[Complementary addressed modification of single- and double-stranded DNA by alkylating derivatives of oligonucleotides isolated by partial DNA fragmentation].
Reagents for complementary addressed modification of nucleic acids are proposed to be synthesized on the base of oligonucleotides obtained by partial chemical fragmentation of DNA. The alkylating 4-(N-2 chlorethyl-N-methylamino) benzyl-5'-phosphamide derivatives of 5'-[32P]-labelled oligonucleotides obtained from single and double-stranded DNA cloned in bacteriophage M13 mp9 have been synthesized. The alkylated derivatives of oligonucleotides selectively modify the complementary tracts of single-stranded DNA-target. They are also able to modify the complementary regions in double-stranded supercoiled plasmid DNA.
Gaĭdamakov
S A
SA
Tsirel'nikov
N I
NI
Vlasov
V V
VV
D000477:Alkylating Agents / Q000494:pharmacology*; D003090:Coliphages; D004247:DNA / Q000187:drug effects* / Q000378:metabolism; D004277:DNA, Single-Stranded / Q000187:drug effects* / Q000378:metabolism; D004279:DNA, Viral / Q000187:drug effects / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D007202:Indicators and Reagents; D009691:Nucleic Acid Denaturation; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000378:metabolism / Q000494:pharmacology*
D000477:Alkylating Agents; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D007202:Indicators and Reagents; D009841:Oligonucleotides; D004247:DNA
Komplementarno adressovannaia modifikatsiia odnotsepochechnykh i dvuspiral'nykh DNK alkiliruiushchimi proizvodnymi oligonukleotidov, poluchennykh ogranichennoĭ fragmentatsieĭ DNK.