The acidification response of normal subjects to ammonium chloride using a 3-day loading test.
The acidification response to NH4 Cl loading (0.1 g/kg bw/day) was tested in 16 normal healthy subjects in the basal fasting state on Day 4, the subjects having taken the salt daily for the 3 previous days. The response was measured in terms of blood pH and in urine, creatinine, phosphate, pH, titratable acidity, ammonium, net acidity and creatinine clearance. To minimise inter-subject variation the urine values were adjusted to a standard body surface area of 1.73 m2. A normal range for the blood pH of the mean value +/- 2 SD, encompassed the observed range of values. However, to fit the observed range of acid-base values in urine into the 2 SD range required a logarithmic transformation of the data. Statistical analysis confirmed a significant correlation between blood [H+] net acid secretion, urine titratable acidity and ammonium. Urine net acid secretion was positively correlated with urinary phosphate, titratable acidity and ammonium.
Fry
I D
ID
Department of Chemical Pathology, Frimley Park Hospital, Camberley, Surrey, UK.
Cusick
P E
PE
Alston
W C
WC
Montgomery
B
B
Tovey
J E
JE
D000136:Acid-Base Equilibrium; D000328:Adult; D000367:Age Factors; D000643:Ammonium Chloride / Q000008:administration & dosage / Q000494:pharmacology*; D001769:Blood; D001830:Body Surface Area; D004305:Dose-Response Relationship, Drug; D004334:Drug Administration Schedule; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008297:Male; D008875:Middle Aged; D012737:Sex Factors; D014556:Urine / Q000032:analysis
D000643:Ammonium Chloride