title: Late-phase reaction in topically induced ocular anaphylaxis in the rat.
abstract: A cellular late-phase reaction is described in a rat model of topically induced ocular anaphylaxis. Rats were immunized with dinitrophenylated Ascaris suum extract and alum and were tested for active cutaneous anaphylaxis on day 13. Rats with a strong skin test response were selected for ocular challenge with di-DNP-lysine. Macroscopic observation and histologic evaluation were performed at 1, 6, and 24 h. In rats showing a moderate macroscopic ocular response at 1 h, mast cell degranulation was significantly increased at 1 h; no significant increase in eosinophils, neutrophils or lymphocytes was found in the conjunctive of these animals. In rats showing a marked macroscopic ocular response at 1 h, mast cell degranulation was significantly increased at 1 and 6 h; the number of eosinophils was significantly increased at 1 and 6 h, and of neutrophils at 6 h only. At 24 h, neutrophil and eosinophil numbers returned to baseline levels. There was no macroscopic evidence of a late-phase response in either group of animals. Our results suggest that, in keeping with earlier observations in human skin, a strong early response to antigen is required for the development of a late-phase ocular response in the rat.
mesh_terms: D000287:Administration, Topical; D000707:Anaphylaxis / Q000139:chemically induced / Q000473:pathology*; D000818:Animals; D001200:Ascaris / Q000276:immunology; D002452:Cell Count; D004487:Edema / Q000473:pathology; D004804:Eosinophils / Q000473:pathology; D005128:Eye Diseases / Q000139:chemically induced / Q000473:pathology*; D006968:Hypersensitivity, Delayed / Q000139:chemically induced / Q000473:pathology*; D007114:Immunization; D008239:Lysine / Q000031:analogs & derivatives; D008297:Male; D008407:Mast Cells / Q000473:pathology; D009504:Neutrophils / Q000473:pathology; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: C026230:bis(dinitrophenyl)lysine; D008239:Lysine