title: NMR study of the interaction of retinoids with phospholipid bilayers.
abstract: The interaction of three vitamin A derivatives or retinoids: all-trans-retinoic acid, 13-cis-retinoic acid and retinol with multilamellar phospholipid bilayers was studied using a combination of 2H- and 31P-NMR measurements. The following model membrane systems were used: (1) dipalmitoylphosphatidylcholine (DPPC) bilayers; (2) bilayers composed of a mixture of DPPC and bovine heart phosphatidylcholine (PC); (3) mixed PC/phosphatidylethanolamine (PE) bilayers. Only a weak interaction was observed between 13-cis-retinoic acid and DPPC membranes. Addition of all-trans-retinoic acid at a molar ratio of 1:2 to the lipid causes a small decrease (5 C degrees) in the gel to liquid crystalline phase-transition temperature of DPPC, a small increase in the order parameters of the lipid side-chains of single component bilayers and no measurable effect in the other lipid systems studied. Considerably larger perturbation in the lipid bilayer structure is introduced by addition of retinol which, at a molar ratio of 1:2 to the lipid, lowered the gel to liquid crystalline phase-transition temperature of DPPC by 21 C degrees and caused a decrease of order parameters of the lipid side-chains in all three lipid bilayer systems. These effects are consistent with intercalation of retinol molecules into the bilayer interior. The results for the mixed PC/PE bilayers indicate that the presence of retinol caused lateral separation of PE- and retinol-enriched regions.
authors.affiliation: Department of Biology, University of California, Riverside 92521.
authors.forename: H
authors.initials: H
authors.lastname: De Boeck
authors.forename: R
authors.initials: R
authors.lastname: Zidovetzki
mesh_terms: D015060:1,2-Dipalmitoylphosphatidylcholine; D008051:Lipid Bilayers; D009682:Magnetic Resonance Spectroscopy; D008563:Membrane Lipids; D010714:Phosphatidylethanolamines; D010743:Phospholipids; D012176:Retinoids; D013696:Temperature
chemical_list: D008051:Lipid Bilayers; D008563:Membrane Lipids; D010714:Phosphatidylethanolamines; D010743:Phospholipids; D012176:Retinoids; D015060:1,2-Dipalmitoylphosphatidylcholine