title: Synthetic atrial natriuretic factor induces release (possibly receptor-mediated) of vasopressin from rat posterior pituitary.
abstract: The synthetic fragment (Arg 101-Tyr 126) of atrial natriuretic factor (ANF) induces release of arginine vasopressin from the isolated posterior lobe of the rat hypophysis in vitro. At a physiological concentration (3 X 10(-10)M) ANF was three times more effective than 61 mM KCL. In vitro binding studies with 125I-ANF revealed the presence of high affinity receptor sites displaying a pK = 9.9, a Kd = 0.14 nM, a Bmax = 20 fmol/posterior lobe and and IC50 = 200 pM. These results suggest that arginine vasopressin release by synthetic atrial natriuretic factor may be receptor mediated.
authors.forename: P
authors.initials: P
authors.lastname: Januszewicz
authors.forename: J
authors.initials: J
authors.lastname: Gutkowska
authors.forename: A
authors.initials: A
authors.lastname: De Lean
authors.forename: G
authors.initials: G
authors.lastname: Thibault
authors.forename: R
authors.initials: R
authors.lastname: Garcia
authors.forename: J
authors.initials: J
authors.lastname: Genest
authors.forename: M
authors.initials: M
authors.lastname: Cantin
mesh_terms: D000818:Animals; D001127:Arginine Vasopressin / Q000378:metabolism*; D009320:Atrial Natriuretic Factor; D004305:Dose-Response Relationship, Drug; D005260:Female; D009124:Muscle Proteins / Q000494:pharmacology*; D010904:Pituitary Gland, Posterior / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D009124:Muscle Proteins; C472610:atrial natriuretic peptide, rat; D001127:Arginine Vasopressin; D009320:Atrial Natriuretic Factor