The effect of calibration on the between-laboratory variation of serum fructosamine.
Human pooled serum from diabetic and non-diabetic subjects, and calibrators containing glycated protein or 1-deoxy-1-morpholinofructose (DMF) in a variety of matrices, were distributed to 10 laboratories. When they used their own assay conditions and calibrators, the inter-laboratory variation was unacceptably high. However, when the pool from diabetic patients was reassayed using a calibrator with an assigned value prepared from freeze-dried human serum, and containing no DMF, inter-laboratory variation was reduced significantly. Inter-laboratory agreement for the pool from non-diabetic subjects remained poor despite recalibration. Recalibration using either serum or albumin based solutions of DMF as calibrator failed to effect any significant reductions in inter-laboratory variation. Secondary calibrators based on a protein matrix with no added DMF are recommended for routine use.
Hill
R P
RP
Department of Clinical Chemistry, King's Mill Hospital, Sutton-in-Ashfield, Nottinghamshire, UK.
D002138:Calibration; D019270:Fructosamine; D005632:Fructose / Q000031:analogs & derivatives; D006595:Hexosamines / Q000097:blood*; D006801:Humans; D007753:Laboratories / Q000592:standards*; D009025:Morpholines; D012709:Serum Albumin; D014894:Weights and Measures
D006595:Hexosamines; D009025:Morpholines; D012709:Serum Albumin; D005632:Fructose; D019270:Fructosamine; C044913:1-deoxy-1-morpholinofructose