Characterization of a precursor T-cell line (THP-6) with rearranged T-cell receptor beta chain gene.
A previously established human leukemia cell line, designated THP-6, was further characterized with respect to cell surface antigen expression and immunoglobulin(Ig) and T-cell receptor(TCR) gene status. THP-6 cells were positive for CD7 and CD5 antigens and terminal deoxynucleotidyl transferase, but negative for CD2, CD1, CD4, CD8, CD10, cytoplasmic and surface CD3 and HLA-DR antigens, suggesting a precursor T-cell line. Analysis of Ig and TCR beta chain genes revealed that THP-6 had a rearranged TCR beta chain gene and a germline Ig gene. These results, in agreement with its phenotype, confirmed that THP-6 was of the T-cell lineage.
D002460:Cell Line; D002648:Child; D003470:Culture Media / Q000494:pharmacology; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D005260:Female; D005803:Genes, Immunoglobulin; D006412:Hematopoietic Stem Cells / Q000145:classification / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D010641:Phenotype / Q000187:drug effects; D010835:Phytohemagglutinins / Q000494:pharmacology; D054218:Precursor T-Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*; D013601:T-Lymphocytes / Q000145:classification / Q000187:drug effects / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D003470:Culture Media; D007143:Immunoglobulin Heavy Chains; D010835:Phytohemagglutinins; D011948:Receptors, Antigen, T-Cell; D013755:Tetradecanoylphorbol Acetate; D004121:Dimethyl Sulfoxide