Receptors for lactogenic hormones in the ovine corpus luteum. I: A major discrepancy in the specific binding of radiolabelled ovine prolactin and human growth hormone.
The characteristics of the binding of 125I-labelled human GH (hGH) and ovine prolactin (oPRL) were studied in the ovine corpus luteum. Although oPRL is the homologous ligand for sheep lactogenic receptors, its binding was significantly and consistently lower than that of 125I-labelled hGH. This was not due to iodination damage of oPRL since: (1) 125I-labelled oPRL tracers which bound poorly relative to 125I-labelled hGH in the ovine corpus luteum were equipotent in the pig and rat corpus luteum, (2) the differences between 125I-labelled hGH and oPRL binding persisted with tracers of equivalent biopotency and (3) the iodination procedure affected neither oPRL bioactivity in the Nb2 tumour assay nor its binding activity with ovine corpus luteum receptors. Ovine luteal receptors were specific for lactogenic hormones. The specific binding of 125I-labelled hGH or oPRL could be inhibited completely by incubation with either unlabelled hormone, with similar potencies. However, oGH inhibited binding only at much higher concentrations, consistent with its known contamination with oPRL. Moreover, 125I-labelled oGH was not bound specifically to sheep luteal tissue. Fractionation of sheep luteal homogenates on sucrose density gradients (with or without cell-surface membrane perturbation by digitonin) demonstrated that binding of 125I-labelled hGH and 125I-labelled oPRL peaked in the same regions of the gradients, coincident with a number of luteal cell-surface membrane markers. We conclude that the marked discrepancy between the binding of hGH and oPRL tracers by sheep luteal tissue was not due to iodination damage of oPRL, binding of 125I-labelled hGH to somatogenic receptors or differential binding to luteal cell-surface versus intracellular receptors.
D000818:Animals; D002413:Cations, Divalent / Q000494:pharmacology; D002462:Cell Membrane / Q000378:metabolism; D003338:Corpus Luteum / Q000378:metabolism*; D004492:Edetic Acid / Q000494:pharmacology; D005260:Female; D013006:Growth Hormone / Q000378:metabolism*; D007457:Iodine Radioisotopes; D011388:Prolactin / Q000378:metabolism*; D011981:Receptors, Prolactin / Q000378:metabolism*; D011986:Receptors, Somatotropin / Q000378:metabolism; D012756:Sheep
D002413:Cations, Divalent; D007457:Iodine Radioisotopes; D011981:Receptors, Prolactin; D011986:Receptors, Somatotropin; D011388:Prolactin; D013006:Growth Hormone; D004492:Edetic Acid