The influence of thyroid hormones on colony growth of peripheral CFU-GM from normal and leukemic subjects.
In order to clarify the alterations of granuloblastic cells in chronic and acute myeloid leukemia, the colony growth behavior of cultured CFU-GM from the peripheral blood of normal and leukemic subjects was examined in basal conditions and after adding to the medium T3 or T4 and/or thioproline and/or flurbiprofen. These drugs had in previous investigations proved their ability to modify cellular receptors and the uptake of thyroid hormones. The study was carried out in semisolid (double agar layer) and liquid medium, utilizing the techniques described previously. Both thyroid hormones enhanced the colony growth from normal peripheral blood CFU-GM and the response was more evident with T4 than T3. The effect on leukemic CFU-GM (from CML and AML) was less clear, probably due to the presence in leukemic cells of a defect of cellular uptake and to the utilization of T3 and T4. Indeed, on addition to the culture medium of thioproline, which modifies membrane permeability, and of fluorbiprofen, which inhibits PGE synthesis, the colony number and growth from leukemic CFU increased considerably in accordance with the results of our previous studies on these substances showing that they are able to modify cellular receptors for thyroid and several other hormones.
Notario
A
A
Department of Internal Medicine and Medical Therapy, University of Pavia.
Torriani
A
A
Bravi
M
M
Broglia
M
M
Borghi
G
G
Guerra
G
G
D002478:Cells, Cultured; D005480:Flurbiprofen / Q000494:pharmacology; D006098:Granulocytes / Q000187:drug effects*; D006412:Hematopoietic Stem Cells / Q000187:drug effects*; D006801:Humans; D007938:Leukemia / Q000097:blood*; D009000:Monocytes / Q000187:drug effects*; D013844:Thiazoles / Q000494:pharmacology; D053778:Thiazolidines; D013963:Thyroid Hormones / Q000494:pharmacology*
D013844:Thiazoles; D053778:Thiazolidines; D013963:Thyroid Hormones; D005480:Flurbiprofen; C003438:thiazolidine-4-carboxylic acid