[The value of measuring membrane potentials of urothelial cells using cytofluorometry in the study of tumors of the bladder. Preliminary analysis].
In the context of investigation of a prognostic marker applicable to bladder tumours, the authors propose the cytofluorometric study of the membrane potential of malignant urothelial cells using a molecular probe, 3,3' dihexyloxacarbocyanine. This preliminary study demonstrated a significant increase in the membrane potential of malignant urothelial cells in comparison with normal cells, which leads the authors to propose this cellular parameter as a new tool in the prognostic evaluation of bladder tumours.
Nicolas
J
J
Centre de Recherches du Service de Santé des Armées, Clamart.
Vieillefond
A
A
Benoit
G
G
Jardin
A
A
Neveux
Y
Y
Martin
E
E
D014408:Biomarkers, Tumor / Q000302:isolation & purification; D002232:Carbocyanines; D004121:Dimethyl Sulfoxide; D004848:Epithelium / Q000473:pathology / Q000503:physiopathology; D005434:Flow Cytometry; D005456:Fluorescent Dyes; D006801:Humans; D008564:Membrane Potentials; D015335:Molecular Probes; D011379:Prognosis; D001749:Urinary Bladder Neoplasms / Q000473:pathology* / Q000503:physiopathology
D014408:Biomarkers, Tumor; D002232:Carbocyanines; D005456:Fluorescent Dyes; D015335:Molecular Probes; C007392:3,3'-dihexyl-2,2'-oxacarbocyanine; D004121:Dimethyl Sulfoxide
Intérêt de la mesure du potentiel de membrane des cellules urothéliales en cytofluorométrie dans l'étude des tumeurs de vessie. Analyses préliminaires.