Schistosoma mansoni: human serum and release of proteins from schistosomula.
Radioiodinated surface proteins of schistosomula of Schistosoma mansoni, including those involved in low density lipoprotein binding, are released into a defined culture medium at 37 C at slower rates than proteins from schistosomula incubated in a serum-containing medium. When schistosomula were incubated at 4 C, a higher degree of reassociation of the same proteins into the parasites was detected. Purified human low density lipoproteins and a fraction containing very low density lipoprotein plus chylomicrons were also able to promote the release of iodinated proteins from the parasites. These observations suggest that serum may play a role in accelerating the turnover of membrane components in schistosomula.
D000818:Animals; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008077:Lipoproteins, LDL / Q000378:metabolism; D008079:Lipoproteins, VLDL / Q000378:metabolism; D008565:Membrane Proteins / Q000302:isolation & purification / Q000378:metabolism; D012550:Schistosoma mansoni / Q000502:physiology*; D012555:Schistosomiasis mansoni / Q000097:blood / Q000469:parasitology
D003470:Culture Media; D008077:Lipoproteins, LDL; D008079:Lipoproteins, VLDL; D008565:Membrane Proteins