title: A comparison of the evolutionary distribution of the two neuroendocrine markers, neurone-specific enolase and protein gene product 9.5.
abstract: One- and two-dimensional polyacrylamide gel electrophoresis followed by immunoblotting has been used to examine the phylogenetic distribution of the two neuronal and neuroendocrine proteins, neurone-specific enolase and protein gene product 9.5, in animal brains. A new immunoblotting procedure was used in which complex two-dimensional patterns of brain proteins were transferred to nitrocellulose paper simultaneously with the Coomassie Blue stain. This produced a copy of the blue spot pattern against which brown protein spots reacting in a specific antibody-immunoperoxidase procedure could be identified unequivocally. Extracts of human, bovine, sheep, rabbit, rat, guinea-pig, chicken, trout, and frog brains were examined. Proteins cross-reacting with antisera to the human forms of both proteins could be demonstrated in all species examined. This suggests that proteins corresponding to neurone-specific enolase and protein gene product 9.5 could have evolved at least 400 million years ago and have been highly conserved throughout evolution.
authors.forename: P
authors.initials: P
authors.lastname: Jackson
authors.forename: V M
authors.initials: VM
authors.lastname: Thomson
authors.forename: R J
authors.initials: RJ
authors.lastname: Thompson
mesh_terms: D000818:Animals; D001001:Anura; D005075:Biological Evolution; D001923:Brain Chemistry; D002417:Cattle; D002645:Chickens; D003101:Collodion; D004591:Electrophoresis, Polyacrylamide Gel; D006168:Guinea Pigs; D006801:Humans; D007124:Immunoenzyme Techniques; D007158:Immunologic Techniques; D009419:Nerve Tissue Proteins / Q000032:analysis*; D009490:Neurosecretory Systems / Q000032:analysis*; D010751:Phosphopyruvate Hydratase / Q000032:analysis*; D011817:Rabbits; D051381:Rats; D012756:Sheep; D014018:Tissue Distribution; D014337:Trout; D043222:Ubiquitin Thiolesterase
chemical_list: D009419:Nerve Tissue Proteins; D003101:Collodion; D043222:Ubiquitin Thiolesterase; D010751:Phosphopyruvate Hydratase