title: Effect of drugs in vitro on lysosomal enzyme activities in bovine retinal pigment epithelial cells.
abstract: We examined in vitro the effect of several drugs on the activities of lysosomal enzymes in the crude extract of bovine retinal pigment epithelial cells. Acid phosphatase, beta-D-glucuronidase, N-acetyl-beta-D-glucosaminidase, alpha-L-fucosidase, and alpha-D-mannosidase were used as lysosomal enzymes. Sodium iodate at 10(-6), 10(-5) and 10(-4) M, and potassium iodate at 10(-5) and 10(-4) M inhibited acid phosphatase activity. Ferrous chloride at 10(-5) and 10(-4) M suppressed beta-D-glucuronidase activity. Ferric chloride, indomethacin, chloroquine, chlorpromazine, 5-fluorouracil, tobramycin, daunomycin, oxalate and epinephrine had no or only minimal inhibitory effects on the lysosomal enzyme activities examined.
authors.affiliation: Department of Ophthalmology, Shimane Medical University, Izumo, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Hayasaka
authors.forename: S
authors.initials: S
authors.lastname: Noda
authors.forename: T
authors.initials: T
authors.lastname: Setogawa
mesh_terms: D000818:Animals; D002417:Cattle; D005296:Ferrous Compounds / Q000494:pharmacology; D066298:In Vitro Techniques; D007452:Iodates / Q000494:pharmacology; D008247:Lysosomes / Q000201:enzymology*; D010857:Pigment Epithelium of Eye / Q000187:drug effects* / Q000201:enzymology; D011188:Potassium / Q000494:pharmacology; D017680:Potassium Compounds
chemical_list: D005296:Ferrous Compounds; D007452:Iodates; D017680:Potassium Compounds; C039693:potassium iodate; D011188:Potassium; C029451:ferrous chloride; C032285:sodium iodate