Effects of buffer salt on the geminate recombination of photodissociated carboxyhemoglobin and its isolated subunits.
The geminate recombination of photodissociated carboxyhemoglobin (human adult) and its isolated subunits was studied by nanosecond laser photolysis. The rebinding kinetics were found not to depend upon the kind of buffer used or on pH but changed appreciably with the buffer concentration. The results are compared to oxygenation properties under the same conditions.
Fontaine
M P
MP
Laboratoire de Photophysique Moléculaire du CNRS, Université Paris-Sud, Orsay, France.
Lindqvist
L
L
D002021:Buffers; D002263:Carboxyhemoglobin / Q000032:analysis* / Q000528:radiation effects; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D007834:Lasers; D010782:Photolysis; D011487:Protein Conformation
D002021:Buffers; D002263:Carboxyhemoglobin