[Electrophoretic evaluation of multiple molecular forms of enzymes and their ratios using alcohol dehydrogenase from mammalian liver cytosol as an example].
Zymograms of alcohol dehydrogenase from liver cytosol of horse, rat, pig, bull and calf were studied in order to evaluate the advantages and limitations of polyacrylamide gel disc electrophoresis in estimation of multiple molecular forms of the enzyme and their ratios. The zymograms of these animal species differed in amount of zones of the enzymatic activity, in their relative electrophoretic mobility (Rf) and in intensity of staining. The amount of zones and intensity of staining depended on the time of incubation and pH value. No direct correlation could be found between the period of incubation and intensity of the zones colouring, which was due to a non-linear type of dynamics of the reaction products accumulation. As a precondition for correct evaluation of the zymograms results of enzymological estimations (involving pH optima, temperature, saturating concentrations for substrates and coenzymes) were essential.
Sudovtsov
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D000426:Alcohol Dehydrogenase; D000429:Alcohol Oxidoreductases / Q000378:metabolism*; D000818:Animals; D003600:Cytosol / Q000201:enzymology; D004591:Electrophoresis, Polyacrylamide Gel; D006863:Hydrogen-Ion Concentration; D007527:Isoenzymes / Q000378:metabolism*; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008322:Mammals; D013045:Species Specificity
D007527:Isoenzymes; D000429:Alcohol Oxidoreductases; D000426:Alcohol Dehydrogenase
K voprosu ob élektroforeticheskoĭ otsenke mnozhestvennykh molekuliarnykh form fermentov i ikh sootnosheniiakh na primere alkogol'degidrogenazy iz tsitozolia pecheni mlekopitaiushchikh.