Effect of protein kinase C activation on cytoskeleton and cation transport in human erythrocytes. Reproduction of some membrane abnormalities revealed in essential hypertension.
Certain manifestations of alterations of membrane cytoskeleton, protein kinase C activity, and ion transport were revealed in erythrocytes of patients with essential hypertension: 1) the average volume of erythrocytes is reduced by 4%; 2) about 7% of the total number of erythrocytes is represented by cup-shaped forms compared with 1.5 to 3.0% in the control group; 3) basal phosphorylation of Band 4.9 protein is increased 1.6-fold to 1.8-fold; 4) activity of protein kinase C is increased by 60 to 70%; 5) the rate of proton electrochemical gradient (delta mu H+)-induced Na+-H+ exchange is increased twofold. Treatment of erythrocytes of healthy donors with protein kinase C activator (12-O-tetradecanoylphorbol-13-acetate) leads to similar but more marked changes in cell shape (17% of cup-shaped forms), volume reduction (by 7%), an increase of Band 4.9 protein phosphorylation (threefold), and an increase in the rate of Na+-H+ exchange (fourfold). Protein kinase activation does not modify Na+-Li+ exchange and slightly increases (by 20-50%) Na+-K+ pump activity, Na+-K+ cotransport, and the rate of 45Ca influx. It may be assumed that the increase of protein kinase C activity is one of the most probable molecular mechanisms conditioning abnormalities of the membrane skeleton and Na+-H+ exchange in primary hypertension.
Postnov
Y V
YV
Central Research Laboratory of the Ministry of Public Health of the USSR, Moscow.
Kravtsov
G M
GM
Orlov
S N
SN
Pokudin
N I
NI
Postnov
I Y
IY
Kotelevtsev
Y V
YV
D001692:Biological Transport; D001798:Blood Proteins / Q000378:metabolism; D002412:Cations / Q000097:blood*; D003599:Cytoskeleton / Q000502:physiology*; D004789:Enzyme Activation; D004910:Erythrocyte Membrane / Q000502:physiology*; D004911:Erythrocyte Volume; D004912:Erythrocytes / Q000378:metabolism / Q000473:pathology / Q000648:ultrastructure*; D005260:Female; D006801:Humans; D006973:Hypertension / Q000097:blood / Q000503:physiopathology*; D008297:Male; D008875:Middle Aged; D010766:Phosphorylation; D011493:Protein Kinase C / Q000097:blood*
D001798:Blood Proteins; D002412:Cations; D011493:Protein Kinase C