A new macromolecular antitumor antibiotic, C-1027. II. Isolation and physico-chemical properties.
A new macromolecular antibiotic C-1027 was obtained from the broth filtrate of Streptomyces globisporus C-1027 by precipitation with ammonium sulfate, DEAE-cellulose column chromatography and gel filtration chromatography on a Sephadex G-75 column. This antibiotic, prepared as a white powder, is an acidic polypeptide having an isoelectric point of pH 3.5-3.7 and a molecular weight of 15,000 as determined by SDS-polyacrylamide gel electrophoresis and gel filtration chromatography. The acid hydrolysate of the purified antibiotic C-1027 contained no methionine or tryptophan. From the physico-chemical data, it may be considered to possess a very labile non-protein chromophore.
Otani
T
T
Biological Research Laboratory, Taiho Pharmaceutical Co., Ltd., Tokushima, Japan.
Minami
Y
Y
Marunaka
T
T
Zhang
R
R
Xie
M Y
MY
D000596:Amino Acids / Q000032:analysis; D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D000903:Antibiotics, Antineoplastic / Q000032:analysis / Q000302:isolation & purification*; D055598:Chemical Phenomena; D002621:Chemistry; D004355:Drug Stability; D053281:Enediynes; D005285:Fermentation; D008970:Molecular Weight; D011506:Proteins / Q000302:isolation & purification
D000596:Amino Acids; D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D000903:Antibiotics, Antineoplastic; D053281:Enediynes; D011506:Proteins; C058024:C 1027