Hereditary heat-labile hexosaminidase B: a variant whose homozygotes synthesize a functional HEX A.
Homozygosity for a mutant allele at the beta-chain locus of hexosaminidase (HEX), resulting in a variant of heat-labile HEX B, is reported for the first time in two healthy children. HEX activity in their sera, leukocytes, and cultured skin fibroblasts is severely deficient when measured on the synthetic substrate 4-MU-GLcNAc. However, their cultured skin fibroblasts synthesize and process both alpha and beta chains of HEX, and their lymphoid cells hydrolyze normally the natural ganglioside GM2. This mutation is, therefore, different from at least one of the beta-chain mutations found in previously published families with heat-labile HEX B.
Navon
R
R
Kopel
R
R
Nutman
J
J
Frisch
A
A
Conzelmann
E
E
Sandhoff
K
K
Adam
A
A
D002478:Cells, Cultured; D002648:Child; D002852:Chromatography, Ion Exchange; D005260:Female; D005347:Fibroblasts / Q000201:enzymology; D005678:G(M2) Ganglioside / Q000378:metabolism; D054818:Hexosaminidase A; D054819:Hexosaminidase B; D006596:Hexosaminidases / Q000096:biosynthesis / Q000235:genetics*; D006720:Homozygote; D006358:Hot Temperature; D006801:Humans; D007223:Infant; D007527:Isoenzymes / Q000096:biosynthesis / Q000235:genetics*; D008214:Lymphocytes / Q000378:metabolism; D008297:Male; D009154:Mutation; D010375:Pedigree; D013661:Tay-Sachs Disease / Q000201:enzymology / Q000235:genetics; D001619:beta-N-Acetylhexosaminidases
D007527:Isoenzymes; D005678:G(M2) Ganglioside; D006596:Hexosaminidases; D054818:Hexosaminidase A; D054819:Hexosaminidase B; D001619:beta-N-Acetylhexosaminidases