title: [Enzymatic cleavage of human neurophysin into 2 sub-domains containing 3 and 4 disulfides].
abstract: Native human MSEL-neurophysin has been subjected to trypsin hydrolysis. Because of a change of the bond Lys59-Pro60 found in other mammalian neurophysins into Lys59-Ala60 in human protein, a peculiar cleavage has occurred in the latter, leading to a split of the molecule into two halves. The N-terminal sub-domain contains 4 disulfide bridges whereas the C-terminal one possesses 3 disulfide bridges.
authors.affiliation: Laboratoire de Chimie biologique, Université Pierre-et-Marie-Curie, Paris.
authors.forename: J
authors.initials: J
authors.lastname: Chauvet
authors.forename: G
authors.initials: G
authors.lastname: Michel
authors.forename: M T
authors.initials: MT
authors.lastname: Chauvet
authors.forename: R
authors.initials: R
authors.lastname: Acher
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002845:Chromatography; D002851:Chromatography, High Pressure Liquid; D004220:Disulfides / Q000378:metabolism*; D006801:Humans; D006868:Hydrolysis; D008969:Molecular Sequence Data; D009481:Neurophysins / Q000378:metabolism*; D010446:Peptide Fragments / Q000302:isolation & purification / Q000378:metabolism*; D014357:Trypsin / Q000378:metabolism*
chemical_list: D000596:Amino Acids; D004220:Disulfides; D009481:Neurophysins; D010446:Peptide Fragments; D014357:Trypsin
vernacular_title: Dissection enzymatique de la neurophysine humaine en deux sous-domaines contenant 4 et 3 ponts disulfures.