Chemical synthesis of DNA oligomers containing cytosine arabinoside.
The solid phase phospite triester synthesis of oligodeoxynucleotides containing cytosine arabinoside (araC) is described. A protected araC phosphoramadite was prepared for the introduction of araC residues at 5'termini and internucleotide positions in DNA oligomers. These oligomers were utilized to demonstrate the formation of correct 3'-5' linkages, to test for alkaline lability at the araC site, and to study the stability of duplexes containing araC-G base pairs. For the introduction of araC residues at 3' terminal positions, a protected derivative of araC was coupled to functionalized silica. This material was used to prepare a test oligomer which was characterized enzymatically.
Beardsley
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GP
Department of Pediatrics, Yale University School of Medicine, New Haven, CT 06510.
Mikita
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T
Klaus
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Nussbaum
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D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D003561:Cytarabine; D009691:Nucleic Acid Denaturation; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis*; D013696:Temperature
D009838:Oligodeoxyribonucleotides; D003561:Cytarabine