The modulation of serine metabolism in hepatoma 3924A during different phases of cellular proliferation in culture.
The activities of 3-phosphoglycerate dehydrogenase and serine hydroxymethyltransferase increased markedly during the transition of hepatoma cells from a resting non-proliferating culture into the proliferating growth phase. Activities declined as cells reached confluency and entered the plateau growth phase. This pattern was paralleled by changes in [14C]serine incorporation into nucleic acids. The experiments support the hypothesis that the biosynthesis of serine is metabolically coupled to its utilization for nucleotide precursor formation in cancer cells.
D000818:Animals; D002237:Carbohydrate Dehydrogenases / Q000378:metabolism; D002452:Cell Count; D002455:Cell Division; D002460:Cell Line; D012696:Glycine Hydroxymethyltransferase / Q000378:metabolism; D008114:Liver Neoplasms, Experimental / Q000378:metabolism*; D009696:Nucleic Acids / Q000378:metabolism; D050543:Phosphoglycerate Dehydrogenase; D012694:Serine / Q000378:metabolism*
D009696:Nucleic Acids; D012694:Serine; D002237:Carbohydrate Dehydrogenases; D050543:Phosphoglycerate Dehydrogenase; D012696:Glycine Hydroxymethyltransferase