title: Molecular cloning of bovine class I MHC cDNA.
abstract: Two cDNA cloned from a Hereford cow B cell line (BL-3) have allowed the determination of the complete coding region for two class I molecules encoded by the bovine MHC (BoLA). The predicted protein sequences have all the features expected of expressed class I molecules that present peptide Ag to cytotoxic T cells. Comparison with class I molecules from other species strongly suggests these cDNA are derived from different genes and provides evidence for the existence of a second expressed class I BoLA locus. The BoLA proteins show greater similarity to HLA than to H-2 molecules, correlating with the cross-reactions of W6/32 and other murine anti-HLA-A,B,C mAb with BoLA molecules. The basis for the W6/32 epitope and the preferential association of H-2 class I H chains with bovine beta 2-m is examined.
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002462:Cell Membrane / Q000378:metabolism; D003001:Cloning, Molecular; D003429:Cross Reactions; D004247:DNA / Q000302:isolation & purification*; D005260:Female; D005805:Genes, MHC Class I; D006680:HLA Antigens / Q000276:immunology; D006649:Histocompatibility Antigens / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D021382:Protein Sorting Signals / Q000235:genetics / Q000302:isolation & purification; D012689:Sequence Homology, Nucleic Acid; D001613:beta 2-Microglobulin / Q000235:genetics
chemical_list: D006680:HLA Antigens; D006649:Histocompatibility Antigens; D007143:Immunoglobulin Heavy Chains; D021382:Protein Sorting Signals; D001613:beta 2-Microglobulin; D004247:DNA