[Establishment and characterization of three endometrial cancer cell lines from the same patient].
In order to determine some important cytological behavioral characteristics of endometrial cancer, I tried to establish endometrial cancer cell lines. Three endometrial cancer cell lines (KKNS-1, KKNS-2, KKNS-3) derived from the same patient have been established and successfully maintained in vitro for more than one year. The cells formed a monolayer in a mosaic fashion and pile up. Pathological findings for the tumor induced in athymic nude mice were: KKNS-1 was undifferentiated, KKNS-2 was differentiated and KKNS-3 was undifferentiated. Population doubling time was calculated to be about 35(KKNS-1), 60(KKNS-2), and 28(KKNS-3) hours. The modal chromosomal number for the cells fell in the diploid range. Estrogen receptor was demonstrated only in KKNS-2 by the ER-EIA and ER-ICA methods. HLA-expression was demonstrated, HLA-ABC was positive in all three lines and HLA-DR was positive only in KKNS-2. Anticancer drug sensitivity was demonstrated only in KKNS-3. Hormone sensitivity was demonstrated only in KKNS-2. We must therefore carefully treat endometrial cancer patients with anticancer drug or hormonal therapy whose pathological findings are heterogeneous.
Misawa
Y
Y
Department of Obstetrics and Gynecology, Niigata University School of Medicine.
D000328:Adult; D000970:Antineoplastic Agents / Q000494:pharmacology; D002455:Cell Division; D002460:Cell Line; D004354:Drug Screening Assays, Antitumor; D005260:Female; D006801:Humans; D011960:Receptors, Estrogen / Q000032:analysis; D014594:Uterine Neoplasms / Q000032:analysis / Q000473:pathology*
D000970:Antineoplastic Agents; D011960:Receptors, Estrogen