Partitioning behaviour in aqueous two-phase systems and fractionation by counter-current distribution of chick-embryo erythrocytes with numerical resolution of distribution curves.
The partition of chick-embryo and young-chick erythrocytes in dextran-poly-(ethylene glycol) two-phase systems depends on the interfacial tension and electrical potential differences between the phases. Counter-current distribution with charged 5% dextran-poly(ethylene glycol) systems has proved to be an adequate method for the separation of primitive and definitive erythrocytes present in chick embryos when a phase settling time of 20 min is used. The computer-aided numerical resolution of experimental curves has shown the existence of subpopulations which could not have been detected by using conventional methods.
Ferrer
E
E
Departamento de Bioquímica, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain.
Martin
M
M
Alonso
R
R
Luque
J
J
D000818:Animals; D002642:Chick Embryo; D002645:Chickens; D003377:Countercurrent Distribution; D004910:Erythrocyte Membrane; D004912:Erythrocytes / Q000032:analysis*; D010710:Phosphates
D010710:Phosphates