Wheat germ agglutinin fracture-label of Golgi apparatus membranes in proliferating cells.
The thin section fracture-label technique has been recently used to analyze the distribution and compartmentalization of fully glycosylated components on intracellular membranes. Labelling with the lectin wheat germ agglutinin over the freeze-fractured membranes of Golgi apparatus in various secretory and non-secretory cells as well as in human peripheral lymphocytes was always very weak or absent even over the trans-most cisternae. In order to investigate if the labelling density may reflect the cellular activity in membrane biogenesis, we used in this study wheat germ agglutinin fracture-label of rapidly proliferating cells and mitogen-activated lymphocytes. Labelling over the fractured cisternae of the medial and trans portions of the Golgi apparatus was intense. Treatment with cycloheximide of proliferating cells induced a drastic reduction of the labelling over the Golgi cisternae.
Lotti
L V
LV
Dipartimento di Medicina Sperimentale, Università degli Studi di Roma La Sapienza, Italy.
Pavan
A
A
Mancini
P
P
Frati
L
L
Torrisi
M R
MR
D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D003513:Cycloheximide / Q000494:pharmacology; D005614:Freeze Fracturing / Q000379:methods*; D006023:Glycoproteins / Q000032:analysis*; D006031:Glycosylation; D006056:Golgi Apparatus / Q000032:analysis* / Q000187:drug effects / Q000648:ultrastructure; D006801:Humans; D008214:Lymphocytes / Q000032:analysis* / Q000187:drug effects / Q000648:ultrastructure; D008854:Microscopy, Electron; D014909:Wheat Germ Agglutinins
D006023:Glycoproteins; D014909:Wheat Germ Agglutinins; D003513:Cycloheximide