title: A new technique for lipid analysis using liquid chromatography/mass spectrometry.
abstract: It has been previously reported that ethanol alters the level of polyunsaturated fatty acids, and this may be related to alteration of membrane physical properties. Therefore, developing a technique to efficiently analyze lipid molecular species is of value for alcohol research. A new technique which can provide detailed structural information for most major lipid classes has been developed in our laboratory using thermospray liquid chromatography/mass spectrometry (LC/MS). In this technique, on-line LC separation is achieved with a conventional flow rate and the LC effluent is carried into the mass spectrometer via a heated capillary where it is vaporized and ionized. The results thus obtained for the major lipid classes including fast separation methods and quantitative aspects will be presented.
authors.affiliation: Division of Intramural Clinical and Biological Research, National Institute of Alcohol Abuse and Alcoholism, Bethesda, MD 20892.
authors.forename: H Y
authors.initials: HY
authors.lastname: Kim
authors.forename: N
authors.initials: N
authors.lastname: Salem
mesh_terms: D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D010713:Phosphatidylcholines / Q000032:analysis; D010743:Phospholipids / Q000032:analysis*
chemical_list: D010713:Phosphatidylcholines; D010743:Phospholipids