G3m(21) typing by ELISA and dot immunobinding with enzyme-labeled monoclonal anti-G3m(21) antibody.
Simple, rapid methods are described for G3m(21) typing with peroxidase-labeled monoclonal anti-G3m(21) antibody. In G3m(21) typing by ELISA, microtiter wells were coated directly with the test antigen, which was detected with the enzyme-labeled monoclonal antibody. To further simplify the procedure, a dot immunobinding method was developed. The antigen in the test serum applied onto a nitrocellulose membrane was successfully detected with the enzyme-labeled monoclonal antibody. These methods, particularly the dot immunobinding, are suitable for forensic casework because they are rapid and simple and require no technical skill.
Kishida
T
T
Department of Forensic Medicine, Medical College of Oita, Japan.
Tamaki
Y
Y
Fukuda
M
M
D000911:Antibodies, Monoclonal; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D015151:Immunoblotting; D007129:Immunoglobulin Gm Allotypes / Q000235:genetics*; D007564:Japan; D010641:Phenotype
D000911:Antibodies, Monoclonal; D007129:Immunoglobulin Gm Allotypes