A monoclonal anti-thromboxane B2 antibody.
A monoclonal antibody against thromboxane B2 which may be used in standard fluid phase radioimmunoassays with a detection limit of around 40 pg and a binding affinity of 1.98 X 10(9) M-1 is described. Limited crossreactivity could be observed only with structurally closely related compounds such as 2,3-dinor-thromboxane B2 (8.9%), thromboxane B1 (15.7%) and thromboxane B3 (39.7%). Detectable crossreactivity with 11-dehydro-thromboxane B2, omega-carboxy-thromboxane B2, omega-hydroxy-thromboxane B2, prostaglandins of the D-, E- and F-type as well as metabolites of prostacyclin was lacking. The monoclonal anti-thromboxane B2 antibody proved well suited for measuring the thromboxane B2 content in tissue culture supernatants as well as in human serum.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000915:Antibody Affinity; D000918:Antibody Specificity; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000494:pharmacology; D006801:Humans; D007052:Ibuprofen / Q000494:pharmacology; D007093:Imidazoles / Q000494:pharmacology; D007074:Immunoglobulin G / Q000276:immunology; D007213:Indomethacin / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D010974:Platelet Aggregation / Q000187:drug effects; D011863:Radioimmunoassay; D013928:Thromboxane A2 / Q000276:immunology / Q000502:physiology; D013929:Thromboxane B2 / Q000031:analogs & derivatives / Q000032:analysis / Q000276:immunology*
D000911:Antibodies, Monoclonal; D001095:Arachidonic Acids; D007093:Imidazoles; D007074:Immunoglobulin G; D016718:Arachidonic Acid; C039242:dazmegrel; D013929:Thromboxane B2; D013928:Thromboxane A2; C028965:2,3-dinor-thromboxane B2; D007052:Ibuprofen; D007213:Indomethacin