Anhydrolevuglandin D2 inhibits the uterotonic activity of prostaglandins F2 alpha and D2.
Anhydrolevuglandin D2 (AnLGD2), which is produced from PGH2 by a water-induced rearrangement and subsequent dehydration, is uterotonic. However, increasing concentrations caused decreased responses of the uterine horns. AnLGD2 inhibited responses of uteri to stimulation by specific prostaglandins. PGF2 alpha was inhibited at an AnLGD2:PGF2 alpha ratio of 0.05:1 with 5 to 25 pg/ml concentrations of PGF2 alpha. The response to PGD2 was inhibited at an AnLGD2:PGD2 ratio of 0.05:1 with PGD2 concentrations of 5 to 75 pg/ml. In contrast, the uterotonic effects of PGE2 were not inhibited by AnLGD2. When AnLGD2 was added to baths with contracting uteri it inhibited contractions less if the exposure period was 5 min than if it was 10 min. The longer exposure times produced prolonged inhibition of contractile activity with bath concentrations of AnLGD2 as little as 2.5 pg/ml.
Foreman
D
D
Department of Biology, Case Western Reserve University, Cleveland, Ohio 44106.
Levison
B S
BS
Miller
D B
DB
Salomon
R G
RG
D000818:Animals; D015237:Dinoprost; D005260:Female; D066298:In Vitro Techniques; D007213:Indomethacin / Q000494:pharmacology; D015230:Prostaglandin D2; D011457:Prostaglandins D / Q000037:antagonists & inhibitors* / Q000494:pharmacology*; D011460:Prostaglandins F / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D051381:Rats; D014590:Uterine Contraction / Q000187:drug effects*
D011457:Prostaglandins D; D011460:Prostaglandins F; C056015:anhydrolevulgandin D2; D015237:Dinoprost; D015230:Prostaglandin D2; D007213:Indomethacin