Egasyn affects the processing of beta-glucuronidase in mouse liver.
Three differently modified forms of beta-glucuronidase are known to exist: a microsomal enzyme form (M) existing in tissues where egasyn, a second microsomal protein, is present; and an acidic (La; complex-type oligosaccharide) and a basic (Lb; non-complex type oligosaccharide) lysosomal form which occur in all mouse tissues. Lb predominates in tissues containing microsomal beta-glucuronidase, La in those lacking it. In pulse-labelling experiments using mouse strain C57BL/6 liver containing egasyn (Eg+/Eg+) and microsomal enzyme, about half of the newly synthesized beta-glucuronidase was processed to the microsomal enzyme form, which was evidently further processed to Lb, and about half directly to La. In contrast, in liver of the congenic line C57BL/6.YBR Es-1b Eg0 that lacks egasyn (Eg0/Eg0) and microsomal enzyme, most of the labelled beta-glucuronidase was processed to La, and only a minor portion to Lb. Newly synthesized enzyme appeared first in microsomal, then in light and heavy lysosomal fractions of Eg+/Eg+ liver. In Eg0/Eg0 liver, no labelled enzyme was measurable in the microsomes, but it appeared rapidly in both types of lysosomes. Taken together these findings indicate that the microsomal enzyme form serves as a precursor of Lb, and that La is synthesized independently. The apparent half-life of La is only two-thirds that of Lb; this fact accounts for the reduced beta-glucuronidase activity in Eg0/Eg0 liver, which contains La as the predominant form.
Pfister
K
K
Department of Pediatrics, University of Zürich, Switzerland.
Bosshard
N
N
Zopfi
M
M
Gitzelmann
R
R
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002265:Carboxylic Ester Hydrolases; D002458:Cell Fractionation; D005260:Female; D005966:Glucuronidase / Q000378:metabolism*; D007424:Intracellular Fluid / Q000187:drug effects / Q000378:metabolism; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000378:metabolism*; D007930:Leucine / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D008562:Membrane Glycoproteins / Q000494:pharmacology*; D051379:Mice; D008810:Mice, Inbred C57BL; D013347:Subcellular Fractions / Q000187:drug effects / Q000378:metabolism
D007527:Isoenzymes; D008562:Membrane Glycoproteins; D002265:Carboxylic Ester Hydrolases; C009751:egasyn; D005966:Glucuronidase; D007930:Leucine