Calculation of antibody affinity in homogeneous and heterogeneous systems.
Antibody affinity is an important determinant of all antibody-antigen reactions. A new computer program, AFCRV, was developed to calculate binding constants with data from a radioimmunoassay on most microcomputers in the laboratory by using constant-ratio dilution curves. Evaluation of a homogeneous or heterogeneous antibody in the presence of a single antigen can be accomplished.
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School of Agribusiness and Environmental Resources, Arizona State University, Tempe 85287-3306.
D000906:Antibodies / Q000276:immunology*; D000936:Antigen-Antibody Complex; D008962:Models, Theoretical; D011485:Protein Binding; D012984:Software
D000906:Antibodies; D000936:Antigen-Antibody Complex