A hybrid bioartificial liver composed of multiplated hepatocyte monolayers.
Monolayer cultures of hepatocytes were shown to have good function when compared with suspended cells. The authors manufactured a new hybrid artificial liver containing hepatocyte monolayers and evaluated its function. Hepatocytes isolated from an adult dog liver were cultured on collagen coated borosilicated glass (10 X 20 X 0.04 cm). A long-stroke artificial liver module was constructed by stacking 200 glass plates bearing hepatocytes, which were viable and functioned well during 4 weeks in perfusion culture; glyconeogenesis = 110 ng/micrograms DNA/min, urea synthesis = 3.6 ng/micrograms DNA/min and albumin synthesis = 29 micrograms/10(6) cells/day at the 5th day of perfusion. The levels were maintained for 2 weeks. The new device was applied to anhepatic dogs (Group 3) and compared with untreated (Group 1) and plasma exchange dogs (Group 2). The survival times were 21.3 +/- 5.6 hours in Group 1 (N = 6), 27.8 +/- 4.0 hours in Group 2 (N = 3), and 55.0 +/- 10.3 hours in Group 3 (N = 4). The longest survival was 65 hours. Serum ammonia increased to over 2,000 micrograms/dl after 12 hours in Groups 1 and 2, but remained under 400 micrograms/dl in Group 3. This new type of hybrid system may be a pilot design for the complete artificial liver.
Uchino
J
J
First Department of Surgery, Hokkaido University School of Medicine, Tokushima University, Sapporo, Japan.
Tsuburaya
T
T
Kumagai
F
F
Hase
T
T
Hamada
T
T
Komai
T
T
Funatsu
A
A
Hashimura
E
E
Nakamura
K
K
Kon
T
T
D000818:Animals; D001187:Artificial Organs; D002478:Cells, Cultured; D004285:Dogs; D004867:Equipment Design; D006498:Hepatectomy; D008099:Liver / Q000502:physiology*; D012709:Serum Albumin / Q000378:metabolism
D012709:Serum Albumin