title: Proliferative heterogeneity of vascular smooth muscle cells and its alteration by injury.
abstract: This study was designed to explore the question of whether the population of morphologically similar smooth muscle cells (SMC) in the vessel wall is functionally homogeneous or heterogeneous with respect to their proliferative response to injury. Using time-lapse video recording we measured interdivision times (IDT) of primary SMC clones, sibling pairs, and mother/daughter pairs. SMC from in vivo undisturbed vessels displayed an interclonal and intraclonal heterogeneity with a wide range in IDT. In vivo balloon injury resulted in a population with homogeneously short IDT. While 80% of IDT of SMC from injured vessels were shorter than 14 h, only slightly more than half of IDT of cells from undisturbed vessels fell into this category. Longitudinal analysis of mother/daughter pairs confirmed the presence of a heterogeneous population of SMC in the undisturbed vessel wall. In vivo balloon injury not only shortened the IDT of the majority of cells, but the shorter IDT persisted much longer than in the case of the undisturbed vessel. We suggest that a morphologically homogeneous SMC population in the aorta can now be subdivided into several groups of functionally different SMC with respect to their proliferative response to injury.
authors.forename: C C
authors.initials: CC
authors.lastname: Haudenschild
authors.forename: J
authors.initials: J
authors.lastname: Grunwald
mesh_terms: D000800:Angioplasty, Balloon; D000818:Animals; D001011:Aorta / Q000293:injuries; D002999:Clone Cells; D046508:Culture Techniques; D007700:Kinetics; D008297:Male; D008938:Mitosis; D009131:Muscle, Smooth, Vascular / Q000166:cytology*; D051381:Rats; D011919:Rats, Inbred Strains