Sebum-suppressing activity of the nonpolar arotinoid Ro 15-0778 in rodents.
Retinoids are known to modulate sebaceous gland activity in humans and animals. The nonpolar arotinoid Ro 15-0778 [(E)-1,2,3,4-tetrahydro-1,1,4,4-tetramethyl-6-(1-methyl-2-phenylethen yl) naphthalene] does not contain a polar end group and is devoid of the classical retinoid side effects of hypervitaminosis A. The favorable toxicological profile stimulated the evaluation of this arotinoid in animal models of sebum production. In castrated, testosterone-stimulated male rats, Ro 15-0778 is 50 times more potent than 13-cis-retinoic acid in inhibiting the production and subsequent secretion of sebum. The oral ED50 value of Ro 15-0778 is 30 micrograms/kg, while an oral dose of 0.5 mg/kg inhibited sebum secretion nearly 100%. In testosterone-stimulated female rats, Ro 15-0778 inhibits sebum secretion significantly with an oral ED50 of 140 micrograms/kg and an s.c. ED50 of 75 micrograms/kg. Ro 15-0778 was also evaluated for its ability to prevent testosterone induction of the immature hamster flank organ. The topical ED50 is 0.53 mg/kg and the oral ED50 is 38 mg/kg. This arotinoid is similarly active in mature male hamsters without testosterone treatment. In addition, the retinoid is active topically and orally in reducing the size of the gerbil abdominal sebaceous gland. The compound exhibits no antiandrogenic activity when tested in ventral prostrate and seminal vesicle assays in rats. Additionally, the compound does not have estrogenic activity when tested in the rat uterine weight assay. High doses of Ro 15-0778 in humans did not demonstrate significant sebum-suppressing activity.(ABSTRACT TRUNCATED AT 250 WORDS)
Boris
A
A
Department of Pharmacology and Chemotherapy, Hoffmann-La Roche, Nutley, NJ 07110.
Hurley
J
J
Wong
C Q
CQ
Comai
K
K
Shapiro
S
S
D000726:Androgen Antagonists; D000818:Animals; D006224:Cricetinae; D005260:Female; D005849:Gerbillinae; D008297:Male; D009929:Organ Size / Q000187:drug effects; D011467:Prostate / Q000033:anatomy & histology / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains; D012176:Retinoids / Q000008:administration & dosage / Q000494:pharmacology*; D012627:Sebaceous Glands / Q000033:anatomy & histology / Q000187:drug effects / Q000378:metabolism; D012629:Sebum / Q000187:drug effects* / Q000378:metabolism; D013739:Testosterone / Q000494:pharmacology
D000726:Androgen Antagonists; D012176:Retinoids; D013739:Testosterone; C053595:temarotene