title: Acid hydrolysis of serum samples to increase detection of HIV antigen.
abstract: Sera from 32 HIV-infected patients and 20 controls were assayed for HIV antigen (HIV-Ag) and antibodies following acid hydrolysis. Acid hydrolysis, followed by neutralization immediately prior to the assay, was found to be a simple means to solubilize immune complexes and allowed recovery of 40-50% of complexed antigen. Following acid hydrolysis, HIV-Ag levels rose or became detectable in 22/32 patients and HIV IgG1-4 levels rose in 17/20 patients studied. The results show that complexed HIV-Ag may evade detection in HIV-infected patients.
authors.affiliation: Department of Virology, National Bacteriological Laboratory, Stockholm, Sweden.
authors.forename: T
authors.initials: T
authors.lastname: Mathiesen
authors.forename: V A
authors.initials: VA
authors.lastname: Sundqvist
authors.forename: J
authors.initials: J
authors.lastname: Albert
authors.forename: E
authors.initials: E
authors.lastname: Ohlsson
authors.forename: B
authors.initials: B
authors.lastname: Wahren
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology*; D000936:Antigen-Antibody Complex / Q000032:analysis; D015483:HIV Antibodies / Q000032:analysis; D015488:HIV Antigens / Q000032:analysis*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007074:Immunoglobulin G / Q000032:analysis / Q000145:classification
chemical_list: D000936:Antigen-Antibody Complex; D015483:HIV Antibodies; D015488:HIV Antigens; D007074:Immunoglobulin G