Expression of cloned monkey metallothionein in Escherichia coli.
Expression vectors were constructed in which a cDNA specifying the monkey kidney metallothionein-II (MT-II) was linked directly to the lambda PR promoter. Enhanced expression of MT-II in Escherichia coli was observed when two initiation signals were tandemly linked to the MT-II gene and the lambda cI+ host cells were induced by nalidixic acid.
D000818:Animals; D003062:Codon / Q000235:genetics; D004247:DNA / Q000235:genetics; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000235:genetics*; D005786:Gene Expression Regulation; D005798:Genes, Bacterial; D005822:Genetic Vectors; D000882:Haplorhini; D008668:Metallothionein / Q000096:biosynthesis / Q000235:genetics*; D010957:Plasmids; D011401:Promoter Regions, Genetic
D003062:Codon; D004274:DNA, Recombinant; D004247:DNA; D008668:Metallothionein