title: Degranulation of peripheral blood neutrophils during haemoperfusion in chronic uraemics.
abstract: In 10 chronic uraemics activities of some lysosomal enzymes were determined in peripheral blood neutrophils and plasma during haemoperfusion with charcoal cartridge Adsorba 300 C (Gambro). Blood plasma activities of arylsulfatases, beta-N-acetylglucosaminidase and beta-glucuronidase did not significantly change during haemoperfusion, while lysozyme activity was significantly increasing. Neutrophil contents of these enzymes decreased. The determinations of these enzyme activities revealed a positive outlet-inlet difference in blood plasma and a negative outlet-inlet difference in neutrophils during the first 20 minutes of the procedure. The results suggest that during haemoperfusion degranulation of the peripheral blood neutrophils occurs.
authors.affiliation: 2nd Department of Internal Diseases, Institute of Medicine, WAM, Lódz, Poland.
authors.forename: K
authors.initials: K
authors.lastname: Trznadel
authors.forename: M
authors.initials: M
authors.lastname: Luciak
authors.forename: Z
authors.initials: Z
authors.lastname: Zbróg
authors.forename: Z
authors.initials: Z
authors.lastname: Kidawa
mesh_terms: D000118:Acetylglucosaminidase / Q000097:blood; D000328:Adult; D001192:Arylsulfatases / Q000097:blood; D002606:Charcoal; D002908:Chronic Disease; D005966:Glucuronidase / Q000097:blood; D006464:Hemoperfusion; D006801:Humans; D009113:Muramidase / Q000097:blood; D009504:Neutrophils / Q000201:enzymology*; D014511:Uremia / Q000201:enzymology* / Q000628:therapy
chemical_list: D002606:Charcoal; D001192:Arylsulfatases; D009113:Muramidase; D005966:Glucuronidase; D000118:Acetylglucosaminidase