Single-stranded DNA fragments holding unusual conformation.
In the chemical synthesis of DNA, we found that the single-stranded DNA (ssDNA) fragments containing the sequence GCGAAAGC showed higher mobilities than the fragments without this sequence on a denaturing polyacrylamide gel electrophoresis. Physical structure of these DNA fragments was studied by enzyme digestion and optical analysis. The abnormal mobilities on electrophoresis seem to depend on an unusual conformation.
Hirao
I
I
Department of Industrial Chemistry, Faculty of Engineering, Tokyo, Japan.
Naraoka
T
T
Minami
H
H
Watanabe
K
K
Nishimura
Y
Y
Miura
K
K
D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D004277:DNA, Single-Stranded; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000302:isolation & purification
D004277:DNA, Single-Stranded; D009838:Oligodeoxyribonucleotides