Isolation and characterisation of GLP-1 7-36 amide from rat intestine. Elevated levels in diabetic rats.
Glucagon-like peptide-1 (GLP-1) was purified to homogeneity by HPLC and anion-exchange chromatography. A molecular mass of 3297.4 Da was obtained by FAB mass spectrometry which corresponded exactly to GLP-1 7-36 NH2, providing evidence that amidation occurs at an arginine residue during the post-translational processing of GLP-1. The distribution of GLP-1 7-36 NH2-like immunoreactivity (GLP-1 7-36 NH2 IR) was determined in the rat gastrointestinal tract. Highest concentrations were found in terminal ileum and colon. Streptozocin-induced diabetic rats, who showed a significant increase in food intake, had a significant increase of GLP-1 7-36 NH2 IR in the colon.
Kreymann
B
B
Department of Medicine, Royal Postgraduate Medical School, Hammersmith Hospital, London, England.
Yiangou
Y
Y
Kanse
S
S
Williams
G
G
Ghatei
M A
MA
Bloom
S R
SR
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D003106:Colon / Q000032:analysis / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D004435:Eating; D005934:Glucagon; D052216:Glucagon-Like Peptide 1; D004763:Glucagon-Like Peptides; D007082:Ileum / Q000032:analysis / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000032:analysis; D013058:Mass Spectrometry; D008970:Molecular Weight; D010446:Peptide Fragments; D010455:Peptides / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D014018:Tissue Distribution
D010446:Peptide Fragments; D010455:Peptides; C055453:glucagon-like peptide 1 (7-36)amide; D004763:Glucagon-Like Peptides; D052216:Glucagon-Like Peptide 1; D005934:Glucagon