Thyroid releasing hormone inhibits testosterone metabolism by rat prostate in vitro.
A method involving thin layer chromatography (TLC) was developed to assess 5 alpha-DHT-reductase activity in rat ventral prostate, in terms of the amount of testosterone metabolised to its main 5 alpha-reduced compound, dihydrotestosterone (DHT). Using this method an in vitro study was carried out to determine 5 alpha-DHT reductase activity in the rat ventral prostate as modulated by thyroid releasing hormone (TRH). The study revealed a decrease in the enzyme activity with increasing doses of TRH, as shown by a decrease in the extent of reduction of testosterone to DHT. The ratios of the counts for DHT and testosterone however did not show any dose related effect.
D058891:5-alpha Reductase Inhibitors; D000818:Animals; D002855:Chromatography, Thin Layer; D013196:Dihydrotestosterone / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D008297:Male; D011467:Prostate / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D051381:Rats; D013739:Testosterone / Q000037:antagonists & inhibitors* / Q000378:metabolism; D013973:Thyrotropin-Releasing Hormone / Q000494:pharmacology*
D058891:5-alpha Reductase Inhibitors; D013196:Dihydrotestosterone; D013739:Testosterone; D013973:Thyrotropin-Releasing Hormone