Phenytoin-induced stress protein synthesis in mouse embryonic tissue.
Several proteins have been shown to be synthesized in response to various environmental stimuli, including treatment with teratogens. The role of these proteins in the teratogenic process is unknown. Pregnant A/J mice were treated with either a teratogenic or a non-teratogenic dose of the anticonvulsant drug, phenytoin (PHT). Protein synthesis in embryonic craniofacial (target) tissue or forelimb buds (non-target) was determined by incorporation of radiolabeled leucine and analysis by two-dimensional polyacrylamide gel electrophoresis. Synthesis of three proteins in target tissue and one protein in non-target tissue was stimulated by drug treatment. These results suggest that synthesis of specific stress proteins may serve as biomarkers of drug-target tissue interaction.
Hansen
D K
DK
Division of Reproductive and Developmental Toxicology, National Center for Toxicological Research, Jefferson, Arkansas 72079.
Anson
J F
JF
Hinson
W G
WG
Pipkin
J L
JL
D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D004622:Embryo, Mammalian / Q000187:drug effects / Q000378:metabolism*; D005147:Facial Bones / Q000196:embryology; D005260:Female; D005552:Forelimb / Q000196:embryology; D006360:Heat-Shock Proteins / Q000096:biosynthesis* / Q000302:isolation & purification; D051379:Mice; D008805:Mice, Inbred A; D009687:Nuclear Proteins / Q000096:biosynthesis / Q000302:isolation & purification; D010672:Phenytoin / Q000633:toxicity; D011247:Pregnancy; D012886:Skull / Q000196:embryology; D013723:Teratogens / Q000633:toxicity
D006360:Heat-Shock Proteins; D009687:Nuclear Proteins; D013723:Teratogens; D010672:Phenytoin