[An in vitro chemosensitivity test indicating regressive changes in the nucleus in cases of human ovarian cancer].
A simple in vitro sensitivity test of oncolytic drugs has been applied in 60 cases of human ovarian cancer. Tumor tissue blocks were minced with a razor blade, after which the cell clumps were poured into a tissue culture medium, containing an anticancer drug with a certain concentration, and incubated at 37 degrees C. Nine to 16 kinds of drugs were tested for each specimen. After incubation, the cell clumps were dispersed for providing smear specimens. Typical morphological changes appeared in the nucleus, characterized by karyorrhexis and karyopyknosis. The individual tumors displayed different sensitivities to the various drugs. The positive rate of alkylating agents ranged around 30%, and specimens examined after oncolytic treatment displayed low sensitivity.
Tokita
H
H
Division of Animal Research, Chiba Cancer Center Research Institute.
Tanaka
N
N
Ueno
T
T
Fujimoto
S
S
Sekiya
S
S
Takamizawa
H
H
D000970:Antineoplastic Agents / Q000494:pharmacology*; D002467:Cell Nucleus / Q000187:drug effects / Q000473:pathology*; D004354:Drug Screening Assays, Antitumor; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D010051:Ovarian Neoplasms / Q000473:pathology*
D000970:Antineoplastic Agents