Inhomogeneous translational diffusion of monoclonal antibodies on phospholipid Langmuir-Blodgett films.
The translational mobility of fluorescent-labeled monoclonal antibodies specifically bound to supported phospholipid bilayers containing hapten-conjugated phospholipids has been measured as a function of the surface concentration of bound antibodies using fluorescence recovery after photobleaching. Fluorescence recovery curves are fit well by a model that assumes the presence of two populations of antibodies with different lateral diffusion coefficients. The larger diffusion coefficient equals 3.5 x 10(-9) cm2/s, the smaller diffusion coefficient ranges from 1.5 x 10(-9) cm2/s to 2.5 x 10(-10) cm2/s, and the fractional fluorescence recovery associated with the smaller coefficient increases from approximately 0 to approximately 0.7 with increasing concentration of bound antibody. These results suggest that complexes of haptenated phospholipids and antibodies in phospholipid Langmuir-Blodgett films form clusters or domains in a concentration-dependent fashion.
Wright
L L
LL
Department of Physics, University of North Carolina, Chapel Hill 27599.
Palmer
A G
AG
Thompson
N L
NL
D000911:Antibodies, Monoclonal; D004058:Diffusion; D006241:Haptens; D007700:Kinetics; D008051:Lipid Bilayers; D010743:Phospholipids; D011485:Protein Binding; D013050:Spectrometry, Fluorescence
D000911:Antibodies, Monoclonal; D006241:Haptens; D008051:Lipid Bilayers; D010743:Phospholipids