Choline accumulation in isolated rat hepatocytes.
The accumulation of non-metabolized choline in isolated rat hepatocytes is concentrative in Na+ medium, whereas the accumulation does not exceed unity in a Li+ medium. Ouabain and 2,4-dinitrophenol inhibited the choline uptake. These results indicate that choline is taken up by rat hepatocytes via a Na+- and energy-dependent process, and choline oxidase is not directly connected with the choline transport system.
Yoshioka
K
K
Department of Medical Biochemistry, Shiga University of Medical Science, Ohtsu, Japan.
Nishimura
H
H
D019297:2,4-Dinitrophenol; D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D002794:Choline / Q000378:metabolism*; D004140:Dinitrophenols / Q000494:pharmacology; D007700:Kinetics; D008094:Lithium / Q000494:pharmacology; D008099:Liver / Q000378:metabolism*; D008297:Male; D010042:Ouabain / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D012964:Sodium / Q000494:pharmacology
D004140:Dinitrophenols; D010042:Ouabain; D008094:Lithium; D012964:Sodium; D002794:Choline; D019297:2,4-Dinitrophenol