Characterization of a heparin-binding growth factor from adenocarcinoma of the kidney.
A polypeptide isolated from tissue extracts of renal adenocarcinoma was mitogenic for BALB/c 3T3 cells and human umbilical vein (HUV) cells in culture. It also demonstrated angiogenic ability using the chorioallantoic membrane bioassay. Using heparin-sepharose affinity chromatography the purified protein eluted with a NaCl concentration between 1.4 and 1.8 M and demonstrated a molecular weight of approximately 17,000 daltons based on SDS polyacrylamide gel electrophoresis. Half maximal stimulation of tritiated thymidine incorporation into BALB/c 3T3 cells was achieved by 1.6 ng./ml. of the heparin binding material. Western blot analysis using antibodies specific to basic fibroblast growth factor (bFGF) only or acidic FGF (aFGF) only demonstrated that the purified protein binds to the former and not the latter. The characteristics of this material, in effect the elution profile off heparin-Sepharose, the molecular weight, angiogenic activity and the results of western blot analysis, suggest that this growth factor is similar to the family of basic fibroblast growth factors.
Mydlo
J H
JH
Urologic Oncology Research Laboratory, Memorial Sloan Kettering Cancer Center, New York, New York 10021.
Heston
W D
WD
Fair
W R
WR
D043925:Angiogenesis Inducing Agents / Q000302:isolation & purification; D000818:Animals; D015153:Blotting, Western; D002292:Carcinoma, Renal Cell / Q000032:analysis*; D002478:Cells, Cultured; D002642:Chick Embryo; D004591:Electrophoresis, Polyacrylamide Gel; D006133:Growth Substances / Q000302:isolation & purification*; D006493:Heparin / Q000302:isolation & purification*; D006801:Humans; D007680:Kidney Neoplasms / Q000032:analysis*; D051379:Mice; D008934:Mitogens / Q000302:isolation & purification
D043925:Angiogenesis Inducing Agents; D006133:Growth Substances; D008934:Mitogens; D006493:Heparin