Photocurrent measurements of the purple membrane oriented in a polyacrylamide gel.
When illuminated, oriented purple membranes isolated from Halobacterium halobium give a photoelectric effect. The frequency response of a photocurrent measuring system for purple membranes oriented and immobilized in a polyacrylamide gel is analyzed from DC to 100 MHz. The waveform of the photocurrent can depend on both the sample conditions (including bathing solution) and the measuring system (electrode and ammeter) at both the low and high frequency ends. In the DC-1 kHz range (millisecond signals), the apparent lifetime of the photocurrent component is distorted if the electrode is not platinized and if the conductivity of the bathing solution is not low. In the 1 kHz to 1 MHz range (microsecond signals), the frequency response is flat under most conditions. In the MHz range (nanosecond signals), the apparent lifetime of the photocurrent component will be distorted if the conductivity of the bathing solution is not high and if the input impedance of the ammeter is not low and constant throughout the frequency range. With our optimized apparatus, we could measure the photocurrent components from oriented purple membrane with lifetimes from 70 ms to 32 ns without distortion by the measuring system.
Liu
S Y
SY
University of Illinois, Department of Physiology and Biophysics, Urbana 61801.
Ebrey
T G
TG
D000178:Acrylamides; D001436:Bacteriorhodopsins / Q000378:metabolism* / Q000528:radiation effects; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods; D006217:Halobacterium; D007700:Kinetics; D008027:Light; D008564:Membrane Potentials
D000178:Acrylamides; D001436:Bacteriorhodopsins