Characterisation of cis-acting DNA sequences required for the expression of the chicken 5-aminolevulinate synthase gene in Xenopus oocytes.
In this paper, we have constructed deletion and deletion-insertion mutations of the chicken 5-aminolevulinate synthase 5' flanking region and examined the expression of these constructs in microinjected Xenopus oocytes. Utilising this assay, we have delimited the boundary of the 5' flanking region required for expression to be 80 bp upstream from the transcription initiation site. A second major focus of this study has been to define the role of known putative cis-acting sequences in regulating 5-aminolevulinate synthase gene expression. Expression of the insertion-deletion mutants demonstrated that only a TATA box at position -28, and a single GC box at position -78 was necessary for expression of the 5-aminolevulinate synthase gene in Xenopus oocytes. This result is unusual in view of the current state of knowledge of the function of cis-acting sequence elements in transcriptional regulation.
Loveridge
J A
JA
Commonwealth Centre for Gene Technology, Department of Biochemistry, University of Adelaide, Australia.
Borthwick
I A
IA
May
B K
BK
Elliott
W H
WH
D000624:5-Aminolevulinate Synthetase / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D002645:Chickens; D004247:DNA / Q000235:genetics*; D005786:Gene Expression Regulation; D008969:Molecular Sequence Data; D009154:Mutation; D009865:Oocytes / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D014158:Transcription, Genetic; D014170:Transformation, Genetic; D014982:Xenopus laevis
D004247:DNA; D000624:5-Aminolevulinate Synthetase