title: Cysteine proteinase inhibitors in inflamed human gingiva.
abstract: In the present work we demonstrate the presence of cysteine proteinase inhibitors of all three classes: kininogens, stefin A, and cystatin C, in inflamed human gingiva. Using cystatin C, in inflamed human gingiva. Using immunochemical methods we found that stefin A is the major inhibitor of cysteine proteinases, followed by kininogen and cystatin C. The values for stefin A and cystatin C ranged from 7.0--400 micrograms/g and 1.5--6.1 micrograms/g tissue, respectively, as determined by enzyme-linked immunosorbent assay in inflamed gingival homogenates from patients with different degrees of periodontal disease.
authors.affiliation: Department of Biochemistry, J. Stefan Institute, E. Kardelj University, Ljubljana, Yugoslavia.
authors.forename: J
authors.initials: J
authors.lastname: Babnik
authors.forename: V
authors.initials: V
authors.lastname: Curin
authors.forename: T
authors.initials: T
authors.lastname: Lah
authors.forename: V
authors.initials: V
authors.lastname: Turk
authors.forename: U
authors.initials: U
authors.lastname: Skaleric
mesh_terms: D000917:Antibody Formation; D002846:Chromatography, Affinity; D055313:Cystatin B; D015891:Cystatins; D015853:Cysteine Proteinase Inhibitors; D004797:Enzyme-Linked Immunosorbent Assay; D005881:Gingiva / Q000201:enzymology*; D005891:Gingivitis / Q000201:enzymology*; D006801:Humans; D007120:Immunochemistry; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D007525:Isoelectric Focusing; D011480:Protease Inhibitors / Q000276:immunology / Q000302:isolation & purification* / Q000494:pharmacology; D011506:Proteins / Q000302:isolation & purification
chemical_list: C524994:CSTB protein, human; D015891:Cystatins; D015853:Cysteine Proteinase Inhibitors; D011480:Protease Inhibitors; D011506:Proteins; D055313:Cystatin B