Phosphohistidine is found in basic nuclear proteins of Physarum polycephalum.
Nuclear extracts of the true slime mold, Physarum polycephalum, show protein histidine kinase activity towards exogenous histones [(1985) J. Biol. Chem. 260, 16106-16113]. Physarum microplasmodia were labeled with [32P]phosphate in vivo and two basic proteins containing alkali-stable phosphate were detected. The labeled proteins comigrated with Physarum histones H1 (approximately) and H2A and phosphoamino acid analysis showed that each protein contained [32P]-phosphohistidine. The H2A-like protein was also labeled in isolated nuclei incubated with [35S]thio-ATP. We conclude that some Physarum nuclear proteins contain phosphohistidine.
Pesis
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KH
Department of Biological Chemistry, University of California, Davis 95616.
Wei
Y
Y
Lewis
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M
Matthews
H R
HR
D006639:Histidine / Q000031:analogs & derivatives* / Q000032:analysis; D006657:Histones / Q000302:isolation & purification; D009687:Nuclear Proteins / Q000302:isolation & purification; D010766:Phosphorylation; D010804:Physarum / Q000032:analysis*
D006657:Histones; D009687:Nuclear Proteins; D006639:Histidine; C005436:phosphohistidine