title: 5 alpha-cholest-8(14)-en-3 beta-ol-15-one, a potent regulator of cholesterol metabolism: occurrence in rat skin.
abstract: 5 alpha-Cholest-8(14)-en-3 beta-ol-15-one is a potent inhibitor of cholesterol biosynthesis which has significant hypocholesterolemic activity upon oral administration to animals. Described herein are the results of experiments that indicate the presence of the 15-ketosterol in rat skin. The 15-ketosterol was, after purification by medium pressure liquid chromatography on Lichroprep RP-8 columns, thin-layer chromatography on silica gel G, and reverse phase high performance liquid chromatography, characterized by gas-liquid chromatography-mass spectrometry in the form of its trimethylsilyl ether derivative. The use of an internal standard containing both tritium and deuterium permitted the determination of the levels of the 15-ketosterol by mass fragmentography. The results of five separate analyses of portions of the skin of a male Sprague Dawley rat showed a mean value of 84.5 +/- 4.1 (SEM) ng per g. Analyses of hair samples of ten male Sprague Dawley rats indicated a mean level of 143 +/- 19 (SEM) ng per g of hair. Most (approximately 72%) of the 15-ketosterol in hair was esterified. This report constitutes the first isolation of the 15-ketosterol from animal tissues.
authors.affiliation: Department of Biochemistry, Rice University, Houston, TX 77251.
authors.forename: G T
authors.initials: GT
authors.lastname: Emmons
authors.forename: J
authors.initials: J
authors.lastname: St Pyrek
authors.forename: R
authors.initials: R
authors.lastname: Dam
authors.forename: M
authors.initials: M
authors.lastname: Martin
authors.forename: K
authors.initials: K
authors.lastname: Kudo
authors.forename: G J
authors.initials: GJ
authors.lastname: Schroepfer
mesh_terms: D000818:Animals; D000924:Anticholesteremic Agents / Q000032:analysis*; D002782:Cholestenes / Q000032:analysis*; D002783:Cholestenones / Q000032:analysis*; D002784:Cholesterol / Q000378:metabolism*; D004952:Esters / Q000032:analysis; D006197:Hair / Q000032:analysis; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012867:Skin / Q000032:analysis*
chemical_list: D000924:Anticholesteremic Agents; D002782:Cholestenes; D002783:Cholestenones; D004952:Esters; C015073:cholest-8(14)-en-3-ol-15-one; D002784:Cholesterol