title: Rapid preparation of the nicotinic acetylcholine receptor for crystallization in detergent solution.
abstract: A novel rapid purification method for the nicotinic acetylcholine receptor from Torpedo electric tissue was developed. It allows preparation of 10 mg quantities of pure and stabile receptor protein within 2 days. This protein is used for crystallization attempts. Conditions are described which reproducibly yield crystals.
authors.affiliation: Institut für Biochemie, Freie Universität Berlin, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Hertling-Jaweed
authors.forename: G
authors.initials: G
authors.lastname: Bandini
authors.forename: A
authors.initials: A
authors.lastname: Müller-Fahrnow
authors.forename: V
authors.initials: V
authors.lastname: Dommes
authors.forename: F
authors.initials: F
authors.lastname: Hucho
mesh_terms: D000818:Animals; D003460:Crystallization; D003902:Detergents; D004557:Electric Organ / Q000378:metabolism; D005960:Glucosides; D011978:Receptors, Nicotinic / Q000302:isolation & purification*; D012996:Solutions; D014101:Torpedo
chemical_list: D003902:Detergents; D005960:Glucosides; D011978:Receptors, Nicotinic; D012996:Solutions; C018619:octyl-beta-D-glucoside