Detection of Listeria spp. in faeces from animals, in feeds, and in raw foods of animal origin.
Thirty-nine samples of feeds and 79 faecal samples were collected at seven different dairy farms, at some of which the cows were suffering from Listeria mastitis. Faecal samples were also collected from poultry on a dairy farm and from cages used for transportation of poultry to slaughter. Also neck-skin samples were taken from 17 carcasses of dressed poultry, and five samples of scalding and chilling water at a poultry slaughterhouse. Finally, 67 samples of minced beef were collected from retail shops. The overall results show that approximately 82% of the feed samples harboured Listeria spp. and 62% Listeria monocytogenes. The faecal samples showed that 67% harboured Listeria spp. and 51% L. monocytogenes. In the minced beef samples, Listeria spp. could be demonstrated in 67% and L. monocytogenes in 28%. Of the faecal samples from poultry, 33% harboured Listeria spp. and also 33% L. monocytogenes. Listeria spp. were detected in 94% of the poultry neck-skin samples, and L. monocytogenes in 47%. Almost all L. monocytogenes from faeces and feeds agglutinated Listeria antisera against serotypes 1-4, while only 71% of the strains from minced beef agglutinated the same antisera. The high prevalence of positive findings indicates that the isolation method used is suitable for detection of Listeria spp. in heavily contaminated material as well as in foods with low bacterial counts.
Skovgaard
N
N
Institute of Hygiene and Microbiology, Royal Veterinary and Agricultural University, Frederiksberg, Denmark.
Morgen
C A
CA
D000003:Abattoirs; D000821:Animal Feed; D000818:Animals; D002417:Cattle; D002645:Chickens; D015169:Colony Count, Microbial; D005243:Feces / Q000382:microbiology*; D005516:Food Microbiology; D008089:Listeria monocytogenes / Q000302:isolation & purification*; D008460:Meat