Culture-amplified immunological detection of Mycoplasma pneumoniae in clinical specimens.
We have employed a monoclonal antibody directed to a Mycoplasma pneumoniae membrane-associated protein antigen in a culture-amplified detection system. Eleven culture-positive sputum specimens were detected significantly more early by the immunoassay. Specimens culture-positive for other probable mycoplasma spp. were negative by this technique.
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial / Q000032:analysis*; D003470:Culture Media; D006801:Humans; D015151:Immunoblotting; D009177:Mycoplasma pneumoniae / Q000276:immunology / Q000302:isolation & purification*; D013048:Specimen Handling; D013183:Sputum / Q000382:microbiology*
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D003470:Culture Media