Reduction of 3 alpha-hydroxy-5 beta-chol-6-en-24-oic acid to lithocholic acid in rats.
After [24-14C]delta 6-lithocholic acid was injected into the cecum of rats, [14C]lithocholic acid was identified as a metabolite in feces. When the labeled delta 6-bile acid was injected intraperitoneally into bile-fistula rats, radioactivity excreted in bile was contained most abundantly in the taurine-conjugated fraction of bile acids. In the fraction, taurine conjugate of [14C]delta 6-lithocholic acid but of neither [14C]lithocholic acid nor other bile acids was found. The results showed that [24-14C]delta 6-lithocholic acid was reduced to [14C]lithocholic acid by the intestinal flora but not by the liver, which, however, was capable of conjugating delta 6-lithocholic acid with taurine.
Kimura
K
K
Department of Biochemistry, Tottori University School of Medicine, Japan.
Ogura
M
M
D000818:Animals; D001647:Bile Acids and Salts / Q000032:analysis; D005243:Feces / Q000032:analysis; D006868:Hydrolysis; D008095:Lithocholic Acid / Q000031:analogs & derivatives* / Q000096:biosynthesis* / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D010084:Oxidation-Reduction; D051381:Rats; D011919:Rats, Inbred Strains; D012588:Scintillation Counting; D013055:Spectrophotometry, Infrared
D001647:Bile Acids and Salts; C046457:3-hydroxy-6-cholen-24-oic acid; D008095:Lithocholic Acid