title: Optimization of fluorescence detection for the determination of labetalol in plasma by high-performance liquid chromatography.
abstract: A sensitive reversed-phase high-performance liquid chromatographic method for the determination of labetalol has been developed. A mobile phase consisting of citrate buffer (pH 6.5), acetonitrile and 2-propanol and an RP-8 column were used. The sensitivity of the fluorescence detection was enhanced to 1 ng/ml of labetalol in plasma by optimizing the emitted light. General guidelines for optimization of fluorescence detection are discussed.
authors.affiliation: Drug Research Institute, Modra, Czechoslovakia.
authors.forename: V
authors.initials: V
authors.lastname: Ostrovská
authors.forename: X
authors.initials: X
authors.lastname: Svobodová
authors.forename: A
authors.initials: A
authors.lastname: Pechová
authors.forename: S
authors.initials: S
authors.lastname: Kusala
authors.forename: M
authors.initials: M
authors.lastname: Svoboda
mesh_terms: D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007741:Labetalol / Q000097:blood*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
chemical_list: D007741:Labetalol