[Modification of the phenoloxidase activity of human leukocytes by detergents, studied by histochemical phenoloxidase detection].
Using the histochemical detection for PO the in vitro effect of several detergents was tested. Dimethylsulfoxide, Triton X 100, Sodium desoxycholate, Tween 20, and Cetyltrimethyl ammonium bromide were investigated to check their effects upon the morphology and enzymatic activity of human leucocytes. Cetyltrimethyl ammonium bromide seems to be most suitable for the disintegration of cells. It does not inhibit the PO-activity.
D004156:Catechol Oxidase / Q000097:blood*; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000494:pharmacology; D003840:Deoxycholic Acid / Q000494:pharmacology; D003902:Detergents / Q000494:pharmacology*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D006651:Histocytochemistry; D006801:Humans; D007962:Leukocytes / Q000187:drug effects* / Q000201:enzymology; D014442:Monophenol Monooxygenase / Q000097:blood*; D017830:Octoxynol; D011092:Polyethylene Glycols / Q000494:pharmacology; D011136:Polysorbates / Q000494:pharmacology; D013501:Surface-Active Agents / Q000494:pharmacology*
D002593:Cetrimonium Compounds; D003902:Detergents; D011136:Polysorbates; D013501:Surface-Active Agents; D003840:Deoxycholic Acid; D011092:Polyethylene Glycols; D017830:Octoxynol; D004156:Catechol Oxidase; D014442:Monophenol Monooxygenase; D004121:Dimethyl Sulfoxide; D000077286:Cetrimonium
Beeinflussung der Phenoloxidaseaktivität menschlicher Leukocyten durch Detergentien, untersucht mittels des histochemischen Phenoloxidasenachweises.