Novel structure of the cellular mannan of the pathogenic yeast Candida krusei.
On the basis of methylation analysis, 13C- and 1H-n.m.r. spectroscopic data, and the results of immunological methods, a new structure for the cell-wall alpha-D-mannan of the pathogenic yeast Candida krusei is proposed. In contrast to the alpha-D-mannans of other pathogenic yeasts of the Candida species, which have (1----6)-linked main chains and many (1----2)- and/or (1----3)-linked side chains, the C. krusei mannan is lightly branched and contains (1----2) and (1----6) linkages in the ratio 3:1.
Kogan
G
G
Institute of Chemistry, Slovak Academy of Sciences, Bratislava.
Pavliak
V
V
Sandula
J
J
Masler
L
L
D000107:Acetylation; D002175:Candida / Q000032:analysis*; D002236:Carbohydrate Conformation; D002473:Cell Wall / Q000032:analysis; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D005779:Immunodiffusion; D007202:Indicators and Reagents; D009682:Magnetic Resonance Spectroscopy; D008351:Mannans / Q000276:immunology / Q000302:isolation & purification*; D008745:Methylation
D007202:Indicators and Reagents; D008351:Mannans