A new chromogenic assay for direct detection of staphylocoagulase.
Tube test for detection of staphylococcal coagulase, despite of many disadvantages, is commonly used in clinical microbiology for identification of Staphylococcus aureus. In this paper a new chromogenic method for detection of the coagulase directly in staphylococcal cultures is described and evaluated on the basis of a comparison with the standard tube assay. The chromogenic assay appeared to be as sensitive as the tube test but results of the former one can be read in a few hours without any apparatus.
Bulanda
M
M
Institute of Microbiology, Medial Academy, Kraków, Poland.
Wegrzynowicz
Z
Z
Ciurak
M
M
Kowalczyk
J
J
Kupryszewski
G
G
Gruszka
M
M
Heczko
P B
PB
Pulverer
G
G
D002863:Chromogenic Compounds; D003030:Coagulase / Q000032:analysis*; D003124:Colorimetry; D003470:Culture Media; D006801:Humans; D011237:Predictive Value of Tests; D013211:Staphylococcus aureus / Q000201:enzymology*
D002863:Chromogenic Compounds; D003030:Coagulase; D003470:Culture Media