title: Human progesterone A-receptors can be synthesized intracellularly and are biologically functional.
abstract: In order to investigate the origin and functional independence of the human progesterone receptor A binding protein, we have expressed a truncated human progesterone receptor cDNA in both gene transfer and in vitro translation assays. Proteins identical in size and antigenicity to the A-receptors found naturally in human progesterone target cells are synthesized from this cDNA that lacks the putative B receptor initiator methionine codon of the complete cDNA. The functional independence of A-receptors is suggested by their ability to bind hormone and to stimulate transcription from the progestin responsive mouse mammary tumor virus promoter.
authors.affiliation: Department of Medicine, University of Colorado Health Sciences Center, Denver 80262.
authors.forename: N L
authors.initials: NL
authors.lastname: Krett
authors.forename: L L
authors.initials: LL
authors.lastname: Wei
authors.forename: M D
authors.initials: MD
authors.lastname: Francis
authors.forename: S K
authors.initials: SK
authors.lastname: Nordeen
authors.forename: D F
authors.initials: DF
authors.lastname: Gordon
authors.forename: W M
authors.initials: WM
authors.lastname: Wood
authors.forename: K B
authors.initials: KB
authors.lastname: Horwitz
mesh_terms: D015153:Blotting, Western; D003001:Cloning, Molecular; D003593:Cytoplasm / Q000378:metabolism; D004247:DNA / Q000235:genetics; D005786:Gene Expression Regulation; D006801:Humans; D011233:Precipitin Tests; D011397:Promegestone / Q000378:metabolism; D011980:Receptors, Progesterone / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D014162:Transfection
chemical_list: D011980:Receptors, Progesterone; D004247:DNA; D011397:Promegestone