title: Hyperosmolar opening of the blood-brain barrier in the energy-depleted rat brain. Part 1. Permeability studies.
abstract: A simple saline perfusion system was used to investigate the effects of hyperosmolar solutions of arabinose and mannitol upon the permeability of the blood-brain barrier. The small, polar molecule [14C]mannitol and the larger, visual marker Evans blue were used as indicators of barrier integrity in the perfused energy-depleted brain. One-minute perfusion of hyperosmolar solutions consistently opened the barrier suggesting that the mechanism of osmotic barrier opening is independent of energy-producing metabolism. The accumulation of radiolabel in the brain was expressed as the ratio of tissue to perfusate radioactivity (Rt/Rp) and, for cerebrum, this increased from a control value of 0.0022 +/- 0.0007 (mean +/- SEM; n = 4) to a value of 0.0124 +/- 0.0008 (n = 4) following 0.9 M arabinose and to 0.0495 +/- 0.0072 (n = 4) following 1.8 M arabinose. There was a significant reduction of water content of hyperosmolar perfused brains. These findings support the hypothesis that osmotic barrier opening is the result of the passive shrinkage of endothelial cells and the surrounding tissue.
mesh_terms: D000818:Animals; D001089:Arabinose / Q000378:metabolism; D001812:Blood-Brain Barrier; D001834:Body Water / Q000378:metabolism; D001921:Brain / Q000378:metabolism*; D002199:Capillary Permeability; D002250:Carbon Radioisotopes; D004734:Energy Metabolism; D005070:Evans Blue; D006982:Hypertonic Solutions; D008353:Mannitol / Q000378:metabolism; D009994:Osmolar Concentration; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D002250:Carbon Radioisotopes; D006982:Hypertonic Solutions; D008353:Mannitol; D005070:Evans Blue; D001089:Arabinose