A comparison of fluorographic methods for the detection of 35S-labeled proteins in polyacrylamide gels.
Eight different methods of fluorographic enhancement of sensitivity to 35S decay after gel electrophoresis were compared. Using Kodak X-Omat AR X-ray film, we found that some fluors were about equivalent to 2,5-diphenyloxazole/dimethyl sulfoxide embedding, whereas several other fluors were not quite as effective, but still were significantly more sensitive than control autoradiography. The most sensitive procedures can yield a detectable darkening of film with less than 1 dpm/mm2 of 35S after a 1-week exposure.
Perng
G G
GG
Department of Physiology, University of Miami, Florida 33101.
Rulli
R D
RD
Wilson
D L
DL
Perry
G W
GW
D000818:Animals; D001001:Anura; D001345:Autoradiography; D004121:Dimethyl Sulfoxide; D015180:Electrophoresis, Gel, Two-Dimensional; D010080:Oxazoles; D011506:Proteins / Q000032:analysis*; D013050:Spectrometry, Fluorescence; D013462:Sulfur Radioisotopes
D010080:Oxazoles; D011506:Proteins; D013462:Sulfur Radioisotopes; C009012:2,5-diphenyloxazole; D004121:Dimethyl Sulfoxide