Metabolism of glutamate and ammonia in astrocyte: an immunocytochemical study.
Alpha-ketoglutarate (alpha-KG) reductive amination activity in rat brain was found to be mostly absorbed with an antibody against liver glutamate dehydrogenase. With this and anti-glutamine synthetase antibodies, alpha-KG reductive amination activity was immunocytochemically shown to coexist with glutamine synthetase activity in astrocytes. The results suggest that astrocytes de novo synthesize glutamate from alpha-KG and ammonia, and metabolize it to glutamine.
Kaneko
T
T
Department of Anatomy (1st Division), Faculty of Medicine, Kyoto University, Japan.
Shigemoto
R
R
Mizuno
N
N
D000586:Amination; D000818:Animals; D001253:Astrocytes / Q000201:enzymology*; D001921:Brain / Q000201:enzymology; D005969:Glutamate Dehydrogenase / Q000276:immunology / Q000378:metabolism*; D005972:Glutaminase / Q000276:immunology / Q000378:metabolism*; D007150:Immunohistochemistry; D007656:Ketoglutaric Acids / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D007656:Ketoglutaric Acids; D005969:Glutamate Dehydrogenase; D005972:Glutaminase