Preliminary studies on the cultivation and characterization of mini-pig prostate epithelial cells.
Mini-pig prostate epithelial cells exhibited the unique metabolic characteristics associated with the specialized function of production and secretion of high levels of citric acid. Epithelial cell suspensions from mini-pig prostate were successfully grown in primary and secondary cultures. The cultured epithelial cells exhibited rapid proliferation reaching confluency in approximately 6 days. Growth and proliferation of fibroblasts were markedly restricted by the dominance of epithelial cell growth. Confluent cultures could be maintained for approximately 6 weeks. The epithelial cells retained their polymorphic appearance in primary and secondary cultures and exhibited the characteristic formalin-resistant acid phosphatase reaction. Testosterone stimulated mitochondrial aspartate aminotransferase (mAAT) activity and citrate production by confluent epithelial cell cultures. These initial results indicate that cultured epithelial cells derived from mini-pig prostate might be an excellent model related to human for studies of prostate biology and hormonal regulation.
Costello
L C
LC
Department of Physiology, Dental School University of Maryland, Baltimore 21201.
Khan
M A
MA
Franklin
R B
RB
D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism; D002455:Cell Division; D002478:Cells, Cultured; D002951:Citrates / Q000378:metabolism; D019343:Citric Acid; D008297:Male; D011467:Prostate / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D013552:Swine; D013556:Swine, Miniature / Q000502:physiology*; D013739:Testosterone / Q000494:pharmacology
D002951:Citrates; D019343:Citric Acid; D013739:Testosterone; D001219:Aspartate Aminotransferases