Gallidermin: a new lanthionine-containing polypeptide antibiotic.
Gallidermin is a new member of the class of lanthionine-containing peptide antibiotics, which are summarized under the common name lantibiotics. The lantibiotic gallidermin is produced by Staphylococcus gallinarum (F16/P57) Tü3928, and it exhibits activities against the Propionibacteria, involved in acne disease. Gallidermin differs from the recently discovered tetracyclic 21-residue peptide antibiotic epidermin only in a Leu/Ile exchange in position 6. The isolation procedures for gallidermin included adsorption directly from the culture broth, ion-exchange chromatography of the amphiphilic and basic polypeptide followed by desalting, and final purification by reversed-phase HPLC. The structural elucidation of the polypeptide containing four thioether bridges involved mainly a combination of automated gas-phase sequencing, thermospray liquid chromatography/mass spectrometry and fast-atom-bombardment mass spectrometry.
Kellner
R
R
Institut für Organische Chemie, Universität Tübingen, Federal Republic of Germany.
Jung
G
G
Hörner
T
T
Zähner
H
H
Schnell
N
N
Entian
K D
KD
Götz
F
F
D000409:Alanine / Q000031:analogs & derivatives* / Q000032:analysis; D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000302:isolation & purification* / Q000494:pharmacology; D001430:Bacteriocins; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D008401:Gas Chromatography-Mass Spectrometry; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010455:Peptides; D010456:Peptides, Cyclic / Q000302:isolation & purification / Q000494:pharmacology; D013210:Staphylococcus / Q000378:metabolism; D013440:Sulfides
D000900:Anti-Bacterial Agents; D001430:Bacteriocins; D010455:Peptides; D010456:Peptides, Cyclic; D013440:Sulfides; C057656:gallidermin; C050558:epidermin; C001520:lanthionine; D000409:Alanine