Crystallization and preliminary X-ray study of pig lens aldose reductase.
Crystals of pig lens aldose reductase have been grown from polyethylene glycol solutions at pH 6.2 and analysed by X-ray diffraction. Two crystal forms were obtained. The first belongs to space group P1 with unit cell dimensions a = 81.3 A, b = 85.9 A, c = 56.6 A, alpha = 102.3 degrees, beta = 103.3 degrees, gamma = 79.0 degrees, with four molecules in the unit cell related by a 222 non-crystallographic symmetry. The second crystal form is hexagonal. The space group is P6(2)22 with a = b = 101 A, c = 257 A and two molecules in the asymmetric unit. Both forms are suitable for X-ray structure analysis to better than 3 A resolution.
D000449:Aldehyde Reductase; D000818:Animals; D003461:Crystallography; D007908:Lens, Crystalline / Q000201:enzymology*; D013401:Sugar Alcohol Dehydrogenases; D013552:Swine / Q000378:metabolism*; D014961:X-Ray Diffraction
D013401:Sugar Alcohol Dehydrogenases; D000449:Aldehyde Reductase