Effect of 5-thio-D-glucose on testosterone biosynthesis in vivo in mice testes.
Testicular synthesis of (14C)cholesterol and (14C)testosterone from (14C)acetate were investigated in mice treated with 5-thio-D-glucose at a dose of 33 mg/kg body weight/day for 21 days. The testicular synthesis of free cholesterol as well as steroids were significantly decreased. The steroid synthesizing enzymes, cholesterol esterase, cholesterol side-chain cleaving enzyme, total alpha-hydroxysteroid dehydrogenase and total beta-hydroxysteroid dehydrogenase, were also analysed. Cholesterol esterase and total beta-hydroxysteroid dehydrogenase were significantly reduced whereas total alpha-hydroxysteroid dehydrogenase was unaffected. Hence, a decrease in free cholesterol for steroid synthesis and a decreased activity of the steroidogenic enzyme, beta-hydroxysteroid dehydrogenase, were responsible for the diminished synthesis of testosterone.
Veeraragavan
K
K
Ramakrishnan
S
S
D000085:Acetates / Q000378:metabolism; D019342:Acetic Acid; D000818:Animals; D002784:Cholesterol / Q000096:biosynthesis*; D002786:Cholesterol Side-Chain Cleavage Enzyme / Q000378:metabolism; D003687:Dehydroepiandrosterone / Q000096:biosynthesis; D005947:Glucose / Q000031:analogs & derivatives* / Q000494:pharmacology; D006913:Hydroxysteroid Dehydrogenases / Q000378:metabolism; D008297:Male; D051379:Mice; D011284:Pregnenolone / Q000096:biosynthesis; D011374:Progesterone / Q000096:biosynthesis; D002787:Sterol Esterase / Q000378:metabolism; D013737:Testis / Q000187:drug effects / Q000378:metabolism*; D013739:Testosterone / Q000096:biosynthesis*
D000085:Acetates; C003002:5-thio-D-glucose; D013739:Testosterone; D003687:Dehydroepiandrosterone; D011374:Progesterone; D011284:Pregnenolone; D002784:Cholesterol; D006913:Hydroxysteroid Dehydrogenases; D002786:Cholesterol Side-Chain Cleavage Enzyme; D002787:Sterol Esterase; D005947:Glucose; D019342:Acetic Acid