L-[3H]lysine binding to rat retinal membrane: II. Effect of kainic acid, D,L-alpha-aminoadipic acid, iodoacetic acid, and modification by dark-exposure.
The rat retina and the different brain regions contain membranes sites that bind L-lysine in the nanomolar range. These binding sites undergo changes in different experimental conditions, thus: intraocular injection of kainic acid induces a reduction of the density of L-lysine binding sites, D,L-alpha-aminoadipic acid injected into the eye enhances both kinetic parameters (Bmax and Kd) of L-[3H]lysine binding sites, the intraperitoneal injection of iodoacetic acid decreases the sensitivity for its ligand binding sites, and the exposure to darkness of the rats reduces L-[3H]lysine binding in the retina, thalamus, hypothalamus and superior colliculus, but not in the occipital cortex; such a decrease appears to be characterized, at least in the retina, by a lower sensitivity of the binding sites for L-lysine after the exposure to darkness. The results show that L-lysine binding sites are located on kainic acid-sensitive cells and can be involved in the physiological mechanism of vision.
D015074:2-Aminoadipic Acid / Q000494:pharmacology*; D000600:Amino Acids, Dicarboxylic / Q000494:pharmacology*; D000818:Animals; D001921:Brain / Q000378:metabolism; D003624:Darkness; D007461:Iodoacetates / Q000494:pharmacology*; D019807:Iodoacetic Acid; D007608:Kainic Acid / Q000494:pharmacology*; D007700:Kinetics; D008239:Lysine / Q000378:metabolism*; D008297:Male; D051381:Rats; D012160:Retina / Q000378:metabolism*; D014795:Visual Pathways / Q000378:metabolism
D000600:Amino Acids, Dicarboxylic; D007461:Iodoacetates; D015074:2-Aminoadipic Acid; D008239:Lysine; D007608:Kainic Acid; D019807:Iodoacetic Acid