Synthesis of nuclear lamins in BHK-21 cells synchronized with aphidicolin.
Lamins A, B and C are the major proteins of mammalian nuclear lamina and have been well studied in BHK-21 cells. By synchronizing BHK-21 cells with aphidicolin, a potent inhibitor of DNA alpha-polymerase, we were able to detect a differential pattern of synthesis for nuclear lamins during the cell cycle. Lamin B starts to be synthesized only in S phase up to mitosis while synthesis of lamins A and C remain stable throughout the cell cycle. The precursor of lamin A see its half-life increase from a reported 63 min in interphase cells to 103 min in G2/M cells.
D000818:Animals; D016590:Aphidicolin; D002453:Cell Cycle / Q000187:drug effects; D002460:Cell Line; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism*; D004261:DNA Replication / Q000187:drug effects; D004224:Diterpenes / Q000494:pharmacology*; D007700:Kinetics; D034904:Lamin Type A; D034921:Lamin Type B; D034882:Lamins; D008715:Methionine / Q000378:metabolism; D009687:Nuclear Proteins / Q000096:biosynthesis*; D014176:Protein Biosynthesis; D013936:Thymidine / Q000378:metabolism
D004224:Diterpenes; D034904:Lamin Type A; D034921:Lamin Type B; D034882:Lamins; D009687:Nuclear Proteins; D016590:Aphidicolin; D008715:Methionine; D013936:Thymidine