title: Measurement of specific radioactivity of tryptophan labeled with carbon-14 in plasma by high-performance liquid chromatography with a synchronized accumulating radioisotope detector.
abstract: Measurement of specific radioactivity by a high-performance liquid chromatograph with a synchronized accumulating radioisotope detector was conducted. Accuracy of measurement for an authentic sample containing 0.2 nCi of tryptophan labeled with carbon-14 exceeded 95%. In the case of a plasma sample obtained 120 min following intravenous administration of 15 muCi of labeled tryptophan to a rat, the coefficient of variation was 7.0%.
authors.affiliation: Laboratory of Physical and Pharmaceutical Chemistry, Tokyo College of Pharmacy, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Baba
authors.forename: J
authors.initials: J
authors.lastname: Yamaguchi
authors.forename: M
authors.initials: M
authors.lastname: Horie
mesh_terms: D000818:Animals; D002250:Carbon Radioisotopes; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D051381:Rats; D014364:Tryptophan / Q000097:blood*
chemical_list: D002250:Carbon Radioisotopes; D014364:Tryptophan