The cloning of the rorB gene of Escherichia coli.
A segment of the Escherichia coli genome which complements the ionising radiation sensitivity of the rorB mutation was cloned into pBR322. This DNA segment also complements the mitomycin C sensitivity of the rorB mutation. The gene was subcloned until defined in a fragment of 1.05 kb. Only one gene product, a protein of approximately 16.5 kDa, was found on maxicell analysis of the various subclones. Iso-electric focusing of this gene product suggests it may function in a complex.
Debenham
P G
PG
Division of Cell and Molecular Biology, M.R.C. Radiobiology Unit, Didcot, Oxon, UK.
Webb
M B
MB
Law
J
J
D003001:Cloning, Molecular; D004260:DNA Repair / Q000187:drug effects / Q000528:radiation effects; D004269:DNA, Bacterial / Q000187:drug effects / Q000235:genetics* / Q000528:radiation effects; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism / Q000528:radiation effects; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D016685:Mitomycin; D008937:Mitomycins / Q000494:pharmacology; D009154:Mutation
D004269:DNA, Bacterial; D008937:Mitomycins; D016685:Mitomycin