A general reagent for amplifying ELISAs.
This paper describes a method to enhance the sensitivity of enzyme immunoassays by the use of solid tagged latex beads. Various bead sizes (0.21 and 0.81 micron) were tested using particles which had been covalently modified (or coated) with biotinylated rabbit anti-mouse immunoglobulin antibodies. Detection limits of mouse monoclonal antibodies or antigens (which have been reacted with specific mouse antibodies) were compared to those obtained using the general homogeneous 'sandwich' ELISA assay. Compared to the homogeneous biotin-avidin ELISA an increase in sensitivity level of about 6-10 times was observed in antigen detection assays. The stability of the reagent was excellent. The method gave low backgrounds and was as simple to use as the standard procedure.
Nilsson
B
B
Research and Development, Carbohydrates International AB, Arlöv, Sweden.
D000469:Alkaline Phosphatase; D000818:Animals; D001360:Avidin; D001710:Biotin; D004355:Drug Stability; D004797:Enzyme-Linked Immunosorbent Assay; D007202:Indicators and Reagents; D007840:Latex; D051379:Mice; D008863:Microspheres; D010316:Particle Size; D011817:Rabbits
D007202:Indicators and Reagents; D007840:Latex; D001360:Avidin; D001710:Biotin; D000469:Alkaline Phosphatase