A microtiter cell-culture assay for the determination of anti-human immunodeficiency virus neutralizing antibody activity.
A microtiter cell-culture assay is described for measuring neutralizing antibody activity directed against the human immunodeficiency virus (HIV). The assay relies upon inhibition of HIV-mediated cell killing of infected MT-4 lymphoid cells. The assay exhibits comparable sensitivity to two other methods used for such measurements, is relatively rapid, may be adapted for screening large numbers of samples and involves minimal handling of infectious virus.
Robertson
G A
GA
Department of Virus and Cell Biology, Merck, Sharp and Dohme Research Laboratories, West Point, Pennsylvania 19486.
Kostek
B M
BM
Schleif
W A
WA
Lewis
J A
JA
Emini
E A
EA
D000914:Antibodies, Viral / Q000032:analysis; D002460:Cell Line; D002470:Cell Survival; D004396:Coloring Agents; D006678:HIV / Q000302:isolation & purification*; D015483:HIV Antibodies; D009500:Neutralization Tests / Q000379:methods; D013778:Tetrazolium Salts; D013844:Thiazoles
D000914:Antibodies, Viral; D004396:Coloring Agents; D015483:HIV Antibodies; D013778:Tetrazolium Salts; D013844:Thiazoles; C022616:thiazolyl blue