An enzyme-linked lectin assay for the study of lectin receptors of Leishmania.
An enzyme-linked lectin assay (ELLA) based on the ELISA assay, using intact formalin-fixed promastigotes to coat poly-L-lysine-treated microtiter plates is described. The assay was used to study the lectin receptors of Leishmania donovani chagasi, L. donovani donovani and L. mexicana amazonensis. ConA, RCA, WGA, and PNA receptors were found in the three parasites. SBA receptors were found to be as frequent as the other receptors in L. donovani chagasi but not in the other two parasites which showed little SBA binding. Trypsin treatment of the two L. donovani subspecies did not remove any of the lectin receptors studied.
Andrade
P P
PP
Departamento de Biofísica e Radiobiologia, Universidade Federal de Pernambuco, Recife, Brasil.
Schottelius
J
J
Andrade
C R
CR
D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006023:Glycoproteins / Q000032:analysis; D007893:Leishmania donovani / Q000032:analysis*; D007894:Leishmania mexicana / Q000032:analysis*; D011975:Receptors, Mitogen / Q000032:analysis*; D014357:Trypsin / Q000494:pharmacology
D006023:Glycoproteins; D011975:Receptors, Mitogen; D014357:Trypsin