title: Differential structure-activity relationships of atrial peptides as natriuretics and renal vasodilators in the dog.
abstract: Natriuretic-diuretic and vasodilator activities of synthetic atriopeptin (AP)-related peptides were examined in the anesthetized dog. We have selected, the naturally occurring, APIII as the reference compound for comparison with various related peptides. APIII is a 24 amino acid peptide with the sequence ser-ser-cys-phe-gly-gly-arg-ile-asp-arg-ile-gly-ala-gln-ser-gly-leu-gly- cys-asn-ser-phe-arg-tyr-OH. APII, another peptide isolated from atrial extracts, lacks the C-terminal arg- of APIII. N-terminal amino acid extensions on APIII or APII, exhibited enhanced natriuretic-diuretic effectiveness. Furthermore, the maximum response obtained by ser-leu-arg-arg-APIII and arg-arg-APIII were significantly higher and the dose-response curve was not parallel to that obtained with APIII. In contrast, there were no significant qualitative or quantitative differences between the renal blood flow responses produced by the N-terminal extended peptides and APII or APIII. These results suggest a heterogeneity of AP receptors in vascular and renal tubular tissues.
authors.forename: N
authors.initials: N
authors.lastname: Katsube
authors.forename: K
authors.initials: K
authors.lastname: Wakitani
authors.forename: K F
authors.initials: KF
authors.lastname: Fok
authors.forename: F S
authors.initials: FS
authors.lastname: Tjoeng
authors.forename: M E
authors.initials: ME
authors.lastname: Zupec
authors.forename: S R
authors.initials: SR
authors.lastname: Eubanks
authors.forename: S P
authors.initials: SP
authors.lastname: Adams
authors.forename: P
authors.initials: P
authors.lastname: Needleman
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D009320:Atrial Natriuretic Factor; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D007668:Kidney / Q000098:blood supply*; D008297:Male; D009124:Muscle Proteins / Q000494:pharmacology*; D009318:Natriuresis / Q000187:drug effects*; D013329:Structure-Activity Relationship; D014664:Vasodilation / Q000187:drug effects*
chemical_list: D009124:Muscle Proteins; D009320:Atrial Natriuretic Factor