title: A site-specific single strand endonuclease activity induced by NYs-1 virus infection of a Chlorella-like green alga.
abstract: A site-specific endonuclease was isolated from a eukaryotic Chlorella-like green alga infected with the dsDNA-containing virus NYs-1. The enzyme recognizes the sequence 5'-CC-3' and cleaves 5' to the first C. It cleaves 5'-CmC-3' sequences but not 5'-mCC-3' sequences. The enzyme creates breaks in dsDNA whenever two 5'-CC-3' sequences on opposite strands are close enough for the two strands to separate; when the 5'-CC-3' sequences on opposite strands are further apart only a portion of the strands separate. Consequently, NYs-1 endonuclease does not produce a completely stable DNA digestion pattern. The enzyme probably does not cleave ssDNA and definitely does not cleave ssRNA or dsRNA.
authors.affiliation: Department of Plant Pathology, University of Nebraska, Lincoln 68583-0722.
authors.forename: Y N
authors.initials: YN
authors.lastname: Xia
authors.forename: R
authors.initials: R
authors.lastname: Morgan
authors.forename: I
authors.initials: I
authors.lastname: Schildkraut
authors.forename: J L
authors.initials: JL
authors.lastname: Van Etten
mesh_terms: D002708:Chlorella / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D004277:DNA, Single-Stranded; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D015257:DNA-Cytosine Methylases / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000302:isolation & purification; D004790:Enzyme Induction; D008745:Methylation; D010942:Plant Viruses / Q000201:enzymology* / Q000235:genetics
chemical_list: D004277:DNA, Single-Stranded; D004279:DNA, Viral; D015257:DNA-Cytosine Methylases