Localization of nRNP antigen in mammalian cells stained with peroxidase-labelled IgG fraction of anti-nRNP antibody obtained from a patient with mixed connective tissue disease (MCTD).
The peroxidase-labelled IgG fraction of anti-nRNP antibody obtained from a MCTD patient stained the nuclei of almost any kind of cells of man and rat. In addition, the cytoplasma of anterior horn cells of the rat spinal cord was exclusively reacted with this antibody. Both cytoplasmic and nuclear staining of the anterior horn cells were reduced after treatment of tissue sections with RNase.
D000818:Animals; D000870:Anterior Horn Cells / Q000032:analysis; D000974:Antibodies, Antinuclear; D000941:Antigens / Q000032:analysis*; D006801:Humans; D005779:Immunodiffusion; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G; D008947:Mixed Connective Tissue Disease / Q000276:immunology*; D051381:Rats; D011919:Rats, Inbred Strains; D012261:Ribonucleoproteins / Q000032:analysis*
D000974:Antibodies, Antinuclear; D000941:Antigens; D007074:Immunoglobulin G; D012261:Ribonucleoproteins