[Role of calcium in the preparation and secretory activity of cells isolated from the gastric mucosa of rabbits].
The role of calcium in the preparation and the acid secretory activity of parietal cells was studied using cells isolated from rabbit gastric mucosa. The preparation of isolated cells was performed by enzymatic dissociation (collagenase) in the presence of EDTA; without EDTA, only isolated gastric glands were obtained. The acid secretory activity of parietal cells was determined by the 14C-aminopyrine accumulation method; the stimulation induced by histamine or isobutylmethylxanthine (IBMX) was not significantly affected by a reduction of extracellular Ca2+ level (20% diminution in a Ca2+-free medium). The carbachol induced stimulation was highly dependent upon the concentration of extracellular Ca2+: incubation of parietal cells in a Ca2+-free medium reduced the response to 100 microM carbachol by about 60%.
Magous
R
R
Léonard
A
A
Choquet
A
A
Baudière
B
B
Bali
J P
JP
D000818:Animals; D002118:Calcium / Q000494:pharmacology / Q000502:physiology*; D002217:Carbachol / Q000494:pharmacology; D002469:Cell Separation; D003470:Culture Media; D004492:Edetic Acid / Q000494:pharmacology; D005110:Extracellular Space; D006632:Histamine / Q000494:pharmacology; D066298:In Vitro Techniques; D010295:Parietal Cells, Gastric / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D011817:Rabbits
D003470:Culture Media; D006632:Histamine; D002217:Carbachol; D004492:Edetic Acid; D002118:Calcium
Rôle du calcium dans la préparation et l'activité sécrétoire de cellules isolées de la muqueuse gastrique de Lapin.