title: Metal-induced alteration of the cell membrane/cytoplasm complex studied by flow cytometry and detergent lysis.
abstract: Flow cytometric analysis of the cell cycle is most effectively accomplished with membrane-/cytoplasm-free ("clean") nuclei. Non-ionic detergents (e.g. NP40 or Triton X-100) commonly are employed to solubilize cell membranes/cytoplasm to produce "clean" nuclei. Treatment of murine erythroleukemic cells (MELC) with tri-n-butyltin methoxide, cadmium acetate, zinc sulfate, or lead acetate alters the properties of the cell membrane/cytoplasm complex making it resistant to NP40 dissolution. On a molar basis, the organotin compound was more effective in inducing resistance to detergent-mediated dissolution than the inorganic metal compounds. Resistance to NP40-mediated dissolution was manifested as an increase in the flow cytometric parameters 90 degrees scatter and fluorescein isothiocyanate (FITC) fluorescence and was confirmed by light microscopy.
authors.affiliation: NSI, Environmental Sciences, Research Triangle Park, NC 27709.
authors.forename: R M
authors.initials: RM
authors.lastname: Zucker
authors.forename: K H
authors.initials: KH
authors.lastname: Elstein
authors.forename: R E
authors.initials: RE
authors.lastname: Easterling
authors.forename: E J
authors.initials: EJ
authors.lastname: Massaro
mesh_terms: D000085:Acetates; D000818:Animals; D002104:Cadmium / Q000633:toxicity; D002453:Cell Cycle / Q000187:drug effects; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects*; D002467:Cell Nucleus / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003593:Cytoplasm / Q000187:drug effects*; D003902:Detergents / Q000494:pharmacology; D000431:Ethanol / Q000494:pharmacology; D005434:Flow Cytometry; D008670:Metals / Q000633:toxicity*; D051379:Mice; D009942:Organometallic Compounds / Q000633:toxicity; D013431:Sulfates / Q000633:toxicity; D014220:Trialkyltin Compounds / Q000633:toxicity; D015032:Zinc / Q000633:toxicity; D019287:Zinc Sulfate
chemical_list: D000085:Acetates; D003902:Detergents; D008670:Metals; D009942:Organometallic Compounds; D013431:Sulfates; D014220:Trialkyltin Compounds; D002104:Cadmium; D000431:Ethanol; C011559:tributyltin; D019287:Zinc Sulfate; C028031:cadmium acetate; D015032:Zinc; C008261:lead acetate