title: Identification and characterization of the major proteins of mammalian brain synaptic vesicles.
abstract: Highly purified rat and cow brain synaptic vesicles contain major proteins with molecular weights of approximately 74,000, 60,000, 57,000, 40,000, 38,000, and 34,000 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The presence of the major proteins on synaptic vesicles was confirmed by immunoprecipitation of intact rat brain synaptic vesicles with a synaptic vesicle-specific monoclonal antibody. The 40,000-Mr protein appeared to be identical to the 38,000-Mr integral membrane glycoprotein, p38 or synaptophysin, previously identified as a major component of mammalian synaptic vesicles. The isoelectric point of the 75,000-Mr proteins from either rat or cow brain synaptic vesicles is 5.0, and the pI of the 57,000-Mr protein is approximately 5.1 in both species. The similarity in size and charge of several major proteins in rat and cow synaptic vesicles suggests a high degree of structure conservation of these proteins in diverse mammalian species and raises the possibility that a set of functions common to most or all mammalian synaptic vesicles is mediated by these proteins.
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D001921:Brain / Q000648:ultrastructure*; D002417:Cattle; D003902:Detergents; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000032:analysis; D007163:Immunosorbent Techniques; D007526:Isoelectric Point; D008565:Membrane Proteins / Q000032:analysis; D008861:Microsomes / Q000032:analysis; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000032:analysis*; D051381:Rats; D013572:Synaptic Vesicles / Q000032:analysis* / Q000648:ultrastructure; D016708:Synaptophysin
chemical_list: D000911:Antibodies, Monoclonal; D003902:Detergents; D006023:Glycoproteins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D016708:Synaptophysin