A stable isotope assay for phenprocoumon and its metabolites.
A gas chromatographic/mass spectrometric assay for quantifying phenprocoumon and its 4'-, 6-, 7- and 8-hydroxy metabolites in microsomal preparations is described. This assay which uses deuterium-labeled analogs of the phenprocoumon metabolites as internal standards has a lower limit of quantitation of 20 ng ml-1. Diazomethane is used to derivatize both metabolites and parent compound yielding along with the expected 4-methoxy derivative a minor amount of the 2-methoxychromone. Resolution of the methylated metabolites is accomplished by capillary gas chromatography.
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D015110:4-Hydroxycoumarins / Q000032:analysis*; D000818:Animals; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D007668:Kidney / Q000166:cytology / Q000378:metabolism; D008168:Lung / Q000166:cytology / Q000378:metabolism; D008861:Microsomes / Q000032:analysis / Q000378:metabolism; D008862:Microsomes, Liver / Q000032:analysis / Q000378:metabolism; D010644:Phenprocoumon / Q000032:analysis* / Q000378:metabolism; D051381:Rats
D015110:4-Hydroxycoumarins; D010644:Phenprocoumon