The spleen colony technique. II. Errors in estimation of the fraction of CFU-s synthesizing DNA.
A refinement of the method for determining the fraction of CFU-s in the S phase is presented. The new procedures were tested by means of a model experiment in which the fraction of CFU-s 'killed' was simulated by diluting the cell suspension. Another test was to determine the fraction of the CFU-s in the S phase using duplicate samples.
Znojil
V
V
Department of Pathophysiology, Faculty of Medicine, Charles University, Prague, Czechoslovakia.
Necas
E
E
D000818:Animals; D003114:Colony-Forming Units Assay / Q000379:methods*; D004247:DNA / Q000096:biosynthesis; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D007399:Interphase; D051379:Mice; D008815:Mice, Inbred Strains; D013154:Spleen / Q000166:cytology*; D013936:Thymidine / Q000633:toxicity
D004247:DNA; D013936:Thymidine