[Determination of aflatoxin B1 in dried figs by visual screening, thin-layer chromatography and ELISA].
Aflatoxin B1-contaminated fruits were sorted out from 250 kg dried figs (five Turkish and three Greek batches) by bright-greenish-yellow fluorescence under UV light. The aflatoxins of the fluorescent figs were extracted by simple soaking in methanol. Aflatoxin B1 was determined by thin-layer chromatography. Parallel to this, an extraction for the determination of aflatoxin B1 was developed by a competitive ELISA and the two methods were compared with each other. In a highly contaminated batch of Turkish figs, statistically there was one fig among 350 which had a high aflatoxin content (greater than 100 ng/g fig) and one fig amongst 140 fruits with an aflatoxin B1 content of greater than 10 ng B1/g fig.
D016604:Aflatoxin B1; D000348:Aflatoxins / Q000032:analysis*; D002855:Chromatography, Thin Layer / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005453:Fluorescence; D005519:Food Preservation; D005638:Fruit / Q000032:analysis*; D014466:Ultraviolet Rays
D000348:Aflatoxins; D016604:Aflatoxin B1
Bestimmung von Aflatoxin B1 in Trockenfeigen durch visuelles Screening, Dünnschichtchromatographie und ELISA.