title: Lactate dehydrogenase and malate dehydrogenase of Sertoli cells in rats.
abstract: Lactate-dehydrogenase (LDH) and malate-dehydrogenase (MDH) activities and isozyme patterns of maturing Sertoli cells were investigated in cultured cells obtained from 2-5-week-old rats. Both enzymes, and more strikingly LDH, increased between 2 and 3 weeks of age. LDH isozyme complement included all five isozymes and, in the more mature rats, had a shift toward the isoforms rich in subunit. These data are consistent with an increasing production of lactate/pyruvate and with a finer regulation of this production occurring during the maturing of Sertoli cells. Since lactate/pyruvate are important energy substrates for germ cells, the changes of LDH activity and isozyme patterns of maturing Sertoli cells support their nutritional role in the seminiferous epithelium.
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D007527:Isoenzymes / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D008291:Malate Dehydrogenase / Q000378:metabolism; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012708:Sertoli Cells / Q000201:enzymology*; D012741:Sexual Maturation
chemical_list: D007527:Isoenzymes; D005640:Follicle Stimulating Hormone; D007770:L-Lactate Dehydrogenase; D008291:Malate Dehydrogenase