[Expression of the nif and ntr genes of Klebsiella pneumoniae in Rhodobacter sphaeroides cells].
The genes glnA, ntr, nif or their promoters from Klebsiella pneumoniae cloned on the vectors, based on the plasmid RSF1010, were introduced into Rhodobacter sphaeroides cells. It was found that K. pneumoniae genes glnA, nifB, nifE, nifL and nifH are not expressed in R. sphaeroides. Neither was the glnA gene from cyanobacterium Anabaena 7120 expressed in R. sphaeroides. No functional activity of K. pneumoniae product of ntrA gene which is expressed from its own promoter, and the product of the gene nifA which is expressed from the constitutive promoter of the kanamycin resistance gene of the transposon Tn903, was detected. The implications of these findings are discussed.
D003227:Conjugation, Genetic; D000458:Cyanobacteria / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D005786:Gene Expression Regulation; D005798:Genes, Bacterial; D005822:Genetic Vectors; D007711:Klebsiella pneumoniae / Q000235:genetics*; D009586:Nitrogen Fixation / Q000235:genetics*; D009591:Nitrogenase / Q000235:genetics; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012242:Rhodobacter sphaeroides / Q000201:enzymology / Q000235:genetics*; D014169:Transformation, Bacterial
D004269:DNA, Bacterial; D009591:Nitrogenase
Issledovanie ékspressii nif- i ntr-genov Klebsiella pneumoniae v kletkakh Rhodobacter sphaeroides.