title: Sympathetic regulation of cerebral blood flow during seizures in newborn lambs.
abstract: We examined cerebral blood flow (CBF) regulation by the sympathetic nerves in 12 newborn lambs (3-11 days old) during seizures, a potent reflex stimulator of the sympathetic nervous system. CBF was measured with microspheres, and seizures were induced with bicuculline. In six of these lambs, one hemibrain was denervated (D) chronically by interrupting the ipsilateral cervical sympathetic trunk; the other hemibrain remained innervated (I). Before and after 10, 35, and 70 min of seizures, cerebral gray matter blood flow (mean +/- SE ml.min-1.100 g-1) was, respectively, 12 +/- 3 (9%), 71 +/- 12 (21%), 120 +/- 15 (38%), and 54 +/- 5 (14%) greater (P less than 0.05) in the D than in the I hemibrain. In the cerebral white matter, hippocampus, caudate, and thalamus blood flows to the D and I hemibrains were similar before seizures but during seizures they were 10-39% greater (P less than 0.05) in the D than in the I hemibrain. Midbrain, brainstem, and cerebellum D and I blood flows were always similar. In the other six lambs, acute denervation during seizures increased ipsilateral cerebral gray and hippocampus blood flow by 10-31%, but unilateral electrical stimulation decreased ipsilateral cerebral gray, cerebral white, hippocampus, thalamus, and caudate blood flow by 17-27%. The data demonstrate that, during seizures, sympathetic nerve activity modifies regional CBF and the effect is sustained, suggesting a role for the sympathetic nervous system in newborn CBF regulation.
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001794:Blood Pressure; D002245:Carbon Dioxide / Q000097:blood; D002560:Cerebrovascular Circulation; D009928:Organ Specificity; D010100:Oxygen / Q000097:blood; D010313:Partial Pressure; D012016:Reference Values; D012039:Regional Blood Flow; D012640:Seizures / Q000503:physiopathology*; D012756:Sheep; D013564:Sympathetic Nervous System / Q000502:physiology / Q000503:physiopathology*
chemical_list: D002245:Carbon Dioxide; D010100:Oxygen