title: Food intake suppression in rats by a substance isolated from human feces.
abstract: A substance which suppresses food intake was isolated from human feces. This substance was extracted and partially purified using Sephacryl S-200 column chromatography and a DEAE-Sephadex A-25 column chromatography. To assess the biological activity, rats were subjected to various applications. The intraperitoneal administration and the intracerebroventricular microinfusion of this substance suppresses short- and long-term food intake. The central infusion suppresses feeding with a potency over 1000 times that the peripheral administration. The central infusion of the heat-treated substance was without effect. These results suggest that a substance present in human feces (probably of a proteic nature) suppresses food intake in rats, at the level of the central nervous system.
authors.affiliation: Department of Physiology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
authors.forename: C R
authors.initials: CR
authors.lastname: Plata-Salamán
authors.forename: T
authors.initials: T
authors.lastname: Yamamoto
authors.forename: Y
authors.initials: Y
authors.lastname: Oomura
authors.forename: N
authors.initials: N
authors.lastname: Shimizu
authors.forename: T
authors.initials: T
authors.lastname: Sakata
mesh_terms: D000818:Animals; D001067:Appetite Depressants / Q000302:isolation & purification*; D004435:Eating; D005215:Fasting; D005243:Feces / Q000032:analysis*; D006801:Humans; D007026:Hypothalamic Area, Lateral / Q000502:physiology; D008297:Male; D009842:Oligopeptides / Q000302:isolation & purification*; D011761:Pyrrolidonecarboxylic Acid / Q000031:analogs & derivatives; D051381:Rats; D014697:Ventromedial Hypothalamic Nucleus / Q000502:physiology
chemical_list: D001067:Appetite Depressants; D009842:Oligopeptides; C023866:pyroglutamyl-histidyl-glycine; D011761:Pyrrolidonecarboxylic Acid