Comparison of chondroitin sulfate to McCarey-Kaufman medium for corneal storage.
We compared endothelial preservation of paired corneas by scanning electron microscopy after storage in McCarey-Kaufman medium for two or three days to 2.5% chondroitin sulfate (K-Sol) for seven or ten days. One cornea from each of ten pairs of eyes was placed in McCarey-Kaufman medium and the opposite cornea was placed in K-Sol medium. We judged cellular preservation in a masked manner from the electron micrographs according to a morphologic grading system. We found no difference in endothelial structure between the two groups of corneas.
D002470:Cell Survival; D002809:Chondroitin Sulfates; D016039:Corneal Transplantation; D003470:Culture Media; D004728:Endothelium, Corneal / Q000648:ultrastructure; D006801:Humans; D008855:Microscopy, Electron, Scanning; D009926:Organ Preservation / Q000379:methods*
D003470:Culture Media; D002809:Chondroitin Sulfates