Legionella pneumophila: initial detection through evaluation of Giemsa-stained smears of a bronchial aspirate.
The presence of Legionella species in a bronchial aspirate was strongly suspected by the visualization on Giemsa-stained smears of slender violaceous intra- and extracellular bacilli despite the absence of fluorescence on smears stained with L. pneumophila serogroup 1 conjugate. Subsequently, cultures of the aspirate grew L. pneumophila reactive with serogroup 6 fluorescein-conjugated antibody.
Reitano
M
M
Department of Microbiology, Mount Sinai Hospital, New York, N.Y. 10029-6574.
Engler
H D
HD
Bottone
E J
EJ
D003581:Cytodiagnosis; D006801:Humans; D007875:Legionella / Q000302:isolation & purification*; D007876:Legionellosis / Q000175:diagnosis / Q000473:pathology; D008168:Lung / Q000382:microbiology* / Q000473:pathology