Imipenem susceptibility testing with a commercially prepared dry-format microdilution tray.
MICs of imipenem, concurrently generated in commercially prepared microdilution trays containing predried antibiotic dilutions (Sensititre), and in a standard agar dilution assay (as recommended by the National Committee for Clinical Laboratory Standards, Villanova, Pa.), were within +/- 1 twofold dilution for 94% of 226 bacterial isolates. Imipenem biological activity remained stable over 5 months of tray storage at room temperature against Pseudomonas aeruginosa ATCC 27853. Activity of imipenem was shown by microdilution testing with 890 clinical isolates to be high, with only 4% of isolates having MICs of greater than or equal to 16 micrograms/ml (in vitro resistance).
D001419:Bacteria / Q000187:drug effects; D004355:Drug Stability; D004755:Enterobacteriaceae / Q000187:drug effects; D015378:Imipenem; D008826:Microbial Sensitivity Tests / Q000379:methods*; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D013696:Temperature; D013845:Thienamycins / Q000494:pharmacology*; D013997:Time Factors
D013845:Thienamycins; D015378:Imipenem