[Analysis of the frequency of allelic exclusion of immunoglobulin light chain genes and molecular characteristics of immunoglobulins secreted by hybridomas with expression of both allelic genes].
The investigation of 750 B-lymphocyte hybridoma clones obtained by fusion of mouse myeloma and newborn heterozygous Igk-la/Igk-1b rat splenocytes has revealed that 9,8% of Ig kappa-chain genes are rearranged productively. Seventeen hybridomas secrete kappa-chains of both allelic variants. The analysis of IgM molecules of nine such clones demonstrated that in six cases only one L-chain allotype is present in IgM. Thus for the first time the high frequency of selective association of H and L chains was shown. Evidently this selectively may function as one of the allelic exclusion mechanisms at the Ig assembly stage.
D000483:Alleles; D000818:Animals; D005455:Fluorescent Antibody Technique; D006825:Hybridomas / Q000276:immunology; D007126:Immunoglobulin Allotypes / Q000235:genetics*; D007143:Immunoglobulin Heavy Chains / Q000032:analysis / Q000235:genetics; D007147:Immunoglobulin Light Chains / Q000032:analysis / Q000235:genetics*; D010455:Peptides / Q000032:analysis; D011863:Radioimmunoassay; D051381:Rats
D007126:Immunoglobulin Allotypes; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D010455:Peptides
Analiz chastoty allel'nogo iskliucheniia genov legkikh tsepeĭ immunoglobulinov i molekuliarnaia kharakteristika immunoglobulinov, sekretiruemykh gibridomami s ékspressieĭ oboikh allel'nykh genov.