Opsin for immunological studies.
Opsin for immunological studies can be prepared free from S-antigen by affinity chromatography on Con A-Sepharose. This preparation, however, contains 2-6% Con A originating from the affinity medium. Con A as impurity disturbs lymphocyte transformation tests carried out with opsin as test antigen. We describe a method for the removal of Con A by immuno-adsorption to Protein A/anti-Con A/IgG in detergent. In addition, we have selected some detergents and detergent concentrations in which opsin can be purified and added to the lymphocyte culture medium.
Broekhuyse
R M
RM
Kuhlmann
E D
ED
D000327:Adsorption; D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D003208:Concanavalin A / Q000032:analysis; D004340:Drug Contamination; D005136:Eye Proteins / Q000032:analysis / Q000302:isolation & purification*; D005779:Immunodiffusion; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000166:cytology; D017299:Rod Opsins
D005136:Eye Proteins; D017299:Rod Opsins; D003208:Concanavalin A