Skeletal muscle mitochondrial beta-oxidation. A study of the products of oxidation of [U-14C]hexadecanoate by h.p.l.c. using continuous on-line radiochemical detection.
Well-coupled mitochondrial fractions were prepared from rat skeletal muscle without the use of proteolytic enzymes. The products of [U-14C]hexadecanoate oxidation by rat skeletal muscle mitochondrial fractions were analysed by h.p.l.c. with on-line radiochemical detection. In the presence of 1 mM-carnitine, 70% of the products is acetylcarnitine. In agreement with Veerkamp et al. [Veerkamp, van Moerkerk, Glatz, Zuurveld, Jacobs & Wagenmakers (1986) Biochem. Med. Metab. Biol. 35, 248-259] 14CO2 release is shown to be an unreliable estimate of flux through beta-oxidation in skeletal muscle mitochondrial fractions. The flux through beta-oxidation is recorded unambiguously polarographically in the presence of 1 mM-carnitine and the absence of citrate cycle intermediates.
Watmough
N J
NJ
Department of Pharmacological Sciences, Medical School, University of Newcastle upon Tyne, U.K.
Bhuiyan
A K
AK
Bartlett
K
K
Sherratt
H S
HS
Turnbull
D M
DM
D000108:Acetylcarnitine / Q000378:metabolism; D000818:Animals; D002250:Carbon Radioisotopes; D002331:Carnitine / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D008293:Malates / Q000494:pharmacology; D008297:Male; D008931:Mitochondria, Muscle / Q000187:drug effects / Q000378:metabolism*; D010084:Oxidation-Reduction; D010169:Palmitic Acids / Q000378:metabolism*; D010172:Palmitoylcarnitine / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D014018:Tissue Distribution
D002250:Carbon Radioisotopes; D008293:Malates; D010169:Palmitic Acids; D010172:Palmitoylcarnitine; D000108:Acetylcarnitine; C030298:malic acid; D002331:Carnitine