Improved method of screening for aflatoxin with a coconut agar medium.
Nine isolates of Aspergillus flavus and Aspergillus parasiticus were screened for aflatoxin production on a coconut extract agar medium. Aflatoxin-producing colonies were detected under long-wave UV light (365 nm) by blue fluorescence on the reverse side after 2 to 5 days of growth. Aflatoxin production was verified by chemical analysis. Several types of shredded coconut available in the United States were tested and found to be satisfactory. No additives were required. Various parameters affecting the test were investigated.
D000348:Aflatoxins / Q000032:analysis* / Q000096:biosynthesis; D001230:Aspergillus / Q000378:metabolism*; D001231:Aspergillus flavus / Q000378:metabolism*; D003059:Cocos; D003470:Culture Media; D005453:Fluorescence; D009754:Nuts
D000348:Aflatoxins; D003470:Culture Media