Salt tolerance of lactose-grown Vibrio parahaemolyticus carrying Escherichia coli lac genes.
Lac- strains of Vibrio parahaemolyticus were converted to Lac+ on receiving a hybrid plasmid containing the lactose utilization genes of Escherichia coli K-12. A V. parahaemolyticus strain containing this hybrid plasmid exhibited optimal growth rates on glucose and other carbon sources in the presence of 0.2 to 0.4 M NaCl. Growth of the same strain on lactose was inhibited at similar concentrations of NaCl. The altered growth rate responses in lactose medium appeared to be attributable to effects of NaCl on the activity of lactose permease, and possibly on that of beta-galactosidase, rather than on the levels of these enzymes in V. parahaemolyticus cells.
D003470:Culture Media; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000235:genetics; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial; D007763:Lac Operon; D007785:Lactose / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000378:metabolism; D009004:Monosaccharide Transport Proteins; D010957:Plasmids; D012965:Sodium Chloride / Q000494:pharmacology*; D027981:Symporters; D014169:Transformation, Bacterial; D014736:Vibrio parahaemolyticus / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D001616:beta-Galactosidase / Q000378:metabolism
D003470:Culture Media; D004274:DNA, Recombinant; D029968:Escherichia coli Proteins; C050221:LacY protein, E coli; D026901:Membrane Transport Proteins; D009004:Monosaccharide Transport Proteins; D027981:Symporters; D012965:Sodium Chloride; C021499:lactose permease; D001616:beta-Galactosidase; D007785:Lactose