Antigens of Timothy and other grass pollen extracts identified by monoclonal antibodies.
Using a series of monoclonal antibodies raised against pollen extract of timothy grass pollen (Phleum pratense) we identified individual (species-specific) and cross-reactive epitopes shared by different grass and tree pollens in EIA experiments. These epitopes are heterogenously distributed as detected by means of immunoblotting. Crossed radioimmunoelectrophoresis experiments revealed binding of monoclonal antibodies to major allergens of timothy grass pollen extract.
Müller
W D
WD
Institute of Clinical Immunology, Friedrich Schiller University, Jena, German Democratic Republic.
Diener
C
C
Jung
K
K
Jäger
L
L
D000911:Antibodies, Monoclonal / Q000276:immunology; D000937:Antigen-Antibody Reactions; D000941:Antigens / Q000276:immunology / Q000302:isolation & purification*; D003429:Cross Reactions; D010936:Plant Extracts / Q000276:immunology; D006109:Poaceae / Q000276:immunology*; D011058:Pollen / Q000276:immunology*; D013045:Species Specificity; D014197:Trees
D000911:Antibodies, Monoclonal; D000941:Antigens; D010936:Plant Extracts