Further characterization of SOS system induction in recBC mutants of Escherichia coli.
Expression of several SOS functions such as induction of lambda prophage, inhibition of cell division and induction of both umuC and recA genes after UV-irradiation, nalidixic acid or mitomycin C addition was studied in an RecBC- mutant. UV-irradiation and mitomycin C induced all SOS functions studied in the RecBC- cells but at a lower level and delayed with respect to the wild-type strain. On the contrary, nalidixic acid was unable to trigger any of these SOS functions. In the RecBC- mutant, adenine only had a stimulating effect on the amplification of RecA protein synthesis following UV-irradiation. Nevertheless, in the wild-type strain the stimulating effect occurred in all SOS functions studied following UV-irradiation as well as in the amplification of RecA protein synthesis by nalidixic acid but not in the other SOS functions triggered by this compound. Furthermore, adenine produced a decrease in the mitomycin C-mediated induction of all SOS functions studied in both RecBC- and wild-type strains.
Barbé
J
J
Vericat
J A
JA
Cairó
J
J
Guerrero
R
R
D000225:Adenine / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000378:metabolism; D010582:Bacteriophage lambda / Q000187:drug effects; D004260:DNA Repair; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D005798:Genes, Bacterial; D009154:Mutation; D009268:Nalidixic Acid / Q000494:pharmacology; D014775:Virus Activation / Q000187:drug effects
D009268:Nalidixic Acid; D000255:Adenosine Triphosphate; D000225:Adenine