title: Role of sodium ions in angiotensin tachyphylaxis in the guinea-pig ileum and taenia coli.
abstract: We have examined the responsiveness of the guinea-pig ileum and taenia coli to angiotensin (ANG) analogues that were previously shown to be either able ([1-sarcosine]-ANG, Sar1-ANG) or unable ([2-lysine]-ANG, Lys2-ANG) to induce tachyphylaxis in the ileum. The taenia coli, in which tachyphylaxis had not been previously shown to occur, was strongly tachyphylactic to Sar1-ANG, but not to Lys2-ANG. The contractile responses of the ileum, as well as the contractile and electrical (sucrose-gap) events in the taenia coli, in response to the two ANG analogues, were used to investigate the tachyphylactic phenomenon and the role of Na+ in the manifestation. Relaxation of the ileum and repolarizations of the taenia coli were faster after treatment with Lys2-ANG than after Sar1-ANG. In the tachyphylactic state, relaxation and repolarization after Sar1-ANG became as fast as after Lys2-ANG. In "low-Na+" (80 mmol/l) medium, as well as in ouabain-treated preparations, the responses of the ileum to ANG analogues were similar to those of tissues in the tachyphylactic state. Addition of Ca2+ to taenia coli preparations previously treated with the two ANG analogues in Ca2+-free medium, caused contractile and electrical responses only in the case of Sar1-ANG. It is proposed that ANG tachyphylaxis is due to changes at the level of the receptor causing increased Na+ permeability which leads to a decreased Na+ gradient across the cell membrane.
authors.affiliation: Department of Biophysics, Escola Paulista de Medicina, São Paulo, Brazil.
authors.forename: T B
authors.initials: TB
authors.lastname: Paiva
authors.forename: A C
authors.initials: AC
authors.lastname: Paiva
authors.forename: S I
authors.initials: SI
authors.lastname: Shimuta
mesh_terms: D000803:Angiotensin I / Q000031:analogs & derivatives / Q000494:pharmacology; D000804:Angiotensin II / Q000031:analogs & derivatives / Q000494:pharmacology / Q000502:physiology*; D000818:Animals; D003106:Colon / Q000187:drug effects; D004558:Electric Stimulation; D005260:Female; D006168:Guinea Pigs; D007082:Ileum / Q000187:drug effects; D066298:In Vitro Techniques; D007537:Isometric Contraction / Q000187:drug effects; D008297:Male; D009126:Muscle Relaxation / Q000187:drug effects; D009130:Muscle, Smooth / Q000502:physiology*; D012964:Sodium / Q000502:physiology*; D013618:Tachyphylaxis
chemical_list: D000804:Angiotensin II; C058393:angiotensin II, Lys(2)-; C051901:angiotensin I, Sar(1)-; D000803:Angiotensin I; D012964:Sodium