title: Loss of low-affinity serotonin receptors upon storage of human platelets.
abstract: Platelets actively accumulate virtually all plasma serotonin within their dense granules. As a readily isolated, homogeneous cell type, platelets have served as a model for serotonin uptake into neurological tissue, in addition to defining the role of serotonin in hemostasis. The number of serotonin receptor types on the platelet membrane and the function of these receptors has not been conclusively demonstrated. The presence of different receptor types that may be altered or lost in disease or upon aging (in vitro storage or in vivo) could have significant physiological effects on platelet function. This report demonstrates that at least two receptor types are present on freshly prepared human platelets. However, after 3 to 4 days of storage in autologous plasma, the low-affinity, high-capacity serotonin receptor appears to be lost. This phenomenon probably accounts for some of the discrepancies reported in the literature. The high-affinity receptor present in both freshly isolated and stored platelets binds about 9 x 10(3) serotonin molecules per platelet. Binding can be completely blocked by imipramine; however, some passive diffusion appears to occur even at the low level of extracellular serotonin concentrations employed in these studies (nanomolar range). The influx of serotonin into platelets appears to be poorly reversible, even in reserpine-treated cells, where the extravesicular cytoplasmic concentration would be high. The loss of the low-affinity serotonin receptor type reported in these studies may be directly or indirectly associated with the reduced responsiveness observed in stored platelets.
authors.affiliation: Department of Biological Chemistry, Hahnemann University School of Medicine, Philadelphia, Pennsylvania 19102.
authors.forename: G
authors.initials: G
authors.lastname: Soslau
authors.forename: J
authors.initials: J
authors.lastname: Gillen
authors.forename: J
authors.initials: J
authors.lastname: Parker
mesh_terms: D001692:Biological Transport / Q000187:drug effects; D001792:Blood Platelets / Q000032:analysis* / Q000166:cytology / Q000378:metabolism; D002470:Cell Survival; D002794:Choline / Q000494:pharmacology; D003594:Cytoplasmic Granules / Q000378:metabolism; D006801:Humans; D007099:Imipramine / Q000494:pharmacology; D007700:Kinetics; D011985:Receptors, Serotonin / Q000032:analysis* / Q000378:metabolism; D012110:Reserpine / Q000494:pharmacology; D012701:Serotonin / Q000378:metabolism
chemical_list: D011985:Receptors, Serotonin; D012701:Serotonin; D012110:Reserpine; D002794:Choline; D007099:Imipramine