The number of binding sites: the Klotz graph analysed.
A semilogarithmic plot (bound versus log of free ligand) has been proposed (Klotz, Science 217, 1247, 1982) to replace advantageously the Scatchard plot. We show that this representation has its own serious practical limitations and conclude that there are no "perfect" solutions: data have to be analysed in the light of all possible models using all available representations.
D001665:Binding Sites; D007700:Kinetics; D008024:Ligands; D008433:Mathematics; D008954:Models, Biological
D008024:Ligands