Random locomotion and phagocytosis of monocytes in undiluted normal serum and in undiluted SLE serum.
Time-lapse filming was performed with normal monocytes in normal serum and in SLE serum, with and without yeast cell phagocytosis. Undiluted sera were used. After incubation of the monocytes with SLE serum we found a decreased velocity of random movement and a change of morphology to more condensed, rounded-up and un-spread cells with a decreased yeast cell phagocytosis. No obvious correlation was found between the SLE serum induced impairment of monocyte function and disease activity, complement levels or the presence of immune complexes. We conclude that monocyte in vitro membrane functions, such as random motility, spreading and yeast cell phagocytosis, are impaired in the presence of undiluted SLE serum.
Rydgren
L
L
Department of Rheumatology, University Hospital of Lund, Sweden.
Sturfelt
G
G
Svensson
B
B
D000328:Adult; D002465:Cell Movement; D005260:Female; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000097:blood*; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000502:physiology*; D010587:Phagocytosis; D010781:Photography