Mass spectrometry of hexosamine containing oligosaccharides as permethylated N-trifluoroacetyl derivatives.
Permethylated N-trifluoroacetyl hexosamine containing oligosaccharide alditols are favorable for analysis by mass spectrometry. Electron impact and fast atom bombardment mass spectra of these derivatives are characterized by strong primary and secondary fragments in which a positive charge is localized on the N-trifluoroacetyl hexosamine residue. From the mass spectra the monosaccharide sequence and the position(s) of substitution of a N-trifluoroacetyl hexosamine can be determined.
Nilsson
B
B
BioCarb AB, Lund, Sweden.
D002240:Carbohydrate Sequence; D008401:Gas Chromatography-Mass Spectrometry; D006595:Hexosamines; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009844:Oligosaccharides; D014269:Trifluoroacetic Acid
D006595:Hexosamines; D009844:Oligosaccharides; D014269:Trifluoroacetic Acid