title: Diminished steroidogenic response of hamster granulosa cells to estrogen in vitro.
abstract: We have previously demonstrated that estrogen can exert inhibitory or atretogenic effects on the ovaries of both rats and rhesus monkeys in vivo. This study was designed to test whether the hamster (Mesocricetus auratus) is an appropriate model in which to test the effects of estrogens (diethylstilbestrol and estradiol-17 beta) on steroid accumulation by ovarian granulosa cells in vitro, and whether the effects are similar to those demonstrated for other species in vivo. Immature female hamsters were injected with pregnant mare's serum gonadotropin at 28 to 30 days of age. Animals were sacrificed and follicular contents aspirated three days later. Granulosa cells were either left untreated or treated with diethylstilbestrol or estradiol (1 X 10(-7) M) in vitro for 72 h in the presence of androstenedione (1 X 10(-7) M, and in the presence or absence of serum (10%) or human follicle-stimulating hormone (20 ng/ml), and long-term accumulation of estrogen and progesterone was determined. Diethylstilbestrol inhibited accumulation of estrogen regardless of the presence or absence of follicle-stimulating hormone. In contrast, only estradiol plus follicle-stimulating hormone augmented accumulation of progesterone by granulosa cells. These findings that estrogen can be non-stimulatory or inhibitory to function of granulosa cells in vitro parallel those shown in vivo. Our experimental approach may therefore represent an appropriate model for study of the direct effects of estradiol on the function of granulosa cells.
mesh_terms: D000735:Androstenedione / Q000494:pharmacology; D000818:Animals; D002478:Cells, Cultured; D006224:Cricetinae; D004054:Diethylstilbestrol / Q000494:pharmacology*; D004958:Estradiol / Q000494:pharmacology*; D004967:Estrogens / Q000096:biosynthesis*; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D006064:Gonadotropins, Equine / Q000494:pharmacology; D006107:Granulosa Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D007700:Kinetics; D008647:Mesocricetus; D006080:Ovarian Follicle / Q000502:physiology; D011374:Progesterone / Q000096:biosynthesis*
chemical_list: D004967:Estrogens; D006064:Gonadotropins, Equine; D000735:Androstenedione; D011374:Progesterone; D004958:Estradiol; D004054:Diethylstilbestrol; D005640:Follicle Stimulating Hormone