Preliminary characterization of cell surface-extracellular matrix linkage complexes in cultured retinal pigmented epithelial cells.
In this report, we describe the relative distribution of vinculin, talin, and fibronectin in cultured retinal pigmented epithelial cells from chick embryo eyes. We show that in these cells vinculin is present in both focal cell-substratum and cell-cell contacts, whereas talin is present only in the cell-substratum contacts. When cells are double-labeled for talin and fibronectin and viewed at the substratum level, fibronectin is not detectable and talin is concentrated in plaques corresponding to focal contacts. However, when the same cells are viewed at the apical level, both talin and fibronectin are present in a fibrillar pattern. In addition to fibrils which are both talin- and fibronectin-positive, there are areas which are either talin-positive and fibronectin-negative or, vice versa, talin-negative and fibronectin-positive. These observations indicate an interesting variability in the composition of transmembrane linkages in retinal pigmented epithelial cells in vitro.
D000818:Animals; D002448:Cell Adhesion; D002462:Cell Membrane / Q000032:analysis / Q000648:ultrastructure; D002642:Chick Embryo; D003598:Cytoskeletal Proteins / Q000032:analysis*; D005109:Extracellular Matrix / Q000032:analysis* / Q000648:ultrastructure; D005353:Fibronectins / Q000032:analysis*; D007365:Intercellular Junctions / Q000032:analysis / Q000648:ultrastructure; D008857:Microscopy, Interference; D009124:Muscle Proteins / Q000032:analysis*; D010857:Pigment Epithelium of Eye / Q000032:analysis / Q000648:ultrastructure*; D016608:Talin; D016596:Vinculin
D003598:Cytoskeletal Proteins; D005353:Fibronectins; D009124:Muscle Proteins; D016608:Talin; D016596:Vinculin