Assays of serum laminin and type III procollagen peptide for monitoring the clinical course of diabetic microangiopathy.
Thickening of the capillary basement membrane is a characteristic feature of diabetes and is considered to cause diabetic microangiopathy. Serum levels of both laminin, a glycoprotein in the basement membrane, and type III procollagen peptide (P-III-P) were measured by specific radioimmunoassays according to the methods of Brocks et al. and Rohde et al. respectively and analyzed with regard to diabetic microangiopathy, glycemic control, diabetic duration and treatment. As a result, serum levels of both laminin and P-III-P showed higher values with development of diabetic microangiopathy, suggesting that progressive changes in diabetic microangiopathy occur with synthesis of laminin and P-III-P. Serum laminin and P-III-P appear to be good non-invasive markers for measuring basement membrane metabolism and type III collagen accumulation. High values of serum laminin and P-III-P levels were suspected to be due to improper treatment, using hypoglycemic agents without adherence to the diet regimen.
Okazaki
R
R
Department of Internal Medicine, Saiseikai Central Hospital, Tokyo, Japan.
Matsuoka
K
K
Horiuchi
A
A
Maruyama
K
K
Okazaki
I
I
D015415:Biomarkers / Q000097:blood*; D001786:Blood Glucose / Q000032:analysis; D003920:Diabetes Mellitus / Q000097:blood; D003925:Diabetic Angiopathies / Q000097:blood / Q000503:physiopathology*; D003928:Diabetic Nephropathies / Q000097:blood / Q000503:physiopathology; D003929:Diabetic Neuropathies / Q000097:blood / Q000503:physiopathology; D003930:Diabetic Retinopathy / Q000097:blood / Q000503:physiopathology; D006442:Glycated Hemoglobin / Q000032:analysis; D006801:Humans; D007797:Laminin / Q000097:blood*; D009765:Obesity; D010446:Peptide Fragments / Q000097:blood*; D011347:Procollagen / Q000097:blood*
D015415:Biomarkers; D001786:Blood Glucose; D006442:Glycated Hemoglobin; D007797:Laminin; D010446:Peptide Fragments; D011347:Procollagen; C041876:procollagen Type III-N-terminal peptide