G-proteins and egg activation.
G-proteins are present in eggs, and experiments in which GTP-gamma-S, GDP-beta-S, cholera toxin and pertussis toxin have been injected into eggs have indicated the involvement of G-proteins in egg activation at fertilization and in oocyte maturation. Eggs into which serotonin or muscarinic acetylcholine receptors have been introduced by mRNA injection produce fertilization-like responses when exposed to serotonin or acetylcholine; since these neurotransmitter receptors act by way of G-proteins, this observation further supports the conclusion that a G-protein is involved in the fertilization process.
Jaffe
L A
LA
Department of Physiology, University of Connecticut Health Center, Farmington 06032.
Turner
P R
PR
Kline
D
D
Kado
R T
RT
Shilling
F
F
D000818:Animals; D005089:Exocytosis / Q000187:drug effects; D005260:Female; D005306:Fertilization / Q000187:drug effects*; D019204:GTP-Binding Proteins / Q000378:metabolism / Q000502:physiology*; D008297:Male; D009865:Oocytes / Q000187:drug effects / Q000502:physiology; D010063:Ovum / Q000378:metabolism / Q000502:physiology*; D012617:Sea Urchins; D013084:Sperm-Ovum Interactions / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects
D019204:GTP-Binding Proteins