A new technique for orcein banding with acid treatment.
A rapid method for banding plant chromosomes using hydrochloric acid with orcein is suggested. The technique has been successful in both dioecious and monoecious families with short chromosomes. It involves pretreatment with an oxyquinoline-paradichlorobenzene mixture, fixation in acetic ethanol and treatment with hydrochloric acid at 60 C followed by staining with orcein. Stained chromosome bands and interbands are reproducible and species specific.
Chattopadhyay
D
D
Department of Botany, University of Calcutta, India.
Sharma
A K
AK
D002871:Chromosome Banding / Q000379:methods*; D010078:Oxazines; D010944:Plants / Q000032:analysis*
D010078:Oxazines; C002313:PAcein