Direct evidence for the participation of pyruvate in N-hydroxylation of lysine.
The contribution of pyruvate to the formation of N6-acetyl-N6-hydroxylysine by a cell-free system of Aerobacter aerogenes 62-1 involved in the production of the dihydroxamate siderophore, aerobactin, has been assessed by a study of the influence of its analogs as well as of inhibitors of thiamine pyrophosphate-dependent decarboxylation reactions. These studies have provided unequivocal evidence for pyruvate functioning not only as a source of reducing equivalents in the initial step of N-hydroxylation of lysine but also as a precursor of the acetyl moiety in the subsequent conversion of the N-hydroxy amino to its N6-acetyl derivative.
D002384:Catalysis; D004754:Enterobacter / Q000378:metabolism; D006900:Hydroxylation; D008239:Lysine / Q000031:analogs & derivatives / Q000096:biosynthesis / Q000378:metabolism*; D011773:Pyruvates / Q000378:metabolism*; D019289:Pyruvic Acid
D011773:Pyruvates; D019289:Pyruvic Acid; C049468:N(6)-acetyl-N(6)-hydroxylysine; D008239:Lysine