Serodiagnosis survey of tuberculosis by a new ELISA method.
In a survey study, the authors used a new Elisa test, designed by Anda Biologicals, Strasbourg, France, to detect specific IgG and IgM antibodies against A60 antigen. Blood samples from 53 subjects were tested with this serological method: 22 with no tubercular diseases and 31 affected by different tubercular lesions. The IgM titers were negative in all control group subjects, in two out of fourteen patients with progressive primary tuberculosis, in thirteen out of sixteen with secondary tuberculosis and in one patient with extrapulmonary tuberculosis. The IgG titers were positive (greater than 1.25 Elisa units) in all cases, except one, of progressive primary tuberculosis, in all cases, except two, of secondary tuberculosis and in the patient affected by extrapulmonary tuberculosis. The performance of this method and the overall results indicate its sensitivity and reliability to detect specific mycobacterial antibodies at different stages of the disease.
Gulletta
E
E
Cattedra di Microbiologia, Dipartimento di Biologia e Patologia Cellulare e Molecolare, Napoli, Italy.
Del Pezzo
M
M
Sanduzzi
A
A
Bariffi
F
F
Covelli
I
I
D000293:Adolescent; D000328:Adult; D000368:Aged; D003625:Data Collection; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis*; D007075:Immunoglobulin M / Q000032:analysis*; D008297:Male; D008875:Middle Aged; D014376:Tuberculosis / Q000175:diagnosis*
D007074:Immunoglobulin G; D007075:Immunoglobulin M