Secretion of newly synthesized proteins into the extracellular fluid of the rabbit hippocampus.
Extracellular proteins were collected continuously from the hippocampus of the rabbit by slow perfusion of a protein-permeable thin tubing. After pulse labelling with radioactive amino acids via the tubing a rapid appearance of labelled proteins was seen in the extracellular fluid. The half-life of the proteins in the extracellular space varied between 15 min and 2 h. Protein fractions were separated with gel electrophoresis or HPLC. The extracellular fraction differed distinctly from intracellular proteins with respect to the labelling pattern.
Nyström
B
B
Institute of Neurobiology, University of Göteborg, Sweden.
Karlsson
J O
JO
Hamberger
A
A
D000818:Animals; D005110:Extracellular Space / Q000378:metabolism*; D006624:Hippocampus / Q000378:metabolism*; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000378:metabolism*; D011817:Rabbits; D013997:Time Factors
D009419:Nerve Tissue Proteins