title: Determination of 17-hydroxyprogesterone in plasma by gas chromatography-mass spectrometry with high-resolution selected-ion monitoring.
abstract: A method for determining 17-hydroxyprogesterone in plasma by isotope dilution-mass spectrometry is described. For the internal standard 17-hydroxy [2H4]progesterone is used. Extraction of plasma is followed by conversion into the 3,20-dienol,17-tristrimethylsilyl ether derivative and analysis by capillary gas chromatography-mass spectrometry with selected-ion monitoring, at a resolution of 6000. The lower limit of quantitation was 1 pg, judged from a criterion of a signal-to-noise ratio of 10. The precision and accuracy of the method were satisfactory.
authors.affiliation: Division of Biochemistry, Tottori University School of Medicine, Yonago, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Shimizu
authors.forename: T
authors.initials: T
authors.lastname: Hara
authors.forename: N
authors.initials: N
authors.lastname: Yamaga
authors.forename: H
authors.initials: H
authors.lastname: Kohara
authors.forename: K
authors.initials: K
authors.lastname: Nojima
mesh_terms: D019326:17-alpha-Hydroxyprogesterone; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D006908:Hydroxyprogesterones / Q000097:blood*; D007202:Indicators and Reagents; D007553:Isotope Labeling; D014297:Trimethylsilyl Compounds / Q000097:blood / Q000138:chemical synthesis
chemical_list: D006908:Hydroxyprogesterones; D007202:Indicators and Reagents; D014297:Trimethylsilyl Compounds; D019326:17-alpha-Hydroxyprogesterone