Transient activation of c-myc protooncogene expression in Leydig cells by human chorionic gonadotropin.
This study evaluated the effects of in vivo administration of human chorionic gonadotropin (hCG) on c-myc oncogene expression in Leydig cells. Sprague-Dawley rats (46-50 days old) were treated with hCG (10 units, i.p.), and purified Leydig cells were isolated 1-24 h later. HCG caused a transient elevation of c-myc mRNA in 4 h and returned to normal or lower than normal levels at 24 h. There was no change in c-fos or beta-actin mRNA levels. Our results suggest that the growth promoting effects of hCG on Leydig cells may be mediated by the transient expression of c-myc protooncogene.
D000199:Actins / Q000235:genetics; D000818:Animals; D015152:Blotting, Northern; D002455:Cell Division; D006063:Chorionic Gonadotropin / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D007985:Leydig Cells / Q000502:physiology*; D008297:Male; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D016760:Proto-Oncogene Proteins c-fos; D016271:Proto-Oncogene Proteins c-myc; D011519:Proto-Oncogenes; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
D000199:Actins; D006063:Chorionic Gonadotropin; D011518:Proto-Oncogene Proteins; D016760:Proto-Oncogene Proteins c-fos; D016271:Proto-Oncogene Proteins c-myc