HPLC analysis of the phenylisothiocyanate (PITC) derivatives of taurine from physiologic samples.
Taurine is the major free intracellular amino acid. It has become the focus of study by many as a conjugator of bile and as a neurotransmitter and intracellular messenger. In this report we document a technique for measuring taurine in physiologic samples which is rapid, reproducible, and accurate. Any physiologic sample is first derivatized with phenylisothiocyanate (PITC) and separated by reverse phase HPLC, and then taurine is detected by UV at 254 nm. The advantages of this technique for the measurement of taurine are accuracy, small sample size, and reproducibility, and with an automated system many samples can be analyzed.
Lippincott
S E
SE
Department of Pediatrics, University of Wisconsin Hospital and Clinics, Madison 53792.
Friedman
A L
AL
Siegel
F L
FL
Pityer
R M
RM
Chesney
R W
RW
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D017879:Isothiocyanates; D051381:Rats; D013654:Taurine / Q000032:analysis*; D013861:Thiocyanates
D000596:Amino Acids; D017879:Isothiocyanates; D013861:Thiocyanates; C005441:phenylisothiocyanate; D013654:Taurine