title: Survival of rough and smooth strains of Brucella abortus in bovine mammary gland macrophages.
abstract: Chronic bovine brucellosis is characterized by persistent infection of the mammary gland. The interaction of live Brucella abortus with bovine mammary gland macrophages was studied in vitro. Opsonization of smooth B abortus strain 2308 and rough strain 45/20 was required for phagocytosis by mammary gland macrophages. When opsonized with specific antiserum, strains 2308 and 45/20 stimulated a considerable oxidative burst when phagocytized by mammary gland macrophages. Intracellular survival rates for strain 2308 were significantly higher than those for strain 45/20. After being phagocytized, B abortus localized in phagosomes and phagolysosomes of mammary gland macrophages.
mesh_terms: D000818:Animals; D002003:Brucella abortus / Q000276:immunology / Q000502:physiology*; D002417:Cattle; D005260:Female; D008264:Macrophages / Q000276:immunology / Q000382:microbiology* / Q000648:ultrastructure; D008321:Mammary Glands, Animal / Q000166:cytology*; D008854:Microscopy, Electron; D009895:Opsonin Proteins; D010587:Phagocytosis; D010588:Phagosomes / Q000382:microbiology; D013997:Time Factors
chemical_list: D009895:Opsonin Proteins