DNA damage and repair in Stylonychia lemnae (Ciliata, Protozoa).
Irradiation with X rays, UV irradiation after incorporation of bromodeoxyuridine (BU) into the DNA, and cis-platinum (cis-Pt) treatment each cause the loss of micronuclei of Stylonychia lemnae while the macronuclei are not severely affected. The abilities of both nuclei to repair DNA were investigated. Unscheduled DNA synthesis could not be demonstrated after X-ray irradiation, but it was found after treatment with BU/UV and cis-Pt in macro- and micronuclei. The extent of the repair process in the micro- and macronuclei was alike, as indicated by grain counts of [6-3H]thymidine-treated cells. One reason for the different sensitivity of both nuclei to DNA-damaging treatment may be the different number of gene copies in the macro- and micronuclei.
D000818:Animals; D001973:Bromodeoxyuridine / Q000494:pharmacology; D002467:Cell Nucleus / Q000187:drug effects / Q000528:radiation effects; D016798:Ciliophora / Q000187:drug effects / Q000235:genetics* / Q000528:radiation effects; D002945:Cisplatin / Q000494:pharmacology; D004247:DNA / Q000096:biosynthesis* / Q000187:drug effects / Q000528:radiation effects; D004249:DNA Damage; D004260:DNA Repair
D004247:DNA; D001973:Bromodeoxyuridine; D002945:Cisplatin