Cytochemical methods for the localization of invertase activity in plant tissues.
Two cytochemical methods for the localization of acid and alkaline invertases are given. The first is based upon the reduction of a silver complex at two different pH ranges, whilst the second is based upon the tetrazolium reaction and permits quantification of the rate of activity of alkaline invertase activity. The distribution of alkaline invertase activity throughout the root apex of Pisum sativum and the cell wall localization of acid invertase for material excised from tuber tissue of Helianthus tuberosus are both confirmed.
Chapman
J
J
Department of Biology, King's College, London, England.
McGarry
A
A
Carmignac
D F
DF
Gahan
P D
PD
D006026:Glycoside Hydrolases / Q000032:analysis*; D006651:Histocytochemistry / Q000379:methods*; D009580:Nitroblue Tetrazolium; D010944:Plants / Q000201:enzymology*; D012835:Silver Nitrate; D043324:beta-Fructofuranosidase
D009580:Nitroblue Tetrazolium; D012835:Silver Nitrate; D006026:Glycoside Hydrolases; D043324:beta-Fructofuranosidase