The human Lyt-3 molecule requires CD8 for cell surface expression.
We have previously identified a monoclonal antibody, T8/2T8-5H7, which clustered serologically with CD8 monoclonal antibodies, but lacked reactivity with L cell transfectants expressing the human CD8 molecule (Lyt-2 homologue). Based on these observations, we postulated that T8/2T8-5H7 might recognize the human Lyt-3 gene product. To test this hypothesis, we have isolated a full-length cDNA encoding the human Lyt-3 molecule and have characterized its product in additional transfection experiments. The results of these studies indicate that the human Lyt-3 cDNA encodes a product recognized by the antibody T8/2T8-5H7. Interestingly, the human Lyt-3 molecule cannot be expressed alone, but requires the human Lyt-2 homologue for efficient cell surface expression. A heterodimer composed of the human Lyt-2 and Lyt-3 molecules may have importance in T cell-target cell interactions.
DiSanto
J P
JP
Effector Lymphocyte Biology, Memorial Sloan-Kettering Cancer Center, New York, NY 10021.
Knowles
R W
RW
Flomenberg
N
N
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000945:Antigens, Differentiation, T-Lymphocyte / Q000235:genetics* / Q000276:immunology; D000950:Antigens, Ly / Q000235:genetics* / Q000276:immunology; D000954:Antigens, Surface / Q000235:genetics; D001483:Base Sequence; D016827:CD8 Antigens; D002460:Cell Line; D002462:Cell Membrane / Q000276:immunology; D004247:DNA / Q000235:genetics*; D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation; D006801:Humans; D007739:L Cells; D051379:Mice; D008969:Molecular Sequence Data
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D000950:Antigens, Ly; D000954:Antigens, Surface; D016827:CD8 Antigens; D004247:DNA