Effects of a cysteine precursor, L-2-oxothiazolidine-carboxylate, nutritional status, and sex on tissue glutathione and hepatic GSH-utilizing enzymes of CD-1 mice.
Objectives of this study were to compare the effects of sex, nutritional status and L-2-oxothiazolidine carboxylate (OTC) treatment on tissue constituents frequently involved in responses to chemical toxins. Four groups of adult CD-1 mice were studied: fed females, fed males, fasted males, and fasted males three hours after treatment with OTC (10 mmoles/kg, sc). Female fed mice were found to differ from male fed mice as follows: lower tissue GSH in liver and kidney but not lung; lower hepatic microsomal cytochrome P-450 content and cytosolic GSH transferase activities, particularly using CDNB as substrate; and higher hepatic GSH peroxidase but similar GSSG reductase activities. Overnight fasting was associated with a decrease in hepatic and renal GSH and hepatic cytochrome P-450. OTC treatment was only found to increase hepatic GSH and decrease renal GSH. Thus in fasted CD-1 male mice, the intracellular cysteine precursor, OTC, has an apparently selective effect on tissue GSH contents without confounding effects on hepatic GSH utilizing or restoring activities.
Moslen
M T
MT
Department of Pathology, University of Texas Medical Branch, Galveston 77550.
Harper
B L
BL
Roy
D
D
D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000032:analysis; D005260:Female; D005978:Glutathione / Q000032:analysis* / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000032:analysis; D005980:Glutathione Reductase / Q000032:analysis; D005982:Glutathione Transferase / Q000032:analysis; D008099:Liver / Q000201:enzymology*; D008297:Male; D051379:Mice; D009752:Nutritional Status; D011761:Pyrrolidonecarboxylic Acid; D012737:Sex Factors; D013844:Thiazoles / Q000494:pharmacology*; D053778:Thiazolidines
D013844:Thiazoles; D053778:Thiazolidines; D003577:Cytochrome P-450 Enzyme System; D005979:Glutathione Peroxidase; D005980:Glutathione Reductase; D005982:Glutathione Transferase; D005978:Glutathione; D011761:Pyrrolidonecarboxylic Acid; C029536:2-oxothiazolidine-4-carboxylic acid