title: Human liver type pyruvate kinase: complete amino acid sequence and the expression in mammalian cells.
abstract: Pyruvate kinase (PK) has four isozymes (L, R, M1, M2) that are encoded by two different genes. Among these isozymes, abnormalities of liver (L)-type PK is considered to be associated with hereditary nonspherocytic hemolytic anemia in humans. We isolated and determined the full-length sequence of human L-type PK cDNA. The cDNA contains 1629 base pairs encoding 543 amino acids, 68 base pairs of 5'-noncoding sequence, and 734 base pairs of 3'-noncoding sequence. The similarity between human and rat L-type PK was 86.9% at the nucleotide sequence level and 92.4% at the amino acid sequence level. The full-length L-type PK cDNA was placed under the promoter of simian virus 40 and introduced into monkey COS cells. Human L-type PK activity was detected in the extract of COS cells by the classical PK electrophoresis method.
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000032:analysis; D000882:Haplorhini; D006801:Humans; D008099:Liver / Q000201:enzymology*; D008297:Male; D008969:Molecular Sequence Data; D011770:Pyruvate Kinase / Q000032:analysis*; D014162:Transfection
chemical_list: D004247:DNA; D011770:Pyruvate Kinase