Induction of ligand carrying "natural liposomes" in cancer cells.
N-ethyl-maleimide (NEM) induced plasma membrane vesiculation of human cancer cell line EJ was studied in vitro using immunoelectromicroscopic methods. We have demonstrated the presence of plasma membrane glycoconjugates and antigens on these vesicles. The effect of NEM or Ricin-antiRicin antibody complex internalisation was also followed. It was revealed that NEM is able to block the internalisation and stimulate the recycling arm of the complex-traffic. Multivesicular endosome-derived plasma membrane vesicles play import role in this recycling. According to the presented evidences it is possible to produce ligands carrying "natural liposomes".
D000936:Antigen-Antibody Complex; D000951:Antigens, Neoplasm / Q000032:analysis; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure*; D003593:Cytoplasm / Q000378:metabolism; D005033:Ethylmaleimide / Q000494:pharmacology*; D006001:Glycoconjugates / Q000378:metabolism; D006801:Humans; D007158:Immunologic Techniques; D008024:Ligands / Q000378:metabolism*; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D012276:Ricin / Q000276:immunology; D001749:Urinary Bladder Neoplasms / Q000648:ultrastructure*
D000936:Antigen-Antibody Complex; D000951:Antigens, Neoplasm; D006001:Glycoconjugates; D008024:Ligands; D012276:Ricin; D005033:Ethylmaleimide