title: Observed convergence of the Salmonella plate and pre-incubation assays when employing varying levels of S9.
abstract: Since the publication of the original Salmonella mutagenicity test protocol in 1975, a number of investigators have proposed that the addition of a pre-incubation step to the normal protocol increases the sensitivity of the assay. Data presented here for 4-dimethylaminoazobenzene and the more potent carcinogenic analogue 6-dimethylaminophenylazobenzothiazole show that simply varying the amount of S9 present in the plate assay can increase the sensitivity of the test as much as the addition of the pre-incubation step does.
mesh_terms: D000818:Animals; D052160:Benzothiazoles; D001711:Biotransformation; D066298:In Vitro Techniques; D009152:Mutagenicity Tests / Q000379:methods*; D009153:Mutagens / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012486:Salmonella typhimurium / Q000187:drug effects; D013844:Thiazoles / Q000378:metabolism / Q000633:toxicity; D013997:Time Factors; D004124:p-Dimethylaminoazobenzene / Q000031:analogs & derivatives / Q000378:metabolism / Q000633:toxicity
chemical_list: D052160:Benzothiazoles; D009153:Mutagens; D013844:Thiazoles; C012103:N,N-dimethyl-4-(6-benzothiazolylazo)aniline; D004124:p-Dimethylaminoazobenzene