title: Progesterone assay by inhibition of reverse passive haemagglutination.
abstract: An assay for the steroid hormone progesterone is described based on the inhibition of the agglutination by progesterone-bovine serum albumin conjugate of red cells coupled with monoclonal antiprogesterone antibody (reverse passive haemagglutination). A low-affinity agglutination system produced the optimal sensitivity, capable of detecting less than 1 ng/ml steroid. This assay has the simplicity and sensitivity for a potential clinical test for placental or corpus luteum function. It also serves as a model for detection of other small ligands (drugs, hormones) by inhibition of reverse passive haemagglutination.
authors.affiliation: University Department of Pathology, Addenbrooke's Hospital, Cambridge, UK.
authors.forename: R R
authors.initials: RR
authors.lastname: Coombs
authors.forename: D
authors.initials: D
authors.lastname: Kirk
authors.forename: J
authors.initials: J
authors.lastname: Saunders
authors.forename: R B
authors.initials: RB
authors.lastname: Heap
authors.forename: M J
authors.initials: MJ
authors.lastname: Taussig
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D001667:Binding, Competitive; D006385:Hemagglutination Inhibition Tests / Q000379:methods; D006908:Hydroxyprogesterones / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D011374:Progesterone / Q000032:analysis* / Q000494:pharmacology; D012710:Serum Albumin, Bovine / Q000494:pharmacology
chemical_list: D000911:Antibodies, Monoclonal; D006908:Hydroxyprogesterones; D012710:Serum Albumin, Bovine; D011374:Progesterone; C030250:progesterone 11-hemisuccinate