Differential location of regulatory and nonregulatory trehalases in Candida utilis cells.
The isolation of vacuoles by density gradient centrifugation of protoplast lysates from Candida utilis cells showed a high specific activity for nonregulatory trehalase in vacuoles whereas the regulatory trehalase activatable by phosphorylation behaves as a cytoplasmic enzyme. The vacuolar trehalase is a glycoprotein that can be precipitated by Con A-Sepharose. Treatment of this enzyme with endo H reduced its reactivity with the lectin without loss of enzyme activity and decreased its apparent molecular weight by gel filtration.
Arguelles
J C
JC
Department of Microbiology, Facultad de Biología, Universidad de Murcia, Spain.
Gacto
M
M
D002175:Candida / Q000201:enzymology* / Q000502:physiology / Q000648:ultrastructure; D014198:Trehalase / Q000032:analysis* / Q000145:classification / Q000502:physiology; D014617:Vacuoles / Q000201:enzymology / Q000502:physiology / Q000648:ultrastructure
D014198:Trehalase