In vitro model for Campylobacter pylori adherence properties.
The adherence of 12 strains of Campylobacter pylori was studied on four cell lines. Immunofluorescence and scanning and transmission electron microscopy were used to visualize the bacteria. A heavy adherence to the epithelial cell line HEp-2 and to the intestinal cell line Int-407 was noted. By transmission electron microscopy, a close association between bacteria and cells in the form of cup-like structures was observed, but pedestals were not present.
Neman-Simha
V
V
Laboratoire de Bactériologie, Hôpital des Enfants, Bordeaux, France.
Mégraud
F
F
D001422:Bacterial Adhesion; D002167:Campylobacter / Q000502:physiology* / Q000648:ultrastructure; D002460:Cell Line; D006801:Humans; D066298:In Vitro Techniques; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning