High-performance liquid chromatographic determination of valproic acid in plasma using a micelle-mediated pre-column derivatization.
A method for the determination of valproic acid (2-propylpentanoic acid) in plasma by high-performance liquid chromatography (HPLC) after pre-column derivatization is described. The derivatization of valproic acid with a fluorophore and UV label, 4-bromomethyl-7-methoxycoumarin, is performed in plasma diluted with an aqueous micellar system. No extraction or solvent evaporation steps are required. The mechanism of the derivatization of the carboxylic acid is based on phase-transfer catalysis. The sample preparation, including the derivatization step, is rapid and very simple. The proposed HPLC-method was evaluated and compared with a standard immunological assay used for the determination of valproic acid in plasma.
van der Horst
F A
FA
Department of Pharmaceutical Analysis, Faculty of Pharmacy, University of Utrecht, The Netherlands.
Eikelboom
G G
GG
Holthuis
J J
JJ
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003374:Coumarins; D006801:Humans; D008823:Micelles; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D014635:Valproic Acid / Q000097:blood*
D003374:Coumarins; D008823:Micelles; C036126:4-bromomethyl-7-acetoxycoumarin; D014635:Valproic Acid