Immunochemical characterization of proteins from scrapie-infected hamster brain, using immunoblot analysis.
Preparations of brain plasma membrane from scrapie-infected or noninfected hamsters were extracted with a solvent and were used to inoculate rabbits. Antisera evaluated by immunoblot analysis revealed a protein of 45 kD in scrapie-infected hamster brain that had a greater signal compared with proteins of comparable relative mass in noninfected brain. This 45-kD protein was not increased in scrapie-infected mouse, sheep, or goat brain. Seemingly, the 45-kD protein may be a degradation product of glial fibrillary acidic protein.
D000818:Animals; D001923:Brain Chemistry; D001927:Brain Diseases / Q000382:microbiology*; D006224:Cricetinae; D007118:Immunoassay; D008297:Male; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000276:immunology*; D011328:Prions / Q000276:immunology*; D012608:Scrapie / Q000276:immunology*; D014764:Viral Proteins / Q000032:analysis / Q000276:immunology*
D009419:Nerve Tissue Proteins; D011328:Prions; D014764:Viral Proteins