title: Growth requirements, growth factor responsiveness, and growth factor secretion of three human embryonal carcinoma cell lines.
abstract: The in vitro growth requirements of three human embryonal carcinoma cell lines (H 12.7, 2102 EP, 1428 A) were investigated. The basal medium DME/F12 supplemented with insulin, transferrin, and low-density and high-density lipoproteins was sufficient to support substantial multiplication of all three lines. The most efficient attachment factor was either fibronectin (for 2102 EP and 1428 A) or collagen type I (H 12.7). In a serum-free system the influence of epidermal growth factor (EGF), insulin-like growth factor I, multiplication stimulating activity (MSA), a platelet extract, and the glucocorticoids dexamethasone and hydrocortisone, as determined by the DNA synthesis rate of the cells, was generally minimal. However, the DNA synthesis rate of cell lines H 12.7 and 2102 EP was increased by MSA, and the line with the highest potential to differentiate (H 12.7) was stimulated by EGF. All three cell lines secreted growth factors in a heterologous stimulation assay. Insulin-like growth factors I and II were not part of the growth promoting activity. The inhibitory effect of a monoclonal anti-EGF antibody on the 3H-thymidine incorporation of cell line 2102 EP might indicate autocrine secretion of EGF or an EGF-like factor by this cell line.
authors.affiliation: Medizinische Hochschule Hannover, Abteilung Hämatologie-Onkologie, Federal Republic of Germany.
authors.forename: W
authors.initials: W
authors.lastname: Verbeek
authors.forename: C
authors.initials: C
authors.lastname: Bokemeyer
authors.forename: H
authors.initials: H
authors.lastname: Falk
authors.forename: H J
authors.initials: HJ
authors.lastname: Schmoll
mesh_terms: D002455:Cell Division; D003470:Culture Media; D004247:DNA / Q000096:biosynthesis; D054278:Embryonal Carcinoma Stem Cells; D066246:ErbB Receptors / Q000032:analysis; D006133:Growth Substances / Q000378:metabolism* / Q000494:pharmacology; D006801:Humans; D014411:Neoplastic Stem Cells / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D014407:Tumor Cells, Cultured
chemical_list: D003470:Culture Media; D006133:Growth Substances; D004247:DNA; D066246:ErbB Receptors