Subcellular distribution of protein kinase C in rat adrenal glomerulosa cells.
With the aid of a synthetic nonapeptide which is a selective substrate for protein kinase C the activity of this enzyme was determined in the crude cytosolic and particulate fractions of rat adrenal glomerulosa cells. When the cells were sonicated in the presence of Ca2+ chelators 65 per cent of their total protein kinase C activity was found in the cytosolic extract. The treatment of cells with angiotensin II under conditions where the maximal stimulation of inositol-lipid hydrolysis was observed did not cause a statistically significant change in the apparent subcellular distribution of protein kinase C. However, when the cytosolic extract was prepared in the presence of Ca2+ the protein kinase C activity was recovered nearly exclusively from the particulate fraction.
Faragó
A
A
1st Institute of Biochemistry, Semmelweis University Medical School, Budapest, Hungary.
Seprõdi
J
J
Spät
A
A
D000804:Angiotensin II / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D003600:Cytosol / Q000201:enzymology; D007976:Leupeptins / Q000494:pharmacology; D010664:Phenylmethylsulfonyl Fluoride / Q000494:pharmacology; D010743:Phospholipids / Q000494:pharmacology; D011493:Protein Kinase C / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D013347:Subcellular Fractions / Q000201:enzymology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D015384:Zona Glomerulosa / Q000187:drug effects / Q000201:enzymology* / Q000648:ultrastructure
D007976:Leupeptins; D010743:Phospholipids; D000804:Angiotensin II; D010664:Phenylmethylsulfonyl Fluoride; D011493:Protein Kinase C; C032854:leupeptin; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium