[The inhibitory effects of anti class II monoclonal antibodies against the proliferative response of autoreactive T-cell clone].
The role of human class II antigens in autologous mixed lymphocyte reaction (AMLR) was investigated. The AMLR between PBL as a responder and autologous EBV-transformed cells as a stimulator was inhibited by anti class II framework (7B6) and anti DR (DI-4) monoclonal antibodies. Among monoclonal antibodies specific to DQ antigens, only HKD12 showed an inhibitory effect on AMLR. Of the autoreactive T-cell clones generated by AMLR, I-3 was peculiar; its proliferative response to autologous cells was inhibited only by HKD12. The inhibitory effect of HKD12 against AMLR or I-3 clone appeared to be epitope-specific. First, the result of sequential coprecipitation assay revealed that HKD12 reacted only a part of DQ antigen. Second, HKD12 did not inhibit allogeneic MLR. Third, the proliferative response of the I-3 clone was not inhibited by 7B6 which exerted a strong inhibitory effect on the proliferative response of autoreactive T-cell clones other than I-3 as well as allogeneic MLR.
Hasegawa
H
H
Ikeda
H
H
Katagiri
M
M
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002999:Clone Cells; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D007959:Lymphocyte Culture Test, Mixed; D051379:Mice; D011863:Radioimmunoassay; D013601:T-Lymphocytes / Q000276:immunology*
D000911:Antibodies, Monoclonal; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II