Biochemical changes in alpha-naphthyl isothiocyanate-induced chronic cholangitis in the rat.
Male Wistar rats were treated for 3 weeks with alpha-naphthyl isothiocyanate (ANIT, 5.4 mmol per kg food). Chronic necrotic cholangitis without pronounced transaminasemia and hyperbilirubinemia developed. The activity of 5'-nucleotidase in liver plasma membrane preparations was strongly depressed (3.5 times) while the activities of K+-, Na+- and Mg++-ATPases were not affected. The liver microsomal cytochrome P-450 and cytochrome b5 contents decreased. Elevation of reduced glutathione liver content after challenge with ANIT was recorded. The observed biochemical changes may be important for the pathogenesis of ANIT-induced chronic cholangitis.
Nikolaev
V
V
Institute of Gastroenterology and Nutrition, Medical Academy, Sofia, Bulgaria.
Kerimova
M
M
Naydenova
E
E
Ivanov
E
E
Dontchev
N
N
Adjarov
D
D
D015058:1-Naphthylisothiocyanate; D000818:Animals; D002761:Cholangitis / Q000139:chemically induced / Q000378:metabolism*; D002908:Chronic Disease; D003580:Cytochromes / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values
D003580:Cytochromes; D015058:1-Naphthylisothiocyanate; D005978:Glutathione