title: Xanthine and uric acid levels in rat brain following focal ischemia.
abstract: Changes of the xanthine and uric acid (UA) levels in rat forebrain following focal cerebral ischemia were studied by reversed-phase HPLC with electrochemical detection. Focal ischemia was induced by occluding the left middle cerebral artery in the rat. The xanthine level in the normal group was 11.50 nmol/g tissue. In the ischemic group, the xanthine concentration in the ischemic hemisphere progressively increased after occlusion and reached a maximum value of 59.42 nmol/g tissue 4 h after operation. The UA level in the normal group was 2.20 nmol/g tissue, whereas in the ischemic group the UA concentration in the ischemic hemisphere gradually increased after occlusion, reaching a value of 38.53 nmol/g tissue 24 h after ischemia. The concentration of UA remained elevated in the ischemic hemisphere until 48 h after occlusion, and reached a maximum value of 38.98 nmol/g tissue. The xanthine and UA levels in the contralateral hemisphere remained unchanged. The xanthine and UA concentrations in the sham-operated group did not show a significant increase after operation. The time course of xanthine and UA levels suggests that in ischemic forebrain UA is formed from xanthine as a product of purine metabolism.
authors.affiliation: Department of Neurosurgery, Teikyo University School of Medicine, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Kanemitsu
authors.forename: A
authors.initials: A
authors.lastname: Tamura
authors.forename: T
authors.initials: T
authors.lastname: Kirino
authors.forename: S
authors.initials: S
authors.lastname: Karasawa
authors.forename: K
authors.initials: K
authors.lastname: Sano
authors.forename: T
authors.initials: T
authors.lastname: Iwamoto
authors.forename: M
authors.initials: M
authors.lastname: Yoshiura
authors.forename: K
authors.initials: K
authors.lastname: Iriyama
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D002546:Ischemic Attack, Transient / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D014527:Uric Acid / Q000097:blood / Q000378:metabolism*; D019820:Xanthine; D014969:Xanthine Oxidase / Q000494:pharmacology; D014970:Xanthines / Q000378:metabolism*
chemical_list: D014970:Xanthines; D019820:Xanthine; D014527:Uric Acid; D014969:Xanthine Oxidase