[The use of antibodies for differential determination of neutral and acid alpha-glucosidases in human urine in normal and pathological states].
Use of antibodies towards neutral alpha-glucosidase from human kidney brush border enabled to estimate distinctly the activity of acid alpha-glucosidase in urine of healthy persons and patients although the activity of neutral enzyme in urine exceeded markedly the acid enzyme activity. Simultaneous use of antibodies to both these enzymes permitted to estimate separately the activity of acid and neutral alpha-glucosidases in a mixture. Accuracy of these estimations was confirmed after consecutive use of these antibodies.
D004796:Clinical Enzyme Tests; D005921:Glomerulonephritis / Q000175:diagnosis; D005087:Glucan 1,4-alpha-Glucosidase / Q000276:immunology / Q000652:urine*; D005959:Glucosidases / Q000652:urine*; D006009:Glycogen Storage Disease Type II / Q000175:diagnosis; D006801:Humans; D007106:Immune Sera; D011233:Precipitin Tests; D000520:alpha-Glucosidases / Q000276:immunology / Q000652:urine*
D007106:Immune Sera; D005959:Glucosidases; D000520:alpha-Glucosidases; D005087:Glucan 1,4-alpha-Glucosidase
Ispol'zovanie antitel dlia differentsial'nogo opredeleniia neĭtral'noĭ i kisloĭ alpha-gliukozidaz mochi cheloveka v norme i pri patologii.