The chemiluminescent response of human monocytes to red cells sensitized with monoclonal anti-Rh(D) antibodies.
Luminol-enhanced chemiluminescence (CL) was used to assess the metabolic response of human monocytes to red cells sensitized with known amounts of anti-Rh(D). Monoclonal antibodies were used to facilitate a comparison between the functional activities of IgG1 and IgG3 subclasses. The detection of CL provided a simple, rapid and semi-quantitative means of measuring monocyte response to sensitized red cells (IgG-RBC). Monocyte response to IgG3-RBC was quantitatively greater, more rapid and less susceptible to inhibition by fluid phase IgG than monocyte response to IgG1-RBC. The minimum levels of sensitization required to elicit CL from monocytes were approximately 2500 IgG3 molecules per red cell, or approximately 5000 IgG1 molecules per cell.
Hadley
A G
AG
Blood Group Reference Laboratory, Radcliffe Infirmary, Oxford.
Kumpel
B M
BM
Merry
A H
AH
D000911:Antibodies, Monoclonal / Q000276:immunology*; D004912:Erythrocytes / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D008163:Luminescent Measurements; D008165:Luminol; D009000:Monocytes / Q000276:immunology*; D010587:Phagocytosis; D012204:Rh-Hr Blood-Group System / Q000276:immunology*; D012397:Rosette Formation
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D012204:Rh-Hr Blood-Group System; D008165:Luminol