title: Immunocytochemical localization of neurofilament protein subunits in the spiral ganglion of the adult rat.
abstract: Spiral ganglion neurons from adult rats were treated with several monoclonal antibodies that react with neurofilaments (NFs) and NF subunits. An antibody against NFs used with immunocytochemical techniques showed a strong reaction with most neuron processes in the spiral ganglion, whereas only a few neurons presented a reaction. Using monoclonal antibodies against the 3 subunits, we obtained the same results with a small percentage of neurons labelled. From quantitative observations, reacting neurons showed the same percentage as and a smaller size than T II neurons observed with a more conventional method. This shows that reacting neurons are indeed T II neurons and that they can easily be differentiated by an accumulation of NFs in their perikaryon by well characterized commercially available antibodies.
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D002452:Cell Count; D003056:Cochlear Nerve / Q000032:analysis* / Q000166:cytology; D007150:Immunohistochemistry; D007381:Intermediate Filament Proteins / Q000032:analysis*; D008970:Molecular Weight; D016900:Neurofilament Proteins; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D000911:Antibodies, Monoclonal; D007381:Intermediate Filament Proteins; D016900:Neurofilament Proteins