A gene-cloning system for Kluyveromyces lactis and isolation of a chromosomal gene required for killer toxin production.
A transformation system derived from the circular plasmid pKD1 has been developed for Kluyveromyces lactis. The principle is essentially equivalent to that of the 2 microns/Saccharomyces cerevisiae transformation system. The main features of the system are presented. Using a pKD1-based DNA bank of K. lactis, the KEX1 gene involved in the killer system was isolated by complementation.
Chen
X J
XJ
Institut Curie, Centre Universitaire Paris-Sud, Orsay, France.
Wésolowski-Louvel
M
M
Tanguy-Rougeau
C
C
Bianchi
M M
MM
Fabiani
L
L
Saliola
M
M
Falcone
C
C
Frontali
L
L
Fukuhara
H
H
D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000235:genetics; D005656:Fungal Proteins / Q000096:biosynthesis / Q000235:genetics*; D005800:Genes, Fungal; D005822:Genetic Vectors; D055646:Killer Factors, Yeast; D007716:Kluyveromyces / Q000235:genetics* / Q000378:metabolism; D009183:Mycotoxins / Q000096:biosynthesis / Q000235:genetics*; D010957:Plasmids; D011401:Promoter Regions, Genetic; D029701:Saccharomyces cerevisiae Proteins; D004718:Saccharomycetales / Q000235:genetics*; D014170:Transformation, Genetic
D004271:DNA, Fungal; D005656:Fungal Proteins; C527157:KHR1 protein, S cerevisiae; D055646:Killer Factors, Yeast; D009183:Mycotoxins; D029701:Saccharomyces cerevisiae Proteins