Assay for detection of adenosine phosphorylase from mycoplasmas.
A microtechnique is described which permits simple evaluation of the activity of the enzyme adenosine phosphorylase (AdoP), present in all mycoplasmas tested thus far. The good solubility and stability of AdoP and the sensitivity of the assay should be advantageous in detecting mycoplasmas in biological samples such as animal sera, cell cultures and vaccines.
Bonissol
C
C
Laboratoire des Mycoplasmes, Institut Pasteur, Paris.
Sasaki
T
T
Stoiljkovic
B
B
D000241:Adenosine / Q000378:metabolism; D002498:Centrifugation; D004355:Drug Stability; D007700:Kinetics; D008832:Microchemistry; D009174:Mycoplasma / Q000201:enzymology* / Q000254:growth & development; D010430:Pentosyltransferases / Q000032:analysis*; D011683:Purine-Nucleoside Phosphorylase / Q000032:analysis* / Q000096:biosynthesis; D013010:Sonication; D013045:Species Specificity; D013696:Temperature; D014462:Ultrafiltration
D010430:Pentosyltransferases; C019603:adenosine phosphorylase; D011683:Purine-Nucleoside Phosphorylase; D000241:Adenosine