Human platelet interactions with surfaces of type I collagen, chondroitin-4-sulphate, and chondroitin-6-sulphate in vitro.
In vitro human platelet interactions with surfaces of type I collagen, chondroitin-6-sulphate (CH-6-S), chondroitin-4-sulphate (CH-4-S), a CH-6-S/collagen layer, and a collagen-CH-6-S complex were investigated. Polystyrene and silanized glass served as controls. Platelet counts, platelet factor 4 released, and platelet aggregating ability for the different surfaces were compared with controls. Platelet count and platelet factor 4 release data showed that there were no differences between surfaces of CH-6-S, CH-4-S and the controls. However, significant differences in platelet counts and platelet factor 4 released were found when collagen, the CH-6-S/collagen layer, and the collagen-CH-6-S complex were compared with controls. The pure type I collagen surface had the greatest influence on platelet activation. The collagen-CH-6-S complex had a greater effect that the CH-6-S/collagen layer on platelet activation. It appears that chondroitin-6-sulphate can modify the platelet activity of type I collagen.
D001792:Blood Platelets / Q000187:drug effects / Q000502:physiology*; D002807:Chondroitin / Q000031:analogs & derivatives*; D002809:Chondroitin Sulfates / Q000494:pharmacology*; D003094:Collagen / Q000494:pharmacology*; D005898:Glass; D006801:Humans; D010974:Platelet Aggregation / Q000187:drug effects; D010976:Platelet Count; D010978:Platelet Factor 4 / Q000502:physiology; D011137:Polystyrenes
D011137:Polystyrenes; D010978:Platelet Factor 4; D002807:Chondroitin; D002809:Chondroitin Sulfates; D003094:Collagen