Epidermal growth factor receptor numbers in male and female mouse primary hepatocyte cultures.
Epidermal growth factor receptors (EGF-R) were measured in adult male and female mouse primary hepatocyte cultures. On culture day 1, female hepatocytes had significantly fewer EGF-R than male hepatocytes (1.3 x 10(4) versus 6.2 x 10(5) per cell). Over the next three days, morphological changes consistent with progressive heptocyte dedifferentiation were observed. During this period, EGF-R numbers progressively increased in female cultures and decreased in male cultures, and by day 4 the sexual difference in EGF-R numbers was obliterated. These results indicate that a relationship exists between the degree of differentiation in hepatocyte cultures and the expression of EGF-R on the cell surface.
Benveniste
R
R
Department of Obstetrics and Gynecology, Michael Reese Hospital and Medical Center, Chicago, IL.
Danoff
T M
TM
Ilekis
J
J
Craig
H R
HR
D000818:Animals; D002478:Cells, Cultured; D066246:ErbB Receptors / Q000032:analysis* / Q000378:metabolism; D005260:Female; D008099:Liver / Q000032:analysis* / Q000166:cytology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D012737:Sex Factors
D066246:ErbB Receptors