Isolation of Coccidioides immitis F antigen by immunoaffinity chromatography with monospecific antiserum.
Detection of antibody to Coccidioides immitis F antigen is of proved value in the diagnosis of coccidioidomycosis. This antibody is demonstrable by use of an immunodiffusion assay with reference coccidioidin antigen and antiserum to C. immitis. Using a combination of lectin affinity and immunoaffinity chromatography, we isolated the F antigen from coccidioidin and prepared monospecific antibody to the purified antigen. The availability of these reagents will enable the development of a sensitive and specific assay for detecting serologic reactivity to this antigen.
D000907:Antibodies, Bacterial; D000918:Antibody Specificity; D000942:Antigens, Bacterial / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D003045:Coccidioides / Q000276:immunology*; D003208:Concanavalin A / Q000378:metabolism; D005779:Immunodiffusion; D007123:Immunoelectrophoresis, Two-Dimensional; D007163:Immunosorbent Techniques
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D003208:Concanavalin A