title: Localization of 28 kDa calbindin in human odontoblasts.
abstract: The presence of 28 kDa calbindin in human odontoblasts was studied by use of specific antibodies raised against chick duodenal 28 kDa calbindin, in immunofluorescence, immuno-peroxidase, and electron-microscopic labelling experiments. The calbindin-like protein was detected mainly in the cytoplasm of odontoblast cell bodies, in their processes and occasionally in their nuclei. Correspondingly, at the ultrastructural level, immunoreactive material was associated with the cytosol, microfilaments and cilia. These findings suggest that human odontoblasts express a 28 kDa vitamin D-dependent calcium-binding protein, unlike those of rats and mice in which ameloblasts are the only cells immunoreactive for the protein.
authors.affiliation: Laboratoire d'Histophysiologie et de Pathologie des Tissus Dentaires, CNRS (UA 244), Faculté d'Odontologie et CMEABG, Lyon, France.
authors.forename: H
authors.initials: H
authors.lastname: Magloire
authors.forename: A
authors.initials: A
authors.lastname: Joffre
authors.forename: J
authors.initials: J
authors.lastname: Azerad
authors.forename: D E
authors.initials: DE
authors.lastname: Lawson
mesh_terms: D000818:Animals; D064026:Calbindins; D002467:Cell Nucleus / Q000032:analysis / Q000648:ultrastructure; D003593:Cytoplasm / Q000032:analysis / Q000648:ultrastructure; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D008815:Mice, Inbred Strains; D008854:Microscopy, Electron; D008963:Molar / Q000032:analysis / Q000648:ultrastructure; D009804:Odontoblasts / Q000032:analysis* / Q000648:ultrastructure; D064030:S100 Calcium Binding Protein G / Q000032:analysis*; D014070:Tooth / Q000032:analysis / Q000648:ultrastructure
chemical_list: D064026:Calbindins; D064030:S100 Calcium Binding Protein G