Cyclooxygenase products formed by primary cultures of cells from human chorion laeve: influence of steroids.
Cells were isolated from human chorion laeve obtained at term (38-40 weeks gestation) by elective caesarean section and were maintained in primary culture for 1 week in defined media supplemented with 10% fetal calf serum. The production of various cyclooxygenase products by the cultures was examined. Little or no prostaglandin (PG) F2 alpha, 6-keto-PGF1 alpha, thromboxane B2, or 13,14-dihydro-15-keto-PGF2 alpha was found. In contrast, the cells produced PGE2 which was low on day 0, increased during culture to a maximum on day 1 or 2, then declined to low levels. When cells were grown in the presence of media containing cortisol, dexamethasone, progesterone, and estradiol (at 10(-7) or 10(-9) M), the glucocorticoids (at 10(-7) and 10(-9) M), but not estrogen or progesterone, markedly inhibited the increase in PGE2 output. There was no difference in the protein content and thymidine incorporation of cells grown in the presence of glucocorticoids when compared with controls. This inhibitory effect was not sensitive to cycloheximide (1 microgram/mL) indicating protein synthesis may not be involved in the process. These studies indicate that PGE2 is the major prostaglandin formed by primary cultures of chorion laeve and that prostaglandin metabolism in the chorion is sensitive to glucocorticoid inhibition.
Gibb
W
W
Unit on Reproduction and Developmental Biology, Hôpital Ste-Justine, Montréal, Qué., Canada.
Riopel
L
L
Collu
R
R
Ducharme
J R
JR
Mitchell
M D
MD
Lavoie
J C
JC
D002478:Cells, Cultured; D002823:Chorion / Q000187:drug effects* / Q000378:metabolism; D003513:Cycloheximide / Q000494:pharmacology; D003907:Dexamethasone / Q000494:pharmacology; D005260:Female; D006801:Humans; D006854:Hydrocortisone / Q000494:pharmacology; D011247:Pregnancy; D011453:Prostaglandins / Q000096:biosynthesis*; D013256:Steroids / Q000494:pharmacology*; D013929:Thromboxane B2 / Q000096:biosynthesis*
D011453:Prostaglandins; D013256:Steroids; D013929:Thromboxane B2; D003907:Dexamethasone; D003513:Cycloheximide; D006854:Hydrocortisone