title: Differential effects of p-nitrophenyl-D-xylosides on mouse blastocysts and uterine epithelial cells.
abstract: A number of studies have implicated glycoconjugates in cell recognition events associated with implantation of mammalian blastocysts into the uterus. We have found that p-nitrophenyl-D-xylosides inhibit mouse embryo attachment and outgrowth on monolayers of uterine epithelial cells when cocultured in vitro. Inhibition of attachment and trophoblast formation by alpha- and beta-xylosides was observed in embryos cultured on tissue culture plastic in serum containing medium or on monolayers of epithelial cells. The biochemical basis for this inhibition has been investigated. Consistent with their accepted mode of action, beta- but not alpha-D-xylosides greatly stimulated glycosaminoglycan chain production by uterine epithelial cells and likewise reduced proteoglycan assembly. In contrast, both alpha- and beta-anomers selectively inhibited embryo attachment and outgrowth without stimulating glycosaminoglycan chain production by embryos. The inhibitory effect of the xylosides on embryos was reversible and did not require concentrations that reduced the rate of protein synthesis. Both alpha- and beta-D-xylosides inhibited the synthesis of proteoglycans including heparan sulfate as well as certain other glycoconjugates by embryos. Collectively, these data indicate that proper assembly of glycoconjugates, including proteoglycans, is required for implantation-related processes, although the inhibition of embryo outgrowth by xylosides may be by an as yet uncharacterized mechanism.
authors.affiliation: Department of Physiology and Molecular Biophysics, Baylor College of Medicine, Houston, Texas.
authors.forename: M C
authors.initials: MC
authors.lastname: Farach
authors.forename: J P
authors.initials: JP
authors.lastname: Tang
authors.forename: G L
authors.initials: GL
authors.lastname: Decker
authors.forename: D D
authors.initials: DD
authors.lastname: Carson
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000187:drug effects*; D002478:Cells, Cultured; D046508:Culture Techniques; D010064:Embryo Implantation; D004848:Epithelium / Q000187:drug effects; D005260:Female; D006025:Glycosaminoglycans / Q000096:biosynthesis; D006027:Glycosides / Q000633:toxicity*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D014599:Uterus / Q000187:drug effects*
chemical_list: D006025:Glycosaminoglycans; D006027:Glycosides; C008646:4-nitrophenyl beta-D-xyloside