title: Protective effects of vitamins E and C on erythrocytes in blood preserved in ACD solution and stored at 4 degrees C.
abstract: Vitamins E and C were used for the enrichment of the fluid for blood preservation. Whole blood with added ACD solution and vitamins E and C was stored at 4 degrees C for 21 days. The performed determinations included: haematocrit, osmotic fragility of the erythrocytes, ATP and 2,3-DPG content in the erythrocytes, and echinocyte count. A protective effect of these vitamins on erythrocytes was manifested by the decreased osmotic fragility, the steep fall of 2,3-DPG compared with the blood preserved in the vitamin-free ACD solution.
authors.affiliation: Department of Physiopathology, Institute of Haematology, Warsaw, Poland.
authors.forename: J
authors.initials: J
authors.lastname: Kopeć-Szlezak
authors.forename: M
authors.initials: M
authors.lastname: Grabarczyk
authors.forename: I
authors.initials: I
authors.lastname: Szczepańska
authors.forename: S
authors.initials: S
authors.lastname: Furman
mesh_terms: D019794:2,3-Diphosphoglycerate; D000255:Adenosine Triphosphate / Q000097:blood; D001205:Ascorbic Acid / Q000494:pharmacology*; D001772:Blood Cell Count; D001793:Blood Preservation / Q000379:methods*; D004163:Diphosphoglyceric Acids / Q000097:blood; D004912:Erythrocytes / Q000187:drug effects*; D005615:Freezing; D006400:Hematocrit; D006801:Humans; D009996:Osmotic Fragility / Q000187:drug effects; D014810:Vitamin E / Q000494:pharmacology*
chemical_list: D004163:Diphosphoglyceric Acids; D019794:2,3-Diphosphoglycerate; D014810:Vitamin E; D000255:Adenosine Triphosphate; D001205:Ascorbic Acid