Evaluation of a radioimmunoassay for histamine measurement in biologic fluids.
A new radioimmunoassay for the measurement of histamine in biologic fluids was evaluated. Assay selectivity and specificity were achieved by "succinyl-glycinamide derivatization" of histamine in samples to mimic the immunogen used to generate the monoclonal antibody. The assay exhibits a linear response from 0.1 to 5.0 ng/ml of histamine and the monoclonal antibody used has partial recognition of only N-methylhistamine (other than histamine). With minimal modifications, the assay can accurately measure histamine in plasma, urine, and buffer. Normal ranges for human subjects were established: plasma levels are 0.193 +/- 0.08 ng/ml (n = 40) and urine levels are 20.9 +/- 11.2 micrograms histamine/gm creatinine (n = 10).
McBride
P
P
Allergic Diseases Section, National Institutes of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Md.
Bradley
D
D
Kaliner
M
M
D001826:Body Fluids / Q000032:analysis*; D002855:Chromatography, Thin Layer; D005260:Female; D006632:Histamine / Q000032:analysis*; D006801:Humans; D006967:Hypersensitivity / Q000503:physiopathology; D008297:Male; D011863:Radioimmunoassay / Q000379:methods*
D006632:Histamine