Altered cytosol/membrane enzyme redistribution on interleukin-3 activation of protein kinase C.
Interleukin-3 (IL-3) is a member of a family of growth and differentiation peptides, collectively referred to as colony-stimulating factors, which regulate haematopoiesis. IL-3 has been highly purified from medium conditioned by WEHI-3B cells, and recently the molecular cloning of complementary DNA for murine IL-3 has been reported. IL-3 seems to stimulate a wide range of colony-forming cells derived from murine bone marrow and has consequently been studied under a variety of names, including burst-promoting activity, mast cell growth factor, P-cell stimulating factor and multi-colony-stimulating factor. Here we present evidence that IL-3-receptor interaction stimulates the rapid and transient redistribution of protein kinase C (PK-C) from cytosol to plasma membrane in FDC-P1 cells. Phorbol myristate acetate (PMA) is shown to have a similar effect in these IL-3-dependent FDC-P1 cells. Our data suggest that IL-3 and phorbol esters share a common feature of transmembrane signalling crucial for growth and differentiation.
Farrar
W L
WL
Thomas
T P
TP
Anderson
W B
WB
D000818:Animals; D002451:Cell Compartmentation / Q000187:drug effects; D002462:Cell Membrane / Q000201:enzymology*; D002478:Cells, Cultured; D003600:Cytosol / Q000201:enzymology; D004247:DNA / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D007377:Interleukin-3; D007700:Kinetics; D008222:Lymphokines / Q000494:pharmacology*; D051379:Mice; D011493:Protein Kinase C; D011494:Protein Kinases / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D007377:Interleukin-3; D008222:Lymphokines; D004247:DNA; D011494:Protein Kinases; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate