title: A gene-cloning system for Kluyveromyces lactis and isolation of a chromosomal gene required for killer toxin production.
abstract: A transformation system derived from the circular plasmid pKD1 has been developed for Kluyveromyces lactis. The principle is essentially equivalent to that of the 2 microns/Saccharomyces cerevisiae transformation system. The main features of the system are presented. Using a pKD1-based DNA bank of K. lactis, the KEX1 gene involved in the killer system was isolated by complementation.
authors.affiliation: Institut Curie, Centre Universitaire Paris-Sud, Orsay, France.
authors.forename: X J
authors.initials: XJ
authors.lastname: Chen
authors.forename: M
authors.initials: M
authors.lastname: Wésolowski-Louvel
authors.forename: C
authors.initials: C
authors.lastname: Tanguy-Rougeau
authors.forename: M M
authors.initials: MM
authors.lastname: Bianchi
authors.forename: L
authors.initials: L
authors.lastname: Fabiani
authors.forename: M
authors.initials: M
authors.lastname: Saliola
authors.forename: C
authors.initials: C
authors.lastname: Falcone
authors.forename: L
authors.initials: L
authors.lastname: Frontali
authors.forename: H
authors.initials: H
authors.lastname: Fukuhara
mesh_terms: D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000235:genetics; D005656:Fungal Proteins / Q000096:biosynthesis / Q000235:genetics*; D005800:Genes, Fungal; D005822:Genetic Vectors; D055646:Killer Factors, Yeast; D007716:Kluyveromyces / Q000235:genetics* / Q000378:metabolism; D009183:Mycotoxins / Q000096:biosynthesis / Q000235:genetics*; D010957:Plasmids; D011401:Promoter Regions, Genetic; D029701:Saccharomyces cerevisiae Proteins; D004718:Saccharomycetales / Q000235:genetics*; D014170:Transformation, Genetic
chemical_list: D004271:DNA, Fungal; D005656:Fungal Proteins; C527157:KHR1 protein, S cerevisiae; D055646:Killer Factors, Yeast; D009183:Mycotoxins; D029701:Saccharomyces cerevisiae Proteins