title: Liver replacement with reduced size donor organs.
abstract: End stage liver disease in children can be treated with orthotopic liver transplantation (OLT). Nevertheless, the expansion of this therapy in Europe has been limited because of the shortage of appropriate size-matched donors. One possible technical solution is the OLT of a liver graft previously reduced in size by in situ resection preceding the harvesting procedure. To study the impact of this technique we examined two different operative procedures performed on Landrace pigs. Group 1 consisted of 20 standard donor/recipient weight matched OLT. In group 2, 15 OLT were performed using right lateral and medial lobes (55% of the original donor liver). The donor/recipient weight ratio in the group was 2:1. Cold ischemia times were 90 +/- 16 min for group 1 and 98 +/- 9 min for group 2. It is emphasized that in contrast to all the other reports using resected liver grafts for OLT, the donor resection in our study was always performed in situ under normothermic conditions, preceding the harvesting procedure. This was designed to reduce the cold ischemia time. No significant technical problems were encountered. The biochemical results of group 2 pigs compared to group 1 demonstrate an analogous, postoperative course. This might be explained by regenerative stimuli acting on the resected liver tissues and enhancing their metabolic function. These data support the conclusion that resected adult donor liver grafts may be used for pediatric transplant recipients.
authors.affiliation: Department of Surgery, Catholic University of Rome, Italy.
authors.forename: I R
authors.initials: IR
authors.lastname: Marino
authors.forename: G
authors.initials: G
authors.lastname: De Luca
authors.forename: S
authors.initials: S
authors.lastname: Celli
authors.forename: E
authors.initials: E
authors.lastname: Santini
authors.forename: M M
authors.initials: MM
authors.lastname: Napolitano
authors.forename: D
authors.initials: D
authors.lastname: Kahn
authors.forename: L
authors.initials: L
authors.lastname: Perrelli
authors.forename: F
authors.initials: F
authors.lastname: Crucitti
mesh_terms: D000818:Animals; D005069:Evaluation Studies as Topic; D005260:Female; D008099:Liver / Q000033:anatomy & histology / Q000601:surgery; D016031:Liver Transplantation / Q000379:methods* / Q000473:pathology / Q000502:physiology; D009929:Organ Size; D013552:Swine