Phosphorimeters for analysis of decay profiles and real time monitoring of exponential decay and oxygen concentrations.
A phosphorimeter which can be assembled at low cost from mainly commercially available components and which has better time resolution, data acquisition rate, sensitivity, and flexibility than commercially available instruments is described. As a phosphorescence analyzer the instrument can measure phosphorescence lifetimes ranging from approximately 30 microseconds to seconds from samples with variable intensity, excitation, and emission spectra and which may follow complex decay behavior. Configured as a phosphorescence monitor it is designed for fast, repetitive calculation of phosphorescence lifetime, assuming single-exponential decay, and can be used to calculate oxygen concentration in biological samples in real time.
Green
T J
TJ
Department of Biochemistry and Biophysics, School of Medicine, University of Pennsylvania, Philadelphia 19104.
Wilson
D F
DF
Vanderkooi
J M
JM
DeFeo
S P
SP
D008163:Luminescent Measurements; D055095:Optics and Photonics / Q000295:instrumentation*; D010100:Oxygen / Q000032:analysis*; D013997:Time Factors
D010100:Oxygen