Antisera against the 3-17 sequence of rat G alpha i recognize only a 40 kDa G-protein in brain.
To obtain antisera specific for the GTP-binding protein Gi alpha we immunized rabbits against a synthetic peptide derived from the N-terminal (3-17) sequence predicted from the rat Gi alpha cDNA clone published by Itoh et al. (1986) (Proc. Natl. Acad. Sci. USA 83, 3776-3780). Western-blot analysis of bovine brain G-proteins purified and resolved by hydrophobic chromatography and of rat striatal membranes, indicate that this antiserum does not recognize 41 kDa alpha i or 39 kDa alpha o. However, it reacts with a 40 kDa alpha-subunit. The data suggest that the sequence deduced from the rat G alpha i cDNA corresponds to a G40 alpha protein and that N-terminus directed antisera are useful tools to discriminate between two different G alpha i-like types of G-proteins present in mammalian brain.
D000595:Amino Acid Sequence; D000818:Animals; D000936:Antigen-Antibody Complex; D002417:Cattle; D002540:Cerebral Cortex / Q000032:analysis; D002852:Chromatography, Ion Exchange; D004247:DNA; D004591:Electrophoresis, Polyacrylamide Gel; D019204:GTP-Binding Proteins / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D006160:Guanosine Triphosphate / Q000031:analogs & derivatives; D006801:Humans; D007106:Immune Sera; D008969:Molecular Sequence Data; D011485:Protein Binding; D012689:Sequence Homology, Nucleic Acid; D013873:Thionucleotides
D000936:Antigen-Antibody Complex; D007106:Immune Sera; D013873:Thionucleotides; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D006160:Guanosine Triphosphate; D004247:DNA; D019204:GTP-Binding Proteins