Establishment and characterization of a murine Gardner's osteosarcoma cell line (GOS/T): purification of bone-specific alkaline phosphatase (ALPase) and use of anti-ALPase as a diagnostic acid.
Gardner's murine osteosarcoma has been successfully cultured and maintained as a clonal cell line (GOS/T), capable of forming a tumor with neoplastic bone and osteoid tissue by inoculation of 1 x 10(7) cultured cells. Alkaline phosphatase (ALPase) level in the culture medium and the serum from the tumor-bearing mice increased significantly three times and 16 to 105 times, respectively. The bone-specific ALPase was purified by butanol extraction following gel-filtration through Sephadex G-200. The molecular weight of ALPase was determined as 420,000, which was three times 140,000, the molecular weight of a subunit. Immunofluorescence staining using anti-ALPase antiserum, which was made by inoculation of partially purified ALPase, revealed a positive reaction for GOS/T cell line and osteoblasts of the fetal mice. The data presented here suggest that the cell line of GOS/T can be regarded as an established cell line, and the immunological reaction with anti-ALPase antibody is a useful model system to study human osteosarcoma.
Yumoto
T
T
Department of Pathology, Tottori University School of Medicine, Japan.
Naniwa
S
S
Yoshida
H
H
Nahiro
J
J
Kasagi
N
N
Maeda
N
N
D000469:Alkaline Phosphatase / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D000818:Animals; D000906:Antibodies / Q000276:immunology; D001842:Bone and Bones / Q000201:enzymology*; D002460:Cell Line; D051379:Mice; D012516:Osteosarcoma / Q000201:enzymology* / Q000473:pathology; D014407:Tumor Cells, Cultured / Q000201:enzymology / Q000473:pathology
D000906:Antibodies; D000469:Alkaline Phosphatase