Poly-N-acetyllactosamines synthesized by cultured Ehrlich carcinoma cells: application of endo-beta-galactosidase C for analysis of the terminal structure.
Poly-N-acetyllactosamines were prepared from Ehrlich carcinoma cells cultured in the presence of [14C]galactose. Methylation analysis indicated that 31% of the galactose was in the non-reducing end. Of it, 77% was cleaved by alpha-galactosidase, and 56% was released as a disaccharide by endo-beta-galactosidase C. Methylation analysis confirmed that the released disaccharide was mostly Gal alpha 1----3Gal. Therefore, Gal alpha 1----3Gal structure, not Gal alpha 1----3(Gal alpha 1----6)Gal structure, was the major alpha-galactosyl structure in the poly-N-acetyllactosamines synthesized. Furthermore, alpha-galactosidase digestion did not change the content of disubstituted galactosyl residues. Thus, Gal alpha 1----3(Gal alpha 1----6)Gal structure, which was suggested to be the sole non-reducing terminal structure of poly-N-acetyllactosamines of Ehrlich carcinoma cells, was not detected in significant amounts under the present experimental conditions.
Kamada
Y
Y
Department of Biochemistry, Faculty of Medicine, Kagoshima University.
Muramatsu
H
H
Kawata
M
M
Takamizawa
H
H
Muramatsu
T
T
D000818:Animals; D002250:Carbon Radioisotopes; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism*; D002850:Chromatography, Gel; D002855:Chromatography, Thin Layer; D005696:Galactosidases / Q000378:metabolism*; D006026:Glycoside Hydrolases; D008745:Methylation; D011134:Polysaccharides / Q000032:analysis / Q000096:biosynthesis*; D001616:beta-Galactosidase / Q000378:metabolism*
D002250:Carbon Radioisotopes; D011134:Polysaccharides; C037199:poly-N-acetyllactosamine; D005696:Galactosidases; D006026:Glycoside Hydrolases; C057030:endo-beta-D-galactosidase C; D001616:beta-Galactosidase