The influence of protein deficiency on immunity to Heligmosomoides polygyrus (Nematoda) in mice.
The influence of dietary protein on the efficiency with which mice could be immunized against infection with the nematode Heligomosomoides polygyrus was investigated. Immunization with irradiated larvae did not protect outbred mice fed synthetic diets containing 2% or 4% protein against a challenge infection, while animals fed a diet containing 8% protein were significantly resistant. In further experiments with high-responder NIH mice, protein malnutrition was again found to cause a significant depression in immunity. Immunization primed all mice for an intense production of antibody against larval worms in a challenge infection, and although a slightly higher titre of antibody was detected in the plasma of mice fed a 16% compared with a 2% protein diet it seemed unlikely that this was sufficient to account for the reduced resistance of the malnourished mice. The development of eosinophilia in the blood of immunized mice was significantly delayed in malnourished animals following challenge, and it is suggested that a reduction in the number of granulocytes attacking larval worms contributed to the low level of resistance observed in these animals. Protein malnutrition thus markedly suppresses the effectiveness of immunization of mice against an intestinal nematode, and it is suggested that this result may be of general significance with regard to the potential for widespread immunization of people against infections of this type.
Slater
A F
AF
Department of Zoology, University of Oxford.
Keymer
A E
AE
D000704:Analysis of Variance; D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis*; D004044:Dietary Proteins / Q000008:administration & dosage; D004797:Enzyme-Linked Immunosorbent Assay; D004802:Eosinophilia; D005260:Female; D006369:Heligmosomatoidea / Q000276:immunology*; D007114:Immunization; D008297:Male; D051379:Mice; D009349:Nematode Infections / Q000276:immunology* / Q000517:prevention & control; D009351:Nematospiroides dubius / Q000276:immunology*; D009752:Nutritional Status; D011488:Protein Deficiency / Q000276:immunology*; D015430:Weight Gain; D015431:Weight Loss
D000909:Antibodies, Helminth; D004044:Dietary Proteins