Direct single-reagent fluorescence polarisation immunoassay for valproic acid in serum.
A fluorescence polarisation immunoassay for quantitating serum concentrations of valproic acid was developed and validated. Its low molecular weight and lack of structural features caused difficulties in producing suitable antibodies. However, success was achieved using 2-propyl-6-aminohexanoic acid to make the fluorescein-labelled drug and two immunogens, the first using glutaraldehyde to link the drug derivative to keyhole limpet haemocyanin, and the second by carbodiimide activation of cellulose hydroxyl groups and coupling them to the drug derivative and killed Mycobacteria. It was found that both immunogens produced a good antibody response in sheep. The antibodies were highly specific and the assay results correlated well with an in-house gas-liquid chromatographic method.
D002849:Chromatography, Gas; D005454:Fluorescence Polarization; D005455:Fluorescent Antibody Technique; D006801:Humans; D014635:Valproic Acid / Q000097:blood*
D014635:Valproic Acid