title: On the activities of parathyroid hormone-like factor and transforming growth factors in extract of pancreatic cancer associated with humoral hypercalcemia of malignancy.
abstract: Extract of exocrine pancreatic cancer associated with humoral hypercalcemia of malignancy was examined for biological activities of PTH-like factor and transforming growth factor (TGFA and TGFB), both of which are possible causes of hypercalcemia. The crude extract had both PTH-like and TGF activities. On Bio Gel P-60 column chromatography, PTH-like and TGFA activities were eluted at around 10 kD, whereas TGFB activity was eluted at around void fractions, 10 kD and 6 kD. Liver extract, used as a control material, exhibited only TGFB activity at around 6 kD. CM-cellulose column chromatography of 10 kD fractions resulted in a subtle distinction between PTH-like activity and TGF activities. Further fractionation of the peak with PTH-like activity on reverse-phase high performance liquid chromatography separated PTH-like activity distinctly from TGFB activity. TGFA activity was lost through the procedure. It was concluded that the exocrine pancreatic cancer associated with hypercalcemia produced not only PTH-like activity but also TGFA and TGFB activities. Several chromatographic analyses suggested that PTH-like activity and at least TGFB activity stem from distinct molecules and that the PTH-like factor has no significant TGFB activity intrinsically.
authors.affiliation: Third Department of Internal Medicine, National Defense Medical College, Saitama, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Akatsu
authors.forename: N
authors.initials: N
authors.lastname: Nagata
authors.forename: N
authors.initials: N
authors.lastname: Kugai
authors.forename: Y
authors.initials: Y
authors.lastname: Yasutomo
authors.forename: T
authors.initials: T
authors.lastname: Kinoshita
authors.forename: H
authors.initials: H
authors.lastname: Kosano
authors.forename: O
authors.initials: O
authors.lastname: Takatani
authors.forename: K
authors.initials: K
authors.lastname: Takishima
authors.forename: G
authors.initials: G
authors.lastname: Mamiya
mesh_terms: D002460:Cell Line; D002850:Chromatography, Gel; D005260:Female; D006801:Humans; D006934:Hypercalcemia / Q000378:metabolism*; D008875:Middle Aged; D010190:Pancreatic Neoplasms / Q000378:metabolism*; D010281:Parathyroid Hormone / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism*; D011869:Radioligand Assay; D015291:Transforming Growth Factors; D014410:Tumor Stem Cell Assay
chemical_list: D010281:Parathyroid Hormone; D010455:Peptides; D015291:Transforming Growth Factors