[Mechanisms of polyclonal tolerance induced by lipopolysaccharide and cyclophosphamide].
The treatment of recipient mice with LPS from S. marcescens followed by the injection of CY 48 h later inhibited a subsequent antibody production against unrelated antigen (SRBC) and polyclonal mitogen (LPS from Br. abortus). Such a reactivity persisted for 2-3 weeks after treatment. It was shown that the number of Ig+ cells in the spleens of treated mice was decreased, while the population of spleen Thy-1.2+ cells remained unaltered. Cell-cooperative test revealed that the function of B cells, but not T cells, was inhibited by the treatment. There were no changes in DTH response to SRBC. Thus, a subsequent treatment of mice with LPS and CY led to B-cell deficiency. The nature of this phenomenon is presumably the same as the nature of CY-induced antigen-specific immunological tolerance.
D000818:Animals; D000921:Antibody-Producing Cells / Q000187:drug effects / Q000276:immunology; D002003:Brucella abortus; D003520:Cyclophosphamide / Q000494:pharmacology*; D003601:Cytotoxicity Tests, Immunologic; D004912:Erythrocytes / Q000276:immunology; D006968:Hypersensitivity, Delayed / Q000276:immunology; D007108:Immune Tolerance / Q000187:drug effects*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008211:Lymphocyte Cooperation / Q000187:drug effects; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D012706:Serratia marcescens; D013997:Time Factors
D008070:Lipopolysaccharides; D003520:Cyclophosphamide
Mekhanizmy poliklonal'noĭ tolerantnosti, indutsirovannoĭ lipopolisakharidom i tsiklofosfamidom.