Intracellular pathway of interleukin-2 receptors studied using immunogold electron microscopy.
The fate of internalized interleukin-2 receptors (IL-2R) on proliferating T cell blasts and Lyt-2-/L3T4- thymocytes was studied by immunogold electron microscopy. Immediately following labeling, IL-2R were found in coated pits and with time there was sequential appearance of gold particles in smooth vesicles or endosomes, multivesicular bodies and lysosomes. Thus the IL-2R appears to be a member of cell surface receptors internalized by endocytosis via coated pits.
McCrae
C
C
Department of Experimental Pathology, John Curtin School of Medical Research, Canberra, Australia.
Maxwell
L
L
Ceredig
R
R
D000818:Animals; D003034:Coated Pits, Cell-Membrane / Q000276:immunology / Q000648:ultrastructure; D004705:Endocytosis / Q000276:immunology; D008213:Lymphocyte Activation; D051379:Mice; D016253:Microscopy, Immunoelectron; D015375:Receptors, Interleukin-2 / Q000378:metabolism*; D013601:T-Lymphocytes / Q000276:immunology* / Q000648:ultrastructure
D015375:Receptors, Interleukin-2