Amino acid sequences of two nonspecific lipid-transfer proteins from germinated castor bean.
The amino acid sequence of two nonspecific lipid-transfer proteins (nsLTP) B and C from germinated castor bean seeds have been determined. Both the proteins consist of 92 residues, as for nsLTP previously reported, and their calculated Mr values are 9847 and 9593 for nsLTP-B and nsLTP-C, respectively. The sequences of nsLTP-B and nsLTP-C, compared to the known sequence of nsLTP-A from the same source, are 68% and 35% similar, respectively. No variation was found at the positions of the cysteine residues, indicating that they might be involved in disulfide bridges.
Takishima
K
K
Department of Biochemistry, National Defense Medical College, Saitama, Japan.
Watanabe
S
S
Yamada
M
M
Suga
T
T
Mamiya
G
G
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D052179:Antigens, Plant; D002352:Carrier Proteins / Q000302:isolation & purification; D002367:Ricinus communis; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002918:Chymotrypsin; D004220:Disulfides / Q000378:metabolism; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000302:isolation & purification; D010940:Plant Proteins; D010947:Plants, Toxic; D012639:Seeds / Q000032:analysis*; D012697:Serine Endopeptidases; D014357:Trypsin
D000596:Amino Acids; D052179:Antigens, Plant; D002352:Carrier Proteins; D004220:Disulfides; D010446:Peptide Fragments; D010940:Plant Proteins; C511920:lipid transfer proteins, plant; D012697:Serine Endopeptidases; D002918:Chymotrypsin; C028344:glutamyl endopeptidase; D014357:Trypsin