In vivo labelling of granulocytes with 99mTc anti-NCA monoclonal antibodies for imaging inflammation.
We looked for inflammatory lesions in 45 patients using in vivo labelled granulocytes. For the purposes of cell labelling we used a murine, monoclonal antibody reacting with NCA and CEA (BW 250/183) (Bosslet et al. 1985) labelled with 99mTc-pertechnetate. All abscesses and other inflammatory lesions were visualized with excellent quality scintigrams between 2 and 6 h after the injection. As the antibody can be stored in a freeze-dried form and labelled at any time in any Department of Nuclear Medicine with 99mTc-pertechnetate, without cell isolation being necessary, the method appears to be suitable even for use in acute diagnosis. No side-effects have so far occurred, even in patients injected up to three times.
Joseph
K
K
Department of Clinical Nuclear Medicine, Philipps University, Marburg, Federal Republic of Germany.
Höffken
H
H
Bosslet
K
K
Schorlemmer
H U
HU
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D015815:Cell Adhesion Molecules; D006023:Glycoproteins / Q000276:immunology*; D006098:Granulocytes; D006801:Humans; D007249:Inflammation / Q000000981:diagnostic imaging*; D007553:Isotope Labeling / Q000379:methods*; D011877:Radionuclide Imaging; D013667:Technetium
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D015815:Cell Adhesion Molecules; D006023:Glycoproteins; D013667:Technetium