[The binding behavior and functional significance of Fc receptors for IgG on T lymphocytes of the mouse].
In consequence of allogenic stimulation of mouse spleen lymphocytes the binding of fluorescein isothiocyanate conjugated aggregated IgG (FITC-aggr. IgG) per cell is increased (higher affinity of receptors and/or higher number of receptors). A higher specificity of the FITC-aggr. IgG binding to allogeneically stimulated lymphocytes in comparison to fresh prepared resting and syngeneically cultivated lymphocytes could be only detected on the higher fluorescing allogeneically stimulated cells. Increased amount of Fc-receptors for IgG (Fc gamma R) is mainly found on T-lymphocytes with the Lyt-1 + 2 +-phenotype. The manipulation of that Fc gamma R on T-lymphocytes, which were expressed in consequence of allogeneic stimulation by binding of respective ligands, did not change the original effect of nonmanipulated, allogeneically activated cells on a primary MLR. But if the cells were negatively selected by means of monoclonal antibodies and complement, the originally suppressive effect of allogeneically stimulated Lyt-2 positive cells on a primary MLR was abolished by manipulating the respective Fc gamma R.
D000818:Animals; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D007074:Immunoglobulin G / Q000378:metabolism*; D051379:Mice; D008815:Mice, Inbred Strains; D011961:Receptors, Fc / Q000378:metabolism*; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000276:immunology*; D013861:Thiocyanates
D005452:Fluoresceins; D007074:Immunoglobulin G; D011961:Receptors, Fc; D013861:Thiocyanates; D016650:Fluorescein-5-isothiocyanate
Untersuchungen zum Bindungsverhalten und zur funktionellen Bedeutung von Fc-Rezeptoren für IgG auf T-Lymphozyten der Maus.