Cachectin/TNF stimulation of ATP synthesis in 30A-5 preadipocytes.
Cachectin/tumor necrosis factor (TNF) one of the cytokines secreted by reticuloendothelial cells and lymphocytes, evokes a multitude of biological effects in different cells. Although the effects of TNF on adipocytes generally appear rather slowly, we have found that TNF almost instantaneously stimulates respiration and respiration-coupled ATP synthesis in 30A-5 preadipocytes. This novel TNF effect might be one of the essential early steps in the cachectic mobilization of energy reserves associated with TNF action in vivo.
Lee
K H
KH
Department of Biochemistry, Purdue University, West Lafayette, Indiana 47907.
Kim
K H
KH
D019297:2,4-Dinitrophenol; D000255:Adenosine Triphosphate / Q000096:biosynthesis*; D000273:Adipose Tissue / Q000378:metabolism*; D001149:Arsenates / Q000494:pharmacology; D002460:Cell Line; D004140:Dinitrophenols / Q000494:pharmacology; D007700:Kinetics; D010085:Oxidative Phosphorylation / Q000187:drug effects; D010101:Oxygen Consumption; D010710:Phosphates / Q000378:metabolism; D010750:Phosphoproteins / Q000378:metabolism; D010766:Phosphorylation; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D001149:Arsenates; D004140:Dinitrophenols; D010710:Phosphates; D010750:Phosphoproteins; D014409:Tumor Necrosis Factor-alpha; D000255:Adenosine Triphosphate; C025657:arsenic acid; D019297:2,4-Dinitrophenol