Analysis and purification of toxic peptides from cyanobacteria by reversed-phase high-performance liquid chromatography.
A simple, rapid and reliable chemical analysis method for microcystins (cyanoginosins) has been studied. Three different mobile phases for high-performance liquid chromatography were selected and optimized. Also the adsorptive powers of three commercially available C18 cartridges were compared and the results successfully applied to the clean up of three of the toxins. Finally a total system for the analysis and isolation of microcystins was established.
Harada
K
K
Faculty of Pharmacy, Meijo University, Nagoya, Japan.
Matsuura
K
K
Suzuki
M
M
Oka
H
H
Watanabe
M F
MF
Oishi
S
S
Dahlem
A M
AM
Beasley
V R
VR
Carmichael
W W
WW
D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D000458:Cyanobacteria / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D010455:Peptides / Q000032:analysis / Q000302:isolation & purification* / Q000633:toxicity; D013056:Spectrophotometry, Ultraviolet
D010455:Peptides