title: The endogenous muscarinic acetylcholine receptor in Xenopus oocytes is of the M3 subtype.
abstract: The muscarinic acetylcholine receptor of the Xenopus oocyte was characterized electrophysiologically. Iontophoretic responses to acetylcholine were inhibited by the muscarinic antagonists 4-DAMP, pirenzepine and AF-DX 116 at respective IC50 values of 7 nM, 15 microM and 2 microM. This antagonist sensitivity order indicates that the receptor is of the M3 muscarinic subtype.
authors.affiliation: CNS Pharmacology R&D Laboratory, Organon Int. B.V., Oss, The Netherlands.
authors.forename: L A
authors.initials: LA
authors.lastname: Van Wezenbeek
authors.forename: J A
authors.initials: JA
authors.lastname: Tonnaer
authors.forename: G S
authors.initials: GS
authors.lastname: Ruigt
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D005260:Female; D066298:In Vitro Techniques; D008564:Membrane Potentials / Q000187:drug effects; D009865:Oocytes / Q000378:metabolism*; D010880:Piperidines / Q000494:pharmacology; D010890:Pirenzepine / Q000031:analogs & derivatives / Q000494:pharmacology; D011950:Receptors, Cholinergic / Q000378:metabolism*; D014982:Xenopus laevis
chemical_list: D010880:Piperidines; D011950:Receptors, Cholinergic; D010890:Pirenzepine; C042375:4-diphenylacetoxy-1,1-dimethylpiperidinium; D000109:Acetylcholine; C048593:otenzepad