Immunocytochemical localization of neurofilament protein subunits in the spiral ganglion of the adult rat.
Spiral ganglion neurons from adult rats were treated with several monoclonal antibodies that react with neurofilaments (NFs) and NF subunits. An antibody against NFs used with immunocytochemical techniques showed a strong reaction with most neuron processes in the spiral ganglion, whereas only a few neurons presented a reaction. Using monoclonal antibodies against the 3 subunits, we obtained the same results with a small percentage of neurons labelled. From quantitative observations, reacting neurons showed the same percentage as and a smaller size than T II neurons observed with a more conventional method. This shows that reacting neurons are indeed T II neurons and that they can easily be differentiated by an accumulation of NFs in their perikaryon by well characterized commercially available antibodies.
D000818:Animals; D000911:Antibodies, Monoclonal; D002452:Cell Count; D003056:Cochlear Nerve / Q000032:analysis* / Q000166:cytology; D007150:Immunohistochemistry; D007381:Intermediate Filament Proteins / Q000032:analysis*; D008970:Molecular Weight; D016900:Neurofilament Proteins; D051381:Rats; D011919:Rats, Inbred Strains
D000911:Antibodies, Monoclonal; D007381:Intermediate Filament Proteins; D016900:Neurofilament Proteins