Differential requirements for the production by a T helper clone of the lymphokines involved in the induction of polyclonal proliferation and differentiation of unstimulated B lymphocytes.
Antigen-activated T helper (TH) cells secrete into their supernatant various lymphokines that are able to drive B cells to proliferate and to differentiate into Ig-secreting cells. In this report, we compared the production of these two types of activities by a TH clone. We found that B cell proliferating activity was released by TH cells under conditions in which T cell proliferation and the release of the B cell differentiating activity were totally blocked by anti-L3T4 monoclonal antibody GK1.5. The release of these two activities also dissociated during the reversion of T cells to the resting state after activation with antigen. Two weeks after activation, the T cell clone still secreted B cell proliferating activity, but did not secrete B cell differentiating activity. Three to four weeks after activation, neither activity was produced. The data suggest that the genes coding for these two activities are independently regulated in activated T cells. The implications of these observations concerning B lymphocyte development are discussed.
Gougeon
M L
ML
Leclercq
L
L
Bismuth
G
G
Thèze
J
J
D000818:Animals; D000944:Antigens, Differentiation, B-Lymphocyte; D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology*; D002454:Cell Differentiation; D002455:Cell Division; D006133:Growth Substances / Q000378:metabolism; D015847:Interleukin-4; D007518:Isoantibodies / Q000276:immunology; D007700:Kinetics; D008211:Lymphocyte Cooperation; D008222:Lymphokines / Q000378:metabolism; D008297:Male; D051379:Mice; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology* / Q000378:metabolism
D000944:Antigens, Differentiation, B-Lymphocyte; D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface; D006133:Growth Substances; D007518:Isoantibodies; D008222:Lymphokines; D015847:Interleukin-4