Effect of certain chelating agents on the antibacterial action of silver nitrate.
EDTA and EGTA when used in conjunction with AgNO3 enhanced the antibacterial action of the latter significantly, so that strains of Klebsiella pneumoniae and Staphylococcus aureus resistant to 70 micrograms/ml of AgNO3 were observed to became sensitive to 10 micrograms/ml of this compound. The synergistic effect of EDTA appears to be due to a mechanism other than the removal of lipopolysaccharide from outer membrane, as its effect could be observed in even non-LPS containing gram positive S. aureus cells. Penicillamine, another potent chelator had an opposite effect so that it decreased the toxicity of silver ions.
D000900:Anti-Bacterial Agents / Q000008:administration & dosage*; D002614:Chelating Agents / Q000008:administration & dosage / Q000494:pharmacology*; D004352:Drug Resistance, Microbial; D004357:Drug Synergism; D004492:Edetic Acid / Q000008:administration & dosage / Q000494:pharmacology; D004533:Egtazic Acid / Q000008:administration & dosage / Q000494:pharmacology; D007711:Klebsiella pneumoniae / Q000187:drug effects; D010396:Penicillamine / Q000008:administration & dosage / Q000494:pharmacology; D012835:Silver Nitrate / Q000008:administration & dosage / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000187:drug effects
D000900:Anti-Bacterial Agents; D002614:Chelating Agents; D004533:Egtazic Acid; D012835:Silver Nitrate; D004492:Edetic Acid; D010396:Penicillamine