Distribution of Thyroliberin (TRH)- and 12-O-tetradecanoylphorbol 13-acetate (TPA)-activated adenylyl cyclase in normal and neoplastic tissue with special reference to the prostate.
Tissue specificity of the Thyroliberin (TRH)- and 12-O-tetradecanoylphorbol 13-acetate (TPA)-sensitive adenylyl cyclase has been studied using normal or neoplastic organ samples or cells from the pituitary gland, stomach, prostate, myocardium, liver and bone. It appeared that TRH stimulates the adenylyl cyclase in both normal (basal cells), hyperplastic and adenocarcinomatous prostate as well as in the pituitary and stomach. TPA also stimulated the enzyme from the prostate and other organs/cells, but to a greater extent in neoplastic tissue. Functional links from protein kinase C to adenylyl cyclase and from protein kinase C to tyrosine kinase/oncogene expression have been established. Hence it is believed that TRH, which stimulates the adenylyl cyclase and protein kinase C in the pituitary, may serve as a factor contributing to transformation of prostatic cells or enhanced cell proliferation in prostatic cancer.
Gordeladze
J O
JO
Institute of Surgical Research, National Hospital, Rikshopitalet, Oslo, Norway.
Rur
H
H
Attramadal
A
A
Tveter
K
K
Gautvik
K M
KM
Purvis
K
K
D000262:Adenylyl Cyclases / Q000032:analysis*; D000818:Animals; D006801:Humans; D008297:Male; D010703:Phorbol Esters / Q000494:pharmacology; D010902:Pituitary Gland / Q000201:enzymology; D011467:Prostate / Q000201:enzymology*; D011471:Prostatic Neoplasms / Q000201:enzymology*; D051381:Rats; D013270:Stomach / Q000201:enzymology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D013973:Thyrotropin-Releasing Hormone / Q000032:analysis*
D010703:Phorbol Esters; D013973:Thyrotropin-Releasing Hormone; D000262:Adenylyl Cyclases; D013755:Tetradecanoylphorbol Acetate