Identification of the major metabolite of 4,4'-thio-bis-(6-t-butyl-m-cresol).
Metabolism of 4,4'-thio-bis-(2-t-butyl-5-methylphenol)(TBBC) in rats resulted in the formation of a glucuronide conjugate of TBBC. This conjugate was identified by a combination of high-performance liquid chromatography (HPLC) and mass spectrometry and a tandem mass spectrometric method employing a fast particle ionization technique. A comparison of mass spectral data from the in-vivo metabolite of TBBC and an enzymically synthesized glucuronide conjugate of TBBC showed the metabolite to be the monoglucuronide.
Smith
R W
RW
Matthews
H B
HB
Parker
C E
CE
Hass
J R
JR
D000818:Animals; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D003408:Cresols / Q000378:metabolism*; D005965:Glucuronates / Q000378:metabolism; D008297:Male; D013058:Mass Spectrometry; D051381:Rats; D011916:Rats, Inbred F344
D003408:Cresols; D005965:Glucuronates; C040020:4,4'-thiobis(6-tert-butyl-3-cresol)