Assay of lipid hydroperoxides by activation of cyclooxygenase activity.
The bis-dioxygenation of arachidonate to form the hydroperoxide, prostaglandin G2, is catalyzed by the cyclooxygenase activity of prostaglandin H synthase. This activity is stimulated by hydroperoxide, and it can be used to assay picomole amounts of hydroperoxide.
D000818:Animals; D004789:Enzyme Activation; D006801:Humans; D008054:Lipid Peroxides / Q000032:analysis*; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism*
D008054:Lipid Peroxides; D011451:Prostaglandin-Endoperoxide Synthases