title: The induction of glycogenolysis in the perfused liver by platelet activating factor is mediated by prostaglandin D2 from Kupffer cells.
abstract: Induction of glycogenolysis in the perfused liver by platelet activating factor (PAF) was blocked by the cyclooxygenase inhibitor indomethacin. 3H-labeled PAF was shown to interact in the perfused liver primarily with Kupffer cells. The addition of PAF to Kupffer cells resulted in a dose-dependent stimulation of prostaglandin D2 (PGD2) production, which was identified as the main eicosanoid formed after PAF stimulation of the Kupffer cells. PGD2 was able to induce a dose-dependent stimulation of glycogenolysis both in the perfused liver and in isolated parenchymal cells. The time-dependency of the PGD2 production and the glucose output by the perfused liver is consistent with a primary interaction of PAF with the Kupffer cells, followed by PGD2 formation, which subsequently stimulates glucose production in parenchymal cells.
authors.affiliation: Division of Biopharmaceutics, Sylvius Laboratories, University of Leiden, The Netherlands.
authors.forename: J
authors.initials: J
authors.lastname: Kuiper
authors.forename: Y B
authors.initials: YB
authors.lastname: De Rijke
authors.forename: F J
authors.initials: FJ
authors.lastname: Zijlstra
authors.forename: M P
authors.initials: MP
authors.lastname: Van Waas
authors.forename: T J
authors.initials: TJ
authors.lastname: Van Berkel
mesh_terms: D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism; D016861:Cyclooxygenase Inhibitors; D005947:Glucose / Q000378:metabolism; D006003:Glycogen / Q000378:metabolism*; D007213:Indomethacin / Q000494:pharmacology; D007728:Kupffer Cells / Q000378:metabolism*; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D010477:Perfusion; D010972:Platelet Activating Factor / Q000494:pharmacology*; D015230:Prostaglandin D2 / Q000096:biosynthesis* / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D001095:Arachidonic Acids; D016861:Cyclooxygenase Inhibitors; D010972:Platelet Activating Factor; D016718:Arachidonic Acid; D006003:Glycogen; D005947:Glucose; D015230:Prostaglandin D2; D007213:Indomethacin