Proliferation and cell loss of human leukemic cell subpopulations in liquid culture.
A kinetic study was performed on leukemic blasts from patients with acute myeloid leukemia, separated into 2 subpopulations by a specific density gradient. The growth curve and the [3H]-thymidine uptake were simultaneously analyzed. While cumulative nucleotide uptake fitted with the growth kinetics in the low-density fraction, such a concordance was not found in the high-density subpopulation. That indicated the occurrence of simultaneous growth and loss in the high density fraction, which could not be evaluated by a simple numerical determination.
Bernabei
P A
PA
Div. Ematologia, Università degli Studi, Policlinico di Careggi, Firenze (Italy).
Agostino
F C
FC
Bezzini
R
R
Saccardi
R
R
Gattei
V
V
Santini
V
V
Casini
M
M
Rossi Ferrini
P
P
D002455:Cell Division; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000473:pathology*; D014407:Tumor Cells, Cultured / Q000473:pathology*