[Hemolytic effect of phospholipase A2 and orientotoxin from venom of the great hornet, Vespa orientalis].
The effect of toxic phospholipase A2 and orientotoxin from the venom of the giant hornet Vespa orientalis on human erythrocytes was studied. It was shown that these venom components are potent hemolytic agents, the efficiency of the latter being by about two orders of magnitude as high as that of phospholipase A2. The hemolytic function of the both components is enhanced in the presence of low concentrations of Ca2+, whereas high concentrations of this cation exert an inhibiting action. Polyvalent cations, in particular, ruthenium red, peptide HR-1, mellitin and cytotoxins Us-1 and Us-5 synergetically increase the hemolytic effect of phospholipase A2. During erythrocyte hemolysis the synergistic effect is manifested upon a combined action of phospholipase A2 and orientotoxin. The combination of these toxins increases the total hemolytic activity and produces a far greater effect than in could be expected in the case of each of these compounds taken separately.
D001514:Bee Venoms / Q000633:toxicity*; D002118:Calcium / Q000494:pharmacology; D004357:Drug Synergism; D004492:Edetic Acid / Q000494:pharmacology; D004912:Erythrocytes / Q000378:metabolism; D006461:Hemolysis / Q000187:drug effects*; D006801:Humans; D066298:In Vitro Techniques; D010740:Phospholipases / Q000633:toxicity*; D010741:Phospholipases A / Q000037:antagonists & inhibitors / Q000633:toxicity*; D054467:Phospholipases A2; D011188:Potassium / Q000097:blood; D014862:Wasp Venoms / Q000037:antagonists & inhibitors / Q000633:toxicity*
D001514:Bee Venoms; D014862:Wasp Venoms; C042190:orientotoxin; D004492:Edetic Acid; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2; D011188:Potassium; D002118:Calcium
Gemoliticheskiĭ éffekt fosfolipazy A2 i orientotoksina iz iada bol'shogo shershnia Vespa orientalis.