Postimplantation embryo culture for the assessment of the teratogenic potential and potency of compounds.
Whole rat embryos cultured during the early stages of organogenesis were subjected to a panel of selected chemicals. Of seventeen known in vivo teratogens, seventeen also induced specific malformations in embryos grown in culture. Of ten chemicals which were reported to be negative in in vivo rat teratogenicity studies, eight also did not provoke dysmorphogenic effects in vitro. Of five additionally tested retinoids, all induced multiple malformations. However, concentrations used to induce these effects varied considerably, isotretinoin inducing malformations at 10(-5) M and arotinoid at 10(-11) M. The results indicate qualitatively as well as quantitatively a high predictability of this in vitro system and suggest that the postimplantation embryo culture system may also be useful in the prospective testing of new drugs and environmental chemicals.
Cicurel
L
L
Department of Toxicology, Nyon, Switzerland.
Schmid
B P
BP
D000014:Abnormalities, Drug-Induced; D000826:Animal Testing Alternatives; D000818:Animals; D000970:Antineoplastic Agents / Q000633:toxicity; D046508:Culture Techniques; D004305:Dose-Response Relationship, Drug; D064420:Drug-Related Side Effects and Adverse Reactions; D004622:Embryo, Mammalian; D051381:Rats; D014801:Vitamin A / Q000031:analogs & derivatives / Q000633:toxicity
D000970:Antineoplastic Agents; D014801:Vitamin A