The expression of insulin-like growth factor I and insulin-like growth factor II genes in the human fetal and adult brain and in glioma.
Expression of insulin-like growth factor I (IGF-I) and insulin-like growth factor II (IGF-II) genes in the human brain and in glioma was studied by slot blot and Northern analysis following hybridization of IGF-I and IGF-II cDNA probes to isolated mRNA. In the fetal brain there were major IGF-I transcripts of 7.5 and 4.4 kilobases (kb) and one major IGF-II transcript of 6.0 kb. IGF-II mRNA was less abundant in the adult brain, with IGF-II mRNA being scarcely detectable. All glioma tissue examined displayed a marked enhancement of both IGF-I and IGF-II gene expression when compared to normal brain. IGF-I transcripts of 4.4 and 2.2-1.8 kb were identified. Similar to fetal brain a major IGF-II transcript of 6.0 kb was found in the glioblastoma examined.
Sandberg
A C
AC
Karolinska Institute's Department of Psychiatry, St. Göran's Hospital, Stockholm, Sweden.
Engberg
C
C
Lake
M
M
von Holst
H
H
Sara
V R
VR
D001921:Brain / Q000196:embryology / Q000378:metabolism*; D001932:Brain Neoplasms / Q000378:metabolism*; D005786:Gene Expression Regulation; D005865:Gestational Age; D005910:Glioma / Q000378:metabolism*; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000235:genetics* / Q000378:metabolism; D007335:Insulin-Like Growth Factor II / Q000235:genetics* / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000378:metabolism; D013002:Somatomedins / Q000235:genetics*
D012333:RNA, Messenger; D013002:Somatomedins; D007334:Insulin-Like Growth Factor I; D007335:Insulin-Like Growth Factor II