Motility of leukemic cells in collagen gel related to immunological phenotype in childhood acute lymphoblastic leukemia.
Motility of leukemic cells was measured in a three-dimensional collagen matrix assay. Leukemic cells from 16 children with acute lymphoblastic leukemia (ALL) and normal peripheral blood lymphocytes (NPBL) from 6 healthy volunteers, were allowed to migrate into this collagen matrix for 48 h at 37 degrees C. NPBL migrated much further (300-600 micron) than leukemic cells (0-200 micron). Among the leukemic cases, only common ALL and one case of null ALL showed some migration (0-200 micron). T-All cells did not migrate at all under the circumstances of this experiment.
D000951:Antigens, Neoplasm / Q000032:analysis; D000954:Antigens, Surface / Q000032:analysis; D002465:Cell Movement; D002648:Child; D003094:Collagen; D005782:Gels; D006801:Humans; D007945:Leukemia, Lymphoid / Q000145:classification / Q000276:immunology / Q000473:pathology*; D008214:Lymphocytes / Q000276:immunology / Q000473:pathology*; D010641:Phenotype
D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D005782:Gels; D003094:Collagen