In vitro maintenance of Onchocerca volvulus for harvest of excretory and secretory products.
Recommendations are given for maintenance of female Onchocerca volvulus; conditions as standardized here for drug screening and harvest of secreted-excreted products resulted in survival of worms in culture medium for up to four and six weeks in absence and presence of serum, respectively. As parameter for viability of worms lactate excretion was determined beside control of motility.
Walter
R D
RD
Department of Biochemistry, Bernhard Nocht Institute for Tropical Medicine, Hamburg, FRG.
D000818:Animals; D003470:Culture Media; D005260:Female; D007773:Lactates / Q000032:analysis; D009854:Onchocerca / Q000032:analysis / Q000502:physiology*; D011506:Proteins / Q000032:analysis
D003470:Culture Media; D007773:Lactates; D011506:Proteins