Comparison of scanning and transmission electron microscopy of the epithelial pocket wall in juvenile and adult periodontitis.
Using scanning (SEM) and transmission (TEM) electron microscopy, this study compared fine structural features of the pocket walls in both juvenile and adult periodontitis (JP and AP, respectively) in 40 cases. Gingiva was also obtained from a control group consisting of periodontally noninvolved teeth. Clinical parameters were assessed in both JP and AP patients as well as in controls. Clinical findings showed low plaque accumulation, marked periodontal tissue destruction and less gingival inflammation in JP. Bone destruction and attachment loss were more marked in JP than in AP. AP had a higher plaque index and more evident gingival inflammation. SEM observations of JP as compared to AP showed gross distortions in pocket walls, an increased beaded appearance of microridges, and separation between pocket epithelial cells. TEM showed partially desquamated and separated superficial epithelial cells, but only in JP were fine granular precipitates observed in the intercellular spaces. The observations demonstrated structural features indicative of more prominent degenerative changes in JP than in AP. Also, these features were coincidental with a higher plaque index in AP than in JP, where clinical features (including a low plaque index) were not proportional to the epithelial destructive changes present.
Shafik
S S
SS
Department of Periodontics, Tanta University, Egypt.
Zaki
A E
AE
Ashrafi
S H
SH
Nour
Z M
ZM
ElNesr
N M
NM
D000293:Adolescent; D000328:Adult; D010520:Aggressive Periodontitis / Q000473:pathology*; D000539:Alveolar Process / Q000648:ultrastructure; D004846:Epithelial Attachment / Q000648:ultrastructure; D004848:Epithelium / Q000648:ultrastructure; D005260:Female; D006801:Humans; D008297:Male; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D010510:Periodontal Diseases / Q000473:pathology*; D010514:Periodontal Pocket / Q000473:pathology*; D010518:Periodontitis / Q000473:pathology*