title: Immunoelectron microscopic studies on spore coat proteins of Bacillus megaterium.
abstract: An immunochemical staining technique for the spore coat proteins of Bacillus megaterium ATCC 12872 was developed using colloidal gold as a second antibody. For reducing the non-specific immunogold binding and increasing the specific binding, the affinity-purified IgG was used as a first antibody. In sporulating cells at t10, gold particles were found not only in the spore coat but also in the mother cell cytoplasm, suggesting that some coat proteins were synthesized in the cytoplasm. Use of the specific affinity-purified antibody to 48K-protein demonstrated that this protein was one of the components of the outer coat.
authors.affiliation: Faculty of Pharmaceutical Sciences, Osaka University Suita.
authors.forename: M
authors.initials: M
authors.lastname: Imagawa
authors.forename: M
authors.initials: M
authors.lastname: Ohtsuka
authors.forename: Y
authors.initials: Y
authors.lastname: Nakatani
authors.forename: Y
authors.initials: Y
authors.lastname: Oku
authors.forename: H I
authors.initials: HI
authors.lastname: el-Belbasi
authors.forename: T
authors.initials: T
authors.lastname: Nishihara
authors.forename: M
authors.initials: M
authors.lastname: Kondo
mesh_terms: D001410:Bacillus megaterium / Q000032:analysis*; D008565:Membrane Proteins / Q000032:analysis*; D008854:Microscopy, Electron; D008970:Molecular Weight; D013171:Spores, Bacterial / Q000032:analysis* / Q000648:ultrastructure
chemical_list: D008565:Membrane Proteins