Isolation and characterization of ras-transfected BALB/3T3 clone showing morphological transformation by 12-O-tetradecanoyl-phorbol-13-acetate.
The transformation frequency of mouse BALB/3T3 cells was significantly enhanced after transfection with an activated ras oncogene (v-Ha-ras) followed by treatment with a tumor promoter, 12-O-tetradecanoyl-phorbol-13-acetate (TPA), suggesting that the ras oncogene acted as an initiator in two-stage carcinogenesis. A cell clone (Bhas42) containing the ras oncogene was isolated from the ras-transfected BALB/3T3 cells. Bhas42 cells were flat and showed contact inhibition, but the addition of TPA to quiescent Bhas42 cultures resulted in a dramatic change of cell morphology to spindle shape, doubling of the cell population, and increased DNA synthesis.
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002471:Cell Transformation, Neoplastic / Q000139:chemically induced*; D002999:Clone Cells; D004247:DNA / Q000096:biosynthesis / Q000235:genetics; D011905:Genes, ras / Q000187:drug effects*; D051379:Mice; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D013755:Tetradecanoylphorbol Acetate / Q000633:toxicity*; D014162:Transfection; D014407:Tumor Cells, Cultured / Q000187:drug effects
D004247:DNA; D013755:Tetradecanoylphorbol Acetate