Changes in MAO activities in several organs of rats after administration of l-thyroxine.
Male rats were given daily injections of 200 micrograms/kg l-thyroxine, s.c., for a period of 10 days. Monoamine oxidase (MAO) activities in the heart, lung and liver mitochondria decreased rapidly to about 50% those of the control rats with 5-HT and beta-phenylethylamine (beta-PEA) as substrates on the first day. After that, heart MAO activity increased gradually and exceeded the control value after 10 days with 5-HT as the substrate. The level of liver MAO activity was maintained at about 50-70% during the same period of administration with 5-HT as the substrate. The thyroxine treated rats showed no marked change in brain MAO activity. In vitro, l-thyroxine and its metabolites had no discriminative actions on MAO activities in these organs of rats. The heart, lung and liver MAO have unaltered Km values for 5-HT and beta-PEA, but decreases in the Vmax for both substrates were observed between the control and l-thyroxine-treated rats. Addition of the brain, heart and liver cytosol fractions from l-thyroxine treated rats caused MAO activities of heart mitochondria to decrease with 5-HT as a substrate and caused them to increase with beta-PEA as a substrate. MAO activities in liver also were inhibited by adding all the cytosols when beta-PEA was the substrate, but on the contrary, lung MAO activities were increased when 5-HT was the substrate. These results indicate the possible presence of multiple modulators of MAO in the cytosol fractions of l-thyroxine treated rats.
D000818:Animals; D001921:Brain / Q000378:metabolism; D003010:Clorgyline / Q000494:pharmacology; D003600:Cytosol / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D008297:Male; D008928:Mitochondria / Q000201:enzymology; D008929:Mitochondria, Heart / Q000201:enzymology; D008930:Mitochondria, Liver / Q000201:enzymology; D008995:Monoamine Oxidase / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012642:Selegiline / Q000494:pharmacology; D013974:Thyroxine / Q000494:pharmacology*
D012642:Selegiline; D008995:Monoamine Oxidase; D003010:Clorgyline; D013974:Thyroxine