Objective image alignment for three-dimensional reconstruction of digital autoradiograms.
Autoradiography can generate large quantities of information related to brain metabolism, blood flow, transport across the blood-brain barrier, neurotransmitter-receptor binding and other aspects of brain function. Three-dimensional (3D) reconstruction of digitized autoradiograms provides a mechanism for efficient analysis of function, in detail, over the entire brain. 3D reconstructions of the mean and variance can be obtained by superimposing data from similar experiments, leading ultimately to 3D reconstructions of differences with statistical tests of significance. Image registration is essential for reconstruction, and this article reports two independent algorithms for coronal image alignment that have been successfully implemented in computer programs. The first algorithm superimposes the centroids and principal axes of serial images; the extent and direction of the translation and rotation required for each image is obtained from an analysis of the inertia matrix of that image. The second algorithm matches the edges of structure features in serial-adjacent images, from analyses of the cross-correlation function of each pair of adjacent images. The cross-correlation method requires a great deal more computation than the principal axes method, but it can align damaged sections not reliably treated by the principal axes method. The methods are described in detail, and a quantitative assessment of the registration of non-identical images is considered.
Hibbard
L S
LS
Department of Radiology and Anesthesia, Milton S. Hershey Medical Center, Pennsylvania State University, Hershey 17033.
Hawkins
R A
RA
D000465:Algorithms; D000818:Animals; D001345:Autoradiography / Q000379:methods*; D001921:Brain / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D051381:Rats
D005947:Glucose