title: High-performance liquid chromatographic method for determination of ciprofloxacin in biological fluids.
abstract: A simple and precise high-performance liquid chromatographic procedure has been developed for the determination in biological fluids of ciprofloxacin, a new, with extended antibacterial spectrum, quinoline carboxylic acid. The work-up procedure involves a chemical extraction step followed by isocratic chromatography on a reversed-phase analytical column, with ultraviolet detection. The detection limit for blood levels is 10 ng/ml. The calibration curve is linear from this detection limit to 10 microgram/ml. The statistical analysis of the correlation made between this assay and an agar diffusion procedure during a pharmacokinetic study suggests the existence of one or more active metabolites which could be mainly excreted in the bile.
authors.forename: F
authors.initials: F
authors.lastname: Jehl
authors.forename: C
authors.initials: C
authors.lastname: Gallion
authors.forename: J
authors.initials: J
authors.lastname: Debs
authors.forename: J M
authors.initials: JM
authors.lastname: Brogard
authors.forename: H
authors.initials: H
authors.lastname: Monteil
authors.forename: R
authors.initials: R
authors.lastname: Minck
mesh_terms: D000890:Anti-Infective Agents / Q000032:analysis* / Q000097:blood / Q000652:urine; D001646:Bile / Q000032:analysis; D001826:Body Fluids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D002939:Ciprofloxacin; D006801:Humans; D011804:Quinolines / Q000032:analysis* / Q000097:blood / Q000652:urine
chemical_list: D000890:Anti-Infective Agents; D011804:Quinolines; D002939:Ciprofloxacin