title: Chemical synthesis of DNA oligomers containing cytosine arabinoside.
abstract: The solid phase phospite triester synthesis of oligodeoxynucleotides containing cytosine arabinoside (araC) is described. A protected araC phosphoramadite was prepared for the introduction of araC residues at 5'termini and internucleotide positions in DNA oligomers. These oligomers were utilized to demonstrate the formation of correct 3'-5' linkages, to test for alkaline lability at the araC site, and to study the stability of duplexes containing araC-G base pairs. For the introduction of araC residues at 3' terminal positions, a protected derivative of araC was coupled to functionalized silica. This material was used to prepare a test oligomer which was characterized enzymatically.
authors.affiliation: Department of Pediatrics, Yale University School of Medicine, New Haven, CT 06510.
authors.forename: G P
authors.initials: GP
authors.lastname: Beardsley
authors.forename: T
authors.initials: T
authors.lastname: Mikita
authors.forename: M M
authors.initials: MM
authors.lastname: Klaus
authors.forename: A L
authors.initials: AL
authors.lastname: Nussbaum
mesh_terms: D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D003561:Cytarabine; D009691:Nucleic Acid Denaturation; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis*; D013696:Temperature
chemical_list: D009838:Oligodeoxyribonucleotides; D003561:Cytarabine