Visualization of anti-I-J antibody binding sites on bone marrow-derived macrophages.
I-J antigens on BMDM were visualized by immunofluorescence. Nonspecific binding of IgG antibody to Fc receptors was eliminated by the pretreatment of the antibody with anti-mouse IgG Fc portion antibody.
D000818:Animals; D001665:Binding Sites; D001667:Binding, Competitive; D001853:Bone Marrow / Q000276:immunology; D005455:Fluorescent Antibody Technique; D000949:Histocompatibility Antigens Class II / Q000302:isolation & purification*; D007518:Isoantibodies; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL
D000949:Histocompatibility Antigens Class II; C043911:I-J-antigen; D007518:Isoantibodies