Nonhistone nuclear proteins specific to certain mouse embryonal carcinoma clones having an inactive X chromosome.
By means of one-dimensional polyacrylamide slab gel electrophoresis nonhistone nuclear proteins were compared in murine embryonal carcinoma cell clones with two X chromosomes; both are active in some clones and one of them is inactive in others and in a population of cells having only one X chromosome. Under our experimental conditions, we succeeded in finding two extra bands at approximately 46,000 Da in cells having an inactive X chromosome. Furthermore, a band at approximately 71,000 Da was significantly heavier in cells having an inactive X chromosome than in those having two active X or those having only one X chromosome.
Abe
K
K
Chromosome Research Unit, Faculty of Science, Hokkaido University, Sapporo, Japan.
Takagi
N
N
Sasaki
M
M
D000818:Animals; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D051379:Mice; D008970:Molecular Weight; D013724:Teratoma / Q000235:genetics*; D014960:X Chromosome
D002868:Chromosomal Proteins, Non-Histone