The effect of isatin on the Echinococcus granulosus cyst in an experimental host.
The effect of isatin on the E. granulosus cyst was studied. NMRI mice, which were infected with E. granulosus of sheep origin, were treated daily with isatin at a dose of 50 mg kg-1 for 18 days. Ultrastructural damage was observed in the treated cysts, including accumulation of lamellar stacks, electron-dense granules, autophagosomes and lipid vesicles. Moreover, a biochemical study showed an inhibition of alkaline phosphatase activity, with a decrease in carbohydrate storage and an increase in acid phosphatase activity. In spite of the short duration of the treatment, the results obtained allowed us to conclude that isatin acts on E. granulosus cysts. This activity appears as a process of degeneration linked to the alkaline phosphatase inhibitory effect of isatin.
Piens
M A
MA
Departement de Parasitologie et Pathologie Exotique, Université Claude Bernard, Lyon, France.
Sarciron
E
E
Audin
P
P
Mojon
M
M
Petavy
A F
AF
Gabrion
C
C
D000135:Acid Phosphatase / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000037:antagonists & inhibitors; D000818:Animals; D004443:Echinococcosis / Q000188:drug therapy* / Q000469:parasitology; D004446:Echinococcus / Q000187:drug effects / Q000201:enzymology / Q000648:ultrastructure*; D005947:Glucose / Q000378:metabolism; D007211:Indoles / Q000627:therapeutic use*; D007510:Isatin / Q000494:pharmacology / Q000627:therapeutic use*; D008099:Liver / Q000201:enzymology / Q000378:metabolism; D051379:Mice; D008854:Microscopy, Electron
D007211:Indoles; D007510:Isatin; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D005947:Glucose