Myelodysplastic syndrome with Philadelphia-like chromosome without bcr rearrangement.
The case of a 62-year-old Japanese male with a myelodysplastic syndrome and a Philadelphia (Ph)-like chromosome, which probably involved bands 11q23 and 22q11, is presented. Cytogenetic analysis of bone marrow cells revealed a Ph chromosome as well as -5, -7, +8, +11, -16, and an extra Ph. Some of the cells had a normal karyotype. Molecular analysis using breakpoint cluster region probes (5' bcr and 3' bcr) did not detect a rearrangement within the bcr DNA sequences, indicating that the breakpoint at 22q11 occurred outside the bcr. Furthermore, the bone marrow cells from this patient did not express an 8.5-kb c-abl mRNA. Thus, the Ph chromosome in this case differs from that of Ph-positive chronic myelogenous leukemia, and the present case suggests that we should retain the term of "Ph-like chromosome" in such cases.
Ohyashiki
J H
JH
Department of Internal Medicine, Tokyo Medical College, Japan.
Ohyashiki
K
K
Fujieda
H
H
Iwabuchi
H
H
Enomoto
Y
Y
Watanabe
Y
Y
Toyama
K
K
D000754:Anemia, Refractory, with Excess of Blasts / Q000235:genetics*; D015139:Blotting, Southern; D015342:DNA Probes; D005819:Genetic Markers; D006801:Humans; D007621:Karyotyping; D008297:Male; D008875:Middle Aged; D005810:Multigene Family; D009693:Nucleic Acid Hybridization; D010677:Philadelphia Chromosome
D015342:DNA Probes; D005819:Genetic Markers