Elongating ribosomes in vivo are refractory to erythromycin.
We have studied the kinetics of erythromycin inhibition of translation in growing bacteria. In order to simplify the interpretation of our data, we have used a mutant (envA), known to have an increased permeability to several antibiotics, including erythromycin. The data clearly show that an initial stage of translation is sensitive to erythromycin, but that the elongating ribosome is insensitive to the antibiotic.
D004790:Enzyme Induction / Q000187:drug effects; D004917:Erythromycin / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D010441:Peptide Chain Elongation, Translational / Q000187:drug effects; D014176:Protein Biosynthesis; D011691:Puromycin / Q000494:pharmacology; D012270:Ribosomes / Q000187:drug effects*; D001616:beta-Galactosidase / Q000096:biosynthesis
D011691:Puromycin; D004917:Erythromycin; D001616:beta-Galactosidase