[Laser fluorimetry of NADH].
Laser fluorimetry of reduced nicotinamide adenine-dinucleotide (NADH) in situ is a new technique used for real-time studies of the degree of reduction of the first link in the mitochondrial respiration chain. We present here the first results obtained in animal experiments and in clinical exploration: In rats, coronary occlusion produces a substantial rise in mitochondrial NADH, followed by a fall below basal level during reperfusion. Tetanic contraction of slow-twitch muscles in rats results in a rise in NADH level higher than that produced by contraction of fast-twitch muscles. During coronary angiography, injection of the contrast medium induces a rise in NADH in patients with a significant degree of coronary stenosis. Intravenous nitroglycerin reduces both left ventricular end-diastolic pressure and NADH fluorescence concomitantly, due to preferential distribution of the coronary blood flow to sub-endocardial layers. Exercise-induced ischaemia results in a decrease of muscular NADH concentration in Mc Ardle syndrome, due to phosphorylase deficiency.
Toussaint
M
M
Service des maladies cardio-vasculaires, Hôpital Cochin, Paris.
Duboc
D
D
Renault
G
G
Guerin
F
F
Degeorges
M
M
D000818:Animals; D017023:Coronary Angiography; D003327:Coronary Disease / Q000378:metabolism; D004734:Energy Metabolism; D005470:Fluorometry; D006012:Glycogen Storage Disease Type V / Q000378:metabolism; D006801:Humans; D007834:Lasers; D008929:Mitochondria, Heart / Q000378:metabolism; D015425:Myocardial Reperfusion; D009206:Myocardium / Q000378:metabolism*; D009243:NAD / Q000032:analysis*; D005996:Nitroglycerin / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains
D009243:NAD; D005996:Nitroglycerin
Fluorimétrie laser du NADH.