title: Isolation and characterization of a hydroxyacid-oxoacid transhydrogenase from rat kidney mitochondria.
abstract: A transhydrogenase that catalyzes the oxoacid-dependent oxidation of specific hydroxyacids has been found in rat kidney, liver, and brain. The hydroxyacids that have been found to be substrates for this enzyme are gamma-hydroxybutyrate, D-alpha-hydroxyglutarate, and L-beta-hydroxybutyrate. The oxoacids that are the best substrates for this enzyme are alpha-ketoglutarate and succinic semialdehyde; alpha-ketoadipate and oxalacetate are also substrates. This enzyme is located in the mitochondrial fraction of the cell and is not dependent on added NAD+ or NADP+.
authors.affiliation: Department of Health and Human Services, National Institute of Mental Health, Bethesda, Maryland 20892.
authors.forename: E E
authors.initials: EE
authors.lastname: Kaufman
authors.forename: T
authors.initials: T
authors.lastname: Nelson
authors.forename: H M
authors.initials: HM
authors.lastname: Fales
authors.forename: D M
authors.initials: DM
authors.lastname: Levin
mesh_terms: D020155:3-Hydroxybutyric Acid; D000818:Animals; D002854:Chromatography, Paper; D008401:Gas Chromatography-Mass Spectrometry; D005977:Glutarates / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D006885:Hydroxybutyrates / Q000378:metabolism; D007668:Kidney / Q000201:enzymology* / Q000648:ultrastructure; D007700:Kinetics; D008928:Mitochondria / Q000201:enzymology; D009243:NAD / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D051381:Rats; D012978:Sodium Oxybate / Q000378:metabolism; D013997:Time Factors; D005680:gamma-Aminobutyric Acid / Q000031:analogs & derivatives / Q000378:metabolism
chemical_list: D005977:Glutarates; D006885:Hydroxybutyrates; D009243:NAD; C019417:alpha-hydroxyglutarate; D009249:NADP; D005680:gamma-Aminobutyric Acid; D012978:Sodium Oxybate; C009338:succinic semialdehyde; D020155:3-Hydroxybutyric Acid