Influence of VIP on D-galactose transport across rabbit jejunum in vivo.
D-galactose absorption during 1 min perfusion periods was not affected by the presence of 10(-7)-10(-8) M VIP in the sugar solution, but exposure of mucosa to VIP for 5 min inhibited sugar absorption in the subsequent periods of perfusion. This inhibition is reversed after washing with saline solution. The effect of VIP disappeared when 10(-6) M RMI 12330A was added to the incubation solution together with 10(-7) M VIP and 1 mM D-galactose solution. These results suggest the existence of VIP receptors on the brush border membrane. The action of VIP could be mediated through the cAMP system.
Rodríguez-Yoldi
M J
MJ
Departamento de Biomedicina (Fisiología), Facultad de Veterinaria, Zaragoza, Spain.
Arruebo
M P
MP
Alcalde
A I
AI
Murillo
M D
MD
D000818:Animals; D005690:Galactose / Q000378:metabolism*; D007097:Imines / Q000494:pharmacology; D007408:Intestinal Absorption / Q000187:drug effects*; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism; D007583:Jejunum / Q000378:metabolism*; D008297:Male; D011817:Rabbits; D013997:Time Factors; D014660:Vasoactive Intestinal Peptide / Q000037:antagonists & inhibitors / Q000502:physiology*
D007097:Imines; D014660:Vasoactive Intestinal Peptide; C014925:RMI 12330A; D005690:Galactose