Expression of the hemolysin trait in a proteolytic transconjugant of Streptococcus faecalis.
Streptococcus faecalis 31H-1 is a proteolytic transconjugant of strain 31B, that harbors the 38.5 Mdal plasmid which codes for hemolysin in strain X14. It fails to express the hemolytic character in broth due to its proteolytic character. Hemolytic activity can be demonstrated when the proteolytic activity is inhibited by 10(-4) M EDTA. The effect of erythromycin (on the donor cells) and of proteinase K during conjugation suggests that the conjugation system involving the 38.5 Mdal plasmid of S. faecalis strain X14 is sex-pherormone mediated.
D003227:Conjugation, Genetic; D013293:Enterococcus faecalis / Q000235:genetics*; D005786:Gene Expression Regulation; D006460:Hemolysin Proteins / Q000235:genetics*; D010447:Peptide Hydrolases / Q000378:metabolism; D010957:Plasmids
D006460:Hemolysin Proteins; D010447:Peptide Hydrolases