title: Analysis of allergenic components of a Parietaria judaica pollen extract by chromatographic methods for the evaluation of purification procedures.
abstract: Size-exclusion and ion-exchange high-performance liquid chromatography were used to monitor the presence of polymeric aggregates and of families of allergens of similar isoelectric point in Parietaria judaica pollen extracts. A radio-allergo-sorbent test and chromatofocusing were used for the analysis of individual fractions. This allows the detection of two groups of allergens, having pI of 4.7 and greater than 5.7, respectively, and shows the effect of dialysis or ultrafiltration on the distribution of allergens.
authors.affiliation: Dipartimento di Chimica Organica e Industriale, Universita' di Milano, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Bassoli
authors.forename: F
authors.initials: F
authors.lastname: Chioccara
authors.forename: G
authors.initials: G
authors.lastname: Di Gregorio
authors.forename: B
authors.initials: B
authors.lastname: Rindone
authors.forename: S
authors.initials: S
authors.lastname: Tollari
authors.forename: P
authors.initials: P
authors.lastname: Falagiani
authors.forename: G
authors.initials: G
authors.lastname: Riva
authors.forename: E
authors.initials: E
authors.lastname: Bolzacchini
mesh_terms: D000485:Allergens / Q000032:analysis*; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D005069:Evaluation Studies as Topic; D007525:Isoelectric Focusing; D010936:Plant Extracts / Q000032:analysis*; D011058:Pollen / Q000032:analysis*; D011852:Radioallergosorbent Test
chemical_list: D000485:Allergens; D010936:Plant Extracts