title: Retinyl ester synthesis by isolated adult rabbit lung type II cells.
abstract: Type II alveolar cells were isolated from adult rabbit lungs and then cultured on monolayers for 16 hours. These cells were then covered with buffered medium containing [3H]-retinol. After 30-120 minutes incubation, the cells were extracted with Hexane: Ethanol and the hexane extract analyzed by HPLC. A linear synthesis of [3H]-retinyl palmitate with time of incubation was demonstrated.
authors.affiliation: University of Wisconsin Perinatal Center, Madison.
authors.forename: R D
authors.initials: RD
authors.lastname: Zachman
authors.forename: F H
authors.initials: FH
authors.lastname: Tsao
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004224:Diterpenes; D011650:Pulmonary Alveoli / Q000378:metabolism*; D011817:Rabbits; D000084562:Retinyl Esters; D014801:Vitamin A / Q000031:analogs & derivatives* / Q000096:biosynthesis
chemical_list: D004224:Diterpenes; D000084562:Retinyl Esters; D014801:Vitamin A; C014794:retinol palmitate