title: Mutagenicity of lead chromate in Drosophila melanogaster in the presence of nitrilotriacetic acid (NTA).
abstract: By using the sex-linked recessive lethal mutation test in Drosophila melanogaster (standard Basc scheme) we analysed the mutagenic effects of treatments by feeding with nitrilotriacetic acid (NTA: 5 X 10(-2) M), with the insoluble Cr(VI) compound lead chromate, PbCrO4 (supernatant of 4.6 X 10(-4)-M suspension in which the actual concentration was 0.06 gamma/ml as Cr(VI)) and with both compounds preincubated at 3 relative ratios (NTA: 5 X 10(-2) M; PbCrO4: 4.6 X 10(-4), 4.6 X 10(-5) and 9.2 X 10(-6) M, respectively). The estimation of mutation frequencies was done at different developmental stages of the germ cells, namely spermatozoa, spermatids and spermatocytes. Ethyl methanesulphonate (EMS: 5 X 10(-3) M) was used as the reference positive control, with clearly mutagenic results. Treatments with NTA or with PbCrO4 alone did not induce any significant increase of the mutation frequency. PbCrO4 at the 3 concentrations tested was completely soluble in the 5 X 10(-2)-M NTA solution, and the mixture of NTA and PbCrO4 induced significant increases of the frequency of sex-linked lethal mutations, with a significant dose-effect relationship with respect to PbCrO4, apparently as a result of the interaction of the compounds and subsequent release of the genotoxic heavy-metal Cr(VI) ions. This result indicates an important synergistic action of NTA with PbCrO4 under the conditions described.
mesh_terms: D000085:Acetates / Q000494:pharmacology*; D000818:Animals; D002840:Chromates / Q000494:pharmacology*; D004331:Drosophila melanogaster / Q000187:drug effects*; D004357:Drug Synergism; D005804:Genes, Lethal / Q000187:drug effects*; D005808:Genes, Recessive / Q000187:drug effects*; D007854:Lead / Q000494:pharmacology*; D008297:Male; D009571:Nitrilotriacetic Acid / Q000494:pharmacology*; D013091:Spermatogenesis / Q000187:drug effects
chemical_list: D000085:Acetates; D002840:Chromates; D007854:Lead; C031895:lead chromate; D009571:Nitrilotriacetic Acid