Growth dependent enzymatic profiles of some gram-negative nonfermentative bacteria of clinical significance.
A total of 734 strains of gram-negative nonfermentative bacteria (46 species and biochemically defined groups) of the genera Pseudomonas, Alcaligenes, Bordetella, Moraxella, Acinetobacter, Agrobacterium, and Flavobacterium were investigated for their ability to hydrolyze 25 different chromogenic substrates. All tests were carried out in growth-stimulating media. Results were read photometrically and evaluated automatically following a 24-h incubation. Many of the 46 different species and biochemical groups exhibited uniform patterns of enzyme production. Some of the enzyme tests may serve as additional valuable tools for differential diagnosis of the organisms investigated. In combination with other biochemical tests, qualitative enzyme demonstration tests can facilitate the identification of gram-negative nonfermentative bacteria.
Kämper
P
P
Fachgebiet Hygiene, Technische Universität Berlin.
Dott
W
W
D000150:Acinetobacter / Q000201:enzymology; D000421:Alcaligenes / Q000201:enzymology / Q000302:isolation & purification; D001884:Bordetella / Q000201:enzymology / Q000302:isolation & purification; D002863:Chromogenic Compounds / Q000378:metabolism; D003470:Culture Media; D005417:Flavobacterium / Q000201:enzymology; D006090:Gram-Negative Bacteria / Q000201:enzymology* / Q000254:growth & development / Q000302:isolation & purification; D006868:Hydrolysis; D009016:Moraxella / Q000201:enzymology; D011549:Pseudomonas / Q000201:enzymology / Q000302:isolation & purification; D012231:Rhizobium / Q000201:enzymology
D002863:Chromogenic Compounds; D003470:Culture Media