Demonstration of a cytotoxin from Campylobacter pylori.
In order to determine if Campylobacter pylori produces a cytotoxin, a study was performed using bacterial lysates from three clinical isolates of the organism. The lysates were cytotoxic for Chinese hamster ovary cells, as determined by a microtiter assay. The lysates were also lethal for mice after intraperitoneal injection. Loss of toxicity and lethality followed trypsinization, heating and acidification of cell-free lysates. It is concluded that the toxic factor(s) in Campylobacter pylori may be a protein.
D000818:Animals; D001427:Bacterial Toxins / Q000032:analysis / Q000096:biosynthesis* / Q000633:toxicity; D002167:Campylobacter / Q000378:metabolism* / Q000472:pathogenicity; D002169:Campylobacter Infections / Q000382:microbiology*; D002460:Cell Line; D002470:Cell Survival; D002648:Child; D003603:Cytotoxins / Q000032:analysis / Q000096:biosynthesis* / Q000633:toxicity; D005756:Gastritis / Q000382:microbiology*; D006801:Humans; D051379:Mice; D014774:Virulence
D001427:Bacterial Toxins; D003603:Cytotoxins