Histological analysis of myleran induced oligodactyly of longitudinal deficiency in rats.
In this study, tibial ray deficiency was used as a model of longitudinal ray deficiency and the pathogenesis of the oligodactyly of longitudinal ray deficiency was investigated. Tibial ray deficiency was induced in rat fetuses by a single oral administration of myleran to pregnant rats on day 10 of pregnancy. The dose of myleran administered was 40 mg/kg of body weight. In order to observe the process of formation of the foot plates and digital rays of tibial ray deficiency, histological examination was done. The period of development studied was from day 12 to 16 of pregnancy. A series of fetuses was surgically removed from their mothers at 12 hours' interval. Histological examinations of the hind limbbuds and foot plates were performed quantitatively. The formation of hind limbbuds was detected on day 12 in myleran treated group as in control group. The formation of digital rays in the myleran treated group was initially observed on day 14.5, 12 hours later than control. The size of hind limbbuds and the density of mesenchymal cells in hind limbbuds treated by myleran was smaller than control and the hypoplasia of the limbbuds was maximum on day 14. The shape of foot plates treated by myleran was asymmetrical compared with the symmetrical limbbuds in controls. Concerning tissue damage in limbbuds, dead cells of ectoderm were distributed on the top of the limbbuds on day 12 and they disappeared 24 hours later. On the other hand, dead cells of mesenchyme were scattered in hind limbbuds on day 12.(ABSTRACT TRUNCATED AT 250 WORDS)
Ogino
T
T
Department of Orthopedic Surgery, Hokkaido University, School of Medicine, Sapporo/Japan.
Kato
H
H
D000014:Abnormalities, Drug-Induced / Q000196:embryology / Q000209:etiology* / Q000473:pathology; D000818:Animals; D002066:Busulfan / Q000009:adverse effects*; D005260:Female; D005385:Fingers / Q000002:abnormalities*; D011247:Pregnancy; D051381:Rats; D011919:Rats, Inbred Strains
D002066:Busulfan