Synergistic generation of tumoricidal macrophages by muroctasin and interferon-gamma.
The ability of N2-[(N-acetylmuramoyl)-L-alanyl-D-isoglutaminyl]-N6-stearoyl-L-lysine (MDP-Lys(L18), muroctasin), a synthetic muramyl dipeptide derivative, to generate tumoricidal macrophages was investigated. MDP-Lys(L18) alone at the dose employed, 100 ng/ml, did not activate macrophages, and by combination with interferon-gamma (IFN-gamma), synergistically generated tumoricidal macrophages. Priming by IFN-gamma of macrophages enhanced endocytosis of MDP-Lys(L18) by the macrophages, which must be one of the mechanisms of the synergism. Thus, MDP-Lys(L18) is suggested to be a potent macrophage activator.
D000119:Acetylmuramyl-Alanyl-Isoglutamine / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D000276:Adjuvants, Immunologic / Q000378:metabolism / Q000494:pharmacology*; D000818:Animals; D004357:Drug Synergism; D007371:Interferon-gamma / Q000378:metabolism / Q000494:pharmacology*; D008262:Macrophage Activation / Q000187:drug effects*; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism
D000276:Adjuvants, Immunologic; D000119:Acetylmuramyl-Alanyl-Isoglutamine; D007371:Interferon-gamma; C036866:romurtide