Activation and stabilization of diaphragm-associated acetylcholinesterase by monovalent (Na+, Li+) cations.
Acetylcholinesterase (AChE) activity was determined at varied pH values between 6 and 11 in rat homogenated diaphragm and in eel E. electricus soluble AChE, in the presence or absence of 115 mM NaCl or LiCl. It was observed that by using homogenated diaphragm Li+ stimulated AChE at physiological pH (7-7.4). In control (no cations) a pH "optimum" of 8.6-9 was found, while in presence of NaCl or LiCl "optima" of 9.5 and 10.2 were observed respectively. At optimum pH, AChE activity was about 2 times higher with NaCl, while with LiCl 5 times higher than the control. Preincubation of the enzyme or the homogenate in cations presence at pH 5.5 or pH 12.8 had no effect on the activity, when it was measured at pH "optima". However, without cations only 76% of the activity in optimum pH after preincubation at pH 5.5 was found. These results suggest that: (a) Li+ may neutralize negative charges of AChE more successfully than Na+, resulting in better enzyme activation and stabilization; (b) a possible enzyme desensitization induced by pH changes can be avoided by increasing Na+ concentrations and especially Li+.
Tsakiris
S
S
Department of Experimental Physiology, University of Athens, Medical School, Greece.
Kouniniotou-Krontiri
P
P
D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D003964:Diaphragm / Q000201:enzymology; D004593:Electrophorus; D004789:Enzyme Activation; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008094:Lithium / Q000494:pharmacology*; D051381:Rats; D012964:Sodium / Q000494:pharmacology*
D008094:Lithium; D012964:Sodium; D000110:Acetylcholinesterase