Increased elastin mRNA levels associated with surgically induced intimal injury.
Quantitative levels of mRNAs coding for elastin, types I and III procollagen and gamma-actin were measured in porcine vascular material following balloon catheterization. A balloon catheter was introduced into the thoracic aorta and jugular vein of 3-6 week old pigs; following distention and six days of postoperative recovery, tissue samples were obtained for histopathology, electron microscopy, RNA extraction and mRNA quantitation. Using a series of mammalian cDNA clones and the procedure of slot blot hybridization, we have shown that elastin and types I and III procollagen mRNA levels rose significantly during the postoperative period following vascular distention. The increase correlated with an increase in the cell mass present in both the venous and arterial intimal layers. Changes in gamma-actin mRNA levels were also associated with this rapid proliferative response but in arterial tissue only.
Boyd
C D
CD
Department of Surgery, UMDNJ-Robert Wood Johnson Medical School, New Brunswick 08903.
Kniep
A C
AC
Pierce
R A
RA
Deak
S B
SB
Karboski
C
C
Miller
D C
DC
Parker
M I
MI
Mackenzie
J W
JW
Rosenbloom
J
J
Scott
G E
GE
D000199:Actins / Q000096:biosynthesis; D000818:Animals; D015152:Blotting, Northern; D004247:DNA / Q000378:metabolism; D004549:Elastin / Q000096:biosynthesis*; D004730:Endothelium, Vascular / Q000378:metabolism* / Q000473:pathology; D006965:Hyperplasia / Q000378:metabolism / Q000473:pathology; D011347:Procollagen / Q000096:biosynthesis; D012333:RNA, Messenger / Q000378:metabolism*; D013314:Stress, Mechanical; D013552:Swine
D000199:Actins; D011347:Procollagen; D012333:RNA, Messenger; D004247:DNA; D004549:Elastin