Monoclonal antibody to an aminoglycoside-resistance factor from Pseudomonas aeruginosa.
Aminoglycoside resistance (AMGr) in Pseudomonas aeruginosa is most commonly due to a membrane permeability factor. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of outer membrane proteins (OMPs) from AMGr strains of P. aeruginosa (resistant to tobramycin, gentamicin, and amikacin) revealed a unique OMP of 42 kilodaltons (kDa) when compared with aminoglycoside-susceptible strains of P. aeruginosa. A monoclonal antibody (MAb) to an AMGr strain of P. aeruginosa was prepared and used to study AMGr. This MAb, PS1, recognized a unique OMP of 42 kDa present primarily in AMGr strains. MAb PS1 had a binding sensitivity of 83% (n = 46), for AMGr P. aeruginosa, and a specificity of 85% (n = 36), as determined by immunoblot assay. This MAb may be useful for investigating the basis of AMGr in P. aeruginosa.
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000907:Antibodies, Bacterial / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D002463:Cell Membrane Permeability; D004352:Drug Resistance, Microbial / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D015151:Immunoblotting; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000235:genetics / Q000276:immunology*; D011815:R Factors
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D001425:Bacterial Outer Membrane Proteins