title: DNA ploidy in thin melanoma.
abstract: DNA ploidy in benign nevi (BN), thin non-metastasizing melanomas (TNM) and thin metastasizing melanomas (TMM) was investigated using an image analyser to determine whether characteristics such as nuclear area (NA) and nuclear integrated optical density (IOD) could be used to distinguish between these lesions. NA measurements showed significant differences between samples of nevus cells and melanoma cells and nuclear IOD differences were significant between TNM and TMM samples. Differences in NA and nuclear IOD were detected across the three groups (BN, TNM and TMM) but the large variability within samples and within groups indicate further studies would be necessary to determine the usefulness of these results in terms of the rate of correct group classification of a single sample for diagnostic purposes.
authors.affiliation: Discipline of Pathology, Faculty of Medicine, University of Newcastle.
authors.forename: I M
authors.initials: IM
authors.lastname: Zardawi
authors.forename: L
authors.initials: L
authors.lastname: Jarvis
authors.forename: P
authors.initials: P
authors.lastname: Hersey
authors.forename: L
authors.initials: L
authors.lastname: Francis
authors.forename: H
authors.initials: H
authors.lastname: Shaw
authors.forename: J
authors.initials: J
authors.lastname: Grace
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002467:Cell Nucleus / Q000648:ultrastructure; D002648:Child; D004273:DNA, Neoplasm / Q000032:analysis*; D005260:Female; D006801:Humans; D008297:Male; D008545:Melanoma / Q000235:genetics / Q000473:pathology* / Q000556:secondary; D008875:Middle Aged; D009506:Nevus / Q000235:genetics / Q000473:pathology; D011003:Ploidies; D012189:Retrospective Studies; D012878:Skin Neoplasms / Q000235:genetics / Q000473:pathology*
chemical_list: D004273:DNA, Neoplasm