A domain model for eukaryotic DNA organization: a molecular basis for cell differentiation and chromosome evolution.
A model for eukaryotic chromatin organization is presented in which the basic structural and functional unit is the DNA domain. This simple model predicts that both chromosome replication and cell type-specific control of gene expression depend on a combination of stable and dynamic DNA-nuclear matrix interactions. The model suggests that in eukaryotes, DNA regulatory processes are controlled mainly by the intranuclear compartmentalization of the specific DNA sequences, and that control of gene expression involves multiple steps of specific DNA-nuclear matrix interactions. Predictions of the model are tested using available biochemical, molecular and cell biological data. In addition, the domain model is discussed as a simple molecular mechanism to explain cell differentiation in multi-cellular organisms and to explain the evolution of eukaryotic genomes consisting mainly of repetitive sequences and "junk" DNA.
Bodnar
J W
JW
Department of Biology, Northeastern University, Boston MA 02115.
D000818:Animals; D002454:Cell Differentiation; D002468:Cell Physiological Phenomena; D002843:Chromatin / Q000502:physiology; D004247:DNA / Q000502:physiology*; D004261:DNA Replication; D005057:Eukaryotic Cells / Q000502:physiology*; D005786:Gene Expression Regulation; D006801:Humans; D008957:Models, Genetic
D002843:Chromatin; D004247:DNA