Measurement of specific radioactivity by high-performance liquid chromatograph with a synchronized accumulating radioisotope detector: determination of turnover rate and pool size of tryptophan in rat.
A high-performance liquid chromatograph with a synchronized accumulating radioisotope detector was used to determine the turnover rate and pool size of tryptophan in rat. The specific radioactivity could be followed for three half-lives on the final slope of the specific radioactivity curve following intravenous administration of 15 muCi of carrier free tryptophan labeled with carbon-14. Remarkable individual differences were noted in turnover rate and in pool size among rats.
Baba
S
S
Laboratory of Physical and Pharmaceutical Chemistry, Tokyo College of Pharmacy, Japan.
Yamaguchi
J
J
Horie
M
M
D000818:Animals; D002250:Carbon Radioisotopes; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006207:Half-Life; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D014364:Tryptophan / Q000032:analysis* / Q000378:metabolism
D002250:Carbon Radioisotopes; D014364:Tryptophan