Homocitrate cures the NifV- phenotype in Klebsiella pneumoniae.
Dinitrogenase was isolated from a culture of a Klebsiella pneumoniae NifV- strain derepressed for nitrogenase in the presence of homocitrate. The enzyme isolated from this culture was identical to the wild-type dinitrogenase. These data provide in vivo evidence that the absence of homocitrate is responsible for the NifV- phenotype.
D017037:Dinitrogenase Reductase; D004794:Enzyme Repression; D005288:Ferredoxins / Q000378:metabolism; D005798:Genes, Bacterial; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D009154:Mutation; D009586:Nitrogen Fixation / Q000235:genetics*; D009591:Nitrogenase / Q000096:biosynthesis*; D010641:Phenotype; D014233:Tricarboxylic Acids / Q000494:pharmacology*
D017037:Dinitrogenase Reductase; D005288:Ferredoxins; D014233:Tricarboxylic Acids; C028143:homocitric acid; D009591:Nitrogenase