Analysis of cytoplasmic factors in developmental cleavage of mouse embryo.
One-cell embryos from certain mouse strains were found incapable of developing beyond the 2-cell stage in vitro (2-cell block), but a microinjection of EDTA effectively overcame this block. When 2-cell arrested embryos were fused with embryos that had developed to the late 2-cell stage in vivo, the fusants developed beyond the 2-cell stage. Microinjection of cytoplasm of in vivo 2-cell embryos into 1-cell embryos also obviated the 2-cell block. Analyses of 35S-labeled embryos by 2-dimensional polyacrylamide gel electrophoresis indicated changes in synthetic protein patterns possibly related to this block.
Suzuki
S
S
Department of Obstetrics and Gynecology, Keio University, School of Medicine, Tokyo, Japan.
Komatsu
S
S
Kitai
H
H
Endo
Y
Y
Iizuka
R
R
Fukasawa
T
T
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002459:Cell Fusion / Q000187:drug effects; D003593:Cytoplasm / Q000032:analysis* / Q000187:drug effects; D004492:Edetic Acid / Q000494:pharmacology; D005260:Female; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains / Q000196:embryology*
D004492:Edetic Acid