Sialic acids in human lymphocytes. Qualitative and quantitative alterations in cancer cases.
Sialic acids, hydrolyzed from human lymphocytes, were determined, in the nanomole range, with a modified form of the periodic acid-thiobarbituric acid assay and liquid chromatography. The l.c. separations were carried out with two different systems, firstly an Aminex HPX-72 S anion-exchange resin and a 0.15M ammonium sulfate mobile phase, and secondly an amine phase (5 microns) and an acetonitrile-phosphate buffer as mobile phase. The lymphocytes of cancer-stricken persons showed an evident rise of the sialic acid content, combined with a shift of the sialic acid distribution to higher O-acetylated derivatives, as compared to the controls.
Holzhauser
R
R
Biochemie Universität des Saarlandes, Saarbrücken, Federal Republic of Germany.
Faillard
H
H
D001943:Breast Neoplasms / Q000097:blood*; D002853:Chromatography, Liquid; D003124:Colorimetry; D006801:Humans; D008214:Lymphocytes / Q000032:analysis*; D008545:Melanoma / Q000097:blood*; D012794:Sialic Acids / Q000097:blood*
D012794:Sialic Acids