Protective effect of taurine on the irregular beating pattern of cultured myocardial cells induced by high and low extracellular calcium ion.
Abnormal beating patterns were induced in spontaneously contracting cultured embryonic mouse myocardial cells either by elevating or by lowering extracellular calcium. At low calcium (0.4 mM), the number of beating cells and beating rate decreased while the number of arrhythmic cells increased. By contrast, at high calcium (20 mM), the number of beating cells decreased while beating rate and the number of arrhythmic cells increased. Addition of taurine (20 mM) to the medium attenuated the response to varying calcium; the taurine effect appeared to be specific since neither taurine analog tested (beta-alanine nor glycine) provided much protection against these abnormalities. The protective effect of taurine also appeared to differ from that of verapamil, which was effective only in decreasing beating rate in the high calcium condition. Uptake of 14C-taurine by the cells was higher at both low and high extracellular calcium when compared to the normal calcium (2 mM) concentration. The results raise the possibility that the protective effect of taurine on beating abnormalities caused by low or high calcium is related to taurine uptake.
Takahashi
K
K
Third Department of Internal Medicine, Osaka University Medical School, Japan.
Azuma
J
J
Awata
N
N
Sawamura
A
A
Kishimoto
S
S
Yamagami
T
T
Kishi
T
T
Harada
H
H
Schaffer
S W
SW
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002118:Calcium / Q000494:pharmacology*; D002478:Cells, Cultured; D004622:Embryo, Mammalian; D005998:Glycine / Q000494:pharmacology; D006321:Heart / Q000187:drug effects / Q000502:physiology*; D006339:Heart Rate / Q000187:drug effects*; D007700:Kinetics; D051379:Mice; D013654:Taurine / Q000378:metabolism / Q000494:pharmacology*; D014700:Verapamil / Q000494:pharmacology; D015091:beta-Alanine / Q000494:pharmacology
D015091:beta-Alanine; D013654:Taurine; D014700:Verapamil; D002118:Calcium; D005998:Glycine