The detection of conventional class I and class II I-E homologue major histocompatibility complex molecules on feline cells.
The presence on feline cells of class I and class II I-E type major histocompatibility complex (MHC) homologues was demonstrated using cross-reacting monoclonal antibodies (mAb). The feline class I antigen homologues were detected with both immunofluorescent and biochemical techniques, using the anti-human class I mAb W6/32. The class I antigens were detected on in vitro cultured feline fibroblasts and lymphoid cells, but not on fresh lymphoid cells, apparently as a result of the association of bovine beta-2 microglobulin with feline class I heavy chains which generated the determinant(s) recognized by mAb W6/32. Class II I-E-like molecules could be detected with immunofluorescent techniques using the species cross-reactive anti-mouse I-E antibody 40D only when peripheral blood mononuclear cells were activated, for example, with the mitogens staphylococcus enterotoxin A or lipopolysaccharide. The predominant expression of I-A-like molecules by resting class II-positive feline cells could explain some of the functional difference we have seen in comparison with those of most other mammalian species.
Pollack
M S
MS
Department of Microbiology and Immunology, Baylor College of Medicine, Houston, TX 77030.
Hayes
A
A
Mooney
S
S
Pedersen
N C
NC
Cook
R G
RG
D000818:Animals; D000911:Antibodies, Monoclonal; D002415:Cats / Q000276:immunology*; D003429:Cross Reactions; D015395:Histocompatibility Antigens Class I / Q000302:isolation & purification*; D000949:Histocompatibility Antigens Class II / Q000302:isolation & purification*; D007963:Leukocytes, Mononuclear / Q000276:immunology; D013045:Species Specificity
D000911:Antibodies, Monoclonal; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II