Interaction of bovine erythrocyte N-glycolylneuraminic acid-containing gangliosides and glycoproteins with a human Hanganutziu-Deicher serum.
A human Hanganutziu-Deicher (H-D) serum reacted with N-glycolylneuraminic acid (NeuGc)-containing gangliosides and glycoproteins isolated from bovine erythrocyte membranes. Three populations of H-D antibodies were identified in the human H-D serum. One population very likely recognized the NeuGc-Gal sequence; a second population appears to recognize additional sugars in the oligosaccharide sequence, e.g. NeuGc-Gal-GlcNAc; while a third population may also recognize polypeptide determinants in addition to the NeuGc-Gal-GlcNAc sequence. The H-D serum distinguished two high mol. wt glycoproteins (HMGP I and II) present in crude extracts of bovine erythrocyte membranes. These glycoproteins were separated by repetitive fractionation on Sephacryl S-1000 in the presence of urea and their composition determined.
D000818:Animals; D000910:Antibodies, Heterophile / Q000032:analysis*; D002417:Cattle; D002850:Chromatography, Gel; D004910:Erythrocyte Membrane / Q000276:immunology*; D005732:Gangliosides / Q000276:immunology*; D006023:Glycoproteins / Q000276:immunology*; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D005779:Immunodiffusion; D007123:Immunoelectrophoresis, Two-Dimensional; D009438:Neuraminic Acids / Q000276:immunology*
D000910:Antibodies, Heterophile; D005732:Gangliosides; D006023:Glycoproteins; D009438:Neuraminic Acids; C032592:N-glycolylneuraminic acid