title: Bacteria-lectin interactions in phytohemagglutinin-induced bacterial overgrowth of the small intestine.
abstract: The mechanism of phytohemagglutinin-induced bacterial overgrowth of the small bowel in the rat was studied. Interaction of the lectin with bacterial isolates selected at random from those that comprised the major population of the overgrowth was determined. In both bacterial agglutination assays and glycocalyx stabilization, no specific association between lectin and bacteria was seen. In three independent binding assays phytohemagglutinin was not found to increase bacterial adherence to washed intestinal mucosa. Phytohemagglutinin would not appear to act, therefore, as a direct ligand to mediate bacterial adherence or to modify the mucosal surface to increase bacterial adherence.
authors.affiliation: Department of Biological Sciences, University of Calgary, Alta., Canada.
authors.forename: H
authors.initials: H
authors.lastname: Ceri
authors.forename: K
authors.initials: K
authors.lastname: Falkenberg-Anderson
authors.forename: R X
authors.initials: RX
authors.lastname: Fang
authors.forename: J W
authors.initials: JW
authors.lastname: Costerton
authors.forename: B
authors.initials: B
authors.lastname: Howard
authors.forename: J G
authors.initials: JG
authors.lastname: Banwell
mesh_terms: D000372:Agglutination Tests; D000818:Animals; D001419:Bacteria / Q000254:growth & development / Q000378:metabolism*; D001422:Bacterial Adhesion; D006023:Glycoproteins / Q000378:metabolism; D007413:Intestinal Mucosa / Q000382:microbiology; D007421:Intestine, Small / Q000382:microbiology*; D008024:Ligands; D010835:Phytohemagglutinins / Q000378:metabolism*; D011134:Polysaccharides / Q000378:metabolism; D051381:Rats
chemical_list: D006023:Glycoproteins; D008024:Ligands; D010835:Phytohemagglutinins; D011134:Polysaccharides