Use of a fluorescent radiolabeled triacylglycerol as a substrate for lipoprotein lipase and hepatic triglyceride lipase.
A fluorescent radiolabeled triacylglycerol has been synthesized by using a fluorescent fatty acid (pyrene decanoic acid) and a radiolabeled oleic acid. This analog of the natural substrate, 1(3)pyrene decanoic-2,3 (1,2)-dioleoyl-sn-glycerol, has been tested as substrate for determining lipoprotein lipase and hepatic triacylglycerol lipase activities in post-heparin plasma. Optimal conditions for the determination of the two post-heparin plasma lipases were similar to those using radiolabeled triolein. Using this substrate, both post-heparin lipases exhibited their characteristic properties (pH optimum and effect of inhibitors) and attacked external ester bonds (1 or 3) containing pyrene decanoic and oleic acids at a similar rate.
Dousset
N
N
INSERM Unite 101, Hôpital Purpan, Toulouse, France.
Negre
A
A
Salvayre
R
R
Rogalle
P
P
Dang
Q Q
QQ
Douste-Blazy
L
L
D000818:Animals; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008049:Lipase / Q000037:antagonists & inhibitors / Q000097:blood*; D008071:Lipoprotein Lipase / Q000037:antagonists & inhibitors / Q000097:blood*; D008099:Liver / Q000201:enzymology*; D011817:Rabbits; D012965:Sodium Chloride / Q000494:pharmacology; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D013050:Spectrometry, Fluorescence; D014280:Triglycerides; D014316:Tritium
D014280:Triglycerides; D014316:Tritium; D012967:Sodium Dodecyl Sulfate; D012965:Sodium Chloride; C046439:1-(10-pyrenedecanoyl)-2,3-dioleoylglycerol; D008049:Lipase; D008071:Lipoprotein Lipase