Open canalicular system of platelets in porcine stress syndrome.
A study was undertaken to test whether a previously reported alteration in platelet morphology could be of predictive value for the detection of stress-susceptibility in pigs. Platelets from 20 normal pigs, nine pigs classified as stress-susceptible on the basis of their response to halothane challenge, and 11 siblings of halothane reactors belonging to two different breeds were subjected to electron microscopic examination. A quantitative analysis of electron micrographs, based on the extent of dilatation of the open canalicular system in platelets and the percentage of affected platelets, revealed that halothane reactor pigs could be distinguished from normal animals on the basis of their open canalicular system score. The discrete nature of the score categories in siblings indicates that platelet alteration may be an inherent component of the porcine stress syndrome and suggests that some of the false negatives in the halothane test may be identified as stress-susceptible on this criterion. Further studies involving a larger number of halothane reactors and siblings are needed to ascertain the consistency of the open canalicular system features and eventually, to develop a simple test system based on platelet alterations for the detection of stress-susceptibility in pigs.
Basrur
P K
PK
Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph.
Bouvet
A
A
McDonell
W N
WN
D000818:Animals; D001792:Blood Platelets / Q000648:ultrastructure*; D006221:Halothane; D008305:Malignant Hyperthermia / Q000097:blood / Q000175:diagnosis / Q000662:veterinary*; D008854:Microscopy, Electron; D013312:Stress, Physiological / Q000097:blood / Q000175:diagnosis / Q000662:veterinary*; D013552:Swine; D013553:Swine Diseases / Q000097:blood* / Q000175:diagnosis; D013577:Syndrome / Q000662:veterinary
D006221:Halothane