[Fractionation of chicken egg glycoproteins and their purification using preparative HPLC].
A new method of fractionation of hen egg glycoproteins has been developed. The procedure involves high-speed mass ion-exchange chromatography on ZetaPrep cartridges, differential precipitation, and ultrafiltration on "Minitan" tangential-flow system. Six fractions were obtained from egg white (ovomucin, avidin, riboflavin-binding glycoprotein RF-GPw, ovoinhibitor, ovalbumin-ovotransferrin, and ovomucoid fractions), and two fractions from egg yolk (riboflavin-binding glycoprotein RF-GPy and phosvitin fractions). Using ion-exchange HPLC on columns (150 X 21.5 mm) Protein PAK DEAE-5PW and SP-5PW, six homogenous glycoproteins (avidin, RF-GPw, ovalbumin, ovotransferrin, ovomucoid, and RF-GPy) were isolated in preparative quantities (0.1-1 g). Ion-exchange HPLC also resolves some glycoproteins' isoforms with different pI values.
Piskarev
V E
VE
Galenko
E L
EL
Likhosherstov
L M
LM
Derevitskaia
V A
VA
Kochetkov
N K
NK
D000818:Animals; D002645:Chickens; D002851:Chromatography, High Pressure Liquid; D004527:Egg Proteins / Q000302:isolation & purification*; D006023:Glycoproteins / Q000302:isolation & purification*; D014461:Ultracentrifugation
D004527:Egg Proteins; D006023:Glycoproteins
Fraktsionirovanie glikoproteinov kurinogo iaĭtsa i ikh ochistka s pomoshch'iu preparativnoĭ VEZhKh.