title: Effects of trembler mouse serum and laminin on oligodendrocyte proliferation and differentiation in culture.
abstract: Oligodendrocytes in primary cultures derived from rat embryo spinal cord were examined in control medium and in Trembler mouse serum (TMS)-supplemented medium. The oligodendrocytes were identified on the basis of the synthesis and surface expression of galactocerebrosides revealed by a monoclonal antibody directed against this component. We noticed two effects of TMS compared to control mouse serum. First, our results revealed that in TMS medium there is a mitogenic response of galactocerebroside (GalC)-positive cells. Second, in the presence of TMS, oligodendrocytes do not develop processes as they do in the presence of normal mouse serum. When laminin, a basal lamina component was added to TMS medium, GalC+ oligodendrocytes decreased in number and differentiation was normal. Possible explanations of the effects of TMS and laminin on oligodendrocyte proliferation and differentiation are discussed.
authors.affiliation: INSERM U.153, Paris, France.
authors.forename: D
authors.initials: D
authors.lastname: Hantaz-Ambroise
authors.forename: M
authors.initials: M
authors.lastname: Vigny
authors.forename: F
authors.initials: F
authors.lastname: Alliot
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D007797:Laminin / Q000494:pharmacology*; D051379:Mice; D008818:Mice, Neurologic Mutants / Q000097:blood*; D009457:Neuroglia / Q000502:physiology*; D009836:Oligodendroglia / Q000187:drug effects / Q000502:physiology*; D013116:Spinal Cord / Q000166:cytology / Q000196:embryology*
chemical_list: D007797:Laminin