[Synthesis of new terminator substrates for DNA-polymerases-- nucleoside-5'-triphosphates with modified carbohydrate residue].
Synthesis of 3'-chloro- and 3'-cyanothio-2',3'-dideoxythymidine by the reaction of 2,3'-anhydro-2'-deoxythymidine with ammonium chloride and lithium thiocyanate, respectively, has been developed. In addition, 3'-methanesulphonylamido- and 3'-sulphonylamido-2',3'-dideoxythymidines were synthesized starting from 3'-amino-2',3'-dideoxythymidine. All these compounds along with 2',3'-anhydroriboadenosine,2',3'-anhydrolyxoadenosine, 2',3'-O-isopropylidenecytidine, and 2,3'-anhydro-2'-deoxythymidine were transformed into 5'-triphosphates by treatment with phosphoryl tris-1,2,4-triazolide and then with bis(tri-n-butylammonium)pyrophosphate. All 5'-triphosphates of nucleoside analogues were tested as termination substrates in cell-free systems with various DNA polymerases.
Diatkina
N B
NB
Atrazheva
E D
ED
Aleksandrova
L A
LA
Kraevskiĭ
A A
AA
von Janta-Lipinski
M
M
D000818:Animals; D002241:Carbohydrates; D002384:Catalysis; D002417:Cattle; D002474:Cell-Free System; D055598:Chemical Phenomena; D002621:Chemistry; D004259:DNA-Directed DNA Polymerase; D009711:Nucleotides / Q000138:chemical synthesis*; D013379:Substrate Specificity; D013950:Thymus Gland / Q000201:enzymology
D002241:Carbohydrates; D009711:Nucleotides; D004259:DNA-Directed DNA Polymerase
Sintez novykh terminatornykh substratov DNK-polimeraz-- nukleozid-5'-trifosfatov s modifitsirovannym uglevodnym ostatkom.