Calcium transport and energy coupling in diabetic rat liver mitochondria.
Liver mitochondria from chronic diabetic rats took up Ca ions at a significantly slower rate than their normal counterparts. The same mitochondria, inhibited with ruthenium red, released Ca ions at a faster rate than normal mitochondria. In good agreement with the decrease of Ca ions uptake, measurement of the inner membrane potential by the safranine method yielded a significantly lower value with diabetic than with normal mitochondria. Respiratory control and P/O ratios were also decreased in diabetic mitochondria.
Grinblat
L
L
Centro de Investigaciones Bioenergéticas, Facultad de Medicina, Universidad de Buenos Aires, Paraguay, Argentina.
Bedetti
C D
CD
Stoppani
A O
AO
D000818:Animals; D001693:Biological Transport, Active; D002118:Calcium / Q000378:metabolism*; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D004734:Energy Metabolism; D066298:In Vitro Techniques; D008297:Male; D008564:Membrane Potentials; D008930:Mitochondria, Liver / Q000378:metabolism*; D010101:Oxygen Consumption; D051381:Rats
D002118:Calcium