Production of prostaglandins and HETE-compounds by isolated-cultivated bovine aortic endothelial cells.
The main product of AA conversion by BAEC is PGI2. Other PGs were synthesized by confluent cells only in traces with exception of a HHT-like compound which has still to be characterized in more details. A significant release of HETE was only detected when BAEC were destroyed. Under these conditions about 1 pmole/10(5) cells 15- and 12 HETE were released by BAEC. Taken into account the amounts of HETE produced by endothelial cells as well as the partially contradictory activities of 12- and 15-HETE we suggest that an acclerated lipoxygenase activity reflects a lesion of endothelium, but is of minor physiological relevance as compared to the generation of HETE and other eicosanoids by blood cells.
Oestreich
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Diabetesforschungsinstitut, Düsseldorf, FRG.
Rösen
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D015117:5,8,11,14-Eicosatetraynoic Acid / Q000494:pharmacology; D000818:Animals; D001011:Aorta; D000001:Calcimycin / Q000494:pharmacology; D002417:Cattle; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism*; D006893:Hydroxyeicosatetraenoic Acids / Q000096:biosynthesis*; D011453:Prostaglandins / Q000096:biosynthesis*
D006893:Hydroxyeicosatetraenoic Acids; D011453:Prostaglandins; D015117:5,8,11,14-Eicosatetraynoic Acid; D000001:Calcimycin