Coccus-shaped Bacillus subtilis cells are inhibited at stage 0 of sporulation.
RodA and rodB mutations cause rod-shaped Bacillus subtilis cells to become coccus-shaped when the growth temperature is increased from 30 to 45 degrees C. At 30 degrees C four rod strains sporulated as well as the genetically closely related rod+ strains. In contrast, at 45 degrees C the sporulation frequencies of rod strains decreased approximately 10(2)- to 10(4)-fold, while those of rod+ strains remained either unchanged or decreased only slightly. Temperature shift experiments and ultrastructural data indicated that coccus-shaped cells were unable to form prespore septa and were, therefore, inhibited at stage 0 of sporulation.
Cheung
H Y
HY
Laboratory of Molecular Biology, National Institute of Neurological and Communicative Disorders and Stroke, Bethesda, MD 20892.
Vitković
L
L
D001412:Bacillus subtilis / Q000235:genetics / Q000502:physiology* / Q000648:ultrastructure; D003470:Culture Media; D008854:Microscopy, Electron; D009154:Mutation; D013171:Spores, Bacterial; D013696:Temperature
D003470:Culture Media