Interrelationship between organelles in kidney cells of adult and developing rat.
The morphologic interrelationships between mitochondria, the endoplasmic reticulum (ER) and other organelles were examined in rat kidney cells by stereomicroscopy of thick sections (0.3-1.0 micron) using either standard transmission (80-100 kV) or high voltage (1 mEV) electron microscopy. Mitochondria fit into three different categories: (1) elongated cylinders observed in S1 and S2 segments; (2) irregular lamina in the cortical ascending limb or plates in the distal straight and convoluted segments; (3) small spheres or short rods mostly in intercalated and principal cells of the collecting tubule. The chondrioma occupies a large volume in all cells except in the thin limb and in principal cells of the collecting tubule. This volume occupied by the chondrioma is likely to be related to metabolic functions, but its polymorphic configuration could also be explained by a passive adaptation of the mitochondria to the space left by the basilar membrane infoldings and the ER network, which was found to have an extensive three-dimensional organization which varies, as for the mitochondria, with the cell type. In fact, in the proximal nephron, mitochondria surrounded by the ER and the plasma membranes appear to form a functional unit. Organelle interrelationship is extensively modified during ontogeny or under hormonal influence. Disruption in organelle relationships or in their motility could probably have far reaching consequences which would be more deleterious than the pathological lesions observed at the level of a single organelle. The extent of the disassembly can be fully explored only with thick sections and stereomicroscopy.
Bergeron
M
M
Department of Physiology, Faculty of Medicine, University of Montreal, Quebec, Canada.
Gaffiero
P
P
Berthelet
F
F
Thiery
G
G
D000818:Animals; D002462:Cell Membrane / Q000648:ultrastructure; D004721:Endoplasmic Reticulum / Q000648:ultrastructure; D005333:Fetus / Q000648:ultrastructure; D007668:Kidney / Q000196:embryology / Q000254:growth & development / Q000648:ultrastructure*; D008854:Microscopy, Electron; D008928:Mitochondria / Q000648:ultrastructure; D015388:Organelles / Q000648:ultrastructure*; D051381:Rats; D011919:Rats, Inbred Strains