Molecular cloning of bovine beta-lactoglobulin cDNA.
A cDNA library from bovine mammary gland mRNA was constructed in pBR322 and screened by hybrid-selected translation and immunoscreening. Several beta-lactoglobulin clones were identified and sequenced. All clones contained cDNA fragments corresponding to the 3' region of beta-lactoglobulin mRNA. The 3' non-translated region of beta-lactoglobulin mRNA consists of 187 nucleotides; the polyadenylation signal AATAAA occurs 17 nucleotides before the poly(A) tail. The amino-acid sequence predicted from the 3' coding region corresponds completely to the previously determined amino-acid sequence of beta-lactoglobulin.
Ivanov
V N
VN
N.I. Vavilov Institute of General Genetics, USSR Academy of Sciences, Moscow.
Judinkova
E S
ES
Gorodetsky
S I
SI
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D005260:Female; D005796:Genes; D007782:Lactoglobulins / Q000235:genetics*; D008321:Mammary Glands, Animal / Q000378:metabolism; D008969:Molecular Sequence Data; D012689:Sequence Homology, Nucleic Acid; D012756:Sheep; D013045:Species Specificity
D007782:Lactoglobulins; D004247:DNA