Application of bromodeoxyuridine incorporation measurements to the determination of cell distribution within the S phase of the cell cycle.
Flow cytofluorimetric measurement of incorporated bromodeoxyuridine, using a double-stained cell population, allows the determination of the distribution of cells along the cell cycle. We have developed a simple computer program for the direct treatment of 64 x 64 channel histograms. This analysis appears to provide interesting data about the distribution of cells in the various phases of the cell cycle, namely the S phase. Two examples have been chosen to illustrate possible fields for the application of such a program. Comparison of two cell lines such as friend murine erythroleukemia cells (MELC) and fibroblasts FR3T3 cells has shown that this analysis can be used for cell-cycle characterization of a given cell line. The program also allows the differential analysis of cell distribution along the cell cycle as a function of a given parameter. This possibility has been applied to study the variation of cell-cycle parameters as a function of the time of induced differentiation of MELC and reveals changes in the distribution of the cells along the various phases of the cell cycle, namely in the S phase.
Khochbin
S
S
Unite Inserm 309, Departement de Recherche Fondamentale, CEN, Grenoble, France.
Chabanas
A
A
Albert
P
P
Albert
J
J
Lawrence
J J
JJ
D000818:Animals; D001973:Bromodeoxyuridine; D002453:Cell Cycle; D002460:Cell Line; D001330:Electronic Data Processing; D005434:Flow Cytometry / Q000379:methods*; D004915:Leukemia, Erythroblastic, Acute; D051379:Mice; D014407:Tumor Cells, Cultured / Q000166:cytology*
D001973:Bromodeoxyuridine