Immunochemical studies on pneumococcal type 37 capsular polysaccharide.
Pneumococcal type 37 capsular polysaccharide was obtained free of contaminants by affinity chromatography on Con-A, wheat germ agglutinin, Maclura pomifera lectin and HOPC-8 mouse myeloma protein affinity columns. The immunochemical reactivity of native and periodate oxidized borohydride reduced type 37 polysaccharide antigen with polyclonal rabbit and monoclonal mouse anti-Pn37 hybridoma antibodies was studied by quantitative precipitation. Quantitative hapten inhibition studies, employing the isomeric series of alpha- and beta-(1----2), (1----3), (1----4) and (1----6)-linked glucobioses as competitive inhibitors of antibody precipitation establish a specificity for anti-Pn37 antibody directed at least in part, against the Glc beta(1----2) Glc (sophorosyl) unit. A high mol. wt, D-glucose containing polysaccharide antigen, cross-reactive with rabbit anti-Pn37 is reported which was found to occur in the culture medium of 7 of 19 strains of Actinomyces examined.
Allen
P Z
PZ
Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, NY 14642.
Bowen
W H
WH
D000818:Animals; D001667:Binding, Competitive; D055598:Chemical Phenomena; D002621:Chemistry; D002846:Chromatography, Affinity; D003429:Cross Reactions; D006241:Haptens / Q000276:immunology; D005779:Immunodiffusion; D011135:Polysaccharides, Bacterial / Q000032:analysis* / Q000276:immunology* / Q000302:isolation & purification
D006241:Haptens; D011135:Polysaccharides, Bacterial; C058381:pneumococcal polysaccharide, type 37