title: Correlation between sperm creatine phosphokinase activity and sperm concentrations in normospermic and oligospermic men.
abstract: Toward the development of biochemical probes for the assessment of sperm function we have measured the activities of sperm creatine-N-phosphotransferase (CPK). There was a highly significant inverse correlation (P less than 0.001 in all comparisons) between sperm CPK activities and sperm concentrations in specimens of normospermic and oligospermic men with greater than 30 million sperm/ml (0.106 +/- 0.01 SEM, N = 90, expressed as CPK U/100 million sperm), 20-30 million sperm/ml (0.333 +/- 0.07 SEM, N = 30) and 10-20 million sperm/ml (0.583 +/- 0.12 SEM, N = 30) when compared with the CPK values of the less than 10 million/ml specimens (2.242 +/- 0.46 SEM, N = 30). Furthermore, the distribution of CPK activities within these four groups showed that 96%, 67%, 43%, and 4% of the samples, respectively, were in the less than 0.250 CPK U/100 million sperm normal range (mean + 2 SD of the greater than 30 million sperm/ml group). However, there was no relationship between sperm CPK activities and the values of sperm motility (P greater than 0.15) or morphology (P = 0.38) in the samples. The migrated sperm fractions (significantly improved in motility and velocity parameters) showed CPK activities lower than the initial semen specimens (P less than 0.01, N = 150). In fact, in some oligospermic men the CPK activities of the migrated sperm fractions were within the range of normospermic samples.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Obstetrics and Gynecology, Yale University School of Medicine, New Haven, CT 06510.
authors.forename: G
authors.initials: G
authors.lastname: Huszar
authors.forename: M
authors.initials: M
authors.lastname: Corrales
authors.forename: L
authors.initials: L
authors.lastname: Vigue
mesh_terms: D003402:Creatine Kinase / Q000378:metabolism*; D006801:Humans; D008297:Male; D009845:Oligospermia / Q000201:enzymology*; D012661:Semen / Q000201:enzymology; D013076:Sperm Count; D013094:Spermatozoa / Q000201:enzymology*
chemical_list: D003402:Creatine Kinase