The effect of 2'deoxyguanosine on human lymphocyte responses. II. 2'deoxyguanosine enhances PWM-induced B cell differentiation.
Addition of 40 microM 2'deoxyguanosine (2'd Guo) results in enhancement of human lymphocyte cultures stimulated with pokeweed mitogen, assessed by staining intracytoplasmic immunoglobulins. Furthermore, B cell responses were enhanced under experimental conditions favouring T cell suppression rather than help. Experiments with enriched B cell fractions indicate that 2'dGuo does not directly stimulate B cell responses. The enhancement by 2'dGuo was still observed in the presence of irradiated (non-proliferating) T cells. The mechanism of enhancement by 2'dGuo is discussed in terms of its effect on proliferation-independent suppressor T cells.
Hanglow
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Lydyard
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D001402:B-Lymphocytes / Q000166:cytology / Q000187:drug effects*; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D003849:Deoxyguanosine / Q000494:pharmacology*; D006801:Humans; D007694:Killer Cells, Natural / Q000187:drug effects; D011043:Pokeweed Mitogens / Q000494:pharmacology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology
D011043:Pokeweed Mitogens; D003849:Deoxyguanosine