title: Differentiation of Mycobacterium thermoresistibile from Mycobacterium phlei and other rapidly growing mycobacteria.
abstract: Mycobacterium thermoresistibile is the only chromogenic rapidly growing mycobacterial species reported to cause infections in humans and animals. DNA-DNA hybridization (S1 nuclease method) showed that M. thermoresistibile formed a DNA relatedness group which was only 24 to 30% related to M. phlei. Alkaline phosphatase and beta-galactosidase differentiated M. thermoresistibile from M. phlei. Among the rapidly growing mycobacteria, the mycolic acid pattern of M. thermoresistibile was unique (alpha-, alpha'-, methoxy-and keto-mycolates). Fourteen other species of chromogenic rapidly growing mycobacteria, including M. phlei, produced different mycolic acid patterns which always included dicarboxylic mycolate. Nine species of non-chromogenic rapidly growing mycobacteria produced mycolic acid patterns devoid of ketomycolate.
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis; D001669:Biochemical Phenomena; D001671:Biochemistry; D003470:Culture Media; D004269:DNA, Bacterial; D009161:Mycobacterium / Q000302:isolation & purification* / Q000378:metabolism; D009168:Mycobacterium phlei / Q000032:analysis / Q000302:isolation & purification; D009171:Mycolic Acids / Q000032:analysis; D009693:Nucleic Acid Hybridization; D010405:Penicillinase / Q000032:analysis; D001616:beta-Galactosidase / Q000032:analysis; D001617:beta-Glucosidase / Q000032:analysis
chemical_list: D003470:Culture Media; D004269:DNA, Bacterial; D009171:Mycolic Acids; D000469:Alkaline Phosphatase; D001617:beta-Glucosidase; D001616:beta-Galactosidase; D010405:Penicillinase