title: Anti-HIV antibodies in the CSF of AIDS patients: a serological and immunoblotting study.
abstract: CSF and serum samples from 16 AIDS patients were tested for the presence of anti-HIV antibodies either by classical serological methods or by an immunoblot technique based on agarose gel isoelectric focusing and transfer of the specific IgG antibodies onto HIV antigens-loaded nitrocellulose sheets. This method enabled the demonstration of an intrathecal synthesis of anti-HIV oligoclonal IgG antibodies, often superimposed on diffuse polyclonal production, in 14 patients. The two negative cases were devoid of neurological signs or symptoms. However, two patients classified in stage II of the disease (asymptomatic infection) displayed an intrathecal synthesis of anti-HIV antibodies.
authors.affiliation: Department of Neurology, St-Luc Hospital, University of Louvain, Brussels, Belgium.
authors.forename: K S
authors.initials: KS
authors.lastname: Bukasa
authors.forename: C J
authors.initials: CJ
authors.lastname: Sindic
authors.forename: M
authors.initials: M
authors.lastname: Bodeus
authors.forename: G
authors.initials: G
authors.lastname: Burtonboy
authors.forename: C
authors.initials: C
authors.lastname: Laterre
authors.forename: J
authors.initials: J
authors.lastname: Sonnet
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000175:diagnosis* / Q000276:immunology; D000328:Adult; D000368:Aged; D003704:Dementia / Q000175:diagnosis* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006678:HIV / Q000276:immunology*; D015483:HIV Antibodies / Q000134:cerebrospinal fluid*; D006801:Humans; D015151:Immunoblotting; D007074:Immunoglobulin G / Q000134:cerebrospinal fluid; D008297:Male; D008875:Middle Aged
chemical_list: D015483:HIV Antibodies; D007074:Immunoglobulin G