[Immunological study on localized intrahepatic injection of Adriamycin--experimental study in rats].
Using Charles River-SD 7-week-old rats, a study was carried out on the immunological changes resulting from localized intrahepatic injection of Adriamycin (ADM). The subjects were categorized into groups injected with either saline solution, ADM-0.5mg/kg, ADM-1.0mg/kg or ADM-2.0mg/kg. Measurements were undertaken prior to injection, on the first day and on the fifth day after injection through cardiac blood sampling to determine the absolute counts of T-cells, B-cells, helper T-cells, and suppressor T-cells. On comparing these values in the saline group to the ADM groups prior to the ADM groups prior to the first day. By the fifth day, however, significant differences were evident between changes which had occurred in the two groups. When changes in the saline group and the ADM-0.5 group were studied prior to localized intrahepatic injection on the fifth day, there no significant differences. The ADM-1.0 group and the ADM-2.0 group showed a decline in T-cells (P less than 0.05) with no changes in B-cells, Th-cells or Ts-cells. As shown above, when the liver was injected with saline, no immunological changes were recognized. On the other hand, reduction in T-cells was ascertained when ADM was injected.
Takada
T
T
Yasuda
H
H
Uchiyama
K
K
Hasegawa
H
H
D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects*; D004317:Doxorubicin / Q000008:administration & dosage / Q000494:pharmacology*; D007267:Injections; D007958:Leukocyte Count; D008099:Liver; D051381:Rats; D013601:T-Lymphocytes / Q000187:drug effects*; D006377:T-Lymphocytes, Helper-Inducer / Q000187:drug effects
D004317:Doxorubicin