Jacalin, the human IgA1 and IgD precipitating lectin, also binds IgA2 of both allotypes.
The lectin jacalin from jackfruit seeds shows a human IgA-subclass specificity by gel precipitation and Western blotting. However, its reactivity with IgA2 is a matter of controversy. We further studied the immunoglobulin isotype specificity of jacalin by affinity chromatography with myeloma sera and by inhibition of jacalin binding to solid-phase IgA1 by purified monoclonal immunoglobulins. The lectin proved to bind IgA2 of both allotypes with a lower apparent affinity than for IgA1 and IgD.
Aucouturier
P
P
Laboratory of Immunology and Immunopathology, CNRS UA 1172, Poitiers, France.
Duarte
F
F
Mihaesco
E
E
Pineau
N
N
Preud'homme
J L
JL
D000911:Antibodies, Monoclonal; D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D015153:Blotting, Western; D002352:Carrier Proteins / Q000302:isolation & purification; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D006801:Humans; D007122:Immunoelectrophoresis; D007070:Immunoglobulin A / Q000302:isolation & purification / Q000378:metabolism*; D007126:Immunoglobulin Allotypes / Q000302:isolation & purification / Q000378:metabolism*; D007072:Immunoglobulin D / Q000302:isolation & purification / Q000378:metabolism*; D037102:Lectins; D037121:Plant Lectins; D011233:Precipitin Tests
D000911:Antibodies, Monoclonal; D002352:Carrier Proteins; D007070:Immunoglobulin A; D007126:Immunoglobulin Allotypes; D007072:Immunoglobulin D; D037102:Lectins; D037121:Plant Lectins; C043696:jacalin