Quantitative in vitro autoradiographic characterization of [125I]angiotensin III binding sites in rat adrenal gland.
A single class of high-affinity binding sites for [125I]angiotensin III and [125I]angiotensin II were found in rat adrenal medulla and zona glomerulosa by quantitative autoradiography. In the medulla, Kd were 1.46 and 1.16 nM, and Bmax 1700 and 1700 fmol/mg protein, for [125I]angiotensin II and [125I]angiotensin III, respectively. In the zona glomerulosa, Kd were 0.86 and 0.90 nM, and Bmax 790 and 560 fmol/mg protein, for [125I]angiotensin II and [125I]angiotensin III, respectively. Unlabeled angiotensin III and angiotensin II displaced [125I]angiotensin III with similar potency in both adrenal zona glomerulosa and medulla. Our findings suggest that angiotensin III and angiotensin II might share the same binding sites in adrenal gland and support the hypothesis of a role for angiotensin III in the adrenal medulla and zona glomerulosa.
Himeno
A
A
Unit on Preclinical Neuropharmacology, National Institute of Mental Health, Bethesda, MD 20892.
Nazarali
A J
AJ
Saavedra
J M
JM
D000313:Adrenal Medulla / Q000032:analysis*; D000804:Angiotensin II / Q000031:analogs & derivatives* / Q000032:analysis; D000805:Angiotensin III / Q000032:analysis*; D000818:Animals; D001345:Autoradiography; D006801:Humans; D007457:Iodine Radioisotopes; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D011945:Receptors, Angiotensin / Q000032:analysis*; D015384:Zona Glomerulosa / Q000032:analysis*
D007457:Iodine Radioisotopes; D011945:Receptors, Angiotensin; D000804:Angiotensin II; D000805:Angiotensin III