title: Early prenatal diagnosis of congenital toxoplasmosis using amniotic fluid samples and tissue culture.
abstract: The presence of Toxoplasma gondii in amniotic fluid was demonstrated using tissue culture in four of nine cases of congenital toxoplasmosis, whereas Toxoplasma gondii antigen was undetectable using a sensitive enzyme immunoassay technique. Parasites were identified in monolayers four days after inoculation using an indirect immunofluorescence assay. Since tissue culture may provide evidence of infection within a few days, this method is proposed for early prenatal diagnosis of congenital toxoplasmosis.
mesh_terms: D000653:Amniotic Fluid / Q000469:parasitology*; D000818:Animals; D000953:Antigens, Protozoan / Q000032:analysis; D046508:Culture Techniques; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D011247:Pregnancy; D011296:Prenatal Diagnosis; D014122:Toxoplasma / Q000276:immunology / Q000302:isolation & purification*; D014125:Toxoplasmosis, Congenital / Q000175:diagnosis* / Q000276:immunology
chemical_list: D000953:Antigens, Protozoan