title: Systemic exchangeability of enteral leucine: relationship to plasma flux.
abstract: The exchangeability of enterally infused leucine within the systemic compartment was estimated in fasted and fed rats using L-[1-14C]leucine as a tracer. The experimental design consisted of enteral and parenteral feedings with intravenous or intragastric tracer infusions. During continuous intragastric feeding, only 73 +/- 6% (SE) of the intragastric leucine tracer infusion was accounted for in the systemic circulation. When comparing intravenous vs. intragastric tracer, the estimate of the contribution of protein breakdown to plasma leucine flux was 6 +/- 1 (SE) mumol.h-1.100 g-1 and 18 +/- 3 (SE) mumol.h-1.100 g-1 (P less than 0.01), respectively, for the two routes of administration. Correction of enteral input (either isotope or total leucine), by a factor of 27% for first-pass extraction, eliminated all significant differences in plasma leucine kinetics. Of the 27% of enterally infused tracer not appearing systemically, only 3% could be accounted for in newly synthesized protein in the liver. The remainder is hypothesized to represent first-pass utilization of leucine in intestinal protein synthesis and other metabolic pathways in the splanchnic bed. In contrast, systemic appearance of enteral leucine was essentially complete in the fasted rats, indicating less splanchnic metabolism of leucine in this state. These data indicate that significant error can result in estimating the contribution of endogenous protein breakdown to plasma leucine flux during feeding if the systemic exchangeability of dietary leucine is not considered.(ABSTRACT TRUNCATED AT 250 WORDS)
mesh_terms: D000818:Animals; D002250:Carbon Radioisotopes; D004435:Eating; D004750:Enteral Nutrition; D005215:Fasting; D007930:Leucine / Q000008:administration & dosage / Q000097:blood / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008297:Male; D008433:Mathematics; D008954:Models, Biological; D009132:Muscles / Q000378:metabolism*; D011865:Radioisotope Dilution Technique; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D002250:Carbon Radioisotopes; D007930:Leucine