Induced engulfment of Neisseria gonorrhoeae by tissue culture cells.
Engulfment of gonococci by mammalian tissue culture cells was examined as a model of the penetration of host cells in gonorrhea. Engulfment required viable organisms; killing the gonococci with heat or refrigeration abolished the process. Engulfment also required tissue culture cell microtubule- and microfilament-dependent movement; treating the cells with cytochalasin B (0.5 micrograms/ml) or demecolcine (Colcemid; Ciba-Geigy AG, Basel, Switzerland) (10 micrograms/ml) also prevented his process.
D000818:Animals; D002478:Cells, Cultured; D006224:Cricetinae; D046508:Culture Techniques; D005110:Extracellular Space / Q000382:microbiology; D006367:HeLa Cells; D006801:Humans; D007424:Intracellular Fluid / Q000382:microbiology; D007668:Kidney; D008870:Microtubules / Q000187:drug effects; D009344:Neisseria gonorrhoeae / Q000502:physiology*; D010587:Phagocytosis