In vivo effects of Sn-1,2-dioctanoylglycerol, TPA and A23187 on hamster cheek pouch epithelium.
Cheek pouches of male Syrian golden hamsters were topically treated with a single dose of TPA (.5 microgram), calcium ionophore A23187 (75 micrograms) or Sn-1,2-dioctanoylglycerol (DiC8) (500 micrograms) dissolved in 0.25 ml acetone. Acetone-treated animals served as controls. After 48 h the mitotic index for the control group was 1.1 +/- 0.1 per 1 mm of the basement membrane length. All the test congeners exhibited higher mitotic indices than controls: TPA (4.8 +/- 0.4), A23187 (3.9 +/- 0.3), DiC8 (2.1 +/- 0.2). All groups exhibited an increase in the epithelial thickness manifested by cellular hyperplasia. The treatment of the pouches with the anti-inflammatory agent fluocinolone acetonide inhibited the mitogenic and hyperplasiogenic affects on the epithelium induced by the various test chemicals. These studies indicate a possible role of calcium-phospholipid dependent protein kinase (protein kinase C) in the mediation of oral epithelial cell proliferation.
Singh
B B
BB
Department of Oral Pathology, Medical College of Georgia, Augusta 30912.
McKinney
R V
RV
Schuster
G S
GS
Erbland
J F
JF
Baker
R L
RL
D000818:Animals; D000001:Calcimycin / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002610:Cheek; D003238:Connective Tissue / Q000187:drug effects; D003239:Connective Tissue Cells; D006224:Cricetinae; D004075:Diglycerides / Q000494:pharmacology*; D004847:Epithelial Cells; D004848:Epithelium / Q000187:drug effects; D005446:Fluocinolone Acetonide / Q000494:pharmacology; D005989:Glycerides / Q000494:pharmacology*; D006965:Hyperplasia; D008297:Male; D008647:Mesocricetus; D008938:Mitosis / Q000187:drug effects; D009061:Mouth Mucosa / Q000166:cytology / Q000187:drug effects*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D004075:Diglycerides; D005989:Glycerides; D005446:Fluocinolone Acetonide; C044295:1,2-dioctanoylglycerol; D000001:Calcimycin; D013755:Tetradecanoylphorbol Acetate