Effect of mitomycin C on the activation of adenylate cyclase in rat ascites hepatoma AH130 cells.
Isoproterenol (IPN)-stimulated activity of adenylate cyclase was enhanced in a dose-dependent manner by exposure of AH130 cells to mitomycin C (MMC). The enhancement was also observed in prostaglandin E1-, guanine nucleotide analog-, NaF-, cholera toxin- and forskolin-stimulated activities of the enzyme but not in manganese-stimulated activity. In addition, even when the cells pretreated with islet-activating protein were exposed to MMC, IPN-stimulated activity of adenylate cyclase was enhanced. Anaerobic exposure of AH130 cells to MMC somewhat inhibited IPN-stimulated activity of adenylate cyclase in contrast with aerobic exposure. Exposure of cells to adriamycin also caused enhancement of IPN-stimulated activity of adenylate cyclase but exposure to nitrogen mustard inhibited the enzyme stimulation by IPN. The enhancing effect of MMC was lost by the combined treatment with alpha-tocopherol. From these results, it was shown that MMC modulated the activity of adenylate cyclase, probably through alterations in membrane structure.
D000262:Adenylyl Cyclases / Q000378:metabolism*; D000332:Aerobiosis; D000693:Anaerobiosis; D000818:Animals; D004347:Drug Interactions; D004789:Enzyme Activation / Q000187:drug effects; D005260:Female; D066298:In Vitro Techniques; D007545:Isoproterenol / Q000494:pharmacology; D008114:Liver Neoplasms, Experimental / Q000201:enzymology*; D008466:Mechlorethamine / Q000494:pharmacology; D016685:Mitomycin; D008937:Mitomycins / Q000494:pharmacology*; D051381:Rats
D008937:Mitomycins; D008466:Mechlorethamine; D016685:Mitomycin; D000262:Adenylyl Cyclases; D007545:Isoproterenol