title: Comparison and critical evaluation of six published extraction and clean-up procedures for aflatoxin M1 in liquid milk.
abstract: A practical evaluation has been carried out of six previously published extraction and clean-up methods for aflatoxin M1 in liquid milk. The procedures evaluated incorporated the most widely used stages of clean-up including solvent extraction and silica gel chromatographic clean-up, selective solvent extraction of the extracted residue, the use of deproteination prior to hydrophilic column liquid-liquid partition or solvent extraction and the use of pre-packed reversed-phase cartridges for the direct extraction of aflatoxin M1 from the milk. Analysis times for each method, recoveries and relative costs are reported together with fluorescence high-performance liquid chromatography chromatograms, obtained under identical conditions to compare the relative cleanliness of the final extracts produced by each method. A pre-packed reversed phase cartridge method was shown to be the most satisfactory in terms of speed, cost and cleanliness of the final residue.
mesh_terms: D016607:Aflatoxin M1; D000348:Aflatoxins / Q000032:analysis / Q000302:isolation & purification*; D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D005069:Evaluation Studies as Topic; D005506:Food Contamination / Q000032:analysis*; D005516:Food Microbiology; D008892:Milk / Q000032:analysis*; D013050:Spectrometry, Fluorescence
chemical_list: D000348:Aflatoxins; D016607:Aflatoxin M1