title: Hepatic DNA adduct formation in rats treated with benzo[f]quinoline.
abstract: The formation of hepatic DNA adducts in male Sprague-Dawley rats following i.p. administration of benzo[f]quinoline (BfQ) was examined using a 32P-post-labeling assay. BfQ exhibited a low binding (11-27 amol adducts/microgram DNA) to liver DNA. Two BfQ-nucleoside adducts (one major and one minor) were detected. The BfQ-DNA adducts formed in vivo were chromatographically distinct from the adducts formed by the reaction of calf thymus DNA in vitro with BfQ-5,6-oxide, syn-7 beta,8 alpha-dihydroxy-9 beta,10 beta-epoxy-7,8,9,10-tetrahydroBfQ, anti-9 alpha,10 beta-dihydroxy-7 alpha,8 alpha-epoxy-7,8,9,10-tetrahydroBfQ, or anti-7 beta,8 alpha-dihydroxy-9 alpha,10 alpha-epoxy-7,8,9,10-tetrahydroBfQ-N- oxide. These results suggest that the bay-region diol epoxide of BfQ, unlike the bay-region diol epoxide derivatives of polynuclear aromatic hydrocarbons, is not involved in the covalent binding of BfQ to DNA.
authors.affiliation: Great Lakes Laboratory, State University of New York College, Buffalo 14222.
authors.forename: H C
authors.initials: HC
authors.lastname: Sikka
authors.forename: C
authors.initials: C
authors.lastname: Kandaswami
authors.forename: S
authors.initials: S
authors.lastname: Kumar
authors.forename: J P
authors.initials: JP
authors.lastname: Rutkowski
authors.forename: S K
authors.initials: SK
authors.lastname: Dubey
authors.forename: K
authors.initials: K
authors.lastname: Earley
authors.forename: R C
authors.initials: RC
authors.lastname: Gupta
mesh_terms: D000818:Animals; D004247:DNA / Q000378:metabolism*; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D011804:Quinolines / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D011804:Quinolines; C039108:benzo(f)quinoline; D004247:DNA