Prolactin differentially affects bacterial toxin-induced ADP-ribosylation of Nb2 lymphoma cell membrane proteins.
Exposure of lactogen-dependent Nb2 lymphoma cells to prolactin for up to 72 hr caused time- and dose-dependent changes in the ability of specific 38 kDa and 41.5 kDa membrane proteins from these cells to be subsequently ADP-ribosylated by pertussis and cholera toxins, respectively. Whereas the sensitivity of the 41.5 kDa substrate to cholera toxin was already reduced after 1 hour, that of the 38 kDa substrate for cholera toxin was increased for up to 72 hours. These findings suggest that membrane G-proteins may mediate the effects of prolactin binding to its receptor, leading to the proliferation of Nb2 lymphoma cells.
D000246:Adenosine Diphosphate Ribose / Q000378:metabolism*; D001427:Bacterial Toxins / Q000494:pharmacology*; D002772:Cholera Toxin / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D019204:GTP-Binding Proteins / Q000502:physiology; D007700:Kinetics; D008223:Lymphoma / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D008970:Molecular Weight; D009243:NAD / Q000378:metabolism; D037342:Pertussis Toxin; D011388:Prolactin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
D001427:Bacterial Toxins; D008565:Membrane Proteins; D010566:Virulence Factors, Bordetella; D009243:NAD; D000246:Adenosine Diphosphate Ribose; D011388:Prolactin; D002772:Cholera Toxin; D037342:Pertussis Toxin; D019204:GTP-Binding Proteins