Analysis of polyamines as their dabsyl derivatives by reversed-phase high-performance liquid chromatography.
The polyamines putrescine, cadaverine, spermidine, and spermine and the corresponding mono-N-acetylpolyamines can be separated as their dimethylaminoazobenzenesulfonyl derivatives in a single analysis in less than 22 min. The method employs reversed-phase high-performance liquid chromatography (Spherisorb S5 ODS2 column) with an acetonitrile/acetate buffer gradient elution system and detection in the visible (436 nm) region. The detection limit for a single dimethylaminoazobenzenesulfonylpolyamine is less than 2 pmol.
D002103:Cadaverine / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D006863:Hydrogen-Ion Concentration; D011073:Polyamines / Q000032:analysis*; D011700:Putrescine / Q000032:analysis; D012486:Salmonella typhimurium / Q000032:analysis; D013095:Spermidine / Q000032:analysis; D013096:Spermine / Q000032:analysis; D004124:p-Dimethylaminoazobenzene / Q000031:analogs & derivatives*
D011073:Polyamines; D013096:Spermine; C010572:4-N,N-dimethylaminoazobenzene-4'-sulfonyl chloride; D004124:p-Dimethylaminoazobenzene; D002103:Cadaverine; D013095:Spermidine; D011700:Putrescine