Method to determine the enantiomers of ibuprofen from human urine by high-performance liquid chromatography.
By means of ethyl chloroformate, ibuprofen enantiomers were coupled to 4-methoxyaniline. The resulting amides were resolved from each other and from urinary constituents on a Pirkle column using an isocratic mobile phase with ultraviolet detection at 254 nm. Applicability of this method for the determination of inter-individual differences in urinary metabolic profiles of ibuprofen enantiomers is demonstrated. The chromatographic behavior of the corresponding amide derivatives of two ibuprofen metabolites is also described.
Nicoll-Griffith
D A
DA
Department of Pharmacology, University of Toronto, Ontario, Canada.
Inaba
T
T
Tang
B K
BK
Kalow
W
W
D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D006868:Hydrolysis; D007052:Ibuprofen / Q000652:urine*; D013237:Stereoisomerism
D007052:Ibuprofen