Transport of leucine, lysine, glycine and aspartate in neuroblastoma C1300 and glioma C6 cells.
Non-saturable penetration and the V and Km constants of saturable influx of leucine, lysine and glycine were always greater in cultured neuroblastoma (C1300) than in glioma (C6) cells. Aspartate uptake was detected only in glioma cells. Unstimulated efflux of the amino acids was initially fast in both cell types but soon slowed down. The efflux of glycine and aspartate exhibited no heteroexchange, the efflux of lysine was stimulated by extracellular leucine and that of leucine slightly by lysine and glycine but only in glioma cells.
D000818:Animals; D001224:Aspartic Acid / Q000493:pharmacokinetics*; D002461:Cell Line, Transformed; D005910:Glioma / Q000378:metabolism*; D005998:Glycine / Q000493:pharmacokinetics*; D006801:Humans; D007930:Leucine / Q000493:pharmacokinetics*; D008239:Lysine / Q000493:pharmacokinetics*; D009447:Neuroblastoma / Q000378:metabolism*
D001224:Aspartic Acid; D007930:Leucine; D008239:Lysine; D005998:Glycine