Purification of a low molecular weight factor that induces differentiation and inhibits growth in myeloid leukemia cells.
A procedure is described for purifying a low molecular weight peptide that induces differentiation in mouse myeloid leukemia M1 cells. The factor comes from the conditioned medium of macrophage-like cells differentiated from mouse myeloid leukemia M1 cells. The procedure for purification includes gel filtration on Sephadex G-15, anionic exchange chromatography, thin-layer chromatography, reverse-phase high-performance liquid chromatography on a C18 hydrophobic support, and high-performance liquid chromatography gel filtration. The molecular weight of the factor estimated from the amino acid composition was approximately 1280, which agrees well with that obtained by high-performance liquid chromatography gel filtration. The half-maximal concentration of the purified factor for inducing differentiation of M1 cells was approximately 3.2 x 10(-7) M as judged by nitroblue tetrazolium staining ability. The purified factor also inhibits the growth of human leukemia HL-60 cells.
Nakaya
K
K
School of Pharmaceutical Sciences, Showa University, Tokyo, Japan.
Kumakawa
N
N
Iinuma
H
H
Nakamura
Y
Y
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D002855:Chromatography, Thin Layer; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000473:pathology*; D051379:Mice; D008970:Molecular Weight; D009580:Nitroblue Tetrazolium; D010455:Peptides / Q000032:analysis / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D010455:Peptides; D009580:Nitroblue Tetrazolium