A simple spectrophotometric assay for urinary leukocyte esterase activity.
We have developed a sensitive spectrophotometric method for assaying urinary leukocyte esterase activity by employing a synthetic substrate, N-toluene sulfonyl indoxyl alanine ester. This kinetic assay can be performed with a small aliquot of urine, by following the change in absorbance of the chromophore at 385 nm. It is rapid and specific for leukocyte esterase and therefore can be used in the early diagnosis of urinary tract infection.
Murthy
V V
VV
Department of Laboratory Medicine, Albert Einstein College of Medicine, Bronx, New York 10461.
Karmen
A
A
D002265:Carboxylic Ester Hydrolases / Q000652:urine*; D004950:Esterases / Q000097:blood* / Q000652:urine; D006801:Humans; D007202:Indicators and Reagents; D007700:Kinetics; D007962:Leukocytes / Q000201:enzymology*; D013053:Spectrophotometry / Q000379:methods; D014556:Urine / Q000166:cytology*
D007202:Indicators and Reagents; D004950:Esterases; D002265:Carboxylic Ester Hydrolases