title: A new chromogenic assay for direct detection of staphylocoagulase.
abstract: Tube test for detection of staphylococcal coagulase, despite of many disadvantages, is commonly used in clinical microbiology for identification of Staphylococcus aureus. In this paper a new chromogenic method for detection of the coagulase directly in staphylococcal cultures is described and evaluated on the basis of a comparison with the standard tube assay. The chromogenic assay appeared to be as sensitive as the tube test but results of the former one can be read in a few hours without any apparatus.
authors.affiliation: Institute of Microbiology, Medial Academy, Kraków, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Bulanda
authors.forename: Z
authors.initials: Z
authors.lastname: Wegrzynowicz
authors.forename: M
authors.initials: M
authors.lastname: Ciurak
authors.forename: J
authors.initials: J
authors.lastname: Kowalczyk
authors.forename: G
authors.initials: G
authors.lastname: Kupryszewski
authors.forename: M
authors.initials: M
authors.lastname: Gruszka
authors.forename: P B
authors.initials: PB
authors.lastname: Heczko
authors.forename: G
authors.initials: G
authors.lastname: Pulverer
mesh_terms: D002863:Chromogenic Compounds; D003030:Coagulase / Q000032:analysis*; D003124:Colorimetry; D003470:Culture Media; D006801:Humans; D011237:Predictive Value of Tests; D013211:Staphylococcus aureus / Q000201:enzymology*
chemical_list: D002863:Chromogenic Compounds; D003030:Coagulase; D003470:Culture Media