title: Decreased in vitro polymerase II activity of whole-cell extracts from late passage human diploid fibroblasts.
abstract: Age-associated decreases in gene expression have been observed in several different species, but the mechanisms of the decrease have not yet been adequately studied at molecular level. Using an in vitro transcription assay, we have obtained data suggesting that there is an age-associated decrease in polymerase II-specific transcriptional activity in fibroblasts aged in vitro.
authors.affiliation: Department of Microbiology and Immunology, UCLA School of Medicine 90024.
authors.forename: T C
authors.initials: TC
authors.lastname: Fong
authors.forename: T
authors.initials: T
authors.lastname: Makinodan
mesh_terms: D000375:Aging / Q000235:genetics*; D002460:Cell Line; D002478:Cells, Cultured; D004257:DNA Polymerase II / Q000235:genetics*; D005347:Fibroblasts / Q000201:enzymology; D014158:Transcription, Genetic
chemical_list: D004257:DNA Polymerase II