Genes controlling malathion resistance in a laboratory-selected population of Drosophila melanogaster.
The chromosomal locations of several genes responsible for increased malathion resistance in a laboratory-selected population of Drosophila melanogaster have been determined. These genes appear to be involved in the regulation of microsomal cytochrome P-450. A major gene on chromosome 2 (2-64) and at least two genes on chromosome 3 (near 3-58) control increased mixed function oxidase activity, and both larval and adult malathion resistance. Although the chromosome 2 locus was not associated with a significant increase in cytochrome P-450 content, SDS polyacrylamide gel electrophoresis of microsomal proteins detected increased silver staining of a polypeptide having a relative molecular mass (Mr) of about 52,000. Microsomes from strains carrying the chromosome 3 factors for resistance contained more cytochrome P-450 and increased amounts of two heme-staining protein bands (Mr = 50,000 and 54,000). The genes regulating these proteins were closely linked to striped at 3-62 and probably identical to the loci responsible for malathion resistance and increased mixed function oxidase activity. Other R genes on both chromosomes 2 and 3 as well as target resistance were required for the full expression of malathion resistance in the selected Drosophila population. Exposure of this Drosophila melanogaster population to malathion selected a polygenic system for the oxidative metabolism of insecticide.
Houpt
D R
DR
Department of Biology, McMaster University, Hamilton, Ont., Canada.
Pursey
J C
JC
Morton
R A
RA
D000818:Animals; D002874:Chromosome Mapping; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics; D004331:Drosophila melanogaster / Q000235:genetics* / Q000378:metabolism; D004351:Drug Resistance / Q000235:genetics*; D008294:Malathion; D008861:Microsomes / Q000378:metabolism; D006899:Mixed Function Oxygenases / Q000235:genetics; D011506:Proteins / Q000235:genetics
D011506:Proteins; D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases; D008294:Malathion