title: Cyclophosphamide-induced suppressor cells in mice: suppression of the antibody response in vitro and characterization of the effector cells.
abstract: It was found earlier that nonspecific suppressor cells obtained from the spleen of mice injected with cyclophosphamide (Cy) at a dose of 200 mg/kg body wt are nonadherent, surface Ig negative, and Thy-1 negative. It is now reported that Cy-induced suppressor (Cy-S) cells suppress the in vitro primary and secondary humoral responses in a dose-dependent manner. Suppressor activity is a property of a low density cell that is found in the spleen from 5 to 11 days after Cy administration. Cy-S cells are negative for a number of T-cell markers and are nonadherent to plastic. They are not agglutinated by peanut agglutinin and do not acquire the Thy-1 antigen after treatment with thymosin, suggesting that they are not T-cell precursors. While NK activity was detected in the Cy-S cell preparations, this activity was removed by treatment with antibodies specific for the Qa-5 antigen and complement without affecting the suppressor activity. Cy-S cells are, therefore, distinct from B cells, T cells, NK cells, and macrophages.
authors.forename: M
authors.initials: M
authors.lastname: Segre
authors.forename: E
authors.initials: E
authors.lastname: Tomei
authors.forename: D
authors.initials: D
authors.lastname: Segre
mesh_terms: D000818:Animals; D000917:Antibody Formation / Q000187:drug effects*; D002448:Cell Adhesion; D003520:Cyclophosphamide / Q000494:pharmacology*; D005260:Female; D006183:H-2 Antigens; D066298:In Vitro Techniques; D007700:Kinetics; D051379:Mice; D008815:Mice, Inbred Strains; D013154:Spleen / Q000276:immunology; D013234:Stem Cells / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000187:drug effects* / Q000276:immunology
chemical_list: D006183:H-2 Antigens; D003520:Cyclophosphamide