Simple method for the culture of glial cells from embryonic rat brain: implications for regional heterogeneity and the radial glial lineage.
A simple method is described for the production of glial cell cultures from specific regions of the day 13-15 embryonic rat brainstem and midbrain based on differential cell attachment to a relatively nonadhesive substrate, which inhibits the growth of neurons. Regional differences in the ability of specific populations of brainstem and midbrain cells to attach and spread on the substrate suggest that embryonic glial populations may differ in their cell surface properties even when they derive from the same general area of the developing brain. Based on observations of the spatiotemporal distribution of radial-like glial cells and astrocytes with time in vitro, we suggest that this culture system may prove useful for investigation of the radial glial lineage.
Wilkie
M B
MB
Department of Cell Biology and Anatomy, University of North Carolina School of Medicine, Chapel Hill 27599.
Lauder
J M
JM
D000818:Animals; D001933:Brain Stem / Q000166:cytology / Q000196:embryology* / Q000378:metabolism; D002448:Cell Adhesion; D002478:Cells, Cultured; D046508:Culture Techniques / Q000379:methods*; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D008636:Mesencephalon / Q000166:cytology / Q000196:embryology* / Q000378:metabolism; D009457:Neuroglia / Q000166:cytology* / Q000378:metabolism; D051381:Rats
D005904:Glial Fibrillary Acidic Protein