[Comparison of the action of pristan and Freund's incomplete adjuvant on the development of ascites in mice--the recipients of hybridoma cells].
Production of immunodiagnostic preparations based on monoclonal antibodies requires large amounts of such antibodies. Most frequently preparative quantities of monoclonal antibodies are provided by ascitic fluid from hybridoma-carrying mice. Prior to intraperitoneal administration of hybridoma cells mice are subjected to stimulation with pristan, a mineral oil component. The authors showed that the Freund's incomplete adjuvant (FIA) may be as well used for this purpose. The effect of pristan and the FIA on ascites development in mice with hybridomas producing monoclonal antibodies to the Ia-like antigens of man was studied. The stimulants were administered in amounts of 0.5 ml per a mouse. It was shown that irrespective of the stimulant injection time and the number of the administered hybridoma cells the amount of the ascitic fluid formed in female mice BALB/c stimulated with the FIA was 1.5-3 times higher as that in the animals stimulated with Pristane, the antibody titer in the ascitic fluid being unchanged.
Novikov
V V
VV
Trofimova
M N
MN
Zhil'tsova
M A
MA
Sandova
O M
OM
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000096:biosynthesis; D001201:Ascites / Q000209:etiology* / Q000276:immunology; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D005620:Freund's Adjuvant / Q000494:pharmacology*; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D006825:Hybridomas / Q000276:immunology / Q000637:transplantation*; D051379:Mice; D008807:Mice, Inbred BALB C; D008899:Mineral Oil / Q000494:pharmacology*; D013268:Stimulation, Chemical; D013729:Terpenes / Q000494:pharmacology*; D013997:Time Factors
D000911:Antibodies, Monoclonal; D000949:Histocompatibility Antigens Class II; D013729:Terpenes; C009042:pristane; D008899:Mineral Oil; D005620:Freund's Adjuvant
Sravnenie deĭstviia Pristana i nepolnogo ad''iuvanta Freĭnda na formirovanie astsita u mysheĭ--retsipientov gibridomnykh kletok.