Quantitation of adriamycin content by a sensitive immunochemical assay.
An immunoenzymatic method for the quantitation of adriamycin (ADM) content in tissues as well as in tumor cells has been developed. This procedure has three main advantages. Firstly, it is possible to carry out the determination on whole homogenates and blood serum, thus avoiding the extractive procedures. Secondly, very low ADM concentrations (0.2 ng) can be detected. Thirdly, it is possible to determine simultaneously and in triplicate both the standard curve and ADM concentration in twelve different samples with a great reduction of the experimental variability.
Citro
G
G
Regina Elena Institute for Cancer Research, Rome, Italy.
Verdina
A
A
Galati
R
R
Floridi
A
A
D000818:Animals; D000936:Antigen-Antibody Complex / Q000032:analysis; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism; D004317:Doxorubicin / Q000032:analysis* / Q000276:immunology / Q000493:pharmacokinetics; D004797:Enzyme-Linked Immunosorbent Assay; D007106:Immune Sera; D007074:Immunoglobulin G; D008297:Male; D051379:Mice; D051381:Rats; D011919:Rats, Inbred Strains; D013053:Spectrophotometry / Q000379:methods; D014018:Tissue Distribution
D000936:Antigen-Antibody Complex; D007106:Immune Sera; D007074:Immunoglobulin G; D004317:Doxorubicin