[Effect of combined transfer of irradiated allo-lymphocytes and LAK cells on pulmonary metastasis of mammary carcinoma (SST-2) in SHR rats].
We have attempted to prevent and treat experimental pulmonary metastasis of a rat mammary carcinoma (SST-2) by adopting transfer of LAK cells and irradiated allogeneic spleen cells (as an allogeneic immune stimulator). Transfer experiments were carried out by the following schedule: SST-2 cells were inoculated intravenously on day 0, irradiated allogeneic spleen cells were transferred intravenously on day -5, 1 and 8, and LAK cells were transferred on day 2, 5 and 7. In the control groups: the average number of metastatic nodules in the lungs were 180.5, 62.0, 10.2 in the groups of allogeneic lymphocytes alone and LAK cells alone in experiment 1, and 81.2, 21.0, 3.8 in experiment 2, respectively. In the combined transfer group, the average metastatic nodules were 1.3 in experiment 1 and 0 in experiment 2, and 17 out of the total 20 rats were completely free from metastasis. Interleukine 2 (IL-2) level in the serum increased gradually and reached a peak on day 3 and thereafter, declined after the 2nd transfer of allogeneic lymphocytes. We postulate that allogeneic lymphocytes transferred are able to stimulate a release of endogenous IL-2 which supports and augments antitumor activity of LAK cells.
Li
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SZ
Cancer Institute, Haerbin Medical College, Haerbin.
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D000818:Animals; D005260:Female; D007116:Immunization, Passive; D007694:Killer Cells, Natural / Q000528:radiation effects / Q000637:transplantation*; D008175:Lung Neoplasms / Q000556:secondary / Q000628:therapy*; D008297:Male; D008325:Mammary Neoplasms, Experimental; D051379:Mice; D008810:Mice, Inbred C57BL; D009368:Neoplasm Transplantation; D051381:Rats; D011918:Rats, Inbred SHR; D013154:Spleen / Q000166:cytology