title: The calcium-mobilizing action of low concentrations of sodium fluoride in single fibroblasts.
abstract: The sensitivity of fibroblasts (L cells) to low concentrations of sodium fluoride (NaF) was examined using the same cell during a series of stimuli. NaF with increasing concentrations up to 1 mM elevated cytosolic free Ca2+ concentrations ([Ca2+]i) in single cells. [Ca2+]i increased within 15 sec of addition of NaF and lowered to basal [Ca2+]i levels quickly. This elevation was observed both in the presence and absence of external Ca2+ and was enhanced by 1 microM Al3+. These results suggest that low concentrations (below 1 mM) of NaF induce Ca2+ release from intracellular Ca2+ stores in single L cells through guanine nucleotide-binding protein (G protein).
mesh_terms: D000535:Aluminum / Q000378:metabolism; D001572:Benzofurans; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D005347:Fibroblasts / Q000378:metabolism*; D016257:Fura-2; D019204:GTP-Binding Proteins / Q000378:metabolism; D006801:Humans; D012969:Sodium Fluoride / Q000378:metabolism*
chemical_list: D001572:Benzofurans; D012969:Sodium Fluoride; D000535:Aluminum; D019204:GTP-Binding Proteins; D002118:Calcium; D016257:Fura-2