Preparation by ultrafiltration and control by high-performance liquid chromatography of the native hapten of Brucella abortus for use in radial immunodiffusion diagnostic test.
An effective method was developed for the preparation of Brucella abortus native hapten by use of an ultrafiltration system. The membranes PM30 and YM5 were used to separate the lipopolysaccharide and the native hapten. The yield of the native hapten was higher than that obtained by a more complex procedure reported previously. This method is economical for the large-scale preparation of B. abortus native hapten. The effects of ultrafiltration were evaluated by a quick and sensitive high-performance liquid chromatographic technique.
D000818:Animals; D000942:Antigens, Bacterial / Q000302:isolation & purification; D002003:Brucella abortus / Q000276:immunology*; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D006241:Haptens / Q000302:isolation & purification*; D006868:Hydrolysis; D005779:Immunodiffusion; D014462:Ultrafiltration
D000942:Antigens, Bacterial; D006241:Haptens