Serotyping of Pseudomonas aeruginosa strains isolated in Bulgaria using the Lányi-Bergan combined scheme.
Two hundred Pseudomonas aeruginosa strains isolated in hospitals in Bulgaria were serotyped according to the combined scheme of Lányi and Bergan, supplemented by Akatova and Smirnova and Homma, using agglutinating O-antisera prepared in the National Institute of Hygiene, Budapest. The most frequently encountered serogroup is O2 (29%) followed by O11 (28.5%), O6, O3, O10 etc. The results were compared with those obtained by using Difco antisera prepared according to Liu et al., and showed 96.5% coincidence. The strains were phage typed according to the scheme of Meitert and tested for antibiotic resistance to aminoglycosides (gentamicin, carbenicillin, tobramycin and amikacin). Phage groups 3 (3a and 3(3)) and 1 (1a) predominated. The strains exhibited sensitivity to amikacin (99%) and frequent resistance to gentamicin (45.8%, carbenicillin (40%) and tobramycin (28%). Subdivision of the serogroups into phage and resisto-types contributes to analysis of nosocomial infections.
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001434:Bacteriophage Typing; D002031:Bulgaria; D004352:Drug Resistance, Microbial; D006761:Hospitals; D006801:Humans; D011550:Pseudomonas aeruginosa / Q000145:classification* / Q000187:drug effects; D012703:Serotyping
D000900:Anti-Bacterial Agents