In vivo clearance of antibody-sensitized human drug carrier erythrocytes.
Antibody coating of resealed drug carrier erythrocytes may be useful for drug targeting to the reticuloendothelial system. We have investigated the survival in the circulation of anti-Rh antibody (IgG anti-D)-coated autologous erythrocytes loaded with gentamicin by hypoosmotic dialysis. Five subjects were injected with 15.2 +/- 0.4 ml and five additional subjects with 62.8 +/- 1.5 ml carrier cells. Survival of the cells was monitored by intraerythrocytic gentamicin concentration in blood. In the first subject group initial t1/2 was 0.21 +/- 0.06 hours and terminal t1/2 was 1.71 +/- 0.36 hours. In the second group initial t1/2 was 0.59 +/- 0.21 hours followed by a slow phase with a t1/2 of 89 +/- 28 hours. Results indicate that rapid drug delivery to the reticuloendothelial system by antibody-sensitized carrier erythrocytes is possible, but small volumes of erythrocytes seem more efficient.
D000328:Adult; D000906:Antibodies; D004304:Dosage Forms; D004912:Erythrocytes / Q000378:metabolism*; D005839:Gentamicins / Q000097:blood; D006801:Humans; D007700:Kinetics; D008297:Male; D012157:Mononuclear Phagocyte System / Q000187:drug effects; D012204:Rh-Hr Blood-Group System / Q000276:immunology*
D000906:Antibodies; D004304:Dosage Forms; D005839:Gentamicins; D012204:Rh-Hr Blood-Group System