Platelet-activating-factor-induced serum-albumin release from rabbit platelets in the absence of calcium.
Using a radioimmunoassay for rabbit-serum albumin, platelet-activating-factor-induced serum-albumin release by platelets was monitored under non-aggregating conditions. The four main results from this study are as follows. The EC50 of the release was of the same order of magnitude as the aggregation EC50 in the presence of calcium. The release took place within 2 min and was inhibited by BN 52021, which is a very specific inhibitor of the platelet-activating-factor-aggregating effect. Serum-albumin release was much greater than serotonin release.
Bette-Bobillo
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Laboratoire de Biologie Physico-Chimique, Unité Associée 530 du Centre National de la Recherche Scientifique, Université des Sciences et Techniques du Languedoc, Montpellier, France.
Bienvenue
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D000818:Animals; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism*; D002118:Calcium / Q000494:pharmacology*; D007700:Kinetics; D010972:Platelet Activating Factor / Q000494:pharmacology*; D011817:Rabbits; D011863:Radioimmunoassay; D012701:Serotonin / Q000097:blood; D012709:Serum Albumin / Q000378:metabolism*
D010972:Platelet Activating Factor; D012709:Serum Albumin; D012701:Serotonin; D002118:Calcium