Biochemical method for molecular weight determinations of proteins and other macromolecules.
The invertase of Ricinus communis complexes with proteins, polyvinylpyrrolidone, polyethylene glycol, heparin and dextran sulfate. This association produces an increase of invertase activity. The minimal concentration of activator giving the maximal activation was attained at the same molarity for a given amount of enzyme for all macromolecules studied. These conditions are used for the molecular weight determination of the activating substance. The method may be used for the molecular weight determination of polymeric substances with a molecular weight in the range from 5000 to 1000,000 Da.
Sampietro
A R
AR
Cátedra de Fitoquímica, Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, Argentina.
Prado
F E
FE
Sayago
J E
JE
Vattuone
M A
MA
D004789:Enzyme Activation; D006026:Glycoside Hydrolases; D046911:Macromolecular Substances; D008722:Methods; D008970:Molecular Weight; D010947:Plants, Toxic; D012278:Ricinus / Q000201:enzymology; D043324:beta-Fructofuranosidase
D046911:Macromolecular Substances; D006026:Glycoside Hydrolases; D043324:beta-Fructofuranosidase