Inhibition of DNA polymerase alpha by aphidicolin derivatives.
17-Acetylaphidicolin was 10-fold weaker and two derivatives lacking hydroxyl groups at the 16 and 17 positions were 100-fold weaker than aphidicolin as inhibitors of DNA polymerase alpha from HeLa and Chinese hamster ovary cells. 17,18-Diacetyl, 3,17,18-triacetyl and 3-epi derivatives of aphidicolin were inactive. Active compounds were, like aphidicolin, competitive with dCTP and did not inhibit aphidicolin-resistant DNA polymerases.
D000818:Animals; D016590:Aphidicolin; D006224:Cricetinae; D003412:Cricetulus; D004257:DNA Polymerase II / Q000037:antagonists & inhibitors*; D003845:Deoxycytosine Nucleotides / Q000378:metabolism; D004224:Diterpenes / Q000494:pharmacology*; D005260:Female; D005347:Fibroblasts / Q000201:enzymology; D006367:HeLa Cells / Q000201:enzymology; D010053:Ovary
D003845:Deoxycytosine Nucleotides; D004224:Diterpenes; C024107:2'-deoxycytidine 5'-triphosphate; D016590:Aphidicolin; D004257:DNA Polymerase II