Effects of pentobarbital on behavioral and synaptic plasticities in crayfish.
Intra-abdominal injections of 90 mg/kg (3.5 x 10(-4) M estimated body concentration) sodium pentobarbital (PB) eliminated the cheliped closing response and eyestalk withdrawal response in crayfish (Procambarus clarkii). Repeated injections produced tolerance to both of these behavioral measures within several days. At glutamatergic synapses of the opener muscle, PB at dosages from 10(-7) to 10(-4) M had no significant effect on non-facilitated transmitter release evoked by 1 Hz stimuli. Facilitated transmitter release at 10 Hz stimuli was significantly decreased by 10(-3) M PB but was not significantly affected at lower concentrations. At 10(-4) M and 10(-3) M, PB significantly reduced the ratio of excitatory postsynaptic potential (EPSP) amplitudes at 10 Hz to those at 1 Hz. The frequency of spontaneous miniature EPSPs (MEPSPs) was reduced by 10(-4) M PB to about half of the control level, while MEPSP amplitudes and time constants were not significantly affected. These results suggest that the ability of PB to depress various crayfish behaviors is due, at least in part, to a presynaptic ability of the drug to depress facilitation of transmitter release at glutamatergic synapses.
Winegar
B D
BD
Department of Zoology, University of Texas, Austin 78712.
Bittner
G D
GD
Leslie
S W
SW
D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D003400:Astacoidea / Q000502:physiology*; D001522:Behavior, Animal / Q000187:drug effects*; D004558:Electric Stimulation; D066298:In Vitro Techniques; D009469:Neuromuscular Junction / Q000187:drug effects / Q000502:physiology*; D009473:Neuronal Plasticity / Q000187:drug effects*; D010424:Pentobarbital / Q000494:pharmacology*; D012018:Reflex / Q000187:drug effects
D010424:Pentobarbital