Isolation and cross-sensitivity of X-ray-sensitive mutants of V79-4 hamster cells.
The V79-4 Chinese hamster line was mutagenized and surviving clones screened for X-ray sensitivity using a replica microwell technique. One slightly sensitive clone and 3 clearly sensitive clones were isolated from approximately 5000 screened, and designated irs 1 to irs 4. The 3 more sensitive clones showed different responses to the genotoxic agents mitomycin C (MMC), ethyl methanesulphonate (EMS) and ultraviolet light (UV). irs 1 showed considerable sensitivity to all the agents tested, in the order MMC much greater than EMS greater than UV. irs 2 and irs 3 had similar sensitivities to EMS and to UV (EMS greater than UV) but irs 3 was more sensitive than irs 2 to MMC. None of these mutants is identical in phenotype to previously published mutants.
D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000528:radiation effects; D002478:Cells, Cultured / Q000528:radiation effects; D006224:Cricetinae; D003412:Cricetulus; D004260:DNA Repair; D005020:Ethyl Methanesulfonate / Q000494:pharmacology; D005038:Ethylnitrosourea; D007621:Karyotyping; D016685:Mitomycin; D008937:Mitomycins / Q000494:pharmacology; D009154:Mutation; D014466:Ultraviolet Rays; D014965:X-Rays
D008937:Mitomycins; D016685:Mitomycin; D005020:Ethyl Methanesulfonate; D005038:Ethylnitrosourea