Micro anion exchange chromatography of carbohydrate-deficient transferrin in serum in relation to alcohol consumption (Swedish Patent 8400587-5).
A new simplified and rapid method for detection and quantitation of "carbohydrate-deficient transferrin" in serum is described. The method is based on isocratic anion exchange chromatography of isotransferrins in disposable microcolumns followed by a double antibody transferrin radioimmune assay. This technique, which separates all transferrin components isoelectric above pH 5.65, showed a very good reproducibility and accuracy with a coefficient of variation between 5 and 9%. 77 alcoholic patients could be clearly separated from 80 healthy "normal consumers" and 33 total abstainers with a specificity of 100% and a sensitivity of 91%. The values were significantly correlated to the amount of alcohol consumed during the latest month, and declined in abstaining alcoholics with a mean biological half-life of 17 days. Elevated levels occasionally appeared in healthy individuals after daily consumption of 60 g of ethanol during a 10-day period. In a sample of 187 patients with nonalcohol-related conditions only 2% false-positive values were found. This method is suggested as a potential tool for detecting and monitoring alcohol abuse.
D000293:Adolescent; D000328:Adult; D000368:Aged; D000428:Alcohol Drinking; D000437:Alcoholism / Q000097:blood*; D002852:Chromatography, Ion Exchange; D005260:Female; D006801:Humans; D005779:Immunodiffusion; D007525:Isoelectric Focusing; D008297:Male; D008875:Middle Aged; D011863:Radioimmunoassay; D014168:Transferrin / Q000032:analysis*
D014168:Transferrin