Alkane production by isolated rat heart and lung.
Ethane and pentane have been measured as an index of lipid peroxidation occurring in the isolated perfused rat heart as well as lung preparations. In order to initiate lipid peroxidation cumene hydroperoxide was infused. The results show a significant enhancement of both ethane and pentane evolution from both isolated organs by cumene hydroperoxide. The production of alkanes by perfusion of high doses of cumene hydroperoxide in lungs is about one-half that of the isolated heart. Such reduced production of alkanes by the lungs can be explained by the fact that lung lipids, which contain mostly saturated fatty acids, might have resisted the peroxidative degradation. In comparison to cumene hydroperoxide, tert-butyl hydroperoxide also increased alkane production, caused a reduction of glutathione content and increased oxidized glutathione in hearts. 4-Hydroxynonenal infusion was much less effective in causing alkane release but caused profound depletion of cardiac glutathione. Paraquat had relatively insignificant effects on cardiac lipid peroxidation and glutathione content.
Dutta
S
S
Department of Pharmacology, Wayne State University, Detroit, MI 48201.
Müller
A
A
Ishikawa
T
T
Zimmer
M
M
Sies
H
H
D000818:Animals; D001555:Benzene Derivatives / Q000378:metabolism / Q000494:pharmacology; D004980:Ethane / Q000096:biosynthesis*; D005978:Glutathione / Q000378:metabolism; D006321:Heart / Q000187:drug effects; D066298:In Vitro Techniques; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008168:Lung / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D010420:Pentanes / Q000096:biosynthesis*; D051381:Rats; D011919:Rats, Inbred Strains
D001555:Benzene Derivatives; D010420:Pentanes; C033353:pentane; D007770:L-Lactate Dehydrogenase; D005978:Glutathione; D004980:Ethane; C007164:cumene hydroperoxide