title: In vivo action of the anticoccidial diclazuril (Clinacox) on the developmental stages of Eimeria tenella: a histological study.
abstract: Diclazuril, a new benzeneacetonitrile anticoccidial, has potent activity against various stages of Eimeria tenella. A single treatment of experimentally infected chickens during the prepatent phase (up to day 5) results in a complete interruption of the life cycle and oocyst shedding. The first- and second-generation schizonts show extensive degenerative changes that finally result in a complete loss of the parasitic stage. The degeneration is characterized by loss of internal structure, the appearance of many intracytoplasmic vacuoles, and incomplete merogony. The merozoites themselves show similar degenerative changes, including the presence of numerous small vacuoles in the cytoplasm. Diclazuril is also effective against both the micro- and macrogametocytes that have a ballooned appearance and loose their internal structure completely. In the macrogametocytes, wall-forming bodies either do not develop or disappear rapidly. Development of typical caecal lesions is prevented when treatment with diclazuril is initiated before large numbers of second-generation schizonts appear, i.e., day 3. It is concluded that diclazuril is lethal against both the asexual and the sexual stages of E. tenella. At the proposed use level of 1 ppm in the feed, the life cycle is interrupted at a very early stage and lesion development and oocyst shedding are completely prevented.
authors.affiliation: Janssen Research Foundation, Beerse, Belgium.
authors.forename: L
authors.initials: L
authors.lastname: Maes
authors.forename: W
authors.initials: W
authors.lastname: Coussement
authors.forename: O
authors.initials: O
authors.lastname: Vanparijs
authors.forename: R
authors.initials: R
authors.lastname: Marsboom
mesh_terms: D000818:Animals; D002432:Cecum / Q000469:parasitology / Q000473:pathology; D002645:Chickens / Q000469:parasitology*; D003048:Coccidiosis / Q000188:drug therapy / Q000469:parasitology / Q000473:pathology / Q000662:veterinary*; D003049:Coccidiostats / Q000494:pharmacology* / Q000627:therapeutic use; D004539:Eimeria / Q000187:drug effects* / Q000254:growth & development; D007422:Intestines / Q000469:parasitology / Q000473:pathology; D008297:Male; D009570:Nitriles / Q000494:pharmacology* / Q000627:therapeutic use; D011201:Poultry Diseases / Q000188:drug therapy / Q000469:parasitology / Q000473:pathology; D014227:Triazines / Q000494:pharmacology* / Q000627:therapeutic use
chemical_list: D003049:Coccidiostats; D009570:Nitriles; D014227:Triazines; C057884:diclazuril