title: AL preincubation inhibits calcification of bioprosthetic heart valve tissue in the rat subdermal model.
abstract: To date, the clinical usefulness of bioprosthetic heart valves (BPV) fabricated from glutaraldehyde pretreated porcine aortic valves or bovine pericardium has been limited because leaflet calcification (CALC) leads to valvular stenosis and/or regurgitation and subsequent valve failure. The present study was designed to test the hypothesis that preincubation of glutaraldehyde pretreated pericardial BPV leaflets in a solution containing the trivalent cation, AL , inhibits BPV calcification. BPV tissue was preincubated in 0.1 M ALCL3 for 24 hours (T = 25 degrees C), then subdermally implanted in rats (weanling, male, CD, Sprague-Dawley) for 21 days. Compared to the control group (tissue Ca++ = 143.81 +/- 25.49 micrograms/mg), calcification was markedly inhibited in the group pretreated with AL (tissue Ca++ = 4.1 +/- 1.03 micrograms/mg) after a 21 day implant. No adverse effects on rat growth or bone morphology were detected. It is concluded that the trivalent cation AL provided a marked anticalcification effect for BPV tissue implanted subdermally in the rat for 21 days.
mesh_terms: D000535:Aluminum / Q000627:therapeutic use*; D000077410:Aluminum Chloride; D017607:Aluminum Compounds; D000818:Animals; D001705:Bioprosthesis / Q000009:adverse effects; D002114:Calcinosis / Q000209:etiology / Q000517:prevention & control*; D002417:Cattle; D002712:Chlorides / Q000627:therapeutic use*; D005976:Glutaral; D006350:Heart Valve Prosthesis; D008297:Male; D010496:Pericardium; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D017607:Aluminum Compounds; D002712:Chlorides; D000077410:Aluminum Chloride; D000535:Aluminum; D005976:Glutaral