Purification and characterization of rat-liver 3,4-dihydroxyphenylalanine decarboxylase.
A simple and rapid procedure, which takes advantage of the effectiveness of conventional and HPLC hydrophobic interaction, for the isolation of highly purified rat liver 3,4-dihydroxyphenylalanine decarboxylase is described in detail. Some of its structural and functional properties are reported and discussed in comparison with those of pig kidney 3,4-dihydroxyphenylalanine decarboxylase.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001142:Aromatic-L-Amino-Acid Decarboxylases / Q000302:isolation & purification*; D002845:Chromatography; D002851:Chromatography, High Pressure Liquid; D004296:Dopa Decarboxylase / Q000302:isolation & purification* / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005779:Immunodiffusion; D007668:Kidney / Q000201:enzymology; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008970:Molecular Weight; D011732:Pyridoxal Phosphate / Q000032:analysis; D051381:Rats; D013053:Spectrophotometry; D013379:Substrate Specificity; D013552:Swine
D000596:Amino Acids; D011732:Pyridoxal Phosphate; D004296:Dopa Decarboxylase; D001142:Aromatic-L-Amino-Acid Decarboxylases