Infection of a human leukemia K-562 cell line with Semliki Forest virus.
Infection of the human leukemia hematopoietic stem cell line, K-562, with Semliki Forest virus (SFV) can be characterized by three stages: (1) an early virus-proliferating stage lasting 1 to 4 days post-infection (pi) in which infectious virus is produced in high titers (10(3)pfu/cell) but in which there is minimal cytopathic effect. All cells appear viable by trypan blue dye exclusion, although they do not proliferate, and DNA and cell protein synthesis decrease to less than 3% of uninfected controls within 24 hours; (2) an intermediate stage extending from day 5 to about day 24-30 pi in which the amount of infectious virus declines to low levels. During this stage, viral protein synthesis decreases to undetectable levels, although viral gylcoproteins are readily demonstrated by immunofluorescence and by immunoblot; however, capsid protein appears to degrade within 21 days pi. Cell numbers remain constant but the viability of the non-proliferating cells determined by trypan blue exclusion could not be determined with confidence; (3) a final long-term stage in which viral glycoproteins, E1 and E2, are detectable by immunoblots and immunofluorescence for many months but the cells are metabolically inactive and do not synthesize viral proteins. These non-viable cells do not lyse for as long as 2 years.
King
D D
DD
Department of Microbiology, College of Liberal Arts, University of Tennessee, Knoxville.
Brady
B
B
Dodd
D
D
Wust
C J
CJ
Brown
A
A
D000956:Antigens, Viral / Q000032:analysis; D015153:Blotting, Western; D003588:Cytopathogenic Effect, Viral; D004247:DNA / Q000096:biosynthesis; D006801:Humans; D014176:Protein Biosynthesis; D012672:Semliki forest virus / Q000254:growth & development* / Q000276:immunology; D013997:Time Factors; D014407:Tumor Cells, Cultured / Q000382:microbiology*; D014779:Virus Replication
D000956:Antigens, Viral; D004247:DNA