Immunocytochemical and ultrastructural characterization of coexistence of pancreatic polypeptide and glucagon-like immunoreactivity in the pancreatic endocrine cells of Sparus auratus L. (Teleostei).
Coexistence of pancreatic polypeptide (PP)- and glucagon-like immunoreactivity was demonstrated in the pancreatic endocrine cells of the teleost fish Sparus auratus. An immunofluorescence double-staining method revealed coexistence of glucagon- and PP-like immunoreactivity in endocrine cells of small and intermediate islets. In contrast to small islets, the intermediate ones also contained a variable number of glucagon-immunoreactive cells next to cells having both immunoreactivities. Coexistence of both immunoreactivities could not be observed in endocrine cells of the principal islet, whereas many cells containing glucagon and a few cells containing PP immunoreactivity were found. By an immunogold double-staining method the precise ultrastructural location of each immunoreactivity could be demonstrated. Again, cells containing glucagon- and/or PP-like immunoreactivity were found. Although, only two different types of granules were observed, four distinct cell types could be distinguished. Based on this granule morphology two cell types showing coexistence were found: one cell type, only present in the small islets, showing a different distribution of glucagon and PP immunoreactivity within the granules (predominantly in the center and periphery, respectively) and another cell type with larger granule cores, present in small as well as intermediate islets, having a mixed distribution of both immunoreactivities.
D000818:Animals; D003594:Cytoplasmic Granules / Q000648:ultrastructure; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes; D005934:Glucagon / Q000032:analysis*; D006651:Histocytochemistry; D007150:Immunohistochemistry; D007515:Islets of Langerhans / Q000032:analysis* / Q000648:ultrastructure; D008854:Microscopy, Electron; D010191:Pancreatic Polypeptide / Q000032:analysis*; D010473:Perciformes / Q000378:metabolism*; D012235:Rhodamines; D013861:Thiocyanates
D005452:Fluoresceins; D005456:Fluorescent Dyes; D012235:Rhodamines; D013861:Thiocyanates; C009434:tetramethylrhodamine isothiocyanate; D010191:Pancreatic Polypeptide; D005934:Glucagon; D016650:Fluorescein-5-isothiocyanate