Immunoaffinity isolation of the Lewis blood group antigens.
Extraction, followed by immunoaffinity chromatography on a column of immobilized antibodies is described for the isolation of the Lewis blood group antigens. These affinity-isolated antigens are suitable in organ transplant immunology laboratories for screening potential donors and recipients for mismatch at the Lewis antigen loci.
Frantz
S C
SC
Immunochemistry Laboratory, George Washington University Medical Center, Washington, DC 20037.
Henriques
H F
HF
Phillips
T M
TM
D000918:Antibody Specificity; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D006801:Humans; D005779:Immunodiffusion; D007075:Immunoglobulin M / Q000302:isolation & purification; D007519:Isoantigens / Q000302:isolation & purification*; D007983:Lewis Blood Group Antigens
D007075:Immunoglobulin M; D007519:Isoantigens; D007983:Lewis Blood Group Antigens