title: Membrane crystals of Ca2+-ATPase in sarcoplasmic reticulum of developing muscle.
abstract: The vanadate-induced crystallization of Ca2+-ATPase was analyzed on sarcoplasmic reticulum vesicles isolated between 10 and 28 days of development from pectoralis muscles of chicken. After exposure to Na3VO4 in a Ca2+-free medium, Ca2+-ATPase crystals begin to appear on portions of the surface of a few vesicles, isolated at 18 days of development. Thereafter, the number of vesicles containing Ca2+-ATPase crystals rapidly increases and after 1 week of postnatal development (28 days), it reaches the adult level of about 30% of the vesicle population. These observations are discussed with reference to the mechanism of Ca2+-ATPase crystallization and the regulation of sarcoplasmic reticulum biosynthesis.
authors.forename: L
authors.initials: L
authors.lastname: Dux
mesh_terms: D000818:Animals; D000252:Calcium-Transporting ATPases / Q000378:metabolism*; D002642:Chick Embryo; D002645:Chickens; D003460:Crystallization; D008854:Microscopy, Electron; D008861:Microsomes / Q000201:enzymology; D024510:Muscle Development; D009132:Muscles / Q000196:embryology / Q000201:enzymology; D012519:Sarcoplasmic Reticulum / Q000187:drug effects / Q000201:enzymology* / Q000648:ultrastructure; D014638:Vanadates; D014639:Vanadium / Q000494:pharmacology
chemical_list: D014639:Vanadium; D014638:Vanadates; D000252:Calcium-Transporting ATPases