Genetic analysis in Streptomyces ambofaciens.
A chromosomal linkage map of ten markers was established for Streptomyces ambofaciens by the four-factor cross method and allele-gradient analysis. Mutants were obtained by nitrous acid treatment as well as UV mutagenesis. The fertility of crosses was enhanced over 100-fold by pSAM2, a plasmid present in some strains of S. ambofaciens, and over 1000-fold by the conjugative plasmid pIJ303.
Smokvina
T
T
Laboratoire de Biologie et Génétique Moléculaire, Université de Paris-Sud, Orsay, France.
Francou
F
F
Luzzati
M
M
D000483:Alleles; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D003434:Crossing Over, Genetic; D005838:Genotype; D009154:Mutation; D011995:Recombination, Genetic; D013302:Streptomyces / Q000235:genetics*