title: The effect of adherence on the in vitro induction of cytocidal activity by macrophages.
abstract: The effects of macrophage adherence to plastic on tumoricidal activity were investigated. In order to do so, an agarose culture system was developed to provide a means for maintaining non-adherent macrophages in vitro. We were not able to show any effects of adherence on tumour cell lysis by activated macrophages. On the other hand, in vitro activation of non-adherent macrophages was possible only if adherence was replaced by another triggering signal. This requirement was more significant in the A/J mouse strain, where non-adherent macrophages required longer activation periods. Hence we propose that adherence might provide a second signal for the in vitro induction of tumour killing. The biological significance of adherence that is relevant to tumour killing is discussed.
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002478:Cells, Cultured; D003602:Cytotoxicity, Immunologic; D005260:Female; D008262:Macrophage Activation; D008264:Macrophages / Q000276:immunology / Q000502:physiology*; D008297:Male; D012515:Mast-Cell Sarcoma / Q000276:immunology; D051379:Mice; D008805:Mice, Inbred A; D008809:Mice, Inbred C3H; D012685:Sepharose; D014407:Tumor Cells, Cultured / Q000276:immunology
chemical_list: D012685:Sepharose