Astrocytes synthesize angiotensinogen in brain.
Cell types associated with angiotensinogen mRNA in rat brain were identified in individual brain sections by in situ hybridization with tritiated RNA probes or with a sulfur-35--labeled oligonucleotide combined with immunocytochemical detection of either glial fibrillary acidic protein (GFAP) for astrocytes or microtubule-associated protein (MAP-2) for neurons. Autoradiography revealed silver grains clustered primarily over GFAP-reactive soma and processes; most grain clusters were not associated with MAP-2--reactive cells. These results demonstrate that, in contrast to other known neuropeptide precursors, angiotensinogen is synthesized by glia.
Stornetta
R L
RL
Department of Pharmacology, University of Virginia School of Medicine, Charlottesville 22908.
Hawelu-Johnson
C L
CL
Guyenet
P G
PG
Lynch
K R
KR
D000808:Angiotensinogen / Q000096:biosynthesis* / Q000235:genetics; D000818:Animals; D001253:Astrocytes / Q000378:metabolism*; D001921:Brain / Q000378:metabolism*; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D006651:Histocytochemistry; D008869:Microtubule-Associated Proteins / Q000032:analysis; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D051381:Rats
D005904:Glial Fibrillary Acidic Protein; D008869:Microtubule-Associated Proteins; D012333:RNA, Messenger; D000808:Angiotensinogen