title: Production of endothelium derived relaxant factor is dependent on oxidative phosphorylation and extracellular calcium.
abstract: The production (synthesis or release or both) of endothelium derived relaxant factor was studied in rabbit aortic strip preparations and an aortic-coronary artery bioassay system. Production of endothelium derived relaxant factor was rapidly inhibited by agents that inhibit mitochondrial electron transport or F1-ATPase, or which uncouple oxidative phosphorylation, but was only slowly impaired by inhibition of glycolysis. It was dependent also on the presence of extracellular calcium with a rapid on-off response time. This study shows that production of endothelium derived relaxant factor appears to be dependent on both oxidative phosphorylation and extracellular calcium.
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D000968:Antimycin A / Q000494:pharmacology; D001011:Aorta / Q000378:metabolism; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000502:physiology*; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology; D009131:Muscle, Smooth, Vascular / Q000378:metabolism; D009569:Nitric Oxide; D010085:Oxidative Phosphorylation; D011817:Rabbits; D012402:Rotenone / Q000494:pharmacology; D012427:Rutamycin / Q000494:pharmacology; D014634:Valinomycin / Q000494:pharmacology; D014665:Vasodilator Agents / Q000096:biosynthesis* / Q000378:metabolism
chemical_list: D014665:Vasodilator Agents; D012402:Rotenone; D014634:Valinomycin; D009569:Nitric Oxide; D000001:Calcimycin; D012427:Rutamycin; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D000968:Antimycin A; D000109:Acetylcholine; D002118:Calcium