Adaptation of the rapid automated tetrazolium dye based (MTT) assay for chemosensitivity testing in childhood leukemia.
The reduction of the tetrazolium salt MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) to a blue-black formazan product by living but not by dead cells can be used to measure chemosensitivity of tumor cells. The main advantages of the MTT assay are its simplicity, rapidity, and the fact that the results are read automatically with a microplate spectrophotometer. Several reports on the use of the MTT assay in chemosensitivity testing have been published, but all these studies dealt with established cell lines and not with specimens obtained directly from patients. Here we present a study in which the MTT assay has been adapted to assess the effect of antineoplastic drugs on lymphoblasts of children with leukemia.
Pieters
R
R
Department of Pediatrics, Free University Hospital, Amsterdam, The Netherlands.
Huismans
D R
DR
Leyva
A
A
Veerman
A J
AJ
D000970:Antineoplastic Agents / Q000627:therapeutic use; D002470:Cell Survival / Q000187:drug effects; D002648:Child; D003114:Colony-Forming Units Assay / Q000379:methods*; D004121:Dimethyl Sulfoxide; D005562:Formazans / Q000032:analysis; D006801:Humans; D007945:Leukemia, Lymphoid / Q000188:drug therapy*; D013053:Spectrophotometry; D013778:Tetrazolium Salts; D013844:Thiazoles; D014407:Tumor Cells, Cultured / Q000187:drug effects; D014410:Tumor Stem Cell Assay / Q000379:methods*
D000970:Antineoplastic Agents; D005562:Formazans; D013778:Tetrazolium Salts; D013844:Thiazoles; C022616:thiazolyl blue; D004121:Dimethyl Sulfoxide