Expression of the enkephalin precursor gene in rat Sertoli cells. Regulation by follicle-stimulating hormone.
Searching for somatic cells expressing the preproenkephalin (A) gene in the testis, we have isolated Sertoli cells from the testes of 20-day-old rats. Cultured Sertoli cells contained a single species (about 1.5 kb) of preproenkephalin mRNA, and follicle-stimulating hormone (FSH) transiently increased the mRNA abundance to a maximum (about 30 molecules per cell) at 12 h. Various compounds that activate the cyclic AMP system in Sertoli cells similarly increased the abundance of preproenkephalin mRNA. Moreover, FSH increased intracellular Met-enkephalin immunoreactive peptides in Sertoli cells. Thus, the preproenkephalin gene expression in Sertoli cells is positively regulated by FSH through the cyclic AMP system.
D000199:Actins / Q000235:genetics; D000818:Animals; D002478:Cells, Cultured; D004744:Enkephalin, Methionine / Q000096:biosynthesis / Q000235:genetics; D004745:Enkephalins / Q000235:genetics*; D005640:Follicle Stimulating Hormone / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects*; D005796:Genes / Q000187:drug effects*; D007700:Kinetics; D008297:Male; D011498:Protein Precursors / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D012016:Reference Values; D012708:Sertoli Cells / Q000187:drug effects / Q000378:metabolism*; D014158:Transcription, Genetic / Q000187:drug effects*
D000199:Actins; D004745:Enkephalins; D011498:Protein Precursors; D012333:RNA, Messenger; D004744:Enkephalin, Methionine; D005640:Follicle Stimulating Hormone; C033660:preproenkephalin