Expression and regulation of c-erb-A mRNA from lymphocytes in patients with thyroid dysfunction.
To investigate the expression and the regulation of nuclear triiodothyronine (T3) receptor at the gene level, cellular(c)-erb-A mRNA isolated from lymphocytes in patients with thyroid dysfunction was examined by Northern gel analysis and dot blot hybridization using viral (v)-erb-A cDNA probe. Human lymphocytes contained c-erb-A mRNA (approximately 2.0 kilobase (kb) in length), and c-erb-A mRNA, which was determined by dot blot hybridization, was observed to be increased in hypothyroid patients but unaltered in hyperthyroid patients. The high level of c-erb-A mRNA may contribute in part to the increase in nuclear T3 receptor and these results suggest the presence of up-regulation of nuclear T3 receptor at the gene level in the lymphocytes of hypothyroid patients.
Nagayama
Y
Y
First Department of Internal Medicine, Nagasaki University School of Medicine, Japan.
Yamashita
S
S
Hirayu
H
H
Ashizawa
K
K
Harakawa
S
S
Inoue
S
S
Izumi
M
M
Nagataki
S
S
D002467:Cell Nucleus / Q000032:analysis / Q000648:ultrastructure; D015342:DNA Probes / Q000032:analysis; D004279:DNA, Viral / Q000032:analysis; D005786:Gene Expression Regulation; D006801:Humans; D007037:Hypothyroidism / Q000235:genetics; D008214:Lymphocytes / Q000378:metabolism* / Q000648:ultrastructure; D012333:RNA, Messenger / Q000235:genetics*; D011988:Receptors, Thyroid Hormone / Q000032:analysis / Q000235:genetics; D013961:Thyroid Gland / Q000378:metabolism / Q000503:physiopathology*
D015342:DNA Probes; D004279:DNA, Viral; D012333:RNA, Messenger; D011988:Receptors, Thyroid Hormone