[Specificity of fragmentation of DNA from pBR322 plasmid by Ca,Mg-dependent endonuclease from cell nuclei of human lymphocytes].
Fragmentation of the plasmid pBR322 DNA by a purified preparation of Ca/Mg-dependent endonuclease has been studied. It was shown that on the first steps of reaction the double-stranded cuts are introduced into the superhelical DNA independent of singlestranded ones. The doublestranded cuts are introduced into superhelical and linear DNA in 12 sites enriched with GC-pairs, 9 of them include pentanucleotide CGCGG(CCGCC) that is functionally significant. Relaxation of the plasmid DNA by topoisomerase I blocks the sitespecific action of the enzyme. Ca/Mg-dependent endonuclease is concluded to be topologically dependent enzyme, possibly, participating in the recombination processes.
Khodarev
N N
NN
Morozov
A Iu
AIu
Sokolova
I A
IA
Aleksandrova
S S
SS
Votrin
I I
II
D001483:Base Sequence; D002467:Cell Nucleus / Q000201:enzymology; D004247:DNA / Q000235:genetics / Q000378:metabolism*; D004260:DNA Repair; D004706:Endodeoxyribonucleases / Q000378:metabolism*; D006801:Humans; D006868:Hydrolysis; D007700:Kinetics; D008214:Lymphocytes / Q000201:enzymology*; D008969:Molecular Sequence Data; D010957:Plasmids
D004247:DNA; D004706:Endodeoxyribonucleases; C029367:calcium magnesium dependent endodeoxyribonuclease
Spetsifichnost' fragmentatsii DNK plazmidy pBR322 Ca/Mg-zavisimoĭ éndonukleazoĭ kletochnykh iader limfotsitov cheloveka.