The endogenous muscarinic acetylcholine receptor in Xenopus oocytes is of the M3 subtype.
The muscarinic acetylcholine receptor of the Xenopus oocyte was characterized electrophysiologically. Iontophoretic responses to acetylcholine were inhibited by the muscarinic antagonists 4-DAMP, pirenzepine and AF-DX 116 at respective IC50 values of 7 nM, 15 microM and 2 microM. This antagonist sensitivity order indicates that the receptor is of the M3 muscarinic subtype.
Van Wezenbeek
L A
LA
CNS Pharmacology R&D Laboratory, Organon Int. B.V., Oss, The Netherlands.
Tonnaer
J A
JA
Ruigt
G S
GS
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D005260:Female; D066298:In Vitro Techniques; D008564:Membrane Potentials / Q000187:drug effects; D009865:Oocytes / Q000378:metabolism*; D010880:Piperidines / Q000494:pharmacology; D010890:Pirenzepine / Q000031:analogs & derivatives / Q000494:pharmacology; D011950:Receptors, Cholinergic / Q000378:metabolism*; D014982:Xenopus laevis
D010880:Piperidines; D011950:Receptors, Cholinergic; D010890:Pirenzepine; C042375:4-diphenylacetoxy-1,1-dimethylpiperidinium; D000109:Acetylcholine; C048593:otenzepad