Two-dimensional NMR analyses of dynamic structures of tRNA and the regulation of codon recognition by post-transcriptional modifications.
By two-dimensional nuclear magnetic resonance (2D-NMR) spectroscopy, we analyzed dynamic structures of various tRNA species from Escherichia coli and Bacillus subtilis. Proton resonances due to the anticodon of the tRNA molecules were unambiguously identified by NOESY and 2D-HOHAHA techniques. Thus, it was found that rigidity/flexibility of the two types of modified uridines in the first position of the anticodon were certainly related with the codon recognition properties of the tRNA species.
Kawai
G
G
Department of Biophysics and Biochemistry, Faculty of Science, University of Tokyo, Japan.
Yokoyama
S
S
Hasegawa
T
T
Murao
K
K
Ishikura
H
H
Nishimura
S
S
Miyazawa
T
T
D000926:Anticodon; D001412:Bacillus subtilis / Q000235:genetics; D003062:Codon; D004926:Escherichia coli / Q000235:genetics; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D012323:RNA Processing, Post-Transcriptional; D012343:RNA, Transfer / Q000235:genetics / Q000648:ultrastructure*
D000926:Anticodon; D003062:Codon; D012343:RNA, Transfer