Prostaglandin-mediated suppression of macrophage phagocytosis of Listeria monocytogenes.
Suppression of macrophage phagocytosis of Listeria monocytogenes has been shown to be due to a low-molecular-weight component of spleen cell culture supernatant. The possibility that the factor could be a prostaglandin was investigated. When murine peritoneal macrophages were treated with prostaglandin E2 (PGE2), L. monocytogenes was phagocytized at a rate comparable to that phagocytized when treated with a low-molecular-weight fraction of concanavalin A-generated spleen cell culture supernatant. Suppressive activity of the spleen cell culture supernatant was abrogated when supernatant was prepared in the presence of indomethacin, a prostaglandin synthetase inhibitor. Prostaglandins were identified in supernatants with thin-layer and high-pressure liquid chromatography. These results suggest a role for prostaglandins, particularly PGE2, as a modulator of macrophage phagocytosis of L. monocytogenes.
D000818:Animals; D002478:Cells, Cultured; D003864:Depression, Chemical; D015232:Dinoprostone; D005260:Female; D007213:Indomethacin / Q000494:pharmacology; D008089:Listeria monocytogenes; D008214:Lymphocytes / Q000378:metabolism; D008222:Lymphokines / Q000378:metabolism / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000502:physiology*; D008297:Male; D051379:Mice; D010587:Phagocytosis / Q000187:drug effects*; D011453:Prostaglandins / Q000302:isolation & purification / Q000494:pharmacology*; D011458:Prostaglandins E / Q000494:pharmacology; D013154:Spleen / Q000166:cytology
D008222:Lymphokines; D011453:Prostaglandins; D011458:Prostaglandins E; D015232:Dinoprostone; D007213:Indomethacin