Studies on the procurement of blood coagulation factor VIII. In vitro studies on blood components prepared in half-strength citrate anticoagulant 18 hours after phlebotomy.
Our previous studies have shown that the use of half-strength citrate anticoagulant improves plasma factor VIII stability while maintaining the viability of red cells and platelets. This study extends these observations to show that the stability of factor VIII is maintained in blood stored at room temperature or 4 degrees C overnight and that in vitro tests indicate comparable quality of red cells and platelets prepared from half-strength citrate donations processed immediately or after overnight storage at room temperature. The exception to this was a more rapid loss of red cell 2,3-diphosphoglycerate, as has previously been observed for full-strength citrate donations.
D001792:Blood Platelets / Q000032:analysis; D001793:Blood Preservation / Q000379:methods*; D002951:Citrates; D004912:Erythrocytes / Q000032:analysis; D005169:Factor VIII / Q000032:analysis*; D006801:Humans
D002951:Citrates; D005169:Factor VIII