A (2'-5')An-dependent endonuclease: tissue distribution in BALB/c mice and the effects of IFN-beta treatment and anti-IFN-alpha/beta immunoglobulin on the levels of the enzyme.
A (2'-5')oligoadenylate-dependent endoribonuclease (RNase L) is an important mediator of the IFN-induced antiviral state. RNase L has been found in several different murine tissues at levels comparable to those previously observed in cultured cells. A basal level of RNase L is present in all tissues assayed. The levels of RNase L in tissues are not significantly increased in mice following treatment with interferon-beta (IFN-beta). The amount of RNase L is, however, decreased in spleen when mice are injected with rabbit antimouse IFN-alpha/beta immunoglobulin. These data suggest that relatively high levels of RNase L may be maintained in tissues by endogenous IFN-alpha/beta production.
Floyd-Smith
G
G
Department of Zoology, Arizona State University, Tempe 85287.
Denton
J S
JS
D000818:Animals; D000906:Antibodies / Q000276:immunology; D004722:Endoribonucleases / Q000096:biosynthesis*; D004790:Enzyme Induction; D007370:Interferon Type I / Q000276:immunology / Q000494:pharmacology*; D007422:Intestines / Q000201:enzymology; D007668:Kidney / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D008168:Lung / Q000201:enzymology; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000201:enzymology
D000906:Antibodies; D007370:Interferon Type I; D004722:Endoribonucleases; C031956:2-5A-dependent ribonuclease