title: Reversed-phase preparative chromatography of [D-Ala1]-peptide T amide.
abstract: [D-Ala1]peptide T amide is a metabolically stable and more potent analogue of peptide T, a proposed inhibitor of human immunodeficiency viral infectivity of human T-cell lymphocytes. The peptide was synthesized by solid-phase methods to provide amounts of several grams. The product was purified by chromatography on a 25 cm x 2 in. column of DuPont Zorbax Pro-10 C8 (10 micron) packing. Sample loads of 100-450 mg were chromatographed isocratically in 0.1% trifluoroacetic acid and 5% acetonitrile at a flow-rate of 110 ml/min. Under these conditions, the pure peptide fraction was eluted reproducibly between 15 and 22 min. After solvent removal and lyophilization, the recovery of pure peptide was 50% by weight.
authors.affiliation: Peptide Technologies Corporation, Trinity College, Washington, DC 20017.
authors.forename: M
authors.initials: M
authors.lastname: Knight
authors.forename: G D
authors.initials: GD
authors.lastname: Mack
authors.forename: R
authors.initials: R
authors.lastname: Perkins
authors.forename: T R
authors.initials: TR
authors.lastname: Burke
mesh_terms: D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D009842:Oligopeptides / Q000302:isolation & purification*; D015717:Peptide T
chemical_list: D009842:Oligopeptides; D015717:Peptide T; C055175:peptide T amide