[The somatic transformation of hens using high-polymeric DNA].
The transformation studies were performed on genetically pure hen breeds: the donor was Russian white (white colour is a dominant character), the recipient was New Hampshire (pale-yellow-brown). The high-polymeric DNA (2.10(9) dalton) was isolated from erythrocytes and testicles of donor cocks and introduced to a fresh-laid egg of the recipient by replacing the protein. Two sets of experiments on 570 eggs were made. The single injection with 150 micrograms of donor DNA to the recipient egg induced a stable change of feathering colour (particoloured) in 46% of the hatched chicks, which remained for 6 months of observation. Thus, the high-polymeric DNA possesses the property of somatic transformation, that evidences for a high intact character of these preparations.
Strazhevskaia
N B
NB
Struchkov
V A
VA
D000818:Animals; D001947:Breeding; D002645:Chickens / Q000235:genetics*; D003116:Color; D004247:DNA / Q000235:genetics / Q000494:pharmacology*; D005241:Feathers; D005260:Female; D008297:Male; D008970:Molecular Weight; D010641:Phenotype; D012641:Selection, Genetic; D014170:Transformation, Genetic / Q000187:drug effects*
D004247:DNA
Somaticheskaia transformatsiia kur s pomoshch'iu vysokopolimernoĭ DNK.