Rapid isolation of human kininogens.
A rapid, two-step procedure is described for the isolation of both "high molecular weight" (H-) and "low molecular weight" (L-) plasma kininogens from a single sample of plasma. Affinity chromatography on carboxymethyl-papain-Sepharose is used, together with high-resolution anion exchange chromatography.
D001777:Blood Coagulation / Q000187:drug effects; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D007704:Kininogens / Q000302:isolation & purification* / Q000494:pharmacology; D008970:Molecular Weight; D010206:Papain / Q000302:isolation & purification; D012967:Sodium Dodecyl Sulfate
D007704:Kininogens; D012967:Sodium Dodecyl Sulfate; D010206:Papain