Similarity of cruzin, an inhibitor of Trypanosoma cruzi neuraminidase, to high-density lipoprotein.
A specific inhibitor of the neuraminidase of the protozoan parasite Trypanosoma cruzi was isolated recently and named cruzin. It is now shown that cruzin is similar to high-density lipoprotein by amino acid homology, by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, by immunoblot analysis, and by isoelectric focusing. Cruzin purified by ion exchange chromatography and high-density lipoprotein isolated by density gradient ultracentrifugation inhibited Trypanosoma cruzi neuraminidase to the same extent. Cruzin or high-density lipoprotein restores to normal the decreased multiplication rate of Trypanosoma cruzi epimastigotes grown in a medium depleted of lipoproteins, suggesting that it may be important for survival of the parasite in nature.
D000595:Amino Acid Sequence; D000818:Animals; D000890:Anti-Infective Agents; D016632:Apolipoprotein A-I; D001054:Apolipoproteins A / Q000235:genetics / Q000276:immunology; D001798:Blood Proteins / Q000276:immunology / Q000494:pharmacology / Q000502:physiology*; D007525:Isoelectric Focusing; D008075:Lipoproteins, HDL / Q000502:physiology*; D009439:Neuraminidase / Q000037:antagonists & inhibitors; D014349:Trypanosoma cruzi / Q000201:enzymology* / Q000254:growth & development
D000890:Anti-Infective Agents; D016632:Apolipoprotein A-I; D001054:Apolipoproteins A; D001798:Blood Proteins; D008075:Lipoproteins, HDL; D009439:Neuraminidase