A novel method of electrophoresis for the separation of individual human serum glycoproteins.
A method for the isolation of protein components by a combination of polyacrylamide gel electrophoresis and electro-extraction of slices of gel contained in cellophane dialysis tubing, using the H tube electrophoresis apparatus, is described. The method was applied to the purification of individual human serum glycoproteins. Although the glycoproteins have widely differing electrophoretic mobilities, they appeared to be antigenically interrelated judging from their complex "spurring" in Ouchterlony double gel diffusion tests.
D001797:Blood Protein Electrophoresis / Q000295:instrumentation / Q000379:methods; D003956:Dialysis; D004591:Electrophoresis, Polyacrylamide Gel / Q000295:instrumentation / Q000379:methods; D006023:Glycoproteins / Q000097:blood* / Q000302:isolation & purification; D006801:Humans; D005779:Immunodiffusion; D012016:Reference Values
D006023:Glycoproteins