Kinetic characterization of the acyl-enzyme mechanism for beta-lactamase I.
beta-Lactamase I catalyses the hydrolysis of penicillins by an acyl-enzyme mechanism. A procedure was developed for determining the rate constants for the acylation and deacylation steps for the good substrates benzylpenicillin and phenoxymethylpenicillin; this depends on determining the fraction of enzyme that is present as acyl-enzyme in the steady state.
D000215:Acylation; D001665:Binding Sites; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007700:Kinetics; D010400:Penicillin G / Q000378:metabolism; D010404:Penicillin V / Q000378:metabolism*; D010405:Penicillinase / Q000378:metabolism*
D010405:Penicillinase; D010400:Penicillin G; D010404:Penicillin V