title: Cytologic changes in the nasal secretions during the immediate nasal response.
abstract: In 102 randomly selected patients with allergic rhinitis caused by immediate hypersensitivity, nasal provocation tests (NPTs) with allergens were evaluated by means of rhinomanometry, and cytologic examination of the nasal secretions (NSs) was performed. The cells in NS of 117 positive immediate nasal responses (INRs) and in 68 negative INRs, correlating with history and skin tests, and in 102 control challenges with phosphate-buffered saline were stained by modified May-Grünwald-Giemsa, toluidine blue, and Hansel's method. The positive INR was accompanied by significant changes in the count of eosinophils (increase followed by decrease) in 67% of neutrophils (decrease followed by increase) in 40%, goblet cells (increase followed by decrease) in 41%, and basophils (decrease) in 13% of the NSs. No significant changes in the count of other types of cells in the NSs were recorded during most of the cases of INR. No significant changes in the count of individual cell types in NSs were found during most cases of negative INR. During the phosphate-buffered saline control challenges, the individual cell types appeared irregularly, and no significant changes in their count were recorded in any patient. The cytologic examination of NS, evoked by allergen, appears therefore to be a valuable supplementary diagnostic parameter for nasal allergy. The repeated counting of eosinophils in NS, before and after allergen challenge, appears to be the best way to discriminate between positive and negative nasal responses, since the eosinophils demonstrated significant changes in their count during 67% of the positive and only 11% of the negative INRs.
authors.affiliation: Department of Allergology and Immunology, Institute of Medical Sciences De Klokkenberg, Breda, The Netherlands.
authors.forename: Z
authors.initials: Z
authors.lastname: Pelikan
authors.forename: M
authors.initials: M
authors.lastname: Pelikan-Filipek
mesh_terms: D000328:Adult; D001491:Basophils; D004804:Eosinophils; D004848:Epithelium; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000473:pathology*; D007958:Leukocyte Count; D008214:Lymphocytes; D008407:Mast Cells; D008875:Middle Aged; D009297:Nasal Mucosa / Q000378:metabolism*; D009299:Nasal Provocation Tests; D009504:Neutrophils; D012965:Sodium Chloride
chemical_list: D012965:Sodium Chloride