Elasmobranch tissue culture: in vitro growth of brain explants from a shark (Rhizoprionodon) and dogfish (Squalus).
Brain explants from a Caribbean sharpnose shark (Rhizoprionodon porosus) and six spiny dogfish (Squalus acanthias) were cultured at 22 degrees C in Leibovitz L-15 medium, 1000 mOsmol, with 350 mM urea, 20 mM added NaCl, and either 2% fetal bovine serum, 2% bull shark (Carcharhinus leucas) serum, or 2% spiny dogfish serum. Cell outgrowth from explants appeared after 1 month. Cells were subcultured once with 20% survival, and were viable at termination of the trials 6 months later. Cell morphology appeared normal throughout the experiment.
Garner
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Biomedical Research Division, Mote Marine Laboratory, Sarasota, Florida 33577.
D000818:Animals; D001921:Brain / Q000166:cytology*; D002478:Cells, Cultured; D004284:Dogfish / Q000033:anatomy & histology*; D012754:Sharks / Q000033:anatomy & histology*