Visualization of exocellular lectins in the entomopathogenic fungus Conidiobolus obscurus.
We demonstrated the presence of lectins binding to glucose and N-acetylglucosamine on the surface of Conidiobolus obscurus spores by using glycosylated serum albumins substituted with fluorescent dyes and colloidal gold. The role of these exocellular lectins was examined in relation to the adhesion of the fungal spores to their host insect and the pathogenicity of the fungus.
Latgé
J P
JP
Unité de Mycologie, Institut Pasteur, Paris, France.
Monsigny
M
M
Prévost
M C
MC
D000117:Acetylglucosamine / Q000378:metabolism; D000818:Animals; D001042:Aphids / Q000382:microbiology; D003102:Colloids; D004773:Entomophthora / Q000032:analysis*; D005456:Fluorescent Dyes; D005658:Fungi / Q000032:analysis*; D005947:Glucose / Q000378:metabolism; D017127:Glycation End Products, Advanced; D006031:Glycosylation; D006046:Gold; D037102:Lectins / Q000032:analysis* / Q000378:metabolism; D008856:Microscopy, Fluorescence; D012709:Serum Albumin; D013172:Spores, Fungal / Q000032:analysis; D000093423:Glycated Serum Albumin
D003102:Colloids; D005456:Fluorescent Dyes; D017127:Glycation End Products, Advanced; D037102:Lectins; D012709:Serum Albumin; D006046:Gold; D005947:Glucose; D000117:Acetylglucosamine; D000093423:Glycated Serum Albumin