Rapid separation and purification of oligonucleotides by high-performance capillary gel electrophoresis.
Picomole amounts of oligodeoxynucleotides [polydeoxyadenylic acids, (dA)40-60] were baseline resolved and analyzed in less than 8 min by high-performance capillary electrophoresis with polyacrylamide gels. In addition, fast analysis of a crude 70-mer oligodeoxynucleotide and a slab gel-purified 99-mer oligodeoxynucleotide was accomplished, demonstrating the ability of high-performance capillary electrophoresis to characterize rapidly synthesized oligonucleotides. Besides analytical separations, 800 ng of a primer (20-mer) was isolated in less than 20 min. The purified species was collected in water and subsequently used as a probe in a standard dot-blot analysis. The use of high-performance capillary electrophoresis for the analysis and purification of a variety of biopolymers is simple, rapid, and has the potential for automation.
Cohen
A S
AS
Barnett Institute, Northeastern University, Boston, MA 02115.
Najarian
D R
DR
Paulus
A
A
Guttman
A
A
Smith
J A
JA
Karger
B L
BL
D004586:Electrophoresis / Q000379:methods; D008832:Microchemistry / Q000379:methods; D009843:Oligoribonucleotides / Q000302:isolation & purification*; D013997:Time Factors
D009843:Oligoribonucleotides