[Initiation of lipid peroxidation as a result of hemoglobin transformation into hemichrome induced by free fatty acids].
The effects of free fatty acids on hemoglobin conversion and lipid peroxidation were studied in hemoglobin-containing liposomes (hemosomes) formed from an equimolar mixture of phosphatidylcholine (PC) and phosphatidylethanolamine (PE). It was shown that in hemosomes oxyhemoglobin is converted into hemichrome by the interaction of saturated fatty acids (arachidic, stearic, palmitic, myristic and lauric). This is accompanied by accumulation of primary and secondary products of lipid peroxidation. All fatty acids, except for lauric acid, have a stabilizing effect on lipid peroxidation in liposomes prepared from an equimolar mixture of PC and PE. The formation of lipid peroxidation products is inhibited by superoxide dismutase, D-alpha-tocopherol, D-mannitol and thiourea. The relationships between hemoglobin conversion and lipid peroxidation in hemosomes under effects of fatty acids were studied. The mechanisms of these reactions are discussed.
Andreiuk
G M
GM
Kiselev
P A
PA
D005230:Fatty Acids, Nonesterified / Q000494:pharmacology*; D005609:Free Radicals; D006420:Hemeproteins / Q000378:metabolism*; D006454:Hemoglobins / Q000378:metabolism*; D006461:Hemolysis; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D015227:Lipid Peroxidation; D008081:Liposomes; D010108:Oxyhemoglobins / Q000378:metabolism
D005230:Fatty Acids, Nonesterified; D005609:Free Radicals; D006420:Hemeproteins; D006454:Hemoglobins; D008081:Liposomes; D010108:Oxyhemoglobins; C001332:hemichrome
Initsiirovanie perekisnogo okisleniia lipidov v rezul'tate prevrashcheniia gemoglobina v gemikhrom pod deĭstviem svobodnykh zhirnykh kislot.