title: Molecular structure of serum transferrin at 3.3-A resolution.
abstract: Serum transferrin is a metal-binding glycoprotein, molecular weight ca. 80,000, whose primary function is the transport of iron in the plasma of vertebrates. The X-ray crystallographic structure of diferric rabbit serum transferrin has been determined to a resolution of 3.3 A. The molecule has a beta alpha structure of similar topology to human lactoferrin and is composed of two homologous lobes that each bind a single ferric ion. Each lobe is further divided into two dissimilar domains, and the iron-binding site is located within the interdomain cleft. The iron is bound by two tyrosines, a histidine, and an aspartic acid residue. The location of the 19 disulfide bridges is described, and their possible structural roles are discussed in relation to the transferrin family of proteins. Mapping of the intron/exon splice junctions onto the molecule provides some topological evidence in support of the putative secondary role for transferrin in stimulating cell proliferation.
authors.affiliation: Department of Crystallography, Birkbeck College, University of London, U.K.
authors.forename: S
authors.initials: S
authors.lastname: Bailey
authors.forename: R W
authors.initials: RW
authors.lastname: Evans
authors.forename: R C
authors.initials: RC
authors.lastname: Garratt
authors.forename: B
authors.initials: B
authors.lastname: Gorinsky
authors.forename: S
authors.initials: S
authors.lastname: Hasnain
authors.forename: C
authors.initials: C
authors.lastname: Horsburgh
authors.forename: H
authors.initials: H
authors.lastname: Jhoti
authors.forename: P F
authors.initials: PF
authors.lastname: Lindley
authors.forename: A
authors.initials: A
authors.lastname: Mydin
authors.forename: R
authors.initials: R
authors.lastname: Sarra
mesh_terms: D000818:Animals; D004220:Disulfides; D005091:Exons; D006023:Glycoproteins; D007501:Iron; D008958:Models, Molecular; D011487:Protein Conformation; D011817:Rabbits; D014168:Transferrin / Q000235:genetics; D014961:X-Ray Diffraction
chemical_list: D004220:Disulfides; D006023:Glycoproteins; D014168:Transferrin; D007501:Iron