title: Defective DNA excision repair in cells of patients with homocystinuria.
abstract: Fibroblasts obtained from biopsied material and lymphocytes from patients with homocystinuria were studied for repair activity using the criterion of repair of DNA breaks induced by 4-nitroquinoline 1-oxide and gamma-irradiation and criteria of reactivation and induced mutagenesis of vaccinia virus. Lymphocytes showed defective DNA repair for all these criteria. In fibroblast cultures, the inhibition of cell-repair activity for the gamma-type was retained. The number of spontaneous and gamma-induced virus mutations increased as passaging of fibroblasts proceeded.
mesh_terms: D015112:4-Nitroquinoline-1-oxide; D002478:Cells, Cultured; D004249:DNA Damage / Q000528:radiation effects; D004260:DNA Repair; D005347:Fibroblasts; D005720:Gamma Rays; D006367:HeLa Cells; D006712:Homocystinuria / Q000235:genetics*; D006801:Humans; D008214:Lymphocytes / Q000502:physiology; D014616:Vaccinia virus / Q000235:genetics; D014779:Virus Replication; D014983:Xeroderma Pigmentosum / Q000235:genetics
chemical_list: D015112:4-Nitroquinoline-1-oxide