Target cell recognition structures in LDCC and ODCC.
Cytotoxic T lymphocyte effector cells specific for a defined class I antigen can kill target cells displaying a wide range of different class I proteins in the presence of certain lectins and oxidizing agents. However, optimal lysis of the target cell (TC) still requires interaction of the CTL with the TC class I proteins. This raises the question of how the lectin or oxidizing agent alters the system in such a way that an "inappropriate" CTL-TC interaction takes place, in a class I-dependent manner. In this study we show that if papain-sensitive molecules are cleared from the TC surface and are allowed to regenerate in the presence of tunicamycin, the cells still serve as targets in direct, class I antigen-specific CTL killing, but not in LDCC or ODCC. Target cells treated in this way display N-linked carbohydrate-less class I proteins, and presumably other N-linked carbohydrate-less, papain-sensitive molecules as well. We present data showing that both types of molecules are important in nonspecific lytic reactions.
D000818:Animals; D000954:Antigens, Surface / Q000276:immunology; D002460:Cell Line; D003208:Concanavalin A / Q000378:metabolism / Q000494:pharmacology*; D003602:Cytotoxicity, Immunologic / Q000187:drug effects*; D006183:H-2 Antigens / Q000276:immunology; D012515:Mast-Cell Sarcoma; D051379:Mice; D008810:Mice, Inbred C57BL / Q000276:immunology; D008811:Mice, Inbred DBA / Q000276:immunology; D009439:Neuraminidase / Q000494:pharmacology; D010206:Papain / Q000494:pharmacology; D011952:Receptors, Concanavalin A / Q000187:drug effects; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D000954:Antigens, Surface; D006183:H-2 Antigens; D011952:Receptors, Concanavalin A; D003208:Concanavalin A; D009439:Neuraminidase; D010206:Papain