Localization of fucosyl moieties in the mouse renal cortex by lectin histochemistry using the fucose binding lectins LTA and UEA I and by autoradiography using 3H-labelled fucose.
The binding patterns of the two fucose binding lectins, Lotus tetragonolobus (LTA) and Ulex europeus I (UEA I) were investigated using fluorescence lectin histochemistry on the unfixed renal cortex of the mouse (NMRI) embedded in LR-Gold. The fluorescence staining results were compared with the autoradiographic localization of the incorporation of radioactive fucose into the renal cortex. For this study the turnover of incorporated 3H-fucose in the renal cortex was investigated 30 min, 2 h and 8 h after application. The localization of the radioactive fucose within the renal cortex corresponded well to the labelling pattern observed for lecting histochemistry using LTA. In contrast, with UEA I, no binding sites for this lectin could be observed. The results of our investigation clearly showed that fucosyl moieties in the renal cortex of the NMRI mouse are recognized by the fucose binding lecting LTA, but not by UEA I and that postembedding fluorescence histochemistry with LTA on the LR-Gold embedded kidney is a suitable technique for the localization of fucosyl moieties at the light microscopical level.
Herken
R
R
Zentrum Anatomie, Abteilung Histologie, Göttingen, Federal Republic of Germany.
Fussek
M
M
Zarfl
A
A
D000818:Animals; D001345:Autoradiography; D005260:Female; D005643:Fucose / Q000378:metabolism*; D006651:Histocytochemistry; D007672:Kidney Cortex / Q000378:metabolism* / Q000648:ultrastructure; D037102:Lectins / Q000378:metabolism*; D051379:Mice; D037121:Plant Lectins
D037102:Lectins; D037121:Plant Lectins; C036850:Ulex europaeus lectins; C027112:fucose-binding lectin; D005643:Fucose