Gonadotropin measurements with fast, non-radioactive methods.
Different analytical methods for the measurement of lutropin and follitropin were compared. Lutropin concentrations of urine samples were measured with the semiquantitative HI Gonavis test (Moshida, Japan), and quantitatively by a modified Ovustick procedure (Monoclonal Antibodies Inc., USA) and by DELFIA (LKB Wallac, Finland). Semiquantitative and quantitative procedures showed conformity in 37 of 40 menstrual cycles when lutropin analyses were performed daily. Values from the analysis of serum samples by dissociation enhanced lanthanide fluoroimmunoassay (DELFIA) and Radioimmunoassay (Serono, FRG) revealed a high degree of correlation (lutropin r = 0.970, follitropin r = 0.994). Immunologic analysis with enzyme- or europium-labelled tracer is a practicable alternative to haemagglutination or radioimmunoassay procedures.
D005260:Female; D005455:Fluorescent Antibody Technique; D005640:Follicle Stimulating Hormone / Q000097:blood; D006062:Gonadotropins / Q000032:analysis*; D006386:Hemagglutination Tests; D006801:Humans; D007124:Immunoenzyme Techniques; D007986:Luteinizing Hormone / Q000097:blood; D010060:Ovulation; D011863:Radioimmunoassay
D006062:Gonadotropins; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone