The removal of zinc from the mouse by polyamincarboxylic acids (CDTA and DTPA) following semichronic zinc ingestion.
Effects of ip treatment with diethylenetriaminepentaacetic acid (DTPA), and cyclohexanediaminetetraacetic acid (CDTA) on the zinc (Zn) excretion and Zn levels in selected mouse organs and tissues were assessed after mice were offered deionized water containing zinc acetate dihydrate (108 mg/kg/day) as the sole drinking fluid for 4 weeks. Following this period, the Zn-containing water was replaced by tap water and therapy with DTPA or CDTA was initiated. The animals received 6 injections of chelators or 0.9% saline (control group) on alternate days for 2 weeks of treatment. The dose of chelating agents was approximately equal to 1/4 of their respective ip LD50 values. Mice were housed in metabolic cages, and urine and feces were collected 24 hr after the first, fourth and sixth administration of the chelators. Six animals in each group were sacrificed at the same days. Although feces was the predominant route of elimination for Zn, only DTPA significantly increased the fecal excretion of Zn after the first administration of chelator. Treatment with DTPA or CDTA resulted in a significant decrease in the concentration of Zn in brain, spleen, and heart after the first injection. DTPA was consistently the most effective in increasing the urinary and fecal excretion of Zn and reducing the concentration of the metal found in various tissues. CDTA would be considered as a possible alternative.
Domingo
J L
JL
Laboratory of Toxicology and Biochemistry, School of Medicine, University of Barcelona, Spain.
Llobet
J M
JM
Colomina
M T
MT
Corbella
J
J
D000284:Administration, Oral; D000818:Animals; D002614:Chelating Agents / Q000627:therapeutic use*; D004492:Edetic Acid / Q000031:analogs & derivatives* / Q000627:therapeutic use; D005243:Feces / Q000032:analysis; D007928:Lethal Dose 50; D008297:Male; D051379:Mice; D004369:Pentetic Acid / Q000627:therapeutic use*; D014018:Tissue Distribution; D015032:Zinc / Q000633:toxicity* / Q000652:urine
D002614:Chelating Agents; C005390:CDTA; D004369:Pentetic Acid; D004492:Edetic Acid; D015032:Zinc