title: Re-endothelialization stages at the microvascular anastomosis.
abstract: After microsurgical suturing, the wound healing process at the rat femoral artery anastomosis was studied with scanning electron microscopy. The re-endothelialization was divided into four successive stages, based on the development of a fibrin network and the rate of endothelial regrowth. Within 5 minutes of blood flow re-establishment the fibrin network formation started to cover the wound surface; it was completed by the 3rd or 4th day. The endothelial recovery started on the 2nd day and continued to the base of the suture by the 6th day. Re-endothelialization from the wound edge, which was formed between intact and injured endothelium toward the suture-restrained site, was a two-fold process: initially, a single endothelial film covered the anastomosis site, then, the singled endothelial sheet proliferated and thickened to complete the endothelialization. A better understanding of the stages involved in microvascular repair is beneficial in evaluating vascular recovery and its relationship to patency rates in microvascular surgery.
authors.affiliation: Department of Plastic and Reconstructive Surgery, Kinki University School of Medicine, Osaka, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Isogai
authors.forename: H
authors.initials: H
authors.lastname: Kamiishi
authors.forename: S
authors.initials: S
authors.lastname: Chichibu
mesh_terms: D000818:Animals; D001158:Arteries / Q000601:surgery*; D002465:Cell Movement; D004730:Endothelium, Vascular / Q000502:physiology*; D005337:Fibrin / Q000502:physiology; D008297:Male; D008855:Microscopy, Electron, Scanning; D008866:Microsurgery; D010974:Platelet Aggregation; D051381:Rats; D011921:Rats, Inbred WKY; D013997:Time Factors; D014945:Wound Healing
chemical_list: D005337:Fibrin