Characterization of crystals of xylose isomerase from Streptomyces violaceoniger.
Crystals of the tetrameric xylose isomerase from Streptomyces violaceoniger have been examined by x-ray analysis. Octahedral crystals with a maximum dimension of 0.7 mm were grown from ammonium sulfate solution. They possess the symmetry of P4(1)2(1)2 or P4(3)2(1)2 space groups, which are crystallographically indistinguishable. The unit cell dimensions are a = b = 140 A and c = 134 A. There is one tetramer of molecular weight 160,000 per asymmetric unit. The crystals diffract to 2.2 A.
Glasfeld
A
A
Department of Chemistry, Massachusetts Institute of Technology, Cambridge 02139.
Farber
G K
GK
Ringe
D
D
Marcel
T
T
Drocourt
D
D
Tiraby
G
G
Petsko
G A
GA
D019747:Aldose-Ketose Isomerases; D002238:Carbohydrate Epimerases / Q000302:isolation & purification; D046911:Macromolecular Substances; D011487:Protein Conformation; D013302:Streptomyces / Q000201:enzymology*; D014961:X-Ray Diffraction / Q000379:methods
D046911:Macromolecular Substances; D002238:Carbohydrate Epimerases; D019747:Aldose-Ketose Isomerases; C023298:xylose isomerase