title: Clonal nature of murine hemopoietic blast cell colonies.
abstract: Murine hemopoietic blast cell colonies obtained from spleen cells of 5-fluorouracil (5-FU)-treated mice give rise to many multilineage colonies including granulocyte - erythrocyte - macrophage - megakaryocyte (GEMM) colonies in secondary cultures. Progenitor cells for blast cell colonies are considered to be more primitive than colony forming units (CFU)-GEMM. To determine whether they are clonal, we examined the phosphoglycerate kinase-1 (PGK-1) isozyme type of colonies originally grown from spleen cells of 5-FU-treated mice which had PGK-1 isozyme mosaicism. PGK assays of whole secondary colonies derived from one blast cell colony showed that they were either of type A or type B but not both. These results suggest that murine hemopoietic blast cell colonies are clonal.
authors.affiliation: Second Department of Internal Medicine, Hyogo College of Medicine, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Fujimori
authors.forename: H
authors.initials: H
authors.lastname: Hara
authors.forename: T
authors.initials: T
authors.lastname: Taguchi
authors.forename: Y
authors.initials: Y
authors.lastname: Kitamura
authors.forename: K
authors.initials: K
authors.lastname: Nagai
mesh_terms: D000818:Animals; D002999:Clone Cells / Q000166:cytology*; D003114:Colony-Forming Units Assay; D005260:Female; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D051379:Mice; D009030:Mosaicism; D010735:Phosphoglycerate Kinase; D013154:Spleen / Q000166:cytology
chemical_list: D010735:Phosphoglycerate Kinase