Modulation of human leukocyte antigen class I expression by gamma interferon in head and neck cancer cell lines.
One possible mechanism explaining the action of interferon (IFN) on squamous cell carcinoma (SqCC) of the head and neck is the modulation of major histocompatibility antigen expression on tumor cells. We tested the ability of gamma interferon (gamma-IFN) to modulate major histocompatibility class I antigens and beta 2-microglobulin (beta 2-M) on two carcinoma cell lines derived from SqCC of the head and neck. Major histocompatibility class I antigens and beta 2-M were detected using a two-step immunochemical stain; antigen expression was quantified using flow cytometry. gamma-IFN increased constitutive antigen expression by as much as five times on both cell lines. Maximum modulation was seen within 72 hours of exposure to gamma-IFN at clinically attainable doses (10 U/mL to 100 U/mL). The presence of gamma-IFN in cell cultures was necessary for continued modulation of surface antigens. These findings suggest a possible mechanism of action and encourage further clinical trials with gamma-IFN.
Houck
J R
JR
Department of Surgery, Milton S. Hershey Medical Center, Pennsylvania State University, Hershey.
Shah
T P
TP
Ohlsson-Wilhelm
B M
BM
Kloszewski
E
E
Conner
B R
BR
D002294:Carcinoma, Squamous Cell / Q000276:immunology* / Q000378:metabolism; D002460:Cell Line; D004306:Dose-Response Relationship, Immunologic; D006258:Head and Neck Neoplasms / Q000276:immunology* / Q000378:metabolism; D015395:Histocompatibility Antigens Class I / Q000032:analysis*; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D007700:Kinetics; D001613:beta 2-Microglobulin / Q000096:biosynthesis
D015395:Histocompatibility Antigens Class I; D001613:beta 2-Microglobulin; D007371:Interferon-gamma