Fast isoelectric focusing of some polymorphic proteins and enzymes in miniaturized gels using an automated system.
Optimal programs for the separation of polymorphic proteins and enzymes in miniaturized polyacrylamide gels using an automated system (PhastSystem) are described. The potential advantages and disadvantages of the method and its application to forensic science laboratories are discussed.
Carracedo
A
A
Department of Legal Medicine, University of Santiago de Compostela, Galicia, Spain.
Andrade-Vide
C
C
Rodriguez-Calvo
M S
MS
Montiel
M D
MD
Lareu
M V
MV
D000180:Acrylic Resins; D001331:Automation; D004798:Enzymes / Q000032:analysis*; D005554:Forensic Medicine / Q000379:methods; D006801:Humans; D007525:Isoelectric Focusing / Q000379:methods*; D008904:Miniaturization; D010641:Phenotype; D011110:Polymorphism, Genetic; D011506:Proteins / Q000032:analysis*
D000180:Acrylic Resins; D004798:Enzymes; D011506:Proteins; C016680:polyacrylamide gels