Effect of praziquantel on Schistosoma mansoni eggs: leucine aminopeptidase (LAP) activity and anti-LAP antibodies.
High levels of leucine aminopeptidase (LAP) have been detected in Schistosoma mansoni eggs. In this report we demonstrate that this enzyme is immunogenic in mice infected with either S. mansoni or S. japonicum. The anti-schistosomal agent, praziquantel, causes rapid release of this enzyme from schistosome eggs in vitro. Praziquantel treatment of S. japonicum infected mice is associated with a significant decrease in anti-LAP antibodies. Anti-LAP IgM antibody levels decreased more than did anti-LAP IgG antibodies.
D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis*; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D007106:Immune Sera / Q000276:immunology; D007118:Immunoassay; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D007931:Leucyl Aminopeptidase / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D010063:Ovum / Q000187:drug effects / Q000201:enzymology / Q000276:immunology; D011223:Praziquantel / Q000494:pharmacology*; D012550:Schistosoma mansoni / Q000187:drug effects* / Q000201:enzymology / Q000276:immunology; D012554:Schistosomiasis japonica / Q000276:immunology; D012555:Schistosomiasis mansoni / Q000276:immunology
D000909:Antibodies, Helminth; D007106:Immune Sera; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D011223:Praziquantel; D007931:Leucyl Aminopeptidase