Separation of Akazara scallop and rabbit troponin components by a single-step chromatography on CM-Toyopearl.
Three components of Akazara scallop (Chlamys nipponensis akazara) troponin were well separated from each other by a single-step chromatography on CM-Toyopearl, although they were hardly separated on DEAE-Sephadex A-25. Moreover, by means of this CM-chromatography, the troponin components of rabbit were also readily separated with high purities and in high yields. The components thus separated were readily reconstituted and the Ca2+ regulatory function was fully recovered.
Ojima
T
T
Department of Chemistry, Faculty of Fisheries, Hokkaido University.
Nishita
K
K
D000818:Animals; D002852:Chromatography, Ion Exchange / Q000379:methods; D008974:Mollusca / Q000378:metabolism*; D009132:Muscles / Q000378:metabolism*; D011108:Polymers; D011817:Rabbits; D014336:Troponin / Q000302:isolation & purification*; D014508:Urea
D011108:Polymers; D014336:Troponin; C058606:Toyopearl 650; D014508:Urea