Testing for the teratogenicity of pulsed ultrasound in mice.
Our replicate of a study by Takabayashi et al. (1981) Effects of pulse-wave ultrasonic irradiation on mouse embryo. Cho-Onpa Igaku (Supersonic Medicine) 8, 286-288 failed to show any effects of exposure in utero of mice to spatial average, temporal maximum intensities of 60 W/cm2. Fetuses were exposed at 8 days post fertilization and assessed at 18 days for fetal weight, resorptions, premature deaths, and malformations. Carrier frequency in the pulse was 2 MHz. Pulse lengths of 10 microseconds and pulse repetition frequencies of 1000 and 200 Hz yielded spatial average, temporal average intensities of 0.6 and 0.1 W/cm2. Total exposure time was 5 min. Our results provide no basis to conclude that conditions relevant to human fetal monitoring will cause developmental, externally visible anomalies in mice exposed as 8-day fetuses.
Child
S Z
SZ
Rochester Center for Biomedical Ultrasound, University of Rochester, NY 14627.
Carstensen
E L
EL
Gates
A H
AH
Hall
W J
WJ
D000818:Animals; D000013:Congenital Abnormalities / Q000209:etiology*; D005260:Female; D005313:Fetal Death / Q000209:etiology; D051379:Mice; D008807:Mice, Inbred BALB C; D011247:Pregnancy; D014465:Ultrasonics / Q000009:adverse effects*