Laser-induced multiphoton fluorescence of hemoglobin.
Hemoglobin (Hb) and other samples were irradiated by the 1064 nm nanosecond pulses of an Nd:YAG laser. At room temperature, we observed two red fluorescence bands, which resulted from the absorption of two 1064 nm photons in Hb, and measured the fluorescence emission spectra in the red spectral region for Hb and hematoporphyrin derivative. A red shift of the wavelength and a mirror image in the intensity of fluorescence emissions by two-photon excitation were observed. It is shown that the fluorescence of Hb originates from the heme group. In addition, we also observed fluorescence emission at 392 nm for Hb, which may be from its tryptophan groups.
Zhang
J R
JR
Laboratory of Laser Spectroscopy, Anhui Institute of Optics and Fine Mechanics, Academia Sinica, China.
Xu
Y W
YW
Deng
Y M
YM
Wu
C K
CK
Jiang
S P
SP
Lian
S H
SH
D005453:Fluorescence; D006454:Hemoglobins / Q000528:radiation effects*; D007834:Lasers; D010777:Photochemistry
D006454:Hemoglobins