RNA-binding properties of hnRNP proteins.
The RNA-binding properties of the hnRNP monoparticle proteins were examined using a renaturing blotting procedure. All 'core' proteins are able to bind single-stranded nucleic acids, probably not sequence-specific. The core proteins C1 and, in one case A2 and B2, are able to bind nucleic acids which are double-stranded or which show a high degree of base-paired regions, among them U1 snRNA, whereas A1, B1 and C2 are unable to bind base-paired nucleic acids. The characteristics of C1 in binding base-paired nucleic acids are especially interesting, since the involvement of C1 in the splicing process has been described.
D001482:Base Composition; D001665:Binding Sites; D002352:Carrier Proteins / Q000032:analysis*; D004247:DNA / Q000032:analysis; D004268:DNA-Binding Proteins / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D006367:HeLa Cells; D000076245:Heterogeneous Nuclear Ribonucleoprotein A1; D034481:Heterogeneous-Nuclear Ribonucleoprotein Group A-B; D034482:Heterogeneous-Nuclear Ribonucleoprotein Group C; D034441:Heterogeneous-Nuclear Ribonucleoproteins; D009696:Nucleic Acids / Q000032:analysis; D012326:RNA Splicing; D012330:RNA, Double-Stranded / Q000032:analysis; D016601:RNA-Binding Proteins; D012261:Ribonucleoproteins / Q000032:analysis*; D014158:Transcription, Genetic
C439788:C1 HNRNP; D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D000076245:Heterogeneous Nuclear Ribonucleoprotein A1; D034481:Heterogeneous-Nuclear Ribonucleoprotein Group A-B; D034482:Heterogeneous-Nuclear Ribonucleoprotein Group C; D034441:Heterogeneous-Nuclear Ribonucleoproteins; D009696:Nucleic Acids; D012330:RNA, Double-Stranded; D016601:RNA-Binding Proteins; D012261:Ribonucleoproteins; C439768:hnRNP A2; D004247:DNA