A cell attachment assay for use in the standardization of serum products.
A culture tube assay has been developed which can be used to measure relative amounts of cell attachment activity present in different batches of serum or serum products. The assay utilizes a transformed line of BHK-21 cells which is highly dependent upon serum factors for attachment when the cells are subjected to mild liquid shear forces (tube rotation of 1 rpm). Approximately fivefold differences in attachment activity were observed between different batches of bovine calf serum, while up to 20-fold differences were observed between different batches of horse serum. Less than twofold differences were seen between different lots of fetal bovine serum. The assay appears to have application as a quality control measure for screening serum products.
Giard
D J
DJ
Cell Culture Center, Massachusetts Institute of Technology, Cambridge 02139.
D000818:Animals; D001769:Blood; D002448:Cell Adhesion; D006224:Cricetinae; D012015:Reference Standards; D014407:Tumor Cells, Cultured