[Removal of surface antigens and changes in metastatic potential of transplantable rat rhabdomyosarcoma RA-2 cells].
Cells of rat rhabdomyosarcoma RA-2 selected for high metastatic potential and affinity to lung tissue were (125I)-labelled in the presence of iodogene. The spectrum of 125I-labelled cell surface antigens was investigated by SDS-PAGE. Radionuclide was incorporated by proteins (or glycoproteins) with molecular weight of 30, 36, 47, 57, 78, 90, 102, 124, 180 and 250 kDa. After incubation of cells with 0.05-0.2% triton X-100 solution all the proteins except for those with molecular weight of 47 and 57 kDa were washed off the cellular surface; metastatic potential (MP) of triton-100 treated cells was 10 times as low as that of intact cells.
Guzhova
I V
IV
Margulis
B A
BA
D000818:Animals; D000951:Antigens, Neoplasm / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D008970:Molecular Weight; D009362:Neoplasm Metastasis / Q000473:pathology*; D051381:Rats; D012208:Rhabdomyosarcoma / Q000276:immunology / Q000473:pathology*
D000951:Antigens, Neoplasm; D000954:Antigens, Surface
Udalenie poverkhnostnykh antigenov i izmenenie metastaticheskogo potentsiala kletok perevivaemoĭ rabdomiosarkomy RA-2 krys.