title: Immunoblotting studies of the molecular forms of protein C in plasma.
abstract: Anti-plasma protein C monoclonal antibodies were prepared and characterized, and quantitative immunoblotting techniques were developed to determine the molecular forms of protein C in whole plasma. Two antibodies reacted with the heavy chain of protein C, four reacted with the light chain, and two reacted only with nonreduced protein C. A doublet of protein C (MW = 63-66K) was seen on nonreduced immunoblots of normal plasma and 30 heterozygous protein C deficient plasmas (2-77% protein C antigen). In reduced plasma, approximately 75% of protein C presented as doublet heavy chains (MW = 39-42K) and doublet light chains (MW = 22-25K), and approximately 25% was single chain (MW = 64K). The immunoblotting technique was quantitative, specific, sensitive, and correlated with electroimmunoassay results. It also provided visual qualitative information not obtainable with other methods of quantitation.
authors.affiliation: Department of Immunology, Research Institute of Scripps Clinic, La Jolla, CA 92037.
authors.forename: M J
authors.initials: MJ
authors.lastname: Heeb
authors.forename: H P
authors.initials: HP
authors.lastname: Schwarz
authors.forename: T
authors.initials: T
authors.lastname: White
authors.forename: B
authors.initials: B
authors.lastname: Lämmle
authors.forename: M
authors.initials: M
authors.lastname: Berrettini
authors.forename: J H
authors.initials: JH
authors.lastname: Griffin
mesh_terms: D000911:Antibodies, Monoclonal; D006801:Humans; D007118:Immunoassay; D015151:Immunoblotting / Q000379:methods*; D008970:Molecular Weight; D011486:Protein C / Q000276:immunology / Q000302:isolation & purification*; D020151:Protein C Deficiency; D011487:Protein Conformation
chemical_list: D000911:Antibodies, Monoclonal; D011486:Protein C