title: Limitation of maximal O2 uptake and performance by acute hypoxia in dog muscle in situ.
abstract: The factors that determine maximal O2 uptake (VO2max) and muscle performance during severe, acute hypoxemia were studied in isolated, in situ dog gastrocnemius muscle. Our hypothesis that VO2max is limited by O2 diffusion in muscle predicts that decreases in VO2max, caused by hypoxemia, will be accompanied by proportional decreases in muscle effluent venous PO2 (PvO2). By altering the fraction of inspired O2, four levels of arterial PO2 (PaO2) [21 +/- 2, 28 +/- 1, 44 +/- 1, and 80 +/- 2 (SE) Torr] were induced in each of eight dogs. Muscle arterial and venous circulation was isolated and arterial pressure held constant by pump perfusion. Each muscle worked maximally (3 min at 5-6 Hz, isometric twitches) at each PaO2. Arterial and venous samples were taken to measure lactate, [H+], PO2, PCO2, and muscle VO2. Muscle biopsies were taken to measure [H+] (homogenate method) and lactate. VO2max decreased with PaO2 and was linearly (R = 0.99) related to both PVO2 and O2 delivery. As PaO2 fell, fatigue increased while muscle lactate and [H+] increased. Lactate release from the muscle did not change with PaO2. This suggests a barrier to lactate efflux from muscle and a possible cause of the greater fatigue seen in hypoxemia. The gas exchange data are consistent with the hypothesis that VO2max is limited by peripheral tissue diffusion of O2.
authors.affiliation: Department of Medicine, University of California, San Diego, La Jolla 92093.
authors.forename: M C
authors.initials: MC
authors.lastname: Hogan
authors.forename: J
authors.initials: J
authors.lastname: Roca
authors.forename: P D
authors.initials: PD
authors.lastname: Wagner
authors.forename: J B
authors.initials: JB
authors.lastname: West
mesh_terms: D000136:Acid-Base Equilibrium; D000818:Animals; D004285:Dogs; D005260:Female; D006614:Hindlimb; D006863:Hydrogen-Ion Concentration; D000860:Hypoxia / Q000097:blood / Q000378:metabolism*; D007773:Lactates / Q000378:metabolism; D019344:Lactic Acid; D008297:Male; D009132:Muscles / Q000378:metabolism*; D010100:Oxygen / Q000097:blood; D010101:Oxygen Consumption
chemical_list: D007773:Lactates; D019344:Lactic Acid; D010100:Oxygen