Effects of phorbol esters on B-cell gene expression.
Splenic B lymphocytes were stimulated with lipopolysaccharide alone or in combination with phorbol-12,13-dibutyrate, a protein kinase C-activating phorbol ester. The effect of the treatment was analysed at the single cell level with in situ RNA/RNA hybridization. Hybridization with a kappa light chain probe revealed that the whole population had shifted towards a low, but significant, expression of immunoglobulin mRNA. Analysis at the population level was performed by DNA/RNA and RNA/RNA hybridization experiments. It was found that the steady-state levels of mRNA for kappa light chain, IgM heavy chain and J chain were reduced by phorbol ester treatment, while the steady-state level of mRNA for IgD heavy chain was increased. Steady-state levels of mRNA for Ia antigen and alpha-actin were marginally affected.
D000199:Actins / Q000235:genetics; D000818:Animals; D001402:B-Lymphocytes / Q000502:physiology*; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D005786:Gene Expression Regulation / Q000187:drug effects; D005803:Genes, Immunoglobulin; D000949:Histocompatibility Antigens Class II / Q000235:genetics; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D007147:Immunoglobulin Light Chains / Q000235:genetics; D066298:In Vitro Techniques; D051379:Mice; D009693:Nucleic Acid Hybridization; D015240:Phorbol 12,13-Dibutyrate / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics; D013154:Spleen / Q000166:cytology
D000199:Actins; D000949:Histocompatibility Antigens Class II; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D012333:RNA, Messenger; D015240:Phorbol 12,13-Dibutyrate