Establishment of an immature mast cell line from a patient with mast cell leukemia.
A cell line showing many characteristics of immature mast cells has been established from the peripheral blood of a patient with mast cell leukemia. Cultured cells contain low levels of histamine, are stained metachromatically by toluidine blue, and contain chloroacetate esterase, aminocaproate esterase and tryptase activities. The cells lack T and B lymphocyte, as well as myeloid cell markers, and do not possess IgE receptors. Solid tumors of metachromatically positive cells have been successfully induced and serially passed in nude mice using 5-azacytidine transformed cells. This cell line may be useful for future studies of mast cells and their constituents.
D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D002460:Cell Line; D002999:Clone Cells; D006801:Humans; D007946:Leukemia, Mast-Cell / Q000235:genetics / Q000473:pathology*; D008407:Mast Cells; D051379:Mice; D008819:Mice, Nude; D008875:Middle Aged; D009368:Neoplasm Transplantation; D014178:Translocation, Genetic
D000954:Antigens, Surface