[Role of lipid peroxidation in changes in the structure of Ca-ATPase in skeletal muscle sarcoplasmic reticulum during hypercholesterolemia].
Using the method of spin labels it was shown that in hypercholesterolemia (HCh), the following parameters decreased: the velocity of maleimide spin label binding to sarcoplasmic reticulum (SR) Ca-ATPase of rabbit skeletal muscles, the accessibility of spin-labeled thiol groups of the enzyme to potassium ferricyanide and sodium ascorbate, and the mobility of the Ca-ATPase molecule fragment to which the spin label was attached. In addition, intensification of lipid peroxidation was demonstrated in SR membranes. Supplementation of the high-cholesterol diet with alpha-tocopherol resulted in the decreased rates of lipid peroxidation in SR membranes and increased values of the above parameters relative to the values found under HCh. It is concluded that the effect of alpha-tocopherol in vivo on the structure of the Ca-ATPase proteolipid complex in HCh is due mainly to antioxidant properties of the diet-supplementing substance.
Timofeev
A A
AA
Azizova
O A
OA
Chernysheva
G V
GV
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D000252:Calcium-Transporting ATPases / Q000378:metabolism*; D006937:Hypercholesterolemia / Q000378:metabolism*; D008054:Lipid Peroxides / Q000378:metabolism*; D008297:Male; D011817:Rabbits; D012519:Sarcoplasmic Reticulum / Q000201:enzymology*; D024505:Tocopherols; D014810:Vitamin E / Q000031:analogs & derivatives / Q000494:pharmacology; D024502:alpha-Tocopherol / Q000031:analogs & derivatives*
D000975:Antioxidants; D008054:Lipid Peroxides; D014810:Vitamin E; D000252:Calcium-Transporting ATPases; D024502:alpha-Tocopherol; D024505:Tocopherols
Rol' perekisnogo okisleniia lipidov v izmenenii struktury Ca-ATFazy sarkoplazkaticheskogo retikuluma skeletnykh myshts pri giperkholesterinemii.