[Methods of enhancing the specificity of immunoenzyme test systems for the determination of human IgE].
The sensitivity and specificity of an enzyme immunoassay (EIA) system for the determination of total IgE in humans increases if polystyrene plates are sensitized with swine gamma-globulin and the conjugate is prepared with the use of sheep gamma-globulin obtained from the commercial preparation of sheep antiserum to human IgE. Such system becomes even more specific and sensitive if sheep antibodies to human IgE, purified by affinity chromatography, are used both for the sensitization of polystyrene plates and for the preparation of the conjugate. This conjugate is necessary for the development of EIA systems intended for the determination of specific human IgE-antibodies to various allergens.
D000918:Antibody Specificity; D002846:Chromatography, Affinity / Q000379:methods; D006801:Humans; D005779:Immunodiffusion / Q000379:methods; D007122:Immunoelectrophoresis / Q000379:methods; D007124:Immunoenzyme Techniques / Q000295:instrumentation; D007073:Immunoglobulin E / Q000032:analysis*; D007163:Immunosorbent Techniques
D007073:Immunoglobulin E
Metody povysheniia spetsifichnosti immunofermentnykh test-sistem dlia opredeleniia IgE cheloveka.