Stimulation of neurite outgrowth in vitro by a glycero-ganglioside.
A glycerol-containing analog of ganglioside, with sialic acid attached to a diglyceride-like structure possessing two ether-linked alkyl chains, was prepared synthetically and applied exogenously to three culture systems; neuro-2A neuroblastoma cells, PC12 cells and dorsal root ganglia. This resulted in pronounced stimulation of neurite outgrowth in all three, demonstrating that sialo-lipids(s) lacking ceramide and possessing sialic acid as the sole carbohydrate are able to promote neuritogenesis in approximately the same manner as naturally occurring gangliosides.
Cannella
M S
MS
Department of Neurology, Albert Einstein College of Medicine, Bronx, NY 10461.
Acher
A J
AJ
Ledeen
R W
RW
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D002642:Chick Embryo; D003712:Dendrites / Q000187:drug effects; D004075:Diglycerides / Q000494:pharmacology*; D005727:Ganglia, Spinal / Q000166:cytology* / Q000187:drug effects; D005732:Gangliosides / Q000494:pharmacology*; D005989:Glycerides / Q000494:pharmacology*; D009414:Nerve Growth Factors / Q000494:pharmacology*; D009447:Neuroblastoma; D010455:Peptides / Q000494:pharmacology; D010673:Pheochromocytoma; D014407:Tumor Cells, Cultured
D004075:Diglycerides; D005732:Gangliosides; D005989:Glycerides; D009414:Nerve Growth Factors; D010455:Peptides; C047805:neurite-inducing factor; C057475:1,2-ditetradecylglycerol