The inhibition of the postnatal rise of 2,3-diphosphoglycerate in newborn lambs as a result of glucose perfusion.
Due to the abrupt increase in 2,3-diphosphoglycerate (2,3-DPG) concentration in the newborn lamb, which begins soon after birth, this interval in development was considered an excellent period to test the hypothesis that glucose perfusions could inhibit 2,3-DPG synthesis. Ten newborn lambs were divided into two groups and perfused either with glucose (15 mg/kg/min) or physiologic saline (45% NaCl) for 10 days. Blood gases, electrolytes, glycemia, O2 pressure at 50% Hb saturation, and 2,3-DPG levels were compared in the two groups. Glucose levels remained significantly elevated during the first 3 days in the glucose perfused group. The O2 pressure at 50% Hb saturation increased in both groups but was significantly lower in the glucose perfused group when determined on day 5 and 8. The postnatal increase in 2,3-DPG was significantly diminished in the glucose infused lambs, which suggests that glucose perfusion has an inhibiting effect on erythrocyte 2,3-DPG synthesis.
Bard
H
H
Department of Pediatrics, Université de Montréal, Quebec, Canada.
Fouron
J C
JC
Prosmanne
J
J
D019794:2,3-Diphosphoglycerate; D000818:Animals; D000831:Animals, Newborn / Q000097:blood*; D001786:Blood Glucose / Q000378:metabolism; D004163:Diphosphoglyceric Acids / Q000097:blood*; D005947:Glucose / Q000008:administration & dosage / Q000494:pharmacology*; D010100:Oxygen / Q000097:blood; D010477:Perfusion; D012756:Sheep
D001786:Blood Glucose; D004163:Diphosphoglyceric Acids; D019794:2,3-Diphosphoglycerate; D005947:Glucose; D010100:Oxygen