Monoclonal IgE-dependent eosinophil cytotoxicity to haptenated schistosomula of Schistosoma japonicum: enhancement of the cytotoxicity and expression of Fc receptors for IgE by Nippostrongylus brasiliensis infection.
To obtain direct evidence for the involvement of IgE antibodies in eosinophil-mediated killing of schistosomula of S. japonicum, dinitrophenylated (DNP) schistosomula pretreated with mouse monoclonal IgE antibodies were co-cultured with purified rat peritoneal eosinophils. It was found that the eosinophil-mediated adherence and damage to haptenated schistosomula were dependent on a monoclonal anti-DNP IgE antibody, but not on monoclonal anti-ovalbumin IgE antibody. Moreover, eosinophils from N. brasiliensis-infected rats demonstrated an enhanced ability in the IgE-dependent damage to DNP-schistosomula as compared with the cells from normal rats. The enhancement was associated with an increase in the proportion of eosinophils expressing Fc receptors for IgE.
Kojima
S
S
Yamamoto
N
N
Kanazawa
T
T
Ovary
Z
Z
D000818:Animals; D000911:Antibodies, Monoclonal / Q000502:physiology*; D002448:Cell Adhesion; D003602:Cytotoxicity, Immunologic; D004136:Dinitrobenzenes / Q000276:immunology; D004804:Eosinophils / Q000276:immunology* / Q000378:metabolism / Q000502:physiology; D006241:Haptens / Q000276:immunology; D007073:Immunoglobulin E / Q000378:metabolism / Q000502:physiology*; D051379:Mice; D009349:Nematode Infections / Q000276:immunology*; D009559:Nippostrongylus / Q000276:immunology; D051381:Rats; D011919:Rats, Inbred Strains; D011961:Receptors, Fc / Q000032:analysis*; D017455:Receptors, IgE; D012397:Rosette Formation; D012549:Schistosoma japonicum / Q000276:immunology; D012552:Schistosomiasis / Q000276:immunology* / Q000469:parasitology
D000911:Antibodies, Monoclonal; D004136:Dinitrobenzenes; D006241:Haptens; D011961:Receptors, Fc; D017455:Receptors, IgE; D007073:Immunoglobulin E