[Characteristics of the effect of Ca/Mg-dependent endonuclease from cell nuclei of human lymphocytes on J kappa-cluster of immunoglobulin genes].
The cleavage of the murine J kappa-segments by purified Ca/Mg-dependent endonuclease was studied. The enzyme was shown to cleave specifically the J kappa-segments introducing the double strand breaks in 5'-regions of the J kappa-genes. The number of thus generated discreet fragments does not change during incubation. Specificity of the enzyme is not changed by substitution of Ca/Mg for Mn. The enzyme activity is inhibited by 0.1 M NaCl as well as its specificity. Comparison of the enzyme with the known nuclease activities that cleave specifically the J kappa-segments demonstrated that the activities of the latter are determined by Ca/Mg-dependent endonuclease. The results suggest the possible participation of Ca/Mg-dependent endonuclease in Ig-genes recombination.
Morozov
A Iu
AIu
Urakov
D N
DN
Khodarev
N N
NN
Deev
S M
SM
Polianovskiĭ
O L
OL
D000818:Animals; D002467:Cell Nucleus / Q000201:enzymology; D004706:Endodeoxyribonucleases / Q000235:genetics* / Q000378:metabolism; D005803:Genes, Immunoglobulin; D006801:Humans; D006868:Hydrolysis; D007145:Immunoglobulin kappa-Chains / Q000235:genetics*; D008214:Lymphocytes / Q000201:enzymology*; D051379:Mice; D005810:Multigene Family
D007145:Immunoglobulin kappa-Chains; D004706:Endodeoxyribonucleases; C029367:calcium magnesium dependent endodeoxyribonuclease
Kharakteristika deĭstviia Ca/Mg-zavisimoĭ éndonukleazy kletochnykh iader limfotsitov cheloveka na J kappa-klaster immunoglobulinovykh genov.