Mutational specificity of benzo[a]pyrene diolepoxide in monkey cells.
Benzo[a]pyrene diolepoxide (BPDE) is thought to be the major mutagenic and carcinogenic intermediate in benzo[a]pyrene metabolism in mammalian cells. In order to test the mutagenic specificity of this compound in mammalian cells, we have used the pZ189 shuttle vector system to identify and analyze point mutations induced when DNA treated in vitro with BPDE is replicated in monkey cells. We find that point mutations occur almost exclusively at G.C base pairs; G.C----T.A and G.C----C.G transversions and single base pair deletions occur most frequently. This pattern is consistent with the known preferential covalent binding of BPDE to G residues.
D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide / Q000494:pharmacology*; D000818:Animals; D001482:Base Composition / Q000187:drug effects; D001483:Base Sequence; D002460:Cell Line; D002522:Chlorocebus aethiops; D004249:DNA Damage; D004101:Dihydroxydihydrobenzopyrenes / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects*; D005822:Genetic Vectors / Q000187:drug effects; D006147:Guanine; D007668:Kidney; D008969:Molecular Sequence Data; D009154:Mutation
D004101:Dihydroxydihydrobenzopyrenes; D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide; D006147:Guanine