The effect of diethylcarbamazine on the in vitro serum-mediated adherence of feline granulocytes to microfilariae of Brugia pahangi.
The effect of the antifilarial drug diethylcarbamazine (DEC) on immunologically mediated destruction of the microfilariae (mf) of Brugia pahangi in vitro is addressed by studying the drug's influence on the granulocyte adherence to the surface of mf and the subsequent death of these parasites. DEC enhanced the adherence of cat blood-derived granulocytes to the surface of mf through the meditation of cuticle direct antibodies but not that mediated by complement alone; the parasites became immobilized and degenerated subsequent to cell adherence. The rate of cell adherence in the antibody-containing cultures was enhanced by DEC. As previously reported, this drug had no direct effect on the viability of the parasites. Preincubation of the cells in DEC had a greater effect on cell adherence than did preincubating the parasites in the drug, thus supporting the contention that this antifilarial compound may act on host components. Further investigation of this possibility may contribute significantly to a more complete understanding of why DEC can cause severe side reactions in filariae infected patients.
Johnson
P
P
London School of Hygiene and Tropical Medicine.
Mackenzie
C D
CD
Denham
D A
DA
Suswillo
R R
RR
D000818:Animals; D002009:Brugia / Q000187:drug effects* / Q000276:immunology; D002415:Cats; D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D003165:Complement System Proteins / Q000276:immunology; D004049:Diethylcarbamazine / Q000494:pharmacology*; D006098:Granulocytes / Q000187:drug effects* / Q000276:immunology; D007106:Immune Sera / Q000276:immunology; D008842:Microfilariae / Q000187:drug effects / Q000276:immunology
D007106:Immune Sera; D003165:Complement System Proteins; D004049:Diethylcarbamazine