title: Effect of dilution rate and Mg2+ limitation on toxic shock syndrome toxin-1 production by Staphylococcus aureus grown in defined continuous culture.
abstract: A toxic shock syndrome isolate of Staphylococcus aureus was grown in a chemostat, in a defined synthetic medium of six amino acids, glucose, two vitamins and salts. Steady states were achieved under limiting and replete Mg2+ conditions and at a range of relative specific growth rates. The biomass and toxic shock syndrome toxin-1 (TSST-1) were estimated at each condition. Under Mg2+ limitation the biomass and TSST-1 production rates were reduced compared to Mg2+ replete conditions. Optimal TSST-1 production occurred at 0.81 relative specific growth rate.
authors.affiliation: Department of Microbiology, University of Leeds, UK.
authors.forename: D
authors.initials: D
authors.lastname: Taylor
authors.forename: K T
authors.initials: KT
authors.lastname: Holland
mesh_terms: D001427:Bacterial Toxins / Q000096:biosynthesis*; D008274:Magnesium / Q000493:pharmacokinetics*; D012772:Shock, Septic / Q000378:metabolism*; D013211:Staphylococcus aureus / Q000378:metabolism
chemical_list: D001427:Bacterial Toxins; D008274:Magnesium