title: Chicken apolipoprotein A-I: cDNA sequence, tissue expression and evolution.
abstract: Using an antibody against chicken apolipoprotein (apo) A-I, we identified multiple cDNA clones for the protein in two intestinal cDNA libraries in lambda gt11. The complete nucleotide sequence of chicken apoA-I cDNA was determined. The sequence predicts a mature protein of 240 amino acids, a 6-amino acid propeptide and an 18-amino acid signal peptide. Using a 32P-cDNA probe, we detected the presence of apoA-I mRNA in 21 day old chicken intestine, liver, kidney, spleen, breast muscle and brain. The primary sequence of apoA-I contains numerous tandem repeats of 11 and 22 residues in a manner similar to the mammalian proteins. Our analysis of apoA-I sequences from human, rabbit, dog, rat, and chicken indicates that the rate of amino acid substitution is considerably faster in the rat lineage than in other mammalian lineages.
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D016632:Apolipoprotein A-I; D001054:Apolipoproteins A / Q000235:genetics*; D001483:Base Sequence; D005075:Biological Evolution; D002645:Chickens; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism; D005796:Genes; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism; D008969:Molecular Sequence Data; D009928:Organ Specificity; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D014158:Transcription, Genetic
chemical_list: D016632:Apolipoprotein A-I; D001054:Apolipoproteins A; D004247:DNA