Dietary potentiation of the antifertility effects of 5-thio-D-glucose in male rats.
Male rats of proven fertility were fed the following diets for 28 d either with or without 0.075% 5-thioglucose (5-THG): AIN-76 diet (A76): a diet with 13% casein, 2% glucose and the balance of the calories as free corn oil fatty acids from corn oil (2G); and a similar diet, isocaloric with 2G, with the glucose level increased to 20% (20G). The diets alone without 5-THG had no influence on any of the parameters measured. Body weight gain was lower in rats fed diets containing 5-THG than in those fed diets without 5-THG. In rats fed A76, the only 5-THG effects on male reproductive tract (MRT) tissues was the appearance of testicular multinucleate giant cells (MGC). In rats fed either 2G or 20G, the MRT effects of 5-THG included the appearance of MGC, a lower number of germ cells at most stages of maturation, lower sperm counts and biochemical changes in testis slices and in germ cell preparations compared to rats not fed 5-THG. There were fewer Step 7 spermatids in rats fed 5-THG in 2 G than in those fed 5-THG in 20G. It is concluded that the MRT toxicity of 5-THG is influenced by diet, being potentiated by the low protein diet high in free fatty acids and, to a lesser extent, by low glucose levels within these diets.
D000704:Analysis of Variance; D000818:Animals; D001786:Blood Glucose; D001835:Body Weight / Q000187:drug effects; D004032:Diet; D005298:Fertility / Q000187:drug effects*; D005632:Fructose / Q000494:pharmacology; D005837:Genitalia, Male / Q000187:drug effects*; D005947:Glucose / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000633:toxicity; D008239:Lysine / Q000378:metabolism; D008297:Male; D009929:Organ Size / Q000187:drug effects; D051381:Rats; D013091:Spermatogenesis / Q000187:drug effects; D013739:Testosterone / Q000097:blood; D014316:Tritium
D001786:Blood Glucose; D014316:Tritium; D005632:Fructose; C003002:5-thio-D-glucose; D013739:Testosterone; D005947:Glucose; D008239:Lysine