Human macrophage-activating factors for cytotoxicity. II. Synergism of two factors produced by a human T-cell hybridoma that induce the cytotoxicity of human monocytes.
Two different factors (MAF-C I and MAF-C II) were obtained by anion exchange chromatography of the culture supernatant of a human T-cell hybridoma, H3-E9-6, which produces macrophage-activating factors for cytotoxicity (MAF-C). These 2 factors induced the cytotoxicity of monocytes synergistically as a priming signal (MAF-C I) and a triggering signal (MAF-C II), respectively. On gel filtration on a column of Superose 12, MAF-C II was eluted mainly at the void volume, whereas MAF-C I was eluted in the fractions corresponding to approximate molecular weights of 30-300 K. On the other hand, gel filtration in the presence of sodium deoxycholate revealed that MAF-C II has an approximate molecular weight of 40,000, but MAF-C I was unstable under these conditions. When the activity for mouse macrophages (MAF-Cm activity) was tested, the MAF-C II fraction showed high MAF-Cm activity in the presence of murine recombinant interferon gamma (rIFN-gamma), but the MAF-C I fraction did not show MAF-Cm activity even in the presence of lipopolysaccharide (LPS). These results suggest that MAF-C I (priming lymphokine) has species specificity but MAF-C II (triggering lymphokine) does not.
D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003602:Cytotoxicity, Immunologic; D004357:Drug Synergism; D006801:Humans; D006825:Hybridomas; D007371:Interferon-gamma / Q000494:pharmacology; D008222:Lymphokines / Q000032:analysis / Q000494:pharmacology*; D008262:Macrophage Activation; D016215:Macrophage-Activating Factors; D008264:Macrophages / Q000276:immunology*; D009000:Monocytes / Q000276:immunology*; D011994:Recombinant Proteins / Q000494:pharmacology; D013045:Species Specificity; D014407:Tumor Cells, Cultured / Q000276:immunology
D008222:Lymphokines; D016215:Macrophage-Activating Factors; D011994:Recombinant Proteins; D007371:Interferon-gamma