title: Strategy for molecular cloning of the fragile X site DNA.
abstract: Fragile X syndrome is a common form of mental retardation associated with a fragile site on the human X chromosome. We have recently demonstrated that the fragile X chromosome, when isolated within a somatic cell hybrid, often participates in translocations involving rodent chromosome arms. Cytogenetic and molecular evidence strongly suggests that the human breakpoint of these translocations is within the fragile X sequence. Hence, the joining of heterologous DNA (i.e. from two species) may permit the molecular cloning of the fragile X site. We describe here the cloning approach employed to enhance the isolation of interspecific chromosome translocation junctions. The human portion of the translocation junction should be derived from the fragile X site sequence.
authors.affiliation: Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia 30322.
authors.forename: S T
authors.initials: ST
authors.lastname: Warren
authors.forename: F P
authors.initials: FP
authors.lastname: Zhang
authors.forename: J S
authors.initials: JS
authors.lastname: Sutcliffe
authors.forename: J F
authors.initials: JF
authors.lastname: Peters
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D003360:Cosmids; D004274:DNA, Recombinant; D005600:Fragile X Syndrome / Q000235:genetics*; D006801:Humans; D006822:Hybrid Cells; D012729:Sex Chromosome Aberrations / Q000235:genetics*; D014178:Translocation, Genetic; D014960:X Chromosome
chemical_list: D004274:DNA, Recombinant