title: A glycoprotein of oviductal origin alters biochemical properties of the zona pellucida of hamster egg.
abstract: Eggs were isolated from ovaries and oviducts of the golden hamster and the components of zonae pellucidae were examined using density gradient SDS-polyacrylamide electrophoresis. Zonae of ovarian eggs (ZP-OVA) had three major components corresponding to the so-called ZP-1, ZP-2, and ZP-3. Zonae of recently ovulated eggs collected from oviducts (ZP-OVI) had a 200-240 K component (ZP-O) in addition to the three components present in ZP-OVA. When ovarian and oviductal eggs were stained with FITC-conjugated B. simplicifolia-1 lectin (BS-1), which specifically binds to alpha-D-galactose- or alpha-N-acetyl-D-galactosamine-like terminal saccharide residues, ZP-OVI was intensely stained, while ZP-OVA was not. ZP-OVA gained the ability to bind to BS-1 after a brief treatment with oviduct extracts. These results suggest that biochemical properties of hamster zonae change after transport of eggs from ovary to the oviduct. The addition of the 200-240 K component of oviductal origin to preexisting zona components seems to be responsible for this change.
authors.affiliation: Bio Science Laboratory and Developmental and Reproductive Biology Center, Yamagata, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Oikawa
authors.forename: Y
authors.initials: Y
authors.lastname: Sendai
authors.forename: S
authors.initials: S
authors.lastname: Kurata
authors.forename: R
authors.initials: R
authors.lastname: Yanagimachi
mesh_terms: D000818:Animals; D006224:Cricetinae; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006023:Glycoproteins / Q000032:analysis*; D008647:Mesocricetus; D010057:Oviducts / Q000032:analysis*; D010063:Ovum / Q000032:analysis*; D015044:Zona Pellucida / Q000032:analysis*
chemical_list: D006023:Glycoproteins