Colchicine activates cell cycle-dependent genes in growth-arrested rat 3Y1 cells.
When growth-arrested 3Y1 cells (Fischer rat fibroblasts) were exposed to 3 X 10(-5) M colchicine, they entered S phase after a 12-h lag period which is the same as that in serum-stimulated cells. The expression of genes such as c-fos, c-myc, JE, KC, ornithine decarboxylase, and histone H3, analyzed by Northern blotting, increased in a cell-cycle dependent manner after colchicine treatment. The increased level of mRNAs was much smaller in colchicine-stimulated cells than in serum-stimulated cells, corresponding to the lower frequency of the former cells entering S phase. The course of the prereplicative phase seems to be similar in terms of the expression of cell cycle-dependent genes in cells stimulated with colchicine and in those stimulated with serum.
D000818:Animals; D002453:Cell Cycle / Q000187:drug effects*; D003078:Colchicine / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects*; D006657:Histones / Q000235:genetics; D009955:Ornithine Decarboxylase / Q000235:genetics; D011518:Proto-Oncogene Proteins / Q000235:genetics; D016760:Proto-Oncogene Proteins c-fos; D016271:Proto-Oncogene Proteins c-myc; D051381:Rats
D006657:Histones; D011518:Proto-Oncogene Proteins; D016760:Proto-Oncogene Proteins c-fos; D016271:Proto-Oncogene Proteins c-myc; D009955:Ornithine Decarboxylase; D003078:Colchicine