title: Characterization of a pseudocholinesterase purified from surgeonfish tissues confirms the atypical nature of this enzyme.
abstract: The sialated, presumed-globular form of an atypical pseudocholinesterase (pseudo-ChE) previously described from surgeonfish tissues (Leibel: Comparative Biochemistry and Physiology 1988) has been purified to apparent homogeneity using a combination of salt fractionation along with ion-exchange and concanavalin A-Sepharose affinity chromatographic techniques. An overall 1,400-fold purification has been achieved with a 24% final yield of a cholinesterase (ChE) whose final specific activity is 50 mumol/min-mg. The purified enzyme was subjected to detailed biochemical and physical analysis. The purified pseudo-ChE is a sialated, globular, tetrameric enzyme with an apparent sedimentation coefficient of 11.5 S (+/- 0.5 S) and a molecular weight of 250 kilodaltons. The monomers are apparently not secured by disulfide bridges. The enzyme preferentially hydrolyzes acetyl(thio)choline but also hydrolyzes propionyl(thio)choline at reduced but comparable rates along with a wide variety of other noncholine esters. As such, it demonstrates the relative nonspecificity associated with classical pseudo-ChEs. However, the enzyme exhibits limited, but real, substrate inhibition with all choline esters as does true acetylcholinesterase (AChE). The enzyme is insensitive to the AChE inhibitor BW 284C51, sensitive to one (RO2-0683) of two (RO2-1250) pseudo-ChE inhibitors, and particularly sensitive to paraoxon inhibition (10(3)-10(4)-fold more so than AChE). It exhibits the short thermal half-life characteristic of pseudo-ChEs but not the expected ionic activation/inhibition profile. It is clear from this and other studies of atypical extrasynaptic cholinesterase activities occurring in other vertebrates that the orthodox categorization of cholinesterase as either "true" ("specific"; E.C. 3.1.1.7) or "pseudo" ("nonspecific"; E.C. 3.1.1.8) is inadequate to accommodate the increasing instances of ChE activities that exhibit atypical, intermediate properties.
authors.affiliation: Department of Biology, Lafayette College, Easton, Pennsylvania 18042.
authors.forename: W S
authors.initials: WS
authors.lastname: Leibel
mesh_terms: D000122:Acetylthiocholine / Q000378:metabolism; D000818:Animals; D002091:Butyrylcholinesterase / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism; D002499:Centrifugation, Density Gradient; D002800:Cholinesterase Inhibitors / Q000494:pharmacology; D002802:Cholinesterases / Q000032:analysis*; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D004592:Electrophoresis, Starch Gel; D005399:Fishes / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007700:Kinetics; D008970:Molecular Weight; D013860:Thiocholine / Q000031:analogs & derivatives / Q000378:metabolism
chemical_list: D002800:Cholinesterase Inhibitors; C009063:propionylthiocholine; D000122:Acetylthiocholine; D013860:Thiocholine; D002091:Butyrylcholinesterase; D002802:Cholinesterases