Modification of porcine zona pellucida with fluorescein isothiocyanate: evidence for the presence of a structural unit consisting of glycoproteins in the mammalian egg coat.
Intact porcine ova and mechanically isolated zonae pellucidae from the ova were treated with a limiting amount of FITC in isotonic solutions at different pHs. The modified zona proteins were fractionated into three families (FTC-PZP1-3) by HPLC on a gel filtration column. It was found by this HPLC that the amino groups of PZP3 hardly reacted with FITC, whereas those of PZP1 and 2 fairly reacted, reflecting the organization of these families in the zona structure. The difference in reactivity of the three families with FITC suggested the presence of a structural unit in the zona, thus supporting the filamentous model of Wassarman.
Hatanaka
Y
Y
Department of Chemistry, Faculty of Science, Chiba University, Japan.
Nakano
M
M
Tobita
T
T
D000818:Animals; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D005260:Female; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D006023:Glycoproteins / Q000302:isolation & purification*; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D010063:Ovum / Q000032:analysis* / Q000378:metabolism; D012995:Solubility; D013329:Structure-Activity Relationship; D013552:Swine; D013861:Thiocyanates; D014357:Trypsin; D015044:Zona Pellucida / Q000032:analysis*
D005452:Fluoresceins; D006023:Glycoproteins; D013861:Thiocyanates; D014357:Trypsin; D016650:Fluorescein-5-isothiocyanate