Nerve growth factor, a differentiating agent, and epidermal growth factor, a mitogen, increase the activities of different S6 kinases in PC12 cells.
Treatment of PC12 cells with either nerve growth factor, a differentiating agent, or epidermal growth factor, a mitogen, leads to an increase in the phosphorylation of the ribosomal protein S6. The soluble fraction of PC12 cells contains two S6 kinases, separable on heparin-Sepharose. Treatment of the cells with nerve growth factor results in an increase in the activity of one of these kinases; treatment of the cells with epidermal growth factor results in an increase in the activity of the other one. The data suggest that the patterns of phosphorylation and, in turn, the functional properties of S6 are different in cells instructed to differentiate from those in cells instructed to divide.
D000310:Adrenal Gland Neoplasms; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D002846:Chromatography, Affinity; D004533:Egtazic Acid / Q000494:pharmacology; D004789:Enzyme Activation; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D005994:Glycerophosphates / Q000494:pharmacology; D007700:Kinetics; D008934:Mitogens; D009414:Nerve Growth Factors / Q000494:pharmacology*; D010673:Pheochromocytoma; D010766:Phosphorylation; D011494:Protein Kinases / Q000302:isolation & purification / Q000378:metabolism*; D019893:Ribosomal Protein S6 Kinases
D005994:Glycerophosphates; D008934:Mitogens; D009414:Nerve Growth Factors; D004533:Egtazic Acid; D004815:Epidermal Growth Factor; D011494:Protein Kinases; D019893:Ribosomal Protein S6 Kinases; C031463:beta-glycerophosphoric acid