Effect of treatment of isolated mitochondrial membranes with phosphatidylinositol-cholesterol liposomes on the biosynthesis and composition of polyglycerophosphatides.
Interaction between mitochondria isolated from rat liver and [14C]phosphatidylinositol-cholesterol (2:1 molar ratio) liposomes was studied by following the level of biosynthesis and the composition of mitochondrial [3H]polyglycerophosphatides. After treatment with liposomes, biosynthesis decreased by about 30 per cent and there was a three-fold increase in biosynthesized [3H]phosphatidylglycerolphosphate. The effect of adding proteoliposomes (consisting of mitochondrial protein and liposomes) to mitochondria that had previously been treated with liposomes was also studied in respect to the composition of [3H]polyglycerophosphatides. The results are discussed in relation to the biological membrane-liposome interaction.
Stuhne-Sekalec
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Department of Clinical Biochemistry, Banting Institute, Faculty of Medicine, University of Toronto, Ontario, Canada.
Stanacev
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NZ
D000818:Animals; D002784:Cholesterol; D005994:Glycerophosphates / Q000096:biosynthesis*; D066298:In Vitro Techniques; D008081:Liposomes; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism*; D010716:Phosphatidylinositols / Q000008:administration & dosage*; D051381:Rats
D005994:Glycerophosphates; D008081:Liposomes; D010716:Phosphatidylinositols; D002784:Cholesterol