p55 IL-2 receptor mRNA precursors in murine T lymphocyte nuclei.
An unusual family of cDNA clones homologous to human p55 IL-2R sequences was isolated from the murine HT-2 Th cell line. These clones were mapped, partially sequenced, and compared with previously published human and mouse IL-2R sequences. They appear to consist of various combinations of exons and introns, suggesting that they are derived from p55 IL-2R mRNA precursors. The configuration of exons in the splicing intermediates indicates that the murine and human gene organizations are similar and that the 3' end of intron 3 is well conserved between the two species. RNA mapping experiments using nuclear, cytoplasmic, and total RNA and probes derived from various parts of the p55 IL-2R gene support and extend the sequence data. They indicate that detectable amounts of immature p55 IL-2R mRNA are found specifically in the cell nucleus of the HT-2 cell line. Similar data were obtained for the Th cell clone 52.3 and the cytotoxic T cell line CTLL. All these results indicate that the T cell nucleus contains significant amounts of immature p55 IL-2R mRNA.
D000818:Animals; D001483:Base Sequence; D002467:Cell Nucleus / Q000032:analysis; D003001:Cloning, Molecular; D003593:Cytoplasm / Q000032:analysis; D007376:Interleukin-2 / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009710:Nucleotide Mapping; D012322:RNA Precursors / Q000302:isolation & purification*; D012333:RNA, Messenger / Q000302:isolation & purification*; D011971:Receptors, Immunologic / Q000235:genetics*; D015375:Receptors, Interleukin-2; D012260:Ribonucleases; D013601:T-Lymphocytes / Q000032:analysis*
D007376:Interleukin-2; D012322:RNA Precursors; D012333:RNA, Messenger; D011971:Receptors, Immunologic; D015375:Receptors, Interleukin-2; D012260:Ribonucleases