Isolation of a hemolysin from a spore-crystal mixture of Bacillus thuringiensis israelensis (serotype H-14).
A hemolytic toxin from Bacillus thuringiensis israelensis was obtained by alkaline extraction and fast protein liquid chromatography (chromatofocusing followed by gel filtration). The toxin displayed a pI of 4.6-4.8 and an Mr of 26,000. Amino acid analysis demonstrated large amounts of serine, glycine and glutamic acid. The toxin was strongly lytic for rabbit, human and non-human primate erythrocytes, and was weakly lytic toward equine, feline, canine, bovine, amphibian and reptilian erythrocytes. It was weakly entomocidal as indicated by a lethal potency (LC50) of 25 micrograms/ml for second instar larvae of Anopheles stephansi. Direct micro-ELISA indicated that the toxin lacked antigenic identity with numerous eukaryotic and prokaryotic toxins and venoms.
Weinstein
S A
SA
Department of Microbiology, New York University School of Medicine, NY 10016.
Bernheimer
A W
AW
Oppenheim
J D
JD
D000596:Amino Acids / Q000032:analysis; D000663:Amphibians; D000818:Animals; D001413:Bacillus thuringiensis; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins / Q000302:isolation & purification*; D002415:Cats; D002417:Cattle; D004285:Dogs; D004591:Electrophoresis, Polyacrylamide Gel; D004731:Endotoxins; D004912:Erythrocytes / Q000187:drug effects; D006460:Hemolysin Proteins / Q000302:isolation & purification*; D006801:Humans; D007123:Immunoelectrophoresis, Two-Dimensional; D012104:Reptiles; D012756:Sheep; D013045:Species Specificity
D000596:Amino Acids; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004731:Endotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis