Effects of Tokishakuyakusan and its components on rat corpus luteum function in vitro.
27-day-old female rats received 20 IU PMS and 56 hours later, 40 IU hCG. Seven days after hCG treatment, the resected ovaries were perifused with NIH-LH, Tokishakuyakusan (TJ-23) or its components. LH and TJ-23 increased progesterone in media and LH was more effective than TJ-23. Hoelen, Peony root, Alisma rhizome and Japanese angelica root also augmented progesterone. 17 alpha-hydroxyprogesterone and 20 alpha-hydroxyprogesterone were not stimulated by TJ-23. These results suggest that TJ-23 stimulates corpora lutea to secrete progesterone without direct luteotropic or luteolytic effect and that the effect may be attributable to Hoelen, Peony root, Alisma rhizome or Japanese angelica root.
Usuki
S
S
Department of Obstetrics and Gynecology, University of Tsukuba, Ibaraki, Japan.
D000818:Animals; D003338:Corpus Luteum / Q000187:drug effects* / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D006027:Glycosides / Q000494:pharmacology*; D006908:Hydroxyprogesterones / Q000737:chemistry; D007986:Luteinizing Hormone / Q000494:pharmacology; D010946:Plants, Medicinal; D011374:Progesterone / Q000378:metabolism; D051381:Rats
D004365:Drugs, Chinese Herbal; D006027:Glycosides; D006908:Hydroxyprogesterones; C413220:toki-shakuyaku-san; D011374:Progesterone; D007986:Luteinizing Hormone