Enhanced nitrosourea cytotoxicity in cell culture by sodium butyrate.
In HeLa S3 cells, sodium butyrate was found to potentiate the cytotoxicity of chloroethylnitrosoureas and alkylating agents in vitro. Using a soft-agar colony-forming assay, 2.5 and 5.0 mM sodium butyrate pretreatment for 22 h increased the cell killing efficacy of both methyl- and chloroethylnitrosoureas by between 30 and 70%. The potentiation of cytotoxicity of bifunctional nitrogen mustards by butyrate was less than that of nitrosoureas, with a 15-30% increased cell kill at 5 mM butyrate. Sodium butyrate per se reduced plating efficiency and caused growth delay if residual levels (calculated at 100 microM for starting concentrations of 5 mM) were not removed by washing prior to plating.
Vu
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Moy
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Schein
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D002087:Butyrates / Q000494:pharmacology*; D020148:Butyric Acid; D002470:Cell Survival / Q000187:drug effects; D003114:Colony-Forming Units Assay; D004357:Drug Synergism; D005260:Female; D006367:HeLa Cells / Q000187:drug effects; D006801:Humans; D008130:Lomustine / Q000494:pharmacology; D015376:Nimustine; D009588:Nitrogen Mustard Compounds / Q000494:pharmacology; D009607:Nitrosourea Compounds / Q000494:pharmacology*; D010752:Phosphoramide Mustards / Q000494:pharmacology; D013311:Streptozocin / Q000031:analogs & derivatives / Q000494:pharmacology
D002087:Butyrates; D009588:Nitrogen Mustard Compounds; D009607:Nitrosourea Compounds; D010752:Phosphoramide Mustards; D015376:Nimustine; D020148:Butyric Acid; C007028:chlorozotocin; D013311:Streptozocin; D008130:Lomustine