The preparation and properties of novel reversible polymer-protein conjugates. 2-omega-Methoxypolyethylene (5000) glycoxymethylene-3-methylmaleyl conjugates of plasminogen activators.
The preparation of a reagent capable of reversibly attaching polyethylene glycol to proteins and the use of this material in modifying the plasminogen activators urokinase- and tissue-type plasminogen activator are described. The characterisation and the reversible nature of these protein-polymer conjugates are discussed, and some of the in vitro properties of these modified enzymes are explored.
D000818:Animals; D006168:Guinea Pigs; D008299:Maleic Anhydrides; D011092:Polyethylene Glycols; D011108:Polymers; D010959:Tissue Plasminogen Activator / Q000378:metabolism / Q000493:pharmacokinetics; D014568:Urokinase-Type Plasminogen Activator / Q000378:metabolism / Q000493:pharmacokinetics
D008299:Maleic Anhydrides; D011108:Polymers; D011092:Polyethylene Glycols; D010959:Tissue Plasminogen Activator; D014568:Urokinase-Type Plasminogen Activator