Identification by in situ hybridization of IFN-beta-producing murine macrophages obtained from high and low interferon producers.
Resident peritoneal macrophages obtained from If-1h and If-1l mice were induced in vitro with Newcastle disease virus. Then, 3, 5 and 7 h after induction, the cells were fixed and analysed for the presence of Mu IFN-beta mRNA by in situ hybridization, using a 35S-labelled Mu IFN-beta cDNA as a probe. The number of cells that were scored positive was the same in cultures derived from high responder If-1h and from low responder If-1l mice, and reached 100% of the cells present in the cultures. This result highly suggests that low responder mice have the same number of IFN-producing cells as high responders, and that the difference in production is not due to an increased number of producer cells in high responders.
De Maeyer-Guignard
J
J
Institut Curie-Biologie, Université de Paris-Sud, Orsay, France.
Marcucci
F
F
De Maeyer
E
E
D000818:Animals; D004247:DNA / Q000378:metabolism; D005809:Genes, Regulator; D066298:In Vitro Techniques; D007370:Interferon Type I / Q000096:biosynthesis* / Q000235:genetics; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D008815:Mice, Inbred Strains; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000378:metabolism
D007370:Interferon Type I; D012333:RNA, Messenger; D004247:DNA