Widespread occurrence of calicin, a basic cytoskeletal protein of sperm cells, in diverse mammalian species.
A novel cytoskeletal element consisting of dense webs of thin (3-14 nm) filaments surrounding the nucleus of the sperm head has recently been isolated and shown to be associated with certain major basic proteins. Using antibodies specific for calicin, a prominent Mr-60,000 cytoskeletal protein of the posterior calyx of bull sperm heads detected in immunoblotting on gel electrophoretically separated polypeptides as well as in immunofluorescence and immunoelectron microscopy, we show that the same--or an immunologically related--polypeptide occurs in sperm heads of other species with greatly different morphology, including human, boar, guinea pig, hamster, rat and mouse. The calicin localization in the various species is described and discussed in relation to the specific sperm morphology.
Paranko
J
J
Division of Membrane Biology and Biochemistry, German Cancer Research Center, Heidelberg.
Longo
F
F
Potts
J
J
Krohne
G
G
Franke
W W
WW
D000818:Animals; D006224:Cricetinae; D003598:Cytoskeletal Proteins / Q000378:metabolism*; D006168:Guinea Pigs; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008322:Mammals / Q000378:metabolism*; D051379:Mice; D051381:Rats; D013077:Sperm Head / Q000032:analysis*; D013094:Spermatozoa / Q000032:analysis*; D013552:Swine
C503855:CCIN protein, human; C503856:Ccin protein, mouse; C503857:Ccin protein, rat; D003598:Cytoskeletal Proteins; C057739:calicin