Effect of ischemia and reperfusion of the myocardium on in vitro beta-oxidation of fatty acids.
The in vivo oxidation of perfused [14C]-labeled fatty acids has been shown to decrease dramatically in hypoxic hearts. This study addresses the influence of ischemia and reperfusion on the enzymic activities of beta-oxidation of fatty acids in mitochondria and of peroxisomal origin. The rate of beta-oxidation of fatty acids in the isolated mitochondria from myocardium of swine fed control diet declined about 20% by the ischemic insult induced by hypothermic cardioplegic arrest. Upon reperfusion, the rate of mitochondrial beta-oxidation returned to a normal level. In clofibrate-fed animals, the rate of mitochondrial beta-oxidation did not vary significantly between control, ischemic, and perfused tissues. Furthermore, neither in control nor in clofibrate-fed animals did the rates of peroxisomal beta-oxidation of fatty acids vary significantly in the ischemic or reperfused tissues as compared to that of preischemic controls. These results suggest that ischemia does not contribute to any loss of enzymic activity in beta-oxidation of fatty acid cycles either in mitochondria or peroxisomes. Furthermore, the feeding of 0.5% (w/w) clofibrate to pigs increased the rate of mitochondrial beta-oxidation of fatty acids only by 50% while that of peroxisomes increased threefold. A similar threefold increase in catalase activity was also produced by clofibrate feeding. These results suggest that the heart plays a role in the hypolipidemic action of clofibrate.
Prasad
M R
MR
University of Connecticut Health Center, Farmington.
Clement
R
R
Jones
R
R
Engelman
R M
RM
Otani
H
H
Das
D K
DK
D000818:Animals; D002374:Catalase / Q000378:metabolism; D002994:Clofibrate / Q000494:pharmacology; D003327:Coronary Disease / Q000378:metabolism*; D003402:Creatine Kinase / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism*; D006324:Heart Arrest, Induced; D008830:Microbodies / Q000378:metabolism; D008929:Mitochondria, Heart / Q000378:metabolism; D015425:Myocardial Reperfusion; D010084:Oxidation-Reduction; D010725:Phosphocreatine / Q000378:metabolism; D013552:Swine
D005227:Fatty Acids; D010725:Phosphocreatine; D002374:Catalase; D003402:Creatine Kinase; D002994:Clofibrate