Histochemical and electronmicroscopical analysis of muscle fiber in myotomes of teleost fish (Noemacheilus barbatulus L.).
A quantitative histochemical study was carried out on axial musculature of Noemacheilus barbatulus L. On the basis of succinate dehydrogenase (SDH) and myofibrillar ATP-ase activity, 5 types of muscle fibers are described. When the SDH method was used, red, tonic, intermediate, and white muscle fibers were easily observed. However, histochemical reaction for myofibrillar ATP-ase activity, after alkaline preincubation (pH = 10.4), revealed another type of fiber zone laying between the intermediate and white muscle fiber regions and forming a transitional zone. Electron microscopic observation showed significant differences in sarcomere organization and thickness of myosin filaments of the various muscle fiber types.
Kilarski
W
W
Kozlowska
M
M
D000251:Adenosine Triphosphatases / Q000032:analysis; D000818:Animals; D003599:Cytoskeleton / Q000648:ultrastructure; D005260:Female; D005399:Fishes / Q000033:anatomy & histology*; D006651:Histocytochemistry; D008297:Male; D008854:Microscopy, Electron; D009132:Muscles / Q000166:cytology / Q000648:ultrastructure*; D009210:Myofibrils / Q000201:enzymology; D009218:Myosins; D013045:Species Specificity; D013385:Succinate Dehydrogenase / Q000032:analysis
D013385:Succinate Dehydrogenase; D000251:Adenosine Triphosphatases; D009218:Myosins