Effects of pretreatment with 2,3,7,8-tetrachlorodibenzofuran on microsomal monooxygenase activity in Drosophila melanogaster.
The effects of tetrachlorodibenzofuran pretreatment was studied in microsomes from adult Drosophila of the Karsnäs 60w strain. Pretreatment for 18 h with 0.2 or 1 mg TCDBF did not increase benzo[alpha]pyrene monooxygenase activity, 7-ethyoxyresorufin deethylase activity or the formation of 2-, 3- or 4-hydroxylated metabolites from biphenyl, in accordance with previous results with alpha-naphthoflavone pretreatment. The results suggest that adult Drosophila is non-responsive to induction by polycyclic aromatic hydrocarbons. The role of the TCDD receptor, which has been reported to be present in Drosophila, is obscure against this background. The implications for mutagenicity testing in Drosophila is discussed.
D000818:Animals; D001572:Benzofurans / Q000494:pharmacology*; D002458:Cell Fractionation; D004331:Drosophila melanogaster / Q000187:drug effects / Q000201:enzymology*; D004785:Environmental Pollutants; D007700:Kinetics; D008861:Microsomes / Q000187:drug effects / Q000201:enzymology* / Q000648:ultrastructure; D006899:Mixed Function Oxygenases / Q000096:biosynthesis*
D001572:Benzofurans; D004785:Environmental Pollutants; D006899:Mixed Function Oxygenases; C014211:2,3,7,8-tetrachlorodibenzofuran