Postnatal development of beta-endorphin immunoreactivity in the medulla oblongata of rat.
Beta-endorphin (B-END) like immunoreactivity (i.r.) levels were measured by radioimmunoassay in the medulla oblongata of developing rats on postnatal ages P1-P42 at 7 day intervals, and in adult rats. From P1 to P42, B-END i.r. increased from 77.0 +/- 1.3 fm to 900.0 +/- 21.6 fm per medulla region (Mean +/- S.E.M.). Adult levels of B-END i.r. were 852.0 +/- 17.0 fm per medulla region. When B-END i.r. was determined per unit protein during this developmental period, a statistically significant change in levels was noted. B-END i.r. dropped from P1 to P7, and then increased from P7 to P14 (P less than 0.01). From P14 through adult, levels did not change significantly. Despite a "drop-out" in the observed immunostaining of B-END neurons in caudal medulla (perikarya in the nucleus tractus solitarius) at P21, radioimmunoassayable levels of this peptide remained constant from P21 through adult per unit protein.
Alessi
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Khachaturian
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H
D000818:Animals; D004723:Endorphins / Q000032:analysis / Q000378:metabolism*; D007124:Immunoenzyme Techniques; D008526:Medulla Oblongata / Q000032:analysis / Q000254:growth & development / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000032:analysis; D011863:Radioimmunoassay; D051381:Rats; D001615:beta-Endorphin
D004723:Endorphins; D009419:Nerve Tissue Proteins; D001615:beta-Endorphin