Lipid detection by malachite green-aldehyde in the dental basement membrane in the rat incisor.
Developing rat incisors were treated with malachite green-aldehyde fixative solution (MGA), which retains and stains lipids. We observed positive staining occurring as dots in the basement membrane. Most of these dots (2-3.5 nm in diameter) were grouped in the lamina densa but some were also present in the lamina lucida and the lamina fibroreticularis. These data provide evidence for the existence of lipids in the dental basement membrane and suggest that they are distributed together with the various groups of proteins so far detected.
Goldberg
M
M
Laboratoire d'Histologie, Faculté de Chirurgie Dentaire, Université René Descartes PARIS V, Montrouge, France.
Lecolle
S
S
Ruch
J V
JV
Staubli
A
A
Septier
D
D
D000447:Aldehydes; D000818:Animals; D001485:Basement Membrane / Q000032:analysis / Q000648:ultrastructure; D007180:Incisor / Q000032:analysis*; D008055:Lipids / Q000032:analysis*; D008854:Microscopy, Electron; D051381:Rats; D011919:Rats, Inbred Strains; D012394:Rosaniline Dyes
D000447:Aldehydes; D008055:Lipids; D012394:Rosaniline Dyes; C005095:malachite green