title: [An optimal method of determining the level of cathepsin A in blood platelet homogenates].
abstract: Blood platelet cathepsin A hydrolyzes Cbz-Glu-Tyr most intensively at pH 5.0, it does so too, with Cbz-Glu-Phe, but to a smaller extent. Using the ninhydrin method for estimation of the released tyrosine++ or phenylalanine previous elimination of amino acids++ and low-molecular peptides is necessary as it is too including alpha-amine nitrogen released by platelet proteases from peptides and proteins.
authors.affiliation: Zakład Analizy Instrumentalnej AM w Białymstoku.
authors.forename: H
authors.initials: H
authors.lastname: Ostrowska
mesh_terms: D000818:Animals; D001792:Blood Platelets / Q000201:enzymology*; D002021:Buffers; D002268:Carboxypeptidases / Q000097:blood*; D043402:Cathepsin A; D002403:Cathepsins / Q000097:blood*; D002478:Cells, Cultured; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D066298:In Vitro Techniques; D008247:Lysosomes / Q000201:enzymology*; D013552:Swine
chemical_list: D002021:Buffers; D002268:Carboxypeptidases; D002403:Cathepsins; D043402:Cathepsin A
vernacular_title: Optymalizacja metody oznaczania katepsyny A w homogenacie płytek krwi.