Fluorescence properties of allophycocyanin and a crosslinked allophycocyanin trimer.
Data on the wavelength and temperature dependence of both time-resolved and steady state fluorescence emission are presented for allophycocyanin (AP) and for a crosslinked allophycocyanin trimer (XL-AP) (Ong LJ and Glazer AN: Physiol Veg 23:777-787, 1985). AP dissociates at high dilution and is not stable above 40 degrees C even at moderate protein concentration. In contrast, XL-AP does not dissociate even at very low protein concentrations and is completely stable up to 60 degrees C in the presence of 0.75 M NaK-phosphate, pH 7.0. The results show that XL-AP is superior to AP for use in conjugates that absorb and emit in the red region of the spectrum. The high stability of XL-AP at elevated temperatures at high phosphate concentrations suggests that this derivative may be useful in conjunction with nucleic acid probes.
D003201:Computers; D000458:Cyanobacteria; D005453:Fluorescence; D005456:Fluorescent Dyes; D007700:Kinetics; D010798:Phycocyanin; D010860:Pigments, Biological; D011108:Polymers; D013050:Spectrometry, Fluorescence; D013696:Temperature
D005456:Fluorescent Dyes; D010860:Pigments, Biological; D011108:Polymers; C002497:allophycocyanin; D010798:Phycocyanin