In vitro study of Fc-receptor function in autoimmune diseases.
A simple test for studying in vitro Fc-receptor function of mononuclear phagocytes is described. Immune phagocytosis is analyzed as a dynamic phenomenon by using nearly pure suspensions of monocytes incubated for diverse times with autologous erythrocytes sensitized with highly purified IgG. In a series of normal volunteers and patients with vasculitis a strict correlation has been found between this in vitro assay and the measure of splenic clearance of IgG-coated red blood cells (RBC), the classical approach for studying in vivo macrophage Fc-receptor function by using sodium chromate 51Cr as tag. The use of this in vitro assay appears to be valuable mainly in cases requiring repeated measurements of Fc-receptor function for monitoring the course of disease or the effects of therapy.
Roccatello
D
D
Bensa
J C
JC
Coppo
R
R
Rollino
C
C
Diaz
M
M
Martina
G
G
Piccoli
G
G
D001327:Autoimmune Diseases / Q000276:immunology*; D002860:Chromium Radioisotopes; D004905:Erythrocyte Aging; D004912:Erythrocytes / Q000502:physiology; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism; D009000:Monocytes / Q000276:immunology / Q000378:metabolism*; D010587:Phagocytosis; D011961:Receptors, Fc / Q000502:physiology*; D017452:Receptors, IgG
D002860:Chromium Radioisotopes; D007074:Immunoglobulin G; D011961:Receptors, Fc; D017452:Receptors, IgG