title: Lymphocyte phenotypes in the abomasal mucosa of sheep infected with Haemonchus contortus.
abstract: Lymphocyte subpopulations in the abomasal mucosa of worm-free and parasitized sheep were assessed in situ. A preponderance of T-lymphocytes, with approximately equal numbers of cells expressing CD5, CD4 and CD8 antigens, was found. Most of the lymphocytes expressing CD8 lacked CD5. Using a panel of 15 monoclonal antibodies to ovine leucocyte antigens, abomasal lymphoid follicles in the mucosa were shown to resemble lymph node follicles phenotypically. Abomasal epithelial cells contained major histocompatibility complex (MHC) class II antigen. Infection or hyperimmunization of pasture-reared sheep with the gastric nematode Haemonchus contortus increased the numbers of mucosal mast cells and eosinophils but did not alter the phenotypic composition or number of mucosal lymphocytes or the pattern of expression of MHC class II antigens.
authors.affiliation: Department of Veterinary Preclinical Sciences, University of Melbourne, Parkville, Victoria, Australia.
authors.forename: M D
authors.initials: MD
authors.lastname: Gorrell
authors.forename: H R
authors.initials: HR
authors.lastname: Miller
authors.forename: M R
authors.initials: MR
authors.lastname: Brandon
mesh_terms: D000018:Abomasum / Q000276:immunology* / Q000473:pathology; D000818:Animals; D000954:Antigens, Surface; D005260:Female; D005753:Gastric Mucosa / Q000276:immunology / Q000473:pathology; D006188:Haemonchiasis / Q000276:immunology / Q000473:pathology / Q000662:veterinary*; D000949:Histocompatibility Antigens Class II; D008214:Lymphocytes / Q000145:classification / Q000276:immunology* / Q000473:pathology; D008297:Male; D012756:Sheep; D012757:Sheep Diseases / Q000276:immunology* / Q000473:pathology; D014252:Trichostrongyloidiasis / Q000662:veterinary*
chemical_list: D000954:Antigens, Surface; D000949:Histocompatibility Antigens Class II