title: Enhanced production of poxvirus vectors by high speed rolling.
abstract: The amount of CPE produced by vaccinia vectors in cultures rolled at 96 revolutions per minute (rpm) was significantly enhanced when compared to cultures rolled at 2 rpm or held stationary. Cultures rolled at 96 rpm had significantly more foci (46.3 +/- 8.4) than cultures held stationary (16.3 +/- 6.5) or rolled at 2 rpm (27.6 +/- 2.8). The size of foci in cultures rolled at 96 rpm was also significantly larger than foci in cultures rolled at 2 rpm or held stationary (F = 12.65, P less than 0.01). Cultures infected with a low multiplicity of infection and rolled at 96 rpm developed maximum CPE 4 days earlier than stationary cultures. When compared to stationary conditions, cultures rolled at 2 rpm had a 7.0-fold geometric mean increase in viral yield. However, cultures rolled at 96 rpm had a 75-fold geometric mean increase in viral yield. These data suggest that cultures inoculated with vaccinia vectors should be rolled to maximize viral replication.
authors.affiliation: Department of Medical Microbiology and Immunology, Ohio State University, Columbus.
authors.forename: J H
authors.initials: JH
authors.lastname: Hughes
authors.forename: J F
authors.initials: JF
authors.lastname: Sheridan
mesh_terms: D003588:Cytopathogenic Effect, Viral; D005822:Genetic Vectors; D014616:Vaccinia virus / Q000235:genetics / Q000502:physiology*; D014776:Virus Cultivation / Q000379:methods*; D014779:Virus Replication