Cysteine proteinase inhibitors in inflamed human gingiva.
In the present work we demonstrate the presence of cysteine proteinase inhibitors of all three classes: kininogens, stefin A, and cystatin C, in inflamed human gingiva. Using cystatin C, in inflamed human gingiva. Using immunochemical methods we found that stefin A is the major inhibitor of cysteine proteinases, followed by kininogen and cystatin C. The values for stefin A and cystatin C ranged from 7.0--400 micrograms/g and 1.5--6.1 micrograms/g tissue, respectively, as determined by enzyme-linked immunosorbent assay in inflamed gingival homogenates from patients with different degrees of periodontal disease.
Babnik
J
J
Department of Biochemistry, J. Stefan Institute, E. Kardelj University, Ljubljana, Yugoslavia.
Curin
V
V
Lah
T
T
Turk
V
V
Skaleric
U
U
D000917:Antibody Formation; D002846:Chromatography, Affinity; D055313:Cystatin B; D015891:Cystatins; D015853:Cysteine Proteinase Inhibitors; D004797:Enzyme-Linked Immunosorbent Assay; D005881:Gingiva / Q000201:enzymology*; D005891:Gingivitis / Q000201:enzymology*; D006801:Humans; D007120:Immunochemistry; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D007525:Isoelectric Focusing; D011480:Protease Inhibitors / Q000276:immunology / Q000302:isolation & purification* / Q000494:pharmacology; D011506:Proteins / Q000302:isolation & purification
C524994:CSTB protein, human; D015891:Cystatins; D015853:Cysteine Proteinase Inhibitors; D011480:Protease Inhibitors; D011506:Proteins; D055313:Cystatin B