title: Sequence-specific chemical modification of double-stranded DNA with alkylating oligodeoxyribonucleotide derivatives.
abstract: Chemical modification of double-stranded (ds) DNA with alkylating oligodeoxynucleotide (oligo) derivatives, 5'-p(N-2-chloroethyl-N-methylamino) benzylamides of oligos, has been investigated. In contrast to relaxed plasmid DNAs, the superhelical molecules interact with the oligo derivatives and specific alkylation of the DNAs occurs at the regions complementary to the oligo reagents. Alkylating derivatives of oligocytidylates and pT(pCpT)6 react with corresponding homopyrimidine-homopurine tracts within ds DNA fragments due to triple helix formation.
authors.affiliation: Institute of Bioorganic Chemistry, Siberian Division of the Academy of Sciences of U.S.S.R., Novosibirsk.
authors.forename: V V
authors.initials: VV
authors.lastname: Vlassov
authors.forename: S A
authors.initials: SA
authors.lastname: Gaidamakov
authors.forename: V F
authors.initials: VF
authors.lastname: Zarytova
authors.forename: D G
authors.initials: DG
authors.lastname: Knorre
authors.forename: A S
authors.initials: AS
authors.lastname: Levina
authors.forename: A A
authors.initials: AA
authors.lastname: Nikonova
authors.forename: L M
authors.initials: LM
authors.lastname: Podust
authors.forename: O S
authors.initials: OS
authors.lastname: Fedorova
mesh_terms: D000478:Alkylation; D000818:Animals; D001483:Base Sequence; D055598:Chemical Phenomena; D002621:Chemistry; D004278:DNA, Superhelical; D015321:Gene Rearrangement; D005803:Genes, Immunoglobulin; D007147:Immunoglobulin Light Chains / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D009150:Mustard Compounds; D009838:Oligodeoxyribonucleotides; D010957:Plasmids
chemical_list: D004278:DNA, Superhelical; D007147:Immunoglobulin Light Chains; D009150:Mustard Compounds; D009838:Oligodeoxyribonucleotides