Mouse peritoneal macrophages plated on mannan- and horseradish peroxidase-coated substrates lose the ability to phagocytose by their Fc receptors.
Ligand-conjugated substrates were used to study mouse macrophage receptor-ligand interactions. Both resident and thioglycollate-elicited macrophages plated on substrates conjugated with mannans and horseradish peroxidase (HRP), ligands for the Man/GlcNAc receptor, lost their ability to phagocytose zymosan. In addition, these macrophages also lost their ability to phagocytose IgG-coated erythrocytes (E(IgG] via their Fc receptors (FcR). The abrogation of Fc receptor-mediated phagocytosis occurred as early as 4 hr after macrophage plating on HRP-coated substrates and was dependent on the amount of HRP conjugated to the substrate. Macrophages plated on those substrates showed a 70% reduction in E(IgG) binding and the same decrease (approximately 35%) in binding of 125I-labeled Fab fragment of monoclonal anti-IgG2b FcR antibody as macrophages plated on dinitrophenyl-anti-dinitrophenyl IgG immune complexes. We interpret these results as showing that modulation of macrophage mannose/GlcNAc receptors induces modulation of FcR.
Sung
S S
SS
Nelson
R S
RS
Silverstein
S C
SC
D000818:Animals; D003470:Culture Media; D006735:Horseradish Peroxidase / Q000378:metabolism / Q000494:pharmacology*; D007108:Immune Tolerance; D007074:Immunoglobulin G / Q000502:physiology; D008264:Macrophages / Q000378:metabolism* / Q000502:physiology; D008351:Mannans / Q000378:metabolism / Q000494:pharmacology*; D037241:Mannose-Binding Lectins; D051379:Mice; D010544:Peroxidases / Q000494:pharmacology*; D010587:Phagocytosis / Q000187:drug effects*; D010873:Pinocytosis / Q000187:drug effects; D011961:Receptors, Fc / Q000187:drug effects / Q000276:immunology / Q000502:physiology*; D017452:Receptors, IgG; D011971:Receptors, Immunologic / Q000187:drug effects / Q000502:physiology*; D015054:Zymosan
D003470:Culture Media; D007074:Immunoglobulin G; D008351:Mannans; D037241:Mannose-Binding Lectins; D011961:Receptors, Fc; D017452:Receptors, IgG; D011971:Receptors, Immunologic; C035313:mannose-N-acetylglucosamine receptor; D015054:Zymosan; D006735:Horseradish Peroxidase; D010544:Peroxidases