title: An ELISA for PGE2 utilizing monoclonal antibody.
abstract: An ELISA for PGE2 has been developed which is sensitive to concentrations of 0.5 to 20.0 ng PGE2/ml. Mouse monoclonal anti-PGE2 ascites is utilized in a binding competition between the test sample and an adsorbed conjugate of PGE2-BSA. The antibody which remains bound to the solid phase is quantitated colorimetrically by incubation with alkaline phosphatase-conjugated goat anti-mouse IgG followed by incubation with p-nitro-phenylphosphate. PGE1, PGA1, PGA2, PGB2, 6-keto-PGF1 alpha, PGF2 alpha, 13,14-dihydro-15-keto-PGE2, thromboxane B2 and arachidonic acid showed minimal cross-reactivity with the anti-PGE2. The PGE2 ELISA permits the quantitative analysis of large numbers of samples at a fraction of the cost and time required to process a commercial RIA kit. When linked to the appropriate computer software, data collection and analysis can be performed in less than 10 minutes per 96-well plate. Furthermore, the use of an ELISA system eliminates the radioactive and toxic chemical waste generated by RIA methods.
authors.affiliation: Ayerst Laboratories Research, Inc., Department of Immunology, Princeton, NJ 08543.
authors.forename: R G
authors.initials: RG
authors.lastname: Neuman
authors.forename: J H
authors.initials: JH
authors.lastname: Korn
authors.forename: E T
authors.initials: ET
authors.lastname: Lally
authors.forename: D D
authors.initials: DD
authors.lastname: Wood
authors.forename: E S
authors.initials: ES
authors.lastname: Kimball
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D003429:Cross Reactions; D003470:Culture Media / Q000032:analysis; D015232:Dinoprostone; D004797:Enzyme-Linked Immunosorbent Assay; D011458:Prostaglandins E / Q000032:analysis* / Q000276:immunology; D011459:Prostaglandins E, Synthetic / Q000032:analysis* / Q000276:immunology; D012997:Solvents; D013223:Statistics as Topic; D013582:Synovial Fluid / Q000166:cytology; D014556:Urine / Q000032:analysis
chemical_list: D000911:Antibodies, Monoclonal; D003470:Culture Media; D011458:Prostaglandins E; D011459:Prostaglandins E, Synthetic; D012997:Solvents; D015232:Dinoprostone