title: Micropruning: the mechanism of turning of Aplysia growth cones at substrate borders in vitro.
abstract: Growth cones of Aplysia californica neurons were observed with video-enhanced contrast-differential interference contrast (VEC-DIC) microscopy as they turned at a border between poly-L-lysine-treated and untreated glass. Growth cones that turned generally developed 2 distinct active areas of filopodial and veil formation, much in the way of growth cones undergoing branching. Both active areas advanced, but turning of the neurite occurred through the selective resorption of the incipient branches developing on the untreated substrate. Thus, micropruning of developing regions of the growth cone, rather than the asymmetric extension of filopodia or veils, was primarily responsible for directing neurite growth. We present the hypothesis that abrupt turns by growing neurites are mediated by 2 sets of signals, one causing growth cone splitting, and a second set regulating the survival of the separate branches.
authors.affiliation: Department of Pharmacology, Columbia University College of Physicians and Surgeons, New York, New York 10032.
authors.forename: D W
authors.initials: DW
authors.lastname: Burmeister
authors.forename: D J
authors.initials: DJ
authors.lastname: Goldberg
mesh_terms: D000818:Animals; D001048:Aplysia; D001369:Axons / Q000502:physiology*; D001696:Biomechanical Phenomena; D003287:Contrast Media; D005898:Glass; D007089:Image Enhancement; D008857:Microscopy, Interference; D009474:Neurons / Q000502:physiology / Q000648:ultrastructure; D011107:Polylysine / Q000494:pharmacology; D013690:Television
chemical_list: D003287:Contrast Media; D011107:Polylysine