Syngeneic responses by murine thymocytes: a role for non-MHC and non-MLS genes.
A subpopulation of thymocytes from adult mice that is nonadherent to macrophage monolayers showed dramatically increased syngeneic mixed leukocyte responses (SMLR). Cloned cells were derived by limiting dilution from these SMLR-primed BALB/c thymocytes and were maintained and subcloned by repeated stimulation with syngeneic BALB/c spleen cells without the addition of exogenous interleukins. The cloned thymocytes were tested for their reactivities against H-2- and Mls-identical BALB/c and B10.D2 spleen cells (H-2d, Mlsb). We found that BALB/c and B10.D2 stimulator cells differed significantly in their capacity to restimulate the cloned BALB/c thymocytes. In addition, polyclonal syngeneic mixed leukocyte cultures (SMLC) of BALB/c thymocytes also showed differential restimulation by BALB/c and B10.D2 stimulators. Taken together, our data indicate a role for the product(s) specified by non-MHC and non-Mls gene(s) in the autorecognition by thymocytes.
Wu
S
S
Thomas
D W
DW
D000818:Animals; D002478:Cells, Cultured; D002999:Clone Cells; D005802:Genes, MHC Class II; D007376:Interleukin-2 / Q000032:analysis; D007959:Lymphocyte Culture Test, Mixed; D017710:Lymphocyte Transfusion; D008214:Lymphocytes / Q000276:immunology; D008264:Macrophages / Q000276:immunology; D008285:Major Histocompatibility Complex; D051379:Mice; D008807:Mice, Inbred BALB C; D008811:Mice, Inbred DBA; D008815:Mice, Inbred Strains; D013045:Species Specificity; D013154:Spleen / Q000276:immunology; D013950:Thymus Gland / Q000276:immunology; D014185:Transplantation, Isogeneic
D007376:Interleukin-2