Rapid expression of c-fos specific messenger RNA after wounding of a BALB/c-3T3 fibroblast monolayer.
A grid of damaged areas was introduced into serum-starved BALB/c-3T3 fibroblast monolayers. The expression of c-fos specific messenger RNA was examined by the use of the cytoplasmic dot hybridisation technique 15, 30 and 45 min after wounding. The expression of c-fos messenger RNA was highest after 30 min, while microglobulin was constantly expressed. These results show that the unspecific event of wounding induces the specific expression of c-fos messenger RNA, an effect similar to that of growth factors during serum stimulation.
D000818:Animals; D002478:Cells, Cultured; D005347:Fibroblasts / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009693:Nucleic Acid Hybridization; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis*; D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger / Q000302:isolation & purification / Q000378:metabolism*
D011518:Proto-Oncogene Proteins; D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger