Iron binding proteins and their roles in the tobacco hornworm, Manduca sexta (L.).
Manduca sexta larvae accumulate large amounts of iron during their larval feeding period. When 59Fe was fed to 5th instar larvae, it was evenly distributed among the hemolymph, gut and carcass until the cessation of feeding. By pupation 95% of the labelled iron was found in the fat body. In the adult a significant portion of this iron was found in flight muscle. Studies of the hemolymph disclosed two iron-containing proteins. The first was composed of a single polypeptide chain of 80 kD, containing one atom of iron. This protein bound ionic iron in vitro and was able to transfer this iron to ferritin when incubated with fat body in vitro. Therefore, it appeared to serve a transport function. The second protein had a molecular weight of 490 kD with subunits of 24 and 26 kD and contained 220 micrograms of iron/mg protein. Its chemical and ultrastructural characteristics were those of ferritin. These studies demonstrate the presence of both a transport protein and a unique circulating ferritin in Manduca sexta, the latter serving a storage function during development and possibly also a transport function.
Huebers
H A
HA
Department of Medicine, University of Washington, Seattle 98195.
Huebers
E
E
Finch
C A
CA
Webb
B A
BA
Truman
J W
JW
Riddiford
L M
LM
Martin
A W
AW
Massover
W H
WH
D000818:Animals; D002352:Carrier Proteins / Q000502:physiology*; D002852:Chromatography, Ion Exchange; D005216:Fat Body / Q000032:analysis; D005293:Ferritins / Q000032:analysis; D006458:Hemolymph / Q000032:analysis; D007501:Iron / Q000032:analysis / Q000378:metabolism*; D033862:Iron-Binding Proteins; D007814:Larva; D007915:Lepidoptera / Q000502:physiology*; D009036:Moths / Q000254:growth & development / Q000502:physiology*; D033863:Transferrin-Binding Proteins
D002352:Carrier Proteins; D033862:Iron-Binding Proteins; D033863:Transferrin-Binding Proteins; D005293:Ferritins; D007501:Iron