title: Phosphatidylcholine synthesis in platelets is stimulated by diacylglycerol but not by phorbol ester.
abstract: The biosynthesis of phosphatidylcholine (PC) in platelets was followed by measuring the incorporation of 32Pi. Incorporation into PC was stimulated by treatment with Clostridium perfringens phospholipase C or with the synthetic diacylglycerol sn-1,2-dioctanoylglycerol. However, neither the phorbol ester tumour promoter 12-O-tetradecanoylphorbol-13-acetate or thrombin stimulated 32Pi incorporation into PC. We conclude that phorbol ester does not stimulate the hydrolysis of PC to diacylglycerol in platelets.
authors.affiliation: School of Biological Sciences, Flinders University, Bedford Park, South Australia.
authors.forename: J G
authors.initials: JG
authors.lastname: Muir
authors.forename: A W
authors.initials: AW
authors.lastname: Murray
mesh_terms: D001792:Blood Platelets / Q000378:metabolism*; D004075:Diglycerides / Q000494:pharmacology*; D005989:Glycerides / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D010703:Phorbol Esters / Q000494:pharmacology*; D010713:Phosphatidylcholines / Q000096:biosynthesis*; D010743:Phospholipids / Q000096:biosynthesis; D013268:Stimulation, Chemical; D013917:Thrombin / Q000494:pharmacology
chemical_list: C011439:1,2-diacylglycerol; D004075:Diglycerides; D005989:Glycerides; D010703:Phorbol Esters; D010713:Phosphatidylcholines; D010743:Phospholipids; D013917:Thrombin