Cholesterol increase in mitochondria: a new method of cholesterol incorporation.
In this study, we report a new method for cholesterol incorporation in mitochondria. This method is based on the coupling of cholesterol to bovine serum albumin (BSA) to form a structure with a spherical shape (designated C-BSA-C). The results show that C-BSA-C induces a specific cholesterol incorporation in mitochondria without interference from BSA. The cholesterol increase is complete in 1 minute, and is dose-related and pH-dependent. Temperature also has an effect. In mitochondria from dog adrenal glands, pregnenolone biosynthesis induced by C-BSA-C is increased, indicating that only cholesterol is transferred to the membrane.
Martínez
F
F
Departamento de Bioquímica, Facultad de Medicina, Universidad Nacional Autónoma de México, D.F.
Eschegoyen
S
S
Briones
R
R
Cuellar
A
A
D000818:Animals; D002784:Cholesterol / Q000378:metabolism*; D004285:Dogs; D004591:Electrophoresis, Polyacrylamide Gel; D008854:Microscopy, Electron; D008928:Mitochondria / Q000378:metabolism*; D008930:Mitochondria, Liver / Q000378:metabolism*; D010758:Phosphorus / Q000032:analysis; D011284:Pregnenolone / Q000096:biosynthesis; D051381:Rats
D010758:Phosphorus; D011284:Pregnenolone; D002784:Cholesterol