Culture and characterization of bovine mesenteric lymphatic endothelium.
Lymphatic endothelial cells isolated from bovine mesenteric lymphatic vessels were cultured and characterized. Lymphatic endothelial cells grew as a monolayer displaying an elongated morphology in preconfluent primary cultures. When confluent, the cells exhibited a polygonal morphology to form a "cobblestone" pattern previously described for cultured vascular endothelium. All culture lymphatic endothelial cells expressed Factor VIII-related antigen and bound Ulex europaeus I lectin. Ultrastructurally, cultured lymphatic endothelium was characterized by the presence of Weibel-Palade bodies as well as the usual cytoplasmic organelles.
D000818:Animals; D000941:Antigens / Q000032:analysis; D002417:Cattle; D002478:Cells, Cultured; D004727:Endothelium / Q000166:cytology; D005169:Factor VIII / Q000032:analysis / Q000276:immunology; D037102:Lectins / Q000378:metabolism; D008208:Lymphatic System / Q000166:cytology*; D008643:Mesentery; D009940:Organoids / Q000648:ultrastructure; D037121:Plant Lectins; D011956:Receptors, Cell Surface; D011975:Receptors, Mitogen / Q000032:analysis; D014841:von Willebrand Factor
D000941:Antigens; D037102:Lectins; D037121:Plant Lectins; D011956:Receptors, Cell Surface; D011975:Receptors, Mitogen; C053203:Ulex europaeus lectin receptor; C036850:Ulex europaeus lectins; D014841:von Willebrand Factor; D005169:Factor VIII