title: Induction of SOS functions by nitrogen dioxide in Escherichia coli with different DNA-repair capacities.
abstract: The effect of gaseous nitrogen dioxide (NO2) on cytotoxicity, induction of synthesis of UmuC and RecA proteins, and mutagenesis was studied in Escherichia coli strains with different capacities of DNA repair. Gaseous NO2 (90, 180 microliter/l) killed Escherichia coli. The recA mutant was most sensitive, the lexA mutant moderately sensitive, and the uvrA mutant and the wild-type the least sensitive. When 90 microliter/l NO2 gas was bubbled into bacterial suspensions for 30 min at a flow rate of 100 ml/min, the induction of umuC gene expression increased in the wild-type strain. NO2 also induced the recA gene expression in the wild-type strain. The synthesis of neither RecA nor UmuC proteins was induced in the recA and lexA mutants. We further investigated the NO2 mutagenesis in the cells treated with bubbling of NO2 gas. NO2 caused mutation to Trp+ of WP2.
mesh_terms: D004260:DNA Repair / Q000187:drug effects*; D004269:DNA, Bacterial / Q000096:biosynthesis; D004790:Enzyme Induction / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D005798:Genes, Bacterial; D009152:Mutagenicity Tests; D009153:Mutagens; D009585:Nitrogen Dioxide / Q000633:toxicity*; D001616:beta-Galactosidase / Q000096:biosynthesis
chemical_list: D004269:DNA, Bacterial; D009153:Mutagens; D001616:beta-Galactosidase; D009585:Nitrogen Dioxide