DNA repair synthesis in mouse spermatogenesis involves DNA polymerase beta activity.
The role of DNA polymerase alpha-DNA primase complex and DNA polymerase beta in DNA replication and ultraviolet-induced DNA repair synthesis has been analyzed in mouse spermatogenesis. Autoradiographic experiments with germ cells in culture, indicating an involvement of DNA polymerase alpha and/or delta in DNA replication, and of DNA polymerase beta in DNA repair synthesis, have been confirmed by studying partially purified enzymes. These findings support the idea that, different from other biological systems, in meiotic and post meiotic male mouse germ cells DNA polymerase beta is the main DNA polymerase form needed for DNA repair.
D000818:Animals; D016590:Aphidicolin; D001345:Autoradiography; D002454:Cell Differentiation; D002457:Cell Extracts; D002848:Chromatography, DEAE-Cellulose; D004256:DNA Polymerase I / Q000502:physiology*; D004257:DNA Polymerase II / Q000037:antagonists & inhibitors; D019915:DNA Primase; D004260:DNA Repair; D004261:DNA Replication; D004224:Diterpenes / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D005033:Ethylmaleimide / Q000494:pharmacology; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D012316:RNA Nucleotidyltransferases / Q000032:analysis; D013091:Spermatogenesis; D013942:Thymine Nucleotides / Q000494:pharmacology; D014466:Ultraviolet Rays
D002457:Cell Extracts; D004224:Diterpenes; D013942:Thymine Nucleotides; D016590:Aphidicolin; D019915:DNA Primase; D012316:RNA Nucleotidyltransferases; D004256:DNA Polymerase I; D004257:DNA Polymerase II; D005033:Ethylmaleimide; C024157:thymidine 5'-triphosphate