Porcine malignant hyperthermia carrier detection and chromosomal assignment using a linked probe.
In pigs, the gene for glucosephosphate isomerase (GPI) is linked to the halothane (HAL) gene which is responsible for malignant hyperthermia (MH). A single copy DNA probe, designated GPI8R, has been isolated from a pig genomic library using a porcine GPI cDNA probe. This probe detects, as was the case for the cDNA probe, a five allele polymorphism in SacI and PvuII digested pig DNA. Family studies show that this polymorphism is linked to the HAL locus and hence can be used in carrier detection. In situ hybridization with GPI8R assigned the GPI locus to bands p12-q22 of chromosome 6. We conclude that the HAL linkage group resides on chromosome 6.
Davies
W
W
Department of Biochemistry, Norwegian College of Veterinary Medicine, Oslo.
Harbitz
I
I
Fries
R
R
Stranzinger
G
G
Hauge
J G
JG
D000818:Animals; D002874:Chromosome Mapping; D015342:DNA Probes; D004351:Drug Resistance / Q000235:genetics; D005260:Female; D006580:Genetic Carrier Screening; D008040:Genetic Linkage; D005956:Glucose-6-Phosphate Isomerase / Q000235:genetics; D006221:Halothane / Q000494:pharmacology; D008297:Male; D008305:Malignant Hyperthermia / Q000235:genetics / Q000662:veterinary*; D010375:Pedigree; D011110:Polymorphism, Genetic; D013552:Swine; D013553:Swine Diseases / Q000235:genetics*
D015342:DNA Probes; D005956:Glucose-6-Phosphate Isomerase; D006221:Halothane