Structural determination of the photosystem II core complex from spinach.
A photosystem II core complex was purified with high yield from spinach by solubilization with beta-dodecylmaltoside. The complex consisted of polypeptides with molecular mass 47, 43, 34, 31, 9 and 4 kDa and some minor components, as detected by silver-staining of polyacrylamide gels. There was no indication for the chlorophyll-a/b-binding, light-harvesting complex polypeptides. The core complex revealed electron-transfer activity (1,5-diphenylcarbazide----2,6-dichloroindophenol) of about 30 mumol reduced 2,6-dichloroindophenol/mg chlorophyll/h. The structural integrity was analyzed by electron microscopy. The detergent-solubilized protein complex has the shape of a triangular disk with a maximum diameter of 13 nm and a maximum height of 6.8 nm. The shape of this core complex differs considerably from that of cyanobacterial photosystem II membrane fragments, which are elongated particles. The structural differences between both the complexes of higher plants and cyanobacteria are discussed with special emphasis on their association with the antenna apparatus in the photosynthetic membranes.
Irrgang
K D
KD
Max-Volmer-Institut für Biophysikalische und Physikalische Chemie, Technische Universität Berlin.
Boekema
E J
EJ
Vater
J
J
Renger
G
G
D001426:Bacterial Proteins / Q000302:isolation & purification; D002499:Centrifugation, Density Gradient; D002734:Chlorophyll / Q000032:analysis / Q000302:isolation & purification*; D000458:Cyanobacteria / Q000032:analysis; D004583:Electrons; D004591:Electrophoresis, Polyacrylamide Gel; D004735:Energy Transfer; D045342:Light-Harvesting Protein Complexes; D008854:Microscopy, Electron; D010455:Peptides / Q000302:isolation & purification; D045322:Photosynthetic Reaction Center Complex Proteins; D045332:Photosystem II Protein Complex; D010940:Plant Proteins / Q000302:isolation & purification*; D010945:Plants, Edible / Q000032:analysis*; D012995:Solubility
D001426:Bacterial Proteins; D045342:Light-Harvesting Protein Complexes; D010455:Peptides; D045322:Photosynthetic Reaction Center Complex Proteins; D045332:Photosystem II Protein Complex; D010940:Plant Proteins; D002734:Chlorophyll