Genotoxic effects of MeCCNU on human peripheral blood lymphocytes.
MeCCNU (semustine, 1-(2-chloroethyl)-3-(4-methylcyclohexyl)-1-nitrosourea, NSC 95441) is successfully used in the treatment of various human malignancies. The drug was tested for its in vitro genotoxic effects on human peripheral blood lymphocytes. Several drug concentrations were studied considering mean plasma level achieved in patients after the receipt of MeCCNU therapy. Induction of chromosome aberrations and sister chromatid exchange frequency had shown dose-effect relationship. Reduced mitotic activity and prolonged average generation time was observed in MeCCNU treated cultures. The results suggest that 'therapeutic' MeCCNU concentrations have genotoxic effects on human peripheral blood lymphocytes.
Vyas
R C
RC
Cell Biology Division, Gujarat Cancer Research Institute, Ahmedabad, India.
Adhvaryu
S G
SG
Shah
V C
VC
D000284:Administration, Oral; D000328:Adult; D002869:Chromosome Aberrations / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000187:drug effects*; D008940:Mitotic Index; D009153:Mutagens; D012673:Semustine / Q000097:blood / Q000633:toxicity*; D012854:Sister Chromatid Exchange / Q000187:drug effects
D009153:Mutagens; D012673:Semustine