[A new sensitivity test for substances used as antiseptics or disinfectants. I. Biological studies].
In this first article, the authors present a fast method of determining the bactericide and bacteriostatic power of the germs, based on the disparition of the succino-dehydrogenase activity of a bacterium, objectified by a neotetrazolium chloride reaction. This reaction allows a colorimetric qualitative and quantitative determination of the activity of a preparation and the duration of its contact with the bacteria. This method allows a study in electronic microscopy which will be published in a second article.
Dodin
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A
Laboratoire d'Ecologie bactérienne, Institut Pasteur, Paris.
Ryter
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A
Nicolas
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R
D000891:Anti-Infective Agents, Local / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects* / Q000201:enzymology; D003124:Colorimetry / Q000379:methods; D004202:Disinfectants / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000201:enzymology; D012486:Salmonella typhimurium / Q000187:drug effects / Q000201:enzymology; D013211:Staphylococcus aureus / Q000187:drug effects / Q000201:enzymology; D013385:Succinate Dehydrogenase / Q000378:metabolism; D013778:Tetrazolium Salts; D013997:Time Factors; D014734:Vibrio cholerae / Q000187:drug effects / Q000201:enzymology
D000891:Anti-Infective Agents, Local; D004202:Disinfectants; D013778:Tetrazolium Salts; C011314:neotetrazolium; D013385:Succinate Dehydrogenase
Pour un nouveau test de sensibilité aux substances données pour antiseptiques ou désinfectantes. I. Etudes biologiques.