title: The isolation and characterization of cisplatin-resistant rat ovarian cancer cells.
abstract: A rat ovarian cancer cell subline (Cis-Ptr), which became approximately 20-fold resistant to cisplatin, was developed after continuous exposure of the cisplatin-sensitive parent cell line (ROT68/C1) to increasing doses of the drug in vitro. Both the ROT68/C1 and Cis-Ptr cells were tumorigenic in isologous (Sprague-Dawley strain) newborn rats and only the tumors developed by inoculation of Cis-Ptr cells showed resistance to cisplatin in vivo. Resistance towards cisplatin was accompanied by phenotypic changes of the undifferentiated adenocarcinoma cells including enlargement of the cell and nucleus and a slower growth rate both in vitro and in vivo. Compared to the ROT68/C1 cells, the Cis-Ptr showed an early recovery of DNA synthetic activity after exposure to cisplatin. Cross-resistance of the Cis-Ptr cells was found only to a cisplatin analog, carboplatin. These results suggest that our cisplatin resistant rat ovarian cancer cells are useful in the investigation of the characteristics and mechanisms of cisplatin resistance.
authors.affiliation: Department of Obstetrics and Gynecology, Chiba University School of Medicine, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Sekiya
authors.forename: T
authors.initials: T
authors.lastname: Oosaki
authors.forename: S
authors.initials: S
authors.lastname: Andoh
authors.forename: N
authors.initials: N
authors.lastname: Suzuki
authors.forename: K
authors.initials: K
authors.lastname: Nagao
authors.forename: H
authors.initials: H
authors.lastname: Takamizawa
mesh_terms: D000230:Adenocarcinoma / Q000188:drug therapy / Q000473:pathology; D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002945:Cisplatin / Q000627:therapeutic use*; D004273:DNA, Neoplasm / Q000096:biosynthesis; D004351:Drug Resistance; D005260:Female; D010051:Ovarian Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D051381:Rats; D011919:Rats, Inbred Strains; D014407:Tumor Cells, Cultured / Q000187:drug effects
chemical_list: D004273:DNA, Neoplasm; D002945:Cisplatin