Mutation affecting peptide bond formation in nikkomycin biosynthesis.
Nikkomycin, a nucleoside-peptide analog of UDP-N-acetylglucosamine, is a potent chitin synthase inhibitor produced by the bacterium Streptomyces tendae. The HPLC profile of fermentation products in culture broths of a non-producing mutant, Nik 15, was compared with nikkomycin standards. Nikkomycin C and D, the glycone and aglycone moieties, respectively, of nikkomycin Z accumulated. This indicates the mutation affects the capacity to form a peptide bond between nikkomycin C and D, which is here proposed to be the terminal step in the synthesis of the biologically active nikkomycin Z. This is also the first documented case of a mutation affecting a specific step in nikkomycin biosynthesis.
Engel
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Southern Regional Research Laboratory, USDA, New Orleans, LA 70124.
Ullah
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AH
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D015394:Molecular Structure; D009154:Mutation; D010455:Peptides / Q000378:metabolism*; D013302:Streptomyces / Q000378:metabolism*
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D010455:Peptides; C011952:nikkomycin