title: [Synthesis and secretion of the surface antigen from hepatitis B virus in animal cell cultures].
abstract: Stable mammalian cell lines synthesizing and secreting Hepatitis B surface particles have been obtained through genetic engineering techniques. These particles show by electron microscopy a size of 22 nm, they are structurally and immunochemically similar to the particles present in the plasma from chronic hepatitis B patients. Therefore these particles are an excellent source for the preparation of a vaccine against the virus.
authors.affiliation: Laboratorio de Biología Molecular, Instituto de Biotecnología BIOS-CHILE S.A., Santiago, Chile.
authors.forename: A E
authors.initials: AE
authors.lastname: De Ioannes
authors.forename: A
authors.initials: A
authors.lastname: Yudelevich
authors.forename: A
authors.initials: A
authors.lastname: Venegas
mesh_terms: D000818:Animals; D006224:Cricetinae; D005260:Female; D005818:Genetic Engineering; D006514:Hepatitis B Surface Antigens / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification; D051379:Mice; D008807:Mice, Inbred BALB C; D010053:Ovary / Q000166:cytology; D010957:Plasmids / Q000235:genetics*; D014162:Transfection
chemical_list: D006514:Hepatitis B Surface Antigens
vernacular_title: Síntesis y secreción del antígeno de superficie del virus de Hepatitis B en cultivo de células animales.