Separation of hemagglutinin and neuraminidase from influenza virus membrane by column displacement electrophoresis (isotachophoresis) with preservation of their activities.
Triton X-100-solubilized membrane glycoproteins (neuraminidase and hemagglutinin) from purified equine influenza virus particles were separated by column displacement electrophoresis (isotachophoresis) in the presence of Pharmalyte spacers. Electrophoresis was performed in a 1.80 cm glass electrophoresis column with Sephadex G-25 Fine serving as supporting medium. Triton X-100 was present in the system to suppress protein aggregation. Neuraminidase and hemagglutinin activities were preserved and appeared in the electropherogram as separate peaks with some overlapping.
Johansson
G
G
Institute of Biochemistry, University of Uppsala, Sweden.
Abusugra
I
I
Lövgren
K
K
Morein
B
B
D002462:Cell Membrane / Q000032:analysis / Q000201:enzymology; D004586:Electrophoresis; D006389:Hemagglutinins, Viral / Q000302:isolation & purification*; D009980:Influenza A virus / Q000032:analysis*; D009439:Neuraminidase / Q000302:isolation & purification*
D006389:Hemagglutinins, Viral; D009439:Neuraminidase