Human nasal septal cartilage: local distribution of different enzyme activities in healthy adults and acromegalic patients.
Septal cartilage was obtained during septoplasty from healthy adults or during transnasal hypophysectomy from acromegalic patients. Small strips of cartilage were excised from five different areas of the septum: the anterior free end, the suprapremaxillary area, the central area, the posterior area, and the caudal prolongation of the septum. Five different enzymatic pathways were analyzed in these areas. Cathepsin D, an acid proteinase, did not show a specific local activity pattern and was not influenced by the augmented growth hormone level in acromegaly, whereas cathepsin B, a neutral proteinase, showed its highest activity in the caudal prolongation and the posterior area and was significantly increased in all areas in acromegaly. Beta-hexosaminidase activity was highest in the central and posterior area and caudal prolongation of the septum. In acromegaly, a significant increase of its activity was found in the suprapremaxillary and posterior area. Acid phosphatase activity was highest in the caudal prolongation of the septum, but its activity was significantly increased in all tested areas in acromegaly. Alkaline phosphatase activity could only be found in the posterior area and the caudal prolongation in healthy adults. However, in acromegaly this enzyme could be detected in the central area and the posterior end of the suprapremaxillary area suggesting an altered process of mineralization. A distinct local pattern of enzymes related to intercellular substance metabolism and mineralization can be demonstrated in the septal cartilage of healthy adults and acromegalic patients.
Vetter
U
U
Helbing
G
G
Pirsig
W
W
Heinze
E
E
Gammert
C
C
Landolt
A
A
D000135:Acid Phosphatase / Q000032:analysis; D000172:Acromegaly / Q000201:enzymology*; D000328:Adult; D000469:Alkaline Phosphatase / Q000032:analysis; D002356:Cartilage / Q000201:enzymology*; D002401:Cathepsin B; D002402:Cathepsin D / Q000032:analysis; D002403:Cathepsins / Q000032:analysis; D005260:Female; D006596:Hexosaminidases / Q000032:analysis; D006801:Humans; D008297:Male; D008875:Middle Aged; D009300:Nasal Septum / Q000033:anatomy & histology / Q000201:enzymology*; D001619:beta-N-Acetylhexosaminidases
D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D006596:Hexosaminidases; D001619:beta-N-Acetylhexosaminidases; D002403:Cathepsins; D002401:Cathepsin B; D002402:Cathepsin D