title: Organization, fine structure, and viability of the human adrenal medulla: considerations for neural transplantation.
abstract: Recent reports of adrenal medullary autografts in patients with Parkinson's disease raise several important questions with respect to the cell types actually being transplanted as well as the potential for chromaffin cell banking prior to neural transplantation. In this study, we determined the general morphological characteristics of the human adrenal medulla and assessed factors important for the maintenance of cultured chromaffin cells for later use as transplants. The human adrenal medulla contained islands of cortical cells scattered throughout the gland as well as Schwann cells, nerve endings, endothelial cells, pericytes, isolated ganglionic neurons, and connective tissue elements such as fibroblasts and smooth muscle cells. Because many of these cell types are mitotically active, transplantation of medullary fragments that contain these cells could have far-reaching consequences. One approach that could circumvent the problems arising from multiple cell types in the medulla is differential plating of chromaffin cells prior to transplantation. Differential plating yielded relatively pure populations of chromaffin cells that demonstrated excellent viability if processed within 2 hours after cessation of the gland's circulation. Chromaffin cells cultured in the presence of nerve growth factor exhibited a neuronal phenotype, possessed catecholamine histofluorescence, and displayed tyrosine hydroxylase- and dopamine beta-hydroxylase-like immunoreactivity. The sex and age of the donor did not affect cell viability or morphological characteristics.
authors.affiliation: Department of Neurobiology and Anatomy, University of Rochester School of Medicine and Dentistry, NY 14642.
authors.forename: J T
authors.initials: JT
authors.lastname: Hansen
authors.forename: M F
authors.initials: MF
authors.lastname: Notter
authors.forename: S H
authors.initials: SH
authors.lastname: Okawara
authors.forename: D M
authors.initials: DM
authors.lastname: Gash
mesh_terms: D000313:Adrenal Medulla / Q000502:physiology / Q000637:transplantation / Q000648:ultrastructure*; D002469:Cell Separation; D002478:Cells, Cultured; D002838:Chromaffin System / Q000502:physiology / Q000637:transplantation / Q000648:ultrastructure; D003470:Culture Media; D006801:Humans; D009414:Nerve Growth Factors / Q000494:pharmacology; D010300:Parkinson Disease / Q000601:surgery; D014022:Tissue Survival; D014182:Transplantation, Autologous
chemical_list: D003470:Culture Media; D009414:Nerve Growth Factors