Alveolar and poorly differentiated rhabdomyosarcoma. A clinicopathologic, light-microscopic, ultrastructural and immunohistochemical analysis.
Eighteen poorly differentiated, small and dark cell malignancies afflicting young individuals without light-microscopic evidence of a rhabdomyoblastic differentiation or a growth pattern characteristic of rhabdomyosarcoma were analyzed and compared with a series of 30 alveolar rhabdomyosarcomas of varying differentiation, where the diagnosis could be established light-microscopically. The study comprised clinical data, light and electron microscopy and immunohistochemistry, using a battery of mono- and polyclonal antibodies against intermediate filaments, myoglobin, epithelial membrane antigen, neuron-specific enolase, S-100 and leucocyte common antigen. All 30 alveolar rhabdomyosarcomas were positive for desmin, while a minority were positive for myoglobin, using monoclonal antibodies. In 8 of the 18 small and dark cell malignancies, support for a rhabdomyoblastic differentiation was obtained by a positive staining for desmin. In only 3 of these 8 cases was there ultrastructural evidence of rhabdomyosarcoma. The results of the investigation indicate that immunohistochemistry is a more useful tool than electron microscopy in the diagnosis of poorly differentiated rhabdomyosarcoma and that the criteria for the diagnosis of poorly differentiated rhabdomyosarcoma may need to be reformulated.
Seidal
T
T
Department of Pathology, Gothenburg University, Sahlgrenska Sjukhuset, Sweden.
Kindblom
L G
LG
Angervall
L
L
D000911:Antibodies, Monoclonal; D002454:Cell Differentiation; D003893:Desmin / Q000276:immunology; D006801:Humans; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D009211:Myoglobin / Q000276:immunology; D010751:Phosphopyruvate Hydratase / Q000276:immunology; D012208:Rhabdomyosarcoma / Q000175:diagnosis / Q000276:immunology / Q000473:pathology* / Q000648:ultrastructure; D014746:Vimentin / Q000276:immunology
D000911:Antibodies, Monoclonal; D003893:Desmin; D009211:Myoglobin; D014746:Vimentin; D010751:Phosphopyruvate Hydratase