Multiple sclerosis: effects of activated T-lymphocyte-derived products on organ cultures of nervous tissue.
Supernatants from multiple sclerosis (MS) T-lymphocytes cause damage to both myelin and glial cells in cerebellar cultures assessed visually and by radiolabel release. Control T-lymphocytes, even after phytohaemagglutinin (PHA) stimulation, yielded supernatants which induced only slight damage, and at later times patients with other neurological diseases (OND) gave variable results. These differences suggest that MS T-lymphocytes are pre-activated in vivo to produce demyelinating factors while control T-lymphocytes are not pre-activated to the same extent. The visual evidence of activation of cerebellar macrophage-like cells was a common finding after MS T-lymphocyte supernatant treatment but there was no correlation with the severity of demyelination. There was a positive correlation between the percentage IL-2 receptor-bearing lymphocytes and the degree of supernatant-induced in vitro demyelination.
D001402:B-Lymphocytes / Q000032:analysis; D002457:Cell Extracts / Q000494:pharmacology*; D006801:Humans; D008264:Macrophages / Q000187:drug effects / Q000648:ultrastructure; D009103:Multiple Sclerosis / Q000378:metabolism / Q000473:pathology*; D009186:Myelin Sheath / Q000187:drug effects / Q000648:ultrastructure; D009417:Nerve Tissue / Q000187:drug effects* / Q000378:metabolism; D009457:Neuroglia / Q000187:drug effects / Q000648:ultrastructure; D009924:Organ Culture Techniques; D010835:Phytohemagglutinins / Q000494:pharmacology; D011971:Receptors, Immunologic / Q000378:metabolism; D015375:Receptors, Interleukin-2; D013462:Sulfur Radioisotopes / Q000378:metabolism; D013601:T-Lymphocytes / Q000032:analysis*; D014020:Tissue Extracts / Q000494:pharmacology*
D002457:Cell Extracts; D010835:Phytohemagglutinins; D011971:Receptors, Immunologic; D015375:Receptors, Interleukin-2; D013462:Sulfur Radioisotopes; D014020:Tissue Extracts