Functional expression of plastid allophycocyanin genes in a cyanobacterium.
In Cyanophora paradoxa, the allophycocyanin apoprotein subunits, alpha and beta, are encoded in the cyanelle (plastid) genome. These genes were transferred to the cyanobacterium Synechococcus sp. PCC 7002 on a plasmid replicon. Phycobilisomes isolated from transformed cyanobacteria were found to contain C. paradoxa allophycocyanin subunits. Thus, these plastid genes are expressed in the cyanobacterium as polypeptides which become linked to a chromophore and are incorporated into the light-harvesting apparatus.
D003001:Cloning, Molecular; D000458:Cyanobacteria / Q000235:genetics; D004141:Dinoflagellida / Q000235:genetics*; D005786:Gene Expression Regulation; D005796:Genes; D007526:Isoelectric Point; D045342:Light-Harvesting Protein Complexes; D008970:Molecular Weight; D045524:Phycobilisomes; D010798:Phycocyanin / Q000235:genetics*; D010860:Pigments, Biological / Q000235:genetics*; D010940:Plant Proteins / Q000502:physiology; D010957:Plasmids
D045342:Light-Harvesting Protein Complexes; D045524:Phycobilisomes; D010860:Pigments, Biological; D010940:Plant Proteins; C002497:allophycocyanin; D010798:Phycocyanin