title: Pancreatic cancer mucin from xenografts of SW1990 cells: isolation, characterization, and comparison to colon cancer mucin.
abstract: Mucin has been purified from nude mouse xenografts of SW1990 human pancreatic cancer cells. The mucin was eluted at the void volume of Sepharose CL-4B and was of density greater than 1.3 in CsCl gradients. The isolated mucin had a high content of threonine, serine, and proline, with 31% of the amino acid residues O-glycosylated. The average oligosaccharide composition was NeuAc1.8Fuc0.7Gal2.0GlcNAc1.7GalNAc1.4. Polyclonal rabbit antibodies prepared against the purified mucin recognized primarily mucin polypeptide, and there was extensive immunological cross-reaction between SW1990 pancreatic cancer mucin and LS174T colon cancer mucin. However, using carbohydrate-specific monoclonal antibodies, the two mucins were found to differ. SW1990 mucin had more Lewis, sialyl Lewis, and sialyl Lewis activity, while the colon cancer mucin had more sialyl T antigen. Since pancreatic mucins, whether from normal pancreas or pancreatic cancer, have not previously been well characterized, the availability of SW1990 pancreatic cancer mucin may be useful as a model for studying the expressing of organ-specific or cancer-associated antigens.
authors.affiliation: Gastrointestinal Research Laboratory, Veterans Administration Medical Center, San Francisco, California 94121.
authors.forename: J
authors.initials: J
authors.lastname: Nardelli
authors.forename: J C
authors.initials: JC
authors.lastname: Byrd
authors.forename: J
authors.initials: J
authors.lastname: Ho
authors.forename: F J
authors.initials: FJ
authors.lastname: Fearney
authors.forename: C
authors.initials: C
authors.lastname: Tasman-Jones
authors.forename: Y S
authors.initials: YS
authors.lastname: Kim
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000032:analysis; D002460:Cell Line; D003110:Colonic Neoplasms / Q000032:analysis* / Q000276:immunology; D003429:Cross Reactions; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009077:Mucins / Q000032:analysis*; D010190:Pancreatic Neoplasms / Q000032:analysis* / Q000276:immunology; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured
chemical_list: D000906:Antibodies; D015295:Antigens, Tumor-Associated, Carbohydrate; D009077:Mucins