Identification of 17 alpha,20 alpha-dihydroxyprogesterone in testicular extracts after incubation with ketoconazole.
The antifungal ketoconazole affects testosterone synthesis in dispersed rat testicular cells. In the presence of ketoconazole an accumulation of 17 alpha,20 alpha-dihydroxyprogesterone has been observed. This steroid was isolated from the testis of Wistar rats after a [4-14C]progesterone incorporation in the presence of ketoconazole. Its identification was achieved from the gas chromatographic/mass spectrometric analysis of the isolated radioactive fraction. A chemical derivatization of the fraction with butylboronic acid followed by mass spectrometric analysis confirmed the presence of 17 alpha,20 alpha-dihydroxyprogesterone.
Lauwers
W F
WF
Le Jeune
L
L
Vanden Bossche
H
H
Willemsens
G
G
D000818:Animals; D002250:Carbon Radioisotopes; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006908:Hydroxyprogesterones / Q000096:biosynthesis* / Q000302:isolation & purification; D007654:Ketoconazole / Q000494:pharmacology*; D008297:Male; D011374:Progesterone / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013737:Testis / Q000187:drug effects / Q000378:metabolism*
D002250:Carbon Radioisotopes; D006908:Hydroxyprogesterones; C007433:17,20-dihydroxy-4-pregnen-3-one; D011374:Progesterone; D007654:Ketoconazole