[Radioimmunoassay, in Ascidiella aspersa, of a gonadoliberin (GnRH)- like factor with an apparent molecular weight higher than that of the mammalian decapeptide].
A radiommunoassay (RIA) for mammalian gonadoliberin (mGnRH) showed the presence of a GnRH-like factor in the neural complex of a Protochordate, Ascidiella aspersa (about 0,6 pg eq mGnRH/complex). The slope of the displacement curves was slightly lower than with mGnRH indicating antigene differences. No cross reactive material was found in mantle and siphonal area. The KD on Sephadex G25 was 0.45 versus 0.90 with mGnRH. That suggests that the molecular weight of the Ascidian GnRH-like factor is higher than that of known Vertebrate GnRH's, possibly due to a different processing of the precursor.
D000818:Animals; D008970:Molecular Weight; D009420:Nervous System / Q000032:analysis; D010906:Pituitary Hormone-Releasing Hormones / Q000032:analysis*; D011863:Radioimmunoassay
D010906:Pituitary Hormone-Releasing Hormones
Dosage radioimmunologique, chez Ascidiella aspersa, d'un facteur de type gonadolibérine (GnRH) de poids moléculaire apparent supérieur à celui du décapeptide mammalien.