[Application of simultaneous flow cytometric bromodeoxyuridine (BrdU)/DNA analysis. 2. Basic study to apply as a chemosensitivity test].
We developed a new method to rapidly measure the effectiveness of certain anticancer drugs by analyzing their effects on cell kinetics and cell cycle progression of labeled cells using simultaneous flow cytometric BrdU/DNA analysis. Three anticancer drugs, adriamycin (ADR), vincristine (VCR), and cisplatin (CDDP) were tested using the cultured cell line (MBT-2) originated from FANFT-induced mouse bladder tumor. The effects of these drugs were compared with percent colony survivals calculated by colony assay. The anticancer effects (IC90 levels) of ADR and CDDP could be determined by analyzing the effect of the drug on cell kinetics and cell cycle progression using the aforementioned method. However, in the case of VCR no relationship was found between the effect of the drug on cell kinetics and its anticancer effectiveness. Therefore, we must search for a better parameter for VCR, such as the inhibition rate of BrdU uptake.
Shimabukuro
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Department of Urology, Yamaguchi University School of Medicine.
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001973:Bromodeoxyuridine; D002453:Cell Cycle / Q000187:drug effects; D002945:Cisplatin / Q000494:pharmacology; D004247:DNA / Q000032:analysis*; D004317:Doxorubicin / Q000494:pharmacology; D004354:Drug Screening Assays, Antitumor / Q000379:methods; D005434:Flow Cytometry / Q000379:methods; D051379:Mice; D014407:Tumor Cells, Cultured; D001749:Urinary Bladder Neoplasms / Q000473:pathology; D014750:Vincristine / Q000494:pharmacology
D000970:Antineoplastic Agents; D014750:Vincristine; D004317:Doxorubicin; D004247:DNA; D001973:Bromodeoxyuridine; D002945:Cisplatin