Detection of O4-ethylthymine in human liver DNA.
O4-Ethyl-2'-deoxythymidine (O4-EtdThy) in human liver DNA was quantified, in order to monitor possible human exposure to ethylating agents, using a highly sensitive immunological detection method. In 30 of 33 cases analysed, O4-EtdThy was detected at above the detection limit (i.e., 3 x 10(-8) O4-EtdThy/2'-deoxy-thymidine[dThy]), indicating actual chronic exposure to ethylating agents. The mean content of O4-EtdThy in 19 cases of malignant tumours was significantly higher than that in 11 nonmalignant cases (p less than 0.05).
Huh
N
N
Department of Cancer Cell Research, University of Tokyo, Japan.
Satoh
M S
MS
Shiga
J
J
Kuroki
T
T
D004247:DNA / Q000032:analysis*; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000032:analysis; D004784:Environmental Monitoring; D006801:Humans; D008099:Liver / Q000032:analysis*; D008113:Liver Neoplasms / Q000032:analysis; D011863:Radioimmunoassay; D013941:Thymine / Q000031:analogs & derivatives* / Q000032:analysis
C040384:4-ethylthymine; C018534:O(6)-ethyl-2'-deoxyguanosine; D004247:DNA; D003849:Deoxyguanosine; D013941:Thymine