title: Effect of different anesthetics on immunoreactive atrial natriuretic factor concentrations in rat plasma.
abstract: The effect of different conditions of blood withdrawal and use of different anesthetics on immunoreactive atrial natriuretic factor (IR-ANF) concentrations in plasma was studied in rats. The concentration of IR-ANF in plasma from jugular vein of non-anesthetized conscious rats, cannulated either 24 hr before blood withdrawal was 93.9 +/- 17.1 pg/ml (n = 30); and 48 hr: 81.9 +/- 11.5 pg/ml (n = 29). Immobilization stress (4 hr) increased IR-ANF concentration: 248.0 +/- 80.2 pg/ml (n = 5). Anesthesia by morphine, diethyl-ether, chloral hydrate and ketamine chlorhydrate increased IR-ANF concentrations to 2,443.0 +/- 281.2 pg/ml (n = 24), 806.1 +/- 74.6 pg/ml (n = 64), 224.0 +/- 81.4 pg/ml (n = 20), and 195.0 +/- 20.3 pg/ml (n = 51), respectively. IR-ANF in plasma of sodium-pentobarbital and urethane anesthetized rats was 59.2 +/- 6.7 pg/ml (n = 10) and 42.6 +/- 8.1 pg/ml (n = 8), respectively. These changes in IR-ANF evoked by different types of anesthetics and different conditions of blood withdrawal have to be taken into consideration during studies on the physiopathological role of atrial natriuretic factor.
authors.forename: K
authors.initials: K
authors.lastname: Horký
authors.forename: J
authors.initials: J
authors.lastname: Gutkowska
authors.forename: R
authors.initials: R
authors.lastname: Garcia
authors.forename: G
authors.initials: G
authors.lastname: Thibault
authors.forename: J
authors.initials: J
authors.lastname: Genest
authors.forename: M
authors.initials: M
authors.lastname: Cantin
mesh_terms: D000777:Anesthetics / Q000494:pharmacology*; D000818:Animals; D009320:Atrial Natriuretic Factor; D001800:Blood Specimen Collection / Q000379:methods*; D002697:Chloral Hydrate / Q000494:pharmacology; D004986:Ether / Q000494:pharmacology; D005260:Female; D007649:Ketamine / Q000494:pharmacology; D009020:Morphine / Q000494:pharmacology; D009124:Muscle Proteins / Q000097:blood*; D010424:Pentobarbital / Q000494:pharmacology; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D000777:Anesthetics; D009124:Muscle Proteins; C472610:atrial natriuretic peptide, rat; D004986:Ether; D002697:Chloral Hydrate; D007649:Ketamine; D009020:Morphine; D009320:Atrial Natriuretic Factor; D010424:Pentobarbital