[Cytochrome P-450-dependent mechanisms of the biosynthesis of protein-conjugated benzo(a)pyrene antigens and their role in the development of a specific immune response to this carcinogen].
There was studied the possibility of covalent binding to proteins of a carcinogenic compound benzo(a)pyrene in the system of cytochrome P-450 of the liver and the possibility of the development of the immune reaction to administration of the conjugated antigens obtained in such a way to animals. It was shown that under experimental conditions modelling the processes of microsomal oxidation of benzo(a)pyrene in the organism there occurs irreversible binding of 14C-benzo(a)pyrene both to microsomes and the added albumin. Immunization of rabbits by conjugates benzo(a)pyrene-albumin and benzo(a)pyrene-microsomes leads to the development of the immune response to the original carcinogen. The antibodies and lymphocytes specifically binding 14C-benzo(a)pyrene appear in the blood.
Tomilina
N Iu
NIu
Shipulina
N V
NV
Kovalev
I E
IE
D000818:Animals; D000917:Antibody Formation; D000941:Antigens / Q000276:immunology*; D001564:Benzo(a)pyrene / Q000276:immunology* / Q000493:pharmacokinetics; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis*; D004790:Enzyme Induction; D006241:Haptens / Q000276:immunology*; D007114:Immunization; D008297:Male; D008748:Methylcholanthrene; D008862:Microsomes, Liver / Q000201:enzymology; D010084:Oxidation-Reduction; D011485:Protein Binding; D011817:Rabbits
D000941:Antigens; D006241:Haptens; D001564:Benzo(a)pyrene; D008748:Methylcholanthrene; D003577:Cytochrome P-450 Enzyme System
Izuchenie tsitokhrom-P-450-zavisimykh mekhanizmakh biosinteza kon''iugirovannykh antigenov benzo(a)pirena s belkami i ikh rol' v razvitii spetsificheskogo immunnogo otveta na étot kantserogen.