title: Edge detection in images using Marr-Hildreth filtering techniques.
abstract: Details of the morphology of light microscope images of horseradish peroxidase labeled mammalian neurons in cell culture were investigated. A modified Marr-Hildreth edge-detecting algorithm was used in an image processor to obtain a continuous border of the labeled neurons. The interior of the border was filled to obtain isolated binary silhouettes of the neurons. These silhouettes can be used for further quantitative studies.
authors.affiliation: Laboratory of Neurophysiology, NINCDS, Bethesda, MD 20892.
authors.forename: T G
authors.initials: TG
authors.lastname: Smith
authors.forename: W B
authors.initials: WB
authors.lastname: Marks
authors.forename: G D
authors.initials: GD
authors.lastname: Lange
authors.forename: W H
authors.initials: WH
authors.lastname: Sheriff
authors.forename: E A
authors.initials: EA
authors.lastname: Neale
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D006735:Horseradish Peroxidase; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008433:Mathematics; D008853:Microscopy / Q000379:methods*; D009474:Neurons / Q000166:cytology*
chemical_list: D006735:Horseradish Peroxidase