Effect of ouabain on norepinephrine-induced contractions of isolated rabbit aorta.
The acute potentiating effect of ouabain on norepinephrine (NE) induced contractions of isolated rabbit aorta was investigated. Ouabain, at concentrations of 3 X 10(-7) to 10(-5) mol/l potentiated the vasoconstrictor effect of NE as demonstrated by a shift to the left of the NE concentration-response curve in a parallel manner and a concentration-dependent increase in the EC50 ratio. A significant increase in maximal contractility was observed by ouabain at 3 X 10(-6) and 10(-5) mol/l. Removal of the endothelium did not alter the effect of 10(-5) mol/l ouabain. When the NE concentration-response curves were again determined after ouabain had been washed out, it was found that the ouabain-induced increase in EC50 ratios returned to control values, while the increase in maximal contractility did not. The slopes of the NE concentration-response curves were not significantly different in the presence of ouabain than control values, but were higher when obtained after ouabain had been washed out.
Oktay
S
S
Department of Pharmacology, School of Medicine, Ankara University, Turkey.
Ilhan
M
M
D000818:Animals; D001013:Aorta, Thoracic / Q000187:drug effects; D005260:Female; D066298:In Vitro Techniques; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D009131:Muscle, Smooth, Vascular / Q000187:drug effects*; D009638:Norepinephrine / Q000494:pharmacology*; D010042:Ouabain / Q000494:pharmacology*; D011817:Rabbits
D010042:Ouabain; D009638:Norepinephrine