title: Analysis of mutant frequency curves and survival curves applied to the AL hybrid cell system.
abstract: A model is presented for the statistical analysis of survival curves and mutant frequency curves for a hybrid cell system. The derivation of the model is given in the Appendix, and depends on simple assumptions about the distribution of insults, their repair, and the loss of a marker that is not rescued. A single formula (5) is found which relates a survival curve to the mutant frequency curve, i. e., the response curve for production of mutants per 10(5) survivors induced by a mutagen. The analysis is applied to loss of the a1 gene in AL-J1 hybrid cells submitted to Cesium gamma-rays. Previous experimental data using X-rays was reported by Waldren et al. (1986: Proc. Natl. Acad. Sci, USA 83, 4839.) Also, a derived formula (10), which predicts the probability that in a surviving cell a marker is lost and not rescued, will form the basis for testing the validity of the model in the future using new experimental data.
authors.affiliation: Johns Hopkins Medical Institutions, Baltimore, MD 21205.
authors.forename: R
authors.initials: R
authors.lastname: Parker
authors.forename: C
authors.initials: C
authors.lastname: Waldren
authors.forename: T K
authors.initials: TK
authors.lastname: Hei
authors.forename: D F
authors.initials: DF
authors.lastname: Wong
authors.forename: T T
authors.initials: TT
authors.lastname: Puck
authors.forename: T L
authors.initials: TL
authors.lastname: Puck
mesh_terms: D000704:Analysis of Variance; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays; D006822:Hybrid Cells / Q000528:radiation effects*; D008957:Models, Genetic; D009154:Mutation; D011336:Probability