Sensitive assay of cysteine proteinases using new peptide p-nitroanilides.
N-Succinyl-glycyl-leucyl-cystein(S-benzyl) p-nitroanilide and N-succinyl-leucyl-leucyl-cystein(S-benzyl) p-nitroanilide were found to be very sensitive substrates for the assay of papain, ficin, and bromelain. These p-nitroanilides were hydrolyzed only very slightly by chymotrypsin, but not detectably by trypsin.
Fukal
L
L
Kasafírek
E
E
D001963:Bromelains / Q000032:analysis; D003546:Cysteine Endopeptidases / Q000032:analysis*; D005361:Ficain / Q000032:analysis; D006868:Hydrolysis; D009842:Oligopeptides; D010206:Papain / Q000032:analysis
D009842:Oligopeptides; D001963:Bromelains; D003546:Cysteine Endopeptidases; D010206:Papain; D005361:Ficain