(Pyridoxylated hemoglobin)-(polyoxyethylene) conjugate solution as blood substitute for normothermic whole body rinse-out.
In order to investigate a new possibility for artificial blood with oxygen-carrying capability to be applied to other than mere supplementation, normothermic whole body rinse-out in which artificial blood deriving from perfluorochemical emulsion, Fluosol-DA 20% (Green Cross Co., Ltd., Osaka, Japan) or stabilized hemoglobin solution, (pyridoxylated hemoglobin)-(polyoxyethylene) conjugate solution (Ajinomoto Co., Ltd., Tokyo, Japan) were used as rinsing fluid for a blood purification experiment. Replacement either with approximately 150 ml/kg of Fluosol-DA or stabilized hemoglobin solution showed effective removal of digoxin at a reduction rate of 96.3% or 92.2%, respectively. However, when Fluosol-DA was used, a certain amount of perfluorochemical should be retrieved by centrifugation to avoid a possible toxic effect on the reticulo-endothelial system. Even though 3 out of 6, and 3 out of 8 dogs, respectively, survived for a long period after the procedure, the experimental dogs were very susceptible to infection.
Agishi
T
T
Department of Surgery, Kidney Center, Tokyo Women's Medical College, Japan.
Funakoshi
Y
Y
Honda
H
H
Yamagata
K
K
Kobayashi
M
M
Takahashi
M
M
D000818:Animals; D001778:Blood Coagulation Disorders / Q000139:chemically induced; D001802:Blood Substitutes / Q000627:therapeutic use* / Q000633:toxicity; D056486:Chemical and Drug Induced Liver Injury; D004077:Digoxin / Q000097:blood / Q000633:toxicity; D004285:Dogs; D004338:Drug Combinations / Q000627:therapeutic use / Q000633:toxicity; D005078:Exchange Transfusion, Whole Blood; D005466:Fluorocarbons / Q000627:therapeutic use / Q000633:toxicity; D006400:Hematocrit; D006454:Hemoglobins / Q000378:metabolism; D006895:Hydroxyethyl Starch Derivatives; D007674:Kidney Diseases / Q000139:chemically induced; D011092:Polyethylene Glycols / Q000378:metabolism; D012996:Solutions
D001802:Blood Substitutes; D004338:Drug Combinations; D005466:Fluorocarbons; D006454:Hemoglobins; D006895:Hydroxyethyl Starch Derivatives; D012996:Solutions; C050907:pyridoxalated-hemoglobin-polyethylene glycol conjugate; D011092:Polyethylene Glycols; D004077:Digoxin; C017323:glucose, glycerol, hydroxyethyl starch, perfluorodecalin, perfluorotripropylamine, pluronic F-68, salts, yolk phospholipids drug combination