title: Studies on the replication of bacteriophage Cp-1 DNA in Streptococcus pneumoniae.
abstract: The DNA of bacteriophage Cp-1 replicates at optimal conditions when Cp-1-infected Streptococcus pneumoniae was incubated at 30 degrees C. The in vitro formation of the initiation complex between the terminal protein and 5'-dAMP was only partially inhibited at 37 degrees C whereas an almost complete inhibition of the DNA replication was found at this temperature in vivo. Aphidicolin inhibited the multiplication of phage Cp-1 but not that of Dp-4. This drug did not affect the in vitro formation of the initiation complex but seems to affect extensive Cp-1 DNA replication in vivo.
authors.affiliation: Centro de Investigaciones Biológicas, CSIC. Velázquez, Madrid, Spain.
authors.forename: E
authors.initials: E
authors.lastname: García
authors.forename: C
authors.initials: C
authors.lastname: Ronda
authors.forename: P
authors.initials: P
authors.lastname: García
authors.forename: R
authors.initials: R
authors.lastname: López
mesh_terms: D016590:Aphidicolin; D001435:Bacteriophages / Q000187:drug effects / Q000502:physiology*; D004261:DNA Replication / Q000187:drug effects; D004279:DNA, Viral / Q000096:biosynthesis; D004268:DNA-Binding Proteins / Q000378:metabolism; D003838:Deoxyadenine Nucleotides / Q000378:metabolism; D004224:Diterpenes / Q000494:pharmacology; D013296:Streptococcus pneumoniae / Q000187:drug effects / Q000378:metabolism*; D013696:Temperature; D014764:Viral Proteins / Q000378:metabolism; D014779:Virus Replication / Q000187:drug effects
chemical_list: D004279:DNA, Viral; D004268:DNA-Binding Proteins; D003838:Deoxyadenine Nucleotides; D004224:Diterpenes; D014764:Viral Proteins; C048231:terminal protein, Streptococcus phage Cp-1; D016590:Aphidicolin; C012873:2'-deoxy-5'-adenosine monophosphate