Murine osteoblastlike cells and the osteogenic cell MC3T3-E1 release a macrophage colony-stimulating activity in culture.
The osteoclast may be of hematopoietic lineage and as such its development could be regulated by colony-stimulating factors. Since there is much interest as to whether osteoblasts influence bone resorption, we examined whether bone cells produce colony-stimulating activity. Both cells isolated from neonatal calvaria and the osteogenic cell MC3T3-E1 were found to constitutively release a colony-stimulating activity possessing characteristics of a macrophage colony-stimulating factor, as determined by basic biochemical purification and by identity of colonies induced in cultures of bone marrow cells. Release could be increased by the presence of the bone-resorbing agents lipopolysaccharide and 1,25 dihydroxyvitamin D3. We conclude that the osteoblast may contribute to both the processes of osteoclast formation and of hematopoiesis through the secretion of colony-stimulating activity into the adjacent bone marrow.
D000818:Animals; D002117:Calcitriol / Q000494:pharmacology; D002460:Cell Line; D002478:Cells, Cultured; D002850:Chromatography, Gel; D003115:Colony-Stimulating Factors / Q000096:biosynthesis*; D006410:Hematopoiesis; D066298:In Vitro Techniques; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000502:physiology*; D051379:Mice; D010006:Osteoblasts / Q000378:metabolism*; D012886:Skull / Q000378:metabolism
D003115:Colony-Stimulating Factors; D008070:Lipopolysaccharides; D002117:Calcitriol