title: Hybridization of DNA probes to A. marginale isolates from different sources and detection in Dermacentor andersoni ticks.
abstract: DNA from the Washington, South-Idaho, Virginia and Florida isolates of Anaplasma marginale was hybridized to probes specific for Anaplasma centrale and A. marginale. The A. centrale probes AC-2 and AC-4 hybridized to identical bands on all of these isolates. The hybridization patterns suggests that the Virginia, Florida and the South African isolates are similar. A number of bands were obtained with the Washington isolate which differed from those obtained with the other isolates. Probe AC-2 could be developed to identify relatedness among Anaplasma isolates. Probe AC-2 detected A. marginale DNA in midgut material from infected Dermacentor andersoni ticks. No hybridization was obtained with DNA from salivary gland tissues from these infected ticks.
authors.affiliation: Molecular Biology Section, Veterinary Research Institute, Onderstepoort.
authors.forename: R E
authors.initials: RE
authors.lastname: Ambrosio
authors.forename: E S
authors.initials: ES
authors.lastname: Visser
authors.forename: Y
authors.initials: Y
authors.lastname: Koekhoven
authors.forename: K M
authors.initials: KM
authors.lastname: Kocan
mesh_terms: D000709:Anaplasma / Q000235:genetics / Q000302:isolation & purification*; D000818:Animals; D015342:DNA Probes; D004269:DNA, Bacterial; D003870:Dermacentor / Q000382:microbiology*; D009693:Nucleic Acid Hybridization; D013987:Ticks / Q000382:microbiology*
chemical_list: D015342:DNA Probes; D004269:DNA, Bacterial