Rapid preparation of the nicotinic acetylcholine receptor for crystallization in detergent solution.
A novel rapid purification method for the nicotinic acetylcholine receptor from Torpedo electric tissue was developed. It allows preparation of 10 mg quantities of pure and stabile receptor protein within 2 days. This protein is used for crystallization attempts. Conditions are described which reproducibly yield crystals.
Hertling-Jaweed
S
S
Institut für Biochemie, Freie Universität Berlin, Germany.
Bandini
G
G
Müller-Fahrnow
A
A
Dommes
V
V
Hucho
F
F
D000818:Animals; D003460:Crystallization; D003902:Detergents; D004557:Electric Organ / Q000378:metabolism; D005960:Glucosides; D011978:Receptors, Nicotinic / Q000302:isolation & purification*; D012996:Solutions; D014101:Torpedo
D003902:Detergents; D005960:Glucosides; D011978:Receptors, Nicotinic; D012996:Solutions; C018619:octyl-beta-D-glucoside