Phosphatidylinositol metabolism and protein kinase C activation in leukocytes by lipopeptides.
The synthetic lipopeptide Pam3Cys-Ser-Ser-Asn-Ala, an analogue of the N-terminal part of bacterial lipoprotein, constitutes a potent macrophage and B lymphocyte activator. In the macrophage cell-line P388D1 Pam3Cys-Ser-Ser-Asn-Ala stimulated phosphoinositol turnover, whereas in small resting lymphocytes no enhanced turnover was observed. Upon lipopeptide stimulation, a translocation of PKC from the cytosol to the plasma membrane was found in the cell line P388D1 but not in lymphocytes. Substituting lipopeptide for diacylglycerol shows that Pam3Cys-Ser-Ser-Asn-Ala leads to an activation of protein kinase C at Ca2+ concentrations of 0.5 mM. Thus, mitogenic lipopeptides constitute novel tools for investigating the molecular mechanism of transmembrane signaling in leukocyte activation.
Hauschildt
S
S
Institut für Immunbiologie, Universität Freiburg, Federal Republic of Germany.
Wagner-Roos
L M
LM
Wiesmüller
K H
KH
Jung
G
G
Bessler
W G
WG
D000818:Animals; D002460:Cell Line; D004789:Enzyme Activation / Q000187:drug effects; D066298:In Vitro Techniques; D007962:Leukocytes / Q000187:drug effects* / Q000378:metabolism; D055666:Lipopeptides; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008934:Mitogens / Q000494:pharmacology; D009842:Oligopeptides / Q000494:pharmacology*; D010716:Phosphatidylinositols / Q000378:metabolism*; D011493:Protein Kinase C / Q000378:metabolism*
D055666:Lipopeptides; D008934:Mitogens; D009842:Oligopeptides; D010716:Phosphatidylinositols; C038293:2,3-bis(palmitoyloxy)-2-propyl-N-palmitoyl-cysteinyl-seryl-seryl-asparaginyl-alanine; C041546:N-palmitoyl-seryl-seryl-asparaginyl-alanine; D011493:Protein Kinase C