[Effect of RO 15-1788 and RO 5-4684 on the evoked activity of neurons in hippocampal sections].
The action of specific benzodiazepine (BD) antagonist R015-1788 and peripheral benzodiazepine receptor (BDR) ligand R05-4864 on the evoked activity of hippocampal neurons was studied using brain slice method. The extracellular activity was registered in CAI area upon single and paired pulse stimulation of Schaffer collaterals. R015-1788 application (5 microM, for 3-6 min) reduced paired pulse inhibition (PPI). More prolonged application produced a depression of the population spike (PS). R015-1788 (5 microM) blocked diazepam (2 microM), hexobarbital (10 microM) and GABA (40 microM) potentiation of PPI. Interaction of R015-1788 with endogenous BD-like ligand as a possible explanation for the effects under study is discussed. R05-4864 (10 microM) reduced PPI and decreased PS evoked by single pulse stimulation. Frequency stimulation revealed the generation of additional PS after drug application. The data presented suggest that suppression of hippocampal inhibitory circuits may be a general feature of anxiogenic BDR ligands.
D000818:Animals; D001570:Benzodiazepinones / Q000494:pharmacology*; D005071:Evoked Potentials / Q000187:drug effects; D005442:Flumazenil / Q000494:pharmacology*; D006624:Hippocampus / Q000187:drug effects* / Q000502:physiology; D066298:In Vitro Techniques; D051381:Rats
D001570:Benzodiazepinones; C028513:4'-chlorodiazepam; D005442:Flumazenil
Deĭstvie RO15-1788 i RO5-4684 na vyzvannuiu aktivnost' neĭronov srezov gippokampa.