title: Phosphorus-31 NMR as a probe for phosphoproteins.
abstract: Nuclear magnetic resonance methodology continues to advance such that phosphorus-31 NMR experiments can be profitably applied to elucidate some aspects of proteins which are covalently phosphorylated. This review introduces NMR spectral parameters pertinent to using phosphorus-31 NMR for investigation of structure and dynamics. The techniques of two-dimensional NMR, solid state NMR, and isotopic substitution are also introduced. Characteristics of phosphorylated amino acids and peptides, as revealed by phosphorus-31 NMR, are described. Studies of phosphorylated containing phosphomonoesters, phosphoramidates, acyl phosphates, and disubstituted phosphorus bridges are discussed. Among these phosphoproteins are several examples where phosphorus residues evidently play a role as polyelectrolytes, in enzyme catalysis, and in regulation of protein function.
authors.forename: T L
authors.initials: TL
authors.lastname: James
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002364:Caseins / Q000032:analysis; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003804:Dentin / Q000032:analysis; D005949:Glucose Oxidase / Q000378:metabolism; D006458:Hemolymph / Q000032:analysis; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D009124:Muscle Proteins / Q000032:analysis; D010047:Ovalbumin / Q000032:analysis; D010435:Pepsinogens / Q000032:analysis; D010455:Peptides / Q000032:analysis; D010733:Phosphoglucomutase / Q000032:analysis; D010750:Phosphoproteins / Q000032:analysis*; D010759:Phosphorus Isotopes; D010762:Phosphorylase a / Q000032:analysis; D010774:Phosvitin / Q000032:analysis; D012463:Saliva / Q000032:analysis; D013389:Succinate-CoA Ligases / Q000378:metabolism
chemical_list: D000596:Amino Acids; D002364:Caseins; D009124:Muscle Proteins; D010435:Pepsinogens; D010455:Peptides; D010750:Phosphoproteins; D010759:Phosphorus Isotopes; D010047:Ovalbumin; D010774:Phosvitin; D005949:Glucose Oxidase; D010762:Phosphorylase a; D000469:Alkaline Phosphatase; D010733:Phosphoglucomutase; D013389:Succinate-CoA Ligases