Inhibition of in vitro amino acylation and translation by adenosine antibodies.
Adenosine antibodies markedly inhibited in vitro amino acylation of tRNA in a dose-dependent manner. The inhibition was specific as it was reversed by the homologous hapten. Addition of excess tRNA reversed the inhibition indicating that binding of antibodies to tRNA is responsible for inhibition. Adenosine antibodies also inhibited in vitro translation of endogenous mRNAs in rabbit reticulocyte lysate in a dose-dependent manner. The homologous hapten reversed the inhibition showing thereby the immunospecificity of inhibition.
Vaishnav
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YN
Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore.
Antony
A
A
D000241:Adenosine / Q000276:immunology*; D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism; D000818:Animals; D000906:Antibodies; D004305:Dose-Response Relationship, Drug; D012346:RNA, Transfer, Amino Acyl / Q000276:immunology*; D051381:Rats; D014158:Transcription, Genetic
D000906:Antibodies; D012346:RNA, Transfer, Amino Acyl; D000604:Amino Acyl-tRNA Synthetases; D000241:Adenosine