Development of a radioimmunoassay for isometamidium.
A radioimmunoassay for the trypanocidal drug isometamidium (ISMM) has been developed using antibodies produced in sheep immunized against the drug cross-linked to human serum albumin. With 14C-ISMM as a radioligand, cross-reactivity of the antibodies was determined and found to be less than 3% with pyrithidium, diminazene and quinapyramine and about 13% with homidium. The limit of detection of ISMM in serum without prior extraction was 5.8 pmol/tube and within-assay and between-assay coefficients of variation were four and 14% respectively.
Kinabo
L D
LD
Department of Veterinary Pharmacology, University of Glasgow Veterinary School, Bearsden, Scotland, UK.
Bogan
J A
JA
D000818:Animals; D001667:Binding, Competitive; D002417:Cattle; D010617:Phenanthridines / Q000032:analysis* / Q000097:blood / Q000276:immunology; D011863:Radioimmunoassay / Q000662:veterinary*; D012709:Serum Albumin; D012756:Sheep / Q000276:immunology; D013997:Time Factors; D014344:Trypanocidal Agents / Q000032:analysis* / Q000097:blood
D010617:Phenanthridines; D012709:Serum Albumin; D014344:Trypanocidal Agents; C000702:isometamidium chloride