[The composition of immune sera against Neisseria meningitidis studied by high-performance liquid chromatography].
The comparative study of the composition of immune rabbit sera to N. meningitidis, as well as nonimmune sera, has been made by the methods of HPLC and radial immunodiffusion. The quantitative evaluation of the main serum proteins by the two methods has shown the coincidence of the results yielded by these methods. To study the total level of IgM and IgG in the sera under study, a simple and rapid HPLC technique is proposed. The study of the stability of sera during storage (at 4-6 degrees C) has revealed that immune sera show greater stability during storage under such conditions in comparison with sera obtained from nonimmune animals.
Filatova
T N
TN
Churakova
N I
NI
Titova
E V
EV
Filippov
Iu V
IuV
Vartanian
Iu P
IuP
D000818:Animals; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D004356:Drug Storage; D007106:Immune Sera / Q000032:analysis*; D005779:Immunodiffusion; D007136:Immunoglobulins / Q000032:analysis; D009345:Neisseria meningitidis / Q000276:immunology*; D011817:Rabbits; D012709:Serum Albumin / Q000032:analysis; D013696:Temperature; D013997:Time Factors
D007106:Immune Sera; D007136:Immunoglobulins; D012709:Serum Albumin
Izuchenie sostava immunnykh syvorotok protiv Neisseria meningitidis metodom vysokoéffektivnoĭ zhidkostnoĭ khromatografii.