title: Monoclonal antibodies to dalargin--a synthetic analog of enkephalins.
abstract: Monoclonal antibodies (MABs) to a new drug Dalargin (Tyr-D-Ala-Gly-Phe-Leu-Arg) enhancing ulcer healing have been produced. Dalargin is a synthetic analog of Leu-enkephalin. With about 40 compounds tested in competition radioimmunoassay it has been shown that specificity of the MABs is directed against the N-terminal tetrapeptide of the molecule. The MABs are sensitive to amino acid substitutions in any of the positions of the fragment and have no cross-reactivity with endogenous opioids. Their further application in pharmacokinetic studies in humans and for characterization of opioid receptors is discussed.
authors.affiliation: National Cardiology Research Center, Academy of Medical Sciences, Moscow, U.S.S.R.
authors.forename: N M
authors.initials: NM
authors.lastname: Ananyeva
authors.forename: V A
authors.initials: VA
authors.lastname: Tischenko
authors.forename: J D
authors.initials: JD
authors.lastname: Bespalova
authors.forename: B L
authors.initials: BL
authors.lastname: Pekelis
authors.forename: L Z
authors.initials: LZ
authors.lastname: Yakubov
authors.forename: V A
authors.initials: VA
authors.lastname: Vinogradov
authors.forename: O V
authors.initials: OV
authors.lastname: Rokhlin
authors.forename: M I
authors.initials: MI
authors.lastname: Titov
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D004743:Enkephalin, Leucine / Q000031:analogs & derivatives* / Q000276:immunology; D016308:Enkephalin, Leucine-2-Alanine / Q000031:analogs & derivatives*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D010446:Peptide Fragments; D004743:Enkephalin, Leucine; D016308:Enkephalin, Leucine-2-Alanine; C040996:enkephalin-Leu, Ala(2)-Arg(6)-