Alterations in the d(CpGpT) structure in solution as a result of [PtCl(diethylenetriamine)]+ binding.
The trinucleotide d(CpGpT) reacts with [PtCl(dien)]Cl (dien = diethylenetriamine) to yield as a single adduct Pt(dien)[d(CpGpT)-N7(2)]. The structure of this adduct in solution has been analysed with the aid of NMR spectroscopy and compared with that of the unmodified trinucleotide. A change in the population of the S conformer of the guanosine deoxyribose ring and a syn preference of the guanine residue are the most important changes occurring upon platination. As a result the dC-dG stack disappears, whereas the dG-dT stack is hardly affected. The CD spectra of both platinated and free d(CpGpT) confirm the different nature of the two molecules.
van Garderen
C J
CJ
Gorlaeus Laboratoria der Rijks-universiteit Leiden, The Netherlands.
Altona
C
C
Reedijk
J
J
D001665:Binding Sites; D002236:Carbohydrate Conformation; D002942:Circular Dichroism; D002945:Cisplatin / Q000031:analogs & derivatives* / Q000032:analysis; D003848:Deoxyguanine Nucleotides / Q000032:analysis; D006027:Glycosides / Q000032:analysis; D009682:Magnetic Resonance Spectroscopy; D009838:Oligodeoxyribonucleotides / Q000032:analysis*; D010984:Platinum / Q000032:analysis; D012996:Solutions; D013329:Structure-Activity Relationship; D013696:Temperature; D013816:Thermodynamics
D003848:Deoxyguanine Nucleotides; D006027:Glycosides; D009838:Oligodeoxyribonucleotides; D012996:Solutions; C032799:chlorodiethylenetriamine platinum; D010984:Platinum; D002945:Cisplatin