Binding of proteins, including pp60src, to activated CH-sepharose 4B.
Activated CH-Sepharose 4B and protein A Sepharose CL-4B can bind, selectively and non-specifically, polypeptides from chick embryo cells. The major polypeptides bound have apparent molecular masses of 57-60 kDa and 47-49 kDa and cannot be eluted by extensive washing with buffers containing detergents. One of the 57-60 kDa polypeptides was identified by immunoblotting as the transforming protein of Rous Sarcoma Virus (RSV), pp60src. This polypeptide could be removed from the solid phase immunoabsorbent with 60% dimethylsulfoxide, but not with 2% SDS, 5% beta-mercaptoethanol, 1 M NaCl or 0.1% Tween 20.
D000818:Animals; D002642:Chick Embryo; D015688:Oncogene Protein pp60(v-src); D011485:Protein Binding; D011817:Rabbits; D012191:Retroviridae Proteins / Q000378:metabolism; D012685:Sepharose / Q000031:analogs & derivatives / Q000378:metabolism*; D013205:Staphylococcal Protein A / Q000378:metabolism
D012191:Retroviridae Proteins; D013205:Staphylococcal Protein A; C035771:Sepharose CL 4B; D012685:Sepharose; D015688:Oncogene Protein pp60(v-src)