Isolation of two forms of basement membrane proteoglycans.
Sequential extractions of the basement membrane producing Engelbreth-Holm-Swarm tumor yielded heparan sulfate proteoglycans with different size core proteins, but the same size heparan sulfate side chains. Saline, a nondenaturing solvent, extracted a small high density proteoglycan with a heterodisperse core protein of Mr = 95,000-130,000 whereas subsequent extraction with 7 M urea, a denaturing solvent, removed a large, low density proteoglycan with a Mr = 350,000-400,000 protein core. The denaturing conditions required for extraction of the large proteoglycan suggest that it interacts strongly with other basement membrane components. Antibodies to these proteoglycans cross-react with both proteoglycans, but the large proteoglycan has additional antigenic sites not present on the small proteoglycan. These proteoglycans may be derived from the same or similar gene products.
Hassell
J R
JR
Leyshon
W C
WC
Ledbetter
S R
SR
Tyree
B
B
Suzuki
S
S
Kato
M
M
Kimata
K
K
Kleinman
H K
HK
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001485:Basement Membrane / Q000032:analysis*; D002499:Centrifugation, Density Gradient; D011508:Chondroitin Sulfate Proteoglycans / Q000302:isolation & purification*; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D006025:Glycosaminoglycans / Q000302:isolation & purification*; D019812:Heparan Sulfate Proteoglycans; D006497:Heparan Sulfate / Q000302:isolation & purification*; D007106:Immune Sera; D051379:Mice; D008810:Mice, Inbred C57BL; D008970:Molecular Weight; D009374:Neoplasms, Experimental / Q000032:analysis* / Q000648:ultrastructure; D011133:Polysaccharide-Lyases / Q000378:metabolism; D011509:Proteoglycans / Q000302:isolation & purification*; D013431:Sulfates / Q000378:metabolism
D000596:Amino Acids; D011508:Chondroitin Sulfate Proteoglycans; D006025:Glycosaminoglycans; D019812:Heparan Sulfate Proteoglycans; D006497:Heparan Sulfate; D007106:Immune Sera; D011133:Polysaccharide-Lyases; D011509:Proteoglycans; D013431:Sulfates; C021349:heparitinsulfate lyase