Predicting the fertilising ability of avian semen: comparison of a simple colourimetric test with other methods for predicting the fertilising ability of fowl semen.
1. The rate of tetrazolium dye-reduction by fowl spermatozoa measured by an objective colourimetric assay was shown to correlate strongly with sperm motility, morphology, ATP content and fertilising ability. 2. Although dye-reduction appeared less well correlated with fertilising ability than the other variables, the method for its determination has many practical advantages for the assessment of semen quality in poultry.
Chaudhuri
D
D
AFRC Institute of Animal Physiology and Genetics Research, Roslin, Midlothian, Scotland.
Wishart
G J
GJ
Lake
P E
PE
Ravie
O
O
D000255:Adenosine Triphosphate / Q000032:analysis*; D000818:Animals; D002645:Chickens / Q000502:physiology*; D003124:Colorimetry / Q000379:methods; D005306:Fertilization; D008297:Male; D012661:Semen / Q000032:analysis*; D013081:Sperm Motility; D013094:Spermatozoa / Q000378:metabolism*; D013778:Tetrazolium Salts / Q000378:metabolism
D013778:Tetrazolium Salts; C013574:iodonitrotetrazolium; D000255:Adenosine Triphosphate