title: Neurofilament degradation by bovine brain cathepsin D.
abstract: The effect of cathepsin D on bovine neurofilament protein was studied biochemically, immunologically, and morphologically. Degradation products of each neurofilament triplet by bovine brain cathepsin D at neutral pH were identified by electrophoresis and immunoblotting with anti-neurofilament antibodies. The 68-kDa subunit was the most susceptive to cathepsin D proteolysis among the triplet proteins. All of the triplet gave rise to partial degradates of the 50-kDa size. The reconstituted fiber from neurofilament triplet proteins and the 68-kDa subunit protein were attacked by cathepsin D and the mode of disruption of the fiber structure was studied by electronmicroscopy.
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D002402:Cathepsin D / Q000494:pharmacology*; D002417:Cattle; D007381:Intermediate Filament Proteins / Q000378:metabolism*; D016900:Neurofilament Proteins; D013116:Spinal Cord / Q000187:drug effects / Q000378:metabolism
chemical_list: D007381:Intermediate Filament Proteins; D016900:Neurofilament Proteins; D002402:Cathepsin D