Isolation of lambda phage DNA by hydroxylapatite chromatography.
A simple and rapid (1 day) method for preparation of lambda phage DNA was proposed. The method included two main steps: (a) growth and lysis of bacteria containing lambda phage and (b) purification of lambda phage DNA by hydroxylapatite chromatography. The phage DNA prepared by this method was intact and free of RNA, proteins, and bacterial DNA.
Ivanov
I
I
Gigova
L
L
D010582:Bacteriophage lambda / Q000032:analysis*; D002853:Chromatography, Liquid / Q000379:methods*; D004279:DNA, Viral / Q000302:isolation & purification*; D006882:Hydroxyapatites
D004279:DNA, Viral; D006882:Hydroxyapatites