title: Determination of 4,4'-methylenebis(2-chloroaniline) in urine by liquid chromatography with ion-paired solid-phase extraction and electrochemical detection.
abstract: This highly specific and sensitive method for measuring urinary 4,4'-methylenebis(2-chloroaniline) (MBOCA) involves liquid chromatography with electrochemical detection. Before chromatography, urine samples are prepared by ion-paired solid-phase extraction on a disposable octadecylsilica column with acidic methanol solution containing 1-heptanesulfonic acid. This enhances the specificity of the method. Mean overall recovery ranged from 97.1% to 99.5% at added MBOCA concentrations of 20 and 100 micrograms/L in urine. Sensitivity for urine was 1 microgram/L. The intra-assay CV was 2.2% at a MBOCA concentration of 100 micrograms/L. We believe that this method is acceptable for routine measurement of MBOCA in urine from individuals exposed to this industrial chemical.
authors.affiliation: Department of Hygiene & Preventive Medicine, Osaka University Medical School, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Okayama
authors.forename: Y
authors.initials: Y
authors.lastname: Ichikawa
authors.forename: M
authors.initials: M
authors.lastname: Yoshida
authors.forename: I
authors.initials: I
authors.lastname: Hara
authors.forename: K
authors.initials: K
authors.lastname: Morimoto
mesh_terms: D001559:Benzhydryl Compounds / Q000652:urine*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004563:Electrochemistry; D006801:Humans; D008753:Methylenebis(chloroaniline) / Q000652:urine*
chemical_list: D001559:Benzhydryl Compounds; D008753:Methylenebis(chloroaniline)