title: The effect of glycosidases on the survival of rat erythrocytes in circulation.
abstract: Enzymatic removal of sialyl groups from mammalian erythrocytes resulted in their rapid sequestration from circulation subsequent to autologous transfusion. It has been demonstrated by many investigators that the terminal beta-D-galactosyl group, exposed on red blood cell by in vitro desialosylation, is recognized by an autoimmune anti-galactosyl IgG and/or by a lectin-like receptor on monocytes and macrophages. It is demonstrated herein that the disaccharide structure beta-D-Galp-(1----3)-D-GalpNAc (a) is masked in normal rat RBC, but exposed in asialo-RBC; (b) could be detected with fluorescently-labeled peanut agglutinin; (c) could be released from the asialo-RBC with an endo-N-acetyl-alpha-D-galactosaminidase; and (d) upon its removal by treatment with the endo-N-acetyl-alpha-D-galactosaminidase, enhances the survival of the asialo-RBC in circulation.
authors.affiliation: Institute of Gerontology, University of Michigan, Ann Arbor 48109.
authors.forename: K A
authors.initials: KA
authors.lastname: Gutowski
authors.forename: D A
authors.initials: DA
authors.lastname: Linseman
authors.forename: D
authors.initials: D
authors.lastname: Aminoff
mesh_terms: D000818:Animals; D004905:Erythrocyte Aging; D004912:Erythrocytes / Q000166:cytology / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000097:blood*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D006026:Glycoside Hydrolases