title: In vitro studies on the potential for pulpal cytotoxicity of glass-ionomer cements.
abstract: Elution samples of glass-ionomer cement were prepared in sterile tissue culture medium either by direct contact between the fluid and standard cement samples or through a layer of human dentin, and then tested for toxicity to cultured mouse fibroblasts (L929). The directly-prepared eluates of the cements were highly cytotoxic, but those prepared through dentin were of either limited or no cytotoxicity. The degree of toxicity of some directly-prepared eluates was reduced by adjustment of the pH to neutrality. It was apparent that dentin reduced the potential for cytotoxicity of glass-ionomer cements to a large degree. Proposed mechanisms for the reduction were limited availability of water at the dentin-cement interface and thus limited dissolution of components, buffering of acid components of the cements by dentin, or other chemical interactions with dentin.
authors.affiliation: Department of Dentistry, University of Adelaide, Australia.
authors.forename: W R
authors.initials: WR
authors.lastname: Hume
authors.forename: G J
authors.initials: GJ
authors.lastname: Mount
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D002478:Cells, Cultured; D003738:Dental Cements / Q000633:toxicity*; D003782:Dental Pulp / Q000166:cytology*; D003804:Dentin / Q000166:cytology*; D005260:Female; D005347:Fibroblasts / Q000187:drug effects*; D005899:Glass Ionomer Cements / Q000633:toxicity*; D006801:Humans; D008297:Male; D051379:Mice; D013936:Thymidine
chemical_list: D003738:Dental Cements; D005899:Glass Ionomer Cements; D013936:Thymidine