In vitro and in vivo responses of a panel of murine colon tumours to TCNU: a positive correlation.
TCNU is highly active against a panel of three histologically distinct transplantable murine adenocarcinomas of the colon (MAC tumours). Significant reductions in colony formation (greater than 70%) were observed in vitro in all three cell lines following a 1 h exposure to TCNU at experimentally achievable drug C X t values. A good correlation exists between in vivo tumour responses and in vitro cell responses in all cases. Dose-response curves generated at increasing exposure times suggest that no active or long lived products of TCNU are formed as a result of the drug's spontaneous breakdown in vitro (rate of breakdown was 0.078 micrograms min-1 at 37 degrees C). Preliminary studies with the HT-29 human colon cell line have demonstrated that multi-cellular spheroids are more responsive to TCNU (2 X) than the same cells cultured as monolayers.
Phillips
R M
RM
Clinical Oncology Unit, University of Bradford, West Yorkshire, U.K.
Bibby
M C
MC
Double
J A
JA
D000818:Animals; D000970:Antineoplastic Agents / Q000378:metabolism / Q000494:pharmacology*; D002455:Cell Division; D002460:Cell Line; D003110:Colonic Neoplasms / Q000473:pathology*; D003114:Colony-Forming Units Assay; D004305:Dose-Response Relationship, Drug; D051379:Mice; D009368:Neoplasm Transplantation; D009607:Nitrosourea Compounds / Q000378:metabolism / Q000494:pharmacology*; D013654:Taurine / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000187:drug effects*
D000970:Antineoplastic Agents; D009607:Nitrosourea Compounds; D013654:Taurine; C052991:1-(2-chloroethyl)-3-(2-(dimethylaminosulfonyl)ethyl)-1-nitrosourea