[Physico-chemical and biological characteristics of Pseudomonas aeruginosa elastase].
Elastase isolated from P. aeruginosa clinical strain hydrolyzes elastin, casein, hemoglobin, ovalbumin, gelatin, fibrin, collagen. The optimum pH ensuring the activity of the enzyme is 7.8-8.0. Elastase shows maximum stability at pH 6.6-9.0. Heating at 80 degrees C for 10 minutes results in its practically complete inactivation. Elastase is a highly radiosensitive enzyme. Chelating agents and zinc, cobalt, mercury ions suppress its activity. Sodium and ammonium chlorides selectively inhibit the elastolytic, but not proteolytic activity of the enzyme. Elastase shows pronounced dermonecrotic and keratolytic action.
D000818:Animals; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003315:Cornea / Q000187:drug effects; D004790:Enzyme Induction / Q000528:radiation effects; D004795:Enzyme Stability; D005720:Gamma Rays; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007928:Lethal Dose 50; D051379:Mice; D010196:Pancreatic Elastase / Q000032:analysis* / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000528:radiation effects / Q000633:toxicity; D011506:Proteins / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000201:enzymology* / Q000472:pathogenicity / Q000528:radiation effects; D013379:Substrate Specificity; D013696:Temperature
D011506:Proteins; D010196:Pancreatic Elastase
Nekotorye fiziko-khimicheskie i biologicheskie kharakteristiki élastazy Pseudomonas aeruginosa.