Effects of a poly(ADP-ribose) polymerase inhibitor on mutation frequencies in dividing and quiescent C3H10T1/2 cells.
The effect of 3-methoxybenzamide, an inhibitor of poly(ADP-ribose) polymerase, on N-methyl-N'-nitro-N-nitrosoguanidine-induced mutations has been examined in exponentially dividing cells where this inhibitor is a strong potentiator of cytotoxicity and in quiescent cells where the inhibitor has little or no effect on cell survival. The yield of mutants decreased in dividing cells by approximately 70% while mutation frequencies showed small but statistically significant increases in quiescent cells. These results suggest that apparent decreased mutation frequencies observed in the presence of ADP-ribosylation inhibitors are due to selective inhibition of expression of mutations in dividing cells caused by an irreversible G2 cell cycle block.
D000818:Animals; D001549:Benzamides / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004347:Drug Interactions; D007700:Kinetics; D008769:Methylnitronitrosoguanidine / Q000633:toxicity*; D051379:Mice; D008809:Mice, Inbred C3H; D009152:Mutagenicity Tests; D009153:Mutagens; D009154:Mutation; D000067856:Poly(ADP-ribose) Polymerase Inhibitors
D001549:Benzamides; D009153:Mutagens; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D008769:Methylnitronitrosoguanidine; C025159:3-methoxybenzamide