The utilization of monoclonal antibodies in the diagnosis of unusual leukemias.
Development of monoclonal antibodies to lymphoid cells has allowed for simple methods for the classification of leukemias. Using the monoclonal antibodies, B1, B4, T1, T11, Ia, CALLA, My7, My9, and a polyclonal TdT reagent, we report a number of unusual phenotypes analyzed by flow cytometry. Two cases of mixed linkage leukemia are reported, one marked with anti-CALLA and My9, the other marked with T11 and Ia. Three rare cases of pediatric CLL are reported. Both were of B cell origin with chromosome transformation. These studies demonstrate the clinical importance of monoclonal antibodies in leukemia classification. The results also show that the so-called rare leukemias may not be as rare as previously thought.
Measel
J W
JW
Department of Microbiology and Immunology, Texas College of Osteopathic Medicine, Fort Worth 76107.
Ferguson
K A
KA
Distefano
A
A
D000293:Adolescent; D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor / Q000032:analysis; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D007938:Leukemia / Q000145:classification / Q000175:diagnosis*; D008297:Male; D010641:Phenotype
D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor