Characterization of a continuous cell line (MHH-NB-11) derived from advanced neuroblastoma.
A continuous cell line was established from an explanted tumor biopsy obtained from a patient with advanced neuroblastoma, which showed no response to chemotherapy. This cell line MHH-NB-11 retained most properties of the original immature tumor, even after xenotransplantation into nude mice. The cell line consisted of small dense cells with scant cytoplasm and thin; long processes and expressed neuron-specific enolase and synaptophysin, but neither GFAP nor S-100 protein. Karyotyping showed karyograms with 49 to 54 chromosomes, with a modal at 52. Most cells had trisomy 2,7,8,20, but only few structural aberrations were observed. Two of four chromosomes 1 showed a rearrangement of the terminal 1p segment, and all cells had a long HSR on the long arm of one of the chromosomes 13. This region hybridized in situ with the N-myc probe pNB-1. N-myc was amplified 20-fold in this neuroblastoma cell line as determined in Southern blot analysis. This cell line should be a useful tool in vitro or as a xenograft model for neuroblastoma research.
Pietsch
T
T
Abteilung Pädiatrische Hämatologie und Onkologie, Medizinische Hochschule Hannover, Federal Republic of Germany.
Göttert
E
E
Meese
E
E
Blin
N
N
Feickert
H J
HJ
Riehm
H
H
Kovacs
G
G
D000818:Animals; D002460:Cell Line; D002675:Child, Preschool; D002871:Chromosome Banding; D046508:Culture Techniques / Q000379:methods; D003598:Cytoskeletal Proteins / Q000032:analysis; D004273:DNA, Neoplasm / Q000235:genetics; D006801:Humans; D007621:Karyotyping; D008297:Male; D051379:Mice; D008819:Mice, Nude; D009367:Neoplasm Staging; D009368:Neoplasm Transplantation; D009447:Neuroblastoma / Q000235:genetics / Q000473:pathology* / Q000648:ultrastructure; D009693:Nucleic Acid Hybridization; D014183:Transplantation, Heterologous
D003598:Cytoskeletal Proteins; D004273:DNA, Neoplasm