Selective removal of low-density lipoproteins from plasma by polyacrylate-coated Fractogel in vitro and in experimental extracorporeal perfusion.
Polyacrylate, covalently bound to Fractogel, was tested as a sorbent for lipoproteins from human plasma in a perfusion column. Experiments in vitro indicated complete elimination of low-density lipoproteins from citrated plasma. No binding of high-density lipoproteins, fibrinogen, or the complement factors C3 and C4 was observed. Biocompatibility was demonstrated by extracorporeal plasma perfusion in sheep. The results indicate the potential use of polyacrylate-coated Fractogel for the removal of low-density lipoproteins from plasma ex vivo.
Thies
K
K
Institut für Immunologie und Serologie, Universität Heidelberg, F.R.G.
Prigent
S A
SA
Heuck
C C
CC
D000180:Acrylic Resins; D000818:Animals; D000990:Antithrombin III / Q000378:metabolism; D001780:Blood Coagulation Tests; D002118:Calcium / Q000097:blood; D002784:Cholesterol / Q000097:blood; D005340:Fibrinogen / Q000378:metabolism; D006464:Hemoperfusion / Q000295:instrumentation*; D008075:Lipoproteins, HDL / Q000097:blood; D008077:Lipoproteins, LDL / Q000097:blood*; D008079:Lipoproteins, VLDL / Q000097:blood; D011108:Polymers; D012756:Sheep; D014280:Triglycerides / Q000097:blood
D000180:Acrylic Resins; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D008079:Lipoproteins, VLDL; D011108:Polymers; D014280:Triglycerides; C006903:carbopol 940; C049226:fractogel TSK HW 40S; D000990:Antithrombin III; D005340:Fibrinogen; D002784:Cholesterol; D002118:Calcium