Simple graphical methods for use with complex ligand-binding and enzyme mechanisms.
Simple graphical plots in distribution-free space are shown to provide estimates of mechanism and kinetic parameters for complex steady-state processes. The methods were tested using simulated 1:1, 1:2, 2:2, 2:3 and 3:3 functions, and real data from polyol dehydrogenase, NADPH oxidation in the presence of DL-glyceraldehyde, and NADPH oxidation in the presence of an NADPH-binding protein. Estimates of mechanism and parameters agreed closely with those found from non-linear regression by computer.
D000449:Aldehyde Reductase / Q000494:pharmacology; D000818:Animals; D002021:Buffers; D002417:Cattle; D005985:Glyceraldehyde / Q000494:pharmacology; D007700:Kinetics; D007908:Lens, Crystalline / Q000201:enzymology; D008433:Mathematics; D009249:NADP / Q000378:metabolism*; D010084:Oxidation-Reduction; D010710:Phosphates / Q000494:pharmacology; D011188:Potassium / Q000494:pharmacology; D017680:Potassium Compounds; D013401:Sugar Alcohol Dehydrogenases / Q000378:metabolism*
D002021:Buffers; D010710:Phosphates; D017680:Potassium Compounds; D005985:Glyceraldehyde; D009249:NADP; C013216:potassium phosphate; D013401:Sugar Alcohol Dehydrogenases; D000449:Aldehyde Reductase; C023142:polyol dehydrogenase (NADP); D011188:Potassium