Predicting the fertilising ability of avian semen: the development of an objective colourimetric method for assessing the metabolic activity of fowl spermatozoa.
1. A simple, objective colourimetric technique for estimating the capacity of fowl spermatozoa to reduce a tetrazolium dye is described. 2. The test is shown to give a quantitative measurement of the metabolic activity of spermatozoa, as judged by their rate of oxygen utilisation, and thus of the 'quality' of semen in poultry.
Chaudhuri
D
D
AFRC Institute of Animal Physiology and Genetics Research, Edinburgh Research Station, Roslin, Midlothian, Scotland.
Wishart
G J
GJ
D000818:Animals; D002645:Chickens / Q000502:physiology*; D003124:Colorimetry / Q000379:methods; D005306:Fertilization; D008297:Male; D010101:Oxygen Consumption; D012661:Semen / Q000032:analysis*; D013094:Spermatozoa / Q000378:metabolism*; D013778:Tetrazolium Salts / Q000378:metabolism
D013778:Tetrazolium Salts; C013574:iodonitrotetrazolium