Clearance of surfactant phosphatidylcholine via the upper airways in rabbits.
A possible route of clearance of surfactant phosphatidylcholine from the lungs is via the airways. To quantify surfactant loss via this pathway, latex bags were surgically placed into the abdomens of adult rabbits such that secretions cleared via the esophagus could be collected. The rabbits then were given treatment or trace doses of radiolabeled phosphatidylcholine-surfactant by tracheal injection and/or intravascular radiolabeled precursors of phosphatidylcholine. Labeled saturated phosphatidylcholine was measured in all fluids that were collected from the bags at 2-h intervals for 24 h and in alveolar washes and lung tissues at 24 h. No more than 7% of either treatment or trace doses of intratracheal surfactant-saturated phosphatidylcholine was lost via clearance up the airways over 24 h. Clearances of endogenously synthesized and secreted saturated phosphatidylcholine were estimated to be no more than 3% of the flux of labeled saturated phosphatidylcholine through the alveolar pool. These experiments demonstrate that surfactant phosphatidylcholine clearance via movement up the airways is not a major pathway leading to surfactant catabolism.
Pettenazzo
A
A
Department of Pediatrics, University of California, Los Angeles School of Medicine, Torrance 90509.
Jobe
A
A
Humme
J
J
Seidner
S
S
Ikegami
M
M
D000818:Animals; D008168:Lung / Q000378:metabolism; D008657:Metabolic Clearance Rate; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000378:metabolism; D010713:Phosphatidylcholines / Q000378:metabolism*; D011663:Pulmonary Surfactants / Q000378:metabolism*; D011817:Rabbits; D012137:Respiratory System / Q000378:metabolism*
D010169:Palmitic Acids; D010713:Phosphatidylcholines; D011663:Pulmonary Surfactants; D019308:Palmitic Acid