Radioimmunoassay of atrial natriuretic factor: human plasma levels.
A simple and sensitive radioimmunoassay procedure has been developed for the determination of atrial natriuretic factor (ANF) in human plasma. The rabbit antiserum was obtained from a commercial source. ANF was extracted from plasma using an octadecasilyl silica cartridge with a recovery of 78.7%. HPLC of the plasma extract showed the presence of one immunoreactive peak of ANF corresponding to its low molecular weight form. Plasma ANF in humans increased from 8.0 +/- 2.2 in upright position to 20.0 +/- 5.9 fmol/ml (n = 6) in downward position (p less than 0.005).
Larose
P
P
Meloche
S
S
du Souich
P
P
Deléan
A
A
Ong
H
H
D000818:Animals; D009320:Atrial Natriuretic Factor; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D009124:Muscle Proteins / Q000097:blood*; D011817:Rabbits; D011863:Radioimmunoassay / Q000379:methods; D051381:Rats
D009124:Muscle Proteins; C472955:NPPA protein, human; D009320:Atrial Natriuretic Factor