Identification and subcellular location of talin in various cell types and tissues by means of [125I]vinculin overlay, immunoblotting and immunocytochemistry.
In the present study, we have examined the cellular and subcellular distribution of talin in several tissues of the chicken. By immunocytochemistry, Western Blot analysis and [125I]vinculin overlay, talin was demonstrated in most of the main tissues and cell types of the body. Corresponding to the property of talin to bind to the fibronectin receptor, talin was found to be confined to the site of the plasma membrane that abuts the extracellular matrix in various types of mesenchymal and epithelial cells. In the central nervous system talin was almost exclusively confined to cells of the connective tissue, i.e., blood vessels and the connective tissue sheaths. No evidence was obtained for the association of talin with any type of intercellular junction. In nonadhering cells such as circulating platelets and leukocytes, talin displayed a diffuse distribution throughout the cytoplasm. These findings suggest a general role for talin in certain aspects of cellular adhesion to the extracellular matrix.
D000818:Animals; D001773:Blood Cells / Q000032:analysis* / Q000648:ultrastructure; D002645:Chickens; D003238:Connective Tissue / Q000032:analysis* / Q000648:ultrastructure; D003598:Cytoskeletal Proteins / Q000032:analysis*; D007150:Immunohistochemistry; D007158:Immunologic Techniques; D008854:Microscopy, Electron; D009124:Muscle Proteins; D009132:Muscles / Q000032:analysis* / Q000648:ultrastructure; D016608:Talin; D016596:Vinculin
D003598:Cytoskeletal Proteins; D009124:Muscle Proteins; D016608:Talin; D016596:Vinculin