Equivalence of UDS responses for established cell lines and primary cells derived from the mei-9a and mus201D1 excision repair-deficient strains of Drosophila melanogaster.
Autoradiographic analysis of unscheduled DNA synthesis in response to DNA damage produced by relatively high doses of the direct-acting, monofunctional alkylating agent, MMS, (4.5 mM) and UV (40 J/m2) demonstrates that established cell lines, derived from the mei-9a and mus201D1 excision repair-deficient strains of Drosophila melanogaster, perform no measurable incorporation of [3H]thymidine into repair patches, in accordance with the observations made for the corresponding primary embryonic cells derived from the two mutagen-sensitive strains of flies. These established cell lines can therefore be used as appropriate models for both the examination of the biochemical basis of the genetic defects in the mei-9 and mus201 mutations and the role of excision-repair processes in spontaneous and induced mutation induction in eukaryotic cells.
D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D004247:DNA / Q000187:drug effects / Q000528:radiation effects; D004249:DNA Damage; D004260:DNA Repair; D004331:Drosophila melanogaster / Q000235:genetics*; D008741:Methyl Methanesulfonate; D014466:Ultraviolet Rays
D004247:DNA; D008741:Methyl Methanesulfonate