Effect of lipid composition on activity of liposome-entrapped ampicillin against intracellular Listeria monocytogenes.
The effect of lipid composition on the intracellular antibacterial activity of ampicillin-containing liposomes was studied in vitro by using mouse peritoneal macrophages infected with Listeria monocytogenes. Two types of liposomes, a fluid type, consisting of cholesterol-phosphatidylcholine-phosphatidylserine (5:4:1), and a solid type, consisting of cholesterol-distearoylphosphatidylcholine-dipalmitoylphosphatidylglyc ero l (10:10:1), were used. Although the cellular uptake of both types of liposomes was similar, they differed with respect to the rate of intracellular degradation. A correlation was found between the relatively slow degradation of the solid liposomes and a delayed intracellular release of the encapsulated ampicillin, as reflected in absent or delayed intracellular killing of L. monocytogenes.
D000667:Ampicillin / Q000008:administration & dosage*; D000818:Animals; D002478:Cells, Cultured; D002784:Cholesterol; D004337:Drug Carriers; D005260:Female; D008081:Liposomes; D008089:Listeria monocytogenes / Q000187:drug effects*; D008264:Macrophages / Q000382:microbiology; D051379:Mice; D008810:Mice, Inbred C57BL; D010713:Phosphatidylcholines; D010715:Phosphatidylglycerols; D010718:Phosphatidylserines
D004337:Drug Carriers; D008081:Liposomes; D010713:Phosphatidylcholines; D010715:Phosphatidylglycerols; D010718:Phosphatidylserines; D000667:Ampicillin; D002784:Cholesterol; C010942:1,2-distearoyllecithin; C030345:1,2-dipalmitoylphosphatidylglycerol