Distribution of vasoactive intestinal peptide-sensitive adenylate cyclase activity along the nephron.
The effect of vasoactive intestinal peptide (VIP) on adenylate cyclase activity in individual segments of the rabbit nephron was examined. VIP caused a significant increase in adenylate cyclase activity in the distal convoluted tubule and in the cortical and medullary portions of the collecting tubule. VIP also caused a concentration-dependent increase in adenylate cyclase activity in rat and dog collecting tubules. The results demonstrate that VIP has a direct stimulatory effect on tubular adenylate cyclase which may be involved in the increase in electrolyte excretion observed with VIP.
Edwards
R M
RM
Department of Pharmacology, Smith Kline and French Laboratories, Swedeland, PA 19406-0939.
D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D004285:Dogs; D007685:Kidney Tubules, Collecting / Q000187:drug effects / Q000201:enzymology; D009399:Nephrons / Q000187:drug effects / Q000201:enzymology*; D011817:Rabbits; D014660:Vasoactive Intestinal Peptide / Q000494:pharmacology*
D014660:Vasoactive Intestinal Peptide; D000262:Adenylyl Cyclases