title: Characterization of the bacteriophage B2 of Lactobacillus plantarum ATCC 8014.
abstract: Bacteriophage B2 of Lactobacillus plantarum ATCC 8014, isolated in 1971, belonged to Bradley's group B. Electron microscopy revealed an isometric head (110 nm) and a long non-contractile and flexible tail (500 nm) containing about 75 regularly aligned lateral striations. Burst size was 12-14 phages per infectious centre. The latent period for phage development was 75 min and the rise period approximately 90 min. The phage particle contained 5 major proteins. The buoyant density of the phage in CsCl was measured as 1.575 g/cm3. B2 genome was a linear double-stranded DNA molecule of 37 +/- 1% guanosine-cytosine. Its size was 73 kilobase pairs (kbp). Restriction analysis of the genome showed that 4 restriction enzymes (Xba I, Sac I, Bgl II and Sma I) gave single site cuts in the DNA, while Ava I and Sal I formed 2 and 5 cuts, respectively.
mesh_terms: D001435:Bacteriophages / Q000235:genetics / Q000502:physiology* / Q000648:ultrastructure; D004247:DNA / Q000032:analysis; D004279:DNA, Viral / Q000032:analysis; D005796:Genes; D007778:Lactobacillus / Q000502:physiology*; D008854:Microscopy, Electron
chemical_list: D004279:DNA, Viral; D004247:DNA