title: Analysis of the fifth cell cycle of mouse development.
abstract: The 5th cell cycle of mouse development was analyzed to determine the lengths of each cell cycle phase. The DNA content of Feulgen-stained blastomere nuclei was measured at various times throughout the cell cycle by microdensitometry. To achieve precise timing of the start of the 5th cell cycle, experiments utilized isolated 16-cell blastomeres and cell pairs obtained by in-vitro division of isolated 8-cell blastomeres. The following estimates were made for a mixed population of polar and apolar 16-cell blastomeres: G1, less than or equal to 2 h; S, 8-9 h; G2 + M, 2 h. No significant difference was found in the timing of DNA synthesis between polar and apolar cells or between cell pairs and whole embryos.
authors.affiliation: Department of Anatomy, University of Cambridge, U.K.
authors.forename: J C
authors.initials: JC
authors.lastname: Chisholm
mesh_terms: D000818:Animals; D001757:Blastomeres / Q000166:cytology*; D002453:Cell Cycle; D002455:Cell Division; D002467:Cell Nucleus / Q000032:analysis; D004247:DNA / Q000032:analysis; D003720:Densitometry; D051379:Mice; D008815:Mice, Inbred Strains
chemical_list: D004247:DNA