Infection of the SCID-hu mouse by HIV-1.
SCID-hu mice with human fetal thymic or lymph node implants were inoculated with the cloned human immunodeficiency virus-1 isolate, HIV-1JR-CSF. In a time- and dose-dependent fashion, viral replication spread within the human lymphoid organs. Combination immunohistochemistry and in situ hybridization revealed only viral RNA transcripts in most infected cells, but some cells had both detectable viral transcripts and viral protein. Infected cells were always more apparent in the medulla than in the cortex of the thymus. These studies demonstrate that an acute infection of human lymphoid organs with HIV-1 can be followed in the SCID-hu mouse.
Namikawa
R
R
Department of Pathology, Stanford University School of Medicine, CA 94305.
Kaneshima
H
H
Lieberman
M
M
Weissman
I L
IL
McCune
J M
JM
D000163:Acquired Immunodeficiency Syndrome; D000818:Animals; D002678:Chimera; D004195:Disease Models, Animal; D006678:HIV / Q000235:genetics / Q000502:physiology*; D006801:Humans; D007150:Immunohistochemistry; D008198:Lymph Nodes / Q000382:microbiology / Q000637:transplantation; D051379:Mice; D008817:Mice, Mutant Strains; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000235:genetics; D013950:Thymus Gland / Q000382:microbiology / Q000637:transplantation; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000096:biosynthesis; D014779:Virus Replication
D012367:RNA, Viral; D014764:Viral Proteins