title: Age-dependent variations of intralysosomal enzyme release from human PMN leukocytes under various stimuli.
abstract: The intralysosomal beta glucuronidase and elastase release from polymorphonuclear leukocytes (PMNL) of young and aged male subjects were determined after 60-min incubation with 10 micrograms/ml Cytochalasin B (CB), 10(-6) M of Ca ionophore A 23187 and various concentrations of human low density lipoprotein (LDL). The beta glucoronidase secretion was triggered by both A 23187 and LDL; however, no significant differences were found between the enzyme release from PMNLs of young and aged subjects. In contrast, a marked elastase release was triggered in the young group only by LDL, whereas in the aged group, all of the applied drugs induced a significant elastase release. LDL caused the most dramatic enzyme release from PMNLs of aged males. It was concluded that the release of PMNL-elastase after LDL incorporation as well as by CB and Ca ionophore stimulation may be an age-related process.
mesh_terms: D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000368:Aged; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000494:pharmacology; D003571:Cytochalasin B / Q000494:pharmacology; D005110:Extracellular Space / Q000032:analysis; D005966:Glucuronidase / Q000378:metabolism*; D006801:Humans; D007424:Intracellular Fluid / Q000032:analysis; D008077:Lipoproteins, LDL / Q000494:pharmacology; D008247:Lysosomes / Q000201:enzymology*; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D010196:Pancreatic Elastase / Q000378:metabolism*
chemical_list: D008077:Lipoproteins, LDL; D000001:Calcimycin; D003571:Cytochalasin B; D005966:Glucuronidase; D010196:Pancreatic Elastase; D002118:Calcium