[A study of various properties of beta-galactocerebrosidase from human chorion using synthetic fluorescent substrates].
The properties of beta-galactocerebrosidase from human chorionic villi, cultured chorionic villi and cultured skin fibroblasts were compared, using 6-hexadecanoylamino-4-methylumbelliferyl-beta-D-galactopyranoside (HMGaL) as substrate. The effects of bile salt and Triton X-100 on beta-galactocerebrosidase were examined. It was shown that optimization of the HMGaL assay system requires the presence of pure sodium taurocholate and Triton X-100 at concentrations of 4.5 mM and 0.28 mM, respectively. The optimal pH value was found to be equal to 4.5-5.0; Km for the substrate was 0.03 mM. A comparison of beta-galactocerebrosidase from chorionic villi and cultured chorionic villi with the enzyme from skin fibroblasts revealed the similarity of some properties of these enzymes. The experimental results suggest that HMGaL can be used as a substrate for the identification of chorionic villi beta-galactocerebrosidase in an early prenatal diagnosis of Krabbe's disease.
Petushkova
N A
NA
Kozlova
I K
IK
Tsvetkova
I V
IV
Vidershaĭn
G Ia
GIa
D002478:Cells, Cultured; D002823:Chorion / Q000201:enzymology*; D005347:Fibroblasts / Q000201:enzymology; D005456:Fluorescent Dyes; D005696:Galactosidases / Q000032:analysis*; D005697:Galactosides; D005698:Galactosylceramidase / Q000032:analysis*; D006027:Glycosides; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006923:Hymecromone / Q000031:analogs & derivatives; D007700:Kinetics; D007965:Leukodystrophy, Globoid Cell / Q000175:diagnosis; D013379:Substrate Specificity; D014468:Umbelliferones
D005456:Fluorescent Dyes; D005697:Galactosides; D006027:Glycosides; D014468:Umbelliferones; D006923:Hymecromone; C047480:6-hexadecanoylamino-4-methylumbelliferyl-galactopyranoside; D005696:Galactosidases; D005698:Galactosylceramidase
Izuchenie nekotorykh svoĭstv beta-galaktotserebrozidazy khoriona cheloveka s pomoshch'iu sinteticheskogo fluorogennogo substrata.