Induction of tolerance to urushiol by epicutaneous application of this hapten on dinitrofluorobenzene-treated skin.
The application of a sensitizing dose of urushiol on a dinitrofluorobenzene (DNFB)-treated skin area significantly diminished the intensity of the urushiol challenge test in guinea pigs. Furthermore, the animals which had been first exposed to urushiol through DNFB-treated skin failed to become sensitized in a second sensitization attempt even when painted on a previously untreated area. This tolerance is hapten-specific and may be reversed by treatment with cyclophosphamide (200 mg/kg) shortly before another contact sensitization attempt to urushiol. In a previous work, we have shown that most of the Langerhans cells present in the DNFB-treated skin area are ATPase-negative and that there exists a link between the membranous ATPase system and the formation of Langerhans cell granules. The latter seem to develop in the course of a mechanism of adsorptive pinocytosis during which ATPase activity "disappears." Thus we suggest that the "unavailability" of ATPase-negative Langerhans cells for adequate processing a second hapten may result from the incapacity of cells lacking their ATPase system to activate the intracellular events that depend on this system and that normally lead to sensitization.
Hanau
D
D
Stampf
J L
JL
Fabre
M
M
Grosshans
E
E
Benezra
C
C
D000251:Adenosine Triphosphatases / Q000032:analysis; D000818:Animals; D002396:Catechols / Q000633:toxicity*; D003877:Dermatitis, Contact / Q000209:etiology* / Q000276:immunology; D004139:Dinitrofluorobenzene; D005260:Female; D006168:Guinea Pigs; D007108:Immune Tolerance / Q000187:drug effects; D007801:Langerhans Cells / Q000187:drug effects / Q000201:enzymology / Q000276:immunology*; D009578:Nitrobenzenes; D010873:Pinocytosis; D010947:Plants, Toxic; D012867:Skin / Q000276:immunology; D012882:Skin Tests / Q000379:methods; D013997:Time Factors
D002396:Catechols; D009578:Nitrobenzenes; C003747:urushiol; D004139:Dinitrofluorobenzene; D000251:Adenosine Triphosphatases