title: Increase of dentin phosphophoryn with dentin formation.
abstract: Dentin phosphophoryn was quantified on bovine and rabbit dentin at three developmental stages. Phosphophoryn was extracted from teeth with 0.6M HCl, and quantified as optical density on DEAE-cellulose chromatogram or as phosphoserine content. Bovine phosphophoryn showed progressive increase with formation of dentin. Matrix-associated phosphophoryn was also quantified as phosphoserine content in insoluble dentin residue which was extracted with 6 M urea after decalcification. This fraction increased with formation of dentin both in bovine and rabbit dentin. Phosphophoryn is thought to be related to the later stage of dentin formation.
authors.affiliation: Department of Biochemistry, School of Dentistry, Hokkaido University, Sapporo, Japan.
authors.forename: R
authors.initials: R
authors.lastname: Fujisawa
authors.forename: Y
authors.initials: Y
authors.lastname: Kuboki
mesh_terms: D000818:Animals; D002417:Cattle; D002848:Chromatography, DEAE-Cellulose; D003810:Dentinogenesis; D004591:Electrophoresis, Polyacrylamide Gel; D007180:Incisor / Q000032:analysis; D010750:Phosphoproteins / Q000032:analysis*; D011817:Rabbits; D012967:Sodium Dodecyl Sulfate
chemical_list: D010750:Phosphoproteins; C017630:phosphophoryn; D012967:Sodium Dodecyl Sulfate