Generation and isolation of cyanobacterial inside-out thylakoid vesicles.
A method has been designed to prepare inside-out thylakoid vesicles from a cyanobacterial species (Phormidium laminosum). Everted thylakoid vesicles could be generated by Yeda press treatment after induced membrane pairing. Membrane pairing was induced either by addition of high concentrations of Mg2+ ions or by lowering the pH of the fragmentation media. The inside-out vesicles were isolated by aqueous polymer two-phase partition. The membrane orientation was determined by proton translocation studies and freeze-fracture electron microscopy.
D002458:Cell Fractionation / Q000379:methods; D002734:Chlorophyll / Q000032:analysis; D000458:Cyanobacteria / Q000032:analysis* / Q000648:ultrastructure; D005614:Freeze Fracturing; D006863:Hydrogen-Ion Concentration; D007425:Intracellular Membranes / Q000032:analysis; D045342:Light-Harvesting Protein Complexes; D008274:Magnesium; D008854:Microscopy, Electron / Q000379:methods; D045322:Photosynthetic Reaction Center Complex Proteins; D010940:Plant Proteins / Q000032:analysis; D013347:Subcellular Fractions / Q000032:analysis*; D013499:Surface Properties
D045342:Light-Harvesting Protein Complexes; D045322:Photosynthetic Reaction Center Complex Proteins; D010940:Plant Proteins; D002734:Chlorophyll; D008274:Magnesium