title: Characterization of an antigenically related family of cell-type specific proteins implicated in slug migration in Dictyostelium discoideum.
abstract: The monoclonal antibody MUD50 recognizes a group of developmentally regulated proteins, which are almost exclusively expressed by prespore cells in developing aggregates of Dictyostelium discoideum. Some of these antigens are integrally associated with the cell membrane, as assessed by physical and detergent-fractionation procedures. The MUD50-reactive proteins are glycosylated and some are phosphorylated. Post-translational modification is the common antigenic feature that is recognized by the MUD50 antibody in these cell-type-specific proteins. A glycosylation-defective mutant, DL118, (modB) does not express the MUD50 epitope, but does express the MUD52 epitope, which is found on a different group of glycoproteins. Therefore, we conclude that MUD50 recognizes a particular carbohydrate epitope on a restricted group of proteins. These proteins are structurally diverse, but are apparently involved in the maintenance of structure and movement of the multicellular D. discoideum slug.
authors.affiliation: Department of Molecular Biology, Research Institute of Scripps Clinic, La Jolla, California 92037.
authors.forename: S
authors.initials: S
authors.lastname: Alexander
authors.forename: E
authors.initials: E
authors.lastname: Smith
authors.forename: L
authors.initials: L
authors.lastname: Davis
authors.forename: A
authors.initials: A
authors.lastname: Gooley
authors.forename: S B
authors.initials: SB
authors.lastname: Por
authors.forename: L
authors.initials: L
authors.lastname: Browne
authors.forename: K L
authors.initials: KL
authors.lastname: Williams
mesh_terms: D000911:Antibodies, Monoclonal; D002465:Cell Movement; D004023:Dictyostelium / Q000276:immunology* / Q000502:physiology; D005786:Gene Expression Regulation; D006023:Glycoproteins / Q000276:immunology* / Q000502:physiology; D008970:Molecular Weight; D010750:Phosphoproteins / Q000276:immunology / Q000502:physiology; D011499:Protein Processing, Post-Translational
chemical_list: D000911:Antibodies, Monoclonal; D006023:Glycoproteins; D010750:Phosphoproteins