Differentiating the effects of microwave and heat on tissue proteins and their crosslinking by formaldehyde.
Alkaline phosphatase activity in mouse liver blocks, cooled by an ice-bath, decreased by 50% in 5 min of microwave irradiation (280 W). This loss of protein tertiary structure has been mirrored by ultrastructural changes in the same tissue. Microwave irradiation did not produce cleavage or polymerization of lysozyme or haemoglobin. Protein formaldehyde reaction mixtures produced protein polymers between 0 degree and 40 degrees C which could be separated by SDS-polyacrylamide gel electrophoresis. Microwave irradiation of lysozyme or haemoglobin plus formaldehyde on ice-bath up to 30 min produced a similar electrophoretic pattern. When lysozyme or haemoglobin plus formaldehyde was heated to 60 degrees C for 30 min, the protein polymers migrated faster on electrophoresis, suggesting a smaller hydrodynamic volume than expected due to intramolecular crosslink formation, not opened up under the conditions of electrophoresis.
Hopwood
D
D
Department of Pathology, Ninewells Hospital and Medical School, Dundee, UK.
Yeaman
G
G
Milne
G
G
D000469:Alkaline Phosphatase / Q000378:metabolism* / Q000528:radiation effects; D000818:Animals; D003432:Cross-Linking Reagents; D005557:Formaldehyde; D006454:Hemoglobins / Q000032:analysis / Q000528:radiation effects; D006651:Histocytochemistry; D006358:Hot Temperature; D006801:Humans; D008099:Liver / Q000201:enzymology*; D046911:Macromolecular Substances; D051379:Mice; D008872:Microwaves; D008970:Molecular Weight; D009113:Muramidase / Q000378:metabolism* / Q000528:radiation effects; D011506:Proteins / Q000032:analysis* / Q000528:radiation effects
D003432:Cross-Linking Reagents; D006454:Hemoglobins; D046911:Macromolecular Substances; D011506:Proteins; D005557:Formaldehyde; D000469:Alkaline Phosphatase; D009113:Muramidase