Induction of specific-locus mutations in female mice by 1-ethyl-1-nitrosourea and procarbazine.
Using the specific-locus method, the ability of 1-ethyl-1-nitrosourea (ENU) and procarbazine hydrochloride (procarbazine) to induce gene mutations in mouse oocytes was tested and confirmed. The sensitive stage for the induction of mutations in oocytes with 160 mg/kg of ENU is 2-4 weeks post treatment. The induced mutation frequency in this mating interval was 5.1 X 10(-7) mutations/locus/gamete/mg/kg. The induction of mutations by procarbazine occurred 8-33 weeks after treatment. The induced mutation frequency in this mating interval for the 400 mg/kg group was 0.4 X 10(-7) mutations/locus/gamete/mg/kg. One third of the induced mutations was lethal in homozygous condition in both experiments. ENU and procarbazine have a lower mutational response in oocytes than in stem-cell spermatogonia.
Ehling
U H
UH
Institut für Säugetiergenetik, Gesellschaft für Strahlen- und Umweltforschung, Neuherberg, F.R.G.
Neuhäuser-Klaus
A
A
D000818:Animals; D004305:Dose-Response Relationship, Drug; D005038:Ethylnitrosourea / Q000494:pharmacology*; D005260:Female; D051379:Mice; D009030:Mosaicism; D009154:Mutation / Q000187:drug effects*; D009865:Oocytes / Q000187:drug effects*; D011344:Procarbazine / Q000494:pharmacology*
D011344:Procarbazine; D005038:Ethylnitrosourea