Antibody inhibition of HeLa cell invasion by Yersinia enterocolitica.
Antisera were prepared in rabbits against formalized and heat-killed bacteria of Yersinia enterocolitica serotype O:5,27 and against formalized bacteria of serotype O:8. Both strains used for immunization demonstrated adhesion to and invasion of HeLa cells. Coating of the bacteria with antibody did not greatly alter adhesion (i.e., extracellular attachment) to HeLa cells; however, antibody against formalized bacteria of both serotypes inhibited HeLa cell invasion by the homologous and heterologous strains. The Fab fragments from purified immunoglobulins also demonstrated cross-reacting inhibition of HeLa cell invasion. Antibody against heat-killed bacteria of serotype O:5,27 had no inhibitory activity. Adsorption of the antiserum against formalized bacteria of serotype O:5,27 with lipopolysaccharide from the homologous strain removed anti-lipopolysaccharide antibody but did not remove the inhibitory activity. The antiserum against formalized bacteria of serotype O:8 showed no antibody against lipopolysaccharide from serotype O:5,27 and no agglutinins against heat-killed bacteria of this strain. From these results, it is tentatively suggested that protein structures are important in mediating epithelial cell invasion by Y. enterocolitica.
D000327:Adsorption; D000942:Antigens, Bacterial / Q000276:immunology; D001422:Bacterial Adhesion; D006367:HeLa Cells / Q000382:microbiology; D006358:Hot Temperature; D006801:Humans; D007106:Immune Sera / Q000276:immunology*; D005779:Immunodiffusion; D007140:Immunoglobulin Fab Fragments / Q000276:immunology; D008070:Lipopolysaccharides / Q000276:immunology; D015008:Yersinia enterocolitica / Q000276:immunology* / Q000502:physiology
D000942:Antigens, Bacterial; D007106:Immune Sera; D007140:Immunoglobulin Fab Fragments; D008070:Lipopolysaccharides