title: Protein serotyping of Streptococcus pneumoniae based on reactivity to six monoclonal antibodies.
abstract: Six monoclonal antibodies to proteins of Streptococcus pneumoniae were tested in a dot blot assay for reactivity with 499 clinical isolates of pneumococci. Forty-four percent of the isolates reacted with at least one of the antibodies. Nineteen patterns of reactivity were identified and each designated as a provisional protein serotype. Protein serotyping identified pneumococcal strains independently of their capsular type and made it possible to differentiate strains within most capsular types. A protein serotyping system provides a new dimension to the phenotypic identification of S. pneumoniae and may eventually provide a basis for assessing the population structure of these organisms.
authors.affiliation: Department of Microbiology, University of Alabama, Birmingham 35294.
authors.forename: W D
authors.initials: WD
authors.lastname: Waltman
authors.forename: L S
authors.initials: LS
authors.lastname: McDaniel
authors.forename: B
authors.initials: B
authors.lastname: Andersson
authors.forename: L
authors.initials: L
authors.lastname: Bland
authors.forename: B M
authors.initials: BM
authors.lastname: Gray
authors.forename: C S
authors.initials: CS
authors.lastname: Eden
authors.forename: D E
authors.initials: DE
authors.lastname: Briles
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000145:classification; D051379:Mice; D012703:Serotyping / Q000379:methods; D013296:Streptococcus pneumoniae / Q000145:classification*
chemical_list: D000911:Antibodies, Monoclonal; D000954:Antigens, Surface