title: The complete amino acid sequence of bovine milk angiogenin.
abstract: The amino acid sequence of angiogenin isolated from bovine milk was deduced by gas-phase sequencing of the protein and its fragments. The protein contains 125 residues and has a calculated molecular mass of 14,577 Da. The sequence is highly homologous (65% identity) to the sequence of human angiogenin, most of the differences being the result of conservative replacements. Like human angiogenin, the bovine protein is also homologous to bovine pancreatic RNase A (34% identity) and the three major active site residues known to be involved in the catalytic process, His-14, Lys-41 and His-115, are conserved. When tested against conventional substrates for RNase A activity, bovine angiogenin displays the same selective ribonucleolytic activity as human angiogenin. The sequence of bovine angiogenin contains the cell recognition tripeptide Arg-Gly-Asp which is not present in the human protein.
authors.affiliation: Service de Chimie des Biomolécules, Institut Pasteur de Lille, France.
authors.forename: P
authors.initials: P
authors.lastname: Maes
authors.forename: D
authors.initials: D
authors.lastname: Damart
authors.forename: C
authors.initials: C
authors.lastname: Rommens
authors.forename: J
authors.initials: J
authors.lastname: Montreuil
authors.forename: G
authors.initials: G
authors.lastname: Spik
authors.forename: A
authors.initials: A
authors.lastname: Tartar
mesh_terms: D000595:Amino Acid Sequence; D043925:Angiogenesis Inducing Agents; D000818:Animals; D002417:Cattle; D005260:Female; D006133:Growth Substances; D006801:Humans; D008892:Milk; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism; D010446:Peptide Fragments / Q000302:isolation & purification; D012259:Ribonuclease, Pancreatic; D012260:Ribonucleases / Q000378:metabolism; D013045:Species Specificity
chemical_list: D043925:Angiogenesis Inducing Agents; D006133:Growth Substances; D009363:Neoplasm Proteins; D010446:Peptide Fragments; D012260:Ribonucleases; C046366:angiogenin; D012259:Ribonuclease, Pancreatic