Portable in vitro bath for an unsubmerged complete trachea.
An in vitro organ bath into which to insert a complete isolated trachea after surgical removal has been developed. The bath is portable, a very useful feature, to transport the trachea collected in the slaughterhouse to the laboratory. Moreover, the luminal surface of the mucosa is not submerged, but is air-filled, thus obtaining the physiological conditions closer to the one of the trachea in vivo. Thus, pharmacological investigations of drugs acting on physicochemical characteristic of mucus and on mucociliary clearance can be performed under controlled conditions.
Braga
P C
PC
Department of Pharmacology, School of Medicine, University of Milan, Italy.
Allegra
L
L
D000388:Air; D000818:Animals; D009093:Mucus / Q000378:metabolism; D009924:Organ Culture Techniques / Q000295:instrumentation*; D014132:Trachea / Q000187:drug effects / Q000502:physiology*