title: Protein Rou. A human IgA hybrid.
abstract: Protein Rou is a human IgA2 myeloma protein that carries the isoallotype marker n A2m(2). Partial amino acid sequence of its H chain (alpha) shows that the hinge region and the CH2 domain are homologous to alpha 2-chain and the CH1 and the CH3 domains homologous to alpha 1. Moreover, the CH1 domain contains the H-L disulfide bond identical to alpha 1. It is concluded that Rou H chain is a hybrid molecule caused by a recombination between alpha 1 and alpha 2 genes. The recombination event occurred between alpha 1-exon 1 and alpha 2-exon hinge and corresponds to position 222-223 of the alpha-chain.
mesh_terms: D000595:Amino Acid Sequence; D005091:Exons; D052058:Hinge Exons; D006801:Humans; D007127:Immunoglobulin Constant Regions / Q000235:genetics; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D007145:Immunoglobulin kappa-Chains / Q000235:genetics; D009101:Multiple Myeloma / Q000032:analysis; D009194:Myeloma Proteins / Q000235:genetics* / Q000302:isolation & purification; D011995:Recombination, Genetic; D012689:Sequence Homology, Nucleic Acid
chemical_list: D007127:Immunoglobulin Constant Regions; D007143:Immunoglobulin Heavy Chains; D007145:Immunoglobulin kappa-Chains; D009194:Myeloma Proteins; C055049:myeloma protein Rou