Characteristic structures of actin gels induced with hepatic actinogelin or with chicken gizzard alpha-actinin: implication for their function.
We studied the properties of actinogelin, a Ca2+-regulated actin cross-linking protein isolated from Ehrlich tumor cells or rat liver. Chicken gizzard alpha-actinin was used as a Ca2+-insensitive control. Actinogelin, which has very high gelation activity under low Ca2+ conditions, was found using electron microscopic or fluorescence studies to induce formation of a characteristic structure in which actin filaments and bundles radiate to (or converge from) all directions from spot-like core structures. A similar structure was induced with actinogelin, even in the presence of 0.7 saturation of tropomyosin. No such structure was detected with actinogelin under high Ca2+ conditions, and only a few were found with gizzard alpha-actinin. Because reconstituted structures are similar to those observed intracellularly, actinogelin may be important in the formation of similar microfilament organization in the cells. It seems also important that these structures are reconstituted with only two purified protein components, i.e., actinogelin and actin. Immunocompetition studies showed that actinogelin and gizzard alpha-actinin partially shared antigenicity, and their molecular shape and peptide maps were similar. Their amino acid compositions [Kuo et al., 1982], subunit and domain structures, and binding sites on actin [Mimura and Asano, 1987] are also very similar. Therefore, it is concluded that actinogelin belongs to alpha-actinin superfamily proteins. Furthermore, the presence of functionally different subfamilies concerned with Ca2+ sensitivity, gelation-efficiency, and others is discussed. Actinogelin, which induces networks of actin filaments, may be classified as high gelation type.
Tsukita
S
S
Tokyo Metropolital Institute of Medical Science, Japan.
Mimura
N
N
Tsukita
S
S
Khono
K
K
Ohtaki
T
T
Oshima
T
T
Ishikawa
H
H
Asano
A
A
D000185:Actinin / Q000032:analysis / Q000276:immunology / Q000494:pharmacology* / Q000502:physiology; D000199:Actins / Q000032:analysis / Q000502:physiology; D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D002642:Chick Embryo; D002645:Chickens; D003429:Cross Reactions; D005347:Fibroblasts / Q000032:analysis / Q000648:ultrastructure; D005782:Gels; D005895:Gizzard, Avian / Q000032:analysis*; D008099:Liver / Q000032:analysis*; D008840:Microfilament Proteins / Q000032:analysis / Q000276:immunology / Q000494:pharmacology* / Q000502:physiology; D008854:Microscopy, Electron; D015394:Molecular Structure; D009210:Myofibrils / Q000032:analysis; D051381:Rats
D000199:Actins; D005782:Gels; D008840:Microfilament Proteins; C029354:actinogelin; D000185:Actinin