title: Effects of oxygen on red cells during liquid storage at +4 degrees C.
abstract: Red cells collected in CPD and suspended in SAGM medium were stored in plastic (PVC) containers for 42 days at +4 degrees C. Comparison was made between aerobic storage (normal air exposure) and anaerobic storage (exposure to nitrogen gas). The air-exposed units showed a strong increase in pO2 and oxygen saturation as a result of oxygen penetration into the bags from outside. This resulted in a decrease in ATP and adenylate energy charge, a slower metabolization of adenine and hypoxanthine to AMP and IMP, respectively, and a faster decrease in red cell fluidity. To explain the findings it is concluded that aerobic storage causes an increased need of high-energy phosphate groups, possibly used for replacement of the phospholipid membrane bilayer or in repair of phosphate bonds in the cytoskeleton. It is further proposed that a slight formation of hydrogen peroxide from free oxygen radicals moderately increases the oxidation of reduced (GSH) to oxidized (GSSG) glutathione and slightly enhances the need for reduced nicotinamide-adenine dinucleotides mainly provided by increased flux through the pentose phosphate shunt.
mesh_terms: D000255:Adenosine Triphosphate / Q000097:blood; D001793:Blood Preservation / Q000379:methods*; D002245:Carbon Dioxide / Q000097:blood; D003080:Cold Temperature; D004734:Energy Metabolism; D004912:Erythrocytes / Q000378:metabolism*; D005609:Free Radicals; D005947:Glucose / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007773:Lactates / Q000096:biosynthesis; D019344:Lactic Acid; D009584:Nitrogen; D010100:Oxygen / Q000097:blood; D013997:Time Factors
chemical_list: D005609:Free Radicals; D007773:Lactates; D002245:Carbon Dioxide; D019344:Lactic Acid; D000255:Adenosine Triphosphate; D005947:Glucose; D009584:Nitrogen; D010100:Oxygen