Enhanced reaction with Vicia graminea lectin and exposed terminal N-acetyl-D-glucosaminyl residues on a sample of human red cells with Hb M-Hyde Park.
A sample of polyagglutinable red cells was obtained from a healthy individual (group O, N) possessing a hemoglobin (Hb) variant called Hb M-Hyde Park. The sialic acid content of the individual's red cells is 90 percent of normal, and his cells are agglutinated by monoclonal but not lectin anti-Tn, a panel of lectins specific for N-acetylgalactosamine (or galactose), and N-acetylglucosamine. Enhanced agglutination reactions were obtained with Vicia graminea, Ulex europaeus, and human anti-I and -i. Using various enzyme treatments and different methods of labeling cell surface components, two defective cell membrane sites have been identified: one associated with the O-linked oligosaccharides on sialoglycoproteins and the other associated with exposed N-acetylglucosaminyl residues located on membrane components of apparent molecular weights 88,000 to 130,000 and 46,000 to 73,000 (probably the Band 3 and Band 4.5 regions, respectively).
D000117:Acetylglucosamine; D000911:Antibodies, Monoclonal; D004910:Erythrocyte Membrane / Q000032:analysis; D005944:Glucosamine / Q000031:analogs & derivatives; D006026:Glycoside Hydrolases; D006031:Glycosylation; D006386:Hemagglutination Tests; D006449:Hemoglobin M / Q000276:immunology* / Q000302:isolation & purification; D006455:Hemoglobins, Abnormal / Q000276:immunology*; D006801:Humans; D007106:Immune Sera; D037102:Lectins; D008565:Membrane Proteins / Q000097:blood / Q000276:immunology* / Q000302:isolation & purification; D019158:N-Acetylneuraminic Acid; D010206:Papain; D037121:Plant Lectins; D012794:Sialic Acids / Q000032:analysis; D014357:Trypsin; D001616:beta-Galactosidase
D000911:Antibodies, Monoclonal; D006455:Hemoglobins, Abnormal; D007106:Immune Sera; D037102:Lectins; D008565:Membrane Proteins; D037121:Plant Lectins; D012794:Sialic Acids; C042242:Vicia lectins; D006449:Hemoglobin M; D006026:Glycoside Hydrolases; C026093:keratan-sulfate endo-1,4-beta-galactosidase; D001616:beta-Galactosidase; D014357:Trypsin; D010206:Papain; D019158:N-Acetylneuraminic Acid; D005944:Glucosamine; D000117:Acetylglucosamine