[Changes in the content of potassium, sodium and potassium influx in human peripheral blood lymphocytes during long-term cultivation with phytohemagglutinin].
Intracellular potassium, sodium and potassium influx were examined in PHA-activated human lymphocytes within 6 days of cultivation. DNA flow cytometry was used to estimate the percentage of cells in G1, S and G2 + M phases. Potassium influx and content per g protein were found to be increased, whereas sodium content decreased with the progression of cells from G1 to S phases, being maximum on the 3rd day. Later on the percentage of cells in S phase was seen diminished, and the potassium content decreased just as sodium content increased. It is concluded that ionic changes may correlate with the entering of cells into S phase.
Ezhevskiĭ
S A
SA
Vinogradov
A E
AE
Kazhdan
I Ia
IIa
Tsveĭbakh
A S
AS
D002453:Cell Cycle / Q000187:drug effects; D002478:Cells, Cultured; D005434:Flow Cytometry; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D010835:Phytohemagglutinins / Q000494:pharmacology*; D011188:Potassium / Q000097:blood*; D015221:Potassium Channels / Q000032:analysis / Q000378:metabolism*; D012964:Sodium / Q000097:blood*; D013997:Time Factors
D010835:Phytohemagglutinins; D015221:Potassium Channels; D012964:Sodium; D011188:Potassium
Izmenenie soderzhaniia kaliia, natriia i vkhodnykh potokov kaliia v limfotsitakh perifericheskoĭ krovi cheloveka pri dlitel'nom kul'tivirovaniia s fitogemaggliutininom.