Biochemical characterization of the pancreatic cholecystokinin receptor: a possible marker of cell differentiation and development.
The biochemical expression of the cholecystokinin (CCK) receptor on the surface of the pancreatic acinar cell is a potential marker of the state of differentiation of that cell. In this report we review the basis for and the results of the use of affinity labeling techniques for the biochemical characterization of this macromolecular receptor assembly on the adult rat pancreatic acinar cell. A series of specially designed molecular probes are used to define the subunit structure of this receptor, based on the relationships between the sites of covalent attachment of these probes and their receptor-binding domains. We suggest that the receptor-binding domain resides on a Mr = 85,000-95,000 subunit, whereas a distinct Mr = 80,000 also exists as part of this complex.
Miller
L J
LJ
Gastroenterology Unit, May Clinic, Rochester, Minnesota 55905.
Powers
S P
SP
D000345:Affinity Labels; D000818:Animals; D002454:Cell Differentiation; D002766:Cholecystokinin / Q000502:physiology*; D015335:Molecular Probes; D010179:Pancreas / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D011949:Receptors, Cholecystokinin / Q000378:metabolism*
D000345:Affinity Labels; D015335:Molecular Probes; D011949:Receptors, Cholecystokinin; D002766:Cholecystokinin