title: The changing ratio of serum bioactive to immunoreactive follicle-stimulating hormone in normal men following treatment with a potent gonadotropin releasing hormone antagonist.
abstract: Using a recently developed granulosa cell aromatase bioassay (GAB), we measured serum bioactive follicle-stimulating hormone (bio-FSH) levels in 5 normal men after administration of a potent GnRH antagonist. Although only minimal suppression of immunoreactive FSH (immuno-FSH) was detected during administration of 20 mg of the antagonist, [N-Ac-D-Nal(2)1,D-pCl-Phe2,D-Trp3,D-h(Arg(Et2)6,D-Ala10]GnRH , pronounced inhibition (79 to 89%) of bio-FSH levels occurred. Concomitantly, the ratio of bio- to immuno-FSH levels dramatically decreased within 6 h after antagonist administration. These data reinforce earlier expectations that GnRH antagonists might be potential male contraceptives and provide the first report of changes in circulating bio- to immuno-FSH levels. The GAB assay will facilitate future studies on the biochemical mechanisms by which GnRH antagonists induce changes in the bioactivity of circulating FSH.
mesh_terms: D000328:Adult; D001141:Aromatase / Q000378:metabolism; D001681:Biological Assay; D005260:Female; D005640:Follicle Stimulating Hormone / Q000097:blood*; D007987:Gonadotropin-Releasing Hormone / Q000031:analogs & derivatives* / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D006107:Granulosa Cells / Q000201:enzymology; D006801:Humans; D008297:Male; D008875:Middle Aged; D011863:Radioimmunoassay
chemical_list: C043699:detirelix; D007987:Gonadotropin-Releasing Hormone; D005640:Follicle Stimulating Hormone; D001141:Aromatase