Evaluation of a test kit for determination of serum immunoglobulin G concentration in foals.
The accuracy of an immunoglobulin (Ig) G test kit for the semiquantitative measurement of IgG concentration was evaluated with serum from 88 foals. Failure of passive transfer (IgG less than 400 mg/dl) was correctly identified in each of 34 samples, and partial failure of passive transfer (400 less than or equal to IgG less than 800 mg/dl) was correctly identified in each of nine samples. Evidence of adequate passive transfer (IgG greater than or equal to 800 mg/dl) was detected in 44 of 45 samples. One sample with 800 mg/dl or more of IgG was incorrectly classified as a partial failure of passive transfer (Kendall Tau - b = .975). The high degree of accuracy, especially without any errors of overestimation of IgG concentrations, indicated that the IgG test kit should be a useful assay for rapidly determining the passive transfer status of foals.
Bertone
J J
JJ
Department of Veterinary Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins.
Jones
R L
RL
Curtis
C R
CR
D000818:Animals; D006736:Horses / Q000276:immunology*; D007112:Immunity, Maternally-Acquired; D007116:Immunization, Passive / Q000662:veterinary*; D005779:Immunodiffusion; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000032:analysis*; D011933:Reagent Kits, Diagnostic / Q000662:veterinary*
D007074:Immunoglobulin G; D011933:Reagent Kits, Diagnostic