title: Effect of platelet-activating factor on leukocyte adhesion to microvascular endothelium. Time course and dose-response relationships.
abstract: The hamster cheek pouch microcirculation was used to investigate the effects of platelet-activating factor (PAF) on leukocyte adhesion to microvascular walls by means of intravital microscopy. PAF was applied topically at concentrations ranging from 10(-11) to 10(-5) M. An inverse relationship between PAF concentration and number of adhering white cells per 100-microns length was found in venules ranging in diameter from 10 to 60 microns (grouped into 10-microns intervals). Importantly, the PAF-induced adhesion of leukocytes lasted for the 3-h experimental period. We postulate that induction of leukocyte adhesion to venular endothelium is an important role of PAF in inflammatory processes.
authors.affiliation: Department of Physiology, UMDNJ-New Jersey Medical School, Newark 07103-2757.
authors.forename: P K
authors.initials: PK
authors.lastname: Dillon
authors.forename: M F
authors.initials: MF
authors.lastname: Fitzpatrick
authors.forename: A B
authors.initials: AB
authors.lastname: Ritter
authors.forename: W N
authors.initials: WN
authors.lastname: Durán
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D006224:Cricetinae; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000187:drug effects*; D007962:Leukocytes / Q000187:drug effects*; D008297:Male; D008647:Mesocricetus; D010972:Platelet Activating Factor / Q000494:pharmacology*; D013997:Time Factors
chemical_list: D010972:Platelet Activating Factor