title: Rapid and non-radioactive prenatal diagnosis of beta thalassaemia and sickle cell disease: application of the polymerase chain reaction (PCR).
abstract: The standard method for the prenatal diagnosis of the haemoglobinopathies is by restriction enzyme mapping of chorionic villus DNA using Southern blotting and radioactively labelled gene probes. An improvement of the procedure which involves the selective amplification of DNA fragments by the polymerase chain reaction allows one to visualize restriction fragments directly without the use of radioactivity and within 2 d after obtaining the sample. We report here the prenatal diagnosis of two pregnancies at risk for homozygous beta thalassaemia and homozygous sickle cell disease using this novel approach.
authors.affiliation: Department of Paediatrics II, University of Ulm, F.R.G.
authors.forename: A E
authors.initials: AE
authors.lastname: Kulozik
authors.forename: J
authors.initials: J
authors.lastname: Lyons
authors.forename: E
authors.initials: E
authors.lastname: Kohne
authors.forename: C R
authors.initials: CR
authors.lastname: Bartram
authors.forename: E
authors.initials: E
authors.lastname: Kleihauer
mesh_terms: D000755:Anemia, Sickle Cell / Q000175:diagnosis* / Q000235:genetics; D004259:DNA-Directed DNA Polymerase; D005260:Female; D005914:Globins / Q000235:genetics*; D006801:Humans; D007223:Infant; D008297:Male; D011296:Prenatal Diagnosis / Q000379:methods*; D015183:Restriction Mapping; D013789:Thalassemia / Q000175:diagnosis* / Q000235:genetics
chemical_list: D005914:Globins; D004259:DNA-Directed DNA Polymerase