Protective mechanism of the immune response to a ribosomal vaccine from Pseudomonas aeruginosa. I. In vivo protection studies in compromised animal models.
Studies of the protective activity of a ribosomal vaccine from Pseudomonas aeruginosa in various immunocompromised animal models were performed. The results obtained demonstrated that the vaccine was highly effective in complement (C5)-deficient mice, C3-deficient (cobra venom factor-treated) mice, and leukopenic mice in providing protection against lethal infection with P. aeruginosa. Passive immunization with specific antiserum to the ribosomal vaccine was also effective in leukopenic mice. In mice that were both C5-deficient and leukopenic, the vaccine did not protect mice against lethal infection, but did prolong their survival, whereas in C3-deficient, leukopenic mice significantly enhanced protection was observed. These results were interpreted to suggest the involvement of multiple factors in the protective immune response to the vaccine, including the bactericidal and opsonic activities of specific antibody plus complement and the phagocytic activity of polymorphonuclear leukocytes with opsonized bacteria. Compensation for deficiencies in some of these factors can be obtained by enhancement of other factors through active or passive immunization.
D000818:Animals; D001428:Bacterial Vaccines / Q000276:immunology* / Q000302:isolation & purification; D003165:Complement System Proteins / Q000172:deficiency; D004195:Disease Models, Animal; D004353:Drug Evaluation, Preclinical; D004546:Elapid Venoms / Q000633:toxicity; D005260:Female; D007110:Immunity, Active / Q000187:drug effects; D007116:Immunization, Passive; D007153:Immunologic Deficiency Syndromes / Q000276:immunology*; D007970:Leukopenia / Q000276:immunology; D051379:Mice; D008811:Mice, Inbred DBA; D011552:Pseudomonas Infections / Q000517:prevention & control; D011550:Pseudomonas aeruginosa / Q000276:immunology*; D012270:Ribosomes / Q000276:immunology*
D001428:Bacterial Vaccines; D004546:Elapid Venoms; C016310:cobra venom factor; D003165:Complement System Proteins