An ELISA for PGE2 utilizing monoclonal antibody.
An ELISA for PGE2 has been developed which is sensitive to concentrations of 0.5 to 20.0 ng PGE2/ml. Mouse monoclonal anti-PGE2 ascites is utilized in a binding competition between the test sample and an adsorbed conjugate of PGE2-BSA. The antibody which remains bound to the solid phase is quantitated colorimetrically by incubation with alkaline phosphatase-conjugated goat anti-mouse IgG followed by incubation with p-nitro-phenylphosphate. PGE1, PGA1, PGA2, PGB2, 6-keto-PGF1 alpha, PGF2 alpha, 13,14-dihydro-15-keto-PGE2, thromboxane B2 and arachidonic acid showed minimal cross-reactivity with the anti-PGE2. The PGE2 ELISA permits the quantitative analysis of large numbers of samples at a fraction of the cost and time required to process a commercial RIA kit. When linked to the appropriate computer software, data collection and analysis can be performed in less than 10 minutes per 96-well plate. Furthermore, the use of an ELISA system eliminates the radioactive and toxic chemical waste generated by RIA methods.
Neuman
R G
RG
Ayerst Laboratories Research, Inc., Department of Immunology, Princeton, NJ 08543.
Korn
J H
JH
Lally
E T
ET
Wood
D D
DD
Kimball
E S
ES
D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D003429:Cross Reactions; D003470:Culture Media / Q000032:analysis; D015232:Dinoprostone; D004797:Enzyme-Linked Immunosorbent Assay; D011458:Prostaglandins E / Q000032:analysis* / Q000276:immunology; D011459:Prostaglandins E, Synthetic / Q000032:analysis* / Q000276:immunology; D012997:Solvents; D013223:Statistics as Topic; D013582:Synovial Fluid / Q000166:cytology; D014556:Urine / Q000032:analysis
D000911:Antibodies, Monoclonal; D003470:Culture Media; D011458:Prostaglandins E; D011459:Prostaglandins E, Synthetic; D012997:Solvents; D015232:Dinoprostone