title: Nuclear scaffold attachment sites in the human globin gene complexes.
abstract: In an analysis of a 90-kb region around the human beta-globin gene complex we have identified at least eight sites of attachment to the nuclear scaffold (SARs). While these have many potential functions, there appears to be a particular association with sequences important in the regulation of the complex. Two SARs are close to the known enhancer-like elements of the beta-globin gene. SARs flanking the complex co-habit with the boundaries of the putative beta-like globin gene regulatory domain. In contrast, we have detected no SARs within a 140-kb region of the human alpha-globin gene complex. If SARs play a role in the regulation of gene expression then this structural difference would imply a difference in the regulation of the two complexes.
authors.affiliation: Nuffield Department of Clinical Medicine, John Radcliffe Hospital, Headington, Oxford, UK.
authors.forename: A P
authors.initials: AP
authors.lastname: Jarman
authors.forename: D R
authors.initials: DR
authors.lastname: Higgs
mesh_terms: D001665:Binding Sites; D015139:Blotting, Southern; D002467:Cell Nucleus / Q000378:metabolism*; D004247:DNA / Q000032:analysis* / Q000235:genetics; D005786:Gene Expression Regulation; D005914:Globins / Q000235:genetics*; D006801:Humans; D005810:Multigene Family; D009693:Nucleic Acid Hybridization; D015183:Restriction Mapping
chemical_list: D005914:Globins; D004247:DNA