title: VIP potentiates cholinergic effects on the mucociliary system in the maxillary sinus.
abstract: The neuropeptide vasoactive intestinal polypeptide (VIP), which is found in a population of cholinergic parasympathetic neurons in the airways, has no effects per se on mucociliary activity. In order to test the hypothesis that VIP may modulate cholinergic regulation of the mucociliary system, VIP was infused intraarterially (8.4 pmol/kg/min), and the response to challenges with methacholine in the maxillary sinus of rabbits were recorded with a photoelectric technique. Occurrence of VIP-like immunoreactivity in the rabbit maxillary sinus, maxillary nerve, and sphenopalatine ganglion was investigated. Immunoreactivity against VIP was found in nerve fibers in the subepithelial layer of the maxillary sinus and in numerous nerve cell bodies in the sphenopalatine ganglion. Infusion of VIP potentiated the mucociliary increase induced by methacholine. The mucociliary wave frequency change increased from 6.1% +/- 1.7% to 13.3% +/- 3.9% (0.01 micrograms/kg methacholine), from 11.6% +/- 3.6% to 18.8% +/- 2.2% (0.05 micrograms/kg) and from 17.0% +/- 3.0% to 27.4% +/- 3.6% (0.1 micrograms/kg). Both peak responses and response durations increased during infusions. In contrast, the vasodilating agent papaverine sulphate did not influence the mucociliary response to methacholine. The modulating effect of VIP on the mucociliary system, taken together with the morphologic observations, suggest that VIP may have a physiologic role in the regulation of the mucociliary system in the maxillary sinus.
authors.affiliation: Department of Oto-Rhino-Laryngology, University Hospital of Lund, Sweden.
authors.forename: S
authors.initials: S
authors.lastname: Lindberg
authors.forename: A
authors.initials: A
authors.lastname: Cervin
authors.forename: U
authors.initials: U
authors.lastname: Mercke
authors.forename: R
authors.initials: R
authors.lastname: Uddman
mesh_terms: D000818:Animals; D002923:Cilia / Q000502:physiology; D007150:Immunohistochemistry; D008443:Maxillary Sinus / Q000378:metabolism / Q000502:physiology*; D016210:Methacholine Chloride; D008688:Methacholine Compounds / Q000494:pharmacology; D009079:Mucociliary Clearance / Q000187:drug effects*; D009092:Mucous Membrane / Q000378:metabolism / Q000502:physiology; D010208:Papaverine / Q000494:pharmacology; D011817:Rabbits; D014660:Vasoactive Intestinal Peptide / Q000378:metabolism / Q000494:pharmacology / Q000502:physiology*
chemical_list: D008688:Methacholine Compounds; D016210:Methacholine Chloride; D014660:Vasoactive Intestinal Peptide; D010208:Papaverine