[Solid phase immunoenzyme analysis of the immunospecificity of DNA from calf spleen alkylated by thiophosphamide].
DNA binding activity of rabbit antiserum against calf spleen DNA's modified by thiophosphamide (DNA-T) was studied by means of solid enzyme immunoassays (ELISA). The studies demonstrated the preferential binding of the immobilized DNA-T compared to immobilized single-stranded DNA (ss-DNA) and only small preference compared to native DNA. Two antisera against DNA-T were purified by affinity chromatography on a ss-DNA-CNBr agarose from antibodies to calf spleen ss-DNA. They interacted only with the immobilized DNA-T, but not with ss-DNA or native DNA. These results demonstrated that DNA modification by thiophosphamide, decreases the immunogenicity of usual nitrogen-containing DNA bases, but detected new immunogenic specificity for adducts. Detection of new immunogenic specificity in DNA's alkylated by thiophosphamide, resulted in the development of a sensitive enzyme immunoassay for the detection of these adducts in nucleic acids, in monitoring their formation, persistence and repair damages in DNA.
D000478:Alkylation; D000818:Animals; D002417:Cattle; D004247:DNA / Q000187:drug effects / Q000276:immunology*; D004277:DNA, Single-Stranded / Q000276:immunology; D007124:Immunoenzyme Techniques; D013154:Spleen; D013852:Thiotepa / Q000494:pharmacology*
D004277:DNA, Single-Stranded; D004247:DNA; D013852:Thiotepa
Tverdofaznyĭ immunofermentnyĭ analiz immunospetsifichnosti alkilirovannoĭ tiofosfamidom DNK selezenki telenka.