The release of radioactive arachidonate from lipids of red blood cell membranes: properties of membrane and serum enzymatic activities.
The release of [3H]arachidonate from the phospholipids of red blood cell membranes of rats has been studied. Membranes of red cells isolated using acid-citrate-dextrose and differential centrifugation showed an endogenous release of arachidonate at pH 7.4 in the presence of CaCl2. Membranes from red cells isolated using heparin and Percoll gradient centrifugation are better substrates for serum-mediated release of arachidonate. These experiments and results with inhibitors suggest that red blood cell and serum phospholipase A2 activities are responsible for this catabolism that may provide arachidonate for subsequent biosynthesis of eicosanoids.
D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism*; D002853:Chromatography, Liquid; D003902:Detergents; D004798:Enzymes / Q000097:blood; D004910:Erythrocyte Membrane / Q000201:enzymology / Q000378:metabolism*; D050356:Lipid Metabolism; D051381:Rats
D001095:Arachidonic Acids; D003902:Detergents; D004798:Enzymes; D016718:Arachidonic Acid