Isolation of nuclei from melittin-destabilized cells.
Melittin, the hydrophobic polypeptide from bee venom, sufficiently destabilizes the plasma membrane of cultured cells to allow cell disruption in the absence of detergents with minimal homogenization. Nuclei are thus isolated in high yield with intact nuclear membranes and high transcriptional activity.
Smith
P J
PJ
Department of Biochemistry, University of Cape Town, Rondebosch, Republic of South Africa.
Friede
M H
MH
Scott
B J
BJ
von Holt
C
C
D001514:Bee Venoms / Q000494:pharmacology*; D002462:Cell Membrane / Q000187:drug effects*; D002467:Cell Nucleus; D006868:Hydrolysis; D004915:Leukemia, Erythroblastic, Acute / Q000378:metabolism; D007942:Leukemia, Experimental / Q000378:metabolism; D008555:Melitten / Q000494:pharmacology*; D008563:Membrane Lipids / Q000302:isolation & purification; D008565:Membrane Proteins / Q000302:isolation & purification; D009685:Nuclear Envelope / Q000187:drug effects; D012995:Solubility; D014158:Transcription, Genetic
D001514:Bee Venoms; D008563:Membrane Lipids; D008565:Membrane Proteins; D008555:Melitten