Determination of nitrilotriacetate in biological matrices using ion exclusion chromatography.
A sensitive method for the determination of nitrilotriacetate in biological growth media and cell-free extracts by ion exclusion chromatography is described using HCl as an eluant. The eluant conductivity was chemically suppressed with a membrane suppressor and a conductivity detector was used for subsequent detection. The membrane was continuously regenerated with a tetrabutylammoniumhydroxide solution. The detection limit for nitrilotriacetate in cell-free extract was 11 mg/liter, while for nitrilotriacetate in growth media it was 1 mg/liter. Interference by compounds present in biological matrices with the determination is discussed.
Schneider
R P
RP
Institute of Aquatic Sciences, Swiss Federal Institute of Technology, Zürich.
Zürcher
F
F
Egli
T
T
Hamer
G
G
D000085:Acetates / Q000032:analysis*; D002138:Calibration; D002474:Cell-Free System; D002845:Chromatography / Q000295:instrumentation; D003470:Culture Media; D009571:Nitrilotriacetic Acid / Q000032:analysis*; D011549:Pseudomonas
D000085:Acetates; D003470:Culture Media; D009571:Nitrilotriacetic Acid