Structure elucidation of mitomycin C metabolites. I. The applicability of various mass spectrometric techniques in metabolic studies on mitomycin C.
Knowledge of the metabolism of the anticancer drug mitomycin C (MMC) is necessary in order to optimize the administration schemes of this drug. The present study concentrates on the metabolism of MMC in liver homogenates. Various mass spectrometric methods have been investigated for their usefulness in the metabolic studies on MMC by comparing the mass spectral data. Data from electron impact, direct probe chemical ionization, direct chemical ionization, field desorption and thermospray ionization have been obtained. One of the soft ionization methods, i.e. direct chemical ionization, has been applied to the detection of MMC in a spiked piglet liver sample. It appears to be necessary to enhance the selectivity of the method by using tandem mass spectrometry, owing to the high biological background.
Heeremans
C E
CE
Division of Analytical Chemistry, Center for Bio-Pharmaceutical Sciences, Leiden, The Netherlands.
van der Hoeven
R A
RA
Niessen
W M
WM
Tjaden
U R
UR
LaVos
G F
GF
van der Greef
J
J
D000818:Animals; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism*; D016685:Mitomycin; D008937:Mitomycins / Q000378:metabolism*; D013552:Swine
D008937:Mitomycins; D016685:Mitomycin