The effect of arcuate nucleus transplantation on the development of the anterior pituitary in monosodium glutamate-treated rats.
Treatment with monosodium glutamate (MSG) during the neonatal period is known to produce a selective lesion of the arcuate nucleus in rat brain, which is the major site of production of growth hormone releasing-hormone (GRH), followed by a secondary reduction in growth hormone (GH) synthesis in the anterior pituitary. Normal arcuate nuclei from hypothalamic areas of newborn rats were transplanted into the third ventricles of 27-day-old rats which were treated with MSG on alternate days for the first 10 days of life. Ninety days after birth, the anterior pituitaries were examined for GH synthesis by immunohistochemical staining with GH antiserum. The results indicated that the impaired GH synthesis in the anterior pituitary treated with MSG was partially restored in some recipients by grafts of arcuate nuclei in which the GRH-containing neurons were clearly detected by immunohistochemical staining with GRH antiserum.
D000818:Animals; D000831:Animals, Newborn; D001111:Arcuate Nucleus of Hypothalamus / Q000378:metabolism / Q000637:transplantation*; D005260:Female; D013006:Growth Hormone / Q000172:deficiency*; D013007:Growth Hormone-Releasing Hormone / Q000378:metabolism; D007027:Hypothalamic Diseases / Q000139:chemically induced / Q000628:therapy; D010903:Pituitary Gland, Anterior / Q000254:growth & development*; D051381:Rats; D011919:Rats, Inbred Strains; D012970:Sodium Glutamate
D013006:Growth Hormone; D013007:Growth Hormone-Releasing Hormone; D012970:Sodium Glutamate