Bioluminescent monitoring of ATP release from human red blood cells treated with nonionic detergent.
The adaptation of the firefly luciferin-luciferase system for the detection of ATP in the presence of Triton X 100 or Brij 58 nonionic detergents is described. The method is sensitive, easy to perform and is suitable for the continuous monitoring of the mobilization rate of ATP from Triton X 100- or Brij 58-treated human erythrocytes. A very rapid ATP release was observed after Triton X 100 treatment of the red blood cells while the presence of Brij 58 caused a retarded mobilization of the intracellular ATP. The technique described seems to be applicable to all kinds of cells.
Köszegi
T
T
Department of Clinical Chemistry, University Medical School, Pécs, Hungary.
Kellermayer
M
M
Kövecs
F
F
Jobst
K
K
D000255:Adenosine Triphosphate / Q000097:blood / Q000378:metabolism*; D002592:Cetomacrogol; D003902:Detergents / Q000494:pharmacology*; D004912:Erythrocytes / Q000378:metabolism*; D006801:Humans; D008164:Luminescent Proteins; D017830:Octoxynol; D011092:Polyethylene Glycols; D013501:Surface-Active Agents / Q000494:pharmacology*
D003902:Detergents; D008164:Luminescent Proteins; D013501:Surface-Active Agents; D011092:Polyethylene Glycols; D000255:Adenosine Triphosphate; D017830:Octoxynol; D002592:Cetomacrogol