Nitrocellulose dot-ELISA for serodiagnosis of schistosomiasis.
The diagnostic value of the nitrocellulose (NC)-ELISA technique originally described for the detection of viral and bacterial antigens was studied for the detection of antibodies in patients with parasitologically confirmed schistosomiasis, using only about 100 ng total protein content per NC disc of Schistosoma mansoni soluble egg antigen supplied by WHO. The results showed excellent sensitivity: 100% (38/38) for S. mansoni cases and 93.6% (44/47) for S. haematobium. The specificity tested on 50 sera of persons from non-endemic areas was also 100%. 34 sera of patients suffering from other parasitoses were also included. 3 sera of 10 from filariasis cases reacted positively by NC-ELISA, but they were also positive by indirect immunofluorescence, indicating a possible undetected Schistosoma infection. One positive reaction among sera from 4 toxocariasis cases was not confirmed by further tests. We conclude that the NC-ELISA can be a useful technique, especially in developing countries where tests with low cost equipment are needed. Large-scale screening studies should be done to evaluate its usefulness under field conditions.
D000818:Animals; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D012548:Schistosoma haematobium; D012552:Schistosomiasis / Q000175:diagnosis*; D012553:Schistosomiasis haematobia / Q000175:diagnosis; D012555:Schistosomiasis mansoni / Q000175:diagnosis; D013045:Species Specificity