Influence of beta-D-xyloside on growth and histological aspect of long bones in chicken embryos.
It is known that beta-D-xylosides interfere with the proteoglycan synthesis in several tissues. A possible influence of this disturbed synthesis on the matrix formation of bone and cartilage has not been described light microscopically. In the present study we used 10-day-old chicken embryos which were exposed in ovo to a final concentration of 0.5 mM beta-D-xyloside. After 3, 6, 9, 20, 25, 31, 35 and 40 days, lengths of several skeletal elements were determined and the middle metatarsal bones were processed for light microscopical demonstration of acidic groups. The results demonstrate that beta-D-xyloside inhibits growth of long bones and induces synthesis of a cartilage matrix with a very low concentration of chondroitin sulphate. It has no noticeable influence on the amount of acidic groups in the organic bone matrix. Despite the absence of chondroitin sulphate, the cartilage matrix becomes mineralized normally.
D000818:Animals; D001846:Bone Development / Q000187:drug effects*; D001842:Bone and Bones / Q000002:abnormalities* / Q000187:drug effects / Q000378:metabolism; D002113:Calcification, Physiologic / Q000187:drug effects; D002356:Cartilage / Q000187:drug effects / Q000378:metabolism; D002642:Chick Embryo; D002809:Chondroitin Sulfates / Q000378:metabolism; D006027:Glycosides / Q000633:toxicity*; D011509:Proteoglycans / Q000378:metabolism; D013723:Teratogens
D006027:Glycosides; D011509:Proteoglycans; D013723:Teratogens; C008646:4-nitrophenyl beta-D-xyloside; D002809:Chondroitin Sulfates