Conversion of dihydrotestosterone to androstanediol glucuronide by female sexual skin.
Sexual skin biopsies from 13 normal women were obtained and minces/3-h studied after adding either [3H]dihydrotestosterone (DHT) or [3H]androstanediol (3 alpha diol) to RPMI-1640 medium in a Dubnoff apparatus. Unconjugated or conjugated androgens (after hydrolysis) were purified by three chromatography steps. Formation of 3 alpha diol and 3 alpha diol glucuronide (3 alpha diolG) was linear with time. The conversion of DHT to DHT17 beta G was only 4.4 +/- 0.5%/200 mg/3 h, while conversion to 3 alpha diol was 32 +/- 1.7%. The back conversion of 3 alpha diol to DHT was 30 +/- 3% and conversion to 3 alpha diolG was 4.5 +/- 1.25%. The product of the conversion separately measured of DHT to 3 alpha diol and 3 alpha diol to 3 alpha diolG was 1.5%, which is not very different than the overall conversion rate of DHT to 3 alpha diolG of 1.4%. This study indicates that the predominant path in this tissue is DHT in equilibrium 3 alpha diol----3 alpha diolG, rather than formation of DHT17 beta G and then 3 alpha reduction to 3 alpha diolG.
Pasupuleti
V
V
Department of Medicine, University of Southern California, Los Angeles 90033.
Lobo
R
R
Horton
R
R
D000328:Adult; D015113:Androstane-3,17-diol / Q000031:analogs & derivatives / Q000032:analysis / Q000096:biosynthesis*; D000732:Androstanols / Q000096:biosynthesis*; D001706:Biopsy; D002845:Chromatography; D002854:Chromatography, Paper; D013196:Dihydrotestosterone / Q000378:metabolism*; D005260:Female; D006801:Humans; D008875:Middle Aged; D012867:Skin / Q000201:enzymology / Q000378:metabolism*; D014621:Vagina / Q000201:enzymology / Q000378:metabolism*
D000732:Androstanols; D013196:Dihydrotestosterone; D015113:Androstane-3,17-diol; C028895:androstane-3,17-diol glucuronide