The use of subchromosome-length unique band sequences in the analysis of prophase chromosomes.
Using human prophase chromosome ideograms at the 850-band stage, we previously demonstrated that the 24 prophase ideograms can be divided into a set of 94 unique band sequences, each having a recognizable banding pattern distinct from other nonhomologous chromosome portions. Using actual prophase mitotic cells in this study, we analyzed the p arm of chromosome 11 and of chromosomes 16-22 and characterized a similar set of unique band sequences on actual chromosomes. This set of unique band sequences, a statistical comparison scheme, and image-processing techniques outlined in the present report can be used to identify and distinguish banding patterns of these chromosomes and to determine band pattern abnormalities.
Lockwood
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DH
Graduate School of Biomedical Sciences, University of Texas Health Science Center, Houston.
Johnston
D A
DA
Riccardi
V M
VM
Zimmerman
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SO
D002869:Chromosome Aberrations; D002871:Chromosome Banding / Q000379:methods*; D002877:Chromosomes, Human / Q000648:ultrastructure*; D006801:Humans; D007621:Karyotyping; D011418:Prophase