title: Anti-fibrin monoclonal antibodies for radioimmunodetection: preliminary assessment in a rat model system.
abstract: The D Dimer (DD) site formed by linkage of D domains from adjacent fibrin (FN) molecules is unique to cross-linked FN and its degradation products and is not found in FN monomer or fibrinogen (FB). Thus monoclonal antibodies (MAb) reactive to DD should have a very suitable specificity for in vivo thrombus detection. Two anti-DD MAbs have been labelled with 131-I and assessed as scintigraphic agents in a normal rat model system. Each rat received 3 sc implants of antigen covalently coupled to Sepharose beads: 1) Human DD 2) Human FB 3) Glycine (GL) (control). Scintigraphic images taken 7 days after injection of 131-I anti DD MAb showed clear localisation of both anti-DD MAbs to the DD implant rather than to the FB or GL implants with no localisation in normal tissues. This was confirmed in biodistribution studies. Injection of anti-DD MAbs DD-3B6/22 and DD-IC3/108 resulted in DD: blood ratios of 10.4 +/- 0.6 and 4.9 +/- 0.3 respectively. These results suggest that anti-DD MAbs will have potential for thrombus radioimmunodetection.
authors.affiliation: Clinical Immunology Research Centre, Sydney University, N.S.W., Australia.
authors.forename: K Z
authors.initials: KZ
authors.lastname: Walker
authors.forename: F
authors.initials: F
authors.lastname: Khafagi
authors.forename: G J
authors.initials: GJ
authors.lastname: Bautovich
authors.forename: G R
authors.initials: GR
authors.lastname: Boniface
authors.forename: P G
authors.initials: PG
authors.lastname: Bundesen
authors.forename: D B
authors.initials: DB
authors.lastname: Rylatt
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D001666:Binding Sites, Antibody; D004195:Disease Models, Animal; D004343:Drug Implants / Q000008:administration & dosage; D005260:Female; D005337:Fibrin / Q000276:immunology*; D005340:Fibrinogen / Q000008:administration & dosage; D006801:Humans; D007457:Iodine Radioisotopes; D008297:Male; D011877:Radionuclide Imaging; D051381:Rats; D011916:Rats, Inbred F344; D013927:Thrombosis / Q000000981:diagnostic imaging* / Q000378:metabolism; D014018:Tissue Distribution
chemical_list: D000911:Antibodies, Monoclonal; D004343:Drug Implants; D007457:Iodine Radioisotopes; D005337:Fibrin; D005340:Fibrinogen