Networks of DNA and RecA protein are intermediates in homologous pairing.
Partial coating of single-stranded DNA by recA protein causes its aggregation, but conditions that promote complete coating inhibit independent aggregation of single strands and, instead, cause the mutually dependent conjunction of single- and double-stranded DNA in complexes that sediment at more than 10 000 S. This coaggregation is independent of homology but otherwise shares key properties of homologous pairing of single strands with duplex DNA: both processes require ATP, MgCl2, and stoichiometric amounts of recA protein; both are very sensitive to inhibition by salt and ADP. Coaggregates are closed domains that are intermediates in homologous pairing: they form faster than joint molecules, they include virtually all of the DNA in the reaction mixture, and they yield joint molecules nearly an order of magnitude faster than they exchange DNA molecules with the surrounding solution. The independent aggregation of single-stranded DNA differs in all respects except the requirement for Mg2+, and its properties correlate instead with those associated with the renaturation of complementary single strands by recA protein.
Tsang
S S
SS
Chow
S A
SA
Radding
C M
CM
D010584:Bacteriophage phi X 174; D003090:Coliphages; D004270:DNA, Circular / Q000378:metabolism; D004277:DNA, Single-Stranded / Q000378:metabolism*; D004279:DNA, Viral / Q000378:metabolism*; D007700:Kinetics; D046911:Macromolecular Substances; D009692:Nucleic Acid Heteroduplexes / Q000378:metabolism*; D009994:Osmolar Concentration; D011938:Rec A Recombinases / Q000378:metabolism*; D012965:Sodium Chloride / Q000494:pharmacology
D004270:DNA, Circular; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D046911:Macromolecular Substances; D009692:Nucleic Acid Heteroduplexes; D012965:Sodium Chloride; D011938:Rec A Recombinases