title: Generation of transducing particles in Staphylococcus aureus.
abstract: Transduction of plasmid pC194 and bacteriophage phi 11de varied inversely with the multiplicity of infection. As the multiplicity of infection decreased from 10(-1) to 10(-5) PFU/CFU, the transduction frequency of pC194 increased 10(4)-fold; the transduction frequency of phi 11de increased 300-fold with a 100-fold decrease in multiplicity of infection. Physical and genetic analysis of the transduced DNA showed that pC194 resided in the phage particle as a random, circularly permuted linear concatemer. In DNA prepared from phage that cotransduced pC194 and phi 11de, pC194 resided in the transducing phage primarily as a linear multimer of 15.8 kilobases, or about 5.4 pC194 monomers. The pC194 multimer was randomly inserted into the phi 11 genome.
authors.forename: D W
authors.initials: DW
authors.lastname: Dyer
authors.forename: M I
authors.initials: MI
authors.lastname: Rock
authors.forename: C Y
authors.initials: CY
authors.lastname: Lee
authors.forename: J J
authors.initials: JJ
authors.lastname: Iandolo
mesh_terms: D004279:DNA, Viral; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D013204:Staphylococcus Phages / Q000235:genetics / Q000528:radiation effects; D013211:Staphylococcus aureus / Q000235:genetics* / Q000648:ultrastructure; D014161:Transduction, Genetic; D014466:Ultraviolet Rays
chemical_list: D004279:DNA, Viral