Characterization of amylase linked immunoglobulin G to distinguish human salivary and pancreatic isoamylases.
An immunoglobulin G of the kappa type linked to salivary amylase was identified in the serum of a patient with colon cancer and persistent hyperamylasemia. The binding site on the immunoglobulin in the complex is located in F(ab')2 portion. The purified IgG recombined only with purified human salivary amylase, and could be used to separate human isoamylases.
D000918:Antibody Specificity; D002846:Chromatography, Affinity; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology*; D007145:Immunoglobulin kappa-Chains / Q000276:immunology; D007527:Isoenzymes / Q000276:immunology* / Q000302:isolation & purification; D010179:Pancreas / Q000201:enzymology; D012469:Salivary Glands / Q000201:enzymology; D000516:alpha-Amylases / Q000276:immunology* / Q000302:isolation & purification
D007074:Immunoglobulin G; D007145:Immunoglobulin kappa-Chains; D007527:Isoenzymes; D000516:alpha-Amylases