title: Do motilin and pancreatic polypeptide regulate duodenal bile acid delivery?
abstract: The plasma levels of the enteric hormones, motilin and pancreatic polypeptide, cycle in association with fasting intestinal motility and are altered by feeding. Intravenous administration of motilin causes gallbladder contraction and increased sphincter of Oddi phasic motor activity, whereas pancreatic polypeptide causes gallbladder relaxation. To determine if endogenous plasma levels of motilin and pancreatic polypeptide control sphincter of Oddi and gallbladder motility, and regulate duodenal bile acid delivery, we measured during fasting and after feeding the correlation between (a) changes in plasma motilin or pancreatic polypeptide, and (b) the duodenal delivery of a steady-state hepatic output of radiolabelled bile acid. Four dogs were prepared with duodenal cannulas. Duodenal motility was recorded manometrically. Plasma levels of pancreatic polypeptide and motilin were determined during a full cycle of the migrating myoelectric complex for 20 min before and 40 min after ingestion of a standard meal. To assess the effect of the sphincter of Oddi and the gallbladder together, or the gallbladder alone on duodenal bile acid delivery, the dogs received a continuous i.v. infusion of [14C]taurocholic acid (TCA); duodenal delivery of TCA was quantitated with the sphincter of Oddi intact using duodenal marker perfusion, or with the sphincter of Oddi cannulated and zero outflow resistance. In the interdigestive period with the sphincter of Oddi intact, only 0.1 (r2) of the variance of duodenal bile acid delivery can be predicted from the variance of motilin, and the correlation of plasma pancreatic polypeptide with duodenal TCA delivery is opposite that expected if pancreatic polypeptide caused gallbladder relaxation.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Pediatrics, Faculty of Medicine, University of Calgary, Alta., Canada.
authors.forename: R B
authors.initials: RB
authors.lastname: Scott
authors.forename: S C
authors.initials: SC
authors.lastname: Diamant
authors.forename: G R
authors.initials: GR
authors.lastname: Greenburg
mesh_terms: D000818:Animals; D001647:Bile Acids and Salts / Q000502:physiology*; D004285:Dogs; D004386:Duodenum / Q000502:physiology*; D009037:Motilin / Q000097:blood / Q000502:physiology*; D010191:Pancreatic Polypeptide / Q000097:blood / Q000502:physiology*; D013656:Taurocholic Acid / Q000502:physiology
chemical_list: D001647:Bile Acids and Salts; D009037:Motilin; D010191:Pancreatic Polypeptide; D013656:Taurocholic Acid