Primary structure of a new tetraantennary glycan of the N-acetyllactosaminic type isolated from human factor VIII/von Willebrand factor.
N-Glycosidically linked glycopeptides released by mild alkaline treatment of human factor VIII/von Willebrand factor (FVIII/vWF) were fractionated by serial affinity chromatography on columns of Sepharose linked to concanavalin A (ConA) and Lens culinaris agglutinin (LCA). The fraction which is not retained on ConA-Sepharose, but eluted from LCA-Sepharose contains a pure minor glycopeptide which was structurally analysed. Based on the results of methylation analysis and 500-MHz 1H-NMR spectroscopy, the following structure is proposed: (Formula: see text).
D000595:Amino Acid Sequence; D002846:Chromatography, Affinity; D005169:Factor VIII / Q000032:analysis*; D006020:Glycopeptides / Q000032:analysis; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D011134:Polysaccharides / Q000032:analysis*; D014841:von Willebrand Factor / Q000032:analysis*
D006020:Glycopeptides; D011134:Polysaccharides; D014841:von Willebrand Factor; D005169:Factor VIII