title: Immunoelectron microscopic localization of small, acid-soluble spore proteins in sporulating cells of Bacillus subtilis.
abstract: Small, acid-soluble spore proteins SASP-alpha, SASP-beta, and SASP-gamma as well as a SASP-beta-lacZ gene fusion product were found only within the forespore compartment of sporulating Bacillus subtilis cells by using immunoelectron microscopy. The alpha/beta-type SASP were associated almost exclusively with the forespore nucleoid, while SASP-gamma was somewhat excluded from the nucleoid. These different locations of alpha/beta-type and gamma-type small, acid-soluble spore proteins within the forespore are consistent with the different roles for these two types of proteins in spore resistance to UV light.
mesh_terms: D001412:Bacillus subtilis / Q000502:physiology / Q000648:ultrastructure*; D001426:Bacterial Proteins / Q000032:analysis*; D007124:Immunoenzyme Techniques; D008854:Microscopy, Electron; D012808:Sigma Factor; D013171:Spores, Bacterial / Q000648:ultrastructure; D014157:Transcription Factors
chemical_list: D001426:Bacterial Proteins; D012808:Sigma Factor; D014157:Transcription Factors; C092468:spoIIR protein, Bacillus subtilis; C030069:spore-specific proteins, Bacillus