Preparation and characterization of monoclonal antibodies to cephalexin-synthesizing enzyme from Acetobacter turbidans.
Eleven monoclonal antibodies against the cephalexin-synthesizing enzyme have been constructed and primarily characterized. These antibodies are all IgG1 type, with medium affinity, and with no enzyme-inhibition effect. They will be utilized as immunoadsorbents to purify their corresponding antigen, the enzyme, in one step.
Yang
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CY
University of California, Davis 95616.
Ryu
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YW
Ryu
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DD
D000150:Acinetobacter / Q000201:enzymology*; D000217:Acyltransferases / Q000276:immunology* / Q000302:isolation & purification; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000915:Antibody Affinity; D006825:Hybridomas / Q000276:immunology; D007074:Immunoglobulin G / Q000096:biosynthesis; D051379:Mice
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D000217:Acyltransferases; C019862:cephalosporin synthesizing enzyme