Synergism between U.V. and thymidine treatments in the induction of cytogenetic damage in wild-type Friend erythroleukaemia cells.
The cytogenetic aberration-inducing effect of excess thymidine and U.V. light in Friend erythroleukaemia (clone 707) cells was investigated. Three doses of U.V. were utilised, namely 2.4, 4.8 and 7.2 J/m2. Thymidine, at 1 X 10(-5) M, was present for 48 h prior to U.V. treatment and for 15 h following it. Although no significant increase in metaphase aberrations was observed following thymidine treatment alone, relative to the spontaneous frequency, clone 707 exhibited increased sensitivity to U.V.-induced cytogenetic damage, when grown in the presence of 1 X 10(-5) M thymidine. The observed synergism between U.V. and thymidine treatments may be due to thymidine-induced nucleotide pool imbalance with consequent inaccuracies in DNA repair.
McKelvey
V J
VJ
Biomedical Sciences Research Centre, University of Ulster, Coleraine, Northern Ireland.
McKenna
P G
PG
D000818:Animals; D002869:Chromosome Aberrations; D005622:Friend murine leukemia virus; D004915:Leukemia, Erythroblastic, Acute / Q000235:genetics* / Q000473:pathology; D007942:Leukemia, Experimental / Q000235:genetics* / Q000473:pathology; D051379:Mice; D013936:Thymidine / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000528:radiation effects; D014466:Ultraviolet Rays
D013936:Thymidine