Assay of alpha-D-galactosyltransferase of subgroup B sera.
Carriers of weak B antigen were found in three generations of a family. The red cells of the propositus reacted with anti-A human serum and Dolichos biflorus lectin as strongly as normal A1B red cells, but they agglutinated at 8-fold dilution against anti-B human serum (1:128) and did not have a mixed-field agglutination. The red cells of her niece agglutinated at 32-fold dilution against the same anti-B serum and did not have a mixed-field pattern. Her red cells were provisionally designated B2, analogous to A2 of subgroup A. B antigen of the propositus appeared clearly depressed, and she was provisionally designated A1B2. When papain-treated O red cells were used as acceptors instead of untreated O red cells, group A1B2 sera could convert them into B-active cells, which were agglutinated by anti-B human serum. alpha-D-galactosyltransferase activity in A1B2 serum was about one-eighth that in normal B serum.
Kogure
T
T
Department of Legal Medicine, Gunma University School of Medicine, Maebashi, Japan.
Nakajima
T
T
Takeya
A
A
Hosomi
O
O
Furukawa
K
K
D000017:ABO Blood-Group System / Q000235:genetics*; D000328:Adult; D005260:Female; D005700:Galactosyltransferases / Q000097:blood* / Q000172:deficiency; D006386:Hemagglutination Tests; D006801:Humans; D007106:Immune Sera; D037102:Lectins; D008297:Male; D010375:Pedigree; D037121:Plant Lectins
D000017:ABO Blood-Group System; D007106:Immune Sera; D037102:Lectins; D037121:Plant Lectins; C036554:dolichos biflorus agglutinin; D005700:Galactosyltransferases; C023155:blood-group-substance alpha-D-galactosyltransferase