A rapid method for the synthesis of protein-lipid complexes using adsorption chromatography.
A novel and rapid method for the detergent-mediated synthesis of protein-lipid complexes has been developed and has several advantages over detergent dialysis methods. This new method involves co-incubation of human apolipoprotein A-I (apoA-I), the major protein component of high density lipoproteins (HDL), and dipalmitoylphosphatidylcholine for 1 hr in the presence of cholate, after which removal of greater than 99.7% of the detergent is achieved by a 2-hr batch adsorptive chromatography procedure. Complexes prepared by this method had a density of 1.10 g/ml, similar to plasma HDL. Chemical cross-linking of these products demonstrated that there was complete conversion of apoA-I to a protein-lipid complex that contained two molecules of apoA-I. One major band was resolved by gradient gel electrophoresis in the region of the gel expected for newly synthesized HDL. Results are described which show the application of this method to the study of lipid variation on the structure of model HDL, including the alteration of lipid-protein molar ratios and the addition of cholesterol.
D015060:1,2-Dipalmitoylphosphatidylcholine; D016632:Apolipoprotein A-I; D001054:Apolipoproteins A; D002793:Cholic Acids; D002845:Chromatography / Q000379:methods; D008074:Lipoproteins / Q000138:chemical synthesis*
D016632:Apolipoprotein A-I; D001054:Apolipoproteins A; D002793:Cholic Acids; D008074:Lipoproteins; D015060:1,2-Dipalmitoylphosphatidylcholine