Chondroitin sulphate at the endothelial lumen of pig aorta: assay by radiolabelling and by X-ray microanalysis.
Trypsin-releasable glycosaminoglycans from the luminal surface of intact pig aorta were measured following metabolic labelling with [35S]sulphate. Chondroitin sulphate was found to be present at a surface density equal to that already established for heparan sulphate (5 X 10(11) chains per cm2). This result was confirmed by X-ray microanalysis of the luminal sulphur content before and after treatment with specific glycosaminoglycan-degrading enzymes. This result implies that approximately half of the luminal surface is occupied by sulphated glycosaminoglycans.
D000818:Animals; D001013:Aorta, Thoracic / Q000032:analysis; D002807:Chondroitin / Q000031:analogs & derivatives*; D002809:Chondroitin Sulfates / Q000032:analysis*; D004577:Electron Probe Microanalysis; D004730:Endothelium, Vascular / Q000032:analysis*; D005260:Female; D008297:Male; D010206:Papain; D013431:Sulfates / Q000032:analysis; D013462:Sulfur Radioisotopes / Q000032:analysis; D013552:Swine; D014357:Trypsin
D013431:Sulfates; D013462:Sulfur Radioisotopes; D002807:Chondroitin; D002809:Chondroitin Sulfates; D014357:Trypsin; D010206:Papain