Modifications of concanavalin A patterns of alpha 1-acid glycoprotein and alpha 2-HS glycoprotein in alcoholic liver disease.
In order to test whether abnormalities in hepatocytes affect the glycoprotein carbohydrate moiety, crossed immunoaffinoelectrophoresis (CIAE) with Concanavalin A (Con A) was used to study serum alpha 1-acid glycoprotein (alpha 1-AGP) and alpha 2-HS glycoprotein (alpha 2-HS) obtained from alcoholic patients with biopsy-proven liver disease. Cirrhotic patients, placed in groups C1, C2 or C3, according to Pugh's classification, were compared to healthy donors (N) and to steatosic non-cirrhotic patients (S). Con A CIAE patterns revealed in group N three subpopulations for alpha 2-HS and four for alpha 1-AGP. Two main results emerged from this study: (1) in the alcoholic groups, the proportions of Con A-unreactive subpopulations of both glycoproteins increased. Moreover, group N could be separated from group S and group S from all the cirrhotic groups. (2) There was a good correlation between the relative amounts in Con A-unreactive subpopulations of alpha 1-AGP and alpha 2-HS. The increases observed in Con A-unreactive subpopulations are probably a general phenomenon related to alterations in glycosylation processing during liver cell damage.
Jezequel
M
M
Laboratoire de Biochimie A, Hôpital Bichat, Paris, France.
Seta
N S
NS
Corbic
M M
MM
Feger
J M
JM
Durand
G M
GM
D000328:Adult; D000368:Aged; D001798:Blood Proteins / Q000032:analysis*; D003208:Concanavalin A; D005260:Female; D006801:Humans; D007123:Immunoelectrophoresis, Two-Dimensional / Q000379:methods; D008108:Liver Diseases, Alcoholic / Q000097:blood*; D008297:Male; D008875:Middle Aged; D009961:Orosomucoid / Q000032:analysis*; D060749:alpha-2-HS-Glycoprotein
C556354:AHSG protein, human; D001798:Blood Proteins; D009961:Orosomucoid; D060749:alpha-2-HS-Glycoprotein; D003208:Concanavalin A