Climbing fibers are labelled after injection of PHA-L into the nucleus interpositus of the cat.
After injection of Phaseolus vulgaris-leucoagglutinin (PHA-L) into the interposed nucleus of the cat, labelled fibers were found in the granular layer as well as in the molecular layer of the cerebellar cortex. Those in the molecular layer were judged from their location and morphology as climbing fibers. Such labelling of climbing fibers which have not been labelled by autoradiography could be attributed to higher sensitivity and resolution of PHA-L method. In addition, Purkinje cell soma and its dendrites were labelled in a narrow stripe in the vermal and paravermal cortex. The pattern of labelling suggests that they were labelled by retrograde transport of the lectin.
Murakami
F
F
Department of Biophysical Engineering, Faculty of Engineering Science, Osaka University, Japan.
Song
W J
WJ
D000818:Animals; D002415:Cats; D002525:Cerebellar Cortex / Q000166:cytology*; D002529:Cerebellar Nuclei / Q000166:cytology*; D009434:Neural Pathways / Q000033:anatomy & histology; D010835:Phytohemagglutinins
D010835:Phytohemagglutinins