Anti-CD3 activation of T cells with immunoglobulin coupled red cells.
We have studied the ability of anti-mouse IgG antibody coupled to Burro red blood cells (BRBC) to modulate the anti-CD3 stimulation of human T cells. These coupled BRBC restored anti-Leu-4 (anti-CD3) induced proliferative responses to purified T cells, in the presence of interleukin-1 (IL-1). Uncoupled BRBC were incapable of supporting anti-Leu-4-induced stimulation. T cells from individuals whose peripheral blood mononuclear cells were unresponsive to anti-Leu-4 did proliferative in the presence of anti-Leu-4, IL-1, and coupled BRBC. Ig-coupled red cells provide an efficient method for the study of murine antibody-induced modulation of T cell surface antigens.
D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface / Q000276:immunology*; D004912:Erythrocytes / Q000276:immunology*; D006801:Humans; D007136:Immunoglobulins / Q000276:immunology*; D008213:Lymphocyte Activation; D051379:Mice; D013601:T-Lymphocytes / Q000276:immunology*
D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface; D007136:Immunoglobulins