title: Double diffusion and ELISA for the detection of IgG and IgM antibodies against Micropolyspora faeni.
abstract: Fifty-eight sera of subjects with suspected farmer's lung disease (FLD) were tested by double immunodiffusion and ELISA for the detection of IgG and IgM antibodies against Micropolyspora faeni. By comparing the two methods, we obtained a high concordance of results for IgG, but not so high for IgM. ELISA is therefore a sensitive and specific test in determining antibody concentration.
mesh_terms: D000192:Actinomycetales / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005203:Farmer's Lung / Q000175:diagnosis / Q000276:immunology; D006801:Humans; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D007074:Immunoglobulin G / Q000032:analysis*; D007075:Immunoglobulin M / Q000032:analysis*; D007525:Isoelectric Focusing
chemical_list: D007074:Immunoglobulin G; D007075:Immunoglobulin M