The evaluation of HBDH and LDH isoenzymes in cardiac cell necrosis of the rat.
The total LDH, HBDH and LDH isoenzyme activities were assessed in a number of rat tissues. HBDH did not correlate with LD1 and LD2 isoenzyme activity in tissues with high HBDH activity. HBDH therefore cannot be used as a marker for cardiac necrosis in the rat. In rats treated with isoprenaline and SK&F 94120 at doses producing myocardial necrosis, only the LD1 isoenzymes showed any significant change but only within the first 24 h of treatment. No statistically significant differences were seen in the plasma AST, CK, CK-MB and total LDH activities.
Barrett
R J
RJ
Smith Kline & French Research Ltd, Welwyn, Herts, UK.
Harleman
J H
JH
Joseph
E C
EC
D000818:Animals; D005260:Female; D006321:Heart / Q000187:drug effects; D006801:Humans; D006884:Hydroxybutyrate Dehydrogenase / Q000378:metabolism*; D007527:Isoenzymes; D007545:Isoproterenol / Q000494:pharmacology; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D008297:Male; D009206:Myocardium / Q000201:enzymology / Q000473:pathology*; D009336:Necrosis; D011719:Pyrazines / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D014018:Tissue Distribution
D007527:Isoenzymes; D011719:Pyrazines; C048350:5-(4-acetamidophenyl)pyrazin-2(1H)-one; D007770:L-Lactate Dehydrogenase; D006884:Hydroxybutyrate Dehydrogenase; D007545:Isoproterenol