title: Follicular fluid glycosaminoglycans inhibit degradation of low-density lipoproteins and progesterone production by porcine granulosa cells.
abstract: Porcine granulosa cells were incubated with commercially available glycosaminoglycans (GAGs) or GAGs purified from porcine follicular fluid to evaluate the effects of GAGs on degradation of low-density lipoproteins (LDL) and progesterone production. Commercially available heparin and chondroitin sulfates (CS) as well as follicular CS and heparan sulfate (HS) inhibited degradation of LDL in dose-dependent manners. Doses of follicular CS and HS required to inhibit 50% of the LDL degradation corresponded to concentrations found in follicular fluid (less than 1 mg/ml). Progesterone production was also inhibited in a dose-dependent fashion by follicular GAGs at concentrations found in follicles. The ability of the follicular GAGs to inhibit degradation of LDL could represent a mechanism by which the utilization of LDL-derived sterol is temporarily restricted following permeabilization of the ovulatory follicle. Follicular GAGs may also modulate utilization of apoprotein E-containing high-density lipoproteins in unruptured follicles.
mesh_terms: D000818:Animals; D001826:Body Fluids / Q000502:physiology; D002478:Cells, Cultured; D002809:Chondroitin Sulfates / Q000502:physiology; D002852:Chromatography, Ion Exchange; D005260:Female; D006025:Glycosaminoglycans / Q000502:physiology*; D006107:Granulosa Cells / Q000378:metabolism*; D006497:Heparan Sulfate / Q000502:physiology; D008077:Lipoproteins, LDL / Q000378:metabolism*; D006080:Ovarian Follicle / Q000502:physiology*; D011374:Progesterone / Q000096:biosynthesis*; D013552:Swine
chemical_list: D002809:Chondroitin Sulfates; D006025:Glycosaminoglycans; D006497:Heparan Sulfate; D008077:Lipoproteins, LDL; D011374:Progesterone