Effects of charcoal-extracted serum as a growth medium supplement on in vitro development of mouse embryos.
Two-cell mouse embryos (N = 952) were cultured in modified Ham's F-10 medium supplemented with 15% charcoal-extracted serum or 15% nonextracted serum from 17 patients. Each sample was assayed independently and all experiments run in duplicate. Ten nonextracted samples inhibited development to the blastocyst stage compared to controls in F-10 alone. Charcoal extraction significantly improved (P less than 0.05) development compared to nonextracted serum in eight of these samples. Seven samples supported development and no difference was noted between F-10 alone and extracted and nonextracted serum-supplemented media in this group. In conclusion, charcoal extraction significantly reversed the "embryotoxic" effects of some sera in the two-cell mouse embryo model.
Padilla
S L
SL
Department of Obstetrics and Gynecology, Medical College of Georgia, Augusta.
Howe
A M
AM
Boldt
J P
JP
D000818:Animals; D001769:Blood; D002606:Charcoal; D002970:Cleavage Stage, Ovum; D003470:Culture Media; D005314:Embryonic and Fetal Development; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D008297:Male; D051379:Mice; D011247:Pregnancy
D003470:Culture Media; D002606:Charcoal