An ELISA technique for the measurement of C1q in cerebrospinal fluid.
Determination of the C1q content of cerebrospinal fluid (CSF) may be of value in understanding the immunological reactions occurring within the central nervous system (CNS). A double sandwich ELISA method has been developed for the detection of C1q in human serum and CSF. It uses polyclonal antibodies and is sensitive in the nanogram range. The mean concentrations of C1q were determined to be 127 micrograms/ml in serum and 0.4 microgram/ml in CSF. These results suggest that increased levels of C1q in the CSF play a role in some neurodegenerative disorders.
D000293:Adolescent; D000328:Adult; D000368:Aged; D000544:Alzheimer Disease / Q000097:blood / Q000134:cerebrospinal fluid; D000690:Amyotrophic Lateral Sclerosis / Q000097:blood / Q000134:cerebrospinal fluid; D003166:Complement Activating Enzymes / Q000032:analysis / Q000134:cerebrospinal fluid* / Q000592:standards; D003172:Complement C1 / Q000032:analysis / Q000134:cerebrospinal fluid* / Q000592:standards; D015922:Complement C1q; D004797:Enzyme-Linked Immunosorbent Assay / Q000592:standards; D006801:Humans; D005779:Immunodiffusion; D008875:Middle Aged; D012015:Reference Standards
D003172:Complement C1; D015922:Complement C1q; D003166:Complement Activating Enzymes