Immunological and endocrinological response to growth hormone therapy in short children.
We investigated the influence of human growth hormone (hGH) on mitogen-stimulated lymphoproliferation, in vitro IgM production, serum levels of immunoglobulins, somatomedin-C (Sm-C) values and serum growth-promoting activity (Thymidine Activity, TA) in 18 short children, aged between 6.6-14.5 years, undergoing a 3-month course of hGH therapy. Blood was collected the day before treatment (Group A), on the 5th day after patients were administered hGH daily (0.1 U/kg) i.m. for 4 days (Group B), after a 3-month course of hGH injected three times weekly, and finally before (Group C) and 24 h after an extra injection (Group D). In vitro IgM production from the patients' unstimulated lymphocytes decreased from 277 +/- 41 (Group A) to 168 +/- 38 (Group B), to 119 +/- 43 (Group C) and then to 119 +/- 28 ng/ml (Group D) (p less than 0.05). Using PWM-stimulated lymphocytes in vitro IgM production decreased from 2,015 +/- 464 (Group A) to 1,116 +/- 316 (Group B), then to 511 +/- 170 (Group C) and 968 +/- 295 ng/ml (Group D) (p less than 0.02). The variation of this decrease could be correlated with the variation of growth velocity during treatment (r = 0.619, p less than 0.05). In contrast, no significant changes were found following therapy either in serum levels of IgA, IgE, IgG, IgM, Sm-C and TA, or in phytohemagglutinin, concanavalin A and pokeweed mitogen-stimulated lymphoproliferation. Our data suggest that there is some relationship between growth hormone, growth and immunity.
Bozzola
M
M
Department of Paediatrics, University of Pavia, Italy.
Maccario
R
R
Cisternino
M
M
de Amici
M
M
Valtorta
A
A
Moretta
A
A
Biscaldi
I
I
Schimpff
R M
RM
D000293:Adolescent; D002648:Child; D005260:Female; D006128:Growth / Q000187:drug effects*; D013006:Growth Hormone / Q000008:administration & dosage*; D006801:Humans; D007070:Immunoglobulin A / Q000032:analysis; D007073:Immunoglobulin E / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000096:biosynthesis; D007334:Insulin-Like Growth Factor I / Q000097:blood; D008213:Lymphocyte Activation / Q000187:drug effects; D008297:Male; D013936:Thymidine / Q000097:blood
D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007073:Immunoglobulin E; D007334:Insulin-Like Growth Factor I; D013006:Growth Hormone; D013936:Thymidine