In vivo generation of R68.45-pPGH1 hybrid plasmids conferring a Phl+ (meta pathway) phenotype.
Plasmid pPGH1 originating from Pseudomonas putida strain H carries all the genes required for the degradation of phenol (or cresols) via the meta cleavage pathway. Besides mobilization of pPGH1 by a plasmid of the incompatibility group P-1, hybrid plasmids conferring the Phl+ phenotype could be selected, when R68.45 was the conjugative plasmid. The hybrids contain the complete R68.45 and part of pPGH1. Integration of Phl-DNA of pPGH1 into R68.45 occurred exclusively via the IS21 region of R68.45.
Herrmann
H
H
Sektion Biologie, Ernst-Moritz-Arndt-Universität, DDR, Greifswald.
Janke
D
D
Krejsa
S
S
Roy
M
M
D003001:Cloning, Molecular; D003227:Conjugation, Genetic; D003408:Cresols / Q000378:metabolism; D019800:Phenol; D010636:Phenols / Q000378:metabolism*; D010641:Phenotype; D010957:Plasmids; D011549:Pseudomonas / Q000235:genetics*
D003408:Cresols; D010636:Phenols; D019800:Phenol