Hemoglobin: a lifesaver and an oxidant. How to tip the balance.
Hemoglobin solutions were assessed in terms of their ability to promote lipid peroxidation, which was quantitated by measuring the formation of thiobarbituric acid reactive substances (TBARS) under specified conditions in murine brain homogenates. Solutions designed for use in acute treatment of hypovolemic shock and trauma should incorporate ingredients specifically aimed at decreasing oxygen and lipid radical mediated injury occurring secondary to ischemia and reperfusion. A number of strategies aimed at decreasing the oxidant effect of hemoglobin solutions and other blood and plasma substitutes have been evaluated. These include use of the naturally occurring anti-oxidants in human plasma, specifically transferrin and ceruloplasmin. Similarly, certain iron chelators, such as deferoxamine (Desferal, Ciba-Geigy), effectively prevent molecular and cellular damage caused by iron catalyzed formation of oxygen derived radicals.
Faassen
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Dight Laboratories, University of Minnesota, Minneapolis 55455.
Sundby
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Panter
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Condie
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Hedlund
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D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D002263:Carboxyhemoglobin / Q000494:pharmacology; D002570:Ceruloplasmin / Q000494:pharmacology; D003676:Deferoxamine / Q000494:pharmacology; D006454:Hemoglobins / Q000302:isolation & purification / Q000494:pharmacology*; D008054:Lipid Peroxides / Q000378:metabolism*; D008706:Methemoglobin / Q000494:pharmacology; D051379:Mice; D010108:Oxyhemoglobins / Q000494:pharmacology; D012709:Serum Albumin / Q000494:pharmacology; D012996:Solutions; D013858:Thiobarbiturates; D014168:Transferrin / Q000494:pharmacology
D006454:Hemoglobins; D008054:Lipid Peroxides; D010108:Oxyhemoglobins; D012709:Serum Albumin; D012996:Solutions; D013858:Thiobarbiturates; D014168:Transferrin; D008706:Methemoglobin; D002263:Carboxyhemoglobin; D002570:Ceruloplasmin; D003676:Deferoxamine; C029684:thiobarbituric acid