Characterization of the low mol. wt zinc-binding ligand from rat small intestine by comparison to the organic zinc-binding ligands.
1. 65Zn complexes of picolinate (PA), citrate (CA), L-histidine (L-his), arachidonic acid (AA) or low mol. wt zinc-binding ligand from rat intestine (LMW-ZBL) gave 65Zn eluting peak fraction numbers of 53, 53, 56, 59 and 59 respectively, in a Sephadex G-75 column chromatography. 2. The 65Zn eluting peak fraction numbers with CA, L-his, PA, prostaglandin (PG)E2, AA, no ligand, arachidonate (AT) or LMW-ZBL were 49, 50, 54, 55, 58, 64, 75 and 76 respectively in a Sephadex G-25 column chromatography. 3. In a Sephadex G-15 column chromatography, the 65Zn eluting peak fraction numbers with CA, PGE2, AA, L-his, LMW-ZBL or PA were 49, 50, 51, 52, 52 and 55 respectively. 4. The LMW-ZBL in rat small intestine appears to be an AA-like substance.
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Department of Pediatrics, University of California School of Medicine, Los Angeles 90024.
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D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism; D002352:Carrier Proteins / Q000378:metabolism*; D002951:Citrates / Q000378:metabolism; D019343:Citric Acid; D015232:Dinoprostone / Q000378:metabolism; D006639:Histidine / Q000378:metabolism; D066298:In Vitro Techniques; D007421:Intestine, Small / Q000378:metabolism*; D008970:Molecular Weight; D010848:Picolinic Acids / Q000378:metabolism; D051381:Rats; D015032:Zinc / Q000378:metabolism*
D001095:Arachidonic Acids; D002352:Carrier Proteins; D002951:Citrates; D010848:Picolinic Acids; C017800:zinc-binding protein; D016718:Arachidonic Acid; D019343:Citric Acid; D006639:Histidine; D015032:Zinc; D015232:Dinoprostone; C030614:picolinic acid