Thymus-derived macrophages in long-term culture: release of IL-1, stimulation of MLR and expression of tumoricidal activity.
The present report examines the behaviour of a slowly proliferating pure population of thymus-derived macrophages in long-term culture, regarding their ability to secrete interleukin-1 (IL-1) and prostaglandin E2 (PGE2), to stimulate a mixed leucocyte reaction (MLR) and to lyse tumour cells in vitro. Following stimulation with LPS, high levels of IL-1 were released to the medium. IL-1 release was significantly augmented by the addition of indomethacin during stimulation. Thymus-derived macrophages constitutively secreted significant levels of PGE2. These cells served as excellent stimulators in a one-way MLR, substantiating the claim that pure populations of macrophages effectively stimulate an allogeneic response in vitro. Thymus-derived macrophages showed tumoricidal activity following activation with either high concentrations of LPS or suboptimal concentrations of LPS and T-cell lymphokine. These findings portray a close interrelationship and reciprocal regulation between thymus-derived macrophages and T lymphocytes.
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D000818:Animals; D002478:Cells, Cultured; D003602:Cytotoxicity, Immunologic; D015232:Dinoprostone; D005260:Female; D005354:Fibrosarcoma / Q000473:pathology*; D007375:Interleukin-1 / Q000502:physiology*; D007959:Lymphocyte Culture Test, Mixed; D008262:Macrophage Activation; D008264:Macrophages / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D011458:Prostaglandins E / Q000378:metabolism; D013950:Thymus Gland / Q000276:immunology*
D007375:Interleukin-1; D011458:Prostaglandins E; D015232:Dinoprostone