Accumulation of glycation products in alpha-H pig lens crystallin and its bearing to diabetic cataract genesis.
The incorporation of 14C-glucose in native pig crystallin by in vitro incubation was found, after subsequent dialysis, to affect all 5 classes of crystallin separated by Sepharose CL-6B column chromatography. Though the radioactivity of the alpha-H fraction was three times greater than that of any of the others, autoradiographs of SDS-PAGE gels showed 14C-glucose adducts to be present in all soluble protein subunits, without there being any evidence of preferential glycation of the alpha-H subunits. The concentration of stable glycation products in the alpha-H chromatographic fraction of soluble crystallins is suggested to be due the addition of glycated material to this fraction as result of glycation-induced hyperaggregation, and not because the alpha-H subunits were especially susceptible to glycation.
Vidal
P
P
Service of Endocrinology and Nutrition, Hospital General de Galicia, University of Santiago de Compostela, Spain.
Cabezas-Cerrato
J
J
D000818:Animals; D001345:Autoradiography; D002386:Cataract / Q000209:etiology*; D002850:Chromatography, Gel; D048909:Diabetes Complications; D004591:Electrophoresis, Polyacrylamide Gel; D005947:Glucose / Q000378:metabolism*; D006801:Humans; D007908:Lens, Crystalline / Q000378:metabolism*; D013552:Swine
D005947:Glucose