Studies on the biosynthesis of the antibiotic crisamicin A and carbon-13 magnetic resonance assignments.
The biosynthesis of crisamicin A, a novel dimeric isochromanequinone antibiotic from Micromonospora purpureochromogenes subsp. halotolerans has been investigated by [1-13C] and [2-13C] labeled acetate precursor feeding experiments. Analysis of the proton noise decoupled and off resonance 13C NMR spectra of 13C enriched and unenriched crisamicin A and their acetate derivatives indicated the biosynthesis via the polyketide pathway, as expected. Further analysis of the enriched spectra allowed the complete assignment of the carbon signals. Of particular interest was the establishment of the linkage between the two monomeric halves of the molecule and determination of the location of the phenolic hydroxyls.
Nelson
R A
RA
Waksman Institute of Microbiology, Rutgers State University of New Jersey, Piscataway 08855-0759.
Pope
J A
JA
Pandey
R C
RC
McDaniel
L E
LE
Schaffner
C P
CP
Beveridge
R L
RL
Hoops
P H
PH
Jordan
F
F
D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D009682:Magnetic Resonance Spectroscopy; D009285:Naphthoquinones / Q000096:biosynthesis
D000900:Anti-Bacterial Agents; D009285:Naphthoquinones; C048652:crisamicin A