Dermal toxicity of a high-boiling (bp 250-450 degrees C) coal liquefaction product in the rat--II.
Coal liquefaction products have been considered as an alternate source of energy to replace conventional crude oil. The present study was designed to investigate the dermal toxicity of a heavy fraction of coal liquefaction product (CLP, bp 250-450 degrees C) in the rat. Groups of 10 male and 10 female Sprague-Dawley rats (180-200 g) were treated dermally with CLP at dose levels of 100, 200, 400, or 800 mg/kg body weight.d for 6 wk. The controls were treated with 0.4 ml/kg of normal saline, while the positive control group received 400 mg/kg diesel fuel. Growth suppression was observed in all CLP-treated groups of males; in the females this effect occurred in the two highest dose groups. Diesel fuel at 400 mg/kg also caused growth suppression of a similar magnitude to that of CLP in male rats. Male animals treated with high doses of CLP or diesel fuel had severe skin lesions. Increased liver weights were observed in the diesel fuel-treated as well as all CLP-treated groups of females. The kidney weights of females treated with 400 mg/kg and 800 mg/kg CLP were also higher than control values. Decreased red cell count, hemoglobin, and hematocrit volume occurred in some CLP and diesel fuel groups of both sexes. There was mild bone marrow hyperplasia in rats of both sexes treated with CLP or diesel fuel. Mild histological changes were observed in the thyroid, liver, bone marrow, and skin of rats of both sexes treated with CLP and diesel fuel. Based on the data presented, dermal application of CLP produced systemic toxicity at the dose levels studied, and CLP and diesel fuel possess toxic effects of similar nature and magnitude.
Chu
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Bureau of Chemical Hazards, Environmental Health Directorate, Ottawa, Ontario, Canada.
Villeneuve
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DC
Côté
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M
Secours
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V
Otson
R
R
Valli
V E
VE
D000818:Animals; D001773:Blood Cells / Q000187:drug effects; D003031:Coal; D004305:Dose-Response Relationship, Drug; D005260:Female; D006128:Growth / Q000187:drug effects*; D008297:Male; D009929:Organ Size / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains; D012867:Skin / Q000187:drug effects* / Q000473:pathology
D003031:Coal