Characterization of an intronless human calmodulin-like pseudogene.
We report the isolation and characterization of a human genomic clone encoding a calmodulin-like pseudogene. It contains an open reading frame of 444 nucleotides, not interrupted by introns. The nucleotide sequence of the open reading frame shows 80%, 71% and 69% identity to the previously reported human calmodulin cDNAs lambda ht6 [17], hCWP [22], and lambda hCE1 [23], respectively. The derived amino acid sequence has only 85% identity to vertebrate calmodulin, but shows four potentially functional Ca2+-binding loops. In the human tissues tested, this pseudogene is not expressed, though gene structure including promoter elements and a putative polyadenylation site seems to be intact.
Koller
M
M
Laboratory for Biochemistry, Swiss Federal Institute of Technology (ETH), Zürich.
Strehler
E E
EE
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002147:Calmodulin / Q000235:genetics*; D003001:Cloning, Molecular; D005796:Genes; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D011544:Pseudogenes; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D002147:Calmodulin