title: Structure of P401 (mast cell degranulating peptide) in solution.
abstract: P401 (also known as mast cell degranulating protein, MCD) is a minor component of honeybee venom. Its primary structure is related to that of apamin. We have studied the structure of P401 in solution by high-resolution two-dimensional 1H-NMR spectroscopy. Almost all the backbone proton resonances have been assigned by sequential assignment strategy. Analysis of NOEs shows that P401 has a conformation very similar to that of apamin. N-terminal residues Ile-1-Cys-5 are in an extended conformation and residues His-13-Asn-22 on the C-terminus are in an alpha-helical structure. These two secondary structural elements are connected by two tight turns.
authors.affiliation: Department of Chemistry, University of California, Berkeley 94720.
authors.forename: N V
authors.initials: NV
authors.lastname: Kumar
authors.forename: D E
authors.initials: DE
authors.lastname: Wemmer
authors.forename: N R
authors.initials: NR
authors.lastname: Kallenbach
mesh_terms: D000595:Amino Acid Sequence; D001514:Bee Venoms; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D009498:Neurotoxins; D010455:Peptides; D011487:Protein Conformation; D012996:Solutions
chemical_list: D001514:Bee Venoms; D009498:Neurotoxins; D010455:Peptides; D012996:Solutions; C008331:mast cell degranulating peptide