Detection of IgA antibodies and quantification of IgA antibody-producing cells specific for ovalbumin or Trichinella spiralis in the rat.
This report describes procedures to quantify IgA responses in the rat sensitized to ovalbumin or infected with the parasite Trichinella spiralis: an ELISPOT detecting specific IgA antibody-producing cells in lymph nodes, and an ELISA demonstrating IgA antibody in serum and gut mucosal scrapings. For this purpose a mouse monoclonal anti-rat IgA antibody was produced. This IgG1-kappa 1 antibody recognized rat IgA but not rat IgM, IgG, or IgE. It proved very suitable in both assays. Using this reagent we could demonstrate large numbers of IgA anti-ovalbumin-producing cells in the mesenteric lymph nodes 15 days after sensitization to ovalbumin via the Peyer's patches. At 28 days after sensitization the numbers were much lower. IgA antibody titres to ovalbumin in serum were maximal between days 14 and 21 after immunization. Maximal numbers of IgA anti-T. spiralis-producing cells were found in the mesenteric lymph nodes 12 days after infection with muscle larvae, followed by a sharp decrease at 15 days. Maximal IgA anti-T. spiralis antibody titres in serum and mucus scrapings of small intestines were found on days 10 and 12 after oral infection with the parasite.
Van Loveren
H
H
National Institute of Public Health and Environmental Protection, Bilthoven, The Netherlands.
Osterhaus
A D
AD
Nagel
J
J
Schuurman
H J
HJ
Vos
J G
JG
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis; D000918:Antibody Specificity; D000921:Antibody-Producing Cells / Q000032:analysis*; D000947:Antigens, Helminth / Q000276:immunology*; D002459:Cell Fusion; D007070:Immunoglobulin A / Q000032:analysis* / Q000096:biosynthesis; D007132:Immunoglobulin Isotypes / Q000032:analysis; D007413:Intestinal Mucosa / Q000032:analysis; D007958:Leukocyte Count; D008198:Lymph Nodes / Q000032:analysis; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000276:immunology*; D051381:Rats; D011919:Rats, Inbred Strains; D014234:Trichinella / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth; D007070:Immunoglobulin A; D007132:Immunoglobulin Isotypes; D010047:Ovalbumin