Composition of phospholipid molecular species from mammary tumors.
The molecular phospholipid species of mammary tumors induced by 7,12-dimethylbenz[a]anthracene in rats that were fed diets containing 20 or 3% sunflower-seed oil and different levels of calcium were analyzed by high-pressure liquid chromatography. Twenty-seven molecular species of phospholipids were identified. Phosphatidylcholine was predominantly composed of palmitoyl-arachidonoyl (16:0-20:4) (17-21%), palmitoyl-oleoyl (16:0-18:1) (19-21%), stearoyl-arachidonoyl (18:0-20:4) (12-13%), and 1,2-dipalmitoyl (16:0-16:0) (10-14%) species. The major molecular species of phosphatidylethanolamine were 18:0-20:4 (37-39%) and 16:0-20:4 (10-11%). The composition of diacyl phosphatidylcholine and diacyl phosphatidylethanolamine molecular species from rat mammary tumors was not greatly affected by the different diets.
Ishinaga
M
M
Department of Biochemistry, University of Western Ontario, London, Canada.
Carroll
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D015127:9,10-Dimethyl-1,2-benzanthracene / Q000633:toxicity; D000818:Animals; D014408:Biomarkers, Tumor / Q000032:analysis*; D002136:Calcium, Dietary / Q000008:administration & dosage; D005260:Female; D008325:Mammary Neoplasms, Experimental / Q000032:analysis*; D010713:Phosphatidylcholines / Q000032:analysis*; D010714:Phosphatidylethanolamines / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains
D014408:Biomarkers, Tumor; D002136:Calcium, Dietary; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D015127:9,10-Dimethyl-1,2-benzanthracene