[In vitro release of hypothalamic corticoliberine in the rat: incubation of slices from the whole hypothalamus].
An experimental system allowing both the incubation and rapid transfert of rat hypothalamic slices has been developed in order to approach the regulation of CRF secretion. The release of CRF has been quantified by a specific radioimmunoassay. Under basal conditions, immunoreactive CRF release reached an optimum of 96.2 +/- 10.4 pg/3 hypothalami/20 min. A depolarizing concentration of KCl (56 mM) or veratridine (50 microM) applied for 20 min. induced a 222 and 257% increase, respectively, in CRF release. The in vitro CRF values released under basal and stimulated conditions are comparable to those of other hypothalamic neuropeptides. Furthermore, in vitro CRF release from the hypothalamus is in the same order of magnitude as in vivo CRF secretion estimated by hypophysial portal blood collection or median eminence push-pull cannulation.
D000818:Animals; D003346:Corticotropin-Releasing Hormone / Q000032:analysis / Q000378:metabolism*; D007031:Hypothalamus / Q000032:analysis / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D011189:Potassium Chloride / Q000494:pharmacology; D011526:Protoveratrines / Q000494:pharmacology; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains
D011526:Protoveratrines; D011189:Potassium Chloride; D003346:Corticotropin-Releasing Hormone
Libération in vitro de la corticoliberine (CRF) hypothalamique de rat: incubation de coupes d'hypothalamus total.