title: Estrogen effects on histone messenger ribonucleic acid levels in the rat uterus.
abstract: RNA was isolated from uteri of immature rats before and after estrogen treatment. The concentration of histone mRNA was analyzed by Northern hybridization and compared with messenger RNA concentration of alpha-actin, beta-actin, and beta-tubulin. Steady state levels of common histone mRNAs did not change up to 9 h after hormone administration. After that time the histone mRNA levels increased significantly and reached a maximum at 18 h, several hours later than the time of maximal histone protein biosynthesis induced by estrogen. The concentration of control mRNAs (alpha- and beta-actin and beta-tubulins) increased shortly after estradiol injection and reached a peak at 9 h. These results show that the pattern of histone gene expression induced by estrogen has some features similar to those observed during embryogenesis.
authors.affiliation: Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin-Madison 53706.
authors.forename: J Z
authors.initials: JZ
authors.lastname: Guo
authors.forename: J
authors.initials: J
authors.lastname: Gorski
mesh_terms: D000818:Animals; D004261:DNA Replication / Q000187:drug effects; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D006657:Histones / Q000235:genetics*; D007700:Kinetics; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D051381:Rats; D012016:Reference Values; D014599:Uterus / Q000187:drug effects / Q000378:metabolism*
chemical_list: D006657:Histones; D012333:RNA, Messenger; D004958:Estradiol