Enhancement of Ca2+-induced catecholamine release by the phorbol ester TPA in digitonin-permeabilized cultured bovine adrenal chromaffin cells.
The phorbol ester, 4 beta-phorbol 12-myristate acetate (TPA), increased the extent of catecholamine release induced by Ca2+, without affecting the basal release response in digitonin-permeabilized chromaffin cells. This finding is consistent with the hypothesis that protein kinase C has a role to play in stimulus-secretion coupling in the bovine adrenal medullary chromaffin cell.
Brocklehurst
K W
KW
Pollard
H B
HB
D000313:Adrenal Medulla / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D002395:Catecholamines / Q000378:metabolism*; D002417:Cattle; D002463:Cell Membrane Permeability / Q000187:drug effects; D002478:Cells, Cultured; D002838:Chromaffin System / Q000187:drug effects / Q000378:metabolism*; D004072:Digitonin / Q000494:pharmacology; D004560:Electricity; D010704:Phorbols / Q000494:pharmacology*; D011493:Protein Kinase C; D011494:Protein Kinases / Q000502:physiology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D002395:Catecholamines; D010704:Phorbols; D011494:Protein Kinases; D011493:Protein Kinase C; D004072:Digitonin; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium