Further studies on chromosome 15 trisomy in murine T-cell lymphomas: mapping of the relevant chromosome segment.
Trisomy 15 is the most common chromosomal aberration in murine T-cell lymphomas. The relevant chromosomal region responsible for the growth advantage of the 15-trisomic cell has not been defined. In order to map this region, we have induced thymic lymphomas by chemical carcinogens (DMBA or MNU) in mice with 2 different constitutional translocations, T(7;15)9H homozygotes and [T(7;15)9H X T(5;15)4Ad] FI hybrids. Twenty-two tumors developed in 90 carcinogen-treated mice. Among the 14 cytogenetically analyzed thymic lymphomas, 4 were diploid and 5 were aneuploid, with no chromosome-15-associated changes. Five lymphomas showed partial duplication of chromosome 15. Four of them have duplicated the segment distal to the C/DI breakpoint of T9H, while the 5th carried an interstitial duplication of the D2 sub-band of the T(7;15) translocation chromosome. These findings suggest that the duplication of the D 2/3 region, known to contain the c-myc and the pvt-I genes (Banerjee et al., 1985), rather than other regions of chromosome 15, contributes to the development and/or progression of murine T-cell leukemias.
D015127:9,10-Dimethyl-1,2-benzanthracene; D000818:Animals; D002871:Chromosome Banding; D002874:Chromosome Mapping; D007621:Karyotyping; D008223:Lymphoma / Q000139:chemically induced / Q000235:genetics*; D008770:Methylnitrosourea; D051379:Mice; D008806:Mice, Inbred AKR; D013601:T-Lymphocytes; D014314:Trisomy
D015127:9,10-Dimethyl-1,2-benzanthracene; D008770:Methylnitrosourea