Ionophore-stimulated rat basophilic leukemia cells produce PAF-acether.
Ionophore (A23187) stimulated rat basophilic leukemia (RBL-1) cells produce a lipid mediator which caused rabbit platelets to aggregate and which by using platelet-activating factor (PAF)-acether antagonists and high-pressure liquid chromotography was shown to be PAF-acether. Thus RBL-1 cells represent a cell type suitable for studying the coordinated release of three mediators of anaphylaxis: histamine, leukotrienes C4/D4 (slow-reacting substances) and PAF-acether.
D000818:Animals; D001491:Basophils; D000001:Calcimycin / Q000494:pharmacology*; D006168:Guinea Pigs; D007942:Leukemia, Experimental / Q000473:pathology*; D010972:Platelet Activating Factor / Q000378:metabolism*; D010974:Platelet Aggregation; D011817:Rabbits; D051381:Rats
D010972:Platelet Activating Factor; D000001:Calcimycin