title: Cell-free translation of mRNA encoding an arterial smooth muscle cell proteoglycan core protein.
abstract: The size and immunological reactivity of the primary gene products of a small non-aggregating dermatan sulfate proteoglycan from bovine and monkey arterial smooth muscle cells were examined after cell-free translation of mRNA. Antisera against the dermatan sulfate proteoglycans from bovine articular cartilage, DSPG II [Rosenberg et al. J. Biol. Chem. 260, 6304 (1985)] and human skin fibroblasts [Glossl et al. J. Biol. Chem. 259, 14144 (1984)] were used to show that the unmodified smooth muscle precursor core protein was immunologically related to both the cartilage and fibroblast core proteins. The size of the precursor core proteins within each species was identical regardless of the tissue source. Comparison of the precursor core proteins synthesized by primate and bovine cells revealed that the bovine core proteins were approximately 1500 Da larger than the primate core proteins as determined by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis. A similar size difference was observed when the mature core proteins of monkey smooth muscle cells and bovine articular chondrocytes were compared after removal of the glycosaminoglycan chains. These results indicate that arterial smooth muscle cells synthesize a dermatan sulfate proteoglycan whose core protein is similar to, if not the same as, the cartilage and fibroblast dermatan sulfate proteoglycan core proteins. These core proteins may be encoded by the same gene that has diverged in size during speciation.
mesh_terms: D053674:Aggrecans; D000818:Animals; D002356:Cartilage / Q000032:analysis / Q000166:cytology; D002417:Cattle; D016326:Extracellular Matrix Proteins; D006023:Glycoproteins / Q000032:analysis / Q000235:genetics*; D000882:Haplorhini; D037181:Lectins, C-Type; D009131:Muscle, Smooth, Vascular / Q000032:analysis / Q000166:cytology*; D014176:Protein Biosynthesis; D011509:Proteoglycans; D012333:RNA, Messenger / Q000235:genetics*
chemical_list: D053674:Aggrecans; D016326:Extracellular Matrix Proteins; D006023:Glycoproteins; D037181:Lectins, C-Type; D011509:Proteoglycans; D012333:RNA, Messenger