title: Isolation and the fluorometric, high-performance liquid chromatographic determination of tacrine.
abstract: Tacrine (THA; 1,2,3,4-tetrahydro-9-aminoacridine) is an anticholinesterase agent which has been used clinically, most recently in the treatment of Alzheimer-type dementias. This paper describes the methodology for the isolation and quantitation of THA at therapeutic levels in serum from human subjects. Using C18 Bond Elut columns and an HPLC/fluorometry system, this assay exhibits a considerable improvement in sensitivity over previous uv methods, and allows routine testing of THA levels in serum samples of reasonable volume from human subjects.
mesh_terms: D000609:Aminoacridines / Q000097:blood*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D000882:Haplorhini; D006801:Humans; D008991:Monitoring, Physiologic; D013050:Spectrometry, Fluorescence; D013619:Tacrine / Q000097:blood* / Q000302:isolation & purification
chemical_list: D000609:Aminoacridines; D013619:Tacrine