title: Selective and indirect modulation of human multipotential and erythroid hematopoietic progenitor cell proliferation by recombinant human activin and inhibin.
abstract: Activin and inhibin are biomolecules that, respectively, enhance and suppress the release of follicle-stimulating hormone from pituitary cells in vitro. Purified recombinant human (rhu) activin A and inhibin A were assessed for their effects on colony formation in vitro by human multipotential (CFU-GEMM), erythroid (BFU-E), and granulocyte-macrophage (CFU-GM) progenitor cells. It was found that (i) rhu-activin A enhances colony formation by normal bone marrow erythroid and multipotential progenitor cells; (ii) purified rhu-inhibin A decreases activin, but not rhu-interleukin 3, rhu-granulocyte-macrophage colony-stimulating factor, or rhu-interleukin 4, enhancement of erythropoietin-stimulated colony formation by erythroid and multipotential progenitor cells; (iii) modulatory actions of rhu-activin and rhu-inhibin are mediated through monocytes and T lymphocytes within the marrow; (iv) actions are apparent in the absence or presence of serum; and (v) rhu-activin and rhu-inhibin have no effect on colony formation by granulocyte-macrophage progenitor cells. This defines an indirect mode of action and a specificity for activin and inhibin on multipotential and erythroid progenitor cells.
authors.affiliation: Department of Medicine (Hematology/Oncology), Indiana University School of Medicine, Indianapolis 46223.
authors.forename: H E
authors.initials: HE
authors.lastname: Broxmeyer
authors.forename: L
authors.initials: L
authors.lastname: Lu
authors.forename: S
authors.initials: S
authors.lastname: Cooper
authors.forename: R H
authors.initials: RH
authors.lastname: Schwall
authors.forename: A J
authors.initials: AJ
authors.lastname: Mason
authors.forename: K
authors.initials: K
authors.lastname: Nikolics
mesh_terms: D028341:Activins; D001853:Bone Marrow / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003470:Culture Media; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D007265:Inhibins / Q000494:pharmacology / Q000502:physiology*; D011994:Recombinant Proteins / Q000494:pharmacology
chemical_list: D003470:Culture Media; D011994:Recombinant Proteins; D028341:Activins; D007265:Inhibins