High-molecular-mass alkaline phosphatase: simplified and highly sensitive determination by liquid chromatography.
This simplified HPLC method for measurement of high-molecular-mass alkaline phosphatase (high-Mr AP; EC 3.1.3.1) in serum and bile is rapid (time for column preparation and separation 30 min), reproducible (CV 4.2%), and highly sensitive (detects high-Mr AP in healthy controls at 1-3% of total AP activity in serum), and is suitable for processing small batches of sample. We characterized high-Mr AP in serum and bile by incubating samples with L-phenylalanine, neuraminidase, 1-butanol, or wheat-germ lectin, and by determining stability to heat. High-Mr AP activity was determined in sera of patients with various liver diseases (4-32% of total AP serum activity) and results were compared with those by electrophoresis on agarose.
Schoenau
E
E
Klinik mit Poliklinik für Kinder und Jugendliche der Friedrich-Alexander Universität, Erlangen, F.R.G.
Boeswald
W
W
Herzog
K H
KH
Scheele
J
J
Boehles
H J
HJ
Stehr
K
K
D000469:Alkaline Phosphatase / Q000032:analysis* / Q000097:blood; D001646:Bile / Q000201:enzymology; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D007527:Isoenzymes / Q000032:analysis* / Q000097:blood; D008107:Liver Diseases / Q000201:enzymology; D008970:Molecular Weight
D007527:Isoenzymes; D000469:Alkaline Phosphatase