DNA-fluorescence of mammalian intra-nucleolar chromatin detected by confocal laser scanning microscopy (CLSM).
The complex spatial DNA distribution in the mammalian interphase nucleus was investigated in Feulgen stained thick sections through mouse trophoblast giant nuclei after Lowicryl embedding. DNA-fluorescence was visualized using confocal laser scanning microscopy. Our results show that the spatial arrangement of major interphase chromatin areas can be precisely documented, including the distribution of small intra-nucleolar chromatin zones.
Spring
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Institute of Experimental Pathology, German Cancer Research Center, Heidelberg, Federal Republic of Germany.
Trendelenburg
M F
MF
Montag
M
M
D000818:Animals; D002466:Cell Nucleolus / Q000648:ultrastructure*; D002843:Chromatin / Q000032:analysis*; D004247:DNA; D007834:Lasers; D051379:Mice; D008855:Microscopy, Electron, Scanning / Q000379:methods*; D008856:Microscopy, Fluorescence; D014327:Trophoblasts / Q000648:ultrastructure
D002843:Chromatin; D004247:DNA