title: Lipid interactions with in vitro development of mammalian zygotes.
abstract: Rabbit zygotes were tested for their ability to sequester radiolabeled acetate, oleate, and arachidonate in intracellular lipid. Radiolabeled arachidonic acid was concentrated 170 +/- 28-fold (mean +/- SEM) and oleic acid was concentrated 105 +/- 26-fold in zygotic lipids during 6 hr of culture when compared with the initial concentrations in culture medium. Acetate was not concentrated into lipids by cultured zygotes. Both long chain fatty acids were incorporated mainly as triglyceride. Polydimethylsiloxane fluid, used to cover the microdroplets of medium during culture, demonstrated lipophilic properties. This characteristic was utilized to indirectly transfer lipids to culture medium, permitting examination only of lipoidal properties of test extracts on embryonal development. For rabbit zygotes, blood plasma extract was detrimental and whole blood extract was beneficial for embryonal cleavage rates during the first 24 hr of culture. A higher proportion of mouse zygotes developed to blastocysts when cultured in modified Ham's F-10 medium compared to BMOC medium, and this difference was negated by inclusion of a lipid extract prepared from rabbit oviductal fluid in the culture system. Comparison of fatty acid analyses of the lipid extracts with development rates of zygotes suggests that modified rates of embryo development may be associated with ratios of individual fatty acids presented to the culture medium rather than with the presence of any single fatty acid.
authors.affiliation: Reproduction Laboratory, Beltsville Agricultural Research Center, Maryland 20705.
authors.forename: R A
authors.initials: RA
authors.lastname: Waterman
authors.forename: R J
authors.initials: RJ
authors.lastname: Wall
mesh_terms: D000085:Acetates / Q000493:pharmacokinetics; D019342:Acetic Acid; D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000493:pharmacokinetics; D003470:Culture Media / Q000493:pharmacokinetics; D004058:Diffusion; D005227:Fatty Acids / Q000493:pharmacokinetics*; D066298:In Vitro Techniques; D051379:Mice; D019301:Oleic Acid; D009829:Oleic Acids / Q000493:pharmacokinetics; D011817:Rabbits; D014020:Tissue Extracts; D015053:Zygote / Q000378:metabolism*
chemical_list: D000085:Acetates; D001095:Arachidonic Acids; D003470:Culture Media; D005227:Fatty Acids; D009829:Oleic Acids; D014020:Tissue Extracts; D016718:Arachidonic Acid; D019301:Oleic Acid; D019342:Acetic Acid