title: Effect of hydrocortisone on sialyltransferase activity in the rat small intestine during maturation. Changes along the villus-crypt axis and in fetal organ culture.
abstract: Sialyltransferase activity was assayed in rat intestinal cells isolated as fractions reflecting the villus-crypt axis of differentiation. In 13-day-old rats both endo- and exogenous sialyltransferase activity reached their maximum in undifferentiated crypt cells and their peaks overlapped. In contrast, sialyltransferase of the adult intestine was 4-fold lower than that of sucklings in the crypts, with slight tendency to be transferred to the villus cells. Hydrocortisone applied to 10-day-old rats caused three days later a precocious drop of sialyltransferase activity in the crypt cells. Unlike in vivo, glucocorticoid responsiveness was accompanied by increased sialyltransferase activity in fetal small intestine cultivated for 17 days.
authors.affiliation: Department of Membrane Transport, Czechoslovak Academy of Sciences, Prague.
authors.forename: J
authors.initials: J
authors.lastname: Kolínská
authors.forename: S
authors.initials: S
authors.lastname: Ivanov
authors.forename: H
authors.initials: H
authors.lastname: Chelibonova-Lorer
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D003907:Dexamethasone / Q000494:pharmacology; D005333:Fetus / Q000201:enzymology*; D006854:Hydrocortisone / Q000494:pharmacology*; D007421:Intestine, Small / Q000196:embryology / Q000201:enzymology / Q000254:growth & development*; D007583:Jejunum / Q000196:embryology / Q000201:enzymology; D008871:Microvilli / Q000201:enzymology; D019158:N-Acetylneuraminic Acid; D009924:Organ Culture Techniques; D051381:Rats; D012794:Sialic Acids / Q000378:metabolism; D012799:Sialyltransferases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D014018:Tissue Distribution; D000094802:beta-D-Galactoside alpha 2-6-Sialyltransferase
chemical_list: D012794:Sialic Acids; D003907:Dexamethasone; D012799:Sialyltransferases; D019158:N-Acetylneuraminic Acid; D006854:Hydrocortisone; D000094802:beta-D-Galactoside alpha 2-6-Sialyltransferase