title: Evaluation of a fluorescence polarization immunoassay procedure for quantitation of isepamicin, a new aminoglycoside antibiotic.
abstract: A fluorescence polarization immunoassay (FPIA) procedure for measuring isepamicin, a new aminoglycoside antibiotic, was evaluated. The range of the assay studied was from 0.3-50 micrograms/ml and the calibration curve can be stored for at least 32 days. There was no interference from hemoglobin (up to 400 mg/dl) and bilirubin (up to 20 mg/dl). Within-run precision on each 20 replicates at 5, 15, and 25 micrograms/ml yielded a coefficient of variation (CV) of 1.44-2.52%; between-run precision on analyses at 5, 15, and 25 micrograms/ml over five periods yielded a CV of 1.26-1.59%. Cross-reactivities of the assay system with tobramycin and amikacin were null, but that with gentamicin, of which isepamicin is a derivative, was approximately 14-29%. Data obtained by microbiological assay and FPIA and by high-performance liquid chromatography and FPIA correlated significantly, with coefficients of correlation being 0.980 (n = 70) and 0.965 (n = 39), respectively. The FPIA offers a rapid, efficient, and accurate system for therapeutic monitoring of isepamicin plasma levels.
authors.affiliation: Department of Pharmacology, Hamamatsu University School of Medicine, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Uematsu
authors.forename: A
authors.initials: A
authors.lastname: Mizuno
authors.forename: Y
authors.initials: Y
authors.lastname: Suzuki
authors.forename: R
authors.initials: R
authors.lastname: Sato
authors.forename: T
authors.initials: T
authors.lastname: Yamazaki
authors.forename: M
authors.initials: M
authors.lastname: Nakashima
mesh_terms: D000328:Adult; D000583:Amikacin / Q000008:administration & dosage; D001663:Bilirubin / Q000502:physiology; D001681:Biological Assay; D002851:Chromatography, High Pressure Liquid; D004347:Drug Interactions; D005839:Gentamicins / Q000032:analysis* / Q000493:pharmacokinetics; D006461:Hemolysis; D006801:Humans; D007118:Immunoassay / Q000379:methods; D008297:Male; D015203:Reproducibility of Results; D014031:Tobramycin / Q000008:administration & dosage
chemical_list: D005839:Gentamicins; D000583:Amikacin; C016927:isepamicin; D001663:Bilirubin; D014031:Tobramycin