Identification of a biochemical marker for the secretory pathway in Tetrahymena thermophila.
Tetrahymena thermophila is a ciliated protozoan studied by investigators from a wide range of disciplines as a model system for a variety of specialized eukaryotic cell functions. The proteinaceous secretory products of T thermophila have been isolated and characterized and in this study we identify the major secretory product, a 34,000 Mr polypeptide, and use an antiserum prepared against this secretory protein to (1) demonstrate that this 34,000 Mr polypeptide is truly a secreted protein in Tetrahymena and (2) monitor the synthesis and transport of this protein by indirect immunofluorescence and light microscopy during mucocyst biogenesis.
Maihle
N J
NJ
Albert Einstein College of Medicine, Department of Anatomy and Structural Biology, Bronx, New York 10461.
Satir
B H
BH
D000818:Animals; D000953:Antigens, Protozoan / Q000502:physiology*; D007158:Immunologic Techniques; D008970:Molecular Weight; D015800:Protozoan Proteins / Q000378:metabolism*; D013768:Tetrahymena / Q000276:immunology / Q000378:metabolism*
D000953:Antigens, Protozoan; D015800:Protozoan Proteins