title: Secretion of newly synthesized proteins into the extracellular fluid of the rabbit hippocampus.
abstract: Extracellular proteins were collected continuously from the hippocampus of the rabbit by slow perfusion of a protein-permeable thin tubing. After pulse labelling with radioactive amino acids via the tubing a rapid appearance of labelled proteins was seen in the extracellular fluid. The half-life of the proteins in the extracellular space varied between 15 min and 2 h. Protein fractions were separated with gel electrophoresis or HPLC. The extracellular fraction differed distinctly from intracellular proteins with respect to the labelling pattern.
authors.affiliation: Institute of Neurobiology, University of Göteborg, Sweden.
authors.forename: B
authors.initials: B
authors.lastname: Nyström
authors.forename: J O
authors.initials: JO
authors.lastname: Karlsson
authors.forename: A
authors.initials: A
authors.lastname: Hamberger
mesh_terms: D000818:Animals; D005110:Extracellular Space / Q000378:metabolism*; D006624:Hippocampus / Q000378:metabolism*; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000378:metabolism*; D011817:Rabbits; D013997:Time Factors
chemical_list: D009419:Nerve Tissue Proteins