title: Demonstration and partial characterization of glutathione disulfide-stimulated ATPase activity in the plasma membrane fraction from rat hepatocytes.
abstract: A highly purified plasma membrane fraction isolated from rat hepatocytes was found to catalyze the hydrolysis of ATP in response to micromolar concentrations of glutathione disulfide (GSSG). This process exhibited distinct kinetic parameters suggesting the existence of both a high and low affinity component. The apparent Km values (GSSG) for ATP hydrolysis were 140 microM and 1 mM for the high affinity and low affinity components, respectively. Disulfides other than GSSG were also found to stimulate ATP hydrolysis. The similarity between the kinetic properties of the GSSG-stimulated ATPase and those reported for GSSG transport in erythrocytes (Kondo, T., Dale, G. L., and Beutler, E. (1980) Proc. Natl. Acad. Sci. U.S.A. 77, 6359-6362) suggests that the ATPase may function in the active extrusion of intracellular GSSG.
authors.forename: P
authors.initials: P
authors.lastname: Nicotera
authors.forename: M
authors.initials: M
authors.lastname: Moore
authors.forename: G
authors.initials: G
authors.lastname: Bellomo
authors.forename: F
authors.initials: F
authors.lastname: Mirabelli
authors.forename: S
authors.initials: S
authors.lastname: Orrenius
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001693:Biological Transport, Active; D002458:Cell Fractionation; D002462:Cell Membrane / Q000201:enzymology / Q000648:ultrastructure; D004220:Disulfides / Q000494:pharmacology; D005978:Glutathione / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D019803:Glutathione Disulfide; D007700:Kinetics; D008099:Liver / Q000201:enzymology* / Q000648:ultrastructure; D008297:Male; D008855:Microscopy, Electron, Scanning; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D004220:Disulfides; D000255:Adenosine Triphosphate; D000251:Adenosine Triphosphatases; D005978:Glutathione; D019803:Glutathione Disulfide