Early modifications of gene expression induced in liver by azo-dye diet.
The expression and regulation of the phosphoenolpyruvate carboxykinase gene were not grossly modified by feeding rats a 3'-methyl-4-(dimethylamino)azobenzene-containing diet despite maximum expression of the L-type pyruvate kinase gene being dramatically reduced as early as the 24th hour of the carcinogenic diet. Inhibition of aldolase B mRNA synthesis occurred more slowly, being maximum at the 3rd day. After stopping administration of the carcinogen, a very rapid, but transient increase of the L-type pyruvate kinase mRNA was observed at the 24th hour, whereas aldolase B mRNA increased only slowly. The amount of aldolase A mRNA fell quickly after termination of carcinogen administration, levels being normal at the 2nd-3rd day. At this time, the histological structure of the liver was indistinguishable from that of animals still receiving the azo-dye diet. It appears, therefore, that in the rat both administration and withdrawal of the azo-dye carcinogen induce rapid modifications of the expression of some genes, before any cellular modification is distinguishable.
D000818:Animals; D005634:Fructose-Bisphosphate Aldolase / Q000235:genetics*; D005786:Gene Expression Regulation / Q000187:drug effects*; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D008749:Methyldimethylaminoazobenzene / Q000008:administration & dosage / Q000494:pharmacology*; D010729:Phosphoenolpyruvate Carboxykinase (GTP) / Q000235:genetics*; D011770:Pyruvate Kinase / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D004124:p-Dimethylaminoazobenzene / Q000031:analogs & derivatives*
D012333:RNA, Messenger; D008749:Methyldimethylaminoazobenzene; D004124:p-Dimethylaminoazobenzene; D011770:Pyruvate Kinase; D010729:Phosphoenolpyruvate Carboxykinase (GTP); D005634:Fructose-Bisphosphate Aldolase