Inactivation of skeletal glycogen synthetase by diethylpyrocarbonate.
Glycogen synthetase from skeletal muscle is rapidly inactivated by DEPC. In the presence of the substrate UDPG only 50% of the enzyme activity is lost. The concomitant addition of both UDPG and the allosteric activator glucose-6-phosphate almost completely prevents the inactivation by DEPC. Since glucose-6-phosphate alone does not prevent the inactivation by DEPC, it is concluded that it is effective through a potentiation of the effects of UDPG, possibly through a conformational change of the enzyme.
D000818:Animals; D004047:Diethyl Pyrocarbonate / Q000494:pharmacology*; D005561:Formates / Q000494:pharmacology*; D006006:Glycogen Synthase / Q000037:antagonists & inhibitors*; D007700:Kinetics; D009132:Muscles / Q000201:enzymology*; D011817:Rabbits; D014532:Uridine Diphosphate Glucose / Q000494:pharmacology
D005561:Formates; D006006:Glycogen Synthase; D004047:Diethyl Pyrocarbonate; D014532:Uridine Diphosphate Glucose