Lymphokine production by human peripheral blood lymphocytes: analysis by in situ hybridization.
Lymphokine production in human peripheral blood mononuclear cells (PBMNCs) was investigated by in situ hybridization with 35S-labelled RNA probes coding for interleukin 2 (IL-2), gamma-interferon (gamma-IFN) and granulocyte/macrophage colony stimulating factor (GM-CSF). The proportion of PBMNCs expressing lymphokine mRNA varied according to the stimuli used and followed similar patterns to those previously observed by Northern blot analysis. Only a relatively low proportion of PBMNCs expressed lymphokine mRNA after stimulation for a given time, and this seems not to be due to the heterogeneity of T cells.
Qiu
G
G
Institute of Clinical Immunology, Inselspital, University of Bern, Switzerland.
Gauchat
J F
JF
Wirthmüller
U
U
De Weck
A L
AL
Stadler
B M
BM
D003115:Colony-Stimulating Factors / Q000096:biosynthesis / Q000235:genetics; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D006133:Growth Substances / Q000096:biosynthesis / Q000235:genetics; D006801:Humans; D066298:In Vitro Techniques; D007371:Interferon-gamma / Q000096:biosynthesis / Q000235:genetics; D007376:Interleukin-2 / Q000096:biosynthesis / Q000235:genetics; D008214:Lymphocytes / Q000276:immunology*; D008222:Lymphokines / Q000096:biosynthesis* / Q000235:genetics; D009693:Nucleic Acid Hybridization; D015347:RNA Probes; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
D003115:Colony-Stimulating Factors; D006133:Growth Substances; D007376:Interleukin-2; D008222:Lymphokines; D015347:RNA Probes; D012333:RNA, Messenger; D007371:Interferon-gamma; D016178:Granulocyte-Macrophage Colony-Stimulating Factor