Automation of data acquisition and processing in assays for anchorage-independent growth: application to the purification of epithelial transforming growth factor.
We have developed a method for automated data collection from anchorage-independent growth assays by direct interfacing of an Omnicon image analysis system with a VAX mainframe computer network. By use of this interface, data generated with the Omnicon can be acquired and manipulated by the VAX, providing several advantages including high throughput, elimination of operator error, flexibility and speed, and capacity of mainframe data processing. We have applied these techniques to aid in the purification of a novel growth factor for human epithelial cells. Both column elution profiles and dose-response data were processed to graphic formats, and ED50 values for the individual purification steps were obtained by Hill transformation of the dose-response curves. The assay for anchorage-independent growth is widely used for purification of growth factors and testing of chemotherapeutic agents against human tumor cells. The present technique should be useful in facilitating these labor-intensive studies.
Dunnington
D J
DJ
Department of Cell Biology, SmithKline & French Laboratories, King of Prussia, Pennsylvania 19406.
Pinsky
S
S
Mattes
D
D
Prichett
W
W
Earl
C Q
CQ
Greig
R
R
Anzano
M A
MA
D000818:Animals; D002417:Cattle; D002448:Cell Adhesion; D002455:Cell Division; D002851:Chromatography, High Pressure Liquid; D003114:Colony-Forming Units Assay; D003627:Data Interpretation, Statistical; D006801:Humans; D007668:Kidney / Q000032:analysis; D015291:Transforming Growth Factors / Q000302:isolation & purification*; D014410:Tumor Stem Cell Assay
D015291:Transforming Growth Factors