Detection of localization and internalization of membrane DNA in the Tetrahymena by the lactoferrin--colloidal gold technique.
It is known that lactoferrin binds to DNA. In the present study a lactoferrin-gold complex bound initially to the ciliary membrane, then appeared in coated pits and intracytoplasmic vesicles. On treatment for 1 h, the gold particles could be detected near to the nucleus. The lactoferrin-gold complex neither bound to the surface, nor appeared in the cytoplasm of those cells which had been exposed to DNAse for pretreatment.
D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D002923:Cilia / Q000378:metabolism; D003034:Coated Pits, Cell-Membrane / Q000378:metabolism; D004247:DNA / Q000378:metabolism*; D006046:Gold; D006651:Histocytochemistry; D007781:Lactoferrin; D008854:Microscopy, Electron; D013768:Tetrahymena / Q000378:metabolism* / Q000648:ultrastructure
D006046:Gold; D004247:DNA; D007781:Lactoferrin