Gadolinium-labeled liposomes containing paramagnetic amphipathic agents: targeted MRI contrast agents for the liver.
Unique paramagnetic liposomal contrast agents were synthesized and utilized for selective augmentation of T1 MR imaging of the livers of normal Balb/c mice. Amphipathic gadolinium complexes, which mimic phospholipids, were incorporated into the lamella of small unilamellar liposomes (SUV) such that they became an integral part of its surface. T1 signal enhancement of normal liver approached 150% after iv administration of the paramagnetic liposomes, determined by experiments performed on a 1.9-T, experimental whole-body MRI unit. Tracer studies utilizing gadolinium-153-tagged SUV revealed that the agents exhibited excellent in vivo stability, compared to liposomal preparations in which paramagnetic agents are simply entrapped in the aqueous core of the liposome vesicle.
Kabalka
G W
GW
University of Tennessee Institute for Biomedical Imaging, Department of Radiology, Knoxville 37920.
Buonocore
E
E
Hubner
K
K
Davis
M
M
Huang
L
L
D000818:Animals; D003287:Contrast Media; D005260:Female; D019786:Gadolinium DTPA; D008081:Liposomes; D008099:Liver / Q000033:anatomy & histology*; D008279:Magnetic Resonance Imaging / Q000379:methods; D008280:Magnetics; D051379:Mice; D008807:Mice, Inbred BALB C; D009942:Organometallic Compounds; D004369:Pentetic Acid; D013501:Surface-Active Agents
D003287:Contrast Media; D008081:Liposomes; D009942:Organometallic Compounds; D013501:Surface-Active Agents; D004369:Pentetic Acid; D019786:Gadolinium DTPA