title: Comparison of particle clearance and macrophage phagosomal motion in liver and lungs of rats.
abstract: Magnetic particles and magnetometry were used to noninvasively measure motion of particle-containing organelles in macrophages as well as to monitor the disappearance of particles from tissues. We compared these parameters in the liver (where macrophages are attached to the endothelium) and in the lungs (where macrophages were mobile on epithelial surfaces). Submicrometric magnetic particles were injected intravenously (1.5 mg/kg) into rats; 94% was taken up by the liver. Rats were also instilled intratracheally (1.0 mg/kg) with the same particles. Ultrastructural analyses showed that almost all particles were ingested by macrophages in both organs. Periodically, the retained particles were magnetized and aligned with an external magnet. After the magnet was removed, the decay of the resulting remanent field (relaxation) was followed for 25 min. Relaxation parameters (t1/2 and lambda 0) in the liver were constant from 30 min to 30 days after particle administration, but relaxation in lungs showed a time-dependent increase during the 1st day due to the slower rate of particle phagocytosis. Relaxation in both organs primarily reflects the motion of particle-containing organelles as they are rotated by the cytoskeleton. Relaxation in the lungs may also reflect cell translocation or even changes in alveolar shape. Clearance of particles from the lungs or liver was measured by following B0 (initial magnetic field strength). After correction for growth, the clearance t1/2 was 17.7 and 27.3 days for the lungs and liver, respectively. Bulk transport of particles is probably a more important clearance mechanism in the lungs than in the liver.
authors.affiliation: Department of Environmental Science and Physiology, Harvard University School of Public Health, Boston, Massachusetts 02115.
authors.forename: S B
authors.initials: SB
authors.lastname: Weinstock
authors.forename: J D
authors.initials: JD
authors.lastname: Brain
mesh_terms: D000818:Animals; D005290:Ferric Compounds; D007700:Kinetics; D007728:Kupffer Cells / Q000502:physiology; D008099:Liver / Q000502:physiology*; D008168:Lung / Q000502:physiology*; D008264:Macrophages / Q000502:physiology*; D008280:Magnetics; D008297:Male; D008854:Microscopy, Electron; D009068:Movement; D015388:Organelles / Q000502:physiology; D010587:Phagocytosis; D010588:Phagosomes / Q000502:physiology*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D005290:Ferric Compounds; C000499:ferric oxide