Use of the MTT assay for rapid determination of chemosensitivity of human leukemic blast cells.
A microcytotoxicity assay employing a tetrazolium salt has been adapted for testing the response of human leukemic blast cells to a variety of chemotherapeutic agents. After exposure to various concentrations of drugs, the viability of fresh leukemic blast cells was measured using a tetrazolium salt, MTT, which is converted to blue formazan crystals by living cells. The amount of formazan produced was quantitated using a microtitre plate spectrophotometer. In the present study, optimal conditions for chemosensitivity testing of human leukemia samples were determined, and the relative chemosensitivity of five patient samples was tested.
Campling
B G
BG
Department of Oncology, Queen's University, Kingston, Ontario, Canada.
Pym
J
J
Galbraith
P R
PR
Cole
S P
SP
D000970:Antineoplastic Agents / Q000494:pharmacology* / Q000627:therapeutic use; D001752:Blast Crisis / Q000188:drug therapy / Q000473:pathology*; D002470:Cell Survival; D004351:Drug Resistance; D005562:Formazans / Q000032:analysis; D006801:Humans; D008832:Microchemistry; D014411:Neoplastic Stem Cells / Q000187:drug effects*; D013053:Spectrophotometry; D013778:Tetrazolium Salts; D013844:Thiazoles; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000970:Antineoplastic Agents; D005562:Formazans; D013778:Tetrazolium Salts; D013844:Thiazoles; C055223:nitroblue formazan; C022616:thiazolyl blue