Heparin binding to normal and abnormal platelets.
The binding of porcine heparin to human platelets was studied using [3H] heparin and various concentrations of unlabeled heparin. The binding was readily reversible and included saturable (Kd approximately 1.5 mg/L, R approximately 500 mg/10(15) cells) and unsaturable components. Approximately normal binding also occurred to platelets from patients with the Bernard-Soulier syndrome and Glanzmann's thrombasthenia. Competitive inhibition studies were performed with a variety of sulfated polysaccharides. Inhibitory potency correlated with charge density but not with the fundamental sugar composition of the tested compounds.
Horne
M K
MK
Hematology Section, National Institutes of Health, Bethesda, MD 20892.
D000818:Animals; D001606:Bernard-Soulier Syndrome / Q000097:blood; D001667:Binding, Competitive; D001792:Blood Platelets / Q000378:metabolism*; D006493:Heparin / Q000378:metabolism*; D006801:Humans; D013552:Swine; D013915:Thrombasthenia / Q000097:blood
D006493:Heparin