[Effect of antibiotics on the secretion and percentage yield of lysozyme from human blood monocytes].
The effect of various doses of benzylpenicillin, streptomycin, cephaloridine (ceporin) and exogenic lysozyme on stability of lysozyme binding to lysosomal membranes of human blood monocytes was studied with the procedure developed by the authors which included estimation of the secreted and total lysozyme and evaluation of the stability index of the lysosomal membranes (SILM). Investigation of the changes in the blood monocyte membrane stability was conducted with constant and short-term (60 minutes) exposure to the antibiotics followed by their through elimination. It was noted all the antibiotics increased the SILM which was evident of labilization of the monocyte lysosomal membranes with respect to lysozyme. However, the mechanism of labilization with respect to various antibiotics was different judging from the amount of the secreted and total lysozyme. Labilization under the effect of benzylpenicillin and streptomycin was most probably due to changes in the lysozyme functional activity in the presence of these antibiotics and that under the effect of exogenic lysozyme was probably due to penetration into the monocytes and simultaneous impairment of the monocyte secretory activity. Cephaloridine had the labilizing effect by inhibition of the lysozyme intracellular synthesis.
Sharonov
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D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D002462:Cell Membrane / Q000187:drug effects / Q000201:enzymology; D002478:Cells, Cultured; D002509:Cephaloridine / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006801:Humans; D009000:Monocytes / Q000187:drug effects* / Q000201:enzymology; D009113:Muramidase / Q000097:blood / Q000378:metabolism*; D010400:Penicillin G / Q000494:pharmacology; D013307:Streptomycin / Q000494:pharmacology; D013997:Time Factors
D000900:Anti-Bacterial Agents; D009113:Muramidase; D002509:Cephaloridine; D010400:Penicillin G; D013307:Streptomycin
Vliianie antibiotikov na sekretsiiu i protsent vykhoda lizotsima iz monotsitov krovi cheloveka.