Preparation and regeneration of bacteriophage PL-1 enzyme-induced Lactobacillus casei protoplasts.
Lactobacillus casei ATCC 27092 protoplasts were obtained by treatment with a bacteriolytic enzyme, which was produced in the PL-1 phage lysates of this bacterium, at 37 degrees C for 1.5 h in 50 mM Tris hydrochloride buffer (pH 7.2) containing 20% sucrose and 10 mM MgSO4. The protoplasts lacked the cell wall layer but retained the ability to transport L-[3H]glutamine. The frequency of regeneration was about 1%, whereas about 99% of the cells were osmotically sensitive.
D001433:Bacteriolysis; D001435:Bacteriophages / Q000201:enzymology*; D001693:Biological Transport, Active; D002473:Cell Wall / Q000648:ultrastructure; D005973:Glutamine / Q000378:metabolism; D007780:Lacticaseibacillus casei / Q000378:metabolism / Q000648:ultrastructure*; D008854:Microscopy, Electron; D009996:Osmotic Fragility; D011523:Protoplasts / Q000378:metabolism / Q000648:ultrastructure*
D005973:Glutamine