Generation of transducing particles in Staphylococcus aureus.
Transduction of plasmid pC194 and bacteriophage phi 11de varied inversely with the multiplicity of infection. As the multiplicity of infection decreased from 10(-1) to 10(-5) PFU/CFU, the transduction frequency of pC194 increased 10(4)-fold; the transduction frequency of phi 11de increased 300-fold with a 100-fold decrease in multiplicity of infection. Physical and genetic analysis of the transduced DNA showed that pC194 resided in the phage particle as a random, circularly permuted linear concatemer. In DNA prepared from phage that cotransduced pC194 and phi 11de, pC194 resided in the transducing phage primarily as a linear multimer of 15.8 kilobases, or about 5.4 pC194 monomers. The pC194 multimer was randomly inserted into the phi 11 genome.
Dyer
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DW
Rock
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MI
Lee
C Y
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Iandolo
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JJ
D004279:DNA, Viral; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D013204:Staphylococcus Phages / Q000235:genetics / Q000528:radiation effects; D013211:Staphylococcus aureus / Q000235:genetics* / Q000648:ultrastructure; D014161:Transduction, Genetic; D014466:Ultraviolet Rays
D004279:DNA, Viral