title: Intact teratogenic immunoglobulins may reach the rat embryo.
abstract: It is known that heterologous antiserum against rat kidney homogenate may induce congenital malformations when injected into pregnant rats during the period of organogenesis. Teratogenic rabbit antibodies against a purified rat renal tubular glycoprotein were isolated, labelled with 125I and injected into pregnant rats on the 10th day of gestation. Extracts of visceral yolk-sacs (VYS) and embryos were obtained 16 h later and chromatographed separately on a Sephacryl S-300 gel filtration column. The resultant chromatograms showed several radioactive peaks, one of which coincided with the eluate of intact rabbit immunoglobulins G (IgG). We interpret the result as an indication that some undigested intact teratogenic IgG were present in VYS and the embryo.
authors.affiliation: Department of Anatomy, University of Medicine and Dentistry of New Jersey, New Jersey Medical School, Newark 07103.
authors.forename: C C
authors.initials: CC
authors.lastname: Leung
authors.forename: B
authors.initials: B
authors.lastname: Cheewatrakoolpong
mesh_terms: D000818:Animals; D000013:Congenital Abnormalities / Q000276:immunology*; D004622:Embryo, Mammalian / Q000276:immunology*; D005260:Female; D005865:Gestational Age; D006023:Glycoproteins / Q000276:immunology*; D007074:Immunoglobulin G / Q000032:analysis*; D007457:Iodine Radioisotopes; D007687:Kidney Tubules, Proximal / Q000032:analysis; D008297:Male; D008431:Maternal-Fetal Exchange; D011247:Pregnancy; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D006023:Glycoproteins; D007074:Immunoglobulin G; D007457:Iodine Radioisotopes