In-vivo and in-vitro association between leukemic cells and marrow stromal cells: a murine model.
Several reports indicate that marrow stromal cells are conducive to development of both myeloid and lymphoid leukemic cell lines in vitro. We present data on an in-vitro/in-vivo murine model that establishes that: (i) murine LL cell lines (L5178Y, P388 and L1210) invade the bone marrow when injected via the tail vein; (ii) degree of marrow invasion by LL can be determined quantitatively; (iii) there is a close association or adherence between MSC and LL cells in vivo; and (iv) in-vitro adherence of murine LL cells to MSC can be quantitated. This in-vitro/in-vivo murine model will be helpful in deciphering the importance of heterotypic adherence between marrow stromal cells and leukemic cells in vivo.
Juneja
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Mary Jeane Price-Jones Kempner Laboratory for Hematology Research, University of Texas Medical Branch, Galveston.
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D000818:Animals; D001853:Bone Marrow / Q000473:pathology* / Q000648:ultrastructure; D002448:Cell Adhesion; D002450:Cell Communication; D002460:Cell Line; D005109:Extracellular Matrix / Q000473:pathology* / Q000648:ultrastructure; D007939:Leukemia L1210 / Q000473:pathology; D007940:Leukemia L5178 / Q000473:pathology; D007941:Leukemia P388 / Q000473:pathology; D007942:Leukemia, Experimental / Q000473:pathology*; D007951:Leukemia, Myeloid / Q000473:pathology; D008113:Liver Neoplasms / Q000556:secondary; D051379:Mice; D008811:Mice, Inbred DBA; D009361:Neoplasm Invasiveness; D009368:Neoplasm Transplantation; D013160:Splenic Neoplasms / Q000556:secondary; D014407:Tumor Cells, Cultured / Q000473:pathology* / Q000637:transplantation / Q000648:ultrastructure