title: Microbe-induced lymphocyte blastogenesis enhancement after preculture.
abstract: The in vitro blastogenic response of human peripheral blood mononuclear cells to Fusobacterium nucleatum and other oral microorganisms was enhanced if the peripheral blood mononuclear cells were cultured for 24 h at 37 degrees C prior to the addition of stimulant. The enhancement which occurred at optimal and supraoptimal concentrations of F. nucleatum (10 to 100 micrograms/ml) was detected after a preculture period of as little as 2 h. The blastogenic response was a result of T-cell proliferation, and enhancement occurred independently of monocytes. Suppressor activity was induced by culturing fresh lymphocytes for 24 h in the presence of supraoptimal concentrations of F. nucleatum. The enhancement phenomenon occurred independently of the prostaglandin effects on lymphocyte blastogenesis and was not abrogated by treatment with indomethacin.
authors.forename: D E
authors.initials: DE
authors.lastname: Lopatin
authors.forename: L M
authors.initials: LM
authors.lastname: Martel
authors.forename: D F
authors.initials: DF
authors.lastname: Mangan
mesh_terms: D000293:Adolescent; D000328:Adult; D002452:Cell Count; D005673:Fusobacterium / Q000502:physiology*; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology; D007700:Kinetics; D008213:Lymphocyte Activation; D008875:Middle Aged; D009000:Monocytes / Q000276:immunology; D009055:Mouth / Q000382:microbiology*; D013601:T-Lymphocytes / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology
chemical_list: D007213:Indomethacin