The measurement of plasma vitellogenin levels in a marine teleost, the spotted seatrout (Cynoscion nebulosus) by homologous radioimmunoassay.
1. Vitellogenin was purified from plasma of estrogen-treated spotted seatrout (Cynoscion nebulosus) and was shown to be a protein of approximately 200,000 mol. wt. 2. Antisera were raised against the isolated protein and were used to develop a radioimmunoassay for spotted seatrout vitellogenin. 3. The substance measured was shown to be female-specific, hepatically-derived, estrogen-inducible and immunologically related to oocyte yolk proteins. 4. Vitellogenin levels in the plasma of female spotted seatrout were shown to rise dramatically during the period of ovarian recrudescence.
Copeland
P A
PA
University of Texas at Austin, Marine Science Institute, Port Aransas 78373-1267.
Thomas
P
P
D000818:Animals; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D005399:Fishes / Q000097:blood*; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism; D008970:Molecular Weight; D011863:Radioimmunoassay; D014819:Vitellogenins / Q000096:biosynthesis / Q000097:blood*
D014819:Vitellogenins; D004958:Estradiol