title: Evolution of influenza polymerase: nucleotide sequence of the PB2 gene of A/Chile/1/83 (H1 N1).
abstract: The complete nucleotide sequence of the PB2 gene of influenza virus A/Chile/1/83 (H1 N1) is presented. Sequence comparison between A/Chile PB2 protein and the known PB2 sequences of the influenza strains A/WSN/33 (H1 N1), A/PR/8/34 (H1 N1), A/NT/60/68 (H3 N2), A/Kiev/59/79 (H1 N1), A/FPV/Rostock/34 (H7 N1), and B/Ann Arbor/1/66 indicates extensive amino acid homology for the influenza A virus PB2 proteins. Small clusters of basic amino acids are conserved in all PB2 proteins including the influenza B PB2 protein which has only 39% sequence homology overall to the PB2 polypeptides of type A influenza viruses. The evolutionary rate of 5.7 x 10(-3) nucleotide substitutions per site per year and 0.25% amino acid changes per year between the A/Chile/1/83 and A/NT/60/68 PB2 appears to be higher than that calculated earlier for A/NT, A/PR/8 and A/WSN. An unusually high degree of sequence change between A/Chile/1/83 and A/Kiev/59/79 PB2 polymerase was revealed and this is discussed in terms of its probable origin.
authors.affiliation: Department of Molecular Biology, Central Institute of Hygiene, Microbiology, Berlin, German Democratic Republic.
authors.forename: E
authors.initials: E
authors.lastname: Schreier
authors.forename: D R
authors.initials: DR
authors.lastname: Petzold
authors.forename: S
authors.initials: S
authors.lastname: Michel
authors.forename: S
authors.initials: S
authors.lastname: Dittmann
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D005075:Biological Evolution; D005814:Genes, Viral; D009980:Influenza A virus / Q000235:genetics*; D008969:Molecular Sequence Data; D012316:RNA Nucleotidyltransferases / Q000235:genetics*; D012333:RNA, Messenger; D012367:RNA, Viral; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
chemical_list: D012333:RNA, Messenger; D012367:RNA, Viral; D012316:RNA Nucleotidyltransferases; D012324:RNA-Dependent RNA Polymerase