Progesterone assay by inhibition of reverse passive haemagglutination.
An assay for the steroid hormone progesterone is described based on the inhibition of the agglutination by progesterone-bovine serum albumin conjugate of red cells coupled with monoclonal antiprogesterone antibody (reverse passive haemagglutination). A low-affinity agglutination system produced the optimal sensitivity, capable of detecting less than 1 ng/ml steroid. This assay has the simplicity and sensitivity for a potential clinical test for placental or corpus luteum function. It also serves as a model for detection of other small ligands (drugs, hormones) by inhibition of reverse passive haemagglutination.
Coombs
R R
RR
University Department of Pathology, Addenbrooke's Hospital, Cambridge, UK.
Kirk
D
D
Saunders
J
J
Heap
R B
RB
Taussig
M J
MJ
D000818:Animals; D000911:Antibodies, Monoclonal; D001667:Binding, Competitive; D006385:Hemagglutination Inhibition Tests / Q000379:methods; D006908:Hydroxyprogesterones / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D011374:Progesterone / Q000032:analysis* / Q000494:pharmacology; D012710:Serum Albumin, Bovine / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D006908:Hydroxyprogesterones; D012710:Serum Albumin, Bovine; D011374:Progesterone; C030250:progesterone 11-hemisuccinate