title: Effects of lentinan on cytotoxic functions of human lymphocytes.
abstract: The in vitro effects of lentinan on natural killer (NK), antibody-dependent cell-mediated cytotoxicity (ADCC), lectin-dependent cell-mediated cytotoxicity (LDCC) and mitogen-induced blast transformation were studied in patients with solid tumors and chronic lymphocytic leukemia (CLL). NK activity was measured against 51Cr-labelled K-562 targets, ADCC against antibody-coated chicken red cells. LDCC and natural cell-mediated cytotoxicity (NCMC) was assessed using 3H-thymidine prelabelled HEp-2 targets. Mitogen (PHA- and Con A-) induced blast transformation was measured by thymidine incorporation. Blastogenesis and LDCC was not influenced by lentinan. 1 microgram/ml lentinan increased NCMC of tumor-bearing subjects. The most prominent enhancement of NK and ADCC activity was seen in CLL patients, where a dose-related increase was seen (from 0.01 to 1 microgram/ml).
authors.affiliation: Second Department of Medicine, Semmelweis University, Budapest, Hungary.
authors.forename: G
authors.initials: G
authors.lastname: Péter
authors.forename: V
authors.initials: V
authors.lastname: Károly
authors.forename: B
authors.initials: B
authors.lastname: Imre
authors.forename: F
authors.initials: F
authors.lastname: János
authors.forename: Y
authors.initials: Y
authors.lastname: Kaneko
mesh_terms: D000920:Antibody-Dependent Cell Cytotoxicity / Q000187:drug effects; D003602:Cytotoxicity, Immunologic / Q000187:drug effects*; D006801:Humans; D007694:Killer Cells, Natural / Q000187:drug effects; D007912:Lentinan / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects* / Q000276:immunology; D011134:Polysaccharides / Q000494:pharmacology*
chemical_list: D011134:Polysaccharides; D007912:Lentinan