The organization of the cardiac ganglion of the axolotl (Ambystoma mexicanum).
The heart of the axolotl Ambystoma mexicanum was studied with histochemical methods to determine the distribution of neurons containing acetylcholine esterase, catecholamines and 5-hydroxytryptamine. The cardiac ganglion is made up of cholinergic nerve fibers and somata, and of catecholaminergic fibers. Small intensely fluorescent cells were found along blood vessels in the pericardial wall at the base of the heart, but not in the heart itself, except, in a few instances, in the region bordering the pericardial wall. Both the cholinergic and the catecholaminergic innervation of the heart were poorly developed at hatching and reached their mature state after a few months. Cholinesterase staining fibers appeared several weeks before catecholaminergic fibers. The number of postganglionic cholinergic neurons in the heart increased several-fold during the first month after hatching. Histofluorescence studies of organ cultures suggested that all the catecholamine present in the heart are of extrinsic origin. Liquid chromatography with electrochemical detection demonstrated that the dominant catecholamine in the heart is norepinephrine. No neurons containing 5-hydroxytryptamine were found.
Melinek
R
R
Medical Sciences Program, Indiana University Bloomington 47405.
Mirolli
M
M
D000110:Acetylcholinesterase / Q000378:metabolism; D000557:Ambystoma / Q000033:anatomy & histology*; D000558:Ambystoma mexicanum / Q000033:anatomy & histology* / Q000254:growth & development; D000818:Animals; D002395:Catecholamines / Q000378:metabolism; D005724:Ganglia / Q000033:anatomy & histology* / Q000166:cytology; D006321:Heart / Q000033:anatomy & histology* / Q000254:growth & development; D006651:Histocytochemistry; D008856:Microscopy, Fluorescence; D008857:Microscopy, Interference; D009206:Myocardium / Q000378:metabolism; D009474:Neurons / Q000201:enzymology / Q000378:metabolism; D009994:Osmolar Concentration
D002395:Catecholamines; D000110:Acetylcholinesterase