Prevention of intra-abdominal abscesses by fibrinolysis using recombinant tissue plasminogen activator.
We studied whether intraperitoneal fibrinolysis using recombinant tissue plasminogen activator (t-PA) could prevent abscess formation in the rat model of intra-abdominal infection caused by fibrin clots. Single-dose administration of t-PA at concentrations greater than 0.005 mg/mL at surgery or delivered by open lavage within 1 h of surgery completely obviated abscess formation induced by Bacteroides fragilis-infected clots without altering mortality (approximately 0.6%). With mixed Escherichia coli-B. fragilis clots, t-PA increased the mortality rate from the control level of 43.8% to 81.3% but prevented abscesses in survivors. This increased mortality rate was probably due to an acute E. coli bacteremia. Antibiotics plus t-PA prevented both mortality and abscess formation. Intraperitoneal fibrinolysis may be a useful adjunct to antibiotics and appropriate surgery in the management of intra-abdominal infection.
D000038:Abscess / Q000517:prevention & control*; D000818:Animals; D001442:Bacteroides Infections / Q000150:complications / Q000517:prevention & control*; D001441:Bacteroides fragilis; D004195:Disease Models, Animal; D004927:Escherichia coli Infections / Q000150:complications / Q000517:prevention & control*; D005342:Fibrinolysis; D008297:Male; D010538:Peritonitis / Q000150:complications / Q000517:prevention & control*; D051381:Rats; D011919:Rats, Inbred Strains; D011994:Recombinant Proteins / Q000627:therapeutic use; D010959:Tissue Plasminogen Activator / Q000037:antagonists & inhibitors / Q000627:therapeutic use*
D011994:Recombinant Proteins; D010959:Tissue Plasminogen Activator