Nuclear maturity and morphology of human spermatozoa selected by Percoll density gradient centrifugation or swim-up procedure.
The selection of motile human spermatozoa, from fertile and infertile semen samples was compared by using Percoll density gradient centrifugation or the swim-up procedure. Selected spermatozoa were evaluated according to their motility, % normal forms, nuclear maturity (aniline blue staining, acridine orange staining, ethidium bromide uptake and SDS nuclear decondensation). These methods showed differences between fertile and infertile men. The swim-up procedure, based on motility, resulted in greater proportions of motile spermatozoa and eliminated mainly tail abnormalities. Percoll gradient separation, based on density, selected oval-headed spermatozoa with good motility. Nuclear maturity level was improved by both methods but Percoll gradient separation generally resulted in spermatozoa with better nuclear maturity than those selected by the swim-up procedure.
Le Lannou
D
D
Unite de Biologie de la Reproduction, Cecos de l'ouest, Hotel Dieu, Rennes, France.
Blanchard
Y
Y
D002467:Cell Nucleus / Q000502:physiology; D002469:Cell Separation / Q000379:methods*; D002499:Centrifugation, Density Gradient; D006801:Humans; D007315:Insemination, Artificial / Q000379:methods*; D008297:Male; D013081:Sperm Motility; D013094:Spermatozoa / Q000166:cytology*