Effect of platelet-activating factor on leukocyte adhesion to microvascular endothelium. Time course and dose-response relationships.
The hamster cheek pouch microcirculation was used to investigate the effects of platelet-activating factor (PAF) on leukocyte adhesion to microvascular walls by means of intravital microscopy. PAF was applied topically at concentrations ranging from 10(-11) to 10(-5) M. An inverse relationship between PAF concentration and number of adhering white cells per 100-microns length was found in venules ranging in diameter from 10 to 60 microns (grouped into 10-microns intervals). Importantly, the PAF-induced adhesion of leukocytes lasted for the 3-h experimental period. We postulate that induction of leukocyte adhesion to venular endothelium is an important role of PAF in inflammatory processes.
Dillon
P K
PK
Department of Physiology, UMDNJ-New Jersey Medical School, Newark 07103-2757.
Fitzpatrick
M F
MF
Ritter
A B
AB
Durán
W N
WN
D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D006224:Cricetinae; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000187:drug effects*; D007962:Leukocytes / Q000187:drug effects*; D008297:Male; D008647:Mesocricetus; D010972:Platelet Activating Factor / Q000494:pharmacology*; D013997:Time Factors
D010972:Platelet Activating Factor