title: Effects of a new fluorinated macrolide (P-0501A) and other erythromycins on drug metabolizing enzymes in rat liver.
abstract: The effects of a new fluorinated macrolide (P-0501A) on drug metabolizing enzymes of rat liver were compared with three erythromycins--the base, the stearate and the estolate--after 7 days of dosing (1.36 mmol/kg po daily). The three erythromycins induced the synthesis of microsomal enzymes, but the products of their metabolism inactivated cytochrome P-450 in the order base less than or equal to stearate less than estolate. N-Demethylation of erythromycin and aminopyrine increased, while O-demethylation of 4-nitroanisole was reduced and hydroxylation of aniline was not changed after in vivo treatment. Pentobarbital sleeping time was prolonged and liver glutathione levels were lower in treated rats than in controls. In contrast to the three erythromycins, P-0501A did not induce the synthesis of microsomal enzymes, did not form an inactive complex with cytochrome P-450 and did not affect mono-oxygenase activities or pentobarbital narcosis.
mesh_terms: D000284:Administration, Oral; D000633:Aminopyrine N-Demethylase / Q000096:biosynthesis; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis; D003573:Cytochrome b Group / Q000096:biosynthesis; D015786:Cytochromes b5; D004347:Drug Interactions; D004790:Enzyme Induction / Q000187:drug effects; D004917:Erythromycin / Q000031:analogs & derivatives* / Q000494:pharmacology*; D004918:Erythromycin Estolate / Q000494:pharmacology*; D005978:Glutathione / Q000378:metabolism; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D009251:NADPH-Ferrihemoprotein Reductase / Q000096:biosynthesis; D009929:Organ Size / Q000187:drug effects; D010424:Pentobarbital / Q000494:pharmacology; D051381:Rats; D012890:Sleep / Q000187:drug effects
chemical_list: D003573:Cytochrome b Group; C045925:flurithromycin; D004917:Erythromycin; D015786:Cytochromes b5; D003577:Cytochrome P-450 Enzyme System; D000633:Aminopyrine N-Demethylase; D009251:NADPH-Ferrihemoprotein Reductase; D005978:Glutathione; D010424:Pentobarbital; C011462:erythromycin stearate; D004918:Erythromycin Estolate