Physiological roles of two enzymes with fumarase activity in two Pseudomonads.
Effects of Fe2+ ions on the levels of two enzymes (fumarase and mesaconase) with fumarase activity in two Pseudomonads grown under various nutritional conditions were investigated. Fe2+ ions decreased fumarase but increased mesaconase. A high level of mesaconase was found in Ps. arvilla which was unable to metabolize itaconate. The level of mesaconase in the itaconate-grown cells of Ps. fluorescens was almost the same as that in the glucose-grown cells. This suggests that mesaconase is not an enzyme involved in the metabolism of C5-branched-chain dicarboxylates but presumably, taking the place of fumarase, plays a role in the operation of the tricarboxylic acid cycle in the cells grown in the medium containing Fe2+ ions more than 10 nmol/ml.
D005296:Ferrous Compounds / Q000494:pharmacology*; D005649:Fumarate Hydratase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D006836:Hydro-Lyases / Q000378:metabolism*; D011549:Pseudomonas / Q000201:enzymology* / Q000254:growth & development; D011551:Pseudomonas fluorescens / Q000201:enzymology / Q000254:growth & development
D005296:Ferrous Compounds; C020748:ferrous sulfate; D006836:Hydro-Lyases; D005649:Fumarate Hydratase; C021552:(S)-2-methylmalate dehydratase