Different processing of chromogranin B into GAWK-immunoreactive fragments in the bovine adrenal medulla and pituitary gland.
Chromogranin B 420-493 (GAWK)-like immunoreactivity (chromogranin B (420-493)-LI) was determined by radioimmunoassay using two different rabbit antisera, one raised against chromogranin B (420-436) (GAWK 1-17) (Ab420-436) and the other against chromogranin B 439-457 (GAWK 20-38) (Ab439-457), in bovine and human tissues. Chromogranin B (420-493)-LI was present in the bovine adrenal medulla chromaffin granules as well as in the anterior pituitary gland and was released from the cultured bovine chromaffin cells by stimulation with high K+ or nicotine. Chromogranin B (420-493)-LI present in the bovine tissues was detected using Ab420-436 but was not detected using Ab439-457. In the human tissues, chromogranin B (420-493)-LI was detected using Ab420-436 as well as Ab439-457. This suggests that the amino acid sequence of this region (chromogranin B 439-457) is different between human and bovine. On the gel permeation chromatography, chromogranin B (420-493)-LI was eluted at the void volume in the bovine adrenal medulla and at an apparent molecular weight of 4000 in the anterior pituitary gland. On the reverse-phase high-performance liquid chromatography, multiple peaks of chromogranin B (420-493)-LI was detected in the bovine adrenal medulla while one component of chromogranin B (420-493)-LI was found in the anterior pituitary gland. These results suggest that chromogranin B is processed into small fragments of chromogranin B (420-493)-LIs and that this processing is tissue-specific.
Iguchi
H
H
Department of Biochemistry, National Kyushu Cancer Center, Fukuoka, Japan.
Natori
S
S
Kato
K
K
Nawata
H
H
Chrétien
M
M
D000313:Adrenal Medulla / Q000032:analysis / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D002837:Chromaffin Granules / Q000032:analysis / Q000378:metabolism; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D053378:Chromogranin B; D002864:Chromogranins / Q000032:analysis / Q000378:metabolism*; D006801:Humans; D007118:Immunoassay; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009538:Nicotine / Q000494:pharmacology; D010446:Peptide Fragments / Q000378:metabolism; D010903:Pituitary Gland, Anterior / Q000032:analysis / Q000378:metabolism*; D011188:Potassium / Q000494:pharmacology; D014018:Tissue Distribution
C506134:CHGB protein, human; D053378:Chromogranin B; D002864:Chromogranins; D009419:Nerve Tissue Proteins; D010446:Peptide Fragments; D009538:Nicotine; C044449:GAWK; D011188:Potassium