title: Effects of 8-aminoguanosine on the toxicity of guanosine and deoxyguanosine for malignant and normal lymphoid cells.
abstract: The toxicity of guanosine and deoxyguanosine in the presence or absence of the purine nucleoside phosphorylase inhibitor, 8-aminoguanosine, for malignant lymphoid cell lines and mitogen-stimulated peripheral blood lymphocytes has been studied. Deoxyguanosine inhibited the proliferation of lymphoid cells more strongly than guanosine. Addition of 100 microM 8-aminoguanosine neither enhanced nor diminished the toxicity of guanosine to the lymphoid cells. Only the toxicity of deoxyguanosine for the leukemic T cell line, MOLT 4, and the leukemic nonBnonT cell line, KM-3, was enhanced by the addition of 100 microM 8-aminoguanosine. These data suggest a possible role of purine nucleoside phosphorylase inhibitors in the treatment of lymphoproliferative disorders of the T-acute lymphoblastic leukemia (ALL) as well as the nonBnonT-ALL subclass.
authors.affiliation: Centre for Pediatric Oncology SE Netherlands, Department of Pediatrics, University Hospital, St. Radboud, Nijmegen.
authors.forename: P M
authors.initials: PM
authors.lastname: van der Kraan
authors.forename: P M
authors.initials: PM
authors.lastname: van Zandvoort
authors.forename: R A
authors.initials: RA
authors.lastname: De Abreu
authors.forename: J A
authors.initials: JA
authors.lastname: Bakkeren
mesh_terms: D002478:Cells, Cultured; D003848:Deoxyguanine Nucleotides / Q000494:pharmacology; D003849:Deoxyguanosine / Q000633:toxicity*; D006151:Guanosine / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000633:toxicity*; D006801:Humans; D007938:Leukemia / Q000188:drug therapy; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects*
chemical_list: D003848:Deoxyguanine Nucleotides; D006151:Guanosine; C032533:8-aminoguanosine; C029603:deoxyguanosine triphosphate; D003849:Deoxyguanosine