Lectin coated colloidal gold as a marker for mesoderm formation in mammalian embryos, cultured in vitro.
A simple in vitro culture system for mammalian embryos of pre-somite to 30-somite stages is described, in which mouse and rat embryos are used. The rat embryo culture turned out to be more successful for periods longer than 8 hr. The formation of mesoderm and mesectoderm was studied in these embryos by labeling the primitive streak and neural crest with lectin-coated colloidal gold, that was injected into the amniotic cavity with a micro-injection system.
D000818:Animals; D046508:Culture Techniques / Q000379:methods; D006046:Gold / Q000378:metabolism; D006257:Head / Q000196:embryology; D037102:Lectins; D008648:Mesoderm / Q000502:physiology* / Q000648:ultrastructure; D051379:Mice; D008815:Mice, Inbred Strains; D008855:Microscopy, Electron, Scanning; D010873:Pinocytosis; D051381:Rats; D011919:Rats, Inbred Strains
D037102:Lectins; D006046:Gold