[Chemico-enzymatic synthesis and cloning of human angiogenin gene in M13mp8 phage].
The nucleotide sequence coding for human angiogenin has been deduced from the published amino acid sequence with the use of codons preferentially utilized in highly expressed E. coli genes. It was divided into forty-three oligonucleotides, which were synthesized by automatic gene assembler and then joined by DNA ligase into three double-stranded blocks, the blocks were consequently cloned and ligated in M13mp8 phage, and the resultant 389-bp DNA sequence coding for human angiogenin was analysed by chain-terminator sequencing technique.
Kovalenko
S P
SP
Gorn
V V
VV
Karginov
V A
VA
Morozov
I V
IV
Zarytova
V F
VF
D000595:Amino Acid Sequence; D043925:Angiogenesis Inducing Agents / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D003062:Codon; D003090:Coliphages / Q000235:genetics; D005796:Genes; D005813:Genes, Synthetic; D005822:Genetic Vectors; D006133:Growth Substances / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000235:genetics*; D012259:Ribonuclease, Pancreatic
D043925:Angiogenesis Inducing Agents; D003062:Codon; D006133:Growth Substances; D009363:Neoplasm Proteins; C046366:angiogenin; D012259:Ribonuclease, Pancreatic
Khimiko-fermentativnyĭ sintez i klonirovanie gena angiogenina cheloveka v sostave faga M13mp8.