title: Accurate and specific HPLC assay of coproporphyrinogen III oxidase activity in human peripheral leucocytes.
abstract: An HPLC method is described for the assay of coproporphyrinogen III oxidase in human leucocytes. The optimal pH for the assay was 6.5-7.0 and the Km for coproporphyrinogen III was 0.12 +/- 0.021 mumol/l. The mean activity in 28 apparently health subjects was 0.249 (2 SD range 0.130-0.368) nmol/h per mg protein. In two patients with hereditary coproporphyria, the activities were 0.029 and 0.078 nmol/h per mg protein.
authors.affiliation: Division of Clinical Cell Biology, MRC Clinical Research Centre, Harrow, Middlesex, UK.
authors.forename: R
authors.initials: R
authors.lastname: Guo
authors.forename: C K
authors.initials: CK
authors.lastname: Lim
authors.forename: T J
authors.initials: TJ
authors.lastname: Peters
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002851:Chromatography, High Pressure Liquid; D003304:Coproporphyrinogen Oxidase / Q000097:blood*; D006801:Humans; D007962:Leukocytes / Q000201:enzymology*; D008875:Middle Aged; D010088:Oxidoreductases / Q000097:blood*; D012016:Reference Values
chemical_list: D010088:Oxidoreductases; D003304:Coproporphyrinogen Oxidase