Structure of the precursor to an enzyme mediating COOH-terminal amidation in peptide biosynthesis.
Many bioactive peptides terminate with an amino acid alpha-amide at their COOH terminus. The enzyme responsible for this essential posttranslational modification is known as peptidyl-glycine alpha-amidating monooxygenase or PAM. We identified cDNAs encoding the enzyme by using antibodies to screen a bovine intermediate pituitary lambda gt11 expression library. Antibodies to a beta-galactosidase/PAM fusion protein removed PAM activity from bovine pituitary homogenates. The 108,207 dalton protein predicted by the complete cDNA is approximately twice the size of purified PAM. An NH2-terminal signal sequence and short propeptide precede the NH2 terminus of purified PAM. The sequences of several PAM cyanogen bromide peptides were localized in the NH2-terminal half of the predicted protein. The cDNA encodes an additional 430 amino acid intragranular domain followed by a putative membrane spanning domain and a hydrophilic cytoplasmic domain. The forms of PAM purified from bovine neurointermediate pituitary may be generated by endoproteolytic cleavage at a subset of the 10 pairs of basic amino acids in the precursor. High levels of PAM mRNA were found in bovine pituitary and cerebral cortex. In corticotropic tumor cells, levels of PAM mRNA and pro-ACTH/endorphin mRNA were regulated in parallel by glucocorticoids and CRF.
Eipper
B A
BA
Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
Park
L P
LP
Dickerson
I M
IM
Keutmann
H T
HT
Thiele
E A
EA
Rodriguez
H
H
Schofield
P R
PR
Mains
R E
RE
D000577:Amides / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D000917:Antibody Formation / Q000502:physiology; D001483:Base Sequence; D015152:Blotting, Northern; D002417:Cattle; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004792:Enzyme Precursors / Q000737:chemistry*; D006899:Mixed Function Oxygenases / Q000737:chemistry* / Q000235:genetics / Q000502:physiology; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes; D010452:Peptide Biosynthesis; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000276:immunology
D000577:Amides; D004792:Enzyme Precursors; D009097:Multienzyme Complexes; D011993:Recombinant Fusion Proteins; D004247:DNA; D006899:Mixed Function Oxygenases; C035189:peptidylglycine monooxygenase