title: Sequencing proteins from acrylamide gels.
abstract: The analytical power of high-resolution two-dimensional gel electrophoresis has been coupled with molecular cloning techniques to allow the sequencing of proteins directly from preparative gels.
authors.affiliation: Howard Hughes Medical Institute, College of Physicians and Surgeons, Columbia University, New York, New York 10032.
authors.forename: T E
authors.initials: TE
authors.lastname: Kennedy
authors.forename: K
authors.initials: K
authors.lastname: Wager-Smith
authors.forename: A
authors.initials: A
authors.lastname: Barzilai
authors.forename: E R
authors.initials: ER
authors.lastname: Kandel
authors.forename: J D
authors.initials: JD
authors.lastname: Sweatt
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001048:Aplysia / Q000032:analysis; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D015342:DNA Probes; D004591:Electrophoresis, Polyacrylamide Gel; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000302:isolation & purification; D011506:Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D012697:Serine Endopeptidases / Q000378:metabolism
chemical_list: D015342:DNA Probes; D010446:Peptide Fragments; D011506:Proteins; D004247:DNA; D012697:Serine Endopeptidases; C028344:glutamyl endopeptidase