title: Antigen presentation in the rat. II. An Ia+ radiosensitive T cell can present antigen to primed Ia- T cells.
abstract: We demonstrated previously the presence of an Ia+ (OX-6+) antigen-presenting cell within the rat T cell fraction that is capable of presenting antigen to antigen-primed OX-6-T cells. This antigen-presenting cell (T-APC) reacted with the monoclonal antibodies W3/25 and W3/13, which is known to react mainly with rat T cells. Further characterization of the T-APC indicated that the cell also reacted with the monoclonal antibody OX-19, which is highly specific for rat T cells. Moreover, the antigen-presenting function of the T-APC was sensitive to treatment with mitomycin C or gamma-irradiation (2000 rad). Under similar conditions, antigen presentation by partially purified dendritic cells or macrophages was totally resistant to these treatments. The antigen-presenting activity of gamma-irradiated T-APC was not reconstituted by the addition of the lymphokines IL 1, IL 2, or Con A supernatants. Although unirradiated T-APC were able to stimulate an MLR response, this function was also sensitive to gamma-irradiation, whereas the MLR-stimulating ability of macrophages and dendritic cells was resistant to gamma-irradiation. These data indicate that Ia+ T cells from the rat are capable of presenting antigen to antigen-primed T lymphocytes and that, in contrast to antigen presentation by macrophages and dendritic cells, the function of T-APC is gamma-radiation sensitive.
authors.forename: M L
authors.initials: ML
authors.lastname: Sopori
authors.forename: D A
authors.initials: DA
authors.lastname: Cohen
authors.forename: S
authors.initials: S
authors.lastname: Cherian
authors.forename: R S
authors.initials: RS
authors.lastname: Perrone
authors.forename: A M
authors.initials: AM
authors.lastname: Kaplan
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000937:Antigen-Antibody Reactions; D000938:Antigen-Presenting Cells / Q000145:classification* / Q000276:immunology / Q000528:radiation effects; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D008198:Lymph Nodes / Q000166:cytology; D008213:Lymphocyte Activation / Q000528:radiation effects; D007959:Lymphocyte Culture Test, Mixed; D010641:Phenotype; D051381:Rats; D011912:Rats, Inbred ACI; D011914:Rats, Inbred BN; D011916:Rats, Inbred F344; D011917:Rats, Inbred Lew; D013601:T-Lymphocytes / Q000145:classification* / Q000276:immunology / Q000528:radiation effects
chemical_list: D000911:Antibodies, Monoclonal; D000949:Histocompatibility Antigens Class II