Discriminant analysis of microcalorimetric data of bacterial growth.
In this work a bacterial classification method based on the discriminant analysis of the microcalorimetric data provided by the growth power-time (p-t) curves is developed. This method is applied to classify several species of Enterobacteria of different origins, and the results are compared with those obtained by conventional techniques. The proposed analysis allows us to classify bacteria into species and discriminate among strains of the same species. The classification is carried out using one run of each isolate after standardization of inocula and growth conditions. The discrimination power of available microcalorimetric data is also discussed, and the most discriminant set of data is proposed as the input variables of the analysis. Finally, the advantages of microcalorimetry as a taxonomical technique are discussed.
Bermúdez
J
J
Departament Ciencies Fisiològiques Humanes i de la Nutrició, Facultat de Medicina, Universitat de Barcelona, Spain.
López
D
D
Sanahuja
A
A
Viñas
M
M
Lorén
J G
JG
D002151:Calorimetry; D004755:Enterobacteriaceae / Q000254:growth & development* / Q000378:metabolism; D008433:Mathematics