Development of an enzyme-linked immunosorbent assay for detection of IgE antibodies specific to Dermatophagoides pteronyssinus.
Allergy to Dermatophagoides pteronyssinus was determined in 61 rhinitis patients using prick test (PT), enzyme-immunoassay (EIA) and enzyme-linked immunosorbent assay (ELISA). A total of 43 patients tested positive with PT. Forty six patients were positive when tested with EIA and ELISA. With PT as standard test, EIA was found to have 83.7% sensitivity and 44.4% specificity; ELISA had 81.4% sensitivity and 38.9% specificity. There was a linear relationship between absorbance values obtained by EIA and ELISA. The performance time was 8 hours, 24 hours and 30 minutes for ELISA, EIA and PT respectively. The cost per test for ELISA, EIA and PT was US$ 0.20, US$ 5.20 and US$ 0.14 respectively. It was concluded that ELISA was more cost-effective than EIA should be used to supplement PT for a more complete diagnosis of allergy.
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Division of Acarology, Institute for Medical Research, Kuala Lumpur, Malaysia.
Yit
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Husain
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D000818:Animals; D003362:Cost-Benefit Analysis; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D006967:Hypersensitivity / Q000175:diagnosis; D007124:Immunoenzyme Techniques; D007073:Immunoglobulin E / Q000032:analysis*; D008296:Malaysia; D008925:Mites / Q000276:immunology*; D012680:Sensitivity and Specificity; D012882:Skin Tests / Q000379:methods
D007073:Immunoglobulin E