Acid treatment of plasma for the inactivation of plasminogen activator inhibitor-1 (PAI-1).
The present study was initiated to assess the effectiveness of various acid treatments of blood or plasma in the inactivation of PAI-1. It was shown that a frequently used treatment of blood or plasma with 1 M acetate buffer, pH 3.9, only partially inactivated PAI-1. The inactivation of PAI-1 in plasma was found to depend upon pH and temperature, showing an optimal inactivation at a pH less than or equal to 3, at 37 degrees C.
de Maat
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MP
Dept. of Internal Medicine II, University Hospital Dijkzigt, Rotterdam, The Netherlands.
Kluft
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C
de Boer
K
K
Knot
E A
EA
Jie
A F
AF
D000085:Acetates; D019342:Acetic Acid; D006023:Glycoproteins / Q000037:antagonists & inhibitors* / Q000097:blood*; D006801:Humans; D006851:Hydrochloric Acid; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D010960:Plasminogen Activators / Q000037:antagonists & inhibitors*; D015849:Plasminogen Inactivators; D013696:Temperature; D010959:Tissue Plasminogen Activator / Q000097:blood
D000085:Acetates; D006023:Glycoproteins; D015849:Plasminogen Inactivators; D010960:Plasminogen Activators; D010959:Tissue Plasminogen Activator; D019342:Acetic Acid; D006851:Hydrochloric Acid