[Response to antitumor agents of human transplantable glioma implanted into chorioallantoic membrane of chick embryo].
In case of chemotherapy against brain tumors, it is most important to choose suitable drugs for brain tumors, since human tumors have different drug sensitivity and growth. Heretofore, the nitrosourea-induced rat glioma cell, such as C6, or immunodeficient mice were usually used for predicting the drug sensitivity of brain tumors. We took notice of Murphy's system for the chemosensitivity test, in which a human tumor is transplanted into the chorioallantoic membrane (CAM) of a chick embryo. By modifying the conventional Murphy's system, we studied the efficiency of this system in predicting the drug sensitivity of brain tumors. First, we compared the result of a drug sensitivity test using CAM of a chick embryo with that using nude mice. Next we studied the effect of chemotherapeutic agents against brain metastasis of a chick embryo caused by the intravenous injection of mouse B16 melanoma cells. The tumor reduction rate of the sensitivity test using a chick embryo tended to agree with that using nude mice. In the drug sensitivity test against brain metastasis, ACNU was the most effective. This result supports the result of the clinical study. In conclusion, the drug sensitivity test using a chick embryo is thought to be useful and the advantages or disadvantages of this system are discussed.
Nakamura
O
O
Department of Neurosurgery, Tokyo Metropolitan Komagome Hospital, Japan.
Sasaki
T
T
Uchida
H
H
Tanaka
M
M
Endo
Y
Y
Nishikawa
K
K
Matsutani
M
M
Takakura
K
K
D000482:Allantois; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001932:Brain Neoplasms / Q000473:pathology* / Q000556:secondary; D002642:Chick Embryo; D002823:Chorion; D004354:Drug Screening Assays, Antitumor; D005321:Extraembryonic Membranes; D005910:Glioma / Q000473:pathology*; D006801:Humans; D008546:Melanoma, Experimental / Q000473:pathology / Q000556:secondary; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D015376:Nimustine; D009607:Nitrosourea Compounds / Q000494:pharmacology
D000970:Antineoplastic Agents; D009607:Nitrosourea Compounds; D015376:Nimustine