Complete amino acid sequence of human placental protein 14: a progesterone-regulated uterine protein homologous to beta-lactoglobulins.
Placental protein 14 (PP14), also known as progestagen-dependent endometrial protein and pregnancy-associated endometrial alpha 2-globulin, is synthesized by the human secretory endometrium and decidua. We have isolated from a human decidual cDNA library clones corresponding to PP14 and deduced its entire amino acid sequence. PP14 contains 180 amino acids, 18 of which correspond to a putative signal peptide. The predicted molecular weight of the pre-PP14 is 20,555 and that of the mature protein is 18,787. PP14 is encoded by a 1-kilobase-pair mRNA that is expressed in human secretory endometrium and decidua but not in postmenopausal endometrium, placenta, liver, kidney, and adrenals. The 162-residue-long sequence of PP14 is highly homologous to beta-lactoglobulins, with a 53.4% identity with the amino acid sequence of horse beta-lactoglobulin I. The four cysteinyl residues (positions 66, 106, 119, and 160) responsible for intramolecular disulfide bridges in beta-lactoglobulins are all conserved in PP14. Southern blot analysis of human DNA suggested that PP14 gene sequences encompass some 20 kilobase pairs of the human genomic DNA.
Julkunen
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Rockefeller University, New York, NY 10021.
Seppälä
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Jänne
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D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D004247:DNA / Q000235:genetics; D003656:Decidua / Q000378:metabolism; D004717:Endometrium / Q000378:metabolism; D005260:Female; D005796:Genes; D000071816:Glycodelin; D006023:Glycoproteins; D006736:Horses; D006801:Humans; D007782:Lactoglobulins / Q000235:genetics*; D008969:Molecular Sequence Data; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000235:genetics* / Q000302:isolation & purification; D011261:Pregnancy Trimester, First; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid
D000071816:Glycodelin; D006023:Glycoproteins; D007782:Lactoglobulins; C045246:PAEP protein, human; D011257:Pregnancy Proteins; D012333:RNA, Messenger; D004247:DNA