title: High sensitivity and specificity assay for detection of leukemia/lymphoma cells in human bone marrow.
abstract: An assay of high sensitivity and specificity for detection of residual malignant cells in remission bone marrow from patients with acute lymphoblastic leukemia (ALL) and non-Hodgkin's lymphoma (NHL) has been developed. The assay combines an immunoselection step with immunoglobulin gene rearrangement analysis by high specific-activity DNA probes. In experimental conditions, less than one contaminating tumor cell out of 1000 bone marrow cells was detected. A bone marrow contamination was detected in morphologically negative bone marrow specimens from two intermediate-grade non-Hodgkin's lymphoma patients. This method can be of value in determining a true remission status in high-risk ALL and NHL patients.
mesh_terms: D000911:Antibodies, Monoclonal; D001853:Bone Marrow / Q000473:pathology*; D001856:Bone Marrow Examination / Q000379:methods*; D002051:Burkitt Lymphoma / Q000235:genetics / Q000473:pathology; D002469:Cell Separation; D004273:DNA, Neoplasm / Q000032:analysis*; D005434:Flow Cytometry; D005803:Genes, Immunoglobulin; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D007945:Leukemia, Lymphoid / Q000473:pathology*; D008228:Lymphoma, Non-Hodgkin / Q000235:genetics / Q000473:pathology*; D014411:Neoplastic Stem Cells / Q000032:analysis*; D009693:Nucleic Acid Hybridization; D014407:Tumor Cells, Cultured / Q000032:analysis
chemical_list: D000911:Antibodies, Monoclonal; D004273:DNA, Neoplasm; D007143:Immunoglobulin Heavy Chains