Purification of antibody against peanut agglutinin-receptors of bovine interphotoreceptor matrix.
From rabbit antiserum against peanut agglutinin (PNA)-receptors of the bovine interphotoreceptor matrix, an antibody was isolated by immunoaffinity chromatography on immobilized PNA-receptors. The antibody reacted with the bovine interphotoreceptor matrix at the molecular weight of 130,000 daltons. Immunohistochemical studies revealed that the antibody was bound not only to the surfaces of the cone photoreceptor cells but also to the surfaces of the rods, suggesting that the antibody purified here may have been formed by the major structure of glycoprotein that is common in the interphotoreceptor matrix around the cones and rods.
D000818:Animals; D000906:Antibodies / Q000276:immunology / Q000302:isolation & purification*; D001666:Binding Sites, Antibody; D002417:Cattle; D006023:Glycoproteins / Q000276:immunology / Q000378:metabolism; D000882:Haplorhini; D006651:Histocytochemistry; D007120:Immunochemistry; D010786:Photoreceptor Cells / Q000276:immunology / Q000378:metabolism*; D011975:Receptors, Mitogen / Q000276:immunology*; D012160:Retina / Q000276:immunology
D000906:Antibodies; D006023:Glycoproteins; D011975:Receptors, Mitogen; C038223:peanut agglutinin receptor