[Determination of beta-galactosidase activity on nitrocellulose plates using 5-bromo-3-indolyl-beta-D-galactopyranoside and tetrazolium salts].
A simple and convenient technique has been developed for detection of beta-galactosidase from E. coli on nitrocellulose sheets using a mixture of 5-bromoindol-3-yl-beta-D-galactopyranoside and nitro blue tetrazolium, which enables rapid detection of fmole (10(-15) mole) quantities of the enzyme at pH 9.5. The technique has the following advantages: the substrates are stable for a long period; reaction products give non-fading intense blue colour, resolution is extremely good with essentially no diffusion.
D055598:Chemical Phenomena; D002621:Chemistry; D003101:Collodion; D005696:Galactosidases / Q000032:analysis*; D005697:Galactosides; D006027:Glycosides; D007202:Indicators and Reagents; D007211:Indoles; D013379:Substrate Specificity; D013778:Tetrazolium Salts; D001616:beta-Galactosidase / Q000032:analysis*
D005697:Galactosides; D006027:Glycosides; D007202:Indicators and Reagents; D007211:Indoles; D013778:Tetrazolium Salts; D003101:Collodion; C052461:5-bromo-3-indolyl-beta-galactoside; D005696:Galactosidases; D001616:beta-Galactosidase
Opredelenie beta-galaktozidaznoĭ aktivnosti na nitrotselliuloznykh plastinakh s pomoshch'iu 5-brom-3-indolil-beta-D-galaktopiranozida v kombinatsii s soliami tetrazoliia.