Nucleotide sequence of a staphylococcal plasmid gene, vgb, encoding a hydrolase inactivating the B components of virginiamycin-like antibiotics.
The nucleotide sequence of a 1883 bp fragment isolated from a resistance plasmid harbored by a Staphylococcus aureus clinical isolate and carrying the gene, vgb, encoding a hydrolase inactivating the B components of virginiamycin family has been determined. The sequence contains one open reading frame which extends from the ATG codon at nt 641 to a TGA codon at nt 1537 and which potentially codes for a protein of 33.035 Da. This value is in agreement with the apparent size (33 kDa) of the protein observed, in minicell extracts. Inactivation of the B components of the virginiamycin antibiotics as well as resistance to these antibiotics were expressed in a virginiamycin sensitive mutant of Escherichia coli recipient containing the gene on a high copy number plasmid.
Allignet
J
J
Laboratoire des Staphylocoques et des Streptocoques (Unité des Antigèns Bactériens), Institut Pasteur, Paris, France.
Loncle
V
V
Mazodier
P
P
el Solh
N
N
D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000378:metabolism; D001483:Base Sequence; D005796:Genes; D005798:Genes, Bacterial; D006867:Hydrolases / Q000235:genetics*; D008969:Molecular Sequence Data; D011815:R Factors; D015183:Restriction Mapping; D013211:Staphylococcus aureus / Q000201:enzymology / Q000235:genetics*; D014769:Virginiamycin / Q000378:metabolism
D000900:Anti-Bacterial Agents; D014769:Virginiamycin; D006867:Hydrolases