A major role for protein kinase C in calcium-activated exocytosis in permeabilised adrenal chromaffin cells.
The role of endogenously activated protein kinase C in calcium-activated exocytosis was examined in digitonin-permeabilised bovine adrenal chromaffin cells. Protein kinase C activity was reduced by down-regulation following long-term treatment with PMA or by using the inhibitor sphingosine. Both treatments resulted in a substantial reduction in catecholamine secretion elicited by micromolar calcium, indicating that endogenous activation of protein kinase C is a major requirement for calcium-activated exocytosis in chromaffin cells.
Burgoyne
R D
RD
Physiological Laboratory, University of Liverpool, England.
Morgan
A
A
O'Sullivan
A J
AJ
D000313:Adrenal Medulla / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D002417:Cattle; D002463:Cell Membrane Permeability; D002478:Cells, Cultured; D005089:Exocytosis / Q000187:drug effects*; D007700:Kinetics; D011493:Protein Kinase C / Q000378:metabolism*; D013110:Sphingosine / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D011493:Protein Kinase C; D013110:Sphingosine; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium