Metavinculin and vinculin from mammalian smooth muscle: bulk isolation and characterization.
Metavinculin, a vinculin related protein found only in muscle, has been prepared in bulk amounts from porcine stomach by a new procedure: the same procedure is applicable to the purification of vinculin from porcine stomach and avian gizzard. A comparison of the mammalian and avian proteins by peptide mapping showed them all to contain a common protease resistant 90 kDa core; however both avian and mammalian vinculins were notably more resistant to proteolysis down to this core than their respective metavinculins. Despite the close similarities in the peptide maps, in molecular weight and amino acid composition neither of the mammalian proteins exhibited the head and tail morphology formerly described for gizzard vinculin and metavinculin; both porcine proteins appeared globular under the electron microscope. From the gross variability in the ratios of metavinculin to vinculin among smooth muscles of different origin as well as from the common detergent-independent solubility properties of both proteins during isolation, it is concluded that vinculin and metavinculin perform duplicatory roles as peripheral membrane components. No definitive evidence for the interaction of either protein with actin filaments was obtained.
D000818:Animals; D030161:Avian Proteins; D002148:Calmodulin-Binding Proteins / Q000032:analysis; D007158:Immunologic Techniques; D007526:Isoelectric Point; D008854:Microscopy, Electron; D008970:Molecular Weight; D009124:Muscle Proteins / Q000302:isolation & purification*; D009130:Muscle, Smooth / Q000032:analysis*; D010449:Peptide Mapping; D011487:Protein Conformation; D013270:Stomach; D013552:Swine; D016596:Vinculin
D030161:Avian Proteins; D002148:Calmodulin-Binding Proteins; D009124:Muscle Proteins; C035689:VCL protein, Gallus gallus; D016596:Vinculin