2,3-Bisphosphoglycerate inhibits hemoglobin synthesis and phosphorylation of initiation factor 2 by casein kinase II in reticulocyte lysates.
2,3-Bisphosphoglycerate inhibited protein synthesis in reticulocyte lysates with 50% inhibition at 2 mM. Glycerate 2,3-P2 increased the Mg2+ optimum for protein synthesis by chelation of Mg2+, but Mg2+ addition did not completely reverse the inhibition, suggesting an additional site of action. eIF-2 has been used to examine the activity of casein kinase II in reticulocyte lysates in response to glycerate 2,3-P2. When glycerate 2,3-P2 was increased to 4mM, phosphorylation of eIF-2 beta was increasingly inhibited. Thus inhibition of phosphorylation of translational components by casein kinase II can be correlated with inhibition of globin synthesis at physiological concentrations of glycerate 2,3-P2.
Gonzatti
M I
MI
Department of Biochemistry, University of California, Riverside 92521.
Traugh
J A
JA
D019794:2,3-Diphosphoglycerate; D000818:Animals; D047388:Casein Kinases; D002474:Cell-Free System; D004163:Diphosphoglyceric Acids / Q000494:pharmacology*; D015852:Eukaryotic Initiation Factor-2; D006454:Hemoglobins / Q000096:biosynthesis*; D066298:In Vitro Techniques; D008274:Magnesium / Q000494:pharmacology; D010448:Peptide Initiation Factors / Q000378:metabolism*; D010766:Phosphorylation; D014176:Protein Biosynthesis / Q000187:drug effects; D047428:Protein Kinase Inhibitors; D011506:Proteins / Q000378:metabolism*; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism*
D004163:Diphosphoglyceric Acids; D015852:Eukaryotic Initiation Factor-2; D006454:Hemoglobins; D010448:Peptide Initiation Factors; D047428:Protein Kinase Inhibitors; D011506:Proteins; D019794:2,3-Diphosphoglycerate; D047388:Casein Kinases; D008274:Magnesium