title: Effect of the homokaryotic or heterokaryotic state of the uvs-2 allele in Neurospora crassa on mitomycin C-induced killing and ad-3 mutation.
abstract: Mitomycin C (MC) was tested for its killing and mutagenic activities in the ad-3 forward-mutation test in Neurospora crassa. The test was conducted in 4 dikaryons of N. crassa in order to determine the effect of the uvs-2 allele, which causes a defect in nucleotide excision repair, on MC-induced killing and ad-3 mutation. These dikaryons were homokaryotic for uvs-2+ (H-12), homokaryotic for uvs-2 (H-59), and heterokaryotic for uvs-2/uvs-2+ (H-70 and H-71). MC induced killing and ad-3 mutation in H-12, but the presence of uvs-2 in the homokaryotic state (H-59) resulted in a great increase in the killing and mutagenic activities of MC. This increased sensitivity to MC-induced killing and mutation conferred by uvs-2 in the homokaryotic state (H-59 vs. H-12) is a different effect than that noted by others for a defect in nucleotide excision-repair in Escherichia coli and Salmonella typhimurium or in human cells. The dikaryons heterokaryotic for uvs-2/uvs-2+ had the same sensitivity to MC as H-12, indicating that for MC-induced killing and ad-3 mutation uvs-2 is recessive to uvs-2+.
authors.forename: H E
authors.initials: HE
authors.lastname: Brockman
authors.forename: F J
authors.initials: FJ
authors.lastname: de Serres
authors.forename: C Y
authors.initials: CY
authors.lastname: Hung
mesh_terms: D000225:Adenine; D004260:DNA Repair; D006579:Heterozygote; D006720:Homozygote; D016685:Mitomycin; D008937:Mitomycins / Q000633:toxicity*; D009152:Mutagenicity Tests; D009491:Neurospora / Q000235:genetics*; D009492:Neurospora crassa / Q000187:drug effects / Q000235:genetics* / Q000528:radiation effects; D014466:Ultraviolet Rays
chemical_list: D008937:Mitomycins; D016685:Mitomycin; D000225:Adenine