Lymph esterases of the house mouse (Mus musculus)--II. The role of esterase-2 in fat resorption.
1. Intralipid infusion into the duodenum of Mus musculus was accompanied by changes in lymph and serum concentrations of two esterase isozymes, ES-1 and ES-2. Whereas ES-1 levels declined in both lymph and serum, ES-2 levels increased 5-fold in lymph within 120 min, and fell to a plateau 3- to 4-fold the fasting level; serum levels of ES-2 increased continually. 2. The changes in lymph ES-2 concentrations were paralleled by lymph triglyceride concentration during Intralipid infusion. Genetically determined differences in the concentration of two allozymes, ES-2B and ES-2D, were reflected in differences in lymph triglyceride levels. The lymph triglyceride concentration was strongly correlated with approximately the cube root of the lymph ES-2 concentration for both allozymes. 3. The source of lymph ES-2 during fat resorption was probably an intracellular jejunal pool; serum ES-2 also re-entered the lymph but this fraction was not influenced by fat resorption. 4. Purified chylomicrons possessed no esterase activity; however, it was postulated that ES-2 plays an essential role in fat resorption and is extruded with the primary chylomicrons from the enterocyte.
Wassmer
B
B
Abteilung für Chemische Pathologie, Universität Freiburg, FRG.
Augenstein
U
U
Ronai
A
A
de Looze
S
S
von Deimling
O
O
D000818:Animals; D043182:Carboxylesterase; D002265:Carboxylic Ester Hydrolases / Q000037:antagonists & inhibitors / Q000097:blood / Q000378:metabolism*; D002914:Chylomicrons / Q000378:metabolism; D005217:Fat Emulsions, Intravenous / Q000008:administration & dosage; D007527:Isoenzymes / Q000037:antagonists & inhibitors / Q000097:blood / Q000378:metabolism*; D050356:Lipid Metabolism; D008196:Lymph / Q000201:enzymology*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009596:Nitrophenols / Q000494:pharmacology; D014280:Triglycerides / Q000378:metabolism
D002914:Chylomicrons; D005217:Fat Emulsions, Intravenous; D007527:Isoenzymes; D009596:Nitrophenols; D014280:Triglycerides; C002887:bis(4-nitrophenyl)phosphate; D002265:Carboxylic Ester Hydrolases; D043182:Carboxylesterase