High-performance-liquid-chromatographic analysis of radiolabelled prostaglandins and fatty acids with the radial compression column.
The method described is a rapid and reproducible separation of different prostanoids. With the use of a reversed phase 5 micron-radial compression column instead of a steel column-HPLC analysis is improved within a shorter time. In order to separate also the non-polar substances like arachidonic acid (AA) and hydroxy fatty acids in an appropriate time, a gradient-elution is used. The radioactivity is counted immediately after the column within a radioactivity monitor. With this system it is possible to examine the AA-metabolites enzymatically formed in cells or tissue from exogenous AA.
Wirthumer-Hoche
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C
Atherosclerosis Research Group (ASF), Vienna, Austria.
Virgolini
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I
Sinzinger
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H
D000368:Aged; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000302:isolation & purification* / Q000378:metabolism; D001792:Blood Platelets / Q000378:metabolism; D002250:Carbon Radioisotopes; D002853:Chromatography, Liquid / Q000379:methods; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D011453:Prostaglandins / Q000302:isolation & purification*; D014018:Tissue Distribution; D014316:Tritium
D001095:Arachidonic Acids; D002250:Carbon Radioisotopes; D011453:Prostaglandins; D014316:Tritium; D016718:Arachidonic Acid