Antiferritin antibodies in immunoscintigraphic detection of human tumor xenografts.
Affinity-purified antibodies against human placental ferritin and their F(ab)2 fragments labeled with 131I were examined for suitability for radioimmunodetection of ferritin-containing tumors. The nude mouse model (BALB/c, nu/nu) with xenografts of HeLa cell tumors and human adenocarcinoma of the rectum (with proven ferritin content) was used. Gamma-camera imaging and tissue distribution studies revealed that both kinds of tumor selectively accumulate antiferritin antibodies and their fragments. In large necrotic tumors nonspecific uptake of radiolabeled normal IgG occurred, but otherwise there was no tumor localisation. This study, in accordance with the literature, confirms the utility of antiferritin antibodies for the detection of human tumors in an animal model.
Koprivová
H
H
Institute of Biophysics and Nuclear Medicine, Faculty of General Medicine, Charles University Prague, CSSR.
Novák
F
F
Poucková
P
P
Dvorák
P
P
Seblová
J
J
Svihovcová
P
P
Dusková
J
J
Pospísil
J
J
Dienstbier
Z
Z
D000230:Adenocarcinoma / Q000000981:diagnostic imaging*; D000818:Animals; D000906:Antibodies / Q000276:immunology*; D005293:Ferritins / Q000276:immunology*; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000276:immunology*; D007457:Iodine Radioisotopes; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D011877:Radionuclide Imaging; D012004:Rectal Neoplasms / Q000175:diagnosis*
D000906:Antibodies; D007140:Immunoglobulin Fab Fragments; D007457:Iodine Radioisotopes; D005293:Ferritins