Cell surface antigen of encapsulated Staphylococcus epidermidis SE-360 protects mice from homologous infection.
Cell surface antigen was mechanically extracted from encapsulated strain SE-360 of Staphylococcus epidermidis and purified by DEAE-Sephadex A 25 (Cl- form) ion exchange chromatography. This antigen manifested type-specific activity and major sugar constituents were galactose, glucose and N-acetyl-glucosamine at the molar ratio 1.00:9.05:1.65. alpha-D-glucosyl- and N-acetyl-glucosaminyl-residues were closely correlated to the antigenic determinant. In mice, protection against homologous microorganisms could be achieved by active immunization with thus purified antigen. Type-specific opsonin in rabbit anti-SE-360 serum could also be absorbed.
Ohshima
Y
Y
Department of Microbiology, St. Marianna University School of Medicine, Kawasaki, Japan.
D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis / Q000276:immunology* / Q000302:isolation & purification; D000954:Antigens, Surface / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification; D002852:Chromatography, Ion Exchange; D003429:Cross Reactions; D007106:Immune Sera / Q000276:immunology; D007110:Immunity, Active; D007114:Immunization; D007116:Immunization, Passive; D005779:Immunodiffusion; D051379:Mice; D011233:Precipitin Tests; D013203:Staphylococcal Infections / Q000517:prevention & control*; D013212:Staphylococcus epidermidis / Q000276:immunology*
D000942:Antigens, Bacterial; D000954:Antigens, Surface; D007106:Immune Sera