title: Inactivation of soybean lipoxygenase 1 by 12-iodo-cis-9-octadecenoic acid.
abstract: 12-Iodo-cis-9-octadecenoic acid (12-IODE) is a time-dependent, irreversible inactivator of soybean lipoxygenase 1. The rate of inactivation is independent of 12-IODE concentration above 20 microM and is half-maximal at about 4 microM. Inactivation by 12-IODE requires lipid hydroperoxide, which must be present even after the initial oxidation of the iron in the enzyme from ferrous to ferric. Inactivation by 12-IODE is also dependent on O2. These findings suggest that 12-IODE is converted by the enzyme into a more reactive species, which is responsible for inactivation. No inactivation has been detected with 12-iodooctadecanoic acid, 12-bromo-cis-9-octadecenoic acid, 12-iodo-trans-9-octadecenoic acid, or a mixture of stereoisomers of 9,11-octadecadienoic acid.
authors.affiliation: Department of Chemistry, Bucknell University, Lewisburg, Pennsylvania 17837.
authors.forename: S A
authors.initials: SA
authors.lastname: Rotenberg
authors.forename: A M
authors.initials: AM
authors.lastname: Grandizio
authors.forename: A T
authors.initials: AT
authors.lastname: Selzer
authors.forename: C H
authors.initials: CH
authors.lastname: Clapp
mesh_terms: D000332:Aerobiosis; D000693:Anaerobiosis; D007202:Indicators and Reagents; D007700:Kinetics; D016859:Lipoxygenase Inhibitors; D009829:Oleic Acids / Q000138:chemical synthesis / Q000494:pharmacology*; D010944:Plants / Q000201:enzymology*; D013025:Glycine max
chemical_list: D007202:Indicators and Reagents; D016859:Lipoxygenase Inhibitors; D009829:Oleic Acids; C059121:12-iodo-9-octadecenoic acid