title: Legionella pneumophila: initial detection through evaluation of Giemsa-stained smears of a bronchial aspirate.
abstract: The presence of Legionella species in a bronchial aspirate was strongly suspected by the visualization on Giemsa-stained smears of slender violaceous intra- and extracellular bacilli despite the absence of fluorescence on smears stained with L. pneumophila serogroup 1 conjugate. Subsequently, cultures of the aspirate grew L. pneumophila reactive with serogroup 6 fluorescein-conjugated antibody.
authors.affiliation: Department of Microbiology, Mount Sinai Hospital, New York, N.Y. 10029-6574.
authors.forename: M
authors.initials: M
authors.lastname: Reitano
authors.forename: H D
authors.initials: HD
authors.lastname: Engler
authors.forename: E J
authors.initials: EJ
authors.lastname: Bottone
mesh_terms: D003581:Cytodiagnosis; D006801:Humans; D007875:Legionella / Q000302:isolation & purification*; D007876:Legionellosis / Q000175:diagnosis / Q000473:pathology; D008168:Lung / Q000382:microbiology* / Q000473:pathology