Dexamethasone modulated protein synthesis in polymorphonuclear leukocytes: response in rheumatoid arthritis.
Nine polypeptides whose rate of synthesis is modulated in polymorphonuclear leukocytes (PMNL) by dexamethasone were studied by 2-dimensional polyacrylamide gel electrophoresis. In each case, there was a time lag between addition of dexamethasone and the appearance of any change in rate of synthesis. In PMNL from a group of patients with rheumatoid arthritis, dexamethasone modulated the synthesis of the same polypeptides as in PMNL from a group of healthy volunteers, but the degree of response of their polypeptides to dexamethasone was significantly reduced as measured by assaying the level of synthesis of a polypeptide of molecular weight 16,000. Reduced responses of PMNL polypeptide synthesis to glucocorticoid may allow the development of rheumatoid inflammation despite apparently adequate levels of endogenous corticosteroids.
Blowers
L E
LE
Rheumatic Diseases Centre, University of Manchester, Hope Hospital, Salford, England.
Jayson
M I
MI
Jasani
M K
MK
D001172:Arthritis, Rheumatoid / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D002478:Cells, Cultured; D003907:Dexamethasone / Q000494:pharmacology*; D006801:Humans; D008715:Methionine / Q000378:metabolism; D008970:Molecular Weight; D009504:Neutrophils / Q000378:metabolism*; D010452:Peptide Biosynthesis; D011239:Prednisolone / Q000627:therapeutic use; D012016:Reference Values
D003907:Dexamethasone; D011239:Prednisolone; D008715:Methionine