title: Human organ culture techniques for the detection and evaluation of genotoxic agents.
abstract: In order to obtain information on the genotoxic metabolism of carcinogens in human skin in vivo, model in-vitro systems have been developed to mimic in-vivo metabolism qualitatively. Direct labelling (3H and 14C) and 32P-postlabelling analyses of benzo[alpha]pyrene (BP)-DNA adducts in human skin explants, CD1 mouse explants and CD1 mouse skin in vivo have thus allowed comparisons of the genotoxic metabolism of BP in mouse and human skin.
authors.affiliation: Shell Research Ltd, Sittingbourne Research Centre, Kent, UK.
authors.forename: W P
authors.initials: WP
authors.lastname: Watson
authors.forename: R J
authors.initials: RJ
authors.lastname: Smith
authors.forename: K R
authors.initials: KR
authors.lastname: Huckle
authors.forename: A S
authors.initials: AS
authors.lastname: Wright
mesh_terms: D000818:Animals; D001564:Benzo(a)pyrene / Q000378:metabolism*; D004247:DNA / Q000378:metabolism*; D005260:Female; D006801:Humans; D051379:Mice; D009924:Organ Culture Techniques; D010761:Phosphorus Radioisotopes; D012867:Skin / Q000378:metabolism*
chemical_list: D010761:Phosphorus Radioisotopes; D001564:Benzo(a)pyrene; D004247:DNA