Adenine nucleotide metabolism of blood platelets in human liver cirrhosis.
The adenylate energy charge of human platelets was measured in normal subjects and cirrhotic patients using a method by labelling the adenine nucleotide pool of platelets with radioactive adenine. Using the above method, it was demonstrated that a reliable value for the adenylate energy charge can be obtained from platelet rich plasma containing more than 10(4) platelets per microliter after 30 minutes incubation. A significant difference was found in the distribution of adenine nucleotide metabolites between normal subjects and cirrhotic patients. However, no significant difference was found in the adenylate energy charge between two groups. These results indicate that energy metabolism of platelets is impaired to some extent in liver cirrhosis.
Zaima
M
M
Second Department of Surgery, Kyoto University Medical School, Japan.
Noguchi
M
M
Wada
Y
Y
Mori
K
K
Ozawa
K
K
D000227:Adenine Nucleotides / Q000097:blood / Q000378:metabolism*; D000368:Aged; D001792:Blood Platelets / Q000378:metabolism*; D004734:Energy Metabolism; D005260:Female; D006801:Humans; D008103:Liver Cirrhosis / Q000097:blood* / Q000378:metabolism; D008297:Male; D008875:Middle Aged
D000227:Adenine Nucleotides