Rat hepatic microsomal cytochrome b5. A simple large-scale purification procedure and antibody production by antigen-containing liposomes.
Cytochrome b5 from rat liver microsomes (microsomal fractions) was purified in its native form. The procedure described has great capacity, is fast, and the final product is pure as judged from SDS/polyacrylamide-gel electrophoresis. Antibodies to cytochrome b5 are obtained after administration of the antigen inserted into small unilamellar lipid vesicles.
Carlsen
J
J
Department of Biochemistry C, Panum Institute, University of Copenhagen, Denmark.
Christiansen
K
K
Jensen
H M
HM
D000818:Animals; D000917:Antibody Formation; D000941:Antigens / Q000276:immunology; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003573:Cytochrome b Group / Q000276:immunology / Q000302:isolation & purification*; D015786:Cytochromes b5; D008081:Liposomes / Q000276:immunology*; D008862:Microsomes, Liver / Q000032:analysis*; D051381:Rats
D000941:Antigens; D003573:Cytochrome b Group; D008081:Liposomes; D015786:Cytochromes b5