The effect of hypothalamic deafferentation on rat growth hormone-releasing factor (rGRF)-like immunoreactivity content in the medial basal hypothalamus of rats.
Rat growth hormone releasing factor (rGRF)- and somatostatin (SRIF)-like immunoreactivities (LI) were determined by radioimmunoassay in the medial basal hypothalamus (MBH) of the rat with either complete deafferentation (CD) or a sham operation. Two weeks after the surgery the mean amount of SRIF-LI in the isolated MBH was about 70% less than that in the sham-operated animals. On the other hand, the mean rGRF-LI in the MBH decreased only approximately 30% as compared to the levels in the sham-operated animals, the difference being statistically insignificant. These findings are consistent with anatomical evidence that the majority of the GRF perikarya are located in the arcuate nucleus, but a few are found outside the MBH, whereas the majority of the SRIF perikarya are located outside the MBH.
D000818:Animals; D003714:Denervation; D013007:Growth Hormone-Releasing Hormone / Q000032:analysis*; D007033:Hypothalamus, Middle / Q000032:analysis* / Q000502:physiology; D008297:Male; D009475:Neurons, Afferent / Q000502:physiology; D011863:Radioimmunoassay; D051381:Rats
D013007:Growth Hormone-Releasing Hormone