400-MHz 1H-NMR spectroscopy of fucosylated tetrasialyl oligosaccharides isolated from normal and cirrhotic alpha 1-acid glycoprotein.
The comparative study of fucosylated tetrasialyl-oligosaccharides isolated from normal and cirrhotic alpha 1-AGP was performed using permethylation and 400-MHz 1H-NMR spectroscopy. These results clearly show the tetraantennary structure of these two oligosaccharides with hyperfucosylation for the tetrasialylated fraction from cirrhotic alpha 1-AGP. In the latter oligosaccharide the simultaneous presence on two antennae (7 and 7') of the sialosyl Lewis X determinant NeuAc-(alpha 2-3) Gal(beta 1-4) [Fuc(alpha 1-3)] GlcNAc has been observed. Moreover the 5 and 5' antennae were alpha 2-6 sialylated but without fucose.
Wieruszeski
J M
JM
Laboratoire de Chimie Biologique et Unité Associée au CNRS no. 217, Université des Sciences et Techniques de Lille Flandres-Artois, Villeneuve d'Ascq, France.
Fournet
B
B
Konan
D
D
Biou
D
D
Durand
G
G
D001202:Ascitic Fluid / Q000032:analysis; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000032:analysis; D005643:Fucose / Q000032:analysis*; D006801:Humans; D008103:Liver Cirrhosis / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000032:analysis*; D009961:Orosomucoid / Q000032:analysis*; D012794:Sialic Acids / Q000032:analysis
D002241:Carbohydrates; D009844:Oligosaccharides; D009961:Orosomucoid; D012794:Sialic Acids; D005643:Fucose