Endothelial cell growth factor and ionophore A23187 stimulation of production of inositol phosphates in porcine aorta endothelial cells.
The existence of a bovine brain-derived endothelial cell growth factor has recently been reported, but its mode of action is unknown. We show that the endothelial cell growth factor is a potent stimulant of inositol monophosphate release in porcine aorta endothelial cells. Although the activation of phospholipase C by this factor does not appear to be dependent on Ca2+, the Ca2+ ionophore A23187 stimulates release of inositol phosphates. It is suggested that the inositol 1,4,5-trisphosphate 3-kinase/5-phosphomonoesterase pathway could account for the ionophore-induced changes in inositol 1,3,4-triphosphate.
D000818:Animals; D001011:Aorta; D000001:Calcimycin / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000378:metabolism*; D005346:Fibroblast Growth Factors / Q000494:pharmacology*; D007295:Inositol Phosphates / Q000096:biosynthesis*; D013268:Stimulation, Chemical; D013403:Sugar Phosphates / Q000096:biosynthesis*; D013552:Swine; D010738:Type C Phospholipases / Q000378:metabolism
D007295:Inositol Phosphates; D013403:Sugar Phosphates; D000001:Calcimycin; D005346:Fibroblast Growth Factors; D010738:Type C Phospholipases; D002118:Calcium