title: The interaction of nuclear proteins with essential promoter element of the chicken cardiac myosin light chain 2 gene is involved in muscle-specific transcription.
abstract: A quantitative microinjection procedure has been developed to demonstrate muscle-specific transcription of the myosin light chain 2-A (MLC2-A) promoter in differentiated chicken primary breast muscle cells. Nuclear protein binds to the distal region of the required promoter sequence but not to a mutated version of this sequence. The functional significance of this specific DNA-protein interaction for the promoter activity is demonstrated by 'in vivo' competition of microinjected MLC-CAT reporter construct together with excess of synthetic oligonucleotides encompassing the protein binding sites.
authors.affiliation: Department of Toxicology, Medical School, University of Hamburg, FRG.
authors.forename: T
authors.initials: T
authors.lastname: Braun
authors.forename: G
authors.initials: G
authors.lastname: Buschhausen-Denker
authors.forename: E
authors.initials: E
authors.lastname: Tannich
authors.forename: H H
authors.initials: HH
authors.lastname: Arnold
mesh_terms: D000818:Animals; D001483:Base Sequence; D002645:Chickens; D005796:Genes; D008969:Molecular Sequence Data; D009132:Muscles / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism*; D015879:Myosin Subfragments; D009218:Myosins / Q000235:genetics*; D009687:Nuclear Proteins / Q000502:physiology*; D010446:Peptide Fragments / Q000235:genetics*; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D014158:Transcription, Genetic
chemical_list: D015879:Myosin Subfragments; D009687:Nuclear Proteins; D010446:Peptide Fragments; D009218:Myosins