Botulinum toxin-induced ADP-ribosylation and inhibition of exocytosis are unrelated events.
The hypothesis that inhibition of secretion by botulinum neurotoxin type D occurs by an intracellular process involving ADP-ribosylation has been directly tested by measuring both the extent of inhibition of secretion and of ADP-ribosylation in the same cells. Although the inhibitory effect of unpurified toxin closely parallels intracellular ribosylation, the two events are clearly unrelated, as using purified D and C3 toxins together with their antibodies, each of these events can be either stimulated or inhibited independently of each other.
D000246:Adenosine Diphosphate Ribose / Q000378:metabolism*; D000313:Adrenal Medulla / Q000378:metabolism*; D000818:Animals; D000906:Antibodies / Q000502:physiology; D001905:Botulinum Toxins / Q000494:pharmacology*; D002118:Calcium / Q000494:pharmacology; D002395:Catecholamines / Q000378:metabolism*; D002417:Cattle; D002478:Cells, Cultured; D002838:Chromaffin System / Q000378:metabolism*; D005089:Exocytosis; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism
D000906:Antibodies; D002395:Catecholamines; D000246:Adenosine Diphosphate Ribose; D011065:Poly(ADP-ribose) Polymerases; D001905:Botulinum Toxins; D002118:Calcium