An ELISA method for the rapid and simple determination of aflatoxin in human serum.
A rapid, simple method for the determination of aflatoxin in human serum using anti-aflatoxin antisera in an indirect, double antibody microtitration plate enzyme-linked immunosorbent assay (ELISA) is described. Direct assay of serum necessitated the availability of a large sample volume, due to the presence of 'non-specific' interference. Addition of methanol to sera removed the interference enabling the analysis to be performed on small volumes (0.5 ml) of serum. The sensitivity of the assay is 20 pg ml-1. Total assay time, including sample preparation, is 4.5 h. No aflatoxin was found in any of the UK samples analysed.
Wilkinson
A P
AP
AFRC Institute of Food Research, Norwich Laboratory, UK.
Denning
D W
DW
Morgan
M R
MR
D000348:Aflatoxins / Q000097:blood*; D011232:Chemical Precipitation; D004797:Enzyme-Linked Immunosorbent Assay; D006358:Hot Temperature; D006801:Humans; D000432:Methanol; D008832:Microchemistry
D000348:Aflatoxins; D000432:Methanol