Continuous determination of effect of temperature on enzymic activities.
An electronic temperature controller was used to continuously change the temperature of cuvettes during enzymic assays. A microprocessor-controlled spectrophotometer continuously collected absorbance, time, and temperature data. Activation energies were calculated from Arrhenius plots of these data.
D004789:Enzyme Activation; D005959:Glucosidases / Q000378:metabolism*; D008838:Microcomputers; D013053:Spectrophotometry; D013696:Temperature; D001617:beta-Glucosidase / Q000378:metabolism*
D005959:Glucosidases; D001617:beta-Glucosidase