Regulation of intracellular pH in rat uterine smooth muscle, studied by 31P NMR spectroscopy.
Intracellular pH (pHi) affects smooth muscle function, yet little is known concerning its regulation. I have therefore investigated pH regulation in rat uterus, using 31P-NMR spectroscopy. A change in extracellular pH(pHe) of 1 pH unit (7.4 to 6.4) elicited a 0.29 change in pHi; smaller changes in pHe were accompanied by proportionately smaller changes in pHi. The pH changes were reversible. There was no fall of uterine ATP or phosphocreatine during the pH changes.
Wray
S
S
MRC Biomedical NMR Centre, London, U.K.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D005110:Extracellular Space / Q000378:metabolism; D005260:Female; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D009130:Muscle, Smooth / Q000378:metabolism*; D010710:Phosphates / Q000378:metabolism; D010725:Phosphocreatine / Q000378:metabolism; D011247:Pregnancy; D051381:Rats; D011919:Rats, Inbred Strains; D014599:Uterus / Q000378:metabolism*
D010710:Phosphates; D010725:Phosphocreatine; D000255:Adenosine Triphosphate