title: Selective vascular coagulation of rabbit colon using a flashlamp-excited dye laser operating at 577 nanometers.
abstract: Previous studies have demonstrated that brief pulses of selectively absorbed optical radiation can be used to confine thermal injury to pigmented targets within tissues. We performed studies in rabbits to assess the usefulness of this technique for selectively coagulating the colonic vasculature. By measuring the optical absorbance of rabbit colon with a spectrophotometer, it was determined that hemoglobin exhibits strong absorption relative to the rabbit colon at a wavelength of 577 nm. Because light must be absorbed to affect tissue, it was hypothesized that laser pulses of this wavelength would selectively damage blood vessels. This hypothesis was tested by examining the effect of 300-microseconds-long 577-nm laser pulses on rabbit colon in vivo. For delivered radiant exposures between 4 and 8 J/cm2, selective coagulation of the colonic vasculature could be produced without damage to the surrounding colon. At greater radiant exposures, vessel hemorrhage was occasionally noted but no transmural thermal injury was produced with delivered radiant exposures as high as 22 J/cm2. This technique may form the basis of a safe and simple treatment of vascular lesions of the colon such as angiodysplasia.
authors.affiliation: Department of Pathology, Massachusetts General Hospital, Boston.
authors.forename: N S
authors.initials: NS
authors.lastname: Nishioka
authors.forename: O T
authors.initials: OT
authors.lastname: Tan
authors.forename: B R
authors.initials: BR
authors.lastname: Bronstein
authors.forename: W A
authors.initials: WA
authors.lastname: Farinelli
authors.forename: J M
authors.initials: JM
authors.lastname: Richter
authors.forename: J A
authors.initials: JA
authors.lastname: Parrish
authors.forename: R R
authors.initials: RR
authors.lastname: Anderson
mesh_terms: D000818:Animals; D003106:Colon / Q000098:blood supply* / Q000473:pathology; D003108:Colonic Diseases / Q000209:etiology / Q000473:pathology; D003238:Connective Tissue / Q000098:blood supply / Q000473:pathology / Q000528:radiation effects; D004912:Erythrocytes / Q000528:radiation effects; D006470:Hemorrhage / Q000209:etiology / Q000473:pathology; D007413:Intestinal Mucosa / Q000098:blood supply / Q000473:pathology / Q000528:radiation effects*; D007834:Lasers; D008028:Light Coagulation / Q000009:adverse effects / Q000379:methods; D011817:Rabbits