Enhancement of acyl coenzyme A:retinol acyltransferase in rat liver and mammary tumor tissue by retinyl acetate and its competitive inhibition by N-(4-hydroxyphenyl) retinamide.
Retinol esterification by microsomal acyl coenzyme A:retinol acyltransferase was quantified in rat mammary tumor and liver tissue. Acyltransferase activity in the livers of mammary tumor-bearing rats was 40% of that in normal animals. In response to daily oral doses of 2 mg retinyl acetate for 18-19 days, activity increased 2.8-fold in transplanted rat mammary tumors, 4.1-fold in the livers of tumor-bearing rats, and 1.5-fold in the livers of normal rats. The in vitro esterification of retinol was competitively inhibited by all-trans-N-(4-hydroxyphenyl) retinamide (Ki = 154 microM).
Ball
M D
MD
Furr
H C
HC
Olson
J A
JA
D000217:Acyltransferases / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D004224:Diterpenes; D005260:Female; D017313:Fenretinide; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008325:Mammary Neoplasms, Experimental / Q000201:enzymology*; D008862:Microsomes, Liver / Q000201:enzymology; D051381:Rats; D011916:Rats, Inbred F344; D051142:Retinol O-Fatty-Acyltransferase; D000084562:Retinyl Esters; D014212:Tretinoin / Q000031:analogs & derivatives* / Q000494:pharmacology; D014801:Vitamin A / Q000031:analogs & derivatives* / Q000494:pharmacology
D004224:Diterpenes; D000084562:Retinyl Esters; D014801:Vitamin A; D017313:Fenretinide; C009166:retinol acetate; D014212:Tretinoin; D000217:Acyltransferases; D051142:Retinol O-Fatty-Acyltransferase