Quantification of urinary glucose and protein with test-strips through reflectometric analysis.
Reflectometric measurement of urine test-strips, using the Urotron RL9, was employed to quantify urinary glucose and protein. Although the test-strips (Combur-9-Test) are designed for qualitative use, the reflectance intensities determined by the Urotron RL9 are applicable to quantitative analysis. Reflectance quantitation and conventional colorimetric methods were closely correlated for glucose (r = 0.953) and protein (r = 0.906). Intra-assay coefficients of variation of reflectance value were less than 5%. The negative influence of ascorbic acid on glucose determination was defined quantitatively. A significant positive interference from hemoglobin on protein determination was also demonstrated, but effectively eliminated after compensating for urine color.
Yamane
N
N
Department of Laboratory Medicine, Kumamoto University Medical School, Japan.
Sakamoto
F
F
Matsuura
F
F
D001205:Ascorbic Acid / Q000032:analysis; D001663:Bilirubin; D002138:Calibration; D003124:Colorimetry; D006029:Glycosuria / Q000652:urine*; D006454:Hemoglobins; D006801:Humans; D010783:Photometry; D011507:Proteinuria / Q000652:urine*; D015203:Reproducibility of Results
D006454:Hemoglobins; D001205:Ascorbic Acid; D001663:Bilirubin