title: Effects of siderophores on the growth of Pseudomonas aeruginosa in human serum and transferrin.
abstract: A combination of the siderophores produced by Pseudomonas aeruginosa, pyochelin and pyoverdin, dramatically stimulates the growth of this bacterium in medium containing human transferrin. The amount of growth stimulation observed when each siderophore was added alone was only slightly less than the amount observed with the combination. Siderophore-defective mutants of strain PAO1 were isolated to test the effects of siderophore production on growth in transferrin and human serum. The pyoverdin-proficient (Pvd+), pyochelin-deficient (Pch-) strain (IA5) grows just as well as the parent (PAO1), which produces both siderophores. On the other hand, the Pvd- Pch+ strain (211-5) has severely retarded growth, similar to that demonstrated by a mutant lacking production of both siderophores (IA1), but has an accelerated log phase compared with strain IA1 at the later stages of the growth curve. However, the Pvd- Pch+ strain (211-5) had no observable advantage over the Pvd- Pch- strain, IA1, during incubation in human serum. The inability of P. aeruginosa strains to produce pyochelin in glucose-minimal medium may explain the poor growth of 211-5 in this medium and in human serum. The 211-5 strain grows much better than the IA1 strain in the medium that allows pyochelin synthesis, but it still does not grow as well as the Pvd+ Pch- strain (IA5). Therefore, pyoverdin appears to be the most important siderophore for growth in human serum.
authors.forename: R
authors.initials: R
authors.lastname: Ankenbauer
authors.forename: S
authors.initials: S
authors.lastname: Sriyosachati
authors.forename: C D
authors.initials: CD
authors.lastname: Cox
mesh_terms: D001798:Blood Proteins / Q000378:metabolism; D003470:Culture Media; D006801:Humans; D007501:Iron / Q000378:metabolism*; D007502:Iron Chelating Agents / Q000502:physiology*; D009842:Oligopeptides; D010636:Phenols / Q000502:physiology; D010860:Pigments, Biological / Q000502:physiology; D011550:Pseudomonas aeruginosa / Q000254:growth & development* / Q000378:metabolism; D017262:Siderophores; D013844:Thiazoles; D014168:Transferrin / Q000378:metabolism
chemical_list: D001798:Blood Proteins; D003470:Culture Media; D007502:Iron Chelating Agents; D009842:Oligopeptides; D010636:Phenols; D010860:Pigments, Biological; D017262:Siderophores; D013844:Thiazoles; D014168:Transferrin; C025316:pyochelin; C042453:pyoverdin; D007501:Iron