Modulation of plasminogen activation by glucocorticoid hormones in the rat granulosa cell.
Granulosa cells from immature rats primed with diethylstilbestrol (DES) showed a fivefold rise in the activity of secreted plasminogen activator (PA) in response to human FSH. The dose giving 50% of the maximum response (ED50) was 10 ng/ml. Glucocorticoid hormones significantly suppressed PA activity in both control cells and cells stimulated with FSH (ED50 = 50 nmol dexamethasone/l; 1 mumol corticosterone/l). Cortexolone (2.5 mumol/l) significantly ameliorated this suppression, indicating that the response to glucocorticoids is receptor-mediated. These data, together with the time delay required for glucocorticoids to take effect (5 h), suggest that glucocorticoids induce the production of a specific PA inhibitor in granulosa cells.
D000818:Animals; D003345:Corticosterone / Q000494:pharmacology; D003350:Cortodoxone / Q000494:pharmacology; D003907:Dexamethasone / Q000494:pharmacology; D004054:Diethylstilbestrol / Q000494:pharmacology; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology*; D006107:Granulosa Cells / Q000187:drug effects* / Q000378:metabolism; D010960:Plasminogen Activators / Q000037:antagonists & inhibitors / Q000378:metabolism*; D015849:Plasminogen Inactivators; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
D015849:Plasminogen Inactivators; D004054:Diethylstilbestrol; D003907:Dexamethasone; D005640:Follicle Stimulating Hormone; D010960:Plasminogen Activators; D003345:Corticosterone; D003350:Cortodoxone