Monitoring human exposure to carcinogens by DNA adduct measurement.
Sensitive immunologic techniques are now available for the detection and quantitation of carcinogen-DNA adducts. We have developed a number of specific monoclonal antibodies which recognize DNA modified by particular carcinogens, including benzo[a]pyrene, aminopyrene, 8-methoxypsoralen plus UVA light and vinyl chloride. These antibodies can be used in competitive enzyme-linked immunosorbent assays to quantitate adducts in DNA isolated from biological samples. Samples from treated animals as well as from humans with occupational or environmental exposure to carcinogens have been studied. In addition, antibodies can be used in indirect immunohistochemical studies to localize adduct formation in various tissues or cell types.
Santella
R M
RM
Cancer Center, School of Public Health, Columbia University, New York, New York 10032.
D000911:Antibodies, Monoclonal; D001564:Benzo(a)pyrene / Q000032:analysis* / Q000633:toxicity; D015197:Carcinogenicity Tests; D002274:Carcinogens, Environmental / Q000633:toxicity*; D004247:DNA / Q000032:analysis* / Q000187:drug effects* / Q000528:radiation effects; D018736:DNA Adducts; D004784:Environmental Monitoring; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008730:Methoxsalen / Q000633:toxicity; D014466:Ultraviolet Rays
D000911:Antibodies, Monoclonal; D002274:Carcinogens, Environmental; D018736:DNA Adducts; C056612:benzo(a)pyrene-DNA adduct; D001564:Benzo(a)pyrene; D004247:DNA; D008730:Methoxsalen