title: Global cerebral ischemia and intracellular pH during hyperglycemia and hypoglycemia in cats.
abstract: In 27 cats treated to vary arterial serum glucose concentrations, we measured cerebral high-energy phosphate metabolite concentration and intracellular pH using in vivo phosphorus-31 nuclear magnetic resonance spectroscopy during transient global cerebral ischemia and reperfusion. Hypoglycemia was induced with 4 units/kg i.v. insulin in six cats before ischemia; hyperglycemia was induced with 1.5 g/kg i.v. glucose in six cats before and in six cats during ischemia. Nine untreated cats subjected to ischemia without manipulation of blood glucose concentration served as controls. During ischemia, intracellular pH fell to similar levels in the control and both hyperglycemic groups. During reperfusion, the hyperglycemic before ischemia group initially exhibited a severe further decline in intracellular pH (p less than 0.003); this further decline was not observed in the control or the hyperglycemic during ischemia groups. Intracellular acidosis was attenuated both during ischemia and early after reperfusion in the hypoglycemic before ischemia group. In all groups, cerebral high-energy phosphate metabolite concentrations were depleted during ischemia and then recovered to the same degree during reperfusion. Our data suggest that brain glucose stores before ischemia determine the severity and time course of intracellular acidosis during ischemia and reperfusion.
authors.affiliation: Department of Neurology, Henry Ford Hospital, Detroit, MI 48202.
authors.forename: M
authors.initials: M
authors.lastname: Chopp
authors.forename: K M
authors.initials: KM
authors.lastname: Welch
authors.forename: C D
authors.initials: CD
authors.lastname: Tidwell
authors.forename: J A
authors.initials: JA
authors.lastname: Helpern
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism* / Q000648:ultrastructure; D002545:Brain Ischemia / Q000150:complications* / Q000378:metabolism / Q000628:therapy; D002415:Cats; D005260:Female; D006859:Hydrogen / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D006943:Hyperglycemia / Q000150:complications* / Q000378:metabolism; D007003:Hypoglycemia / Q000150:complications* / Q000378:metabolism; D007425:Intracellular Membranes / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D010710:Phosphates / Q000378:metabolism; D010758:Phosphorus / Q000378:metabolism; D015424:Reperfusion; D013997:Time Factors
chemical_list: D010710:Phosphates; D010758:Phosphorus; D006859:Hydrogen