Acute and chronic effects of the neurolytic agent ricin on dorsal root ganglia, spinal cord, and nerves.
The short- and long-term effects of ricin injections into nerves have been evaluated with light microscopy in the dorsal root ganglia, spinal cord, and peripheral nerves in rats and cats. Dorsal root ganglion cells initially exhibited chromatolysis, followed by gliosis and cell death. These changes were associated with Fink-Heimer degeneration in the somatotopically appropriate region of the dorsal horn. There were no signs of chromatolysis in dorsal horn neurons in ricin-injected animals, but chromatolytic motoneurons were observed. Ricin produced acute necrosis of injected nerves and dissolution of axoplasm. At long survival times (greater than 4 weeks) some apparently regenerating axons were seen in the injection sites of rats. Cell counts indicated that a substantial percentage of dorsal root ganglion neurons associated with the injected nerves were killed, but the presence of regenerating axons suggested that some cells survived the ricin treatment. Although the lesion may not always be complete, even with maximum sublethal doses, this method appears to be useful for specifically destroying afferent fibers associated with a particular nerve without transynaptic destruction of dorsal horn neurons.
Pubols
L M
LM
Neurological Sciences Institute, Good Samaritan Hospital and Medical Center, Portland, Oregon 97209.
Foglesong
M E
ME
D000818:Animals; D002415:Cats; D005260:Female; D005727:Ganglia, Spinal / Q000187:drug effects / Q000473:pathology*; D008297:Male; D009416:Nerve Regeneration; D009498:Neurotoxins; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values; D012276:Ricin / Q000633:toxicity*; D012584:Sciatic Nerve / Q000187:drug effects / Q000473:pathology*; D013116:Spinal Cord / Q000187:drug effects / Q000473:pathology*
D009498:Neurotoxins; D012276:Ricin