title: Immunochemical evidence for interaction between cholecystokinin C-terminal peptides and the protein inhibitor of adenosine 3',5'-cyclic monophosphate dependent protein kinase in hog terminal bile ducts.
abstract: The interaction of cholecystokinin (CCK) C-terminal peptides with the protein inhibitor (PK-I) of adenosine 3',5'-cyclic monophosphate-dependent protein kinase (A-PK) was studied by immunoprecipitation using anti-PK-I antibody. The binding of CCK-4 peptide to PK-I was shown to be specific; while CCK-4 enhanced precipitation of PK-I with antibody, it did not enhance precipitation of bovine serum albumin (BSA) by anti-BSA. The interaction of CCK-4 with PK-I is reversible and similar to that of urea but different from that of alkali. These results support the conclusion that CCK-C terminal peptides interact specifically with PK-I.
mesh_terms: D000818:Animals; D001652:Bile Ducts / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism*; D011232:Chemical Precipitation; D002766:Cholecystokinin / Q000378:metabolism* / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D006168:Guinea Pigs; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D047908:Intracellular Signaling Peptides and Proteins; D010446:Peptide Fragments; D011485:Protein Binding; D012710:Serum Albumin, Bovine / Q000276:immunology / Q000378:metabolism; D013552:Swine; D014508:Urea / Q000494:pharmacology
chemical_list: D002352:Carrier Proteins; D047908:Intracellular Signaling Peptides and Proteins; D010446:Peptide Fragments; C015482:protein kinase modulator; D012710:Serum Albumin, Bovine; D014508:Urea; D002766:Cholecystokinin