[Determination of biogenic amines by RP-HPLC for monitoring the microbial spoilage of poultry].
A simple high-performance liquid chromatographic (HPLC) analysis is described for the determination of biogenic amines in broiler carcasses. The clean-up procedure consists of an extraction with 0.6 M-perchloric acid, formation of dansyl derivatives, separation by a RP-18 column and UV detection at 254 nm. Within 7 min eight amines could be separated. The quantitative determination of spermidine and spermine requires an additional ion-exchange clean-up with Amberlite CG 50 after the extraction. This procedure gives recoveries of 82%-96% with detection limits of 0.2-0.5 microgram/g of broiler skin. Putrescine and cadaverine are good indicators for the onset of spoilage of poultry carcasses, since both amines could be detected at total colony counts of 10(5) cfu/cm2 and their concentration increases rapidly with advancing decomposition.
Schmitt
R E
RE
Institut für Lebensmittelwissenschaft, ETH Zentrum, Zürich, Schweiz.
Haas
J
J
Amadò
R
R
D000818:Animals; D001679:Biogenic Amines / Q000032:analysis*; D002103:Cadaverine / Q000032:analysis; D002645:Chickens; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005506:Food Contamination / Q000032:analysis; D005516:Food Microbiology; D007202:Indicators and Reagents; D008460:Meat / Q000032:analysis*; D011700:Putrescine / Q000032:analysis; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
D001679:Biogenic Amines; D007202:Indicators and Reagents; D002103:Cadaverine; D011700:Putrescine
Bestimmung von biogenen Aminen mit RP-HPLC zur Erfassung des mikrobiellen Verderbs von Schlachtgeflügel.