Hydroxyprolyl3-bradykinin in high molecular mass kininogen. Presence in human and monkey kininogens, but not in kininogens from bovine, rat, rabbit, guinea pig and mouse plasmas.
The contents of hydroxyprolyl3-bradykinin in high molecular mass (HMM) kininogens from human and animal plasmas were examined by reversed-phase HPLC following their proteolytic scission by bovine plasma kallikrein. The relative contents of hydroxyprolyl3-bradykinin in kinins from HMM kininogens from pooled plasmas of human and monkey origin were 33 and 73%, respectively. On the other hand, hydroxyprolyl3-bradykinin could not be detected in HMM kininogen preparations from bovine, rat, guinea pig, rabbit and mouse plasmas. Hydroxyproline in hydroxyprolyl3-bradykinin was assigned as trans-4-hydroxyproline by comparison of the retention times on reversed-phase HPLC with isomers of hydroxyproline after derivatization with 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole chloride.
D000595:Amino Acid Sequence; D000818:Animals; D001920:Bradykinin / Q000031:analogs & derivatives* / Q000097:blood / Q000302:isolation & purification; D002417:Cattle; D006168:Guinea Pigs; D000882:Haplorhini; D006801:Humans; D007704:Kininogens / Q000097:blood* / Q000302:isolation & purification; D051379:Mice; D011817:Rabbits; D051381:Rats; D012016:Reference Values; D013045:Species Specificity
D007704:Kininogens; C021698:bradykinin, hydroxy-Pro(3)-; D001920:Bradykinin