The effects of p-mercuribenzenesulfonate on purified spectrin and actin.
The compound p-mercuribenzenefulfonate was found to affect the self-association behavior of both spectrin and actin. The reagent brings about the depolymerization of F-actin, as judged from the decrease in the fluorescence of an attached pyrene label, with a second-order rate constant an order of magnitude less than that for the disruption of isolated erythrocyte cytoskeletons. Therefore, it is unlikely that the depolymerization of actin is the rate-determining step in the mercurial-dependent disruption of the erythrocyte cytoskeleton. Low reagent concentrations caused an initial rapid dissociation of spectrin tetramers at a rate comparable with that of cytoskeleton disruption. Prolonged incubation, or higher reagent concentrations, resulted in subsequent aggregation of spectrin. The reagent also prevented the interaction between spectrin and actin, presumably through its depolymerization of actin and its effects on spectrin. The early event in the disruption of isolated erythrocyte cytoskeletons by p-mercuribenzenesulfonate thus appears to be the dissociation of spectrin oligomers. Subsequent depolymerization of actin brought about by the reagent then results in total disruption of the cytoskeleton.
Clark
S J
SJ
Department of Biochemistry, University of Sydney, Australia.
O'Brien
M E
ME
Ralston
G B
GB
D000199:Actins / Q000097:blood; D004591:Electrophoresis, Polyacrylamide Gel; D004910:Erythrocyte Membrane / Q000187:drug effects* / Q000648:ultrastructure; D005033:Ethylmaleimide / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D046911:Macromolecular Substances; D008626:Mercuribenzoates / Q000494:pharmacology*; D011108:Polymers; D011485:Protein Binding / Q000187:drug effects; D013049:Spectrin / Q000187:drug effects*
D000199:Actins; D046911:Macromolecular Substances; D008626:Mercuribenzoates; D011108:Polymers; D013049:Spectrin; D005033:Ethylmaleimide