title: Ethanol-induced alterations in exocrine pancreatic amino acid transport and secretion.
abstract: The effects of ethanol on exocrine pancreatic amino acid transport and secretion were investigated during perfusion of the isolated rat pancreas with ethanol concentrations ranging from 0.06% to 4.1%. Amino acid transport was quantitated using a rapid dual isotope dilution technique in which unidirectional substrate uptake (15-20 sec) is assessed relative to an extracellular tracer. Pancreatic secretion evoked by 0.3 microM carbachol was abolished during perfusion with 0.32% ethanol. Influx of L-lysine, L-serine and methylaminoisobutyric acid (MeAIB) was marginally increased by 0.32% ethanol but significantly inhibited during subsequent perfusion with 1.28-4.1% ethanol. Pancreatic oxygen consumption and effluent PCO2 levels decreased with increasing ethanol concentration, and the control venous pH (7.21 +/- 0.01, n = 8) gradually approached arterial pH values (7.46 +/- 0.02, n = 9). These results indicate that low concentrations of ethanol readily inhibit secretagogue-induced pancreatic secretion. Amino acid transport at the basolateral membrane of the exocrine pancreatic epithelium appears only to be inhibited after acute exposure to high ethanol concentrations.
authors.affiliation: Department of Physiology, King's College London, U.K.
authors.forename: G E
authors.initials: GE
authors.lastname: Mann
authors.forename: M
authors.initials: M
authors.lastname: Munoz
authors.forename: S
authors.initials: S
authors.lastname: Peran
mesh_terms: D000596:Amino Acids / Q000378:metabolism*; D000621:Aminoisobutyric Acids / Q000378:metabolism; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D004848:Epithelium / Q000378:metabolism; D000431:Ethanol / Q000494:pharmacology*; D008239:Lysine / Q000378:metabolism; D008297:Male; D010101:Oxygen Consumption / Q000187:drug effects; D010179:Pancreas / Q000378:metabolism*; D010477:Perfusion; D011312:Pressure; D051381:Rats; D011919:Rats, Inbred Strains; D012694:Serine / Q000378:metabolism
chemical_list: D000596:Amino Acids; D000621:Aminoisobutyric Acids; C017911:2-(methylamino)isobutyric acid; D000431:Ethanol; D012694:Serine; D008239:Lysine