title: Synergistic effect of dosage and bacterial inoculum in TEM-1 mediated antibiotic resistance.
abstract: The effect of inoculum size and gene dosage on the level of antibiotic resistance mediated by TEM-1 beta-lactamase was measured. From the results it seemed that gene dosage is a more efficient mechanism than inoculum size for increasing TEM-1 mediated resistance to beta-lactam antibiotics. It also seemed that the two mechanisms for enhancing antibiotic resistance are synergistic. The clinical implications of these results are discussed.
authors.affiliation: Servicio de Microbiologia, Hospital Ramon y Cajal, Madrid, Spain.
authors.forename: J A
authors.initials: JA
authors.lastname: Reguera
authors.forename: F
authors.initials: F
authors.lastname: Baquero
authors.forename: J C
authors.initials: JC
authors.lastname: Perez-Diaz
authors.forename: J L
authors.initials: JL
authors.lastname: Martinez
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D004352:Drug Resistance, Microbial / Q000235:genetics; D005798:Genes, Bacterial; D008826:Microbial Sensitivity Tests; D010957:Plasmids; D001618:beta-Lactamases / Q000096:biosynthesis* / Q000235:genetics; D047090:beta-Lactams
chemical_list: D000900:Anti-Bacterial Agents; D047090:beta-Lactams; D001618:beta-Lactamases