Nitrilotriacetic acid (NTA) does not induce chromosomal damage in mammalian cells either in vitro or in vivo.
We used human lymphocyte cultures to repeat the experiments under the very particular conditions of nitrilotriacetic acid (NTA) treatments (high doses: up to 10(-2) M; very long exposure times: up to 5 days) which have been described as being able to induce chromosomal aberrations, and we also performed the more conventional treatments (24-48 h of exposure) as suggested in the protocols adopted by the EEC-OECD. Mitomycin C was routinely used as a positive clastogenic control. NTA did not significantly increase the frequency of chromosomal aberrations in any of the different experimental conditions adopted. Furthermore, no induction of micronuclei was observed in mouse polychromatic erythrocytes after treatment in vivo for up to 48 h with NTA (200-400 mg/kg b.w.), whereas the frequency of micronuclei was significantly increased by mitomycin C (1 mg/kg b.w.).
D000085:Acetates / Q000494:pharmacology*; D000328:Adult; D000818:Animals; D002467:Cell Nucleus / Q000187:drug effects; D002869:Chromosome Aberrations; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects*; D008297:Male; D051379:Mice; D016685:Mitomycin; D008937:Mitomycins / Q000494:pharmacology; D009571:Nitrilotriacetic Acid / Q000494:pharmacology*
D000085:Acetates; D008937:Mitomycins; D016685:Mitomycin; D009571:Nitrilotriacetic Acid