Reduced amounts of S-adenosylmethionine decarboxylase in the adrenal glands of rats following administration of piribedil or 2-deoxyglucose.
The activity of S-adenosylmethionine decarboxylase (SAM-DC) is decreased in the adrenal gland of the rat following physical stress, metabolic stress or administration of dopamine agonists [M. Ekker and T. L. Sourkes, Endocrinology 120, 1299 (1987)]. Immunotitration studies with a serum directed against purified rat liver SAM-DC show that the reduction in activity of the enzyme following administration of 2-deoxyglucose or piribedil was paralleled by a decrease in the amount of immuno-reactive protein. There was no difference in the half-life of SAM-DC activity between piribedil-treated rats and controls. The properties of an extensively purified preparation of the adrenal enzyme resembled those of SAM-DC obtained from rat liver. It is suggested that the reduction in adrenal SAM-DC activity and protein content caused by stress is due to a reduction in the rate of synthesis of the enzyme.
Ekker
M
M
Department of Biochemistry, McGill University, Faculty of Medicine, Montreal, Quebec, Canada.
Sourkes
T L
TL
Gabor
R
R
D012437:Adenosylmethionine Decarboxylase / Q000032:analysis*; D000311:Adrenal Glands / Q000201:enzymology*; D000818:Animals; D002262:Carboxy-Lyases / Q000032:analysis*; D003513:Cycloheximide / Q000494:pharmacology; D003837:Deoxy Sugars / Q000494:pharmacology*; D003847:Deoxyglucose / Q000494:pharmacology*; D008297:Male; D008970:Molecular Weight; D009955:Ornithine Decarboxylase / Q000032:analysis; D010879:Piperazines / Q000494:pharmacology*; D010891:Piribedil / Q000494:pharmacology*; D011700:Putrescine / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains
D003837:Deoxy Sugars; D010879:Piperazines; D003513:Cycloheximide; D003847:Deoxyglucose; D010891:Piribedil; D002262:Carboxy-Lyases; D009955:Ornithine Decarboxylase; D012437:Adenosylmethionine Decarboxylase; D011700:Putrescine