Casein kinases I and II bound to pig brain microtubules.
1. Microtubules prepared from pig brain by two cycles of assembly-disassembly comprise cyclic nucleotide-independent protein kinase activity with phosvitin and troponin T as substrates. 2. Phosphocellulose chromatography resolved two phosvitin kinase activity peaks, one of which coincided with the troponin T kinase peak. 3. The activity peak corresponding to troponin T kinase was inhibited by heparin (I50 = 0.06 micrograms/ml), whereas the other phosvitin kinase peak was unaffected. 4. Both kinase fractions phosphorylated tubulin and microtubule-associated protein (MAP-2). 5. It is concluded that pig brain microtubules contain bound casein kinases I and II. The association may target the action of these kinases toward microtubular proteins in vivo.
Risnik
V V
VV
Department of Biochemistry, School of Biology, Moscow State University, U.S.S.R.
Adám
G
G
Gusev
N B
NB
Friedrich
P
P
D000818:Animals; D001921:Brain / Q000201:enzymology*; D047388:Casein Kinases; D008868:Microtubule Proteins / Q000378:metabolism; D008870:Microtubules / Q000201:enzymology*; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism*; D013347:Subcellular Fractions; D013552:Swine / Q000378:metabolism*
D008868:Microtubule Proteins; D011494:Protein Kinases; D047388:Casein Kinases