Fusion transfer of dopamine DA1 receptors to Friend erythroleukemia cells.
Dopamine DA1 receptors were transferred from rat striatal membranes to Friend erythroleukemia cells (Fc) by membrane fusion. The Fc cells lack DA1 receptors but have a functional adenylate cyclase system. The striatal membranes bearing DA1 receptors were treated with N-ethylmaleimide (NEM) prior to fusion to inactivate their intrinsic adenylate cyclase activity. Fusion of the NEM-treated membranes and the Fc cells was induced by polyethylene glycol treatment to form a functional system de novo, which could be demonstrated by measuring the increase in cAMP production after addition of dopamine. This method provides a possibility to study the functional competence of receptors in neural tissue in more detail.
Olasmaa
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M
Terenius
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D000818:Animals; D002421:Caudate Nucleus; D003342:Corpus Striatum; D005622:Friend murine leukemia virus; D006801:Humans; D004915:Leukemia, Erythroblastic, Acute; D007942:Leukemia, Experimental; D008297:Male; D008561:Membrane Fusion; D051381:Rats; D011919:Rats, Inbred Strains; D011954:Receptors, Dopamine; D017447:Receptors, Dopamine D1
D011954:Receptors, Dopamine; D017447:Receptors, Dopamine D1