title: A model system for the in vivo determination of the thrombolytic effect of plasminogen activators.
abstract: An in vivo model system for measuring the thrombolytic efficiency of plasminogen activators was used. The formation of radiolabelled microthrombi was induced by infusion with I-125 labelled fibrinogen and thrombin. Reactive fibrinolysis was inhibited by administration of suboptimal levels of e-aminocaproic acid. The thrombolytic and subsequent fibrinolytic events were followed in the capillary bed of the lungs of anesthetized rats by external monitoring of the I-125 activity over the lung field. The model was successfully employed to demonstrate the thrombolytic effect of plasminogen activator produced by a transplanted spontaneous rat prostate adenocarcinoma cell line (PA III). The system proved to be reproducible with detection limits of 6000 I.U. using the PA-III cell line derived activator.
authors.affiliation: Lobund Laboratory, University of Notre Dame, Indiana 46556.
authors.forename: J D
authors.initials: JD
authors.lastname: Scheu
authors.forename: S L
authors.initials: SL
authors.lastname: Jing
authors.forename: E
authors.initials: E
authors.lastname: Bruckner-Kardoss
authors.forename: P E
authors.initials: PE
authors.lastname: Gargan
authors.forename: M
authors.initials: M
authors.lastname: Pollard
mesh_terms: D000818:Animals; D005260:Female; D005340:Fibrinogen / Q000378:metabolism / Q000494:pharmacology; D005342:Fibrinolysis / Q000187:drug effects; D005343:Fibrinolytic Agents; D008171:Lung Diseases / Q000209:etiology; D008297:Male; D010960:Plasminogen Activators / Q000378:metabolism / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D013917:Thrombin / Q000494:pharmacology; D013927:Thrombosis / Q000209:etiology; D014407:Tumor Cells, Cultured / Q000378:metabolism
chemical_list: D005343:Fibrinolytic Agents; D005340:Fibrinogen; D010960:Plasminogen Activators; D013917:Thrombin