title: Identification of aldosterone secretion inhibitory factor in bovine adrenal medulla.
abstract: We report the first demonstration of an Aldosterone Secretion Inhibitory Factor (ASIF) in acid extracts of bovine adrenal medulla. Following separation from catecholamines and enkephalins, this factor leads to an 80% inhibition of PGE1-stimulated secretion of aldosterone from bovine adrenal zona glomerulosa. ASIF is retained on cation exchange gels and behaves as a small 5K-dalton peptide on Sephadex G-50. This factor cross-reacts in a radio-receptor assay for [125I] atrial natriuretic factor (ANF). ASIF is distinct from all neuropeptides formerly detected in the adrenal medulla, e.g. somatostatin, enkephalin, neuropeptide Y, dynorphin, neurotensin. In the adrenal gland, this ANF-like factor is predominantly found in the medulla (4 pmol/mg protein), with only trace amounts in the cortex (0.1 pmol/mg protein). ASIF might perhaps correspond to the endogenous ligand for the receptor sites that we have previously identified with [125I]ANF in bovine adrenal cortex and could contribute to the formerly reported attenuating influence of the adrenal medulla on mineralocorticoid production.
authors.forename: A
authors.initials: A
authors.lastname: De Léan
authors.forename: H
authors.initials: H
authors.lastname: Ong
authors.forename: N
authors.initials: N
authors.lastname: McNicoll
authors.forename: R
authors.initials: R
authors.lastname: Racz
authors.forename: J
authors.initials: J
authors.lastname: Gutkowska
authors.forename: M
authors.initials: M
authors.lastname: Cantin
mesh_terms: D000313:Adrenal Medulla / Q000032:analysis* / Q000378:metabolism; D000450:Aldosterone / Q000378:metabolism*; D000818:Animals; D009320:Atrial Natriuretic Factor; D002417:Cattle; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D003429:Cross Reactions; D000451:Mineralocorticoid Receptor Antagonists / Q000032:analysis* / Q000494:pharmacology; D009124:Muscle Proteins / Q000032:analysis; D011458:Prostaglandins E / Q000494:pharmacology; D011869:Radioligand Assay; D051381:Rats
chemical_list: D000451:Mineralocorticoid Receptor Antagonists; D009124:Muscle Proteins; D011458:Prostaglandins E; D000450:Aldosterone; D009320:Atrial Natriuretic Factor