Changes in the lectin-binding pattern of PNA-agglutinin and UEA1 during the DMH-induced carcinogenesis in the normal appearing colonic mucosa of the rat.
The purpose of this study was the investigation of changes in the lectin-binding pattern prior to tumour formation in an experimental model. Female Wistar rats were treated with 1,2-dimethylhydrazine (DMH). After 4, 8, 16, 24, 32 and 40 weeks of treatment the lectin-binding-pattern of the colonic mucosa appearing morphologically normal was examined at the caecum, proximal colon, distal colon and rectum, using FITC-conjugated Peanut-agglutinin (PNA) and Ulex europaeus-agglutinin1 (UEA1). In contradistinction to what has been reported earlier by other authors, PNA did not indicate constant cancer-associated mucin changes. In addition, there was no difference in the UEA1-binding between the control animals and the DMH-treated rats. Thus, in the rat there is no specific PNA- and UEA1-binding pattern during tumour induction in the gut.
D019813:1,2-Dimethylhydrazine; D000818:Animals; D003110:Colonic Neoplasms / Q000139:chemically induced / Q000276:immunology* / Q000378:metabolism; D004127:Dimethylhydrazines; D005260:Female; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D005453:Fluorescence; D007413:Intestinal Mucosa / Q000276:immunology / Q000378:metabolism; D037102:Lectins / Q000378:metabolism*; D009077:Mucins / Q000378:metabolism; D019887:Peanut Agglutinin; D037121:Plant Lectins; D051381:Rats; D011919:Rats, Inbred Strains; D013861:Thiocyanates
D004127:Dimethylhydrazines; D005452:Fluoresceins; D037102:Lectins; D009077:Mucins; D019887:Peanut Agglutinin; D037121:Plant Lectins; D013861:Thiocyanates; C036850:Ulex europaeus lectins; D016650:Fluorescein-5-isothiocyanate; D019813:1,2-Dimethylhydrazine