Co-oxidation of arachidonic acid and methimazole by prostaglandin endoperoxide synthetase.
The thiocarbamide methimazole stimulated O2 utilisation when added to reaction mixtures containing ram seminal vesicle microsomes (prostaglandin endoperoxide synthetase preparation) and arachidonic acid. This stimulation increased with increasing methimazole concentrations, as did the production of prostaglandin E. 2-[14C]Methimazole was co-oxidized to 1-methyl-2-[14C]imidazole.
D000818:Animals; D001095:Arachidonic Acids / Q000378:metabolism*; D008297:Male; D008713:Methimazole / Q000378:metabolism* / Q000494:pharmacology; D008861:Microsomes / Q000187:drug effects / Q000378:metabolism; D010084:Oxidation-Reduction; D010101:Oxygen Consumption / Q000187:drug effects; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism*; D011458:Prostaglandins E / Q000096:biosynthesis; D012669:Seminal Vesicles / Q000201:enzymology*; D012756:Sheep / Q000378:metabolism
D001095:Arachidonic Acids; D011458:Prostaglandins E; D008713:Methimazole; D011451:Prostaglandin-Endoperoxide Synthases