title: Contribution of polycyclic aromatic compounds to the carcinogenicity of sidestream smoke of cigarettes evaluated by implantation into the lungs of rats.
abstract: Particles and semivolatiles from sidestream smoke of cigarettes smoked on a smoking machine were collected by a filter combination consisting of a glass fibre filter and silanized polystyrene beads. The extract of the glass fibre filter was separated by a Sephadex LH-20 column chromatography into a fraction containing non-aromatic material plus polycyclic aromatic compounds (PAC) with 2 and 3 rings and a fraction consisting of PAC with 4 and more rings. To evaluate the carcinogenicity, both fractions as well as the semivolatiles were implanted into the lungs of Osborne-Mendel rats at a dose level of one cigarette per animal and compared with three dose levels of benzo[a]pyrene (BaP). The most pronounced carcinogenic effect of the sidestream smoke (100 ng BaP per cigarette) was caused by the fraction containing polycyclic aromatic hydrocarbons (PAH) with 4 and more rings (5 carcinomas of the lungs/35 rats). This fraction represents only 3.5% by weight of the total sidestream smoke condensate. By contrast, the semivolatile material did not provoke any tumors. Only a small contribution to the total carcinogenicity (1 carcinoma of the lungs/35 rats) was observed for the fraction containing non-aromatic material and 2- and 3-ring PAHs.
authors.affiliation: Biological Institute for Environmental Carcinogens, Grosshansdorf, F.R.G.
authors.forename: G
authors.initials: G
authors.lastname: Grimmer
authors.forename: H
authors.initials: H
authors.lastname: Brune
authors.forename: G
authors.initials: G
authors.lastname: Dettbarn
authors.forename: K W
authors.initials: KW
authors.lastname: Naujack
authors.forename: U
authors.initials: U
authors.lastname: Mohr
authors.forename: R
authors.initials: R
authors.lastname: Wenzel-Hartung
mesh_terms: D000818:Animals; D001564:Benzo(a)pyrene / Q000633:toxicity; D003043:Cocarcinogenesis; D005260:Female; D008175:Lung Neoplasms / Q000139:chemically induced*; D010947:Plants, Toxic; D011083:Polycyclic Compounds / Q000633:toxicity*; D051381:Rats; D011919:Rats, Inbred Strains; D012906:Smoke / Q000009:adverse effects*; D014026:Nicotiana
chemical_list: D011083:Polycyclic Compounds; D012906:Smoke; D001564:Benzo(a)pyrene