Correlation between the surface hydrophobicities and elution orders of elapid neurotoxins and cardiotoxins on hydrophobic-interaction high-performance liquid chromatography.
Hydrophilic and hydrophobic regions were predicted for Elapid neuro- and cardiotoxins. The contribution of these regions to the retention times of neuro- and cardiotoxins on hydrophobic-interaction HPLC was assessed from the known surface accessibilities of amino acid side-chains within these regions. Differences in retention times between neuro- and cardiotoxins on hydrophobic-interaction HPLC could be attributed to differences in hydrophobicity of regions 6-12 and 22-26 between these two types of toxins. Smaller differences in retention times between cardiotoxins were due to the variable hydrophobicities of regions 1-4 and 26-36.
Osthoff
G
G
National Chemical Research Laboratory, Council for Scientific and Industrial Research, Pretoria, Republic of South Africa.
Louw
A I
AI
Reinecke
C J
CJ
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D002942:Circular Dichroism; D004179:Cobra Cardiotoxin Proteins / Q000032:analysis*; D004546:Elapid Venoms / Q000032:analysis*; D009498:Neurotoxins / Q000032:analysis*; D012995:Solubility; D013499:Surface Properties
D004179:Cobra Cardiotoxin Proteins; D004546:Elapid Venoms; D009498:Neurotoxins