In vivo effects of doxorubicin and isoproterenol on reduced glutathione and H2O2 production in mouse heart.
Some parameters of free radical generation were studied in the hearts of CD 1 mice at short time intervals after the administration of Doxorubicin (15 mg/Kg i.v.) or Isoproterenol (80 mg/Kg s.c.). The two drugs consistently caused a decrease in cardiac reduced glutathione. Isoproterenol significantly increased in vivo H2O2 production by the heart at all the intervals taken into consideration, i.e., 5, 8, 12 and 16 hours after its administration. However, Doxorubicin did not significantly modify H2O2 generation at the same hours. These findings suggest that an increase in H2O2 production may not be involved in the oxidative stress caused by the anthracyclines at cardiac level.
D'Alessandro
N
N
Istituto di Farmacologia, Messina, Italy.
Rausa
L
L
Crescimanno
M
M
D000640:Amitrole / Q000494:pharmacology; D000818:Animals; D004317:Doxorubicin / Q000494:pharmacology*; D005260:Female; D005978:Glutathione / Q000378:metabolism*; D006321:Heart / Q000187:drug effects*; D006861:Hydrogen Peroxide / Q000378:metabolism*; D007545:Isoproterenol / Q000494:pharmacology*; D051379:Mice; D009206:Myocardium / Q000378:metabolism; D013997:Time Factors
D004317:Doxorubicin; D006861:Hydrogen Peroxide; D005978:Glutathione; D007545:Isoproterenol; D000640:Amitrole