Induction of immunity against infection with Theileria parva (East Coast fever) in cattle using plasma membranes from parasitized lymphoblasts.
Protection against challenge with Theileria parva was conferred on three of four calves given three or four inocula of plasma membranes prepared from 6 to 12 X 10(8) autologous parasitized lymphoblasts from cultured cell lines. In contrast, calves remained susceptible to infection following immunization with membranes prepared from allogeneic parasitized lymphoblasts. Similarly, calves vaccinated with either gamma-irradiated autologous or allogeneic infected cells also died of East Coast fever after challenge. The results raise the possibility of vaccination against T. parva using subcellular material from infected lymphoblasts.
D000818:Animals; D016782:Apicomplexa / Q000254:growth & development / Q000276:immunology; D002417:Cattle; D002460:Cell Line; D002462:Cell Membrane / Q000276:immunology; D005720:Gamma Rays; D007110:Immunity, Active; D007114:Immunization / Q000662:veterinary*; D008214:Lymphocytes / Q000276:immunology* / Q000469:parasitology / Q000528:radiation effects / Q000648:ultrastructure; D013801:Theileriasis / Q000276:immunology*