Murine B lymphoma cell lines release functionally active interleukin 2 after stimulation with Staphylococcus aureus.
In this study it is shown that an IL-2-like functional lymphokine activity derived from the murine B cell lines 2PK-3 and L10A2J after stimulation with Staphylococcus aureus can be blocked with anti-IL-2 mAb such as 9B11, DMS-1, and S4B6. Experiments demonstrate that the stimulation of the IL-2-sensitive cell line CTLL-2 by the IL-2-like functional activity derived from murine B cell tumors can also be blocked with the anti-IL-2R mAb PC61. Additionally, in RNA-RNA hybridization experiments with radiolabeled SP6-derived ssRNA probes developed from murine IL-2 genomic DNA sequences and specific for IL-2 mRNA, quantitatively significant amounts of IL-2-specific mRNA in both 2PK-3 and L10A2J are shown subsequent to stimulating the cells with S. aureus. These results suggest the murine B cell tumor lines 2PK-3 and L10A2J synthesize and release IL-2 after stimulation with selected polyclonal activators such as S. aureus.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D001402:B-Lymphocytes / Q000378:metabolism*; D001667:Binding, Competitive; D002460:Cell Line; D003602:Cytotoxicity, Immunologic; D007376:Interleukin-2 / Q000276:immunology / Q000378:metabolism* / Q000502:physiology; D008223:Lymphoma / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D012333:RNA, Messenger / Q000302:isolation & purification; D011971:Receptors, Immunologic / Q000502:physiology; D015375:Receptors, Interleukin-2; D013211:Staphylococcus aureus / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology
D000911:Antibodies, Monoclonal; D007376:Interleukin-2; D012333:RNA, Messenger; D011971:Receptors, Immunologic; D015375:Receptors, Interleukin-2