In vivo cytogenetic studies on mice exposed to Orange G, a food colourant.
Orange G, a monoazo dye, used as a food colourant, was evaluated with in vivo cytogenetic assays to determine its genotoxicity. Swiss albino male mice were exposed to Orange G through intraperitoneal injections. Bone marrow cells isolated from femora were analyzed for sister chromatid exchanges (SCE) and chromosome aberrations. The results showed that the incidence of SCEs and chromosome aberrations were significantly higher than controls at certain concentrations. 25 mg/kg of Orange G was found to be the minimum effective dose for the induction of both SCEs and chromosome aberrations. Orange G is thus found to be clastogenic and genotoxic in vivo in mice.
Giri
A K
AK
Department of Botany, University of Calcutta, India.
Mukherjee
A
A
Talukder
G
G
Sharma
A
A
D000818:Animals; D001391:Azo Compounds / Q000633:toxicity*; D001853:Bone Marrow / Q000187:drug effects* / Q000648:ultrastructure; D002869:Chromosome Aberrations / Q000187:drug effects; D005505:Food Coloring Agents / Q000633:toxicity*; D008297:Male; D051379:Mice; D009153:Mutagens; D012854:Sister Chromatid Exchange / Q000187:drug effects; D013223:Statistics as Topic
D001391:Azo Compounds; D005505:Food Coloring Agents; D009153:Mutagens; C008710:Orange G