title: A method for parallel determination of choline acetyltransferase and muscarinic cholinergic receptors: application in aged-rat brain.
abstract: We have devised a method for the parallel determination of choline acetyltransferase (CAT) and muscarinic cholinergic receptor (mCh-R) in the same brain tissue. The method for CAT activity determination is more rapid, simplified, stable, and economical than the usual Fonnum's method. With our method, age-associated changes in CAT activity and mCh-R levels were examined. Although CAT activity hardly changed with age except in a few areas, mCh-R binding of aged-rats was markedly reduced in all areas. These results suggest that the change in mCh-R represents an age-associated biochemical change in the brain and that determination of CAT activity is not sufficient for the study of age-associated changes in the brain cholinergic system.
authors.affiliation: Department of Neurochemistry, Okayama University Medical School, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Haba
authors.forename: N
authors.initials: N
authors.lastname: Ogawa
authors.forename: M
authors.initials: M
authors.lastname: Kawata
authors.forename: A
authors.initials: A
authors.lastname: Mori
mesh_terms: D000375:Aging / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000254:growth & development / Q000378:metabolism*; D002795:Choline O-Acetyltransferase / Q000378:metabolism*; D008297:Male; D009448:Neurochemistry / Q000379:methods*; D051381:Rats; D011919:Rats, Inbred Strains; D011976:Receptors, Muscarinic / Q000378:metabolism*
chemical_list: D011976:Receptors, Muscarinic; D002795:Choline O-Acetyltransferase