Acylation of lysophosphatidylcholine in liver microsomes of thermally acclimated trout.
The specificity of acyl-coenzyme A (CoA): lysophosphatidylcholine acyltransferase (LPCAT; EC 2.3.1.23) was determined for a range for acyl-CoA substrates differing with respect to chain length and degree of unsaturation in liver microsomes of thermally acclimated (5 and 20 degrees C) rainbow trout, Salmo gairdneri. Absolute levels of oleate incorporation into phosphatidylcholine (PC) were determined at substrate concentrations in the physiological range (12 microM) and higher (64 microM). The specificity of LPCAT was determined by the extent to which competing substrates decreased the incorporation of oleate. LPCAT specificity was significantly influenced by both assay and acclimation temperature at total substrate concentrations of both 72 and 256 microM. A clear preference for 14- and 16-carbon monoenes was exhibited by LPCAT from 20 but not 5 degrees C-acclimated trout. Furthermore, LPCAT from 5 degrees C-acclimated trout preferentially incorporated long chain and polyunsaturated fatty acids (eicosadienoyl, arachidonoyl, erucoyl) and excluded 18-carbon unsaturates at an assay temperature of 5 degrees C compared with 20 degrees C; at 20 degrees C, 18-carbon unsaturates were incorporated more readily than 20-carbon species. Linolenic acid (18:3N3) was generally excluded from incorporation, reflecting a possible mechanism by which this precursor of docasahexaenoic acid (22:6N3, n - 3) remains available for modification. These results indicate that trout liver LPCAT preferentially incorporates fatty acids into PC on the basis of both chain length and degree of unsaturation in a manner consistent with the temperature-induced restructuring of membrane phospholipids.
Livermore
R C
RC
Department of Zoology, Arizona State University, Tempe 85287.
Hazel
J R
JR
D000064:Acclimatization; D000214:Acyl Coenzyme A / Q000378:metabolism; D000215:Acylation; D000818:Animals; D003080:Cold Temperature; D008244:Lysophosphatidylcholines / Q000378:metabolism*; D008862:Microsomes, Liver / Q000378:metabolism*; D012016:Reference Values; D014337:Trout
D000214:Acyl Coenzyme A; D008244:Lysophosphatidylcholines; C017585:oleoyl-coenzyme A