Intracellular metabolism of the interferon mediator, 2-5A, using permeabilized cells.
2-5A synthetase and 2'-phosphodiesterase, the enzymatic activities which respectively synthesize and degrade the interferon mediator 2-5A (ppp(A2'p)nA), were studied in digitonin-permeabilized cells. 2-5A synthetase was higher in permeabilized than in lysed Daudi cells. Mouse L cells appeared to contain two different 2-5A synthetase activities, one of which could be separated from 2'-phosphodiesterase activity, which was only cytosolic. Permeabilization techniques offer opportunities to investigate (2',5')-oligoadenylate intracellular metabolism, which remains incompletely known.
Luxembourg
A
A
Centre d'Immunologie INSERM-CNRS de Marseille-Luminy, France.
D015088:2',5'-Oligoadenylate Synthetase / Q000378:metabolism; D000227:Adenine Nucleotides / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000201:enzymology; D002463:Cell Membrane Permeability / Q000187:drug effects; D004072:Digitonin / Q000494:pharmacology; D005095:Exoribonucleases / Q000378:metabolism; D006801:Humans; D007739:L Cells / Q000378:metabolism; D051379:Mice; D009843:Oligoribonucleotides / Q000378:metabolism*; D011500:Protein Synthesis Inhibitors
D000227:Adenine Nucleotides; D009843:Oligoribonucleotides; D011500:Protein Synthesis Inhibitors; C023505:2',5'-oligoadenylate; D015088:2',5'-Oligoadenylate Synthetase; D005095:Exoribonucleases; C029364:2'-phosphodiesterase; D004072:Digitonin