A novel synthetic vitamin A-like compound (a polyprenoic acid derivative, E-5166) inhibits the release of arachidonic acid stimulated by epidermal growth factor.
Little is known about the mechanisms of anti-inflammatory activity of retinoids. A new synthetic vitamin A-like compound (polyprenoic acid derivative, E-5166) has a strong in vitro binding affinity to intracellular binding proteins for acidic retinoids. In order to elucidate the anti-inflammatory activity of E-5166, we studied the effect of E-5166 on the epidermal growth factor (EGF)-stimulated arachidonic acid (AA) release of pig epidermis. E-5166 significantly inhibited the EGF-stimulated AA release and this inhibitory effect of E-5166 required a longer incubation than hydrocortisone did. Furthermore, E-5166 inhibited the EGF-stimulated phosphatidylinositol (PI) turnover of pig epidermis. These results indicate that E-5166 inhibited the EGF-stimulated AA release through the inhibition of the EGF-stimulated PI turnover.
D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000037:antagonists & inhibitors* / Q000378:metabolism; D000001:Calcimycin / Q000494:pharmacology; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D010716:Phosphatidylinositols / Q000096:biosynthesis; D012867:Skin / Q000378:metabolism; D013268:Stimulation, Chemical; D013552:Swine; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014212:Tretinoin / Q000031:analogs & derivatives* / Q000494:pharmacology; D014801:Vitamin A / Q000494:pharmacology*
D001095:Arachidonic Acids; D010716:Phosphatidylinositols; D014801:Vitamin A; D016718:Arachidonic Acid; D000001:Calcimycin; D014212:Tretinoin; D004815:Epidermal Growth Factor; C034534:3,7,11,15-tetramethyl-2,4,6,10,14-hexadecapentaenoic acid; D013755:Tetradecanoylphorbol Acetate