Studies on WF-3681, a novel aldose reductase inhibitor. I. Taxonomy, fermentation, isolation and characterization.
WF-3681 was isolated from a cultured filtrate of Chaetomella raphigera as a novel inhibitor of aldose reductase. It was extracted with ethyl acetate and then purified with silica gel chromatography. Its molecular formula was determined to be C13H12O5 by elemental analysis and high resolution electron impact mass spectrometry. IC50 of WF-3681 was 2.5 X 10(-7) M for partially purified aldose reductase of rabbit lens.
D000449:Aldehyde Reductase / Q000037:antagonists & inhibitors*; D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D004791:Enzyme Inhibitors / Q000302:isolation & purification; D005285:Fermentation; D005663:Furans / Q000302:isolation & purification* / Q000494:pharmacology; D003904:Mitosporic Fungi / Q000145:classification* / Q000378:metabolism; D011817:Rabbits; D013401:Sugar Alcohol Dehydrogenases / Q000037:antagonists & inhibitors*
D004791:Enzyme Inhibitors; D005663:Furans; C054351:WF 3681; D013401:Sugar Alcohol Dehydrogenases; D000449:Aldehyde Reductase