The interpretation of ELISA results by means of the standard deviation ratio.
An improved sensitivity to low levels of hepatitis B surface antigen (HBsAg) was demonstrated when ELISA results were interpreted by a statistical method. The absorbance of each test well was compared with the mean absorbance of all low-colour test samples. Those wells with an absorbance of more than two standard deviations above the mean were referred for confirmatory tests. Samples containing 1.0 ng/ml of HBsAg were reliably detected by a rapid assay technique (1.5 h). Predictive value analysis showed greater sensitivity than was possible from a direct comparison of test samples with controls.
Parkinson
R M
RM
Natal Blood Transfusion Service, Pinetown, Republic of South Africa.
Conradie
J D
JD
Milner
L V
LV
Marimuthu
T
T
D004797:Enzyme-Linked Immunosorbent Assay / Q000592:standards*; D005188:False Negative Reactions; D005189:False Positive Reactions; D006514:Hepatitis B Surface Antigens / Q000032:analysis*; D006801:Humans; D011237:Predictive Value of Tests; D012015:Reference Standards; D012016:Reference Values
D006514:Hepatitis B Surface Antigens