title: The effect of deazaadenosine analogues on the proliferation rate of cultured Vero cells.
abstract: Five adenosine analogues resistant to adenosine deaminase were analyzed for their effects on the proliferation on cultured Vero cells. Cells were exposed for 48 hr to various concentrations of 1-deazaadenosine (c1Ado), 3-deazaadenosine (c3Ado), 7-deazaadenosine (c7Ado), 1,3-dideazaadenosine (c1,3Ado) or 1,7-dideazaadenosine (c1,7Ado) and then counted with a hemocytometer. Results indicate that 1) whereas c7Ado produced a marked decrease in cell growth, c3Ado was toxic only at the highest concentration tested (30 microM), 2) c1Ado and c1,7Ado did not affect cell replication and 3) c1,3Ado produced a significant stimulation of cell proliferation. Further studies have suggested that none of the compounds tested is incorporated in the DNA.
mesh_terms: D000818:Animals; D002455:Cell Division / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D004247:DNA / Q000096:biosynthesis; D000882:Haplorhini; D012263:Ribonucleosides / Q000494:pharmacology*; D014372:Tubercidin / Q000031:analogs & derivatives / Q000494:pharmacology*; D014709:Vero Cells
chemical_list: D012263:Ribonucleosides; D004247:DNA; D014372:Tubercidin