Influence of poly(ADP-ribose) synthesis inhibitors on the repair of sublethal and potentially lethal damage in gamma-irradiated mammalian cells.
The influence of poly(ADP-ribose) synthesis inhibitors on mammalian cell radiosensitivity was investigated. Four different inhibitors were studied: 3-methoxybenzamide, 3-aminobenzamide, 6-aminonicotinamide and nicotinamide. When exponentially growing or plateau phase cells are incubated before irradiation with non-toxic concentrations of these compounds, their radiosensitivity is enhanced except in the case of nicotinamide. The poly(ADP-ribose) inhibitors do not modify the repair of sublethal damage, but totally suppress the repair of potentially lethal damage, as shown by the survival of CHO cells and of a human osteosarcoma cell line.
Huet
J
J
Laval
F
F
D015120:6-Aminonicotinamide / Q000494:pharmacology; D000818:Animals; D001549:Benzamides / Q000494:pharmacology; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003037:Cobalt Radioisotopes; D006224:Cricetinae; D004260:DNA Repair / Q000187:drug effects*; D005720:Gamma Rays; D006801:Humans; D066298:In Vitro Techniques; D009536:Niacinamide / Q000494:pharmacology; D009702:Nucleoside Diphosphate Sugars / Q000037:antagonists & inhibitors*; D011064:Poly Adenosine Diphosphate Ribose / Q000037:antagonists & inhibitors*; D011836:Radiation Tolerance
D001549:Benzamides; D003037:Cobalt Radioisotopes; D009702:Nucleoside Diphosphate Sugars; D009536:Niacinamide; D011064:Poly Adenosine Diphosphate Ribose; D015120:6-Aminonicotinamide; C025160:3-aminobenzamide; C025159:3-methoxybenzamide