The propagation of avian viruses in a continuous cell line (QT35) of Japanese quail origin.
Seven of nine avian virus families tested (Birnaviridae, Coronaviridae, Herpesviridae, Paramyxoviridae, Poxviridae, Reoviridae, and Retroviridae) were found to replicate in a quail fibroblast cell line, designated QT35, resulting in a cytopathic effect (CPE) visible with the naked eye or by low-power microscopy. In comparison, only one (Paramyxoviridae) of seven mammalian virus families tested produced an observable CPE. Cytopathic changes induced by examined viruses were round cell, syncytial, and focus formation. Trypsin did not promote cytopathic changes by selected CPE-negative avian and mammalian viruses in QT35 cells. Several avian viruses (infectious bursal disease virus, Newcastle disease virus, Canary pox virus, and reovirus) formed plaques under agar. Avian reovirus and infectious bursal disease virus produced similar titers in chicken embryo fibroblast (CEF) and QT35 cell cultures. Chicken-egg-yolk neutralizing-antibody titers to IBDV were comparable in CEF and QT35 cell-culture systems.
D000818:Animals; D001717:Birds / Q000382:microbiology*; D002460:Cell Line; D003370:Coturnix; D003588:Cytopathogenic Effect, Viral; D008322:Mammals / Q000382:microbiology; D009500:Neutralization Tests / Q000662:veterinary; D014776:Virus Cultivation / Q000379:methods*; D014780:Viruses / Q000254:growth & development*