Metal-induced alteration of the cell membrane/cytoplasm complex studied by flow cytometry and detergent lysis.
Flow cytometric analysis of the cell cycle is most effectively accomplished with membrane-/cytoplasm-free ("clean") nuclei. Non-ionic detergents (e.g. NP40 or Triton X-100) commonly are employed to solubilize cell membranes/cytoplasm to produce "clean" nuclei. Treatment of murine erythroleukemic cells (MELC) with tri-n-butyltin methoxide, cadmium acetate, zinc sulfate, or lead acetate alters the properties of the cell membrane/cytoplasm complex making it resistant to NP40 dissolution. On a molar basis, the organotin compound was more effective in inducing resistance to detergent-mediated dissolution than the inorganic metal compounds. Resistance to NP40-mediated dissolution was manifested as an increase in the flow cytometric parameters 90 degrees scatter and fluorescein isothiocyanate (FITC) fluorescence and was confirmed by light microscopy.
Zucker
R M
RM
NSI, Environmental Sciences, Research Triangle Park, NC 27709.
Elstein
K H
KH
Easterling
R E
RE
Massaro
E J
EJ
D000085:Acetates; D000818:Animals; D002104:Cadmium / Q000633:toxicity; D002453:Cell Cycle / Q000187:drug effects; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects*; D002467:Cell Nucleus / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003593:Cytoplasm / Q000187:drug effects*; D003902:Detergents / Q000494:pharmacology; D000431:Ethanol / Q000494:pharmacology; D005434:Flow Cytometry; D008670:Metals / Q000633:toxicity*; D051379:Mice; D009942:Organometallic Compounds / Q000633:toxicity; D013431:Sulfates / Q000633:toxicity; D014220:Trialkyltin Compounds / Q000633:toxicity; D015032:Zinc / Q000633:toxicity; D019287:Zinc Sulfate
D000085:Acetates; D003902:Detergents; D008670:Metals; D009942:Organometallic Compounds; D013431:Sulfates; D014220:Trialkyltin Compounds; D002104:Cadmium; D000431:Ethanol; C011559:tributyltin; D019287:Zinc Sulfate; C028031:cadmium acetate; D015032:Zinc; C008261:lead acetate