Quantification of hemoglobin A2 and identification of hemoglobin variants using a fully automated hemoglobin analyzer.
The DIAMAT Analyser System is a fully automated high performance liquid chromatographic (HPLC) instrument originally designed for the quantification of glycated hemoglobin (HbA1c). Buffers were developed for the separation and quantification of hemoglobin A2 on the DIAMAT. Also studied were the retention patterns of various hemoglobin variants on the DIAMAT using the buffers developed for the hemoglobin A2 quantification.
Bruegger
B B
BB
BioRad Laboratories, Hercules, California.
Keenan
D H
DH
Sivorinovsky
G
G
D000328:Adult; D002851:Chromatography, High Pressure Liquid; D006441:Hemoglobin A / Q000032:analysis*; D006443:Hemoglobin A2 / Q000032:analysis*; D006455:Hemoglobins, Abnormal / Q000032:analysis*; D006801:Humans; D012015:Reference Standards
D006455:Hemoglobins, Abnormal; D006441:Hemoglobin A; D006443:Hemoglobin A2