Radioactive labeling techniques for the identification of G antigen in the human Rh blood group system.
The G antigen is one of the erythrocyte membrane Rh antigens. The amount of Rh antigen present on the red blood cell is about 10(-15) g and radioactive labeling of membrane proteins is a useful method for its identification and characterization. In this paper, we compare 4 labeling techniques. Using a human monoclonal anti-Rh(G) antibody and an immunofixation technique, we located the G antigen on a polypeptide of an average molecular weight of 28,000 Da.
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex / Q000032:analysis; D004910:Erythrocyte Membrane / Q000276:immunology; D006801:Humans; D007457:Iodine Radioisotopes; D008970:Molecular Weight; D012204:Rh-Hr Blood-Group System / Q000032:analysis*
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D007457:Iodine Radioisotopes; D012204:Rh-Hr Blood-Group System