title: An experiment in enzyme evolution. Studies with Pseudomonas aeruginosa amidase.
abstract: The regulation of amidase synthesis in P. aeruginosa is under positive control. This review describes the experimental evolution of amidase and its regulator protein for the hydrolysis of novel substrates and experiments to elucidate the mechanism of the control system.
mesh_terms: D000577:Amides; D000581:Amidohydrolases / Q000096:biosynthesis / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D005075:Biological Evolution; D003001:Cloning, Molecular; D003470:Culture Media; D005809:Genes, Regulator; D008969:Molecular Sequence Data; D009154:Mutation; D011550:Pseudomonas aeruginosa / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D013379:Substrate Specificity
chemical_list: D000577:Amides; D003470:Culture Media; C006866:butyramide; D000581:Amidohydrolases