Characterization of laminin-stimulated adherence and motility in tumor cells.
Laminin was shown to stimulate attachment, spreading and motility in a line of murine tumor cells. The stimulated cells began to attach and spread within 1 h and remained attached and spread throughout a 48-hour observation period. Pretreatment of the cells with laminin for 24 h beforehand did not interfere with their ability to bind laminin in a second treatment. Laminin-stimulated attachment and spreading occurred in the absence of extracellular Ca2+ or Mg2+, but not in the absence of both divalent cations. A number of cell function inhibitors blocked the adherence response, but were only partially effective even at high concentrations. These characteristics of laminin-stimulated adherence are different from those of the adherence response stimulated by peptide chemotactic factors and phorbol esters.
Fligiel
S E
SE
Rodriguez
A F
AF
Knibbs
R N
RN
McCoy
J P
JP
Varani
J
J
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D002630:Chemotactic Factors / Q000494:pharmacology; D007797:Laminin / Q000494:pharmacology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009374:Neoplasms, Experimental / Q000473:pathology*; D010703:Phorbol Esters / Q000494:pharmacology; D011453:Prostaglandins / Q000494:pharmacology; D011971:Receptors, Immunologic / Q000032:analysis; D017474:Receptors, Laminin
D002630:Chemotactic Factors; D007797:Laminin; D010703:Phorbol Esters; D011453:Prostaglandins; D011971:Receptors, Immunologic; D017474:Receptors, Laminin; D002118:Calcium