Molecular mechanism for the formation of the high-affinity complex of interleukin 2 and its receptor.
Interleukin 2 (IL-2) binds to its receptors with three distinct affinities, with Kd values of 10(-11) M (high), 10(-9) M (intermediate) and 10(-8) M (low). IL-2 responding cells express two proteins that bind IL-2, i.e. a 55 x 10(3) Mr protein (p55 or L chain), which has classically been known as the IL-2 receptor and a second 75 x 10(3) Mr chain (p75 or H chain) with intermediate affinity. Experiments were performed to clarify the mechanism of the high-affinity site formation. Crosslinking of human IL-2 with the high-affinity sites of human T lymphocytes yielded a 150 x 10(3) Mr ternary complex consisting of IL-2, L and H chains. The ternary complex with human IL-2 was formed on EL/Tac 3 cells expressing human L and murine H chains, although human IL-2 was unable to bind to the parental EL-4 cell, which does not express human L chain. The high-affinity ternary complex was stable during solubilization and fractionated by gel-filtration chromatography, and the numbers of these complexes were quantified by this method. The number of high-affinity sites on the CT/hR-1 cells, which express the human L, murine L and murine H chains, was almost constant even when either the human or murine L chain was blocked by specific antibodies in agreement with a previous observation. These results indicate that the L and H chains do not form a stable binary complex by themselves and that IL-2 binding induces the formation of the stable high-affinity ternary complex.
D000818:Animals; D002460:Cell Line; D006801:Humans; D007376:Interleukin-2 / Q000378:metabolism*; D051379:Mice; D008954:Models, Biological; D011485:Protein Binding; D011971:Receptors, Immunologic / Q000378:metabolism*; D015375:Receptors, Interleukin-2; D013601:T-Lymphocytes / Q000378:metabolism
D007376:Interleukin-2; D011971:Receptors, Immunologic; D015375:Receptors, Interleukin-2