Demonstration of monoamine oxidase type B in serotonergic and type A in noradrenergic neurons in the cat dorsal pontine tegmentum by an improved histochemical technique.
Application of a diaminobenzidine-coupled peroxidation method to monoamine oxidase (MAO) histochemistry demonstrates a detailed morphology of MAO-containing perikarya, dendrites, axons and fibers in the dorsal pontine tegmentum of the cat, sectioned with a vibratome. Further, utilization of specific inhibitors, deprenyl and clorgyline, also provides evidence that serotonergic neurons contain exclusively MAO-B and catecholaminergic ones contain MAO-A.
D000818:Animals; D001369:Axons / Q000201:enzymology; D002415:Cats; D003010:Clorgyline / Q000494:pharmacology*; D003712:Dendrites / Q000201:enzymology; D006651:Histocytochemistry; D006735:Horseradish Peroxidase; D008995:Monoamine Oxidase / Q000032:analysis*; D009638:Norepinephrine; D010627:Phenethylamines / Q000494:pharmacology*; D011149:Pons / Q000201:enzymology*; D011437:Propylamines / Q000494:pharmacology*; D012642:Selegiline / Q000494:pharmacology*; D012701:Serotonin; D004124:p-Dimethylaminoazobenzene
D010627:Phenethylamines; D011437:Propylamines; D012642:Selegiline; D012701:Serotonin; D004124:p-Dimethylaminoazobenzene; D006735:Horseradish Peroxidase; D008995:Monoamine Oxidase; D003010:Clorgyline; D009638:Norepinephrine