Microheterogeneity and structures of neutral glycans present in quail ovomucoid.
Anionic chromatography of oligosaccharide-alditols obtained by alkaline hydrolysis of quail ovomucoid glycopeptides resulted in the isolation of a major neutral fraction (83%) which was separated into 14 fractions by high-performance liquid chromatography on an alkylamine silica column. The structures of nine oligosaccharide-alditols were determined. By the identification of mannobiosido-, and mannotriosido-di-N-acetylchitobiose mixed with N-acetyl-lactosaminic and oligomannosidic-types oligosaccharides, we demonstrate the high microheterogeneity of the glycans presents in the neutral fraction of this glycoprotein and suggest that ovomucoid glycosylation uses several pathways.
Alonso
J M
JM
Laboratorie de Chimie Biologique, Université des Sciences et Techniques de Lille Flandres Artois, Villeneuve d'Ascq, France.
Boulenguer
P
P
Wieruszeski
J M
JM
Leroy
Y
Y
Montreuil
J
J
Fournet
B
B
D000818:Animals; D002241:Carbohydrates / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D004527:Egg Proteins / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D009844:Oligosaccharides / Q000302:isolation & purification; D010059:Ovomucin / Q000032:analysis*; D011134:Polysaccharides / Q000032:analysis*; D011784:Quail
D002241:Carbohydrates; D004527:Egg Proteins; D009844:Oligosaccharides; D011134:Polysaccharides; D010059:Ovomucin