Serodiagnosis of Streptococcus pneumoniae infection in guinea pigs by an enzyme-linked immunosorbent assay.
Guineapig antibodies to Streptococcus pneumoniae (SPN) serotype 19F were detected by an enzyme-linked immunosorbent assay using a simple procedure. In experimentally infected hosts, antibody was detectable as early as 2 to 3 weeks after infection, and high titres were maintained for a long period. Antibodies higher than 1:64 were regarded as specific. In a field study, high antibody titres were shown in SPN enzootic colonies in contrast to negative or low antibody titres in a majority of the animals from non-enzootic and SPF colonies.
Matsubara
J
J
Department of Veterinary Science, National Institute of Health, Tokyo, Japan.
Kamiyama
T
T
Miyoshi
T
T
Ueda
H
H
Saito
M
M
Nakagawa
M
M
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary*; D006168:Guinea Pigs / Q000276:immunology*; D011008:Pneumococcal Infections / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D012376:Rodent Diseases / Q000175:diagnosis*; D012698:Serologic Tests / Q000662:veterinary*
D000907:Antibodies, Bacterial