title: [Gamma-cystathionase: the non-ideal gel filtration high performance liquid chromatography. Physico-chemical and immunochemical characteristics of the enzyme].
abstract: The rapid method for gamma-cystathionase purification was developed. It is based on the non-ideal gel filtration HPLC. The isolated homogeneous enzyme was used for immunization and immunosorbent preparation. A monospecific polyclonal antibody was prepared. The substrate specificity of the isolated enzyme was studied.
authors.forename: A G
authors.initials: AG
authors.lastname: Gabikov
authors.forename: A M
authors.initials: AM
authors.lastname: Shuster
authors.forename: V K
authors.initials: VK
authors.lastname: Kazakov
authors.forename: N N
authors.initials: NN
authors.lastname: Kondrashina
authors.forename: E V
authors.initials: EV
authors.lastname: Goriachenkova
mesh_terms: D000911:Antibodies, Monoclonal; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003542:Cystathionine gamma-Lyase / Q000276:immunology / Q000302:isolation & purification*; D004247:DNA / Q000235:genetics; D015151:Immunoblotting; D008190:Lyases / Q000302:isolation & purification*; D013379:Substrate Specificity
chemical_list: D000911:Antibodies, Monoclonal; D004247:DNA; D008190:Lyases; D003542:Cystathionine gamma-Lyase
vernacular_title: Gamma-tsistationaza: vysokoéffektivnaia zhidkostnaia khromatografiia v rezhime neideal'noĭ gel'-fil'tratsii. Fiziko-khimicheskaia i immunokhimicheskaia kharakteristika fermenta.