Comparison of intraocular solutions on glycolysis and levels of ATP and glutathione in the retina.
The effects of several intraocular irrigating solutions on the rate of glycolysis and the levels of ATP and glutathione in the isolated rat retina were investigated. The media were HCO3-Ringer, balanced salt solution (BSS) (to which 5 mM glucose was added), and BSS PLUS. There were no significant differences in the metabolic activities and metabolite levels in retinas incubated with either HCO3-Ringer or BSS PLUS. Retinas incubated with BSS PLUS had higher rates of aerobic and anaerobic glycolysis and more ATP than did retinas incubated with BSS. There were no significant differences, however, in the glutathione content of retinas incubated with BSS PLUS and BSS. These results show that retinal energy metabolism is maintained better with BSS PLUS because this irrigating solution contains glucose and bicarbonate.
Winkler
B S
BS
Eye Research Institute, Oakland University, Rochester, Michigan 48309.
D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D005978:Glutathione / Q000378:metabolism*; D006019:Glycolysis / Q000187:drug effects; D066298:In Vitro Techniques; D009883:Ophthalmic Solutions / Q000494:pharmacology*; D051381:Rats; D012160:Retina / Q000378:metabolism*; D007507:Therapeutic Irrigation
D009883:Ophthalmic Solutions; D000255:Adenosine Triphosphate; D005978:Glutathione