title: Genetic and biological characterization of a T suppressor cell induced by anti-idiotypic antibody.
abstract: The cellular and molecular characteristics of anti-idiotype-induced suppression have been investigated. We have shown that i.v. immunization of A/J or C.AL-20 mice with rabbit antibodies against the major cross-reactive idiotype on A/J anti-ABA antibodies induces splenic suppressor T cells (Ts) able to suppress T cell-mediated cytolytic and delayed-type hypersensitivity responses to ABA. In these studies, we compare the T suppressor activity manifested by anti-Id-induced suppressor cells with that described previously after conventional antigen priming. Results indicate that i.v. injection of anti-idiotypic antibodies primes for efferent level Ts; in contrast, i.v. administration of ABA-coupled cells induces afferent level suppressor cells. Soluble cell lysates, containing suppressor factor(s) derived from these anti-idiotype-induced Ts, can also mediate suppression of T cell immune responses in an efferent manner. Factor-mediated suppression is MHC-unrestricted and is also observed in mice pretreated with cyclophosphamide, suggesting that this activity is analogous to third-order suppression. Furthermore, this factor suppresses the T cell-mediated DTH and CTL responses in an antigen-nonspecific but Igh-restricted manner. These latter results suggest that the cellular elements conferring antigen specificity and Igh restriction are separate. The implications of these findings to the relationship between idiotypic elements, antigen-binding structures, and Igh restriction elements on immunoregulatory T cells are discussed.
authors.forename: J G
authors.initials: JG
authors.lastname: Monroe
authors.forename: M
authors.initials: M
authors.lastname: Gurish
authors.forename: J
authors.initials: J
authors.lastname: Dambrauskas
authors.forename: M
authors.initials: M
authors.lastname: Slaoui
authors.forename: A
authors.initials: A
authors.lastname: Lowy
authors.forename: M I
authors.initials: MI
authors.lastname: Greene
mesh_terms: D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D003520:Cyclophosphamide / Q000494:pharmacology; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D008040:Genetic Linkage; D006968:Hypersensitivity, Delayed / Q000276:immunology; D007111:Immunity, Cellular / Q000187:drug effects; D007143:Immunoglobulin Heavy Chains / Q000276:immunology*; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D008222:Lymphokines / Q000276:immunology; D008285:Major Histocompatibility Complex; D051379:Mice; D008815:Mice, Inbred Strains; D013601:T-Lymphocytes / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D010132:p-Azobenzenearsonate / Q000276:immunology
chemical_list: D000888:Antibodies, Anti-Idiotypic; D007143:Immunoglobulin Heavy Chains; D007130:Immunoglobulin Idiotypes; D008222:Lymphokines; D010132:p-Azobenzenearsonate; D003520:Cyclophosphamide