Pentylenetetrazole inhibits glutamate dehydrogenase and aspartate aminotransferase, and stimulates GABA aminotransferase in homogenates from rat cerebral cortex.
The mechanism by which pentylenetetrazole provokes convulsions in animals has been investigated by measuring its influence in vitro on the activities of several enzymes of glutamate metabolism in rat brain homogenates. Pentylenetetrazole does not affect the specific activities of glutamine synthetase, glutaminase, or glutamate decarboxylase; it inhibits those of glutamate dehydrogenase and aspartate aminotransferase, and stimulates that of gamma-aminobutyric acid (GABA) aminotransferase. The overall consequence of the action of pentylenetetrazole on the activities of these enzymes should be an increase in the concentration of glutamate and a decrease in that of GABA. This modulation of glutamate and GABA metabolism by pentylenetetrazole could contribute to the triggering of convulsions.
Lacoste
L
L
Département de biochimie, Faculté de médecine, Université Laval, Québec, Canada.
Bartolucci
S
S
Lapointe
J
J
D000612:4-Aminobutyrate Transaminase / Q000378:metabolism*; D000818:Animals; D001219:Aspartate Aminotransferases / Q000037:antagonists & inhibitors*; D002540:Cerebral Cortex / Q000187:drug effects / Q000201:enzymology*; D005969:Glutamate Dehydrogenase / Q000037:antagonists & inhibitors*; D066298:In Vitro Techniques; D010433:Pentylenetetrazole / Q000494:pharmacology*; D051381:Rats
D005969:Glutamate Dehydrogenase; D001219:Aspartate Aminotransferases; D000612:4-Aminobutyrate Transaminase; D010433:Pentylenetetrazole