IMP synthesis using immobilized adenosine (phosphate) deaminase.
Inosinic acid (IMP) has been prepared by the deamination of adenosine monophosphate (AMP) with an immobilized adenosine (phosphate) deaminase extracted from the snail Biomphalaria glabrata. The enzyme has been immobilized in polyacrylamide beads. The preparation and characterization of this system are described.
Carvalho Júnior
L B
LB
Departamento de Bioquimica, Universidade Federal de Pernambuco, Cidade Universitaria, Recife, PE, Brasil.
Oliveira
E A
EA
Silva
M P
MP
Accioly
L G
LG
D000243:Adenosine Deaminase / Q000302:isolation & purification / Q000378:metabolism*; D000818:Animals; D001700:Biomphalaria / Q000201:enzymology; D004800:Enzymes, Immobilized / Q000302:isolation & purification / Q000378:metabolism*; D007291:Inosine Monophosphate / Q000096:biosynthesis*; D007292:Inosine Nucleotides / Q000096:biosynthesis*; D009700:Nucleoside Deaminases / Q000378:metabolism*
D004800:Enzymes, Immobilized; D007292:Inosine Nucleotides; D007291:Inosine Monophosphate; D009700:Nucleoside Deaminases; D000243:Adenosine Deaminase