Separation of Crassostrea gigas hemocytes by density gradient centrifugation and counterflow centrifugal elutriation.
A protocol is described to separate several subpopulations of hemocytes in a unique medium which avoids cell aggregation and retains cell-viability. Isopycnic centrifugation in Percoll followed by counterflow centrifugal elutriation provides large quantities of separated granulocyte and hyalinocyte subpopulations.
Bachère
E
E
IFREMER, Laboratoire de pathologie et de Génétique des Invertébrés Marins, La Tremblade, France.
Chagot
D
D
Grizel
H
H
D000818:Animals; D001773:Blood Cells / Q000166:cytology*; D002469:Cell Separation / Q000379:methods*; D002498:Centrifugation / Q000379:methods; D002500:Centrifugation, Isopycnic; D006434:Hemocytes / Q000166:cytology* / Q000648:ultrastructure; D008854:Microscopy, Electron; D010125:Ostreidae / Q000166:cytology*