Optical recording with single cell resolution from monolayered slice cultures of rat hippocampus.
Voltage-sensitive dyes were used to optically record the membrane potentials from neurons in hippocampal slice cultures. Multi-channel recordings from these monolayered but otherwise 'organotypic' slice cultures had very good spatial as well as good temporal resolution (15 x 15 micron, 0.5 ms respectively). We show that in this preparation action potentials elicited by intracellular current pulses can be recorded optically and that single spikes are readily detectable without averaging. Furthermore, a new procedure which significantly reduces photodynamic damage is described. Our study demonstrates the feasibility of optical recording with single cell resolution in an organotypic mammalian CNS preparation.
Bonhoeffer
T
T
Max-Planck-Institut für biologische Kybernetik, Tübingen, F.R.G.
Staiger
V
V
D000818:Animals; D004558:Electric Stimulation; D005456:Fluorescent Dyes; D006624:Hippocampus / Q000502:physiology*; D066298:In Vitro Techniques; D008564:Membrane Potentials; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*; D011726:Pyridinium Compounds; D051381:Rats
D005456:Fluorescent Dyes; D011726:Pyridinium Compounds; C048153:4-(6-(4-dibutylaminophenyl)-1,3,5-hexatrienyl)-1-(4'-sulfobutyl)pyridinium; C054158:RH 414