Dynamic phosphorus-31 NMR study of sugar cataract and its prevention in rat lenses with aldose reductase inhibitors.
P31 NMR spectroscopy was used to study dynamic changes in rat lens phosphate metabolites under hyperglycemic conditions. NMR control spectra were collected at 37 degrees C with normal media; NMR spectra were also collected under the same conditions except the normal media was substituted for one containing 30 mM xylose. There was a significant increase in intensity of the sugar phosphate NMR signal as well as a moderate decrease in intensity for the three ATP peaks. In separate experiments normal rat lenses were pre-treated with perfusion media containing the ARIs; either Spiro-(2-fluorofluorene-9,4'-imidazolidine)-2',5'-dione (AL theta 1567) or Spiro-(2,7-difluorofluorene-9,4'-imidazolidine)-2',5'-dione (AL theta 1576) for three hours before changing to a xylose plus ARI media. After overnight perfusion, no appreciable change in the rat lens NMR spectra was found. This NMR observation indicates that lenses can be protected from hyperglycemic stress in the presence of the ARIs AL theta 1567 or AL theta 1576.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000449:Aldehyde Reductase / Q000037:antagonists & inhibitors*; D000818:Animals; D002386:Cataract / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*; D046508:Culture Techniques; D005449:Fluorenes / Q000494:pharmacology; D006827:Hydantoins / Q000494:pharmacology; D007908:Lens, Crystalline / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D010710:Phosphates / Q000378:metabolism; D010767:Phosphorylcholine / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013401:Sugar Alcohol Dehydrogenases / Q000037:antagonists & inhibitors*; D013997:Time Factors; D014994:Xylose
D005449:Fluorenes; D006827:Hydantoins; D010710:Phosphates; D010767:Phosphorylcholine; C045887:alconil; D000255:Adenosine Triphosphate; D014994:Xylose; D013401:Sugar Alcohol Dehydrogenases; D000449:Aldehyde Reductase