Rapid isolation of DNA from Actinomyces.
DNA could not be quickly extracted from members of the genus Actinomyces by the usual methods of lysis. Treatment of 7 different actinomyces cells with lysozyme and achromopeptidase, both 5 mg/g wet cells, for 2 h, followed by SDS (0.2%), proteinase K (5 mg/g wet cells) and EDTA (lmM) for 1 h, lysed the cells. The yield obtained in one day was 337 micrograms per 200 mg of bacterial cells. The treatment was also found to work effectively on strains belonging to Veillonella, Staphylococcus, Fusobacterium and Bifidobacterium genera.
D000190:Actinomyces / Q000235:genetics*; D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification*; D004492:Edetic Acid / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D019286:Endopeptidase K; D009113:Muramidase / Q000378:metabolism; D012697:Serine Endopeptidases / Q000378:metabolism; D012967:Sodium Dodecyl Sulfate / Q000378:metabolism
D004269:DNA, Bacterial; D012967:Sodium Dodecyl Sulfate; D004492:Edetic Acid; D009113:Muramidase; D012697:Serine Endopeptidases; C036813:lysyl endopeptidase; D019286:Endopeptidase K