Estimates of the rate of ligation during excision repair of ultraviolet-damaged DNA in mammalian cells.
When ultraviolet-irradiated mammalian cells are incubated with inhibitors of repair DNA synthesis, incomplete repair sites--seen as DNA breaks--accumulate. If the inhibition is reversed, the breaks are joined. Thus the ligation step of excision repair can be investigated. With aphidicolin as inhibitor, ligation occurs at up to 15-times the rate of incision. 3-Aminobenzamide (which inhibits poly(ADPribose) synthesis) does not delay the rejoining of DNA breaks.
D000246:Adenosine Diphosphate Ribose / Q000378:metabolism; D000818:Animals; D016590:Aphidicolin; D001549:Benzamides / Q000494:pharmacology; D002478:Cells, Cultured; D006224:Cricetinae; D003561:Cytarabine / Q000494:pharmacology; D004247:DNA / Q000528:radiation effects*; D004249:DNA Damage; D004260:DNA Repair / Q000187:drug effects; D004224:Diterpenes / Q000494:pharmacology; D006801:Humans; D006918:Hydroxyurea / Q000494:pharmacology; D007700:Kinetics; D014466:Ultraviolet Rays
D001549:Benzamides; D004224:Diterpenes; D003561:Cytarabine; D000246:Adenosine Diphosphate Ribose; D016590:Aphidicolin; C025160:3-aminobenzamide; D004247:DNA; D006918:Hydroxyurea