title: Immunocytochemical localizations of neuron-specific proteins in the taste bud of the guinea pig.
abstract: The taste buds and their nerves in the guinea pig were immunocytochemically investigated with regard to the localization of spot 35 protein, neuron-specific enolase (NSE), neurofilament protein (NFP), and S-100 protein. The spot 35 protein-like immunoreactivity was confined to slender cells comprising half the number of taste bud cells. NSE-like immunoreactivity was recognized in some taste bud cells as well as nerve fibers both within the taste bud and in the subepithelial connective tissue. The NSE-immunoreactive cells were divided into two cell populations: one weakly and the other intensely immunoreactive. The former cells proved to be spot 35 protein-immunoreactive. Moreover, the cells immunoreactive for both spot 35 protein and NSE were frequently associated with nerve fibers immunostained intensely for NSE. The NFP- and S-100 protein-like immunoreactivities were found in none of cells in the taste bud, but exclusively in the subepithelial neural elements.
authors.affiliation: Department of Oral Anatomy, Nippon Dental University, Niigata, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Yoshie
authors.forename: C
authors.initials: C
authors.lastname: Wakasugi
authors.forename: Y
authors.initials: Y
authors.lastname: Teraki
authors.forename: T
authors.initials: T
authors.lastname: Iwanaga
authors.forename: T
authors.initials: T
authors.lastname: Fujita
mesh_terms: D000818:Animals; D003238:Connective Tissue / Q000033:anatomy & histology; D006168:Guinea Pigs; D007150:Immunohistochemistry; D009417:Nerve Tissue / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D013650:Taste Buds / Q000166:cytology / Q000294:innervation / Q000378:metabolism*
chemical_list: D009419:Nerve Tissue Proteins