[Contribution of nuclear magnetic resonance spectroscopy of phosphorus-31 and carbon-13 in the study of myocardial metabolism].
The use of NMR spectroscopy in the non-invasive assessment of myocardial metabolism has greatly increased over the last decade. The initial experiments were performed on isolated perfused heart preparations, but these have since been extended to whole animal and clinical studies. The use of the phosphorus-31 nucleus allows assessment of energetic metabolism and intramyocardial pH. Carbon-13 spectroscopy based on the use of substrates selectively enriched with the C-13 isotope enables the study of a specific chosen metabolic pathway and provides qualitative and quantitative information about the metabolic changes. Research is now preceding in two directions: firstly, the study of fundamental problems such as the mechanisms of ischaemia, the consequences of intracellular acidosis and the precise role of ATP and phosphocreatine: secondly, very active clinical and pharmacological research in using NMR in animal models of cardiac pathology. Finally, recent technological progress suggests that NMR spectroscopy will soon be used for direct studies of the human heart.
Bernard
M
M
UA CNRS 1186, faculté de médecine de la Timone, Marseille.
Cozzone
P J
PJ
D002250:Carbon Radioisotopes; D002314:Cardioplegic Solutions / Q000494:pharmacology; D002952:Citric Acid Cycle; D003327:Coronary Disease / Q000378:metabolism / Q000503:physiopathology; D004734:Energy Metabolism; D006003:Glycogen / Q000378:metabolism; D006324:Heart Arrest, Induced; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D009206:Myocardium / Q000201:enzymology / Q000378:metabolism*; D010761:Phosphorus Radioisotopes
D002250:Carbon Radioisotopes; D002314:Cardioplegic Solutions; D010761:Phosphorus Radioisotopes; D006003:Glycogen
Apports de la spectroscopie de résonance magnétique nucléaire du phosphore-31 et du carbone-13 à l'étude du métabolisme myocardique.