title: Association of thrombospondin of endothelial cells with other matrix proteins and cell attachment sites and migration tracks.
abstract: Different biochemical and cytochemical techniques were applied to characterize the sites of localization of thrombospondin in cultured endothelial cells. The results obtained by [35S]methionine labeling, immunoblotting, immunoprecipitation, fluorescence microscopy, ultracytochemistry, immunogold labeling, and silver enhancement experiments revealed that thrombospondin secreted by endothelial cells is structurally organized together with proteoheparan sulfate in spherical granules at the cell surface. These granules are about 100 to 300 nm in size. Heparin or enzymatic degradation with heparitinase, but not with ABC lyase, release thrombospondin from the cell surface. Fibronectin is expressed in the extracellular matrix of endothelial cells in a fibrillar organization, clearly distinct from the punctate pattern of thrombospondin on the cell surface. Furthermore, secreted thrombospondin is highly enriched together with fibronectin and proteoheparan sulfate in cell attachment sites and in cell migration tracks. In cell migration tracks proteoheparan sulfate more clearly resembles the fibrillar distribution pattern of fibronectin, whereas thrombospondin reveals a rather monodisperse pattern. The obtained data suggest preferential sites of interaction between thrombospondin and heparan sulfate proteoglycans on the cell surface and a participation of thrombospondin in cell adhesion and cell migration.
authors.affiliation: Institut für Arterioskleroseforschung, Universität, Münster/Bundesrepublik Deutschland.
authors.forename: P
authors.initials: P
authors.lastname: Vischer
authors.forename: W
authors.initials: W
authors.lastname: Völker
authors.forename: A
authors.initials: A
authors.lastname: Schmidt
authors.forename: N
authors.initials: N
authors.lastname: Sinclair
mesh_terms: D000818:Animals; D001011:Aorta / Q000166:cytology; D002448:Cell Adhesion; D002465:Cell Movement; D002478:Cells, Cultured; D011508:Chondroitin Sulfate Proteoglycans / Q000378:metabolism; D004730:Endothelium, Vascular / Q000166:cytology* / Q000378:metabolism; D005109:Extracellular Matrix / Q000032:analysis / Q000378:metabolism / Q000648:ultrastructure; D005353:Fibronectins / Q000378:metabolism; D006023:Glycoproteins / Q000032:analysis / Q000276:immunology / Q000378:metabolism*; D006025:Glycosaminoglycans / Q000378:metabolism; D019812:Heparan Sulfate Proteoglycans; D006493:Heparin / Q000494:pharmacology; D006497:Heparan Sulfate / Q000378:metabolism; D006651:Histocytochemistry; D006801:Humans; D015151:Immunoblotting; D008562:Membrane Glycoproteins / Q000378:metabolism*; D008854:Microscopy, Electron; D011133:Polysaccharide-Lyases / Q000378:metabolism; D011233:Precipitin Tests; D013552:Swine; D019699:Thrombospondins
chemical_list: D011508:Chondroitin Sulfate Proteoglycans; D005353:Fibronectins; D006023:Glycoproteins; D006025:Glycosaminoglycans; D019812:Heparan Sulfate Proteoglycans; D006493:Heparin; D006497:Heparan Sulfate; D008562:Membrane Glycoproteins; D011133:Polysaccharide-Lyases; D019699:Thrombospondins; C021349:heparitinsulfate lyase