title: Effects of ethanol on in vitro ciliary motility.
abstract: Consumption of ethanol can impair lung function and slow total lung clearance. High concentrations of ethanol have been shown to slow or arrest ciliary beating. This study examined the effects of concentrations of alcohol comparable to blood levels achieved from social drinking on ciliary beat frequency. We obtained ciliated cells by brushing the trachea of unanesthetized sheep during fiber-optic bronchoscopy. The cells were suspended in a perfusion chamber and physiological conditions were maintained in vitro. Ciliary beat frequency and synchrony were determined by slow-motion analysis of video images obtained by interference contrast microscopy. Metachronal ciliary coordination was observed in all preparations. The ciliary beat frequency was stimulated at ethanol concentrations from 0.01 up to but not including 0.1%, unchanged at 0.5 and 1%, and slowed at 2%. While confirming inhibition of ciliary motility at very high ethanol levels, we observed no acute impairment of ciliary function at ethanol concentrations comparable to those achieved from social drinking. Indeed, we found an unexpected stimulation of ciliary beating at low levels of ethanol. How this alteration in ciliary beating would affect pulmonary clearance remains unknown at this time.
authors.affiliation: Division of Pulmonary Disease, Mount Sinai Medical Center, Miami Beach, Florida 33140.
authors.forename: D R
authors.initials: DR
authors.lastname: Maurer
authors.forename: J
authors.initials: J
authors.lastname: Liebman
mesh_terms: D000818:Animals; D002923:Cilia / Q000187:drug effects / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000494:pharmacology*; D066298:In Vitro Techniques; D009068:Movement; D009079:Mucociliary Clearance / Q000187:drug effects; D012756:Sheep; D014132:Trachea / Q000648:ultrastructure*
chemical_list: D000431:Ethanol