Zonae pellucidae of salt-stored hamster and human eggs: their penetrability by homologous and heterologous spermatozoa.
Zonae pellucidae of unfertilized hamster and human eggs remain penetrable by homologous spermatozoa when they are stored in highly concentrated salt solutions [Yanagimachi et al., 1979]. Neutralization of salt solutions with the appropriate pH buffers is recommended for long-term storage of the eggs (zonae), because zonae may gradually swell or even dissolve in acidic or alkaline solutions. At least for the hamster, 2 M ammonium sulfate solution containing 40 mM Hepes and 0.5% dextran (pH 7.0) seems to serve the purpose satisfactorily. Thorough rinsing of salt-stored zonae prior to insemination is important, but inclusion of protease inhibitors in the rinsing medium does not appear to be essential. A dialysis method is recommended when a large number of salt-stored eggs must be rinsed at one time. Salt-stored hamster zonae, like fresh (control) zonae, permit attachment and penetration of hamster spermatozoa, but not human spermatozoa. Salt-stored human zonae, on the other hand, allow penetration by not only human spermatozoa, but also hamster spermatozoa.
Yoshimatsu
N
N
Department of Anatomy and Reproductive Biology, University of Hawaii School of Medicine, Honolulu 96822.
Yanagimachi
R
R
Lopata
A
A
D000645:Ammonium Sulfate / Q000494:pharmacology; D000818:Animals; D006224:Cricetinae; D005306:Fertilization; D006801:Humans; D007314:Insemination; D008297:Male; D010063:Ovum / Q000502:physiology*; D011309:Preservation, Biological; D012492:Salts; D013084:Sperm-Ovum Interactions / Q000187:drug effects; D015044:Zona Pellucida / Q000502:physiology*
D012492:Salts; D000645:Ammonium Sulfate