Biosynthetic origin of aflatoxin G1: confirmation of sterigmatocystin and lack of confirmation of aflatoxin B1 as precursors.
The origin of aflatoxin G1 was studied using mutant strains of Aspergillus parasiticus blocked early in the pathway and by tracing 14C-labelled aflatoxin B1 (AFB1) in wild-type A. flavus and A. parasiticus strains. Sterigmatocystin (ST) was a precursor of AFB1, AFG1 and AFG2 in the four mutants examined. The identity of AFG1 was confirmed by mass spectrometry. No evidence for conversion of AFB1 to AFG1 was found. A rigorously controlled study of conversions of radioactivity based on preparative thin-layer chromatography of aflatoxins demonstrated that low levels of aflatoxin interconversions previously reported in the literature might actually be artifacts.
D016604:Aflatoxin B1; D000348:Aflatoxins / Q000096:biosynthesis* / Q000378:metabolism*; D001230:Aspergillus / Q000378:metabolism; D001711:Biotransformation; D002855:Chromatography, Thin Layer; D013241:Sterigmatocystin / Q000493:pharmacokinetics*; D014966:Xanthenes / Q000493:pharmacokinetics*
D000348:Aflatoxins; D014966:Xanthenes; D013241:Sterigmatocystin; C027955:aflatoxin G1; C029754:aflatoxin G2; C029753:aflatoxin B2; D016604:Aflatoxin B1