Construction of a bifunctional genetically labelled plasmid for Bacillus thuringiensis subsp. israelensis.
A small cryptic plasmid of Bacillus thuringiensis subsp. israelensis was labelled in vitro with two genetic markers. One of the recombinant plasmids was mapped and transformed in Escherichia coli, Bacillus subtilis and Bacillus thuringiensis. This and similar shuttle plasmids could be very useful as vectors for the investigation of the toxin genes in their own host.
Miteva
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VI
Department of Genetics, Bulgarian Academy of Sciences, Sofia.
Grigorova
R T
RT
D001412:Bacillus subtilis / Q000235:genetics; D001413:Bacillus thuringiensis / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005819:Genetic Markers; D005822:Genetic Vectors; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D015183:Restriction Mapping; D014169:Transformation, Bacterial
D004269:DNA, Bacterial; D005819:Genetic Markers