The effect of cyclosporin A on the growth and prolactin binding to Nb-2 rat lymphoma cells.
Cyclosporin A, an immunosuppressive agent, inhibited the prolactin stimulated growth of rat lymphoma Nb-2 cells. In the presence of 1 ng/ml of prolactin, 50% inhibition of growth was at 5 x 10(-6) M and the inhibition was reversible. The Kd of cyclosporin A binding to the Nb-2 cells was 10(-7) M and was independent of prolactin. The Kd of prolactin binding to the Nb-2 cells was 2 x 10(-10) M. Cyclosporin A did not influence the binding of prolactin to the cells and vice versa. The inhibitory effect of cyclosporin A on the growth of Nb-2 cells is due to some step other than the binding of prolactin to the cells.
Varma
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Department of Biochemistry & Molecular Biology, University of Kansas Medical Center, Kansas City 66103.
Ebner
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KE
D000818:Animals; D001667:Binding, Competitive; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D003524:Cyclosporins / Q000378:metabolism / Q000494:pharmacology*; D007700:Kinetics; D008223:Lymphoma / Q000473:pathology*; D011388:Prolactin / Q000378:metabolism / Q000494:pharmacology; D051381:Rats; D011981:Receptors, Prolactin / Q000378:metabolism*
D003524:Cyclosporins; D011981:Receptors, Prolactin; D011388:Prolactin