Rapid method for determining the beta-lactamase-inducing potency of drugs.
A rapid semiquantitative method for determining the beta-lactamase inducing potency of drugs was developed. Bacteria carrying a gene for inducible beta-lactamase expression were inoculated at a concentration of 10(8) CFU/ml into microtiter plates for determination of MICs, which were recorded after 4 h of incubation. A suitable chromogenic beta-lactamase substrate was then added, and after incubation for another 3 h colour changes were monitored.
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D004352:Drug Resistance, Microbial; D004755:Enterobacteriaceae / Q000187:drug effects / Q000201:enzymology / Q000235:genetics; D004790:Enzyme Induction / Q000187:drug effects; D006801:Humans; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000201:enzymology / Q000235:genetics; D013211:Staphylococcus aureus / Q000187:drug effects / Q000201:enzymology / Q000235:genetics; D001618:beta-Lactamases / Q000096:biosynthesis* / Q000235:genetics; D047090:beta-Lactams
D000900:Anti-Bacterial Agents; D047090:beta-Lactams; D001618:beta-Lactamases