title: Altered fatty acid composition in the plasma, platelets, and aorta of the streptozotocin-induced diabetic rat.
abstract: Decreased arachidonate levels have been described in various tissues of the streptozotocin-induced diabetic rat. However, reported arachidonate changes in platelets from diabetic patients have been variable. In this communication, we describe experiments that indicate that in the short-term streptozotocin diabetic rat (2 to 3 weeks), the fatty acid composition of plasma and red blood cell lipids was altered but remained unchanged in platelet and aorta phospholipids. The altered fatty acid composition of the diabetic red blood cells and plasma cholesterol esters and phospholipids was similar to that previously found in the diabetic liver. However, in long-term diabetes (6 weeks), the phospholipid fatty acid composition of the platelet and aorta became significantly altered. Thus, in the 6-week diabetic platelet, there were increases of linoleate, dihomo-gamma-linolenate, docosapentaenoate (C22:5n-3), and docosahexaenoate, and decreases of oleate, arachidonate, and docosatetraenoate. In the aorta, there were increases of linoleate, eicosapentaenoate, and docosahexaenoate, and decreases of arachidonate, docosatetraenoate, and docosapentaenoate (C22:5n-6). Results from these experiments indicate that the fatty acid composition of plasma and red blood cell lipids was altered in short-term diabetes (2 to 3 weeks), but that of platelet and aorta phospholipids was not changed until more prolonged diabetes was present. Insulin treatment of the diabetic rat increased the levels of palmitoleate and oleate and decreased the levels of linoleate in platelet and aorta lipids from insulin-treated diabetic rats, suggesting an overcorrection of diminished delta 9 and delta 6 fatty acid desaturation as compared with the nondiabetic control.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: John L. McClellan Memorial Veterans Hospital, Little Rock, AR.
authors.forename: A Q
authors.initials: AQ
authors.lastname: Dang
authors.forename: F H
authors.initials: FH
authors.lastname: Faas
authors.forename: J A
authors.initials: JA
authors.lastname: Lee
authors.forename: W J
authors.initials: WJ
authors.lastname: Carter
mesh_terms: D000818:Animals; D001011:Aorta / Q000378:metabolism*; D001792:Blood Platelets / Q000378:metabolism*; D002788:Cholesterol Esters / Q000097:blood; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D005227:Fatty Acids / Q000097:blood / Q000378:metabolism*; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000378:metabolism*; D010743:Phospholipids / Q000097:blood / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values; D014280:Triglycerides / Q000097:blood
chemical_list: D002788:Cholesterol Esters; D005227:Fatty Acids; D010743:Phospholipids; D014280:Triglycerides