Isolation of T lymphocyte subsets from peripheral blood using monoclonal antilymphocyte antibodies.
Monoclonal antisera against helper (serum OKT4) and suppressor (serum OKT8) T cells were employed in a complement-dependent lymphocytotoxicity reaction to isolate helper and suppressor T cells from peripheral blood mononuclear cells. When prepared by this method, helper and suppressor T cells had a purity of 67.8 percent and 78.8 percent, respectively. Approximately 56 percent of viable suppressor and 33 percent of viable helper T cells were lost during the procedure.
Patel
R
R
Soriano
A
A
Loeb
L
L
Ghoneum
M
M
Najera
D
D
D000911:Antibodies, Monoclonal; D006801:Humans; D013601:T-Lymphocytes / Q000145:classification*; D006377:T-Lymphocytes, Helper-Inducer; D050378:T-Lymphocytes, Regulatory
D000911:Antibodies, Monoclonal