Cloning and sequence analysis of cDNA for corticotropin-releasing factor precursor from the teleost fish Catostomus commersoni.
The sequence of a cDNA encoding the corticotropin-releasing factor precursor has been identified by screening lambda gt11 libraries constructed from poly(A)+ RNA of the hypothalamic region of the white sucker Catostomus commersoni brain with synthetic oligonucleotide probes deduced from the sequence of the rat corticotropin-releasing factor. The amino acid sequence of corticotropin-releasing factor of the sucker is strikingly conserved when compared to its counterpart from rat and differs only in two positions at the carboxyl terminus; in contrast, there is little similarity between their cryptic regions.
Okawara
Y
Y
Institut für Zellbiochemie und klinische Neurobiologie, Universitäts-Krankenhaus Eppendorf, Hamburg, Federal Republic of Germany.
Morley
S D
SD
Burzio
L O
LO
Zwiers
H
H
Lederis
K
K
Richter
D
D
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003062:Codon; D004247:DNA / Q000235:genetics*; D005399:Fishes / Q000235:genetics*; D005796:Genes; D008969:Molecular Sequence Data; D015183:Restriction Mapping
D003062:Codon; D004247:DNA