title: Lectin binding and inhibition studies reveal the importance of D-glucose, D-mannose and N-acetylglucosamine during early sexual development of Dictyostelium discoideum.
abstract: Fluorescein-conjugated and non-conjugated lectins were used to determine which surface sugars are involved in the early events of sexual (macrocyst) development in Dictyostelium discoideum. Only zygote giant cells showed unique binding of FITC-WGA and FITC-PNA while all cell types (amoebae, gametes, binucleates, giant cells) showed identical patterns of FITC-Con A, -Gorse and -RCA II binding. In spite of its non-selective labelling of all cell types, Con A inhibited macrocyst formation. The temporal addition of Con A with and without specific hapten sugars indicates the importance of both D-mannose and D-glucose in phagocytosis and, possibly, cell fusion. WGA also inhibited macrocyst formation. Varying the time of addition of the lectin plus/minus its primary hapten sugar implicates N-acetylglucosamine as being important in cell fusion. Neither Gorse, RCA II nor PNA had any detectable inhibitory effects on macrocyst development leaving the appearance of increased PNA receptors at the giant cell surface as an enigma.
mesh_terms: D000117:Acetylglucosamine / Q000378:metabolism; D002448:Cell Adhesion; D002454:Cell Differentiation; D002459:Cell Fusion; D004023:Dictyostelium / Q000254:growth & development*; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D005947:Glucose / Q000378:metabolism; D037102:Lectins; D008358:Mannose / Q000378:metabolism; D010587:Phagocytosis; D012723:Sex; D013499:Surface Properties; D013861:Thiocyanates
chemical_list: D005452:Fluoresceins; D037102:Lectins; D013861:Thiocyanates; D016650:Fluorescein-5-isothiocyanate; D005947:Glucose; D008358:Mannose; D000117:Acetylglucosamine