[The effect of calcium and magnesium ions on the interaction of calcium-binding proteins parvalbumin and alpha-lactalbumin with dipalmitoylphosphatidylcholine vesicles].
Interactions of the calcium binding proteins, like parvalbumins pI 4.2 and p15.0 and bovine and human alpha-lactalbumins, with dipalmitoylphosphatidylcholine vesicles have been studied by means of scanning microcalorimetry and intrinsic tryptophan, tyrosine and phenylalanine fluorescence. The interactions are modulated by the Ca2+ and Mg2+ binding to the proteins and induce some changes in the physical properties of both the proteins and the liposomes. The liposomes increase the thermal stability of the Mg2+-loaded and metal-free parvalbumin. Ca2+-loaded alpha-lactalbumin interacts with the liposomes in its native state, while the metal-free protein binds to the liposomes mainly in its thermally denatured state. The interactions of both proteins with the liposomes affect the phase transition from gel to liquid-crystalline state in the liposomes. The results of the microcalorimetric and spectrofluorometric studies are corroborated by the data obtained by means of gel-chromatography on Sepharose 4B.
Permiakov
E A
EA
Kreĭmer
D I
DI
Kalinichenko
L P
LP
Shnyrov
V L
VL
D015060:1,2-Dipalmitoylphosphatidylcholine; D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D002417:Cattle; D002850:Chromatography, Gel; D006801:Humans; D007768:Lactalbumin / Q000378:metabolism*; D008081:Liposomes; D008274:Magnesium / Q000494:pharmacology*; D009124:Muscle Proteins / Q000378:metabolism*; D010320:Parvalbumins / Q000378:metabolism*; D011489:Protein Denaturation; D012487:Salmonidae; D013050:Spectrometry, Fluorescence
D002135:Calcium-Binding Proteins; D008081:Liposomes; D009124:Muscle Proteins; D010320:Parvalbumins; D015060:1,2-Dipalmitoylphosphatidylcholine; D007768:Lactalbumin; D008274:Magnesium; D002118:Calcium
Vliianie ionov kal'tsiia i magniia na vzaimodeĭstvie kal'tsiĭsviazyvaiushchikh belkov parval'bumina i alpha-laktal'bumina s vezikulami dipal'mitoilfosfatidilkholina.