title: In wheat ctDNA, segments of ribosomal protein genes are dispersed repeats, probably conserved by nonreciprocal recombination.
abstract: Some dispersed repeated sequences and their flanking regions from wheat and maize ctDNAs have been characterized. Two sets of wheat ctDNA repeats were found to be the chloroplast ribosomal protein genes rpl2 and rpl23, plus nonfunctional segments of them, designated rpl2' and rpl23'. Pairwise comparisons were made between the wheat rpl23 and rpl23', and the maize rpl23' sequences. The precise patterns of homology suggest that the divergence of the wheat and maize nonfunctional (rpl23') sequences is being retarded by nonreciprocal recombination, biased by selection for individuals with functional (rpl23) sequences). The implied involvement of these sequences in mechanisms of homologous recombination, and therefore in the creation and spread of new ctDNA variants, is discussed.
authors.affiliation: Institute of Plant Science Research, Cambridge Laboratory, Trumpington, UK.
authors.forename: C M
authors.initials: CM
authors.lastname: Bowman
authors.forename: R F
authors.initials: RF
authors.lastname: Barker
authors.forename: T A
authors.initials: TA
authors.lastname: Dyer
mesh_terms: D001483:Base Sequence; D002736:Chloroplasts; D003062:Codon / Q000235:genetics; D004247:DNA / Q000235:genetics*; D008969:Molecular Sequence Data; D010947:Plants, Toxic; D012091:Repetitive Sequences, Nucleic Acid; D012269:Ribosomal Proteins / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D014026:Nicotiana / Q000235:genetics; D014908:Triticum / Q000235:genetics* / Q000648:ultrastructure; D003313:Zea mays / Q000235:genetics
chemical_list: D003062:Codon; D012269:Ribosomal Proteins; D004247:DNA