[Cultivation of the producer of alpha-amylase Bacillus subtilis under batch and continuous conditions].
The growth of Bacillus subtilis FU-79 and its production of alpha-amylase (EC 3.2.1.1) were studied under the conditions of batch and continuous cultivation in a semisynthetic medium. The enzyme activity fell down abruptly upon a pulse addition of either glucose or yeast extract to the chemostat culture, and remained at a low level for the following ten generations. Apparently, a double limitation of the culture growth (viz., with residual glucose and with yeast extract components) is required for the activity of alpha-amylase to be high.
Lirova
S A
SA
Ermakova
L M
LM
Rabotnova
I L
IL
Khovrychev
M P
MP
D001412:Bacillus subtilis / Q000201:enzymology / Q000254:growth & development*; D004794:Enzyme Repression; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D000516:alpha-Amylases / Q000096:biosynthesis*
D000516:alpha-Amylases
Kul'tivirovanie produtsenta alpha-amilazy Bacillus subtilis v periodicheskikh i nepreryvnykh usloviiakh.