Entire nucleotide sequence of the pullulanase gene of Klebsiella aerogenes W70.
We determined the entire nucleotide sequence of the Klebsiella aerogenes W70 pullulanase gene (pulA) contained on a 4.2-kilobase-pair fragment of plasmid pPB174. The amino acid composition deduced from an open reading frame of 3,288 base pairs agreed closely with that determined for the intracellular pullalanase. The precursor enzyme consisted of 1,096 amino acid residues and contained a hydrophobic N-terminal signal peptide and the consensus sequence for the bacterial prelipoprotein signal peptide cleavage site.
Katsuragi
N
N
Takizawa
N
N
Murooka
Y
Y
D000595:Amino Acid Sequence; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D006026:Glycoside Hydrolases / Q000235:genetics*; D007711:Klebsiella pneumoniae / Q000235:genetics*
D004269:DNA, Bacterial; D006026:Glycoside Hydrolases; C100181:pullulanase