title: Amyloid in the horse: a report of nine cases.
abstract: Out of approximately 16,000 horses referred for clinical examination, nine had amyloidosis. Six of these horses had localised amyloid deposits in the wall of the nasal meatus and ventral turbinates associated with epistaxis. Horse 1 also developed malignant histiolymphocytic lymphosarcomas. The amyloid deposits were potassium permanganate-resistant and tryptophan-positive. Gel filtration of solubilised amyloid fibrils from Horse 1 revealed a major retarded fraction with an apparent molecular weight of 20 kD. This protein had an amino acid composition similar to human AL-amyloid proteins and horse immunoglobulin light chains. On Western blot a strong cross-reaction was observed between horse 1gG2a light chains and the Horse 1 amyloid. Horses 7 to 9 had suppurative verminous aneurysm, tuberculosis and an adrenal cortical adenoma, respectively, and had generalised amyloid deposits in liver and spleen. These amyloid deposits were found to be potassium permanganate-sensitive and positive for tryptophan. Gel filtration of solubilised amyloid fibrils from Horse 8 revealed a major retarded fraction (protein AA) with an apparent molecular weight of 10 kD. Immunoperoxidase-antiperoxidase staining showed the localised deposits to be negative or only weakly positive with antisera against bovine, hamster, dog and human protein AA and to be positive with anti-horse-one amyloid protein. The generalised deposits were found to be positive with the antisera against allogenic protein AA. The results of the potassium permanganate incubation, biochemistry, immunoblotting and immunochemistry, indicate that the localised amyloid of Horse 1 and most likely the amyloid of Horses 2 to 6, is of the AL-type. The generalised amyloid deposits were found to be of the AA type.
authors.affiliation: Department of Pathology, Veterinary Faculty, University of Utrecht, The Netherlands.
authors.forename: A C
authors.initials: AC
authors.lastname: van Andel
authors.forename: E
authors.initials: E
authors.lastname: Gruys
authors.forename: J
authors.initials: J
authors.lastname: Kroneman
authors.forename: J
authors.initials: J
authors.lastname: Veerkamp
mesh_terms: D000682:Amyloid / Q000145:classification; D000686:Amyloidosis / Q000145:classification / Q000453:epidemiology / Q000473:pathology / Q000662:veterinary*; D000818:Animals; D005260:Female; D006734:Horse Diseases / Q000453:epidemiology / Q000473:pathology*; D006736:Horses; D008297:Male
chemical_list: D000682:Amyloid