title: Structure and expression of an actin gene of Physarum polycephalum.
abstract: Physarum polycephalum (strain M3CVIII) contains four unlinked actin gene loci, each with two alleles (ardA1, ardA2, ardB1, ardB2, ardC1, ardC2, ardD1 and ardD2). The 4800 base HindIII fragment of the ardC2 allele was previously isolated as a recombinant phage lambda. We now report the structure of the actin gene sequences (C-actin gene). The gene, which contains four intervening sequences, codes for the principal actin isotype of plasmodia and it is expressed in both the haploid myxamoebal and diploid plasmodial phases of the life cycle. The C-actin isotype is closely related to actins of Dictyostelium, Acanthamoebae, Drosophila, sea urchin and mammalian cytoplasmic actin, and more distantly related to actins of yeast, Entamoebae and Tetrahymena. The ardC1 and ardC2 alleles differ by a 700(+/- 100) base-pair insertion/deletion in the vicinity of the 3' end of the transcribed region of the gene.
authors.affiliation: Laboratory of Developmental Biochemistry, National Institute for Medical Research, Mill Hill, London, U.K.
authors.forename: J A
authors.initials: JA
authors.lastname: Gonzalez-y-Merchand
authors.forename: R A
authors.initials: RA
authors.lastname: Cox
mesh_terms: D000199:Actins / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D010804:Physarum / Q000235:genetics*
chemical_list: D000199:Actins