Micro-analysis of mineral saturation within enamel during lactic acid demineralization.
In this study, the physicochemical factors responsible for caries-like lesion propagation were investigated by means of a micro-analytical system used to study the fluid within a lesion during a simulation of the decay process. Four 500-microns-thick serial sections prepared from a single human molar were mounted between glass plates with only the natural surface of the tooth exposed. Microwells were then drilled into sound and pre-existing carious regions of the section through one of the plates. These microwells were then filled with fluid under mineral oil, and after a week of equilibration, the natural surface of the section was exposed to a lesion-producing fluid. The concentrations of calcium, phosphate, and hydrogen ions of the fluid in the wells were then followed as a function of time as the lesion advanced. The results of this study, in which lactic acid was used to demineralize enamel, were consistent with those previously reported (Vogel et al., 1987a): The solution within the lesion remained saturated during the acid attack. Differences in initial mobilities of the calcium and phosphate and other ions, a result of the permselectivity of the enamel, increased the concentrations within the lesion and permanently changed the ratio of these ions in the lesion solution. Based on these results, we suggest that the ionic permselectivity of tooth enamel can have a profound effect on the transport of mineral from a caries lesion.
Vogel
G L
GL
Paffenbarger Research Center, American Dental Association Health Foundation, National Bureau of Standards, Gaithersburg, Maryland 20899.
Carey
C M
CM
Chow
L C
LC
Gregory
T M
TM
Brown
W E
WE
D002118:Calcium / Q000032:analysis; D003124:Colorimetry / Q000295:instrumentation; D003731:Dental Caries / Q000378:metabolism*; D003743:Dental Enamel / Q000032:analysis*; D003745:Dental Enamel Permeability / Q000187:drug effects; D006801:Humans; D006859:Hydrogen / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D007773:Lactates / Q000494:pharmacology*; D019344:Lactic Acid; D008839:Microelectrodes; D008903:Minerals / Q000032:analysis*; D010710:Phosphates / Q000032:analysis; D013053:Spectrophotometry
D007773:Lactates; D008903:Minerals; D010710:Phosphates; D019344:Lactic Acid; D006859:Hydrogen; D002118:Calcium