Early events in inositol phosphate metabolism in longitudinal smooth muscle from guinea-pig intestine stimulated with carbachol.
In longitudinal smooth muscle from guinea-pig intestine prelabelled with [3H]inositol, carbachol produced a 3-fold increase in [3H]inositol 1,4,5-trisphosphate within 2 s, and there was also a simultaneous increase in [3H]inositol 1,4-bisphosphate. 3H-labelling of inositol 1,3,4,5-tetrakisphosphate was not significantly increased until 60 s after carbachol stimulation, and the accumulation of [3H]inositol 1,3,4-trisphosphate was relatively small.
Salmon
D M
DM
Department of Pharmacology, St. George's Hospital Medical School, London, U.K.
Bolton
T B
TB
D000818:Animals; D002217:Carbachol / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006168:Guinea Pigs; D066298:In Vitro Techniques; D007295:Inositol Phosphates / Q000378:metabolism*; D009130:Muscle, Smooth / Q000187:drug effects / Q000378:metabolism*; D013268:Stimulation, Chemical; D013403:Sugar Phosphates / Q000378:metabolism*; D014316:Tritium
D007295:Inositol Phosphates; D013403:Sugar Phosphates; D014316:Tritium; C081655:inositol-1,3,4,5-tetrakisphosphate; D002217:Carbachol