Identification of 19-hydroxy-progesterone in human placenta.
We have tentatively demonstrated the presence of a 19-hydroxylated C21 steroid, 19-hydroxy-progesterone, in normal human placenta. A 19-hydroxylated steroid such as 19-hydroxy-progesterone, if produced by the placenta, could serve as a precursor for such hypertensinogenic 19-nor-steroids as 19-nor-deoxycorticosterone and 19-nor-progesterone. Freshly delivered, homogenized placental tissue was extracted and subjected to thin layer chromatography. A steroid corresponding to standard 19-hydroxy-progesterone was subsequently purified in HPLC, where authentic 19-hydroxy-progesterone and the sample had the same retention time. The identity of the sample was further confirmed by repeat HPLC after acetylation and mass spectrometry. Our experiment indicates that 19-hydroxy-progesterone is present in term placental tissue, where it appears to be synthesized.
Melby
J C
JC
Evans Memorial Department of Clinical Research, University Hospital, Boston, MA 02118.
Holbrook
M
M
Ehlers
M E
ME
Kyei-Aboagye
K
K
Edelin
K
K
Bencsath
F A
FA
Field
F
F
Griffing
G T
GT
D000107:Acetylation; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D005260:Female; D006801:Humans; D006908:Hydroxyprogesterones / Q000032:analysis*; D013058:Mass Spectrometry; D010920:Placenta / Q000032:analysis*; D011247:Pregnancy
D006908:Hydroxyprogesterones; C057411:19-hydroxyprogesterone