Double immunohistochemical demonstration of antigen expression and DNA-incorporated 5-bromodeoxyuridine in frozen and plastic embedded sections.
In this study a double immunohistochemical staining procedure is described for the simultaneous visualization of antigen expressing cells and replicating cells. Cell surface antigen expression was marked with a monoclonal antibody against I a (His 19) or a monoclonal antibody against a membrane component of the cells of the monocyte-macrophage lineage (ED2). Replicating cells were detected by the incorporation of 5-bromodeoxyuridine. The method was applied sequentially. On frozen sections two peroxidase labeled reagents were used with two different substrates yielding a red and a dark-blue black reaction product. On plastic-embedded sections a peroxidase and an alkaline phosphatase labeled reagent were applied resulting in a brown and a blue reaction product.
Harms
G
G
Department of Pathology, State University of Groningen, The Netherlands.
van Goor
H
H
Koudstaal
J
J
de Ley
L
L
Hardonk
M J
MJ
D000818:Animals; D001973:Bromodeoxyuridine / Q000378:metabolism*; D004261:DNA Replication; D005615:Freezing; D007150:Immunohistochemistry / Q000379:methods*; D008297:Male; D008689:Methacrylates; D008867:Microtomy; D051381:Rats; D011919:Rats, Inbred Strains
D008689:Methacrylates; D001973:Bromodeoxyuridine