Ex-vivo perfusion of surgically removed organs.
Surgically removed organs were perfused with oxygenated perfluorochemical blood substitute(FDA-O2). The perfusion was carried out using resected mouse liver. ATP level of perfused liver was remaining normal until 80 min. ATP peak disappeared when perfusion was discontinued, and it reappeared on reperfusion. No significant ischemic changes were demonstrated. Ex-vivo autoradiogram successfully labelled the S phase cells in various organs and various diseases. The absorptive study is also performed. Ex-vivo organ perfusion with FDA-O2 is able to keep the resected human organs viable, and is valuable new method to analyze the function and histogenesis of pathology.
Baba
S
S
2nd Department of Surgery, Hamamatsu University School of Med., Japan.
Nakai
K
K
Mizutani
K
K
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001802:Blood Substitutes; D004338:Drug Combinations; D005466:Fluorocarbons; D006895:Hydroxyethyl Starch Derivatives; D007408:Intestinal Absorption; D007422:Intestines / Q000098:blood supply / Q000502:physiology*; D008099:Liver / Q000098:blood supply / Q000502:physiology*; D051379:Mice; D010100:Oxygen / Q000097:blood; D010477:Perfusion
D001802:Blood Substitutes; D004338:Drug Combinations; D005466:Fluorocarbons; D006895:Hydroxyethyl Starch Derivatives; C008807:perfluorotributylamine; C017323:glucose, glycerol, hydroxyethyl starch, perfluorodecalin, perfluorotripropylamine, pluronic F-68, salts, yolk phospholipids drug combination; D000255:Adenosine Triphosphate; D010100:Oxygen