title: The terminal protein of minute virus of mice is an 83 kilodalton polypeptide linked to specific forms of double-stranded and single-stranded viral DNA.
abstract: A new assay (label transfer from DNA to protein) enabled the identification of a terminal protein (TP) in nucleoprotein complexes extracted from cells infected with the parvovirus, minute virus of mice, MVM. In SDS-PAGE, TP migrates as a major band at 83 kDa, with a minor 65 kDa component, each of which exactly co-migrates with the cellular forms of the virally coded polypeptide NS-1. In parallel, the analysis of nucleoproteins by SDS-agarose gel electrophoresis allowed us to observe that the major species of viral DNA molecules (mRF, dRF and ssDNA) are all present in the form of DNA-protein complexes. Three forms of mRF DNA were identified, two of which are protein-associated and one which appears to be protein-free.
authors.affiliation: Department of Laboratory Medicine, Yale School of Medicine, New Haven, CT 06510.
authors.forename: M
authors.initials: M
authors.lastname: Gunther
authors.forename: P
authors.initials: P
authors.lastname: Tattersall
mesh_terms: D000818:Animals; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D004277:DNA, Single-Stranded / Q000378:metabolism*; D004279:DNA, Viral / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D051379:Mice; D008915:Minute Virus of Mice / Q000378:metabolism*; D008970:Molecular Weight; D009698:Nucleoproteins / Q000032:analysis / Q000378:metabolism*; D010321:Parvoviridae / Q000378:metabolism*; D014764:Viral Proteins / Q000032:analysis / Q000378:metabolism*
chemical_list: D004277:DNA, Single-Stranded; D004279:DNA, Viral; D009698:Nucleoproteins; D014764:Viral Proteins