1,25-Dihydroxy-vitamin D in infant formulas.
A method is described for determining 1,25-dihydroxyvitamin D in infant formulas without using high pressure liquid chromatography to separate the vitamin D metabolites. After preparative chromatography with Silica Sep Pak and C18-Sep Pak cartridges the dihydroxylated vitamin D metabolite was quantified in a specific protein binding assay. The concentration of 1,25-dihydroxyvitamin D found was in a range between 2.5 and 11.3 pg/ml.
Kunz
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Institut für Physiologische Chemie, Universität Bonn, W. Germany.
D002117:Calcitriol / Q000032:analysis*; D006801:Humans; D007223:Infant; D007225:Infant Food / Q000032:analysis*
D002117:Calcitriol