High-performance liquid chromatographic determination of trans-doxepin and desmethyldoxepin.
An improved high performance liquid chromatographic (HPLC) assay for the quantitative determination of trans-doxepin (I) and desmethyldoxepin (II) in body fluids is presented. This HPLC assay, employing a UV-detector and perazine (III) as an internal standard, provides a very sensitive and selective determination in the low ng/ml range. The lower limit of quantification was 0.426 ng/ml (I) and 0.50 ng/ml (II); respectively. The calibration curve was linear in the measured range of 0.426-34.08 ng/ml (I) and 0.50-40 ng/ml (II). In combination with the excellent precision and accuracy data (c.v. values typically lower than 5%) and a recovery exceeding 90% for both compounds, the method is well suited for quantitative determinations of plasma samples generated during clinical studies, eg. evaluating the pharmacokinetics and/or bioavailability/bioequivalence as well as evaluations of clinical response.
Dilger
C
C
Analytical Research Centre, LAB GmbH, Neu-Ulm, Fed. Rep. of Germany.
Salama
Z
Z
Jaeger
H
H
D000929:Antidepressive Agents, Tricyclic / Q000097:blood*; D002138:Calibration; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D004316:Doxepin / Q000031:analogs & derivatives* / Q000097:blood*; D006801:Humans; D007536:Isomerism; D010464:Perazine; D011237:Predictive Value of Tests; D011786:Quality Control; D012015:Reference Standards
D000929:Antidepressive Agents, Tricyclic; D004316:Doxepin; D010464:Perazine; C028701:desmethyldoxepin