High-performance liquid chromatographic determination of molluscicidal saponins from Phytolacca dodecandra (Phytolaccaceae).
A high-performance liquid chromatographic method is described for the determination of oleanane saponins in Phytolacca dodecandra (Phytolaccaceae), a plant with potent molluscicidal properties. The molluscicidal monodesmosidic saponins of the berries were determined at 254 nm as their 4-bromophenacyl derivatives, whereas the non-derivatized bidesmosidic saponins, lacking a free carboxyl group, were determined at 206 nm. A comparison of different extraction procedures showed that with cold water predominantly monodesmosidic saponins were obtained, whereas hot water gave mainly bidesmosidic (non-molluscicidal) saponins.
Slacanin
I
I
Institut de Pharmacognosie et Phytochimie, Ecole de Pharmacie de l'Université de Lausanne, Switzerland.
Marston
A
A
Hostettmann
K
K
D002851:Chromatography, High Pressure Liquid; D008975:Molluscacides / Q000302:isolation & purification* / Q000633:toxicity; D010936:Plant Extracts / Q000032:analysis; D010947:Plants, Toxic / Q000032:analysis*; D012503:Saponins / Q000302:isolation & purification* / Q000633:toxicity; D013056:Spectrophotometry, Ultraviolet
D008975:Molluscacides; D010936:Plant Extracts; D012503:Saponins