title: Heating cells in acid methanol for 30 min without freeze-drying provides adequate yields of fatty acids and alcohols for gas chromatographic characterization of mycobacteria.
abstract: We studied the release of mycobacterial fatty acids (as methyl esters) and secondary alcohols after heating both wet and freeze-dried cells in methanolic hydrogen chloride for different time periods. A 30-min heating of the mycobacteria without prior freeze-drying was found adequate in a routine gas chromatographic procedure for strain and species characterization.
mesh_terms: D000438:Alcohols / Q000032:analysis*; D002849:Chromatography, Gas; D005227:Fatty Acids / Q000032:analysis*; D005612:Freeze Drying; D006358:Hot Temperature; D006851:Hydrochloric Acid; D000432:Methanol; D009161:Mycobacterium / Q000032:analysis* / Q000145:classification; D009162:Mycobacterium avium / Q000032:analysis / Q000145:classification; D009169:Mycobacterium tuberculosis / Q000032:analysis / Q000145:classification
chemical_list: D000438:Alcohols; D005227:Fatty Acids; D006851:Hydrochloric Acid; D000432:Methanol