Gene transcription in a temperature-sensitive mutant of RNA polymerase II.
We have studied the transcription rates and the steady-state levels of cytoplasmic mRNA of selected genes in tsAF8 cells, a temperature-sensitive mutant of RNA polymerase II. At the restrictive temperature, the transcription rates decrease uniformly for all genes examined, while the corresponding decreases in steady-state levels of cytoplasmic mRNA vary for individual genes. tsAF8 cells can be advantageously used to study the half-line of mRNA without the use of perturbing drugs.
Jaskulski
D
D
Department of Pathology, Temple University, School of Medicine, Philadelphia, PA 19140.
Baserga
R
R
D000199:Actins / Q000235:genetics; D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D012321:DNA-Directed RNA Polymerases / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D012333:RNA, Messenger / Q000378:metabolism; D013696:Temperature; D013937:Thymidine Kinase / Q000235:genetics; D014158:Transcription, Genetic; D014746:Vimentin / Q000235:genetics
D000199:Actins; D012333:RNA, Messenger; D014746:Vimentin; D013937:Thymidine Kinase; D012321:DNA-Directed RNA Polymerases