[Characteristics of 3,4-benzopyrene-induced cytochrome P-448 forms isolated from mouse liver microsomes].
Two cytochrome P-448 fractions, B1 and B2, were isolated from liver microsomes of 3,4-benzpyrene-induced inbred C57Bl/6 mice, using chromatography on octyl-Sepharose CL-4B and on Whatman 52E. During subsequent chromatography on hydroxylapatite fraction B1 was separated into 2 subfractions, G1 and G2. Cytochrome fractions B1, G1 and G2 have similar "peptide maps" differing from that of fraction B2. Cytochrome fraction B1 is immunologically identical to G2, partly to fraction B2 but is distinct from fraction G1. Fraction G2 is identified as the form of cytochrome P-448 catalyzing the hydroxylation of 3,4-benzpyrene and 7-ethoxyresorufin and existing in a low spin form. Cytochrome fraction G1 is apparently identical to the form P3-450. Fraction B2 was not yet described in current literature, since cytochrome P-448 (Mr = 53,000 Da) was identified only after the induction of mice with 3,4-benzpyrene but not with other inducers, e.g., polycyclic aromatic hydrocarbons.
D000818:Animals; D001564:Benzo(a)pyrene / Q000494:pharmacology*; D002852:Chromatography, Ion Exchange; D019388:Cytochrome P-450 CYP1A2; D003580:Cytochromes / Q000096:biosynthesis* / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D004790:Enzyme Induction; D005779:Immunodiffusion; D007527:Isoenzymes / Q000096:biosynthesis / Q000302:isolation & purification; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008862:Microsomes, Liver / Q000201:enzymology*
D003580:Cytochromes; D007527:Isoenzymes; D001564:Benzo(a)pyrene; C017170:Cyp1a2 protein, rat; D019388:Cytochrome P-450 CYP1A2
Kharakteristika indutsirovannykh 3,4-benzpirenom form tsitokhroma P-448, izolirovannykh iz mikrosom pecheni mysheĭ.