Adenosine deaminase gene expression is regulated posttranscriptionally in the nucleus.
The housekeeping enzyme adenosine deaminase (ADA) shows a large variation in tissue-specific expression ranging from 1 Iu in red blood cells to 880 Iu in thymocytes. We investigated the acute lymphocytic leukemic cell line Molt-4 (660 Iu ADA/g protein) and the promyelocytic cell line HL-60 (38 Iu ADA/g protein) as a model system to determine the levels at which the tissue-specific expression of ADA is regulated. From our results it can be concluded that the almost 20-fold difference in ADA expression between Molt-4 and HL-60 is the result of differences in the post-transcriptional processing and/or stability of ADA pre-mRNA within the nucleus.
Berkvens
T M
TM
Department of Medical Biochemistry, Sylvius Laboratories, Leiden, The Netherlands.
Schoute
F
F
van Ormondt
H
H
Meera Khan
P
P
van der Eb
A J
AJ
D000243:Adenosine Deaminase / Q000235:genetics*; D002467:Cell Nucleus / Q000378:metabolism*; D004790:Enzyme Induction; D006801:Humans; D007945:Leukemia, Lymphoid / Q000473:pathology; D015470:Leukemia, Myeloid, Acute / Q000473:pathology; D009700:Nucleoside Deaminases / Q000235:genetics*; D012322:RNA Precursors / Q000378:metabolism*; D012323:RNA Processing, Post-Transcriptional; D012334:RNA, Neoplasm / Q000378:metabolism; D014407:Tumor Cells, Cultured / Q000378:metabolism
D012322:RNA Precursors; D012334:RNA, Neoplasm; D009700:Nucleoside Deaminases; D000243:Adenosine Deaminase