title: A rapid method for detection of tyrosinase activity in electrophoresis.
abstract: This rapid and sensitive method for localizing tyrosinase in polyacrylamide slab gels is based on the condensation of Bestthorn's hydrazone (3 methyl-2-benzothiazolinone hydrazone hydrochloride) with the quinone obtained by enzymatic oxidation of phenol. Both monophenolase and diphenolase activities are localized by this method.
mesh_terms: D000818:Animals; D052160:Benzothiazoles; D004156:Catechol Oxidase / Q000032:analysis*; D002396:Catechols; D003445:Crustacea; D004591:Electrophoresis, Polyacrylamide Gel; D006835:Hydrazones; D014442:Monophenol Monooxygenase / Q000032:analysis*; D013844:Thiazoles
chemical_list: D052160:Benzothiazoles; D002396:Catechols; D006835:Hydrazones; D013844:Thiazoles; D004156:Catechol Oxidase; D014442:Monophenol Monooxygenase; C002536:3-methyl-2-benzothiazolone hydrazone