Suppression of polyclonal B cell activation by IgG-binding factors. Requirement for T cells.
IgG-binding factors (IgG-BF) prepared from cell-free supernatant of human peripheral blood mononuclear cells interfere with the polyclonal activation of peripheral B cells by decreasing the numbers of IgG-containing cells and Ig plaque-forming cells. Using Nocardia opaca delipidated cell mitogen (NDCM), a T helper cell-independent polyclonal B cell activator, it was found that the suppressive effect of IgG-BF was no longer demonstrable after removal of T cells. In pokeweed mitogen-stimulated cultures, the suppression by IgG-BF required the presence of radiosensitive T cells. Selective depletion of OKT4+ or OKT8+ subsets in NDCM-stimulated cultures showed that IgG-BF required the presence of OKT4+ lymphocytes to induce suppression. It is concluded that the effect of human IgG-BF was mediated by one or several subsets of T cells.
Lê thi Bich-Thuy
Revillard
J P
JP
D000911:Antibodies, Monoclonal; D001402:B-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D007108:Immune Tolerance; D007070:Immunoglobulin A / Q000096:biosynthesis; D007126:Immunoglobulin Allotypes / Q000502:physiology; D007074:Immunoglobulin G / Q000096:biosynthesis; D008213:Lymphocyte Activation; D008222:Lymphokines / Q000502:physiology*; D010641:Phenotype; D029584:Prostatic Secretory Proteins; D013601:T-Lymphocytes / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology
D000911:Antibodies, Monoclonal; D007070:Immunoglobulin A; D007126:Immunoglobulin Allotypes; D007074:Immunoglobulin G; D008222:Lymphokines; D029584:Prostatic Secretory Proteins; C043076:beta-microseminoprotein; C013059:immunoglobulin-binding factors