title: Undecaprenyl diphosphate synthase from Micrococcus luteus B-P 26: essential factors for the enzymatic activity.
abstract: An undecaprenyl diphosphate synthase fraction, which was free of other prenyltransferases and was active without the addition of detergent or phospholipid, was obtained by Sephadex G-100 chromatography of cell-free extracts of Micrococcus luteus B-P 26 cells. The addition of small amounts of Triton X-100 to this fraction caused a marked loss of the enzyme activity, but the activity was gradually restored as further detergent was added. When the enzyme fraction was chromatographed on DEAE-cellulose, the synthase was partially purified, but the activity was not detected unless assayed with addition of the detergent or a lipid fraction of this bacterium. Among the three phospholipids isolated from this bacterium, cardiolipin and phosphatidylglycerol had a marked effect in activating lipid-depleted undecaprenyl diphosphate synthase, but O-lysylphosphatidylglycerol, which occurs prominently in this bacterium, had little effect.
authors.affiliation: Chemical Research Institute of Non-Aqueous Solutions, Tohoku University, Miyagi.
authors.forename: T
authors.initials: T
authors.lastname: Koyama
authors.forename: I
authors.initials: I
authors.lastname: Yoshida
authors.forename: K
authors.initials: K
authors.lastname: Ogura
mesh_terms: D019883:Alkyl and Aryl Transferases; D002845:Chromatography; D003902:Detergents; D004789:Enzyme Activation / Q000187:drug effects; D008837:Micrococcus / Q000201:enzymology*; D010743:Phospholipids / Q000302:isolation & purification / Q000494:pharmacology; D014166:Transferases / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D003902:Detergents; D010743:Phospholipids; D014166:Transferases; D019883:Alkyl and Aryl Transferases; C023266:undecaprenyl pyrophosphate synthetase