Immunoscintigraphy of human ovarian cancer xenografts using a radiolabelled monoclonal antibody to human pregnancy serum-derived immune complexes.
The in vivo imaging of xenografted human ovarian cancer in nude mice with a specific and control radiolabelled monoclonal antibody (MoAb) is described. The specific MoAb was previously raised by immunizing mice with immune complexes derived from late human pregnancy serum. In the first group of mice the specific MoAb 131I-5E3 F(ab')2 was injected, while a second group received equivalent amounts of a control MoAb 131I-UJ13A F(ab')2. The mice were imaged at various times up to a maximum of 2 weeks using a gamma camera, and the tumour to non-tumour (T/NT) ratio was recorded for each group. The T/NT ratio rose to 2.02 in the specific group, while the corresponding ratio in the control group was 0.70. In addition, the count rate in the tumour and non-tumour regions was determined on each imaging occasion. Biological half-lives of the divalent fragments of 5E3 and UJ13A in the tumour were 7.53 days and 0.62 days, respectively. Following sacrifice, the tumours were excised and counted relative to the rest of the animal, and the T/NT ratio was calculated. In vitro results were in direct agreement with those recorded in vivo using the gamma camera. From the results it would appear that the divalent fragment of 5E3, which has been raised to immune complexes derived from late human pregnancy serum, is specific for human ovarian tumour xenografts in the nude mouse model.
Murray
T
T
Radionuclide Dispensary, Western Infirmary, Glasgow, UK.
Elliott
A T
AT
Wheldon
T E
TE
Whateley
T L
TL
Stimson
W H
WH
D000818:Animals; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007457:Iodine Radioisotopes; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D010051:Ovarian Neoplasms / Q000000981:diagnostic imaging*; D011247:Pregnancy / Q000276:immunology*; D011877:Radionuclide Imaging
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D007457:Iodine Radioisotopes