Quantitative immunohistochemical analysis of VIP-neurons in the rat visual cortex.
A critical appraisal of quantitative immunohistochemistry of neuropeptides is presented defining the main criteria of selecting the type of immune-staining and preparation suitable for these investigations. As an example of meeting the established criteria, the immunohistochemical demonstration of vasoactive intestinal polypeptide (VIP)-containing neurons in the rat brain and the processing of VIP-immunostained preparations for computer-controlled image analysis are described.
Hajós
F
F
Department of Anatomy, University of Veterinary Sciences, Budapest, Hungary.
Zilles
K
K
D000818:Animals; D005260:Female; D007150:Immunohistochemistry; D008297:Male; D009474:Neurons / Q000032:analysis* / Q000648:ultrastructure; D051381:Rats; D014660:Vasoactive Intestinal Peptide / Q000032:analysis*; D014793:Visual Cortex / Q000032:analysis* / Q000648:ultrastructure
D014660:Vasoactive Intestinal Peptide