title: Photoprotective effect of vitamins A and E on polyamine and oxygenated free radical metabolism in hairless mouse epidermis.
abstract: The purpose of this study was to confirm the photoprotective effect on skin of vitamins A and E, due to inhibition of polyamine synthesis and production of free radicals. These variables were measured in the lumbar epidermis of the female hairless mouse subjected to UVA + B irradiation. Polyamines were assayed in epidermal homogenate by HPLC, and production of oxygenated free radicals was determined by spectrofluorometric assay of malonyl dialdehyde. It was determined that butyl-hydroxy-toluene and vitamin E inhibited production of free radicals (56% and 60%, respectively) and caused a significant reduction in polyamine biosynthesis (P less than 0.01), whereas the inhibitory effect of malonyl dialdehyde induced by vitamin A (30%) had no associated effect on polyamine metabolism.
authors.affiliation: Laboratoire de Biochimie et de Physiologie, Faculté de Pharmacie, Nantes, France.
authors.forename: N
authors.initials: N
authors.lastname: Khettab
authors.forename: M C
authors.initials: MC
authors.lastname: Amory
authors.forename: G
authors.initials: G
authors.lastname: Briand
authors.forename: B
authors.initials: B
authors.lastname: Bousquet
authors.forename: A
authors.initials: A
authors.lastname: Combre
authors.forename: P
authors.initials: P
authors.lastname: Forlot
authors.forename: M
authors.initials: M
authors.lastname: Barey
mesh_terms: D000818:Animals; D002084:Butylated Hydroxytoluene / Q000008:administration & dosage / Q000494:pharmacology; D002338:Carotenoids / Q000008:administration & dosage / Q000494:pharmacology; D004817:Epidermis / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D005260:Female; D005609:Free Radicals; D006801:Humans; D008315:Malondialdehyde / Q000096:biosynthesis; D051379:Mice; D008812:Mice, Hairless; D010100:Oxygen / Q000378:metabolism*; D011073:Polyamines / Q000096:biosynthesis*; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D014466:Ultraviolet Rays; D014801:Vitamin A / Q000494:pharmacology*; D014810:Vitamin E / Q000494:pharmacology*; D019207:beta Carotene
chemical_list: D005609:Free Radicals; D011073:Polyamines; D011837:Radiation-Protective Agents; D019207:beta Carotene; D014801:Vitamin A; D014810:Vitamin E; D002084:Butylated Hydroxytoluene; D002338:Carotenoids; D008315:Malondialdehyde; D010100:Oxygen