title: The effect of selected arachidonic acid metabolites on natural killer cell activity.
abstract: The effect of arachidonic acid (AA) metabolites of lipoxygenase(s) was evaluated on natural killer (NK) cell activity in Fischer F344 rat splenic lymphocytes and compared with prostaglandin E2 (PGE2), a known inhibitor of NK cell lytic activity. It was observed that 5(S),12(S)-dihydroxy-6,10-trans-8,14-cis-eicosatetraenoic acid (5(S),12(S)-diHETE, EZEZ) inhibited NK cell activity to a degree comparable to the inhibitory effects of PGE2. This compound maximally inhibited NK cell activity at concentrations of 10(-6) and 10(-8) M. PGE2 and 5(S),12(S)-diHETE (EZEZ) inhibited NK activity to an identical degree at all concentrations and effector:target (E:T) cell ratios tested. Of the other lipoxygenase pathway metabolites screened, 8(S),15(S)-all trans-diHETE and 8(S),15(S)-diHETE (EZEZ) also inhibited NK activity, but only at 10(-6) M and a 50:1 E:T cell ratio. These findings provide further evidence that the lipoxygenase and cyclooxygenase pathways produce metabolites which can modulate NK cell function, and that 5(S),12(S)-diHETE (EZEZ), which has not been previously tested for effects on NK cells, may have a significant immunoregulatory role.
authors.affiliation: Pennsylvania State University, Department of Veterinary Science, University Park 16802.
authors.forename: W E
authors.initials: WE
authors.lastname: Field
authors.forename: F G
authors.initials: FG
authors.lastname: Ferguson
authors.forename: P
authors.initials: P
authors.lastname: Reddanna
authors.forename: C C
authors.initials: CC
authors.lastname: Reddy
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001095:Arachidonic Acids / Q000378:metabolism*; D015232:Dinoprostone / Q000494:pharmacology; D066298:In Vitro Techniques; D007694:Killer Cells, Natural / Q000187:drug effects*; D008084:Lipoxygenase / Q000494:pharmacology* / Q000502:physiology; D008297:Male; D051381:Rats; D011916:Rats, Inbred F344
chemical_list: D001095:Arachidonic Acids; D008084:Lipoxygenase; D015232:Dinoprostone