High-performance liquid chromatographic method for determination of ciprofloxacin in biological fluids.
A simple and precise high-performance liquid chromatographic procedure has been developed for the determination in biological fluids of ciprofloxacin, a new, with extended antibacterial spectrum, quinoline carboxylic acid. The work-up procedure involves a chemical extraction step followed by isocratic chromatography on a reversed-phase analytical column, with ultraviolet detection. The detection limit for blood levels is 10 ng/ml. The calibration curve is linear from this detection limit to 10 microgram/ml. The statistical analysis of the correlation made between this assay and an agar diffusion procedure during a pharmacokinetic study suggests the existence of one or more active metabolites which could be mainly excreted in the bile.
Jehl
F
F
Gallion
C
C
Debs
J
J
Brogard
J M
JM
Monteil
H
H
Minck
R
R
D000890:Anti-Infective Agents / Q000032:analysis* / Q000097:blood / Q000652:urine; D001646:Bile / Q000032:analysis; D001826:Body Fluids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D002939:Ciprofloxacin; D006801:Humans; D011804:Quinolines / Q000032:analysis* / Q000097:blood / Q000652:urine
D000890:Anti-Infective Agents; D011804:Quinolines; D002939:Ciprofloxacin