title: Low- and intermediate-copy-number cloning vectors based on the Pseudomonas plasmid pVS1.
abstract: The cloning vector pME290 (6.8 kb), which is derived from the Pseudomonas plasmid pVS1 and has about 7 copies, was mutagenized in vitro to provide derivatives with altered copy numbers. Thus, pME292 (about 1-3 copies) and pME294 (about 15-20 copies) were isolated. These vectors were used in the characterization of the P. aeruginosa argF gene encoding ornithine carbamoyltransferase.
authors.affiliation: Dept. of Bacteriology, Shinshu University School of Medicine, Matsumoto, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Itoh
authors.forename: L
authors.initials: L
authors.lastname: Soldati
authors.forename: T
authors.initials: T
authors.lastname: Leisinger
authors.forename: D
authors.initials: D
authors.lastname: Haas
mesh_terms: D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000648:ultrastructure; D004274:DNA, Recombinant / Q000648:ultrastructure; D005786:Gene Expression Regulation; D005822:Genetic Vectors; D010957:Plasmids; D011550:Pseudomonas aeruginosa / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; D004274:DNA, Recombinant