title: Localization of the 68,000-Da human neurofilament gene (NF68) using a murine cDNA probe.
abstract: A recent investigation, using a human genomic probe, has indicated that the 68,000 dalton neurofilament gene (NF68) is on the short arm of chromosome 8. We have used a murine cDNA probe on 65 metaphase spreads in situ to localize the human NF68 gene to 8p21 (20/370 grains; p less than 0.0001). In addition, we have found secondary hybridization sites at the centromeric region of chromosome 2 and the long arm of chromosome 7, which are putative loci for other intermediate filaments.
authors.affiliation: Department of Physiology, University of Toronto, Ont., Canada.
authors.forename: M J
authors.initials: MJ
authors.lastname: Somerville
authors.forename: D R
authors.initials: DR
authors.lastname: McLachlan
authors.forename: M E
authors.initials: ME
authors.lastname: Percy
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism; D002871:Chromosome Banding; D002874:Chromosome Mapping; D002898:Chromosomes, Human, Pair 8; D004247:DNA / Q000235:genetics; D005796:Genes; D006801:Humans; D007381:Intermediate Filament Proteins / Q000235:genetics*; D007621:Karyotyping; D051379:Mice; D008970:Molecular Weight; D016900:Neurofilament Proteins; D013601:T-Lymphocytes / Q000166:cytology
chemical_list: D007381:Intermediate Filament Proteins; D016900:Neurofilament Proteins; D004247:DNA