title: Determination of clebopride in plasma by capillary gas chromatography-negative-ion chemical ionization mass spectrometry.
abstract: A procedure for the analysis of clebopride in plasma using capillary gas chromatography-negative-ion chemical ionization mass spectrometry has been developed. Employing an ethoxy analogue as internal standard, the two compounds were extracted from basified plasma using dichloromethane. Subsequent reaction with heptafluorobutyryl imidazole produced volatile monoheptafluorobutyryl derivatives whose ammonia negative-ion mass spectra proved ideal for selected-ion monitoring. The recovery of clebopride from plasma at 0.536 nmol/l was found to be 85.5 +/- 0.9% (n = 3) whilst measurement down to 0.268 nmol/l was possible with a coefficient of variation of 7.9%. Plasma levels of the compound are reported in two volunteers following ingestion of 1 mg of clebopride as the malate salt.
authors.affiliation: Simbec Research Limited, Merthyr Tydfil, Mid Glamorgan, U.K.
authors.forename: P R
authors.initials: PR
authors.lastname: Robinson
authors.forename: M D
authors.initials: MD
authors.lastname: Jones
authors.forename: J
authors.initials: J
authors.lastname: Maddock
mesh_terms: D000932:Antiemetics / Q000097:blood*; D001549:Benzamides / Q000097:blood*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007202:Indicators and Reagents; D008297:Male
chemical_list: D000932:Antiemetics; D001549:Benzamides; D007202:Indicators and Reagents; C014417:clebopride