Mouse strain differences in the induction of micronuclei by polycyclic aromatic hydrocarbons.
The frequency of micronucleated erythrocytes (MNE) in 3 inbred mouse strains and 2 of their hybrids (C57BL/6, BALB/c, DBA/2, BDF1 and CDF1) were examined after polycyclic aromatic hydrocarbons (PAHs; 7,12-dimethylbenz[a]anthracene (DMBA), 3-methylcholanthrene (3-MC), benzo[a]pyrene (BaP), benzo[e]pyrene (BeP) and anthracene (ANT] were injected i.p. PAHs are thought to form active metabolites after being administered to mammals. In mouse strains with inducible PAH activating enzymes, such as C57BL/6 or BALB/c, MNE were significantly induced, as compared to control mice, 48 h after DMBA, BaP, or 3-MC was injected. No increase in the frequency of MNE occurred in the DBA/2 strain which cannot induce the activating enzymes. BeP and ANT did not increase the frequency of MNE in any mouse used. The levels of MNE induction in BDF1 or CDF1 hybrids were similar to those in C57BL/6 or BALB/c. These results support the view that the genetic capacity to metabolize PAHs is strongly associated with micronucleus induction as in the case of PAH carcinogenesis.
D000818:Animals; D001711:Biotransformation; D001853:Bone Marrow / Q000187:drug effects; D002467:Cell Nucleus / Q000648:ultrastructure; D002869:Chromosome Aberrations; D003520:Cyclophosphamide / Q000633:toxicity; D051379:Mice; D008815:Mice, Inbred Strains; D016685:Mitomycin; D008937:Mitomycins / Q000633:toxicity; D009152:Mutagenicity Tests / Q000379:methods*; D011083:Polycyclic Compounds / Q000633:toxicity*; D013852:Thiotepa / Q000633:toxicity
D008937:Mitomycins; D011083:Polycyclic Compounds; D016685:Mitomycin; D003520:Cyclophosphamide; D013852:Thiotepa