Digitization of electron micrographs: a comparison of three different types of scanners.
Some aspects of digitization of electron micrographs have been investigated. The performances of a flat-bed, a rotating drum, and a diode array scanner have been evaluated. Estimates have been achieved for resolution, mechanical and optical stability, and optical density response. It is concluded that for routine transmission electron microscopy of, for example, negatively stained biologic specimens, a diode array scanner produces data good enough to obtain resolutions at a level normally expected. High speed is the major advantage with this type of equipment. However, for high-resolution work it is necessary to use a conventional scanner with a relatively slow scan speed.
Olofsson
A
A
Department of Medical Biophysics, Karolinska Institutet, Stockholm, Sweden.
Kavéus
U
U
Hebert
H
H
D000703:Analog-Digital Conversion; D003720:Densitometry / Q000295:instrumentation; D007091:Image Processing, Computer-Assisted / Q000295:instrumentation*; D008854:Microscopy, Electron