Follow-up studies on proliferative T cell responses in cadaveric kidney graft recipients under combined immunosuppressive treatment with cyclosporine and prednisone.
In 5 recipients of cadaveric renal allografts, we tested the influence of prophylactic immunosuppression with cyclosporine (Cy A) and low dose prednisone on in vitro proliferative T cell responses, T-helper/T-suppressor cell ratios and spontaneous- or lectin-induced unspecific suppressor cell activity and on serum mediated inhibition of proliferative T cell responses. Results revealed a reduction of the overall proliferative T cell responsiveness, which was particularly seen in cultures supplemented with autologous serum and was maximally expressed after approximately 30 days of treatment. This impaired proliferative capacity was neither accompanied by shifts of the T-helper/T-suppressor ratios nor by alterations of spontaneous- or lectin-induced suppressor activity. The capacity of patients' plasma to inhibit lymphocyte proliferation was also tested. Results indicated that almost every plasma of Cy A patients was capable of inhibiting mixed lymphocyte culture (MLC) responses. The inhibitory capacities of these plasma, however, were not directly correlated with their Cy A content.
Irschick
E U
EU
Niederwieser
D
D
Lochs
A
A
Spielberger
M
M
Gattringer
C
C
Margreiter
R
R
Huber
C
C
D000328:Adult; D003524:Cyclosporins / Q000627:therapeutic use*; D006801:Humans; D007165:Immunosuppression Therapy; D066298:In Vitro Techniques; D016030:Kidney Transplantation; D008213:Lymphocyte Activation / Q000187:drug effects*; D008297:Male; D008875:Middle Aged; D011241:Prednisone / Q000627:therapeutic use*; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology
D003524:Cyclosporins; D011241:Prednisone