Phospholipid analysis of human eosinophils: high levels of alkylacylglycerophosphocholine (PAF precursor).
The phospholipid composition and fatty acid profiles of human eosinophils were studied. Extremely high levels of ether phospholipids were found in this type of cell, such as alkylacylglycerophosphocholine (GPC) and alkenylacylglycerophosphoethanolamine (GPE); these two ether phospholipids accounted for about three-fourths of the choline and ethanolamine glycerophospholipids (CGP and EGP), respectively. Fatty acid analyses revealed that very large portions of arachidonic acid (20:4) were esterified to alkylacyl-GPC (92.0% in CGP) and alkenylacyl-GPE (86.6% in EGP), respectively. While high amounts of alkylacyl-GPC and the abundance of 20:4 in this ether phospholipid have been observed in other types of blood cells of various animals, these percentages for human eosinophils are the highest among such cells. These results suggest that alkyl and alkenyl ether phospholipids play essential roles in human eosinophils in various physiological and pathological conditions.
Ojima-Uchiyama
A
A
Faculty of Pharmaceutical Sciences, Teikyo University, Kanagawa, Japan.
Masuzawa
Y
Y
Sugiura
T
T
Waku
K
K
Saito
H
H
Yui
Y
Y
Tomioka
H
H
D004804:Eosinophils / Q000032:analysis*; D005227:Fatty Acids / Q000032:analysis; D006801:Humans; D009504:Neutrophils / Q000032:analysis; D010713:Phosphatidylcholines / Q000032:analysis; D010714:Phosphatidylethanolamines / Q000032:analysis; D010743:Phospholipids / Q000032:analysis*; D010972:Platelet Activating Factor / Q000031:analogs & derivatives* / Q000032:analysis
C036761:1-alkyl-2-acyl-sn-glycero-3-phosphocholine; D005227:Fatty Acids; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010743:Phospholipids; D010972:Platelet Activating Factor