Adenylate cyclase activity in a higher plant, alfalfa (Medicago sativa).
An adenylate cyclase activity in Medicago sativa L. (alfalfa) roots was partially characterized. The enzyme activity remains in the supernatant fluid after centrifugation at 105,000 g and shows in crude extracts an apparent Mr of about 84,000. The enzyme is active with Mg2+ and Ca2+ as bivalent cations, and is inhibited by EGTA and by chlorpromazine. Calmodulin from bovine brain or spinach leaves activates this adenylate cyclase.
D000067956:Adenylyl Cyclase Inhibitors; D000262:Adenylyl Cyclases / Q000378:metabolism*; D002118:Calcium / Q000494:pharmacology; D002147:Calmodulin / Q000494:pharmacology; D002499:Centrifugation, Density Gradient; D002746:Chlorpromazine / Q000494:pharmacology; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D004533:Egtazic Acid / Q000494:pharmacology; D005476:Fluphenazine / Q000494:pharmacology; D000455:Medicago sativa / Q000201:enzymology*
D000067956:Adenylyl Cyclase Inhibitors; D002147:Calmodulin; D004533:Egtazic Acid; D000262:Adenylyl Cyclases; D005476:Fluphenazine; D002118:Calcium; D002746:Chlorpromazine