title: Genetic and physiological characterization of Bacillus subtilis mutants resistant to purine analogs.
abstract: Bacillus subtilis mutants defective in purine metabolism have been isolated by selecting for resistance to purine analogs. Mutants resistant to 2-fluoroadenine were found to be defective in adenine phosphoribosyltransferase (apt) activity and slightly impaired in adenine uptake. By making use of apt mutants and mutants defective in adenosine phosphorylase activity, it was shown that adenine deamination is an essential step in the conversion of both adenine and adenosine to guanine nucleotides. Mutants resistant to 8-azaguanine, pbuG mutants, appeared to be defective in hypoxanthine and guanine transport and normal in hypoxanthine-guanine phosphoribosyltransferase activity. Purine auxotrophic pbuG mutants grew in a concentration-dependent way on hypoxanthine, while normal growth was observed on inosine as the purine source. Inosine was taken up by a different transport system and utilized after conversion to hypoxanthine. Two mutants resistant to 8-azaxanthine were isolated: one was defective in xanthine phosphoribosyltransferase (xpt) activity and xanthine transport, and another had reduced GMP synthetase activity. The results obtained with the various mutants provide evidence for the existence of specific purine base transport systems. The genetic lesions causing the mutant phenotypes, apt, pbuG, and xpt, have been located on the B. subtilis linkage map at 243, 55, and 198 degrees, respectively.
mesh_terms: D000225:Adenine / Q000031:analogs & derivatives / Q000494:pharmacology; D000228:Adenine Phosphoribosyltransferase / Q000235:genetics / Q000378:metabolism; D001375:Azaguanine / Q000494:pharmacology; D001412:Bacillus subtilis / Q000187:drug effects / Q000235:genetics*; D001692:Biological Transport; D002874:Chromosome Mapping; D004352:Drug Resistance, Microbial; D005798:Genes, Bacterial; D005838:Genotype; D010430:Pentosyltransferases / Q000235:genetics / Q000378:metabolism; D011687:Purines / Q000378:metabolism* / Q000494:pharmacology; D014970:Xanthines / Q000494:pharmacology
chemical_list: D011687:Purines; D014970:Xanthines; C018221:8-azaxanthine; C038208:2-fluoroadenine; D010430:Pentosyltransferases; C022484:purine phosphoribosyltransferase; C024827:xanthine phosphoribosyltransferase; D000228:Adenine Phosphoribosyltransferase; D000225:Adenine; D001375:Azaguanine