title: Stimulatory effect of vasoactive intestinal polypeptide on catecholamine secretion from isolated guinea pig adrenal chromaffin cells.
abstract: The stimulatory effect of vasoactive intestinal polypeptide (VIP) on catecholamine (CA) secretion from isolated guinea pig adrenal chromaffin cell was studied. VIP (1-10 microM) induced dose-dependent CA secretion, which was slow and continued for at least 30 min. This VIP-induced CA secretion was dependent on the presence of Ca2+ in the medium, but no significant increase in Ca2+ uptake by the cells was observed during their stimulation with VIP. Studies on the intracellular free Ca2+ level ([Ca2+]i) using fura-2 showed that acetylcholine and muscarine induced a marked increase in the [Ca2+]i, but that VIP induced only a slight increase. Thus VIP may induce CA secretion by increasing the sensitivity of the secretion of CA to Ca2+.
authors.affiliation: Department of Pharmacology, Tokushima University School of Medicine, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Misbahuddin
authors.forename: M
authors.initials: M
authors.lastname: Oka
authors.forename: A
authors.initials: A
authors.lastname: Nakanishi
authors.forename: K
authors.initials: K
authors.lastname: Morita
mesh_terms: D000313:Adrenal Medulla / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000493:pharmacokinetics; D002395:Catecholamines / Q000378:metabolism*; D002469:Cell Separation; D005260:Female; D006168:Guinea Pigs; D066298:In Vitro Techniques; D008297:Male; D009116:Muscarine / Q000494:pharmacology; D009538:Nicotine / Q000494:pharmacology; D014660:Vasoactive Intestinal Peptide / Q000494:pharmacology*
chemical_list: D002395:Catecholamines; D014660:Vasoactive Intestinal Peptide; D009538:Nicotine; D009116:Muscarine; D002118:Calcium