Crystallization of an anti-2,2,6,6-tetramethyl-1-piperidinyloxy-dinitrophenyl monoclonal antibody Fab fragment with and without bound hapten.
The Fab fragment of a monoclonal antibody, AN02, specific for a 2,2,6,6-tetramethyl-1-piperidinyloxy-dinitrophenyl hapten was crystallized both with and without bound hapten. Both crystals formed in phosphate-buffered saline (150 mM-NaCl, 10 mM-Na2PO4, 0.02% (w/v) NaN3 (pH 7.4) at 4 degrees C and diffracted beyond 2.2 A resolution (1A = 0.1 nm). Fab with bound hapten crystallizes in space group P6(1)22 or P6(5)22, with cell dimensions a = b = 73.23 A, c = 373.8 A, alpha = beta = 90 degrees and gamma = 120 degrees. Unoccupied Fab also crystallizes in space group P6(1)22 or P6(5)22 with cell dimensions a = b = 73 A, c = 377 A, alpha = beta = 90 degrees and gamma = 120 degrees.
Leahy
D J
DJ
Stauffer Laboratory of Physical Chemistry, Stanford University, CA 94305.
Hynes
T R
TR
McConnell
H M
HM
Fox
R O
RO
D000911:Antibodies, Monoclonal; D003460:Crystallization; D003497:Cyclic N-Oxides / Q000276:immunology*; D006241:Haptens; D007140:Immunoglobulin Fab Fragments; D014961:X-Ray Diffraction
C058178:2,2,6,6-tetramethyl-1-piperdinyloxy-dinitrophenyl; D000911:Antibodies, Monoclonal; D003497:Cyclic N-Oxides; D006241:Haptens; D007140:Immunoglobulin Fab Fragments