title: Sequential changes in red cell glycolytic enzymes and intermediates and possible control mechanisms in the first two months of postnatal life in lambs.
abstract: The sequential changes in several glycolytic enzymes, glucose-6-phosphate dehydrogenase, glycolytic intermediates, and adenosine triphosphate, as well as intracellular pH and plasma inorganic phosphorus were followed simultaneously in eight lambs from birth to 2 months of age. The activities of all glycolytic enzymes and glucose-6-phosphate dehydrogenase were elevated at birth. The 2,3-diphosphoglycerate concentration increased markedly postnatally and was associated with a simultaneous increase in the concentrations of red cell glucose-6-phosphate and total triose phosphate and a decrease in intracellular pH. Inorganic phosphorus also increased and correlated with the 2,3-diphosphoglycerate concentration in the first 10 days of postnatal life. The content of red cell 3-phosphoglycerate, 2-phosphoglycerate, phosphoenolpyruvate, and ATP increased slightly. These results suggested increased glycolytic flux through the diphosphoglycerate mutase reaction which resulted in net synthesis of 2,3-diphosphoglycerate. The red cell total triose phosphate peaked and fell initially, followed by glucose-6-phosphate and 2,3-diphosphoglycerate suggesting inhibition of phosphofructokinase activity and a decrease in glycolysis secondary to decreased red cell intracellular pH. After 10 days of postnatal life all glycolytic intermediates fell simultaneously, which correlated with a decrease in activity of the glycolytic enzymes.
authors.forename: S F
authors.initials: SF
authors.lastname: Travis
authors.forename: L C
authors.initials: LC
authors.lastname: Wagerle
authors.forename: C M
authors.initials: CM
authors.lastname: De Alvarado
authors.forename: G
authors.initials: G
authors.lastname: Rose
authors.forename: M
authors.initials: M
authors.lastname: Delivoria-Papadopoulos
mesh_terms: D000375:Aging; D000818:Animals; D000831:Animals, Newborn / Q000097:blood* / Q000378:metabolism; D001731:Bisphosphoglycerate Mutase / Q000097:blood; D004912:Erythrocytes / Q000201:enzymology*; D005634:Fructose-Bisphosphate Aldolase / Q000097:blood; D005954:Glucosephosphate Dehydrogenase / Q000097:blood; D006019:Glycolysis; D006593:Hexokinase / Q000097:blood; D010732:Phosphofructokinase-1 / Q000097:blood; D010735:Phosphoglycerate Kinase / Q000097:blood; D010751:Phosphopyruvate Hydratase / Q000097:blood; D010758:Phosphorus / Q000097:blood; D011770:Pyruvate Kinase / Q000097:blood; D012756:Sheep; D013403:Sugar Phosphates / Q000097:blood
chemical_list: D013403:Sugar Phosphates; D010758:Phosphorus; D005954:Glucosephosphate Dehydrogenase; D006593:Hexokinase; D010732:Phosphofructokinase-1; D011770:Pyruvate Kinase; D010735:Phosphoglycerate Kinase; D005634:Fructose-Bisphosphate Aldolase; D010751:Phosphopyruvate Hydratase; D001731:Bisphosphoglycerate Mutase