title: Identification of oversulphated galactosaminoglycans in intestinal-mucosal mast cells of rats infected with the nematode worm Nippostrongylus brasiliensis.
abstract: The oversulphated galactosaminoglycans synthesized by rat mucosal mast cells were isolated from the small intestine of animals infected with the nematode Nippostrongylus brasiliensis, which causes proliferation of these cells. The 35S-labelled polysaccharides were degraded by digestion with chondroitinase ABC, and the structures of the disaccharide products were determined by cleavage with mercuric acetate followed by electrophoretic characterization of the resultant sulphated monosaccharides. It was concluded that about half of the disulphated disaccharide units in the polysaccharide consisted of chondroitin sulphate E-type structures [GlcA-GalNAc(4,6-di-OSO3)], in which both sulphate groups were located on the N-acetylgalactosamine unit. The remainder consisted of isomeric structures with one sulphate group on the N-acetylgalactosamine residue and one on the hexuronic acid unit and presumably represented the dermatan sulphate-type sequence [IdoA(2-OSO3)-GalNAc(4-OSO3)].
authors.affiliation: Department of Veterinary Medical Chemistry, Swedish University of Agricultural Sciences, Uppsala.
authors.forename: M
authors.initials: M
authors.lastname: Kusche
authors.forename: U
authors.initials: U
authors.lastname: Lindahl
authors.forename: L
authors.initials: L
authors.lastname: Enerbäck
authors.forename: L
authors.initials: L
authors.lastname: Rodén
mesh_terms: D000818:Animals; D002852:Chromatography, Ion Exchange; D004187:Disaccharides / Q000302:isolation & purification; D004590:Electrophoresis, Paper; D007413:Intestinal Mucosa / Q000378:metabolism*; D008407:Mast Cells / Q000378:metabolism; D008628:Mercury; D009349:Nematode Infections / Q000378:metabolism*; D009559:Nippostrongylus; D011134:Polysaccharides / Q000378:metabolism*; D051381:Rats; D013429:Sulfatases; D013431:Sulfates / Q000032:analysis; D013462:Sulfur Radioisotopes; D013464:Sulfuric Acids / Q000378:metabolism
chemical_list: D004187:Disaccharides; D011134:Polysaccharides; D013431:Sulfates; D013462:Sulfur Radioisotopes; D013464:Sulfuric Acids; C007815:galactosaminoglycan; D013429:Sulfatases; C046341:chondro-6-sulfatase; D008628:Mercury; C029823:mercuric acetate