Phosphatidic acid-induced calcium mobilization in osteoblasts.
Phosphatidic acid (PA) evoked a transient increase in the cytosolic free Ca2+ concentration ([Ca2+]i) in osteoblasts isolated from neonatal mouse calvaria. This increase was observed in both low (below 150 microM) and high (1.26 mM) Ca2+-containing medium. In contrast, other phospholipids, such as phosphatidylethanolamine, phosphatidylcholine, and phosphatidylinositol, failed to increase [Ca2+]i in osteoblasts. In high Ca2+-containing medium, A23187 also increased [Ca2+]i in the cells, but the mode of the change was different from that in the case of PA. These results suggest that PA may induce Ca2+-mediated cellular responses through Ca2+ release from intracellular stores in osteoblasts.
D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D010006:Osteoblasts / Q000187:drug effects* / Q000378:metabolism; D010712:Phosphatidic Acids / Q000494:pharmacology*; D010743:Phospholipids / Q000494:pharmacology
D010712:Phosphatidic Acids; D010743:Phospholipids; D000001:Calcimycin; D002118:Calcium