Electron microscopy of abnormal secretory products of rat incisor ameloblasts in tetracycline-induced lesions.
Lesions were created in the incisor enamel organs of adult rats by a single dose of tetracycline, and the secretory products in these lesions were examined with the transmission electron microscope. The following abnormal products were found: 1) circumscribed areas of a mottled uncalcified material within the enamel; 2) an uncalcified material consisting of tubules with dimensions similar to the crystal coat of normal enamel, 3) spherical calcified masses which at their periphery resemble normal enamel; 4) layers of a basal lamina-like material in the extracellular spaces between Tomes' processes, and an attachment site-like modification of the cell borders next to this material. It is suggested a) that the enamel tubule is a primary secretory product of the ameloblast; in normal enamel it would support the growth of the crystal; b) that, in the lesion, enamel can grow by diffusion of precursors from distant ameloblasts, while rat enamel normally grows only in direct contact with the ameloblast cell membrane.
D000565:Ameloblasts / Q000187:drug effects / Q000648:ultrastructure*; D000818:Animals; D003743:Dental Enamel / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D004658:Enamel Organ / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D005260:Female; D007180:Incisor; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013752:Tetracycline / Q000009:adverse effects; D014083:Tooth Germ / Q000378:metabolism*
D013752:Tetracycline