Evidence that the phagocytosis mediated by the peanut agglutinin-like activity of IgG(Fc) receptors of human monocytes is selectively modulated by estradiol and natural estrogens.
The percentage of human monocytes (MCs) that are able to form rosettes with, and to phagocytose, IgG-coated sheep red blood cells (IgG-SRBCs) has been first determined in vitro by a classical rosette assay in 12 postmenopausal (PM) women. Half of them never received any suppletive estrogen (E) therapy at the time of testing, whereas the other six were chronically treated with E. Three different preparations of the same anti-SRBC IgG antibody batch were coated to SRBCs: the first one was the starting antibody preparation [IgG(total] and the other two were purified by affinity chromatography either on Sepharose-concanavalin A (Con A) or on agarose-peanut agglutinin (PNA) columns specifically recognizing terminal, and/or accessible, alpha-mannosyl [IgG(Con A)] or beta-galactosyl [IgG(PNA)] residues of the Fc domain, respectively. The three IgG preparations exhibited similar hemagglutinating antibody titers (1/100). All experiments were conducted using a coating range of 5000 to 6000 IgG antibody molecules per SRBC. In PM women with E, the rosetting capacity of autologous MCs (percentage of MCs rosetting at least three IgG-SRBCs), their phagocytosing capacity (percentage of MCs ingesting at least three IgG-SRBCs), and the phagocytosis index (number of SRBCs ingested/100 MCs) were similar for each IgG-SRBC preparation considered. In contrast, in PM women without E, the capacity of MCs to phagocytose IgG(PNA)-SRBCs, as well as the phagocytosis index measured with those SRBCs, was strongly reduced (P less than 0.01 at least), when compared to the same parameters determined using IgG(total)-SRBCs and IgG(Con A)-SRBCs.(ABSTRACT TRUNCATED AT 250 WORDS)
Malaise
M G
MG
Department of Medicine, Chu Sart-Tilman, Liège, Belgium.
Franchimont
P
P
Mahieu
P R
PR
D000328:Adult; D000368:Aged; D004958:Estradiol / Q000008:administration & dosage* / Q000502:physiology; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology*; D037102:Lectins / Q000276:immunology; D008593:Menopause / Q000276:immunology*; D008598:Menstruation; D008875:Middle Aged; D009000:Monocytes / Q000276:immunology*; D019887:Peanut Agglutinin; D010587:Phagocytosis; D011897:Random Allocation; D011961:Receptors, Fc / Q000276:immunology*; D012397:Rosette Formation
D007074:Immunoglobulin G; D037102:Lectins; D019887:Peanut Agglutinin; D011961:Receptors, Fc; D004958:Estradiol