The cloned cel (cellulose degradation) genes of Clostridium thermocellum and their products.
More than 10 cel genes of Clostridium thermocellum were cloned and expressed in Escherichia coli. Four of them, celA, celB, celC and celD, were studied in detail. The corresponding endoglucanases were purified from E. coli and characterized. The over-produced endoglucanase D, purified from cytoplasmic granules, was crystallized.
Béguin
P
P
Département des Biotechnologies, Institut Pasteur, Paris, France.
Millet
J
J
Aubert
J P
JP
D000595:Amino Acid Sequence; D001483:Base Sequence; D002480:Cellulase / Q000235:genetics; D002482:Cellulose / Q000378:metabolism*; D003013:Clostridium / Q000235:genetics* / Q000378:metabolism; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics
D012333:RNA, Messenger; D002482:Cellulose; D002480:Cellulase