The use of reverse-phase chromatographic procedures coupled with radioimmunoassay in studying regional distribution of beta h-endorphin in the human brain.
Fast reverse-phase chromatographic procedures (Sep-Pak C18 cartridges and high pressure liquid chromatography) combined with radioimmunoassay is presented. It provides accurate assessment of brain tissue concentrations of beta h-endorphin (beta h-E). The method was used to study the distribution of the endogenous opioid in nine distinct human brain regions. The beta h-E was found mainly localized in the hypothalamus anterior, hypothalamus posterior followed by a lower concentration in the Corpora mamillaria and the Substantia grisea centralis. Concentrations below 1.0 fmol/mg fresh brain tissue were determined in the Pons dorsalis, Substantia nigra, Colliculi inferiores, Glandula pinealis and in Nucleus ruber.
Pasi
A
A
Foletta
D
D
Messiha
F S
FS
Hartmann
H
H
D001923:Brain Chemistry; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004723:Endorphins / Q000032:analysis*; D006801:Humans; D011863:Radioimmunoassay; D001615:beta-Endorphin
D004723:Endorphins; D001615:beta-Endorphin