Thyroid hormone metabolism in neuron-enriched primary cultures of fetal rat brain cells.
The metabolism of thyroxine (T4) by cultures of embryonic-rat brain cells grown in a chemically defined medium was studied. Cells in these cultures were predominantly neurons, characterized by the developmental increase of the binding of [3H]flunitrazepam to the high-affinity (0.67 nM) benzodiazepine neuronal receptors. The cultures also contained astrocytes, characterized by immunological studies using an anti-glial fibrillary acidic protein (GFAp) and by the increase in glutamine synthetase (GS). Incubation of the cells, in situ, with 125I-labelled 3,5,3'-triiodothyronine (T3) showed the presence of a single class of high-affinity nuclear receptors for T3 with a maximal binding capacity of 270-470 fmol T3/mg DNA and a Kd of 63 +/- 13 pM. Cells incubated in situ with 50 pM [125I]T4 actively metabolized the hormone. The major metabolite, 3,3',5'-triiodothyronine (rT3) (159 +/- 43 fmol/4 h/mg DNA), was almost completely released into the medium. T3 was a minor metabolite (77 +/- 3 fmol/4 h/mg DNA), 75% of which accumulated in the cells. Of this T3, 35% was bound to the nuclear receptors after 4 h of incubation. In vitro assays showed that the 5'-deiodinase activity increased during culture and the 5-deiodinase decreased slightly. Cytosine-arabinoside (ARAc) treatment of the cultures reduced the DNA content per culture dish, corresponding to a fall in the number of GFAp-positive cells (astrocytes) and to a decrease in GS. A small increase in the number of benzodiazepine sites was observed. ARAc treatment markedly reduced the T3 production (14.5 +/- 0.7 fmol/4 h/mg DNA) and did not change the rT3 production. We suggest that T4 is metabolized to T3 in astrocytes, taken up by neurons and binds to their nuclear receptors.
Courtin
F
F
Institut National de la Santé et de la Recherche Médicale, Centre Hospitalier du Kremlin-Bicêtre, France.
Chantoux
F
F
Francon
J
J
D000818:Animals; D001253:Astrocytes / Q000201:enzymology / Q000378:metabolism / Q000648:ultrastructure; D001921:Brain / Q000166:cytology / Q000196:embryology*; D002478:Cells, Cultured; D003561:Cytarabine / Q000494:pharmacology; D005333:Fetus / Q000378:metabolism; D007453:Iodide Peroxidase / Q000378:metabolism; D009474:Neurons / Q000201:enzymology / Q000378:metabolism* / Q000648:ultrastructure; D051381:Rats; D011919:Rats, Inbred Strains; D013974:Thyroxine / Q000378:metabolism*; D014284:Triiodothyronine / Q000378:metabolism*
D003561:Cytarabine; D014284:Triiodothyronine; D007453:Iodide Peroxidase; D013974:Thyroxine