Role of a mouse monoclonal IgE antibody in passive transfer of immunity to Schistosoma japonicum infection.
We have been able to produce a mouse monoclonal IgE antibody specific to an adult worm antigen extracted from Schistosoma japonicum (Sj). The antibody was able to elicit passive cutaneous anaphylaxis in the rat skin against Sj with the highest titer of 1:256,000 but did not cross-react with S. mansoni antigen. The antibody recognized a 97-kDa molecule expressed on the surface of mechanically transformed schistosoma of S. japonicum. Passive transfer of the antibody into mice in the early stage of challenge infection resulted in a partial but significant reduction of recovery of adult worms. Induction of eosinophilia by an oral administration of embryonated eggs of Toxocara canis prior to challenge infection enhanced the reduction.
Kojima
S
S
Department of Parasitology, Chiba University School of Medicine, Japan.
Janecharut
T
T
Hata
H
H
Niimura
M
M
D000818:Animals; D000909:Antibodies, Helminth / Q000008:administration & dosage / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000008:administration & dosage / Q000276:immunology*; D004802:Eosinophilia / Q000150:complications; D007116:Immunization, Passive; D007073:Immunoglobulin E / Q000008:administration & dosage / Q000276:immunology*; D051379:Mice; D010323:Passive Cutaneous Anaphylaxis; D051381:Rats; D012549:Schistosoma japonicum / Q000276:immunology*; D012554:Schistosomiasis japonica / Q000517:prevention & control*; D014120:Toxocariasis / Q000150:complications
D000909:Antibodies, Helminth; D000911:Antibodies, Monoclonal; D007073:Immunoglobulin E