title: Endothelial injury and pulmonary congestion characterize neurogenic pulmonary edema in rabbits.
abstract: The objectives of the present study were to determine whether an intracisternal injection of fibrinogen-sodium citrate, a model of neurogenic pulmonary edema (NPE), produces protein-rich or protein-poor pulmonary edema, and to determine whether the edema is associated with pulmonary vascular hypertension and pulmonary congestion. Fibrinogen (6-10 mg/ml) dissolved in 0.055 M sodium citrate was injected into the cisterna magna of six New Zealand White rabbits. Six additional rabbits were injected with saline to control for the effects of intracranial hypertension and pulmonary vascular hypertension. The fibrinogen-sodium citrate solution or sodium citrate alone, as opposed to saline, produced systemic and pulmonary vascular hypertension, pulmonary edema, hypoxemia, hypercapnia, and acidosis. The lungs from fibrinogen-injected rabbits were edematous, congested, and liverlike in appearance. Tracheal froth that was blood tinged and protein rich was present in five of the six rabbits. Microscopic examination of lung biopsies revealed erythrocytes and plasma in the alveoli and focal injury to the pulmonary microvascular endothelium. Fibrinogen-sodium citrate increased (P less than 0.05) the extravascular lung water (EVLW) (10.3 +/- 2.0 vs. 5.5 +/- 0.6 g, means +/- SE), lung blood weight (9.7 +/- 1.3 vs. 3.8 +/- 0.6 g), total dry lung weight (3.2 +/- 0.4 vs. 2.0 +/- 0.1 g), and the EVLW-to-blood-free dry lung weight ratio (7.0 +/- 0.8 vs. 4.0 +/- 0.3 g) from saline-control values. There was no difference in the blood-fre dry lung weight (1.4 +/- 0.1 vs. 1.3 +/- 0.1 g) between the two groups. These findings demonstrate that pulmonary congestion, pulmonary vascular hypertension, and focal endothelial injury contribute to the development of NPE.
mesh_terms: D000818:Animals; D001639:Bicarbonates / Q000097:blood; D001794:Blood Pressure; D001927:Brain Diseases / Q000473:pathology* / Q000503:physiopathology; D002245:Carbon Dioxide / Q000097:blood; D004727:Endothelium / Q000473:pathology*; D007958:Leukocyte Count; D008168:Lung / Q000033:anatomy & histology / Q000473:pathology* / Q000648:ultrastructure; D008854:Microscopy, Electron; D009929:Organ Size; D010100:Oxygen / Q000097:blood; D010976:Platelet Count; D011654:Pulmonary Edema / Q000209:etiology / Q000473:pathology* / Q000503:physiopathology; D011817:Rabbits
chemical_list: D001639:Bicarbonates; D002245:Carbon Dioxide; D010100:Oxygen