alpha-Oligodeoxynucleotide stability in serum, subcellular extracts and culture media.
Degradation of a synthetic alpha-oligodeoxynucleotide was studied in order to compare its survival with naturally occurring beta-oligodeoxynucleotides in five systems used for antisense hybridization arrest experiments. In contrast to beta-oligodeoxynucleotides, alpha-oligodeoxynucleotides were not detectably degraded over 24 h at 37 degrees C in HeLa cell postmitochondrial cytoplasmic extract or RPMI 1640 with 10% fetal bovine serum, and showed significant survival after 24 h at 37 degrees C in rabbit reticulocyte lysate, fetal bovine serum and human serum.
Bacon
T A
TA
Department of Chemistry, University of South Florida, Tampa 33620.
Morvan
F
F
Rayner
B
B
Imbach
J L
JL
Wickstrom
E
E
D001483:Base Sequence; D003470:Culture Media; D004355:Drug Stability; D006367:HeLa Cells / Q000378:metabolism; D006801:Humans; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000097:blood / Q000378:metabolism*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics*; D013347:Subcellular Fractions / Q000378:metabolism
D003470:Culture Media; D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger