C-PON containing neurons in the rat striatum are also positive for NADPH-diaphorase activity. A light microscopic study.
The indirect immunofluorescence technique to detect endogenous C-flanking peptide of neuropeptide Y (NPY), C-PON, in combination with NADPH-diaphorase (NADPH-d) histochemistry, were applied to the same sections to establish whether C-PON containing cell bodies in the rat striatum can be labelled by their content of NADPH-diaphorase activity. NADPH-diaphorase activity proved to be a reliable marker for these positive C-PON neurons. Our results suggest that, in the rat striatum, C-PON, SOM and NPY co-exist in the same cells.
Villalba
R M
RM
Unidad de Neuroanatomía, Instituto Cajal, C.S.I.C., Madrid, Spain.
Martínez-Murillo
R
R
Blasco
I
I
Alvarez
F J
FJ
Rodrigo
J
J
D000818:Animals; D003342:Corpus Striatum / Q000032:analysis*; D006651:Histocytochemistry; D007150:Immunohistochemistry; D008297:Male; D009247:NADH, NADPH Oxidoreductases / Q000032:analysis*; D009252:NADPH Dehydrogenase / Q000032:analysis*; D009478:Neuropeptide Y / Q000032:analysis*; D010446:Peptide Fragments / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains
D009478:Neuropeptide Y; D010446:Peptide Fragments; C048612:neuropeptide Y C-terminal flanking peptide; D009247:NADH, NADPH Oxidoreductases; D009252:NADPH Dehydrogenase