Effects of cyclophosphamide and a metabolite, acrolein, on Naegleria fowleri in vitro and in vivo.
Mice challenged intranasally with Naegleria fowleri died of primary amoebic meningoencephalitis. Mice given 30 mg of cyclophosphamide per kg of body weight daily for 10 days starting 2 days before challenge were protected. Neither cyclophosphamide nor serum from cyclophosphamide-treated mice inhibited N. fowleri in vitro. A metabolic product of cyclophosphamide, acrolein, inhibited growth and enflagellation of N. fowleri. Acrolein at 40 microM was amoebicidal. Acrolein injured starved cells and amoebae at 5 degrees C and growing N. fowleri.
Zhang
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L
Department of Microbiology and Immunology, Virginia Commonwealth University, Richmond 23298-0110.
Marciano-Cabral
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F
Bradley
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SG
D000171:Acrolein / Q000494:pharmacology*; D000447:Aldehydes / Q000494:pharmacology*; D000562:Amebiasis / Q000188:drug therapy; D000818:Animals; D003520:Cyclophosphamide / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D008590:Meningoencephalitis / Q000188:drug therapy; D051379:Mice; D009253:Naegleria / Q000187:drug effects* / Q000254:growth & development; D013997:Time Factors
D000447:Aldehydes; D000171:Acrolein; D003520:Cyclophosphamide