[Comparative studies of the determination of T lymphocytes in human peripheral blood].
Different methods for determination of T-lymphocytes in human peripheral blood were compared: rosetting with sheep erythrocytes (SRBC), AET-treated SRBC, immunofluorescence using a monoclonal antibody against T cells (BL-T2), complement dependent cytolysis with polyclonal antisera against thymocytes, cytochemical demonstration of unspecific acid alpha-naphthyl-acetate esterase (ANAE), and electrophoretic mobility using a cell electrophoresis system (PARMOQUANT 2). Depending on the method, mean values between 70 and 79% T cells among separated mononuclear cells (MNC) were found. All paired observations were subjected to statistical analysis using rank correlation and U-test. From this analysis it is concluded that rosetting with SRBC, immunofluorescence using the monoclonal T-cell antibody and cytochemical reactivity for ANAE are favored methods for determining the T cell content of human MNC. However, the monoclonal antibody BL-T2 and the ANAE are not generally applicable because both markers were also found on malignant B-lymphocytes (B-CLL).
Schön
E
E
Götze
D
D
Mansfeld
H W
HW
Trappe
C
C
Lambrecht
R
R
Ansorge
S
S
D000328:Adult; D000911:Antibodies, Monoclonal; D000961:Antilymphocyte Serum; D004586:Electrophoresis; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009283:Naphthol AS D Esterase / Q000378:metabolism; D012397:Rosette Formation; D013223:Statistics as Topic; D013601:T-Lymphocytes / Q000201:enzymology / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000961:Antilymphocyte Serum; D009283:Naphthol AS D Esterase
Vergleichende Untersuchungen zur Bestimmung von T-Lymphozyten im peripheren Blut des Menschen.