title: Biochemical characterization of lens crystallins from three mammalian species.
abstract: Lens crystallins were isolated from the homogenates of mammalian eye lenses derived from three different species by gel permeation chromatography and characterized by SDS-gel electrophoresis, isoelectric focusing, amino acid analysis and N-terminal sequence analysis. Five fractions corresponding to HM alpha-, alpha-, beta H-, beta L- and gamma-crystallins were obtained for the crystallins from these phylogenetically distant species. The native molecular masses for these purified fractions and their polypeptide compositions were determined by gel filtration and SDS-gel electrophoresis respectively, revealing the typical subunit compositions for each classified crystallin. The gel pattern of gamma-crystallins from the marmot lens appeared to be more complex than those of gibbon and deer lenses. Comparison of the amino acid contents of each orthologous class of mammalian crystallins with those of evolutionarily distant species still exhibited similarity in their amino acid compositions. The charge heterogeneity of each crystallin fraction can be detected by isoelectric focusing under denaturing conditions. N-terminal sequence analysis of the crystallin fractions revealed that all fractions except that of gamma-crystallin are N-terminally blocked. Extensive sequence similarity between mammalian gamma-crystallin polypeptides were found, which suggested the close relatedness of gamma-crystallins amongst different species of mammals and also established the heterogeneous nature of this multigene family.
authors.affiliation: Institute of Biochemical Sciences, National Taiwan University, Taipei, China.
authors.forename: S H
authors.initials: SH
authors.lastname: Chiou
authors.forename: W P
authors.initials: WP
authors.lastname: Chang
authors.forename: L M
authors.initials: LM
authors.lastname: Ting
authors.forename: T A
authors.initials: TA
authors.lastname: Lai
authors.forename: H K
authors.initials: HK
authors.lastname: Lin
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002850:Chromatography, Gel; D003459:Crystallins / Q000032:analysis*; D003670:Deer; D004591:Electrophoresis, Polyacrylamide Gel; D006922:Hylobates; D007525:Isoelectric Focusing; D008392:Marmota; D008969:Molecular Sequence Data
chemical_list: D003459:Crystallins