CD5 positive B cells in patients with rheumatoid arthritis: phorbol ester mediated enhancement of detection.
CD5 molecules present on human T cells are detectable but weakly expressed on some human B cells. We have increased the sensitivity of their detection by treating the B enriched cells with phorbol myristic acetate (PMA), a tumour promoting agent. The numbers of CD5+ B cells in the blood of patients with rheumatoid arthritis (RA) were higher than in control blood, and after PMA treatment this was statistically significant. CD5+ B cells were also increased in tonsils, lymph nodes, and spleens after PMA activation. There were no significant differences between the percentages of B cells carrying chi or lambda light chains in their expression of CD5 molecules in patients with RA.
D000328:Adult; D000368:Aged; D000944:Antigens, Differentiation, B-Lymphocyte; D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface / Q000276:immunology*; D001172:Arthritis, Rheumatoid / Q000276:immunology*; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D002478:Cells, Cultured; D005260:Female; D005455:Fluorescent Antibody Technique; D006133:Growth Substances / Q000276:immunology*; D006801:Humans; D015847:Interleukin-4; D008198:Lymph Nodes / Q000276:immunology; D008222:Lymphokines / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D014066:Palatine Tonsil / Q000276:immunology; D013154:Spleen / Q000276:immunology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D000944:Antigens, Differentiation, B-Lymphocyte; D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface; D006133:Growth Substances; D008222:Lymphokines; D015847:Interleukin-4; D013755:Tetradecanoylphorbol Acetate