[Study of antibody-hapten interaction by measuring velocity of ultrasound propagation].
The patterns of conformational changes induced in pig antibodies to 2,4-dinitrophenyllisin on binding with hapten were investigated by precise measurements of ultrasound velocity. It was shown that upon specific binding with intact antibodies, the observed slow changes of the bulk-clustic properties of the investigated solution reflect self-association of IgG molecules. In the case of interaction of Fab-fragments with hapten no changes of the acoustic properties were found. It was assumed that self-association is related to the increase in the hydrophobicity of Fe-fragments observed upon conformational changes of IgG molecules.
Shil'nikov
G V
GV
Priev
A I
AI
D000818:Animals; D000937:Antigen-Antibody Reactions; D006241:Haptens; D007074:Immunoglobulin G; D007700:Kinetics; D012996:Solutions; D013552:Swine; D014465:Ultrasonics
D006241:Haptens; D007074:Immunoglobulin G; D012996:Solutions
Issledovanie vzaimodeĭstviia antitelo-gapten s pomoshch'iu izmereniia skorosti rasprostraneniia ul'trazvuka.