title: Accumulation of polymorphonuclear leukocytes in reperfused ischemic canine myocardium: relation with tissue viability assessed by fluorine-18-2-deoxyglucose uptake.
abstract: Polymorphonuclear leukocytes may participate in reperfusion injury. Whether leukocytes affect viable or only irreversibly injured tissue is not known. Therefore, we assessed the accumulation of 111In-labeled leukocytes in tissue samples characterized as either ischemic but viable or necrotic by metabolic, histochemical, and ultrastructural criteria. Six open-chest dogs received left anterior descending coronary occlusion for 2 hr followed by 4 hr reperfusion. Myocardial blood flow was determined by microspheres and autologous 111In-labeled leukocytes were injected intravenously. Fluorine-18-2-deoxyglucose, a tracer of exogenous glucose utilization, was injected 3 hr after reperfusion. The dogs were killed 4 hr after reperfusion. The risk and the necrotic regions were assessed following in vivo dye injection and postmortem tetrazolium staining. Myocardial samples were obtained in the ischemic but viable, necrotic and normal zones, and counted for 111In and 18F activity. Compared to normal, leukocytes were entrapped in necrotic regions (111In activity: 207 +/- 73%) where glucose uptake was decreased (26 +/- 15%). A persistent glucose uptake, marker of viability, was mainly seen in risk region (135 +/- 85%) where leukocytes accumulation was moderate in comparison to normal zone (146 +/- 44%). Thus, the glucose uptake observed in viable tissue is mainly related to myocytes metabolism and not to leukocytes metabolism.
authors.affiliation: Center of Nuclear Medicine, Positron Emission Tomography Laboratory, Brussels, Belgium.
authors.forename: W
authors.initials: W
authors.lastname: Wijns
authors.forename: J A
authors.initials: JA
authors.lastname: Melin
authors.forename: N
authors.initials: N
authors.lastname: Leners
authors.forename: A
authors.initials: A
authors.lastname: Ferrant
authors.forename: A
authors.initials: A
authors.lastname: Keyeux
authors.forename: J
authors.initials: J
authors.lastname: Rahier
authors.forename: M
authors.initials: M
authors.lastname: Cogneau
authors.forename: C
authors.initials: C
authors.lastname: Michel
authors.forename: A
authors.initials: A
authors.lastname: Bol
authors.forename: A
authors.initials: A
authors.lastname: Robert
mesh_terms: D000818:Animals; D003326:Coronary Circulation; D003327:Coronary Disease / Q000473:pathology / Q000628:therapy*; D003847:Deoxyglucose / Q000493:pharmacokinetics; D004285:Dogs; D005462:Fluorine Radioisotopes; D006321:Heart / Q000503:physiopathology; D007958:Leukocyte Count; D015425:Myocardial Reperfusion; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology; D009504:Neutrophils / Q000502:physiology*; D014022:Tissue Survival
chemical_list: D005462:Fluorine Radioisotopes; D003847:Deoxyglucose