Transforming activities of sodium fluoride in cultured Syrian hamster embryo and BALB/3T3 cells.
The transforming activity of sodium fluoride was studied in the SHE and the BALB/3T3 cell culture systems. Initiating and promoting activities were then investigated by means of the orthogonal methodology. Sodium fluoride was found to induce morphological transformation of SHE cells seeded on a feeder layer of X-irradiated cells at high concentrations (75-125 micrograms/ml). When the cells were seeded in the absence of a feeder-layer, the transformation frequencies increased in a dose-dependent manner with the concentrations of sodium fluoride ranging from 0 to the highly toxic concentration of 200 micrograms/ml. In the BALB/3T3 cell system, sodium fluoride was negative in the standard Kakunaga procedure, while through the experiment designed by table L8 (2(7] of the orthogonal method, an initiating-like effect and a weak promoting activity were detected within the concentrations ranging from a 25 micrograms/ml to a 50 micrograms/ml concentration which is highly toxic for BALB/3T3 cells. From these results, it is suggested that, besides a genetic mode of action, sodium fluoride could possibly act through a non-genotoxic mechanism.
Lasne
C
C
Institut de Recherches Scientifiques sur le Cancer, Villejuif, France.
Lu
Y P
YP
Chouroulinkov
I
I
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D002478:Cells, Cultured; D006224:Cricetinae; D004305:Dose-Response Relationship, Drug; D008647:Mesocricetus; D051379:Mice; D008807:Mice, Inbred BALB C; D012969:Sodium Fluoride / Q000633:toxicity*
D012969:Sodium Fluoride