Polyacrylamide gel electrophoresis (PAGE) of whole-cell proteins of cutaneous Propionibacterium species.
Polyacrylamide gel electrophoresis (PAGE) was applied to the study of whole-cell proteins of cutaneous propionibacteria in an attempt to characterise possible protein patterns that may be typical for strains isolated from acne skin. Isolates were obtained from the faces of 33 individuals aged 7-16 years. Some of these subjects had apparently normal healthy skin, whereas others had acne vulgaris of varying severity. Twenty-five facial isolates of Propionibacterium acnes and eight of P. granulosum were studied. A further seven axillary strains of P. avidum were included for purely taxonomic interest. No particular protein pattern was characteristic of an isolate from acne skin; in fact the P. acnes strains from all sources appeared to be identical.
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D000152:Acne Vulgaris / Q000382:microbiology; D000293:Adolescent; D001426:Bacterial Proteins / Q000032:analysis*; D002648:Child; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D011424:Propionibacterium / Q000032:analysis* / Q000302:isolation & purification; D012867:Skin / Q000382:microbiology*; D013045:Species Specificity
D001426:Bacterial Proteins