Sustained viability of acute myelogenous leukemia cells in liquid suspension culture correlates with occurrence of differentiation.
To examine the relationship between viability of acute myelogenous leukemia (AML) blasts in liquid suspension culture and the occurrence of myeloid maturation in culture, total and differential cell counts and viability as determined by trypan blue dye exclusion were followed over 21 days of culture. A significant positive correlation was found between viability of cells on day 21 of culture and percentage of mature cells present. These data suggest that myeloid maturation may be a mechanism by which AML cells maintain viability in an adverse environment.
Baer
M R
MR
Department of Hematologic Oncology, Roswell Park Memorial Institute, Buffalo, NY 14263.
Gao
X Z
XZ
Preisler
H D
HD
D002454:Cell Differentiation; D002470:Cell Survival; D003470:Culture Media; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000473:pathology*; D007958:Leukocyte Count; D014411:Neoplastic Stem Cells / Q000473:pathology; D013535:Suspensions; D014407:Tumor Cells, Cultured / Q000473:pathology*
D003470:Culture Media; D013535:Suspensions