title: Scope and limitations of rotating-frame nuclear Overhauser enhancement spectroscopy applied to oligosaccharides.
abstract: Two-dimensional rotating-frame nuclear Overhauser enhancement spectroscopy has been applied to milk oligosaccharides containing the blood-group determinants Lea, Led, or Leb. The results indicate a large number of dipolar interactions that are consistent with the preferred conformations described for these blood-group determinants. Despite careful experimental conditions, coherent transfer processes are not suppressed completely, allowing only a qualitative evaluation of the nuclear Overhauser data. The 1H- and 13C-n.m.r. spectra are assigned by comparison with literature data and by application of two-dimensional 13C-1H shift-correlation spectroscopy.
authors.affiliation: Department of Bio-Organic Chemistry, University of Utrecht, The Netherlands.
authors.forename: J
authors.initials: J
authors.lastname: Breg
authors.forename: D
authors.initials: D
authors.lastname: Romijn
authors.forename: J F
authors.initials: JF
authors.lastname: Vliegenthart
authors.forename: G
authors.initials: G
authors.lastname: Strecker
authors.forename: J
authors.initials: J
authors.lastname: Montreuil
mesh_terms: D009682:Magnetic Resonance Spectroscopy; D009844:Oligosaccharides / Q000032:analysis*; D013057:Spectrum Analysis
chemical_list: D009844:Oligosaccharides