Modification of the 6-hydroxydopamine technique for the correct determination of superoxide dismutase.
Heikkila & Cabbat (Anal. Biochem. 75, 356-362 (1976] have proposed the autoxidation of the 6-hydroxydopamine as a method to test superoxide dismutase activity in biological samples. This method has several advantages but in some instances leads to incorrect determinations. We present here a necessary modification of the method to avoid bias.
D000818:Animals; D002374:Catalase / Q000494:pharmacology; D002417:Cattle; D004912:Erythrocytes / Q000201:enzymology; D005347:Fibroblasts / Q000201:enzymology; D006454:Hemoglobins; D006801:Humans; D006892:Hydroxydopamines; D010084:Oxidation-Reduction; D016627:Oxidopamine; D013482:Superoxide Dismutase / Q000032:analysis*
D006454:Hemoglobins; D006892:Hydroxydopamines; D016627:Oxidopamine; D002374:Catalase; D013482:Superoxide Dismutase