Phorbol esters enhance attachment of NIH/3T3 cells to laminin and type IV collagen substrates.
The effect of phorbol esters on the adhesive properties of NIH/3T3 mouse fibroblasts was investigated using plastic substrates precoated with the extracellular matrix proteins fibronectin, collagen, and laminin. Treatment with phorbol 12-myristate 13-acetate (PMA) enhanced NIH/3T3 cell attachment to laminin and type IV collagen substrates but had little or no effect on attachment to fibronectin and type I collagen substrates. The effect of PMA in enhancing cell attachment to laminin and type IV collagen substrates was dose dependent between 10(-9) and 10(-7) M. PMA was effective as early as 30 min; the effect reached a maximum at 2 h and decreased gradually. Phorbol 12, 13-dibenzoate and phorbol 12, 13-diacetate were effective but to a lesser extent and phorbol 12-myristate and phorbol 13-acetate showed little or no effect. These results suggest that PMA may enhance NIH/3T3 cell adhesion through effects on laminin and type IV collagen receptors. Retinoic acid, which itself requires at least 6 h to show an effect on attachment, did not have any effect on cell attachment in 2 h and, if anything, slightly inhibited PMA-enhanced cell attachment to laminin and type IV collagen substrates.
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Differentiation Control Section, National Cancer Institute, Bethesda, Maryland 20892.
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D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects*; D002460:Cell Line; D003094:Collagen; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D007797:Laminin; D051379:Mice; D010703:Phorbol Esters / Q000494:pharmacology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014212:Tretinoin / Q000494:pharmacology
D007797:Laminin; D010703:Phorbol Esters; D014212:Tretinoin; D003094:Collagen; D013755:Tetradecanoylphorbol Acetate