Syntheses and properties of circular dichroism active phospholipids.
A group of circular dichroism (CD) active phospholipids has been synthesised, in which one or both acyl chains has been replaced with a cinnamoyl or azobenzene chromophore-containing acid. Studies on the structure, CD activity and thermodynamic property of liposome membranes composed of CD active phospholipids were carried out. CD active liposomes were found to be stable, normal liposomes of approximately 550 A diameter based on the electron micrograph and dynamic light scattering, and to have thermodynamic property similar to the conventional phospholipid membranes without serious perturbation by aromatic bulk groups based on DSC. Liposomes composed of phospholipid having two trans-azobenzene chromophores showed an extremely large CD enhancement even well above Tc. This CD enhancement was drastically changed by the presence of cis-azobenzene chromophore and cis-cis isomer content after irradiation was higher than the theoretical value, suggesting the importance of interchromophore interaction in the liposome membranes.
Nishiya
T
T
Artificial Cells and Organs Research Centre, McGill University, Montreal, PQ, Canada.
Okumura
Y
Y
Chang
T M
TM
D002942:Circular Dichroism; D007536:Isomerism; D008081:Liposomes; D008854:Microscopy, Electron; D010743:Phospholipids / Q000032:analysis / Q000138:chemical synthesis*; D013816:Thermodynamics
D008081:Liposomes; D010743:Phospholipids