Cloning and chromosomal location of human genes inducible by type I interferon.
When cells are treated with interferon several new proteins are induced. We have isolated by differential screening two cDNA clones corresponding to human genes inducible by IFN-alpha, termed IFI-4 and IFI-54K. The accumulation of the corresponding mRNA was followed as a function of either IFN dose or of time. The IFI-4 and IFI-54K genes, as well as two previously isolated IFN-inducible genes, namely the IFI-56K and low-molecular-weight 2-5A synthetase, were localized on the human chromosomes. Using cloned probes on Southern blots of DNA from a panel of rodent-human somatic cell hybrids, we have assigned the IFI-4 gene to chromosome 1 and the gene coding for the low-molecular-weight 2-5A synthetase to chromosome 12. We also showed that the IFI-54K and IFI-56K genes, unlike most of the IFN-inducible genes, are syntenic. They are both located on chromosome 10. In addition, evidence is given for the presence of a pseudogene homologous to IFI-56K on chromosome 13.
Wathelet
M G
MG
Département de Biologie Moléculaire, Université Libre de Bruxelles, Belgium.
Szpirer
J
J
Nols
C B
CB
Clauss
I M
IM
De Wit
L
L
Islam
M Q
MQ
Levan
G
G
Horisberger
M A
MA
Content
J
J
Szpirer
C
C
D015088:2',5'-Oligoadenylate Synthetase / Q000235:genetics*; D000818:Animals; D015152:Blotting, Northern; D015139:Blotting, Southern; D002460:Cell Line; D002874:Chromosome Mapping; D002878:Chromosomes, Human, Pair 1; D002879:Chromosomes, Human, Pair 10; D002881:Chromosomes, Human, Pair 12; D002882:Chromosomes, Human, Pair 13; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D015342:DNA Probes; D005786:Gene Expression Regulation; D005796:Genes; D006801:Humans; D006822:Hybrid Cells; D007370:Interferon Type I / Q000502:physiology*; D008970:Molecular Weight; D011544:Pseudogenes; D015347:RNA Probes; D015183:Restriction Mapping
D015342:DNA Probes; D007370:Interferon Type I; D015347:RNA Probes; D004247:DNA; D015088:2',5'-Oligoadenylate Synthetase