title: S-100-immunoreactive nerves in the guinea-pig uterus with reference to ultrastructural correlations: effects of chemical sympathectomy and pregnancy.
abstract: In the guinea-pig uterus, by use of an indirect immunofluorescence method, S-100 immunoreactivity was found to be restricted to nerves that corresponded in number, distribution and type to adrenergic axons and pre-terminals. With advancing pregnancy S-100 immunoreactivity completely disappeared in uterine tissue adjacent to a fetus, in parallel with an ultrastructural degeneration of the adrenergic innervation. In the cervix and the uterine horn devoid of a fetus, however, the number and distribution of S-100-immunoreactive nerves was seemingly unchanged and no ultrastructural changes were found in adrenergic nerves. In contrast, chemical sympathectomy produced by 6-hydroxydopamine did not change S-100 immunoreactivity of uterine nerves. These findings suggest that there are differences in the denervation effected by chemical and by pregnancy-induced sympathectomy. The latter probably represents a special type of adrenergic denervation by inducing a degeneration of Schwann cells in addition to destruction of neuronal structures. This may explain the differences in the speed of regeneration of uterine adrenergic nerves following the two types of denervation.
mesh_terms: D000818:Animals; D001341:Autonomic Nervous System / Q000166:cytology* / Q000648:ultrastructure; D005260:Female; D005455:Fluorescent Antibody Technique; D006168:Guinea Pigs; D006892:Hydroxydopamines; D016627:Oxidopamine; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000502:physiology*; D009418:S100 Proteins / Q000032:analysis* / Q000276:immunology; D013562:Sympathectomy; D014599:Uterus / Q000294:innervation* / Q000648:ultrastructure
chemical_list: D006892:Hydroxydopamines; D009418:S100 Proteins; D016627:Oxidopamine