Prostanoids modulate reflex micturition by acting through capsaicin-sensitive afferents.
Topical application of exogenous prostanoids (PGE2, TBX B2) on the serosal surface of the urinary bladder of urethane-anaesthetized rats activated reflex micturition. Likewise, intravesical instillation of PGE2 during the cystometrogram lowered the threshold for reflex micturition. Both effects were prevented by systemic capsaicin desensitization (50 mg/kg s.c., 4 days before). Indomethacin pretreatment and systemic capsaicin desensitization each increased the micturition threshold without affecting the amplitude of micturition contraction. However, the effect of the two treatments combined was not greater than the effect of either alone. These findings support the idea that endogenous prostanoids facilitate reflex micturition by stimulating or sensitizing, directly or indirectly, the subset of bladder mechanoreceptors which is capsaicin-sensitive in adult rats.
Maggi
C A
CA
Pharmacology Department, A. Menarini Pharmaceuticals, Florence, Italy.
Giuliani
S
S
Conte
B
B
Furio
M
M
Santicioli
P
P
Meli
P
P
Gragnani
L
L
Meli
A
A
D000818:Animals; D002211:Capsaicin / Q000494:pharmacology*; D015232:Dinoprostone; D007213:Indomethacin / Q000494:pharmacology; D008297:Male; D011458:Prostaglandins E / Q000008:administration & dosage / Q000494:pharmacology / Q000502:physiology*; D051381:Rats; D012018:Reflex / Q000187:drug effects*; D013929:Thromboxane B2 / Q000502:physiology*; D001743:Urinary Bladder / Q000187:drug effects / Q000294:innervation; D014554:Urination / Q000187:drug effects*
D011458:Prostaglandins E; D013929:Thromboxane B2; D015232:Dinoprostone; D002211:Capsaicin; D007213:Indomethacin