Chemical cross-linking and its effect on fatty acid synthetase activity in intact chloroplasts from Euglena gracilis.
Intact chloroplasts were isolated from Euglena gracilis variety bacillaris, aliquots were exposed to several different chemical cross-linking reagents. The reagents penetrated the triple membrane of Euglena chloroplasts. This was shown by gradient acrylamide gel electrophoresis under denaturing conditions. The activity of the nonaggregated fatty acid synthetase of Euglena was located within the chloroplast stroma, and the effects of dimethylsuberimidate cross-linking on the activity of the enzyme system were examined. The acyl-carrier protein concentration in the chloroplast was measured at about 0.24 mM.
D000213:Acyl Carrier Protein / Q000032:analysis; D000818:Animals; D002736:Chloroplasts / Q000201:enzymology*; D003432:Cross-Linking Reagents / Q000494:pharmacology*; D003720:Densitometry; D004120:Dimethyl Suberimidate / Q000494:pharmacology; D004139:Dinitrofluorobenzene / Q000031:analogs & derivatives / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D005055:Euglena / Q000201:enzymology*; D064429:Fatty Acid Synthases / Q000378:metabolism*; D005976:Glutaral / Q000494:pharmacology; D008314:Malonates / Q000493:pharmacokinetics; D008970:Molecular Weight; D013388:Succinimides / Q000494:pharmacology
D000213:Acyl Carrier Protein; D003432:Cross-Linking Reagents; D008314:Malonates; D013388:Succinimides; D004120:Dimethyl Suberimidate; C018052:disuccinimidyl tartarate; C030290:malonic acid; D004139:Dinitrofluorobenzene; D064429:Fatty Acid Synthases; C002041:1,5-difluoro-2,4-dinitrobenzene; D005976:Glutaral