title: Analysis of a TH1----TH2 helper cell circuit.
abstract: In the present study, the T15 idiotype-recognizing T helper cell circuit was dissected with respect to its homeostasis, interactive specificity, stability over time, and effects on B cell expression. Analysis of the TH1 cells by adoptive transfer experiments indicates their short-lived state of activity, during which TH2 cells are stimulated. TH1 cell activity was also directly monitored by the use of TNP-anti-T15 hybridoma antigens. It was found that TH1 cells are detected 1 wk after priming with PC-Hy, whereas TH2 cells become activated after 4 wk of priming. Comparative analysis of TH1 cells by using two different TNP-anti-T15 hybridoma antigens indicates a TH1 specificity for a shared idiotope. The stability over time of the TH1----TH2 circuit was demonstrated by comparing TH2 frequencies in young and old mice. Finally, we addressed the question of the function of the idiotype-recognizing T helper cells and showed that stimulation of T15-idiotype-specific TH2 cells can be correlated with a significant increase in the percentage of T15 idiotype in an anti-PC response. Collectively, these data describe an idiotype-specific T helper circuit as part of the network homeostasis of the immune system.
authors.forename: M
authors.initials: M
authors.lastname: McNamara
authors.forename: C Y
authors.initials: CY
authors.lastname: Kang
authors.forename: H
authors.initials: H
authors.lastname: Kohler
mesh_terms: D000367:Age Factors; D000818:Animals; D000917:Antibody Formation; D001402:B-Lymphocytes / Q000276:immunology*; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D007116:Immunization, Passive; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D007156:Immunologic Memory; D008211:Lymphocyte Cooperation; D008297:Male; D051379:Mice; D010767:Phosphorylcholine / Q000276:immunology; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*; D013997:Time Factors; D014301:Trinitrobenzenes / Q000276:immunology
chemical_list: D007130:Immunoglobulin Idiotypes; D014301:Trinitrobenzenes; D010767:Phosphorylcholine