Effects of 1,1,1-trichloro-2,2-bis-(p-chlorophenyl)ethane (DDT) on ATPase-linked functions in isolated rat-liver mitochondria.
The effects of DDT on the energy-related functions of rat-liver mitochondria were examined. ADP-stimulated respiration was much more sensitive to inhibition by DDT than was uncoupler-stimulated respiration when succinate or ascorbate/TMPD was used as the substrate. Ca2+ uptake driven by ATP hydrolysis was inhibited by DDT. These results indicate that DDT inhibits ATPase itself. In addition, DDT blocked succinate dehydrogenase and the cytochrome b-c span of the electron transport chain, which also secondarily reduced ATP synthesis. The uncoupling action due to DDT was only seen at high concentrations with ascorbate/TMPD as the substrate. However, this action was masked because of the increased inhibition of the electron transport chain when the substrate was changed to succinate.
Nishihara
Y
Y
Utsumi
K
K
D019297:2,4-Dinitrophenol; D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors*; D000818:Animals; D002118:Calcium / Q000378:metabolism; D003634:DDT / Q000633:toxicity*; D004140:Dinitrophenols / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D066298:In Vitro Techniques; D008297:Male; D008930:Mitochondria, Liver / Q000187:drug effects / Q000201:enzymology*; D010085:Oxidative Phosphorylation / Q000187:drug effects; D010101:Oxygen Consumption / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains
D004140:Dinitrophenols; D003634:DDT; D000251:Adenosine Triphosphatases; D019297:2,4-Dinitrophenol; D002118:Calcium