Mechanism of thrombin-induced rise in platelet fructose 2,6-bisphosphate content. Studies using phorbol myristate acetate, dioctanoylglycerol and ionophore A23187.
The mechanism by which thrombin increases platelet fructose 2,6-bisphosphate content was investigated. The action of thrombin was mimicked by phorbol 12 myristate 13-acetate and 1,2-dioctanoylglycerol. Ca2+ with A23187 potentiated the action of both these compounds. The action of thrombin required mobilization of intracellular and extracellular Ca2+ and was not decreased by indomethacin. This study suggests that protein kinase C activation and Ca2+ mobilization are both involved in the activation of glycolysis by thrombin.
D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism*; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000494:pharmacology; D004075:Diglycerides / Q000494:pharmacology; D004533:Egtazic Acid / Q000494:pharmacology; D005635:Fructosediphosphates / Q000097:blood*; D006019:Glycolysis / Q000187:drug effects; D006598:Hexosediphosphates / Q000097:blood*; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013917:Thrombin / Q000494:pharmacology*
D004075:Diglycerides; D005635:Fructosediphosphates; D006598:Hexosediphosphates; C044295:1,2-dioctanoylglycerol; D000001:Calcimycin; D004533:Egtazic Acid; C027652:fructose 2,6-diphosphate; D013917:Thrombin; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium; D007213:Indomethacin