Identification of [hydroxyproline3]-bradykinin released from human plasma and plasma protein Cohn's fraction IV-4 by trypsin.
Aside from bradykinin (BK), a novel kinin, [Hydroxyproline3]-bradykinin ( [Hyp3]-BK), was isolated from the reaction mixture of human plasma and plasma protein Cohn's fraction IV-4 with trypsin. The liberated kinins were isolated based on procedures which we previously described for the isolation of [Hyp3]-lysyl-bradykinin ( [Hyp3]-Lys-BK) formed by kallikrein. The ratio of the amounts of two kinins thus formed from human plasma protein Cohn's fraction IV-4 were [Hyp3]-BK 25 +/- 4% and BK 75 +/- 4%, similarly to that of [Hyp3]-Lys-BK and Lys-BK, formed by kallikrein, but it varied by persons. The isolation of [Hyp3]-BK and [Hyp3]-Lys-BK suggests that a novel kininogen containing hydroxyproline in the third position of the bradykinin sequence in human plasma protein, possibly undergone post-translational modifications.
Sasaguri
M
M
Department of Internal Medicine, Fukuoka University, School of Medicine, Japan.
Ikeda
M
M
Ideishi
M
M
Arakawa
K
K
D000596:Amino Acids / Q000032:analysis; D001920:Bradykinin / Q000031:analogs & derivatives* / Q000097:blood; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D006909:Hydroxyproline / Q000097:blood*; D014357:Trypsin / Q000494:pharmacology
D000596:Amino Acids; C021698:bradykinin, hydroxy-Pro(3)-; D014357:Trypsin; D006909:Hydroxyproline; D001920:Bradykinin