title: Circular-dichroism studies on two murine serum amyloid A proteins.
abstract: C.d. studies have shown that mouse SAA2 (serum amyloid A2) protein has about one-half of the alpha-helix content of the SAA1 (serum amyloid A1) analogue (15 as against 32%), although secondary-structure prediction analyses based on sequence data do not suggest such a large difference between the forms. The decreased helical content may be a reflection or indication of a stronger propensity to aggregation of the SAA2 form compared with SAA1. The main elements of secondary structure in both proteins are beta-sheets/turns. Interactions with Ca2+ are accompanied by small losses in alpha-helix content, whereas binding to chondroitin-6-sulphate in the presence of millimolar Ca2+ also decreases the amount of secondary structure. However, SAA2 binding to heparan sulphate increases its beta-sheet structure, whereas with SAA1 secondary structure is not apparently altered by its interaction with heparan sulphate. Computer-generated surface profiles show slight differences in accessibility, hydrophilicity and flexibility between the proteins. Understanding these differences may help to explain why SAA2 is found in amyloid fibrils whereas SAA1 is not. In particular, a stronger tendency to aggregation might be the reason why SAA2 is deposited exclusively in these fibrils.
authors.affiliation: Department of Biochemistry, University of Alberta, Edmonton, Canada.
authors.forename: W D
authors.initials: WD
authors.lastname: McCubbin
authors.forename: C M
authors.initials: CM
authors.lastname: Kay
authors.forename: S
authors.initials: S
authors.lastname: Narindrasorasak
authors.forename: R
authors.initials: R
authors.lastname: Kisilevsky
mesh_terms: D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002942:Circular Dichroism; D006025:Glycosaminoglycans / Q000494:pharmacology; D051379:Mice; D011487:Protein Conformation / Q000187:drug effects; D000685:Serum Amyloid A Protein
chemical_list: D006025:Glycosaminoglycans; D000685:Serum Amyloid A Protein; D002118:Calcium