Serological and biochemical characterization of surface antigen expressed on human myeloid cells and identification of six distinct antigen systems.
Six distinct surface antigens selectively expressed on human myeloid cells were defined with ten murine monoclonal antibodies. Four antigens (GAp95, GAp170, GAp160-220, GAp145-200) are expressed on protein molecules and one antigen (GANGL) is detected in neutral glycolipids. The biochemical nature of the remaining one (GA8.9) is unknown. GAp95 and GANGL are expressed on relatively mature myeloid cells, whereas GAp160-220 is expressed on immature myeloid cells. GAp170 and GAp145-200 are expressed on a wide range of myeloid cells. GA8.9 was found on NKM-1, a cell line of acute myelomonocytic leukemia, and also on some acute nonlymphocytic leukemia cells.
D000911:Antibodies, Monoclonal / Q000276:immunology; D000951:Antigens, Neoplasm / Q000302:isolation & purification*; D000954:Antigens, Surface / Q000302:isolation & purification*; D005455:Fluorescent Antibody Technique; D006017:Glycolipids / Q000276:immunology; D006382:Hemadsorption; D006412:Hematopoietic Stem Cells / Q000276:immunology; D006801:Humans; D007104:Immune Adherence Reaction; D007124:Immunoenzyme Techniques; D007951:Leukemia, Myeloid / Q000276:immunology; D014407:Tumor Cells, Cultured / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D006017:Glycolipids