title: Duration of storage of cryopreserved human embryos.
abstract: The incidence of cell injury, embryo survival, and implantation following cryopreservation of zygotes and two- to five-cell embryos was studied in 100 patients in order to evaluate the effect of duration of storage. The incidence of individual cell survival was 58% regardless of the length of time kept in liquid nitrogen or the stage of the embryo at freezing. There were 104 of 208 (50%) thawed embryos that survived completely intact, and of those, 24 implanted successfully. Twenty-one (21%) patients had a clinical pregnancy; two of them miscarried Neither the survival of zygotes or cleaved embryos upon thawing nor the incidence of implantation was affected by the duration of cryostorage.
authors.affiliation: Reproductive Biology Associates, Emory University, Atlanta, Georgia.
authors.forename: J
authors.initials: J
authors.lastname: Cohen
authors.forename: K L
authors.initials: KL
authors.lastname: Inge
authors.forename: S R
authors.initials: SR
authors.lastname: Wiker
authors.forename: G
authors.initials: G
authors.lastname: Wright
authors.forename: C B
authors.initials: CB
authors.lastname: Fehilly
authors.forename: T G
authors.initials: TG
authors.lastname: Turner
mesh_terms: D002970:Cleavage Stage, Ovum / Q000502:physiology; D004624:Embryo Transfer; D004622:Embryo, Mammalian; D005260:Female; D005615:Freezing; D006801:Humans; D007986:Luteinizing Hormone / Q000378:metabolism; D010062:Ovulation Induction; D011247:Pregnancy; D011309:Preservation, Biological
chemical_list: D007986:Luteinizing Hormone