Immunohistochemical investigation on the hog kidney di-isopropyl-fluorophosphate fluorohydrolase (E.C.3.8.2.1) in hog kidney and heart.
Using a polyclonal antibody raised in rabbits against the highly purified di-isopropyl-fluorophosphate fluorohydrolase (DFPase, E.C. 3.8.2.1) of hog kidney, DFPase-immunoreactivity could be demonstrated by the unlabelled antibody peroxidase-antiperoxidase immunohistochemical method in the hog kidney in the brush border of epithelial cells in the proximal segment of the nephron. In the heart DFPase-immunoreactivity was found at the plasma membranes of the cardiac muscle cells.
Schindelmeiser
J
J
Institute of Anatomy, University of Münster, Federal Republic of Germany.
Klumpe
M
M
Storkebaum
W
W
Witzel
H
H
D000818:Animals; D002462:Cell Membrane / Q000201:enzymology; D004950:Esterases; D006867:Hydrolases / Q000378:metabolism*; D007150:Immunohistochemistry; D007668:Kidney / Q000201:enzymology*; D007687:Kidney Tubules, Proximal / Q000201:enzymology; D008871:Microvilli / Q000201:enzymology; D009206:Myocardium / Q000201:enzymology; D044345:Phosphoric Triester Hydrolases; D013552:Swine
D006867:Hydrolases; D004950:Esterases; D044345:Phosphoric Triester Hydrolases; C020061:diisopropyl-fluorophosphatase