Characterization of antisera raised against hypocalcin (teleocalcin) purified from corpuscles of Stannius of rainbow trout, Salmo gairdneri.
Highly specific antisera were raised against hypocalcin, a 54-kDa glycoprotein purified from the corpuscles of Stannius (CS) of rainbow trout, Salmo gairdneri. The specificity of the antisera was determined by using Ouchterlony's double immunodiffusion test, radioimmunoassay (RIA), enzyme-linked immunosorbent assay (ELISA), and immunocytochemistry. In the double immunodiffusion test, a single precipitin line was formed between the antisera and hypocalcin. Both RIA and ELISA studies showed that serial dilutions of hypocalcin, teleocalcin, and CS extracts produced dose-response curves, whereas rat FSH, chicken LH, bovine TSH, salmon calcitonin, bovine parathyroid hormone, and human angiotensins I and II failed to cross-react with the antiserum. Immunoreactive hypocalcin was demonstrated in the plasma of a teleost (flounder), but not of an elasmobranch (dogfish). In the immunocytochemistry, most of the gland cells showed strong immunoreaction with the antisera, whereas some cells displayed no immunoreactivity.
D000818:Animals; D004702:Endocrine Glands / Q000032:analysis* / Q000166:cytology; D004797:Enzyme-Linked Immunosorbent Assay; D006023:Glycoproteins / Q000032:analysis* / Q000276:immunology; D006054:Goldfish; D006728:Hormones; D007106:Immune Sera; D005779:Immunodiffusion; D007124:Immunoenzyme Techniques; D011863:Radioimmunoassay; D014337:Trout
D006023:Glycoproteins; D006728:Hormones; D007106:Immune Sera; C051795:teleocalcin