Primary structure of the carboxy-terminal region of a higher plant beta-tubulin.
A tubulin-specific cDNA clone was isolated, using anti-chicken brain beta-tubulin monoclonal antibody as a probe, from a lambda gtll library of cDNA prepared from cultured carrot cells. It included a coding region of the C-terminal 39 amino acids and a part of the 3'-flanking of a beta-tubulin mRNA. The predicted amino acid sequence of 17 residues in the C-terminal variable region was AspGluGluGluTyrTyrGluAspGluGluGluGluGluAlaGlnGlyMet. Twenty-two amino acids preceding this acidic terminus were completely identical with those of the other known beta-tubulins, but the codons for them included many silent substitutions.
Okamura
S
S
Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, Japan.
Azumano
I
I
D000595:Amino Acid Sequence; D001483:Base Sequence; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis; D005796:Genes; D008969:Molecular Sequence Data; D010944:Plants / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D014404:Tubulin / Q000032:analysis / Q000235:genetics*
D012333:RNA, Messenger; D014404:Tubulin; D004247:DNA