title: Method of genomic DNA cloning by the combination of cosmid shuttle vector and monoclonal antibody.
abstract: We report here the strategy to isolate the DNA fragment of any species origin which encodes cell surface antigen by using cosmid library transfection and cell sortings with a monoclonal antibody. We took the mouse melanoma antigen defined by monoclonal antibody as a model system and rescued the genomic DNA by in vitro packaging, showing the feasibility of this procedure.
authors.affiliation: Department of Immunology, School of Medicine, Chiba University.
authors.forename: M
authors.initials: M
authors.lastname: Tagawa
authors.forename: T
authors.initials: T
authors.lastname: Ito
authors.forename: M
authors.initials: M
authors.lastname: Kanno
authors.forename: A
authors.initials: A
authors.lastname: Okitsu
authors.forename: T
authors.initials: T
authors.lastname: Sakamoto
authors.forename: K
authors.initials: K
authors.lastname: Imai
authors.forename: I
authors.initials: I
authors.lastname: Kuwabara
authors.forename: H
authors.initials: H
authors.lastname: Koseki
authors.forename: H
authors.initials: H
authors.lastname: Matsubara
authors.forename: Y F
authors.initials: YF
authors.lastname: Lau
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000235:genetics*; D000954:Antigens, Surface / Q000235:genetics; D002478:Cells, Cultured; D003001:Cloning, Molecular / Q000379:methods*; D003360:Cosmids; D004247:DNA / Q000235:genetics*; D006801:Humans; D008546:Melanoma, Experimental / Q000235:genetics; D051379:Mice; D014162:Transfection
chemical_list: D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D004247:DNA