title: Pseudomonas aeruginosa acid phosphatase contains an anionic site with a trimethyl subsite. Kinetic evidences obtained with alkylammonium ions.
abstract: In this work the action of the following compounds upon Ps. aeruginosa acid phosphatase has been studied: 1) alkylammonium compounds; 2) aminoalcohols and aminoacids with different substituents (-H, -CH2OH and -CH3) attached to the nitrogen atom; 3) alcohols analogous to some compounds of the above series, but without the amino group. It was found that the enzyme inhibition was more effective with N-trimethylated compounds than with the triethylated ones. The degree of inhibition depended on the number of methyl groups bound to the nitrogen atom. Taking into account the choline and betaine series the hydroxyl derivatives showed more affinity for the enzyme than the carboxylated ones. In each series the Ki values increased with the decrease of methyl groups bound to the nitrogen atom. The presence of a positively charged nitrogen atom in the molecule of the effector was essential. These results enable us to confirm that in the molecule of Ps. aeruginosa acid phosphatase there exists an anionic site with one subsite with affinity for methyl groups.
authors.affiliation: Departamento de Biología Molecular, Facultad de Ciencias Exactas, Universidad Nacional de Río Cuarto, Córdoba, Argentina.
authors.forename: M N
authors.initials: MN
authors.lastname: Garrido
authors.forename: T A
authors.initials: TA
authors.lastname: Lisa
authors.forename: C E
authors.initials: CE
authors.lastname: Domenech
mesh_terms: D000135:Acid Phosphatase / Q000032:analysis* / Q000037:antagonists & inhibitors; D000838:Anions; D001665:Binding Sites; D007700:Kinetics; D011550:Pseudomonas aeruginosa / Q000201:enzymology*; D000644:Quaternary Ammonium Compounds
chemical_list: D000838:Anions; D000644:Quaternary Ammonium Compounds; D000135:Acid Phosphatase