Thymidine kinase in human leukemia. Expression of the lymphoblastic isoenzyme in three patients with acute myelocytic leukemia.
The dominating thymidine kinase activity in mononuclear white blood cells from three patients with untreated acute myelocytic leukemia (AML) was compared with TK 1 from phytohemagglutinin-stimulated and TK 2 from unstimulated, normal lymphocytes. The enzyme activity in the AML cells and the stimulated lymphocytes was found to be in the same range. Regarding the combined thymidine and dTTP kinetics, the enzymes from the three AML patients resembled TK 1, but the ATP kinetics were different and the molecular weights were lower, as previously found for thymidine kinases from other leukemic cells. Therefore, the designation TK-1-onc is suggested for the thymidine kinases from the AML cells.
Munch-Petersen
B
B
Department of Biochemistry C, University of Copenhagen, Denmark.
Tyrsted
G
G
D000255:Adenosine Triphosphate / Q000378:metabolism; D006801:Humans; D007527:Isoenzymes / Q000032:analysis*; D007700:Kinetics; D015470:Leukemia, Myeloid, Acute / Q000201:enzymology*; D008213:Lymphocyte Activation / Q000187:drug effects; D009363:Neoplasm Proteins / Q000032:analysis*; D010835:Phytohemagglutinins / Q000494:pharmacology; D013937:Thymidine Kinase / Q000032:analysis*; D013942:Thymine Nucleotides / Q000378:metabolism
D007527:Isoenzymes; D009363:Neoplasm Proteins; D010835:Phytohemagglutinins; D013942:Thymine Nucleotides; D000255:Adenosine Triphosphate; D013937:Thymidine Kinase; C024157:thymidine 5'-triphosphate