Identification of the main gestagen metabolite in marmoset (Callithrix jacchus) urine by NMR and MS spectroscopy.
Hydroxypregnanolone is the most abundant progesterone metabolite in the urine of marmoset monkeys (Callithrix jacchus). The substance is excreted as conjugate. The concentration of this steroid may be monitored by high performance, thin layer chromatography and postchromatographic derivatization. Hydroxypregnanolone was purified and subsequently identified by NMR spectroscopy and gas chromatography/mass spectroscopy. The exact chemical structure is 5 alpha-pregnane-3 alpha, 7 alpha-diol-20-one.
D000818:Animals; D002144:Callithrix / Q000652:urine*; D002143:Callitrichinae / Q000652:urine*; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D005260:Female; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D011247:Pregnancy; D011278:Pregnanes / Q000652:urine*; D011280:Pregnanolone / Q000031:analogs & derivatives / Q000032:analysis / Q000302:isolation & purification / Q000652:urine*
D011278:Pregnanes; C013051:17-hydroxypregnanolone; D011280:Pregnanolone