Role of the potential secondary structures in phage G4 origin of complementary DNA strand synthesis.
Phage G4 origin of complementary DNA strand synthesis (oric) consists of three stable stem-loop structures (I, II, and III). Mutant oric sequences with alterations in the structure of stem-loop II, stem-loop III, and the stem-loop II-III spacer region have been constructed and cloned into the filamentous phage vectors to assay their functional activity. Changes in the lowermost GC base pair in the stem of stem-loop III, in the 9-bp spacer region between the stems of stem-loops II and III, and in the loop of stem-loop II, impair or abolish in vivo oric function. The results suggest that recognition sequences for dnaG primase must be present in the loop of stem-loop II, and in the spacer region between the stems of stem-loops II and III.
Sakai
H
H
Department of Agricultural Chemistry, Kyoto University, Japan.
Hiasa
H
H
Iwamoto
K
K
Horimoto
T
T
Komano
T
T
Godson
G N
GN
D001435:Bacteriophages / Q000235:genetics*; D002021:Buffers; D003470:Culture Media; D004261:DNA Replication; D009154:Mutation; D009690:Nucleic Acid Conformation
D002021:Buffers; D003470:Culture Media