title: Mechanism of hematuria. II. A scanning electron microscopic demonstration of the passage of blood cells through a glomerular capillary wall in rabbit Masugi nephritis.
abstract: A model of hematuria was established in rabbits. An accelerated form of unilateral Masugi nephritis was induced in 10 New Zealand white rabbits by an intravenous injection of duck antirabbit kidney serum and by ligating the left renal artery immediately after the injection of the antibody. All 10 rabbits became hematuric 1-2 weeks after the injection of the antibody and red blood cell (RBC) casts were found in the urinary sediment of all these animals. An ultrastructural examination of renal glomeruli by transmission electron microscopy revealed the transcapillary passage of polymorphonuclear leukocytes through the gaps of the glomerular basement membrane (GBM). RBC were found in the urinary space in 50% of the glomeruli observed by scanning electron microscopy (SEM) and the passage of leukocytes and RBCs through the glomerular capillary wall was also observed. Gaps in the GBM became clearer after the removal of cellular components by detergents. In control rabbits, no RBCs could be observed in the urinary space, and isolated GBM were intact by SEM. These data further support the hypothesis that in rabbit Masugi nephritis hematuria is a result of the passage of RBCs through gaps in the GBM.
authors.affiliation: Third Department of Medicine, Okayama University Medical School, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Makino
authors.forename: S
authors.initials: S
authors.lastname: Nishimura
authors.forename: M
authors.initials: M
authors.lastname: Takaoka
authors.forename: Z
authors.initials: Z
authors.lastname: Ota
mesh_terms: D000818:Animals; D001485:Basement Membrane / Q000648:ultrastructure; D002196:Capillaries / Q000473:pathology / Q000648:ultrastructure*; D004195:Disease Models, Animal; D005921:Glomerulonephritis / Q000473:pathology*; D006417:Hematuria / Q000473:pathology*; D007678:Kidney Glomerulus / Q000098:blood supply* / Q000473:pathology / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D011817:Rabbits