Determination of (4-chlorophenyl)thiomethylene bisphosphonic acid, a new bisphosphonate, in biological fluids by high-performance liquid chromatography.
A rapid and sensitive high-performance liquid chromatographic method for the assay of the bisphosphonate (4-chlorophenyl)thiomethylene bisphosphonic acid in plasma and urine is described. It requires selective precipitations and dissolutions of calcium salts prior to reversed-phase chromatography with UV detection. This method used semi-micro scale material and 200-microliters biological aliquots. The limit for accurate quantification is 50 ng/ml. Data on reliability criteria and application to a pharmacokinetic study are presented.
Fels
J P
JP
Sanofi-Recherche, Montpellier, France.
Guyonnet
J
J
Berger
Y
Y
Cautreels
W
W
D002851:Chromatography, High Pressure Liquid; D004164:Diphosphonates / Q000032:analysis*; D006801:Humans; D013056:Spectrophotometry, Ultraviolet; D013997:Time Factors
D004164:Diphosphonates; C045541:SR 41319