Immunization of mice with the fibronectin-binding protein and clumping factor from Staphylococcus aureus: antibody response and resistance against intraperitoneal infection.
The antibody response against Staphylococcus aureus antigens was quantitated in mice immunized with live staphylococci, the fibronectin-binding protein, clumping factor, or saline by means of enzyme-linked immunosorbent assay. Immunization with purified clumping factor induced a significant increase in antibody levels, while the fibronectin-binding protein gave a poor response. All mice immunized with live S. aureus developed a high antibody level. The immunization of mice with live S. aureus or clumping factor resulted in an increased resistance towards staphylococcal intraperitoneal infection.
Espersen
F
F
Clemmensen
I
I
D000818:Animals; D000917:Antibody Formation; D000942:Antigens, Bacterial; D003030:Coagulase / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005353:Fibronectins / Q000276:immunology*; D007116:Immunization, Passive; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D017469:Receptors, Fibronectin; D011971:Receptors, Immunologic / Q000276:immunology*; D013203:Staphylococcal Infections / Q000276:immunology*; D013211:Staphylococcus aureus / Q000276:immunology*
D000942:Antigens, Bacterial; D003030:Coagulase; D005353:Fibronectins; D017469:Receptors, Fibronectin; D011971:Receptors, Immunologic