Platelet antibody detection using frozen pooled human lymphocyte antigen-typed platelets as target cells.
The immunoreactivity of five sera containing either iso-, auto- or allo-antiplatelet antibodies to frozen-thawed human lymphocyte antigen (HLA)-typed pooled platelets was assessed to determine the practicality of using such a platelet preparation in an immunofluorescent platelet antibody system. The platelet reagent was analyzed over an 8-week period after initial freezing and storage. Mixtures of platelets with each serum or dilutions of it were initially incubated for 1 hour, then extensively washed and incubated with fluorescein-conjugated goat F(ab')2 antihuman IgG, A, M. After repeat washing, each mixture was then analyzed using a flow cytometer to determine the relative amount of fluorescent antibody bound. Not only was each of the five sera consistently positive for the presence of an anti-platelet antibody, but the extent of immunoreactivity for each remained relatively stable over the 8-week period of analysis. The results suggest that frozen pooled HLA-typed platelets should be an invaluable reagent for screening serum samples for the presence of antiplatelet antibodies in patients who are thrombocytopenic or who are refractory to platelet transfusion therapy.
Lazarchick
J
J
Department of Pathology, Medical University of South Carolina, Charleston 29425.
Jones
T J
TJ
Russell
R J
RJ
Hall
S A
SA
D001792:Blood Platelets / Q000276:immunology*; D001793:Blood Preservation; D005260:Female; D005434:Flow Cytometry; D005615:Freezing; D006680:HLA Antigens / Q000032:analysis*; D006801:Humans; D008297:Male
D006680:HLA Antigens