Immunochemical approach for the characterization of DNA damages induced by chemical carcinogens.
Mouse monoclonal antibody was elicited with 4-nitroquinoline 1-oxide (4NQO) modified poly(dG-dC).poly(dG-dC) and was characterized using enzyme-linked immunosorbent assay and radioimmunoassay. The antibody reacted specifically for 4NQO-poly(dG-dC).poly(dG-dC) but not for 4NQO modified DNA and synthetic polynucleotides such as poly(dG).poly(dC). The antibody crossreacted slightly with brominated or N-acetoxy-2-acetylaminofluorene modified poly(dG-dC).poly(dG-dC) known to adopt Z-conformation. The antibody may recognize unique conformational change in poly(dG-dC).poly(dG-dC) modified by 4NQO. The antibody should be useful for the detection of conformational change in DNA induced by chemical carcinogens.
Tada
M
M
Laboratory of Biochemistry, Aichi Cancer Center, Research Institute, Osaka, Japan.
Morita
T
T
D015112:4-Nitroquinoline-1-oxide; D000911:Antibodies, Monoclonal; D002273:Carcinogens; D004247:DNA / Q000032:analysis* / Q000276:immunology; D004249:DNA Damage; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D011089:Polydeoxyribonucleotides / Q000032:analysis / Q000276:immunology; D011863:Radioimmunoassay / Q000379:methods
D000911:Antibodies, Monoclonal; D002273:Carcinogens; D011089:Polydeoxyribonucleotides; C019530:poly(dC-dG); D015112:4-Nitroquinoline-1-oxide; D004247:DNA