title: Glucose transporter of the blood-brain barrier and brain in chronic hyperglycemia.
abstract: The effect of chronic hyperglycemia on the glucose transporter moiety of the blood-brain barrier and cerebral cortex was studied in rats 3 weeks after the administration of a single intravenous dose of streptozotocin (60 mg/kg), using specific [3H]cytochalasin B binding methods. Streptozotocin-treated rats developed hyperglycemia, as well as polydipsia and polyuria, and failed to gain weight. The density of D-glucose-displaceable cytochalasin B binding sites in the brain microvessels of streptozotocin-treated hyperglycemic rats was increased by about 30% compared with those of control rats, without change in the affinity of binding. Chronic hyperglycemia had no effect on the density or affinity of specific binding of cytochalasin B to cerebral cortical membranes. These findings do not support the hypothesis that glucose transporters in brain microvessels comprising the blood-brain barrier are "down-regulated" in chronic hyperglycemia.
authors.affiliation: Department of Neurology, University Hospitals of Cleveland, OH 44106.
authors.forename: S I
authors.initials: SI
authors.lastname: Harik
authors.forename: S A
authors.initials: SA
authors.lastname: Gravina
authors.forename: R N
authors.initials: RN
authors.lastname: Kalaria
mesh_terms: D000818:Animals; D001812:Blood-Brain Barrier; D001921:Brain / Q000098:blood supply / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism; D003571:Cytochalasin B / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D008297:Male; D008833:Microcirculation / Q000378:metabolism; D009004:Monosaccharide Transport Proteins / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D009004:Monosaccharide Transport Proteins; D003571:Cytochalasin B