Isolation and partial characterization of IgG-like immunoglobulins from frog (Rana ridibunda Pall.) and tortoise (Testudo graeca Pall.) serum.
1. By successive application of gel-filtration and ion-exchange chromatography IgG fraction have been isolated from frog (Rana ridibunda Pall.) and tortoise (Testudo graeca Pall.) sera. 2. The homogeneity of the fractions has been confirmed and a similar electrophoretic mobility has been revealed by polyacrylamide gel electrophoresis and immunoelectrophoresis. 3. In rabbits polyclonal antibodies giving specific reaction with the corresponding IgG immunoglobulin have been raised.
D000818:Animals; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D007074:Immunoglobulin G / Q000032:analysis* / Q000302:isolation & purification; D011895:Rana ridibunda / Q000097:blood / Q000276:immunology*; D011898:Ranidae / Q000276:immunology*; D014426:Turtles / Q000097:blood / Q000276:immunology*
D007074:Immunoglobulin G