[Cloning of the structural gene for diphtheria toxin from Corynebacterium diphtheriae PW8 and expression of its deletion derivative in E. coli].
The coding region of diphtheria toxin gene from Corynebacterium diphtheriae PW8 was cloned in Escherichia coli on pUC19 plasmid. 5' end of the gene was deleted. The deleted gene codes for a toxin like protein truncated for 36 N-terminal aminoacids. The modified gene was fused with 5' terminal part of the lacZ gene on pUC19 plasmid having kept the translational frame intact. The hybrid gene transcription depends on lacP promoter, Mr of the resulting toxoid is 54,000. The toxoid is devoid of ADP-ribosyltransferase activity peculiar for diphtheria toxin and thus is not toxic. The constructed version of the toxin gene can be used for production of diphtheria anatoxin which does not require to be inactivated by formalin.
D002872:Chromosome Deletion; D003001:Cloning, Molecular; D003353:Corynebacterium diphtheriae / Q000235:genetics*; D004167:Diphtheria Toxin / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D005786:Gene Expression Regulation; D005796:Genes; D005798:Genes, Bacterial; D010957:Plasmids
D004167:Diphtheria Toxin
Klonirovanie strukturnoĭ chasti gena difteriĭnogo toksina iz Corynebacterium diphtheriae PW8 i ékspressiia ego deletsionnogo proizvodnogo v E. coli.