Biosynthesis of epsilon-rhodomycinone from glucose by Streptomyces C5 and comparison with intermediary metabolism of other polyketide-producing streptomycetes.
The catabolism of glucose by Streptomyces C5, a producer of anthracycline antibiotics, was investigated to determine the pathways that supply precursors for anthracycline biosynthesis. Carbons for the biosynthesis of epsilon-rhodomycinone, an anthracycline aglycone, from radiolabelled glucose were derived primarily from the Embden-Meyerhof-Parnas pathway, with a minor contribution from the pentose phosphate pathway. Furthermore, the anthracycline-producing strain, Streptomyces C5, as well as Streptomyces aureofaciens and Streptomyces lividans, strains that produce nonanthracycline polyketide antibiotics, displayed enzyme activities indicative of the Embden-Meyerhof-Parnas and pentose phosphate glycolytic pathways. As determined from labelling patterns, Streptomyces C5 apparently has a complete tricarboxylic acid cycle, but does not have a glyoxylate bypass pathway.
Dekleva
M L
ML
Department of Microbiology, Ohio State University, Columbus 43210.
Strohl
W R
WR
D018943:Anthracyclines; D000903:Antibiotics, Antineoplastic / Q000096:biosynthesis*; D002952:Citric Acid Cycle; D005947:Glucose / Q000378:metabolism*; D006019:Glycolysis; D006038:Glyoxylates / Q000378:metabolism; D008316:Malonyl Coenzyme A / Q000096:biosynthesis; D010427:Pentose Phosphate Pathway; D013302:Streptomyces / Q000201:enzymology / Q000378:metabolism*
D018943:Anthracyclines; D000903:Antibiotics, Antineoplastic; D006038:Glyoxylates; C015490:rhodomycinone; D008316:Malonyl Coenzyme A; D005947:Glucose