Molecular cloning of two types of GAP complementary DNA from human placenta.
The ras p21 GTPase-activating protein (GAP) was purified from human placental tissue. Internal amino acid sequence was obtained from this 120,000-dalton protein and, by means of this sequence, two types of complementary DNA clones were isolated and characterized. One type encoded GAP with a predicted molecular mass of 116,000 daltons and 96% identity with bovine GAP. The messenger RNA of this GAP was detected in human lung, brain, liver, leukocytes, and placenta. The second type appeared to be generated by a differential splicing mechanism and encoded a novel form of GAP with a predicted molecular mass of 100,400 daltons. This protein lacks the hydrophobic amino terminus characteristic of the larger species, but retains GAP activity. The messenger RNA of this type was abundantly expressed in placenta and in several human cell lines, but not in adult tissues.
Trahey
M
M
Department of Molecular Biology, Cetus Corp., Emeryville, CA 94608.
Wong
G
G
Halenbeck
R
R
Rubinfeld
B
B
Martin
G A
GA
Ladner
M
M
Long
C M
CM
Crosier
W J
WJ
Watt
K
K
Koths
K
K
D000595:Amino Acid Sequence; D001483:Base Sequence; D001923:Brain Chemistry; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D005260:Female; D020690:GTPase-Activating Proteins; D005786:Gene Expression Regulation; D006801:Humans; D007962:Leukocytes / Q000032:analysis; D008099:Liver / Q000032:analysis; D008168:Lung / Q000032:analysis; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D010920:Placenta / Q000032:analysis*; D011247:Pregnancy; D011506:Proteins / Q000235:genetics* / Q000302:isolation & purification; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D020703:ras GTPase-Activating Proteins
D020690:GTPase-Activating Proteins; D015345:Oligonucleotide Probes; D011506:Proteins; D012333:RNA, Messenger; D020703:ras GTPase-Activating Proteins; D004247:DNA