title: Tamm Horsfall glycoprotein interferes with bacterial adherence to human kidney cells.
abstract: The effect of Tamm Horsfall protein (THP) of 18 healthy subjects and 14 diabetics on adherence of Escherichia coli (06:K13) 2699 strain to human kidney cells (HUK) was studied. Adhesion of bacteria (without additions: 100 bacteria per cell) was reduced dose-dependently by THP, half maximal inhibition occurring with 250 micrograms THP ml-1. Maximal inhibition (-84% at 1000 micrograms ml-1) exceeded inhibition by alpha-methyl-mannoside (36% at 50 mM), was specific (not reproduced by other glycoproteins, e.g. ovalbumin, mucin or thyroglobulin) and reversible (abolished by washing THP off HUK cells). Anti-adherence property of THP was not abolished by neuraminidase treatment. No significant difference of anti-adherence activity of THP was found between controls and diabetics, despite altered carbohydrate composition of THP in diabetes.
mesh_terms: D000328:Adult; D001422:Bacterial Adhesion / Q000187:drug effects*; D002478:Cells, Cultured; D003928:Diabetic Nephropathies / Q000188:drug therapy; D004926:Escherichia coli / Q000187:drug effects; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007668:Kidney / Q000166:cytology / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009088:Mucoproteins / Q000302:isolation & purification / Q000494:pharmacology*; D009439:Neuraminidase / Q000494:pharmacology; D058949:Uromodulin
chemical_list: D009088:Mucoproteins; C548612:UMOD protein, human; D058949:Uromodulin; D009439:Neuraminidase