title: Changes in rapidly transported proteins in developing hamster retinofugal axons.
abstract: Proteins synthesized in retinal ganglion cells and conveyed to the terminals of optic tract axons in the rapid phase of axonal transport were analyzed at different developmental stages in the hamster. Animals between 2 d of age and adulthood were labeled intraocularly with 35S-methionine, and after a 4 hr survival time, the superior colliculus was dissected out, subjected to subcellular fractionation, and radiolabeled proteins in the particulate fraction analyzed by 2-dimensional gel electrophoresis and fluorography. The previously identified growth-associated phosphoprotein, GAP-43 (GAP-48, B-50, F1, pp46), was synthesized and transported at high levels in the neonate, but these levels declined precipitously after the second postnatal week. Immunohistochemical studies using a monospecific antibody showed that GAP-43 was localized along the entire length of retinal axons in the optic tract and target areas in P2 animals but was virtually absent in the adult visual pathway. By metabolic labeling, 2 proteins with molecular weights of about 230 kDa also showed a sharp decrease during development. In contrast, acidic proteins of 27 and 64 kDa, which were barely detectable in the neonate, increased steadily to become the most heavily labeled proteins of rapid axonal transport by the second postnatal week. Another group of proteins, of about 94-110 kDa, also rose to peak levels after birth but then declined. Temporal correlations between the molecular changes described here and the known anatomical events in optic tract development suggest that the synthesis and transport of particular membrane proteins may be directly related to the sequence of morphological changes.
authors.affiliation: Department of Brain and Cognitive Sciences, Whitaker College, Massachusetts Institute of Technology, Cambridge 02139.
authors.forename: K L
authors.initials: KL
authors.lastname: Moya
authors.forename: L I
authors.initials: LI
authors.lastname: Benowitz
authors.forename: S
authors.initials: S
authors.lastname: Jhaveri
authors.forename: G E
authors.initials: GE
authors.lastname: Schneider
mesh_terms: D000818:Animals; D001369:Axons / Q000378:metabolism*; D001692:Biological Transport; D006224:Cricetinae; D019922:GAP-43 Protein; D007150:Immunohistochemistry; D008565:Membrane Proteins / Q000378:metabolism; D008647:Mesocricetus; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D012160:Retina / Q000254:growth & development / Q000378:metabolism* / Q000648:ultrastructure; D013477:Superior Colliculi / Q000378:metabolism; D013997:Time Factors; D014795:Visual Pathways / Q000254:growth & development / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D019922:GAP-43 Protein; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins