title: RNA polymerase subunit homology among cyanobacteria, other eubacteria and archaebacteria.
abstract: RNA polymerase purified from vegetative cells of the cyanobacterium Anabaena sp. strain PCC 7120 contains a dissociable sigma factor and a core of five subunits: the beta', beta, and two alpha subunits characteristic of all eubacteria and an additional 66,000-molecular-weight polypeptide called gamma. Fifteen of fifteen strains of unicellular and filamentous cyanobacteria tested contained a serologically related gamma protein. Antiserum to gamma reacted with Escherichia coli beta' and the A subunit of RNA polymerase of the archaebacterium Sulfolobus acidocaldarius. Thus the evolution of the RNA polymerase beta' subunit has followed different paths in three groups of procaryotes: cyanobacteria, other eubacteria, and archaebacteria.
mesh_terms: D001105:Archaea / Q000201:enzymology* / Q000235:genetics / Q000276:immunology; D001419:Bacteria / Q000201:enzymology*; D005075:Biological Evolution; D003429:Cross Reactions; D000458:Cyanobacteria / Q000201:enzymology* / Q000235:genetics / Q000276:immunology; D012321:DNA-Directed RNA Polymerases / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics / Q000276:immunology; D007118:Immunoassay
chemical_list: D012321:DNA-Directed RNA Polymerases