Interaction of DNA hairpin loops and a complementary strand by a triplet of base pairs.
Hypothesis of non-enzymatic recognition of primordial tRNA and mRNA precursors is experimentally approached. DNA hairpins containing a different number of deoxyguanosine residues in the loop are analyzed for their binding ability to a chemically fixed single-strand of oligo(dC). In presence of small Mg2+ concentration a hairpin with five dG residues in the loop is adsorbed to affinity matrix. Comparison of elution temperatures of hairpin oligonucleotides with those of single-stranded oligoguanylic acids with length of the loop indicates, that smallest loop able to bind forms a triplet of base pairs.
Baumann
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U
Max-Planck-Institut für Biophysikalische Chemie, Göttingen, FRG.
Frank
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R
Blöcker
H
H
D000327:Adsorption; D001482:Base Composition; D002846:Chromatography, Affinity; D004247:DNA / Q000378:metabolism*; D003849:Deoxyguanosine / Q000378:metabolism; D006150:Guanine Nucleotides; D008274:Magnesium / Q000494:pharmacology; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000378:metabolism; D009843:Oligoribonucleotides; D012322:RNA Precursors / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D012343:RNA, Transfer / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology; D013696:Temperature
D006150:Guanine Nucleotides; D009838:Oligodeoxyribonucleotides; D009843:Oligoribonucleotides; D012322:RNA Precursors; D012333:RNA, Messenger; C036895:oligo(G); C033539:oligodeoxycytidylic acid; C033538:oligodeoxyguanylic acid; D012965:Sodium Chloride; D004247:DNA; D012343:RNA, Transfer; D003849:Deoxyguanosine; D008274:Magnesium