title: Antigen-specific production of colony-stimulating factors by Listeria monocytogenes-immune, L3T4-positive cells.
abstract: We investigated production of colony-stimulating factors by Listeria monocytogenes-immune spleen cells. Levels of total colony-stimulating factors in supernatants from antigen-stimulated immune cells were increased two- to fourfold over those in supernatants from nonimmune cells. Immune supernatants primarily induced formation of granulocyte colonies, whereas nonimmune supernatants induced formation of macrophage colonies. Immune supernatants had two- to 10-fold higher levels of macrophage colony-stimulating factor, as determined by radioimmunoassay, and higher levels of interleukin-3 and possibly granulocyte-macrophage colony-stimulating factor, as determined by factor-dependent cell line growth, than did nonimmune supernatants. Using enrichment and depletion techniques we showed that L3T4-positive T lymphocytes were responsible for most of the colony-stimulating factor production in the immune reaction.
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology*; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D003115:Colony-Stimulating Factors / Q000096:biosynthesis*; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D006098:Granulocytes; D006133:Growth Substances / Q000096:biosynthesis; D007114:Immunization; D007377:Interleukin-3 / Q000096:biosynthesis; D008089:Listeria monocytogenes / Q000276:immunology; D008088:Listeriosis / Q000276:immunology*; D008264:Macrophages; D051379:Mice; D008810:Mice, Inbred C57BL; D011863:Radioimmunoassay; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism
chemical_list: D000942:Antigens, Bacterial; D003115:Colony-Stimulating Factors; D006133:Growth Substances; D007377:Interleukin-3; D016178:Granulocyte-Macrophage Colony-Stimulating Factor