A quick and simple method for the quantitation of lactate dehydrogenase release in measurements of cellular cytotoxicity and tumor necrosis factor (TNF) activity.
A simple way of measuring and evaluating lactate dehydrogenase release from lysed tumor cells is described. LDH activity was determined as NADH oxidation or INT reduction over a defined time interval, which was limited by stopping the enzymatic reaction with the inhibitor oxamate. Reaction products were then assayed using a microplate reader. The principle of measuring LDH activity of cellular culture supernatants as a measure of cytotoxicity was successfully applied to a number of murine and human effector-target cell combinations (macrophages, monocytes, NK cells and cytotoxic T cells with P815, A375, K562 and Yac-1 tumor cells) as well as to the determination of TNF activity on L929 cells. Comparison with 51Cr release assays suggests that LDH release assays are an appropriate and possibly preferable means of measuring cellular cytotoxic reactions. This LDH release assay combines the advantages of reliability and simple evaluation characteristic of radioisotope release assays with the convenience of speed and avoidance of radioactivity.
Decker
T
T
Department of Immunobiology, Fraunhofer Institute of Toxicology, Hanover, F.R.G.
Lohmann-Matthes
M L
ML
D000818:Animals; D002460:Cell Line; D003601:Cytotoxicity Tests, Immunologic / Q000379:methods; D005312:Fetal Blood / Q000502:physiology; D006801:Humans; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000032:analysis* / Q000037:antagonists & inhibitors / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009243:NAD; D010084:Oxidation-Reduction; D013778:Tetrazolium Salts; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis*
D013778:Tetrazolium Salts; D014409:Tumor Necrosis Factor-alpha; D009243:NAD; C013574:iodonitrotetrazolium; D007770:L-Lactate Dehydrogenase