title: Mapping and characterization of two mutations to antibiotic supersusceptibility in Pseudomonas aeruginosa.
abstract: Two mutations associated with antibiotic supersusceptibility in Pseudomonas aeruginosa strain Z61 were transferred separately into strain PAO222, using R68.45-mediated conjugation and phage F116L transduction. One mutation (absA) was 40% contransducible with pro-82 at 26 min on the P. aeruginosa chromosome and was associated with increased susceptibility to beta-lactams, gentamicin and hydrophobic agents. Strains carrying the absA mutation also displayed enhanced uptake of a hydrophobic fluorescent probe, 1-N-phenylnaphthylamine, and were found, by SDS-PAGE, to be altered in the pattern of lipopolysaccharide O-antigen distribution. The other mutation (absB), associated with increased susceptibility to beta-lactams and gentamicin but not to hydrophobic agents, was cotransducible with met-28 and proC at 20 min on the chromosome. The absB mutation caused a structurally undefined alteration in the physical interaction of EDTA and gentamicin with the outer membrane.
mesh_terms: D015057:1-Naphthylamine / Q000031:analogs & derivatives / Q000493:pharmacokinetics; D002228:Carbenicillin / Q000494:pharmacology; D002463:Cell Membrane Permeability; D002874:Chromosome Mapping; D004492:Edetic Acid / Q000494:pharmacology; D005839:Gentamicins / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D009154:Mutation; D011112:Polymyxin B / Q000494:pharmacology; D011550:Pseudomonas aeruginosa / Q000187:drug effects*; D014295:Trimethoprim / Q000494:pharmacology
chemical_list: D005839:Gentamicins; C005444:N-phenyl-1-naphthylamine; D015057:1-Naphthylamine; D004492:Edetic Acid; D014295:Trimethoprim; D002228:Carbenicillin; D011112:Polymyxin B