A rapid purification method for calf thymus casein kinase II.
We report here the rapid purification to homogeneity of a cyclic nucleotide-independent protein kinase sensitive to 5'6-dichloro-1-beta-D-ribofuranozylbenzimidazole (DRB), identical to the previously described casein kinase II, from lyophilized calf thymus by chromatography on phosphocellulose and Mono-Q FPLC columns.
Zandomeni
R
R
Comparative Leukemia Studies Unit, University of Pennsylvania, Kennett Square 19348.
Zandomeni
M C
MC
Weinmann
R
R
D000818:Animals; D047388:Casein Kinases; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D004004:Dichlororibofuranosylbenzimidazole / Q000494:pharmacology; D008970:Molecular Weight; D011494:Protein Kinases / Q000302:isolation & purification* / Q000378:metabolism; D012995:Solubility; D013053:Spectrophotometry; D013950:Thymus Gland / Q000201:enzymology*
D004004:Dichlororibofuranosylbenzimidazole; D011494:Protein Kinases; D047388:Casein Kinases