[Safety of separation of maternal and fetal tissue in chorionic biopsy material as a prerequisite of prenatal gene diagnosis].
198 tissue samples from over all 336 chorionic biopsies obtained by different techniques had been examined histologically. Decidual residues could be found in 37 per cent of the specimens in spite of a twofold estimation of vitality. In correlation our increasing experience the frequency of decidual contamination decreased from 60 to 17 per cent. At the end of our examinations in only 5 per cent of 1,998 microscopic visual fields decidua could be found. This decidual contamination may be reason for misinterpretation of results. This error of the method has to be taken into consideration in all prenatal diagnostic statements especially in DNA- and enzymatic diagnostics.
Weise
W
W
Klinik für Gynäkologie und Geburtshilfe, Medizinischen Akademie Magdeburg.
Kersten
I
I
Erbstösser
G
G
D001706:Biopsy; D001707:Biopsy, Needle; D002823:Chorion / Q000033:anatomy & histology*; D002874:Chromosome Mapping; D003656:Decidua / Q000166:cytology; D005260:Female; D005819:Genetic Markers; D006801:Humans; D011247:Pregnancy; D011296:Prenatal Diagnosis / Q000379:methods*; D014327:Trophoblasts / Q000166:cytology
D005819:Genetic Markers
Zur Sicherheit der Trennung von mütterlichem und fetalem Gewebe im Chorionbioptat als Voraussetzung zur pränatalen Gendiagnostik.