The six amino-terminal amino acids of p60src are sufficient to cause myristylation of p21v-ras.
We have used oligonucleotide-directed mutagenesis to replace the N-terminal amino acids of p21v-ras with residues which mimic the amino terminus of p60v-src. p21v-ras protein possessing only the first five amino acids of p60src was not myristylated, while substitution of residue 6 (serine) produced a protein p21(GSSKS) which incorporated [3H]myristic acid that was stable to hydroxylamine, sensitive to inhibitors of protein synthesis, and found in both the normally nonacylated precursor and mature forms of p21(GSSKS). This defines the minimum framework of the p60v-src myristylation signal (glycine 2 and serine 6) and identifies serine 6 as a crucial part of that signal for myristylation of a protein in vivo.
Buss
J E
JE
La Jolla Cancer Research Foundation, California 92037.
Der
C J
CJ
Solski
P A
PA
D000595:Amino Acid Sequence; D001483:Base Sequence; D006251:Harvey murine sarcoma virus / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation; D019814:Myristic Acid; D009227:Myristic Acids / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000235:genetics*; D015689:Oncogene Protein p21(ras); D015688:Oncogene Protein pp60(v-src); D009856:Oncogene Proteins, Viral / Q000235:genetics* / Q000378:metabolism; D011505:Protein-Tyrosine Kinases / Q000235:genetics*; D012191:Retroviridae Proteins / Q000235:genetics* / Q000378:metabolism
D009227:Myristic Acids; D009363:Neoplasm Proteins; D009856:Oncogene Proteins, Viral; D012191:Retroviridae Proteins; D019814:Myristic Acid; D011505:Protein-Tyrosine Kinases; D015688:Oncogene Protein pp60(v-src); D015689:Oncogene Protein p21(ras)