title: Interactions between cations in modifying the binding of hexokinases I and II to mitochondria.
abstract: Interactions between cations in modifying the binding of hexokinases I and II to mitochondria was examined with reference to the intracellular condition. Mitochondria-binding of either of hexokinases I and II, both prepared from mouse ascites ELD cells, was markedly increased by Mg2+ as has been known well. However, even in the absence of Mg2+, marked binding was attained by 100 mM K+ alone especially for hexokinase I, which seemed generally more ready to bind to mitochondria. On the other hand, the effect of Mg2+ to increase the binding was reduced by the addition of K+, and the decreasing effect of K+ was much more marked for hexokinase II than I. These results indicate that, in addition to Mg2+, monovalent cations as represented by K+, also have marked effect on the binding, and the effect is different for each hexokinases I and II, which may be responsible for the difference in the intracellular distribution between these hexokinases.
authors.affiliation: Department of Physiological Chemistry, Hiroshima University School of Medicine, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Imai
authors.forename: H
authors.initials: H
authors.lastname: Akimoto
authors.forename: M
authors.initials: M
authors.lastname: Oda
authors.forename: H
authors.initials: H
authors.lastname: Okazaki
authors.forename: S
authors.initials: S
authors.lastname: Ishibashi
authors.forename: M
authors.initials: M
authors.lastname: Kurokawa
mesh_terms: D000818:Animals; D001667:Binding, Competitive; D002412:Cations / Q000378:metabolism*; D006593:Hexokinase / Q000378:metabolism*; D007527:Isoenzymes / Q000378:metabolism*; D008274:Magnesium / Q000378:metabolism; D051379:Mice; D008928:Mitochondria / Q000378:metabolism*; D008930:Mitochondria, Liver / Q000378:metabolism*; D011188:Potassium / Q000378:metabolism; D051381:Rats
chemical_list: D002412:Cations; D007527:Isoenzymes; D006593:Hexokinase; D008274:Magnesium; D011188:Potassium