Metabolism of C26 bile alcohols in the bullfrog, Rana catesbeiana.
Metabolism of C26 bile alcohols in the bullfrog, Rana catesbeiana, was studied. [24-14C]-24-Dehydro-26-deoxy-5 beta-ranol (3 alpha,7 alpha,12 alpha-trihydroxy-27-nor-5 beta-cholestan-24-one) was chemically synthesized from [24-14C]cholic acid and incubated with bullfrog liver homogenate fortified with NADPH. 24-Dehydro-26-deoxy-5 beta-ranol was shown to be converted into both 26-deoxy-5 beta-ranol and 24-epi-26-deoxy-5 beta-ranol [(24S)- and (24R)-27-nor-5 beta-cholestane-3 alpha,7 alpha,12 alpha,24-tetrols] in addition to 5 beta-ranol [(24R)-27-nor-5 beta-cholestane-3 alpha,7 alpha,12 alpha,24,26-pentol], which is the major bile alcohol of the bullfrog. [24-3H]-26-Deoxy-5 beta-ranol and [24-3H]-24-epi-26-deoxy-5 beta-ranol were prepared from 24-dehydro-26-deoxy-5 beta-ranol by reduction with sodium [3H] borohydride and administered respectively to two each of four bullfrogs by intraperitoneal injection. After 24 h, labeled 5 beta-ranol was isolated from the bile of the bullfrogs that received [24-3H]-26-deoxy-5 beta-ranol. In contrast little if any radioactivity could be detected in 5 beta-ranol or its 24-epimer after administration of [24-3H]-24-epi-26-deoxy-5 beta-ranol.
Noma
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Hiroshima Prefectural Research Center for Environmental Science, Japan.
Kihira
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K
Kuramoto
T
T
Hoshita
T
T
D000111:Acetylcysteine / Q000494:pharmacology; D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology; D004156:Catechol Oxidase / Q000378:metabolism*; D002777:Cholestanols / Q000378:metabolism*; D004958:Estradiol / Q000031:analogs & derivatives* / Q000032:analysis; D002393:Estrogens, Catechol; D005978:Glutathione / Q000494:pharmacology; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism*; D014442:Monophenol Monooxygenase / Q000378:metabolism*; D011892:Rana catesbeiana; D051381:Rats; D014316:Tritium
D002777:Cholestanols; D002393:Estrogens, Catechol; D014316:Tritium; D004958:Estradiol; C014036:4-hydroxyestradiol; D004156:Catechol Oxidase; D014442:Monophenol Monooxygenase; D005978:Glutathione; D001205:Ascorbic Acid; D000111:Acetylcysteine