title: A human-derived probe, p82H, hybridizes to the centromeres of gorilla, chimpanzee, and orangutan.
abstract: A human-derived centromeric sequence, p82H, hybridizes to DNA from gorilla, chimpanzee, pygmy chimpanzee, and orangutan. On DNA blots, multimeric ladders based on 170 or 340 bp repeat units are seen. In metaphase chromosome preparations from these species, p82H hybridizes to the centromeric region of every chromosome. p82H forms less stable hybrids with DNA from the lion-tailed macaque and does not hybridize to DNA or chromosomes of the owl monkey or the mouse.
mesh_terms: D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D002503:Centromere / Q000502:physiology*; D002875:Chromosomes / Q000502:physiology*; D005260:Female; D006071:Gorilla gorilla / Q000235:genetics*; D000882:Haplorhini / Q000235:genetics*; D015186:Hominidae / Q000235:genetics*; D006801:Humans; D006822:Hybrid Cells / Q000166:cytology; D007621:Karyotyping; D007962:Leukocytes / Q000166:cytology; D008297:Male; D051379:Mice; D009693:Nucleic Acid Hybridization; D002679:Pan troglodytes / Q000235:genetics*; D011148:Pongo pygmaeus / Q000235:genetics*