Dissimilar plasmids isolated from Pseudomonas diminuta MG and a Flavobacterium sp. (ATCC 27551) contain identical opd genes.
The opd (organophosphate-degrading) gene derived from a 43-kilobase-pair plasmid (pSM55) of a Flavobacterium sp. (ATCC 27551) has a sequence identical to that of the plasmid-borne gene of Pseudomonas diminuta. Hybridization studies with DNA fragments obtained by restriction endonuclease digestion of plasmid DNAs demonstrated that the identical opd sequences were encoded on dissimilar plasmids from the two sources.
Harper
L L
LL
Department of Biochemistry and Biophysics, Texas A&M University System, College Station 77843.
McDaniel
C S
CS
Miller
C E
CE
Wild
J R
JR
D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D015342:DNA Probes; D004269:DNA, Bacterial / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D005417:Flavobacterium / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D009943:Organophosphorus Compounds / Q000378:metabolism*; D010957:Plasmids; D011549:Pseudomonas / Q000235:genetics* / Q000378:metabolism; D012689:Sequence Homology, Nucleic Acid; D012988:Soil Microbiology
D015342:DNA Probes; D004269:DNA, Bacterial; D009943:Organophosphorus Compounds