Modulation of CD4 antigen expression on human gingival Langerhans cells by gamma interferon.
Human gingival organ culture was used to test the hypothesis that gamma interferon (gamma-IFN), which induces HLA-DR and HLA-DQ antigens on Langerhans cells, modulates CD4 antigen expression on these same cells. Before culture, the density of CD4+ cells in the gingival epithelium was 12.7% +/- 3.8% of the density of CD1+ cells. gamma-IFN (200 IU/ml) enhanced CD4 expression such that at 24 h, all CD1+ cells also expressed CD4. Prostaglandin E2, which in isolation had no effect, partially abrogated the stimulatory effect of gamma-IFN on CD4 expression. These results imply that gamma-IFN and prostaglandin E2 are two signals which regulate CD4 expression on gingival Langerhans cells.
D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis*; D015232:Dinoprostone; D005881:Gingiva / Q000276:immunology*; D006684:HLA-DR Antigens / Q000032:analysis; D006801:Humans; D007124:Immunoenzyme Techniques; D007213:Indomethacin / Q000494:pharmacology; D007371:Interferon-gamma / Q000494:pharmacology*; D007801:Langerhans Cells / Q000276:immunology*; D009924:Organ Culture Techniques; D011458:Prostaglandins E / Q000494:pharmacology; D012867:Skin / Q000276:immunology; D013997:Time Factors
D000945:Antigens, Differentiation, T-Lymphocyte; D006684:HLA-DR Antigens; D011458:Prostaglandins E; D007371:Interferon-gamma; D015232:Dinoprostone; D007213:Indomethacin