Cytological evidence for single-cell origin of tumors induced with 3-methylcholanthrene in female mice carrying Cattanach's translocation.
Chromosome studies were carried out on 12 tumors induced by subcutaneous injection of 0.005-0.5 mg of 3-methylcholanthrene in female mice carrying Cattanach's X-autosome translocation. Using the asynchronously replicating (hence genetically inactive) X chromosome as a marker, we obtained evidence showing that most or all of these tumors were monoclonal in origin. A single case, in which cell fusion occurred early in tumorigenesis, demonstrated that simultaneous expression of two different X chromosomes is not always incompatible with monoclonality.
D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000139:chemically induced / Q000235:genetics / Q000473:pathology; D004303:Dosage Compensation, Genetic; D005260:Female; D005354:Fibrosarcoma / Q000139:chemically induced / Q000235:genetics / Q000473:pathology; D005819:Genetic Markers; D007621:Karyotyping; D008748:Methylcholanthrene; D051379:Mice; D009374:Neoplasms, Experimental / Q000139:chemically induced / Q000235:genetics* / Q000473:pathology; D014411:Neoplastic Stem Cells / Q000473:pathology* / Q000648:ultrastructure; D012208:Rhabdomyosarcoma / Q000139:chemically induced / Q000235:genetics / Q000473:pathology; D014178:Translocation, Genetic
D005819:Genetic Markers; D008748:Methylcholanthrene