title: Wheat germ agglutinin fracture-label of Golgi apparatus membranes in proliferating cells.
abstract: The thin section fracture-label technique has been recently used to analyze the distribution and compartmentalization of fully glycosylated components on intracellular membranes. Labelling with the lectin wheat germ agglutinin over the freeze-fractured membranes of Golgi apparatus in various secretory and non-secretory cells as well as in human peripheral lymphocytes was always very weak or absent even over the trans-most cisternae. In order to investigate if the labelling density may reflect the cellular activity in membrane biogenesis, we used in this study wheat germ agglutinin fracture-label of rapidly proliferating cells and mitogen-activated lymphocytes. Labelling over the fractured cisternae of the medial and trans portions of the Golgi apparatus was intense. Treatment with cycloheximide of proliferating cells induced a drastic reduction of the labelling over the Golgi cisternae.
authors.affiliation: Dipartimento di Medicina Sperimentale, Università degli Studi di Roma La Sapienza, Italy.
authors.forename: L V
authors.initials: LV
authors.lastname: Lotti
authors.forename: A
authors.initials: A
authors.lastname: Pavan
authors.forename: P
authors.initials: P
authors.lastname: Mancini
authors.forename: L
authors.initials: L
authors.lastname: Frati
authors.forename: M R
authors.initials: MR
authors.lastname: Torrisi
mesh_terms: D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D003513:Cycloheximide / Q000494:pharmacology; D005614:Freeze Fracturing / Q000379:methods*; D006023:Glycoproteins / Q000032:analysis*; D006031:Glycosylation; D006056:Golgi Apparatus / Q000032:analysis* / Q000187:drug effects / Q000648:ultrastructure; D006801:Humans; D008214:Lymphocytes / Q000032:analysis* / Q000187:drug effects / Q000648:ultrastructure; D008854:Microscopy, Electron; D014909:Wheat Germ Agglutinins
chemical_list: D006023:Glycoproteins; D014909:Wheat Germ Agglutinins; D003513:Cycloheximide