Different mechanisms account for the relative resistance of KG-1 and HL-60 cell lines to retrovirus infection.
I infected three different human leukemic cell lines (K562, KG-1, and HL-60) with an amphotropic retrovirus vector (designated PA317/N2) which confers G418 resistance and contains the Moloney murine leukemia virus long terminal repeat. Compared with K562 cells, both KG-1 and HL-60 cells were relatively resistant to infection with this retrovirus vector. In HL-60 cells, this resistance appeared to result from diminished viral DNA synthesis, while in KG-1 cells there was a block to the genomic integration of the viral DNA.
Collins
S J
SJ
Molecular Medicine Program, Fred Hutchinson Cancer Research Center, Seattle, Washington 98104.
D002460:Cell Line; D005814:Genes, Viral; D005822:Genetic Vectors; D006412:Hematopoietic Stem Cells; D006801:Humans; D008979:Moloney murine leukemia virus / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid; D012190:Retroviridae / Q000472:pathogenicity*; D014162:Transfection