title: Expression of tandem gene fusions in transgenic tobacco plants.
abstract: We have studied the expression of four sets of tandem gene fusions in transgenic tobacco plants. This was to determine if the problem of between-transformant variability in expression of introduced genes could be overcome by using a linked reference gene as a co-ordinately expressed control. Tandem gene fusions containing identical 5' flanking regions (SSU301-ocs with either SSU301-cat or SSU301-SSU911) were not co-ordinately expressed in the transgenic tobacco plants whereas the tandem gene fusions containing similar but not identical 5' flanking regions (SSU301-ocs with SSU911-cat or SSU911-SSU301) were co-ordinately expressed. The lack of co-ordinate expression of some of the tandem gene fusions appears to be partially explained by absence of the corresponding genomic DNA segments in the transgenic plants.
authors.affiliation: Advanced Genetic Sciences Inc, Oakland, CA 94608.
authors.forename: C
authors.initials: C
authors.lastname: Dean
authors.forename: M
authors.initials: M
authors.lastname: Favreau
authors.forename: S
authors.initials: S
authors.lastname: Tamaki
authors.forename: D
authors.initials: D
authors.lastname: Bond-Nutter
authors.forename: P
authors.initials: P
authors.lastname: Dunsmuir
authors.forename: J
authors.initials: J
authors.lastname: Bedbrook
mesh_terms: D005786:Gene Expression Regulation; D005818:Genetic Engineering; D005822:Genetic Vectors; D005810:Multigene Family; D010947:Plants, Toxic; D010957:Plasmids; D012231:Rhizobium / Q000235:genetics; D014026:Nicotiana / Q000235:genetics*; D014158:Transcription, Genetic