title: Purification of human 6-phosphogluconate dehydrogenase from human haemolysate with chromatography on an immobilized dye as the essential step and use of automation. Simultaneous purification of lactate dehydrogenase.
abstract: The screening procedure described in the preceding paper allowed a practical purification procedure to be devised that was automated for human 6-phosphogluconate dehydrogenase. The purification needed only two chromatographic steps, first on immobilized Procion Blue HE-GN and then on Phenyl-Sepharose. This technique also gave purified lactate dehydrogenase. Both enzymes showed single bands in SDS polyacrylamide gel electrophoresis.
authors.affiliation: INSERM U160, Hôpital Beaujon, Clichy, France.
authors.forename: Y
authors.initials: Y
authors.lastname: Kroviarski
authors.forename: S
authors.initials: S
authors.lastname: Cochet
authors.forename: C
authors.initials: C
authors.lastname: Vadon
authors.forename: A
authors.initials: A
authors.lastname: Truskolaski
authors.forename: P
authors.initials: P
authors.lastname: Boivin
authors.forename: O
authors.initials: O
authors.lastname: Bertrand
mesh_terms: D002021:Buffers; D002852:Chromatography, Ion Exchange; D004396:Coloring Agents; D004591:Electrophoresis, Polyacrylamide Gel; D006461:Hemolysis; D006801:Humans; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000097:blood* / Q000302:isolation & purification; D009711:Nucleotides / Q000302:isolation & purification; D010734:Phosphogluconate Dehydrogenase / Q000097:blood* / Q000302:isolation & purification
chemical_list: D002021:Buffers; D004396:Coloring Agents; D007527:Isoenzymes; D009711:Nucleotides; D007770:L-Lactate Dehydrogenase; D010734:Phosphogluconate Dehydrogenase