[Complementary-addressed elimination of E1a sequence of simian adenovirus oncogene SA7 from circular single-stranded DNA of recombinant phage M13].
The G fragment of the simian adenovirus SA7 oncogene corresponding to E1a region was cloned into M13mp8 and M13mp9 phages. Single-stranded DNAs of the recombinant phages thus obtained (mp8G and mp9G) partially digested with DNAse II were used to synthesize polyalkylating derivatives capable of specific hybridisation and subsequent alkylation of complementary G sequences of corresponding phage DNAs. After incubation of complementary alkylated DNA in the presence of lysine, the preselected region (G fragment) was specifically eliminated without damaging vector sequences. The method of complementary-addressed cleavage proved to be useful for precise analysis of reactions of polyalkylating derivatives within complementary complexes.
Sats
N V
NV
Surin
V L
VL
Zhukova
E L
EL
Grineva
N I
NI
D000256:Adenoviridae / Q000235:genetics*; D000261:Adenoviruses, Simian / Q000235:genetics*; D000478:Alkylation; D001435:Bacteriophages / Q000235:genetics*; D004270:DNA, Circular / Q000235:genetics; D004277:DNA, Single-Stranded / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D009857:Oncogenes; D011995:Recombination, Genetic
D004270:DNA, Circular; D004277:DNA, Single-Stranded; D004279:DNA, Viral
Komplementarno adresovannoe éliminirovanie E1a-posledovatel'nosteĭ onkogena adenovirusa obez'ian SA7 iz kol'tsevykh odnotiazhevykh DNK rekombinantnykh fagov M13.