[The effect of SSB-protein from Ehrlich ascites carcinoma cells on activity of various enzymes of DNA replication and repair].
The effect of a single-stranded DNA-binding protein (SSB-protein) form Ehrlich ascites tumour cells (EAT) on the activity of homologous purified DNA-polymerases alpha and beta, DNA-replicase, primase and DNA-polymerases from phage T4 and Bacillus stearothermophillus was studied. It was shown that the SSB-protein caused a 1.5-2.5-fold stimulation of the DNA-polymerase alpha activity on different templates (e.g., denaturated and activated DNA, poly(dA). The degree of stimulation depended on the template type, protein/template ratio and purity of DNA-polymerase alpha. The activity of DNA-polymerase was inhibited by the SSB-protein, when the activated DNA was used as a matrix and was unchanged on the denaturated DNA. The activity of some prokaryotic DNA-polymerases was increased under the influence of the SSB-protein. The protein enhanced the processivity of T4 DNA-polymerase and strongly inhibited the activity of replicase and primase. A conclusion about the complex effect of the SSB-protein on the activity of replicative and repair enzymes is drawn.
Koterov
A N
AN
Novoradovskaia
N A
NA
Filippovich
I V
IV
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000201:enzymology / Q000378:metabolism*; D004256:DNA Polymerase I / Q000037:antagonists & inhibitors / Q000378:metabolism; D004257:DNA Polymerase II / Q000378:metabolism; D004260:DNA Repair; D004261:DNA Replication; D004268:DNA-Binding Proteins / Q000302:isolation & purification / Q000502:physiology*; D004789:Enzyme Activation; D009363:Neoplasm Proteins / Q000502:physiology*; D013604:T-Phages / Q000201:enzymology; D014407:Tumor Cells, Cultured / Q000201:enzymology / Q000378:metabolism
D004268:DNA-Binding Proteins; D009363:Neoplasm Proteins; D004256:DNA Polymerase I; D004257:DNA Polymerase II
Vliianie SSB-belka iz kletok astsitnoĭ kartsinomy Erlikha na aktivnost' nekotorykh fermentov replikatsii i reparatsii DNK.