[Immunocytochemical study of the distribution of cytoskeletal proteins in cultured neurons of the spinal cord of mouse embryos].
The distribution of tubulin, actin and neurofilamentous protein (molecular weight 160 kD) in the spinal cord neurons of mice embryos cultivated in monolayer was studied by means of monoclonal FITC- and rhodamine labeled antibodies. It was found that nerve cell development induced migration of tubulin from the neuronal soma to its processes. The tubulin filling in different processes was uneven at certain stages of cell differentiation. The actin content in the neuron was negligible. Its point concentration was observed along the neuronal processes in the vicinity of focal contacts with the lining cells. Main content of neurofilamentous protein is concentrated in the neuronal body and proximal parts of neurites. The results obtained present the pattern of distribution of cytoskeletal proteins in the spinal cord neurons.
Skibo
G G
GG
Berezovskaia
O L
OL
Koval'
L M
LM
D000199:Actins / Q000032:analysis; D000818:Animals; D002478:Cells, Cultured; D003598:Cytoskeletal Proteins / Q000032:analysis*; D007150:Immunohistochemistry; D051379:Mice; D009474:Neurons / Q000032:analysis*; D013116:Spinal Cord / Q000032:analysis* / Q000166:cytology / Q000196:embryology; D014404:Tubulin / Q000032:analysis
D000199:Actins; D003598:Cytoskeletal Proteins; D014404:Tubulin
Immunotsitokhimicheskoe issledovanie raspredeleniia tsitoskeletnykh belkov v kul'tiviruemykh neĭronakh spinnogo mozga myshinykh émbrionov.