[Clinical usefulness of a trypsin radioimmunoassay kit].
From the clinical use of RIA-gnost trypsin kit, the following results were obtained. 1. Standard curve showed a steep and good curve was shown. 2. Incubation: The condition for the first incubation was set at the room temperature for 10-24 hours and that for the second incubation at the room temperature for 3-5 hours. With these settings, satisfactory results were obtained. 3. Reproducibility and recovery: The C.V. of the reproducibility and the recovery were considered superior, and the values were below 10% and +/- 3%, respectively. 4. Correlation between trypsin and serum elestase-1: An excellent positive correlation (coefficient of correlation r = 0.889) was shown. 5. Serum trypsin concentration of normal and pancreatic diseases: The normal range was from 100 to 500 ng/ml. Acute pancreatitis rose obviously. Diabetes mellitus and chronic pancreatitis was below 500 ng/ml and the pancreatic cancer showed a tendency to scatter in the range of 50-1,250 ng/ml. The above results indicated that serum trypsin can be easily measured with high precision by using this method. Thus the method is considered useful for the diagnosis of pancreatic diseases.
Kanamori
I
I
Department of Radionuclear Medicine, Ohgaki Municipal Hospital, Japan.
Nakano
S
S
Hurukawa
M
M
Okumura
Y
Y
Higuchi
C
C
Yanase
M
M
Onogi
M
M
D006801:Humans; D010182:Pancreatic Diseases / Q000175:diagnosis; D011863:Radioimmunoassay; D011933:Reagent Kits, Diagnostic; D014357:Trypsin / Q000097:blood*
D011933:Reagent Kits, Diagnostic; D014357:Trypsin