Competition between tetracycline and tRNA at both P and A sites of the ribosome of Escherichia coli.
Fluorescence anisotropy studies performed on 6-demethylchlortetracycline, binding to the ribosome of E. coli in competition with tRNA at the P site or at both P and A sites, have provided a quantitative assessment in situ of the interaction of this antibiotic with the A site and have demonstrated that there is also an interaction between tetracycline and the P site.
D001665:Binding Sites; D001667:Binding, Competitive; D003707:Demeclocycline / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism*; D005454:Fluorescence Polarization; D012343:RNA, Transfer / Q000378:metabolism*; D012346:RNA, Transfer, Amino Acyl / Q000378:metabolism; D012358:RNA, Transfer, Met; D012270:Ribosomes / Q000378:metabolism*
D012346:RNA, Transfer, Amino Acyl; D012358:RNA, Transfer, Met; C024988:tRNA, formylmethionine-; C037801:tRNA, phenylalanylphenylalanine-; D003707:Demeclocycline; D012343:RNA, Transfer