title: A study on the quaternary structure change of hemoglobin in the ligation process.
abstract: In order to inquire into the molecular mechanism underlying the cooperative ligand binding to hemoglobin (Hb), conformational interaction at the interfaces between subunits are investigated on the basis of the atomic coordinates of human deoxy and human carbonmonoxy Hbs. Hypothetical intermediate structures are used, each of which is obtained from the procedure where one or more subunits in deoxy Hb are replaced by the corresponding CO-liganded subunits in carbonmonoxy Hb using the method of superimposition of two sets of atomic coordinates. When either alpha or beta subunit is substituted with the corresponding subunit in carbonmonoxy Hb, serious steric hindrances are produced between alpha 1FG4(92)Arg and beta 2C3(37)Trp or between alpha 1C6(41)Thr and beta 2FG4(97)His, all of which belong to the allosteric core affected directly by ligand binding. These steric hindrances become more serious when both alpha 1(alpha 2) and beta 2(beta 1) subunits are substituted. Therefore the change in the relative distance between iron atom and porphyrin by ligation results in strain in the C-terminal residues as an effect of the steric hindrance between the FG and C segments. However, no steric hindrance can be seen between subunits when the subunits in carbonmonoxy Hb are substituted with the corresponding subunits in deoxy Hb. The nature of the quaternary structural change from liganded to deoxy Hb seems to be different from that from deoxy to liganded Hb.
authors.affiliation: Department of Technical Education, Faculty of Education, University of the Ryukyus, Okinawa, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Arata
authors.forename: Y
authors.initials: Y
authors.lastname: Seno
authors.forename: J
authors.initials: J
authors.lastname: Otsuka
mesh_terms: D002151:Calorimetry; D002263:Carboxyhemoglobin / Q000378:metabolism*; D006454:Hemoglobins / Q000378:metabolism*; D006801:Humans; D046911:Macromolecular Substances; D008433:Mathematics; D008958:Models, Molecular; D008962:Models, Theoretical; D011487:Protein Conformation
chemical_list: D006454:Hemoglobins; D046911:Macromolecular Substances; D002263:Carboxyhemoglobin