Immunosuppression by cell-free translation products from monoclonal antigen-specific suppressor T cell mRNA.
Polypeptides synthesized in a rabbit reticulocyte lysate system directed by mRNA from the T cell line LH8-105, obtained by radiation leukemia virus-induced transformation of hen egg-white lysozyme (HEL)-specific suppressor T lymphocytes, are able, when injected into mice, to specifically suppress the antibody response and delayed-type hypersensitivity to HEL. The suppressive activity exerted by in vitro translated proteins appears to be independent from post-translational modifications. These in vitro translated polypeptides display fine antigenic specificity in immunosuppression and bind to HEL but not to the closely related ring-necked pheasant egg-white lysozyme immunosorbents. Suppressive molecules obtained by cell-free translation of LH8-105 mRNA or by culture supernatant of LH8-105 cells display, by gel filtration, a similar molecular mass of about 82-90 kDa.
Ricciardi-Castagnoli
P
P
Robbiati
F
F
Barbanti
E
E
Colizzi
V
V
Pini
C
C
De Santis
R
R
Doria
G
G
Adorini
L
L
D000818:Animals; D000917:Antibody Formation; D000941:Antigens / Q000276:immunology*; D002474:Cell-Free System; D006968:Hypersensitivity, Delayed; D007165:Immunosuppression Therapy; D066298:In Vitro Techniques; D051379:Mice; D008970:Molecular Weight; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000276:immunology*; D011817:Rabbits; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*
D000941:Antigens; D012333:RNA, Messenger