title: 500 MHz NMR characterization of synthetic bombesin and related peptides in DMSO-d6 by two-dimensional techniques.
abstract: The proton NMR characterization of bombesin has been carried out at 500 MHz in DMSO-d6 using two-dimensional homo- and 1H-13C hetero-correlated techniques. All resonances in the NMR spectra have been assigned and several coupling constants have been measured. The backbone J alpha CH-NH coupling constants have constant values that vary between 7.8 and 8.2 Hz and indicate an unfolded structure in DMSO-d6. Discrepancies with data recently obtained at 300 MHz [(1987) Eur. J. Biochem. 168, 193-199] are discussed.
authors.affiliation: Institute of Industrial Chemistry, University of Padua, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Di Bello
authors.forename: L
authors.initials: L
authors.lastname: Gozzini
authors.forename: M
authors.initials: M
authors.lastname: Tonellato
authors.forename: M
authors.initials: M
authors.lastname: Grazia Corradini
authors.forename: G
authors.initials: G
authors.lastname: D'Auria
authors.forename: L
authors.initials: L
authors.lastname: Paolillo
authors.forename: E
authors.initials: E
authors.lastname: Trivellone
mesh_terms: D000595:Amino Acid Sequence; D001839:Bombesin / Q000138:chemical synthesis; D003903:Deuterium; D004121:Dimethyl Sulfoxide; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D011487:Protein Conformation
chemical_list: D003903:Deuterium; D001839:Bombesin; D004121:Dimethyl Sulfoxide