title: Porcine epidemic diarrhea virus (CV 777) and feline infectious peritonitis virus (FIPV) are antigenically related.
abstract: Using gut sections from pigs infected with porcine epidemic diarrhea virus (strain CV 777) and ascitic fluid from cats which had succumbed to feline infectious peritonitis (FIP), a weak cross reaction was found by immunofluorescence. Its specificity was confirmed when detergent-treated purified CV 777 showed a prominent reaction with FIPV antibodies in ELISA; no reaction was obtained with intact virions, which indicated common determinants on an internal component of the particle. Antigenic cross-reactions at the nucleocapsid level were found in Western blot ELISA performed both ways (CV 777/FIPV antibodies; FIPV/CV 777 antibodies). In immunoprecipitation using [35S]methionine labelled FIPV, anti-CV 777 sera recognized exclusively the nucleocapsid protein. The significance of these findings for the classification of coronaviruses is discussed.
authors.affiliation: Institute of Virology, Veterinary Faculty, State University, Utrecht, The Netherlands.
authors.forename: Y L
authors.initials: YL
authors.lastname: Zhou
authors.forename: J
authors.initials: J
authors.lastname: Ederveen
authors.forename: H
authors.initials: H
authors.lastname: Egberink
authors.forename: M
authors.initials: M
authors.lastname: Pensaert
authors.forename: M C
authors.initials: MC
authors.lastname: Horzinek
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000276:immunology*; D015153:Blotting, Western; D002213:Capsid / Q000276:immunology*; D002415:Cats / Q000382:microbiology; D003332:Coronaviridae / Q000276:immunology*; D003429:Cross Reactions; D003967:Diarrhea / Q000382:microbiology; D010538:Peritonitis / Q000382:microbiology; D011233:Precipitin Tests; D013552:Swine / Q000382:microbiology; D014758:Viral Core Proteins / Q000276:immunology*; D014759:Viral Envelope Proteins / Q000276:immunology
chemical_list: D000956:Antigens, Viral; D014758:Viral Core Proteins; D014759:Viral Envelope Proteins