title: Endothelin stimulates phospholipase C in cultured vascular smooth muscle cells.
abstract: Cultured vascular smooth muscle cells from bovine and rat thoracic aortae and from human omental vessels have been examined for cellular responses to endothelin. In myo-[3H]-inositol-prelabelled cells endothelin induced a rapid (within 30 sec) and protracted increase of [3H]-inositol content in inositol bis- and tris-phosphates. Concomitantly, significant polyphosphoinositide hydrolysis occurred within 30 sec. Accumulation of [3H]-inositol monophosphate and hydrolysis of phosphatidylinositol were delayed. In cells prelabelled with [3H]-arachidonic acid endothelin promoted rapid production of [3H]-diacylglycerol which decayed slowly toward control values after reaching maximum levels (1-2 min). Half-maximally effective concentrations of endothelin for all these cellular responses were comparable (approximately 3-7 nM) and not significantly different between the vascular cell isolates. The involvement of the phospholipase C-signal transduction pathway in mediating endothelin-induced vasoconstriction is invoked.
authors.affiliation: Department of Research, University Hospital, Basel, Switzerland.
authors.forename: T J
authors.initials: TJ
authors.lastname: Resink
authors.forename: T
authors.initials: T
authors.lastname: Scott-Burden
authors.forename: F R
authors.initials: FR
authors.lastname: Bühler
mesh_terms: D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism; D002417:Cattle; D002478:Cells, Cultured; D004075:Diglycerides / Q000378:metabolism; D016232:Endothelins; D004730:Endothelium, Vascular; D006801:Humans; D006868:Hydrolysis; D007294:Inositol / Q000378:metabolism; D007295:Inositol Phosphates / Q000378:metabolism; D007700:Kinetics; D009131:Muscle, Smooth, Vascular / Q000201:enzymology*; D010455:Peptides / Q000494:pharmacology*; D010716:Phosphatidylinositols / Q000378:metabolism; D051381:Rats; D010738:Type C Phospholipases / Q000378:metabolism*
chemical_list: D001095:Arachidonic Acids; D004075:Diglycerides; D016232:Endothelins; D007295:Inositol Phosphates; D010455:Peptides; D010716:Phosphatidylinositols; D016718:Arachidonic Acid; D007294:Inositol; D010738:Type C Phospholipases