title: Na+-sugar cotransport system as a polarization marker during organization of epithelial membrane.
abstract: The present study analyzed the changes in Na+-dependent sugar transport and transepithelial electrical resistance as LLC-PK1 cells reorganize into epithelial membranes. Sugar influx increased to reach a maximum 9 h after plating. The increase in the transepithelial electrical resistance, however, showed a significant delay, reaching steady state 15 h after plating. No changes in the electrochemical Na+ gradient were observed during the reorganization of the epithelial membranes. Kinetic analysis and [3H]phlorizin-binding studies showed that the increase in sugar influx resulted from an increase in the number of carriers. Unidirectional sugar influx measurements indicated that the sugar transporters were primarily located at the apical surface of the epithelial cells. These observations are consistent with the hypothesis that the sorting of native proteins occurs intracellularly before their insertion in the apical membrane, or as an alternative that they are randomly inserted, but then immediately sorted such as any carrier could be detected in the basolateral side during the reorganization process. In addition, the results suggest that the functional development of the apical membrane may occur before the complete sealing of the intercellular space during the development of the occluding junctions. Furthermore, development of the sugar transport system and occluding junctions was inhibited by cycloheximide and puromycin but not by actinomycin D, suggesting that the expression of epithelial cell polarization is probably a posttranslational event in the protein synthesis.
authors.affiliation: Department of Radiology, Massachusetts General Hospital and Harvard Medical School, Boston 02114.
authors.forename: C
authors.initials: C
authors.lastname: Lasheras
authors.forename: J A
authors.initials: JA
authors.lastname: Scott
authors.forename: C A
authors.initials: CA
authors.lastname: Rabito
mesh_terms: D000818:Animals; D001692:Biological Transport; D002460:Cell Line; D002462:Cell Membrane / Q000502:physiology*; D004847:Epithelial Cells; D004848:Epithelium / Q000502:physiology; D008564:Membrane Potentials; D009004:Monosaccharide Transport Proteins / Q000378:metabolism*; D010695:Phlorhizin / Q000378:metabolism
chemical_list: D009004:Monosaccharide Transport Proteins; D010695:Phlorhizin