Biomonitoring of oil spill in a boreal archipelago by xenobiotic biotransformation in perch (Perca fluviatilis).
The effect of the accidental oil spill (250 tons) in a boreal archipelago (Gulf of Bothnia, Vaasa, Finland) on xenobiotic metabolism of local perch (Perca fluviatilis) was monitored for 1.5 years. The monooxygenase (benzo[a]pyrene, 7-ethoxycoumarin O-deethylase, 7-ethoxyresorufin O-deethylase) and conjugation (UDPglucuronosyltransferase, glutathione S-transferase) activities of perch liver were determined from control areas and those areas where oil had spilled. Only a slight induction in monooxygenase activities was seen in perch caught near the oil spill 4 months after the accident. The induction of monooxygenase activities detected with the fuel oil in laboratory experiments was, however, clear. After a single dose, it rose rapidly and quickly disappeared. Conjugation enzyme activities were not affected in the laboratory.
D015660:7-Alkoxycoumarin O-Dealkylase; D000059:Accidents; D000818:Animals; D001579:Benzopyrene Hydroxylase / Q000032:analysis; D001711:Biotransformation; D019363:Cytochrome P-450 CYP1A1; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D005387:Finland; D005648:Fuel Oils / Q000633:toxicity*; D014453:Glucuronosyltransferase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D009251:NADPH-Ferrihemoprotein Reductase / Q000378:metabolism; D009929:Organ Size; D010088:Oxidoreductases / Q000378:metabolism; D010105:Oxygenases / Q000378:metabolism; D010471:Perches / Q000378:metabolism*; D010473:Perciformes / Q000378:metabolism*; D010578:Petroleum / Q000633:toxicity*; D012621:Seasons; D013696:Temperature; D013997:Time Factors; D014873:Water Pollutants / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D005648:Fuel Oils; D010578:Petroleum; D014873:Water Pollutants; D014874:Water Pollutants, Chemical; D003577:Cytochrome P-450 Enzyme System; D010088:Oxidoreductases; D010105:Oxygenases; D015660:7-Alkoxycoumarin O-Dealkylase; D001579:Benzopyrene Hydroxylase; D019363:Cytochrome P-450 CYP1A1; D009251:NADPH-Ferrihemoprotein Reductase; D014453:Glucuronosyltransferase; D005982:Glutathione Transferase