Inhibition of dietary fat promoted development of (pre)neoplastic lesions in exocrine pancreas of rats and hamsters by supplemental selenium and beta-carotene.
The modulating effects of selenium and beta-carotene on the development of putative preneoplastic foci in exocrine pancreas were investigated in rats and hamsters, treated with azaserine and N-nitrosobis(2-oxopropyl)amine (BOP), respectively. The animals were fed a semipurified diet high in saturated fat (20% lard) either supplemented or not with beta-carotene or selenium. A separate group maintained on a diet low in saturated fat (5% lard) was incorporated as an extra control group. The animals were given their diets 12 days after the last treatment with carcinogen. Four months postinitiation, the pancreata were quantitatively examined for the number and size of putative (pre)neoplastic lesions. Rats as well as hamsters maintained on a low-fat diet had significantly less putative preneoplastic pancreatic lesions as compared to animals fed a diet containing 20% lard. Selenium and beta-carotene both inhibited the growth of basophilic foci in rat pancreas. In hamster pancreas, beta-carotene and selenium caused a significant decrease in the number of early ductal complexes. The number of carcinomas was decreased in the beta-carotene group and increased in the group fed a diet high in selenium.
Woutersen
R A
RA
Department of Biological Toxicology, TNO-CIVO Toxicology and Nutrition Institute, Zeist, Netherlands.
van Garderen-Hoetmer
A
A
D000818:Animals; D001377:Azaserine / Q000494:pharmacology; D002338:Carotenoids / Q000008:administration & dosage*; D006224:Cricetinae; D004032:Diet; D004041:Dietary Fats / Q000009:adverse effects*; D008297:Male; D008647:Mesocricetus; D009602:Nitrosamines / Q000494:pharmacology; D010190:Pancreatic Neoplasms / Q000473:pathology / Q000517:prevention & control*; D011230:Precancerous Conditions / Q000473:pathology / Q000517:prevention & control*; D051381:Rats; D011919:Rats, Inbred Strains; D012643:Selenium / Q000008:administration & dosage*; D019207:beta Carotene
D004041:Dietary Fats; D009602:Nitrosamines; D019207:beta Carotene; D002338:Carotenoids; C013622:nitrosobis(2-oxopropyl)amine; D001377:Azaserine; D012643:Selenium