Autoradiographic studies of the renal clearance of circulating type I collagen fragments in the rat.
Cyanogen bromide (CNBr) peptides of [3H]-labelled type I collagen isolated from young rat skin were injected into adult rats via the tail vein. After 1, 5 and 10 minutes, the fate of the circulating radioactive peptides was investigated by measurement of the tissue distribution of radioactivity. Rapid removal of peptides from the circulation and their concomitant accumulation in the kidney proximal tubule epithelia was confirmed by autoradiography of kidney sections at the light microscope level. Immunopositive material was also identified and localized in proximal tubules using antibodies directed against denatured type I collagen chains and their CNBr degradation products. Ultrastructural autoradiography indicated that rapid removal of collagen peptides from the glomerular filtrate occurs via the brush border, apical vacuoles, and lysosomes.
Rucklidge
G J
GJ
Rowett Research Institute, Bucksburn, Aberdeen, Scotland.
Riddoch
G I
GI
Williams
L M
LM
Robins
S P
SP
D000818:Animals; D001345:Autoradiography; D003094:Collagen / Q000493:pharmacokinetics*; D003488:Cyanogen Bromide; D007668:Kidney / Q000378:metabolism*; D007687:Kidney Tubules, Proximal / Q000378:metabolism / Q000648:ultrastructure; D010446:Peptide Fragments / Q000493:pharmacokinetics*; D051381:Rats; D011919:Rats, Inbred Strains; D012867:Skin; D014018:Tissue Distribution; D014316:Tritium
D010446:Peptide Fragments; D014316:Tritium; D003094:Collagen; D003488:Cyanogen Bromide