Cytotoxicity, sister-chromatid exchange, chromosome aberration and transformation induced by 2,2-dichlorovinyl-O,O-dimethyl phosphate.
2,2-Dichlorovinyl-O,O-dimethyl phosphate (DDVP), an extensively used household insecticide, was assayed for its genotoxicity in primary rat tracheal epithelial (RTE) cells. Cytotoxicity of DDVP to RTE cells was dose-dependent, killing about 50% of the cell population at a dose of 80 micrograms/ml. Sister-chromatid exchange (SCE) and chromosomal aberrations induced by this insecticide were positive in RTE cells although the doses needed for significant inductions were much higher than those by a known genotoxic agent, N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). The level of transformation induced by DDVP in RTE cells was about 1/5 that induced by MNNG at a dose of similar cytotoxicity. The slope of the regression line for induced transformation is 1.27. DDVP probably induces the genotoxic effect in RTE cells by a one-hit mechanism.
Lin
S Y
SY
Institute of Zoology, Academia Sinica, Taipei, Republic of China.
Lee
T C
TC
Cheng
C S
CS
Wang
T C
TC
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002471:Cell Transformation, Neoplastic / Q000139:chemically induced*; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D004006:Dichlorvos / Q000494:pharmacology*; D004848:Epithelium / Q000187:drug effects; D008297:Male; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology; D009152:Mutagenicity Tests; D051381:Rats; D011919:Rats, Inbred Strains; D012854:Sister Chromatid Exchange / Q000187:drug effects*; D014132:Trachea
D008769:Methylnitronitrosoguanidine; D004006:Dichlorvos