Quantitative secondary ion monitoring gas chromatography/mass spectrometry of diethylstilbestrol in bovine liver.
A procedure is described for the extraction of diethylstilbestrol (DES) from animal tissue for quantitative capillary gas chromatography/mass spectrometry (GC/MS). The procedure is based upon use of a strong anion exchange polystyrene divinylbenzene resin for sample purification. The recovery of DES from the resin clean up was 88% in the high parts per trillion (ppt) range. Criteria for identification of DES using selected ion monitoring (SIM) GC/MS are presented. Liquid chromatography/mass spectrometry (LC/MS) was used to investigate altered DES cis/trans ratios observed in biological extracts.
Covey
T
T
Maylin
G
G
Henion
J
J
D000818:Animals; D002417:Cattle; D002852:Chromatography, Ion Exchange / Q000379:methods; D004054:Diethylstilbestrol / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D007202:Indicators and Reagents; D008099:Liver / Q000032:analysis*; D008832:Microchemistry; D014316:Tritium
D007202:Indicators and Reagents; D014316:Tritium; D004054:Diethylstilbestrol