[Preparation of lysozyme immobilized on textile carriers and its use in treating suppurative wounds].
Immobilized forms of lysozyme were prepared by its covalent binding on dialdehyde cellulose and polycaproamide fibres as woven and knitted fabrics respectively. The preparations were estimated by the content of protein and bacteriolytic activity. The lysozyme activity per 1 g of the carrier and the protein content on dialdehyde cellulose were several times higher than those on polycaproamide while the specific activity of lysozyme on the polycaproamide carrier was somewhat higher than that on dialdehyde cellulose. The effect of the immobilized lysozyme in treatment of purulent wounds was studied on albino rats. It was shown that the periods of the wound healing with the use of the immobilized lysozyme were shorter than those with the use of native lysozyme. Cytological and morphological investigation of the wound wall confirmed the higher efficacy of the lysozyme immobilized forms in treatment of purulent wounds as compared to the use of the native enzyme.
Vlasov
L G
LG
Tolstykh
P I
PI
Ignatiuk
T E
TE
Razzakov
O N
ON
D000818:Animals; D001458:Bandages; D002209:Caprolactam / Q000031:analogs & derivatives; D002482:Cellulose / Q000031:analogs & derivatives; D004337:Drug Carriers; D004353:Drug Evaluation, Preclinical; D004800:Enzymes, Immobilized / Q000008:administration & dosage*; D008297:Male; D009113:Muramidase / Q000008:administration & dosage*; D011108:Polymers; D051381:Rats; D013203:Staphylococcal Infections / Q000188:drug therapy*; D013784:Textiles; D014946:Wound Infection / Q000188:drug therapy*
D004337:Drug Carriers; D004800:Enzymes, Immobilized; D011108:Polymers; C009916:nylon 6; D002209:Caprolactam; D002482:Cellulose; C058684:2,3-dialdehydocellulose; D009113:Muramidase
Poluchenie immobilizovannoogo na tekstil'nykh nositeliakh lizotsima i ego primenenie dlia lecheniia gnoĭnykh ran.