Effect of methoxy-p-benzoquinones and methoxy-p-hydroquinones on DNA synthesis in Ehrlich ascites tumor cells.
The effects of various methoxy-p-quinones and methoxy-p-hydroquinones on DNA synthesis and thymidine transport in Ehrlich Ascites Tumor Cells was investigated. The inhibitory concentrations (IC50) were in the range of 5 to 65 microM. Adriamycin, used as positive control, had an IC50 of 6 microM. 2,3-Dimethoxy-benzoquinone and -hydroquinone and 2,3,5,6-tetramethoxy-benzoquinone clearly inhibited DNA synthesis at concentrations where thymidine transport was not affected. 2-Methoxy-hydroquinone, 2-methoxy-benzoquinone, 2,6-dimethoxy-benzoquinone, 2,5-dimethoxy-benzoquinone had IC50-values close to the dose inhibiting also thymidine transport. 2,5-Dimethoxy-benzoquinone, 2,3,5-trimethoxy-hydroquinone, 2,3,5-trimethoxy-benzoquinone and 2,3,5,6-tetramethoxy-hydroquinone strongly inhibited thymidine transport without a significant effect on DNA synthesis. Addition of ascorbate enhanced the inhibitory activity of 2,6-dimethoxy-benzoquinone.
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism*; D004261:DNA Replication / Q000187:drug effects*; D007700:Kinetics; D051379:Mice; D011809:Quinones / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D013936:Thymidine / Q000378:metabolism
D011809:Quinones; D013936:Thymidine