Resistance of Pseudomonas aeruginosa to beta-lactam antibiotics.
A series of experiments were performed with P. aeruginosa to demonstrate which of the biochemical mechanisms are responsible for the resistance to the beta-lactam antibiotics. The constitutive beta-lactamase was isolated and characterized for the strain used as type OXA whose pI was 7.1, with a molar mass of 49 kg/mol. The strain was also submitted to a series of treatments with Tris and Tris-EDTA to disrupt the outer membrane. The treated cells demonstrated a ten-fold reduction in the MIC with cloxacillin, six-fold with penicillin, and five-fold with oxacillin. At least two different biochemical mechanisms were responsible for the resistance to the beta-lactams studied which could be important in the prevalence of P. aeruginosa in nosocomial infections.
D000900:Anti-Bacterial Agents / Q000378:metabolism / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000032:analysis; D002462:Cell Membrane / Q000378:metabolism; D002463:Cell Membrane Permeability; D004352:Drug Resistance, Microbial; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000378:metabolism; D001618:beta-Lactamases / Q000032:analysis; D047090:beta-Lactams
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D047090:beta-Lactams; D001618:beta-Lactamases