Morphometric analysis of the cerebellar Purkinje cell in the developing normal and hypothyroid chick.
A morphometric analysis of Purkinje cells in the developing cerebellar cortex of the chick was performed in normal animals and embryos made hypothyroid by one or two spaced injections of tetramethylthiourea. Profiles of 162 Purkinje cells, from Golgi-Cox treated sections were analysed. Soma area, perimeter and circularity index, cumulative length of the dendrites and number of dendritic bifurcations were studied. The results showed significant differences between control and hypothyroid animals. There were no important differences between birds rendered transiently hypothyroid with a single injection and those made chronically hypothyroid with dual injections. This confirms that the Purkinje cell is very dependent on thyroid hormone especially during the early phases of its morphogenesis. The development of the Purkinje cell was the most affected process of cerebellar cortex maturation in the thyroid-deficient chick. The dendritic arborization was particularly hypoplastic. Moreover, a dynamic balance appeared to exist between the development of the dendritic arborization and that of the perikaryon.
D000818:Animals; D002452:Cell Count; D002525:Cerebellar Cortex / Q000196:embryology* / Q000254:growth & development / Q000503:physiopathology; D002642:Chick Embryo / Q000166:cytology* / Q000378:metabolism; D002645:Chickens / Q000254:growth & development* / Q000378:metabolism; D003712:Dendrites / Q000502:physiology; D007037:Hypothyroidism / Q000139:chemically induced / Q000196:embryology* / Q000503:physiopathology; D011689:Purkinje Cells / Q000166:cytology* / Q000502:physiology; D013890:Thiourea / Q000031:analogs & derivatives; D013963:Thyroid Hormones / Q000378:metabolism / Q000502:physiology*
D013963:Thyroid Hormones; D013890:Thiourea; C019107:tetramethylthiourea