ELISA for the measurement of sheep antibodies to the capsular antigens of Pasteurella haemolytica serotypes.
An enzyme-linked immunosorbent assay utilising direct binding of capsular polysaccharide antigens to polystyrene immunoassay plates was used to measure sheep antibodies to Pasteurella haemolytica A1, A2 and A6 serotypes. Low level cross reactivity occurred between A2 antigen and heterologous antisera. A strong unilateral cross reaction between A1 antigen and anti-A6 serum was abolished by absorption. These reactions suggest shared capsular antigens between serotypes.
Fodor
L
L
Moredun Research Institute, Edinburgh.
Donachie
W
W
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis*; D000942:Antigens, Bacterial / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D010325:Pasteurella / Q000276:immunology*; D012756:Sheep / Q000276:immunology*
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial