[Isolation and various properties of the lytic enzyme preparation from Streptomyces griseus].
Certain optimal parameters were determined for production of a pure enzyme preparation of the GIOx type from S. griseus by precipitating the enzymes from concentrated fermentation broth filtrate. Antibacterial spectrum of the preparation is comparatively narrow. Still, it showed high lytic activity against streptococci and staphylococci. Investigation of the preparation with the electrofocusing technique revealed that the main lysing component was a protein fraction with its isoelectric point within 8-10.5.
D000096:Acetone / Q000494:pharmacology; D001433:Bacteriolysis; D003470:Culture Media; D007526:Isoelectric Point; D010447:Peptide Hydrolases / Q000302:isolation & purification*; D013305:Streptomyces griseus / Q000201:enzymology*
D003470:Culture Media; D000096:Acetone; D010447:Peptide Hydrolases
Poluchenie i nekotorye svoistva liticheskogo fermentnogo preparata iz Streptomyces griseus.