Mannose-mediated targeted immunoadjuvant action of liposomes.
The effect of the ligand mannosylated albumin, covalently coupled to the surface of tetanus toxoid-containing dehydration-rehydration vesicles (DRV), on their adjuvanticity was investigated. Toxoid-containing DRV coated with mannosylated albumin bound in vitro to mouse (BALB/c) peritoneal macrophages selectively and to a greater extent than non-mannosylated DRV. ELISA assays of anti-toxoid IgG1 and IgG2b in the sera of BALB/c mice immunized with various toxoid-containing DRV preparations suggest that mannosylated liposomes are superior in immunoadjuvant action to conventional ones. Such targeted adjuvanticity appears to be influenced by the number of ligand molecules available on the surface of liposomes rather than the number of mannose residues on albumin.
Garcon
N
N
Medical Research Council Group, Royal Free Hospital School of Medicine, London, U.K.
Gregoriadis
G
G
Taylor
M
M
Summerfield
J
J
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D008081:Liposomes / Q000276:immunology* / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism; D008358:Mannose / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D013745:Tetanus Toxoid / Q000276:immunology
D000276:Adjuvants, Immunologic; D000907:Antibodies, Bacterial; D008081:Liposomes; D013745:Tetanus Toxoid; D008358:Mannose