Defective autologous mixed lymphocyte reactions in patients with renal insufficiency--evidence for cellular and serum factors.
The autologous mixed lymphocyte reaction (AMLR) was studied in 10 patients who were azotemic from renal diseases not considered to be immunologically mediated. These patients were not on chronic dialysis. The AMLR was significantly depressed in patients with azotemia when compared to the AMLR in normals. When the AMLR was performed utilizing lymphocytes from normals, the proliferative response was markedly decreased if azotemic serum was substituted for normal serum. However, when the AMLR was performed utilizing lymphocytes from azotemic patients, the proliferative response did not significantly improve if normal serum was substituted for azotemic serum. In addition, the proliferative responses of T cells to concanavalin A (Con A) and phytohemagglutinin (PHA) were not significantly depressed in azotemic patients when compared to normal controls. These data suggest that the AMLR is abnormal in azotemic patients because of an intrinsic defect in the mononuclear cells, possibly in the stimulating non-T cells, and an inhibitory factor in the serum.
Frymoyer
P A
PA
Davey
F R
FR
D006801:Humans; D007674:Kidney Diseases / Q000276:immunology*; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D014511:Uremia / Q000097:blood