Purification of ribonuclease T1.
An improved method for purifying ribonuclease T1 from Aspergillus oryzae is described. The method uses gradient elution from DEAE-cellulose and sulfopropyl-Sephadex columns followed by gel filtration on Sephadex G-50 to give almost 100 mg (50% yield) of ribonuclease T1 from 100 g of starting material in less than 5 days.
D001236:Aspergillus oryzae / Q000201:enzymology; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D004722:Endoribonucleases / Q000302:isolation & purification*; D006163:Ribonuclease T1 / Q000302:isolation & purification*; D013053:Spectrophotometry
D004722:Endoribonucleases; D006163:Ribonuclease T1