Analysis of commercial samples of acridine orange using high-performance liquid chromatography.
We analyzed commercial samples of acridine orange using reverse-phase high-performance liquid chromatography. The mobile phase of 90:10:2.5 acetonitrile:de-ionized water:pentane sulfonic acid gave baseline separations of components of acridine orange samples in 15 min. Many of the samples were fairly homogeneous; the absorbance due to the acridine orange component ranged from 83-97%. Among the 11 samples tested, one was not acridine orange, reputedly different samples were identical, and the principal component of one sample was not acridine orange.
Paulsen
R D
RD
Department of Anatomical Sciences and Neurobiology, University of Louisville, Kentucky 40292.
Nettleton
G S
GS
D000097:Acetonitriles / Q000032:analysis; D000165:Acridine Orange / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D013056:Spectrophotometry, Ultraviolet; D013451:Sulfonic Acids / Q000032:analysis; D014867:Water / Q000032:analysis
D000097:Acetonitriles; D013451:Sulfonic Acids; D014867:Water; D000165:Acridine Orange; C032159:acetonitrile