Influence of long term treatment with 3-methylcholanthrene or carbaryl on mixed-function oxidase activity in 3T3 fibroblasts: studies by microspectrofluorimetry on single cells.
Using benzo(a)pyrene (BaP) as a probe for aryl hydrocarbon hydroxylase (AHH) activity, differences in mixed-function oxidase (MFO) activity were observed using microspectrofluorimetry in single living cells during long term treatment with 3-methylcholanthrene (3-MC) or carbaryl. Although these two compounds differ in chemical structure, similar effects were observed in 3T3 cell populations. The results suggest that the two compounds activate the same enzymatic system and that individual cells of a supposed homogeneous cell population are not equally sensitive to xenobiotics, i.e. subpopulations were observed which have differences in AHH activity.
D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases / Q000032:analysis* / Q000037:antagonists & inhibitors; D001564:Benzo(a)pyrene; D012721:Carbaryl / Q000633:toxicity*; D002478:Cells, Cultured; D005347:Fibroblasts / Q000187:drug effects; D007700:Kinetics; D008748:Methylcholanthrene / Q000633:toxicity*; D051379:Mice; D006899:Mixed Function Oxygenases / Q000032:analysis* / Q000037:antagonists & inhibitors; D013050:Spectrometry, Fluorescence; D013997:Time Factors
D001564:Benzo(a)pyrene; D008748:Methylcholanthrene; D006899:Mixed Function Oxygenases; D001189:Aryl Hydrocarbon Hydroxylases; D012721:Carbaryl