An amino-terminal fragment of cholecystokinin-58 is present in the gut: evidence for a similar processing site of procholecystokinin in canine gut and brain.
Radioimmunoassays using antibodies specific for the carboxyl terminus of cholecystokinin (CCK) and the midportion of CCK-58 (raised against synthetic canine CCK-33-(1-27] revealed the existence of a CCK fragment in canine gut and brain extracts which lacks the biologically active carboxyl terminal immunoreactivity. This material eluted on Sephadex G-50 gel permeation chromatography in the region of CCK-58, on high-pressure liquid chromatography (HPLC) after CCK-39 and before CCK-58, and on cation-exchange FPLC it eluted after CCK-58. The immunoreactive pattern, the ratio of absorbance at 280-220 nm and the chromatographic elution positions suggest that this large CCK-like molecule represents an amino-terminal fragment of CCK-58. This fragment is present in canine gut and brain. Therefore, a similar processing site of procholecystokinin is suggested in both tissues.
Eysselein
V E
VE
Abteilung für Gastroenterologie, Universitätsklinikum Essen, F.R.G.
Eberlein
G
G
Ho
F J
FJ
Goebell
H
H
Reeve
J R
JR
D000818:Animals; D001665:Binding Sites; D001923:Brain Chemistry; D002766:Cholecystokinin / Q000032:analysis*; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004285:Dogs; D007422:Intestines / Q000032:analysis*; D010446:Peptide Fragments / Q000032:analysis*; D011498:Protein Precursors / Q000032:analysis; D011863:Radioimmunoassay
D010446:Peptide Fragments; D011498:Protein Precursors; C038823:cholecystokinin 58; D002766:Cholecystokinin