Heterogeneous colony forming ability in vitro of the abnormal clone-derived granulocyte-macrophage precursors in myelodysplastic syndromes.
The clonal origin of granulocyte-macrophage colony forming cells (CFU-GM) in the myelodysplastic syndrome (MDS) was cytogenetically studied. Chromosome analysis was carried out on single GM-colonies from six patients with MDS, whose bone marrow cells had chromosome abnormalities. Abnormal clone-derived CFU-GM were grown in four patients under the presence of human placental conditioned medium. In the remaining two, all analysed colonies revealed a normal karyotype, although the majority of metaphase cells showed an abnormal karyotype in bone marrow preparations. These results indicate that the abnormal clone-derived CFU-GM in MDS have a clone-by-clone variation in colony forming ability in vitro.
Okuda
T
T
Third Department of Internal Medicine, Kyoto Prefectural University of Medicine, Japan.
Yokota
S
S
Maekawa
T
T
Sonoda
Y
Y
Misawa
S
S
Takino
T
T
Abe
T
T
D000328:Adult; D000368:Aged; D001853:Bone Marrow / Q000473:pathology; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D005260:Female; D006098:Granulocytes / Q000473:pathology*; D006412:Hematopoietic Stem Cells / Q000473:pathology*; D006801:Humans; D007621:Karyotyping; D008264:Macrophages / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D009190:Myelodysplastic Syndromes / Q000235:genetics / Q000473:pathology*