Enhancing effect of IFN-gamma on helper T cell activity and IL 2 production.
A single injection of young murine immune interferon (IFN-gamma) in young (3 mo) or old (14 to 24 mo) mice 3 days before carrier-priming significantly enhances helper T cell activity of their spleen cells. Maximal enhancement is attained when IFN-gamma is injected once immediately before priming or for 4 consecutive days from the time of priming. Helper activity for anti-TNP antibody response was titrated in vitro by adding graded numbers of spleen cells from HRBC-primed mice of a given age to cultures containing a constant number of spleen cells from 3-mo-old normal mice and TNP-HRBC. When T cell-enriched spleen cells from HRBC-primed young or old mice, uninjected or injected with IFN-gamma, were separated by nylon wool filtration into passed (Thi) and adherent (Th2) cells, the helper activity of both T cell subpopulations was found to be enhanced by IFN-gamma injection. Helper activity of purified Th1 and Th2 cells was also increased by their in vitro preincubation with IFN-gamma. Furthermore, interleukin 2 (IL 2) production by mitogen-activated spleen cells from young and old mice is enhanced by addition of IFN-gamma to cultures. These data altogether indicate that IFN-gamma plays an important role in immunoregulation of helper T cell activity.
Frasca
D
D
Adorini
L
L
Landolfo
S
S
Doria
G
G
D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000494:pharmacology*; D000375:Aging; D000818:Animals; D004306:Dose-Response Relationship, Immunologic; D006863:Hydrogen-Ion Concentration; D007275:Injections, Intravenous; D007371:Interferon-gamma / Q000008:administration & dosage / Q000235:genetics / Q000494:pharmacology*; D007376:Interleukin-2 / Q000096:biosynthesis*; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D013154:Spleen / Q000166:cytology / Q000378:metabolism; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology* / Q000502:physiology
D000276:Adjuvants, Immunologic; D007376:Interleukin-2; D007371:Interferon-gamma