Phosphatidylcholine and phosphatidylethanolamine derivatives, membrane fluidity and changes in the lipolytic activity of ram spermatozoa plasma membranes during cryoconservation.
1. A decrease of the alkenyl-acyl derivatives and an increase of the diacyl derivatives of PC and PE were observed after cryoconservation. 2. A diminution of membrane-bound phospholipase A2 activity was observed after cryoconservation. The activity of neutral sphingomyelinase remained unchanged. 3. The enrichment of plasma membranes with DPPC as well as the addition of the cryoprotector of Nagase-Niwa were observed to protect the membranes from fluidization.
Hinkovska-Galcheva
V
V
Central Laboratory of Biophysics, Bulgarian Academy of Sciences, Sofia.
Peeva
D
D
Momchilova-Pankova
A
A
Petkova
D
D
Koumanov
K
K
D015060:1,2-Dipalmitoylphosphatidylcholine / Q000494:pharmacology; D000818:Animals; D002462:Cell Membrane / Q000201:enzymology; D003451:Cryoprotective Agents / Q000494:pharmacology; D005615:Freezing; D008066:Lipolysis; D008297:Male; D008560:Membrane Fluidity; D008565:Membrane Proteins / Q000378:metabolism; D010713:Phosphatidylcholines / Q000378:metabolism*; D010714:Phosphatidylethanolamines / Q000378:metabolism*; D010741:Phospholipases A / Q000378:metabolism; D054467:Phospholipases A2; D011309:Preservation, Biological; D012756:Sheep; D013094:Spermatozoa / Q000201:enzymology*; D013108:Sphingomyelin Phosphodiesterase / Q000378:metabolism
D003451:Cryoprotective Agents; D008565:Membrane Proteins; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D015060:1,2-Dipalmitoylphosphatidylcholine; D010741:Phospholipases A; D054467:Phospholipases A2; D013108:Sphingomyelin Phosphodiesterase