Site of binding of IgG2b and IgG2a by mouse macrophage Fc receptors by using cyanogen bromide fragments.
Cyanogen bromide fragments of murine IgG2b and IgG2a immunoglobulins were used to localize the sequences that are bound by specific IgG2b and IgG2a Fc receptors on murine macrophages. One fragment from the CH2 domain of IgG2b bound to the gamma 2b Fc receptor. Two fragments from IgG2a--one one from the CH2 domain, differing by only four amino acids from the homologous IgG2b fragment, and the other from the CH3 domain--specifically bound to the gamma 2a Fc receptor. In both a rosetting assay and a radioactive binding assay, these two fragments from IgG2a competed with intact IgG2a: however, they did not compete with each other. Rather, binding of the fragment from the CH3 domain of IgG2a augmented the binding of the fragment from the CH2 domain of IgG2a but not that of the homologous fragment from IgG2b. The binding of both IgG2a fragments was abolished by trypsin treatment of macrophages. These data suggest that 1) a sequence in the CH2 domain of IgG2b is sufficient for binding to the gamma 2b Fc receptor, 2) sequences from both the CH2 and CH3 domains of IgG2a bind to the gamma 2a Fc receptor, and 3) the binding of sequences from the CH3 domain of IgG2a may induce a conformational change in the gamma 2a Fc receptor that leads to enhanced binding of sequences from the CH2 domain.
Diamond
B
B
Boccumini
L
L
Birshtein
B K
BK
D000818:Animals; D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D003488:Cyanogen Bromide; D007128:Immunoglobulin Fragments / Q000276:immunology* / Q000302:isolation & purification; D007074:Immunoglobulin G / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D011961:Receptors, Fc / Q000032:analysis / Q000187:drug effects / Q000378:metabolism*; D017452:Receptors, IgG; D012397:Rosette Formation; D014357:Trypsin / Q000494:pharmacology
D007128:Immunoglobulin Fragments; D007074:Immunoglobulin G; D011961:Receptors, Fc; D017452:Receptors, IgG; D014357:Trypsin; D003488:Cyanogen Bromide