A genetic analysis of the alpha-glycerophosphate oxidase locus in Drosophila melanogaster.
The gene for alpha-glycerophosphate oxidase, the nuclear encoded mitochondrial enzyme of the alpha-glycerophosphate cycle (alpha GP); has been mapped in Drosophila melanogaster. Several interstitial deficiencies in region 50c-53AB of chromosome 2R were used to localize the structural gene to 52D2-5. In addition, mutations of alpha GPO were generated; alpha GPO mutants are viable yet flightless. Interactions of alpha GPO with alpha-glycerophosphate dehydrogenase (alpha GPDH), the cytoplasmic enzyme of the alpha GP cycle, were investigated through the synthesis of a series of alpha GPDHnull-alpha GPOnull double mutants. Of the six double null mutants constructed, four alpha GPDH-alpha GPO double nulls are viable and flightless. Two double mutants, however, exhibit an allelic-dependent synthetic lethal phenotype.
Davis
M B
MB
Section of Genetics and Development, Cornell University, Ithaca, New York 14853.
MacIntyre
R J
RJ
D000782:Aneuploidy; D000818:Animals; D002872:Chromosome Deletion; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D004331:Drosophila melanogaster / Q000201:enzymology / Q000235:genetics*; D005020:Ethyl Methanesulfonate; D005260:Female; D005786:Gene Expression Regulation; D005796:Genes; D005804:Genes, Lethal; D005816:Genetic Complementation Test; D005993:Glycerolphosphate Dehydrogenase / Q000235:genetics*; D008297:Male; D009154:Mutation
D005020:Ethyl Methanesulfonate; D005993:Glycerolphosphate Dehydrogenase