A quantitative evaluation of promoter activity by microinjection of chloramphenicol acetyltransferase hybrid genes into tissue culture cells.
The combination of DNA microinjection into tissue culture cells and the chloramphenicol acetyltransferase enzyme assay has been used to determine quantitatively the transcriptional activity of viral or eukaryotic promoters. As shown for a wide range of DNA concentrations and time periods between 2 and 16 h the assay allows the reliable and reproducible determination of promoter activities in defined cellular backgrounds. Only 50 cells are necessary for the test.
D000123:Acetyltransferases / Q000032:analysis / Q000235:genetics*; D000818:Animals; D002478:Cells, Cultured; D015500:Chloramphenicol O-Acetyltransferase; D005347:Fibroblasts / Q000201:enzymology; D000882:Haplorhini; D006822:Hybrid Cells; D007668:Kidney / Q000201:enzymology; D008845:Microinjections; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic
D000123:Acetyltransferases; D015500:Chloramphenicol O-Acetyltransferase