The effect of splenectomy on antibody response to lipopolysaccharide (E. coli) immunization.
The influence of splenectomy on the antibody response to lipopolysaccharide (LPS: E. coli 0128:B12) was investigated in mice. Splenectomy had little effect on the primary response to the LPS. However, the level of IgG anti-LPS antibodies of splenectomized mice was significantly lower than that of sham-operated mice when the mice were immunized 1, 3, and 7 days after the operation and reimmunized 7 days after the first immunization. There was no significant difference in those immunized 30 days after the operation and reimmunized 7 days later. In mice immunized before splenectomy and reimmunized 30 days after splenectomy, the level of IgG anti-LPS antibodies was low, even in the mice splenectomized 30 days after primary immunization. Our results indicate that splenectomy impairs the antibody response to lipopolysaccharides.
D000818:Animals; D000917:Antibody Formation; D001428:Bacterial Vaccines / Q000276:immunology*; D004926:Escherichia coli / Q000276:immunology*; D022361:Escherichia coli Vaccines; D005260:Female; D007114:Immunization; D005779:Immunodiffusion; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D051379:Mice; D008807:Mice, Inbred BALB C; D013156:Splenectomy
D001428:Bacterial Vaccines; D022361:Escherichia coli Vaccines; D007074:Immunoglobulin G; D007075:Immunoglobulin M