Quantitative determination of oestradiol-17 beta hydroxysteroid dehydrogenase: increased sensitivity by HPLC separation of the hormones permits the measurement of enzyme activity in cryostat sections.
The activity of the oestradiol-17 beta hydroxysteroid dehydrogenase in human endometrial and breast cancer specimens was determined by the NAD-dependent conversion of oestradiol-17 beta to oestrone. The sensitivity of the determination was improved by the separation of the hormones by HPLC. We are now able to determine oestradiol-17 beta hydroxysteroid dehydrogenase quantitatively in cryostat sections. A clear correlation of serum progesterone levels and oestradiol-17 beta hydroxysteroid dehydrogenase activity in the endometrium was demonstrated, and we found a more than 30-fold increase in enzyme activity after the progesterone surge. In contrast, in breast cancer samples, we found no correlation between oestradiol-17 beta hydroxysteroid dehydrogenase and the measured serum parameters.
Vollmer
G
G
Institut für Biochemische Endokrinologie, Medizinische Universität zu Lübeck.
Wünsche
W O
WO
Knuppen
R
R
D015067:17-Hydroxysteroid Dehydrogenases / Q000032:analysis*; D014408:Biomarkers, Tumor / Q000032:analysis*; D001943:Breast Neoplasms / Q000175:diagnosis* / Q000201:enzymology / Q000473:pathology; D002851:Chromatography, High Pressure Liquid; D004796:Clinical Enzyme Tests; D004717:Endometrium / Q000201:enzymology / Q000473:pathology; D004958:Estradiol / Q000302:isolation & purification / Q000378:metabolism; D004960:Estradiol Dehydrogenases / Q000032:analysis* / Q000378:metabolism; D004970:Estrone / Q000302:isolation & purification; D005260:Female; D006801:Humans; D008598:Menstruation; D011374:Progesterone / Q000097:blood
D014408:Biomarkers, Tumor; D004970:Estrone; D011374:Progesterone; D004958:Estradiol; D015067:17-Hydroxysteroid Dehydrogenases; D004960:Estradiol Dehydrogenases