Conductimetry: a new tool for studying inhibition of elastolysis.
The conductimetric method was applied to the measurement of human leukocyte elastase activity, using insoluble elastin as a substrate. From conductance changes, initial rates of elastolysis were derived. A linear relationship of enzyme activity with enzyme concentration was demonstrated up to 400nM of enzyme, for three different substrates. In this concentration range, inhibition of elastolysis by eglin c was studied for different concentrations of eglin c. A 50% inhibitory concentrations of 0.13-0.15 microM of eglin c was derived from our results, corresponding to an inhibitor/enzyme ratio of about 0.5, indicating a strong inhibition, as previously demonstrated by authors using synthetic substrates.
Saulnier
J
J
Laboratoire de Biochimie Analytique, Université Claude Bernard, Villeurbanne, France.
Bostancioglu
K
K
Favre-Bonvin
G
G
Wallach
J
J
D000818:Animals; D002417:Cattle; D004553:Electric Conductivity; D006801:Humans; D007700:Kinetics; D007962:Leukocytes / Q000201:enzymology*; D010196:Pancreatic Elastase / Q000032:analysis* / Q000037:antagonists & inhibitors / Q000302:isolation & purification; D011506:Proteins / Q000494:pharmacology; D015843:Serpins
D011506:Proteins; D015843:Serpins; C028656:eglin proteinase inhibitors; D010196:Pancreatic Elastase