Serum-induced arachidonic acid release and prostaglandin biosynthesis are potentiated by oxygenated sterols in NRK 49F cells.
Fetal calf serum is able to activate arachidonic acid release from phospholipids in NRK 49F cells. We showed that this phenomenon can be potentiated by adding oxysterols to the culture medium. The oxysterol effect was dose-dependent and was not observed in the absence of fetal calf serum. Greater amounts of prostaglandin E2 and prostaglandin F2 alpha were released into the medium in the presence of oxysterols without apparent modification of the cyclooxygenase activity. The most effective oxysterols, in descending order, were the following: calcitriol greater than 7 alpha-hydroxycholesterol greater than 7 beta-hydroxycholesterol greater than 25-hydroxycholesterol. Cholesterol and 7-ketocholesterol were unable to activate phospholipase activity. The mechanism of this activation by oxysterols is still unknown.
D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism*; D001769:Blood; D002250:Carbon Radioisotopes; D002417:Cattle; D002460:Cell Line; D002855:Chromatography, Thin Layer; D015237:Dinoprost; D015232:Dinoprostone; D005312:Fetal Blood; D006888:Hydroxycholesterols / Q000494:pharmacology*; D006893:Hydroxyeicosatetraenoic Acids / Q000096:biosynthesis; D007700:Kinetics; D010743:Phospholipids / Q000378:metabolism; D015230:Prostaglandin D2; D011453:Prostaglandins / Q000096:biosynthesis*; D011454:Prostaglandins A / Q000096:biosynthesis; D011457:Prostaglandins D / Q000096:biosynthesis; D011458:Prostaglandins E / Q000096:biosynthesis; D011460:Prostaglandins F / Q000096:biosynthesis
D001095:Arachidonic Acids; D002250:Carbon Radioisotopes; D006888:Hydroxycholesterols; D006893:Hydroxyeicosatetraenoic Acids; D010743:Phospholipids; D011453:Prostaglandins; D011454:Prostaglandins A; D011457:Prostaglandins D; D011458:Prostaglandins E; D011460:Prostaglandins F; D016718:Arachidonic Acid; D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; C007997:25-hydroxycholesterol; D015237:Dinoprost; C100008:prostaglandin A2; D015232:Dinoprostone; D015230:Prostaglandin D2