title: Histological changes and some in vitro biological activities induced by lipopolysaccharide from Bacteroides gingivalis.
abstract: The biological activities of lipopolysaccharide from Bacteroides gingivalis 381 (B-LPS) were examined in vivo and in vitro. Intra-oral mucosal injection of B-LPS induced an acute inflammation at the injection site. Intravenous injection of B-LPS induced necrotic lesions with many thrombi in the liver and lymphocytic reduction in the spleen. By immunohistochemical examination, B-LPS was detected in macrophages in the liver, spleen and lymph nodes. In vitro analysis showed that B-LPS was a potent activator of both neutrophils and macrophages in luminol-dependent response and IL-1 secretion from macrophages and was mitogenic to the spleen cells not only from BALB/c mice but also from LPS-non-responder C3H/HeJ mice. Interferon production from human peripheral mononuclear leucocytes was induced, in vitro, by stimulation with B-LPS but not with the other enterobacterial LPS. These findings clarified the various biological activities of B-LPS affecting various cells and tissues, especially neutrophils, macrophages and lymphocytes. The potent inflammability of B-LPS shown in the present study indicates that it is one of the effective agents to induce periodontitis.
mesh_terms: D000818:Animals; D001439:Bacteroides; D005779:Immunodiffusion; D007150:Immunohistochemistry; D007668:Kidney / Q000187:drug effects; D008070:Lipopolysaccharides / Q000032:analysis / Q000276:immunology / Q000633:toxicity*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008198:Lymph Nodes / Q000187:drug effects; D008264:Macrophages / Q000032:analysis / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D009061:Mouth Mucosa / Q000187:drug effects; D009504:Neutrophils / Q000187:drug effects; D013154:Spleen / Q000187:drug effects
chemical_list: D008070:Lipopolysaccharides