The energy requirement for protein synthesis in rat brain mitochondria purified by phase partition.
Brain mitochondria purified by phase partition showed a higher rate of 14C-leucine incorporation into proteins with an endogenous source of ATP than with an exogenous ATP-generating system. Under the former conditions the presence of atractyloside increased the 14C-leucine incorporation into proteins. The effects of different valinomycin concentrations plus attractyloside on intramitochondrial ATP levels and 14C-leucine incorporation into proteins have been studied. The results indicate that the protein synthesis in brain mitochondria is dependent on the intramitochondrial ATP concentration.
Peñas
M
M
Departamento de Bioquímica, Facultad de Farmacia, Universidad Complutense, Madrid, Spain.
Sánchez-Prieto
J
J
Martín-González
E
E
Fernández
M
M
López-Pérez
M J
MJ
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001278:Atractyloside / Q000494:pharmacology; D001921:Brain / Q000378:metabolism / Q000648:ultrastructure*; D004734:Energy Metabolism; D007930:Leucine / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism*; D014176:Protein Biosynthesis; D051381:Rats; D011919:Rats, Inbred Strains; D014634:Valinomycin / Q000494:pharmacology
D001278:Atractyloside; D014634:Valinomycin; D000255:Adenosine Triphosphate; D007930:Leucine