title: Optical errors in a liquid chromatography absorbance cell.
abstract: The shadowgraph method is used to visualize mobile phase flow through a liquid chromatography absorbance cell with "Z" flow geometry. It is shown that mixing is incomplete in the cell. Composition gradients persist throughout the elution of any sample. The gradients systematically change as the sample flows through the cell. Additionally, window wedge angles are measured in several cells. The implications for low noise absorbance detectors are discussed.
authors.affiliation: Department of Chemistry, University of Michigan, Ann Arbor 48109.
authors.forename: K
authors.initials: K
authors.lastname: Peck
authors.forename: M D
authors.initials: MD
authors.lastname: Morris
mesh_terms: D001391:Azo Compounds / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation; D002853:Chromatography, Liquid / Q000295:instrumentation*; D007202:Indicators and Reagents; D012031:Refractometry
chemical_list: D001391:Azo Compounds; D007202:Indicators and Reagents; C100258:methyl orange