[Polysaccharide-protein complexes isolated from different strains of Neisseria meningitidis serogroup B].
Fractionation of the biomass of 3 serogroup B N. meningitidis strains, obtained from solid serum-free and liquid synthetic media, by increasing concentrations of cetavlone revealed that the formation of natural polysaccharide-protein complexes with the ratio of their components approaching 1:1 was possible under the conditions ensuring the intensive synthesis of capsular polysaccharide. Two strains, 125 and 1642, grown on a solid amino peptide-containing medium regularly produced two polysaccharide-protein complexes with the protein/polysaccharide ratio approaching 1:1. One of these complexes passed easily into the supernatant fluid and could be precipitated with 0.1% cetavlone. The second complex was more firmly bound to the outer membrane of the cell and could be precipitated with 1% cetavlone. In most experiments an additional fraction with high protein content in relation to sialic acid was isolated from the biomass.
D001426:Bacterial Proteins / Q000302:isolation & purification*; D002458:Cell Fractionation / Q000379:methods; D003470:Culture Media; D006801:Humans; D019158:N-Acetylneuraminic Acid; D009345:Neisseria meningitidis / Q000145:classification / Q000254:growth & development / Q000302:isolation & purification*; D011135:Polysaccharides, Bacterial / Q000302:isolation & purification*; D012703:Serotyping; D012794:Sialic Acids / Q000302:isolation & purification
D001426:Bacterial Proteins; D003470:Culture Media; D011135:Polysaccharides, Bacterial; D012794:Sialic Acids; D019158:N-Acetylneuraminic Acid
Polisakharid-belkovye kompleksy, vydelennye iz raznykh shtammov Neisseria meningitidis serogruppy B.