Stability and immunologic activity of immobilized heparinase and bilirubin oxidase.
Two model enzymes, heparinase and bilirubin oxidase, were immobilized via cyanogen bromide, tresyl chloride, epoxide, or carbodiimidazole activated natural and synthetic matrices. The supports released enzymes for 4-20 hr in vitro at 37 degrees C. The most stable linkage was formed by tresyl chloride and oxirane activated supports. No immune response was detected 11 or 21 days after single i.v. injection to New Zealand white rabbits. Acute exposure to the enzyme produced a weak positive response.
Comfort
A R
AR
Department of Chemical Engineering, Harvard-M.I.T., Boston 02139.
Mullon
C J
CJ
Koh
J
J
Albert
E
E
Tosone
C P
CP
Hall
J
J
Langer
R
R
D000818:Animals; D000917:Antibody Formation; D003488:Cyanogen Bromide; D004355:Drug Stability; D004800:Enzymes, Immobilized / Q000378:metabolism*; D019763:Heparin Lyase; D007093:Imidazoles; D007202:Indicators and Reagents; D010088:Oxidoreductases / Q000276:immunology / Q000378:metabolism*; D044925:Oxidoreductases Acting on CH-CH Group Donors; D011133:Polysaccharide-Lyases / Q000276:immunology / Q000378:metabolism*; D011817:Rabbits; D013450:Sulfones
D004800:Enzymes, Immobilized; D007093:Imidazoles; D007202:Indicators and Reagents; D013450:Sulfones; C032605:2,2,2-trifluoroethanesulfonyl chloride; D010088:Oxidoreductases; D044925:Oxidoreductases Acting on CH-CH Group Donors; C042401:bilirubin oxidase; D011133:Polysaccharide-Lyases; D019763:Heparin Lyase; D003488:Cyanogen Bromide