title: Characteristics of H+ current transients induced by adverse H+ gradient pulses in toad bladder.
abstract: Acidification in the toad bladder occurs as a result of electrogenic H+ secretion (JH). When a pH gradient is applied in a stepwise fashion in the absence of exogenous CO2, JH decreases linearly with the mucosal (M) solution pH and is null when pHm is approximately 4.5. When pHm is returned to initial values (7.4) in a stepwise fashion, JH increases linearly with pHm. However, on this return, higher values of JH are initially obtained. To investigate this hysteresis, hemibladders mounted in chambers were used to measure the change in the H+ current before and after acid pulses were applied to the mucosal solution. In the absence of exogenous CO2, the application of graded acid pulses to mucosa for 1, 2, 4, and 8 min resulted in a graded decrease in JH. The restoration of pHm to 7.4 was followed by an immediate transient overshoot of reversed short-circuit current (Irsc), which was related to the time of exposure and the magnitude of the acid pulse. The longer the acid pulse or the larger the pulse, the greater the Irsc overshoot. The addition of protonophores, dinitrophenol, or salicylate, into the mucosal solution enhanced this overshoot. Similar Irsc overshoots could be obtained with the application of pulses of adverse electrical gradients. Introduction of exogenous CO2 into the system (3%) completely inhibited the overshoot in JH after an acid pulse. In conclusion, when pHm is decreased JH is reduced and the cell pH presumably decreases because of continued exit of alkali at the serosal side of the cell and entry of H+ from the mucosal solution. The decrease in cell pH then triggers the pump to produce a sharp overshoot in JH when pHm returns to 7.4.
mesh_terms: D019297:2,4-Dinitrophenol; D000818:Animals; D001639:Bicarbonates / Q000378:metabolism; D002024:Bufo marinus; D004140:Dinitrophenols / Q000494:pharmacology; D004563:Electrochemistry; D006859:Hydrogen / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008433:Mathematics; D012459:Salicylates / Q000494:pharmacology; D020156:Salicylic Acid; D001743:Urinary Bladder / Q000187:drug effects / Q000378:metabolism*
chemical_list: D001639:Bicarbonates; D004140:Dinitrophenols; D012459:Salicylates; D006859:Hydrogen; D020156:Salicylic Acid; D019297:2,4-Dinitrophenol