Formation of lipoxins by alveolar macrophages.
Rat alveolar macrophages incubated with (15S), 15-hydroperoxy-5,8, 11-cis-13-trans-eicosatetraenoic acid (15-HPETE) in the presence of the calcium ionophore A23187 produced a series of polar compounds. Reverse-phase high performance liquid chromatography (RP-HPLC) was used to purify these compounds and their identities were characterized by physical criteria (UV spectroscopy, GC/MS) and by co-elution with synthetic compounds. Alveolar macrophage-derived materials proved to be lipoxin A4 [LXA4; (5S, 6R, 15S)-5,6,15-trihydroxy-7,9,13-trans-11-cis-eicosatetraenoic acid], lipoxin B4[LXB4; (5S, 14R, 15S)-5,14,15-trihydroxy-6,10,12-trans-8-cis- eicosatetraenoic acid], and a structural isomer. These results indicate that rat alveolar macrophages have the capacity to generate lipoxins and related compounds.
D000818:Animals; D001095:Arachidonic Acids / Q000378:metabolism*; D000001:Calcimycin / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D008401:Gas Chromatography-Mass Spectrometry; D006893:Hydroxyeicosatetraenoic Acids / Q000096:biosynthesis* / Q000302:isolation & purification; D066298:In Vitro Techniques; D015289:Leukotrienes; D008054:Lipid Peroxides / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013056:Spectrophotometry, Ultraviolet
D001095:Arachidonic Acids; D006893:Hydroxyeicosatetraenoic Acids; D015289:Leukotrienes; D008054:Lipid Peroxides; D000001:Calcimycin; C025086:15-hydroperoxy-5,8,11,13-eicosatetraenoic acid