Lysosomal isoenzyme profiles used to classify a case of acute undifferentiated leukaemia.
Lysosomal enzyme activities and isoenzyme profiles were measured in lymphoid and non-lymphoid leukaemic cells from childhood patients. High activities, especially of beta-hexosaminidase and alpha-mannosidase, were associated with leukaemic cells of myeloid or monocytic origin. Leukaemic cells from two children with acute myeloid leukaemia had a relative reduction in the B isoenzyme of beta-hexosaminidase activity, whereas in patients with non T, non B cell acute lymphoblastic leukaemia, intermediate beta-hexosaminidase isoenzymes were expressed. A patient is described on whom conventional marker studies were either negative or equivocal, but lysosomal enzyme markers were consistent with a myeloid leukaemia. This observation was supported by the clinical course of this patient.
Eden
O B
OB
Darbyshire
P
P
Simpson
R M
RM
Besley
G T
GT
Moss
S
S
Gentle
T
T
D002648:Child; D002675:Child, Preschool; D005260:Female; D006596:Hexosaminidases / Q000032:analysis; D006801:Humans; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000032:analysis*; D015470:Leukemia, Myeloid, Acute / Q000175:diagnosis* / Q000201:enzymology; D008247:Lysosomes / Q000201:enzymology*; D008361:Mannosidases / Q000032:analysis; D043323:alpha-Mannosidase; D001619:beta-N-Acetylhexosaminidases
D007527:Isoenzymes; D006596:Hexosaminidases; D008361:Mannosidases; D043323:alpha-Mannosidase; D001619:beta-N-Acetylhexosaminidases