The 5-hydroxytryptamine stimulated formation of inositol phosphate is inhibited in platelets from alcoholics.
The accumulation of inositol monophosphate (IP1) was measured after stimulation of 5-hydroxytryptamine2 (5-HT2) receptors on platelets from alcoholics and healthy controls. In controls, 5-HT induced a dose-dependent response with an EC50 = 2 x 10(-6) M and a maximal response at 10(-5) M. Ritanserin, a selective 5-HT2 antagonist, markedly reduced the accumulation. The IP1 formation after stimulation by 10(-5) M 5-HT was significantly impaired in platelets from alcoholics as compared to controls. This study indicates that the 5-HT2 receptor function is inhibited in alcoholics. It also illustrates the possibility of using IP1 formation in peripheral cells as a mean of studying receptor function in disease.
D000328:Adult; D000437:Alcoholism / Q000097:blood*; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D007295:Inositol Phosphates / Q000097:blood*; D008297:Male; D008875:Middle Aged; D010880:Piperidines / Q000494:pharmacology; D011985:Receptors, Serotonin / Q000187:drug effects / Q000502:physiology; D016713:Ritanserin; D012701:Serotonin / Q000494:pharmacology*; D013403:Sugar Phosphates / Q000097:blood*
D007295:Inositol Phosphates; D010880:Piperidines; D011985:Receptors, Serotonin; D013403:Sugar Phosphates; D016713:Ritanserin; D012701:Serotonin