Experimental laser phototherapy of the Morris 7777 hepatoma in the rat.
The tumoricidal effect of the activation of hematoporphyrin derivative (HpD), by an argon-ion-dye-laser (wavelength 630 nm), was investigated in the Buffalo rat bearing subcutaneous implants of the Morris 7777 hepatoma. Tumor growth was monitored by measuring the tumor volume with constant force calipers. In control animals and those that were pretreated with HpD alone (10 or 20 mg per kg by i.p. injection) or laser light alone (2,000 J at 100 mW), a predictable exponential growth pattern of the cancer was observed. Animals were pretreated with HpD (10 mg per kg by i.p. injection) 48 hr prior to the fiberoptic, intratumor delivery of laser radiation (2,000 J at 100 mW), when the tumor had reached a volume greater than 1.5 cm3. Forty-eight hours after combined laser and HpD treatment, the hepatoma underwent coagulation necrosis, and the tumor volume rapidly increased from a mean value of 1.8 +/- 0.7 cm3 S.D. to a value of 5.8 +/- 1.5 cm3 S.D., compared with a mean of 3.7 +/- 0.7 cm3 S.D. in animals who had not received laser phototherapy. Rats treated with HpD and laser light survived longer (mean survival time 48 +/- 12 days S.D.) than did the other animals treated with the laser alone or HpD alone (mean survival time 31 +/- 16.5 S.D.). Tissue biodistribution studies with tritiated hematoporphyrin derivative, given with doses of 10 and 20 mg per kg of HpD, showed higher concentrations of the dye in the liver, kidneys and spleen than in the tumor at 48 hr after administration of the dye.(ABSTRACT TRUNCATED AT 250 WORDS)
Holt
S
S
Tulip
J
J
Hamilton
D
D
Cummins
J
J
Fields
A
A
Dick
C
C
D000818:Animals; D017324:Hematoporphyrin Derivative; D006415:Hematoporphyrins / Q000378:metabolism / Q000627:therapeutic use*; D053685:Laser Therapy; D008114:Liver Neoplasms, Experimental / Q000188:drug therapy* / Q000378:metabolism / Q000648:ultrastructure; D009368:Neoplasm Transplantation; D010778:Photochemotherapy; D011838:Radiation-Sensitizing Agents / Q000378:metabolism / Q000627:therapeutic use*; D051381:Rats; D014018:Tissue Distribution
D006415:Hematoporphyrins; D011838:Radiation-Sensitizing Agents; D017324:Hematoporphyrin Derivative