title: The NADH-dehydrogenase subunit 5 gene in Oenothera mitochondria contains two introns and is co-transcribed with the 5 S rRNA gene.
abstract: The gene encoding subunit 5 of the NADH dehydrogenase complex (ND 5) has been identified in Oenothera mitochondria from a cDNA clone. The coding region is interrupted by a type II intron of 850 nucleotides and a second intervening sequence of 357 nucleotides. Genomic sequence rearrangement within the first intron creates a nontranscribed partial copy of the gene. The intact ND 5 gene is transcribed in a complex pattern with mRNAs including the 5 S rRNA sequence. Excision of the two introns appears to proceed slowly in vivo since the steady state mitochondrial RNA contains significant proportions of unprocessed precursor molecules.
authors.affiliation: Lehrstuhl für Spezielle Botanik, Universität Tübingen, Federal Republic of Germany.
authors.forename: B
authors.initials: B
authors.lastname: Wissinger
authors.forename: R
authors.initials: R
authors.lastname: Hiesel
authors.forename: W
authors.initials: W
authors.lastname: Schuster
authors.forename: A
authors.initials: A
authors.lastname: Brennicke
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003579:Cytochrome Reductases / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics*; D005796:Genes; D007438:Introns; D008928:Mitochondria / Q000032:analysis*; D008969:Molecular Sequence Data; D009245:NADH Dehydrogenase / Q000235:genetics*; D009690:Nucleic Acid Conformation; D010944:Plants / Q000201:enzymology / Q000235:genetics*; D012335:RNA, Ribosomal / Q000235:genetics*; D012341:RNA, Ribosomal, 5S / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D004272:DNA, Mitochondrial; D004275:DNA, Ribosomal; D012335:RNA, Ribosomal; D012341:RNA, Ribosomal, 5S; D003579:Cytochrome Reductases; D009245:NADH Dehydrogenase