Glucose flux and the redox state of pyridine dinucleotides in the rat lens.
The concentration of alpha-glycerophosphate (GP) appeared to increase and readily reached a new steady state in lenses incubated with KCN or under hyperglucosic condition. This increase can be explained by the change in NADH/NAD ratio under each condition. The relative ratio of pyridine dinucleotides (NADH/NAD) was calculated from equilibrium equations of two NAD-linked enzymes, lactate dehydrogenase (LDH) and alpha-glycerophosphate dehydrogenase (GPDH). The change in NADH/NAD ratio based on biochemical assays correlates well with that estimated from GPDH reaction. This indicates that measurements of pyridine dinucleotides (e.g. in vivo redox fluorometry) can be used to demonstrate lens metabolic status.
Cheng
H M
HM
Howe Laboratory of Ophthalmology, Harvard Medical School, Boston, MA 02114.
González
R G
RG
von Saltza
I
I
Chylack
L T
LT
Hutson
N J
NJ
D000818:Animals; D004099:Dihydroxyacetone Phosphate / Q000378:metabolism; D005636:Fructosephosphates / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D019298:Glucose-6-Phosphate; D005958:Glucosephosphates / Q000378:metabolism; D005993:Glycerolphosphate Dehydrogenase / Q000378:metabolism; D005994:Glycerophosphates / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D007908:Lens, Crystalline / Q000378:metabolism*; D009243:NAD / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D005636:Fructosephosphates; D005958:Glucosephosphates; D005994:Glycerophosphates; D009243:NAD; D019298:Glucose-6-Phosphate; D004099:Dihydroxyacetone Phosphate; C027618:fructose-6-phosphate; C029620:alpha-glycerophosphoric acid; D005993:Glycerolphosphate Dehydrogenase; D007770:L-Lactate Dehydrogenase; D005947:Glucose