title: Reinitiation during lambda DNA replication resulting from either cis-Pt treatment or infection of a P2 lysogenic strain.
abstract: Nested areas of replication are observed in phage lambda replicative intermediates and arise from reinitiation from the lambda origin. Reinitiation occurs when the first round of lambda replication takes place in the presence of the drug cis-Pt or when lambda infects a host which has been preincubated with the drug. In the latter case it is shown that the infection proceeds during the expression of SOS functions induced in the host as a result of the drug treatment. When lambda infects a host lysogenic for phage P2, an interference process occurs which prevents formation of lambda phage. The lambda DNA does, however, undergo at least one round of replication but is abnormal in that lambda origins reinitiate to form nested areas of replication similar to those resulting from exposure to the drug cis-Pt.
authors.forename: M
authors.initials: M
authors.lastname: Schnös
authors.forename: R B
authors.initials: RB
authors.lastname: Inman
mesh_terms: D010582:Bacteriophage lambda / Q000187:drug effects / Q000235:genetics* / Q000648:ultrastructure; D002945:Cisplatin / Q000494:pharmacology; D004261:DNA Replication / Q000187:drug effects; D004926:Escherichia coli / Q000235:genetics* / Q000648:ultrastructure; D008242:Lysogeny; D008854:Microscopy, Electron; D011938:Rec A Recombinases / Q000032:analysis
chemical_list: D011938:Rec A Recombinases; D002945:Cisplatin