Alternative promoter usage by aldolase A during in vitro myogenesis.
Aldolase A in the mouse, as in human and rat, shows tissue-specific variability of message size. In addition, in muscle tissue the mRNA size is also developmentally regulated. In order to determine whether this muscle-specific regulatory mechanism can be reproduced in vitro, we have examined the mRNA species of aldolase A isolated from mouse C2C12 myoblasts and myotubes on Northern blots and by primer extension. We show that aldolase A mRNA increases during in vitro myogenesis; that this induction is accompanied by a change in the message population; and that this change is due to activation of a muscle-specific alternative promoter.
Colbert
M C
MC
Department of Biochemistry, University of Rochester, School of Medicine and Dentistry, New York 14642.
Ciejek-Baez
E
E
D000199:Actins / Q000235:genetics; D000818:Animals; D015152:Blotting, Northern; D002460:Cell Line; D005634:Fructose-Bisphosphate Aldolase / Q000235:genetics*; D005786:Gene Expression Regulation; D066298:In Vitro Techniques; D051379:Mice; D009132:Muscles / Q000201:enzymology* / Q000502:physiology; D011401:Promoter Regions, Genetic
D000199:Actins; D005634:Fructose-Bisphosphate Aldolase