Kinetic characterization of dopamine as a suicide substrate of tyrosinase.
A kinetic study of the inactivation of frog epidermis tyrosinase by a suicide substrate dopamine hydrochloride is described. The kinetic parameters and constants which characterize this reaction have been determined and the effects of pH and the stoichiometric inhibition by chloride have been considered.
Tudela
J
J
Departmento de Bioquimica, Facultad de Medicina, Universidad de Murcia, Spain.
Garcia-Canovas
F
F
Varón
R
R
Jimenez
M
M
Garcia-Carmona
F
F
Lozano
J A
JA
D000818:Animals; D004156:Catechol Oxidase / Q000037:antagonists & inhibitors*; D004298:Dopamine / Q000378:metabolism / Q000494:pharmacology*; D004817:Epidermis / Q000201:enzymology; D007700:Kinetics; D008433:Mathematics; D008962:Models, Theoretical; D014442:Monophenol Monooxygenase / Q000037:antagonists & inhibitors*; D011898:Ranidae
D004156:Catechol Oxidase; D014442:Monophenol Monooxygenase; D004298:Dopamine