[Histochemical analysis of uterine cervical adenocarcinoma with reference to mucosubstances and distribution of CEA, CA125 and CA19-9].
Epithelial mucin and immunohistochemical localization of carbohydrate antigens (CA125, CA19-9) in normal endocervical glands and adenocarcinoma of uterine cervix were examined histochemically. The materials were obtained from 33 surgical cases with adenocarcinoma and 9 control cases. Serial sections were examined by the following procedures: 1) Digestive PAS reaction, 2) High iron diamine-Alcian blue pH 2.5 staining, 3) Modification PAS and Thionin Schiff reaction to differentiate sialic acid, 4) Immuno-peroxidase method. Histologically, these adenocarcinomas were classified into four subtypes, endocervical, intestinal, endometrioid and serous adenocarcinoma. Endocervical adenocarcinomas were differentiated into the endocervical-intestinal group and serous-endometrioid group by mucin profiles and distribution of carbohydrate antigens. In endocervical and intestinal types, sialomucin was predominant, this mucin being characterized by O-acetylated sialic acid. Immunohistochemically, CA19-9, CA125 and CEA were localized in this group. The latter group of tumors were serous and endometrioid adenocarcinomas, characterized by surface coat type sulphomucin and non-acetylated sialic acid, but CA19-9 and CA125 were not detected in these cases. Adenoma malignum was classified in endocervical type adenocarcinoma.
Maruyama
C
C
Department of Pathology, Kanazawa Medical University, Ishikawa.
Yamamichi
N
N
Konishi
F
F
D000230:Adenocarcinoma / Q000032:analysis / Q000145:classification / Q000276:immunology*; D000328:Adult; D000368:Aged; D000951:Antigens, Neoplasm / Q000032:analysis*; D015295:Antigens, Tumor-Associated, Carbohydrate; D002272:Carcinoembryonic Antigen / Q000032:analysis*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D009077:Mucins / Q000032:analysis*; D002583:Uterine Cervical Neoplasms / Q000032:analysis / Q000145:classification / Q000276:immunology*
D000951:Antigens, Neoplasm; D015295:Antigens, Tumor-Associated, Carbohydrate; D002272:Carcinoembryonic Antigen; D009077:Mucins