Development of an HPLC assay for the analysis of tetrafluoroputrescine--a putrescine analog.
A simple reverse-phase liquid chromatographic system with ultraviolet detection at 254 nm for tetrafluoroputrescine (TFP), a putrescine analog, is described. The assay involves precolumn derivatization of TFP with dansyl chloride at pH 6.8-7.5 at 60 degrees C, followed by separation from putrescine (PUT) and quantitation. The derivatization procedure was adapted for the simultaneous analysis of TFP and PUT in whole blood components. Also, preliminary studies on protein binding of TFP are reported.
Hawi
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Division of Medicinal Chemistry, College of Pharmacy, University of Kentucky, Lexington 40536-0082.
Yip
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H
Sullivan
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TS
Digenis
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GA
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003619:Dansyl Compounds / Q000097:blood; D004912:Erythrocytes / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010949:Plasma / Q000032:analysis; D011485:Protein Binding; D011700:Putrescine / Q000031:analogs & derivatives* / Q000032:analysis / Q000097:blood; D051381:Rats; D012710:Serum Albumin, Bovine; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature
D003619:Dansyl Compounds; D012710:Serum Albumin, Bovine; C049506:2,2,3,3-tetrafluoroputrescine; D011700:Putrescine