Recovery of malondialdehyde in urine as a 2,4-dinitrophenylhydrazine derivative after exposure to chloroform or hydroquinone.
Malondialdehyde (MDA) excretion in urine as an index for toxicological effects of chloroform and hydroquinone was evaluated. In a first series of experiments three groups of rats were used: non-pretreated rats (group I), starved rats (group II) and starved plus phenobarbital pretreated rats (group III). Chloroform (0.15 or 0.30 ml/kg, p.o.) was given as a single dose. The MDA excretion was related to the pretreatment, and in group III to liver damage. In a second series of experiments control rats were administered hydroquinone (100 or 200 mg/kg, p.o.), which induced a dose-related MDA excretion. These data indicate that the MDA assay was a selective and accurate marker for toxicological effects induced by the tested compounds.
Ekström
T
T
Department of Toxicology, National Institute of Occupational Health, Solna, Sweden.
Warholm
M
M
Kronevi
T
T
Högberg
J
J
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D002725:Chloroform / Q000633:toxicity*; D006873:Hydroquinones / Q000633:toxicity*; D008099:Liver / Q000201:enzymology; D008314:Malonates / Q000652:urine*; D008315:Malondialdehyde / Q000652:urine*; D009955:Ornithine Decarboxylase / Q000378:metabolism; D010659:Phenylhydrazines / Q000652:urine*; D051381:Rats; D011919:Rats, Inbred Strains
D006873:Hydroquinones; D008314:Malonates; D010659:Phenylhydrazines; C004787:2,4-dinitrophenylhydrazine; D008315:Malondialdehyde; D002725:Chloroform; D000410:Alanine Transaminase; D009955:Ornithine Decarboxylase; C031927:hydroquinone