title: Absence of transepithelial anion exchange by rabbit OMCD: evidence against reversal of cell polarity.
abstract: In the rabbit cortical collecting duct (CCD), Cl tracer crosses the epithelium predominantly via an anion exchange system that operates in either a Cl-Cl or Cl-HCO3 exchange mode. In the present study, we used the 36Cl lumen-to-bath rate coefficient (KCl, nm/s), a sensitive measurement of CCD transepithelial anion transport, to investigate the nature of Cl transport in the medullary collecting duct dissected from inner stripe, outer medulla (OMCD). The KCl in OMCD perfused and bathed in HCO3-Ringer solution was low (46.2 +/- 8.5 nm/s) and similar to that value observed in the CCD when anion exchange is inhibited and Cl permeates the epithelium by diffusion. Unlike KCl in CCD, KCl in OMCD was not stimulated by adenosine 3',5'-cyclic monophosphate (cAMP). OMCD KCl was not altered by bath Cl and/or HCO3 removal, demonstrating the absence of transepithelial Cl-Cl and Cl-HCO3 exchange. To test the hypothesis that metabolic alkalosis could reverse the polarity of intercalated cells and thus induce an apical Cl-HCO3 exchanger in H+-secreting OMCD cells, we measured KCl in OMCD from rabbits made alkalotic by deoxycorticosterone and furosemide. Although the base-line KCl was slightly higher than in OMCD from control rabbits, the value was still far lower than the KCl under comparable conditions in CCD. Moreover, KCl in OMCD from alkalotic rabbits was unchanged by cAMP, or by sequential removal of bath HCO3 and Cl. Immunocytochemistry using peanut lectin and a monoclonal antibody to-erythrocyte band 3 failed to reveal any evidence for alkalosis-induced reversal of either CCD or OMCD intercalated cell polarity.(ABSTRACT TRUNCATED AT 250 WORDS)
mesh_terms: D000471:Alkalosis / Q000503:physiopathology; D000818:Animals; D000838:Anions; D002245:Carbon Dioxide / Q000097:blood; D002712:Chlorides / Q000378:metabolism*; D004573:Electrolytes / Q000097:blood; D004848:Epithelium / Q000502:physiology; D066298:In Vitro Techniques; D007672:Kidney Cortex / Q000502:physiology* / Q000503:physiopathology; D007679:Kidney Medulla / Q000502:physiology* / Q000503:physiopathology; D007684:Kidney Tubules / Q000502:physiology*; D007685:Kidney Tubules, Collecting / Q000502:physiology* / Q000503:physiopathology; D010100:Oxygen / Q000097:blood; D010313:Partial Pressure; D011817:Rabbits; D012016:Reference Values
chemical_list: D000838:Anions; D002712:Chlorides; D004573:Electrolytes; D002245:Carbon Dioxide; D010100:Oxygen