Interferon-alpha, beta and -gamma induce (2'-5') oligoadenylate synthetase in cultured mouse brain cells.
Basal and interferon (IFN)-induced levels of (2'-5') oligoadenylate synthetase activity were measured in astrocyte cultures from the mouse cerebral cortex, in neurone-enriched and mixed cerebellar cultures, and in two continuous neural cell lines by a radioimmunoassay procedure. All untreated cultures contained measureable enzyme activity. Both purified IFN-alpha, beta and recombinant IFN-gamma induced the enzyme in all cultures with the exception of the C8S cell line which did not respond to IFN-gamma. IFN-alpha, beta was more effective than IFN-gamma. The amplitude of induction by IFN-alpha, beta was highest in the cell lines, intermediate in cortical astrocytes and lowest in mixed and neurone-enriched cultures from the cerebellum.
D015088:2',5'-Oligoadenylate Synthetase / Q000378:metabolism*; D000818:Animals; D001253:Astrocytes / Q000201:enzymology; D001921:Brain / Q000201:enzymology*; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology / Q000201:enzymology; D002540:Cerebral Cortex / Q000166:cytology / Q000201:enzymology; D007370:Interferon Type I / Q000494:pharmacology*; D007371:Interferon-gamma / Q000494:pharmacology*; D051379:Mice; D008815:Mice, Inbred Strains; D009474:Neurons / Q000201:enzymology; D011863:Radioimmunoassay
D007370:Interferon Type I; D007371:Interferon-gamma; D015088:2',5'-Oligoadenylate Synthetase