Granulopoietic effects of colony-stimulating factor obtained from urine of patients with aplastic anemia on normal and cyclophosphamide-treated mice.
Colony-stimulating factor (CSF) was partially purified from urine of patients with aplastic anemia using DEAE-cellulose and concanavalin A-Sepharose. This partially purified CSF caused significant neutrophilia in the peripheral blood of normal mice by (a) single or continual intraperitoneal injection(s) in vivo, and also revealed a specific activity of 1.4 x 10(3) U/absorbance unit (AU) at 280 nm in vitro, with less than 1 ng/AU endotoxin. In addition, this CSF induced faster recoveries of neutrophils in the peripheral blood and progenitor spleen cells of cyclophosphamide (CY)-treated mice. These findings suggest that the CSF used in this study accelerated the differentiation of the granulocytic cells and the proliferation of granulocyte colony-forming units in the spleen. These effects contributed to a rapid recovery from neutropenia in mice treated with CY.
D000741:Anemia, Aplastic / Q000652:urine*; D000818:Animals; D001853:Bone Marrow / Q000473:pathology; D003115:Colony-Stimulating Factors / Q000502:physiology / Q000652:urine*; D003520:Cyclophosphamide / Q000494:pharmacology*; D006098:Granulocytes / Q000502:physiology*; D006410:Hematopoiesis / Q000187:drug effects*; D006412:Hematopoietic Stem Cells; D006801:Humans; D007958:Leukocyte Count / Q000187:drug effects; D008297:Male; D051379:Mice; D009000:Monocytes / Q000502:physiology; D009504:Neutrophils; D013154:Spleen / Q000473:pathology
D003115:Colony-Stimulating Factors; D003520:Cyclophosphamide