Spontaneous association of purified major histocompatibility class I antigens with recipient cells after removal of detergent.
Affinity chromatography purified major histocompatibility class I antigens (MHC class I) from rat (RT-1) and mouse (H-2) became spontaneously associated with human lymphocytes after removal of the non-ionic detergent Triton X-100 by adsorption to Biobeads SM-2. No increased cell death was observed immediately after association with the new MHC class I antigen. However, after 20 h at 37 degrees C, cells with transferred antigen showed a lower viability than untreated cells. Long-time survival of the recipient cells improved if the MHC class I antigens were incorporated into liposomes. Lipids of differing compositions were investigated and the best long-term cell survival and the highest levels of transferred antigen were obtained with phosphatidylcholine liposomes. The transferred antigen exhibited time- and temperature-dependent removal from the cell surface and complement-mediated lysis of the cells indicated that some of the antigen was introduced into the membrane of the recipient cells.
Eriksson
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Cancer Biology Laboratory, State University Hospital, Copenhagen, Denmark.
D000818:Animals; D003165:Complement System Proteins; D003602:Cytotoxicity, Immunologic; D003902:Detergents; D006649:Histocompatibility Antigens; D015395:Histocompatibility Antigens Class I / Q000032:analysis*; D006801:Humans; D008081:Liposomes / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology*; D008565:Membrane Proteins / Q000276:immunology; D051379:Mice; D051381:Rats; D011919:Rats, Inbred Strains; D013501:Surface-Active Agents
D003902:Detergents; D006649:Histocompatibility Antigens; D015395:Histocompatibility Antigens Class I; D008081:Liposomes; D008565:Membrane Proteins; D013501:Surface-Active Agents; C038353:histocompatibility antigens RT, rat; D003165:Complement System Proteins