title: Glucose both inhibits and stimulates insulin secretion from isolated pancreatic islets exposed to maximally effective concentrations of sulfonylureas.
abstract: Isolated pancreatic islets from mice were perifused with media containing maximally effective concentrations of glibenclamide (0.1-10 mumol/l) or glipizide (1 mumol/l). In these islets an increase of the glucose concentration from 10 mmol/l to 40 mmol/l or addition of D-glyceraldehyde (20 mmol/l) caused a temporary decrease in insulin release which was followed by a sustained enhancement of release. alpha-Ketoisocaproate (3 or 20 mmol/l) did not inhibit insulin release; at high concentration it was an even stronger secretagogue than D-glucose or D-glyceraldehyde. It is concluded that high energy phosphates couple B-cell fuel metabolism and insulin release by acting both on the ATP-dependent K+ channel and on other targets not yet identified.
authors.affiliation: Institut für Pharmakologie und Toxikologie, Universität Göttingen, Federal Republic of Germany.
authors.forename: U
authors.initials: U
authors.lastname: Panten
authors.forename: M
authors.initials: M
authors.lastname: Schwanstecher
authors.forename: A
authors.initials: A
authors.lastname: Wallasch
authors.forename: S
authors.initials: S
authors.lastname: Lenzen
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D005913:Glipizide / Q000494:pharmacology; D005947:Glucose / Q000494:pharmacology*; D005905:Glyburide / Q000494:pharmacology; D066298:In Vitro Techniques; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007457:Iodine Radioisotopes; D007515:Islets of Langerhans / Q000187:drug effects / Q000378:metabolism*; D007651:Keto Acids / Q000494:pharmacology; D008297:Male; D051379:Mice; D013453:Sulfonylurea Compounds / Q000494:pharmacology*
chemical_list: D007328:Insulin; D007457:Iodine Radioisotopes; D007651:Keto Acids; D013453:Sulfonylurea Compounds; C013082:alpha-ketoisocaproic acid; D005947:Glucose; D005905:Glyburide; D005913:Glipizide