Prebeta-migrating high density lipoprotein: quantitation in normal and hyperlipidemic plasma by solid phase radioimmunoassay following electrophoretic transfer.
A quantitative solid phase immunoassay has been developed for the determination of the mass of electrophoretically separated prebeta apolipoprotein A-I (apoA-I) in human plasma. Conditions have been identified for the quantitative transfer and immunoblotting of the apolipoprotein in the absence of organic solvents or detergents. In normolipidemic plasma, the prebeta-migrating fraction of apoA-I represented 4.2 +/- 1.8% of total apoA-I (61 +/- 26 micrograms of apoA-I per ml of plasma). Significantly higher levels were found in hypercholesterolemia of genetic origin, in primary and secondary hypertriglyceridemia, and in congenital lecithin:cholesterol acyltransferase deficiency. In all cases prebeta-migrating apoA-I consisted in large part of low molecular weight lipoprotein species, compared to the size of the major, alpha-migrating apoA-I fraction.
D016632:Apolipoprotein A-I; D001054:Apolipoproteins A / Q000097:blood*; D001797:Blood Protein Electrophoresis; D006801:Humans; D006949:Hyperlipidemias / Q000097:blood*; D007123:Immunoelectrophoresis, Two-Dimensional; D007074:Immunoglobulin G / Q000032:analysis; D007457:Iodine Radioisotopes; D008075:Lipoproteins, HDL / Q000097:blood*; D008970:Molecular Weight; D011863:Radioimmunoassay
D016632:Apolipoprotein A-I; D001054:Apolipoproteins A; D007074:Immunoglobulin G; D007457:Iodine Radioisotopes; D008075:Lipoproteins, HDL