Enzymatic sulfation of salivary mucins: structural features of 35S-labeled oligosaccharides.
Sulfotransferase activity catalyzing the transfer of sulfate ester group from 3'-phosphoadenosine-5'-phosphosulfate to salivary mucins was found associated with Golgi-rich membrane fraction of rat submandibular salivary glands. Alkaline borohydride reductive cleavage of the synthesized 35S-labeled glycoprotein led to the liberation of the label into reduced acidic oligosaccharides. Most of the label was found incorporated in four oligosaccharides. These were identified as sulfated tri-, penta-, hepta- and nonasaccharides. The trisaccharide was characterized as SO3H,6GlcNAc beta 1,3Gal beta 1,3GalNac-o1, the pentasaccharide as SO3H,6GlcNAc beta 1,3Gal beta 1,4GlcNAc beta 1,3(NeuAc alpha 2,6) GalNAc-o1 and heptasaccharide as SO3H,6Gal beta 1,4GlcNAc beta 1,6(Fuc alpha 1) 2Gal beta 1,4GlcNAc beta 1,3Gal beta 1,3)GalNAc-o1.
Slomiany
B L
BL
Dental Research Center, New Jersey Dental School, University of Medicine & Dentistry of New Jersey, Newark 07103-2425.
Piotrowski
J
J
Nishikawa
H
H
Slomiany
A
A
D000818:Animals; D008297:Male; D009077:Mucins / Q000378:metabolism*; D009844:Oligosaccharides / Q000378:metabolism; D051381:Rats; D013347:Subcellular Fractions / Q000201:enzymology; D013363:Submandibular Gland / Q000201:enzymology*; D013379:Substrate Specificity; D013431:Sulfates / Q000378:metabolism*; D015238:Sulfotransferases / Q000378:metabolism*
D009077:Mucins; D009844:Oligosaccharides; D013431:Sulfates; D015238:Sulfotransferases