The chick embryo fibroblast cytosolic DNA complex--a possible cell-cell messenger.
1. The uptake of [3H]thymidine and [3H]uridine labelled DNA-RNA cytosol complex has been studied in chick embryo fibroblast cells. 2. The complex appears to be cleaved into DNA and RNA containing fragments in the recipient cell nucleus: both then enter the cell cytosol fraction, but the RNA fragment in particular is rapidly degraded. 3. Although [3H]thymidine labelled material present in the nucleus co-extracts with bulk nuclear DNA, caesium gradienting shows little or no evidence that integration of host and imported DNA has occurred. 4. It is suggested that the cytosolic/extruded DNA complex may be a "messenger" DNA, capable of the transfer of regulatory information between cells on a transient basis.
Adams
D H
DH
Department of Virology, Newcastle upon Tyne, England.
Challen
C
C
D000818:Animals; D002450:Cell Communication; D002642:Chick Embryo; D003600:Cytosol / Q000032:analysis; D004247:DNA / Q000032:analysis*; D005347:Fibroblasts / Q000032:analysis*; D015398:Signal Transduction; D013936:Thymidine / Q000378:metabolism; D014529:Uridine / Q000378:metabolism
D004247:DNA; D013936:Thymidine; D014529:Uridine