Experimental study of invasion and metastasis of cancer cells and establishment of the morphological pattern in the process of invasion and metastatic models of cancer cells.
For the study of invasion of tumor cells in vitro and in vivo methods were used to observe the invasion process and morphological characteristics of tumor cells. The different animal tumor cells and cell lines from human tumors were used. The process of cancer cells in vitro invasion can be divided into 5 stages. We suggest that filopodia protruding from the tumor cell margin seem to play an important role in the initiation of cell invasion. For experimental study of cancer metastasis we established three spontaneous metastatic models, including spontaneous lymphatic and hematogenous metastasis of mouse uterine cervix carcinoma No 14(U14) and No 27(U27), and third spontaneous lung metastasis in mouse forestomach carcinoma (FC). Two tumor cell lines are established: One is MFC cell line which is derived from FC tumor; second cell line is MUCC which is derived from the U27 tumor. These metastasis models and their cell lines are very useful for studying the mechanism of metastasis, tumor heterogenicity and antimetastatic agents of tumor. The results are discussed.
Gao
J
J
Department of Pathology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Beijing.
D000818:Animals; D002460:Cell Line; D008207:Lymphatic Metastasis / Q000473:pathology; D051379:Mice; D008819:Mice, Nude; D009361:Neoplasm Invasiveness / Q000473:pathology*; D009362:Neoplasm Metastasis / Q000473:pathology*; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000473:pathology*; D051381:Rats; D014407:Tumor Cells, Cultured