Evidence of an anti-A1 inhibited by EDTA.
EDTA is added to some commercial A and B cells as a means of preventing hemolysis in serum testing. Unexpected results in serum testing have been reported with EDTA-dependent agglutinins, but not with agglutinins inhibited by EDTA. A serum sample was found to contain an anti-A1 undetected by serum testing, but resulting in incompatible crossmatches. Studies indicated that reactivity was inhibited by the EDTA present in the diluent of the A reagent red cells.
D000017:ABO Blood-Group System / Q000276:immunology*; D000368:Aged; D001788:Blood Grouping and Crossmatching / Q000379:methods; D004492:Edetic Acid / Q000494:pharmacology*; D005260:Female; D006384:Hemagglutination / Q000187:drug effects*; D006801:Humans; D007202:Indicators and Reagents; D007518:Isoantibodies / Q000032:analysis*
D000017:ABO Blood-Group System; D007202:Indicators and Reagents; D007518:Isoantibodies; D004492:Edetic Acid