Transformation of Penicillium chrysogenum using the Aspergillus nidulans amdS gene as a dominant selective marker.
The Aspergillus nidulans acetamidase gene (amdS) has been used to transform Penicillium chrysogenum at low frequency. Several transformants were tested and shown to be mitotically stable. Southern blot analysis indicated that transforming DNA had integrated into the chromosomal DNA, possibly at multiple sites.
D001233:Aspergillus nidulans / Q000235:genetics*; D005799:Genes, Dominant; D005800:Genes, Fungal; D009693:Nucleic Acid Hybridization; D010407:Penicillium / Q000235:genetics*; D010408:Penicillium chrysogenum / Q000235:genetics*; D014170:Transformation, Genetic