Concomitant production of different lymphokines in activated T cells.
The intracellular accumulation of five different lymphokines in individual cells could be identified by lymphokine-specific antibodies and an indirect immunofluorescence technique with UV microscopy. When peripheral blood mononuclear cells from one healthy donor were activated in vitro by the T cell mitogen anti-CD3 monoclonal antibody, the maximal number of cells producing interleukin (IL)2, IL 6 or tumor necrosis factor (TNF)-alpha occurred 6 h after initiation of the cultures, while peak numbers of interferon-gamma, TNF-beta and a second wave of TNF-alpha-synthesizing cells were noted approximately 20 h later. By performing two-color immunofluorescence studies we observed a variegated production pattern with cells making no, one or several lymphokines simultaneously. All five cytokines accumulated in the Golgi organelle resulting in a very characteristic morphology of the staining with or without additional cytoplasmic immunofluorescence.
Andersson
U
U
Department of Immunology, Stockholm University, Sweden.
Sander
B
B
Andersson
J
J
Möller
G
G
D000911:Antibodies, Monoclonal / Q000276:immunology; D002478:Cells, Cultured; D005455:Fluorescent Antibody Technique; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis; D007376:Interleukin-2 / Q000096:biosynthesis; D015850:Interleukin-6; D007378:Interleukins / Q000096:biosynthesis; D008213:Lymphocyte Activation; D008222:Lymphokines / Q000096:biosynthesis*; D013601:T-Lymphocytes / Q000502:physiology*; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D000911:Antibodies, Monoclonal; D007376:Interleukin-2; D015850:Interleukin-6; D007378:Interleukins; D008222:Lymphokines; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma