Teat skin normal flora and colonization with mastitis pathogen inhibitors.
Isolates of bacteria from normal teats were used to attempt colonization of teats of dry cows or neonatal calves. Isolates for inoculation were chosen on the basis of ability to inhibit mastitis pathogens in vitro, with the ultimate goal of in vivo inhibition of mastitis pathogens at the teat surface. Three bacterial normal flora isolates (Corynebacterium xerosis, Bacillus sp. and Aerococcus viridans) persisted less than 10 days on the teats of dry cows. The fourth isolate, Staphylococcus hominis 1, was studied in greatest detail because studies characterizing the normal teat flora showed staphylococci to be the predominant flora. The S. hominis 1 isolated used for inoculation was an inhibitor of Gram-positive mastitis pathogens. It was a biotype not found on these teats prior to inoculation, thus facilitating identification of the inoculated isolate on sequential sampling. Colonization of newborn calves, before other bacterial flora became established, resulted in recovery of inoculated S. hominis 1 for an average of 51 days or longer. On dry cow teats it was detected for up to 28 days. On several occasions the inoculated S. hominis 1 was found in pure culture. Since many new infections occur during the dry period, the colonization of dry cow teats with S. hominis 1 organisms inhibitory for Gram-positive pathogens should be tested as an adjunct to other methods of mastitis prevention.
Woodward
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WD
College of Veterinary Medicine, Washington State University, Pullman 99164-7040.
Ward
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AC
Fox
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LK
Corbeil
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LB
D000818:Animals; D000831:Animals, Newborn; D001407:Bacillus / Q000254:growth & development; D001419:Bacteria / Q000254:growth & development*; D002417:Cattle; D003352:Corynebacterium / Q000254:growth & development; D005260:Female; D008321:Mammary Glands, Animal / Q000382:microbiology*; D008414:Mastitis, Bovine / Q000276:immunology / Q000382:microbiology*; D013210:Staphylococcus / Q000254:growth & development; D013289:Streptococcaceae / Q000254:growth & development