Isolation of human complement factors C3, C5 and H.
An improved method for simultaneous purification of complement factors C3, C5 and H from human plasma has been developed. Using an initial batch separation technique with QAE-Sephadex, followed by chromatography on SP-Sephadex and gel filtration in Sephadex G-200, 600 mg of highly pure C3 can be prepared from 1600 ml of plasma. Simultaneously about 70 mg of highly pure factor H and 30 mg of C5 are obtained by chromatography of post SP-Sephadex material on DEAE-Sephacel. A small amount of C3 in the C5 pool is removed by anti-C3-Sepharose. By maleylation or citraconylation of reduced and alkylated C3, the constitutive polypeptide chains are modified in a way that made them separable by ion exchange chromatography.
Lundwall
A
A
Eggertsen
G
G
D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003176:Complement C3 / Q000302:isolation & purification*; D003180:Complement C3b Inactivator Proteins / Q000302:isolation & purification*; D003182:Complement C5 / Q000302:isolation & purification*; D017242:Complement Factor H; D006801:Humans; D010446:Peptide Fragments / Q000302:isolation & purification
C493495:CFH protein, human; D003176:Complement C3; D003180:Complement C3b Inactivator Proteins; D003182:Complement C5; D010446:Peptide Fragments; D017242:Complement Factor H