A competitive enzyme-linked immunosorbent assay for alpha 1-acid glycoprotein in serum and urine samples.
In this solid-phase competitive enzyme-linked immunosorbent assay for alpha 1-acid glycoprotein in serum or urine, antiserum to human alpha 1-acid glycoprotein is incubated with solid-phase-bound alpha 1-acid glycoprotein in the presence of standard or sample. Incubation with second antibody labeled with alkaline phosphatase then follows, before development with substrate. Results obtained correlate well with a fluorescent assay involving the dye Auramine O (r = 0.953) and with radial immunodiffusion (r = 0.921). The present assay covers the range 0.2 to 5 mg/L and 16 samples take 2.5 h to complete. This assay is useful for measuring concentrations of alpha 1-acid glycoprotein in serum and also in urine, for which other assay methods are not sufficiently sensitive.
D001172:Arthritis, Rheumatoid / Q000097:blood; D001557:Benzenesulfonates; D001576:Benzophenoneidum; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D005779:Immunodiffusion; D008722:Methods; D009961:Orosomucoid / Q000097:blood* / Q000652:urine; D012459:Salicylates
D001557:Benzenesulfonates; D009961:Orosomucoid; D012459:Salicylates; D001576:Benzophenoneidum; C003366:sulfosalicylic acid