Cryopreservation of Leucocytozoon caulleryi sporozoites.
Leucocytozoon caulleryi sporozoites that had been stored at -196 degrees C or -80 degrees C for 6 or 12 months in Eagle's minimum essential medium or Medium 199 supplemented with 5% glycerol and 10% chicken serum showed infectivity to chickens. Glycerol at a concentration of 10% and dimethyl sulfoxide at 10% and 5% were found to be ineffective cryoprotective agents for the low temperature preservation of sporozoites. Sporozoites isolated from the intact females of Culicoides arakawae, which had been stored at -80 degrees C for 6 or 12 months without cryoprotective agents, retained their infectivity. No differences were observed in the prepatent period, duration of parasitemia, and presence of serum-soluble antigens between chickens infected with frozen sporozoites and those infected with fresh sporozoites.
Moril
T
T
Department of Parasitology, Kyorin University School of Medicine, Tokyo, Japan.
Matsui
T
T
Iijima
T
T
Abe
N
N
Yoshizawa
A
A
Chikatsune
M
M
Watanabe
M
M
D000818:Animals; D002519:Ceratopogonidae; D002645:Chickens / Q000469:parasitology*; D056890:Eukaryota / Q000502:physiology*; D005260:Female; D005615:Freezing; D008297:Male; D011201:Poultry Diseases / Q000469:parasitology*; D011309:Preservation, Biological; D011528:Protozoan Infections / Q000469:parasitology; D011529:Protozoan Infections, Animal