Complexes of thrombin with secreted platelet proteins.
A labeled 77-kDa complex formed when 125I-thrombin was added to platelet suspensions or to the supernatant solution of ionophore-activated platelets. Prostacyclin inhibited complex formation with whole platelets but not with the supernatant solution of ionophore-activated platelets. This is evidence that the complex formed with a factor secreted from activated platelets. Smaller complexes of 70 and 58 kDa formed between labeled thrombin and lysed platelets. The 77-kDa complex was necessary for the formation of a thrombin-thrombospondin complex.
D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism*; D001798:Blood Proteins / Q000378:metabolism*; D000001:Calcimycin / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D011464:Epoprostenol / Q000494:pharmacology; D006023:Glycoproteins / Q000378:metabolism; D006801:Humans; D013917:Thrombin / Q000378:metabolism*; D019699:Thrombospondins
D001798:Blood Proteins; D006023:Glycoproteins; D019699:Thrombospondins; D000001:Calcimycin; D011464:Epoprostenol; D013917:Thrombin