Quantitative immunoassay of anti-La antibodies using purified recombinant La antigen.
A purified recombinant La fusion protein was tested in an enzyme-linked immunosorbent assay to quantitate anti-La responses. This protein contained the immunodominant region of the La molecule fused to beta-galactosidase. In solid-phase assays, recombinant La protein was solubilized in urea and bound to polystyrene wells without loss of immunoreactivity. The recombinant-based enzyme-linked immunosorbent assay proved to be a sensitive method for the detection of anti-La binding, and it accurately distinguished anti-La precipitin positive sera from normal sera.
D000906:Antibodies / Q000032:analysis*; D001324:Autoantigens / Q000276:immunology*; D001426:Bacterial Proteins / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000276:immunology; D006801:Humans; D012261:Ribonucleoproteins; D001616:beta-Galactosidase / Q000276:immunology; D000097585:SS-B Antigen
D000906:Antibodies; D001324:Autoantigens; D001426:Bacterial Proteins; D012261:Ribonucleoproteins; D001616:beta-Galactosidase; D000097585:SS-B Antigen