Structure of 6-deoxytalose-containing polysaccharide from the cell wall of Bifidobacterium adolescentis.
The isolation and analysis of the cell wall and the polysaccharide-glycopeptide complexes of Bifidobacterium adolescentis YIT4011 are presented. Polysaccharide-glycopeptide complexes, PS-GP1 and PS-GP2, were solubilized from the cell wall by treatment with N-acetylmuramidase. PS-GP1 and PS-GP2 were found to be composed of glucose, 6-deoxytalose and a small amount of glycopeptide. The products of Smith degradation of the PS-GPs had no glucose-containing fraction, but were composed of 1,2/1,3-linked 6-deoxytalose. Furthermore, a second Smith degradation of this fraction yielded trisaccharide-glyceraldehyde. These results and methylation analysis led to the conclusion that PS-GP1 or 2 has a repeating unit of----3)6dTal(beta 1----3)6dTal(beta 1----3)6dTal(beta 1----2)-6dTal(alpha 1----2)6dTal(alpha 1----2)6dTal(alpha 1-, and that glucose residues are linked to position C-3 of the 2-O-substituted 6-deoxytalose residues.
Nagaoka
M
M
Yakult Central Institute for Microbiological Research, Kunitachi, Tokyo.
Muto
M
M
Yokokura
T
T
Mutai
M
M
D001644:Bifidobacterium / Q000032:analysis*; D002240:Carbohydrate Sequence; D002473:Cell Wall / Q000032:analysis; D055598:Chemical Phenomena; D002621:Chemistry; D002854:Chromatography, Paper; D003837:Deoxy Sugars / Q000032:analysis; D006020:Glycopeptides / Q000302:isolation & purification; D006601:Hexoses; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D011135:Polysaccharides, Bacterial / Q000302:isolation & purification
D003837:Deoxy Sugars; D006020:Glycopeptides; D006601:Hexoses; D011135:Polysaccharides, Bacterial; C011754:6-deoxytalose