title: An enzyme-linked immunosorbent assay for the serodiagnosis of Campylobacter pylori-associated gastritis.
abstract: An ELISA system, using an acid extractable cell surface C. pylori antigen, has been used to detect anti-C. pylori antibodies in 176 endoscoped patients. The antibody concentration was related to histological diagnosis and colonisation with Campylobacter-like organisms. The criteria for sero-positivity was determined from the median antibody concentration in a group of 368 non-endoscoped control patients. The specificity and sensitivity of the sero-diagnostic assay for histological gastritis in antral biopsies was 83.0% and 95.6% respectively. The positive (79.3%) and negative predictive values (94.3%) indicate that such a sero-diagnostic assay could be used to screen patients prior to endoscopy.
authors.affiliation: Experimental Pathology Laboratory, PHLS, Centre for Applied Microbiology and Research, Porton Down, Salisbury, U.K.
authors.forename: D G
authors.initials: DG
authors.lastname: Newell
authors.forename: B J
authors.initials: BJ
authors.lastname: Johnston
authors.forename: M H
authors.initials: MH
authors.lastname: Ali
authors.forename: P I
authors.initials: PI
authors.lastname: Reed
mesh_terms: D000328:Adult; D000907:Antibodies, Bacterial / Q000032:analysis; D002167:Campylobacter / Q000276:immunology; D002169:Campylobacter Infections / Q000150:complications / Q000175:diagnosis*; D004724:Endoscopy; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005069:Evaluation Studies as Topic; D005756:Gastritis / Q000209:etiology* / Q000276:immunology / Q000473:pathology; D006801:Humans; D008875:Middle Aged; D012698:Serologic Tests / Q000379:methods*
chemical_list: D000907:Antibodies, Bacterial