The use of acrylic copolymers as antibody carriers for immunoaffinity chromatography.
Among several various acrylic copolymer used as carriers for anti-bovine serum albumin the best are copolymers obtained from acrylonitrile and ethyl or butyl acrylate crosslinked with divinylbenzene which after aminolysis with di- or triamines were activated with glutardialdehyde. The immunoadsorbents thus obtained were successfully used for purification of bovine serum albumin from bovine serum or from crude fraction of the albumin by immunoaffinity chromatography. Electrophoretically pure bovine albumin was eluted from immunoadsorbent column with deionised water and with glycine/HCl buffer, pH 2.5. The albumin was contamined with less than 0.1% of bovine immunoglobulins and with 0.05% of rabbit immunoglobulins. No contamination with rabbit immunoglobulin was observed when immunoadsorbents were pre-treated with sodium borohydride.
Rapak
A
A
Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw.
Szewczuk
A
A
Kolarz
B N
BN
Trochimczuk
A
A
Wojaczyńska
M
M
D000181:Acrylonitrile; D000201:Activation Analysis / Q000379:methods; D000818:Animals; D000936:Antigen-Antibody Complex; D002846:Chromatography, Affinity / Q000295:instrumentation*; D005976:Glutaral; D007164:Immunosorbents; D009570:Nitriles; D011817:Rabbits; D012710:Serum Albumin, Bovine / Q000276:immunology / Q000302:isolation & purification*; D014753:Vinyl Compounds
D000936:Antigen-Antibody Complex; D007164:Immunosorbents; D009570:Nitriles; D014753:Vinyl Compounds; D012710:Serum Albumin, Bovine; C004985:divinyl benzene; D000181:Acrylonitrile; D005976:Glutaral