Antigen-specific augmentation factor involved in murine delayed-type footpad reaction. III. Genetic restriction of delayed hypersensitivity augmentation factor (DAF).
We found an antigen-specific factor capable of augmenting delayed-type hypersensitivity (DTH) in the culture supernatant of the mixture of immune T cells and specific antigen, or in the serum of mice immunized with xenogeneic erythrocytes and elicited for DTH footpad reaction. Previous experiments on the genetic restriction of this factor (DTH-augmentation factor; DAF) indicated that DAF activity was effective across the MHC-barrier in C3H/He (H-2k)--BALB/c (H-2d) system. The genetic restriction between DAF and its acceptor cells was then investigated precisely using Igh (immunoglobulin heavy chain locus)-congeneic mice: 1) Expression of DAF activity was MHC-nonrestricted, 2) but was restricted by the Igh-linked gene on the 12th chromosome, 3) such Igh-linked gene restriction was also demonstrated by an absorption test with normal spleen cells. The acceptor cells for DAF were Thy-1+,L3T4+,Lyt-2- T cells.
D000818:Animals; D000941:Antigens / Q000276:immunology; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology; D000950:Antigens, Ly / Q000276:immunology; D005260:Female; D005803:Genes, Immunoglobulin; D005802:Genes, MHC Class II; D006968:Hypersensitivity, Delayed; D008222:Lymphokines / Q000235:genetics* / Q000276:immunology; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D013045:Species Specificity; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology
D000941:Antigens; D000945:Antigens, Differentiation, T-Lymphocyte; D000950:Antigens, Ly; D008222:Lymphokines