Quantification of the enantiomers of ofloxacin in biological fluids by high-performance liquid chromatography.
Two methods for the determination of (+)- and (-)-ofloxacin in biological fluids by high-performance liquid chromatography are described. The first method is separation on a chiral stationary phase with bovine serum albumin immobilized on silica gel. The second is the coupling of ofloxacin to L-leucinamide via diphenylphosphinyl chloride activation. The diastereoisomeric derivatives are then separated on a common reversed-phase column. The second method revealed only slight differences in the pharmacokinetics of (+)- and (-)-ofloxacin in humans after an intravenous administration of racemic ofloxacin.
Lehr
K H
KH
Hoechst AG, Frankfurt am Main, F.R.G.
Damm
P
P
D000890:Anti-Infective Agents / Q000032:analysis* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D006801:Humans; D007202:Indicators and Reagents; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D015242:Ofloxacin; D010078:Oxazines / Q000032:analysis* / Q000493:pharmacokinetics; D013237:Stereoisomerism
D000890:Anti-Infective Agents; D007202:Indicators and Reagents; D010078:Oxazines; D015242:Ofloxacin