Acid phosphatase activity of virulent and avirulent clones of Leishmania donovani promastigotes.
Virulent and avirulent clones of Leishmania donovani promastigotes were examined for their acid phosphatase activity. The acid phosphatase activity of whole-cell lysates of virulent clones was 1.5 to 2.0 times higher than that of avirulent clones. Pellet fractions (260,000 x g, 30 min) from sonicated promastigotes of a virulent clone and an avirulent clone contained 60 and 40% of the total enzyme activity, respectively. Membrane-bound acid phosphatase was extracted with Triton X-100 from the pellet. This membrane-bound phosphatase activity was 2.4-fold higher in virulent organisms than in avirulent organisms. The membrane acid phosphatase exhibited two distinct bands on polyacrylamide gels stained for enzyme activity. One diffuse, faster-migrating band showed identical electrophoretic mobility in both virulent and avirulent clones, although a higher enzymatic activity was observed with the extract from virulent cells. In contrast, a slower-migrating band was different between the two clones in the mobility. These results suggest that membrane-bound acid phosphatase was quantitatively and qualitatively different between virulent and avirulent promastigotes of L. donovani. In addition, virulent cells produced a relatively high level of acid phosphatase throughout the growth in culture.
Katakura
K
K
Department of Parasitology, Jikei University School of Medicine, Tokyo, Japan.
Kobayashi
A
A
D000135:Acid Phosphatase / Q000032:analysis / Q000378:metabolism*; D000818:Animals; D002462:Cell Membrane / Q000201:enzymology; D002999:Clone Cells; D004591:Electrophoresis, Polyacrylamide Gel; D007893:Leishmania donovani / Q000201:enzymology* / Q000254:growth & development / Q000472:pathogenicity; D011092:Polyethylene Glycols / Q000494:pharmacology; D013997:Time Factors
D011092:Polyethylene Glycols; D000135:Acid Phosphatase