Production and characterization of monoclonal antibodies to the macrocyclic trichothecene roridin A.
Two murine monoclonal antibodies to the macrocyclic trichothecene roridin A are described. Screening for antibody production was performed on absorbed anti-mouse immunoglobulin serum as double-antibody solid phase, and further characterization was done on affinity-purified anti-mouse IgG serum. The antibodies, designated 5G11 and 4H10, had affinity constants for roridin A of 9.25 X 10(7) and 1.7 X 10(7) liters/mol, respectively. In monoclonal antibody-based direct enzyme immunoassays, these IgG1 antibodies had detection limits for roridin A of 0.4 ng/ml (0.02 ng per assay) and 1.8 ng/ml (0.09 ng per assay), respectively. Both antibodies were most specific for the tested macrocyclic trichothecenes. The relative cross-reactivities of antibody 5G11 with roridin A, roridin J, verrucarin A, satratoxin G, and satratoxin H were 100.0, 43.8, 16.7, 3.7, and 18.9%, respectively; for antibody 4H10 they were 100.0, 6.3, 64.0, 4.4, and 4.9%, respectively.
Hack
R
R
Institute for Hygiene and Technology of Food of Animal Origin, Veterinary Faculty, University of Munich, Federal Republic of Germany.
Märtlbauer
E
E
Terplan
G
G
D000818:Animals; D000900:Anti-Bacterial Agents / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000096:biosynthesis*; D000918:Antibody Specificity; D055598:Chemical Phenomena; D002621:Chemistry; D003429:Cross Reactions; D051379:Mice; D009183:Mycotoxins / Q000276:immunology*; D014255:Trichothecenes / Q000276:immunology
D000900:Anti-Bacterial Agents; D000911:Antibodies, Monoclonal; D009183:Mycotoxins; D014255:Trichothecenes; C038849:roridin A