title: Immunization procedure-related immunoglobulin levels in the development of antibodies against Cysticercus cellulosae.
abstract: Various immunization procedures were investigated in an effort to improve the number of hybridomas producing antibodies against Cysticercus cellulosae. Ten groups of 5 BALB/c mice were subjected to different immunization procedures and were bled repeatedly over a period of 68 days. The samples of sera thus obtained were tested by enzyme linked immunosorbent assay: total immunoglobulins, IgG and IgM levels were determined. In general, total anticyst antibody titres increased during the course of immunization but in 3 groups the final titre was lower than the maximal antibody titre. Overall, immune tolerance did not appear to be a problem and longer immunization programs seemed to end with slightly higher antibody levels. So far, 4 mice from the group that exhibited the highest immunoglobulin levels have been used for hybridoma production. Out of 124 hybridomas thus obtained, only 1 secreted antibodies against Cysticercus cellulosae.
authors.affiliation: Department of Biology, University of Natal, Durban.
authors.forename: G
authors.initials: G
authors.lastname: Contrafatto
authors.forename: M D
authors.initials: MD
authors.lastname: Pammenter
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis*; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D003552:Cysticercus / Q000276:immunology*; D006825:Hybridomas / Q000276:immunology; D007114:Immunization / Q000379:methods*; D007136:Immunoglobulins / Q000032:analysis*; D051379:Mice; D013621:Taenia / Q000276:immunology*
chemical_list: D000909:Antibodies, Helminth; D000911:Antibodies, Monoclonal; D007136:Immunoglobulins