Bovine brain astrocytes express basic fibroblast growth factor, a neurotropic and angiogenic mitogen.
Astrocytes derived from adult bovine corpus callosum contain large amounts of mitogenic activity for capillary endothelial cells and astrocytes. On the basis of radioimmunoassay, bioassay and immunoneutralization studies, 99.5% of this activity consists of basic fibroblast growth factor (bFGF). Immunoblot and gene expression analysis are also consistent with the synthesis of bFGF. The remainder of bioactivity [0.5%] is accounted for by acidic fibroblast growth factor (aFGF). These data suggest that astrocytes are a source of brain-derived bFGF and to a much lesser extent also of aFGF. Considering that bFGF has been proposed to play a role in the development and differentiation of the nervous system through its angiogenic and neurotropic properties, its presence in astrocytes provides a rationale for the complex regulatory and neurotropic functions attributed to these cells. Also, the ability of bFGF to stimulate the growth of astrocytes themselves suggest a role for the mitogen in normal and aberrant proliferation of astrocytes.
Ferrara
N
N
Cancer Research Institute, University of California Medical Center, San Francisco 94143.
Ousley
F
F
Gospodarowicz
D
D
D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003337:Corpus Callosum / Q000166:cytology / Q000378:metabolism*; D005346:Fibroblast Growth Factors / Q000378:metabolism*; D008297:Male; D008934:Mitogens / Q000378:metabolism* / Q000494:pharmacology; D013997:Time Factors
D008934:Mitogens; D005346:Fibroblast Growth Factors