HPLC analysis of 6-mercaptopurine and metabolites in extracellular body fluids.
A convenient HPLC assay, which allows for the simultaneous measurement in extracellular fluids of 6-mercaptopurine and four of its metabolites, 6-thioguanine, 6-mercaptopurine riboside, 6-thioxanthine and 6-thiouric acid is described. Solid phase extraction allows for the clean isolation of analytes from plasma, urine or cerebrospinal fluid. The simultaneous determination of 6-mercaptopurine and some of its major metabolites in extracellular fluids may contribute to the monitoring of patient compliance, bioavailability, and individual variation in metabolism and absorption.
Rudy
J L
JL
Department of Pediatrics, Children's Medical Center of Dallas, Texas 75235.
Argyle
J C
JC
Winick
N
N
Van Dreal
P
P
D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D015122:Mercaptopurine / Q000031:analogs & derivatives* / Q000097:blood* / Q000134:cerebrospinal fluid / Q000652:urine; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000097:blood* / Q000134:cerebrospinal fluid / Q000188:drug therapy / Q000652:urine
D015122:Mercaptopurine