title: Inhibition of galactosyltransferase activity by cacodylate buffer.
abstract: Cacodylate buffer, frequently used in the assay of galactosyltransferase, causes inhibition of enzyme purified from human pleural effusion. The inhibition is reflected by an increase in the Km (UDP-galactose) and a decrease in Vmax and cannot be reversed by the addition of 2-mercaptoethanol. It is suggested that alternative buffers be used for the study of the enzyme.
mesh_terms: D001152:Arsenicals / Q000494:pharmacology*; D002021:Buffers; D002101:Cacodylic Acid / Q000494:pharmacology*; D005700:Galactosyltransferases / Q000037:antagonists & inhibitors*; D006801:Humans; D010996:Pleural Effusion / Q000201:enzymology
chemical_list: D001152:Arsenicals; D002021:Buffers; D002101:Cacodylic Acid; D005700:Galactosyltransferases