A portion of the Pf155/RESA antigen of Plasmodium falciparum is accessible on the surface of infected erythrocytes.
An investigation of antigens accessible to lactoperoxidase-catalysed cell surface iodination on intact Plasmodium falciparum-infected red blood cells (RBC) has identified a 125I-labelled antigen with an apparent size of about 155 kD. This labelled protein was specifically immunoprecipitated by the following antibodies: a rabbit antiserum and a mouse monoclonal antibody raised against a synthetic peptide comprising the 3',8-mer repeat EENVEHDA of the Pf155/RESA protein; a rabbit antiserum raised against a synthetic octapeptide comprising two copies of the 3',4-mer repeat EENV of the Pf155/RESA protein; and rabbit antisera against another synthetic peptide C(MYSNNNVED)2. The last antibody shows a strong reaction in asexual blood stage parasites with the Pf155/RESA antigen. While this antigen has been described previously as a submembrane component of the outer membrane of infected RBC, this report shows that at least part of its is accessible to the surface of both ring and late trophozoite-infected erythrocytes.
Saul
A
A
Malaria Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892.
Maloy
W L
WL
Rock
E P
EP
Howard
R J
RJ
D000595:Amino Acid Sequence; D000818:Animals; D000953:Antigens, Protozoan / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology; D004910:Erythrocyte Membrane / Q000276:immunology*; D004912:Erythrocytes / Q000469:parasitology*; D006384:Hemagglutination; D006801:Humans; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010455:Peptides / Q000737:chemistry / Q000276:immunology; D010963:Plasmodium falciparum / Q000276:immunology*; D015800:Protozoan Proteins / Q000276:immunology*
D000953:Antigens, Protozoan; D000954:Antigens, Surface; D010455:Peptides; D015800:Protozoan Proteins