Tris (2,3-dibromopropyl) phosphate nephrotoxicity in the rat: histological and biochemical changes in renal components by 13C-NMR spectra.
Rats receiving a single oral dose of 286.8 mumol/kg tris(2,3-dibromopropyl)phosphate (Tris-BP) showed pyknosis of the renal tubular epithelial cells on Day 1, necrosis on Day 2, regeneration from Day 3 and large nuclei formation from Day 4. 13C-NMR spectra were applied to clarify changes of the renal low-molecular-weight components in the kidney injured by Tris-BP, and sialic acid and inositol were found as the desired marker components. The lesions produced by Tris-BP were characterized by changes in the renal components and enzyme activities. Increases in sialic acid content of the kidney were observed both on Day 1, suggesting destruction of the epithelial cell membrane, and on Day 5, suggesting regeneration accompanied by an increase of inositol content on Days 7 and 10. Renal activity of cytoplasmic enzyme, alanine aminopeptidase, was decreased on Days 1 and 5 and elevated on Days 7 and 10. Lactate dehydrogenase activity showed a tendency to decrease in the kidneys on Day 1 and to increase significantly on Days 2, 5, 6 and 7.
Fukuoka
M
M
Division of Biological Chemistry, National Institute of Hygienic Sciences, Tokyo, Japan.
Tanaka
A
A
Yamaha
T
T
Naito
K
K
Takada
K
K
Kobayashi
K
K
Tobe
M
M
D000818:Animals; D002250:Carbon Radioisotopes; D005411:Flame Retardants / Q000633:toxicity*; D006651:Histocytochemistry; D007294:Inositol / Q000378:metabolism; D007668:Kidney / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology; D007674:Kidney Diseases / Q000139:chemically induced* / Q000378:metabolism / Q000473:pathology; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000473:pathology; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D010755:Organophosphates / Q000633:toxicity*; D009943:Organophosphorus Compounds / Q000633:toxicity*; D051381:Rats; D011919:Rats, Inbred Strains; D012794:Sialic Acids / Q000378:metabolism
D002250:Carbon Radioisotopes; D005411:Flame Retardants; D010755:Organophosphates; D009943:Organophosphorus Compounds; D012794:Sialic Acids; C012085:tris(2,3-dibromopropyl)phosphate; D007294:Inositol