Characterization of a sequence of human T cell leukemia virus type I from a patient with chronic progressive myelopathy.
DNA from peripheral blood mononuclear cells of individuals with chronic progressive myelopathy (CPM) were extensively analyzed for the presence of human T cell leukemia virus (HTLV) type I-like sequences by using the polymerase chain reaction. The DNA samples were amplified with oligonucleotides from three separate regions of HTLV viral genomes. A portion of the amplified viral genome from a representative patient was sequenced after molecular cloning into bacteriophage M13. Sequence data indicate that HTLV type I and not a related virus is associated with CPM.
Kwok
S
S
Department of Infectious Diseases, Cetus Corporation, Emeryville, California 94608.
Kellogg
D
D
Ehrlich
G
G
Poiesz
B
B
Bhagavati
S
S
Sninsky
J J
JJ
D000368:Aged; D001483:Base Sequence; D003001:Cloning, Molecular; D015342:DNA Probes; D004279:DNA, Viral / Q000032:analysis* / Q000235:genetics; D005260:Female; D005784:Gene Amplification; D015368:Human T-lymphotropic virus 1 / Q000235:genetics*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000382:microbiology; D008969:Molecular Sequence Data; D015493:Paraparesis, Tropical Spastic / Q000097:blood / Q000382:microbiology*
D015342:DNA Probes; D004279:DNA, Viral