[Isolation by binding to ultrafiltration membranes of a testicular factor limiting the number of germ cells in fetal ovary of rats in vitro].
The medium used for culturing in vitro fetal or neonatal testes, when used subsequently to culture for 4 days ovaries from 13.5 day old rat fetuses, has the property of severely limiting the number of ovarian germ cells. The non-dialysable factor(s) responsible for the observed effect binds to ultrafiltration membranes (Diaflo, Amicon) and can be eluted from these membranes with fresh medium added 1 M NaCl.
D000818:Animals; D002452:Cell Count; D002478:Cells, Cultured; D003470:Culture Media; D005260:Female; D008297:Male; D008851:Micropore Filters; D010053:Ovary / Q000166:cytology / Q000196:embryology*; D010063:Ovum / Q000166:cytology*; D051381:Rats; D013737:Testis / Q000196:embryology / Q000502:physiology*; D014462:Ultrafiltration
D003470:Culture Media
Isolement par liaison à des membranes d'ultrafiltration, d'un facteur testiculaire limitant le nombre de cellules germinales dans l'ovaire foetal de rat in vitro.