Interferon-gamma-induced expression of class I HLA antigens on K-562 cells grown in serum-free medium.
Human interferon-gamma (IFN-gamma), encoded in Escherichia coli by recombinant human DNA, induces the expression of HLA antigens in the pluripotent hematopoietic stem cell line K-562 grown in serum-free growth medium. Expression was noted in 90% of the cells within 4 days and there was a high density of expression per cell, as determined by cytofluorography. Upon subculture, the cells rapidly lose their ability to express HLA, indicating that the continued presence of IFN-gamma is necessary for the expression. Reinduction of expression with IFN-gamma occurs in a high percentage of cells (up to 90%), which is comparable to the induction in freshly treated control cells.
D002460:Cell Line; D003470:Culture Media; D006680:HLA Antigens; D006412:Hematopoietic Stem Cells / Q000187:drug effects / Q000276:immunology; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*
D003470:Culture Media; D006680:HLA Antigens; D007371:Interferon-gamma