title: Characterization, localization, and biosynthesis of acetylcholinesterase in K 562 cells.
abstract: K 562 cell acetylcholinesterase (AChE), identifiable by active site labeling with radioactive diisopropylfluorophosphate (DFP), showed a Mr around 55,000 in both a crude lysate and a purified sample. The K 562 AChE was reactive with one polyclonal and two monoclonal antibodies produced against human erythrocyte AChE. Subcellular localization, investigated by assay on cell fractions, showed that AChE is membrane bound and that it is located on the cell surface as well as on microsomal and Golgi membranes. Biosynthesis of new enzyme molecules, after inactivation of the constitutive AChE with the irreversible inhibitor DFP, allowed us to follow the kinetics of reappearance in the intracellular compartment and at the cell surface (4 and 8 h, respectively).
authors.affiliation: Department of Biology, University of Genova, I.S.M.I., Italy.
authors.forename: R
authors.initials: R
authors.lastname: Ravazzolo
authors.forename: C
authors.initials: C
authors.lastname: Garré
authors.forename: G
authors.initials: G
authors.lastname: Bianchi-Scarrá
authors.forename: R
authors.initials: R
authors.lastname: Barresi
authors.forename: G
authors.initials: G
authors.lastname: Damiani
authors.forename: V
authors.initials: V
authors.lastname: Capra
authors.forename: F
authors.initials: F
authors.lastname: Ajmar
mesh_terms: D000110:Acetylcholinesterase / Q000096:biosynthesis*; D000345:Affinity Labels; D001665:Binding Sites; D002462:Cell Membrane / Q000201:enzymology; D007700:Kinetics; D007942:Leukemia, Experimental / Q000201:enzymology*; D013050:Spectrometry, Fluorescence; D013347:Subcellular Fractions / Q000201:enzymology; D014407:Tumor Cells, Cultured
chemical_list: D000345:Affinity Labels; D000110:Acetylcholinesterase