[The effect of gamma-irradiation on the poly(ADP-ribosylation) of nuclear proteins in rat thymocytes].
Differences in the spectra of modified nuclear proteins of thymocytes of control and irradiated rats were investigated using antibodies specific for poly(ADP-ribose) and incorporation of a label from 14C-NAD in vitro. Two classes of modified proteins were identified differing in the rate of the polymer metabolism and the degree of poly(ADP-ribosylation). No postirradiation changes were detected in poly(ADP-ribosylation) of the nuclear sap proteins and chromatin. A pronounced increase in modification of proteins with the molecular mass of 72 and 83 kD and a sharp decrease in poly(ADP-ribosylation) of a protein group with the molecular mass of 47 to 65 kD were detected within the nuclear matrix by the second hour following irradiation. A study was made of the localization of modified proteins in polydeoxynucleotide fractions of different sizes (mononucleosomes and their oligomers).
Beletskiĭ
I P
IP
Nelipovich
P A
PA
Umanskiĭ
S R
SR
D000818:Animals; D000906:Antibodies / Q000378:metabolism; D002250:Carbon Radioisotopes; D003037:Cobalt Radioisotopes; D005720:Gamma Rays; D008297:Male; D009243:NAD / Q000378:metabolism; D009687:Nuclear Proteins / Q000378:metabolism / Q000528:radiation effects*; D009702:Nucleoside Diphosphate Sugars / Q000378:metabolism*; D011064:Poly Adenosine Diphosphate Ribose / Q000276:immunology / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013950:Thymus Gland / Q000378:metabolism / Q000528:radiation effects*
D000906:Antibodies; D002250:Carbon Radioisotopes; D003037:Cobalt Radioisotopes; D009687:Nuclear Proteins; D009702:Nucleoside Diphosphate Sugars; D009243:NAD; D011064:Poly Adenosine Diphosphate Ribose
Vliianie gamma-oblucheniia na poli(ADF-ribozilirovanie) iadernykh belkov timotsitov krys.