Ion-exchange chromatography of proteins on a polyethyleneimine-grafted hydrophilic polymer for high-performance liquid chromatography.
A new, macroporous, synthetic polymer, Hydrophase HP-PEI, having highly hydrophilic surface characteristics enhanced by evenly distributed polyethyleneimine groups on its surface, was evaluated as an high-performance ion-exchange chromatographic packing material for the separation of proteins and nucleotides. A steel column (100 mm x 7.8 mm I.D.) packed with this material produces more than 33,000 plates per m. This base polymer included large proteins, such as thyroglobulin and apoferritin, in a size-exclusion mode. With various proteins it gave high recovery, high resolution and high capacity.
Kitagawa
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Interaction Chemicals Inc., Mountain View, CA 94043.
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation*; D002852:Chromatography, Ion Exchange; D006454:Hemoglobins / Q000032:analysis; D009711:Nucleotides / Q000302:isolation & purification; D011094:Polyethyleneimine; D011095:Polyethylenes; D011485:Protein Binding; D011506:Proteins / Q000302:isolation & purification*; D013056:Spectrophotometry, Ultraviolet
D006454:Hemoglobins; D009711:Nucleotides; D011095:Polyethylenes; D011506:Proteins; D011094:Polyethyleneimine