Removal of dodecyl sulfate from protein solution.
Sodium dodecyl sulfate is one of the most widely used detergents. But, there is no good method to remove dodecyl sulfate (DS) from a small volume of protein solution. We showed that (a) DS in protein solution was removed by the addition of potassium salts with high recovery of protein, and (b) the method was useful before tryptic digestion of protein.
Suzuki
H
H
Department of Biophysical Chemistry, Kitasato University School of Medicine, Kanagawa, Japan.
Terada
T
T
D001464:Barium; D017609:Barium Compounds; D002122:Calcium Chloride; D011232:Chemical Precipitation; D002712:Chlorides; D006868:Hydrolysis; D011188:Potassium; D011506:Proteins; D012492:Salts; D012710:Serum Albumin, Bovine; D012967:Sodium Dodecyl Sulfate / Q000302:isolation & purification*; D012996:Solutions; D014357:Trypsin
D017609:Barium Compounds; D002712:Chlorides; D011506:Proteins; D012492:Salts; D012996:Solutions; C024986:barium chloride; D001464:Barium; D012710:Serum Albumin, Bovine; D012967:Sodium Dodecyl Sulfate; C028913:dodecyl sulfate; D014357:Trypsin; D002122:Calcium Chloride; D011188:Potassium