Strategy for obtaining non-native protein structures using antibody cross-reactions.
Antibodies made to short peptides or to unfolded forms of proteins are often found to cross-react with intact proteins. These cross-reactions can be used to populate non-native protein conformations, possibly including protein folding intermediates, and the structures of the non-native conformations can be characterized using amide proton exchange and two-dimensional NMR.
Kim
P S
PS
Whitehead Institute for Biomedical Research, Cambridge, MA 02142.
D000906:Antibodies; D000936:Antigen-Antibody Complex; D003429:Cross Reactions; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D011487:Protein Conformation; D011506:Proteins / Q000276:immunology
D000906:Antibodies; D000936:Antigen-Antibody Complex; D011506:Proteins