Cloning and sequence analysis of a cDNA from seminal vesicle tissue encoding the precursor of the major protein of bull semen.
A cDNA library derived from poly(A)+RNA of bull seminal vesicle tissue was screened with a synthetic DNA probe specific for the major protein of bull semen. A positive clone pMP17, containing a 680-bp insert, was sequenced. In combination with primer-extension sequencing of poly(A)+RNA, a DNA sequence of 700 bp was determined. This DNA encodes a reading frame for 134 amino acids, starting with an ATG and terminated by a TAG codon. The first 25 amino acids constitute a signal peptide segment followed by 109 amino acids with the known sequence of the major protein. The initiation methionine occurs within the sequence CTACCATGG, which is highly homologous to a putative control signal for translational efficiency of mammalian mRNAs. The DNA sequence comprises a 3' untranslated region of 276 bp and the polyadenylation signal AATAAA, 13 bp upstream from a tract of A residues. Northern analysis indicated the presence of a 750-bp mRNA species in poly(A)+RNA of seminal vesicle tissue. According to Southern analysis, one gene appears to specify the major protein of bull semen.
Kemme
M
M
Max-Planck-Institut für Biophysikalische Chemie, Abt. Molekulare Biologie, Göttingen, W. Germany.
Scheit
K H
KH
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis / Q000235:genetics*; D008297:Male; D029584:Prostatic Secretory Proteins; D011498:Protein Precursors / Q000235:genetics*; D011506:Proteins / Q000235:genetics*; D029589:Seminal Plasma Proteins; D012669:Seminal Vesicles / Q000378:metabolism*
D029584:Prostatic Secretory Proteins; D011498:Protein Precursors; D011506:Proteins; D029589:Seminal Plasma Proteins; C043076:beta-microseminoprotein; D004247:DNA