[Gamma-cystathionase: the non-ideal gel filtration high performance liquid chromatography. Physico-chemical and immunochemical characteristics of the enzyme].
The rapid method for gamma-cystathionase purification was developed. It is based on the non-ideal gel filtration HPLC. The isolated homogeneous enzyme was used for immunization and immunosorbent preparation. A monospecific polyclonal antibody was prepared. The substrate specificity of the isolated enzyme was studied.
Gabikov
A G
AG
Shuster
A M
AM
Kazakov
V K
VK
Kondrashina
N N
NN
Goriachenkova
E V
EV
D000911:Antibodies, Monoclonal; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003542:Cystathionine gamma-Lyase / Q000276:immunology / Q000302:isolation & purification*; D004247:DNA / Q000235:genetics; D015151:Immunoblotting; D008190:Lyases / Q000302:isolation & purification*; D013379:Substrate Specificity
D000911:Antibodies, Monoclonal; D004247:DNA; D008190:Lyases; D003542:Cystathionine gamma-Lyase
Gamma-tsistationaza: vysokoéffektivnaia zhidkostnaia khromatografiia v rezhime neideal'noĭ gel'-fil'tratsii. Fiziko-khimicheskaia i immunokhimicheskaia kharakteristika fermenta.