Cellular distribution of protein-disulfide interchange enzyme activity in mouse spleen.
In an attempt to precise the cellular distribution of protein-disulfide interchange enzyme activity within different compartments of the splenic lymphoid tissue, we have analyzed the protein-disulfide interchange (PDI) enzyme activity in adherent and nonadherent cell populations of normal and T-cell depleted CBA mice. In vivo depletion of T-cells, as evaluated by functional and cytotoxic tests, was achieved by two i. v. injections of anti-T monoclonal antibodies (Mab F7D5). Nonadherent cell populations were found to have levels of protein-disulfide interchange enzyme activity significantly higher than that of the adherent cells. Pretreatment with F7D5 monoclonal antibodies enhanced the protein-disulfide interchange activity in nonadherent cell population, thus indicating that the major source of the enzyme activity are nonadherent spleen cells, which do not bear T-cell marker, probably B-cells.
Mimić-Oka
J I
JI
Institute of Biochemistry, School of Medicine, University of Belgrade, Yugoslavia.
Lukić
M L
ML
D000818:Animals; D002448:Cell Adhesion; D007535:Isomerases / Q000032:analysis*; D008221:Lymphoid Tissue / Q000201:enzymology; D051379:Mice; D008808:Mice, Inbred CBA; D019704:Protein Disulfide-Isomerases; D013154:Spleen / Q000201:enzymology*; D014018:Tissue Distribution
D007535:Isomerases; D019704:Protein Disulfide-Isomerases