S-100b protein regulates the activity of skeletal muscle adenylate cyclase in vitro.
We have investigated the effect of the b isoform of S-100 proteins on adenylate cyclase activity of rat skeletal muscle. S-100b inhibits the adenylate cyclase activity in the presence of Mg2+ (5.0-50 mM), while it activates the same enzyme in the presence of Ca2+ (0.1-1.0 mM) dose-dependently in both cases. S-100b counteracts the stimulatory effect of NaF on adenylate cyclase in the presence of Mg2+ and the inhibitory effect of RMI 12330 A in the presence of Ca2+.
Fanò
G
G
Istituto di Biologia Cellulare, Università di Perugia, Italy.
Fulle
S
S
Della Torre
G
G
Giambanco
I
I
Aisa
M C
MC
Donato
R
R
Calissano
P
P
D000067956:Adenylyl Cyclase Inhibitors; D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000502:physiology; D004789:Enzyme Activation; D007097:Imines / Q000494:pharmacology; D008274:Magnesium / Q000502:physiology; D009132:Muscles / Q000201:enzymology*; D051381:Rats; D009418:S100 Proteins / Q000502:physiology*; D012969:Sodium Fluoride / Q000494:pharmacology
D000067956:Adenylyl Cyclase Inhibitors; D007097:Imines; D009418:S100 Proteins; C014925:RMI 12330A; D012969:Sodium Fluoride; D000262:Adenylyl Cyclases; D008274:Magnesium; D002118:Calcium