title: Occurrence and aeruginocine typing of Pseudomonas aeruginosa in buffaloes and their environment.
abstract: The occurrence and distribution of Pseudomonas aeruginosa in buffaloes and their environment was studied. Environmental sources included milkers, milking utensils, watering troughs, animal shed floor, barn-yard soil and drains. Of the different body sites examined, the organism could only be isolated from 15% of the muzzle and 5% of the belly samples. The organism was widely prevalent in the animal environment and could be isolated from milking utensils (56.67%), watering troughs (44.00%), drains (36.37%), shed floor (4.00%), barnyard soil (3.33%), and milkers' throats (50.00%) and hands before and after milking (7.14 and 10.71%, respectively). Aeruginocine typing revealed that of the 68 strains of P. aeruginosa, 65 (95.59%) were typable. Amongst the typable strains, 21 (32.30%) were classifiable and 44 (67.69%) unclassifiable. Unclassifiable pattern 23478- was most predominant and common to animals and environmental sources. Different aeruginocine types encountered were: A(2), B(4), F(1), L(1), P(4), 1(1), 8(1), 9(1), 11(3), 12(1), 14(1) and 17(1). This is of significance as most of the types encountered have been reported from clinical specimens of animal and human origin.
mesh_terms: D000818:Animals; D002020:Buffaloes / Q000382:microbiology*; D005260:Female; D006801:Humans; D007774:Lactation; D008892:Milk / Q000382:microbiology; D011247:Pregnancy; D011550:Pseudomonas aeruginosa / Q000145:classification / Q000302:isolation & purification*; D012867:Skin / Q000382:microbiology