Identification of mRNA for relaxin in the endometrium of the pregnant guinea-pig.
The endometrium of late pregnant guinea-pigs was found to contain mRNA for relaxin. Poly(A)+RNA hybridized to 32P-labelled porcine relaxin-specific oligonucleotide probes. These probes corresponded to the C-peptide region of the prohormone. There was no hybridization to a 32P-labelled probe to the B-chain of porcine relaxin even under conditions of low stringency. The size of the relaxin mRNA was approximately 1.0 kb and similar to that found for relaxin mRNA from the pregnant sow ovary. This is the first study on relaxin mRNA from a uterine source.
Tashima
L
L
Department of Anatomy and Reproductive Biology, University of Hawaii, Honolulu 96822.
Greenwood
F C
FC
Bryant-Greenwood
G D
GD
Peaker
M
M
D000818:Animals; D001483:Base Sequence; D004717:Endometrium / Q000032:analysis*; D005260:Female; D006168:Guinea Pigs; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D011247:Pregnancy; D012333:RNA, Messenger / Q000032:analysis*; D012065:Relaxin / Q000235:genetics*
D012333:RNA, Messenger; D012065:Relaxin