Induction of metaphase chromosome condensation in human sperm by Xenopus egg extracts.
Cell-free extracts of Xenopus eggs cause permeabilized Xenopus sperm to form pronuclei, which condense into metaphase chromosomes when the cytosol from metaphase-arrested unfertilized eggs is added to the extracts. In this paper, the ability of these cell-free extracts to cause similar changes in permeabilized human sperm was examined. Sperm that had been treated with the disulfide reducing agent dithiothreitol formed pronuclei, whereas untreated sperm did not. The addition of metaphase cytosol to the extracts caused the pronuclei to form metaphase chromosomes but only after incubation times that were two to three times longer than those required for Xenopus sperm nuclei. These results indicate that despite species differences, the Xenopus egg extracts can be used to visualize the chromosomes of human sperm and possibly those of other species.
Lohka
M J
MJ
Department of Pharmacology, University of Colorado School of Medicine, Denver 80262.
Maller
J L
JL
D000818:Animals; D002457:Cell Extracts / Q000494:pharmacology*; D002877:Chromosomes, Human; D005260:Female; D006801:Humans; D008297:Male; D008677:Metaphase / Q000187:drug effects*; D009865:Oocytes / Q000032:analysis*; D013084:Sperm-Ovum Interactions; D013094:Spermatozoa / Q000166:cytology*; D014020:Tissue Extracts / Q000494:pharmacology*; D014982:Xenopus laevis
D002457:Cell Extracts; D014020:Tissue Extracts