Reduced levels of histones H1o and H1b, and unaltered content of methylated DNA in rainbow trout hepatocellular carcinoma chromatin.
The levels of histone subtypes and DNA methylation of aflatoxin-induced rainbow trout hepatocellular carcinoma and adult liver nuclei were compared. The hepatocellular carcinoma nuclei were enriched in the ubiquitinated species of histone H2A and depleted in histones H1o and H1b. The 5-methylcytosine content and methylation patterns of the vitellogenin genes and the transcriptionally inactive TPG-3 protamine gene were not altered in the trout hepatocellular carcinoma DNA. Thus, undermethylation of DNA is not a general feature of chemically induced tumors in vivo.
D044503:5-Methylcytosine; D016604:Aflatoxin B1; D000348:Aflatoxins; D000818:Animals; D002843:Chromatin / Q000032:analysis*; D003596:Cytosine / Q000031:analogs & derivatives / Q000032:analysis; D004247:DNA / Q000378:metabolism*; D006657:Histones / Q000378:metabolism*; D008099:Liver / Q000378:metabolism; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced / Q000378:metabolism*; D008745:Methylation; D014158:Transcription, Genetic; D014337:Trout; D014819:Vitellogenins / Q000235:genetics
D000348:Aflatoxins; D002843:Chromatin; D006657:Histones; D014819:Vitellogenins; D044503:5-Methylcytosine; D003596:Cytosine; D004247:DNA; D016604:Aflatoxin B1