Colocalization of GABA and glycine immunoreactivities in a subset of retinal neurons in tiger salamander.
Alternate serial 1 micron Durcupan resin sections of tiger salamander retina were stained with antisera against GABA and glycine using postembed immunocytochemical techniques. Although the vast majority of neurons were labeled by either GABA or glycine antiserum, a small percentage of presumed amacrine cells in the inner nuclear layer and cells in the ganglion cell layer were clearly labeled by both antisera, indicative of colocalization of endogenous GABA and glycine. Although there is a greater than 90% chance that a labeled cell will be clearly labeled for either GABA or glycine immunoreactivity, the possibility for cotransmission of two inhibitory transmitters must be considered for a small percentage of these retinal neurons.
Yazulla
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Department of Neurobiology and Behavior, State University of New York, Stony Brook 11794.
Yang
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D000818:Animals; D005998:Glycine / Q000378:metabolism*; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D009474:Neurons / Q000378:metabolism; D012160:Retina / Q000166:cytology / Q000378:metabolism*; D012165:Retinal Ganglion Cells / Q000166:cytology / Q000378:metabolism; D014562:Caudata / Q000378:metabolism*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D005680:gamma-Aminobutyric Acid; D005998:Glycine