Cytoprotective and ulcer healing properties of prostaglandin E2, colloidal bismuth and sucralfate in rats.
This study describes the model of chronic gastric and duodenal ulcerations induced by the application of acetic acid on a strictly defined area of the serosal surface of the stomach and duodenum for 10 and 20 s, respectively. Acetic acid applied for longer (20-60 s) or on a larger area (28-64 mm2) resulted in the formation of severe ulcerations which penetrated into the surrounding organs and had very prolonged healing time. Ulcers induced by the application of acetic acid for 10-20 s on a smaller area (7-13.8 mm2) healed spontaneously within 2-3 weeks, thus constituting a model suitable for evaluation of drugs affecting the process of ulcer healing. Our preliminary results of 7- to 14-day treatment with certain drugs indicate that sucralfate and De-Nol, at the dose which does not affect gastric acid secretion, accelerated the healing rate of both gastric and duodenal ulcers so that the observed ulcer healing effect could be attributed to their ulcer healing property. In contrast, 16, 16-dimethyl PGE2 (dmPGE2) in cytoprotective dose was completely ineffective in enhancing ulcer healing. Higher, gastric inhibitory dose of dmPGE2 accelerated the healing of duodenal but not gastric ulcerations, indicating that the inhibition of gastric secretion rather that cytoprotective activity is responsible for ulcer healing effect of this prostaglandin.
D000818:Animals; D001729:Bismuth / Q000627:therapeutic use*; D002908:Chronic Disease; D015232:Dinoprostone; D004195:Disease Models, Animal; D005744:Gastric Acid / Q000378:metabolism; D005753:Gastric Mucosa / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D005755:Gastrins / Q000097:blood; D008297:Male; D010434:Pepsin A / Q000378:metabolism; D010437:Peptic Ulcer / Q000188:drug therapy*; D011458:Prostaglandins E / Q000627:therapeutic use*; D051381:Rats; D011919:Rats, Inbred Strains; D013392:Sucralfate / Q000627:therapeutic use*
D005755:Gastrins; D011458:Prostaglandins E; D013392:Sucralfate; D010434:Pepsin A; D015232:Dinoprostone; D001729:Bismuth