Effects of estrogen-induced hyperlipidemia on the erythrocyte membrane in chicks.
The effects of estrogen-induced hyperlipidemia on plasma lipid peroxidation, fatty acid composition and osmotic fragility of erythrocytes in chickens were studied. Young male chickens implanted with estrogen for three wk developed a marked hyperlipidemia. Plasma levels of triglyceride, cholesterol and phospholipid were elevated 68-, four- and 24-fold, respectively, over controls. There was also a two-fold increase in plasma lipid peroxidation measured by the thiobarbituric acid test. Vitamin E supplement (1,000 IU/kg diet) reduced the plasma lipid peroxidation to the control level, but had no effect on the plasma lipid content. Estrogen-induced hyperlipidemia resulted in changes in the fatty acid composition of membrane lipids of erythrocytes. The major changes were an increase in oleic acid from 10.0% to 14.2% and a decrease in linoleic acid from 31.3% to 26.0%. The erythrocytes with an altered membrane fatty acid composition were found to have an increased osmotic fragility. It was apparent that there was a direct correlation between the oleic acid content and the osmotic fragility of erythrocytes.
Cho
B H
BH
Harlan E. Moore Heart Research Foundation, Champaign, IL 61820.
Smith
T L
TL
Park
J R
JR
Kummerow
F A
FA
D000818:Animals; D002645:Chickens; D004910:Erythrocyte Membrane / Q000032:analysis*; D004967:Estrogens / Q000494:pharmacology*; D005227:Fatty Acids / Q000097:blood; D006461:Hemolysis; D006949:Hyperlipidemias / Q000097:blood* / Q000139:chemically induced; D008054:Lipid Peroxides / Q000097:blood; D008297:Male; D008563:Membrane Lipids / Q000032:analysis; D009996:Osmotic Fragility
D004967:Estrogens; D005227:Fatty Acids; D008054:Lipid Peroxides; D008563:Membrane Lipids