Electro-stimulated transformation of E. coli cells pretreated by EDTA solution.
The phenomenon of transformation of E. coli cells under electric treatment has been studied. The cells of strains MH 1, HB 101 and DH 1 after EDTA treatment in an isotonic medium were transformed with DNA pBR322 by applying a single exponential pulse (E = 10 kV/cm, T = 1.5 ms) to the suspension. The maximum transformation efficiency obtained was 4 X 10(6) colonies/micrograms DNA. The maximum transformation frequency was 0.4% at a DNA concentration of 15 micrograms/ml.
D004269:DNA, Bacterial / Q000235:genetics; D004492:Edetic Acid / Q000494:pharmacology*; D004560:Electricity; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics*; D010957:Plasmids; D014169:Transformation, Bacterial / Q000187:drug effects
D004269:DNA, Bacterial; D004492:Edetic Acid