The molecular weight of the extracellular haemoglobin of Lumbricus terrestris determined by electron microscopy.
1. The mol. wt of the extracellular haemoglobin of the oligochaete Lumbricus terrestris was determined by counting in negatively stained electron micrographs. 2. The value obtained using apoferritin as a mol. wt standard is (3.8 +/- 0.3) x 10(6), in agreement with recent determinations employing different physical methods. 3. We conclude that all annelid extracellular haemoglobins and chlorocruorins which have the same dimensions as Lumbricus haemoglobin probably have the same mol. wt.
Vinogradov
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Biochemistry Department, School of Medicine, Wayne State University, Detroit, MI 48201.
Kolodziej
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D000818:Animals; D001052:Apoferritins / Q000592:standards / Q000648:ultrastructure; D005110:Extracellular Space / Q000032:analysis; D006454:Hemoglobins / Q000648:ultrastructure*; D008854:Microscopy, Electron; D008970:Molecular Weight; D009835:Oligochaeta / Q000032:analysis*
D006454:Hemoglobins; D001052:Apoferritins