Role of stress in the initial injury stages of cell killing by altered intracellular calcium.
A variety of cultured cell types are effectively killed by Ca2+-carrying ionophores (A23187, ionomycin and lasalocid) in the presence of adequate extracellular concentrations of Ca2+ and Na+, although cell killing mechanisms independent of these ions also exist. Previous studies identified 2 injury stages (termed stage I and stage II injury) at which the A23187-induced killing process in 3T3 fibroblasts is interrupted in the presence of low extracellular Ca2+ concentrations and in the absence of extracellular Na+, respectively. The present studies confirm the generality of stage II injury in Ca2+-mediated cell killing, but demonstrate a requirement for concomitant stress conditions (e.g., osmotic or oxidative stress) for expression of stage I injury.
D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000502:physiology*; D002470:Cell Survival; D002478:Cells, Cultured; D004987:Ethers / Q000494:pharmacology; D015759:Ionomycin; D007832:Lasalocid / Q000494:pharmacology; D009994:Osmolar Concentration; D010084:Oxidation-Reduction; D010740:Phospholipases / Q000037:antagonists & inhibitors
D001095:Arachidonic Acids; D004987:Ethers; D016718:Arachidonic Acid; D000001:Calcimycin; D015759:Ionomycin; D010740:Phospholipases; D002118:Calcium; D007832:Lasalocid