title: Cholinergic muscarinic receptors in rat cochlea.
abstract: Specific 3H-1-quinuclidinylbenzilate (3H-1-QNB) binding to rat cochlea homogenates occurs to a homogeneous class of binding sites with Kd = 0.13 +/- 0.01 nM and Bmax = 0.57 +/- 0.07 fmol per cochlea. Binding is stereoselectively inhibited by benzetimide enantiomers. Dexetimide was more effective than levetimide in displacing 3H-1-QNB from its binding sites (Ki = 4 x 10(-10) M and 6.5 x 10(-6) M, respectively). Pirenzepine inhibits 3H-1-QNB binding with low affinity (Ki = 2 x 10(-6) M), classifying the binding sites as muscarinic M2 receptors.
authors.affiliation: Department of Otorhinolaryngology, University of Nijmegen, The Netherlands.
authors.forename: Y J
authors.initials: YJ
authors.lastname: van Megen
authors.forename: A B
authors.initials: AB
authors.lastname: Klaassen
authors.forename: J F
authors.initials: JF
authors.lastname: Rodrigues de Miranda
authors.forename: W
authors.initials: W
authors.lastname: Kuijpers
mesh_terms: D000818:Animals; D003051:Cochlea / Q000378:metabolism*; D011813:Quinuclidinyl Benzilate / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D011976:Receptors, Muscarinic / Q000378:metabolism*
chemical_list: D011976:Receptors, Muscarinic; D011813:Quinuclidinyl Benzilate