Commercial monoclonal antibodies for rapid detection of respiratory syncytial virus by direct immunofluorescence.
A commercially available direct immunofluorescence (IF) test using a reagent consisting of a pool of two monoclonal antibodies against selected surface and internal proteins of respiratory syncytial virus (RSV) was evaluated in comparison with the indirect IF technique using a commercial bovine anti-RSV hyperimmune serum for the rapid detection of RSV in nasopharyngeal aspirates from 228 hospitalized children. Overall agreement between the two IF methods was 95%. The direct IF test was quicker to perform and easier to interpret than the indirect IF test.
D000911:Antibodies, Monoclonal; D002648:Child; D005455:Fluorescent Antibody Technique; D006801:Humans; D009305:Nasopharynx / Q000382:microbiology; D011237:Predictive Value of Tests; D012136:Respiratory Syncytial Viruses / Q000302:isolation & purification*; D010253:Respirovirus Infections / Q000175:diagnosis*
D000911:Antibodies, Monoclonal