Method for growing primitive erythroid progenitors (BFU-E) from rat bone marrow.
A method for growing erythroid bursts from rat bone marrow cells is presented. The methylcellulose culture technique with rat spleen-conditioned medium was used. Successful growth of rat BFU-E was obtained with 20% fetal bovine serum when anemic rat plasma was used as the source of erythropoietin. Plating efficiency was 60 BFU-E per 2 x 10(5) nucleated cells seeded. The proliferative activity determined by cytosine arabinoside (Ara-C) suicide was about 22%. The effect of chronic hypertransfusion on erythroid colony formation is presented.
Basara
N
N
Institute for Medical Research, Beograd, Yugoslavia.
Biljanović-Paunović
L
L
Pavlović-Kentera
V
V
D000818:Animals; D001854:Bone Marrow Cells; D002449:Cell Aggregation; D003114:Colony-Forming Units Assay; D003470:Culture Media; D004900:Erythroblasts / Q000166:cytology* / Q000502:physiology; D004906:Erythrocyte Count; D004920:Erythropoiesis; D005260:Female; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000502:physiology; D051381:Rats; D011919:Rats, Inbred Strains
D003470:Culture Media