Steric protection of a modified glass surface with adsorbed polyacrylamide: interactions with blood proteins.
Glass beads chemically modified with aluminol (A1OH) groups were coated with a monolayer of high-molecular-mass polyacrylamide at different temperatures and exposed to 125I-labelled albumin and fibrinogen solutions for 1 h. No adsorption of albumin was observed, whereas fibrinogen adsorption increased as a function of temperature. These observations were interpreted in terms of the density and stability of the adsorbed polymer layer. At 25 degrees C, where fibrinogen adsorption was already important, it was shown, using 3H-labelled polyacrylamide, that fibrinogen partly displaced the polymer from its adsorption sites.
Voegel
J C
JC
Institut Charles Sadron (CRM-EAHP), CNRS-ULP Strasbourg, France.
Pefferkorn
E
E
Schmitt
A
A
D000180:Acrylic Resins; D000327:Adsorption; D001798:Blood Proteins / Q000032:analysis*; D002021:Buffers; D055598:Chemical Phenomena; D002621:Chemistry; D005340:Fibrinogen / Q000032:analysis; D005898:Glass; D006801:Humans; D013499:Surface Properties; D013696:Temperature; D000099166:Polyacrylamides
D000180:Acrylic Resins; D001798:Blood Proteins; D002021:Buffers; D005340:Fibrinogen; D000099166:Polyacrylamides