title: Androgen dependence of specific kallikrein gene family members expressed in rat prostate.
abstract: We have used oligonucleotide probes specific for members of the rat kallikrein/tonin gene family (PS, S1, S2, S3, K1, and P1) to establish which arginyl esteropeptidase (kallikrein-like) genes are expressed in the prostate. We have also compared the expression and androgen dependence of these genes in prostate, submaxillary gland (SMG) and kidney. Only S3 (tonin-like) and P1 (kallikrein-like) are expressed in the prostate, with S3 very much more abundant. Prostatic S3 mRNA disappears after 8 days castration and is restored to intact levels by dihydrotestosterone (DHT) but not estradiol benzoate (EB) for 8 days. Prostate P1 mRNA levels were similarly but not identically affected. All six genes are expressed in the SMG, with PS (true kallikrein) the most abundant. Levels of PS mRNA in SMG are unaffected by castration, DHT, or EB treatment, although mRNA levels of other kallikrein-like (S1, K1, and P1), tonin (S2), and tonin-like (S3) genes fall 40-60% after castration, and are unaffected or partially restored by DHT and/or EB administration. Only PS and K1 are expressed in the kidney, at much lower levels than in the SMG and unaffected by castration or steroids. These studies thus confirm and extend the concept of tissue specificity of arginyl esteropeptidase gene expression, and further demonstrate that the same gene(s) is differentially regulated by androgens in the rat prostate, SMG, and kidney.
authors.affiliation: Medical Research Center, Prince Henry's Hospital, Melbourne, Australia.
authors.forename: J A
authors.initials: JA
authors.lastname: Clements
authors.forename: B A
authors.initials: BA
authors.lastname: Matheson
authors.forename: D R
authors.initials: DR
authors.lastname: Wines
authors.forename: J M
authors.initials: JM
authors.lastname: Brady
authors.forename: R J
authors.initials: RJ
authors.lastname: MacDonald
authors.forename: J W
authors.initials: JW
authors.lastname: Funder
mesh_terms: D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000235:genetics; D013196:Dihydrotestosterone / Q000494:pharmacology; D004958:Estradiol / Q000494:pharmacology; D005796:Genes / Q000187:drug effects; D007610:Kallikreins / Q000235:genetics*; D008297:Male; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D009919:Orchiectomy; D009928:Organ Specificity; D011467:Prostate / Q000187:drug effects / Q000201:enzymology*; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics; D051381:Rats; D012016:Reference Values
chemical_list: D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D013196:Dihydrotestosterone; D004958:Estradiol; D004247:DNA; D007610:Kallikreins