Determination of phosphate ions with an enzyme sensor system.
An enzyme sensor system for the determination of phosphate ions was constructed using immobilized enzymes and an oxygen electrode. The principle of this method is based upon the nucleoside phosphorylase catalyzed reaction for which the presence of inorganic phosphorus is indispensable. One assay could be completed within 3 min. This enzyme sensor was able to withstand at least 70 assays. This system was applicable to simple, rapid and continuous determination of phosphate ions in food.
Watanabe
E
E
Department of Food Science and Technology, Tokyo University of Fisheries, Japan.
Endo
H
H
Toyama
K
K
D015374:Biosensing Techniques; D005504:Food Analysis / Q000379:methods*; D010100:Oxygen / Q000032:analysis; D010430:Pentosyltransferases; D010710:Phosphates / Q000032:analysis*; D010756:Phosphoric Acids / Q000032:analysis; D011683:Purine-Nucleoside Phosphorylase; D014969:Xanthine Oxidase
D010710:Phosphates; D010756:Phosphoric Acids; C030242:phosphoric acid; D014969:Xanthine Oxidase; D010430:Pentosyltransferases; D011683:Purine-Nucleoside Phosphorylase; D010100:Oxygen