title: Handling, labeling, and fractionating sea urchin spermatozoa.
mesh_terms: D000177:Acrosome / Q000378:metabolism / Q000502:physiology; D000818:Animals; D000954:Antigens, Surface / Q000032:analysis / Q000276:immunology; D002452:Cell Count / Q000379:methods; D002458:Cell Fractionation / Q000379:methods; D002462:Cell Membrane / Q000032:analysis; D002469:Cell Separation / Q000379:methods*; D003470:Culture Media; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D005456:Fluorescent Dyes; D007457:Iodine Radioisotopes; D037102:Lectins; D008297:Male; D008846:Micromanipulation / Q000379:methods*; D010101:Oxygen Consumption; D010761:Phosphorus Radioisotopes; D011309:Preservation, Biological; D012617:Sea Urchins / Q000166:cytology*; D012621:Seasons; D012623:Seawater; D012661:Semen / Q000166:cytology; D013082:Sperm Tail; D013094:Spermatozoa / Q000032:analysis / Q000378:metabolism / Q000502:physiology*; D013535:Suspensions; D013861:Thiocyanates
chemical_list: D000954:Antigens, Surface; D003470:Culture Media; D005452:Fluoresceins; D005456:Fluorescent Dyes; D007457:Iodine Radioisotopes; D037102:Lectins; D010761:Phosphorus Radioisotopes; D013535:Suspensions; D013861:Thiocyanates; D016650:Fluorescein-5-isothiocyanate