Fractionation of synaptophysin-containing vesicles from rat brain and cultured PC12 pheochromocytoma cells.
Synaptophysin is a transmembrane glycoprotein of neuroendocrine vesicles. Its content and distribution in subcellular fractions from cultured PC12 cells, rat brain and bovine adrenal medulla were determined by a sensitive dot immunoassay. Synaptophysin-containing fractions appeared as monodispersed populations similar to synaptic vesicles in density and size distribution. Membranes from synaptic vesicles contained approximately 100-times more synaptophysin than chromaffin granules. In conclusion, synaptophysin is located almost exclusively in vesicles of brain and PC12 cells which are distinct from dense core granules.
D000313:Adrenal Medulla / Q000032:analysis; D000818:Animals; D001923:Brain Chemistry; D002417:Cattle; D002499:Centrifugation, Density Gradient; D007163:Immunosorbent Techniques; D008565:Membrane Proteins / Q000302:isolation & purification*; D008970:Molecular Weight; D010673:Pheochromocytoma / Q000032:analysis*; D051381:Rats; D013572:Synaptic Vesicles / Q000032:analysis*; D016708:Synaptophysin; D013574:Synaptosomes / Q000032:analysis; D014407:Tumor Cells, Cultured
D008565:Membrane Proteins; D016708:Synaptophysin