The fluorescence quantum yield of vitamin A2.
The fluorescence quantum yield of all-trans 3,4-didehydroretinol (vitamin A2) was measured in hexane at room temperature, using quinine sulfate as a standard. Unlike all-trans retinol (vitamin A1) which possessed a relative quantum yield of 0.0298, 3,4-didehydroretinol was 37 times lower in fluorescence (i.e. 0.0008). In addition, a significant bathochromic shift (both excitation and emission maxima) and a general broadening of the fluorescence spectra were noted for 3,4-didehydroretinol. This information is important not only for the understanding of the basic structure of vitamin A but also the photochemistry of vision.
Tsin
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Division of Life Sciences and Earth and Physical Sciences, University of Texas, San Antonio 78285.
Pedrozo-Fernandez
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Gallas
J M
JM
Chambers
J P
JP
D002851:Chromatography, High Pressure Liquid; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D014801:Vitamin A / Q000031:analogs & derivatives*
C024366:vitamin A2; D014801:Vitamin A