Use of non-radioactive DNA probes for the characterization of adult T-cell leukemia cells.
DNA fragments were labeled with dinitrophenyl (DNP) residues by the reaction with 2,4-dinitrobenzaldehyde in alkaline condition and the labeled DNA was used as a probe for non-radioactive in situ hybridization. DNP-labeled DNA probes for T cell receptor beta chain, c-myc and HTLV-1 were hybridized in situ to mRNA on cell specimens fixed with Carnoy's fixative. DNA-mRNA hybrids were detected immunohistochemically using anti-DNP antibodies. Cytoplasms of adult T cell leukemia cells were stained with varied intensity when these probes were used. More than 70% of cells were positively stained with T cell receptor probe. However, less than 30% of cells were stained with c-myc and HTLV-1 probes. The present study indicates that non-radioactive in situ hybridization can be used for the characterization and classification of leukemia.
Moriuchi
T
T
Department of Cell Biology, Tokai University School of Medicine, Isehara, Japan.
Koji
T
T
Nakane
P K
PK
Yoshida
M
M
Moriuchi
J
J
Arimori
S
S
D000818:Animals; D004247:DNA / Q000032:analysis*; D015342:DNA Probes; D004279:DNA, Viral / Q000032:analysis; D005796:Genes; D015368:Human T-lymphotropic virus 1 / Q000235:genetics; D006801:Humans; D015458:Leukemia, T-Cell / Q000235:genetics*; D051379:Mice; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D011519:Proto-Oncogenes; D011948:Receptors, Antigen, T-Cell / Q000235:genetics
D015342:DNA Probes; D004279:DNA, Viral; D011948:Receptors, Antigen, T-Cell; D004247:DNA