Production of a polyspecific human monoclonal antibody reacting with an epidermal antigen.
A clone of cells secreting an antibody to an epidermal antigen was generated from a patient with a blistering skin lesion. Although produced by fusion of human lymphocytes to a HAT-sensitive myeloma, this clone of cells did not have characteristics of a hybridoma. A true hybridoma was produced by fusion of this clone to a HATr/ouabain(r) myeloma line. The IgM antibody secreted by this clone reacted with the intercellular region of the epidermis of normal human skin in a manner similar to pemphigus autoantibodies. In addition, in normal human kidney the antibody bound to glomeruli and tubules. It also reacted with an antigen present in the cytoplasm of a wide variety of cell lines including epithelial, lymphoid and myeloid types. No reaction was found with the surface of any of the cell lines, nor with DNA or phospholipid antigens. This monoclonal antibody may define an autoantibody specificity which mediates some autoimmune skin lesions. Its polyspecificity is reminiscent of some other human hybridoma autoantibodies, and its reaction with components of the kidney suggests an alternative pathology for renal disease in such patients.
Hohmann
A W
AW
Department of Clinical Immunology, Flinders Medical Centre, Adelaide, Australia.
Coleman
M
M
Comacchio
R M
RM
Skinner
J M
JM
Bradley
J
J
D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000918:Antibody Specificity; D000941:Antigens; D001323:Autoantibodies; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D012867:Skin / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000941:Antigens; D001323:Autoantibodies