Inhibitors of metal catalyzed lipid peroxidation reactions inhibit mucus secretion and 15 HETE levels in canine trachea.
Inhibition of canine mucus secretion in vivo induced by arachidonic acid administration was correlated with a reduction of 15 HETE levels in canine mucus. Antioxidants and inhibitors of lipid peroxidation were effective inhibitors of both mucus secretion and 15 HETE production. This same series of inhibitors also dose dependently inhibited Fe2+ dependent oxidation of arachidonic acid in vitro as assessed by an inhibition of thiobarbituric acid reactive material and conjugated diene formation. These data argue for an involvement of reactive oxygen species and lipid peroxidation in the generation and elaboration of mucus secretion.
D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000494:pharmacology; D003676:Deferoxamine / Q000494:pharmacology; D004285:Dogs; D006893:Hydroxyeicosatetraenoic Acids / Q000032:analysis*; D007501:Iron / Q000494:pharmacology; D008054:Lipid Peroxides / Q000378:metabolism*; D009093:Mucus / Q000378:metabolism*; D014132:Trachea / Q000032:analysis / Q000187:drug effects / Q000378:metabolism*
D001095:Arachidonic Acids; D006893:Hydroxyeicosatetraenoic Acids; D008054:Lipid Peroxides; D016718:Arachidonic Acid; C025984:15-hydroxy-5,8,11,13-eicosatetraenoic acid; D007501:Iron; D003676:Deferoxamine