[Ultrastructural localization of mRNA coding for oxytocin by in situ hybridization. Study by high resolution autoradiography using a tritiated oligonucleotide probe].
In situ hybridization (ISH) using a 25 mer tritiated oligonucleotide probe has been performed to study at the electron microscopic level the subcellular localization of the oxytocin mRNA in the rat hypothalamic magnocellular neurons. After high resolution radioautography, silver grains appeared to be localized over the cytoplasm of some magnocellular neurons of the supra-optic nucleus and frequently overlapped the ergastoplasmic "cisternae" of the Nissl bodies. These results demonstrate the possible application of ISH at a subcellular level using high resolution radioautography and a tritiated probe.
Trembleau
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U.A. 339 C.N.R.S., Université de Bordeaux, Talence.
Fevre-Montange
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M
Calas
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D000818:Animals; D001345:Autoradiography; D003593:Cytoplasm / Q000032:analysis; D007031:Hypothalamus / Q000032:analysis / Q000648:ultrastructure*; D008297:Male; D008854:Microscopy, Electron; D009474:Neurons / Q000032:analysis / Q000648:ultrastructure; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D010121:Oxytocin / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D013495:Supraoptic Nucleus / Q000032:analysis / Q000648:ultrastructure; D014316:Tritium
D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D014316:Tritium; D010121:Oxytocin
Localisation ultrastructurale d'ARNm codant pour l'ocytocine par hybridation in situ. Etude par radioautographie à haute résolution à l'aide d'une sonde oligonucléotidique tritiée.