title: Crystallization and preliminary X-ray analysis of type B influenza virus neuraminidase complexed with antibody Fab fragments.
abstract: Fab fragments from four different monoclonal antibodies have been complexed with influenza B virus neuraminidase (B/Lee/40) and the complexes have been crystallized. Three of the complex crystals are, so far, not suitable for X-ray diffraction studies, but the fourth (B/Lee/40 NA-B1Fab) forms large crystals which diffract X-rays to 3.0 A resolution. The crystals have a space group of F432, a = 441.21 A. Vm calculations show that the asymmetric unit contains two monomeric complexes.
authors.affiliation: John Curtin School of Medical Research, Australian National University, Canberra City.
authors.forename: W G
authors.initials: WG
authors.lastname: Laver
authors.forename: M
authors.initials: M
authors.lastname: Luo
authors.forename: P J
authors.initials: PJ
authors.lastname: Bossart
authors.forename: Y S
authors.initials: YS
authors.lastname: Babu
authors.forename: C
authors.initials: C
authors.lastname: Smith
authors.forename: M A
authors.initials: MA
authors.lastname: Accavitti
authors.forename: P A
authors.initials: PA
authors.lastname: Tulloch
authors.forename: G M
authors.initials: GM
authors.lastname: Air
mesh_terms: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000936:Antigen-Antibody Complex; D000956:Antigens, Viral; D003460:Crystallization; D007140:Immunoglobulin Fab Fragments; D009981:Influenza B virus / Q000201:enzymology*; D008854:Microscopy, Electron; D009439:Neuraminidase / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000936:Antigen-Antibody Complex; D000956:Antigens, Viral; D007140:Immunoglobulin Fab Fragments; D009439:Neuraminidase