Synaptic currents recorded from the dendritic field of an insect giant interneurone.
A method is described enabling the recording of synaptic currents from an isolated interganglionic interneurone in the central nervous system of the cockroach, Periplaneta americana, using an oil-gap recording system. The circuitry contains a Wheatstone bridge in which the preparation is the unknown resistance. Using a voltage-clamp technique, both excitatory and inhibitory postsynaptic currents together with currents induced by metabolic changes can be detected.
Mony
L
L
Laboratoire de Physiologie, UA CNRS No. 611 UER Médecine, Angers, France.
Hue
B
B
Tessier
J C
JC
D000669:Amplifiers, Electronic; D000818:Animals; D003058:Cockroaches / Q000502:physiology*; D003712:Dendrites / Q000502:physiology*; D004553:Electric Conductivity; D004558:Electric Stimulation; D005071:Evoked Potentials; D007395:Interneurons / Q000502:physiology*; D008564:Membrane Potentials; D008839:Microelectrodes; D010526:Periplaneta / Q000502:physiology*; D013569:Synapses / Q000502:physiology*