Cytotoxic effects of müllerian inhibiting substance (MIS)?
To determine whether Müllerian Inhibiting Substance (MIS) is the factor responsible for the cytotoxic effects of testicular preparations on gynecological cancers, homogenates of bovine fetal testes were fractionated by Sephadex G 200 chromatography and their cytotoxic effects tested on a Müllerian-derived endometrial cancer cell line (AN3-CA), an ovarian carcinoma cell line (CAOV 3) and on a breast cancer cell line (MCF 7). No difference in cytotoxicity was found between Müllerian and non-Müllerian derived human cancer cells. Cytotoxicity and MIS activity were found in different fractions on Sephadex G 200 gel chromatography.
Janssens
J P
JP
Department of Gynecological Oncology, University of Leuven, Belgium.
Noto
V
V
van Tendeloo
G
G
Bonte
J
J
de Loecker
W
W
D054304:Anti-Mullerian Hormone; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D005260:Female; D005833:Genital Neoplasms, Female; D006023:Glycoproteins; D006131:Growth Inhibitors; D006801:Humans; D009095:Mullerian Ducts; D013735:Testicular Hormones / Q000494:pharmacology*
D006023:Glycoproteins; D006131:Growth Inhibitors; D013735:Testicular Hormones; D054304:Anti-Mullerian Hormone