title: The effect of 3-methylindole on the quantity and functional quality of lung surfactant.
abstract: Acute bovine pulmonary edema is a naturally occurring lung disease caused by 3-methylindole (3MI), a ruminal fermentation product of tryptophan. Morphological and in vitro studies have suggested that 3MI causes abnormalities in phospholipid synthesis. The present study was designed to investigate the effect of 3MI on the quantity and functional quality of surfactant using the goat as an experimental model. Following intravenous infusion of 3MI, goats were killed at 6-, 18-, and 30-h intervals. The lungs were removed and intracellular surfactant, in the form of lamellar bodies, and extracellular surfactant from alveolar lavage were quantified. 3MI treatment did cause modest changes in the lamellar body phospholipid pools, decreasing the quantity of phosphatidylcholine and the proportion of palmitate in this fraction. The quantity of lavage phospholipids was not significantly affected. There was an increase in the protein content of the lavage, reflecting the presence of edema. The functional quality of the surfactant isolated from the lavage fraction was tested in vitro using a pulsating bubble surfactometer. 3MI infusion decreased the ability of surfactant to lower the surface tension of an air bubble at maximum radius and during compression.
authors.affiliation: Department of Nutritional Sciences, College of Biological Science, University of Guelph, Ont., Canada.
authors.forename: J B
authors.initials: JB
authors.lastname: Kirkland
authors.forename: T M
authors.initials: TM
authors.lastname: Bray
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects; D006041:Goats; D007211:Indoles / Q000494:pharmacology*; D050356:Lipid Metabolism; D008297:Male; D009929:Organ Size / Q000187:drug effects; D010168:Palmitates / Q000494:pharmacology; D010713:Phosphatidylcholines / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism; D011663:Pulmonary Surfactants / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism*; D012862:Skatole / Q000494:pharmacology*; D013500:Surface Tension
chemical_list: D007211:Indoles; D010168:Palmitates; D010713:Phosphatidylcholines; D010743:Phospholipids; D011663:Pulmonary Surfactants; D012862:Skatole