A new method for the unidirectional deletion of DNA with Bal 31 nuclease using 2'-O-MeRNA-DNA chimeric adaptors.
We have developed a new procedure for unidirectional DNA deletion with Bal 31 nuclease. This method is based on the use of chimeric adaptors containing double strands of 2'-O-methyl RNA to block against an attack of Bal 31 nuclease. This contributes a wide application to learn the relation of structure-function of genes.
Mukai
S
S
Central Research Laboratories, Ajinomoto Co., Inc., Kawasaki, Japan.
Shibahara
S
S
Morisawa
H
H
D002678:Chimera; D002872:Chromosome Deletion; D004247:DNA / Q000235:genetics*; D004706:Endodeoxyribonucleases; D005796:Genes; D005818:Genetic Engineering / Q000379:methods; D008745:Methylation; D012330:RNA, Double-Stranded
D012330:RNA, Double-Stranded; D004247:DNA; D004706:Endodeoxyribonucleases; C038407:exonuclease Bal 31