title: Phase-system switching as an on-line sample pretreatment in the bioanalysis of mitomycin C using supercritical fluid chromatography.
abstract: One of the problems of the application of supercritical fluid chromatography (SFC) in bioanalysis is the fact that many sample pretreatment procedures deliver the solutes of interest in a polar solvent, which upon injection will dramatically disturb the phase system characteristics of the SFC system. The phase-system switching approach, recently introduced for liquid chromatography-mass spectrometry, can be used to avoid this problem. Plasma samples containing the thermolabile and pH-sensitive cytostatic drug Mitomycin C (MMC) were injected onto a short precolumn. After washing and drying of the precolumn the compound of interest was desorbed using a supercritical fluid and analyzed by SFC. Up to 1 ml of plasma containing 20 ng of MMC has been analyzed in this way.
authors.affiliation: Division of Analytical Chemistry, Gorlaeus Laboratories, Leiden University, The Netherlands.
authors.forename: W M
authors.initials: WM
authors.lastname: Niessen
authors.forename: P J
authors.initials: PJ
authors.lastname: Bergers
authors.forename: U R
authors.initials: UR
authors.lastname: Tjaden
authors.forename: J
authors.initials: J
authors.lastname: van der Greef
mesh_terms: D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods; D006801:Humans; D016685:Mitomycin; D008937:Mitomycins / Q000097:blood*
chemical_list: D008937:Mitomycins; D016685:Mitomycin