A nondestructive method for the measurement of radioactive 14CO2 in blood.
A method for estimation of 14CO2 present in blood and tissular samples is described. It is basically based on the introduction of large amounts of a gas mixture (95% O2, 5% CO2) in the samples which serves to remove the CO2 label by gas dilution. The gas phase is later captured in scintillation vials containing an organic-soluble base that retains the carbon label. The results obtained by means of this methodology show much better recoveries for blood samples than those obtained when the classic acid-diffusion method is used. In addition, it is a very fast procedure which does not alter the pH or protein integrity of the biological sample.
D000818:Animals; D002245:Carbon Dioxide / Q000032:analysis / Q000097:blood*; D002250:Carbon Radioisotopes; D006863:Hydrogen-Ion Concentration; D007553:Isotope Labeling; D008099:Liver / Q000032:analysis; D051381:Rats; D012588:Scintillation Counting
D002250:Carbon Radioisotopes; D002245:Carbon Dioxide