Patterns of glycosidases in the histiocytic cell line U-937. Effects of agents inducing cell differentiation.
1. The cell bound glycosidases in sublines and clones of the histiocytic cell line U-937 have previously been shown to display characteristic patterns. 2. In this paper the effects of differentiation inducing agents upon glycosidase patterns of one subline, U-937 GTB, are presented. 3. Teleocidin, 12-O-tetradecanoyl-phorbol-13-acetate (TPA), dimethyl-sulfoxide (DMSO), dihydroxyvitamin D3 and supernatants from mixed lymphocyte culture (MCL) all induce cellular differentiation of U-937 GTB. 4. Significant changes of the levels of cell bound glycosidases were seen after addition of inducing agents. 5. Alterations have been monitored as relative effects upon the absolute glycosidase activities and as effects upon selected ratios of different glycosidases. 6. The separate inducing agents show distinct enzyme patterns.
Bjare
U
U
National Bacteriological Laboratory, Stockholm, Sweden.
Lundblad
G
G
Ivhed
I
I
Nilsson
K
K
D000135:Acid Phosphatase / Q000378:metabolism; D002117:Calcitriol / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D002999:Clone Cells / Q000201:enzymology; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004790:Enzyme Induction / Q000187:drug effects; D006026:Glycoside Hydrolases / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D016403:Lymphoma, Large B-Cell, Diffuse / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D008235:Lyngbya Toxins / Q000494:pharmacology; D010710:Phosphates / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D008235:Lyngbya Toxins; D010710:Phosphates; C022176:teleocidins; D000135:Acid Phosphatase; D006026:Glycoside Hydrolases; D002117:Calcitriol; D013755:Tetradecanoylphorbol Acetate; D004121:Dimethyl Sulfoxide