Influence of flanking sequences on variability in expression levels of an introduced gene in transgenic tobacco plants.
The petunia rbcS gene SSU301 was introduced into tobacco using Agrobacterium tumefaciens-mediated transformation. The time at which rbcS expression was maximal after transfer of the tobacco plants to the greenhouse was determined. The expression level of the SSU301 gene varied up to 9 fold between individual tobacco plants which had been standardized physiologically as much as possible. The presence of adjacent pUC plasmid sequences did not affect the expression of the SSU301 gene. In an attempt to reduce the between-transformant variability in expression, the SSU301 gene was introduced into tobacco surrounded by 10kb of 5' and 13 kb of 3' DNA sequences which normally flank SSU301 in petunia. The longer flanking regions did not reduce the between-transformant variability of SSU301 gene expression.
Dean
C
C
Advanced Genetic Sciences Inc., Oakland, CA 94608.
Jones
J
J
Favreau
M
M
Dunsmuir
P
P
Bedbrook
J
J
D015152:Blotting, Northern; D004274:DNA, Recombinant; D005786:Gene Expression Regulation; D005822:Genetic Vectors; D010947:Plants, Toxic; D012333:RNA, Messenger / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid; D012273:Ribulose-Bisphosphate Carboxylase / Q000235:genetics; D014026:Nicotiana / Q000235:genetics*; D014170:Transformation, Genetic
D004274:DNA, Recombinant; D012333:RNA, Messenger; D012273:Ribulose-Bisphosphate Carboxylase