title: Validation of a single-step procedure for the objective assessment of sperm motility characteristics.
abstract: A relatively cheap method is described for the objective assessment of sperm concentration and motility characteristics. The method uses a digitizing tablet with cursor, a micro-computer and a phase-contrast or dark-field microscope equipped with a drawing tube. With this technique the following are accurately assessed: sperm concentration, percentage motility, motility grading, concentration of grade a motile spermatozoa, sperm velocity, linear velocity, linearity and angularity. The data are acquired in less than 5 min. Validation studies reveal this method to be accurate, reproducible (coefficient of variation of motility characteristics = less than 7%) and clinically useful.
authors.affiliation: Department of Internal Medicine, State University Hospital, Ghent, Belgium.
authors.forename: A
authors.initials: A
authors.lastname: Hinting
authors.forename: F
authors.initials: F
authors.lastname: Schoonjans
authors.forename: F
authors.initials: F
authors.lastname: Comhaire
mesh_terms: D000465:Algorithms; D006801:Humans; D008297:Male; D012661:Semen / Q000032:analysis; D012984:Software; D013081:Sperm Motility; D013094:Spermatozoa / Q000032:analysis