Two cytoplasmically inherited oligomycin-resistant Chinese hamster cell lines exhibit an altered mitochondrial translation product.
Mitochondria from two different cytoplasmically inherited oligomycin-resistant Chinese hamster ovary cell lines synthesize an altered polypeptide compared to mitochondria from wild-type cells. For example, mitochondria from both oligomycin-resistant cell lines synthesize a polypeptide with a molecular weight of approximately 20,500, which is present in very low amounts in wild-type cells. In contrast, mitochondria from wild-type cells synthesize a polypeptide with a molecular weight of approximately 19,500, which is present in very low amounts in one of the oligomycin-resistant mutants and in reduced amounts in the other mutant. The gene which encodes this altered polypeptide is cytoplasmically transferred together with the oligomycin-resistant phenotype. This is the first example in mammalian cells where an altered mitochondrial gene product is shown to be associated with the cytoplasmic transfer of oligomycin resistance.
Shew
J Y
JY
Breen
G A
GA
D000251:Adenosine Triphosphatases / Q000235:genetics; D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D004351:Drug Resistance; D008928:Mitochondria / Q000378:metabolism*; D008970:Molecular Weight; D009154:Mutation; D009840:Oligomycins / Q000494:pharmacology*; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics*
D009840:Oligomycins; D011506:Proteins; D000251:Adenosine Triphosphatases