The sequence around the phosphorylation site of the porcine heart type phosphorylase isoenzyme.
1. A tetradecapeptide containing the phosphorylation site was obtained from 32P-labelled pig heart phosphorylase a isoenzyme by alpha-chymotryptic digestion. 2. The peptide was purified by Mono S cation-exchange chromatography and reversed-phase HPLC. 3. The phosphorylated residue was identified as Ser and the sequence was determined: T D G E R R K Q I S V R G L. 4. The sequence was compared to the known sequences of muscle and liver type isophosphorylases and the structural consequences of the amino acid residue exchanges were predicted.
Dombrádi
V
V
Institute of Medical Chemistry, University School of Medicine, Debrecen, Hungary.
Willis
A C
AC
Vereb
G
G
Johnson
L N
LN
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D007527:Isoenzymes / Q000378:metabolism*; D008099:Liver / Q000201:enzymology; D008969:Molecular Sequence Data; D009132:Muscles / Q000201:enzymology; D009206:Myocardium / Q000201:enzymology*; D010446:Peptide Fragments / Q000302:isolation & purification; D010762:Phosphorylase a / Q000378:metabolism*; D006005:Phosphorylases / Q000378:metabolism*; D010766:Phosphorylation; D013552:Swine
D007527:Isoenzymes; D010446:Peptide Fragments; D010762:Phosphorylase a; D006005:Phosphorylases