title: Distribution of vasoactive intestinal peptide-sensitive adenylate cyclase activity along the rabbit nephron.
abstract: The effect of vasoactive intestinal peptide (VIP) upon adenylate cyclase (AC) activity has been determined in defined microdissected renal tubules isolated from collagenase-treated rabbit kidneys. In the presence of 10 microM GTP, 1 microM VIP gave marked stimulations of AC over basal values in the bright portion of the distal convoluted tubule (DCTb) (10.1-fold), and in the collecting tubule isolated from the inner stripe of the outer medulla (OMCTi, 7.8-fold). Less pronounced effects of VIP were found in the medullary collecting tubule isolated from the outer stripe (2.5-fold) and in the granular portion of the distal convoluted tubule (2.0-fold). VIP stimulation of AC activity in these segments amounted to 25 to 40% of the effect elicited by other agonists (arginine vasopressin, calcitonin or parathyroid hormone) in their respective target segments. A low response to VIP was observed in the cortical thick ascending limb (1.8-fold) which represented less than 5% of the calcitonin-stimulated AC activity. In the thin descending limb VIP produced a slight and variable stimulation of AC. VIP was without effect upon AC in the convoluted and straight portions of the proximal tubule, the medullary thick ascending limb and the cortical collecting tubule. Half-maximal stimulation of AC by VIP was observed at 26 +/- 10 nM (n = 3) in OMCTi and at 19 nM (n = 2) in DCTb. Related peptides glucagon, secretin and PHI gave lower stimulations of AC compared to VIP in OMCTi. Conversely for rat OMCTi, under identical conditions, glucagon was much more effective than VIP.
authors.affiliation: Department of Physiological Sciences, Medical School, University of Newcastle upon Tyne, Great Britain.
authors.forename: N M
authors.initials: NM
authors.lastname: Griffiths
authors.forename: D
authors.initials: D
authors.lastname: Chabardès
authors.forename: M
authors.initials: M
authors.lastname: Imbert-Teboul
authors.forename: S
authors.initials: S
authors.lastname: Siaume-Perez
authors.forename: F
authors.initials: F
authors.lastname: Morel
authors.forename: N L
authors.initials: NL
authors.lastname: Simmons
mesh_terms: D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D007684:Kidney Tubules / Q000187:drug effects / Q000201:enzymology*; D007685:Kidney Tubules, Collecting / Q000187:drug effects / Q000201:enzymology; D007686:Kidney Tubules, Distal / Q000187:drug effects / Q000201:enzymology; D007687:Kidney Tubules, Proximal / Q000187:drug effects / Q000201:enzymology; D008297:Male; D011817:Rabbits; D014660:Vasoactive Intestinal Peptide / Q000494:pharmacology*
chemical_list: D014660:Vasoactive Intestinal Peptide; D000262:Adenylyl Cyclases