title: Picosecond transient absorption study of photodissociated carboxy hemoglobin and myoglobin.
abstract: The optical transient absorption spectra at 30 ps and 6.5 ns after photolysis are compared for both carboxy hemoglobin (HbCO) and carboxy myoglobin (MbCO). Both 355- and 532-nm excitation pulses were used. In all cases the shapes of the optical difference spectra thus generated are stationary over the complete time-scale studied. The photolysis spectra for MbCO are not significantly different from the equilibrium difference spectra generated on the same picosecond spectrometer when measured to an accuracy of +/- 0.5 nm. In addition, spectral parameters for delegated HbCO generated on the same spectrometer but detected by two different techniques, either by a Vidicon detector or point by point with photomultiplier tubes, are reported; the results are different from some of the previously reported picosecond experiments.
authors.affiliation: Department of Chemistry, University of Pennsylvania, Philadelphia 19104-6323.
authors.forename: S M
authors.initials: SM
authors.lastname: Janes
authors.forename: G A
authors.initials: GA
authors.lastname: Dalickas
authors.forename: W A
authors.initials: WA
authors.lastname: Eaton
authors.forename: R M
authors.initials: RM
authors.lastname: Hochstrasser
mesh_terms: D000818:Animals; D002263:Carboxyhemoglobin / Q000378:metabolism* / Q000528:radiation effects; D006801:Humans; D007700:Kinetics; D009211:Myoglobin / Q000378:metabolism* / Q000528:radiation effects; D010782:Photolysis; D013056:Spectrophotometry, Ultraviolet; D014907:Whales
chemical_list: D009211:Myoglobin; C018013:carboxymyoglobin; D002263:Carboxyhemoglobin