[Change in immunological and biochemical parameters in germ-free animals in response to T-activin].
The influence of immunoregulating humoral thymus factor T-activin (fraction AFT-6) on the activity of xenobiotic metabolism enzymes (EMX) and cellular-dependent cytotoxicity was investigated in germ-free guinea-pigs. Germ-free and conventional animals were injected 5 micrograms of T-activin intraperitoneally, on 3 successive days. The control animals were injected a physiologic saline. A day after the last injection the animals were killed, and EMX and K cell activity was measured. It was found that EMX activity in germ-free animals was decreased. T-activin stimulated cytochrome P-450, NADP-cytochrome-C-reductase, glutathione-S-transferase, benzopyrene hydroxylase and epoxyhydrase activity. In conventional animals the activity of these enzymes remained unchanged under the influence of T-activin. K-cell activity in germ-free animals was decreased, as compared to conventional guinea-pigs. Under the influence of T-activin the parameter increased and stood at about 70% of normal values.
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D000920:Antibody-Dependent Cell Cytotoxicity; D001579:Benzopyrene Hydroxylase / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D005856:Germ-Free Life / Q000187:drug effects*; D005982:Glutathione Transferase / Q000378:metabolism; D006168:Guinea Pigs; D009251:NADPH-Ferrihemoprotein Reductase / Q000378:metabolism; D010088:Oxidoreductases / Q000378:metabolism; D010455:Peptides / Q000494:pharmacology*; D013949:Thymus Extracts / Q000494:pharmacology*
D000276:Adjuvants, Immunologic; D010455:Peptides; D013949:Thymus Extracts; C036191:T-activin; D003577:Cytochrome P-450 Enzyme System; D010088:Oxidoreductases; C023111:epoxidase; D001579:Benzopyrene Hydroxylase; D009251:NADPH-Ferrihemoprotein Reductase; D005982:Glutathione Transferase
Izmenenie nekotorykh immunologicheskikh i biokhimicheskikh parametrov pod deĭstviem T-aktivina u bezmikrobnykh zhivotnykh.