Presence of antibodies to human immunodeficiency virus-1 in reference reagents of human origin in diagnostic kits.
We tested 54 reagents of human origin that were included in a number of diagnostic kits to be used as positive or negative control material, and for quality assurance, for the presence of antibodies to human immunodeficiency virus-1 (HIV-1). Of these, 12 (22%) reagents were found to give positive results for antibodies to HIV-1 by enzyme-linked immunosorbent assay and/or supplementary tests. These results suggest that some diagnostic reagents of human origin may be contaminated with HIV-1 and potentially may be infectious.
Brake
F A
FA
National Biological Standards Laboratory, Commonwealth Department of Community Services and Health, Parkville, Vic.
Maskill
W J
WJ
Howard
T S
TS
Breschkin
A M
AM
Gandhi
S S
SS
D000914:Antibodies, Viral / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D004866:Equipment Contamination; D005189:False Positive Reactions; D006678:HIV / Q000276:immunology*; D015483:HIV Antibodies; D006801:Humans; D011786:Quality Control; D011933:Reagent Kits, Diagnostic
D000914:Antibodies, Viral; D015483:HIV Antibodies; D011933:Reagent Kits, Diagnostic