Transport of fluorescein in the rabbit eye after treatment with sodium iodate.
The outward active transport and the inward permeability of the blood-retinal barrier were studied in the rabbit eye after i.v. administration of sodium iodate. The active transport was evaluated from the half-time of disappearance of the vitreous fluorescein following intravitreal administration, and the inward permeability was evaluated from the vitreous concentration of fluorescein monoglucuronide after i.v. administration. The half-time of the vitreous fluorescein was 3.5 +/- 0.3 (mean +/- S.D.) hr, and 3.9 +/- 0.2 hr before and within 6 hr after iodate administration, respectively. After 24 hr, the half-time was 11.7 +/- 1.7 hr, similar to that of fluorescein monoglucuronide, 12.0 +/- 2.7 hr. The vitreous and the anterior chamber concentration of fluorescein monoglucuronide was measured at 1 hr after the i.v. dye injection. The vitreous concentration in the rabbits given iodate 3 hr before the dye injection was significantly greater than in the normal eyes, while the anterior chamber concentration was not different. Since fluorescein is rapidly metabolized to fluorescein monoglucuronide, differences in parameters determined using systemic fluorescein under two treatments or in disease states may be the result of alteration of the dynamics of fluorescein, fluorescein monoglucuronide, or both.
Kitano
S
S
Department of Ophthalmology, School of Medicine, University of Tokyo, Japan.
Hori
S
S
Nagataki
S
S
D000818:Animals; D000867:Anterior Chamber / Q000378:metabolism; D001693:Biological Transport, Active; D001769:Blood; D005123:Eye / Q000378:metabolism*; D019793:Fluorescein; D005452:Fluoresceins / Q000378:metabolism / Q000493:pharmacokinetics*; D007452:Iodates / Q000494:pharmacology*; D007455:Iodine / Q000494:pharmacology*; D008854:Microscopy, Electron; D010857:Pigment Epithelium of Eye / Q000648:ultrastructure; D011817:Rabbits; D013997:Time Factors; D014822:Vitreous Body / Q000378:metabolism
D005452:Fluoresceins; D007452:Iodates; C041357:fluorescein glucuronide; D007455:Iodine; D019793:Fluorescein; C032285:sodium iodate