A simple in vivo bioassay for inhibin-like activity using ovariectomized ewes.
Long term ovariectomized ewes were used in a bioassay for inhibin-like activity. The concentration of FSH 6 to 7 hr after injection of follicular fluid (a rich source of inhibin), as a percentage of pretreatment, regressed on the log of the dose had a slope of -26.0 +/- 7.6 (5 replications, mean +/- SD) and an index of precision of .32 +/- .04. This system was rapid, relatively easy and specific for in vivo inhibin-like activity. This bioassay was also used to determine the relative potency of an affinity-purified fraction of follicular fluid.
Miller
K F
KF
U.S. Department of Agriculture, Agricultural Research Service, Beltsville, MD 20705.
Bolt
D J
DJ
D000818:Animals; D001681:Biological Assay / Q000379:methods; D001826:Body Fluids / Q000502:physiology; D002417:Cattle; D005260:Female; D005640:Follicle Stimulating Hormone / Q000032:analysis*; D007265:Inhibins / Q000032:analysis*; D006080:Ovarian Follicle / Q000502:physiology; D010052:Ovariectomy / Q000662:veterinary; D012756:Sheep / Q000502:physiology*
D007265:Inhibins; D005640:Follicle Stimulating Hormone