Orosomucoid (alpha-1 acid glycoprotein) phenotyping by use of immobilized pH gradients with 8 M urea and immunoblotting. A new variant encountered in a population study.
Orosomucoid (ORM) phenotyping has been performed on 329 unrelated Swiss subjects, using immobilized pH gradients with 8 M urea and 2% v/v 2-mercaptoethanol followed by immunoblotting. After desialylation the band patterns of ORM confirmed that the polymorphism of the structural locus ORM1 is controlled by three codominant autosomal alleles (ORM1*F1, ORM1*S and ORM1*F2). One rare and one new allele were detected. The rare variant, tentatively assigned to the second structural locus ORM2, is observed in a cathodal position and named ORM2 B1. The new variant, tentatively assigned to the first structural locus ORM1, is observed in a region located between ORM1 S and ORM1 F2, and named ORM1 F3. Moreover, the pI values of the ORM variants have been measured accurately with Immobiline Dry Plates (LKB): they were found to be within the pH range 4.93-5.14.
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Clinique Psychiatrique Universitaire de Lausanne, Hôpital de Cery, Switzerland.
Cuendet
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Baumann
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D005796:Genes; D005799:Genes, Dominant; D006801:Humans; D006863:Hydrogen-Ion Concentration; D015151:Immunoblotting; D009961:Orosomucoid / Q000235:genetics*; D010641:Phenotype; D014508:Urea
D009961:Orosomucoid; D014508:Urea