Interactions of the anti-tumor ametantrone and mitoxantrone with rat hepatic microsomes.
The interaction of the anti-tumor anthraquinones, ametantrone and mitoxantrone, with rat hepatic microsomes has been studied with a fluorescence technique using 7,12-dimethylbenzanthracene as a new fluorescent probe. The two drugs were able to quench the intrinsic fluorescence of microsomal suspension. Mitoxantrone was able to displace dimethylbenzanthracene bound microsomes with a linear representation of one ligand-one acceptor model, whereas bimodal shape was found in the case of ametantrone. The mechanism of quenching and/or binding is discussed.
D015127:9,10-Dimethyl-1,2-benzanthracene / Q000378:metabolism; D000818:Animals; D000970:Antineoplastic Agents / Q000378:metabolism*; D005453:Fluorescence; D066298:In Vitro Techniques; D008862:Microsomes, Liver / Q000378:metabolism*; D008942:Mitoxantrone / Q000031:analogs & derivatives* / Q000378:metabolism*; D011485:Protein Binding; D051381:Rats; D011919:Rats, Inbred Strains
D000970:Antineoplastic Agents; D015127:9,10-Dimethyl-1,2-benzanthracene; D008942:Mitoxantrone; C020182:ametantrone