Effect of 2,4,6-trinitrophenol on catecholamine secretion from perfused bovine adrenal glands.
2,4,6-Trinitrophenol (PA) evoked a prolonged catecholamine (CA) secretion from perfused bovine adrenal glands. The PA-evoked CA secretion was concentration-dependent, required the presence of extracellular calcium and resulted from a direct action of PA on the chromaffin cells. Furthermore, PA reduced Mg2+-ATPase activities in the plasma membrane-rich microsome and granule-rich fraction from the adrenal medulla. These results indicate that PA evokes CA secretion through the actions on both the chromaffin cell membranes and granule membranes.
Yamanaka
K
K
Yamada
S
S
Hayashi
E
E
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000311:Adrenal Glands / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D017301:Ca(2+) Mg(2+)-ATPase; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D002395:Catecholamines / Q000378:metabolism*; D002417:Cattle; D002462:Cell Membrane / Q000201:enzymology; D003594:Cytoplasmic Granules / Q000201:enzymology; D066298:In Vitro Techniques; D008861:Microsomes / Q000201:enzymology; D010851:Picrates / Q000494:pharmacology*; D013997:Time Factors
D002395:Catecholamines; D010851:Picrates; C005858:picric acid; D000251:Adenosine Triphosphatases; D017301:Ca(2+) Mg(2+)-ATPase; D000252:Calcium-Transporting ATPases