Ionic conditions for the cleavage of the tRNA-like structure of turnip yellow mosaic virus by the catalytic RNA of RNase P.
The 3'-end of the RNA genome of turnip yellow mosaic virus can form a pseudoknotted tRNA-like structure that can be recognized by several tRNA-specific enzymes. We have found that the catalytic RNA component of Bacillus subtilis RNase P can cleave this structure in unusually low ionic strength buffers at a site analogous to the 5'-end of an aminoacyl stem of a tRNA. Most other precursors can only be processed under low ionic strength conditions if the RNase P holoenzyme is used; processing by the catalytic RNA component alone requires a higher ionic strength buffer. The cleavage of the turnip yellow mosaic virus tRNA-like structure demonstrates the importance of the substrate in determining the optimal buffer conditions for this reaction and also shows that high ionic strength buffers are not always necessary for cleavage by the catalytic RNA.
D000643:Ammonium Chloride / Q000494:pharmacology; D001412:Bacillus subtilis / Q000201:enzymology; D001483:Base Sequence; D002021:Buffers; D004591:Electrophoresis, Polyacrylamide Gel; D004722:Endoribonucleases / Q000378:metabolism*; D008274:Magnesium / Q000494:pharmacology; D015636:Magnesium Chloride; D008969:Molecular Sequence Data; D009029:Mosaic Viruses / Q000235:genetics*; D009690:Nucleic Acid Conformation; D009994:Osmolar Concentration; D016337:RNA, Catalytic; D012335:RNA, Ribosomal / Q000378:metabolism*; D012343:RNA, Transfer / Q000378:metabolism*; D012367:RNA, Viral / Q000378:metabolism*; D043262:Ribonuclease P
D002021:Buffers; D016337:RNA, Catalytic; D012335:RNA, Ribosomal; D012367:RNA, Viral; D000643:Ammonium Chloride; D015636:Magnesium Chloride; D012343:RNA, Transfer; D004722:Endoribonucleases; D043262:Ribonuclease P; D008274:Magnesium