[Production of immobilized urease adsorbed on polystyrene granules].
We prepared adsorption immobilized Urease (E.C. 3.5.1.5) with a special method of polymer coating of polystyrene granulate as a matrix, and by subsequent adsorption of two different urease products (AWD Dresden, DDR, 3 U/mg, and Serva, Heidelberg, BRD, 41 U/mg). We obtained the strongest activity (4.0 mumol.g-1.min-1) with immobilisates on polystyrene in comparison with polyvinylchloride, and polyurethane layers, if we used AWD urease (PS, PVC, PUR). By using Serva urease we obtained products with a manyfold of activity (12.8 mumol.g.min-1). This high activity enables us to utilize adsorption immobilized urease for the detoxification of biological fluids.
Ernst
B
B
Institut für Klinische Chemie und Laboratoriumsdiagnostik, Bereiches Medizin der Wilhelm-Pieck-Universität Rostock.
D000641:Ammonia / Q000378:metabolism; D004800:Enzymes, Immobilized; D006863:Hydrogen-Ion Concentration; D011137:Polystyrenes; D011140:Polyurethanes; D011143:Polyvinyl Chloride; D014510:Urease / Q000378:metabolism
D004800:Enzymes, Immobilized; D011137:Polystyrenes; D011140:Polyurethanes; D000641:Ammonia; D011143:Polyvinyl Chloride; D014510:Urease
Herstellung adsorptionsimmobilisierter Urease auf Polystyrengranulat.