Successful transplantation of adult and fetal mouse pancreas islet cells using anti-class II antibodies.
Fetal pancreas islet tissue of mice is able to proliferate in cell culture. The insulin production after 14 days is sufficient to reverse diabetes mellitus. The immunogenicity is reduced by tissue culture. By using monoclonal antibodies it could be shown that the expression of class II antigens on the surface of the cells is lower after tissue culture than before. Together with complement, the positive cells could be lysed, and transplantation even in completely different mice stems was successful.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000627:therapeutic use; D000920:Antibody-Dependent Cell Cytotoxicity; D003921:Diabetes Mellitus, Experimental / Q000628:therapy*; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D016381:Islets of Langerhans Transplantation; D051379:Mice; D008815:Mice, Inbred Strains
D000911:Antibodies, Monoclonal; D000949:Histocompatibility Antigens Class II