Release of a lectin from a fatty acid auxotroph of Saccharomyces cerevisiae grown in presence of oleic acid.
The unsaturated fatty acid-requiring mutant KD 115 of Saccharomyces cerevisiae secretes a lectin when grown in presence of oleic acid. This lectin is homogeneous on PAGE at pH 8.3, has an approximate molecular weight of 320,000, pI of 4.2 and contains about 60% sugar. It agglutinates chicken and different mammalian erythrocytes, but lyses rabbit red cells only. It is D-galactose-specific. To our knowledge, this is the first report of a hemagglutinin from yeast.
D000818:Animals; D002241:Carbohydrates / Q000494:pharmacology; D002645:Chickens; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D044943:Fatty Acid Desaturases / Q000235:genetics / Q000502:physiology*; D005690:Galactose / Q000494:pharmacology; D006041:Goats; D006384:Hemagglutination / Q000187:drug effects; D006461:Hemolysis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D005779:Immunodiffusion; D007525:Isoelectric Focusing; D037102:Lectins / Q000302:isolation & purification / Q000378:metabolism*; D008970:Molecular Weight; D009154:Mutation; D019301:Oleic Acid; D009829:Oleic Acids / Q000494:pharmacology*; D011817:Rabbits; D051381:Rats; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D013230:Stearoyl-CoA Desaturase
D002241:Carbohydrates; D037102:Lectins; D009829:Oleic Acids; D019301:Oleic Acid; D044943:Fatty Acid Desaturases; D013230:Stearoyl-CoA Desaturase; C029627:delta-9 fatty acid desaturase; D005690:Galactose