Effect of different APO A-I containing lipoprotein particles on reverse cholesterol transport in fat cells.
Cholesterol efflux was studied in cultured mouse adipose cells after preloading with LDL cholesterol. Long term exposure to LpA-I particles isolated from HDL2 and HDL3 promoted cholesterol efflux while LpA-I:A-II particles isolated from both fractions did not. However, LpA-I and LpA-I:A-II particles isolated from both HDL subfractions were effective in competing for the binding of 125I-HDL3 to apo A-I/A-II cell surface receptor sites. These results underline the role of LpA-I particles in the promotion of cholesterol efflux and suggest that the concentrations and/or the relative proportions of LpA-I and LpA-I:A-II particles might be critical for the regulation of cholesterol efflux from adipose cells.
Barkia
A
A
SERLIA, Institut Pasteur, Lille, France.
Barbaras
R
R
Ghalim
N
N
Puchois
P
P
Ailhaud
G
G
Fruchart
J C
JC
D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D016632:Apolipoprotein A-I; D001054:Apolipoproteins A / Q000097:blood* / Q000494:pharmacology; D002460:Cell Line; D002784:Cholesterol / Q000378:metabolism*; D002999:Clone Cells; D006801:Humans; D008075:Lipoproteins, HDL / Q000097:blood* / Q000494:pharmacology; D051379:Mice
D016632:Apolipoprotein A-I; D001054:Apolipoproteins A; D008075:Lipoproteins, HDL; D002784:Cholesterol