title: The preparation and in vivo behaviour of 111In-oxine labelled neutrophils separated from whole blood using mono-poly resolving medium.
abstract: using a single step separation procedure, we have developed a method for labelling human neutrophils with 111In-oxine. This method allows a rapid separation of neutrophils from whole blood, with negligible mononuclear or red cell contamination. Preliminary studies using 111In-labelled neutrophils show minimal lung retention and early accumulation in the spleen consistent with viable cells. In addition, focal accumulation of 111In has been imaged in patients with localized inflammation or sepsis.
mesh_terms: D002469:Cell Separation / Q000379:methods*; D006801:Humans; D006912:Hydroxyquinolines; D007204:Indium; D007249:Inflammation / Q000097:blood; D007553:Isotope Labeling / Q000379:methods; D009504:Neutrophils / Q000502:physiology*; D015125:Oxyquinoline; D011868:Radioisotopes
chemical_list: D006912:Hydroxyquinolines; D011868:Radioisotopes; D007204:Indium; D015125:Oxyquinoline