High-resolution solid-state 13C nuclear magnetic resonance of bacterial spores: identification of the alpha-carbon signal of dipicolinic acid.
Natural-abundance solid-state 13C nuclear magnetic resonance spectra were obtained for bacterial spores for the first time by using the technique of cross-polarization magic-angle-spinning nuclear magnetic resonance spectroscopy. A resonance at about 150 ppm, detectable in spore samples having a Mn content of less than 0.05%, was consistent with an identification as the alpha-carbon signal of calcium dipicolinate; this signal was missing from a spore sample treated with acid to release dipicolinate and from a spore coat preparation. Carbohydrate peaks were particularly intense in spores and coat preparations of Bacillus macerans. Signals ascribable to beta-hydroxybutyrate were prominent in a B. cereus sample.
D001407:Bacillus / Q000378:metabolism*; D001409:Bacillus cereus / Q000378:metabolism; D001410:Bacillus megaterium / Q000378:metabolism; D001412:Bacillus subtilis / Q000378:metabolism; D002247:Carbon Isotopes; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D010848:Picolinic Acids / Q000378:metabolism*; D013045:Species Specificity; D013171:Spores, Bacterial / Q000378:metabolism
D002247:Carbon Isotopes; D010848:Picolinic Acids; C004860:dipicolinic acid