Circadian variations of autologous mixed lymphocyte reactions and endogenous cortisol.
The degree of proliferation of human T cells stimulated with autologous PHA-T cells and with autologous non-T cells displays circadian variations. The highest proliferation occurs with cells isolated from blood drawn at 8 a.m. in mixed lymphocyte reactions (MLR) with autologous PHA-T cells and from blood drawn at 8 p.m. in MLR with autologous non-T cells. The circadian variations of autologous MLRs appear to reflect changes in the proliferative response of T cells. In autologous MLRs with non-T cells as stimulators the extent of proliferation was inversely correlated with the level of endogenous cortisol. The circadian variations of autologous MLRs do not reflect non-specific changes in the proliferative and stimulatory properties of T and non-T cells, since circadian variations were not observed in the proliferative response of T cells to mitogens and in allogeneic MLRs. Circadian variations of autologous MLRs must be taken into account when analyzing abnormalities of these reactions in pathological conditions.
Indiveri
F
F
Pierri
I
I
Rogna
S
S
Poggi
A
A
Montaldo
P
P
Romano
R
R
Pende
A
A
Morgano
A
A
Barabino
A
A
Ferrone
S
S
D002940:Circadian Rhythm; D006801:Humans; D006854:Hydrocortisone / Q000097:blood*; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D008214:Lymphocytes / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology
D006854:Hydrocortisone