A comment on the evaluation of equilibrium constants for alpha-tocopherol interactions with fatty acids by absorbance in the ultraviolet region.
This paper comments on the evaluation of Erin and co-workers (Biochim. Biophys. Acta 774 (1984) 96-102) of equilibrium constants for alpha-tocopherol interactions with fatty acids on the basis of the changes of absorbance in a 200 nm ultraviolet region. It is concluded that the ultraviolet method is inadequate because it is affected by absorption in that region of the solvent, ethanol and fatty acids which they used.
Sugata
S
S
Kyoritsu College of Pharmacy, Tokyo, Japan.
Urano
S
S
Matsushima
Y
Y
Matsuo
M
M
D055598:Chemical Phenomena; D002621:Chemistry; D000431:Ethanol; D005227:Fatty Acids; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet; D014810:Vitamin E
D005227:Fatty Acids; D012996:Solutions; D014810:Vitamin E; D000431:Ethanol