Effect of estradiol and tamoxifen on the anchorage-independent growth of the subpopulations derived from MCF-7 breast carcinoma cells: cytogenetic analysis of the stem cell subpopulation.
The MCF-7 breast carcinoma cell line can be separated by Percoll density gradient centrifugation into several subpopulations, A to F, one of which (E) has been previously suggested to be highly enriched in stem cells. The anchorage-independent growth of the different fractions and its sensitivity to estradiol (E2) and tamoxifen (TAM) was assayed. The anchorage-independent growth capacity of the different fractions was E greater than A greater than B greater than D greater than C,F. The E fraction had the highest clonogenic index (6.62 +/- 1.18) and was unaffected by E2 or TAM. The karyotypic analysis of the E fraction revealed features similar to those of the unfractionated cell line. It is suggested that the high growth rate of fraction E is due to an enrichment in stem cells and not to the existence of a different clone.
Podhajcer
O L
OL
Hospital Mariano R. Castex, San Martín, Provincia de Buenos Aires, Argentina.
Resnicoff
M
M
Bover
L
L
Medrano
E E
EE
Slavutsky
I
I
Larripa
I
I
Mordoh
J
J
D001943:Breast Neoplasms / Q000235:genetics / Q000473:pathology*; D002448:Cell Adhesion / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D004958:Estradiol / Q000494:pharmacology*; D005819:Genetic Markers; D006801:Humans; D007621:Karyotyping; D014411:Neoplastic Stem Cells / Q000166:cytology / Q000187:drug effects*; D013629:Tamoxifen / Q000494:pharmacology*
D005819:Genetic Markers; D013629:Tamoxifen; D004958:Estradiol