[Phenylalanine hydroxylase of human embryos: its homology with the enzyme from the liver of adults].
Phenylalanine hydroxylase (PH) activity was discovered in the liver of 7-12 week old human embryos. Embryonic and adult PHs were identical, as shown by immunoelectrophoresis. Unlike the adult liver PH, the PH content of the extract of cytoplasmic proteins of embryonic liver was reduced but the specific activity was increased more than by one order of magnitude. H (57,000 D) and L (55,000 D) subunits were detected by immunoblotting. The L subunit predominates in the extract of membrane proteins of embryonic liver. Hence, the major part of phenylalanine oxidizing activity in the embryonic liver is related to the enzyme immunochemically identical with the PH of adult liver but differing from it in some structural and functional properties.
Laptev
A V
AV
Chestkov
V V
VV
Shchepkina
Iu V
IuV
Shishkin
S S
SS
D000328:Adult; D003593:Cytoplasm / Q000201:enzymology; D004622:Embryo, Mammalian / Q000201:enzymology*; D005865:Gestational Age; D006801:Humans; D015151:Immunoblotting; D007122:Immunoelectrophoresis; D008099:Liver / Q000201:enzymology*; D010651:Phenylalanine Hydroxylase / Q000032:analysis* / Q000302:isolation & purification
D010651:Phenylalanine Hydroxylase
Fenilalaningidroksilaza émbrionov cheloveka: gomologiia s fermentom iz pecheni vzroslykh liudeĭ.