Comparison of gas chromatographic/mass spectrometric and microbiological assays for 5-fluorouracil in plasma.
The concentrations of 5-fluorouracil in 99 plasma samples were determined by both microbiological and gas chromatographic/mass spectrometric assays. The values determined by the two methods were similar (correlation coefficient = 0.90). A regression of the natural logarithms of the concentrations (0.01-94 micrograms ml-1) determined by the two methods gave a line whose slope and intercept were not statistically different (p greater than 0.05) from 1 and 0 respectively. Thus, the microbiological assay has specificity over a sufficient concentration range to serve as a practical routine laboratory method for the determination of 5-fluorouracil.
Min
B H
BH
Garland
W A
WA
Lewinson
T M
TM
Mehta
B M
BM
D001681:Biological Assay; D005472:Fluorouracil / Q000097:blood* / Q000494:pharmacology; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D013291:Streptococcus / Q000187:drug effects
D005472:Fluorouracil