Conjugal transfer of group B streptococcal plasmids and comobilization of Escherichia coli-Streptococcus shuttle plasmids to Lactobacillus plantarum.
The antibiotic resistance group B streptococcal plasmids, pIP501 and pVA797, were conjugally transferred from Streptococcus faecalis to Lactobacillus plantarum. The Escherichia coli-Streptococcus shuttle plasmids, pVA838 and pSA3, were mobilized from S. sanguis to L. plantarum by pVA797 via cointegrate formation. pVA838 readily resolved from pVA797 and was present in L. plantarum as deletion derivatives. The pVA797::pSA3 cointegrate failed to resolve in L. plantarum.
D003227:Conjugation, Genetic; D004269:DNA, Bacterial / Q000032:analysis; D004587:Electrophoresis, Agar Gel; D013293:Enterococcus faecalis / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors; D007778:Lactobacillus / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D011815:R Factors; D013291:Streptococcus / Q000235:genetics*; D013292:Streptococcus agalactiae / Q000235:genetics; D013298:Streptococcus sanguis / Q000235:genetics; D014162:Transfection
D004269:DNA, Bacterial