Identification of mouse J chain in human IgM produced by an electrofusion-derived mouse-human heterohybridoma.
EBV transformed human B cell line secreting IgM was fused with X63-Ag8.653 mouse cell line. Hybrids were selected in medium containing HAT and ouabain. IgM from a clone was purified by affinity chromatography. Western blot analysis of the heterohybridoma IgM showed the presence of mouse J chain when probed with antiserum against mouse J chain.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000096:biosynthesis; D001666:Binding Sites, Antibody; D002459:Cell Fusion / Q000379:methods; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D006825:Hybridomas / Q000276:immunology / Q000378:metabolism*; D015151:Immunoblotting; D007144:Immunoglobulin J-Chains / Q000096:biosynthesis* / Q000302:isolation & purification; D007075:Immunoglobulin M / Q000032:analysis / Q000096:biosynthesis*; D008070:Lipopolysaccharides / Q000378:metabolism; D051379:Mice
D000911:Antibodies, Monoclonal; D007144:Immunoglobulin J-Chains; D007075:Immunoglobulin M; D008070:Lipopolysaccharides