Rapid identification of Branhamella catarrhalis with 4-methylumbelliferyl butyrate.
Branhamella catarrhalis can be distinguished from Neisseria spp. by the presence of butyrate esterase. This enzyme can be rapidly detected when 4-methylumbelliferyl butyrate is used as the substrate. All B. catarrhalis strains tested gave a positive fluorescence reaction within 5 min, while Neisseria spp. remained negative, even after 18 h of incubation.
D002265:Carboxylic Ester Hydrolases / Q000032:analysis*; D006923:Hymecromone / Q000031:analogs & derivatives*; D001936:Moraxella catarrhalis / Q000201:enzymology / Q000302:isolation & purification*; D009343:Neisseria / Q000201:enzymology; D014468:Umbelliferones
D014468:Umbelliferones; C056366:4-methylumbelliferyl butyrate; D006923:Hymecromone; D002265:Carboxylic Ester Hydrolases; C021487:butyrylesterase