title: Effects of estradiol on circulating levels of prolactin in female rats bearing ectopic pituitaries.
abstract: The existence of local mechanisms controlling the prolactin (PRL) release from anterior pituitaries (AP) grafted to an ectopic location has been recently described. To study if these mechanisms are affected by estrogens, pituitary-grafted (GRAFT) and sham-operated (SHAM) rats were injected with a single dose of estradiol benzoate (EB), their plasma PRL levels as well as their hypothalamic and AP contents of norepinephrine (NE) and dopamine (DA) being analyzed. Administration of EB to GRAFT animals produced a small increase in their previously high plasma PRL levels, with both an increased NE and a decreased DA content in the ectopic AP. Since NE enhances the PRL release from ectopic AP and DA partially inhibits this secretion these changes may explain such a small increase in PRL levels. However, an additional increase in the decreased PRL release from the in situ AP of these animals cannot be discarded since EB produced also a decrease of the DA content in this tissue with an unaltered hypothalamic content. Finally, administration of this steroid to SHAM animals produced an important increase in plasma PRL levels. Since this increase was correlative to a decrease in DA and NE hypothalamic contents and unaltered AP contents. EB may be supposed to be able to reduce the DA synthesis in the tuberoinfundibular neurons, while the changes in noradrenergic inputs could be more related to the feedback effects of estrogens on the gonadotrophin release.
authors.affiliation: Departamento de Bioquímica, Facultad de Medicina, Universidad Complutense, Madrid, Spain.
authors.forename: J J
authors.initials: JJ
authors.lastname: Fernández-Ruiz
authors.forename: C
authors.initials: C
authors.lastname: Agrasal
authors.forename: M
authors.initials: M
authors.lastname: Cebeira
authors.forename: J A
authors.initials: JA
authors.lastname: Tresguerres
authors.forename: A I
authors.initials: AI
authors.lastname: Esquifino
authors.forename: J A
authors.initials: JA
authors.lastname: Ramos
mesh_terms: D000818:Animals; D004298:Dopamine / Q000097:blood; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D007031:Hypothalamus / Q000378:metabolism; D007668:Kidney; D009638:Norepinephrine / Q000378:metabolism; D010902:Pituitary Gland / Q000637:transplantation*; D011388:Prolactin / Q000097:blood*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D004958:Estradiol; D011388:Prolactin; D004298:Dopamine; D009638:Norepinephrine