Detection in human cells of alkylated macromolecules attributable to exposure to nitrosamines.
Various methods for detecting DNA alkylation adducts are described briefly, with emphasis on immunoassays using antibodies against O6-methyldeoxyguanosine (O6-medGua), O4-methylthymidine (O4-meThy) and 7-methyldeoxyguanosine (7-medGua). The application of these methods to epidemiological studies is discussed, and results obtained so far on the presence of DNA alkylation adducts in human tissues are presented.
Montesano
R
R
International Agency for Research on Cancer, Lyon, France.
Brésil
H
H
Degan
P
P
Martel-Planche
G
G
Serres
M
M
Wild
C P
CP
D000478:Alkylation; D004247:DNA / Q000378:metabolism*; D004784:Environmental Monitoring; D006801:Humans; D007118:Immunoassay; D009369:Neoplasms / Q000139:chemically induced; D009602:Nitrosamines / Q000378:metabolism*
D009602:Nitrosamines; D004247:DNA