Studies on the stability of muroctasin and degradation products built under extreme conditions.
Decomposition of N2-[(N-acetylmuramoyl)-L-alanyl-D-isoglutaminyl]-N6-stearoyl-L-lysine (MDP-Lys(L18), muroctasin) under extreme conditions was investigated. MDP-Lys(L18) in an aqueous solution was heated under reflux for 4 h to give the decomposition products D-1, D-2, D-3 and D-4. Reaction of MDP-Lys(L18) with 0.1N NaOH at room temperature for 5 h gave D-1, and that with 1 N HCl under reflux for 1 h afforded D-4. MDP-Lys(L18) was found to be stable to light and heat. MDP-Lys(L18) in a powder form was stored at room temperature or at 25 degrees C and 75% relative humidity for 3 days to 18 months. Quantitative analysis, thin-layer chromatography, and tests for appearance and color change, etc., were performed in the stored samples. MDP-Lys(L18) was found to be stable, but hygroscopic.
Moroi
R
R
Research Institute, Daiichi Seiyaku Co., Ltd., Tokyo, Japan.
Yamazaki
K
K
Hirota
T
T
Watanabe
S
S
Oki
M
M
Toriyama
M
M
Ichinohe
A
A
Kataoka
K
K
D000119:Acetylmuramyl-Alanyl-Isoglutamine / Q000031:analogs & derivatives* / Q000378:metabolism; D000276:Adjuvants, Immunologic / Q000378:metabolism*; D000596:Amino Acids / Q000032:analysis; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D004355:Drug Stability; D006358:Hot Temperature; D006813:Humidity; D006863:Hydrogen-Ion Concentration; D008027:Light; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D011208:Powders; D012015:Reference Standards; D013055:Spectrophotometry, Infrared; D013997:Time Factors
D000276:Adjuvants, Immunologic; D000596:Amino Acids; D011208:Powders; D000119:Acetylmuramyl-Alanyl-Isoglutamine; C036866:romurtide