title: Autoclaved, formol-fixed scrapie mouse brain is suitable for histopathological examination, but may still be infective.
abstract: Autoclaving Creutzfeldt-Jakob disease (CJD)-infected brain after fixation has been proposed previously as a means of making it safe for handling in the laboratory, while preserving its microscopic integrity. However, the practice which was recommended (126 degrees C for 30 min) would be unlikely to achieve complete decontamination. Autoclaving scrapie mouse brain (an analogue for CJD) to the higher, recommended standard of 134-138 degrees C for 18 min was found to lead to some tissue damage but not sufficient to prevent meaningful qualitative and quantitative study of the histopathological lesions.
mesh_terms: D000818:Animals; D001921:Brain / Q000382:microbiology* / Q000473:pathology; D005404:Fixatives; D005557:Formaldehyde; D006358:Hot Temperature; D051379:Mice; D008809:Mice, Inbred C3H; D011328:Prions / Q000472:pathogenicity*; D012608:Scrapie / Q000382:microbiology* / Q000473:pathology; D013242:Sterilization / Q000379:methods*
chemical_list: D005404:Fixatives; D011328:Prions; D005557:Formaldehyde