Induction of unscheduled DNA synthesis and micronucleus formation in Syrian hamster embryo fibroblasts treated with cysteine S-conjugates of chlorinated hydrocarbons.
S-(chloroethyl)-cysteine (CEC) and S-(1,2-dichlorovinyl)-cysteine (DCVC) have been proposed as intermediates in the metabolic transformation of the carcinogens 1,2-dichloroethane and 1,1,2-trichloroethylene. We have tested the ability of CEC and DCVC to induce DNA repair and genotoxic effects at the chromosomal level by comparative assessment of unscheduled DNA synthesis induction and micronucleus formation in Syrian hamster embryo fibroblasts. CEC induced a potent and dose-dependent response in both assays, whereas DCVC treatment resulted in a comparatively weak induction of DNA repair and failed to raise micronucleus formation above control rates. Inhibition of cysteine conjugate beta-lyase diminished the effect of DCVC, but had no influence on the genotoxicity of CEC either in the unscheduled DNA synthesis or micronucleus assay.
Vamvakas
S
S
Institut für Toxikologie, Universität Würzburg, Federal Republic of Germany.
Dekant
W
W
Schiffmann
D
D
Henschler
D
D
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D006224:Cricetinae; D003545:Cysteine / Q000031:analogs & derivatives* / Q000633:toxicity; D004260:DNA Repair; D005347:Fibroblasts / Q000187:drug effects; D006843:Hydrocarbons, Chlorinated / Q000633:toxicity*; D008647:Mesocricetus; D015162:Micronucleus Tests
D006843:Hydrocarbons, Chlorinated; C045285:S-(2-chloroethyl)cysteine; C039961:S-(1,2-dichlorovinyl)cysteine; D003545:Cysteine