title: Stimulation of rabbit synoviocyte prostaglandin E2 synthesis by lipopolysaccharides and their subunit structures.
abstract: Lipopolysaccharides (LPS) induce synoviocyte activation and may lead to destruction of synovial joint tissues. We assessed the production of prostaglandin E2 (PGE2) as a measure of synoviocyte activation by LPS and their subunit structures. Diphosphoryl lipid A was the smallest portion of lipid A tested that stimulated PGE2 production. The polysaccharide fraction of LPS, containing the O antigen, was also active. Intraarticular injections of the polysaccharide resulted in a synovitis very similar to that found in association with the intact LPS molecule. These observations suggest that both parts of LPS might be involved in gram-negative, organism-associated synovitis.
authors.affiliation: Department of Pathology, William S. Middleton Memorial Veterans Hospital, Madison, Wisconsin 53705.
authors.forename: R J
authors.initials: RJ
authors.lastname: Rothenberg
authors.forename: D
authors.initials: D
authors.lastname: England
authors.forename: N
authors.initials: N
authors.lastname: Qureshi
authors.forename: K
authors.initials: K
authors.lastname: Takayama
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D015232:Dinoprostone; D004305:Dose-Response Relationship, Drug; D007719:Knee Joint / Q000473:pathology; D008070:Lipopolysaccharides / Q000008:administration & dosage / Q000494:pharmacology*; D011134:Polysaccharides / Q000502:physiology; D011458:Prostaglandins E / Q000096:biosynthesis*; D011817:Rabbits; D013582:Synovial Fluid / Q000166:cytology*; D013583:Synovial Membrane / Q000473:pathology
chemical_list: D008070:Lipopolysaccharides; D011134:Polysaccharides; D011458:Prostaglandins E; D015232:Dinoprostone