Evaluation of a monoclonal antibody affinity purified antigen for zymodeme specific serological diagnosis of Trypanosoma cruzi infection.
Theoretically, serological assays with affinity purified marker antigens can allow strain-specific diagnosis even when parasites cannot be retrieved from an infected host. A Trypanosoma cruzi antigen was purified by affinity chromatography using a zymodeme (Z) 2 specific monoclonal antibody (2E2C11). An indirect enzyme-linked immunosorbent assay (ELISA) based on the purified antigen could discriminate between sera from rabbits immunized with T. cruzi zymodeme clones but could not discriminate between sera from mice infected with different zymodemes.
Schechter
M
M
Wolfson Molecular Biology Unit/Department of Medical Protozoology, London School of Hygiene and Tropical Medicine.
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D000953:Antigens, Protozoan / Q000302:isolation & purification*; D014355:Chagas Disease / Q000175:diagnosis*; D002846:Chromatography, Affinity; D004797:Enzyme-Linked Immunosorbent Assay; D051379:Mice; D011817:Rabbits; D014349:Trypanosoma cruzi / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan