Cloning of a Drosophila cDNA encoding a polypeptide similar to the human insulin receptor precursor.
A Drosophila cDNA clone was obtained using the human insulin receptor cDNA sequence as a probe. The 3586 bp nucleotide sequence predicted a single polypeptide of 1095 amino acid residues which showed considerable homology (35.2%) with the human insulin receptor precursor. Although the cDNA was incomplete at its 5'-terminal region, it encodes a transmembrane glycoprotein as a single precursor of a two subunit molecule having a structural architecture similar to that of the human insulin receptor precursor. The presumptive beta subunit carries a well conserved Tyr kinase domain which showed 63.5% homology with that of human insulin receptor; however the protein of the alpha subunit is only weakly conserved (25%).
D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004331:Drosophila melanogaster / Q000235:genetics*; D006023:Glycoproteins / Q000235:genetics; D011487:Protein Conformation; D011498:Protein Precursors / Q000235:genetics; D011972:Receptor, Insulin / Q000235:genetics*; D012995:Solubility
D006023:Glycoproteins; D011498:Protein Precursors; D004247:DNA; D011972:Receptor, Insulin