A study of the coagulant activity of Indian green pit viper venom.
Indian green pit viper venom was studied for its coagulant activity. It was observed that the venom contained a significant amount of coagulant activity, which was similar to thrombin in its action on plasma as well as on fibrinogen. The physicochemical properties studied suggested that the venom coagulant activity lacked both platelet aggregating and factor XIII activating properties. Unlike thrombin, the activity was retained in the presence of heparin and at high temperatures. The activity was inhibited by Diisopropyl fluorophosphate and phenyl methyl sulphonyl fluoride, indicating that it was a serine esterase.
D001777:Blood Coagulation; D003018:Clot Retraction; D003435:Crotalid Venoms / Q000494:pharmacology*; D004492:Edetic Acid / Q000494:pharmacology; D006493:Heparin / Q000494:pharmacology; D007531:Isoflurophate / Q000494:pharmacology; D010664:Phenylmethylsulfonyl Fluoride / Q000494:pharmacology; D013696:Temperature; D013918:Thrombin Time
D003435:Crotalid Venoms; D007531:Isoflurophate; D010664:Phenylmethylsulfonyl Fluoride; D006493:Heparin; D004492:Edetic Acid