Accumulation of c-fos mRNA in slices of mouse submandibular gland incubated in vitro.
Incubation of slices or isolated lobules of murine submandibular gland at 37 degrees C in physiologic solutions or in tissue culture media, or dissociation of the cells by collagenase-hyaluronidase treatment, increased the steady-state level of c-fos mRNA without any additional stimulus. This activation of c-fos expression required the presence of Na+ and K+ but not extracellular Ca2+. It was augmented by depolarizing concentrations of K+ and by veratridine, and inhibited by high concentrations of amiloride. Alterations in membrane permeability and in ion fluxes and/or perturbation in membrane phospholipids may play a role in this transitional activation of the c-fos gene expression in incubated tissue slices in which the cells are not viable and undergo a necrobiotic process.
D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D002412:Cations / Q000494:pharmacology; D003470:Culture Media; D046508:Culture Techniques; D005786:Gene Expression Regulation; D007545:Isoproterenol / Q000494:pharmacology; D051379:Mice; D010042:Ouabain / Q000494:pharmacology; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D016760:Proto-Oncogene Proteins c-fos; D011519:Proto-Oncogenes; D012333:RNA, Messenger / Q000235:genetics*; D013363:Submandibular Gland / Q000502:physiology*
D002412:Cations; D003470:Culture Media; D011518:Proto-Oncogene Proteins; D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger; D000001:Calcimycin; D010042:Ouabain; D007545:Isoproterenol