Sites of termination of in vitro DNA synthesis on cis-diamminedichloroplatinum(II) treated single-stranded DNA: a comparison between E. coli DNA polymerase I and eucaryotic DNA polymerases alpha.
We have compared the capacity of the large fragment of E. coli DNA polymerase I and highly purified DNA polymerases alpha from either Drosophila melanogaster embryos or calf thymus to replicate single-stranded M13 mp10 DNA treated with the antitumoral drug cis-diamminedichloroplatinum(II) (cis-DDP). We report that: a) although prokaryotic and eukaryotic enzymes have different structural complexity and dissimilar in vivo functions, their synthesis was blocked in vitro at similar sites on cis-DDP treated DNA; b) this inhibition occurred not only at d(G)n sequences, as previously reported for E. coli DNA polymerase I, (Pinto & Lippard (1985) Proc. Natl. Acad. Sci. USA, 82, 4616-4619) but also at other sequences which may represent putative cis-DDP-DNA adducts.
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002945:Cisplatin / Q000494:pharmacology*; D004249:DNA Damage; D004256:DNA Polymerase I / Q000378:metabolism*; D004257:DNA Polymerase II / Q000378:metabolism*; D004261:DNA Replication / Q000187:drug effects*; D004277:DNA, Single-Stranded / Q000187:drug effects / Q000235:genetics*; D004331:Drosophila melanogaster / Q000196:embryology / Q000201:enzymology; D004625:Embryo, Nonmammalian / Q000201:enzymology; D004926:Escherichia coli / Q000201:enzymology*; D008969:Molecular Sequence Data; D013698:Templates, Genetic; D013950:Thymus Gland / Q000201:enzymology
D004277:DNA, Single-Stranded; D004256:DNA Polymerase I; D004257:DNA Polymerase II; D002945:Cisplatin