title: A polypeptide growth inhibitor isolated from lactating bovine mammary gland (MDGI) is a lipid-carrying protein.
abstract: Mammary-derived growth inhibitor (MDGI), a polypeptide growth inhibitor isolated from lactating bovine mammary tissue, previously shown to have extensive sequence homology with fatty acid-binding proteins, was demonstrated to meet the criteria of a fatty acid-binding protein. The protein was found to bind [3H]palmitic acid in a saturable manner and to be complexed with endogeneous free fatty acids. [3H]palmitic acid, when bound to the protein, was more rapidly taken up by the target cells (human mammary carcinoma cells [MaTu]) than was free [3H]palmitic acid, suggesting a lipid carrier function for the inhibitor. It is suggested that the fatty acid-binding properties of MDGI may relate to its ability to inhibit cell growth in vitro and to regulate other cellular functions.
authors.affiliation: Department of Cell Biology, Academy of Sciences of the G.D.R.
authors.forename: F D
authors.initials: FD
authors.lastname: Böhmer
authors.forename: M
authors.initials: M
authors.lastname: Mieth
authors.forename: G
authors.initials: G
authors.lastname: Reichmann
authors.forename: C
authors.initials: C
authors.lastname: Taube
authors.forename: R
authors.initials: R
authors.lastname: Grosse
authors.forename: M D
authors.initials: MD
authors.lastname: Hollenberg
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D002417:Cattle; D005227:Fatty Acids / Q000378:metabolism*; D005260:Female; D006131:Growth Inhibitors / Q000302:isolation & purification / Q000378:metabolism; D007774:Lactation; D008321:Mammary Glands, Animal / Q000032:analysis*; D011247:Pregnancy
chemical_list: D002352:Carrier Proteins; D005227:Fatty Acids; D006131:Growth Inhibitors