Comparison of the results of card, rivanol, complement-fixation, and milk ring tests with the isolation rate of Brucella abortus from cattle.
Specimens from 4,553 cows were examined by card, Rivanol, and complement-fixation (CF) tests and bacteriologic culture. A ring test was performed on milk from 1,003 of these cows. The isolation rate of Brucella abortus correlated directly with antibody titers, and the field strain predominated in adult-vaccinated cows when the Rivanol test titer was greater than + 100 and the CF test titer was greater than 4 + 40. The CF test had the best balance of sensitivity and specificity in adult-vaccinated cows. The false-negative rate for the Rivanol and CF tests was higher in nonadult-vaccinated cows.
D000818:Animals; D000891:Anti-Infective Agents, Local; D001431:Bacteriological Techniques; D002003:Brucella abortus / Q000302:isolation & purification*; D002007:Brucellosis, Bovine / Q000175:diagnosis*; D002417:Cattle; D003168:Complement Fixation Tests; D004975:Ethacridine / Q000031:analogs & derivatives; D005260:Female; D011233:Precipitin Tests; D012698:Serologic Tests
D000891:Anti-Infective Agents, Local; D004975:Ethacridine