title: Cell attachment of human gingival fibroblasts in vitro to porous-surfaced titanium alloy discs coated with collagen and platelet-derived growth factor.
abstract: The influence of biological coating, with or without the incorporation of growth factor, on the migration, attachment and orientation of human gingival fibroblasts in relation to porous-surfaced titanium alloy (Ti6AI4V) discs, was measured. Comparison was made between coating the discs with collagen and with collagen incorporating platelet-derived growth factor (PDGF); controls comprised porous-surfaced discs coated with agar or collagen containing bovine serum albumin (used as a carrier for the PDGF), uncoated porous-surfaced Ti6AI4V discs (with or without additional protein additives) exhibited significantly higher attachment indices (AI) and orientation indices (OI) compared with naked control discs (p less than 0.01); OI was also significantly higher than that of surface-demineralized root slices (p less than 0.001) on days 1, 2 and 3. Addition of PDGF to the collagen resulted in a further enhancement in OI on days 1 and 2 (p less than 0.01) over that shown by discs coated with collagen incorporating the bovine serum albumin vehicle. There was no cell attachment and consequently, no cell orientation, in relation to Ti alloy discs that had been coated with agar. These data suggest that attachment and orientation of cells following migration in relation to porous-surfaced Ti6AI4V discs can be modified by the application of biological molecules to the surface of the disc. This may have a useful application in clinical implantology.
authors.affiliation: MRC Programme in Dental Implantology, Faculty of Dentistry, University of Toronto, Ontario, Canada.
authors.forename: B F
authors.initials: BF
authors.lastname: Lowenberg
authors.forename: R M
authors.initials: RM
authors.lastname: Pilliar
authors.forename: J E
authors.initials: JE
authors.lastname: Aubin
authors.forename: J
authors.initials: J
authors.lastname: Sodek
authors.forename: A H
authors.initials: AH
authors.lastname: Melcher
mesh_terms: D000497:Alloys; D001672:Biocompatible Materials; D002448:Cell Adhesion / Q000187:drug effects; D002465:Cell Movement; D003094:Collagen / Q000494:pharmacology; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure; D005881:Gingiva / Q000166:cytology* / Q000648:ultrastructure; D006801:Humans; D066298:In Vitro Techniques; D008855:Microscopy, Electron, Scanning; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D014025:Titanium
chemical_list: D000497:Alloys; D001672:Biocompatible Materials; D010982:Platelet-Derived Growth Factor; C031462:titanium alloy (TiAl6V4); D003094:Collagen; D014025:Titanium