Particle migration from haemodialysis circuit: electron microscopy and microprobe analysis.
It has been recognized that storage inflammation in organs of uraemic patients is due to silicone particle migration from tubing segments of the haemodialysis circuit to blood. Nevertheless, iatrogenic storage of foreign material containing Si has been also observed in long-term dialysis patients which, in our Unit, used only PVC or PU-PVC tubings. The origin and the nature of the particulate has been investigated in vivo and in vitro on bioptical samples as well as on cuprophan dialyser and PVC tubing eluates. This study carried out by means of TEM, SEM and microprobe EDS revealed the presence of variously shaped material and particles containing Si in bioptical samples and in eluates. Si containing contaminants were not demonstrated in eluates filtered in absence of the dialyser. This result suggests that leachable products can result from the dialyser and that such release can be an additional risk for uraemic patients.
Caiazza
S
S
Department of Ultrastructures, Istituto Superiore di Sanità, Rome, Italy.
Giangrande
A
A
Cantù
P
P
Castiglioni
A
A
Paoletti
L
L
Donelli
G
G
D004577:Electron Probe Microanalysis; D006801:Humans; D007249:Inflammation / Q000209:etiology; D007688:Kidneys, Artificial / Q000009:adverse effects*; D008854:Microscopy, Electron; D012825:Silicon / Q000009:adverse effects / Q000302:isolation & purification*; D014511:Uremia / Q000150:complications / Q000628:therapy
D012825:Silicon