Melatonin biosynthesis in Drosophila: its nature and its effects.
Drosophila melanogaster homogenates incubated with tritiated 5-hydroxytryptophan, 5-hydroxytryptamine, or N-acetylserotonin have the ability of converting them into labelled indolamines, including melatonin. All these compounds were characterized by two-dimensional thin-layer chromatography by comparison with nonradiolabelled standards, and melatonin by mass spectrometry and radioimmunoassay. On the other hand, the injection of pharmacological doses of melatonin into 2-day-old female flies diminishes the mating speed and the oviposition rate.
D006916:5-Hydroxytryptophan / Q000378:metabolism; D000818:Animals; D002855:Chromatography, Thin Layer; D004330:Drosophila / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000378:metabolism; D005260:Female; D007211:Indoles / Q000378:metabolism; D008297:Male; D013058:Mass Spectrometry; D008550:Melatonin / Q000096:biosynthesis* / Q000494:pharmacology; D010058:Oviposition / Q000187:drug effects; D011863:Radioimmunoassay; D012701:Serotonin / Q000031:analogs & derivatives / Q000378:metabolism; D012726:Sexual Behavior, Animal / Q000187:drug effects
D007211:Indoles; D012701:Serotonin; D006916:5-Hydroxytryptophan; D008550:Melatonin; C006389:N-acetylserotonin