title: An analysis of the components in UW solution using the isolated perfused rabbit liver.
abstract: The isolated perfused rabbit liver model has been used to determine the essential components of the UW solution for hepatic preservation by simple cold storage. Livers were stored on ice for 48 hr after initial flushing with the solution being tested, and then reperfused at 38 degrees C in an isolated perfusion circuit; bile flow and enzyme (SGOT, SGPT, and LDH) release during a 2-hr period were recorded. All solutions tested contained phosphate (25 mM) as a buffer and magnesium sulfate (5 mM). Sodium can be substituted for potassium without adverse effects. Lactobionate, raffinose and glutathione cannot be omitted; all other components can be eliminated without altering the effectiveness of the solution in this model.
authors.affiliation: Department of Surgery, University of Wisconsin, Madison 53792.
authors.forename: N V
authors.initials: NV
authors.lastname: Jamieson
authors.forename: S
authors.initials: S
authors.lastname: Lindell
authors.forename: R
authors.initials: R
authors.lastname: Sundberg
authors.forename: J H
authors.initials: JH
authors.lastname: Southard
authors.forename: F O
authors.initials: FO
authors.lastname: Belzer
mesh_terms: D000241:Adenosine; D000410:Alanine Transaminase / Q000097:blood; D000493:Allopurinol; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001646:Bile / Q000378:metabolism; D005978:Glutathione; D007328:Insulin; D008099:Liver; D009926:Organ Preservation; D019188:Organ Preservation Solutions; D010477:Perfusion; D011188:Potassium / Q000032:analysis; D011817:Rabbits; D011887:Raffinose; D012964:Sodium / Q000032:analysis; D012996:Solutions / Q000032:analysis*
chemical_list: D007328:Insulin; D019188:Organ Preservation Solutions; D012996:Solutions; C055206:University of Wisconsin-lactobionate solution; D000493:Allopurinol; D012964:Sodium; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose; D011188:Potassium