Persistent stimulation of adenylate cyclase and urea transport by an AVP photolabel.
The effects of a photoaffinity label for arginine vasopressin receptors, [Phe2, Phe(p-N3)3]AVP (N3-AVP), on urea permeability and adenylate cyclase activity have been investigated in the toad urinary bladder. This compound, when activated by ultraviolet light, induced a maximal and persistent increase in the urea permeability of the intact bladder and a persistent increase in the adenylate cyclase activity of toad bladder epithelial cell homogenates. Covalent attachment of the analogue to target tissue during photolysis was equivalent at 4 and 20 degrees C. Bladders exposed to N3-AVP in the presence of AVP during photolysis were substantially less permeable to urea than controls that had been exposed to N3-AVP alone. These findings constitute further evidence in support of our previous suggestion that N3-AVP binds covalently to AVP receptors and, in addition, demonstrates that N3-AVP evokes a persistent increase in adenylate cyclase activity which, in turn, triggers a persistent increase in bladder permeability to urea.
Eggena
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Fahrenholz
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Schwartz
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D000262:Adenylyl Cyclases / Q000378:metabolism*; D000345:Affinity Labels / Q000494:pharmacology*; D000818:Animals; D001127:Arginine Vasopressin / Q000031:analogs & derivatives* / Q000494:pharmacology; D001692:Biological Transport / Q000187:drug effects; D002024:Bufo marinus; D005260:Female; D066298:In Vitro Techniques; D010539:Permeability; D010777:Photochemistry; D011945:Receptors, Angiotensin / Q000378:metabolism; D017483:Receptors, Vasopressin; D013696:Temperature; D014466:Ultraviolet Rays; D014508:Urea / Q000378:metabolism*; D001743:Urinary Bladder / Q000187:drug effects* / Q000378:metabolism
D000345:Affinity Labels; D011945:Receptors, Angiotensin; D017483:Receptors, Vasopressin; D001127:Arginine Vasopressin; C041401:argipressin, Phe(2)-(4-azido)Phe(3)-; D014508:Urea; D000262:Adenylyl Cyclases