Expression of IL-2 receptors on peripheral blood lymphocytes from young adult and aged human donors.
Peripheral blood mononuclear cells from young adult and aged human donors were activated with PHA and the expression of IL-2 receptors was examined. For the detection of IL-2 receptor-positive cells, flow cytofluorometry using monoclonal antibody Clonab IL-2R, fluorescein-conjugated swine anti-mouse IgG and fluorescence activated cell sorter FACS 440 were utilized. The age-related decrease in the expression of IL-2 receptors was observed: the percentage of IL-2 receptor-positive PBMC was substantially higher in the group of young adult donors than in the group of the aged individuals.
D000328:Adult; D000368:Aged; D000375:Aging; D000911:Antibodies, Monoclonal; D006801:Humans; D007376:Interleukin-2 / Q000378:metabolism*; D008213:Lymphocyte Activation; D010835:Phytohemagglutinins / Q000494:pharmacology; D011971:Receptors, Immunologic / Q000378:metabolism*; D015375:Receptors, Interleukin-2
D000911:Antibodies, Monoclonal; D007376:Interleukin-2; D010835:Phytohemagglutinins; D011971:Receptors, Immunologic; D015375:Receptors, Interleukin-2