Computer modeling of the neurotoxin binding site of acetylcholine receptor spanning residues 185 through 196.
A model of the complex between the acetylcholine receptor and the snake neurotoxin, cobratoxin, was built by molecular model building and energy optimization techniques. The experimentally identified functionally important residues of cobratoxin and the dodecapeptide corresponding to the residues 185-196 of acetylcholine receptor alpha subunit were used to build the model. Both cis and trans conformers of cyclic L-cystine portion of the dodecapeptide were examined. Binding residues independently identified on cobratoxin are shown to interact with the dodecapeptide AChR model.
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D003039:Cobra Neurotoxin Proteins / Q000378:metabolism*; D003198:Computer Simulation; D004546:Elapid Venoms / Q000378:metabolism*; D004557:Electric Organ; D008958:Models, Molecular; D011487:Protein Conformation; D011950:Receptors, Cholinergic / Q000378:metabolism*
D003039:Cobra Neurotoxin Proteins; D004546:Elapid Venoms; D011950:Receptors, Cholinergic