Phosphate dilution lowers net phosphatidyl choline synthesis in lymphocytes.
The rate of net phosphatidyl choline (PC) synthesis in lymphocytes was evaluated, under appropriate conditions, in terms of [32P]phosphate incorporation. Phosphate-diluted and normal media were compared, with the cells being stimulated by concanavalin A or not. In either case, incubation in phosphate-diluted medium lowered net PC synthesis. In concanavalin A-stimulated cells, phosphate dilution also abolished the stimulation effect on net PC synthesis in G1. Nevertheless, these changes did not inhibit blastogenic transformation of cells.
D002478:Cells, Cultured; D003208:Concanavalin A; D004261:DNA Replication / Q000187:drug effects; D006801:Humans; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology / Q000378:metabolism*; D010710:Phosphates / Q000097:blood* / Q000494:pharmacology; D010713:Phosphatidylcholines / Q000096:biosynthesis / Q000097:blood*; D010761:Phosphorus Radioisotopes
D010710:Phosphates; D010713:Phosphatidylcholines; D010761:Phosphorus Radioisotopes; D003208:Concanavalin A