Establishment and characterization of a human lymphoma cell line (WSU-NHL) with 14;18 translocation.
The WSU-NHL cell line was established from a malignant pleural effusion occurring in a 46-yr-old female with nodular histiocytic (follicular, large cell) lymphoma. The established cells grow in clumps with a doubling time of 57 h. On light microscopy, cells exhibited primitive lymphoblastoid morphologic features with few cytoplasmic blebs. DNA histogram generated by flow cytometry indicated a hypodiploid stemline (0.93). Immunologic study revealed a mature B-cell phenotype with surface and cytoplasmic IgG lambda and reactivity with monoclonal antibodies to B-cell antigens (B1, B4, BL1, BL3, BL4, BL7, HLA-DR, CALLA and Leu-10). The cells were negative for T-cell and myeloid-monocyte antigens as well as Epstein-Barr virus nuclear antigen (EBNA). Cytogenetic analysis revealed 45,XX metaphases with complex abnormalities including t(14;18) (q32;q21). The phorbol ester, 12-O tetradecanoylphorbol 13 acetate (TPA) (1.6 x 10(-8) M) and interferon gamma (IFN-gamma) (500 U/ml) inhibited cell growth and induced differentiation to a more mature phenotype. The WSU-NHL cell line provides a continuous source of cells for molecular and immunologic studies of human lymphoma as well as the regulation of its growth and differentiation by biologic agents.
D000944:Antigens, Differentiation, B-Lymphocyte / Q000032:analysis; D000951:Antigens, Neoplasm / Q000032:analysis; D001402:B-Lymphocytes / Q000276:immunology / Q000473:pathology; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D002883:Chromosomes, Human, Pair 14 / Q000648:ultrastructure*; D002887:Chromosomes, Human, Pair 18 / Q000648:ultrastructure*; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D016403:Lymphoma, Large B-Cell, Diffuse / Q000235:genetics / Q000473:pathology*; D008875:Middle Aged; D010996:Pleural Effusion / Q000473:pathology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014178:Translocation, Genetic; D014407:Tumor Cells, Cultured / Q000648:ultrastructure
D000944:Antigens, Differentiation, B-Lymphocyte; D000951:Antigens, Neoplasm; D004273:DNA, Neoplasm; D007371:Interferon-gamma; D013755:Tetradecanoylphorbol Acetate