In vivo measurement of intracellular pH in human brain during different tensions of carbon dioxide in arterial blood. A 31P-NMR study.
The effect of changes in carbon dioxide tension in arterial blood upon intracellular pH in brain tissue was studied in seven healthy volunteers, aged 22-45 years. The pH changes were monitored by use of 31P nuclear magnetic resonance spectroscopy, performed on a whole-body 1.5 Tesla Siemens imaging system. The measurements were carried out during hyperventilation and with the subject breathing atmospheric air containing 5 vol. % and 7 vol. % carbon dioxide. Intracellular pH increased significantly during 15 min of hyper-ventilation and decreased significantly during 18 min respiration of air containing 7 vol. % carbon dioxide. The intracellular buffer capacity was estimated. These results suggest that the ventilation response to carbon dioxide is correlated to changes in intracellular fluid pH.
Jensen
K E
KE
Department of Magnetic Resonance, Hvidovre Hospital, University of Copenhagen, Denmark.
Thomsen
C
C
Henriksen
O
O
D000328:Adult; D001826:Body Fluids / Q000502:physiology*; D001921:Brain / Q000502:physiology*; D002245:Carbon Dioxide / Q000097:blood*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007424:Intracellular Fluid / Q000502:physiology*; D009682:Magnetic Resonance Spectroscopy; D008875:Middle Aged; D010313:Partial Pressure
D002245:Carbon Dioxide