Determination of betamethasone 17-benzoate in lipophylic vehicles by reversed-phase high-performance liquid chromatography.
A stability-indicating, reversed-phase HPLC method was developed to assay betamethasone 17-benzoate in lipophylic bases. An on-line precolumn sample clean-up system was used to protect the analytical column from contamination; it consisted of the Waters Guard-Pak Precolumn Module and the muBondapak C18 Precolumn Insert. The method is simple, specific, accurate, and precise. It is also cost effective because it extends the life of the HPLC column.
Maron
N
N
Warner-Lambert Canada, Inc., Research and Development, Scarborough, Ontario, Canada.
Cristi
E A
EA
Ramos
A A
AA
D001623:Betamethasone / Q000031:analogs & derivatives* / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D007202:Indicators and Reagents; D009824:Ointments; D014677:Pharmaceutical Vehicles; D013488:Suppositories
D007202:Indicators and Reagents; D009824:Ointments; D014677:Pharmaceutical Vehicles; D013488:Suppositories; C015706:betamethasone benzoate; D001623:Betamethasone