A new trisaccharide sugar chain linked to a serine residue in bovine blood coagulation factors VII and IX.
A new trisaccharide sugar chain was identified in bovine blood coagulation factors VII and IX. A pentapeptide isolated from factor VII contained Ser-52, which could not be identified with a gas-phase sequencer, suggesting an unknown substituent on the serine residue (Takeya, H. et al. (1988) J. Biol. Chem., in press). The same results were obtained for a pentapeptide containing Ser-53 of factor IX. Component sugar analysis revealed that the peptide contained 1 mol of glucose and 2 mol of xylose. This sugar component was also confirmed by high-resolution fast atom bombardment mass spectrometric analysis of the pentapeptide. The trisaccharide was released from the peptides by means of beta-elimination reaction and its reducing end was coupled with 2-aminopyridine. The fluorescent pyridylamino (PA-) derivative of the trisaccharide was purified by gel-filtration and reversed-phase HPLC. The sugar composition of the PA-trisaccharide was found to be 2 mol of xylose and 1 mol of PA-glucose. These results indicate the existence of a (Xyl2)Glc-Ser structure in factors VII and IX.
Hase
S
S
Department of Chemistry, Osaka University College of Science.
Kawabata
S
S
Nishimura
H
H
Takeya
H
H
Sueyoshi
T
T
Miyata
T
T
Iwanaga
S
S
Takao
T
T
Shimonishi
Y
Y
Ikenaka
T
T
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002240:Carbohydrate Sequence; D002417:Cattle; D005164:Factor IX / Q000032:analysis*; D005167:Factor VII / Q000032:analysis*; D008969:Molecular Sequence Data; D012694:Serine; D014312:Trisaccharides / Q000032:analysis
D000596:Amino Acids; D014312:Trisaccharides; D012694:Serine; D005167:Factor VII; D005164:Factor IX