Biological and biochemical activities of a toxoid of erythrogenic toxin type A.
A toxoid of erythrogenic toxin type A (ET A) was prepared by formaldehyde treatment. Already 15 min after exposure to formaldehyde in isoelectric focusing the ET A band at pH 5.2 shifted to a band at pH 4.5. In Ouchterlony double diffusion test ET A and its toxoid were found to be identical, in fused rocket immuno-electrophoresis a reaction of partial identity was seen. Formaldehyde treatment of ET A resulted in an apparent increase of electrophoretic mobility. In contrast to ET A, its toxoid is non-mitogenic, non-pyrogenic and has lost its ability to induce delayed type hypersensitivity. Binding of ET A toxoid to human peripheral lymphocytes is of the same magnitude as binding of gold-labelled ET A.
Knöll
H
H
Academy of Sciences of the GDR, Central Institute of Microbiology and Experimental Therapy, DDR, Jena, German Democratic Republic.
Gerlach
D
D
Köhler
W
W
Holm
S E
SE
Wagner
B
B
D000818:Animals; D001426:Bacterial Proteins; D004591:Electrophoresis, Polyacrylamide Gel; D005098:Exotoxins / Q000276:immunology / Q000378:metabolism / Q000633:toxicity; D006801:Humans; D006968:Hypersensitivity, Delayed; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D007525:Isoelectric Focusing; D007700:Kinetics; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000378:metabolism; D008565:Membrane Proteins; D011749:Pyrogens / Q000276:immunology / Q000378:metabolism / Q000633:toxicity; D011817:Rabbits; D013297:Streptococcus pyogenes; D014121:Toxoids / Q000032:analysis / Q000276:immunology / Q000633:toxicity
D001426:Bacterial Proteins; D005098:Exotoxins; D008565:Membrane Proteins; D011749:Pyrogens; C117980:SpeA protein, Streptococcus pyogenes; D014121:Toxoids; C027951:erythrogenic toxin