On the activities of parathyroid hormone-like factor and transforming growth factors in extract of pancreatic cancer associated with humoral hypercalcemia of malignancy.
Extract of exocrine pancreatic cancer associated with humoral hypercalcemia of malignancy was examined for biological activities of PTH-like factor and transforming growth factor (TGFA and TGFB), both of which are possible causes of hypercalcemia. The crude extract had both PTH-like and TGF activities. On Bio Gel P-60 column chromatography, PTH-like and TGFA activities were eluted at around 10 kD, whereas TGFB activity was eluted at around void fractions, 10 kD and 6 kD. Liver extract, used as a control material, exhibited only TGFB activity at around 6 kD. CM-cellulose column chromatography of 10 kD fractions resulted in a subtle distinction between PTH-like activity and TGF activities. Further fractionation of the peak with PTH-like activity on reverse-phase high performance liquid chromatography separated PTH-like activity distinctly from TGFB activity. TGFA activity was lost through the procedure. It was concluded that the exocrine pancreatic cancer associated with hypercalcemia produced not only PTH-like activity but also TGFA and TGFB activities. Several chromatographic analyses suggested that PTH-like activity and at least TGFB activity stem from distinct molecules and that the PTH-like factor has no significant TGFB activity intrinsically.
Akatsu
T
T
Third Department of Internal Medicine, National Defense Medical College, Saitama, Japan.
Nagata
N
N
Kugai
N
N
Yasutomo
Y
Y
Kinoshita
T
T
Kosano
H
H
Takatani
O
O
Takishima
K
K
Mamiya
G
G
D002460:Cell Line; D002850:Chromatography, Gel; D005260:Female; D006801:Humans; D006934:Hypercalcemia / Q000378:metabolism*; D008875:Middle Aged; D010190:Pancreatic Neoplasms / Q000378:metabolism*; D010281:Parathyroid Hormone / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism*; D011869:Radioligand Assay; D015291:Transforming Growth Factors; D014410:Tumor Stem Cell Assay
D010281:Parathyroid Hormone; D010455:Peptides; D015291:Transforming Growth Factors