title: Thiol-sensitive promoters of Escherichia coli.
abstract: Mu dX(lac) insertion mutants of Escherichia coli CSH50 in which the expression of the lacZ gene was sensitive to the presence of exogenous 1-thioglycerol or dithiothreitol were isolated. Both stimulatory and inhibitory mutants were found. The existence of several thiol-sensitive promoters suggests that exogenous thiols may provoke global stress responses in E. coli.
mesh_terms: D000693:Anaerobiosis; D004229:Dithiothreitol / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D005990:Glycerol / Q000031:analogs & derivatives / Q000494:pharmacology; D009154:Mutation; D011401:Promoter Regions, Genetic / Q000187:drug effects*; D013438:Sulfhydryl Compounds / Q000494:pharmacology*; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D013438:Sulfhydryl Compounds; C009465:thioglycerol; D001616:beta-Galactosidase; D005990:Glycerol; D004229:Dithiothreitol