Rhodopsin-lumirhodopsin phototransition of bovine rhodopsin investigated by Fourier transform infrared difference spectroscopy.
The rhodopsin-lumirhodopsin transition has been investigated by Fourier transform infrared difference spectroscopy using isotope-labeled retinals. In the transition, two protonated carboxyl groups are involved. Another carbonyl band, located at 1725 cm-1 in rhodopsin, is shifted to 1731.5 cm-1 in lumirhodopsin. This line is tentatively assigned to a carbonyl stretching vibration of a peptide bond adjacent to the nitrogen of a proline residue. The C=N stretching vibration of rhodopsin could unequivocally be assigned to a band at 1659 cm-1. In contrast to rhodopsin and bathorhodopsin, the C=N stretching vibration of lumirhodopsin is at a low position, i.e., at 1635 cm-1, and exhibits only a downshift of 4 cm-1 upon deuteriation of the nitrogen. The C15-H rocking vibration of rhodopsin is assigned to the unusual high position of 1456 cm-1 and shifts into the normal region upon formation of lumirhodopsin. From these results, it is concluded that, whereas the environment of the Schiff base in rhodopsin, bathorhodopsin, and isorhodopsin is approximately the same, large changes occur with the formation of lumirhodopsin. From the assignment of the C10-C11 stretching vibration in bathorhodopsin and lumirhodopsin, a 10-s-cis geometry of lumirhodopsin can be excluded.
Ganter
U M
UM
Institut für Biophysik und Strahlenbiologie, Albert-Ludwig Universität Freiburg, FRG.
Gärtner
W
W
Siebert
F
F
D000818:Animals; D002417:Cattle; D003903:Deuterium; D005583:Fourier Analysis; D010777:Photochemistry; D012168:Retinal Pigments / Q000031:analogs & derivatives; D012243:Rhodopsin / Q000031:analogs & derivatives; D013055:Spectrophotometry, Infrared
D012168:Retinal Pigments; C018586:lumirhodopsin; D012243:Rhodopsin; D003903:Deuterium