title: Reutilization of surfactant phosphatidylglycerol and lysophosphatidylcholine by adult rabbits.
abstract: Adult rabbits reutilize the phosphatidylcholine (PC) of surfactant much less efficiently than developing rabbits (22% vs. 95%). Comparisons of reutilization efficiency of other components of surfactant in adult rabbits have not been determined. We injected adult rabbits intratracheally with [3H]dipalmitoylphosphatidylcholine (DPPG) mixed with [14C]lysophosphatidylcholine (lysoPC) and natural surfactant or [14C]DPPC mixed with [3H]dipalmitoylphosphatidylglycerol (DPPG) and natural surfactant. Recovery in the alveolar wash and lamellar bodies of labelled DPPC, lysoPC and DPPG was determined at different times after injection. By plotting the ratio of [3H]DPPG to [14C]DPPC in the alveolar wash versus time after injection we found that phosphatidylglycerol was reutilized with an efficiency of only 0-7% which was much less than the reutilization of PC in these animals. At early times after injection, adult rabbits injected with [14C]lysoPC had a ratio of [14C]PC in their alveolar wash to lamellar bodies that was larger than 1.0. By comparison, 3-day old rabbits injected intratracheally with [14C]lysoPC had a ratio of [14C]PC in alveolar wash to lamellar bodies less than 1.0 at the earliest times measurable. Thus adult rabbits demonstrate a pathway for accumulation of PC in their alveolar space prior to its appearance in lamellar bodies. This was not detected in developing rabbits. As in developing rabbits, adult rabbits reutilize the phosphatidylglycerol of surfactant less efficiently than the PC of surfactant.
authors.affiliation: Yale University, Department of Pediatrics, New Haven, CT 06510.
authors.forename: H C
authors.initials: HC
authors.lastname: Jacobs
authors.forename: D M
authors.initials: DM
authors.lastname: Lima
authors.forename: J M
authors.initials: JM
authors.lastname: Fiascone
authors.forename: M R
authors.initials: MR
authors.lastname: Mercurio
mesh_terms: D000375:Aging; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000032:analysis; D005260:Female; D008168:Lung / Q000378:metabolism*; D008244:Lysophosphatidylcholines / Q000493:pharmacokinetics*; D010715:Phosphatidylglycerols / Q000493:pharmacokinetics*; D011663:Pulmonary Surfactants / Q000493:pharmacokinetics*; D011817:Rabbits
chemical_list: D008244:Lysophosphatidylcholines; D010715:Phosphatidylglycerols; D011663:Pulmonary Surfactants