Effects of mast cell-derived mediators on epithelial cells in canine trachea.
We examined the interaction between mast cell-derived mediators and the electrical and ion transport properties of canine tracheal epithelium. We compared the effect of mediators released by immunologic challenge of sensitized lung parenchyma with that of mediators released from canine mastocytoma cells challenged with calcium ionophore A23187. Short-circuit current (Isc) increased by 19.2 +/- 3.0 microA/cm2 in response to mediators released from sensitized lung fragments challenged with ragweed antigen. This effect was not due to histamine. When the epithelial tissues were pretreated with indomethacin, the same mediator supernatant increased Isc by only 3.8 +/- 4.3 microA/cm2. The mediators released from 10(7) mastocytoma cells challenged with calcium ionophore increased Isc by 25.1 +/- 13.6 microA/cm2. In the presence of indomethacin, the Isc increased by 2.0 +/- 0.4 microA/cm2. Mastocytoma-derived mediators produced an increase in net chloride secretion without a significant effect on net sodium absorption. This study provides direct evidence that mast cell-derived mediators can stimulate epithelial ion transport in canine trachea and suggests that the effect is indirect and dependent on intact cyclooxygenase pathways in the tracheal epithelium.
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D000001:Calcimycin / Q000494:pharmacology; D004285:Dogs; D004553:Electric Conductivity; D004594:Electrophysiology; D004848:Epithelium / Q000502:physiology; D007213:Indomethacin / Q000494:pharmacology; D007477:Ions; D008168:Lung / Q000276:immunology; D008407:Mast Cells / Q000502:physiology*; D012515:Mast-Cell Sarcoma / Q000503:physiopathology; D051379:Mice; D008819:Mice, Nude; D011058:Pollen / Q000276:immunology; D011451:Prostaglandin-Endoperoxide Synthases / Q000502:physiology; D014132:Trachea / Q000502:physiology*
D007477:Ions; D000001:Calcimycin; D011451:Prostaglandin-Endoperoxide Synthases; D007213:Indomethacin