title: Determination of nitrilotriacetate in biological matrices using ion exclusion chromatography.
abstract: A sensitive method for the determination of nitrilotriacetate in biological growth media and cell-free extracts by ion exclusion chromatography is described using HCl as an eluant. The eluant conductivity was chemically suppressed with a membrane suppressor and a conductivity detector was used for subsequent detection. The membrane was continuously regenerated with a tetrabutylammoniumhydroxide solution. The detection limit for nitrilotriacetate in cell-free extract was 11 mg/liter, while for nitrilotriacetate in growth media it was 1 mg/liter. Interference by compounds present in biological matrices with the determination is discussed.
authors.affiliation: Institute of Aquatic Sciences, Swiss Federal Institute of Technology, Zürich.
authors.forename: R P
authors.initials: RP
authors.lastname: Schneider
authors.forename: F
authors.initials: F
authors.lastname: Zürcher
authors.forename: T
authors.initials: T
authors.lastname: Egli
authors.forename: G
authors.initials: G
authors.lastname: Hamer
mesh_terms: D000085:Acetates / Q000032:analysis*; D002138:Calibration; D002474:Cell-Free System; D002845:Chromatography / Q000295:instrumentation; D003470:Culture Media; D009571:Nitrilotriacetic Acid / Q000032:analysis*; D011549:Pseudomonas
chemical_list: D000085:Acetates; D003470:Culture Media; D009571:Nitrilotriacetic Acid