Factors regulating the secretion of lysophosphatidylcholine by rat hepatocytes compared with the synthesis and secretion of phosphatidylcholine and triacylglycerol. Effects of albumin, cycloheximide, verapamil, EGTA and chlorpromazine.
1. The synthesis and secretion of glycerolipid by monolayer cultures of rat hepatocytes was measured by determining the incorporations of [3H]glycerol, [3H]oleate and [14C]choline and by the absolute concentration of triacylglycerol. 2. The presence of albumin in the medium stimulated the accumulation of lysophosphatidylcholine in the medium by 11-13-fold. 3. Cycloheximide did not significantly alter the accumulation of lysophosphatidylcholine. 4. This process was particularly sensitive to inhibition by chlorpromazine and verapamil, compared with the secretion of triacylglycerol and phosphatidylcholine. By contrast, it was relatively less sensitive to EGTA. 5. It is suggested that intracellular Ca2+ may be important in the production of lysophosphatidylcholine, which then accumulates in the medium by binding to albumin. In vivo this lysophosphatidycholine may be a means of delivering choline and polyunsaturated fatty acids to other organs.
D000418:Albumins / Q000494:pharmacology; D000818:Animals; D002478:Cells, Cultured; D002746:Chlorpromazine / Q000494:pharmacology; D002794:Choline / Q000378:metabolism; D003513:Cycloheximide / Q000494:pharmacology; D004533:Egtazic Acid / Q000494:pharmacology; D005990:Glycerol / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008244:Lysophosphatidylcholines / Q000378:metabolism*; D019301:Oleic Acid; D009829:Oleic Acids / Q000378:metabolism; D010713:Phosphatidylcholines / Q000378:metabolism*; D051381:Rats; D014280:Triglycerides / Q000378:metabolism*; D014700:Verapamil / Q000494:pharmacology
D000418:Albumins; D008244:Lysophosphatidylcholines; D009829:Oleic Acids; D010713:Phosphatidylcholines; D014280:Triglycerides; D019301:Oleic Acid; D004533:Egtazic Acid; D003513:Cycloheximide; D014700:Verapamil; D002794:Choline; D005990:Glycerol; D002746:Chlorpromazine