Sensitivity to DNA-damaging agents and mutation induction by UV light in UV-sensitive CHO cells.
Three UV-sensitive (UVs) mutants isolated from a CHO cell line were analyzed for survival after exposure to H2O2, EMS, MMC, CCNU, X-rays and for mutation induction after UV-irradiation. The UVs mutants showed normal sensitivities to EMS and H2O2, whereas they were hypersensitive to the bifunctional alkylating agents MMC and CCNU and to hypoxic X-irradiation. Compared to parental cells, one of the UV-sensitive clones showed approximately 3- and 7-fold enhancement in the mutagenic response per unit UV dose for 6-thioguanine and ouabain resistance, respectively.
D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D006224:Cricetinae; D003412:Cricetulus; D005020:Ethyl Methanesulfonate / Q000633:toxicity*; D005260:Female; D006861:Hydrogen Peroxide / Q000633:toxicity*; D008130:Lomustine / Q000633:toxicity*; D016685:Mitomycin; D008937:Mitomycins / Q000633:toxicity*; D009154:Mutation; D010053:Ovary; D014466:Ultraviolet Rays; D014965:X-Rays
D008937:Mitomycins; D016685:Mitomycin; D008130:Lomustine; D005020:Ethyl Methanesulfonate; D006861:Hydrogen Peroxide