Acid hydrolysis of serum samples to increase detection of HIV antigen.
Sera from 32 HIV-infected patients and 20 controls were assayed for HIV antigen (HIV-Ag) and antibodies following acid hydrolysis. Acid hydrolysis, followed by neutralization immediately prior to the assay, was found to be a simple means to solubilize immune complexes and allowed recovery of 40-50% of complexed antigen. Following acid hydrolysis, HIV-Ag levels rose or became detectable in 22/32 patients and HIV IgG1-4 levels rose in 17/20 patients studied. The results show that complexed HIV-Ag may evade detection in HIV-infected patients.
Mathiesen
T
T
Department of Virology, National Bacteriological Laboratory, Stockholm, Sweden.
Sundqvist
V A
VA
Albert
J
J
Ohlsson
E
E
Wahren
B
B
D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology*; D000936:Antigen-Antibody Complex / Q000032:analysis; D015483:HIV Antibodies / Q000032:analysis; D015488:HIV Antigens / Q000032:analysis*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007074:Immunoglobulin G / Q000032:analysis / Q000145:classification
D000936:Antigen-Antibody Complex; D015483:HIV Antibodies; D015488:HIV Antigens; D007074:Immunoglobulin G