Cytoplasmic membrane fluidity measurements on intact living cells of Bacillus subtilis by fluorescence anisotropy of 1,6-diphenyl-1,3,5-hexatriene.
A method for determination of membrane fluidity (microviscosity) in Bacillus subtilis cytoplasmic membrane under in vivo conditions is described. The membranes were labelled with the hydrophobic fluorescent probe 1,6-diphenyl-1,3,5-hexatriene during the exponential phase of growth. Fluorescence anisotropy measurements were carried out in an intact cell suspension having absorbance A as high as 0.2-0.3 (corresponding to a cell concentration of 100-300/nL).
D001412:Bacillus subtilis / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D004161:Diphenylhexatriene; D005454:Fluorescence Polarization; D007700:Kinetics; D008560:Membrane Fluidity; D011523:Protoplasts / Q000378:metabolism; D013816:Thermodynamics
D004161:Diphenylhexatriene