Chemotactic response of human neutrophils to N-acetyl chitohexaose in vitro.
N-Acetyl chitohexaose (NACOS-6) was able to display chemotactic response of human neutrophils in vitro. In order to analyze the mechanism, a series of chemotaxis studies by means of neutrophils treated with inhibitors of phospholipase A2, cyclooxygenase, or lipoxygenase to NACOS-6 was conducted. The treatment of neutrophils with inhibitors of phospholipase A2 or cyclooxygenase resulted in decrease of number of migrated cells. However, the lipoxygenase inhibitors did not exhibit the same effect. On the other hand, the treatment of neutrophils with inhibitors of phospholipase A2 or lipoxygenase resulted in decrease of chemotactic response to Formyl-Met-Leu-Phe (FMLP), although the cyclooxygenase inhibitors did not inhibit chemotaxis of neutrophils. Neutrophils added to exogenous prostaglandin E2 (PGE2) caused an enhanced chemotactic response to NACOS-6. These results indicate that the mechanism of chemotactic response to NACOS-6 was different from that of FMLP, and that the response was enhanced by PGE2 released from the neutrophils with stimulation of NACOS-6.
D000328:Adult; D001241:Aspirin / Q000494:pharmacology; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D016861:Cyclooxygenase Inhibitors; D015237:Dinoprost; D015232:Dinoprostone; D004357:Drug Synergism; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology; D016859:Lipoxygenase Inhibitors; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology*; D009504:Neutrophils / Q000187:drug effects*; D009844:Oligosaccharides / Q000494:pharmacology*; D010741:Phospholipases A / Q000037:antagonists & inhibitors; D054467:Phospholipases A2; D011458:Prostaglandins E / Q000494:pharmacology; D011460:Prostaglandins F / Q000494:pharmacology
D016861:Cyclooxygenase Inhibitors; D016859:Lipoxygenase Inhibitors; D009844:Oligosaccharides; D011458:Prostaglandins E; D011460:Prostaglandins F; C056529:N-acetylchitohexaose; D009240:N-Formylmethionine Leucyl-Phenylalanine; D015237:Dinoprost; D010741:Phospholipases A; D054467:Phospholipases A2; D015232:Dinoprostone; D001241:Aspirin; D007213:Indomethacin