[Occurrence and determination of okadaic acids in mussels from the German North Sea coast].
A method for the determination of okadaic acid, a typical DSP toxin, in mussels is described. The homogenized hepatopancreas is extracted and the crude extract purified by liquid/liquid partition chromatography. The derivatization of okadaic acid to 9-anthrylmethyl-okadaic acid (9-AM-OA) is achieved using the derivatization reagent 9-anthryldiazomethane (ADAM) and the clean-up is carried out by SPE with silica gel. 9-AM-OA is determined by HPLC using a RP-18 column with CH3CN/THF/H2O as the eluent. The selective and sensitive detection of 9-AM-OA is performed by coupling UV and fluorimetric detectors (detection limit: 0.5 microgram okadaic acid/g hepatopancreas).
Luckas
B
B
Lebensmittel- und Veterinäruntersuchungsamt des Landes Schleswig-Holstein, Aussenstelle Flensburg, Bundesrepublik Deutschland.
Meixner
R
R
D000818:Animals; D001427:Bacterial Toxins; D049872:Bivalvia / Q000032:analysis*; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D000087522:Cyanobacteria Toxins; D004988:Ethers, Cyclic / Q000032:analysis*; D056890:Eukaryota; D005506:Food Contamination; D008387:Marine Toxins / Q000032:analysis*; D052998:Microcystins; D019319:Okadaic Acid; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
D001427:Bacterial Toxins; D000087522:Cyanobacteria Toxins; D004988:Ethers, Cyclic; D008387:Marine Toxins; D052998:Microcystins; D019319:Okadaic Acid
Vorkommen und Bestimmung von Okadasäure in Muscheln der deutschen Nordseeküste.