title: Microassay for adenosine deaminase, the enzyme lacking in some forms of immunodeficiency, in mouse preimplantation embryos.
abstract: A highly sensitive biochemical microassay has been developed for adenosine deaminase (ADA; EC 3.5.4.4), the enzyme deficient in approximately 20% of cases of severe combined immuno-deficiency disease (SCID). The microassay is capable of detecting femtomolar amounts of reaction product in a single blastomere from a mouse 8-cell embryo and thus is sensitive enough to be considered for the possible preimplantation diagnosis of SCID in human embryos.
authors.affiliation: MRC Mammalian Development, University College London, UK.
authors.forename: C
authors.initials: C
authors.lastname: Benson
authors.forename: M
authors.initials: M
authors.lastname: Monk
mesh_terms: D000243:Adenosine Deaminase / Q000032:analysis* / Q000172:deficiency; D000818:Animals; D001755:Blastocyst / Q000201:enzymology*; D005260:Female; D007154:Immune System Diseases / Q000175:diagnosis / Q000201:enzymology*; D051379:Mice; D009700:Nucleoside Deaminases / Q000032:analysis*; D011247:Pregnancy; D011296:Prenatal Diagnosis
chemical_list: D009700:Nucleoside Deaminases; D000243:Adenosine Deaminase