A domain that assumes a Z-conformation includes a specific deletion in some cloned variants of a complex satellite.
Sequence analyses show that deletions of 10 and 12 bp occur at homologous sites in a domain that is rich in alternating purines and pyrimidines (Pu/Py) in B42 and EXT, two cloned variants of a complex satellite DNA. A 3-bp deletion occurs 27 bp upstream from the site of the specific deletions in B42 and RU, a third cloned satellite variant that has not suffered the 10-bp deletion. Under torsional stress, the Pu/Py-rich domain adopts a Z-conformation as shown by (i) inhibition of cutting at a BssHII site that accounts for 2/5 of a 15-bp tract of pure Pu/Py in the domain; (ii) binding of polyclonal and monoclonal anti-Z-DNA antibodies to the domain; and (iii) antibody stabilization and subsequent relaxation of the Z-region.
Fowler
R F
RF
University of Tennessee-Oak Ridge Graduate School of Biomedical Sciences, Biology Division, Oak Ridge National Laboratory 37831.
Stringfellow
L A
LA
Skinner
D M
DM
D000818:Animals; D000911:Antibodies, Monoclonal; D001483:Base Sequence; D003386:Brachyura / Q000235:genetics; D002872:Chromosome Deletion; D003001:Cloning, Molecular; D004274:DNA, Recombinant; D004276:DNA, Satellite / Q000235:genetics* / Q000276:immunology; D014644:Genetic Variation; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010957:Plasmids
D000911:Antibodies, Monoclonal; D004274:DNA, Recombinant; D004276:DNA, Satellite