A new technique for lipid analysis using liquid chromatography/mass spectrometry.
It has been previously reported that ethanol alters the level of polyunsaturated fatty acids, and this may be related to alteration of membrane physical properties. Therefore, developing a technique to efficiently analyze lipid molecular species is of value for alcohol research. A new technique which can provide detailed structural information for most major lipid classes has been developed in our laboratory using thermospray liquid chromatography/mass spectrometry (LC/MS). In this technique, on-line LC separation is achieved with a conventional flow rate and the LC effluent is carried into the mass spectrometer via a heated capillary where it is vaporized and ionized. The results thus obtained for the major lipid classes including fast separation methods and quantitative aspects will be presented.
Kim
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Division of Intramural Clinical and Biological Research, National Institute of Alcohol Abuse and Alcoholism, Bethesda, MD 20892.
Salem
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D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D010713:Phosphatidylcholines / Q000032:analysis; D010743:Phospholipids / Q000032:analysis*
D010713:Phosphatidylcholines; D010743:Phospholipids