title: Multilocus genotypes of two strains of Neisseria meningitidis and their presumed variants obtained upon subcultivation.
abstract: Loss of sulfonamide resistance and endotoxin liberation have been described in two strains of Neisseria meningitidis serogroup B, upon subcultivation every 1 to 2 months over an 18-month period. Subsequently, the two laboratory variants, designated 270E- and 840E-, were also found to differ from the parent strains, 270E+ and 840E+, in serotype, outer membrane protein pattern, and virulence in mice. We report here the multilocus genotypes determined by enzyme electrophoresis, of the four isolates 270E+, 270E-, 840E+, and 840E-, and demonstrate that 270E- and 840E- strains could not have originated from subcultivation of 270E+ and 840E+, respectively, but that a mix-up of strains has occurred.
mesh_terms: D000483:Alleles; D005838:Genotype; D005954:Glucosephosphate Dehydrogenase / Q000032:analysis / Q000235:genetics; D006801:Humans; D007521:Isocitrate Dehydrogenase / Q000032:analysis / Q000235:genetics; D008291:Malate Dehydrogenase / Q000032:analysis / Q000235:genetics; D009345:Neisseria meningitidis / Q000145:classification / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D010447:Peptide Hydrolases / Q000032:analysis / Q000235:genetics; D012703:Serotyping
chemical_list: D008291:Malate Dehydrogenase; D007521:Isocitrate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D010447:Peptide Hydrolases