Generation of activated killer-like cells by OK-432-mediated production of interleukin 1, interleukin 2, and interferon-gamma in vitro.
Human peripheral blood mononuclear (PBM) cells incubated for 3 or more days in medium containing streptococcal preparation OK-432 developed toxicity to natural killer-resistant tumor cell lines. The generation of cytotoxic cells by OK-432 was potentiated by exogenous lymphokines such as interleukin 2 (IL-2) and interferon-gamma (IFN-gamma), the former being much more effective than the latter, and was blocked by antibodies to these cytokines such as interleukin 1 (IL-1), IL-2, and IFN-gamma. Although monocyte-depleted PBM cells were barely responsive to OK-432, they gave rise to activated killer (AK) cells after being incubated in medium with both IL-2 and IFN-gamma. Their generation was augmented by exogenous IL-1 but not by OK-432. These data indicate that AK-like cells are generated from human PBM cells through mediation of IL-1, IL-2, and IFN-gamma produced by OK-432 in vitro.
D001688:Biological Products / Q000494:pharmacology*; D003602:Cytotoxicity, Immunologic; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis*; D007375:Interleukin-1 / Q000096:biosynthesis*; D007376:Interleukin-2 / Q000096:biosynthesis*; D007694:Killer Cells, Natural / Q000276:immunology* / Q000378:metabolism; D008213:Lymphocyte Activation; D010844:Picibanil / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D001688:Biological Products; D007375:Interleukin-1; D007376:Interleukin-2; D010844:Picibanil; D007371:Interferon-gamma