[Humoral response in Leishmania infantum clinical infections].
We sought a specific humoral response in man in order to find an antigenic marker of Leishmania infantum infection. This study was carried out by the Western blot technique; 42 sera from human patients were analysed on a standard electrophoretic pattern (SDS-PAGE) of L. infantum antigens. Patient sera reacted specifically with 4 (160-, 140-, 94- and 79-Kd) antigens, and reactive antibodies were IgG. We suggest that these antigens may be used for diagnosis of L. infantum visceral leishmaniasis.
Berneman
A
A
Institut de Technologie des surfaces actives, Université de Technologie de Compiègne, France.
Rolland
X
X
Broun
G
G
D000818:Animals; D000913:Antibodies, Protozoan / Q000096:biosynthesis* / Q000302:isolation & purification; D000953:Antigens, Protozoan; D015415:Biomarkers; D015153:Blotting, Western; D006801:Humans; D007893:Leishmania donovani / Q000276:immunology*; D007898:Leishmaniasis, Visceral / Q000175:diagnosis / Q000276:immunology*
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D015415:Biomarkers
La réponse humorale chez l'homme dans les infections à Leishmania infantum.