[Proliferation of cultured cells with genetically induced resistance to ethidium bromide on synthetic nutrient media without serum].
Lebr 625 and Lebr 350 cells, resistant to ethidium bromide in concentrations 25 and 50 mkg/ml, are able to grow continuously in serum- and protein-free media. Under the same conditions the parental L929 cells are not able to. Two cell lines (625 sf and 350 sf) were established capable of growing in serum- and protein free media. It is found that ethidium bromide is toxic for resistant cells grown the in serum-free medium. The addition of serum lowers the toxic action of ethidium bromide. A continuous growth of resistant cells in serum-free medium (under nonselective conditions) leads to a decreased level of resistance, which may nevertheless persist for a long period of cultivation (over 2.5 years).
Sal'nikov
K V
KV
Pashinin
Iu V
IuV
Ignatova
T N
TN
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002461:Cell Line, Transformed; D003470:Culture Media / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004351:Drug Resistance / Q000235:genetics; D004996:Ethidium / Q000037:antagonists & inhibitors*; D007739:L Cells / Q000166:cytology* / Q000187:drug effects; D051379:Mice; D012641:Selection, Genetic; D013997:Time Factors
D003470:Culture Media; D004996:Ethidium
Proliferatsiia kul'tiviruemykh kletok s geneticheski obuslovlennoĭ ustoĭchivbost'iu k bromistomu étidiiu na sinteticheskikh pitatel'nykh sredakh bez syvorotki.