Phosphatidylinositol-specific phospholipase D: a pathway for generation of a second messenger.
We have found a phospholipase D activity in the postnuclear fraction of human neutrophils which is stimulated by incubation of cells with the calcium ionophore A23187. The phospholipase D activity was assessed by both phosphatidate formation and free inositol release from phosphatidylinositol substrate. The phospholipase D activity shows an optimum pH of 7.5 and hydrolyzes specifically phosphatidylinositol. These results suggest that this phosphatidylinositol-specific phospholipase D can play a role in cell activating process.
D000001:Calcimycin / Q000494:pharmacology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D010713:Phosphatidylcholines / Q000097:blood; D010716:Phosphatidylinositols / Q000097:blood*; D010739:Phospholipase D / Q000097:blood*; D010740:Phospholipases / Q000097:blood*; D010738:Type C Phospholipases / Q000097:blood
D010713:Phosphatidylcholines; D010716:Phosphatidylinositols; D000001:Calcimycin; D010740:Phospholipases; D010738:Type C Phospholipases; D010739:Phospholipase D