title: High fidelity of guanine translation in a plasmid-directed in vitro system.
abstract: The extent of misreading of individual bases in the first or second codon position has been measured in vitro in a simplified plasmid-directed coupled system in which natural messenger translation is restricted to the formation of the N-terminal di- or tripeptide. Experiments were performed under conditions of competition between cognate and noncognate tRNAs in the presence of streptomycin to maximize the frequency of reading errors. A striking lack of susceptibility to mistranslation of guanine, as compared to the other 3 bases, was observed.
authors.affiliation: Laboratoire de Biologie Moléculaire, Université de Lyon, Villeurbanne, France.
authors.forename: D
authors.initials: D
authors.lastname: Negre
authors.forename: Y
authors.initials: Y
authors.lastname: Cenatiempo
authors.forename: A J
authors.initials: AJ
authors.lastname: Cozzone
mesh_terms: D002474:Cell-Free System; D003062:Codon; D004926:Escherichia coli; D005786:Gene Expression Regulation; D006147:Guanine / Q000378:metabolism*; D010957:Plasmids; D014176:Protein Biosynthesis; D012333:RNA, Messenger; D012346:RNA, Transfer, Amino Acyl / Q000378:metabolism; D013307:Streptomycin / Q000494:pharmacology
chemical_list: D003062:Codon; D012333:RNA, Messenger; D012346:RNA, Transfer, Amino Acyl; D006147:Guanine; D013307:Streptomycin