title: 2-Mercaptoethylamine, a competitive inhibitor of spermidine synthase in mammalian cells.
abstract: Spermidine synthase from rat ventral prostate was inhibited by 2-mercaptoethylamine (MEA). Inhibition of spermidine synthase by MEA was competitive with respect to one of the substrates putrescine, but not competitive with respect to the other substrate decarboxylated S-adenosylmethionine. MEA markedly depressed spermidine and spermine contents in human erythroid leukemia K562 cells, suggesting that these changes resulted from the inhibitory effect of MEA on spermidine synthase in situ.
authors.affiliation: Department of Biochemistry, Mie University School of Medicine, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Hibasami
authors.forename: M
authors.initials: M
authors.lastname: Kawase
authors.forename: T
authors.initials: T
authors.lastname: Tsukada
authors.forename: S
authors.initials: S
authors.lastname: Maekawa
authors.forename: M
authors.initials: M
authors.lastname: Sakurai
authors.forename: K
authors.initials: K
authors.lastname: Nakashima
mesh_terms: D000818:Animals; D001667:Binding, Competitive; D002460:Cell Line; D003543:Cysteamine / Q000494:pharmacology*; D007700:Kinetics; D007951:Leukemia, Myeloid; D008297:Male; D011467:Prostate / Q000201:enzymology*; D051381:Rats; D011919:Rats, Inbred Strains; D011436:Spermidine Synthase / Q000037:antagonists & inhibitors* / Q000302:isolation & purification; D013097:Spermine Synthase / Q000302:isolation & purification / Q000378:metabolism; D014166:Transferases / Q000037:antagonists & inhibitors*
chemical_list: D003543:Cysteamine; D014166:Transferases; D011436:Spermidine Synthase; D013097:Spermine Synthase