[Lyophilization of dermatophytes].
When 16 dermatophyte strains were lyophilized, the highest survival was recorded in cultures where microconidia constituted a prevalent part. The viability was considerably reduced by lyophilization in the strains producing mainly macroconidia, chlamydospores and hyphae. A 2.5% solution of gelatine + 3.75% solution of saccharose proved to be an effective protecting lyophilization medium for the cultures of Trichophyton mentagrophytes and T. verrucosum to be lyophilized and maintained for two years. Very good results were also obtained when saccharose solutions (3.75, 7.5 and 15%) were used alone; however, an increased relative residual moisture content of the lyophilizates was recorded in some samples. Lyophilized cultures of dermatophytes were stored with success at refrigerator temperatures. When the lyophilizates were kept at laboratory temperature, the viability of the strains was reduced substantially irrespective of their being kept in the dark or exposed to light. The nutrient medium for the reconstitution of the lyophilized strains should be chosen on the basis of the growth requirements of individual cultures. In our trials the best results were obtained with the sweet wort media and the Sabouraud agar; the best media imported from foreign countries were the Mycological agar and Mycobiotic agar.
D005612:Freeze Drying; D014249:Trichophyton / Q000502:physiology*
Lyofilizace dermatofytú.