Lysosomal enzymes in plasma, liver and spleen from rats with carbon tetrachloride-induced liver cirrhosis.
Plasma levels of the lysosomal enzymes, beta-hexosaminidase and beta-glucuronidase, were analyzed in rats with carbon tetrachloride induced liver cirrhosis. Out of 22 animals, 15 showed cirrhotic and 4 pre-cirrhotic livers. 4 cirrhotic animals exhibited cholestatic features with increased bilirubin, alkaline phosphatase (ALP) and aspartate aminotransferase (ASAT) in plasma. The remaining 15 pre-cirrhotic and cirrhotic rats showed clear significant changes only in ASAT levels. These 15 rats showed no consistent increase in plasma, spleen or liver lysosomal enzyme activities, whereas the 4 rats with cholestatic features exhibited considerable increases of lysosomal enzymes. The increased activities might be attributed to decreased biliary excretion and/or increased production of lysosomal enzymes by activated macrophages.
Melén
K
K
Hultberg
B
B
Hägerstrand
I
I
Isaksson
A
A
Joelsson
B
B
Bengmark
S
S
D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D001219:Aspartate Aminotransferases / Q000032:analysis; D001663:Bilirubin / Q000097:blood; D002251:Carbon Tetrachloride / Q000633:toxicity*; D005966:Glucuronidase / Q000032:analysis; D006596:Hexosaminidases / Q000032:analysis; D008099:Liver / Q000201:enzymology*; D008103:Liver Cirrhosis / Q000097:blood / Q000139:chemically induced* / Q000201:enzymology; D008247:Lysosomes / Q000201:enzymology*; D008264:Macrophages / Q000201:enzymology; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013154:Spleen / Q000201:enzymology*; D001619:beta-N-Acetylhexosaminidases
D002251:Carbon Tetrachloride; D001219:Aspartate Aminotransferases; D000469:Alkaline Phosphatase; D006596:Hexosaminidases; D005966:Glucuronidase; D001619:beta-N-Acetylhexosaminidases; D001663:Bilirubin