Simulation of vascular surfaces: differential saturation with glycosaminoglycans and their quantitative analysis.
Surface layers of sulphated glycosaminoglycan can be quantified by X-ray microanalysis using the local mass-fraction of the element sulphur. As a calibration standard radiolabelled chondroitin sulphate has been attached covalently to a nylon surface at various densities to the point where the molecules are packed as close as the radius of gyration permits.
Hale
G
G
Department of Biochemistry, University College, Cardiff, UK.
Hann
A C
AC
Wusteman
F S
FS
Gacesa
P
P
D000107:Acetylation; D002807:Chondroitin / Q000031:analogs & derivatives; D002809:Chondroitin Sulfates; D004577:Electron Probe Microanalysis; D004730:Endothelium, Vascular; D008854:Microscopy, Electron; D008954:Models, Biological; D009757:Nylons; D014316:Tritium
D009757:Nylons; D014316:Tritium; D002807:Chondroitin; D002809:Chondroitin Sulfates