title: Isolation and primary structure of human peptide YY.
abstract: The isolation, primary structure and chemical synthesis of human peptide YY (PYY) are described. The peptide was purified from human colonic extracts using a chemical method which detected the C-terminal tyrosine amide structure of PYY. Human PYY consists of 36 amino acid residues and the complete amino acid sequence is: Tyr-Pro-Ile-Lys-Pro-Glu-Ala-Pro-Gly-Glu- Asp-Ala-Ser-Pro-Glu-Glu-Leu-Asn-Arg-Tyr-Tyr-Ala-Ser-Leu-Arg-His-Tyr-Leu- Asn-Leu-Val-Thr-Arg-Gln-Arg-Tyr-NH2. The differences between the structures of porcine and human PYY are at positions 3 (Ala/Ile replacement) and 18 (Ser/Asn). Synthetic human PYY prepared using a solid-phase synthetic technique was found to be structurally identical to the natural peptide.
authors.affiliation: Department of Psychiatry and Behavioral Sciences, Stanford University School of Medicine, California.
authors.forename: K
authors.initials: K
authors.lastname: Tatemoto
authors.forename: I
authors.initials: I
authors.lastname: Nakano
authors.forename: G
authors.initials: G
authors.lastname: Makk
authors.forename: P
authors.initials: P
authors.lastname: Angwin
authors.forename: M
authors.initials: M
authors.lastname: Mann
authors.forename: J
authors.initials: J
authors.lastname: Schilling
authors.forename: V L
authors.initials: VL
authors.lastname: Go
mesh_terms: D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D003106:Colon / Q000032:analysis; D005768:Gastrointestinal Hormones / Q000138:chemical synthesis / Q000302:isolation & purification*; D006801:Humans; D008969:Molecular Sequence Data; D019894:Peptide YY; D010455:Peptides / Q000138:chemical synthesis / Q000302:isolation & purification*
chemical_list: D005768:Gastrointestinal Hormones; D010455:Peptides; D019894:Peptide YY