Purification of anthrax-toxin components by high-performance anion-exchange, gel-filtration and hydrophobic-interaction chromatography.
A procedure has been developed for purification of the tripartite anthrax-toxin components. This involves sequential high-performance anion-exchange, gel-filtration and hydrophobic-interaction chromatography. From an initial culture volume of 15 litres, typical yields of 8 mg of protective antigen, 13 mg of lethal factor and 7 mg of oedema factor are produced to higher degrees of purity than have previously been achieved by conventional chromatographic techniques.
D000818:Animals; D000881:Anthrax / Q000378:metabolism*; D000942:Antigens, Bacterial; D001427:Bacterial Toxins / Q000302:isolation & purification* / Q000633:toxicity; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D005779:Immunodiffusion; D051379:Mice
D000942:Antigens, Bacterial; D001427:Bacterial Toxins; C030325:anthrax toxin