[Design of a recombinant strain of the vaccinia virus containing an expressible gene for influenza A virus hemagglutinin].
A recombinant vaccinia virus (VV) strain containing a cloned gene of influenza A/Udorn/307/72 (H3N2) hemagglutinin (HA) gene has been produced. HA expression in CV-1 cells infected with the recombinant virus was determined by enzyme immunoassay. The influenza virus HA titer was 1:64-1:128. When rabbits were inoculated intravenously with the recombinant VaV, antibody titres were 1:5120. The recombinant VaV preparation may be used for generation of monospecific antibody to influenza virus.
Gibadulin
R A
RA
Lazarenko
A A
AA
Iuferov
V P
VP
Lideman
L F
LF
Denisova
G F
GF
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D004274:DNA, Recombinant; D004279:DNA, Viral / Q000235:genetics*; D005786:Gene Expression Regulation; D005814:Genes, Viral; D006389:Hemagglutinins, Viral / Q000235:genetics* / Q000276:immunology; D007114:Immunization; D009980:Influenza A virus / Q000235:genetics* / Q000276:immunology; D010957:Plasmids; D011817:Rabbits; D014162:Transfection; D014616:Vaccinia virus / Q000235:genetics* / Q000276:immunology; D014776:Virus Cultivation
D000914:Antibodies, Viral; D004274:DNA, Recombinant; D004279:DNA, Viral; D006389:Hemagglutinins, Viral
Konstruirovanie rekombinantnogo shtamma virusa ospovaktsiny, soderzhashchego ékspressiruemyĭ gen gemaggliutinina virusa grippa A.