title: Effects of gentamicin and monomer on bone. An in vitro study.
abstract: Gentamicin-loaded bone cement is used with increasing frequency in primary and revision arthroplasty. Considering the high local concentration and the well-known toxic effect of gentamicin on the kidney, a similar inhibiting effect on bone tissue might be expected. In a series of in vitro studies using paired mouse calvaries cultured for 2 days, the authors found a dose-dependent decrease in the release of previously incorporated calcium-45 (45Ca) or tritiated proline and a decrease in alkaline phosphatase activity. In combination with methylmethacrylate, a small additional reduction in 45Ca release and a marked decrease in alkaline phosphatase activity were recorded. These results indicate that released gentamicin and monomer from antibiotic-supplemented bone cement depresses bone turnover and might thus play an important part in the pathogenesis of loosening.
authors.affiliation: Department of Orthopedic Surgery, University Hospital of Copenhagen, Denmark.
authors.forename: J G
authors.initials: JG
authors.lastname: Pedersen
authors.forename: B
authors.initials: B
authors.lastname: Lund
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001842:Bone and Bones / Q000187:drug effects*; D002132:Calcium Radioisotopes; D003864:Depression, Chemical; D004305:Dose-Response Relationship, Drug; D005839:Gentamicins / Q000633:toxicity*; D066298:In Vitro Techniques; D008768:Methylmethacrylates / Q000633:toxicity*; D051379:Mice; D010006:Osteoblasts / Q000187:drug effects; D010010:Osteoclasts / Q000187:drug effects; D011392:Proline; D014316:Tritium
chemical_list: D002132:Calcium Radioisotopes; D005839:Gentamicins; D008768:Methylmethacrylates; C030159:gentamicin-polymethylmethacrylate bead; D014316:Tritium; D011392:Proline; D000469:Alkaline Phosphatase