Two pairs of recombination signals are sufficient to cause immunoglobulin V-(D)-J joining.
The minimum sequence requirements for antigen receptor V-(D)-J joining were studied by constructing recombination-substrates containing synthetic recombination signals and introducing them into a recombination-competent pre-B cell line. Two sets of heptamer (CACTGTG) and nonamer (GGTTTTTGT) sequences were shown to be sufficient to cause the V-(D)-J joining, if the 12- and 23-base pair spacer rule is satisfied. A point mutation in the heptamer sequence, or a change in the combination of the two spacer lengths, drastically reduced the recombination.
D000818:Animals; D001483:Base Sequence; D007446:Chromosome Inversion; D005803:Genes, Immunoglobulin; D005822:Genetic Vectors; D006801:Humans; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D007145:Immunoglobulin kappa-Chains / Q000235:genetics; D051379:Mice; D011948:Receptors, Antigen, T-Cell / Q000235:genetics; D011995:Recombination, Genetic; D012190:Retroviridae / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D013329:Structure-Activity Relationship
D007135:Immunoglobulin Variable Region; D007145:Immunoglobulin kappa-Chains; D011948:Receptors, Antigen, T-Cell