Pre-exposure of human B cells to recombinant IL-1 enhances subsequent proliferation.
The reported effects of the monocyte-derived cytokine IL-1 on human B lymphocytes are both varied and controversial. IL-1 has been reported to augment both proliferation and Ig secretion of previously activated human B cells. In the present study highly purified splenic B cells were cultured with rIL-1 before, simultaneously with, and after the addition of the polyclonal B cell mitogen, anti-Ig. rIL-1 had no significant effect on B cell proliferation when added simultaneously with or after B cell activation with anti-Ig. However, incubation of splenic B cells with rIL-1 for 24 h before stimulation with anti-Ig appeared to enhance mitogenesis. With the observation that rIL-1 exerted effects on resting B cells, the effect of rIL-1 on several events which accompany B cell activation was examined. rIL-1 failed to stimulate RNA synthesis, effect increases in cell size or intracellular Ca2+ levels, or lead to the hyperexpression of MHC class II or B cell activation Ag. These studies suggest that rIL-1 does not activate B cells but primes them to respond to subsequent activation.
D000888:Antibodies, Anti-Idiotypic; D001402:B-Lymphocytes / Q000187:drug effects* / Q000276:immunology; D002478:Cells, Cultured; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D007375:Interleukin-1 / Q000494:pharmacology*; D007376:Interleukin-2; D015847:Interleukin-4; D007378:Interleukins; D008213:Lymphocyte Activation / Q000187:drug effects*; D014066:Palatine Tonsil / Q000187:drug effects / Q000276:immunology; D011994:Recombinant Proteins / Q000494:pharmacology*; D013154:Spleen / Q000187:drug effects / Q000276:immunology; D013997:Time Factors
D000888:Antibodies, Anti-Idiotypic; D007375:Interleukin-1; D007376:Interleukin-2; D007378:Interleukins; D011994:Recombinant Proteins; D015847:Interleukin-4; D007371:Interferon-gamma