[Effect of metabolic inhibitors on formed novocaine and neutral red segregation zones in frog erythrocytes].
Effects of some metabolic inhibitors, as well as of biologically active compounds (diakarb, ethidium bromide and a phenanthridine alkaloid sanguinarine) on the formed novocaine and neutral red segregation zones were studied. The volume of granules diminished under the influence of a glycolytic inhibitor iodoacetate, uncouplers of oxidative phosphorylation (2,4-dinitrophenol and carbonyl cyanide trifluoromethoxyphenylhydrozone), and respiratory inhibitors (antimycin A and rotenone), as well as under the influence of cycloheximide - an inhibitor of protein synthesis. Diakarb, ethidium bromide or sanguinarine also provoked a regression of the segregation zones. It has been found that all these compounds are inhibitors of ATPase activity of the isolated segregation zones. A possible mechanism of volume decreasing in segregation zones under the influence of both the metabolic inhibitors and diakarb, ethidium bromide and sanguinarine is discussed.
Veselkina
M N
MN
Bulychev
A G
AG
Braun
A D
AD
D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors; D000818:Animals; D000963:Antimetabolites / Q000494:pharmacology*; D003594:Cytoplasmic Granules / Q000187:drug effects* / Q000378:metabolism; D004912:Erythrocytes / Q000187:drug effects* / Q000378:metabolism; D009499:Neutral Red / Q000097:blood*; D010085:Oxidative Phosphorylation / Q000187:drug effects; D010619:Phenazines / Q000097:blood*; D011343:Procaine / Q000097:blood*; D011896:Rana temporaria; D013997:Time Factors
D000963:Antimetabolites; D010619:Phenazines; D009499:Neutral Red; D011343:Procaine; D000251:Adenosine Triphosphatases
Vliianie ingibitorov metabolizma na sformirovannye zony segregatsii novokaina i neĭtral'nogo krasnogo v éritrotsitakh liagushki.