title: Rapid isolation of benzodiazepines with Sep-Pak C18 cartridges.
abstract: A rapid method for isolation of various benzodiazepines from human samples with Sep-Pak C18 cartridges before wide-bore capillary gas chromatography is described. The drugs dissolved in alkaline samples were directly applied to the cartridges and eluted with hexane/isopropanol (9:1). The recoveries were excellent for all drugs in urine samples, but were somewhat lower for some drugs in plasma samples. The latter problem could be easily circumvented by using a deproteinization process before their application to the cartridge. We can recommend the Sep-Pak C18 cartridges for isolation of benzodiazepines because of their simplicity and rapidity.
authors.affiliation: Department of Legal Medicine, Hamamatsu University School of Medicine, Japan.
authors.forename: O
authors.initials: O
authors.lastname: Suzuki
authors.forename: H
authors.initials: H
authors.lastname: Seno
authors.forename: T
authors.initials: T
authors.lastname: Kumazawa
mesh_terms: D001569:Benzodiazepines / Q000032:analysis* / Q000097:blood / Q000652:urine; D002849:Chromatography, Gas; D006801:Humans
chemical_list: D001569:Benzodiazepines