Microsomal arachidonate bioconversion in rat small intestine during maturation.
This study was designed to compare prostaglandin (PG)-synthesizing activity in rat small intestinal microsomes in the 1st, 3rd, and 6th weeks of life and at maturity (greater than 100 days). When an incubation system was used containing 2 mg microsomal protein, 0.5 mM (-)-epinephrine and 1 mM reduced glutathione, the highest PG-synthesizing activity was achieved by incubating 0.157 mM 1-(14)C-arachidonate (specific activity 2.6 X 10(6) dpm/mumol) at 37 degrees C for 5 min. The labeled metabolites were extracted and then separated with high performance liquid chromatography. The four PGs analyzed were 6-keto-prostaglandin F1 alpha (6-keto-PGF1 alpha), thromboxane B2, prostaglandin F2 alpha and prostaglandin E2. Enzymatic activity for the synthesis of 6-keto-PGF1 alpha was much higher than that for the other PGs. A significant difference was observed for the bioconversion from arachidonate to 6-keto-PGF1 alpha and total PGs among the four age groups of rats. The postweanling groups (week 6 and adult) showed significantly higher enzymatic activities for the syntheses of 6-keto-PGF1 alpha and total PGs than did the preweanling groups (weeks 1 and 3).
D015121:6-Ketoprostaglandin F1 alpha / Q000096:biosynthesis; D000367:Age Factors; D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism*; D015237:Dinoprost; D015232:Dinoprostone; D066298:In Vitro Techniques; D007421:Intestine, Small / Q000254:growth & development / Q000378:metabolism*; D008861:Microsomes / Q000378:metabolism; D011458:Prostaglandins E / Q000096:biosynthesis; D011460:Prostaglandins F / Q000096:biosynthesis; D051381:Rats; D011919:Rats, Inbred Strains; D013929:Thromboxane B2 / Q000096:biosynthesis
D001095:Arachidonic Acids; D011458:Prostaglandins E; D011460:Prostaglandins F; D016718:Arachidonic Acid; D013929:Thromboxane B2; D015121:6-Ketoprostaglandin F1 alpha; D015237:Dinoprost; D015232:Dinoprostone