[The relationship of the occurrence and growth of experimental cervical carcinoma to the local sympathetic nervous system].
An experiment was carried out to clarify the relationship of the occurrence and growth of experimental cervical carcinoma to its surrounding sympathetic nerves. We first examined the effect of sympathetic nerve disturbance on the occurrence and growth of tumors. Using 20-methylcholanthrene (MC), cervical carcinoma was induced in mice, in which the sympathetic nervous system was blocked with 6-hydroxydopamine (6-OH-DA) while a control group received physiological saline. After 16 weeks, the incidence of invasive tumor in the uterine cervix (cervix), the size of the tumor, and the site where the tumor was most likely to occur were compared in two groups. Although the 6-OH-DA group tended to bear a larger tumor, there was no difference in the incidence of invasive tumor or the site where the tumor was most likely to occur. Next, the localization of the sympathetic neurotransmitter norepinephrine (NE) in the cervix was estimated by the fluorescent histochemical method 16 weeks after the insertion of a MC-impregnated thread to determine the effect of cervical carcinoma on sympathetic nervous function in the surrounding tissue. The intensity of fluorescence was reduced around the tumor, and no fluorescence was detected within the tumor. These results were confirmed by the measurement of NE content in the cervix with HPLC. Based on these results, it was revealed that sympathetic nervous disturbance facilitated the growth of experimental cervical carcinoma without influencing its occurrence, and that the sympathetic nerves in the surrounding tissue to cervical carcinoma were damaged by some mechanism of action.
D000818:Animals; D002584:Cervix Uteri / Q000294:innervation; D005260:Female; D006892:Hydroxydopamines; D008748:Methylcholanthrene; D051379:Mice; D009361:Neoplasm Invasiveness; D009638:Norepinephrine / Q000032:analysis; D016627:Oxidopamine; D013564:Sympathetic Nervous System / Q000032:analysis / Q000503:physiopathology*; D002583:Uterine Cervical Neoplasms / Q000139:chemically induced / Q000473:pathology*
D006892:Hydroxydopamines; D008748:Methylcholanthrene; D016627:Oxidopamine; D009638:Norepinephrine