The complete sequence of chick apolipoprotein AI mRNA and its expression in the developing chick.
The nucleotide (nt) sequence analysis of a full-length cDNA for chick apolipoprotein AI (Apo-AI) shows an open reading frame (ORF) of 792 nt, coding for a 264-aa protein. RNase mapping and sequence analysis of the 3' end show that apo-AI mRNA consists of at least two different species of 985 and 996 nt, respectively. During the embryonic life of the chick apo-AI mRNA is found in high concentration only in the liver, while its level in the intestine, the major Apo-AI producing organ in the adult, becomes significant only after hatching. This switch from liver to intestine, as primary site of apo-AI mRNA synthesis, takes place about ten days after hatching. The developmental control of the tissue levels of apo-AI mRNA is particularly evident in the skeletal muscle, where this mRNA species is present at high level only immediately after hatching. Preliminary evidence suggests that the time-limited rise in muscle apo-AI mRNA might be due to an increased rate of transcription.
D000595:Amino Acid Sequence; D000818:Animals; D016632:Apolipoprotein A-I; D001054:Apolipoproteins A / Q000235:genetics*; D001483:Base Sequence; D002467:Cell Nucleus / Q000201:enzymology; D002645:Chickens; D003851:Deoxyribonucleases; D004586:Electrophoresis; D008969:Molecular Sequence Data; D009132:Muscles / Q000201:enzymology; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000235:genetics*
D016632:Apolipoprotein A-I; D001054:Apolipoproteins A; D012333:RNA, Messenger; D003851:Deoxyribonucleases