[Solid phase radioimmunoassay for the quantitative determination of antigen-specific IgG-antibodies in the serum of patients with farmer's lung].
In this study we have investigated a number of technical aspects of a sensitive solid-phase radioimmunoassay (microtitre-plate RIA) for the quantitative determination of antigen-specific IgG antibodies in the sera of patients suffering from farmer's lung. Microtitre-plates were coated with antigens of Aspergillus fumigatus, Micropolyspora faeni, Aureobasidium pullulans, Alternaria tenuis, Thermoactinomyces vulgaris and hay. Optimal antigen concentration and serum dilution were determined, the temperature-dependence of the assay and the kinetics of the binding of antibodies and protein A were studied. A comparison between glutaraldehyde-activated and non-activated microtitre-plates showed that the RIA with activated microtitre-plates produces values approximately 10-times higher than those with non-activated plates. However, the use of activated plates did not significantly increase the ratio between patient and control values.
D000917:Antibody Formation; D000937:Antigen-Antibody Reactions; D005203:Farmer's Lung / Q000276:immunology*; D005976:Glutaral / Q000378:metabolism; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis*; D007700:Kinetics; D011863:Radioimmunoassay; D013205:Staphylococcal Protein A / Q000276:immunology; D013696:Temperature
D007074:Immunoglobulin G; D013205:Staphylococcal Protein A; D005976:Glutaral
Festphasen-Radioimmunoassay zur quantitativen Bestimmung von antigenspezifischen IgG-Antikörpern im Serum von Patienten mit Farmerlunge.