Localization of specific carbohydrate configurations in the hemophilic synovial membrane.
Fluorescein isothiocyanate-conjugated lectins include: concanavalin A (Con-A), Dolichos biflorus agglutinin (DBA), Griffonia simplicifolia-I (GS-I), Griffonia simplicifolia-II (GS-II), Arachis hypogaea agglutinin (PNA), Maclura pomifera agglutinin (MPA), Ricinus communis agglutinin-I (RCA-I), Glycine max agglutinin (SBA), Ulex europaeus agglutinin-I (UEA-I), and wheat germ agglutinin (WGA). These lectins were used to histochemically demonstrate lectin bindings on hemophilic synovial membrane. GS-I (galactose and galactosamine specific) and SBA (galactosamine specific) was shown to bind strongly in the cytoplasm of the lining cells. Lymphocytes and mast cells were largely bound by Con-A (mannose and glucose specific) and GS-II (glucosamine specific) positive. UEA-1 (fucose specific) was shown to bind specifically to synovial vascular endothelial cells. Lectin histochemistry is a useful method for classification of the cells on the synovial membrane.
D050260:Carbohydrate Metabolism; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D006467:Hemophilia A / Q000378:metabolism*; D006801:Humans; D037102:Lectins; D013583:Synovial Membrane / Q000378:metabolism*; D013861:Thiocyanates
D005452:Fluoresceins; D037102:Lectins; D013861:Thiocyanates; D016650:Fluorescein-5-isothiocyanate