Strong transcriptional promoter in the 5' upstream region of the human beta-actin gene.
We isolated the 4.3-kb nucleotide sequence 5' upstream from the coding region of the human beta-actin gene and tested its promoter function by using the chloramphenicol acetyl transferase assay. We found that the sequence required for efficient transcription was contained within the 1.2-kb DNA fragment of the actin 5'-noncoding region. The 1.2-kb fragment contained a TATA box, a CCAAT box and two highly conserved elements. The transcriptional start point was located 24 bp downstream from the TATA box. The 1.2-kb fragment showed strong promoter activity when it was transfected into human and rodent non-muscle cells. In human FL cells and in mouse NIH3T3 and L cells, the promoter activity of the 1.2-kb DNA fragment was greater than that of the SV40 early region.
Sugiyama
H
H
Department of Oncogene Research, Osaka University, Japan.
Niwa
H
H
Makino
K
K
Kakunaga
T
T
D000123:Acetyltransferases / Q000235:genetics; D000199:Actins / Q000235:genetics*; D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D015500:Chloramphenicol O-Acetyltransferase; D005796:Genes; D006801:Humans; D007700:Kinetics; D051379:Mice; D010957:Plasmids; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic; D014162:Transfection
D000199:Actins; D000123:Acetyltransferases; D015500:Chloramphenicol O-Acetyltransferase