Differential expression of H-Y antigen on lymphocyte subsets: analysis by flow cytometry.
Expression of H-Y antigen in human white blood cells was measured using flow cytometry with monoclonal antibodies. In this system, lymphocytes were stained preferentially in the male, and to a lesser extent in the female. Analysis of the lymphocyte subsets with biotinylated H-Y antibody conjugated with streptavidin-fluorescein isothiocyanate (FITC) and subset-specific antibody conjugated with phycoerythrin derivative (RD1) revealed differential expression of H-Y among the subsets of the male. In samples from eight men, 41.1% +/- 21.7% of B cells (B1) were stained, compared with 20.7% +/- 12.8% of cytotoxic-suppressor T cells (T8) and 5.4% +/- 3.0% of helper-inducer T cells (T4). In samples from seven women, 12.4% +/- 10.9% of B cells were stained, but staining of T cells was negligible.
D000293:Adolescent; D000328:Adult; D000911:Antibodies, Monoclonal / Q000302:isolation & purification; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes; D006182:H-Y Antigen / Q000032:analysis*; D006801:Humans; D008214:Lymphocytes / Q000145:classification / Q000166:cytology / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D012016:Reference Values; D012737:Sex Factors; D013861:Thiocyanates
D000911:Antibodies, Monoclonal; D005452:Fluoresceins; D005456:Fluorescent Dyes; D006182:H-Y Antigen; D013861:Thiocyanates; D016650:Fluorescein-5-isothiocyanate