title: Absorption and metabolism of orally fed arachidonic and linoleic acid in the rat.
abstract: [3H]arachidonic [( 3H]20:4) and [14C]linoleic acid [14C]18:2) were fed to rats in Intralipid or cream. Later (30-240 min) the stomach, small intestine, plasma, and liver were analyzed for radioactivity in different lipid classes. [3H]20:4 and [14C]18:2 were emptied from the stomach and absorbed by the intestine at similar rates. The [3H]20:4:[14C]18:2 ratio of the lipids in the small intestinal wall increased, however, with time. This was due to a higher retention of [3H]20:4 than [14C]18:2 in intestinal phospholipids. In contrast, more of the [14C]18:2 was in triacylglycerol of the small intestine and plasma. The highest 3H:14C ratios were found in phosphatidylethanolamine and phosphatidylinositol. The 3H:14C ratio of intestinal phosphatidylcholine varied with the type of fat vehicle used, being highest in the Intralipid experiments. After feeding Intralipid (30-60 min), significantly more of the plasma [3H]20:4 than plasma [14C]18:2 was in diacylglycerol, the 3H:14C ratio of which was much higher than that of plasma free fatty acids. [3H]20:4 and [14C]18:2 of chyle triacylglycerol are thus metabolized differently.
authors.affiliation: Department of Medicine, University of Lund, Sweden.
authors.forename: A
authors.initials: A
authors.lastname: Nilsson
authors.forename: T
authors.initials: T
authors.lastname: Melin
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001095:Arachidonic Acids / Q000008:administration & dosage / Q000097:blood / Q000378:metabolism*; D005746:Gastric Emptying; D005753:Gastric Mucosa / Q000378:metabolism; D007408:Intestinal Absorption; D007421:Intestine, Small / Q000378:metabolism; D008041:Linoleic Acids / Q000008:administration & dosage / Q000097:blood / Q000378:metabolism*; D050356:Lipid Metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D009092:Mucous Membrane / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D001095:Arachidonic Acids; D008041:Linoleic Acids