Phosphorylation of actin-binding proteins by casein kinases 1 and 2.
Filamin and vinculin from chicken gizzards were significantly phosphorylated in vitro by casein kinases 1 and 2, but not by alpha-actinin. Antisera raised against these actin-binding proteins immunoprecipitated the phosphorylated proteins corresponding to filamin and vinculin, but no phosphoprotein corresponding to alpha-actinin was detected. These results suggest that filamin and vinculin are phosphorylated in vivo but alpha-actinin is not.
D000185:Actinin / Q000378:metabolism; D000199:Actins / Q000378:metabolism*; D000818:Animals; D047388:Casein Kinases; D002645:Chickens; D003285:Contractile Proteins / Q000378:metabolism; D064448:Filamins; D018260:Gelsolin; D005895:Gizzard, Avian; D007700:Kinetics; D008840:Microfilament Proteins / Q000378:metabolism*; D009124:Muscle Proteins / Q000378:metabolism; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism*; D016596:Vinculin
D000199:Actins; D003285:Contractile Proteins; D064448:Filamins; D018260:Gelsolin; D008840:Microfilament Proteins; D009124:Muscle Proteins; C016953:brevin; D000185:Actinin; D016596:Vinculin; D011494:Protein Kinases; D047388:Casein Kinases