[Antigenicity test of cefodizime sodium].
The antigenicity studies of a beta-lactam antibiotic, cefodizime sodium (THR-221), were examined in mice and guinea pigs and the following results were obtained. 1) In passive cutaneous anaphylaxis (PCA) with sera from BALB/c or C3H/He mice, a slight PCA reaction was observed only in BALB/c mice by using THR-221-ovalbumin (OVA) plus Freund's complete adjuvant (FCA) as the immunogen and THR-221 itself as the eliciting antigen. 2) Immunological cross-reactivity between THR-221 and CTX was present at the rat PCA reaction. 3) Antibodies against THR-221 were detected by PCA in guinea pigs. The anti-THR-221 antibodies gave PCA responses smaller than those of CMZ by using antibiotic plus FCA as the immunogen and the antibiotic-bovine serum albumin (BSA) as the eliciting antigen. 4) In the observation of active systemic anaphylaxis (ASA), no symptoms were observed in guinea pigs sensitized with THR-221 and provoked with THR-221-BSA. On the other hand, guinea pigs sensitized with THR-221 plus FCA by injecting THR-221-BSA showed fetal anaphylactic reactions. 5) The results of passive hemagglutination (PHA) also indicated that the PHA titer of THR-221 was lower than that of CMZ. 6) Protein binding property of THR-221 with human serum albumin was lower than other beta-lactam antibiotics. 7) Antibodies against THR-221-OVA by using THR-221-BSA as the eliciting antigen were detected in any tests above mentioned. It is concluded from these results that immunological activity of THR-221 was equivalent to the other beta-lactam antibiotics.
Takemoto
M
M
Daiichi Pure Chemicals Co. Ltd., Tokai Research Laboratories, Tokai-mura Ibaraki-ken, Japan.
Matsuo
K
K
Katoh
S
S
Yoshida
R
R
D000707:Anaphylaxis / Q000139:chemically induced; D000818:Animals; D000900:Anti-Bacterial Agents / Q000276:immunology; D000906:Antibodies / Q000032:analysis / Q000276:immunology; D000941:Antigens / Q000276:immunology*; D002439:Cefotaxime / Q000031:analogs & derivatives* / Q000276:immunology / Q000378:metabolism; D003429:Cross Reactions; D006168:Guinea Pigs; D006386:Hemagglutination Tests; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D010323:Passive Cutaneous Anaphylaxis; D011485:Protein Binding; D051381:Rats; D011919:Rats, Inbred Strains; D012709:Serum Albumin / Q000378:metabolism
D000900:Anti-Bacterial Agents; D000906:Antibodies; D000941:Antigens; D012709:Serum Albumin; D002439:Cefotaxime; C033356:cefodizime