Karyometric and cytophotometric studies of the oligodendroglia in the corpus callosum of the rat after hypoxia.
Karyometric and cytophotometric investigations were conducted using an automatic analyser "Morphoquant" on oligodendroglial nuclei of corpus callosum following acute or moderate hypoxia in Wistar rats. Results of our studies demonstrated karyometric changes in nuclei of oligodendroglia cells after hypoxia, with deviations distinct in the early and late period after exposure to hypoxia. Cytophotometric analysis of a relative DNA content in cell nuclei of oligodendroglia demonstrated an increased extinction of DNA in the early period and a decreased extinction in the late period after acute or moderate hypoxia. The changes in oligodendroglia may be primarily responsible for the molecular structure lesion in myelin, observed as a result of hypoxia.
Wender
M
M
Department of Neurology, Medical School Poznań, Poland.
Szczech
J
J
Godlewski
A
A
Grochowalska
A
A
D000818:Animals; D002467:Cell Nucleus / Q000648:ultrastructure; D003337:Corpus Callosum / Q000166:cytology* / Q000473:pathology; D004247:DNA / Q000032:analysis*; D005434:Flow Cytometry; D000860:Hypoxia / Q000473:pathology; D007620:Karyometry; D009457:Neuroglia / Q000166:cytology*; D009836:Oligodendroglia / Q000166:cytology*; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values
D004247:DNA