title: Cell-autonomous action of the testis-determining gene: Sertoli cells are exclusively XY in XX----XY chimaeric mouse testes.
abstract: The distribution of XX and XY cells in XX----XY chimaeric mouse testes was analysed by enzyme marker analysis of separated testicular tissues and by in situ DNA marker analysis of air-dried testicular cells and testis sections. XX cells contributed to the Leydig cells, the peritubular cells and the vascularized connective tissue of the tunica albuginea. The Sertoli cells, on the other hand, appeared to be exclusively XY. These results indicate that during the development of the testis, Sertoli cell differentiation is triggered by cell-autonomous activity of the Y chromosomal testis-determining gene Tdy. Subsequent steps in testis differentiation may be a consequence of Sertoli cell activity.
authors.affiliation: MRC Mammalian Development Unit, London, UK.
authors.forename: P S
authors.initials: PS
authors.lastname: Burgoyne
authors.forename: M
authors.initials: M
authors.lastname: Buehr
authors.forename: P
authors.initials: P
authors.lastname: Koopman
authors.forename: J
authors.initials: J
authors.lastname: Rossant
authors.forename: A
authors.initials: A
authors.lastname: McLaren
mesh_terms: D000818:Animals; D002678:Chimera; D005796:Genes; D005819:Genetic Markers; D005956:Glucose-6-Phosphate Isomerase / Q000378:metabolism; D007985:Leydig Cells / Q000201:enzymology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA; D012708:Sertoli Cells / Q000032:analysis* / Q000201:enzymology; D013737:Testis / Q000196:embryology* / Q000201:enzymology; D014960:X Chromosome; D014998:Y Chromosome
chemical_list: D005819:Genetic Markers; D005956:Glucose-6-Phosphate Isomerase