Oligoclonal bands staining for IgG, IgA, IgM, and kappa and lambda light chains in cerebrospinal fluid of patients with multiple sclerosis.
We investigated the oligoclonal bands (OB) in CSF of patients with multiple sclerosis (MS), Guillain-Barré syndrome (GBS) and aseptic meningitis (AS) using polyacryamide or agarose isoelectric focusing following electroblotting and immunostaining for IgG, IgA, IgM and light chains. OB staining for IgG was detected in 80% of patients with MS, 25% with GBS and 13% with AS. OB staining for IgA and IgM was not detected in any disease. In MS, OB were stained only for the kappa or lambda light chains, which was compatible with OB staining for IgG. These results suggest that in MS, OB are monoclonal because they can be stained for gamma heavy chains and for only one light chains.
Araga
S
S
Division of Neurology, Institute of Neurological Sciences, Tottori University School of Medicine, Yonago, Japan.
Irie
H
H
Takahashi
K
K
D006801:Humans; D007070:Immunoglobulin A / Q000134:cerebrospinal fluid; D007074:Immunoglobulin G / Q000134:cerebrospinal fluid; D007147:Immunoglobulin Light Chains / Q000134:cerebrospinal fluid*; D007075:Immunoglobulin M / Q000134:cerebrospinal fluid; D007145:Immunoglobulin kappa-Chains / Q000134:cerebrospinal fluid*; D007146:Immunoglobulin lambda-Chains / Q000134:cerebrospinal fluid*; D007136:Immunoglobulins / Q000134:cerebrospinal fluid*; D008582:Meningitis, Aseptic / Q000276:immunology; D009103:Multiple Sclerosis / Q000276:immunology*; D043025:Oligoclonal Bands; D011129:Polyradiculoneuropathy / Q000276:immunology
D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007147:Immunoglobulin Light Chains; D007075:Immunoglobulin M; D007145:Immunoglobulin kappa-Chains; D007146:Immunoglobulin lambda-Chains; D007136:Immunoglobulins; D043025:Oligoclonal Bands