Involvement of serotonin in prolactin release induced by electrical stimulation of the hypothalamus of the turkey (Meleagris gallopavo).
In anesthetized female turkeys electrical stimulation of the ventromedial nucleus (VMN) for 30 min caused increases in plasma prolactin (Prl); with maximum increase above the prestimulation level being 33.6 +/- 5.7 ng/ml for laying hens and 768.1 +/- 187.6 ng/ml for incubating hens. The possibility that serotonin (5-HT) plays a role in electrical stimulation-induced Prl release was investigated after administration of methysergide, a 5-HT receptor blocker (20 mg/kg), and stimulation in either the VMN or the infundibular nuclear complex-median eminence (INF-ME) region. Electrical stimulation in both the VMN and INF-ME region caused increases (P less than 0.05) in plasma Prl. Pretreatment with methysergide prevented the increase in plasma Prl that follows electrical stimulation in the VMN but had no effect on electrical stimulation-induced Prl release in the INF-ME region. We conclude that Prl release in the female turkey requires the functional integrity of serotonergic neurons within the VMN.
el Halawani
M E
ME
Department of Animal Science, University of Minnesota, St. Paul 55108.
Youngren
O M
OM
Silsby
J L
JL
Phillips
R E
RE
D000818:Animals; D001111:Arcuate Nucleus of Hypothalamus / Q000502:physiology; D004558:Electric Stimulation; D005260:Female; D007031:Hypothalamus / Q000502:physiology*; D008473:Median Eminence / Q000502:physiology; D008784:Methysergide / Q000494:pharmacology; D011388:Prolactin / Q000378:metabolism*; D011985:Receptors, Serotonin / Q000187:drug effects / Q000502:physiology; D012701:Serotonin / Q000502:physiology*; D014422:Turkeys / Q000502:physiology*; D014697:Ventromedial Hypothalamic Nucleus / Q000502:physiology
D011985:Receptors, Serotonin; D012701:Serotonin; D011388:Prolactin; D008784:Methysergide