title: Heterogeneous colony forming ability in vitro of the abnormal clone-derived granulocyte-macrophage precursors in myelodysplastic syndromes.
abstract: The clonal origin of granulocyte-macrophage colony forming cells (CFU-GM) in the myelodysplastic syndrome (MDS) was cytogenetically studied. Chromosome analysis was carried out on single GM-colonies from six patients with MDS, whose bone marrow cells had chromosome abnormalities. Abnormal clone-derived CFU-GM were grown in four patients under the presence of human placental conditioned medium. In the remaining two, all analysed colonies revealed a normal karyotype, although the majority of metaphase cells showed an abnormal karyotype in bone marrow preparations. These results indicate that the abnormal clone-derived CFU-GM in MDS have a clone-by-clone variation in colony forming ability in vitro.
authors.affiliation: Third Department of Internal Medicine, Kyoto Prefectural University of Medicine, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Okuda
authors.forename: S
authors.initials: S
authors.lastname: Yokota
authors.forename: T
authors.initials: T
authors.lastname: Maekawa
authors.forename: Y
authors.initials: Y
authors.lastname: Sonoda
authors.forename: S
authors.initials: S
authors.lastname: Misawa
authors.forename: T
authors.initials: T
authors.lastname: Takino
authors.forename: T
authors.initials: T
authors.lastname: Abe
mesh_terms: D000328:Adult; D000368:Aged; D001853:Bone Marrow / Q000473:pathology; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D005260:Female; D006098:Granulocytes / Q000473:pathology*; D006412:Hematopoietic Stem Cells / Q000473:pathology*; D006801:Humans; D007621:Karyotyping; D008264:Macrophages / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D009190:Myelodysplastic Syndromes / Q000235:genetics / Q000473:pathology*