Zinc-binding proteins detected by protein blotting.
The Western blotting technique was used for the detection of zinc-binding proteins. Proteins were separated electrophoretically on 15% polyacrylamide-sodium dodecyl sulfate minigels, the gels were soaked in a reduction buffer, and the proteins were transferred to nitrocellulose filters. Zinc-binding proteins were probed with radioactive zinc (65Zn) and were detected by autoradiography. This technique allows the detection of as little as 20 to 100 pmol of zinc metalloproteins.
Mazen
A
A
Institut de Biologie Moléculaire et Cellulaire du CNRS, Strasbourg, France.
Gradwohl
G
G
de Murcia
G
G
D001345:Autoradiography; D015153:Blotting, Western; D002352:Carrier Proteins / Q000032:analysis*; D004268:DNA-Binding Proteins / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D008667:Metalloproteins / Q000032:analysis; D010446:Peptide Fragments / Q000032:analysis; D015037:Zinc Radioisotopes
D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D008667:Metalloproteins; D010446:Peptide Fragments; D015037:Zinc Radioisotopes; C017800:zinc-binding protein