title: Surface charge and hydrophobicity of Campylobacter jejuni strains in relation to adhesion to epithelial HT-29 cells.
abstract: Hydrophobicity and surface charge of clinical isolates of Campylobacter jejuni strains were investigated by aqueous two-phase partitioning (one-step and counter-current distribution), ion exchange chromatography and hydrophobic interaction chromatography. There was a good correlation between the different physico-chemical methods reflecting the same bacterial property. All strains were negatively charged and exposed a hydrophobic surface, but to a varying extent. Bacteria with a high negative surface charge and a weak hydrophobic surface adhered better to human intestinal HT-29 cells than strains with less charge and a more hydrophobic surface. Highest adhesion was shown by a strain differing from all the others in charge properties. It was also found that the tendency to aggregate was higher among the strains showing the greatest degree of adherence.
authors.affiliation: Department of Medical Microbiology, Faculty of Health Sciences, Linköping University, Sweden.
authors.forename: A
authors.initials: A
authors.lastname: Walan
authors.forename: E
authors.initials: E
authors.lastname: Kihlström
mesh_terms: D001422:Bacterial Adhesion; D002168:Campylobacter fetus / Q000502:physiology*; D002852:Chromatography, Ion Exchange; D006801:Humans; D007422:Intestines / Q000382:microbiology; D012965:Sodium Chloride / Q000494:pharmacology; D012995:Solubility; D013499:Surface Properties; D014407:Tumor Cells, Cultured
chemical_list: D012965:Sodium Chloride