title: Juvenile hormone action mediated in male accessory glands of Drosophila by calcium and kinase C.
abstract: In an in vitro system for the Drosophila melanogaster male accessory gland, it was found that 10(-9)M juvenile hormone III could accurately mimic the copulation-induced response of increased protein synthesis in glands from virgin flies. Stimulation by this hormone required calcium in the medium. Experiments with tumor-promoting phorbol esters indicated that activation of protein kinase C can also cause the glands to increase protein synthesis. Stimulation of protein synthesis by juvenile hormone did not occur in mutants deficient in kinase C activity. These results suggest a membrane-protein-mediated effect of juvenile hormone that involves calcium and kinase C.
mesh_terms: D000818:Animals; D002118:Calcium / Q000502:physiology*; D004331:Drosophila melanogaster / Q000378:metabolism*; D004789:Enzyme Activation / Q000187:drug effects; D005837:Genitalia, Male / Q000187:drug effects / Q000378:metabolism; D007605:Juvenile Hormones / Q000235:genetics; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism; D009154:Mutation; D015240:Phorbol 12,13-Dibutyrate; D010703:Phorbol Esters / Q000494:pharmacology; D014176:Protein Biosynthesis; D011493:Protein Kinase C / Q000378:metabolism*; D012717:Sesquiterpenes / Q000494:pharmacology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D007605:Juvenile Hormones; D008565:Membrane Proteins; D010703:Phorbol Esters; D012717:Sesquiterpenes; D015240:Phorbol 12,13-Dibutyrate; C036585:juvenile hormone III; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium