title: Cystatin superfamily. Evidence that family II cystatin genes are evolutionarily related to family III cystatin genes.
abstract: Human saliva contains at least three molecular species of cystatin S-type cysteine proteinase inhibitor (cystatin S, cystatin SN and cystatin SA), which have similar but distinct amino-acid sequences. The nucleotide sequences of the CST 1 gene for cystatin SN and the CST 2 gene for cystatin SA are highly homologous to each other and to the corresponding regions of the cDNA for cystatin C and the EcoRI-PstI fragment from the cystatin C gene. Three cystatin-like domains in the kininogen gene and the salivary cystatin genes share the same gene organizations. These data demonstrate that family II cystatin genes are evolutionarily related to family III cystatin genes.
authors.affiliation: Department of Oral Biochemistry, School of Dentistry at Niigata, Nippon Dental University.
authors.forename: E
authors.initials: E
authors.lastname: Saitoh
authors.forename: S
authors.initials: S
authors.lastname: Isemura
authors.forename: K
authors.initials: K
authors.lastname: Sanada
authors.forename: H S
authors.initials: HS
authors.lastname: Kim
authors.forename: O
authors.initials: O
authors.lastname: Smithies
authors.forename: N
authors.initials: N
authors.lastname: Maeda
mesh_terms: D000595:Amino Acid Sequence; D005075:Biological Evolution; D015891:Cystatins; D015853:Cysteine Proteinase Inhibitors; D004247:DNA / Q000235:genetics; D006801:Humans; D007525:Isoelectric Focusing; D008969:Molecular Sequence Data; D005810:Multigene Family; D012463:Saliva / Q000032:analysis*; D055333:Salivary Cystatins; D012471:Salivary Proteins and Peptides / Q000235:genetics*
chemical_list: C525171:CST1 protein, human; C525172:CST2 protein, human; C525173:CST4 protein, human; D015891:Cystatins; D015853:Cysteine Proteinase Inhibitors; D055333:Salivary Cystatins; D012471:Salivary Proteins and Peptides; D004247:DNA