title: Production and partial characterization of elastase of Bacillus subtilis isolated from the cervices of human females.
abstract: Conditions are described for the production of extracellular elastase by Bacillus subtilis. The yield of enzyme was maximum in shake-cultures grown in Syncase medium at 37 degrees C and was stable in culture supernatants. The enzyme, purified by ammonium sulphate precipitation and Sephadex G-75 gel filtration, showed a molecular weight of 25,000 and activity between pH 6.0 and 9.5, with an optimum of 9.0 in Tris-maleate buffer. Elastinolytic activity was maximum in glycine-NaOH buffer and minimum in phosphate buffer. Enzyme activity was adversely affected by temperature greater than or equal to 40 degrees C.
mesh_terms: D001412:Bacillus subtilis / Q000201:enzymology*; D002584:Cervix Uteri / Q000382:microbiology*; D011232:Chemical Precipitation; D002850:Chromatography, Gel; D003124:Colorimetry; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006358:Hot Temperature; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D010196:Pancreatic Elastase / Q000096:biosynthesis* / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D010196:Pancreatic Elastase