Effect of time on measurement of hepatic glucose production.
Basal hepatic glucose production (HGP) was determined in obese and nonobese normal subjects and patients with noninsulin-dependent diabetes mellitus (NIDDM) using [3-3H]glucose and the nonsteady state equations of Steele. When HGP was estimated at hourly intervals from 0800-1300 h, it became evident that calculated values for HGP fell for the first 2-4 h until a plateau was reached, and this decline was quite precipitous during the first 2 h in patients with NIDDM. Furthermore, when the same patient with NIDDM was studied on two occasions, similar values for HGP were not uniformly obtained unless measurements were made at least 4 h after [3-3H] glucose administration. Since it has been the convention to use the nonsteady state equations of Steele to calculate HGP in patients with NIDDM 2 h after [3-3H] glucose administration, it is almost certain that published values for HGP in patients with NIDDM are falsely high. Based upon the data presented, we suggest that HGP using [3-3H]glucose and the nonsteady state Steele equations be measured for at least a 4-h period in patients with NIDDM in order to increase the validity of the calculated value.
Chen
Y D
YD
Department of Medicine, Stanford University School of Medicine, California.
Swislocki
A L
AL
Jeng
C Y
CY
Juang
J H
JH
Reaven
G M
GM
D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism; D005947:Glucose / Q000096:biosynthesis*; D006801:Humans; D008099:Liver / Q000378:metabolism*; D009765:Obesity / Q000378:metabolism; D013997:Time Factors
D005947:Glucose