Protection against complement-mediated cell damage by Ca2+ and Zn2+.
Ca2+ and Zn2+ prevent antibody-dependent complement-induced permeability changes in tonsil lymphocytes and Lettre cells. Lactate dehydrogenase leaks out from Lettre cells at high complement:cell ratios, under which conditions higher concentrations of Ca2+ and Zn2+ are required for protection. Ca2+ and Zn2+ do not inhibit complement activation or C9 binding to Lettre cells, and prevent leakage through preformed lesions. It is concluded that the extent of complement-induced membrane damage depends on the concentration of extracellular Ca2+, and may be modulated by changes in extracellular Ca2+ or Zn2+.
Micklem
K J
KJ
Nuffield Department of Pathology, John Radcliffe Hospital, Oxford, UK.
Alder
G M
GM
Buckley
C D
CD
Murphy
J
J
Pasternak
C A
CA
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002463:Cell Membrane Permeability / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003176:Complement C3 / Q000032:analysis; D003186:Complement C9 / Q000378:metabolism; D003169:Complement Inactivator Proteins / Q000494:pharmacology*; D003165:Complement System Proteins / Q000502:physiology*; D006461:Hemolysis; D008214:Lymphocytes / Q000187:drug effects; D051379:Mice; D015032:Zinc / Q000494:pharmacology
D003176:Complement C3; D003186:Complement C9; D003169:Complement Inactivator Proteins; D003165:Complement System Proteins; D015032:Zinc; D002118:Calcium