Effect of streptozotocin-induced diabetes on the activity of 7 alpha-hydroxy-4-cholesten-3-one-specific 12 alpha-hydroxylase in rats.
The activity of 12 alpha-hydroxylase in hepatic microsomes from normal, streptozotocin-induced diabetic, and insulin-treated diabetic rats was studied with 7 alpha-hydroxy-4-cholesten-3-one as a substrate. In the diabetic rats, the 12 alpha-hydroxylase activity was about 50% lower than in the normal rats. Treatment of the diabetics with insulin cancelled the reduction of the activity. These results show that an insulin-deficient state causes a paradoxical decrease in the activity of the key enzyme for cholic acid formation.
Kimura
K
K
Department of Biochemistry, Tottori University School of Medicine.
Ogura
M
M
D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy / Q000201:enzymology*; D007328:Insulin / Q000627:therapeutic use; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology*; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values; D013253:Steroid 12-alpha-Hydroxylase / Q000378:metabolism*; D013250:Steroid Hydroxylases / Q000378:metabolism*
D007328:Insulin; D003577:Cytochrome P-450 Enzyme System; D013250:Steroid Hydroxylases; C035248:7 alpha-hydroxy-4-cholesten-3-one-12 alpha monooxygenase; D013253:Steroid 12-alpha-Hydroxylase