Identification of a DNA binding protein that recognizes the nonamer recombinational signal sequence of immunoglobulin genes.
Extracts of nuclei from B- and T-lymphoid cells contain a protein that binds specifically to the conserved nonamer DNA sequence within the recombinational signals of immunoglobulin genes. Complexes with DNA fragments from four kappa light-chain joining (J) segments have the same electrophoretic mobility. Nonamer-containing DNA fragments from heavy-chain and light-chain genes compete for binding. Within the 5'-flanking DNA of the J kappa 4 gene segment, the binding site has been localized to a 27-base-pair interval spanning the nonamer region. The binding activity is recovered as a single peak after ion-exchange chromatography. The site of binding of the protein and its presence in nuclei of lymphoid cells suggest that it may function in the assembly of immunoglobulin genes.
D000818:Animals; D001665:Binding Sites; D002417:Cattle; D002852:Chromatography, Ion Exchange; D004268:DNA-Binding Proteins / Q000302:isolation & purification* / Q000378:metabolism; D005803:Genes, Immunoglobulin; D007144:Immunoglobulin J-Chains / Q000235:genetics*; D007134:Immunoglobulin Switch Region; D011485:Protein Binding; D011995:Recombination, Genetic
D004268:DNA-Binding Proteins; D007144:Immunoglobulin J-Chains