The interaction of nuclear proteins with essential promoter element of the chicken cardiac myosin light chain 2 gene is involved in muscle-specific transcription.
A quantitative microinjection procedure has been developed to demonstrate muscle-specific transcription of the myosin light chain 2-A (MLC2-A) promoter in differentiated chicken primary breast muscle cells. Nuclear protein binds to the distal region of the required promoter sequence but not to a mutated version of this sequence. The functional significance of this specific DNA-protein interaction for the promoter activity is demonstrated by 'in vivo' competition of microinjected MLC-CAT reporter construct together with excess of synthetic oligonucleotides encompassing the protein binding sites.
Braun
T
T
Department of Toxicology, Medical School, University of Hamburg, FRG.
Buschhausen-Denker
G
G
Tannich
E
E
Arnold
H H
HH
D000818:Animals; D001483:Base Sequence; D002645:Chickens; D005796:Genes; D008969:Molecular Sequence Data; D009132:Muscles / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism*; D015879:Myosin Subfragments; D009218:Myosins / Q000235:genetics*; D009687:Nuclear Proteins / Q000502:physiology*; D010446:Peptide Fragments / Q000235:genetics*; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D014158:Transcription, Genetic
D015879:Myosin Subfragments; D009687:Nuclear Proteins; D010446:Peptide Fragments; D009218:Myosins