The significance of HLA studies in human-human hybridomas.
The technique of human-human (H + H) hybridoma is employed to generate human monoclonal antibodies. However, in contrast to mouse hybridomas, the human counterpart is very difficult to establish. It is still unclear what the precise reasons are for the failure to establish the human-human hybridoma technique as a routine. Analyses of HLA antigens of seven lymphocyte donors and 22 human cell lines generated by the H + H hybridoma technique have demonstrated the importance of the compatibility between human lymphocytes and the lymphoblastoid cell lines. Furthermore, there is a preferential expression of several HLA on human hybridomas (e.g. B51; B15; B18; B35). Screening several unrelated human lymphoblastoid cell lines available demonstrated preferred HLA antigens (A2, A3; A30/31; B5; B18; B15; B35), despite the fact that these lines were derived from subjects of different ethnic origins. It seems that HLA typing of donor lymphocytes and lymphoblastoid cell lines may help to increase the yield of H + H hybridomas.
Shoenfeld
Y
Y
Department of Medicine D, Corob Research Center, Soroka Medical Center, Faculty of Health Sciences, Ben-Gurion University, Beer-Sheva, Israel.
Zamir
R
R
D002459:Cell Fusion; D006680:HLA Antigens / Q000276:immunology*; D006801:Humans; D006825:Hybridomas / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology
D006680:HLA Antigens