The effect of splanchnic nerve stimulation and neuropeptide Y on cholera secretion and release of vasoactive intestinal polypeptide in the feline small intestine.
The effect of sympathetic nerve stimulation and intra-arterial infusion of neuropeptide Y (NPY) on net fluid secretion and release of vasoactive intestinal polypeptide (VIP) was studied in the cat small intestine during a secretion due to cholera toxin. Activation of the splanchnic nerves (4 Hz, 5 ms, 5 V) decreased net fluid secretion to 57 +/- 10% of control. Concomitantly, the release of VIP was reduced to less than 50%. Furthermore, close i.a. infusion of NPY (estimated increase in plasma concentration 75 nmol l-1) reduced the net fluid secretion and VIP release to 27 +/- 5 and 28 +/- 4% of the pre-stimulatory value. The correlation between the decrease in net fluid secretion and reduction in VIP release showed a strong positive correlation (r = 0.83). These results strongly indicate that the antisecretory effect of sympathetic nerve stimulation during cholera diarrhoea is mediated by inhibition of secretory VIP neurons in the intestinal mucosa. A similar mechanism is also proposed for the intravascularly administered NPY.
Sjöqvist
A
A
Department of Physiology, University of Götenborg, Sweden.
Fahrenkrug
J
J
Jodal
M
M
Lundgren
O
O
D000818:Animals; D002415:Cats; D002772:Cholera Toxin / Q000008:administration & dosage*; D007419:Intestinal Secretions / Q000187:drug effects; D007421:Intestine, Small / Q000187:drug effects / Q000378:metabolism*; D009478:Neuropeptide Y / Q000502:physiology*; D013153:Splanchnic Nerves / Q000502:physiology*; D014660:Vasoactive Intestinal Peptide / Q000378:metabolism*
D009478:Neuropeptide Y; D014660:Vasoactive Intestinal Peptide; D002772:Cholera Toxin