Effect of radiofrequency radiation on mRNA expression in cultured rodent cells.
Four rodent cell lines were exposed to 2450 MHz microwave radiation at a Specific Absorption Rate (SAR) of 103.5 +/- 4.2 W/kg for varying lengths of time at 37 degrees, 40 degrees, 42 degrees and 45 degrees C. mRNA was extracted from microwave-exposed and sham-exposed cells and dot blotted or Northern blotted to nitrocellulose. Radioisotope labelled DNA probes of oncogenes, heat shock protein or long terminal repeat sequences were hybridized to the mRNA, and the resulting autoradiographs analyzed for differences in levels of mRNA expression between exposed and nonexposed samples. With the cell lines and probes used in this study no significant differences in mRNA expression were observed after microwave exposure.
Parker
J E
JE
Department of Microbiology, University of Texas Health Science Center, San Antonio.
Kiel
J L
JL
Winters
W D
WD
D000818:Animals; D015152:Blotting, Northern; D002460:Cell Line; D006224:Cricetinae; D015342:DNA Probes; D051379:Mice; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000235:genetics / Q000528:radiation effects*; D011846:Radio Waves; D014158:Transcription, Genetic / Q000528:radiation effects*
D015342:DNA Probes; D012333:RNA, Messenger