Mechanism of interferon action. I. Characterization of a 54-kDa protein induced by gamma interferon with properties similar to a cytoskeletal component.
A polypeptide of 54-kDa molecular mass (p54) induced by molecularly cloned human interferon gamma (rIFN-gamma) in human amnion U cells was characterized biochemically and immunologically. Rabbit polyclonal antibody to p54 was prepared using p54 purified from human amnion U cells as the immunogen. As measured by immunoprecipitation of [35S]methionine pulse-labeled extracts or by immunoblot analysis of unlabeled extracts, the synthesis of p54 was greatly elevated in three human cell lines treated with IFN-gamma, amnion U, fibroblast GM2767, and fibroblast F153. Moreover, the rabbit anti-p54 polyclonal antibody preparation cross-reacted with cytoskeleton-associated polypeptides prepared from human cells and from sea urchin embryos. By use of a monoclonal antibody probe directed against the common domain of intermediate filaments (anti-intermediate filament antibody), it was established that intermediate filament components are also induced by rIFN-gamma. Anti-p54 polyclonal antibody cross-reacted with anti-intermediate filament antibody-recognized, rIFN-gamma-induced polypeptides. Thus, it appears that p54 may be an IFN-induced cytoskeleton-associated polypeptide.
D000818:Animals; D000906:Antibodies; D002460:Cell Line; D002478:Cells, Cultured; D003429:Cross Reactions; D003598:Cytoskeletal Proteins / Q000096:biosynthesis*; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D007381:Intermediate Filament Proteins / Q000032:analysis / Q000276:immunology; D008715:Methionine / Q000378:metabolism; D008970:Molecular Weight; D011994:Recombinant Proteins / Q000494:pharmacology; D012995:Solubility
D000906:Antibodies; D003598:Cytoskeletal Proteins; D007381:Intermediate Filament Proteins; D011994:Recombinant Proteins; D007371:Interferon-gamma; D008715:Methionine