Phorbol ester causes short term and transient accumulation of pteridines in T cells and in cell lines.
Upon exposure to 12-O-tetradecanoylphorbol 13-acetate, interleukin 2 receptor+ T cells transiently accumulate neopterin and biopterin as was determined by HPLC after iodine oxidation of acidic cell extracts. Pteridines peak at maximally 20 fold levels after 10-20 min and return to initial levels during the following 30-40 min. Resting human peripheral blood mononuclear cells do not react within this short period. TPA elicits similar neopterin and biopterin accumulation kinetics in cell lines such as HL-60, Reh, Jurkat JMN, HeLa and 293, whereby HL-60 and Reh release substantial amounts of these transiently formed pteridines into the medium.
D001708:Biopterins / Q000031:analogs & derivatives / Q000378:metabolism; D002460:Cell Line; D006801:Humans; D007700:Kinetics; D008213:Lymphocyte Activation; D019798:Neopterin; D011493:Protein Kinase C / Q000378:metabolism; D011621:Pteridines / Q000378:metabolism*; D011971:Receptors, Immunologic / Q000378:metabolism; D015375:Receptors, Interleukin-2; D013601:T-Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D011621:Pteridines; D011971:Receptors, Immunologic; D015375:Receptors, Interleukin-2; D001708:Biopterins; D019798:Neopterin; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate