Arylsulphatase C activity in leukocytes of patients and carriers of X-linked ichthyosis.
In Triton X-100 solubilized leukocytes of 17 patients and 8 obligate carriers of X-linked recessive ichthyosis (XLI) the activity of arylsulphatase C (ASC) was determined and expressed as the ratio to beta-galactosidase activity. The ASC/beta-gal ratio of XLI patients is markedly decreased (range 0.07-0.48) in comparison to the corresponding control group of males (range 1.3-2.7). The enzyme ratios of 8 obligate carriers of XLI are decreased (range 0.90-1.9) in comparison to the normal females (2.13-5.52). These results indicate that the determination of the enzyme ratio of ASC/beta-gal in Triton X-100 solubilized leukocytes is a sensitive test for biochemical identification of patients and probably of carriers of XLI.
D001192:Arylsulfatases / Q000097:blood* / Q000302:isolation & purification; D005260:Female; D006579:Heterozygote; D006801:Humans; D007057:Ichthyosis / Q000201:enzymology* / Q000235:genetics; D007962:Leukocytes / Q000201:enzymology*; D008297:Male; D017830:Octoxynol; D011092:Polyethylene Glycols / Q000494:pharmacology; D043266:Steryl-Sulfatase; D013429:Sulfatases / Q000097:blood*; D001616:beta-Galactosidase / Q000032:analysis
D011092:Polyethylene Glycols; D017830:Octoxynol; D013429:Sulfatases; D001192:Arylsulfatases; D043266:Steryl-Sulfatase; D001616:beta-Galactosidase