The effect of short-term temperature changes on the adhesivity of nerve cells. Participation of DNA molecules on non-specific cellular adhesion.
Brain cells from 16 to 18-day-old mice embryos were dissociated by mild trypsinization and sieving. Immediately after dissociation the cells were preincubated in a PBS solution at -6 to +54 degrees C for 3 and 20 min. After this preincubation cells were rotated for 60 min at 37 degrees C in the PBS solution. Cellular adhesivity was estimated during this time period and EM pictures of organized in vitro aggregates after 24-28 h were taken. In a separate series of experiments, freshly dissociated were treated with DNAase before the rotation procedure. Preincubation in a cold or a warm medium did not alter the inhibition of cellular adhesivity significantly. Distinct inhibition of cellular adhesion was observed in cells preincubated above 53 degrees C. Adhesion was also inhibited below -5 degrees C, however, this effect was mainly dependent on the rate of freezing and thawing. Digestion of dissociated cells with DNAase (20 micrograms/ml) decreased cell adhesion. At 37 degrees C the adhesivity decreased by about 20%. Aggregates of cells preincubated at 0 degrees C for 20 min did not exhibit marked EM changes after 24-28 h in vitro. The present results have shown the rather high resistance of molecules responsible for cellular adhesion and its reversibility to temperature changes. Furthermore, non-specific cellular adhesion was shown on physically active DNA molecules.
Lodin
Z
Z
Hajková
E
E
Hartman
J
J
Faltin
J
J
Srajer
J
J
D000818:Animals; D001921:Brain / Q000166:cytology / Q000196:embryology; D002448:Cell Adhesion / Q000187:drug effects; D002449:Cell Aggregation; D003584:Cytological Techniques; D004247:DNA / Q000494:pharmacology*; D003851:Deoxyribonucleases; D051379:Mice / Q000196:embryology; D009474:Neurons / Q000502:physiology*; D013696:Temperature
D004247:DNA; D003851:Deoxyribonucleases