Enhancement by recombinant interferon-gamma of spontaneous tumor cytostasis by human neutrophils.
Human peripheral neutrophils treated with recombinant human interferon-gamma (IFN) inhibited tritiated thymidine (3H-TdR) uptake by various tumor cells. The concentration-response curve of IFN required for induction of cytostatic activity of neutrophils showed two peaks. Short time incubation of neutrophils with IFN at 4 degrees C was sufficient for inducing neutrophil cytostasis. When neutrophils pretreated with IFN for 5 min at 4 degrees C were further treated with trypsin, cytostasis by the treated neutrophils was decreased depending on the concentration of trypsin, whereas cytostasis by neutrophils pretreated with IFN for 180 min at 37 degrees C was not inhibited by trypsin treatment. Cytostatic activity induced by IFN was inhibited by pretreatment of IFN with anti-IFN-monoclonal antibody.
Miyake
Y
Y
Department of Parasitology, Yamagata University School of Medicine, Japan.
Ajitsu
S
S
Yamashita
T
T
Sendo
F
F
D000818:Animals; D000911:Antibodies, Monoclonal; D003602:Cytotoxicity, Immunologic / Q000187:drug effects*; D004306:Dose-Response Relationship, Immunologic; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D007700:Kinetics; D004915:Leukemia, Erythroblastic, Acute / Q000276:immunology; D008223:Lymphoma / Q000276:immunology; D051379:Mice; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology*; D011112:Polymyxin B / Q000494:pharmacology; D011994:Recombinant Proteins; D013696:Temperature; D014357:Trypsin; D014407:Tumor Cells, Cultured / Q000276:immunology*
D000911:Antibodies, Monoclonal; D011994:Recombinant Proteins; D007371:Interferon-gamma; D014357:Trypsin; D011112:Polymyxin B