Comparison of a medium supplement--'Nu-Serum'--and foetal calf serum in RPMI 1640 used for the murine allogeneic mixed lymphocyte reaction.
A commercially available medium supplement, Nu-Serum, was compared with foetal calf serum for its ability to support the murine allogeneic mixed lymphocyte reaction. Nu-Serum supported proliferation whilst at the same time producing significantly lower counts in unstimulated cultures. There was considerably less batch-to-batch variation with Nu-Serum than with foetal calf serum, which obviated the need for pretesting in order to find a suitable batch.
Gibb
I A
IA
Webber
D G
DG
Bowen
J G
JG
D000367:Age Factors; D000818:Animals; D002417:Cattle; D003470:Culture Media; D005260:Female; D066298:In Vitro Techniques; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D051379:Mice; D008815:Mice, Inbred Strains
D003470:Culture Media