Serum amphotericin-B assay by scanning spectrophotometer.
We describe a simple, specific, 3-min assay for amphotericin-B in serum based on the absorbance at 408 nm and spectral scanning between 450 and 350 nm of the drug in acetonitrile extracts. The method correlated well with a high-performance liquid chromatographic method.
Shihabi
Z K
ZK
Department of Pathology, Wake Forest University, Bowman Gray School of Medicine, Winston-Salem, North Carolina.
Wasilauskas
B L
BL
Peacock
J E
JE
D000666:Amphotericin B / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D013053:Spectrophotometry
D000666:Amphotericin B