Structure elucidation of the blood group B like and blood group I active octaantennary ceramide tetracontasaccharide from rabbit erythrocyte membranes by two-dimensional 1H NMR spectroscopy at 600 MHz.
The primary structure of the ceramide tetracontasaccharide (1) from rabbit erythrocyte membranes has been determined with the aid of 600-MHz two-dimensional phase-sensitive correlated, "totally correlated" (TOCSY, homonuclear Hartmann-Hahn), relayed coherence transfer, triple quantum filtered, and nuclear Overhauser enhancement 1H NMR spectra. It was shown that obtaining subspectra of the constituent sugar residues from a totally correlated spectrum and assigning the resonances occurring in these subspectra by analyzing the relevant cross-peaks in phase-sensitive correlated spectra is the most efficient way for establishing complex oligosaccharide structures. This analysis has shown 1 to be the highest homologue of the multiantennary neolactoglycosphingolipids of the following general formula with n = 7: (Formula: see text).
Dabrowski
J
J
Max-Planck-Institut für Medizinische Forschung, Heidelberg, West Germany.
Dabrowski
U
U
Kordowicz
M
M
Hanfland
P
P
D000017:ABO Blood-Group System; D000818:Animals; D001789:Blood Group Antigens; D002554:Cerebrosides / Q000097:blood*; D004910:Erythrocyte Membrane / Q000032:analysis*; D007048:I Blood-Group System; D009682:Magnetic Resonance Spectroscopy; D011817:Rabbits
D000017:ABO Blood-Group System; D001789:Blood Group Antigens; D002554:Cerebrosides; D007048:I Blood-Group System; C057448:ceramide tetracontasaccharide