Correlation of interleukin-2 receptor expression with tissue-specific growth of an interleukin-3-dependent autocrine leukemia.
An autocrine leukemia (FDC-P1-IL3) has been developed using a retroviral vector containing the interleukin-3 (IL-3) gene to transfect the IL-3-dependent cell line FDC-P1. When leukemia cells were reisolated from experimental animals, it was found that levels of interleukin-2 (IL-2) receptor (IL-2R) expression were greater on cells isolated from the lymph node than on cells isolated from the spleen. Cloned sublines of FDC-P1-IL3 were selected by flow microfluorometry for high or low levels of IL-2R expression. Those clones that expressed high levels of IL-2R grew preferentially in the lymph node. Although IL-2 is not mitogenic for FDC-P1 cells and does not increase the rate of growth of FDC-P1-IL3 cells in vitro, the cloning efficiency of FDC-P1-IL3 is increased fourfold in the presence of IL-2. These observations suggest that the IL-2R on FDC-P1-IL3 cells plays an important role in modulating the growth of this leukemia in sites that contain high levels of IL-2.
D000818:Animals; D002460:Cell Line; D002999:Clone Cells; D005434:Flow Cytometry; D005796:Genes; D005822:Genetic Vectors; D007376:Interleukin-2 / Q000378:metabolism*; D007377:Interleukin-3 / Q000235:genetics*; D007942:Leukemia, Experimental / Q000276:immunology*; D007951:Leukemia, Myeloid / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D011971:Receptors, Immunologic / Q000235:genetics*; D015375:Receptors, Interleukin-2; D012190:Retroviridae / Q000235:genetics*; D014162:Transfection
D007376:Interleukin-2; D007377:Interleukin-3; D011971:Receptors, Immunologic; D015375:Receptors, Interleukin-2