title: Amelin: a 4.1-related spectrin-binding protein found in neuronal cell bodies and dendrites.
abstract: An immunoreactive, structural, and functional analog of erythrocyte protein 4.1 is present in neuronal cell bodies and dendrites. Other investigators have described the isolation of a 4.1 analog in brain with structural characteristics suggesting that its identity was synapsin I, a neuronal phosphoprotein localized in the presynaptic terminal in association with small synaptic vesicles. In this report we demonstrate that the cell body/dendritic form of brain protein 4.1, which we have named amelin, is distinct from that of synapsin I on the basis of subcellular localization, migration in 2-dimensional gel electrophoresis, and structural criteria. We also demonstrate that amelin, like synapsin I, can bind brain spectrin on nitrocellulose paper. Neither amelin nor synapsin I binds calmodulin, as determined by a blot binding assay. We hypothesize that there exists in brain a family of 4.1-related proteins with distinct subcellular localization and function.
mesh_terms: D000818:Animals; D001923:Brain Chemistry; D002352:Carrier Proteins / Q000032:analysis; D055598:Chemical Phenomena; D002621:Chemistry; D003712:Dendrites / Q000032:analysis*; D051379:Mice; D008810:Mice, Inbred C57BL; D009419:Nerve Tissue Proteins / Q000032:analysis*; D009474:Neurons / Q000032:analysis*; D016704:Synapsins
chemical_list: D002352:Carrier Proteins; D009419:Nerve Tissue Proteins; D016704:Synapsins; C053624:amelin (neuronal); C025957:spectrin-binding proteins