Small RNAs of the silkworm, Bombyx mori as revealed by in vitro capping and in vitro transcription.
1. We characterized several small RNAs in various tissues and strains of the silkworm, Bombyx mori by the in vitro capping and in vitro transcription. 2. Three subspecies of 7SL RNA, which is believed to be involved in protein secretion, were detected in the commercial strains. Structural differences among the three molecules were due to the nucleotide substitution at two positions or more. 3. A very AU-rich RNA, which is about 500 bases long, was always present in any tissue of Bombyx mori while 55 base RNA was only observed in a substantial amount in the fat body and culture cells. 4. These small RNAs were also observed among the in vitro transcripts of total Bombyx DNA in the cell-free extracts from the silkgland and fat body.
Fujiwara
H
H
Department of Technology, National Institute of Health, Tokyo, Japan.
Maekawa
H
H
Takada
N
N
Miyajima
N
N
Ishikawa
H
H
D000818:Animals; D001483:Base Sequence; D012831:Bombyx / Q000032:analysis* / Q000235:genetics; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D012315:RNA Caps; D012342:RNA, Small Nuclear / Q000235:genetics / Q000302:isolation & purification*; D013045:Species Specificity; D014018:Tissue Distribution; D014158:Transcription, Genetic
D012315:RNA Caps; D012342:RNA, Small Nuclear