Appearance of slow-reacting and complement-requiring neutralizing antibody in cattle infected with Akabane virus.
Slow-reacting complement-requiring neutralizing (NT) antibody was detected in sera from cattle 2 weeks after infection with Akabane virus. Bovine sera obtained 3 or 4 weeks after infection contained slow-reacting noncomplement-requiring NT antibody. The slow-reacting complement-requiring NT antibody was sensitive to 2-mercaptoethanol (2-ME), whereas the slow-reacting noncomplement-requiring NT antibody was resistant to 2-ME. The initial phase may represent the IgM response and the later phase a change to IgG. A NT test was developed in which virus-serum mixtures were incubated at 4 degrees C for 48 h and then with complement at 37 degrees C for 60 min; this gave an improved sensitivity over the previous incubation at 37 degrees C for 60 min.
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis*; D002043:Bunyaviridae / Q000276:immunology*; D002044:Bunyaviridae Infections / Q000276:immunology / Q000662:veterinary*; D002417:Cattle; D003165:Complement System Proteins / Q000276:immunology*; D008623:Mercaptoethanol; D009500:Neutralization Tests; D012840:Simbu virus / Q000276:immunology*
D000914:Antibodies, Viral; D008623:Mercaptoethanol; D003165:Complement System Proteins