An automated method for the determination of Cu,Zn-superoxide dismutase in plasma and erythrocytes using an ABA-200 discrete analyzer.
An automated enzymatic method is described for the determination of Cu,Zn-superoxide dismutase (SOD) in plasma or erythrocytes using the xanthine-xanthine oxidase and cytochrome C coupled assay. This method was adapted to an Abbott ABA-200 discrete analyzer. Coefficients of variation for within-run and day-to-day analyses were less than 5%. Only 2.5 muL of serum or erythrocyte extract is required so a capillary tube sample of blood (70 muL) is sufficient for the assay. Recovery of added SOD ranged from 92 to 101%. The method reported here is practical for use in a clinical chemistry laboratory for monitoring changes in this enzyme, which is a sensitive early indicator of alterations in copper status.
D000818:Animals; D001322:Autoanalysis / Q000379:methods / Q000592:standards; D004796:Clinical Enzyme Tests / Q000379:methods* / Q000592:standards; D004912:Erythrocytes / Q000201:enzymology*; D005260:Female; D000882:Haplorhini; D006801:Humans; D008297:Male; D010949:Plasma / Q000201:enzymology*; D051381:Rats; D013482:Superoxide Dismutase / Q000097:blood* / Q000592:standards
D013482:Superoxide Dismutase