title: Nuclear DNA analysis of oral hyperplasia and dysplasia using image cytometry.
abstract: We investigated the value of image analysis in discriminating among oral white lesions with hyperplasia without dysplasia and oral white or white-and-red lesions with moderate or severe dysplasia. Normal oral epithelial tissue was used as a control. Image analysis was applied to 5-micron formalin-fixed sections stained with the azure A-Feulgen reaction for nuclear DNA. For 150-200 cells from each section, 5 nuclear variables were assessed: area, form factor, total stain, average stain and ellipticity. For each variable, 2 measurements were obtained, the mean and the interquartile range, and were used for stepwise discriminant analysis. Using this test, a model of 3 measurements with the most discriminating power was developed. When the jackknife classification test was applied to this model, we could discriminate with 81% accuracy between the 4 groups of tissue studied.
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000648:ultrastructure; D002467:Cell Nucleus / Q000648:ultrastructure*; D002471:Cell Transformation, Neoplastic / Q000648:ultrastructure; D003584:Cytological Techniques; D004247:DNA / Q000032:analysis*; D006801:Humans; D006965:Hyperplasia / Q000473:pathology; D009059:Mouth Diseases / Q000473:pathology*; D009061:Mouth Mucosa / Q000648:ultrastructure*; D009062:Mouth Neoplasms / Q000648:ultrastructure; D011230:Precancerous Conditions / Q000648:ultrastructure
chemical_list: D004247:DNA