Triamcinolone impairs the synthesis of collagen and noncollagen proteins in condylar cartilage of newborn mice.
The effects of triamcinolone hexacetonide (TH) on the synthesis of collagen and noncollagen proteins were tested in mandibular condylar cartilage of newborn mice. Four-day-old ICR mice received a single i.p. injection of TH at doses ranging from 0.4 to 4.0 mg/kg body weight. Hydrocortisone, deoxycorticosterone, dexamethasone, and progesterone were administered at a dose of 4.0 mg/kg. Test animals and nontreated and vehicle-treated controls were sacrificed after 24, 48, and 72 hours and were processed for electron microscopy. Additional animals were injected with 5 microCi of 3H-proline 2 hours before sacrifice. The specimens were extracted with 5% TCA containing 1 mM proline followed by 5% TCA, acetone, and ether, homogenized and digested with purified bacterial collagenase, and the amounts of radioactivity in collagenase digestible (CDP) and noncollagen proteins (NCP) were determined. The present results revealed that triamcinolone led to a significant dose-dependent decrease in the protein content of the tissue that lasted for 3 days (12-14% at the dose of 4 mg/kg). The incorporation of 3H-proline into CDP was reduced by 39, 57, and 42% at 24, 48, and 72 hours, respectively whereas the incorporation into NCP was reduced by 20, 35, and 23%, respectively. When compared with other steroids, dexamethasone revealed a similar inhibitory effect, whereas hydrocortisone and deoxycorticosterone had no significant effect. Progesterone, on the other hand, showed a transient (24 hours) stimulatory effect on the synthesis of collagen synthesis (21%, P less than 0.05). Electron microscopy showed an atypical arrangement of collagen fibers and accumulation of large aggregates of collagen that filled the entire matrical space between cartilage cells.
D000305:Adrenal Cortex Hormones / Q000494:pharmacology; D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism*; D002356:Cartilage / Q000187:drug effects* / Q000378:metabolism; D003094:Collagen / Q000096:biosynthesis*; D003900:Desoxycorticosterone / Q000494:pharmacology; D003907:Dexamethasone / Q000494:pharmacology; D006854:Hydrocortisone / Q000031:analogs & derivatives / Q000494:pharmacology; D008335:Mandibular Condyle; D051379:Mice; D008813:Mice, Inbred ICR; D011374:Progesterone / Q000494:pharmacology; D011392:Proline / Q000378:metabolism; D011500:Protein Synthesis Inhibitors / Q000378:metabolism; D014221:Triamcinolone / Q000494:pharmacology*; D014316:Tritium
D000305:Adrenal Cortex Hormones; D011500:Protein Synthesis Inhibitors; D014316:Tritium; D014221:Triamcinolone; D003900:Desoxycorticosterone; D011374:Progesterone; D003907:Dexamethasone; D003094:Collagen; D011392:Proline; D006854:Hydrocortisone