title: Importance of anion in hypotonic volume regulation of rabbit proximal straight tubule.
abstract: Rabbit proximal straight tubules rapidly swell to a maximum volume when abruptly immersed into hypotonic medium. However, in a second, slower phase, termed volume regulatory decrease (VRD), tubules shrink toward their basal volume due to the efflux of K, accompanying anion and water. In the present study, we investigated the nature of the anion during hypotonic volume regulation. We removed Cl and/or the HCO3 buffer to assess their relative importance in VRD, and we used furosemide, 4-acetamido-4'-isothiocyanostilbene-2,2'-disulfonic acid (SITS), and barium to investigate the nature of the transmembrane anion pathway in VRD. Isosmotic replacement of peritubular Cl with gluconate had no effect on either tubule volume in isotonic medium or the initial osmometric swelling response in hypotonic medium. However, the VRD in such Cl-depleted tubules was significantly inhibited. Control tubules regulated 81% below their maximal volume in dilute medium. By contrast, Cl-depleted tubules regulated only 39%. This inhibitory effect could not be attributed to the absence of peritubular Cl or to the presence of gluconate. The absence of the HCO3 buffer or the presence of SITS (0.5 mM) had no inhibitory effect on the rate or extent of VRD. Furosemide alone (1 mM) also had no inhibitory effect on VRD. However, whereas barium alone delays VRD, addition of furosemide to barium-treated tubules further slowed their maximal rate of fluid efflux and delayed VRD even more.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Pediatrics, University of Connecticut Health Center, Farmington 06032.
authors.forename: P A
authors.initials: PA
authors.lastname: Welling
authors.forename: M A
authors.initials: MA
authors.lastname: Linshaw
mesh_terms: D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid; D000818:Animals; D000838:Anions; D001464:Barium / Q000494:pharmacology; D001639:Bicarbonates / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D002712:Chlorides / Q000378:metabolism*; D005260:Female; D005665:Furosemide / Q000494:pharmacology; D007038:Hypotonic Solutions; D007687:Kidney Tubules, Proximal / Q000033:anatomy & histology / Q000187:drug effects / Q000502:physiology*; D007700:Kinetics; D011188:Potassium / Q000378:metabolism; D011817:Rabbits; D014882:Water-Electrolyte Balance / Q000187:drug effects
chemical_list: D000838:Anions; D001639:Bicarbonates; D002712:Chlorides; D007038:Hypotonic Solutions; D001464:Barium; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid; D005665:Furosemide; D011188:Potassium