title: Regulation by Ca2+ of membrane elasticity of bovine chromaffin granules.
abstract: In a range of [Ca2+] similar to cytosolic transient, a drastic reduction from about 20 dyn/cm to almost zero was observed in the membrane elastic modulus of bovine chromaffin granules, isolated in a solution containing 0.3 M sucrose and 5 mM Hepes at pH 7.0, and measured by combination of osmotic swelling and dynamic light-scattering (DLS) methods. This result suggests that the granule membrane becomes extremely flexible as a prelude to exocytosis.
authors.affiliation: Mitsubishi Kasei Institute of Life Sciences, Tokyo, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Miyamoto
authors.forename: S
authors.initials: S
authors.lastname: Fujime
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002417:Cattle; D002837:Chromaffin Granules / Q000378:metabolism* / Q000648:ultrastructure; D002838:Chromaffin System / Q000378:metabolism*; D004548:Elasticity; D007425:Intracellular Membranes / Q000502:physiology* / Q000648:ultrastructure; D008027:Light; D008962:Models, Theoretical; D009994:Osmolar Concentration; D012542:Scattering, Radiation
chemical_list: D002118:Calcium