Production of IGF-II-related peptide by an anaplastic cell line (AT-3) established from the Dunning prostatic carcinoma of rats.
AT-3 cells, one of anaplastic cell lines established from the Dunning prostatic carcinoma of rats, were able to grow under serum-free conditions in a state of suspension detached from a substratum. Radioimmunoassay using monoclonal antibody against rat insulin-like growth factor II (IGF-II) revealed the presence of IGF-II-related peptide in acid-ethanol extracts of lyophilized serum-free media conditioned by AT-3 cells. The peptide contents in the culture media increased with increase in cell number; 71 ng at 3.0 X 10(6) cells and 449 ng at 4.6 X 10(7) cells. IGF-II-related peptide was hardly detectable in acid-ethanol extracts of AT-3 cells harvested after 13-days culture. These results indicate that AT-3 cells produce IGF-II-related peptide and may release it into the culture media.
Matuo
Y
Y
Department of Endocrinology, Kagawa Medical School, Japan.
Nishi
N
N
Tanaka
H
H
Sasaki
I
I
Isaacs
J T
JT
Wada
F
F
D000818:Animals; D002277:Carcinoma / Q000378:metabolism* / Q000473:pathology; D002455:Cell Division / Q000187:drug effects; D003470:Culture Media; D003907:Dexamethasone / Q000494:pharmacology; D007335:Insulin-Like Growth Factor II / Q000096:biosynthesis*; D008297:Male; D009362:Neoplasm Metastasis; D011471:Prostatic Neoplasms / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D013002:Somatomedins / Q000096:biosynthesis*; D014407:Tumor Cells, Cultured
D003470:Culture Media; D013002:Somatomedins; D007335:Insulin-Like Growth Factor II; D003907:Dexamethasone