title: Cloning and sequencing of a cDNA encoding the small subunit precursor of ribulose-1,5-bisphosphate carboxylase from Chlamydomonas moewusii. Evolution of RUBISCO SS polypeptide.
abstract: We have isolated and characterized a full-length cDNA clone encoding the precursor of the small subunit (pSU) of ribulose-1,5-bisphosphate carboxylase/oxygenase (RUBISCO) from the green alga, Chlamydomonas moewusii. Comparison with the C. reinhardtii rbcS1 gene sequence reveals that both small subunit (SS) coding regions are 75% homologous and that their predicted mature polypeptide chains are each composed of 140 amino acids. In contrast, their transit peptides appear to be divergent. We also show that transcription of the C. moewusii rbcS gene(s) which generates a 1,230 and a 930 base mRNA species are light-stimulated/or accumulated during the light period of the cell cycle. Finally, the SS polypeptide sequences of fifteen different photosynthetic organisms are compared; this analysis reveals at least five well-conserved polypeptide domains.
authors.affiliation: Département de biochimie, Faculté des sciences et de génie, Université Laval, Québec, Canada.
authors.forename: C
authors.initials: C
authors.lastname: Simard
authors.forename: C
authors.initials: C
authors.lastname: Lemieux
authors.forename: G
authors.initials: G
authors.lastname: Bellemare
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D005075:Biological Evolution; D002696:Chlamydomonas / Q000201:enzymology / Q000235:genetics*; D003001:Cloning, Molecular; D003062:Codon; D004247:DNA / Q000235:genetics; D004792:Enzyme Precursors / Q000235:genetics*; D005796:Genes; D008969:Molecular Sequence Data; D010944:Plants / Q000235:genetics; D015183:Restriction Mapping; D012273:Ribulose-Bisphosphate Carboxylase / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D014158:Transcription, Genetic
chemical_list: D003062:Codon; D004792:Enzyme Precursors; D004247:DNA; D012273:Ribulose-Bisphosphate Carboxylase