Biological effects of gamma-interferon on human tumor cells: quantity and affinity of cell membrane receptors for gamma-IFN in relation to growth inhibition and induction of HLA-DR expression.
A large difference in the number of gamma-IFN receptors was found on a variety of human tumor cell lines with a range of 0.4 to 15 X 10(3) binding sites/cell. The receptor number did not correlate with the potential responsiveness of the cells to gamma-IFN, in regard to either growth inhibition or induction of HLA-DR expression, two independently regulated gamma-IFN effects. However, with respect to HLA-DR expression it was found that the gamma-IFN sensitivity of inducible cell lines depended on the number of receptors, so that with increasing number of receptors present on these cells lower doses of gamma-IFN were required for induction of the gamma-IFN effect.
Ucer
U
U
Bartsch
H
H
Scheurich
P
P
Pfizenmaier
K
K
D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D003110:Colonic Neoplasms / Q000473:pathology; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II / Q000032:analysis*; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism / Q000494:pharmacology*; D007938:Leukemia / Q000473:pathology; D009369:Neoplasms / Q000032:analysis* / Q000276:immunology; D011971:Receptors, Immunologic / Q000032:analysis*; D017471:Receptors, Interferon
D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II; D011971:Receptors, Immunologic; D017471:Receptors, Interferon; D007371:Interferon-gamma