title: Production and characterization of a monoclonal antibody cross-reactive with most group A trichothecenes.
abstract: A monoclonal antibody cross-reactive with most group A trichothecenes was produced by fusion of P3/NS-1/1-AG4-1 myeloma cells with spleen cells isolated from a BALB/c mouse that had been immunized with 3-acetyl-neosolaniol-hemisuccinate conjugated to bovine serum albumin. One stable clone, H159B1D5, which produced monoclonal antibody that bound with both T-2 toxin and diacetoxyscirpenol (DAS) was obtained after subcloning. Enzyme-linked immunosorbent assay (ELISA) revealed that the antibody belongs to the immunoglobulin G1 (kappa chain) isotype and had binding constants of 2.81 x 10(9), 1.05 x 10(9), and 1.57 x 10(8) liters per mole for T-2 tetraol tetraacetate, T-2 toxin, and DAS, respectively. The relative cross-reactivities of the antibody with T-2 tetraol tetraacetate, T-2 toxin, and DAS were 200, 100, and 20, respectively, with tritiated T-2 toxin as the marker ligand. The relative cross-reactivities for the above toxins were 667, 100, and 73, respectively, with tritiated DAS as the marker ligand. No cross-reaction with HT-2 and deoxynivalenol triacetate was observed in either system. By using this monoclonal antibody, an indirect ELISA for analysis of T-2 toxin was also developed. The linear portion of the standard curve for analysis of T-2 toxin in each analysis by radioimmunoassay and ELISA was in the range of 0.1 to 2 ng and 0.05 to 1.0 ng, respectively.
authors.affiliation: Food Research Institute, University of Wisconsin-Madison 53706.
authors.forename: T S
authors.initials: TS
authors.lastname: Fan
authors.forename: S L
authors.initials: SL
authors.lastname: Schubring
authors.forename: R D
authors.initials: RD
authors.lastname: Wei
authors.forename: F S
authors.initials: FS
authors.lastname: Chu
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000918:Antibody Specificity; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D051379:Mice; D011863:Radioimmunoassay; D012717:Sesquiterpenes / Q000276:immunology*; D013605:T-2 Toxin / Q000276:immunology; D014255:Trichothecenes / Q000145:classification / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D012717:Sesquiterpenes; D014255:Trichothecenes; D013605:T-2 Toxin; C001809:diacetoxyscirpenol