Immobilization of linamarase and its use in the determination of bound cyanide in cassava using flow injection analysis.
Extracts from the tubers (cortex and parenchyma) and leaves of Manihot esculenta Crantz (cassava) were analyzed for their releasable cyanide content using flow injection analysis incorporating an immobilized linamarase bioreactor. Linamarase was immobilized under very mild conditions to an activated 2-fluoro-N-methylpyridinium Fractogel support. The released cyanide, which was monitored spectrophotometrically at 525 nm using an alkaline picrate reagent, was found to be highest in the cortex and lowest in the parenchyma.
D002138:Calibration; D055598:Chemical Phenomena; D002621:Chemistry; D003486:Cyanides / Q000032:analysis*; D004795:Enzyme Stability; D004800:Enzymes, Immobilized; D005959:Glucosidases; D006863:Hydrogen-Ion Concentration; D002365:Manihot / Q000032:analysis*; D010936:Plant Extracts / Q000032:analysis; D010945:Plants, Edible / Q000032:analysis*; D013696:Temperature; D001617:beta-Glucosidase
D003486:Cyanides; D004800:Enzymes, Immobilized; D010936:Plant Extracts; D005959:Glucosidases; C021514:cyanogenic beta-glucosidase; D001617:beta-Glucosidase