Independent effects of diet and nutritional status on apoprotein B gene expression in rabbit.
The separate effects of dietary composition and time of nutrient intake on apoprotein B (apo B) gene expression in rabbit intestine and liver were determined. After an overnight fast, there were insignificant effects of the long-term consumption of a diet containing saturated fat on the tissue levels of apo B mRNA, although plasma cholesterol levels were elevated as compared to those of animals consuming a diet containing unsaturated fat. However, when the animals were studied 2 hours after receiving a gastric tube feeding of a portion of their test diets after an overnight fast, there was a twofold increase in the intestinal apo B mRNA level and a similar increase in the transcriptional activity of the apo B gene independent of dietary composition. We conclude that, with the diets we used, nutrient intake alone has, in rabbits, a major effect on apo B gene expression at the level of transcription. This factor should be taken into account when designing studies of intestinal gene expression.
Fisher
E A
EA
Department of Physiology and Biochemistry, Medical College of Pennsylvania, Philadelphia 19129.
Anbari
A
A
Klurfeld
D M
DM
Kritchevsky
D
D
D000199:Actins / Q000235:genetics; D000818:Animals; D001055:Apolipoproteins B / Q000235:genetics*; D002784:Cholesterol / Q000097:blood; D004032:Diet; D004041:Dietary Fats / Q000008:administration & dosage; D005786:Gene Expression Regulation; D007421:Intestine, Small / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D009752:Nutritional Status; D012333:RNA, Messenger / Q000302:isolation & purification; D011817:Rabbits
D000199:Actins; D001055:Apolipoproteins B; D004041:Dietary Fats; D012333:RNA, Messenger; D002784:Cholesterol