title: Phosphorylation of the Ca2+-pumping ATPase of heart sarcolemma and erythrocyte plasma membrane by the cAMP-dependent protein kinase.
abstract: The Ca2+ ATPase of heart sarcolemma was stimulated by the exposure of sarcolemma vesicles to ATP and the catalytic subunit of the cAMP-dependent protein kinase. The effect of the phosphorylation system was primarily on the Km(Ca2+) of the pumping ATPase. The ATPase purified from heart sarcolemma or erythrocytes became phosphorylated under the conditions mentioned above. Hydroxylamine treatment of the labeled ATPase has shown that the phosphorylation was additive to be acylphosphate formed on the ATPase during the reaction cycle. The stoichiometry of the kinase-promoted phosphorylation (i.e. the fraction of the ATPase molecules that became labeled) approached 30% with both the heart and the erythrocyte enzyme.
authors.forename: L
authors.initials: L
authors.lastname: Neyses
authors.forename: L
authors.initials: L
authors.lastname: Reinlib
authors.forename: E
authors.initials: E
authors.lastname: Carafoli
mesh_terms: D000818:Animals; D000936:Antigen-Antibody Complex; D002118:Calcium / Q000378:metabolism; D000252:Calcium-Transporting ATPases / Q000097:blood / Q000302:isolation & purification / Q000378:metabolism*; D002417:Cattle; D004910:Erythrocyte Membrane / Q000201:enzymology*; D007106:Immune Sera; D007700:Kinetics; D008970:Molecular Weight; D009206:Myocardium / Q000201:enzymology*; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism*; D012508:Sarcolemma / Q000201:enzymology*
chemical_list: D000936:Antigen-Antibody Complex; D007106:Immune Sera; D011494:Protein Kinases; D000252:Calcium-Transporting ATPases; D002118:Calcium