Effects of ammoniation on the 'carry-over' of aflatoxins into bovine milk.
Two experiments were performed using lactating cows fed various treated and non-treated commodities from AFB1 contaminated peanut cakes. Treatment with ammonia gas by an autoclaving process was used for detoxification. Two methods were used for AFM1 determination in every milk sample: a TLC procedure recognized by AOAC and IDF and an HPLC method with a detection limit of 0.100 and 0.010 microgram/l, respectively. In a first experiment, lactating cows were fed treated and untreated meals during periods separated by uncontaminated soya meals phases. The total excreted AFM1 was 2.6% of the total ingested AFB1 from untreated feed contaminated at 1100 micrograms/kg. During periods receiving treated meals in the diet, AFM1 contents in milk were below 0.1 microgram/l. However, by using AFM1 data obtained using the HPLC method, an AFM1/AFB1 ratio of 4.6% was found from treated feed contaminated at 40 micrograms AFB1/kg. In a second experiment, a herd of 50 lactating cows was used for a long term (16 months) feeding of mixed commodities containing 30% ammoniated peanut cakes. AFB1 residues in the treated diet were below 10 micrograms/kg, the EEC action level, and no AFM1 residue was found up to 0.1 microgram/l in collected milk throughout this experiment.
D016604:Aflatoxin B1; D016607:Aflatoxin M1; D000348:Aflatoxins / Q000032:analysis*; D000641:Ammonia; D000821:Animal Feed; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D005506:Food Contamination / Q000032:analysis / Q000517:prevention & control*; D008892:Milk / Q000032:analysis*
D000348:Aflatoxins; D016607:Aflatoxin M1; D000641:Ammonia; D016604:Aflatoxin B1