Inhibition of platelet aggregation by manoalide: preliminary results.
Manoalide (MND), a sesterterpenoid first isolated from the marine sponge Luffariela variabilis and later synthesized by Japanese chemists, exhibits anti-inflammatory activity and directly inactivates bee and snake venom phospholipase A2. We investigated the effects of MND on platelet aggregation induced by PAF-acether, arachidonic acid (AA), ADP and thrombin. Rabbit platelet aggregation was inhibited by MND in a dose-dependent manner. MND also inhibited the aggregation induced by AA and ADP but not that induced by thrombin. Since this marine natural product is also a potent inhibitor of lipoxygenase in human polymorphonuclear neutrophils, MND appears to be a useful tool for determining the role of phospholipase A2 in biological processes.
Freitas
J C
JC
Departmento de Fisiologia Geral, Universidade de São Paulo, Brasil.
Rodrigue
F
F
Lavaud
P
P
Mencia-Huerta
J M
JM
Braquet
P
P
D000818:Animals; D001241:Aspirin / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D010972:Platelet Activating Factor / Q000494:pharmacology; D010974:Platelet Aggregation / Q000187:drug effects*; D011817:Rabbits; D013729:Terpenes / Q000008:administration & dosage / Q000494:pharmacology*
D010972:Platelet Activating Factor; D013729:Terpenes; C045673:manoalide; D001241:Aspirin