Effect of fagaronine on cell cycle progression of human erythroleukemia K562 cells.
Fagaronine (Fine) is a novel antileukemic drug extracted from Fagara xanthoxyloides Lam. (Rutaceae). In an attempt to know more about its mechanism of action we describe here its inhibitory activity on cell division, 3H-thymidine incorporation and on cell cycle progression. Fine inhibits cell proliferation of K562 cells by 50% at a concentration of 3 x 10(-6) mol/l at day 4. It stimulates incorporation of labelled macromolecular thymidine on day 1, but decreases incorporation on days 2, 3 and 4. Fine induces a cell accumulation in G2 and late-S phases. This accumulation (i) increases with Fine concentration, but a complete blockade is not observed, (ii) reaches a plateau after approximately 48 h, (iii) is reversible, whereas we have previously shown that cell growth inhibition and differentiation were not reversible.
Comoë
L
L
Faculté de Pharmacie, Abidjan, Côte d'Ivoire.
Carpentier
Y
Y
Desoize
B
B
Jardillier
J C
JC
D000470:Alkaloids / Q000633:toxicity*; D000972:Antineoplastic Agents, Phytogenic / Q000633:toxicity*; D053119:Benzophenanthridines; D002453:Cell Cycle / Q000187:drug effects*; D002460:Cell Line; D004247:DNA / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D006131:Growth Inhibitors / Q000633:toxicity; D006801:Humans; D004915:Leukemia, Erythroblastic, Acute / Q000378:metabolism / Q000473:pathology*; D008940:Mitotic Index / Q000187:drug effects; D010617:Phenanthridines; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors
D000470:Alkaloids; D000972:Antineoplastic Agents, Phytogenic; D053119:Benzophenanthridines; D006131:Growth Inhibitors; D010617:Phenanthridines; C002066:fagaronine; D004247:DNA; D013936:Thymidine