title: The acidification response of normal subjects to ammonium chloride using a 3-day loading test.
abstract: The acidification response to NH4 Cl loading (0.1 g/kg bw/day) was tested in 16 normal healthy subjects in the basal fasting state on Day 4, the subjects having taken the salt daily for the 3 previous days. The response was measured in terms of blood pH and in urine, creatinine, phosphate, pH, titratable acidity, ammonium, net acidity and creatinine clearance. To minimise inter-subject variation the urine values were adjusted to a standard body surface area of 1.73 m2. A normal range for the blood pH of the mean value +/- 2 SD, encompassed the observed range of values. However, to fit the observed range of acid-base values in urine into the 2 SD range required a logarithmic transformation of the data. Statistical analysis confirmed a significant correlation between blood [H+] net acid secretion, urine titratable acidity and ammonium. Urine net acid secretion was positively correlated with urinary phosphate, titratable acidity and ammonium.
authors.affiliation: Department of Chemical Pathology, Frimley Park Hospital, Camberley, Surrey, UK.
authors.forename: I D
authors.initials: ID
authors.lastname: Fry
authors.forename: P E
authors.initials: PE
authors.lastname: Cusick
authors.forename: W C
authors.initials: WC
authors.lastname: Alston
authors.forename: B
authors.initials: B
authors.lastname: Montgomery
authors.forename: J E
authors.initials: JE
authors.lastname: Tovey
mesh_terms: D000136:Acid-Base Equilibrium; D000328:Adult; D000367:Age Factors; D000643:Ammonium Chloride / Q000008:administration & dosage / Q000494:pharmacology*; D001769:Blood; D001830:Body Surface Area; D004305:Dose-Response Relationship, Drug; D004334:Drug Administration Schedule; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008297:Male; D008875:Middle Aged; D012737:Sex Factors; D014556:Urine / Q000032:analysis
chemical_list: D000643:Ammonium Chloride