Glucose elevates ornithine decarboxylase expression in Vero cells.
The addition of Earle's balanced salt solution (EBSS) of amino acids that are transported by a Na+-dependent cotransport system was not required by Vero cells for ornithine decarboxylase (ODC:EC 4.1.1.17) amplification. Vero cell ODC activity was elevated tenfold above basal levels when confluent cells were incubated for 5 hr in EBSS alone. ODC activity increased as a function of the incubation time in EBSS and was not elevated above basal enzyme levels when cells were incubated in EBSS minus glucose. ODC expression increased as a function of the glucose concentration in EBSS, with 20 mM glucose producing a 90-fold increase in ODC activity. ODC expression is more responsive to glucose in high-density quiescent cultures than in low-density growing cultures. Enhanced ODC expression by glucose depended on Na+ and K+ concentrations. The specific activity of ODC was also elevated above basal levels when mannose or fructose replaced glucose in EBSS. The addition of alanine or asparagine to EBSS enhanced ODC activity above levels obtained with EBSS containing standard (5.5 mM) glucose concentrations. In the absence of glucose, alanine was more effective than asparagine in enhancing ODC expression. These results suggest that the transport of amino acids is not an absolute requirement for Vero cell ODC expression and that ODC expression is linked to changes in cellular energetics and/or ion fluxes.
Lundgren
D W
DW
Department of Pediatrics, Case Western Reserve University School of Medicine, Cleveland, Ohio 44109.
Prokay
S L
SL
D000409:Alanine / Q000378:metabolism / Q000494:pharmacology; D000818:Animals; D001216:Asparagine / Q000494:pharmacology; D002452:Cell Count; D002455:Cell Division; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology*; D009955:Ornithine Decarboxylase / Q000096:biosynthesis* / Q000378:metabolism; D011188:Potassium / Q000378:metabolism / Q000494:pharmacology; D012964:Sodium / Q000494:pharmacology; D014709:Vero Cells
D001216:Asparagine; D012964:Sodium; D009955:Ornithine Decarboxylase; D005947:Glucose; D000409:Alanine; D011188:Potassium