The indirect flight muscle of Drosophila accumulates a unique myosin alkali light chain isoform.
Mapping of the 5' and 3' ends of the Drosophila myosin alkali light chain (MLC-ALK) mRNA by S1 nuclease and primer extension assays has shown that the primary transcripts are identical irrespective of the time in development that the RNA was prepared. As shown by S1 nuclease experiments these transcripts are alternatively spliced in a tissue-specific fashion generating mRNAs that encode tissue-specific protein isoforms. Antibodies were raised to synthetic peptides identical in sequence to the unique portion of each protein. Western blots of one-dimensional polyacrylamide gels using the type-specific antibodies confirmed and extended the results obtained from the S1 nuclease experiments. The indirect flight muscle is the only tissue in the adult that accumulates the alternatively spliced mRNA. The choice between splicing pathways involves the use of a nonconsensus 3' splice junction in larvae and in the tubular muscles of adults, whereas in the indirect flight muscle of the adult only consensus sequences are utilized. The involvement of a trans-acting factor to activate the nonconsensus splice site in the myotubes of larvae and the tubular myotubes of adults is proposed.
D000818:Animals; D002454:Cell Differentiation; D004331:Drosophila melanogaster / Q000235:genetics* / Q000254:growth & development; D005786:Gene Expression Regulation; D007814:Larva; D009132:Muscles / Q000166:cytology / Q000502:physiology; D009218:Myosins / Q000235:genetics*; D011061:Poly A / Q000235:genetics; D011401:Promoter Regions, Genetic; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics; D014018:Tissue Distribution
D012333:RNA, Messenger; D011061:Poly A; D009218:Myosins