Cloning of glucocorticoid-responsive mRNA in the rat thymus.
To elucidate the molecular mechanism of rat thymus involution induced by administration of glucocorticoid, we screened a cDNA library for polysomal poly(A)+RNA from adrenalectomized rat thymi by a differential colony hybridization method. Labeled cDNAs for mRNAs isolated from thymi of adrenalectomized rats and rats receiving dexamethasone (Dex) treatment were used as probes. Eight cDNA clones for mRNAs which had a diminished response to administration of Dex were isolated. The relative concentration of cloned mRNAs in the thymic polysomal RNA was significantly decreased 3 h after hormone administration, while changes in the nuclear RNA were not significant after Dex administration. One of the selected cDNA clones designated pRTGR-8 corresponded to mRNA of about 3,000 nucleotides, and a nuclear run-off transcription assay indicated that the rate of transcription of pRTGR-8 RNA was repressed by Dex administration. The cloned cDNAs obtained in this experiment may provide useful probes for studying the negative regulation mechanism of gene expression by glucocorticoids.
Hoshikawa
Y
Y
Division of Physiology, Tottori University School of Medicine, Yonago, Japan.
Izawa
M
M
Ichii
S
S
D000818:Animals; D015152:Blotting, Northern; D002458:Cell Fractionation; D003001:Cloning, Molecular / Q000187:drug effects*; D004247:DNA / Q000187:drug effects / Q000235:genetics; D003907:Dexamethasone / Q000494:pharmacology; D005938:Glucocorticoids / Q000235:genetics / Q000494:pharmacology*; D008297:Male; D012333:RNA, Messenger / Q000032:analysis / Q000187:drug effects / Q000235:genetics*; D051381:Rats; D011919:Rats, Inbred Strains; D013950:Thymus Gland / Q000166:cytology* / Q000187:drug effects; D014158:Transcription, Genetic / Q000187:drug effects
D005938:Glucocorticoids; D012333:RNA, Messenger; D003907:Dexamethasone; D004247:DNA