Rapid changes in levels of individual molecular forms of acetylcholinesterase after denervation of mouse sternocleidomastoid muscle.
Experimental denervation of adult mouse sternocleidomastoid muscle results in a decrease in total AChE. The most rapid change essentially affects the tailed, asymmetric 16 S AChE, since one day after nerve section, "16S" AChE is already significantly decreased to about 70% of its control value. We found that both background and junctional "16S" AChE are affected by this rapid decrease. Later, a sharp fall in "10S" and "4S" AChE occurs about seven days after denervation when muscle atrophy develops with loss of weight and proteins. A gaussian analysis of the sedimentation profiles of AChE extracted from denervated muscle shows that there is not only an early rapid decrease in 16 S AChE but also a decrease in the monomeric 3.3S AChE. This result suggests that there is a very rapid turn-over of two molecular forms of AChE, the supposedly monomeric precursor and the complex asymmetric 16S AChE.
Goudou
D
D
Blondet
B
B
Friboulet
A
A
Rieger
F
F
D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D001284:Atrophy; D002499:Centrifugation, Density Gradient; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D051379:Mice; D008815:Mice, Inbred Strains; D009121:Muscle Denervation; D009124:Muscle Proteins / Q000378:metabolism; D009132:Muscles / Q000201:enzymology* / Q000378:metabolism / Q000473:pathology; D009929:Organ Size; D011487:Protein Conformation
D009124:Muscle Proteins; D002795:Choline O-Acetyltransferase; D000110:Acetylcholinesterase