Anti-idiotypic antibodies: preparation and identification; application to the detection of antigens of Plasmodium falciparum.
Anti-idiotypic antibodies (anti 94D1Id and anti 94C3Id) were prepared by immunizing rabbits with designated monoclonal antibodies (MoAb) 94D1MoAb (94D1Id) and 94C3MoAb (94C3Id) specific for Plasmodium falciparum. The specificity of both was directed against idiotypes of the relevant MoAb. The interaction between 94D1Id and anti94D1Id or 94C3Id and anti94C3Id was almost completely inhibited when sufficient concentration of P. falciparum antigen was added, suggesting that the idiotypes recognized by anti94D1Id or anti94C3Id were located in the antigen binding site of the MoAbs or the construction of the V region of anti-idiotypic antibodies may be similar to that of P. falciparum antigenic determinants combining with MoAb. Each 4i assay (inhibition of idiotype-anti-idiotype interaction) had a different specificity and sensitivity in detecting P. falciparum asexual antigens. The 94C3Id-anti94C3Id system detected a minimal level of 0.001% parasitaemia and did not cross-react with other species, suggesting that 94C3Id-anti94C3Id system is species specific and may become a valuable tool for immunodiagnosis.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000913:Antibodies, Protozoan / Q000276:immunology*; D000936:Antigen-Antibody Complex / Q000032:analysis; D000953:Antigens, Protozoan / Q000032:analysis*; D005779:Immunodiffusion; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010963:Plasmodium falciparum / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan; D000936:Antigen-Antibody Complex; D000953:Antigens, Protozoan; D007130:Immunoglobulin Idiotypes