Radioenzymatic determination of adenosine.
Adenosine has been measured at the nanomolar level by an enzymatic radioactive assay. The nucleoside is converted into [U-14C]ribose-labeled inosine via the following reactions: adenosine + H2O----adenine + ribose (adenosine nucleosidase); adenine + [U-14C]ribose 1-phosphate in equilibrium with T[U-14C]ribose-adenosine + Pi (adenosine phosphorylase); [U-14C]ribose-adenosine + H2O----[U-14C]ribose-inosine + NH3 (adenosine deaminase). The radioactivity of inosine, separated by thin-layer chromatography, is a measure of the adenosine initially present.
D000241:Adenosine / Q000032:analysis* / Q000097:blood; D000818:Animals; D002855:Chromatography, Thin Layer; D007124:Immunoenzyme Techniques; D066298:In Vitro Techniques; D009699:N-Glycosyl Hydrolases; D011683:Purine-Nucleoside Phosphorylase; D051381:Rats; D011919:Rats, Inbred Strains
C019603:adenosine phosphorylase; D011683:Purine-Nucleoside Phosphorylase; D009699:N-Glycosyl Hydrolases; C019599:adenosine nucleosidase; D000241:Adenosine