Reduced survival of isotope-labelled Rh(D)-negative donor red cells in a patient with anti-LWab.
The serum of an 85-year-old Caucasian male with no history of blood transfusion contained an IgG3 antibody with anti-LWab specificity. The antibody failed to react with dithiothreitol-treated red cells, and there was a marked reduction in titre of the antibody with pronase-treated cells, findings consistent with an antibody having this specificity. High association values were obtained in a mononuclear phagocyte assay when LW-positive red cells, sensitised in vitro with the patient's serum antibody, were incubated with peripheral blood monocytes from the patient. In vivo red cell survival studies demonstrated that 99mTc-labelled rhesus-negative (rr), LW-positive red cells had 53% survival at 1 h. The IgG subclass of the antibody, mononuclear phagocyte assay results and in vivo survival studies predicted a significant reduction in the posttransfusion survival of therapeutic volumes of rhesus-negative (rr), LW-positive red cells.
D000368:Aged; D000369:Aged, 80 and over; D001789:Blood Group Antigens; D004905:Erythrocyte Aging; D006801:Humans; D007518:Isoantibodies / Q000276:immunology*; D009000:Monocytes / Q000276:immunology; D010587:Phagocytosis; D012204:Rh-Hr Blood-Group System
D001789:Blood Group Antigens; D007518:Isoantibodies; D012204:Rh-Hr Blood-Group System