Neutrophil retention in model capillaries: deformability, geometry, and hydrodynamic forces.
The initial retention of neutrophils within the pulmonary microvascular bed occurs in both physiological and pathological states, yet the factors responsible for this retention are poorly understood. Because the diameter of the neutrophil is approximately 7.03 micron and the mean pulmonary capillary diameter is 5.5 micron, we postulated that geometric constraints imposed by the microvascular bed, the deformability of the neutrophil, and the hydrodynamic characteristics of blood were important determinants of neutrophil retention. We used a filtration system wherein 111In-labeled human neutrophils (111In-N) suspended in a serum-containing buffer were passed through Nuclepore filters of known pore size. Compared with 99mTc-labeled erythrocytes (99mTc-RBC), the passage of 111In-N was delayed and a higher percentage was retained within the filter. Because the neutrophil and RBC are approximately equal in diameter, the deformability of the neutrophil must be less than that of RBC. As the flow rate increased, retention in the filters decreased logarithmically from 72 +/- 5% (flow rate 0.5 ml/min) to 15 +/- 4% (10.0 ml/min). As the number of RBC in the buffer increased, neutrophil retention in 5-micron filters decreased in a linear fashion from 65 +/- 6% at hematocrit of 0 to 33 +/- 2% at hematocrit of 10. The perfusion pressure and shear stress were of critical importance, and there was a logarithmic relationship between retention and perfusion pressure or shear stress (tau), whether the increase in pressure or tau was generated by increasing flow or by increasing the hematocrit of the perfusate. As the pore size of the filter increased, the retention of neutrophils decreased in a logarithmic fashion: from 75 +/- 5% in the 3-micron filter to 4 +/- 1.3% in the 12-micron filter.(ABSTRACT TRUNCATED AT 250 WORDS)
Downey
G P
GP
Department of Medicine, National Jewish Center for Immunology and Respiratory Medicine, Denver, Colorado.
Worthen
G S
GS
D055592:Biophysical Phenomena; D001703:Biophysics; D002196:Capillaries / Q000166:cytology; D004907:Erythrocyte Deformability; D004912:Erythrocytes / Q000166:cytology; D006801:Humans; D007205:Indium Radioisotopes; D007700:Kinetics; D007958:Leukocyte Count; D008168:Lung / Q000098:blood supply*; D008851:Micropore Filters; D008854:Microscopy, Electron; D009504:Neutrophils / Q000166:cytology / Q000502:physiology*; D010477:Perfusion; D011312:Pressure; D013696:Temperature
D007205:Indium Radioisotopes