title: Reduction of the number of mice used for potency testing of human and animal rabies vaccines.
abstract: Eight different rabies vaccines were tested for their potency in the standard mouse potency test using 3-, 5- and 7-week-old mice. 5-week-old mice seem to be best suited for this purpose, variability from test to test could be reduced considerably. An ELISA was used in parallel for the evaluation of the rabies glycoprotein content of rabies vaccines. Results of the mouse potency test correlated well with those of the ELISA if highly purified human vaccines were tested. Unspecific reactions in the ELISA caused by adjuvanted veterinary vaccines could not be blocked. Further experiments will be needed in order to evaluate the potency of inactivated veterinary rabies vaccines by a in vitro test.
authors.affiliation: Federal Vaccine Institute, Basel, Switzerland.
authors.forename: L
authors.initials: L
authors.lastname: Bruckner
authors.forename: M
authors.initials: M
authors.lastname: Palatini
authors.forename: M
authors.initials: M
authors.lastname: Ackermann
authors.forename: H K
authors.initials: HK
authors.lastname: Müller
authors.forename: U
authors.initials: U
authors.lastname: Kihm
mesh_terms: D000375:Aging; D000818:Animals; D004353:Drug Evaluation, Preclinical; D004797:Enzyme-Linked Immunosorbent Assay; D006023:Glycoproteins / Q000032:analysis; D006801:Humans; D051379:Mice; D011818:Rabies / Q000517:prevention & control*; D011819:Rabies Vaccines / Q000032:analysis
chemical_list: D006023:Glycoproteins; D011819:Rabies Vaccines