Cloning and expression of two Streptococcus mutans glucosyltransferases in Escherichia coli K-12.
Chromosomal DNA from Streptococcus mutans strain MFe28 (serotype h) was cloned in the bacteriophage vector lambda L47.1. Two classes of recombinants were found which expressed glucosyltransferase activity in phage plaques: (i) gtfS, which expressed a glucosyltransferase synthesizing a water-soluble, dextranase-sensitive glucan, and (ii) gtfI, which expressed a primer-dependent glucosyltransferase synthesizing an insoluble glucan.
Gilpin
M L
ML
Russell
R R
RR
Morrissey
P
P
D010582:Bacteriophage lambda / Q000235:genetics; D003001:Cloning, Molecular; D004274:DNA, Recombinant / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005796:Genes; D005798:Genes, Bacterial; D005964:Glucosyltransferases / Q000235:genetics*; D008970:Molecular Weight; D013295:Streptococcus mutans / Q000201:enzymology / Q000235:genetics*
D004274:DNA, Recombinant; D005964:Glucosyltransferases