Generation of natural killer-like activity in mixed lymphocyte-tumor cell cultures. I. Role of HLA-DR antigens as stimulatory molecules.
A number of human lymphoid and non-lymphoid leukemic cell lines differing for expression of HLA-DR antigens were analyzed for the ability to induce natural killer(NK)-like activity and proliferation in lymphocytes from healthy donors. The ability to elicit the generation of NK-like activity in the responder lymphocytes varied greatly depending upon the type of antimitotic treatment (gamma-irradiation versus mitomycin C) received by the tumor cells prior to the start of the mixed cultures. By contrast, the induction of T-cell proliferation was positively correlated with the presence of DR molecules on the tumor cell lines. Nevertheless, DR- leukemic cells pretreated with the appropriate antimitotic agent did induce a proliferative response in the mixed cultures. T lymphocytes cultured without stimulator cells in spent medium containing high levels of cell-free DR antigens failed to undergo blastogenesis and proliferation, indicating that DR antigens can function as stimulatory molecules only when they are cell-associated.
Matera
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L
Francis
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Herlyn
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Trucco
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Santoli
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D002460:Cell Line; D002478:Cells, Cultured; D003602:Cytotoxicity, Immunologic; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D006801:Humans; D007694:Killer Cells, Natural / Q000166:cytology / Q000276:immunology* / Q000528:radiation effects; D007938:Leukemia / Q000276:immunology*; D004915:Leukemia, Erythroblastic, Acute / Q000276:immunology; D007951:Leukemia, Myeloid / Q000276:immunology; D015470:Leukemia, Myeloid, Acute / Q000276:immunology; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D016403:Lymphoma, Large B-Cell, Diffuse / Q000276:immunology
D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II