title: Liquid chromatography with electrochemical detection for the determination of choline and acetylcholine in plasma and red blood cells. Failure to detect acetylcholine in blood of humans and mice.
abstract: An assay is described for the measurement of choline in plasma and red blood cells using liquid chromatography, an enzyme reactor and electrochemical detection after a simple sample pretreatment. The intra-assay coefficient of variation for choline was 6.2 and 3.8% in plasma and in red blood cells, respectively. Using this method we have re-investigated the presence of acetylcholine in blood constituents. We were not able to demonstrate acetylcholine with a limit of detection of 10 pmol per ml of plasma or per ml of red blood cells.
authors.affiliation: Department of Medicinal Chemistry, University of Groningen, The Netherlands.
authors.forename: G
authors.initials: G
authors.lastname: Damsma
authors.forename: F
authors.initials: F
authors.lastname: Flentge
mesh_terms: D000109:Acetylcholine / Q000097:blood*; D000818:Animals; D002794:Choline / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D004912:Erythrocytes / Q000032:analysis*; D006801:Humans; D051379:Mice; D009388:Neostigmine / Q000494:pharmacology; D011869:Radioligand Assay; D013045:Species Specificity
chemical_list: D009388:Neostigmine; D002794:Choline; D000109:Acetylcholine