title: Restoration of Ca2+-activated tension of CDTA-treated single skeletal muscle fibers by troponin C.
abstract: Single fibers from glycerinated rabbit psoas muscle were treated with a solution containing CDTA, a strong chelator of metal ions. The CDTA-treated fibers lost all of the troponin C and showed no Ca2+-activated tension development. The addition of troponin C restored the Ca2+-activated tension of CDTA-treated fibers. The tension-pCa relationship in the case of the CDTA-treated fibers reconstituted with troponin C was almost the same as that in the case of the same fibers before the CDTA treatment. These results are consistent with those of the previous study on the Ca2+-activated ATPase of CDTA-treated rabbit skeletal myofibrils.
authors.affiliation: Department of Pharmacology, Faculty of Medicine, Kyushu University, Fukuoka.
authors.forename: S
authors.initials: S
authors.lastname: Morimoto
authors.forename: T
authors.initials: T
authors.lastname: Fujiwara
authors.forename: I
authors.initials: I
authors.lastname: Ohtsuki
mesh_terms: D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002614:Chelating Agents / Q000494:pharmacology; D004492:Edetic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D066298:In Vitro Techniques; D009119:Muscle Contraction / Q000187:drug effects*; D009132:Muscles / Q000187:drug effects / Q000378:metabolism; D009218:Myosins / Q000494:pharmacology; D011817:Rabbits; D014336:Troponin / Q000378:metabolism / Q000494:pharmacology*; D019209:Troponin C
chemical_list: D002614:Chelating Agents; D014336:Troponin; D019209:Troponin C; C005390:CDTA; D004492:Edetic Acid; D009218:Myosins; D002118:Calcium