Preparation and characterization of a reconstituted stratum corneum film as a model membrane for skin transport.
A zwitterionic surfactant, 6-eicosyldimethyl ammoniohexanoate (C20AH), completely disaggregates stratum corneum into individual cells. The cells can be cast into a film of reconstituted stratum corneum (RSC). Such films prepared from pig and human skin mimic intact human stratum corneum in microscopic, mechanical, and barrier evaluations. The films are useful as model membranes for skin transport experiments.
Kock
W R
WR
Procter and Gamble Company, Miami Valley Laboratories, Cincinnati, OH 45239.
Berner
B
B
Burns
J L
JL
Bissett
D L
DL
D000818:Animals; D001693:Biological Transport, Active; D002469:Cell Separation; D004548:Elasticity; D006801:Humans; D066298:In Vitro Techniques; D008566:Membranes / Q000378:metabolism / Q000648:ultrastructure; D008854:Microscopy, Electron; D008954:Models, Biological; D012867:Skin / Q000033:anatomy & histology* / Q000378:metabolism; D013552:Swine; D014867:Water / Q000378:metabolism
D014867:Water