Diacylglycerol inhibits gap junctional communication in cultured epidermal cells: evidence for a role of protein kinase C.
Incubation of mouse epidermal HEL-37 cells with 1-oleoyl-2-acetylglycerol (OAG) caused a dose-dependent and transient inhibition of the transfer of microinjected fluorescein between contacting cells. Soluble extracts of HEL-37 cells contained protein kinase C activity after fractionation on DEAE-cellulose and translocation of this activity to the particulate fraction occurred after 10 min exposure to OAG or to 12-O-tetradecanoylphorbol-13- acetate (TPA). After 18 h exposure to TPA essentially all the protein kinase C activity was lost. In such TPA treated cells the transfer of microinjected fluorescein was refractory to inhibition by both TPA and OAG.
Gainer
H S
HS
Murray
A W
AW
D000818:Animals; D002450:Cell Communication / Q000187:drug effects*; D002460:Cell Line; D004075:Diglycerides / Q000494:pharmacology*; D004817:Epidermis; D019793:Fluorescein; D005452:Fluoresceins / Q000378:metabolism; D005989:Glycerides / Q000494:pharmacology*; D051379:Mice; D011493:Protein Kinase C; D047428:Protein Kinase Inhibitors; D011494:Protein Kinases / Q000502:physiology*; D013050:Spectrometry, Fluorescence; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D004075:Diglycerides; D005452:Fluoresceins; D005989:Glycerides; D047428:Protein Kinase Inhibitors; C038237:1-oleoyl-2-acetylglycerol; D011494:Protein Kinases; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate; D019793:Fluorescein