title: Intestinal lactase and other disaccharidase activities of a suckling crabeater seal (Lobodon carcinophagus).
abstract: 1. The intestinal disaccharidase activities of a suckling crabeater seal were investigated. 2. Lactase, maltase, isomaltase and cellobiase activities were readily detected but trehalase and sucrase activities were absent. 3. The intestinal homogenates were separated into a soluble (S2) fraction and a particulate brush border (P2) fraction. The lactase activities of the two fractions had different properties corresponding to those of an acid and a neutral beta-galactosidase respectively. Approximately two-thirds of the total lactase activity measured at pH 6.0 was due to the acid beta-galactosidase. 4. The isomaltase and cellobiase activities were found almost exclusively in the particulate fractions but about one third of the maltase activity was in the S2 fraction. This soluble maltase activity appeared to be due to an acid maltase.
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D010872:Caniformia / Q000378:metabolism*; D004186:Disaccharidases / Q000378:metabolism*; D005696:Galactosidases / Q000378:metabolism*; D007422:Intestines / Q000201:enzymology*; D007700:Kinetics; D046048:Seals, Earless / Q000378:metabolism*; D001616:beta-Galactosidase / Q000378:metabolism*
chemical_list: D004186:Disaccharidases; D005696:Galactosidases; D001616:beta-Galactosidase