[Micromodification of the determination of the ATPase activity of tissues and their biostructures by using malachite green].
A modified procedure for microestimation of the ATPase activity in tissues and their biostructures is described. Inorganic phosphate liberated during hydrolysis of ATP was determined using a set of reagents involving ammonium molybdate and malachite green. Biological samples containing small amount of material (by weight and volume) were analyzed by means of the procedure, which proved to be economical, reproducible and rapid. Results of the ATPase activity estimation in heart muscle homogenate and in other tissues and soluble structures carried out by means of the procedure were similar to the data on the enzymatic activity determined by other methods.
Gribanov
G A
GA
Shchennikova
V V
VV
Fedorovskiĭ
I V
IV
D000251:Adenosine Triphosphatases / Q000032:analysis*; D000818:Animals; D001205:Ascorbic Acid; D017301:Ca(2+) Mg(2+)-ATPase; D004396:Coloring Agents; D008297:Male; D008722:Methods; D008928:Mitochondria / Q000201:enzymology; D009206:Myocardium / Q000201:enzymology; D051381:Rats; D012394:Rosaniline Dyes; D013737:Testis / Q000201:enzymology
D004396:Coloring Agents; D012394:Rosaniline Dyes; C005095:malachite green; D000251:Adenosine Triphosphatases; D017301:Ca(2+) Mg(2+)-ATPase; D001205:Ascorbic Acid
Mikromodifikatsiia opredeleniia ATFaznoĭ aktivnosti tkaneĭ i ikh biostruktur s ispol'zovaniem malakhitovogo zelenogo.