A gonial mitosis-stimulating factor in cerebral ganglia and hemolymph of the marine mussel Mytilus edulis L.
A gonial mitosis-stimulating factor produced by the cerebral ganglia of the mussel Mytilus edulis has been demonstrated. This factor induces an increase of [3H]thymidine incorporation in the DNA of isolated mantle cells. Dose-response data are obtained with the methanol phase of an acidic ganglia extract. The rate of [3H]thymidine incorporation is compared with aspartate transcarbamylase specific activity, another bioassay previously described to estimate mitotic activity. This heat-stable mitogenic factor appears to have a molecular weight of less than 5000 Da. A gonial mitogenic factor is also found in the hemolymph and circulatory cells.
Mathieu
M
M
Laboratoire de Zoologie, Université de Caen, France.
Lenoir
F
F
Robbins
I
I
D000818:Animals; D001221:Aspartate Carbamoyltransferase / Q000378:metabolism; D001681:Biological Assay; D001923:Brain Chemistry; D002850:Chromatography, Gel; D004247:DNA / Q000096:biosynthesis; D005724:Ganglia / Q000032:analysis*; D005854:Germ Cells / Q000166:cytology* / Q000378:metabolism; D006133:Growth Substances / Q000032:analysis / Q000494:pharmacology*; D006458:Hemolymph / Q000032:analysis*; D008938:Mitosis; D008970:Molecular Weight; D008974:Mollusca / Q000378:metabolism*; D012621:Seasons
D006133:Growth Substances; D004247:DNA; D001221:Aspartate Carbamoyltransferase