[Comparative analysis of the DNA of Corynebacterium diphtheriae phages and the cloning of the gene determining diphtheria toxin synthesis].
The analysis of the DNA of one nontoxigenic C. diphtheriae phage and two toxigenic ones has revealed that phage phi 984tox+ belongs to omega-like tox+ phages, phage phi 9tox+ is a representative of a new group of phages and phage B (Freeman) tox is a deletion mutant of phage beta. The location of this deletion on the physical map of this phage has been established. To obtain the physical map of phage phi 984tox+, the complete library of internal DNA fragments has been constructed in vector pBR 322. The gene of native diphtheria toxin has been cloned in vectors pBR 322 and pUR 250. Plasmids pUR 250 with the inserts of the toxin gene have been shown to be unstable if tox and lac promoters are located in tandem before the body of the toxin gene. The prolonged cultivation of clones having such structure leads to the formation of a spontaneous mutation located in the region coding the C-end part of the A-fragment of the toxin.
D001435:Bacteriophages / Q000235:genetics*; D002872:Chromosome Deletion; D003001:Cloning, Molecular / Q000379:methods*; D003353:Corynebacterium diphtheriae; D004279:DNA, Viral / Q000235:genetics*; D004167:Diphtheria Toxin / Q000096:biosynthesis / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D005814:Genes, Viral; D005822:Genetic Vectors; D010957:Plasmids; D015183:Restriction Mapping
D004279:DNA, Viral; D004167:Diphtheria Toxin
Sravnitel'nyĭ analiz DNK fagov Corynebacterium diphtheriae i klonirovanie gena, determiniruiushchego sintez difteriĭnogo toksina.