Osteogenesis in in vivo diffusion chamber cultures of human marrow cells.
The osteogenic diffusion chamber culture of rodent marrow cells is a well established system. In the present study, marrow cells from children and adult human donors were incubated in diffusion chambers implanted intraperitoneally in athymic mice. After 4 or 8 weeks, the chamber content was examined by light and electron microscopy. Child-cell cultures showed osteogenic tissue consisting of a mineralizing fibrous component and cartilage. Ultrastructurally, the fibrous tissue was similar to osteoid and exhibited osteoblast-like cells and mineralizing nodules. Mineral aggregates were also found in the cartilage. These features in child-cell chambers were similar to those found in control chambers of rabbit marrow cells. Adult-cell chambers showed only unmineralized fibrous tissue. These results render previous findings in animal-cell diffusion chamber systems relevant to the understanding of bone formation in man. It is suggested that the difference between child- and adult-cell chambers reflects an age-related decline in the number of marrow osteoprogenitor cells or their potential to undergo terminal osteogenic differentiation.
Bab
I
I
Division of Oral Pathology, Hebrew University-Hadassah Faculty of Dental Medicine, Jerusalem, Israel.
Passi-Even
L
L
Gazit
D
D
Sekeles
E
E
Ashton
B A
BA
Peylan-Ramu
N
N
Ziv
I
I
Ulmansky
M
M
D000328:Adult; D000818:Animals; D001854:Bone Marrow Cells; D002113:Calcification, Physiologic; D002356:Cartilage / Q000166:cytology / Q000502:physiology; D002478:Cells, Cultured; D002648:Child; D015194:Diffusion Chambers, Culture; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008854:Microscopy, Electron; D008903:Minerals / Q000378:metabolism; D010012:Osteogenesis; D010529:Peritoneal Cavity; D011817:Rabbits
D008903:Minerals