title: Clonogenic growth of acute non-lymphocytic leukemia cells in serum-free medium.
abstract: We devised a serum-free medium for growth of leukemic colony-forming units (CFU-L), enriched with albumin, transferrin, lipids, insulin, hydrocortisone and oligoelements. Blast cells from 15 patients affected by acute non-lymphocytic leukemia were grown in this medium in the presence of human placental conditioned medium obtained under serum-free conditions (sfHPCM). Their clonogenic growth was comparable with that obtained in a serum-containing system. Furthermore, when serum-free cultures were carried out in absence of sfHPCM, either CFU-L growth was prevented or, if clones were obtained, the cultures showed a marked decrease in clonogenicity, indicating their strict dependence on growth factors.
authors.affiliation: Università degli Studi di Firenze, Policlinico di Careggi, Italy.
authors.forename: P A
authors.initials: PA
authors.lastname: Bernabei
authors.forename: V
authors.initials: V
authors.lastname: Santini
authors.forename: O
authors.initials: O
authors.lastname: Dal Pozzo
authors.forename: R
authors.initials: R
authors.lastname: Bezzini
authors.forename: V
authors.initials: V
authors.lastname: Gattei
authors.forename: R
authors.initials: R
authors.lastname: Saccardi
authors.forename: G
authors.initials: G
authors.lastname: Rombolà
authors.forename: P R
authors.initials: PR
authors.lastname: Ferrini
mesh_terms: D000328:Adult; D001769:Blood; D002455:Cell Division; D002999:Clone Cells; D003470:Culture Media; D006133:Growth Substances; D006412:Hematopoietic Stem Cells / Q000473:pathology; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000473:pathology*; D008055:Lipids; D010920:Placenta; D014407:Tumor Cells, Cultured
chemical_list: D003470:Culture Media; D006133:Growth Substances; D008055:Lipids