DNA-binding protein(s) interacts with a conserved nonameric sequence in the upstream regions of wheat histone genes.
A nuclear protein(s), HBP-2, that binds to the upstream region of the wheat histone H4 gene was identified from a fractionated nuclear extract of wheat germ by DNase I footprinting. The DNase I-protected region contained the conserved nonameric motif, CATCCAACG. Cross-competition experiments that used the mobility shift assay showed that this nuclear protein(s) binds specifically to the upstream sequence that has been postulated to be a cis element of the wheat H3 gene. Our findings suggest that this DNA-binding protein(s) may be a trans-acting factor in the regulation of the transcription of wheat histone genes.
Kawata
T
T
Department of Biology, Faculty of Science, Hokkaido University, Sapporo, Japan.
Nakayama
T
T
Mikami
K
K
Tabata
T
T
Takase
H
H
Iwabuchi
M
M
D001483:Base Sequence; D004247:DNA / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism*; D003850:Deoxyribonuclease I; D005796:Genes; D008969:Molecular Sequence Data; D010944:Plants / Q000235:genetics* / Q000378:metabolism; D014908:Triticum / Q000235:genetics / Q000378:metabolism
D004268:DNA-Binding Proteins; D004247:DNA; D003850:Deoxyribonuclease I