Effect of antiprogestin ZK 98.734 on the ovarian cycle, early pregnancy, and on its binding to progesterone receptors in the myometrium of marmoset Callithrix jacchus.
The antiprogestin ZK 98.734 (11 beta-(4-dimethylaminophenyl-17 beta-hydroxy-17 alpha-(3-hydroxy-prop-1(Z)-enyl-4,9(10)-estradien-3-one) was administered i.m. (5 mg/day) for three consecutive days to two groups of common marmosets. In one group (nonpregnant, n = 6), it was injected during the luteal phase, and to the second group (pregnant, n = 7), it was injected during early pregnancy, on Days 24-26 of the mid-cycle estradiol peak. Administration of ZK 98.734 during the luteal phase caused a sharp drop in plasma progesterone levels. The luteal phase was shortened whether the drug was administered during the early or the late luteal phase. Similarly, administration of ZK 98.734 during early pregnancy caused a significant drop in progesterone levels, and pregnancy was terminated in all of the animals. The post-treatment cycles in both groups of animals were ovulatory and of normal duration. 3H-ZK 98.734 showed specific binding to myometrial cytosol fraction. ZK 98.734 also displaced the binding of 3H-progesterone to progesterone receptors. However, progesterone had higher binding affinity than did ZK 98.734. The antifertility action of ZK 98.734 could be a result either of its luteolytic action or of its blocking the progesterone receptors in the target tissue. This study, therefore, indicates that in the common marmoset ZK 98.734 is a progesterone antagonist with a potential to terminate early pregnancy.
D000818:Animals; D002143:Callitrichinae; D003338:Corpus Luteum / Q000187:drug effects; D003600:Cytosol / Q000378:metabolism; D004963:Estrenes / Q000378:metabolism / Q000494:pharmacology*; D005260:Female; D008597:Menstrual Cycle / Q000187:drug effects*; D009215:Myometrium / Q000187:drug effects*; D010052:Ovariectomy; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000187:drug effects*; D011374:Progesterone / Q000037:antagonists & inhibitors / Q000097:blood; D011863:Radioimmunoassay; D011980:Receptors, Progesterone / Q000378:metabolism*; D014316:Tritium
D004963:Estrenes; D011980:Receptors, Progesterone; D014316:Tritium; C048260:lilopristone; D011374:Progesterone