Differential scanning calorimetric and Fourier transform infrared spectroscopic investigations of cerebroside polymorphism.
Calorimetric and Fourier transform infrared (FTIR) spectroscopic studies have been made of the polymorphism exhibited by bovine brain cerebroside-water systems, and the effect of cholesterol and dipalmitoylphosphatidylcholine (DPPC) upon this polymorphism was investigated. The conversion of the cerebroside from the thermodynamically stable to the metastable form is found to be accompanied by spectral changes, indicating a decrease in cerebroside headgroup hydration and a rearrangement of the hydrogen-bond network. The incorporation of low concentrations of cholesterol and DPPC into cerebroside bilayers broadens the thermal transitions associated with the cerebroside as a result of the disruption of cerebroside-cerebroside interactions. This disruption is evident in the spectra of cerebroside/cholesterol mixtures.
Jackson
M
M
Department of Protein & Molecular Biology, Royal Free Hospital School of Medicine, University of London, U.K.
Johnston
D S
DS
Chapman
D
D
D015060:1,2-Dipalmitoylphosphatidylcholine; D000818:Animals; D001923:Brain Chemistry; D002152:Calorimetry, Differential Scanning; D002417:Cattle; D002554:Cerebrosides; D005583:Fourier Analysis; D066298:In Vitro Techniques; D009186:Myelin Sheath / Q000502:physiology; D013055:Spectrophotometry, Infrared; D013816:Thermodynamics
D002554:Cerebrosides; D015060:1,2-Dipalmitoylphosphatidylcholine