Guanine nucleotides inhibit poly-L-arginine-induced membrane damage in polymorphonuclear leukocytes.
Poly-L-arginine induces a strong release of the cytoplasmic enzyme lactate dehydrogenase from rabbit polymorphonuclear leukocytes, indicating that plasma membrane damage occurs. GTP gamma S, a stable guanine nucleotide, completely inhibits poly-L-arginine-induced LDH release whereas pretreatment of the cells with pertussis toxin gives a moderate inhibition. The results suggest that poly-L-arginine-induced plasma membrane damage is mediated by guanine nucleotide binding structures.
D000818:Animals; D002118:Calcium / Q000378:metabolism; D002462:Cell Membrane / Q000187:drug effects* / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000502:physiology; D006150:Guanine Nucleotides / Q000494:pharmacology*; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D006160:Guanosine Triphosphate / Q000031:analogs & derivatives / Q000494:pharmacology; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D009504:Neutrophils / Q000187:drug effects / Q000648:ultrastructure*; D010455:Peptides / Q000494:pharmacology*; D037342:Pertussis Toxin; D011817:Rabbits; D013873:Thionucleotides / Q000494:pharmacology; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
D006150:Guanine Nucleotides; D010455:Peptides; D013873:Thionucleotides; D010566:Virulence Factors, Bordetella; C015462:polyarginine; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D006160:Guanosine Triphosphate; D007770:L-Lactate Dehydrogenase; D037342:Pertussis Toxin; D019204:GTP-Binding Proteins; D002118:Calcium