Specific induction of Ca2+ transport activity in MATa cells of Saccharomyces cerevisiae by a mating pheromone, alpha factor.
Incubation with a high concentration of a mating pheromone, alpha factor, of Saccharomyces cerevisiae induced the accumulation of Ca2+ ion in MATa cells, but not in MAT alpha or MATa/alpha cells, after a lag of 30-40 min. The alpha factor did not cause a nonspecific lesion of the membrane barrier, but induced Ca2+ transport activity specifically. This induction of Ca2+ transport activity correlated with formation of a projection on the cells, or with localized cell elongation, but not with G1 arrest or agglutinin induction. The increased Ca2+ transport activity was maintained only in the continuous presence of a high concentration of alpha factor and de novo protein synthesis. Kinetic studies of induction of Ca2+ transport by alpha factor and effects of ethylene glycol bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid and Ca2+ ionophore, A23187, on mating suggested an essential role of this physiological reaction in the initiation of sexual conjugation.
Ohsumi
Y
Y
Anraku
Y
Y
D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D000252:Calcium-Transporting ATPases / Q000096:biosynthesis*; D003513:Cycloheximide / Q000494:pharmacology; D004790:Enzyme Induction; D007700:Kinetics; D000072235:Mating Factor; D010455:Peptides / Q000502:physiology*; D010675:Pheromones / Q000502:physiology*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000378:metabolism*; D013045:Species Specificity
D010455:Peptides; D010675:Pheromones; D000072235:Mating Factor; D003513:Cycloheximide; D000252:Calcium-Transporting ATPases; D002118:Calcium