Detection of DNA adducts by postlabelling with 3H-acetic anhydride.
A method has been developed for detecting 7-alkylguanines which is based on derivatization of hydroxyl and primary amino groups with 3H-acetic anhydride. The derivatization procedure is devised for 7-methylguanine, with examination of reaction kinetics. The method is applied to the detection of the isomeric styrene oxide-guanine adducts; styrene-7,8-oxide is the active metabolite of styrene. The procedure can be used as a postlabelling method for the simultaneous detection of several adducts after selective depurination of 7-alkylguanines from DNA.
Hemminki
K
K
Institute of Occupational Health, Helsinki, Finland.
Savela
K
K
Linkola
E
E
Hesso
A
A
D000089:Acetic Anhydrides; D000107:Acetylation; D004852:Epoxy Compounds / Q000378:metabolism*; D004988:Ethers, Cyclic / Q000378:metabolism*; D006147:Guanine / Q000031:analogs & derivatives* / Q000032:analysis; D006151:Guanosine / Q000378:metabolism*; D014316:Tritium
D000089:Acetic Anhydrides; D004852:Epoxy Compounds; D004988:Ethers, Cyclic; D014316:Tritium; D006151:Guanosine; C031800:acetic anhydride; D006147:Guanine; C008450:7-methylguanine; C013690:styrene oxide