Direct and short-term culture preparation of chorionic villi. Is any one method best?
Chorionic villi from 20 diagnostic cases were prepared for cytogenetic analysis by a direct and two short-term culture methods. No significant differences were found between the methods in the quality and quantity of metaphases obtained. A further study using villi from 20 pre-termination patients indicated an inherent variation in the quality of villi resulting in inconsistent processing and variation in the response of a sample to the methods. This suggests that it would be advantageous to process the villi by more than one method.
Holmes
D S
DS
Department of Clinical Genetics, Birmingham Maternity Hospital, Edgbaston, U.K.
Fifer
A M
AM
Mackenzie
W E
WE
Griffiths
M J
MJ
Newton
J R
JR
D002478:Cells, Cultured; D015193:Chorionic Villi Sampling / Q000379:methods*; D005260:Female; D006801:Humans; D008677:Metaphase; D008722:Methods; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D013997:Time Factors