title: Regulation of a murine macrophage haemagglutinin (sheep erythrocyte receptor) by a species-restricted serum factor.
abstract: The mechanisms which generate heterogeneity amongst resident tissue macrophages (M phi) are poorly understood. In a previous study we described a novel mouse M phi haemagglutinin, which binds unopsonized sheep erythrocytes. This sheep erythrocyte receptor (SER) is expressed at high levels on stromal M phi from bone marrow and lymph nodes, but at low levels on M phi from serous cavities and broncho-alveolar spaces. In this paper we demonstrate that a species-restricted factor in mouse serum is required in vitro for optimal maintenance of SER on resident bone marrow M phi and for its induction on M phi populations which normally lack this receptor. Using thioglycollate-elicited peritoneal M phi, induction of SER by mouse serum was dose-dependent, reached maximal levels by 3-4 days, required the continuous presence of mouse serum, and was fully reversible. Re-expression following trypsinization was inhibited by cycloheximide, showing that protein synthesis by M phi was necessary. Using a quantitative microtitre plate assay to measure levels of the inducing activity (SER-IA) in different samples, it was found to be heat-labile, non-dialysable, precipitable by polyethylene glycol and inactivated at pH 4 but not at pH 9.6. On gel filtration of mouse serum, a single major peak of activity was obtained with an apparent MW of around 70,000. SER-IA appears to be unrelated to a variety of factors and cytokines which affect M phi function, including colony-stimulating factor-1 (CSF-1). The possible role of SER-IA in regulating the differential expression of SER in vivo is discussed.
authors.affiliation: Sir William Dunn School of Pathology, University of Oxford, U.K.
authors.forename: P R
authors.initials: PR
authors.lastname: Crocker
authors.forename: M
authors.initials: M
authors.lastname: Hill
authors.forename: S
authors.initials: S
authors.lastname: Gordon
mesh_terms: D000818:Animals; D001202:Ascitic Fluid / Q000276:immunology; D001769:Blood; D001853:Bone Marrow / Q000276:immunology; D002478:Cells, Cultured; D005260:Female; D006388:Hemagglutinins / Q000032:analysis*; D008264:Macrophages / Q000276:immunology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D051381:Rats; D011919:Rats, Inbred Strains; D011971:Receptors, Immunologic / Q000032:analysis*; D012397:Rosette Formation; D013045:Species Specificity
chemical_list: D006388:Hemagglutinins; D011971:Receptors, Immunologic