Comparison of methods for isolation and titration of Crimean-Congo hemorrhagic fever virus.
The fluorescence focus assay and the plaque assay in CER cells were compared with mouse inoculation for the isolation and titration of Crimean-Congo hemorrhagic fever virus. The fluorescence focus assay and the plaque assay were of similar sensitivity, but both produced 10- to 100-fold lower titers than did mouse inoculation. For specimens from 26 Crimean-Congo hemorrhagic fever patients in South Africa, virus was isolated from 20 by mouse inoculation and from only 11 by cell culturing. Although cell cultures were less sensitive for the isolation of virus from clinical specimens, they produced diagnostic results much more rapidly.
D000818:Animals; D002043:Bunyaviridae / Q000302:isolation & purification*; D002460:Cell Line; D005455:Fluorescent Antibody Technique; D003225:Hemorrhagic Fever Virus, Crimean-Congo / Q000254:growth & development / Q000302:isolation & purification*; D006479:Hemorrhagic Fever, Crimean / Q000175:diagnosis*; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D014709:Vero Cells; D010948:Viral Plaque Assay