A modified conductance medium for the detection of Salmonella spp.
Selenite-cystine/trimethylamine oxide/dulcitol medium has been used in conjunction with conductance instruments to detect the presence of Salmonella spp. in foods and faeces. However, a small but significant number of salmonella strains were missed by this method. The majority of these strains were detected when dulcitol was substituted by mannitol and tested on two separate Malthus conductance instruments. Some strains of Citrobacter freundii and Escherichia coli continued to give false positive results. Attempts are made to explain why the substitution of mannitol for dulcitol gives an improved medium.
D003470:Culture Media; D004553:Electric Conductivity; D005189:False Positive Reactions; D005243:Feces / Q000382:microbiology*; D004376:Galactitol / Q000378:metabolism; D006801:Humans; D008353:Mannitol / Q000378:metabolism; D012475:Salmonella / Q000254:growth & development / Q000302:isolation & purification*
D003470:Culture Media; D004376:Galactitol; D008353:Mannitol