The effect of chronic low level lead exposure on blood-brain barrier function in the developing rat.
Blood-brain barrier (BBB) function was assessed in 19-21-day-old rats exposed to low level lead from birth. Newborn rats received lead via milk from lactating dams given drinking water containing 0.1% lead acetate [Pb(Ac)2]. The treatment regime produced lead levels in the neonates within the range 20-80 micrograms dl-1 blood, without affecting growth. Cerebrovascular permeability (PS-product) to the diffusion-limited solute mannitol was unchanged in six regions of the cerebral hemisphere, the cerebellum and the brainstem, suggesting that barrier integrity was not affected by the low dose lead treatment. Regional cerebrovascular permeability to nutrient tracers representing seven BBB transport classes was not impaired by lead treatment. However, the PS estimates for the amino acids lysine and histidine and for thiamine were greater than control in some regions of the cerebral hemisphere. These alterations in nutrient supply to the brain may reflect altered substrate utilization associated with repair processes or delayed maturation of the CNS.
Moorhouse
S R
SR
Department of Pharmacology, British Industrial Biological Research Association, Carshalton, Surrey, U.K.
Carden
S
S
Drewitt
P N
PN
Eley
B P
BP
Hargreaves
R J
RJ
Pelling
D
D
D000818:Animals; D000831:Animals, Newborn; D001812:Blood-Brain Barrier; D001921:Brain / Q000187:drug effects / Q000254:growth & development*; D002199:Capillary Permeability / Q000187:drug effects; D002560:Cerebrovascular Circulation / Q000187:drug effects; D004058:Diffusion; D006639:Histidine / Q000493:pharmacokinetics; D007855:Lead Poisoning / Q000503:physiopathology*; D008239:Lysine / Q000493:pharmacokinetics; D008353:Mannitol / Q000493:pharmacokinetics; D009942:Organometallic Compounds / Q000008:administration & dosage; D051381:Rats; D011919:Rats, Inbred Strains; D013831:Thiamine / Q000493:pharmacokinetics
D009942:Organometallic Compounds; D008353:Mannitol; D006639:Histidine; D008239:Lysine; C008261:lead acetate; D013831:Thiamine