title: Copper intake affects rat heart performance during ischemia-reperfusion: possible relation to altered lipid and fatty acid metabolism.
abstract: Hearts from rats fed low copper (1.3 mg copper/kg diet) or a copper-supplemented diet (243 mg copper/kg diet) were perfused for 90 min according to the Langendorff method. The perfusion protocol included 30 min normoxia, 30 min ischemia and 30 min reperfusion. After 90 min perfusion, hearts from the low copper group had gained more weight, had lower coronary perfusion pressure, developed less force of contraction and secreted less 6-keto PGF1 alpha into the perfusate than hearts from the copper-supplemented group. After perfusion, the major lipid change in the hearts from both groups was a 85-90% decrease in total triacylglycerol. In both groups, stearic acid and arachidonic acid (mg%) were increased in the triacylglycerol fraction after heart perfusion. The quantitative (mg/g) decrease in the triacylglycerol content of stearic acid and arachidonic acid was significantly less in the copper-supplemented group. After perfusion, dihomo-gamma-linolenic acid (mg/g) was lower in heart phospholipids from the low copper group. Dihomo-gamma-linolenic/arachidonic acid (microgram/mg) was significantly decreased after perfusion only in the hearts from the low copper group. Lipid and fatty acid changes in the hearts of the rats fed low dietary copper may contribute to abnormal heart function in this group.
authors.affiliation: Efamol Research Institute, Kentville, Nova Scotia, Canada.
authors.forename: S C
authors.initials: SC
authors.lastname: Cunnane
authors.forename: K R
authors.initials: KR
authors.lastname: McAdoo
authors.forename: M
authors.initials: M
authors.lastname: Karmazyn
mesh_terms: D000818:Animals; D003300:Copper / Q000172:deficiency*; D005227:Fatty Acids / Q000378:metabolism; D006321:Heart / Q000502:physiology*; D050356:Lipid Metabolism; D008297:Male; D011453:Prostaglandins / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D015427:Reperfusion Injury / Q000503:physiopathology*
chemical_list: D005227:Fatty Acids; D011453:Prostaglandins; D003300:Copper