Optimization of fluorescence detection for the determination of labetalol in plasma by high-performance liquid chromatography.
A sensitive reversed-phase high-performance liquid chromatographic method for the determination of labetalol has been developed. A mobile phase consisting of citrate buffer (pH 6.5), acetonitrile and 2-propanol and an RP-8 column were used. The sensitivity of the fluorescence detection was enhanced to 1 ng/ml of labetalol in plasma by optimizing the emitted light. General guidelines for optimization of fluorescence detection are discussed.
Ostrovská
V
V
Drug Research Institute, Modra, Czechoslovakia.
Svobodová
X
X
Pechová
A
A
Kusala
S
S
Svoboda
M
M
D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007741:Labetalol / Q000097:blood*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
D007741:Labetalol