Rapid scanning thermal lens/laser transmission densitometer.
An automated densitometer based on the thermal lens principle is described. The apparatus also operates as a conventional laser transmission densitometer. Comparison of the performance in both modes shows that thermal lens densitometry provides lower detection limits, but that transmission densitometry is more satisfactory at high optical densities. The instrument is characterized with proteins separated by SDS-PAGE and stained with Coomassie Brilliant Blue G250.
Peck
K
K
Department of Chemistry, University of Michigan, Ann Arbor 48109.
Demana
T
T
Morris
M D
MD
D003720:Densitometry / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D006358:Hot Temperature; D007834:Lasers; D011506:Proteins / Q000032:analysis; D013997:Time Factors
D011506:Proteins