Mechanism of crosslinking of proteins by glutaraldehyde III. Reaction with collagen in tissues.
Bovine pericardium, a dense collagenous connective tissue, was crosslinked with glutaraldehyde using different modalities of fixation. The degree of crosslinking was evaluated as a function of the ability of CNBr and pronase to solubilize collagen. Our results suggest that glutaraldehyde fixes primarily the surface of the fibers and creates a polymeric network which hinders the further crosslinking of the interstitium of the fiber. When a low concentration of glutaraldehyde was used, a slow time-dependent crosslinking process was observed. This slow process is maintained over a long period of time, greatly beyond that required for the actual penetration of glutaraldehyde to occur.
Cheung
D T
DT
Perelman
N
N
Ko
E C
EC
Nimni
M E
ME
D000447:Aldehydes / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D003094:Collagen / Q000378:metabolism*; D003488:Cyanogen Bromide / Q000494:pharmacology; D005976:Glutaral / Q000378:metabolism*; D011402:Pronase / Q000378:metabolism; D012995:Solubility
D000447:Aldehydes; D003094:Collagen; D011402:Pronase; D003488:Cyanogen Bromide; D005976:Glutaral