Cryo-electron microscopy of insect flight muscle thick filaments. An approach to dynamic electron microscope studies.
Suspensions of isolated insect flight muscle thick filaments were embedded in layers of vitreous ice and visualized in the electron microscope under liquid nitrogen conditions. The unfixed, unstained, unsupported and fully hydrated filaments were observed under various biochemical conditions. We demonstrate here the first successful application of this method to thick filaments, and show that this is a possible approach to following dynamic processes by rapid freezing and electron microscopy.
Menetret
J F
JF
Max Planck Institute for Medical Research, Department of Biophysics, Heidelberg, Federal Republic of Germany.
Hofmann
W
W
Lepault
J
J
D000818:Animals; D005426:Flight, Animal; D005615:Freezing; D007313:Insecta; D008722:Methods; D008854:Microscopy, Electron; D009132:Muscles / Q000648:ultrastructure*; D009218:Myosins / Q000648:ultrastructure*
D009218:Myosins