Differences in response of glucuronide and glutathione conjugating enzymes to aflatoxin B1 and N-acetylaminofluorene in underfed rats.
Changes in the hepatic drug/xenobiotic-metabolizing enzymes in underfed rats exposed to aflatoxin B1 and N-acetylaminofluorene were investigated. Neither carcinogen, fed at the level of 10 micrograms and 0.667 mg per 100 g body weight, respectively, over a period of 3 wk, had any significant influence on cytochrome P-450 and aryl hydrocarbon hydroxylase in the undernourished rats. Significantly low activities of UDP-glucuronyltransferase and glutathione S-transferase were observed in food-restricted animals fed on aflatoxin B1. N-acetylaminofluorene, on the other hand stimulated both the enzyme activities in the underfed group, to as much observed in the respective well-fed treated group. UDP-Glucuronyltransferase and glutathione S-transferase in undernutrition seem to respond differently to aflatoxin B1 and N-acetylaminofluorene. Further studies are needed to assess the possible consequences of such alterations.
D015073:2-Acetylaminofluorene / Q000633:toxicity*; D000284:Administration, Oral; D016604:Aflatoxin B1; D000348:Aflatoxins / Q000633:toxicity*; D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases / Q000378:metabolism*; D001835:Body Weight / Q000187:drug effects; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D005508:Food Deprivation / Q000502:physiology*; D005982:Glutathione Transferase / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D009929:Organ Size / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains; D005957:UTP-Glucose-1-Phosphate Uridylyltransferase / Q000378:metabolism
D000348:Aflatoxins; D003577:Cytochrome P-450 Enzyme System; D015073:2-Acetylaminofluorene; D016604:Aflatoxin B1; D001189:Aryl Hydrocarbon Hydroxylases; D005982:Glutathione Transferase; D005957:UTP-Glucose-1-Phosphate Uridylyltransferase