Calcium-binding lysozymes.
It was found that pigeon lysozyme binds one calcium ion, as does equine lysozyme. The protein was eluted with equimolar calcium ions from a Bio-Gel P-60 column. The binding constants of equine and pigeon lysozymes were determined to be 2 x 10(6) and 1.6 x 10(7) M-1, respectively, in 0.1 M KCl at pH 7.1 and 20 degrees C. During evolution the gene of calcium-binding lysozyme is deduced to be separated from that of non-calcium-binding lysozyme by gene duplication before splitting of avian and mammalian lineages, from their amino-acid sequences. It is assumed that the alpha-lactalbumin might have evolved from calcium-binding lysozyme.
Nitta
K
K
Department of Polymer Science, Faculty of Science, Hokkaido University, Japan.
Tsuge
H
H
Shimazaki
K
K
Sugai
S
S
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002135:Calcium-Binding Proteins; D002850:Chromatography, Gel; D010856:Columbidae; D004529:Egg White; D005260:Female; D007700:Kinetics; D009113:Muramidase / Q000302:isolation & purification / Q000378:metabolism*; D013045:Species Specificity
D002135:Calcium-Binding Proteins; D009113:Muramidase; D002118:Calcium