title: UV-induced growth inhibition in the trypanosomatid Crithidia fasciculata.
abstract: The effects of short ultraviolet (253 nm) radiation on the growth of the trypanosomatid flagellate Crithidia fasciculata were studied in liquid medium and nutrient agar plates. Cell duplication was completely inhibited after exposure of the flagellates to doses equal to or higher than 50 J/m2. The UV-induced lag period was dose-dependent. Survival was reduced to 1% after exposure of the parasites to 200 J/m2. Ultrastructural changes after the lag period were studied by transmission electron microscopy. Changes of the kinetoplast network structure and sometimes of the mitochondrial matrix were observed. The existence of DNA repair mechanisms in this protozoan is discussed.
authors.affiliation: Departamento de Biologia Geral, Universidade Federal de Pernambuco, Recife, PE, Brasil.
authors.forename: A M
authors.initials: AM
authors.lastname: Barros
authors.forename: P P
authors.initials: PP
authors.lastname: Andrade
mesh_terms: D000818:Animals; D002455:Cell Division / Q000528:radiation effects; D003421:Crithidia / Q000254:growth & development / Q000528:radiation effects* / Q000648:ultrastructure; D003470:Culture Media; D004260:DNA Repair / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D008854:Microscopy, Electron; D011829:Radiation Dosage; D012044:Regression Analysis; D014466:Ultraviolet Rays
chemical_list: D003470:Culture Media