Luminol-dependent chemiluminescence in antibody-sensitized neutrophils stimulated with protein A-bearing staphylococci.
When mouse polymorphonuclear leukocytes (PMNs) sensitized with rabbit antibody to mouse Ehrlich ascites tumor cells were stimulated by Staphylococcus aureus Cowan I cells, a conspicuous luminol-dependent chemiluminescence was observed in the absence of opsonin. The profile of the chemiluminescence (CL) response evoked by staphylococcal cells from antibody-sensitized PMNs had two peaks. An initial peak, observed within 1 min after stimulation, was sharp and high and a second peak, observed about 5 min after stimulation, was low and extended. The CL response of antibody-sensitized PMNs stimulated by S. aureus Cowan I cells was dose-dependently blocked by preincubation with soluble SpA. Cells of a mutant derived from S. aureus Cowan I strain with trace amounts of cell-bound SpA failed to stimulate the antibody-sensitized PMNs to generate the CL response. The antibody-sensitized PMNs were found to phagocytize SpA-bearing S. aureus cells even in the absence of opsonic serum. These results suggest that the observation presented here might provide a useful tool for the investigation of CL response of PMNs.
Nishihara
S
S
Department of Microbiology, Jikei University School of Medicine, Tokyo.
Seki
K
K
Ikigai
H
H
Masuda
S
S
D000818:Animals; D000906:Antibodies / Q000276:immunology; D008163:Luminescent Measurements; D008165:Luminol / Q000494:pharmacology*; D051379:Mice; D008813:Mice, Inbred ICR; D008854:Microscopy, Electron; D009504:Neutrophils / Q000276:immunology* / Q000378:metabolism / Q000648:ultrastructure; D010587:Phagocytosis; D011724:Pyridazines / Q000494:pharmacology*; D013205:Staphylococcal Protein A / Q000276:immunology*; D013211:Staphylococcus aureus / Q000276:immunology*
D000906:Antibodies; D011724:Pyridazines; D013205:Staphylococcal Protein A; D008165:Luminol