title: Partitioning behaviour in aqueous two-phase systems and fractionation by counter-current distribution of chick-embryo erythrocytes with numerical resolution of distribution curves.
abstract: The partition of chick-embryo and young-chick erythrocytes in dextran-poly-(ethylene glycol) two-phase systems depends on the interfacial tension and electrical potential differences between the phases. Counter-current distribution with charged 5% dextran-poly(ethylene glycol) systems has proved to be an adequate method for the separation of primitive and definitive erythrocytes present in chick embryos when a phase settling time of 20 min is used. The computer-aided numerical resolution of experimental curves has shown the existence of subpopulations which could not have been detected by using conventional methods.
authors.affiliation: Departamento de Bioquímica, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Ferrer
authors.forename: M
authors.initials: M
authors.lastname: Martin
authors.forename: R
authors.initials: R
authors.lastname: Alonso
authors.forename: J
authors.initials: J
authors.lastname: Luque
mesh_terms: D000818:Animals; D002642:Chick Embryo; D002645:Chickens; D003377:Countercurrent Distribution; D004910:Erythrocyte Membrane; D004912:Erythrocytes / Q000032:analysis*; D010710:Phosphates
chemical_list: D010710:Phosphates