[Simple kinetic models explaining critical phenomena in enzymatic reactions with isomerization of the enzyme and substrate].
Kinetic models for enzyme reactions are considered which take into account enzyme and substrate isomerization. Application of graph-theoretic methods allows to reveal fragments in schemes which may induce multiple stead-states or concentrational selfoscillations. The role of substrate isomers in the inhibition of enzyme isomers to produce critical phenomena is considered. The boundaries of parameter domains for critical phenomena are estimated. It is shown that the controlled change in concentrations of substrate and enzyme isomers may be important in regulation of enzyme systems, if different enzyme isomers are inhibited mainly by different substrate isomers. The models are used for interpretation of possible critical phenomena in the open reaction catalyzed by lactate dehydrogenase. It is shown that lactate dehydrogenase may act as a trigger in carbohydrate metabolism by changing "critically" its activity in relation to changes in pH and pyruvate fluxes. Slow enzyme inhibition by enolpyruvate is suggested as a possible reason for glycolytic oscillations.
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Ivanova
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D004798:Enzymes / Q000378:metabolism*; D007536:Isomerism; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008433:Mathematics; D008954:Models, Biological; D011487:Protein Conformation; D013379:Substrate Specificity
D004798:Enzymes; D007770:L-Lactate Dehydrogenase
Prostye kineticheskie modeli, ob''iasniaiushchie kriticheskie iavleniia v fermentativnykh reaktsiiakh s izomerizatsieĭ fermenta i substrata.