title: Detection in human platelets of phospholipase A2 activity which preferentially hydrolyzes an arachidonoyl residue.
abstract: It has been generally considered that highly specific liberation of arachidonic acid is induced upon the stimulation of the platelets, although the molecular mechanism of the regulation of its action has not been well understood. An aim of the present study is to clarify the role of phospholipase A2 in the arachidonic acid metabolism within human platelets. Phosphatidylcholine or phosphatidylethanolamine with arachidonate at the sn-2 position of glycerol was cleaved efficiently by phospholipase A2 activity in homogenates as well as in the cytoplasmic fraction of human platelets, leading to the selective liberation of free arachidonate, whereas phospholipids with linoleate were hardly hydrolyzed under the same conditions. Double-reciprocal plots of kinetic data further strengthened the conclusion that human platelet phospholipase A2 showed high selectivity for arachidonoyl residue. This enzyme may play a crucial role in the intracellular metabolism of the arachidonate of phospholipids.
authors.affiliation: Faculty of Pharmaceutical Sciences, University of Tokyo.
authors.forename: D K
authors.initials: DK
authors.lastname: Kim
authors.forename: I
authors.initials: I
authors.lastname: Kudo
authors.forename: K
authors.initials: K
authors.lastname: Inoue
mesh_terms: D016718:Arachidonic Acid; D001095:Arachidonic Acids; D001792:Blood Platelets / Q000201:enzymology*; D006801:Humans; D007700:Kinetics; D010740:Phospholipases / Q000097:blood*; D010741:Phospholipases A / Q000097:blood*; D054467:Phospholipases A2
chemical_list: D001095:Arachidonic Acids; D016718:Arachidonic Acid; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2