[Passage of hybridomas in male BALB/c mice].
For cultivating hybridomas in the ascitic form there are usually used female mice BALB/c and not male ones. Efficiency of production of monoclonal antibodies with cultivation of the hybridomas in male and female mice BALB/c was studied comparatively. The animals were stimulated to form ascite by administration of the incomplete Freund's adjuvant or 3 per cent peptone with petrolatum oil. Some parameters of the ascite formation were studied: viability of the hybridoma cells, ascitic fluid formation period and volume, hybridoma cell concentration and titers of monoclonal antibodies in the ascitic fluid. In regard to all the parameters studied the male animals were not inferior to the female ones and in case of one of the hybridomas even surpassed them twofold by the volume of the ascitic fluid formed. This is evident of possible using male mice for mass cultivation of hybridoma cells with a purpose of obtaining preparative amounts of monoclonal antibodies in production of immunodiagnostic agents on their basis.
Novikov
V V
VV
Trofimova
M N
MN
Zhil'tsova
M A
MA
Biriukova
L Z
LZ
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D001202:Ascitic Fluid / Q000139:chemically induced / Q000276:immunology; D005260:Female; D005620:Freund's Adjuvant / Q000494:pharmacology; D006825:Hybridomas / Q000276:immunology*; D008297:Male; D008722:Methods; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology*; D010461:Peptones / Q000494:pharmacology; D012727:Sex Characteristics; D013997:Time Factors
D000911:Antibodies, Monoclonal; D010461:Peptones; D005620:Freund's Adjuvant
Passirovanie gibridom na samtsakh mysheĭ linii BALB/c.