Delay in onset of insulitis in NOD mice following a single injection of CD 4 and CD 8 antibodies.
Non-obese diabetic (NOD) mice injected with 500 micrograms of both CD 4 and CD 8 antibodies at 5 weeks of age did not develop insulitis until 18 weeks of age, 12 weeks later than the onset of insulitis in parallel uninjected controls. Injection with both antibodies at 2 weeks or 4 weeks of age protected from insulitis for 10 and 14 weeks respectively. Insulitis was not delayed in onset in animals injected at any age with one antibody only, or who were injected at birth. Injection after the onset of insulitis achieved partial but incomplete clearing of islet infiltrates. Salivary gland infiltrates (sialitis) were also delayed in animals injected with both CD 4 and CD 8 antibodies though the degree of protection was less pronounced than that seen for insulitis.
Hayward
A R
AR
Division of Transplantation Biology, Northwick Park Hospital, London, U.K.
Cobbold
S P
SP
Waldmann
H
H
Cooke
A
A
Simpson
E
E
D000367:Age Factors; D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte / Q000627:therapeutic use*; D001327:Autoimmune Diseases / Q000517:prevention & control*; D016827:CD8 Antigens; D002469:Cell Separation; D005434:Flow Cytometry; D007267:Injections; D007515:Islets of Langerhans / Q000276:immunology*; D051379:Mice; D012469:Salivary Glands / Q000276:immunology; D013997:Time Factors
D000945:Antigens, Differentiation, T-Lymphocyte; D016827:CD8 Antigens