Effects of selenalysine on thialysine resistant CHO cells.
A thialisyne resistant variant clone of CHO cells also shows a lower sensitivity to selenasyne, the lysine analog containing selenium. Growth rate, cell viability and protein synthesis rate are less affected by selenasyne in the variant compared to the parental strain. Data are reported showing that during cellular growth of either strain some toxic derivatives of selenasyne are produced and accumulated in the culture medium even in the presence of excess lysine.
D000818:Animals; D002455:Cell Division / Q000187:drug effects*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002999:Clone Cells; D003470:Culture Media; D003545:Cysteine / Q000031:analogs & derivatives* / Q000494:pharmacology; D004351:Drug Resistance; D008239:Lysine / Q000031:analogs & derivatives* / Q000494:pharmacology; D016566:Organoselenium Compounds; D014176:Protein Biosynthesis; D012643:Selenium / Q000494:pharmacology*
D003470:Culture Media; D016566:Organoselenium Compounds; C000204:S-2-aminoethyl cysteine; C011351:selenalysine; D012643:Selenium; D008239:Lysine; D003545:Cysteine