title: [Cloning of human erythropoietin gene].
abstract: The 17-mer oligonucleotide probe homologous to the fragment of the gene for human erythrocyte differentiation factor erythropoietin was used to screen the human genomic library for this gene. Restriction analysis and partial sequencing of one of the identified clones have confirmed that the clone does contain the human erythropoietin gene. We are planning to use the cloned human erythropoietin gene for developing a stably transfected mammalian cell line that should secrete erythropoietin.
authors.forename: I A
authors.initials: IA
authors.lastname: Kramerova
authors.forename: N S
authors.initials: NS
authors.lastname: Neznanov
authors.forename: V M
authors.initials: VM
authors.lastname: Bozhkov
authors.forename: A A
authors.initials: AA
authors.lastname: Arzumanov
authors.forename: K G
authors.initials: KG
authors.lastname: Gazarian
mesh_terms: D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D015342:DNA Probes; D004921:Erythropoietin / Q000235:genetics*; D006801:Humans; D009693:Nucleic Acid Hybridization; D015183:Restriction Mapping
chemical_list: D015342:DNA Probes; D004921:Erythropoietin; D004247:DNA
vernacular_title: Klonirovanie gena éritropoétina cheloveka.