Mucins obtained from patients with enterocutaneous urinary diversions.
The ureteroenterocutaneostomy is a surgical procedure for urinary diversion by which an intestinal segment is used as a conduit or a reservoir for urine. Histological studies on colonic segments have shown a well preserved mucosal surface with numerous goblet cells and urine from these patients contains a mucus-like sediment. The present study demonstrates that such gels are composed of mucins occurring predominantly as a glycoprotein complex which is 'insoluble' in 6 M guanidinium chloride. The complex was brought into solution by reduction of disulphide bonds and the fragments (subunits) obtained behaved as typical mucins when subjected to density-gradient centrifugation in CsCl/4 M guanidinium chloride. High-Mr glycopeptides obtained after trypsin digestion of subunits expressed the ABH and Lewis antigens, in accordance with the patients' blood-group phenotype and with the regional distribution known from previous immunohistochemical studies on normal colonic mucosa. A heterogeneous population of mucin glycopeptides was revealed by using high-performance ion-exchange chromatography and the major carbohydrate-containing peak did not coincide with that expressing the blood-group activity.
Mårtensson
S
S
Department of Clinical Chemistry, University Hospital, Lund, Sweden.
Lundblad
A
A
Hansson
G C
GC
Carlstedt
I
I
D000017:ABO Blood-Group System; D014408:Biomarkers, Tumor / Q000032:analysis; D002500:Centrifugation, Isopycnic; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D003106:Colon / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D006023:Glycoproteins / Q000652:urine*; D006801:Humans; D007413:Intestinal Mucosa / Q000276:immunology; D007983:Lewis Blood Group Antigens; D009077:Mucins / Q000652:urine*; D014547:Urinary Diversion
D000017:ABO Blood-Group System; D014408:Biomarkers, Tumor; D006023:Glycoproteins; D007983:Lewis Blood Group Antigens; D009077:Mucins