Stimulation of hexose transport by human polymorphonuclear leukocytes: a possible role for protein kinase C.
The protein C kinase activators 1-O-oleoyl, 2-O-acetylglycerol, 12-O-tetradecanoyl phorbol-13-acetate, and mezerein, stimulated deoxyglucose uptake in human neutrophils. The responses were stimulus specific since no effect was noted with the diether analogues 1-O-hexadecyl-2-O-ethylglycerol, 1-O-palmitoyl-2-O-acetyl or 1-O-palmitoyl-3-O-acetyl diesters of propanediol, or with 1,2-diolein. Stimulation of deoxyglucose uptake had the characteristics of carrier facilitated hexose transport. Stimulated uptake of deoxy-glucose was inhibited by trifluoperazine (10-30 microM). Activation of protein kinase C therefore appears to trigger events involved in hexose transport.
McCall
C
C
Schmitt
J
J
Cousart
S
S
O'Flaherty
J
J
Bass
D
D
Wykle
R
R
D001693:Biological Transport, Active / Q000187:drug effects; D000001:Calcimycin / Q000494:pharmacology; D003571:Cytochalasin B / Q000494:pharmacology; D003847:Deoxyglucose / Q000378:metabolism; D004075:Diglycerides / Q000494:pharmacology; D004224:Diterpenes; D006601:Hexoses / Q000378:metabolism*; D006801:Humans; D007536:Isomerism; D009504:Neutrophils / Q000378:metabolism*; D011493:Protein Kinase C; D011494:Protein Kinases / Q000378:metabolism*; D013729:Terpenes / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014268:Trifluoperazine / Q000494:pharmacology
D004075:Diglycerides; D004224:Diterpenes; D006601:Hexoses; D013729:Terpenes; D014268:Trifluoperazine; C011309:mezerein; D000001:Calcimycin; D003571:Cytochalasin B; C038237:1-oleoyl-2-acetylglycerol; D003847:Deoxyglucose; D011494:Protein Kinases; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate