[Isolation and characteristics of antibodies against synthetic fragments of oncoproteins. II. Antiserum against the transforming protein pp60src of Rous sarcoma virus].
To generate the antibodies to the transforming protein of Rous sarcoma virus (RSV) pp60src, rabbits were immunized with the peptide, corresponding to 415-421 sequence of pp60src. These antibodies immunoprecipitate pp60src in RSV-transformed chicken and mammalian cells, and also some proteins (45, 85 and 120 kDa), which could be autophosphorylated in vitro. It was shown that 415-421 sequence of pp60src is not recognized by the antibodies to pp60src from RSV-induced tumour bearing rabbits (TBR serum). In contrast to TBR serum, antibodies, generated against synthetic peptide, corresponding 415-421 sequence of pp60src couldn't be phosphorylated in vitro, when [gamma-32P]ATP is added to the immune complex. The antipeptide antibodies, bound to pp60src did not block phosphorylation of TBR immunoglobulins, added to this immune complex. Hence, 415-421 sequence of pp60src RSV containing the major tyrosine phosphorylation site does not take part in the kinase reaction in vitro.
D000818:Animals; D002472:Cell Transformation, Viral; D002645:Chickens; D007106:Immune Sera / Q000302:isolation & purification*; D015151:Immunoblotting; D015688:Oncogene Protein pp60(v-src); D010446:Peptide Fragments / Q000138:chemical synthesis / Q000276:immunology*; D011494:Protein Kinases / Q000378:metabolism*; D011817:Rabbits; D051381:Rats; D012191:Retroviridae Proteins / Q000235:genetics / Q000276:immunology*
D007106:Immune Sera; D010446:Peptide Fragments; D012191:Retroviridae Proteins; D011494:Protein Kinases; D015688:Oncogene Protein pp60(v-src)
Poluchenie i kharakteristika antitel k sinteticheskim fragmentam onkobelkov. II. Antisyvorotka k transformiruiushchemu belku virusa sarkomy kur Rausa pp60src.