Determination of 17-hydroxyprogesterone in plasma by gas chromatography-mass spectrometry with high-resolution selected-ion monitoring.
A method for determining 17-hydroxyprogesterone in plasma by isotope dilution-mass spectrometry is described. For the internal standard 17-hydroxy [2H4]progesterone is used. Extraction of plasma is followed by conversion into the 3,20-dienol,17-tristrimethylsilyl ether derivative and analysis by capillary gas chromatography-mass spectrometry with selected-ion monitoring, at a resolution of 6000. The lower limit of quantitation was 1 pg, judged from a criterion of a signal-to-noise ratio of 10. The precision and accuracy of the method were satisfactory.
Shimizu
K
K
Division of Biochemistry, Tottori University School of Medicine, Yonago, Japan.
Hara
T
T
Yamaga
N
N
Kohara
H
H
Nojima
K
K
D019326:17-alpha-Hydroxyprogesterone; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D006908:Hydroxyprogesterones / Q000097:blood*; D007202:Indicators and Reagents; D007553:Isotope Labeling; D014297:Trimethylsilyl Compounds / Q000097:blood / Q000138:chemical synthesis
D006908:Hydroxyprogesterones; D007202:Indicators and Reagents; D014297:Trimethylsilyl Compounds; D019326:17-alpha-Hydroxyprogesterone