title: [Ion pair-HPLC of some aromatic amino- and hydroxycarboxylic acids].
abstract: Various factors influencing the ion-pair-HPLC separation of some aromatic amino- and hydroxycarbon acids were described and discussed. Distinct effects of the ion pair formation of organic acids with quarternary alkylammonium salts (CTAB) are recognized on condition that the carboxylic group is not blocked by intramolecular H-bonding or partial betain structure. If the carboxylic group is unconnected the retention time increases depending on the pka of the acid with increasing pH if no complete ionization exists. In order to separate similar compounds at low retention time with distinct resolution an organic modifier of suitable strength and high selectivity have to be added to the mobile phase.
authors.affiliation: Sektion Pharmazie der Ernst-Moritz-Arndt-Universität Greifswald, Wissenschaftsbereich Pharmazeutische Chemie.
authors.forename: T
authors.initials: T
authors.lastname: Jira
authors.forename: T
authors.initials: T
authors.lastname: Beyrich
authors.forename: K
authors.initials: K
authors.lastname: Reinhardt
mesh_terms: D002021:Buffers; D002264:Carboxylic Acids / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D006863:Hydrogen-Ion Concentration
chemical_list: D002021:Buffers; D002264:Carboxylic Acids
vernacular_title: Untersuchungen zur Ionenpaar-HPLC einiger aromatischer Amino- und Hydroxycarbonsäuren.