Hepatic gluconeogenesis and mitochondrial function during hibernation.
1. The aim of these studies was to investigate a mitochondrial basis for changes in gluconeogenesis during hibernation. 2. State 3 respiration rates in liver mitochondria from hibernating ground squirrels were reduced by 62-66%. The limiting reaction appeared to be electron transport, particularly in respiratory complex III. 3. The mitochondrial ATP + ADP + AMP content was reduced by 29% during hibernation; cellular adenine nucleotide content was unchanged. 4. Pyruvate carboxylation in intact mitochondria was decreased 75% during hibernation, although total pyruvate carboxylase activity was not lower. 5. Rates of gluconeogenesis in intact hepatocytes isolated from hibernators were lower than in cells from non-hibernators.
Gehnrich
S C
SC
Department of Biology, Tufts University, Medford, MA 02155.
Aprille
J R
JR
D000227:Adenine Nucleotides / Q000378:metabolism; D000818:Animals; D004579:Electron Transport; D005943:Gluconeogenesis; D006605:Hibernation; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism*; D008930:Mitochondria, Liver / Q000378:metabolism; D010101:Oxygen Consumption
D000227:Adenine Nucleotides