DNA-polymerase activity detected in situ. Relationship between incorporation of 3H-deoxytriphosphates and 3H-actinomycin D binding during the cell cycle in antheridial filaments of Chara vulgaris L. Effect of auxin and cytokinins.
DNA-polymerase activity during the cell cycle (S + G2 + M + C type) in antheridial filaments cells of Chara vulgaris was studied using the autoradiographic method. Incorporation of 3H-deoxytriphosphates (3H-dTPs) during the whole of interphase indicates, that the cell cycle is not accompanied by distinct changes in enzyme activity. Incorporation of 3H-dTPs was also observed in spermatids and in early stages of spermatogenesis. Intensity of 3H-dTPs incorporation during interphase and spermatogenesis is similar to the intensity of 3H-actinomycin D (3H-AMD) binding. Auxin (IAA) and kinetin stimulate both 3H-AMD binding and 3H-dTPs incorporation; benzyladenine does not affect any of these processes. The in situ autoradiographic method of detecting DNA-polymerase activity reveals availability of DNA template for the enzyme rather than DNA polymerase activity itself.
Godlewski
M
M
Department of Plant Cytology and Cytochemistry, University of Lodz, Poland.
D001345:Autoradiography; D002453:Cell Cycle; D003583:Cytokinins / Q000494:pharmacology; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D003609:Dactinomycin / Q000378:metabolism; D003854:Deoxyribonucleotides / Q000378:metabolism; D007210:Indoleacetic Acids / Q000494:pharmacology; D007701:Kinetin / Q000494:pharmacology; D059828:Plant Cells; D010944:Plants / Q000187:drug effects / Q000378:metabolism*
D003583:Cytokinins; D003854:Deoxyribonucleotides; D007210:Indoleacetic Acids; D003609:Dactinomycin; D004259:DNA-Directed DNA Polymerase; D007701:Kinetin