Measurement of oxygen tension in the ischemic myocardium using encased polargraphic oxygen electrodes.
The ability to continuously monitor the delicate balance between blood flow and oxygen consumption would be a great asset in the study of myocardial ischemia. The present study was performed, in anesthetized dogs, to validate the use of encased polargraphic oxygen electrodes in the study of myocardial ischemia. Polargraphic oxygen electrodes were placed in the area to be rendered ischemic at fixed tissue depths of 3 mm (epicardium) and 9 mm (endocardium). Endocardial and epicardial oxygen tensions as well as the ratio of endocardial to epicardial oxygen tension and left circumflex coronary flow were monitored. Ischemia was induced by decreasing left circumflex coronary flow by 50%. Upon completion of a 20-min poststenotic period, endocardial pO2, endocardial/epicardial ratio, and coronary flow were significantly decreased (59 +/- 7, 52 +/- 7, and 55 +/- 4%, respectively) whereas epicardial pO2 was slightly decreased. Nitroglycerin (10 micrograms/kg, i.v.) markedly increased endocardial pO2 and endocardial/epicardial ratio above poststenotic control (13 +/- 5 mmHg and 64 +/- 10%, respectively) whereas epicardial pO2 was not significantly decreased. The increases in endocardial pO2 occurred at a point where coronary flow and mean arterial pressure were not significantly changed. Conversely, dipyridamole (125 micrograms/kg, i.v.) significantly increased coronary flow (26 +/- 2 ml/min/100 g) although it did not appreciably alter endocardial or epicardial pO2. It is concluded that encased polargraphic oxygen electrodes provide a quantitative method for determination of oxygen tension in the ischemic myocardium.
D000818:Animals; D003327:Coronary Disease / Q000188:drug therapy / Q000378:metabolism*; D004176:Dipyridamole / Q000627:therapeutic use; D004285:Dogs; D004566:Electrodes; D005260:Female; D008297:Male; D009206:Myocardium / Q000032:analysis*; D005996:Nitroglycerin / Q000627:therapeutic use; D010100:Oxygen / Q000032:analysis*; D010600:Pharmacology / Q000295:instrumentation*; D011048:Polarography / Q000295:instrumentation*
D004176:Dipyridamole; D005996:Nitroglycerin; D010100:Oxygen