Identification and characterization of the tyrosine protein kinases of rat spleen.
High levels of tyrosine protein kinase have been recently detected in the membranes of rat spleen. In the present report the tyrosine protein kinase activity of the 30,000 x g pellet of rat spleen has been solubilized and partially purified by ion exchange and gel permeation chromatography. Two peaks of tyrosine protein kinase of Mr 35,000 (TPK-I) and Mr 40,000 (TPK-II) have been resolved. These kinases were free of the EGF receptor and insulin receptor tyrosine protein kinases. Although TPK-I and TPK-II phosphorylated angiotensin II, casein, histone, tubulin, phosphorylase b, and p36 they differed from each other in preference for the substrates. Both tyrosine protein kinases did not phosphorylate anti-pp60v-src IgG.
D000804:Angiotensin II / Q000378:metabolism; D000818:Animals; D002364:Caseins / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D066246:ErbB Receptors / Q000378:metabolism; D006657:Histones / Q000378:metabolism; D007527:Isoenzymes / Q000378:metabolism; D008970:Molecular Weight; D015688:Oncogene Protein pp60(v-src); D010763:Phosphorylase b / Q000378:metabolism; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D051381:Rats; D012191:Retroviridae Proteins / Q000378:metabolism; D013154:Spleen / Q000201:enzymology*; D013379:Substrate Specificity; D014404:Tubulin / Q000378:metabolism
D002364:Caseins; D006657:Histones; D007527:Isoenzymes; D012191:Retroviridae Proteins; D014404:Tubulin; D000804:Angiotensin II; D010763:Phosphorylase b; D066246:ErbB Receptors; D011505:Protein-Tyrosine Kinases; D015688:Oncogene Protein pp60(v-src)