Preparation of monoclonal antibodies to chicken bone phosphoproteins.
Monoclonal and polyclonal antibodies were raised against 150k, 60k, 32k, and 15k phosphoprotein components of 14-week chicken bone. Hybridomas were prepared by immunizing Balb/c mice with 150k, 60k, or 32k phosphoproteins followed by fusion of their spleen cells with X63-Ag8.653 myeloma cells. Polyclonal antibodies to the 60k, 32k, and 15k phosphoprotein components were produced in immunized rabbits. Immunological cross-reactivity between antigen and antibody was examined by enzyme-linked immunosorbent assay (ELISA) and dot-blotting. There was cross-reactivity between the 150k, 32k, and 15k phosphoprotein components and between the 150k, 90k, and 60k components. The antibodies raised against 60k component do not bind 32k and 15k antigens.
Yamazaki
K
K
Department of Orthopedic Surgery, Children's Hospital, Boston, Massachusetts.
Suzuki
M
M
Mikuni-Takagaki
Y
Y
Hiraiwa
K
K
Lefteriou
B
B
Glimcher
M J
MJ
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D001842:Bone and Bones / Q000032:analysis*; D002645:Chickens; D002848:Chromatography, DEAE-Cellulose; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D010750:Phosphoproteins / Q000032:analysis / Q000276:immunology*
D000911:Antibodies, Monoclonal; D010750:Phosphoproteins