Modulation of lymphocyte and monocyte responses in vitro by 9-deoxy-delta 9-prostaglandin D2 and 9-deoxy-delta 9-delta 12-prostaglandin D2.
The effects of 9-deoxy-delta 9-prostaglandin D2 (PGJ2) and 9-deoxy-delta 9-delta 12-prostaglandin D2 (delta 12PGJ2), which are metabolites of PGD2, on lymphocyte and monocyte reactions were studied in vitro. Expression of various phenotypic markers of lymphocyte subsets, as detected by monoclonal antibodies, was not affected by overnight incubation in 3 x 10(-5) M PGJ2. Phytohemagglutinin stimulation and natural killer activity of lymphocytes was reduced by PGJ2 and delta 12 PGJ2. Monocyte reactivity to 12-o-tetradecanoylphorbol-13-acetate, as assessed by chemiluminescence, was stimulated by preincubation of the cells for 1 h in 3 x 10(-5) M PGJ2 or delta 12 PGJ2. Such an augmentation was not exerted by PGD2.
Petrini
B
B
Central Microbiological Laboratory, Stockholm County Council, Sweden.
Wasserman
J
J
Hammarström
S
S
Blomgren
H
H
Vedin
I
I
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000943:Antigens, Differentiation / Q000032:analysis; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D006801:Humans; D007694:Killer Cells, Natural / Q000187:drug effects; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000145:classification / Q000187:drug effects*; D008262:Macrophage Activation / Q000187:drug effects; D009000:Monocytes / Q000187:drug effects*; D015230:Prostaglandin D2 / Q000031:analogs & derivatives* / Q000494:pharmacology
D000276:Adjuvants, Immunologic; D000943:Antigens, Differentiation; C037112:9-deoxy-delta-9-prostaglandin D2; C040810:9-deoxy-9,10-didehydro-12,13-didehydro-13,14-dihydroprostaglandin D2; D015230:Prostaglandin D2