New 111In labeling of IgG: 111In-oxine mediated chelation.
Current methods of 111In chelate conjugation labeling of antibodies expose the protein to pH 5-6 during 111In chelation. These conditions could be detrimental if the antibody is acid labile. We have successfully labeled human IgG via the cyclic anhydride of DPTA and 111In-oxyquinoline(oxine). Chelation was achieved at pH 6.9-8.4 and was complete within 1 min at room temperature. The chelation was sensitive to trace metal contamination on labware and in some reagents (including commercial 111In-oxine).
D006801:Humans; D006912:Hydroxyquinolines; D007074:Immunoglobulin G; D007205:Indium Radioisotopes; D007553:Isotope Labeling / Q000379:methods*; D009942:Organometallic Compounds; D015125:Oxyquinoline / Q000031:analogs & derivatives; D004369:Pentetic Acid
D006912:Hydroxyquinolines; D007074:Immunoglobulin G; D007205:Indium Radioisotopes; D009942:Organometallic Compounds; C032950:indium oxine; D015125:Oxyquinoline; D004369:Pentetic Acid