[Glycine-dependent cryotransformation of Bacillus cereus by plasmid DNA].
A new technique for transformation of naturally noncompetent strains of Bac. cereus is proposed. Penetration of the DNA into recipient cells is based on two-step effect. At the first step of the process bacilli are affected by glycine in the early logarithmic phase of growth of the common periodic culture. At the second step the mixed DNA and recipient cells are frozen-thawed. The process permits the transforming DNA penetration via the outer membrane layer of the recipient cells having the affected permeability under the conditions of keeping bacillar recipient cells intact. The efficiency of transformation of Bac. cereus by the plasmids pUB110 and pBC16 DNA by the proposed technique is 1.10(4) and 3.10(3) of transformants per 1 mkg of the plasmid DNA.
Loginova
O G
OG
Ditiatkin
S Ia
SIa
D001409:Bacillus cereus / Q000235:genetics*; D003470:Culture Media; D004269:DNA, Bacterial / Q000235:genetics*; D005615:Freezing; D005998:Glycine; D010957:Plasmids; D014169:Transformation, Bacterial
D003470:Culture Media; D004269:DNA, Bacterial; D005998:Glycine
Glitsinzavisimaia kriotransformatsiia Bacillus cereus plazmidnoĭ DNK.