title: Helper T cell requirements for T15 idiotype expression on phosphorylcholine-specific antibodies.
abstract: The requirement for idiotype-specific T cells was investigated in the T15 idiotype-dominant T cell-dependent response of unprimed BALB/c and (BALB/c X C57BL/6)F1 B cells to phosphorylcholine (PC). It was first demonstrated that cloned keyhole limpet hemocyanin (KLH)-specific, major histocompatibility complex (MHC)-restricted T helper (Th) cells as well as heterogeneous KLH-primed Th populations were capable of generating PC-specific antibody responses in T-depleted unprimed B cell populations cultured in the presence of PC-KLH. The PC-binding antibody responses generated under these conditions were indistinguishable when assayed for carrier-hapten linkage requirements, immunoglobulin isotype (predominantly IgM) or PC affinity. Further, it was observed that the PC-binding antibodies which were generated in the presence of these two T cell populations expressed equivalently high levels of T15 idiotype. Assaying antibody and idiotype by either enzyme-linked immunosorbent assay or plaque-forming cell assay yielded similar results. Since monoclonal MHC-restricted, KLH-specific Th cells presumably lack any additional T cell populations, these results argue against an absolute requirement for anti-idiotypic Th cells in the generation of T15-dominant antibody responses.
authors.forename: K S
authors.initials: KS
authors.lastname: Hathcock
authors.forename: J J
authors.initials: JJ
authors.lastname: Kenny
authors.forename: R J
authors.initials: RJ
authors.lastname: Hodes
mesh_terms: D000818:Animals; D000917:Antibody Formation; D000918:Antibody Specificity; D001402:B-Lymphocytes / Q000276:immunology*; D002794:Choline / Q000031:analogs & derivatives*; D002999:Clone Cells / Q000276:immunology; D006433:Hemocyanins / Q000276:immunology; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D008211:Lymphocyte Cooperation; D051379:Mice; D008815:Mice, Inbred Strains; D010767:Phosphorylcholine / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*
chemical_list: D007130:Immunoglobulin Idiotypes; D010767:Phosphorylcholine; D006433:Hemocyanins; D002794:Choline