Mitogenic effect of urokinase on malignant and unaffected adjacent human renal cells.
Primary cultures of renal cell carcinomas and of the corresponding normal adjacent kidney tissue from 6 patients were analyzed for the effects of exogenously added urokinase-type plasminogen activator on cell proliferation as compared to the effects of tissue type plasminogen activator, plasmin and dihydrocortisone. Cell proliferation was studied over a period of up to 5 days by measuring 3H-thymidine incorporation as well as cell viability and cell count; conditioned media of the cultures were also analyzed for their plasminogen activator and plasminogen activator inhibitor content. Addition of urokinase stimulated cell proliferation in a time and dose dependent fashion; after 3 days 3H-thymidine incorporation was significantly increased in malignant renal cells (188.3 +/- 28.7%), while it reached in normal renal cells approximately 130% of the 3H-thymidine incorporation of untreated cultures. Tissue-type plasminogen activator had no effect and plasmin decreased cell proliferation slightly while dihydrocortisone inhibited cell proliferation significantly (34.1 +/- 4.9%) in malignant cells. It is concluded that urokinase-type plasminogen activator itself exhibits a mitogenic effect also on primary cultures of renal cell carcinomas.
Kirchheimer
J C
JC
Department of Medical Physiology, University of Vienna, Austria.
Wojta
J
J
Christ
G
G
Hienert
G
G
Binder
B R
BR
D000328:Adult; D000368:Aged; D002292:Carcinoma, Renal Cell / Q000473:pathology*; D004247:DNA / Q000096:biosynthesis; D005260:Female; D006801:Humans; D007668:Kidney / Q000166:cytology*; D007680:Kidney Neoplasms / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D008934:Mitogens; D014568:Urokinase-Type Plasminogen Activator / Q000494:pharmacology*
D008934:Mitogens; D004247:DNA; D014568:Urokinase-Type Plasminogen Activator