title: Binding of the bovine basic pancreatic trypsin inhibitor (Kunitz) to human alpha-, beta- and gamma-thrombin; a kinetic and thermodynamic study.
abstract: Kinetic and thermodynamic parameters for the binding of the bovine basic pancreatic trypsin inhibitor (BPTI, Kunitz inhibitor) to human alpha-, beta- and gamma-thrombin have been determined, between 5 and 45 degrees C, at pH 7.5. BPTI-binding properties to human thrombins have been analyzed in parallel with those of serine (pro)enzymes acting on cationic and non-cationic substrates, with particular reference to the bovine beta-trypsin/BPTI system. The observed binding behaviour of BPTI to human alpha-, beta- and gamma-thrombin has been related to the inferred stereochemistry of the enzyme/inhibitor contact region(s).
authors.affiliation: C.N.R., Department of Biochemical Sciences, University of Rome, La Sapienza, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Ascenzi
authors.forename: M
authors.initials: M
authors.lastname: Coletta
authors.forename: G
authors.initials: G
authors.lastname: Amiconi
authors.forename: R
authors.initials: R
authors.lastname: de Cristofaro
authors.forename: M
authors.initials: M
authors.lastname: Bolognesi
authors.forename: M
authors.initials: M
authors.lastname: Guarneri
authors.forename: E
authors.initials: E
authors.lastname: Menegatti
mesh_terms: D000465:Algorithms; D000818:Animals; D002417:Cattle; D004151:Dipeptides / Q000378:metabolism; D007700:Kinetics; D013816:Thermodynamics; D013917:Thrombin / Q000378:metabolism*; D014360:Trypsin Inhibitor, Kunitz Soybean / Q000378:metabolism*; D014361:Trypsin Inhibitors / Q000378:metabolism*
chemical_list: D004151:Dipeptides; D014361:Trypsin Inhibitors; C021129:S 2238; D014360:Trypsin Inhibitor, Kunitz Soybean; D013917:Thrombin