Amino acid sequence surrounding the lipoic acid cofactor of bovine kidney 2-oxoglutarate dehydrogenase complex.
The 2-oxoglutarate dehydrogenase complex was succinylated using 2-oxo[5-14C]glutarate in the presence of N-ethylmaleimide to label the lipoic acid cofactor of the transuccinylase (E2) component. Following peptic digestion, 14C-lipoate-containing peptides were purified and subjected to automated Edman degradation and amino acid analysis. The amino acid sequence surrounding the lipoyllysine residue is reported.
D000595:Amino Acid Sequence; D000818:Animals; D002250:Carbon Radioisotopes; D002417:Cattle; D007655:Ketoglutarate Dehydrogenase Complex / Q000378:metabolism*; D007656:Ketoglutaric Acids / Q000378:metabolism; D007658:Ketone Oxidoreductases / Q000378:metabolism*; D007672:Kidney Cortex / Q000201:enzymology*; D010446:Peptide Fragments / Q000032:analysis; D008063:Thioctic Acid
D002250:Carbon Radioisotopes; D007656:Ketoglutaric Acids; D010446:Peptide Fragments; D008063:Thioctic Acid; D007658:Ketone Oxidoreductases; D007655:Ketoglutarate Dehydrogenase Complex