[In situ characterization of immunologically competent cells during the growth of a transplanted murine sarcoma].
To characterize the immunocompetent cell populations which infiltrate a transplantable MCA-induced sarcoma and to study their modifications during tumoral growth, we used fluorescent antibodies to the cell membrane antigens Lyt-1, Lyt-2 and Asialo GM1. Early after tumor graft, an accumulation of cells bearing the Asialo-GM1 antigen was observed; this population corresponds mostly to NK cells. Simultaneously, a macrophage infiltration, identified by a cytochemical method, was seen. After this period, an accumulation of cells bearing the Lyt-1 antigen was observed; Lyt-2 positive cells were detected continuously during experimental period.
De Graef-Lovens
D
D
Schaaf-Lafontaine
N
N
Boniver
J
J
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D003602:Cytotoxicity, Immunologic; D005354:Fibrosarcoma / Q000276:immunology* / Q000473:pathology; D007694:Killer Cells, Natural / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology*; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009368:Neoplasm Transplantation; D013601:T-Lymphocytes / Q000276:immunology; D013997:Time Factors
Caractérisation in situ des cellules immunologiquement compétentes au cours de la croissance d'un sarcome murin transplanté.