Immuno-light and immuno-electron microscopic examination of freeze-dried kidney tissue.
Correlative morphology of kidney tissue can be accomplished by a combination of immunofluorescent staining and light microscopy using a pre-embedding technique. Further development of this method has been elaborated by freeze-drying and chopping of the tissue, which then is incubated in biotinylated immunoglobulins. Fluorochromes and colloidal gold can be coupled to the biotinylated immunoglobulins in a second step. This technique is an alternative to postembedding immunogold staining for immunoelectron microscopy, and permits a correlative immunohistochemical and detailed morphological study of kidney tissue.
D000936:Antigen-Antibody Complex / Q000032:analysis; D001706:Biopsy; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes; D005612:Freeze Drying; D005921:Glomerulonephritis / Q000473:pathology*; D006801:Humans; D007668:Kidney / Q000166:cytology* / Q000473:pathology / Q000648:ultrastructure; D008854:Microscopy, Electron / Q000379:methods; D013861:Thiocyanates
D000936:Antigen-Antibody Complex; D005452:Fluoresceins; D005456:Fluorescent Dyes; D013861:Thiocyanates; D016650:Fluorescein-5-isothiocyanate