Thin filaments of rabbit skeletal muscle are in helical register.
Thin sections of rapidly frozen and freeze-substituted rabbit glycerinated muscle fibres loaded with myosin subfragment-1 were used to examine a three-dimensional arrangement of thin filaments in vertebrate skeletal muscle. Clearer images of the "arrowhead" structure were obtained when specimens were freeze-substituted first in a tannic acid solution and then in an OsO4 solution. The images obtained showed that the arrowheads were aligned laterally. This indicates that all the thin filaments have the same rotational orientation in a half sarcomere of rabbit skeletal muscle in the rigor state.
Hirose
K
K
Department of Physics, Faculty of Science, University of Tokyo, Japan.
Wakabayashi
T
T
D000199:Actins; D000818:Animals; D008954:Models, Biological; D009132:Muscles / Q000033:anatomy & histology*; D015879:Myosin Subfragments; D009218:Myosins; D010446:Peptide Fragments; D011817:Rabbits
D000199:Actins; D015879:Myosin Subfragments; D010446:Peptide Fragments; D009218:Myosins