2-Mercaptoethylamine, a competitive inhibitor of spermidine synthase in mammalian cells.
Spermidine synthase from rat ventral prostate was inhibited by 2-mercaptoethylamine (MEA). Inhibition of spermidine synthase by MEA was competitive with respect to one of the substrates putrescine, but not competitive with respect to the other substrate decarboxylated S-adenosylmethionine. MEA markedly depressed spermidine and spermine contents in human erythroid leukemia K562 cells, suggesting that these changes resulted from the inhibitory effect of MEA on spermidine synthase in situ.
Hibasami
H
H
Department of Biochemistry, Mie University School of Medicine, Japan.
Kawase
M
M
Tsukada
T
T
Maekawa
S
S
Sakurai
M
M
Nakashima
K
K
D000818:Animals; D001667:Binding, Competitive; D002460:Cell Line; D003543:Cysteamine / Q000494:pharmacology*; D007700:Kinetics; D007951:Leukemia, Myeloid; D008297:Male; D011467:Prostate / Q000201:enzymology*; D051381:Rats; D011919:Rats, Inbred Strains; D011436:Spermidine Synthase / Q000037:antagonists & inhibitors* / Q000302:isolation & purification; D013097:Spermine Synthase / Q000302:isolation & purification / Q000378:metabolism; D014166:Transferases / Q000037:antagonists & inhibitors*
D003543:Cysteamine; D014166:Transferases; D011436:Spermidine Synthase; D013097:Spermine Synthase