[Extraction of chromosomal DNA from Staphylococcus and Listeria by a rapid method using achromopeptidase].
A rapid and simple method for preparation of chromosomal DNA from Gram-positive bacteria is reported. Susceptibility to lysis with Sodium Dodecyl Sulfate (SDS) increases when undergoing treatment with acetone before being digested by bacteriolytic enzymes. Rapid lysis of Staphylococcus and Listeria cells is obtained through a respective treatment by lysozyme with lysostaphine and by lysozyme with achromopeptidase, adding to that the effect of SDS in Tris-Hcl buffer. This procedure of preparing chromosomal DNA provides 1 to 4 mg of DNA out of 1 g of bacterial cells in a day.
Boujaafar
N
N
Laboratoire de Microbiologie, C.H.U.F. Hached, Sousse, Tunisie.
Jeddi
M
M
Freney
J
J
D002875:Chromosomes / Q000032:analysis*; D004269:DNA, Bacterial / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D008089:Listeria monocytogenes / Q000032:analysis*; D012697:Serine Endopeptidases; D013210:Staphylococcus / Q000032:analysis*; D013211:Staphylococcus aureus / Q000032:analysis; D013212:Staphylococcus epidermidis / Q000032:analysis
D004269:DNA, Bacterial; D012697:Serine Endopeptidases; C036813:lysyl endopeptidase
Extraction de l'ADN chromosomique de Staphylococcus et de Listeria par une méthode rapide à l'achromopeptidase.