Quantitation of acidosis in neonatal brain tissue using the 31P NMR resonance peak of phosphoethanolamine.
31P NMR brain spectra were obtained from piglets over a range of mild hypocarbia to severe hypercarbia (PaCO225 to 198 mm Hg). The chemical shifts of the phosphoethanolamine and inorganic phosphate were used to calculate intracellular brain pH (pHet and pHpi, respectively). Both pHpi and pHet underwent parallel significant decreases during hypercarbia, corresponding to 51 and 53% pHregulation, respectively. We conclude that the chemical shift of the phosphomonoester peak in vivo can be used to measure decreases in intracellular pH in neonatal brain.
D000138:Acidosis / Q000175:diagnosis*; D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000378:metabolism*; D002245:Carbon Dioxide / Q000502:physiology; D004983:Ethanolamines; D009682:Magnetic Resonance Spectroscopy; D010313:Partial Pressure; D010761:Phosphorus Radioisotopes; D013552:Swine
D004983:Ethanolamines; D010761:Phosphorus Radioisotopes; D002245:Carbon Dioxide; C005448:phosphorylethanolamine