Automated analysis of mitomycin C in body fluids by high-performance liquid chromatography with on-line sample pre-treatment.
A fully automated liquid chromatographic system for the bioanalysis of mitomycin C has been described. The isolation of the analyte from the biological matrix (plasma, ascites and urine) is performed using a continuous-flow system equipped with a dialysis membrane in order to remove proteins. The samples are concentrated on a reversed-phase pre-column and subsequently introduced on to a reversed-phase analytical column by applying column-switching techniques. The drug is detected by absorbance measurements at 360 nm. Using the described system up to 100 samples a day can be analysed with determination limits of the order of 1 ng/ml, with a linear dynamic range of at least three decades for plasma and urine samples. The procedure was applied to pharmacokinetic studies of ovarian cancer patients treated intraperitoneally with mitomycin C.
D001202:Ascitic Fluid / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003956:Dialysis; D005260:Female; D006801:Humans; D008832:Microchemistry; D016685:Mitomycin; D008937:Mitomycins / Q000032:analysis* / Q000493:pharmacokinetics; D010051:Ovarian Neoplasms / Q000378:metabolism; D013048:Specimen Handling
D008937:Mitomycins; D016685:Mitomycin