title: Sublines of the Burkitt line Daudi, selected on the basis of reactivity with soybean agglutinin, differ in tumorigenicity in athymic mice.
abstract: Two subpopulations were isolated on the basis of soybean agglutinin (SBA) binding, from the human Burkitt lymphoma line Daudi. The low- and high-binder sublines maintained this characteristic in continuous passages. Their surface marker profiles, antibodies, scanning electron microscope (SEM), cytochemical reactions and binding of other lectins (concanavalin A and wheatgerm agglutinin) were not different. They differed, however, in growth potential in athymic mice. The low-binder subline had lower frequency of takes, tumor weight and volume, and did not metastasize as compared to the high-binder subline. However, the reaction with F-SBA of all the tumor cells examined was strong (greater than 70%), indicating in vivo selection and tumor development of high binder cells.
authors.affiliation: Department of Medicine A, Hadassah University Hospital, Jerusalem, Israel.
authors.forename: H
authors.initials: H
authors.lastname: Ben-Bassat
authors.forename: S
authors.initials: S
authors.lastname: Weksler-Zangen
authors.forename: D
authors.initials: D
authors.lastname: Shouval
mesh_terms: D000818:Animals; D002051:Burkitt Lymphoma / Q000276:immunology*; D006801:Humans; D037102:Lectins; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D037121:Plant Lectins; D030262:Soybean Proteins; D014407:Tumor Cells, Cultured / Q000276:immunology; D010835:Phytohemagglutinins
chemical_list: D037102:Lectins; D037121:Plant Lectins; D030262:Soybean Proteins; C028824:soybean lectin; D010835:Phytohemagglutinins