title: Effect of heroin-conditioned auditory stimuli on cerebral functional activity in rats.
abstract: Cerebral functional activity was measured as changes in distribution of the free fatty acid [1-14C]octanoate in autoradiograms obtained from rats during brief presentation of a tone previously paired to infusions of heroin or saline. Rats were trained in groups of three consisting of one heroin self-administering animal and two animals receiving yoked infusions of heroin or saline. Behavioral experiments in separate groups of rats demonstrated that these training parameters imparts secondary reinforcing properties to the tone for animals self-administering heroin while the tone remains behaviorally neutral in yoked-infusion animals. The optical densities of thirty-seven brain regions were normalized to a relative index for comparisons between groups. Previous pairing of the tone to heroin infusions irrespective of behavior (yoked-heroin vs. yoked-saline groups) produced functional activity changes in fifteen brain areas. In addition, nineteen regional differences in octanoate labeling density were evident when comparison was made between animals previously trained to self-administer heroin to those receiving yoked-heroin infusions, while twelve differences were noted when comparisons were made between the yoked vehicle and self administration group. These functional activity changes are presumed related to the secondary reinforcing capacity of the tone acquired by association with heroin, and may identify neural substrates involved in auditory signalled conditioning of positive reinforcement to opiates.
authors.affiliation: Department of Biology, Marquette University, Milwaukee, WI 53233.
authors.forename: T C
authors.initials: TC
authors.lastname: Trusk
authors.forename: E A
authors.initials: EA
authors.lastname: Stein
mesh_terms: D000161:Acoustic Stimulation; D000818:Animals; D001345:Autoradiography; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D002210:Caprylates / Q000378:metabolism; D002250:Carbon Radioisotopes; D003216:Conditioning, Operant / Q000187:drug effects*; D003932:Heroin / Q000494:pharmacology*; D008297:Male; D009928:Organ Specificity; D051381:Rats; D011919:Rats, Inbred Strains; D012054:Reinforcement, Psychology; D012646:Self Administration
chemical_list: D002210:Caprylates; D002250:Carbon Radioisotopes; D003932:Heroin; C031492:octanoic acid