title: Morphological and kinetic abnormalities of platelets in hypercholesterolemic rabbits.
abstract: Hypercholesterolemia (HC = hypercholesterolemia or hypercholesterolemic) was produced in rabbits by feeding them diets supplemented with cholesterol and peanut oil. Platelet counts and volumes, white cell counts, reticulocyte counts, and hematocrits were determined at intervals for 8-12 weeks in blood from HC animals and controls on a normal rabbit diet. Microthrombocytosis was a consistent occurrence in the presence of HC, developing as early as 2 weeks into the diet. Microthrombocytosis was generally associated with normal platelet counts, but mild thrombocytosis occurred late in the diet at the time of the highest levels of serum cholesterol (greater than 1300 mg/dl). Platelets from HC rabbits were morphologically normal by transmission electron microscopy. Survivals of 51Cr-labeled platelets from HC and non-HC rabbits were measured in HC and non-HC recipients. The results identified an intrinsic defect in the ability of HC platelets to survive in the circulation. They also confirmed previous findings of an environmental defect in HC that causes shortened platelet survival.
authors.affiliation: Lawrence Berkeley Laboratory, University of California, Berkeley 94720.
authors.forename: E
authors.initials: E
authors.lastname: Mazoyer
authors.forename: S
authors.initials: S
authors.lastname: Ebbe
authors.forename: K
authors.initials: K
authors.lastname: Dalal
authors.forename: R
authors.initials: R
authors.lastname: Leven
authors.forename: B
authors.initials: B
authors.lastname: Mazoyer
authors.forename: D
authors.initials: D
authors.lastname: Carpenter
authors.forename: T
authors.initials: T
authors.lastname: Yee
authors.forename: K
authors.initials: K
authors.lastname: Brennan
mesh_terms: D000818:Animals; D001792:Blood Platelets / Q000378:metabolism / Q000648:ultrastructure; D001803:Blood Transfusion; D002470:Cell Survival; D002784:Cholesterol / Q000097:blood; D002791:Cholesterol, Dietary / Q000008:administration & dosage; D006937:Hypercholesterolemia / Q000097:blood* / Q000209:etiology / Q000473:pathology; D008297:Male; D010976:Platelet Count; D017713:Platelet Transfusion; D011817:Rabbits; D013922:Thrombocytosis / Q000097:blood / Q000209:etiology
chemical_list: D002791:Cholesterol, Dietary; D002784:Cholesterol