A new monoclonal antibody, UFT-4, reacting with rat Kupffer cells. Immunohistochemical and immunoelectron microscopical analysis with reestimation of the reticuloendothelial system.
A new monoclonal antibody, designated UFT-4, reacting with rat Kupffer cells, was produced using sinusoidal liver-cell fraction as an immunogen. Immunohistochemically, UFT-4 reacted with Kupffer cells, interdigitating cells (IDCs), sinus endothelial cells of the spleen, a proportion of reticulum cells, smooth muscle fibers, choroid plexus epithelia and some macrophages. Immunoelectron microscopy disclosed two types of intracytoplasmic positivity: a striated type and a diffuse type. Both appeared to depend on variations in the quantity and distribution of the same antigen. On the other hand, UFT-4 gave a negative result for blood monocytes and most macrophages in lymphatic sinuses, lymphoid follicles, splenic red pulp and loose connective tissue. Cells positive for UFT-4 were mostly considered to belong to a narrow spectrum of the classical reticuloendothelial system, explaining the close relationship existing between some endothelia and some macrophages or reticulum cells. SDS-PAGE analysis of immunoprecipitates showed that the antigen reacting with UFT-4 was of molecule of 36-42 kDa under reducing conditions in the presence of 2-mercaptoethanol. The present results suggest that UFT-4 will be very useful for the study of Kupffer cells and for reconsidering their origin and destination.
Ukai
K
K
Department of Pathology, Yamagata University School of Medicine, Japan.
Terashima
K
K
Fujii
Y
Y
Imai
Y
Y
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis*; D000941:Antigens / Q000032:analysis; D007150:Immunohistochemistry; D007728:Kupffer Cells / Q000276:immunology* / Q000502:physiology; D008099:Liver / Q000276:immunology / Q000378:metabolism; D008198:Lymph Nodes / Q000276:immunology / Q000378:metabolism; D008264:Macrophages / Q000276:immunology / Q000378:metabolism; D008297:Male; D008854:Microscopy, Electron / Q000379:methods; D012157:Mononuclear Phagocyte System / Q000502:physiology; D009852:Omentum / Q000276:immunology / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013154:Spleen / Q000276:immunology / Q000378:metabolism; D013950:Thymus Gland / Q000276:immunology / Q000378:metabolism
D000911:Antibodies, Monoclonal; D000941:Antigens