title: Preparation and structural characterization of nucleosomal core particles lacking one H2A.H2B dimer.
abstract: Nucleosomal core particles lacking one H2A.H2B dimer, (H2A.H2B)1 (H3.H4)2/DNA (146 bp), have been prepared by treatment of nucleosomal cores with dimethylmaleic anhydride, a reversible reagent for protein amino groups. The preparative procedure is simple, produces quantitative conversion of nucleosomal cores into dimer-deficient cores without formation of other subnucleosomal particles, and can be applied to the preparation of different H2A.H2B-deficient mono and oligonucleosomal particles. The structural properties of the dimer-deficient cores and complete nucleosomal cores reconstituted from the deficient particles and H2A.H2B dimers have been studied by DNase I digestion, thermal denaturation and circular dichroism.
authors.affiliation: Centro de Biología Molecular, Universidad Autónoma de Madrid, Cantoblanco, Spain.
authors.forename: S
authors.initials: S
authors.lastname: de la Escalera
authors.forename: M A
authors.initials: MA
authors.lastname: Nieto
authors.forename: E
authors.initials: E
authors.lastname: Palacián
mesh_terms: D000818:Animals; D002498:Centrifugation; D002645:Chickens; D002942:Circular Dichroism; D006657:Histones / Q000502:physiology*; D006358:Hot Temperature; D066298:In Vitro Techniques; D008299:Maleic Anhydrides / Q000494:pharmacology; D009707:Nucleosomes / Q000187:drug effects / Q000648:ultrastructure*
chemical_list: D006657:Histones; D008299:Maleic Anhydrides; D009707:Nucleosomes; C007474:2,3-dimethylmaleic anhydride