Retinyl ester synthesis by isolated adult rabbit lung type II cells.
Type II alveolar cells were isolated from adult rabbit lungs and then cultured on monolayers for 16 hours. These cells were then covered with buffered medium containing [3H]-retinol. After 30-120 minutes incubation, the cells were extracted with Hexane: Ethanol and the hexane extract analyzed by HPLC. A linear synthesis of [3H]-retinyl palmitate with time of incubation was demonstrated.
Zachman
R D
RD
University of Wisconsin Perinatal Center, Madison.
Tsao
F H
FH
D000818:Animals; D002478:Cells, Cultured; D004224:Diterpenes; D011650:Pulmonary Alveoli / Q000378:metabolism*; D011817:Rabbits; D000084562:Retinyl Esters; D014801:Vitamin A / Q000031:analogs & derivatives* / Q000096:biosynthesis
D004224:Diterpenes; D000084562:Retinyl Esters; D014801:Vitamin A; C014794:retinol palmitate