[Immunofluorescence location using monoclonal antibodies of prolactin receptors in mammary epithelial cells of lactating rabbits].
Immunofluorescent staining of PRL receptors on frozen sections of lactating rabbit mammary gland with a monoclonal antibody (IgG1 M110) shows that receptors are localized in the cytoplasm of epithelial cells and on short portions of plasma membrane. In vivo treatment by bromocriptine or in vitro treatment of mammary tissue slices by monensin modifies localization of receptors.
D000818:Animals; D000911:Antibodies, Monoclonal; D001971:Bromocriptine / Q000494:pharmacology; D002462:Cell Membrane / Q000032:analysis; D003593:Cytoplasm / Q000032:analysis; D004848:Epithelium / Q000032:analysis; D005260:Female; D005455:Fluorescent Antibody Technique; D006651:Histocytochemistry; D007774:Lactation / Q000378:metabolism*; D008321:Mammary Glands, Animal / Q000032:analysis* / Q000187:drug effects / Q000378:metabolism; D008854:Microscopy, Electron; D008985:Monensin / Q000494:pharmacology; D011247:Pregnancy; D011817:Rabbits; D011981:Receptors, Prolactin / Q000032:analysis* / Q000378:metabolism
D000911:Antibodies, Monoclonal; D011981:Receptors, Prolactin; D001971:Bromocriptine; D008985:Monensin
Localisation par immunofluorescence des récepteurs de la prolactine dans les cellules épithéliales mammaires de lapines en lactation à l'aide d'un anticorps monoclonal.