[Enhancement of the combination of F-actin and alpha-actinin association in the presence of 1,2-propanediol].
The influence of 1,2-propanediol on the G-actin/F-actin equilibrium has been studied previously. We report here its effect on the crosslinking of actin filaments by alpha-actinin. Capillary viscometry experiments indicate a marked increase in the viscosity of F-actin/alpha-actinin solutions in the presence of this solvent, with a rise of the gel point temperature. Gel electrophoresis obtained after high-speed sedimentation of these proteins showed an enhancement of the alpha-actinin/actin filaments association. Propanediol-induced perturbations of interactions between microfilaments and actin-binding proteins might help explain the variability of its cryoprotective efficiency for the preservation of various cells.
Nguyen
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E
Unité de Recherche en Développement concerté I.N.S.E.R.M.-I.N.R.A. n. 310, Institut de Biologie physico-chimique, Paris.
Pajot-Augy
E
E
Campion
E
E
Prulière
G
G
D000185:Actinin / Q000378:metabolism*; D000199:Actins / Q000378:metabolism*; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D004347:Drug Interactions; D066298:In Vitro Techniques; D011108:Polymers; D019946:Propylene Glycol; D011409:Propylene Glycols / Q000494:pharmacology*; D011817:Rabbits; D014783:Viscosity
D000199:Actins; D000894:Anti-Inflammatory Agents, Non-Steroidal; D011108:Polymers; D011409:Propylene Glycols; D000185:Actinin; D019946:Propylene Glycol
Augmentation de l'association F-actine/alpha-actinine en présence de 1,2-propanediol.