title: Effects of incomplete ischemia and subsequent recirculation on free palmitate, stearate, oleate and arachidonate levels in lumbar and cervical spinal cord of rabbit.
abstract: The effect of severe incomplete ischemia, induced by abdominal aorta ligation for 40 minutes, and subsequent recirculation for one and four days on accumulation of free fatty acids was studies in the lumbar and cervical part of rabbit spinal cord. Changes in free fatty acid levels were determined separately in gracile fascicle (Fg), dorsal part (Dp, without Fg) and ventral part (Vp) of both spinal cord regions. In lumbar spinal cord increases in free fatty acid levels, especially that of arachidonate, were observed in Fg, Dp and Vp a the end of the ischemic period. During recirculation all values were similar to nonischemic controls. In cervical spinal cord a slight increase in free fatty acid levels was found in Fg after four days of recirculation, and in Dp arachidonate and stearate levels were most markedly elevated after one day of recirculation. No changes at any interval were found in Vp of cervical spinal cord. The present results indicate that the experimental insult induced typical ischemic injury to spinal cord tissue demonstrated by fatty acid liberation from membrane lipids. This injury may affect neurotransmission and other processes and free fatty acids themselves impair tissue metabolism (inhibition of oxidative phosphorylation, edema precipitation, synthesis of eicosanoids) and thus restrict the possibilities to enhance recovery in the recirculation period.
mesh_terms: D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000032:analysis*; D005260:Female; D007511:Ischemia / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D019301:Oleic Acid; D009829:Oleic Acids / Q000032:analysis*; D009928:Organ Specificity; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000032:analysis*; D011817:Rabbits; D013116:Spinal Cord / Q000032:analysis / Q000098:blood supply*; D013229:Stearic Acids / Q000032:analysis*
chemical_list: D001095:Arachidonic Acids; D009829:Oleic Acids; D010169:Palmitic Acids; D013229:Stearic Acids; D016718:Arachidonic Acid; D019301:Oleic Acid; D019308:Palmitic Acid; C031183:stearic acid