title: Deterioration of membrane morphology, phospholipids, and cytoskeletal protein in rat erythrocytes exposed to tert-butyl hydroperoxide: protection by exogenous glutathione fails in selenium deficiency.
abstract: Here we report scanning electron microscopy, phospholipid and fatty acid composition and cytoskeleton proteins of erythrocytes from Se-adequate and Se-deficient rats, showing protection by glutathione (GSH) against tert-butyl hydroperoxide (t-BuOOH)-induced hemolysis of erythrocytes from Se-adequate, but not from Se-deficient rats. Without exogenous GSH, erythrocytes incubated with t-BuOOH exhibited remarkable deterioration of cell membranes with diminished membrane phosphatidyl-ethanolamine, -serine, and -inositol and selective loss of a cytoskeletal protein, ankyrin 2-1. Without exogenous GSH these changes occurred in erythrocytes from both Se-deficient and Se-adequate rats. Dietary Se, through provision of glutathione peroxidase (GSH-Px) in erythrocytes as a probable scavenger of t-BuOOH, protects against hemolysis when GSH is available.
authors.affiliation: Research Institute for Food Science, Kyoto University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Suzuki
authors.forename: C H
authors.initials: CH
authors.lastname: Kim
authors.forename: K
authors.initials: K
authors.lastname: Yasumoto
mesh_terms: D000818:Animals; D003598:Cytoskeletal Proteins / Q000097:blood*; D004591:Electrophoresis, Polyacrylamide Gel; D004910:Erythrocyte Membrane / Q000378:metabolism / Q000648:ultrastructure*; D005227:Fatty Acids / Q000032:analysis; D005978:Glutathione / Q000494:pharmacology*; D008297:Male; D008563:Membrane Lipids / Q000097:blood*; D010545:Peroxides / Q000494:pharmacology*; D010743:Phospholipids / Q000097:blood*; D051381:Rats; D011919:Rats, Inbred Strains; D012643:Selenium / Q000172:deficiency*; D020122:tert-Butylhydroperoxide
chemical_list: D003598:Cytoskeletal Proteins; D005227:Fatty Acids; D008563:Membrane Lipids; D010545:Peroxides; D010743:Phospholipids; D020122:tert-Butylhydroperoxide; D005978:Glutathione; D012643:Selenium