Chemotactic response of embryonic limb bud mesenchymal cells and muscle-derived fibroblasts to transforming growth factor-beta.
Transforming growth factor beta (TGF-beta) was tested for its ability to stimulate a chemotactic response in Stage 24 embryonic chick limb bud mesenchymal cells and muscle-derived fibroblasts. TGF-beta stimulated dose-dependent chemotaxis in both cell populations. Maximal chemotaxis was achieved with a concentration of 5 ng/ml for limb bud cells and as low as 15 pg/ml for muscle-derived fibroblasts. TGF-beta was not chemokinetic at these levels. Several other proteins found in bone, namely fibronectin, type I collagen, and osteonectin, were not chemotactic. However, both Bone Gla-protein and basic-FGF were found to be chemotactic but less effective than TGF-beta. Comparison with extracts of adult bone indicates that while TGF-beta is a potent chemoattractant, it does not account for all the chemotactic activity found in adult bone.
Lucas
P A
PA
Department of Biology, Case Western Reserve University, Cleveland, OH 44106.
Caplan
A I
AI
D000818:Animals; D001857:Bone Matrix / Q000032:analysis; D002478:Cells, Cultured; D002633:Chemotaxis / Q000187:drug effects*; D002642:Chick Embryo; D005121:Extremities / Q000166:cytology / Q000187:drug effects* / Q000196:embryology; D005347:Fibroblasts / Q000187:drug effects; D009132:Muscles / Q000166:cytology; D015291:Transforming Growth Factors / Q000032:analysis / Q000494:pharmacology*
D015291:Transforming Growth Factors