title: Experimental osteoarthritic articular cartilage is enriched in guanidine soluble type VI collagen.
abstract: Experimental osteoarthritis was surgically induced in the right knee joint of dogs; the left knee served as a control. Articular cartilage was extracted with 4 M guanidinium chloride, 0.05 M sodium acetate, pH 6.0, containing proteinase inhibitors and the proteins purified by associative CsCl density gradient centrifugation. Equal quantities of protein were electrophoresed in agarose-acrylamide gradient gels and the high molecular weight type VI collagen bands detected in immunoblots with a polyclonal antiserum. Type VI collagen bands between 185 and 220 kDa were evident in the pathological specimens of dogs sacrificed 3, 5, and 7 months after surgery and were either absent or only very weakly visible in the controls. These results demonstrate that experimental osteoarthritic cartilage is enriched in 4 M guanidine-soluble type VI collagen.
authors.affiliation: Department of Musculoskeletal Research, Cleveland Clinic Foundation Research Institute, Ohio 44195.
authors.forename: C A
authors.initials: CA
authors.lastname: McDevitt
authors.forename: J A
authors.initials: JA
authors.lastname: Pahl
authors.forename: S
authors.initials: S
authors.lastname: Ayad
authors.forename: R R
authors.initials: RR
authors.lastname: Miller
authors.forename: M
authors.initials: M
authors.lastname: Uratsuji
authors.forename: J T
authors.initials: JT
authors.lastname: Andrish
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002358:Cartilage, Articular / Q000378:metabolism*; D003094:Collagen / Q000378:metabolism*; D004285:Dogs; D006146:Guanidines; D008970:Molecular Weight; D010003:Osteoarthritis / Q000378:metabolism*; D012995:Solubility
chemical_list: D006146:Guanidines; D003094:Collagen