Thyrotropin-releasing hormone (TRH) degradation in NIH:N mouse cerebral cortex and spinal cord.
Thyrotropin-releasing hormone (TRH) metabolism in NIH:N mouse cerebral cortex and spinal cord was studied with high pressure liquid chromatography. Degradation of added TRH was significantly (P less than 0.001) slower in the spinal cord (2.19 +/- 0.46%/min) than in the cerebral cortex (7.54 +/- 0.31%/min). Bacitracin, a non-competitive inhibitor of TRH deamidase, inhibited TRH degradation by 61.3 +/- 3.7% in the spinal cord but only by 35.9 +/- 1.0% in the cerebral cortex (P less than 0.001). Catabolism of TRH in the mouse spinal cord proceeds at a slower rate and predominantly via the TRH deamidase pathway.
D000581:Amidohydrolases / Q000378:metabolism; D000818:Animals; D001414:Bacitracin / Q000494:pharmacology; D002540:Cerebral Cortex / Q000378:metabolism*; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D051379:Mice; D009928:Organ Specificity; D013116:Spinal Cord / Q000378:metabolism*; D013973:Thyrotropin-Releasing Hormone / Q000378:metabolism*
D001414:Bacitracin; D013973:Thyrotropin-Releasing Hormone; D000581:Amidohydrolases; C019579:TRH deamidase