Quantitative high-performance thin-layer chromatography of dansyl derivatives of biogenic amines.
A procedure for the separation, detection, and quantification of picomole levels of dansyl derivatives of the biogenic amines, dopamine, norepinephrine, octopamine, and serotonin, has been developed using high-performance thin-layer chromatography. The detection limit is 1 to 2 pmol. Each of the amine derivatives has been detected in insect brain tissue and a solvent system has been developed for the separation and quantification of octopamine in insect tissue samples.
Eaton
J L
JL
Department of Entomology, Virginia Polytechnic Institute and State University, Blacksburg 24061.
Mullins
D E
DE
D000818:Animals; D001679:Biogenic Amines / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D003619:Dansyl Compounds; D005216:Fat Body / Q000032:analysis; D010526:Periplaneta / Q000032:analysis
D001679:Biogenic Amines; D003619:Dansyl Compounds