The gonadotrope polypeptide (GP 87) released from pituitary cells under luteinizing hormone-releasing hormone stimulation is a secretogranin II form.
Cultured gonadotrope cells from 14 day old female rat pituitaries have been shown to release a highly acidic protein when incubated with LHRH: the gonadotrope polypeptide (GP 87). Moreover, a new tyrosine-sulfated acidic protein, secretogranin II (Sg II), clearly distinct from the chromogranin species, was described in the secretory granule matrix of endocrine cells secreting peptide hormones by the regulated pathway. Recently, the release of Sg II from female rat pituitary stimulated by LHRH was demonstrated in vitro. Several physicochemical (Mr; pI) and biological (cellular localization in the pituitary; LHRH-stimulated release) properties are common to Sg II and GP 87. To verify if these 2 polypeptides are similar or distinct components, other physicochemical characteristics (heat-stability, sulfation, phosphorylation) were compared using isotope incorporation followed by either 1- or 2-dimensional polyacrylamide gel electrophoresis and autoradiography. Furthermore, the similarity of GP 87 to Sg II was studied by immunoblotting on nitrocellulose sheets following electrophoresis of intracellular and secreted proteins. Antisera raised against bovine Sg II (extracted from whole pituitaries) and against rat GP 87 (released into the medium of cultured pituitary cells stimulated by LHRH) were used. The overall data presented here suggest that GP 87 is the Sg II form contained in, and released by, gonadotrope cells.
Chanat
E
E
Laboratoire d'Endocrinologie Moléculaire, CNRS UA 256, Rennes, France.
Cozzi
M G
MG
Sion
B
B
de Monti
M
M
Zanini
A
A
Duval
J
J
D000818:Animals; D000937:Antigen-Antibody Reactions; D002478:Cells, Cultured; D002864:Chromogranins; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000494:pharmacology*; D008970:Molecular Weight; D010759:Phosphorus Isotopes; D010902:Pituitary Gland / Q000187:drug effects / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D013460:Sulfur Isotopes
D002864:Chromogranins; D010759:Phosphorus Isotopes; D011506:Proteins; D013460:Sulfur Isotopes; D007987:Gonadotropin-Releasing Hormone