Composition of alginate synthesized during the growth cycle of Pseudomonas aeruginosa.
A mucoid P. aeruginosa isolated from the sputum of a cystic fibrosis patient was grown in batch culture on a complex medium. During the growth cycle the amount of alginate produced was estimated and its composition was determined by proton magnetic resonance (1H-n.m.r) spectroscopy. Exopolysaccharide production occurred mainly during the exponential phase of growth. The alginate samples isolated varied little in composition and were characterized by being highly acetylated, high mannuronate (0.83-0.93 mole fraction) polymers. Guluronate was present only within heteropolymeric regions of the polysaccharides which all displayed a complete absence of polyguluronate. Ca2+ ion supplementation of the medium was not observed to increase the levels of guluronate in the alginates produced.
D000464:Alginates / Q000302:isolation & purification / Q000378:metabolism*; D002118:Calcium / Q000494:pharmacology; D003550:Cystic Fibrosis / Q000382:microbiology; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D011550:Pseudomonas aeruginosa / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D013183:Sputum / Q000382:microbiology
D000464:Alginates; D006603:Hexuronic Acids; D020723:Glucuronic Acid; D002118:Calcium