Anomeric specificity of D-glucose metabolism in rat adipocytes.
The anomeric specificity of D-glucose metabolism was investigated in rat adipocytes exposed for 60 min at 8 degrees C to pure alpha- or beta-D-glucose or to equilibrated D-glucose. The rate of D-[5-3H]glucose utilization was higher with alpha- than beta-D-glucose. However, as judged from the oxidation of D-[1-14C]glucose and D-[6-14C]glucose anomers, the fraction of D-glucose catabolism occurring via the pentose cycle was higher with beta- than alpha-D-glucose. In the presence of equilibrated D-glucose, the utilization of alpha-D-[5-3H]glucose and the oxidation of both alpha-D-[1-14C]glucose and alpha-D-[6-14C]glucose were higher, relative to the anomer concentration, than the corresponding values for beta-D-glucose. It is concluded that the anomeric specificity of D-glucose metabolism is operative in adipocytes, even when they are exposed to equilibrated D-glucose.
D000273:Adipose Tissue / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D002245:Carbon Dioxide / Q000378:metabolism; D002250:Carbon Radioisotopes; D005947:Glucose / Q000378:metabolism*; D006019:Glycolysis; D008297:Male; D010084:Oxidation-Reduction; D051381:Rats; D013237:Stereoisomerism; D014316:Tritium
D002250:Carbon Radioisotopes; D014316:Tritium; D002245:Carbon Dioxide; D005947:Glucose