Latex immunoassay of human serum Lp(a+) lipoprotein.
A sensitive latex immunoassay for human serum lipoprotein Lp(a+) is based on direct agglutination by Lp(a+) of latex particles coated with specific antibody. The agglutination is quantified by turbidimetry using a photometer at 360 nm. The stabilization of antibody-coated latex particles by bovine serum albumin occurs under well-defined conditions (pH, concentration of bovine serum albumin, and antibody loading of latex particles). The standard curve of serum lipoprotein Lp(a+) ranges from 0.05 to 1.15 mg/l. Inter- and intra-assay coefficients of variation were less than 8% and 3%, respectively. Results were well correlated with those obtained by electroimmunodiffusion (r = 0.98, n = 108).
Vu-Dac
N
N
Chekkor
A
A
Parra
H
H
Duthilleul
P
P
Fruchart
J C
JC
D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007840:Latex; D017270:Lipoprotein(a); D008074:Lipoproteins / Q000097:blood*
D007840:Latex; D017270:Lipoprotein(a); D008074:Lipoproteins