Response of nasal ciliated cells of the guinea-pig during allergic reactions in human blood.
Nasal mucosal samples from thirty-five guinea-pigs were placed in a chamber, containing the medium RPMI 1640. Ciliary activity (beats.s-1) of the most active cell in each mucosal culture was measured using a photoelectric method. The RPMI 1640 was then replaced by 2 ml of RPMI 1640 and 1 ml of heparinized human blood from a non-allergic patient, with a ragweed-sensitive nasal allergy, or patients with D. farinae-sensitive nasal allergy: the ciliary activity of the same ciliated cell in each culture did not change significantly. We then added 1 micrograms of D. farinae extracts to evoke an in vitro allergic reaction. Ciliary excitation was induced when D. farinae extracts were added to the blood from D. farinae-sensitive subjects but not when added to blood from subjects without sensitivity to D. farinae. The peak and plateau of such an event occurred after 20-30 min. Ciliary stimulations were correlated with D. farinae-induced histamine release from whole blood. Thus, in vitro allergic reactions stimulate activity in cilia from normal nasal mucosa.
Ohashi
Y
Y
Department of Otolaryngology, Osaka City University Medical School, Japan.
Nakai
Y
Y
Ikeoka
H
H
Koshimo
H
H
Esaki
Y
Y
D000485:Allergens; D000818:Animals; D002923:Cilia / Q000502:physiology; D003470:Culture Media; D006168:Guinea Pigs; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008925:Mites / Q000276:immunology; D009079:Mucociliary Clearance; D009297:Nasal Mucosa / Q000166:cytology*; D011058:Pollen / Q000276:immunology; D012130:Respiratory Hypersensitivity / Q000097:blood*
D000485:Allergens; D003470:Culture Media