title: Closely linked DNA elements control the expression of the Sgs-5 glue protein gene in Drosophila.
abstract: cis-acting sequence regions involved in the regulation of Sgs-5 gene expression were mapped by testing DNA segments containing the Sgs-5 RNA coding region and various amounts of adjacent sequences for the ability to express Sgs-5 RNA. Following injection of the DNA segments into Drosophila embryos, expression of the gene was assayed in the salivary glands of the injected animals after they developed to third instar larvae, these somatically transformed individuals serving as an in vivo transient expression system. The information necessary for the expression of Sgs-5 is contained within 109 bp upstream and 69 bp downstream of the transcribed region. Somatic transformation experiments also show that some feature within the limits of a 1012-bp DNA segment containing the Sgs-5 RNA coding region derived from the Sgs-5 RNA null stock CA-2 must be responsible for the lack of transcription from this allele. The only DNA sequence differences between active and null alleles, within the 1012 bp, are seven single-base-pair substitutions between -84 bp and +175 bp relative to the RNA start site. One or a combination of these sites are likely contributors to the transcriptional inactivity of the Sgs-5CA2 allele.
mesh_terms: D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification; D004331:Drosophila melanogaster / Q000235:genetics*; D005786:Gene Expression Regulation; D005796:Genes; D008040:Genetic Linkage; D005967:Glue Proteins, Drosophila / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids; D012471:Salivary Proteins and Peptides / Q000235:genetics*
chemical_list: D005967:Glue Proteins, Drosophila; D012471:Salivary Proteins and Peptides; D004247:DNA