Accurate and specific HPLC assay of coproporphyrinogen III oxidase activity in human peripheral leucocytes.
An HPLC method is described for the assay of coproporphyrinogen III oxidase in human leucocytes. The optimal pH for the assay was 6.5-7.0 and the Km for coproporphyrinogen III was 0.12 +/- 0.021 mumol/l. The mean activity in 28 apparently health subjects was 0.249 (2 SD range 0.130-0.368) nmol/h per mg protein. In two patients with hereditary coproporphyria, the activities were 0.029 and 0.078 nmol/h per mg protein.
Guo
R
R
Division of Clinical Cell Biology, MRC Clinical Research Centre, Harrow, Middlesex, UK.
Lim
C K
CK
Peters
T J
TJ
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002851:Chromatography, High Pressure Liquid; D003304:Coproporphyrinogen Oxidase / Q000097:blood*; D006801:Humans; D007962:Leukocytes / Q000201:enzymology*; D008875:Middle Aged; D010088:Oxidoreductases / Q000097:blood*; D012016:Reference Values
D010088:Oxidoreductases; D003304:Coproporphyrinogen Oxidase