A comparison of two fluorescence-activated cell sorters, the FACSIV (laser) and the FACSTm (mercury lamp), as research analyzers for the quantification of T and B cell subsets in human peripheral blood.
T cell subset determinations were performed on 146 peripheral blood samples from healthy volunteers, and on 112 samples from immune deficient patients using two fluorescence-activated cell sorters (the FACSIV laser, and the FACSTm mercury lamp analyzer). The procedures necessary for the use and calibration of the FACSTm analyzer are discussed, and detailed. Using the FACSTm analyzer, counts were made of T and B cell subsets in 28 patients with multiple infections, 9 patients suffering from the acquired immune deficiency syndrome (AIDS) and 16 patients with a primary immunodeficiency disease. These results were compared with data obtained from 47 healthy volunteers, as control references. Results from the two instruments proved closely comparable, both qualitatively and quantitatively.
D001402:B-Lymphocytes / Q000145:classification*; D002469:Cell Separation; D005434:Flow Cytometry; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D006801:Humans; D007834:Lasers; D008628:Mercury; D013601:T-Lymphocytes / Q000145:classification*; D013861:Thiocyanates
D005452:Fluoresceins; D013861:Thiocyanates; D008628:Mercury; D016650:Fluorescein-5-isothiocyanate