title: The use of specimen tilt in transmission electron microscopy of the central nervous system.
abstract: Thin sections of nervous tissue were viewed at different tilt angles using a transmission electron microscope equipped with a eucentric goniometer stage. In a comparison study of various degrees of tilt, one can observe additional morphological features within synaptic profiles, define subsynaptic structures such as Taxi-bodies, and clearly see the crystalline formation of cytochemical tracers. This study demonstrates the value of tilting thin-sections in the analysis of synapses and other biological material at the ultrastructural level.
authors.affiliation: Department of Anatomy, University of California, San Francisco 94143.
authors.forename: A M
authors.initials: AM
authors.lastname: Milroy
authors.forename: D D
authors.initials: DD
authors.lastname: Ralston
mesh_terms: D000818:Animals; D001921:Brain / Q000648:ultrastructure*; D002415:Cats; D008253:Macaca mulatta; D008854:Microscopy, Electron; D013048:Specimen Handling / Q000379:methods*; D013569:Synapses / Q000648:ultrastructure