Metabolic energy production during adrenergic pH regulation in red cells of the Atlantic salmon, Salmo salar.
Whole blood from Atlantic salmon was incubated anaerobically at 10 degrees C so as to measure the metabolic activity of the nucleated erythrocytes. An acute extracellular acidosis was produced by adding either an acid solution (sham) or an acid solution with adrenaline (final concentration, 5 x 10(-4) M). The extracellular acidosis produced by the sham solution was transferred to the erythrocytes, whereas with adrenaline, intracellular pH actually increased in the face of a plasma acidosis. Indeed, the extracellular acidosis in the adrenaline-treated blood was significantly higher than that of the sham as a result of net H+ excretion from the erythrocyte. This pH response of the erythrocyte was accompanied by a proportional increase in the O2 consumption of the blood, with no change in lactate production. In comparison to sham-treated cells, the content of erythrocytic nucleotide triphosphates initially decreased upon addition of adrenaline but was thereafter maintained at a constant NTP/Hb ratio presumably due to an increased ATP turnover. In conclusion, it appears that the aerobic rather than anaerobic metabolism of erythrocytes is accelerated upon addition of adrenaline to blood, and that this increased metabolism is involved in fueling the membrane transport processes involved in adrenergic pH regulation of salmonid red cells.
Ferguson
R A
RA
Department of Biology, Dalhousie University, Halifax, Nova Scotia, Canada.
Boutilier
R G
RG
D000136:Acid-Base Equilibrium; D000227:Adenine Nucleotides / Q000032:analysis; D000818:Animals; D001692:Biological Transport; D004734:Energy Metabolism; D004837:Epinephrine / Q000494:pharmacology; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000378:metabolism*; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism*; D006400:Hematocrit; D006454:Hemoglobins / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D007773:Lactates / Q000378:metabolism; D010101:Oxygen Consumption; D012474:Salmon / Q000097:blood*
D000227:Adenine Nucleotides; D006454:Hemoglobins; D007773:Lactates; D004837:Epinephrine