Immunochemical characterization of human red cell acid phosphatase isozymes.
An immunological study was performed on human red cell acid phosphatase (ACP1) isozymes encoded by different alleles, each of which is expressed as an electrophoretically fast (f) isozyme and a slow (s) isozyme. These isozymes reacted as two immunochemically different groups. Allele-specific reactions were not detected between either the f isozymes or the s isozymes. Quantitation of ACP1 isozymes in red cells by crossed immunoelectrophoresis revealed a phenotype-dependent variation in the concentration of isozyme protein. A simple gene dosage effect was indicated and the ordering of the ACP1 alleles (ACP1*A less than ACP1*B less than ACP1*C less than ACP1*E) was identical to that found for enzyme activity levels. Also, an allele effect on the proportion between s and f isozymes (s/f) was observed; the ordering here was ACP1*B less than ACP1*A less than ACP1*C, which is the same as that reported for the susceptibility to modulation with purines. These variations in isozyme protein levels appear to account for the phenotypic differences in the intensity of the isozyme bands, when activity-stained after electrophoresis, and in the red cell enzyme activity levels. Investigation of two carriers of a Null allele showed no evidence of an aberrant protein product, and half-normal concentrations of enzyme protein were observed in the red cells of these individuals.
D000135:Acid Phosphatase / Q000097:blood* / Q000235:genetics; D000483:Alleles; D004592:Electrophoresis, Starch Gel; D004912:Erythrocytes / Q000201:enzymology*; D006580:Genetic Carrier Screening; D006801:Humans; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D007123:Immunoelectrophoresis, Two-Dimensional; D007527:Isoenzymes / Q000097:blood* / Q000235:genetics; D012016:Reference Values
D007527:Isoenzymes; D000135:Acid Phosphatase