The development of hemopoietic cells in response to stromal cells or growth factors is modified by agents that influence ADP-ribosylation.
The in vitro differentiation of multipotent stem cells in long-term marrow cultures can be blocked by treatment with agents that modify cholera toxin induced ADP-ribosylation of proteins. The latter agents also inhibit the growth and development of progenitor cells in soft gels in response to interleukin-3 but have little effect upon the development of progenitor cells that respond to the macrophage colony stimulating factor (CSF-1). Cholera toxin, in the same system, inhibits the development of CSF-1 responsive progenitor cells but has little effect on the development of cells that respond to IL-3. Similarly, progenitor cells that respond to IL-3 are relatively more resistant to pertussis toxin than cells that respond to CSF-1. These data indicate that ADP-ribosylation may be an important post-translational modification of regulatory proteins concerned with hemopoietic cell differentiation and growth in response to stromal cells or growth factors.
D000244:Adenosine Diphosphate / Q000378:metabolism*; D000818:Animals; D001853:Bone Marrow / Q000502:physiology*; D001854:Bone Marrow Cells; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D002772:Cholera Toxin / Q000494:pharmacology; D003115:Colony-Stimulating Factors / Q000494:pharmacology; D006133:Growth Substances / Q000494:pharmacology*; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000187:drug effects*; D037342:Pertussis Toxin; D012266:Ribose / Q000378:metabolism*; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
D003115:Colony-Stimulating Factors; D006133:Growth Substances; D010566:Virulence Factors, Bordetella; D000244:Adenosine Diphosphate; D012266:Ribose; D002772:Cholera Toxin; D037342:Pertussis Toxin