title: Human platelet electrorotation change induced by activation: inducer specificity and correlation to serotonin release.
abstract: Electrorotation of single platelets was compared with [14C]serotonin release, aggregation and electron microscopy. Activation of washed and degranulated platelets was induced by thrombin, arachidonic acid, collagen, adrenaline, platelet activation factor (PAF), ADP and A23187. A strong correlation between electrorotation decrease and serotonin release was found. Electrorotation did not correlate with aggregation. It was concluded that an increase of the specific conductivity of the platelet membrane by three orders of magnitude (approx. 1.0.10(-7) S.m-1 to 1.0.10(-4) S.m-1) upon activation was responsible for the observed decrease of anti-field rotation and the shift of the first characteristic frequency towards higher values. Electrorotation allowed for time-dependent measurements of activation. Characteristic activation times in the order of minutes were found. There was the following sequence of activators classified by increasing activation time constants: A23187 was the fastest followed by thrombin, collagen, PAF, arachidonic acid, adrenaline, and ADP.
mesh_terms: D000244:Adenosine Diphosphate / Q000494:pharmacology; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000494:pharmacology; D001792:Blood Platelets / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D000001:Calcimycin / Q000494:pharmacology; D002462:Cell Membrane / Q000502:physiology; D003094:Collagen / Q000494:pharmacology; D003594:Cytoplasmic Granules / Q000502:physiology / Q000648:ultrastructure; D004553:Electric Conductivity; D004594:Electrophysiology; D004837:Epinephrine / Q000494:pharmacology; D006801:Humans; D007700:Kinetics; D008854:Microscopy, Electron; D010972:Platelet Activating Factor / Q000494:pharmacology; D010974:Platelet Aggregation; D012701:Serotonin / Q000097:blood*; D013917:Thrombin / Q000494:pharmacology
chemical_list: D001095:Arachidonic Acids; D010972:Platelet Activating Factor; D016718:Arachidonic Acid; D012701:Serotonin; D000001:Calcimycin; D000244:Adenosine Diphosphate; D003094:Collagen; D013917:Thrombin; D004837:Epinephrine