Modulation of urokinase-type plasminogen activator messenger RNA levels in human synovial fibroblasts by interleukin-1, retinoic acid, and a glucocorticoid.
Previous studies have shown that mononuclear cell-conditioned medium (MCCM), interleukin-1 (IL-1), and all-trans-retinoic acid rapidly stimulate, while glucocorticoids lower, the urokinase-type plasminogen activator (u-PA) activity of human synovial fibroblast-like cells. It is now reported that MCCM, recombinant human IL-1 alpha (rHuIL-1 alpha), rHuIL-1 beta, and all-trans-retinoic acid elevate the u-PA messenger RNA (mRNA) levels to a steady-state value within 2 hours, while dexamethasone (10(-7)M) inhibits this increase. For both situations, when the u-PA activity is either stimulated or reduced, the changes in the u-PA mRNA levels parallel the changes in the u-PA activity, and it is suggested that modulation of gene transcription plays an important role.
D002478:Cells, Cultured; D003470:Culture Media; D003907:Dexamethasone / Q000494:pharmacology*; D004792:Enzyme Precursors / Q000235:genetics*; D005347:Fibroblasts / Q000187:drug effects; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology*; D010960:Plasminogen Activators / Q000235:genetics*; D012333:RNA, Messenger / Q000187:drug effects*; D011994:Recombinant Proteins / Q000494:pharmacology; D013583:Synovial Membrane / Q000166:cytology*; D013997:Time Factors; D014158:Transcription, Genetic / Q000187:drug effects; D014212:Tretinoin / Q000494:pharmacology*; D014568:Urokinase-Type Plasminogen Activator / Q000235:genetics*
D003470:Culture Media; D004792:Enzyme Precursors; D007375:Interleukin-1; D012333:RNA, Messenger; D011994:Recombinant Proteins; D014212:Tretinoin; D003907:Dexamethasone; D010960:Plasminogen Activators; D014568:Urokinase-Type Plasminogen Activator