A DNA-modification methylase from Bacillus stearothermophilus V.
A type II modification methylase (M BstVI) was partially purified from the thermophilic bacterium Bacillus stearothermophilus V. The methylase catalyses the transfer of methyl groups from S-adenosyl-L-methionine to unmodified double-stranded DNA. The product of methylation was identified by paper chromatography as N6-methyladenine. Since M BstVI protects DNA against cleavage by BstVI and XhoI restriction endonucleases, it follows that it methylates the adenine residue in the sequence 5'-C-T-C-G-A-G-3'.
Barra
R
R
Laboratorio de Biologia Molecular, Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Chile, Santiago.
Chiong
M
M
González
E
E
Vásquez
C
C
D004247:DNA / Q000378:metabolism; D001411:Geobacillus stearothermophilus / Q000201:enzymology* / Q000254:growth & development; D007700:Kinetics; D008745:Methylation; D009994:Osmolar Concentration; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific) / Q000302:isolation & purification / Q000378:metabolism*; D013696:Temperature
D004247:DNA; C057988:DNA modification methylase BstVI; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific)