A sensitive enzyme-linked immunosorbent assay for detection of T-2 toxin with monoclonal antibodies.
Six monoclonal antibodies (mAbs, T-2.1, 2, 3, 4, 5, 6) which react with a trichothecene mycotoxin, T-2 toxin (T-2), were prepared. All antibodies specifically reacted with T-2 but less (0.5% of T-2) with the metabolites such as HT-2 toxin and 3'-hydroxy-T-2 toxin. Significant but less than 0.02% cross-reactivity was observed with T-2 triol, 3'-hydroxy-HT-2 toxin and neosolaniol. No significant reaction with other trichothecenes such as deoxynivalenol, nivalenol, fusarenon-X, crotocin, or roridin A was observed. The least detectable amount of T-2 with the best mAb T-2.1 was 2.5 pg T-2 per assay. This specific and highly sensitive assay for T-2 was applied for the quantitation of T-2 in wheat flour spiked with mycotoxin, with combination of a simple extraction procedure.
Chiba
J
J
Department of Pathology, National Institute of Health, Tokyo, Japan.
Kawamura
O
O
Kajii
H
H
Ohtani
K
K
Nagayama
S
S
Ueno
Y
Y
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006825:Hybridomas / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D012717:Sesquiterpenes / Q000032:analysis*; D013223:Statistics as Topic; D013605:T-2 Toxin / Q000032:analysis* / Q000276:immunology; D014908:Triticum / Q000032:analysis
D000911:Antibodies, Monoclonal; D012717:Sesquiterpenes; D013605:T-2 Toxin