Comparison of DNA adducts in mouse and rat epidermis versus dermis after topical application of (+/-)r-7,t-8-dihydroxy-t-9,10-oxy-7,8,9,10-tetrahydrobenzo(a)pyrene and (+/-) benzo(a)pyrene-4,5-oxide.
Mice and rats were treated topically with the BP metabolites, anti-BPDE and BPO. Rat epidermal DNA was extensively modified by BPO, while mouse epidermal DNA was preferentially modified by anti-BPDE. Anti-BPDE-dGuo adducts were observed only in mouse dermal DNA. DNA adducts were absent from the rat dermis. This adduct formation could be the reason for the very different in vivo biological effects of BP metabolites in the two species.
D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide / Q000378:metabolism*; D000287:Administration, Topical; D000818:Animals; D001580:Benzopyrenes / Q000378:metabolism*; D004247:DNA / Q000378:metabolism*; D004101:Dihydroxydihydrobenzopyrenes / Q000378:metabolism*; D008297:Male; D051379:Mice; D051381:Rats; D011919:Rats, Inbred Strains; D012867:Skin / Q000378:metabolism*; D013045:Species Specificity
D001580:Benzopyrenes; D004101:Dihydroxydihydrobenzopyrenes; C006707:benzo(a)pyrene 4,5-epoxide; D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide; D004247:DNA