title: Characterization of muscarinic receptors in human pancreatic membranes.
abstract: Crude membranes (27,000 x g pellets) from three normal human pancreata were prepared. Muscarinic receptors were investigated by the ability of three antagonists (atropine, pirenzepine, and AF-DX 116) and three agonists (carbamylcholine, oxotremorine, and pilocarpine) to inhibit [3H]NMS binding. These receptors showed for pirenzepine and AF-DX 116 a M2 beta specificity, typical of secretory glands and smooth muscle, that was comparable to that of rat pancreatic membranes, i.e., a low affinity for the two antagonists (Ki of 0.4 and 0.2 microM, respectively). In addition, these receptors were predominantly in a low affinity state for the agonist carbamylcholine (Ki of 100 microM).
authors.affiliation: Department of Biochemistry and Nutrition, Medical School, Université Libre de Bruxelles, Belgium.
authors.forename: M
authors.initials: M
authors.lastname: Waelbroeck
authors.forename: J
authors.initials: J
authors.lastname: Camus
authors.forename: M
authors.initials: M
authors.lastname: Tastenoy
authors.forename: P
authors.initials: P
authors.lastname: De Neef
authors.forename: J L
authors.initials: JL
authors.lastname: Scemama
authors.forename: D
authors.initials: D
authors.lastname: Fourmy
authors.forename: N
authors.initials: N
authors.lastname: Vaysse
authors.forename: L
authors.initials: L
authors.lastname: Pradayrol
authors.forename: P
authors.initials: P
authors.lastname: Robberecht
authors.forename: J
authors.initials: J
authors.lastname: Christophe
mesh_terms: D002217:Carbachol / Q000378:metabolism; D002462:Cell Membrane / Q000032:analysis / Q000648:ultrastructure; D006801:Humans; D010095:Oxotremorine / Q000378:metabolism; D010179:Pancreas / Q000032:analysis* / Q000648:ultrastructure; D010862:Pilocarpine / Q000378:metabolism; D010890:Pirenzepine / Q000031:analogs & derivatives / Q000378:metabolism; D011976:Receptors, Muscarinic / Q000032:analysis* / Q000378:metabolism
chemical_list: D011976:Receptors, Muscarinic; D010862:Pilocarpine; D010890:Pirenzepine; D010095:Oxotremorine; D002217:Carbachol; C048593:otenzepad