Properties of 7-alkylguanosines as substrates of purine nucleoside phosphorylase.
A series of 7-alkyl analogues of guanosine was prepared by alkylation of 5'-GMP, and enzymatic dephosphorylation of the products to the corresponding nucleosides. The latter were all excellent, as well as fluorescent, substrates of calf spleen nucleoside phosphorylase. Kinetic parameters demonstrated that the purine ring N(7) is not a binding site for the enzyme.
D000478:Alkylation; D006151:Guanosine / Q000031:analogs & derivatives* / Q000138:chemical synthesis*; D007202:Indicators and Reagents; D007700:Kinetics; D010430:Pentosyltransferases / Q000378:metabolism*; D011683:Purine-Nucleoside Phosphorylase / Q000378:metabolism*; D013329:Structure-Activity Relationship; D013379:Substrate Specificity
D007202:Indicators and Reagents; D006151:Guanosine; D010430:Pentosyltransferases; D011683:Purine-Nucleoside Phosphorylase