In vivo cytotoxicity assays with 111In-labeled tumor cells: comparison with [125I]iododeoxyuridine-labeled cells and effects of chelators and inoculation site.
The fate of cells inoculated in nude mice can be determined using human tumor cells prelabeled with 111In oxine (111InOx). To facilitate elimination of extracellular 111In released from killed cells, an appropriate metal chelating agent is administered to the host mice. With calcium disodium edetate administered by various routes, highly significant differences in the rates of 111In loss were observed for viable compared to killed cells after i.p. or i.m. inoculation. Radioactivity was eliminated at similar rates for mice bearing viable cells labeled with 111In or with [125I]iododeoxyuridine (125IUdR). For killed cells, 111In was eliminated somewhat less efficiently than 125I. The 111In-prelabeling method allows facile and sensitive determination of host-mediated cytotoxicity for inoculated tumor cells.
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D003602:Cytotoxicity, Immunologic; D004492:Edetic Acid / Q000494:pharmacology*; D005260:Female; D006801:Humans; D007205:Indium Radioisotopes; D007457:Iodine Radioisotopes; D008545:Melanoma / Q000000981:diagnostic imaging* / Q000276:immunology / Q000473:pathology; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D011877:Radionuclide Imaging; D014183:Transplantation, Heterologous
D007205:Indium Radioisotopes; D007457:Iodine Radioisotopes; D004492:Edetic Acid