title: Transmission of Akabane virus from the ewe to the early fetus (32 to 53 days).
abstract: The role of the placental junction in AKA virus infection in the ewe was examined during the time when the chorionic villi were first becoming firmly attached to the maternal caruncles. The studies were made over 21 days covering the period between 32 and 53 days of pregnancy. Viral tropism in the fetal membranes and tissues of the fetuses was identified by virus isolation and immuno-fluorescence studies. Areas of virus replication were noted from 24 h post-inoculation in the fetal membranes and persisted in these tissues throughout the experiment. Viral antigen was first detected in the fetus from day 5 post-inoculation by virus isolation and immuno-fluorescence. From this time on, viral activity increased in specific areas of the fetus, notably in the brain and, to a lesser extent, the skeletal muscles. Gross pathological changes occurred in the fetuses between day 14 and day 21 post-inoculation (46 to 53 days gestation). Despite the relatively high titres of AKA virus present in the placental tissues and the developmental changes occurring in the fetus due to the virus, the placental junction continued to carry out its physiological function of maintaining pregnancy.
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D005260:Female; D005315:Fetal Diseases / Q000473:pathology / Q000635:transmission / Q000662:veterinary*; D005333:Fetus / Q000382:microbiology; D005455:Fluorescent Antibody Technique; D010920:Placenta / Q000382:microbiology; D011247:Pregnancy; D012756:Sheep / Q000196:embryology / Q000382:microbiology; D012757:Sheep Diseases / Q000276:immunology / Q000473:pathology / Q000635:transmission*; D014777:Virus Diseases / Q000473:pathology / Q000635:transmission / Q000662:veterinary*; D018406:Virus Physiological Phenomena; D014780:Viruses / Q000302:isolation & purification
chemical_list: D000956:Antigens, Viral