title: A pentameric form of human serum amyloid P component. Crystallization, X-ray diffraction and neutron scattering studies.
abstract: Human serum amyloid P component crystallizes from sodium acetate buffer (pH 5.5) in the presence of calcium and polyethylene glycol 6000, at 4 degrees C. The space group is P2(1) and the cell parameters are a = 69.0 A (1 A = 0.1 nm), b = 99.3 A, c = 96.8 A, beta = 96.1. Density considerations supported by neutron scattering and gel filtration experiments indicate that the species crystallized is pentameric. The orientation of the pentamer 5-fold axis is determined and a crystal packing for the discs is proposed.
authors.affiliation: Department of Crystallography, Birkbeck College, London, U.K.
authors.forename: S P
authors.initials: SP
authors.lastname: Wood
authors.forename: G
authors.initials: G
authors.lastname: Oliva
authors.forename: B P
authors.initials: BP
authors.lastname: O'Hara
authors.forename: H E
authors.initials: HE
authors.lastname: White
authors.forename: T L
authors.initials: TL
authors.lastname: Blundell
authors.forename: S J
authors.initials: SJ
authors.lastname: Perkins
authors.forename: I
authors.initials: I
authors.lastname: Sardharwalla
authors.forename: M B
authors.initials: MB
authors.lastname: Pepys
mesh_terms: D003460:Crystallization; D006801:Humans; D046911:Macromolecular Substances; D009502:Neutrons; D012542:Scattering, Radiation; D000683:Serum Amyloid P-Component; D014961:X-Ray Diffraction
chemical_list: D046911:Macromolecular Substances; D000683:Serum Amyloid P-Component