title: Purple-to-blue transition of bacteriorhodopsin in a neutral lipid environment.
abstract: The red shift in the absorption maximum of native purple membrane suspensions caused by deionization is missing in lipid-depleted purple membrane, and the pK of the acid-induced transition is down-shifted to pH approximately 1.4 and has become independent of cation concentration (Szundi, I., and W. Stoeckenius. 1987. Proc. Natl. Acad. Sci. USA. 84:3681-3684). However, the proton pumping function cannot be demonstrated in these membranes. When native acidic lipids of purple membrane are exchanged for egg phosphatidylcholine or digalactosyldiglyceride, bacteriorhodopsin is functionally active in the modified membrane. It shows spectral shifts upon light-dark adaptation, a photocycle with M-intermediate and complex decay kinetics; when reconstituted into vesicles with the same neutral lipids, it pumps protons. Unlike native purple membrane, lipid-substituted modified membranes do not show a shift of the absorption maximum to longer wavelength upon deionization. A partial shift can be induced by titration with HCl; it has a pK near 1.5 and no significant salt dependence. Titration with HNO3 and H2SO4, which causes a complete transition in the lipid-depleted membranes, i.e., it changes their colors from purple to blue, does not cause the complete transition in the lipid-substituted preparations. These results show that the purple color of bacteriorhodopsin is independent of cations and their role in the purple-to-blue transition of native membranes is indirect. The purple and blue colors of bacteriorhodopsin are interpreted as two conformational states of the protein, rather than different protonation states of a counterion to the protonated Schiff base.
authors.affiliation: Department of Biochemistry and Biophysics, University of California, San Francisco 94143-0130.
authors.forename: I
authors.initials: I
authors.lastname: Szundi
authors.forename: W
authors.initials: W
authors.lastname: Stoeckenius
mesh_terms: D001436:Bacteriorhodopsins / Q000378:metabolism*; D038983:Galactolipids; D006017:Glycolipids / Q000494:pharmacology*; D006217:Halobacterium / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010713:Phosphatidylcholines / Q000494:pharmacology*
chemical_list: D038983:Galactolipids; D006017:Glycolipids; D010713:Phosphatidylcholines; C007388:digalactosyldiacylglycerol; D001436:Bacteriorhodopsins