Differentiation of erythroid progenitor (CFU-E) cells from mouse fetal liver cells and murine erythroleukemia (TSA8) cells without proliferation.
Erythropoietin (epo) appears to play a significant role in influencing the proliferation and differentiation of erythroid progenitor (CFU-E) cells. To determine the mechanism of action of epo, the effect of drugs on the in vitro colony formation of CFU-E cells induced from a novel murine erythroleukemia cell line, TSA8, was examined. While cytosine arabinoside inhibited colony formation and terminal differentiation of the CFU-E cells responding to epo, herbimycin, which is a drug that inhibits src-related phosphorylation, inhibited colony formation only. The same effect of herbimycin was observed with normal CFU-E cells from mouse fetal liver cells. These results suggest that epo induces two signals, one for proliferation and the other for differentiation, and that the two signals are not linked in erythroid progenitor cells.
Noguchi
T
T
Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
Fukumoto
H
H
Mishina
Y
Y
Obinata
M
M
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D016227:Benzoquinones; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004921:Erythropoietin / Q000494:pharmacology*; D006412:Hematopoietic Stem Cells / Q000187:drug effects*; D047029:Lactams, Macrocyclic; D004915:Leukemia, Erythroblastic, Acute / Q000503:physiopathology*; D008099:Liver / Q000166:cytology / Q000196:embryology / Q000502:physiology*; D051379:Mice; D011809:Quinones / Q000494:pharmacology; D017828:Rifabutin / Q000031:analogs & derivatives
D000900:Anti-Bacterial Agents; D016227:Benzoquinones; D047029:Lactams, Macrocyclic; D011809:Quinones; D004921:Erythropoietin; D017828:Rifabutin; C020754:herbimycin