title: An immunocytochemical study of the germinal layer vasculature in the developing fetal brain using Ulex europaeus 1 lectin.
abstract: The characteristics of the germinal matrix vasculature were studied in the developing fetal brain using immunocytochemical methods. A preliminary comparative immunocytochemical study was made on six fetal brains to compare endothelial staining by Ulex europaeus I lectin with that of antibody to Factor VIII related antigen. Ulex was found to stain germinal layer vessels better than Factor VIII related antigen. Subsequently, the germinal layers of a further 15 fetal and preterm infant brains ranging from 13 to 35 weeks' gestation were stained with Ulex europaeus I to demonstrate the vasculature. With increasing gestation, there was a gradual increase in vessel density, particularly of capillaries. This was not a uniform process. A plexus of capillaries was prominent immediately beneath the ependyma while the more central parts of the germinal matrix contained fewer, but often larger diameter, vessels. The variation in vessel density which was a feature of the later gestation brains may have implications for local blood flow and may be a factor in haemorrhage at this site.
mesh_terms: D000941:Antigens / Q000276:immunology; D001921:Brain / Q000098:blood supply / Q000196:embryology*; D002196:Capillaries / Q000196:embryology; D004727:Endothelium; D005169:Factor VIII / Q000276:immunology; D005855:Germ Layers / Q000098:blood supply*; D005865:Gestational Age; D006801:Humans; D007150:Immunohistochemistry; D037102:Lectins; D037121:Plant Lectins; D014841:von Willebrand Factor
chemical_list: D000941:Antigens; D037102:Lectins; D037121:Plant Lectins; C036850:Ulex europaeus lectins; D014841:von Willebrand Factor; D005169:Factor VIII