1,25-Dihydroxyvitamin D3 stimulates avian and mammalian cartilage growth in vitro.
We addressed the question of whether 1,25-dihydroxyvitamin D3 (1,25-(OH)2D) could directly stimulate cartilage growth in vitro. Pelvic leaflets from chick embryos and scapular growth plates from fetal pigs were organ cultured in serum-free medium in the presence and absence of 1,25-(OH)2D. After 3 days of incubation, 1,25-(OH)2D had increased the pelvic cartilage wet weight 42% and the dry weight 32% above the weight of cartilages incubated in medium alone. 1,25-(OH)2D (10(-9) M-10(-12) M) caused a dose-dependent increase in weight, with maximal increases at 10(-9) M. Furthermore, two deuterized derivatives of 1,25-(OH)2D, 26,27-D6-1,25-(OH)2D3 and 24,26,27-D8-1,25-(OH)2D3, stimulated pelvic cartilage growth in vitro. 26,27-D6-1,25-(OH)2D stimulated increases in growth plate weight above growth plates incubated in medium alone. 26,27-D6-1,25-(OH)2D3 appeared to be potent at lower concentrations than 1,25-(OH)2D on growth plate cartilage. Thus, 1,25-(OH)2D stimulated in vitro growth in two growing cartilage models, the avian pelvic cartilage and the mammalian scapular growth plate cartilage.
Burch
W M
WM
Department of Medicine, Duke University Medical Center, Durham, NC.
Lopez-Claros
M
M
Uskokovic
M R
MR
Drezner
M K
MK
D000818:Animals; D002117:Calcitriol / Q000494:pharmacology*; D002356:Cartilage / Q000166:cytology* / Q000187:drug effects / Q000196:embryology; D002455:Cell Division / Q000187:drug effects; D002642:Chick Embryo; D005333:Fetus; D006132:Growth Plate / Q000166:cytology / Q000187:drug effects / Q000196:embryology; D009924:Organ Culture Techniques; D012016:Reference Values; D013552:Swine
D002117:Calcitriol