Plasminogen activator-anti-human fibrinogen conjugate.
A covalent conjugate between the plasminogen activator urokinase and polyclonal rabbit anti-human fibrinogen has been formed using the heterobifunctional coupling reagent N-succinimidyl 3-(2-pyridyldithio) propionate. The resultant urokinase-anti-human fibrinogen conjugate was separated from unreacted material by gel filtration. The conjugate exhibited amidase activity against the small chromogenic substrate pyroglutamyl-glycyl-arginine-p-nitroanilide as well as plasminogen activator activity in an assay employing plasminogen and the plasmin substrate D-valyl-leucyl-lysine-p-nitroanilide. Retention of antibody specificity for fibrinogen was demonstrated using an enzyme linked immunoassay procedure. The conjugate was found to have greater stability in human plasma than unconjugated urokinase.
Sevilla
C L
CL
Mahle
N H
NH
Boylan
C M
CM
Callewaert
D M
DM
D000906:Antibodies; D003432:Cross-Linking Reagents; D005340:Fibrinogen / Q000276:immunology*; D005341:Fibrinolysin / Q000378:metabolism; D006801:Humans; D013379:Substrate Specificity; D014568:Urokinase-Type Plasminogen Activator
D000906:Antibodies; D003432:Cross-Linking Reagents; D005340:Fibrinogen; D005341:Fibrinolysin; D014568:Urokinase-Type Plasminogen Activator