title: Bovine mitochondrial tRNAPhe, tRNASer (AGY) and tRNASer (UCN): preparation using a new detection method and their properties in aminoacylation.
abstract: Bovine mitochondrial tRNAPhe, tRNASer (AGY), and tRNASer (UCN) possessing unusual structures were purified using a new hybridization assay system and their properties in aminoacylation were examined. Bovine mitochondrial phenyl-alanyl- and seryl-tRNA synthetases could aminoacylate the same amino acid-specific tRNAs obtained not only from the mitochondria but also from other sources such as E. coli, Thermus thermophilus, bovine and yeast cytosols and archaebacteria, Sulfolobus acidocaldarius. On the contrary, none of both bacterial and cytosolic synthetases could aminoacylate the same amino acid specific tRNAs from the heterologous sources with some exceptions. We consider that the bovine mitochondrial aminoacyl-tRNA synthetases have considerably simple recognition mechanism toward the substrate tRNAs compared with the non-mitochondrial ones. This mechanism may be correlated with the occurrence of structural varieties of the mitochondrial tRNA species with unusual structures.
authors.affiliation: Department of Industrial Chemistry, Faculty of Engineering, University of Tokyo, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Kumazawa
authors.forename: T
authors.initials: T
authors.lastname: Yokogawa
authors.forename: K
authors.initials: K
authors.lastname: Miura
authors.forename: K
authors.initials: K
authors.lastname: Watanabe
mesh_terms: D000215:Acylation; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D008930:Mitochondria, Liver / Q000378:metabolism*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D012345:RNA, Transfer, Amino Acid-Specific / Q000235:genetics*; D012360:RNA, Transfer, Phe / Q000235:genetics* / Q000302:isolation & purification; D012362:RNA, Transfer, Ser / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D012345:RNA, Transfer, Amino Acid-Specific; D012360:RNA, Transfer, Phe; D012362:RNA, Transfer, Ser