title: Fusion transfer of dopamine DA1 receptors to Friend erythroleukemia cells.
abstract: Dopamine DA1 receptors were transferred from rat striatal membranes to Friend erythroleukemia cells (Fc) by membrane fusion. The Fc cells lack DA1 receptors but have a functional adenylate cyclase system. The striatal membranes bearing DA1 receptors were treated with N-ethylmaleimide (NEM) prior to fusion to inactivate their intrinsic adenylate cyclase activity. Fusion of the NEM-treated membranes and the Fc cells was induced by polyethylene glycol treatment to form a functional system de novo, which could be demonstrated by measuring the increase in cAMP production after addition of dopamine. This method provides a possibility to study the functional competence of receptors in neural tissue in more detail.
authors.forename: M
authors.initials: M
authors.lastname: Olasmaa
authors.forename: L
authors.initials: L
authors.lastname: Terenius
mesh_terms: D000818:Animals; D002421:Caudate Nucleus; D003342:Corpus Striatum; D005622:Friend murine leukemia virus; D006801:Humans; D004915:Leukemia, Erythroblastic, Acute; D007942:Leukemia, Experimental; D008297:Male; D008561:Membrane Fusion; D051381:Rats; D011919:Rats, Inbred Strains; D011954:Receptors, Dopamine; D017447:Receptors, Dopamine D1
chemical_list: D011954:Receptors, Dopamine; D017447:Receptors, Dopamine D1