Radioelectrophoresis for determination of thyroid hormone binding abnormalities in human serum.
This paper describes a rapid and accurate method for determining binding abilities of thyroid hormones to their corresponding serum proteins: prealbumin, albumin and thyroxine binding globulin. A tube cell agarose gel electrophoresis is used with radioactive labelled triiodothyronine or thyroxine. The distribution curve shows characteristic peaks for prealbumin, albumin and thyroxine binding globulin. A collective of 60 euthyroid thyroid-healthy persons was examined, and the following reference values for the binding of thyroxine to its binding proteins were found: The percentage binding of thyroxine to the prealbumin fraction is 21 to 33%, to the albumin fraction 10 to 15% and to the thyroxine binding globulin fraction 54 to 66%. In a group of 220 patients with suspected thyroid hormone binding abnormalities there were 42 cases of albumin-associated hyperthyroxinaemia, 5 cases of prealbumin-associated hyperthyroxinaemia and 2 cases of thyroxine binding globulin deficiency. In another group of 35 patients with suspected antibodies against thyroid hormones one patient showed antibodies against triiodothyronine.
D001798:Blood Proteins / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D006801:Humans; D011228:Prealbumin / Q000378:metabolism; D011485:Protein Binding; D013963:Thyroid Hormones / Q000097:blood*; D013974:Thyroxine / Q000378:metabolism; D013975:Thyroxine-Binding Proteins / Q000032:analysis; D014284:Triiodothyronine / Q000378:metabolism
D001798:Blood Proteins; D011228:Prealbumin; D013963:Thyroid Hormones; D013975:Thyroxine-Binding Proteins; D014284:Triiodothyronine; D013974:Thyroxine