Histochemical characteristics of glycoproteins in the bile duct system of mice immunized with swine serum.
The bile duct system of BALB/c and DDY mice, which were immunized with swine serum (SS) or not, was examined histochemically. Biliary epithelial cells of the SS-treated BALB/c mice, which were positively stained with periodic acid-Schiff (PAS) and had binding sites of Dolichos biflorus (DBA), were thought to secrete neutral glycoproteins with terminal N-acetyl-D-galactosamine residues. Those of the SS-treated DDY mice were however negatively or weakly stained with any histochemical stainings. On the other hand, glandular epithelial cells of the SS-treated mice of both strains, which were positively stained with high iron diamine-alcian blue (HID-AB) and had binding sites of DBA, Griffonia simplicifolia-II (GS-II), Ulex europaeus-I (UEA-I), and Triticum vulgaris (WGA), were thought to secrete glycoproteins with terminal sialic acid residues. Biliary and glandular epithelial cells of the normal mice contained only a small amount of glycoproteins showing similar histochemical characteristics to those in the SS-treated BALB/c mice. BALB/c mice immunized with SS were thought to be very useful for the investigation of production and secretion of glycoproteins in the bile duct system as well as being good model of bile duct disease.
Itagaki
S
S
Department of Biomedical Science, Faculty of Agriculture, University of Tokyo, Japan.
Honjo
K
K
Doi
K
K
Mitsuoka
T
T
D000818:Animals; D001652:Bile Ducts / Q000166:cytology / Q000276:immunology*; D001798:Blood Proteins / Q000276:immunology*; D004721:Endoplasmic Reticulum / Q000378:metabolism; D005704:Gallbladder / Q000166:cytology / Q000276:immunology; D006023:Glycoproteins / Q000276:immunology*; D006651:Histocytochemistry; D007114:Immunization; D037102:Lectins; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D037121:Plant Lectins; D013552:Swine
D001798:Blood Proteins; D006023:Glycoproteins; D037102:Lectins; D037121:Plant Lectins; C036554:dolichos biflorus agglutinin