Strategy for structural characterization of haemoglobin variants.
High-performance liquid chromatography allows one to perform effective and rapid separations on microquantities of material. The miniaturization of the techniques led to new approaches involving isoelectric focusing (IEF) of immobilized pH gradient IEF for haemoglobin preparation, successive enzymic hydrolyses for isolating abnormal peptides and amino acid composition determination after precolumn derivatization.
D000596:Amino Acids / Q000032:analysis; D014644:Genetic Variation; D006454:Hemoglobins / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D010446:Peptide Fragments / Q000032:analysis / Q000302:isolation & purification; D010449:Peptide Mapping
D000596:Amino Acids; D006454:Hemoglobins; D010446:Peptide Fragments