Improved procedure for the construction of neoglycolipids having antigenic and lectin-binding activities, from reducing oligosaccharides.
Conditions have been established for the rapid and efficient conjugation of reducing oligosaccharides (di- to deca-saccharides) to dipalmitoyl phosphatidylethanolamine. The resulting neoglycolipids derived from several naturally occurring oligosaccharides and a series of N-linked high-mannose-type oligosaccharides released by hydrazinolysis from RNAase B showed specific and potent reactivities, as appropriate, with monoclonal antibodies to blood group Lewis(b), blood group A or a stage-specific embryonic (SSEA-1) antigen, or the lectin concanavalin A.
Stoll
M S
MS
Section of Glycoconjugate Research, MRC Clinical Research Centre, Harrow, Middx., U.K.
Mizuochi
T
T
Childs
R A
RA
Feizi
T
T
D000586:Amination; D000918:Antibody Specificity; D003208:Concanavalin A; D006017:Glycolipids / Q000138:chemical synthesis* / Q000276:immunology; D006801:Humans; D015335:Molecular Probes / Q000138:chemical synthesis; D009844:Oligosaccharides; D010714:Phosphatidylethanolamines
D006017:Glycolipids; D015335:Molecular Probes; D009844:Oligosaccharides; D010714:Phosphatidylethanolamines; D003208:Concanavalin A; C007510:1,2-dipalmitoyl-3-phosphatidylethanolamine