A technique for collecting saliva from the cattle-tick Boophilus microplus (Canestrini, 1887) using chemical stimulation. Environmental and temporal influences on secretion yield.
1. Injection of a pilocarpine solution into the hemocoele of female B. microplus through the respiratory spiracle induced the flow of limpid saliva, collected from the mouth parts with a capillary tube. 2. The time interval between the detachment of the tick from its host and chemical stimulation influenced the volume of saliva secreted; secretion was greater during the first 2 h after detachment. 3. There is a positive correlation between salivary yield and both environmental temperature and relative humidity.
Bechara
G H
GH
Departamento de Patologia Veterinária, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista, Brasil.
Szabó
M P
MP
Machado
R Z
RZ
Rocha
U F
UF
D000818:Animals; D002417:Cattle; D008685:Meteorological Concepts; D010862:Pilocarpine / Q000494:pharmacology*; D012463:Saliva / Q000378:metabolism*; D013048:Specimen Handling / Q000379:methods*; D013268:Stimulation, Chemical; D013987:Ticks / Q000502:physiology*
D010862:Pilocarpine