Ultrastructural localization of phenoloxidases in cuticle and haemopoietic tissue of the blowfly Lucilia cuprina.
The ultrastructural localization of two types of biochemically characterized phenol oxidase activity is described in the larva of the sheep blowfly, Lucilia cuprina. Cuticular tyrosinase activity (enzyme A) is seen in epicuticular filaments and procuticle. Procuticle activity can be detected only after a presumed process of activation takes place in damaged cuticle. By using either the dopamine reaction or inducing melanization by hot-water treatment, tyrosinase is readily shown in haemopoietic tissue which, in L. cuprina, occurs subdermally as well as being associated with the dorsal vessel. The adaptation of the diaminobenzidine technique, used to stain laccase in electrophoretic gels, to ultrastructural cytochemistry has made it possible to demonstrate enzymic activity probably due to laccase (enzyme B). The laccase activity is present in the inner epicuticle of late wandering third instar larvae (about to pupariate) but is not present in the epicuticle of younger larvae.
Binnington
K C
KC
CSIRO, Division of Entomology, Canberra, ACT, Australia.
Barrett
F M
FM
D015100:3,3'-Diaminobenzidine; D000818:Animals; D004156:Catechol Oxidase / Q000032:analysis*; D004298:Dopamine; D004589:Electrophoresis, Disc; D004817:Epidermis / Q000201:enzymology / Q000648:ultrastructure; D006413:Hematopoietic System / Q000201:enzymology* / Q000648:ultrastructure; D007313:Insecta / Q000201:enzymology* / Q000648:ultrastructure; D007814:Larva / Q000201:enzymology / Q000648:ultrastructure; D008854:Microscopy, Electron; D014442:Monophenol Monooxygenase / Q000032:analysis*
D015100:3,3'-Diaminobenzidine; D004156:Catechol Oxidase; D014442:Monophenol Monooxygenase; D004298:Dopamine