Clonal nature of murine hemopoietic blast cell colonies.
Murine hemopoietic blast cell colonies obtained from spleen cells of 5-fluorouracil (5-FU)-treated mice give rise to many multilineage colonies including granulocyte - erythrocyte - macrophage - megakaryocyte (GEMM) colonies in secondary cultures. Progenitor cells for blast cell colonies are considered to be more primitive than colony forming units (CFU)-GEMM. To determine whether they are clonal, we examined the phosphoglycerate kinase-1 (PGK-1) isozyme type of colonies originally grown from spleen cells of 5-FU-treated mice which had PGK-1 isozyme mosaicism. PGK assays of whole secondary colonies derived from one blast cell colony showed that they were either of type A or type B but not both. These results suggest that murine hemopoietic blast cell colonies are clonal.
Fujimori
Y
Y
Second Department of Internal Medicine, Hyogo College of Medicine, Japan.
Hara
H
H
Taguchi
T
T
Kitamura
Y
Y
Nagai
K
K
D000818:Animals; D002999:Clone Cells / Q000166:cytology*; D003114:Colony-Forming Units Assay; D005260:Female; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D051379:Mice; D009030:Mosaicism; D010735:Phosphoglycerate Kinase; D013154:Spleen / Q000166:cytology
D010735:Phosphoglycerate Kinase