Gonadotropin regulation of rat ovarian lysosomes: existence of a hormone specific dual control mechanism.
Gonadotropic hormones PMSG (15 IU/rat), FSH (3 micrograms/rat), LH (9 micrograms/rat) and hCG (3 micrograms/rat) were shown to decrease the free cytosolic lysosomal enzymes during the acute phase of hormone action in rat ovaries. When isolated cells from such rats were analyzed for the cathepsin-D activity, the granulosa cells of the ovary showed a reduction in the free as well as in the total lysosomal enzyme activities in response to FSH/PMSG; the stromal and thecal compartment of the ovary showed a reduction only in the free activity in response to hCG/PMSG. The results suggest the presence of two distinct, target cell specific, mechanisms by which the lysosomal activity of the ovary is regulated by gonadotropins.
Dhanasekaran
N
N
Department of Biochemistry, Indian Institute of Science, Bangalore, India.
Moudgal
N R
NR
D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D002402:Cathepsin D / Q000378:metabolism; D006063:Chorionic Gonadotropin / Q000378:metabolism; D005260:Female; D005640:Follicle Stimulating Hormone / Q000378:metabolism; D006062:Gonadotropins / Q000378:metabolism / Q000494:pharmacology*; D006064:Gonadotropins, Equine / Q000378:metabolism; D007986:Luteinizing Hormone / Q000378:metabolism; D008247:Lysosomes / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism / Q000648:ultrastructure; D010053:Ovary / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism / Q000648:ultrastructure; D051381:Rats; D011919:Rats, Inbred Strains
D006063:Chorionic Gonadotropin; D006062:Gonadotropins; D006064:Gonadotropins, Equine; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D000135:Acid Phosphatase; D002402:Cathepsin D