[Possibility of identifying tumor promoters by their inhibitory action on the intercellular exchange of lucifer yellow].
The effects of tumor promoter--12-0-tetradecanoylphorbol-13-acetate (TPA), mezerein, anthralin, Ca2+-ionophore A23187, butylated hydroxytoluene (BHT), DDT and phenobarbital--on cell-to-cell exchange of Lucifer Yellow were studied in cultures of SV40-transformed Djungarian hamster fibroblasts. TPA, mezerein, A23187, DDT and BHT strongly inhibited cell-to-cell exchange of Lucifer Yellow. Anthralin uncoupled cells in 3 out of 6 experiments. Phenobarbital, in contrast to other promoters, enhanced dye transfer. The effects of all the promoters tested were fully reversible. The potential use of Lucifer Yellow exchange inhibition as a test for the screening of tumor promoters is discussed.
D000818:Animals; D000875:Anthralin; D002084:Butylated Hydroxytoluene; D000001:Calcimycin; D002273:Carcinogens; D002460:Cell Line; D006224:Cricetinae; D003634:DDT; D004224:Diterpenes; D005456:Fluorescent Dyes; D066298:In Vitro Techniques; D007546:Isoquinolines; D010634:Phenobarbital; D013729:Terpenes; D013755:Tetradecanoylphorbol Acetate
D002273:Carcinogens; D004224:Diterpenes; D005456:Fluorescent Dyes; D007546:Isoquinolines; D013729:Terpenes; D002084:Butylated Hydroxytoluene; C011309:mezerein; D000001:Calcimycin; C017475:lucifer yellow; D003634:DDT; D013755:Tetradecanoylphorbol Acetate; D000875:Anthralin; D010634:Phenobarbital
Vozmozhnost' vyiavleniia opukholevykh promotorov po ikh ingibiruiushchemu deĭstviiu na mezhkletochnyĭ obmen liutsiferovym zheltym.