title: Antibody profiles by EITB and ELISA of cattle and sheep infected with Fasciola hepatica.
abstract: In evaluating potential mechanisms of immunity in fascioliasis we compared the time-course analysis of the antibody responses between a resistant (cattle) and a susceptible model (sheep). Sera from sheep and cows experimentally infected with F. hepatica were reacted with both somatic (FhWWE) and excretory-secretory (ES) antigens in order to evaluate the antibody repertoires in the 2 different hosts. Analysis of these sera by ELISA showed a significant increase in antibody levels by 2 wk in most infected cattle using both somatic and ES antigens, whereas with most infected sheep antibodies are not clearly detected until week 4. By EITB, both infected sheep and cows recognize major somatic polypeptides in a molecular weight range of 30-38 kDa by 8 wk. Cattle recognized 3 additional major antigens of 56, 64, and 69 kDa as early as 6 wk. Various polypeptides of 20-25 kDa are prominently detected by most sheep and very faintly, if at all, by the cow sera. The sera from both sheep and cows also identify ES polypeptides of 20-28 kDa. The patterns of polypeptides recognized by sheep infected with S. mansoni and challenged with F. hepatica in EITB are almost identical to those with a simple F. hepatica primary infection. No significant differences were detected in the antibody kinetics in ELISA between these 2 groups. Differences and similarities between these models could eventually help determine which antibodies may be predictive of resistance or susceptibility in fascioliasis.
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000032:analysis*; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology / Q000469:parasitology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005210:Fasciola hepatica; D005211:Fascioliasis / Q000276:immunology / Q000662:veterinary*; D007124:Immunoenzyme Techniques / Q000662:veterinary; D012555:Schistosomiasis mansoni / Q000276:immunology / Q000662:veterinary; D012756:Sheep; D012757:Sheep Diseases / Q000276:immunology / Q000469:parasitology*; D013997:Time Factors
chemical_list: D000909:Antibodies, Helminth