Determination of verapamil and its primary metabolites in serum by ion-pair adsorption high-performance liquid chromatography.
A high-performance liquid chromatographic (HPLC) method for the simultaneous quantitation of verapamil, norverapamil, N-dealkylverapamil (D617) and N-dealkylnorverapamil (D620) concentrations in serum is developed. Analysis is performed on a microparticulate (10 microns) silica column using a counter-ion solvent system (0.6 mM NaBr in methanol). Column effluent is monitored by fluorescence detection at an excitation wavelength of 203 nm. The limit of sensitivity is less than 1 ng for all compounds in serum. No potential sources of interference are identified and a coefficient of variation of less than 10% is observed on replicate verapamil determinations. The method has the advantages of complete resolution of the metabolites of verapamil, low limits of detection, high degree of reproducibility, and short analysis time.
Pieper
J A
JA
Department of Clinical Pharmacy, College of Pharmacy, University of Tennessee, Memphis 38163.
Rutledge
D R
DR
D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007202:Indicators and Reagents; D014700:Verapamil / Q000031:analogs & derivatives* / Q000097:blood* / Q000493:pharmacokinetics
D007202:Indicators and Reagents; C016906:alpha-(3-aminopropyl)-3,4-dimethoxy-alpha-(1-methylethyl)benzeneacetonitrile; D014700:Verapamil