[Changes in the intracellular concentration of calcium ions in isolated neurons of the edible snail detected by chlortetracycline fluorescence].
Chlorotetracycline fluorescence changes were studied in isolated snail (Helix pomatia) neurons. It was shown that the properties of chlorotetracycline fluorescence satisfy those necessary for Ca-sensitive probes. Chlorotetracycline fluorescence increased during membrane depolarization induced by K+ increase in the medium or by addition of veratridine. Both effects were absent in Ca-free solution or in Cd-containing medium. Caffeine also induced an increase in chlorotetracycline fluorescence. This effect is, probably, a result of Ca release from intracellular stores, because it does not depend on extracellular Ca.
D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D002751:Chlortetracycline / Q000493:pharmacokinetics*; D006372:Helix, Snails / Q000378:metabolism*; D008856:Microscopy, Fluorescence / Q000295:instrumentation; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*
D000001:Calcimycin; D002118:Calcium; D002751:Chlortetracycline
Izmeneniia vnutrikletochnoĭ kontsentratsii ionov kal'tsiia v izolirovannykh neĭronakh vinogradnoĭ ulitki, vyiavliaemye po fluorestsentsii khlortetratsiklina.