Functional identification of t-PA in crude and purified systems.
In the present study the activation of glu-plasminogen by fibrin-bound t-PA was determined by integrating spectrophotometric and computer analysis of the reaction. Our aim was to determine the parameters of the activation and to characterize the functional activity of t-PA. Our results indicate that the t-PA present in purified or crude sources such as plasma or culture supernatants, can be specifically identified using the methodology described in this report.
Anglés-Cano
E
E
INSERM U. 143, Hôpital de Bicêtre, France.
Masson
C
C
Grailhe
P
P
Gaussem
P
P
Le Bonniec
B
B
D005337:Fibrin; D005340:Fibrinogen / Q000302:isolation & purification; D006801:Humans; D007700:Kinetics; D010958:Plasminogen / Q000302:isolation & purification; D010959:Tissue Plasminogen Activator / Q000032:analysis*
D005337:Fibrin; D005340:Fibrinogen; D010958:Plasminogen; D010959:Tissue Plasminogen Activator