title: Simple radioimmunobinding assay for quantitation of sperm antibodies of IgG immunoglobulin class.
abstract: The immunobead test (IBT) is an excellent test for initial sperm antibody screening and evaluation but is impractical when used to quantitate antibody levels using a twofold dilution series. The aim of this study, therefore, was to develop a relatively simple radioimmunobinding assay that would allow quantitation of sperm antibodies of IgG immunoglobulin class, which predominate in male sera. [125I]-Protein G was chosen as the radioligand because it binds to all IgG subclasses, but not to IgA or IgM. The results of the investigation indicate that the [125I]-protein G assay (PGA) allows efficient quantitation of sperm antibodies, as evidenced by a highly significant (P less than .0001) correlation (Spearman's, Rs = 0.94) between the PGA results and IgG-IBT titres.
authors.affiliation: Department of Pathology, Royal Women's Hospital, Carlton, Australia.
authors.forename: G N
authors.initials: GN
authors.lastname: Clarke
mesh_terms: D001323:Autoantibodies / Q000032:analysis* / Q000592:standards; D001426:Bacterial Proteins / Q000592:standards; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis* / Q000145:classification / Q000592:standards; D007552:Isotonic Solutions; D008297:Male; D008863:Microspheres; D011863:Radioimmunoassay / Q000379:methods / Q000592:standards; D011869:Radioligand Assay / Q000379:methods / Q000592:standards; D015203:Reproducibility of Results; D012661:Semen / Q000276:immunology; D012710:Serum Albumin, Bovine; D013094:Spermatozoa / Q000276:immunology*; D013291:Streptococcus
chemical_list: D001323:Autoantibodies; D001426:Bacterial Proteins; C034284:IgG Fc-binding protein, Streptococcus; D007074:Immunoglobulin G; D007552:Isotonic Solutions; C037692:Tyrode's solution; D012710:Serum Albumin, Bovine