Nucleotide sequence of rat liver iodothyronine 5'-monodeiodinase (5' MD): its identity with the protein disulfide isomerase.
We report here the isolation and sequence of a near full length cDNA clone for the 5'MD. Screening of gt11 cDNA library with a 32P-labeled partial 5'MD clone (#23) yielded two further clones (#2301 and 2302). Clone 2301 was contained entirely within clone 23 while clone 2302 contained 0.5 kb upstream of 5' end and 1.0 kb downstream 3' end of clone 23. Clone 2302 has an open reading frame of 1,447 nucleotides followed by a stop codon and 584 nucleotides of the untranslated 3' end region. The predicted amino acid sequence showed a 99% and a 95% identity with protein disulfide isomerase (PDI) and the membrane associated thyroid hormone binding protein (MTHBP), respectively. Monoclonal antibodies against human placental PDI (HP13) neutralized the 5'MD and showed only one band in western blot analysis of rat liver solubilized microsomal proteins. The results suggest that clone 2302, MTHBP and PDI may be the same protein and that it represents 5' MD.
Boado
R J
RJ
Department of Medicine, University of California School of Medicine, Los Angeles 90024.
Campbell
D A
DA
Chopra
I J
IJ
D019747:Aldose-Ketose Isomerases; D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D002238:Carbohydrate Epimerases / Q000235:genetics*; D003001:Cloning, Molecular; D003062:Codon; D007453:Iodide Peroxidase / Q000235:genetics*; D008099:Liver / Q000201:enzymology*; D008969:Molecular Sequence Data; D051381:Rats
D003062:Codon; D007453:Iodide Peroxidase; D002238:Carbohydrate Epimerases; D019747:Aldose-Ketose Isomerases; C019715:L-arabinose isomerase