Polyamine metabolism as a target for growth inhibition of Acanthamoeba culbertsoni.
Alpha-difluoromethylornithine had no inhibitory effect on the growth of Acanthamoeba culbertsoni, while methylglyoxal bis(guanyl-hydrazone) and sinefungin completely blocked amebic growth at 2.5 mM and 5 micrograms/ml concentrations, respectively. Cyclohexylamine caused only partial inhibition of amebic growth at 50-200 microM concentrations. Methylglyoxal bis(guanyl-hydrazone) inhibited S-adenosylmethionine decarboxylase of A. culbertsoni indicating this enzyme to be the target for its antiamebic action. This enzyme was, however, not inhibited by sinefungin, suggesting an alternative target for growth inhibition by this compound.
D000048:Acanthamoeba / Q000187:drug effects* / Q000254:growth & development; D000241:Adenosine / Q000031:analogs & derivatives* / Q000494:pharmacology; D012437:Adenosylmethionine Decarboxylase / Q000378:metabolism; D000447:Aldehydes / Q000494:pharmacology*; D000818:Animals; D003514:Cyclohexylamines / Q000494:pharmacology*; D000518:Eflornithine / Q000494:pharmacology*; D011073:Polyamines / Q000378:metabolism*; D011765:Pyruvaldehyde / Q000494:pharmacology*
D000447:Aldehydes; D003514:Cyclohexylamines; D011073:Polyamines; D011765:Pyruvaldehyde; D012437:Adenosylmethionine Decarboxylase; D000241:Adenosine; C006235:sinefungin; D000518:Eflornithine