[The capacity of the cells of a human skin melanoma to be preserved long-term in an intact state in vitro].
Suspension of primary and metastatic skin melanoma cells isolated by the mechanical and enzymic methods was preserved in ampullae in the medium 199 at 4 degrees C. Native cells and cell preparations stained with trypan-blue solution and studied by the scanning electron microscopy method for 12, 20, 36 months have demonstrated the ability to keep their vitality for 3 years. Mechanism of such a "natural" conservation of cells is not yet clear. It may be assumed that the above cells are genetically stimulated mutants able under definite conditions to initiate an active proliferation in healthy tissues. The cells in a similar state may be a promising model in the experimental oncology.
Mosienko
M D
MD
Korovin
S I
SI
Naleskina
L A
LA
Katsen
A D
AD
Tolstopiatov
B A
BA
D002470:Cell Survival; D005615:Freezing; D006801:Humans; D008545:Melanoma / Q000473:pathology* / Q000556:secondary; D008855:Microscopy, Electron, Scanning; D011309:Preservation, Biological; D012878:Skin Neoplasms / Q000473:pathology* / Q000556:secondary; D013535:Suspensions; D013997:Time Factors; D014407:Tumor Cells, Cultured
D013535:Suspensions
Sposobnost' kletok melanomy kozhi cheloveka dlitel'no sokhraniat'sia v nepovrezhdennom sostoianii in vitro.