title: Autoradiographic evidence of muscarinic cholinergic receptors in the corpora cavernosa penis of the rat.
abstract: Recent studies have questioned the role of acetylcholine in the physiology of penile erectile tissue. The responsiveness of penile erectile tissue to acetylcholine would depend, in part, on the presence of cholinergic receptors on the smooth muscle. The specific binding of [3H]quinuclidinyl benzilate (QNB) to cholinergic receptors in sections of penile crura of the rat was analyzed by in vitro neurotransmitter autoradiography. Silver grain density measurements indicated that muscarinic cholinergic receptor binding sites are located almost entirely over the corpora cavernosa penis. Virtually no specific [3H]QNB binding was present in the tunica albuginea or adjacent skeletal muscle tissue. Within the erectile tissue, specific binding occurred both over the columns of intrinsic smooth muscle which form the walls of the cavernous spaces and around the more distal branches of the penile arteries. The high concentration of muscarinic cholinergic receptors in the corpora cavernosa penis is consistent with the suggestion that acetylcholine has an important, albeit undefined role in the function of penile erectile tissue.
authors.affiliation: Department of Anatomy, University of New Mexico School of Medicine, Albuquerque 87131.
authors.forename: N
authors.initials: N
authors.lastname: Minorsky
authors.forename: D D
authors.initials: DD
authors.lastname: Savage
authors.forename: W G
authors.initials: WG
authors.lastname: Dail
mesh_terms: D000818:Animals; D001158:Arteries / Q000378:metabolism; D001345:Autoradiography; D008297:Male; D009130:Muscle, Smooth / Q000033:anatomy & histology / Q000378:metabolism; D010413:Penis / Q000033:anatomy & histology / Q000098:blood supply / Q000648:ultrastructure*; D011813:Quinuclidinyl Benzilate / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D011950:Receptors, Cholinergic / Q000378:metabolism*; D011976:Receptors, Muscarinic / Q000378:metabolism*; D014018:Tissue Distribution
chemical_list: D011950:Receptors, Cholinergic; D011976:Receptors, Muscarinic; D011813:Quinuclidinyl Benzilate