Human urokinase-type plasminogen activator stimulates chemotaxis of human neutrophils.
Human purified urokinase-type plasminogen activator (u-PA) stimulates chemoattractant activity for human neutrophils using modified Boyden chambers. Checkerboard analysis performed by adding different concentrations of u-PA above and below the polycarbonate filters revealed maximum migration required a positive concentration gradient. These results suggest that uPA was in fact stimulating neutrophil chemotaxis. Incubation of u-PA with an anti-u-PA goat antibody completely abolished the chemotactic activity of u-PA while incubation with the serine protease inhibitor, diisopropyl fluorophosphate, did not reduce chemotactic activity. Purified human tissue-type plasminogen activator demonstrated no chemotactic activity for human neutrophils when tested at concentrations similar to u-PA. These results suggests that the expression of chemotactic activity of u-PA may serve to recruit circulating leukocytes to the inflammatory site.
D002634:Chemotaxis, Leukocyte; D006801:Humans; D007158:Immunologic Techniques; D066298:In Vitro Techniques; D007531:Isoflurophate / Q000494:pharmacology; D009504:Neutrophils / Q000502:physiology*; D010959:Tissue Plasminogen Activator / Q000502:physiology; D014568:Urokinase-Type Plasminogen Activator / Q000502:physiology*
D007531:Isoflurophate; D010959:Tissue Plasminogen Activator; D014568:Urokinase-Type Plasminogen Activator