title: Effect of 1,2-propanediol and dimethylsulphoxide on the meiotic spindle of the mouse oocyte.
abstract: At ovulation, the mouse oocyte is arrested at metaphase of the second meiotic division. Since microtubules are thermo-and chemosensitive structures, the effects of 1.5 M dimethylsulphoxide and 1.5 M 1,2-propanediol were studied at room temperature on the morphology of the meiotic spindle. Oocytes incubated at 37 degrees C or at room temperature served to estimate the effect of temperature in the experiment. The meiotic spindle was visualized by immunogold-silver staining of microtubules. In the control group at 37 degrees C, 88% of oocytes had normal spindles. After incubation at room temperature for the same time, 89% of oocytes showed abnormal spindles. In the oocytes exposed to dimethyl-sulphoxide or 1,2-propanediol at room temperature a protective effect on spindle morphology could be recognized. Subsequent incubation at 37 degrees C resulted in partial restoration of the observed abnormalities after cooling to room temperature and after exposure to dimethylsulphoxide. Incubation at 37 degrees C after exposure to 1,2-propanediol at room temperature induced spindle absence in the majority of oocytes. Although this latter condition allowed fertilization without increased incidence of ploidy abnormalities, a role for 1,2-propanediol as an activating agent is hypothesized.
authors.affiliation: Centre for Reproductive Medicine, Vrije Universiteit Brussel, Belgium.
authors.forename: J
authors.initials: J
authors.lastname: Van der Elst
authors.forename: E
authors.initials: E
authors.lastname: Van den Abbeel
authors.forename: R
authors.initials: R
authors.lastname: Jacobs
authors.forename: E
authors.initials: E
authors.lastname: Wisse
authors.forename: A
authors.initials: A
authors.lastname: Van Steirteghem
mesh_terms: D000818:Animals; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D005260:Female; D066298:In Vitro Techniques; D008540:Meiosis / Q000187:drug effects; D051379:Mice; D009865:Oocytes / Q000187:drug effects*; D019946:Propylene Glycol; D011409:Propylene Glycols / Q000494:pharmacology*
chemical_list: D011409:Propylene Glycols; D019946:Propylene Glycol; D004121:Dimethyl Sulfoxide