Protein-specific helper T-lymphocyte formation initiated by dendritic cells.
Antibody responses to hapten-polypeptide conjugates require peptide-specific helper T cells. The latter can be primed in tissue culture by providing small numbers of dendritic cells. Primed, irradiated helper T cells then induce B-cell growth and differentiation in the apparent absence of dendritic cells. Both stages of the antibody response--the induction of helper T lymphoblasts by dendritic cells and the delivery of help from T to B cell--occur in discrete cell aggregates that can be isolated by velocity sedimentation. If helper T blasts revert to smaller "memory" lymphocytes, dendritic cells again are needed to initiate the antibody response.
Inaba
K
K
Steinman
R M
RM
D000818:Animals; D000917:Antibody Formation; D000938:Antigen-Presenting Cells / Q000502:physiology*; D001402:B-Lymphocytes / Q000276:immunology; D002448:Cell Adhesion; D006241:Haptens / Q000276:immunology; D007156:Immunologic Memory; D066298:In Vitro Techniques; D008264:Macrophages / Q000502:physiology; D051379:Mice; D011506:Proteins / Q000276:immunology*; D013154:Spleen / Q000166:cytology* / Q000502:physiology; D013601:T-Lymphocytes / Q000276:immunology; D006377:T-Lymphocytes, Helper-Inducer / Q000502:physiology*
D006241:Haptens; D011506:Proteins