title: Effects of synthetic analogues of platelet-activating factor (PAF) on fibrinolytic activity in the rat.
abstract: PAF and structural analogues were investigated for their in vivo effects on fibrinolytic activity (FA) in the rat. The i.v. administration of 1 and 4 micrograms/kg PAF caused a dose-dependent increase in FA which was shown to be attributable to the release of tissue-type plasminogen activator (t-PA). 1-O-hexadecyl-2-O-ethyl-glycero-3-phosphoric acid-2'-N-propargyl-N,N'- dimethylammoniumethyl ester (I) and 1-O-hexadecyl-2-(n-propyl)-propanediol-3-phosphocholine (II) increased FA according to their proaggregatory activity. In contrast, 1-O-hexadecyl-2-O-ethyl-rac-glycero-3-phosphoric acid-5'-trimethylammoniumpentyl ester (III), a competitive antagonist of PAF, reduced the PAF induced increase of FA by 30%. Under identical conditions BN 52021 given at 1 and 10 micrograms/kg amounts diminished the effect of PAF by 47 and 77%, respectively. These results indicate that the PAF induced PA release in vivo is receptor mediated.
authors.affiliation: Institut für Pathologische Biochemie, Medizinische Akademie, Erfurt, GDR.
authors.forename: B
authors.initials: B
authors.lastname: Hofmann
authors.forename: U
authors.initials: U
authors.lastname: Meisgeier
authors.forename: P
authors.initials: P
authors.lastname: Kertscher
authors.forename: G
authors.initials: G
authors.lastname: Ostermann
mesh_terms: D000818:Animals; D005342:Fibrinolysis / Q000187:drug effects*; D010743:Phospholipids / Q000138:chemical synthesis; D010972:Platelet Activating Factor / Q000494:pharmacology*; D051381:Rats; D013329:Structure-Activity Relationship; D010959:Tissue Plasminogen Activator / Q000378:metabolism
chemical_list: D010743:Phospholipids; D010972:Platelet Activating Factor; D010959:Tissue Plasminogen Activator