Determination of warfarin in human plasma by high performance liquid chromatography.
A simple procedure for the determination of warfarin in plasma samples is described. The assay involves a single ethereal extraction of warfarin and its internal standard, propyl paraben, from an acidified sample. The extract is evaporated to dryness at 45 degrees C and the residue is redissolved in methanol (50 microliters). A 25-microliters aliquot is injected into the liquid chromatograph and eluted with acetonitrile - 10 mM phosphate buffer at pH 3.5 (48:52, v/v) on a C8 pre-column linked to a 5-microns C8 reversed-phase column. The flow rate was set at 1.5 ml min-1 and UV detector at 215 nm. The method has been used to determine plasma concentrations of warfarin in Chinese patients with recent atrial fibrillation scheduled for electrocardioversion.
Chan
K
K
Department of Pharmacology, Chinese University of Hong Kong, Shatin, N.T.
Woo
K S
KS
D000284:Administration, Oral; D002681:China; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D013056:Spectrophotometry, Ultraviolet; D014859:Warfarin / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics
D014859:Warfarin