Localization of antibody-secreting cells against human colon adenocarcinoma cell lines using the enzyme-linked immunospot assay.
The enzyme-linked immunospot assay was used to detect antibody-secreting cells from mice immunized against three human colon adenocarcinoma cell lines (Colo-205, SW 620, and SW 1116) on dishes coated with these cell lines. Antibody-secreting cells were detected by the development of a dark granular precipitate ('spot') using immunoenzymatic techniques where secreted antibody had bound to cell surface antigen. Spots can be detected by the naked eye and can be enumerated under low-power magnification. This method is specific, easily performed, versatile, and, together with conventional enzyme-linked immunosorbent assay techniques, allows for quantification of secreted antibody.
Larson
L N
LN
Department of Medical Microbiology, University of Göteborg, Sweden.
Johansson
C
C
Lindholm
L
L
D000230:Adenocarcinoma / Q000032:analysis* / Q000276:immunology / Q000473:pathology; D000818:Animals; D000921:Antibody-Producing Cells / Q000378:metabolism*; D002460:Cell Line; D003110:Colonic Neoplasms / Q000032:analysis* / Q000276:immunology / Q000473:pathology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007958:Leukocyte Count; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009368:Neoplasm Transplantation; D015203:Reproducibility of Results; D013154:Spleen