Effects of interferons-alpha, -beta, and -gamma on human interleukin-2 production.
Interleukin-2 (IL-2) production was stimulated with staphylococcus enterotoxin A, heat-killed Staphylococcus aureus, or concanavalin A (ConA) in cultures of human peripheral blood mononuclear cells (PBMC). IL-2 was characterized by its effect on IL-2-dependent cytotoxic T lymphocytes (CTLL cells) and mouse thymocytes. Pretreatment of PBMC with greater than or equal to 100 IU/ml of human-alpha (HuIFN-alpha) or interferon-beta (HuIFN-beta), or with greater than or equal to 10 IU/ml HuIFN-gamma for 4 h enhanced the IL-2 production. The effects of 20-h IFN treatments were less pronounced or they exerted an inhibitory effect. Our results indicate that IFNs modulate IL-2 production, which in turn can mediate some of the immunomodulatory effects of IFNs.
D003208:Concanavalin A / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004768:Enterotoxins / Q000494:pharmacology; D006801:Humans; D007157:Immunologic Surveillance / Q000187:drug effects; D007370:Interferon Type I / Q000494:pharmacology*; D007371:Interferon-gamma / Q000494:pharmacology*; D007376:Interleukin-2 / Q000096:biosynthesis* / Q000302:isolation & purification; D007700:Kinetics; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects*; D008970:Molecular Weight; D013997:Time Factors
D004768:Enterotoxins; D007370:Interferon Type I; D007376:Interleukin-2; D003208:Concanavalin A; C026066:enterotoxin A, Staphylococcal; D007371:Interferon-gamma