title: Surface proteins of Staphylococcus aureus.
abstract: Crude cell walls of Staphylococcus aureus of potential immunologic or epidemiologic importance were prepared for study by mechanically disrupting whole cells and applying differential centrifugation. Cell-wall proteins were then released from the wall by lysostaphin digestion and analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Distinct electrophoretic protein patterns were found for each of the 12 serologically distinct strains examined. There were two major groups of cell-wall proteins identified in the 45-57-kilodalton (kdal) and 100-190-kdal range, respectively. Proteins in the 45-57-kdal range appeared to represent protein A variants of different apparent molecular mass. Localization experiments with 125I labeling demonstrated the surface location of most of the proteins. These data suggest that selected surface-exposed proteins may be important in defining host immune responses to S. aureus infections.
authors.affiliation: Department of Pediatrics, Harbor-UCLA Medical Center, Torrance.
authors.forename: A L
authors.initials: AL
authors.lastname: Cheung
authors.forename: A S
authors.initials: AS
authors.lastname: Bayer
authors.forename: J
authors.initials: J
authors.lastname: Peter
authors.forename: J I
authors.initials: JI
authors.lastname: Ward
mesh_terms: D001345:Autoradiography; D001426:Bacterial Proteins / Q000032:analysis / Q000302:isolation & purification*; D015153:Blotting, Western; D002473:Cell Wall / Q000032:analysis / Q000648:ultrastructure; D002499:Centrifugation, Density Gradient; D004591:Electrophoresis, Polyacrylamide Gel; D008565:Membrane Proteins / Q000032:analysis / Q000302:isolation & purification*; D008854:Microscopy, Electron; D013211:Staphylococcus aureus / Q000032:analysis* / Q000648:ultrastructure
chemical_list: D001426:Bacterial Proteins; D008565:Membrane Proteins