[Paracetamol poisoning in a swine model].
In order to prove the function of isolated transplanted hepatocytes in acute liver failure we tried to establish a hepatic failure model in pigs which correlates to a clinical situation. In 12 pigs we administered 500-2000 mg/kg body weight acetaminophen after enzyme induction with Pentobarbital. 7 animals receiving 500-1000 mg/kg acetaminophen survived the intoxication, 5 animals receiving 1000-2000 mg/kg acetaminophen died within 6.5 hours after intoxication because of methemoglobinemia. A close correlation between administered dosage of the drug, acetaminophen blood levels and methemoglobinemia was found. Histology of surviving animals showed a dosage depended cell necrosis. A standardized hepatic failure model could not be established in pigs by acetaminophen intoxication, because dosages of more than 1000 mg/kg were survived. In dosage higher than 1000 mg/kg a side effect of the acetaminophen intoxication the methemoglobinemia limited the life of the animals. Therefore acetaminophen can not be used to induce acute hepatic failure in pigs.
Henne-Bruns
D
D
Chirurgische Universitätsklinik Hamburg.
Artwohl
J
J
Dziwisch
L
L
Kremer
B
B
D000082:Acetaminophen / Q000097:blood / Q000506:poisoning*; D000208:Acute Disease; D000410:Alanine Transaminase / Q000097:blood; D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D056486:Chemical and Drug Induced Liver Injury; D004195:Disease Models, Animal; D008099:Liver / Q000473:pathology / Q000648:ultrastructure; D008107:Liver Diseases / Q000473:pathology*; D008708:Methemoglobinemia / Q000139:chemically induced*; D008854:Microscopy, Electron; D013552:Swine
D000082:Acetaminophen; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D000469:Alkaline Phosphatase
Paracetamol-Intoxikation im Schweinemodell.