The role of blood platelets in nucleoside metabolism: regulation of platelet thymidine phosphorylase.
Blood platelets are the smallest cellular elements in mammalian blood. Because of their small size, platelets have an unusually large surface area: volume ratio and are exquisitely sensitive to a multitude of physiological and environmental stimuli. Platelets lack nuclei, but most possess functional mitochondria and remain capable of both anaerobic and aerobic energy metabolism, for which they utilise a variety of substrates including many which are cytotoxic and genotoxic for other (nucleated) cells. Nucleic acid precursors are amongst the potentially genotoxic compounds for which platelets have an apparently insatiable appetite. In particular platelets actively scavenge adenine and adenosine, which they convert to nucleotides and use in energy metabolism, but they also rapidly phosphorylase thymidine and liberate thymine into the extracellular medium. In addition, platelets contain non-metabolisable membrane-bound pools of adenine nucleotides which they secrete in response to strong agonists. Taken together, these observations suggest that blood platelets play an important role in nucleic acid precursor metabolism. In the previous paper we have shown that most thymidine phosphorylase activity present in normal human blood resides in the cytoplasm of platelets. Here we demonstrate that this enzyme activity can be modulated in a dose-dependent fashion, not only by substances recognised as platelet agonists and antagonists, but also by some compounds which are considered to be toxic, mutagenic and/or carcinogenic. The data which we present provide additional support for our previous suggestion that platelets regulate thymidine homeostasis and further imply that this is the normal, physiological, platelet function. Preliminary results suggest that assays of blood platelet thymidine metabolism may provide data with a wide variety of applications.
D000438:Alcohols / Q000494:pharmacology; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000494:pharmacology; D001792:Blood Platelets / Q000187:drug effects / Q000502:physiology*; D003094:Collagen / Q000494:pharmacology; D003208:Concanavalin A / Q000494:pharmacology; D003902:Detergents; D005557:Formaldehyde / Q000494:pharmacology; D005643:Fucose / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D006706:Homeostasis; D006801:Humans; D066298:In Vitro Techniques; D008353:Mannitol / Q000378:metabolism; D009705:Nucleosides / Q000378:metabolism* / Q000494:pharmacology; D010430:Pentosyltransferases / Q000097:blood*; D013917:Thrombin / Q000494:pharmacology; D013936:Thymidine / Q000097:blood*; D013939:Thymidine Phosphorylase / Q000097:blood*; D014475:Uncoupling Agents / Q000494:pharmacology
D000438:Alcohols; D001095:Arachidonic Acids; D003902:Detergents; D009705:Nucleosides; D014475:Uncoupling Agents; D003208:Concanavalin A; D005557:Formaldehyde; D016718:Arachidonic Acid; D005643:Fucose; D008353:Mannitol; D003094:Collagen; D010430:Pentosyltransferases; D013939:Thymidine Phosphorylase; D013917:Thrombin; D005990:Glycerol; D013936:Thymidine