title: Dependence with nutritional status of the ethanol effects on fatty acid metabolism in rat hepatocytes.
abstract: 1. The effects of ethanol on fatty acid synthesis, esterification and oxidation were studied in hepatocytes isolated from fed and 24 hr fasted rats. 2. [3H]H2O was preferentially incorporated into the glycerol backbone of triglycerides and phospholipids. Addition of ethanol markedly increased the incorporation of this label in both classes of glycerolipids; the increase was higher in fasted rat hepatocytes, both in the glycerol backbone and acyl groups of glycerolipids. 3. Ethanol increased [U-14C]palmitate incorporation into triglycerides only in hepatocytes from fasted rats. 4. [14C]CO2 and total acid soluble product formation from [1-14C]palmitate resulted inhibited by ethanol both in the fed and the fasted state.
authors.affiliation: Departamento de Bioquímica, Facultad de Química, Universidad Complutense, Madrid, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Maquedano
authors.forename: M
authors.initials: M
authors.lastname: Guzmán
authors.forename: J
authors.initials: J
authors.lastname: Castro
mesh_terms: D000818:Animals; D000431:Ethanol / Q000494:pharmacology*; D005227:Fatty Acids / Q000378:metabolism*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D009752:Nutritional Status; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D014280:Triglycerides / Q000378:metabolism
chemical_list: D005227:Fatty Acids; D010169:Palmitic Acids; D014280:Triglycerides; D019308:Palmitic Acid; D000431:Ethanol