Analysis of water-soluble vitamins by micellar electrokinetic capillary chromatography.
Seven water-soluble vitamins were determined simultaneously by micellar electrokinetic capillary chromatography with UV detection. All these compounds were separated from each other within ca. 22 min by using a carrier containing sodium dodecyl sulphate as the surfactant. On-column detection at 254 nm with ethyl p-aminobenzoate as the internal standard allowed sensitive, accurate and reproducible determination of these compounds. Five principal constituents of a vitamin injection were determined with relative standard deviations of less than 2.1%.
Fujiwara
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S
Pharmaceuticals Research Center, Kanebo, Ltd., Osaka, Japan.
Iwase
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Honda
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S
D002845:Chromatography; D002851:Chromatography, High Pressure Liquid; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D008823:Micelles; D012967:Sodium Dodecyl Sulfate; D012995:Solubility; D013056:Spectrophotometry, Ultraviolet; D014815:Vitamins / Q000032:analysis*
D007202:Indicators and Reagents; D008823:Micelles; D014815:Vitamins; D012967:Sodium Dodecyl Sulfate