Cytochemistry of N-acetyl-beta-glucosaminidase in normal and leukemic T cells.
A cytochemical study of the N-acetyl-beta-glucosaminidase (NABG) activity in normal and leukemic T cells was carried out to ascertain any relationships between cytochemical reactivity and membrane phenotype. Under normal conditions, most T4-positive cells, defined on the basis of monoclonal antibodies and immunogold, were characterized by focal reactivity. In chronic T lymphoproliferative disorders, the reaction product of T4-positive cells consisted of a single coarse granule, while T8-positive cells demonstrated many scattered granules. In all cases of acute T cell malignancies, lymphoblasts were positive with a single coarse granule or many coarse and small granules located at one pole of the cell.
D000118:Acetylglucosaminidase / Q000378:metabolism*; D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface / Q000032:analysis; D002648:Child; D002908:Chronic Disease; D005260:Female; D006596:Hexosaminidases / Q000378:metabolism*; D006651:Histocytochemistry; D006801:Humans; D007938:Leukemia / Q000097:blood / Q000201:enzymology* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D013601:T-Lymphocytes / Q000145:classification / Q000201:enzymology*
D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface; D006596:Hexosaminidases; D000118:Acetylglucosaminidase