High affinity human monoclonal antibodies directed against hepatitis B surface antigen.
Peripheral blood mononuclear cells from donors immunized with hepatitis B vaccine (Pasteur Hevac B) were transformed with Epstein-Barr virus. Two polyclonal cell lines, producing antibodies to hepatitis B surface antigen were established and cloned. Seven clones were isolated; they secreted between 10 and 20 micrograms/ml of HBs specific IgG1 kappa or lambda antibody with anti-HBs titer of 300-800 IU/ml. These human antibodies expressed the anti 'a' specificities and had high affinity and avidity; their potential use as reagents for hepatitis B virus detection and for passive immunotherapy is under study.
D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D002461:Cell Line, Transformed; D002999:Clone Cells; D006509:Hepatitis B / Q000276:immunology; D006510:Hepatitis B Antibodies / Q000276:immunology*; D006514:Hepatitis B Surface Antigens / Q000032:analysis / Q000276:immunology*; D006801:Humans; D007118:Immunoassay; D005779:Immunodiffusion; D007525:Isoelectric Focusing; D008099:Liver / Q000276:immunology
D000911:Antibodies, Monoclonal; D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens