title: Serodiagnosis of Streptococcus pneumoniae infection in guinea pigs by an enzyme-linked immunosorbent assay.
abstract: Guineapig antibodies to Streptococcus pneumoniae (SPN) serotype 19F were detected by an enzyme-linked immunosorbent assay using a simple procedure. In experimentally infected hosts, antibody was detectable as early as 2 to 3 weeks after infection, and high titres were maintained for a long period. Antibodies higher than 1:64 were regarded as specific. In a field study, high antibody titres were shown in SPN enzootic colonies in contrast to negative or low antibody titres in a majority of the animals from non-enzootic and SPF colonies.
authors.affiliation: Department of Veterinary Science, National Institute of Health, Tokyo, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Matsubara
authors.forename: T
authors.initials: T
authors.lastname: Kamiyama
authors.forename: T
authors.initials: T
authors.lastname: Miyoshi
authors.forename: H
authors.initials: H
authors.lastname: Ueda
authors.forename: M
authors.initials: M
authors.lastname: Saito
authors.forename: M
authors.initials: M
authors.lastname: Nakagawa
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary*; D006168:Guinea Pigs / Q000276:immunology*; D011008:Pneumococcal Infections / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D012376:Rodent Diseases / Q000175:diagnosis*; D012698:Serologic Tests / Q000662:veterinary*
chemical_list: D000907:Antibodies, Bacterial