Bioanalysis of (E)-5-(2-bromovinyl)-2'-deoxyuridine.
(E)-5-(2-Bromovinyl)-2'-deoxyuridine is an antiviral drug that is experimentally used for modulation of the antitumour effect of fluoropyrimidines, such as ftorafur and 5-fluorouracil. The isolation of the analyte, in the presence of 5-fluorouracil, from the matrix is performed either by means of a simple protein precipitation (plasma) or by means of a liquid-liquid extraction with ethyl acetate (urine). Following pretreatment, the analyte is analysed by reversed-phase chromatography and quantified by absorbance detection at 307 nm. The minimum detectable concentration in plasma and urine samples is ca. 6 ng/ml. The recovery after deproteination of plasma samples is 75%, while after liquid-liquid extraction of urine the recovery amounts 92%. The degree of protein binding of the analyte, measured by ultrafiltration, is found to be 97%. These data allow the bioanalysis of (E)-5-(2-bromovinyl)-2'-deoxyuridine for pharmacokinetic studies.
Reeuwijk
H J
HJ
Center for Bio-Pharmaceutical Sciences, Leiden University, The Netherlands.
Lingeman
H
H
Tjaden
U R
UR
de Bruijn
E A
EA
Keizer
H J
HJ
van der Greef
J
J
D000998:Antiviral Agents / Q000032:analysis* / Q000097:blood / Q000652:urine; D001798:Blood Proteins / Q000032:analysis; D001973:Bromodeoxyuridine / Q000031:analogs & derivatives* / Q000032:analysis / Q000097:blood / Q000652:urine; D002853:Chromatography, Liquid; D006801:Humans; D011485:Protein Binding
D000998:Antiviral Agents; D001798:Blood Proteins; C020235:brivudine; D001973:Bromodeoxyuridine