title: Isoprenoid biosynthesis in a marine sponge of the Amphimedon genus: incorporation studies with [1-14C] acetate, [4-14C] cholesterol and [2-14C] mevalonate.
abstract: 1. We present quantitative evidence from incorporation of [1-14C] acetate that the enzymes to synthesise isoprenoids are present in the marine sponge Amphimedon sp. and that efficient carotenoid synthesis takes place. 2. The de novo synthesis of b,b-carotene and (3R,3'R)-zeaxanthin may occur in a chlorophyll a-producing microalgal symbiont with subsequent aromatisation to (3R)-isoagelaxanthin by the sponge itself. 3. Amphimedon sp. contains nuclear-modified sterols derived by modification of conventional dietary sterols.
authors.affiliation: Department of Chemistry, University of Wollongong, NSW, Australia.
authors.forename: M J
authors.initials: MJ
authors.lastname: Garson
authors.forename: V
authors.initials: V
authors.lastname: Partali
authors.forename: S
authors.initials: S
authors.lastname: Liaaen-Jensen
authors.forename: I L
authors.initials: IL
authors.lastname: Stoilov
mesh_terms: D000085:Acetates / Q000378:metabolism; D019342:Acetic Acid; D000818:Animals; D002338:Carotenoids / Q000096:biosynthesis*; D002784:Cholesterol / Q000378:metabolism; D008798:Mevalonic Acid / Q000378:metabolism; D011161:Porifera / Q000378:metabolism*; D013261:Sterols / Q000096:biosynthesis; D013729:Terpenes / Q000096:biosynthesis* / Q000378:metabolism
chemical_list: D000085:Acetates; D013261:Sterols; D013729:Terpenes; D002338:Carotenoids; D002784:Cholesterol; D019342:Acetic Acid; D008798:Mevalonic Acid