[Comparative characterization of the morphologic changes induced by the cytotoxic action of a filtrate of Clostridium difficile strain B in cell cultures].
Investigations carried out by the authors have demonstrated the possibility of the simultaneous evaluation of the results of the quantitative and qualitative characterization of the cytotoxic action of the filtrate of C. difficile strain B in the cultures of diploid human cells and cells Fl. The action of the filtrate used in the same dilution (1:1,000) over equal incubation periods (15 minutes) has resulted in the appearance of different morphological changes in each of these cultures. The degree of the manifestation of the cytotoxic action of the filtrate and the consequences of this action depend not only on the dose of the filtrate and the duration of the contact, but also on the kind of cell cultures used in the experiment. The 15-minute contact of human diploid cell culture with the filtrate leads to irreversible lesions of the cells. The rounding of cells Fl, observed during the first 15 minutes of the action of the filtrate, is not fatal for them; in the overwhelming majority of these cells the capacity for proliferation restores at the expiration of a certain period (about 3 weeks), and their adhesive properties increase.
Chervonskaia
G P
GP
Tret'iakov
V A
VA
Mironova
L L
LL
D002470:Cell Survival; D002478:Cells, Cultured; D003013:Clostridium; D004171:Diploidy; D006801:Humans
Sravnitel'naia kharakteristika morfologicheskikh izmeneniĭ, vyzvannykh titotoksichekim deĭstviem fil'trata Clostridium difficile shtamma B v kul'turakh kletok.