title: Analysis of commercial samples of acridine orange using high-performance liquid chromatography.
abstract: We analyzed commercial samples of acridine orange using reverse-phase high-performance liquid chromatography. The mobile phase of 90:10:2.5 acetonitrile:de-ionized water:pentane sulfonic acid gave baseline separations of components of acridine orange samples in 15 min. Many of the samples were fairly homogeneous; the absorbance due to the acridine orange component ranged from 83-97%. Among the 11 samples tested, one was not acridine orange, reputedly different samples were identical, and the principal component of one sample was not acridine orange.
authors.affiliation: Department of Anatomical Sciences and Neurobiology, University of Louisville, Kentucky 40292.
authors.forename: R D
authors.initials: RD
authors.lastname: Paulsen
authors.forename: G S
authors.initials: GS
authors.lastname: Nettleton
mesh_terms: D000097:Acetonitriles / Q000032:analysis; D000165:Acridine Orange / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D013056:Spectrophotometry, Ultraviolet; D013451:Sulfonic Acids / Q000032:analysis; D014867:Water / Q000032:analysis
chemical_list: D000097:Acetonitriles; D013451:Sulfonic Acids; D014867:Water; D000165:Acridine Orange; C032159:acetonitrile