[Identification of 5-aminovaleric acid as a characteristic product of metabolism of various Clostridium species].
An unusual ninhydrin-positive compound has been isolated from feces of accidentally contaminated Sprague-Dawley autbred rats and identified by mass spectrometry and 1H-NMR spectroscopy techniques as 5-aminovaleric acid. The described procedure of isolation, purification and structure determination can be recommended as a general method of identification of unusual ninhydrin-positive compounds in complex mixtures of biological origin. The 5-aminovaleric acid was found to be produced by an anaerobic bacterium Clostridium bifermentans. It is shown that not all Clostridium spp. excrete his amino acid and that the majority of microorganisms, normally inhabiting intestine of rats, simians and man, do not possess this ability. On the basis of data obtained, the test for 5-aminovaleric acid is proposed to be included into the taxonomy of bacteria.
Rodionov
A V
AV
Trapezov
E V
EV
Dmitriev
B A
BA
Chakhava
O V
OV
D000596:Amino Acids / Q000302:isolation & purification* / Q000378:metabolism; D021542:Amino Acids, Neutral; D000818:Animals; D002852:Chromatography, Ion Exchange; D003013:Clostridium / Q000378:metabolism*; D005243:Feces / Q000382:microbiology; D013058:Mass Spectrometry; D051381:Rats; D011919:Rats, Inbred Strains; D013056:Spectrophotometry, Ultraviolet
D000596:Amino Acids; D021542:Amino Acids, Neutral; C013809:5-aminovaleric acid
Identifikatsiia 5-aminovalerianovoĭ kisloty kak kharakteristicheskogoprodukta metabolizma nekotorykh predstaviteleĭ roda klostridiĭ.