A cell-free analysis of the endocytic pathway.
The molecular control of the endocytic pathway is poorly understood. To obtain this information requires the use of cell-free systems which faithfully recreate the various endocytic events as they occur in the intact cell. Here I describe our approach to elucidating the mechanism which controls the fusion between different vesicles on the pathway.
D000818:Animals; D002459:Cell Fusion; D002474:Cell-Free System; D003034:Coated Pits, Cell-Membrane / Q000502:physiology; D004705:Endocytosis; D008722:Methods; D008954:Models, Biological; D013347:Subcellular Fractions