Accumulation of N-methyl-4-phenylpyridinium ion (MPP+) in human melanoma cell line, HMV-I and -II.
N-Methyl-4-phenylpyridinium ion (MPP+) was found to be accumulated in human melanoma cell lines, HMV-I and -II, which originate from human melanoma and differentiate into subclones, HMV-I and -II. HMV-II cells can produce a large amount of melanin, while the other cells produce less. After 3 day culture of these cells in the presence of 1-100 microM MPP+, MPP+ was accumulated in both types of cells and a much larger amount of MPP+ was accumulated in melanin-rich HMV-II cells than in HMV-I cells, even though the uptake velocity of MPP+ into both types of cells was almost the same. In addition, both types of cells could survive, even with MPP+ accumulation. Protein amounts, a non-specific enzyme, beta-galactosidase activity, and intracellular DOPA concentrations in both types of cells, were not affected by the presence of MPP+. These results suggest that MPP+ is accumulated in non-dopaminergic cells and the accumulation is enhanced by the presence of melanin.
D015655:1-Methyl-4-phenylpyridinium; D002460:Cell Line; D004295:Dihydroxyphenylalanine / Q000378:metabolism; D004298:Dopamine / Q000378:metabolism; D006801:Humans; D008543:Melanins / Q000378:metabolism; D008545:Melanoma / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism; D011726:Pyridinium Compounds / Q000378:metabolism*; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism*; D001616:beta-Galactosidase / Q000378:metabolism
D008543:Melanins; D011506:Proteins; D011726:Pyridinium Compounds; C018362:pheomelanin; C041877:eumelanin; D004295:Dihydroxyphenylalanine; D001616:beta-Galactosidase; D015655:1-Methyl-4-phenylpyridinium; D004298:Dopamine