[Suitability of thermal selection for the primary culture of Pseudomonas aeruginosa].
It could be shown by quantitative methods that growing P. aeruginosa suspensions at 42 degrees C without any loss is possible only in a medium without any inhibitory additives--when suspensions of unimpaired organisms are used. The addition of inhibitors e.g. malachite green without influence to the growth rate of P. aeruginosa at 37 degrees C leads to a marked reduction of growth at temperatures only slightly higher e.g. 38 degrees C. This can also be observed when poor media like Drake's medium 10 are used. The method of thermic selection was the most inappropriate for the culture of P. aeruginosa organisms impaired by chlorine. Germs which showed good growth at 37 degrees C could be grown only at a very reduced rate at 42 degrees C even though a rich medium free of inhibitory additives was used. Thermic selection is not suited to the cultivation of P. aeruginosa from samples of water which as a rule is chlorinated e.g. swimming pool water.
D001431:Bacteriological Techniques; D003470:Culture Media; D006801:Humans; D011550:Pseudomonas aeruginosa / Q000254:growth & development*; D013551:Swimming Pools; D013696:Temperature; D014871:Water Microbiology
D003470:Culture Media
Zur Eignung der thermischen Selektion für die Primärkultur von Pseudomonas aeruginosa.