A convenient bioassay for NGF using a new subline of PC12 pheochromocytoma cells (PC12D).
A useful bioassay for nerve growth factor (NGF) has been developed, based on the rapid outgrowth of neurites (within 24 h) from cells of a new subclone of PC12 cells (PC12D) in response to NGF. The sensitivity is similar to that of other bioassay systems that the the sensory ganglia of chick embryos of primed PC12 cells. The assay is readily adaptable for the purification of NGF and for the determination of levels of NGF in tissue, as shown by a comparison of results from this assay to the data obtained by an immunological assay.
Sano
M
M
Department of Morphology, Institute for Developmental Research, Aichi Prefectural Colony, Japan.
Kato
K
K
Totsuka
T
T
Katoh-Semba
R
R
D000818:Animals; D001681:Biological Assay / Q000379:methods*; D003712:Dendrites / Q000187:drug effects; D005260:Female; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009414:Nerve Growth Factors / Q000494:pharmacology*; D010673:Pheochromocytoma; D051381:Rats; D014407:Tumor Cells, Cultured / Q000187:drug effects*
D009414:Nerve Growth Factors