[Outer membrane proteins in the characterization of salmonellae of various origins].
Outer membrane proteins have been studied in 76 Salmonella strains isolated from various sources and differing in their sensitivity to antibiotics, the presence of R-plasmids and a number of enzymatic properties. The outer membranes have been isolated by the modified method of L. W. Coulton and D. T. F. Wan. The study of the isolated proteins has been carried out by means of gel and disc electrophoresis in polyacrylamide gel with sodium dodecyl sulfate according to K. Weber and M. Osborn. The composition of the proteinograms thus obtained has revealed the presence of essential differences between hospital strains and cultures isolated from animals and from the environment in sporadic infections, as well as between strains belonging to different serovars. The possibility of using the characterization of outer membrane proteins of salmonellae in epidemiological investigations is discussed.
Brusnigina
N F
NF
Volchkevich
Zh A
ZhA
Degteva
G K
GK
Belova
T N
TN
D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis / Q000302:isolation & purification*; D004589:Electrophoresis, Disc; D004591:Electrophoresis, Polyacrylamide Gel; D005516:Food Microbiology; D006801:Humans; D012475:Salmonella / Q000032:analysis / Q000145:classification* / Q000302:isolation & purification; D012703:Serotyping; D014871:Water Microbiology
D001425:Bacterial Outer Membrane Proteins
Belki naruzhnoĭ membrany v kharakteristike sal'monell razlichnogo proiskhozhdeniia.