Transforming growth factor beta regulates the metabolism of proteoglycans in bovine cartilage organ cultures.
The effect of transforming growth factor beta (TGF-beta) has been studied in a bovine articular cartilage organ culture. The peptide stimulates synthesis of proteoglycans in a dose-dependent manner, reaching saturation at 10 ng/ml. This dose gave an approximate 7-fold increase in synthesis over basal controls. In addition, the peptide decreased the rates of catabolism of proteoglycans with an approximately 2-fold maximal effect seen at 5 ng/ml. At the latter concentration, TGF-beta prevented the 4-fold loss of proteoglycans which occurred in cultures maintained under basal conditions over the course of 3 weeks. There was no increase in cell (DNA) content of the cartilage explants under these conditions of TGF-beta treatment, and the net collagen content of the explants remained constant.
Morales
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Bone Research Branch, National Institute of Dental Research, Bethesda, Maryland 20892.
Roberts
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D000818:Animals; D002358:Cartilage, Articular / Q000378:metabolism*; D002417:Cattle; D003094:Collagen / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D006025:Glycosaminoglycans / Q000378:metabolism; D009924:Organ Culture Techniques; D010455:Peptides / Q000378:metabolism*; D011509:Proteoglycans / Q000378:metabolism*; D015291:Transforming Growth Factors
D006025:Glycosaminoglycans; D010455:Peptides; D011509:Proteoglycans; D015291:Transforming Growth Factors; D003094:Collagen