High pressure liquid chromatographic determination of carbocisteine in human plasma and urine.
A method is described for the direct analysis of the amino acid carbocisteine in plasma and urine samples, following reaction with dabsyl chloride. Dabsylated carbocisteine is subjected to high pressure liquid chromatography with spectrophotometric detection at 425 nm. The usefulness of the method for bioavailability studies is discussed and compared with methods currently in use.
D001682:Biological Availability; D002233:Carbocysteine / Q000032:analysis* / Q000097:blood / Q000652:urine; D002851:Chromatography, High Pressure Liquid; D003545:Cysteine / Q000031:analogs & derivatives*; D004355:Drug Stability; D006801:Humans; D006863:Hydrogen-Ion Concentration; D004124:p-Dimethylaminoazobenzene / Q000031:analogs & derivatives
C010572:4-N,N-dimethylaminoazobenzene-4'-sulfonyl chloride; D002233:Carbocysteine; D004124:p-Dimethylaminoazobenzene; D003545:Cysteine