Allocation of the suppressive activity of normal peripheral blood lymphocytes induced by diffusion chamber culture and Con A stimulation to the G2 phase of the cell cycle.
Peripheral blood mononuclear cells stimulated either by diffusion chamber culture or by a high Con A concentration exhibit suppressive activity under conditions where no increase in cell number takes place. Instead an accumulation of large cells is observed which, according to their DNA contents, are classified as cells in the G2 phase of the cell cycle. By elutriation separation the suppressive activity is shown to be confined to this cell cycle phase.
Pachmann
K
K
Pachmann
U
U
Penning
R
R
D000328:Adult; D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface / Q000032:analysis; D002453:Cell Cycle; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D005434:Flow Cytometry; D006801:Humans; D007108:Immune Tolerance; D013601:T-Lymphocytes / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000166:cytology*
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface; D003208:Concanavalin A