H-2-linked Ir genes have a striking influence on the immunogenicity of idiotopes of myeloma protein 315 for T helper cells.
H-2-linked immune response (Ir) genes control T helper cells (Th) that recognize idiotopes of the V domains of myeloma protein 315 as carriers; Th recognition was detected by augmentation of antibody responses of hapten (4-hydroxy-3-iodo-5-nitrophenylacetyl (NIP]-primed B cells boosted with NIP conjugated to Fab315. The present study indicates that the responder k allele of the Ir VH315 gene maps to the I-A subregion of H-2. A responder s allele of the Ir V lambda 2(315) gene on an A-strain background was identified, which also most likely maps to I-A. Although the d allele of the Ir V lambda 2(315) gene is a responder allele on DBA/2 background, the D2.GD strain (with I-region haplotype AdBbJbEbCb) was non-responder to V lambda 2(315), suggesting either that the responder d allele maps to I-E or that the b allele of a second Ir V lambda 2(315) gene located to the right of I-A exerts a strong suppressive influence. The H-2b haplotype conferred non-responsiveness to VH315, V lambda 2(315), and Fv315.
Jørgensen
T
T
Bogen
B
B
Hannestad
K
K
D000818:Animals; D002874:Chromosome Mapping; D005802:Genes, MHC Class II; D006183:H-2 Antigens / Q000235:genetics* / Q000276:immunology; D006238:Haploidy; D000949:Histocompatibility Antigens Class II / Q000235:genetics / Q000276:immunology; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D007135:Immunoglobulin Variable Region / Q000235:genetics; D007146:Immunoglobulin lambda-Chains / Q000235:genetics; D051379:Mice; D008805:Mice, Inbred A; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D009194:Myeloma Proteins / Q000008:administration & dosage / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*
D006183:H-2 Antigens; D000949:Histocompatibility Antigens Class II; D007143:Immunoglobulin Heavy Chains; D007130:Immunoglobulin Idiotypes; D007135:Immunoglobulin Variable Region; D007146:Immunoglobulin lambda-Chains; D009194:Myeloma Proteins