Ribosome-inactivating proteins from plants inhibit ribosome activity of Trypanosoma and Leishmania.
Ribosomes from Trypanosoma brucei rhodesiense and from Leishmania infantum were isolated and optimal conditions for in vitro translation were established. The effect of ribosome-inactivating proteins extracted from several plants was then assessed in order to identify those suitable for the preparation of immunotoxins against these organisms. Ribosomes from both species were inactivated by some ribosome-inactivating proteins (dianthins, saporins, pokeweed antiviral proteins, and the ribosome-inactivating chain of abrin). The similarity of the effects on the ribosomes from the two species examined indicates that ribosome-inactivating proteins should also be effective in a similar way on ribosomes from other species of Trypanosoma and Leishmania.
Cenini
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Dipartimento di Patologia sperimentale, Università di Bologna, Italy.
Bolognesi
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A
Stirpe
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D000036:Abrin / Q000494:pharmacology; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D000998:Antiviral Agents / Q000494:pharmacology; D000922:Immunotoxins; D007893:Leishmania donovani / Q000187:drug effects* / Q000235:genetics; D009699:N-Glycosyl Hydrolases; D010940:Plant Proteins / Q000494:pharmacology*; D014176:Protein Biosynthesis / Q000187:drug effects; D054789:Ribosome Inactivating Proteins, Type 1; D012270:Ribosomes / Q000187:drug effects* / Q000502:physiology; D000076985:Saporins; D014346:Trypanosoma brucei brucei / Q000187:drug effects* / Q000235:genetics
D000972:Antineoplastic Agents, Phytogenic; D000998:Antiviral Agents; D000922:Immunotoxins; D010940:Plant Proteins; D054789:Ribosome Inactivating Proteins, Type 1; D000036:Abrin; D009699:N-Glycosyl Hydrolases; D000076985:Saporins; C024275:pokeweed antiviral protein