Effects of ethanol on protein metabolism in hepatocyte primary cultures from adult rat.
The effect of ethanol on protein synthesis and degradation in cultured hepatocytes from adult rat has been studied. The presence of 100 mM ethanol in the culture medium significantly decreased protein synthesis without affecting protein degradation rate. The depressing effect of ethanol on protein synthesis did not appear directly correlated with the changes in ATP level. However, an inhibition of sodium-dependent and energy-requiring systems of the plasma membrane following exposure to ethanol was observed.
Voci
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Istituto di Fisiologia Generale, Università di Genova, Italy.
Gallo
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G
Balestrero
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Fugassa
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E
D000227:Adenine Nucleotides / Q000378:metabolism; D000596:Amino Acids / Q000378:metabolism / Q000493:pharmacokinetics; D000818:Animals; D002478:Cells, Cultured; D000431:Ethanol / Q000494:pharmacology*; D008099:Liver / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D008297:Male; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
D000227:Adenine Nucleotides; D000596:Amino Acids; D011506:Proteins; D000431:Ethanol