title: DNA-fluorescence of mammalian intra-nucleolar chromatin detected by confocal laser scanning microscopy (CLSM).
abstract: The complex spatial DNA distribution in the mammalian interphase nucleus was investigated in Feulgen stained thick sections through mouse trophoblast giant nuclei after Lowicryl embedding. DNA-fluorescence was visualized using confocal laser scanning microscopy. Our results show that the spatial arrangement of major interphase chromatin areas can be precisely documented, including the distribution of small intra-nucleolar chromatin zones.
authors.affiliation: Institute of Experimental Pathology, German Cancer Research Center, Heidelberg, Federal Republic of Germany.
authors.forename: H
authors.initials: H
authors.lastname: Spring
authors.forename: M F
authors.initials: MF
authors.lastname: Trendelenburg
authors.forename: M
authors.initials: M
authors.lastname: Montag
mesh_terms: D000818:Animals; D002466:Cell Nucleolus / Q000648:ultrastructure*; D002843:Chromatin / Q000032:analysis*; D004247:DNA; D007834:Lasers; D051379:Mice; D008855:Microscopy, Electron, Scanning / Q000379:methods*; D008856:Microscopy, Fluorescence; D014327:Trophoblasts / Q000648:ultrastructure
chemical_list: D002843:Chromatin; D004247:DNA