Enhancement of c-fos expression is associated with activated macrophages.
Unique hybrids (HINS and CANS lines) between macrophages and a myeloma cell line, NS1 initially expressed myeloma functions but later expressed active macrophage functions together with constitutive expression of c-fos gene. Enhancement of c-fos transcription was also observed in activated mouse peritoneal macrophages, and a range of macrophage-stimulating substance was found to induce c-fos transcription kinetic unique to each stimulator including immediate, delayed and prolonged responses in aged HINS-B3 cells, which displayed low levels of steady-state c-fos transcription. It was also found that a significant enhancement of c-fos transcription followed restimulation with either interferon gamma (IFN gamma) or lipopolysaccharide (LPS) after an initial IFN gamma stimulation. Thus it appeared that enhanced c-fos expression was closely connected with macrophage activation. On the other hand, macrophage stimulators suppressed [3H]thymidine incorporation into aged HINS-B3 cells. These results may simply suggest that c-fos expression contributes to macrophase activation but not to cell proliferation. However, it is also possible to speculate that c-fos expression contributes to cell proliferation as a salvage system operating to overcome the suppression during the macrophage activation.
D000818:Animals; D001500:BCG Vaccine / Q000276:immunology; D000001:Calcimycin / Q000494:pharmacology; D002117:Calcitriol / Q000494:pharmacology; D002460:Cell Line; D004247:DNA / Q000096:biosynthesis; D005786:Gene Expression Regulation; D006822:Hybrid Cells; D007700:Kinetics; D008070:Lipopolysaccharides / Q000494:pharmacology; D008222:Lymphokines / Q000494:pharmacology; D008262:Macrophage Activation; D008264:Macrophages / Q000502:physiology*; D051379:Mice; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014158:Transcription, Genetic
D001500:BCG Vaccine; D008070:Lipopolysaccharides; D008222:Lymphokines; D011518:Proto-Oncogene Proteins; D000001:Calcimycin; D009240:N-Formylmethionine Leucyl-Phenylalanine; D004247:DNA; D002117:Calcitriol; D013755:Tetradecanoylphorbol Acetate