title: Messenger RNA expressed in mouse teratocarcinoma stem cells and down-regulated by a tumor-promoting phorbol ester codes for a novel transmembrane protein.
abstract: Cloning and sequence analysis of a DNA complementary to the mRNA expressed in undifferentiated mouse F9 teratocarcinoma stem cells but disappearing rapidly after treatment with a tumor-promoting phorbol ester revealed it to be a 1.9 kilobase pairs-long cDNA encoding a protein of 323 amino acid residues. Computer-assisted analyses of the deduced amino acid sequence indicated that this protein contains a typical hydrophobic signal peptide consisting of 33 amino acid residues and six putative membrane-spanning segments. The deduced amino acid sequence, as a whole, bears no significant sequence homology to any previously described protein.
authors.affiliation: Department of Biochemistry, Kumamoto University Medical School, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Akagi
authors.forename: H
authors.initials: H
authors.lastname: Nomiyama
authors.forename: C
authors.initials: C
authors.lastname: Setoyama
authors.forename: K
authors.initials: K
authors.lastname: Shimada
authors.forename: M
authors.initials: M
authors.lastname: Akagi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects; D008565:Membrane Proteins / Q000235:genetics* / Q000648:ultrastructure; D051379:Mice; D008969:Molecular Sequence Data; D013724:Teratoma / Q000235:genetics; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D008565:Membrane Proteins; D004247:DNA; D013755:Tetradecanoylphorbol Acetate