Formation of 8-hydroxyguanine residues in cellular DNA exposed to the carcinogen 4-nitroquinoline 1-oxide.
8-Hydroxyguanine (8-OH-Gua) residues were formed in DNA of Ehrlich ascites cells exposed to the carcinogen 4-nitroquinoline 1-oxide. Formation of 8-OH-Gua was confirmed by chemical treatment of calf thymus DNA with the proximate metabolite of this carcinogen, 4-hydroxyaminoquinoline 1-oxide, together with seryl-adenosine monophosphate. The ratio of the rates of formations of 8-OH-Gua and the quinoline-bound adducts was about 0.2-0.3. A conceivable mechanism of formation of 8-OH-Gua is proposed.
D015108:4-Hydroxyaminoquinoline-1-oxide / Q000494:pharmacology; D015112:4-Nitroquinoline-1-oxide / Q000494:pharmacology*; D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000235:genetics; D002417:Cattle; D004247:DNA / Q000187:drug effects*; D006147:Guanine / Q000031:analogs & derivatives* / Q000032:analysis; D008956:Models, Chemical; D009600:Nitroquinolines / Q000494:pharmacology*
D009600:Nitroquinolines; D015108:4-Hydroxyaminoquinoline-1-oxide; D015112:4-Nitroquinoline-1-oxide; C024829:8-hydroxyguanine; D006147:Guanine; D004247:DNA