Heparin-like tubings. III. Kinetics and mechanism of thrombin, antithrombin III and thrombin-antithrombin complex adsorption under controlled-flow conditions.
In previous papers, we described treated tubular materials which exhibit an heparin-like antithrombic activity under dynamic conditions. In order to ascertain the heparin-like mechanism of this activity, we have studied the interactions of thrombin, antithrombin III and thrombin-antithrombin III complex with the inner face of these treated tubings under controlled-flow conditions. Moreover, the kinetics of the adsorption of thrombin were studied at different flow rates to establish the rate-determining step.
Migonney
V
V
Laboratoire de Recherches sur les Macromolécules, CSP, Université Paris-Nord, Villetaneuse, France.
Fougnot
C
C
Jozefowicz
M
M
D000327:Adsorption; D000990:Antithrombin III / Q000378:metabolism*; D001672:Biocompatible Materials; D006493:Heparin; D006801:Humans; D007700:Kinetics; D046911:Macromolecular Substances; D008962:Models, Theoretical; D011095:Polyethylenes; D013917:Thrombin / Q000378:metabolism*
D001672:Biocompatible Materials; D046911:Macromolecular Substances; D011095:Polyethylenes; D000990:Antithrombin III; D006493:Heparin; D013917:Thrombin