Differential sulphation of chondroitins in murine T and B lymphocytes and lymphoma cells.
Splenic B and T lymphocytes metabolically labelled in vitro with [35S]-H2SO4 show differential sulphation of chondroitin sulphate, the major sulphated glycosaminoglycan in both cell-retained and secreted fractions. In B cells, 6-O-sulphation is more abundant than 4-O-sulphation, whereas in T cells sulphation occurs predominantly in the 4-O-position. The murine lymphoma lines AKTB-1b and EL-4, of B and T origin respectively, show even more marked differences. AKTB-1b follows the pattern of normal B cells, whilst in EL-4 only chondroitin 4-sulphate is synthesized.
D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism*; D002807:Chondroitin / Q000031:analogs & derivatives*; D002809:Chondroitin Sulfates / Q000096:biosynthesis*; D002851:Chromatography, High Pressure Liquid; D008223:Lymphoma / Q000378:metabolism*; D051379:Mice; D008806:Mice, Inbred AKR; D008809:Mice, Inbred C3H; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000378:metabolism*
D002807:Chondroitin; D002809:Chondroitin Sulfates