The mode of anchoring and precursor forms of sucrase-isomaltase and maltase-glucoamylase in chicken intestinal brush-border membrane. Phylogenetic implications.
Chicken intestinal sucrase-isomaltase and maltase-glucoamylase have been isolated in their intact form by detergent solubilization and characterized as to their subunit composition and mode of anchoring in the brush-border membrane. Both are heterodimeric enzyme complexes composed of two subunits each of approximately 140 and 130 kDa. Contrary to the mammalian sucrase-isomaltase, chicken isomaltase was identified as the smaller of the two subunits. As was shown by hydrophobic labeling, only one of the two subunits in each heterodimer is anchored in the bilayer, the smaller 130 kDa isomaltase subunit of the sucrase-isomaltase complex, and the larger 140 kDa subunit of the maltase-glucoamylase complex. Both preparations contain a high-molecular weight polypeptide of approximately 250 kDa which in the case of sucrase-isomaltase could be identified by peptide mapping as a single-chain precursor not (yet) proteolytically processed to the final heterodimer. These first data on the mode of membrane anchoring of non-mammalian glycosidases indicate that they are synthesized, inserted into the membrane, and processed in ways similar to the mammalian enzymes. The fundamental unity between avian and mammalian sucrase-isomaltases suggests that the partial gene duplication of an ancestral isomaltase gene and the subsequent mutation of one of the active sites resulting in pro-sucrase-isomaltase has occurred prior to the separation of mammals from reptiles, i.e. more than 300 million years ago.
D000818:Animals; D002462:Cell Membrane / Q000201:enzymology / Q000648:ultrastructure; D002645:Chickens / Q000378:metabolism*; D005087:Glucan 1,4-alpha-Glucosidase / Q000032:analysis*; D005959:Glucosidases / Q000032:analysis*; D006026:Glycoside Hydrolases / Q000032:analysis*; D007421:Intestine, Small / Q000201:enzymology*; D008871:Microvilli / Q000201:enzymology*; D009834:Oligo-1,6-Glucosidase / Q000032:analysis*; D011487:Protein Conformation; D011498:Protein Precursors / Q000032:analysis*; D013393:Sucrase / Q000032:analysis*
D011498:Protein Precursors; D005959:Glucosidases; D006026:Glycoside Hydrolases; D009834:Oligo-1,6-Glucosidase; D005087:Glucan 1,4-alpha-Glucosidase; D013393:Sucrase