title: Plasminogen activator-anti-human fibrinogen conjugate.
abstract: A covalent conjugate between the plasminogen activator urokinase and polyclonal rabbit anti-human fibrinogen has been formed using the heterobifunctional coupling reagent N-succinimidyl 3-(2-pyridyldithio) propionate. The resultant urokinase-anti-human fibrinogen conjugate was separated from unreacted material by gel filtration. The conjugate exhibited amidase activity against the small chromogenic substrate pyroglutamyl-glycyl-arginine-p-nitroanilide as well as plasminogen activator activity in an assay employing plasminogen and the plasmin substrate D-valyl-leucyl-lysine-p-nitroanilide. Retention of antibody specificity for fibrinogen was demonstrated using an enzyme linked immunoassay procedure. The conjugate was found to have greater stability in human plasma than unconjugated urokinase.
authors.forename: C L
authors.initials: CL
authors.lastname: Sevilla
authors.forename: N H
authors.initials: NH
authors.lastname: Mahle
authors.forename: C M
authors.initials: CM
authors.lastname: Boylan
authors.forename: D M
authors.initials: DM
authors.lastname: Callewaert
mesh_terms: D000906:Antibodies; D003432:Cross-Linking Reagents; D005340:Fibrinogen / Q000276:immunology*; D005341:Fibrinolysin / Q000378:metabolism; D006801:Humans; D013379:Substrate Specificity; D014568:Urokinase-Type Plasminogen Activator
chemical_list: D000906:Antibodies; D003432:Cross-Linking Reagents; D005340:Fibrinogen; D005341:Fibrinolysin; D014568:Urokinase-Type Plasminogen Activator