Sensitivity to alpha/beta-interferon is independent of N-linked complex-type oligosaccharides on cell-surface-membrane glycoproteins.
The extent of involvement of carbohydrate structures in the mechanism of action of alpha and beta-interferon (IFN-alpha, IFN-beta) is undefined. In this report we examine the role of complex-type N-linked oligosaccharides in the response to these interferons. The response of mouse leukemia L 1210S cells, grown in the presence of swainsonine, an inhibitor of Golgi mannosidase II [Tulsiani, D. R. P., Harris, T. M. and Touster, O. (1982) J. Biol. Chem. 257, 7936-7939; Elbein A. D., Solf, R., Dorling, P. R. and Vosbeck, K. (1981) Proc. Natl Acad. Sci. USA 78, 7393-7397], to mouse IFN-alpha/beta, both with respect to antiviral and antigrowth effects, remains intact in spite of the total absence of complex-type N-linked oligosaccharides. Also, there is no difference in the response to human IFN-beta of a parental Chinese hamster ovary cell line and a mutant lacking beta-N-acetylglucosaminyltransferase I and therefore unable to synthesize complex-type N-linked oligosaccharides [Stanley, P., Callibot, V. and Siminovitch, L. (1975) Cell 6, 121-128]. These results are significant in permitting the conclusion that the carbohydrate-specific binding of IFN-alpha and IFN-beta to gangliosides cannot be due to a similarity of the ganglioside carbohydrate to that of a glycoprotein containing a complex-type N-liked oligosaccharide.
Parker
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J
Ankel
H
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D000470:Alkaloids / Q000494:pharmacology; D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D005260:Female; D005964:Glucosyltransferases / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism*; D007370:Interferon Type I / Q000378:metabolism / Q000494:pharmacology*; D037102:Lectins / Q000494:pharmacology; D007939:Leukemia L1210 / Q000378:metabolism; D008361:Mannosidases / Q000037:antagonists & inhibitors; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D009154:Mutation; D017351:N-Acetylglucosaminyltransferases; D009844:Oligosaccharides / Q000378:metabolism*; D010053:Ovary; D011971:Receptors, Immunologic / Q000378:metabolism*; D017471:Receptors, Interferon; D017026:Swainsonine
D000470:Alkaloids; D006023:Glycoproteins; D007370:Interferon Type I; D037102:Lectins; D008565:Membrane Proteins; D009844:Oligosaccharides; D011971:Receptors, Immunologic; D017471:Receptors, Interferon; D005964:Glucosyltransferases; D017351:N-Acetylglucosaminyltransferases; C037649:N-acetyllactosaminide beta-1,6-N-acetylglucosaminyltransferase; D008361:Mannosidases; C038829:mannosyl-oligosaccharide 1,3 - 1,6-alpha-mannosidase; D017026:Swainsonine