Autocrine differentiation-inhibiting factor (ADIF) from chicken erythroleukemia cells acts on human and mouse early BFU-E erythroid progenitors.
tsAEV-LSCC HD3 chicken erythroid cells transformed by the avian erythroblastosis virus (AEV) secrete an autocrine differentiation-inhibiting factor, ADIF, which blocks differentiation without affecting proliferation of the chicken erythroid cells that synthesize and secrete it into the culture medium. The chicken erythroleukemia cell ADIF activity is not restricted to avians. It prevents dimethylsulfoxide (DMSO) from stimulating murine Friend erythroleukemia cells to synthesize hemoglobin. ADIF also blocks erythroid differentiation in normal human and murine bone marrow where it selectively targets the early BFU-E (burst-forming) erythroid precursor cells without affecting the more advanced CFU-E erythroid precursor cells or cells of the different granulocyte-macrophage lineage.
Krsmanovic
V
V
Laboratoire de Biologie Moléculaire (CNRS, UA 1176), Université Claude Bernard, Lyon, France.
Morardet
N
N
Biquard
J M
JM
Mouchirout
G
G
Fasciotto
B
B
Ristic
A
A
Parmentier
C
C
Blanchet
J P
JP
Kanazir
D
D
Durkin
J P
JP
D000818:Animals; D001853:Bone Marrow / Q000187:drug effects*; D001854:Bone Marrow Cells; D002454:Cell Differentiation / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D002645:Chickens; D004920:Erythropoiesis / Q000187:drug effects*; D006801:Humans; D066298:In Vitro Techniques; D004915:Leukemia, Erythroblastic, Acute / Q000473:pathology*; D051379:Mice; D011506:Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D013045:Species Specificity; D014407:Tumor Cells, Cultured
D011506:Proteins