Poly(N-acetyllactosaminyl) oligosaccharides of chromaffin granule membrane glycoproteins.
Poly(N-acetyllactosaminyl) oligosaccharides have been identified, on the basis of their susceptibility to endo-beta-galactosidase, in a large-molecular-size glycopeptide fraction derived from chromaffin granule membrane glycoproteins. The glycoproteins containing poly(N-acetyl-lactosaminyl) oligosaccharides were selectively labeled by treatment of chromaffin granule membranes with endo-beta-galactosidase to expose N-acetylglucosamine residues, followed by incubation with galactosyltransferase and UDP-[14C]galactose. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and fluorography demonstrated specific labeling in the 41-47 kilodalton (kD) region and in a distinct band at 90 kDa. Two-dimensional SDS-PAGE revealed that the poly(N-acetyllactosaminyl) oligosaccharides are predominantly present in glycoprotein IV, together with lesser labeling of glycoproteins II and III, whereas they are absent from dopamine beta-hydroxylase and carboxypeptidase H, which are the major glycoproteins of chromaffin granule membranes.
D000313:Adrenal Medulla / Q000648:ultrastructure; D000818:Animals; D002417:Cattle; D002837:Chromaffin Granules / Q000032:analysis*; D002838:Chromaffin System / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D005690:Galactose / Q000378:metabolism; D005700:Galactosyltransferases / Q000378:metabolism; D006026:Glycoside Hydrolases; D008562:Membrane Glycoproteins / Q000032:analysis* / Q000378:metabolism; D008970:Molecular Weight; D009844:Oligosaccharides / Q000032:analysis*; D011134:Polysaccharides / Q000032:analysis* / Q000378:metabolism; D014531:Uridine Diphosphate Galactose / Q000378:metabolism; D001616:beta-Galactosidase / Q000378:metabolism
D008562:Membrane Glycoproteins; D009844:Oligosaccharides; D011134:Polysaccharides; D014531:Uridine Diphosphate Galactose; C037199:poly-N-acetyllactosamine; D005700:Galactosyltransferases; D006026:Glycoside Hydrolases; C026093:keratan-sulfate endo-1,4-beta-galactosidase; D001616:beta-Galactosidase; D005690:Galactose