Serotonin and melatonin synthesis in peripheral blood mononuclear cells: stimulation by interferon-gamma as part of an immunomodulatory pathway.
Serotonin and melatonin inhibit phytohemagglutinin- (PHA) induced interferon-gamma (IFN-gamma) production by lymphocytes. In this paper, it is shown that IFN-gamma-increased tryptophan uptake by lymphocytes and macrophages led to an enhanced production of serotonin. When IFN-gamma and serotonin were added together to a lymphocyte culture, N-acetyl serotonin and melatonin production was increased, whereas the path to 5-hydroxy-indoleacetic acid remained unchanged. Therefore, the stimulated IFN-gamma production of serotonin and melatonin by lymphocytes and macrophages and the inhibition of IFN-gamma synthesis by these indoleamines suggest a hypothesis for an immunoregulatory circuit.
Finocchiaro
L M
LM
Consejo Nacional de Investigaciones Cientificas y Technicas (C.O.N.I.C.E.T.), República Argentina.
Arzt
E S
ES
Fernández-Castelo
S
S
Criscuolo
M
M
Finkielman
S
S
Nahmod
V E
VE
D002478:Cells, Cultured; D007371:Interferon-gamma / Q000494:pharmacology*; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000378:metabolism*; D008550:Melatonin / Q000096:biosynthesis*; D010835:Phytohemagglutinins / Q000494:pharmacology; D012701:Serotonin / Q000096:biosynthesis* / Q000378:metabolism; D013601:T-Lymphocytes / Q000187:drug effects / Q000378:metabolism; D014364:Tryptophan / Q000378:metabolism
D010835:Phytohemagglutinins; D012701:Serotonin; D007371:Interferon-gamma; D014364:Tryptophan; D008550:Melatonin