Rapid and sensitive determination of zearalenone in cereals by high-performance liquid chromatography with fluorescence detection.
Zearalenone, an estrogenic mycotoxin of Fusarium species, in cereals can be extracted with acetonitrile-water (3:1), purified on a Florisil column, resolved by high-performance liquid chromatography (HPLC) with a Nucleosil 50-10 column using 90% water-saturated chloroform-cyclohexane-acetonitrile-ethanol (50:15:2:1) and quantitated by fluorescence measurement. This method is rapid, simple and reproducible, and detects zearalenone in wheat, barley, corn and other cereals with picogram sensitivity. A combination of this HPLC method with a gas-liquid chromatographic method for trichothecenes may be applied to the simultaneous detection of Fusarium mycotoxins (zearalenone, nivalenol and deoxynivalenol) in cereals.
Tanaka
T
T
Hasegawa
A
A
Matsuki
Y
Y
Lee
U S
US
Ueno
Y
Y
D002851:Chromatography, High Pressure Liquid; D002523:Edible Grain / Q000032:analysis*; D005516:Food Microbiology; D001467:Hordeum / Q000032:analysis; D012118:Resorcinols / Q000032:analysis*; D013050:Spectrometry, Fluorescence; D014255:Trichothecenes / Q000032:analysis; D014908:Triticum / Q000032:analysis; D003313:Zea mays / Q000032:analysis; D015025:Zearalenone / Q000032:analysis*
D012118:Resorcinols; D014255:Trichothecenes; D015025:Zearalenone; C038405:nivalenol; C007262:deoxynivalenol