title: Flow cytometric analysis of platelet surface antigens.
abstract: A flow cytometric analysis of human platelet surface antigens was carried out using a panel of monoclonal antibody reagents. The reagents used were specific either for the GPIb or the GP IIb/IIIa complex, surface immunoglobulin, or von Willebrand factor (vWf). Indirect surface immunophenotypes were determined using an EPICS V flow cytometer and the monoclonal antibodies 6D1, 10E5, Plt-1, UR1663, anti-IgG, and anti-vWf. Platelets were obtained from normal individuals or patients with either Bernard-Soulier syndrome (BSS) or Glanzmann's thrombasthenia (GT). Normal platelets were positive for 10E5, 6D1, Plt-1, and UR1663 and showed negligible activity for anti-IgG and anti-vWf. Platelets from individuals with BSS showed a marked reduction in 6D1, while the platelets of a patient with GT showed a marked reduction in binding of 10E5, Plt-1, and UR1663. Differences between histograms for normal platelets and for platelets from individuals with BSS or GT were evaluated using the Kolmogorov-Smirnov test. Compared to normal platelets, the BSS and GT platelets contain at least 35-fold less of the GPIb and GP IIb/IIIa complex respectively. Flow cytometry is a useful and precise method for the study of normal and abnormal surface platelet phenotypes.
authors.affiliation: Hematology Service, National Institutes of Health, Bethesda, Maryland 20892.
authors.forename: G E
authors.initials: GE
authors.lastname: Marti
authors.forename: L
authors.initials: L
authors.lastname: Magruder
authors.forename: W E
authors.initials: WE
authors.lastname: Schuette
authors.forename: H R
authors.initials: HR
authors.lastname: Gralnick
mesh_terms: D000911:Antibodies, Monoclonal; D001606:Bernard-Soulier Syndrome / Q000276:immunology; D001792:Blood Platelets / Q000276:immunology* / Q000473:pathology; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D010980:Platelet Membrane Glycoproteins / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D010980:Platelet Membrane Glycoproteins