title: Location of a region of the muscarinic acetylcholine receptor involved in selective effector coupling.
abstract: Chimaeric muscarinic acetylcholine receptors (mAChR) in which corresponding portions of mAChR I and mAChR II are replaced with each other have been produced in Xenopus oocytes by expression of cDNA constructs encoding them. Functional analysis of the chimaeric mAChRs indicates that a region mostly comprising the putative cytoplasmic portion between the proposed transmembrane segments V and VI is involved in selective coupling of mAChR I and mAChR II with different effector systems. In contrast, the exchange of this region between mAChR I and mAChR II does not significantly affect the antagonist binding properties of the two mAChR subtypes.
authors.affiliation: Department of Medical Chemistry, Kyoto University Faculty of Medicine, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Kubo
authors.forename: H
authors.initials: H
authors.lastname: Bujo
authors.forename: I
authors.initials: I
authors.lastname: Akiba
authors.forename: J
authors.initials: J
authors.lastname: Nakai
authors.forename: M
authors.initials: M
authors.lastname: Mishina
authors.forename: S
authors.initials: S
authors.lastname: Numa
mesh_terms: D000818:Animals; D001483:Base Sequence; D002678:Chimera; D004247:DNA / Q000235:genetics*; D005260:Female; D007700:Kinetics; D008969:Molecular Sequence Data; D009865:Oocytes / Q000378:metabolism; D010890:Pirenzepine / Q000494:pharmacology; D011485:Protein Binding; D011976:Receptors, Muscarinic / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D013552:Swine; D014981:Xenopus
chemical_list: D011976:Receptors, Muscarinic; D010890:Pirenzepine; D004247:DNA