title: Distinct synthetic and structural characteristics of proteoglycans produced by cultured artery smooth muscle cells of atherosclerosis-susceptible pigeons.
abstract: Proteoglycan (PG) metabolism by aortic smooth muscle cell cultures derived from atherosclerosis-susceptible White Carneau (WC) and -resistant Show Racer (SR) pigeons was compared using [35S]sodium sulfate and [3H]serine or [3H]glucosamine as labeling precursors. Chondroitin sulfate (CS) PG and dermatan sulfate (DS) PG were the major PG secreted into the medium by both cell types. Total PG production, whether measured by incorporation of radiolabel into either core protein or glycosaminoglycan chains, was consistently lower in WC compared to SR cultures at several time points. This difference was due in part to lower (30-37%) PG synthesis in WC cells, but degradation of newly synthesized PG was an important contributor. A pulse-chase study indicated that of the total radiolabeled PG present at time O, only 47% was present at 24 h in WC cultures compared to 88% in SR cultures. The large CS-PG appeared to be the primary target for degradation in WC cells, and this selective processing resulted in a higher DS-PG:CS-PG ratio in these cultures. Structural studies indicated similar core protein and glycosaminoglycan chain sizes within a PG type for both cell types. PG monomer composition differed, however, by a higher sulfation of WC CS-PG compared to SR CS-PG and by a disaccharide sulfation position favoring 6-sulfation in WC PG and 4-sulfation in SR PG.
mesh_terms: D000818:Animals; D001011:Aorta; D001161:Arteriosclerosis / Q000378:metabolism*; D002478:Cells, Cultured; D011232:Chemical Precipitation; D002809:Chondroitin Sulfates / Q000096:biosynthesis; D002845:Chromatography; D010856:Columbidae / Q000378:metabolism*; D003470:Culture Media; D003871:Dermatan Sulfate / Q000096:biosynthesis; D004187:Disaccharides / Q000032:analysis; D005944:Glucosamine / Q000378:metabolism; D006025:Glycosaminoglycans / Q000032:analysis / Q000378:metabolism; D007700:Kinetics; D009131:Muscle, Smooth, Vascular / Q000378:metabolism*; D011509:Proteoglycans / Q000302:isolation & purification / Q000378:metabolism*; D012694:Serine / Q000378:metabolism; D013431:Sulfates / Q000378:metabolism
chemical_list: D003470:Culture Media; D004187:Disaccharides; D006025:Glycosaminoglycans; D011509:Proteoglycans; D013431:Sulfates; C012036:sodium sulfate; D003871:Dermatan Sulfate; D012694:Serine; D002809:Chondroitin Sulfates; D005944:Glucosamine