Biological effects of a rat monoclonal anti-mouse IFN-gamma antibody produced by in vitro immunization.
This paper describes the biological effect of monoclonal antibodies to murine IFN-gamma produced by in vitro immunization with only several nanograms of rIFN-gamma. Four mAbs binding to rIFN-gamma were selected. mAb U7 was characterized in detail and shown to bind specifically to rIFN-gamma in a Western blot and to specifically inhibit the antiviral effect of rIFN-gamma and natural IFN-gamma. The activities of IFN-alpha, beta and IL2 were not affected. The M phi activating effect of both rIFN-gamma and natural IFN-gamma was also inhibited by mAb U7. Thus, we showed that it is possible to produce specific mAbs with very small amounts of cytokines by in vitro immunization.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D003588:Cytopathogenic Effect, Viral; D006825:Hybridomas / Q000276:immunology; D007114:Immunization; D066298:In Vitro Techniques; D007371:Interferon-gamma / Q000037:antagonists & inhibitors / Q000276:immunology*; D008262:Macrophage Activation; D051379:Mice; D051381:Rats; D014529:Uridine / Q000378:metabolism
D000911:Antibodies, Monoclonal; D007371:Interferon-gamma; D014529:Uridine