Heparin interacts with a selenoprotein in human plasma.
The major selenium-containing peak at gel filtration of human serum on Sephadex G-150 had a Kav of 0.19, but at chromatography of heparinized plasma this peak had a Kav of 0.01, indicating an interaction between heparin and a major selenium-containing protein. The addition of protamine abolished the effect of heparin. The heparin-interacting protein was not identical to glutathione peroxidase. The data explain some previous discrepancies on selenium distribution in plasma due to the use of different anticoagulants.
Akesson
B
B
Department of Clinical Chemistry, University Hospital, University of Lund, Sweden.
Mårtensson
B
B
D001798:Blood Proteins; D002850:Chromatography, Gel; D005979:Glutathione Peroxidase / Q000097:blood; D006493:Heparin / Q000097:blood*; D006801:Humans; D011506:Proteins; D012643:Selenium / Q000097:blood; D051140:Selenoproteins
D001798:Blood Proteins; D011506:Proteins; D051140:Selenoproteins; D006493:Heparin; D005979:Glutathione Peroxidase; D012643:Selenium