Simple high-performance liquid chromatographic method for the measurement of amiloride in body fluids.
A simplified rapid high-performance liquid chromatographic (HPLC) procedure has been developed for the measurement of amiloride in plasma or urine. Solid-phase extraction columns provide quick, clean and simple sample preparation, allowing ten samples to be processed in less than 5 min. The HPLC system uses a standard reversed-phase (C18) column with detection by ultraviolet absorption at 365 nm. The assay has been used to define plasma amiloride concentration-time profiles and to quantitate urine amiloride recovery in healthy men after repeated administration at two doses.
Forrest
G
G
University Department of Medicine, Gardiner Institute, Western Infirmary, Glasgow, Scotland, U.K.
McInnes
G T
GT
Fairhead
A P
AP
Thompson
G G
GG
Brodie
M J
MJ
D000328:Adult; D000584:Amiloride / Q000032:analysis* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D008297:Male
D000584:Amiloride