title: Detection of fos protein during osteogenesis by monoclonal antibodies.
abstract: We have generated monoclonal antibodies by using a synthetic peptide corresponding to amino acid positions 4 to 17 of the human fos protein. The antibodies detected both v- and c-fos proteins by immunoprecipitation, immunoblotting, and indirect immunofluorescence. The monoclonal antibodies not only identified the fos protein complex with the cellular 39-kilodalton protein, but also recognized the modified forms of the mouse, rat, and human fos proteins. In day-17 rat embryos, nuclear-staining fos protein could be identified in the cartilage by immunohistochemical staining.
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002356:Cartilage / Q000032:analysis / Q000196:embryology; D015683:Gene Products, gag; D006801:Humans; D010012:Osteogenesis; D011518:Proto-Oncogene Proteins / Q000032:analysis* / Q000276:immunology; D016760:Proto-Oncogene Proteins c-fos; D051381:Rats; D012191:Retroviridae Proteins / Q000032:analysis* / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D015683:Gene Products, gag; D011518:Proto-Oncogene Proteins; D016760:Proto-Oncogene Proteins c-fos; D012191:Retroviridae Proteins