Inhibitors of sterol synthesis. 14 alpha-ethyl-5 alpha-cholest-7-ene-3 beta,15 alpha-diol induces changes in the sterol composition and the morphology of CHO-K1 cells.
Incubation of CHO-K1 cells in lipid-deficient medium containing 14 alpha-ethyl-5 alpha-cholest-7-ene-3 beta,15 alpha-diol (0.1 microM) for 4 days was associated with a profound change in cellular sterol composition as reflected by a marked accumulation of lanosterol and 24,25-dihydrolanosterol. A striking elongation of the cells was also observed. Incubation of CHO-K1 cells in lipid-deficient medium containing lanosterol (10 microM) also caused a significant accumulation of lanosterol which was also associated with a marked elongation of the cells.
Pinkerton
F D
FD
Department of Biochemistry, Rice University, Houston, Texas 77251.
Izumi
A
A
Schroepfer
G J
GJ
D000818:Animals; D002478:Cells, Cultured / Q000166:cytology / Q000187:drug effects*; D002782:Cholestenes / Q000494:pharmacology*; D002784:Cholesterol / Q000494:pharmacology; D006224:Cricetinae; D007810:Lanosterol / Q000378:metabolism / Q000494:pharmacology; D013261:Sterols / Q000378:metabolism*
D002782:Cholestenes; D013261:Sterols; D007810:Lanosterol; C015200:14-ethylcholest-7-ene-3,15-diol; D002784:Cholesterol