Gold-resistant bacteria: excretion of a cystine-rich protein by Pseudomonas cepacia induced by an antiarthritic drug.
P. cepacia bacteria adapted to growth in a chemically defined medium containing millimolar concentrations of Au(I) thiolates including the antiarthritic drug Au(I) thiomalate. The bacteria became very large, accumulated polyhydroxybutyrate and gold, and excreted a yellow protein ("thiorin"), which caused foaming of the culture medium. Thiorin was shown by 1H-NMR, amino acid analysis, and gel filtration chromatography to be of low molecular weight (ca. 9500) and to contain predominantly Cys (oxidized), Glx, and Gly.
D001426:Bacterial Proteins / Q000378:metabolism*; D003594:Cytoplasmic Granules / Q000648:ultrastructure; D004352:Drug Resistance, Microbial; D006052:Gold Sodium Thiomalate / Q000494:pharmacology*; D006885:Hydroxybutyrates / Q000032:analysis; D008854:Microscopy, Electron; D011091:Polyesters; D011108:Polymers / Q000032:analysis; D011549:Pseudomonas / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D000094122:Polyhydroxybutyrates
D001426:Bacterial Proteins; D006052:Gold Sodium Thiomalate; D006885:Hydroxybutyrates; D011091:Polyesters; D011108:Polymers; C050983:thiorin protein, Pseudomonas; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates