Acid-base changes in ischemic myocardium and intervention with hypothermia or bicarbonate.
Effects of ischemia and reperfusion on acid-base changes in relation to myocardial contractility, and the effects of correcting H+ were studied by lowering PCO2 or increasing HCO3- levels. The hearts were perfused by working heart mode and whole heart ischemia was induced by use of a one way valve followed by myocardial warming (37 degrees C, normothermia), cooling (18 degrees C, hypothermia) or warming plus 2.1 mM NaHCO3 for 15 min. The hearts were then reperfused for 20 min. Coronary effluent was collected through pulmonary artery cannulation and used for the measurement of acid-base changes. There were close correlations between the decrease in coronary flow and LV pressure, LV dP/dt. Close correlations were also observed between the decline in LV pressure and the rise in PCO2, H+, and the decline in HCO3-. A highly significant correlation was seen between H+ and lactate production. Myocardial contractility decreased to the same extent in 3 groups during ischemia, whereas its recovery rate in both the hypothermia and HCO3- -treated groups were significantly higher than in the normothermia group. The increment of H+ was significantly less in both the hypothermia and HCO3- -treated groups than in normothermia. These results indicate that lactate production is the major H+ producing source and the correction of H+ could minimize the ischemic insult and at the same time contribute to the reperfusion injury.
D000137:Acid-Base Imbalance / Q000209:etiology*; D000818:Animals; D001639:Bicarbonates / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism; D002245:Carbon Dioxide / Q000097:blood; D003326:Coronary Circulation; D003327:Coronary Disease / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007036:Hypothermia, Induced; D007773:Lactates / Q000378:metabolism; D019344:Lactic Acid; D009200:Myocardial Contraction; D009206:Myocardium / Q000378:metabolism; D015424:Reperfusion
D001639:Bicarbonates; D007773:Lactates; D002245:Carbon Dioxide; D019344:Lactic Acid; D002118:Calcium