Analysis of human plasma as an exposure level monitor for carcinogenic tryptophan pyrolysis products.
A high-performance liquid chromatography method for detecting 3-amino-1,4- dimethyl-5H-pyrido[4,3-b]indole (Trp-P-1) and 3-amino-1-methyl-5H-pyrido[4,3-b]indole (Trp-P-2) in human plasma was developed. Plasma samples of 10 normal subjects were examined. Trp-P-1 and Trp-P-2, carcinogenic tryptophan pyrolysis products, were detected in all specimens, and the concentrations of Trp-P-1 and Trp-P-2 in plasma were 68.31 +/- 24.03 fmoles/ml (mean +/- S.D., n = 10) and 18.79 +/- 4.99 fmoles/ml, respectively. Our results suggest that plasma levels of carcinogenic tryptophan pyrolysis products may be useful indicators for estimating the exposure levels of the dietary carcinogens.
Manabe
S
S
Department of Hygiene and Preventive Medicine, Faculty of Medicine, University of Tokyo, Japan.
Wada
O
O
D000328:Adult; D002243:Carbolines / Q000097:blood*; D002273:Carcinogens / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D004781:Environmental Exposure; D005260:Female; D006358:Hot Temperature; D006801:Humans; D008297:Male; D013058:Mass Spectrometry; D013057:Spectrum Analysis; D014364:Tryptophan / Q000031:analogs & derivatives* / Q000097:blood
D002243:Carbolines; D002273:Carcinogens; C019133:3-amino-1-methyl-5H-pyrido(4,3-b)indole; D014364:Tryptophan; C019132:3-amino-1,4-dimethyl-5H-pyrido(4,3-b)indole