title: Chromosome banding in direct preparations of chorionic villi.
abstract: Chorionic villus sampling (CVS) is now currently offered for first trimester prenatal diagnosis of genetic disorders. Chromosome analysis of CVS in direct and culture preparations is possible using modifications of standard banding techniques. We summarize our experience in applying QFQ, GTG, RBG, CBG, DA/DAPI, NOR, and SC differentiation protocols to direct preparations. Characteristic chromosome regions are properly labelled by these techniques, and analysis of 300 band stage karyotypes is consistently achievable on GTG banded direct preparations. However, banding of CVS direct chromosomes has proved to be difficult, and the analysis needs to be backed up by culture preparations.
authors.affiliation: Paediatric Research Unit, United Medical School, Guy's Hospital, London, U.K.
authors.forename: M
authors.initials: M
authors.lastname: Murer-Orlando
authors.forename: J
authors.initials: J
authors.lastname: Llerena
authors.forename: M
authors.initials: M
authors.lastname: McGuire
authors.forename: L
authors.initials: L
authors.lastname: Zahed
authors.forename: J
authors.initials: J
authors.lastname: Crolla
authors.forename: M
authors.initials: M
authors.lastname: Bobrow
authors.forename: R
authors.initials: R
authors.lastname: Sheridan
mesh_terms: D002824:Chorionic Villi / Q000648:ultrastructure*; D002871:Chromosome Banding / Q000379:methods*; D002875:Chromosomes / Q000032:analysis / Q000648:ultrastructure; D005260:Female; D006801:Humans; D011247:Pregnancy; D011296:Prenatal Diagnosis / Q000379:methods