Two-dimensional immunoblotting analysis and immunocytochemical localization of the secretory polypeptide 7B2 in adrenal medulla.
The soluble proteins of bovine chromaffin granules were subjected to 2D-electrophoresis followed by immunoblotting with an antiserum against the pituitary peptide 7B2. One immunoreactive spot was visualized at a position corresponding to a molecular weight of 24,000 and to a pI of 5.2. Using peroxidase-antiperoxidase (PAP) pre-embedding immunocytochemical technique for electron microscopy, 7B2 has been localized within secretory granules with diameters of approximately 115 and 190 nm in noradrenergic and adrenergic cells respectively. These data establish that in chromaffin granules 7B2 represents a minor component of the acidic proteins which include the chromogranins A and B, secretogranin II and the enkephalin-containing peptides.
Marcinkiewicz
M
M
J.A. De Sève Laboratories of Molecular and Biochemical Neuroendocrinology, Clinical Research Institute of Montreal, Que., Canada.
Fischer-Colbrie
R
R
Falgueyret
J P
JP
Benjannet
S
S
Seidah
N G
NG
Lazure
C
C
Winkler
H
H
Chrétien
M
M
D000313:Adrenal Medulla / Q000032:analysis*; D000818:Animals; D002417:Cattle; D002837:Chromaffin Granules / Q000032:analysis* / Q000648:ultrastructure; D002838:Chromaffin System / Q000032:analysis*; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D009419:Nerve Tissue Proteins; D051859:Neuroendocrine Secretory Protein 7B2; D010907:Pituitary Hormones / Q000032:analysis*
D009419:Nerve Tissue Proteins; D051859:Neuroendocrine Secretory Protein 7B2; D010907:Pituitary Hormones