200 Kd neurofilament protein binds Al, Cu and Zn.
200 Kd human and bovine neurofilament proteins were isolated from spinal cord and purified to homogeneity. The purified proteins were shown to be metal-binding proteins which bind at least one mole of Al, one mole of Cu and four moles of Zn. Neither dephosphorylation of the protein nor equilibrium dialysis against 25 mM EDTA, pH 7.0, removed these metals. This is the first report of a human protein which stoichiometrically binds Al.
D000535:Aluminum / Q000378:metabolism; D000818:Animals; D002352:Carrier Proteins / Q000302:isolation & purification / Q000378:metabolism*; D002417:Cattle; D003300:Copper / Q000378:metabolism; D006801:Humans; D007381:Intermediate Filament Proteins / Q000302:isolation & purification / Q000378:metabolism*; D008670:Metals / Q000378:metabolism*; D008970:Molecular Weight; D016900:Neurofilament Proteins; D013116:Spinal Cord; D015032:Zinc / Q000378:metabolism
D002352:Carrier Proteins; D007381:Intermediate Filament Proteins; D008670:Metals; D016900:Neurofilament Proteins; D003300:Copper; D000535:Aluminum; D015032:Zinc