Development of immunoradiometric assays for human thyroglobulin using monoclonal antibodies and the biotin/avidin system.
Monoclonal antibodies against human thyroglobulin were produced by the hybridoma technique. Using three non-crossreactive monoclonal antibodies, an IRMA system was developed, with a polyclonal rabbit antibody fixed on microtiter plates as first extracting antibody. The monoclonal antibodies were used as second antibody, while anti-mouse IgG-biotin from goat and [125I]streptavidin served as the indicator system. The source of the monoclonal antibodies was diluted culture medium without purification. Sensitivities of 3-4 micrograms/l were obtained with all 3 monoclonal antibodies; interassay variation was about 5%. This test system will be used for further immunological characterization of circulating thyroglobulin in different thyroid diseases.
Pfahl
H
H
Medizinische Klinik, Universität Heidelberg, Mannheim.
Heilig
B
B
Bethäuser
H
H
Hüfner
M
M
Schmidt-Gayk
H
H
Junker
M
M
D000911:Antibodies, Monoclonal; D001360:Avidin; D001710:Biotin; D003429:Cross Reactions; D006801:Humans; D007202:Indicators and Reagents; D011863:Radioimmunoassay / Q000379:methods; D013954:Thyroglobulin / Q000097:blood* / Q000276:immunology
D000911:Antibodies, Monoclonal; D007202:Indicators and Reagents; D001360:Avidin; D001710:Biotin; D013954:Thyroglobulin