Cross-linking of native myosin forms oligomers of myosin heavy chain dimers.
Chemical cross-linking of native myosin in 0.6 M NaCl with p-phenylene bis maleimide or glutaraldehyde resulted in rapid formation of myosin heavy chain dimers and their oligomers. Monomers and odd-number oligomers disappeared after the prolonged treatment with these reagents. When denatured myosin was cross-linking, myosin heavy chain monomers and odd-number oligomers remained after the prolonged treatment, although dimers and their even-number oligomers were abundant. For high molecular weight markers, myosin heavy chain oligomers formed from denatured myosin with glutaraldehyde or p-phenylene bis maleimide are recommended.
Hu
D H
DH
Department of Biology, Faculty of Science, Chiba University.
Kimura
S
S
Maruyama
K
K
D003432:Cross-Linking Reagents / Q000494:pharmacology; D005976:Glutaral / Q000494:pharmacology; D008301:Maleimides / Q000494:pharmacology; D009218:Myosins / Q000302:isolation & purification*; D011489:Protein Denaturation
D003432:Cross-Linking Reagents; D008301:Maleimides; C011328:N,N'-4-phenylenedimaleimide; D009218:Myosins; D005976:Glutaral