title: The complete primary structure of the cellular retinaldehyde-binding protein from bovine retina.
abstract: Cellular retinaldehyde-binding protein (CRALBP) carries 11-cis-retinol and 11-cis-retinaldehyde as endogenous ligands and may be a functional component of the visual cycle. The complete amino acid sequence of CRALBP from bovine retina has been determined by direct microanalysis of the protein. Bovine CRALBP contains 316 residues in a single amino-terminal-blocked chain corresponding to a molecular weight of 36,421, inclusive of the blocking group. Overlapping peptides were generated by cleavage of lysyl, arginyl, methionyl, glutamyl, and one tryptophanyl bond and sequenced by gas-phase Edman degradation. Analysis of amino-terminal arginyl and methionyl peptides by fast atom bombardment mass spectrometry identified the N alpha-blocking group as an acetyl moiety, and tandem mass spectrometry provided the sequence of the first 9 residues. Comparison of CRALBP with other known protein sequences reveals no significant structural relatedness. The present results provide a basis for relating CRALBP domains with physiological function and for the future development of a more detailed three-dimensional model of the interaction of 11-cis-retinaldehyde with protein.
authors.affiliation: W. Alton Jones Cell Science Center, Lake Placid, New York 12946.
authors.forename: J W
authors.initials: JW
authors.lastname: Crabb
authors.forename: C M
authors.initials: CM
authors.lastname: Johnson
authors.forename: S A
authors.initials: SA
authors.lastname: Carr
authors.forename: L G
authors.initials: LG
authors.lastname: Armes
authors.forename: J C
authors.initials: JC
authors.lastname: Saari
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002352:Carrier Proteins / Q000032:analysis*; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D013058:Mass Spectrometry; D008970:Molecular Weight; D012160:Retina / Q000032:analysis*; D012172:Retinaldehyde / Q000378:metabolism
chemical_list: C020932:11-cis-retinal-binding protein; D002352:Carrier Proteins; D012172:Retinaldehyde