title: Immunological control of hematophagous arthropod vectors: utilization of novel antigens.
abstract: Immunological control of ixodid ticks requires development of a vaccine that stimulates an effective anti-tick response. However, it is important to avoid induction of intense host skin reactivity to tick feeding. Salivary-gland-derived molecules are introduced into the host during ixodid engorgement; therefore, use of these moieties might not be an optimal immunization strategy. Antigens not normally involved in acquired resistance can be used to induce anti-tick immunity. These "novel" antigens, obtained from tick gut absorptive surface, are not introduced into the host during tick feeding, but are exposed to host-immune effector elements in the blood meal, resulting in ixodid rejection, prevention of ova production and death. Anti-tick immunity is induced with microgram quantities of this ixodid gut antigen preparation. Sera obtained from immunized animals were used to identify antibody-reactive components of the resistance-inducing extract. Tick gut absorptive surface antigen glycoconjugates were identified by lectin blotting, using a series of probes with different carbohydrate specificities. The lectins, peanut and wheat germ agglutinin, and immunized host antibodies bound extract components with similar molecular weights. Solubilization and fractionization of tick gut proteins with the non-ionic detergent Triton X-114 is described.
authors.affiliation: Department of Microbiology and Immunology, School of Medicine, University of North Dakota, Grand Fork 58202.
authors.forename: S K
authors.initials: SK
authors.lastname: Wikel
mesh_terms: D000818:Animals; D000917:Antibody Formation; D000941:Antigens / Q000276:immunology*; D001096:Arachnid Vectors / Q000276:immunology*; D005260:Female; D006168:Guinea Pigs; D015151:Immunoblotting; D008297:Male; D013984:Tick Infestations / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D013987:Ticks / Q000276:immunology*
chemical_list: D000941:Antigens