title: NH4+ ion-selective microelectrode based on the antibiotics nonactin/monactin.
abstract: A liquid-membrane microelectrode (less than or equal to 1 micron tip diameter) using macrotetrolide antibiotics as ion-selective components is described. The electrode shows selectivities of NH4+ over K+, Na+ and H+ of 3.8, 100 and 150, respectively. The stability and reproducibility of the sensor signal and the response time are determined in solutions with a typical intracellular ion background. The microelectrode does not suffer from significant interference by inorganic and organic inhibitors and lipophilic cations, but high concentrations of lipophilic anions may interfere considerably.
authors.affiliation: Department of Organic Chemistry, Swiss Federal Institute of Technology (ETH), Zürich.
authors.forename: T
authors.initials: T
authors.lastname: Bührer
authors.forename: H
authors.initials: H
authors.lastname: Peter
authors.forename: W
authors.initials: W
authors.lastname: Simon
mesh_terms: D000900:Anti-Bacterial Agents; D004594:Electrophysiology / Q000295:instrumentation*; D066298:In Vitro Techniques; D018942:Macrolides; D008839:Microelectrodes; D000644:Quaternary Ammonium Compounds
chemical_list: D000900:Anti-Bacterial Agents; D018942:Macrolides; D000644:Quaternary Ammonium Compounds; C100287:monactin; C050476:nonactin