Extended storage of platelets in a new plastic container. II. In vivo response to infusion of platelets stored for 5 days.
A new blood container material (PL 1240 plastic) made of polyvinyl chloride containing a tri(2-ethylhexyl) trimellitate plasticizer was evaluated in three laboratories. When platelet concentrates (50-60 ml) were stored on a variety of agitators for 7 days at 22 +/- 2 degrees C, poststorage pH (mean +/- SD) ranged from 7.29 +/- 0.05 (6 rpm elliptical rotator) to 6.87 +/- 0.8 (70 cycles per minute flatbed agitator). The platelet counts ranged from 1.51 +/- 0.12 to 0.95 +/- 0.36 X 10(6) per microliter. Morphology scores and hypotonic shock response values of platelets stored 7 days in PL 1240 plastic containers were better than those noted following 3-day storage of control platelets in PL 146 plastic containers. The percent discharge of lactic dehydrogenase from platelets stored 7 days in PL 1240 plastic containers for 3 days (p less than 0.05). Mean platelet recoveries of 44 +/- 15 percent (n = 11; 111Indium) and 39 +/- 8 percent (n = 29; 51Chromium) were seen when autologous platelets were infused following 5-day storage in PL 1240 plastic bags. Platelet half-lives of 3.6 +/- 0.4 (n = 9) 4.1 +/- 0.4 (n = 20) days were reported in the two laboratories which used 51Cr labeling, while survival values of 7.0 +/- 1.0, 2.8 +/- 0.8, and 5.4 +/- 1.9 days were seen when data from the 111Indium studies (n = 11) were analyzed using linear, exponential, and multiple hit programs, respectively. Platelets stored for 5 days also were administered to 13 thrombocytopenic oncology patients.(ABSTRACT TRUNCATED AT 250 WORDS)
Snyder
E L
EL
Ezekowitz
M
M
Aster
R
R
Murphy
S
S
Ferri
P
P
Smith
E
E
Rzad
L
L
Davisson
W
W
Pope
C
C
Kakaiya
R
R
D001792:Blood Platelets / Q000502:physiology; D001793:Blood Preservation / Q000295:instrumentation*; D002463:Cell Membrane Permeability; D002470:Cell Survival; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009996:Osmotic Fragility; D010968:Plasticizers; D017713:Platelet Transfusion; D011143:Polyvinyl Chloride; D013997:Time Factors; D001620:beta-Thromboglobulin / Q000378:metabolism
D010968:Plasticizers; D001620:beta-Thromboglobulin; D011143:Polyvinyl Chloride