A comparison of 32P-postlabelling and immunological methods to examine human lung DNA for benzo[a]pyrene adducts.
Human lung DNA isolated from surgical specimens has been examined for the presence of polycyclic aromatic hydrocarbon-DNA adducts using both 32P-postlabelling and immunological methods. Of 12 samples examined to date, five had detectable amounts of benzo[a]pyrene diol epoxide-DNA adducts (BPDE-DNA) as determined by the enzyme-linked immunosorbent assay (ELISA), after immunoaffinity concentration. Values ranged from 3.5 to 11.5 fmol/mg DNA. When the same group of samples was analysed using the 32P-postlabelling technique, adducts could be detected in all the samples examined. There was generally not a good correspondence between the two methods. The number of adducts measured by 32P-postlabelling ranged from 1-100 per 10(8) nucleotides, which is some two orders of magnitude higher than with the immunological method, indicating that the BPDE-DNA adduct is probably not the major adduct present in these samples.
D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide / Q000032:analysis* / Q000276:immunology; D004247:DNA / Q000032:analysis* / Q000276:immunology; D018736:DNA Adducts; D004101:Dihydroxydihydrobenzopyrenes / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008168:Lung / Q000032:analysis*; D010761:Phosphorus Radioisotopes
D018736:DNA Adducts; D004101:Dihydroxydihydrobenzopyrenes; D010761:Phosphorus Radioisotopes; C043931:benzo(a)pyrene-7,8-dihydrodiol-9,10-epoxide-DNA; D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide; D004247:DNA