Age-related changes of long-chain fatty acid metabolism in rat liver.
Palmitate oxidation activity and the activities of several enzymes involved in long-chain fatty acid metabolism were examined in the liver of young adult (2-month-old) and senescent (32-month-old) female rats. Palmitate oxidation activity in rat liver mitochondria showed age-related decrease, as judged by the rates of both 14CO2 production and formation of radioactive acid-soluble products from [1-14C]palmitate. In addition, long-chain acyl-coenzyme A synthetase activity was found to be decreased in liver mitochondria and increased in liver microsomes in senescent rats. These results suggest that, in the rat liver, preferential channeling of long-chain fatty acids through the triacylglycerol synthetic pathway may increase with age, and as a result, energy production by their oxidation may decrease.
Mori
S
S
Second Department of Internal Medicine, School of Medicine, Chiba University, Japan.
Morisaki
N
N
Saito
Y
Y
Yoshida
S
S
D000375:Aging; D000818:Animals; D003066:Coenzyme A Ligases / Q000378:metabolism; D005231:Fatty Acids, Unsaturated / Q000378:metabolism*; D005260:Female; D008099:Liver / Q000378:metabolism*; D008930:Mitochondria, Liver / Q000201:enzymology; D010084:Oxidation-Reduction; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000378:metabolism; D051381:Rats
D005231:Fatty Acids, Unsaturated; D010169:Palmitic Acids; D019308:Palmitic Acid; D003066:Coenzyme A Ligases