Rapid determination of amphotericin B levels in serum by high-performance liquid chromatography without interference by bilirubin.
A high-performance liquid chromatographic method for the determination of amphotericin B concentrations in human serum without bilirubin interference was developed and compared with a microbiological assay. The high-performance liquid chromatographic assay utilized a reversed-phase trimethyl silica column, simple sample preparation, and visible detection. Reproducibility studies yielded coefficient-of-variation ranges from 1.02 to 2.11% for within-day precision and 2.88 to 4.32% for between-day precision. The correlation coefficient with the microbiological assay was 0.984 for amphotericin B.
Hosotsubo
H
H
Central Laboratory for Clinical Investigation, Osaka University Hospital, Japan.
Takezawa
J
J
Taenaka
N
N
Hosotsubo
K
K
Yoshiya
I
I
D000666:Amphotericin B / Q000097:blood*; D001663:Bilirubin / Q000097:blood; D002851:Chromatography, High Pressure Liquid; D006801:Humans
D000666:Amphotericin B; D001663:Bilirubin