Dose-response aggregometry in maternal/neonatal platelets.
The aggregation of platelets collected from maternal/neonatal pairs (n = 240) at the time of childbirth, was studied in response to multiple doses of ADP, collagen, arachidonic acid and ristocetin. Similar responses were obtained from healthy nonpregnant adult controls for comparison. The lag phase, slope of the aggregation curves as well as maximum aggregation (MA%) were recorded and analysed. Neonatal and adult platelets exhibited more enhanced responses to decreasing doses of ADP, arachidonic acid and ristocetin, than maternal platelets. These enhanced responses were exhibited more consistently in the slopes of the aggregation curves than in MA%. Although neonatal platelets have shown longer lag phase in their responses to collagen, the rate of the aggregation reaction was significantly faster than maternal platelets, with no differences in MA%. These results contradict many previous reports suggesting impaired aggregation responses of neonatal platelets to these agonist. The possible reasons for these contradictions were discussed.
Gader
A M
AM
Department of Physiology, College of Medicine & King Khaled University Hospital, Riyadh, Saudi Arabia.
Bahakim
H
H
Jabbar
F A
FA
Lambourne
A L
AL
Gaafar
T H
TH
Edrees
Y B
YB
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000293:Adolescent; D000328:Adult; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000494:pharmacology; D003094:Collagen / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D007231:Infant, Newborn / Q000097:blood*; D008431:Maternal-Fetal Exchange; D008875:Middle Aged; D010974:Platelet Aggregation; D011247:Pregnancy; D012310:Ristocetin / Q000494:pharmacology
D001095:Arachidonic Acids; D012310:Ristocetin; D016718:Arachidonic Acid; D000244:Adenosine Diphosphate; D003094:Collagen