title: Tetrodotoxin in two species of ribbon worm (Nemertini), Lineus fuscoviridis and Tubulanus punctatus.
abstract: Extracts of two species of ribbon worm, Lineus fuscoviridis and Tubulanus punctatus, had lethal potencies in mice from 15-503 MU/g of worm and from less than 10-540 MU/g, respectively. The toxins were partially purified by ultrafiltration and column chromatography using Bio-Gel P-2 and Bio-Rex 70 (H+ form). Thin-layer chromatographic, high performance liquid chromatographic and gas chromatography-mass spectrometric analyses revealed the presence of tetrodotoxin and anhydrotetrodotoxin plus some unidentified compounds.
authors.affiliation: Faculty of Applied Biological Science, Hiroshima University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Miyazawa
authors.forename: M
authors.initials: M
authors.lastname: Higashiyama
authors.forename: K
authors.initials: K
authors.lastname: Ito
authors.forename: T
authors.initials: T
authors.lastname: Noguchi
authors.forename: O
authors.initials: O
authors.lastname: Arakawa
authors.forename: Y
authors.initials: Y
authors.lastname: Shida
authors.forename: K
authors.initials: K
authors.lastname: Hashimoto
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D008297:Male; D008387:Marine Toxins / Q000032:analysis* / Q000633:toxicity; D051379:Mice; D010986:Platyhelminths / Q000378:metabolism*; D013779:Tetrodotoxin / Q000032:analysis* / Q000633:toxicity
chemical_list: D008387:Marine Toxins; D013779:Tetrodotoxin