Sex selection with monoclonal H-Y antibody.
H-Y antigen defined by antibody from male-sensitized female mice has been reported in male embryos of mouse, rat, cattle, goat, pig, and sheep. We now describe the use of monoclonal H-Y antibodies in identification of male and female during embryo transfer in cattle. Monoclonal H-Y antibodies were applied with fluorescein-isothiocyanate-conjugated goat antimouse immunoglobulin (Ig), and the embryos were scored for fluorescence under ultraviolet light. In alternative trials with 149 embryos, the method could be applied with 73% to 82% efficiency. Pregnancy rates for treated embryos were at least as high as those expected for untreated embryos. The calves that developed from the treated embryos are normal and growing.
Wachtel
S
S
Center for Reproductive Biology, Collierville, Tennessee.
Nakamura
D
D
Wachtel
G
G
Felton
W
W
Kent
M
M
Jaswaney
V
V
D000818:Animals; D000911:Antibodies, Monoclonal; D002417:Cattle; D004622:Embryo, Mammalian; D005260:Female; D005434:Flow Cytometry; D005818:Genetic Engineering / Q000662:veterinary*; D006182:H-Y Antigen / Q000276:immunology*; D008297:Male; D012743:Sex Preselection / Q000379:methods / Q000662:veterinary*
D000911:Antibodies, Monoclonal; D006182:H-Y Antigen