title: Enzyme immunoassay for detection of Drosophila melanogaster antigens in the juice of various foods.
abstract: An enzyme immunoassay (EIA) was developed to identify and quantitate soluble antigens originating from Drosophila melanogaster eggs. Polystyrene microtiter plates were coated with anti-egg antibody. Egg antigen standards or samples were reacted with the sensitized wells. The immobilized antigen was reacted with biotinylated antibody, and the complex was quantitated by an avidin-horseradish peroxidase conjugate. The assay can detect egg antigens equivalent to 0.03 eggs/mL. The antibody was directed against a number of antigens and cross-reacted with extracts of adult Drosophila melanogaster flies. Homogenized eggs diluted in various juice samples were detectable by EIA. Heating the antigen diluted in buffer or juice at a temperature used in processing of juice reduced the immunological response approximately 40-45%. Brine separated from fresh-packed sauerkraut showed EIA responses equivalent to 0.3-3 eggs/mL. Electrophoretic, chromatographic, and immunological analyses suggested that the EIA responses for sauerkraut brine were related to the Drosophila melanogaster egg antigens.
mesh_terms: D000818:Animals; D000941:Antigens / Q000032:analysis*; D001628:Beverages / Q000032:analysis*; D001710:Biotin / Q000032:analysis; D002850:Chromatography, Gel; D002957:Citrus; D004331:Drosophila melanogaster / Q000276:immunology*; D005504:Food Analysis; D005638:Fruit; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000032:analysis; D013056:Spectrophotometry, Ultraviolet; D013205:Staphylococcal Protein A / Q000032:analysis; D014675:Vegetables
chemical_list: D000941:Antigens; D007074:Immunoglobulin G; D013205:Staphylococcal Protein A; D001710:Biotin