Isolation of monoclonal antibodies to phencyclidine from ascites fluid by preparative isoelectric focusing in the Rotofor.
A monoclonal antibody to phencyclidine was developed, produced in mouse ascites fluid, and purified. The purification used only preparative-scale isoelectric focusing in the Rotofor and dialysis. In 4 h, 25% (4 mg) of the antibody from 10 ml of ascites fluid was purified to homogeneity while 63% of the total antibody was recovered.
Egen
N B
NB
Center for Separation Science, University of Arizona, Tucson 85721.
Bliss
M
M
Mayersohn
M
M
Owens
S M
SM
Arnold
L
L
Bier
M
M
D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D000917:Antibody Formation; D001202:Ascitic Fluid / Q000032:analysis*; D007525:Isoelectric Focusing / Q000295:instrumentation; D051379:Mice; D008810:Mice, Inbred C57BL; D010622:Phencyclidine / Q000031:analogs & derivatives / Q000276:immunology*
D000911:Antibodies, Monoclonal; D010622:Phencyclidine