title: Preparation of human hemoglobin Ao for possible use as a blood substitute.
abstract: A procedure is presented for the preparation of a purified fraction of adult human hemoglobin (HbAo) from one unit of outdated blood. The entire process requires less than 16 h and gives a sterile, endotoxin-free solution of HbAo (approximately 30 g) in a yield of 50%. The solutions are isoionic with a conductivity of less than 15 mu mhos and less than 2 mmol total phosphorus per mol heme. The methemoglobin content is less than 1% and on storage at 4 degrees C rises less than 1% per month.
authors.affiliation: Letterman Army Institute of Research, Blood Research Division, Presidio of San Francisco, CA 94129-6800.
authors.forename: S M
authors.initials: SM
authors.lastname: Christensen
authors.forename: F
authors.initials: F
authors.lastname: Medina
authors.forename: R W
authors.initials: RW
authors.lastname: Winslow
authors.forename: S M
authors.initials: SM
authors.lastname: Snell
authors.forename: A
authors.initials: A
authors.lastname: Zegna
authors.forename: M A
authors.initials: MA
authors.lastname: Marini
mesh_terms: D000328:Adult; D001773:Blood Cells; D001802:Blood Substitutes; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D006441:Hemoglobin A / Q000302:isolation & purification*; D006461:Hemolysis; D006801:Humans; D007525:Isoelectric Focusing
chemical_list: D001802:Blood Substitutes; C028881:hemoglobin A(0); D006441:Hemoglobin A