Trehalose and dry dipalmitoylphosphatidylcholine revisited.
Dry mixtures of sonicated vesicles of DPPC and trehalose which contained a maximum of 0.2 mol water/mol lipid were examined by differential scanning calorimetry, Fourier transform infrared spectroscopy and freeze-fracture electron microscopy. Samples of dry DPPC and trehalose prepared from aqueous solution had a minimum Tm of 24 degrees C for the gel to liquid-crystalline transition provided that the vesicles were dried with trehalose while the lipid was in liquid-crystalline phase. This low transition is compared to a transition of 105-112 degrees C for dry pure DPPC and of 42 degrees C for hydrated pure DPPC. The present work is an extension of earlier work from this laboratory using both other lipids and other methods of preparation.
Crowe
L M
LM
Department of Zoology, University of California, Davis 95616.
Crowe
J H
JH
D015060:1,2-Dipalmitoylphosphatidylcholine; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004187:Disaccharides; D005583:Fourier Analysis; D005614:Freeze Fracturing; D005782:Gels; D008854:Microscopy, Electron; D013055:Spectrophotometry, Infrared; D013696:Temperature; D014199:Trehalose; D014867:Water
D004187:Disaccharides; D005782:Gels; D014867:Water; D015060:1,2-Dipalmitoylphosphatidylcholine; D014199:Trehalose