Is astrocyte laminin involved in axon guidance in the mammalian CNS?
This paper provides evidence for the expression of laminin on glia in correlation with axon elongation and nerve pathway formation during embryonic development of the mouse optic nerve and other parts of the central nervous system (CNS). We show that punctate deposits of laminin on immature glial cells precede the entrance of the first optic axons into the nerve, and remain in close association with growing axons. Furthermore, we show that in one particular region of the optic pathway that the retinal ganglion cell axons avoid in normal animals (i.e., the pigmented area of the distal nerve) the punctate laminin matrix is missing. As the optic nerve matures punctate laminin deposits disappear, and laminin is reduced in the astroglial cytoplasm. The close correlation of the punctate form of laminin with early axonal growth is true not only in the optic nerve but also in some other parts of the CNS. We demonstrate such punctate laminin deposits in a model of astrocyte-induced regeneration of the corpus callosum in acallosal mice (G. Smith, R. Miller, and J. Silver, 1986, J. Comp. Neurol. 251, 23-43), and in glia associated with several normal developing axon trajectories, such as the corpus callosum, fornix, and pathways in the embryonic hindbrain. In all of these regions punctate laminin deposits are found on astroglia that are associated with early growing axons. Our results indicate that the punctate form of laminin, produced by astrocytes, may be an important factor involved in axon elongation and nerve pathway formation in the mammalian CNS.
D000818:Animals; D001253:Astrocytes / Q000502:physiology*; D001369:Axons / Q000502:physiology*; D001921:Brain / Q000196:embryology; D002465:Cell Movement; D002478:Cells, Cultured; D003094:Collagen / Q000502:physiology; D005353:Fibronectins / Q000502:physiology; D005455:Fluorescent Antibody Technique; D005904:Glial Fibrillary Acidic Protein / Q000502:physiology; D007381:Intermediate Filament Proteins / Q000502:physiology; D007797:Laminin / Q000502:physiology*; D051379:Mice; D016900:Neurofilament Proteins; D009900:Optic Nerve / Q000196:embryology*
D005353:Fibronectins; D005904:Glial Fibrillary Acidic Protein; D007381:Intermediate Filament Proteins; D007797:Laminin; D016900:Neurofilament Proteins; D003094:Collagen