Reversible prevention of platelet activation by (E,Z)-4,5,9-trithiadodeca-1,6,11-triene 9-oxide (ajoene) in dogs under extracorporeal circulation.
Ajoene ((E,Z)-4,5,9-trithiadodeca-1,6,11-triene 9-oxide), an organosulfur compound derived from garlic inhibits platelet activation induced ex vivo by all known agonists. The effect of ajoene on the thrombocytopenia associated with the circulation of blood through extracorporeal devices such as dialyzers or oxygenators was studied under the following conditions: Ex vivo using fresh, heparinized human blood, circulating through a dialyzer or oxygenator, and in vivo, in dogs subjected to extracorporeal circulation. In both experimental conditions ajoene proved very efficacious in preventing platelet loss (60-65% loss in controls vs. 15-20% loss in the presence of ajoene, p less than 0.01). Moreover, recuperation of platelet function was achieved after 3-4 h in the in vivo experiments.
Apitz-Castro
R
R
Laboratory of Thrombosis Experimental, Centro de Biofisica y Bioquimica, IVIC, Caracas, Venezuela.
Ledezma
E
E
Escalante
J
J
Jorquera
A
A
Pinãte
F M
FM
Moreno-Rea
J
J
Carrillo
G
G
Leal
O
O
Jain
M K
MK
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000818:Animals; D002315:Cardiopulmonary Bypass; D003094:Collagen / Q000494:pharmacology; D003956:Dialysis; D004220:Disulfides / Q000008:administration & dosage / Q000494:pharmacology*; D004285:Dogs; D005112:Extracorporeal Circulation; D006439:Hemodynamics / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D007275:Injections, Intravenous; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology*; D010975:Platelet Aggregation Inhibitors; D010976:Platelet Count; D013454:Sulfoxides; D013921:Thrombocytopenia / Q000139:chemically induced; D013997:Time Factors
D004220:Disulfides; D010936:Plant Extracts; D010975:Platelet Aggregation Inhibitors; D013454:Sulfoxides; D000244:Adenosine Diphosphate; D003094:Collagen; C048980:ajoene