Promutagen activation by freshly isolated and cryopreserved rat hepatocytes.
The metabolic activation of promutagens by freshly isolated and cryopreserved rat hepatocytes was compared using the Chinese hamster ovary/hypoxanthine-guanine phosphoribosyltransferase assay (CHO/HGPRT). Cryopreserved rat hepatocytes were equivalent to freshly isolated hepatocytes in their ability to metabolize dimethylbenz(a)anthracene (DMBA) and dimethylnitrosamine (DMN) to active mutagens. Similar dose-response curves were observed using either freshly isolated or cryopreserved hepatocytes as activating systems after treatment with DMBA (0.1-1 micrograms/ml) and DMN (0.075-0.6 mg/ml). Our results suggest that cryopreserved hepatocytes are similar to freshly isolated hepatocytes as an experimental system for studies on promutagen activation.
D015127:9,10-Dimethyl-1,2-benzanthracene / Q000378:metabolism*; D000818:Animals; D002470:Cell Survival; D004128:Dimethylnitrosamine / Q000378:metabolism*; D005260:Female; D005615:Freezing; D008099:Liver / Q000166:cytology* / Q000378:metabolism; D009153:Mutagens / Q000378:metabolism*; D009154:Mutation; D011309:Preservation, Biological; D051381:Rats; D011919:Rats, Inbred Strains
D009153:Mutagens; D015127:9,10-Dimethyl-1,2-benzanthracene; D004128:Dimethylnitrosamine