Depletion of mycoplasma from infected cell lines by limiting dilution in 6-methylpurine deoxyriboside.
Cells contaminated with mycoplasma were cloned in the presence of 6-methylpurine deoxyriboside. The results showed that a limiting dilution in the culture medium containing the chemical efficiently depletes mycoplasma providing the cells are sensitive to 0.6-1.2 microM 6-methylpurine.
D000818:Animals; D002460:Cell Line; D003470:Culture Media / Q000494:pharmacology; D004355:Drug Stability; D007941:Leukemia P388 / Q000382:microbiology; D008264:Macrophages / Q000187:drug effects / Q000382:microbiology; D009174:Mycoplasma / Q000187:drug effects* / Q000201:enzymology; D011684:Purine Nucleosides / Q000494:pharmacology*; D011683:Purine-Nucleoside Phosphorylase / Q000378:metabolism; D051381:Rats; D013602:T-Lymphocytes, Cytotoxic / Q000187:drug effects / Q000382:microbiology
D003470:Culture Media; D011684:Purine Nucleosides; C032885:6-methylpurine 2'-deoxyriboside; C019603:adenosine phosphorylase; D011683:Purine-Nucleoside Phosphorylase