In situ hybridisation and S1 mapping show that the presence of infiltrating plasma cells is associated with poor prognosis in breast cancer.
In order to identify potential markers of prognosis in breast cancer, representative cDNA libraries were constructed using RNA isolated from primary breast tumour tissue associated with good and poor prognosis. Cross-screening of these libraries repeatedly identified cloned mRNA species associated with the immune system, in particular B-cells, in libraries derived from tumours of poor prognosis. We have used one of these a kappa IV light chain cDNA probe, in two complementary studies to investigate the relationship between immunoglobin gene expression and prognosis. The results obtained using a combination of S1 mapping, RNA blotting and in situ hybridisation demonstrate that the presence of plasma cells, as defined by infiltrating cells which express high levels of immunoglobulin kappa-chain mRNA, is associated with a poor prognosis.
Parkes
H
H
Cancer Research Campaign Tumour Endocrinology Group, Medical Molecular Biology Unit, University College and Middlesex School of Medicine, London, UK.
Collis
P
P
Baildam
A
A
Ralphs
D
D
Lyons
B
B
Howell
A
A
Craig
R
R
D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor; D001943:Breast Neoplasms / Q000235:genetics* / Q000276:immunology; D015342:DNA Probes; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D005803:Genes, Immunoglobulin; D006801:Humans; D008875:Middle Aged; D009693:Nucleic Acid Hybridization; D010950:Plasma Cells / Q000473:pathology*; D011061:Poly A / Q000032:analysis; D011379:Prognosis; D012333:RNA, Messenger / Q000096:biosynthesis; D012334:RNA, Neoplasm / Q000032:analysis; D011948:Receptors, Antigen, T-Cell / Q000276:immunology; D015183:Restriction Mapping
D014408:Biomarkers, Tumor; D015342:DNA Probes; D004273:DNA, Neoplasm; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D011948:Receptors, Antigen, T-Cell; D011061:Poly A