[A quantitative measurement of the cerebral infarct focus induced by arachidonate infusion and the relationship between measured values and stroke signs].
A method for measuring the organic infarct focus induced by arachidonate infusion into rat brain was devised, and the statistical relationship between the measured values and stroke signs in the rat was studied. By the infusion of arachidonate, a high incidence of cerebral infarction was found in the live rats with uniformly necrotized foci. The size of these foci (infarction rate) were measured by transcripting them to a graduated brain sheet. The relationship between the independent parameter of the infarction rate and the dependent parameter of stroke signs was fully analyzed by multidimensional quantification. Many animals showed no stroke signs despite having lesions (false negative). By contract, no animal without any lesion showed stroke signs (false positive). When each parameter of these signs were quantified and normalized, the stumbling and abnormal posture signs showed a wide range of values, relatively accurately reflecting the infarction degree. Moreover, the highest partial correlation ratio between the various parameters was found to be that between the stumbling and abnormal posture stroke signs. Thus, it may be said that the stumbling and the abnormal posture stroke signs can be considered relatively good parameters for evaluating the degree of an infarction. These parameters should be useful for the testing of anti-infarction drugs.
Bando
K
K
Biological Section, Grelan Pharmaceutical Co., Ltd., Tokyo, Japan.
Hatanaka
Y
Y
Ohbayashi
S
S
Irino
O
O
D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids; D001921:Brain / Q000473:pathology*; D002544:Cerebral Infarction / Q000139:chemically induced / Q000188:drug therapy / Q000473:pathology*; D002561:Cerebrovascular Disorders; D004341:Drug Evaluation / Q000379:methods; D008297:Male; D010205:Pantothenic Acid / Q000031:analogs & derivatives / Q000627:therapeutic use; D010880:Piperidines / Q000627:therapeutic use; D051381:Rats; D011919:Rats, Inbred Strains; D005680:gamma-Aminobutyric Acid / Q000031:analogs & derivatives / Q000627:therapeutic use
D001095:Arachidonic Acids; D010880:Piperidines; C016030:pantogab; D010205:Pantothenic Acid; D016718:Arachidonic Acid; D005680:gamma-Aminobutyric Acid; C010739:ifenprodil