Microtiter method for MIC testing with spherule-endospore-phase Coccidioides immitis.
A method was developed for susceptibility testing with spherule-endospore-phase Coccidioides immitis by using a microtiter format. Isolated endospores were used to inoculate wells containing modified Converse medium with various concentrations of azole or nikkomycin antifungal substances which then were sealed with an acetate film. The plate was incubated at 37 degrees C with shaking for 96 h, after which the control wells had visible turbidity and endpoints were discernible. Microscopic examination revealed that both control and treatment wells maintained cells predominantly in the spherule-endospore phase of growth.
Hector
R F
RF
Cutter Biologicals, Berkeley, California 94701.
Zimmer
B L
BL
Pappagianis
D
D
D000935:Antifungal Agents / Q000494:pharmacology*; D003045:Coccidioides / Q000187:drug effects* / Q000502:physiology; D008826:Microbial Sensitivity Tests / Q000379:methods; D013171:Spores, Bacterial / Q000187:drug effects / Q000502:physiology
D000935:Antifungal Agents