Comparison of hepatic, renal and intestinal bilirubin UDP-glucuronyl transferase activities in rat microsomes.
1. Bilirubin UDP-glucuronyltransferase activity and its dependence on substrate concentrations in rat liver, renal cortex and intestinal mucosa microsomes were studied. 2. Bilirubin monoglucuronide synthesis from unconjugated bilirubin was a higher capacity, lower affinity step in comparison with bilirubin diglucuronide formation in the three tissues tested. 3. Bilirubin glucuronide formation in liver microsomes showed a higher capacity but a lower affinity than extrahepatic ones. Renal cortex and intestinal mucosa exhibited similar kinetics parameters. 4. In vitro bilirubin glucuronidation in renal cortex and intestinal mucosa was quantitatively important as compared with the hepatic one.
Mottino
A D
AD
Instituto de Fisiología Experimental, Universidad Nacional de Rosario, Argentina.
Pellegrino
J M
JM
Guibert
E E
EE
Roma
M G
MG
Rodríguez Garay
E A
EA
D000818:Animals; D001663:Bilirubin / Q000031:analogs & derivatives / Q000096:biosynthesis / Q000378:metabolism; D014453:Glucuronosyltransferase / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000201:enzymology*; D007672:Kidney Cortex / Q000201:enzymology*; D007700:Kinetics; D008297:Male; D008861:Microsomes / Q000201:enzymology; D008862:Microsomes, Liver / Q000201:enzymology*; D051381:Rats; D011919:Rats, Inbred Strains
C016266:bilirubin glucuronate; D014453:Glucuronosyltransferase; D001663:Bilirubin