title: Duck lens epsilon-crystallin and lactate dehydrogenase B4 are identical: a single-copy gene product with two distinct functions.
abstract: To investigate whether or not duck lens epsilon-crystallin and duck heart lactate dehydrogenase (LDH) B4 are the product of the same gene, we have isolated and sequenced cDNA clones of duck epsilon-crystallin. By using these clones we demonstrate that there is a single-copy Ldh-B gene in duck and in chicken. In the duck lens this gene is overexpressed, and its product is subject to posttranslational modification. Reconstruction of the evolutionary history of the LDH protein family reveals that the mammalian Ldh-C gene most probably originated from an ancestral Ldh-A gene and that the amino acid replacement rate in LDH-C is approximately 4 times the rate in LDH-A. Molecular modeling of LDH-B sequences shows that the increased thermostability of the avian tetramer might be explained by mutations that increase the number of ion pairs. Furthermore, the replacement of bulky side chains by glycines on the corners of the duck protein suggests an adaptation to facilitate close packing in the lens.
authors.affiliation: Department of Biochemistry, University of Nijmegen, The Netherlands.
authors.forename: W
authors.initials: W
authors.lastname: Hendriks
authors.forename: J W
authors.initials: JW
authors.lastname: Mulders
authors.forename: M A
authors.initials: MA
authors.lastname: Bibby
authors.forename: C
authors.initials: C
authors.lastname: Slingsby
authors.forename: H
authors.initials: H
authors.lastname: Bloemendal
authors.forename: W W
authors.initials: WW
authors.lastname: de Jong
mesh_terms: D000818:Animals; D001483:Base Sequence; D002645:Chickens; D003459:Crystallins / Q000235:genetics*; D004247:DNA / Q000032:analysis; D004372:Ducks; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny
chemical_list: D003459:Crystallins; D007527:Isoenzymes; D004247:DNA; D007770:L-Lactate Dehydrogenase