Physicochemical characterization and tissue distribution of multiple molecular forms of fish (Trachurus trachurus) esterases.
1. Tissue-specific electrophoretic patterns of multiple molecular forms of esterases were observed in fish Trachurus trachurus. They were composed of three (in intestine) to nine (in liver) bands characterized mainly as carboxylesterases and acetylesterases. 2. Two major esterase activity bands of pI 4.60 and 4.77 were accompanied by minor ones of higher pH in tissue extracts. 3. Under optimum assay conditions, liver was the richest source of esterase activity followed by intestine, stomach, brain, red muscles, heart, white muscles and gills. 4. Esterases of 70,000 and 420,000 mol. wt were resolved by gel-filtration in liver, intestine and brain. Low size esterases prevailed in liver while the opposite was the case in brain.
Salamastrakis
S S
SS
Zoological Laboratory, Faculty of Science, University of Athens, Greece.
Haritos
A A
AA
D000818:Animals; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D004950:Esterases / Q000302:isolation & purification*; D005399:Fishes / Q000378:metabolism*; D007525:Isoelectric Focusing; D007526:Isoelectric Point; D008970:Molecular Weight; D014018:Tissue Distribution
D004950:Esterases