title: RNA labelling with 3H-orotic acid and 3H-fluorouracil of rat liver tumour following transient hepatic arterial ischaemia.
abstract: Recent studies have demonstrated that transient (1 h) hepatic arterial ischaemia is followed by increased incorporation of labelled thymidine into tumour DNA 24 h later. The present study aimed at investigating if and when there is a corresponding increase in incorporation of labelled 5-fluorouracil (5-FU) or orotic acid into tumour tissue. Incorporation was studied at 0, 6, 24 and 48 h after discontinuation of transient hepatic arterial ischaemia in Wistar-Furth rats having liver tumour. The transient ischaemia was followed by increased RNA and RNA/DNA ratios in normal liver but not in tumour. Incorporation of orotic acid into RNA and DNA was unchanged in tumour but increased at 0 and 6 h in normal liver. Incorporation of 5-FU decreased with time in liver and tumour DNA and in tumour RNA. It is suggested that 5-FU has an optimal effect on tumour tissue when infused immediately after transient hepatic arterial ischaemia.
authors.affiliation: Department of Surgery, Lund University, Sweden.
authors.forename: P
authors.initials: P
authors.lastname: Mack
authors.forename: K G
authors.initials: KG
authors.lastname: Tranberg
authors.forename: U
authors.initials: U
authors.lastname: Strenram
authors.forename: B
authors.initials: B
authors.lastname: Jeppsson
authors.forename: D
authors.initials: D
authors.lastname: Widlus
authors.forename: S
authors.initials: S
authors.lastname: Bengmark
mesh_terms: D000818:Animals; D004273:DNA, Neoplasm / Q000378:metabolism; D005472:Fluorouracil / Q000493:pharmacokinetics*; D006499:Hepatic Artery; D007511:Ischemia / Q000150:complications*; D008113:Liver Neoplasms / Q000098:blood supply / Q000378:metabolism*; D008297:Male; D009963:Orotic Acid / Q000493:pharmacokinetics*; D012334:RNA, Neoplasm / Q000378:metabolism*; D051381:Rats; D011920:Rats, Inbred WF; D013997:Time Factors
chemical_list: D004273:DNA, Neoplasm; D012334:RNA, Neoplasm; D009963:Orotic Acid; D005472:Fluorouracil