Comparison of in vitro and in vivo effects of different species specific GnRH and their analogs.
Mammalian, salmon and chicken gonadotropin-releasing hormones (mGnRH, sGnRH, cGnRH) and their analogs were synthesized and tested for their ability to stimulate in vitro LH and FSH release from cultured and superfused rat pituitary cells and also their in vivo effect were investigated on the artificial propagation of fishes. The LH and FSH releasing activity of sGnRH, cGnRH and their analogs were lower than the appropriate mammalian ones from cultured rat pituitary cells, but two of the cGnRH analogs showed increased LH and FSH secretory activity from superfused rat pituitary cells compared to the mGnRH. At the same time these two analogs are very potent to stimulate reproductive function of fishes and using these peptides we were able to fulfill the artificial propagation of fishes which could not be artificially propagated before.
D000596:Amino Acids; D000818:Animals; D002645:Chickens; D005640:Follicle Stimulating Hormone / Q000378:metabolism; D007987:Gonadotropin-Releasing Hormone / Q000031:analogs & derivatives* / Q000494:pharmacology; D066298:In Vitro Techniques; D007986:Luteinizing Hormone / Q000378:metabolism; D008322:Mammals; D010903:Pituitary Gland, Anterior / Q000187:drug effects / Q000378:metabolism; D012474:Salmon; D013045:Species Specificity
D000596:Amino Acids; D007987:Gonadotropin-Releasing Hormone; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone