title: Effects of ultrasound on the catalytic activity of matrix-bound glucoamylase.
abstract: The effect of ultrasonic waves on the activity of glucoamylase bound to a porous polystyrene matrix is investigated in this Paper. The immobilized enzyme was sonated in a flow cuvette at frequencies between 1 and 11 MHz and sound intensities up to 5 kW m-2. The effect was measured as a function of the type and concentration of the substrate, carrier particle size, flow rate of the substrate solution and ultrasonic frequency. The activity increase is discussed in terms of a possible ultrasonic mechanism.
mesh_terms: D002384:Catalysis; D004800:Enzymes, Immobilized / Q000378:metabolism*; D005087:Glucan 1,4-alpha-Glucosidase / Q000378:metabolism*; D005959:Glucosidases / Q000378:metabolism*; D006358:Hot Temperature; D007700:Kinetics; D008320:Maltose / Q000378:metabolism; D010316:Particle Size; D011137:Polystyrenes; D013213:Starch / Q000378:metabolism; D014465:Ultrasonics
chemical_list: D004800:Enzymes, Immobilized; D011137:Polystyrenes; D008320:Maltose; D013213:Starch; D005959:Glucosidases; D005087:Glucan 1,4-alpha-Glucosidase