Effect of an anti-ulcer drug, plaunotol, and its metabolites on NAD+ dependent 15-hydroxyprostaglandin dehydrogenase from gastric mucosa.
15-Hydroxyprostaglandin dehydrogenase was partially purified from hog gastric mucosa by about 1000-fold with a 13.5% yield. Its molecular weight was estimated to be 32,000 daltons by gel filtration. The enzyme was inhibited by some metabolites of plaunotol [(2E, 6Z, 10E)-7-hydroxymethyl-3,11,15-trimethyl-2,6,10,14-hexadecatetrae n-1- ol], a new anti-ulcer drug. The inhibition patterns for substrates, prostaglandin E1 and NAD+ were both uncompetitive with Ki values of 7.8 and 19.7 microM, respectively.
Oda
T
T
New Lead Research Laboratories, Sankyo Co., Ltd., Tokyo, Japan.
Ushiyama
S
S
Matsuda
K
K
Iijima
Y
Y
D000818:Animals; D000897:Anti-Ulcer Agents / Q000494:pharmacology*; D004224:Diterpenes; D005233:Fatty Alcohols / Q000494:pharmacology*; D005753:Gastric Mucosa / Q000201:enzymology*; D006911:Hydroxyprostaglandin Dehydrogenases / Q000037:antagonists & inhibitors* / Q000302:isolation & purification; D007700:Kinetics; D013329:Structure-Activity Relationship; D013552:Swine
D000897:Anti-Ulcer Agents; D004224:Diterpenes; D005233:Fatty Alcohols; D006911:Hydroxyprostaglandin Dehydrogenases; C035859:15-hydroxyprostaglandin dehydrogenase; C047686:plaunotol