title: Relaxation of canine coronary artery to electrical stimulation: limited role of free radicals.
abstract: Electrical stimulation induces tetrodotoxin-insensitive relaxation of the canine coronary artery. The present study was designed to verify whether this relaxation involves the production of oxygen-derived free radicals. Isolated rings of canine coronary arteries were suspended for isometric tension recording in organ chambers filled with Krebs-Ringer bicarbonate solution. They were stimulated electrically (9 V, 3 Hz, 2 ms for 2 min) by means of two platinum electrodes during contractions evoked by various vasoactive agonists. Under control conditions, electrical stimulation caused rapid, reversible relaxations. Superoxide dismutase in association with catalase or mannitol, sodium ascorbate, dimethyl sulfoxide, and glutathione did not inhibit the relaxation caused by stimulation applied for only 2 min; neither did the removal of chloride ions from the salt solution nor the association of Cl-free solution in the presence of mannitol, superoxide dismutase, and catalase. Prolonging the electrical stimulation (9 V, 3 Hz, 2 ms) for up to 20 min produced a secondary relaxation. This second phase was inhibited by sodium ascorbate. These experiments indicate that the rapid relaxation induced by short-lasting electrical stimulation is probably not due to the generation of oxygen-derived free radicals. However, prolonged stimulation causes the production of such radicals, which then evoke irreversible inhibition of the vascular smooth muscle of the canine coronary artery.
mesh_terms: D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology; D002374:Catalase / Q000378:metabolism; D003331:Coronary Vessels / Q000502:physiology*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D015237:Dinoprost; D004285:Dogs; D004558:Electric Stimulation; D004594:Electrophysiology; D005260:Female; D005609:Free Radicals; D005978:Glutathione / Q000494:pharmacology; D006632:Histamine / Q000494:pharmacology; D008297:Male; D008353:Mannitol / Q000494:pharmacology; D010656:Phenylephrine / Q000494:pharmacology; D011189:Potassium Chloride / Q000494:pharmacology; D011460:Prostaglandins F / Q000494:pharmacology; D013482:Superoxide Dismutase / Q000378:metabolism; D014661:Vasoconstriction / Q000187:drug effects; D014664:Vasodilation
chemical_list: D005609:Free Radicals; D011460:Prostaglandins F; D010656:Phenylephrine; D008353:Mannitol; D011189:Potassium Chloride; D006632:Histamine; D015237:Dinoprost; D002374:Catalase; D013482:Superoxide Dismutase; D005978:Glutathione; D001205:Ascorbic Acid; D004121:Dimethyl Sulfoxide