Immunoglobulin E mediated membrane conductance changes in rat basophilic leukemia cells.
Electrophysiological effects of anaphylactic stimulation of rat basophilic leukemia cells (RBL-2H3) were studied using conventional microelectrodes. Stimulation of passively sensitized cells by anti-immunoglobulin E resulted in hyperpolarization followed by depolarization. These changes in membrane polarization were associated with a decrease in input membrane resistance. No effect of anaphylactic stimulation was seen in Ca2+-free solution or when Ca2+ influx was blocked by Co2+, but it was mimicked by the Ca2+ ionophore A-23187. This suggests that the changes in ionic conductances were associated with calcium influx. These results support the hypothesis that membrane conductance changes are involved in the stimulus-secretion process of the RBL-2H3 cells.
D000200:Action Potentials / Q000187:drug effects; D000707:Anaphylaxis / Q000503:physiopathology*; D000818:Animals; D000937:Antigen-Antibody Reactions; D001491:Basophils; D000001:Calcimycin / Q000494:pharmacology; D002461:Cell Line, Transformed; D002462:Cell Membrane / Q000187:drug effects / Q000502:physiology; D007073:Immunoglobulin E / Q000502:physiology*; D007942:Leukemia, Experimental / Q000503:physiopathology*; D008839:Microelectrodes; D051381:Rats
D007073:Immunoglobulin E; D000001:Calcimycin