Trophoblast antigens in human seminal plasma.
Secondary recurrent spontaneous (2 degrees) aborters manifest persistent IgG, which show differential cytotoxicity patterns with lymphocytes from many donors. These are non-HLA-directed antibodies, which react allotypically with both trophoblast and lymphocytes. The antigens they recognize are designated trophoblast-lymphocyte crossreactive (TLX) antigens. Xenogeneic anti-TLX sera were studied with the use of enzyme-linked-immunosorbent (ELISA) and immunochemical assays to determine the TLX status of seminal plasma. The results showed 1) allotypic TLX antigens are present in seminal plasma; 2) seminal plasma TLX antigens may be membrane associated; 3) by immunoblotting, the molecular weights of antigens reactive with TLX antisera are 15, 22, 28, 33 kD and a smear between 180 and 340 kd; 4) by isoelectric focusing, TLX antigens show pI 4.0, 5.35, 5.9, 6.5, 6.8, and 7.2. Allotypic seminal plasma TLX antigens may provide the antigenic stimuli for persistent maternal humoral immunity.
Kajino
T
T
Center for Reproduction and Transplantation Immunology, Methodist Hospital of Indiana, Indianapolis 46202.
Torry
D S
DS
McIntyre
J A
JA
Faulk
W P
WP
D000026:Abortion, Habitual / Q000276:immunology; D001324:Autoantigens / Q000302:isolation & purification*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D015151:Immunoblotting; D007518:Isoantibodies / Q000096:biosynthesis; D007519:Isoantigens; D008297:Male; D011247:Pregnancy; D012661:Semen / Q000276:immunology*; D014327:Trophoblasts / Q000276:immunology*
D001324:Autoantigens; D007518:Isoantibodies; D007519:Isoantigens; C055067:trophoblast-lymphocyte cross-reactive antigens