Presence of the Caenorhabditis elegans spliced leader on different mRNAs and in different genera of nematodes.
Several different mRNAs from Caenorhabditis elegans contain the same 22-nucleotide leader sequence at their 5' ends that is acquired in a trans-splicing reaction. About 10 to 15% of the major proteins are translated from mRNAs that contain the spliced leader, among them two ribosomal proteins, ubiquitin, GAPDH, a heat shock protein (hsp70a), and three actins. The same spliced leader sequence is present in mRNAs isolated from nematodes from several different genera; but it is not present in mRNAs from other organisms. The spliced leader is encoded as a spliced leader (SL) RNA about 100 nucleotides long. The gene for the SL RNA is located in the 5S rDNA repeat in C. elegans; however, this association with the 5S repeat is not preserved in other genera. The 22-nucleotide spliced leader sequence is conserved in three genera of nematodes.
Bektesh
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Synergen Inc., Boulder, Colorado 80301.
Van Doren
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Hirsh
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D000199:Actins / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D002107:Caenorhabditis / Q000235:genetics*; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D009348:Nematoda / Q000235:genetics*; D015345:Oligonucleotide Probes; D015347:RNA Probes; D012326:RNA Splicing; D012333:RNA, Messenger; D013045:Species Specificity
D000199:Actins; D015345:Oligonucleotide Probes; D015347:RNA Probes; D012333:RNA, Messenger