Foreign and endogenous serum protein extravasation during harmaline tremors or kainic acid seizures in the rat: a comparison.
Cerebrovascular permeability to protein (CVP-p) was assessed in rats following the systemic injection of either kainic acid (KA) or harmaline. The extravasation of a foreign (horseradish peroxidase, HRP) or an endogenous (rat immunoglobulin G, IgG) tracer protein was determined using immunohistochemical methods. During KA-induced seizures, an extravasation of both HRP and presumed IgG occurred in similar forebrain loci; a lamina-specific extravasation occurred within the dorsal hippocampus. During harmaline-induced tremors protein extravasation also occurred, but was tracer dependent. HRP reaction product was observed within the inferior olive, the cortex of the cerebellar vermis and the neocortex. However, IgG-like immunoreactivity was only detected within the circumventricular organs of harmaline-treated rats. Because KA, but not harmaline, is neurotoxic, the results are consistent with an influence of endogenous serum protein extravasation on seizure-related hippocampal damage. Possible homeostatic properties of altered CVP-p are also considered.
D000470:Alkaloids / Q000633:toxicity*; D000818:Animals; D001812:Blood-Brain Barrier / Q000187:drug effects*; D005260:Female; D006246:Harmaline / Q000633:toxicity*; D006735:Horseradish Peroxidase / Q000493:pharmacokinetics*; D007143:Immunoglobulin Heavy Chains / Q000493:pharmacokinetics*; D007142:Immunoglobulin gamma-Chains / Q000493:pharmacokinetics*; D007608:Kainic Acid / Q000633:toxicity*; D008032:Limbic System / Q000378:metabolism / Q000503:physiopathology; D010544:Peroxidases / Q000493:pharmacokinetics*; D051381:Rats; D011919:Rats, Inbred Strains; D012640:Seizures / Q000139:chemically induced / Q000378:metabolism* / Q000503:physiopathology
D000470:Alkaloids; D007143:Immunoglobulin Heavy Chains; D007142:Immunoglobulin gamma-Chains; D006246:Harmaline; D006735:Horseradish Peroxidase; D010544:Peroxidases; D007608:Kainic Acid