[In vitro studies on microbial incorporation of nitrogen from [15N2] urea and [15N]ammonium chloride by human intestinal flora].
6 typical bacteria species of the human intestinal flora (E. coli, Klebsiella pneumoniae, Proteus vulgaris, Streptococcus faecalis, Bacteroides fragilis, Bifidobacterium sp.) were incubated in a liquid medium for 48 h with [15N2]-urea and [15N]-ammonium chloride. The rates of [15N]-incorporation were calculated. They depend reproducible on the species examined, on the kind of the offered NPN-substance and on the amount of NPN-substance in the medium. With [15N2]-urea the minimal rate of incorporation was 3.8% (E coli) and the maximal one 95.6% (Bifidobacterium sp.). With [15N]-ammonium chloride the corresponding figures were 31.0 (Proteus vulg.) and 98.0% (Bifidobacterium sp.). The findings are discussed with regard to a possible enteral detoxification in uremic patients by bacterial utilization and elimination of urea and ammonia.
D000643:Ammonium Chloride / Q000378:metabolism*; D001419:Bacteria / Q000378:metabolism*; D001441:Bacteroides fragilis / Q000378:metabolism; D001644:Bifidobacterium / Q000378:metabolism; D013293:Enterococcus faecalis / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism; D006801:Humans; D007422:Intestines / Q000382:microbiology*; D007711:Klebsiella pneumoniae / Q000378:metabolism; D009587:Nitrogen Isotopes; D011514:Proteus vulgaris / Q000378:metabolism; D014508:Urea / Q000378:metabolism*
D009587:Nitrogen Isotopes; D000643:Ammonium Chloride; D014508:Urea
In-vitro-Untersuchungen zur mikrobiellen Inkorporation von Stickstoff aus [15N2]Harnstoff und [15N2]Ammoniumchlorid durch die Intestinalflora des Menschen.