Converting a eukaryotic transcriptional inhibitor into an activator.
GAL80, an inhibitor of the yeast transcriptional activator GAL4, is converted into an activator by inserting an acidic activating sequence into it. This hybrid activator does not bind to DNA directly, but is brought to DNA by interacting with a derivative of GAL4 that interacts with both DNA and GAL80.
Ma
J
J
Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, Massachusetts 02138.
Ptashne
M
M
D004271:DNA, Fungal / Q000378:metabolism; D005690:Galactose / Q000378:metabolism*; D005786:Gene Expression Regulation; D005800:Genes, Fungal; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic; D015003:Yeasts / Q000235:genetics* / Q000378:metabolism
D004271:DNA, Fungal; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors; D005690:Galactose