Further evidence that bovine IgM does not fix guinea pig complement.
In a study of sera from cattle vaccinated with 3 X 10(10) cfu of Brucella abortus strain 19, it was found that IgG1 antibody measured by an indirect ELISA was the only isotype to correlate with standard complement fixing antibody titers using heated serum samples and guinea pig serum as a source of complement. A supplement of normal unheated bovine serum resulted in IgM fixing guinea pig complement, giving data similar to those obtained with unheated serum in the complement fixation test.
Nielsen
K
K
Agriculture Canada, Animal Diseases Research Institute, Nepean, Ontario.
Duncan
J R
JR
D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D002003:Brucella abortus / Q000276:immunology; D002007:Brucellosis, Bovine / Q000276:immunology / Q000517:prevention & control; D002417:Cattle / Q000276:immunology*; D003167:Complement Activation; D003168:Complement Fixation Tests; D006168:Guinea Pigs / Q000276:immunology*; D007074:Immunoglobulin G / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology*; D066298:In Vitro Techniques; D013045:Species Specificity
D000907:Antibodies, Bacterial; D007074:Immunoglobulin G; D007075:Immunoglobulin M