ELISA measurement of IgG subclass production in culture supernatants using monoclonal antibodies.
Specific and sensitive ELISA to quantitate the human IgG subclasses in cell culture supernatants are described. These assays detect a minimum of 5 ng/ml IgG1, 90 ng/ml IgG2, 8 ng/ml IgG3 and 8 ng/ml IgG4 and can generally measure IgG subclasses in lymphocyte cultures containing a minimum of 200 ng/ml of total IgG. The isotype specificity of these ELISA is demonstrated and each individual ELISA shown to react with a number of paraproteins of the relevant subclass independently of their light chain type or their (major Caucasian) allotype. These assays have been used to determine the IgG subclass response of normal human lymphocytes to pokeweed mitogen in vitro.
D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D003470:Culture Media / Q000032:analysis; D004306:Dose-Response Relationship, Immunologic; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D006801:Humans; D007126:Immunoglobulin Allotypes / Q000276:immunology; D007074:Immunoglobulin G / Q000032:analysis*; D007132:Immunoglobulin Isotypes / Q000032:analysis*; D007147:Immunoglobulin Light Chains / Q000276:immunology; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology; D008297:Male; D010266:Paraproteins / Q000032:analysis
D000911:Antibodies, Monoclonal; D003470:Culture Media; D007126:Immunoglobulin Allotypes; D007074:Immunoglobulin G; D007132:Immunoglobulin Isotypes; D007147:Immunoglobulin Light Chains; D010266:Paraproteins