Reversal of the effects of cyclosporin A on the production of human interferon-gamma.
Previous reports have documented that cyclosporin A (CsA) can inhibit the production of interferon-gamma (HuIFN-gamma) and interleukin-2 (IL-2). In this report we have extended these earlier findings and have demonstrated that the defect in the production of HuIFN-gamma persists even after the CsA is removed and is associated with altered synthesis of other lymphokines, mainly a cytotoxin and leukocyte migration inhibition factor. In the presence of recombinant exogenous IL-2, the effect of CsA on the synthesis of HuIFN-gamma can be reversed depending on the concentrations of both the IL-2 and CsA. In addition, the presence of the calcium ionophore A23187 partially reverses the inhibitory effect of CsA by synergistically enhancing the synthesis of HuIFN-gamma.
D000001:Calcimycin / Q000494:pharmacology; D003524:Cyclosporins / Q000494:pharmacology*; D003603:Cytotoxins / Q000096:biosynthesis; D004347:Drug Interactions; D006801:Humans; D066298:In Vitro Techniques; D007371:Interferon-gamma / Q000096:biosynthesis*; D007376:Interleukin-2 / Q000494:pharmacology; D007961:Leukocyte Migration-Inhibitory Factors / Q000096:biosynthesis; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000276:immunology; D008222:Lymphokines / Q000096:biosynthesis; D010835:Phytohemagglutinins / Q000494:pharmacology
D003524:Cyclosporins; D003603:Cytotoxins; D007376:Interleukin-2; D007961:Leukocyte Migration-Inhibitory Factors; D008222:Lymphokines; D010835:Phytohemagglutinins; D000001:Calcimycin; D007371:Interferon-gamma