ADP-ribosylation of specific membrane proteins in pheochromocytoma and primary-cultured brain cells by botulinum neurotoxins type C and D.
Type C1 and D toxins produced by Clostridium botulinum caused ADP-ribosylation of a protein of 24 kDa in membrane preparations of rat clonal pheochromocytoma cells (PC12) and of proteins of 25 and 26 kDa in neuron-rich culture of fetal rat brain cells. The ADP-ribosylation reaction was dependent on the presence of MgCl2, GTP and GTP gamma S. The results obtained suggested that the ADP-ribosylation reaction is responsible for the development of the biological activity of the botulinum neurotoxins and that the target of this reaction may be novel GTP-binding proteins localized on cell membranes.
D000246:Adenosine Diphosphate Ribose / Q000378:metabolism*; D001905:Botulinum Toxins / Q000494:pharmacology*; D001921:Brain / Q000378:metabolism; D002478:Cells, Cultured; D003014:Clostridium botulinum; D019204:GTP-Binding Proteins / Q000378:metabolism; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D006160:Guanosine Triphosphate / Q000031:analogs & derivatives / Q000378:metabolism; D008274:Magnesium / Q000378:metabolism; D015636:Magnesium Chloride; D008565:Membrane Proteins / Q000378:metabolism*; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009498:Neurotoxins / Q000494:pharmacology*; D010673:Pheochromocytoma; D013873:Thionucleotides / Q000378:metabolism
D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D009498:Neurotoxins; D013873:Thionucleotides; D015636:Magnesium Chloride; D000246:Adenosine Diphosphate Ribose; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D006160:Guanosine Triphosphate; D001905:Botulinum Toxins; D019204:GTP-Binding Proteins; D008274:Magnesium