Lectin activity and distribution of chicken lactose lectin I in the extracellular matrix of the chick developing kidney.
A lectin activity inhibitable by thiodigalactose, N-acetyllactosamine, lactulose, lactose and by an antibody raised against CLL I (chicken-lactose lectin I) has been investigated in the chick embryo developing kidney. At post-induction stages this activity was found in both mesonephros and metanephros. In immunofluorescence and immunoelectron microscopy, the extracellular distribution of CLL I was similar in the mesonephros and the metanephros. The lectin was never found intracellularly; cultured kidney cells did not express any endogenous lectin but were rich in lectin-receptor sites, which led to the hyphothesis that CLL I is not produced in situ but could be adsorbed on renal cells. Potential physiological roles for embryonic lectins are discussed.
Didier
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E
Laboratoire de Biologie Animale, UA CNRS 677, Université de Clermont-Blaise Pascal, Aubière, France.
Didier
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P
Bayle
D
D
Chevalier
M
M
D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D005109:Extracellular Matrix / Q000032:analysis; D037541:Galectin 4; D006001:Glycoconjugates / Q000378:metabolism; D006388:Hemagglutinins / Q000032:analysis*; D007150:Immunohistochemistry; D007668:Kidney / Q000032:analysis* / Q000196:embryology / Q000378:metabolism; D037102:Lectins / Q000378:metabolism*; D008650:Mesonephros / Q000032:analysis
D037541:Galectin 4; D006001:Glycoconjugates; D006388:Hemagglutinins; D037102:Lectins