title: [Specificity of fragmentation of DNA from pBR322 plasmid by Ca,Mg-dependent endonuclease from cell nuclei of human lymphocytes].
abstract: Fragmentation of the plasmid pBR322 DNA by a purified preparation of Ca/Mg-dependent endonuclease has been studied. It was shown that on the first steps of reaction the double-stranded cuts are introduced into the superhelical DNA independent of singlestranded ones. The doublestranded cuts are introduced into superhelical and linear DNA in 12 sites enriched with GC-pairs, 9 of them include pentanucleotide CGCGG(CCGCC) that is functionally significant. Relaxation of the plasmid DNA by topoisomerase I blocks the sitespecific action of the enzyme. Ca/Mg-dependent endonuclease is concluded to be topologically dependent enzyme, possibly, participating in the recombination processes.
authors.forename: N N
authors.initials: NN
authors.lastname: Khodarev
authors.forename: A Iu
authors.initials: AIu
authors.lastname: Morozov
authors.forename: I A
authors.initials: IA
authors.lastname: Sokolova
authors.forename: S S
authors.initials: SS
authors.lastname: Aleksandrova
authors.forename: I I
authors.initials: II
authors.lastname: Votrin
mesh_terms: D001483:Base Sequence; D002467:Cell Nucleus / Q000201:enzymology; D004247:DNA / Q000235:genetics / Q000378:metabolism*; D004260:DNA Repair; D004706:Endodeoxyribonucleases / Q000378:metabolism*; D006801:Humans; D006868:Hydrolysis; D007700:Kinetics; D008214:Lymphocytes / Q000201:enzymology*; D008969:Molecular Sequence Data; D010957:Plasmids
chemical_list: D004247:DNA; D004706:Endodeoxyribonucleases; C029367:calcium magnesium dependent endodeoxyribonuclease
vernacular_title: Spetsifichnost' fragmentatsii DNK plazmidy pBR322 Ca/Mg-zavisimoĭ éndonukleazoĭ kletochnykh iader limfotsitov cheloveka.