Measurement of septal release of vasopressin and oxytocin by the push-pull technique following electrical stimulation of the paraventricular nucleus of rats.
The release of vasopressin (AVP) and oxytocin (OXT) within the septum was studied with the push-pull perfusion technique in 6 conscious, freely behaving male rats. Push-pull perfusion was performed via a chronically implanted cannula and samples collected for 3 consecutive 30-min periods. Stimulating electrodes were implanted in both the left and right paraventricular nuclei 4 days before the experiment. Bilateral electrical stimulation (10-s trains every 4 min) of the paraventricular nuclei during the second 30-min period resulted in a significant increase in the release of both AVP and OXT (128% and 159% of control values respectively); release returned to the pre-stimulation value during the final 30-min collection.
Neumann
I
I
Department of Cell Biology and Regulation, Karl Marx University, Leipzig, G.D.R.
Schwarzberg
H
H
Landgraf
R
R
D000818:Animals; D001127:Arginine Vasopressin / Q000378:metabolism*; D004558:Electric Stimulation; D008297:Male; D010121:Oxytocin / Q000378:metabolism*; D010286:Paraventricular Hypothalamic Nucleus / Q000378:metabolism* / Q000502:physiology; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains
D001127:Arginine Vasopressin; D010121:Oxytocin