title: Temporally specific involvement of cell surface beta-1,4 galactosyltransferase during mouse embryo morula compaction.
abstract: Cell surface beta-1,4 galactosyltransferase (GalTase) is shown to mediate intercellular adhesions between embryonal carcinoma (EC) cells and specifically during late morula compaction in the preimplantation mouse embryo. Monospecific anti-GalTase IgG raised against affinity-purified bovine beta-1,4 GalTase recognizes F9 EC cell GalTase as judged by immunoprecipitation and inhibition of GalTase activity, as well as by immunoprecipitation of a single 52 kd metabolically labeled membrane protein. Anti-GalTase IgG inhibits cell adhesions between EC cells, dissociates compacted mouse morulae, and inhibits blastocyst formation. Anti-GalTase IgG specifically inhibits cell adhesions during late morula compaction, coincident with a peak of surface GalTase activity as determined by direct enzyme assay. On EC cells, GalTase activity can be proteolytically released from intact cells, and is localized by indirect immunofluorescence to areas of intercellular contact, consistent with its proposed role in cell adhesion. Beta-1,4 GalTase is the first cell adhesion molecule identified that participates during late morula compaction, subsequent to uvomorulin function.
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002460:Cell Line; D002462:Cell Membrane / Q000201:enzymology / Q000648:ultrastructure; D002970:Cleavage Stage, Ovum / Q000166:cytology*; D005700:Galactosyltransferases / Q000502:physiology*; D007106:Immune Sera; D007074:Immunoglobulin G; D007700:Kinetics; D008565:Membrane Proteins / Q000502:physiology*; D051379:Mice; D009028:Morula / Q000166:cytology*; D009924:Organ Culture Techniques
chemical_list: D007106:Immune Sera; D007074:Immunoglobulin G; D008565:Membrane Proteins; D005700:Galactosyltransferases