Methylcellulose inhibition of exo-beta-1,4-glucanase A from Ruminococcus flavefaciens FD-1.
A homogeneous preparation of exo-beta-1,4-glucanase A from Ruminococcus flavefaciens FD-1 was competitively inhibited by low concentrations (less than 3 mM) of methylcellulose. The enzyme was also sensitive to the surfactant properties of methylcellulose at high methylcellulose concentrations.
D000818:Animals; D044643:Glucan 1,4-beta-Glucosidase; D005959:Glucosidases / Q000037:antagonists & inhibitors*; D006868:Hydrolysis; D007700:Kinetics; D008747:Methylcellulose / Q000378:metabolism / Q000494:pharmacology*; D010459:Peptococcaceae / Q000201:enzymology*; D013501:Surface-Active Agents / Q000494:pharmacology; D001617:beta-Glucosidase / Q000037:antagonists & inhibitors*
D013501:Surface-Active Agents; D008747:Methylcellulose; D005959:Glucosidases; D001617:beta-Glucosidase; D044643:Glucan 1,4-beta-Glucosidase