CD4 cytotoxic T lymphocyte differentiation.
In vitro allostimulated CD4+ human lymphocytes were cloned by micromanipulation and expanded for a short time in IL-2 conditioned medium. In the present study we observed that proliferative noncytotoxic cloned cells were able to acquire the specific cytolytic activity under some modification of the cloned cells restimulation cycle. We demonstrated that rIFN-alpha and -gamma are the agents responsible for the acquisition of specific lytic activity.
Bensussan
A
A
Unité INSERM U 93, Hôpital Saint-Louis, Paris, France.
D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology*; D015139:Blotting, Southern; D002455:Cell Division; D002478:Cells, Cultured; D002999:Clone Cells; D003602:Cytotoxicity, Immunologic; D006801:Humans; D008213:Lymphocyte Activation; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology / Q000276:immunology*
D000945:Antigens, Differentiation, T-Lymphocyte