Ca2+-dependent NAD(P)+-induced alterations of rat liver and hepatoma mitochondrial membrane permeability.
Deenergized rat liver or AS-30D hepatoma mitochondria underwent extensive swelling when incubated in the presence of Ca2+ and an oxidant of mitochondrial pyridine nucleotides. This swelling was stimulated by phosphate and inhibited by ruthenium red, Mg2+ plus ATP and dibucaine. The degree of inhibition by dibucaine was shown to vary inversely with the Ca2+ concentration. The inhibition caused by ruthenium red was completely removed by the Ca2+ ionophore ionomycin indicating that the effect of the cation is mediated by internal binding sites. Since mitochondria were totally deenergized the data definitely rule out the requirement for Ca2+ cycling across the membrane in this mechanism.
D000818:Animals; D002122:Calcium Chloride / Q000494:pharmacology*; D003958:Diamide / Q000494:pharmacology; D003992:Dibucaine / Q000494:pharmacology; D004533:Egtazic Acid / Q000494:pharmacology; D004987:Ethers / Q000494:pharmacology; D007425:Intracellular Membranes / Q000187:drug effects / Q000378:metabolism*; D015759:Ionomycin; D007700:Kinetics; D008114:Liver Neoplasms, Experimental / Q000378:metabolism*; D008297:Male; D008928:Mitochondria / Q000378:metabolism*; D008930:Mitochondria, Liver / Q000378:metabolism*; D008933:Mitochondrial Swelling / Q000187:drug effects; D009243:NAD / Q000494:pharmacology*; D009249:NADP / Q000494:pharmacology*; D010539:Permeability; D051381:Rats; D011919:Rats, Inbred Strains
D004987:Ethers; D009243:NAD; D003958:Diamide; D004533:Egtazic Acid; D009249:NADP; D015759:Ionomycin; D003992:Dibucaine; D002122:Calcium Chloride