Photoprotective effect of vitamins A and E on polyamine and oxygenated free radical metabolism in hairless mouse epidermis.
The purpose of this study was to confirm the photoprotective effect on skin of vitamins A and E, due to inhibition of polyamine synthesis and production of free radicals. These variables were measured in the lumbar epidermis of the female hairless mouse subjected to UVA + B irradiation. Polyamines were assayed in epidermal homogenate by HPLC, and production of oxygenated free radicals was determined by spectrofluorometric assay of malonyl dialdehyde. It was determined that butyl-hydroxy-toluene and vitamin E inhibited production of free radicals (56% and 60%, respectively) and caused a significant reduction in polyamine biosynthesis (P less than 0.01), whereas the inhibitory effect of malonyl dialdehyde induced by vitamin A (30%) had no associated effect on polyamine metabolism.
Khettab
N
N
Laboratoire de Biochimie et de Physiologie, Faculté de Pharmacie, Nantes, France.
Amory
M C
MC
Briand
G
G
Bousquet
B
B
Combre
A
A
Forlot
P
P
Barey
M
M
D000818:Animals; D002084:Butylated Hydroxytoluene / Q000008:administration & dosage / Q000494:pharmacology; D002338:Carotenoids / Q000008:administration & dosage / Q000494:pharmacology; D004817:Epidermis / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D005260:Female; D005609:Free Radicals; D006801:Humans; D008315:Malondialdehyde / Q000096:biosynthesis; D051379:Mice; D008812:Mice, Hairless; D010100:Oxygen / Q000378:metabolism*; D011073:Polyamines / Q000096:biosynthesis*; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D014466:Ultraviolet Rays; D014801:Vitamin A / Q000494:pharmacology*; D014810:Vitamin E / Q000494:pharmacology*; D019207:beta Carotene
D005609:Free Radicals; D011073:Polyamines; D011837:Radiation-Protective Agents; D019207:beta Carotene; D014801:Vitamin A; D014810:Vitamin E; D002084:Butylated Hydroxytoluene; D002338:Carotenoids; D008315:Malondialdehyde; D010100:Oxygen