Degradation of extracellular thymidine by cultured hepatocytes from rainbow trout (Salmo gairdneri).
1. Trout hepatocytes cultured as attached monolayers had low rates of [3H]-thymidine ([3H]-TdR) incorporation during replicative or repair synthesis of DNA. 2. Within 2 hr, most [3H]-TdR was metabolized by trout hepatocytes to a major product that eluted in advance of intact [3H]-TdR on Sephacryl S-200 columns. 3. Metabolism of [3H]-TdR by trout hepatocytes rapidly destroyed its ability to label replicating indicator cultures of proliferating rat hepatocytes. 4. These studies demonstrate that [3H]-TdR tracer assays for DNA synthesis cannot be reliably used in cultured trout hepatocytes which catabolize thymidine much more rapidly than do rat hepatocytes.
Kocal
T E
TE
Department of Pathology, University of Guelph, Ontario, Canada.
Crane
T L
TL
Quinn
B A
BA
Ferguson
H W
HW
Hayes
M A
MA
D000818:Animals; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D004260:DNA Repair; D004261:DNA Replication; D008099:Liver / Q000378:metabolism*; D051381:Rats; D012487:Salmonidae / Q000378:metabolism*; D013936:Thymidine / Q000378:metabolism*; D014337:Trout / Q000378:metabolism*
D004247:DNA; D013936:Thymidine