[The viability of rat amd mouse embryos in low-temperature preservation].
Embryos of the laboratory rats and mice at the two-cell stage were frozen in different media with 1.5 M DMSO at a rate of 0.5-0.7 degrees/min to -40 degrees and the transferred into liquid nitrogen. Temperature of the crystallization initiation was -6 degrees. After thawed embryos were transplanted to foster mothers, these latter gave birth to normal offspring. The best results (38% of the total number of transplanted embryos in mice and 55% in rats) were obtained when using modified Whitten's medium for cryopreservation. Survival of the rat embryos in the NT-6 medium fell to 36% due, supposedly, to changes in pH accompanying the decrease in temperature. These results allow us to recommend modified Whitten's medium and the developed conditions of freezing-thawing for cryopreservation of the rat and mouse embryos.
Mezhevikina
L M
LM
Ignat'eva
T V
TV
Veprintsev
B N
BN
D000818:Animals; D003470:Culture Media; D004624:Embryo Transfer; D004622:Embryo, Mammalian / Q000502:physiology*; D005260:Female; D005615:Freezing; D006863:Hydrogen-Ion Concentration; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D051381:Rats; D013696:Temperature; D014021:Tissue Preservation / Q000379:methods*; D014022:Tissue Survival
D003470:Culture Media
Vyzhivaemost' émbrionov krys i mysheĭ pri nizkotemperaturnoĭ konservatsii.