title: Mutagenicity and nitropyrene concentration of indoor air particulates exhausted from a kerosene heater.
abstract: The particulates in a room warmed with a radiant kerosene heater were collected, extracted and fractionated into diethyl ether-soluble neutral, acidic and basic fractions. The mutagenicity of these fractions was measured with Salmonella typhimurium strains TA98, TA98NR, TA98/1,8-DNP6 and TA100 in the presence and absence of S9 mix. Room air without the heater showed very low mutagenicity. However, a sample from a room at the beginning of the burning period showed very high mutagenicity (237 His+ revertants/plate/m3 of air in strain TA98 in the absence of S9 mix). In contrast, emissions from the heater after it was burning stably showed low mutagenicity (9 His+ revertants/plate/m3). The crude extract of particulates from the heater at the beginning of the burning period was analyzed by high-pressure liquid chromatography (HPLC) and showed a considerable amount of nitropyrenes (NPs); the concentrations of 1-NP and 1,6-diNP were 1.62 ng and 0.149 ng/m3 of air, respectively, and accounted for 1.2% and 17.6%, respectively, of the mutagenicity in strain TA98 in the absence of S9 mix. In addition, an HPLC-Ames histogram showed that peaks of mutagenicity corresponding to 1-NP and diNPs accounted for 75.7% (1-NP, 4.9%; 1,6-diNP, 17.1%; 1,8-diNP, 46.3%; 1,3-diNP, 7.4%) of the HPLC-recovered mutagenicity for strain TA98 without S9 mix. These results that kerosene heaters, especially immediately after ignition, create mutagenic substances such as NPs.
mesh_terms: D000393:Air Pollutants / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D006361:Heating; D007648:Kerosene; D009153:Mutagens / Q000032:analysis*; D009585:Nitrogen Dioxide / Q000032:analysis; D010578:Petroleum; D011721:Pyrenes / Q000032:analysis*
chemical_list: D000393:Air Pollutants; D007648:Kerosene; D009153:Mutagens; D010578:Petroleum; D011721:Pyrenes; C033309:1,6-dinitropyrene; D009585:Nitrogen Dioxide; C032668:1-nitropyrene