In vitro studies on the nucleocapsid-associated RNA polymerase of wheat rosette stunt virus.
RNA-dependent RNA polymerase activity was detected in both virion and nucleocapsid preparations of wheat rosette stunt virus, a plant rhabdovirus. The presence of nonionic detergent such as Nonidet P40 was essential for activity in reaction mixtures containing virions. The polymerase product was proved to be single-stranded RNA. By two-step controlled dissociation of the nucleocapsids, four subviral fractions (L protein, NS-N-RNA complex, NS protein, and N-RNA complex) were prepared. None of these fractions showed RNA polymerase activity when assayed individually. In experiments combining the various fractions, RNA synthesis was observed only when the L and NS proteins and the N-RNA complex were present in reaction mixtures.
Sun
W
W
Laboratory of Virology, Shanghai Institute of Biochemistry, Chinese Academy of Sciences.
Gong
Z X
ZX
D002213:Capsid / Q000302:isolation & purification; D010942:Plant Viruses / Q000201:enzymology* / Q000235:genetics; D012316:RNA Nucleotidyltransferases / Q000302:isolation & purification*; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics / Q000302:isolation & purification*; D014158:Transcription, Genetic; D014758:Viral Core Proteins / Q000302:isolation & purification
D014758:Viral Core Proteins; D012316:RNA Nucleotidyltransferases; D012324:RNA-Dependent RNA Polymerase