[Immunochemical identification and study of interspecies thymus antigen-2].
A new interspecific animal thymus antigen (AgT-2) was identified. It was shown that AgT-2 is a microglobulin with a molecular mass about 12 kDa, electrophoretic mobility of alpha 1-globulins and isoelectric point of 4.6. Heating of the protein to 80 degrees C for 30 min did not lead to the loss of its immunochemical activity. AgT-2 was identified in extracts of bovine fetal thymus, spleen and liver. It was discovered in the extracts of the lung and colon of adult animals. Cross-reactions were found between bovine, walrus, deer and chicken AgT-2. This fact confirms that AgT-2 is interspecies-specific. AgT-2 was not identified in men, dogs, mice, rats and rabbits. Immunochemical research of immunocorrecting preparations revealed that AgT-2 is a constant component of T-activin, thymalin, thymosin and TP1-Serono and its concentration varies from 1.6 to 3.2%. It has been stated that immunochemical test for AgT-2 can be used as a marker in the production and standardization of active thymus factors.
D000818:Animals; D000941:Antigens / Q000032:analysis*; D002417:Cattle; D002645:Chickens; D003670:Deer; D006801:Humans; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D007525:Isoelectric Focusing; D010455:Peptides / Q000032:analysis; D012756:Sheep; D013045:Species Specificity; D013552:Swine; D013947:Thymosin / Q000032:analysis; D013949:Thymus Extracts / Q000032:analysis; D013950:Thymus Gland / Q000276:immunology*; D013951:Thymus Hormones / Q000032:analysis*; D014856:Walruses
D000941:Antigens; D010455:Peptides; D013949:Thymus Extracts; D013951:Thymus Hormones; D013947:Thymosin; C032805:thymalin; C036191:T-activin
Immunokhimicheskaia identifikatsiia i izuchenie mezhvidovogo antigena-2 timusa.