Peripheral blood adherent cells from AIDS patients inhibit normal T-colony growth through decreased expression of interleukin 2-receptors and production of interleukin 2.
Adherent cells display an important accessory role on normal T-cell colony formation. Since the in-vitro proliferation of T colony-forming cells (T-CFC) from AIDS patients is extremely impaired we studied the effect of patients' adherent cells on T-CFC growth. Patients' peripheral blood mononuclear cells (PBMC) were fractionated on the basis of rosette formation with sheep red blood cells and complement-mediated cytotoxicity with OKT3 monoclonal antibody (E-T3-). Both mature (E+) T cells and E-OKT3- cell fractions failed to generate T-cell colonies although colony growth could be obtained from unfractionated PBMC. In five out of 12 AIDS patients, adherent cell-depletion of PBMC enhanced the plating efficiency. Moreover, patients' but not normal adherent cells could inhibit normal T-cell colony growth in a dose-dependent manner. Media conditioned by patients' unstimulated adherent cells (LCM-A+p) also inhibit normal T-cell colony formation. In addition, LCM-A+p were capable of inhibiting interleukin 2-receptor (IL2-R) expression and interleukin 2 (IL2) production by normal mitogen-stimulated T cells. These LCM-A+p did not contain detectable reverse transcriptase activity nor could they infect the CEM T-cell line which is permissive to human immunodeficiency virus (HIV). Conversely, this adherent cell-derived inhibitory activity could be abrogated by heating or treatment with proteolytic enzymes. These findings indicate that the low T-cell colony formation in some AIDS patients could be due to adherent cell-derived inhibitory activity(ies).
D000163:Acquired Immunodeficiency Syndrome / Q000097:blood* / Q000378:metabolism; D001773:Blood Cells / Q000166:cytology* / Q000378:metabolism; D002448:Cell Adhesion; D003470:Culture Media; D006128:Growth / Q000187:drug effects; D006801:Humans; D007376:Interleukin-2 / Q000096:biosynthesis*; D008297:Male; D008934:Mitogens / Q000494:pharmacology; D009000:Monocytes / Q000378:metabolism / Q000502:physiology; D011971:Receptors, Immunologic / Q000502:physiology*; D015375:Receptors, Interleukin-2; D013234:Stem Cells / Q000473:pathology*; D013601:T-Lymphocytes / Q000473:pathology*; D014410:Tumor Stem Cell Assay
D003470:Culture Media; D007376:Interleukin-2; D008934:Mitogens; D011971:Receptors, Immunologic; D015375:Receptors, Interleukin-2