Identification of HIV-specific oligoclonal immunoglobulins in serum of carriers of HIV antibody.
Zone electrophoresis on agarose gel was performed on serum samples from HIV-antibody carriers and negative controls. Nitrocellulose strips precoated with an HIV preparation were then placed on top of the gels and developed by an immunoblotting procedure. A positive reaction was demonstrated between the HIV antigens and the HIV-antibody-positive serum samples with hypergammaglobulinemia and oligoclonal IgG bands. A negative reaction was found between the HIV antigens and HIV-antibody-negative serum samples from a normal person and a patient with monoclonal gammopathy. The presence of oligoclonal IgG bands in the serum of HIV-antibody carriers, and their positive identification with HIV antigens, indicates a specific immune response of the host to the HIV infection and supports the use of oligoclonal IgG bands as markers to follow the course of HIV infection.
Papadopoulos
N M
NM
Clinical Chemistry Service, Clinical Center, NINCDS, Bethesda, MD 20892.
Costello
R
R
Ceroni
M
M
Moutsopoulos
H M
HM
D000914:Antibodies, Viral / Q000032:analysis*; D001797:Blood Protein Electrophoresis; D002353:Carrier State; D015483:HIV Antibodies; D006801:Humans; D007136:Immunoglobulins / Q000032:analysis* / Q000276:immunology
D000914:Antibodies, Viral; D015483:HIV Antibodies; D007136:Immunoglobulins