title: Long-term cultivation of T-cell subsets from patients with acquired immune deficiency syndrome.
abstract: Peripheral blood mononuclear cells from patients with acquired immune deficiency syndrome (AIDS) and from healthy controls have been cultured in vitro in the presence of phytohemagglutinin (PHA) and interleukin 2 (IL-2). The T-cell subsets that grew were of both helper and suppressor type within the first week, but after 1-3 months, T cells with a suppressor/cytotoxic phenotype predominated. The lymphocytes from AIDS patients responded less effectively to the culture conditions employed. These results indicate that IL-2 can be used to maintain both major subsets of T cells from AIDS patients as well as healthy controls for short periods. However, in both situations, the helper phenotype is selectively reduced after one month in culture.
authors.forename: J A
authors.initials: JA
authors.lastname: Levy
authors.forename: L H
authors.initials: LH
authors.lastname: Tobler
authors.forename: T M
authors.initials: TM
authors.lastname: McHugh
authors.forename: C H
authors.initials: CH
authors.lastname: Casavant
authors.forename: D P
authors.initials: DP
authors.lastname: Stites
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology* / Q000473:pathology; D002455:Cell Division; D002478:Cells, Cultured; D006801:Humans; D007376:Interleukin-2 / Q000276:immunology; D008297:Male; D010835:Phytohemagglutinins / Q000494:pharmacology; D013601:T-Lymphocytes / Q000145:classification / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology
chemical_list: D007376:Interleukin-2; D010835:Phytohemagglutinins