Serine proteinase esterolytic activity as an assay of cytotoxic reactions.
A method for assessing cytotoxicity in cell-mediated cytolysis as well as in reactions of natural killing and antibody-dependent cellular cytotoxicity through assaying the total esterolytic activity of serine proteinases (TEASP) is both sensitive and specific. TEASP levels in all cytotoxic tests correlated with the percentage of lysis of 51Cr-labelled target cells. The method permits the differential assessment of endoproteases in both the effector cell and the target cell.
Grando
S A
SA
Unit of Immunodermatology, Kiev State Advanced Training Institute, U.S.S.R.
Drannik
G N
GN
Kostromin
A P
AP
Glukhenky
B T
BT
Boiko
Y Y
YY
Korostash
T A
TA
Demidov
S V
SV
Senyuk
O F
OF
D000328:Adult; D000818:Animals; D000920:Antibody-Dependent Cell Cytotoxicity; D002474:Cell-Free System; D003601:Cytotoxicity Tests, Immunologic / Q000379:methods; D003602:Cytotoxicity, Immunologic; D005260:Female; D006801:Humans; D007694:Killer Cells, Natural / Q000201:enzymology / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008875:Middle Aged; D012697:Serine Endopeptidases / Q000378:metabolism*
D012697:Serine Endopeptidases