Role of interleukin 2, interleukin 4, and alpha, beta, and gamma interferon in stimulating macrophage antibody-dependent tumoricidal activity.
Pretreatment of murine peritoneal exudate macrophages with 1-5 U/ml rIFN-gamma or rIL-2, or higher concentrations of IFN-alpha or IFN-beta greatly stimulated ADCC to Rl lymphoma targets. The assay was direct counting of viable target cells after 9 and 24 h using an E/T ratio of 5:1. 2d of pretreatment was optimal for enhancing ADCC. rIL-4 was inactive and IL-4-depleted Con A-induced spleen lymphokine retained its ADCC-stimulating activity. Antibody to IFN-gamma blocked the ADCC-promoting effect of the lymphokine, suggesting a major role for this factor.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000502:physiology; D000920:Antibody-Dependent Cell Cytotoxicity / Q000187:drug effects*; D001666:Binding Sites, Antibody; D004306:Dose-Response Relationship, Immunologic; D007370:Interferon Type I / Q000502:physiology*; D007371:Interferon-gamma / Q000276:immunology / Q000502:physiology*; D007376:Interleukin-2 / Q000502:physiology*; D015847:Interleukin-4; D007378:Interleukins / Q000502:physiology*; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D008809:Mice, Inbred C3H; D013945:Thymoma / Q000276:immunology
D000911:Antibodies, Monoclonal; D007370:Interferon Type I; D007376:Interleukin-2; D007378:Interleukins; D015847:Interleukin-4; D007371:Interferon-gamma