title: Comparative study on Lewis lung tumor lines with 'low' and 'high' metastatic capacity. II. Cytochemical and biochemical evidence for differences in glycosaminoglycans.
abstract: The enhanced metastatic capacity of an in vivo selected Lewis lung tumor line (LLT-HH) was correlated with changes in cell-associated glycosaminoglycans (GAG) using ultrastructural cytochemistry, flow cytometry and biochemistry. The increase in highly sulphated GAG content on the cell membrane of LLT-HH cells compared to the parent LLT cells was demonstrated cytochemically. Using in vitro [3H]glucosamine labelling of GAG components it was shown that the LLT-HH cells were characterized by a high production of heparan sulphate while the parent LLT line had a high hyaluronic acid-chondroitin sulphate production. The high metastatic phenotype is accompanied by an altered production of cell-associated GAGs.
mesh_terms: D000818:Animals; D002277:Carcinoma / Q000473:pathology / Q000503:physiopathology*; D002448:Cell Adhesion; D002455:Cell Division; D002478:Cells, Cultured; D002809:Chondroitin Sulfates / Q000378:metabolism; D005434:Flow Cytometry; D006025:Glycosaminoglycans / Q000502:physiology*; D006497:Heparan Sulfate / Q000378:metabolism; D006651:Histocytochemistry; D006820:Hyaluronic Acid / Q000378:metabolism; D051379:Mice; D009362:Neoplasm Metastasis
chemical_list: D002809:Chondroitin Sulfates; D006025:Glycosaminoglycans; D006497:Heparan Sulfate; D006820:Hyaluronic Acid