title: Osteogenesis in in vivo diffusion chamber cultures of human marrow cells.
abstract: The osteogenic diffusion chamber culture of rodent marrow cells is a well established system. In the present study, marrow cells from children and adult human donors were incubated in diffusion chambers implanted intraperitoneally in athymic mice. After 4 or 8 weeks, the chamber content was examined by light and electron microscopy. Child-cell cultures showed osteogenic tissue consisting of a mineralizing fibrous component and cartilage. Ultrastructurally, the fibrous tissue was similar to osteoid and exhibited osteoblast-like cells and mineralizing nodules. Mineral aggregates were also found in the cartilage. These features in child-cell chambers were similar to those found in control chambers of rabbit marrow cells. Adult-cell chambers showed only unmineralized fibrous tissue. These results render previous findings in animal-cell diffusion chamber systems relevant to the understanding of bone formation in man. It is suggested that the difference between child- and adult-cell chambers reflects an age-related decline in the number of marrow osteoprogenitor cells or their potential to undergo terminal osteogenic differentiation.
authors.affiliation: Division of Oral Pathology, Hebrew University-Hadassah Faculty of Dental Medicine, Jerusalem, Israel.
authors.forename: I
authors.initials: I
authors.lastname: Bab
authors.forename: L
authors.initials: L
authors.lastname: Passi-Even
authors.forename: D
authors.initials: D
authors.lastname: Gazit
authors.forename: E
authors.initials: E
authors.lastname: Sekeles
authors.forename: B A
authors.initials: BA
authors.lastname: Ashton
authors.forename: N
authors.initials: N
authors.lastname: Peylan-Ramu
authors.forename: I
authors.initials: I
authors.lastname: Ziv
authors.forename: M
authors.initials: M
authors.lastname: Ulmansky
mesh_terms: D000328:Adult; D000818:Animals; D001854:Bone Marrow Cells; D002113:Calcification, Physiologic; D002356:Cartilage / Q000166:cytology / Q000502:physiology; D002478:Cells, Cultured; D002648:Child; D015194:Diffusion Chambers, Culture; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008854:Microscopy, Electron; D008903:Minerals / Q000378:metabolism; D010012:Osteogenesis; D010529:Peritoneal Cavity; D011817:Rabbits
chemical_list: D008903:Minerals