title: Human serum antibodies reactive with dietary proteins. IgG subclass distribution.
abstract: The isotype distribution of human IgG antibodies reactive with common dietary proteins has been evaluated in sera from adult patients with the irritable bowel syndrome and with bronchial asthma using a solid-phase immunoassay (ELISA). In both these medical disorders, serum antibodies reactive with ovalbumin or gliadin were restricted predominantly to the IgG4 isotype; however, IgG antibodies reactive with bovine milk antigens, notably casein, were often restricted to both the IgG2 and IgG4 isotypes. A similar serum IgG antibody isotype distribution for these dietary protein antigens was also demonstrated in IgG antibody-positive healthy adults. These data amplify the view that production of antibodies of the IgG4 isotype may reflect a normal immune response to dietary protein antigens presented at mucosal surfaces.
authors.affiliation: University Department of Immunology, Royal Liverpool Hospital, UK.
authors.forename: R M
authors.initials: RM
authors.lastname: Barnes
authors.forename: P M
authors.initials: PM
authors.lastname: Johnson
authors.forename: M M
authors.initials: MM
authors.lastname: Harvey
authors.forename: J
authors.initials: J
authors.lastname: Blears
authors.forename: R
authors.initials: R
authors.lastname: Finn
mesh_terms: D000328:Adult; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D001249:Asthma / Q000276:immunology; D003109:Colonic Diseases, Functional / Q000276:immunology; D004044:Dietary Proteins / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis / Q000145:classification*; D007132:Immunoglobulin Isotypes / Q000032:analysis; D008894:Milk Proteins / Q000276:immunology
chemical_list: D004044:Dietary Proteins; D007074:Immunoglobulin G; D007132:Immunoglobulin Isotypes; D008894:Milk Proteins