31P and 1H magnetic resonance spectroscopy of acute alcohol cardiac depression in rats.
Cardiac depression in the isolated rat heart perfused with 4% ethanol was correlated with intracellular phosphate energetics and tissue water distributions. Energy metabolites were assessed using 31P magnetic resonance spectroscopy (MRS) and correlated to the mitochondrial redox state using epicardial surface fluorometry. Changes in myocardial water compartmentation were measured by using 1H NMR spectroscopy with an extracellular chemical-shift reagent (DyTTHA) and correlated to results of 2D echocardiography (2DE). During alcohol perfusion there was a significant decrease in developed pressure and in coronary flow. No change was seen in ATP, PCr, pHi, Pi, or NADH. After withdrawal of alcohol from the perfusate cardiac function reverted to control values without a depletion of energy levels. During alcohol perfusion 1H MRS showed a marked redistribution of water from the intra- to the extracellular space, corresponding to a 35% left ventricular wall thinning confirmed by 2DE. The results indicate that acute alcohol cardiac depression is related to a dehydration of myocardial cells, but is not associated with intracellular acidosis or energy depletion.
Auffermann
W
W
Department of Radiology, University of California, San Francisco 94143.
Camacho
S A
SA
Wu
S
S
Litt
L
L
Parmley
W W
WW
Higgins
C B
CB
Wikman-Coffelt
J
J
D000208:Acute Disease; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003326:Coronary Circulation; D003864:Depression, Chemical; D004452:Echocardiography; D000431:Ethanol / Q000633:toxicity*; D005470:Fluorometry; D006321:Heart / Q000187:drug effects* / Q000503:physiopathology; D006859:Hydrogen; D066298:In Vitro Techniques; D009682:Magnetic Resonance Spectroscopy; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology; D010758:Phosphorus; D051381:Rats
D010758:Phosphorus; D000431:Ethanol; D006859:Hydrogen