Purification and characterization of an iron stress-induced chlorophyll-protein from the cyanobacterium Anacystis nidulans R2.
An Anacystis nidulans R2 chlorophyll-protein associated with Photosystem II in iron-stressed cells (Pakrasi, H.B., Riethmann, H.C. and Sherman, L.A. (1985) Proc. Natl. Acad. Sci. USA 82, 6903-6907) has been biochemically purified and characterized. Anion exchange chromatography of dodecyl-beta-D-maltoside-solubilized membranes from iron-deficient cells was used to recover this chlorophyll-protein (termed CPVI-4) in high yield and in a relatively native state. CPVI-4 has a room temperature absorption maximum at 671 nm, a 77 K chlorophyll fluorescence peak at 681 nm, and contains polypeptides of 36, 34 and 12 kDa. The 36 and 34 kDa polypeptides are associated with chlorophyll on mildly denaturing acrylamide gels of purified CPVI-4, although only the 34 kDa protein is immunoreactive with antisera elicited against the gel-purified chlorophyll-protein. Immunoblotting experiments with dodecyl-beta-D-maltoside-solubilized membrane fractions and purified CPVI-4 indicate that CPVI-4 does not contain previously identified Photosystem II core proteins. CPVI-4 likely functions as a light-harvesting antenna complex in iron-starved cells (where phycobilisomes are absent or diminished) and, in addition, may contribute chlorophyll to the reaction center complexes during their assembly in the early stages of recovery from iron stress.
D002734:Chlorophyll / Q000302:isolation & purification*; D002845:Chromatography; D000458:Cyanobacteria / Q000032:analysis* / Q000187:drug effects; D004591:Electrophoresis, Polyacrylamide Gel; D005960:Glucosides; D007118:Immunoassay; D007501:Iron / Q000494:pharmacology*; D045342:Light-Harvesting Protein Complexes; D008970:Molecular Weight; D010455:Peptides / Q000032:analysis; D045322:Photosynthetic Reaction Center Complex Proteins; D045332:Photosystem II Protein Complex; D045524:Phycobilisomes; D010940:Plant Proteins / Q000302:isolation & purification*; D013053:Spectrophotometry
D005960:Glucosides; D045342:Light-Harvesting Protein Complexes; D010455:Peptides; D045322:Photosynthetic Reaction Center Complex Proteins; D045332:Photosystem II Protein Complex; D045524:Phycobilisomes; D010940:Plant Proteins; D002734:Chlorophyll; C040358:dodecyl maltoside; D007501:Iron