Extraction of protective components of Rickettsia rickettsii with n-octyl-beta-D-glucopyranoside.
Two Rickettsia rickettsii proteins, with apparent molecular masses of 120 and 155 kilodaltons, have been implicated as important antigens in immunity to Rocky Mountain spotted fever. An n-octyl-beta-D-glucopyranoside-soluble fraction of R. rickettsii was found to be enriched for these proteins along with others. Vaccination of mice with the detergent-soluble fraction protected them against death caused by R. rickettsii and protected guinea pigs from developing Rocky Mountain spotted fever after being challenged with viable R. rickettsii.
D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D005960:Glucosides; D006027:Glycosides; D006168:Guinea Pigs; D008297:Male; D051379:Mice; D012284:Rickettsia rickettsii / Q000276:immunology*; D012290:Rickettsial Vaccines; D012373:Rocky Mountain Spotted Fever / Q000517:prevention & control*; D014612:Vaccines
D000942:Antigens, Bacterial; D005960:Glucosides; D006027:Glycosides; D012290:Rickettsial Vaccines; D014612:Vaccines; C018619:octyl-beta-D-glucoside