In vitro blastogenesis of porcine peripheral blood lymphocytes.
A microculture technique was used for the in vitro blastogenesis of pig lymphocytes. The effects of some variable on the stimulation of L luminal diameter culture were compared. A cell concentration of 4 x 10(6) cells/well gave an optimal stimulation index (S.I.) of 121.3, with 50 micrograms/ml of concanavalin A (Con A), whereas cell concentration of 5 x 10(5) and concanavalin concentration of 100 micrograms/ml gave the lowest stimulation of 1.61. Good stimulation was observed in almost all cultures incubated for 48 hrs and labelled with 1 Mci triated thymidine (3HTdr) for the last 8 hrs. The highest S.I. were in the range of 66.3 to 121.3 (73,223 to 133,893 counts per minute) for the pig lymphocytes derived from whole blood culture.
Onyekaba
C
C
Department of Microbiology, School of Medicine, University of Minnesota, Minneapolis 55455.
D000818:Animals; D002470:Cell Survival; D002499:Centrifugation, Density Gradient; D003208:Concanavalin A; D007958:Leukocyte Count; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology*; D013552:Swine / Q000097:blood / Q000276:immunology*
D003208:Concanavalin A