Detection of mycobacterial antigens in leprosy serum immune complex.
The antigens from immune complexes of sera from patients with mycobacterial diseases were released by sodium dodecyl sulfate. The antigenic activity of the released proteins was tested by agar gel diffusion and immunoelectrophoresis. This simple method provided direct evidence for the presence of mycobacterial antigens in the immune complexes of sera from patients with leprosy and tuberculosis.
D000936:Antigen-Antibody Complex / Q000276:immunology*; D000942:Antigens, Bacterial / Q000032:analysis*; D006801:Humans; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D007918:Leprosy / Q000276:immunology*; D009166:Mycobacterium leprae / Q000276:immunology*; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D012967:Sodium Dodecyl Sulfate; D014397:Tuberculosis, Pulmonary / Q000276:immunology
D000936:Antigen-Antibody Complex; D000942:Antigens, Bacterial; D012967:Sodium Dodecyl Sulfate