title: Fractionation of Bothrops jararacussu snake venom: partial chemical characterization and biological activity of bothropstoxin.
abstract: A myotoxin, bothropstoxin (BthTX), showing no detectable phospholipase A2 activity, was purified to homogeneity from the venom of the Brazilian snake Bothrops jararacussu by a combination of gel filtration on Sephadex G-75 and ion-exchange chromatography on SP-Sephadex C-25. Four phospholipases (Sm-SP1 to Sm-SPIV) were also isolated, the latter showing, similarly to BthTX (Sm-SPv) myonecrotic activity. Approximate mol. wts, as determined by SDS-PAGE, and pI of Sm-SPI to Sm-SPIV are: 22,400-4.2; 15,500-4.8; 13,800-6.9; and 13,200-7.7, respectively. BthTX is a single chain protein, approximate mol. wt 13,000, with 16 half-cystine residues, pI = 8.2 and LD50 = 7.5 mg/kg (i.p.) and 4.8 mg/kg (i.v.) for 20 g mice. The ten first N-terminal amino acid residues show a significant homology to other toxins with phospholipase structure. BthTX is specifically myotoxic, contrary to crude B. jararacussu venom which, although also myotoxic, affects intramuscular arteries and veins leading to thrombosis. BthTX and Sm-SPIV also differ from toxins isolated from the venom of other Brazilian snakes which are strongly hemorrhagic.
authors.affiliation: Departamento de Bioquímica, Faculdade de Medicina, Universidade de São Paulo, Brazil.
authors.forename: M I
authors.initials: MI
authors.lastname: Homsi-Brandeburgo
authors.forename: L S
authors.initials: LS
authors.lastname: Queiroz
authors.forename: H
authors.initials: H
authors.lastname: Santo-Neto
authors.forename: L
authors.initials: L
authors.lastname: Rodrigues-Simioni
authors.forename: J R
authors.initials: JR
authors.lastname: Giglio
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002850:Chromatography, Gel; D003435:Crotalid Venoms / Q000032:analysis* / Q000633:toxicity; D051379:Mice; D008969:Molecular Sequence Data; D009132:Muscles / Q000187:drug effects*; D010741:Phospholipases A / Q000032:analysis; D054467:Phospholipases A2
chemical_list: D000596:Amino Acids; D003435:Crotalid Venoms; D010741:Phospholipases A; D054467:Phospholipases A2