Partial characterization of a mitomycin-C resistant T cell.
A cell resistant to concentrations of mitomycin-C associated with the inactivation of many in vitro immune responses is described. This cell is responsive to concanavalin A (Con A), poorly adherent to nylon wool, present in relatively greater numbers in lymph node than other lymphoid tissues, and is relatively radiosensitive. In part, the resistance of this T cell appears to relate to a reduced activity of a microsomal reductase responsible for converting mitomycin-C to its more active form. Discrepancies in reductase activity may represent a way to differentiate among subsets of lymphocytes.
D000818:Animals; D001482:Base Composition; D003208:Concanavalin A / Q000494:pharmacology; D004247:DNA / Q000032:analysis; D004305:Dose-Response Relationship, Drug; D004351:Drug Resistance; D008198:Lymph Nodes / Q000378:metabolism; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D016685:Mitomycin; D008937:Mitomycins / Q000378:metabolism / Q000494:pharmacology*; D010088:Oxidoreductases / Q000032:analysis; D013154:Spleen / Q000378:metabolism; D013601:T-Lymphocytes / Q000187:drug effects*
D008937:Mitomycins; D003208:Concanavalin A; D016685:Mitomycin; D004247:DNA; D010088:Oxidoreductases