title: The alpha and beta subunits of phosphorylase kinase are homologous: cDNA cloning and primary structure of the beta subunit.
abstract: We have cloned cDNA molecules encoding the beta subunit of phosphorylase kinase (ATP:phosphorylase-b phosphotransferase; EC 2.7.1.38) from rabbit fast-twitch skeletal muscle and have determined the complete primary structure of the polypeptide by a combination of peptide and DNA sequencing. In the mature beta subunit, the initial methionine is replaced by an acetyl group. The subunit is composed of 1092 amino acids and has a calculated molecular mass of 125,205 Da. Alignment of its sequence with the alpha subunit of phosphorylase kinase reveals extensive regions of homology, but each molecule also possesses unique sequences. Two of the three phosphorylation sites known for the beta subunit and all seven phosphorylation sites known for the alpha subunit are located in these unique domains.
authors.affiliation: Institut für Physiologische Chemie, Ruhr-Universität Bochum, Federal Republic of Germany.
authors.forename: M W
authors.initials: MW
authors.lastname: Kilimann
authors.forename: N F
authors.initials: NF
authors.lastname: Zander
authors.forename: C C
authors.initials: CC
authors.lastname: Kuhn
authors.forename: J W
authors.initials: JW
authors.lastname: Crabb
authors.forename: H E
authors.initials: HE
authors.lastname: Meyer
authors.forename: L M
authors.initials: LM
authors.lastname: Heilmeyer
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002147:Calmodulin / Q000378:metabolism; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis*; D046911:Macromolecular Substances; D008715:Methionine; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010764:Phosphorylase Kinase / Q000032:analysis / Q000235:genetics*; D011499:Protein Processing, Post-Translational; D011817:Rabbits
chemical_list: D002147:Calmodulin; D046911:Macromolecular Substances; D004247:DNA; D008715:Methionine; D010764:Phosphorylase Kinase