title: Complete assignment of the 1H nuclear magnetic resonance spectrum of French bean plastocyanin. Application of an integrated approach to spin system identification in proteins.
abstract: The identification of the spin systems that comprise the 1H nuclear magnetic resonance spectrum of French bean Cu(I) plastocyanin (Mr 10,600) has been made using an approach that integrates a wide range of two-dimensional nuclear magnetic resonance experiments. A very large percentage of these assignments has been obtained in spectra acquired from 1H2O solution using a backbone amide-based strategy. The spin systems of 91 of the 99 residues have been assigned to the appropriate amino acid, thereby providing an ample basis for obtaining sequence-specific assignments, as described in the accompanying paper.
authors.affiliation: Department of Molecular Biology, Research Institute of Scripps Clinic, La Jolla, CA 92037.
authors.forename: W J
authors.initials: WJ
authors.lastname: Chazin
authors.forename: M
authors.initials: M
authors.lastname: Rance
authors.forename: P E
authors.initials: PE
authors.lastname: Wright
mesh_terms: D000595:Amino Acid Sequence; D007887:Fabaceae / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D010940:Plant Proteins; D010946:Plants, Medicinal; D010970:Plastocyanin
chemical_list: D010940:Plant Proteins; D010970:Plastocyanin