title: Characterization of Yersinia enterocolitica, Y. intermedia, Y. aldovae, Y. frederiksenii, Y. kristensenii and Y. pseudotuberculosis by electrophoretic polymorphism of acid phosphatase, esterases, and glutamate and malate dehydrogenases.
abstract: Acid phosphatase, esterases, and glutamate and malate dehydrogenases of 192 strains of Yersinia enterocolitica, Y. intermedia, Y. aldovae, Y. frederiksenii, Y. kristensenii and Y. pseudotuberculosis were analysed by horizontal polyacrylamide agarose gel electrophoresis and by isoelectrofocusing in thin-layer polyacrylamide gels. The six species were clearly separated from each other by their distinct enzyme electrophoretic polymorphism. For Y. enterocolitica, the strains of biotype 5 were differentiated from the other biotypes by the mobility of glutamate dehydrogenase. For Y. frederiksenii, six zymotypes were delineated by pI and by the mobility of the enzymes. Variation in number or mobility of esterases within each species could represent a marker for epidemiological and ecological analyses. A linear relationship was obtained between the mean genetic diversity coefficient of enzymes and the mean percentage DNA-DNA relatedness of Y. intermedia, Y. aldovae, Y. enterocolitica and Y. frederiksenii.
authors.affiliation: Laboratoire de Microbiologie, Faculté de Médecine Xavier Bichat (Université Paris VII), France.
authors.forename: P
authors.initials: P
authors.lastname: Goullet
authors.forename: B
authors.initials: B
authors.lastname: Picard
mesh_terms: D000135:Acid Phosphatase / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D004950:Esterases / Q000032:analysis*; D005969:Glutamate Dehydrogenase / Q000032:analysis*; D007525:Isoelectric Focusing; D008291:Malate Dehydrogenase / Q000032:analysis*; D011110:Polymorphism, Genetic; D015007:Yersinia / Q000201:enzymology*; D015008:Yersinia enterocolitica / Q000201:enzymology; D015011:Yersinia pseudotuberculosis / Q000201:enzymology
chemical_list: D008291:Malate Dehydrogenase; D005969:Glutamate Dehydrogenase; D004950:Esterases; D000135:Acid Phosphatase