Identification of an alternatively spliced Kd and the Qa-6d mRNAs by using amplified cDNA.
We have employed the primer chain reaction method for direct sequencing of H-2 mRNAs. This approach is highly sensitive and permits quantitation and sequencing of the canonical as well as alternatively spliced mRNAs that may be expressed at 5-10% level in comparison to the major H-2 species. Using this technique, we have identified a novel species of alternatively spliced Kd mRNA expressed in L1210 lymphoma and in the spleen and liver of DBA/2 mice. Similarly, we found a previously described alternatively spliced species of H-2Dd mRNA to be expressed in L1210 lymphoma and have determined the sequence of the cytoplasmic domain of Ld mRNA. In addition, we have identified a Class I MHC transcript presumably encoded by a gene allelic to Q6 gene of BALB/c mice.
D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000096:biosynthesis; D005091:Exons; D005784:Gene Amplification; D005802:Genes, MHC Class II; D006183:H-2 Antigens / Q000276:immunology*; D007939:Leukemia L1210 / Q000276:immunology; D008099:Liver / Q000032:analysis; D051379:Mice; D008811:Mice, Inbred DBA; D008969:Molecular Sequence Data; D012326:RNA Splicing; D012333:RNA, Messenger / Q000032:analysis*; D013154:Spleen / Q000032:analysis
D006183:H-2 Antigens; D012333:RNA, Messenger; D004247:DNA