Interaction of indomethacin with the antilipolytic effect of prostaglandin E2 in rat adipocytes.
The interaction of indomethacin with the antilipolytic effect of prostaglandin E2 (PGE2) was investigated in rat adipocytes in order to reveal a possible role of endogenous PGs in adipose tissue. Adipocytes isolated from rats treated in vivo for 5 days with indomethacin were compared with non-treated control rats. The sensitivity of the antilipolytic effect of exogenous PGE2 was significantly enhanced in adipocytes from indomethacin-treated rats (IC50 of PGE2: 0.45 +/- 0.05 nM vs. 1.2 +/- 0.1 nM, P less than 0.01). This enhanced antilipolytic effect of exogenous PGE2 could be related to the reduced endogenous PGE2 formation in adipocytes from the indomethacin-treated rats (PGE2 formation was reduced by more than 90%). In agreement with that observation the [p3H]PGE2 receptor binding was enhanced by 58% in adipocytes treated with indomethacin. Thus, indomethacin via inhibition of endogenous PG formation could modulate some properties of lipolysis in adipocytes. However, indomethacin treatment had no significant effects on basal or isoproterenol-stimulated lipolysis.
Richelsen
B
B
Medical Department III, Aarhus Amtssygehus, Denmark.
D000273:Adipose Tissue / Q000166:cytology / Q000187:drug effects*; D000818:Animals; D015232:Dinoprostone / Q000096:biosynthesis / Q000378:metabolism / Q000494:pharmacology*; D007213:Indomethacin / Q000378:metabolism / Q000494:pharmacology*; D008066:Lipolysis / Q000187:drug effects; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D015232:Dinoprostone; D007213:Indomethacin