Isolation and characterization of "Methanosphaera cuniculi" sp. nov.
A nonmotile, gram-positive, spherical organism was isolated from the intestinal tracts of rabbits. Both hydrogen and methanol were required for growth. No methane was produced from hydrogen-carbon dioxide, formate, acetate, methylamines, ethanol, or isopropanol. The optimum pH was 6.8, and the optimum temperature was 35 to 40 degrees C. The DNA G+C content is 23 mol%. The pseudomurein cell wall contained serine. These characteristics and the immunological fingerprinting results are consistent with its placement in the genus Methanosphaera as a new species.
D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis; D002473:Cell Wall / Q000032:analysis; D004269:DNA, Bacterial / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D019605:Euryarchaeota / Q000145:classification / Q000254:growth & development / Q000302:isolation & purification* / Q000648:ultrastructure; D006859:Hydrogen / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007422:Intestines / Q000382:microbiology*; D008697:Methane / Q000096:biosynthesis; D000432:Methanol / Q000378:metabolism; D008854:Microscopy, Electron; D010084:Oxidation-Reduction; D011817:Rabbits / Q000382:microbiology*; D012694:Serine / Q000032:analysis; D013696:Temperature
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D012694:Serine; D006859:Hydrogen; D008697:Methane; D000432:Methanol