Structure of a new disialoganglioside GD1c from spontaneous murine thymoma.
A major mono- and a di-sialoganglioside were isolated and purified to homogeneity from a spontaneous thymoma that occurs in AKR mice. Compositional and methylation analyses and the use of exoglycosidases established the monosialoganglioside to be alpha Neu(2----3)beta Gal(1----3)beta GalNAc(1----4)beta Gal(1----4)Glc(1----4)Cer and the disialoganglioside to be alpha NeuAc(2----8)alpha NeuAc(2----3)beta Gal(1----3)beta GalNAc(1----4)beta Gal(1----4)Glc(1----1)Cer (GD1c). A possible pathway for the biosynthesis of this disialoganglioside is presented.
D000818:Animals; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002855:Chromatography, Thin Layer; D005227:Fatty Acids / Q000032:analysis; D005732:Gangliosides / Q000302:isolation & purification*; D008401:Gas Chromatography-Mass Spectrometry; D006026:Glycoside Hydrolases; D008745:Methylation; D051379:Mice; D008806:Mice, Inbred AKR; D013945:Thymoma / Q000032:analysis*; D013953:Thymus Neoplasms / Q000032:analysis*
D005227:Fatty Acids; D005732:Gangliosides; D006026:Glycoside Hydrolases