title: DNA base composition of filarial nematodes.
abstract: We have determined the molar content of guanine + cytosine (GC content) of DNA of the filarial nematode (Brugia malayi, Brugia pahangi and Dirofilaria imitis) and of the free-living soil nematodes Caenorhabditis elegans and have analysed the DNA for the presence of methylcytosine. Two independent methods, thermal denaturation and direct analysis of base content by HPLC following enzymatic hydrolysis, reveal that the GC content of filarial nematodes is 26-28%. We have been unable to find methylcytosine in the DNA of B. malayi.
authors.affiliation: Department of Pathology, Albert Einstein College of Medicine, Bronx, New York 10461.
authors.forename: N
authors.initials: N
authors.lastname: Rothstein
authors.forename: T J
authors.initials: TJ
authors.lastname: Stoller
authors.forename: T V
authors.initials: TV
authors.lastname: Rajan
mesh_terms: D000818:Animals; D001482:Base Composition; D002009:Brugia / Q000032:analysis*; D002107:Caenorhabditis / Q000032:analysis*; D003596:Cytosine / Q000032:analysis; D004247:DNA / Q000032:analysis*; D004183:Dirofilaria immitis / Q000032:analysis*; D005370:Filarioidea / Q000032:analysis*; D006147:Guanine / Q000032:analysis; D007700:Kinetics
chemical_list: D006147:Guanine; D003596:Cytosine; D004247:DNA