Platelet viability (aggregation, migration, recovery) after radiolabelling from hypercholesterolemics using various tracers (oxine, oxine-sulphate, tropolone, MPO).
Earlier results indicated a diminished labelling efficiency and recovery negatively linked to actual cholesterol and lipoprotein values in hyperlipoproteinemics. This study was designed to examine whether other alternative tracers exhibit similar results and to study the influence on platelet viability in the presence or absence of PGI2. We demonstrate that no substantial difference occurs between the four tracers concerning labelling efficiency and recovery in normo- and hypercholesterolemics. Cholesterol severely affects the labelling parameters for all the tracers to a comparable extent. The absolute platelet function varies considerably, however, the percent changes in normo- and hypercholesterolemics seen before and after the labelling procedure do not differ significantly. PGI2 improves recovery in general, however, without affecting labelling efficiency or in vitro viability testing. As prolonged incubation further increases labelling efficiency, the presence and extent of hyperlipoproteinemia should be known in order to avoid poor labeling and viability and subsequently poor clinical results.
D000328:Adult; D000368:Aged; D001792:Blood Platelets / Q000503:physiopathology*; D002465:Cell Movement; D005260:Female; D006801:Humans; D006937:Hypercholesterolemia / Q000097:blood*; D007205:Indium Radioisotopes; D007553:Isotope Labeling; D008297:Male; D008875:Middle Aged; D009942:Organometallic Compounds; D015125:Oxyquinoline / Q000031:analogs & derivatives; D010974:Platelet Aggregation; D011725:Pyridines; D013871:Thiones; D014334:Tropolone
D007205:Indium Radioisotopes; D009942:Organometallic Compounds; D011725:Pyridines; D013871:Thiones; C032950:indium oxine; D015125:Oxyquinoline; C008704:pyrithione; D014334:Tropolone