Effects of sodium aurothiomalate on the polyclonal immunoglobulin secretion by human mononuclear cells in vitro.
Sodium aurothiomalate (GSTM) inhibited the pokeweed mitogen induced plaque forming cell (PFC) response in a dose dependent manner. This inhibition was irreversible as preincubation for 2 h with the drug followed by washes still caused a suppression of the PFC response. Pretreatment of the different mononuclear cell populations necessary for the PFC response with GSTM for short periods (2-24 h) showed that both macrophages (M phi) and B lymphocytes were inhibited by the drug but that T cells were resistant. In the case of GSTM treated M phi the response was restored partially with exogenous interleukin-1, and completely with the addition of 20% fresh M phi. Our results show that the inhibition of polyclonal immunoglobulin secretion by human mononuclear cells in vitro is due to an effect of GSTM on both M phi and B lymphocytes. This may explain the decrease in serum immunoglobulin levels seen in patients receiving chrysotherapy.
D001402:B-Lymphocytes / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects; D002469:Cell Separation; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004351:Drug Resistance; D006052:Gold Sodium Thiomalate / Q000494:pharmacology*; D006801:Humans; D007136:Immunoglobulins / Q000378:metabolism*; D007375:Interleukin-1 / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects; D009000:Monocytes / Q000378:metabolism*; D013601:T-Lymphocytes / Q000187:drug effects
D007136:Immunoglobulins; D007375:Interleukin-1; D006052:Gold Sodium Thiomalate