[Microinjection of spermatozoa into the oocyte. An experimental model for assisted conjugation in oligo-asthenozoospermia as a cause of sterility].
This study investigates the possibilities to treat oligo-asthenozoospermia, a cause of sterility, by assisted conjugation. After completing acrosome reaction, normal motile spermatozoa are microinjected into mouse oocytes. After 2 hours of incubation time in medium with lactate and albumins, spermatozoa are put into dibutyryl 3',5'-guanosine monophosphate (dbcGMP) and imidazole for the completion of an acrosome reaction. One motile spermatozoon each was injected microsurgically into the perivitelline space of an oocyte. The survival rate of the oocytes after injection was around 85%. The oocytes with two pronuclei and a second polar body were considered as fertilized. Of those injected oocytes 21.5% were fertilized with acrosome reacted spermatozoa, whereas only 5.6% were fertilized with spermatozoa without dbcGMP treatment. Thus, motile and acrosome reacted spermatozoa essentially fertilize oocytes at microinjection into the perivitelline space very well. This technique may become a hopeful procedure to treat human oligo-asthenozoospermia in an IVF/ET-programme.
Mettler
L
L
Abteilung Frauenheilkunde im Zentrum für Operative Medizin I der Christian-Albrechts-Universität, Kiel.
Yamada
K
K
Kuranty
A
A
D000818:Animals; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D007267:Injections; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008866:Microsurgery / Q000379:methods*; D009845:Oligospermia / Q000628:therapy*; D013075:Sperm Capacitation; D013084:Sperm-Ovum Interactions; D014817:Vitelline Membrane
Mikroinjektion von Spermatozoen in die Eizelle. Ein experimentelles Modell zur assistierten Konjugation bei Oligo-Asthenozoospermie als Sterilitätsursache.