An improved method of purification of transforming growth factor, type beta from platelets.
Platelets contain high concentrations of TGF-beta and for this reason have been the primary source of materials for its purification. The yield from acid-ethanol extracts of platelets has been reported to be 0.5 microgram/unit of platelets. We have developed a method which yields approximately 2 micrograms/unit of platelets from acid-ethanol extracts. The use of an ion-exchange column followed by RPLC results in a homogeneous product in less time and with fewer manipulations than the previously published methods. A tritiated thymidine incorporation assay using the CC164 cell line is described for the quantitation of TGF-beta.
Cone
J L
JL
Otsuka Pharmaceutical Company Ltd., Rockville, Maryland 20850.
Brown
D R
DR
DeLarco
J E
JE
D001792:Blood Platelets / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D000431:Ethanol; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010455:Peptides / Q000097:blood / Q000302:isolation & purification*; D015291:Transforming Growth Factors
D010455:Peptides; D000431:Ethanol; D015291:Transforming Growth Factors