title: Properties of acid beta-galactosidase from fowl spermatozoa.
abstract: 1. Two forms, I and II, of beta-galactosidase [EC 3.2.1.23] from fowl spermatozoa were separated by Blue-Sepharose CL-6B chromatography. 2. The two forms of the enzyme yielded different pI values (4.1 and 4.7 for form I and 5.3 for form II). 3. Their gel filtration patterns were also different: form I resolved into two peaks with Mr about 65,000 and 80,000, whereas form II resolved into a single peak with an Mr of about 65,000. 4. Both forms had similar optimum pH, pH stability, thermal stability and Km values. 5. Differences between the characteristics of beta-galactosidase forms from spermatozoa and from seminal plasma suggest that the beta-galactosidases investigated in this study are spermatozoa-specific.
authors.affiliation: Department of Experimental Research, Institute of Animal Production, Chorzelów, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Droba
mesh_terms: D000818:Animals; D002645:Chickens / Q000502:physiology*; D002850:Chromatography, Gel; D005696:Galactosidases / Q000032:analysis*; D007525:Isoelectric Focusing; D008297:Male; D013094:Spermatozoa / Q000201:enzymology*; D001616:beta-Galactosidase / Q000032:analysis*
chemical_list: D005696:Galactosidases; D001616:beta-Galactosidase