The OVEIA assay, a simultaneous measurement of oestrone and pregnanediol glucuronides, for studying the treatment of infertility patients.
A new simple assay developed for establishing the limits of the fertile period was evaluated in infertility patients. This assay is a colorimetric method for measuring the oestrone glucuronide/pregnanediol glucuronide ratio. Steroid levels were assayed simultaneously and an index of their ratio was obtained in this dual analyte assay (DAA). Twenty subfertile women, receiving clomiphene citrate for ovulation induction, were monitored for the DAA index using early morning urine samples. Ten of these women were also monitored in a pretreatment cycle. A further 10 patients on an in-vitro fertilization (IVF) programme were assessed with the same assay. DAA ratios were found to correlated with follicular phase serum oestradiol levels. Peak DAA ratios dropped dramatically when significant rises in urinary luteinizing hormone concentrations occurred prior to ovulation. An association between high DAA ratios and adequate luteal phase activity was also established. In addition, the total volume of the three largest ovarian follicles, as measured by ultrasound, in IVF patients was found to correlate with DAA measurements.
Anthony
F
F
Department of Human Reproduction and Obstetrics, University of Southampton, Princess Anne Hospital, UK.
Aldrich
C
C
Sharp
N C
NC
Smith
E M
EM
Masson
G M
GM
Dennis
K J
KJ
D000328:Adult; D002996:Clomiphene / Q000627:therapeutic use*; D004797:Enzyme-Linked Immunosorbent Assay; D004970:Estrone / Q000031:analogs & derivatives* / Q000652:urine; D005260:Female; D006801:Humans; D007247:Infertility, Female / Q000188:drug therapy* / Q000652:urine; D011276:Pregnanediol / Q000031:analogs & derivatives* / Q000652:urine
C022270:pregnanediol-3 alpha-glucuronide; D002996:Clomiphene; C015856:estrone-3-glucuronide; D004970:Estrone; D011276:Pregnanediol