GC-7 cells are growth arrested by cytochalasin D at two different points in the cell cycle.
GC-7 cells, a cell line from African green monkey kidney, which had been growth arrested in G0 phase by serum deprivation, entered S phase 15 h after serum stimulation. They were blocked from entering S phase in the presence of 0.6 micrograms/ml of cytochalasin D. The cells growth arrested between G0 and S phase by cytochalasin D entered S phase 6 h following the removal of the drug. The progression of S, G2, and M phases was not affected by cytochalasin D. On the other hand, when G0-arrested GC-7 cells were stimulated with serum for 23 h up to a late S/G2 phase and then cultured in the presence of cytochalasin D, or when an exponentially growing culture was treated with the drug, the cells were growth arrested at a point 15 h, not 6 h, before the next S phase. This point of growth arrest is kinetically similar to G0 phase, both occur 15 h before S phase, but is different from G0 in terms of c-fos expression after release from the block.
D000818:Animals; D002453:Cell Cycle / Q000187:drug effects*; D002460:Cell Line; D002522:Chlorocebus aethiops; D015638:Cytochalasin D; D003572:Cytochalasins / Q000494:pharmacology*; D005786:Gene Expression Regulation; D007399:Interphase / Q000187:drug effects; D011518:Proto-Oncogene Proteins / Q000235:genetics; D016760:Proto-Oncogene Proteins c-fos; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger / Q000235:genetics; D013997:Time Factors
D003572:Cytochalasins; D011518:Proto-Oncogene Proteins; D016760:Proto-Oncogene Proteins c-fos; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger; D015638:Cytochalasin D