Liquid-chromatographic determination of ciprofloxacin in serum and urine.
We describe the liquid-chromatographic determination of ciprofloxacin in patients' serum and urine. Serum samples were prepared by precipitating protein with perchloric acid. Urine samples were diluted 100-fold with mobile phase. The mobile phase, consisting of pH 3 phosphate buffer/acetonitrile/methanol (81/5/14, by vol), was pumped through a mu Bondapak C18 reversed-phase column at 1.5 mL/min. Fluorescence of the effluent was monitored, at wavelengths for excitation and emission of 270 and 440 nm, respectively. Standard curves were linearly related to concentration from 0.08 to 10 mg/L for serum, 1 to 20 mg/L for urine. The procedure was evaluated in a clinical setting to determine its usefulness in studying the pharmacokinetics of ciprofloxacin in patients with concurrent diseases and receiving multiple drug therapies.
Nix
D E
DE
De Vito
J M
JM
Schentag
J J
JJ
D002853:Chromatography, Liquid; D002939:Ciprofloxacin; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007700:Kinetics; D011804:Quinolines / Q000097:blood / Q000302:isolation & purification* / Q000652:urine
D011804:Quinolines; D002939:Ciprofloxacin