title: Modified in vivo platelet retention test for evaluating primary haemostasis.
abstract: We present a modified in vivo platelet retention test for evaluating platelet plug formation in a standardized incision applied to the skin (template bleeding time). In contrast to previously published similar procedures we determined platelet retention at defined time intervals. The investigation of 20 healthy subjects with this method revealed a characteristic biphasic pattern of platelet consumption at the wound surface, which could be correlated with primary and secondary platelet aggregation. In 10 patients with congenital platelet dysfunction and 2 patients with von Willebrand's disease the pattern of platelet retention was affected principally in its early phase, whereas aspirin, when given to 2 healthy subjects, inhibited platelet retention predominantly in its late phase. Our modified platelet retention test provides information about dynamic events associated with platelet plug formation in both normal and disordered primary haemostasis and may also be useful for evaluating in vivo effects of antithrombotic drugs.
mesh_terms: D000293:Adolescent; D000328:Adult; D001241:Aspirin / Q000008:administration & dosage; D001760:Bleeding Time; D001791:Blood Platelet Disorders / Q000097:blood; D005260:Female; D006487:Hemostasis / Q000187:drug effects; D006801:Humans; D008297:Male; D010974:Platelet Aggregation / Q000187:drug effects; D010976:Platelet Count / Q000187:drug effects; D010979:Platelet Function Tests; D014842:von Willebrand Diseases / Q000097:blood
chemical_list: D001241:Aspirin