title: A new application of in situ hybridization: detection of numerical and structural chromosome aberrations with a combination centromeric-telomeric DNA probe.
abstract: Fluorescent in situ hybridization provides a fast method for detection of specific nucleic acid sequences. We have used high-resolution, single-color fluorescent in situ hybridization with a combination centromeric-telomeric DNA probe, specific for chromosome 1, to investigate the feasibility of simultaneous assessment of numerical and structural chromosome aberrations. The K562 leukemia cell line served as a model.
authors.affiliation: Radiobiological Institute TNO, Rijswik, The Netherlands.
authors.forename: H
authors.initials: H
authors.lastname: van Dekken
authors.forename: J G
authors.initials: JG
authors.lastname: Bauman
mesh_terms: D002503:Centromere / Q000648:ultrastructure*; D002869:Chromosome Aberrations; D002875:Chromosomes / Q000648:ultrastructure*; D002878:Chromosomes, Human, Pair 1; D015342:DNA Probes; D006801:Humans; D008856:Microscopy, Fluorescence; D009693:Nucleic Acid Hybridization; D014407:Tumor Cells, Cultured
chemical_list: D015342:DNA Probes