Correlation of in vitro immune defects with impaired gamma interferon response in human-immunodeficiency-virus-infected individuals.
65 patients belonging to known acquired-immune-deficiency-syndrome (AIDS) risk groups were tested for mitogen responsiveness to pokeweed mitogen, concanavalin A and phytohemagglutinin, percentages of peripheral blood CD4, CD8 and Leu7 lymphocyte subsets, serum neopterin levels, and gamma-interferon (gamma-IFN) concentrations in cell culture supernatants. Patients with clinical symptoms of human-immunodeficiency-virus infection showed reduced gamma-IFN production in vitro. The IFN concentrations were correlated significantly with mitogen-induced blastogenesis. High serum neopterin levels and decreased CD4/CD8 ratios were associated with diminished gamma-IFN levels. The percentage of Leu7 lymphocytes was increased in a group of patients with strikingly elevated gamma-IFN levels.
D000163:Acquired Immunodeficiency Syndrome / Q000097:blood / Q000276:immunology*; D000945:Antigens, Differentiation, T-Lymphocyte; D001708:Biopterins / Q000031:analogs & derivatives / Q000097:blood; D006801:Humans; D066298:In Vitro Techniques; D007371:Interferon-gamma / Q000096:biosynthesis*; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000145:classification / Q000276:immunology; D008934:Mitogens / Q000494:pharmacology; D019798:Neopterin
D000945:Antigens, Differentiation, T-Lymphocyte; D008934:Mitogens; D001708:Biopterins; D019798:Neopterin; D007371:Interferon-gamma