Amplification of human minisatellites by the polymerase chain reaction: towards DNA fingerprinting of single cells.
Hypervariable minisatellites can be amplified from human DNA by the polymerase chain reaction, using primers from DNA flanking the minisatellite to amplify the entire block of tandem repeat units. Minisatellite alleles up to 5-10 kb long can be faithfully amplified. At least six minisatellite loci can be co-amplified from the same DNA sample and simultaneously detected to provide a reproducible and highly variable DNA fingerprint which can be obtained from nanogram quantities of human DNA. The polymerase chain reaction can also be used to analyse single target minisatellite molecules and single human cells, despite the appearance of spurious PCR products from some hypervariable loci. DNA fingerprinting at the level of one or a few cells therefore appears possible.
Jeffreys
A J
AJ
Department of Genetics, University of Leicester, UK.
Wilson
V
V
Neumann
R
R
Keyte
J
J
D000483:Alleles; D015139:Blotting, Southern; D004276:DNA, Satellite / Q000302:isolation & purification / Q000378:metabolism*; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D005784:Gene Amplification; D005838:Genotype; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes
D004276:DNA, Satellite; D015345:Oligonucleotide Probes; D004259:DNA-Directed DNA Polymerase