Effect of DIDS on osmotic properties of bovine erythrocytes.
The effect of DIDS on osmotic properties of bovine erythrocytes was studied. The isoosmotic volume, the amount of intracellular solutes, and the osmotically non-active volume were not influenced by DIDS. An increase of osmotic fragility of erythrocytes upon DIDS treatment was evident and identical both in NaCl and in NaCl + KCl hypotonic solutions. These results suggest that the critical cell volume decreases. The link between the effect of DIDS on the membrane skeleton extractability and on the osmotic fragility was postulated.
Mosior
M
M
Institute of Physics, Technical University of Wrocław, Poland.
Białas
W A
WA
Gomułkiewicz
J
J
D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid / Q000031:analogs & derivatives / Q000494:pharmacology*; D000818:Animals; D000838:Anions; D001639:Bicarbonates / Q000097:blood; D002417:Cattle; D002712:Chlorides / Q000097:blood; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism*; D006461:Hemolysis / Q000187:drug effects; D066298:In Vitro Techniques; D007700:Kinetics; D009994:Osmolar Concentration; D009996:Osmotic Fragility / Q000187:drug effects; D013267:Stilbenes / Q000494:pharmacology*
D000838:Anions; D001639:Bicarbonates; D002712:Chlorides; D013267:Stilbenes; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid