title: The complete amino acid sequence of a pea (Pisum sativum) seed lipoxygenase predicted from a near full-length cDNA.
abstract: A near full-length cDNA for a pea (Pisum sativum) seed lipoxygenase was isolated and sequenced. It has a protein coding sequence (2583 bp), 5' (59 bp) and 3' (191 bp) non-coding regions, and a poly(A) tail (20 bp). The predicted amino acid sequence indicates a polypeptide of Mr 97,628 that shows about 86% amino acid identity with a soya-bean lipoxygenase 3 protein sequence [Yenofsky, Fine & Liu (1988) Mol. Gen. Genet. 211, 215-222]. The cDNA directs the transcription of mRNA that can be translated to give an anti-lipoxygenase-precipitable polypeptide in vitro.
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D004247:DNA / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D007887:Fabaceae / Q000201:enzymology*; D008084:Lipoxygenase / Q000096:biosynthesis / Q000235:genetics; D008969:Molecular Sequence Data; D010946:Plants, Medicinal; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics; D012639:Seeds / Q000201:enzymology*; D014158:Transcription, Genetic
chemical_list: D012333:RNA, Messenger; D004247:DNA; D008084:Lipoxygenase