Immunoradiometric assay for the rapid detection of HLA-B27.
A new method for the rapid detection of the HLA-B27 antigen is discussed which consists of a direct immunoradiometric assay (IRA) utilizing a 125I-labelled, HLA-B27 specific monoclonal antibody to detect HLA-B27 in whole citrated blood. Thus far, the assay has been used to assign HLA-B27 status in a population study involving 142 individuals; 36 subjects were HLA-B27+ by conventional microcytotoxicity and all 36 were detected by the new IRA and all 106 B27- individuals were also accurately assigned by IRA. The problem of detection of the cross-reactive HLA antigen HLA-B7 was overcome by blocking the reactive sites on HLA-B7 molecules with the HLA-B7 specific antibody, BB7.1. This new assay enables accurate detection of HLA-B27 within 30 min of venepuncture and should facilitate the assessment of HLA-B27 status in tissue typing laboratories.
Trapani
J A
JA
Department of Pathology, University of Melbourne, Parkville, Vic., Australia.
Vaughan
H A
HA
Tait
B D
BD
McKenzie
I F
IF
D000911:Antibodies, Monoclonal; D005069:Evaluation Studies as Topic; D015796:HLA-B27 Antigen / Q000302:isolation & purification*; D006801:Humans; D015592:Immunoradiometric Assay / Q000379:methods*
D000911:Antibodies, Monoclonal; D015796:HLA-B27 Antigen