title: Challenge of phagocyte metabolism by extracorporeal circulation and membrane contact. A biocompatibility test.
abstract: In the present study, direct data on phagocyte metabolism during hemodialysis are collected by the determination of 14CO2-production from labelled glucose on whole blood samples. Fifteen minutes after the start of cuprophan dialysis, 14CO2 production per 10(3) phagocytes increased more than four-fold (from 77.6 +/- 30.2 to 315.0 +/- 69.6 DPM/10(3) phagocytes; P less than 0.01). Such a change was absent during dialysis with membranes containing polysulphone, AN69S or reused cuprophan. In vitro contact of normal blood with cuprophan membranes revealed an increase of 14CO2-production by a maximum of 9.0 +/- 3.4 X 10(3) D.P.M. at 15 min. (P less than 0.05). Such an increase was absent after contact with polysulphone. The measurement of 14CO2 production during glucose metabolism, by phagocytes present in micro-amounts of whole blood, is a valuable test for membrane biocompatibility. Cuprophan dialysis induces a challenge of phagocytic activity that is absent for dialysers containing other membranes.
mesh_terms: D000180:Acrylic Resins; D000181:Acrylonitrile / Q000031:analogs & derivatives; D002245:Carbon Dioxide / Q000096:biosynthesis*; D002482:Cellulose / Q000031:analogs & derivatives; D005947:Glucose / Q000378:metabolism; D006801:Humans; D008422:Materials Testing; D008567:Membranes, Artificial; D010586:Phagocytes / Q000378:metabolism*; D011108:Polymers; D006435:Renal Dialysis; D013450:Sulfones
chemical_list: D000180:Acrylic Resins; D008567:Membranes, Artificial; D011108:Polymers; D013450:Sulfones; D002245:Carbon Dioxide; C017662:polysulfone P 1700; C016892:AN-69; D002482:Cellulose; C009865:cuprammonium cellulose; D005947:Glucose; D000181:Acrylonitrile