title: Applications of universal probe on DNA hybridization.
abstract: A convenient method for DNA hybridization termed "Universal probe" is described which is based on the principle of sandwich hybridization. This system consists of two probes: primary probe which is single-stranded DNA prepared from a chimeric phage-plasmid vector containing the complementary sequence to a target; and labeled secondary probe which has an opposite strand of the primary probe without the complementary sequence. By use this universal probe human beta-globin gene was able to be detected on Southern blots of genomic DNA. A potential advantage of this method is that the single-stranded primary probe is prepared easily by the chimeric phage-plasmid vector system and tedious labeling is not needed each time.
authors.affiliation: Central Research Laboratories, Wakunaga Pharmaceutical Co., Ltd., Hiroshima, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Yamane
authors.forename: S
authors.initials: S
authors.lastname: Nakagami
authors.forename: K
authors.initials: K
authors.lastname: Miyoshi
mesh_terms: D003001:Cloning, Molecular / Q000379:methods; D004247:DNA / Q000235:genetics*; D015342:DNA Probes; D009693:Nucleic Acid Hybridization; D010957:Plasmids
chemical_list: D015342:DNA Probes; D004247:DNA