The GTP-binding peptide of beta-tubulin. Localization by direct photoaffinity labeling and comparison with nucleotide-binding proteins.
The binding site of the guanine moiety of GTP on beta-tubulin was located within the peptide consisting of residues 63-77, AILVDLEPGTMDSVR. The result was obtained using direct photoaffinity labeling, peptide sequencing, and limited proteolysis. Peptides were identified by end-labeling with a monoclonal antibody against beta-tubulin whose epitope was located between 3 and 4 kDa from the C terminus. The sequence of the GTP-binding site is consistent with predictions from other GTP-binding proteins such as elongation factor Tu or ras p21.
Linse
K
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Max-Planck-Unit for Structural Molecular Biology, DESY, Hamburg, Federal Republic of Germany.
Mandelkow
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EM
D000345:Affinity Labels; D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D001921:Brain / Q000378:metabolism; D002918:Chymotrypsin; D004591:Electrophoresis, Polyacrylamide Gel; D006160:Guanosine Triphosphate / Q000378:metabolism*; D015151:Immunoblotting; D010446:Peptide Fragments / Q000378:metabolism; D010761:Phosphorus Radioisotopes; D011485:Protein Binding; D013552:Swine; D014404:Tubulin / Q000302:isolation & purification / Q000378:metabolism*
D000345:Affinity Labels; D010446:Peptide Fragments; D010761:Phosphorus Radioisotopes; D014404:Tubulin; D006160:Guanosine Triphosphate; D002918:Chymotrypsin