MFO activity changes in single living 3T3 fibroblasts treated with adriamycin.
The effects of Adriamycin on 3T3 fibroblasts has been followed by microspectrofluorimetry. MFO activity was monitored by BaP metabolism and three points were studied: long-term ADR treatment, reversibility of this treatment and cysteamine effect on ADR treatment. The appearance of a sub-population that does not accumulate BaP suggests either a greater efflux of BaP or no penetration of the compound after ADR treatment. Moreover drastic morphological changes were observed.
Lahmy
S
S
Laboratorie de Chimie Physique, Université de Perpignan, France.
Salmon
J M
JM
Viallet
P
P
D000818:Animals; D002453:Cell Cycle / Q000187:drug effects; D002478:Cells, Cultured; D003543:Cysteamine / Q000494:pharmacology; D004317:Doxorubicin / Q000494:pharmacology*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D007700:Kinetics; D051379:Mice; D006899:Mixed Function Oxygenases / Q000378:metabolism*
D003543:Cysteamine; D004317:Doxorubicin; D006899:Mixed Function Oxygenases