title: An acrosin inhibitor from boar seminal vesicle fluid immunologically related to the trypsin-kallikrein inhibitor (Kunitz).
abstract: A new acrosin inhibitor was isolated to apparent homogeneity from the fluid of boar seminal vesicles. The inhibitor is immunologically related to the polyvalent trypsin-kallikrein inhibitor from bovine lung known as aprotinin. A crude preparation of the acrosin inhibitor was prepared by immunoaffinity chromatography on anti-aprotinin antibodies bound to Sepharose 4B column. The inhibitor was further purified by affinity chromatography on trypsin immobilized on a Sepharose 4B column, by ion-exchange chromatography on CM-Sephadex C-25, and by reversed-phase high-performance liquid chromatography on a C18 column. The relative molecular mass (Mr) of the inhibitor is about 7,000 as estimated from dodecyl sulfate-polyacrylamide gel electrophoresis. Its amino-acid composition was determined, the sequence of the first 8 amino-acid residues from the N-terminus is Thr-Arg-Asp-Phe-Pro-Pro-Asp-Gly-...
authors.affiliation: Institute of Molecular Genetics, Czechoslovak Academy of Sciences, Prague.
authors.forename: V
authors.initials: V
authors.lastname: Jonáková
authors.forename: D
authors.initials: D
authors.lastname: Cechová
authors.forename: B
authors.initials: B
authors.lastname: Meloun
authors.forename: L
authors.initials: L
authors.lastname: Veselský
mesh_terms: D000176:Acrosin / Q000037:antagonists & inhibitors* / Q000276:immunology; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001826:Body Fluids / Q000032:analysis*; D002417:Cattle; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D008297:Male; D008970:Molecular Weight; D012669:Seminal Vesicles / Q000032:analysis*; D015842:Serine Proteinase Inhibitors; D013552:Swine; D014360:Trypsin Inhibitor, Kunitz Soybean / Q000032:analysis* / Q000276:immunology; D014361:Trypsin Inhibitors / Q000032:analysis*
chemical_list: D000596:Amino Acids; D015842:Serine Proteinase Inhibitors; D014361:Trypsin Inhibitors; D014360:Trypsin Inhibitor, Kunitz Soybean; D000176:Acrosin