title: Loss of variable antigen during transformation of Trypanosoma brucei rhodesiense from bloodstream to procyclic forms in the tsetse fly.
abstract: A pleomorphic line of Trypanosoma brucei rhodesiense expressing a single variable antigen was used to quantify the rate of loss of the surface coat from bloodstream forms transforming to procyclics in the tsetse fly, Glossina morsitans, and in in vitro culture. Loss of variable antigen occurred at similar rates in the crop and anterior portion of the midgut of tsetse flies and in in vitro culture, but in the posterior portion of the fly midgut it occurred 2-3 times faster. The posterior portion of the midgut is the most important site for transformation of bloodstream-form trypanosomes to procyclics, and the dynamics of at least one component of this process are therefore not accurately paralleled in vitro.
authors.affiliation: Department of Zoology, University of Glasgow, UK.
authors.forename: C M
authors.initials: CM
authors.lastname: Turner
authors.forename: J D
authors.initials: JD
authors.lastname: Barry
authors.forename: K
authors.initials: K
authors.lastname: Vickerman
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000032:analysis*; D014346:Trypanosoma brucei brucei / Q000254:growth & development / Q000276:immunology* / Q000648:ultrastructure; D014370:Tsetse Flies / Q000469:parasitology*; D014643:Variant Surface Glycoproteins, Trypanosoma / Q000096:biosynthesis
chemical_list: D000953:Antigens, Protozoan; D014643:Variant Surface Glycoproteins, Trypanosoma