title: Lymphokine production by human peripheral blood lymphocytes: analysis by in situ hybridization.
abstract: Lymphokine production in human peripheral blood mononuclear cells (PBMNCs) was investigated by in situ hybridization with 35S-labelled RNA probes coding for interleukin 2 (IL-2), gamma-interferon (gamma-IFN) and granulocyte/macrophage colony stimulating factor (GM-CSF). The proportion of PBMNCs expressing lymphokine mRNA varied according to the stimuli used and followed similar patterns to those previously observed by Northern blot analysis. Only a relatively low proportion of PBMNCs expressed lymphokine mRNA after stimulation for a given time, and this seems not to be due to the heterogeneity of T cells.
authors.affiliation: Institute of Clinical Immunology, Inselspital, University of Bern, Switzerland.
authors.forename: G
authors.initials: G
authors.lastname: Qiu
authors.forename: J F
authors.initials: JF
authors.lastname: Gauchat
authors.forename: U
authors.initials: U
authors.lastname: Wirthmüller
authors.forename: A L
authors.initials: AL
authors.lastname: De Weck
authors.forename: B M
authors.initials: BM
authors.lastname: Stadler
mesh_terms: D003115:Colony-Stimulating Factors / Q000096:biosynthesis / Q000235:genetics; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D006133:Growth Substances / Q000096:biosynthesis / Q000235:genetics; D006801:Humans; D066298:In Vitro Techniques; D007371:Interferon-gamma / Q000096:biosynthesis / Q000235:genetics; D007376:Interleukin-2 / Q000096:biosynthesis / Q000235:genetics; D008214:Lymphocytes / Q000276:immunology*; D008222:Lymphokines / Q000096:biosynthesis* / Q000235:genetics; D009693:Nucleic Acid Hybridization; D015347:RNA Probes; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D003115:Colony-Stimulating Factors; D006133:Growth Substances; D007376:Interleukin-2; D008222:Lymphokines; D015347:RNA Probes; D012333:RNA, Messenger; D007371:Interferon-gamma; D016178:Granulocyte-Macrophage Colony-Stimulating Factor