Cimetidine and mucosal cell renewal in the duodenum.
Cimetidine may protect gastric and duodenal mucosa by increasing cell turnover. Mucosal cell turnover in the duodenum of 7 control rats receiving intraperitoneal injections of normal saline for 6 days was compared with 8 rats receiving cimetidine for 6 days (500 mg/kg/24 h). Cell proliferation was assessed by autoradiography after injection of tritiated thymidine. The 'leading edge' was defined as the highest tritium-labelled nucleus in the crypt-villus (D/V) column expressed as a percentage of the complete C/V column. Ten C/V columns were measured in each rat. The number of tritium-labelled nuclei in 10 complete C/V columns was also counted in each rat. In the controls the median leading edge was 74.8% (25% quartile 70.6%, 75% quartile 76.2%) and in the rats receiving cimetidine 73.4% (25% quartile 72.1%, 75% quartile 75.9%). The median labelled cell counts were 2,052 (25% quartile 2,008, 75% quartile 2,173) and 1,999 (25% quartile 1,822, 75% quartile 2,041), respectively. These differences were not significant (p greater than 0.1; Mann-Whitney U test). Duodenal mucosal proliferation was assessed in 19 rats 48 h after two subcutaneous injections of 20 mg cysteamine and in another 9 after the same dose of cysteamine plus cimetidine (500 mg/kg/24 h). The median leading edge was 90.1% (25% quartile 84.4%, 75% quartile 92.8%) in cysteamine-treated rats and 76.7% (25% quartile 71%, 75% quartile 79.9%) in rats receiving cysteamine plus cimetidine.(ABSTRACT TRUNCATED AT 250 WORDS)
Smedley
F H
FH
Academic Surgical Unit, St. Stephen's Hospital, London, UK.
Wastell
C
C
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002927:Cimetidine / Q000494:pharmacology*; D003543:Cysteamine / Q000494:pharmacology; D004386:Duodenum / Q000166:cytology* / Q000473:pathology; D005260:Female; D007413:Intestinal Mucosa / Q000166:cytology* / Q000473:pathology; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values
D003543:Cysteamine; D002927:Cimetidine