Assignment of gene for coagulation factor V to chromosome 1 in man and to chromosome 13 in rat.
Two different factor V cDNA fragments were used as hydridization probes in the chromosomal assignment of the human and rat factor V genes. A 1.6-kb EcoRI fragment was used as a hybridization probe to analyze a panel of human-rodent somatic cell hybrids. Cosegregation of factor V specific DNA restriction fragments with human chromosome 1 was observed. In addition, a panel of rat-mouse somatic cell hybrids was analyzed with another human factor V cDNA probe to localize the gene for rat coagulation factor V. In the rat, the gene for coagulation factor V was found to be located in chromosome 13. This is the first gene in the rat to be localized to chromosome 13.
Dahlbäck
B
B
Department of Clinical Chemistry, University of Lund, Malmö General Hospital, Sweden.
Hansson
C
C
Islam
M Q
MQ
Szpirer
J
J
Szpirer
C
C
Lundwall
A
A
Levan
G
G
D000818:Animals; D015139:Blotting, Southern; D002874:Chromosome Mapping; D002878:Chromosomes, Human, Pair 1; D003001:Cloning, Molecular; D015342:DNA Probes; D005165:Factor V / Q000235:genetics*; D006801:Humans; D006822:Hybrid Cells; D009693:Nucleic Acid Hybridization; D051381:Rats
D015342:DNA Probes; D005165:Factor V