title: Gastric diversion or pylorus ligation for gastric mucosal integrity and acid secretion studies in the rat?
abstract: The advantages of gastric diversion over pylorus ligation in rat gastric mucosal integrity and acid secretion studies over 6 hr were investigated. Mucosal injury developed in 80% of pylorus-ligation controls. Atropine (5 mg/kg) or cimetidine (40 mg/kg) had no effect on this injury (2.9 mm2 +/- 0.9 and 2.8 mm2 +/- 0.7, respectively, vs 3.1 mm2 +/- 1, mean +/- SEM, N = 10); however vagotomy increased it (13.7 mm2 +/- 1.5, mean +/- SEM, N = 10, P less than 0.001). Gastric diversion produced no mucosal injury. Pylorus-ligation H+ output was higher than that of gastric diversion (390.5 mumol +/- 54.8 vs 61 mumol +/- 2.5, mean +/- SEM, N = 10, P less than 0.001). Cimetidine (40 mg/kg) depressed H+ output of gastric diversion (21.3 mumol +/- 1.2 vs 61 mumol +/- 2.5, mean +/- SEM, N = 10, P less than 0.001), but not of pylorus ligation (424 mumol +/- 74.2 vs 390.5 mumol +/- 54.8, mean +/- SEM, N = 10). Vagotomy or atropine depressed pylorus-ligation H+ output (P less than 0.001), but each allowed an output (36.6 mumol +/- 5.5 and 120 mumol +/- 29, respectively, mean +/- SEM, N = 10) significantly (P less than 0.001) higher than that associated with it in gastric diversion (16 mumol +/- 1.4 and 17.1 mumol +/- 1.6, respectively, mean +/- SEM, N = 10). This study demonstrates that in the rat pylorus ligation, in contrast to gastric diversion, injures the gastric mucosa, stimulates H+ secretion, and overshadows the efficacy of antisecretory agents.
authors.affiliation: University Department of Surgery Royal Infirmary, Glasgow, U.K.
authors.forename: A S
authors.initials: AS
authors.lastname: Salim
mesh_terms: D000818:Animals; D001285:Atropine / Q000494:pharmacology; D005260:Female; D005744:Gastric Acid / Q000378:metabolism*; D005753:Gastric Mucosa / Q000293:injuries* / Q000502:physiology; D008026:Ligation; D008297:Male; D011708:Pylorus / Q000601:surgery*; D051381:Rats; D011919:Rats, Inbred Strains; D013048:Specimen Handling / Q000379:methods; D014628:Vagotomy
chemical_list: D001285:Atropine