Puromycin enhances 20-hydroxyecdysone-induced protein synthesis in wing discs of Drosophila melanogaster.
The effect of cycloheximide and puromycin on 20-hydroxyecdysone-induced protein synthesis in wing discs of Drosophila melanogaster has been studied by one-dimensional and two-dimensional SDS polyacrylamide electrophoresis. It is found that puromycin, but not cycloheximide, when applied simultaneously with the hormone enhanced the hormone-induced synthesis of the 'early' and 'late' proteins. However, when puromycin was applied after hormone treatment, only the 'late' proteins were induced. The possible implication of these observations is discussed.
D000818:Animals; D003513:Cycloheximide / Q000494:pharmacology; D004331:Drosophila melanogaster / Q000254:growth & development / Q000378:metabolism*; D004357:Drug Synergism; D004441:Ecdysterone / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects*; D007814:Larva; D009924:Organ Culture Techniques; D014176:Protein Biosynthesis; D011691:Puromycin / Q000494:pharmacology*; D014158:Transcription, Genetic / Q000187:drug effects; D014921:Wings, Animal
D011691:Puromycin; D004441:Ecdysterone; D003513:Cycloheximide