Effect of general anesthesia on syncytiotrophoblast plasma membranes from human placenta.
This investigation shows that a general anesthetic produces similar effects in vivo and in vitro. Anesthesia with a barbituric drug, Thiopental, induces an increase in membrane fluidity and a decrease in the activity of acetylcholinesterase in syncytiotrophoblast plasma membranes (SPM) obtained from placentas after caesarean section. The same effects can be reproduced in vitro after anesthetic addition to the isolated plasma membranes. Morphological and freeze-fracturing studies also suggest that membrane protein components are affected by anesthetics.
Mazzanti
L
L
Istituto di Biochimica, Facoltà di Medicina e Chirurgia, Ancona.
Cester
N
N
Memè
E
E
Valensise
H
H
Romanini
C
C
Ferrara
P
P
Marinelli
F
F
Biagini
G
G
Bertoli
E
E
Lenaz
G
G
D000110:Acetylcholinesterase / Q000378:metabolism; D002462:Cell Membrane / Q000187:drug effects / Q000201:enzymology / Q000648:ultrastructure; D005260:Female; D005614:Freeze Fracturing; D006801:Humans; D066298:In Vitro Techniques; D008560:Membrane Fluidity / Q000187:drug effects; D008565:Membrane Proteins / Q000378:metabolism; D010920:Placenta / Q000187:drug effects* / Q000201:enzymology / Q000648:ultrastructure; D011247:Pregnancy; D013874:Thiopental / Q000494:pharmacology*
D008565:Membrane Proteins; D000110:Acetylcholinesterase; D013874:Thiopental