Alterations of granulopoiesis in chronic uremic patients treated with intermittent hemodialysis.
Granulocyte-macrophage progenitor cells (CFU-GM), leukocyte colony-stimulating activity (CSA), granuloblast differentiation and proliferation and the effect of uremic serum on the in vitro growth of normal CFU-GM have been studied in 8 chronic uremic patients treated with intermittent hemodialysis three times a week. The studies were performed in the postabsorptive state twice in each patient, that is at the longest and shortest dialytic interval. CFU-GM growth in agar and leukocyte CSA did not differ significantly from the normal level in uremic subjects. The granulocytic and macrophagic differentiation in a liquid culture system was significantly reduced in uremic patients, notwithstanding the appearance of high numbers of undifferentiated blastic cells. The serum of uremic patients had no effect on normal CFU-GM and leukocyte CSA.
D000328:Adult; D000368:Aged; D002454:Cell Differentiation; D002455:Cell Division; D002908:Chronic Disease; D003115:Colony-Stimulating Factors / Q000378:metabolism; D005260:Female; D006098:Granulocytes / Q000473:pathology / Q000502:physiology*; D006410:Hematopoiesis; D006801:Humans; D007962:Leukocytes / Q000378:metabolism; D008264:Macrophages / Q000473:pathology / Q000502:physiology; D008297:Male; D008875:Middle Aged; D006435:Renal Dialysis; D013234:Stem Cells / Q000473:pathology; D014511:Uremia / Q000097:blood / Q000473:pathology / Q000628:therapy*
D003115:Colony-Stimulating Factors