DNA adducts formed by the comutagens harman and norharman in various tissues of mice.
Covalent modifications of DNA in various tissues of mice with harman or norharman were analyzed by 32P-postlabeling assay. Administration of 0.1% harman to mice in their diet for 4 weeks resulted in DNA adducts in the liver and kidney. No specific DNA adduct was detected in other tissues, such as the glandular stomach, large intestine and brain. Similar treatment of mice with norharman resulted in DNA adducts in the kidney, glandular stomach and large intestine, but not in the liver or brain. These results suggests the in vivo genotoxicities of harman and norharman.
Yamashita
K
K
National Cancer Center Research Institute, Tokyo, Japan.
Ohgaki
H
H
Wakabayashi
K
K
Nagao
M
M
Sugimura
T
T
D000470:Alkaloids; D000818:Animals; D002243:Carbolines; D004247:DNA / Q000378:metabolism; D004249:DNA Damage; D005753:Gastric Mucosa / Q000378:metabolism; D006247:Harmine / Q000031:analogs & derivatives; D007420:Intestine, Large / Q000378:metabolism; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D051379:Mice; D014018:Tissue Distribution
D000470:Alkaloids; D002243:Carbolines; D006247:Harmine; C005010:harman; D004247:DNA; C010262:norharman