Identification of 6-keto-prostaglandin E1 obtained from isolated perfused kidney of the rabbit.
We studied the release of 6-keto-prostaglandin (PG) E1-like material into urinary and venous effluents of the isolated perfused kidney of the rabbit. After high performance liquid chromatographic (HPLC) separation, material coeluting with authentic 6-keto-PGE1 was measured by radioimmunoassay, platelet antiaggregation bioassay and characterized further by gas chromatography/mass spectrometry-single ion monitoring (GC/MS-SIM). Injection of either arachidonic acid or ATP into the renal artery stimulated the release of prostacyclin [measured as immunoreactive (i)-6-keto-PGF1 alpha] and i-6-keto-PGF1. HPLC fractions containing i-6-keto-PGE1, and coeluting with 6-keto-PGE1 standard, exhibited potent inhibition of platelet aggregation. The presence of authentic 6-keto-PGE1 was verified by the GC/MS-SIM spectra in HPLC zones from which radioimmunoassayable and bioassayable 6-keto-PGE1-like material was recovered.
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000527:Alprostadil / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000276:immunology / Q000302:isolation & purification; D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000494:pharmacology; D011464:Epoprostenol / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D066298:In Vitro Techniques; D007668:Kidney / Q000378:metabolism*; D010477:Perfusion; D010974:Platelet Aggregation; D011817:Rabbits
D001095:Arachidonic Acids; D016718:Arachidonic Acid; C022950:6-ketoprostaglandin E1; D000255:Adenosine Triphosphate; D011464:Epoprostenol; D000527:Alprostadil