A rapid procedure for the isolation of phycobilisomes from cyanobacteria.
This paper describes a method for the rapid isolation of phycobilisomes using a cationic detergent, CTAB (cetyltrimethylammonium bromide). The method has distinct advantages over those currently in use in that (i) release of intact phycobilisomes from cells in the presence of CTAB occurs in 40 s (as compared to 40-60 min of incubation required with Triton X-100), thereby reducing the chances of proteolysis of the component phycobiliproteins; and (ii) these phycobilisome preparations have reduced chlorophyll contamination in the initial stages. In addition this method also helps retain the structural and functional properties, as evidenced by spectroscopy and sodium dodecyl sulfate-polyacrylamide gel analysis.
D002462:Cell Membrane / Q000032:analysis; D000077286:Cetrimonium; D002593:Cetrimonium Compounds; D000458:Cyanobacteria / Q000032:analysis*; D003902:Detergents; D004591:Electrophoresis, Polyacrylamide Gel; D006868:Hydrolysis; D045342:Light-Harvesting Protein Complexes; D017830:Octoxynol; D045524:Phycobilisomes; D010940:Plant Proteins / Q000302:isolation & purification*; D011092:Polyethylene Glycols; D013050:Spectrometry, Fluorescence
D002593:Cetrimonium Compounds; D003902:Detergents; D045342:Light-Harvesting Protein Complexes; D045524:Phycobilisomes; D010940:Plant Proteins; D011092:Polyethylene Glycols; D017830:Octoxynol; D000077286:Cetrimonium