title: Presence of antibodies to human immunodeficiency virus-1 in reference reagents of human origin in diagnostic kits.
abstract: We tested 54 reagents of human origin that were included in a number of diagnostic kits to be used as positive or negative control material, and for quality assurance, for the presence of antibodies to human immunodeficiency virus-1 (HIV-1). Of these, 12 (22%) reagents were found to give positive results for antibodies to HIV-1 by enzyme-linked immunosorbent assay and/or supplementary tests. These results suggest that some diagnostic reagents of human origin may be contaminated with HIV-1 and potentially may be infectious.
authors.affiliation: National Biological Standards Laboratory, Commonwealth Department of Community Services and Health, Parkville, Vic.
authors.forename: F A
authors.initials: FA
authors.lastname: Brake
authors.forename: W J
authors.initials: WJ
authors.lastname: Maskill
authors.forename: T S
authors.initials: TS
authors.lastname: Howard
authors.forename: A M
authors.initials: AM
authors.lastname: Breschkin
authors.forename: S S
authors.initials: SS
authors.lastname: Gandhi
mesh_terms: D000914:Antibodies, Viral / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D004866:Equipment Contamination; D005189:False Positive Reactions; D006678:HIV / Q000276:immunology*; D015483:HIV Antibodies; D006801:Humans; D011786:Quality Control; D011933:Reagent Kits, Diagnostic
chemical_list: D000914:Antibodies, Viral; D015483:HIV Antibodies; D011933:Reagent Kits, Diagnostic