title: In vivo determinations of labelling index of metastatic colorectal carcinoma and normal colonic mucosa using intravenous infusions of bromodeoxyuridine.
abstract: In vivo determination of the labelling index of metastatic colon carcinoma was performed in 14 patients following intravenous infusion of bromodeoxyuridine (BrdU), at doses of 100 and 50 mg/M2. No toxicity was seen. Labelled cells were detected by an immunohistochemical technique using a monoclonal antibody to BrdU. Labelling was uniform and highly specific with good preservation of morphology. Labelling index values ranged from 15.0-40.6% for metastatic lesions and 3.6%-20% for normal colonic mucosa, with mean values of 24% and 11%, respectively. This represents a useful new method for in vivo labelling index determination in solid tumors.
authors.affiliation: Department of Surgical Oncology, Roswell Park Memorial Institute, Buffalo, New York 14263.
authors.forename: S
authors.initials: S
authors.lastname: Khan
authors.forename: A
authors.initials: A
authors.lastname: Raza
authors.forename: N
authors.initials: N
authors.lastname: Petrelli
authors.forename: A
authors.initials: A
authors.lastname: Mittleman
mesh_terms: D001973:Bromodeoxyuridine; D002277:Carcinoma / Q000473:pathology*; D002453:Cell Cycle; D003106:Colon / Q000166:cytology*; D015179:Colorectal Neoplasms / Q000473:pathology*; D004247:DNA / Q000032:analysis; D006801:Humans; D007262:Infusions, Intravenous; D007413:Intestinal Mucosa / Q000166:cytology; D009362:Neoplasm Metastasis
chemical_list: D004247:DNA; D001973:Bromodeoxyuridine