[Testing of acute myeloid leukemia of humans using monoclonal antibodies BL-DR, BL-M/G, BL-T2 and BL-Ig-L/1].
The leukemic blasts of 22 patients with acute myelocytic and myelomonocytic leukemia were tested by indirect immunofluorescence with the monoclonal antibodies BL-DR (directed against HLA-DR-antigens), BL-M/G (react with both granulocytes and monocytes), BL-T2 (pan-T-lymphocyte-antibody CD 5), and BL-Ig-L/1 (anti-light-chains-antibody). The leukemic blasts showed no crossreaction with BL-DR and BL-Ig-L/1. Both the antibodies BL-DR and BL-M/G reacted mainly with the acute myelomonocytic leukemias FAB M4 and only seldom with the acute myelocytic leukemias FAB M1, M2, and M3. The antibodies BL-DR and BL-M/G are able to confirm the diagnosis of acute myelomonocytic leukemia and to classify some previously unclassifiable leukemias.
D000911:Antibodies, Monoclonal; D001402:B-Lymphocytes / Q000276:immunology; D003429:Cross Reactions; D005455:Fluorescent Antibody Technique; D006098:Granulocytes / Q000276:immunology; D006684:HLA-DR Antigens / Q000276:immunology; D006801:Humans; D007147:Immunoglobulin Light Chains / Q000276:immunology; D015470:Leukemia, Myeloid, Acute / Q000145:classification / Q000175:diagnosis*; D009000:Monocytes / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology
D000911:Antibodies, Monoclonal; D006684:HLA-DR Antigens; D007147:Immunoglobulin Light Chains
Testung von akuten myeloischen Leukämien des Menschen mittels der monoklonalen Antikörper BL-DR, BL-M/G, BL-T2 und BL-Ig-L/1.