title: Suppressed development of cultured mouse and swine embryos by diethylstilbestrol.
abstract: Effects of prolonged exposure to the synthetic estrogen diethylstilbestrol (DES) on in vitro development of early mouse and swine embryos were investigated. Two-cell mouse embryos cultured in Whitten's medium (WM) for 192 h were exposed to 10(-4), 10(-7) or 10(-10) M DES dissolved in 1, 10(-3) or 10(-6)% ethanol, respectively. One-cell to eight-cell swine embryos were cultured in WM for 192 h containing 10(-4) or 10(-7) M DES dissolved in 1 and 10(-3)% ethanol, respectively. Embryos cultured in WM containing 1 (0 DES1), 10(-3) (0 DES2) or 10(-6)% ethanol (0 DES3) served as controls. Hatching was inhibited (P less than .05) in mouse embryos cultured in 10(-4) M DES (3.0 +/- 2.1% vs 0 DES1, 25.1 +/- 3.7%). Similar (P greater than .10) percentages of mouse embryos hatched in 10(-7) M DES (36.4 +/- 5.4% vs 0 DES2, 29.1 +/- 5.7%) and 10(-10) M DES (44.4 +/- 4.4% vs 0 DES3, 38.9 +/- 5.3%). Diethylstilbestrol at a concentration of 10(-4) M failed to affect the development of one- to eight-cell swine embryos into blastocysts. However, compared with 0 DES2, 10(-7) M DES reduced (P less than .05) the number of swine blastocysts developing from one- to two-cell (36 vs 78%) and three- to four-cell embryos (50 vs 84%). No significant effects of 10(-7) M DES were detected on the ability of six- to eight-cell swine embryos to develop into blastocysts.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Dept. of Anim. Sci., Oregon State University, Corvallis 97331-6702.
authors.forename: C S
authors.initials: CS
authors.lastname: Gardiner
authors.forename: A R
authors.initials: AR
authors.lastname: Menino
authors.forename: A E
authors.initials: AE
authors.lastname: Archibong
authors.forename: J S
authors.initials: JS
authors.lastname: Williams
authors.forename: F
authors.initials: F
authors.lastname: Stormshak
authors.forename: D C
authors.initials: DC
authors.lastname: England
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000187:drug effects*; D002970:Cleavage Stage, Ovum / Q000187:drug effects*; D046508:Culture Techniques; D004054:Diethylstilbestrol / Q000494:pharmacology*; D005260:Female; D051379:Mice / Q000196:embryology*; D009028:Morula / Q000187:drug effects*; D013552:Swine / Q000196:embryology*
chemical_list: D004054:Diethylstilbestrol