Effect of ornithine on transferrin secretion of rat and human hepatocyte cultures.
Fetal rat or neonatal human hepatocytes and a human hepatoma cell line were cultured in an arginine-free medium, supplemented or not with L-ornithine. This amino-acid improved survival of hepatocytes and strongly enhanced their transferrin secretion. Moreover, this increase observed in transferrin production was well correlated with a higher corresponding mRNA level. Thus, it may be postulated that the mechanism involved in the increased transferrin secretion by L-ornithine is of pretranslational origin.
Lescoat
G
G
Unité de Recherches Hépatologiques U 49 de l'INSERM, Hôpital de Pontchaillou, Rennes, France.
Loreal
O
O
Desvergne
B
B
Pasdeloup
N
N
Deugnier
Y
Y
Brissot
P
P
D000818:Animals; D002478:Cells, Cultured; D005333:Fetus; D006801:Humans; D008099:Liver / Q000166:cytology*; D009952:Ornithine / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D014168:Transferrin / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D012333:RNA, Messenger; D014168:Transferrin; D009952:Ornithine