Coexistence of TRH with other neuroactive substances in the rat central nervous system.
Colocalization of thyrotropin-releasing hormone-like immunoreactivity with other neuroactive substances was examined immunohistochemically in colchicine-treated rat brains using double-staining or elution-restaining methods. Thyrotropin-releasing hormone-like immunoreactivity was shown to be located in the same neurons as: 1. enkephalin-, gamma-amino butyric acid- and tyrosine hydroxylase-, but not somatostatin-like immunoreactivity in the glomerular layer of the olfactory bulb 2. oxytocin- and cholecystokinin-, but not vasopressin-like immunoreactivity in the supraoptic nucleus 3. cholecystokinin-like immunoreactivity in posterior pituitary 4. enkephalin-like immunoreactivity in the perifornical area of the hypothalamus and 5. neuropeptide Y- and neurotensin-like immunoreactivity in the periaqueductal central grey. These findings provide further examples of coexistence of thyrotropin-releasing hormone with classical neurotransmitters and/or peptides in the rat central nervous system.
Tsuruo
Y
Y
Department of Histology, Karolinska Institute, Stockholm, Sweden.
Ceccatelli
S
S
Villar
M J
MJ
Hökfelt
T
T
Visser
T J
TJ
Terenius
L
L
Goldstein
M
M
Brown
J C
JC
Buchan
A
A
Walsh
J
J
D000818:Animals; D002490:Central Nervous System / Q000166:cytology / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D009479:Neuropeptides / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013973:Thyrotropin-Releasing Hormone / Q000378:metabolism*
D009479:Neuropeptides; D013973:Thyrotropin-Releasing Hormone