title: Structural analysis of the specific capsular polysaccharide of Streptococcus pneumoniae type 45 (American type 72).
abstract: The specific capsular polysaccharide of Streptococcus pneumoniae type 45 (American type 72) was found to be a high molecular weight polymer composed of D-galactose, 2-acetamido-2-deoxy-D-galactose, 2-acetamido-2-deoxy-D-glucose, 2-acetamido-2-deoxy-L-fucose, L-rhamnose, glycerol, and phosphate (2:1:1:1:1:1:1). Partial hydrolysis, dephosphorylation, methylation analysis, periodate oxidation studies, and one- and two-dimensional 1H and 13C high-field nuclear magnetic resonance experiments showed the polysaccharide to be a branched polymer of a 1-phosphoglycerol-substituted hexasaccharide repeating unit having the structure: (formula; see text).
authors.affiliation: Division of Biological Sciences, National Research Council, Ottawa, Ontario, Canada.
authors.forename: M
authors.initials: M
authors.lastname: Moreau
authors.forename: J C
authors.initials: JC
authors.lastname: Richards
authors.forename: M B
authors.initials: MB
authors.lastname: Perry
authors.forename: P J
authors.initials: PJ
authors.lastname: Kniskern
mesh_terms: D002240:Carbohydrate Sequence; D008401:Gas Chromatography-Mass Spectrometry; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D015394:Molecular Structure; D011135:Polysaccharides, Bacterial / Q000302:isolation & purification*; D013296:Streptococcus pneumoniae / Q000032:analysis*
chemical_list: D011135:Polysaccharides, Bacterial