Adherent spleen cell production of E series prostaglandins in rats bearing variants of the R3327 Dunning prostatic adenocarcinoma: effect of cyclophosphamide.
Prostaglandins of the E series (PGE) have been implicated in many facets of immunoregulation, as well as having a possible role in metastatic dissemination. Variant sublines of the Dunning R3327 rat prostatic adenocarcinoma, differing in growth rate, hormonal responsiveness and in propensity for metastasis, were carried in Fisher X Copenhagen F1 animals. Adherent spleen cells were assayed in vitro for their ability to convert arachidonic acid to prostaglandins of the E series. These glass adherent cells presumably include the monocytic and T cell populations which have been implicated as being immunoregulatory. The results indicated that those spleen cells obtained from animals carrying the metastatic R3327-MAT-LyLu subline tumor converted more arachidonic acid to PGE's than cells derived from animals bearing non-metastatic sublines. Cyclophosphamide therapy did not alter such conversion. Multiple regulatory mechanisms for prostaglandin metabolism are suggested.
D000230:Adenocarcinoma / Q000188:drug therapy / Q000276:immunology / Q000378:metabolism*; D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism; D002448:Cell Adhesion; D003520:Cyclophosphamide / Q000494:pharmacology*; D007107:Immune System / Q000503:physiopathology; D008297:Male; D009362:Neoplasm Metastasis; D011458:Prostaglandins E / Q000096:biosynthesis* / Q000502:physiology; D011471:Prostatic Neoplasms / Q000188:drug therapy / Q000276:immunology / Q000378:metabolism*; D051381:Rats; D013154:Spleen / Q000378:metabolism / Q000473:pathology
D001095:Arachidonic Acids; D011458:Prostaglandins E; D016718:Arachidonic Acid; D003520:Cyclophosphamide