Improved preservation of glycogen in unfixed cyanobacteria embedded at -82 degrees C in nanoplast.
A technique is presented for embedding unfixed cells at -82 degrees C in Nanoplast, a water-soluble melamine resin. By use of this procedure, improved ultrastructural preservation is demonstrated in cyanobacteria. Sections treated in this way are suitable for histochemical techniques such as Thiéry's silver staining method for glycogen.
D003080:Cold Temperature; D003451:Cryoprotective Agents; D000458:Cyanobacteria / Q000378:metabolism* / Q000648:ultrastructure; D006003:Glycogen / Q000032:analysis*; D006652:Histological Techniques; D008854:Microscopy, Electron; D011309:Preservation, Biological / Q000592:standards*; D012117:Resins, Synthetic; D014227:Triazines
D003451:Cryoprotective Agents; D012117:Resins, Synthetic; D014227:Triazines; C050897:nanoplast; D006003:Glycogen