Dose-dependence of endoneurial fluid sodium and chloride accumulation in galactose intoxication.
Endoneurial edema in galactose neuropathy was studied in a colony of Sprague-Dawley rats fed diets containing 0%, 10%, 20% or 40% D-galactose for approx. 200 days. Endoneurial fluid was analyzed by X-ray microanalysis for electrolyte concentration, by microgravimetry of whole nerve segments for water content, by measurement of endoneurial fluid pressure and by morphometry in transverse sections of nerve. Galactose intoxication resulted in dose-dependent increases in endoneurial fluid sodium and chloride that were directly associated with increases in nerve water content and endoneurial fluid pressure. The presence of edema and its dose-dependence was also confirmed by morphometric analysis of sciatic nerves at the light microscopic level. The data demonstrate that electrolyte-induced osmotic imbalances in endoneurial fluid are dependent on the amount of galactose ingested and suggest that the dose-related accumulation of sodium and chloride in endoneurial fluid contributes substantially to the pathogenesis of galactose neuropathy.
Mizisin
A P
AP
Department of Pathology (Neuropathology), University of California, School of Medicine, San Diego 92093.
Myers
R R
RR
Heckman
H M
HM
Powell
H C
HC
D000818:Animals; D002712:Chlorides / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D005110:Extracellular Space / Q000187:drug effects / Q000502:physiology*; D005690:Galactose / Q000633:toxicity*; D009498:Neurotoxins; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values; D012584:Sciatic Nerve / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D012964:Sodium / Q000378:metabolism*; D014882:Water-Electrolyte Balance / Q000187:drug effects*
D002712:Chlorides; D009498:Neurotoxins; D012964:Sodium; D005690:Galactose