title: Oestrogen receptor activation and molecular forms in human breast cancer.
abstract: The oestrogen receptor (ER) present in human breast cancer cytosol was resolved by high performance size exclusion chromatography into a high molecular weight form (300 kDa) either alone or together with a lower molecular weight species (60 kDa). In tumours that contained the 60 kDa species there was a significant increase both in ER activation, determined by binding to oligo(dT)-cellulose, and the concentration of progesterone receptor (PR). Despite cytosolic ER being a potential homogenisation artefact, qualitative differences observed in vitro may reflect aspects of tumour biology in vivo.
authors.affiliation: Institute of Obstetrics and Gynaecology, Royal Postgraduate Medical School, Hammersmith Hospital, London, England.
authors.forename: G
authors.initials: G
authors.lastname: Parmar
authors.forename: M G
authors.initials: MG
authors.lastname: Elder
authors.forename: J O
authors.initials: JO
authors.lastname: White
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology; D001943:Breast Neoplasms / Q000378:metabolism*; D002482:Cellulose / Q000031:analogs & derivatives / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D003600:Cytosol / Q000378:metabolism; D006358:Hot Temperature; D006801:Humans; D008970:Molecular Weight; D008982:Molybdenum / Q000494:pharmacology; D009838:Oligodeoxyribonucleotides / Q000378:metabolism; D011960:Receptors, Estrogen / Q000187:drug effects / Q000378:metabolism*; D011980:Receptors, Progesterone / Q000378:metabolism
chemical_list: D009838:Oligodeoxyribonucleotides; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone; C021722:oligo(dT)-cellulose; D008982:Molybdenum; D000255:Adenosine Triphosphate; D002482:Cellulose; C024687:sodium molybdate(VI)