Combined chemoseparation and immunoseparation of clonogenic T lymphoma cells from human bone marrow using 2'-deoxycoformycin, deoxyadenosine, 3A1 monoclonal antibody, and complement.
Chemoseparation and immunoseparation techniques have been combined to eliminate malignant clonogenic T lymphoma cells from human bone marrow. Incubation with 5 microM 2'-deoxycoformycin and 500 microM deoxyadenosine has eliminated 2 logs of HSB-2 T lymphoma cells from a 20-fold excess of irradiated human bone marrow. Multiple incubations with 3A1 antibody and rabbit complement eliminated approximately 2 logs of HSB-2 cells from similar mixtures. Used in combination, the 2 techniques eliminated up to 4 logs of T lymphoma cells. Incubation of normal human bone marrow under similar conditions failed to affect growth of granulocyte-macrophage colony-forming cell units, burst-forming erythroid units, or multipotential erythroid-granulocyte-megakaryocyte-macrophage colony-forming hematopoietic progenitor cells units.
D000911:Antibodies, Monoclonal / Q000276:immunology*; D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface / Q000032:analysis; D001853:Bone Marrow / Q000473:pathology*; D016026:Bone Marrow Transplantation; D002460:Cell Line; D002469:Cell Separation; D003070:Coformycin / Q000031:analogs & derivatives / Q000494:pharmacology*; D003165:Complement System Proteins / Q000276:immunology*; D003839:Deoxyadenosines / Q000494:pharmacology*; D006412:Hematopoietic Stem Cells; D006801:Humans; D008223:Lymphoma / Q000276:immunology / Q000473:pathology*; D015649:Pentostatin; D012263:Ribonucleosides / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000276:immunology; D014410:Tumor Stem Cell Assay
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface; D003839:Deoxyadenosines; D012263:Ribonucleosides; D003070:Coformycin; D015649:Pentostatin; D003165:Complement System Proteins