Evaluation of free fatty acid kinetics during TPN feeding of healthy rats.
Free fatty acid (FFA) kinetics were evaluated in TPN-fed healthy rats using a single fatty acid tracer. Rats were divided into three groups according to the nonprotein energetic substrate infused: glucose (A), monoacetoacetin-glucose (B), and lipid emulsion-glucose (C). Fat kinetics were measured by continuous infusion of [1-14C]palmitate. Total FFA and individual FFA concentrations were measured and turnover and oxidation were determined for the total pool of FFA and for palmitate. Groups A and B were similar in all parameters. Group C had increased total and individual FFA concentrations. Group C appeared to have decreased total plasma FFA turnover and unchanged oxidation compared to groups A and B. Palmitate appeared to have a 400% increase in oxidation and a 50% increase in turnover for rats in Group C when compared to Groups A and B. It is concluded that a single tracer does not accurately reflect plasma FFA during TPN using lipid emulsion.
D000818:Animals; D001835:Body Weight; D005217:Fat Emulsions, Intravenous / Q000378:metabolism; D005230:Fatty Acids, Nonesterified / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D007700:Kinetics; D008297:Male; D009584:Nitrogen / Q000032:analysis; D010084:Oxidation-Reduction; D010169:Palmitic Acids / Q000378:metabolism*; D010289:Parenteral Nutrition, Total; D051381:Rats; D011919:Rats, Inbred Strains
D005217:Fat Emulsions, Intravenous; D005230:Fatty Acids, Nonesterified; D010169:Palmitic Acids; D005947:Glucose; D009584:Nitrogen