Identification of Brucella abortus in formalin-fixed, paraffin-embedded tissues of cows, goats, and mice with an avidin-biotin-peroxidase complex immunoenzymatic staining technique.
An avidin-biotin-peroxidase complex immunoenzymatic staining technique was evaluated for light microscopic detection of Brucella organisms in formalin-fixed, paraffin-embedded tissues. Tissues from cows, goats, and mice inoculated with B abortus strain 2308 were examined, using rabbit antiserum to Brucella cell surface protein as primary antibody. Stained organisms were identified histologically in tissue sections containing B abortus, as detected by bacteriologic examination of duplicate nonprocessed tissue samples. Bacillus sp, Corynebacterium pyogenes, Escherichia coli, Listeria monocytogenes, Pasteurella haemolytica, P multocida, Salmonella typhimurium, Staphylococcus sp, and Streptococcus sp did not stain with this system, using anti-Brucella cell surface protein primary antibody, thus indicating specificity for Brucella organisms.
D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis; D002003:Brucella abortus / Q000276:immunology / Q000302:isolation & purification*; D002006:Brucellosis / Q000382:microbiology / Q000662:veterinary*; D002007:Brucellosis, Bovine / Q000382:microbiology*; D002417:Cattle; D005260:Female; D006041:Goats; D007124:Immunoenzyme Techniques; D051379:Mice; D010920:Placenta / Q000382:microbiology; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000382:microbiology / Q000662:veterinary; D013154:Spleen / Q000382:microbiology
D000942:Antigens, Bacterial