title: Californium-252 plasma desorption mass spectrometry of aminoglycoside antibiotics.
abstract: This paper presents mass spectral data of eleven aminoglycoside antibiotics by using californium-252 plasma desorption (252Cf PD) mass spectrometry. This mass spectral data could be used to develop a confirmatory method for monitoring aminoglycoside antibiotic residues isolated from food products of animal origin. Mass spectra were determined by applying time-of-flight 252Cf PD mass spectrometry to eleven aminoglycoside antibiotics, namely: neomycin, kanamycin, paromomycin, tobramycin, apramycin, streptomycin, dihydrostreptomycin, amikacin, netilmicin, sisomicin and gentamicins. All eleven antibiotics yielded positive ion spectra. These hydrophilic antibiotics were derivitized to extractable chromopheric compounds. All but two antibiotics (streptomycin and dihydrostreptomycin) yielded nitrophenyl derivatives and spectra were obtained in both negative and positive ion modes. Derivatized aminoglycosides produced cation and anion spectra with quasimolecular ions corresponding to [M + H]+., M+, [M - H]-., [M + Na]+, [M + K]+ and M-. or [M - H]- and M-. or [M - H]-. Underivatized antibiotics were best examined in the positive ion mode. 252Cf PD mass spectrometry consistently produced very strong molecular or quasimolecular ions for all aminoglycoside antibiotics.
authors.affiliation: Division of Production Drugs, US Food and Drug Administration, Rockville, Maryland 20857.
authors.forename: A H
authors.initials: AH
authors.lastname: Khan
authors.forename: B
authors.initials: B
authors.lastname: Shaikh
authors.forename: E H
authors.initials: EH
authors.lastname: Allen
authors.forename: E A
authors.initials: EA
authors.lastname: Sokoloski
mesh_terms: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents / Q000032:analysis*; D002142:Californium; D004350:Drug Residues / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods*
chemical_list: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D002142:Californium