Protein kinase C activators mimic the M2-muscarinic receptor-mediated effects on the action potential in isolated sympathetic neurons of rabbits.
Protein kinase C activators 1,2-oleoylacetylglycerol (OAG, 0.5-50 microM), a synthetic diacylglycerol analog, and phorbol-12,13-dibutyrate (Pb(Bu)2, 0.016-1.6 microM) depressed the calcium (Ca)-dependent components of action potentials in isolated superior cervical ganglion cells of rabbits. Similar depressions were elicited when the M2-muscarinic receptors were activated. This muscarinic modification of the action potential was obscured after the perfusion with protein kinase C inhibitor 1-(5-isoquinolinesulfonyl)-2-methylpiperazine (H-7, 50 microM). It seems that protein kinase C is an intermediator between the M2-muscarinic receptors and the Ca channels regulating the firing rate of the postganglionic cells.
Mochida
S
S
Department of Physiology, Tokyo Medical College, Japan.
Kobayashi
H
H
D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D000200:Action Potentials / Q000187:drug effects; D000320:Adrenergic Fibers / Q000187:drug effects / Q000502:physiology*; D000818:Animals; D002118:Calcium / Q000502:physiology; D004075:Diglycerides / Q000494:pharmacology*; D005989:Glycerides / Q000494:pharmacology*; D066298:In Vitro Techniques; D007546:Isoquinolines / Q000494:pharmacology; D015240:Phorbol 12,13-Dibutyrate; D010703:Phorbol Esters / Q000494:pharmacology*; D010879:Piperazines / Q000494:pharmacology; D011493:Protein Kinase C / Q000378:metabolism / Q000502:physiology*; D011817:Rabbits; D011976:Receptors, Muscarinic / Q000502:physiology*; D013779:Tetrodotoxin / Q000494:pharmacology
D004075:Diglycerides; D005989:Glycerides; D007546:Isoquinolines; D010703:Phorbol Esters; D010879:Piperazines; D011976:Receptors, Muscarinic; D015240:Phorbol 12,13-Dibutyrate; D013779:Tetrodotoxin; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; C038237:1-oleoyl-2-acetylglycerol; D011493:Protein Kinase C; D002118:Calcium