Functional responses of aequorin-loaded human neutrophils. Comparison with fura-2-loaded cells.
Aequorin-loaded human neutrophils in response to chemotactic peptides and ionomycin showed a sharp rise in their intracellular Ca2+ concentration which decayed within 2 min. Depletion of extracellular Ca2+ suppressed only the ionomycin-induced increase. Fura-2-loaded cells also showed a sharp rise in the intracellular Ca2+ concentration in response to each stimulator, while the decline was extremely slow in the ionomycin-induced Ca2+ increase. Depletion of extracellular Ca2+ reduced the duration of ionomycin-induced Ca2+ increase. Cytochalasin B almost equally potentiated the rise in the intracellular Ca2+ concentration induced by each stimulator. Aequorin-loaded cells showed impaired phagocytotic activity, while degranulation and oxygen radical production were not affected.
Ozaki
Y
Y
Central Clinical Laboratory, Yamanashi Medical College, Nakakoma-gun, Japan.
Kume
S
S
D000331:Aequorin / Q000494:pharmacology*; D001572:Benzofurans / Q000494:pharmacology*; D002118:Calcium / Q000097:blood*; D003571:Cytochalasin B / Q000494:pharmacology; D004987:Ethers / Q000494:pharmacology; D016257:Fura-2; D006801:Humans; D015759:Ionomycin; D007700:Kinetics; D008164:Luminescent Proteins / Q000494:pharmacology*; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*
D001572:Benzofurans; D004987:Ethers; D008164:Luminescent Proteins; D003571:Cytochalasin B; D000331:Aequorin; D015759:Ionomycin; D009240:N-Formylmethionine Leucyl-Phenylalanine; D002118:Calcium; D016257:Fura-2