title: Distribution of postsynthetic methylation sites in Physarum histone H1.
abstract: Using limited chymotrypsin and trypsin digestion of isolated Physarum histone H1 labeled in vivo in postsynthetically added N epsilon-methyl groups of lysine we show that: --there is no postsynthetic methylation in the central globular domain of H1, --a moderate number of methylated sites occurs in the N-terminal fragment and the part of the C-terminal fragment directly adjacent to the globular domain (the main site of interphase phosphorylation), --the most intensively methylated region occurs within the sequence located in an extended part of the C-terminal fragment, distant to the globular domain and the main site of interphase phosphorylation.
authors.affiliation: Department of Biochemistry, Warsaw University, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Jerzmanowski
authors.forename: J
authors.initials: J
authors.lastname: Moraczewska
mesh_terms: D001665:Binding Sites; D002843:Chromatin / Q000378:metabolism; D005656:Fungal Proteins / Q000378:metabolism*; D006657:Histones / Q000378:metabolism*; D008745:Methylation; D010446:Peptide Fragments / Q000378:metabolism; D010804:Physarum / Q000378:metabolism*
chemical_list: D002843:Chromatin; D005656:Fungal Proteins; D006657:Histones; D010446:Peptide Fragments