A study on heterogeneity in molecular species of shark cartilage chondroitin sulfate C. Fractionation of the polysaccharide on Sepharose CL-4B in the presence of high concentrations of ammonium sulfate.
Shark cartilage chondroitin sulfate C was fractionated by chromatography on Sepharose CL-4B-2.5 to 1.5M ammonium sulfate in 10mM hydrochloric acid at 4 degrees. Both unit-disaccharide composition and molecular-size distribution clearly affected the fractionation. Comparison of this fractionation with the fractionation on Sepharose 6B gel in 0.2M sodium chloride revealed that the former is distinctly superior to the latter. The fractionation on Sepharose CL-4B in the presence of ammonium sulfate also showed that the chondroitin sulfate C molecules having a larger molecular size contain generally more chondroitin 6-sulfate units (as major constituent) and less chondroitin disulfate units (D type, as minor constituent) than those having a smaller molecular size).
D000645:Ammonium Sulfate; D000818:Animals; D002356:Cartilage / Q000032:analysis*; D002807:Chondroitin / Q000031:analogs & derivatives*; D002809:Chondroitin Sulfates / Q000302:isolation & purification*; D002850:Chromatography, Gel / Q000379:methods; D012685:Sepharose / Q000031:analogs & derivatives; D012754:Sharks
C035771:Sepharose CL 4B; D002807:Chondroitin; D002809:Chondroitin Sulfates; D012685:Sepharose; D000645:Ammonium Sulfate