title: Crystallization of actophorin, an actin filament-severing protein from Acanthamoeba.
abstract: Actophorin is an actin monomer-binding and actin filament-severing protein from Acanthamoeba castellanii. It crystallizes out of polyethylene glycol in a form suitable for high resolution x-ray analysis. The crystals are orthorhombic and have the symmetry of the space group P2(1)2(1)2(1) with lattice constants a = 39.8 +/- 0.5, b = 47.3 +/- 0.6, and c = 69.9 +/- 1.6 A. They diffract to a resolution of at least 2.8 A, and the asymmetric unit contains one actophorin monomer of Mr 15,000.
authors.affiliation: Department of Biophysics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
authors.forename: K A
authors.initials: KA
authors.lastname: Magnus
authors.forename: S K
authors.initials: SK
authors.lastname: Maciver
authors.forename: T D
authors.initials: TD
authors.lastname: Pollard
mesh_terms: D000048:Acanthamoeba / Q000378:metabolism*; D000199:Actins / Q000378:metabolism; D000818:Animals; D003460:Crystallization; D008840:Microfilament Proteins / Q000302:isolation & purification* / Q000378:metabolism; D015800:Protozoan Proteins; D014961:X-Ray Diffraction
chemical_list: D000199:Actins; D008840:Microfilament Proteins; D015800:Protozoan Proteins; C047604:actophorin protein, Acanthamoeba