title: Femtomole analysis of diclofenac in human plasma by gas chromatography/negative ion chemical ionization/mass spectrometry using (18O2)diclofenac as internal standard.
abstract: A stable isotope dilution gas chromatography/negative ion chemical ionization/mass spectrometry assay for diclofenac in human plasma is described. The preparation of (18O2)diclofenac and its use as an internal standard for quantitative gas chromatography/mass spectrometry is shown. A sample processing and derivatization sequence with product recovery of 84.7% was found. The method presented permits quantitative measurement of diclofenac in human plasma at the lower femtomole level. Plasma levels of diclofenac after administration of diclofenac gel were estimated.
authors.affiliation: Univ. Kinderklinik, Abt. Massenspektrometrie, Graz, Austria.
authors.forename: H J
authors.initials: HJ
authors.lastname: Leis
authors.forename: H
authors.initials: H
authors.lastname: Gleispach
authors.forename: E
authors.initials: E
authors.lastname: Malle
authors.forename: V
authors.initials: V
authors.lastname: Nitsche
mesh_terms: D004008:Diclofenac / Q000097:blood*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D066298:In Vitro Techniques; D007553:Isotope Labeling; D010103:Oxygen Isotopes
chemical_list: D010103:Oxygen Isotopes; D004008:Diclofenac