Identification and partial characterization of a new pancreatic cancer-related serum glycoprotein by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and lectin blotting.
The combination of sodium dodecyl sulfate-polyacrylamide gel electrophoresis and peanut agglutinin lectin blotting has been used to identify a pancreatic cancer-related glycoprotein in serum. Its high molecular weight and its lectin-binding characteristics suggest that it probably is a mucin. Comparison with immunoblotting with Ca 19-9 antibody suggests that the epitopes for Ca 19-9 and peanut agglutinin may be variably expressed on different sites of the same mucin. The peanut agglutinin-binding glycoprotein was demonstrated in 12 of 34 pancreatic cancer sera, including four with normal Ca 19-9 assay, and none of 96 control sera, including sera from patients with obstructive jaundice and other cancers. These preliminary results suggest that it may become a useful addition to the current serum markers for pancreatic cancer. The technique of sodium dodecyl sulfate-polyacrylamide gel electrophoresis and lectin blotting may well prove valuable in the search for other cancer-related glycoprotein markers.
Ching
C K
CK
University Department of Medicine, Liverpool, United Kingdom.
Rhodes
J M
JM
D000951:Antigens, Neoplasm / Q000032:analysis; D015295:Antigens, Tumor-Associated, Carbohydrate; D014408:Biomarkers, Tumor / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000032:analysis* / Q000097:blood; D006801:Humans; D007118:Immunoassay; D037102:Lectins / Q000378:metabolism*; D008970:Molecular Weight; D009363:Neoplasm Proteins / Q000032:analysis*; D010190:Pancreatic Neoplasms / Q000175:diagnosis*; D019887:Peanut Agglutinin; D012967:Sodium Dodecyl Sulfate
D000951:Antigens, Neoplasm; D015295:Antigens, Tumor-Associated, Carbohydrate; D014408:Biomarkers, Tumor; D006023:Glycoproteins; D037102:Lectins; D009363:Neoplasm Proteins; D019887:Peanut Agglutinin; D012967:Sodium Dodecyl Sulfate