Constitutive attachment of murine erythroleukemia cell histone-depleted DNA loops to nuclear scaffolding is found in the beta-major but not the alpha 1-globin gene.
We have identified a region of the beta-globin gene that is attached constitutively to histone-depleted murine erythroleukemia cell nuclei. This region spans 800 bp and is located at -300 to -1100 bp upstream from the site of transcriptional initiation. Attachment is not altered by transcriptional activation of the beta-globin gene during induction to terminal differentiation, and the same region of the beta-globin gene is attached to histone-depleted myeloma cell nuclei (NS-1). The attached region contains an A/T-rich section, in addition to a sequence closely related to the Drosophila topoisomerase II consensus cleavage sequence. No comparable site of attachment of the alpha 1-globin gene was detected when a region spanning 1.5 kb 5' to 0.5 kb 3' of the region of transcription was studied.
Greenstein
R J
RJ
Veterans Administration Medical Center, Bronx, NY 10468.
D000818:Animals; D002460:Cell Line; D002843:Chromatin / Q000648:ultrastructure; D004273:DNA, Neoplasm / Q000648:ultrastructure*; D004330:Drosophila / Q000235:genetics; D005914:Globins / Q000032:analysis / Q000235:genetics*; D004915:Leukemia, Erythroblastic, Acute / Q000235:genetics*; D051379:Mice; D012091:Repetitive Sequences, Nucleic Acid; D014158:Transcription, Genetic
D002843:Chromatin; D004273:DNA, Neoplasm; D005914:Globins