Efficient oligoadenylate synthesis catalyzed by uranyl ion complex in aqueous solution.
Polymerization of adenosine-5'-phosphorimidazolide in an aqueous solution was conducted with uranyl ion as a catalyst. Oligoadenylate formation took place efficiently with high regio-selectivity (2'-5' linkage). The oligoadenylates up to hexadecamer were obtained in a high total yield. The chain length and the regio-selectivity of the resulting oligoadenylates varied greatly depending on the concentration of the uranyl ion catalyst. The oligonucleotide formation is likely to be mediated by uranylnucleotide complex.
Sawai
H
H
Department of Chemistry, Faculty of Engineering, Gunma University, Japan.
Kuroda
K
K
Hojo
T
T
D000227:Adenine Nucleotides / Q000138:chemical synthesis* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D007202:Indicators and Reagents; D009843:Oligoribonucleotides / Q000138:chemical synthesis* / Q000302:isolation & purification; D012996:Solutions; D014501:Uranium
D000227:Adenine Nucleotides; D007202:Indicators and Reagents; D009843:Oligoribonucleotides; D012996:Solutions; D014501:Uranium; C023505:2',5'-oligoadenylate