Purification of human 6-phosphogluconate dehydrogenase from human haemolysate with chromatography on an immobilized dye as the essential step and use of automation. Simultaneous purification of lactate dehydrogenase.
The screening procedure described in the preceding paper allowed a practical purification procedure to be devised that was automated for human 6-phosphogluconate dehydrogenase. The purification needed only two chromatographic steps, first on immobilized Procion Blue HE-GN and then on Phenyl-Sepharose. This technique also gave purified lactate dehydrogenase. Both enzymes showed single bands in SDS polyacrylamide gel electrophoresis.
Kroviarski
Y
Y
INSERM U160, Hôpital Beaujon, Clichy, France.
Cochet
S
S
Vadon
C
C
Truskolaski
A
A
Boivin
P
P
Bertrand
O
O
D002021:Buffers; D002852:Chromatography, Ion Exchange; D004396:Coloring Agents; D004591:Electrophoresis, Polyacrylamide Gel; D006461:Hemolysis; D006801:Humans; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000097:blood* / Q000302:isolation & purification; D009711:Nucleotides / Q000302:isolation & purification; D010734:Phosphogluconate Dehydrogenase / Q000097:blood* / Q000302:isolation & purification
D002021:Buffers; D004396:Coloring Agents; D007527:Isoenzymes; D009711:Nucleotides; D007770:L-Lactate Dehydrogenase; D010734:Phosphogluconate Dehydrogenase