Phorbol ester-induced stimulation of prostaglandin biosynthesis in human amnion cells.
Both phorbol 12-myristate 13-acetate (PMA) and phorbol 12,13-dibutyrate (10(-8)-10(-6) M) induced concentration-dependent increases in prostaglandin E2 (PGE2) production by human amnion cells, with maximum stimulations of 10.8-fold and 5.9-fold, respectively. 4 alpha-Phorbol 12,13-didecanoate, an inactive phorbol ester analogue, had little or no effect on PGE2 production by amnion cells. PMA and phorbol 12,13-dibutyrate (10(-7) M) induced a maximal increase in the rate of PGE2 biosynthesis within 15 min of treatment. These results suggest that there is an active protein kinase C present in amnion cells that is linked to arachidonic acid release and/or metabolism.
Lytton
F D
FD
Department of Reproductive Medicine, University of California San Diego, La Jolla 92093-0802.
Mitchell
M D
MD
D000650:Amnion / Q000187:drug effects* / Q000378:metabolism; D015232:Dinoprostone; D006801:Humans; D015240:Phorbol 12,13-Dibutyrate; D010703:Phorbol Esters / Q000494:pharmacology*; D011458:Prostaglandins E / Q000096:biosynthesis*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D010703:Phorbol Esters; D011458:Prostaglandins E; C008876:phorbol-12,13-didecanoate; D015240:Phorbol 12,13-Dibutyrate; D015232:Dinoprostone; D013755:Tetradecanoylphorbol Acetate