Ionic strength-dependent proteolytic activation of protein kinase C by trypsin-like protease.
Protein kinase C, reversibly bound to rat liver plasma membrane through Ca2+, was activated by endogenous trypsin-like protease in an ionic strength-dependent manner. In an attempt to understand the reaction mechanism, the EGTA-extracted protein kinase C and the trypsin-like protease (Tanaka, K. et al. (1986) J. Biol. Chem. 261, 2610-2615) were separately purified from plasma membrane. In the reaction system using these purified enzymes, increasing the ionic strength with NaCl (140-210 mM) effectively enhanced the proteolytic activation of the protein kinase C in the presence of Ca2+ and phospholipid. These results suggest that ionic strength is an important factor for the proteolytic activation of membrane-bound rat liver protein kinase C.
Hashimoto
E
E
Department of Biochemistry, Fukui Medical School.
Mizuta
K
K
Sakanoue
Y
Y
Yamamura
H
H
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002462:Cell Membrane / Q000201:enzymology; D004533:Egtazic Acid; D004789:Enzyme Activation; D008099:Liver / Q000201:enzymology; D009994:Osmolar Concentration; D011493:Protein Kinase C / Q000302:isolation & purification / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D014357:Trypsin / Q000378:metabolism*
D004533:Egtazic Acid; D011493:Protein Kinase C; D014357:Trypsin; D002118:Calcium