Ontogeny of 'macrophage' function. VI. Down-regulation for Ia-expression of newborn mouse macrophages by endogenous beta-interferon.
Peritoneal exudate macrophages (M phi) of newborn mice (NB-M phi) were apparently almost incapable of expressing Ia antigen even if stimulated by IFN-gamma. No significant difference was observed in the number and the affinity of receptors for IFN-gamma between NB-M phi and M phi of adult mice (Ad-M phi). Addition of indomethacin, a prostaglandin synthesis inhibitor, was ineffective in enhancing the Ia-expression of NB-M phi. Responsiveness of NB-M phi to IFN-gamma, however, was disclosed by the addition to the culture of anti-IFN-beta or anti-IFN-alpha/beta, but not anti-IFN-alpha antibody. Responsiveness of NB-M phi to IFN-gamma was not improved by the depletion of fibroblasts from NB-M phi populations. These results strongly argue that Ia-expression of NB-M phi, which is otherwise to be induced by IFN-gamma, is suppressed by IFN-beta derived from NB-M phi themselves.
D000818:Animals; D000831:Animals, Newborn; D000906:Antibodies / Q000008:administration & dosage; D001665:Binding Sites; D005347:Fibroblasts / Q000276:immunology; D000949:Histocompatibility Antigens Class II; D007121:Immunocompetence; D066298:In Vitro Techniques; D007370:Interferon Type I / Q000037:antagonists & inhibitors / Q000276:immunology*; D007371:Interferon-gamma / Q000494:pharmacology; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D008809:Mice, Inbred C3H; D011453:Prostaglandins / Q000276:immunology
D000906:Antibodies; D000949:Histocompatibility Antigens Class II; D007370:Interferon Type I; D011453:Prostaglandins; D007371:Interferon-gamma