HDL particle associated proteins in plasma and cerebrospinal fluid: identification and partial sequencing.
The proteins from plasma HDL particles isolated by immunoaffinity chromatography on anti-apolipoprotein A-1 affinity columns have been analysed and purified by high resolution two dimensional gel electrophoresis. Two of the lipoprotein-associated proteins found in the HDL plasma fraction, previously referred to as NA1 and NA2, have also been found in cerebrospinal fluid. After separation by 2DGE, these two proteins were transferred to PVDF membranes, stained and cut out for N-terminal sequencing. The partial sequences (11 and 13 amino acids) obtained for the two HDL particle associated proteins do not match any of those included in the December 1987 National Biomedical Research Foundation (NBRF) database, and there are no significant sequence similarities.
Hochstrasser
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AC
Biochemical Genetics Section, National Institute of Mental Health, Bethesda, Maryland 20892.
James
R W
RW
Martin
B M
BM
Harrington
M
M
Hochstrasser
D
D
Pometta
D
D
Merril
C R
CR
D000595:Amino Acid Sequence; D001798:Blood Proteins / Q000302:isolation & purification*; D002556:Cerebrospinal Fluid Proteins / Q000302:isolation & purification*; D002846:Chromatography, Affinity / Q000379:methods; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D015151:Immunoblotting; D008075:Lipoproteins, HDL / Q000032:analysis*; D008969:Molecular Sequence Data; D009439:Neuraminidase
D001798:Blood Proteins; D002556:Cerebrospinal Fluid Proteins; D008075:Lipoproteins, HDL; D009439:Neuraminidase