[Inhibition of the antioxidative activity of plasma by sodium azide].
Powerful antioxidant activity of human plasma was demonstrated by measuring the thiobarbituric acid reaction and Fe+2-induced chemiluminescence. Inhibition of lipid peroxidation was shown both for plasma lipids and for the suspension of egg lipoproteins, which was taken as a model system. The inhibitory effect of plasma peroxidation was removed by azide Na taken in the concentration of 0.5 mg/ml, but caeroplasmin activity in the plasma was completely suppressed at NaN3 concentration equal to 0.1 mg/ml. A low correlation (r = 0.75) between caeruloplasmin activity in the blood plasma and extent of chemiluminescence activation obtained in the presence of NaN3 was found. The presented data led to an assumption that only a part of lipid peroxidation inhibitors in the plasma can be attributed with caeruloplasmin.
Klebanov
G I
GI
Teselkin
Iu O
IuO
Vladimirov
Iu A
IuA
D001055:Apolipoproteins B / Q000097:blood*; D001386:Azides / Q000494:pharmacology*; D002570:Ceruloplasmin / Q000378:metabolism; D003864:Depression, Chemical; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects*; D049449:Luminescence; D010084:Oxidation-Reduction; D019810:Sodium Azide
D001055:Apolipoproteins B; D001386:Azides; D019810:Sodium Azide; D002570:Ceruloplasmin
Ingibirovanie antiokislitel'noĭ aktivnosti plazmy krovi azidom natriia.