Phagocytosis of polystyrene latex beads by rat brain microglia cell cultures is increased by treatment with gangliosides.
Cultures of microglial cells were prepared from primary rat brain cultures of astrocytes. Microglia appeared under two main forms: ameboid and ramified. Only the former were capable of ingesting opsonized latex particles of 1 and 6 micron; while the latter, which appeared later in culture, were not capable of phagocytosis. When gangliosides (10(-6) M), which are known for their neuronotrophic capacity in vitro, were added to the growth medium (for 20 min) they stimulated the phagocytosis of ameboid microglia 2-fold.
Bocchini
V
V
Istituto di Biologia Cellulare, Universita di Perugia, Italia.
Artault
J C
JC
Rebel
G
G
Dreyfus
H
H
Massarelli
R
R
D000818:Animals; D001921:Brain / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D005732:Gangliosides / Q000494:pharmacology*; D008854:Microscopy, Electron; D009457:Neuroglia / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D010587:Phagocytosis / Q000187:drug effects*; D051381:Rats
D005732:Gangliosides