Localization of vasoactive intestinal peptide and peptide histidine isoleucine immunoreactivity and mRNA within the rat suprachiasmatic nucleus.
The distribution of vasoactive intestinal peptide (VIP) and peptide histidine isoleucine (PHI) mRNA within the suprachiasmatic nucleus (SCN) of rats was evaluated by immunocytochemistry and in situ hybridization. The pattern of VIP and PHI immunoreactivity corresponded closely to the distribution of VIP/PHI mRNA within the ventrolateral SCN. Clear hybridization signal was observed within the SCN of rats killed 5 h after light onset and in rats killed 2 h after the onset of the dark phase of the light-dark cycle. Visual examination of the grain density within the autoradiographs suggested that VIP/PHI mRNA may occur in higher concentrations shortly after the onset of darkness than 5 h after the onset of the light phase.
Stopa
E G
EG
Department of Pathology, Tufts University School of Medicine, Boston, MA.
Minamitani
N
N
Jonassen
J A
JA
King
J C
JC
Wolfe
H
H
Mobtaker
H
H
Albers
H E
HE
D000818:Animals; D001345:Autoradiography; D007150:Immunohistochemistry; D008297:Male; D009693:Nucleic Acid Hybridization; D010451:Peptide PHI / Q000032:analysis / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics*; D051381:Rats; D013462:Sulfur Radioisotopes; D013493:Suprachiasmatic Nucleus / Q000166:cytology / Q000378:metabolism*; D014158:Transcription, Genetic; D014660:Vasoactive Intestinal Peptide / Q000235:genetics*
D010451:Peptide PHI; D012333:RNA, Messenger; D013462:Sulfur Radioisotopes; D014660:Vasoactive Intestinal Peptide