Use of monoclonal antibodies to protein F of Pseudomonas aeruginosa as opsonins for phagocytosis by macrophages.
Five protein F-specific monoclonal antibodies were found to opsonize Pseudomonas aeruginosa for complement-independent phagocytosis by unelicited mouse peritoneal macrophages, mouse macrophage cell line P388D1, and human monocyte-derived macrophages. Immunoglobulin G1 antibodies seemed to be a preferred isotype.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001426:Bacterial Proteins / Q000276:immunology*; D002460:Cell Line; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D007700:Kinetics; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009000:Monocytes / Q000276:immunology; D009895:Opsonin Proteins / Q000276:immunology*; D010587:Phagocytosis; D011550:Pseudomonas aeruginosa / Q000276:immunology*
D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins; D007074:Immunoglobulin G; D009895:Opsonin Proteins