[Interaction of chondroitin sulfate with enzymes].
Chondroitin sulphate forms noncovalent electrostatic biocatalyst-glycosaminoglycan complexes in the solutions of enzymes. Chondroitin sulphate also interacts with enzymes developing complexes after carbodiimide activation of its carboxylic groups. Relatively low-molecular weight of biocatalysts (chymotrypsin, superoxide dismutase) forms stable noncovalent conjugates with the additional interaction as compared with electrostatic complexes. High-molecular weight of enzymes (acid phosphatase) develops covalent conjugates. Chondroitin sulphate is proposed for covalent binding of large proteins and multi-enzymatic complexes to obtain their stabilized derivatives for medical application.
D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D002384:Catalysis; D002807:Chondroitin / Q000031:analogs & derivatives*; D002809:Chondroitin Sulfates / Q000378:metabolism*; D002850:Chromatography, Gel; D002918:Chymotrypsin / Q000378:metabolism; D004798:Enzymes / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D051381:Rats; D013482:Superoxide Dismutase / Q000378:metabolism
D004798:Enzymes; D002807:Chondroitin; D002809:Chondroitin Sulfates; D013482:Superoxide Dismutase; D000135:Acid Phosphatase; D002918:Chymotrypsin
Vzaimodeĭstvie khondroitinsul'fata s fermentami.