Distribution of Gc protein (vitamin D binding protein) on the surfaces of normal human lymphocytes and leukemic lymphocytes.
Distribution of membrane-bound Gc protein (mGc) in normal lymphocyte subpopulations and leukemic lymphocytes was studied using a rabbit antiserum specific to Gc protein. Forty to fifty percent of the normal peripheral blood mononuclear cells were stained with anti-Gc antiserum by membrane immunofluorescence, and almost all of the B cells (B1+) and NK cells (Leu 11+) were mGc-positive. Only a few resting T cells (T3+) reacted with anti-Gc, but mGc appeared in a significant number of the T cells after activation by PHA. The distribution of mGc on leukemic lymphocytes of various types nearly corresponded to that in normal lymphocyte subpopulations.
D001402:B-Lymphocytes / Q000032:analysis; D005455:Fluorescent Antibody Technique; D006801:Humans; D007694:Killer Cells, Natural / Q000032:analysis; D007938:Leukemia / Q000097:blood*; D008214:Lymphocytes / Q000032:analysis*; D009000:Monocytes / Q000032:analysis; D010835:Phytohemagglutinins / Q000494:pharmacology; D013499:Surface Properties; D013601:T-Lymphocytes / Q000032:analysis; D014018:Tissue Distribution; D014809:Vitamin D-Binding Protein / Q000097:blood*
D010835:Phytohemagglutinins; D014809:Vitamin D-Binding Protein