title: Characterization of crystals of xylose isomerase from Streptomyces violaceoniger.
abstract: Crystals of the tetrameric xylose isomerase from Streptomyces violaceoniger have been examined by x-ray analysis. Octahedral crystals with a maximum dimension of 0.7 mm were grown from ammonium sulfate solution. They possess the symmetry of P4(1)2(1)2 or P4(3)2(1)2 space groups, which are crystallographically indistinguishable. The unit cell dimensions are a = b = 140 A and c = 134 A. There is one tetramer of molecular weight 160,000 per asymmetric unit. The crystals diffract to 2.2 A.
authors.affiliation: Department of Chemistry, Massachusetts Institute of Technology, Cambridge 02139.
authors.forename: A
authors.initials: A
authors.lastname: Glasfeld
authors.forename: G K
authors.initials: GK
authors.lastname: Farber
authors.forename: D
authors.initials: D
authors.lastname: Ringe
authors.forename: T
authors.initials: T
authors.lastname: Marcel
authors.forename: D
authors.initials: D
authors.lastname: Drocourt
authors.forename: G
authors.initials: G
authors.lastname: Tiraby
authors.forename: G A
authors.initials: GA
authors.lastname: Petsko
mesh_terms: D019747:Aldose-Ketose Isomerases; D002238:Carbohydrate Epimerases / Q000302:isolation & purification; D046911:Macromolecular Substances; D011487:Protein Conformation; D013302:Streptomyces / Q000201:enzymology*; D014961:X-Ray Diffraction / Q000379:methods
chemical_list: D046911:Macromolecular Substances; D002238:Carbohydrate Epimerases; D019747:Aldose-Ketose Isomerases; C023298:xylose isomerase