title: Protein kinase C activity in compensatory kidney growth.
abstract: Very little information exists about the intracellular mechanisms mediating remnant kidney hypertrophy after reduction of renal mass. The present experiments demonstrate that the activity of a key enzyme in cellular proliferation, protein kinase C, is enhanced in the kidney during post-uninephrectomy hypertrophy. As additional evidence of "in vivo" enzyme activation, the enzyme activity migrates from the cytosolic to the particulate fraction of the cells and the number of sites of phorbol dibutyrate binding is enhanced in kidney membranes 24 hr after contralateral nephrectomy. The stimulation of the enzyme is higher than the increase in kidney weight, although both phenomena are linearly related.
authors.affiliation: Department of Medicine, University of Colorado School of Medicine, Denver 80262.
authors.forename: C
authors.initials: C
authors.lastname: Caramelo
authors.forename: P
authors.initials: P
authors.lastname: Tsai
authors.forename: K
authors.initials: K
authors.lastname: Okada
authors.forename: R W
authors.initials: RW
authors.lastname: Schrier
mesh_terms: D000818:Animals; D029742:Caenorhabditis elegans Proteins; D002352:Carrier Proteins; D006984:Hypertrophy; D007668:Kidney / Q000201:enzymology* / Q000473:pathology; D007700:Kinetics; D009392:Nephrectomy; D015240:Phorbol 12,13-Dibutyrate; D010703:Phorbol Esters / Q000378:metabolism; D011493:Protein Kinase C / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D011955:Receptors, Drug
chemical_list: D029742:Caenorhabditis elegans Proteins; D002352:Carrier Proteins; D010703:Phorbol Esters; D011955:Receptors, Drug; C083141:phorbol ester binding protein; C027776:phorbol ester receptor; D015240:Phorbol 12,13-Dibutyrate; D011493:Protein Kinase C