ATP stimulates the uptake of S-dinitrophenylglutathione by rat liver plasma membrane vesicles.
Incubation of inverted plasma membrane vesicles from rat liver with micromolar concentrations of S-dinitrophenylglutathione (DNP-SG) in the presence of ATP resulted in the uptake of DNP-SG into the vesicles. ATP-dependent DNP-SG accumulation was half-maximal with 9 microM DNP-SG, while the Km for ATP was 320 microM. Glutathione disulfide (GSSG), but not reduced glutathione, inhibited the ATP-dependent accumulation of DNP-SG by the vesicles, suggesting that the same, ATP-dependent transport system is responsible for the extrusion of glutathione conjugates and GSSG from liver cells.
Kobayashi
K
K
Institute of Scientific and Industrial Research, Osaka University, Ibaraki, Japan.
Sogame
Y
Y
Hayashi
K
K
Nicotera
P
P
Orrenius
S
S
D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D005978:Glutathione / Q000031:analogs & derivatives* / Q000378:metabolism*; D066298:In Vitro Techniques; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D008297:Male; D009994:Osmolar Concentration; D051381:Rats; D011919:Rats, Inbred Strains
C030190:S-(2,4-dinitrophenyl)glutathione; D000255:Adenosine Triphosphate; D005978:Glutathione