Suppression of footpad reaction in mice by 12-tetradecanoylphorbol 13-acetate and 7,12-dimethylbenz[a]anthracene.
The effect of treatment with 12-tetradecanoylphorbol 13-acetate (TPA), a tumor promoter, following 7,12-dimethylbenz[a]anthracene (DMBA), a tumor iniator, on foodpad reaction (FPR) with sheep blood red cells (SRBC) in mice was studied. After painting of 400 nmol of DMBA on the skins of the BALB/c mice immunized with SRBC, 8 nmol of TPA was painted every day for 7 days. Then, FPR was measured 24 hr after 2nd injection of SRBC. In these experiments, FPR was markedly suppressed and this effect continued for 2 weeks. When the spleen cells from the unimmunized donor mice which had been treated with DMBA and TPA were injected into the recipient mice was suppressed. These data show that the suppressive effect on FPR may be caused by the increase of the suppressor cells which are not specific for the antigen. This effect disappeared by the treatment of the spleen cells with Thy-1 or Lyt-2 antibody. These results suggest that the suppressor cells has Thy-1 and Lyt-2 antigens.
D015127:9,10-Dimethyl-1,2-benzanthracene / Q000494:pharmacology*; D000818:Animals; D004912:Erythrocytes / Q000276:immunology; D005528:Foot; D006968:Hypersensitivity, Delayed / Q000276:immunology / Q000517:prevention & control*; D007116:Immunization, Passive; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D012756:Sheep; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D015127:9,10-Dimethyl-1,2-benzanthracene; D013755:Tetradecanoylphorbol Acetate