Enhancement of DMN-induced kidney tumors by 1,2-dichlorovinylcysteine in Swiss-Webster mice.
1,2-Dichlorovinylcysteine (DCVC) is known to cause enlarged nuclei in renal proximal tubule epithelium. This study further characterized the cellular changes induced by DCVC. Also a preliminary investigation of the initiator and promoter potential of DCVC was conducted. When given for 4 weeks in drinking water to Swiss-Webster mice, DCVC (50 or 100 micrograms/ml) caused a progressive change in renal epithelia which was persistent at 23 weeks. Enlarged nuclei with uneven chromatin dispersal, multiple nucleoli, and irregular nuclear membranes resided in enlarged, abnormally-shaped cells. These changes were resolved by week 50. Mice initiated with dimethylnitrosamine developed renal tumors by week 27. Mice fed DCVC (10 or 50 micrograms/ml) for 14 weeks following dimethylnitrosamine initiation, had a slightly higher tumor incidence, a higher incidence of invasive tumors, and had multiple renal tumors unlike animals given dimethylnitrosamine alone.
Meadows
S D
SD
Department of Pharmacology and Toxicology, University of Arizona, Tucson 85724.
Gandolfi
A J
AJ
Nagle
R B
RB
Shively
J W
JW
D000818:Animals; D003043:Cocarcinogenesis; D003505:Cyclodextrins / Q000633:toxicity; D003545:Cysteine / Q000031:analogs & derivatives* / Q000633:toxicity; D004128:Dimethylnitrosamine / Q000633:toxicity*; D004357:Drug Synergism; D007668:Kidney / Q000187:drug effects / Q000473:pathology; D007680:Kidney Neoplasms / Q000139:chemically induced*; D051379:Mice; D047392:beta-Cyclodextrins
D003505:Cyclodextrins; D047392:beta-Cyclodextrins; C039961:S-(1,2-dichlorovinyl)cysteine; C031215:betadex; D003545:Cysteine; D004128:Dimethylnitrosamine