title: Both androgen and glucocorticoid induce identical secretory proteins in serum-free culture of Shionogi carcinoma 115 cells.
abstract: The effects of androgen or glucocorticoid on the induction of secretory proteins in SC-3 cells (a cloned cell line from Shionogi carcinoma 115) were examined in a serum-free medium [Ham's F-12: Eagle's minimum essential medium (1:1, v/v) containing 0.1% bovine serum albumin]. Through analysis of [35S]methionine-labeled proteins by one-dimensional (sodium dodecyl sulfate polyacrylamide) gel electrophoresis, we successfully demonstrated the production of a testosterone (10(-9)-10(-6) M)- or dexamethasone (10(-8) M)-induced secretory protein with a molecular weight of 24,000 in SC-3 cells. However, 17 beta-estradiol (10(-8) M) or progesterone (10(-8) M) had no such effect. The production of the secretory 24K protein increased slightly within 2 hr after the addition of 10(-8) M testosterone and increased further thereafter. The addition of tunicamycin to the incubation mixture with [35S]methionine resulted in the identification of a testosterone- or dexamethasone-induced secretory protein with a molecular weight of 20,000. By the use of two-dimensional gel electrophoresis, we could demonstrate that both testosterone (10(-8) M) and dexamethasone (10(-8) M) added to the serum-free medium stimulate the secretion from SC-3 cells of the same 5 proteins (30-40K, 39K, 30K, 27K proteins and the secretory 24K glycoprotein). The present findings show that physiological concentrations of both androgen and glucocorticoid induce the secretion of the same 5 proteins from SC-3 cells.
mesh_terms: D000818:Animals; D002460:Cell Line; D002999:Clone Cells; D003907:Dexamethasone / Q000494:pharmacology*; D007700:Kinetics; D008297:Male; D008325:Mammary Neoplasms, Experimental / Q000378:metabolism*; D008715:Methionine / Q000378:metabolism; D051379:Mice; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D013462:Sulfur Radioisotopes; D013739:Testosterone / Q000494:pharmacology*
chemical_list: D009363:Neoplasm Proteins; D013462:Sulfur Radioisotopes; D013739:Testosterone; D003907:Dexamethasone; D008715:Methionine