Enzymatic replacement of RNA sequences in vitro; use of FPLC for rapid and efficient preparation of tRNA variants.
A chromatographic procedure using columns of Superose-12 (gel filtration) and PBA-30 (phenylboronate agarose) in tandem has been developed. This procedure is very useful for rapidly separating and efficiently recovering each of the components in the reaction mixture of joining reaction with RNA ligase, and thereby is also beneficial to rapid preparation of tRNA variants in a high yield.
Nishikawa
K
K
Department of Molecular Biology, School of Science, Nagoya University, Japan.
D002850:Chromatography, Gel / Q000379:methods; D014644:Genetic Variation; D007202:Indicators and Reagents; D011130:RNA Ligase (ATP); D012343:RNA, Transfer / Q000235:genetics / Q000302:isolation & purification*; D012997:Solvents
D007202:Indicators and Reagents; D012997:Solvents; D012343:RNA, Transfer; D011130:RNA Ligase (ATP)