title: GC/MS analysis of phencyclidine acid metabolite in human urine.
abstract: Available methods for determining PCP use are based on the presence of the parent drug in urine. PCP, however, is very potent and is extensively metabolized; it is therefore present in urine in only small quantities. This work was undertaken to determine whether an amino acid metabolite of PCP, 5-(N-(1'-phenylcyclohexyl)amino)pentanoic acid, can be used to determine PCP use. A solid phase adsorption technique was developed to extract the amino acid metabolite from urine. Recovery averaged 93%, and subsequent GC/MS analysis was free from interference. Analysis of 67 urine samples demonstrated that the amino acid metabolite exists in human urine in significant quantities.
authors.affiliation: ElSohly Laboratories, Oxford, Mississippi 38655.
authors.forename: M A
authors.initials: MA
authors.lastname: elSohly
authors.forename: T L
authors.initials: TL
authors.lastname: Little
authors.forename: J M
authors.initials: JM
authors.lastname: Mitchell
authors.forename: B D
authors.initials: BD
authors.lastname: Paul
authors.forename: L D
authors.initials: LD
authors.lastname: Mell
authors.forename: J
authors.initials: J
authors.lastname: Irving
mesh_terms: D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D010622:Phencyclidine / Q000031:analogs & derivatives* / Q000378:metabolism / Q000652:urine
chemical_list: C031090:5-(N-(1'-phenylcyclohexyl)amino)pentanoic acid; D010622:Phencyclidine