Synthetic atrial natriuretic factor induces release (possibly receptor-mediated) of vasopressin from rat posterior pituitary.
The synthetic fragment (Arg 101-Tyr 126) of atrial natriuretic factor (ANF) induces release of arginine vasopressin from the isolated posterior lobe of the rat hypophysis in vitro. At a physiological concentration (3 X 10(-10)M) ANF was three times more effective than 61 mM KCL. In vitro binding studies with 125I-ANF revealed the presence of high affinity receptor sites displaying a pK = 9.9, a Kd = 0.14 nM, a Bmax = 20 fmol/posterior lobe and and IC50 = 200 pM. These results suggest that arginine vasopressin release by synthetic atrial natriuretic factor may be receptor mediated.
Januszewicz
P
P
Gutkowska
J
J
De Lean
A
A
Thibault
G
G
Garcia
R
R
Genest
J
J
Cantin
M
M
D000818:Animals; D001127:Arginine Vasopressin / Q000378:metabolism*; D009320:Atrial Natriuretic Factor; D004305:Dose-Response Relationship, Drug; D005260:Female; D009124:Muscle Proteins / Q000494:pharmacology*; D010904:Pituitary Gland, Posterior / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D009124:Muscle Proteins; C472610:atrial natriuretic peptide, rat; D001127:Arginine Vasopressin; D009320:Atrial Natriuretic Factor