Effect of dolichyl monophosphate on the permeability properties of lipid membranes.
The effect of dolichyl monophosphate on the permeability properties of dimyristoylphosphatidylcholine bilayers to alkaline cations, Ca2+ and glucose has been determined by stop-flow spectrophotometry. The results show that, in contrast to free dolichol effects, the monophosphate derivative increased the permeability following a decreasing order of the permeating particle size. Phase diagrams indicate that dolichyl monophosphate is fully incorporated into the phosphatidylcholine bilayer around 0.75% weight/weight ratio. For these ratios, the permeation of ions is higher in the gel than in the liquid crystalline state.
Alonso-Romanowski
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S
Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Buenos Aires, Argentina.
Feliz
M R
MR
Belocopitow
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E
Disalvo
E A
EA
D002118:Calcium; D002412:Cations; D004134:Dimyristoylphosphatidylcholine; D004288:Dolichol Phosphates; D005947:Glucose; D007700:Kinetics; D008051:Lipid Bilayers; D008563:Membrane Lipids; D010539:Permeability; D011106:Polyisoprenyl Phosphates; D013696:Temperature; D014867:Water
D002412:Cations; D004288:Dolichol Phosphates; D008051:Lipid Bilayers; D008563:Membrane Lipids; D011106:Polyisoprenyl Phosphates; D014867:Water; C028306:dolichol monophosphate; D005947:Glucose; D002118:Calcium; D004134:Dimyristoylphosphatidylcholine