[Thyroid gland inositol-1-phosphate synthase (its purification and characteristics)].
Pig thyroid myoinositol-phosphate synthase was purified about 30 times using ammonium sulphate fractionation and DEAE cellulose chromatography. The enzyme preparation showed the activity of more than 70 mU/mg of protein. A partially purified synthase is a very labile enzyme. Its activity showed optimum value at pH 7.0. This activity appeared to be controlled by NH4+, Na+, and Li+ ions. The biological role of thyroid synthase has been discussed.
Stelmach
H
H
Zakładu Chemii Fizycznej, Instytutu Chemii AM w Białymstoku.
Jaroszewicz
L
L
D000818:Animals; D002852:Chromatography, Ion Exchange / Q000379:methods; D003470:Culture Media; D004789:Enzyme Activation / Q000502:physiology; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007296:Myo-Inositol-1-Phosphate Synthase / Q000302:isolation & purification* / Q000502:physiology; D013552:Swine; D013961:Thyroid Gland / Q000201:enzymology*
D003470:Culture Media; D007296:Myo-Inositol-1-Phosphate Synthase
Syntaza mio-inozytolo-1-fosforanowa z tarczycy (oczyszczanie i charakterystyka).