Enzyme immunoassay for the detection of Giardia lamblia.
An enzyme immunoassay (EIA) was developed for direct detection of Giardia lamblia antigens in fecal specimens. The EIA was evaluated by testing specimens from 1,331 subjects in the USA and Egypt. For the 353 specimens from human subjects in the USA there was a 97% overall agreement between the results of the EIA and direct microscopic examination, yielding a sensitivity and specificity of 92% and 99% respectively. Due to adverse field conditions the EIA did not perform as well in the specimens collected and tested in Egypt. The sensitivity and specificity for 585 human specimens from Egypt were 74% and 97% respectively.
D000818:Animals; D000953:Antigens, Protozoan / Q000032:analysis*; D004534:Egypt; D005243:Feces / Q000469:parasitology; D005872:Giardia / Q000276:immunology*; D005873:Giardiasis / Q000175:diagnosis*; D006801:Humans; D007124:Immunoenzyme Techniques; D011237:Predictive Value of Tests; D014481:United States
D000953:Antigens, Protozoan