title: Conservation of amino acid sequences between human and porcine choline acetyltransferase.
abstract: The amino acid sequence of 11 peptides generated from human placental choline acetyltransferase was compared to the corresponding amino acid sequences predicted from the nucleotide sequence of a recently cloned porcine choline acetyltransferase cDNA. These peptides, which were generated by cyanogen bromide cleavage or tryptic digestion, accounted for 23% of the amino acids in the enzyme. Of the 145 amino acids sequenced eight differed between the two species, yielding an identity of 94% over the regions sampled. Of the eight amino acids that differed six could represent single base changes in the DNA sequence. These findings demonstrate strong sequence similarity between porcine and human choline acetyltransferase and indicate that they are closely related evolutionarily.
authors.affiliation: Department of Biochemistry, University of Texas, Southwestern Medical Center, Dallas 75235.
authors.forename: L B
authors.initials: LB
authors.lastname: Hersh
authors.forename: K
authors.initials: K
authors.lastname: Takane
authors.forename: K
authors.initials: K
authors.lastname: Gylys
authors.forename: C
authors.initials: C
authors.lastname: Moomaw
authors.forename: C
authors.initials: C
authors.lastname: Slaughter
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D002795:Choline O-Acetyltransferase; D003488:Cyanogen Bromide; D004247:DNA / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D010446:Peptide Fragments; D012689:Sequence Homology, Nucleic Acid; D013552:Swine; D014357:Trypsin
chemical_list: D010446:Peptide Fragments; D004247:DNA; D002795:Choline O-Acetyltransferase; D014357:Trypsin; D003488:Cyanogen Bromide