Induction of the heat-shock response by carbon dioxide in Chironomus thummi.
The effects of a set of stress treatments on gene expression of Chironomus thummi salivary gland cells have been analyzed. Among the treatments assayed, only during recovery from carbon dioxide have we observed a response similar to that previously described after heat-shock treatment: induction of the heat-shock puffs and synthesis of the heat-shock polypeptides. In these conditions, puffing and transcription of telomeric regions were observed, which led to the appearance of the temperature-inducible telomeric Balbiani ring T-BR-III. Other treatments failed to induce the heat-shock response, despite promoting real stress conditions to C. thummi larvae or salivary gland cells.
D000818:Animals; D002245:Carbon Dioxide / Q000494:pharmacology*; D002683:Chironomidae / Q000187:drug effects* / Q000235:genetics; D002875:Chromosomes / Q000187:drug effects / Q000648:ultrastructure*; D004247:DNA / Q000235:genetics*; D004175:Diptera / Q000187:drug effects*; D004591:Electrophoresis, Polyacrylamide Gel; D006360:Heat-Shock Proteins / Q000096:biosynthesis* / Q000235:genetics; D009693:Nucleic Acid Hybridization; D013462:Sulfur Radioisotopes
D006360:Heat-Shock Proteins; D013462:Sulfur Radioisotopes; D002245:Carbon Dioxide; D004247:DNA