Carcinogenicity and toxicity study of m-phenylenediamine administered in the drinking-water to (C57BL/6 x C3H/He)F1 mice.
m-Phenylenediamine (m-PDA, CAS: 108-45-2), a component of hair-dye formulations, was administered in the drinking-water to groups of female and male (C57BL/6 x C3H/He)F1 (B6C3F1) mice at concentrations of 0.02 or 0.04% for 78 wk. All the surviving mice were killed after a further 5-7 wk on untreated drinking-water, 83-85 wk after the start of treatment. Survival of the treated mice was similar to that of the corresponding controls. Body weights were significantly lower in high-dose females and males and somewhat lower in low-dose females than in the controls. The incidences of hepatocellular tumours were low to moderate in all male groups and in the control females, but the treated groups had significantly lower incidences than the controls. A few tumours of the lungs, haematopoietic organs and other organs and tissues were observed in all female and male groups. However, there were no statistically significant increases in the incidences of tumours in these organs and tissues in m-PDA-treated mice of either sex. Under the conditions of this study m-PDA showed no carcinogenic potential in either female or male B6C3F1 mice when administered in drinking-water. No non-neoplastic changes attributable to the compound were found in the treated mice, except for the deposition of brown pigment in follicular epithelial cells of the thyroid gland and in macrophages in some organs and tissues, and pigment impregnation of the bronchioli.
Amo
H
H
Aichi Women's College, Nagoya, Japan.
Matsuyama
M
M
Amano
H
H
Yamada
C
C
Kawai
M
M
Miyata
N
N
Nakadate
M
M
D000818:Animals; D001835:Body Weight / Q000187:drug effects; D004326:Drinking; D005260:Female; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D009374:Neoplasms, Experimental / Q000139:chemically induced*; D010655:Phenylenediamines / Q000633:toxicity*
D010655:Phenylenediamines; C008381:3-phenylenediamine