Inhibition of cell migrations by the linoleic acid oxygenation product 9S-hydroxy 10E,12Z octadecadienoic acid (9-HODE).
The linoleic acid metabolite 9S-HODE was prepared by means of tomato fruit lipoxygenase and purified by high-performance liquid chromatography (HPLC) to a high steric purity as judged by chiral-phase HPLC. 9S-HODE caused in the concentration range between 0.01 and 1 microM a strong dose-dependent inhibition of the migration of both cultured porcine aortic endothelial cells and of phytohaemagglutinin-stimulated human mononuclear cells. The effect was not observed with another polyenoic fatty acid metabolite, 15S-hydroperoxy-5Z,8Z,11Z,13E-eicosatetraenoic acid (15-HPETE). The results are discussed in the light of other biological actions of 9-HODE recently described.
Pankonin
G
G
Department of Pharmacy, Ernst-Moritz-Arndt University Greifswald, GDR.
Mahmood
S A
SA
Kuhn
H
H
Schewe
T
T
Pilgrim
H
H
Teuscher
E
E
D000818:Animals; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000502:physiology*; D006801:Humans; D008041:Linoleic Acids / Q000096:biosynthesis / Q000494:pharmacology*; D044243:Linoleic Acids, Conjugated; D008084:Lipoxygenase / Q000378:metabolism; D009000:Monocytes / Q000502:physiology*; D010944:Plants / Q000201:enzymology; D013552:Swine
D008041:Linoleic Acids; D044243:Linoleic Acids, Conjugated; C024347:9-hydroxy-10,12-octadecadienoic acid; D008084:Lipoxygenase