Cytidine 5'-monophospho-N-acetylneuraminic acid or a related compound is the low Mr factor from human red blood cells which induces gonococcal resistance to killing by human serum.
A low-Mr factor which induces gonococcal resistance to complement-mediated serum killing has been partially purified from lysates of mixed red and buffy coat cells from human blood. The lysates were dialysed against Tris buffer for 24 h at 25 degrees C with the diffusate being continuously recycled through a column of QAE-Sephadex A25. After elution in an NaCl gradient, the active fractions were both desalted and further purified on Sephadex G10. A second fractionation on QAE-Sephadex A25 and desalting with Sephadex G10 preceded further purification by repeated high-pressure liquid chromatography (HPLC) using a DEAE anion exchange column and desalting with Sephadex G10. Less than 500 micrograms of material showing one peak in HPLC was obtained from 1 litre of blood. After NMR had indicated the possible presence of pyrimidine nucleotide, carbohydrate and N-acetyl groups, nanogram quantities of a commercial preparation of cytidine 5'-monophospho-N-acetylneuraminic acid (CMP-NANA) were shown to induce gonococci to serum resistance. The synthetic CMP-NANA also co-eluted with the preparation from blood cells in HPLC, and the two materials were indistinguishable in their patterns of acid and heat lability. Furthermore, the resistance-inducing activity of both materials was inhibited by cytidine monophosphate, which is known to inhibit sialylation reactions by CMP-NANA. It appears therefore that the resistance-inducing factor is CMP-NANA or a closely related compound. If the factor is CMP-NANA, biological activities indicated that the cell lysate from 1 litre of blood contained about 40 micrograms, and the most purified preparation contained only about 1%. With this minute amount in a mixture, the presence of CMP-NANA or a closely related analogue could not be established unequivocally by NMR.
Nairn
C A
CA
Department of Microbiology, University of Birmingham, UK.
Cole
J A
JA
Patel
P V
PV
Parsons
N J
NJ
Fox
J E
JE
Smith
H
H
D001770:Blood Bactericidal Activity; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D003569:Cytidine Monophosphate N-Acetylneuraminic Acid / Q000097:blood / Q000276:immunology* / Q000302:isolation & purification; D004912:Erythrocytes / Q000276:immunology*; D006801:Humans; D007108:Immune Tolerance; D008970:Molecular Weight; D009344:Neisseria gonorrhoeae / Q000276:immunology*; D012794:Sialic Acids / Q000276:immunology*
D012794:Sialic Acids; D003569:Cytidine Monophosphate N-Acetylneuraminic Acid