2-Hydroxyethylation of haemoglobin in man.
Exposure of humans to 2-hydroxyethylating agents, such as ethylene oxide, results in the formation of N-(2-hydroxyethyl)valine (HOEtVal) at the N-terminal amino acid of haemoglobin. A novel method using gas chromatography-mass spectrometry (GC-MS) has been used to monitor the presence of this adduct in smokers and control subjects, and dose-response relationships were investigated between HOEtVal in haemoglobin, number of cigarettes smoked per day and plasma cotinine levels.
Passingham
B J
BJ
MRC Toxicology Unit, Medical Research Council Laboratories, Carshalton, Surrey, UK.
Farmer
P B
PB
Bailey
E
E
Brooks
A G
AG
Yates
D W
DW
D003367:Cotinine / Q000097:blood; D008401:Gas Chromatography-Mass Spectrometry; D006454:Hemoglobins / Q000378:metabolism*; D006639:Histidine / Q000031:analogs & derivatives / Q000032:analysis; D006801:Humans; D012907:Smoking / Q000097:blood; D014633:Valine / Q000031:analogs & derivatives* / Q000032:analysis
D006454:Hemoglobins; C057442:2-hydroxyethylvaline; D006639:Histidine; C054241:3-(2-hydroxyethyl)histidine; D014633:Valine; D003367:Cotinine