Sister-chromatid exchanges and chromosomal aberrations in chick embryos after treatment with the fungicide maneb.
The genotoxic potential of a commercial formulation of the fungicide maneb (Maneb 80, containing 80% manganese ethylenebisdithiocarbamate as active ingredient) in chick embryos was evaluated, using as genetic end-points the frequency of sister-chromatid exchanges (SCE) and of chromosomal aberrations. Unincubated eggs were dipped in 0, 0.5, 1.5, 4.5, 13.5 or 27 g/l maneb aqueous solutions for 30 sec. Eggs were then incubated for 4 days. Maneb significantly increased SCE values at 13.5 g/l and at 27 g/l (means 1.33 and 1.74) over the control value (mean 0.87). The concentration of 27 g/l, which corresponds to 10.8 times the recommended maximum application level for use in the field, also resulted in a high mortality rate. No clastogenic effects following exposure to maneb were observed.
Arias
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Dipartimento di Biologia, Università di Milano, Italy.
D000818:Animals; D002642:Chick Embryo; D002869:Chromosome Aberrations; D008344:Maneb / Q000633:toxicity*; D009152:Mutagenicity Tests; D009153:Mutagens; D012016:Reference Values; D012854:Sister Chromatid Exchange / Q000187:drug effects*; D013859:Thiocarbamates / Q000633:toxicity*
D009153:Mutagens; D013859:Thiocarbamates; D008344:Maneb