Determination of methylated amino acids in human serum by high-performance liquid chromatography.
Employing high-performance liquid chromatography with isocratic elution using a two-column system (mu Bondapak C18 and mu Bondapak CN) and phenyl isothiocyanate as a fluorogenic reagent, NG-monomethylarginine (MMA). NG-dimethylarginines, and epsilon-N-trimethyllysine (TML) can be quatitatively separated from human serum samples. The recoveries of these amino acids were over 90%. It was observed that the serum concentrations of MMA, DMA and TML were significantly elevated in sera obtained from patients suffering from diabetes mellitus, hepatitis or hyperthyroidism, particularly the last condition.
D000596:Amino Acids / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D006980:Hyperthyroidism / Q000097:blood; D008239:Lysine / Q000032:analysis; D008745:Methylation; D009764:o-Phthalaldehyde
D000596:Amino Acids; D009764:o-Phthalaldehyde; D008239:Lysine