[Histochemical and ultrastructural studies on anti-edematous and radiation-protective action of 0-(beta-hydroxyethyl)-rutosides on the rat brain after single irradiation. 2. Histochemical study on carbohydrate metabolism and acid phosphatase activation].
The disturbances of the carbohydrate metabolism and the activation of acid phosphatases with and without protection by O-(beta-hydroxyethyl)-rutoside (HR) were studied in irradiated rat brains by means of the light microscope. The histochemically demonstrable deposition of glycogen and acid mucopolysaccharides serves as a criterion for a reversible lesion of the irradiated cerebral tissue. The extent of local activation of repair processes following to irradiation can be determined by the quantity of acid phosphatases in the lysosomes. For the dose range of 1 to 5 Gy, HR seems to exert a protective effect on the cellular metabolism of the irradiated cerebral tissue shown by a slight decrease of glycogen and mucopolysaccharide deposits compared to the untreated animals. However, when exceeding a threshold dose of 10 Gy, the deposition of carbohydrates in the HR group is strongly increased. A slightly increased activity of acid phosphatases induced by HR can be supposed at best for the dose range of 5 to 7.5 Gy. If the dose is even more increased, the reverse effect found in the carbohydrate metabolism, too, and an increased activation of acid phosphatases in the control group will be observed. This unexpected reversion of the protective effect could be related to the assumed inhibitory effect of HR on the ATPases and thus on the anaerobic part of glycolysis or to a breakdown of the cell interaction system of endothelial cells, glia cells, and neurons.
Thiel
H J
HJ
Strahlentherapeutische Klinik und Poliklinik der Universität Erlangen-Nürnberg.
Lierse
W
W
Lehmann
F M
FM
Sauer
R
R
D000135:Acid Phosphatase / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects / Q000648:ultrastructure; D001929:Brain Edema / Q000209:etiology / Q000517:prevention & control*; D050260:Carbohydrate Metabolism; D004789:Enzyme Activation; D006651:Histocytochemistry; D006896:Hydroxyethylrutoside / Q000494:pharmacology*; D011829:Radiation Dosage; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D051381:Rats; D012431:Rutin / Q000031:analogs & derivatives*
D006896:Hydroxyethylrutoside; D011837:Radiation-Protective Agents; D012431:Rutin; D000135:Acid Phosphatase
Histochemische und ultrastrukturelle Untersuchungen zur antiödematösen und radioprotektiven Wirkung von 0-(beta-Hydroxyäthyl)-rutosiden am Rattengehirn nach Einzeitbestrahlung. Teil 2. Histochemische Untersuchung des Kohlenhydratstoffwechsels und der Aktivierung saurer Phosphatasen.