Lysates of Sarcocystis cruzi bradyzoites stimulate RAW 264.7 macrophages to produce tumor necrosis factor (cachectin).
Lysate of Sarcocystis cruzi bradyzoites induced RAW 264.7 macrophages to release a factor that killed L 929 cells. Rabbit antibody to murine tumor necrosis factor (TNF) inhibited this killing effect, indicating that the factor causing cell death was similar to TNF (also called cachectin). Based on the present findings we hypothesize that TNF mediates, at least in part, the disease associated with this parasitic infection.
D000818:Animals; D002448:Cell Adhesion; D002460:Cell Line; D002470:Cell Survival; D003470:Culture Media; D008264:Macrophages / Q000378:metabolism*; D012522:Sarcocystis / Q000502:physiology*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
D003470:Culture Media; D014409:Tumor Necrosis Factor-alpha