title: Defective co-translational formation of disulphide bonds in protein disulphide-isomerase-deficient microsomes.
abstract: The formation of disulphide bonds in mammalian secretory and cell-surface proteins occurs in the lumen of the endoplasmic reticulum and is believed to be catalysed by the enzyme protein disulphide-isomerase (PDI). The evidence for this physiological role for PDI is circumstantial and relates to the cell and tissue distribution of the enzyme, its developmental behaviour and its catalytic properties in vitro. A clear requirement for PDI in the correct folding or assembly of disulphide-bonded proteins during biosynthesis has not been demonstrated. We have prepared dog pancreas microsomes which are deficient in soluble lumenal proteins, including PDI, but which are still able to translocate and process proteins synthesized in vitro. Using the formation of intramolecular disulphide bonds during the in vitro synthesis of gamma-gliadin, a wheat storage protein, as a model, we have demonstrated that these microsomes are defective in co-translational formation of disulphide bonds. Reconstitution of these microsomes with purified PDI reverses this defect.
authors.affiliation: Biological Laboratory, The University, Canterbury, Kent, UK.
authors.forename: N J
authors.initials: NJ
authors.lastname: Bulleid
authors.forename: R B
authors.initials: RB
authors.lastname: Freedman
mesh_terms: D000818:Animals; D004220:Disulfides / Q000096:biosynthesis*; D004285:Dogs; D005903:Gliadin / Q000096:biosynthesis*; D007535:Isomerases / Q000172:deficiency / Q000502:physiology*; D008861:Microsomes / Q000201:enzymology*; D010179:Pancreas / Q000201:enzymology; D010940:Plant Proteins / Q000096:biosynthesis*; D010957:Plasmids; D014176:Protein Biosynthesis; D019704:Protein Disulfide-Isomerases
chemical_list: D004220:Disulfides; D010940:Plant Proteins; D005903:Gliadin; D007535:Isomerases; D019704:Protein Disulfide-Isomerases