[Reconstitution of high-affinity galactose transport of Salmonella typhimurium in proteoliposomes: energization by lipoamide and NAD or by the membrane potential; inhibition by ATP].
The binding protein-dependent galactose transport of Salmonella typhimurium has been reconstituted in proteoliposomes made from a partially purified protein fraction (containing the three membrane protein implicated in this transport and a lipoamide dehydrogenase activity) and soybean phospholipids. The reconstitution of galactose transport requires the addition of the purified galactose binding protein. Transport is energized either by reduced lipoamide and NAD or by the membrane potential and is inhibited by ATP.
D000255:Adenosine Triphosphate / Q000494:pharmacology*; D002135:Calcium-Binding Proteins; D002352:Carrier Proteins / Q000378:metabolism*; D002462:Cell Membrane / Q000187:drug effects / Q000502:physiology; D005690:Galactose / Q000378:metabolism*; D007700:Kinetics; D008081:Liposomes; D008564:Membrane Potentials; D009004:Monosaccharide Transport Proteins; D009243:NAD / Q000494:pharmacology*; D033902:Periplasmic Binding Proteins; D011510:Proteolipids / Q000378:metabolism*; D012486:Salmonella typhimurium / Q000378:metabolism*; D008063:Thioctic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology
D002135:Calcium-Binding Proteins; D002352:Carrier Proteins; D008081:Liposomes; D009004:Monosaccharide Transport Proteins; D033902:Periplasmic Binding Proteins; D011510:Proteolipids; C023550:galactose-binding protein; C020485:proteoliposomes; D009243:NAD; D008063:Thioctic Acid; D000255:Adenosine Triphosphate; C013091:lipoamide; D005690:Galactose
Reconstitution du transport de haute affinité du galactose de Salmonella typhimurium dans des protéoliposomes: énergisation par le lipoamide et le NAD ou par le potentiel de membrane; inhibition par l'ATP.