Effect of interleukin-1 on intracellular concentration of sodium, calcium, and potassium in 70Z/3 cells.
The cytokine interleukin-1 (IL-1) has been shown to induce the differentiation of a murine pre-B like cell line 70Z/3. An early intracellular event caused by exposure to IL-1 is an amiloride-sensitive progressive rise in the total concentration of intracellular sodium ([Na]i), caused by influx of Na+ from outside, and a transient fall in total intracellular calcium. The results suggest that IL-1-induced influx of Na+ into 70Z/3 occurs via stimulation of the Na+/H+ antiport. IL-1-induced differentiation is also blocked by amiloride suggesting that stimulation of Na+/H+ exchange may play a role in IL-1-induced differentiation.
D000584:Amiloride / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002460:Cell Line; D005434:Flow Cytometry; D007371:Interferon-gamma / Q000494:pharmacology; D007375:Interleukin-1 / Q000494:pharmacology*; D007700:Kinetics; D051379:Mice; D009374:Neoplasms, Experimental; D011188:Potassium / Q000378:metabolism*; D011947:Receptors, Antigen, B-Cell / Q000032:analysis; D012964:Sodium / Q000378:metabolism*
D007375:Interleukin-1; D011947:Receptors, Antigen, B-Cell; D000584:Amiloride; D007371:Interferon-gamma; D012964:Sodium; D011188:Potassium; D002118:Calcium