Cultivation of mouse macrophages in vitro on different protein substrates.
Macrophages were isolated from the peritoneal cavity of mice and cultured in vitro under serum-free conditions on glass coverslips or glass coverslips coated with either collagen, fibronectin or fibrin. The recovery of greater than 95% pure populations of macrophages was highest on fibronectin and fibrin coats when compared to controls on glass coverslips. The cultivation of macrophages on fibronectin and fibrin coats induced a large degree of cell spreading, normally regarded as a parameter of stimulation in cultured macrophages. However, the ability of macrophages to lyse tumour cells and to release lysosomal enzymes was not found to differ to any considerable extent in cells cultured on the different substrates. Culturing macrophages on protein substrates may be used to study macrophage function in vitro, particularly under serum-free conditions.
Kolset
S O
SO
Department of Tumour Biology, University of Tromsø, Norway.
Bøgwald
J
J
D000818:Animals; D002448:Cell Adhesion; D002478:Cells, Cultured; D046508:Culture Techniques / Q000379:methods; D005966:Glucuronidase / Q000032:analysis; D008247:Lysosomes / Q000201:enzymology; D008264:Macrophages / Q000166:cytology* / Q000502:physiology / Q000648:ultrastructure; D051379:Mice; D008815:Mice, Inbred Strains; D008855:Microscopy, Electron, Scanning; D010587:Phagocytosis; D011506:Proteins
D011506:Proteins; D005966:Glucuronidase