Selective quantitative determination of tobramycin from fermentation broth.
A derivative of Rhizobium meliloti 41 was constructed (strain GY654) which was resistant to apramycin and kanamycin but remained sensitive to tobramycin. Strain GY654 selectively measures tobramycin and 6"-O-carbamoyltobramycin in the presence of apramycin, kanamycin and 6"-O-carbamoylkanamycin B, therefore it is suitable for the rapid quantitation of 6"-O-carbamoyltobramycin and tobramycin in fermentation broths of Streptomyces tenebrarius and solvents containing antibiotic mixtures.
Kiss
G B
GB
Institute of Genetics, Hungarian Academy of Sciences, Szeged.
Széll
V
V
Ambrus
G
G
Ott
I
I
D001681:Biological Assay / Q000379:methods*; D002478:Cells, Cultured; D003470:Culture Media / Q000032:analysis*; D004352:Drug Resistance, Microbial; D012231:Rhizobium / Q000187:drug effects*; D014031:Tobramycin / Q000031:analogs & derivatives* / Q000032:analysis
D003470:Culture Media; C057745:6''-O-carbamoyltobramycin; D014031:Tobramycin