Interferon-beta 2 (BSF-2) mRNA is expressed in human monocytes.
We previously have reported the presence of interferon-beta 2 (IFN-beta 2) mRNA in PHA-stimulated human peripheral blood leukocytes (PBL), as well as in nonstimulated cells, although at a lower level. The IFN-beta 2 cloned from a leukocyte library appeared to be similar to that of the fibroblast IFN-beta 2 gene first described in fibroblasts. To assess the nature of the cell population in which the synthesis of IFN-beta 2 takes place, PBL were fractionated in adherent and nonadherent cells. The antiviral activity of the culture supernatants of adherent cells was characterized as the IFN-beta type by neutralization with polyclonal antibodies raised against purified fibroblast IFN-beta 2. IFN-beta 2 mRNA was observed in enriched monocyte populations and accumulated very rapidly, peaking at 2.5 h. RNA extracted from these cultures encoded in a reticulocyte lysate a protein immunoprecipitated by the anti-IFN-beta 2 antiserum. In addition, IFN-beta 2 secreted in monocyte supernatants also was immunoprecipitated by the specific antiserum and was able to compete with the fibroblast IFN-beta 2, suggesting a strong similarity between the fibroblast and monocyte proteins.
Sancéau
J
J
Unité 196 INSERM, Institut Curie, Section de Biologie, Paris, France.
Falcoff
R
R
Zilberstein
A
A
Béranger
F
F
Lebeau
J
J
Revel
M
M
Vaquero
C
C
D002448:Cell Adhesion; D005786:Gene Expression Regulation; D006801:Humans; D007370:Interferon Type I / Q000032:analysis / Q000235:genetics*; D009000:Monocytes / Q000032:analysis*; D009500:Neutralization Tests; D011233:Precipitin Tests; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics*
D007370:Interferon Type I; D012333:RNA, Messenger