Co-operative effect of MCF and MAF(IFN-gamma) in the protection of mice against Listeria monocytogenes.
The effects of macrophage chemotactic factor (MCF) and macrophage-activating factor (MAF) on protection to Listeria monocytogenes were analysed using 'MCF-rich fraction' and murine recombinant interferon-gamma (rIFN-gamma). Recombinant IFN-gamma showed no macrophage chemotactic activity in the assays performed in vitro and in vivo. Although a single injection of either MCF-rich fraction or rIFN-gamma into the footpads of mice led to a significant degree of anti-listerial activity, the highest degree of protection was observed when injected together. The i.v. administration of 20,000 U rIFN-gamma did not raise significant protective activity against Listeria; however, injection with rIFN-gamma prior to that with the MCF-rich fraction into the footpad produced a higher level of protective activity than the group treated with MCF alone. In addition to MAF(IFN-gamma), MCF seems to play another important role in the full expression of protection against L. monocytogenes.
Handa
T
T
Department of Immunology, Kyushu University, Fukuoka, Japan.
Mitsuyama
M
M
Serushago
B A
BA
Muramori
K
K
Nomoto
K
K
D000818:Animals; D002630:Chemotactic Factors / Q000276:immunology*; D005260:Female; D007371:Interferon-gamma / Q000276:immunology*; D008088:Listeriosis / Q000517:prevention & control*; D008262:Macrophage Activation; D008264:Macrophages; D051379:Mice; D008807:Mice, Inbred BALB C; D011994:Recombinant Proteins
D002630:Chemotactic Factors; D011994:Recombinant Proteins; D007371:Interferon-gamma