title: Phenotypic instability of tumor cell clones: clonal expression of Fc receptors in the human leukemic K562 cell line and its relationship with hemoglobin expression.
abstract: Membrane expression of Fc receptors (FcR) was studied in clones of human K562 cells during short- and long-term culture. Using a manual cloning method, well-defined clones were generated either from FcR-positive or FcR-negative cells. The fourth day after cloning, the majority of cloned cells manifested shifts in FcR expression without evidence of an orderly pattern. After long-term culture (about 34 passages), most clones expressed FcR values close to those found in the cell line. In addition, the selection of six clones expressing a stable FcR phenotype suggested that the presence of FcR is correlated to a low hemoglobinization of the multipotential K562 cells.
authors.affiliation: Faculté de Pharmacie, Laboratoire de Biochimie, Reims, France.
authors.forename: P
authors.initials: P
authors.lastname: Jeannesson
authors.forename: B
authors.initials: B
authors.lastname: Gérard
authors.forename: J C
authors.initials: JC
authors.lastname: Jardillier
authors.forename: J
authors.initials: J
authors.lastname: Bernard
mesh_terms: D002454:Cell Differentiation; D002999:Clone Cells; D004912:Erythrocytes / Q000276:immunology; D006454:Hemoglobins / Q000235:genetics*; D006801:Humans; D007700:Kinetics; D007938:Leukemia / Q000235:genetics*; D010641:Phenotype; D011961:Receptors, Fc / Q000235:genetics*; D012397:Rosette Formation; D014407:Tumor Cells, Cultured
chemical_list: D006454:Hemoglobins; D011961:Receptors, Fc