Levels of 7 alpha-hydroxy-4-cholesten-3-one in plasma reflect rates of bile acid synthesis in man.
A method for analysis of 7 alpha-hydroxy-4-cholesten-3-one in plasma is described. Following solid-phase extraction/purification the compound is determined by high-performance liquid chromatography using a UV detector. The median concentration in healthy subjects was 12 ng/ml (range 3-40). The levels were lower in diseases associated with a low bile acid production: extrahepatic cholestasis, less than 1.5 ng/ml (range less than 0.9-3); liver cirrhosis less than 1.5 ng/ml (range less than 0.9-38), and higher in diseases associated with a high bile acid production: cholestyramine treatment, 188 ng/ml (range 54-477); ileal resection 397 ng/ml (range 128-750). The levels were essentially normal in patients with colon resection. The results are consistent with a strong positive correlation between the levels of 7 alpha-hydroxy-4-cholesten-3-one in plasma and the rate of bile acid synthesis.
Axelson
M
M
Department of Clinical Chemistry, Karolinska Hospital, Stockholm, Sweden.
Aly
A
A
Sjövall
J
J
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001647:Bile Acids and Salts / Q000096:biosynthesis*; D015415:Biomarkers / Q000097:blood*; D002782:Cholestenes / Q000097:blood*; D002783:Cholestenones / Q000097:blood*; D002792:Cholestyramine Resin / Q000627:therapeutic use; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D007410:Intestinal Diseases / Q000097:blood; D008107:Liver Diseases / Q000097:blood; D008297:Male; D008875:Middle Aged
D001647:Bile Acids and Salts; D015415:Biomarkers; D002782:Cholestenes; D002783:Cholestenones; D002792:Cholestyramine Resin; C002656:7 alpha-hydroxy-4-cholesten-3-one