title: Colony growth of normal and neoplastic cells in various concentrations of methylcellulose.
abstract: To assess the semisolid character of methylcellulose (MC) and its ability to prevent cell migration and aggregation in clonogenic assays, we studied the influence of various concentrations of MC (0.7%-1.26%) on colony growth of neoplastic cell lines, normal bone marrow cells, and hairy cell leukemia (HCL). All cell lines (K562, HL-60, JOK-1, Daudi, and BB3, an IgM-kappa B-cell line) showed a prominent decrease in colony numbers and remarkable changes in colony morphology at rising MC concentrations, whereas no such influence could be demonstrated for HCL, mixed lineage colony-forming units (CFU-GEMM), granulocyte-macrophage CFU (CFU-GM), erythroid burst-forming units (BFU-E), and erythroid CFU (CFU-E). Despite a decrease in colony numbers at high MC concentrations, some cell lines showed a sustained proliferation as measured by growth index calculations and bromodeoxyuridine (BrdUrd) incorporation. This indicates that at certain MC concentrations colony formation is not always a reflection of proliferation. BrdUrd incorporation yielded an extremely low proliferation capacity for HCL. It is likely that HCL cells, which strongly aggregate, formed pseudo-colonies in spite of high MC concentrations.
authors.affiliation: Laboratory of Experimental Hematology, Leiden University Medical Center, The Netherlands.
authors.forename: H C
authors.initials: HC
authors.lastname: Kluin-Nelemans
authors.forename: H W
authors.initials: HW
authors.lastname: Hakvoort
authors.forename: J H
authors.initials: JH
authors.lastname: Jansen
authors.forename: N
authors.initials: N
authors.lastname: Duinkerken
authors.forename: J F
authors.initials: JF
authors.lastname: van den Burgh
authors.forename: J H
authors.initials: JH
authors.lastname: Falkenburg
authors.forename: R
authors.initials: R
authors.lastname: Willemze
mesh_terms: D001854:Bone Marrow Cells; D002452:Cell Count; D002460:Cell Line; D003114:Colony-Forming Units Assay; D003470:Culture Media; D006801:Humans; D007943:Leukemia, Hairy Cell / Q000473:pathology*; D008747:Methylcellulose / Q000494:pharmacology*
chemical_list: D003470:Culture Media; D008747:Methylcellulose