Effect of n-butyrate on the synthesis of sulfated glycosaminoglycans and hyaluronate by rat liver fat-storing cells (Ito cells).
The effect of naturally occurring aliphatic carboxylic acid n-butyrate on total and type-specific synthesis of sulfated proteoglycans and hyaluronic acid by rat liver fat-storing cells (Ito cells, vitamin A-storing cells), the main connective tissue producing cell type in liver, was studied. Concentrations of n-butyrate equal to and higher than 5 mM inhibited significantly in a dose-dependent manner the incorporation of [35S]sulfate and [3H]glucosamine into the carbohydrate chains of sulfated proteoglycans. Maximum inhibition of 70% was reached at 15 mM. Similarly, the formation of hyaluronic acid was impaired by n-butyrate. The synthesis profile of specific sulfated proteoglycans was not affected by the compound. beta-Xyloside, an artificial carbohydrate chain initiator, reversed only partially the inhibitory effect of n-butyrate. Thus, the mechanism of n-butyrate inhibition may involve an impairment of both proteoglycan core protein formation and glycosaminoglycan chain elongation.
Gressner
A M
AM
Department of Clinical Chemistry and Central Laboratory, Philipps-University, Marburg, Federal Republic of Germany.
Haarmann
R
R
D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D002087:Butyrates / Q000494:pharmacology*; D020148:Butyric Acid; D006025:Glycosaminoglycans / Q000096:biosynthesis*; D006820:Hyaluronic Acid / Q000096:biosynthesis*; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism*; D008297:Male; D011509:Proteoglycans / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013431:Sulfates / Q000378:metabolism*
D002087:Butyrates; D006025:Glycosaminoglycans; D011509:Proteoglycans; D013431:Sulfates; D020148:Butyric Acid; D006820:Hyaluronic Acid