Purification and partial biochemical characterization of an edema inducing phospholipase A2 from Vipera russelli (Russell's viper) snake venom.
A phospholipase A2 (VRV PL-VI) from Vipera russelli venom was purified to homogeneity in a single step on CM-Sephadex C-25 column. VRV PL-VI is a basic protein with a mol. wt of about 12,000 and showed a basic pH optimum and a high temperature maximum. It hydrolysed purified phospholipids in the order of phosphatidylethanolamine greater than phosphatidylcholine much greater than phosphatidylserine greater than phosphatidylinositol = 0. It is toxic with an LD50 value (i.p.) of 3.5 micrograms/g body weight in mice and it induced persistent edema in the mouse foot pad.
Vishwanath
B S
BS
Department of Biochemistry, University of Mysore, India.
Kini
R M
RM
Gowda
T V
TV
D000818:Animals; D002850:Chromatography, Gel; D004487:Edema / Q000139:chemically induced*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007928:Lethal Dose 50; D051379:Mice; D008970:Molecular Weight; D010740:Phospholipases / Q000302:isolation & purification*; D010741:Phospholipases A / Q000302:isolation & purification* / Q000633:toxicity; D054467:Phospholipases A2; D014757:Viper Venoms / Q000032:analysis* / Q000633:toxicity
D014757:Viper Venoms; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2