A crosslinked preparation of E. coli beta-D-galactosidase.
beta-D-Galactosidase from E. Coli was crosslinked using glutaraldehyde and two bisimidoesters. With glutaraldehyde and dimethyl adipimidate (DMA), it is possible to obtain preparations having higher activity than the native enzyme. Glutaraldehyde and DMA gave preparations showing enhanced thermal stability. The preparation crosslinked with DMA, when used for continuous hydrolysis of lactose in milk, was found to be significantly better than the native enzyme.
D003432:Cross-Linking Reagents; D004119:Dimethyl Adipimidate; D004120:Dimethyl Suberimidate; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology*; D005696:Galactosidases / Q000378:metabolism*; D005976:Glutaral; D006358:Hot Temperature; D006868:Hydrolysis; D007096:Imidoesters; D007700:Kinetics; D007785:Lactose / Q000378:metabolism; D001616:beta-Galactosidase / Q000378:metabolism*
D003432:Cross-Linking Reagents; D007096:Imidoesters; D004119:Dimethyl Adipimidate; D004120:Dimethyl Suberimidate; C007471:dimethyl dithiobispropionimidate; D005696:Galactosidases; D001616:beta-Galactosidase; D007785:Lactose; D005976:Glutaral