Analysis of delta mRNA in the immature B lymphocytes of neonatal liver and spleen.
The production of mu m and delta m mRNA was assessed by measuring the relative levels of the two species in nuclear RNA isolated from neonatal liver and from spleen cells obtained from mice of differing ages. These analyses showed that the lack of IgD expression in the immature B cells of neonatal liver is due to an absence of delta mRNA. The presence of high mol. wt species which hybridize to a delta probe indicates that the delta coding regions are transcribed and suggest that post-transcriptional events account for the lack of delta expression. Little change in the mu m-delta m mRNA ratio was observed in nuclear RNA isolated from spleen cells of mice aged from 5 days to 4 months. Thus, variations in the levels of mRNA synthesis play an insignificant role in the changes in IgM and IgD expression seen during B cell maturation in neonatal spleen.
D000375:Aging; D000818:Animals; D000831:Animals, Newborn / Q000276:immunology*; D001402:B-Lymphocytes / Q000032:analysis* / Q000276:immunology; D004587:Electrophoresis, Agar Gel; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D007138:Immunoglobulin delta-Chains / Q000235:genetics*; D007148:Immunoglobulin mu-Chains / Q000235:genetics; D008099:Liver / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D012333:RNA, Messenger / Q000032:analysis*; D013154:Spleen / Q000276:immunology*
D007143:Immunoglobulin Heavy Chains; D007138:Immunoglobulin delta-Chains; D007148:Immunoglobulin mu-Chains; D012333:RNA, Messenger