Storage of sialic acid-containing carbohydrates in the placenta of a human galactosialidosis fetus. Isolation and structural characterization of 16 sialyloligosaccharides.
From the placenta of a human fetus with galactosialidosis, detected by prenatal diagnosis, sialyloligosaccharides were isolated by successively gel-permeation chromatography on Bio-Gel P-6, anion-exchange chromatography on Mono Q and high-performance liquid chromatography on Lichrosorb-NH2. 16 sialic acid-containing N-glycosidic N-acetyllactosamine type of structures were identified by sugar analysis and 500-MHz 1H-NMR spectroscopy. The fully sialylated oligosaccharides differ from each other in the type of branching (mono-, di-, tri-, tri'- and tetra-antennary) or sialic acid linkage types (alpha 2-3/alpha 2-6). The isolated carbohydrates, including six novel structures, can be presented as follows (the numerals in a column represents one compound). (formula; see text).
D002236:Carbohydrate Conformation; D050260:Carbohydrate Metabolism; D002240:Carbohydrate Sequence; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D005333:Fetus / Q000378:metabolism*; D005696:Galactosidases / Q000172:deficiency*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009439:Neuraminidase / Q000172:deficiency*; D009844:Oligosaccharides / Q000302:isolation & purification*; D010920:Placenta / Q000032:analysis* / Q000378:metabolism; D012794:Sialic Acids / Q000378:metabolism*; D001616:beta-Galactosidase / Q000172:deficiency*
D009844:Oligosaccharides; D012794:Sialic Acids; C016735:sialooligosaccharides; D005696:Galactosidases; D009439:Neuraminidase; D001616:beta-Galactosidase