Serum capacity to solubilize immune complexes (ICSC) in patients with insulin-dependent diabetes mellitus.
Immune complex solubilizing capacity (ICSC) of sera obtained from patients with insulin-dependent diabetes mellitus (IDDM) was assayed by the spectrophotometric method. In 17/32 IDDM sera, ICSC was significantly low. The value of ICSC was only weakly correlated with serum CH50 (r = 0.42). Six sera out of 28 showed a high serum immune complex level and low ICSC. The results with these 6 cases suggest the possibility that circulating antigen-antibody complex might be insoluble in vivo. The precise characterization of these 6 patients' autoantibodies was obtained with immunofluorescence tests. Interestingly, 2 of these sera were positive for islet cell surface antibodies (ICSA) and AtT-20 cell (ACTH secreting cell) surface antibodies.
D000906:Antibodies / Q000032:analysis; D000936:Antigen-Antibody Complex; D002460:Cell Line; D003922:Diabetes Mellitus, Type 1 / Q000097:blood / Q000276:immunology*; D006801:Humans; D007106:Immune Sera; D007515:Islets of Langerhans / Q000276:immunology; D012016:Reference Values; D012995:Solubility; D013053:Spectrophotometry; D001616:beta-Galactosidase / Q000276:immunology
D000906:Antibodies; D000936:Antigen-Antibody Complex; D007106:Immune Sera; D001616:beta-Galactosidase