title: Escherichia coli heat shock gene mutants are defective in proteolysis.
abstract: Heat shock proteins in Escherichia coli are relatively abundant and some are essential for growth, but the function that they provide is unknown. The observation that heat shock proteins are induced by some abnormal, rapidly degraded polypeptides, and that strains with mutations in the rpoH gene, the positive regulator of heat shock gene expression, are defective in proteolysis, has led to the proposal that heat shock proteins are required for normal degradation of polypeptides. We have investigated this hypothesis by examining the degradation of polypeptide fragments generated by puromycin and the degradation of a nonsense fragment of beta-galactosidase. Mutations in the dnaK, dnaJ, grpE, and groEL heat shock genes result in defective proteolysis. Furthermore, overproduction of heat shock proteins results in enhanced rates of puromycyl fragment decay. The proteolysis defect of the heat shock gene mutants primarily affects energy-dependent protein degradation. These results indicate that at least one general function of heat shock proteins is to contribute to the ability of the cell to degrade abnormal polypeptides.
authors.affiliation: Department of Bacteriology, University of Wisconsin, Madison 53706.
authors.forename: D B
authors.initials: DB
authors.lastname: Straus
authors.forename: W A
authors.initials: WA
authors.lastname: Walter
authors.forename: C A
authors.initials: CA
authors.lastname: Gross
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D004734:Energy Metabolism; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000235:genetics* / Q000378:metabolism; D006868:Hydrolysis; D009154:Mutation; D010446:Peptide Fragments / Q000378:metabolism; D011691:Puromycin / Q000494:pharmacology; D001616:beta-Galactosidase / Q000235:genetics
chemical_list: D006360:Heat-Shock Proteins; D010446:Peptide Fragments; D011691:Puromycin; D000255:Adenosine Triphosphate; D001616:beta-Galactosidase