Heterogeneity of class I beta-lactamase expression in clinical isolates of Pseudomonas aeruginosa.
Expression of chromosomal beta-lactamase was examined in 85 clinical isolates of Pseudomonas aeruginosa. beta-Lactamase assays with and without cefoxitin induction revealed four phenotypes of enzyme expression: low basal, inducible; moderate basal, inducible; moderate basal, constitutive; and high basal, constitutive. The isoelectric points of the major beta-lactamase bands were 9.4, 9.2, and 8.4. These results indicate that there is a limited heterogeneity in expression of chromosomal beta-lactamase of P. aeruginosa.
Sanders
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Department of Medical Microbiology, Creighton University School of Medicine, Omaha, Nebraska 68178.
Gates
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Sanders
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D000900:Anti-Bacterial Agents / Q000494:pharmacology; D004352:Drug Resistance, Microbial; D004794:Enzyme Repression; D010405:Penicillinase / Q000096:biosynthesis*; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000201:enzymology*
D000900:Anti-Bacterial Agents; D010405:Penicillinase