title: Direct determination of the blood concentration of halogenated anesthetic agents by gas chromatography.
abstract: The direct determination by gas chromatography of blood levels of anesthetic agents has been difficult because of the water content of blood. In the present study, the method of Yokota et al. (1967) was modified by improving the packing materials of the column, the blood sample vaporizer and the flow-path during analysis. As a result, accurate and reproducible determination of halothane, enflurane and isoflurane dissolved in blood was achieved. With this system, blood in which halothane, enflurane and isoflurane had been dissolved could be analyzed without changing the column between samples. Moreover, each sample was prepared in less than 10 min, and more than 100 consecutive determinations could be made with excellent reproducibility. The coefficient of variation was less than 3.8%.
authors.affiliation: Department of Anesthesiology and Resuscitology, Okayama University Medical School, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Yamada
authors.forename: Y
authors.initials: Y
authors.lastname: Hirai
authors.forename: S
authors.initials: S
authors.lastname: Sakano
authors.forename: M
authors.initials: M
authors.lastname: Kosaka
authors.forename: K
authors.initials: K
authors.lastname: Tada
authors.forename: S
authors.initials: S
authors.lastname: Furutani
authors.forename: F
authors.initials: F
authors.lastname: Kosaka
mesh_terms: D002849:Chromatography, Gas / Q000379:methods*; D004737:Enflurane / Q000097:blood*; D006221:Halothane / Q000097:blood*; D006801:Humans; D007530:Isoflurane / Q000097:blood*; D013696:Temperature
chemical_list: D004737:Enflurane; D007530:Isoflurane; D006221:Halothane