[Assessment by the material and energy balance method of the directed biosynthesis of riboxin by altering the external culture conditions].
The effect of various agitation conditions on growth of the cell population of Bacillus subtilis and production of riboxin was studied. The physiological state of the culture was estimated by the relations for the respiration coefficient CO2/O2, the yield of the constructive metabolism products (X + P) by oxygen Yx + p/O2, the ratio of consumed glucose to ammonium nitrogen S/NH4+, the coefficient of the cell biosynthetic activity Yp/x and the parameter of the energetic efficiency of the substrate usage (eta). It was shown that the maximum biosynthetic activity was mainly defined by the culture state in the trophophase. The growth of the cell population in this case should proceed under conditions of conjugated aerobic metabolism. It was found that directed biosynthesis of riboxin could be provided by changing the cultivation conditions and estimation of the culture physiological state by the parameters of the material and energy balance.
D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D001412:Bacillus subtilis / Q000378:metabolism*; D001431:Bacteriological Techniques; D002245:Carbon Dioxide / Q000378:metabolism; D003470:Culture Media / Q000378:metabolism; D004734:Energy Metabolism; D005947:Glucose / Q000378:metabolism; D007290:Inosine Diphosphate / Q000096:biosynthesis*; D007292:Inosine Nucleotides / Q000096:biosynthesis*; D008433:Mathematics; D010100:Oxygen / Q000378:metabolism
D000900:Anti-Bacterial Agents; D003470:Culture Media; D007292:Inosine Nucleotides; D002245:Carbon Dioxide; D007290:Inosine Diphosphate; D005947:Glucose; D010100:Oxygen
Otsenka metodom material'no-énergeticheskogo balansa napravlennogo biosinteza riboksina pri izmenenii vneshnikh usloviĭ kul'tivirovaniia.