Expression of heterologous genes in E. coli.
The strategies for the expression of cloned foreign genes in E. coli are described, and some of the tactics that can be employed to ensure high levels of expression are identified. More fundamental problems associated with scale-up, such as plasmid stability and protein processing, are also addressed.
Harris
T J
TJ
Division of Molecular Biology, Celltech Limited, Slough, Berkshire, UK.
Emtage
J S
JS
D000818:Animals; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D005796:Genes; D006801:Humans; D010957:Plasmids; D011499:Protein Processing, Post-Translational; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics*
D011994:Recombinant Proteins