Preservation of hepatocyte suspensions at 4 degrees C.
Suspensions of rat hepatocytes are resistant to hypoxia for at least 24 hr at 4 degrees C. After storage for 24 hr with xylitol, sorbitol, or glycerol, their subsequent capacity for glucogenesis from these substrates is increased. Even storage under N2/CO2 as the gas phase has little deleterious effect.
Lund
P
P
Nuffield Department of Clinical Medicine, Radcliffe Infirmary, Oxford, United Kingdom.
Wiggins
D
D
D000818:Animals; D003080:Cold Temperature; D005947:Glucose / Q000096:biosynthesis; D008099:Liver / Q000166:cytology*; D051381:Rats; D014021:Tissue Preservation
D005947:Glucose