Cell receptor specific targeted toxins: genetic construction and characterization of an interleukin 2 diphtheria toxin-related fusion protein.
We have genetically replaced the diphtheria toxin receptor binding domain with a DNA insert encoding the T-cell growth factor interleukin-2 (IL-2). The toxin-related IL-2 fusion gene encodes a 70,586 dalton protein, pro-IL-2-toxin. The mature form of IL-2-toxin is exported to the periplasmic space of recombinant Escherichia coli and has a molecular weight of 68,086. IL-2-toxin has been partially purified from periplasmic extracts of recombinant E. coli, and it is shown to contain immunologic determinants intrinsic to both its diphtheria toxin and IL-2 components. The chimeric toxin is targeted toward IL-2 receptor bearing T-cells in vitro.
D002460:Cell Line; D004274:DNA, Recombinant; D004167:Diphtheria Toxin / Q000235:genetics* / Q000494:pharmacology; D004926:Escherichia coli / Q000235:genetics; D005057:Eukaryotic Cells / Q000187:drug effects; D015852:Eukaryotic Initiation Factor-2; D005813:Genes, Synthetic; D007376:Interleukin-2 / Q000235:genetics*; D010448:Peptide Initiation Factors / Q000037:antagonists & inhibitors; D011506:Proteins / Q000037:antagonists & inhibitors; D011971:Receptors, Immunologic / Q000378:metabolism*; D015375:Receptors, Interleukin-2; D011993:Recombinant Fusion Proteins / Q000235:genetics* / Q000494:pharmacology; D011994:Recombinant Proteins / Q000235:genetics*
D004274:DNA, Recombinant; D004167:Diphtheria Toxin; D015852:Eukaryotic Initiation Factor-2; D007376:Interleukin-2; D010448:Peptide Initiation Factors; D011506:Proteins; D011971:Receptors, Immunologic; D015375:Receptors, Interleukin-2; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins