Monocyte suppressor function in burns: T cell-monocyte interaction in mediating suppression.
Reduced in vitro T cell mitogen-induced transformation, low proportion of T cells and increased proportion of non-T cells were found in blood mononuclear cells of patients with severe burns 3-12 days after the injury. High spontaneous proliferation of non-T cells was observed and could be related mainly to the B cell fraction. Monocytes mediated suppression of mitogen-stimulated T cell proliferation. We further studied the role of monocytes in the enhanced suppressor activity of Con A-activated T cells and found that in this assay system, the patient's T cells mediated suppression in collaboration with monocytes. In vitro, increased suppressor function was probably the result of in vivo stimulation of inhibitory activity ascribed to both monocytes and T cells of patients. Addition of indomethacin to cell cultures markedly reduced suppression of lymphocyte proliferation. Less significant reduction was noted when the patient's T cells were activated in vitro by Con A. Adjuvant treatment of burn patients with indomethacin may play a role in alleviating suppression of immune response in these patients.
Lahat
N
N
Hornstein
L
L
Moscona
A R
AR
Kalderon
N
N
Pollack
S
S
D000293:Adolescent; D000328:Adult; D000368:Aged; D002056:Burns / Q000276:immunology*; D002450:Cell Communication; D003208:Concanavalin A / Q000494:pharmacology; D005260:Female; D006801:Humans; D007108:Immune Tolerance; D007958:Leukocyte Count; D008213:Lymphocyte Activation; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000276:immunology* / Q000502:physiology; D013601:T-Lymphocytes / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology* / Q000502:physiology
D003208:Concanavalin A