title: Involvement of tumor necrosis factor in endotoxin-triggered neutrophil adherence to sinusoidal endothelial cells of mouse liver and its modulation in acute phase.
abstract: Tumor necrosis factor (TNF) has been shown to mediate lipopolysaccharide-induced neutrophil adhesion to liver sinusoidal endothelium in vivo. Female NMRI mice received either 5 micrograms lipopolysaccharide (R595) per animal alone (model A) or together with 116 mumol D-galactosamine (model B). One hour after injection, TNF activity in the serum was detectable to an equal extent in both models. Neutrophils in the liver, which had been identified by chloroacetate esterase staining of liver sections and quantitated by light microscopy, started to increase at 1 h and were elevated 10-fold above baseline at 6 h after application in (A) and (B). If 0.5 micrograms TNF instead of lipopolysaccharide was injected alone (model C) or together with D-galactosamine (model D), neutrophil influx into the liver was comparable to that observed in (A) or (B). Alanine aminotransferase activity in the serum was nearly normal in (A) and (C) 6 h after injection, while it reached levels up to 50-fold above baseline in models (B) and (D). This reflects the well-known D-galactosamine sensitization against lipopolysaccharide or TNF. Furthermore, degranulation of a large number of intrasinusoidal neutrophils could be observed 9 h after lipopolysaccharide-galactosamine injection. The administration of 116 mumol D-galactosamine per animal alone led neither to a measurable TNF activity in the serum nor to an increase in alanine aminotransferase activity or number of liver neutrophils. If the animals had received 50 microliter turpentine subcutaneously 24 h prior to lipopolysaccharide, TNF or D-galactosamine injection, the induced acute-phase reaction suppressed the increase of liver neutrophils in all models. Acute-phase reaction also prevented neutrophil degranulation and the rise of alanine aminotransferase in (B) to a great extent, while serum TNF activity was only minimally affected. It is concluded that TNF mediates neutrophil adhesion to the sinusoidal endothelium in vivo and that acute-phase reactants prevent lipopolysaccharide- or TNF-induced neutrophil influx into the liver.
authors.affiliation: Biochemisches Institut, Albert-Ludwigs-Universität, Freiburg i. Br., F.R.G.
authors.forename: H J
authors.initials: HJ
authors.lastname: Schlayer
authors.forename: H
authors.initials: H
authors.lastname: Laaff
authors.forename: T
authors.initials: T
authors.lastname: Peters
authors.forename: M
authors.initials: M
authors.lastname: Woort-Menker
authors.forename: H C
authors.initials: HC
authors.lastname: Estler
authors.forename: U
authors.initials: U
authors.lastname: Karck
authors.forename: H E
authors.initials: HE
authors.lastname: Schaefer
authors.forename: K
authors.initials: K
authors.lastname: Decker
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D004727:Endothelium / Q000166:cytology; D004731:Endotoxins / Q000633:toxicity*; D005260:Female; D008070:Lipopolysaccharides / Q000494:pharmacology; D008099:Liver / Q000166:cytology* / Q000473:pathology; D051379:Mice; D008815:Mice, Inbred Strains; D009504:Neutrophils / Q000187:drug effects*; D014409:Tumor Necrosis Factor-alpha / Q000502:physiology*
chemical_list: D000276:Adjuvants, Immunologic; D004731:Endotoxins; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D000410:Alanine Transaminase