Adipocyte plasma membranes contain two Gi subtypes but are devoid of Go.
Antisera generated against synthetic peptides were used to identify G-protein alpha-subunits in plasma membranes from rat adipocytes. Applying the immunoblot technique, we detected two Gs alpha-subunits of 42 and 43 kDa, corresponding to the two cholera toxin substrates, and two Gi alpha-subunits of 40 and 41 kDa, corresponding to the two pertussis toxin substrates present in these membranes. The 40 kDa protein was tentatively identified as the Gi2 alpha-subunit. A serum specific for the Go alpha-subunit failed to detect any immunoreactive protein. Thus plasma membranes of adipocytes possess two forms of Gi but not Go.
D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D000936:Antigen-Antibody Complex / Q000032:analysis; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D002772:Cholera Toxin / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000302:isolation & purification* / Q000378:metabolism; D007106:Immune Sera; D015151:Immunoblotting; D008297:Male; D051379:Mice; D008970:Molecular Weight; D051381:Rats; D011919:Rats, Inbred Strains
D000936:Antigen-Antibody Complex; D007106:Immune Sera; D002772:Cholera Toxin; D019204:GTP-Binding Proteins