RES-macrophage stimulation and liver tumour growth in rats; evaluation with dynamic liver RES scintigraphy.
Dynamic liver RES scintigraphy with Nanocoll (99Tcm albumin colloid - 50 nm diameter) assessing RES-macrophage phagocytic function was performed in 40 control, 73 RES-stimulated non-tumour-bearing and 59 tumour-bearing Wistar/FU rats in vivo. Tumour-bearing rats were inoculated with 10(6) x 1.0 cells of a syngeneic nitrosoguanidine-induced colonic carcinoma in the liver. Twenty-eight of these rats had been treated one day previously with Zymosan (3 mg x 100 g-1 i v) as a RES stimulant. The clearance/uptake rate (k) of Nanocoll was calculated from dynamic liver images by the slope in the plot 1n [1 - U(t)/U] versus t where t is time and U liver uptake. k-value in control animals was 0.45 +/- 0.01.10(-2) x s-1 Zymosan injection in non-tumour-bearing rats caused stati-stically significant higher clearance/uptake rate on day 1, through 8 after treatment compared to that of controls. On day 8 k-value was 0.64 +/- 0.04. In tumour-bearing rats the uptake rate (k) was on day 8 0.66 +/- 0.03, while in RES-stimulated tumour-bearing rats it was 0.64 +/- 0.03. Survival was 22 +/- 1 days in tumour bearing rats and 37 +/- 4 days in RES stimulated tumour-bearing rats. The average tumour volume after one week was 132 +/- 24 mm3 in non-stimulated rats and 20 +/- 5 mm3 in RES stimulated rats. There was a negative correlation between uptake rate and tumour volume and a positive correlation between uptake rate and survival on day 8 in non-stimulated tumour-bearing rats. Dynamic liver RES scintigraphy with small size 99Tcm albumin colloid (Nanocoll) can be used to measure RES phagocytic function and the effect of liver tumour growth on RES.
Holmberg
S B
SB
Department of Surgery, Sahlgrenska Hospital, Göteborg, Sweden.
Hafström
L
L
Jacobsson
L
L
D000230:Adenocarcinoma / Q000000981:diagnostic imaging / Q000276:immunology / Q000473:pathology*; D000818:Animals; D003110:Colonic Neoplasms / Q000000981:diagnostic imaging / Q000276:immunology / Q000473:pathology*; D008113:Liver Neoplasms / Q000000981:diagnostic imaging / Q000276:immunology / Q000473:pathology*; D008262:Macrophage Activation; D012157:Mononuclear Phagocyte System / Q000276:immunology*; D010587:Phagocytosis; D011877:Radionuclide Imaging; D051381:Rats; D011920:Rats, Inbred WF; D013668:Technetium Tc 99m Aggregated Albumin
D013668:Technetium Tc 99m Aggregated Albumin