Studies of ovulation in the perfused ovary of the fowl (Gallus domesticus).
A system was developed for the in-vitro perfusion of the fowl ovary. The ovaries were isolated 16-18 h before expected ovulation of the first follicle of a clutch sequence and perfused at 41 degrees C with Eagle's culture medium containing L-thyroxine and insulin. The efferent perfusion pressure was maintained at 30-40 mmHg. This model was used to investigate the mechanism of ovulation. Addition of LH (1 U) to the perfusate induced ovulation (46%) but LH (1 U) + FSH (1 U) was more effective (88%; P less than 0.05). Progesterone at 100 micrograms alone also induced ovulation (80%). Clomiphene prevented gonadotrophin-induced ovulation. These results suggest that progesterone may act directly on the ovary as a final hormone to induce ovulation in the domestic fowl.
D000818:Animals; D002645:Chickens / Q000502:physiology*; D002996:Clomiphene / Q000494:pharmacology; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D007986:Luteinizing Hormone / Q000494:pharmacology; D010053:Ovary / Q000187:drug effects / Q000502:physiology*; D010060:Ovulation / Q000187:drug effects; D010477:Perfusion / Q000295:instrumentation / Q000379:methods*; D011374:Progesterone / Q000494:pharmacology
D002996:Clomiphene; D011374:Progesterone; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone