title: Light microscopy of GTP-binding protein (Go) immunoreactivity within the retina of different vertebrates.
abstract: To examine species differences in the distribution pattern of guanosine triphosphate (GTP)-binding protein (Go) within the vertebrate retina, paraffin-embedded retinae from a number of vertebrate species, including the goldfish, frog, turtle, chicken, monkey, and human, were immunohistochemically stained with affinity-purified antibody against the alpha-subunit of Go. Go-immunoreactive products were found to be located in the neuropil, but not in the cell bodies of neurons, in the retina of all these species. However, some species differences were observed. In the frog, monkey and human, the inner plexiform layer (IPL) was homogeneously stained with this antibody, but in the goldfish, turtle and chicken, the IPL was heterogeneously stained. In the frog, chicken, turtle and human, the outer plexiform layer (OPL) was densely stained with this antibody, but in the goldfish and monkey, the OPL was rather faintly immunoreactive to the antibody. In the goldfish, monkey and human, the outer nuclear layer (ONL) was not immunoreactive to the Go-antibody, whereas in the frog, turtle and chicken, the ONL was immunoreactive to it. The implications of these species differences in Go localization in the vertebrate retina are discussed.
mesh_terms: D000818:Animals; D002645:Chickens; D019204:GTP-Binding Proteins / Q000032:analysis*; D006054:Goldfish; D006801:Humans; D007150:Immunohistochemistry; D008251:Macaca; D012160:Retina / Q000032:analysis* / Q000033:anatomy & histology; D013045:Species Specificity; D014426:Turtles; D014982:Xenopus laevis
chemical_list: D019204:GTP-Binding Proteins