Bactericidal activity of the membrane fraction isolated from phagocytes of mice and its stimulation by melittin.
The membrane fraction prepared from mouse peritoneal exudate cells was incubated with mycobacteria, staphylococci, or E. coli in acetate buffer of pH 5.6 to follow the fate of viable bacilli. The membrane fraction exhibited bactericidal effect on mycobacteria and staphylococci, but not on E. coli. The activity to kill mycobacteria, as well as the endogenous phospholipase A2 activity, of the membrane fraction was markedly enhanced by melittin, a basic peptide from bee venom, and inhibited by indomethacin and EDTA. The role of the enzyme activity in the bactericidal activity was discussed.
D000818:Animals; D001514:Bee Venoms / Q000494:pharmacology*; D002462:Cell Membrane / Q000502:physiology; D004492:Edetic Acid / Q000494:pharmacology; D004926:Escherichia coli / Q000502:physiology*; D007213:Indomethacin / Q000494:pharmacology; D008297:Male; D008555:Melitten / Q000494:pharmacology*; D051379:Mice; D009161:Mycobacterium / Q000502:physiology*; D009163:Mycobacterium bovis / Q000187:drug effects; D009169:Mycobacterium tuberculosis / Q000187:drug effects; D009504:Neutrophils / Q000502:physiology / Q000648:ultrastructure; D010586:Phagocytes / Q000201:enzymology / Q000502:physiology* / Q000648:ultrastructure; D010741:Phospholipases A / Q000378:metabolism; D054467:Phospholipases A2; D013211:Staphylococcus aureus / Q000502:physiology*
D001514:Bee Venoms; D008555:Melitten; D004492:Edetic Acid; D010741:Phospholipases A; D054467:Phospholipases A2; D007213:Indomethacin