Generalized seizures deplete brain energy reserves in normoxemic newborn monkeys.
The cerebral metabolic response to bicuculline (BC)-induced status epilepticus (SE) was studied in two-week-old ketamine-anesthetized marmoset monkeys. During 30-min clonic seizures, mean blood pressure, plasma glucose and paO2 did not decrease and plasma lactate doubled. Brains were funnel-frozen and punch biopsies of frontoparietal cortex, temporal cortex and thalamus were analyzed for ATP, phosphocreatine (PCr), glucose and lactate. There were marked reductions of ATP (to 56-77% of controls), PCr (to 23-28% of controls) and glucose (to 1-4% of controls), and lactate increased 3- to 6-fold in seizure animals. NADH fluorescence increased during seizures in cerebral cortex, thalamus, amygdaloid nuclei, hippocampus, posterior striatum and hemispheric white matter. This suggests a reduced tissue redox state in these regions and is correlated with the high energy phosphate depletion and elevated lactate in cortex and thalamus. Our results demonstrate a significant depletion of energy reserves and glucose in cerebral cortex and thalamus during neonatal seizures in the absence of adverse systemic factors. These seizure-induced metabolic changes in brain could have adverse long-term effects on brain development and function.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn / Q000502:physiology*; D001640:Bicuculline; D001921:Brain / Q000378:metabolism* / Q000503:physiopathology; D002143:Callitrichinae / Q000502:physiology*; D004734:Energy Metabolism; D005947:Glucose / Q000378:metabolism; D007773:Lactates / Q000378:metabolism; D019344:Lactic Acid; D009243:NAD / Q000378:metabolism; D010725:Phosphocreatine / Q000378:metabolism; D012640:Seizures / Q000139:chemically induced / Q000378:metabolism*
D007773:Lactates; D010725:Phosphocreatine; D009243:NAD; D019344:Lactic Acid; D000255:Adenosine Triphosphate; D005947:Glucose; D001640:Bicuculline