Use of avidin-coated glass beads as a support for high-performance immunoaffinity chromatography.
Immunoaffinity chromatography, using immobilized antibodies, is a useful technique for the specific isolation and purification of biological materials. We have developed an avidin-coated glass bead which can easily immobilize and retain under adverse conditions, such as acid elution, any antibody which is covalently labelled with biotin. The physical strength of the bead, together with the expanding repertoire of commercially available biotinylated monoclonal antibodies makes this support ideal for both medium- and high-performance immunoaffinity chromatography.
Babashak
J V
JV
Kontes Scientific Glassware, Vineland, NJ.
Phillips
T M
TM
D000911:Antibodies, Monoclonal / Q000276:immunology; D000937:Antigen-Antibody Reactions; D000941:Antigens / Q000302:isolation & purification; D001360:Avidin; D002846:Chromatography, Affinity / Q000295:instrumentation*; D005898:Glass; D007120:Immunochemistry / Q000295:instrumentation*
D000911:Antibodies, Monoclonal; D000941:Antigens; D001360:Avidin