title: Proliferation and cell loss of human leukemic cell subpopulations in liquid culture.
abstract: A kinetic study was performed on leukemic blasts from patients with acute myeloid leukemia, separated into 2 subpopulations by a specific density gradient. The growth curve and the [3H]-thymidine uptake were simultaneously analyzed. While cumulative nucleotide uptake fitted with the growth kinetics in the low-density fraction, such a concordance was not found in the high-density subpopulation. That indicated the occurrence of simultaneous growth and loss in the high density fraction, which could not be evaluated by a simple numerical determination.
authors.affiliation: Div. Ematologia, Università degli Studi, Policlinico di Careggi, Firenze (Italy).
authors.forename: P A
authors.initials: PA
authors.lastname: Bernabei
authors.forename: F C
authors.initials: FC
authors.lastname: Agostino
authors.forename: R
authors.initials: R
authors.lastname: Bezzini
authors.forename: R
authors.initials: R
authors.lastname: Saccardi
authors.forename: V
authors.initials: V
authors.lastname: Gattei
authors.forename: V
authors.initials: V
authors.lastname: Santini
authors.forename: M
authors.initials: M
authors.lastname: Casini
authors.forename: P
authors.initials: P
authors.lastname: Rossi Ferrini
mesh_terms: D002455:Cell Division; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000473:pathology*; D014407:Tumor Cells, Cultured / Q000473:pathology*