title: Activation of Ca2+-stimulated ATPase by phospholipid N-methylation in cardiac sarcoplasmic reticulum.
abstract: Incubation of cardiac sarcoplasmic reticulum (SR) in the presence of S-adenosyl-L-methionine, a methyl donor for the enzymatic N-methylation of phosphatidylethanolamine, increased Ca2+-stimulated ATPase activity. The increase in Ca2+-ATPase activity was not due to changes in the affinity for Ca2+ and was prevented by methyl acetimidate, an inhibitor of phospholipid N-methylation. The results suggest a possible regulatory role of phospholipid N-methylation in SR Ca2+-pump mechanism.
authors.forename: P K
authors.initials: PK
authors.lastname: Ganguly
authors.forename: V
authors.initials: V
authors.lastname: Panagia
authors.forename: K
authors.initials: K
authors.lastname: Okumura
authors.forename: N S
authors.initials: NS
authors.lastname: Dhalla
mesh_terms: D000818:Animals; D001693:Biological Transport, Active; D002118:Calcium / Q000378:metabolism; D000252:Calcium-Transporting ATPases / Q000378:metabolism*; D002474:Cell-Free System; D004789:Enzyme Activation; D007096:Imidoesters / Q000494:pharmacology; D008563:Membrane Lipids / Q000378:metabolism; D008745:Methylation; D009206:Myocardium / Q000201:enzymology*; D010713:Phosphatidylcholines / Q000378:metabolism*; D010714:Phosphatidylethanolamines / Q000378:metabolism*; D051381:Rats; D012436:S-Adenosylmethionine / Q000378:metabolism; D012519:Sarcoplasmic Reticulum / Q000201:enzymology*
chemical_list: D007096:Imidoesters; D008563:Membrane Lipids; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D012436:S-Adenosylmethionine; C010222:methyl acetimidate; D000252:Calcium-Transporting ATPases; D002118:Calcium