The effects of prostaglandin E1 on megakaryocyte proliferation in vitro.
A concentration of 560 nM Prostaglandin E1 (PGE1), in a medium containing either 10% serum and 10% plasma, or 20% plasma and a platelet extract prepared from 24 x 10(6) platelets/ml, caused after four days of incubation in a plasma clot culture system, highly significant increases over the controls in both the number of megakaryocyte colonies/clot and megakaryocytes/clot. A linear dose-response relationship over the entire 14-560 nM range was also demonstrated in cultures containing serum, between the concentration of PGE1 in each culture, and the number of megakaryocyte colonies/clot and megakaryocytes/clot. However, PGE1 did not appear to stimulate megakaryocytes in cultures containing only 20% rat plasma.
Cooper
G W
GW
Department of Biology, City College, City University of New York, NY 10031.
Hou
X P
XP
D000527:Alprostadil / Q000494:pharmacology*; D000818:Animals; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D003470:Culture Media; D006133:Growth Substances / Q000097:blood; D006410:Hematopoiesis / Q000187:drug effects*; D008533:Megakaryocytes / Q000502:physiology*; D051381:Rats; D013154:Spleen / Q000166:cytology
D003470:Culture Media; D006133:Growth Substances; D000527:Alprostadil