The production and radioimmunoassay application of monoclonal antibodies to fluorescein isothiocyanate (FITC).
Monoclonal antibodies (MoAbs) were produced against the fluorescence marker fluorescein isothiocyanate (FITC). FITC was used as a hapten to label different proteins and the anti-FITC MoAbs were used to identify these labelled proteins in a solid-phase radioimmunoassay and in cellular radioimmuno-binding assays for the demonstration of antigens and antibodies.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000918:Antibody Specificity; D001666:Binding Sites, Antibody; D002459:Cell Fusion; D002460:Cell Line; D006063:Chorionic Gonadotropin / Q000032:analysis / Q000276:immunology; D003110:Colonic Neoplasms / Q000032:analysis; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins / Q000276:immunology*; D006241:Haptens / Q000276:immunology; D006801:Humans; D007202:Indicators and Reagents; D051379:Mice; D008807:Mice, Inbred BALB C; D011863:Radioimmunoassay / Q000379:methods*; D013861:Thiocyanates / Q000276:immunology*
D000911:Antibodies, Monoclonal; D006063:Chorionic Gonadotropin; D005452:Fluoresceins; D006241:Haptens; D007202:Indicators and Reagents; D013861:Thiocyanates; D016650:Fluorescein-5-isothiocyanate