Analysis of DNA from cells fixed in Carnoy's solution for cytogenetic study.
DNA for Southern blot analysis was obtained from cells fixed in Carnoy's solution for cytogenetic investigation. DNA samples were extracted from fixed cells obtained from Philadelphia chromosome positive chronic myelogenous leukemia patients and shown to have aberrant fragments hybridized with a bcr probe; no apparent degradation was noted. This method should be useful for detection of molecular changes in cells fixed for chromosome analysis.
Ohyashiki
J H
JH
First Department of Internal Medicine, Tokyo Medical College, Japan.
Ohyashiki
K
K
Toyama
K
K
D000085:Acetates; D019342:Acetic Acid; D002725:Chloroform; D002869:Chromosome Aberrations; D004273:DNA, Neoplasm / Q000032:analysis*; D000431:Ethanol; D005404:Fixatives; D006801:Humans; D007951:Leukemia, Myeloid / Q000235:genetics* / Q000473:pathology
D000085:Acetates; C033474:Carnoy's solution; D004273:DNA, Neoplasm; D005404:Fixatives; D000431:Ethanol; D002725:Chloroform; D019342:Acetic Acid