Antigenic contraction of guinea pig tracheal preparations passively sensitized with monoclonal IgE: pharmacological modulation.
Spirally cut guinea pig tracheal preparations were passively sensitized using a mouse monoclonal IgE antibody against dinitrophenol (DNP). Maximal contraction observed following challenge with DNP-bovine serum albumin (DNP-BSA, 5 micrograms/ml; n = 20) response was approximately 46% of the histamine response (0.52 +/- 0.09 g/mm2; n = 53). Indomethacin (1.7 microM) increased and PGE2 (1 microM) decreased the response to the antigen. FPL 55712 (10 microM), atropine (0.1 microM), L 651392 (5 microM) or tripelennamine (1 microM) always reduced the maximal DNP-BSA response, but not BN 52021 (100 microM). This model may be used for rapid detection of compounds with antiallergic properties on IgE-dependent lung pathological states.
Haye-Legrand
I
I
INSERM U.200, Université Paris Sud, Clamart, France.
Norel
X
X
Labat
C
C
Benveniste
J
J
Brink
C
C
D000818:Animals; D000911:Antibodies, Monoclonal / Q000502:physiology*; D000941:Antigens / Q000276:immunology*; D001285:Atropine / Q000494:pharmacology; D002867:Chromones / Q000494:pharmacology; D004140:Dinitrophenols / Q000276:immunology*; D004306:Dose-Response Relationship, Immunologic; D006168:Guinea Pigs; D007116:Immunization, Passive; D007073:Immunoglobulin E / Q000502:physiology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009119:Muscle Contraction / Q000187:drug effects*; D010640:Phenothiazines / Q000494:pharmacology; D012710:Serum Albumin, Bovine / Q000276:immunology*; D014132:Trachea; D014309:Tripelennamine / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D000941:Antigens; D002867:Chromones; D004140:Dinitrophenols; D010640:Phenothiazines; C034638:dinitrophenyl-bovine serum albumin; D012710:Serum Albumin, Bovine; D007073:Immunoglobulin E; D014309:Tripelennamine; D001285:Atropine; C006312:FPL 55712; C053726:4-bromo-2,7-dimethoxy-3H-phenothiazin-3-one