The plasma membrane and mitochondrial membrane potentials of Plasmodium yoelii.
1. The plasma membrane potential and the mitochondrial membrane potential of P. yoellii was examined by fluorescence microscopy using rhodamine 123 and by transmembrane distribution of tetraphenylphosphonium. 2. The mitochondrion of P. yoelii, free of gametocyte stage, maintained a high negative inside membrane potential. 3. Deprivation of glucose in incubation medium largely abolished the plasma membrane potential but not the mitochondrial membrane potential. 4. Studies with metabolic inhibitors showed that the mitochondrial membrane potential constituted a marginal portion as compared with the plasma membrane potential in intact infected erythrocytes.
Izumo
A
A
Department of Medical Zoology, Osaka City University Medical School, Japan.
Tanabe
K
K
Kato
M
M
D000818:Animals; D000963:Antimetabolites / Q000494:pharmacology; D002462:Cell Membrane / Q000378:metabolism; D005456:Fluorescent Dyes; D005947:Glucose / Q000494:pharmacology; D007425:Intracellular Membranes / Q000378:metabolism; D008564:Membrane Potentials / Q000187:drug effects; D008928:Mitochondria / Q000378:metabolism; D009861:Onium Compounds / Q000493:pharmacokinetics; D009943:Organophosphorus Compounds / Q000493:pharmacokinetics; D010967:Plasmodium yoelii / Q000187:drug effects / Q000378:metabolism*; D020112:Rhodamine 123; D012235:Rhodamines
D000963:Antimetabolites; D005456:Fluorescent Dyes; D009861:Onium Compounds; D009943:Organophosphorus Compounds; D012235:Rhodamines; D020112:Rhodamine 123; D005947:Glucose; C013289:tetraphenylphosphonium