Effect of continuous administration of nicotine on urinary histamine and N tau-methylhistamine levels in the guinea pig.
The effect of continuous subcutaneous administration of S-(-)- and R-(+)-nicotines on urinary excretion levels of histamine and N tau-methylhistamine in guinea pigs, over a 23-day period, has been studied. Urinary levels of these endogenous compounds were measured utilizing paired-ion reversed-phase high performance liquid chromatography with flow-through electrochemical detection. Urinary histamine levels of animals that had been administered either of these nicotine isomers were not significantly different from control values. Initial levels of urinary N tau-methylhistamine (days 2-3) in R-(+)- and S-(-)-nicotine-treated animals were, 2-fold and 8-fold higher, respectively than control levels but in both cases these levels returned to control values over the remainder of the time course examined (days 6-23). These results suggest that exposure to S-(-)-nicotine results in initial histamine release and/or inhibition of histamine uptake. However, longer term exposure to S-(-)-nicotine may not result in significantly altered levels of circulating histamine.
Houdi
A A
AA
College of Pharmacy, University of Kentucky, Lexington 40536.
Godin
C S
CS
Crooks
P A
PA
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D006168:Guinea Pigs; D006632:Histamine / Q000652:urine*; D007279:Injections, Subcutaneous; D008297:Male; D008761:Methylhistamines / Q000652:urine*; D009538:Nicotine / Q000008:administration & dosage / Q000494:pharmacology*; D013237:Stereoisomerism; D014316:Tritium
D008761:Methylhistamines; D014316:Tritium; C031205:3-methylhistamine; D009538:Nicotine; D006632:Histamine