Functional capacities of chick embryo thymocytes in the mixed lymphocyte reaction.
Thymocytes from chick embryos homozygous for the B19 haplotype of the major histocompatibility locus were tested in a one way MLR either as responder or stimulator cells, against adult peripheral blood lymphocytes from B14/14 or B19/19 strains. 13-day embryonic thymocytes were strongly stimulated by adult allogeneic PBL. By contrast 16-day thymocytes were unresponsive. This difference might be related to the rhythmic waves of stem cell entry and multiplication which characterize the ontogeny of the avian thymus. Mitomycin-treated thymocytes from 13-day or 16-day embryos were both efficient in stimulating thymidine uptake by adult allogeneic PBL.
Cormier
F
F
Dieterlen-Lievre
F
F
D000818:Animals; D002642:Chick Embryo / Q000276:immunology*; D002645:Chickens / Q000276:immunology; D006649:Histocompatibility Antigens; D066298:In Vitro Techniques; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D008934:Mitogens / Q000494:pharmacology; D008937:Mitomycins / Q000494:pharmacology; D013234:Stem Cells / Q000276:immunology; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology*
D006649:Histocompatibility Antigens; D008934:Mitogens; D008937:Mitomycins