title: Laboratory diagnosis of peritonitis in patients on continuous ambulatory peritoneal dialysis.
abstract: The clinical course and laboratory diagnosis of peritonitis in patients undergoing continuous ambulatory peritoneal dialysis was studied in 32 consecutive episodes. Peritonitis was associated with a failure in aseptic technique in eight episodes and with an exit-site infection in four episodes. Intraperitoneal vancomycin and ceftazidime were safe, effective, and convenient. Most patients administered their antibiotics at home, and symptoms usually resolved by day 4. Culture of the deposit obtained by centrifugation of 50 ml of effluent after leukocyte lysis provided the best rate of recovery (84% culture positive) but was technically demanding. Filtration of the same volume without leukocyte lysis was simple to perform and almost as effective. Enrichment was less satisfactory (65% culture positive) owing to the presence of antibiotic or infection with fastidious microorganisms. Culture of 50 ml of effluent after concentration by a commonly used laboratory technique, centrifugation without leukocyte lysis, performed poorly (59% culture positive at 48 h), as this method caused sequestration and death of microorganisms within the leukocytes. Culture of nearly 1 liter of effluent from 33 asymptomatic patients by the same techniques yielded no microorganisms.
authors.affiliation: Department of Microbiology, St. Thomas' Hospital, London, England.
authors.forename: H A
authors.initials: HA
authors.lastname: Ludlam
authors.forename: T N
authors.initials: TN
authors.lastname: Price
authors.forename: A J
authors.initials: AJ
authors.lastname: Berry
authors.forename: I
authors.initials: I
authors.lastname: Phillips
mesh_terms: D001419:Bacteria / Q000254:growth & development / Q000302:isolation & purification; D001424:Bacterial Infections / Q000175:diagnosis*; D015169:Colony Count, Microbial; D005840:Gentian Violet; D006801:Humans; D007958:Leukocyte Count; D010531:Peritoneal Dialysis, Continuous Ambulatory; D010538:Peritonitis / Q000175:diagnosis*; D010619:Phenazines
chemical_list: C035863:Gram's stain; D010619:Phenazines; D005840:Gentian Violet