Correlation of susceptibility and cytostatic factor-inducing activity of tumour cells to peritoneal macrophages. The role of concanavalin A-binding glycopeptides extracted from the tumour cell surface.
L929, 3T12-3, B16, 3LL, and YAC1 cells with cytostatic factor (CF)-inducing activity from Lactobacillus casei-elicited murine peritoneal macrophages (LCEPM) were susceptible to the cytostatic activity of LCEPM and to LCEPM-produced CF, but L1210, P388D1, and Colon 26 cells, which have no CF-inducing activity, were resistant to that of LCEPM and and to the CF. The resistance of P815 cells to that of LCEPM was stronger than that of 3T12-3 cells, but the CF-inducing activity of P815 cells was about 50% weaker than that of 3T12-3 cells. Release of CF from LCEPM was also caused by heat-killed (100 degrees C, 10 min) 3T12-3 or P815 cells, and this release was inhibited by D-mannose. The CF-inducing activity of heat-killed 3T12-3 or P815 cells was reduced by mild trypsin digestion (37 degrees C for 10 min). A D-mannose-containing glycopeptide or glycoprotein (GP) was separated from 3T12-3 or P815 cells by concanavalin A (Con A) or wheat germ agglutinin (WGA) affinity chromatography. The CF were released from LCEPM by stimulation with the Con A-binding GP of the tumour cells, but the WGA-binding GP had little activity. It is suggested that tumour cells with CF-inducing activity may be susceptible to the cytostatic activity of LCEPM, and those without CF-inducing activity may be resistant to the cytostatic activity of LCEPM and the release of CF from activated macrophages may be caused by the Con A-binding GP of the tumour cell surface.
D000818:Animals; D003603:Cytotoxins / Q000096:biosynthesis* / Q000502:physiology; D006020:Glycopeptides / Q000502:physiology*; D006358:Hot Temperature; D007113:Immunity, Innate; D007780:Lacticaseibacillus casei / Q000276:immunology; D008262:Macrophage Activation; D008297:Male; D008562:Membrane Glycoproteins / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009363:Neoplasm Proteins / Q000502:physiology*; D010529:Peritoneal Cavity / Q000166:cytology; D011506:Proteins; D011952:Receptors, Concanavalin A / Q000502:physiology*; D014357:Trypsin; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000502:physiology; D014909:Wheat Germ Agglutinins / Q000502:physiology
D003603:Cytotoxins; D006020:Glycopeptides; D008562:Membrane Glycoproteins; D009363:Neoplasm Proteins; D011506:Proteins; D011952:Receptors, Concanavalin A; D014409:Tumor Necrosis Factor-alpha; D014909:Wheat Germ Agglutinins; C027774:concanavalin A-binding glycoproteins; D014357:Trypsin