title: In vivo 31P NMR studies of rat salivary glands at 6.3 tesla.
abstract: In vivo 31P NMR spectra of rat submandibular glands were obtained. The glands were exposed, leaving the neurovascular system intact, and placed in a solenoidal coil. Resonances of nine phosphate metabolites were identified in the spectra and metabolite concentrations were estimated from the corresponding integrals ([ATP] = 3.4 +/- 0.7 mM). Tissue pH, as deduced from the chemical shift of the inorganic phosphate resonance, was 7.26 (+/- 0.07). T1 relaxation times of ATP, phosphocreatine, inorganic phosphate, and phosphomonoester 31P spin systems were examined. The effect of hypoxia was followed as a function of time. 31P NMR spectra of the glands have also been obtained noninvasively by the use of a surface coil, adapted to the dimensions of the glands, and depth pulses.
authors.affiliation: Philips Research Laboratories, Eindhoven, The Netherlands.
authors.forename: A
authors.initials: A
authors.lastname: Heerschap
authors.forename: J J
authors.initials: JJ
authors.lastname: van Vaals
authors.forename: A H
authors.initials: AH
authors.lastname: Bergman
authors.forename: J H
authors.initials: JH
authors.lastname: den Boef
authors.forename: P H
authors.initials: PH
authors.lastname: van Gerwen
authors.forename: E J
authors.initials: EJ
authors.lastname: Gravenmade
mesh_terms: D000818:Animals; D004734:Energy Metabolism; D000860:Hypoxia / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013363:Submandibular Gland / Q000378:metabolism*