A liver-specific chromatin protein binding to the 5' far upstream region of the rat serum albumin gene.
Chromatin proteins which were extracted with 0.3 M NaCl from rat liver, brain, and kidney nuclei were examined by the protein blotting technique for their ability to bind to the 5' upstream regions of the rat serum albumin gene. A 110-kDa protein from liver nuclei bound specifically to the most upstream fragment (between approximately equal to -7.3 kbp and -2.0 kbp from the cap site) of the cloned albumin genomic DNA, whereas no proteins from kidney and brain bound to this fragment. It is possible that the 110-kDa protein is concerned with the tissue-specific expression of the albumin gene.
Kenmochi
N
N
Department of Biochemistry, Niigata University School of Medicine, Japan.
Kuwano
Y
Y
Ogata
K
K
D000818:Animals; D001923:Brain Chemistry; D002843:Chromatin / Q000032:analysis*; D004268:DNA-Binding Proteins / Q000032:analysis; D005786:Gene Expression Regulation; D007668:Kidney / Q000032:analysis; D008099:Liver / Q000032:analysis*; D051381:Rats; D012709:Serum Albumin / Q000032:analysis / Q000235:genetics*; D014158:Transcription, Genetic
D002843:Chromatin; D004268:DNA-Binding Proteins; D012709:Serum Albumin