Influence of readily metabolizable carbon on pentachlorophenol metabolism by a pentachlorophenol-degrading Flavobacterium sp.
The influence of high concentrations of pentachlorophenol (PCP) and readily metabolizable carbon on the activity and viability of a PCP-degrading Flavobacterium sp. was examined in a mineral salts medium. Lags preceding PCP removal by glutamate-grown Flavobacterium cells were greatly attenuated by the addition of glutamate, aspartate, succinate, acetate, glucose, or cellobiose. The effect of these supplementary carbon sources on the apparent lag was not mediated entirely through the stimulation of growth since PCP metabolism accompanied the onset of growth. The specific activity of PCP-degrading cells in the absence of supplementary carbon was 1.51 x 10(-13) +/- 0.08 x 10(-13) g of PCP per cell per h and in the presence of supplementary carbon was 0.92 x 10(-13) +/- 0.09 x 10(-13) g of PCP per cell per h. Glutamate in combination with glucose or cellobiose partially repressed PCP metabolism. PCP removal by PCP-induced, glutamate-grown cells suspended in the presence of 4 g of sodium glutamate per liter was sensitive to shock loads of PCP, with a Ki of about 86.8 micrograms/ml. Subsequent removal rates, however, were more resistant to PCP. Optimal stimulation of PCP removal by sodium glutamate required 3.0 g/liter, about the same concentration as that which saturated growth in the absence of PCP. PCP removal rates decayed within minutes following the transfer of PCP-induced, glutamate-grown cells to media containing PCP without supplementary carbon, and increasing PCP concentrations accelerated the decay.(ABSTRACT TRUNCATED AT 250 WORDS)
Topp
E
E
Gray Freshwater Biological Institute, University of Minnesota, Navarre 55392.
Crawford
R L
RL
Hanson
R S
RS
D001673:Biodegradation, Environmental; D002244:Carbon / Q000378:metabolism*; D002701:Chloramphenicol / Q000494:pharmacology; D002733:Chlorophenols / Q000378:metabolism*; D003470:Culture Media; D005417:Flavobacterium / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D007700:Kinetics; D010416:Pentachlorophenol / Q000378:metabolism*; D012044:Regression Analysis; D012970:Sodium Glutamate / Q000378:metabolism
D002733:Chlorophenols; D003470:Culture Media; D002701:Chloramphenicol; D002244:Carbon; D010416:Pentachlorophenol; D005947:Glucose; D012970:Sodium Glutamate