Experimental osteoarthritic articular cartilage is enriched in guanidine soluble type VI collagen.
Experimental osteoarthritis was surgically induced in the right knee joint of dogs; the left knee served as a control. Articular cartilage was extracted with 4 M guanidinium chloride, 0.05 M sodium acetate, pH 6.0, containing proteinase inhibitors and the proteins purified by associative CsCl density gradient centrifugation. Equal quantities of protein were electrophoresed in agarose-acrylamide gradient gels and the high molecular weight type VI collagen bands detected in immunoblots with a polyclonal antiserum. Type VI collagen bands between 185 and 220 kDa were evident in the pathological specimens of dogs sacrificed 3, 5, and 7 months after surgery and were either absent or only very weakly visible in the controls. These results demonstrate that experimental osteoarthritic cartilage is enriched in 4 M guanidine-soluble type VI collagen.
McDevitt
C A
CA
Department of Musculoskeletal Research, Cleveland Clinic Foundation Research Institute, Ohio 44195.
Pahl
J A
JA
Ayad
S
S
Miller
R R
RR
Uratsuji
M
M
Andrish
J T
JT
D000818:Animals; D015153:Blotting, Western; D002358:Cartilage, Articular / Q000378:metabolism*; D003094:Collagen / Q000378:metabolism*; D004285:Dogs; D006146:Guanidines; D008970:Molecular Weight; D010003:Osteoarthritis / Q000378:metabolism*; D012995:Solubility
D006146:Guanidines; D003094:Collagen