Effect of myasthenic patient sera on the number and distribution of acetylcholine receptors in muscle and nerve-muscle cultures from rat. Correlations with clinical state.
We studied the functional activities (FA) of sera obtained from 83 myasthenic patients on rat muscle cultures. Using the same sets of cultures, two parameters were evaluated after exposure to sera: residual fraction (RF) of acetylcholine receptors (AChR) coupled to 125I-labelled alpha-bungarotoxin (alpha Bgt) (81 sera) and the number of rhodamine labelled clusters (56 sera). Two types of culture were assayed: muscle alone and nerve-muscle cocultures (12 cases). In all combinations (fluorescence, radiolabelling, muscle alone and nerve-muscle cocultures), we found a significant correlation between FA and antibody (Ab) titre, and no correlation between FA and clinical severity: only sera with a high or intermediate Ab titre were effective, whatever the clinical severity of disease. With active sera, AChR loss was about 50% whereas the disappearance of AChR clusters was quite complete, which suggests AChR redistribution induced by MG sera.
Eymard
B
B
CNRS UA 1159, Centre Chirurgical Marie-Lannelongue, Le Plessis-Robinson, France.
de la Porte
S
S
Pannier
C
C
Berrih-Aknin
S
S
Morel
E
E
Fardeau
M
M
Bach
J F
JF
Koenig
J
J
D000818:Animals; D001323:Autoantibodies / Q000097:blood / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D008297:Male; D009046:Motor Neurons / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D009132:Muscles / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D009157:Myasthenia Gravis / Q000097:blood / Q000276:immunology*; D051381:Rats; D011950:Receptors, Cholinergic / Q000187:drug effects / Q000276:immunology*
D001323:Autoantibodies; D011950:Receptors, Cholinergic