title: Changes in protein synthetic activity in early Drosophila embryos mutant for the segmentation gene Krüppel.
abstract: We have identified early embryo proteins related to the segmentation gene Krüppel by [35S]methionine pulse labelling and two-dimensional gel electrophoresis. Protein synthesis differences shared by homozygous embryos of two Krüppel alleles when compared to heterozygous and wild-type embryos are reported. The study was extended to syncytial blastoderm stages by pulse labelling and gel analysis of single embryos, using Krüppel-specific proteins from gastrula stages as molecular markers for identifying homozygous Krüppel embryos. Localized expression of interesting proteins was examined in embryo fragments. The earliest differences detected at nuclear migration stages showed unregulated synthesis in mutant embryos of two proteins that have stage specific synthesis in normal embryos. At the cellular blastoderm stage one protein was not synthesized and two proteins showed apparent shifts in isoelectric point in mutant embryos. Differences observed in older embryos included additional proteins with shifted isoelectric points and a number of qualitative and quantitative changes in protein synthesis. Five of the proteins with altered rates of synthesis in mutant embryos showed localized synthesis in normal embryos. The early effects observed are consistent with the hypothesis that the Krüppel product can be a negative or positive regulator of expression of other loci, while blastoderm and gastrula stage shifts in isoelectric point indicate that a secondary effect of Krüppel function may involve post-translational modification of proteins.
authors.affiliation: Department of Biology, Clarkson University, Potsdam, NY 13676.
authors.forename: V
authors.initials: V
authors.lastname: Bedian
authors.forename: M C
authors.initials: MC
authors.lastname: Summers
authors.forename: S A
authors.initials: SA
authors.lastname: Kauffman
mesh_terms: D000483:Alleles; D000818:Animals; D001756:Blastoderm / Q000378:metabolism; D004330:Drosophila / Q000196:embryology* / Q000235:genetics / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D005775:Gastrula / Q000378:metabolism; D006579:Heterozygote; D006720:Homozygote; D009154:Mutation; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics; D013462:Sulfur Radioisotopes
chemical_list: D011506:Proteins; D013462:Sulfur Radioisotopes