title: The phosphorylation of eukaryotic ribosomal protein S6 by protein kinase C.
abstract: Purified Ca2+-dependent and phospholipid-dependent protein kinase (protein kinase C) from bovine brain catalysed the phosphorylation of ribosomal protein S6 when incubated with 40S ribosomal subunits from rat liver or from hamster fibroblasts. The phosphorylation was dependent on Ca2+ and phospholipid, and occurred under ionic conditions similar to those which support protein biosynthesis in vitro. Protein kinase C phosphorylated at least three sites on ribosomal protein S6 when incubated with unphosphorylated ribosomes, and increased the extent of phosphorylation of ribosomes previously phosphorylated predominantly on two sites by cyclic-AMP-dependent protein kinase, converting some molecules to the tetraphosphorylated or pentaphosphorylated form. This indicates that protein kinase C can phosphorylate sites on ribosomal protein S6 other than those phosphorylated by the cyclic-AMP-dependent protein kinase, and this conclusion was confirmed by analysis of tryptic phosphopeptides. These results strengthen the possibility that protein kinase C might be involved in catalysing the multisite phosphorylation of ribosomal protein S6 in certain circumstances in vivo.
authors.forename: P J
authors.initials: PJ
authors.lastname: Parker
authors.forename: M
authors.initials: M
authors.lastname: Katan
authors.forename: M D
authors.initials: MD
authors.lastname: Waterfield
authors.forename: D P
authors.initials: DP
authors.lastname: Leader
mesh_terms: D000818:Animals; D002384:Catalysis; D006224:Cricetinae; D005347:Fibroblasts / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D010455:Peptides / Q000302:isolation & purification; D010766:Phosphorylation; D011493:Protein Kinase C; D011494:Protein Kinases / Q000378:metabolism*; D051381:Rats; D038601:Ribosomal Protein S6; D012269:Ribosomal Proteins / Q000378:metabolism*
chemical_list: D010455:Peptides; D038601:Ribosomal Protein S6; D012269:Ribosomal Proteins; D011494:Protein Kinases; D011493:Protein Kinase C