A whole-cell patch clamp technique which minimizes cell dialysis.
A variant of the whole-cell patch clamp technique is described which allows measurement of whole-cell ionic currents in small cells while minimizing cell dialysis with the pipette solution. The technique involves the application of negative pressure to the inside of small (less than 1 micron) tip diameter pipettes placed on the cell surface to achieve high resistance seals and membrane rupture. The technique has been used successfully in a variety of different types of cells to study membrane currents carried by Ca and K, currents generated by exchange carriers as well as electrical coupling between cells. Overall, the technique seems well suited for the study of ionic currents in small cells, and provides an alternative to conventional patch clamping techniques which necessitate intracellular dialysis.
Hume
J R
JR
Department of Physiology, University of Nevada School of Medicine, Reno 89557-0046.
Leblanc
R N
RN
D000200:Action Potentials; D000818:Animals; D016275:Atrial Function; D002118:Calcium / Q000502:physiology*; D004558:Electric Stimulation; D004594:Electrophysiology / Q000295:instrumentation* / Q000379:methods; D006168:Guinea Pigs; D006325:Heart Atria / Q000166:cytology; D006352:Heart Ventricles / Q000166:cytology; D006801:Humans; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000502:physiology*; D011188:Potassium / Q000502:physiology*; D011817:Rabbits; D011898:Ranidae; D016276:Ventricular Function
D011188:Potassium; D002118:Calcium