title: Characterization of primary translation products from ovine and rat salivary gland mRNAs.
abstract: Ovine and rat salivary gland mRNAs have been prepared and their translation products characterized. A 60 kD translation product from ovine submaxillary and sublingual gland mRNAs is identical in mass to the ovine apomucin. Two additional ovine translation products, 25 and 40 kD, are specific to mucin-producing salivary glands. Four rat mRNA translation products are encoded by mucin-producing salivary glands (38, 44, 67, 69 kD). These polypeptides were not detected in the parotid gland mRNAs, a serous gland. Each of these products has a high level of [3H]serine incorporation, a characteristic of mucins. The nature of these products suggests that they are mucins or mucin-like and that their molecular weights should approximate that of the corresponding apomucins.
mesh_terms: D000818:Animals; D002474:Cell-Free System; D005752:Gastric Mucins; D008970:Molecular Weight; D009077:Mucins / Q000096:biosynthesis*; D010306:Parotid Gland / Q000032:analysis; D010452:Peptide Biosynthesis; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D012469:Salivary Glands / Q000032:analysis*; D012694:Serine / Q000378:metabolism; D012756:Sheep; D013361:Sublingual Gland / Q000032:analysis; D013363:Submandibular Gland / Q000032:analysis
chemical_list: D005752:Gastric Mucins; D009077:Mucins; D012333:RNA, Messenger; C053366:apomucin; D012694:Serine