Different distribution of DNA polymerases alpha and beta in bone marrow and peripheral blood from human leukemia.
An activity gel method was used to analyze the catalytic polypeptides of polymerases alpha and beta in human acute myeloblastic and lymphoblastic leukemia. A 175 kDa alpha-polymerase was found in 85% of bone marrow and in 57% of peripheral blood samples. At variance, a 40 kDa beta-polymerase was found in 94% of peripheral blood and only in 12% of bone marrow samples. No difference in the pattern of polymerase expression was found according to the type of leukemia and the disease status. The role of these enzymes in leukemia and their implications in drug sensitivity are discussed.
Lori
F
F
Istituto di Patologia Medica, Università di Parma, Italy.
Scovassi
A I
AI
Brusamolino
E
E
Casoli
C
C
Starcich
R
R
D000293:Adolescent; D000328:Adult; D000368:Aged; D001853:Bone Marrow / Q000201:enzymology*; D002648:Child; D004256:DNA Polymerase I / Q000097:blood / Q000378:metabolism*; D004257:DNA Polymerase II / Q000097:blood / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D007945:Leukemia, Lymphoid / Q000097:blood / Q000201:enzymology*; D015470:Leukemia, Myeloid, Acute / Q000097:blood / Q000201:enzymology*; D008297:Male; D008875:Middle Aged; D008970:Molecular Weight
D004256:DNA Polymerase I; D004257:DNA Polymerase II