Studies on DNA damage and repair in the mammalian brain.
Methods for studying breaks in DNA strands and their repair, originally developed for prokaryotes and cultured cell lines, have been applied to preparations from rat brain. The relative sensitivities of these methods, which include alkaline sucrose density gradient sedimentation, nucleoid sedimentation, and ADP-ribosyltransferase assay, are compared.
D000818:Animals; D001921:Brain / Q000378:metabolism* / Q000648:ultrastructure; D002458:Cell Fractionation; D002467:Cell Nucleus / Q000032:analysis / Q000648:ultrastructure; D002499:Centrifugation, Density Gradient; D004247:DNA / Q000528:radiation effects; D004249:DNA Damage; D004260:DNA Repair; D005260:Female; D005720:Gamma Rays; D007939:Leukemia L1210; D051379:Mice; D009457:Neuroglia / Q000648:ultrastructure; D009474:Neurons / Q000648:ultrastructure; D011065:Poly(ADP-ribose) Polymerases / Q000032:analysis; D051381:Rats; D014407:Tumor Cells, Cultured
D004247:DNA; D011065:Poly(ADP-ribose) Polymerases