Immunohistological characterization of a Ewing's sarcoma case.
The histogenesis of Ewing's sarcoma (ES) remains uncertain. Mesenchymal and neuroectodermal origins were the most recent hypotheses. In an attempt to test these two hypotheses, frozen sections of an ES with the chromosomal translocation t(11;22) have been studied using a panel of antibodies directed against monocytes/macrophages cell surface antigens (Leu M1, Leu M2, Leu M3, and MO1), and against neural components (NSE, S-100, T4, and HNK-1). None of these antigens were detected. Positive reactions were obtained with antibodies recognizing HLA II antigen and B2-microglobulin. From a panel of various intermediate filaments only vimentin was shown to be present. None of the two hypotheses could be supported by the results obtained from the immunohistological analysis of the tumor studied. In the absence of a specific immunological pattern, the chromosomal t(11;22)(q24;q12) marker remains the only diagnostic criterion of ES.
Lizard-Nacol
S
S
Laboratory of Cytogenetics, Faculté de Médecine, Dijon, France.
Mugneret
F
F
Turc-Carel
C
C
Justrabo
E
E
D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation / Q000032:analysis; D002880:Chromosomes, Human, Pair 11; D006801:Humans; D007150:Immunohistochemistry; D012512:Sarcoma, Ewing / Q000473:pathology*; D014178:Translocation, Genetic
D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation