Developmental regulation of beta-globin gene switching.
A chicken erythroid cell-specific enhancer is located in the intergenic region between the adult beta- and embryonic epsilon-globin genes. In this paper we show that the beta-globin enhancer stimulates transcription of both genes. epsilon-Globin is, however, inappropriately regulated since it is expressed in both embryonic and adult red blood cells. Appropriate stage-specific regulation is observed for both genes when they are present on one plasmid. By analysis of deletion and substitution mutants, we conclude that beta-globin tissue- and developmental stage-specific regulation is mediated by interaction of the beta-globin enhancer with a positive regulatory element within the adult beta-globin promoter, the developmental stage selector element (SSE).
Choi
O R
OR
Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, Illinois 60201.
Engel
J D
JD
D000818:Animals; D002645:Chickens; D002872:Chromosome Deletion; D002874:Chromosome Mapping; D004742:Enhancer Elements, Genetic; D004900:Erythroblasts / Q000378:metabolism; D005914:Globins / Q000235:genetics*; D009154:Mutation; D014158:Transcription, Genetic
D005914:Globins