Ultrastructural autoradiographic localization of exogenous arachidonic acid in cultured endothelial and smooth muscle cells.
The uptake and intracellular localization of exogenous arachidonic acid (AA) were investigated in cultured endothelial (EC) and smooth muscle cells (SMC) isolated from bovine aorta. The [14C]AA uptake was assessed biochemically and by light and electron microscopic autoradiography. The highest values of silver grain surface density were associated with the mitochondria, lysosomes, and the Golgi apparatus of the EC. The grain linear density was greater on the nuclear envelope than on plasmalemma. On SMC, the grain density was highest on lipid droplets whereas the linear densities of the nuclear envelope and plasmalemma were similar. The share of each subcellular compartment in the AA distribution was estimated as the percentage of the individual silver grain count out of the total cell-associated radioactivity. The results showed that cytoplasm (including endoplasmic reticulum, ribosomes, and small vesicles) made the main contribution followed by the nucleus and at lower values by other organelles. These subcompartments may represent the intracellular sites from which AA could be mobilized for prostanoid synthesis by EC and SMC.
D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000032:analysis* / Q000493:pharmacokinetics; D001345:Autoradiography; D002417:Cattle; D004730:Endothelium, Vascular / Q000032:analysis*; D008854:Microscopy, Electron; D009131:Muscle, Smooth, Vascular / Q000032:analysis*
D001095:Arachidonic Acids; D016718:Arachidonic Acid