title: Excised human neoplastic tissues are more sensitive to heat than the adjacent normal tissues.
abstract: In tissues from patients subjected to gastrectomy or colectomy, the heat sensitivity was determined in the case of 23 neoplastic, 15 gastric, 8 colorectal, and the adjacent normal tissues, using the in vitro succinate dehydrogenase inhibition test. The succinate dehydrogenase (SD) activity of tissue fragments was assayed, following exposure to heat at 43 degrees C (heat treatment) or 37 degrees C (control) for 5, 10, 15 or 20 h. The sensitivity to heat treatment was estimated by the percentage of SD activity of the heat-treated cells, compared to that of control cells. The decrease in SD activity varied in the tumor tissue, following exposure to heat. The SD activity decreased to a greater extent in the tumor tissue than in the adjacent normal tissue, in each case. The mean +/- standard deviation of SD activity, following exposure to heat treatment for 20 h, was 32.1 +/- 14.0% for the tumour tissues and 52.4 +/- 10.4% for the adjacent normal tissues, with a statistically significant difference (p less than 0.01). These results show that the assay of heat sensitivity is meaningful for prediciting the effectiveness of hyperthermia and that hyperthermia has a selectivity for treating a malignant lesion.
authors.affiliation: Cancer Center of Kyushu University Hospital, Faculty of Medicine, Fukuoka, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Maehara
authors.forename: T
authors.initials: T
authors.lastname: Kusumoto
authors.forename: H
authors.initials: H
authors.lastname: Kusumoto
authors.forename: H
authors.initials: H
authors.lastname: Anai
authors.forename: K
authors.initials: K
authors.lastname: Akazawa
authors.forename: K
authors.initials: K
authors.lastname: Sugimachi
mesh_terms: D000328:Adult; D000368:Aged; D002470:Cell Survival; D015179:Colorectal Neoplasms / Q000473:pathology / Q000503:physiopathology*; D005260:Female; D006358:Hot Temperature; D006801:Humans; D006979:Hyperthermia, Induced; D008297:Male; D008875:Middle Aged; D013274:Stomach Neoplasms / Q000473:pathology / Q000503:physiopathology*