Enzyme immunoassay for detection of monoclonal immunoglobulin in lymph nodes. A comparison with histologic and DNA gene rearrangement studies.
This article describes a 1-hour enzyme immunoassay (ELISA) for identifying lymph nodes in which a single immunoglobulin light chain isotype is abnormally predominant. Eighteen lymph nodes representing a variety of reactive hyperplasias and B-cell neoplasms were analyzed with the ELISA and submitted for B- and T-cell gene rearrangement studies at the CT beta, JH and J kappa (JK) loci. In all 18 specimens, there was agreement in the results of the ELISA, the histologic diagnoses, and the results of gene rearrangement studies. In three of the B-cell lymphomas identified by histologic, ELISA, and DNA studies, surface marker phenotyping by flow cytometry did not indicate the presence of a monoclonal cell population. It is concluded that the ELISA correlates well with DNA gene rearrangement studies and is a rapid, simple, and accurate method for identifying lymph nodes containing monoclonal immunoglobulin. Moreover, it is proposed that the ELISA has certain advantages over other methods for determining light chain clonality in clinical specimens, and it is therefore useful to distinguish between benign lymphoid hyperplasia and monoclonal B-cell neoplasms.
D004247:DNA / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D006801:Humans; D007147:Immunoglobulin Light Chains / Q000032:analysis*; D008198:Lymph Nodes / Q000276:immunology* / Q000473:pathology; D011995:Recombination, Genetic
D007147:Immunoglobulin Light Chains; D004247:DNA