A convenient technique to compare the efficiency of promoters in Escherichia coli.
We describe a technique which allows one to insert any promoter in front of the chromosomal malPQ operon. This can be done easily by using only one plasmid, one strain, and two simple selections. Properties of the final chromosomal fusion are such that the level of amylomaltase, the product of the malQ gene, measures quantitatively the efficiency of the inserted promoter. This method was utilized to compare the efficiency of four well-known promoters: lacZp, trp, tac, lambdaPR and three malT activated promoters: malPp, malkP and malEp.
Vidal-Ingigliardi
D
D
Raibaud
O
O
D002876:Chromosomes, Bacterial; D004926:Escherichia coli / Q000235:genetics*; D005838:Genotype; D005964:Glucosyltransferases / Q000235:genetics; D006004:Glycogen Debranching Enzyme System; D009876:Operon; D010957:Plasmids; D013045:Species Specificity; D014158:Transcription, Genetic
D006004:Glycogen Debranching Enzyme System; D005964:Glucosyltransferases; C020569:4 alpha-glucanotransferase