Anti-GBM antibody-induced proteinuria in isolated perfused rat kidney.
The effect of anti-GBM antibody on protein excretion was studied in isolated rat kidneys perfused with 20 mg of sheep anti-rat GBM (experimental) or nonantibody sheep IgG (control). Six control kidneys excreted 176 +/- 31 micrograms/min of BSA initially, rising to 296 +/- 111 micrograms/min at 2 h. Fractional clearance of BSA rose from 0.51 to 1.70%. Eight experimental kidneys excreted 211 +/- 56 micrograms/min of BSA, increasing to 1,924 +/- 804 micrograms/min at 2 h. Fractional BSA clearance increased from 0.56 to 11.49%. After 60 min, BSA excretion in anti-GBM-perfused kidneys exceeded controls by a factor of 6.5-7.9 (P less than 0.05) and fractional BSA clearance exceeded controls by a factor of 5.8-7.1 (P less than 0.05). Studies with fluorescent markers indicated proteinuria to be of glomerular origin in antibody-perfused kidneys. There were no significant differences between anti-GBM-perfused and control kidneys in perfusion pressures, perfusate flow rates, urine flow rates, inulin clearance, or sodium reabsorption. Antibody to GBM can induce a marked increase in glomerular permeability to BSA and IgG without participation of other systemic humoral or cellular mediation systems.
Couser
W G
WG
Darby
C
C
Salant
D J
DJ
Adler
S
S
Stilmant
M M
MM
Lowenstein
L M
LM
D000818:Animals; D000906:Antibodies / Q000276:immunology*; D001485:Basement Membrane / Q000276:immunology; D005919:Glomerular Filtration Rate; D005921:Glomerulonephritis / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D007105:Immune Complex Diseases / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D007074:Immunoglobulin G / Q000276:immunology; D066298:In Vitro Techniques; D007668:Kidney / Q000378:metabolism / Q000648:ultrastructure; D007678:Kidney Glomerulus / Q000276:immunology* / Q000378:metabolism; D008854:Microscopy, Electron; D010477:Perfusion; D011507:Proteinuria / Q000276:immunology* / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012710:Serum Albumin, Bovine / Q000378:metabolism; D012756:Sheep / Q000276:immunology
D000906:Antibodies; D007074:Immunoglobulin G; D012710:Serum Albumin, Bovine