Alcohol abuse increases the lipid structural order in human erythrocyte membranes. A steady-state and time-resolved anisotropy study.
The effect of ethanol abuse on the lipid ordering of the human erythrocyte membranes was studied by steady-state and time-resolved fluorescence anisotropy measurements of DPH and its polar analogue TMA-DPH, which probe different membrane regions. Steady-state anisotropy values with DPH as a probe were slightly but significantly increased (+3%) in erythrocyte membranes from alcoholic patients. A resistance to the ethanol fluidizing effect was evidenced in these membranes with DPH and TMA-DPH. No difference in the probe lifetimes was detected between the control and the alcoholic subjects. In the alcoholic patients as compared to the healthy controls, the residual anisotropy for DPH was significantly increased (+7%) corresponding to an increase in the orientational order parameter of 4%; a decrease of the apparent correlation time value was also observed. Nevertheless, no differences between the two erythrocyte populations were observed with TMA-DPH.
Beaugé
F
F
INSERM U.26, Hôpital Fernand Widal, Paris, France.
Gallay
J
J
Stibler
H
H
Borg
S
S
D000328:Adult; D000437:Alcoholism / Q000378:metabolism*; D004161:Diphenylhexatriene; D004910:Erythrocyte Membrane / Q000032:analysis*; D005454:Fluorescence Polarization; D006801:Humans; D008297:Male; D008563:Membrane Lipids / Q000032:analysis*; D008875:Middle Aged
D008563:Membrane Lipids; D004161:Diphenylhexatriene