title: Postsynthetic acetylation of histones during the cell cycle: a general function for the displacement of histones during chromatin rearrangements.
abstract: Postsynthetic acetylation of core histones exhibits a peak during S-phase of the Physarum cell cycle. The maximum 3H-acetate incorporation precedes the maximum of histone synthesis. Acetate is incorporated into all core histones during S-phase, but only into H2A and H2B during G2-period. Resolution of acetylated H4-subspecies reveals acetate incorporation into preexisting H4, but not into newly synthesized molecules during mitosis and early S-phase. In a protamine competition assay histones from S-phase chromatin are released at lower protamine concentrations as compared to the lower acetylated G2-chromatin. We demonstrate a preferential release of highly acetylated H4-subspecies at low protamine concentrations. Our results fit into a general model of the relationship between histone acetylation and chromatin assembly. According to this model acetylation of core histones would serve as a signal for displacement of histones from nucleosomes by modulating histone-protein or histone-DNA interactions. We propose that this mechanism operates during DNA-replication and transcription, as well as during other chromatin rearrangements.
mesh_terms: D000085:Acetates / Q000378:metabolism; D019342:Acetic Acid; D000107:Acetylation; D002453:Cell Cycle; D002843:Chromatin / Q000648:ultrastructure*; D006657:Histones / Q000235:genetics*; D008239:Lysine / Q000378:metabolism; D008938:Mitosis; D010804:Physarum / Q000166:cytology / Q000235:genetics*; D011499:Protein Processing, Post-Translational; D014316:Tritium
chemical_list: D000085:Acetates; D002843:Chromatin; D006657:Histones; D014316:Tritium; D008239:Lysine; D019342:Acetic Acid