Swelling tendency of rabbit cornea in organ culture medium. Influence of button size.
The swelling tendency of fresh rabbit corneas kept in organ culture was determined by measuring percentage weight increase. Corneal buttons with diameters of 5, 9, 14, and 16 mm had an average weight increase during the first hour of 53%, 35%, 18%, and 8.6%, respectively. Those with diameter 5 and 9 mm had a continuous weight increase up to a maximum of 350% to 450% of start weight after 25 h and 57 h, respectively. Many corneas with 16 mm diameter (including a thin scleral rim) and some with 14 mm, had a temporary stable weight of average 56% (with an individual cornea stabilizing at only 15%) above start weight between day 2 to day 7. Thereafter a phase with approximately linear weight increase ended at a maximum weight of 350% to 450% of start weight after one month. It is concluded that the rate of corneal swelling in cultivation is highly dependent upon the corneal button size.
Medin
W
W
University Eye Department, Rikshospitalet, University of Oslo, Norway.
Davanger
M
M
D000818:Animals; D001834:Body Water / Q000378:metabolism; D002463:Cell Membrane Permeability; D003315:Cornea / Q000033:anatomy & histology* / Q000378:metabolism; D009924:Organ Culture Techniques; D009929:Organ Size; D011817:Rabbits; D013997:Time Factors