[Ultrahistochemical demonstration of blood group B substances on human erythrocytes with a lectin from Salmo gairdneri RICH].
From the roe of Salmo gairdneri RICH., a lectin was isolated which agglutinates specifically human erythrocytes of blood group B. For cytochemical labelling of the blood group substance B on the surface of human erythrocytes, an indirect approach was chosen. By means of a polyclonal antibody from the rabbit against the B-specific fish lectin, electronmicroscopic presentation was performed with Protein A gold using a multistep method. For quantification gold labelling was partially followed by a silver technique.
Feuerstein
H
H
Institut für Anatomie, Friedrich-Schiller-Universität Jena, DDR.
Linss
W
W
Reuter
E
E
Zimmermann
N
N
D000017:ABO Blood-Group System; D000818:Animals; D000906:Antibodies; D004910:Erythrocyte Membrane / Q000276:immunology* / Q000648:ultrastructure; D006801:Humans; D007150:Immunohistochemistry; D037102:Lectins; D008854:Microscopy, Electron; D014337:Trout
D000017:ABO Blood-Group System; D000906:Antibodies; D037102:Lectins
Ultrahistochemischer Nachweis der Blutgruppensubstanz B menschlicher Erythrocyten mit einem Lectin aus Salmo gairdneri RICH.