Subunit structure of a cortical granule lectin involved in the block to polyspermy in Xenopus laevis eggs.
The cortical granule lectin of Xenopus laevis eggs is a large molecular mass glycoprotein involved in the post-fertilization block to polyspermy. We have investigated the subunit structure of this lectin and found that the native molecule contains 10-12 monomers, each of which has considerable charge and size heterogeneity due to glycosylated side chains. In addition, significant amino acid sequence homology is indicated by peptide mapping of subunits separated by isoelectric focusing.
D000595:Amino Acid Sequence; D000818:Animals; D002241:Carbohydrates; D003432:Cross-Linking Reagents; D005260:Female; D005306:Fertilization; D005976:Glutaral; D006023:Glycoproteins / Q000302:isolation & purification; D007525:Isoelectric Focusing; D037102:Lectins; D046911:Macromolecular Substances; D008970:Molecular Weight; D010063:Ovum / Q000032:analysis*; D010446:Peptide Fragments / Q000302:isolation & purification; D029867:Xenopus Proteins; D014982:Xenopus laevis
D002241:Carbohydrates; D003432:Cross-Linking Reagents; D006023:Glycoproteins; D037102:Lectins; D046911:Macromolecular Substances; D010446:Peptide Fragments; D029867:Xenopus Proteins; C050154:cortical granule-derived lectin, Xenopus; D005976:Glutaral