Comparison between mouse kidneys of pre- and postnatal ages maturing in vivo and in serum-free organ culture.
1. To evaluate the influence of age, DNA synthesis and brush border hydrolase activities were determined in mouse kidneys maturing in vivo and in serum-free organ culture. 2. DNA synthesis decreased with advancing age. 3. The protein content and leucylnaphthylamidase, maltase, trehalase, alkaline phosphatase and gamma-glutamyltransferase activities increased with aging. 4. The differences due to age were reproduced in kidneys maturing in culture. 5. These results show that age has a significant effect on the parameters determined, but apparently has no influence on the viability of the kidney explants in culture.
Bertrand
L
L
Département d'Anatomie et de biologie cellulaire, Faculté de Médecine, Université de Sherbrooke, Québec, Canada.
Brière
N
N
Ferrari
J
J
D000375:Aging / Q000378:metabolism; D000818:Animals; D003470:Culture Media; D004247:DNA / Q000096:biosynthesis; D004798:Enzymes / Q000378:metabolism; D007668:Kidney / Q000196:embryology / Q000254:growth & development* / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D008871:Microvilli / Q000201:enzymology; D009924:Organ Culture Techniques; D011506:Proteins / Q000378:metabolism
D003470:Culture Media; D004798:Enzymes; D011506:Proteins; D004247:DNA