Presence of a cellular retinylphosphate binding protein in rat liver.
A retinylphosphate binding activity, resolved during purification, has been discovered in rat liver cytosol. The partial purification includes ammonium sulfate precipitation and DEAE-cellulose chromatography. The macromolecular component responsible for the binding has a sedimentation coefficient of about 2 S and is sensitive to pronase. This binding is reversible and specific for retinylphosphate, since retinol, retinoic acid and retinoylphosphate do not compete with [3H]retinylphosphate.
D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D002848:Chromatography, DEAE-Cellulose; D002855:Chromatography, Thin Layer; D004224:Diterpenes; D004591:Electrophoresis, Polyacrylamide Gel; D008024:Ligands; D008099:Liver / Q000032:analysis*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D014801:Vitamin A / Q000031:analogs & derivatives* / Q000378:metabolism
D002352:Carrier Proteins; D004224:Diterpenes; D008024:Ligands; D014801:Vitamin A; C012532:retinol phosphate