title: Comparative study of the primary structures of sero-, lacto- and ovotransferrin glycans from different species.
abstract: In order to establish relationships between glycan structure and biological activity and to answer the question: Are glycans markers of evolution?, the authors undertook a comparative study of the glycan primary structures of different transferrins (sero-, lacto- and ovotransferrins) from several species. By associating permethylation--mass spectrometry and 1H NMR spectroscopy, the primary structure of the following transferrin glycans were determined: human, bovine, hen, horse, marsupial, mouse, rabbit, rat and sheep serotransferrins; human, mouse, bovine and goat lactotransferrins; hen and turkey ovotransferrins. The results obtained led to the conclusion that transferrin glycans are specific for each transferrin and, for a given transferrin, specific to the species. No relationship could be established a priori between primary structure and function of transferrin glycans.
authors.affiliation: Université des Sciences et Techniques de Lille Flandres-Artois, Laboratoire de Chimie Biologique (Unité Associée au CNRS No. 217), Villeneuve-d'Ascq, France.
authors.forename: G
authors.initials: G
authors.lastname: Spik
authors.forename: B
authors.initials: B
authors.lastname: Coddeville
authors.forename: J
authors.initials: J
authors.lastname: Montreuil
mesh_terms: D000818:Animals; D005075:Biological Evolution; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D003207:Conalbumin; D005260:Female; D006801:Humans; D007781:Lactoferrin; D008969:Molecular Sequence Data; D015394:Molecular Structure; D011134:Polysaccharides; D013045:Species Specificity; D013329:Structure-Activity Relationship; D014168:Transferrin
chemical_list: D011134:Polysaccharides; D014168:Transferrin; D003207:Conalbumin; D007781:Lactoferrin