Mechanisms of platelet aggregation by viridans group streptococci.
The direct aggregation of platelets is thought to be an important event in the pathogenesis of viridans streptococcal endocarditis, but the mechanisms for platelet activation are unknown. We evaluated the processes by which two endocarditis-producing strains of viridans group streptococci activated human platelets in vitro, as measured by platelet cyclooxygenase activity, secretion, and aggregation. Addition of either streptococcal strain to platelets suspended in whole plasma resulted in a mean lag phase of 15.3 min, followed by platelet secretion and brisk aggregation. The lag phase duration was dependent on the platelet donor and appeared to be a function of direct platelet-bacterial interaction. Aggregation was partially inhibited by 20 muM [corrected] indomethacin and blocked completely by 1 mg of apyrase, an extracellular ADP hydrolase, per ml. Neither strain aggregated washed platelets suspended in Tyrode solution alone. However, both strains produced maximal aggregation when the platelet suspension was supplemented with 10% (final concentration) normal plasma. Studies with factor-deficient plasmas demonstrated that exogenous fibrinogen was required for aggregation. One or more additional plasma components were needed, which eluted with a molecular weight of 67,000 to 130,000 on gel permeation chromatography. These cofactors have not been described for other platelet agonists, which suggests that viridans streptococci may aggregate human platelets by a novel mechanism.
D000244:Adenosine Diphosphate / Q000097:blood; D001792:Blood Platelets / Q000502:physiology*; D004697:Endocarditis, Bacterial / Q000503:physiopathology*; D005340:Fibrinogen / Q000502:physiology; D006801:Humans; D010974:Platelet Aggregation; D011451:Prostaglandin-Endoperoxide Synthases / Q000097:blood; D013291:Streptococcus / Q000472:pathogenicity*; D013298:Streptococcus sanguis / Q000472:pathogenicity*; D013997:Time Factors
D000244:Adenosine Diphosphate; D005340:Fibrinogen; D011451:Prostaglandin-Endoperoxide Synthases