title: Morphological changes in spinal motor neurons giving rise to long-term regenerated sciatic nerve axons.
abstract: Morphological properties of rat spinal motor neurons were examined 14-16 months following unilateral sciatic nerve crush and compared to the properties observed in neurons contralateral to injury and in cord segments from age-matched control rats. Regenerated and control motor neurons were identified by retrograde labelling with HRP applied to sciatic nerves distal to the site of crush or at a comparable location in control nerves. Many of the experimental motor neurons were enlarged and had thickened dendritic processess compared to the finer dendrites seen in control cells. Mean cell area ipsilateral to the crush lesions was larger than mean control cell area (P-value less than 0.001) despite representation of all control cell areas in the regenerated population. These data suggest that persistent or continued morphological abnormalities occur in mammalian motor neurons following simple sciatic crush injury when examined at extended times beyond the period of axonal regeneration and clinical recovery.
authors.affiliation: Department of Pediatrics, Brown University, Providence, RI 02912.
authors.forename: C M
authors.initials: CM
authors.lastname: Bowe
authors.forename: C H
authors.initials: CH
authors.lastname: Yu
authors.forename: S G
authors.initials: SG
authors.lastname: Waxman
mesh_terms: D000818:Animals; D001369:Axons / Q000502:physiology; D006735:Horseradish Peroxidase; D009046:Motor Neurons / Q000166:cytology / Q000502:physiology*; D009409:Nerve Crush; D009416:Nerve Regeneration; D051381:Rats; D011919:Rats, Inbred Strains; D012584:Sciatic Nerve / Q000166:cytology / Q000502:physiology*; D013116:Spinal Cord / Q000166:cytology / Q000502:physiology*; D013997:Time Factors
chemical_list: D006735:Horseradish Peroxidase