title: Sedimentation coefficient and minimum molecular weight of extracellular hemoglobin of Glossoscolex paulistus (Oligochaeta).
abstract: A new procedure for the determination of the sedimentation coefficient in the 50-1000 S range was designed and tested. The characteristics of this protocol are: the use of density gradients self-generated by osmosedimentation and the use of a low-speed centrifuge and of visual monitoring of the sedimenting zone. This procedure was used to determine the sedimentation coefficient of erythrocruorin from Glossoscolex paulistus. The value obtained, S20, omega = 58 S, corresponds to a MW of 3.1 x 10(6) Daltons. The minimum MW (heme), determined by the pyridine-hemochromogen method, was 25,250 Da.
authors.affiliation: Departamento de Química, Universidade Federal do Maranhão, São Luís, Brasil.
authors.forename: M C
authors.initials: MC
authors.lastname: Costa
authors.forename: C F
authors.initials: CF
authors.lastname: Bonafé
authors.forename: N C
authors.initials: NC
authors.lastname: Meirelles
authors.forename: F
authors.initials: F
authors.lastname: Galembeck
mesh_terms: D000818:Animals; D002499:Centrifugation, Density Gradient; D004902:Erythrocruorins / Q000032:analysis*; D006454:Hemoglobins / Q000032:analysis*; D008970:Molecular Weight; D009835:Oligochaeta
chemical_list: D004902:Erythrocruorins; D006454:Hemoglobins