title: Determination of secondary structure in the initiation region of ovalbumin mRNA.
abstract: We have analyzed the secondary structure in the region surrounding the initiation codons of both cellular and synthetic versions of ovalbumin mRNA. RNase V1 cleavage sites and structure-dependent, chemically modified bases in cellular ovalbumin mRNA were determined by reverse transcription of hen poly A(+) RNA using ovalbumin-specific, synthetic DNA primers. These results indicate an extensive region of unpaired nucleotides preceding the initiation codon and a region of base-paired nucleotides including and following the initiation codon. A synthetic ovalbumin mRNA (SP65.OV) was prepared by run-off transcription of a cloned ovalbumin cDNA (pSP65.OV). Identical regions of hen ovalbumin and SP65.OV mRNAs gave identical patterns of structure-dependent base modifications. A computer program for determining RNA secondary structure was used to find a 5'-region structure for ovalbumin mRNA that is consistent with our data.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Arkansas for Medical Sciences, Little Rock 72205.
authors.forename: C D
authors.initials: CD
authors.lastname: Liarakos
authors.forename: R P
authors.initials: RP
authors.lastname: Maddox
authors.forename: K A
authors.initials: KA
authors.lastname: Hilscher
authors.forename: J R
authors.initials: JR
authors.lastname: Bishop
authors.forename: D K
authors.initials: DK
authors.lastname: McGuire
authors.forename: R A
authors.initials: RA
authors.lastname: Kopper
mesh_terms: D000818:Animals; D001482:Base Composition; D002645:Chickens; D003062:Codon; D004247:DNA / Q000302:isolation & purification; D004722:Endoribonucleases; D005260:Female; D008745:Methylation; D009690:Nucleic Acid Conformation; D010047:Ovalbumin / Q000235:genetics*; D010442:Peptide Chain Initiation, Translational; D012333:RNA, Messenger / Q000302:isolation & purification
chemical_list: D003062:Codon; D012333:RNA, Messenger; D010047:Ovalbumin; D004247:DNA; D004722:Endoribonucleases; C032825:ribonuclease V(1)