Clonogenic growth of acute non-lymphocytic leukemia cells in serum-free medium.
We devised a serum-free medium for growth of leukemic colony-forming units (CFU-L), enriched with albumin, transferrin, lipids, insulin, hydrocortisone and oligoelements. Blast cells from 15 patients affected by acute non-lymphocytic leukemia were grown in this medium in the presence of human placental conditioned medium obtained under serum-free conditions (sfHPCM). Their clonogenic growth was comparable with that obtained in a serum-containing system. Furthermore, when serum-free cultures were carried out in absence of sfHPCM, either CFU-L growth was prevented or, if clones were obtained, the cultures showed a marked decrease in clonogenicity, indicating their strict dependence on growth factors.
Bernabei
P A
PA
Università degli Studi di Firenze, Policlinico di Careggi, Italy.
Santini
V
V
Dal Pozzo
O
O
Bezzini
R
R
Gattei
V
V
Saccardi
R
R
Rombolà
G
G
Ferrini
P R
PR
D000328:Adult; D001769:Blood; D002455:Cell Division; D002999:Clone Cells; D003470:Culture Media; D006133:Growth Substances; D006412:Hematopoietic Stem Cells / Q000473:pathology; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000473:pathology*; D008055:Lipids; D010920:Placenta; D014407:Tumor Cells, Cultured
D003470:Culture Media; D006133:Growth Substances; D008055:Lipids