A sensitive bridge heterologous enzyme immunoassay of progesterone using geometrical isomers.
A sensitive bridge heterologous enzyme immunoassay of progesterone using geometrical isomers of progesterone 3(O-carboxymethyl)oxime(E/Z) was developed. Isomers were separated by synthesis of N-hydroxysuccinimide esters. Progesterone 3(Z)(O-carboxymethyl)oxime N-hydroxysuccinimide ester bound with beta-galactosidase in an appropriate molar ratio provided a conjugate suitable for an enzyme immunoassay. The antiserum was raised in rabbits by immunizing the animals with the progesterone 3(E)(O-carboxymethyl)oxime-bovine serum albumin conjugate. This bridge heterologous enzyme immunoassay proved to have sufficient sensitivity equivalent to radioimmunoassay and excellent specificity.
D000941:Antigens; D005260:Female; D006801:Humans; D007106:Immune Sera; D007124:Immunoenzyme Techniques; D007202:Indicators and Reagents; D007536:Isomerism; D011374:Progesterone / Q000097:blood*; D001616:beta-Galactosidase
D000941:Antigens; D007106:Immune Sera; D007202:Indicators and Reagents; D011374:Progesterone; D001616:beta-Galactosidase