Cell proliferation and 3H-proline incorporation in periodontal ligament exposed to mechanical stress.
In order to study the metabolic processes induced in the periodontal ligament by mechanical influences, a tension spring was implanted in rats between the incisor and the first maxillary molar on the right-hand side, while the left maxilla of these animals as well as non-operated rats served as controls. Under such mechanical stress, there occurred at 3, 10 and 21 days after implantation a significant increase in the 3H-thymidine labeling index, which was demonstrable histoautoradiographically. A change in cell density was not discovered. Therefore, the increase in S-phase fraction as equally recorded in both pressure and tension zones is regarded as an expression of an enhanced cell turnover. Cell renewal in the periodontal ligament can be modified by inflammatory processes within the gingival region. There is a slight enlargement of the periodontal space in the tension zone. Under our experimental conditions, no change occurs in the silver grain number per cell after 3H-proline administration. The results indicate that, following the impact of orthodontic forces, the reactivity of periodontal cell proliferation as compared to collagen synthesis is enhanced.
Kunz
J
J
Institute of Pathological Anatomy, Charité Hospital, Humboldt University, Berlin, DDR.
Plascke
C
C
Duncker
M
M
D000818:Animals; D002455:Cell Division; D004261:DNA Replication; D010513:Periodontal Ligament / Q000166:cytology* / Q000378:metabolism; D011392:Proline / Q000378:metabolism; D014176:Protein Biosynthesis; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values; D013314:Stress, Mechanical; D013941:Thymine / Q000378:metabolism
D011392:Proline; D013941:Thymine