title: Light microscopic morphometric analysis of castration effects in the different lobes of the rat prostate.
abstract: The morphologic effects of androgen deprivation in the different lobes of the rat prostate were examined by light microscopic morphometry. The prostates of Wistar male rats (260-340 g) were fixed in situ by glutaraldehyde perfusion in castrated animals 1 week after gonadectomy and in intact animals. The ventral (VP), dorsal (DP), and lateral (LP) lobes as well as the coagulating gland (CG) were dissected out, weighed, and processed for light microscopy. Using stereologic methods the following parameters were estimated for each lobe: volume fraction of connective tissue, epithelium and glandular lumina, average epithelial height, average epithelial cell volume, and total number of epithelial cells. Castration leads to a 58-76% reduction of the wet weight of all prostatic lobes. The decrease of glandular tissue is greater in VP than in LP, DP, and CG. In VP and LP, there is a 39-45% reduction of the epithelial height, and this effect is less pronounced in DP and CG. For all lobes, the shrinkage of average epithelial cell volume is in the same range (25-30%). Moreover, in VP and LP, there is a 70% reduction of the total number of cells, whereas the reduction is less in DP and CG. It thus seems that the reduction of prostatic epithelial tissue mass upon castration is due to a reduction of the number of cells as well as a reduction of the volume of individual cells. VP and LP appear to be more androgen-dependent than DP and CG.
authors.affiliation: Institute of Cancer Research, University Hospital, Trondheim, Norway.
authors.forename: K M
authors.initials: KM
authors.lastname: Kiplesund
authors.forename: J
authors.initials: J
authors.lastname: Halgunset
authors.forename: H E
authors.initials: HE
authors.lastname: Fjösne
authors.forename: A
authors.initials: A
authors.lastname: Sunde
mesh_terms: D000818:Animals; D008297:Male; D008433:Mathematics; D009919:Orchiectomy / Q000009:adverse effects*; D009929:Organ Size; D010477:Perfusion; D011467:Prostate / Q000473:pathology*; D051381:Rats; D011919:Rats, Inbred Strains