Isolation and characterization of a bovine liver tyrosine-O-sulfate-binding protein--a putative receptor molecular for tyrosine-sulfated proteins?
Golgi-enriched microsomal membrane fraction was prepared from bovine liver. Sodium choleate extract of this membrane preparation was subjected to fractionation using Sepharose gel covalently bonded with tyrosine-O-sulfate. SDS gel electrophoresis of the fractionated sample revealed the presence of a major protein with an apparent molecular weight of 175,000. The protein appears to be specific for tyrosine-O-sulfate as it binds neither the unmodified tyrosine nor the structurally similar tyrosine-O-phosphate. pH-dependence study showed the binding of the protein to tyrosine-O-sulfate-Sepharose gel to be strong from pH 8.0 down through 6.0. At pH 5.5, the binding affinity became dramatically reduced. A similar tyrosine-O-sulfate-binding protein was also detected in the choleate extracts of the Golgi-enriched microsomal membrane fractions prepared from bovine pancreas and from both liver and pancreas of dog.
Liu
M C
MC
Department of Chemistry, University of Oklahoma, Norman 73019.
Suiko
M
M
Tang
X B
XB
D000818:Animals; D002352:Carrier Proteins / Q000302:isolation & purification* / Q000378:metabolism; D002417:Cattle; D019826:Cholic Acid; D002793:Cholic Acids; D002846:Chromatography, Affinity; D004285:Dogs; D004591:Electrophoresis, Polyacrylamide Gel; D006863:Hydrogen-Ion Concentration; D036341:Intercellular Signaling Peptides and Proteins; D007425:Intracellular Membranes / Q000032:analysis; D008862:Microsomes, Liver / Q000032:analysis*; D008970:Molecular Weight; D010179:Pancreas / Q000032:analysis; D014018:Tissue Distribution; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000378:metabolism
D002352:Carrier Proteins; D002793:Cholic Acids; D036341:Intercellular Signaling Peptides and Proteins; C057501:tyrosine-O-sulfate-binding protein, Bos taurus; C005662:tyrosine O-sulfate; D014443:Tyrosine; D019826:Cholic Acid