title: Identification of two distinct protein carboxyl methyltransferases in eucaryotic cells.
abstract: Two distinct protein carboxyl methyltransferases (PCM) were identified in the electric organ of Torpedo ocellata. They were separated from each other in the active form by means of nondenaturing gel electrophoresis and by p-(chloromercuri)benzoate-agarose chromatography, and were individually identified by specific polyclonal antibodies. The existence of at least two distinct PCMs in eucaryotic cells raises the possibility that these enzymes are involved in distinct transmethylation reactions.
authors.affiliation: Department of Biochemistry, George S. Wise Faculty of Life Sciences, Tel Aviv University, Israel.
authors.forename: R
authors.initials: R
authors.lastname: Solomon
authors.forename: O
authors.initials: O
authors.lastname: Katzir
authors.forename: Y
authors.initials: Y
authors.lastname: Egozi
authors.forename: Y
authors.initials: Y
authors.lastname: Kloog
mesh_terms: D000818:Animals; D004557:Electric Organ / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel; D007106:Immune Sera; D015151:Immunoblotting; D007527:Isoenzymes / Q000276:immunology / Q000302:isolation & purification*; D008970:Molecular Weight; D011496:Protein Methyltransferases / Q000302:isolation & purification*; D011497:Protein O-Methyltransferase / Q000276:immunology / Q000302:isolation & purification*; D014101:Torpedo
chemical_list: D007106:Immune Sera; D007527:Isoenzymes; D011496:Protein Methyltransferases; D011497:Protein O-Methyltransferase