Measurement of Pseudomonas aeruginosa phenazine pigments in sputum and assessment of their contribution to sputum sol toxicity for respiratory epithelium.
The phenazine pigments pyocyanin and 1-hydroxyphenazine were resolved by high-pressure liquid chromatography from the sputum sol phase from 9 of 13 patients with cystic fibrosis or bronchiectasis colonized by Pseudomonas aeruginosa. The concentrations measured were each sufficient to inhibit ciliary beating in vitro and contributed a significant proportion of sol phase toxicity for respiratory epithelium.
D002923:Cilia / Q000187:drug effects / Q000382:microbiology; D004848:Epithelium / Q000187:drug effects / Q000382:microbiology; D006801:Humans; D009297:Nasal Mucosa / Q000187:drug effects* / Q000382:microbiology; D010619:Phenazines / Q000032:analysis* / Q000633:toxicity; D010860:Pigments, Biological / Q000032:analysis* / Q000633:toxicity; D011552:Pseudomonas Infections / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000032:analysis*; D013183:Sputum / Q000032:analysis* / Q000382:microbiology
D010619:Phenazines; D010860:Pigments, Biological