Improved procedure for determination of serum lipid-associated sialic acid: application for early diagnosis of colorectal cancer.
We have developed a simple and reliable procedure for determining serum levels of lipid-associated sialic acid (LASA) in crude preparations. This method extracts essentially all gangliosides, excludes glycoprotein-bound sialic acid, and gives LASA values (0.5-1.0 mg/dL) in good agreement with values for isolated serum gangliosides. The procedure was used to determine serum levels of LASA in patients with colorectal cancer, in patients with nonmalignant diseases (pathological control subjects), and in normal control subjects. The results indicated that the percentages of total sialic acid (TSA) comprised by LASA (LASA/TSA X 100) were elevated in patients with the earliest stages of colorectal cancer, compared with percentages in normal control subjects (P less than .001) and pathological control subjects (P less than .01).
Tautu
C
C
Department of Chemistry, Lehigh University, Bethlehem, PA 18015.
Verazin
G
G
Prorok
J J
JJ
Alhadeff
J A
JA
D000328:Adult; D000368:Aged; D015179:Colorectal Neoplasms / Q000097:blood / Q000175:diagnosis*; D005260:Female; D005732:Gangliosides / Q000097:blood; D006801:Humans; D008055:Lipids / Q000097:blood*; D008297:Male; D008875:Middle Aged; D019158:N-Acetylneuraminic Acid; D012794:Sialic Acids / Q000097:blood*
D005732:Gangliosides; D008055:Lipids; D012794:Sialic Acids; D019158:N-Acetylneuraminic Acid