Cloning of DNA fragments containing Streptomyces promoter activity.
The thiostrepton-resistance gene is expressed in Streptomyces jumonjinensis [16]-8 SANK 61185 carrying the plasmid pIJ702 but not the tyrosinase gene. We have isolated DNA fragments from various streptomycetes that restore expression of the tyrosinase gene on pIJ702 in this organism. The nucleotide sequences of two of these DNA fragments show that they contain putative ribosome binding sites and- 10 regions of possible promoters.
D001483:Base Sequence; D004156:Catechol Oxidase / Q000235:genetics*; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000032:analysis*; D014442:Monophenol Monooxygenase / Q000235:genetics*; D010957:Plasmids; D011401:Promoter Regions, Genetic; D013302:Streptomyces / Q000235:genetics*
D004269:DNA, Bacterial; D004156:Catechol Oxidase; D014442:Monophenol Monooxygenase