Residual skin injury after repeated irradiation: differences observed using healing, macrocolony, and microcolony endpoints.
Following three repeated tolerance doses to mouse tail skin, residual injury was characterized by a 35% reduction in the iso-effective dose compared to age-matched controls, using healing or macrocolony endpoints. In contrast, the reduction was only 9%, measured using microcolony formation. The colony data showed that the reduction was a constant dose, not a dose-modifying effect. The residual injury is interpreted as due to a reduced density of microcolony-forming cells in the epidermis, and these are less capable of macrocolony formation and hence of re-epithelialization in the repeatedly-irradiated epidermis.
Chen
F D
FD
Department of Radiobiology, Paterson Institute for Cancer Research, Christie Hospital & Holt Radium Institute, Manchester, U.K.
Hendry
J H
JH
D000818:Animals; D003114:Colony-Forming Units Assay; D005260:Female; D051379:Mice; D011829:Radiation Dosage; D011833:Radiation Injuries, Experimental / Q000473:pathology* / Q000503:physiopathology; D012867:Skin / Q000528:radiation effects*; D014945:Wound Healing