title: Exercise-induced changes in platelet aggregation; a comparison of whole blood and platelet rich plasma techniques.
abstract: Studies have been performed to assess the effect of exercise on spontaneous platelet aggregation in shaken whole blood, and on agonist-induced platelet aggregation in whole blood and platelet rich plasma (PRP). Spontaneous platelet aggregation in shaken whole blood was increased following exercise compared to pre-exercise values. The increase in spontaneous aggregation after exercise correlated inversely with the increase in white cell count in whole blood. Platelet sensitivity in whole blood to adrenaline, collagen and adenosine diphosphate (ADP) was increased following exercise. Changes in platelet sensitivity to adrenaline following exercise correlated with increases in plasma noradrenaline levels but not with changes in blood cell counts. In PRP, platelet sensitivity to ADP and to collagen was increased following exercise when the pre and post-exercise PRP platelet counts were not corrected to allow for the increase in platelet count which occurred with exercise. When the PRP platelet counts were corrected, no changes in platelet sensitivity to any agonist after exercise were observed.
authors.affiliation: Academic Unit of Diabetes and Endocrinology, University College, London, U.K.
authors.forename: T J
authors.initials: TJ
authors.lastname: Hendra
authors.forename: J
authors.initials: J
authors.lastname: Oughton
authors.forename: C C
authors.initials: CC
authors.lastname: Smith
authors.forename: D J
authors.initials: DJ
authors.lastname: Betteridge
authors.forename: J S
authors.initials: JS
authors.lastname: Yudkin
mesh_terms: D000244:Adenosine Diphosphate / Q000494:pharmacology; D000328:Adult; D001772:Blood Cell Count; D003094:Collagen / Q000494:pharmacology; D004837:Epinephrine / Q000494:pharmacology; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D009638:Norepinephrine / Q000097:blood; D005082:Physical Exertion; D010949:Plasma / Q000166:cytology / Q000502:physiology; D010974:Platelet Aggregation / Q000187:drug effects; D013312:Stress, Physiological / Q000097:blood
chemical_list: D000244:Adenosine Diphosphate; D003094:Collagen; D009638:Norepinephrine; D004837:Epinephrine