Construction of three plasmids, each containing two or three different human globin gene fragments.
Three plasmids (labeled pUC-2 gamma-beta, pUC-2 beta-delta, and pL-alpha-zeta), each containing two or three different human globin gene fragments (beta IVS-II, inter-gamma, and gamma IVS-II in pUC-2 gamma-beta; 5'-beta, 3'-beta, and delta IVS-II in pUC-2 beta-delta; alpha and zeta in pL-alpha-zeta) have been constructed. The gene fragments could be isolated simultaneously from Eco RI/Bam HI double digests of each of the first two plasmids or from a Pst I digest of the third, using electrophoresis in an 1.0% agarose gel. This facilitates the large-scale isolation of gene fragments for use as probes in studies such as the analysis of polymorphisms or the molecular basis of some hemoglobinopathies.
Liu
J Z
JZ
Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Beijing.
Huisman
T H
TH
D004247:DNA / Q000302:isolation & purification; D004274:DNA, Recombinant / Q000032:analysis; D005820:Genetic Testing; D005822:Genetic Vectors; D005914:Globins / Q000235:genetics*; D006801:Humans; D010957:Plasmids; D014170:Transformation, Genetic
D004274:DNA, Recombinant; D005914:Globins; D004247:DNA