title: Calcium regulates the regeneration of cilia in Tetrahymena thermophila.
abstract: A study of calcium metabolism in Tetrahymena during the regeneration of cilia evidenced that the process is inhibited by nifedipine and trifluoperazine. This suggests that calcium ions play an important regulatory role in this process. This was confirmed by studies on calcium uptake and efflux which showed that there was a net increase in calcium uptake prior to the reinitiation of motility. The increase coincided with a period of sensitivity to the calcium antagonist TMB-8 and with an increase in the intracellular level of cGMP. The process was also inhibited by neomycin and stimulated by phorbol esters, which suggests that hydrolysis of phosphatidylinositol phosphates may take place as part of the calcium regulatory network during the regeneration of cilia.
authors.affiliation: Biology Department, University of Puerto Rico, Rio Piedras 00931.
authors.forename: J A
authors.initials: JA
authors.lastname: Joglar-Ramirez
authors.forename: F L
authors.initials: FL
authors.lastname: Renaud
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002121:Calcium Channel Blockers / Q000494:pharmacology; D002465:Cell Movement; D002923:Cilia / Q000187:drug effects / Q000502:physiology; D003995:Dibutyryl Cyclic GMP / Q000494:pharmacology; D005707:Gallic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D009355:Neomycin / Q000494:pharmacology; D009543:Nifedipine / Q000494:pharmacology; D010703:Phorbol Esters / Q000494:pharmacology; D012038:Regeneration; D013768:Tetrahymena / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D014268:Trifluoperazine / Q000494:pharmacology
chemical_list: D002121:Calcium Channel Blockers; D010703:Phorbol Esters; D014268:Trifluoperazine; D003995:Dibutyryl Cyclic GMP; C006014:8-(N,N-diethylamino)octyl-3,4,5-trimethoxybenzoate; D005707:Gallic Acid; D009355:Neomycin; D009543:Nifedipine; D002118:Calcium