Selective secretion of only the urokinase plasminogen activator from rat islets of Langerhans.
Rat islets of Langerhans produce plasminogen activators (PA). The islet level of PA and the enzyme secreted in the islet incubation medium, are regulated by agents which modulate insulin synthesis and release. The cellular PA activity is due to 2 molecular forms of the enzyme, a Mr 72,000 and a Mr 48,000 species found in almost equal proportions. But the enzyme activity in the medium is due to only the lower molecular weight form. It is shown that this enzyme activity in the medium is due to immunologically identifiable urokinase type PA (uPA) and the cellular enzyme activity is due to both, uPA and tissue-type PA (tPA).
D000818:Animals; D046508:Culture Techniques; D007515:Islets of Langerhans / Q000201:enzymology*; D008970:Molecular Weight; D051381:Rats; D011919:Rats, Inbred Strains; D010959:Tissue Plasminogen Activator / Q000378:metabolism; D014568:Urokinase-Type Plasminogen Activator / Q000378:metabolism*
D010959:Tissue Plasminogen Activator; D014568:Urokinase-Type Plasminogen Activator