title: Structure and regulation of the anthranilate synthase genes in Pseudomonas aeruginosa: II. Cloning and expression in Escherichia coli.
abstract: The genes for the large and small subunits of anthranilate synthase (trpE and trpG, respectively) have been cloned from Pseudomonas aeruginosa PAC174 into E. coli by R-prime formation with the broad-host-range plasmid R68.44. Sequential subcloning into plasmid vectors reduced the active Pseudomonas DNA fragment to a length of 3.1 kb. We obtained evidence that this region contains the promoter for its own expression and retains a vestigial regulatory response to tryptophan scarcity or excess.
mesh_terms: D000878:Anthranilate Synthase / Q000235:genetics*; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli; D005786:Gene Expression Regulation; D005796:Genes; D005798:Genes, Bacterial; D011550:Pseudomonas aeruginosa / Q000235:genetics*; D014364:Tryptophan / Q000502:physiology*
chemical_list: D004269:DNA, Bacterial; D014364:Tryptophan; D000878:Anthranilate Synthase