The effect of varying illumination on imprinting of Tetrahymena by insulin.
Tetrahymena pyriformis cultures were imprinted with insulin. Hormone binding was reduced in the dark, but alternation of dark and light periods were in this respect more effective than the dark itself. The deviations observed may be attributed, besides the reduced insulin binding by the imprinted cells in the dark, to an enhanced binding by the non-imprinted control cells kept in the dark. It is suggested that the dark-induced structural transformation of the membrane, manifesting among others in a changed hormone binding, may be caused by alterations in haem synthesis due to varying illumination.
D000818:Animals; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D005456:Fluorescent Dyes; D005470:Fluorometry; D007328:Insulin / Q000378:metabolism*; D008027:Light; D008905:Minicomputers; D013769:Tetrahymena pyriformis / Q000378:metabolism* / Q000528:radiation effects; D013861:Thiocyanates
D005452:Fluoresceins; D005456:Fluorescent Dyes; D007328:Insulin; D013861:Thiocyanates; D016650:Fluorescein-5-isothiocyanate