Neonatal myosin heavy chains are not expressed in Ni-induced rat rhabdomyosarcoma.
Myosin heavy chain (MHC) composition of chemically-induced rhabdomyosarcoma (RMS) was analyzed by gel electrophoresis and Western blotting using a panel of monoclonal antimyosin antibodies specific for embryonic-, neonatal-, slow- and adult fast-type MHC isoforms. Myosin extracted from tumours and electrophoresed on 6%-sodium dodecyl sulfate (SDS)glycerol gels was found to migrate as three distinct MHC components. These polypeptides were present in different relative amounts in the five RMS studied. Western blotting experiments revealed that variable proportions of embryonic-, slow- and adult fast-, but not neonatal-type, MHC isoforms are consistently expressed in RMS. Indirect and double immunofluorescence procedures applied to cryosections of tumoral tissue showed that: (a) RMS cells were unreactive with antineonatal-type-MHC antibody, (b) the majority of neoplastic, desmin-positive, cells contained embryonic- as well as adult fast-type MHCs and (c) a minority of cells were labelled by anti-slow MHC antibody. The results of this study indicate that there is no obligatory sequence of MHC isoform expression in the molecular transition (emb----neo----adult) which occurs during rat skeletal myogenesis.
Borrione
A C
AC
Institute of General Pathology, University of Padova, Italy.
Zanellato
A M
AM
Saggin
L
L
Mazzoli
M
M
Azzarello
G
G
Sartore
S
S
D000818:Animals; D000831:Animals, Newborn; D000911:Antibodies, Monoclonal; D007150:Immunohistochemistry; D009218:Myosins / Q000276:immunology / Q000378:metabolism*; D009532:Nickel; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012208:Rhabdomyosarcoma / Q000139:chemically induced / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D012333:RNA, Messenger; D009532:Nickel; D009218:Myosins