Activated adherent large granular lymphocytes/natural killer (LGL/NK) cells change their migratory behaviour.
Unstimulated large granular lymphocytes/natural killer (LGL/NK) cells, unlike small T lymphocytes, exhibit prompt locomotion into nitrocellulose filters in response to chemo-attactrants, but, unlike monocytes, are unable to migrate as adherent cells across polycarbonate filters. Upon activation with 4-B-phorbol 12,13 dibutyrate (PDBU), LGL/NK cells become adherent and change their migratory behaviour, having the ability to migrate as adherent cells across polycarbonate filters. PDBU-treated high-density T lymphocytes did not show, under the same conditions, locomotory activity. The change in migratory behaviour following activation may represent an important determinant of the ability of activated LGL/NK cells to adhere to vascular linings and localize in tissues.
Pirelli
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Laboratory of Human Immunology, Istituto di Ricerche Farmacologiche Mario Negri, Milan, Italy.
Allavena
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Mantovani
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D002465:Cell Movement; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007694:Killer Cells, Natural / Q000502:physiology*; D008213:Lymphocyte Activation; D015240:Phorbol 12,13-Dibutyrate / Q000494:pharmacology
D015240:Phorbol 12,13-Dibutyrate