[Changes in the amino acid sequence of hemagglutinin during sequential adaptation of human influenza virus A to replication in mice].
The primary structure of hemagglutinin (HA) gene of Influenza virus A/USSR/90/77 (H1N1) variants after 3 and 11 passages has been determined. In the HA1 coding region of mice-adapted virus (11 passages) there are two amino acid substitutions: Thr 89----Ala and Asn 127----Asp. At the first stage of adaptation (3-rd passage) only a single mutation was detected: Asn 127----Asp. The adaptation is accompanied by the loss of specific carbohydrate attachment sites adjacent to the receptor-binding site located at HA1 subunit with a concomitant variation in antigenicity.
Grinev
A A
AA
Samokhvalov
E I
EI
Petrov
N A
NA
Vasilenko
S K
SK
Iuferov
V P
VP
D000220:Adaptation, Biological; D000595:Amino Acid Sequence; D000818:Animals; D006389:Hemagglutinins, Viral / Q000032:analysis / Q000235:genetics*; D006801:Humans; D009980:Influenza A virus / Q000235:genetics* / Q000502:physiology; D051379:Mice; D008969:Molecular Sequence Data; D014779:Virus Replication
D006389:Hemagglutinins, Viral
Izmeneniia aminokislotnoĭ posledovatel'nosti gemaggliutinina pri posledovatel'nykh étapakh adaptatsii virusa grippa A cheloveka k razmnozheniiu v organizme mysheĭ.