title: Simple assay for sialyltransferase activity with a new fluorogenic substrate.
abstract: A new fluorogenic acceptor for sialyltransferase, 2-[(2-pyridyl)amino]ethyl O-beta-D-galactopyranosyl-(1----4)-beta-D-glucopyranoside, was prepared from lactose as a starting material. Sialyltransferase activity was assayed by incubation of the enzyme with the acceptor and CMP-N-acetylneuraminic acid, separation of the fluorogenic sialylated product from the enzymatic reaction mixture by HPLC, and measurement of the product. Compared to assays so far reported that use radioactive substrates, this assay is simple and rapid. This method was used to assay sialyltransferase activity in human serum.
authors.affiliation: Department of Chemistry, Osaka University College of Science.
authors.forename: T
authors.initials: T
authors.lastname: Sato
authors.forename: K
authors.initials: K
authors.lastname: Omichi
authors.forename: T
authors.initials: T
authors.lastname: Ikenaka
mesh_terms: D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007202:Indicators and Reagents; D012799:Sialyltransferases / Q000097:blood*; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D007202:Indicators and Reagents; D012799:Sialyltransferases