title: Chloroacetaldehyde reacts with Z-DNA.
abstract: We show that chloroacetaldehyde, a chemical compound known to be reactive with unpaired adenine and cytosine residues, reacts with adenine residues (syn conformation) but not with cytosine residues (anti conformation) within Z-DNA. These modified residues are sensitive to cleavage by piperidine, which allows mapping at the single nucleotide level.
authors.affiliation: Centre de Biophysique Moléculaire, Orléans, France.
authors.forename: N
authors.initials: N
authors.lastname: Vogt
authors.forename: L
authors.initials: L
authors.lastname: Marrot
authors.forename: N
authors.initials: N
authors.lastname: Rousseau
authors.forename: B
authors.initials: B
authors.lastname: Malfoy
authors.forename: M
authors.initials: M
authors.lastname: Leng
mesh_terms: D000079:Acetaldehyde / Q000031:analogs & derivatives* / Q000378:metabolism; D001483:Base Sequence; D004247:DNA / Q000187:drug effects / Q000378:metabolism*; D009710:Nucleotide Mapping; D010880:Piperidines / Q000494:pharmacology
chemical_list: D010880:Piperidines; C032727:piperidine; D004247:DNA; C004656:chloroacetaldehyde; D000079:Acetaldehyde