title: Functional identification of t-PA in crude and purified systems.
abstract: In the present study the activation of glu-plasminogen by fibrin-bound t-PA was determined by integrating spectrophotometric and computer analysis of the reaction. Our aim was to determine the parameters of the activation and to characterize the functional activity of t-PA. Our results indicate that the t-PA present in purified or crude sources such as plasma or culture supernatants, can be specifically identified using the methodology described in this report.
authors.affiliation: INSERM U. 143, Hôpital de Bicêtre, France.
authors.forename: E
authors.initials: E
authors.lastname: Anglés-Cano
authors.forename: C
authors.initials: C
authors.lastname: Masson
authors.forename: P
authors.initials: P
authors.lastname: Grailhe
authors.forename: P
authors.initials: P
authors.lastname: Gaussem
authors.forename: B
authors.initials: B
authors.lastname: Le Bonniec
mesh_terms: D005337:Fibrin; D005340:Fibrinogen / Q000302:isolation & purification; D006801:Humans; D007700:Kinetics; D010958:Plasminogen / Q000302:isolation & purification; D010959:Tissue Plasminogen Activator / Q000032:analysis*
chemical_list: D005337:Fibrin; D005340:Fibrinogen; D010958:Plasminogen; D010959:Tissue Plasminogen Activator