Regulation of enzymes of lysine biosynthesis in Corynebacterium glutamicum.
The regulation of the six enzymes responsible for the conversion of aspartate to lysine, together with homoserine dehydrogenase, was studied in Corynebacterium glutamicum. In addition to aspartate kinase activity, the synthesis of diaminopimelate decarboxylase was also found to be regulated. The specific activity of this enzyme was reduced to one-third in extracts of cells grown in the presence of lysine. Aspartate-semialdehyde dehydrogenase, dihydrodipicolinate synthase, dihydrodipicolinate reductase, and diaminopimelate dehydrogenase were neither influenced in their specific activity, nor inhibited, by any of the aspartate family of amino acids. Homoserine dehydrogenase was repressed by methionine (to 15% of its original activity) and inhibited by threonine (4% remaining activity). Inclusion of leucine in the growth medium resulted in a twofold increase of homoserine dehydrogenase specific activity. The flow of aspartate semialdehyde to either lysine or homoserine was influenced by the activity of homoserine dehydrogenase or dihydrodipicolinate synthase. Thus, the twofold increase in homoserine dehydrogenase activity resulted in a decrease in lysine formation accompanied by the formation of isoleucine. In contrast, repression of homoserine dehydrogenase resulted in increased lysine formation. A similar increase of the flow of aspartate semialdehyde to lysine was found in strains with increased dihydrodipicolinate synthase activity, constructed by introducing the dapA gene of Escherichia coli (coding for the synthase) into C. glutamicum.
Cremer
J
J
Institut für Biotechnologie der Kernforschungsanlage Jülich GmbH, FRG.
Treptow
C
C
Eggeling
L
L
Sahm
H
H
D000596:Amino Acids / Q000494:pharmacology; D001224:Aspartic Acid / Q000378:metabolism / Q000031:analogs & derivatives; D003352:Corynebacterium / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006715:Homoserine Dehydrogenase / Q000378:metabolism; D006801:Humans; D006836:Hydro-Lyases / Q000378:metabolism; D008239:Lysine / Q000096:biosynthesis*
D000596:Amino Acids; D004791:Enzyme Inhibitors; D001224:Aspartic Acid; C006644:aspartic semialdehyde; D006715:Homoserine Dehydrogenase; D006836:Hydro-Lyases; C020040:4-hydroxy-tetrahydrodipicolinate synthase; D008239:Lysine