Differential expression of five tRNA(UAGTrp) amber suppressors in Caenorhabditis elegans.
Caenorhabditis elegans has 12 tRNA(UGGTrp) genes as defined by Southern analysis. In order to evaluate the function of the individual members of this multigene family, we sought to recover amber (UAG)-suppressing mutations from reversion experiments with animals carrying amber mutations in a nervous system-affecting gene (unc-13) or a sex-determining gene (tra-3). Revertants were analyzed by Southern blot, exploiting the fact that the CCA to CTA change at the anticodon creates a new XbaI site. Five different members of the tRNATrp gene family were identified as suppressors: sup-7 X, sup-5 III, sup-24 IV, sup-28 X, and sup-29 IV. All five suppressor genes were sequenced and found to encode identical tRNA(UAGTrp) molecules with a single base change (CCA to CTA) at the anticodon compared with their wild-type counterparts. The flanking sequences had only limited homology. The relative expression of these five genes was determined by measuring the efficiencies of suppressers against amber mutations in genes affecting the nervous system, hypodermis, muscle, and sex determination. The results of these cross-suppression tests showed that the five members of the tRNA(Trp) gene family were differentially regulated in a tissue- or development stage-specific manner.
Kondo
K
K
Department of Genetics, Washington University School of Medicine, St. Louis, Missouri 63110.
Hodgkin
J
J
Waterston
R H
RH
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002107:Caenorhabditis / Q000235:genetics*; D003001:Cloning, Molecular; D003062:Codon; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D012346:RNA, Transfer, Amino Acyl / Q000235:genetics*; D013489:Suppression, Genetic; D014158:Transcription, Genetic
D003062:Codon; D012346:RNA, Transfer, Amino Acyl; D004247:DNA