Distribution of grifolan NMF-5N (I/B), a chemically modified antitumor beta-glucan in mice.
The distribution of a 3H-labeled chemically modified antitumor beta-glucan, grifolan NMF-5N (I/B), in various tissues after an injection into mice was examined in order to obtain information on distribution of the parent antitumor beta-glucan, grifolan NMF-5N. Grifolan NMF-5N was treated with sodium metaperiodate and sodium borotritide to obtain tritium-labeled grifolan NMF-5N [3H-grifolan (I/B)]. When 3H-grifolan (I/B) was administered into normal mice by intraperitoneal (i.p.) or intravenous (i.v.) injection, radioactivity was detected in various mouse tissues. Next, 3H-grifolan (I/B) was injected into tumor-bearing mice 7 d after the tumor inoculation, which is the most effective administration timing for the antitumor effect of grifolan NMF-5N. The results indicated a strong radioactivity in spleens and tumor masses. These results suggested a close relationship between the antitumor activity and the distribution of grifolan NMF-5N in mice.
Takeyama
T
T
Tokyo College of Pharmacy, Japan.
Suzuki
I
I
Ohno
N
N
Oikawa
S
S
Sato
K
K
Ohsawa
M
M
Yadomae
T
T
D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000493:pharmacokinetics* / Q000494:pharmacology; D002277:Carcinoma / Q000188:drug therapy; D005354:Fibrosarcoma / Q000188:drug therapy; D005936:Glucans / Q000493:pharmacokinetics* / Q000494:pharmacology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008813:Mice, Inbred ICR; D014018:Tissue Distribution; D047071:beta-Glucans
D000903:Antibiotics, Antineoplastic; D005936:Glucans; D047071:beta-Glucans; C048444:grifolan; C033363:beta-1,3-glucan