Effect of aging on hepatic clearance of vasopressin in Fischer 344 rats.
The effect of aging on vasopressin (VP) clearance was studied in male Fischer 344 rats 2 (young), 12 (adult) and 30 (old) months of age using in situ liver perfusion technique. The liver was perfused with Krebs-Henseleit bicarbonate buffer containing 1% bovine serum albumin and VP (2 ng/ml), at a constant temperature of 37 +/- 0.5 degrees C and a flow rate of 150 ml/h for 120 min. Expressed per whole liver and per gram tissue, VP declined exponentially over the studied period fitting well to the curve y = bekx (p = 0.0001) in all three groups. The analysis of covariance revealed the slope of the VP decay curve to be steepest in old rats, significantly different from that of young (p = 0.0001 expressed per whole liver or per gram tissue) and adult (p = 0.0001 and p = 0.001, expressed per whole liver and per gram tissue, respectively) rats. This suggests that, at least in male Fischer 344 rats, the hepatic clearance of VP was fastest in old rats.
Shih
A P
AP
UMD-New Jersey Medical School, Department of Anesthesiology, Newark.
Zbuzek
V K
VK
Sauchelli
F C
FC
Wu
W H
WH
D000375:Aging / Q000378:metabolism*; D000818:Animals; D008099:Liver / Q000378:metabolism*; D008297:Male; D010477:Perfusion; D051381:Rats; D011916:Rats, Inbred F344; D014667:Vasopressins / Q000378:metabolism*; D014882:Water-Electrolyte Balance
D014667:Vasopressins