title: Erythrocyte deformability and segmental pulmonary vascular resistance: osmolarity and heat treatment.
abstract: The site and nature of change in resistance to blood flow in canine left lung lobe preparation after changes in blood viscosity were assessed by using the arterial and venous occlusion (AVO) technique and the vascular pressure-flow relationship. Blood viscosity was changed by erythrocyte (RBC) shrinkage and swelling with hypertonic and hypotonic NaCl solutions and by RBC membrane rigidification with heat treatment (49 degrees C for 1 h). The results show that although all three methods of changing blood viscosity increased the pulmonary vascular resistance (PVR) by 15-50%, the site and nature of the change in PVR were different in each case. The AVO data showed that the increase in PVR with heat treatment of RBC's was due entirely (100%) to increased resistance of the middle microvascular segment, whereas deviation from normal osmolarity potentiated the resistance in arterial, middle, and venous segments. By examining the effect of osmolarity in plasma-perfused lobes, it was possible to separate the increase in PVR due to changes in RBC deformability from those due to other factors. The increase in arterial and venous resistances with hypertonic solution was attributed in part (approximately 50%) to factors other than RBC's; however, the increase in middle resistance was entirely due to RBC crenation. The increase in arterial and venous resistances with hypotonic solutions was small and was apparently caused by factors other than RBC swelling, whereas the increase in middle resistance was partially (approximately 50%) due to RBC swelling and partially to other factors (e.g., endothelial cell hydration).(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Physiology, McGill University, Montreal, Quebec, Canada.
authors.forename: T S
authors.initials: TS
authors.lastname: Hakim
mesh_terms: D000818:Animals; D001794:Blood Pressure; D001809:Blood Viscosity; D004285:Dogs; D004907:Erythrocyte Deformability; D004909:Erythrocyte Indices; D006358:Hot Temperature; D008168:Lung / Q000098:blood supply*; D009994:Osmolar Concentration; D011651:Pulmonary Artery / Q000502:physiology; D011667:Pulmonary Veins / Q000502:physiology; D012965:Sodium Chloride; D012996:Solutions; D014655:Vascular Resistance
chemical_list: D012996:Solutions; D012965:Sodium Chloride