title: Post-translational transport of proteins into microsomal membranes of Candida maltosa.
abstract: We have isolated from the yeast Candida maltosa microsomal membranes that are active in the translocation of proteins synthesized in cell-free systems derived from C. maltosa, Saccharomyces cerevisiae or wheat germ. Translocation and core glycosylation of prepro-alpha-factor, a secretory protein, were observed with yeast microsomes added during or after translation. The signal peptide is cleaved off. Cytochrome P-450 from C. maltosa, the first integral membrane protein studied in a yeast system, is also inserted both co- and post-translationally into Candida microsomal membranes. Its insertion into canine microsomes occurs efficiently only in a co-translational manner and is dependent on the function of the signal recognition particle.
authors.affiliation: Zentralinstitut für Molekularbiologie, Akademie der Wissenschaften der DDR, Berlin-Buch.
authors.forename: M
authors.initials: M
authors.lastname: Wiedmann
authors.forename: B
authors.initials: B
authors.lastname: Wiedmann
authors.forename: S
authors.initials: S
authors.lastname: Voigt
authors.forename: E
authors.initials: E
authors.lastname: Wachter
authors.forename: H G
authors.initials: HG
authors.lastname: Müller
authors.forename: T A
authors.initials: TA
authors.lastname: Rapoport
mesh_terms: D002175:Candida / Q000378:metabolism*; D002474:Cell-Free System; D005656:Fungal Proteins / Q000378:metabolism*; D006031:Glycosylation; D007425:Intracellular Membranes / Q000378:metabolism; D000072235:Mating Factor; D008565:Membrane Proteins / Q000378:metabolism*; D008861:Microsomes / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism; D011498:Protein Precursors / Q000378:metabolism; D011499:Protein Processing, Post-Translational
chemical_list: D005656:Fungal Proteins; D008565:Membrane Proteins; D010455:Peptides; D011498:Protein Precursors; D000072235:Mating Factor