Isolation of the acidic phospholipid phosphatidylglycerol from pulmonary surfactant by sorbent extraction chromatography.
Aminopropyl solid-phase columns can be used for phospholipid fractionation. A method has been developed to elute the acidic phospholipid phosphatidylglycerol (PG) separately, and it is applicable to both standard (phospho)lipid mixtures and pulmonary surfactants. The simplicity, rapidity, and high recovery make this method of isolating PG superior to other chromatographic procedures.
D000818:Animals; D002852:Chromatography, Ion Exchange; D000882:Haplorhini; D006863:Hydrogen-Ion Concentration; D009994:Osmolar Concentration; D010713:Phosphatidylcholines / Q000302:isolation & purification; D010715:Phosphatidylglycerols / Q000302:isolation & purification*; D010716:Phosphatidylinositols / Q000302:isolation & purification; D010718:Phosphatidylserines / Q000302:isolation & purification; D010743:Phospholipids / Q000302:isolation & purification*; D011663:Pulmonary Surfactants / Q000032:analysis*; D012756:Sheep
D010713:Phosphatidylcholines; D010715:Phosphatidylglycerols; D010716:Phosphatidylinositols; D010718:Phosphatidylserines; D010743:Phospholipids; D011663:Pulmonary Surfactants