Interaction of basic extension peptide fragments of adrenodoxin precursor with phospholipid vesicles.
Two extension peptide fragments PA1-4 and PA17-32, which correspond to the residues 1-14 and 17-32, respectively, of adrenodoxin precursor, were synthesized by the solution method to find a sequence necessary for the import of the precursor into mitochondria. Biological assay showed that PA1-14 inhibited the import of two mitochondrial enzyme precursors, but PA17-32 showed no inhibition, indicating that the N-terminal sequence has important information for import. CD spectra of the peptides demonstrated that PA1-14 formed alpha-helical structure in Tris-HCl buffer (pH 7.4) containing acidic phospholipid liposomes. Furthermore, PA1-14 induced the moderate leakage of carboxyfluorescein from phospholipid vesicles. The relationship between the structure and function of the peptides is discussed.
Aoyagi
H
H
Laboratory of Biochemistry, Faculty of Science, Kyushu University, Fukuoka, Japan.
Lee
S
S
Nakamura
H
H
Park
N G
NG
Kato
T
T
D015060:1,2-Dipalmitoylphosphatidylcholine; D000302:Adrenal Cortex / Q000648:ultrastructure; D000325:Adrenodoxin / Q000378:metabolism*; D000818:Animals; D001419:Bacteria / Q000187:drug effects; D001681:Biological Assay; D001692:Biological Transport / Q000187:drug effects; D002417:Cattle; D002786:Cholesterol Side-Chain Cleavage Enzyme / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D002942:Circular Dichroism; D005452:Fluoresceins / Q000378:metabolism; D005456:Fluorescent Dyes; D008081:Liposomes / Q000378:metabolism*; D008928:Mitochondria / Q000378:metabolism; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000378:metabolism* / Q000494:pharmacology; D010715:Phosphatidylglycerols; D011487:Protein Conformation; D011498:Protein Precursors / Q000378:metabolism*; D013329:Structure-Activity Relationship
D005452:Fluoresceins; D005456:Fluorescent Dyes; D008081:Liposomes; D010446:Peptide Fragments; D010715:Phosphatidylglycerols; D011498:Protein Precursors; D000325:Adrenodoxin; D015060:1,2-Dipalmitoylphosphatidylcholine; C024098:6-carboxyfluorescein; D002786:Cholesterol Side-Chain Cleavage Enzyme; C030345:1,2-dipalmitoylphosphatidylglycerol