Stimulation of murine cultured mast cells under anaerobic conditions: inhibition of arachidonic acid release.
The exocytosis of beta-hexosaminidase from either IgE-antigen- or calcium ionophore A23187-stimulated murine bone-marrow-derived mast cells was not affected by oxygen-depleted conditions regardless of the absence of glucose from the medium. No detectable changes in the content of ATP were observed when the cells were triggered immunologically under anaerobic conditions in the absence of glucose in the medium. Depletion of oxygen from mast cells activated by both stimuli almost completely inhibited the specific release of arachidonic acid, which indicates that arachidonate does not play a significant role in the secretion of preformed mediators.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000332:Aerobiosis; D000693:Anaerobiosis; D000818:Animals; D000941:Antigens; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism*; D000001:Calcimycin / Q000494:pharmacology; D002478:Cells, Cultured; D007073:Immunoglobulin E / Q000276:immunology; D008407:Mast Cells / Q000187:drug effects / Q000276:immunology / Q000502:physiology*; D051379:Mice
D000941:Antigens; D001095:Arachidonic Acids; D016718:Arachidonic Acid; D007073:Immunoglobulin E; D000001:Calcimycin; D000255:Adenosine Triphosphate