The effects of ultrarapid freezing on meiotic and mitotic spindles of mouse oocytes and embryos.
Preovulatory mouse oocytes and 2-cell embryos were frozen with dimethyl sulfoxide and propanediol by an ultrarapid method. The survival of frozen oocytes was low (33-34%) compared to that of 2-cell embryos (78-79%) with either cryoprotectant. Development to blastocysts after postthaw culture was about 7-15% for oocytes and 79-80% for the embryos. Ultrarapid freezing preserves cell structure quite well as revealed by electron microscopy, but meiotic oocytes and late 2-cell embryos undergoing mitosis showed evidence of spindle disorganization involving loss or clumping of microtubules resulting in some scattering of chromosomes. Embryos developed from frozen eggs showed clear evidence of micronuclear formation and incomplete incorporation of chromosomal material into main nuclei. These experiments confirm our observations on freezing of human oocytes and show that spindle microtubules are sensitive to freeze-thawing and that cryopreservation could cause chromosomal aberrations during early development. A cautious approach to the introduction of oocyte freezing in human in vitro fertilization (IVF) programs is advocated.
Sathananthan
A H
AH
Department of Obstetrics and Gynaecology, National University Hospital, Singapore.
Ng
S C
SC
Trounson
A O
AO
Bongso
A
A
Ratnam
S S
SS
Ho
J
J
Mok
H
H
Lee
M N
MN
D000818:Animals; D004121:Dimethyl Sulfoxide; D004622:Embryo, Mammalian / Q000648:ultrastructure*; D005260:Female; D005615:Freezing; D066298:In Vitro Techniques; D008540:Meiosis; D051379:Mice; D008854:Microscopy, Electron; D008870:Microtubules / Q000648:ultrastructure; D009865:Oocytes / Q000648:ultrastructure*; D011309:Preservation, Biological / Q000379:methods*; D019946:Propylene Glycol; D011409:Propylene Glycols; D008941:Spindle Apparatus / Q000648:ultrastructure*
D011409:Propylene Glycols; D019946:Propylene Glycol; D004121:Dimethyl Sulfoxide