The appearance of glycoconjugates associated with cortical granule release during mouse fertilization.
For the first time we have shown with appropriately labelled lectins that fucosyl- and sialyl-rich glycoconjugates are released into the perivitelline space of the mouse oocyte after activation by the fertilizing spermatozoon or artificial activation by the calcium ionophore A23187 or ethanol. The glycoconjugates show a punctate distribution over the oocyte surface except for the microvilli-free area overlying the second meiotic spindle from which they are absent. Their appearance in the perivitelline space is associated with the release of the cortical granule suggesting that they represent part of the cortical granule exudate. Soon after the glycoconjugates appear, they begin to aggregate. The process continues until the beginning of cytokinesis at first cleavage when a single large aggregate is found within the cleavage furrow. Most of the labelled glycoconjugates disappear by the late 2-cell stage and no evidence was found for their presence during the later preimplantation period. This technique is suitable for monitoring the kinetics of the cortical reaction in mammalian oocytes and investigating the importance of the glycoconjugates in early preimplantation period.
D000818:Animals; D060829:Arthropod Proteins; D005306:Fertilization; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins / Q000378:metabolism; D006001:Glycoconjugates / Q000378:metabolism*; D037102:Lectins / Q000378:metabolism; D051379:Mice; D008856:Microscopy, Fluorescence; D009865:Oocytes / Q000378:metabolism*; D037121:Plant Lectins; D013861:Thiocyanates / Q000378:metabolism
D060829:Arthropod Proteins; D005452:Fluoresceins; D006001:Glycoconjugates; D037102:Lectins; D037121:Plant Lectins; D013861:Thiocyanates; C036850:Ulex europaeus lectins; C027112:fucose-binding lectin; D016650:Fluorescein-5-isothiocyanate