title: The inhibition by chlorate of the sulphation of polyethyleneglycol in the isolated perfused guinea pig liver.
abstract: 1. The sulphation of polyethyleneglycol 200 by the isolated perfused guinea pig liver is inhibited to about 60% by 10 mM ClO3- in the plasma of the perfusate when the concentration of SO4(2-) therein is 1.18 mM. 2. The inhibition is almost complete when the concentration of SO4(2-) is about 0.1 mM, a level which can be achieved by using a modified Ringer-bicarbonate solution, devoid of sulphate, to prepare the perfusate. 3. Chlorate, presumably through its action on ATP-sulphurylase, may therefore be a useful inhibitor of sulphation in the isolated perfused liver when the activity of the sulphurylase is rate-limiting. 4. The rate of bile production in the presence of chlorate is no different from that in its absence showing that, in the time scale of the perfusion, chlorate is not a general liver poison. 5. When the synthesis of PAPS is not rate-limiting, as in the sulphation of oestrone metabolites by rat liver, chlorate has no effect on the rate of sulphation.
authors.affiliation: Department of Biochemistry, University College, Cardiff, UK.
authors.forename: A B
authors.initials: AB
authors.lastname: Roy
authors.forename: C G
authors.initials: CG
authors.lastname: Curtis
authors.forename: G M
authors.initials: GM
authors.lastname: Powell
mesh_terms: D000818:Animals; D001646:Bile / Q000378:metabolism; D002704:Chlorates / Q000494:pharmacology*; D004970:Estrone / Q000378:metabolism; D006168:Guinea Pigs; D066298:In Vitro Techniques; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D010477:Perfusion; D011092:Polyethylene Glycols / Q000378:metabolism*; D012016:Reference Values; D013431:Sulfates / Q000378:metabolism*
chemical_list: D002704:Chlorates; D013431:Sulfates; D004970:Estrone; D011092:Polyethylene Glycols