title: Proteoglycan biosynthesis by rabbit articular chondrocytes treated with D-penicillamine.
abstract: Rabbit articular chondrocytes in confluent monolayer cultures were treated with D-Penicillamine (D-Pen) during 3 or 5 days. The [35S]-sulfate incorporation in neosynthesized proteoglycans was not modified by D-Pen doses ranging from 50 to 800 micrograms/ml. After treatment during 5 days with D-Pen concentrations of 50 or 400 micrograms/ml, the chemical characteristics of proteoglycans from medium and cell-layer were determined. The aggregation capacity of proteoglycans from medium, the monomer molecular size, the glycosaminoglycan chain length and the relative rates of the different glycosaminoglycans (chondroitins, chondroitin 6-sulfate, chondroitin 4-sulfate, hyaluronic acid) remained unchanged. These results suggest that D-Pen does not alter some of the cartilage mechanical properties due to the presence of proteoglycans.
authors.affiliation: Laboratoire de Biochimie du Tissu Conjonctif, U.F.R. de Sciences, Caen, France.
authors.forename: P
authors.initials: P
authors.lastname: Legendre
authors.forename: M
authors.initials: M
authors.lastname: Bouakka
authors.forename: M
authors.initials: M
authors.lastname: Langris
authors.forename: J P
authors.initials: JP
authors.lastname: Pujol
authors.forename: R
authors.initials: R
authors.lastname: Beliard
authors.forename: G
authors.initials: G
authors.lastname: Loyau
authors.forename: J
authors.initials: J
authors.lastname: Bocquet
mesh_terms: D000818:Animals; D002358:Cartilage, Articular / Q000187:drug effects* / Q000378:metabolism; D002478:Cells, Cultured; D002850:Chromatography, Gel; D005109:Extracellular Matrix / Q000378:metabolism; D006025:Glycosaminoglycans / Q000096:biosynthesis / Q000302:isolation & purification; D008968:Molecular Conformation; D008970:Molecular Weight; D010396:Penicillamine / Q000494:pharmacology*; D011509:Proteoglycans / Q000096:biosynthesis* / Q000302:isolation & purification; D011817:Rabbits
chemical_list: D006025:Glycosaminoglycans; D011509:Proteoglycans; D010396:Penicillamine