Galactosyltransferase and sialyltransferase are located in different subcellular compartments in HeLa cells.
Galactosyl- and sialyltransferase have been localized by double immunofluorescence labeling in HeLa cells. Galactosyltransferase was found in a compact juxtanuclear structure previously shown to represent the Golgi apparatus (J. Roth and E.G. Berger (1982) J. Cell Biol. 93, 223-9), whereas sialyltransferase was localized to vesicles spread over the whole cytoplasm. These findings indicate different compartments for both transferases and support a model of subcompartmentation of glycosylation steps along the secretory pathway.
D002451:Cell Compartmentation; D005455:Fluorescent Antibody Technique; D005700:Galactosyltransferases / Q000378:metabolism*; D006023:Glycoproteins / Q000096:biosynthesis; D006056:Golgi Apparatus / Q000201:enzymology*; D006367:HeLa Cells / Q000201:enzymology* / Q000648:ultrastructure; D006801:Humans; D011499:Protein Processing, Post-Translational; D012799:Sialyltransferases / Q000378:metabolism*; D000094802:beta-D-Galactoside alpha 2-6-Sialyltransferase
D006023:Glycoproteins; D005700:Galactosyltransferases; D012799:Sialyltransferases; D000094802:beta-D-Galactoside alpha 2-6-Sialyltransferase