title: Molecular heterogeneity of the isolated surface glycoprotein from variant AnTat 1.1 of Trypanosoma brucei brucei.
abstract: Variant surface glycoprotein (VSG) of Trypanosoma brucei brucei AnTat 1.1 was released by means of the procedure described by Baltz et al. ([1976], Ann. Immunol. [Inst. Pasteur] 127C, 761-774). The concanavalin-A chromatography yielded 3 VSG fractions according to the addition, in the elution buffer, of alpha-methyl-D-mannopyranoside, beta-mercaptoethanol, and sodium dodecyl sulfate. These VSG fractions showed heterogeneous behaviour on reverse-phase high performance liquid chromatography. The 3 VSG fractions as well as the myristylated VSG of AnTat 1.1 essentially consist of dimer VSG forms linked through a disulfide bridge, as judged by sodium dodecyl sulfate polyacrylamide gel electrophoresis, under reducing and nonreducing conditions.
authors.affiliation: Unité INSERM No. 16, Lille, France.
authors.forename: F
authors.initials: F
authors.lastname: Boutignon
authors.forename: M
authors.initials: M
authors.lastname: Hublart
authors.forename: V
authors.initials: V
authors.lastname: Gomes
authors.forename: L
authors.initials: L
authors.lastname: Mendonca-Previato
authors.forename: D
authors.initials: D
authors.lastname: Tetaert
authors.forename: G
authors.initials: G
authors.lastname: Huet-Duvillier
authors.forename: P
authors.initials: P
authors.lastname: Degand
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D015151:Immunoblotting; D008562:Membrane Glycoproteins / Q000032:analysis*; D009227:Myristic Acids; D051381:Rats; D014346:Trypanosoma brucei brucei / Q000032:analysis*
chemical_list: D008562:Membrane Glycoproteins; D009227:Myristic Acids