The calcium-mobilizing action of low concentrations of sodium fluoride in single fibroblasts.
The sensitivity of fibroblasts (L cells) to low concentrations of sodium fluoride (NaF) was examined using the same cell during a series of stimuli. NaF with increasing concentrations up to 1 mM elevated cytosolic free Ca2+ concentrations ([Ca2+]i) in single cells. [Ca2+]i increased within 15 sec of addition of NaF and lowered to basal [Ca2+]i levels quickly. This elevation was observed both in the presence and absence of external Ca2+ and was enhanced by 1 microM Al3+. These results suggest that low concentrations (below 1 mM) of NaF induce Ca2+ release from intracellular Ca2+ stores in single L cells through guanine nucleotide-binding protein (G protein).
D000535:Aluminum / Q000378:metabolism; D001572:Benzofurans; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D005347:Fibroblasts / Q000378:metabolism*; D016257:Fura-2; D019204:GTP-Binding Proteins / Q000378:metabolism; D006801:Humans; D012969:Sodium Fluoride / Q000378:metabolism*
D001572:Benzofurans; D012969:Sodium Fluoride; D000535:Aluminum; D019204:GTP-Binding Proteins; D002118:Calcium; D016257:Fura-2