Crystallization of prostatic binding protein.
Prostatic binding protein is a dimeric glycoprotein capable of binding a variety of steroids. This protein is a major component of rat prostate cytosol making it possible to purify milligram quantities. Hexagonal crystals of X-ray diffraction quality have been grown from phosphate buffered ammonium sulfate solution by vapor diffusion methods. These crystals which are reasonably stable to X-rays, show diffraction to 6.3 A and belong to space group P6(1) or P6(1)22 or the enantiomorphs. The unit cell has dimensions a = 88.7(5) A, c = 405(2) A, contains 24 molecules and has a specific volume of 2.8 A3/Dalton.
Punzi
J S
JS
Medical Foundation of Buffalo, Inc., NY 14203.
Ghosh
D
D
Weeks
C M
CM
Van Roey
P
P
Duax
W L
WL
D000645:Ammonium Sulfate; D000727:Androgen-Binding Protein / Q000302:isolation & purification*; D000818:Animals; D011232:Chemical Precipitation; D002845:Chromatography; D003460:Crystallization; D003600:Cytosol / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D046911:Macromolecular Substances; D008297:Male; D008970:Molecular Weight; D011467:Prostate / Q000032:analysis*; D060789:Prostatein; D051381:Rats; D011920:Rats, Inbred WF; D060765:Secretoglobins; D014598:Uteroglobin; D014961:X-Ray Diffraction; D060788:Mammaglobin B
D000645:Ammonium Sulfate; D000727:Androgen-Binding Protein; D046911:Macromolecular Substances; D060789:Prostatein; D060765:Secretoglobins; D014598:Uteroglobin; C511484:Scgb1d2 protein, rat; C511483:Scgb1d4 protein, rat; C556468:Scgb2a1 protein, rat; D060788:Mammaglobin B