Enzymatic amplification and characterization of large DNA fragments from genomic DNA.
Conditions for DNA amplification in vitro using modified T7 DNA polymerase have been devised to obtain 2000-bp DNA fragments of the HGPRT gene directly from human genomic DNA. The DNA obtained from a 1.2 x 10(5)-fold amplification has been used for direct sequencing.
Keohavong
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MIT Center for Environmental Health Sciences, Massachusetts Institute of Technology, Cambridge 02139.
Wang
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Cha
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Thilly
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D001483:Base Sequence; D004261:DNA Replication; D004259:DNA-Directed DNA Polymerase; D005784:Gene Amplification; D006801:Humans; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics; D009838:Oligodeoxyribonucleotides
D009838:Oligodeoxyribonucleotides; D007041:Hypoxanthine Phosphoribosyltransferase; D004259:DNA-Directed DNA Polymerase