title: Potentiation of fibroblast spreading by extracellular matrix from fibroblasts derived from phenytoin-induced gingival overgrowth.
abstract: Cell attachment and spreading appear when a cell, on contact with an appropriate substratum, adheres and changes its shape and accommodates to the substratum. The transition from a non-spreading to a spreading state is a prerequisite for growth. Cell-free extracellular matrix (ECM) was produced by fibroblast-like cells from normal gingiva (N-ECM) and phenytoin-induced gingival overgrowth (PHT-ECM). The effect of the ECM on cell attachment and spreading of human gingival fibroblasts was studied in the presence of 2% serum. Within 30 min after seeding 40% of the normal fibroblast cells showed an advanced flattening on PHT-ECM-prepared dishes, compared with 10% on normal ECM-prepared dishes and 5% on uncoated plastic dishes. The results indicate that cells derived from PHT-induced gingival overgrowth produce an ECM with special properties, which could regulate cell functions such as cell attachment and spreading.
authors.affiliation: Department of Pedodontics, Karolinska Institute, Stockholm, Sweden.
authors.forename: T
authors.initials: T
authors.lastname: Modéer
authors.forename: G
authors.initials: G
authors.lastname: Dahllöf
authors.forename: P
authors.initials: P
authors.lastname: Otteskog
mesh_terms: D000293:Adolescent; D000328:Adult; D002448:Cell Adhesion; D002465:Cell Movement; D002478:Cells, Cultured; D002648:Child; D005109:Extracellular Matrix / Q000502:physiology*; D005347:Fibroblasts / Q000166:cytology* / Q000502:physiology; D005885:Gingival Hyperplasia / Q000473:pathology* / Q000503:physiopathology; D006801:Humans; D010672:Phenytoin / Q000009:adverse effects*
chemical_list: D010672:Phenytoin