Increase of dentin phosphophoryn with dentin formation.
Dentin phosphophoryn was quantified on bovine and rabbit dentin at three developmental stages. Phosphophoryn was extracted from teeth with 0.6M HCl, and quantified as optical density on DEAE-cellulose chromatogram or as phosphoserine content. Bovine phosphophoryn showed progressive increase with formation of dentin. Matrix-associated phosphophoryn was also quantified as phosphoserine content in insoluble dentin residue which was extracted with 6 M urea after decalcification. This fraction increased with formation of dentin both in bovine and rabbit dentin. Phosphophoryn is thought to be related to the later stage of dentin formation.
Fujisawa
R
R
Department of Biochemistry, School of Dentistry, Hokkaido University, Sapporo, Japan.
Kuboki
Y
Y
D000818:Animals; D002417:Cattle; D002848:Chromatography, DEAE-Cellulose; D003810:Dentinogenesis; D004591:Electrophoresis, Polyacrylamide Gel; D007180:Incisor / Q000032:analysis; D010750:Phosphoproteins / Q000032:analysis*; D011817:Rabbits; D012967:Sodium Dodecyl Sulfate
D010750:Phosphoproteins; C017630:phosphophoryn; D012967:Sodium Dodecyl Sulfate