Species differences in hepatic microsomal drug-metabolizing enzymes.
The activity of some metabolizing enzymes was assessed in the liver microsomes of Acomys cahirinus, mice and rats. The enzymatic studies were followed by the determination of cerebral level of apomorphine (APO), imipramine (IMI) and its metabolite desipramine (DMI) of animals treated with a single dose of APO or IMI. It was found that the level of cytochrome P-450 and the activity of IMI demethylase and glucuronyltransferase in the liver microsomes of rats was significantly higher than those in the liver microsomes of Acomys and mice. The brain levels of APO, IMI and DMI were different in investigated species and IMI and DMI levels in the brain of Acomys, mice and rats corresponded to the activity of IMI demethylase in the liver microsomes of these species.
Danek
L
L
Institute of Pharmacology, Polish Academy of Sciences, Kraków.
Nocoń
H
H
Tarnawska
A
A
Melzacka
M
M
D000818:Animals; D001058:Apomorphine / Q000378:metabolism; D001921:Brain / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System; D003891:Desipramine / Q000378:metabolism; D007099:Imipramine / Q000378:metabolism; D008297:Male; D051379:Mice; D008862:Microsomes, Liver / Q000378:metabolism*; D009115:Muridae; D010089:Oxidoreductases, N-Demethylating / Q000378:metabolism; D004364:Pharmaceutical Preparations / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013045:Species Specificity
D004364:Pharmaceutical Preparations; D003577:Cytochrome P-450 Enzyme System; D010089:Oxidoreductases, N-Demethylating; C058551:imipramine demethylase; D001058:Apomorphine; D007099:Imipramine; D003891:Desipramine