Radioautographic localization of 125I-atrial natriuretic factor (ANF) in rat tissues.
Rats were injected either with synthetic 125I-Arg 101-Tyr 126 atrial natriuretic factor (ANF) or with 125I-ANF together with an excess of cold Arg 101-Tyr 126 ANF. Binding sites in various tissues were accepted depending on two criteria: displacement of radioactivity by cold ANF and absence of localization of silver grains on putative target cells in the presence of cold ANF. Binding sites were localized on zona glomerulosa cells and on adrenergic and noradrenergic cells of adrenal medulla, on hepatocytes, on the base of mature epithelial cells of villi in the small intestine, on smooth muscle cells of the muscularis layer of the colon and on the base of epithelial cells of the ciliary bodies. In addition, binding sites were localized in the vasculature of kidney, adrenal cortex, lung and liver. Binding sites were particularly numerous on renal glomerular endothelial cells. These results indicate that ANF may have important hemodynamic effects in kidney, lung, liver and adrenal cortex, may regulate water and ion transport in small intestine and ciliary bodies and may have metabolic effects in the liver. The presence of binding sites on the zona glomerulosa is in agreement with the important inhibitory effect of the peptide on aldosterone secretion.
Bianchi
C
C
Gutkowska
J
J
Thibault
G
G
Garcia
R
R
Genest
J
J
Cantin
M
M
D000311:Adrenal Glands / Q000378:metabolism; D000818:Animals; D009320:Atrial Natriuretic Factor; D001345:Autoradiography; D003106:Colon / Q000378:metabolism; D005123:Eye / Q000378:metabolism; D007421:Intestine, Small / Q000378:metabolism; D007457:Iodine Radioisotopes; D007672:Kidney Cortex / Q000378:metabolism; D007679:Kidney Medulla / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D009124:Muscle Proteins / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism; D051381:Rats / Q000378:metabolism*; D011919:Rats, Inbred Strains; D014018:Tissue Distribution
D007457:Iodine Radioisotopes; D009124:Muscle Proteins; D009320:Atrial Natriuretic Factor