Clofibrate does not induce peroxisomal 3 alpha,7 alpha,12 alpha-trihydroxy-5 beta-cholestanoyl coenzyme A oxidation in rat liver. Evidence that this reaction is catalyzed by an enzyme system different from that of peroxisomal acyl-coenzyme A oxidation.
The effect of clofibrate treatment of rats on the peroxisomal conversion in vitro of 3 alpha,7 alpha,12 alpha-trihydroxy-5 beta-cholestanoic acid into cholic acid in liver fractions has been investigated. No increase in the activity was observed after clofibrate treatment. In contrast, peroxisomal palmitate oxidation and palmitoyl-CoA oxidase activity increased several fold. It is concluded that the enzyme system responsible for the oxidative cleavage of the steroid side chain in bile acid formation is different from the enzyme system involved in the peroxisomal beta-oxidation of long chain fatty acids.
Pedersen
J I
JI
Institute for Nutrition Research, School of Medicine, University of Oslo, Norway.
Hvattum
E
E
Flatabø
T
T
Björkhem
I
I
D000818:Animals; D002384:Catalysis; D002793:Cholic Acids / Q000378:metabolism*; D002994:Clofibrate / Q000494:pharmacology*; D003065:Coenzyme A / Q000378:metabolism*; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008830:Microbodies / Q000187:drug effects / Q000378:metabolism*; D010084:Oxidation-Reduction / Q000187:drug effects; D010088:Oxidoreductases / Q000378:metabolism; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D002793:Cholic Acids; D010169:Palmitic Acids; D019308:Palmitic Acid; C003681:3,7,12-trihydroxycoprostanic acid; D010088:Oxidoreductases; C028427:palmitoyl CoA oxidase; D002994:Clofibrate; D003065:Coenzyme A