Enhancer-independent mutants of the Cin recombinase have a relaxed topological specificity.
Cin is a member of the hin family of complementing site-specific recombinases which regulate the alternate expression of genes by inverting DNA segments. Common characteristics of this family of recombination systems are the requirement for an enhancer-like element in cis and the specificity for inversely oriented recombination sites on the same DNA molecule. We have isolated two mutants of the Cin recombinase which will efficiently recombine a substrate lacking the enhancer. In addition, these mutant proteins also catalyse efficient recombination between sites in direct orientation or on different DNA molecules. Both mutations are due to single amino acid substitutions at different positions in the protein and the two mutants have slightly different phenotypes. The finding that the loss of enhancer dependence is coupled to a change in topological specificity leads us to conclude that the enhancer determines the specificity of the system for DNA inversion.
Haffter
P
P
Department of Microbiology, Basel University, Switzerland.
Bickle
T A
TA
D001435:Bacteriophages / Q000235:genetics*; D002872:Chromosome Deletion; D004252:DNA Mutational Analysis; D004254:DNA Nucleotidyltransferases / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics; D004742:Enhancer Elements, Genetic; D010641:Phenotype; D010957:Plasmids; D011995:Recombination, Genetic
D004268:DNA-Binding Proteins; D004254:DNA Nucleotidyltransferases; C039930:cin recombinase