A new rare distamycin A-inducible fragile site, fra(11) (p15.1), found in two acute nonlymphocytic leukemia (ANLL) patients with t(7;11)(p15-p13;p15).
Fragile sites were analyzed in normal peripheral lymphocytes from two acute nonlymphocytic leukemia patients with t(7;11)(p15-p13;p15) leukemic cells. To induce expression of fragile sites, cultures were exposed to folate deprivation (M-F10), BrdU, distamycin A, or Hoechst 33258. Fragility at 11p15.1 was induced by distamycin A and Hoechst 33258 but was not seen in M-F10, BrdU, and control cultures. Fra(11)(p15.1) was found neither in healthy Japanese subjects (0 in 845) nor in patients with leukemia or other hematologic disorders without the t(7;11) (0 in 126). From these results, fra(11)(p15.1) can now be classified as a rare distamycin A-inducible fragile site. Furthermore, this fra(11)(p15.1) coincided with one of the breakpoints of the t(7;11)(p15-p13;p15).
Takahashi
E
E
Division of Genetics, National Institute of Radiological Sciences, Chiba, Japan.
Kaneko
Y
Y
Ishihara
T
T
Minamihisamatsu
M
M
Murata
M
M
Hori
T
T
D000293:Adolescent; D043283:Chromosome Fragile Sites; D002873:Chromosome Fragility; D002880:Chromosomes, Human, Pair 11; D002897:Chromosomes, Human, Pair 7; D004214:Distamycins / Q000494:pharmacology; D005260:Female; D005819:Genetic Markers; D006801:Humans; D007621:Karyotyping; D015470:Leukemia, Myeloid, Acute / Q000235:genetics*; D008214:Lymphocytes / Q000648:ultrastructure; D008297:Male; D008875:Middle Aged; D014178:Translocation, Genetic
D004214:Distamycins; D005819:Genetic Markers; C030000:stallimycin