A short 5'-flanking DNA region is sufficient for developmentally correct expression of moth chorion genes in Drosophila.
Fusions with the bacterial gene for chloramphenicol acetyltransferase followed by P-element-mediated germ-line transformation in Drosophila have permitted localization of the DNA sequence that confers a high degree of developmental specificity on a pair of silkmoth eggshell (chorion) genes. The short, 272-base-pair, 5'-flanking region shared by the divergently transcribed genes is sufficient for developmentally appropriate expression when placed upstream of the chloramphenicol acetyltransferase gene, in either orientation. A highly conserved motif within that region, TCACGT, is essential for chorion-specific expression.
D000818:Animals; D012831:Bombyx / Q000235:genetics*; D002823:Chorion; D004274:DNA, Recombinant; D004331:Drosophila melanogaster / Q000235:genetics; D004527:Egg Proteins / Q000235:genetics; D005260:Female; D005786:Gene Expression Regulation; D008297:Male; D009928:Organ Specificity; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid; D012737:Sex Factors; D013997:Time Factors
D004274:DNA, Recombinant; D004527:Egg Proteins; D011993:Recombinant Fusion Proteins