Effect of aflatoxin B1 on some liver microsomal enzymes in mice fed cyanide supplemented diets.
Aniline hydroxylase, glucose-6-phosphatase, NADPH- and NADH-cytochrome C reductase activities were measured in liver microsomes prepared from four groups of female mice. Mice were fed either control diets alone or KCN (0.357, microgram/kg body wt/day) supplemented diets or control diets plus AFB1 (0.35 microgram/kg body wt/day) administration (ip) on the 8, 9 and 10th day or the KCN supplemented diet plus AFB, administration (ip) on the 8, 9 and 10th day. KCN and AFB1 consistently elevated the activities of the enzymes. Simultaneous administration of both toxins potentiated their effects on the enzymes with the exception of glucose-6-phosphatase. Increases in microsomal protein/liver wt ratios, liver wt/body wt ratios and these enzyme activities were probably indicative of microsomal enzyme induction.
D016604:Aflatoxin B1; D000348:Aflatoxins / Q000633:toxicity*; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D003486:Cyanides / Q000633:toxicity*; D004032:Diet; D005260:Female; D008099:Liver / Q000187:drug effects; D051379:Mice; D008862:Microsomes, Liver / Q000201:enzymology*; D006899:Mixed Function Oxygenases / Q000037:antagonists & inhibitors*; D009929:Organ Size / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains
D000348:Aflatoxins; D003486:Cyanides; D016604:Aflatoxin B1; D006899:Mixed Function Oxygenases