title: Cytotoxic effects of müllerian inhibiting substance (MIS)?
abstract: To determine whether Müllerian Inhibiting Substance (MIS) is the factor responsible for the cytotoxic effects of testicular preparations on gynecological cancers, homogenates of bovine fetal testes were fractionated by Sephadex G 200 chromatography and their cytotoxic effects tested on a Müllerian-derived endometrial cancer cell line (AN3-CA), an ovarian carcinoma cell line (CAOV 3) and on a breast cancer cell line (MCF 7). No difference in cytotoxicity was found between Müllerian and non-Müllerian derived human cancer cells. Cytotoxicity and MIS activity were found in different fractions on Sephadex G 200 gel chromatography.
authors.affiliation: Department of Gynecological Oncology, University of Leuven, Belgium.
authors.forename: J P
authors.initials: JP
authors.lastname: Janssens
authors.forename: V
authors.initials: V
authors.lastname: Noto
authors.forename: G
authors.initials: G
authors.lastname: van Tendeloo
authors.forename: J
authors.initials: J
authors.lastname: Bonte
authors.forename: W
authors.initials: W
authors.lastname: de Loecker
mesh_terms: D054304:Anti-Mullerian Hormone; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D005260:Female; D005833:Genital Neoplasms, Female; D006023:Glycoproteins; D006131:Growth Inhibitors; D006801:Humans; D009095:Mullerian Ducts; D013735:Testicular Hormones / Q000494:pharmacology*
chemical_list: D006023:Glycoproteins; D006131:Growth Inhibitors; D013735:Testicular Hormones; D054304:Anti-Mullerian Hormone