Human cells allosensitized in vitro release soluble suppressor factors: presence of at least two distinct factors.
Human cells allosensitized in vitro release suppressor "factors" (SF) capable of inhibiting a primary mixed lymphocyte reaction (MLR). Preincubation experiments with SF of either responders or stimulators in MLR suggested the presence of at least two distinct suppressor activities: one called SFR, acting on certain responders including the SF producer; the other called SFNR, acting on stimulators in all MLR. These activities can be separated using sorbents composed of monoclonal anti-HLA-DR antibodies on the one hand and mouse or rabbit IgG on the other hand. SFR is found in the effluent and SFNR in the eluate of these sorbents. The SFNR-binding properties are shared by a murine suppressor factor called immunoglobulin-binding factor or IBF. Moreover SFNR, like IBF, is capable of suppressing a secondary in vitro IgG response through the species barrier.
Bensussan
A
A
Kalil
J E
JE
Alvarez-Lopez
M R
MR
Bouchard
B
B
Fridman
W H
WH
Fellous
M
M
Sasportes
M
M
D000818:Animals; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D006801:Humans; D007114:Immunization; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D008222:Lymphokines / Q000032:analysis*; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D029584:Prostatic Secretory Proteins; D013491:Suppressor Factors, Immunologic; D013601:T-Lymphocytes / Q000276:immunology*
D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II; D008222:Lymphokines; D029584:Prostatic Secretory Proteins; D013491:Suppressor Factors, Immunologic; C043076:beta-microseminoprotein; C013059:immunoglobulin-binding factors