title: Electro-stimulated transformation of E. coli cells pretreated by EDTA solution.
abstract: The phenomenon of transformation of E. coli cells under electric treatment has been studied. The cells of strains MH 1, HB 101 and DH 1 after EDTA treatment in an isotonic medium were transformed with DNA pBR322 by applying a single exponential pulse (E = 10 kV/cm, T = 1.5 ms) to the suspension. The maximum transformation efficiency obtained was 4 X 10(6) colonies/micrograms DNA. The maximum transformation frequency was 0.4% at a DNA concentration of 15 micrograms/ml.
mesh_terms: D004269:DNA, Bacterial / Q000235:genetics; D004492:Edetic Acid / Q000494:pharmacology*; D004560:Electricity; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics*; D010957:Plasmids; D014169:Transformation, Bacterial / Q000187:drug effects
chemical_list: D004269:DNA, Bacterial; D004492:Edetic Acid