Heparin-binding growth factor/prostatropin attenuates inhibition of rat prostate tumor epithelial cell growth by transforming growth factor type beta.
Normal rat prostate epithelial cell growth requires both epidermal growth factor and heparin-binding growth factor/prostatropin. In contrast, epithelial cells derived from the transplantable Dunning R3327H rat tumor require either epidermal growth factor or heparin-binding growth factor/prostatropin. Transforming growth factor type beta inhibited normal epithelial cell growth. Transforming growth factor beta inhibited epidermal growth factor-dependent growth of tumor epithelial cells, independent of epidermal growth factor concentrations. Transforming growth factor beta increased the effective dose of heparin-binding growth factor type 1 required to support tumor epithelial cell growth by 10-fold but saturating levels of heparin-binding growth factor type 1 (290 pM) completely attenuated the inhibitory effect of transforming growth factor beta. These results suggest that prostate tumor epithelial cells may escape the inhibitory effect of transforming growth factor beta as a consequence of alteration of the concurrent requirement for both epidermal growth factor (or homologues) and heparin-binding growth factors.
McKeehan
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W. Alton Jones Cell Science Center, Inc., Lake Placid, New York 12946.
Adams
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PS
D000818:Animals; D002452:Cell Count; D002455:Cell Division / Q000187:drug effects; D004848:Epithelium / Q000473:pathology; D016222:Fibroblast Growth Factor 2; D006133:Growth Substances / Q000494:pharmacology*; D006493:Heparin / Q000494:pharmacology*; D008297:Male; D008934:Mitogens; D009419:Nerve Tissue Proteins / Q000494:pharmacology*; D010455:Peptides / Q000494:pharmacology*; D011471:Prostatic Neoplasms / Q000473:pathology*; D051381:Rats; D015291:Transforming Growth Factors; D014407:Tumor Cells, Cultured
D006133:Growth Substances; D008934:Mitogens; D009419:Nerve Tissue Proteins; D010455:Peptides; D016222:Fibroblast Growth Factor 2; D015291:Transforming Growth Factors; D006493:Heparin