Evaluation of a fluorescent monoclonal antibody reagent for identification of cultured Neisseria gonorrhoeae.
We evaluated a new fluorescent monoclonal antibody reagent for confirmation of identity of Neisseria gonorrhoeae. The reagent correctly identified all 161 fresh clinical isolates of N. gonorrhoeae, which included 11 penicillinase producing strains (PPNG). The reagent also correctly identified 21 stored PPNG strains. No cross reactions were seen with 58 fresh clinical isolates of N. meningitidis, 12 stored strains of N. lactamica, or with strains of Gardnerella vaginalis, lactobacilli, Candida spp., Staphylococcus epidermidis or Enterobacteriaceae. Some cross reaction was noted with strains of S. aureus, probably related to cell-wall protein. A. However, this reagent was highly sensitive and specific for use against oxidase positive, gram-negative cocci isolated in London.
D000911:Antibodies, Monoclonal / Q000276:immunology; D003429:Cross Reactions; D005260:Female; D005455:Fluorescent Antibody Technique; D006194:Gardnerella vaginalis / Q000276:immunology / Q000302:isolation & purification; D006801:Humans; D008297:Male; D009343:Neisseria / Q000276:immunology / Q000302:isolation & purification; D009344:Neisseria gonorrhoeae / Q000201:enzymology / Q000276:immunology / Q000302:isolation & purification*; D009345:Neisseria meningitidis / Q000276:immunology / Q000302:isolation & purification; D010405:Penicillinase / Q000096:biosynthesis; D011237:Predictive Value of Tests; D013211:Staphylococcus aureus / Q000276:immunology / Q000302:isolation & purification
D000911:Antibodies, Monoclonal; D010405:Penicillinase