Identification of lysine residue 199 of human serum albumin as a binding site for benzylpenicilloyl groups.
Cyanogen bromide and tryptic fragments of penicilloylated serum albumin from penicillin-treated patients were separated by HPLC. Determinations of benzylpenicilloyl (BPO) were performed on the different fractions. A BPO containing peptide was identified by its amino acid sequence and the bound BPO group was located on the lysine residue 199.
D001665:Binding Sites; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D008239:Lysine; D010400:Penicillin G / Q000378:metabolism*; D011485:Protein Binding; D012709:Serum Albumin / Q000302:isolation & purification / Q000378:metabolism*
D012709:Serum Albumin; D008239:Lysine; D010400:Penicillin G