A quantitative bioassay to measure the rate of respiration of isolated rat tooth pulp tissue.
The formation of carbon dioxide, expressed as milligrammes of carbon dioxide per gramme wet weight, was used to determine the rate of succinate-induced respiration in rat incisal dental pulp, after incubation in a phosphate buffer. Seven experimental parameters: (a) physical nature of the tissue; (b) mass of the rat; (c) pre-incubation time; (d) incubation time; (e) substrate concentration; (f) effect of pH; and (g) effects of technique on pH, were analysed; pH was a critical determinant for success with this assay.
D000818:Animals; D001681:Biological Assay / Q000379:methods*; D002245:Carbon Dioxide / Q000096:biosynthesis*; D046508:Culture Techniques; D003782:Dental Pulp / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007180:Incisor; D051381:Rats
D002245:Carbon Dioxide