title: High-molecular-mass alkaline phosphatase: simplified and highly sensitive determination by liquid chromatography.
abstract: This simplified HPLC method for measurement of high-molecular-mass alkaline phosphatase (high-Mr AP; EC 3.1.3.1) in serum and bile is rapid (time for column preparation and separation 30 min), reproducible (CV 4.2%), and highly sensitive (detects high-Mr AP in healthy controls at 1-3% of total AP activity in serum), and is suitable for processing small batches of sample. We characterized high-Mr AP in serum and bile by incubating samples with L-phenylalanine, neuraminidase, 1-butanol, or wheat-germ lectin, and by determining stability to heat. High-Mr AP activity was determined in sera of patients with various liver diseases (4-32% of total AP serum activity) and results were compared with those by electrophoresis on agarose.
authors.affiliation: Klinik mit Poliklinik für Kinder und Jugendliche der Friedrich-Alexander Universität, Erlangen, F.R.G.
authors.forename: E
authors.initials: E
authors.lastname: Schoenau
authors.forename: W
authors.initials: W
authors.lastname: Boeswald
authors.forename: K H
authors.initials: KH
authors.lastname: Herzog
authors.forename: J
authors.initials: J
authors.lastname: Scheele
authors.forename: H J
authors.initials: HJ
authors.lastname: Boehles
authors.forename: K
authors.initials: K
authors.lastname: Stehr
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis* / Q000097:blood; D001646:Bile / Q000201:enzymology; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D007527:Isoenzymes / Q000032:analysis* / Q000097:blood; D008107:Liver Diseases / Q000201:enzymology; D008970:Molecular Weight
chemical_list: D007527:Isoenzymes; D000469:Alkaline Phosphatase