DNA fingerprinting in domestic animals using four different minisatellite probes.
Four probes known to allow DNA fingerprinting in the human (M13, Jeffreys' core sequence, the human alpha globin hypervariable region [HVR], and a mouse probe related to the Drosophila Per gene) were checked for their ability to reveal "genetic bar codes" in cattle, horses, pigs, dogs, chickens, and a European cyprinid fish, the barbel (Barbus barbus L.). Individual-specific patterns were obtained in cattle using M13, Jeffreys' core sequence, and the alpha globin HVR, in horses, dogs, and pigs using M13, Jeffreys' core sequence, and the Per probe, and in chicken and fish using the four different probes. Although we observed a considerable heterogeneity in the extent of interindividual variation, depending on the particular probe-species combination, the fingerprints are polymorphic enough to be used efficiently in animal identification, paternity testing, and as a source of genetic markers for linkage analysis. These markers should substantially accelerate the mapping of genes affecting economically important traits.
Georges
M
M
Chaire de Génétique, Faculté de Médecine Vétérinaire, Université de Liège, Bruxelles.
Lequarré
A S
AS
Castelli
M
M
Hanset
R
R
Vassart
G
G
D000818:Animals; D000829:Animals, Domestic / Q000235:genetics*; D002874:Chromosome Mapping; D004247:DNA / Q000235:genetics*; D004276:DNA, Satellite / Q000235:genetics; D005819:Genetic Markers; D009693:Nucleic Acid Hybridization; D009710:Nucleotide Mapping; D013045:Species Specificity
D004276:DNA, Satellite; D005819:Genetic Markers; D004247:DNA