title: The effect of various antibacterial preparations on the in vitro morphology and chemotactic response of equine neutrophils.
abstract: Two independent assay systems were used to study the effect of three antibacterial preparations on in vitro morphology and chemotaxis of equine neutrophils. Incubation of neutrophils with high (200 micrograms/ml) and medium (20 micrograms/ml) concentrations of neomycin impaired their response to standard chemoattractants. Trimethoprim/sulfadoxine (0.4/2.0 micrograms/ml-40/200 micrograms/ml) and benzylpenicillin (0.25-25 micrograms/ml) had no effect. Neutrophils collected from geldings 2 and 24 h after neomycin (5 mg/kg) administration had impaired responses to standard chemoattractants. Benzylpenicillin (13.2 mg/kg) had no effect.
authors.affiliation: Department of Surgery and Obstetrics, Royal Veterinary College, Hawkshead Campus, Hatfield, Hertfordshire, U.K.
authors.forename: J F
authors.initials: JF
authors.lastname: Pycock
authors.forename: W E
authors.initials: WE
authors.lastname: Allen
authors.forename: D J
authors.initials: DJ
authors.lastname: Porter
authors.forename: E H
authors.initials: EH
authors.lastname: Boyd
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology*; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D004338:Drug Combinations; D006736:Horses / Q000097:blood*; D008297:Male; D009355:Neomycin / Q000494:pharmacology; D009504:Neutrophils / Q000032:analysis / Q000166:cytology / Q000187:drug effects*; D010400:Penicillin G / Q000494:pharmacology; D013413:Sulfadoxine / Q000494:pharmacology; D013997:Time Factors; D014295:Trimethoprim / Q000494:pharmacology
chemical_list: D000890:Anti-Infective Agents; D004338:Drug Combinations; D013413:Sulfadoxine; D014295:Trimethoprim; D009355:Neomycin; D010400:Penicillin G