Cytochemical study of the Golgi apparatus and related organelles of the secretory ameloblasts of rat molar tooth germs cultured with and without colchicine.
The Golgi apparatus and Golgi-associated endoplasmic reticulum lysosome (GERL) were examined in the ameloblasts with a cytochemical marker, osmium impregnation, and two enzyme markers, thiamine pyrophosphatase (TPPase) and acid phosphatase (ACPase). In control cultured germs, osmium deposit appeared in one to two immature side cisternae of Golgi stacks; TPPase activity was restricted in a few mature side cisternae and condensing vacuoles. ACPase activity existed in the GERL and, sometimes, in the mature side-cisternae and condensing vacuoles. These findings show that Golgi stacks of ameloblasts consist of several distinct compartments. In colchicine-treated tooth germs, there were morphological and cytochemical changes in both Golgi stacks and GERL. The Golgi apparatus was fragmented and its stacks were scattered throughout the supranuclear region. In some stacks, the number of osmium-positive cisternae was greater than normal; in others they were absent. TPPase and ACPase activity was absent or diminished. These findings suggest the importance of microtubules in the organization of Golgi complex and GERL in the secretory ameloblast.
Matsuo
S
S
First Department of Oral Anatomy, Osaka University Faculty of Dentistry, Japan.
Takano
Y
Y
Wakisaka
S
S
Ichikawa
H
H
Nishikawa
S
S
Itotagawa
T
T
Akai
M
M
D000135:Acid Phosphatase / Q000378:metabolism; D000565:Ameloblasts / Q000187:drug effects* / Q000201:enzymology; D000818:Animals; D003078:Colchicine / Q000494:pharmacology*; D004721:Endoplasmic Reticulum / Q000187:drug effects; D006056:Golgi Apparatus / Q000187:drug effects*; D006651:Histocytochemistry; D008247:Lysosomes / Q000187:drug effects; D008854:Microscopy, Electron; D008963:Molar; D015388:Organelles / Q000187:drug effects*; D051381:Rats; D011919:Rats, Inbred Strains; D013834:Thiamine Pyrophosphatase / Q000378:metabolism; D014083:Tooth Germ / Q000648:ultrastructure*
D000135:Acid Phosphatase; D013834:Thiamine Pyrophosphatase; D003078:Colchicine