Acid pH enhances the effects of taurodeoxycholate on water and solute transport in the human and rat jejunum.
The relative rate of unspecific binding of bile acids to brush border membrane vesicles resembles their relative potencies as intestinal secretagogues. This interaction of bile acids with brush border membranes is enhanced in an acid environment. We, therefore, studied the effects of taurodeoxycholate and taurocholate on water and solute transport at pH 7.6 and pH 4.0 in the human and rat jejunum. Five mM taurodeoxycholate induced significantly greater fluid secretion in the human jejunum at pH 4.0 than at pH 7.6 (p less than 0.02; n = 5), 10 mM taurocholate (n = 4) had no effect at either pH. In the rat 15 mM taurodeoxycholate at pH 4.0 induced greater fluid secretion (p less than 0.01; n = 6), released more phospholipid (p less than 0.001; n = 4) and enhanced absorption of mannitol more than at pH 7.6 (p less than 0.05; n = 6). In contrast fluid secretion and release of phospholipids induced by Triton X-100 were not affected by pH (n = 6), nor was fluid secretion induced by cholera toxin (n = 8). The data suggest that the enhancement of the secretory effect of taurodeoxycholate in an acid environment is due to its increased interaction with the mucosal surface, and support the concept that the ability of detergents to interact with the intestinal brush border membrane determines their effectiveness as intestinal secretagogues.
D000818:Animals; D001647:Bile Acids and Salts / Q000378:metabolism; D001834:Body Water / Q000378:metabolism*; D002772:Cholera Toxin / Q000494:pharmacology; D003840:Deoxycholic Acid / Q000031:analogs & derivatives*; D004573:Electrolytes / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007408:Intestinal Absorption / Q000187:drug effects; D007419:Intestinal Secretions / Q000187:drug effects / Q000378:metabolism*; D007583:Jejunum / Q000378:metabolism; D008297:Male; D008353:Mannitol / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013657:Taurodeoxycholic Acid / Q000494:pharmacology*
D001647:Bile Acids and Salts; D004573:Electrolytes; D010743:Phospholipids; D003840:Deoxycholic Acid; D008353:Mannitol; D013657:Taurodeoxycholic Acid; D002772:Cholera Toxin; D005947:Glucose