Images of purple membrane at 2.8 A resolution obtained by cryo-electron microscopy.
Improvements in technique have produced electron micrographs of purple membrane that provide, after computer analysis, reproducibly measurable diffraction peaks extending to 2.8 A (1 A = 0.1 nm). The improvements include better specimen preparation, a more stable cryo-electron microscope with better alignment and the addition of an image-processing step, which gives weights to local areas of the image according to the local strength of the periodic component of the image. These improvements have enabled the calculation of a directly phased projection map at 2.8 A resolution.
Baldwin
J M
JM
MRC Laboratory of Molecular Biology, Cambridge, U.K.
Henderson
R
R
Beckman
E
E
Zemlin
F
F
D001436:Bacteriorhodopsins / Q000032:analysis*; D005615:Freezing; D008854:Microscopy, Electron / Q000379:methods
D001436:Bacteriorhodopsins