title: Disruption of the proto-oncogene int-2 in mouse embryo-derived stem cells: a general strategy for targeting mutations to non-selectable genes.
abstract: Gene targeting--homologous recombination of DNA sequences residing in the chromosome with newly introduced DNA sequences--in mouse embryo-derived stem cells promises to provide a means to generate mice of any desired genotype. We describe a positive nd negative selection procedure that enriches 2,000-fold for those cells that contain a targeted mutation. The procedure was applied to the isolation of hprt- and int-2- mutants, but it should be applicable to any gene.
authors.affiliation: Howard Hughes Medical Institute, Department of Biology, University of Utah, Salt Lake City 84112.
authors.forename: S L
authors.initials: SL
authors.lastname: Mansour
authors.forename: K R
authors.initials: KR
authors.lastname: Thomas
authors.forename: M R
authors.initials: MR
authors.lastname: Capecchi
mesh_terms: D000818:Animals; D002999:Clone Cells; D051379:Mice; D008822:Mice, Transgenic; D009154:Mutation; D011519:Proto-Oncogenes; D015183:Restriction Mapping; D013234:Stem Cells; D014162:Transfection