title: Glycosaminoglycan containing fat-storing cells in hepatic fibrogenesis.
abstract: Male F-344 rats were treated for 10 weeks either with CCl4 (0.2 ml/kg, per os, twice a week) or with CCl4 (same as above) and phenobarbital (0.2 g/l in drinking water). Liver fibrosis and cirrhosis developed in both treated groups, and was confirmed histologically. Cirrhosis was more frequent after the CCl4 + phenobarbital treatment. The collagen content of the liver, measured by morphometry and biochemically, was significantly higher in the animals of the group treated with CCl4 + phenobarbital than in the animals treated only with CCl4. Specially altered fat-storing cells (Ito cells) were found in the periportal and septal fibrotic areas in direct proportion to the amount of fibrosis and cirrhosis. They were identified as altered fat-storing cells by their desmin content and Vitamin A storing capability. This study demonstrated that these cells were enlarged and contained neutral fat, lipofuscin and PAS-positive material. The potential role of GAG-containing FSC in fibrogenesis is discussed.
authors.affiliation: 1st Institute of Pathology and Experimental Cancer Research, Semmelweis Medical School, Budapest, Hungary.
authors.forename: B
authors.initials: B
authors.lastname: Szende
authors.forename: K
authors.initials: K
authors.lastname: Lapis
authors.forename: J
authors.initials: J
authors.lastname: Timár
authors.forename: Z
authors.initials: Z
authors.lastname: Schaff
authors.forename: K
authors.initials: K
authors.lastname: Simon
authors.forename: A
authors.initials: A
authors.lastname: Jeney
authors.forename: A
authors.initials: A
authors.lastname: Divald
authors.forename: S
authors.initials: S
authors.lastname: Paku
authors.forename: F
authors.initials: F
authors.lastname: Timár
mesh_terms: D000818:Animals; D002251:Carbon Tetrachloride; D006025:Glycosaminoglycans / Q000032:analysis* / Q000378:metabolism; D050356:Lipid Metabolism; D008055:Lipids / Q000032:analysis*; D008099:Liver / Q000473:pathology* / Q000648:ultrastructure; D008103:Liver Cirrhosis / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology*; D008297:Male; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D010634:Phenobarbital; D051381:Rats; D011916:Rats, Inbred F344
chemical_list: D006025:Glycosaminoglycans; D008055:Lipids; D002251:Carbon Tetrachloride; D010634:Phenobarbital