[Expression of beta-galactosidase in recombinant nonintegrated plasmids in evaluating the functional activity of vaccinia virus promoters].
The recombinant plasmids pVL1 and pVL2 were constructed for insertion and expression of alien genetic information in HindIII-F fragment of vaccinia virus DNA under the control of the strong early-late promoter of the protein 7.5. The late promoter of the main late protein 11K of vaccinia virus was cloned. These as well as other vector plasmids have been used to express the procaryotic beta-galactosidase gene. Functional activity of the genetic engineering constructions was estimated by transitory expression of beta-galactosidase after plasmid DNA transfection into the chicken fibroblasts embryo culture infected with vaccinia virus. The promoters of the genes for 7.5K and 11K proteins permitted the high level of beta-galactosidase expression. Using of the early promoter of the central part of HindIII-F fragment DNA from vaccinia virus was less efficient for expression of the enzyme.
D005696:Galactosidases / Q000235:genetics*; D005786:Gene Expression Regulation; D005798:Genes, Bacterial; D005814:Genes, Viral; D005822:Genetic Vectors; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011995:Recombination, Genetic; D014616:Vaccinia virus / Q000235:genetics*; D001616:beta-Galactosidase / Q000235:genetics*
D005696:Galactosidases; D001616:beta-Galactosidase
Ispol'zovanie ékspressii beta-galaktozidazy rekombinantnymi neintegrirovannymi plazmidami dlia otsenki funktsional'noĭ aktivnosti promotorov virusa ospovaktsiny.