Phosphatidylethanol formation in human platelets: evidence for thrombin-induced activation of phospholipase D.
Phosphatidylethanol formation was examined in (3H)arachidonic acid-labeled human platelets. In the presence of ethanol, thrombin induced the formation of (3H)phosphatidylethanol in a time- and concentration-dependent manner. The appearance of phosphatidylethanol did not involve de novo synthesis since no 32P radioactivity was incorporated into phosphatidylethanol in 32P-labeled platelets or in platelets that were permeabilized with saponin in the presence of 32P-ATP. The data provide evidence for the direct activation by thrombin of phospholipase D in human platelets.
Rubin
R
R
Department of Pathology and Cell Biology, Jefferson Medical College, Thomas Jefferson University, Philadelphia, Pennsylvania 19107.
D000328:Adult; D001792:Blood Platelets / Q000378:metabolism*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation; D000431:Ethanol / Q000494:pharmacology; D020404:Glycerophospholipids; D006801:Humans; D010712:Phosphatidic Acids / Q000096:biosynthesis* / Q000097:blood; D010739:Phospholipase D / Q000097:blood*; D010740:Phospholipases / Q000097:blood*; D013917:Thrombin / Q000494:pharmacology*
D020404:Glycerophospholipids; D010712:Phosphatidic Acids; C051521:phosphatidylethanol; D000431:Ethanol; D010740:Phospholipases; D010739:Phospholipase D; D013917:Thrombin