Antigenic heterogeneity of metastasizing and nonmetastasizing forms of hamster lymphosarcoma: comparison of primary tumor and spontaneous liver metastases.
Antisera were raised in rabbits against nonmetastasizing (NML) and metastasizing (ML) forms of hamster lymphosarcoma and were purified against normal hamster tissues. Immunoglobulins from the purified antisera were precipitated with 1.6 M ammonium sulfate, radioiodinated and IgG separated by Sephadex G-200 gel filtration. 125I-IgG preparations were analyzed by a direct cell binding assay and by a complement-dependent cytotoxicity test, employing single cell suspensions from primary lymphosarcoma. In some experiments, 125I-IgG was also tested against ML cells obtained from primary tumor (1 degree) and its liver metastasis (2 degrees). NML induced greater anti-tumor antibody production than ML, suggesting greater antigenicity of the non-metastasizing tumor. The two lymphosarcomas appeared to share some common tumor-associated antigens since antibody to one tumor type was either completely or partially absorbed by tumor cells of the other type. Anti-ML 125I-IgG proved up to 2-5 times more cytotoxic for ML 1 degree than 2 degrees cells. Although both tumors were highly tumorigenic in hamsters, only ML gave rise to distant metastases, predominantly in the liver.
Shah
S A
SA
Sherbet
G V
GV
Lakshmi
M S
MS
Wildridge
M
M
D000818:Animals; D000951:Antigens, Neoplasm / Q000032:analysis*; D006224:Cricetinae; D003602:Cytotoxicity, Immunologic; D005779:Immunodiffusion; D008113:Liver Neoplasms / Q000276:immunology / Q000556:secondary*; D008228:Lymphoma, Non-Hodgkin / Q000276:immunology / Q000556:secondary*; D008297:Male; D008647:Mesocricetus; D017452:Receptors, IgG; D011971:Receptors, Immunologic / Q000378:metabolism
D000951:Antigens, Neoplasm; D017452:Receptors, IgG; D011971:Receptors, Immunologic