title: Reversible arrest of proliferation of rat 3Y1 fibroblasts in both the G1 and G2 phases by trichostatin A.
abstract: Proliferation of rat 3Y1 cells was found to be specifically blocked by trichostatin A (TSA) at two distinct stages in the cell cycle. The first block occurred in the early G1 phase at least 9 h before the G1/S boundary, whereas the second occurred during the G2 phase. When TSA-arrested cells at the G2 phase were released from the inhibition, the cells with 4C DNA entered a new S phase without passage through the M phase, resulting in the formation of proliferative tetraploid cells. The removal of TSA induced a rapid transient increase in the transcription of c-fos and the cells required 15 h to enter the S phase after release. These results suggest that the cells arrested with TSA are quiescent (G0).
mesh_terms: D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002999:Clone Cells; D004247:DNA / Q000096:biosynthesis; D004622:Embryo, Mammalian; D005347:Fibroblasts / Q000166:cytology*; D005786:Gene Expression Regulation / Q000187:drug effects; D006877:Hydroxamic Acids / Q000494:pharmacology; D007399:Interphase / Q000187:drug effects*; D007700:Kinetics; D008938:Mitosis / Q000187:drug effects; D011123:Polyploidy; D011518:Proto-Oncogene Proteins / Q000235:genetics; D016760:Proto-Oncogene Proteins c-fos; D051381:Rats
chemical_list: D006877:Hydroxamic Acids; D011518:Proto-Oncogene Proteins; D016760:Proto-Oncogene Proteins c-fos; C012589:trichostatin A; D004247:DNA