title: A crotoxin homolog from the venom of the Uracoan rattlesnake (Crotalus vegrandis).
abstract: A major protein toxin from the venom of Crotalus vegrandis was examined by gel filtration, anion-exchange chromatography, and SDS polyacrylamide gel electrophoresis. The toxin was separated into several isoforms by ion-exchange chromatography and spontaneously dissociated into free acidic and basic subunits, mimicking the behavior of crotoxin. Rabbit antisera raised against crotoxin reacted strongly in enzyme-linked immunosorbent assays with the intact C. vegrandis toxin isoforms and their basic subunits, and formed precipitin lines of identity with intact crotoxin in double immunodiffusion gels. These results indicate that vegrandis toxin is strongly homologous with crotoxin from the venom of Crotalus durissus terrificus.
mesh_terms: D000818:Animals; D003435:Crotalid Venoms / Q000032:analysis* / Q000276:immunology / Q000633:toxicity; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005779:Immunodiffusion; D051379:Mice; D011506:Proteins / Q000032:analysis; D012967:Sodium Dodecyl Sulfate; D014118:Toxins, Biological / Q000032:analysis* / Q000276:immunology / Q000633:toxicity
chemical_list: D003435:Crotalid Venoms; D011506:Proteins; D014118:Toxins, Biological; D012967:Sodium Dodecyl Sulfate