title: Study of the phosphorylatable light chains of skeletal and gizzard myosins by nuclear magnetic resonance spectroscopy.
abstract: 31P and 1H n.m.r. studies of the phosphorylatable light chains from rabbit fast skeletal and chicken gizzard muscles in the isolated state and in the intact myosin molecule indicate that the N-terminal region of the light chain containing the sites of phosphorylation has independent segmental flexibility. The ionization behaviour of serine phosphate in both rabbit skeletal and chicken gizzard P light chains exhibits cooperativity and is compatible with the phosphate group being influenced by neighbouring positively charged side-chains. No marked difference in phosphate ionization behaviour was apparent between the monophosphorylated P light chains of rabbit skeletal and chicken gizzard myosins. From 1H and 31P n.m.r. studies of the overall conformation, side-chain ionization properties and the spectral effects of titration with an anionic paramagnetic reagent bound at the basic N-terminal region, it is concluded that Thr-18 and Ser-19 are phosphorylated in the bisphosphorylated P light chain of gizzard myosin, the latter residue being the site of monophosphorylation. In the presence of F-actin the mobility of the serine phosphate of the P light chain of intact gizzard myosin was reduced. No interaction between the isolated P light chain and F-actin was however detected. These results are discussed with reference to the observed conformational features of the P light chain.
mesh_terms: D000818:Animals; D002645:Chickens; D005895:Gizzard, Avian / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D009130:Muscle, Smooth / Q000378:metabolism*; D009218:Myosins / Q000378:metabolism*; D010766:Phosphorylation; D011487:Protein Conformation; D011817:Rabbits; D012694:Serine / Q000032:analysis; D012965:Sodium Chloride / Q000494:pharmacology; D013912:Threonine / Q000032:analysis
chemical_list: D013912:Threonine; D012965:Sodium Chloride; D012694:Serine; D009218:Myosins