Differentiation of Mycobacterium thermoresistibile from Mycobacterium phlei and other rapidly growing mycobacteria.
Mycobacterium thermoresistibile is the only chromogenic rapidly growing mycobacterial species reported to cause infections in humans and animals. DNA-DNA hybridization (S1 nuclease method) showed that M. thermoresistibile formed a DNA relatedness group which was only 24 to 30% related to M. phlei. Alkaline phosphatase and beta-galactosidase differentiated M. thermoresistibile from M. phlei. Among the rapidly growing mycobacteria, the mycolic acid pattern of M. thermoresistibile was unique (alpha-, alpha'-, methoxy-and keto-mycolates). Fourteen other species of chromogenic rapidly growing mycobacteria, including M. phlei, produced different mycolic acid patterns which always included dicarboxylic mycolate. Nine species of non-chromogenic rapidly growing mycobacteria produced mycolic acid patterns devoid of ketomycolate.
D000469:Alkaline Phosphatase / Q000032:analysis; D001669:Biochemical Phenomena; D001671:Biochemistry; D003470:Culture Media; D004269:DNA, Bacterial; D009161:Mycobacterium / Q000302:isolation & purification* / Q000378:metabolism; D009168:Mycobacterium phlei / Q000032:analysis / Q000302:isolation & purification; D009171:Mycolic Acids / Q000032:analysis; D009693:Nucleic Acid Hybridization; D010405:Penicillinase / Q000032:analysis; D001616:beta-Galactosidase / Q000032:analysis; D001617:beta-Glucosidase / Q000032:analysis
D003470:Culture Media; D004269:DNA, Bacterial; D009171:Mycolic Acids; D000469:Alkaline Phosphatase; D001617:beta-Glucosidase; D001616:beta-Galactosidase; D010405:Penicillinase