Effect of dilution rate and Mg2+ limitation on toxic shock syndrome toxin-1 production by Staphylococcus aureus grown in defined continuous culture.
A toxic shock syndrome isolate of Staphylococcus aureus was grown in a chemostat, in a defined synthetic medium of six amino acids, glucose, two vitamins and salts. Steady states were achieved under limiting and replete Mg2+ conditions and at a range of relative specific growth rates. The biomass and toxic shock syndrome toxin-1 (TSST-1) were estimated at each condition. Under Mg2+ limitation the biomass and TSST-1 production rates were reduced compared to Mg2+ replete conditions. Optimal TSST-1 production occurred at 0.81 relative specific growth rate.
Taylor
D
D
Department of Microbiology, University of Leeds, UK.
Holland
K T
KT
D001427:Bacterial Toxins / Q000096:biosynthesis*; D008274:Magnesium / Q000493:pharmacokinetics*; D012772:Shock, Septic / Q000378:metabolism*; D013211:Staphylococcus aureus / Q000378:metabolism
D001427:Bacterial Toxins; D008274:Magnesium