title: Development and degeneration of retina in rds mutant mice: analysis of interphotoreceptor matrix staining in chimaeric retina.
abstract: Chimaeric mice were produced by aggregating two morulae--one of homozygous rds mutant and another from a strain of mice with normal retina, which also differed in colour genes. The interphotoreceptor matrix in the retinal sections of these chimaeras was studied histochemically. In sections, stained with colloidal iron, regions with rds/rds photoreceptor layer, characteristically lacking the outer segments, showed more intense staining of the interphotoreceptor matrix, while regions with normal receptor outer segments showed less intense staining of the matrix. In sections, stained with toluidine blue, rds/rds regions showed more intense reaction along the pigment epithelial--photoreceptor interface and less intense reaction over the inner segments in comparison to the regions with normal photoreceptors. These differential staining reactions were independent of the overlying retinal pigment epithelial cell genotype and resembled the reaction patterns in the retina of pure strain controls of the same age. Small patches, showing rds/rds type staining were also observed within areas which appeared normal. We suggest that the altered properties of the interphotoreceptor matrix in the rds retina result from gene expression within the photoreceptor cells.
authors.affiliation: Department of Anatomy, Faculty of Medicine, Erasmus University, Rotterdam, The Netherlands.
authors.forename: S
authors.initials: S
authors.lastname: Sanyal
authors.forename: R K
authors.initials: RK
authors.lastname: Hawkins
authors.forename: G H
authors.initials: GH
authors.lastname: Zeilmaker
mesh_terms: D000818:Animals; D002678:Chimera; D005109:Extracellular Matrix / Q000473:pathology; D005260:Female; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008817:Mice, Mutant Strains; D010786:Photoreceptor Cells / Q000473:pathology; D012160:Retina / Q000473:pathology*; D012162:Retinal Degeneration / Q000235:genetics / Q000473:pathology*