A family of immunologically related transcription factors that includes multiple forms of ATF and AP-1.
ATF is a cellular transcription factor involved in the regulation of multiple adenovirus E1A- and cellular cAMP-inducible promoters. Using DNA affinity chromatography, we have purified ATF and found that a series of polypeptides copurify in a sequence-specific manner. We demonstrate that these polypeptides represent a family of proteins that are related by DNA-binding specificity and by immunological cross-reactivity. This family includes the transcription factor AP-1, whose recognition sequence, GTGAGTCAA, differs from the ATF consensus, GTGACGTCAA, by the absence of a cytosine residue. Our results further indicate that there are multiple forms of both ATF and AP-1. The immunological cross-reactivity and related DNA-binding specificities suggest that ATF and AP-1 contain similar amino acid sequences and may have originated from a common gene.
Hai
T W
TW
Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, Massachusetts 02138.
Liu
F
F
Allegretto
E A
EA
Karin
M
M
Green
M R
MR
D051696:Activating Transcription Factors; D001798:Blood Proteins; D002499:Centrifugation, Density Gradient; D002846:Chromatography, Affinity; D003429:Cross Reactions; D004268:DNA-Binding Proteins / Q000276:immunology* / Q000378:metabolism; D003850:Deoxyribonuclease I; D006801:Humans; D008970:Molecular Weight; D010446:Peptide Fragments / Q000276:immunology / Q000302:isolation & purification; D016755:Proto-Oncogene Proteins c-jun; D014157:Transcription Factors / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism
D051696:Activating Transcription Factors; D001798:Blood Proteins; D004268:DNA-Binding Proteins; D010446:Peptide Fragments; D016755:Proto-Oncogene Proteins c-jun; D014157:Transcription Factors; D003850:Deoxyribonuclease I