title: Video image analysis of behavior by microcomputer: categorization of turning and locomotion after 6-OHDA injection into the substantia nigra.
abstract: The automated video image analyzing system (VIAS) measures locomotion in meters and turning behavior of rats by digitized video image and microcomputer evaluation. To measure turning behavior the number of 1/4, 1/2, 3/4 and complete turns are recorded. The turns are classified in different diameter classes. To test the validity of this system rats were administered unilateral 6-OHDA lesions of the substantia nigra and were observed by human rater and VIAS in parallel. Correlation coefficients for the data obtained by both methods were r = 0.98 for locomotion, r = 0.93 for half turns with diameter greater than or equal to 30 cm. Recovery from lesion-induced turning of animals with striatal dopamine depletion of 81.4% to 87.2% was described in detail by VIAS with respect to the diameter of the turns. It is concluded that VIAS provides a potent tool for the analysis of turning behavior and locomotion.
mesh_terms: D000818:Animals; D001522:Behavior, Animal / Q000502:physiology*; D006892:Hydroxydopamines; D007091:Image Processing, Computer-Assisted; D008838:Microcomputers; D009043:Motor Activity; D009068:Movement; D016627:Oxidopamine; D051381:Rats; D013378:Substantia Nigra / Q000187:drug effects; D014743:Videotape Recording
chemical_list: D006892:Hydroxydopamines; D016627:Oxidopamine