title: Carbon monoxide-dependent chemolithotrophic growth of Clostridium thermoautotrophicum.
abstract: The acetogen Clostridium thermoautotrophicum was cultivated under CO-dependent chemolithotrophic conditions. CO-dependent growth profiles and energetics indicated that supplemental CO2 was fundamental to efficient growth at the expense of CO. Overall product stoichiometry approximated 6.5CO----CH3CO2H + 3.5CO2 + 0.6 cell C + 0.5 unrecovered C. Initial CO/CO2 ratios of 2 to 4 yielded optimal doubling times and cell yields. Maximal YCO values approximated 2.5 g of cell dry weight per mol of CO consumed; YH2 was considerably lower than YCO. Cross-transfer growth experiments and protein profiles indicated differential expression of genes between CO and methanol cultures.
mesh_terms: D002245:Carbon Dioxide / Q000378:metabolism; D002248:Carbon Monoxide / Q000378:metabolism*; D003013:Clostridium / Q000254:growth & development* / Q000378:metabolism; D000432:Methanol / Q000378:metabolism
chemical_list: D002245:Carbon Dioxide; D002248:Carbon Monoxide; D000432:Methanol