[Biochemical and immunocytochemical study of the axonal domain of mature and immature microtubules].
The axonal domain of microtubules was analyzed using biochemistry and immunocytochemistry. High resolution isofocusing was used to study the cold-labile and cold-stable fractions of various samples: thalamus, optic nerve, sciatic nerve, brachial plexus and trigeminal nerve. The cold-labile fraction from the thalamus, i.e. from a central nervous system cell population contains 20 well-resolved isotypes as the axonal fraction from the central nervous system. The cold-stable fraction from the peripheral nervous system contains only 18 isotypes. All the cold-stable fractions are characterized by an important relative quantity of isotypes 7-9, with specific patterns tissue dependent. The cold-labile fractions are specific in both the central and peripheral nervous system. Immunocytochemistry using anti-tubulin and anti-MAP2 specific antibodies was used in PEG semi-thin sections of the embryonic tectal plate at stage E10. In the axonal profiles, cold-stable fragments of microtubules were observed. The perikaryon of the young neurons, both migrating and post-migratory, contains MAP2 as the cold-labile fractions of microtubules of the axonal process. These results suggest that; tubulin is involved in the cold-stability of the axonal domain, but this role seems tissue dependent; MAP2 is a marker of neuronal differentiation; in the growing axonal process, cold-stable fragments are present and the cold-labile pool of microtubules contain a transiently expressed protein, MAP2.
Binet
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S
Faculté de Médecine, Laboratoire d'Anatomie, Paris.
Meininger
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V
D000818:Animals; D001369:Axons / Q000032:analysis*; D001917:Brachial Plexus / Q000032:analysis*; D004622:Embryo, Mammalian / Q000032:analysis; D051379:Mice; D008870:Microtubules / Q000032:analysis*; D009900:Optic Nerve / Q000032:analysis*; D012584:Sciatic Nerve / Q000032:analysis*; D013788:Thalamus / Q000032:analysis*; D014276:Trigeminal Nerve / Q000032:analysis*
Etude biochimique et immunocytochimique du domaine axonal des microtubules matures et immatures.