Peptide-specific antibodies employed in determining the interspecies immunological cross-reactivity of haemopexin.
1. Antibodies were raised in rabbits against nine peptides analogous to sequences of the human serum beta-glycoprotein haemopexin, and seven peptides were very antigenic. 2. One of these affinity-purified peptide-specific antibodies interacted with a highly conserved sequence of the haemopexin of five of the seven species tested. 3. Another antibody bound pig haemopexin even better than human haemopexin. 4. The overall, arbitrarily assessed, immunological cross-reactivity between the haemopexin of human and other species follows the order: rabbit greater than mouse greater than chicken greater than pig greater than rat greater than cow.
Cheng
K C
KC
Department of Cell Biology, Cornell University Medical College, NY 10021.
De Falco
M
M
Maloy
W L
WL
Liem
H H
HH
Muller-Eberhard
U
U
D000818:Animals; D000918:Antibody Specificity; D002417:Cattle; D002645:Chickens; D003429:Cross Reactions; D006466:Hemopexin / Q000276:immunology*; D006801:Humans; D007120:Immunochemistry; D051379:Mice; D010446:Peptide Fragments / Q000276:immunology; D011487:Protein Conformation; D011817:Rabbits; D051381:Rats; D013045:Species Specificity; D013552:Swine
D010446:Peptide Fragments; D006466:Hemopexin