Endothelial cell proliferation in vitro after incubation with anticoagulant and antiplatelet drugs.
An in vitro tissue culture model was used in the evaluation of various anticoagulant and antiplatelet drugs on endothelial cell proliferation and eicosanoid production. Low concentrations, less than normally achieved plasma concentrations of various heparins (beef lung, porcine intestinal and low molecular weight heparin from porcine intestine), increased endothelial cell growth. Higher concentrations showed a decrease for regular heparins, most marked for beef lung heparin -45%, compared to -21% for porcine intestinal heparin, but still an increase, +22%, was noted for low molecular weight heparin. Of the antiplatelet drugs, ibuprofen and dazoxiben reduced plating efficiency and endothelial cell proliferation. Of the antiplatelet drugs, all but dipyridamole reduced prostacyclin metabolite production. The difference in endothelial cell proliferation between heparins is of great interest and warrants further investigation.
Lindblad
B
B
Department of Surgery, University of Michigan, Medical School, Ann Arbor.
Wright
S W
SW
Burkel
W E
WE
Wakefield
T W
TW
Graham
L M
LM
Sell
R
R
Stanley
J C
JC
D000818:Animals; D000925:Anticoagulants / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D003374:Coumarins / Q000494:pharmacology; D046508:Culture Techniques; D016861:Cyclooxygenase Inhibitors; D004176:Dipyridamole / Q000494:pharmacology; D004285:Dogs; D004730:Endothelium, Vascular / Q000166:cytology* / Q000187:drug effects; D006493:Heparin / Q000494:pharmacology; D008970:Molecular Weight; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D013930:Thromboxane-A Synthase / Q000037:antagonists & inhibitors
D000925:Anticoagulants; D003374:Coumarins; D016861:Cyclooxygenase Inhibitors; D010975:Platelet Aggregation Inhibitors; D004176:Dipyridamole; D006493:Heparin; C030123:coumarin; D013930:Thromboxane-A Synthase