Isolation and fluorometric determination of homovanillic acid in urine and tissues.
The fluorometric method is described for determination of the final product of dopamine metabolism homovanillic acid in urine, amniotic fluid and tissue extracts. The metabolite was isolated using three chromatographic columns (1-Amberlit CG-50, 2-Dowex AG 1 x 2, 8-Aluminium oxide). Oxidation of eluates to emitter was done with potassium ferricyanide in alkalic medium. The use of internal standards made high recovery of compound and a good specificity and sensitivity of detection.
Maruchin
J E
JE
Department of Biochemistry, Medical Center of Postgraduate Education, Warsaw.
Olesiński
J
J
D000653:Amniotic Fluid / Q000032:analysis*; D005260:Female; D005470:Fluorometry / Q000379:methods; D006719:Homovanillic Acid / Q000032:analysis* / Q000652:urine; D006801:Humans; D011247:Pregnancy; D014020:Tissue Extracts / Q000032:analysis
D014020:Tissue Extracts; D006719:Homovanillic Acid