A simple method to obtain brain microtubule protein poor in microtubule-associated proteins.
By use of high glycerol and sodium chloride concentrations a microtubule protein (MTP) from porcine brain can be isolated that contains only a small portion of microtubule-associated proteins (MAPs). This "NaCl-MTP" possesses assembly properties similar to chromatographically purified tubulin.
D000818:Animals; D001923:Brain Chemistry; D046911:Macromolecular Substances; D008854:Microscopy, Electron; D008869:Microtubule-Associated Proteins / Q000302:isolation & purification*; D008870:Microtubules / Q000032:analysis* / Q000648:ultrastructure; D013552:Swine
D046911:Macromolecular Substances; D008869:Microtubule-Associated Proteins