The presence of chondroitin sulfate in parotid secretory granules and saliva of the rat.
The presence of chondroitin sulfate in secretory granules of the rat parotid gland and its saliva was revealed by radioactive sulfate incorporation, followed by isolation and partial characterization of the sulfated species contained within the granules and in the parotid saliva. 35SO4 was incorporated into chromatographically identical macromolecular material both in vitro, in a gland-slice system followed by isolation of granule contents, and in vivo as measured in the pure parotid secretion following intravenous administration of 35SO4=. The majority of the 35SO4= label appeared in a peak in the region where the family of acidic proline-rich proteins elute from a DEAE-Sephadex A-50 column. Papain digestion freed the sulfated material from the bulk of protein present in this peak, leaving sulfate-labelled material that chromatographed on Sepharose CL6B as a single peak corresponding to a molecular weight of 13,000 daltons. The ratio of uronic acid to amino sugar in this sulfated peak was 0.56. The sulfated material was susceptible to degradation by chondroitinase AC. The presence of this chondroitin sulfate in secretory granules and saliva is consistent with previous suggestions that sulfated polyanions may play a role in formation and maturation of secretory granules.
D000818:Animals; D002458:Cell Fractionation; D002807:Chondroitin / Q000031:analogs & derivatives*; D002809:Chondroitin Sulfates / Q000032:analysis*; D003594:Cytoplasmic Granules / Q000648:ultrastructure*; D008297:Male; D010306:Parotid Gland / Q000648:ultrastructure*; D051381:Rats; D011919:Rats, Inbred Strains; D012463:Saliva / Q000032:analysis*; D012471:Salivary Proteins and Peptides / Q000302:isolation & purification; D013462:Sulfur Radioisotopes; D014316:Tritium
D012471:Salivary Proteins and Peptides; D013462:Sulfur Radioisotopes; D014316:Tritium; D002807:Chondroitin; D002809:Chondroitin Sulfates