Cytogenetic evidence of clonal proliferation of leukemic progenitor cells from patients with acute promyelocytic leukemia (APL).
We have studied in-vitro growth of leukemic progenitor cells (L-CFU) in ten patients with acute promyelocytic leukemia (APL). All patients showed consistently an extraordinarily high incidence of cluster formation under the stimulation of human placental conditioned medium (HPCM) and/or phytohemagglutinin-stimulated leukocyte conditioned medium (PHA-LCM). Cytogenetic analyses of clusters or colonies disclosed the presence of a 15;17 translocation. These findings may represent the close relationship between specific chromosomal aberration, t(15;17), and the growth pattern of L-CFU of APL in vitro.
Yokota
S
S
Third Department of Internal Medicine, Kyoto Prefectural University of Medicine, Japan.
Sonoda
Y
Y
Okuda
T
T
Maekawa
T
T
Nishigaki
H
H
Misawa
S
S
Takino
T
T
Abe
T
T
D000328:Adult; D000368:Aged; D002884:Chromosomes, Human, Pair 15; D002886:Chromosomes, Human, Pair 17; D005260:Female; D006801:Humans; D007621:Karyotyping; D015470:Leukemia, Myeloid, Acute / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D013234:Stem Cells / Q000473:pathology; D014178:Translocation, Genetic