Diurnal variation in vasopressin and oxytocin messenger RNAs in hypothalamic nuclei of the rat.
Diurnal changes in the expression of the vasopressin (VP) and oxytocin (OT) genes in the supraoptic (SON), paraventricular (PVN) and suprachiasmatic nuclei (SCN) of the rat were investigated by dot-blot and in situ hybridization of the VP and OT mRNAs. A significant diurnal variation in VP mRNA level was measured in the SCN, with highest levels around 17.00 h and lowest levels around midnight. No variations in levels of VP mRNA and OT mRNA were detected in SON and PVN. The data indicate that the regulation of the VP gene in the SCN is independent of that in the magnocellular nuclei.
Burbach
J P
JP
Rudolf Magnus Institute for Pharmacology, University of Utrecht, The Netherlands.
Liu
B
B
Voorhuis
T A
TA
Van Tol
H H
HH
D000818:Animals; D002940:Circadian Rhythm; D008297:Male; D009693:Nucleic Acid Hybridization; D009928:Organ Specificity; D010121:Oxytocin / Q000235:genetics*; D010286:Paraventricular Hypothalamic Nucleus / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values; D013493:Suprachiasmatic Nucleus / Q000378:metabolism*; D013495:Supraoptic Nucleus / Q000378:metabolism*; D014667:Vasopressins / Q000235:genetics*
D012333:RNA, Messenger; D014667:Vasopressins; D010121:Oxytocin