Induction of interferon-gamma production and Ia expression by interleukin 1 in bone marrow culture cells.
Colony-stimulating factor-induced bone marrow (BM) cultures are a good source of antigen-presenting macrophages. However, they failed to present antigen to T cell clones when antigen was introduced as a short pulse only. Adding interleukin 1 (IL1) to BM culture cells before antigen pulse restored their antigen-presenting activity concomitant to a 2-10-fold increase in Ia antigenicity. We performed a series of experiments to test the mechanism of this IL1-induced activation. Our findings suggest that IL1 influences Ia expression and antigen-specific T cell proliferation by inducing interferon-gamma (IFN-gamma) production. IFN-gamma is produced in this system probably by residual Thy-1-positive cells in the BM cell culture.
D000818:Animals; D001853:Bone Marrow / Q000276:immunology*; D002478:Cells, Cultured; D003115:Colony-Stimulating Factors / Q000276:immunology; D005260:Female; D000949:Histocompatibility Antigens Class II / Q000032:analysis*; D007371:Interferon-gamma / Q000032:analysis / Q000096:biosynthesis*; D007375:Interleukin-1 / Q000276:immunology*; D007739:L Cells / Q000166:cytology; D008213:Lymphocyte Activation; D051379:Mice; D008809:Mice, Inbred C3H; D008815:Mice, Inbred Strains; D013601:T-Lymphocytes / Q000276:immunology*
D003115:Colony-Stimulating Factors; D000949:Histocompatibility Antigens Class II; D007375:Interleukin-1; D007371:Interferon-gamma