title: A 13 kDa fragment is responsible for the hydrophobic aggregation of brain G4 acetylcholinesterase.
abstract: Proteinase K treatment of the bovine brain acetylcholinesterase (AChE) releases a hydrophobic fragment of 13 kDa, which is entirely responsible for the aggregation of the G4 AChE in the absence of detergent. This observation provides evidence that the 13 kDa fragment, which comes from a previously identified 20 kDa subunit, is directly involved in the attachment of the G4 AChE to brain membranes. A model for the organization of the different sub-domains of the hydrophobic anchor of the G4 AChE is presented.
authors.affiliation: Department of Cell Biology, Faculty of Biological Sciences and Medicine, Catholic University of Chile, Santiago.
authors.forename: M E
authors.initials: ME
authors.lastname: Fuentes
authors.forename: T L
authors.initials: TL
authors.lastname: Rosenberry
authors.forename: N C
authors.initials: NC
authors.lastname: Inestrosa
mesh_terms: D000110:Acetylcholinesterase / Q000502:physiology*; D000818:Animals; D002417:Cattle; D002421:Caudate Nucleus / Q000201:enzymology*; D019286:Endopeptidase K; D008970:Molecular Weight; D010446:Peptide Fragments / Q000502:physiology*; D012697:Serine Endopeptidases
chemical_list: D010446:Peptide Fragments; D000110:Acetylcholinesterase; D012697:Serine Endopeptidases; D019286:Endopeptidase K