Isolation and characterization of 5-lipoxygenase from tulip bulbs.
An unique membrane bound lipoxygenase was isolated and purified from purple star tulip bulbs with a specific activity of 5.2 mu moles O2 consumed.min-1.mg-1 protein. The purified tulip enzyme exhibits regiospecificity for O2 insertion at C-5 of the arachidonic acid molecule. Identification of the reaction product was confirmed as 5-hydroperoxyeicosatetraenoic acid by analytical criteria which included: cochromatography with the authentic compound, as well as mass spectral and 1H-NMR analysis. Thus, the enzyme from tulip bulbs appears to be different from the cytosolic lipoxygenase from potato tubers, which exhibits non-regiospecificity in terms of O2 incorporation. However, the purified tulip lipoxygenase showed a strong immunological crossreactivity with antiserum raised against the purified potato lipoxygenase, indicating close immunological relationship with the other plant lipoxygenases.
Reddanna
P
P
Department of Veterinary Science, Pennsylvania State University, University Park 16802.
Whelan
J
J
Reddy
P S
PS
Reddy
C C
CC
D001094:Arachidonate 5-Lipoxygenase / Q000302:isolation & purification* / Q000378:metabolism; D001091:Arachidonate Lipoxygenases / Q000302:isolation & purification*; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism; D015153:Blotting, Western; D002851:Chromatography, High Pressure Liquid; D003600:Cytosol / Q000201:enzymology; D007526:Isoelectric Point; D007700:Kinetics; D015289:Leukotrienes / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008970:Molecular Weight; D009685:Nuclear Envelope / Q000201:enzymology; D010100:Oxygen / Q000378:metabolism; D010944:Plants / Q000201:enzymology* / Q000648:ultrastructure; D013045:Species Specificity
D001095:Arachidonic Acids; D015289:Leukotrienes; D016718:Arachidonic Acid; C025348:arachidonic acid 5-hydroperoxide; D001091:Arachidonate Lipoxygenases; D001094:Arachidonate 5-Lipoxygenase; D010100:Oxygen