[Study of total activity of natural lipid antioxidants by chemiluminescence method].
The total antiradical activity of lipid antioxidants extracted from organs and tissues of fish Coregonus peled (Gmelin) was investigated using chemiluminescence method. It has been established that lipids contain antioxidants of two types. The bioantioxidants of the first type have a comparatively high efficiency constant K7eff. = (2.4-3.2) 10(6) M-1.s-1, whose value is 100 times more than that of the constant of the second type inhibitors K7eff. = (3.5-5.0) 10(4) M-1.s-1. Using the method of thin-layer chromatography such individual antioxidants of lipids as tocopherol, ubiquinon, ubichromenol were separated and quantitatively studied, as well as recorded in the presence of vitamin K, A, cholesterol. It is shown that the quantitative content of high-activity antioxidants in lipid of different kinds substantially varies (0.5-17.1) 10(-4) M; the low level of their content has been recorded for internal fat and brain lipids, the high one--for the lipids present in immature eggs, red muscles and liver.
Burlakova
E B
EB
Storozhok
N M
NM
Khrapova
N G
NG
D000818:Animals; D000975:Antioxidants / Q000032:analysis*; D005399:Fishes; D005609:Free Radicals; D007700:Kinetics; D008055:Lipids / Q000032:analysis*; D049449:Luminescence
D000975:Antioxidants; D005609:Free Radicals; D008055:Lipids
Izuchenie summarnoĭ aktivnosti prirodnykh antioksidantov lipidov khemiliuminestsentnym metodom.