Import of rat liver mitochondrial transhydrogenase.
The biosynthesis of pyridine dinucleotide transhydrogenase has been studied in isolated rat hepatocytes and in a rabbit reticulocyte-lysate translation system supplemented with either intact isolated rat liver mitochondria or the soluble matrix fraction from isolated mitochondria. In intact hepatocytes, the transhydrogenase precursor was short-lived in the cytosol and was efficiently imported into the membranous fraction. When the cell-free translation mixture was incubated with intact mitochondria, the transhydrogenase precursor was processed to the mature form, to an extent that depended on the amount of added mitochondria. Incubation of the translation mixture with the soluble mitochondria matrix fraction converted the precursor to a mature-sized protein with 75% efficiency, this being blocked by various proteinase inhibitors such as EDTA, 1,10-phenanthroline and leupeptin.
D000818:Animals; D001692:Biological Transport; D004492:Edetic Acid / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D004792:Enzyme Precursors / Q000378:metabolism*; D066298:In Vitro Techniques; D007976:Leupeptins / Q000494:pharmacology; D008099:Liver / Q000201:enzymology; D008930:Mitochondria, Liver / Q000187:drug effects / Q000201:enzymology*; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism*; D009250:NADP Transhydrogenases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D010618:Phenanthrolines / Q000494:pharmacology; D051381:Rats; D012235:Rhodamines / Q000494:pharmacology
D004792:Enzyme Precursors; D007976:Leupeptins; D010618:Phenanthrolines; D012235:Rhodamines; D004492:Edetic Acid; D009247:NADH, NADPH Oxidoreductases; D009250:NADP Transhydrogenases; C032854:leupeptin; C025205:1,10-phenanthroline