title: Isolation of labile Fcgamma-receptors from human peripheral blood lymphocytes and production of an antiserum.
abstract: In this study, we have isolated membranelabile Fcgamma-receptors (i.e. FcgammaR I) from normal human peripheral blood lymphocytes and have produced a rabbit antiserum to this protein. Using this antiserum, we have shown that membrane-labile and membrane-stable (i.e. FcgammaR II) Fcgamma-receptors are antigenically distinct and that these two forms of the receptors probably coexist on the same lymphocyte subpopulation. Moreover, it was apparent that lymphocyte FcgammaR Is are distinct from FcgammaRs expressed on other cell types (e.g. monocytes, polymorphs and spermatozoa). Preliminary evidence does suggest, however, that human platelets express an FcgammaR which is antigenically similar to human lymphocyte FcgammaR I.
authors.forename: G P
authors.initials: GP
authors.lastname: Sandilands
authors.forename: M G
authors.initials: MG
authors.lastname: Peel
authors.forename: K S
authors.initials: KS
authors.lastname: Froebel
authors.forename: J J
authors.initials: JJ
authors.lastname: Belch
authors.forename: R N
authors.initials: RN
authors.lastname: MacSween
mesh_terms: D000818:Animals; D000961:Antilymphocyte Serum / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008214:Lymphocytes / Q000276:immunology*; D008297:Male; D008970:Molecular Weight; D010974:Platelet Aggregation; D011817:Rabbits; D011961:Receptors, Fc / Q000276:immunology* / Q000302:isolation & purification; D017452:Receptors, IgG; D012397:Rosette Formation
chemical_list: D000961:Antilymphocyte Serum; D011961:Receptors, Fc; D017452:Receptors, IgG