Objective criteria for in-vitro responses in human tumor colony-forming assays.
The application of rigorous statistical criteria to the design and analysis of in-vitro assay techniques can lead to more reliable assessments of their utility. The authors demonstrate that an objective criterion for in-vitro drug sensitivity would yield less pronounced false-negative error rates than a fixed-cutoff rule, and would therefore be more suitable for screening of putative chemotherapeutic agents.
Koziol
J A
JA
Department of Basic and Clinical Research, Scripps Clinic and Research Foundation, La Jolla, California 92037.
Petkau
A J
AJ
Taetle
R
R
D003114:Colony-Forming Units Assay; D003662:Decision Theory; D004305:Dose-Response Relationship, Drug; D005188:False Negative Reactions; D006801:Humans; D015233:Models, Statistical; D011237:Predictive Value of Tests; D011336:Probability; D014410:Tumor Stem Cell Assay