Electrophoretic mobility studies on doxorubicin-resistant and -sensitive murine P388 leukemic cells.
The electrokinetic properties of doxorubicin (DOX)-resistant (P388/R) and -sensitive (P388/S) murine leukemic cells were studied in a free-flow electrophoresis (FFE) system. The electrophoretic mobilities (EM) of P388/S and P388/R cells were 1.07 and 1.35 x 10(-4) cm2 V-1 s-1, respectively, suggesting a higher net negative charge on the P388/R cells. Neuraminidase treatment decreased the EM of both the P388/S and P388/R cells by 15-20% but had no effect on cellular doxorubicin retention. Total and cell surface sialic acid contents were similar in both the cell lines. Our studies show that no direct correlations may exist among surface charge, cell surface sialic acid content, and doxorubicin retention in DOX-resistant and -sensitive P388 cells; however, differences in cell surface charge between these cell types were used to separate them by preparative FFE.
Nair
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Division of Cytokinetics, Papanicolaou Comprehensive Cancer Center, Miami, Florida.
Horton
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A
Leif
R C
RC
Krishan
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A
D000818:Animals; D004317:Doxorubicin / Q000378:metabolism* / Q000494:pharmacology; D004351:Drug Resistance; D004563:Electrochemistry; D004586:Electrophoresis; D005434:Flow Cytometry; D006801:Humans; D007941:Leukemia P388 / Q000378:metabolism*; D007942:Leukemia, Experimental / Q000378:metabolism*; D007945:Leukemia, Lymphoid / Q000378:metabolism; D051379:Mice; D019158:N-Acetylneuraminic Acid; D009439:Neuraminidase / Q000494:pharmacology; D012794:Sialic Acids / Q000032:analysis; D013499:Surface Properties; D014407:Tumor Cells, Cultured
D012794:Sialic Acids; D004317:Doxorubicin; D009439:Neuraminidase; D019158:N-Acetylneuraminic Acid