The heterogeneity of rat kidney lysosomes revealed by immunoelectron microscopic staining for cathepsins B and H.
The heterogeneity of lysosomes was studied by analyzing the immunostaining behavior of cathepsins B and H in rat kidney proximal tubule cells. Rat kidneys were fixed by perfusion and embedded in Lowicryl K4M. A protein A-gold technique was applied to serial sections and a double labeling technique to conventional sections. By analyzing the immunostaining behavior of cathepsins B and H in the same lysosomes which were cut into separate sections, four types of lysosomes were found: Type 1 positive for both proteinases; type 2 strongly positive for cathepsin B, but weakly or negative for cathepsin H; type 3 strongly positive for cathepsin H, but weakly or negative for cathepsin B; and type 4 negative for both proteinases. The double labeling by two different sizes of the protein A-gold probes showed these four types of lysosomes. The results indicate that there exists the lysosomal heterogeneity of the proteinase content in the kidney proximal tubule cells.
Yokota
S
S
Department of Anatomy, Yamanashi Medical School, Japan.
Kato
K
K
D000818:Animals; D002401:Cathepsin B / Q000032:analysis*; D056655:Cathepsin H; D002403:Cathepsins / Q000032:analysis*; D003546:Cysteine Endopeptidases; D006046:Gold; D007150:Immunohistochemistry; D007687:Kidney Tubules, Proximal / Q000201:enzymology* / Q000648:ultrastructure; D008247:Lysosomes / Q000201:enzymology* / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D051381:Rats; D011919:Rats, Inbred Strains
D006046:Gold; D002403:Cathepsins; D003546:Cysteine Endopeptidases; D002401:Cathepsin B; D056655:Cathepsin H; C534128:Ctsh protein, rat