title: Physical and genetic characterization of cloned enterobactin genomic sequences from Escherichia coli K-12.
abstract: We have cloned genes responsible for enterobactin synthesis (entD) and transport (fepA,fes) from Escherichia coli K-12. Relevant recombinant plasmids enabled EntD- and transport-defective mutants to grow on iron-limiting medium. Subcloning and deletion analysis demonstrated that the gene order is entD-fepA-fes. Protein synthesis studies in minicells suggest that FepA is first translated as an Mr 84 000 precursor, which is subsequently cleaved to the active Mr 81 000 receptor; the fes gene product is an Mr 44 000 protein; no polypeptide has been identified as the entD gene product.
authors.forename: T P
authors.initials: TP
authors.lastname: Fleming
authors.forename: M S
authors.initials: MS
authors.lastname: Nahlik
authors.forename: J B
authors.initials: JB
authors.lastname: Neilands
authors.forename: M A
authors.initials: MA
authors.lastname: McIntosh
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D001693:Biological Transport, Active; D002352:Carrier Proteins / Q000235:genetics*; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial / Q000648:ultrastructure; D003001:Cloning, Molecular; D004758:Enterobactin / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism / Q000648:ultrastructure; D005798:Genes, Bacterial; D007501:Iron / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D011956:Receptors, Cell Surface; D012694:Serine / Q000031:analogs & derivatives*
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D002352:Carrier Proteins; D011956:Receptors, Cell Surface; C029489:enterobactin receptor; D004758:Enterobactin; D012694:Serine; D007501:Iron