[Cloning in cells of Bacillus subtilis and study of the characteristics of promoter regions of DNA of Bacillus mesentericus and Phage SPO2].
11 fragments of Bacillus mesentericus and phage SPO1 DNA containing promoters were cloned in Bacillus subtilis. Nucleotide sequences of these fragments were determined and S1 mapping of transcriptional start points was performed. It was found that some fragments contained tandem or overlapping promoters. Nucleotide sequences of Bacillus mesentericus promoters were compared with known promoters from bacilli. Promoter activities were determined in different phases of cell growth. Nucleotide sequences of several promoters coincided with known sigma 43, sigma 29, sigma 28-promoters. Nucleotide sequences of other promoters differ greatly from those used by the known forms of Bacillus subtilis RNA polymerase. The existence of at least four other forms of bacilli RNA polymerase specific for these promoters is suggested.
D001412:Bacillus subtilis / Q000235:genetics*; D001435:Bacteriophages / Q000235:genetics*; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D010957:Plasmids; D011401:Promoter Regions, Genetic
D012321:DNA-Directed RNA Polymerases
Klonirovanie v kletkakh Bacillus subtilis i izuchenie osobennosteĭ promotornykh uchdstkov DNK Bacillus mesentericus i faga SPO2.