title: Eicosanoid synthesis in 7,12-dimethylbenz(a)anthracene-induced mammary carcinomas in Sprague-Dawley rats fed primrose oil, menhaden oil or corn oil diet.
abstract: The comparative effects of high-fat diets (20%, w/w) on eicosanoid synthesis during mammary tumor promotion in 7,12-dimethylbenz(a)anthracene (DMBA)-induced rats were studied using diets containing 20% primrose oil (PO), 20% menhaden oil (MO) or 20% corn oil (CO). Sprague-Dawley rats fed the PO or MO diet had 21% of 24% fewer adenocarcinomas, respectively, than rats fed the CO diet. Histologically (i.e., mitotic figures, inflammatory cell infiltration and necrosis), the CO-fed rats exhibited the highest frequency of changes within tumors. Plasma fatty acid composition was significantly altered by diet, reflecting the composition of the oils which were being fed. Only the plasma of PO-fed rats contained detectable levels of gamma-linolenic acid (GLA). Arachidonic acid (AA) levels were significantly higher (p less than 0.05) in PO-fed than in CO- or MO-fed rats. MO-fed rats had significantly higher levels of plasma palmitic acid, while palmitoleic, eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids were detected only in MO-fed rats. As expected, linoleic acid (LA) and AA levels were lower (p less than 0.05) in the MO-fed rats than in PO- or CO-fed groups. The plasma of the CO-fed rats contained significantly higher levels of oleic acid. Eicosanoid synthesis in mammary carcinomas of rats fed the 20%-fat diets was 2-10 times higher than in mammary fat pads of control rats.(ABSTRACT TRUNCATED AT 250 WORDS)
mesh_terms: D015127:9,10-Dimethyl-1,2-benzanthracene; D000818:Animals; D003314:Corn Oil / Q000494:pharmacology*; D004041:Dietary Fats / Q000494:pharmacology*; D004537:Eicosanoic Acids / Q000096:biosynthesis*; D005227:Fatty Acids / Q000097:blood; D005228:Fatty Acids, Essential / Q000494:pharmacology*; D005260:Female; D005395:Fish Oils / Q000494:pharmacology*; D008041:Linoleic Acids; D008325:Mammary Neoplasms, Experimental / Q000139:chemically induced / Q000378:metabolism*; D008954:Models, Biological; D031665:Oenothera biennis; D010938:Plant Oils / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D017965:gamma-Linolenic Acid
chemical_list: D004041:Dietary Fats; D004537:Eicosanoic Acids; D005227:Fatty Acids; D005228:Fatty Acids, Essential; D005395:Fish Oils; D008041:Linoleic Acids; D010938:Plant Oils; C033266:Menhaden oil; C028498:evening primrose oil; D015127:9,10-Dimethyl-1,2-benzanthracene; D017965:gamma-Linolenic Acid; D003314:Corn Oil