N-ras gene activation in the RD human rhabdomyosarcoma cell line.
A transforming N-ras allele was molecularly cloned from the RD human rhabdomyosarcoma cell line, and the nature of its activation studied. Construction of chimeric recombinants between the RD-transforming allele and a normal human allele enabled us to localize the alteration responsible for the activation to the second exon of the N-ras gene. The nucleotide sequence of this exon, when compared to that of the normal allele, revealed a single difference at the 61st amino acid position of the encoded protein; the CAA codon for glutamine in the normal allele was mutated to a CAT codon for histidine in the RD-transforming allele. This result is the first description of a histidine replacing glutamine in the 61st position and provides further evidence that the 61st amino acid is one of the preferential sites for N-ras activation.
Chardin
P
P
Yeramian
P
P
Madaule
P
P
Tavitian
A
A
D000483:Alleles; D010582:Bacteriophage lambda / Q000235:genetics; D001483:Base Sequence; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002678:Chimera; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics; D006801:Humans; D009857:Oncogenes; D012208:Rhabdomyosarcoma / Q000235:genetics*; D014162:Transfection
D004279:DNA, Viral