Speculative cell cycle in yeast-phase growth of Sporothrix schenckii: plasma membrane ultrastructure as revealed by freeze-fracture electron microscopy.
The cell cycle in yeast-phase growth of Sporothrix schenckii was investigated by light microscopy and freeze-fracture electron microscopy after a 3- to 7-day cultivation on brain heart infusion agar medium at 37 degrees C. Mother yeastlike cells were able to bear daughter yeastlike cells. They were also able to produce germ tubes that had the potential to develop into pseudohyphae and hyphae. On the other hand, hyphae or pseudohyphae born from yeastlike cells were able to bear yeastlike cells directly. These results lead us to propose a hypothetical cell cycle for yeast-phase growth involving yeastlike vegetative cells, pseudohyphae, and hyphae.
Maeda
M
M
Department of Dermatology, Gifu University School of Medicine, Japan.
Kitajima
Y
Y
Mori
S
S
D002453:Cell Cycle; D002462:Cell Membrane / Q000648:ultrastructure; D003470:Culture Media; D005614:Freeze Fracturing; D008854:Microscopy, Electron; D013175:Sporothrix / Q000166:cytology* / Q000254:growth & development / Q000648:ultrastructure
D003470:Culture Media