Conserved region of the rat alpha-lactalbumin promoter is a target site for protein binding in vitro.
Nuclear extracts from mammary glands of lactating rats contain DNA binding protein(s) with high affinity to the region -125 to -85 of the rat alpha-lactalbumin promoter. The protected sequence is part of a mammary conserved sequence described by Hall et al. [(1987) Biochem. J. 242, 735-742]. Comparison of the DNA protein-binding sites in the 5' flanking sequence of the rat alpha-lactalbumin and mouse whey acidic protein genes suggests that the consensus sequence 5'-TGGCARNNWCKKC-3' is the protein(s) recognition site. This binding site is part of a highly conserved region [consensus sequence, TGGCAGSCTCGGCST(G)YTCTCTCT(NTG)TGGCARA] present in the promoter of four whey protein genes.
Lubon
H
H
Laboratory of Biochemistry and Metabolism, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, MD 20892.
Hennighausen
L
L
D000818:Animals; D001483:Base Sequence; D001667:Binding, Competitive; D004268:DNA-Binding Proteins / Q000378:metabolism; D005260:Female; D007768:Lactalbumin / Q000235:genetics*; D008321:Mammary Glands, Animal / Q000378:metabolism; D051379:Mice; D008894:Milk Proteins / Q000235:genetics; D009687:Nuclear Proteins / Q000378:metabolism; D011247:Pregnancy; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D051381:Rats; D000067816:Whey Proteins
D004268:DNA-Binding Proteins; D008894:Milk Proteins; D009687:Nuclear Proteins; D000067816:Whey Proteins; D007768:Lactalbumin