Sensitivity of different phases of the cell cycle to selected hepatotoxins in cultured liver-derived (BL9L) cells.
1. Many toxins are active against dividing cells and cytofluorometric analysis of synchronized dividing liver-derived (BL9L) cells has been employed to study the relative sensitivity of the G1(G0), S and G2/M phases of the cell cycle to selected hepatotoxins. 2. The cytotoxic metal beryllium, which inhibits cell division, caused a specific block at the G1 phase of the cell cycle. 3. Dehydroretronecine, an antimitotic metabolite of the hepatotoxic plant pyrrolizidine alkaloids, retarded progression of cells through the cell cycle with a consistent accumulation at the late S to G2 phase. 4. Exposure of cells to aflatoxin B1-8,9-epoxide, the putative carcinogenic metabolite of the hepatocarcinogen aflatoxin B1, particularly during the early period of S phase, produced morphologically transformed cells.
D016604:Aflatoxin B1; D000348:Aflatoxins / Q000633:toxicity; D000818:Animals; D002273:Carcinogens / Q000633:toxicity; D002453:Cell Cycle / Q000187:drug effects*; D002460:Cell Line; D004353:Drug Evaluation, Preclinical / Q000379:methods; D004847:Epithelial Cells; D004848:Epithelium / Q000187:drug effects; D005434:Flow Cytometry / Q000379:methods; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000473:pathology*; D016686:Monocrotaline / Q000031:analogs & derivatives*; D011763:Pyrrolizidine Alkaloids / Q000633:toxicity; D014118:Toxins, Biological / Q000494:pharmacology
D000348:Aflatoxins; D002273:Carcinogens; D011763:Pyrrolizidine Alkaloids; D014118:Toxins, Biological; D016686:Monocrotaline; D016604:Aflatoxin B1; C007241:dehydroretronecine