title: Human glucosamine-6-sulfatase cDNA reveals homology with steroid sulfatase.
abstract: Glucosamine-6-sulfatase is a lysosomal enzyme which degrades glycosaminoglycans and is deficient in mucopolysaccharidosis type IIID. Human liver contains two major active forms of glucosamine-6-sulfatase, form A which has a single 78 kDa polypeptide and form B which has two polypeptides of 48 kDa and 32 kDa. A 1761 base pair cDNA clone encoding the complete 48 kDa polypeptide of form B was isolated. Form A is shown to be processed to form B with the 48 kDa polypeptide C-terminal to the 32 kDa polypeptide, and it is shown that C-terminal processing is limited to a region of thirty amino acids. The glucosamine-6-sulfatase sequence reveals homology with steroid sulfatase, a microsomal enzyme.
authors.affiliation: Department of Chemical Pathology, Adelaide Children's Hospital, South Australia.
authors.forename: D A
authors.initials: DA
authors.lastname: Robertson
authors.forename: C
authors.initials: C
authors.lastname: Freeman
authors.forename: P V
authors.initials: PV
authors.lastname: Nelson
authors.forename: C P
authors.initials: CP
authors.lastname: Morris
authors.forename: J J
authors.initials: JJ
authors.lastname: Hopwood
mesh_terms: D000595:Amino Acid Sequence; D001192:Arylsulfatases / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D008970:Molecular Weight; D043266:Steryl-Sulfatase; D013429:Sulfatases / Q000235:genetics*
chemical_list: D004247:DNA; D013429:Sulfatases; D001192:Arylsulfatases; C018719:N-acetylglucosamine-6-sulfatase; D043266:Steryl-Sulfatase