Multiple effects of ethylmercurithiosalicylate on the metabolization of arachidonic acid by human neutrophils.
The effects of ethylmercurithiosalicylate (thimerosal) on the transformation of arachidonic acid via the 5-lipoxygenase pathway in human leukocytes stimulated with the Ca-ionophore A23187 were studied. Thimerosal inhibited acyltransferase, 5-lipoxygenase and the omega-oxidation system of LTB4 in a concentration-dependent fashion which was characteristic for the individual metabolites. LTA4 hydrolase activity was not affected. The inhibitory effects of thimerosal occurred instantaneously. The effects of the drug were not influenced by the concentration of the stimulus Ca-ionophore A23187.
D000217:Acyltransferases / Q000037:antagonists & inhibitors; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism*; D000001:Calcimycin / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D005035:Ethylmercury Compounds / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D016859:Lipoxygenase Inhibitors; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D013849:Thimerosal / Q000494:pharmacology*
D001095:Arachidonic Acids; D005035:Ethylmercury Compounds; D016859:Lipoxygenase Inhibitors; D013849:Thimerosal; D016718:Arachidonic Acid; D000001:Calcimycin; D000217:Acyltransferases