Sulfogalactolipid binding protein SLIP 1: a conserved function for a conserved protein.
We have studied the species and tissue expression of the 68kD sulfogalactolipid binding protein SLIP 1, originally detected in the male germ cells of the rat (Lingwood: Can. J. Biochem. Cell Biol., 63:1077-1085, 1985). Our results show that SLIP 1 has been highly conserved during evolution and is found in the testes of all vertebrates tested. In studies in the rat, we have found that SLIP 1 is, however, tissue restricted, being found only in the brain (also a major site of sulfogalactolipid biosynthesis) in addition to the testis. SLIP 1 was also detected in mammalian oocytes. The SLIP 1 species detected in brain and oocytes retain the sulfogalactolipid-binding characteristics of rat testicular SLIP 1, indicating that, in addition to immunological features, the glycolipid-binding function of SLIP 1 is conserved in these tissues.
Law
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Department of Biochemistry, Hospital for Sick Children, Toronto, Ontario, Canada.
Itkonnen
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O
Lingwood
C A
CA
D000818:Animals; D005075:Biological Evolution; D001923:Brain Chemistry; D002352:Carrier Proteins / Q000032:analysis* / Q000378:metabolism; D018797:Cell Cycle Proteins; D005260:Female; D006017:Glycolipids / Q000378:metabolism*; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000032:analysis* / Q000378:metabolism; D009865:Oocytes / Q000032:analysis; D016601:RNA-Binding Proteins; D051381:Rats; D011919:Rats, Inbred Strains; D013737:Testis / Q000032:analysis*; D014714:Vertebrates / Q000378:metabolism*
D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D006017:Glycolipids; C489104:MIF4GD protein, human; D008565:Membrane Proteins; D016601:RNA-Binding Proteins; C051337:Slip1 protein, rat