title: 400-MHz 1H-NMR spectroscopy of fucosylated tetrasialyl oligosaccharides isolated from normal and cirrhotic alpha 1-acid glycoprotein.
abstract: The comparative study of fucosylated tetrasialyl-oligosaccharides isolated from normal and cirrhotic alpha 1-AGP was performed using permethylation and 400-MHz 1H-NMR spectroscopy. These results clearly show the tetraantennary structure of these two oligosaccharides with hyperfucosylation for the tetrasialylated fraction from cirrhotic alpha 1-AGP. In the latter oligosaccharide the simultaneous presence on two antennae (7 and 7') of the sialosyl Lewis X determinant NeuAc-(alpha 2-3) Gal(beta 1-4) [Fuc(alpha 1-3)] GlcNAc has been observed. Moreover the 5 and 5' antennae were alpha 2-6 sialylated but without fucose.
authors.affiliation: Laboratoire de Chimie Biologique et Unité Associée au CNRS no. 217, Université des Sciences et Techniques de Lille Flandres-Artois, Villeneuve d'Ascq, France.
authors.forename: J M
authors.initials: JM
authors.lastname: Wieruszeski
authors.forename: B
authors.initials: B
authors.lastname: Fournet
authors.forename: D
authors.initials: D
authors.lastname: Konan
authors.forename: D
authors.initials: D
authors.lastname: Biou
authors.forename: G
authors.initials: G
authors.lastname: Durand
mesh_terms: D001202:Ascitic Fluid / Q000032:analysis; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000032:analysis; D005643:Fucose / Q000032:analysis*; D006801:Humans; D008103:Liver Cirrhosis / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000032:analysis*; D009961:Orosomucoid / Q000032:analysis*; D012794:Sialic Acids / Q000032:analysis
chemical_list: D002241:Carbohydrates; D009844:Oligosaccharides; D009961:Orosomucoid; D012794:Sialic Acids; D005643:Fucose