[Thymus-dependent suppression of erythroid colony formation induced by concanavalin A in the cloning of pluripotent hematopoietic stem cells of the spleen].
The injection of the polyclonal activator of T-cells--concanavalin A--to normal mice is followed by a considerable decrease both in the erythroid colony-formation at the cloning of spleen CFUs, and in the number of T-cells and their helper activity in the same spleen suspensions. The suppression of erythroid colony-formation may be passively transferred by using spleen cells from mice treated with concanavalin A and irradiated lethally. Thymectomy of adult mice prevents from suppression of erythroid colony-formation induced by concanavalin A. These results suggest that the T-cells may suppress erythroid differentiation of hemopoietic stem cells.
Popov
B V
BV
Barbashina
V V
VV
D000818:Animals; D002999:Clone Cells / Q000187:drug effects / Q000528:radiation effects; D003114:Colony-Forming Units Assay; D003208:Concanavalin A / Q000494:pharmacology*; D004912:Erythrocytes / Q000187:drug effects* / Q000528:radiation effects; D004920:Erythropoiesis / Q000187:drug effects* / Q000528:radiation effects; D006412:Hematopoietic Stem Cells / Q000187:drug effects* / Q000528:radiation effects; D007108:Immune Tolerance / Q000187:drug effects* / Q000528:radiation effects; D007116:Immunization, Passive; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D013154:Spleen / Q000166:cytology / Q000187:drug effects* / Q000528:radiation effects; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology* / Q000528:radiation effects; D006377:T-Lymphocytes, Helper-Inducer / Q000187:drug effects / Q000276:immunology / Q000528:radiation effects; D013934:Thymectomy
D003208:Concanavalin A
Timuszavisimaia supressiia éritroidnogo kolonieobrazovaniia, indutsirovannaia konkanavalinom A, pri klonirovanii polipotentnykh stvolovykh krovetvornykh kletok selezenki.