Purification of the fifth component of murine complement from ascites fluid.
The murine complement component C5 was purified on an affinity column using a monoclonal anti-mouse C5 antibody. We describe in this paper that ascites fluid from normal (C5-sufficient) mice contains almost as much C5 protein as mouse serum. Since ascites fluid is much easier to obtain in large quantities it is a convenient source for the purification of this mouse serum protein.
Lin
R H
RH
Basel Institute for Immunology, Switzerland.
Stockinger
B
B
D000818:Animals; D000911:Antibodies, Monoclonal; D001202:Ascitic Fluid / Q000032:analysis*; D002846:Chromatography, Affinity; D003182:Complement C5 / Q000276:immunology / Q000302:isolation & purification* / Q000502:physiology; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006461:Hemolysis; D051379:Mice; D008807:Mice, Inbred BALB C
D000911:Antibodies, Monoclonal; D003182:Complement C5