Effect of 7,12-dimethylbenz(a)anthracene administration on hepatic drug metabolizing enzymes in female rats fed corn, primrose, and menhaden oils.
Female rats receiving a diet containing 20% menhaden oil beginning at 10 weeks of age and continuing for 13 weeks had hepatic benzo(a)pyrene [B(a)P] hydroxylase activity significantly higher than similar rats fed diets containing 20% corn oil or 20% oil of evening primrose. Compared to microsomes recovered from rats fed the corn oil diet, a significant increase in microsomal cytochrome P-450 content along with an increase in the activity of cytochrome P-450 mediated ethoxycoumarin O-dealkylase was evident in rats fed menhaden oil. Glutathione S-transferase activity of the cytosol of hepatocytes was increased twofold by the feeding of 20% menhaden oil, compared with the feeding of corn or primrose oil. Administration of 7,12-dimethylbenz(a)anthracene (DMBA) 21 days before instituting the diets enhanced B(a)P hydroxylase in all animals, with the activity greatest in those fed the menhaden oil. DMBA also caused a significant increase in ethoxycoumarin O-dealkylase in rats fed menhaden oil.
Wade
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College of Pharmacy, Department of Pharmacology and Toxicology, University of Georgia, Athens 30602.
Dharwadkar
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Bunce
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Abou el-Ela
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D015127:9,10-Dimethyl-1,2-benzanthracene / Q000494:pharmacology*; D000818:Animals; D003314:Corn Oil / Q000494:pharmacology; D004042:Dietary Fats, Unsaturated / Q000494:pharmacology*; D005228:Fatty Acids, Essential / Q000494:pharmacology; D005260:Female; D005395:Fish Oils / Q000494:pharmacology; D008041:Linoleic Acids; D008099:Liver / Q000201:enzymology*; D006899:Mixed Function Oxygenases / Q000378:metabolism*; D031665:Oenothera biennis; D010938:Plant Oils; D051381:Rats; D011919:Rats, Inbred Strains; D017965:gamma-Linolenic Acid
D004042:Dietary Fats, Unsaturated; D005228:Fatty Acids, Essential; D005395:Fish Oils; D008041:Linoleic Acids; D010938:Plant Oils; C033266:Menhaden oil; C028498:evening primrose oil; D015127:9,10-Dimethyl-1,2-benzanthracene; D017965:gamma-Linolenic Acid; D003314:Corn Oil; D006899:Mixed Function Oxygenases