Stimulator requirements for primed alloreactive T cells: macrophages and dendritic cells activate T cells across all genetic disparities.
The cellular requirements for stimulating primed alloreactive T cells have been investigated. In vitro-primed secondary alloreactive cells, long-term lines, and Ly 1+2- noncytolytic clones which reacted with allo-H-2K, D, or Mls (M locus) antigens were tested. The data indicated that a specialized antigen-presenting cell such as a macrophage or a dendritic cell was required for stimulating primed alloreactive cells across all the genetic disparities tested. B and T lymphocytes were ineffective stimulators. The stimulator requirement for secondary and Ly 1+2- clone responses was heterogeneous, since both macrophages and dendritic cells were effective stimulators. Thus, the allostimulator requirement for inducing proliferation and mediator secretion by the primed T-cell populations closely paralleled the requirement for stimulating unprimed populations. The only exception found was the peritoneal washout population, which did not stimulate a primary response but did stimulate secondary responses. The failure of peritoneal macrophages to stimulate a primary response was shown to be due to an inhibitory pathway which did not occur when the responding population was alloantigen primed.
Sunshine
G H
GH
Mitchell
T J
TJ
Czitrom
A A
AA
Edwards
S
S
Glasebrook
A L
AL
Kelso
A
A
MacDonald
H R
HR
D000818:Animals; D000911:Antibodies, Monoclonal / Q000502:physiology; D000938:Antigen-Presenting Cells / Q000145:classification* / Q000276:immunology; D001667:Binding, Competitive; D002448:Cell Adhesion; D002999:Clone Cells / Q000276:immunology; D006183:H-2 Antigens / Q000235:genetics*; D006238:Haploidy; D064532:Histocompatibility Antigen H-2D; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D008264:Macrophages / Q000145:classification / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D010529:Peritoneal Cavity / Q000166:cytology; D011947:Receptors, Antigen, B-Cell; D013154:Spleen / Q000166:cytology*; D013601:T-Lymphocytes / Q000276:immunology*
D000911:Antibodies, Monoclonal; D006183:H-2 Antigens; D064532:Histocompatibility Antigen H-2D; D000949:Histocompatibility Antigens Class II; D011947:Receptors, Antigen, B-Cell