Chromosomal assignment of the murine Gi alpha and Gs alpha genes. Implications for the obese mouse.
The G protein family of transmembrane signaling molecules includes Gs and Gi, the stimulatory and inhibitory regulators of adenylate cyclase. These and other characterized G proteins are comprised of beta, gamma, and alpha chains, the latter being the most variable among the proteins and thus serving to distinguish them. Previous results (Begin-Heick, N. (1985) J. Biol. Chem. 260, 6187-6193) suggested that the autosomal recessive mouse mutation obese (ob), which results in an abnormal response of adipose tissue to lipolytic hormones, is due to a defect in the gene coding for the alpha chain of Gi. In order to test this hypothesis we used a cloned cDNA probe representing murine Gi alpha mRNA in conjunction with a panel of Chinese hamster-mouse somatic cell hybrids segregating mouse chromosomes to map the Gi alpha gene in the mouse. In addition, we used a cDNA probe representing the murine Gs alpha gene to a specific mouse chromosome. Our results indicate that the Gi alpha locus maps to mouse chromosome 9, while Gs alpha is localized to region 2E1-2H3 of mouse chromosome 2. Localization of the Gi alpha gene to chromosome 9 excludes this gene as a site of the ob mutation, since the ob locus maps to chromosome 6. Furthermore, our findings indicate that certain members of the murine G protein alpha gene family have dispersed to different chromosomes since diverging from a common ancestral gene.
D000818:Animals; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D019204:GTP-Binding Proteins / Q000235:genetics*; D005796:Genes; D006822:Hybrid Cells / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism; D051379:Mice; D008820:Mice, Obese / Q000235:genetics*; D009154:Mutation
D019204:GTP-Binding Proteins