Blood cell superoxide dismutase and enolase activities as markers of alcoholic and nonalcoholic liver diseases.
Monitoring of chronic alcoholism would be facilitated by using sensitive biochemical markers in blood cells, mainly to detect differences between alcoholic subjects with or without liver injury. We propose two types of markers: the first one is superoxide dismutase (SOD) activity involved in the conversion of superoxide radicals (O2-.) formed during acetaldehyde oxidation by xanthine oxidase after chronic alcohol consumption; the second one is enolase activity with both isoenzyme forms: nonneuronal enolase (NNE) and neuron specific enolase (NSE) which has been shown to be modified in many injuries related to the glycolytic pathways. For SOD activity we found a significant increase in alcoholic patients with liver injury and mainly in cirrhotic patients with ascitis. Both enolase activities were also found to be significantly increased in alcoholic patients with liver injury but NNE activity was also increased in alcoholics without apparent liver disease. Our results suggest that increased activity of SOD and NSE in blood cells may be related to liver injury mainly in alcoholism while increased NNE activity may also be a marker of alcohol abuse without liver injury.
Ledig
M
M
Centre de Neurochimie du CNRS, Strasbourg, France.
Doffoel
M
M
Doffoel
S
S
Kopp
P
P
Bockel
R
R
Mandel
P
P
D000328:Adult; D000368:Aged; D000437:Alcoholism / Q000201:enzymology; D015415:Biomarkers / Q000378:metabolism; D001773:Blood Cells / Q000201:enzymology*; D006801:Humans; D008107:Liver Diseases / Q000201:enzymology*; D008108:Liver Diseases, Alcoholic / Q000201:enzymology*; D008875:Middle Aged; D010751:Phosphopyruvate Hydratase / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism*
D015415:Biomarkers; D013482:Superoxide Dismutase; D010751:Phosphopyruvate Hydratase