An 'antigen capture' ELISA for secretory immunoglobulin A antibodies to hepatitis B surface antigen in human saliva.
An enzyme-linked immunosorbent assay (ELISA) was developed which permits the assay of specific secretory immunoglobulin A (sIgA) antibodies against hepatitis B surface antigen (HBsAg) in saliva. The assay is based on the binding of sIgA antibodies present in saliva to microtitre plates coated with excess of F(ab')2 anti-secretory component antibodies, followed by the addition of specific antigen, HBsAg and finally peroxidase-labelled anti-HBsAg. The assay is fast, simple, reproducible and antigen specific as shown by total absence of inhibition of specific antigen by unrelated antigens but significant inhibition of labelled anti-HBsAg by unlabelled anti-HBsAg. The values obtained for hospital personnel exposed to hepatitis infections (0.068 +/- 0.083 U/ml) and for post-icteric hepatitis B patients (0.062 +/- 0.033 U/ml) were significantly higher than values in control subjects (0.013 +/- 0.006 U/ml).
Siddiqi
M A
MA
Department of Immunology, Sher-I-Kashmir Institute of Medical Sciences, Srinagar, India.
Abdullah
S
S
D000328:Adult; D000918:Antibody Specificity; D002846:Chromatography, Affinity; D004797:Enzyme-Linked Immunosorbent Assay / Q000592:standards; D006509:Hepatitis B / Q000175:diagnosis; D006510:Hepatitis B Antibodies / Q000032:analysis* / Q000592:standards; D006514:Hepatitis B Surface Antigens / Q000276:immunology*; D006801:Humans; D007071:Immunoglobulin A, Secretory / Q000032:analysis* / Q000592:standards; D007202:Indicators and Reagents; D012015:Reference Standards; D012463:Saliva / Q000032:analysis* / Q000276:immunology
D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens; D007071:Immunoglobulin A, Secretory; D007202:Indicators and Reagents