Pyrazinamidase activity of Mycobacterium tuberculosis--a test of sensitivity to pyrazinamide.
Pyrazinamidase activity has been found to correlate with pyrazinamide sensitivity in strains of Mycobacterium tuberculosis. In vitro sensitivity to pyrazinamide in acidified Löwenstein-Jensen medium, and pyrazinamidase activity by the Wayne method, were determined in 378 clinical isolates of M. tuberculosis. A close correlation was observed between the results of both tests. This method of detecting pyrazinamidase activity was found to be a rapid, simple and reliable substitute for pyrazinamide sensitivity testing, and it overcomes the difficulty of growing M. tuberculosis at pH 5.5, as required in the standard method.
D000581:Amidohydrolases / Q000378:metabolism*; D008826:Microbial Sensitivity Tests; D009169:Mycobacterium tuberculosis / Q000187:drug effects / Q000201:enzymology*; D011718:Pyrazinamide / Q000494:pharmacology*
D011718:Pyrazinamide; D000581:Amidohydrolases; C022490:pyrazinamide deamidase