Cloning of cysE gene of Escherichia coli with a mini-Mu-lac containing a plasmid replicon.
The restriction map of cysE gene of Escherichia coli was established after cloning a mini-Mu-lac bacteriophage containing a plasmid replicon and recloning on pBR322 vector plasmid. Enzyme assays of transformants indicated the lack of autoregulation of cysE gene.
D000123:Acetyltransferases / Q000235:genetics / Q000378:metabolism; D001435:Bacteriophages / Q000235:genetics*; D003001:Cloning, Molecular; D003470:Culture Media; D004274:DNA, Recombinant; D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D005822:Genetic Vectors; D010957:Plasmids; D012093:Replicon; D015183:Restriction Mapping; D051050:Serine O-Acetyltransferase; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
D003470:Culture Media; D004274:DNA, Recombinant; D000123:Acetyltransferases; D051050:Serine O-Acetyltransferase; D001616:beta-Galactosidase