Porcine high molecular weight kininogen: its purification and properties as a thiol proteinase inhibitor as compared to human high molecular weight kininogen.
1. High mol. wt kininogen (HMW kininogen) was purified to a homogeneous state from porcine plasma. 2. The protein exhibited a strong inhibitory activity for thiol proteinases such as ficin, papain and calpain I, whereas it did not inhibit serine proteinases, trypsin and chymotrypsin. 3. The mol. wt, isoelectric point, amino acid and carbohydrate compositions, stabilities to temperature and pH, kinetic constants, and immunological properties of the porcine HMW kininogen were determined and compared with those of human HMW kininogen.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000937:Antigen-Antibody Reactions; D002241:Carbohydrates / Q000032:analysis; D002846:Chromatography, Affinity; D003429:Cross Reactions; D015853:Cysteine Proteinase Inhibitors; D006801:Humans; D006863:Hydrogen-Ion Concentration; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D007610:Kallikreins / Q000378:metabolism; D007704:Kininogens / Q000097:blood / Q000302:isolation & purification*; D008970:Molecular Weight; D013045:Species Specificity; D013552:Swine; D013816:Thermodynamics
D000596:Amino Acids; D002241:Carbohydrates; D015853:Cysteine Proteinase Inhibitors; D007704:Kininogens; D007610:Kallikreins