title: Prostaglandin production by corpora lutea of rhesus monkeys: characterization of incubation conditions and examination of putative regulators.
abstract: Prostaglandins (PGs) are produced by the corpus luteum (CL) of many domestic and laboratory species and may play a role in CL regulation. The production of PGs by luteal tissue of the rhesus monkey has yet to be clearly elucidated. The production of PGE2, PGF2 alpha, and 6-keto-PGF1 alpha by CL from rhesus monkeys and the incubation conditions (time and cell number) that permit assessment of their synthesis were examined. CL (n = 3 per characterization) were surgically removed from nonpregnant monkeys during the mid-luteal phase of the menstrual cycle (approximately 8-10 days after ovulation). Luteal tissue was dissociated and the cells were incubated at varying concentrations for increasing periods of time at 37 degrees C. Subsequent to defining incubation conditions, various exogenous factors were examined for their potential to modify PG production. Indomethacin, calcium ionophore, human chorionic gonadotropin (hCG), estradiol-17 beta (E2), progesterone (P), testosterone (T), dihydrotestosterone (DHT), and 1-4-6 androstatriene-3, 17-dione (ATD) were incubated with luteal cells in increasing doses. PG and P concentrations in the medium were determined by radioimmunoassay. PGs in the medium after 6 h incubation were detectable at all cell concentrations tested (50,000, 100,000, 200,000 cells/tube). Concentrations of PGs and P increased with cell number (p less than 0.05). Luteal cells (50,000 cells/tube) were incubated for times of 0-24 h. Concentrations of P, PGE2, and PGF2 alpha in the medium were relatively low prior to incubation (0 h), increased (p less than 0.05) linearly within the first 6-12 h, and plateaued through the remaining 24 h.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Dairy Science, College of Agriculture, Ohio State University, Columbus 43210.
authors.forename: M S
authors.initials: MS
authors.lastname: Johnson
authors.forename: A C
authors.initials: AC
authors.lastname: Ottobre
authors.forename: J S
authors.initials: JS
authors.lastname: Ottobre
mesh_terms: D015121:6-Ketoprostaglandin F1 alpha / Q000096:biosynthesis; D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D003338:Corpus Luteum / Q000187:drug effects / Q000378:metabolism*; D015237:Dinoprost / Q000096:biosynthesis; D005260:Female; D007213:Indomethacin / Q000494:pharmacology; D008253:Macaca mulatta; D011374:Progesterone / Q000096:biosynthesis; D011453:Prostaglandins / Q000096:biosynthesis*; D011458:Prostaglandins E / Q000096:biosynthesis; D011863:Radioimmunoassay; D013256:Steroids / Q000494:pharmacology
chemical_list: D011453:Prostaglandins; D011458:Prostaglandins E; D013256:Steroids; D000001:Calcimycin; D011374:Progesterone; D015121:6-Ketoprostaglandin F1 alpha; D015237:Dinoprost; D007213:Indomethacin