2-D gel comparison of membrane proteins from freshly isolated and cultured fetal and adult hepatocytes.
Labelled membrane proteins from freshly isolated or over-night cultured rat hepatocytes were compared using 2-D gel electrophoresis. The membrane protein patterns changed rapidly after culturing, some changes being apparent after 5 hours in culture. These changes included the disappearance of a number of membrane proteins and the apparent altered glycosylation of others. Stability of hepatocyte membrane proteins in culture did not appear to be related to the stage of hepatocyte differentiation. A recognition that rapid changes in the membrane protein composition may occur after culturing is important for those wishing to use cultured cells to study membrane-associated functions.
Radford
J
J
Pathology Department, University of Melbourne, Parkville, Victoria, Australia.
D000375:Aging; D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D015180:Electrophoresis, Gel, Two-Dimensional; D005333:Fetus / Q000032:analysis / Q000166:cytology; D007457:Iodine Radioisotopes; D008099:Liver / Q000032:analysis / Q000166:cytology* / Q000196:embryology; D008560:Membrane Fluidity; D008565:Membrane Proteins / Q000032:analysis*; D051381:Rats
D007457:Iodine Radioisotopes; D008565:Membrane Proteins