Interparticle interactions and structural changes of nucleosome core particles in low-salt solution.
The structural behavior of the nucleosome core particles in the range of solvent Na+ concentration from 10.45 to 0.45 mM has been studied by small-angle neutron and synchroton radiation X-ray scattering, sedimentation, atomic absorption spectroscopy, density measurements, and circular dichroism. With decreasing salt concentration, the appearance of a scattering peak that is assignable to interparticle interactions, an intraparticle structural transition, a decrease in the sedimentation velocity of the particle, and a release of bound Na+ ions from the particle are all observed concurrently when the ratio of solvent Na+ ions per particle is below approximately 1000. These observations are interpreted to indicate that a release of bound Na+ ions from the particle brings about structural rearrangements and weakens the electrostatic shielding of the particle, and this introduces long-range repulsive ordering of the particle in low-salt solution. Analyses of the scattering data indicate that the rearrangement within the core particle in low-salt solution is slight, changing the particle's shape slightly from cylindrical to a more spherical form by moving the center of the mass of the DNA somewhat inward with accompanying small decreases in the radii of gyration of both the DNA and the histones.
Hirai
M
M
Kanagawa Institute of Technology, Atsugi, Japan.
Niimura
N
N
Zama
M
M
Mita
K
K
Ichimura
S
S
Tokunaga
F
F
Ishikawa
Y
Y
D000818:Animals; D002645:Chickens; D002942:Circular Dichroism; D004247:DNA / Q000378:metabolism; D006657:Histones / Q000378:metabolism; D066298:In Vitro Techniques; D009707:Nucleosomes / Q000378:metabolism*; D009994:Osmolar Concentration; D011487:Protein Conformation; D012542:Scattering, Radiation; D012965:Sodium Chloride; D012996:Solutions; D013054:Spectrophotometry, Atomic
D006657:Histones; D009707:Nucleosomes; D012996:Solutions; D012965:Sodium Chloride; D004247:DNA