Immunoinhibition assay of the serum activity of human glycogen isophosphorylase BB in the diagnosis of the acute myocardial ischaemia.
Polyclonal rabbit anti-human isophosphorylases inhibit selectively the isoenzyme activities of the BB and MM isoenzyme of the human glycogen phosphorylase b with high sensitivity and without crossreactivities. On this basis a serological immunoinhibition assay of the BB isoenzyme in the range of 1-125 nmol s-1 l-1 is described. Preliminary clinical results indicate the BB isoenzyme as a sensitive and specific marker of the acute myocardial ischaemia including bypass surgery and suggest a substantial improvement of the differential diagnosis of infarction.
D003327:Coronary Disease / Q000175:diagnosis / Q000201:enzymology*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007527:Isoenzymes / Q000097:blood*; D009203:Myocardial Infarction / Q000175:diagnosis / Q000201:enzymology; D010763:Phosphorylase b / Q000097:blood*; D006005:Phosphorylases / Q000097:blood*; D012016:Reference Values
D007527:Isoenzymes; D010763:Phosphorylase b; D006005:Phosphorylases