Reaction of discoidal complexes of apolipoprotein A-I and various phosphatidylcholines with lecithin cholesterol acyltransferase. Interfacial effects.
Complexes of phospholipids-apolipoprotein A-I-cholesterol, containing various bulk phosphatidylcholines or a matrix of the ether analog of 1-palmitoyl 2-oleoyl phosphatidylcholine including test phosphatidylcholines were used as substrates for human lecithin-cholesterol acyltransferase. The enzymatic reaction rates for both series of complexes were determined as a function of temperature, particle concentration, neutral salt concentration, and the type of anion present in solution. The kinetic results support the hypothesis that phospholipids, in discoidal complexes, modulate the reaction rates by molecular effects at the active site, but also by interfacial effects on the interaction of the enzyme with the particles. The relevant interfacial parameters are the lipid packing at the interface and the structure of apolipoprotein A-I.
D000838:Anions; D016632:Apolipoprotein A-I; D001054:Apolipoproteins A / Q000378:metabolism*; D002784:Cholesterol / Q000378:metabolism; D005454:Fluorescence Polarization; D006801:Humans; D007700:Kinetics; D008081:Liposomes / Q000378:metabolism; D008560:Membrane Fluidity; D009994:Osmolar Concentration; D010316:Particle Size; D007862:Phosphatidylcholine-Sterol O-Acyltransferase / Q000378:metabolism*; D010713:Phosphatidylcholines / Q000378:metabolism*; D011487:Protein Conformation; D012965:Sodium Chloride / Q000494:pharmacology; D013050:Spectrometry, Fluorescence; D013379:Substrate Specificity; D013816:Thermodynamics
D000838:Anions; D016632:Apolipoprotein A-I; D001054:Apolipoproteins A; D008081:Liposomes; D010713:Phosphatidylcholines; D012965:Sodium Chloride; D002784:Cholesterol; D007862:Phosphatidylcholine-Sterol O-Acyltransferase; C028694:1-palmitoyl-2-oleoylphosphatidylcholine