A distinct terminal structure in newly synthesized chondroitin sulphate chains.
A method was developed for the analysis of non-reducing terminal structure of radiolabelled chondroitin sulphate chains with the aid of N-acetylgalactosamine 4-sulphatase ('terminal 4-sulphatase'), N-acetylgalactosamine 6-sulphatase ('terminal 6-sulphatase'), beta-glucuronidase and beta-N-acetylhexosaminidase. Studies with this method on the non-reducing terminal structure of [35S]sulphate- and [3H]glucose-labelled chondroitin sulphate chains from rat and chick-embryo cartilages showed that the presence of a high proportion of 4-sulphated hexosamine residues is a common feature of the termini of newly synthesized chondroitin sulphate chains. Of the non-reducing terminal 4-sulphated hexosamine residues, about 14% (chick embryo) or 46% (rat) contained an additional sulphate group at position 6. The internal portion of the chondroitin sulphate chains, in contrast, contained little or no 4,6-bis-sulphated hexosamine residue, suggesting that 4,6-bis-sulphated structure may play a role in biosynthetic control at the level of chain termination.
Otsu
K
K
Inoue
H
H
Tsuzuki
Y
Y
Yonekura
H
H
Nakanishi
Y
Y
Suzuki
S
S
D000818:Animals; D001192:Arylsulfatases / Q000302:isolation & purification; D002356:Cartilage / Q000378:metabolism*; D002642:Chick Embryo; D002807:Chondroitin / Q000031:analogs & derivatives*; D002809:Chondroitin Sulfates / Q000096:biosynthesis*; D002852:Chromatography, Ion Exchange; D005947:Glucose / Q000378:metabolism; D005966:Glucuronidase / Q000302:isolation & purification; D006132:Growth Plate / Q000378:metabolism; D006595:Hexosamines / Q000032:analysis; D006596:Hexosaminidases / Q000302:isolation & purification; D066298:In Vitro Techniques; D008722:Methods; D051381:Rats; D001619:beta-N-Acetylhexosaminidases
D006595:Hexosamines; D002807:Chondroitin; D002809:Chondroitin Sulfates; D001192:Arylsulfatases; D006596:Hexosaminidases; D005966:Glucuronidase; D001619:beta-N-Acetylhexosaminidases; D005947:Glucose