A rapid and highly predictive in vitro assay for non-steroidal anti-inflammatory agents.
The inhibition of the production of malonyldialdehyde (MDA) in guinea-pig lung homogenates, incubated in the presence of 50 microM arachidonic acid and 1.4 mM adrenaline, has been exploited as a simple and reliable assay to test in vitro non-steroidal anti-inflammatory agents (NSAIA). The inhibitory potencies of a series of reference NSAIA, which correlated fairly well with in vivo anti-inflammatory activity as determined by carrageenin oedema, are herewith reported. The specificity of the assay was also evaluated by testing up to forty miscellaneous drugs: none of these significantly reduced the MDA production.
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000032:analysis* / Q000494:pharmacology; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D005260:Female; D006168:Guinea Pigs; D066298:In Vitro Techniques; D008297:Male; D008315:Malondialdehyde / Q000032:analysis / Q000037:antagonists & inhibitors; D009994:Osmolar Concentration; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism
D000894:Anti-Inflammatory Agents, Non-Steroidal; D008315:Malondialdehyde; D011451:Prostaglandin-Endoperoxide Synthases