Effect of phorbolmyristate acetate on fatty acid uptake and distribution in human promyelocytic leukemia (HL-60) cells in vitro.
Human promyelocytic leukemia (HL-60) cells can be induced to differentiate to macrophages in vitro by phorbolmyristate acetate (PMA). HL-60 cells, unlike normal cells incorporated a major portion of linoleic acid (LA) and arachidonic acid (AA) in the ether lipid fraction. On exposure to PMA, similar to the normal cells tested, the fatty acids were incorporated mainly in the phospholipid fraction. Since, ether lipid pool is metabolically inert and considered as a storage pool where as the phospholipid fraction is a metabolically active pool this may explain, at least in part, the low metabolic rate of AA and the low phospholipase A2 activity in HL-60 cells.
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Department of Medicine, Nizam's Institute of Medical Sciences, Punjagutta, Hyderabad, India.
D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism; D001692:Biological Transport / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D005227:Fatty Acids / Q000378:metabolism*; D006410:Hematopoiesis / Q000187:drug effects; D006801:Humans; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000378:metabolism; D050356:Lipid Metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D001095:Arachidonic Acids; D005227:Fatty Acids; D008041:Linoleic Acids; D016718:Arachidonic Acid; D019787:Linoleic Acid; D013755:Tetradecanoylphorbol Acetate