A new approach to studies of cell-antibody interactions using fluorescent lipid probes.
The interaction of poly- and monoclonal antibodies against the L-chain of human Ig with Burkitt lymphoma EB-3 cells was studied using a fluorescent lipid probe, anthrylvinyl-labelled sphingomyelin, incorporated into the cell plasma membrane. Binding of the antibodies to Ig receptors on the surface was shown to induce changes in the fluorescence polarization of the probe. The high sensitivity of the method allows one to detect less than 100 antibody molecules per cell. The possibility of using cells or liposomes carrying antigens and fluorescent lipids for the determination of antibodies in solution is discussed.
D000888:Antibodies, Anti-Idiotypic; D000937:Antigen-Antibody Reactions; D002051:Burkitt Lymphoma; D002462:Cell Membrane / Q000276:immunology*; D005454:Fluorescence Polarization / Q000379:methods*; D005456:Fluorescent Dyes; D006801:Humans; D007147:Immunoglobulin Light Chains; D008563:Membrane Lipids; D011947:Receptors, Antigen, B-Cell; D013109:Sphingomyelins; D014407:Tumor Cells, Cultured
D000888:Antibodies, Anti-Idiotypic; D005456:Fluorescent Dyes; D007147:Immunoglobulin Light Chains; D008563:Membrane Lipids; D011947:Receptors, Antigen, B-Cell; D013109:Sphingomyelins