Simple radioimmunobinding assay for quantitation of sperm antibodies of IgG immunoglobulin class.
The immunobead test (IBT) is an excellent test for initial sperm antibody screening and evaluation but is impractical when used to quantitate antibody levels using a twofold dilution series. The aim of this study, therefore, was to develop a relatively simple radioimmunobinding assay that would allow quantitation of sperm antibodies of IgG immunoglobulin class, which predominate in male sera. [125I]-Protein G was chosen as the radioligand because it binds to all IgG subclasses, but not to IgA or IgM. The results of the investigation indicate that the [125I]-protein G assay (PGA) allows efficient quantitation of sperm antibodies, as evidenced by a highly significant (P less than .0001) correlation (Spearman's, Rs = 0.94) between the PGA results and IgG-IBT titres.
Clarke
G N
GN
Department of Pathology, Royal Women's Hospital, Carlton, Australia.
D001323:Autoantibodies / Q000032:analysis* / Q000592:standards; D001426:Bacterial Proteins / Q000592:standards; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis* / Q000145:classification / Q000592:standards; D007552:Isotonic Solutions; D008297:Male; D008863:Microspheres; D011863:Radioimmunoassay / Q000379:methods / Q000592:standards; D011869:Radioligand Assay / Q000379:methods / Q000592:standards; D015203:Reproducibility of Results; D012661:Semen / Q000276:immunology; D012710:Serum Albumin, Bovine; D013094:Spermatozoa / Q000276:immunology*; D013291:Streptococcus
D001323:Autoantibodies; D001426:Bacterial Proteins; C034284:IgG Fc-binding protein, Streptococcus; D007074:Immunoglobulin G; D007552:Isotonic Solutions; C037692:Tyrode's solution; D012710:Serum Albumin, Bovine