Cytoskeletal architecture and immunocytochemical localization of fodrin in the terminal web of the ciliated epithelial cell.
In order to understand the cytoskeletal architecture at the terminal web of the ciliated cell, we examined chicken tracheal epithelium by quick-freeze deep-etch (QFDE) electron microscopy combined with immunocytochemistry of fodrin. At the terminal web, the cilia ended into the basal bodies and then to the rootlets. The rootlets were composed of several filaments and globular structures attached regularly to them. Decoration with myosin subfragment 1 (S1) revealed that some actin filaments ran parallel to the apical plasma membrane between the basal bodies, and other population traveled perpendicularly or obliquely, i.e., along the rootlets. Some actin filaments were connected to the surface of the basal bodies and the basal feet. Among the basal bodies and the rootlets there existed three kinds of fine crossbridges, which were not decorated with S1. In the deeper part of the terminal web, intermediate filaments were observed between the rootlets and were sometimes crosslinked with the rootlets. Immunocytochemistry combined with the QFDE method revealed that fodrin was a component of fine crossbridges associated with the basal bodies. We concluded that an extensive crosslinker system among the basal bodies and the rootlets along with networks of actin and intermediate filaments formed a structural basis for the effective beating of cilia.
Kobayashi
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Department of Anatomy and Cell Biology, School of Medicine, University of Tokyo, Japan.
Hirokawa
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D000199:Actins / Q000032:analysis; D000818:Animals; D002352:Carrier Proteins / Q000032:analysis*; D002645:Chickens; D002923:Cilia / Q000032:analysis; D003599:Cytoskeleton / Q000648:ultrastructure*; D004847:Epithelial Cells; D004848:Epithelium / Q000032:analysis; D005260:Female; D015151:Immunoblotting; D007150:Immunohistochemistry; D008565:Membrane Proteins / Q000032:analysis*; D008840:Microfilament Proteins / Q000032:analysis*; D008854:Microscopy, Electron; D014132:Trachea / Q000166:cytology; D013049:Spectrin
D000199:Actins; D002352:Carrier Proteins; D008565:Membrane Proteins; D008840:Microfilament Proteins; D013049:Spectrin