Use of 86Rb and 22Na in assaying active and cotransport activities in human erythrocytes in a biracial population.
A defect in Na+-K+ transport across the red cell membrane has been shown to be associated with essential hypertension. A sensitive assay system to measure active, co- and countertransport systems in erythrocytes from normotensive adults was developed. Active, co- and countertransport systems in the erythrocytes were assayed by measuring the influx of radioactive 22Na+ and 86Rb+. In the biracial (black-white) population group studied, analysis of variance of the active transport showed a significant race effect (p = 0.003). Cotransport activity showed age by race interaction (p = 0.001) and age by sex (p = 0.02). Cotransport activity was significantly higher in whites than blacks (p = 0.0001). Countertransport activity did not vary either by sex or race. Of the Spearman correlation coefficients for transport activities and blood pressure, white males showed a strong positive correlation with countertransport, whereas in black males, blood pressures showed a strong interaction with active transport. Among the transport activities, active transport showed significant interaction with countertransport activity in black males, whereas cotransport activity in whites showed a strong interaction with countertransport. The results suggest a subtle difference in Na+-K+ transport systems between blacks and whites, and these variations may be related to differences for susceptibility to essential hypertension.
Sharma
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C
Department of Medicine, Louisiana State University Medical Center, New Orleans 70112.
Dalferes
E R
ER
Freedman
D S
DS
Asamoah
A
A
Berenson
G S
GS
D000328:Adult; D044383:Black People; D001794:Blood Pressure; D004912:Erythrocytes / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D012016:Reference Values; D012413:Rubidium / Q000493:pharmacokinetics*; D012964:Sodium / Q000493:pharmacokinetics*; D044465:White People
D012964:Sodium; D012413:Rubidium