Further characterization of porcine kidney aminoacylase I reveals close similarity to 'renal dipeptidase'.
We present data indicating that aminoacylase I (EC 3.5.1.14) from porcine kidney and 'renal dipeptidase' (EC 3.4.13.11) are closely related. We show that, in situ, a considerable fraction of aminoacylase activity ist attached to membranes. Incubation of washed microsomal membranes with phospholipase C from B. cereus results in the rapid solubilization of aminoacylase I, suggesting that aminoacylase--as shown for renal dipeptidase before--bears a glycolipid 'membrane anchor'. In agreement with this assumption, purified aminoacylase was found to contain myo-inositol, a characteristic component of phosphatidylinositol-anchored membrane proteins. A reexamination of the molecular mass of purified aminoacylase yielded values (46,000 +/- 2,000 Da by SDS polyacrylamide electrophoresis, 98,000 +/- 5,000 Da by sedimentation equilibrium centrifugation) similar to those reported for renal dipeptidase. The enzymes coelute during most of the procedures applied in the purification of aminoacylase or renal dipeptidase, but can be separated by hydrophobic interaction chromatography. A survey of the literature revealed a series of additional features of aminoacylase I and renal dipeptidase (amino-acid composition, isoelectric points, metal dependence, and more) that are strikingly similar.
Heese
D
D
Institut für Physiologische Chemie, Universität Marburg.
Löffler
H G
HG
Röhm
K H
KH
D000581:Amidohydrolases / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000906:Antibodies; D002241:Carbohydrates / Q000032:analysis; D004150:Dipeptidases / Q000276:immunology / Q000378:metabolism*; D007668:Kidney / Q000201:enzymology*; D007672:Kidney Cortex / Q000201:enzymology; D007700:Kinetics; D008861:Microsomes / Q000201:enzymology; D008970:Molecular Weight; D013552:Swine
D000596:Amino Acids; D000906:Antibodies; D002241:Carbohydrates; D004150:Dipeptidases; C038937:dipeptidase; D000581:Amidohydrolases; C019672:aminoacylase I