title: Decarboxylation of DL-2,4-dihydroxyphenylalanine (2,4-DOPA) to 2,4-dihydroxyphenylethylamine in mouse striatal slices.
abstract: Decarboxylation of an unnatural analogue of DOPA by brain aromatic amino acid decarboxylase was measured by HPLC with electrochemical detection. Incubation of striatal slices with DL-2,4-dihydroxyphenylalanine (2,4-DOPA) resulted in biosynthesis and accumulation of 2,4-dihydroxyphenylethylamine, which was completely blocked by carbidopa. 2,4-DOPA induced a significant decrease in endogenous dopamine, but the loss was not prevented by carbidopa. Since 2,4-DOPA is a potential pro-drug for melanoma chemotherapy, further evaluation of its neurobiologic properties is needed.
mesh_terms: D000818:Animals; D065105:Aromatic Amino Acid Decarboxylase Inhibitors; D002230:Carbidopa / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D003342:Corpus Striatum / Q000378:metabolism*; D003653:Decarboxylation; D004295:Dihydroxyphenylalanine / Q000031:analogs & derivatives* / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D010627:Phenethylamines / Q000378:metabolism*
chemical_list: D065105:Aromatic Amino Acid Decarboxylase Inhibitors; D010627:Phenethylamines; C053215:2,4-dihydroxyphenylethylamine; C053214:2,4-dihydroxyphenylalanine; D004295:Dihydroxyphenylalanine; D002230:Carbidopa