title: [Comparative study of purine nucleoside phosphorylase from rabbit kidney, spleen, liver and embryos].
abstract: Homogeneous preparations of purine nucleoside phosphorylase (EC 2.4.2.1) from rabbit kidney, spleen, liver and embryos were studied. The enzyme preparations do not differ in electrophoretic mobility. The molecular weight of the enzyme obtained from various sources was determined by gel filtration on Sephadex G-150 superfine and is about 90-92 kD. The enzyme subunits are identical in terms of molecular weight, as can be evidenced from sodium dodecyl sulfate polyacrylamide gel electrophoresis (Mr approximately 31 kD). The pH optima of these enzyme preparations for guanosine and xanthosine phosphorolysis are 6.2 and 5.7, respectively. The isoelectric point of purine nucleoside phosphorylase from rabbit kidney was determined in the presence of 9 M urea and is equal to 5.55. The enzyme is the most stable at pH 7.7; it is specific towards hypoxanthine and guanine nucleosides as well as towards xanthosine, but does not cleave adenine nucleosides. The Km values for guanosine and inosine are 1.4.10(-4) M and 1.2.10(-4) M, respectively. The enzyme does not catalyze the ribosyl transfer reaction in the absence of Pi.
mesh_terms: D000818:Animals; D002384:Catalysis; D004591:Electrophoresis, Polyacrylamide Gel; D004622:Embryo, Mammalian / Q000201:enzymology*; D004795:Enzyme Stability; D007668:Kidney / Q000201:enzymology*; D007700:Kinetics; D009928:Organ Specificity; D010430:Pentosyltransferases / Q000378:metabolism*; D011683:Purine-Nucleoside Phosphorylase / Q000302:isolation & purification / Q000378:metabolism*; D011817:Rabbits; D013154:Spleen / Q000201:enzymology*; D013379:Substrate Specificity
chemical_list: D010430:Pentosyltransferases; D011683:Purine-Nucleoside Phosphorylase
vernacular_title: Sravnitel'noe izuchenie purinnukleozidfosforilazy iz pochek, selezenki, pecheni i émbrionov krolika.