Inhibition of cell growth retains differentiated function of bovine parathyroid cells in monolayer culture.
Monolayer culture of bovine parathyroid cells for up to 4 days in medium containing 10% serum resulted in cell hypertrophy and proliferation as well as functional dedifferentiation. Compared to freshly isolated cells there was a right-shifted dose-effect relationship for calcium-inhibited parathyroid hormone (PTH) release, a decreased suppressibility of secretion and an inability of the cytoplasmic Ca2+ concentration (Ca2+i) to follow changes in the extracellular Ca2+ concentration. The functional changes seemed not to be due to culture per se, since human parathyroid cells exhibited an essentially unchanged regulation of Ca2+i even after 8 days of culture in 10% serum. During culture in medium containing 0.1% serum, the bovine cells did not proliferate and there was only slight cell hypertrophy. These cells retained much of their ability to regulate Ca2+i and PTH release. The results indicate that functional dedifferentiation of parathyroid cells is related to cell growth.
Nygren
P
P
Department of Medical Cell Biology, University of Uppsala, Sweden.
Larsson
R
R
Johansson
H
H
Gylfe
E
E
Rastad
J
J
Akerström
G
G
D000818:Animals; D001769:Blood; D002118:Calcium / Q000494:pharmacology*; D002417:Cattle; D002454:Cell Differentiation; D002455:Cell Division; D002478:Cells, Cultured; D003470:Culture Media; D004247:DNA / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D010280:Parathyroid Glands / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D010281:Parathyroid Hormone / Q000378:metabolism*
D003470:Culture Media; D010281:Parathyroid Hormone; D004247:DNA; D002118:Calcium