[Comparative study of physico-chemical and biological properties of human somatotropin produced by genetic engineering and isolated from the pituitary gland].
Human somatotropin hormono (STH), produced by means of gene engineering in the complex program "Human growth hormone", managed by the Academy of Sciences of the USSR, Ministry of Medical and Biological Industry of the USSR and Ministry of Public Health of the USSR, was shown to be similar in its physico-chemical properties to the main isoform of highly purified STH, isolated from human hypophysis. As distinct from the hypophyseal STH (STHhyp) containing minor isoforms of the hormone, the preparation of biosynthetic STH (des-Phe1-STH; STHbio) proved to be homogeneous. Studies of biological properties showed that STHbio exhibited high, similar to STHhyp, immunological, growth-stimulating and insulin-like activities as well as it possessed the lipotropic effect in vivo. The lipotropic effect of STHbio in vivo was less prolonged as compared with that of STHhyp, while in vitro it was only slightly expressed in isolated rabbit fat tissue. The effect did not depend on the hormone dose, apparently due to either absence of the hormone modified forms in the STHbio preparation or other hypophyseal contaminating substances responsible for the lipotropic activity. STHbio, similarly to STHhyp, did not stimulate DNA synthesis in blood serum-free culture of human fibroblasts. Studies of STHbio biological properties suggest that multifunctionality of native STHhyp appear to depend on intrinsic specificity of its molecule.
Elizarova
G P
GP
Keda
Iu M
IuM
Kiseleva
A G
AG
Osipova
T A
TA
Bulatov
A A
AA
D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000096:biosynthesis; D004591:Electrophoresis, Polyacrylamide Gel; D004838:Epiphyses / Q000254:growth & development; D005818:Genetic Engineering; D005947:Glucose / Q000378:metabolism; D013006:Growth Hormone / Q000032:analysis* / Q000096:biosynthesis / Q000494:pharmacology; D006801:Humans; D008066:Lipolysis / Q000187:drug effects; D010902:Pituitary Gland / Q000032:analysis*; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains; D011994:Recombinant Proteins / Q000032:analysis / Q000096:biosynthesis / Q000494:pharmacology
D000596:Amino Acids; D011994:Recombinant Proteins; D013006:Growth Hormone; D004247:DNA; D005947:Glucose
Sravnitel'noe issledovanie fiziko-khimicheskikh i biologicheskikh svoĭstv somatotropina cheloveka, poluchennogo gennoinzhenernym metodom i vydelennogo iz gipofizov.