title: Expression of human G gamma globin genes carrying a T or a C at position -158 in COS and MEL cells.
abstract: Increasing data strongly suggest that, in adults, the G gamma/A gamma globin chains ratio is genetically controlled by determinants linked in cis to the beta-globin gene cluster. The presence of the C----T substitution at position -158 5' to the G gamma globin gene in subjects displaying high G gamma/A gamma globin chains ratio led to the hypothesis that this substitution is involved in the determination of high G gamma globin gene expression. In order to test this hypothesis, we have compared the expression of two G gamma globin genes carrying a C or a T at position -158, upon transfection into a COS cell line or stable integration into a MEL cell line. Our results showed no significant effect of the nucleotide at position -158 on the level of human G gamma mRNA produced in these cells.
authors.affiliation: Laboratoire de Biologie Cellulaire (C.N.R.S.-UMR0004), Université de Lyon-I, Villeurbanne, France.
authors.forename: J
authors.initials: J
authors.lastname: Starck
authors.forename: C S
authors.initials: CS
authors.lastname: Qiao
authors.forename: F
authors.initials: F
authors.lastname: Morlé
authors.forename: P
authors.initials: P
authors.lastname: Charnay
authors.forename: J
authors.initials: J
authors.lastname: Godet
mesh_terms: D002460:Cell Line; D003562:Cytidine; D005914:Globins / Q000235:genetics*; D006801:Humans; D007074:Immunoglobulin G / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism; D013936:Thymidine
chemical_list: D007074:Immunoglobulin G; D012333:RNA, Messenger; D003562:Cytidine; D005914:Globins; D013936:Thymidine