Recombinant DNA related to hepatitis B and human immunodeficiency viruses in mononuclear cells of patients with AIDS.
Sixty-eight of 73 patients with human immunodeficiency virus (HIV) infection were positive when tested for the presence of hepatitis B virus (HBV)-related DNA sequences in peripheral blood mononuclear cells (PBMCs) by the dot blot method. Twenty-two of the positive DNAs were examined by Southern hybridization and all exhibited a 3.2 kb extrachromosomal DNA fragment that hybridized to HBV DNA. This DNA was isolated from agarose gels and cloned into the EcoRI site of pBR322 DNA. The cloned DNA (pHBI) hybridized to both HBV DNA and HIV cDNA; HBV DNA did not hybridize to HIV cDNA under the same conditions. The results of restriction enzyme analyses indicated that pHBI contains: 1) a large deletion of HBV sequences spanning the 3' end of the HBV surface antigen gene; 2) a small deletion near the 5' end of the HBV core antigen gene; and 3) a region of homology to a one kb central section of the HIV pol gene. These data suggest that the 3.2 kb DNA found in the PBMCs is a natural recombinant between HBV and HIV DNAs raising the possibility not only that this DNA plays a role in the pathogenesis of AIDS but also that other viral recombinant DNAs may be pathogenic in human disease.
Hirschman
S Z
SZ
Department of Medicine, Mount Sinai School of Medicine, City University of New York, New York 10029.
Zucker
M L
ML
D000163:Acquired Immunodeficiency Syndrome / Q000150:complications / Q000382:microbiology*; D015139:Blotting, Southern; D003001:Cloning, Molecular; D015342:DNA Probes; D004279:DNA, Viral / Q000032:analysis* / Q000235:genetics; D006678:HIV / Q000235:genetics*; D006509:Hepatitis B / Q000150:complications / Q000382:microbiology; D006515:Hepatitis B virus / Q000235:genetics*; D006801:Humans; D015151:Immunoblotting; D007963:Leukocytes, Mononuclear / Q000382:microbiology; D009693:Nucleic Acid Hybridization; D011995:Recombination, Genetic
D015342:DNA Probes; D004279:DNA, Viral