Increase in factor VIII clotting activity in the perfusate of isolated dog hind leg and heart by components of kallikrein-kinin system.
Transient increase in Factor VIII clotting (F.VIIIc) activity was observed by infusion of kallikrein or bradykinin (BK) to the isolated dog leg or heart preparation which was perfused with physiological solution, but the increase in Factor IX activity was not observed, suggesting that the increase in F.VIIIc activity was not due to contaminated plasma components of the perfusate. The increase coincided with the increase in prostacyclin (PGI2) which was determined by immunoassay of 6-keto-PGF1 alpha and by platelet aggregation inhibiting activity. Although increase in PGI2 by BK was inhibited by the pretreatment of the preparations with indomethacin or tranylcypromine, the increase in F.VIIIc activity was not inhibited, suggesting that the increase in F.VIIIc activity was not mediated by PGI2.
D000818:Animals; D001777:Blood Coagulation / Q000187:drug effects; D001920:Bradykinin / Q000494:pharmacology; D004285:Dogs; D011464:Epoprostenol / Q000097:blood; D005169:Factor VIII / Q000502:physiology*; D006321:Heart / Q000187:drug effects / Q000502:physiology*; D006614:Hindlimb / Q000098:blood supply; D007610:Kallikreins / Q000494:pharmacology / Q000502:physiology*; D007705:Kinins / Q000502:physiology*; D010974:Platelet Aggregation
D007705:Kinins; D005169:Factor VIII; D011464:Epoprostenol; D007610:Kallikreins; D001920:Bradykinin