The secretory effect of canatoxin on rat brain synaptosomes involves a lipoxygenase-mediated pathway.
Canatoxin (CNTX), a neurotoxic protein, is known to activate platelet secretion and aggregation in vitro through a lipoxygenase-dependent pathway. This study shows that CNTX also induces time and dose-dependent serotonin secretion from rat brain synaptosomes. The secretory effect induced by 6 microM CNTX was similar to that elicited by 150 mM KCl. Nordihydroguaiaretic acid (500 microM) completely abolished CNTX-induced serotonin release while 150 microM indomethacin had no effect. These data suggest the involvement of the lipoxygenase pathway in neurotransmitter release elicited by CNTX as occurs in the platelet.
Barja-Fidalgo
C
C
Departamento de Ciências Fisiológicas, Universidade do Estado do Rio de Janeiro, Brasil.
Guimarães
J A
JA
Carlini
C R
CR
D000818:Animals; D002396:Catechols / Q000494:pharmacology*; D004789:Enzyme Activation / Q000187:drug effects; D066298:In Vitro Techniques; D037102:Lectins / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D008084:Lipoxygenase / Q000378:metabolism; D009637:Masoprocol / Q000494:pharmacology*; D010940:Plant Proteins; D051381:Rats; D012701:Serotonin / Q000378:metabolism*; D013574:Synaptosomes / Q000187:drug effects / Q000378:metabolism*; D014118:Toxins, Biological
D002396:Catechols; D037102:Lectins; D010940:Plant Proteins; D014118:Toxins, Biological; D012701:Serotonin; D009637:Masoprocol; C032815:canatoxin; D008084:Lipoxygenase