Biochemical and in vivo properties of commercial virus-inactivated factor VIII concentrates.
The following commercial virus-inactivated factor VIII concentrates were studied in vitro: AHF-Kabi and Octonativ, KabiVitrum; Hemofil T, Hyland; Factorate HP and Monoclate, Armour; Nordiocto, Nordisk Gentofte (dry heated); Kryobulin TIM3, Immuno (steam treated); Profilate, Alpha (heated as dry material slammed in heptane); Hemate P, Behring (wet heated) and Octa-V.I., Octapharma (solvent/detergent treated). The concentration of VIII:C was lowest in AHF-Kabi, whereas it ranged from 24 to 53 IU/ml in the high purity concentrates, except for Monoclate in which it ranged from 91-128 IU/ml. All concentrates but Octa-V.I. had higher values for VIII:Ag than for VIII:C. von Willebrand factor with normal distribution of multimers could only be demonstrated in AHF-Kabi and Hemate P. In vivo studies were performed in 12 severe hemophiliacs. Recovery and half-life of VIII:C did not differ between the various concentrates. Hemate P was given to 5 patients with severe von Willebrand's disease, in all of whom a correction of the hemostatic defect was seen.
D000941:Antigens / Q000302:isolation & purification; D003450:Cryoglobulins / Q000008:administration & dosage / Q000032:analysis; D005169:Factor VIII / Q000008:administration & dosage / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification; D006207:Half-Life; D006467:Hemophilia A / Q000097:blood / Q000628:therapy*; D006358:Hot Temperature; D006801:Humans; D008297:Male; D013227:Steam; D013242:Sterilization; D014775:Virus Activation / Q000187:drug effects; D014841:von Willebrand Factor / Q000008:administration & dosage / Q000032:analysis
D000941:Antigens; D003450:Cryoglobulins; D013227:Steam; D014841:von Willebrand Factor; D005169:Factor VIII