Sperm enzyme activity analysis of individual sperm for detection of heritable mutations in mammals.
Male mice were injected i.p. with 2.5 mg/kg mitomycin C, 100 mg/kg ethyl nitrosourea or saline and mated with untreated virgin females five weeks later. Sperm from 64 of the F1 male progeny were analyzed histochemically for acrosin, succinic dehydrogenase and alpha-glycerophosphate dehydrogenase activity. The frequency of F1 males with sub-normal sperm enzyme activity was significantly higher among progeny from treated males than in controls. These results show that analysis of sperm enzyme activity in F1 males is a practical method for detection of transmitted mutations induced in a treated parent.
D000176:Acrosin / Q000378:metabolism; D000818:Animals; D005038:Ethylnitrosourea / Q000494:pharmacology; D005993:Glycerolphosphate Dehydrogenase / Q000378:metabolism; D008297:Male; D051379:Mice; D016685:Mitomycin; D008937:Mitomycins / Q000494:pharmacology; D009152:Mutagenicity Tests; D013094:Spermatozoa / Q000201:enzymology*; D013385:Succinate Dehydrogenase / Q000378:metabolism
D008937:Mitomycins; D016685:Mitomycin; D005993:Glycerolphosphate Dehydrogenase; D013385:Succinate Dehydrogenase; D000176:Acrosin; D005038:Ethylnitrosourea