Structure of the O-chain of the lipopolysaccharide of Pasteurella haemolytica serotype T3.
Cell-wall lipopolysaccharide isolated from Pasteurella haemolytica serotype T3 using the phenol-water extraction procedure was shown to be an S type lipopolysaccharide by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Hydrolysis with mild acid afforded a lipid-free, antigenic O-chain polysaccharide. On the basis of one- and two-dimensional 1H and 13C nuclear magnetic resonance studies, in conjunction with microanalytical chemical methods, the O-polysaccharide was determined to be a linear polymer of a disaccharide repeating unit having the structure. [----3)-beta-D-G1cpNAc-(1----4)-alpha-L-Rhap-(1----]n
Leitch
R A
RA
Division of Biological Sciences, National Research Council of Canada, Ottawa, Ont.
Richards
J C
JC
D002240:Carbohydrate Sequence; D055598:Chemical Phenomena; D002621:Chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D005779:Immunodiffusion; D008070:Lipopolysaccharides / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D010325:Pasteurella / Q000276:immunology*
D008070:Lipopolysaccharides