title: Identification and purification of a human immunoglobulin-enhancer-binding protein (NF-kappa B) that activates transcription from a human immunodeficiency virus type 1 promoter in vitro.
abstract: The enhancer-binding factor NF-kappa B, which is found only in cells that transcribe immunoglobulin light chain genes, has been purified from nuclear extracts of Namalwa cells (human Burkitt lymphoma cells) by sequence-specific DNA affinity chromatography. The purified NF-kappa B has been identified as a 51-kDa polypeptide by UV-crosslinking analysis. "Footprint" and methylation-interference analyses have shown that purified NF-kappa B has a binding activity specific for the kappa light chain enhancer sequence. The purified factor activated in vitro transcription of the human immunodeficiency virus type I promoter by binding to an upstream NF-kappa B-binding site.
mesh_terms: D001402:B-Lymphocytes / Q000378:metabolism*; D001483:Base Sequence; D002051:Burkitt Lymphoma / Q000473:pathology; D004268:DNA-Binding Proteins / Q000302:isolation & purification* / Q000378:metabolism; D004742:Enhancer Elements, Genetic; D005814:Genes, Viral; D006678:HIV / Q000235:genetics*; D006801:Humans; D007145:Immunoglobulin kappa-Chains / Q000235:genetics*; D008969:Molecular Sequence Data; D016328:NF-kappa B; D009363:Neoplasm Proteins / Q000302:isolation & purification / Q000378:metabolism; D009928:Organ Specificity; D011401:Promoter Regions, Genetic; D014157:Transcription Factors / Q000302:isolation & purification* / Q000378:metabolism; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured / Q000032:analysis
chemical_list: D004268:DNA-Binding Proteins; D007145:Immunoglobulin kappa-Chains; D016328:NF-kappa B; D009363:Neoplasm Proteins; D014157:Transcription Factors