Chromosomal translocation involving the beta T cell receptor gene in acute leukemia.
DNA spanning a t(7;19) chromosomal translocation breakpoint was isolated from the human T cell line SUP-T7 established from an acute lymphoblastic leukemia. Nucleotide sequence analysis showed that the point of crossover on chromosome 7 occurred immediately adjacent to joining segment J beta 1.1 within the TCR-beta gene, suggesting that this translocation resulted from an error in TCR gene rearrangement. On chromosome 19, the translocation occurred within a previously uncharacterized transcriptional unit for which we propose the name lyl-1. An approximately 1.5-kb RNA is transcribed from this gene in a wide variety of hematolymphoid cell lines. The t(7;19) results in truncation of the lyl-1 gene and production of abnormal-sized RNAs, suggesting a role for lyl-1 in the pathogenesis of this leukemia.
Cleary
M L
ML
Department of Pathology, Stanford University School of Medicine, California 94305.
Mellentin
J D
JD
Spies
J
J
Smith
S D
SD
D001483:Base Sequence; D002888:Chromosomes, Human, Pair 19; D002897:Chromosomes, Human, Pair 7; D003001:Cloning, Molecular; D006801:Humans; D007945:Leukemia, Lymphoid / Q000235:genetics*; D008969:Molecular Sequence Data; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*; D014158:Transcription, Genetic; D014178:Translocation, Genetic
D011948:Receptors, Antigen, T-Cell