title: The cellular interactions of laminin fragments. Cell adhesion correlates with two fragment-specific high affinity binding sites.
abstract: The molecular interactions of laminin with several tumor cell lines and skin fibroblasts were investigated by radioligand binding studies and cell attachment assays using laminin, the laminin-nidogen complex, and laminin fragments as substrates and also domain-specific antibodies as inhibitors of cell attachment. The majority of cells showed a dual binding pattern for fragments 1 and 8 which originate from short-arm or long-arm structures of laminin, respectively. Both of these fragments in solution bind to suspended cells with high affinity (KD = 1-10 nM), with the receptor numbers for each fragment depending on the cell type. Competition studies and independent variation of receptor numbers demonstrated that the cell-binding structures on each fragment are different, implicating the existence of two distinct cellular receptors for laminin. The ability of these fragments to act as substrates for cell adhesion correlated with the presence of high affinity binding sites on the cells. However, only antibodies to fragment 8 were able to block cell adhesion to laminin, despite the presence of binding sites for fragment 1. A few cells had very low numbers of high affinity receptors for either fragment 1 or 8. The latter cell type was used to demonstrate that complex formation between laminin and nidogen, which binds to fragment 1 structures, reduces the potential of laminin for cell binding.
mesh_terms: D000818:Animals; D001665:Binding Sites; D002448:Cell Adhesion; D002460:Cell Line; D006224:Cricetinae; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000378:metabolism; D005354:Fibrosarcoma / Q000378:metabolism; D007700:Kinetics; D007797:Laminin / Q000378:metabolism*; D008562:Membrane Glycoproteins; D008565:Membrane Proteins / Q000378:metabolism; D010446:Peptide Fragments / Q000378:metabolism*; D011487:Protein Conformation; D012867:Skin / Q000166:cytology
chemical_list: D007797:Laminin; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D010446:Peptide Fragments; C029967:nidogen