Inhalation of methyl bromide gas induces mitotic recombination in somatic cells of Drosophila melanogaster.
The fumigant methyl bromide was evaluated for genotoxicity in the somatic wing-spot assay of Drosophila melanogaster. Third instar larvae trans-dihybrid for mwh and flr3 were exposed to varying concentrations (0-16 mg/l) of the gas for 1 h. Following this exposure via inhalation, the larvae were placed into vials containing Instant Medium. 7 days after the exposure, the adult flies in the vials were collected, and their wings were scored under 400X magnification for the presence of clones of cells possessing malformed wing-hairs. Such clones appeared as mwh-flr3 twin spots and single spots of either mwh or flr3 phenotype. Exposure to methyl bromide was found to result in the positive induction of both twin spots and large (greater than 2 cells) single spots. For each endpoint, a significant exponential association was obtained between concentration and frequency of spots per wing. Methyl bromide was found to be a negative inducer of small (1-2 cells) single spots at all concentrations except 16 mg/l where a positive effect was observed. Because twin spots arise exclusively from mitotic recombination, methyl bromide was identified as having recombinogenic activity in the somatic tissue of Drosophila larvae.
D000818:Animals; D004331:Drosophila melanogaster / Q000187:drug effects / Q000235:genetics*; D006842:Hydrocarbons, Brominated / Q000494:pharmacology*; D007306:Insecticides / Q000494:pharmacology; D007814:Larva; D009152:Mutagenicity Tests; D009154:Mutation; D011995:Recombination, Genetic / Q000187:drug effects
D006842:Hydrocarbons, Brominated; D007306:Insecticides; C005218:methyl bromide