Differential expression of alpha-subunits of G-proteins in human neuroblastoma-derived cell clones.
The distribution of alpha- and beta-subunits of G-proteins was analyzed in membranes of three cell clones which are derived from the human neuroblastoma cell line SK-N-SH. The neuroblast-like clone SH-SY5Y shows a pattern of G-proteins very similar to that of human brain cortex with high levels of Gi alpha and Go alpha but low levels of G40 alpha. The intermediate clone SH-IN contains high levels of Go alpha and Gi alpha and moderate levels of G40 alpha. The non-neuronal clone SH-EP shows high levels of G40 alpha but lacks Go alpha. Differentiation of the neuroblast-like clone SH-SY5Y by retinoic acid or nerve growth factor does not change the amount of Gi alpha or Go alpha in the membrane.
D002454:Cell Differentiation / Q000187:drug effects; D002462:Cell Membrane / Q000032:analysis; D002999:Clone Cells / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000096:biosynthesis*; D006801:Humans; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D009414:Nerve Growth Factors / Q000494:pharmacology; D009447:Neuroblastoma / Q000473:pathology*; D014212:Tretinoin / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000378:metabolism*
D009363:Neoplasm Proteins; D009414:Nerve Growth Factors; D014212:Tretinoin; D019204:GTP-Binding Proteins