title: Complete nucleotide sequence of pAL5000, a plasmid from Mycobacterium fortuitum.
abstract: We have determined the complete nucleotide sequence of pAL5000, a plasmid from Mycobacterium fortuitum; the plasmid contains 4837 bp with 65% G + C. Five open reading frames (ORF1 to ORF5) have been identified. A number of sequences corresponding to palindromes, repeats, a helix-turn-helix motif, a signal sequence and repetitive amino acid motifs can be identified. This sequence should facilitate the construction of vectors based on pAL5000 for transfer and expression studies in mycobacteria.
authors.affiliation: Unité de Génie Microbiologique, Institut Pasteur, Paris, France.
authors.forename: J
authors.initials: J
authors.lastname: Rauzier
authors.forename: J
authors.initials: J
authors.lastname: Moniz-Pereira
authors.forename: B
authors.initials: B
authors.lastname: Gicquel-Sanzey
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D001709:Biotechnology; D003001:Cloning, Molecular; D008969:Molecular Sequence Data; D009161:Mycobacterium / Q000235:genetics*; D010957:Plasmids; D015183:Restriction Mapping