Spleen cell populations in normal and tumor-bearing hamsters.
Spleen cell subpopulations from normal and tumor-bearing hamsters (TBH) were quantified using Petri dishes coated with specific antibodies, and by flow cytometric immunofluorescence analysis. The relative numbers of T cells (Thy + cells) decreased, by both methods, as a function of tumor growth, while the number of B cells (bearing surface Ig) increased. Cells without T or B markers (null cells) were more numerous in the spleen of TBH and a large number of them expressed the receptor for the Fc fragment of IgG. Splenic cells were also sorted according to their light-scattering properties, and electron microscopic analysis was performed on the sorted fractions. It showed the presence of secreting plasmocytes and activated macrophages in the spleen of TBH.
Merdrignac
G
G
Haddada
H
H
De Vaux Saint Cyr
C
C
Genetet
B
B
D000818:Animals; D002452:Cell Count; D006224:Cricetinae; D008214:Lymphocytes / Q000166:cytology / Q000276:immunology; D008647:Mesocricetus; D009374:Neoplasms, Experimental / Q000276:immunology / Q000473:pathology*; D011961:Receptors, Fc; D017452:Receptors, IgG; D013154:Spleen / Q000166:cytology* / Q000276:immunology
D011961:Receptors, Fc; D017452:Receptors, IgG