title: Antisense ribosomal protein gene expression specifically disrupts oogenesis in Drosophila melanogaster.
abstract: To assess the functional importance of ribosomal protein rpA1 gene expression during development of Drosophila melanogaster, we have transformed into the fly's genome an antisense rpA1 gene driven by a heat shock promoter. Antisense rpA1 expression severely disrupted oogenesis and produced a "small egg" female-sterile phenotype. The severities of these defects were proportional to the level of antisense rpA1 expression. Anti-rpA1 expression did not affect larval or pupal development. Quantitative RNA analysis suggested that high anti-rpA1 expression results in a general decrease of mRNA in the ovary.
mesh_terms: D000818:Animals; D004331:Drosophila melanogaster; D005786:Gene Expression Regulation; D006360:Heat-Shock Proteins / Q000235:genetics; D009866:Oogenesis; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000032:analysis; D012269:Ribosomal Proteins / Q000235:genetics*
chemical_list: D006360:Heat-Shock Proteins; D012333:RNA, Messenger; D012269:Ribosomal Proteins