Modulation of some Peyer's patch leukocyte functions following in vitro exposure to recombinant bovine alpha- and gamma-interferon.
The immunomodulatory effects of recombinant bovine interferon-alpha 1(1) (rBoIFN-alpha 1(1] and -gamma (rBoIFN-gamma) on the response of bovine Peyer's patch leukocytes (PPL) to in vitro mitogenic stimulation were studied. The proliferative response of PPL to concanavalin A (Con A) and pokeweed mitogen (PWM) was significantly inhibited by the addition of 5-1000 units/ml of rBoIFN-alpha 1(1). In contrast, rBoIFN-gamma showed significant enhancement of 3H-Thymidine (3HTdR) incorporation of PPL cultures at concentrations of 5-500 units/ml. Although the results of this study substantiated the previously reported suppressive ability of IFN-alpha 1(1) and the stimulating capacity of IFN-gamma, suppression or enhancement of lymphocyte proliferation was shown to be dependent on the concentration of the polyclonal activator and the length of time the target cells were exposed to IFN. Pre-exposure of target cells to rBoIFN-alpha 1(1) significantly increased its suppressive activity against the PPL response to Con A stimulation. In contrast, pre-exposure of PPL to rBoIFN-gamma did not affect its stimulatory capacity. Prestimulation of target cells with Con A significantly decreased the stimulatory capacity of rBoIFN-gamma and the suppressive activity of rBoIFN-alpha 1(1). These data demonstrate qualitative differences in the effects of rBoIFN-alpha 1(1) compared to those of rBoIFN-gamma on immune cells other than commonly studied circulating leukocytes.
D000276:Adjuvants, Immunologic; D000818:Animals; D002417:Cattle; D003208:Concanavalin A / Q000494:pharmacology; D004247:DNA / Q000096:biosynthesis; D066298:In Vitro Techniques; D007370:Interferon Type I / Q000494:pharmacology*; D007371:Interferon-gamma / Q000494:pharmacology*; D007700:Kinetics; D007962:Leukocytes / Q000276:immunology*; D008213:Lymphocyte Activation; D010581:Peyer's Patches / Q000276:immunology*; D011043:Pokeweed Mitogens / Q000494:pharmacology
D000276:Adjuvants, Immunologic; D007370:Interferon Type I; D011043:Pokeweed Mitogens; D003208:Concanavalin A; D007371:Interferon-gamma; D004247:DNA