Effects of manno-1-deoxynojirimycin and 2,5-dihydroxymethyl-3,4-dihydroxypyrrolidine on N-linked oligosaccharide processing in intestinal epithelial cells.
The effects of manno-1-deoxynojirimycin (ManDJN) and 2,5-dihydroxymethyl-3,4-dihydroxypyrrolidine (DMDP) were compared in IEC-6 intestinal epithelial cells in culture. ManDJN caused complete inhibition of N-linked complex oligosaccharide synthesis whereas a maximum of 80% inhibition was obtained with DMDP. HPLC showed similar endo H-sensitive oligosaccharides for control and treated cells. ManDJN caused a large increase in the levels of labeled Man7-9 GlcNAc and a decrease in Man5GlcNAc. DMDP produced similar changes except that the increase in Man7-9GlcNAc was less pronounced and some increase in glucosylated oligosaccharides was observed. Since the major oligosaccharides found in DMDP-treated cells were non-glucosylated, its primary effect on complex oligosaccharide synthesis is not due to inhibition of glucosidases, in contrast to what has been reported for influenza virus-infected MDCK cells [(1984) J. Biol. Chem. 259, 12409-12413].
Romero
P A
PA
Friedlander
P
P
Fellows
L
L
Evans
S V
SV
Herscovics
A
A
D017485:1-Deoxynojirimycin; D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004848:Epithelium / Q000378:metabolism; D005944:Glucosamine / Q000031:analogs & derivatives / Q000494:pharmacology; D006023:Glycoproteins / Q000096:biosynthesis*; D050113:Imino Furanoses; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism; D008353:Mannitol / Q000031:analogs & derivatives; D009844:Oligosaccharides / Q000096:biosynthesis*; D011499:Protein Processing, Post-Translational / Q000187:drug effects*; D011759:Pyrrolidines / Q000494:pharmacology*; D051381:Rats; D013329:Structure-Activity Relationship
D000470:Alkaloids; D006023:Glycoproteins; D050113:Imino Furanoses; D009844:Oligosaccharides; D011759:Pyrrolidines; D017485:1-Deoxynojirimycin; D008353:Mannitol; C043121:2,5-dihydroxymethyl-3,4-dihydroxypyrrolidine; D005944:Glucosamine