Isolation of in vivo radiolabeled proteoglycans from rat lung.
Sulfated macromolecules of rat lung tissue were labeled in vivo with 35SO4 and extracted with a solution of 7 M urea containing 0.4% Triton X-100. DEAE-Sephacel chromatography separated sulfated macromolecules into three pools. Pool I consisted of high-molecular-weight, low-density sulfated glycoprotein, probably of mucous secretion origin. Pool II contained a mixture of proteoheparan sulfate and proteodermatan sulfate, together with core protein-free heparan sulfate chains. Pool III was very heterogeneous; its resolution into at least four proteoglycan species was achieved by CsCl density gradient centrifugation. Those included two (high- and low-density) species of proteoheparan sulfate, high-density proteochondroitin sulfate, and medium-density (1.45 less than rho less than 1.55 g/ml) proteodermatan sulfate.
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Institute of Hygiene and Epidemiology, Centre of Industrial Hygiene and Occupational Diseases, Prague, Czechoslovakia.
D000818:Animals; D002499:Centrifugation, Density Gradient; D011508:Chondroitin Sulfate Proteoglycans / Q000302:isolation & purification; D002845:Chromatography / Q000379:methods; D006023:Glycoproteins / Q000302:isolation & purification; D019812:Heparan Sulfate Proteoglycans; D006497:Heparan Sulfate / Q000302:isolation & purification; D007553:Isotope Labeling; D008168:Lung / Q000378:metabolism*; D008297:Male; D008970:Molecular Weight; D017830:Octoxynol; D011092:Polyethylene Glycols / Q000302:isolation & purification; D011509:Proteoglycans / Q000302:isolation & purification*; D051381:Rats; D013431:Sulfates / Q000302:isolation & purification
D011508:Chondroitin Sulfate Proteoglycans; D006023:Glycoproteins; D019812:Heparan Sulfate Proteoglycans; D006497:Heparan Sulfate; D017830:Octoxynol; D011092:Polyethylene Glycols; D011509:Proteoglycans; D013431:Sulfates