Comparative study of alloimmune reactions induced by leukocyte and platelet transfusions in humans: characteristic changes of activation markers, gamma interferon, and FcR blocking antibody production.
This study was undertaken to define immune responses induced by leukocyte and platelet transfusions. We offer evidence that activation by alloimmunization with intravenously administered "buffy coat" cells results in immune alterations similar to the early rejection episodes following kidney transplantation. Thus, IL-2 and HLA-DR expression increased on PBL 2 and 3 days after antigen exposure and paralleled elevations of IFN-gamma, neopterin, and beta 2 microglobulin levels. No significant changes were detected in the number of T-cell subpopulations. Alloimmunization by purified platelets alone that express only class I MHC antigens failed to induce the above alteration, even after repeated exposure. Repeated alloimmunization with "buffy coat" cells or isolated platelets stimulated a progressive increase up to 100% blocking activity on EA rosette formation. We conclude that the expression of activation markers on PBL and the production of IFN gamma and neopterin reflect the early phase of alloimmune response induced by leukocytes. This type of alloactivation is not a prerequisite for the development of FcR-blocking antibody, which is produced after pure platelet transfusion as well.
D001667:Binding, Competitive; D001792:Blood Platelets / Q000276:immunology; D001803:Blood Transfusion; D003602:Cytotoxicity, Immunologic; D006801:Humans; D007114:Immunization; D007371:Interferon-gamma / Q000096:biosynthesis; D007518:Isoantibodies / Q000096:biosynthesis*; D007519:Isoantigens / Q000008:administration & dosage; D017708:Leukocyte Transfusion; D007962:Leukocytes / Q000276:immunology; D008213:Lymphocyte Activation; D008297:Male; D017713:Platelet Transfusion; D011961:Receptors, Fc / Q000276:immunology; D013601:T-Lymphocytes / Q000145:classification
D007518:Isoantibodies; D007519:Isoantigens; D011961:Receptors, Fc; D007371:Interferon-gamma