A genetic analysis of the adenine phosphoribosyl transferase locus in Chinese hamster V79-AP4 cells: relevance to mutagenesis studies.
The chromosomal location of the autosomal locus aprt has been investigated in the permanent Chinese hamster cell line V79-AP4 by standard somatic cell genetics methodologies. Aprt is functionally dizygous in V79-AP4 and the 2 alleles map on 2 chromosome 3 homologs, in agreement with the chromosome assignment of the gene in Chinese hamster primary cells. Chromosome G-banding and a Southern blot analysis of V79-AP4 DNA, using as a probe the cloned Chinese hamster aprt gene, have not revealed any structural alteration at either of the 2 aprt alleles. One of the chromosomes 3 has, however, a terminal deletion in its long arm and is therefore morphologically marked. These findings could make V79-AP4 an interesting cell system for the study of mutational mechanisms at the aprt locus in Chinese hamster.
Colella
C M
CM
Istituto di Mutagenesi e Differenziamento CNR, Pisa, Italy.
Simi
S
S
Van Boxel
T
T
Talarico
D
D
Della Valle
G
G
Carrozza
M L
ML
Fratta
D
D
Mariani
T
T
Piras
A
A
Simili
M
M
D015075:2-Aminopurine / Q000031:analogs & derivatives / Q000494:pharmacology; D000228:Adenine Phosphoribosyltransferase / Q000235:genetics*; D000818:Animals; D015139:Blotting, Southern; D002460:Cell Line; D002871:Chromosome Banding; D002874:Chromosome Mapping; D006224:Cricetinae / Q000235:genetics*; D003412:Cricetulus / Q000235:genetics*; D015342:DNA Probes; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000235:genetics; D009152:Mutagenicity Tests / Q000379:methods*; D010430:Pentosyltransferases / Q000235:genetics*
D015342:DNA Probes; D007527:Isoenzymes; D015075:2-Aminopurine; C007300:2,6-diaminopurine; D007770:L-Lactate Dehydrogenase; D010430:Pentosyltransferases; D000228:Adenine Phosphoribosyltransferase