Ascorbate increases apparent "specific" binding of [3H]pyrilamine to glass fiber filters.
Although filtration through glass fiber filters provides a convenient method for separating bound from unbound radioligand, binding of the ligand to the filters is often a problem. Ascorbate (0.1%) has been shown to decrease binding of serotonergic acids to these filters. In the present study, relatively high binding of [3H]pyrilamine, an H1-antagonist, to glass fiber filters was observed. The addition of ascorbate increased, rather than decreased, binding of [3H]pyrilamine to the filters. Thus, the effect of ascorbate on binding of radioligands to glass fiber filters appears to be dependent upon the particular ligand involved.
Williams
P B
PB
Department of Pharmacology, Eastern Virginia Medical School, Norfolk 23501.
Rosenthal
M D
MD
D000631:Aminopyridines / Q000302:isolation & purification*; D001205:Ascorbic Acid; D001665:Binding Sites; D002021:Buffers; D005374:Filtration / Q000295:instrumentation; D005898:Glass; D008024:Ligands; D011738:Pyrilamine / Q000302:isolation & purification*
D000631:Aminopyridines; D002021:Buffers; D008024:Ligands; D011738:Pyrilamine; D001205:Ascorbic Acid