Reduction of yellow fever virus mouse neurovirulence by immunization with a bacterially synthesized non-structural protein (NS1) fragment.
Part of a yellow fever virus-specified non-structural protein (NS1) was expressed in Escherichia coli as a fusion protein with beta-galactosidase. Immunization of mice with this partially purified NS1-beta-galactosidase fusion protein induced yellow fever virus-specific antibodies and provided some protection against intracerebral challenge with the virus.
D000818:Animals; D001921:Brain / Q000382:microbiology; D003001:Cloning, Molecular; D003168:Complement Fixation Tests; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D005260:Female; D005455:Fluorescent Antibody Technique; D007114:Immunization; D051379:Mice; D011993:Recombinant Fusion Proteins / Q000276:immunology; D014614:Vaccines, Synthetic; D014709:Vero Cells; D014764:Viral Proteins / Q000276:immunology*; D014765:Viral Vaccines; D014774:Virulence; D015004:Yellow Fever / Q000517:prevention & control*; D015005:Yellow fever virus / Q000276:immunology* / Q000472:pathogenicity; D001616:beta-Galactosidase / Q000276:immunology
D011993:Recombinant Fusion Proteins; D014614:Vaccines, Synthetic; D014764:Viral Proteins; D014765:Viral Vaccines; D001616:beta-Galactosidase