title: Cell cycle dependence of inositol phosphate levels in neuroblastoma cells.
abstract: To investigate the timing of inositol lipid turnover in relation to the cell cycle, inositol phosphates and lipids were measured in neuroblastoma (Neuro-2A) cells that were prelabeled with [3H]inositol and synchronized by a mitotic shakeoff technique. Distinct early and late phases of inositol phosphate production were identified. The early peak occurs between the 2nd and 4th hour after mitosis near the G1/S transition. A later peak occurs around the peak of S phase (DNA synthesis) at 7-8 h after mitosis. These findings suggest that activation of phosphatidylinositol turnover generates signals that play a role in cell cycle progression.
authors.affiliation: Department of Physiology, Tufts University School of Medicine, Boston, Massachusetts 02111.
authors.forename: L F
authors.initials: LF
authors.lastname: Fleischman
authors.forename: L
authors.initials: L
authors.lastname: Cantley
mesh_terms: D000818:Animals; D002453:Cell Cycle; D002460:Cell Line; D004261:DNA Replication; D007294:Inositol / Q000378:metabolism; D007295:Inositol Phosphates / Q000378:metabolism*; D007700:Kinetics; D009447:Neuroblastoma / Q000473:pathology*; D013403:Sugar Phosphates / Q000378:metabolism*; D013936:Thymidine / Q000378:metabolism
chemical_list: D007295:Inositol Phosphates; D013403:Sugar Phosphates; D007294:Inositol; D013936:Thymidine