Binding studies of macrolides, lincosamides and streptogramins to Streptococcus G group using [3H]-erythromycin.
Parameters of [3H]-erythromycin binding to Streptococcus are determined in vivo using both equilibrium and kinetic methods. This binding is saturable, reversible and independent of energetic systems. Whatever the methods used, the binding parameters are identical as 14 nM for the dissociation constant of the complex erythromycin-Streptococcus and a density of binding sites of 11,865 molecules/cell. Other macrolides, streptogramins and lincosamides competitively displaced bound [3H]-erythromycin suggesting that these compounds share common binding sites on the bacteria. In parallel, the MIC values of these antibiotics against Streptococcus are determined by agar dilution method in Mueller-Hinton medium with 5% of horse blood in order to compare the binding and microbiological parameters. A strong correlation (n = 0.863) has been found between the corresponding inhibition constants and MIC values. Such binding studies could be used in conjunction with microbiological assays for primary screening of active analogous or other compounds with interfere with [3H]-erythromycin binding to the bacteria.
D000900:Anti-Bacterial Agents / Q000378:metabolism*; D004917:Erythromycin / Q000378:metabolism*; D007700:Kinetics; D055231:Lincosamides; D018942:Macrolides; D008826:Microbial Sensitivity Tests; D013291:Streptococcus / Q000378:metabolism*; D014316:Tritium; D014769:Virginiamycin / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D055231:Lincosamides; D018942:Macrolides; D014316:Tritium; D014769:Virginiamycin; D004917:Erythromycin