Preparation of anti-bovine and porcine mu chain sera.
Absorption of anti-L chain antibodies contained in anti-IgM sera immunized with purified bovine and porcine IgM was undertaken by affinity chromatography on a column of Sepharose 4B, coupled with the normal follicular fluids of the respective species as an immunosorbent. Anti-L chain antibodies in both anti-IgM sera were completely absorbed without incurring reduction of the antibody titer, and anti-bovine and anti-porcine mu chain sera were prepared.
D000818:Animals; D002417:Cattle; D006041:Goats / Q000276:immunology; D007106:Immune Sera; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D007075:Immunoglobulin M / Q000276:immunology; D007148:Immunoglobulin mu-Chains / Q000302:isolation & purification*; D013552:Swine
D007106:Immune Sera; D007075:Immunoglobulin M; D007148:Immunoglobulin mu-Chains