title: [The effect of microenvironment and formation of intra-molecular links on the tertiary structure of cytochrome P-450 LM2].
abstract: The effect of intramolecular cross-links formation in isolated cytochrome P-450 LM2 on its reactivation after incorporation into the liposome lipid bilayer was studied. Treatment with bifunctional reagents results in the inactivation of the solubilized haemoprotein. The degree of the enzyme immobilization determines the degree of inhibition of p-nitroanisol demethylation and aniline hydroxylation. Whereas the complete inhibition of oxidation of type II substrate turnover needs two intramolecular cross-links, that of type I substrates necessitates at least seven cross-links. The incorporation of modified and native enzymes into the membrane lipid bilayer at temperatures above the phase transition point results in the enzyme activation. However, in case of the preimmobilized enzyme the activation does not reach the maximal values. Both stabilized and liposome-incorporated cytochrome P-450 can fully be reactivated via the cross-link disulfide bond reduction. No such effect is observed at temperatures below the phase transition point.
authors.forename: V Iu
authors.initials: VIu
authors.lastname: Uvarov
authors.forename: A V
authors.initials: AV
authors.lastname: Leshchenko
authors.forename: I G
authors.initials: IG
authors.lastname: Rukavishnikov
authors.forename: Ch S
authors.initials: ChS
authors.lastname: Dzhuzenova
authors.forename: A I
authors.initials: AI
authors.lastname: Archakov
mesh_terms: D002384:Catalysis; D003432:Cross-Linking Reagents; D065607:Cytochrome P-450 Enzyme Inhibitors; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D004789:Enzyme Activation; D007700:Kinetics; D008051:Lipid Bilayers; D010084:Oxidation-Reduction; D011487:Protein Conformation; D012995:Solubility; D013379:Substrate Specificity
chemical_list: D003432:Cross-Linking Reagents; D065607:Cytochrome P-450 Enzyme Inhibitors; D008051:Lipid Bilayers; D003577:Cytochrome P-450 Enzyme System
vernacular_title: Vliianie mikrookruzheniia i obrazovaniia vnutrimolekuliarnykh shivok na formirovanie tretichnoĭ struktury tsitokhroma LM2.