Auto-anti-idiotypic antibodies in mice hyperimmunized with a chemically induced bladder carcinoma.
Purified immunoglobulin from the serum of mice hyperimmunized with syngeneic tumour cells was compared with a monoclonal anti-idiotope antibody, obtained by immunizing mice with antibody to the same tumour. Both the immunoglobulin and the monoclonal antibody were specifically immunogenic in syngeneic (BALB/c) mice as tested by in vitro and in vivo assays of cell-mediated immunity. In both cases the reactivity was absent in immunoglobulin heavy chain gene complex-1-(Igh) allotype congenic (CB.20) mice, indicating Igh-restriction of immunogenicity. The active material in hyperimmune serum thus has the properties of auto-anti-idiotypic antibody, complementary to T cell idiotopes.
Greer
J M
JM
Department of Microbiology, University of Queensland, St Lucia, Australia.
Halliday
W J
WJ
D000818:Animals; D001323:Autoantibodies / Q000276:immunology*; D005260:Female; D007132:Immunoglobulin Isotypes / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008815:Mice, Inbred Strains; D001749:Urinary Bladder Neoplasms / Q000139:chemically induced / Q000276:immunology*
D001323:Autoantibodies; D007132:Immunoglobulin Isotypes