title: NADP-dependent isocitrate dehydrogenase from bass (Dicentrarchus labrax L.) liver.
abstract: The isocitrate dehydrogenase from bass liver was purified to homogeneity by gel filtration, affinity and ion exchange chromatographies. The molecular weight was estimated by gel filtration chromatography to about 120,000. Analysis of the enzyme on sodium dodecyl sulphate polyacrylamide gel electrophoresis showed it to be a dimeric protein. The enzyme showed maximum activity in the pH range between 7.0 and 8.0 while its maximum activity was at pH 7.5. DL-Isocitrate and Mn2+ stabilized the enzyme, while NADP had the opposite effect. The Km for isocitrate was 0.31 mM and the Km for NADP was 36 microM.
authors.affiliation: Departamento de Bioquímica, Facultad de Veterinaria, Universidad Complutense de Madrid, Spain.
authors.forename: M M
authors.initials: MM
authors.lastname: Medina-Puerta
authors.forename: M
authors.initials: M
authors.lastname: Gallego-Iniesta
authors.forename: A
authors.initials: A
authors.lastname: Garrido-Pertierra
mesh_terms: D000818:Animals; D001492:Bass / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007521:Isocitrate Dehydrogenase / Q000302:isolation & purification / Q000378:metabolism*; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008274:Magnesium / Q000494:pharmacology; D008345:Manganese / Q000494:pharmacology; D008970:Molecular Weight; D009249:NADP / Q000494:pharmacology; D010473:Perciformes / Q000378:metabolism*; D011487:Protein Conformation; D013696:Temperature
chemical_list: D008345:Manganese; D009249:NADP; D007521:Isocitrate Dehydrogenase; D008274:Magnesium