The effect of prostaglandin F2 alpha treatment on the endometrial oestradiol receptor system.
The effects of prostaglandin F2 alpha treatment on oestradiol receptor levels were studied in human decidual tissues from the first trimester of pregnancy. Two types of nuclear oestradiol binding sites in decidual tissue were detected. Type I receptors bind the oestradiol with high affinity and low capacity (Kd = 1.4 nM, Bmax = 0.3 pmol/mgDNA, Hillcoeff = 1), while type II binding sites have lower affinity and higher capacity for the hormone (Kd = 20.4 nM, Bmax = 2.0 pmol/mgDNA, Hillcoeff = 3). The nuclear oestrogen binding capacities of decidual tissue were affected by prostaglandin F2 alpha in a time dependent manner. Within the first 10 hrs after prostaglandin F2 alpha application the concentration of low affinity, high capacity binding sites (type II) was enhanced in comparison with the control values. Later, 20-24 hrs after the treatment, both types of nuclear oestrogen binding sites were increased significantly. When prostaglandin F2 alpha treatment failed to induce abortion no change in the concentration of nuclear oestrogen binding sites was observed. Oestradiol receptor concentration in the cytoplasm apparently was not affected by prostaglandin F2 alpha treatment.
Lànyi
E
E
Institute of Physiology, University Medical School, Pécs, Hungary.
Szèkely
J
J
Vértes
M
M
D000328:Adult; D002467:Cell Nucleus / Q000032:analysis; D003593:Cytoplasm / Q000032:analysis; D015237:Dinoprost; D004717:Endometrium / Q000032:analysis*; D005260:Female; D006801:Humans; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D011460:Prostaglandins F / Q000494:pharmacology*; D011959:Receptors, Estradiol / Q000032:analysis / Q000187:drug effects*; D011960:Receptors, Estrogen / Q000187:drug effects*
D011460:Prostaglandins F; D011959:Receptors, Estradiol; D011960:Receptors, Estrogen; D015237:Dinoprost