The cytotoxic effect of methotrexate as evaluated by colony-forming activity of hemopoietic and tumor cells.
The response of hemopoietic and/or lymphosarcoma cells to methotrexate has been investigated. The proliferative capacity of hemopoietic tissue was measured by exogenous CFU assay. It was inhibited by a six times lower amount of MTX than the proliferative activity of lymphosarcoma cells as evaluated by liver-colony assay. The natural resistance of LS/BL lymphosarcoma cells to MTX might be attributed to the DHFR gene amplification resulting from DNA rearrangements appearing during malignant transformation.
Jurásková
V
V
Institute of Biophysics, Czechoslovak Academy of Sciences, Brno.
D000818:Animals; D003114:Colony-Forming Units Assay; D004351:Drug Resistance; D006412:Hematopoietic Stem Cells / Q000187:drug effects*; D007928:Lethal Dose 50; D008228:Lymphoma, Non-Hodgkin / Q000188:drug therapy*; D008727:Methotrexate / Q000633:toxicity*; D051379:Mice; D008815:Mice, Inbred Strains
D008727:Methotrexate