A 15,000-Mr protein proteolytically derived from vitellogenin within oocyte of Perinereis cultrifera (polychaete annelid). Identification, purification and partial characterization.
It has been shown previously in our laboratory that, in Perinereis cultrifera, the four mature vitellin subunits (Mr 98,000, 22,000, 20,000, 16,000) are proteolytically derived within the oocyte from a single extraoocytic precursor, vitellogenin, with an apparent Mr (176,000) higher by 20,000 than the sum of the Mr of the four end products. In this report, it is shown that a 15,000-Mr protein, designated as P15, not only accumulates in maturing oocytes but also originates from outside these cells similarly to vitellin. Moreover in vivo labelling experiments indicate that the appearance of P15 occurs after vitellogenin enters the oocyte, concurrently with the appearance of the lower-Mr fragments characteristic of vitellin. From these data, it is concluded that P15 most likely represents a vitellogenin-derived protein which is generated within the oocytes during the transformation of vitellogenin into vitellin. This conclusion is further supported by the additional finding that P15 immunologically cross-reacts with vitellogenin but not with mature vitellin. P15 has been purified to homogeneity from the soluble protein fraction of submature oocytes and partially characterized. The 15,000-Mr protein exists in a monomeric form with a pI of about 7.7. Unlike vitellin, P15 does not contain significant amounts of lipid or carbohydrate and has a low absorbance at 280 nm. The amino acid composition of the purified protein is also presented.
Baert
J L
JL
Laboratoire d'Endocrinologie des Invertébrés, Unité associée 148 du Centre National de la Recherche Scientifique, Université de Lille-Flandres-Artois, Villeneuve-d'Ascq, France.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D007106:Immune Sera; D046911:Macromolecular Substances; D008970:Molecular Weight; D009865:Oocytes / Q000032:analysis*; D011077:Polychaeta; D014819:Vitellogenins / Q000302:isolation & purification
D000596:Amino Acids; D007106:Immune Sera; D046911:Macromolecular Substances; D014819:Vitellogenins