Expression of a leukemia-associated antigen (CAMAL) in four myeloid leukemia cell lines.
We have previously described the detection and partial characterization of a common myelogenous leukemia-associated antigen (CAMAL), in CGL and ANLL patients. Both polyclonal and monoclonal (CAMAL-1) antibodies have been raised to p70 (CAMAL) and have been shown to react with both p70 and myeloid leukemia cell preparations. p70 (CAMAL) has been shown to be a monomeric protein of Mr 70,000 and pI 7.2 and was also detectable in the myeloid leukemia cell lines HL60, KG1, K562 and U937, but not in the lymphocytic cell lines Molt-4, Hut-78 and CEM by immunoprecipitation from iodinated cell samples. Using [35S] methionine-labeled cell lines and immunoprecipitation, we have demonstrated the constitutive expression of p70 as well as a major component at p58 and a number at lower molecular weights in the myeloid leukemia cell lines HL60, KG1, K562 and U937, but not in the lymphocytic leukemia cell lines Molt-4, Hut-78 and CEM. The implications of these observations are discussed.
Shipman
R C
RC
Department of Microbiology, University of British Columbia, Vancouver, Canada.
Levy
J G
JG
D000911:Antibodies, Monoclonal; D000937:Antigen-Antibody Reactions; D015214:Antigens, Differentiation, Myelomonocytic / Q000276:immunology / Q000302:isolation & purification*; D014408:Biomarkers, Tumor / Q000302:isolation & purification*; D002458:Cell Fractionation; D002460:Cell Line; D006801:Humans; D007457:Iodine Radioisotopes; D007951:Leukemia, Myeloid / Q000378:metabolism*; D008715:Methionine; D008970:Molecular Weight; D011233:Precipitin Tests; D013462:Sulfur Radioisotopes
D000911:Antibodies, Monoclonal; D015214:Antigens, Differentiation, Myelomonocytic; D014408:Biomarkers, Tumor; D007457:Iodine Radioisotopes; D013462:Sulfur Radioisotopes; C061798:common myelogenous leukemia-associated antigen; D008715:Methionine