title: Fat tissue: a steroid reservoir and site of steroid metabolism.
abstract: Sex steroid concentrations and 17 beta-hydroxy-steroid dehydrogenase and aromatase activities were determined in fat tissue removed at surgery or, in order to allow comparisons in different sites, postmortem. Except for dehydroepiandrosterone (DHEA) sulfate (DHEAS), there existed a positive tissue/plasma gradient for all steroids studied (testosterone, androstenedione, DHEA, androstenediol, estrone, and estradiol), suggesting androgen uptake and estrogen synthesis in situ. Androgen concentrations did not vary according to site of origin of fat tissue, except that the DHEAS concentration was significantly lower in abdominal sc and omental fat than in breast, pericardial, or sc pubic fat. Tissue androgen concentrations were positively correlated with their plasma concentrations, but tissue and plasma estrogen concentrations were not correlated. All tissue steroid concentrations, with the exception of estradiol in men, decreased with age. Aromatase activity [androstenedione----estrone; mean maximum velocity, 7.4 +/- 3.7 (+/- SD) fmol estrone/mg protein . h] did not vary between sexes or with site of origin of fat tissue. 17 beta-Hydroxysteroid dehydrogenase activity (estradiol----estrone, mean maximum velocity 9.8 +/- 5.4 pmol/mg protein . h) was higher in fat from women than in that from men, higher in premenopausal than in postmenopausal women, and higher in omental than in sc fat. Its activity was noncompetitively inhibited in vitro by DHEA and DHEAS in near-physiological concentrations, and the enzyme activity was inversely correlated (P less than 0.001) with the tissue DHEA and DHEAS concentrations. We conclude that fat tissue is an important steroid hormone reservoir, that it is the site of active aromatase and 17 beta-hydroxysteroid dehydrogenase, and that tissue DHEA(S) may have a modulating effect on tissue estrogen production.
authors.forename: J P
authors.initials: JP
authors.lastname: Deslypere
authors.forename: L
authors.initials: L
authors.lastname: Verdonck
authors.forename: A
authors.initials: A
authors.lastname: Vermeulen
mesh_terms: D015067:17-Hydroxysteroid Dehydrogenases / Q000378:metabolism; D000273:Adipose Tissue / Q000201:enzymology / Q000378:metabolism*; D000328:Adult; D000368:Aged; D015114:Androstenediol / Q000378:metabolism; D000735:Androstenedione / Q000378:metabolism; D001141:Aromatase / Q000378:metabolism; D001665:Binding Sites; D003687:Dehydroepiandrosterone / Q000031:analogs & derivatives / Q000378:metabolism; D019314:Dehydroepiandrosterone Sulfate; D004958:Estradiol / Q000378:metabolism; D004967:Estrogens / Q000096:biosynthesis; D004970:Estrone / Q000378:metabolism; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D013256:Steroids / Q000378:metabolism*; D013739:Testosterone / Q000378:metabolism
chemical_list: D004967:Estrogens; D013256:Steroids; D004970:Estrone; D013739:Testosterone; D000735:Androstenedione; D003687:Dehydroepiandrosterone; D004958:Estradiol; D019314:Dehydroepiandrosterone Sulfate; D015114:Androstenediol; D015067:17-Hydroxysteroid Dehydrogenases; D001141:Aromatase