Distribution of type I, II, III and V in the pepsin solubilized collagens in bovine menisci.
The inner one-third (IM) of both lateral and medial menisci resembles hyaline cartilage, both in gross appearance and histological examination, while the outer two-thirds (OM) is fibrocartilaginous in appearance. Collagen was extracted with pepsin, purified with anion and cation exchange column chromatographies and examined by differential salt precipitation, cyanogen bromide-peptide analysis and SDS gel electrophoresis. IM constitutes approximately 10% of the wet weight of whole meniscus, is made up of 70% collagen of which 34% is pepsin soluble. IM is composed of 60% type II and 40% type I collagen. OM is made up of 80% collagen of which 17% is pepsin soluble. The predominant collagen of OM is type I with a trace amount of types III and V (less than 1%).
D000818:Animals; D002358:Cartilage, Articular / Q000032:analysis; D002417:Cattle; D011232:Chemical Precipitation; D002852:Chromatography, Ion Exchange; D003094:Collagen / Q000032:analysis*; D003488:Cyanogen Bromide; D004591:Electrophoresis, Polyacrylamide Gel; D008592:Menisci, Tibial / Q000032:analysis*; D010434:Pepsin A; D012867:Skin / Q000032:analysis; D012995:Solubility
D003094:Collagen; D010434:Pepsin A; D003488:Cyanogen Bromide