[Differential phosphorylation of the 3 forms of alkaline phosphatase of the small intestine of adult rats].
Semi-purified preparations were obtained from duodenal, jejunal or ileal mucosa containing one of the three alkaline phosphatase forms. The Mr of the isoenzymes were for F1 120, F2 150, F3 180 kDa. F1 was the only species found in the ileum; F2 was duodenal but mainly jejunal; F3 was found mainly in duodenum. These enzymes forms were the only phosphorylable proteins in these preparations. Following treatment with denaturing agents they were separated on gel electrophoresis into monomers F': F'1 65, F'2 65 and 90, F'3 90 kDa. Thus F2 could be an heterodimer. All were far more phosphorylated from ATP than from inorganic phosphate. As compared with F1, F3 was relatively more sensitive to ATP and less sensitive to inorganic phosphate.
D000469:Alkaline Phosphatase / Q000378:metabolism*; D000818:Animals; D004386:Duodenum / Q000201:enzymology; D007082:Ileum / Q000201:enzymology; D007413:Intestinal Mucosa / Q000201:enzymology; D007421:Intestine, Small / Q000201:enzymology*; D007583:Jejunum / Q000201:enzymology; D010766:Phosphorylation; D051381:Rats; D011919:Rats, Inbred Strains
D000469:Alkaline Phosphatase
Phosphorylation différentielle des trois formes de phosphatase alcaline de l'intestin grêle du rat adulte.