Transforming growth factor-beta 2: cDNA cloning and sequence analysis.
We have obtained a cDNA clone coding for human transforming growth factor (TGF)-beta 2. The clone was isolated from a tamoxifen-treated human prostatic adenocarcinoma cell line (PC-3) using oligonucleotide probes based on the partial amino acid sequence of purified TGF-beta 2. The cDNA sequence predicts that TGF-beta 2 is synthesized as a 442-amino-acid polypeptide precursor from which the mature 112-amino-acid TGF-beta 2 subunit is derived by proteolytic cleavage. The proteins coded for by the human TGF-beta 1 and TGF-beta 2 cDNAs show an overall homology of 41%. The mature and amino-terminal precursor regions show 71% and 31% homology, respectively. Northern blot analysis identified TGF-beta 2 transcripts of 4.1, 5.1, and 6.5 kb using mRNA from several different sources. Analysis of polyadenylated RNA from tamoxifen-treated PC-3 cells showed that these cells contain higher numbers of transcripts for TGF-beta 1 than for TGF-beta 2, although they produce more TGF-beta 2 protein than TGF-beta 1. This suggests that there is a post-transcriptional level of regulation for the production of these proteins.
Madisen
L
L
Oncogen, Seattle, WA 98121.
Webb
N R
NR
Rose
T M
TM
Marquardt
H
H
Ikeda
T
T
Twardzik
D
D
Seyedin
S
S
Purchio
A F
AF
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D010455:Peptides / Q000235:genetics*; D011498:Protein Precursors / Q000235:genetics; D011499:Protein Processing, Post-Translational; D012689:Sequence Homology, Nucleic Acid; D015291:Transforming Growth Factors; D014407:Tumor Cells, Cultured
D010455:Peptides; D011498:Protein Precursors; D015291:Transforming Growth Factors; D004247:DNA