Maternal erythrocytes in the fetal circulation. The immunocytochemical identification of minor populations of erythrocytes.
Maternal erythrocytes (Rh+) have been identified in the fetal circulation of Rh- infants by the use of a simple immunocytochemical staining technic. Placental or capillary blood from an Rh- infant of an Rh+ mother was incubated in sequence with Rh immunoglobulin, rabbit antihuman IgG, and finally goat antirabbit IgG-alkaline phosphatase conjugate. Maternal cells were found in 14% (11 of 80) of placental vein samples and 10% (2 of 20) of capillary samples. The volume of maternal blood present in the fetal circulation was calculated to be 0.06-2.3 mL with the use of placental vein samples and 0.6-1.25 mL with the use of capillary blood.
D002196:Capillaries; D004912:Erythrocytes / Q000032:analysis; D005260:Female; D005312:Fetal Blood / Q000166:cytology*; D006801:Humans; D007231:Infant, Newborn; D008431:Maternal-Fetal Exchange; D010920:Placenta / Q000098:blood supply; D011247:Pregnancy; D012204:Rh-Hr Blood-Group System / Q000032:analysis; D014680:Veins
D012204:Rh-Hr Blood-Group System