Genes on chromosomes 1 and 4 in the mouse are associated with repair of radiation-induced chromatin damage.
Early-passage skin fibroblasts from different inbred and congenic strains of mice were X-irradiated (1 Gy), and the number of chromatid breaks was determined at 2.0 h after irradiation. The cells from DBA/2N, C3H/HeN, STS/A, C57BL/6N, BALB/cJ, and AKR/N had 25 to 42 chromatid breaks per 100 metaphase cells (efficient repair phenotype). NZB/NJ had greater than 78 and BALB/cAn had 87 to 110 chromatid breaks per 100 cells (inefficient repair phenotype). Differences between BALB/cAn and BALB/c. DBA/2 congenic strains which carry less than 1% of the DBA/2 genome indicate that two genes, one on chromosome 1 linked to bcl-2-Pep-3 and the other on chromosome 4 closely linked to Fv-1, affect the efficiency with which the cells repair radiation-induced chromatin damage.
Potter
M
M
Laboratory of Genetics, National Cancer Institute, Bethesda, Maryland 20892.
Sanford
K K
KK
Parshad
R
R
Tarone
R E
RE
Price
F M
FM
Mock
B
B
Huppi
K
K
D000818:Animals; D002478:Cells, Cultured; D002843:Chromatin / Q000528:radiation effects*; D004260:DNA Repair; D005347:Fibroblasts / Q000528:radiation effects; D008040:Genetic Linkage; D005819:Genetic Markers; D051379:Mice; D008815:Mice, Inbred Strains; D012867:Skin / Q000166:cytology / Q000528:radiation effects
D002843:Chromatin; D005819:Genetic Markers