Heterogeneity of lysosomal enzymes in cultured normal and sialidosis type II human fibroblasts and the effect of ammonium chloride on this heterogeneity.
Lysosomal enzymes of human skin fibroblasts sediment in a Percoll gradient with major peaks at 1.05 and 1.10 g cm-3. The distribution of enzyme activity between the two peaks is dependent on growth state of the cells. Sialidosis Type II fibroblasts show a normal distribution pattern. NH4Cl stimulates enzyme secretion and de novo enzyme synthesis.
Heyworth
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Wynn
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D000643:Ammonium Chloride / Q000494:pharmacology*; D002478:Cells, Cultured; D003513:Cycloheximide / Q000494:pharmacology; D004790:Enzyme Induction / Q000187:drug effects; D005347:Fibroblasts / Q000201:enzymology; D005733:Gangliosidoses / Q000201:enzymology*; D006596:Hexosaminidases / Q000032:analysis; D006801:Humans; D008247:Lysosomes / Q000201:enzymology*; D008361:Mannosidases / Q000032:analysis; D009439:Neuraminidase / Q000172:deficiency; D043323:alpha-Mannosidase; D001616:beta-Galactosidase / Q000032:analysis / Q000172:deficiency; D001619:beta-N-Acetylhexosaminidases
D000643:Ammonium Chloride; D003513:Cycloheximide; D006596:Hexosaminidases; D008361:Mannosidases; D009439:Neuraminidase; D001616:beta-Galactosidase; D043323:alpha-Mannosidase; D001619:beta-N-Acetylhexosaminidases