[Isolation and primary characteristics of elastase from a clinical strain of Pseudomonas aeruginosa].
The homogeneous preparation of elastase has been obtained from P. aeruginosa clinical strain. The molecular weight of the isolated enzyme is 33,000 daltons, and its isoelectric point is 6.8. Two media manufactured in this country (dialyzed bovine heart hydrolysate and a dried semisynthetic medium) ensuring good production of the enzyme have been proposed. The optimum time for the cultivation of the producer strain (30-40 hours) has been established. Elastase has been shown to be widely spread among P. aeruginosa clinical strains.
El-Bazza
Z E
ZE
Moroz
A F
AF
Glatman
L I
LI
Levdikova
G A
GA
Terekhov
A A
AA
D000818:Animals; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007106:Immune Sera / Q000302:isolation & purification; D005779:Immunodiffusion; D007525:Isoelectric Focusing; D010196:Pancreatic Elastase / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification*; D011550:Pseudomonas aeruginosa / Q000201:enzymology* / Q000302:isolation & purification; D011817:Rabbits
D007106:Immune Sera; D010196:Pancreatic Elastase
Poluchenie i pervichnaia kharakteristika élastazy iz klinicheskogo shtamma Pseudomonas aeruginosa.