"In vivo" (35S)-methionine interaction with rat-liver DNA and the effect of chemical carcinogens.
1. After intraperitoneal administration of (35S)methionine (25 mg, 1.6 mCi/kg), detectable amount of radioactivity resulted associated to rat-liver DNA: the interaction reached the maximum value (about 18 pmol/mumol DNA P) by 2 h after administration of radioactive aminoacid. 2. The (35S)-binding was inhibited by the hepatocarcinogenic ethionine and dimethylnitrosamine, and was stimulated by the non-hepatocarcinogenic methylnitrosourea. 3. Hplc analysis of (35S)DNA enzymic digest evidenced two radioactive compounds, the UV behaviour of which is reported.
Kanduc
D
D
Dipt. di Biochimica e Biologia Molecolare, Facoltà di Scienze, Università di Bari, Italy.
Quagliariello
E
E
D000818:Animals; D002273:Carcinogens / Q000494:pharmacology; D004247:DNA / Q000378:metabolism*; D004249:DNA Damage; D008099:Liver / Q000378:metabolism; D008297:Male; D008715:Methionine / Q000378:metabolism* / Q000493:pharmacokinetics; D051381:Rats; D011919:Rats, Inbred Strains; D013056:Spectrophotometry, Ultraviolet
D002273:Carcinogens; D004247:DNA; D008715:Methionine