title: Effect of ribosome-inactivating proteins on ribosomes from Tetrahymena pyriformis and Acanthamoeba castellanii.
abstract: The effect of ribosome-inactivating proteins type 1 (single-chain) and type 2 (two-chain, toxins) on polyphenylalanine polymerization by Tetrahymena pyriformis and Acanthamoeba castellanii ribosomes has been studied. The reaction catalysed by tetrahymena ribosomes was inhibited by two ribosome-inactivating proteins type 1 (dianthin 32 and, less effectively, momordin) whereas the reaction catalysed by amoeba ribosomes was inhibited, in a decreasing order of activity, by three ribosome-inactivating proteins type 1 (dianthin 32, saporin 6 and bryodin) and by two toxins (abrin and volkensin).
mesh_terms: D000048:Acanthamoeba / Q000187:drug effects / Q000235:genetics*; D000818:Animals; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D010452:Peptide Biosynthesis; D010455:Peptides; D014176:Protein Biosynthesis / Q000187:drug effects; D012270:Ribosomes / Q000187:drug effects / Q000378:metabolism*; D013769:Tetrahymena pyriformis / Q000187:drug effects / Q000235:genetics*; D014118:Toxins, Biological / Q000494:pharmacology*
chemical_list: D010455:Peptides; D014118:Toxins, Biological; C012001:polyphenylalanine; D008274:Magnesium