The effect of nonadec(en)ylresorcinol on the fluidity of liposome and erythrocyte membranes.
The effect of alk(en)ylresorcinol homologs (5-(n-nonadecyl)- and 5-(n-nonadecenyl)resorcinol) on the mobility of 5-doxyl- and 12-doxylstearate spin probes incorporated into DMPC, DMPC-cholesterol and erythrocyte membranes was studied. It was found that both homologs affect the properties of hydrophobic environment of the membranes: (1) In DMPC vesicles both homologs induce an increase in the order parameter of 5-doxylstearate at temperatures of Tc and above. (2) At higher concentrations of both homologs a decrease in mobility of the 12-doxylstearate was also observed. (3) In the presence of cholesterol in the liposome membrane the influence of alk(en)ylresorcinols on the mobility of spin probes was much greater, depending on the cholesterol content and the position of the probe in the bilayer. (4) In natural membranes (erythrocyte ghosts) both alkyl- and alkenylresorcinols induced a decrease of mobility in the region of 12-doxylstearate as well as in the region closer to the polar head groups of lipids (5-doxylstearate).
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Instytut Biochemii, Uniwersytet Wrocławski, Poland.
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Sikorski
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D002784:Cholesterol / Q000502:physiology; D004134:Dimyristoylphosphatidylcholine; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000502:physiology; D006801:Humans; D066298:In Vitro Techniques; D008051:Lipid Bilayers; D008081:Liposomes; D008560:Membrane Fluidity / Q000187:drug effects*; D008563:Membrane Lipids / Q000502:physiology*; D012118:Resorcinols / Q000494:pharmacology*; D013113:Spin Labels; D013696:Temperature
D008051:Lipid Bilayers; D008081:Liposomes; D008563:Membrane Lipids; D012118:Resorcinols; D013113:Spin Labels; C057537:5-(n-nonadecyl)resorcinol; C057538:5-(n-nonadecenyl)resorcinol; D002784:Cholesterol; D004134:Dimyristoylphosphatidylcholine