title: Platelet activating factor antagonism with BN52021 protects the kidney against acute ischemic injury.
abstract: The present study has been conducted to evaluate the eventual role of the platelet activating factor (PAF) in post-ischemic recovery of renal function, using BN52021, a PAF receptor antagonist, in the anesthetized rat. In Groups 1 and 2, animals were maintained hydropenic (fractional excretion of sodium less than 1%), while in Groups 3 and 4, studies were performed under extracellular expansion (0.9% NaCl, 5% of body weight), a procedure known to protect the kidney from ischemic injury. Groups 1 and 3 (control animals) were untreated, whereas Groups 2 and 4 received an intravenous bolus of BN52021 (3mg/kg), immediately before clearance studies. After three 20-minute control periods, the left renal artery was occluded during 30 minutes. Four consecutive 20-minute clearance periods were taken after the release of occlusion. In Groups 1 and 2, urine flow (UV) increased from 3.1 +/- 0.9 to 20.9 +/- 3.6 ml/min, and from 6.8 +/- 0.3 to 40.4 +/- 4.5 ml/min, respectively, from control to the last period of recovery. Glomerular filtration (GFR) recovered to 38% of control values, from 0.58 +/- 0.09 to 0.22 +/- 0.10 ml/min in Group 1, as opposed to Group 2, where GFR recovered to 69%, from 0.61 +/- 0.07 to 0.42 +/- 0.06 ml/min. Urinary sodium (UNaV) increased from 0.3 +/- 0.1 to 3.0 +/- 0.9 microEq/min, and from 1.0 +/- 0.3 to 6.0 +/- 0.7 microEq/min, in Groups 1 and 3. UV rose from 14.3 +/- 2.5 to 35.3 +/- 3.5 microliters/min, and from 14.2 +/- 2.1 to 68.6 +/- 9.3 microliters/min, in Groups 3 and 4, during the same periods.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Physiology, University of Sherbrooke, Canada.
authors.forename: G E
authors.initials: GE
authors.lastname: Plante
authors.forename: P
authors.initials: P
authors.lastname: Sirois
authors.forename: P
authors.initials: P
authors.lastname: Braquet
mesh_terms: D058186:Acute Kidney Injury / Q000517:prevention & control*; D000818:Animals; D004224:Diterpenes; D005260:Female; D046934:Ginkgolides; D007783:Lactones / Q000494:pharmacology*; D010972:Platelet Activating Factor / Q000037:antagonists & inhibitors*; D051381:Rats
chemical_list: D004224:Diterpenes; D046934:Ginkgolides; D007783:Lactones; D010972:Platelet Activating Factor; C045856:ginkgolide B