title: Expression of the proliferation markers Ki67 and transferrin receptor by human trophoblast populations.
abstract: Immunohistochemical techniques were used to investigate the expression of proliferation markers (Ki67 and transferrin receptor) by fetal trophoblast in normal human pregnancy. In placental villous tissue, transferrin receptor was detected not only on the apical syncytiotrophoblastic membrane but also on the proximal portion of cytotrophoblast columns, an area of high cellular proliferative activity. The majority of cells in cytotrophoblast columns and shell showed nuclear reactivity with Ki67. Villous syncytiotrophoblast was uniformly unreactive with Ki67 but a proportion of the underlying cytotrophoblast was Ki67-positive throughout pregnancy. Occasional Ki67-positive trophoblast cells were identified within chorion laeve at term. In contrast, interstitial and endovascular extravillous trophoblast in maternal uterine decidual tissue failed to label with either proliferation marker. Thus, chorionic villous cytotrophoblast and extravillous trophoblast in the chorion laeve appear to retain their proliferative capacity into late pregnancy. Cytotrophoblast columns represent a zone of cellular proliferation which may be dependent on transferrin.
authors.affiliation: Department of Pathology, University of Leeds, U.K.
authors.forename: J N
authors.initials: JN
authors.lastname: Bulmer
authors.forename: L
authors.initials: L
authors.lastname: Morrison
authors.forename: P M
authors.initials: PM
authors.lastname: Johnson
mesh_terms: D015415:Biomarkers; D002455:Cell Division; D002824:Chorionic Villi / Q000166:cytology / Q000378:metabolism; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D011247:Pregnancy; D011990:Receptors, Transferrin / Q000378:metabolism*; D014327:Trophoblasts / Q000166:cytology / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D011990:Receptors, Transferrin