Carbon-14-ochratoxin A distribution in the Japanese quail (Coturnix coturnix japonica) monitored by whole body autoradiography.
Tissue distribution of the nephrotoxic mycotoxin ochratoxin A was characterized in laying Japanese quail by whole body autoradiography and scintillation counting using 14C-labelled toxin. Periodically for 8 days after one intravenous injection of 14 microCi/bird, corresponding to 70 ng/g body weight, birds were killed, frozen, and sagittal sections of the whole body were placed on X-ray film. In general, the ochratoxin disappeared from the avian body rapidly. Specific retention of radioactivity was seen as a ring-like distribution in yolks and growing follicles. After sectioning, organs and intestinal contents were removed from carcasses in a frozen condition, homogenized, extracted, chromatographed, and the radioactivity in fractions was measured by scintillation spectroscopy. High concentrations of ochratoxin A were found in gastric intestinal contents, probably originating from toxin excreted in the bile.
D000818:Animals; D001345:Autoradiography; D003370:Coturnix / Q000378:metabolism*; D005260:Female; D009793:Ochratoxins / Q000032:analysis / Q000493:pharmacokinetics*; D011784:Quail / Q000378:metabolism*; D014018:Tissue Distribution; D014915:Whole-Body Counting
D009793:Ochratoxins; C025589:ochratoxin A