[Mechanism of electron transport in the cholesterol-hydroxylating system of adrenal cortex mitochondria: a triple complex of adrenodoxin reductase, adrenodoxin and cytochrome P-450].
A cross-linked ternary adrenodoxin reductase-adrenodoxin-cytochrome P-450scc complex with an apparent molecular mass of 114 kD was obtained, using N-succinimidyl-6-(4'-azido-2'-nitrophenylamino)-hexanoate. The composition of the cross-linked complex was determined by immunoblotting and radioactivity measurements, using N-ethyl [2.3-14C]maleimide-premodified adrenodoxin. The data obtained suggest that the ternary complex may exist in solution.
Turko
I V
IV
Usanov
S A
SA
Akhrem
A A
AA
Chashchin
V L
VL
D000302:Adrenal Cortex / Q000201:enzymology / Q000378:metabolism*; D000325:Adrenodoxin / Q000378:metabolism*; D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D004579:Electron Transport; D004591:Electrophoresis, Polyacrylamide Gel; D005287:Ferredoxin-NADP Reductase / Q000378:metabolism*; D046911:Macromolecular Substances; D008928:Mitochondria / Q000201:enzymology / Q000378:metabolism*; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism*
D046911:Macromolecular Substances; D000325:Adrenodoxin; D003577:Cytochrome P-450 Enzyme System; D005287:Ferredoxin-NADP Reductase; D009247:NADH, NADPH Oxidoreductases
Mekhanizm élektronnogo transporta v kholesteringidroksiliruiushcheĭ sisteme mitokhondriĭ kory nadpochechnikov: troĭnoĭ kompleks adrenodoksinreduktazy, adrenodoksina i tsitokhroma P-450.