The major histocompatibility complex of the cynomolgus monkey. II. Polymorphism at three serologically defined loci and correlation of haplotypes with stimulation in MLC and skin graft survival.
The major histocompatibility complex (MHC) of the cynomolgus monkey has been further characterized by testing in population and family studies 50 new alloantisera along with previously characterized sera. This resulted in the redefinition of three of the original 21 CyLA specificities and the identification of 14 additional specificities. Genetic evidence for the existence of a third class I locus, designated CyLa-C, is presented together with evidence for the linkage of a locus-controlling allostimulation of mixed lymphocyte cultures (MLC) to this complex. The number of alleles assigned to the CyLA-A locus was increased from seven to 14, the B locus from six to ten, and the newly defined C locus was assigned six alleles. Haplotype and gene frequencies were counted from the family data and compared with estimates from the population. Several examples of linkage disequilibrium were noted, though this does not appear to be a pronounced feature of the system. One informative family was identified in which a recombination resulted in the segregation of the MLC response with CyLA-A thus, implying that this may be the homologue of the B locus of other primate systems. In addition to the linkage of an MLC locus to CyLA, further evidence that this is the cynomolgus MHC came from the effect of haplotype matching on the survival of skin grafts exchanged within families. An increase in the mean graft survival time was dependent upon the number of haplotypes matched. The organization and degree of polymorphism of the cynomolgus MHC was shown to be homologous to other primate systems, which should allow this species to serve as a substitute for the rhesus macaque as a genetically defined model in transplantation and immunology research.
Keever
C A
CA
Heise
E R
ER
D000483:Alleles; D000818:Animals; D005260:Female; D006085:Graft Survival; D066298:In Vitro Techniques; D007959:Lymphocyte Culture Test, Mixed; D008251:Macaca / Q000235:genetics*; D008252:Macaca fascicularis / Q000235:genetics* / Q000276:immunology; D008285:Major Histocompatibility Complex; D008297:Male; D011110:Polymorphism, Genetic; D016038:Skin Transplantation; D014184:Transplantation, Homologous