title: Role of microfilaments in the exocytosis of rat peritoneal mast cells.
abstract: When rat peritoneal mast cells were treated with the potent histamine releaser compound 48/80 in the presence of tetramethylrhodamine-labeled G-actin, the fluorescent G-actin particles were bound to the surface of extruded granules and to the cell surface. When rhodamine-phalloidin was incorporated into permeabilized rat mast cells in a Ca2+-free medium, rhodamine fluorescence was observed on the cell surface accompanied by serpentine ridges which appeared in the resting state. After perfusion with a cytosol-like solution containing Ca2+, rhodamine fluorescence appeared on the cell surface as a distinct network formation. In some cases, circular fluorescences which appeared to surround the extruded pores were observed in the cell membrane. These findings indicate the existence of actin filaments in the cell membrane and/or subplasmalemmal network. In whole-mount preparations, the granules were surrounded very densely with microfilaments of various widths. After exposure to compound 48/80, granules protruding through the cell membrane were wrapped in many filaments. The extruded granules located in the periphery of the cells were connected by many filamentous structures and disruptions in the middle of these connections were occasionally observed. In some cases, circular configurations of microfilaments were observed at the bottom of the extruded granules and in others dense gatherings of microfilaments were seen just beneath the granules, as if the latter were being pushed up and out of the cells.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Pharmacology, Faculty of Pharmaceutical Sciences, Okayama University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Tasaka
authors.forename: M
authors.initials: M
authors.lastname: Akagi
authors.forename: K
authors.initials: K
authors.lastname: Miyoshi
authors.forename: M
authors.initials: M
authors.lastname: Mio
mesh_terms: D008841:Actin Cytoskeleton / Q000502:physiology* / Q000648:ultrastructure; D000199:Actins / Q000032:analysis; D000818:Animals; D003599:Cytoskeleton / Q000502:physiology*; D005089:Exocytosis; D005456:Fluorescent Dyes; D046911:Macromolecular Substances; D008297:Male; D008407:Mast Cells / Q000502:physiology* / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D010529:Peritoneal Cavity; D010590:Phalloidine; D051381:Rats; D011919:Rats, Inbred Strains; D012235:Rhodamines; D003189:p-Methoxy-N-methylphenethylamine
chemical_list: D000199:Actins; D005456:Fluorescent Dyes; D046911:Macromolecular Substances; D012235:Rhodamines; D010590:Phalloidine; D003189:p-Methoxy-N-methylphenethylamine; C005358:tetramethylrhodamine