title: Thyroid status influences calcium ion accumulation and retention by rat liver mitochondria.
abstract: Ca2+ accumulation and retention by isolated rat liver mitochondria (RLM), measured with a Ca2+ electrode as aCa, are markedly influenced by thyroid status. RLM from propylthiouracil (PTU)-fed rats took up Ca2+ from a suspending medium (SM) until 10.26 +/- 2.51 (SD) x 10(-5) M Ca2+ had been added (n = 5). RLM from PTU rats given T3 (100 micrograms/kg daily x 6) showed uptake only until 2.37 +/- 0.59 x 10(-5) M Ca2+ had been added (n = 6) and RLM from normal rats showed uptake until 3.69 +/- 0.53 x 10(-5) M (n = 9) Ca2+ was reached. RLM from the three animal groups lowered the aCa in the SM from 9.13 +/- 1.69 x 10(-6) to 4.96 +/- 2.08 x 10(-6) M regardless of hormonal status. The time in minutes that the aCa remained below the initial level in the rat groups was PTU 94.8 +/- 26.2, PTU + T3, 11.5 +/- 3.9, and normal 26.7 +/- 3.8. All differences were significant at the 0.001 level (ANOVA). bGH did not affect Ca2+ handling by RLM from PTU rats. Administered T3 increased RLM alpha-glycerophosphate dehydrogenase activity 24-36 hr before normalizing Ca2+ handling. The thyroid hormone-sensitive system described here adjusts the SM Ca2+ concentration to a level far above cytosolic so that its function may be to regulate intramitochondrial [Ca2+]. The level of intramitochondrial Ca2+ may be of importance in the mechanism of action of thyroid hormone.
authors.affiliation: Department of Physiology, Cornell University Medical College, New York 10021.
authors.forename: R L
authors.initials: RL
authors.lastname: Greif
mesh_terms: D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002118:Calcium / Q000378:metabolism* / Q000494:pharmacology; D002122:Calcium Chloride / Q000494:pharmacology; D004563:Electrochemistry; D007037:Hypothyroidism / Q000378:metabolism / Q000503:physiopathology; D008297:Male; D008930:Mitochondria, Liver / Q000378:metabolism*; D011441:Propylthiouracil / Q000008:administration & dosage; D051381:Rats; D011919:Rats, Inbred Strains; D013961:Thyroid Gland / Q000502:physiology*; D014284:Triiodothyronine / Q000008:administration & dosage
chemical_list: D014284:Triiodothyronine; D011441:Propylthiouracil; D002122:Calcium Chloride; D002118:Calcium