Antimutagens against spontaneous and induced reversion of a lacZ frameshift mutation in E. coli K-12 strain ND-160.
Isopropyl-beta-D-thiogalactoside (IPTG), at 0.5 or 1.0 mM, is shown to reduce spontaneous, and to virtually abolish caffeine- or quinacrine-induced reversions of the lacZ frameshift mutation in E. coli ND160. Guanosine at 200 micrograms/ml and Co2+ at 15 micrograms/ml had an erratic partial antimutagenic effect on spontaneous lac+ reversions. All 3 agents reduce caffeine-induced (500 micrograms/ml) mutagenesis. Spermine (250 micrograms/ml) also reduces quinacrine-induced Lac+ reversion frequencies in this system.
D002110:Caffeine / Q000037:antagonists & inhibitors; D003035:Cobalt / Q000494:pharmacology; D004926:Escherichia coli / Q000235:genetics*; D005696:Galactosidases / Q000235:genetics*; D006151:Guanosine / Q000494:pharmacology; D007544:Isopropyl Thiogalactoside / Q000494:pharmacology*; D009154:Mutation / Q000187:drug effects*; D011796:Quinacrine / Q000037:antagonists & inhibitors; D013096:Spermine / Q000494:pharmacology; D013865:Thioglycosides / Q000494:pharmacology*; D001616:beta-Galactosidase / Q000235:genetics*
D013865:Thioglycosides; D006151:Guanosine; D013096:Spermine; D007544:Isopropyl Thiogalactoside; D003035:Cobalt; D002110:Caffeine; D005696:Galactosidases; D001616:beta-Galactosidase; D011796:Quinacrine