title: Prenatal diagnosis with biotinylated chromosome specific probes.
abstract: We have used a Y-chromosome specific DNA probe in a controlled study to determine the presence of Y-chromosome material and to detect numerical abnormalities in uncultured amniotic fluid cells by fluorescent hybridization. Using this non-radioactive method, we correctly predicted fetal sex within 48 h in all but 3 of 54 cases and identified an XYY syndrome. The technique was previously tested with no false-positive or false-negative results on cultured interphase or metaphase nuclei of fetal fibroblasts and adult T-lymphocytes. Fluorescent in situ hybridization was applied to long-term fixed cytogenetic preparations up to 44 months old and was shown to be reliable.
authors.affiliation: Centre de Diagnostic Prenatal et de Foetologie, Institut de Puériculture, Paris, France.
authors.forename: B
authors.initials: B
authors.lastname: Guyot
authors.forename: A
authors.initials: A
authors.lastname: Bazin
authors.forename: Y
authors.initials: Y
authors.lastname: Sole
authors.forename: C
authors.initials: C
authors.lastname: Julien
authors.forename: F
authors.initials: F
authors.lastname: Daffos
authors.forename: F
authors.initials: F
authors.lastname: Forestier
mesh_terms: D000649:Amniocentesis / Q000379:methods*; D001710:Biotin; D015342:DNA Probes; D005260:Female; D005456:Fluorescent Dyes; D006801:Humans; D009693:Nucleic Acid Hybridization; D011247:Pregnancy; D011262:Pregnancy Trimester, Second; D012732:Sex Determination Analysis
chemical_list: D015342:DNA Probes; D005456:Fluorescent Dyes; D001710:Biotin