Sciatic nerve blood flow measured by laser Doppler flowmetry and [14C]iodoantipyrine.
Blood flow was examined in sciatic nerves of pentobarbital-anesthetized rats by means of laser Doppler flowmetry (LDF) and intravenous [14C]iodoantipyrine infusion. Continuous LDF signals demonstrated slow oscillations and acute, pressure-related changes in flow. The steady-state LDF signal was related linearly to nerve blood flow, as measured with [14C]iodoantipyrine, in intact nerves and nerves stripped of the epineurium. In 14 intact nerves, nerve blood flow averaged 0.27 +/- 0.03 (SE) ml X min-1 X g-1, whereas it averaged 0.13 +/- 0.01 in 5 stripped nerves. Autoradiographs of [3H]-nicotine-infused nerves and intra-arterial injection of 57Co-labeled microspheres demonstrated that flow was not uniform throughout the nerve cross section. The results indicate that LDF can be used to examine nerve blood flow in vivo, demonstrate a linear relation between the LDF signal and flow, and establish absolute values for blood flow in intact and stripped nerves of the anesthetized rat.
Rundquist
I
I
Smith
Q R
QR
Michel
M E
ME
Ask
P
P
Oberg
P A
PA
Rapoport
S I
SI
D000818:Animals; D000983:Antipyrine / Q000031:analogs & derivatives; D001345:Autoradiography; D001794:Blood Pressure; D002250:Carbon Radioisotopes; D003037:Cobalt Radioisotopes; D008297:Male; D008863:Microspheres; D009538:Nicotine; D051381:Rats; D012039:Regional Blood Flow; D012212:Rheology; D012584:Sciatic Nerve / Q000098:blood supply*; D014316:Tritium; D014463:Ultrasonography
D002250:Carbon Radioisotopes; D003037:Cobalt Radioisotopes; D014316:Tritium; D009538:Nicotine; D000983:Antipyrine; C008135:iodoantipyrine