title: Production, purification and molecular weight determination of the haemolysin of Treponema hyodysenteriae.
abstract: The production of haemolysin from Treponema hyodysenteriae was increased by an improved culture method and by repeated incubation of spirochaetes suspended in a buffer containing RNA-core. Ion exchange chromatography on DEAE cellulose followed by gel filtration on Sephadex G100 yielded purified haemolysin free from extraneous protein, as judged by silver-stained polyacrylamide gels. The mol. wt of the purified haemolysin, determined by gel filtration was 19,000, a value similar to that of streptolysin S, but much lower than that previously reported.
authors.affiliation: A.F.R.C. Institute for Animal Health, Compton Laboratory, Newbury, Berkshire.
authors.forename: K A
authors.initials: KA
authors.lastname: Kent
authors.forename: R M
authors.initials: RM
authors.lastname: Lemcke
authors.forename: R J
authors.initials: RJ
authors.lastname: Lysons
mesh_terms: D000818:Animals; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006460:Hemolysin Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D014208:Treponema / Q000254:growth & development / Q000378:metabolism*; D014462:Ultrafiltration
chemical_list: D006460:Hemolysin Proteins