title: Transmission electron microscopy of the morphological relationship between fibroblasts and pulp calcifications in temporary teeth.
abstract: Calcifications found in the coronal pulps of primary teeth extracted in an 8-year-old child were studied by TEM. Different types of relationship were observed between fibroblasts and pulp calcifications: extension of cell processes towards calcifications, modelling of the cells upon calcifications, internalizing process of calcifications. Fibroblasts proved to be able to enclose small pulp calcifications within intracytoplasmic vesicles. There was no evidence of any active role played by fibroblasts in the genesis of pulp calcifications. It was shown that collagen fibres could be involved or not in the mineralizing process. It is suggested that mitochondria might provide an adequate environment for initial mineralization. It is likely that the role played by the cytoskeleton in the internalizing process of calcifications is important.
mesh_terms: D002648:Child; D003094:Collagen; D003593:Cytoplasm / Q000648:ultrastructure; D003782:Dental Pulp / Q000166:cytology*; D003784:Dental Pulp Calcification / Q000473:pathology*; D005347:Fibroblasts / Q000648:ultrastructure*; D006801:Humans; D007425:Intracellular Membranes / Q000648:ultrastructure; D008854:Microscopy, Electron; D014094:Tooth, Deciduous / Q000648:ultrastructure*
chemical_list: D003094:Collagen