Chromosome marker and enhanced expression of c-Ha-ras in a DMBA-induced erythroleukemia cell line (D5A1).
Oncogene activation induced by chromosomal changes is now regarded as one of the most important phenomena during carcinogenesis. We have reported c-abl activation in a rat leukemia cell line K3D, caused by a secondary chromosomal translocation. Another erythroblastic leukemia cell line D5A1, originally derived from a leukemia induced by 7,12-dimethylbenz(a)anthracene (DMBA) in a Long-Evans rat, is characterized by a marker chromosome 1q+, which also probably occurred as a secondary change. In this cell line, the transcription level of Ha-ras related mRNA increased compared with other cell lines. By the in situ hybridization technique, the c-Ha-ras locus was assigned to 1q43 and the breakpoint 1q+. Because the breakpoint was so near the c-Ha-ras locus on the chromosome, the present system may provide a model of activation of the c-Ha-ras gene brought about by chromosomal translocation.
D015127:9,10-Dimethyl-1,2-benzanthracene; D000818:Animals; D002460:Cell Line; D002871:Chromosome Banding; D005819:Genetic Markers; D007621:Karyotyping; D004915:Leukemia, Erythroblastic, Acute / Q000139:chemically induced / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D011519:Proto-Oncogenes; D051381:Rats; D014178:Translocation, Genetic
D005819:Genetic Markers; D015127:9,10-Dimethyl-1,2-benzanthracene