title: The protein core of the largest proteoglycan monomer of articular cartilage. Gel electrophoretic pattern and tryptophanyl peptide bond cleavage.
abstract: The largest proteoglycan monomer of baboon (Papio papio) articular cartilage was isolated and the protein rich core was obtained after chondroitinase AC II and keratanase digestions. On SDS-PAGE the core yielded a single band with apparent Mr of 290,000. Tryptophanyl peptide bond cleavage of the core with N-chlorosuccinimide/urea gave 4 peptides with apparent Mr of 105,000, 66,000, 62,000 and 56,000
mesh_terms: D000818:Animals; D002236:Carbohydrate Conformation; D002358:Cartilage, Articular / Q000032:analysis*; D002808:Chondroitin Lyases / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D006026:Glycoside Hydrolases; D006820:Hyaluronic Acid / Q000378:metabolism; D008970:Molecular Weight; D010215:Papio; D010455:Peptides / Q000378:metabolism*; D011509:Proteoglycans / Q000032:analysis* / Q000378:metabolism; D013388:Succinimides; D014364:Tryptophan / Q000032:analysis / Q000378:metabolism*; D014508:Urea; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D010455:Peptides; D011509:Proteoglycans; D013388:Succinimides; C015752:N-chlorosuccinimide; D014364:Tryptophan; D014508:Urea; D006820:Hyaluronic Acid; D006026:Glycoside Hydrolases; C026093:keratan-sulfate endo-1,4-beta-galactosidase; D001616:beta-Galactosidase; D002808:Chondroitin Lyases