Effects of multiple freeze thaws and various temperatures on the reactivity of human immunodeficiency virus antibody using three detection assays.
The effects of multiple freeze-thaw cycles and various temperatures (-20 degrees C, 4 degrees C, 25 degrees C, 37 degrees C) on the reactivity of human immunodeficiency virus (HIV) antibodies were evaluated using current ELISA, recombinant, and Western blot methodologies. Twenty consecutive freeze-thaw cycles and storage of specimens at -20 degrees C and 4 degrees C for 57 days resulted in no loss of HIV antibody reactivity nor false positive samples. Maintenance of clinical specimens at 25 degrees C and 37 degrees C for 57 days resulted in some loss of HIV antibody reactivity, but all positive and weakly reacting samples remained positive, and negative samples were unaffected.
Fipps
D R
DR
Department of Virus Disease, Walter Reed Army Institute of Research, Walter Reed Army Medical Center, Washington, DC 20307-5100.
Damato
J J
JJ
Brandt
B
B
Burke
D S
DS
D000914:Antibodies, Viral / Q000032:analysis*; D004355:Drug Stability; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005615:Freezing; D006678:HIV / Q000276:immunology*; D015483:HIV Antibodies; D006801:Humans; D007159:Immunologic Tests / Q000379:methods; D013696:Temperature
D000914:Antibodies, Viral; D015483:HIV Antibodies