Xeroderma pigmentosum group E cells lack a nuclear factor that binds to damaged DNA.
The disease xeroderma pigmentosum is characterized by deficient repair of damaged DNA. Fusions of cells from different patients have defined nine genetic complementation groups (A through I), implying that DNA repair in humans involves multiple gene products. In this report, an extension of the gel electrophoresis binding assay was used to identify at least one nuclear factor that (i) bound to DNA damaged by ultraviolet radiation or the antitumor drug cisplatin, but (ii) was notably absent in cells from complementation group E. Therefore, the factor appears to participate in a versatile DNA repair pathway at the stage of binding and recognition.
Chu
G
G
Department of Medicine, Stanford University School of Medicine, CA 94305.
Chang
E
E
D002460:Cell Line; D002945:Cisplatin; D004249:DNA Damage; D015342:DNA Probes; D004260:DNA Repair; D004268:DNA-Binding Proteins / Q000235:genetics*; D005816:Genetic Complementation Test; D006367:HeLa Cells; D009687:Nuclear Proteins / Q000235:genetics*; D014466:Ultraviolet Rays; D014983:Xeroderma Pigmentosum / Q000235:genetics*
D015342:DNA Probes; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D002945:Cisplatin