Activated charcoal diminishes the lot difference of fetal bovine sera in erythroid colony formation of human bone marrow cells.
Using normal bone marrow as target cells, we assayed the colony-forming efficiency of early and late erythroid progenitor cells and granulocyte-macrophage progenitor cells using several different lots of fetal bovine serum (FBS). There was a marked difference in the ability of these sera to support colony formation, particularly in erythroid colony assays. When adsorbed by activated charcoal, all these sera supported erythroid colony formation more efficiently than before adsorption. There was no significant effect of charcoal adsorption of FBS on granulocyte-macrophage colony formation. Gel-filtration study showed that charcoal adsorption diminished low-molecular-weight fractions by less than 5000 Da. The inhibitory activity of this fraction was heat labile and Pronase sensitive. Concentrated samples obtained from these fractions inhibited erythroid colony formation in a dose-dependent manner. These results suggest that low-molecular-weight inhibitors that are relatively specific to erythropoiesis play a critical role in the lot differences of FBS for erythroid colony formation.
Murate
T
T
First Department of Internal Medicine, Nagoya University School of Medicine, Japan.
Hotta
T
T
Goto
S
S
Ichikawa
A
A
Inoue
C
C
Kaneda
T
T
D000327:Adsorption; D000818:Animals; D001854:Bone Marrow Cells; D002417:Cattle; D002606:Charcoal; D003114:Colony-Forming Units Assay; D004900:Erythroblasts / Q000166:cytology; D004920:Erythropoiesis; D005312:Fetal Blood / Q000032:analysis / Q000502:physiology*; D006098:Granulocytes / Q000166:cytology; D006801:Humans; D007202:Indicators and Reagents; D008264:Macrophages / Q000166:cytology; D008970:Molecular Weight; D011506:Proteins / Q000302:isolation & purification / Q000502:physiology
D007202:Indicators and Reagents; D011506:Proteins; D002606:Charcoal; C016313:colony inhibiting factor