Determination of the epinephrine-refractory hypoglycaemic activity of whole-cell pertussis vaccine in mice.
A new assay method has been developed for the quantitative estimation of the inhibitory effect of pertussis vaccine on epinephrine-induced hyperglycaemia in mice. The statistical analysis of the assay was based on logarithm-transformed estimates of the blood glucose levels. The method was sufficiently sensitive to detect the activity of 0.004 millilitre of commercial combined diphtheria-tetanus-whole cell pertussis vaccine. The estimated common variance was as small as 0.0034 and the assay was highly reproducible. Among commercial vaccines there was a significant difference in activity. The activity of a stock pertussis vaccine was inactivated by 5 mM glutaraldehyde at 37 degrees C for 30 min, but resisted treatment with 40 mM formaldehyde at 37 degrees C for 5 days. The extent of inactivation with the chemicals was calculated by a parallel line assay as the activity relative to that of untreated control pertussis vaccine.
D000818:Animals; D004168:Diphtheria Toxoid / Q000008:administration & dosage / Q000592:standards / Q000633:toxicity*; D015721:Diphtheria-Tetanus-Pertussis Vaccine; D004305:Dose-Response Relationship, Drug; D004338:Drug Combinations / Q000008:administration & dosage / Q000592:standards / Q000633:toxicity; D004837:Epinephrine / Q000494:pharmacology; D005260:Female; D005557:Formaldehyde / Q000494:pharmacology; D005976:Glutaral / Q000494:pharmacology; D007003:Hypoglycemia / Q000139:chemically induced*; D051379:Mice; D010567:Pertussis Vaccine / Q000008:administration & dosage / Q000592:standards / Q000633:toxicity*; D013745:Tetanus Toxoid / Q000008:administration & dosage / Q000592:standards / Q000633:toxicity*
D004168:Diphtheria Toxoid; D015721:Diphtheria-Tetanus-Pertussis Vaccine; D004338:Drug Combinations; D010567:Pertussis Vaccine; D013745:Tetanus Toxoid; D005557:Formaldehyde; D005976:Glutaral; D004837:Epinephrine