Heat stability of the extracellular lipase from a Pseudomonas strain isolated from refrigerated raw milk.
A study on the thermal stability of the extracellular lipase of a Pseudomonas strain has been made. The D-values at 140 degrees C ranged from 0.54 to 0.85 min, depending on the heating medium. The average Z-value and activation energy were 36.2 degrees C and 8.18 x 10(4) J. mol-1, respectively. The enzyme showed maximum activity at pH 8.5 and 37 degrees C.
García-Collía
P
P
Departamento de Higiene y Tecnologia de los Alimentos, Facultad de Veterinaria, Universidad Complutense, Madrid.
Sanz
B
B
García
M L
ML
Ordóñez
J A
JA
D000818:Animals; D001426:Bacterial Proteins / Q000302:isolation & purification*; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D008049:Lipase / Q000302:isolation & purification*; D008892:Milk / Q000382:microbiology*; D011489:Protein Denaturation; D011551:Pseudomonas fluorescens / Q000201:enzymology* / Q000302:isolation & purification
D001426:Bacterial Proteins; D008049:Lipase