Identification of Mycobacterium bovis isolates using a monoclonal antibody.
A rapid immunoperoxidase slide assay for the identification of Mycobacterium bovis culture isolates is described. The monoclonal antibody used in this assay is specific for the M. tuberculosis complex of organisms. All M. bovis isolates tested, including 151 separate field isolates of M. bovis were positive as were 11 out of 12 M. tuberculosis strains and 4 out of 6 Bacillus Calmette Guerin (BCG) strains. One strain each of M. africanum and M. microti was negative. This assay provides a considerable improvement in both time and expense over the conventional methods of biochemical typing of M. bovis.
Corner
L A
LA
CSIRO, Division of Animal Health, Animal Health Research Laboratory, Parkville, Vic, Australia.
John
M
M
Bundesen
P G
PG
Wood
P R
PR
D000911:Antibodies, Monoclonal; D001681:Biological Assay; D009163:Mycobacterium bovis / Q000302:isolation & purification*; D009169:Mycobacterium tuberculosis / Q000276:immunology*
D000911:Antibodies, Monoclonal