title: [The effect of calcium and magnesium ions on the interaction of calcium-binding proteins parvalbumin and alpha-lactalbumin with dipalmitoylphosphatidylcholine vesicles].
abstract: Interactions of the calcium binding proteins, like parvalbumins pI 4.2 and p15.0 and bovine and human alpha-lactalbumins, with dipalmitoylphosphatidylcholine vesicles have been studied by means of scanning microcalorimetry and intrinsic tryptophan, tyrosine and phenylalanine fluorescence. The interactions are modulated by the Ca2+ and Mg2+ binding to the proteins and induce some changes in the physical properties of both the proteins and the liposomes. The liposomes increase the thermal stability of the Mg2+-loaded and metal-free parvalbumin. Ca2+-loaded alpha-lactalbumin interacts with the liposomes in its native state, while the metal-free protein binds to the liposomes mainly in its thermally denatured state. The interactions of both proteins with the liposomes affect the phase transition from gel to liquid-crystalline state in the liposomes. The results of the microcalorimetric and spectrofluorometric studies are corroborated by the data obtained by means of gel-chromatography on Sepharose 4B.
authors.forename: E A
authors.initials: EA
authors.lastname: Permiakov
authors.forename: D I
authors.initials: DI
authors.lastname: Kreĭmer
authors.forename: L P
authors.initials: LP
authors.lastname: Kalinichenko
authors.forename: V L
authors.initials: VL
authors.lastname: Shnyrov
mesh_terms: D015060:1,2-Dipalmitoylphosphatidylcholine; D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D002417:Cattle; D002850:Chromatography, Gel; D006801:Humans; D007768:Lactalbumin / Q000378:metabolism*; D008081:Liposomes; D008274:Magnesium / Q000494:pharmacology*; D009124:Muscle Proteins / Q000378:metabolism*; D010320:Parvalbumins / Q000378:metabolism*; D011489:Protein Denaturation; D012487:Salmonidae; D013050:Spectrometry, Fluorescence
chemical_list: D002135:Calcium-Binding Proteins; D008081:Liposomes; D009124:Muscle Proteins; D010320:Parvalbumins; D015060:1,2-Dipalmitoylphosphatidylcholine; D007768:Lactalbumin; D008274:Magnesium; D002118:Calcium
vernacular_title: Vliianie ionov kal'tsiia i magniia na vzaimodeĭstvie kal'tsiĭsviazyvaiushchikh belkov parval'bumina i alpha-laktal'bumina s vezikulami dipal'mitoilfosfatidilkholina.