Protein serotyping of Streptococcus pneumoniae based on reactivity to six monoclonal antibodies.
Six monoclonal antibodies to proteins of Streptococcus pneumoniae were tested in a dot blot assay for reactivity with 499 clinical isolates of pneumococci. Forty-four percent of the isolates reacted with at least one of the antibodies. Nineteen patterns of reactivity were identified and each designated as a provisional protein serotype. Protein serotyping identified pneumococcal strains independently of their capsular type and made it possible to differentiate strains within most capsular types. A protein serotyping system provides a new dimension to the phenotypic identification of S. pneumoniae and may eventually provide a basis for assessing the population structure of these organisms.
Waltman
W D
WD
Department of Microbiology, University of Alabama, Birmingham 35294.
McDaniel
L S
LS
Andersson
B
B
Bland
L
L
Gray
B M
BM
Eden
C S
CS
Briles
D E
DE
D000818:Animals; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000145:classification; D051379:Mice; D012703:Serotyping / Q000379:methods; D013296:Streptococcus pneumoniae / Q000145:classification*
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface