Inhibition of adriamycin-promoted microsomal lipid peroxidation by beta-carotene, alpha-tocopherol and retinol at high and low oxygen partial pressures.
Iron-dependent peroxidation of rat liver microsomes, enhanced by adriamycin, was measured in the presence of increasing concentrations of alpha-tocopherol, beta-carotene and retinol at low and high pO2. beta-Carotene and alpha-tocopherol inhibited lipid peroxidation by more than 60% when present at concentrations greater than 50 nmol/mg microsomal protein at both high and low pO2. Retinol inhibited peroxidation by 39% at concentrations greater than 100 nmol/mg microsomal protein. This maximal level of inhibition by retinol was unaltered by pO2. However, beta-carotene was more effective than alpha-tocopherol or retinol at a pO2 of 4 mmHg, whereas alpha-tocopherol was more effective under aerobic conditions. Since adriamycin-dependent lipid peroxidation is maximal at low pO2, beta-carotene may play a role in protecting against this process.
Vile
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GF
Department of Pathology, Christchurch School of Medicine, Christchurch Hospital, New Zealand.
Winterbourn
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CC
D000818:Animals; D002338:Carotenoids / Q000494:pharmacology*; D004317:Doxorubicin / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects*; D008862:Microsomes, Liver / Q000378:metabolism*; D009249:NADP / Q000494:pharmacology; D010100:Oxygen / Q000494:pharmacology; D010313:Partial Pressure; D051381:Rats; D011919:Rats, Inbred Strains; D014801:Vitamin A / Q000494:pharmacology*; D014810:Vitamin E / Q000494:pharmacology*; D019207:beta Carotene
D019207:beta Carotene; D014801:Vitamin A; D014810:Vitamin E; D002338:Carotenoids; D009249:NADP; D004317:Doxorubicin; D010100:Oxygen