Modulation of vascular tone by 12(R)-, but not 12(S)-, hydroxyeicosatetraenoic acid.
The vascular activity of the two stereoisomers of 12-hydroxyeicosatetraenoic acid (12-HETE) was assessed on rabbit thoracic aortic rings. In vessels contracted in K+-free media, 12(R)-HETE inhibited the relaxations produced by the addition of KCl, in a concentration-dependent manner, while 12(S)-HETE had little effect. 12(R)-HETE, but not the 12(S) isomer, potentiated phenylephrine-induced contractions of aortic rings in normal buffer. Thus 12(R)-HETE can modulate vascular tone, perhaps by inhibition of Na+,K+-ATPase activity.
Masferrer
J
J
Department of Pharmacology, New York Medical College, Valhalla 10595.
Mullane
K M
KM
D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; D000818:Animals; D006893:Hydroxyeicosatetraenoic Acids / Q000494:pharmacology*; D008297:Male; D009126:Muscle Relaxation / Q000187:drug effects; D009129:Muscle Tonus / Q000187:drug effects; D009131:Muscle, Smooth, Vascular / Q000187:drug effects*; D010656:Phenylephrine / Q000494:pharmacology; D011188:Potassium / Q000494:pharmacology; D011817:Rabbits; D013237:Stereoisomerism
D006893:Hydroxyeicosatetraenoic Acids; D010656:Phenylephrine; D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; D011188:Potassium