Hybridoma fusion cell lines contain an aberrant kappa transcript.
The V region sequence of a non-productive kappa transcript from two myeloma fusion partners has been determined. This transcript has an aberrant VJ recombination site resulting in a translation stop site at position 105. It is variably expressed in hybridomas made from all fusion partners derived from the original MOPC-21 tumor. The amount of this transcript may greatly exceed levels of the productive light chain mRNA.
Carroll
W L
WL
Department of Medicine, Stanford University School of Medicine, CA 94305.
Mendel
E
E
Levy
S
S
D000818:Animals; D000912:Antibodies, Neoplasm / Q000235:genetics; D001483:Base Sequence; D002460:Cell Line; D005803:Genes, Immunoglobulin; D006801:Humans; D006825:Hybridomas / Q000276:immunology*; D007145:Immunoglobulin kappa-Chains / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D009101:Multiple Myeloma / Q000235:genetics; D014158:Transcription, Genetic
D000912:Antibodies, Neoplasm; D007145:Immunoglobulin kappa-Chains