Effect of elastase on blood pressure and urinary excretions of prostaglandins and catecholamines in the spontaneously hypertensive rat (SHR).
The effects of elastase on blood pressure (B.P.) and the urinary excretions of prostaglandins (PGs) and catecholamines in the spontaneously hypertensive rat (SHR) were investigated. Male SHRs (n = 20), castrated at 4 weeks of age, were divided into 2 groups. Elastase was then dissolved in saline (5 mg/ml) and injected subcutaneously (10 mg/kg of body weight) into the SHRs of one of the 2 groups (n = 10) at 5 to 13 weeks of age, and the same dose of saline only was injected subcutaneously into the SHRs of the other group (n = 10) as a control during the same period. B.P. was measured by the tail-cuff method every 2 weeks. Urine sampling and measurement of body weight (B.Wt.) were also performed every 2 weeks. The B.P. in the elastase-administered group (184.1 +/- 3.3 mmHg) was found to be significantly lower (p less than 0.02) than that of the control group (198.0. +/- 4.3) at 9 weeks of age. A significant difference (p less than 0.01) in B.P. between the control group (204.0 +/- 4.5) and the elastase-administered group (187.3 +/- 2.7) was also observed at 13 weeks of age. The urinary excretion of 6-keto-prostaglandin F1 alpha (6-keto-PGF1 alpha) in the control group (5.1 +/- 0.4 ng/day/100 g B.Wt.) at 11 weeks of age was significantly lower (p less than 0.05) than that of the elastase-administered group (10.4 +/- 1.3).(ABSTRACT TRUNCATED AT 250 WORDS)
Watanabe
M
M
Second Department of Internal Medicine, Nihon University School of Medicine, Tokyo, Japan.
Honda
M
M
Hatano
M
M
D000818:Animals; D001794:Blood Pressure; D001835:Body Weight; D002395:Catecholamines / Q000652:urine*; D003404:Creatinine / Q000097:blood; D006973:Hypertension / Q000652:urine*; D008297:Male; D009318:Natriuresis; D010196:Pancreatic Elastase / Q000378:metabolism*; D011453:Prostaglandins / Q000652:urine*; D051381:Rats; D011918:Rats, Inbred SHR
D002395:Catecholamines; D011453:Prostaglandins; D003404:Creatinine; D010196:Pancreatic Elastase