The effects of environmental stress on steroid receptor levels and steroid-induced morphogenesis in Achlya ambisexualis.
Incubation of Achlya ambisexualis at elevated temperature (heat shock) or in the presence of sodium arsenite resulted in an inhibition of steroid hormone-induced responsiveness. The effect of heat shock was time- and temperature-dependent and more severe than the effect of sodium arsenite. Incubation at 37 degrees C for 30 min completely abolished the steroid-induced response and full recovery was not observed until 6 h after a return to the normal growth temperature of 22 degrees C. Heat shock and arsenite treatment had no effect on the cellular uptake of the steroid hormone, but heat shock resulted in a time- and temperature-dependent loss in the cellular level of steroid receptors. In contrast, arsenite treatment had little effect on the concentration of steroid receptors. However, both heat shock and arsenite treatment produced a long-term (4 h) and transient (1 h) inhibition of total protein synthesis, respectively. The recovery of steroid-induced responsiveness following heat shock was observed after both protein synthesis and steroid hormone receptor levels had returned to normal values.
Riehl
R M
RM
Department of Obstetrics and Gynecology, University of Texas Health Science Center, San Antonio 78284.
D005658:Fungi; D006358:Hot Temperature; D009024:Morphogenesis; D011987:Receptors, Steroid / Q000378:metabolism*
D011987:Receptors, Steroid