The activity of 1-naphthol-UDP-glucuronosyltransferase in the brain.
Cerebral microsomes catalysed efficiently the glucuronidation of 1-naphthol, this formation of glucuronide being activated by treatment with Triton X-100 or digitonin. Activated microsomes from the brain of the rat conjugated 1-naphthol with an apparent Km of 95 microM and a Vmax of 5.47 nmol/hr mg protein at 30 degrees C. Microsomal uridine diphosphate (UDP)-glucuronosyltransferase activity in brain towards 1-naphthol was not significantly induced by pretreatment of animals with 3-methylcholanthrene or phenobarbital. These data suggest that UDP-glucuronosyltransferases in brain are different from the hepatic enzymes with regard to biochemical parameters and in response to inducers of drug metabolism. The hepatic UDP-glucuronosyltransferase deficiency in Gunn rats was also observed in the brain.
D000818:Animals; D001921:Brain / Q000201:enzymology*; D014453:Glucuronosyltransferase / Q000378:metabolism*; D006168:Guinea Pigs; D007700:Kinetics; D008297:Male; D008861:Microsomes / Q000201:enzymology; D009284:Naphthols / Q000378:metabolism; D011817:Rabbits; D051381:Rats; D011911:Rats, Gunn; D011919:Rats, Inbred Strains; D013045:Species Specificity
D009284:Naphthols; C029350:1-naphthol; D014453:Glucuronosyltransferase