[Demonstration of glycocalyx formation in vitro in Citrobacter freundii, Proteus mirabilis and Planococcus sp.: the effect of polyosidases].
Three bacterial species of different origin, Citrobacter freundii, Proteus mirabilis and Planococcus sp., formed glycocalyx in vitro and thereby showed that the phenomena does not only occur in vivo contrary to the opinion of many authors. The glycocalyx was produced in the attachment processes used or both extra- and intercellular attachment. The aggregates formed caused large errors in bacterial counts and led to questioning of the very principle of the counting procedures. The glycocalyx was not destroyed by the polyosidases utilized nor by ultrasound treatment. The glycocalyx structure differed only according to the bacterial species of origin and was unchanged by culture media or nutrient deprivation.
D001422:Bacterial Adhesion; D002480:Cellulase / Q000378:metabolism; D002954:Citrobacter / Q000378:metabolism*; D003470:Culture Media; D005087:Glucan 1,4-alpha-Glucosidase / Q000378:metabolism; D006023:Glycoproteins / Q000096:biosynthesis*; D006026:Glycoside Hydrolases / Q000378:metabolism*; D008835:Micrococcaceae / Q000378:metabolism*; D008855:Microscopy, Electron, Scanning; D011096:Polygalacturonase / Q000378:metabolism; D011134:Polysaccharides / Q000096:biosynthesis*; D011513:Proteus mirabilis / Q000378:metabolism*; D014465:Ultrasonics; D000516:alpha-Amylases / Q000378:metabolism
D003470:Culture Media; D006023:Glycoproteins; D011134:Polysaccharides; D006026:Glycoside Hydrolases; D000516:alpha-Amylases; D011096:Polygalacturonase; D005087:Glucan 1,4-alpha-Glucosidase; D002480:Cellulase
Mise en évidence de la formation de glycocalyx in vitro chez Citrobacter freundii, Proteus mirabilis et Planococcus sp.: action de polyosidases.