Experimental evidence for enteropathogenicity in Aeromonas veronii.
Eleven ornithine-positive strains of Aeromonas (9 A. veronii and 2 provisionally classified as Aeromonas species ornithine positive) were tested for ability to cause fluid accumulation in the rabbit ileal loop. All eight beta-hemolytic strains caused fluid accumulation. Gel diffusion analysis revealed that the A. veronii beta-hemolysin was serologically related to the A. hydrophila beta-hemolysin, a known enterotoxic molecule. The biological activity of the A. veronii hemolysin was neutralized by antiserum to A. hydrophila hemolysin. One of three strains that were not beta-hemolytic caused fluid accumulation but only when the ileal loops were inoculated with live cultures. These results suggest that A. veronii is a potential enteropathogen that can cause diarrhea by means of a cell-freed enterotoxin (beta-hemolysin) or by a second mechanism that requires the presence of whole cells.
D000333:Aeromonas / Q000378:metabolism / Q000472:pathogenicity*; D000818:Animals; D001424:Bacterial Infections / Q000382:microbiology*; D003967:Diarrhea / Q000382:microbiology*; D006460:Hemolysin Proteins / Q000096:biosynthesis; D007082:Ileum / Q000382:microbiology*; D005779:Immunodiffusion; D011817:Rabbits
D006460:Hemolysin Proteins