Influence of uptake1 and uptake2 on the relationship between diffusion and metabolism of noradrenaline in the perfused rabbit ear artery.
3H-metabolite effluxes from each surface of the perfused rabbit ear artery were measured during a 30-min period of application of 3H-(-)-noradrenaline (NA) to the intimal surface, the adventitial surface, and both surfaces simultaneously. The arteries were from reserpine-treated rabbits and were perfused in Ca-free medium containing prazosin to prevent the constrictor activity of NA. During intimal application, 3H-normetanephrine (NMN) was the principal metabolite. Effects of hydrocortisone, and of cocaine, indicated that (a) 3H-NMN was derived largely via uptake2, (b) 3H-3,4-dihydroxyphenylethylene glycol (DOPEG) was derived largely via uptake1, and (c) uptake2 limited access of the NA to sites of uptake1. During adventitial application, 3H-DOPEG was the principal metabolite. Effects of cocaine and hydrocortisone indicated that (a) 3H-DOPEG was derived largely via uptake1, (b) 3H-NMN was only partly derived via uptake2, and (c) uptake1 limited access of the NA to sites of uptake2. When 3H-NA entered both surfaces simultaneously, the effluxes of the deaminated catechols were identical to those prevailing during adventitial entry of the amine. In contrast, the effluxes of NMN corresponded more closely to the sum of those prevailing during entry via each surface separately. It is suggested that, in the perfused artery, regional differences in the diffusivity of NA between the adventitia and media are primarily responsible for the marked influence which the surface of entry exerts on exogenous NA metabolism.
Morris
R G
RG
Department of Clinical and Experimental Pharmacology, University of Adelaide, Australia.
Venning
M G
MG
de la Lande
I S
IS
D000818:Animals; D001158:Arteries / Q000378:metabolism*; D002199:Capillary Permeability / Q000187:drug effects; D003042:Cocaine / Q000494:pharmacology; D004058:Diffusion; D004423:Ear / Q000098:blood supply; D006854:Hydrocortisone / Q000494:pharmacology; D009638:Norepinephrine / Q000378:metabolism*; D010477:Perfusion; D010643:Phenoxybenzamine / Q000494:pharmacology; D011817:Rabbits
D010643:Phenoxybenzamine; D003042:Cocaine; D006854:Hydrocortisone; D009638:Norepinephrine