title: Cellular aspects of pre-ovulatory folliculogenesis in primate ovaries.
abstract: According to current concepts of pre-ovulatory folliculogenesis in primate ovaries, each growing follicle has a 'threshold' requirement for stimulation by FSH which must be met if it is to enter the oestrogen-secretory phase of pre-ovulatory development. Until recently, our understanding of the intra-follicular mechanisms underlying FSH action on granulosa cells was based largely on information from non-primate laboratory animals, mainly polyovulators such as rats. The present paper describes studies on FSH-regulated granulosa cell function in relation to pre-ovulatory development in vivo and in vitro using a laboratory primate, the common marmoset (Callithrix jacchus). Measurement of aromatase activity is used as an index of granulosa cell cytodifferentiation to verify three major tenets of the 'threshold' hypothesis: (i) that granulosa cells acquire increased responsiveness to FSH and LH during pre-ovulatory growth; (ii) that these developmentally related changes are directly induced by FSH; and (iii) that intrafollicular steroids modify FSH action and thereby contribute to the establishment of follicular FSH thresholds. The results obtained highlight the value of this experimental animal model for studies of cellular and molecular aspects of pre-ovulatory folliculogenesis which are relevant to human ovaries.
mesh_terms: D000818:Animals; D001141:Aromatase / Q000378:metabolism; D002143:Callitrichinae / Q000502:physiology*; D002453:Cell Cycle; D002454:Cell Differentiation; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D005260:Female; D005640:Follicle Stimulating Hormone / Q000502:physiology; D006107:Granulosa Cells / Q000187:drug effects / Q000201:enzymology; D007986:Luteinizing Hormone / Q000494:pharmacology; D008597:Menstrual Cycle; D006080:Ovarian Follicle / Q000254:growth & development*; D010053:Ovary / Q000166:cytology*
chemical_list: D006063:Chorionic Gonadotropin; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D001141:Aromatase