Functional heterogeneity of rat hepatocytes: predominance of aryl hydrocarbon hydroxylase activity in perivenular zone.
To elucidate the hepatic intralobular distribution of aryl hydrocarbon hydroxylase (AHH) activity biochemically, periportal (PP) and perivenular hepatocytes (PV) from male Sprague-Dawley rats were separated by a fluorescence-activated cell sorter after labeling the PP zone with fluorescein diacetate and the perivenular zone with fluorescein isothiocyanate. AHH activity was higher in PV than in PP. The enzyme activity was induced about 6-fold in hepatocytes of rats pretreated with 3-methyl-cholanthrene, and the induction was more prominent in PP than in PV. Neither phenobarbital pretreatment nor altered lipid content of the diet induced the change in the enzyme activity.
Tazawa
J
J
Department of Pharmacology, Faculty of Medicine, University of Tokyo, Japan.
Endou
H
H
Sato
A
A
Hasumura
Y
Y
Takeuchi
J
J
D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases / Q000378:metabolism*; D002469:Cell Separation; D004790:Enzyme Induction; D008099:Liver / Q000201:enzymology*; D008297:Male; D008748:Methylcholanthrene / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains
D008748:Methylcholanthrene; D001189:Aryl Hydrocarbon Hydroxylases