A hemagglutination test for binding of hydrazide-derivatized cytochemical markers.
A sensitive hemagglutination test was developed in order to predict the ability of amine- or hydrazide-derivatized cytochemical markers to interact with cell surface aldehydes in various in vitro and in situ systems. Human red blood cells were modified by glutaraldehyde fixation and subsequent oxidation with sodium periodate, thus exposing free aldehyde residues on their surface, able to form Schiff bases with accessible hydrazide groups on the marker molecule. Fixed and oxidized erythrocytes could be stored for more than 1 year without losing their capacity to be agglutinated by polyhydrazides. The aggregating capacity of hydrazide-enriched molecules correlated well with their binding to oxidized cell surface moieties.
D000447:Aldehydes / Q000032:analysis; D000588:Amines; D004910:Erythrocyte Membrane / Q000276:immunology / Q000648:ultrastructure*; D006386:Hemagglutination Tests; D006801:Humans; D006834:Hydrazines; D007202:Indicators and Reagents; D008854:Microscopy, Electron
D000447:Aldehydes; D000588:Amines; D006834:Hydrazines; D007202:Indicators and Reagents