title: Electrochemical detection for high-performance liquid chromatography of ketoconazole in plasma and saliva.
abstract: Ketoconazole, cis-1-acetyl-4-[4[[2-(2,4-dichlorophenyl)-2-(1H-imidazol- 1-ylmethyl)-1,3-dioxolan-4-yl]methoxy]phenyl]piperazine, a clinically used antifungal agent, is also an inhibitor of steroid hormone biosynthesis. A high-performance liquid chromatographic method is described which resolves ketoconazole with selectivity and high sensitivity provided by the use of electrochemical detection. Ketoconazole can be detected in high-performance liquid chromatography by electrochemical oxidation at a glassy carbon electrode at a potential of +1.0 V. Electrochemical detection offers improved sensitivity and selectivity over ultraviolet absorbance or fluorescence detection after derivatization. The method utilizes a volatile buffer system compatible with postcolumn analyses and an internal standard which is electrochemically active. This technique provides a simple method to assay ketoconazole. Ketoconazole can be detected in human plasma and saliva after a single oral therapeutic dose.
authors.affiliation: Department of Psychiatry, Dartmouth Medical School, Hanover, New Hampshire 03756.
authors.forename: D W
authors.initials: DW
authors.lastname: Hoffman
authors.forename: K L
authors.initials: KL
authors.lastname: Jones-King
authors.forename: C L
authors.initials: CL
authors.lastname: Ravaris
authors.forename: R D
authors.initials: RD
authors.lastname: Edkins
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D007654:Ketoconazole / Q000032:analysis* / Q000097:blood; D012463:Saliva / Q000032:analysis*
chemical_list: D007654:Ketoconazole