title: Preparation and characterization of monoclonal antibodies to cephalexin-synthesizing enzyme from Acetobacter turbidans.
abstract: Eleven monoclonal antibodies against the cephalexin-synthesizing enzyme have been constructed and primarily characterized. These antibodies are all IgG1 type, with medium affinity, and with no enzyme-inhibition effect. They will be utilized as immunoadsorbents to purify their corresponding antigen, the enzyme, in one step.
authors.affiliation: University of California, Davis 95616.
authors.forename: C Y
authors.initials: CY
authors.lastname: Yang
authors.forename: Y W
authors.initials: YW
authors.lastname: Ryu
authors.forename: D D
authors.initials: DD
authors.lastname: Ryu
mesh_terms: D000150:Acinetobacter / Q000201:enzymology*; D000217:Acyltransferases / Q000276:immunology* / Q000302:isolation & purification; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000915:Antibody Affinity; D006825:Hybridomas / Q000276:immunology; D007074:Immunoglobulin G / Q000096:biosynthesis; D051379:Mice
chemical_list: D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D000217:Acyltransferases; C019862:cephalosporin synthesizing enzyme