title: Use of a monoclonal antibody for the therapeutic monitoring of cyclosporine in plasma and whole blood.
abstract: The evaluation of a radioimmunoassay (RIA) for the monitoring of cyclosporine (CSA) that utilizes a monoclonal antibody specific for the parent compound is described. The assay exhibited good analytical recovery (greater than 95%), precision (coefficient of variation less than 10%), and linearity in plasma and whole blood specimens. The percentage cross reactivity of the major CSA metabolites M-17, M-1, and M-21 was less than 2%. The RIA gave values that were on average indistinguishable from those obtained by high-pressure liquid chromatography in plasma and whole blood specimens obtained from 49 renal transplant recipients.
authors.affiliation: Department of Clinical Chemistry, Health Sciences Centre, Winnipeg, Manitoba, Canada.
authors.forename: K R
authors.initials: KR
authors.lastname: Copeland
authors.forename: R W
authors.initials: RW
authors.lastname: Yatscoff
mesh_terms: D000911:Antibodies, Monoclonal; D002851:Chromatography, High Pressure Liquid; D003524:Cyclosporins / Q000097:blood*; D006801:Humans; D010949:Plasma / Q000032:analysis*; D011863:Radioimmunoassay; D015203:Reproducibility of Results
chemical_list: D000911:Antibodies, Monoclonal; D003524:Cyclosporins