[Flow cytometry DNA analysis of a gastric cancer before and after intra-arterial anticancer chemotherapy--report of a case].
Flow cytometric DNA analysis has been performed on tumor tissue obtained by endoscopic biopsy before and after intraarterial infusion of ADM and 5Fu. The patient was a 74-year-old woman with a gastric cancer with an involvement of a para-aortic and cervical lymph nodes. Histological examination revealed a poorly differentiated adenocarcinoma, and flow cytometric DNA analysis disclosed that the DNA ploidy (DNA index) did not differ before and after intraarterial infusion of ADM and 5Fu. The fraction of the tumor cells in the S + G2.M phase of the cell cycle however, decreased from 56% to 44% with anticancer chemotherapy. A flow cytometric DNA analysis, using biopsied samples of tumor tissue, was found to be a useful tool for monitoring the effect of anti-cancer chemotherapy for solid tumors.
D000230:Adenocarcinoma / Q000032:analysis / Q000188:drug therapy* / Q000473:pathology; D000368:Aged; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000627:therapeutic use*; D004273:DNA, Neoplasm / Q000032:analysis*; D004317:Doxorubicin / Q000008:administration & dosage; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007261:Infusions, Intra-Arterial; D007399:Interphase; D016685:Mitomycin; D008937:Mitomycins / Q000008:administration & dosage; D013274:Stomach Neoplasms / Q000032:analysis / Q000188:drug therapy* / Q000473:pathology
D004273:DNA, Neoplasm; D008937:Mitomycins; D016685:Mitomycin; D004317:Doxorubicin