Drug and radiation sensitivity testing of primary human tumor cells using the adhesive-tumor-cell culture system (ATCCS).
In summary, the ATCCS is an efficient culture system which grows clonogenic cells from greater than 80% of human cancers. The ATCCS supports the growth of malignant cells from human cancers. The ATCCS shows classical drug and radiation survival curves which routinely achieve over 1 log of kill. The ATCCS has high CFE and permits sensitivity testing from small samples. Clinical correlations for the chemosensitivity assay were satisfactory. Radiosensitivity in vitro correlates with tumor histology.
Baker
F L
FL
Department of Hematology, U.T. M.D. Anderson Hospital and Tumor Institute, Houston 77030.
Spitzer
G
G
Ajani
J A
JA
Brock
W A
WA
D002448:Cell Adhesion; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D004354:Drug Screening Assays, Antitumor / Q000379:methods*; D006801:Humans; D011836:Radiation Tolerance; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000528:radiation effects