title: Circular dichroism of isolated ricin A- and B-chains.
abstract: An analysis of the circular dichroism (CD) spectra of isolated ricin A- and B-chains revealed several bands not apparent in the spectrum of intact ricin. Arithmetic combination of the A- and B-chain spectra gave a composite spectrum resembling that of native ricin, indicating that the two chains did not undergo any major conformational change upon dissociation. The addition of lactose to the B-chain at pH 7.2 caused a slight perturbation of a tryptophan-derived negative CD band centred at 283 nm without change to the overall structure of the polypeptide.
authors.affiliation: Drug Targeting Laboratory, Imperial Cancer Research Fund Laboratories, London, U.K.
authors.forename: E J
authors.initials: EJ
authors.lastname: Wawrzynczak
authors.forename: A F
authors.initials: AF
authors.lastname: Drake
authors.forename: P E
authors.initials: PE
authors.lastname: Thorpe
mesh_terms: D002942:Circular Dichroism; D046911:Macromolecular Substances; D011487:Protein Conformation; D012276:Ricin
chemical_list: D046911:Macromolecular Substances; D012276:Ricin