title: The Azorhizobium caulinodans nifA gene: identification of upstream-activating sequences including a new element, the 'anaerobox'.
abstract: From nucleotide sequencing analyses, the A. caulinodans nifA gene seems to be under dual control by the Ntr (in response to available N) and Fnr (in response to available O2) transcriptional activation/repression systems. Because it fixes N2 in two contexts, the Ntr system might regulate A. caulinodans nif gene expression ex planta, while the Fnr system might similarly regulate in planta. As nifA upstream-activating elements, we have identified: (i) a gpNifA binding site allowing autogenous nifA regulation, (ii) an Ntr-dependent transcription start, presumably the target of gpNifA activation, and (iii) an "anaerobox" tetradecameric nucleotide sequence that is precisely conserved among O2 regulated enteric bacterial genes controlled by the gpFnr transcriptional activator. Because it is precisely positioned upstream of enteric bacterial transcriptional starts, the "anaerobox" sequence may constitute the gpFnr DNA binding site. If so, then a second, Ntr-independent nifA transcription start may exist. We have also deduced the A. caulinodans nifA open reading frame and have compared the gene product (gpNifA) with those of other N2-fixing organisms. These proteins exhibit strongly conserved motifs: (i) sites conserved among ATP-binding proteins, (ii) an interdomain linker region, and (iii) a C-terminal alpha-helix-turn-alpha-helix DNA binding site.
authors.affiliation: Department of Biology, University of California, Santa Cruz 95064.
authors.forename: D W
authors.initials: DW
authors.lastname: Nees
authors.forename: P A
authors.initials: PA
authors.lastname: Stein
authors.forename: R A
authors.initials: RA
authors.lastname: Ludwig
mesh_terms: D000595:Amino Acid Sequence; D000693:Anaerobiosis; D001426:Bacterial Proteins / Q000235:genetics* / Q000302:isolation & purification; D001483:Base Sequence; D004268:DNA-Binding Proteins / Q000235:genetics; D005786:Gene Expression Regulation; D005798:Genes, Bacterial; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009586:Nitrogen Fixation / Q000235:genetics*; D012230:Rhizobiaceae / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
chemical_list: D001426:Bacterial Proteins; D004268:DNA-Binding Proteins