IFN-gamma inhibits c-myc gene expression by impairing the splicing process in a colony-stimulating factor dependent murine myeloid cell line.
Proliferation of NSF-60.8 cells, a CSF-dependent murine myeloid cell line, is strongly inhibited by murine IFN-gamma. Northern analysis of growth arrested, NSF-60.8 cells activated by addition of CSF in the presence or absence of IFN-gamma indicated that IFN-gamma inhibited the induction of c-myc mRNA steady state level by CSF. The effect was observed as early as 30 min after induction and the inhibition was complete after 20 h. IFN-gamma did not impair the transcriptional activation of c-myc gene, and it had only a slight destabilizing effect on the mature c-myc message. Study of the processing steps of c-myc mRNA precursor indicated that in the presence of IFN-gamma, a putative 3.6-kb splice intermediate accumulated instead of the mature message, suggesting that IFN-gamma inhibits the splicing of c-myc precursor.
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D003115:Colony-Stimulating Factors / Q000494:pharmacology*; D006131:Growth Inhibitors / Q000494:pharmacology; D007371:Interferon-gamma / Q000494:pharmacology*; D007951:Leukemia, Myeloid / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D051379:Mice; D011519:Proto-Oncogenes / Q000187:drug effects*; D012322:RNA Precursors / Q000096:biosynthesis; D012326:RNA Splicing / Q000187:drug effects*; D012333:RNA, Messenger / Q000037:antagonists & inhibitors / Q000302:isolation & purification; D014158:Transcription, Genetic / Q000187:drug effects
D003115:Colony-Stimulating Factors; D006131:Growth Inhibitors; D012322:RNA Precursors; D012333:RNA, Messenger; D007371:Interferon-gamma