Immunophenotype of lymphoid cells in positive patch tests of allergic contact dermatitis.
The immune phenotype of the infiltrating cells in 13 positive patch tests from 8 cases of contact dermatitis and 1 case of poison ivy was studied. An indirect immunoperoxidase technique was used in conjunction with monoclonal antibodies directed against mature T cells (Leu-1, T11), helper T cells (Leu-3A), suppressor/cytotoxic T cells (Leu-2A), killer and natural killer cells (HNK 1), B cells (B1), Langerhans cells (HLA-DR), and the common acute lymphoblastic leukemia antigen (CALLA), (J5). The majority of infiltrating mononuclear cells were Leu-1+, T11+, Leu-3A+, Leu-2A-, HLA-DR+, T9-, T10-, HNK-, B1-, J5-. Occasional T6+ cells were observed in the epidermis (including spongiotic microvesicles) and also isolated in the dermis and within the dermal mononuclear infiltrates. The phenotype was compared with cutaneous T-cell lymphoma, a disease in which contact allergy and antigenic persistence may play a role.
McMillan
E M
EM
Stoneking
L
L
Burdick
S
S
Cowan
I
I
Husain-Hamzavi
S L
SL
D000911:Antibodies, Monoclonal / Q000276:immunology; D003877:Dermatitis, Contact / Q000276:immunology* / Q000473:pathology; D011040:Dermatitis, Toxicodendron / Q000276:immunology; D000078404:Epidermal Cells; D004817:Epidermis / Q000276:immunology; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques; D008214:Lymphocytes / Q000145:classification / Q000276:immunology*; D010328:Patch Tests; D010641:Phenotype; D012867:Skin / Q000166:cytology / Q000276:immunology
D000911:Antibodies, Monoclonal; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II