Effect of mitomycin-C and cyclophosphamide on the polytene chromosomes of Anopheles stephensi (Culicidae:Diptera).
Polytene chromosomes from the salivary glands of the mosquito, Anopheles stephensi, were used to evaluate the mutagenic effect of mitomycin-C and cyclophosphamide. Second instar larvae were treated with 1.4 micrograms/ml of mitomycin-C and 20.7 micrograms/ml of cyclophosphamide separately for 24 h. Polytene chromosome preparations were made from the salivary glands of 4th instar larvae. Mitomycin-C treated stocks showed as many as 46.99 +/- 2.48% aberrations compared with 7.5 +/- 0.80% in the controls. Similarly, cyclophosphamide treated larvae showed the aberrated cells to be 36.65 +/- 1.45% as against 7.93 +/- 0.52% in the controls. The results were statistically significant (P less than 0.001) for the two antitumour drugs. Both drugs are mutagenic to polytene chromosomes which are sensitive indicators of DNA damage.
D000818:Animals; D000852:Anopheles / Q000235:genetics; D002869:Chromosome Aberrations; D002875:Chromosomes / Q000187:drug effects*; D003520:Cyclophosphamide / Q000633:toxicity*; D016685:Mitomycin; D008937:Mitomycins / Q000633:toxicity*; D009152:Mutagenicity Tests / Q000379:methods
D008937:Mitomycins; D016685:Mitomycin; D003520:Cyclophosphamide