title: [Antibiotic production by variants of Penicillium chrysogenum isolated after protoplast formation and exposure to mutagens at the protoplast stage].
abstract: A procedure for protoplast formation in the penicillin-producing organism Penicillium chrysogenum was developed. The yield of the protoplasts was high, the protoplasts were stable and capable of regeneration. Two types of the protoplast regeneration were revealed. The spores and protoplasts were treated with UV light and N-nitroso-N'-methyl biuret and their effect on production of the antibiotic by the isolated variants was studied. It was shown that the protoplasts of P. chrysogenum were more liable to the mutagenic effect of UV light and nitroso methyl biuret than the fungus conidia. It is possible to use this specific feature in intensification of selection aimed at isolation of highly productive strains of P. chrysogenum.
mesh_terms: D003470:Culture Media; D009154:Mutation; D009602:Nitrosamines / Q000494:pharmacology; D010400:Penicillin G / Q000096:biosynthesis*; D010407:Penicillium / Q000378:metabolism*; D010408:Penicillium chrysogenum / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D011092:Polyethylene Glycols / Q000494:pharmacology; D011523:Protoplasts / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D012965:Sodium Chloride / Q000494:pharmacology; D014466:Ultraviolet Rays
chemical_list: D003470:Culture Media; D009602:Nitrosamines; C008654:N-nitroso-N-methylbiuret; D011092:Polyethylene Glycols; D012965:Sodium Chloride; D010400:Penicillin G
vernacular_title: Antibiotikoobrazovanie u variantov Penicillium chrysogenum, poluchennykh posle protoplastirovaniia i obrabotki mutagenami na stadii protoplastov.