Release of hemopoietic factors by normal human T cell lines with either suppressor or helper activity.
We analyzed the release of activities capable of stimulating the in vitro growth of human hemopoietic progenitor cells by long-term cultured T cell growth factor (TCGF)-dependent human T lymphocytes. Seven cell lines tested produced colony-stimulating activity (CSA) as well as burst-promoting activity (BPA). The CSA stimulated primarily the growth of the cells forming colonies after 14 days of incubation. In addition the supernatants from these seven T-cell lines showed the ability to induce the in vitro growth of mixed granulocyte, erythroid, megakaryocyte, macrophage colonies (CFU-GEMM). The release of hemopoietic factors did not depend on the presence of accessory cells or phytohemagglutinin or serum during the incubation for factor production. In six of the T cell lines the majority of the cells were reactive to the OKT 8 monoclonal antibody (MoAb), whereas one cell line contained mostly OKT 4+ cells. Suppressor activity was detected in three tested OKT 8+ cell lines, while the one OKT 4+ displayed helper activity. All cell lines produced hemopoietic factors with equal efficiency. These results indicate that factors affecting human hematopoiesis are produced by normal T lymphocytes in long-term culture and this property is not related to the helper or suppressor activity of the cultured cells.
Lanfrancone
L
L
Ferrero
D
D
Gallo
E
E
Foa
R
R
Tarella
C
C
D000328:Adult; D001853:Bone Marrow / Q000276:immunology; D002460:Cell Line; D003115:Colony-Stimulating Factors / Q000378:metabolism*; D046508:Culture Techniques / Q000379:methods; D006801:Humans; D007376:Interleukin-2 / Q000502:physiology; D010641:Phenotype; D013601:T-Lymphocytes / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*
D003115:Colony-Stimulating Factors; D007376:Interleukin-2