The use of amphotericin B in prevention of Trypanosoma theileri growth in bovine cell culture.
Trypanosoma theileri is an ubiquitous blood parasite of cattle. This parasite grows easily in RPMI medium and perturbs lymphocyte tests based on [3H]thymidine uptake. This paper reports that 0.5 microgram ml-1 amphotericin B in the medium is adequate to get Trypanosoma growth inhibition without alteration of either mitogenic or allogenic stimulation of bovine lymphoid cells in vitro.
Lostrie-Trussart
N
N
Département de Microbiologie, Facultés Universitaires Notre-Dame de la Paix, Namur, Belgium.
Letesson
J J
JJ
Didembourg
C
C
Depelchin
A
A
D000666:Amphotericin B / Q000494:pharmacology*; D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000276:immunology / Q000469:parasitology*; D008934:Mitogens / Q000494:pharmacology; D013936:Thymidine / Q000378:metabolism; D014345:Trypanosoma / Q000187:drug effects / Q000254:growth & development*
D008934:Mitogens; D000666:Amphotericin B; D013936:Thymidine