title: Interaction of vanadyl ribonucleoside complex with the androgen receptor.
abstract: The addition of vanadyl ribonucleoside complex (VRC), a potent inhibitor of RNase, to the transformed 4.5S androgen receptor from rat submandibular gland caused an increase in the sedimentation coefficient to 7.0S. Moreover, VRC decreased the DNA-cellulose binding of the transformed receptor; 50% inhibition of the DNA-cellulose binding was achieved at 1.8 mM VRC. On the other hand, agents related to VRC and oxoanions of transient metals, such as ribonucleoside, vanadate, molybdate, tungstate and arsenate, exerted no effect on the DNA-cellulose binding ability of the receptor. These findings suggest that VRC binds to the transformed androgen receptor at the DNA-binding site and that both oxovanadium ion and ribonucleoside are indispensable for the binding of VRC to the transformed androgen receptor.
authors.affiliation: Department of Biochemistry, Iwate Medical University School of Dentistry, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Ohara-Nemoto
authors.forename: M
authors.initials: M
authors.lastname: Yoshida
authors.forename: M
authors.initials: M
authors.lastname: Ota
mesh_terms: D000818:Animals; D001665:Binding Sites; D002482:Cellulose / Q000031:analogs & derivatives / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D004247:DNA / Q000031:analogs & derivatives / Q000378:metabolism; D005260:Female; D066298:In Vitro Techniques; D051381:Rats; D011919:Rats, Inbred Strains; D011944:Receptors, Androgen / Q000187:drug effects / Q000378:metabolism*; D012259:Ribonuclease, Pancreatic / Q000494:pharmacology; D012263:Ribonucleosides / Q000378:metabolism*; D013363:Submandibular Gland / Q000378:metabolism; D014638:Vanadates / Q000378:metabolism*
chemical_list: C033190:DNA-cellulose; D011944:Receptors, Androgen; D012263:Ribonucleosides; C057724:vanadyl ribonucleoside complex; D014638:Vanadates; D002482:Cellulose; D004247:DNA; D012259:Ribonuclease, Pancreatic