Fertilization of Chinese hamster ova in vitro and in vivo and their subsequent development in culture.
Oocytes from superovulated Chinese hamsters can be fertilized in vitro using the culture medium BWW (70% of 112 ova) or a modified BWW designated as MBWW (76% of 122 ova) when either medium is supplemented with 4 mg/ml of bovine serum albumin. Ova fertilized in vitro will also cleave to the 2-cell stage in either medium (52% in BWW, 87% in MBWW), but fail to develop any further in culture. Oocytes fertilized in vivo and recovered at the late 2-cell or early 4-cell stages from females on Day 3 of pregnancy have the capacity to develop into expanded blastocysts in MBWW. When early embryos that developed into morulae and early blastocyts in culture were transferred to surrogate females, eight normal young were born.
Parkening
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Department of Anatomy, University of Texas Medical Branch, Galveston 77550-2772.
Cisneros
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D000818:Animals; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D003470:Culture Media; D004624:Embryo Transfer; D005260:Female; D005306:Fertilization; D005307:Fertilization in Vitro; D013480:Superovulation; D015053:Zygote
D003470:Culture Media