title: Determination of the N-acetyl metabolites of 4,4'-methylene dianiline and 4,4'-methylene-bis(2-chloroaniline) in urine.
abstract: A sensitive and specific gas chromatographic/mass spectrometric assay is described for the determination of N-acetyl 4,4'-methylene dianiline (N-acetyl MDA) and N-acetyl 4,4'-methylene-bis(2-chloroaniline) (N-acetyl MbOCA) in urine. The method is based on the solvent extraction of the compounds together with deuterium-labelled internal standards, the compounds being separated and detected by capillary gas chromatography/mass spectrometry as their pentafluoropropyl derivatives. The method has been applied to the detection of N-acetyl MbOCA and N-acetyl MDA in the urine of workers occupationally exposed to MbOCA and MDA. The results show that whilst N-acetyl MbOCA is a relatively minor urinary metabolite a significant proportion of MDA is excreted as the N-acetylated compound.
authors.affiliation: Occupational Medicine and Hygiene Laboratories, Health and Safety Executive, London, UK.
authors.forename: J
authors.initials: J
authors.lastname: Cocker
authors.forename: A R
authors.initials: AR
authors.lastname: Boobis
authors.forename: D S
authors.initials: DS
authors.lastname: Davies
mesh_terms: D000083:Acetanilides / Q000652:urine*; D001559:Benzhydryl Compounds / Q000652:urine*; D004781:Environmental Exposure; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D008753:Methylenebis(chloroaniline) / Q000031:analogs & derivatives / Q000652:urine*
chemical_list: D000083:Acetanilides; D001559:Benzhydryl Compounds; C050075:N-acetyl-4,4'-diaminodiphenylmethane; D008753:Methylenebis(chloroaniline); C058419:N-acetyl-4,4'-methylenebis(2-chloroaniline)