title: Discontinuous polyacrylamide gradient agarose gels resolve a wide range of restriction fragments and optimize the efficiency of nucleic acid transfer.
abstract: A vertical electrophoresis procedure utilizing a discontinuous polyacrylamide gradient agarose gel was developed to resolve DNA fragments ranging in size from over 50 kb to less than 300 bp in length. The gel consisted of a polyacrylamide plug at the base of the gel followed by a gradient of agarose ranging from 0.3 to 0.9%. Restriction fragments migrated shorter distances than in a comparable polyacrylamide-0.3% agarose gel, and small fragments were retained. Southern transfer of DNA fragments from the gradient gel onto nitrocellulose was more efficient than transfer of fragments using a nongradient gel.
authors.affiliation: Covalent Technology Corporation, Ann Arbor, Michigan 48106.
authors.forename: C L
authors.initials: CL
authors.lastname: Jones
authors.forename: N J
authors.initials: NJ
authors.lastname: Simpson
authors.forename: V L
authors.initials: VL
authors.lastname: Waryas
authors.forename: M G
authors.initials: MG
authors.lastname: Pappas
mesh_terms: D015152:Blotting, Northern; D015139:Blotting, Southern; D004247:DNA / Q000032:analysis*; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D015183:Restriction Mapping
chemical_list: D004247:DNA