Ultrastructural localization of antigens recognized by the monoclonal antibodies MB1, MB2, MT1, UCHL1, and TAL 1B5.
In this study the ultrastructural localization of antigens recognized by novel antibodies that allow the recognition of lymphoid antigens in conventionally fixed and wax-embedded sections was investigated. MT1, MB1, and UCHL1 recognize antibodies restricted to the cell membrane, whereas the antigen recognized by MB2 is present only in the cytoplasm. These distributions are different from that of immunoreactivity with TAL 1B5 (anti-HLA DR), which is present both on the cell membrane and in the cytoplasm. The significance of these findings is discussed.
Butler
M G
MG
Department of Histopathology, St. Bartholomew's Hospital, London, United Kingdom.
Wells
C
C
d'Ardenne
A J
AJ
D000911:Antibodies, Monoclonal / Q000032:analysis / Q000276:immunology*; D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure; D003593:Cytoplasm / Q000378:metabolism / Q000648:ultrastructure; D006680:HLA Antigens / Q000032:analysis* / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000032:analysis / Q000276:immunology; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D014066:Palatine Tonsil / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure
D000911:Antibodies, Monoclonal; D006680:HLA Antigens; D000949:Histocompatibility Antigens Class II