Activation of the adenovirus-2 E2a late promoter during inhibition of protein synthesis by cycloheximide.
The 5' termini of human adenovirus 2 (Ad2) E2a mRNA, which starts at two separate (early and late) promoter regions, were mapped on Ad2 nucleotide sequences with the aid of primer extension analysis and quantitated. In the early stage (6 h) of Ad2 infection of KB cells and 293 cells, E2a mRNA started mostly at the early (75.4 mp) promoter region. When these infected cells were cultured in the presence of cycloheximide from 1 to 6 h postinfection, the transcription from the late (72.2 mp) promoter region increased. Especially in 293 cells, approx. 50% of E2a mRNA started at the late promoter region. We conclude from the results that the activation of the late promoter during infection does not depend on viral DNA replication. Our results suggest the involvement of cellular protein(s) in this activation.
Kitani-Yasuda
T
T
Meiji Institute of Health Science, Odawara, Japan.
Yasuda
N
N
Hashimoto
S
S
D000260:Adenoviruses, Human / Q000187:drug effects / Q000235:genetics*; D001483:Base Sequence; D002460:Cell Line; D003513:Cycloheximide / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects*; D005814:Genes, Viral / Q000187:drug effects*; D006801:Humans; D007624:KB Cells; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D011401:Promoter Regions, Genetic / Q000187:drug effects*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics; D014158:Transcription, Genetic
D012333:RNA, Messenger; D003513:Cycloheximide