title: Salt soluble cross-linked elastin: formation and composition of fibers.
abstract: Cross-linked elastin has been isolated from the salt extract of sheep vascular tissue by means of hydrophobic interaction chromatography on a column of decyl-agarose. Dialysis of the dimethylformamide and sodium dodecyl sulphate column eluates against distilled water produced a precipitate that was fibrous and that resembled insoluble elastin fibers. As judged by amino acid analyses and SDS-polyacrylamide gel electrophoresis, this precipitation resulted in further purification of the soluble cross-linked elastin. Similar chromatography and precipitation of oxalic acid solubilized cross-linked elastin (alpha-elastin) produced identical results.
authors.forename: J N
authors.initials: JN
authors.lastname: Manning
authors.forename: P F
authors.initials: PF
authors.lastname: Davis
authors.forename: N S
authors.initials: NS
authors.lastname: Greenhill
authors.forename: A J
authors.initials: AJ
authors.lastname: Sigley
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001808:Blood Vessels / Q000032:analysis; D002847:Chromatography, Agarose; D003956:Dialysis; D004549:Elastin / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D008855:Microscopy, Electron, Scanning; D011487:Protein Conformation; D012756:Sheep; D012965:Sodium Chloride; D012995:Solubility
chemical_list: D000596:Amino Acids; D012965:Sodium Chloride; D004549:Elastin