title: Cytotoxic effects of ingested Staphylococcus aureus on bovine endothelial cells: role of S. aureus alpha-hemolysin.
abstract: Previously we observed that Staphylococcus aureus phagocytized by cultured bovine endothelial cells do not proliferate intracellularly, but are cytotoxic to bovine endothelial cells. To investigate S. aureus virulence factors which may be produced intracellularly and cause lysis of endothelial cells, we tested S. aureus mutants defective in production of one or more potential virulence factors and corresponding parent strains for cytotoxicity to endothelial cell monolayers subsequent to being ingested. Following incubation of endothelial cell monolayers with S. aureus for 3.5 h, cultures were supplemented with lysostaphin to destroy extracellular but not intracellular S. aureus. At subsequent times, viability of endothelial cells was assayed by retention of 3H-adenine and the number of intracellular S. aureus was measured. The cytotoxic activity of S. aureus culture supernatants was also characterized. The results indicate that S. aureus alpha-hemolysin is cytotoxic to bovine endothelial cells and plays an important role in the damage suffered by bovine endothelial cell monolayers following ingestion of S. aureus. Ingestion of alpha-hemolysin-producing S. aureus by endothelial cells in vivo might be expected to result in destruction of endothelium followed by development of platelet-fibrin vegetations. This possible sequence of events is compatible with the frequently fulminant course of S. aureus endocarditis.
authors.affiliation: Department of Medical Microbiology, University of Wisconsin Medical School, Madison 53706.
authors.forename: J M
authors.initials: JM
authors.lastname: Vann
authors.forename: R A
authors.initials: RA
authors.lastname: Proctor
mesh_terms: D000818:Animals; D001427:Bacterial Toxins / Q000633:toxicity*; D002417:Cattle; D002470:Cell Survival; D002478:Cells, Cultured; D003470:Culture Media / Q000633:toxicity; D004730:Endothelium, Vascular / Q000166:cytology*; D006460:Hemolysin Proteins / Q000633:toxicity*; D009154:Mutation; D010587:Phagocytosis; D013211:Staphylococcus aureus / Q000235:genetics / Q000472:pathogenicity*; D014774:Virulence
chemical_list: D001427:Bacterial Toxins; D003470:Culture Media; D006460:Hemolysin Proteins; C021091:staphylococcal alpha-toxin