Reorganization of cerebellar cell suspension transplanted into the weaver mutant cerebellum and immunohistochemical detection of synaptic formation.
Dissociated cells prepared from the cerebellar primordia of normal 15-day mouse embryos were grafted into the cerebellum of 1-month-old weaver mutant mice which are characterized by degeneration of cerebellar granule cells during the early postnatal period. The growth of the grafted cells was investigated at 1 month after the operation. Implanted cells were highly developed to form a large mass of tissue in the host cerebellar folia. Histological examination revealed that a trilaminar cortical structure was partially developed in certain areas of the grafted tissue. The implanted granule-like cells were labeled with [3H]thymidine which was injected into the host, suggesting that the granule-like cells actively proliferate in the host cerebellum after the transplantation. In this area, strong immunoreactivity with synapsin I was detected indicating that the dissociated granule cells of the cerebellar primordia are able to develop a synaptic organization in the weaver mouse cerebellum.
Kohsaka
S
S
Department of Physiology, School of Medicine, Keio University, Tokyo, Japan.
Takayama
H
H
Ueda
T
T
Toya
S
S
Tsukada
Y
Y
D000818:Animals; D002454:Cell Differentiation; D002531:Cerebellum / Q000032:analysis / Q000166:cytology / Q000637:transplantation*; D006085:Graft Survival; D007150:Immunohistochemistry; D051379:Mice; D008808:Mice, Inbred CBA; D008818:Mice, Neurologic Mutants / Q000502:physiology*; D009419:Nerve Tissue Proteins / Q000032:analysis; D013569:Synapses / Q000032:analysis / Q000502:physiology*; D016704:Synapsins; D013997:Time Factors
D009419:Nerve Tissue Proteins; D016704:Synapsins