Hybridization of DNA probes to A. marginale isolates from different sources and detection in Dermacentor andersoni ticks.
DNA from the Washington, South-Idaho, Virginia and Florida isolates of Anaplasma marginale was hybridized to probes specific for Anaplasma centrale and A. marginale. The A. centrale probes AC-2 and AC-4 hybridized to identical bands on all of these isolates. The hybridization patterns suggests that the Virginia, Florida and the South African isolates are similar. A number of bands were obtained with the Washington isolate which differed from those obtained with the other isolates. Probe AC-2 could be developed to identify relatedness among Anaplasma isolates. Probe AC-2 detected A. marginale DNA in midgut material from infected Dermacentor andersoni ticks. No hybridization was obtained with DNA from salivary gland tissues from these infected ticks.
Ambrosio
R E
RE
Molecular Biology Section, Veterinary Research Institute, Onderstepoort.
Visser
E S
ES
Koekhoven
Y
Y
Kocan
K M
KM
D000709:Anaplasma / Q000235:genetics / Q000302:isolation & purification*; D000818:Animals; D015342:DNA Probes; D004269:DNA, Bacterial; D003870:Dermacentor / Q000382:microbiology*; D009693:Nucleic Acid Hybridization; D013987:Ticks / Q000382:microbiology*
D015342:DNA Probes; D004269:DNA, Bacterial