The clastogenic activity of 1,6-dinitropyrene in peripheral human lymphocytes.
The ability of 1,6-dinitropyrene to induce chromosome damage in peripheral human lymphocyte cultures has been demonstrated. Low levels of clastogenic activity were detected following 3-h treatments with 1,6-dinitropyrene in the presence of a rat-liver cytosol fraction. The clastogenic activity reached a peak at a concentration of 1.25 micrograms/ml of 1,6-dinitropyrene after which the frequency of aberrations decreased. This unusual genotoxic dose response is similar to that found previously in yeast and rat-liver cells. The fact that a positive result was obtained using human lymphocytes shows that, in the presence of the appropriate activation system, dinitropyrene is genotoxic in human cells.
Adams
K
K
Biomedical and Physiological Research Group, School of Biological Sciences, University College of Swansea, Great Britain.
Lafi
A
A
Parry
J M
JM
D002875:Chromosomes / Q000187:drug effects*; D003600:Cytosol / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008214:Lymphocytes / Q000648:ultrastructure; D009153:Mutagens; D011721:Pyrenes / Q000494:pharmacology*
D009153:Mutagens; D011721:Pyrenes; C033309:1,6-dinitropyrene