[Adrenergic activation of the liberation of corticotropin-releasing hormone (CRH41) in the median eminence of unanesthetized rats].
To shed light on the persistent controversy concerning the role of central adrenergic systems in the corticotropic axis, we measured the effects of adrenaline on the release of IR-rCRH41 into a push-pull cannula stereotaxically implanted in the median eminence. Eight days before experimentation, the cannula was implanted, under deep anesthesia, at which time a standard cannula was additionally placed in the lateral ventricle (i.c.v.). The rats were not anesthetized during the main experiment. An i.c.v. injection of 5 microliter of solvent only slightly affected the release of CRH41, whereas an i.c.v. infusion of 1.4 micrograms adrenaline bitartrate under the same conditions led to an instantaneous, but short-lived, 8-fold rise in the CRH41 release above baseline. The data provide the first demonstration of a central stimulatory effect of adrenaline on the secretory activity of CRH41 producing neurons, in unanesthetized, free-moving rats.
D000818:Animals; D002404:Catheterization; D002552:Cerebral Ventricles; D003346:Corticotropin-Releasing Hormone / Q000378:metabolism*; D004837:Epinephrine / Q000008:administration & dosage / Q000494:pharmacology*; D007260:Infusion Pumps; D008297:Male; D008473:Median Eminence / Q000378:metabolism*; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains
D003346:Corticotropin-Releasing Hormone; D004837:Epinephrine
Activation adrénergique de la libération de la corticolibérine (CRH41) dans l'éminence médiane du rat non anesthésié.