Development of hamster two-cell embryos in the isolated mouse oviduct in organ culture system.
Hamster early two-cell embryos developed to the expanded blastocyst stage within the isolated mouse ampulla maintained in organ culture system. Mouse ampullae isolated at different times after treating the mice with human chorionic gonadotropin (hCG) (0-72 h) or pregnant mare's serum gonadotropin (PMSG) (30-32 h) were flushed with culture medium, and hamster early two-cell embryos were introduced into these ampullae. Mouse ampullae isolated at 14-32 h after hCG injection were more favorable for the development of the embryos than those isolated at 70-72 h. When mouse ampullae were isolated 30-32 h after hCG or PMSG treatment, 39% of the cultured eggs developed, some of them to the expanded blastocyst stage after additional culture for 65-70 h. These results indicate that unknown oviductal factors stimulate the development of hamster early two-cell embryos, and these factors are under the control of hCG or PMSG. In addition, these factors are common to the mouse and hamster.
Minami
N
N
Department of Animal Science, Kyoto University, Japan.
Bavister
B D
BD
Iritani
A
A
D000818:Animals; D001755:Blastocyst / Q000187:drug effects / Q000502:physiology*; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D006224:Cricetinae; D005187:Fallopian Tubes / Q000502:physiology*; D005260:Female; D006064:Gonadotropins, Equine / Q000494:pharmacology; D051379:Mice; D009028:Morula / Q000502:physiology; D009924:Organ Culture Techniques; D013045:Species Specificity
D006063:Chorionic Gonadotropin; D006064:Gonadotropins, Equine