[Experimental study on therapeutic plasmapheresis with the biliary decompression in the treatment of obstructive jaundice].
The effectiveness of therapeutic plasmapheresis with the biliary decompression was evaluated using animal model in the treatment of obstructive jaundice. Plasma exchange (PE) using fresh frozen plasma was carried out with biliary decompression on canine jaundice model made by means of ligation and resection of bile duct. Routine biochemical analysis was performed following the PE and biliary drainage and the result was compared with the external biliary drainage group. Plasma level of total bilirubin decreased after PE and kept lower level while plasma level of bilirubin in external biliary drainage group decreased slowly. M-GOT and GOT level, those are the indicators of liver cell injury, was lower in PE group. Mitochondrial function of liver cell was evaluated following partial hepatectomy carried out two days after PE with biliary decompression on jaundiced rat. Mitochondrial respiratory control ratio and ADP/O ratio was improved in PE group. The effectiveness of PE is speculated as the combined mechanism of the removal of hepatotoxic factors in the jaundiced plasma and the addition of hepatotrophic factors within the fresh frozen plasma. These results support the effectiveness of PE to shorten the biliary drainage period and to improve the hepatic function for perioperative management.
Kimata
H
H
First Department of Surgery, Kobe University, School of Medicine, Japan.
Ohyanagi
H
H
Usami
M
M
Saitoh
Y
Y
D000818:Animals; D001663:Bilirubin / Q000097:blood; D002779:Cholestasis / Q000503:physiopathology / Q000601:surgery / Q000628:therapy*; D003131:Combined Modality Therapy; D004285:Dogs; D008099:Liver / Q000503:physiopathology; D008111:Liver Function Tests; D008297:Male; D008930:Mitochondria, Liver / Q000378:metabolism / Q000503:physiopathology; D010956:Plasmapheresis / Q000379:methods; D051381:Rats; D011919:Rats, Inbred Strains
D001663:Bilirubin