4-Dimethylaminoazobenzene interferes with the binding of ribosomes to reticular membranes.
Treatment of chemically degranulated rough endoplasmic reticulum (RER) with 4-dimethylamino-azobenzene (4-DAB) partly damages binding of fresh polyribosomes to both the lighter and heavier fractions of reconstituted RER. Also, treatment of ribosomes with 4-DAB seemed to inhibit their binding to the chemically stripped RER (DRER) appearing both as lighter and heavier rough membrane layers after reconstitution. The interaction of 4-DAB with stripped microsomal fraction and ribosomes might be introducing certain conformational constraints into their proteins, thus crippling the binding of the ribosomes to reticular membranes.
D000818:Animals; D001665:Binding Sites / Q000187:drug effects; D004721:Endoplasmic Reticulum / Q000187:drug effects* / Q000378:metabolism; D066298:In Vitro Techniques; D007425:Intracellular Membranes / Q000187:drug effects; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012270:Ribosomes / Q000187:drug effects* / Q000378:metabolism; D004124:p-Dimethylaminoazobenzene / Q000494:pharmacology*
D004124:p-Dimethylaminoazobenzene