Seventy-kilodalton heat shock proteins and an additional component from reticulocyte lysate stimulate import of M13 procoat protein into microsomes.
Processing of M13 procoat protein, synthesized in a bacterial cell-free extract, to transmembrane coat protein by dog pancreas microsomes is stimulated by a system which is present in rabbit reticulocytes and depends on nucleoside triphosphates. This system consists of (at least) two components which act synergistically: members of the 70-kd heat shock protein family and (at least) one additional component. This component depends on ATP (or GTP) for its action.
Zimmermann
R
R
Institut für Physiologische Chemie der Universität München, FRG.
Sagstetter
M
M
Lewis
M J
MJ
Pelham
H R
HR
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002213:Capsid / Q000378:metabolism*; D036022:Capsid Proteins; D004285:Dogs; D006160:Guanosine Triphosphate / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000378:metabolism*; D008861:Microsomes / Q000378:metabolism*; D011498:Protein Precursors / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D012156:Reticulocytes / Q000378:metabolism
D036022:Capsid Proteins; D006360:Heat-Shock Proteins; D011498:Protein Precursors; C025668:coat protein, Bacteriophage M13; D006160:Guanosine Triphosphate; D000255:Adenosine Triphosphate