Marmoset red blood cell receptor for membrane-associated complement components is not related to human CR1: partial characterization of the C3-binding proteins responsible for the spontaneous rosette formation between marmoset red blood cells and human leukocytes.
Cells from all the human B-lymphoblastoid cell lines tested and most human monocytes form rosettes with marmoset red blood cells (MaRBC). Because previous reports suggested the involvement of complement components in this phenomenon, the mechanism of rosette formation and the eventual similarities between the MaRBC receptor and the CR1 receptor present on human erythrocytes have been analyzed herein. The binding of MaRBC to human leukocytes strongly differs from the immune adherence phenomenon: rabbit anti-human CR1 did not react with MaRBC and the MaRBC receptor-binding activity is Ca2+-dependent. Rosette formation required intact energy metabolism and cytoskeleton integrity of leukocytes. Our attempts to purify the receptor from MaRBC membranes revealed the absence of CR1. Nevertheless, C3-binding proteins were isolated by selective desorption by Sepharose iC3 column chromatography. A three-band pattern was observed under reduced conditions with 74,000, 70,000, and 53,000 molecular weights. It was not possible to further separate these components. This protein complex inhibited the rosette phenomenon between MaRBC and both Raji and U-937 cells, exhibited a very poor cofactor activity, and had no decay-accelerating activity toward the classical C3 convertase. This material did not cross-react with antibodies directed to human proteins. These results showed that erythrocytes from new world monkeys do not express a receptor analogous to the human CR1, but expressed C3-binding protein with low cofactor activity that could recognize membrane-associated complement components.
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002143:Callitrichinae / Q000276:immunology*; D002460:Cell Line; D004910:Erythrocyte Membrane / Q000276:immunology*; D006801:Humans; D007700:Kinetics; D007962:Leukocytes / Q000276:immunology*; D008274:Magnesium / Q000494:pharmacology; D008970:Molecular Weight; D011951:Receptors, Complement / Q000276:immunology* / Q000302:isolation & purification; D012397:Rosette Formation; D013045:Species Specificity
D011951:Receptors, Complement; D008274:Magnesium; D002118:Calcium