Enhancement of estradiol-induced DNA synthesis in the anterior pituitary gland by the peripheral-type benzodiazepine receptor ligand Ro 5-4864.
The effects of peripheral (Ro 5-4864) and central-type (Ro 15-1788) benzodiazepine receptor ligands on estrogen-induced DNA synthesis in the rat anterior pituitary gland was investigated. As expected, a single injection of estradiol (250 micrograms per rat) significantly increased the uptake of 3H-thymidine by anterior pituitary cells. Additional treatment with Ro 5-4864 potentiated the effects of estradiol on pituitary DNA synthesis. Under the same experimental conditions, no effect of Ro 15-1788 and sodium valproate, a GABA-transaminase inhibitor, was detected. These findings indicate the involvement of peripheral-type benzodiazepine receptors in the control of anterior pituitary cell proliferation.
D000818:Animals; D001570:Benzodiazepinones / Q000008:administration & dosage*; D004247:DNA / Q000096:biosynthesis*; D004357:Drug Synergism; D004958:Estradiol / Q000008:administration & dosage*; D005442:Flumazenil / Q000008:administration & dosage; D008297:Male; D008938:Mitosis / Q000187:drug effects; D010903:Pituitary Gland, Anterior / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D014635:Valproic Acid / Q000008:administration & dosage
D001570:Benzodiazepinones; C028513:4'-chlorodiazepam; D005442:Flumazenil; D004958:Estradiol; D014635:Valproic Acid; D004247:DNA