title: Structural and functional relationships between nuclear bodies and the nucleolus-DNA body complex in the oocyte of Amphiporus lactifloreus.
abstract: The localization, structure and function of two types of nuclear bodies have been investigated by cytological and cytochemical electron microscopy methods in oocytes from the Hoplonemertean, Amphiporus lactifloreus. Type I nuclear bodies differentiate in contact with the nucleolus-DNA body complex, whereas type II nuclear bodies develop close to the diplotenic chromosomal axes. The structure of type I and type II spherical nuclear bodies, 4-5 micron in width, results from the association of a fibrillar reticulum with some dense included regions. The cytochemical findings following the use of osmium-ammine reaction for DNA and silver reaction for NOR proteins support the hypothesis that type II nuclear bodies, derived from the extranucleolar area, as well as type I nuclear bodies, derived from the nucleolar complex, may be involved in ribosomal biogenesis.
authors.affiliation: Laboratoire de Biologie Cellulaire et Générale, Université de Champagne, Reims, France.
authors.forename: G
authors.initials: G
authors.lastname: Rué
authors.forename: J
authors.initials: J
authors.lastname: Bierne
mesh_terms: D000818:Animals; D002466:Cell Nucleolus / Q000502:physiology / Q000648:ultrastructure; D002467:Cell Nucleus / Q000502:physiology / Q000648:ultrastructure; D004247:DNA / Q000235:genetics*; D006376:Helminths / Q000648:ultrastructure*; D006651:Histocytochemistry; D008854:Microscopy, Electron; D009687:Nuclear Proteins / Q000032:analysis; D009865:Oocytes / Q000648:ultrastructure*; D014158:Transcription, Genetic
chemical_list: D009687:Nuclear Proteins; D004247:DNA