[Hybrid vectors for the cyanobacterium Anacystis nidulans R2, containing the plasmid pUB110 from Staphylococcus aureus].
The recombinant vector plasmids were constructed having the DNA of pUB110 plasmid (4,5 kb, KmR) from Staphylococcus aureus inserted into the cryptic plasmids pANS (8 Kb) and pANL (48,5 kb) of cyanobacterium Anacystis nidulans R2. The hybrid plasmids transform cyanobacterial cells to Km-resistance with high efficiency. The plasmid pBS20, containing the complete sequence of pANS and pUB110 DNA, transforms Bacillus subtilis rec E4 protoplasts being, however, unstable in bacilli cells and disintegrates deriving a parent pUB110 plasmid.
D002874:Chromosome Mapping; D000458:Cyanobacteria / Q000235:genetics*; D005822:Genetic Vectors; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D013211:Staphylococcus aureus / Q000235:genetics*; D014169:Transformation, Bacterial
Gibridnye vektory dlia tsianobakterii Anacystis nidulans R2, soderzhashchie plazmidu pUB110 iz Staphylococcus aureus.