title: Seronegative virus carriers in the infection of rabbits with human T lymphotropic virus type I.
abstract: Six HTLV-I-transformed T cell lines were prepared from PBL of three rabbits each of B/J and Chbb:HM strains, and were inoculated into newborn rabbits of these two strains, and of their F1 hybrid. None of three B/J cell lines induced anti-HTLV-I antibody response in newborn B/J rabbits, whereas all three Chbb:HM cell lines did induce a response in newborn Chbb:HM rabbits. These B/J cell lines however could induce antibody response in adult B/J as well as newborn Chbb:HM rabbits, and a Chbb:HM cell line could induce a response in a newborn B/J rabbit. Similar unresponsiveness was observed in (B/J x Chbb:HM)F1 hybrids neonatally inoculated with B/J cells. Unresponsiveness was abrogated by reinoculation of some but not other cell lines. Viral antigen-positive cell lines harboring HTLV-I provirus genomes were established from such seronegative B/J and F1 rabbits. Simultaneous inoculation of HTLV-I-transformed cells and SPV resulted in the induction of papilloma and antibody against SPV, but not antibody against HTLV-I. The present findings thus reveal that neonatal infection of HTLV-I could result in immunological tolerance to the virus antigens, thereby leading to a persistent infection without antibody induction.
authors.affiliation: Department of Microbiology, Shiga University of Medical Science, Otsu, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Seto
authors.forename: M
authors.initials: M
authors.lastname: Kawanishi
authors.forename: S
authors.initials: S
authors.lastname: Matsuda
authors.forename: K
authors.initials: K
authors.lastname: Ogawa
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D000914:Antibodies, Viral / Q000032:analysis*; D002353:Carrier State; D004195:Disease Models, Animal; D015490:HTLV-I Infections / Q000276:immunology*; D011817:Rabbits
chemical_list: D000914:Antibodies, Viral