Ultrastructural analysis of the interaction between host cells and Trypanosoma cruzi in experimental chagomas.
The initial interaction between infective forms of Trypanosoma cruzi and host cells in vivo was studied at the ultrastructural level. In order to follow these events, T. cruzi bloodstream forms were inoculated into the cheek-pouch of hamsters--a peculiar region devoid of lymphatic vessels. This region was chosen as injection site because, unlike other regions, trypanosomes remained there and multiplied locally up to 15 days after inoculation. Parasites were detected initially outside cells or inside neutrophils. Only after the first week following inoculation were developing and multiplying trypanosomes seen inside macrophages or other resident cells. Parasites persisted until 15-20 days after inoculation, but by about the 28th day they were no longer seen.
Bijovsky
A T
AT
Departamento de Parasitologia, Universidade de São Paulo, Brazil.
Milder
R V
RV
D000818:Animals; D014355:Chagas Disease / Q000469:parasitology / Q000473:pathology; D006224:Cricetinae; D005260:Female; D006790:Host-Parasite Interactions; D008264:Macrophages / Q000469:parasitology* / Q000648:ultrastructure; D008297:Male; D008647:Mesocricetus; D008854:Microscopy, Electron; D008938:Mitosis; D009504:Neutrophils / Q000469:parasitology* / Q000648:ultrastructure; D013997:Time Factors; D014349:Trypanosoma cruzi / Q000648:ultrastructure*