Estrone- and dehydroepiandrosterone-sulfatase activities in human female epidermis.
Estrone (E1)-sulfatase and dehydroepiandrosterone (DHEA)-sulfatase activities were studied in human female epidermis. Skin specimens were obtained by abdominal or plantar biopsies. The apparent Michaelis-Menten constants for E1 and DHEA sulfatases were 35.2 microM and 8.7 microM, respectively. A substrate inhibition was only observed for DHEA sulfatase. Both sulfatases had an elevated temperature optimum (65 degrees C). The effect of inorganic salts was also tested. In normal epidermis, E1-sulfatase activity was constantly higher than DHEA-sulfatase activity, but no correlation between these activities was observed. On the other hand, E1- and DHEA-sulfatase activities were lower in plantar than in abdominal epidermis. In plantar epidermis of palmoplantar keratoderma, large variations in E1-sulfatase activity, but no significant variation in DHEA-sulfatase activity, were observed. In human epidermis, the findings were consistent with the existence of two different sulfatases: E1 sulfatase and DHEA sulfatase. It would also appear that sulfatase activities are not linked to the abnormal shedding of plantar stratum corneum in palmoplantar keratoderma.
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Nicollier
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Laurent
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Adessi
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D000005:Abdomen; D004485:Eczema / Q000201:enzymology; D004817:Epidermis / Q000201:enzymology*; D005260:Female; D006801:Humans; D007645:Keratoderma, Palmoplantar / Q000201:enzymology; D008593:Menopause; D043266:Steryl-Sulfatase; D013429:Sulfatases / Q000378:metabolism*; D013997:Time Factors
D013429:Sulfatases; C020121:estrone sulfatase; D043266:Steryl-Sulfatase