A new aspect of the protective functions of the blood-brain barrier: activities of four drug-metabolizing enzymes in isolated rat brain microvessels.
The multiple functions of the blood-brain barrier (BBB) consist mostly in membrane properties controlling the bidirectional exchange of molecules between the general circulation and the central nervous system. As lipophilic molecules are able to easily penetrate the membrane of brain microvessels endothelial cells, an Achilles' heel exists in the brain's protective system. We measured the activities of some enzymes involved in the metabolism of lipophilic xenobiotics, i.e. cytochrome P-450-linked monooxygenases, epoxide hydrolase, NADPH:cytochrome P-450 reductase and 1-naphthol UDP-glucuronosyl transferase in isolated rat brain microvessels. The relatively high activities observed indicate the capacity of endothelial cells to metabolize xenobiotics, and, thus, to give an additional protection to the brain.
D000818:Animals; D001812:Blood-Brain Barrier; D001921:Brain / Q000098:blood supply*; D002196:Capillaries / Q000201:enzymology; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D004851:Epoxide Hydrolases / Q000378:metabolism*; D014453:Glucuronosyltransferase / Q000378:metabolism*; D008297:Male; D009251:NADPH-Ferrihemoprotein Reductase / Q000378:metabolism*; D009284:Naphthols / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D009284:Naphthols; C029350:1-naphthol; D003577:Cytochrome P-450 Enzyme System; D009251:NADPH-Ferrihemoprotein Reductase; D014453:Glucuronosyltransferase; D004851:Epoxide Hydrolases