Simultaneous measurement of urinary and plasma norepinephrine, epinephrine, dopamine, dihydroxyphenylalanine, and dihydroxyphenylacetic acid by coupled-column high-performance liquid chromatography on C8 and C18 stationary phases.
This paper describes a simple and sensitive method for measuring norepinephrine, epinephrine, dopamine, dihydroxyphenylalanine, and dihydroxyphenylacetic acid in urine and plasma. This method involves a two-stage frontal-chromatographic clean-up, followed by coupled minibore high-performance liquid chromatography with electrochemical detection. The use of phenylboronate instead of alumina yields recoveries in the range of 80-90% for all the catecholamines, dihydroxyphenylalanine and dihydroxyphenylacetic acid without interferences. The interassay coefficient of variation for the measurement of these compounds was less than 12%.
D015102:3,4-Dihydroxyphenylacetic Acid / Q000032:analysis / Q000097:blood / Q000652:urine; D002395:Catecholamines / Q000032:analysis* / Q000097:blood / Q000652:urine; D002851:Chromatography, High Pressure Liquid; D004295:Dihydroxyphenylalanine / Q000032:analysis / Q000097:blood / Q000652:urine; D004298:Dopamine / Q000032:analysis / Q000097:blood / Q000652:urine; D004837:Epinephrine / Q000032:analysis / Q000097:blood / Q000652:urine; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009638:Norepinephrine / Q000032:analysis / Q000097:blood / Q000652:urine; D013048:Specimen Handling
D002395:Catecholamines; D015102:3,4-Dihydroxyphenylacetic Acid; D004295:Dihydroxyphenylalanine; D004298:Dopamine; D009638:Norepinephrine; D004837:Epinephrine