Brush border membrane vesicles formed from human duodenal biopsies exhibit Na+-dependent concentrative L-leucine and D-glucose uptake.
The human duodenum actively transports L-leucine and D-glucose under Na+ gradient conditions as demonstrated by uptake studies using brush border membrane vesicles from organ donor duodenum. Brush border membrane vesicles formed from peroral duodenal biopsies likewise demonstrate Na+ dependent concentrative uptake of D-glucose and L-leucine. This is the first demonstration of active transport processes in human duodenum. A simple microvesiculation method to form these vesicles is described as well as its potential application to clinical medicine in studying diseases of defective intestinal transport.
Harig
J M
JM
Gastroenterology Division, Medical College of Wisconsin, Milwaukee.
Soergel
K H
KH
Barry
J
J
Ramaswamy
K
K
D001692:Biological Transport / Q000187:drug effects; D004386:Duodenum / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism*; D007583:Jejunum / Q000378:metabolism; D007700:Kinetics; D007930:Leucine / Q000378:metabolism*; D008871:Microvilli / Q000187:drug effects / Q000378:metabolism*; D011188:Potassium / Q000494:pharmacology; D012964:Sodium / Q000494:pharmacology*
D012964:Sodium; D007930:Leucine; D005947:Glucose; D011188:Potassium