[Hybridization of A-factor deficient mutants of Streptomyces griseus by means of fusion of protoplasts].
Characteristics of 6 A-factor deficient mutants of S. griseus are presented. The common feature of the mutants was impairment of sporulation, formation of aerial mycelium and streptomycin synthesis. Pair-by-pair hybridization of the mutants was performed with protoplast fusion followed by regeneration. 9 pair couplings of the mutants were performed. In 3 of them sporulating recombinants were detected. The antibiotic production level in 70 hybrids was different and ranged from 0 to 1700 micrograms/ml. The morphological features of the colonies and the number of the spores formed were also different. The common feature of all the 70 sporulating hybrid strains was recovery of synthesis of A-factor, an endogenic regulator of S. griseus development. Therefore, in the A-factor deficient mutants impairment of A-factor synthesis was induced not by the plasmid elimination, as was suggested, but by mutation of separate genes.
Efremenkova
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OV
Anisova
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LN
Khokhlov
A S
AS
D015107:4-Butyrolactone / Q000031:analogs & derivatives; D006133:Growth Substances / Q000172:deficiency*; D006824:Hybridization, Genetic; D009154:Mutation; D011523:Protoplasts; D013305:Streptomyces griseus / Q000235:genetics*; D013307:Streptomycin / Q000096:biosynthesis
D006133:Growth Substances; C025035:A-factor (Streptomyces); D015107:4-Butyrolactone; D013307:Streptomycin
Gibridizatsiia A-faktornedostatochnykh mutantov Streptomyces griseus putem sliianiia protoplastov.