Strategy for molecular cloning of the fragile X site DNA.
Fragile X syndrome is a common form of mental retardation associated with a fragile site on the human X chromosome. We have recently demonstrated that the fragile X chromosome, when isolated within a somatic cell hybrid, often participates in translocations involving rodent chromosome arms. Cytogenetic and molecular evidence strongly suggests that the human breakpoint of these translocations is within the fragile X sequence. Hence, the joining of heterologous DNA (i.e. from two species) may permit the molecular cloning of the fragile X site. We describe here the cloning approach employed to enhance the isolation of interspecific chromosome translocation junctions. The human portion of the translocation junction should be derived from the fragile X site sequence.
Warren
S T
ST
Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia 30322.
Zhang
F P
FP
Sutcliffe
J S
JS
Peters
J F
JF
D000818:Animals; D003001:Cloning, Molecular; D003360:Cosmids; D004274:DNA, Recombinant; D005600:Fragile X Syndrome / Q000235:genetics*; D006801:Humans; D006822:Hybrid Cells; D012729:Sex Chromosome Aberrations / Q000235:genetics*; D014178:Translocation, Genetic; D014960:X Chromosome
D004274:DNA, Recombinant