title: Sensitive electrochemical high-performance liquid chromatography assay for the simultaneous determination of haloperidol and reduced haloperidol.
abstract: An electrochemical HPLC method for the simultaneous determination of haloperidol and reduced haloperidol in plasma is presented. Chlorohaloperidol serves as the internal standard. A cyanopropyl bond elut column was used for sample preparation. The eluate was evaporated and reconstituted with mobile phase and injected onto a nitrile bonded column. The chromatographic system consisted of an ESA Coulochem detector operated in the screen mode. Intra- and interassay coefficients of variation for both compounds were less than 7%, with a sensitivity limit of 20 pg on the column. A plasma level-time profile is presented to illustrate the sensitivity and applicability of this assay in small animal and human pharmacokinetic studies.
authors.affiliation: Department of Pharmaceutics, School of Pharmacy, University of Maryland, Baltimore 21201.
authors.forename: N D
authors.initials: ND
authors.lastname: Eddington
authors.forename: D
authors.initials: D
authors.lastname: Young
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D006168:Guinea Pigs; D006220:Haloperidol / Q000097:blood*; D006801:Humans; D008297:Male; D010084:Oxidation-Reduction
chemical_list: D006220:Haloperidol