[Stepwise synthesis of oligonucleotides. XXXIV. Preparative synthesis of trinucleoside diphosphates and longer oligoribonucleotides using immobilized ribonucleases].
Immobilized guanyl-specific ribonucleases from Aspergillus clavatus (C2), A. oryzae (T1), and Bacillus intermedius 7P (Bi) have been used for preparative synthesis of ten trinucleoside diphosphates, three tetra- and one pentanucleotide having the only guanylic acid residue at the 5'-end. The nucleotide sequence of the oligonucleotide synthesised determined the choice of the ribonuclease.
D055598:Chemical Phenomena; D002621:Chemistry; D004722:Endoribonucleases; D004800:Enzymes, Immobilized; D009843:Oligoribonucleotides / Q000138:chemical synthesis*; D006163:Ribonuclease T1
D004800:Enzymes, Immobilized; D009843:Oligoribonucleotides; D004722:Endoribonucleases; D006163:Ribonuclease T1
Stupenchatyĭ sintez oligonukleotidov. XXXIV. Preparativnyĭ sintez trinukleoziddifosfatov i bolee dlinnykh oligoribonukleotidov s immobilizovannymi ribonukleazami.