[Transplant-induced recovery from 6-OHDA lesions of the nigro-striatal dopamine neurons in mice].
Attempts to reconstruct the damaged nigrostriatal pathway in experimental models of Parkinson disease have thus far been carried out in animals with neurotoxically induced dopamine deficiency. Our study established that unilateral 6-hydroxydopamine (6-OHDA) lesions of the nigrostriatal-dopamine (DA) neurons produced a well-characterized functional asymmetry in the behavior of C57BL/6 (H-2b) mice. The intraperitoneal administration of methamphetamine induced ipsilateral rotation at 7-20 turns/min. 11 x 10(6) syngenic DA-rich cells of embryonic ventral mesencephalon were stereotaxically transplanted in the caudate-putamen. A complete recovery of methamphetamine-induced rotational response was produced around the 60-th day after the syngenic cell suspension graft. And a complete compensation of the rotational response was also brought about with the DA-rich cells from embryonic ventral mesencephalon (crown-rump length; 10-13 mm) of allogenic C 3 H/HeN (H-2k) mice. The FACS IV analysis revealed no H-2 (Kk and Iak) antigens before transplantation of these embryonic cells. Immunohistochemistry showed that the dopaminergic fibers had grown predominantly into the ipsilateral caudate-putamen. These results provide evidence of integration of syngenic and allogenic grafts and host tissue. And the immunological response in the transplanted brain are under investigation.
Shimizu
K
K
Department of Neurosurgery, Osaka University Medical School, Japan.
Tsuda
N
N
Matsui
Y
Y
Okamoto
Y
Y
Miyao
Y
Y
Yamada
M
M
Ikeda
T
T
Hatanaka
H
H
Mogami
H
H
D000818:Animals; D004195:Disease Models, Animal; D004298:Dopamine / Q000502:physiology; D005260:Female; D006892:Hydroxydopamines; D008694:Methamphetamine / Q000494:pharmacology; D051379:Mice; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000473:pathology; D010302:Parkinson Disease, Secondary / Q000139:chemically induced / Q000601:surgery*; D013378:Substantia Nigra / Q000637:transplantation*; D014184:Transplantation, Homologous
D006892:Hydroxydopamines; D008694:Methamphetamine; D004298:Dopamine