Cellular morphometric changes in cat hearts subjected to three hours of regional ischaemia.
The left anterior descending coronary artery (LAD) was occluded for three hours in seven anaesthetized open chest cats. Seven cats served as sham operated controls. Biopsies were collected from the mid-myocardium of the normal, border and ischaemic zones as defined by fluorescein staining and verified by blood flow measurements with radiolabelled microspheres. In the sham operated hearts the biopsies were taken from the mid-myocardium of the lateral wall of the left ventricle. Fractional volumes of mitochondria, myofibrils and remaining cytoplasm as well as data on the outer mitochondrial membrane were obtained by standard point counting techniques. In the LAD occluded hearts we observed a morphologically distinct lateral border zone characterized by a greater swelling of the mitochondria than in the ischaemic zone. However, in this group a more marked oedema of the cytoplasm and a greater percentage of heavily damaged cells were observed in the ischaemic than in the border zone. The ischaemic zone also had the largest water content. Furthermore, cytoplasmic oedema occurred in the normal zone of the LAD occluded cats when compared with the sham operated controls. This indicates that LAD occlusion also affects the normally perfused parts of the heart.
D000818:Animals; D001794:Blood Pressure; D002302:Cardiac Output; D002415:Cats; D003327:Coronary Disease / Q000473:pathology* / Q000503:physiopathology; D003331:Coronary Vessels / Q000502:physiology; D005260:Female; D006321:Heart / Q000502:physiology / Q000503:physiopathology; D006339:Heart Rate; D008297:Male; D008854:Microscopy, Electron; D009206:Myocardium / Q000166:cytology / Q000473:pathology* / Q000648:ultrastructure; D012016:Reference Values