title: Acquired ability of Staphylococcus aureus to produce toxic shock-associated protein and resulting illness in a rabbit model.
abstract: Staphylococcus aureus from patients with toxic shock syndrome (TSS) produce TSS toxin 1. We transferred, by a bacteriophage, the ability to produce TSS toxin 1 from a TSS toxin 1-positive to a TSS toxin 1-negative strain of S. aureus. This recombinant strain produced TSS toxin 1 as confirmed by isoelectric focusing, immunodiffusion, radioimmunoassay, and autoradiography. The recombinant produced TSS-like illness in rabbits, and was significantly (P less than 0.001) more lethal than the recipient strain. Both strains produced fever and diarrhea, but, in addition, rabbits challenged with the recombinant also developed lowered blood pressure (P = 0.002), conjunctival hyperemia, erythroderma, and respiratory distress. Histopathological findings in rabbits challenged with the recombinant strain were remarkably similar to those described for humans with TSS, e.g., erythrophagocytosis, liver "triaditis," and vasodilatation. This study demonstrates that this protein may contribute to the pathogenesis of the TSS.
authors.forename: J K
authors.initials: JK
authors.lastname: Rasheed
authors.forename: R J
authors.initials: RJ
authors.lastname: Arko
authors.forename: J C
authors.initials: JC
authors.lastname: Feeley
authors.forename: F W
authors.initials: FW
authors.lastname: Chandler
authors.forename: C
authors.initials: C
authors.lastname: Thornsberry
authors.forename: R J
authors.initials: RJ
authors.lastname: Gibson
authors.forename: M L
authors.initials: ML
authors.lastname: Cohen
authors.forename: C D
authors.initials: CD
authors.lastname: Jeffries
authors.forename: C V
authors.initials: CV
authors.lastname: Broome
mesh_terms: D000818:Animals; D001427:Bacterial Toxins; D004195:Disease Models, Animal; D004768:Enterotoxins / Q000235:genetics / Q000633:toxicity*; D011817:Rabbits; D012772:Shock, Septic / Q000382:microbiology* / Q000473:pathology; D013204:Staphylococcus Phages / Q000235:genetics; D013211:Staphylococcus aureus / Q000235:genetics / Q000472:pathogenicity*; D018089:Superantigens
chemical_list: D001427:Bacterial Toxins; D004768:Enterotoxins; D018089:Superantigens; C030107:enterotoxin F, Staphylococcal