A simple and reliable method for monitoring platelet serotonin levels in rats.
A simple and reliable method for individual monitoring of platelet serotonin in rats is developed. Platelet-rich plasma is prepared under standardized conditions from 1 mL of venous blood and the platelets are quantitatively separated by a highly reproducible procedure. Platelet serotonin content is determined spectrophotofluorometrically and the results are comparatively expressed per standardized platelet rich plasma sample (1.01 +/- 0.18 microgram), per mg of platelet protein (1.57 +/- 0.15 microgram) and per 10(9) platelets (2.16 +/- 0.38 micrograms). Normal distribution of platelet serotonin levels in a sample of 338 animals is shown. By use of the described method, the intraindividual stability of platelet serotonin concentration in rats is demonstrated for the first time.
Jernej
B
B
Department of Experimental Biology, Ruder Bosković Institute, Zagreb, Yugoslavia.
Cicin-Sain
L
L
Iskrić
S
S
D000818:Animals; D001792:Blood Platelets / Q000032:analysis / Q000166:cytology; D001800:Blood Specimen Collection / Q000379:methods; D002469:Cell Separation / Q000379:methods; D008297:Male; D008991:Monitoring, Physiologic / Q000379:methods; D051381:Rats; D011919:Rats, Inbred Strains; D012701:Serotonin / Q000097:blood*; D013050:Spectrometry, Fluorescence / Q000379:methods
D012701:Serotonin