Tandem separation of labelled human blood platelet membrane glycoproteins by anion-exchange and gel fast protein liquid chromatography.
Studies were made of the separation of surface-labelled platelet glycoproteins, solubilized in a non-ionic detergent (Berol 185), on a Mono Q anion-exchange column, coupled to a Superose 12 gel fast protein liquid chromatographic column. Peaks eluted from the anion-exchange and gel columns were subjected to electrophoresis on sodium dodecyl sulphate-polyacrylamide gels in the presence (non-reducing conditions) or absence (reducing conditions) of disulphide bridges. Labelled electrophoresed glycoprotein bands were rendered visible by fluorography or indirect autoradiography. Platelet membrane glycoproteins Ib, IIb, IIIa, IIIb, V and IX were identified by their apparent molecular weights and their surface labelling characteristics. It was concluded that tandem chromatography can be used to separate platelet membrane proteins and glycoproteins rapidly and with good resolution.
McGregor
J L
JL
Clezardin
P
P
Manach
M
M
Gronlund
S
S
Dechavanne
M
M
D001792:Blood Platelets / Q000032:analysis*; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D002853:Chromatography, Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000302:isolation & purification*; D006801:Humans; D008565:Membrane Proteins / Q000302:isolation & purification*; D010980:Platelet Membrane Glycoproteins; D012967:Sodium Dodecyl Sulfate
D006023:Glycoproteins; D008565:Membrane Proteins; D010980:Platelet Membrane Glycoproteins; D012967:Sodium Dodecyl Sulfate