Rapid serotyping of enteropathogenic Yersinia enterocolitica strains by co-agglutination.
A rapid co-agglutination test using monospecific antisera was developed for the serological typing of enteropathogenic strains of Yersinia enterocolitica. A total of 70 bacterial strains (17 reference strains and 53 clinical isolates) were examined. Absorption of immune sera against serotypes O:3, O:8 and O:9 with their heterologous antigens (S-LPS) was necessary to avoid the appearance of different cross-reactions, as observed by co-agglutination. The proteins present in the S-LPS preparations obtained from each serotype seemed to be responsible for such cross-reactions. Results obtained with a total of 57 clinical isolates belonging to other members of the family Enterobacteriaceae indicate a high specificity of the assay.
Fernandez-Lago
L
L
Department of Microbiology, Faculty of Pharmacy, University of Salamanca, Spain.
Rodriguez-Nebreda
M S
MS
Chordi
A
A
D000372:Agglutination Tests / Q000379:methods*; D007106:Immune Sera / Q000276:immunology; D012703:Serotyping / Q000379:methods*; D015008:Yersinia enterocolitica / Q000145:classification* / Q000302:isolation & purification
D007106:Immune Sera