A method for the preparation of metaphase chromosomes from rat small intestine.
A major obstacle to successful cytogenetic analysis of small intestinal crypt cells is the acquisition of a sufficient number of high-quality metaphases. A squash procedure has been developed for analysis of metaphase chromosomes from rat small intestine that largely circumvents this difficulty. The method involves a schedule of hypotonic treatment, fixation in ethanol: acetic acid, followed by maceration of the intestinal tissue in 3.5 N HCl. The procedure resulted in large numbers of well-spread, cytoplasm-free metaphases.
Ellsworth
J L
JL
Department of Biological Sciences, Stanford University, California 94305.
Schimke
R T
RT
D000818:Animals; D007413:Intestinal Mucosa / Q000166:cytology / Q000648:ultrastructure; D007583:Jejunum / Q000166:cytology* / Q000648:ultrastructure; D007621:Karyotyping / Q000379:methods*; D008297:Male; D008677:Metaphase; D051381:Rats; D011916:Rats, Inbred F344