[Structural changes of the chromatin DNA in situ in partial deproteinization of DNP by a 0.6 M NaCl solution. A polarization-fluorescence microscopic study].
By means of polarization fluorescence microscopy changes in polarization degree of fluorescence of DNA-bound of Acridine orange in situ were observed. A marked decrease in polarization degree of chromatin fluorescence was found after the treatment of cell nuclei with 0.6 M NaCl. It shows that the removal of histone H1 from the chromatin results in destabilization of its structure. The data obtained show that the measurements of polarization degree of fluorescence allow to obtain relevant information about structural changes in DNA chromatin.
D000165:Acridine Orange / Q000378:metabolism; D000818:Animals; D002843:Chromatin / Q000032:analysis / Q000187:drug effects* / Q000378:metabolism; D004247:DNA / Q000032:analysis / Q000187:drug effects* / Q000378:metabolism; D003852:Deoxyribonucleoproteins / Q000032:analysis*; D005454:Fluorescence Polarization; D006657:Histones / Q000302:isolation & purification; D008856:Microscopy, Fluorescence; D012965:Sodium Chloride / Q000494:pharmacology*; D012996:Solutions; D013769:Tetrahymena pyriformis
D002843:Chromatin; D003852:Deoxyribonucleoproteins; D006657:Histones; D012996:Solutions; D012965:Sodium Chloride; D004247:DNA; D000165:Acridine Orange
Strukturnye izmeneniia DNK khromatina in situ pri chastichnoĭ deproteinizatsii DNP 0.6 M rastvotom NaCl. Poliarizatsionno-fluorestsentnoe mikroskopicheskoe issledovanie.