Histoautoradiographic detection of 3H-thymidine and 3H-proline in the vascular wall of domestic swine in experimental atherosclerosis. Is there an effect of saturated/unsaturated fatty acids in high cholesterol diet?
In vitro labeling of arterial wall samples from domestic swine with tritiated thymidine or proline was used to establish the effect of unsaturated (sunflower oil) or saturated (beef tallow) fatty acids in the presence of enteral cholesterol on DNA and collagen synthesis. Histoautoradiography of samples incubated in vitro gives labeling patterns and indices comparable to in vivo labeling. Preliminary results show that these nutrient fats do not differ in their effect on the DNA and collagen synthesis of vascular wall cells both in the presence and absence of intimal changes.
Trimper
B
B
Karl Marx University Leipzig, Department of Medicine, G.D.R.
Massmann
J
J
Bach
G
G
Hecht
A
A
Gebhardt
G
G
D000818:Animals; D001158:Arteries / Q000032:analysis / Q000187:drug effects / Q000378:metabolism*; D001161:Arteriosclerosis / Q000378:metabolism* / Q000473:pathology; D001345:Autoradiography; D002791:Cholesterol, Dietary / Q000008:administration & dosage / Q000494:pharmacology*; D003094:Collagen / Q000096:biosynthesis*; D004247:DNA / Q000096:biosynthesis*; D004036:Diet, Atherogenic; D005223:Fats / Q000008:administration & dosage / Q000494:pharmacology; D005227:Fatty Acids / Q000008:administration & dosage / Q000494:pharmacology; D005231:Fatty Acids, Unsaturated / Q000008:administration & dosage / Q000494:pharmacology; D005260:Female; D010938:Plant Oils / Q000008:administration & dosage / Q000494:pharmacology; D011392:Proline / Q000032:analysis; D000074242:Sunflower Oil; D013552:Swine; D013936:Thymidine / Q000032:analysis
D002791:Cholesterol, Dietary; D005223:Fats; D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D010938:Plant Oils; D000074242:Sunflower Oil; D003094:Collagen; D004247:DNA; C013698:tallow; D011392:Proline; D013936:Thymidine