Pitfall in immunocytochemical localization of prostaglandin E2 and prostaglandin F2 alpha in ovaries of adult rats.
By means of specific antisera against PGE2 and PGF2 alpha, sections of rat ovaries were investigated immunocytochemically during different stages of the estrous cycle. A fraction of granulosa cells of preantral and antral follicles exhibited a positive reaction to both antisera. So did endothelial cells of secretory and regressing corpora lutea. The staining was present throughout the stages of the estrous cycle. Liquid phase preadsorption with PGE2 and PGF2 alpha did not abolish the staining. However, it was absent after preadsorption with bovine serum albumin, the conjugating protein used for hapten immunization. The outcome of the preadsorption control demonstrates a classic example of a pitfall in immunostaining caused by the presence of contaminating antibodies.
D000818:Animals; D003338:Corpus Luteum / Q000032:analysis; D015237:Dinoprost; D015232:Dinoprostone; D004727:Endothelium / Q000032:analysis; D004971:Estrus; D005260:Female; D006107:Granulosa Cells / Q000032:analysis; D007106:Immune Sera / Q000276:immunology; D007150:Immunohistochemistry; D006080:Ovarian Follicle / Q000032:analysis; D010053:Ovary / Q000032:analysis*; D011458:Prostaglandins E / Q000032:analysis*; D011460:Prostaglandins F / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains
D007106:Immune Sera; D011458:Prostaglandins E; D011460:Prostaglandins F; D015237:Dinoprost; D015232:Dinoprostone