New plasmid vectors for the construction of translational gene fusions in Bacillus subtilis.
A series of plasmids has been constructed that can be used to fuse the lacZ gene of Escherichia coli to Bacillus subtilis genes, in all three transcriptional reading frames. Gene expression can be monitored, after gene fusion, by measuring beta-galactosidase activity.
D001412:Bacillus subtilis / Q000235:genetics*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005786:Gene Expression Regulation; D005822:Genetic Vectors; D007763:Lac Operon; D010957:Plasmids; D014176:Protein Biosynthesis; D001616:beta-Galactosidase / Q000235:genetics
D001616:beta-Galactosidase