title: Nitrosamine formation by denitrifying and non-denitrifying bacteria: implication of nitrite reductase and nitrate reductase in nitrosation catalysis.
abstract: Biochemical, microbiological and genetic studies were done to characterize the mechanism of bacterial formation of N-nitrosomorpholine (NMOR) from morpholine and nitrite at neutral pH. In Escherichia coli and Proteus morganii, the nitrosating activity was markedly induced when bacteria were cultured under anaerobiosis in minimal medium containing nitrate, while in the presence of nitrite there was no induction. However, induction of the nitrosating activity in Pseudomonas aeruginosa occurred in anaerobic cultures in the presence of either nitrate or nitrite. The nitrosation capacity was also examined in various E. coli K12 mutants whose structural gene of either nitrate reductase or nitrite reductase was deleted. Nitrosation was not linked to the three (NADH-, formate- and glucose-dependent) nitrite reductases but was directly dependent on the presence of a nitrate reductase.
mesh_terms: D004926:Escherichia coli / Q000201:enzymology; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism*; D009565:Nitrate Reductases / Q000378:metabolism*; D009572:Nitrite Reductases / Q000378:metabolism*; D009602:Nitrosamines / Q000096:biosynthesis*; D011511:Proteus / Q000201:enzymology; D011550:Pseudomonas aeruginosa / Q000201:enzymology
chemical_list: D009602:Nitrosamines; D009247:NADH, NADPH Oxidoreductases; D009565:Nitrate Reductases; D009572:Nitrite Reductases