The use of specimen tilt in transmission electron microscopy of the central nervous system.
Thin sections of nervous tissue were viewed at different tilt angles using a transmission electron microscope equipped with a eucentric goniometer stage. In a comparison study of various degrees of tilt, one can observe additional morphological features within synaptic profiles, define subsynaptic structures such as Taxi-bodies, and clearly see the crystalline formation of cytochemical tracers. This study demonstrates the value of tilting thin-sections in the analysis of synapses and other biological material at the ultrastructural level.
Milroy
A M
AM
Department of Anatomy, University of California, San Francisco 94143.
Ralston
D D
DD
D000818:Animals; D001921:Brain / Q000648:ultrastructure*; D002415:Cats; D008253:Macaca mulatta; D008854:Microscopy, Electron; D013048:Specimen Handling / Q000379:methods*; D013569:Synapses / Q000648:ultrastructure