Induction of single-strand breaks in liver DNA of mice after inhalation of vinyl chloride.
NMRI female mice were exposed to 100, 250 and 500 ppm vinyl chloride (VC). Cell nuclei were prepared from the liver, and single-strand breaks (SSB) were determined by the DNA unwinding technique. Haemoglobin (Hb) was isolated from the blood, and the degree of alkylation was determined as a measure of in-vivo dose by means of a gas chromatography-mass spectrometry (GC-MS) technique. A maximum level of SSB in liver DNA and of adduct levels of Hb was reached at 500 ppm, indicating that saturation of metabolic activation of VC had been achieved. The results demonstrate that VC induces SSB in liver DNA of mice in a dose-dependent manner and that about 80% of the damage is repaired within 20 h.
Walles
S A
SA
National Institute of Occupational Health, Solna, Sweden.
Holmberg
B
B
Svensson
K
K
Osterman-Golkar
S
S
Sigvardsson
K
K
Lindblom
K
K
D000280:Administration, Inhalation; D000818:Animals; D004249:DNA Damage; D004277:DNA, Single-Stranded / Q000187:drug effects*; D005260:Female; D008099:Liver / Q000187:drug effects*; D051379:Mice; D014752:Vinyl Chloride / Q000633:toxicity*; D014753:Vinyl Compounds / Q000633:toxicity*
D004277:DNA, Single-Stranded; D014753:Vinyl Compounds; D014752:Vinyl Chloride