Sedimentation coefficient and minimum molecular weight of extracellular hemoglobin of Glossoscolex paulistus (Oligochaeta).
A new procedure for the determination of the sedimentation coefficient in the 50-1000 S range was designed and tested. The characteristics of this protocol are: the use of density gradients self-generated by osmosedimentation and the use of a low-speed centrifuge and of visual monitoring of the sedimenting zone. This procedure was used to determine the sedimentation coefficient of erythrocruorin from Glossoscolex paulistus. The value obtained, S20, omega = 58 S, corresponds to a MW of 3.1 x 10(6) Daltons. The minimum MW (heme), determined by the pyridine-hemochromogen method, was 25,250 Da.
Costa
M C
MC
Departamento de Química, Universidade Federal do Maranhão, São Luís, Brasil.
Bonafé
C F
CF
Meirelles
N C
NC
Galembeck
F
F
D000818:Animals; D002499:Centrifugation, Density Gradient; D004902:Erythrocruorins / Q000032:analysis*; D006454:Hemoglobins / Q000032:analysis*; D008970:Molecular Weight; D009835:Oligochaeta
D004902:Erythrocruorins; D006454:Hemoglobins