The protein core of the largest proteoglycan monomer of articular cartilage. Gel electrophoretic pattern and tryptophanyl peptide bond cleavage.
The largest proteoglycan monomer of baboon (Papio papio) articular cartilage was isolated and the protein rich core was obtained after chondroitinase AC II and keratanase digestions. On SDS-PAGE the core yielded a single band with apparent Mr of 290,000. Tryptophanyl peptide bond cleavage of the core with N-chlorosuccinimide/urea gave 4 peptides with apparent Mr of 105,000, 66,000, 62,000 and 56,000
D000818:Animals; D002236:Carbohydrate Conformation; D002358:Cartilage, Articular / Q000032:analysis*; D002808:Chondroitin Lyases / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D006026:Glycoside Hydrolases; D006820:Hyaluronic Acid / Q000378:metabolism; D008970:Molecular Weight; D010215:Papio; D010455:Peptides / Q000378:metabolism*; D011509:Proteoglycans / Q000032:analysis* / Q000378:metabolism; D013388:Succinimides; D014364:Tryptophan / Q000032:analysis / Q000378:metabolism*; D014508:Urea; D001616:beta-Galactosidase / Q000378:metabolism
D010455:Peptides; D011509:Proteoglycans; D013388:Succinimides; C015752:N-chlorosuccinimide; D014364:Tryptophan; D014508:Urea; D006820:Hyaluronic Acid; D006026:Glycoside Hydrolases; C026093:keratan-sulfate endo-1,4-beta-galactosidase; D001616:beta-Galactosidase; D002808:Chondroitin Lyases