Light scatter and cell surface properties of murine megakaryocyte progenitor cells.
The characteristics with respect to light scatter and cell surface properties of megakaryocyte colony-forming cells (CFU-Meg), growing in serum-free agar cultures, have been determined. Mouse bone marrow cells were fractionated using a light-activated cell sorter (FACS) either on the basis of light scatter or after staining with a fluorescein isothiocyanate (FITC)-labeled lectin or monoclonal antibody. After staining and sorting, the recovery of CFU-Meg was about 50%. In the unsorted controls 29 +/- 2 CFU-Meg/10(5) cells were observed. The forward (FLS) and perpendicular (PLS) light scatter intensities of CFU-Meg were very similar to those of spleen colony-forming cells (CFU-S) and early progenitor cells. The average diameter of the CFU-Meg as determined by the FLS intensity profile was about 7.5 microns. Wheat germ agglutinin binding of CFU-Meg was high, indicating a high sialic acid content of the cell surface. Expression of the differentiation antigen Pgp-1 on the CFU-Meg as determined by indirect immunofluorescence with the rat monoclonal antibody I42/5.1 was moderate. Pgp-1 expression was lower than the Pgp-1 expression of granulocyte/macrophage progenitors (CFU-C) or granulocytes. It is concluded that the light scatter and cell surface properties of CFU-Meg are similar to those of CFU-S and early committed progenitors.
D000818:Animals; D000943:Antigens, Differentiation / Q000276:immunology; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins / Q000378:metabolism; D008027:Light; D008297:Male; D008533:Megakaryocytes / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D008815:Mice, Inbred Strains; D012542:Scattering, Radiation; D013234:Stem Cells / Q000276:immunology / Q000378:metabolism*; D013499:Surface Properties; D013861:Thiocyanates / Q000378:metabolism; D014909:Wheat Germ Agglutinins / Q000378:metabolism
D000943:Antigens, Differentiation; D005452:Fluoresceins; D013861:Thiocyanates; D014909:Wheat Germ Agglutinins; D016650:Fluorescein-5-isothiocyanate