Fusion between human and Drosophila melanogaster cells induced by polyethylene glycol.
Polyethylene glycol was used to induce interspecific somatic cell fusion between human fibroblasts (stock F6) and Drosophila melanogaster cells from established cell lines (Cl 82 and 11 P102), characterized by different ploidy levels. The present investigation defines some parameters for Drosophila cell fusion and interspecific fusion between Drosophila and human cells. The cytological analysis provided evidence of spontaneous as well as induced human-Drosophila heterokaryon formation. The presence in the same cell of two types of nuclei, distinguishable because of their different size and morphology, was confirmed autoradiographically by 3H-thymidine pre-labelling of Drosophila cells. Furthermore, the retained DNA synthetic activity and some examples of mitotic figures of both types of nuclei in the heterokaryons indicate the viability of the fused cells.
Halfer
C
C
Dipartimento di Genetica e di Biologia dei Microorganismi, Università di Milano, Italy.
Del Pin
D
D
Dell'Oro
A
A
D000818:Animals; D001345:Autoradiography; D002455:Cell Division; D002459:Cell Fusion / Q000187:drug effects*; D004247:DNA / Q000096:biosynthesis; D004331:Drosophila melanogaster / Q000235:genetics; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D006801:Humans; D011092:Polyethylene Glycols / Q000494:pharmacology*
D011092:Polyethylene Glycols; D004247:DNA