Determination of adenosine deaminase and purine nucleoside phosphorylase activities using high-performance liquid chromatographic and radiochromatographic methods.
Two methods for the determination of adenosine deaminase and purine nucleoside phosphorylase activities were compared. The high-performance liquid chromatographic (HPLC) technique used separation on a reversed-phase silica column and exhibited adequate sensitivity and a markedly higher rate of analysis compared with that of the paper radiochromatographic method. Correlation analysis of the results obtained by the two methods on a set of lymphoid cells from 25 patients with lympho-proliferative disorders confirmed the utility of the HPLC technique in clinical investigations.
Bohácek
J
J
Institute of Biophysics, Czechoslovak Academy of Sciences, Brno.
Hosek
B
B
Babusíková
O
O
Hrivnáková
A
A
D000243:Adenosine Deaminase / Q000032:analysis* / Q000097:blood; D001345:Autoradiography; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D008214:Lymphocytes / Q000201:enzymology*; D009700:Nucleoside Deaminases / Q000032:analysis*; D010430:Pentosyltransferases / Q000032:analysis*; D011683:Purine-Nucleoside Phosphorylase / Q000032:analysis* / Q000097:blood
D010430:Pentosyltransferases; D011683:Purine-Nucleoside Phosphorylase; D009700:Nucleoside Deaminases; D000243:Adenosine Deaminase