New enzymatic determination of sialic acid in serum.
This enzymatic method for determination of sialic acid involves use of neuraminidase (EC 3.2.1.18), N-acetylneuraminate lyase (EC 4.1.3.3), acylglucosamine 2-epimerase (EC 5.1.3.8), N-acetylhexosamine oxidase (from Pseudomonas sp.), and peroxidase (EC 1.11.1.7). Because the method does not require pyruvic acid in the assay medium, interference by pyruvic acid in serum can be avoided. This simple, accurate assay is little affected by other substances in serum.
Teshima
S
S
Tsuruga Enzyme Plant, Toyobo Co. Ltd., Fukui, Japan.
Tamai
K
K
Hayashi
Y
Y
Emi
S
S
D002237:Carbohydrate Dehydrogenases / Q000378:metabolism; D002238:Carbohydrate Epimerases / Q000378:metabolism; D002352:Carrier Proteins; D006801:Humans; D008722:Methods; D019158:N-Acetylneuraminic Acid; D009439:Neuraminidase / Q000378:metabolism; D007652:Oxo-Acid-Lyases / Q000378:metabolism; D010544:Peroxidases / Q000378:metabolism; D011771:Pyruvate Oxidase / Q000378:metabolism; D012794:Sialic Acids / Q000097:blood*
D002352:Carrier Proteins; D012794:Sialic Acids; D002237:Carbohydrate Dehydrogenases; C057730:N-acetylhexosamine oxidase; D010544:Peroxidases; D011771:Pyruvate Oxidase; D009439:Neuraminidase; D007652:Oxo-Acid-Lyases; C018728:N-acetylneuraminate lyase; D002238:Carbohydrate Epimerases; C024519:N-acyl-D-glucosamine 2-epimerase; C498259:RENBP protein, human; D019158:N-Acetylneuraminic Acid