Difference in the production of human interferon-alpha and -beta in mouse cells.
Human interferon-alpha 1 and interferon-beta genes with their flanking regions were introduced into mouse LMTK- cells. Although transfected cells contained the interferon genes with a similar copy number and produced a similar amount of interferon-specific mRNA, cells containing the human interferon-beta gene secreted about 10 times more human interferon than cells transfected with the human interferon-alpha 1 gene. When the coding region of the interferon-beta gene was replaced by that of the interferon-alpha 1 gene (hybrid interferon beta/alpha gene), the human interferon production of transfected cells fell by approx. one order of magnitude. These results show that in the case of exogenous interferon genes a translational or post-translational mechanism might significantly affect the final level of human interferons, resulting in higher titres of interferon-beta than of interferon-alpha.
Toth
M I
MI
Institute of Biochemistry, Hungarian Academy of Sciences, Szeged.
Cendsuren
O
O
Endresz
V
V
Karcagi
I
I
Duda
E
E
D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D002999:Clone Cells; D005786:Gene Expression Regulation; D005796:Genes; D006801:Humans; D007370:Interferon Type I / Q000096:biosynthesis* / Q000235:genetics; D051379:Mice; D010957:Plasmids; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis*; D015183:Restriction Mapping; D014170:Transformation, Genetic
D007370:Interferon Type I; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins