A two-dimensional high-performance thin-layer chromatographic screening method for sulphonamides in animal tissues.
A procedure for the identification of sulphonamides in edible animal tissues by two-dimensional high-performance thin-layer chromatography is described. Sixteen sulphonamides can be detected at an absolute level of 10 ng. The absolute detection limit of the sulphonamide standards is 1 ng. After extraction of the sulphonamides with chloroform/acetone, the acidified extract is concentrated and purified using a cation-exchange solid phase extraction column. The column is treated with ammonia vapour and the sulphonamides are then eluted with methanol. Blank samples of edible animal tissues spiked with sulphonamides (frequently used in Belgium) result in very good separation.
Wyhowski de Bukanski
B
B
Ministère de la Santé Publique et de l'Environnement, Brussels, Belgium.
Degroodt
J M
JM
Beernaert
H
H
D000818:Animals; D002855:Chromatography, Thin Layer; D004350:Drug Residues / Q000032:analysis*; D008460:Meat / Q000032:analysis*; D008461:Meat Products / Q000032:analysis*; D013449:Sulfonamides / Q000032:analysis*
D013449:Sulfonamides