Transcriptional activity of the genes for apoproteins A-I and E in neonatal rat liver.
The expression of the genes for apoproteins A-I and E (apoA-I, apoE) in fetal and neonatal rat livers was quantitated by measurement of steady-state levels of mRNA and by assays of relative transcriptional activity using the nuclear 'run-on' technique. From the last day of gestation (day -1) to day 5 after birth (day 5), for apoE there was a 2.2-fold increase in relative transcriptional activity and a 2.6-fold increase in steady-state mRNA. For apoA-I, from day -1 to 3 there was a 1.25-fold increase in both transcription and steady-state mRNA levels. We conclude that the increase in steady-state mRNA for the apoE gene which occurs during liver development in the rat is facilitated primarily by transcriptional control.
D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism; D016632:Apolipoprotein A-I; D001054:Apolipoproteins A / Q000235:genetics* / Q000378:metabolism; D001057:Apolipoproteins E / Q000235:genetics* / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D005786:Gene Expression Regulation; D007158:Immunologic Techniques; D008099:Liver / Q000254:growth & development / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D014158:Transcription, Genetic
D016632:Apolipoprotein A-I; D001054:Apolipoproteins A; D001057:Apolipoproteins E; D012333:RNA, Messenger