Cerebrospinal fluid lymphocytes from HIV-infected patients synthesize HIV-specific antibody in vitro.
Cerebrospinal fluid lymphocytes from six human immunodeficiency virus (HIV)-infected and three seronegative patients were studied for in vitro synthesis of HIV-specific antibody by means of Western blot analysis of lymphocyte culture supernatants. All the HIV-positive subjects showed in vitro production of HIV-specific IgG, regardless of neurological involvement, while no specific antibody synthesis was detected in seronegative individuals. These data provide the first direct evidence that HIV-specific antibody in cerebrospinal fluid is due to intrathecal synthesis.
Amadori
A
A
Institute of Oncology, University of Padova, Italy.
De Rossi
A
A
Gallo
P
P
Tavolato
B
B
Chieco-Bianchi
L
L
D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology*; D000914:Antibodies, Viral / Q000096:biosynthesis* / Q000302:isolation & purification; D002555:Cerebrospinal Fluid / Q000276:immunology* / Q000473:pathology; D006678:HIV / Q000276:immunology; D015483:HIV Antibodies; D006679:HIV Seropositivity / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis; D008214:Lymphocytes / Q000276:immunology*
D000914:Antibodies, Viral; D015483:HIV Antibodies; D007074:Immunoglobulin G