title: Cultured fetal tectal tissue grafted to the midbrain of newborn rats: morphology of grafts and innervation by host retinal and cortical axons.
abstract: It has been shown previously that tectal tissue obtained from young embryos can be successfully transplanted to the neonatal rat brain. In the present study, tecta from E15 rat embryos were maintained as free-floating explants for 3-14 days in vitro (DIV) before being transplanted to the midbrain of newborn rats. We wished to determine how explant culture affected (i) graft survival, (ii) the subsequent morphological and histochemical development of tectal grafts and (iii) the specificity with which host retinal and cortical axons grew into and innervated the graft neuropil. Grafts were examined 6-40 weeks posttransplantation. Host retinal input was assessed by injecting the host eyes with either [3H]proline, horseradish peroxidase (HRP) or wheat-germ agglutinin conjugated HRP. The host cortical projection was examined using anterograde degeneration techniques. Frozen tissue sections were also stained for Nissl, neurofibrils or reacted for acetylcholinesterase (AChE). All 3 DIV and 7 DIV explants survived transplantation and many grew considerably in size within the host brain. 14 DIV grafts were smaller and were found in only 50% of host brains. The cellular organization, fibre architecture and pattern of AChE staining in cultured grafts was similar to that found in non-cultured tectal transplants. Furthermore, host retina and cortex projected into the grafts in a manner similar to their innervation of non-cultured tectal tissue.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Anatomy, University of Western Australia, Nedlands.
authors.forename: A R
authors.initials: AR
authors.lastname: Harvey
authors.forename: R A
authors.initials: RA
authors.lastname: Rush
authors.forename: P J
authors.initials: PJ
authors.lastname: Keating
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology* / Q000502:physiology; D005333:Fetus; D006085:Graft Survival; D008636:Mesencephalon / Q000166:cytology / Q000502:physiology*; D051381:Rats; D012160:Retina / Q000166:cytology* / Q000502:physiology; D013477:Superior Colliculi / Q000166:cytology / Q000196:embryology / Q000637:transplantation*; D014795:Visual Pathways / Q000502:physiology*