title: Effects of pantothenic acid on fibroblastic cell cultures.
abstract: To evaluate the effects of pantothenic acid during wound healing processes, fibroblastic cell cultures originating from foreskin were established and subcultured by trypsinization. PA (40 micrograms/ml) was added to the basal culture medium. The cell proliferation was estimated by cell count and determination of 3H-thymidine incorporation. The protein synthesis and secretion were determined by dosage in the cells and in the culture medium. When PA was added to the medium, a significant increase of cell proliferation and of 3H-thymidine incorporation was observed mainly during the first few days. PA also stimulated intracellular protein synthesis, but did not induce a release of proteins in the culture medium. The exact mechanism involved in this phenomenon remains unclear at this time.
authors.affiliation: Inserm Unité 61, Hôpital Civil, Strasbourg, France.
authors.forename: B
authors.initials: B
authors.lastname: Lacroix
authors.forename: E
authors.initials: E
authors.lastname: Didier
authors.forename: J F
authors.initials: JF
authors.lastname: Grenier
mesh_terms: D002455:Cell Division / Q000187:drug effects*; D002478:Cells, Cultured; D004261:DNA Replication / Q000187:drug effects*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D006801:Humans; D007700:Kinetics; D010205:Pantothenic Acid / Q000494:pharmacology*; D012867:Skin / Q000166:cytology; D013936:Thymidine / Q000378:metabolism
chemical_list: D010205:Pantothenic Acid; D013936:Thymidine