title: High-performance liquid chromatographic analysis of ranitidine in plasma and urine.
abstract: A high-performance liquid chromatographic method for the analysis of ranitidine in plasma and urine is described. Plasma samples were extracted with dichloromethane while urine samples were injected directly after dilution. The mobile phase consisted of: 0.05 M ammonium acetate buffer containing 0.01 M octane sulphonate, 5.3%; methanol, 31.6%; and acetonitrile, 63.1%. Detection was carried out at 330 nm. Metoclopramide was used as the internal standard. Peak height ratios were measured. Absolute recovery from plasma was 83-85%. Within and between day coefficients of variation ranged from 0.79 to 2.42% and 1.09-2.95% respectively. Plasma and urine samples from a healthy volunteer were analysed.
authors.affiliation: Faculty of Pharmacy, Jordan University of Science and Technology, Irbid.
authors.forename: M
authors.initials: M
authors.lastname: Shiekh Salem
authors.forename: A M
authors.initials: AM
authors.lastname: Gharaibeh
authors.forename: H N
authors.initials: HN
authors.lastname: Alkaysi
authors.forename: A
authors.initials: A
authors.lastname: Badwan
mesh_terms: D002021:Buffers; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D008787:Metoclopramide / Q000032:analysis; D011899:Ranitidine / Q000032:analysis* / Q000097:blood / Q000652:urine; D012015:Reference Standards
chemical_list: D002021:Buffers; D011899:Ranitidine; D008787:Metoclopramide