Natural resistance against lymphoma grafts conveyed by H-2Dd transgene to C57BL mice.
The H-2Dd transgenic strain D8 on C57BL background was more resistant to subcutaneous challenge of RBL-5 lymphoma cells than B6 controls. The direct role of the H-2Dd antigen was investigated by the use of (D8 x B6)F1 crosses and (D8 B6) x B6 backcrosses. The latter showed cosegregation with regard to Dd antigen expression and lymphoma resistance, both of which were inherited in a pattern consistent with control by a single dominant gene. The rejection potential in (D8 x B6)F1 mice appeared as strong as that seen in crosses between B6 and MHC congenic mice (on B10 background) carrying H-2Dd. The lymphoma resistance could be abrogated by treatment with anti-asialo GM1 antiserum or anti-NK 1.1 mAb, indicating a role for NK cells.
Höglund
P
P
Department of Tumor Biology, Karolinska Institute, Stockholm, Sweden.
Ljunggren
H G
HG
Ohlén
C
C
Ahrlund-Richter
L
L
Scangos
G
G
Bieberich
C
C
Jay
G
G
Klein
G
G
Kärre
K
K
D000818:Animals; D005786:Gene Expression Regulation; D006183:H-2 Antigens / Q000235:genetics*; D064532:Histocompatibility Antigen H-2D; D007694:Killer Cells, Natural / Q000276:immunology; D008223:Lymphoma / Q000276:immunology*; D008285:Major Histocompatibility Complex; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009368:Neoplasm Transplantation
D006183:H-2 Antigens; D064532:Histocompatibility Antigen H-2D