Expression of a gene for glucan-binding protein from Streptococcus mutans in Escherichia coli.
The structural gene for a glucan-binding protein (GBP) of Streptococcus mutans has been inserted into a bacteriophage lambda vector and expressed in Escherichia coli K12. Lysates of E. coli infected with the recombinant phage contain an antigenic protein of the same size as S. mutans GBP. The GBP synthesized in E. coli can be affinity-purified on immobilized glucan and antiserum raised against it has been shown to precipitate fructosyltransferase activity from S. mutans.
Russell
R R
RR
Coleman
D
D
Dougan
G
G
D000942:Antigens, Bacterial / Q000276:immunology; D010582:Bacteriophage lambda / Q000235:genetics; D002352:Carrier Proteins / Q000235:genetics* / Q000276:immunology; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics* / Q000276:immunology; D005796:Genes; D005798:Genes, Bacterial; D006602:Hexosyltransferases / Q000276:immunology; D007122:Immunoelectrophoresis; D037102:Lectins; D013295:Streptococcus mutans / Q000235:genetics* / Q000276:immunology; D001426:Bacterial Proteins
D000942:Antigens, Bacterial; D002352:Carrier Proteins; D006602:Hexosyltransferases; D037102:Lectins; C037937:glucan-binding proteins; C021451:inulosucrase; D001426:Bacterial Proteins