Biochemical characterization of the epithelial basement membrane antigen defined by the monoclonal antibody KF-1.
The KF-1 monoclonal antibody has been shown to identify a noncollageneous component of the lamina densa of the basement membrane zone of human skin. In the present work, the monoclonal antibody and the antigen were further characterized. KF-1 monoclonal antibody is an IgG3 immunoglobulin with no binding to staphylococcal protein A. Two squamous carcinoma cell lines--namely, TR131 and TR146--quantitatively express the antigen. Immunofluorescence techniques showed that the antigen is a cell surface antigen, is sensitive to Triton X-100 extraction, and is concentrated in cell areas from which actin fibers are excluded. Immunoblot analysis showed that this monoclonal antibody identifies a 72 kD polypeptide present in TR131 cell extract as well as in cultured human keratinocyte cell extract.
D000911:Antibodies, Monoclonal; D000941:Antigens / Q000032:analysis*; D001485:Basement Membrane / Q000276:immunology; D002294:Carcinoma, Squamous Cell / Q000276:immunology; D002460:Cell Line; D003101:Collodion; D004591:Electrophoresis, Polyacrylamide Gel; D004817:Epidermis / Q000276:immunology*; D004848:Epithelium / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006801:Humans; D005779:Immunodiffusion; D007074:Immunoglobulin G; D013205:Staphylococcal Protein A
D000911:Antibodies, Monoclonal; D000941:Antigens; D007074:Immunoglobulin G; D013205:Staphylococcal Protein A; D003101:Collodion