Serologic comparison of avian reovirus isolates using virus neutralization and an enzyme-linked immunosorbent assay.
The serologic relatedness of six avian reovirus isolates (CO8, S1133, 81-5, 2408, 1733, and UMI 203) were determined using a virus-neutralization (VN) test and an enzyme-linked immunosorbent assay (ELISA). Six groups of 20 specific-pathogen-free broilers each were twice infected with one of the six isolates per group. Serum was reacted against each isolate in a beta VN test in chicken embryo kidney cells and against the S1133 virus in ELISA. Relatedness (R) values, determined by cross VN, revealed that all belonged to a single serotype. However, the CO8 isolate represented a major subtype difference compared with the other isolates. R values among the five other isolates indicated minor or no subtype differences. ELISA also showed major differences between the CO8 and the other isolates.
Giambrone
J J
JJ
Department of Poultry Science, Auburn University, Alabama 36849-5416.
Solano
W
W
D000818:Animals; D001717:Birds / Q000382:microbiology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D009500:Neutralization Tests / Q000662:veterinary; D012087:Reoviridae / Q000276:immunology*; D012703:Serotyping / Q000662:veterinary; D013045:Species Specificity; D014765:Viral Vaccines / Q000276:immunology*
D014765:Viral Vaccines