Effects of alpha-tocopherol-nicotinate administration on the microdynamics of phospholipids of erythrocyte membranes in human subjects.
The effects of alpha-tocopherol-nicotinate on the microdynamic parameters, membrane viscosity, and wobbling angle of phospholipid molecules of healthy human erythrocyte membranes were studied with a nanosecond time-resolved fluorometer. The incorporation of alpha-tocopherol was achieved by oral administration of alpha-tocopherol-nicotinate for one month with dosage of 400 mg/day. The increase in alpha-tocopherol concentration in erythrocytes was confirmed in two subjects. The membrane viscosity in seven healthy subjects decreased significantly from 1.015 +/- 0.137 to 0.888 +/- 0.133 mPa.s. But no changes in the wobbling angle of phospholipid molecules were observed.
Koyama
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Section of Physiology, Hokkaido University, Sapporo, Japan.
Araiso
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D000328:Adult; D004161:Diphenylhexatriene; D004910:Erythrocyte Membrane / Q000032:analysis / Q000187:drug effects*; D005260:Female; D005453:Fluorescence; D006801:Humans; D007202:Indicators and Reagents; D008297:Male; D008560:Membrane Fluidity / Q000187:drug effects; D008563:Membrane Lipids / Q000097:blood / Q000378:metabolism*; D008875:Middle Aged; D009539:Nicotinic Acids / Q000494:pharmacology*; D009996:Osmotic Fragility / Q000187:drug effects; D010743:Phospholipids / Q000097:blood / Q000378:metabolism*; D014810:Vitamin E / Q000031:analogs & derivatives* / Q000494:pharmacology
D007202:Indicators and Reagents; D008563:Membrane Lipids; D009539:Nicotinic Acids; D010743:Phospholipids; D014810:Vitamin E; D004161:Diphenylhexatriene; C012581:alpha-tocopheryl nicotinate