Measurement of arachidonate and its metabolites extracted from human normal and malignant gastrointestinal tissues.
This is the first report of human gastrointestinal arachidonate and prostanoids measured quantitatively by gas chromatography-mass spectrometry (GC-MS) in extracts of human cancers and macroscopically normal tissues from the stomach and colon. There were microgram/g amounts of arachidonate, and the particularly high yield from the tumours may explain why they usually produce more prostaglandins than the normal tissues in which they arise. There was only a small conversion of the arachidonate into prostanoids. 6-Keto-PGF1 alpha was the most abundant metabolite measured, particularly in the tumour extracts, with smaller amounts of prostaglandins E2, F2 alpha and D2.
D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000032:analysis / Q000378:metabolism*; D003106:Colon / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry; D005770:Gastrointestinal Neoplasms / Q000032:analysis*; D006801:Humans; D011453:Prostaglandins / Q000032:analysis*; D013270:Stomach / Q000032:analysis
D001095:Arachidonic Acids; D011453:Prostaglandins; D016718:Arachidonic Acid