title: Multiple ionic forms of ornithine decarboxylase differ in degree of phosphorylation.
abstract: Two major ionic forms of ornithine decarboxylase were separated by column chromatography of extracts of kidneys from androgen-treated male CD-1 mice on DEAE-Sepharose CL-6B, and purified individually to apparent homogeneity. On SDS-PAGE, a single major protein band of Mr 50000 was present in each. When incubated with casein kinase II, purified from rat liver cytosol, only one form of the enzyme, which represented 20% of the total ornithine decarboxylase in the tissue, became phosphorylated. The major form, which was eluted later from the column, could be phosphorylated only after treatment with alkaline phosphatase, indicating that the phosphatase removed enzyme-bound phosphate already attached at the casein kinase II phosphorylation site. Evidence for the occurrence of a phosphorylated form of the enzyme in kidneys of dexamethasone-treated rats is also presented.
authors.affiliation: Department of Biochemistry, University of British Columbia, Vancouver, Canada.
authors.forename: T
authors.initials: T
authors.lastname: Peng
authors.forename: J F
authors.initials: JF
authors.lastname: Richards
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D047388:Casein Kinases; D003907:Dexamethasone / Q000494:pharmacology; D007527:Isoenzymes / Q000378:metabolism*; D007668:Kidney / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D008297:Male; D051379:Mice; D008970:Molecular Weight; D009955:Ornithine Decarboxylase / Q000378:metabolism*; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism
chemical_list: D007527:Isoenzymes; D003907:Dexamethasone; D011494:Protein Kinases; D047388:Casein Kinases; D000469:Alkaline Phosphatase; D009955:Ornithine Decarboxylase