title: Simultaneous quantitation of buspirone and 1-(2-pyrimidinyl)piperazine in human plasma and urine by capillary gas chromatography-mass spectrometry.
abstract: Buspirone and a buspirone metabolite, 1-(2-pyrimidinyl)piperazine (1-PP), are extracted from matrix using C18 extraction columns. The metabolite and its internal standard (d4-1-PP) are derivatized with pentafluorobenzoyl chloride to the corresponding amides. The 1-PP derivatives, buspirone and the buspirone internal standard (5-fluorobuspirone) are co-chromatographed. Chromatography and detection are performed using capillary gas chromatography with a fused-silica column and selected-ion monitoring-mass spectrometry. Linear range of the standard curves in plasma is 0.1-14 ng/ml for buspirone and 0.2-25 ng/ml for 1-PP with lower limits of quantitation of 0.1 and 0.2 ng/ml, respectively. In urine the linear range of the standard curves is 0.2-14 ng/ml for buspirone and 8-500 ng/ml for 1-PP with lower limits of quantitation of 0.2 and 8.0 ng/ml, respectively. Intra-assay accuracies were within 14% for buspirone and 1-PP in plasma and urine. Intra-assay precision was within 12% for both compounds in both matrices.
authors.affiliation: Department of Metabolism and Pharmackinetics, Bristol-Myers Company, Syracuse, NY 13221.
authors.forename: M A
authors.initials: MA
authors.lastname: Sciacca
authors.forename: G F
authors.initials: GF
authors.lastname: Duncan
authors.forename: J P
authors.initials: JP
authors.lastname: Shea
authors.forename: H C
authors.initials: HC
authors.lastname: Faulkner
authors.forename: R H
authors.initials: RH
authors.lastname: Farmen
authors.forename: K A
authors.initials: KA
authors.lastname: Pittman
mesh_terms: D002065:Buspirone / Q000031:analogs & derivatives* / Q000032:analysis* / Q000097:blood / Q000652:urine; D004350:Drug Residues / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007202:Indicators and Reagents; D013607:Tablets
chemical_list: D007202:Indicators and Reagents; D013607:Tablets; C037921:1-(2-pyrimidinyl)piperazine; D002065:Buspirone