A pentameric form of human serum amyloid P component. Crystallization, X-ray diffraction and neutron scattering studies.
Human serum amyloid P component crystallizes from sodium acetate buffer (pH 5.5) in the presence of calcium and polyethylene glycol 6000, at 4 degrees C. The space group is P2(1) and the cell parameters are a = 69.0 A (1 A = 0.1 nm), b = 99.3 A, c = 96.8 A, beta = 96.1. Density considerations supported by neutron scattering and gel filtration experiments indicate that the species crystallized is pentameric. The orientation of the pentamer 5-fold axis is determined and a crystal packing for the discs is proposed.
Wood
S P
SP
Department of Crystallography, Birkbeck College, London, U.K.
Oliva
G
G
O'Hara
B P
BP
White
H E
HE
Blundell
T L
TL
Perkins
S J
SJ
Sardharwalla
I
I
Pepys
M B
MB
D003460:Crystallization; D006801:Humans; D046911:Macromolecular Substances; D009502:Neutrons; D012542:Scattering, Radiation; D000683:Serum Amyloid P-Component; D014961:X-Ray Diffraction
D046911:Macromolecular Substances; D000683:Serum Amyloid P-Component