title: Isolation of T lymphocyte subsets from peripheral blood using monoclonal antilymphocyte antibodies.
abstract: Monoclonal antisera against helper (serum OKT4) and suppressor (serum OKT8) T cells were employed in a complement-dependent lymphocytotoxicity reaction to isolate helper and suppressor T cells from peripheral blood mononuclear cells. When prepared by this method, helper and suppressor T cells had a purity of 67.8 percent and 78.8 percent, respectively. Approximately 56 percent of viable suppressor and 33 percent of viable helper T cells were lost during the procedure.
authors.forename: R
authors.initials: R
authors.lastname: Patel
authors.forename: A
authors.initials: A
authors.lastname: Soriano
authors.forename: L
authors.initials: L
authors.lastname: Loeb
authors.forename: M
authors.initials: M
authors.lastname: Ghoneum
authors.forename: D
authors.initials: D
authors.lastname: Najera
mesh_terms: D000911:Antibodies, Monoclonal; D006801:Humans; D013601:T-Lymphocytes / Q000145:classification*; D006377:T-Lymphocytes, Helper-Inducer; D050378:T-Lymphocytes, Regulatory
chemical_list: D000911:Antibodies, Monoclonal