title: Synthetic peptides competitively inhibit both direct binding to fibroblasts and functional biological assays for the purified cell-binding domain of fibronectin.
abstract: The 75,000-dalton cell-binding domain of fibronectin (f75k) and synthetic peptides derived from its sequence have been used to examine the cell-surface fibronectin receptor. The spreading of baby hamster kidney fibrolasts on f75k-coated substrates and the direct binding of [3H]f75k to these cells were both competitively inhibited by a synthetic peptide of fibronectin with the sequence Gly-Arg-Gly-Asp-Ser, indicating that the peptide and f75k interact with the same cell-surface sites. Related peptides, including the inverted sequence Ser-Asp-Gly-Arg, also competed for f75k binding. Our results provide the first evidence that fibroblastic baby hamster kidney cells recognize the same fibronectin amino acid sequence in direct binding of soluble ligand and in indirect inhibitory biological assays, thus correlating our direct fibronectin-receptor binding assay with biological functional assays for the fibronectin receptor in fibroblasts.
authors.forename: S K
authors.initials: SK
authors.lastname: Akiyama
authors.forename: K M
authors.initials: KM
authors.lastname: Yamada
mesh_terms: D000818:Animals; D001667:Binding, Competitive; D002460:Cell Line; D006224:Cricetinae; D005347:Fibroblasts / Q000378:metabolism; D005353:Fibronectins / Q000378:metabolism*; D007668:Kidney; D007700:Kinetics; D008970:Molecular Weight; D010455:Peptides / Q000494:pharmacology*; D017469:Receptors, Fibronectin; D011971:Receptors, Immunologic / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D005353:Fibronectins; D010455:Peptides; D017469:Receptors, Fibronectin; D011971:Receptors, Immunologic