Inducibility of rat brain drug-metabolizing enzymes.
Cytochrome P450 from rat brain mitochondrial and microsomal fractions was found to be inducible by 3-methylcholanthrene, both in quantity of enzyme and in activity towards 7-ethoxyresorufin, which is a model substrate for the cytochrome P450 isoform specifically induced by 3-methylcholanthrene. Conversely, a phenobarbital treatment resulted in an induction of the microsomal cytochrome P450 only. On the other hand, the microsomal 1-naphthol-UDP-glucuronosyl transferase and epoxide hydrolase seemed to be non-inducible by 3-methylcholanthrene or by phenobarbital. The toxicological implications of these data are discussed.
D000818:Animals; D001921:Brain / Q000201:enzymology*; D001923:Brain Chemistry; D019363:Cytochrome P-450 CYP1A1; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis; D004790:Enzyme Induction; D004851:Epoxide Hydrolases / Q000096:biosynthesis; D014453:Glucuronosyltransferase / Q000096:biosynthesis; D008099:Liver / Q000032:analysis / Q000201:enzymology; D008297:Male; D008748:Methylcholanthrene / Q000494:pharmacology; D010088:Oxidoreductases / Q000096:biosynthesis; D010599:Pharmacokinetics; D010634:Phenobarbital / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains
D008748:Methylcholanthrene; D003577:Cytochrome P-450 Enzyme System; D010088:Oxidoreductases; D019363:Cytochrome P-450 CYP1A1; D014453:Glucuronosyltransferase; D004851:Epoxide Hydrolases; D010634:Phenobarbital