Aplastic anemia associated with in vitro inhibition of erythropoiesis by bone marrow-adherent cells.
A patient with aplastic anemia that evolved following pure red cell aplasia is described. Cultures of the patient's marrow cells revealed greatly reduced numbers of primitive (BFU-E) and relatively mature (CFU-E) erythroid progenitors, but normal numbers of multipotential (CFU-GEMM) precursors. The BFU-E/CFU-GEMM and CFU-E/BFU-E ratios in the patient's marrow cell cultures were also reduced. T cell- or antibody-mediated inhibition of in vitro erythropoiesis could not be demonstrated in this patient. However, the patient's marrow-adherent cells suppressed the growth of autologous and allogeneic BFU-E and CFU-E, without influencing the growth of CFU-GEMM. Medium conditioned by the patient's adherent cells failed to inhibit the growth of normal erythroid precursors. Our findings suggest a role for marrow-adherent cells in the pathogenesis of aplastic anemia in this patient.
Merchav
S
S
Department of Hematology, Faculty of Medicine, Technion-Israel Institute of Technology, Haifa.
Tatarsky
I
I
Sharon
R
R
D000328:Adult; D000741:Anemia, Aplastic / Q000209:etiology / Q000473:pathology*; D001853:Bone Marrow / Q000473:pathology*; D002450:Cell Communication; D002469:Cell Separation; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D004920:Erythropoiesis; D006801:Humans; D008297:Male; D009000:Monocytes / Q000473:pathology; D012010:Red-Cell Aplasia, Pure / Q000150:complications