The poly(U) translational capacity of Fischer 344 rat liver does not deteriorate with age and is not affected by dietary regime.
The translational capacity of ribosomes isolated from the livers of young (60-170 days, n = 13) and old (26.5 months, n = 11) Fischer 344 rats was assessed by poly(U)-directed [3H]phenylalanine incorporation. Run-off ribosomes and S100 supernatant were separately prepared from each individual liver. Ribosome concentrations were carefully measured. Individual differences were seen among young rats and among old rats; however, when considered as a group, the old rats, whether diet-restricted or fed ad libitum, translated poly(U) as well, or almost as well, as the young ones.
Laughrea
M
M
Lady Davis Institute for Medical Research, Sir Mortimer B. Davis Jewish General Hospital, Montréal, Québec, Canada.
Latulippe
J
J
D000375:Aging / Q000378:metabolism*; D000818:Animals; D004032:Diet; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism*; D008297:Male; D010649:Phenylalanine / Q000378:metabolism; D011072:Poly U / Q000235:genetics*; D014176:Protein Biosynthesis; D051381:Rats; D011916:Rats, Inbred F344; D012270:Ribosomes / Q000378:metabolism
D011072:Poly U; D010649:Phenylalanine