Prostaglandins in the semicircular canal of the frog.
The synthesis of prostaglandins by ampulla and duct tissue isolated from the frog posterior semicircular canal was investigated in vitro. Ampulla and duct produced PGE2 (9 and 6 pg/structure, respectively) and prostacyclin (26 and 12 pg/structure). In the ampulla, prostaglandins mostly originated from the part containing dark and sensory cells and was not altered by 10(-3) M streptomycin. Prostaglandin levels were time-dependent and temperature-dependent. Arachidonic acid (3 X 10(-5) M) stimulated PGI2 synthesis by ampulla and duct (by 11.4 and 17 times) and PGE2 synthesis by 50 times in both structures. Ionophore A23187 stimulated ampulla and duct PGI2 synthesis (by 4.8 and 5.6 times) and PGE2 synthesis (by 2.4 and 1.8 times). Subcutaneous 100 mg/kg aspirin reduced PGI2 and PGE2 synthesis (ampulla: -87%, -33%; duct: -100%, -33%). Indomethacin (10(-6) M), in vitro, decreased PGI2 and PGE2 synthesis (ampulla: -47%, -47%; duct; -22%, -77%). Within 3 h, aspirin (5 X 10(-6) M) or arachidonic acid (2 X 10(-5) M) did not change Na and K concentrations in endolymph. It is concluded that frog inner ear produces PGI2 and PGE2, mostly from the part containing the dark cells, and that prostaglandins could be involved in the physiology of inner ear.
D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000494:pharmacology; D001241:Aspirin / Q000494:pharmacology; D000001:Calcimycin / Q000494:pharmacology; D015232:Dinoprostone; D011464:Epoprostenol / Q000096:biosynthesis*; D066298:In Vitro Techniques; D007213:Indomethacin / Q000494:pharmacology; D011188:Potassium / Q000378:metabolism; D011458:Prostaglandins E / Q000096:biosynthesis*; D011893:Rana esculenta; D012665:Semicircular Canals / Q000187:drug effects / Q000378:metabolism*; D012964:Sodium / Q000378:metabolism; D013307:Streptomycin / Q000494:pharmacology
D001095:Arachidonic Acids; D011458:Prostaglandins E; D016718:Arachidonic Acid; D000001:Calcimycin; D012964:Sodium; D011464:Epoprostenol; D015232:Dinoprostone; D001241:Aspirin; D011188:Potassium; D007213:Indomethacin; D013307:Streptomycin