Quality control of hemoglobin-based blood substitutes.
Preparation and use of hemoglobin-based blood substitutes, from stroma-free hemoglobin (SFH or Hb) and its DPG analogue-modified derivatives (PLP-Hb, ATP-Hb etc.) without thorough characterization and quality control in animal or human testing have produced, and may continue to produce, artifacts in the finished product. Thus the development of such a natural substitute for the volume expansion and oxygen delivery functions of the blood will be impeded. A case is made for the use of affinity purified hemoglobin and modified hemoglobin as standard starting materials for the preparation of Hb-based blood substitute(s) in general, and in particular poly PLP-Hb. Development of a commercial scale blood-substitute is only possible after the safety and toxicity issues of substitutes have been resolved by applying rigorous quality control.
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Biosciences Division, Defence and Civil Institute of Environmental Medicine, Downsview, Ontario, Canada.
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D000255:Adenosine Triphosphate / Q000031:analogs & derivatives / Q000302:isolation & purification; D001802:Blood Substitutes / Q000302:isolation & purification* / Q000592:standards; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D004163:Diphosphoglyceric Acids; D006454:Hemoglobins / Q000302:isolation & purification* / Q000592:standards; D010952:Plasma Substitutes; D011732:Pyridoxal Phosphate / Q000031:analogs & derivatives / Q000302:isolation & purification; D011786:Quality Control; D012996:Solutions; D014462:Ultrafiltration
D001802:Blood Substitutes; D004163:Diphosphoglyceric Acids; D006454:Hemoglobins; D010952:Plasma Substitutes; D012996:Solutions; C051530:adenosine triphosphate-stroma-free hemoglobin; C016484:hemoglobin, stroma free; C030939:polyhemoglobin-pyridoxal-5-phosphate; D011732:Pyridoxal Phosphate; D000255:Adenosine Triphosphate