Cell-type specific processing of neuroendocrine precursor proteins using vaccinia recombinants.
The cell-type specific processing of human pro-enkephalin was determined using a vaccinia recombinant (VV:hPE). The results show that all cell types infected with VV:hPE efficiently synthesize pro-enkephalin following cleavage of the signal peptide after Ala24. In addition, pro-enkephalin is shown to undergo N-linked glycosylation as well as other post-translational modifications. However, only one cell line. AtT-20, was able to efficiently cleave pro-enkephalin to smaller peptides including Met-enkephalin. Some results have been previously reported (Science 232, 1641-1643).
D000595:Amino Acid Sequence; D001483:Base Sequence; D002472:Cell Transformation, Viral; D004745:Enkephalins / Q000235:genetics*; D006728:Hormones / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D011498:Protein Precursors / Q000235:genetics*; D011499:Protein Processing, Post-Translational; D011994:Recombinant Proteins / Q000302:isolation & purification; D014616:Vaccinia virus / Q000235:genetics*
D004745:Enkephalins; D006728:Hormones; D011498:Protein Precursors; D011994:Recombinant Proteins; C029992:proenkephalin