Transfection of an immunoglobulin kappa gene into mature human B lymphocytes.
We show in this report that the transcription induced by interleukin-2 or pokeweed mitogens of the kappa MOPC 41 immunoglobulin light-chain gene transfected into primary human or murine B lymphocytes initiates from a previously unobserved start site about 26 base pairs upstream of the start site used in myeloma cell lines.
D000818:Animals; D001402:B-Lymphocytes / Q000502:physiology*; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D007145:Immunoglobulin kappa-Chains / Q000235:genetics*; D007371:Interferon-gamma / Q000494:pharmacology; D007376:Interleukin-2 / Q000494:pharmacology; D051379:Mice; D012333:RNA, Messenger / Q000235:genetics; D013601:T-Lymphocytes / Q000502:physiology; D014158:Transcription, Genetic / Q000187:drug effects; D014162:Transfection
D007145:Immunoglobulin kappa-Chains; D007376:Interleukin-2; D012333:RNA, Messenger; D007371:Interferon-gamma