Antibody response to trimellityl hemoglobin in trimellitic anhydride-induced lung injury.
Sprague-Dawley rats were exposed to trimellitic anhydride by inhalation, and the antibody response to trimellityl (TM)-conjugated hemoglobin (HB) and TM rat serum albumin (RSA) was compared. Groups of rats were exposed to trimellitic anhydride by inhalation 6 hours per day for 2, 6, or 10 days at 100 micrograms/m3 and compared to a control group exposed to filtered air. The IgG antibody response to TM-HB in both serum and bronchoalveolar lavage (BAL) fluid was measured with ELISA. IgG antibody levels to TM-HB rose significantly throughout the exposure. A positive correlation was found between IgG to TM-HB in serum and BAL fluid. In addition, this response in both serum and BAL fluid correlated with the IgG antibody response to TM-RSA. Cross-inhibition studies indicated the existence of shared antigenic determinants on TM-RSA and TM-HB. The IgG antibody to both antigens was specific for new antigenic determinants and not for the TM hapten.
Pien
L C
LC
Department of Medicine, Northwestern University Medical School, Chicago, Ill.
Zeiss
C R
CR
Leach
C L
CL
Hatoum
N S
NS
Levitz
D
D
Garvin
P J
PJ
Patterson
R
R
D000818:Animals; D001667:Binding, Competitive; D001992:Bronchoalveolar Lavage Fluid / Q000032:analysis / Q000473:pathology; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D006454:Hemoglobins / Q000276:immunology*; D007074:Immunoglobulin G / Q000096:biosynthesis*; D008297:Male; D010795:Phthalic Acids / Q000276:immunology*; D010796:Phthalic Anhydrides / Q000276:immunology*; D051381:Rats; D011919:Rats, Inbred Strains; D012130:Respiratory Hypersensitivity / Q000097:blood / Q000139:chemically induced* / Q000276:immunology; D012709:Serum Albumin / Q000276:immunology
D006454:Hemoglobins; D007074:Immunoglobulin G; D010795:Phthalic Acids; D010796:Phthalic Anhydrides; D012709:Serum Albumin; C015559:trimellitic anhydride