Analysis of mutant frequency curves and survival curves applied to the AL hybrid cell system.
A model is presented for the statistical analysis of survival curves and mutant frequency curves for a hybrid cell system. The derivation of the model is given in the Appendix, and depends on simple assumptions about the distribution of insults, their repair, and the loss of a marker that is not rescued. A single formula (5) is found which relates a survival curve to the mutant frequency curve, i. e., the response curve for production of mutants per 10(5) survivors induced by a mutagen. The analysis is applied to loss of the a1 gene in AL-J1 hybrid cells submitted to Cesium gamma-rays. Previous experimental data using X-rays was reported by Waldren et al. (1986: Proc. Natl. Acad. Sci, USA 83, 4839.) Also, a derived formula (10), which predicts the probability that in a surviving cell a marker is lost and not rescued, will form the basis for testing the validity of the model in the future using new experimental data.
Parker
R
R
Johns Hopkins Medical Institutions, Baltimore, MD 21205.
Waldren
C
C
Hei
T K
TK
Wong
D F
DF
Puck
T T
TT
Puck
T L
TL
D000704:Analysis of Variance; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays; D006822:Hybrid Cells / Q000528:radiation effects*; D008957:Models, Genetic; D009154:Mutation; D011336:Probability