Inhibition by pertussis toxin of fibroblast growth factor-stimulated hexose transport in Swiss 3T3 cells.
Addition of fibroblast growth factor to quiescent cultures of Swiss 3T3 cells stimulated the membrane transport of 2-deoxyglucose. Treatment of the cells with pertussis toxin (islet-activating protein) inhibited fibroblast growth factor-stimulated hexose transport. 5'-Guanylyl imidodiphosphate (p[NH]ppG), a non hydrolyzable analogue of GTP, increased the number of hexose carriers in the plasma membrane of saponin-permeabilized cells. These results suggest that guanine nucleotide binding protein may be involved in the regulation of hexose transport system by fibroblast growth factor in Swiss 3T3 cells.
D000345:Affinity Labels; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D000001:Calcimycin / Q000494:pharmacology; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D003571:Cytochalasin B; D003847:Deoxyglucose / Q000378:metabolism; D004533:Egtazic Acid / Q000494:pharmacology; D005346:Fibroblast Growth Factors / Q000494:pharmacology*; D019204:GTP-Binding Proteins / Q000502:physiology; D006165:Guanylyl Imidodiphosphate / Q000494:pharmacology; D006601:Hexoses / Q000378:metabolism*; D007700:Kinetics; D009004:Monosaccharide Transport Proteins / Q000378:metabolism; D037342:Pertussis Toxin; D010777:Photochemistry; D010566:Virulence Factors, Bordetella / Q000494:pharmacology*
D000345:Affinity Labels; D006601:Hexoses; D009004:Monosaccharide Transport Proteins; D010566:Virulence Factors, Bordetella; D006165:Guanylyl Imidodiphosphate; D000001:Calcimycin; D003571:Cytochalasin B; D004533:Egtazic Acid; D005346:Fibroblast Growth Factors; D003847:Deoxyglucose; D037342:Pertussis Toxin; D019204:GTP-Binding Proteins