Extracellular ATP and ADA-buffer enable chick embryo fibroblasts to grow in secondary cultures in protein-free, hormone-free, extracellular growth factor-free medium.
The replacement of HEPES with ADA buffer and addition of ATP to the serum-free, protein-free Eagle's minimal essential medium (EMEM) caused stimulation of proliferation in sparse, secondary cultures of resting chick embryo fibroblasts. Better cell growth and highly reproducible results were obtained if the cells had been dispersed from primary cultures with EGTA and thereafter remained without any further contact with serum or other extracellular proteins than when trypsin was used. The frequent changes of culture medium caused no retardation of cell growth. The observed more than 12 fold increase in the cell number after stimulation of the cultures with ATP is interpreted in favour of the conceptions assuming a significant role of intracellular calcium and intracellular pH in regulation of cell proliferation.
Pietrzkowski
Z
Z
Department of Cell Biology, Jan Zurzycki Institute of Molecular Biology, Jagellonian University, Kraków, Poland.
Korohoda
W
W
D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000818:Animals; D002021:Buffers; D002453:Cell Cycle / Q000187:drug effects*; D002642:Chick Embryo; D003470:Culture Media; D005347:Fibroblasts / Q000166:cytology; D006133:Growth Substances / Q000494:pharmacology; D014176:Protein Biosynthesis
D002021:Buffers; D003470:Culture Media; D006133:Growth Substances; D000255:Adenosine Triphosphate