title: Phenelfamycins, a novel complex of elfamycin-type antibiotics. II. Isolation and structure determination.
abstract: A novel complex of elfamycin-type antibiotics has been isolated from submerged fermentation of either Streptomyces violaceoniger AB 999F-80 or Streptomyces violaceoniger AB 1047T-33. Antibiotics were extracted from the fermentation broth with ethyl acetate and from the mycelia with acetone. Purification of individual components was achieved by a combination of solvent partitions, Sephadex LH-20 exclusion, C18 bonded-phase silica gel adsorption, diol partition and liquid-liquid countercurrent chromatographies. Seven closely related components were separated and assigned structures 4, 11, 12, 13, 14, and 16 to phenelfamycins A to F respectively and structure 17 to unphenelfamycin. These structures were elucidated employing a variety of spectroscopic techniques, including extensive use of 1D and 2D NMR spectroscopy.
authors.affiliation: Pharmaceutical Products Division, Abbott Laboratories, Abbott Park, Illinois 60064.
authors.forename: J E
authors.initials: JE
authors.lastname: Hochlowski
authors.forename: M H
authors.initials: MH
authors.lastname: Buytendorp
authors.forename: D N
authors.initials: DN
authors.lastname: Whittern
authors.forename: A M
authors.initials: AM
authors.lastname: Buko
authors.forename: R H
authors.initials: RH
authors.lastname: Chen
authors.forename: J B
authors.initials: JB
authors.lastname: McAlpine
mesh_terms: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents / Q000302:isolation & purification*; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D013056:Spectrophotometry, Ultraviolet; D013302:Streptomyces / Q000378:metabolism
chemical_list: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents