Disruption of the proto-oncogene int-2 in mouse embryo-derived stem cells: a general strategy for targeting mutations to non-selectable genes.
Gene targeting--homologous recombination of DNA sequences residing in the chromosome with newly introduced DNA sequences--in mouse embryo-derived stem cells promises to provide a means to generate mice of any desired genotype. We describe a positive nd negative selection procedure that enriches 2,000-fold for those cells that contain a targeted mutation. The procedure was applied to the isolation of hprt- and int-2- mutants, but it should be applicable to any gene.
Mansour
S L
SL
Howard Hughes Medical Institute, Department of Biology, University of Utah, Salt Lake City 84112.
Thomas
K R
KR
Capecchi
M R
MR
D000818:Animals; D002999:Clone Cells; D051379:Mice; D008822:Mice, Transgenic; D009154:Mutation; D011519:Proto-Oncogenes; D015183:Restriction Mapping; D013234:Stem Cells; D014162:Transfection