title: Phenytoin-induced stress protein synthesis in mouse embryonic tissue.
abstract: Several proteins have been shown to be synthesized in response to various environmental stimuli, including treatment with teratogens. The role of these proteins in the teratogenic process is unknown. Pregnant A/J mice were treated with either a teratogenic or a non-teratogenic dose of the anticonvulsant drug, phenytoin (PHT). Protein synthesis in embryonic craniofacial (target) tissue or forelimb buds (non-target) was determined by incorporation of radiolabeled leucine and analysis by two-dimensional polyacrylamide gel electrophoresis. Synthesis of three proteins in target tissue and one protein in non-target tissue was stimulated by drug treatment. These results suggest that synthesis of specific stress proteins may serve as biomarkers of drug-target tissue interaction.
authors.affiliation: Division of Reproductive and Developmental Toxicology, National Center for Toxicological Research, Jefferson, Arkansas 72079.
authors.forename: D K
authors.initials: DK
authors.lastname: Hansen
authors.forename: J F
authors.initials: JF
authors.lastname: Anson
authors.forename: W G
authors.initials: WG
authors.lastname: Hinson
authors.forename: J L
authors.initials: JL
authors.lastname: Pipkin
mesh_terms: D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D004622:Embryo, Mammalian / Q000187:drug effects / Q000378:metabolism*; D005147:Facial Bones / Q000196:embryology; D005260:Female; D005552:Forelimb / Q000196:embryology; D006360:Heat-Shock Proteins / Q000096:biosynthesis* / Q000302:isolation & purification; D051379:Mice; D008805:Mice, Inbred A; D009687:Nuclear Proteins / Q000096:biosynthesis / Q000302:isolation & purification; D010672:Phenytoin / Q000633:toxicity; D011247:Pregnancy; D012886:Skull / Q000196:embryology; D013723:Teratogens / Q000633:toxicity
chemical_list: D006360:Heat-Shock Proteins; D009687:Nuclear Proteins; D013723:Teratogens; D010672:Phenytoin