Food intake suppression in rats by a substance isolated from human feces.
A substance which suppresses food intake was isolated from human feces. This substance was extracted and partially purified using Sephacryl S-200 column chromatography and a DEAE-Sephadex A-25 column chromatography. To assess the biological activity, rats were subjected to various applications. The intraperitoneal administration and the intracerebroventricular microinfusion of this substance suppresses short- and long-term food intake. The central infusion suppresses feeding with a potency over 1000 times that the peripheral administration. The central infusion of the heat-treated substance was without effect. These results suggest that a substance present in human feces (probably of a proteic nature) suppresses food intake in rats, at the level of the central nervous system.
Plata-Salamán
C R
CR
Department of Physiology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Yamamoto
T
T
Oomura
Y
Y
Shimizu
N
N
Sakata
T
T
D000818:Animals; D001067:Appetite Depressants / Q000302:isolation & purification*; D004435:Eating; D005215:Fasting; D005243:Feces / Q000032:analysis*; D006801:Humans; D007026:Hypothalamic Area, Lateral / Q000502:physiology; D008297:Male; D009842:Oligopeptides / Q000302:isolation & purification*; D011761:Pyrrolidonecarboxylic Acid / Q000031:analogs & derivatives; D051381:Rats; D014697:Ventromedial Hypothalamic Nucleus / Q000502:physiology
D001067:Appetite Depressants; D009842:Oligopeptides; C023866:pyroglutamyl-histidyl-glycine; D011761:Pyrrolidonecarboxylic Acid