[The role of the cytoskeleton in lymphocyte stimulation].
The influence of the cytoskeleton active drugs Cytochalasin B and Colchicine on the 3H-thymidine and 14C-uridine incorporation of Con A stimulated lymphocytes of mice was studied. Both drugs had no influence on the entry of the Con A incubated lymphocytes into the G1-phase but inhibited significantly the entry into the S-phase of the cell cycle. Cytochalasin B and Colchicine differ in their effect on the release of charge changing lymphokines. Colchicine did not influence this release in contrast to Cytochalasin B. The results show that both Cytochalasin B and Colchicine suppress cellular processes which are necessary for going beyond the commitment point. A possible importance of the microfilaments for the release of lymphokines and of the microtubuli for the expression of lymphokine receptors is discussed.
Hückel
C
C
Werner
H
H
Brock
J
J
D000818:Animals; D002250:Carbon Radioisotopes; D002453:Cell Cycle / Q000187:drug effects; D003078:Colchicine / Q000494:pharmacology*; D003208:Concanavalin A / Q000494:pharmacology; D003571:Cytochalasin B / Q000494:pharmacology*; D003599:Cytoskeleton / Q000187:drug effects*; D005260:Female; D008213:Lymphocyte Activation / Q000187:drug effects*; D051379:Mice; D008808:Mice, Inbred CBA; D008937:Mitomycins / Q000494:pharmacology; D011691:Puromycin / Q000494:pharmacology; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors; D014316:Tritium; D014529:Uridine / Q000378:metabolism
D002250:Carbon Radioisotopes; D008937:Mitomycins; D014316:Tritium; D003208:Concanavalin A; D003571:Cytochalasin B; D011691:Puromycin; D003078:Colchicine; D013936:Thymidine; D014529:Uridine
Die Rolle des Zytoskeletts bei der Lymphozytenstimulierung.