Inhibition of expression of natural UAG suppressor glutamine tRNA in HIV-infected human H9 cells in vitro by Avarol.
HTLV-IIIB-infected H9 cells are shown to contain a high level of the natural UAG suppressor glutamine tRNA(UmUG Gln); this tRNA has been demonstrated to be required for the synthesis of Moloney murine leukemia virus (Mo-MuLV)-encoded protease. After cultivation of HTLV-IIIB-infected H9 cells with Avarol at a concentration (1 microgram/ml), previously found to protect the cells against the cytopathic effects of HTLV-III, an almost complete inhibition of the synthesis of the tRNA(UmUG Gln) was observed. Moreover, we obtained some evidence that the processing of the HTLV-III precursor protein p53 to p24 is inhibited by Avarol in infected cells, suggesting that the compound interferes with the expression of the viral protease gene.
Müller
W E
WE
Abt. Angewandte Molekularbiologie, Universität Mainz, West Germany.
Schröder
H C
HC
Reuter
P
P
Sarin
P S
PS
Hess
G
G
Meyer zum Büschenfelde
K H
KH
Kuchino
Y
Y
Nishimura
S
S
D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D002460:Cell Line; D002472:Cell Transformation, Viral / Q000187:drug effects*; D003062:Codon; D005973:Glutamine / Q000378:metabolism; D006678:HIV / Q000187:drug effects / Q000235:genetics*; D006801:Humans; D009693:Nucleic Acid Hybridization; D014176:Protein Biosynthesis; D012345:RNA, Transfer, Amino Acid-Specific / Q000235:genetics*; D012351:RNA, Transfer, Gln / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism; D012717:Sesquiterpenes / Q000494:pharmacology*; D013489:Suppression, Genetic / Q000187:drug effects*; D014027:Tobacco Mosaic Virus / Q000235:genetics
D000998:Antiviral Agents; D003062:Codon; D012345:RNA, Transfer, Amino Acid-Specific; D012351:RNA, Transfer, Gln; D012367:RNA, Viral; D012717:Sesquiterpenes; D005973:Glutamine; C023663:avarol