Structural features and antigenic properties of carbohydrate-containing components of Trypanosoma conorhini.
Aqueous and phenolic extracts of Trypanosoma conorhini were fractionated and high molecular weight, carbohydrate-rich fractions obtained. Their antigenic characteristics, reactivity with lectins and partial chemical structure were determined. The major component, the phenolic extract, was electrophoretically diffuse and consisted of 15% protein, 5% phosphorus, hexosamine, and 67% neutral carbohydrate, which contained mannose, galactose, and xylose in a molar ratio of 1.0:1.8:1.8. Chemical analyses and lectin agglutination experiments showed nonreducing end-groups of beta-D-galactopyranose, beta-xylopyranose, and alpha-D-mannopyranose. Phosphate esters occurred, apparently, at O-6 of hexopyranosyl units. Hexosamine was present as nonacetylated units of 2-amino-2-deoxy-alpha-D-glucopyranosyl units that were extremely resistant to acid hydrolysis. On double immunodiffusion tests, the major component gave a precipitation line with rabbit serum against whole cells of Trypanosoma cruzi, suggesting the presence of common antigenic determinant(s) on the cell surface of each trypanosomatid.
D000372:Agglutination Tests; D000818:Animals; D000953:Antigens, Protozoan / Q000032:analysis*; D002241:Carbohydrates / Q000032:analysis / Q000302:isolation & purification; D002850:Chromatography, Gel; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006001:Glycoconjugates / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification; D005779:Immunodiffusion; D014345:Trypanosoma / Q000032:analysis* / Q000276:immunology
D000953:Antigens, Protozoan; D002241:Carbohydrates; D006001:Glycoconjugates