Morphological changes and catalase activity in the hearts of CD 1 mice following acute starvation or single doses of doxorubicin, epirubicin or mitoxantrone.
The cardiac morphology of CD 1 mice undergoing two different schedules of acute (5 day) starvation and that of animals treated with a single dose (15 mg/kg i.p.) of doxorubicin, epirubicin or mitoxantrone were studied by light microscopy. Determinations of heart catalase were also carried out. Mice subjected to moderate starvation had a mean weight reduction of 18.7% and did not show heart morphological damage. A slight increase (38%) of heart catalase specific activity occurred in these animals. In animals subjected to severe starvation the weight loss was 32.2%. In this case considerable heart damage, in the form of myofibrillar loss, and a striking increase of catalase (158.5%) were seen. In the drug groups comparable weight reductions (about 15%) occurred 5 days after the treatment. Moderate heart lesions, represented by myolysis and especially by myocytic microvacuolation, were observed and appeared to be of similar degree in the 3 drug groups. Catalase specific activity increased by 119.9% in the doxorubicin animals, by 73% in the epirubicin mice and by 30.3% in the mitoxantrone ones. Light microscopy made it possible to distinguish between cardiac alterations induced by starvation and those specifically induced by antiblastics. Catalase may be helpful to indicate the existence of heart damage but it does not correlate well with the severity of the lesions by antiblastics. An additional cause of heart catalase elevation might be the free radical generation induced by the anthracyclines but not by mitoxantrone.
Crescimanno
M
M
Istituto di Farmacologia e Sezione Autonoma di Oncologia Clinica, Policlinico P. Giaccone, Palermo, Italy.
Flandina
C
C
Rausa
L
L
Sanguedolce
R
R
D'Alessandro
N
N
D000818:Animals; D002374:Catalase / Q000032:analysis*; D004317:Doxorubicin / Q000633:toxicity*; D015251:Epirubicin; D006321:Heart / Q000187:drug effects; D051379:Mice; D008942:Mitoxantrone / Q000633:toxicity*; D009206:Myocardium / Q000201:enzymology / Q000473:pathology*; D013217:Starvation / Q000201:enzymology / Q000473:pathology*
D015251:Epirubicin; D004317:Doxorubicin; D008942:Mitoxantrone; D002374:Catalase