title: Genetic analysis in Streptomyces ambofaciens.
abstract: A chromosomal linkage map of ten markers was established for Streptomyces ambofaciens by the four-factor cross method and allele-gradient analysis. Mutants were obtained by nitrous acid treatment as well as UV mutagenesis. The fertility of crosses was enhanced over 100-fold by pSAM2, a plasmid present in some strains of S. ambofaciens, and over 1000-fold by the conjugative plasmid pIJ303.
authors.affiliation: Laboratoire de Biologie et Génétique Moléculaire, Université de Paris-Sud, Orsay, France.
authors.forename: T
authors.initials: T
authors.lastname: Smokvina
authors.forename: F
authors.initials: F
authors.lastname: Francou
authors.forename: M
authors.initials: M
authors.lastname: Luzzati
mesh_terms: D000483:Alleles; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D003434:Crossing Over, Genetic; D005838:Genotype; D009154:Mutation; D011995:Recombination, Genetic; D013302:Streptomyces / Q000235:genetics*