title: Phospholipids from the hepatopancreas of Indian horseshoe crab Carcinoscropius rotundicauda.
abstract: Total phospholipids were extracted from the heart, hepatopancreas, and hemolymph of the Indian horseshoe crab Carcinoscorpius rotundicauda by the conventional method. Characteristic group reaction and 2-dimensional thin-layer chromatography on silica gel were used for identification of different phospholipids. The phospholipid profile obtained from hemolymph and 2 major organs are comparable and show phosphatidyl choline (PC) and phosphatidyl ethanolamine to be the major phospholipids. A phospholipid has been consistently detected migrating immediately below the PC in the thin-layer chromatogram of lipids extracted from the hepatopancreas. When mixed methyl esters of this slower moving PC are resolved on a silica gel plate ran in hexane ether:acetic acid 80:20:1, with appropriate controls, an additional spot is seen just below the normal methyl ester, indicating a difference between the fatty acid compositions of 2 PC (e.g., regular and slower). The slower mixed methyl esters were found to comprise mainly the 4 saturated fatty acids: lauric, myristic, palmitic, and stearic. The slow moving PC seems to consist mainly of molecular species with the above-mentioned saturated fatty acids at both Sn 1 and Sn 2 positions.
authors.affiliation: Biomembrane Division, Indian Institute of Chemical Biology, Calcutta.
authors.forename: R
authors.initials: R
authors.lastname: Bandyopadhyay
authors.forename: M K
authors.initials: MK
authors.lastname: Basu
mesh_terms: D000818:Animals; D002855:Chromatography, Thin Layer; D006737:Horseshoe Crabs / Q000378:metabolism*; D008099:Liver / Q000032:analysis; D008196:Lymph / Q000032:analysis; D009206:Myocardium / Q000032:analysis; D010179:Pancreas / Q000032:analysis; D010743:Phospholipids / Q000302:isolation & purification*; D010738:Type C Phospholipases / Q000494:pharmacology
chemical_list: D010743:Phospholipids; D010738:Type C Phospholipases