Effects of proximate cholesterol precursors and steroid hormones on mouse myeloma growth in serum-free medium.
The proximate cholesterol precursors lathosterol, 7-dehydrocholesterol and desmosterol supported the growth of NS-1 and X63 mouse myeloma cells. These cells and X63.653 cells are cholesterol auxotrophs, yet each was able to convert [3H]lathosterol to [3H]cholesterol. These results are consistent with the conclusion that cholesterol auxotrophy in these myeloma cells is due to a deficiency in 3-ketosteroid reductase activity. The steroid hormones testosterone, progesterone and hydrocortisone could not replace cholesterol as a medium supplement. These results provide a greater understanding of the cholesterol auxotrophy characteristic of cell lines clonally-derived from the MOPC 21 myeloma tumor, and they provide a rational basis for the use of sterols in defined culture medium for mouse myeloma cells.
Sato
J D
JD
W. Alton Jones Cell Science Center, Inc., Lake Placid, New York 12946.
Cao
H T
HT
Kayada
Y
Y
Cabot
M C
MC
Sato
G H
GH
Okamoto
T
T
Welsh
C J
CJ
D015096:3-Hydroxysteroid Dehydrogenases / Q000378:metabolism; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002775:Cholestadienols / Q000494:pharmacology*; D002784:Cholesterol / Q000378:metabolism / Q000494:pharmacology*; D003470:Culture Media; D003684:Dehydrocholesterols / Q000494:pharmacology*; D003897:Desmosterol / Q000494:pharmacology*; D006728:Hormones / Q000494:pharmacology*; D006854:Hydrocortisone / Q000494:pharmacology; D007536:Isomerism; D051379:Mice; D009101:Multiple Myeloma / Q000378:metabolism / Q000473:pathology*; D011374:Progesterone / Q000494:pharmacology; D013739:Testosterone / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D002775:Cholestadienols; D003470:Culture Media; D003684:Dehydrocholesterols; D006728:Hormones; D003897:Desmosterol; D013739:Testosterone; D011374:Progesterone; C001521:lathosterol; D002784:Cholesterol; C016705:7-dehydrocholesterol; D015096:3-Hydroxysteroid Dehydrogenases; C031298:3-ketosteroid reductase; D006854:Hydrocortisone