Generation of a variant t(2;8) translocation of Burkitt's lymphoma by site-specific recombination via the kappa light-chain joining signals.
We analyzed the Burkitt's lymphoma line BL64 in which a reciprocal translocation joins the immunoglobulin kappa light-chain locus on chromosome 2 to the c-myc gene on chromosome 8. The breakpoints on the two marker chromosomes 8+ and 2p- occurred 5' of the Js segment within the conserved nonamer and heptamer recombination sequences. Both signals were detected directly adjacent to the breakpoints in sequences of chromosome 8 suggesting that the translocation in BL64 was catalyzed by enzymes normally involved in V-J recombination. The distance between the c-myc gene and the breakpoint in J kappa amounts to at least 90 kilobases on the DNA level. In one allele of the c-myc gene somatic mutations were found in the promoter-leader region. This allele is transcribed and is supposed to be involved in the translocation. The half-life of the c-myc-specific mRNA in BL64 cells is not prolonged in comparison to the normal c-myc message. These results suggest that in Burkitt's lymphoma the translocation occurs during an early stage of B-cell differentiation and that in the variant translocations mechanisms other than a prolonged half-life, such as changes in transcriptional rates, or other posttranscriptional RNA processing contribute to the high steady-state level of c-myc RNA in the cytoplasm.
D001483:Base Sequence; D002051:Burkitt Lymphoma / Q000235:genetics / Q000276:immunology*; D002460:Cell Line; D002889:Chromosomes, Human, Pair 2; D002898:Chromosomes, Human, Pair 8; D005796:Genes; D014644:Genetic Variation; D006801:Humans; D007147:Immunoglobulin Light Chains / Q000235:genetics*; D007145:Immunoglobulin kappa-Chains / Q000235:genetics*; D009154:Mutation; D009693:Nucleic Acid Hybridization; D011519:Proto-Oncogenes; D012333:RNA, Messenger / Q000235:genetics; D014158:Transcription, Genetic; D014178:Translocation, Genetic
D007147:Immunoglobulin Light Chains; D007145:Immunoglobulin kappa-Chains; D012333:RNA, Messenger