title: Practical hints for peptide sequencing by soft ionization methods.
abstract: Practical hints are summarized for the investigation of peptides by soft ionization methods based on liquid secondary ion mass spectrometry. The method aims: to purify the sample; to force a peptide to the surface by ion pair formation; to recover the sample used for mass spectral analysis; to distinguish between Lys and Gln by derivatization; to spot amino acids in the low mass region; and to improve sequence information by use of peptide fragments, not described previously.
authors.affiliation: Organische Chemie I der Universität Bayreuth, FRG.
authors.forename: W
authors.initials: W
authors.lastname: Kausler
authors.forename: K
authors.initials: K
authors.lastname: Schneider
authors.forename: G
authors.initials: G
authors.lastname: Spiteller
mesh_terms: D000596:Amino Acids / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D013058:Mass Spectrometry; D010446:Peptide Fragments / Q000032:analysis; D010449:Peptide Mapping; D010455:Peptides / Q000032:analysis*
chemical_list: D000596:Amino Acids; D010446:Peptide Fragments; D010455:Peptides