Lymphokine-induced MHC class II antigen expression on cultured retinal pigment epithelial cells and the influence of cyclosporin A.
Culture of rat or guinea-pig retinal pigment epithelium (RPE) cells with Con A-induced lymphokine derived from splenic lymphocytes or rat recombinant IFN-gamma induces strong MHC class II (Ia) antigen expression on up to 40% of RPE cells. Cyclosporin A, which inhibits intravitreal inflammation and Ia antigen expression on RPE cells in vivo, despite a choroidal T-cell infiltrate, suppresses production of Ia-inducing lymphokine but does not suppress in vitro responses of RPE cells to preformed lymphokine.
D000818:Animals; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D003524:Cyclosporins / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006168:Guinea Pigs; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D007371:Interferon-gamma / Q000494:pharmacology; D008222:Lymphokines / Q000494:pharmacology*; D010857:Pigment Epithelium of Eye / Q000187:drug effects / Q000276:immunology*; D051381:Rats; D011919:Rats, Inbred Strains
D003524:Cyclosporins; D000949:Histocompatibility Antigens Class II; D008222:Lymphokines; D003208:Concanavalin A; D007371:Interferon-gamma