Functional activity of rat testicular cells in culture.
Testicular cells from adult hypophysectomized rats were cultured for 10 or 12 days, and the effect of treatment with hCG (10 ng/ml) on testosterone and progesterone production and the activity of the Leydig cell enzyme, 3 beta-hydroxysteroid dehydrogenase, were studied. Regardless of hormone treatment, on 4th day in culture a decline in the steroidogenic activity of cultured cells could be observed. Treatment with hCG resulted in stimulation of steroidogenesis on days 6 to 10 in culture, as measured by testosterone and progesterone production. Hormone treatment stimulated or inhibited the enzyme activity depending on the presence or absence in the culture medium of 10(-6) M spironolactone, an inhibitor of 17 alpha-hydroxylase, or an anti-androgen, cyproterone acetate.
Centol
I
I
Departamento de Bioquimica y Biologia Molecular. Universidad de La Laguna, Las Palmas de Gran Canaria, Spain.
Deniz
A
A
Benitez
L
L
Marrero
I
I
Fanjul
L F
LF
Ruiz de Galarreta
C M
CM
D015096:3-Hydroxysteroid Dehydrogenases / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D006063:Chorionic Gonadotropin / Q000494:pharmacology*; D007016:Hypophysectomy; D066298:In Vitro Techniques; D007985:Leydig Cells / Q000201:enzymology; D008297:Male; D011374:Progesterone / Q000096:biosynthesis*; D051381:Rats; D011919:Rats, Inbred Strains; D013148:Spironolactone / Q000494:pharmacology; D013737:Testis / Q000187:drug effects / Q000378:metabolism*; D013739:Testosterone / Q000096:biosynthesis*
D006063:Chorionic Gonadotropin; D013148:Spironolactone; D013739:Testosterone; D011374:Progesterone; D015096:3-Hydroxysteroid Dehydrogenases