title: Preparation of high-titred antisera for sigma virus strains from Hawaii and France and their use in neutralization tests in vitro and in vivo.
abstract: Antisera were prepared in rabbits against two strains of sigma virus, from France and Hawaii respectively, using purified virus from cell cultures. All antisera had the same titre by immunofluorescence, 1/400 or 1/800, whether tested against the homologous or the heterologous virus. An antiserum prepared with the French strain neutralized the Hawaiian virus at a dilution of 1/800 both in vitro and in vivo. The Hawaiian virus was not neutralized to a significant extent by reference mouse ascitic fluids for 26 different vertebrate rhabdoviruses.
authors.affiliation: Pacific Biomedical Research Center, University of Hawaii, Honolulu 96822.
authors.forename: M C
authors.initials: MC
authors.lastname: Vazeille
authors.forename: L
authors.initials: L
authors.lastname: Rosen
mesh_terms: D000330:Aedes; D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology*; D002478:Cells, Cultured; D004331:Drosophila melanogaster; D005602:France; D006254:Hawaii; D008854:Microscopy, Electron; D009500:Neutralization Tests; D012209:Rhabdoviridae / Q000276:immunology* / Q000648:ultrastructure
chemical_list: D000914:Antibodies, Viral