Two-monoclonal-antibody "sandwich"-type assay of human lutropin, with no cross reaction with choriogonadotropin.
We have developed a sensitive, specific, noncompetitive sandwich-type assay for human lutropin (hLH). Two monoclonal antibodies are used, and there is no cross reaction with human choriogonadotropin (hCG) or human follitropin (hFSH), and little or none with human thyrotropin (hTSH). There also is no reaction with the free beta chains of hLH and hCG. The detection limit is less than 0.5 int. units of hLH per liter of serum, and the dose-response curve is linear between 0 and 10 int. units/L. The intra-assay CV averaged 5.4% at low doses of hLH; the interassay CV averaged 12.5%.
D000911:Antibodies, Monoclonal; D000917:Antibody Formation; D000918:Antibody Specificity; D006063:Chorionic Gonadotropin / Q000032:analysis*; D003429:Cross Reactions; D005640:Follicle Stimulating Hormone / Q000032:analysis; D006801:Humans; D007158:Immunologic Techniques; D007202:Indicators and Reagents; D007986:Luteinizing Hormone / Q000032:analysis*; D013972:Thyrotropin / Q000032:analysis
D000911:Antibodies, Monoclonal; D006063:Chorionic Gonadotropin; D007202:Indicators and Reagents; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D013972:Thyrotropin