Preparation of polylactic acid-polylipoic acid nanospheres as drug targeting carriers.
Polylactic acid-polylipoic acid nanospheres (PLA-PLIA-NS) were prepared, and their properties as drug targeting dosage forms were evaluated. A computer optimization technique was applied to obtain particles with nanometer order diameters and spherical shapes. Optimum preparative conditions involved the use of 2.4% hydroxypropyl cellulose (HPC), as a stabilizing agent, 24 min ultrasonication time, and 20% PLIA content. PLIA, containing a thiol group, was used for the conjugation of antibodies with the PLA-PLIA-NS surface. In this way, fairly spherical particles of PLA-PLIA-NS with a mean diameter of about 500 nm were obtained. The conjugation was investigated to determine targeting ability more precisely. Using rabbit antihuman IgG (Fab' fragment) antibodies, conjugation with PLA-PLIA-NS was accomplished by a disulphide binding reaction. The PLA-PLIA-NS-antibody conjugates were found to recognize IgG antigen bound to the surface of Sepharose beads.
Arai
K
K
Faculty of Pharmaceutical Sciences, Hoshi University, Tokyo, Japan.
Takayama
K
K
Nambu
Y
Y
Nambu
N
N
Ueda
H
H
Machida
Y
Y
Nagai
T
T
D004337:Drug Carriers; D004655:Emulsions; D007074:Immunoglobulin G; D007700:Kinetics; D007773:Lactates / Q000138:chemical synthesis*; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D008968:Molecular Conformation; D011108:Polymers / Q000138:chemical synthesis*; D011239:Prednisolone / Q000008:administration & dosage; D008063:Thioctic Acid / Q000031:analogs & derivatives* / Q000138:chemical synthesis
D004337:Drug Carriers; D004655:Emulsions; D007074:Immunoglobulin G; D007773:Lactates; D011108:Polymers; C060808:polylactic acid-polylipoic acid; D008063:Thioctic Acid; D011239:Prednisolone