D-penicillamine reverses the inhibition of proteoglycan biosynthesis caused by exposure of cultured articular cartilage to hydrogen peroxide.
The sulphydryl containing anti-rheumatic drug D-penicillamine mildly inhibited proteoglycan synthesis in cartilage explant cultures by a mechanism not dependent upon H2O2 generation. More importantly, this drug alleviated the suppression of PG synthesis mediated by 10(-4) M H2O2 in a dose-dependent manner at concentrations of reduced drug similar to those plasma levels reported in vivo. The ability of D-penicillamine to reverse this effect was due solely to a reaction which resulted in scavenging of medium H2O2 and was not due to the "repair" of cellular lesions caused by prior exposure to H2O2.
Baker
M S
MS
Dept. of Biochemistry, Monash University, Clayton, Victoria, Australia.
Feigan
J
J
Lowther
D A
DA
D000818:Animals; D002358:Cartilage, Articular / Q000378:metabolism*; D002415:Cats; D046508:Culture Techniques; D004305:Dose-Response Relationship, Drug; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D010396:Penicillamine / Q000494:pharmacology*; D011509:Proteoglycans / Q000096:biosynthesis*
D011509:Proteoglycans; D006861:Hydrogen Peroxide; D010396:Penicillamine