Production and characterization of a monoclonal antibody to the trichothecene mycotoxin diacetoxyscirpenol.
A monoclonal antibody was obtained by the fusion of mouse myeloma cells with splenocytes isolated from Balb/c mice, which had been immunized with diacetoxyscirpenol-hemiglutarate (DAS-hemiglutarate) and verrucarol-hemiglutarates covalently bound to ethylenediamine-modified bovine serum albumin. The anti-DAS-antibody that could be induced was of the IgM type with kappa-chains. The titer of the monoclonal anti-DAS-antibody in ascites fluid obtained from mice injected the selected cell line was much higher than those of conventional antisera. An enzyme-linked immunosorbent assay based on the competitive binding principle in which the antibody was applied had a sensitivity of 1 ng DAS per assay. The relative cross-reactivity of the monoclonal antibody in the CI-ELISA with the related trichothecenes such as triacetoxyscirpenol, 15-monoacetoxyscirpenol, diacetylverrucarol, 4-monoacetoxyscirpenol and scirpentriol were found to be 1.8, 0.8, 0.15, 0.02 and less than 0.001, respectively. The trichothecenes verrucarol, T-2 toxin, T-2 tetraol, deoxynivalenol, 3-acetyldeoxynivalenol and trichothecin showed no cross-reactivity.
Pauly
J U
JU
Institut für Medizinische Mikrobiologie, Universität Mainz.
Bitter-Suermann
D
D
Dose
K
K
D000818:Animals; D000911:Antibodies, Monoclonal; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D009183:Mycotoxins / Q000276:immunology*; D012717:Sesquiterpenes / Q000276:immunology*; D014255:Trichothecenes / Q000032:analysis / Q000276:immunology*
D000911:Antibodies, Monoclonal; D009183:Mycotoxins; D012717:Sesquiterpenes; D014255:Trichothecenes; C001809:diacetoxyscirpenol