title: Changes in skeletal muscle gene transcription induced by chronic stimulation.
abstract: The mRNA products of four genes, carbonic anhydrase III (CAIII), fast myosin heavy chain (MHCf), actin and glyceraldehyde-3-phosphate dehydrogenase (GAPDH), were assayed by Northern and slot-blot analysis in rabbit tibialis anterior and extensor digitorum longus muscles that were subjected to electrical stimulation for periods of up to 21 days. Marked changes in mRNA levels were seen for all four genes. The mRNA for CAIII, which is specific for type 1 fibers, rose significantly, whereas the MHCf mRNA fell markedly in the stimulated muscles. Changes in GAPDH mRNA were consistent with a reduced dependence on anaerobic glycolysis as an energy source. Actin mRNA levels were noticeably depressed in the early stages of stimulation. Thus for several classes of muscle protein, the response to chronic low-frequency stimulation appears to involve changes at the level of gene transcription.
authors.affiliation: MRC Human Biochemical Genetics Unit, Galton Laboratory, University College London, UK.
authors.forename: C
authors.initials: C
authors.lastname: Brownson
authors.forename: H
authors.initials: H
authors.lastname: Isenberg
authors.forename: W
authors.initials: W
authors.lastname: Brown
authors.forename: S
authors.initials: S
authors.lastname: Salmons
authors.forename: Y
authors.initials: Y
authors.lastname: Edwards
mesh_terms: D000199:Actins / Q000235:genetics*; D000818:Animals; D002256:Carbonic Anhydrases / Q000235:genetics*; D004558:Electric Stimulation; D005260:Female; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics*; D066298:In Vitro Techniques; D008297:Male; D015335:Molecular Probes; D009119:Muscle Contraction; D009132:Muscles / Q000378:metabolism*; D009218:Myosins / Q000235:genetics*; D011446:Prospective Studies; D012333:RNA, Messenger / Q000032:analysis; D011817:Rabbits; D014158:Transcription, Genetic
chemical_list: D000199:Actins; D015335:Molecular Probes; D012333:RNA, Messenger; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D009218:Myosins; D002256:Carbonic Anhydrases