A one-step Du test in microplates.
A one-step Du test, developed for use in automated microplate systems, uses anti-D with 0.6 percent dextran to potentiate the reaction. Because the washing and reagent-adding steps of the antiglobulin test are not required, the Du test can be performed in the same microplate as the ABO/Rh test. A set of reactions prepared with this technique was visually interpreted and also classified by an automated microplate ABO/Rh system. Visual interpretation of reactions resulted in a sensitivity and specificity close to those of the antiglobulin test, although the sensitivity of the test was reagent-dependent. When the automated microplate blood grouping system was used, the test was not as sensitive or as specific as the antiglobulin test, although it may be sufficient for many applications.
D001322:Autoanalysis / Q000191:economics / Q000379:methods; D001788:Blood Grouping and Crossmatching / Q000191:economics / Q000379:methods*; D006801:Humans; D007202:Indicators and Reagents; D012204:Rh-Hr Blood-Group System
D007202:Indicators and Reagents; D012204:Rh-Hr Blood-Group System