Cobinding of bilirubin and laurate to human serum albumin: spectroscopic characterization of stoichiometric complexes.
Light absorption and CD spectra of bound bilirubin and albumin fluorescence spectra have been recorded from mixtures containing albumin, A, bilirubin, B, and laurate, L, in Tris-NaCl buffer at pH 8.2, 25 degrees C. Concentrations of the corresponding stoichiometric complexes, ABiLj, for i = 0/3 and j = 0/3, have been calculated from previously determined stoichiometric cobinding constants (H. Sato et al. (1988) Arch. Biochem. Biophys. 260, 811-821). Spectral data of the complexes have finally been found by iterative computer fitting using the principle of several acceptable solutions (R. Brodersen et al. (1987) Eur. J. Biochem. 169, 487-495). The results were utilized at the microscopic level to investigate ligand-induced conformational changes. When laurate was bound to AB, a decrease of the distance between Trp-214 and the bound bilirubin occurred, as measured according to Förster's principle. The distances were 21.9 +/- 0.3 A in AB, 19.7 +/- 0.3 A in ABL, and 17.9 +/- 0.2 A in ABL2.
Honoré
B
B
Institute of Medical Biochemistry, University of Aarhus, Denmark.
Sato
H
H
Brodersen
R
R
D001663:Bilirubin / Q000378:metabolism*; D002942:Circular Dichroism; D006801:Humans; D066298:In Vitro Techniques; D007850:Lauric Acids / Q000378:metabolism*; D046911:Macromolecular Substances; D011485:Protein Binding; D012709:Serum Albumin / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013057:Spectrum Analysis; D014364:Tryptophan
D007850:Lauric Acids; D046911:Macromolecular Substances; D012709:Serum Albumin; C030358:lauric acid; D014364:Tryptophan; D001663:Bilirubin