Radioimmunoassay for melatonin and its application to fowl pineal research.
A radioimmunoassay for melatonin has been developed after raising anti-melatonin antibodies in rabbit. Melatonin was extracted from serum or pineal gland of chickens (Gallus domesticus). The radioimmunoassay was performed by using 3H-melatonin as tracer. The standard curve covered the range 0.022-0.345 pmol/vial and the KD value for melatonin was estimated at 1.37 x 10(10) l/mol. The antiserum specificity has been analysed, none of the common melatonin analogues influencing this method of melatonin measurement. The intra-assay variability was 7.2% for serum samples and 8.6% for pineal extract. The inter-assay variability for this biological sample was 15.3% and 6.4% respectively.
Sigüenza
A F
AF
Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias (Sección de Químicas), Valladolid, Spain.
Recio
J M
JM
Agapito
M T
MT
D000818:Animals; D002645:Chickens / Q000097:blood*; D005069:Evaluation Studies as Topic; D008550:Melatonin / Q000032:analysis* / Q000097:blood; D010870:Pineal Gland / Q000032:analysis*; D011863:Radioimmunoassay / Q000379:methods*
D008550:Melatonin