[Purification and various properties of uracil-DNA-glycosylase from human placenta].
Uracil-DNA-glycosylase was isolated from human placenta and purified 2100-fold. The apparent Km value for non-methylated DNA substrate of the enzyme is 3.10(-7) M. However, Km for uracil-DNA-glycosylase was 3 times as low when methylated DNA was used as a substrate. It was shown that the initial rate of uracil excision was greater for the non-methylated than for the hypermethylated DNA. The experimental results indicate that the postreplicative methylation of DNA can interfere with uracil excision.
Kuz'min
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IA
Golubovskaia
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VM
Aprelikova
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ON
D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D045647:DNA Glycosylases; D004261:DNA Replication; D004795:Enzyme Stability; D005260:Female; D006801:Humans; D007700:Kinetics; D008745:Methylation; D009699:N-Glycosyl Hydrolases / Q000302:isolation & purification*; D010920:Placenta / Q000201:enzymology*; D011247:Pregnancy; D013379:Substrate Specificity; D051981:Uracil-DNA Glycosidase
D045647:DNA Glycosylases; D009699:N-Glycosyl Hydrolases; D051981:Uracil-DNA Glycosidase
Ochistka i nekotorye svoĭstva uratsil-DNK-glikozilazy iz platsenty cheloveka.