Stimulation of rabbit synoviocyte prostaglandin E2 synthesis by lipopolysaccharides and their subunit structures.
Lipopolysaccharides (LPS) induce synoviocyte activation and may lead to destruction of synovial joint tissues. We assessed the production of prostaglandin E2 (PGE2) as a measure of synoviocyte activation by LPS and their subunit structures. Diphosphoryl lipid A was the smallest portion of lipid A tested that stimulated PGE2 production. The polysaccharide fraction of LPS, containing the O antigen, was also active. Intraarticular injections of the polysaccharide resulted in a synovitis very similar to that found in association with the intact LPS molecule. These observations suggest that both parts of LPS might be involved in gram-negative, organism-associated synovitis.
Rothenberg
R J
RJ
Department of Pathology, William S. Middleton Memorial Veterans Hospital, Madison, Wisconsin 53705.
England
D
D
Qureshi
N
N
Takayama
K
K
D000818:Animals; D002478:Cells, Cultured; D015232:Dinoprostone; D004305:Dose-Response Relationship, Drug; D007719:Knee Joint / Q000473:pathology; D008070:Lipopolysaccharides / Q000008:administration & dosage / Q000494:pharmacology*; D011134:Polysaccharides / Q000502:physiology; D011458:Prostaglandins E / Q000096:biosynthesis*; D011817:Rabbits; D013582:Synovial Fluid / Q000166:cytology*; D013583:Synovial Membrane / Q000473:pathology
D008070:Lipopolysaccharides; D011134:Polysaccharides; D011458:Prostaglandins E; D015232:Dinoprostone