[The sensitivity of a number of cell cultures to different strains of Rickettsia prowazekii].
The sensitivity of some cell cultures to different R. prowazekii strains (strain E with low pathogenicity, virulent strain Breinl, strains ERifRI and EVir) has been studied with a view to the selection of an adequate culture for growing these strains and the study of their biological properties. Experiments on titration in cells have revealed that 6- to 7-day primary and secondary irradiated quail fibroblasts and human amnion cells FL show the maximum sensitivity to all strains under study, comparable to that of chick embryos. The sensitivity of 6- to 7-day primary and secondary irradiated chick fibroblasts is faintly pronounced, and 24-hour chick and quail fibroblasts are still less sensitive. Cells FL have shown high sensitivity to strain E and mutant ERifRI in prolonged subculturing for 140 and 63 days (the term of observation) respectively after a single inoculation.
D000818:Animals; D002478:Cells, Cultured; D004352:Drug Resistance, Microbial; D006801:Humans; D009154:Mutation; D012283:Rickettsia prowazekii / Q000472:pathogenicity*; D012293:Rifampin / Q000037:antagonists & inhibitors; D012692:Serial Passage; D013997:Time Factors; D014774:Virulence
D012293:Rifampin
Chuvstvitel'nost' riada kletochnykh kul'tur k razlichnym shtammam Rickettsia prowazekii.