Genetic restriction of the murine humoral response to a recombinant Plasmodium vivax circumsporozoite protein.
The antibody response of inbred and congenic strains of mice immunized with a recombinant Plasmodium vivax circumsporozoite protein was investigated. Only mice of the H-2k and H-2a haplotypes developed antibodies that react with P. vivax sporozoite in an indirect immunofluorescence assay and with the recombinant protein in enzyme-linked immunosorbent assay. Congenic recombinant strains were used to map Ir gene control to the I-Ak locus. A similar pattern of genetic restriction was found when congenic strains were immunized with a carrier-free synthetic peptide corresponding to the repeat region of the P. vivax circumsporozoite protein. The results suggest that the ability of T helper cells to recognize a T cell epitope(s) in the repeat region of the P. vivax circumsporozoite protein is genetically restricted.
D000818:Animals; D000917:Antibody Formation; D000953:Antigens, Protozoan / Q000276:immunology*; D000954:Antigens, Surface / Q000378:metabolism*; D005802:Genes, MHC Class II; D000949:Histocompatibility Antigens Class II; D051379:Mice; D009842:Oligopeptides / Q000276:immunology; D010966:Plasmodium vivax / Q000276:immunology*; D015800:Protozoan Proteins; D011994:Recombinant Proteins / Q000276:immunology
D000953:Antigens, Protozoan; D000954:Antigens, Surface; D000949:Histocompatibility Antigens Class II; D009842:Oligopeptides; D015800:Protozoan Proteins; D011994:Recombinant Proteins; C041595:circumsporozoite protein, Protozoan