[Characterization by cytofluorometry of the different types of adenohypophyseal cells in the rat].
The different antehypophysical cell types which synthetize and release somatotroph (GH), corticothroph (ACTH), gonadotroph (LH-FSH) and lactotroph (PRL) hormones were analysed. The experiments were performed on hypophyses from five groups of animals: adult males, 14 days-old female, adult females, gestating adult females and lactating adult females. The cells were analysed by immunofluorescence using flow cytometry. For each of the hormones studied, there was a characteristic spectral distribution of cells. The evolution of cell size and granular content with respect to sex and physiological state of each group was studied by the analysis of diffused light. Small, slightly granular cells represented 50% of the cell population in males and 14 day-old females but only 8% in gestating or lactating females. The study of the cell cycle showed the presence of dividing cells in the population of large, granular cells from gestating and from lactating females. No features of cell division were observed in the population of small, slightly granular cells. This study indicates the potential value of multiparametric analysis in the separation of pure sub-populations of antehypophysial cells.
D000818:Animals; D002453:Cell Cycle; D002469:Cell Separation; D005260:Female; D005434:Flow Cytometry; D008297:Male; D010903:Pituitary Gland, Anterior / Q000166:cytology*; D010908:Pituitary Hormones, Anterior / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D013050:Spectrometry, Fluorescence
D010908:Pituitary Hormones, Anterior
Caractérisation par cytofluorimétrie des différents types de cellules adénohypophysaires de rats.