Rabbit and rat hepatic lectins have two sugar-combining sites per monomeric unit.
Binding characteristics of the galactose/N-acetylgalactosamine-specific, hepatic lectins of rabbit and rat were studied using small, high-affinity ligands containing two and three N-acetylgalactosamine residues per molecule [Lee, R. T. and Lee, Y. C. (1987) Glycoconjugate J. 4, 317-328]. These N-acetylgalactosamine cluster ligands have the receptor-ligand dissociation constants in nanomolar range, so that the lectin-ligand interaction can easily studied by an equilibrium (gel chromatography) or non-equilibrium (fast filtration assay) method. The results suggest that there exist on the average two N-acetylgalactosamine-combining sites per monomeric unit of both the rabbit and rat lectins.
Lee
R T
RT
Department of Biology, Johns Hopkins University, Baltimore, MD 21218.
Lee
Y C
YC
D000818:Animals; D002236:Carbohydrate Conformation; D037102:Lectins / Q000032:analysis*; D008099:Liver / Q000032:analysis*; D011817:Rabbits; D051381:Rats
D037102:Lectins