title: Evidence of in vitro germinal layer development in Echinococcus granulosus cysts.
abstract: In vitro protoscolices of Echinococcus granulosus grew in a vesicular direction. After 9 days the laminated layer was observed; it reached thicknesses up to 140 micron. By day 125 of culture, the cysts had reached sizes of up to 4.875 micron and a high percentage of cysts had been obtained (10.4%). The ultrastructure of these cysts was studied and the existence of a germinal layer was demonstrated.
authors.affiliation: Laboratory of Parasitology, Faculty of Pharmacy, University of Alcalá de Henares, Spain.
authors.forename: F
authors.initials: F
authors.lastname: Rodriguez-Caabeiro
authors.forename: N
authors.initials: N
authors.lastname: Casado
mesh_terms: D000818:Animals; D004446:Echinococcus / Q000254:growth & development* / Q000648:ultrastructure; D008854:Microscopy, Electron