Comparative analysis of oncogene expression and chromosome abnormalities between metastatic and nonmetastatic B16 melanoma clones.
We have established nonmetastatic mouse B16 melanoma clone, C1-2, and metastatic variant, C4-1, by subcloning the 30th passage of nonmetastatic W1-4 cells. We have investigated oncogene expression and chromosomal abnormalities to see whether there is some correlation with tumor progression and phenotypic diversification in melanoma cells. Not only metastatic C4-1 but also nonmetastatic C1-2 showed similarly high expression of c-Ha-ras and c-myc oncogenes by Northern blotting method. The chromosome numbers of both clones were distributed mainly within the range of 48-52. C1-2 had about 20 biarmed chromosomes and C4-1 had 13 or 14, which made real ploidy of C1-2 and C4-1, hypotetraploidy and triploidy, respectively. 21 marker chromosomes were commonly observed, while 9 marker chromosomes peculiar to C1-2 and 4 peculiar to C4-1 were constantly recognized. In partial accordance with a previous report, chromosomes 4, 5, 6, 8, 10, 13, 18, 19, and X were additionally observed in nonmetastatic C1-2 cells, compared with metastatic C4-1 cells. Furthermore, in our study, the increase of chromosomes 1, 15, and 16 was found to be greater in metastatic C4-1.
Funasaka
Y
Y
Department of Dermatology, Kobe University School of Medicine, Japan.
Mishima
Y
Y
Ichihashi
M
M
Sugiyama
T
T
D000818:Animals; D002460:Cell Line; D002869:Chromosome Aberrations; D002999:Clone Cells; D005260:Female; D007621:Karyotyping; D008175:Lung Neoplasms / Q000235:genetics / Q000556:secondary; D008546:Melanoma, Experimental / Q000201:enzymology / Q000235:genetics / Q000473:pathology / Q000556:secondary*; D051379:Mice; D008810:Mice, Inbred C57BL; D008819:Mice, Nude; D014442:Monophenol Monooxygenase / Q000378:metabolism; D009857:Oncogenes; D010641:Phenotype
D014442:Monophenol Monooxygenase