Simultaneous quantitative analysis of methamphetamine and 4-hydroxymethamphetamine in body fluids by gas chromatography/mass spectrometry.
Pentodeuterated 4-hydroxymethamphetamine (HMAMP-d5), 1-(4-hydroxyphenyl)-2-(methyl-d3-amino)-propane-1,2-d2 of high quality was prepared and proved to be a most useful internal standard for quantitative analyses of 4-hydroxymethamphetamine (HMAMP), a main metabolite of methamphetamine (MAMP). Highly reliable results were obtained by gas chromatography/mass spectrometry. Simultaneously we determined also MAMP, amphetamine (AMP), 4-hydroxyamphetamine (HAMP), and HMAMP in body fluids, using two internal standards, HMAMP-d5 for HMAMP and HAMP and pentodeuterated methamphetamine, 1-phenyl-2-(methyl-d3-amino)-propane-1,2-d2, for MAMP and AMP.
Hara
K
K
Department of Legal Medicine, School of Medicine, Fukuoka University, Japan.
Kageura
M
M
Hieda
Y
Y
Kashimura
S
S
D000661:Amphetamine / Q000032:analysis; D000662:Amphetamines / Q000032:analysis; D001826:Body Fluids / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D008694:Methamphetamine / Q000031:analogs & derivatives* / Q000032:analysis*
D000662:Amphetamines; D008694:Methamphetamine; C005368:N-hydroxyamphetamine; C005877:pholedrine; D000661:Amphetamine