title: Prostaglandin E analogue inhibition of intrinsic factor release.
abstract: In many mammalian species (including humans) the parietal cell secretes both acid and intrinsic factor (IF). The aim of this study was to examine the direct effect of prostaglandin E analogues on basal and stimulated IF release in isolated, enriched rabbit parietal cells. The effects of graded concentrations (10(-12) to 10(-6) M) of 16,16-dimethyl prostaglandin E2 (DMPGE2) and 16-methyl,16-hydroxyl prostaglandin E1 (misoprostol) on submaximal histamine-stimulated (10(-6) M) secretion were tested. Both analogues failed to alter basal release of IF or aminopyrine accumulation (indirect measure of acid secretion). Increasing concentrations of DMPGE2 resulted in a dose-dependent inhibition of IF release (22 +/- 8% decrease at 10(-9) M; p less than 0.05) and a maximal effective response at 10(-7) M (54 +/- 9%; p less than 0.005). Dose-dependent inhibition of IF secretion was also observed with increasing concentrations of misoprostol, with a 22 +/- 7% decrease at 10(-9) M (p less than 0.05) and maximal effective inhibition at 10(-6) M (47 +/- 8%; p less than 0.01). Misoprostol and DMPGE2 inhibition of acid secretion paralleled IF release. Prostaglandin analogues appear to block IF release potently in histamine-stimulated parietal cells.
mesh_terms: D000527:Alprostadil / Q000031:analogs & derivatives* / Q000494:pharmacology; D000818:Animals; D005744:Gastric Acid / Q000378:metabolism; D006632:Histamine / Q000494:pharmacology; D007437:Intrinsic Factor / Q000378:metabolism*; D016595:Misoprostol; D010295:Parietal Cells, Gastric / Q000187:drug effects*; D011459:Prostaglandins E, Synthetic / Q000494:pharmacology*; D011817:Rabbits
chemical_list: D011459:Prostaglandins E, Synthetic; D016595:Misoprostol; C017244:16,16-dimethylprostaglandin E; D006632:Histamine; D007437:Intrinsic Factor; D000527:Alprostadil