Transforming growth factor beta stimulates phosphoinositol metabolism and translocation of protein kinase C in cultured astrocytes.
Transforming growth factor beta (TGF-beta) is a regulatory peptide found in many normal and neoplastic tissues, including brain, with a diverse range of cellular effects. The transmembrane biochemical signals by which TGF-beta exerts these effects and the second messenger systems that may amplify them are unknown. We investigated the effects of TGF-beta upon membrane phosphoinositol metabolism and protein kinase C activity in cultured astrocytes. We found that exposure of astrocyte enriched cultures to TGF-beta resulted in the stimulation of phosphoinositol lipid turnover to inositol phosphates and in the apparent redistribution of protein kinase C from cytosol to membrane.
Robertson
P L
PL
Department of Pediatrics, University of Michigan, Ann Arbor 48109.
Markovac
J
J
Datta
S C
SC
Goldstein
G W
GW
D000818:Animals; D001253:Astrocytes / Q000378:metabolism*; D002478:Cells, Cultured; D007295:Inositol Phosphates / Q000378:metabolism*; D011493:Protein Kinase C / Q000378:metabolism*; D051381:Rats; D013403:Sugar Phosphates / Q000378:metabolism*; D013997:Time Factors; D015291:Transforming Growth Factors / Q000494:pharmacology*
D007295:Inositol Phosphates; D013403:Sugar Phosphates; D015291:Transforming Growth Factors; D011493:Protein Kinase C