Immunochemical detection of cytochrome P450C-M/F and NADPH-cytochrome P450 reductase in rat liver and kidney.
The localization and distribution of NADPH-cytochrome P450 reductase and cytochrome P450C-M/F were investigated immunohistochemically in the liver and the kidney of untreated rats employing both an unlabelled antibody peroxidase-antiperoxidase method and a peroxidase labelled primary antibody technique. In both immunohistochemical procedures, the reductase and P450C-M/F were detected in hepatocytes throughout the liver. In contrast, the reductase and P450C-M/F in the kidney were only detectable in the proximal tubule cells.
D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000032:analysis*; D007124:Immunoenzyme Techniques; D007668:Kidney / Q000201:enzymology*; D007687:Kidney Tubules, Proximal / Q000032:analysis; D008099:Liver / Q000201:enzymology*; D008297:Male; D009251:NADPH-Ferrihemoprotein Reductase / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D014018:Tissue Distribution
D003577:Cytochrome P-450 Enzyme System; D009251:NADPH-Ferrihemoprotein Reductase