title: The spleen colony technique. II. Errors in estimation of the fraction of CFU-s synthesizing DNA.
abstract: A refinement of the method for determining the fraction of CFU-s in the S phase is presented. The new procedures were tested by means of a model experiment in which the fraction of CFU-s 'killed' was simulated by diluting the cell suspension. Another test was to determine the fraction of the CFU-s in the S phase using duplicate samples.
authors.affiliation: Department of Pathophysiology, Faculty of Medicine, Charles University, Prague, Czechoslovakia.
authors.forename: V
authors.initials: V
authors.lastname: Znojil
authors.forename: E
authors.initials: E
authors.lastname: Necas
mesh_terms: D000818:Animals; D003114:Colony-Forming Units Assay / Q000379:methods*; D004247:DNA / Q000096:biosynthesis; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D007399:Interphase; D051379:Mice; D008815:Mice, Inbred Strains; D013154:Spleen / Q000166:cytology*; D013936:Thymidine / Q000633:toxicity
chemical_list: D004247:DNA; D013936:Thymidine