[Molecular cloning and the expression of the genes of amino acid biosynthesis of Corynebacterium glutamicum in Escherichia coli cells].
Cloning of genes for threonine and lysine biosynthesis from Corynebacterium glutamicum was performed in Escherichia coli cells using the plasmid vector lambda pSL5. The cloned genes are identified via complementation of thrB and lysA mutations. The gene complementing thrB of E. coli is located within a 4.1 kb EcoRI fragment of C. glutamicum chromosomal DNA. All the recombinant phasmids complementing the lysA gene of E. coli contain common 2.2 kb and 3.3 kb EcoRI C. glutamicum DNA fragments. The cloned DNA fragments hybridize with identical EcoRI fragments of C. glutamicum chromosomal DNA.
D000596:Amino Acids / Q000096:biosynthesis* / Q000235:genetics; D001435:Bacteriophages / Q000235:genetics; D002876:Chromosomes, Bacterial; D003001:Cloning, Molecular / Q000379:methods*; D003352:Corynebacterium / Q000235:genetics* / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D005822:Genetic Vectors; D008239:Lysine / Q000096:biosynthesis / Q000235:genetics; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D013912:Threonine / Q000096:biosynthesis / Q000235:genetics
D000596:Amino Acids; D004269:DNA, Bacterial; D013912:Threonine; D008239:Lysine
Molekuliarnoe klonirovanie i ékspressiia genov biosinteza aminokislot Corynebacterium glutamicum v kletkakh Escherichia coli.