title: Use of collagen corneal shields in the treatment of bacterial keratitis.
abstract: We used an animal model of Pseudomonas keratitis to compare treatment by topical tobramycin with and without the presence of a commercially available collagen corneal shield. Pilot studies showed a significant, 30-fold increase in penetration of tobramycin into the anterior chamber in eyes with a collagen shield in place. Twenty albino rabbit eyes were inoculated with P. aeruginosa to produce stromal keratitis. After 12 hours of topical tobramycin dosing, eyes with a collagen corneal shield in place had a statistically significant (P less than .01) decrease in colony forming unit counts in comparison to treated eyes without a shield and control eyes.
mesh_terms: D000818:Animals; D001082:Aqueous Humor / Q000378:metabolism; D001458:Bandages; D003094:Collagen / Q000627:therapeutic use*; D003114:Colony-Forming Units Assay; D005123:Eye / Q000382:microbiology; D007634:Keratitis / Q000209:etiology / Q000378:metabolism / Q000628:therapy*; D011552:Pseudomonas Infections; D011550:Pseudomonas aeruginosa / Q000302:isolation & purification; D011817:Rabbits; D014031:Tobramycin / Q000493:pharmacokinetics
chemical_list: D003094:Collagen; D014031:Tobramycin