Immunohistochemical study of alkaline phosphatase in growth plate cartilage, bone, and fetal calf isolated chondrocytes using monoclonal antibodies.
Monoclonal antibodies prepared against alkaline phosphatase of matrix vesicles from fetal bovine growth plate cartilage were used to study the distribution of alkaline phosphatase in bovine bone and cartilage. Primary chondrocyte cultures from bovine growth plate cartilage were prepared and expression of this antigen was followed during 3 d long cultured period. Same monoclonal anti-alkaline phosphatase antibodies recognized both osteoblasts in metaphyseal bone as well as proliferating and hypertrophic chondrocytes in growth plate cartilage. Extracellular reaction was observed in lower areas of growth plate cartilage after hyaluronidase digestion of tissue sections. In isolated growth plate chondrocytes strong membrane associated reaction as well as weaker intracytoplasmic reaction were noticed and especially in large hypertrophic cells membrane reaction was clustered showing "capping-like" phenomenon. These results indicates the high similarity of alkaline phosphatase in bone and cartilage and its uneven distribution in chondrocyte plasma membrane.
D000469:Alkaline Phosphatase / Q000032:analysis* / Q000276:immunology; D000818:Animals; D000911:Antibodies, Monoclonal; D001842:Bone and Bones / Q000201:enzymology*; D002356:Cartilage / Q000166:cytology / Q000201:enzymology*; D002417:Cattle; D005333:Fetus; D019793:Fluorescein; D005452:Fluoresceins; D006132:Growth Plate / Q000201:enzymology*; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D010006:Osteoblasts / Q000201:enzymology
D000911:Antibodies, Monoclonal; D005452:Fluoresceins; D000469:Alkaline Phosphatase; D019793:Fluorescein