Retinoic acid-induced differentiation of retrovirus-infected HL-60 cells is associated with enhanced transcription from the viral long terminal repeat.
I infected different human leukemic cell lines with an amphotropic retrovirus vector (designated PA317/N2) which confers G418 resistance and contains the Moloney murine leukemia virus long terminal repeat. In retrovirus-infected G418-resistant HL-60 cells, induction of granulocyte differentiation by retinoic acid was invariably accompanied by a marked increase (5- to 10-fold) in the transcriptional activity of the integrated retroviral long terminal repeat.
Collins
S J
SJ
Molecular Medicine Program, Fred Hutchinson Cancer Research Center, Seattle, Washington 98104.
D015152:Blotting, Northern; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D004351:Drug Resistance / Q000235:genetics; D005786:Gene Expression Regulation; D005814:Genes, Viral; D005822:Genetic Vectors; D005839:Gentamicins; D006801:Humans; D008979:Moloney murine leukemia virus / Q000235:genetics; D012190:Retroviridae / Q000235:genetics*; D014158:Transcription, Genetic; D014212:Tretinoin / Q000494:pharmacology
D005839:Gentamicins; D014212:Tretinoin; C010680:antibiotic G 418