title: Flow cytometry crossmatching for kidney transplantation.
abstract: 1. Overall, positive FCXM reactions were associated with graft losses in regrafts but not in primary transplants. 2. In transplants involving older female or CVA donors, a positive FCXM appeared to be deleterious in both first transplants and regrafts. If the donor were a younger male and not a CVA victim, only FCXM positive regrafts had low graft survival rates. 3. We presented a 3-color technique allowing the differentiation of antibody reactions against T cells, B cells, and monocytes. Another method allows the simultaneous evaluation of both IgG and IgM reactions against T cells. Using these kinds of methods may make better identification of antibodies that may be harmful to a transplant allograft possible.
authors.affiliation: Department of Surgery, Medical College of Virginia, Richmond.
authors.forename: D J
authors.initials: DJ
authors.lastname: Cook
authors.forename: P I
authors.initials: PI
authors.lastname: Terasaki
authors.forename: Y
authors.initials: Y
authors.lastname: Iwaki
authors.forename: G
authors.initials: G
authors.lastname: Terashita
authors.forename: J
authors.initials: J
authors.lastname: Fujikawa
authors.forename: J
authors.initials: J
authors.lastname: Gera
authors.forename: A
authors.initials: A
authors.lastname: Takeda
authors.forename: G
authors.initials: G
authors.lastname: Danovitch
authors.forename: J T
authors.initials: JT
authors.lastname: Rosenthal
authors.forename: R
authors.initials: R
authors.lastname: Fine
mesh_terms: D000961:Antilymphocyte Serum; D001402:B-Lymphocytes / Q000276:immunology; D005260:Female; D005434:Flow Cytometry; D005456:Fluorescent Dyes; D006085:Graft Survival; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans; D007518:Isoantibodies / Q000032:analysis; D016030:Kidney Transplantation / Q000276:immunology*; D008297:Male; D009000:Monocytes / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D000961:Antilymphocyte Serum; D005456:Fluorescent Dyes; D007518:Isoantibodies