[Fluorimetric determination of UDP-glucuronyltransferase and sulfotransferase activity in human blood cells].
Activity of UDP-glucuronosyl transferase and sulfotransferase, the enzymes participating in the second step of xenobiotics metabolism, was studied using 4-methyl umbellipherone in human granulocytes, thrombocytes and lymphocytes. A simple procedure is described for production of 3-phosphoadenosine-5-phosphosulfate coenzyme of sulfotransferase. The data obtained in the course of fluorimetric estimation of activity of these conjugases corresponded to the results of an isotope technique.
D001773:Blood Cells / Q000201:enzymology*; D001792:Blood Platelets / Q000201:enzymology; D004912:Erythrocytes / Q000201:enzymology; D005470:Fluorometry / Q000379:methods*; D014453:Glucuronosyltransferase / Q000097:blood*; D006098:Granulocytes / Q000201:enzymology; D006801:Humans; D008214:Lymphocytes / Q000201:enzymology; D015238:Sulfotransferases / Q000097:blood*
D014453:Glucuronosyltransferase; D015238:Sulfotransferases
Fliuorimetricheskoe opredelenie aktivnosti UDF-gliukuroniltransferazy i sul'fotransferazy v kletkakh krovi cheloveka.