Ethanol-induced alterations in exocrine pancreatic amino acid transport and secretion.
The effects of ethanol on exocrine pancreatic amino acid transport and secretion were investigated during perfusion of the isolated rat pancreas with ethanol concentrations ranging from 0.06% to 4.1%. Amino acid transport was quantitated using a rapid dual isotope dilution technique in which unidirectional substrate uptake (15-20 sec) is assessed relative to an extracellular tracer. Pancreatic secretion evoked by 0.3 microM carbachol was abolished during perfusion with 0.32% ethanol. Influx of L-lysine, L-serine and methylaminoisobutyric acid (MeAIB) was marginally increased by 0.32% ethanol but significantly inhibited during subsequent perfusion with 1.28-4.1% ethanol. Pancreatic oxygen consumption and effluent PCO2 levels decreased with increasing ethanol concentration, and the control venous pH (7.21 +/- 0.01, n = 8) gradually approached arterial pH values (7.46 +/- 0.02, n = 9). These results indicate that low concentrations of ethanol readily inhibit secretagogue-induced pancreatic secretion. Amino acid transport at the basolateral membrane of the exocrine pancreatic epithelium appears only to be inhibited after acute exposure to high ethanol concentrations.
Mann
G E
GE
Department of Physiology, King's College London, U.K.
Munoz
M
M
Peran
S
S
D000596:Amino Acids / Q000378:metabolism*; D000621:Aminoisobutyric Acids / Q000378:metabolism; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D004848:Epithelium / Q000378:metabolism; D000431:Ethanol / Q000494:pharmacology*; D008239:Lysine / Q000378:metabolism; D008297:Male; D010101:Oxygen Consumption / Q000187:drug effects; D010179:Pancreas / Q000378:metabolism*; D010477:Perfusion; D011312:Pressure; D051381:Rats; D011919:Rats, Inbred Strains; D012694:Serine / Q000378:metabolism
D000596:Amino Acids; D000621:Aminoisobutyric Acids; C017911:2-(methylamino)isobutyric acid; D000431:Ethanol; D012694:Serine; D008239:Lysine