Misaminoacylation and transamidation are required for protein biosynthesis in Lactobacillus bulgaricus.
Aminoacylation studies with Lactobacillus bulgaricus show that this organism possesses glutamyl-tRNA synthetase activity; however, glutamyl-tRNA synthetase activity cannot be demonstrated. Instead, Glu-tRNAGln, which is formed by glutamyl-tRNA synthetase, is amidated by a specific amidotransferase to Gln-tRNAGln. The amide donor in this reaction is glutamine. Thus, Gln-tRNAGln in this organism is not formed by direct glutaminylation of tRNAGln, but instead by a pathway which involves misaminoacylation and transamidation.
D000577:Amides / Q000378:metabolism*; D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism*; D001426:Bacterial Proteins / Q000096:biosynthesis*; D005975:Glutamate-tRNA Ligase / Q000378:metabolism*; D005971:Glutamates / Q000378:metabolism; D005973:Glutamine / Q000378:metabolism; D007778:Lactobacillus / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D012346:RNA, Transfer, Amino Acyl / Q000378:metabolism*; D013997:Time Factors; D014166:Transferases / Q000378:metabolism*
D000577:Amides; D001426:Bacterial Proteins; D005971:Glutamates; D012346:RNA, Transfer, Amino Acyl; D005973:Glutamine; D014166:Transferases; D000604:Amino Acyl-tRNA Synthetases; D005975:Glutamate-tRNA Ligase