Post-transcriptional regulation and DNA undermethylation of intracisternal A particle genes in embryonal carcinoma cell lines.
Northern blot analysis and in vitro nuclear transcription assays were performed in order to clarify conflicting reports on the expression of intracisternal A particle (IAP) genes in embryonal carcinoma (EC) cell lines. Results demonstrate that post-transcriptional mechanisms control the final steady-state levels of IAP RNA in EC cells. IAP genes were further found to be undermethylated in IAP-expressing EC cell lines.
D000818:Animals; D004273:DNA, Neoplasm / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation; D007426:Genes, Intracisternal A-Particle; D008745:Methylation; D011519:Proto-Oncogenes; D012323:RNA Processing, Post-Transcriptional; D012334:RNA, Neoplasm / Q000235:genetics / Q000378:metabolism; D013724:Teratoma / Q000235:genetics* / Q000378:metabolism; D014407:Tumor Cells, Cultured / Q000378:metabolism
D004273:DNA, Neoplasm; D012334:RNA, Neoplasm