title: Further characterization of SOS system induction in recBC mutants of Escherichia coli.
abstract: Expression of several SOS functions such as induction of lambda prophage, inhibition of cell division and induction of both umuC and recA genes after UV-irradiation, nalidixic acid or mitomycin C addition was studied in an RecBC- mutant. UV-irradiation and mitomycin C induced all SOS functions studied in the RecBC- cells but at a lower level and delayed with respect to the wild-type strain. On the contrary, nalidixic acid was unable to trigger any of these SOS functions. In the RecBC- mutant, adenine only had a stimulating effect on the amplification of RecA protein synthesis following UV-irradiation. Nevertheless, in the wild-type strain the stimulating effect occurred in all SOS functions studied following UV-irradiation as well as in the amplification of RecA protein synthesis by nalidixic acid but not in the other SOS functions triggered by this compound. Furthermore, adenine produced a decrease in the mitomycin C-mediated induction of all SOS functions studied in both RecBC- and wild-type strains.
authors.forename: J
authors.initials: J
authors.lastname: Barbé
authors.forename: J A
authors.initials: JA
authors.lastname: Vericat
authors.forename: J
authors.initials: J
authors.lastname: Cairó
authors.forename: R
authors.initials: R
authors.lastname: Guerrero
mesh_terms: D000225:Adenine / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000378:metabolism; D010582:Bacteriophage lambda / Q000187:drug effects; D004260:DNA Repair; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D005798:Genes, Bacterial; D009154:Mutation; D009268:Nalidixic Acid / Q000494:pharmacology; D014775:Virus Activation / Q000187:drug effects
chemical_list: D009268:Nalidixic Acid; D000255:Adenosine Triphosphate; D000225:Adenine