An immunoassay for monitoring human exposure to ethylene oxide.
A novel immunochemical approach has been developed to monitor human exposures to ethylene oxide (EO). The method exploits the interaction of EO with the amino function of the N-terminal valine residue of the alpha-chain of human haemoglobin (Hb). Antibodies were raised against the adducted valine in the form of the N-terminal tryptic heptapeptide and have been used to develop a sensitive radioimmunoassay (RIA) for the adducted heptapeptide. This method has been fully validated against a gas chromatography-mass spectrometry (GC-MS) method and has been applied to the monitoring of EO exposure in a group of sterilization workers.
Wraith
M J
MJ
Shell Research Ltd., Sittingbourne Research Centre, Kent, UK.
Watson
W P
WP
Eadsforth
C V
CV
van Sittert
N J
NJ
Törnqvist
M
M
Wright
A S
AS
D004784:Environmental Monitoring / Q000379:methods*; D005027:Ethylene Oxide / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D006454:Hemoglobins / Q000378:metabolism*; D006801:Humans; D011863:Radioimmunoassay
D006454:Hemoglobins; D005027:Ethylene Oxide