title: Difference in the production of human interferon-alpha and -beta in mouse cells.
abstract: Human interferon-alpha 1 and interferon-beta genes with their flanking regions were introduced into mouse LMTK- cells. Although transfected cells contained the interferon genes with a similar copy number and produced a similar amount of interferon-specific mRNA, cells containing the human interferon-beta gene secreted about 10 times more human interferon than cells transfected with the human interferon-alpha 1 gene. When the coding region of the interferon-beta gene was replaced by that of the interferon-alpha 1 gene (hybrid interferon beta/alpha gene), the human interferon production of transfected cells fell by approx. one order of magnitude. These results show that in the case of exogenous interferon genes a translational or post-translational mechanism might significantly affect the final level of human interferons, resulting in higher titres of interferon-beta than of interferon-alpha.
authors.affiliation: Institute of Biochemistry, Hungarian Academy of Sciences, Szeged.
authors.forename: M I
authors.initials: MI
authors.lastname: Toth
authors.forename: O
authors.initials: O
authors.lastname: Cendsuren
authors.forename: V
authors.initials: V
authors.lastname: Endresz
authors.forename: I
authors.initials: I
authors.lastname: Karcagi
authors.forename: E
authors.initials: E
authors.lastname: Duda
mesh_terms: D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D002999:Clone Cells; D005786:Gene Expression Regulation; D005796:Genes; D006801:Humans; D007370:Interferon Type I / Q000096:biosynthesis* / Q000235:genetics; D051379:Mice; D010957:Plasmids; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis*; D015183:Restriction Mapping; D014170:Transformation, Genetic
chemical_list: D007370:Interferon Type I; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins