Rapid extraction of oxazepam from greyhound urine for high performance liquid chromatography analysis.
A rapid and efficient procedure is described for the extraction and analysis of oxazepam, the major urinary metabolite of diazepam in greyhounds. Urine was extracted by passing through a bonded silica column (Bond Elut) following enzyme hydrolysis. The adsorbed drug was eluted and then detected and measured by high performance liquid chromatography (HPLC). Recoveries were in excess of 85% at 50 ng/ml concentrations. Detection was possible up to 30 h after a single oral dose of diazepam (5 mg).
Moore
C M
CM
Department of Forensic Medicine and Science, University of Glasgow, U.K.
Oliver
J S
JS
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004285:Dogs / Q000652:urine*; D004300:Doping in Sports; D010076:Oxazepam / Q000302:isolation & purification / Q000652:urine*
D010076:Oxazepam