Preliminary report on microencapsulated islet transplantation in experimental diabetes mellitus in China.
Pancreatic islets were encapsulated with sodium alginate-polylysine after procurement by collagenase digestion and Ficoll density-gradient-dispersion. The encapsulated islets were intact and during the first week of culture produced insulin 63 +/- 42 microU/d vs 69 +/- 42 microU/d by noncapsulated islets. Seven streptozotocin-induced diabetic rats each received 4.0-4.5 x 10(3) microencapsulated islets intraperitoneally. After transplantation, blood glucose dropped from 350 +/- 29 mg/dl to 142 +/- 12 mg/dl. To date, normoglycemia has been maintained for 222 days. Control animals either died or developed cataracts.
D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D002214:Capsules; D002681:China; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000473:pathology / Q000601:surgery*; D007515:Islets of Langerhans / Q000033:anatomy & histology; D016381:Islets of Langerhans Transplantation; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D001786:Blood Glucose; D002214:Capsules