Assessment of the mutagenicity of fractions from s-triazine-treated Zea mays.
A water-soluble extract from maize plants exposed to 3 s-triazine herbicides (atrazine, simazine and cyanazine) has been shown to be mutagenic in strain TA100 of Salmonella. No mutagenic activity was observed in any control plant extracts using either water or a variety of organic solvents. Gel permeation studies of the extracts suggest that the mutagen(s) are small molecules (less than 1000 MW). HPLC fractionation suggests that the mutagens formed from each of the 3 herbicides are similar in polarity and water solubility, eluting in a 50/50 water:methanol fraction. Approximately 89% of 14C-labeled HPLC chromatographable metabolites of atrazine were also associated with this fraction, suggesting a close chemical link between a labeled but unidentified metabolite and the mutagenic activity.
D001280:Atrazine / Q000378:metabolism / Q000494:pharmacology*; D001412:Bacillus subtilis / Q000187:drug effects*; D001711:Biotransformation; D002458:Cell Fractionation; D002851:Chromatography, High Pressure Liquid; D006540:Herbicides / Q000494:pharmacology*; D009152:Mutagenicity Tests; D009153:Mutagens / Q000096:biosynthesis / Q000494:pharmacology; D010936:Plant Extracts / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000187:drug effects*; D012486:Salmonella typhimurium / Q000187:drug effects*; D012839:Simazine / Q000378:metabolism / Q000494:pharmacology*; D012995:Solubility; D014227:Triazines / Q000378:metabolism / Q000494:pharmacology*; D003313:Zea mays / Q000378:metabolism*
D006540:Herbicides; D009153:Mutagens; D010936:Plant Extracts; D014227:Triazines; D001280:Atrazine; D012839:Simazine; C007168:cyanazine