5S RNA-protein complexes released by EDTA treatment of 60S ribosomal subunits of Tetrahymena thermophila.
Treatment of large (60S) subunit of the cytoplasmic ribosome of the protozoa Tetrahymena thermophila with EDTA causes quantitative release of 5S rRNA associated with variable non quantitative amounts of one or more of 60S proteins L4, L15, L24, L31 and L41. The composition of the group of proteins released with 5S rRNA depends on both the molar ratio of EDTA and 60S subunits and the concentration of 60S subunits, in treatment mixtures.
D000818:Animals; D004492:Edetic Acid / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D012335:RNA, Ribosomal / Q000302:isolation & purification*; D012341:RNA, Ribosomal, 5S / Q000302:isolation & purification*; D012269:Ribosomal Proteins / Q000302:isolation & purification*; D012270:Ribosomes / Q000187:drug effects* / Q000648:ultrastructure; D013768:Tetrahymena / Q000235:genetics*
D012335:RNA, Ribosomal; D012341:RNA, Ribosomal, 5S; D012269:Ribosomal Proteins; D004492:Edetic Acid