[Isolation and analysis of Ca/Mg-dependent endonuclease from cell nuclei of human splenocytes].
A scheme for the isolation of Ca,Mg-dependent endonuclease from human spleen lymphocyte nuclei has been developed. The isolation procedure resulted in protein preparations (Mr = 57 kD) possessing an enzymatic activity and stable upon storage for over a period of one year. The enzyme is an endonuclease which predominantly cleaves double-stranded DNA by a mixed single- and double-hit mechanism with the formation of 5'-phosphate and 3'-OH terminal groups. Its maximal activation is induced by Ca2+ plus Mg2+. The enzyme is also active in the presence of Mn2+, Ca2+, Mg2+ and Zn2+ and is inhibited by Co2+. NaCl and KCl (0.15-0.2 M) and p-chloromercuribenzoate (1 mM) also inhibit the enzyme. ATP has no activating effect.
Sokolova
I A
IA
Khodarev
N N
NN
Aleksandrova
S S
SS
Votrin
I I
II
D002467:Cell Nucleus / Q000201:enzymology; D004277:DNA, Single-Stranded / Q000378:metabolism; D004706:Endodeoxyribonucleases / Q000302:isolation & purification* / Q000378:metabolism; D004789:Enzyme Activation; D006801:Humans; D008214:Lymphocytes / Q000201:enzymology*; D008970:Molecular Weight; D013154:Spleen / Q000166:cytology / Q000201:enzymology*; D013379:Substrate Specificity
D004277:DNA, Single-Stranded; D004706:Endodeoxyribonucleases; C029367:calcium magnesium dependent endodeoxyribonuclease
Vydelenie i issledovanie Ca/Mg-zavisimoĭ éndonukleazy iz kletochnykh iader limfotsitov selezenki cheloveka.