Halothane induced vasomotion of coronary, renal and iliac arterial rings in malignant hyperthermia susceptible swine.
Animals were identified as porcine malignant hyperthermia susceptible by halothane testing and were slaughtered at 90 kg of body weight. Coronary, renal and iliac arteries were isolated, dissected and 5 mm rings were mounted in 20 mL organ baths with modified Krebs solution maintained at 37 degrees C and oxygenated with 95% O2, 5% CO2. Halothane at 0%, 0.5%, 2% and 5% concentration was bubbled in the organ baths and mechanical responses were recorded over a period of 25 min. Halothane free arteries remained quiescent and the arteries from the halothane sensitive and from the halothane resistant groups reacted similarly. All arteries in the presence of halothane responded with an initial contraction of short duration followed by a relaxation and both phenomena occurred in a concentration-dependent fashion. The iliac artery was the most sensitive to halothane and responded to 0.5% concentration while coronary and renal arteries maintained the resting tension of 4 g. These results demonstrate that vascular smooth muscle, like skeletal muscle and unlike respiratory smooth muscle, has a direct pharmacological response to halothane. These observations led to the postulate that halothane by its transient but significant vasoconstrictive action could be a contributing factor to initiate the fulminant reactions occurring in malignant hyperthermia.
DeRoth
L
L
Laboratoire de Biomédecine comparée, Faculté de Médecine vétérinaire, Université de Montréal, Saint-Hyacinthe, Québec.
Nadeau
S
S
Héon
H
H
D000818:Animals; D003331:Coronary Vessels / Q000187:drug effects / Q000503:physiopathology; D046508:Culture Techniques; D006221:Halothane / Q000494:pharmacology*; D007083:Iliac Artery / Q000187:drug effects / Q000503:physiopathology; D008305:Malignant Hyperthermia / Q000503:physiopathology / Q000662:veterinary*; D009131:Muscle, Smooth, Vascular / Q000187:drug effects*; D012077:Renal Artery / Q000187:drug effects / Q000503:physiopathology; D013552:Swine; D013553:Swine Diseases / Q000503:physiopathology*; D014666:Vasomotor System / Q000187:drug effects*
D006221:Halothane