title: Mechanisms of reduction of tumor recurrence with carbon dioxide laser in experimental mammary tumors.
abstract: This study compares local tumor recurrence after low energy CO2 laser wound sterilization with recurrence after scalpel, laser or electrocautery excision. Wound histologic changes were studied to understand the mechanism of the interaction between the laser and wound. Single implants of R3230AC mammary tumor were grown to an average diameter of 24 millimeters in the mammary ridge of 80 female fisher 344 rats. Rats were anesthesized with pentobarbital and randomized into groups, each with similar tumor size: scalpel (S), laser (L), laser with wound sterilization (LV), scalpel with sterilization (SV) and electrocautery (E). All surgical procedures were performed by the same surgeon with the same technique, with the exception of the instruments used. Tow rats from each group were sacrificed immediately and the wounds examined histologically. The Sharplan 1100 CO2 laser was used with a 125 millimeter hand piece in focus and in continuous wave for groups L and LV. Sterilization in groups LV and SV was performed with 5 millimeter spot size by heating the site gently without causing blanching of tissue. Excision in group E was performed with coagulating current from a monopolar cautery (Valley Lab). Rats were examined periodically for 30 days and those dying during this period were excluded from analysis. The incidence of wound recurrence was eight of 12 in group S; five of eight, L; four of 13, E; three of 12, LV, and two of nine, SV (p less than 0 .05). Histologic changes in the wound demonstrated viable tumor in all groups, with fewer areas present in groups E, SV and LV. Local thermal effects and the noncontact nature of the CO2 laser make it an effective adjunct in reducing local tumor recurrence by enhancing the cytoreductive capability of surgical procedures.
mesh_terms: D000230:Adenocarcinoma / Q000473:pathology / Q000601:surgery*; D000818:Animals; D002245:Carbon Dioxide; D003131:Combined Modality Therapy; D004564:Electrocoagulation; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D053685:Laser Therapy; D008325:Mammary Neoplasms, Experimental / Q000473:pathology / Q000601:surgery*; D009364:Neoplasm Recurrence, Local / Q000473:pathology / Q000517:prevention & control*; D011897:Random Allocation; D051381:Rats; D011916:Rats, Inbred F344; D013242:Sterilization; D013525:Surgical Instruments
chemical_list: D002245:Carbon Dioxide