Molecular cloning and amino acid sequence of human enkephalinase (neutral endopeptidase).
We have isolated a cDNA clone encoding human enkephalinase (neutral endopeptidase, EC 3.4.24.11) in a lambda gt10 library from human placenta, and present the complete 742 amino acid sequence of human enkephalinase. The human enzyme displays a high homology with rat and rabbit enkephalinase. Like the rat and rabbit enzyme, human enkephalinase contains a single N-terminal transmembrane region and is likely to be inserted through cell membranes with the majority of protein, including its carboxy-terminus, located extracellularly.
Malfroy
B
B
Department of Pharmacological Sciences, Genentech, Inc., South San Francisco, CA 94080.
Kuang
W J
WJ
Seeburg
P H
PH
Mason
A J
AJ
Schofield
P R
PR
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002462:Cell Membrane / Q000201:enzymology; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis; D006801:Humans; D008666:Metalloendopeptidases / Q000032:analysis* / Q000235:genetics; D008969:Molecular Sequence Data; D015260:Neprilysin; D011817:Rabbits; D051381:Rats
D004247:DNA; D008666:Metalloendopeptidases; D015260:Neprilysin