title: Formation of a new fibrous attachment to human dental roots. A new vitro model for studying periodontal regeneration.
abstract: This study was performed to improve currently employed in vitro models for the study of periodontal regeneration by using a porous filter upon which periodontal ligament cells were grown. Periodontal ligament cells were harvested and 0.3 mm root discs cut from three partially erupted and extracted third molar teeth of one patient. Experimental culturing was performed by seeding periodontal ligament cell suspensions on Puropor-200 filters supported by wire-mesh grids in Grobstein Petri dishes. The following day, an interdental space of 0.1 to 0.3 mm was created by gently placing two dental root discs upon the filter. Cultures were terminated after 42, 56, 112 and 124 days, and processed for light- and electron microscopy. Collagen fibril diameters were measured. Adjacent and often attached to large areas of cementum-lined root discs, a dense fiber fringe developed. This fiber fringe was not found on dentin-lined root discs. Although less organized, older cultures demonstrated a similar disc-culture interface, which depended upon the presence or absence of original root cementum. Collagen fibrils of early cultures had a mean diameter of about 42 nm, while in older cultures the diameters ranged from 47 to 68 nm. It is concluded that the fibrous matrix attached to cementum-lined root discs somewhat resembles the initial stages of the formation of dental root cementum in vivo.
authors.affiliation: Department of Oral Structural Biology, University of Zürich, Switzerland.
authors.forename: A B
authors.initials: AB
authors.lastname: Bernstein
authors.forename: E
authors.initials: E
authors.lastname: Preisig
authors.forename: H E
authors.initials: HE
authors.lastname: Schroeder
mesh_terms: D000328:Adult; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D008854:Microscopy, Electron; D010513:Periodontal Ligament / Q000166:cytology / Q000648:ultrastructure; D012038:Regeneration; D014092:Tooth Root / Q000502:physiology*