Crossmatching on platelets by flow cytometry.
1. HLA antigens on platelets can be detected by alloantisera used for typing lymphocytes. 2. There is a general correlation between reactivity of sera against platelets and reactivity against T cells. However, some SLE patients have broadly reactive antibodies to T cells that are much less reactive to platelets. These sera may contain IgG autoantibody to non-HLA T-cell autoantigens. 3. The Pl-FCXM may be both more sensitive than the conventional cytotoxic crossmatching techniques and more discriminating than the T-FCXM in detecting relevant deleterious antibodies. All 11 patients with a negative platelet FXCM had functioning kidneys at 1 month, whereas 7 of 12 patients with a positive Pl-FCXM had nonfunctional kidneys at 1 month (p = 0.003). 4. It is suggested that the Pl-FCXM offers a simple, sensitive, and effective method of crossmatching without the high false positive rate of T-FCXM.
Wang
G X
GX
Terashita
G Y
GY
Terasaki
P I
PI
D001792:Blood Platelets / Q000276:immunology*; D005069:Evaluation Studies as Topic; D005189:False Positive Reactions; D005434:Flow Cytometry; D006084:Graft Rejection; D006680:HLA Antigens / Q000032:analysis*; D015789:HLA-A2 Antigen / Q000276:immunology; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans; D007518:Isoantibodies / Q000032:analysis; D007677:Kidney Function Tests; D016030:Kidney Transplantation / Q000276:immunology*; D010043:Outcome and Process Assessment, Health Care; D013601:T-Lymphocytes / Q000276:immunology
D006680:HLA Antigens; D015789:HLA-A2 Antigen; D007518:Isoantibodies