title: Dot enzyme-linked immunosorbent assay (dot ELISA) for antibodies to Encephalitozoon cuniculi.
abstract: A dot-ELISA procedure was developed to detect antibodies against Encephalitozoon cuniculi. Sera from 84 rabbits, 22 dogs, 18 squirrel monkeys and 200 mice were tested by dot-ELISA and most also were tested by immunofluorescence. Comparison of the two tests showed that there was excellent agreement of the results (Kappa values greater than or equal to 0.74) in all four species. Dot-ELISA is a simple, quantitative, rapid alternative to immunofluorescence when large numbers of serum samples must be evaluated.
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000032:analysis*; D016782:Apicomplexa / Q000276:immunology*; D004283:Dog Diseases / Q000175:diagnosis; D004285:Dogs; D009670:Encephalitozoon cuniculi / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D051379:Mice; D008992:Monkey Diseases / Q000175:diagnosis; D011528:Protozoan Infections / Q000175:diagnosis; D011529:Protozoan Infections, Animal; D011817:Rabbits; D012376:Rodent Diseases / Q000175:diagnosis; D012453:Saimiri
chemical_list: D000913:Antibodies, Protozoan