Mechanism of suppression of lipopolysaccharide-driven B cell differentiation by anti-mu antibodies. Evidence for a trans-acting repressor of transcription.
Bivalent anti-mu antibodies suppress LPS-driven B cell differentiation by inhibiting the coordinate activation of a family of differentiation-related genes, including those encoding the heavy, light, and J chains of IgM. We have shown that the presence of inhibitors of RNA or protein synthesis during a pulse with anti-mu can interfere with induction of suppression. We suggest that suppression is mediated by a trans-acting repressor protein with specificity for common motifs in regulatory regions of each of these genes.
D000546:Amanitins / Q000494:pharmacology; D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D001402:B-Lymphocytes / Q000166:cytology*; D002454:Cell Differentiation; D003839:Deoxyadenosines / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004640:Emetine / Q000494:pharmacology; D005260:Female; D007148:Immunoglobulin mu-Chains / Q000276:immunology*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D051379:Mice; D008810:Mice, Inbred C57BL; D012333:RNA, Messenger / Q000032:analysis; D012097:Repressor Proteins / Q000494:pharmacology*; D014157:Transcription Factors / Q000494:pharmacology*; D014158:Transcription, Genetic
D000546:Amanitins; D000888:Antibodies, Anti-Idiotypic; D003839:Deoxyadenosines; D007148:Immunoglobulin mu-Chains; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D012097:Repressor Proteins; D014157:Transcription Factors; D004640:Emetine