title: Evaluation of the contribution of tannin to the acute pulmonary inflammatory response against inhaled cotton mill dust.
abstract: Anesthetized, intubated, and mechanically ventilated rabbits were exposed to aerosolized saline, cotton dust extract (CDE), or tannin for 5 minutes and lavaged 4 hours after exposure. Cell numbers and types present in the bronchoalveolar lavage fluid (BALF) were determined and the concentrations of thromboxane A2 (TxA2) and prostaglandin F2-alpha (PGF2-alpha) in the BALF were also analyzed. The saline control animals had increased numbers and percentage of polymorphonuclear leukocytes (PMN) in the BALF as well as increased levels of TxB2 and PGF2-alpha compared with unexposed animals. Exposure to CDE further increased the number and percentage of PMN and the level of PGF2-alpha but had no effect on TxA2 levels when compared with control animals. Tannin exposure increased PGF2-alpha levels to the same extent as CDE exposure. PMN also increased but to a lesser extent than with CDE. These results indicate that the inflammatory response to CDE is only partially due to the tannin present in CDE.
authors.affiliation: Thoracic Diseases Research Unit, Mayo Clinic/Foundation, Rochester, Minnesota 55905.
authors.forename: D E
authors.initials: DE
authors.lastname: Lauque
authors.forename: S L
authors.initials: SL
authors.lastname: Hempel
authors.forename: M A
authors.initials: MA
authors.lastname: Schroeder
authors.forename: R E
authors.initials: RE
authors.lastname: Hyatt
authors.forename: M S
authors.initials: MS
authors.lastname: Rohrbach
mesh_terms: D000818:Animals; D002095:Byssinosis / Q000209:etiology* / Q000473:pathology; D002630:Chemotactic Factors / Q000032:analysis; D015237:Dinoprost / Q000032:analysis; D003368:Gossypium / Q000009:adverse effects*; D007249:Inflammation / Q000209:etiology; D008168:Lung / Q000032:analysis / Q000473:pathology*; D009504:Neutrophils; D011817:Rabbits; D012130:Respiratory Hypersensitivity / Q000209:etiology* / Q000473:pathology; D013634:Tannins / Q000009:adverse effects*; D007507:Therapeutic Irrigation; D013928:Thromboxane A2 / Q000032:analysis
chemical_list: D002630:Chemotactic Factors; D013634:Tannins; D013928:Thromboxane A2; D015237:Dinoprost