Quantitative analysis of the distribution of serotonin-immunoreactive cell bodies in the mouse brain.
The distribution of serotonin neurons in the mouse brain was studied using a peroxidase-antiperoxidase (PAP) immunohistochemical method to obtain quantitative data on serotonin-containing cell bodies. The average total number of perikarya of serotonin neurons was about 26,000, of which about 17,000 cells (66.9%) were situated in the raphe system. The nucleus raphe dorsalis was the main region where a large number of serotonin neurons were present, and it contained about 9000 cells. Other than the raphe system, serotonin-immunoreactive perikarya were seen in the following regions: the reticular formation (18.9%), the lemniscus medialis (8.5%), the nucleus interpeduncularis (1.5%), the subpyramidal region (4.0%) and the periaqueductal gray (0.2%).
Ishimura
K
K
Department of Pediatrics, Kyoto Prefectural University of Medicine, Japan.
Takeuchi
Y
Y
Fujiwara
K
K
Tominaga
M
M
Yoshioka
H
H
Sawada
T
T
D000818:Animals; D001921:Brain / Q000166:cytology* / Q000378:metabolism; D002452:Cell Count; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009474:Neurons / Q000145:classification / Q000378:metabolism*; D012701:Serotonin / Q000378:metabolism*
D012701:Serotonin