Novel Jun- and Fos-related proteins in Drosophila are functionally homologous to enhancer factor AP-1.
A homolog of mammalian enhancer binding factor AP-1 was detected in Drosophila and was purified from embryo nuclear extracts by sequence-specific DNA affinity chromatography. The purified fraction, dAP-1, displays the sequence specificity as well as transcriptional activation properties of mammalian AP-1 and consists of two major proteins of mol. wts 40 and 70 kd. Antibody cross-reactivity experiments suggest that these proteins are Drosophila homologs of proto-oncogene products, Jun and Fos. The Drosophila Jun- and Fos-related antigens, when separated, are individually capable of sequence-specific DNA binding, and the Jun-related antigen activates transcription in vitro.
Perkins
K K
KK
Howard Hughes Medical Institute, Department of Biochemistry, University of California, Berkeley, CA 94720.
Dailey
G M
GM
Tjian
R
R
D000818:Animals; D000941:Antigens / Q000235:genetics / Q000302:isolation & purification; D001665:Binding Sites; D005075:Biological Evolution; D004247:DNA / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D004330:Drosophila / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D006801:Humans; D008970:Molecular Weight; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000302:isolation & purification; D016755:Proto-Oncogene Proteins c-jun; D013045:Species Specificity; D014157:Transcription Factors / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D014158:Transcription, Genetic
D000941:Antigens; D004268:DNA-Binding Proteins; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins; D016755:Proto-Oncogene Proteins c-jun; D014157:Transcription Factors; D004247:DNA