Culture of newt cells from different tissues and their expression of a regeneration-associated antigen.
We have established culture conditions for cells from normal limb, early limb regenerate (blastema), heart, and liver of the newt Notophthalmus viridescens. Whereas heart and liver cells had a relatively short life in culture, limb cells have shown no sign of senescence over more than 1 year in culture. Cultured cells from all these tissues express to differing extents the regeneration-associated antigen 22/18. The antigen is intracellular and filamentous, and its expression appears to be regulated by culture density. Furthermore, 22/18 antigen is turned off in limb and blastemal cultures following differentiation into muscle, as also occurs in vivo.
Ferretti
P
P
MRC Cell Biophysics Unit, London, England.
Brockes
J P
JP
D000818:Animals; D000941:Antigens / Q000032:analysis*; D002455:Cell Division; D002470:Cell Survival; D002478:Cells, Cultured; D005121:Extremities / Q000166:cytology* / Q000502:physiology; D007150:Immunohistochemistry; D008099:Liver / Q000166:cytology; D009206:Myocardium / Q000166:cytology; D009673:Notophthalmus viridescens; D012038:Regeneration
D000941:Antigens