Methods for the detection of Jk heterozygotes: interpretations and applications.
The lytic properties of red cells from Jk(a+,b+), Jk(a-, b+) and Jk(a+b-) (normal), Jk(a-b-), and obligate Jk heterozygotes were studied. The Jk(a-b-) cells did not hemolyze for at least 15 minutes in either 2 M urea or methylurea, whereas normal cells were completely hemolyzed in 2 minutes. Red cells from Jk heterozygotes demonstrated intermediate levels of hemolysis when compared to normal and Jk(a-b-) red cells. In addition, these cells had less than 10 percent hemolysis when suspended in 2 M methylurea prepared in 0.4 percent phosphate-buffered saline (pH 7.2). This method may be an alternative to hemagglutination titration scoring for the detection of Jk, especially in paternity cases.
Edwards-Moulds
J
J
Division of Biological and Allied Health Sciences, University of Houston-Clear Lake, Texas.
Kasschau
M R
MR
D000438:Alcohols; D001789:Blood Group Antigens / Q000235:genetics*; D002120:Calcium Channel Agonists; D003902:Detergents; D006580:Genetic Carrier Screening / Q000379:methods; D006386:Hemagglutination Tests; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D007667:Kidd Blood-Group System / Q000235:genetics* / Q000276:immunology; D010334:Paternity; D010740:Phospholipases; D014508:Urea
D000438:Alcohols; D001789:Blood Group Antigens; D002120:Calcium Channel Agonists; D003902:Detergents; D007667:Kidd Blood-Group System; D014508:Urea; D010740:Phospholipases