Intrinsic and extrinsic labeling for studies of manganese absorption in humans.
A dual-radioisotope method was used to simultaneously study whole-body manganese retention from a chicken liver based meal intrinsically labeled with 54Mn and extrinsically labeled with 52Mn. Manganese retention was monitored in a sensitive whole-body counter during approximately 30 d in six young adult women. Both radioisotopes were retained to a similar degree and excreted at identical rates. Retention at d 5 was 14.4 +/- 10.3 and 14.0 +/- 9.9% while retention at d 10 was 5.0 +/- 3.1 and 5.0 +/- 3.0% (X +/- SD) for 54Mn and 52Mn, respectively. From these results we conclude that the intrinsic and extrinsic Mn isotopes did form a common pool before absorption. The results can therefore be regarded as a direct validation of the use of extrinsic labeling for studies of Mn retention for estimating Mn absorption in man.
Davidsson
L
L
Department of Clinical Nutrition, University of Gothenburg, Sahlgrenska Hospital, Sweden.
Cederblad
A
A
Hagebø
E
E
Lönnerdal
B
B
Sandström
B
B
D000328:Adult; D004032:Diet; D005260:Female; D005504:Food Analysis; D006801:Humans; D007408:Intestinal Absorption; D007501:Iron / Q000097:blood; D007553:Isotope Labeling; D008345:Manganese / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics*; D011868:Radioisotopes / Q000008:administration & dosage; D014915:Whole-Body Counting / Q000379:methods
D011868:Radioisotopes; D008345:Manganese; D007501:Iron