The flow cytometry crossmatch in kidney transplantation.
1. Positive FCXM reactions were associated with sensitization in transplants with negative cytotoxic crossmatches. Recipients of regrafts, females, particularly those with previous pregnancies, and recipients with historical cytotoxic antibody reactivity were more likely to have FCXM detectable antibodies. 2. Primary nonfunction grafts were associated with a positive FCXM, but predominantly in those transplants involving female, older, or nontrauma donors. Nearly 40% of the FCXM positive transplants of these organs did not function during the first month posttransplant 3. Low 3-month graft survival rates were associated with a positive FCXM reaction, but once again this was seen with female, older, and nontrauma donor transplants. In addition, approximately 40% of FCXM positive transplants involving older or nontrauma donors that did survive had poor graft function at 3 months.
Cook
D J
DJ
UCLA Tissue Typing Laboratory, Department of Surgery, UCLA Center for Health Sciences.
Terasaki
P I
PI
Iwaki
Y
Y
Terashita
G
G
Takeda
A
A
Fujikawa
J
J
Lau
M
M
Danovitch
G
G
Rosenthal
J T
JT
Fine
R
R
D000961:Antilymphocyte Serum; D005434:Flow Cytometry; D006085:Graft Survival; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans; D007074:Immunoglobulin G; D007518:Isoantibodies; D016030:Kidney Transplantation / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology
D000961:Antilymphocyte Serum; D007074:Immunoglobulin G; D007518:Isoantibodies