Nucleoside analysis of DNA from Trypanosoma brucei and Trypanosoma equiperdum.
We have digested trypanosome DNA with a combination of pancreatic DNase I, nuclease P1 and bovine alkaline phosphatase and fractionated the resulting nucleosides on a Supelcosil LC-18-S column by high pressure liquid chromatography. We find less than 0.1% unusual nucleosides, both in Trypanosoma brucei and in a Trypanosoma equiperdum stock, in contrast to a previous report of an unusual nucleoside replacing dC at 1.3% of total nucleosides in T. equiperdum. Our results agree with previous suggestions that the modification of inactive telomeric expression sites for variant-specific surface glycoprotein genes in T. brucei only affects a very small fraction of the total DNA.
Crozatier
M
M
Division of Molecular Biology, The Netherlands Cancer Institute, Amsterdam.
De Brij
R J
RJ
Den Engelse
L
L
Johnson
P J
PJ
Borst
P
P
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000032:analysis* / Q000378:metabolism; D006868:Hydrolysis; D009705:Nucleosides / Q000032:analysis*; D014345:Trypanosoma / Q000235:genetics*; D014346:Trypanosoma brucei brucei / Q000235:genetics*
D009705:Nucleosides; D004247:DNA