title: [Tissue distribution of beta-lactam antibiotics, cefotiam and cefmenoxime in the lungs of sheep].
abstract: The present study was performed to investigate the distribution of beta-lactam antibiotics, cefotiam (CTM) and cefmenoxime (CMX) in pulmonary tissue of sheep. The animals were prepared to form chronic lung-lymph fistula for the collection of lung lymph. CTM and CMX were administered bolus-intravenously at doses of 20 mg/kg and 40 mg/kg, respectively, and serum and lymph levels of each drug were measured by bioassay method. Antibiotic levels of serum or lymph increased to a peak within 15 minutes after injection and then decreased rapidly. Measurable concentrations persisted for 240 minutes after the injection. Ratios of lung lymph to serum concentrations of CTM and CMX within 1 hour after the injection ranged 0.7 to 1.3, and 0.9 to 1.3, respectively. In addition, CMX levels in serum, lung lymph and tissues of both right and left lung were compared in anesthetized sheep to which CMX 50 mg/kg was given. Ratios of lung lymph and tissue concentrations of CMX in right and left lung to serum concentration were 0.76, 0.14 and 0.13, respectively. These results indicate that CTM and CMX were well distributed in interstitial fluid (lung lymph), and the levels of CMX in tissues of both right and left lung were markedly lower than those of lung lymph.
authors.affiliation: Department of Internal Medicine, School of Medicine, Shinshu University.
authors.forename: K
authors.initials: K
authors.lastname: Hirai
authors.forename: T
authors.initials: T
authors.lastname: Kobayashi
authors.forename: H
authors.initials: H
authors.lastname: Kobayashi
authors.forename: T
authors.initials: T
authors.lastname: Shibamoto
authors.forename: K
authors.initials: K
authors.lastname: Kubo
authors.forename: K
authors.initials: K
authors.lastname: Kitazawa
authors.forename: H
authors.initials: H
authors.lastname: Yagi
authors.forename: S
authors.initials: S
authors.lastname: Kusama
mesh_terms: D000818:Animals; D015281:Cefmenoxime; D002439:Cefotaxime / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000097:blood / Q000493:pharmacokinetics; D015310:Cefotiam; D007275:Injections, Intravenous; D008168:Lung / Q000378:metabolism*; D008196:Lymph / Q000378:metabolism
chemical_list: D015310:Cefotiam; D015281:Cefmenoxime; D002439:Cefotaxime