title: The expression of insulin-like growth factor I and insulin-like growth factor II genes in the human fetal and adult brain and in glioma.
abstract: Expression of insulin-like growth factor I (IGF-I) and insulin-like growth factor II (IGF-II) genes in the human brain and in glioma was studied by slot blot and Northern analysis following hybridization of IGF-I and IGF-II cDNA probes to isolated mRNA. In the fetal brain there were major IGF-I transcripts of 7.5 and 4.4 kilobases (kb) and one major IGF-II transcript of 6.0 kb. IGF-II mRNA was less abundant in the adult brain, with IGF-II mRNA being scarcely detectable. All glioma tissue examined displayed a marked enhancement of both IGF-I and IGF-II gene expression when compared to normal brain. IGF-I transcripts of 4.4 and 2.2-1.8 kb were identified. Similar to fetal brain a major IGF-II transcript of 6.0 kb was found in the glioblastoma examined.
authors.affiliation: Karolinska Institute's Department of Psychiatry, St. Göran's Hospital, Stockholm, Sweden.
authors.forename: A C
authors.initials: AC
authors.lastname: Sandberg
authors.forename: C
authors.initials: C
authors.lastname: Engberg
authors.forename: M
authors.initials: M
authors.lastname: Lake
authors.forename: H
authors.initials: H
authors.lastname: von Holst
authors.forename: V R
authors.initials: VR
authors.lastname: Sara
mesh_terms: D001921:Brain / Q000196:embryology / Q000378:metabolism*; D001932:Brain Neoplasms / Q000378:metabolism*; D005786:Gene Expression Regulation; D005865:Gestational Age; D005910:Glioma / Q000378:metabolism*; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000235:genetics* / Q000378:metabolism; D007335:Insulin-Like Growth Factor II / Q000235:genetics* / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000378:metabolism; D013002:Somatomedins / Q000235:genetics*
chemical_list: D012333:RNA, Messenger; D013002:Somatomedins; D007334:Insulin-Like Growth Factor I; D007335:Insulin-Like Growth Factor II