title: Effect of a combination of copper and hexetidine on the acidogenicity and copper accumulation in dental plaque in vivo.
abstract: A double-blind crossover study on 4 adult volunteers was performed to evaluate the effect of hexetidine on Cu2+ accumulation in dental plaque as well as a possible enhanced effect of copper on inhibition of acid production in the presence of hexetidine. The experimental period was 5 days. No oral hygiene was allowed, and sucrose-containing chewing gum was used to enhance plaque formation during the test period. In order to evaluate the effect on pH, the test persons rinsed with a 15% glucose (w/v) solution on the 5th experimental day. Plaque pH values recorded before and 5 min after the rinse served as control values. One hour later the test persons rinsed with 10 ml of the test solutions for 1 min. Glucose rinses with pH measurements 5 min after the rinse were carried out 0, 3, and 7 h after the test agents were used. The combination of 1.0 mM copper and 2.0 mM hexetidine gave a significant (p less than 0.05) inhibition of acid production at all times compared both to the controls and to each of the test agents separately. Plaque samples were collected with a toothpick immediately before a 1-min rinse with 10 ml of the test solutions. Subsequent plaque samples were taken 5 min and 3 and 8 h after a rinse from corresponding tooth surfaces. Dry weight was estimated, the plaque bacteria digested by HNO3, and the amount of Cu2+ determined by atomic absorption.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Microbiology, Dental Faculty, University of Oslo, Norway.
authors.forename: J
authors.initials: J
authors.lastname: Grytten
authors.forename: A
authors.initials: A
authors.lastname: Aamdal Scheie
authors.forename: J
authors.initials: J
authors.lastname: Afseth
mesh_terms: D000143:Acids / Q000378:metabolism; D000328:Adult; D003300:Copper / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology*; D003773:Dental Plaque / Q000378:metabolism*; D004311:Double-Blind Method; D004338:Drug Combinations; D006590:Hexetidine / Q000008:administration & dosage / Q000494:pharmacology*; D006801:Humans; D006863:Hydrogen-Ion Concentration
chemical_list: D000143:Acids; D004338:Drug Combinations; D003300:Copper; D006590:Hexetidine