Design and development of a single-reagent polarization fluoroimmunoassay for methamphetamine.
The design and development of reagents suitable for use in single-reagent polarization fluoroimmunoassay of methamphetamine is described. The single-reagent method requires that a substantial fraction of antibodies exhibit fast dissociation of bound tracer. Antiserum obtained early in the course of immunization of a sheep with a methamphetamine immunogen had suitable dissociation characteristics. Tracers with bridges of different length between methamphetamine and the fluorescein label group were prepared and evaluated. A single-reagent assay with sensitivity and specificity suitable for urine screening application was demonstrated.
Eremin
S A
SA
Department of Chemistry, Moscow State University, U.S.S.R.
Schiavetta
D E
DE
Lotey
H
H
Smith
D S
DS
Landon
J
J
D000818:Animals; D005260:Female; D015200:Fluoroimmunoassay / Q000379:methods*; D006801:Humans; D007202:Indicators and Reagents; D008694:Methamphetamine / Q000032:analysis* / Q000276:immunology; D012756:Sheep
D007202:Indicators and Reagents; D008694:Methamphetamine