Development of polyclonal and monoclonal antibodies to iodinated contrast media.
Two independent systems were developed to produce antibodies to diatrizoate. In the rabbit model polyclonal antibodies were produced that showed cross-reactivity to certain other contrast agents, analogues and serum proteins. In the mouse model monoclonal antibodies were produced that reacted only with diatrizoate, metrizamide, and ovalbumin. These studies demonstrated the potential antigenicity of contrast medium.
Siegle
R L
RL
Department of Radiology, University of Texas Health Science Center, San Antonio 78284.
McGuire
W L
WL
Peters
J E
JE
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000917:Antibody Formation / Q000187:drug effects*; D003287:Contrast Media / Q000276:immunology*; D005260:Female; D007463:Iodobenzoates / Q000276:immunology*; D008722:Methods; D051379:Mice; D008807:Mice, Inbred BALB C; D011817:Rabbits; D014283:Triiodobenzoic Acids / Q000276:immunology*
D000911:Antibodies, Monoclonal; D003287:Contrast Media; D007463:Iodobenzoates; D014283:Triiodobenzoic Acids