N-succinyl-alanyl-methionyl-S-benzylcysteine p-nitroanilide as a sensitive substrate for assaying activity of cysteine proteinases.
N-Succinyl-alanyl-methionyl-S-benzylcysteine p-nitroanilide has been found to be a very sensitive chromogenic substrate for the assay of cysteine proteinase papain, ficin and bromelain. N-Succinyl-alanyl-S-benzylcysteine p-nitroanilide and N-succinyl-alanyl-alanyl-S-benzylcysteine p-nitroanilide are also suitable for this purpose. These substrates were hydrolyzed only very slightly or not hydrolyzed at all by trypsin.
Fukal
L
L
Department of Physiology, University of Agriculture, Prague, Czechoslovakia.
Kasafírek
E
E
D002863:Chromogenic Compounds; D003546:Cysteine Endopeptidases / Q000378:metabolism*; D007700:Kinetics; D009842:Oligopeptides; D013053:Spectrophotometry / Q000379:methods; D013379:Substrate Specificity
D002863:Chromogenic Compounds; D009842:Oligopeptides; C058609:N-succinyl-alanyl-methionyl-S-benzylcysteine-4-nitroanilide; D003546:Cysteine Endopeptidases