title: Method to determine the enantiomers of ibuprofen from human urine by high-performance liquid chromatography.
abstract: By means of ethyl chloroformate, ibuprofen enantiomers were coupled to 4-methoxyaniline. The resulting amides were resolved from each other and from urinary constituents on a Pirkle column using an isocratic mobile phase with ultraviolet detection at 254 nm. Applicability of this method for the determination of inter-individual differences in urinary metabolic profiles of ibuprofen enantiomers is demonstrated. The chromatographic behavior of the corresponding amide derivatives of two ibuprofen metabolites is also described.
authors.affiliation: Department of Pharmacology, University of Toronto, Ontario, Canada.
authors.forename: D A
authors.initials: DA
authors.lastname: Nicoll-Griffith
authors.forename: T
authors.initials: T
authors.lastname: Inaba
authors.forename: B K
authors.initials: BK
authors.lastname: Tang
authors.forename: W
authors.initials: W
authors.lastname: Kalow
mesh_terms: D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D006868:Hydrolysis; D007052:Ibuprofen / Q000652:urine*; D013237:Stereoisomerism
chemical_list: D007052:Ibuprofen