A phosphate-repressible acid phosphatase gene from Aspergillus niger: its cloning, sequencing and transcriptional analysis.
The cloning and sequencing of an Aspergillus niger gene encoding a secreted form of phosphate-repressible acid phosphatase by complementation of a pacA (phosphate-repressible acid phosphatase) mutant of Aspergillus nidulans is described. The gene contains two introns, 201 and 265 nt in length, and codes for a 1.6-kb transcript. Both phosphate concentration and pH of the growth medium affect the level of expression of the gene in A. niger. Similar regulation is observed in A. nidulans transformants. A putative signal peptide, resembling known signal sequences of yeast, is identified.
MacRae
W D
WD
Allelix Inc., Mississauga, Ont., Canada.
Buxton
F P
FP
Sibley
S
S
Garven
S
S
Gwynne
D I
DI
Davies
R W
RW
Arst
H N
HN
D000135:Acid Phosphatase / Q000096:biosynthesis / Q000235:genetics*; D000595:Amino Acid Sequence; D001234:Aspergillus niger / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004274:DNA, Recombinant; D004794:Enzyme Repression; D005800:Genes, Fungal; D006863:Hydrogen-Ion Concentration; D007438:Introns; D008969:Molecular Sequence Data; D009154:Mutation; D010710:Phosphates / Q000494:pharmacology; D015183:Restriction Mapping; D014158:Transcription, Genetic; D014170:Transformation, Genetic
D004274:DNA, Recombinant; D010710:Phosphates; D000135:Acid Phosphatase