Effects of S-adenosylmethionine on human articular chondrocyte differentiation. An in vitro study.
The effect of S-adenosyl-L-methionine (SAMe) on human articular osteoarthritic chondrocytes was studied using a thick-layer culture model. Three SAMe concentrations were tested (1, 10, and 100 micrograms/ml). For 10 micrograms/ml, the most efficient dose, a significant rise in the incorporation levels of 35S-sulfate and 3H-serine was observed, as was as an increase in the hexuronic acid content. All the parameters seem to express a more active protein synthesis, particularly for proteoglycans. Under the same conditions, the proliferation rate of chondrocytes does not undergo important variations. These results point to a direct action on the cell metabolism but little is known concerning the mechanism involved.
D002358:Cartilage, Articular / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D002478:Cells, Cultured / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D006603:Hexuronic Acids / Q000378:metabolism; D006801:Humans; D011509:Proteoglycans / Q000096:biosynthesis; D012436:S-Adenosylmethionine / Q000008:administration & dosage / Q000494:pharmacology*; D012694:Serine / Q000378:metabolism; D013431:Sulfates / Q000378:metabolism
D006603:Hexuronic Acids; D011509:Proteoglycans; D013431:Sulfates; D012694:Serine; D012436:S-Adenosylmethionine