Ia-like antigens on T-cells and their subpopulations in pulmonary sarcoidosis and in hypersensitivity pneumonitis. Analysis of bronchoalveolar and blood lymphocytes.
We investigated the expression of Ia antigens on T-cells from lung and blood, as a sign of T-cell activation, in 17 patients with active pulmonary sarcoidosis, 12 patients with inactive sarcoidosis, 9 patients with hypersensitivity pneumonitis, and 10 normal control subjects. Lymphocyte subsets were identified by mouse monoclonal antibodies using a peroxidase-antiperoxidase method. Patients with active sarcoidosis and patients with hypersensitivity pneumonitis had a significant increase in Ia+ T-cells in bronchoalveolar lavage fluid compared with that in patients with inactive sarcoidosis and that in control subjects (p less than 0.01). Blood T-cells from the same patients did not show this sign of activation. The highest numbers of Ia+ T-cells were recovered from the lungs of patients with hypersensitivity pneumonitis, indicating the high state of activation of immunoregulatory T-cells in this disease. Additional analysis revealed that in sarcoidosis, Ia+ lung T-cells were exclusively of the OKT4+ helper phenotype, whereas in hypersensitivity pneumonitis, OKT4+ helper as well as OKT8+ suppressor lung cells expressed in part Ia antigens. These observations suggest that different T-cell subpopulations are activated in sarcoidosis and in hypersensitivity pneumonitis.
Costabel
U
U
Bross
K J
KJ
Rühle
K H
KH
Löhr
G W
GW
Matthys
H
H
D000293:Adolescent; D000328:Adult; D000368:Aged; D000542:Alveolitis, Extrinsic Allergic / Q000097:blood / Q000276:immunology*; D001980:Bronchi / Q000166:cytology; D001999:Bronchoscopy; D005260:Female; D000949:Histocompatibility Antigens Class II / Q000032:analysis / Q000276:immunology*; D006801:Humans; D008171:Lung Diseases / Q000097:blood / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D011014:Pneumonia / Q000097:blood / Q000276:immunology; D011650:Pulmonary Alveoli / Q000166:cytology; D012507:Sarcoidosis / Q000097:blood / Q000276:immunology*; D013601:T-Lymphocytes / Q000032:analysis / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology; D007507:Therapeutic Irrigation
D000949:Histocompatibility Antigens Class II