title: Xenobiotic oxidation by cytochrome P-450-enriched extracts of Streptomyces griseus.
abstract: Crude extracts of Streptomyces griseus grown on soybean flour-enriched medium contain high levels of cytochrome P-450. The cytochrome P-450-enriched fractions, obtained by ammonium sulfate fractionation (30-50% saturation), catalyze the NADPH-dependent oxidation of a variety of xenobiotics when complemented with both spinach ferredoxin:NADP+ oxidoreductase and spinach ferredoxin. Reactions observed are aromatic, benzylic and alicyclic hydroxylations, O-dealkylation, non-aromatic double bond epoxidation, N-oxidation and N-acetylation.
authors.affiliation: E.I. Du Pont de Nemours & Co. (Inc.), Central Research and Development Department, Wilmington, DE 19880-0228.
authors.forename: M K
authors.initials: MK
authors.lastname: Trower
authors.forename: F S
authors.initials: FS
authors.lastname: Sariaslani
authors.forename: F G
authors.initials: FG
authors.lastname: Kitson
mesh_terms: D000645:Ammonium Sulfate; D000814:Aniline Compounds / Q000378:metabolism; D001554:Benzene / Q000378:metabolism; D001578:Benzopyrans / Q000378:metabolism; D055598:Chemical Phenomena; D002621:Chemistry; D003374:Coumarins / Q000378:metabolism; D003510:Cyclohexanes / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000302:isolation & purification / Q000378:metabolism*; D004958:Estradiol / Q000378:metabolism; D005287:Ferredoxin-NADP Reductase / Q000378:metabolism; D005288:Ferredoxins / Q000378:metabolism; D005590:Fractional Precipitation; D007700:Kinetics; D009249:NADP / Q000494:pharmacology; D010084:Oxidation-Reduction; D011725:Pyridines / Q000378:metabolism; D013305:Streptomyces griseus / Q000201:enzymology*; D013379:Substrate Specificity; D014050:Toluene / Q000378:metabolism; D015262:Xenobiotics / Q000378:metabolism*
chemical_list: D000814:Aniline Compounds; D001578:Benzopyrans; D003374:Coumarins; D003510:Cyclohexanes; D005288:Ferredoxins; D011725:Pyridines; D015262:Xenobiotics; C014163:precocene II; C017299:7-ethoxycoumarin; D014050:Toluene; D004958:Estradiol; D009249:NADP; D003577:Cytochrome P-450 Enzyme System; D005287:Ferredoxin-NADP Reductase; D001554:Benzene; C023666:pyridine; C023650:aniline; D000645:Ammonium Sulfate