Purification of specific heterologous F(ab)2 fragments with DEAE-Zeta-Prep cartridges for ion-exchange chromatography.
A simple two-step procedure for purifying F(ab)2 fragments of horse immunoglobulins is described. In the first step, the horse plasma is diluted, made up to 12% (w/v) with ammonium sulphate and digested with pepsin. In the second step, the previously dialyzed solution is chromatographed. Instead of a normal ion-exchange resin, a DEAE-cellulose, covalently linked to a synthetic vinyl polymer, was used (DEAE-Zeta-Prep). With this assembly it is possible to perform chromatography at a high flow-rate without the problems related to the use of large columns. The yield and purity of the final product are satisfactory. This method has been scaled up for industrial application.
Benanchi
P L
PL
Centro Sviluppo Biotecnologie, Siena, Italy.
Gazzei
G
G
Giannozzi
A
A
D000818:Animals; D002848:Chromatography, DEAE-Cellulose; D006736:Horses; D007140:Immunoglobulin Fab Fragments / Q000302:isolation & purification*
D007140:Immunoglobulin Fab Fragments