Protection from heat-induced protein migration and DNA repair inhibition by cycloheximide.
The mechanism by which Cycloheximide (CHM) protects cells from heat induced killing has been investigated. Cycloheximide (10 micrograms/ml) added for 2 hr before and during a 3 hour heating at 43 degrees C prevented a 40% increase of heat-induced protein accumulation in the nucleus and protected cells (0.0001 vs. 0.15 surviving fraction) from heat-induced killing. Heat-induced DNA repair inhibition was also suppressed when cells were treated with CHM in the above manner. This combination of results suggests that protein accumulation in the nucleus and inhibition of DNA repair are related and these events are associated with CHM protection from heat induced cell killing.
Armour
E P
EP
Department of Radiation Oncology, William Beaumont Hospital, Royal Oak, Michigan 48072.
Lee
Y J
YJ
Corry
P M
PM
Borrelli
M J
MJ
D000818:Animals; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D006224:Cricetinae; D003513:Cycloheximide / Q000494:pharmacology*; D004247:DNA / Q000378:metabolism; D004249:DNA Damage; D004260:DNA Repair / Q000187:drug effects*; D006358:Hot Temperature; D066298:In Vitro Techniques; D014965:X-Rays
D004247:DNA; D003513:Cycloheximide