New biotin-conjugated antisera for quantitation of Mycoplasma gallisepticum-specific immunoglobulin A in chicken.
The biotinylation of goat anti-alpha-chains of chicken immunoglobulin A (IgA), suitable for use in an avidin-biotin enzyme-linked immunosorbent assay, is described. The optimum conditions for the use of the developed conjugate in determining local and systemic IgA specific to Mycoplasma gallisepticum in chickens were established.
Barbour
E K
EK
Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Minnesota, St. Paul 55108.
Newman
J A
JA
Sivanandan
V
V
Sasipreeyajan
J
J
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis*; D000918:Antibody Specificity; D001360:Avidin; D001710:Biotin; D002645:Chickens / Q000276:immunology; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007070:Immunoglobulin A / Q000032:analysis*; D007071:Immunoglobulin A, Secretory / Q000032:analysis; D009174:Mycoplasma / Q000276:immunology*; D009175:Mycoplasma Infections / Q000276:immunology / Q000662:veterinary*; D011201:Poultry Diseases / Q000276:immunology*
D000907:Antibodies, Bacterial; D007070:Immunoglobulin A; D007071:Immunoglobulin A, Secretory; D001360:Avidin; D001710:Biotin