Studies on the interference of selected substances with two modifications of the Lowry protein determination.
Two modifications of Lowry's procedure (Markwell's and Kresze's) were investigated for interference by zwitterionic buffers) (HEPES, HEPPS, PIPES, MES, MOPS, CHES, CAPS, Tris, glycylglycine, bicine), detergents (Triton-X-100, digitonin), sugars (sucrose, mannitol, sorbitol), salts (NaCl, KCl, ammonium acetate), chelators (EDTA), and reducing agents (dithiothreitol, GSH, ascorbic acid). The degree and type of interference are presented, and possible means of elimination of interference are discussed.
D002021:Buffers / Q000494:pharmacology; D002241:Carbohydrates / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D010084:Oxidation-Reduction; D012016:Reference Values; D012492:Salts / Q000494:pharmacology; D012710:Serum Albumin, Bovine / Q000032:analysis*
D002021:Buffers; D002241:Carbohydrates; D012492:Salts; D012710:Serum Albumin, Bovine; D004492:Edetic Acid