[Calcium transport in proteoliposomes incorporating membranes of skeletal muscle T-system].
Using the radioisotope method, the Ca2+ transport through proteoliposomes was investigated. The proteoliposomes originating from total brain lipids and skeletal muscle T-system membranes of the rabbit were shown to possess a Ca2+ permeability which can be stimulated by 1.4-dihydroxypyridine derivatives (10(-9)-10(-7) M). Verapamil and Cd2+ (10(-5) M and 10(-3) M, respectively) inhibit the Ca2+ permeability of proteoliposomes stimulated by dihydroxypyridine derivatives. The activating effect of the latter depends on the microviscosity of the proteoliposome lipid bilayer. An addition of cholesterol to brain phospholipids at a ratio of 1:5 increases the stimulating effect of dihydroxypyridine by 50%.
Rozhmanova
O M
OM
Dubur
G Ia
GIa
Kastron
V V
VV
Stel'makh
L N
LN
Voronina
S G
SG
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D001923:Brain Chemistry; D002104:Cadmium / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D004095:Dihydropyridines / Q000494:pharmacology; D066298:In Vitro Techniques; D008566:Membranes / Q000378:metabolism; D009132:Muscles / Q000378:metabolism*; D010539:Permeability; D011510:Proteolipids / Q000378:metabolism*; D011817:Rabbits
D002121:Calcium Channel Blockers; D004095:Dihydropyridines; D011510:Proteolipids; C020485:proteoliposomes; D002104:Cadmium; D002118:Calcium
Izuchenie kal'tsievogo transporta v proteoliposomakh, vkliuchaiushchikh membrany T-sistemy skeletnykh myshts.