Efficiency of translation initiation by non-AUG codons in Saccharomyces cerevisiae.
The quantitative levels of initiation of protein synthesis at codons other than AUG were determined with a CYC7-lacZ fused gene in the yeast Saccharomyces cerevisiae. AUG was the only codon which efficiently initiated translation, although some non-AUG codons allowed initiation at very low efficiency, below 1% of the normal level. Since translation initiates at codons other than AUG in at least two wild-type genes from eucaryotes, other factors presumably play a role in enhancing the activity of non-AUG codons.
D001483:Base Sequence; D003001:Cloning, Molecular; D003062:Codon; D004252:DNA Mutational Analysis; D008969:Molecular Sequence Data; D010442:Peptide Chain Initiation, Translational; D012331:RNA, Fungal / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014158:Transcription, Genetic; D001616:beta-Galactosidase / Q000235:genetics
D003062:Codon; D012331:RNA, Fungal; D012333:RNA, Messenger; D001616:beta-Galactosidase