Determination of hydroxylamine traces in propionohydroxamic acid bulk drug and pharmaceutical preparations by capillary gas chromatography.
A specific, sensitive, accurate, and precise capillary gas chromatographic assay for determining trace levels of hydroxylamine, a well-known mutagen, in propionohydroxamic acid bulk drug and oral preparations is described. The analytical procedure involves derivatization in a nonaqueous medium with cyclohexanone and use of an internal standard. The derivative is then determined by capillary GC with a cool on-column injector and a nitrogen-selective detector. Effects of different matrices on the measurement were also determined. The lower limit of quantitation was 7 ppm, and the response was linear from 10 to 260 ppm. The procedure is simple and rapid enough for routine purposes.
Lombardi
F
F
Analytical Development Laboratory, Zambeletti S.p.A., Milan, Italy.
Crolla
T
T
D002214:Capsules; D055598:Chemical Phenomena; D002621:Chemistry; D002849:Chromatography, Gas; D006877:Hydroxamic Acids / Q000032:analysis*; D006898:Hydroxylamines / Q000032:analysis*; D007202:Indicators and Reagents; D012015:Reference Standards
D002214:Capsules; D006877:Hydroxamic Acids; D006898:Hydroxylamines; D007202:Indicators and Reagents; C036612:propionohydroxamic acid