title: Selective inhibition of free arachidonic acid production in activated alveolar macrophages by calmodulin antagonists.
abstract: The effect of calmodulin antagonists on the amounts of free fatty acids produced by rabbit alveolar macrophages was determined by fluorometric high-performance liquid chromatography. Opsonized zymosan-induced arachidonic acid production was dramatically suppressed in the presence of W-7 and trifluoperazine without an effect on the production of other fatty acids. Calmodulin antagonists inhibited phospholipase A and abolished the release of arachidonic acid from phospholipids. The present results suggest that a zymosan-sensitive pool of 20:4, which is different from that of other fatty acids, is present in macrophages and that calmodulin antagonists selectively inhibit phospholipase A, which preferentially degrades phospholipids with 20:4.
mesh_terms: D000098:Acetophenones / Q000494:pharmacology; D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism*; D002147:Calmodulin / Q000037:antagonists & inhibitors*; D002746:Chlorpromazine / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D003571:Cytochalasin B / Q000494:pharmacology; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000378:metabolism; D008262:Macrophage Activation; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D019301:Oleic Acid; D009829:Oleic Acids / Q000378:metabolism; D009895:Opsonin Proteins; D010713:Phosphatidylcholines / Q000378:metabolism; D010716:Phosphatidylinositols / Q000378:metabolism; D010741:Phospholipases A / Q000037:antagonists & inhibitors; D011650:Pulmonary Alveoli / Q000166:cytology*; D011796:Quinacrine / Q000494:pharmacology; D011817:Rabbits; D013449:Sulfonamides / Q000494:pharmacology; D014268:Trifluoperazine / Q000494:pharmacology; D015054:Zymosan / Q000494:pharmacology
chemical_list: D000098:Acetophenones; D001095:Arachidonic Acids; D002147:Calmodulin; D008041:Linoleic Acids; D009829:Oleic Acids; D009895:Opsonin Proteins; D010713:Phosphatidylcholines; D010716:Phosphatidylinositols; D013449:Sulfonamides; D014268:Trifluoperazine; D016718:Arachidonic Acid; D019301:Oleic Acid; D003571:Cytochalasin B; C017967:W 7; D015054:Zymosan; D019787:Linoleic Acid; D010741:Phospholipases A; D011796:Quinacrine; C006821:4-bromophenacyl bromide; D002746:Chlorpromazine