Analysis and isolation of leukaemic and residual normal B lymphocyte populations from patients with chronic lymphocytic leukaemia.
Two colour fluorescent staining using anti-Leu-1 and anti-B1 monoclonal antibodies has been combined with continuous flow microfluorimetry to analyse and isolate the T and B cell populations in B cell chronic lymphocytic leukaemia (B-CLL) patients. In Rai stage 0 patients two distinct B1 positive populations were detected and sorted; one was Leu-1- and the other Leu-1+. Analysis of the isolated populations showed that the former (Leu-1- B1+) represented the residual normal B cells, whereas the latter represented monoclonal CLL B cells. Short term culture of these cells in vitro showed that the Leu-1+ B1+ population secreted IgM, but not IgG, following PMA stimulation.
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D001402:B-Lymphocytes / Q000145:classification / Q000276:immunology*; D002469:Cell Separation; D005434:Flow Cytometry; D006801:Humans; D007147:Immunoglobulin Light Chains / Q000032:analysis; D007945:Leukemia, Lymphoid / Q000276:immunology*; D007958:Leukocyte Count; D008214:Lymphocytes / Q000145:classification
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D007147:Immunoglobulin Light Chains