title: Expression of human angiogenin in cultured baby hamster kidney cells.
abstract: Baby hamster kidney cells were transformed with DNA sequences derived from the gene for human angiogenin. Expression was under the transcriptional control of the inducible mouse metallothionein 1 promoter. Recombinant angiogenin was purified and shown to be chemically, biologically, and enzymatically indistinguishable from the natural product. The large-scale production of recombinant angiogenin achieved should facilitate detailed studies into the structure-function relationships of this potent angiogenic molecule.
authors.affiliation: Center for Biochemical and Biophysical Sciences and Medicine, Harvard Medical School, Boston, Massachusetts 02115.
authors.forename: K
authors.initials: K
authors.lastname: Kurachi
authors.forename: S M
authors.initials: SM
authors.lastname: Rybak
authors.forename: J W
authors.initials: JW
authors.lastname: Fett
authors.forename: R
authors.initials: R
authors.lastname: Shapiro
authors.forename: D J
authors.initials: DJ
authors.lastname: Strydom
authors.forename: K A
authors.initials: KA
authors.lastname: Olson
authors.forename: J F
authors.initials: JF
authors.lastname: Riordan
authors.forename: E W
authors.initials: EW
authors.lastname: Davie
authors.forename: B L
authors.initials: BL
authors.lastname: Vallee
mesh_terms: D043925:Angiogenesis Inducing Agents / Q000235:genetics*; D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D005796:Genes; D005822:Genetic Vectors; D006133:Growth Substances / Q000235:genetics*; D006801:Humans; D007668:Kidney; D009363:Neoplasm Proteins / Q000235:genetics* / Q000302:isolation & purification; D010446:Peptide Fragments / Q000032:analysis; D010957:Plasmids; D011994:Recombinant Proteins / Q000302:isolation & purification; D012259:Ribonuclease, Pancreatic; D014158:Transcription, Genetic; D014162:Transfection
chemical_list: D043925:Angiogenesis Inducing Agents; D006133:Growth Substances; D009363:Neoplasm Proteins; D010446:Peptide Fragments; D011994:Recombinant Proteins; C046366:angiogenin; D012259:Ribonuclease, Pancreatic