[A new immunoluminometric solid phase sandwich assay for antibodies to hepatitis B surface antigen].
A solid phase quantitative immunoluminometric assay for antibodies to hepatitis B surface antigen (Anti-HBsAg) is described. This solid phase assay uses polystyrene balls of 6.4 mm diameter, coated with pure hepatitis B surface antigen (HBsAg) obtained from the plasma of chronic HBsAg carriers. After incubation with patient serum (90 min), HBsAg labelled with 7-(4-aminobutyl-N-ethyl)naphthalene 1,2-dicarboxylic acid hydrazide hemisuccinamide (ABEN-H) was used as the signal carrier. Dilutions of samples and standards were made with sera previously shown to be negative for anti HBsAg. The lower detection limit was about 1.3 U/l the upper limit for undiluted serum about 1000 U/l. The mean coefficient of variation was 5%. The dynamic range (expressed as the signal ratio of the highest standard: zero standard) was 182:1 and covered the range 23-4200 relative light units. The assay was tested for specificity and gave no false-positive reactions in the presence of other hepatitis B antigens and antibodies.
Missler
U
U
Klinische Laboratorien, Medizinische Universität zu Lübeck.
Wood
W G
WG
D000914:Antibodies, Viral / Q000032:analysis*; D005189:False Positive Reactions; D005456:Fluorescent Dyes; D006514:Hepatitis B Surface Antigens / Q000276:immunology*; D006801:Humans; D006834:Hydrazines; D007118:Immunoassay / Q000379:methods; D008163:Luminescent Measurements; D009281:Naphthalenes
D000914:Antibodies, Viral; D005456:Fluorescent Dyes; D006514:Hepatitis B Surface Antigens; D006834:Hydrazines; D009281:Naphthalenes; C054204:7-((N-4-aminobutyl)-N-ethyl)aminonaphthalene-1,2-dicarboxylic acid hydrazide
Ein neuer immunoluminometrischer Festphasen-Sandwich-Assay zum quantitativen Nachweis von Antikörpern gegen das Hepatitis B-Oberflächen (HBs)-antigen.