Conversion of Woodward's Reagent K in an aqueous medium: mathematical analysis applied to enzyme modification.
A change in the optical density of Woodward's Reagent K solution at 340 nm has been shown. It is observed after the reagent has been dissolved in a weakly acidic medium. The optical density correlates with the reagent's ability to inhibit transketolase. A method for assay of the inhibitor concentration changes in the medium during enzyme modification is suggested.
Kuimov
A N
AN
A. N. Belozersky Laboratory of Molecular Biology and Bioorganic Chemistry, Moscow State University, USSR.
Kochetov
G A
GA
D007202:Indicators and Reagents / Q000032:analysis* / Q000494:pharmacology; D007555:Isoxazoles / Q000032:analysis* / Q000494:pharmacology; D007700:Kinetics; D008433:Mathematics; D008956:Models, Chemical; D009243:NAD / Q000032:analysis; D010080:Oxazoles / Q000032:analysis*; D010084:Oxidation-Reduction; D013056:Spectrophotometry, Ultraviolet; D014174:Transketolase / Q000037:antagonists & inhibitors*
D007202:Indicators and Reagents; D007555:Isoxazoles; D010080:Oxazoles; D009243:NAD; C009686:N-ethyl-5-phenylisoxazolium-3'-sulfonate; D014174:Transketolase