title: Growth and repair of cartilage: organ culture system utilizing chondroprogenitor cells of condylar cartilage in newborn mice.
abstract: The zone of progenitor cells of mandibular condyles of neonatal mice was kept in an organ culture system for up to 8 days. Qualitative and quantitative determinations indicated a pronounced proliferative activity during the initial phases of the culture followed by a differentiation phase and the acquisition of typical hyaline cartilage. The mature hypertrophic chondrocytes were found to be surrounded by cartilage-specific macromolecules such as type II collagen, cartilage proteoglycans, and cartilage anchorin. The extracellular mineralization proceeded along matrix vesicles as is usually noted in vivo. A unique finding in this study was the observation that explants comprising cartilage progenitor cells and their adjacent extracellular matrix succeeded in repairing the damaged condylar in vitro.
authors.affiliation: Rappaport Family Institute for Research in the Medical Sciences, Faculty of Medicine Technion-Israel Institute of Technology, Haifa.
authors.forename: A
authors.initials: A
authors.lastname: Weiss
authors.forename: E
authors.initials: E
authors.lastname: Livne
authors.forename: K
authors.initials: K
authors.lastname: von der Mark
authors.forename: D
authors.initials: D
authors.lastname: Heinegard
authors.forename: M
authors.initials: M
authors.lastname: Silbermann
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002358:Cartilage, Articular / Q000166:cytology* / Q000648:ultrastructure; D002455:Cell Division; D005109:Extracellular Matrix / Q000648:ultrastructure; D007700:Kinetics; D008335:Mandibular Condyle; D051379:Mice; D008813:Mice, Inbred ICR; D008854:Microscopy, Electron; D009924:Organ Culture Techniques