Immunological characterization of soluble peroxidases from rat tissues including preputial gland.
A highly active soluble peroxidase has been identified in the preputial gland of rats and characterized immunologically along with other soluble peroxidases of a number of rat tissues such as submaxillary gland, exorbital lacrimal gland and also of the uterine fluid of the estrogen treated rats. All these peroxidases have the native molecular weight around 73K as determined by gel filtration on Sephadex G-150. An antiserum raised against the pure bovine lactoperoxidase interacts with all these soluble peroxidases and immunoprecipitates the enzyme activity in a similar fashion when titrated against varied concentration of the antiserum. Following electrophoretic transfer to nitrocellulose by Western blotting, the antiserum crossreacts with the preputial, submaxillary and lacrimal gland protein of molecular weight around 73K and with the uterine fluid protein of molecular weight of 80K. An additional crossreacting protein of molecular weight of 80K is also evident in the lacrimal gland. All these enzyme preparations, however, contain another immunoreactive protein of molecular weight of about 64K. While 73-80K molecular weight interacting proteins may represent different forms of peroxidase, presumably with varied carbohydrate moieties, 64K molecular weight protein may be a precursor of the peroxidase which after posttranslational modification such as heme conjugation and glycosylation leads to formation of native enzyme. Rat harderian gland, unlike bovine origin, does not contain any detectable peroxidase activity.(ABSTRACT TRUNCATED AT 250 WORDS)
D000818:Animals; D000937:Antigen-Antibody Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D004958:Estradiol / Q000494:pharmacology; D005088:Exocrine Glands / Q000201:enzymology*; D005260:Female; D005779:Immunodiffusion; D007765:Lacrimal Apparatus / Q000201:enzymology; D007784:Lactoperoxidase / Q000276:immunology* / Q000378:metabolism; D008970:Molecular Weight; D010544:Peroxidases / Q000276:immunology*; D051381:Rats; D011919:Rats, Inbred Strains; D013045:Species Specificity; D013363:Submandibular Gland / Q000201:enzymology; D014599:Uterus / Q000187:drug effects / Q000201:enzymology
D004958:Estradiol; D007784:Lactoperoxidase; D010544:Peroxidases