[Isolation and characteristics of plasmid DNA from Lactobacilli].
It was shown on a model of 2 collection strains of lactobacilli that their cultivation on a synthetic multicomponent medium with addition of threonine and subsequent exposure of the cells to lysozyme at 0 degrees C and to alkaline solution of sodium dodecylsulfate at 60 degrees C provided highly efficient detection of plasmid DNA in these organisms. Circular molecules of the plasmid DNA of the 7-kv length, their dimer forms and linear molecules were detected in both of the strains.
D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification* / Q000648:ultrastructure; D004587:Electrophoresis, Agar Gel; D005111:Extrachromosomal Inheritance; D007778:Lactobacillus / Q000235:genetics* / Q000254:growth & development / Q000648:ultrastructure; D007779:Lactobacillus acidophilus / Q000235:genetics / Q000254:growth & development / Q000648:ultrastructure; D008854:Microscopy, Electron; D010957:Plasmids
D004269:DNA, Bacterial
Vydelenie i nekotorye kharakteristiki plazmidnoĭ DNK iz laktobatsill.