C-fos gene activation in murine thymocytes by a mechanism independent of protein kinase C or a Ca2+ signal.
The accumulation of c-fos mRNA in mouse thymocytes was compared when the cells were stimulated by concanavalin A (Con A), the Ca2+ ionophore A23187 or the phorbol ester, TPA, either separately or by combinations of these mitogens. The c-fos response to mitogenic concentrations of Con A could not be attributed either to the rise in [Ca2+]i it induces or to activation of protein kinase C. Thus, although Con A causes the breakdown of phosphatidylinositol 4,5-bisphosphate in these cells, neither of the signals which can be generated by this response was responsible for the activation of the c-fos gene by Con A. This implies that some other unidentified signal generated by Con A activates the c-fos gene.
D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D003208:Concanavalin A / Q000494:pharmacology; D004347:Drug Interactions; D004789:Enzyme Activation; D005786:Gene Expression Regulation / Q000187:drug effects*; D051379:Mice; D008938:Mitosis; D011493:Protein Kinase C / Q000378:metabolism*; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D016760:Proto-Oncogene Proteins c-fos; D013601:T-Lymphocytes / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D015533:Transcriptional Activation
D011518:Proto-Oncogene Proteins; D016760:Proto-Oncogene Proteins c-fos; D003208:Concanavalin A; D000001:Calcimycin; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium