An ACCC-containing protein-binding sequence in the neighbourhood of the decanucleotide recognition site of the immunoglobulin gene promoter.
The decanucleotide (dc) box TNATTTGCAT is an important functional element in the K light chain gene promoter and was shown to bind nuclear proteins. We now describe the binding of nuclear factor III to dc containing oligonucleotides and define a second protein binding site in the neighbourhood of dc which turned out to be recognized by a partially purified NFIII preparation but not by highly purified NFIII (abbreviations ref. 1). This site comprises an ACCC-containing sequence element. It was further characterized by the hydroxyl radical footprinting method which is applicable to 40 bp long oligonucleotides. The significance of the ACCC-containing sequence and of the previously described 15mer sequence pd is discussed.
D000225:Adenine; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003596:Cytosine; D005803:Genes, Immunoglobulin; D007147:Immunoglobulin Light Chains / Q000235:genetics; D007145:Immunoglobulin kappa-Chains / Q000235:genetics; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000378:metabolism*; D009838:Oligodeoxyribonucleotides / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D011485:Protein Binding
D007147:Immunoglobulin Light Chains; D007145:Immunoglobulin kappa-Chains; D009687:Nuclear Proteins; D009838:Oligodeoxyribonucleotides; D003596:Cytosine; D000225:Adenine