Lymphoma-vesicle interactions: vesicle adsorption, membrane fragmentation, and intermembrane protein transfer.
Sonicated dimyristoylphosphatidylcholine vesicles interact with cultured murine lymphoma (BL/VL3) to generate complexes of vesicle and cell membrane components. Cell-free supernatants harvested after cell-vesicle incubations contain three distinct lipid species that can be separated by density gradient centrifugation. Analysis of protein and lipid composition and assays for cell and vesicle lumen contents reveal that the densest of the three lipid species comprises sealed plasma membrane fragments complexed with vesicles, while the least dense species is indistinguishable from pure phospholipid vesicles. The third, intermediate density species consists of topologically intact vesicles with associated plasma membrane proteins but without detectable cell lipids or cytoplasmic components. The membrane fragmentation and cell-to-vesicle protein transfer observed during lymphoma-vesicle incubations are examined as functions of cell and vesicle concentrations and incubation time.
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Department of Chemistry, Stanford University, California 94305.
Huestis
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D015060:1,2-Dipalmitoylphosphatidylcholine / Q000378:metabolism*; D000818:Animals; D002250:Carbon Radioisotopes; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D004134:Dimyristoylphosphatidylcholine / Q000378:metabolism*; D007700:Kinetics; D008223:Lymphoma / Q000378:metabolism*; D008563:Membrane Lipids / Q000378:metabolism*; D008565:Membrane Proteins / Q000302:isolation & purification / Q000378:metabolism*; D051379:Mice; D008970:Molecular Weight; D010713:Phosphatidylcholines
D002250:Carbon Radioisotopes; D008563:Membrane Lipids; D008565:Membrane Proteins; D010713:Phosphatidylcholines; D015060:1,2-Dipalmitoylphosphatidylcholine; D004134:Dimyristoylphosphatidylcholine