Juvenile hormone action mediated in male accessory glands of Drosophila by calcium and kinase C.
In an in vitro system for the Drosophila melanogaster male accessory gland, it was found that 10(-9)M juvenile hormone III could accurately mimic the copulation-induced response of increased protein synthesis in glands from virgin flies. Stimulation by this hormone required calcium in the medium. Experiments with tumor-promoting phorbol esters indicated that activation of protein kinase C can also cause the glands to increase protein synthesis. Stimulation of protein synthesis by juvenile hormone did not occur in mutants deficient in kinase C activity. These results suggest a membrane-protein-mediated effect of juvenile hormone that involves calcium and kinase C.
D000818:Animals; D002118:Calcium / Q000502:physiology*; D004331:Drosophila melanogaster / Q000378:metabolism*; D004789:Enzyme Activation / Q000187:drug effects; D005837:Genitalia, Male / Q000187:drug effects / Q000378:metabolism; D007605:Juvenile Hormones / Q000235:genetics; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism; D009154:Mutation; D015240:Phorbol 12,13-Dibutyrate; D010703:Phorbol Esters / Q000494:pharmacology; D014176:Protein Biosynthesis; D011493:Protein Kinase C / Q000378:metabolism*; D012717:Sesquiterpenes / Q000494:pharmacology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D007605:Juvenile Hormones; D008565:Membrane Proteins; D010703:Phorbol Esters; D012717:Sesquiterpenes; D015240:Phorbol 12,13-Dibutyrate; C036585:juvenile hormone III; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium