Two populations of serotonin-immunoreactive neurons in the frog (Rana esculenta) retina.
Serotonin immunocytochemical staining in retina whole-mounts of Rana esculenta revealed two differently stained amacrine cell populations. Statistical investigations using nearest neighbor analysis and comparison with different theoretical cell distributions suggested that the two serotonin-immunoreactive cell populations are related to functionally different types. The analysis of type A (intensely stained somata, less numerous) revealed a good coincidence with the normal Gaussian distribution indicating a functionally homogeneous population. The distribution of the neurons of type B (weakly stained somata, more numerous) reflects rather a random dot pattern than a regular arrangement. Thus there is evidence that this last type is a heterogeneous cell population consisting of several functionally different types.
D000818:Animals; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes; D009474:Neurons / Q000166:cytology*; D011893:Rana esculenta; D012160:Retina / Q000166:cytology*; D012701:Serotonin / Q000032:analysis* / Q000276:immunology; D013861:Thiocyanates
D005452:Fluoresceins; D005456:Fluorescent Dyes; D013861:Thiocyanates; D012701:Serotonin; D016650:Fluorescein-5-isothiocyanate