title: Fluid phase and mannose receptor-mediated uptake of horseradish peroxidase in mouse bone marrow-derived macrophages. Biochemical and ultrastructural study.
abstract: A detailed study of horseradish peroxidase (HRP) uptake by in vitro cultured bone marrow-derived macrophages was undertaken. Biochemical quantitations performed over a wide range of HRP concentrations, in the absence or presence of yeast mannan, showed that these macrophages pinocytose HRP by both fluid phase and mannose receptor-mediated uptake. The relative contribution of these two types of endocytosis varied with the concentration of enzyme in the extracellular medium. A morphological study at the light and electron microscope levels conducted in parallel confirmed the biochemical data.
authors.forename: T
authors.initials: T
authors.lastname: Lang
authors.forename: C
authors.initials: C
authors.lastname: de Chastellier
mesh_terms: D000818:Animals; D001693:Biological Transport, Active; D001853:Bone Marrow / Q000378:metabolism*; D002478:Cells, Cultured; D004705:Endocytosis; D006735:Horseradish Peroxidase / Q000378:metabolism*; D037181:Lectins, C-Type; D008264:Macrophages / Q000378:metabolism* / Q000648:ultrastructure; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins; D051379:Mice; D008854:Microscopy, Electron; D010544:Peroxidases / Q000378:metabolism*; D011956:Receptors, Cell Surface; D011971:Receptors, Immunologic / Q000378:metabolism*
chemical_list: D037181:Lectins, C-Type; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins; D011956:Receptors, Cell Surface; D011971:Receptors, Immunologic; D006735:Horseradish Peroxidase; D010544:Peroxidases