Study of doxorubicin photodegradation in plasma, urine and cell culture medium by HPLC.
An HPLC method was used to monitor the chemical stability of doxorubicin-HCl (DXR) to light in plasma, urine and cell culture medium at room temperature. The results indicated that DXR was very unstable in cell culture medium and urine when exposed to light. It was more stable in plasma under the same conditions. In all the cases, the decrease in the amount of DXR is greatly dependent on light intensity (no noticeable degradation was observed after 8 h in the dark). These observations may be important for the correct interpretation of the effects and the toxicity of doxorubicin on cells incubated in cell medium, and for determination of urinary or plasma pharmacokinetic parameters.
Le Bot
M A
MA
Laboratoire de Pharmacologie, Centre Regional de Pharmacovigilance C.H.U. Morvan, Brest, France.
Riche
C
C
Guedes
Y
Y
Kernaleguen
D
D
Simon
S
S
Begue
J M
JM
Berthou
F
F
D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D003630:Daunorubicin / Q000032:analysis; D004317:Doxorubicin / Q000097:blood / Q000378:metabolism* / Q000528:radiation effects / Q000652:urine; D007700:Kinetics; D010777:Photochemistry; D012015:Reference Standards; D013050:Spectrometry, Fluorescence
D004317:Doxorubicin; D003630:Daunorubicin