Polymorphism of the seventh component of complement (C7) in a healthy Caucasian population: an immunoblotting study with neuraminidase-treated samples.
Genetic polymorphism of the seventh component of complement (C7) was studied in a healthy Caucasian population using polyacrylamide gel isoelectric focusing of neuraminidase-treated plasma samples and an immunoblotting procedure for the specific detection of C7. Among 248 blood donors, three C7-3/1 heterozygotes were identified, resulting in a C7*3 allele frequency of 0.0061 +/- 0.0035. Neuraminidase treatment of serum or plasma samples is necessary for unequivocal identification of C7*3, which is known to be a hypomorphic variant. This observation is discussed with special reference to previous studies on C7 polymorphism in Caucasian populations, where untreated samples have been used for C7 typing.
Dewald
G
G
Institute of Human Genetics, University of Bonn, FRG.
D015153:Blotting, Western; D003184:Complement C7 / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007526:Isoelectric Point; D008970:Molecular Weight; D009439:Neuraminidase / Q000494:pharmacology; D011110:Polymorphism, Genetic; D044465:White People
D003184:Complement C7; D009439:Neuraminidase