Separation of anticardiolipin antibodies from lupus anticoagulant on a phospholipid-coated polystyrene column.
We describe two novel developments. The first is the preparation of a "cemented" chromatography column comprising siliconized sand-coated by and cemented together with polystyrene thus providing a large hydrophobic surface area suitable for passive antigen absorption. The second is the coating of such a matrix with phospholipid and its use for separating anticardiolipin antibodies from lupus anticoagulant activity in the plasma of a patient with the lupus obstetric syndrome. This is the first description of the separate identities of these two antibodies.
D000906:Antibodies / Q000302:isolation & purification*; D001779:Blood Coagulation Factors / Q000276:immunology* / Q000302:isolation & purification; D002308:Cardiolipins / Q000276:immunology*; D002845:Chromatography / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D016682:Lupus Coagulation Inhibitor; D010743:Phospholipids; D011137:Polystyrenes
D000906:Antibodies; D001779:Blood Coagulation Factors; D002308:Cardiolipins; D016682:Lupus Coagulation Inhibitor; D010743:Phospholipids; D011137:Polystyrenes