title: Purification and characterization of an agglutinin from mucus of the snail Achatina fulica.
abstract: The mucus of the snail Achatina fulica shows the presence of an agglutinin that nonspecifically agglutinates human erythrocytes. The agglutinin has been purified by affinity chromatography using Sepharose 4B-hog gastric mucin as the affinity matrix. Homogeneity was checked by polyacrylamide gel electrophoresis, immunodiffusion, immunoelectrophoresis, and gel filtration. The agglutinin is a glycoprotein of native molecular weight 70,000. The isoelectric point of the protein was found to be 8.0. The predominant amino acids are aspartic acid and glutamic acid (or amides) and serine, which account for 32% of the total amino acid residues. The agglutinin has 10% carbohydrate (wt/wt) and the most abundant sugar is N-acetylglucosamine. The cd spectra of the agglutinin show the presence of random coil conformation. The inhibition of hemagglutination data indicates that the agglutinin is specific for beta glycosides of D-Gal and D-GalNAc.
authors.affiliation: Indian Institute of Chemical Biology, Calcutta.
authors.forename: D
authors.initials: D
authors.lastname: Mitra
authors.forename: M
authors.initials: M
authors.lastname: Sarkar
authors.forename: A K
authors.initials: AK
authors.lastname: Allen
mesh_terms: D000373:Agglutinins / Q000302:isolation & purification*; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002241:Carbohydrates / Q000032:analysis; D002850:Chromatography, Gel; D002942:Circular Dichroism; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000302:isolation & purification*; D006385:Hemagglutination Inhibition Tests; D005779:Immunodiffusion; D066298:In Vitro Techniques; D009093:Mucus / Q000032:analysis*; D012908:Snails
chemical_list: D000373:Agglutinins; D000596:Amino Acids; D002241:Carbohydrates; D006023:Glycoproteins