H-2 restriction and H-2 phenotypes of spleen cells from thymus-grafted radiation chimeras: evidence consistent with extrathymic suppression.
As an approach to analyzing the factors that contribute to determining H-2 restriction specificities of cytotoxic T cells, thymectomized semiallogeneic radiation chimeras were given transplants of fetal thymus from parental strain or F1 hybrid donors. A pronounced preference for lysis of infected targets of the same H-2 haplotype as the thymus was observed, the bias ranging from an undetectable preference through to absolute restriction to the thymic H-2. Virus-immune Tc cells could also be restricted to H-2 antigenic determinants of haplotypes expressed only by donated stem cell progeny lymphomyeloid cells. T cells from chimeras that had received thymuses of both parental strains were less effective than normal syngeneic F1 hybrid T cells in lysing allogeneic cells and both infected and uninfected parental targets. Variation in H-2 restriction bias from one chimera to another was not reflected in modulations to the class I H-2 phenotypes of the chimeric spleen cells.
Gallagher
P F
PF
Department of Microbiology, John Curtin School of Medical Research, Australian National University, Canberra.
Ashman
R B
RB
Blanden
R V
RV
D000818:Animals; D004481:Ectromelia virus / Q000276:immunology; D006183:H-2 Antigens / Q000235:genetics / Q000276:immunology*; D007108:Immune Tolerance; D007114:Immunization; D051379:Mice; D008815:Mice, Inbred Strains / Q000235:genetics / Q000276:immunology; D010641:Phenotype; D011828:Radiation Chimera / Q000235:genetics / Q000276:immunology*; D011948:Receptors, Antigen, T-Cell / Q000235:genetics; D013154:Spleen / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D013950:Thymus Gland / Q000276:immunology* / Q000637:transplantation
D006183:H-2 Antigens; D011948:Receptors, Antigen, T-Cell