title: [Synthesis of new terminator substrates for DNA-polymerases-- nucleoside-5'-triphosphates with modified carbohydrate residue].
abstract: Synthesis of 3'-chloro- and 3'-cyanothio-2',3'-dideoxythymidine by the reaction of 2,3'-anhydro-2'-deoxythymidine with ammonium chloride and lithium thiocyanate, respectively, has been developed. In addition, 3'-methanesulphonylamido- and 3'-sulphonylamido-2',3'-dideoxythymidines were synthesized starting from 3'-amino-2',3'-dideoxythymidine. All these compounds along with 2',3'-anhydroriboadenosine,2',3'-anhydrolyxoadenosine, 2',3'-O-isopropylidenecytidine, and 2,3'-anhydro-2'-deoxythymidine were transformed into 5'-triphosphates by treatment with phosphoryl tris-1,2,4-triazolide and then with bis(tri-n-butylammonium)pyrophosphate. All 5'-triphosphates of nucleoside analogues were tested as termination substrates in cell-free systems with various DNA polymerases.
authors.forename: N B
authors.initials: NB
authors.lastname: Diatkina
authors.forename: E D
authors.initials: ED
authors.lastname: Atrazheva
authors.forename: L A
authors.initials: LA
authors.lastname: Aleksandrova
authors.forename: A A
authors.initials: AA
authors.lastname: Kraevskiĭ
authors.forename: M
authors.initials: M
authors.lastname: von Janta-Lipinski
mesh_terms: D000818:Animals; D002241:Carbohydrates; D002384:Catalysis; D002417:Cattle; D002474:Cell-Free System; D055598:Chemical Phenomena; D002621:Chemistry; D004259:DNA-Directed DNA Polymerase; D009711:Nucleotides / Q000138:chemical synthesis*; D013379:Substrate Specificity; D013950:Thymus Gland / Q000201:enzymology
chemical_list: D002241:Carbohydrates; D009711:Nucleotides; D004259:DNA-Directed DNA Polymerase
vernacular_title: Sintez novykh terminatornykh substratov DNK-polimeraz-- nukleozid-5'-trifosfatov s modifitsirovannym uglevodnym ostatkom.