title: A DNA-modification methylase from Bacillus stearothermophilus V.
abstract: A type II modification methylase (M BstVI) was partially purified from the thermophilic bacterium Bacillus stearothermophilus V. The methylase catalyses the transfer of methyl groups from S-adenosyl-L-methionine to unmodified double-stranded DNA. The product of methylation was identified by paper chromatography as N6-methyladenine. Since M BstVI protects DNA against cleavage by BstVI and XhoI restriction endonucleases, it follows that it methylates the adenine residue in the sequence 5'-C-T-C-G-A-G-3'.
authors.affiliation: Laboratorio de Biologia Molecular, Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Chile, Santiago.
authors.forename: R
authors.initials: R
authors.lastname: Barra
authors.forename: M
authors.initials: M
authors.lastname: Chiong
authors.forename: E
authors.initials: E
authors.lastname: González
authors.forename: C
authors.initials: C
authors.lastname: Vásquez
mesh_terms: D004247:DNA / Q000378:metabolism; D001411:Geobacillus stearothermophilus / Q000201:enzymology* / Q000254:growth & development; D007700:Kinetics; D008745:Methylation; D009994:Osmolar Concentration; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific) / Q000302:isolation & purification / Q000378:metabolism*; D013696:Temperature
chemical_list: D004247:DNA; C057988:DNA modification methylase BstVI; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific)