Growth-coupled changes in glucosaminoglycans (heparan sulfate and hyaluronic acid) in normal and transformed human fibroblasts.
Changes in glycosaminoglycans (GAGs) were investigated in relation to cell density, growth and transformation of human fibroblasts. Relative amounts (percentages of the total GAGs) of heparan sulfate (HS) increased and those of hyaluronic acid (HA) decreased in growth-reduced (serum-starved, exogenous HS-treated and dense) cultures of normal (WI-38) cells. In contrast, transformed (WI-38 CT-1) cells exerted such GAG changes only in serum-starved cultures, but not in HS-treated or dense cultures. These results indicate that the changes in glucosaminoglycans (G1cAGs) (HS and HA) is coupled exclusively with cell growth.
Matuoka
K
K
Mitsui
Y
Y
Murota
S
S
D000818:Animals; D002452:Cell Count; D002455:Cell Division / Q000187:drug effects; D002472:Cell Transformation, Viral; D003470:Culture Media; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology*; D005944:Glucosamine / Q000378:metabolism; D006025:Glycosaminoglycans / Q000378:metabolism*; D006367:HeLa Cells / Q000378:metabolism; D006497:Heparan Sulfate / Q000378:metabolism* / Q000494:pharmacology; D006801:Humans; D006820:Hyaluronic Acid / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D011817:Rabbits; D012710:Serum Albumin, Bovine / Q000494:pharmacology
D003470:Culture Media; D005944:Glucosamine; D006025:Glycosaminoglycans; D006497:Heparan Sulfate; D006820:Hyaluronic Acid; D012710:Serum Albumin, Bovine