Comparative enzymology of eleven acid hydrolases in cultured amniotic fluid cells, skin fibroblasts and embryonic lung fibroblasts, and the respective changes with the increasing age of the cell cultures.
Assay conditions were studied for eleven lysosomal enzymes (beta-D-galactosidase, alpha-D-mannosidase, beta-hexosaminidase, beta-D-glucuronidase, alpha-D-galactosidase, alpha-D-glucosidase, arylsulfatase, beta-D-glucosidase, alpha-L-fucosidase, alpha-D-neuraminidase and alpha-L-iduronidase) in cultured amniotic fluid cells (CAFC), cultured skin fibroblasts (CSF) and cultured embryonic lung fibroblasts (CELF), and the properties of the enzymes were compared among these cultured cells. In addition, changes in these enzymes from the three cell types were investigated between 4-6 earlier passages and 24-26 later passages. With the exception of alpha-D-glucosidase, alpha-D-neuraminidase and alpha-L-fucosidase, all enzymes assayed for the 4-6 earlier passages and the 24-26 later passages had the same Km values and the same pH optima, and were also unchanged with the increasing age of cell cultures, with regard to their points. The specific activities of beta-D-glucuronidase, arylsulfatase, alpha-D-glucosidase and beta-D-glucosidase for the 4-6 earlier passages increased significantly with development, though no change was observed with development in the specific activities of other enzymes. Variations were observed between the levels of these enzymes in the three cell types with the increasing age of cell cultures, such as increases in some, decreases in others and no change in still others.
Minami
R
R
Wagatsuma
K
K
Ishikawa
Y
Y
Watanabe
M
M
Nakao
T
T
Tsugawa
S
S
D000653:Amniotic Fluid / Q000166:cytology* / Q000201:enzymology; D001192:Arylsulfatases / Q000378:metabolism; D002478:Cells, Cultured; D004622:Embryo, Mammalian; D005347:Fibroblasts / Q000201:enzymology*; D005696:Galactosidases / Q000378:metabolism; D005959:Glucosidases / Q000378:metabolism; D005966:Glucuronidase / Q000378:metabolism; D006596:Hexosaminidases / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006867:Hydrolases / Q000378:metabolism*; D007068:Iduronidase / Q000378:metabolism; D008168:Lung; D008247:Lysosomes / Q000201:enzymology; D008361:Mannosidases / Q000378:metabolism; D009439:Neuraminidase / Q000378:metabolism; D012867:Skin; D013997:Time Factors; D005644:alpha-L-Fucosidase / Q000378:metabolism; D043323:alpha-Mannosidase; D001619:beta-N-Acetylhexosaminidases
D006867:Hydrolases; D001192:Arylsulfatases; D005696:Galactosidases; D005959:Glucosidases; D006596:Hexosaminidases; D008361:Mannosidases; D009439:Neuraminidase; D043323:alpha-Mannosidase; D005966:Glucuronidase; D005644:alpha-L-Fucosidase; D001619:beta-N-Acetylhexosaminidases; D007068:Iduronidase