A monoclonal antibody to a human mast cell/myeloid leukaemia-specific antigen binds to normal haemopoietic progenitor cells and inhibits colony formation in vitro.
An antigen identified by murine monoclonal antibody YB5.B8 has previously been detected only on acute non-lymphoblastic leukaemia (ANLL) cells and tissue mast cells. We now report that the YB5.B8 antigen is present on a minor population (up to 3%) of normal bone marrow mononuclear cells which overlaps the set of progenitor cells capable of forming haemopoietic colonies in vitro. The results indicate that the antigen is a normal haemopoietic progenitor cell marker which is selectively retained on mast cells during maturation, and that leukaemias which express the antigen are not necessarily committed to the mast cell lineage. Furthermore, the antibody was capable of partially inhibiting the formation of haemopoietic colonies in vitro, indicating an important functional role for the antigen. This is consistent with the observation, reported in the accompanying paper, that expression of the YB5.B8 antigen is strongly correlated with poor response to therapy in patients with ANLL.
Cambareri
A C
AC
Department of Microbiology and Immunology, University of Adelaide, South Australia.
Ashman
L K
LK
Cole
S R
SR
Lyons
A B
AB
D000911:Antibodies, Monoclonal / Q000502:physiology*; D000912:Antibodies, Neoplasm / Q000502:physiology; D000951:Antigens, Neoplasm / Q000276:immunology*; D001666:Binding Sites, Antibody; D001853:Bone Marrow / Q000378:metabolism; D002455:Cell Division; D002469:Cell Separation; D003114:Colony-Forming Units Assay; D005434:Flow Cytometry; D006131:Growth Inhibitors / Q000502:physiology*; D006412:Hematopoietic Stem Cells / Q000378:metabolism* / Q000502:physiology; D006801:Humans; D007946:Leukemia, Mast-Cell / Q000276:immunology; D007951:Leukemia, Myeloid / Q000276:immunology; D012397:Rosette Formation
D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm; D006131:Growth Inhibitors