Differential phosphorylation of murine class I major histocompatibility antigens.
Recent studies have shown that the H-2K and H-2D transplantation antigens are expressed differentially in different tissues of mouse. Our previous investigations also established that in thioglycolate-stimulated peritoneal macrophages the H-2Dk antigen exists in distinct cell surface and intracellular forms. These two forms are glycosylated differently. In this report, we have found that (1) H-2Dk antigen is phosphorylated whereas H-2Kk antigen is not, and (2) only the cell surface form of H-2Dk antigen is phosphorylated in thioglycolate-stimulated macrophages derived from C3H/Heha mice. This differential phosphorylation of H-2 antigens will provide a model system for further studies on the molecular mechanism and function of phosphorylation of H-2 antigens.
Hong
W J
WJ
Department of Biological Science, State University of New York, Buffalo 14260.
Van Le
A
A
Doyle
D
D
D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional; D015395:Histocompatibility Antigens Class I / Q000032:analysis / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008815:Mice, Inbred Strains; D010766:Phosphorylation; D011233:Precipitin Tests
D015395:Histocompatibility Antigens Class I