title: [Cultivation of the producer of alpha-amylase Bacillus subtilis under batch and continuous conditions].
abstract: The growth of Bacillus subtilis FU-79 and its production of alpha-amylase (EC 3.2.1.1) were studied under the conditions of batch and continuous cultivation in a semisynthetic medium. The enzyme activity fell down abruptly upon a pulse addition of either glucose or yeast extract to the chemostat culture, and remained at a low level for the following ten generations. Apparently, a double limitation of the culture growth (viz., with residual glucose and with yeast extract components) is required for the activity of alpha-amylase to be high.
authors.forename: S A
authors.initials: SA
authors.lastname: Lirova
authors.forename: L M
authors.initials: LM
authors.lastname: Ermakova
authors.forename: I L
authors.initials: IL
authors.lastname: Rabotnova
authors.forename: M P
authors.initials: MP
authors.lastname: Khovrychev
mesh_terms: D001412:Bacillus subtilis / Q000201:enzymology / Q000254:growth & development*; D004794:Enzyme Repression; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D000516:alpha-Amylases / Q000096:biosynthesis*
chemical_list: D000516:alpha-Amylases
vernacular_title: Kul'tivirovanie produtsenta alpha-amilazy Bacillus subtilis v periodicheskikh i nepreryvnykh usloviiakh.