The effect of various antibacterial preparations on the in vitro morphology and chemotactic response of equine neutrophils.
Two independent assay systems were used to study the effect of three antibacterial preparations on in vitro morphology and chemotaxis of equine neutrophils. Incubation of neutrophils with high (200 micrograms/ml) and medium (20 micrograms/ml) concentrations of neomycin impaired their response to standard chemoattractants. Trimethoprim/sulfadoxine (0.4/2.0 micrograms/ml-40/200 micrograms/ml) and benzylpenicillin (0.25-25 micrograms/ml) had no effect. Neutrophils collected from geldings 2 and 24 h after neomycin (5 mg/kg) administration had impaired responses to standard chemoattractants. Benzylpenicillin (13.2 mg/kg) had no effect.
Pycock
J F
JF
Department of Surgery and Obstetrics, Royal Veterinary College, Hawkshead Campus, Hatfield, Hertfordshire, U.K.
Allen
W E
WE
Porter
D J
DJ
Boyd
E H
EH
D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology*; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D004338:Drug Combinations; D006736:Horses / Q000097:blood*; D008297:Male; D009355:Neomycin / Q000494:pharmacology; D009504:Neutrophils / Q000032:analysis / Q000166:cytology / Q000187:drug effects*; D010400:Penicillin G / Q000494:pharmacology; D013413:Sulfadoxine / Q000494:pharmacology; D013997:Time Factors; D014295:Trimethoprim / Q000494:pharmacology
D000890:Anti-Infective Agents; D004338:Drug Combinations; D013413:Sulfadoxine; D014295:Trimethoprim; D009355:Neomycin; D010400:Penicillin G