Evaluation of striatal enkephalin release in vivo from steady state levels and turnover rates of the tripeptide Tyr-Gly-Gly.
The tripeptide Tyr-Gly-Gly (YGG), an extraneuronal metabolite of opioid peptides (OP) derived from proenkephalin A is in a highly dynamic state in mouse striatum. Inhibition of its synthesis by Thiorphan reduced its levels with a t 1/2 of 12 min. Inhibition of its degradation by bestatin elicited its rapid accumulation consistent with a t 1/2 of enkephalins in the one-hour range. Pentobarbital anesthesia markedly reduced its steady state level and turnover rates.
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D003342:Corpus Striatum / Q000187:drug effects / Q000378:metabolism*; D004745:Enkephalins / Q000378:metabolism*; D066298:In Vitro Techniques; D007700:Kinetics; D007930:Leucine / Q000031:analogs & derivatives / Q000494:pharmacology; D008297:Male; D051379:Mice; D009842:Oligopeptides / Q000096:biosynthesis / Q000378:metabolism*; D010424:Pentobarbital / Q000494:pharmacology; D015244:Thiorphan; D008625:Tiopronin / Q000031:analogs & derivatives / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D004745:Enkephalins; D009842:Oligopeptides; C044757:tyrosyl-glycyl-glycine; D015244:Thiorphan; D008625:Tiopronin; D007930:Leucine; C012211:ubenimex; D010424:Pentobarbital