Polarity of neutral glycolipids, gangliosides, and sulfated lipids in MDCK epithelial cells.
Confluent monolayers of MDCK (Madin-Darby canine kidney) cells provide a widely used model system for studying epithelial cell polarity. We determined the polarity of epithelial cell plasma membrane glycolipids and sulfated lipids by analyzing the lipids released from both sides of monolayers of metabolically labeled MDCK cells. These lipids were released either as endogenously shed material or in budding viruses. All of the glycolipids were detected in both the apical and basolateral domains of the plasma membrane. However, galactosylceramide was more basally oriented than any of the other glycolipids; thus, the ratio of glucosylceramide to galactosylceramide was more than twice as great in the apical domain as in the basolateral domain. A sulfated sterol, which comigrated with cholesterol sulfate, was released in a more basally polarized manner than any of the glycolipids. These results indicate the presence of mechanisms which can produce different degrees of polarity for specific lipids in polarized epithelial cells.
Nichols
G E
GE
Department of Biochemistry, University of Virginia Medical Center, Charlottesville 22908.
Shiraishi
T
T
Young
W W
WW
D000818:Animals; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D002999:Clone Cells; D004285:Dogs; D004847:Epithelial Cells; D004848:Epithelium / Q000032:analysis; D005732:Gangliosides / Q000032:analysis*; D006017:Glycolipids / Q000032:analysis*; D008055:Lipids / Q000032:analysis*; D013431:Sulfates / Q000032:analysis
D005732:Gangliosides; D006017:Glycolipids; D008055:Lipids; D013431:Sulfates