Lateral hypothalamic feeding mechanisms: iontophoretic effects of kainic acid, ibotenic acid and 6-hydroxydopamine.
In order to study hunger motivated behavior kainic acid (KA), ibotenic acid (IB) and 6-hydroxydopamine (6-OHDA) were iontophoretically applied to the lateral hypothalamus of rats. Neurotoxins at concentrations between 20-120 mMol were applied with 5-20 microA current for 5-10 min. Tip diameter of glass micropipettes varied between 10-50 micron. Application of KA, IB and 6-OHDA caused temporary body weight loss, hypophagia and hypodipsia. Effects were dose dependent: correlation was found between current strength, tip diameter of pipettes, concentrations of neurotoxins and the extent of cellular damage. Aphagic and adipsic symptoms and the death of animals were only observed after extensive LH lesions. There was no significant difference in food consumption after 24 hr deprivation among groups. Water deprivation and extracellular or intracellular dehydration resulted in a considerable increase in water intake in all animals, however, consumption in lesioned rats was lower than that of controls. These water regulatory disturbances in 6-OHDA treated animals were somewhat less severe. Results show that the bilateral cellular microlesions of the LH and damage of dopaminergic (DA) elements with 6-OHDA both cause disturbances of feeding behavior. Although the severity of symptoms in some respects depends upon the nature of neurotoxic treatment, the basic consequences are essentially similar. It is suggested that the LH "feeding center" and the ascending DA pathways represent a single system involved in the organization of hunger motivated behavior.
D000818:Animals; D001921:Brain / Q000378:metabolism; D002395:Catecholamines / Q000378:metabolism; D004327:Drinking Behavior / Q000187:drug effects / Q000502:physiology; D005106:Exploratory Behavior / Q000187:drug effects / Q000502:physiology; D005247:Feeding Behavior / Q000187:drug effects / Q000502:physiology*; D006892:Hydroxydopamines / Q000494:pharmacology*; D007026:Hypothalamic Area, Lateral / Q000187:drug effects / Q000502:physiology*; D007051:Ibotenic Acid / Q000494:pharmacology*; D007478:Iontophoresis; D007608:Kainic Acid / Q000494:pharmacology*; D008297:Male; D009498:Neurotoxins / Q000494:pharmacology; D010080:Oxazoles / Q000494:pharmacology*; D016627:Oxidopamine; D051381:Rats
D002395:Catecholamines; D006892:Hydroxydopamines; D009498:Neurotoxins; D010080:Oxazoles; D007051:Ibotenic Acid; D016627:Oxidopamine; D007608:Kainic Acid