Functional expression of Aquaspirillum magnetotacticum genes in Escherichia coli K12.
Gene libraries from the magnetotactic bacterium, Aquaspirillum magnetotacticum were constructed in Escherichia coli with cosmids pLAFR3 and c2RB as vectors. Recombinant cosmids able to complement the thr-1, leuB, and proA mutations of the host were identified. The Pro+ recombinant cosmid restored wild-type phenotype in proA and proB but not in the proC mutants of E. coli. The results of restriction endonuclease digestion and Southern hybridization analysis indicate that the relevent leu and pro biosynthetic genes of A. magnetotacticum are not closely linked on the chromosome.
Waleh
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Molecular Biology Department, SRI International, Menlo Park, CA 94025.
D001419:Bacteria / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D003360:Cosmids; D004269:DNA, Bacterial; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D005822:Genetic Vectors; D011392:Proline / Q000378:metabolism; D014158:Transcription, Genetic
D004269:DNA, Bacterial; D011392:Proline