Differential induction of H-2K versus H-2D class I major histocompatibility antigens by recombinant gamma interferon. Lack of Kk augmentation in a leukemia virus-induced tumor is due to a cis-dominant effect.
T-T tumor hybrids were constructed between the AKR SL3 thymoma and an H-2-distinguishable thymoma cell line. Hybrids were stimulated with IFN-gamma to determine whether the differential augmentation of H-2D vs. H-2K class I antigen expression by AKR SL3 in response to IFN-gamma was due to effects cis or trans to the noninducible Kk gene. For each of a large number of hybrids tested, the expression of H-2Db, Kb, and Dk, but not Kk, was substantially enhanced by murine rIFN-gamma. These results suggested that the lack of induction of the Kk gene was due to an alteration cis to Kk rather than to the presence or absence of K region-specific, trans-acting negative or positive factors, respectively.
D000406:AKR murine leukemia virus; D000818:Animals; D000951:Antigens, Neoplasm / Q000096:biosynthesis* / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects; D006183:H-2 Antigens / Q000096:biosynthesis* / Q000235:genetics; D064532:Histocompatibility Antigen H-2D; D007371:Interferon-gamma / Q000494:pharmacology*; D007942:Leukemia, Experimental / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D011994:Recombinant Proteins / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000276:immunology
D000951:Antigens, Neoplasm; D006183:H-2 Antigens; C024755:H-2K(K) antigen; D064532:Histocompatibility Antigen H-2D; D011994:Recombinant Proteins; D007371:Interferon-gamma