title: A stable isotope assay for phenprocoumon and its metabolites.
abstract: A gas chromatographic/mass spectrometric assay for quantifying phenprocoumon and its 4'-, 6-, 7- and 8-hydroxy metabolites in microsomal preparations is described. This assay which uses deuterium-labeled analogs of the phenprocoumon metabolites as internal standards has a lower limit of quantitation of 20 ng ml-1. Diazomethane is used to derivatize both metabolites and parent compound yielding along with the expected 4-methoxy derivative a minor amount of the 2-methoxychromone. Resolution of the methylated metabolites is accomplished by capillary gas chromatography.
authors.forename: L D
authors.initials: LD
authors.lastname: Heimark
authors.forename: W F
authors.initials: WF
authors.lastname: Trager
mesh_terms: D015110:4-Hydroxycoumarins / Q000032:analysis*; D000818:Animals; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D007668:Kidney / Q000166:cytology / Q000378:metabolism; D008168:Lung / Q000166:cytology / Q000378:metabolism; D008861:Microsomes / Q000032:analysis / Q000378:metabolism; D008862:Microsomes, Liver / Q000032:analysis / Q000378:metabolism; D010644:Phenprocoumon / Q000032:analysis* / Q000378:metabolism; D051381:Rats
chemical_list: D015110:4-Hydroxycoumarins; D010644:Phenprocoumon