[Immunohistochemical labeling of immunoglobulin antigens in semithin sections of epoxy resin-embedded tissue using the gold-silver technic].
The silver nitrate variant of the immunohistochemical gold-silver technique is applied to label L-chains within immunoglobulin containing cells in semithin sections of epoxy resin embedded human tonsils. Different possibilities of tissue fixation and of reactivation of antigens are tested. The best results are obtained by paraformaldehyde-sublimate fixation of tissue and potassium iodide processing of sections. This method allows a good preservation of both tissue and antigens and a sensitive labelling of the immunoglobulin antigens by means of the gold-silver technique.
D000941:Antigens / Q000032:analysis*; D004852:Epoxy Compounds; D005404:Fixatives; D005557:Formaldehyde; D006801:Humans; D007136:Immunoglobulins / Q000276:immunology*; D007150:Immunohistochemistry / Q000379:methods*; D014066:Palatine Tonsil / Q000276:immunology*; D011108:Polymers; D011193:Potassium Iodide; D012835:Silver Nitrate
D000941:Antigens; D004852:Epoxy Compounds; D005404:Fixatives; D007136:Immunoglobulins; D011108:Polymers; D011193:Potassium Iodide; D005557:Formaldehyde; D012835:Silver Nitrate; C003043:paraform
Immunhistochemische Markierung von Immunglobulin-Antigenen an Semidünnschnitten Epoxidharz-eingebetteten Gewebes mit der Gold-Silber-Technik.