Restriction of anti-peptide antibody specificity by enzyme-modified Sepharose-peptide immunoadsorbents.
Sepharose-peptide immunoadsorbents, employed for the isolation of specific antibodies from the sera of rabbits immunized with carrier protein-peptide conjugates, were digested with suitable proteolytic enzymes, in order to obtain the splitting of a part of the peptide bound to the gel. This new modified immunoadsorbent can be advantageously used for the isolation of antibody subsets, that do not cross-react with related peptides exhibiting high sequence homology with the immunogens.
D000595:Amino Acid Sequence; D000818:Animals; D000918:Antibody Specificity; D007163:Immunosorbent Techniques; D008969:Molecular Sequence Data; D010455:Peptides / Q000276:immunology*; D011817:Rabbits; D012685:Sepharose
D010455:Peptides; D012685:Sepharose