Salt soluble cross-linked elastin: formation and composition of fibers.
Cross-linked elastin has been isolated from the salt extract of sheep vascular tissue by means of hydrophobic interaction chromatography on a column of decyl-agarose. Dialysis of the dimethylformamide and sodium dodecyl sulphate column eluates against distilled water produced a precipitate that was fibrous and that resembled insoluble elastin fibers. As judged by amino acid analyses and SDS-polyacrylamide gel electrophoresis, this precipitation resulted in further purification of the soluble cross-linked elastin. Similar chromatography and precipitation of oxalic acid solubilized cross-linked elastin (alpha-elastin) produced identical results.
Manning
J N
JN
Davis
P F
PF
Greenhill
N S
NS
Sigley
A J
AJ
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001808:Blood Vessels / Q000032:analysis; D002847:Chromatography, Agarose; D003956:Dialysis; D004549:Elastin / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D008855:Microscopy, Electron, Scanning; D011487:Protein Conformation; D012756:Sheep; D012965:Sodium Chloride; D012995:Solubility
D000596:Amino Acids; D012965:Sodium Chloride; D004549:Elastin