Effect of histamine on the gene expression and secretion of complement components C2, factor B and C3 in murine macrophages--an opposite signal processing via H1 and H2 receptors.
The effect of histamine on the gene expression, biosynthesis and secretion of C2, factor B and C3 was studied in mouse resident and starch elicited peritoneal macrophages. Measuring the quantity of specific mRNAs and biosynthetically labelled proteins a down-regulation of the biosynthesis of C2, factor B and C3 via H2 receptor was found. On the contrary, histamine displayed no visible H1 receptor mediated influence on the biosynthesis of C2 and factor B. However, via H1 receptor a considerably up-regulation of C3 gene expression and biosynthesis was demonstrated. The data suggest a distinct signal processing mechanism acting on H1 and H2 receptors resulting in opposite effects on the transcriptions of C3 gene in macrophages.
Falus
A
A
Division of Immunology and Molecular Biology, National Institute of Rheumatology and Physiotherapy, Budapest, Hungary.
Meretey
K
K
D000818:Animals; D015152:Blotting, Northern; D002478:Cells, Cultured; D003175:Complement C2 / Q000235:genetics / Q000378:metabolism*; D003176:Complement C3 / Q000235:genetics / Q000378:metabolism*; D011415:Complement Factor B / Q000235:genetics / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004792:Enzyme Precursors / Q000378:metabolism*; D006632:Histamine / Q000494:pharmacology*; D008264:Macrophages / Q000276:immunology*; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D011968:Receptors, Histamine / Q000502:physiology
D003175:Complement C2; D003176:Complement C3; D004792:Enzyme Precursors; D011968:Receptors, Histamine; D006632:Histamine; D011415:Complement Factor B