Phosphatidylcholine synthesis in platelets is stimulated by diacylglycerol but not by phorbol ester.
The biosynthesis of phosphatidylcholine (PC) in platelets was followed by measuring the incorporation of 32Pi. Incorporation into PC was stimulated by treatment with Clostridium perfringens phospholipase C or with the synthetic diacylglycerol sn-1,2-dioctanoylglycerol. However, neither the phorbol ester tumour promoter 12-O-tetradecanoylphorbol-13-acetate or thrombin stimulated 32Pi incorporation into PC. We conclude that phorbol ester does not stimulate the hydrolysis of PC to diacylglycerol in platelets.
Muir
J G
JG
School of Biological Sciences, Flinders University, Bedford Park, South Australia.
Murray
A W
AW
D001792:Blood Platelets / Q000378:metabolism*; D004075:Diglycerides / Q000494:pharmacology*; D005989:Glycerides / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D010703:Phorbol Esters / Q000494:pharmacology*; D010713:Phosphatidylcholines / Q000096:biosynthesis*; D010743:Phospholipids / Q000096:biosynthesis; D013268:Stimulation, Chemical; D013917:Thrombin / Q000494:pharmacology
C011439:1,2-diacylglycerol; D004075:Diglycerides; D005989:Glycerides; D010703:Phorbol Esters; D010713:Phosphatidylcholines; D010743:Phospholipids; D013917:Thrombin