UDPgalactose:glucosylceramide beta 1----4-galactosyltransferase activity in human proximal tubular cells from normal and familial hypercholesterolemic homozygotes.
The activity of a galactosyltransferase (GalT-2) that catalyzes the transfer of galactose from uridinediphosphogalactose to glucosylceramide in cultured normal human proximal tubular (PT) cells was characterized with respect to substrate saturation and metal ion requirements. Using a membrane-bound enzyme source, optimum activity was obtained in the presence of 1.0 mM Mn2+/Mg2+ (1:1) and a detergent mixture, Triton X-100/Cutscum (1:2, v/v), 0.1 mg/ml. The apparent Km values for glucosylceramide and UDP[14C]galactose were 3 microM and 0.5 microM, respectively. The Vmax values for glucosylceramide and UDP[U-14C]galactose were 0.12 nmol/mg protein per 2 h and 173 nmol/mg protein per 2 h, respectively. The purified 14C-labelled product comigrated with authentic lactosylceramide (LacCer) on TLC and HPLC analysis. The presence of a terminal beta-[14C]galactosyl group in the enzymatic product was proved by its cleavage (79%) by beta-galactosidase. Following the development of optimal assay conditions in normal PT cells, GalT-2 activity was next measured in urinary PT cells from homozygous familial hypercholesterolemic (FH) patients previously shown to accumulate large amounts of lactosylceramide. Urinary PT cells from familial hypercholesterolemic homozygous patients contained 35% higher GalT-2 activity as compared to control cells. We speculate that elevated GalT-2 activity may contribute to the storage of LacCer in FH-PT cells.
D015703:Antigens, CD; D002462:Cell Membrane / Q000201:enzymology; D002478:Cells, Cultured; D003902:Detergents / Q000494:pharmacology; D005700:Galactosyltransferases / Q000378:metabolism*; D005963:Glucosylceramides / Q000378:metabolism; D006028:Glycosphingolipids / Q000378:metabolism; D006801:Humans; D006938:Hyperlipoproteinemia Type II / Q000201:enzymology* / Q000652:urine; D007687:Kidney Tubules, Proximal / Q000201:enzymology*; D007700:Kinetics; D007790:Lactosylceramides; D008274:Magnesium / Q000494:pharmacology; D008345:Manganese / Q000494:pharmacology; D014531:Uridine Diphosphate Galactose / Q000378:metabolism; D001616:beta-Galactosidase / Q000378:metabolism
D015703:Antigens, CD; D003902:Detergents; D005963:Glucosylceramides; D006028:Glycosphingolipids; D007790:Lactosylceramides; D014531:Uridine Diphosphate Galactose; D008345:Manganese; C009744:CDw17 antigen; D005700:Galactosyltransferases; C039090:UDPgalactose-glucosylceramide galactosyltransferase; D001616:beta-Galactosidase; D008274:Magnesium