Immunoaffinity purification of human erythrocyte prolidase.
A procedure including immunoaffinity gel chromatography of an immobilized monoclonal antibody was used to isolate human erythrocyte prolidase (EC 3.4.13.9). The monoclonal antibody was developed against liver prolidase and the antibody recognized the erythrocyte enzyme. The purification procedure included three steps of DEAE cellulose (batcher), immunoaffinity gel chromatography and gel filtration column chromatography. The overall recovery was approximately 20% and the specific activity of the purified preparation was approximately 260 U/mg of protein, a value exceeding that obtained using conventional procedures.
Endo
F
F
Dept. of Pediatrics, Kumamoto University Medical School, Japan.
Tanoue
A
A
Ogata
T
T
Motohara
K
K
Matsuda
I
I
D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D004150:Dipeptidases / Q000097:blood*; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000201:enzymology*; D006801:Humans
D004150:Dipeptidases; C022449:proline dipeptidase