Partial characterization of the enzymatic activity associated with the binary toxin (type C2) produced by Clostridium botulinum.
Clostridium botulinum produces a binary toxin that possesses a heavy chain (approximately 100,000 daltons) and a light chain (approximately 50,000 daltons). The heavy chain is a binding component that directs the toxin to vulnerable cells, and the light chain is an enzyme that has mono(ADP-ribosyl)ating activity. A number of experiments have been done to help characterize the enzymatic activity of the toxin. The data reveal that the enzyme has a pH optimum within the range of 7.0 to 8.0. It is not inhibited or stimulated by physiological concentrations of sodium, potassium, calcium, or magnesium. The enzyme is inhibited by high concentrations of salt, however, as well as high concentrations of nicotinamide, thymidine, theophylline, and histamine; and it is stimulated by histone and lysolecithin. Boiling irreversibly denatures the light chain of the toxin, but denaturation caused by guanidine and urea is substantially reversible. Enzymatic activity is not altered by short exposure to lysosomal proteases, including cathepsin B, cathepsin H, dipeptidyl aminopeptidase, and catheptic carboxypeptidase B.
D036002:ADP Ribose Transferases; D001426:Bacterial Proteins; D001427:Bacterial Toxins / Q000032:analysis*; D001905:Botulinum Toxins / Q000378:metabolism; D003014:Clostridium botulinum / Q000201:enzymology* / Q000276:immunology*; D004789:Enzyme Activation / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D008247:Lysosomes / Q000201:enzymology; D010430:Pentosyltransferases / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000378:metabolism*; D010447:Peptide Hydrolases / Q000494:pharmacology; D012492:Salts / Q000494:pharmacology; D014121:Toxoids / Q000378:metabolism*
D001426:Bacterial Proteins; D001427:Bacterial Toxins; C035415:Clostridium botulinum toxoid; D012492:Salts; D014121:Toxoids; C031815:toxB protein, Clostridium difficile; D036002:ADP Ribose Transferases; D010430:Pentosyltransferases; D010447:Peptide Hydrolases; D001905:Botulinum Toxins