title: Quantification of the enantiomers of ofloxacin in biological fluids by high-performance liquid chromatography.
abstract: Two methods for the determination of (+)- and (-)-ofloxacin in biological fluids by high-performance liquid chromatography are described. The first method is separation on a chiral stationary phase with bovine serum albumin immobilized on silica gel. The second is the coupling of ofloxacin to L-leucinamide via diphenylphosphinyl chloride activation. The diastereoisomeric derivatives are then separated on a common reversed-phase column. The second method revealed only slight differences in the pharmacokinetics of (+)- and (-)-ofloxacin in humans after an intravenous administration of racemic ofloxacin.
authors.affiliation: Hoechst AG, Frankfurt am Main, F.R.G.
authors.forename: K H
authors.initials: KH
authors.lastname: Lehr
authors.forename: P
authors.initials: P
authors.lastname: Damm
mesh_terms: D000890:Anti-Infective Agents / Q000032:analysis* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D006801:Humans; D007202:Indicators and Reagents; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D015242:Ofloxacin; D010078:Oxazines / Q000032:analysis* / Q000493:pharmacokinetics; D013237:Stereoisomerism
chemical_list: D000890:Anti-Infective Agents; D007202:Indicators and Reagents; D010078:Oxazines; D015242:Ofloxacin