The action of carbaryl and its metabolite alpha-naphthol on mitosis in V79 Chinese hamster fibroblasts. Indications of the involvement of some cholinester in cell division.
Carbaryl (1-naphthyl-N-methylcarbamate) is a spindle-disturbing agent. A number of effects probably contribute to this activity. Carbaryl efficiently lowers the intracellular level of glutathione, thus endangering the integrity of the spindle. Carbaryl also causes lipid peroxidation. With anti-oxidant pretreatment, the frequency of c-mitotic cells is lowered which indicates that lipid peroxidation is a partial cause of the spindle-disturbing activity. At high concentrations of carbaryl, monopolar configurations in combination with cleavage are frequent and when tested, alpha-naphthol, which is thought to be formed from carbaryl in significant amounts at these concentrations, also gave monopolar configurations but with significantly lower frequencies of concomitant cleavage. Carbaryl inhibits cholinesterases and when tested, another cholinesterase inhibitor, diisopropylfluorophosphate, in combination with alpha-naphthol also increased the frequency of monopolar configuration in combination with cleavage. We therefore propose the involvement of some cholinester in the process of cell division.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D012721:Carbaryl / Q000494:pharmacology*; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D005947:Glucose / Q000494:pharmacology; D007531:Isoflurophate / Q000494:pharmacology; D015227:Lipid Peroxidation; D008938:Mitosis / Q000187:drug effects*; D009284:Naphthols / Q000494:pharmacology*; D020887:Selenious Acid; D012643:Selenium / Q000494:pharmacology; D013438:Sulfhydryl Compounds / Q000378:metabolism; D014810:Vitamin E / Q000494:pharmacology
D009284:Naphthols; D013438:Sulfhydryl Compounds; D007531:Isoflurophate; D014810:Vitamin E; D000255:Adenosine Triphosphate; D020887:Selenious Acid; D012643:Selenium; D005947:Glucose; D012721:Carbaryl