title: An ELISA using an SDS extract of Brucella abortus strain 99 as antigen to detect B. abortus antibodies in cattle sera.
abstract: An ELISA was developed using an SDS extract of Brucella abortus as antigen to detect antibodies in cattle sera. The antigen was stable at 4 degrees C for at least 4 years and although it gave optimal results at a 1/4,000 dilution it could detect reactors at a 1/32,000 dilution. Based on comparative CF tests on 430 sera from negative herds and 187 sera from positive herds a reading of 0,07 or less using a 492 nm filter could be considered a negative reaction. The ELISA could be a useful test to supplement the CF test but further evaluation is still required.
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis; D000942:Antigens, Bacterial / Q000276:immunology*; D002003:Brucella abortus / Q000276:immunology*; D002007:Brucellosis, Bovine / Q000175:diagnosis* / Q000276:immunology; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D012967:Sodium Dodecyl Sulfate
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D012967:Sodium Dodecyl Sulfate