Method of genomic DNA cloning by the combination of cosmid shuttle vector and monoclonal antibody.
We report here the strategy to isolate the DNA fragment of any species origin which encodes cell surface antigen by using cosmid library transfection and cell sortings with a monoclonal antibody. We took the mouse melanoma antigen defined by monoclonal antibody as a model system and rescued the genomic DNA by in vitro packaging, showing the feasibility of this procedure.
Tagawa
M
M
Department of Immunology, School of Medicine, Chiba University.
Ito
T
T
Kanno
M
M
Okitsu
A
A
Sakamoto
T
T
Imai
K
K
Kuwabara
I
I
Koseki
H
H
Matsubara
H
H
Lau
Y F
YF
D000818:Animals; D000911:Antibodies, Monoclonal / Q000235:genetics*; D000954:Antigens, Surface / Q000235:genetics; D002478:Cells, Cultured; D003001:Cloning, Molecular / Q000379:methods*; D003360:Cosmids; D004247:DNA / Q000235:genetics*; D006801:Humans; D008546:Melanoma, Experimental / Q000235:genetics; D051379:Mice; D014162:Transfection
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D004247:DNA