The elastogenic effect of recombinant transforming growth factor-beta on porcine aortic smooth muscle cells.
Transforming growth factor-beta, a peptide growth factor, is known to be a multifunctional regulator of cellular activity. The effect of this growth factor on extracellular matrix formation is well established, but its effects on elastin, a critical component of lung, skin, and blood vessels are unknown. In the present study, by use of an Enzyme-Linked Immunoassay method, we found that transforming growth factor-beta strongly increased elastin production in cultured porcine aortic smooth muscle cells. In a dosage-dependent study, 1.0-10.0 ng/ml transforming growth factor-beta promoted elastin production 2-3 fold. In a time-dependent study, at least an 8 h pre-treatment with 10.0 ng/ml transforming growth factor-beta was required for sustained increases in elastin production. The effects of transforming growth factor-beta on cultured aortic smooth muscle cells suggest that this cytokine may be an important mediator of elastin formation during atherosclerosis and hypertension.
Liu
J M
JM
Department of Pathology, Vanderbilt University School of Medicine, Nashville, Tennessee.
Davidson
J M
JM
D000818:Animals; D001011:Aorta / Q000187:drug effects / Q000378:metabolism*; D002478:Cells, Cultured; D004549:Elastin / Q000096:biosynthesis*; D004797:Enzyme-Linked Immunosorbent Assay; D006133:Growth Substances / Q000494:pharmacology*; D006801:Humans; D007700:Kinetics; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000378:metabolism*; D010455:Peptides / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000494:pharmacology*; D013552:Swine; D015291:Transforming Growth Factors
D006133:Growth Substances; D010455:Peptides; D011994:Recombinant Proteins; D015291:Transforming Growth Factors; D004549:Elastin