Coagglutination of Vibrio cholerae, Vibrio mimicus, and Vibrio vulnificus with anti-flagellar monoclonal antibody.
Monoclonal antibodies (MAbs) with serological activity for purified flagellar (H) core protein prepared from Vibrio cholerae were identified by enzyme-linked immunosorbent assay. Four of these MAbs reacted with the flagella of V. cholerae and V. mimicus exclusively, while eight MAbs reacted with at least 1 of 30 heterologous Vibrio species tested by enzyme-linked immunosorbent assay or coagglutination. It appears that V. cholerae and V. mimicus express similar, if not identical, H determinants unique to these two Vibrio species. Staphylococcus aureus cells or latex beads armed with the four species-specific MAbs coagglutinated each of 47 isolates identified bacteriologically as V. cholerae or V. mimicus from among 103 Vibrio isolates tested. One coagglutination reagent armed with anti-V. vulnificus H MAb exhibited species specificity in that only V. vulnificus cells were coagglutinated from among the 31 Vibrio species examined. This reagent coagglutinated 20 isolates identified bacteriologically as V. vulnificus in a serological survey. MAb coagglutination reagents offer a rapid, specific, and economical alternative to the classical bacteriological approach to identify the human pathogens V. cholerae, V. mimicus, and V. vulnificus.
Simonson
J G
JG
Department of Microbiology, Louisiana State University, Baton Rouge 70803.
Siebeling
R J
RJ
D000371:Agglutination; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005407:Flagella / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D013045:Species Specificity; D014733:Vibrio / Q000145:classification / Q000276:immunology*; D014734:Vibrio cholerae / Q000276:immunology*
D000911:Antibodies, Monoclonal