Quantitative plating of Histoplasma capsulatum without addition of conditioned medium or siderophores.
We have formulated two defined media which yield excellent plating efficiency of the yeast phase of Histoplasma capsulatum. Neither requires the addition of conditioned medium or purified siderophores, although the simpler of the two media includes hemin as a source of solubilized iron. Agarose, rather than conventional agar, is the solidifying agent for both media. Plating efficiency usually exceeds 90% with both North American and Central American isolates of H. capsulatum.
Worsham
P L
PL
Department of Microbiology and Immunology, Washington University School of Medicine, St Louis, Missouri 63110.
Goldman
W E
WE
D003470:Culture Media; D006427:Hemin; D006658:Histoplasma / Q000254:growth & development*; D007502:Iron Chelating Agents; D012685:Sepharose
D003470:Culture Media; D007502:Iron Chelating Agents; D006427:Hemin; D012685:Sepharose