Luminal and metabolic regulation of jejunal amino acid absorption in the rat.
Studies were carried out to determine the role of luminal amino acids and metabolic balance in in vivo amino acid absorption. Previous in vitro studies have shown that adaptation of amino acid transport is a complex phenomenon. In the first series of experiments, parenterally nourished rats received a 7-day jejunal infusion of either 3% aspartic acid, glutamine, lysine, valine, or mixed amino acids. A single-pass perfusion was performed to determine the effects of infusates on 5 mM valine, aspartic acid, and lysine absorption. Compared with controls receiving luminal saline, prior glutamine infusion increased valine absorption; prior valine, glutamine, and aspartic acid infusion significantly increased aspartic acid absorption; and prior valine and lysine infusion significantly increased lysine absorption. The mixed amino acid solution had no effect. The effects of metabolic balance were examined by comparing fasted rats with parenterally fed and orally fed rats. Within 24 h fasting significantly increased valine and aspartic acid absorption, despite a significant decrease in intestinal mass.
D000284:Administration, Oral; D000596:Amino Acids / Q000008:administration & dosage / Q000378:metabolism* / Q000494:pharmacology; D000818:Animals; D001224:Aspartic Acid / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology; D001835:Body Weight; D005215:Fasting; D005973:Glutamine / Q000008:administration & dosage / Q000494:pharmacology; D007408:Intestinal Absorption; D007413:Intestinal Mucosa / Q000166:cytology; D007583:Jejunum / Q000378:metabolism*; D008239:Lysine / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology; D008297:Male; D010289:Parenteral Nutrition, Total; D051381:Rats; D011919:Rats, Inbred Strains; D014633:Valine / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology
D000596:Amino Acids; D005973:Glutamine; D001224:Aspartic Acid; D014633:Valine; D008239:Lysine