cDNA cloning, nucleotide sequence and expression of the gene for arylsulfatase in the sea urchin (Hemicentrotus pulcherrimus) embryo.
Arylsulfatase is known to be synthesized in large amounts at the early gastrula stage of sea urchin development. We determined the amino acid sequence of a portion of the purified sea urchin embryonic arylsulfatase, and then isolated a cDNA clone for arylsulfatase by screening a sea urchin plutei lambda gt10 cDNA library with an oligodeoxynucleotide probe synthesized according to the determined amino acid sequence. The longest cDNA clones were selected and the nucleotide sequence determined. The cDNA is 2422 nucleotides long and encodes 551 amino acids. The deduced amino acid sequence has not sequence similarity with any of the peptides registered in NBRF peptide databank. Northern blot analysis revealed that the arylsulfatase cDNA hybridizes to a 2.9-kb mRNA. This mRNA exists in the unfertilized egg in small amounts, but markedly increases after the blastula stage preceding the increase of the arylsulfatase activity.
Sasaki
H
H
Zoological Institute, Faculty of Science, University of Tokyo, Japan.
Yamada
K
K
Akasaka
K
K
Kawasaki
H
H
Suzuki
K
K
Saito
A
A
Sato
M
M
Shimada
H
H
D000595:Amino Acid Sequence; D000818:Animals; D001192:Arylsulfatases / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification*; D005786:Gene Expression Regulation; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D010063:Ovum / Q000201:enzymology*; D012333:RNA, Messenger / Q000302:isolation & purification; D012617:Sea Urchins / Q000254:growth & development; D013429:Sulfatases / Q000235:genetics*
D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D004247:DNA; D013429:Sulfatases; D001192:Arylsulfatases