title: Identification of 19-hydroxy-progesterone in human placenta.
abstract: We have tentatively demonstrated the presence of a 19-hydroxylated C21 steroid, 19-hydroxy-progesterone, in normal human placenta. A 19-hydroxylated steroid such as 19-hydroxy-progesterone, if produced by the placenta, could serve as a precursor for such hypertensinogenic 19-nor-steroids as 19-nor-deoxycorticosterone and 19-nor-progesterone. Freshly delivered, homogenized placental tissue was extracted and subjected to thin layer chromatography. A steroid corresponding to standard 19-hydroxy-progesterone was subsequently purified in HPLC, where authentic 19-hydroxy-progesterone and the sample had the same retention time. The identity of the sample was further confirmed by repeat HPLC after acetylation and mass spectrometry. Our experiment indicates that 19-hydroxy-progesterone is present in term placental tissue, where it appears to be synthesized.
authors.affiliation: Evans Memorial Department of Clinical Research, University Hospital, Boston, MA 02118.
authors.forename: J C
authors.initials: JC
authors.lastname: Melby
authors.forename: M
authors.initials: M
authors.lastname: Holbrook
authors.forename: M E
authors.initials: ME
authors.lastname: Ehlers
authors.forename: K
authors.initials: K
authors.lastname: Kyei-Aboagye
authors.forename: K
authors.initials: K
authors.lastname: Edelin
authors.forename: F A
authors.initials: FA
authors.lastname: Bencsath
authors.forename: F
authors.initials: F
authors.lastname: Field
authors.forename: G T
authors.initials: GT
authors.lastname: Griffing
mesh_terms: D000107:Acetylation; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D005260:Female; D006801:Humans; D006908:Hydroxyprogesterones / Q000032:analysis*; D013058:Mass Spectrometry; D010920:Placenta / Q000032:analysis*; D011247:Pregnancy
chemical_list: D006908:Hydroxyprogesterones; C057411:19-hydroxyprogesterone