Isolation and structural determination of rat neuromedin U.
Rat neuromedin U was isolated from the small intestine using mainly immunoaffinity chromatography and radioimmunoassay for pig neuromedin U-8. The amino acid sequence of rat neuromedin U was determined by microsequence analysis to be Tyr-Lys-Val-Asn-Glu-Tyr-Gln-Gly-Pro-Val-Ala-Pro-Ser-Gly-Gly- Phe-Phe-Leu-Phe-Arg-Pro-Arg-Asn-NH2, and this structure was confirmed by synthesis. Although the C-terminal heptapeptide amide structure of pig neuromedin U is completely conserved in rat neuromedin U, the remainder of the peptide reveals nine amino acid replacements and two amino acid deletions when compared to pig neuromedin U-25. Rat neuromedin U exerts two-fold potent uterus stimulant activity as compared to pig neuromedin U-25.
Minamino
N
N
Department of Biochemistry, Miyazaki Medical College, Japan.
Kangawa
K
K
Honzawa
M
M
Matsuo
H
H
D000595:Amino Acid Sequence; D000818:Animals; D002846:Chromatography, Affinity; D005260:Female; D007421:Intestine, Small / Q000032:analysis*; D008297:Male; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000138:chemical synthesis / Q000302:isolation & purification* / Q000502:physiology; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D013045:Species Specificity; D013552:Swine; D014590:Uterine Contraction
D009479:Neuropeptides; C046270:neuromedin U 8