A rapid fluorimetric test for lysozyme with purified fluorescamine-labelled peptidoglycan.
A sensitive and rapid fluorometric lysozyme assay is described. It is based on the hydrolysis of fluorescamine-labelled peptidoglycan from Micrococcus luteus cell walls. Lysozyme levels as low as 0.1 microgram can be detected.
Moser
I
I
Institut für Allgemeine Biochemie, Ludwig Boltzmann Forschungsstelle für Biochemie, Vienna, Austria.
Pittner
F
F
Dworsky
P
P
D001412:Bacillus subtilis; D005450:Fluorescamine; D007202:Indicators and Reagents; D008837:Micrococcus; D009113:Muramidase / Q000032:analysis*; D010457:Peptidoglycan / Q000302:isolation & purification; D013050:Spectrometry, Fluorescence / Q000379:methods
D007202:Indicators and Reagents; D010457:Peptidoglycan; D005450:Fluorescamine; D009113:Muramidase