Hydrolysis of platelet activating factor and its methylated analogs by acetylhydrolases.
We examined the substrate specificity of PAF-degrading enzymes from various sources using platelet activating factor (PAF) and its synthetic analogs. The results were as follows: 1) Tissue-originated acetylhydrolases, such as rat kidney soluble enzyme, deacetylated 1S-methyl-1-O-hexadecyl-2-acetyl-sn-glycero-3-phosphocholine (1S-Me-PAF) slightly more rapidly than PAF, whereas plasma acetylhydrolase hydrolyzed PAF more effectively than 1S-Me-PAF. 2) Rat polymorphonuclear leukocytes, monocytes, and lymphocytes homogenates showed an appreciable acetylhydrolase activity, the substrate specificity of which resembled that of the plasma enzyme. 3) Pleural exudates in an experimental pleurisy induced in rats by carrageenan contained an acetylhydrolase activity, the properties of which were similar to those of the plasma enzyme. 4) An extracellular phospholipase A2 activity, which was also observed in the pleural exudate and required Ca2+ ion for maximum activity, seemed not to participate in the deacetylation of PAF, since addition of EDTA did not affect the PAF deacetylation catalyzed by the pleural exudate. These findings indicate that the inactivation reaction of PAF present in the extracellular space is mainly catalyzed by plasma acetylhydrolase, which yields lysoPAF.
Yanoshita
R
R
Faculty of Pharmaceutical Sciences, University of Tokyo.
Kudo
I
I
Ikizawa
K
K
Chang
H W
HW
Kobayashi
S
S
Ohno
M
M
Nojima
S
S
Inoue
K
K
D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase; D000818:Animals; D001773:Blood Cells / Q000201:enzymology; D006168:Guinea Pigs; D006801:Humans; D006868:Hydrolysis; D007668:Kidney / Q000201:enzymology; D008297:Male; D010740:Phospholipases / Q000378:metabolism*; D010741:Phospholipases A / Q000097:blood / Q000378:metabolism*; D054467:Phospholipases A2; D010972:Platelet Activating Factor / Q000031:analogs & derivatives* / Q000378:metabolism*; D010998:Pleurisy / Q000139:chemically induced / Q000201:enzymology; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains; D013379:Substrate Specificity
D010972:Platelet Activating Factor; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2; D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase