title: Nuclear oncogene amplification or rearrangement is not involved in human colorectal malignancies.
abstract: We have examined 44 cases of human colonic and rectal carcinomas for structural rearrangement and amplification of c-myc, N-myc, L-myc, c-myb and p53 oncogenes. DNA hybridization showed evidence of c-myc amplification in only one of the samples tested. In addition, the same tumour also showed a rearrangement immediately 3' to the c-myc locus. No rearrangement could be found at the c-myc locus in the other 43 cases. Moreover, our molecular analysis of N-myc, L-myc, c-myb and p53 genes indicated no relevant alteration of the copy number and/or genomic structure of these nuclear oncogenes. Thus, at least in human colorectal malignancies, it is unlikely that nuclear oncogene structural alterations and/or amplification plays a major role in tumour induction or progression.
authors.affiliation: Centro di Riferimento Oncologico, Aviano (Pordenone), Italy.
authors.forename: R
authors.initials: R
authors.lastname: Dolcetti
authors.forename: V
authors.initials: V
authors.lastname: De Re
authors.forename: A
authors.initials: A
authors.lastname: Viel
authors.forename: M
authors.initials: M
authors.lastname: Pistello
authors.forename: M
authors.initials: M
authors.lastname: Tavian
authors.forename: M
authors.initials: M
authors.lastname: Boiocchi
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics*; D003110:Colonic Neoplasms / Q000235:genetics*; D004247:DNA; D005784:Gene Amplification; D015321:Gene Rearrangement; D006801:Humans; D009693:Nucleic Acid Hybridization; D009857:Oncogenes; D012004:Rectal Neoplasms / Q000235:genetics*
chemical_list: D004247:DNA