NH4+ ion-selective microelectrode based on the antibiotics nonactin/monactin.
A liquid-membrane microelectrode (less than or equal to 1 micron tip diameter) using macrotetrolide antibiotics as ion-selective components is described. The electrode shows selectivities of NH4+ over K+, Na+ and H+ of 3.8, 100 and 150, respectively. The stability and reproducibility of the sensor signal and the response time are determined in solutions with a typical intracellular ion background. The microelectrode does not suffer from significant interference by inorganic and organic inhibitors and lipophilic cations, but high concentrations of lipophilic anions may interfere considerably.
Bührer
T
T
Department of Organic Chemistry, Swiss Federal Institute of Technology (ETH), Zürich.
Peter
H
H
Simon
W
W
D000900:Anti-Bacterial Agents; D004594:Electrophysiology / Q000295:instrumentation*; D066298:In Vitro Techniques; D018942:Macrolides; D008839:Microelectrodes; D000644:Quaternary Ammonium Compounds
D000900:Anti-Bacterial Agents; D018942:Macrolides; D000644:Quaternary Ammonium Compounds; C100287:monactin; C050476:nonactin