Detection of epoxide hydrolase in rat hepatoma cell lines and primary rat liver cells by immunoblotting.
Rat epoxide hydrolase (EH) (EC 3.3.2.3) is elevated in cells of premalignant liver lesions, and variable EH activity has been reported for hepatocellular carcinomas. To facilitate detection of altered EH levels in liver cells, an immunoblotting method was devised. Rabbit antiserum specific for rat EH was prepared and used to detect EH extracted from suspensions of normal liver cells and from hepatoma cell lines. Compared with normal liver cells, 3 rat hepatoma cell lines, 7777, HTC and 17X, showed virtually undetectable EH levels by immunoblotting. The immunoblotting results rule out the possibility that very low EH enzymatic activity in the hepatoma cells results from production of normal amounts of non-functional enzyme protein.
D000818:Animals; D004851:Epoxide Hydrolases / Q000276:immunology / Q000378:metabolism*; D005779:Immunodiffusion; D007163:Immunosorbent Techniques; D008099:Liver / Q000201:enzymology*; D008114:Liver Neoplasms, Experimental / Q000201:enzymology*; D051381:Rats; D011916:Rats, Inbred F344; D014407:Tumor Cells, Cultured / Q000201:enzymology*
D004851:Epoxide Hydrolases