Induction of intercellular adhesion molecule 1 on primary and continuous cell lines by pro-inflammatory cytokines. Regulation by pharmacologic agents and neutralizing antibodies.
The expression of intercellular adhesion molecule-1 (ICAM-1) on primary human fibroblasts, a human fibrosarcoma, chondrosarcoma, and adenocarcinoma cell line in response to IL-1, TNF-alpha, or IFN-gamma was studied using an ELISA with anti-ICAM-1 mAb. The induction of ICAM-1 by these cytokines was neutralized by cytokine-specific antisera as well as some steroids and the glycosylation inhibitor, tunicamycin. Cyclohexamide up-regulated the expression of ICAM-1 on chondrosarcoma cells but had little or no effect on carcinoma cells. These data indicate different mechanisms in the regulation and expression of ICAM-1 on the various cell types and provide some insight into the anti-inflammatory effects of some pharmacologic agents.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000502:physiology*; D000954:Antigens, Surface / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D001688:Biological Products / Q000037:antagonists & inhibitors / Q000276:immunology / Q000494:pharmacology*; D015815:Cell Adhesion Molecules; D002460:Cell Line; D016207:Cytokines; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology*; D007249:Inflammation / Q000209:etiology / Q000276:immunology*; D007371:Interferon-gamma / Q000276:immunology / Q000494:pharmacology; D007375:Interleukin-1 / Q000276:immunology / Q000494:pharmacology; D007700:Kinetics; D051379:Mice; D009500:Neutralization Tests; D011817:Rabbits; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D001688:Biological Products; D015815:Cell Adhesion Molecules; D016207:Cytokines; D007106:Immune Sera; D007375:Interleukin-1; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma