The 2.0 A X-ray crystal structure of chicken egg white cystatin and its possible mode of interaction with cysteine proteinases.
The crystal structure of chicken egg white cystatin has been solved by X-ray diffraction methods using the multiple isomorphous replacement technique. Its structure has been refined to a crystallographic R value of 0.19 using X-ray data between 6 and 2.0A. The molecule consists mainly of a straight five-turn alpha-helix, a five-stranded antiparallel beta-pleated sheet which is twisted and wrapped around the alpha-helix and an appending segment of partially alpha-helical geometry. The 'highly conserved' region from Gln53I to Gly57I implicated with binding to cysteine proteinases folds into a tight beta-hairpin loop which on opposite sides is flanked by the amino-terminal segment and by a second hairpin loop made up of the similarly conserved segment Pro103I - Trp104I. These loops and the amino-terminal Gly9I - Ala10I form a wedge-shaped 'edge' which is quite complementary to the 'active site cleft' of papain. Docking experiments suggest a unique model for the interaction of cystatin and papain: according to it both hairpin loops of cystatin make major binding interactions with the highly conserved residues Gly23, Gln19, Trp177 and Ala136 of papain in the neighbourhood of the reactive site Cys25; the amino-terminal segment Gly9I - Ala10I of bound cystatin is directed towards the substrate subsite S2, but in an inappropriate conformation and too far away to be attacked by the reactive site Cys25. As a consequence, the mechanism of the interaction between cysteine proteinases and their cystatin-like inhibitors seems to be fundamentally different from the 'standard mechanism' defined for serine proteinases and most of their protein inhibitors.
Bode
W
W
Max-Planck-Institut für Biochemie, Martinsried, FRG.
Engh
R
R
Musil
D
D
Thiele
U
U
Huber
R
R
Karshikov
A
A
Brzin
J
J
Kos
J
J
Turk
V
V
D000818:Animals; D002645:Chickens; D003460:Crystallization; D015891:Cystatins; D003546:Cysteine Endopeptidases / Q000378:metabolism*; D015853:Cysteine Proteinase Inhibitors; D010206:Papain / Q000378:metabolism*; D010455:Peptides; D011480:Protease Inhibitors / Q000032:analysis* / Q000378:metabolism; D011487:Protein Conformation; D011506:Proteins / Q000032:analysis* / Q000378:metabolism; D012984:Software; D014961:X-Ray Diffraction
D015891:Cystatins; D015853:Cysteine Proteinase Inhibitors; D010455:Peptides; D011480:Protease Inhibitors; D011506:Proteins; C100158:cystatin, egg-white; D003546:Cysteine Endopeptidases; D010206:Papain