The effect of bile diversion on satiety and fat absorption from liquid and solid dietary sources.
In previous studies, liquid fat has been used to determine the effect of bile diversion on fat absorption. Since protein digests, in addition to bile salts, are capable of solubilizing lipids, we hypothesized that fat incorporated in the protein-rich matrix of solid food would be less sensitive to bile diversion than fat ingested as an oil or liquid. Using [3H]glycerol triether as a nonabsorbable fat recovery marker, we determined how much [14C]triolein was absorbed from solid (chicken liver) and liquid (margarine) dietary sources. After a standard liquid/solid meal with either the chicken liver or margarine labeled, midintestinal chyme was collected for 6 hr, extracted, and counted for 14C and 3H activity. Zero, eighty, or one hundred percent of endogenous bile was diverted. Fat absorption from both chicken liver and margarine was nearly complete by midintestine with 0% diversion and was little affected by diversion of 80% of bile. Complete biliary diversion significantly decreased fat absorption from margarine (87.9 +/- 4.4 to 37.2 +/- 9.2%, P less than 0.05) but reduced [14C]triolein absorption from chicken liver less consistently and insignificantly (78.8 +/- 6.9 to 43.9 +/- 10.6%). These data indicate that fat absorption is not solely dependent on bile and support the hypothesis that fat ingested in a cellular matrix is less dependent on bile than liquid fat. Using these same animals but with the midintestinal cannulas plugged to expose the distal intestine to unabsorbed luminal nutrients, we also demonstrated that bile diversion of an initial meal reduced food consumption at a meal offered 3 hr later.
Doty
J E
JE
Surgical and Research Service, VAMC, Sepulveda, California.
Gu
Y G
YG
Meyer
J H
JH
D000042:Absorption; D000818:Animals; D001646:Bile / Q000502:physiology*; D002250:Carbon Radioisotopes; D002645:Chickens; D004041:Dietary Fats / Q000493:pharmacokinetics*; D004285:Dogs; D004435:Eating; D007413:Intestinal Mucosa / Q000378:metabolism; D008099:Liver; D008383:Margarine; D012527:Satiation / Q000502:physiology*; D012528:Satiety Response / Q000502:physiology*; D014304:Triolein / Q000493:pharmacokinetics
D002250:Carbon Radioisotopes; D004041:Dietary Fats; D014304:Triolein; D008383:Margarine