Hepatic regeneration and metabolism after partial hepatectomy in normal rats: effects of insulin therapy.
The effect of insulin therapy on liver regeneration has been studied in normal fed rats 12, 24 and 48 h after partial hepatectomy. Dry weight of regenerating liver increased between 12 and 48 h after partial hepatectomy and was unaffected by insulin therapy. [6-3H] Thymidine uptake peaked at 24-h (24.7 +/- 2.4% of total liver cells) and insulin treatment had no additional effect. At 12-h after partial hepatectomy, hepatic [ATP] was decreased 15%, while [ADP] and [AMP] were increased 47% and 83% respectively compared with sham-operated animals. Partial hepatectomy also caused an increase in hepatic [triglyceride], a decrease in hepatic [glycogen] and an increase in the levels of glucose and several glycolytic intermediates. The hepatic redox ratios, [lactate]:[pyruvate] and [3-hydroxybutyrate]:[acetoacetate], were elevated. Insulin therapy had only minor effects on hepatic adenine nucleotide levels, intermediary metabolite concentrations or intrahepatic redox ratios after partial hepatectomy. These findings suggest a decreased hepatic intracellular energy state in regenerating liver; insulin therapy in normal rats does not influence this metabolic change nor the regenerative response.
D000227:Adenine Nucleotides / Q000378:metabolism; D000818:Animals; D004247:DNA / Q000096:biosynthesis; D005947:Glucose / Q000378:metabolism; D006003:Glycogen / Q000378:metabolism; D006498:Hepatectomy; D007328:Insulin / Q000627:therapeutic use*; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D008115:Liver Regeneration / Q000187:drug effects*; D008297:Male; D009929:Organ Size; D051381:Rats; D011919:Rats, Inbred Strains; D013936:Thymidine / Q000378:metabolism; D014867:Water / Q000032:analysis
D000227:Adenine Nucleotides; D007328:Insulin; D014867:Water; D006003:Glycogen; D004247:DNA; D005947:Glucose; D013936:Thymidine