An immunological assessment of lysosomal enzymes and other macromolecules sulfated during vegetative growth of Dictyostelium discoideum.
Western blotting and immunoprecipitation data indicated that lysosomal enzymes represent a subset of the sulfated macromolecules present in vegetative Dictyostelium discoideum amoebae and account for less than 2.5% of the total sulfate incorporated during vegetative growth. These data suggest that the majority of the highly sulfated macromolecules of vegetative D. discoideum amoebae are not related to the lysosomal enzymes.
Davis
S J
SJ
School of Biological Sciences, Flinders University of South Australia, Bedford Park.
Wheldrake
J F
JF
Freeze
H H
HH
D000118:Acetylglucosaminidase / Q000032:analysis; D000486:Allergy and Immunology; D004023:Dictyostelium / Q000254:growth & development*; D007124:Immunoenzyme Techniques; D046911:Macromolecular Substances / Q000032:analysis*; D008361:Mannosidases / Q000032:analysis; D013431:Sulfates / Q000378:metabolism; D001617:beta-Glucosidase / Q000032:analysis
D046911:Macromolecular Substances; D013431:Sulfates; D008361:Mannosidases; D001617:beta-Glucosidase; D000118:Acetylglucosaminidase