Lectin-like components in the macronuclear replication bands of Euplotes eurystomus.
Upon incubation with fluoresceinylated neoglycoproteins, isolated macronuclei from the ciliated protozoan Euplotes eurystomus display different labelling patterns depending on the nature of the sugar bound to the neoglycoproteins. Specific sugar-binding components (i.e., lectin-like molecules) are associated with presumed nucleoli and with the macronuclear replication bands. This is the first demonstration that DNA synthesis and sugar-binding components are co-localized in an eukaryotic cell.
D000818:Animals; D050260:Carbohydrate Metabolism; D002467:Cell Nucleus / Q000032:analysis* / Q000648:ultrastructure; D002843:Chromatin / Q000032:analysis / Q000648:ultrastructure; D016798:Ciliophora / Q000032:analysis* / Q000648:ultrastructure; D004247:DNA / Q000096:biosynthesis; D004261:DNA Replication; D006023:Glycoproteins; D037102:Lectins / Q000032:analysis*; D008856:Microscopy, Fluorescence; D009940:Organoids / Q000032:analysis* / Q000648:ultrastructure
D002843:Chromatin; D006023:Glycoproteins; D037102:Lectins; D004247:DNA