Measurement of specific radioactivity of tryptophan labeled with carbon-14 in plasma by high-performance liquid chromatography with a synchronized accumulating radioisotope detector.
Measurement of specific radioactivity by a high-performance liquid chromatograph with a synchronized accumulating radioisotope detector was conducted. Accuracy of measurement for an authentic sample containing 0.2 nCi of tryptophan labeled with carbon-14 exceeded 95%. In the case of a plasma sample obtained 120 min following intravenous administration of 15 muCi of labeled tryptophan to a rat, the coefficient of variation was 7.0%.
Baba
S
S
Laboratory of Physical and Pharmaceutical Chemistry, Tokyo College of Pharmacy, Japan.
Yamaguchi
J
J
Horie
M
M
D000818:Animals; D002250:Carbon Radioisotopes; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D051381:Rats; D014364:Tryptophan / Q000097:blood*
D002250:Carbon Radioisotopes; D014364:Tryptophan