Xenogenization of tumor cells by transfection with plasmid containing env gene of Friend leukemia virus.
A rat hepatocellular carcinoma cell line (cKDH-8 cl-11) showed decreased tumorigenicity after transfection with an envelope gene derived from a Friend leukemia virus (FV-env gene). FV-env gene product was found by indirect immunofluorescence staining to be expressed on the cell surface of the FV-env gene-transfected cells. The FV-env-transfected cells (FV-env cKDH-8), however, grew well in X-irradiated immunosuppressed rats, indicating that the reduction in tumorigenicity of the transfected cells is based on immunological reaction in the host. The rats which rejected FV-env cKDH-8 cells showed resistance to rechallenge with the parent cKDH-8 cl-11 tumor cells. These results suggest that the FV-env gene product may elicit antitumor immunity against FV-env cKDH-8 cells in a host with a resultant reduction in the tumorigenicity of these cells.
Sugiura
C
C
Laboratory of Pathology, Hokkaido University School of Medicine, Sapporo.
Itaya
T
T
Kondoh
N
N
Oikawa
T
T
Kuzumaki
N
N
Takeichi
N
N
Hosokawa
M
M
Kobayashi
H
H
D000818:Animals; D005622:Friend murine leukemia virus / Q000235:genetics*; D005814:Genes, Viral; D008114:Liver Neoplasms, Experimental / Q000276:immunology*; D009368:Neoplasm Transplantation; D010957:Plasmids; D051381:Rats; D011919:Rats, Inbred Strains; D014162:Transfection; D014407:Tumor Cells, Cultured; D014759:Viral Envelope Proteins / Q000235:genetics*
D014759:Viral Envelope Proteins