Sequence-specific cleavage of double helical DNA by triple helix formation.
Homopyrimidine oligodeoxyribonucleotides with EDTA-Fe attached at a single position bind the corresponding homopyrimidine-homopurine tracts within large double-stranded DNA by triple helix formation and cleave at that site. Oligonucleotides with EDTA.Fe at the 5' end cause a sequence specific double strand break. The location and asymmetry of the cleavage pattern reveal that the homopyrimidine-EDTA probes bind in the major groove parallel to the homopurine strand of Watson-Crick double helical DNA. The sequence-specific recognition of double helical DNA by homopyrimidine probes is sensitive to single base mismatches. Homopyrimidine probes equipped with DNA cleaving moieties could be useful tools for mapping chromosomes.
D001483:Base Sequence; D004247:DNA; D004492:Edetic Acid; D005296:Ferrous Compounds; D006801:Humans; D006868:Hydrolysis; D008875:Middle Aged; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides; D010957:Plasmids; D012997:Solvents
D005296:Ferrous Compounds; D009838:Oligodeoxyribonucleotides; D012997:Solvents; D004247:DNA; D004492:Edetic Acid