Inhibition of galactosyltransferase activity by cacodylate buffer.
Cacodylate buffer, frequently used in the assay of galactosyltransferase, causes inhibition of enzyme purified from human pleural effusion. The inhibition is reflected by an increase in the Km (UDP-galactose) and a decrease in Vmax and cannot be reversed by the addition of 2-mercaptoethanol. It is suggested that alternative buffers be used for the study of the enzyme.
D001152:Arsenicals / Q000494:pharmacology*; D002021:Buffers; D002101:Cacodylic Acid / Q000494:pharmacology*; D005700:Galactosyltransferases / Q000037:antagonists & inhibitors*; D006801:Humans; D010996:Pleural Effusion / Q000201:enzymology
D001152:Arsenicals; D002021:Buffers; D002101:Cacodylic Acid; D005700:Galactosyltransferases