The composition of the ceramides from human stratum corneum and from comedones.
Human epidermal surface lipids were collected by an ethanol wash and the ceramides were quantified by thin-layer chromatography-photodensitometry. Six ceramide fractions were isolated and the structural components of each were analyzed in detail. The most unusual of the epidermal ceramides contained a sphingosine base with amide-linked 30- and 32-carbon omega-hydroxyacids and an ester-linked nonhydroxyacid, 41% of which was linoleic acid. The proportion of linoleic acid in the analogous ceramide from comedones was 6%. This supports the hypothesis that a localized insufficiency of linoleic acid in the follicular epithelium is an etiologic factor in comedogenesis.
Wertz
P W
PW
Miethke
M C
MC
Long
S A
SA
Strauss
J S
JS
Downing
D T
DT
D000152:Acne Vulgaris / Q000378:metabolism*; D000328:Adult; D000818:Animals; D002518:Ceramides / Q000032:analysis*; D002855:Chromatography, Thin Layer; D004817:Epidermis / Q000032:analysis*; D005227:Fatty Acids / Q000032:analysis; D006801:Humans; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000032:analysis; D008297:Male; D013110:Sphingosine / Q000032:analysis; D013552:Swine
D002518:Ceramides; D005227:Fatty Acids; D008041:Linoleic Acids; D019787:Linoleic Acid; D013110:Sphingosine