Modulation of phorbol ester receptors in mouse skin by application of quercetin.
The topical application of quercetin, an anti-tumor promoter, to mouse skin reduced the number of phorbol ester receptors, although quercetin did not inhibit specific 3H-12-O-tetradecanoylphorbol-13-acetate binding to a mouse skin particulate fraction. Quercetin, morin, kaempferol and luteolin inhibited activation of protein kinase C by teleocidin, and caused half-maximal activation at 25 microM. (+)-Catechin, which has been reported not to inhibit tumor-promoting activity, did not have any effect on these reactions. The modulation of phorbol ester receptors and inhibition of activation of protein kinase C are considered to be involved in the anti-tumor-promoting effect of quercetin in mouse skin. Diet containing 4% or 1% quercetin did not influence the action of teleocidin on mouse skin in a two-stage carcinogenesis experiment.
D000818:Animals; D029742:Caenorhabditis elegans Proteins; D002352:Carrier Proteins; D005419:Flavonoids / Q000494:pharmacology*; D008235:Lyngbya Toxins / Q000494:pharmacology; D051379:Mice; D008815:Mice, Inbred Strains; D011493:Protein Kinase C / Q000032:analysis; D011794:Quercetin / Q000378:metabolism / Q000494:pharmacology*; D011955:Receptors, Drug; D011971:Receptors, Immunologic / Q000187:drug effects*; D012867:Skin / Q000032:analysis / Q000187:drug effects*; D012878:Skin Neoplasms / Q000139:chemically induced / Q000517:prevention & control; D013755:Tetradecanoylphorbol Acetate / Q000378:metabolism; D014316:Tritium
D029742:Caenorhabditis elegans Proteins; D002352:Carrier Proteins; D005419:Flavonoids; D008235:Lyngbya Toxins; D011955:Receptors, Drug; D011971:Receptors, Immunologic; C083141:phorbol ester binding protein; C027776:phorbol ester receptor; D014316:Tritium; C022176:teleocidins; D011794:Quercetin; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate