title: Sciatic nerve blood flow measured by laser Doppler flowmetry and [14C]iodoantipyrine.
abstract: Blood flow was examined in sciatic nerves of pentobarbital-anesthetized rats by means of laser Doppler flowmetry (LDF) and intravenous [14C]iodoantipyrine infusion. Continuous LDF signals demonstrated slow oscillations and acute, pressure-related changes in flow. The steady-state LDF signal was related linearly to nerve blood flow, as measured with [14C]iodoantipyrine, in intact nerves and nerves stripped of the epineurium. In 14 intact nerves, nerve blood flow averaged 0.27 +/- 0.03 (SE) ml X min-1 X g-1, whereas it averaged 0.13 +/- 0.01 in 5 stripped nerves. Autoradiographs of [3H]-nicotine-infused nerves and intra-arterial injection of 57Co-labeled microspheres demonstrated that flow was not uniform throughout the nerve cross section. The results indicate that LDF can be used to examine nerve blood flow in vivo, demonstrate a linear relation between the LDF signal and flow, and establish absolute values for blood flow in intact and stripped nerves of the anesthetized rat.
authors.forename: I
authors.initials: I
authors.lastname: Rundquist
authors.forename: Q R
authors.initials: QR
authors.lastname: Smith
authors.forename: M E
authors.initials: ME
authors.lastname: Michel
authors.forename: P
authors.initials: P
authors.lastname: Ask
authors.forename: P A
authors.initials: PA
authors.lastname: Oberg
authors.forename: S I
authors.initials: SI
authors.lastname: Rapoport
mesh_terms: D000818:Animals; D000983:Antipyrine / Q000031:analogs & derivatives; D001345:Autoradiography; D001794:Blood Pressure; D002250:Carbon Radioisotopes; D003037:Cobalt Radioisotopes; D008297:Male; D008863:Microspheres; D009538:Nicotine; D051381:Rats; D012039:Regional Blood Flow; D012212:Rheology; D012584:Sciatic Nerve / Q000098:blood supply*; D014316:Tritium; D014463:Ultrasonography
chemical_list: D002250:Carbon Radioisotopes; D003037:Cobalt Radioisotopes; D014316:Tritium; D009538:Nicotine; D000983:Antipyrine; C008135:iodoantipyrine