[Comparative study of ELISA and RIA technics for the detection of specific IgG4].
The assay of IgG4 was done by an ELISA method that has been perfected in our laboratory over two years, or by the RIA (Pharmacia technique). Assay of IgG4 is an objective element in the control of specific immunotherapy (IT) to hymenoptera venoms and grass pollens. There is an excellent correlation between the two techniques of ELISA and RIA, that shows an increase in IgG4 titre after around 3 to 4 years of IT.
Sabbah
A
A
Laboratoire d'Immuno-Allergologie CHU, Angers.
Le Dauphin
C
C
Heulin
M G
MG
D000918:Antibody Specificity; D001514:Bee Venoms / Q000627:therapeutic use; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis*; D007299:Insect Bites and Stings / Q000628:therapy; D011058:Pollen / Q000276:immunology; D011863:Radioimmunoassay; D006255:Rhinitis, Allergic, Seasonal / Q000628:therapy; D014862:Wasp Venoms / Q000627:therapeutic use
D001514:Bee Venoms; D007074:Immunoglobulin G; D014862:Wasp Venoms
Etude comparative des méthodes ELISA et RIA pour le dosage des IgG4 spécifiques.