Use of lacZ expression to monitor transcription.
For use in monitoring transcription in operon fusion, we have constructed a lacZ sequence with the initiation codon ATG and the Escherichia coli consensus Shine-Dalgarno site. There are unique restriction endonuclease sites flanking the sequence to allow easy isolation of the lacZ sequence with or without the Shine-Dalgarno site. We have placed this lacZ sequence behind the bovine growth hormone (BGH) gene and found that the lacZ product beta-galactosidase synthesized reflects the level of BGH-specific mRNA.
Tomich
C S
CS
Molecular Biology Research, Upjohn Company, Kalamazoo, Michigan 49007.
Kaytes
P S
PS
Olsen
M K
MK
Patel
H
H
D000818:Animals; D002417:Cattle; D003001:Cloning, Molecular; D003062:Codon; D004926:Escherichia coli / Q000235:genetics*; D005796:Genes; D005798:Genes, Bacterial; D013006:Growth Hormone / Q000235:genetics; D007763:Lac Operon; D015345:Oligonucleotide Probes; D010957:Plasmids; D015183:Restriction Mapping; D014158:Transcription, Genetic; D001616:beta-Galactosidase / Q000235:genetics
D003062:Codon; D015345:Oligonucleotide Probes; D013006:Growth Hormone; D001616:beta-Galactosidase