title: Hemoglobinometry: evaluation of a new method with a stable primary standard.
abstract: Using purified chlorohemin as a stable standard, a newly described hemoglobinometry method was evaluated. All blood samples as well as chlorohemin gave identical absorption spectra in an alkaline-detergent solution with a broad peak at 575 nm. Both the reagent mixture and the chlorohemin standard were stable for at least 250 days when stored between 8 degrees C and 32 degrees C in brown glass bottles. The new method showed close correlation when compared to the cyanmethemoglobin technique. Compared to the cyanmethemoglobin method the advantages included a stable standard, shorter conversion time, reduced plasma background and improved hemoglobin ligands conversion. We conclude that the new method confers significant advantages over the cyanmethemoglobin method and in particular provides a stable primary standard with a long shelf-life.
authors.affiliation: Red Cross Blood Transfusion Service, Sydney.
authors.forename: B R
authors.initials: BR
authors.lastname: Wylie
authors.forename: V A
authors.initials: VA
authors.lastname: Lovric
mesh_terms: D006427:Hemin / Q000378:metabolism; D006452:Hemoglobinometry / Q000379:methods*; D006454:Hemoglobins / Q000032:analysis / Q000378:metabolism; D006801:Humans
chemical_list: D006454:Hemoglobins; D006427:Hemin