Purification and characterization of human plasma Zn-alpha 2-glycoprotein.
Zn-alpha 2-glycoprotein (Zn alpha 2gp) was purified from fresh human plasma approximately 670-fold in a yield of 18% over the fractions from DEAE-Sephadex A-50 column chromatography. The purified protein was a glycoprotein with molecular weights of 56,000 and 57,000 on Superose and Sephadex G-150 column chromatographies and of 41,000 and 42,000 on nonreduced SDS-PAGE. Characterization, which included a determination of molecular weight, amino acid composition, amino terminus, and antigenicity, correlated well with known values previously reported for human Zn alpha 2gp.
Ohkubo
I
I
Department of Biochemistry, Nagoya City University Medical School, Japan.
Niwa
M
M
Sasaki
M
M
D000596:Amino Acids / Q000032:analysis; D002241:Carbohydrates / Q000032:analysis; D002848:Chromatography, DEAE-Cellulose; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000097:blood / Q000302:isolation & purification*; D006801:Humans; D005779:Immunodiffusion; D029589:Seminal Plasma Proteins; D000090203:Zn-Alpha-2-Glycoprotein
D000596:Amino Acids; D002241:Carbohydrates; D006023:Glycoproteins; D029589:Seminal Plasma Proteins; D000090203:Zn-Alpha-2-Glycoprotein