Chloroacetaldehyde reacts with Z-DNA.
We show that chloroacetaldehyde, a chemical compound known to be reactive with unpaired adenine and cytosine residues, reacts with adenine residues (syn conformation) but not with cytosine residues (anti conformation) within Z-DNA. These modified residues are sensitive to cleavage by piperidine, which allows mapping at the single nucleotide level.
Vogt
N
N
Centre de Biophysique Moléculaire, Orléans, France.
Marrot
L
L
Rousseau
N
N
Malfoy
B
B
Leng
M
M
D000079:Acetaldehyde / Q000031:analogs & derivatives* / Q000378:metabolism; D001483:Base Sequence; D004247:DNA / Q000187:drug effects / Q000378:metabolism*; D009710:Nucleotide Mapping; D010880:Piperidines / Q000494:pharmacology
D010880:Piperidines; C032727:piperidine; D004247:DNA; C004656:chloroacetaldehyde; D000079:Acetaldehyde