title: Hypophysectomy and adrenalectomy decrease Ca2+-induced swelling of rat liver mitochondria.
abstract: Hypophysectomized rats showed significant modifications in Ca2+-induced swelling and MgATP-induced contraction of liver mitochondria. Two-three months after operation, maximal swelling (MS), rate of swelling (VS), maximal contraction (MC) and rate of contraction (VC) were decreased by 77 (MS), 83 (VS), 79 (MC) and 62 (VC) % (in all cases P less than 0.05 as compared with normal rat mitochondria). Treatment of hypophysectomized rats with bovine pituitary extract, for two weeks, counteracted the effect of hypophysectomy, whereas treatment with bovine growth hormone was ineffective. Adrenalectomy produced similar though less extensive modification of Ca2+-induced swelling but failed to affect the MgATP-dependent mitochondrial contraction. Substrate oxidation by hypophysectomized or adrenalectomized rat mitochondria showed no modification of the energy-yielding mechanisms, capable of explaining the observed changes in mitochondrial swelling.
authors.affiliation: Instituto de Química Biológica, Facultad de Medicina, Universidad de Buenos Aires, Argentina.
authors.forename: C D
authors.initials: CD
authors.lastname: Bedetti
authors.forename: L F
authors.initials: LF
authors.lastname: Pacheco Bolaños
authors.forename: G
authors.initials: G
authors.lastname: Anzola Montero
authors.forename: A O
authors.initials: AO
authors.lastname: Stoppani
mesh_terms: D000311:Adrenal Glands / Q000502:physiology*; D000315:Adrenalectomy; D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D007016:Hypophysectomy; D008930:Mitochondria, Liver / Q000187:drug effects*; D008933:Mitochondrial Swelling / Q000187:drug effects*; D010101:Oxygen Consumption / Q000187:drug effects; D010902:Pituitary Gland / Q000502:physiology*; D051381:Rats; D014020:Tissue Extracts / Q000494:pharmacology
chemical_list: D014020:Tissue Extracts; D002118:Calcium