Isolation and characterization of Chlamydomonas reinhardtii mutants with defects in the induction of flagellar protein synthesis after deflagellation.
Amputating the flagella of Chlamydomonas reinhardtii stimulates increased synthesis of many flagellar proteins within 30 min. We have isolated a series of mutants which are defective in this stimulation, taking advantage of the fact that cells which cannot stimulate flagellar protein synthesis cannot regenerate flagella. More than a dozen mutants which have flagella, but cannot regenerate them after amputation, were isolated and studied by in vivo labeling to identify those non-regenerator mutants which were specifically defective in the induction of flagellar protein synthesis. Ten such mutants have been identified, and in each of them flagellar amputation does not stimulate the synthesis of any of the major flagellar proteins. At least four of the mutants display an interesting conditional phenotype. The synthesis of flagellar proteins after deflagellation is defective only in gametic cells; vegetative cells of these mutants are capable of flagellar protein synthesis after flagellar amputation.
Lefebvre
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PA
Department of Genetics and Cell Biology, University of Minnesota, St. Paul 55108.
Barsel
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SE
Wexler
D E
DE
D000671:Amputation, Surgical; D002696:Chlamydomonas / Q000235:genetics* / Q000378:metabolism; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D005407:Flagella / Q000378:metabolism; D005786:Gene Expression Regulation; D009154:Mutation; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics
D011506:Proteins