Partial purification and biological activity of the enterotoxic component of Pseudomonas aeruginosa.
From an Pseudomonas aeruginosa strain isolated from feces of a patient with clinical manifestation of diarrhoeal disease a filtrate from the culture in proteose peptone broth was prepared. After concentration, the culture filtrate was separated on Sephadex G-100 column in four fractions. The first high molecular fraction (F1) represented components of relative molecular mass exceeding 100,000, the second fraction those about 58,000, the third fraction those about 23,000 and the fourth one was composed from low molecular components of molecular mass lower than 10,000. In biological tests, the most marked reactions were caused by the third fraction. It caused a fluid accumulation in the rabbit ileal loop test, reactions in the rapid and delayed skin tests and in the mouse foot edema test. Besides these activities the third fraction caused also a hemolytic, proteolytic and elastase activity. A clear cytotoxic effect of this fraction was seen on Vero, Y1 and CHO cell lines. It was also found, that after heating (100 degrees C/10 min) both, the enterotoxic and hemolytic activities were lost.
D000818:Animals; D001431:Bacteriological Techniques; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D003967:Diarrhea / Q000382:microbiology*; D004768:Enterotoxins / Q000302:isolation & purification*; D005243:Feces / Q000382:microbiology; D006801:Humans; D051379:Mice; D008970:Molecular Weight; D011552:Pseudomonas Infections / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000032:analysis*
D004768:Enterotoxins