title: Release of D-[3H]aspartate and [14C]GABA in rat hippocampus slices: effects of fatty acid-free bovine serum albumin and Ca2+ withdrawal.
abstract: Extended incubation of hippocampus slices in the presence of fatty acid-free bovine serum albumin (FAF-BSA) strongly enhanced the release of D-[3H]aspartate and [14C]GABA induced by veratridine. Saturation of the FAF-BSA with oleic acid abolished the enhancing effect. Spontaneous release and K+-induced release were not significantly changed by the addition of FAF-BSA. Amino-oxyacetic acid in the medium enhanced the veratridine-induced release of D-[3H] aspartate. The spontaneous release of [14C]GABA was greatly increased by Ca2+ withdrawal. With the further addition of EGTA the spontaneous release in the absence of Ca2+ increased more than 8-fold over the measured in the presence of 1.5 mM Ca2+. The enhanced release caused by Ca2+ withdrawal was totally blocked by tetrodotoxin. The toxin was effective even when added after the spontaneous release in the absence of Ca2+ was already proceeding at a high rate. The veratridine-induced release of [14C]GABA was also considerably augmented by Ca2+ withdrawal. D-[3H]aspartate release, studied simultaneously with [14C]GABA by double labeling, did not show enhanced spontaneous release upon Ca2+ withdrawal. The findings provide evidence that the enhanced [14C]GABA release caused by Ca2+ withdrawal is mediated by voltage-dependent Na+ channels.
authors.affiliation: Department of Biological Chemistry, Hebrew University of Jerusalem, Israel.
authors.forename: D
authors.initials: D
authors.lastname: Minc-Golomb
authors.forename: S
authors.initials: S
authors.lastname: Eimerl
authors.forename: Y
authors.initials: Y
authors.lastname: Levy
authors.forename: M
authors.initials: M
authors.lastname: Schramm
mesh_terms: D000818:Animals; D001224:Aspartic Acid / Q000378:metabolism*; D002118:Calcium / Q000494:pharmacology*; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D051381:Rats; D012710:Serum Albumin, Bovine / Q000494:pharmacology*; D014701:Veratridine / Q000494:pharmacology; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
chemical_list: D012710:Serum Albumin, Bovine; D001224:Aspartic Acid; D005680:gamma-Aminobutyric Acid; D014701:Veratridine; D002118:Calcium