[Isolation of highly-purified NADP(H)-dependent enzymes from the rat liver using NADP-hydrazidoadipoyl oxypropyl sepharose].
NADP-hydrazidoadipoyl oxypropyl sepharose was synthesized from epoxyactivated sepharose through a hydrazid derivative and used for isolation of NADP(H)-dependent enzymes such as glutathione reductase, isocitrate dehydrogenase and malate dehydrogenase. The isolation technique involves fractionation with ammonium sulphate, affinity chromatography on NADP-hydrazidoadipoyl oxypropyl sepharose and chromatography on hydroxylapatite. The proposed technique enabled the authors to obtain malate dehydrogenase isocitrate dehydrogenase and glutathione reductase preparations homogeneous according to SDS-electrophoresis in polyacrylamide gel.
Bondar'
V S
VS
Kuznetsov
P V
PV
Mezhevikin
V V
VV
D000818:Animals; D002846:Chromatography, Affinity; D004800:Enzymes, Immobilized / Q000302:isolation & purification; D005980:Glutathione Reductase / Q000302:isolation & purification; D007521:Isocitrate Dehydrogenase / Q000302:isolation & purification; D008099:Liver / Q000201:enzymology*; D008291:Malate Dehydrogenase / Q000302:isolation & purification; D009249:NADP; D051381:Rats; D011919:Rats, Inbred Strains; D012685:Sepharose / Q000031:analogs & derivatives
D004800:Enzymes, Immobilized; D009249:NADP; D012685:Sepharose; D008291:Malate Dehydrogenase; D007521:Isocitrate Dehydrogenase; D005980:Glutathione Reductase
Poluchenie vysokoochishchennykh NADF(H)-zavisimykh fermentov iz pecheni krys s ispol'zovaniem NADF-gidrazidoadipoiloksipropilsefarozy.