Modifications of a method for preparing retinal wholemounts for sectioning.
A method for embedding and sectioning retinal wholemounts is described. It employs a hydrophilic embedding agent, LR White, and coverslips inert to standard embedding resins. This simple technique, which has worked successfully on both enzymatically and autoradiographically labelled material, provides a means by which retinal wholemounts can be cut nearly parallel to the plane of the retinal layers. Previously prepared retinal wholemounts can also be successfully embedded and sectioned more than a year later by the method described.
Colello
R
R
Department of Human Anatomy, University of Oxford, United Kingdom.
D000180:Acrylic Resins; D000818:Animals; D066298:In Vitro Techniques; D008867:Microtomy / Q000379:methods*; D051381:Rats; D012160:Retina / Q000032:analysis* / Q000166:cytology
D000180:Acrylic Resins; C048707:LR white