title: Variability in the amino terminus of myosin light chain 1.
abstract: Three naturally occurring variants of myosin light chain 1, type I, II, and III from avian fast-twitch muscle, have been analyzed by reverse-phase HPLC peptide mapping and amino acid sequencing. Difference peptides were absent from accompanying digests of the related protein, myosin light chain 3, indicating that the heterogeneity was located in the N-terminal 50 residues unique to light chain 1. The type II variant possessed the previous published sequence for the protein [Nabeshima Y., Fujii-Kuriyama, Y., Muramatsu, M., & Ogata, K. (1984) Nature (London) 308, 333-338]. The type I variant, which migrates faster than the type II on SDS gene electrophoresis, contained a Pro----Ala substitution at residue 15, turning the Lys-Pro-(Ala)5(Pro-Ala)7 stretch in this region into Lys-Pro-(Ala)7(Pro-Ala)6. The type III variant, which migrates just faster than the type I, had an (Ala)2 deletion in the (Ala)5 run, yielding Lys-Pro-(Ala)3-(Pro-Ala)7. As indicated by the SDS gel migration rates, the type I and III variants are significantly shorter in length than the type II. The benign nature of the changes is consistent with a flexible arm function for the N-terminal region of light chain 1, with the structural changes in the variants occurring in the spacer region of the arm.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: State University of New York--Health Science Center, Brooklyn 11203.
authors.forename: J I
authors.initials: JI
authors.lastname: Rushbrook
authors.forename: A G
authors.initials: AG
authors.lastname: Wadewitz
authors.forename: M
authors.initials: M
authors.lastname: Elzinga
authors.forename: T T
authors.initials: TT
authors.lastname: Yao
authors.forename: R G
authors.initials: RG
authors.lastname: Somes
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002645:Chickens; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D014644:Genetic Variation; D008969:Molecular Sequence Data; D009218:Myosins / Q000235:genetics*; D010446:Peptide Fragments; D012697:Serine Endopeptidases; D014357:Trypsin
chemical_list: D010446:Peptide Fragments; D012697:Serine Endopeptidases; C028344:glutamyl endopeptidase; D014357:Trypsin; D009218:Myosins