Kinetics of intraventricularly injected trace amines and their deuterated isotopomers.
Intraventricular injection into the rat brain of four trace amines and a catecholamine resulted in rapid exponential loss of the amines in the first 30 minutes after injection. The half-lives were: phenylethylamine 3.8 min, para-tyramine 5.1 min, meta-tyramine 7.4 min and dopamine 8.0 min. Tryptamine showed a biphasic loss with half-lives of 4.7 min (over the 5 to 10 min period) and 14.1 min (10 to 30 min). The half-lives were substantially increased by deuterium labeling at the alpha carbon position: phenylethylamine 4.8 min, para-tyramine 8.8 min, meta-tyramine 14.1 min, dopamine 13.0 min and tryptamine 6.0 min (5 to 10 min period) and 28.7 min (10 to 20 min). The loss of the amines was reduced by monoamine oxidase inhibition by pargyline hydrochloride and the deuterium isotope effect was abolished. It is noteworthy that the half-life of dopamine was similar to those of the trace amines in this time period and that the trace amine half-lives after i.v. injection was longer than those obtained from measurements of increases of concentrations of endogenous amines after MAOI in vivo and that of dopamine shorter than values calculated from turnover measurements.
Durden
D A
DA
Neuropsychiatric Research Unit, University of Saskatchewan, Saskatoon.
Nguyen
T V
TV
Boulton
A A
AA
D000818:Animals; D001679:Biogenic Amines / Q000493:pharmacokinetics*; D001921:Brain / Q000378:metabolism*; D003903:Deuterium; D004298:Dopamine / Q000378:metabolism; D007276:Injections, Intraventricular; D008297:Male; D010627:Phenethylamines / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D014363:Tryptamines / Q000378:metabolism; D014439:Tyramine / Q000378:metabolism
D001679:Biogenic Amines; D010627:Phenethylamines; D014363:Tryptamines; C030820:tryptamine; D003903:Deuterium; D004298:Dopamine; D014439:Tyramine