Antenatal diagnosis of neural tube defects using a coated bead immunoassay for acetylcholinesterase in amniotic fluid.
The development and validation of a coated bead immunoassay for amniotic fluid acetylcholinesterase is outlined. The assay has good precision (between assay CV of 6.8% within the normal range), and is linear up to 250 arbitrary units/L. The clinical validity of this assay has been assessed using a panel of amniotic fluid samples from normal and abnormal pregnancies. At an assay cut off level of 200 arbitrary units/L, all cases of neural tube defect-affected pregnancies were identified and the number of false positives was very small. False positives resulted from severe blood staining of the amniotic fluid. Since the monoclonal antibody used recognises red cell membrane acetylcholinesterase and the stored amniotic fluids had been frozen and thawed a number of times, the extent of this problem needs to be further assessed using freshly collected samples. The performance of this assay was found to be superior to the differential inhibitor colorimetric method and close to that of the electrophoretic procedure. The quantitative nature of the assay and the independence from operator technique makes it a useful adjunct to the measurement of amniotic fluid AFP in the prenatal diagnosis of neural tube defects.
Mehta
S
S
Department of Biochemistry, Oldchurch Hospital, Romford, Essex, UK.
Spencer
K
K
D000110:Acetylcholinesterase / Q000032:analysis*; D000653:Amniotic Fluid / Q000201:enzymology*; D000936:Antigen-Antibody Complex; D015415:Biomarkers / Q000032:analysis*; D004796:Clinical Enzyme Tests; D005260:Female; D006801:Humans; D007118:Immunoassay / Q000379:methods; D009436:Neural Tube Defects / Q000175:diagnosis*; D011247:Pregnancy; D011296:Prenatal Diagnosis
D000936:Antigen-Antibody Complex; D015415:Biomarkers; D000110:Acetylcholinesterase