[Correlation between the proliferative activity and protein kinase C activities in ethidium bromide-sensitive and -resistant cells].
The protein kinase C (PK C) activity was determined in the cytosolic and membrane fractions of L- and CHO-K1-cells, both sensitive and resistant to ethidium bromide (EB). In the resistant cells (Lebr-25 and Cebr) a decreased enzyme activity was found in addition to alteration of the enzyme elution profile in the membrane preparations purified by DE-52 cellulose column chromatography. Methyltestosterone treated cells had a decreased enzyme activity in nonpurified membrane preparations in Lebr-25 cells, whereas the enzyme quantity in purified preparations remained the same. The decreased PK C activity on membranes correlates with the rapid proliferation of the resistant cells. The differences found between Lebr-25 and Cebr-cell lines in proliferation response to methyltestosterone correspond to the change of PK C developed due to hormone treatment.
Nekrasova
T P
TP
Ganelina
L Sh
LSh
Ignatova
T N
TN
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects / Q000201:enzymology; D002478:Cells, Cultured; D002852:Chromatography, Ion Exchange; D006224:Cricetinae; D003412:Cricetulus; D003600:Cytosol / Q000187:drug effects / Q000201:enzymology; D004351:Drug Resistance; D004996:Ethidium / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D008777:Methyltestosterone / Q000494:pharmacology; D051379:Mice; D011493:Protein Kinase C / Q000032:analysis / Q000378:metabolism*
D011493:Protein Kinase C; D004996:Ethidium; D008777:Methyltestosterone
Korreliatsiia mezhdu proliferativnoĭ aktivnost'iu i aktivnost'iu proteinkinazy C v kletkakh, chuvstvitel'nykh i ustoĭchivykh k bromistomu étidiiu.