[Localization of fluorescent analogs of cholesterol and its esters in lipid models of membranes and lipoproteins].
The localization of the fluorescent cholesterol analogue--delta 5,7,9(11)-cholestatrien-3 beta-ol (B-CTE) and its methyl (M-CTE) and stearoyl (St-CTE) esters in model lipid particles were investigated by the radiationless energy transfer. It is shown that 67-100% of B-CTE molecules localize in the phospholipid monolayer of particles containing phosphatidylcholine and triolein (1:2 w/w). The replacement of a hydrogen atom in the hydroxyl by methyl resulted in immersion of 2/3 of the M-CTE molecules to the triolein core. This fact confirmed distribution of the fluorescent probe molecules in the whole volume of lipid particles. St-CTE was practically completely localized in the core of lipid particles. The results obtained evidence for the important role of 3-OH group in keeping the B-CTE molecules in the phospholipid monolayer of the investigated particles.
Kurek
N K
NK
Lapshin
E N
EN
Ioffe
D V
DV
Missiul'
B V
BV
D002782:Cholestenes; D002784:Cholesterol / Q000032:analysis*; D002788:Cholesterol Esters / Q000032:analysis*; D005456:Fluorescent Dyes; D008055:Lipids / Q000032:analysis; D008074:Lipoproteins / Q000032:analysis; D008081:Liposomes / Q000032:analysis*; D008962:Models, Theoretical; D011789:Quantum Theory
D002782:Cholestenes; D002788:Cholesterol Esters; D005456:Fluorescent Dyes; D008055:Lipids; D008074:Lipoproteins; D008081:Liposomes; C025498:cholesta-5,7,9-trien-3 beta-ol; D002784:Cholesterol
Issledovanie lokalizatsii fluorestsentnykh analogov kholesterina i ego éfirov v lipidnykh modeliakh membran i lipoproteinov.