Immunoelectronmicroscopic localization of actin in ionophore-treated boar sperm.
Actin in ionophore-A23187 treated boar sperm has been localized by indirect immunofluorescence and immunoelectronmicroscopy (IEM), using an anti-actin monoclonal antibody. By IEM, after en bloc staining technique and treatment with 15 nm colloidal gold-IgM complex, actin was found associated with the plasma membrane (PM) and the outer acrosomal membrane vesicles and under the PM of the equatorial segment.
D000177:Acrosome / Q000648:ultrastructure; D000199:Actins / Q000378:metabolism*; D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D002462:Cell Membrane / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D006046:Gold; D007425:Intracellular Membranes / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D013094:Spermatozoa / Q000187:drug effects / Q000648:ultrastructure*; D013552:Swine
D000199:Actins; D000001:Calcimycin; D006046:Gold