Determination of leukocyte elastase-inhibitor complexes and leukocyte neutral proteinase inhibitor by enzyme immunoassays. Leukocyte elastase-inhibitor complexes in porcine blood, III.
Sensitive enzyme immunoassays for the determination of total leukocyte neutral proteinase inhibitor and polymorphonuclear elastase-leukocyte neutral proteinase inhibitor complexes are described. The usable ranges of the standard curves were from 80 ng/l to 10 micrograms/l. The relative intra-assay coefficients of variation of the tests were between 2 and 4%, and the inter-assay coefficients of variation between 4 and 10%. In vitro and in vivo studies were performed with septic pigs and isolated leukocytes. The results show that leukocyte neutral proteinase inhibitor can be used in pigs as a parameter for the development of a septicaemia.
Geiger
R
R
Abteilung für Klinische Chemie und Klinische Biochemie in der Chirurgischen Klinik Innenstadt Universität, München.
Sokal
S
S
Siebeck
M
M
Hoffmann
H
H
Trefz
G
G
D000818:Animals; D001798:Blood Proteins; D004797:Enzyme-Linked Immunosorbent Assay; D011480:Protease Inhibitors / Q000097:blood*; D018805:Sepsis / Q000378:metabolism; D015843:Serpins; D013552:Swine
D001798:Blood Proteins; D011480:Protease Inhibitors; D015843:Serpins