Cell surface oligosaccharide modulation during differentiation: IV. Normal and transformed cell growth control.
In previous studies it was shown that cell surface oligosaccharide affinity class distributions and binding capacities were down-regulated as normal cells approach senescence. Using a sensitive, amplified, lectin/specific-ligand competition analysis three other growth regulation states were compared to that of cellular senescence. Non-senescent and senescent low-density and contact-induced growth inhibition was compared with neoplastic cell growth control. Non-senescent human fetal lung fibroblasts (IMR-90) down-regulated their mannosyl and galactosyl specificities in response to both low-density and contact-induced growth inhibition. Senescent IMR-90 down-regulate their mannosyl residues in response to contact conditions while they up-regulate their galactosyl residues under the same conditions. Growth-transformed transplantable canine glioma cells did not show density-dependent regulation of their cell surface oligosaccharide structures. Modulation of the CG cells with a specific alpha-mannosidase II inhibitor, Ricinus communis a galactosyl specific lectin, and pokeweed mitogen a cellular differentiating agent resulted in an altered growth phenotype and up-regulation of the mannosyl and galactosyl surface oligosaccharides. These data indicate a controller function for the cell surface oligosaccharides and a general influence on growth control.
Mann
P L
PL
Department of Anatomy, University of New Mexico, School of Medicine, Albuquerque 87131.
Swartz
C M
CM
Holmes
D T
DT
D000470:Alkaloids / Q000494:pharmacology; D001667:Binding, Competitive; D002454:Cell Differentiation; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002462:Cell Membrane / Q000648:ultrastructure*; D002471:Cell Transformation, Neoplastic; D006801:Humans; D037102:Lectins; D009844:Oligosaccharides / Q000032:analysis*; D017026:Swainsonine
D000470:Alkaloids; D037102:Lectins; D009844:Oligosaccharides; D017026:Swainsonine