title: Expression of histocompatibility antigens, transferrin receptors, intermediate filaments, and alkaline phosphatase by in vitro cultured rat placental cells and rat placental cells in situ.
abstract: Rat placental cells (RPCs) derived from the chorioallantoic placenta of day-12 Holtzman rats were tested for the expression of class I and class II RT I histocompatibility antigens, transferrin receptors, intermediate filaments, and alkaline phosphatase. The binding of mouse monoclonal antibodies to those antigens by RPCs was compared with the binding of the same reagents to rat placental cells in situ. RPCs expressed low levels of class I antigens and failed to express detectable levels of class II antigens. RPCs resisted up-regulation of expression of class I antigens by interferon-gamma, and did not express class II antigens following exposure to medium containing interferon. Transferrin receptors; cytokeratin intermediate filaments, and alkaline phosphatase were universally expressed by RPCs. Taken together with the patterns of expression of the same antigens by rat placental cells in situ, the results suggest that RPCs comprise labyrinthine trophoblast cells. Those cells may provide a valuable new approach for studying the structures and functions of trophoblast cells in vitro.
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000378:metabolism*; D005260:Female; D005434:Flow Cytometry; D006649:Histocompatibility Antigens / Q000276:immunology*; D007124:Immunoenzyme Techniques; D007371:Interferon-gamma / Q000494:pharmacology; D007382:Intermediate Filaments / Q000378:metabolism*; D010920:Placenta / Q000166:cytology / Q000378:metabolism*; D011247:Pregnancy; D051381:Rats; D011990:Receptors, Transferrin / Q000378:metabolism*
chemical_list: D006649:Histocompatibility Antigens; D011990:Receptors, Transferrin; D007371:Interferon-gamma; D000469:Alkaline Phosphatase