title: Lectin binding to newt epidermis: fluorescent localization and effects on motility.
abstract: The ability of seven lectins to bind to newt epidermal cells and influence their motility was examined. Of the seven fluoresceinated lectins applied to frozen sections containing intact newt skin and migrating epidermis (wound epithelium), only Con A (concanavalin A), WGA (wheat germ agglutinin), and PNA (peanut agglutinin) produced detectable epidermal fluorescence. Con A and WGA each heavily labeled all layers of intact epidermis, but PNA bound only to the more superficial layers. In contrast to a single population of labeled cells in migrating epidermal sheets after treatment with Con A, there were both labeled and unlabeled cells after exposure to either WGA or PNA. The wound bed was labeled by both Con A and WGA, but not by PNA. DBA (Dolichos bifloris agglutinin), RCA I (Ricinus communis agglutinin), and UEA (Ulex europaeus agglutinin), did not produce significant fluorescence with either migrating or intact epidermis. In general, inhibitory effects on epidermal motility correlated with the binding studies. Thus, Con A, WGA, and PNA, the lectins which clearly bound to the epidermis, all produced a concentration-dependent depression in the rate of epidermal wound closure. RCA was somewhat paradoxical in that it was moderately inhibitory despite showing essentially no binding. The effects of SBA and UEA were equivocal. DBA had no effect. These results indicate that the inhibition of motility produced by Con A that we have described previously is not peculiar to this mannose-binding lectin, but is shared by at least one lectin with an affinity for D-GlcNAc (WGA), and one with an affinity for B-D-Gal(1-3)-D-GalNAc (PNA).(ABSTRACT TRUNCATED AT 250 WORDS)
mesh_terms: D000818:Animals; D002465:Cell Movement; D004817:Epidermis / Q000276:immunology / Q000502:physiology; D004847:Epithelial Cells; D004848:Epithelium / Q000276:immunology / Q000502:physiology; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D037102:Lectins / Q000378:metabolism*; D011975:Receptors, Mitogen / Q000378:metabolism*; D012455:Salamandridae; D012867:Skin / Q000276:immunology; D012879:Skin Physiological Phenomena; D013050:Spectrometry, Fluorescence; D013329:Structure-Activity Relationship; D013861:Thiocyanates
chemical_list: D005452:Fluoresceins; D037102:Lectins; D011975:Receptors, Mitogen; D013861:Thiocyanates; D016650:Fluorescein-5-isothiocyanate