[Mitomycin C induced structural changes in heterochromatic regions of human chromosomes 1, 9, 16 and Y].
Aberrations and variations in the heterochromatic blocks of chromosomes 1, 9, 16 and Y were found under the influence of mitomycin C in cultured lymphocytes of peripheral human blood. Lymphocytes were cultured during 96 hours, mitomycin C in final concentration of 0.3 mkg/ml was present in the culture during the latest 24 hours of culturing. Different changes in the heterochromatic regions of chromosomes were found in approximately 30% of cells: in 6.3% of cells mitotic chiasmata were indicated. In 9.5% of cells isolocus breaks were observed in heterochromatic region of chromosome 1 in segment 1q11. In the latter case this may be a fragile site detected under the influence of mitomycin C on the lymphocytes.
Malygina
N A
NA
Remizova
O G
OG
Akuf'ev
A P
AP
D002478:Cells, Cultured; D002869:Chromosome Aberrations / Q000187:drug effects*; D002878:Chromosomes, Human, Pair 1; D002885:Chromosomes, Human, Pair 16; D002899:Chromosomes, Human, Pair 9; D006570:Heterochromatin / Q000187:drug effects*; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects; D016685:Mitomycin; D008937:Mitomycins / Q000633:toxicity*; D008938:Mitosis / Q000187:drug effects; D014998:Y Chromosome
D006570:Heterochromatin; D008937:Mitomycins; D016685:Mitomycin
Indutsirovannye mitomitsinom C strukturnye izmeneniia geterokhromaticheskikh raĭonov khromosom 1, 9, 16 i Y cheloveka.