A transplantable cell line derived from spontaneous hepatocellular carcinoma of the hereditary hepatitis LEC rat.
A serially transplantable rat hepatocellular carcinoma (HCC) line was established. The primary spontaneous HCC which developed in a 506-day-old male hereditary hepatitis LEC rat was inoculated into young LEC rats. Only this HCC of 18 primary HCCs was successful in serial transplantation. The established cell line was histologically identical to the primary HCC showing a well-differentiated type with a trabecular structure of tumorous hepatocytes. The characteristic of albumin production was maintained. Chromosome analysis revealed rather widely dispersed polyploid chromosome numbers with a modal value at 96. Every metaphase contained two to five unusually large marker chromosomes.
D000818:Animals; D005260:Female; D005784:Gene Amplification; D006520:Hepatitis, Animal / Q000150:complications*; D008114:Liver Neoplasms, Experimental / Q000209:etiology* / Q000235:genetics / Q000473:pathology; D008297:Male; D009368:Neoplasm Transplantation; D011519:Proto-Oncogenes; D051381:Rats; D014178:Translocation, Genetic; D014407:Tumor Cells, Cultured