Isolation by Percoll gradient centrifugation and radiolabeling of bloodstream forms of Trypanosoma cruzi.
A method to isolate T. cruzi bloodstream forms was designed taking advantage of Percoll self-generated gradients and isopycnic centrifugation, resulting in a good resolution between parasites and host cells. Purified parasites incorporated 3H-uridine giving values of 23% precipitable by TCA. Viability of the parasites was totally preserved as evidenced by the "in vivo" infectivity assays.
D000818:Animals; D002469:Cell Separation; D002499:Centrifugation, Density Gradient / Q000379:methods*; D002500:Centrifugation, Isopycnic; D014355:Chagas Disease / Q000097:blood*; D004912:Erythrocytes; D014349:Trypanosoma cruzi / Q000302:isolation & purification*