A 28-bp segment of the Saccharomyces cerevisiae PHO5 upstream activator sequence confers phosphate control to the CYC1-lacZ gene fusion.
Two regions within the Saccharomyces cerevisiae PHO5 upstream activator sequence (UAS) are involved in phosphate dependent transcription activation [Rudolph and Hinnen, Proc. Natl. Acad. Sci. USA 84 (1987) 1340-1344]. In experiments carried out in vivo we showed that one of these can compensate for the CYC1 UAS and expresses the heterologous CYC1-lacZ gene in response to phosphate starvation. A 28-bp segment is very efficient in gene activation, and a 19-bp subsegment that corresponds to the UASp consensus sequence brings about a weak but still detectable activation. As was observed with other UAS, gene activation is obtained with either orientation of the element, and tandem copies yield double lacZ activity compared to a single copy. No gene activation is observed in a pho4 and in a pho2 mutant. Absence of PHO2 reduces the basal expression of CYC1.
D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D007763:Lac Operon; D009154:Mutation; D010710:Phosphates; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics; D014158:Transcription, Genetic; D001616:beta-Galactosidase / Q000032:analysis
D010710:Phosphates; D001616:beta-Galactosidase