[Integration and expression of the human dihydrofolate reductase gene in the Bacillus subtilis chromosome].
Integration of functionally active human dihydrofolate reductase (hDHFR) gene into the Bacillus subtilis chromosome was performed. The clones obtained contained 1 to 7 copies of hDHFR gene per chromosome equivalent and were resistant to trimethoprim. In cell lysates of such clones a protein with the molecular mass of hDHFR was detected. The hDHFR gene was stably maintained in all clones having this gene integrated into the bacterial chromosome, when grown under non-selective conditions.
D001412:Bacillus subtilis / Q000201:enzymology / Q000235:genetics* / Q000648:ultrastructure; D001435:Bacteriophages / Q000235:genetics; D002876:Chromosomes, Bacterial / Q000201:enzymology*; D003001:Cloning, Molecular / Q000379:methods; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005786:Gene Expression Regulation; D005796:Genes; D005822:Genetic Vectors; D006801:Humans; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics*; D014169:Transformation, Bacterial
D004269:DNA, Bacterial; D013762:Tetrahydrofolate Dehydrogenase
Integratsiia i ékspressiia gena digidrofolatreduktazy cheloveka v khromosome Bacillus subtilis.