Isoenzyme patterns of pyruvate kinase, lactate dehydrogenase, and alkaline phosphatase in isolated fat-storing cells of rat liver.
Fat-storing cells and other non-parenchymal cells (endothelial and Kupffer cells) were isolated from rat liver by a combined pronase-collagenase procedure and subsequent Visotrast-370 density gradient centrifugation. The lactate dehydrogenase isoenzyme pattern of fat-storing cells was found different from that of other non-parenchymal liver cells. Fat-storing cells contain LDH-4 as the main isoenzyme and do not contain LDH-1, whereas the other non-parenchymal cells have all five lactate dehydrogenase isoenzymes, among which LDH-5 is dominating. All non-parenchymal liver cell populations contain the M-type pyruvate kinase. The alkaline phosphatase of fat-storing cells has the same electrophoretic mobility as that of the other non-parenchymal cells.
Kässner
G
G
Institute of Biochemistry, School of Medicine, Karl Marx-University, Leipzig.
Scheibe
R
R
Wenzel
K W
KW
Hofmann
E
E
D000273:Adipose Tissue / Q000166:cytology / Q000201:enzymology; D000469:Alkaline Phosphatase / Q000032:analysis*; D000818:Animals; D007527:Isoenzymes / Q000032:analysis*; D007770:L-Lactate Dehydrogenase / Q000032:analysis*; D008099:Liver / Q000166:cytology / Q000201:enzymology*; D008297:Male; D011412:Propanolamines / Q000494:pharmacology*; D011770:Pyruvate Kinase / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains
D007527:Isoenzymes; D011412:Propanolamines; C058041:B 24-76; D007770:L-Lactate Dehydrogenase; D011770:Pyruvate Kinase; D000469:Alkaline Phosphatase