title: A transgene containing lacZ inserted into the dystonia locus is expressed in neural tube.
abstract: The site of integration of transgenes in the host genome can affect levels of expression and occasionally confer ectopic patterns of expression on otherwise tissue-specific genes. We describe here a line of mice in which an hsp68-lacZ transgene is expressed in unstressed developing neural tissue and where the transgene insertion has caused a mutation of a neural tissue-specific gene, dystonia musculorum (dt). This coincidence suggests that expression of the hsp68-lacZ construct may be controlled directly by cis-acting regulatory sequences that normally control the developmental expression of the dt gene. Such constructs may serve as useful tools for identifying new tissue-specific enhancers and their associated genes.
mesh_terms: D000818:Animals; D002490:Central Nervous System / Q000196:embryology / Q000378:metabolism*; D003433:Crosses, Genetic; D004247:DNA; D004421:Dystonia / Q000235:genetics*; D007763:Lac Operon; D051379:Mice; D008822:Mice, Transgenic; D009154:Mutation; D012045:Regulatory Sequences, Nucleic Acid; D001616:beta-Galactosidase / Q000096:biosynthesis / Q000235:genetics
chemical_list: D004247:DNA; D001616:beta-Galactosidase