title: A regional survey of the resistance to beta-lactam antibiotics in clinical isolates of (facultative) aerobic micro-organisms.
abstract: From 1980 to 1986 a regional survey was performed to investigate the susceptibility of beta-lactam antibiotics in clinical isolates of Escherichia coli, Proteus mirabilis, Klebsiella pneumoniae, Enterobacter cloacae and Pseudomonas aeruginosa in 6 hospitals located in the southern part of the Netherlands. Each year 300 to 400 strains were tested. The susceptibility to representatives of the older and the newer beta-lactam antibiotics was determined by a microbroth dilution assay using an inoculum size of 10(5) colony forming units (CFU)/ml in ISO-Sensitest broth (Oxoid). No significant differences in antibiotic sensitivity of the micro-organisms from the different hospitals were found. There was no evidence for an increase in antibiotic resistance of the strains during the sampling period. In contrast, there was a general tendency to an increase in antibiotic sensitivity.
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001420:Bacteria, Aerobic / Q000187:drug effects* / Q000302:isolation & purification; D001424:Bacterial Infections / Q000382:microbiology; D003430:Cross-Sectional Studies; D004352:Drug Resistance, Microbial; D004754:Enterobacter / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects; D006801:Humans; D007711:Klebsiella pneumoniae / Q000187:drug effects; D009426:Netherlands; D011513:Proteus mirabilis / Q000187:drug effects; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D047090:beta-Lactams
chemical_list: D000900:Anti-Bacterial Agents; D047090:beta-Lactams