Characterization of two distinct disulfide-linked B-G molecules in the chicken.
Alloantisera specific for B-G antigens recognized a complex of molecules of apparent molecular weights of 90 and 98 Kd under nonreducing conditions and molecules of 40, 44, and 48 Kd under reducing conditions on both embryo- and adult-derived peripheral red blood cells (RBC). The chicken B-G molecules produced a unique two-dimensional "diagonal" pattern. Two antisera permitted the characterization of the complex B-G molecular profile as a homodimer composed of 48-Kd subunits and as a heterodimer composed of 40- and 44-Kd subunits. A rabbit antiserum produced against B-G molecules preferentially recognized the 48-Kd reduced molecules, suggesting that the 90-Kd molecule was a homodimer composed of two 48-Kd molecules. One B-G reagent was capable of recognizing only the 98-Kd nonreduced B-G molecule that gave rise to 40- and 44-Kd molecules under reducing conditions, suggesting that the 98-Kd molecule was a heterodimer composed of 44- and 40-Kd subunits. Adult chicken B-G2 molecules produced a variety of two-dimensional isoelectric focusing/sodium dodecyl sulfate-polyacrylamide gel electrophoresis (IEF/SDS-PAGE) patterns depending on the characteristics of the reagent employed in the immunoprecipitation. B-G molecules were immunoprecipitated from primitive and definitive chicken RBCs but not from any nonerythroid cells tested. B-G molecules were not expressed by avian erythroblastosis virus (AEV)-transformed erythroleukemia cells, nor were they induced to appear with butyric acid-induced erythroid differentiation.
Kline
K
K
Division of Nutrition, University of Texas, Austin 78712.
Briles
W E
WE
Bacon
L
L
Sanders
B G
BG
D000818:Animals; D002461:Cell Line, Transformed; D002645:Chickens / Q000097:blood / Q000276:immunology*; D004220:Disulfides / Q000097:blood; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000276:immunology; D006649:Histocompatibility Antigens / Q000276:immunology*; D007106:Immune Sera; D007457:Iodine Radioisotopes; D010449:Peptide Mapping; D011233:Precipitin Tests; D012697:Serine Endopeptidases
D004220:Disulfides; D006649:Histocompatibility Antigens; D007106:Immune Sera; D007457:Iodine Radioisotopes; D012697:Serine Endopeptidases; C028344:glutamyl endopeptidase