title: Molecular cloning of the barley seed protein CMd: a variant member of the alpha-amylase/trypsin inhibitor family of cereals.
abstract: The nucleotide and deduced amino-acid sequences of a cDNA clone encoding the barley seed protein CMd are described. The sequence is homologous with those of a family of inhibitors of alpha-amylase and trypsin, except for two short insertions. The longest of these (14 residues) is at the junction between the three proposed ancestral regions that comprise this family of proteins, and has limited identity with alpha-amylases of bacterial origin.
authors.affiliation: AFRC Institute of Arable Crops Research, Biochemistry Department, Rothamsted Experimental Station, Harpenden, U.K.
authors.forename: N G
authors.initials: NG
authors.lastname: Halford
authors.forename: N A
authors.initials: NA
authors.lastname: Morris
authors.forename: P
authors.initials: P
authors.lastname: Urwin
authors.forename: M S
authors.initials: MS
authors.lastname: Williamson
authors.forename: D D
authors.initials: DD
authors.lastname: Kasarda
authors.forename: E J
authors.initials: EJ
authors.lastname: Lew
authors.forename: M
authors.initials: M
authors.lastname: Kreis
authors.forename: P R
authors.initials: PR
authors.lastname: Shewry
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D002523:Edible Grain / Q000235:genetics*; D005796:Genes; D001467:Hordeum / Q000235:genetics*; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics*; D011500:Protein Synthesis Inhibitors / Q000235:genetics; D012639:Seeds / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid
chemical_list: C057341:CMd protein, Hordeum vulgare; D010940:Plant Proteins; D011500:Protein Synthesis Inhibitors; D004247:DNA