Effects of trembler mouse serum and laminin on oligodendrocyte proliferation and differentiation in culture.
Oligodendrocytes in primary cultures derived from rat embryo spinal cord were examined in control medium and in Trembler mouse serum (TMS)-supplemented medium. The oligodendrocytes were identified on the basis of the synthesis and surface expression of galactocerebrosides revealed by a monoclonal antibody directed against this component. We noticed two effects of TMS compared to control mouse serum. First, our results revealed that in TMS medium there is a mitogenic response of galactocerebroside (GalC)-positive cells. Second, in the presence of TMS, oligodendrocytes do not develop processes as they do in the presence of normal mouse serum. When laminin, a basal lamina component was added to TMS medium, GalC+ oligodendrocytes decreased in number and differentiation was normal. Possible explanations of the effects of TMS and laminin on oligodendrocyte proliferation and differentiation are discussed.
Hantaz-Ambroise
D
D
INSERM U.153, Paris, France.
Vigny
M
M
Alliot
F
F
D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D007797:Laminin / Q000494:pharmacology*; D051379:Mice; D008818:Mice, Neurologic Mutants / Q000097:blood*; D009457:Neuroglia / Q000502:physiology*; D009836:Oligodendroglia / Q000187:drug effects / Q000502:physiology*; D013116:Spinal Cord / Q000166:cytology / Q000196:embryology*
D007797:Laminin