title: Substrate recognition by vitamin K-dependent carboxylase.
abstract: Decarboxylated osteocalcins were prepared and purified from bovine, chicken, human and monkey bones and assayed for their ability to serve as a substrate for vitamin K-dependent carboxylase from bovine liver. Substantial differences were observed, especially between bovine and monkey d-osteocalcin. Since these substrates differ only in their amino acid residues 3 and 4, it seems that these residues play a role in the recognition of a substrate by hepatic carboxylase.
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002135:Calcium-Binding Proteins / Q000302:isolation & purification; D019735:Carbon-Carbon Ligases; D002417:Cattle; D002645:Chickens; D003653:Decarboxylation; D000882:Haplorhini; D006801:Humans; D008025:Ligases / Q000302:isolation & purification / Q000502:physiology*; D008099:Liver / Q000201:enzymology; D015675:Osteocalcin; D013045:Species Specificity; D013379:Substrate Specificity
chemical_list: D002135:Calcium-Binding Proteins; D015675:Osteocalcin; D008025:Ligases; D019735:Carbon-Carbon Ligases; C020660:glutamyl carboxylase