Isolation of type 5 capsular polysaccharide from Staphylococcus aureus.
Clinical surveys have indicated the predominance of type 5 and type 8 capsular polysaccharides among clinical isolates of Staphylococcus aureus. The type 5 capsular polysaccharide was extracted from three clinical isolates of S. aureus grown on solid media, and purified by ion exchange chromatography and gel filtration. Chemical analysis showed that the type 5 capsular polysaccharide is composed of N-acetylfucosamine and N-acetylhexosaminuronic acid. Immunodiffusion and 13C nuclear magnetic resonance experiments showed that the type 5 capsular polysaccharide is both immunologically and chemically distinct from the type 8 capsular polysaccharide and the teichoic acid of S. aureus.
D016667:Bacterial Capsules; D002849:Chromatography, Gas; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004590:Electrophoresis, Paper; D006801:Humans; D005779:Immunodiffusion; D009682:Magnetic Resonance Spectroscopy; D011135:Polysaccharides, Bacterial / Q000032:analysis / Q000302:isolation & purification*; D013211:Staphylococcus aureus
D011135:Polysaccharides, Bacterial; C054106:capsular polysaccharide, type 5, Staphylococcus aureus