The regulation of prostaglandins by vasoactive hormones in renal tubular and vascular smooth muscle cells.
The production of prostaglandins by rat renal tubular cells and by rat vascular smooth muscle cells (VSMC) in response to vasoactive hormones was examined. A superfusion technique was used to stimulate collagenase-dispersed renal cortical or medullary tubular cells and trypsinized rat aortic smooth muscle cells with vasoactive hormones and ANF. All cell types responded promptly to the stimuli in a dose-dependent manner. Renal tubular cells produced mainly PGE2, less PGF2 alpha and no 6-keto-PGF1 alpha, while VSMC produced exclusively 6-keto-PGF1 alpha. This production of PG was strictly dependent on the presence of extracellular Ca2+ and was not inhibited by antagonists of voltage-dependent Ca2+-channels. Angiotensin II (Ang II) was active on cortical tubular cells and VSMC. Sar1-Ala8-angiotensin II blocked this action. Arginine-vasopressin (AVP acted on medullary tubular cells and VSMC and its effect was inhibited by selective V1-antagonists. The V2-agonist dDAVP had no effect on PG production. A clear distinction between V1-receptor mediated PG release and V2-receptor mediated cAMP extrusion was observed in medullary tubular cells. Bradykinin was a weak agonist on medullary tubular cell. The synthetic (1-24) atrial natriuretic peptide did not prevent 6-keto-PGF1 alpha release induced by Ang II or AVP in VSMC nor the PGE2 release in cortical tubular cells induced by Ang II.
D000804:Angiotensin II / Q000494:pharmacology; D000818:Animals; D001127:Arginine Vasopressin / Q000494:pharmacology; D000001:Calcimycin / Q000494:pharmacology; D011464:Epoprostenol / Q000096:biosynthesis; D066298:In Vitro Techniques; D007684:Kidney Tubules / Q000187:drug effects* / Q000378:metabolism; D009131:Muscle, Smooth, Vascular / Q000187:drug effects* / Q000378:metabolism; D011453:Prostaglandins / Q000378:metabolism*; D051381:Rats; D014662:Vasoconstrictor Agents / Q000494:pharmacology*
D011453:Prostaglandins; D014662:Vasoconstrictor Agents; D000804:Angiotensin II; D001127:Arginine Vasopressin; D000001:Calcimycin; D011464:Epoprostenol