[Determination of antibodies to mycobacterial antigens in tuberculosis by erythrocyte immunoadsorption with a rosette marker].
A solid-phase enzyme immunoassay system for the determination of antibodies to mycobacterial antigens, based on the method of erythrocyte immunoadsorption in microchambers for immunological reactions, has been developed. To detect antibodies specifically bound with the solid-phase antigen, the affinity rosettes of Staphylococcus aureus strain Cowan I, carrying protein A, with erythrocytes conjugated with human gamma globulin have been used. The significant correlation of the titers of 34 sera, determined by means of erythrocyte immunoadsorption, with extinction values obtained in the solid-phase enzyme immunoassay of antibodies to Mycobacterium tuberculosis has been established. The coincidence of the results in 92% of cases has been noted.
D000907:Antibodies, Bacterial / Q000032:analysis*; D000942:Antigens, Bacterial / Q000276:immunology*; D015415:Biomarkers / Q000032:analysis; D003937:Diagnosis, Differential; D004912:Erythrocytes / Q000276:immunology*; D005069:Evaluation Studies as Topic; D006801:Humans; D007124:Immunoenzyme Techniques; D007163:Immunosorbent Techniques / Q000295:instrumentation; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D012397:Rosette Formation; D013211:Staphylococcus aureus / Q000276:immunology; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis*; D014401:Tuberculosis, Urogenital / Q000175:diagnosis*
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D015415:Biomarkers
Opredelenie antitel k antigenam mikobakteriĭ pri tuberkuleze metodom éritroimmunoadsorbtsii s rozetochnym markerom.