title: Deletion analysis of the achaete-scute locus of Drosophila melanogaster.
abstract: The achaete-scute gene complex (AS-C) is involved in the development of the central and peripheral (sensory chaetae, sensilla) nervous system. To assess the contribution of the different parts of the complex in the generation of the adult chaetae pattern, we have determined the phenotypes and molecular positions of the breakpoints of 74 terminal deficiencies of the X chromosome. According to these and previous data, the AS-C is organized, distally to proximally, as follows: the achaete region, with most of its DNA (10 kb) located upstream from the putative achaete (T5) gene; an intermediate region, approximately 18 kb long, whose deletion only weakly affects the scute function; and the scute region, with most of the DNA critical for its function extending 4-5 kb upstream and 50 kb downstream of the putative scute (T4) gene. The DNA extending far upstream of the T5 gene and downstream of the T4 gene may provide chromatin conformations adequate for efficient expression of these genes. However, in the case of the T4 gene, the available data suggest the presence of a small number of elements, scattered in the long downstream region, that would respond to topological cues and cis-activate this gene in specific anatomical regions.
mesh_terms: D000818:Animals; D002872:Chromosome Deletion; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004252:DNA Mutational Analysis; D004331:Drosophila melanogaster / Q000196:embryology / Q000235:genetics* / Q000254:growth & development; D009024:Morphogenesis; D009420:Nervous System / Q000196:embryology; D010641:Phenotype; D012333:RNA, Messenger / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid; D014960:X Chromosome
chemical_list: D012333:RNA, Messenger