title: Enumeration of virulent Yersinia enterocolitica colonies by DNA colony hybridization using alkaline treatment and paper filters.
abstract: The efficiency of enumerating virulent Yersinia enterocolitica colonies was determined for 10 artificially contaminated foods by autoradiography after DNA colony hybridization using a 32P-labelled genetic probe and paper filters. Enumeration efficiency, which ranged from 0-98% (average 25 +/- 31% standard deviation), improved (42-138%, average 83 +/- 27% standard deviation) after enrichment with 0.5% KOH-0.5% NaCl. These efficiencies are equivalent to those obtained with nitrocellulose filters.
authors.affiliation: Center for Microbiological Investigations, Food and Drug Administration, Minneapolis, MN 55401.
authors.forename: J A
authors.initials: JA
authors.lastname: Jagow
authors.forename: W E
authors.initials: WE
authors.lastname: Hill
mesh_terms: D001345:Autoradiography / Q000379:methods; D004269:DNA, Bacterial / Q000235:genetics*; D005516:Food Microbiology; D006863:Hydrogen-Ion Concentration; D009693:Nucleic Acid Hybridization; D010209:Paper; D010761:Phosphorus Radioisotopes; D015008:Yersinia enterocolitica / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D004269:DNA, Bacterial; D010761:Phosphorus Radioisotopes