Electrophoretic methods for screening for antibiotics in animal feeds.
A new system for screening antibiotics in animal feeds involves washing antibiotic-containing animal feeds with a series of organic solvents to eliminate interferences and partially separate the antibiotics. Antibiotics are then determined by using paper electrophoresis and thin-layer bioautography. This approach enables the separation and qualitative identification of 17 antibiotics currently used in animal feeds and provides the ability to detect low-level cross-contamination.
Salvatore
M J
MJ
Rutgers State University of New Jersey, Department of Biochemistry and Microbiology, New Brunswick 08903.
Katz
S E
SE
D000821:Animal Feed / Q000032:analysis*; D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000494:pharmacology; D001412:Bacillus subtilis / Q000187:drug effects; D001681:Biological Assay; D002021:Buffers; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel
D000900:Anti-Bacterial Agents; D002021:Buffers; D003470:Culture Media