title: Effects of fixative composition on the ultrastructure of oral epithelium in guinea pigs.
abstract: Keratinizing epithelium from the hard palate of guinea pigs was used to investigate effects of variations in composition of fixative solutions on tissue preservation. With 2.5% glutaraldehyde as fixative, the volume of the basal intercellular spaces increased significantly when the buffer osmolality was increased by intervals of about 100 mOsm (0.05 M, 0.10 M and 0.15 M). The intercellular space volume appeared to be insensitive to differences in concentration of the fixative within the range of 2.5-5% glutaraldehyde. The preservation of mitochondria was improved by increasing the concentration of glutaraldehyde to 5% or by adding formaldehyde as a second fixative.
mesh_terms: D000818:Animals; D002021:Buffers; D002101:Cacodylic Acid / Q000494:pharmacology; D004848:Epithelium / Q000187:drug effects / Q000648:ultrastructure; D005404:Fixatives / Q000494:pharmacology*; D005557:Formaldehyde / Q000494:pharmacology; D005976:Glutaral / Q000494:pharmacology; D006168:Guinea Pigs; D006652:Histological Techniques; D008297:Male; D009061:Mouth Mucosa / Q000187:drug effects / Q000648:ultrastructure*; D009994:Osmolar Concentration; D010159:Palate; D011108:Polymers / Q000494:pharmacology
chemical_list: D002021:Buffers; D005404:Fixatives; D011108:Polymers; D005557:Formaldehyde; D002101:Cacodylic Acid; D005976:Glutaral; C003043:paraform