A cortical granule-specific enzyme, B-1,3-glucanase, in sea urchin eggs.
The ultrastructural localization of B-1,3-glucanase in three species of sea urchin eggs was determined using a monospecific antibody in an electronmicroscopic immunogold procedure. In all three species, Lytechinus variegatus, Strongylocentrotus purpuratus, and Arbacia punctulata, B-1,3-glucanase was localized specifically to the cortical granules. No other organelle within the egg contained significant label. During the fertilization reaction, B-1,3-glucanase was released from cortical granules into the perivitelline space and became associated with the hyaline layer. No significant label was found in association with the fertilization envelope.
Wessel
G M
GM
Department of Zoology, Duke University, Durham, North Carolina.
Truschel
M R
MR
Chambers
S A
SA
McClay
D R
DR
D000818:Animals; D000911:Antibodies, Monoclonal; D003594:Cytoplasmic Granules / Q000201:enzymology* / Q000648:ultrastructure; D005260:Female; D043326:Glucan 1,3-beta-Glucosidase; D005959:Glucosidases / Q000032:analysis*; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D010063:Ovum / Q000201:enzymology* / Q000502:physiology / Q000648:ultrastructure; D012617:Sea Urchins; D013045:Species Specificity; D001617:beta-Glucosidase / Q000032:analysis*
D000911:Antibodies, Monoclonal; D005959:Glucosidases; D001617:beta-Glucosidase; D043326:Glucan 1,3-beta-Glucosidase