Erythroid specific activation of the Xenopus laevis adult alpha-globin promoter in transient heterokaryons.
Insertion of 1.5 kb of the 5' flanking region of the adult alpha-globin gene of X. laevis in front of the CAT structural gene promotes synthesis of CAT in transiently transfected X. laevis kidney cells. Fusion of transiently transfected kidney cells with erythroblasts isolated from anaemic frogs stimulates CAT expression 3-4 fold in the resulting transient heterokaryons. The stimulation is specific for the alpha-globin promoter and is obtained after fusion with erythroid cells but not with hepatocytes or kidney cells. Stably transfected kidney cells express drastically reduced CAT activity as compared with transiently transfected cells. Nevertheless, fusion of stably transfected kidney cells with erythroblasts leads to a 10-17 fold stimulation of CAT expression. The experiments suggest that erythroid specific transacting factors stimulate expression of CAT controlled by the adult alpha-globin promoter.
Stalder
J
J
Abteilung für Zell- und Entwicklungsbiologie, Universität Bern, Switzerland.
D000375:Aging; D000818:Animals; D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase / Q000096:biosynthesis; D004900:Erythroblasts / Q000502:physiology*; D005260:Female; D005786:Gene Expression Regulation; D005914:Globins / Q000235:genetics*; D007668:Kidney / Q000201:enzymology / Q000502:physiology*; D011401:Promoter Regions, Genetic; D014162:Transfection; D014982:Xenopus laevis
D005914:Globins; D015500:Chloramphenicol O-Acetyltransferase