Application of Y chromosomal repetitive sequences to sexing mouse embryos.
Identification of sex is often necessary to evaluate genetic or teratogenetic effects on embryonic development. A simple molecular technique to identify the sex of mouse embryos was studied using a Y chromosomal repetitive sequence (designated 145SC5). Since this technique does not require purification of DNA, it is particularly suitable for processing many embryos. Furthermore, 145SC5 detects 1% contamination of male DNA in a male-female DNA mixture. These results suggest that 145SC5 is a powerful molecular tool in a variety of studies on mouse development.
Nishioka
Y
Y
Department of Biology, McGill University, Montreal, Quebec, Canada.
D000818:Animals; D015139:Blotting, Southern; D004247:DNA / Q000235:genetics; D004622:Embryo, Mammalian; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009693:Nucleic Acid Hybridization; D012091:Repetitive Sequences, Nucleic Acid; D012732:Sex Determination Analysis; D014998:Y Chromosome
D004247:DNA