Preparation of monoclonal antibodies to Theileria sergenti and their reactivity to antigens in experimentally infected cattle.
Two distinct monoclonal antibodies (3-H and 11-D) were produced against Theileria sergenti. These two new products, together with monoclonal antibody 1-G obtained in a previous study, were used to detect the parasites in experimentally infected cattle. During the first period of dexamethasone treatment, which was carried out to increase parasitemia in the infected cattle, the number of erythrocytes detected by 3-H, 11-D and 1-D increased in two experimentally infected calves. During the second period of dexamethasone treatment, the number of infected erythrocytes detected by 3-H and 11-D were similarly increased, but the number of infected erythrocytes detected by 1-G did not increase and infected erythrocytes in one calf were not detected by 1-G.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000953:Antigens, Protozoan / Q000276:immunology; D016782:Apicomplexa / Q000276:immunology*; D002417:Cattle; D004912:Erythrocytes / Q000469:parasitology; D007124:Immunoenzyme Techniques; D013801:Theileriasis / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan