Recombinant human tumor necrosis factor increases granulocyte cell-surface complement receptor number.
Exposure of human buffy-coat granulocytes to tumor necrosis factor (TNF) produced cellular activation as indicated by an increase in the neutrophil and monocyte cell-surface number of C3b receptors (measured by indirect immunofluorescence). The degree of receptor increase depended on the dose of TNF from 25 to 1250 pg/mL. Results of kinetic analysis confirmed this response: TNF, 250 pg/mL, caused an increase in the C3b receptor number within ten minutes. Purified neutrophils exhibited a similar increase in cell-surface C3b receptors dependent on the concentration of TNF. Tumor necrosis factor could be a mediator of granulocyte activation in patients.
Reed
D
D
Department of Surgery, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115.
Moore
F D
FD
D006098:Granulocytes / Q000378:metabolism*; D006801:Humans; D008565:Membrane Proteins / Q000276:immunology / Q000378:metabolism; D009000:Monocytes / Q000378:metabolism; D009504:Neutrophils / Q000276:immunology / Q000378:metabolism; D011951:Receptors, Complement / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D008565:Membrane Proteins; D011951:Receptors, Complement; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha