title: Isolation of additional monoclonal antibodies directed against cell surface antigens of Myxococcus xanthus cells undergoing submerged development.
abstract: Thirteen additional monoclonal antibodies directed against cell surface antigens of Myxococcus xanthus cells undergoing submerged development were isolated and partially characterized. As measured by quantitative enzyme-linked immunosorbent assay, 10 of these antibodies recognized antigens common to both vegetatively growing cells and cells undergoing submerged development; 3 antibodies recognized antigens specific to developing cells. Five antigens were revealed as single bands on Western blots (immunoblots), and one produced multiple, diffuse bands characteristic of lipopolysaccharide.
mesh_terms: D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D000936:Antigen-Antibody Complex; D000954:Antigens, Surface / Q000032:analysis*; D015153:Blotting, Western; D007074:Immunoglobulin G / Q000302:isolation & purification; D007700:Kinetics; D009231:Myxococcales / Q000254:growth & development / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D000954:Antigens, Surface; D007074:Immunoglobulin G