Leptomonas samueli glycoconjugates. Comparison with Herpetomonas samuelpessoai.
Xylose and mannose are the main manosaccharide components of Herpetomonas samuelpessoai and Leptomonas samueli promastigotes. Variations in the xylose/mannose ratios are related to the age of cultures. Phenol-aqueous extraction disclosed in both species the presence of carbohydrate-containing fractions which were both soluble and insoluble in chloroform/methanol/water (10:10.3). The xylose enriched, uronic acid-containing glycoconjugates of L. samueli were mainly composed of (1----4)-linked xylose units (Methylation-mass spectrometry and 13C NMR), similar to the glucuronoxylan of H. samuelpessoai (Mendonça-Previato et al., 1979 Biochem 18 149-154). SDS-PAGE and sugar composition analysis disclosed similarities between glycoconjugates of H. samuelpessoai and L. samueli, two species dwelling in the same host, therefore an example of convergent adaptation.
D000818:Animals; D002241:Carbohydrates / Q000032:analysis*; D008358:Mannose / Q000032:analysis; D008745:Methylation; D011134:Polysaccharides / Q000032:analysis*; D013045:Species Specificity; D014351:Trypanosomatina / Q000032:analysis*; D014994:Xylose / Q000032:analysis
D002241:Carbohydrates; D011134:Polysaccharides; D014994:Xylose; D008358:Mannose