title: Characterization and cDNA cloning of the pheromone-binding protein from the tobacco hornworm, Manduca sexta: a tissue-specific developmentally regulated protein.
abstract: cDNA encoding pheromone-binding protein (PBP), the major soluble protein in olfactory sensilla of male moths, has been cloned from the tobacco hornworm, Manduca sexta. A study of the developmental time course of PBP reveals that it is first synthesized just prior to eclosion and that the percentage of antennal mRNA encoding PBP shifts from zero to about 20% at that time. PBP is also found in sensilla from female M. sexta antennae. No amino acid sequence homology is observed between PBP and the vertebrate odorant-binding protein.
authors.affiliation: Section of Molecular Neurobiology, Howard Hughes Medical Institute Research Laboratories, Yale University School of Medicine, New Haven, CT 06510.
authors.forename: T K
authors.initials: TK
authors.lastname: Györgyi
authors.forename: A J
authors.initials: AJ
authors.lastname: Roby-Shemkovitz
authors.forename: M R
authors.initials: MR
authors.lastname: Lerner
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis*; D005260:Female; D007301:Insect Hormones / Q000235:genetics* / Q000378:metabolism; D019476:Insect Proteins; D007915:Lepidoptera; D008969:Molecular Sequence Data
chemical_list: D007301:Insect Hormones; D019476:Insect Proteins; C058053:pheromone-binding protein, Manduca sexta; D004247:DNA