The beta 1 domain of the mouse E beta chain is important for restricted antigen presentation to helper T-cell hybridomas.
We have constructed a hybrid E beta gene by replacing the second exon of the Ed beta gene (which encodes the majority of the beta 1 protein domain) with the corresponding exon from the Eb beta gene. The hybrid gene has been introduced into a d haplotype host, the lymphoma beta-cell line A20-2J, and an E alpha E beta dimer composed of the endogenous Ed alpha chain and the product of the hybrid Ed beta/Eb beta gene was immunoprecipitated from extracts of transfected cells with an Ed alpha Eb beta-specific monoclonal antibody. Transfected cells have acquired the ability to present antigen to Ed alpha Eb beta-restricted helper T-cell hybridomas, indicating that the second exon of the gene for the E beta chain encodes sequences required for the restricted recognition of the antigen-presenting cell by the class II-restricted responder T-cell.
Folsom
V
V
Gay
D
D
Tonegawa
S
S
D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology*; D001483:Base Sequence; D004274:DNA, Recombinant; D006649:Histocompatibility Antigens / Q000235:genetics* / Q000276:immunology; D006825:Hybridomas / Q000276:immunology; D051379:Mice; D008968:Molecular Conformation; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*
D004274:DNA, Recombinant; D006649:Histocompatibility Antigens