title: Imaging analysis and selected sequence monitoring of small peptides using planar chromatography/secondary ion mass spectrometry.
abstract: Mixtures of enkephalins and bradykinins contained on silica gel thin-layer chromatograms were imaged by secondary ion mass spectrometry. Spatial profiles of the sample distribution in the chromatographic plane were determined using a precision manipulator that moves the sample into and out of the primary beam generated in a liquid metal gallium source. For instance, spatial profiles are conveniently measured by monitoring the protonated molecule [M + H]+ of each compound or, in a selected-sequence monitoring experiment, by monitoring a sequence ion common to the members of each peptide group. The latter experiment is useful for quick location and identification of a class of peptides separated by planar chromatography, including thin-layer chromatography and electrophoresis.
authors.affiliation: Department of Chemistry, Indiana University, Bloomington 47405.
authors.forename: J C
authors.initials: JC
authors.lastname: Dunphy
authors.forename: K L
authors.initials: KL
authors.lastname: Busch
mesh_terms: D001920:Bradykinin / Q000032:analysis; D002855:Chromatography, Thin Layer; D004745:Enkephalins / Q000032:analysis; D013058:Mass Spectrometry / Q000379:methods; D010455:Peptides / Q000032:analysis*
chemical_list: D004745:Enkephalins; D010455:Peptides; D001920:Bradykinin