title: Differentiation of cardiac baroreflex properties by cuff and drug methods in two rabbit strains.
abstract: Sigmoidal mean arterial pressure (MAP)-heart rate (HR) curves were obtained in two genetically related strains of rabbits (groups I and II). We examined vagal (V) and sympathetic (S) effects on HR using perivascular cuffs and vasoactive drugs to alter MAP, and we studied the effects of intravenous naloxone, which affected only I. With the cuff method, both V and S components of gain were much greater in I than in II, and naloxone greatly reduced the difference. With the drug method, gain was similar in I and II and unaffected by naloxone. With both methods, the V component of HR range between plateaus was greater in I than in II because of more pronounced bradycardia at the lower plateau; naloxone eliminated the latter difference when the reflex was drug induced but not when it was cuff induced. The S component of HR range was similar in I and II; with both methods, the tachycardia plateau was reduced by naloxone. The cuff method alters cardiac load more than the drug method, leading to engagement of different groups of afferents for a given change in MAP (delta MAP). We have derived an input-output model, which suggests that with the drug method, gain is almost entirely determined by the input from the arterial baroreceptors, accounting for the minimal difference between I and II. With the cuff method, gain is determined by a nonlinear interaction involving the arterial and nonarterial baroreceptors, which accentuates the response. The opiate mechanism is associated with the nonarterial baroreceptor input and has amplifying properties, which account for the difference in gain between I and II. The two methods and naloxone provide a novel way of differentiating the effector patterns of the reflex; naloxone serves as a marker of activity in a particular pathway.
authors.affiliation: Baker Medical Research Institute, Melbourne, Victoria, Australia.
authors.forename: M
authors.initials: M
authors.lastname: Weinstock
authors.forename: P I
authors.initials: PI
authors.lastname: Korner
authors.forename: G A
authors.initials: GA
authors.lastname: Head
authors.forename: P K
authors.initials: PK
authors.lastname: Dorward
mesh_terms: D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D004594:Electrophysiology / Q000379:methods; D006321:Heart / Q000294:innervation*; D006339:Heart Rate / Q000187:drug effects; D009270:Naloxone / Q000494:pharmacology*; D011311:Pressoreceptors / Q000187:drug effects / Q000502:physiology*; D011817:Rabbits; D012018:Reflex; D013045:Species Specificity; D013564:Sympathetic Nervous System / Q000502:physiology; D014630:Vagus Nerve / Q000502:physiology
chemical_list: D009270:Naloxone