Interaction of a hydrophobic model peptide and its fatty acid derivative with lipid vesicles.
The interaction of a peptide [Lys-Phe-Phe-Phe-Ile-Ile-Trp-OCH3] and its fatty acid derivatives [Lys-(epsilon-palmitoyl)-Phe-Phe-Phe-Ile-Ile-Trp-OCH3 and Lys-(epsilon-12-(9-anthroyloxy)stearic acid)-Phe-Phe-Phe-Ile-Ile-OCH3] with model membranes was investigated by fluorescence spectroscopy. The emission characteristics of the Trp fluorophore indicated that only the peptide with the fatty acid chain is associated with lipid vesicles. Quenching experiments with spin probes suggest an orientation for the fatty acylated peptide wherein the fatty acid chain is perpendicular to the bilayer surface and the peptide chain parallel to the bilayer surface.
Joseph
M
M
Centre for Cellular and Molecular Biology, Hyderabad, India.
Nagaraj
R
R
D000215:Acylation; D003497:Cyclic N-Oxides / Q000494:pharmacology; D005456:Fluorescent Dyes; D008051:Lipid Bilayers / Q000378:metabolism; D008081:Liposomes / Q000378:metabolism*; D009842:Oligopeptides / Q000378:metabolism*; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013113:Spin Labels; D013229:Stearic Acids / Q000378:metabolism*
D003497:Cyclic N-Oxides; D005456:Fluorescent Dyes; D008051:Lipid Bilayers; D008081:Liposomes; D009842:Oligopeptides; D010169:Palmitic Acids; D013113:Spin Labels; D013229:Stearic Acids; C026653:12-(9-anthroyloxy)stearic acid; C057346:lysyl-phenylalanyl-phenylalanyl-phenylalanyl-isoleucyl-isoleucyl-tryptophyl methyl ester; C037404:12-doxylstearic acid; C005072:5-doxylstearic acid; D019308:Palmitic Acid; C032363:16-nitroxystearic acid