13-Hydroxyoctadecadienoic acid (13-HODE) stimulates prostacyclin production by endothelial cells.
The effect of 13-hydroxyoctadecadienoic acid (13-HODE), an endogenous lipoxygenase metabolite of linoleic acid, on prostacyclin production by fetal bovine aortic endothelial cells was evaluated. Time-dependent release of radioimmunoassayable 6KPGF1 alpha in the presence of 13-HODE (10 uM) was stimulated by 39%, 27%, and 34% at 10, 30 and 120 min respectively. 13-HODE (10 uM) had no effect on the conversion of exogenous [14C] arachidonic acid (AA) to prostacyclin. When the effect on AA release was evaluated in [14C] AA prelabeled cells, 13-HODE (10nM) stimulated the release of AA from membrane phospholipids. Analysis of cellular phospholipids revealed a significant decrease in phosphatidylethanolamine. Our results demonstrate that 13-HODE stimulates prostacyclin production by enhancing AA release from phospholipids.
D015121:6-Ketoprostaglandin F1 alpha / Q000378:metabolism; D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism; D002417:Cattle; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D004727:Endothelium / Q000187:drug effects* / Q000378:metabolism; D011464:Epoprostenol / Q000096:biosynthesis*; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000378:metabolism / Q000494:pharmacology*; D008084:Lipoxygenase / Q000378:metabolism; D008563:Membrane Lipids / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism
D001095:Arachidonic Acids; D008041:Linoleic Acids; D008563:Membrane Lipids; D010743:Phospholipids; D016718:Arachidonic Acid; C024348:13-hydroxy-9,11-octadecadienoic acid; D015121:6-Ketoprostaglandin F1 alpha; D019787:Linoleic Acid; D011464:Epoprostenol; D008084:Lipoxygenase