[Adhesion of Corynebacterium diphtheriae].
The conditions for the direct hemagglutination test performed to determine the degree of adhesion of C. diphtheriae were defined. For this test sheep red blood cells, trypsin-treated ex tempore, were used. Only newly isolated cultures, subcultured for not more than 2-5 times and stored for not more than 2-7 days or freeze-dried, were employed. The culture to be tested was grown in nutrient agar with 10% of normal horse serum. The test was made in microtitrator round-bottom wells. The mixture of different dilutions of the culture was incubated for 2 hours at 37 degrees C, then left overnight at 4 degrees C. All 147 newly isolated or freeze-dried C. diphtheriae strains under test had different degrees of adhesion. Their adhesive activity was unrelated to their biovar. Toxigenic strains were significantly more active in hemagglutination (53.5 +/- 3.0%) than nontoxigenic ones (23.5 +/- 3.9%). The strains isolated from the nose, irrespective of their biological properties, were more active than those isolated from the pharynx.
D000818:Animals; D001422:Bacterial Adhesion; D001431:Bacteriological Techniques; D003353:Corynebacterium diphtheriae / Q000302:isolation & purification / Q000472:pathogenicity*; D004167:Diphtheria Toxin / Q000096:biosynthesis; D004912:Erythrocytes / Q000382:microbiology; D006386:Hemagglutination Tests; D006801:Humans; D009666:Nose / Q000382:microbiology; D010614:Pharynx / Q000382:microbiology; D011309:Preservation, Biological; D013997:Time Factors
D004167:Diphtheria Toxin
Adgeziia Corynebacterium diphtheriae.