A novel 190 kDa calmodulin-binding protein associated with brain actomyosin.
We present evidence for a novel calmodulin-binding protein associated with Ca2+-sensitive, non-muscle actomyosin from rabbit brain. Gel filtration chromatography of brain actomyosin and SDS-PAGE analysis of resulting fractions revealed the presence of a stable complex of a 190 kDa polypeptide and calmodulin. Furthermore, brain actomyosin was retained on a column of conjugated calmodulin-Sepharose 4B and eluted by 6 M urea in the presence of Ca2+. Further elution with 6M urea plus EGTA released the 190 kDa polypeptide from the column. These results show that the 190 kDa polypeptide forms a tight complex with calmodulin and that this complex associates with brain actomyosin in a Ca2+-independent manner.
Larson
R E
RE
Departamento de Bioquímica, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Brasil.
Pitta
D E
DE
Ferro
J A
JA
D000205:Actomyosin / Q000032:analysis*; D000818:Animals; D001923:Brain Chemistry; D002148:Calmodulin-Binding Proteins / Q000032:analysis*; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D010446:Peptide Fragments / Q000032:analysis; D011817:Rabbits
D002148:Calmodulin-Binding Proteins; D010446:Peptide Fragments; D000205:Actomyosin