[Cloning and nucleotide sequence analysis of the cDNA of the immunoglobulin lambda light chain from the bovine udder].
A cDNA library was constructed from the mRNA of bovine mammary gland which contained Ig lambda producing plasmacytes. Overlapping clones encompassing the major portion of the coding sequence of the Ig lambda mRNA were isolated and sequenced. Predicted amino acid sequence shows a mature polypeptide of 217 residues in which V, J and C regions can be distinguished. The constant region has greatest homology with human Ig lambda mRNA constant region. The area of the V region between CDR3 and CDR2 has also great homology with the same area of human lambda chain.
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D004274:DNA, Recombinant; D005260:Female; D005815:Genetic Code; D006801:Humans; D007146:Immunoglobulin lambda-Chains / Q000235:genetics*; D008321:Mammary Glands, Animal / Q000276:immunology*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid
D004274:DNA, Recombinant; D007146:Immunoglobulin lambda-Chains; D012333:RNA, Messenger; D004247:DNA
Klonirovanie i analiz nukleotidnoĭ posledovatel'nosti kDNK legkoĭ lambda-tsepi immunoglobulina iz molochnoĭ zhelezy korovy.