Isolation and oncogenic potential of a novel human src-like gene.
We have isolated cDNA molecules representing the complete coding sequence of a new human gene which is a member of the src family of oncogenes. Nucleotide sequence analysis revealed that this gene, termed slk, encoded a 537-residue protein which was 86% identical to the chicken proto-oncogene product, p60c-src, over a stretch of 191 amino acids at its carboxy terminus. In contrast, only 6% amino acid homology was observed within the amino-terminal 82 amino acid residues of these two proteins. It was possible to activate slk as a transforming gene by substituting approximately two-thirds of the slk coding sequence for an analogous region of the v-fgr onc gene present in Gardner-Rasheed feline sarcoma virus. The resulting hybrid protein molecule expressed in transformed cells demonstrated protein kinase activity with specificity for tyrosine residues.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002478:Cells, Cultured; D004247:DNA / Q000032:analysis; D004727:Endothelium; D005796:Genes; D006801:Humans; D051379:Mice; D015688:Oncogene Protein pp60(v-src); D009857:Oncogenes; D000090063:Proto-Oncogene Mas; D012191:Retroviridae Proteins / Q000235:genetics*
C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D012191:Retroviridae Proteins; D004247:DNA; D015688:Oncogene Protein pp60(v-src)