Hemodialysis with calcium-free dialysate prevents myocardial creatine kinase depletion after brief coronary artery occlusion in dogs.
To evaluate the effect of hypocalcemia on myocardial creatine kinase (CK) depletion after brief coronary artery occlusion and reperfusion, dogs were rendered hypocalcemic via systemic hemodialysis for eighty minutes in the absence of Ca. Control animals were hemodialysed in the presence of Ca. The left anterior descending coronary artery was then occluded for six minutes and reperfusion for eighty minutes occurred at low flow of dialysate. A 50% decrease in serum Ca of the hypocalcemic animals during the eighty minutes of hemodialysis resulted in a significant (about 35%) decrease of myocardial Ca. Comparison of the myocardial creatine kinase activity following reperfusion showed preservation of the enzyme in the ischemic areas of the hypocalcemic animals, whereas the CK activities of the ischemic areas of the normocalcemic animals were much lower (p less than 0.005). During the reperfusion period serum Ca of the hypocalcemic group increased to 75% of that of the normocalcemic group while myocardial Ca of both ischemic and nonischemic areas reequilibrated to normocalcemic values. Hemodynamic parameters during the various phases of the experiment were not altered significantly. It is concluded that transient decrease of myocardial Ca produced by hypocalcemia prior to occlusion leads to protection against myocardial damage after brief coronary ligation.
Darsinos
J
J
Department of Medicine, University of Athens, Greece.
Karli
J
J
Pistevos
A
A
Levis
G
G
Moulopoulos
S
S
D000818:Animals; D002118:Calcium / Q000097:blood / Q000378:metabolism*; D003250:Constriction; D003331:Coronary Vessels; D003402:Creatine Kinase / Q000378:metabolism*; D004285:Dogs; D007527:Isoenzymes; D015425:Myocardial Reperfusion; D009206:Myocardium / Q000201:enzymology*; D006435:Renal Dialysis
D007527:Isoenzymes; D003402:Creatine Kinase; D002118:Calcium