Peritoneal transport of beta-lactam antibiotics: effects of plasma protein binding and the interspecies relationship.
In order to examine quantitatively the effect of plasma protein binding on the peritoneal transport of beta-lactam antibiotics, we employed a kinetic model based on the pore theory of transcapillary exchange. This model incorporates the changes in the volume, osmolality, and antibiotic concentration in the dialysate, so that the apparent capillary membrane permeability (Pd) and the reflection coefficient (sigma d) of an antibiotic could be assessed. Six cephalosporins (cefatrizine, cefazolin, cefpiramide, ceftazidime, ceftriaxone, cephaloridine) were used as model compounds. While the unbound fractions of these antibiotics ranged widely from 0.08 to 0.57, including linear and nonlinear protein binding, the concentration-time profiles in plasma and the peritoneal dialysate after intravenous administration in rats could be interpreted well by our model, assuming that only the unbound antibiotic is available for the peritoneal transport. The estimated Pd values were almost the same among the drugs examined. Moreover, the Pd values of cefazolin in mice, rats, and rabbits exhibited a 0.83-power dependency on the animal body weight, indicating that Pd is significantly related to the peritoneal surface area. On the other hand, the sigma d values of cefazolin were found to be almost the same among the animal species examined. Finally, the concentration-time profile of cefazolin in the dialysate after intravenous administration in a patient with end-stage renal failure was successfully predicted using the Pd value extrapolated from those of the experimental animals.
Deguchi
Y
Y
Hospital Pharmacy, Faculty of Pharmaceutical Sciences, Kanazawa, Japan.
Nakashima
E
E
Ishikawa
F
F
Sato
H
H
Tamai
I
I
Matsushita
R
R
Tofuku
Y
Y
Ichimura
F
F
Tsuji
A
A
D000368:Aged; D000818:Animals; D000900:Anti-Bacterial Agents / Q000097:blood / Q000378:metabolism*; D001798:Blood Proteins / Q000378:metabolism*; D003956:Dialysis; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009994:Osmolar Concentration; D010537:Peritoneum / Q000378:metabolism; D011485:Protein Binding; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains; D013045:Species Specificity; D047090:beta-Lactams
D000900:Anti-Bacterial Agents; D001798:Blood Proteins; D047090:beta-Lactams