Stimulation of prostaglandin E2 biosynthesis by estradiol in rat kidneys.
The effect of estradiol administration on renal prostaglandin (PG) E2 biosynthetic activity in rats was studied. A specific radioimmunoassay for PGE2 was developed and applied in the quantitation of PGE2 biosynthesis in kidney. Conversion of exogenous arachidonic acid into PGE2 by renal microsomal fraction was assayed. Formation of PGE2 was linear in fashion up to 5 min incubation at 37 degrees C, and linear in fashion up to 3.5 mg of microsome used as enzyme source. The renal biosynthesis of PGE2 was significantly increased by estradiol treatment.
Chang
W C
WC
Department of Pharmacology, College of Medicine, National Cheng Kung University, Tainan, Taiwan, Republic of China.
D000818:Animals; D015232:Dinoprostone / Q000096:biosynthesis*; D004958:Estradiol / Q000494:pharmacology*; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D004958:Estradiol; D015232:Dinoprostone