Extensive deletions and insertions in different MHC supratypes detected by pusled field gel electrophoresis.
The genomic organization of the human MHC was examined in multiple examples of six different supratypes using pulsed field electrophoresis (PFGE) after digestion of genomic DNA with infrequency cutting restriction endonucleases. Differences in restriction fragment length and band intensity were shown to be specific for each supratype. Mapping of the MHC revealed that each supratype contains previously undescribed deletions and insertions between HLA B and DQ regions.
Tokunaga
K
K
Department of Clinical Immunology, Royal Perth Hospital, Perth, Western Australia.
Saueracker
G
G
Kay
P H
PH
Christiansen
F T
FT
Anand
R
R
Dawkins
R L
RL
D002872:Chromosome Deletion; D002874:Chromosome Mapping; D002896:Chromosomes, Human, Pair 6; D004586:Electrophoresis / Q000379:methods*; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D006801:Humans; D008285:Major Histocompatibility Complex; D005810:Multigene Family; D015183:Restriction Mapping