title: Structure elucidation of blood group B-like and I-active ceramide eicosa- and pentacosasaccharides from rabbit erythrocyte membranes by combined gas chromatography-mass spectrometry; electron-impact and fast-atom-bombardment mass spectrometry; and two-dimensional correlated, relayed-coherence transfer, and nuclear Overhauser effect 500-MHz 1H-n.m.r. spectroscopy.
abstract: The structures of two glycosphingolipids, a ceramide eicosasaccharide BIrab-3 and a ceramide pentacosasaccharide BIrab-4 with "B-like" and distinct I blood-group activity, isolated in high yield from rabbit erythrocyte membranes, were investigated. The determination of their general structure, alpha-D-Galp-(1----3)-beta-D-Galp-(1----4)-beta-D-GlcpNAc-(1----3)- [alpha-D-Galp-(1----3)-beta-D-Galp-(1----4)-beta-D-Glcp-NAc-(1----6)]-be ta- D-Galp-(1----n4)-beta-D-GlcpNAc-(1----3)-beta-D-Galp-(1----4)-beta-D-Gl cp- (1----1)-ceramide, with n = 3 for BIrab-3 and n = 4 for BIrab-4, was based on the results of methylation analysis, fast-atom-bombardment and electron-impact mass spectrometry, 1D and 2D COSY, RCT, and n.O.e. 1H-N.m.r. spectra, and specific enzymic and chemical degradation.
authors.affiliation: Institut für Experimentelle Hämatologie und Bluttransfusionswesen, Universität Bonn, Federal Republic of Germany.
authors.forename: P
authors.initials: P
authors.lastname: Hanfland
authors.forename: M
authors.initials: M
authors.lastname: Kordowicz
authors.forename: J
authors.initials: J
authors.lastname: Peter-Katalinić
authors.forename: H
authors.initials: H
authors.lastname: Egge
authors.forename: J
authors.initials: J
authors.lastname: Dabrowski
authors.forename: U
authors.initials: U
authors.lastname: Dabrowski
mesh_terms: D000017:ABO Blood-Group System / Q000032:analysis*; D000818:Animals; D001789:Blood Group Antigens / Q000032:analysis*; D002240:Carbohydrate Sequence; D002518:Ceramides / Q000097:blood*; D004910:Erythrocyte Membrane / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry; D006028:Glycosphingolipids / Q000032:analysis; D007048:I Blood-Group System / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D008969:Molecular Sequence Data; D011817:Rabbits
chemical_list: D000017:ABO Blood-Group System; D001789:Blood Group Antigens; D002518:Ceramides; D006028:Glycosphingolipids; D007048:I Blood-Group System