title: Substrate effects on the dynamics of neurite growth in vitro: a quantitative multi-parametric analysis.
abstract: Embryonic chick dorsal root ganglia were cultured in serum-free medium on natural (collagen, fibronectin and hyaluronic acid) and artificial (polylysine and polyornithine) substrata. The movement of individual growth cones was quantified by measuring five parameters using time-lapse cinematography combined with a digitizing-computer system, and the neurite behaviour was compared between the different substrata with multivariate statistical methods. For each substratum, the morphometry of the growth cone was quantified by measuring six morphological parameters. The most discriminative parameters proved to be mean velocity and straightness index for neurite extension, and projected area and cumulated length of filopodia for growth cone morphometry. A good correlation was obtained between behavioural and morphological parameters and the larger the cone area and the filopodia length, the faster and the straighter the neuritic growth. Both quantitative analyses showed highest values for polyornithine and the lowest for hyaluronic acid, and divided the substrata into two opposite groups, artificial and natural. It is concluded that growth cone behaviour and conformation is modulated by substratum properties.
authors.affiliation: Unité de Morphogenèse Expérimentale, UA, CNRS 682, Université de Grenoble I, France.
authors.forename: R
authors.initials: R
authors.lastname: Saxod
authors.forename: M C
authors.initials: MC
authors.lastname: Bizet
mesh_terms: D000818:Animals; D002642:Chick Embryo; D003094:Collagen / Q000494:pharmacology; D046508:Culture Techniques; D005353:Fibronectins / Q000494:pharmacology; D005727:Ganglia, Spinal / Q000166:cytology / Q000187:drug effects / Q000196:embryology*; D006820:Hyaluronic Acid / Q000494:pharmacology; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D010455:Peptides / Q000494:pharmacology; D011107:Polylysine / Q000494:pharmacology
chemical_list: D005353:Fibronectins; D010455:Peptides; C008973:polyornithine; D011107:Polylysine; D006820:Hyaluronic Acid; D003094:Collagen