In vitro and in vivo behaviour of rat islets of Langerhans treated with MHC antisera and complement.
The in vitro and in vivo behaviour of rat islets of Langerhans pretreated with polyclonal MHC antisera and complement was investigated. Complement-dependent cytotoxicity against rat islets was mediated by the antisera which were either directed against all MHC products ("a anti-u") or against products of the B-region of rat MHC ("Ba anti-Bu"). There were only minor alterations in glucose-stimulated insulin secretion and in 3H-leucine incorporation into islet proteins and into (pro)insulin in the pretreated islets. The syngeneic transplantation of "Ba anti-Bu"-treated islets led to a permanent graft survival in all streptozotocin-diabetic rats but after "a anti-u"-pretreatment permanent graft survival was only achieved in 33% of the syngeneic recipients. It is concluded, that in the latter case a complement-dependent cytotoxicity is exerted against the islets which may not allow sufficient in vivo survival. Now it appears necessary to look for effects of pretreatment prior to an allogeneic transplantation.
D000818:Animals; D003165:Complement System Proteins / Q000276:immunology*; D003602:Cytotoxicity, Immunologic; D007106:Immune Sera / Q000276:immunology*; D007328:Insulin / Q000096:biosynthesis; D007515:Islets of Langerhans / Q000276:immunology*; D016381:Islets of Langerhans Transplantation; D008285:Major Histocompatibility Complex; D008297:Male; D051381:Rats; D011917:Rats, Inbred Lew
D007106:Immune Sera; D007328:Insulin; D003165:Complement System Proteins