Inhibitors of sterol synthesis. Exogenous oleate reduces the inhibitory effect of 5 alpha-cholest-8(14)-en-3 beta-ol-15-one on the growth of CHO-K1 cells.
5 alpha-Cholest-8(14)-en-3 beta-ol-15-one, a hypocholesterolemic agent and a potent inhibitor of sterol biosynthesis, inhibited the growth of CHO-K1 cells incubated in medium containing fetal calf serum. The concentration of the oxysterol required to inhibit growth by 50% was 13 microM. Sodium oleate (82 microM) reduced the inhibitory effects of the sterol, and increased the concentration of the 15-ketosterol required to cause a 50% inhibition of growth to 25 microM. The ACAT inhibitor N'-(2,4-difluoro-phenyl)-N-[4-(2,2-dimethylpropy)-phenyl]-methyl)- N- heptylurea (5 microM) abolished the effect of sodium oleate, and reduced the concentration of the 15-ketosterol required to inhibit growth by 50% to 5 microM.
Pinkerton
F D
FD
Department of Biochemistry, Rice University, Houston, Texas 77251.
Kirkpatrick
N D
ND
Schroepfer
G J
GJ
D000818:Animals; D000924:Anticholesteremic Agents; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002782:Cholestenes / Q000494:pharmacology*; D002783:Cholestenones / Q000494:pharmacology*; D006224:Cricetinae; D005260:Female; D019301:Oleic Acid; D009829:Oleic Acids / Q000494:pharmacology*; D010053:Ovary; D014176:Protein Biosynthesis; D002785:Sterol O-Acyltransferase / Q000037:antagonists & inhibitors; D013261:Sterols / Q000037:antagonists & inhibitors*
D000924:Anticholesteremic Agents; D002782:Cholestenes; D002783:Cholestenones; D009829:Oleic Acids; D013261:Sterols; D019301:Oleic Acid; C013173:osteum; C015073:cholest-8(14)-en-3-ol-15-one; D002785:Sterol O-Acyltransferase