title: An embryonic-like myosin heavy chain is transiently expressed in nodal conduction tissue of the rat heart.
abstract: In the bovine nodal conduction tissue we have described the existence of a novel cardiac myosin isoform, immunologically related to the myosin types expressed during skeletal muscle development. Using different monoclonal antibodies specific for the embryonic and the neonatal skeletal myosin heavy chain types we investigated the myosin composition of the rat sino-atrial and atrio-ventricular nodes. We find that nodal conduction tissue fibers of the rat heart contain a distinct cardiac myosin isoform antigenically similar to the skeletal embryonic myosin heavy chain. The expression of this myosin isoform in nodal tissue appears to be developmentally regulated and partially controlled by thyroid hormone. Reactive cardiac fibers were detected in the nodal regions only during fetal development and a few days after birth, whereas very rare labelled fibers could be observed in the adult nodes. This myosin type does not represent a primordial cardiac myosin isoform since it was not detected in the embryonic heart before 13.5 days of gestation. When congenital hypothyroidism was induced in rats, the post-natal disappearance of reactive fibers in the nodal regions was delayed. On the other hand, hypothyroidism induced in the adult rats did not change the number of the reactive nodal fibers with respect to the euthyroid hearts.
authors.affiliation: Institute of General Pathology, University of Padova, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Gorza
authors.forename: L
authors.initials: L
authors.lastname: Saggin
authors.forename: S
authors.initials: S
authors.lastname: Sartore
authors.forename: S
authors.initials: S
authors.lastname: Ausoni
mesh_terms: D000818:Animals; D003409:Congenital Hypothyroidism; D006321:Heart / Q000196:embryology; D006329:Heart Conduction System / Q000032:analysis* / Q000196:embryology / Q000276:immunology; D007037:Hypothyroidism / Q000276:immunology*; D008297:Male; D009206:Myocardium / Q000032:analysis*; D009218:Myosins / Q000032:analysis* / Q000276:immunology; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D009218:Myosins