title: The use of amphotericin B in prevention of Trypanosoma theileri growth in bovine cell culture.
abstract: Trypanosoma theileri is an ubiquitous blood parasite of cattle. This parasite grows easily in RPMI medium and perturbs lymphocyte tests based on [3H]thymidine uptake. This paper reports that 0.5 microgram ml-1 amphotericin B in the medium is adequate to get Trypanosoma growth inhibition without alteration of either mitogenic or allogenic stimulation of bovine lymphoid cells in vitro.
authors.affiliation: Département de Microbiologie, Facultés Universitaires Notre-Dame de la Paix, Namur, Belgium.
authors.forename: N
authors.initials: N
authors.lastname: Lostrie-Trussart
authors.forename: J J
authors.initials: JJ
authors.lastname: Letesson
authors.forename: C
authors.initials: C
authors.lastname: Didembourg
authors.forename: A
authors.initials: A
authors.lastname: Depelchin
mesh_terms: D000666:Amphotericin B / Q000494:pharmacology*; D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000276:immunology / Q000469:parasitology*; D008934:Mitogens / Q000494:pharmacology; D013936:Thymidine / Q000378:metabolism; D014345:Trypanosoma / Q000187:drug effects / Q000254:growth & development*
chemical_list: D008934:Mitogens; D000666:Amphotericin B; D013936:Thymidine