[Demonstration of a TRH precursor in the pancreas of the newborn rat].
This study allows the indirect demonstration of a precursor for TRH in pancreatic extracts of 2-days old rats. The sequential treatment of these extracts with trypsin and carboxypeptidase A is followed by a large increase in Pyroglutamyl Histidine Proline (TRH-OH). The molecular weight of the protein that gives rise to TRH-OH after enzymatic treatment ranges between 30,000 and 40,000 daltons. During ontogenesis. TRH levels decrease earlier and more rapidly than that of TRH-precursor levels. These data suggest that changes in the processing of TRH-precursor play a role in the diminution of TRH concentrations that is observed during the first two weeks of life.
D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism*; D008970:Molecular Weight; D010179:Pancreas / Q000378:metabolism*; D011498:Protein Precursors / Q000378:metabolism*; D011761:Pyrrolidonecarboxylic Acid / Q000031:analogs & derivatives; D051381:Rats; D013973:Thyrotropin-Releasing Hormone / Q000031:analogs & derivatives / Q000378:metabolism*
D011498:Protein Precursors; C020271:thyrotropin-releasing hormone, deamido-; D013973:Thyrotropin-Releasing Hormone; C046418:pro-thyrotropin releasing hormone; D011761:Pyrrolidonecarboxylic Acid
Mise en évidence d'un précurseur pour la TRH dans le pancréas de rat nouveau-né.