Role of the time factor in the development of hormonal imprinting of insulin receptors in Chang liver cell cultures.
Chang liver cells were pretreated with insulin for 5, 10, 20 and 60 minutes, and 4 and 24 hours after the treatment the binding of FITC-labelled hormone was measured. Hormone binding decreased after 5 minutes treatment, while longer treatments the binding increased considerably. The binding of hormone, at 4 hours investigation, to the nuclear membrane increased more markedly than in plasma membrane, indicating the important role of the imprinting of nuclear membrane. At 24 hours, the increased binding capacity is more characteristic for the plasma membrane.
D000818:Animals; D002460:Cell Line; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D005456:Fluorescent Dyes; D007328:Insulin / Q000378:metabolism / Q000494:pharmacology*; D007700:Kinetics; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D011972:Receptor, Insulin / Q000096:biosynthesis* / Q000378:metabolism; D013861:Thiocyanates
D005452:Fluoresceins; D005456:Fluorescent Dyes; D007328:Insulin; D013861:Thiocyanates; D011972:Receptor, Insulin; D016650:Fluorescein-5-isothiocyanate