Lapachol inhibition of DT-diaphorase (NAD(P)H:quinone dehydrogenase).
Lapachol has been found to be a potent inhibitor of the enzyme DT-Diaphorase. Inhibition is competitive versus NADH, Ki = 0.15 microM. Lapachol was not a good substrate for cytochrome P450 reductase, thus inhibition of DT-Diaphorase should not promote its metabolism via radical generating pathways. DT-Diaphorase has been used to test a lapachol affinity chromatography column designed for purification of another coumarin anticoagulant and lapachol sensitive enzyme, vitamin K epoxide reductase.
D000818:Animals; D002846:Chromatography, Affinity; D003600:Cytosol / Q000201:enzymology; D066298:In Vitro Techniques; D008862:Microsomes, Liver / Q000201:enzymology; D009243:NAD / Q000378:metabolism; D016660:NAD(P)H Dehydrogenase (Quinone); D009285:Naphthoquinones / Q000378:metabolism / Q000494:pharmacology*; D010084:Oxidation-Reduction; D011808:Quinone Reductases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D051381:Rats
D009285:Naphthoquinones; D009243:NAD; C008252:lapachol; D016660:NAD(P)H Dehydrogenase (Quinone); D011808:Quinone Reductases