Experimental infections of simians with human malaria: attempts to infect Callithrix penicillata with Plasmodium falciparum.
After reviewing the use of non-human primates of the Old and New Worlds for human malaria research, we concluded that another experimental animal which is easily available to use and possible to rear indoors is needed. Thus, we studied the susceptibility of the marmoset Callithrix penicillata to Plasmodium falciparum erythrocytic infections. The marmosets received various P. falciparum human isolates, directly from a patient and from continuous cultures. The Palo Alto strain, which has been adapted to the night monkey Aotus trivirgatus and further maintained in the squirrel monkey Saimiri sciureus was also used. In a total of 20 marmosets we performed 31 inoculations, with 10(5) to 10(9) parasites, intraperitoneally, intracardiacly or intravenously. Blood samples from each animal were examined daily up to day 90 post-inoculation. None of the intact marmosets developed patent infections. Four out of 19 C. penicillata, previously splenectomized, showed circulating parasites for up to five days after intravenous inoculation with the Palo Alto strain, becoming negative thereafter. Neither the addition to the simian diet of p-aminobenzoic acid, essential for the parasite metabolism, nor drug-immunosuppression, improved the marmoset susceptibility to P. falciparum.
da Rocha
E M
EM
Department of Parasitology, University of Minas Gerais, Brazil.
Pereira
L H
LH
Do Rosario
V E
VE
Krettli
A U
AU
D000818:Animals; D002144:Callithrix; D002143:Callitrichinae; D004198:Disease Susceptibility; D006801:Humans; D008288:Malaria / Q000635:transmission*; D010963:Plasmodium falciparum