title: A heritable variant of mouse liver ornithine aminotransferase (EC 2.6.1.13) induced by ethylnitrosourea.
abstract: A variant of ornithine aminotransferase (OAT, EC 2.6.1.13) has been detected in an offspring of a male mouse treated with ethylnitrosourea. The evidence presented to support the identification of the protein variant (ENU 2) as altered OAT includes (a) a corresponding 50% decrease in the abundance of a protein, located one charge unit basic to the variant, which comigrates on two-dimensional gel patterns with purified mouse liver OAT; (b) the binding of anti-rat-OAT antibody to the variant; (c) the increased abundance of the variant protein in the livers of mice fed a high protein diet (85% casein); and (d) purification of the variant through an OAT purification protocol.
authors.affiliation: Biological, Environmental, and Medical Research Division, Argonne National Laboratory, Illinois 60439.
authors.forename: C S
authors.initials: CS
authors.lastname: Giometti
authors.forename: S L
authors.initials: SL
authors.lastname: Tollaksen
authors.forename: M A
authors.initials: MA
authors.lastname: Gemmell
authors.forename: J
authors.initials: J
authors.lastname: Burcham
authors.forename: C
authors.initials: C
authors.lastname: Peraino
mesh_terms: D000818:Animals; D004044:Dietary Proteins / Q000008:administration & dosage / Q000494:pharmacology; D004790:Enzyme Induction; D005038:Ethylnitrosourea / Q000494:pharmacology*; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008297:Male; D051379:Mice; D009154:Mutation; D009953:Ornithine-Oxo-Acid Transaminase / Q000096:biosynthesis* / Q000235:genetics; D000637:Transaminases / Q000096:biosynthesis*
chemical_list: D004044:Dietary Proteins; D000637:Transaminases; D009953:Ornithine-Oxo-Acid Transaminase; D005038:Ethylnitrosourea