Direct selection of Chinese hamster ovary strains deficient in CTP synthetase activity.
Mutant CHO cell strains dependent upon cytidine for growth and survival were isolated by a direct selection protocol. The mutants obtained are deficient in CTP synthetase activity (less than 2% residual activity) and have low reversion frequencies (less than 10(-7)). Cytidine deprivation of these stains leads to rapid depletion of intracellular CTP pools, but not dCTP pools, and a surprisingly rapid loss of cell viability. These properties should make the cytidine auxotrophs useful for a number of biochemical and genetic studies.
Kelsall
A
A
Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Potters Bar, Herts., U.K.
Meuth
M
M
D000818:Animals; D019731:Carbon-Nitrogen Ligases; D002460:Cell Line; D002470:Cell Survival; D006224:Cricetinae; D003412:Cricetulus; D003470:Culture Media; D003562:Cytidine / Q000378:metabolism; D008025:Ligases / Q000172:deficiency* / Q000235:genetics; D009154:Mutation; D012641:Selection, Genetic
D003470:Culture Media; D003562:Cytidine; D008025:Ligases; D019731:Carbon-Nitrogen Ligases; C019951:CTP synthetase