Lymphocyte subpopulations do not alter during blood storage at 4 degrees C.
Recent articles have suggested that lymphocyte subpopulations, as determined by monoclonal antibodies, alter during overnight storage of whole blood at 4 degrees C. In contrast, we report here that the expression of lymphocyte subpopulation markers does not alter, but that aberrant results may be obtained due to a large amount of contaminating granulocytes in the lymphocyte preparation, thus indicating that the 'apparent loss' of lymphocyte markers is a technical rather than a biological problem.
Van Lambalgen
R
R
Van Meurs
G J
GJ
D000911:Antibodies, Monoclonal; D001793:Blood Preservation; D002469:Cell Separation; D005434:Flow Cytometry; D006801:Humans; D008214:Lymphocytes / Q000145:classification*; D006377:T-Lymphocytes, Helper-Inducer / Q000166:cytology; D050378:T-Lymphocytes, Regulatory / Q000166:cytology; D013696:Temperature
D000911:Antibodies, Monoclonal