A comparison of recovery of virus from wastewaters by beef extract-Celite, ferric chloride, and filter concentration procedures.
An improved concentration method using sample volumes as large as 1500 ml has been developed to monitor for viruses in wastewaters. Non-precipitating dry beef extract powder is added to wastewater samples to give a 3% concentration and mixed until dissolved. This is followed by the addition of Celite as a virus adsorbent. By manipulating pH, viruses are eluted from the Celite in small volumes of phosphate buffer. This procedure was further tested without the aid of the Celite additives using a precipitating beef extract powder and substituting FeCl3 as an alternate reagent for the Celite. Comparison testing was also made with the currently recommended cartridge and disc filter procedures. In all cases, the non-precipitating beef extract-Celite method gave higher recovery rates in highly polluted waters.
Dahling
D R
DR
Virology Section, U.S. Environmental Protection Agency, Cincinnati, Ohio 45268.
Wright
B A
BA
D000327:Adsorption; D000818:Animals; D002417:Cattle; D002712:Chlorides; D007692:Diatomaceous Earth; D005069:Evaluation Studies as Topic; D005290:Ferric Compounds; D005374:Filtration; D008461:Meat Products; D012722:Sewage; D014780:Viruses / Q000302:isolation & purification*; D014865:Waste Disposal, Fluid; D014871:Water Microbiology
D002712:Chlorides; D005290:Ferric Compounds; D012722:Sewage; D007692:Diatomaceous Earth; C024555:ferric chloride