[Isolation of influenza virus hemagglutinin and its separation into subunits by a stage-by-stage scheme for viral protein fractionation].
The virus envelope protein, hemagglutinin, recovered by DTAB extraction of influenza virions, was purified to a high degree by the gel filtration high-performance liquid chromatography (GF HPLC). Then hemagglutinin was reduced and alkylated with iodoacetamide and the subunits were resolved by GF HPLC on the columns Spherogel TSK G 2000 SW and 3000 SW (600 X 7.5 mm ID).
Glushakova
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Votiakov
V I
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Ksenofontov
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D002850:Chromatography, Gel / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006023:Glycoproteins / Q000302:isolation & purification; D006389:Hemagglutinins, Viral / Q000302:isolation & purification*; D009980:Influenza A virus / Q000302:isolation & purification*; D014764:Viral Proteins / Q000302:isolation & purification*
D006023:Glycoproteins; D006389:Hemagglutinins, Viral; D014764:Viral Proteins
Vydelenie gemaggliutinina virusa grippa i razdelenie ego na sub''edinits poétapnoĭ skhemoĭ fraktsionirovaniia virusnykh belkov.