Trypanosoma brucei brucei: variability in the association of some variant surface glycoproteins.
In our isolation procedure, the surface antigens of the variants AnTat 1.1 and 1.10 (Trypanosoma brucei brucei) are essentially obtained as a disulfide-linked dimer while the AnTat 1.8 surface antigen is found as a mixture of monomer and disulfide-linked dimer. This observation may be related to the localization of the cysteine residues in the protein sequences. In the purification procedure using concanavalin-A Sepharose chromatography, besides the VSG elution by methyl-alpha-D-mannopyranoside, a quantitative elution of still bound VSG may be obtained by the addition of beta-mercaptoethanol to methyl-alpha-D-mannopyrannoside in the elution buffer. The surface antigen of the variant AnTat 1.1 was examined for molecular form at several different times during the release procedure. The disulfide-linked dimer could be observed within 30 min of the surface coat release, indicating its presence within the parasite.
Huet-Duvillier
G
G
Unité INSERM No. 16, Lille, France.
Gomes
V
V
Tetaert
D
D
Mathon
P
P
Boersma
A
A
Degand
P
P
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004220:Disulfides / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D051381:Rats; D014346:Trypanosoma brucei brucei / Q000032:analysis*; D014643:Variant Surface Glycoproteins, Trypanosoma / Q000032:analysis*
D004220:Disulfides; D014643:Variant Surface Glycoproteins, Trypanosoma