The complete amino acid sequence of bovine milk angiogenin.
The amino acid sequence of angiogenin isolated from bovine milk was deduced by gas-phase sequencing of the protein and its fragments. The protein contains 125 residues and has a calculated molecular mass of 14,577 Da. The sequence is highly homologous (65% identity) to the sequence of human angiogenin, most of the differences being the result of conservative replacements. Like human angiogenin, the bovine protein is also homologous to bovine pancreatic RNase A (34% identity) and the three major active site residues known to be involved in the catalytic process, His-14, Lys-41 and His-115, are conserved. When tested against conventional substrates for RNase A activity, bovine angiogenin displays the same selective ribonucleolytic activity as human angiogenin. The sequence of bovine angiogenin contains the cell recognition tripeptide Arg-Gly-Asp which is not present in the human protein.
Maes
P
P
Service de Chimie des Biomolécules, Institut Pasteur de Lille, France.
Damart
D
D
Rommens
C
C
Montreuil
J
J
Spik
G
G
Tartar
A
A
D000595:Amino Acid Sequence; D043925:Angiogenesis Inducing Agents; D000818:Animals; D002417:Cattle; D005260:Female; D006133:Growth Substances; D006801:Humans; D008892:Milk; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism; D010446:Peptide Fragments / Q000302:isolation & purification; D012259:Ribonuclease, Pancreatic; D012260:Ribonucleases / Q000378:metabolism; D013045:Species Specificity
D043925:Angiogenesis Inducing Agents; D006133:Growth Substances; D009363:Neoplasm Proteins; D010446:Peptide Fragments; D012260:Ribonucleases; C046366:angiogenin; D012259:Ribonuclease, Pancreatic