title: pH of organ-culture-stored corneas.
abstract: Changes in intracorneal and storage-medium pH values of organ-culture-stored cat corneas were monitored over a 4-week period. The intracorneal pH was determined using the phosphorus-31 magnetic resonance spectroscopy (31P MRS) chemical shift of inorganic orthophosphate in conjunction with a standard pH titration curve. We incubated 32 adult cat corneas using two similar standard organ-culture methods, one with chondroitin sulfate (method 1) and the other without (method 2). Time-course data at 0, 1, 3 and 4 weeks of storage were used to calculate the rate of pH change. The intracorneal pH was not changed significantly for either organ-culture method; however, the storage-medium pH rate of change declined significantly for both methods (method 1, 0.15 pH units/week; method 2, 0.12 pH units/week). The difference between intracorneal and storage-medium pH values over time increased at a rate of 0.12 and 0.11 pH units/week for method 1 and method 2, respectively. The declining storage-medium pH in conjunction with the maintenance of intracorneal pH contributes to an increased metabolic demand on the cornea.
authors.affiliation: Division of Ophthalmology, Case Western Reserve University, Cleveland.
authors.forename: J H
authors.initials: JH
authors.lastname: Lass
authors.forename: J V
authors.initials: JV
authors.lastname: Greiner
authors.forename: P
authors.initials: P
authors.lastname: Meneses
authors.forename: D C
authors.initials: DC
authors.lastname: Morgan
authors.forename: S K
authors.initials: SK
authors.lastname: Medcalf
authors.forename: D M
authors.initials: DM
authors.lastname: Collie
authors.forename: D L
authors.initials: DL
authors.lastname: Skelnik
authors.forename: T
authors.initials: T
authors.lastname: Glonek
mesh_terms: D000818:Animals; D002415:Cats; D003315:Cornea / Q000502:physiology*; D003470:Culture Media; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D009924:Organ Culture Techniques
chemical_list: D003470:Culture Media