title: The TX;Y test for the detection of nondisjunction and chromosome breakage in Drosophila melanogaster. II. Results of female exposures.
abstract: The TX; Y test is a short-term assay for the detection of sex-chromosome nondisjunction and chromosome breakage in Drosophila melanogaster. It has been used in previous work following the exposure of males. In this work, females are exposed. When females are the exposed parent, only chromosome gain can be detected. Positive results for the induction of aneuploidy were obtained following exposures of females to X-rays, 10 degrees C cold shock, and colchicine. No increase in aneuploidy was obtained following exposures of females to DMSO and trifluralin. Comparison with similar work in males reveals no consistent pattern concerning the more appropriate sex to use for aneuploidy testing in Drosophila, as colchicine was found to be positive in females only and DMSO and trifluralin were effective in males only. Further work is necessary to validate the TX; Y test and to understand the relative efficacy of female and male exposures to aneuploidy inducing agents in Drosophila.
mesh_terms: D000782:Aneuploidy; D000818:Animals; D003078:Colchicine / Q000633:toxicity; D003080:Cold Temperature; D004121:Dimethyl Sulfoxide / Q000633:toxicity; D004307:Dose-Response Relationship, Radiation; D004331:Drosophila melanogaster; D005260:Female; D005821:Genetic Techniques; D009630:Nondisjunction, Genetic; D009865:Oocytes / Q000187:drug effects / Q000528:radiation effects; D012727:Sex Characteristics; D012730:Sex Chromosomes / Q000187:drug effects / Q000528:radiation effects; D014274:Trifluralin / Q000633:toxicity
chemical_list: D014274:Trifluralin; D003078:Colchicine; D004121:Dimethyl Sulfoxide