin classes in the RPM 1-6410t lymphoblast cell line and its clones as affected by fetal calf serum factors].
We have found that human lymphoblastoid cell line RPMI-6410t is a biochemical mutant for gene of thymidine kinase and has chromosome markers in the karyotype. Thus, this cell line can be used as a partner in somatic hybridization, in particular for producing hybridomas, synthesizing human monoclonal antibodies. We have discovered that line RPMI-6410t carries HLA-A2, -B7 and -B12 antigens of human histocompatibility complex on the cell surface. The cell membrane of this cell line contains immunoglobulins of M and D classes. RPMI-6410t cells secrete IgM molecules. It is demonstrated that induction of the switch of immunoglobulin heavy-chain classes by the factors of foetal calf serum takes place in the cells of RPMI-6410t line. Thus, the corresponding stage of B-lymphocytes differentiation in vivo is reproduced in 6410t line in vitro.
D000818:Animals; D002417:Cattle; D002454:Cell Differentiation; D002460:Cell Line; D002999:Clone Cells / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology*; D007143:Immunoglobulin Heavy Chains / Q000032:analysis / Q000096:biosynthesis; D007136:Immunoglobulins / Q000032:analysis / Q000096:biosynthesis*; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D011947:Receptors, Antigen, B-Cell / Q000032:analysis / Q000096:biosynthesis
D007106:Immune Sera; D007143:Immunoglobulin Heavy Chains; D007136:Immunoglobulins; D011947:Receptors, Antigen, B-Cell
Induktsiia perekliucheniia sinteza klassov immunoglobulinov v linii limfoblastoidnykh kletok RPM 1-6410t i ee klonakh pod deĭstviem faktorov émbrional'noĭ teliach'eĭ syvorotki.