The effects of glucose and insulin on beta-hexosaminidase in cultured fibroblasts from diabetic and non-diabetic subjects.
The effects of glucose and insulin in culture medium on the release and cellular content of beta-hexosaminidase were studied in fibroblast cultures obtained from patients with type II diabetes and from control subjects. The release of beta-hexosaminidase was (positively) correlated to extracellular glucose concentration. The cellular activity was not influenced by glucose. Insulin had no effect on the cellular activity or the release of beta-hexosaminidase. The results were similar for both cell types. It is proposed that increased plasma levels of lysosomal hydrolases (e.g. beta-hexosaminidase) from human diabetics might be due to increased release from cells exposed to elevated glucose concentrations.
Hultberg
B
B
Isaksson
A
A
Kjellström
T
T
D002478:Cells, Cultured; D003924:Diabetes Mellitus, Type 2 / Q000201:enzymology*; D005347:Fibroblasts / Q000201:enzymology*; D005947:Glucose / Q000494:pharmacology*; D006596:Hexosaminidases / Q000378:metabolism*; D006801:Humans; D007328:Insulin / Q000494:pharmacology*; D007525:Isoelectric Focusing; D001619:beta-N-Acetylhexosaminidases
D007328:Insulin; D006596:Hexosaminidases; D001619:beta-N-Acetylhexosaminidases; D005947:Glucose