title: Serum antibody response to filamentous hemagglutinin in patients with clinical pertussis measured by an enzyme-linked immunosorbent assay.
abstract: Titers of antibodies to filamentous hemagglutinin (FHA) were determined by enzyme-linked immunosorbent assay in acute and convalescent phase serum samples from 158 patients with clinical symptoms typical of whooping-cough. In 96 of the patients the diagnosis was verified by culture. Significant changes in serum levels of IgG, IgM and/or IgA antibodies against FHA were demonstrated in 126 patients (80%). Thus, demonstration of significant changes in FHA antibody titers in serum can be used for serological diagnosis of pertussis. The results also show that high levels of IgG, IgM and/or IgA antibodies in a single serum sample suggest current pertussis infection, but if the diagnosis is based on determinations of FHA antibody titers in a single serum sample the sensitivity is low. The levels of antibody to FHA were compared with previously determined levels of antibodies to pertussis toxin. A significant antibody response against both FHA and pertussis toxin was seen in 111 patients (70%) while 147 patients (93%) developed a significant increase in antibodies against one or both antigens.
authors.affiliation: Department of Clinical Bacteriology, University of Göteborg, Sweden.
authors.forename: G
authors.initials: G
authors.lastname: Zackrisson
authors.forename: F
authors.initials: F
authors.lastname: Arminjon
authors.forename: I
authors.initials: I
authors.lastname: Krantz
authors.forename: T
authors.initials: T
authors.lastname: Lagergård
authors.forename: N
authors.initials: N
authors.lastname: Sigurs
authors.forename: J
authors.initials: J
authors.lastname: Taranger
authors.forename: B
authors.initials: B
authors.lastname: Trollfors
mesh_terms: D000293:Adolescent; D000328:Adult; D000907:Antibodies, Bacterial / Q000032:analysis*; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D006388:Hemagglutinins / Q000276:immunology*; D006801:Humans; D007136:Immunoglobulins / Q000032:analysis; D007223:Infant; D037342:Pertussis Toxin; D010566:Virulence Factors, Bordetella / Q000276:immunology; D014917:Whooping Cough / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D006388:Hemagglutinins; D007136:Immunoglobulins; D010566:Virulence Factors, Bordetella; D037342:Pertussis Toxin