Transport of an iron: anthracycline complex by murine leukemia cells.
Cytotoxicity and transport of daunorubicin (DNR) and of a DNR: iron complex were examined, using P388 murine lymphoblasts and P388/ADR, an anthracycline-resistant sub-line. The drug: iron complex was dissociated in the presence of serum or, in serum-free medium, at the cell surface. Moreover, DNR toxicity was not promoted when the drug was provided as an iron complex. While formation of a complex with iron can markedly potentiate reactivity of anthracyclines in cell-free systems, these complexes play a role in cytotoxicity of DNR only if generated in the intracellular environment.
Kessel
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Department of Pharmacology, Wayne State University, School of Medicine Detroit, MI 48201.
D000818:Animals; D001692:Biological Transport; D003630:Daunorubicin / Q000378:metabolism*; D004351:Drug Resistance; D007501:Iron / Q000378:metabolism*; D007942:Leukemia, Experimental / Q000378:metabolism*; D051379:Mice; D014407:Tumor Cells, Cultured
D007501:Iron; D003630:Daunorubicin