title: A study on immunodiagnosis of gnathostomiasis by ELISA and double diffusion with special reference to the antigenicity of Gnathostoma doloresi.
abstract: In order to diagnose gnathostomiasis immunologically, Gnathostoma doloresi was evaluated for the antigenicity in comparison with G. hispidum which was recently reported in Japan by using micro-ELISA. The study revealed that G. doloresi can be used as the alternate source of antigen in the test. A significant increase of specific IgG antibodies was seen in 22 (73.3%) out of 30 gnathostomiasis cases. Although double diffusion was slightly less sensitive than ELISA, it was considered more specific than the latter method.
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000032:analysis*; D000947:Antigens, Helminth / Q000276:immunology*; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D005189:False Positive Reactions; D006039:Gnathostoma / Q000276:immunology*; D006801:Humans; D005779:Immunodiffusion; D009349:Nematode Infections / Q000175:diagnosis* / Q000276:immunology; D011237:Predictive Value of Tests; D013802:Thelazioidea / Q000276:immunology*
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth