[Automated synthesis of oligodeoxyribonucleotides with terminal phosphate groups].
3'- and 5'-phosphorylated oligodeoxyribonucleotides have been synthesized on the "Victoria-4M" automatic synthesizer by phosphoramidite method. Two approaches have been suggested: introduction of a terminal ribo-unit as a potential source of phosphate group or the use of hydroxyl-containing polymer supports which loose the end product of the synthesis via beta-elimination reaction. Yields of oligonucleotides obtained according to both schemes proved to be almost identical. Using the first approach, oligonucleotides containing units with altered configuration of the sugar (xylo-thymidine and arabino-uridine) have been obtained.
Volkov
E M
EM
Romanova
E A
EA
Krug
A
A
Oretskaia
T S
TS
Potapov
V K
VK
D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis*; D010710:Phosphates
D009838:Oligodeoxyribonucleotides; D010710:Phosphates
Avtomticheskiĭ sintez oligodezoksiribonukleotidov s kontsevymi fosfatnymi gruppami.