title: Cloning and sequence analysis of cDNA for corticotropin-releasing factor precursor from the teleost fish Catostomus commersoni.
abstract: The sequence of a cDNA encoding the corticotropin-releasing factor precursor has been identified by screening lambda gt11 libraries constructed from poly(A)+ RNA of the hypothalamic region of the white sucker Catostomus commersoni brain with synthetic oligonucleotide probes deduced from the sequence of the rat corticotropin-releasing factor. The amino acid sequence of corticotropin-releasing factor of the sucker is strikingly conserved when compared to its counterpart from rat and differs only in two positions at the carboxyl terminus; in contrast, there is little similarity between their cryptic regions.
authors.affiliation: Institut für Zellbiochemie und klinische Neurobiologie, Universitäts-Krankenhaus Eppendorf, Hamburg, Federal Republic of Germany.
authors.forename: Y
authors.initials: Y
authors.lastname: Okawara
authors.forename: S D
authors.initials: SD
authors.lastname: Morley
authors.forename: L O
authors.initials: LO
authors.lastname: Burzio
authors.forename: H
authors.initials: H
authors.lastname: Zwiers
authors.forename: K
authors.initials: K
authors.lastname: Lederis
authors.forename: D
authors.initials: D
authors.lastname: Richter
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003062:Codon; D004247:DNA / Q000235:genetics*; D005399:Fishes / Q000235:genetics*; D005796:Genes; D008969:Molecular Sequence Data; D015183:Restriction Mapping
chemical_list: D003062:Codon; D004247:DNA