title: The detection, pharmacokinetics and behavioral effects of diisopropylamine dichloroacetate (DADA) in the horse: a preliminary report.
abstract: 1. Drug administration studies using diisopropylamine dichloroacetate (DADA) and diisopropylamine (DIPA) were conducted in Thoroughbred and Standardbred horses to assess physiological effects and develop detection methods. 2. Four horses received 0.08 mg DADA/kg body wt and showed no changes in heart and respiratory rates or body temperature as measured over a 1-hr period after administration. A transient diuretic effect was found to occur in 2 mares dosed with 0.80 mg DADA/kg body wt. 3. A qualitative detection method using thin-layer chromatography was developed to detect DIPA, the major metabolite of DADA in equine urine. A quantitative detection method (lower limit of detection 0.5 micrograms/ml urine) for this metabolite was also developed using gas chromatography. 4. Neither DADA or the free base, DIPA, were detectable in equine blood samples using the above-mentioned methodologies.
authors.affiliation: Department of Veterinary Science, Maxwell H. Gluck Equine Research Center, University of Kentucky, Lexington 40546-0099.
authors.forename: J M
authors.initials: JM
authors.lastname: Yang
authors.forename: W E
authors.initials: WE
authors.lastname: Woods
authors.forename: T J
authors.initials: TJ
authors.lastname: Weckman
authors.forename: T W
authors.initials: TW
authors.lastname: Wood
authors.forename: S L
authors.initials: SL
authors.lastname: Chang
authors.forename: J W
authors.initials: JW
authors.lastname: Blake
authors.forename: T
authors.initials: T
authors.lastname: Tobin
mesh_terms: D000818:Animals; D000959:Antihypertensive Agents / Q000097:blood / Q000494:pharmacology* / Q000652:urine; D001522:Behavior, Animal / Q000187:drug effects*; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D005260:Female; D006736:Horses / Q000378:metabolism* / Q000502:physiology; D008297:Male; D013058:Mass Spectrometry; D000644:Quaternary Ammonium Compounds / Q000097:blood / Q000493:pharmacokinetics* / Q000652:urine; D012119:Respiration / Q000187:drug effects
chemical_list: D000959:Antihypertensive Agents; D000644:Quaternary Ammonium Compounds; C001002:diisopropylamine dichloroacetate