Turnover products of the apo very low density lipoprotein II messenger RNA from chicken liver.
The mature apo Very Low Density Lipoprotein II (apo VLDLII) mRNA appears in chicken liver within a few hours after estrogen administration. Apart from this mRNA species, shorter RNA molecules hybridizing to apo VLDLII sequences have been detected in rooster liver upon estrogen stimulation. These molecules are present in the non-polyadenylated fraction of the total cellular- and polysomal RNA. Northern blotting and electron microscopy of R-loops were employed to show that these shorter RNA molecules are truncated at their 3'-end. The 3'-termini were further characterized by nuclease S1 analyses, and are located predominantly in the 3' untranslated region of the mRNA. Using a secondary structure model (Shelness and Williams, J. Biol. Chem. 260, 8637-8646, 1985), we show that the 3' termini map mainly in unpaired regions of the structure.
Bakker
O
O
Department of Biochemistry, Groningen University, The Netherlands.
Arnberg
A C
AC
Noteborn
M H
MH
Winter
A J
AJ
Ab
G
G
D000818:Animals; D001053:Apolipoproteins / Q000235:genetics*; D001483:Base Sequence; D002645:Chickens; D004958:Estradiol / Q000494:pharmacology; D005796:Genes; D008079:Lipoproteins, VLDL / Q000235:genetics*; D008099:Liver / Q000378:metabolism*; D008297:Male; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics* / Q000648:ultrastructure
D001053:Apolipoproteins; D008079:Lipoproteins, VLDL; D012333:RNA, Messenger; C027610:apolipoprotein VLDL II; D004958:Estradiol