Mutation at the APRT locus in Friend erythroleukaemia cells. 2. Sensitivity to mitomycin C induced cytogenetic damage.
Clone 707 of the Friend cell was compared with an APRT-deficient subclone for sensitivity to cell killing and the induction of cytogenetic aberrations by mitomycin C (MMC). Two 16-h doses of MMC were used, 0.1 and 0.15 microgram/ml and cells were scored for aberrations at 16, 33 and 44 h post-treatment. The APRT-deficient subclone showed increased cell killing, a higher frequency of aberrations and a higher frequency of cells with severe cytogenetic damage. It is proposed that APRT may play a role in balancing deoxyribonucleoside triphosphate pools for DNA-repair processes.
D000228:Adenine Phosphoribosyltransferase / Q000235:genetics*; D000818:Animals; D002460:Cell Line; D002869:Chromosome Aberrations; D002999:Clone Cells; D004249:DNA Damage; D004915:Leukemia, Erythroblastic, Acute / Q000201:enzymology; D007942:Leukemia, Experimental / Q000201:enzymology*; D051379:Mice; D016685:Mitomycin; D008937:Mitomycins / Q000633:toxicity*; D009154:Mutation; D010430:Pentosyltransferases / Q000235:genetics*
D008937:Mitomycins; D016685:Mitomycin; D010430:Pentosyltransferases; D000228:Adenine Phosphoribosyltransferase