title: Hypertonic aerosol inhalation does not alter central airway blood flow in dogs.
abstract: Tracheobronchial blood flow in dogs increases with cold or dry air hyperventilation, possibly as a result of airway drying leading to increased osmolarity of airway surface fluid. This study was designed to examine whether administration of aerosols of various tonicity to alter airway surface fluid osmolarity would induce similar blood flow changes. Tracheobronchial blood flow was measured by the radioactive microsphere technique in six anesthetized dogs ventilated with warm humid air (100% relative humidity) for 15 min (period 1), air containing ultrasonically nebulized saline aerosol (1,711 mosmol/kg) for 3 min (period 2) and 12 min (period 3), and the same aerosol at a higher nebulizer output for a further 3 min (period 4). Between periods 3 and 4, the dogs were ventilated with warm humid air for 30 min to reestablish base-line conditions. In another five dogs, measurements were made after 30 min of ventilation with 1) warm humid air, 2) isotonic saline aerosol, 3) warm humid air, 4) distilled water aerosol (3 dogs), and hypertonic saline aerosol (2 dogs). After the last measurement was made, each dog was killed, the trachea and major bronchi were excised, and blood flow was calculated. No change in blood flow was found during any period of aerosol inhalation. The osmolar load imposed on the airways was estimated and was similar to that occurring during cold or dry air hyperventilation. These data suggest that increasing osmolarity of airway surface fluid does not explain the blood flow changes seen during hyperventilation of cold or dry air.
authors.affiliation: University of British Columbia Pulmonary Research Laboratory, St. Paul's Hospital, Vancouver, Canada.
authors.forename: D J
authors.initials: DJ
authors.lastname: Godden
authors.forename: E M
authors.initials: EM
authors.lastname: Baile
authors.forename: M
authors.initials: M
authors.lastname: Okazawa
authors.forename: P D
authors.initials: PD
authors.lastname: Paré
mesh_terms: D000336:Aerosols; D000818:Animals; D001783:Blood Flow Velocity; D001980:Bronchi / Q000098:blood supply; D004285:Dogs; D006985:Hyperventilation / Q000503:physiopathology; D009994:Osmolar Concentration; D012137:Respiratory System / Q000098:blood supply*; D012462:Saline Solution, Hypertonic; D014132:Trachea / Q000098:blood supply
chemical_list: D000336:Aerosols; D012462:Saline Solution, Hypertonic