A site-specific single strand endonuclease activity induced by NYs-1 virus infection of a Chlorella-like green alga.
A site-specific endonuclease was isolated from a eukaryotic Chlorella-like green alga infected with the dsDNA-containing virus NYs-1. The enzyme recognizes the sequence 5'-CC-3' and cleaves 5' to the first C. It cleaves 5'-CmC-3' sequences but not 5'-mCC-3' sequences. The enzyme creates breaks in dsDNA whenever two 5'-CC-3' sequences on opposite strands are close enough for the two strands to separate; when the 5'-CC-3' sequences on opposite strands are further apart only a portion of the strands separate. Consequently, NYs-1 endonuclease does not produce a completely stable DNA digestion pattern. The enzyme probably does not cleave ssDNA and definitely does not cleave ssRNA or dsRNA.
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Department of Plant Pathology, University of Nebraska, Lincoln 68583-0722.
Morgan
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Schildkraut
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Van Etten
J L
JL
D002708:Chlorella / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D004277:DNA, Single-Stranded; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D015257:DNA-Cytosine Methylases / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000302:isolation & purification; D004790:Enzyme Induction; D008745:Methylation; D010942:Plant Viruses / Q000201:enzymology* / Q000235:genetics
D004277:DNA, Single-Stranded; D004279:DNA, Viral; D015257:DNA-Cytosine Methylases