Hog cholera virus: identification and characterization of the viral RNA and the virus-specific RNA synthesized in infected swine kidney cells.
Virus-specific RNA in swine kidney cells infected with hog cholera virus and the RNA of gradient-purified virions were identified and characterized. The time curve for virus replication in SK-6 cells was established. The logarithmic phase of virus growth was between 4 and 12 h after virus adsorption. Virus production was maximum at 20 h after virus adsorption. Analysis of cytoplasmic RNA in a neutral agarose gel showed that the time curve for synthesis of a high molecular weight RNA in infected cells closely paralleled that for virus growth. Electrophoresis in an agarose-formaldehyde gel of [3H]uridine-labeled RNA, synthesized with actinomycin-D, showed that this high molecular weight RNA species was approximately 12 kilobases (kb) in length. RNA, extracted from virions purified in a glycerol-tartrate gradient, co-migrated in a neutral agarose gel with the 12 kb RNA species; moreover, it hybridized specifically with a cDNA clone prepared against the intracellular 12 kb RNA. These results confirm the virus-specific nature of the 12 kb RNA. Since no prominent subgenomic virus-specific RNA was identified in infected cells, an RNA species of this size may also act as a messenger RNA.
Moormann
R J
RJ
Central Veterinary Institute, Virology Department, Lelystad, The Netherlands.
Hulst
M M
MM
D000818:Animals; D002478:Cells, Cultured; D006692:Classical Swine Fever Virus / Q000235:genetics* / Q000302:isolation & purification; D003001:Cloning, Molecular; D004587:Electrophoresis, Agar Gel; D007668:Kidney / Q000166:cytology / Q000382:microbiology; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000096:biosynthesis / Q000302:isolation & purification; D013552:Swine; D013997:Time Factors; D014771:Virion / Q000235:genetics
D012367:RNA, Viral