Recombinant interleukin 2 differentiates alloantigen-primed Lyt-2+ T cells into the activated cytotoxic state.
The possibility of the generation of secondary cytotoxic T lymphocyte (2 degrees CTL) activity from alloantigen primed T cells by genetically homogeneous recombinant human interleukin 2(G-IL2) was analyzed. Not only purified IL2 by cell culture (C-IL2) but also G-IL2 induced 2 degrees CTL activity from primed T cells generated in mixed lymphocyte culture. This induction process required RNA and protein synthesis, while DNA synthesis was not relevant. In parallel to the induction of 2 degrees CTL activity, transition of the primed cells from the resting G1a to activated G1b has taken place. 2 degrees CTL activity was induced from nylon column-purified primed T cells in the absence of accessory cells and also in the absence of Lyt-1+ T cells. Interferon-gamma (IFN-gamma) induction was detected during the course of activation of alloantigen-primed T cells into the cytotoxic state. When IL2 was absorbed, IFN-gamma in the culture supernatant did not induce 2 degrees CTL activity. These results suggest that the IL2 molecule possessing T cell growth factor properties shares the nature of differentiation factor in terms of the activation of alloantigen-primed T cells into cytoxic state. The role of IFN-gamma produced in situ for this activation process awaits further investigation.
Yoshimoto
R
R
Kashima
N
N
Okada
K
K
Amikura
K
K
Hamuro
J
J
D000818:Animals; D000950:Antigens, Ly / Q000276:immunology*; D002453:Cell Cycle; D003001:Cloning, Molecular; D005260:Female; D007156:Immunologic Memory; D066298:In Vitro Techniques; D007376:Interleukin-2 / Q000235:genetics / Q000276:immunology*; D008211:Lymphocyte Cooperation; D007959:Lymphocyte Culture Test, Mixed; D051379:Mice; D008815:Mice, Inbred Strains; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D000950:Antigens, Ly; D007376:Interleukin-2