Deoxynojirimycin inhibits the formation of Glc3Man9GlcNAc2-PP-dolichol in intestinal epithelial cells in culture.
The lipid-linked oligosaccharides synthesized in the presence of the alpha-glucosidase inhibitors, 1-deoxynojirimycin (DJN) and N-methyl-1-deoxynojirimycin (MDJN), were compared in IEC-6 intestinal epithelial cells in culture. HPLC analysis of the oligosaccharides obtained before and after exhaustive jack bean alpha-mannosidase digestion indicates that control and MDJN-treated cells synthesize similar amounts of Glc3Man9GlcNAc2-PP-dolichol. In contrast, the formation of this compound is greatly reduced in DJN-treated cells, the major lipid-linked oligosaccharide found being Man9GlcNAc2-PP-dolichol. The decreased availability of the preferred donor for protein glycosylation may account for the impaired glycosylation and secretion of certain glycoproteins in the presence of DJN.
Romero
P A
PA
Friedlander
P
P
Herscovics
A
A
D017485:1-Deoxynojirimycin / Q000031:analogs & derivatives; D000818:Animals; D002460:Cell Line; D004848:Epithelium / Q000378:metabolism; D005944:Glucosamine / Q000031:analogs & derivatives / Q000494:pharmacology; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000187:drug effects; D008358:Mannose / Q000378:metabolism; D008361:Mannosidases / Q000494:pharmacology; D009844:Oligosaccharides / Q000378:metabolism; D011105:Polyisoprenyl Phosphate Sugars / Q000096:biosynthesis*; D051381:Rats; D043323:alpha-Mannosidase
D009844:Oligosaccharides; D011105:Polyisoprenyl Phosphate Sugars; D017485:1-Deoxynojirimycin; D008361:Mannosidases; D043323:alpha-Mannosidase; D005944:Glucosamine; D008358:Mannose; C033424:N-methyldeoxynojirimycin