Proteoglycan biosynthesis by rabbit articular chondrocytes treated with D-penicillamine.
Rabbit articular chondrocytes in confluent monolayer cultures were treated with D-Penicillamine (D-Pen) during 3 or 5 days. The [35S]-sulfate incorporation in neosynthesized proteoglycans was not modified by D-Pen doses ranging from 50 to 800 micrograms/ml. After treatment during 5 days with D-Pen concentrations of 50 or 400 micrograms/ml, the chemical characteristics of proteoglycans from medium and cell-layer were determined. The aggregation capacity of proteoglycans from medium, the monomer molecular size, the glycosaminoglycan chain length and the relative rates of the different glycosaminoglycans (chondroitins, chondroitin 6-sulfate, chondroitin 4-sulfate, hyaluronic acid) remained unchanged. These results suggest that D-Pen does not alter some of the cartilage mechanical properties due to the presence of proteoglycans.
Legendre
P
P
Laboratoire de Biochimie du Tissu Conjonctif, U.F.R. de Sciences, Caen, France.
Bouakka
M
M
Langris
M
M
Pujol
J P
JP
Beliard
R
R
Loyau
G
G
Bocquet
J
J
D000818:Animals; D002358:Cartilage, Articular / Q000187:drug effects* / Q000378:metabolism; D002478:Cells, Cultured; D002850:Chromatography, Gel; D005109:Extracellular Matrix / Q000378:metabolism; D006025:Glycosaminoglycans / Q000096:biosynthesis / Q000302:isolation & purification; D008968:Molecular Conformation; D008970:Molecular Weight; D010396:Penicillamine / Q000494:pharmacology*; D011509:Proteoglycans / Q000096:biosynthesis* / Q000302:isolation & purification; D011817:Rabbits
D006025:Glycosaminoglycans; D011509:Proteoglycans; D010396:Penicillamine