title: Structural organization of the multienzyme complex of mammalian aminoacyl-tRNA synthetases.
abstract: The multienzyme complexes of mammalian aminoacyl-tRNA synthetases were purified from rat liver, rabbit liver, and rabbit reticulocytes according to the procedure slightly modified from Kellermann et al. [Kellermann, O., Brevet, A., Tonetti, H., & Waller, J.-P. (1979) Eur. J. Biochem. 99, 541-550]. Three forms of the synthetase complex with slightly different protein compositions were identified, suggesting a microheterogeneity of the synthetase complex. The hydrodynamic properties and the protein composition of the purified complexes were determined. The electron micrographs of the complex showed mostly amorphous particles and some hollow rings with an outer diameter of 164 A and an inner diameter of 42 A. The predicted hydrodynamic properties of several models of the complex were calculated. The properties of a ring model appear to best fit with those of the synthetase complex.
authors.affiliation: Department of Chemistry, Georgetown University, Washington, D.C. 20057.
authors.forename: D E
authors.initials: DE
authors.lastname: Godar
authors.forename: D E
authors.initials: DE
authors.lastname: Godar
authors.forename: V
authors.initials: V
authors.lastname: Garcia
authors.forename: A
authors.initials: A
authors.lastname: Jacobo
authors.forename: U
authors.initials: U
authors.lastname: Aebi
authors.forename: D C
authors.initials: DC
authors.lastname: Yang
mesh_terms: D000604:Amino Acyl-tRNA Synthetases / Q000302:isolation & purification; D000818:Animals; D008854:Microscopy, Electron; D008958:Models, Molecular; D009097:Multienzyme Complexes / Q000302:isolation & purification; D011487:Protein Conformation; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D009097:Multienzyme Complexes; D000604:Amino Acyl-tRNA Synthetases