A novel nuclear transcription system which responds correctly to cloned estrogen receptor.
We describe here a novel but simple nuclear transcription system in which nuclei make RNA in an isotonic buffer for a long time and respond to a cloned external factor by accurately initiating new transcription.
Thorburn
A
A
Department of Biochemistry, Oxford, UK.
Knowland
J
J
D000818:Animals; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism*; D003001:Cloning, Molecular; D066298:In Vitro Techniques; D007700:Kinetics; D008099:Liver / Q000378:metabolism; D008244:Lysophosphatidylcholines / Q000494:pharmacology; D008297:Male; D011092:Polyethylene Glycols / Q000494:pharmacology; D011960:Receptors, Estrogen / Q000235:genetics / Q000502:physiology*; D011994:Recombinant Proteins / Q000494:pharmacology*; D014158:Transcription, Genetic / Q000187:drug effects; D014819:Vitellogenins / Q000235:genetics; D014981:Xenopus
D008244:Lysophosphatidylcholines; D011960:Receptors, Estrogen; D011994:Recombinant Proteins; D014819:Vitellogenins; D011092:Polyethylene Glycols