Synergistic effect of dosage and bacterial inoculum in TEM-1 mediated antibiotic resistance.
The effect of inoculum size and gene dosage on the level of antibiotic resistance mediated by TEM-1 beta-lactamase was measured. From the results it seemed that gene dosage is a more efficient mechanism than inoculum size for increasing TEM-1 mediated resistance to beta-lactam antibiotics. It also seemed that the two mechanisms for enhancing antibiotic resistance are synergistic. The clinical implications of these results are discussed.
Reguera
J A
JA
Servicio de Microbiologia, Hospital Ramon y Cajal, Madrid, Spain.
Baquero
F
F
Perez-Diaz
J C
JC
Martinez
J L
JL
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D004352:Drug Resistance, Microbial / Q000235:genetics; D005798:Genes, Bacterial; D008826:Microbial Sensitivity Tests; D010957:Plasmids; D001618:beta-Lactamases / Q000096:biosynthesis* / Q000235:genetics; D047090:beta-Lactams
D000900:Anti-Bacterial Agents; D047090:beta-Lactams; D001618:beta-Lactamases