ADP-ribosyl proteins formed by pertussis toxin are specifically cleaved by mercury ions.
Various types of ADP-ribosyl protein conjugates were synthesized and their chemical stability was compared with that of cysteine-linked ADP-ribosyl groups as formed by incubation of transducin or Gi/Go proteins with NAD and pertussis toxin. Treatment with 0.1 mM HgCl2 specifically cleaved the cysteine-linked conjugates. This may provide a tool for the quantitation of modified Gi/Go proteins as well as of other acceptors modified by ADP-ribose at cysteine residues in the presence of other ADP-ribosyl proteins.
Meyer
T
T
Institut für Physiologische Chemie, Universität Hamburg, Germany.
Koch
R
R
Fanick
W
W
Hilz
H
H
D000246:Adenosine Diphosphate Ribose / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D003545:Cysteine; D006657:Histones / Q000378:metabolism*; D006868:Hydrolysis; D008628:Mercury / Q000494:pharmacology*; D008929:Mitochondria, Heart / Q000378:metabolism; D009243:NAD / Q000378:metabolism; D020652:Peptide Elongation Factor 2; D010445:Peptide Elongation Factors / Q000378:metabolism; D010455:Peptides / Q000378:metabolism*; D037342:Pertussis Toxin; D011506:Proteins / Q000378:metabolism*; D013367:Submitochondrial Particles / Q000378:metabolism; D015293:Transducin / Q000378:metabolism; D010566:Virulence Factors, Bordetella / Q000378:metabolism*
D006657:Histones; D020652:Peptide Elongation Factor 2; D010445:Peptide Elongation Factors; D010455:Peptides; D011506:Proteins; D010566:Virulence Factors, Bordetella; D009243:NAD; D000246:Adenosine Diphosphate Ribose; D037342:Pertussis Toxin; D015293:Transducin; D008628:Mercury; D003545:Cysteine