title: Microtubule-associated organelle and vesicle transport in fibroblasts.
abstract: Allen Video-enhanced contrast/differential interference contrast (AVEC-DIC) microscopy was used in conjunction with video intensification immunofluorescence microscopy to demonstrate that organelles and vesicle (particles) can move in either direction along microtubular linear elements in fibroblasts [Hayden et al., 1983]. Since it is not possible to determine the number of microtubules making up a linear element with light microscopy alone, AVEC-DIC microscopy was used in conjunction with whole-mount electron microscopy to show bidirectional transport along a single microtubule [Hayden and Allen, 1984]. These studies demonstrate that the structural polarity of the microtubule does not determine the direction of particle motion, and since dynein is an asymetric molecule, a simple microtubule-dynein-particle hypothesis cannot explain bidirectional transport along a single microtubule. Very little is known about regulation of particle transport in most cell types. Human embryonic lung fibroblasts grown on glass coverslips were serum-deprived for 24 hours and re-fed with serumless medium; the particle translocations/5 minutes were then determined. The cells were then re-fed with either serumless medium, serum-containing medium, or serumless medium containing some bioactive factor, and the particle translocations/5 minutes were again determined for the same cells. Medium containing 10% fetal bovine serum inhibited particle translocation by 51.8%. Of the bioactive factors tested, only vasopressin produced a significant reduction in particle translocations (38%). This suggests that protein kinase C or calcium/calmodulin kinase could be involved in regulating particle transport.
authors.affiliation: Siena College, Loudonville, NY 12211.
authors.forename: J H
authors.initials: JH
authors.lastname: Hayden
mesh_terms: D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology* / Q000648:ultrastructure; D006801:Humans; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D008857:Microscopy, Interference; D008870:Microtubules / Q000648:ultrastructure*; D015388:Organelles; D014741:Video Recording