title: Evaluation of an enzyme-linked immunosorbent assay to actin in cultured fibroblasts.
abstract: The amount of actin in cultured human skin fibroblasts was determined by an enzyme-linked immunosorbent assay. Absorbance values (OD), resulting from specific binding of an antiactin monoclonal antibody to intracellular actin, were converted to cell concentrations (pg/cell) from an actin standard curve. Actin concentration for cultured human skin fibroblasts by this technique was estimated to be 55.5 picograms/cell and constituted approximately 3.9% of the total cellular protein. This assay procedure offers the following advantages: (1) It is time efficient, can be completed in 2-3 hours; (2) cells are unaltered except for membrane permeabilization; (3) the sensitivity is equal to or greater than previous procedures involving gel electrophoresis; and (4) the assay is easy and inexpensive to perform.
authors.affiliation: San Diego Veterans Administration Medical Center, CORE Electron Microscopy Unit, San Diego, Ca. 92161.
authors.forename: J S
authors.initials: JS
authors.lastname: Vande Berg
authors.forename: D R
authors.initials: DR
authors.lastname: Disharoon
authors.forename: W L
authors.initials: WL
authors.lastname: Poolman
authors.forename: R
authors.initials: R
authors.lastname: Rudolph
mesh_terms: D000199:Actins / Q000032:analysis* / Q000592:standards; D002452:Cell Count; D002469:Cell Separation; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D005069:Evaluation Studies as Topic; D005347:Fibroblasts / Q000032:analysis*; D006801:Humans; D012015:Reference Standards
chemical_list: D000199:Actins