title: New method for typing Staphylococcus aureus resistant to methicillin based on sulphur-35 methionine labelled proteins: its application in an outbreak.
abstract: Sodium dodecyl sulphate polyacrylamide gel electrophoresis of sulphur-35 methionine labelled cellular proteins of Staphylococcus aureus resistant to methicillin was used as a typing method during an outbreak on a cardiothoracic ward. This showed that the outbreak strain was indistinguishable from the epidemic strain of methicillin resistant S aureus prevalent in London. In contrast, 44 epidemiologically separate strains of S aureus gave individually distinct radiolabelled protein profiles. This method permitted rapid confirmation that an epidemic strain was responsible and indicated the need for urgent control measures.
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism; D001431:Bacteriological Techniques; D003428:Cross Infection / Q000382:microbiology; D004196:Disease Outbreaks / Q000453:epidemiology*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008131:London; D008712:Methicillin; D008715:Methionine; D010403:Penicillin Resistance; D013203:Staphylococcal Infections / Q000453:epidemiology* / Q000382:microbiology; D013211:Staphylococcus aureus / Q000145:classification* / Q000378:metabolism; D013462:Sulfur Radioisotopes
chemical_list: D001426:Bacterial Proteins; D013462:Sulfur Radioisotopes; D008715:Methionine; D008712:Methicillin