Preparation and characterization of monoclonal antibodies against phosphoenolpyruvate carboxylase of Escherichia coli.
Twelve hybridoma clones which secrete monoclonal antibodies (mAb) against purified phosphoenolpyruvate carboxylase [EC 4.1.1.31] from Escherichia coli K-12 were obtained. These 12 mAb were prepared from the ascites fluids of mice. Six among the 12 mAb formed precipitin lines with the enzyme on immunodiffusion. Four mAb inhibited the activity of the enzyme and 2 mAb enhanced it. Four mAb altered the sensitivity of the enzyme to allosteric effectors. Competitive enzyme-binding experiments among the 12 different mAb were also performed. The results showed that the 12 mAb can be classified into at least 8 groups.
D000494:Allosteric Regulation; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D000941:Antigens / Q000276:immunology; D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D002262:Carboxy-Lyases / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000201:enzymology*; D006825:Hybridomas / Q000276:immunology; D005779:Immunodiffusion; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010730:Phosphoenolpyruvate Carboxylase / Q000276:immunology* / Q000378:metabolism
D000911:Antibodies, Monoclonal; D000941:Antigens; D002262:Carboxy-Lyases; D010730:Phosphoenolpyruvate Carboxylase