[The patterns of colchicine-induced death of lymphoid cells].
It is shown that colchicine injection at doses higher than 1 mg/kg of animal weight induces cell death in thymus, spleen, bone marrow and intestine mucosa. The cell death is accompanied by a regular internucleosomal cleavage of nuclear DNA and by the elimination of the formed fragments from cells. Both the processes begin after a 1.5 hour lag-period and proceed before the outer membrane permeability for supravital dyes increases. DNA degradation is prevented by the inhibitor of protein synthesis cycloheximide. Cytochalasin B does not induce chromatin degradation or cell death and has no effect on radiation death of lymphocytes. A possible role of microtubule destruction as a switch-on mechanism of DNA degradation and cell death is discussed.
Afanas'ev
V N
VN
Korol'
B A
BA
Matylevich
N P
NP
Pechatnikov
V A
VA
Umanskiĭ
S R
SR
D000818:Animals; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D002843:Chromatin / Q000187:drug effects / Q000528:radiation effects; D003078:Colchicine / Q000633:toxicity*; D003513:Cycloheximide / Q000633:toxicity; D003571:Cytochalasin B / Q000633:toxicity; D003599:Cytoskeleton / Q000187:drug effects / Q000528:radiation effects; D004247:DNA / Q000187:drug effects / Q000528:radiation effects; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D005720:Gamma Rays; D008214:Lymphocytes / Q000187:drug effects* / Q000528:radiation effects / Q000648:ultrastructure; D008297:Male; D051379:Mice; D051381:Rats; D011919:Rats, Inbred Strains
D002843:Chromatin; D003571:Cytochalasin B; D004247:DNA; D003513:Cycloheximide; D003078:Colchicine
Zakonomernosti indutsirovannoĭ kolkhitsinom gibeli limfoidnykh kletok.