Alkylation of protein and DNA in mice treated with urethane.
14C-labelled urethane in the dose interval 1-260 mg/kg was given to male CBA mice by i.p. injection. The formation of epoxyethyl carbamate, the suggested electrophilic intermediate of urethane, was studied through measurements of 2-oxoethyl adducts to the amino acids cysteine, terminal valine and histidine in protein (especially haemoglobin) and to N-7 of guanine in DNA in various tissues 9 h after treatment. The extent of binding to haemoglobin and liver DNA increased linearly with administered amount, in accordance with a complete clearance of urethane at all doses. The DNA binding varied between organs and decreased in the order liver greater than kidney greater than spleen greater than testis.
Svensson
K
K
Department of Radiobiology, Stockholm University, Sweden.
D000478:Alkylation; D000818:Animals; D002273:Carcinogens / Q000378:metabolism*; D004247:DNA / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D006454:Hemoglobins / Q000378:metabolism; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D011506:Proteins / Q000378:metabolism*; D012306:Risk; D014520:Urethane / Q000378:metabolism* / Q000633:toxicity; D014752:Vinyl Chloride / Q000633:toxicity
D002273:Carcinogens; D006454:Hemoglobins; D011506:Proteins; D014520:Urethane; D004247:DNA; D014752:Vinyl Chloride