title: Methotrexate and bone marrow metaphases.
abstract: The efficacy of a methotrexate (MTX) block/thymidine release synchronization technique has been assessed in bone marrow cultures from patients with acute nonlymphocytic leukemia and myelodysplasia. In contrast to cultures of stimulated lymphocytes from normal individuals, no improvement in mitotic index (MI) or metaphase quality could be detected using this technique. Demonstration of an unchanged level of division in bone marrow cultures in the presence of MTX suggests that the technique is unsuitable for synchronization purposes in this tissue. The influence of preincubation prior to MTX exposure and duration of exposure to colcemid on MI and metaphase quality have also been examined.
authors.affiliation: Centre for Human Genetics, Children's Hospital, Sheffield, England.
authors.forename: J J
authors.initials: JJ
authors.lastname: Cunningham
authors.forename: A M
authors.initials: AM
authors.lastname: Potter
authors.forename: A E
authors.initials: AE
authors.lastname: Watmore
authors.forename: D A
authors.initials: DA
authors.lastname: Winfield
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001853:Bone Marrow / Q000187:drug effects / Q000473:pathology / Q000648:ultrastructure*; D002478:Cells, Cultured; D002871:Chromosome Banding; D002878:Chromosomes, Human, Pair 1; D005260:Female; D006801:Humans; D007621:Karyotyping; D007951:Leukemia, Myeloid / Q000235:genetics* / Q000473:pathology; D015470:Leukemia, Myeloid, Acute / Q000235:genetics* / Q000473:pathology; D008297:Male; D008677:Metaphase / Q000187:drug effects*; D008727:Methotrexate / Q000494:pharmacology*; D008875:Middle Aged; D009190:Myelodysplastic Syndromes / Q000235:genetics* / Q000473:pathology
chemical_list: D008727:Methotrexate