Rapid separation of flavonoids by analytical high-speed counter-current chromatography.
A commercial model of the analytical high-speed counter-current chromatography instrument was used for separation of flavonoids from a crude ethanol extract of dried fruits of sea buckthorn (Hippophae rhamnoides). Using a two-phase solvent system of chloroform-methanol-water (4:3:2), a five-fold increase in flow-rate of the mobile phase from 60 to 300 ml/h resulted in a rapid separation of five components in less than 15 min without significant loss in peak resolution. Major flavonoid component, isorhamnetin, was identified in its pure state by mass spectrometric analysis.
Zhang
T Y
TY
Laboratory of Technical Development, National Heart, Lung, and Blood Institute, Bethesda, MD 20892.
Xiao
R
R
Xiao
Z Y
ZY
Pannell
L K
LK
Ito
Y
Y
D003377:Countercurrent Distribution / Q000295:instrumentation; D005419:Flavonoids / Q000302:isolation & purification*; D013058:Mass Spectrometry; D010944:Plants / Q000032:analysis*
D005419:Flavonoids