title: Local abnormalities of coagulation and fibrinolysis and alveolar fibrin deposition in sheep with oleic acid-induced lung injury.
abstract: Extravascular, primarily intra-alveolar, fibrin deposition is a histologic hallmark of acute lung injury in humans and experimental animals, but the mechanisms leading to this finding are poorly understood. To determine whether local abnormalities in the fibrinolytic-procoagulant balance contribute to alveolar fibrin deposition in acute lung injury, we studied bronchoalveolar lavage (BAL) fluids of anesthetized sheep that received intravenous oleic acid. Prominent alveolar fibrin deposition was observed within 2 h after oleic acid-induced lung injury. Procoagulant and fibrinolytic activities were determined in BAL samples of anesthetized, mechanically ventilated sheep before and 2 h after intravenous oleic acid or saline. BAL procoagulant activity was found to be due mainly to tissue factor associated with Factor VII. In baseline BAL samples, we found relatively low levels of procoagulant activity and relatively high levels of fibrinolytic activity. After induction of oleic acid-induced lung injury, the procoagulant activity of BAL was markedly increased, whereas fibrinolytic activity was either depressed or undetectable. Antiplasmin activity was detectable in BAL of sheep after oleic acid-induced lung injury, which contributed at least in part to the depressed fibrinolytic activity observed. These perturbations occurred with the appearance of extensive alveolar fibrin deposition. In control sheep, BAL fibrinolytic activity was decreased, and antiplasmin activity increased modestly after 2 h of mechanical ventilation, but procoagulant activity was unchanged and alveolar fibrin was not observed. Procoagulant activity in lung lymph and plasma after lung injury did not differ from baseline values, and fibrinolytic activity was undetectable in lymph or plasma samples. These data indicate that increased procoagulant activity and concurrent disruption of the balance of coagulation and fibrinolysis establish local conditions that promote acute fibrin deposition in the alveoli of mechanically ventilated, oleic acid-injured sheep.
authors.affiliation: Department of Medicine, University of Texas Health Center, Tyler 75710.
authors.forename: S
authors.initials: S
authors.lastname: Idell
authors.forename: B T
authors.initials: BT
authors.lastname: Peterson
authors.forename: K K
authors.initials: KK
authors.lastname: Gonzalez
authors.forename: L D
authors.initials: LD
authors.lastname: Gray
authors.forename: R
authors.initials: R
authors.lastname: Bach
authors.forename: J
authors.initials: J
authors.lastname: McLarty
authors.forename: D S
authors.initials: DS
authors.lastname: Fair
mesh_terms: D000818:Animals; D000933:Antifibrinolytic Agents / Q000032:analysis / Q000502:physiology; D001777:Blood Coagulation; D001798:Blood Proteins / Q000032:analysis; D001992:Bronchoalveolar Lavage Fluid / Q000032:analysis / Q000473:pathology / Q000503:physiopathology; D005337:Fibrin / Q000378:metabolism*; D005342:Fibrinolysis; D006439:Hemodynamics; D008168:Lung / Q000473:pathology; D008171:Lung Diseases / Q000139:chemically induced / Q000378:metabolism* / Q000503:physiopathology; D008196:Lymph / Q000032:analysis / Q000166:cytology; D019301:Oleic Acid; D009829:Oleic Acids; D011506:Proteins / Q000032:analysis; D011650:Pulmonary Alveoli / Q000378:metabolism*; D012756:Sheep
chemical_list: D000933:Antifibrinolytic Agents; D001798:Blood Proteins; D009829:Oleic Acids; D011506:Proteins; D019301:Oleic Acid; D005337:Fibrin