title: Comparison of indium 111 oxine-labeled platelet aggregation between sutured and argon laser-assisted vascular anastomoses.
abstract: The thrombogenicity of argon laser-assisted vascular anastomoses (LAVAs) was compared with that of sutured vascular anastomoses (SVAs) by measurement of platelet aggregation at the site of repair in a canine model. Sequential 1 cm longitudinal carotid and femoral arteriotomies (n = 80) or jugular and femoral phlebotomies (n = 80) were performed, with each vessel having two tandem, randomly positioned arteriotomies or phlebotomies separated by a 4 cm length of intact vessel. One incision was repaired by SVA with continuous 6-0 polypropylene sutures and the other by argon LAVA. For the laser fusions, argon laser energy was applied to the adventitial surface of the vessel with a 300 micron fiberoptic probe with 0.5 W power, 1100 joules per square centimeter energy fluence, and 150 second exposure per 1 cm length. The arterial and venous segments of SVAs and LAVAs and an equivalent length of normal vessel were harvested at 48 hours (n = 16, 16, 16), 2 weeks (n = 12, 12, 12), and 4 weeks (n = 12, 12, 12). Autologous indium 111 oxine-labeled platelets were injected intravenously 48 hours before removal of the vascular repairs and the radioactivity of the specimens was determined on removal with a NaI (T1) well-type scintillation counter. Anastomotic platelet adherence index (APAI) was calculated as the ratio of emissions of SVA or LAVA to normal reference vessel.(ABSTRACT TRUNCATED AT 250 WORDS)
mesh_terms: D000714:Anastomosis, Surgical; D000818:Animals; D002339:Carotid Arteries / Q000000981:diagnostic imaging / Q000601:surgery; D004285:Dogs; D005069:Evaluation Studies as Topic; D005263:Femoral Artery / Q000000981:diagnostic imaging / Q000601:surgery; D005268:Femoral Vein / Q000000981:diagnostic imaging / Q000601:surgery; D006912:Hydroxyquinolines; D007205:Indium Radioisotopes; D007601:Jugular Veins / Q000000981:diagnostic imaging / Q000601:surgery; D053685:Laser Therapy / Q000009:adverse effects* / Q000379:methods; D008954:Models, Biological; D009942:Organometallic Compounds; D015125:Oxyquinoline / Q000031:analogs & derivatives; D010974:Platelet Aggregation; D011877:Radionuclide Imaging; D011897:Random Allocation; D013536:Suture Techniques; D013997:Time Factors; D014654:Vascular Patency; D014656:Vascular Surgical Procedures
chemical_list: D006912:Hydroxyquinolines; D007205:Indium Radioisotopes; D009942:Organometallic Compounds; C032950:indium oxine; D015125:Oxyquinoline