A heritable variant of mouse liver ornithine aminotransferase (EC 2.6.1.13) induced by ethylnitrosourea.
A variant of ornithine aminotransferase (OAT, EC 2.6.1.13) has been detected in an offspring of a male mouse treated with ethylnitrosourea. The evidence presented to support the identification of the protein variant (ENU 2) as altered OAT includes (a) a corresponding 50% decrease in the abundance of a protein, located one charge unit basic to the variant, which comigrates on two-dimensional gel patterns with purified mouse liver OAT; (b) the binding of anti-rat-OAT antibody to the variant; (c) the increased abundance of the variant protein in the livers of mice fed a high protein diet (85% casein); and (d) purification of the variant through an OAT purification protocol.
Giometti
C S
CS
Biological, Environmental, and Medical Research Division, Argonne National Laboratory, Illinois 60439.
Tollaksen
S L
SL
Gemmell
M A
MA
Burcham
J
J
Peraino
C
C
D000818:Animals; D004044:Dietary Proteins / Q000008:administration & dosage / Q000494:pharmacology; D004790:Enzyme Induction; D005038:Ethylnitrosourea / Q000494:pharmacology*; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008297:Male; D051379:Mice; D009154:Mutation; D009953:Ornithine-Oxo-Acid Transaminase / Q000096:biosynthesis* / Q000235:genetics; D000637:Transaminases / Q000096:biosynthesis*
D004044:Dietary Proteins; D000637:Transaminases; D009953:Ornithine-Oxo-Acid Transaminase; D005038:Ethylnitrosourea