title: Mitogenic response of cells in culture to demineralized bone matrix.
abstract: In this study the mitogenic effect of demineralized bone powder (DBP) on cells in tissue culture was evaluated. The cells studied have osteoblastic properties. At a DBP concentration of 100 micrograms/well, rat osteosarcoma cells (ROS) 17/2.8 cells, ROS 24/1 cells, and periosteal fibroblasts (POF) all showed a significant increase in proliferation in comparison to controls. The peak effect was on day two for ROS 17/2.8 (125% of control), on day three for ROS 24/1 (139% of control), and on day four for POF (145% of control). The results indicate that this in vitro system may be useful for evaluating the osteoinductive activity of DBP preparations.
authors.forename: D S
authors.initials: DS
authors.lastname: Huang
authors.forename: R T
authors.initials: RT
authors.lastname: Kao
authors.forename: A
authors.initials: A
authors.lastname: Shteyer
authors.forename: L
authors.initials: L
authors.lastname: Kaban
mesh_terms: D000818:Animals; D001857:Bone Matrix / Q000378:metabolism / Q000502:physiology*; D002452:Cell Count; D002455:Cell Division; D003470:Culture Media; D046508:Culture Techniques / Q000379:methods; D005069:Evaluation Studies as Topic; D005347:Fibroblasts / Q000502:physiology*; D008903:Minerals / Q000378:metabolism*; D010006:Osteoblasts / Q000502:physiology*; D010012:Osteogenesis; D012516:Osteosarcoma; D051381:Rats; D014407:Tumor Cells, Cultured
chemical_list: D003470:Culture Media; D008903:Minerals