Morphology of tight junctions in the ciliary epithelium of rabbits during arachidonic acid-induced breakdown of the blood-aqueous barrier.
A reversible breakdown of the blood-aqueous barrier in the iridial processes of rabbits has been induced by arachidonic acid as demonstrated by the passage of horseradish peroxidase at places through the tight junctions. Freeze-fracture images reveal very discontinuous P-face ridges. However, the analysis of complementary replicas demonstrates that discontinuities of P-face ridges are always complemented by particles or short bars found in the E-face furrows. Though the problem exists of correlating freeze-fracture images of the junctional structure to the focal passage of horseradish peroxidase, the data suggest that the discontinuities of P-face ridges cannot be the structural counterpart of the passage of horseradish peroxidase. Alternative pathways of horseradish peroxidase are discussed in context with the offset bifibrillary model of the junction.
D000818:Animals; D001082:Aqueous Humor / Q000502:physiology*; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000494:pharmacology*; D002924:Ciliary Body / Q000098:blood supply / Q000187:drug effects / Q000648:ultrastructure*; D004848:Epithelium / Q000187:drug effects / Q000648:ultrastructure; D005614:Freeze Fracturing; D007365:Intercellular Junctions / Q000187:drug effects / Q000648:ultrastructure*; D007498:Iris / Q000098:blood supply / Q000187:drug effects / Q000648:ultrastructure*; D008854:Microscopy, Electron; D011817:Rabbits
D001095:Arachidonic Acids; D016718:Arachidonic Acid