title: Blood plasma and tissue concentrations of vitamin E in beef cattle as influenced by supplementation of various tocopherol compounds.
abstract: Twenty-four crossbred beef cows were used to investigate the concentration of alpha-tocopherol in plasma and tissues following oral administration of four tocopherol sources. Animals were allotted to the following treatments: DL-alpha-tocopherol, D-alpha-tocopherol, DL-tocopheryl acetate and D-alpha-tocopheryl acetate. Animals received a daily oral dose of 1,000 IU of the respective tocopherol treatment for 28 d and then were slaughtered. Blood samples were collected on d 0, 1, 7, 14 and 28 for tocopherol concentration assays, and samples from 10 different tissues were collected from slaughtered cows. Identification of alpha-tocopherol in tissues was confirmed by HPLC retention times and by comparison of mass spectra with that of alpha-tocopherol standards. The D-alpha-tocopherol and its acetate ester increased plasma tocopherol concentration faster than the racemic products, the greatest response occurring with D-alpha-tocopherol. Across all treatments, the highest alpha-tocopherol concentrations were noted in the adrenal gland and liver, the lowest in muscle and thyroid tissue. Tissue analyses confirmed that in adrenal gland, kidney, liver and lung, alpha-tocopherol concentrations were higher following D-alpha than DL-alpha-tocopherol supplementation.
authors.affiliation: Dept. of Anim. Sci., University of Florida, Gainesville 32611.
authors.forename: N
authors.initials: N
authors.lastname: Hidiroglou
authors.forename: L F
authors.initials: LF
authors.lastname: Laflamme
authors.forename: L R
authors.initials: LR
authors.lastname: McDowell
mesh_terms: D000818:Animals; D001682:Biological Availability; D002417:Cattle / Q000378:metabolism*; D005260:Female; D014018:Tissue Distribution; D024505:Tocopherols; D014810:Vitamin E / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000097:blood / Q000493:pharmacokinetics*; D024502:alpha-Tocopherol / Q000031:analogs & derivatives*
chemical_list: D014810:Vitamin E; D024502:alpha-Tocopherol; D024505:Tocopherols