Protective effects of vitamins E and C on erythrocytes in blood preserved in ACD solution and stored at 4 degrees C.
Vitamins E and C were used for the enrichment of the fluid for blood preservation. Whole blood with added ACD solution and vitamins E and C was stored at 4 degrees C for 21 days. The performed determinations included: haematocrit, osmotic fragility of the erythrocytes, ATP and 2,3-DPG content in the erythrocytes, and echinocyte count. A protective effect of these vitamins on erythrocytes was manifested by the decreased osmotic fragility, the steep fall of 2,3-DPG compared with the blood preserved in the vitamin-free ACD solution.
Kopeć-Szlezak
J
J
Department of Physiopathology, Institute of Haematology, Warsaw, Poland.
Grabarczyk
M
M
Szczepańska
I
I
Furman
S
S
D019794:2,3-Diphosphoglycerate; D000255:Adenosine Triphosphate / Q000097:blood; D001205:Ascorbic Acid / Q000494:pharmacology*; D001772:Blood Cell Count; D001793:Blood Preservation / Q000379:methods*; D004163:Diphosphoglyceric Acids / Q000097:blood; D004912:Erythrocytes / Q000187:drug effects*; D005615:Freezing; D006400:Hematocrit; D006801:Humans; D009996:Osmotic Fragility / Q000187:drug effects; D014810:Vitamin E / Q000494:pharmacology*
D004163:Diphosphoglyceric Acids; D019794:2,3-Diphosphoglycerate; D014810:Vitamin E; D000255:Adenosine Triphosphate; D001205:Ascorbic Acid