Expression of H-2K and H-2D genes by PYS-2 teratocarcinoma cells.
The parietal yolk-sac carcinoma cell line PYS-2, which has been described to synthesize a trace amount of H-2 antigens, was shown to express low levels of Kb- and/or Db-specific antigens by flow cytometric analysis. Further studies of H-2 cDNA clones from PYS-2 cDNA library revealed 8 out of 10 clones were identified as classical H-2Kb and 2 were H-2Db, which confirmed the result from the immunofluorescent analysis. However, we have not isolated Qa, Tla or other embryonic class I cDNAs from our PYS-2 library.
Nagata
T
T
Department of Microbial Genetics, Osaka University, Japan.
Morita
T
T
Nozaki
M
M
Matsushiro
A
A
D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D005434:Flow Cytometry; D005786:Gene Expression Regulation; D006183:H-2 Antigens / Q000235:genetics*; D066298:In Vitro Techniques; D051379:Mice; D008969:Molecular Sequence Data; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D013724:Teratoma / Q000235:genetics; D014407:Tumor Cells, Cultured
D006183:H-2 Antigens; D012333:RNA, Messenger