Restriction of measles virus gene expression in measles inclusion body encephalitis.
Measles virus (MV) infection in brain tissue of a patient with measles inclusion body encephalitis was characterized by immunologic and biochemical techniques. Of the five major structural proteins of MV, only nucleocapsid (N) protein and phosphoprotein (P protein) were consistently detected in diseased brain areas. In contrast, hemagglutinin protein was seen only occasionally, and no membrane and fusion proteins were found in any of the sections studied. Messenger RNAs (mRNAs) specific for these five viral proteins were detected in all brain extracts examined; however, the mRNAs for the envelope proteins were clearly underrepresented in comparison with lytically infected cells. Only the mRNAs for N and P proteins appeared active in in vitro translations. These findings indicate quantitative differences in the pattern of mRNA expression in brain tissue and a restricted expression of MV envelope proteins in infected cells as observed in subacute sclerosing panencephalitis.
Baczko
K
K
Institut für Virologie und Immunbiologie, Universität Würzburg, Federal Republic of Germany.
Liebert
U G
UG
Cattaneo
R
R
Billeter
M A
MA
Roos
R P
RP
ter Meulen
V
V
D001921:Brain / Q000382:microbiology; D004660:Encephalitis / Q000150:complications / Q000382:microbiology*; D005260:Female; D005786:Gene Expression Regulation; D005814:Genes, Viral; D006801:Humans; D007223:Infant; D007945:Leukemia, Lymphoid / Q000150:complications; D008457:Measles / Q000150:complications / Q000382:microbiology*; D008459:Measles virus / Q000235:genetics / Q000302:isolation & purification*; D012333:RNA, Messenger / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis; D014764:Viral Proteins / Q000032:analysis
D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins