title: Regional neurohypophysial and hypothalamic blood flow in rats during hypercapnia.
abstract: Regional cerebral blood flow (rCBF) was measured in the neurohypophysis and hypothalamus in normocapnic and hypercapnic rats using [14C]isopropyliodoamphetamine. Rats were surgically prepared using nitrous oxide and halothane and placed in plaster restraining casts. Hypercapnia was produced by increasing the fractional concentration of inspired CO2 (FICO2). rCBF in normocapnic rats was higher in the paraventricular nucleus, supraoptic nucleus, median eminence, and neural lobe than rates previously measured by use of diffusible tracers. During hypercapnia blood flow increased linearly with arterial PCO2 (PACO2) in all regions except the median eminence and neural lobe, which were not affected by hypercapnia. When rats were pretreated with phentolamine (1 mg/kg) to block the alpha-adrenergic receptors, blood flow in the median eminence and neural lobe increased significantly during hypercapnia. We conclude that blood flow in the cell bodies of the paraventricular nucleus and supraoptic nucleus is regulated differently during hypercapnia than blood flow in the nerve terminals in the median eminence and neural lobe. Furthermore, vasodilation produced by increased CO2 is offset by alpha-receptor stimulation in the median eminence and neural lobe.
mesh_terms: D000662:Amphetamines; D000818:Animals; D002245:Carbon Dioxide / Q000097:blood*; D002560:Cerebrovascular Circulation; D006935:Hypercapnia / Q000503:physiopathology*; D007030:Hypothalamo-Hypophyseal System / Q000098:blood supply*; D007457:Iodine Radioisotopes; D020367:Iofetamine; D008297:Male; D010313:Partial Pressure; D010646:Phentolamine / Q000494:pharmacology; D051381:Rats; D012016:Reference Values; D012039:Regional Blood Flow / Q000187:drug effects
chemical_list: D000662:Amphetamines; D007457:Iodine Radioisotopes; D002245:Carbon Dioxide; D020367:Iofetamine; D010646:Phentolamine