Phytohemagglutinin-stimulated leukocyte-conditioned medium induces differentiation in acute promyelocytic leukemia cells in vitro.
Six-day cultures of FCS and PHA-LCM-stimulated whole blood from a patient with acute promyelocytic leukemia (APL) were examined for differential cell count and chromosome karyotypes. It was found that both FCS and PHA-LCM could induce partial leukemic cell differentiation and maturation to macrophage in vitro, while PHA-LCM caused lymphocyte proliferation too. The absolute number of atypical blasts and neutrophils decreased in all 6-day cultures. The majority of the dividing cells (81.4%) contained the characteristic translocation for APL, t(15; 17) (q22; q11). Thus, these were members of the leukemic myeloid lineage, whereas some cells contained normal karyotypes, which could be lymphocytes.
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Department of Hygiene and Epidemiology, Albert Szent-Györgyi Medical University, Szeged, Hungary.
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D000818:Animals; D002417:Cattle; D002454:Cell Differentiation / Q000187:drug effects*; D002675:Child, Preschool; D003470:Culture Media / Q000494:pharmacology*; D005260:Female; D005312:Fetal Blood / Q000502:physiology; D006801:Humans; D007621:Karyotyping; D015473:Leukemia, Promyelocytic, Acute / Q000235:genetics / Q000473:pathology*; D007958:Leukocyte Count; D007962:Leukocytes; D010835:Phytohemagglutinins; D014407:Tumor Cells, Cultured / Q000473:pathology*
D003470:Culture Media; D010835:Phytohemagglutinins