Bass liver 6-phosphogluconate dehydrogenase: inhibition by nucleoside phosphates and by fructose 1,6 bisphosphate.
Nucleoside 5'-triphosphates, 5'-diphosphates and 5'-monophosphates are inhibitors of the 6-phosphogluconate dehydrogenase enzyme from bass liver. The 2'- and 3'-monophosphates of adenosine and guanosine are also inhibitory, the 2'-isomers being especially potent inhibitors. The catalytic activity of 6-phosphogluconate dehydrogenase has been found to be markedly inhibited by fructose 1, 6 bisphosphate. As the Km for 6-phosphogluconate, the Ki for fructose 1,6 bisphosphate and the concentration of both compounds in bass liver are all comparable, it appears that the inhibition of 6-phosphogluconate dehydrogenase by fructose 1,6 bisphosphate may be of significance in the regulation of carbohydrate metabolism in bass liver.
Medina Puerta
M M
MM
Departamento de Bioquímica, Facultad de Veterinaria, Universidad Complutense de Madrid, Spain.
Gallego-Iniesta
M
M
Garrido-Pertierra
A
A
D000818:Animals; D001492:Bass / Q000378:metabolism*; D001665:Binding Sites; D005635:Fructosediphosphates / Q000494:pharmacology; D066298:In Vitro Techniques; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D009711:Nucleotides / Q000494:pharmacology; D010473:Perciformes / Q000378:metabolism*; D010734:Phosphogluconate Dehydrogenase / Q000037:antagonists & inhibitors*
D005635:Fructosediphosphates; D009711:Nucleotides; D010734:Phosphogluconate Dehydrogenase; C029063:fructose-1,6-diphosphate