title: Elimination of [14C]heptachlor from body stores of lactating ewes treated with ovine growth hormone.
abstract: Elimination of [14C]heptachlor from body burdens of sheep was measured using mature ewes nursing single offspring, and the influence of exogenous ovine growth hormone (oGH) on elimination was studied. Six ewes (62 +/- 2.5 kg BW) were dosed (i.p.) once with [14C]heptachlor (2.04 mg/kg Bw; .88 microCi/mg heptachlor) and three were treated additionally with oGH (oGH; 5 mg/d) for 21 d. Three additional ewes served as controls. Excreta were collected each day for 21 d. Milk and blood were collected every 3rd d until ewes were euthanized at d 21. 14C activity was measured in excreta, milk, blood and tissues. Total cumulative activity of [14C]heptachlor and(or) metabolites in excreta (21 d) did not differ (P greater than .20) in ewes given oGH (25 +/- 2%) vs none (23 +/- 2%). Milk yield and protein content were unaffected (P greater than .10) by oGH. Ewes given oGH eliminated 2.2 +/- 2% of total 14C dosage into milk during 21 d, whereas ewes untreated with oGH eliminated 1.3 +/- .2% (P less than .10); total 14C activity eliminated into milk plus excreta was similar for ewes given oGH or none (P greater than .10). For all six ewes, half-times (T1/2) for distribution and elimination of 14C activity (heptachlor and metabolites) were 1.5 d and 11.7 d, respectively. Blood concentrations of 14C activity during 21 d yielded elimination half-time as 23 d. Unlike bovines, which eliminate heptachlor slowly (T1/2 approximately 70 to 80 d) and mainly into milk fat, lactating ovines eliminated heptachlor and(or) metabolites mainly into excreta and about sixfold faster than bovines.
authors.affiliation: Dept. of Anim. and Range Sci., New Mexico State University, Las Cruces 88003.
authors.forename: D W
authors.initials: DW
authors.lastname: Holcombe
authors.forename: G S
authors.initials: GS
authors.lastname: Smith
authors.forename: M F
authors.initials: MF
authors.lastname: Khan
authors.forename: D M
authors.initials: DM
authors.lastname: Hallford
authors.forename: K
authors.initials: K
authors.lastname: Rozman
mesh_terms: D000818:Animals; D001822:Body Burden; D005260:Female; D013006:Growth Hormone / Q000008:administration & dosage / Q000494:pharmacology*; D006533:Heptachlor / Q000493:pharmacokinetics*; D007774:Lactation / Q000378:metabolism*; D011247:Pregnancy; D012756:Sheep / Q000378:metabolism*
chemical_list: D006533:Heptachlor; D013006:Growth Hormone