title: Comparison of the dynamics of the membrane-bound form of fd coat protein in micelles and in bilayers by solution and solid-state nitrogen-15 nuclear magnetic resonance spectroscopy.
abstract: Solid-state and solution 15N nuclear magnetic resonance experiments on uniformly and specifically 15N labeled coat protein in phospholipid bilayers and in detergent micelles are used to describe the dynamics of the membrane-bound form of the protein. The residues in the N- and C-terminal portions of the coat protein in both phospholipid bilayers and in detergent micelles are mobile, while those in the hydrophobic midsection are immobile. There is evidence for a gradient of mobility in the C-terminal region of the coat protein in micelles; at 25 degrees C only the last two residues are mobile on the 10(9)-Hz timescale, while the last six to eight residues appear to be mobile on slower timescales and highly mobile at higher temperatures. Since all of the C-terminal residues are immobile in the virus particles, the mobility of these residues in the membrane-bound form of the protein may be important for the formation of protein-DNA interactions in the assembly process.
authors.affiliation: Department of Chemistry, University of Pennsylvania, Philadelphia 19104.
authors.forename: M J
authors.initials: MJ
authors.lastname: Bogusky
authors.forename: G C
authors.initials: GC
authors.lastname: Leo
authors.forename: S J
authors.initials: SJ
authors.lastname: Opella
mesh_terms: D015060:1,2-Dipalmitoylphosphatidylcholine; D003102:Colloids; D004268:DNA-Binding Proteins; D004134:Dimyristoylphosphatidylcholine; D008051:Lipid Bilayers; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008823:Micelles; D009587:Nitrogen Isotopes; D011487:Protein Conformation; D014764:Viral Proteins
chemical_list: D003102:Colloids; D004268:DNA-Binding Proteins; D008051:Lipid Bilayers; D008823:Micelles; D009587:Nitrogen Isotopes; D014764:Viral Proteins; C057816:gene 5 protein, Enterobacteria phage M13; D015060:1,2-Dipalmitoylphosphatidylcholine; D004134:Dimyristoylphosphatidylcholine