title: Xenogenization of tumor cells by transfection with plasmid containing env gene of Friend leukemia virus.
abstract: A rat hepatocellular carcinoma cell line (cKDH-8 cl-11) showed decreased tumorigenicity after transfection with an envelope gene derived from a Friend leukemia virus (FV-env gene). FV-env gene product was found by indirect immunofluorescence staining to be expressed on the cell surface of the FV-env gene-transfected cells. The FV-env-transfected cells (FV-env cKDH-8), however, grew well in X-irradiated immunosuppressed rats, indicating that the reduction in tumorigenicity of the transfected cells is based on immunological reaction in the host. The rats which rejected FV-env cKDH-8 cells showed resistance to rechallenge with the parent cKDH-8 cl-11 tumor cells. These results suggest that the FV-env gene product may elicit antitumor immunity against FV-env cKDH-8 cells in a host with a resultant reduction in the tumorigenicity of these cells.
authors.affiliation: Laboratory of Pathology, Hokkaido University School of Medicine, Sapporo.
authors.forename: C
authors.initials: C
authors.lastname: Sugiura
authors.forename: T
authors.initials: T
authors.lastname: Itaya
authors.forename: N
authors.initials: N
authors.lastname: Kondoh
authors.forename: T
authors.initials: T
authors.lastname: Oikawa
authors.forename: N
authors.initials: N
authors.lastname: Kuzumaki
authors.forename: N
authors.initials: N
authors.lastname: Takeichi
authors.forename: M
authors.initials: M
authors.lastname: Hosokawa
authors.forename: H
authors.initials: H
authors.lastname: Kobayashi
mesh_terms: D000818:Animals; D005622:Friend murine leukemia virus / Q000235:genetics*; D005814:Genes, Viral; D008114:Liver Neoplasms, Experimental / Q000276:immunology*; D009368:Neoplasm Transplantation; D010957:Plasmids; D051381:Rats; D011919:Rats, Inbred Strains; D014162:Transfection; D014407:Tumor Cells, Cultured; D014759:Viral Envelope Proteins / Q000235:genetics*
chemical_list: D014759:Viral Envelope Proteins