title: Teratogenic antibody internalization by rat visceral yolk-sac endoderm in vitro: an ultrastructural colloidal gold tracer study.
abstract: Previous work from our laboratory has demonstrated that specific rabbit immunoglobulins G (IgG) against a glycoprotein antigen of rat kidney proximal tubule or a cross-reacting visceral yolk-sac endodermal cell antigen will induce abnormal embryonic development when they are injected into pregnant rats during the period of organogenesis. It has been proposed that these antibodies may induce embryopathy by interfering with functions of the visceral yolk-sac placenta, an important organ providing nutrients to the embryo at this stage of development. In order to gain some insight into the underlying pathogenic mechanism(s) in which specific teratogenic IgG may interfere with visceral yolk-sac functions, we examined the uptake of these teratogenic IgG by the visceral yolk-sac endodermal cells at the electron microscopic level. The results demonstrated that teratogenic rabbit IgG specifically localized on the fuzzy coat of the external apical cell membrane of the visceral yolk-sac endoderm at the intermicrovillous region. Within 5 min, the IgG were rapidly internalized via coated pits and micropinocytic vesicles. Within 30 min, an increasing proportion of gold particles appeared within uncoated vesicles or vacuoles of various sizes; most of the gold particles were in close proximity to the inner membranous lining of the vesicles. Similar findings were observed after 1- or 2-hr incubation. After 24- to 48-hr culture, however, the gold particles appeared to have dissociated from the inner surface of the vesicle membrane.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Anatomy, UMDNJ-New Jersey Medical School, Newark 07103.
authors.forename: C C
authors.initials: CC
authors.lastname: Leung
authors.forename: C L
authors.initials: CL
authors.lastname: Yan
authors.forename: B
authors.initials: B
authors.lastname: Cheewatrakoolpong
mesh_terms: D000818:Animals; D000906:Antibodies / Q000032:analysis* / Q000276:immunology / Q000493:pharmacokinetics; D004707:Endoderm / Q000032:analysis / Q000378:metabolism / Q000648:ultrastructure*; D004848:Epithelium / Q000032:analysis / Q000378:metabolism / Q000648:ultrastructure; D005260:Female; D006046:Gold; D008297:Male; D008854:Microscopy, Electron / Q000379:methods; D011247:Pregnancy; D051381:Rats; D015017:Yolk Sac / Q000032:analysis / Q000166:cytology* / Q000378:metabolism
chemical_list: D000906:Antibodies; D006046:Gold