[Chelating and antiradical effect of rutin during peroxidation of lipids from microsomes and liposomes].
The antioxidative effect of rutin (vitamin P) on Fe2+-induced lipid peroxidation (LPO) in bovine heart microsomes and lecithin liposomes was studied. It was shown that the LPO-induced inhibition of microsomes and liposomes in the presence of rutin occurs via two mechanisms, i.e., association of Fe2+ ions to form an inactive complex and a direct interaction between rutin and free radicals. The contribution of these mechanisms depends on the composition of the reaction mixture. In bovine heart microsomes and liposomes, ascorbic acid has a dual activity towards LPO. At high concentrations of Fe2+ necessary for LPO induction (approximately 1 x 10(-3) M), ascorbic acid blocks LPO, whereas at low Fe2+ concentrations (less than 1 x 10(-4) M) it has a prooxidative effect. A combined use of ascorbic acid and rutin results in an additive antioxidative effect at high Fe2+ concentrations (approximately 1.10(-3) M). However, at low Fe2+ concentrations rutin acts as an antagonist of the prooxidative effect of ascorbic acid.
Dorozhko
A I
AI
Brodskiĭ
A V
AV
Afanas'ev
I B
IB
D000818:Animals; D000975:Antioxidants; D001205:Ascorbic Acid / Q000494:pharmacology; D002417:Cattle; D005290:Ferric Compounds / Q000494:pharmacology; D066298:In Vitro Techniques; D007502:Iron Chelating Agents; D015227:Lipid Peroxidation / Q000187:drug effects*; D008081:Liposomes / Q000378:metabolism*; D008315:Malondialdehyde / Q000378:metabolism; D008861:Microsomes / Q000378:metabolism*; D012431:Rutin / Q000494:pharmacology*
D000975:Antioxidants; D005290:Ferric Compounds; D007502:Iron Chelating Agents; D008081:Liposomes; D008315:Malondialdehyde; D012431:Rutin; D001205:Ascorbic Acid
Khelatiruiushchee i antiradikal'noe deĭstvie rutina v protsesse perekisnogo okisleniia lipidov mikrosom i liposom.