A point mutation in the last intron responsible for increased expression and transforming activity of the c-Ha-ras oncogene.
The T24/EJ allele of the Ha-ras proto-oncogene owes its powerful oncogenic activity not merely to the well documented mutation that perturbs the structure of the encoded polypeptide, but in addition to a second single nucleotide alteration in an intron that causes a tenfold increase in expression. This effect on expression is maintained upon transfer of the surrounding DNA to a heterologous gene, and as such defines a novel regulatory element.
D000818:Animals; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D019204:GTP-Binding Proteins / Q000502:physiology; D005786:Gene Expression Regulation; D006801:Humans; D007438:Introns; D009154:Mutation; D009857:Oncogenes; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D051381:Rats; D012045:Regulatory Sequences, Nucleic Acid
C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins; D019204:GTP-Binding Proteins