Aminomethyl coumarin acetic acid and fluorescein isothiocyanate in detection of leishmanial antibodies: a comparative study.
Fluorescein isothiocyanate incorporated in the immunofluorescent antibody test system used for serodiagnosis of leishmanial and other infections suffers certain inherent disadvantages. Aminomethyl coumarin acetic acid, a new fluorescent label for protein, yields better results regarding non-specific background staining and stability, with slower photodecomposition under ultra-violet light when used for detection of leishmanial antibodies. The new labelling agent emits a yellow/green colour with an excitation filter of 450-490 nm and a barrier filter of 520 nm.
Eldaw
A
A
College of Medicine and Medical Sciences, Arabian Gulf University, State of Bahrain.
Khalfan
H A
HA
D000818:Animals; D000913:Antibodies, Protozoan / Q000032:analysis*; D003374:Coumarins; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes; D007891:Leishmania / Q000276:immunology*; D008722:Methods; D051379:Mice; D013861:Thiocyanates
D000913:Antibodies, Protozoan; D003374:Coumarins; D005452:Fluoresceins; D005456:Fluorescent Dyes; D013861:Thiocyanates; D016650:Fluorescein-5-isothiocyanate; C051029:7-amino-4-methylcoumarin-3-acetic acid