An inhibitor of the UDP-N-acetylgalactosamine:GM3, N-acetylgalactosaminyltransferase: purification and properties, and preparation of an antibody to this inhibitor.
An inhibitor of the UDP-N-acetylgalactosamine:GM3, N-acetylgalactosaminyltransferase (EC 2.4.1.92) has been purified close to 100-fold from chicken blood serum. The method of purification includes heating, dialysis, passage through a column of DEAE-Sephadex, filtration through Amicon XM 100, and passage through Sepharose 6B. The molecular weight determined by Sepharose 6B was 200,000, but on sodium dodecyl sulfate-polyacrylamide gel electrophoresis it appears as if the compound dissociated into components of 68,000. The inhibitor was not active on other glycosyl transferases and lost its inhibitory activity following treatment with pronase and trypsin. alpha-Chymotrypsin did not affect the inhibitor. An antibody to this inhibitor was prepared which decreased its inhibitory capability and precipitated with it in a radial double immunodiffusion experiment.
D000818:Animals; D002645:Chickens; D002852:Chromatography, Ion Exchange; D003956:Dialysis; D004591:Electrophoresis, Polyacrylamide Gel; D004791:Enzyme Inhibitors / Q000097:blood* / Q000276:immunology; D005374:Filtration; D005700:Galactosyltransferases / Q000037:antagonists & inhibitors*; D006358:Hot Temperature; D007106:Immune Sera / Q000494:pharmacology; D005779:Immunodiffusion; D008970:Molecular Weight; D017350:N-Acetylgalactosaminyltransferases; D011402:Pronase / Q000378:metabolism; D014357:Trypsin / Q000378:metabolism; D000097763:Polypeptide N-acetylgalactosaminyltransferase
D004791:Enzyme Inhibitors; D005700:Galactosyltransferases; D007106:Immune Sera; D017350:N-Acetylgalactosaminyltransferases; D011402:Pronase; D014357:Trypsin; D000097763:Polypeptide N-acetylgalactosaminyltransferase; C023220:(N-acetylneuraminyl)-galactosylglucosylceramide N-acetylgalactosaminyltransferase