Chicken liver TGGCA protein purified by preparative mobility shift electrophoresis (PMSE) shows a 36.8 to 29.8 kd microheterogeneity.
The TGGCA protein, the chicken homologue of HeLa cell NF-I, was purified to homogeneity from liver tissue by a procedure which includes preparative mobility shift electrophoresis (PMSE) as the final step. PMSE was here adjusted for the isolation of the TGGCA protein, but can be used as a general method to characterize the protein moiety of specific DNA-binding proteins. The TGGCA protein is a family of 6 protein species, which show minor differences in molecular weight from 36.8kd to 29.8kd. This microheterogeneity differs from the size distribution reported for HeLa cell NF-I polypeptides. All species of the TGGCA protein bind identically to a synthetic DNA-binding site and appear to be highly related in primary structure. We discuss the possible functional importance of this microheterogeneity.
D000178:Acrylamides; D000818:Animals; D001667:Binding, Competitive; D001888:Boric Acids; D022762:CCAAT-Enhancer-Binding Proteins; D002645:Chickens; D004268:DNA-Binding Proteins / Q000302:isolation & purification* / Q000378:metabolism; D004492:Edetic Acid; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D008099:Liver / Q000032:analysis*; D008970:Molecular Weight; D051839:NFI Transcription Factors; D009687:Nuclear Proteins; D010446:Peptide Fragments / Q000302:isolation & purification; D012967:Sodium Dodecyl Sulfate; D014157:Transcription Factors; D014325:Tromethamine; D051840:Y-Box-Binding Protein 1
D000178:Acrylamides; D001888:Boric Acids; D022762:CCAAT-Enhancer-Binding Proteins; D004268:DNA-Binding Proteins; D051839:NFI Transcription Factors; D009687:Nuclear Proteins; D010446:Peptide Fragments; D014157:Transcription Factors; D051840:Y-Box-Binding Protein 1; C070856:YBX1 protein, human; D014325:Tromethamine; D012967:Sodium Dodecyl Sulfate; D004492:Edetic Acid; C032688:boric acid