Synthesis of a non-porous, polystyrene-based strong anion-exchange packing material and its application to fast high-performance liquid chromatography of proteins.
Adsorbed coating technology has been used to produce a strong anion-exchange stationary phase on 3-micron non-porous poly(styrene-divinylbenzene) particles. In order to take full advantage of the excellent kinetic properties of the resultant packing material, small columns of 5 mm X 6 mm I.D. were used. These columns were pressure- and pH-stable and allowed protein separations to be made in less than 1 min at ambient temperature.
Rounds
M A
MA
Department of Biochemistry, Purdue University, West Lafayette, IN 47907.
Regnier
F E
FE
D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation*; D002852:Chromatography, Ion Exchange; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D011137:Polystyrenes; D011312:Pressure; D011506:Proteins / Q000302:isolation & purification*
D007202:Indicators and Reagents; D011137:Polystyrenes; D011506:Proteins