title: Adrenergic and serotoninergic mechanisms in human hand arteries and veins studied by fluorescence histochemistry and in vitro pharmacology.
abstract: Isolated hand arteries and veins from healthy human subjects were tested in vitro for their contractile response to adrenergic agonists and 5-hydroxytryptamine (5-HT) under standardized conditions. This allowed for quantitative estimation of various receptor characteristics. The relative sympathomimetic potency suggested alpha-adrenergic receptors, which was confirmed in Schild plots following phentolamine antagonism of the response (pA2 for artery 7.57, for vein 7.75). 5-HT contracted with a relative potency approximately equal to noradrenaline and adrenaline in arteries, but only one fifth to one tenth of the catecholamine activity in veins. Ketanserin inhibited the 5-HT response in a competitive, probably also irreversible, manner in arteries (pA2 9.50, KA 8.90 X 10(-7) M). In the veins, ketanserin counteracted the 5-HT-induced contraction in a noncompetitive way.
authors.forename: B
authors.initials: B
authors.lastname: Arneklo-Nobin
authors.forename: C
authors.initials: C
authors.lastname: Owman
mesh_terms: D001158:Arteries / Q000032:analysis / Q000294:innervation*; D001667:Binding, Competitive; D001679:Biogenic Amines / Q000032:analysis; D004837:Epinephrine / Q000494:pharmacology; D005260:Female; D006225:Hand / Q000098:blood supply*; D006801:Humans; D007545:Isoproterenol / Q000494:pharmacology; D007650:Ketanserin; D008297:Male; D008856:Microscopy, Fluorescence; D009119:Muscle Contraction / Q000187:drug effects; D009638:Norepinephrine / Q000032:analysis; D010646:Phentolamine / Q000494:pharmacology; D010880:Piperidines / Q000494:pharmacology; D011941:Receptors, Adrenergic / Q000378:metabolism; D012701:Serotonin / Q000494:pharmacology / Q000502:physiology*; D013564:Sympathetic Nervous System / Q000502:physiology*; D014680:Veins / Q000032:analysis / Q000294:innervation*
chemical_list: D001679:Biogenic Amines; D010880:Piperidines; D011941:Receptors, Adrenergic; D012701:Serotonin; D007650:Ketanserin; D007545:Isoproterenol; D009638:Norepinephrine; D004837:Epinephrine; D010646:Phentolamine