Cloning and expression of an alpha-acetolactate decarboxylase gene from Streptococcus lactis subsp. diacetylactis in Escherichia coli.
The Streptococcus lactis gene coding for alpha-acetolactate decarboxylase (ADC) was cloned in Escherichia coli. Subsequent subcloning in E. coli showed that the ADC gene was located within a 1.3-kilobase DNA fragment. The ADC gene was controlled by its own promoter. Gas chromatography showed that S. lactis and the transformed E. coli strains produced the two optical isomers of acetoin in different ratios.
D000093:Acetoin / Q000378:metabolism; D002262:Carboxy-Lyases / Q000235:genetics*; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005798:Genes, Bacterial; D007773:Lactates / Q000378:metabolism; D013294:Lactococcus lactis / Q000201:enzymology / Q000235:genetics*; D013237:Stereoisomerism
D007773:Lactates; C006359:alpha-acetolactate; D000093:Acetoin; D002262:Carboxy-Lyases; C023746:acetolactate decarboxylase