Changes in ribosomal properties during adenylate deprivation in the cells of Kluyveromyces lactis.
In an adenine-requiring mutant strain of the yeast, Kluyveromyces lactis, the intracellular content of ATP is one-third to one-fifth that in a prototrophic wild strain under growing conditions. The quantitative differences becomes rather small in resting stationary-phase cells. Temporary changes in the two-dimensional protein patterns of mutant ribosomes occur when the ATP content is lowest during the transition phase of growth. The transfer of exponentially growing cells to a synthetic complete medium void of adenine induces the same changes in mutant ribosomes within several hours. Identification of ribosomal proteins by two-dimensional gel electrophoresis indicated all changeable proteins (at least five proteins) to belong to 40S ribosomal subunits. The mutant ribosomes prepared from the transition-phase cells have much lower activity (below 60%) for poly(U)-directed polyphenylalanine synthesis than those in exponentially growing or resting stationary-phase cells. Thus, changes in ribosomal components associated with the differences in ribosome activity in a cell-free system were noted in the adenylate-deprived cells of K. lactis.
Ishiguro
J
J
Department of Biology, Faculty of Science, Konan University, Kobe, Japan.
Azuma
Y
Y
Uritani
M
M
Miyazaki
M
M
D000225:Adenine / Q000378:metabolism*; D000227:Adenine Nucleotides / Q000378:metabolism*; D000244:Adenosine Diphosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000032:analysis; D007716:Kluyveromyces / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D009154:Mutation; D012269:Ribosomal Proteins / Q000032:analysis; D012270:Ribosomes / Q000378:metabolism*; D004718:Saccharomycetales / Q000378:metabolism*
D000227:Adenine Nucleotides; D005656:Fungal Proteins; D012269:Ribosomal Proteins; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D000225:Adenine