title: Circadian variations of autologous mixed lymphocyte reactions and endogenous cortisol.
abstract: The degree of proliferation of human T cells stimulated with autologous PHA-T cells and with autologous non-T cells displays circadian variations. The highest proliferation occurs with cells isolated from blood drawn at 8 a.m. in mixed lymphocyte reactions (MLR) with autologous PHA-T cells and from blood drawn at 8 p.m. in MLR with autologous non-T cells. The circadian variations of autologous MLRs appear to reflect changes in the proliferative response of T cells. In autologous MLRs with non-T cells as stimulators the extent of proliferation was inversely correlated with the level of endogenous cortisol. The circadian variations of autologous MLRs do not reflect non-specific changes in the proliferative and stimulatory properties of T and non-T cells, since circadian variations were not observed in the proliferative response of T cells to mitogens and in allogeneic MLRs. Circadian variations of autologous MLRs must be taken into account when analyzing abnormalities of these reactions in pathological conditions.
authors.forename: F
authors.initials: F
authors.lastname: Indiveri
authors.forename: I
authors.initials: I
authors.lastname: Pierri
authors.forename: S
authors.initials: S
authors.lastname: Rogna
authors.forename: A
authors.initials: A
authors.lastname: Poggi
authors.forename: P
authors.initials: P
authors.lastname: Montaldo
authors.forename: R
authors.initials: R
authors.lastname: Romano
authors.forename: A
authors.initials: A
authors.lastname: Pende
authors.forename: A
authors.initials: A
authors.lastname: Morgano
authors.forename: A
authors.initials: A
authors.lastname: Barabino
authors.forename: S
authors.initials: S
authors.lastname: Ferrone
mesh_terms: D002940:Circadian Rhythm; D006801:Humans; D006854:Hydrocortisone / Q000097:blood*; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D008214:Lymphocytes / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D006854:Hydrocortisone