[Mapping of apolipoprotein A-1 and ceruloplasmin genes on human, rat and mouse chromosomes by hybridization in situ with specific human DNA probes].
In situ hybridization was carried out on metaphase-prometaphase chromosomes of PGA-stimulated lymphocytes and bone marrow cells obtained from laboratory rats and mice. Plasmid cloned sequences of human apolipoprotein A-1 (Apo A-1) and ceruloplasmin (CP) cDNA fragments have been used as specific probes labelled in nick-translation reaction with 3HdTTP and 3Hd ATP. The data of our study suggest that Apo A-1 is localized in 11q14-22, 9 A2-4 and 5q36 areas in men, mice and rats, respectively. The DNA sequences of human CP cDNA most probably occupy 3q23-25, 13q24-26 and 15q13-20 areas. Heterologous in situ hybridization of other species with DNA probes does not always give reliable results in gene mapping. Thus, the data of heterologous hybridization should be considered with caution.
D000818:Animals; D016632:Apolipoprotein A-I; D001054:Apolipoproteins A / Q000235:genetics*; D002570:Ceruloplasmin / Q000235:genetics*; D002874:Chromosome Mapping; D002875:Chromosomes / Q000032:analysis; D002880:Chromosomes, Human, Pair 11 / Q000032:analysis; D004247:DNA; D006801:Humans; D051379:Mice; D009693:Nucleic Acid Hybridization; D051381:Rats; D013045:Species Specificity
D016632:Apolipoprotein A-I; D001054:Apolipoproteins A; D004247:DNA; D002570:Ceruloplasmin
Kartirovanie genov apolipoproteina A-1 i tseruloplazmina na khromosomakh cheloveka, krys i mysheĭ metodom gibridizatsii in situ so spetsificheskimi DNK-zondami cheloveka.