In vitro interactions of gadolinium DOTA meglumine and gadolinium DTPA meglumine on hemostasis.
The interactions of two gadolinium complexes (Gd-DOTA meglumine and Gd-DTPA meglumine) with hemostatic function have been analyzed using: (1) coagulation reactions (extrinsic and intrinsic pathways and fibrinoformation) and (2) platelet function investigations (aggregation, release of Ca++ and ATP after stimulation with collagen 2.5 micrograms/mL). The data obtained with Gd-DTPA meglumine (Mgl) exhibited a significant increase of the intrinsic coagulation pathway and a delay in fibrinoformation, although there is no alteration of the effect of thrombin on fibrinogen (fibrinopeptid A determinations). Platelet aggregation and release are moderately modified. In contrast, Gd-DOTA Mgl exerts no effect on the coagulation system and only minor effects on platelet functions. It is suggested that at least one mechanism involves the complexation of ionized calcium because Gd-DTPA Mgl and Gd-DOTA Mgl complex, respectively, 45% and 23% of ionized calcium in plasma. However, other mechanisms such as an alteration of fibrin polymerization are not unlikely.
Corot
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C
INSERM U.37, Bron, France.
Belleville
J
J
Paul
J
J
Baguet
J
J
Amiel
M
M
Eloy
R
R
D003287:Contrast Media / Q000494:pharmacology*; D019786:Gadolinium DTPA; D006487:Hemostasis / Q000187:drug effects*; D006571:Heterocyclic Compounds / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D008279:Magnetic Resonance Imaging; D008536:Meglumine / Q000494:pharmacology; D009942:Organometallic Compounds / Q000494:pharmacology*; D004369:Pentetic Acid / Q000494:pharmacology*
D003287:Contrast Media; D006571:Heterocyclic Compounds; D009942:Organometallic Compounds; D008536:Meglumine; D004369:Pentetic Acid; C050823:gadolinium 1,4,7,10-tetraazacyclododecane-N,N',N'',N'''-tetraacetate; D019786:Gadolinium DTPA