Crystallization and preliminary X-ray analysis of type B influenza virus neuraminidase complexed with antibody Fab fragments.
Fab fragments from four different monoclonal antibodies have been complexed with influenza B virus neuraminidase (B/Lee/40) and the complexes have been crystallized. Three of the complex crystals are, so far, not suitable for X-ray diffraction studies, but the fourth (B/Lee/40 NA-B1Fab) forms large crystals which diffract X-rays to 3.0 A resolution. The crystals have a space group of F432, a = 441.21 A. Vm calculations show that the asymmetric unit contains two monomeric complexes.
Laver
W G
WG
John Curtin School of Medical Research, Australian National University, Canberra City.
Luo
M
M
Bossart
P J
PJ
Babu
Y S
YS
Smith
C
C
Accavitti
M A
MA
Tulloch
P A
PA
Air
G M
GM
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000936:Antigen-Antibody Complex; D000956:Antigens, Viral; D003460:Crystallization; D007140:Immunoglobulin Fab Fragments; D009981:Influenza B virus / Q000201:enzymology*; D008854:Microscopy, Electron; D009439:Neuraminidase / Q000276:immunology
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000936:Antigen-Antibody Complex; D000956:Antigens, Viral; D007140:Immunoglobulin Fab Fragments; D009439:Neuraminidase