Endogenous inhibitor of the ADP-ribosylation of (a) G-protein(s) as catalyzed by pertussis toxin is present in rat liver.
The inhibitor activity of the ADP-ribosylation of (a) G-protein(s) as catalyzed by pertussis toxin was found in the membrane extract of rat liver. The inhibitor activity was found in the fractions of DEAE-Sephacel column chromatography at 50-120 mM NaCl. The inhibitor activity is not due to the degradation of NAD nor to the reverse reaction of pertussis toxin (removal of incorporated ADP-ribose). The present result suggests the presence of an endogenous inhibitor of the ADP-ribosylation reaction of (a) G-protein(s).
D000246:Adenosine Diphosphate Ribose / Q000378:metabolism*; D000818:Animals; D002462:Cell Membrane / Q000201:enzymology; D004591:Electrophoresis, Polyacrylamide Gel; D019204:GTP-Binding Proteins / Q000378:metabolism*; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008297:Male; D009243:NAD / Q000378:metabolism; D037342:Pertussis Toxin; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D051381:Rats; D011919:Rats, Inbred Strains; D010566:Virulence Factors, Bordetella / Q000378:metabolism*
D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D010566:Virulence Factors, Bordetella; D009243:NAD; D000246:Adenosine Diphosphate Ribose; D037342:Pertussis Toxin; D019204:GTP-Binding Proteins