Electrochemical detection for high-performance liquid chromatography of ketoconazole in plasma and saliva.
Ketoconazole, cis-1-acetyl-4-[4[[2-(2,4-dichlorophenyl)-2-(1H-imidazol- 1-ylmethyl)-1,3-dioxolan-4-yl]methoxy]phenyl]piperazine, a clinically used antifungal agent, is also an inhibitor of steroid hormone biosynthesis. A high-performance liquid chromatographic method is described which resolves ketoconazole with selectivity and high sensitivity provided by the use of electrochemical detection. Ketoconazole can be detected in high-performance liquid chromatography by electrochemical oxidation at a glassy carbon electrode at a potential of +1.0 V. Electrochemical detection offers improved sensitivity and selectivity over ultraviolet absorbance or fluorescence detection after derivatization. The method utilizes a volatile buffer system compatible with postcolumn analyses and an internal standard which is electrochemically active. This technique provides a simple method to assay ketoconazole. Ketoconazole can be detected in human plasma and saliva after a single oral therapeutic dose.
Hoffman
D W
DW
Department of Psychiatry, Dartmouth Medical School, Hanover, New Hampshire 03756.
Jones-King
K L
KL
Ravaris
C L
CL
Edkins
R D
RD
D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D007654:Ketoconazole / Q000032:analysis* / Q000097:blood; D012463:Saliva / Q000032:analysis*
D007654:Ketoconazole