Thin-layer chromatographic enantiomeric resolution via ligand exchange.
A thin-layer chromatographic technique for the separation of proteinogenic and non-proteinogenic amino acids, dipeptides and alpha-hydroxy acids is described. Other examples are given from the field of alpha-methyl, N-alkyl and halogenated amino acids. The separation of the enantiomers is achieved, without derivatization, by means of ligand exchange on a reversed-phase silica gel as stationary phase, which is covered with a chiral selector (proline derivative). The resolution is so good that the respective enantiomers can be determined at trace levels (greater than or equal to 0.25%). The proposed method is simple, inexpensive and needs no sophisticated instruments.
Günther
K
K
Fachbereich Forschung Chemie, Degussa AG, Hanau, F.R.G.
D000596:Amino Acids / Q000302:isolation & purification; D002855:Chromatography, Thin Layer / Q000379:methods*; D002942:Circular Dichroism; D008024:Ligands; D012015:Reference Standards; D012997:Solvents; D013237:Stereoisomerism
D000596:Amino Acids; D008024:Ligands; D012997:Solvents