Thialysine and selenalysine incorporation into CHO cell proteins and its effects on cell growth.
CHO cells can incorporate into proteins both thialysine and selenalysine when both are present together in the culture medium. Thialysine and selenalysine inhibit cell growth and cell viability. The inhibitory effect of either analog is additive. The inhibition of cell viability is related to the extent of protein lysine substitution by thialysine or selenalysine; it is however irrelevant whether lysine is substituted by one or the other analog or by both.
D000818:Animals; D002455:Cell Division; D002460:Cell Line; D002470:Cell Survival; D006224:Cricetinae; D003545:Cysteine / Q000031:analogs & derivatives* / Q000378:metabolism; D006131:Growth Inhibitors; D008239:Lysine / Q000031:analogs & derivatives* / Q000378:metabolism; D016566:Organoselenium Compounds; D014176:Protein Biosynthesis; D012643:Selenium / Q000378:metabolism*
D006131:Growth Inhibitors; D016566:Organoselenium Compounds; C000204:S-2-aminoethyl cysteine; C011351:selenalysine; D012643:Selenium; D008239:Lysine; D003545:Cysteine