title: Nucleoside analysis of DNA from Trypanosoma brucei and Trypanosoma equiperdum.
abstract: We have digested trypanosome DNA with a combination of pancreatic DNase I, nuclease P1 and bovine alkaline phosphatase and fractionated the resulting nucleosides on a Supelcosil LC-18-S column by high pressure liquid chromatography. We find less than 0.1% unusual nucleosides, both in Trypanosoma brucei and in a Trypanosoma equiperdum stock, in contrast to a previous report of an unusual nucleoside replacing dC at 1.3% of total nucleosides in T. equiperdum. Our results agree with previous suggestions that the modification of inactive telomeric expression sites for variant-specific surface glycoprotein genes in T. brucei only affects a very small fraction of the total DNA.
authors.affiliation: Division of Molecular Biology, The Netherlands Cancer Institute, Amsterdam.
authors.forename: M
authors.initials: M
authors.lastname: Crozatier
authors.forename: R J
authors.initials: RJ
authors.lastname: De Brij
authors.forename: L
authors.initials: L
authors.lastname: Den Engelse
authors.forename: P J
authors.initials: PJ
authors.lastname: Johnson
authors.forename: P
authors.initials: P
authors.lastname: Borst
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000032:analysis* / Q000378:metabolism; D006868:Hydrolysis; D009705:Nucleosides / Q000032:analysis*; D014345:Trypanosoma / Q000235:genetics*; D014346:Trypanosoma brucei brucei / Q000235:genetics*
chemical_list: D009705:Nucleosides; D004247:DNA