Crosslinking of concanavalin A with glutaraldehyde.
Crosslinking of Concanavalin A with low concentrations of glutaraldehyde gives a mixture of products. A specific product having about 66% of the biological activity of the native molecule was characterized. Sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis showed the presence of monomers, dimers, trimers, tetramers, and a small amount of pentamers as products. The presence of alpha-methyl mannoside during crosslinking changed the nature of the products, yielding a product retaining 80% of the biological activity. The crosslinked products showed greater stability than the native molecule at alkaline pH. However, the greatest stability under alkaline conditions was shown by the native molecule itself where alpha-methyl mannoside was present.
D000447:Aldehydes; D003208:Concanavalin A / Q000302:isolation & purification; D003432:Cross-Linking Reagents; D005976:Glutaral; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D008766:Methylmannosides; D011487:Protein Conformation; D011489:Protein Denaturation
D000447:Aldehydes; D003432:Cross-Linking Reagents; D008766:Methylmannosides; D003208:Concanavalin A; C008466:methylmannoside; D005976:Glutaral