title: Isolation and characterization of valine dehydrogenase from Streptomyces aureofaciens.
abstract: Valine dehydrogenase was purified to homogeneity from the crude extracts of Streptomyces aureofaciens. The molecular weight of the native enzyme was 116,000 by equilibrium ultracentrifugation and 118,000 by size exclusion high-performance liquid chromatography. The enzyme was composed of four subunits with molecular weights of 29,000. The isoelectric point was 5.1. The enzyme required NAD+ as a cofactor, which could not be replaced by NADP+. Sulfhydryl reagents inhibited the enzyme activity. The pH optimum was 10.7 for oxidative deamination of L-valine and 9.0 for reductive amination of alpha-ketoisovalerate. The Michaelis constants were 2.5 mM for L-valine and 0.10 mM for NAD+. For reductive amination the Km values were 1.25 mM for alpha-ketoisovalerate, 0.023 mM for NADH, and 18.2 mM for NH4Cl.
authors.affiliation: Institute of Microbiology, Czechoslovak Academy of Sciences, Prague.
authors.forename: I
authors.initials: I
authors.lastname: Vancurová
authors.forename: A
authors.initials: A
authors.lastname: Vancura
authors.forename: J
authors.initials: J
authors.lastname: Volc
authors.forename: J
authors.initials: J
authors.lastname: Neuzil
authors.forename: M
authors.initials: M
authors.lastname: Flieger
authors.forename: G
authors.initials: G
authors.lastname: Basarová
authors.forename: V
authors.initials: V
authors.lastname: Bĕhal
mesh_terms: D000594:Amino Acid Oxidoreductases / Q000302:isolation & purification* / Q000378:metabolism; D004795:Enzyme Stability; D007700:Kinetics; D046911:Macromolecular Substances; D008970:Molecular Weight; D013302:Streptomyces / Q000201:enzymology* / Q000254:growth & development; D013379:Substrate Specificity
chemical_list: D046911:Macromolecular Substances; D000594:Amino Acid Oxidoreductases; C057545:valine dehydrogenase (NAD+)