Immunochemical analysis of porcine cardiac C-protein.
C-protein has been isolated from pig heart and its immunochemical properties studied. It is extracted with myosin, and separated from the myosin on a DEAE-Sephadex column. The amount of C-protein recovered from crude myosin is approx. 3.5%. The molecular weight of C-protein is 150,000. Anti-C-protein serum reacts with crude myosin and purified C-protein but not with purified myosin in immunodiffusion plates. Cardiac C-protein does not react with anti-skeletal white muscle C-protein serum. Immunoblotting experiments show that anti-cardiac C-protein serum reacts with a Mr = 150,000 component in myofibrils or crude myosin. C-protein is located in the A-band, except the M-line region, of the myofibrils. These results indicate that C-protein is an intrinsic component of the thick filaments in pig heart myofibrils.
D000818:Animals; D000936:Antigen-Antibody Complex; D002352:Carrier Proteins; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D007106:Immune Sera; D005779:Immunodiffusion; D008970:Molecular Weight; D009124:Muscle Proteins / Q000032:analysis* / Q000276:immunology; D009206:Myocardium / Q000032:analysis*; D013552:Swine; D000099163:Myosin Binding Protein C
D000936:Antigen-Antibody Complex; D002352:Carrier Proteins; D007106:Immune Sera; D009124:Muscle Proteins; D000099163:Myosin Binding Protein C