Nucleotide sequence and tissue distribution of the human mitochondrial aspartate aminotransferase mRNA.
The cDNA of human mitochondrial aspartate aminotransferase (E.C.2.6.1.1.) was isolated from a human liver cDNA library using a rat mitochondrial aspartate aminotransferase cDNA as probe. The sequence of this cDNA gives a predicted aminoacid sequence for the human presequence and for the human mature protein exhibiting respectively 93% and 95% homology with rat sequences. A Northern blot of total RNA, isolated from various human tissues and hybridized with this cDNA, revealed a single 2.4 Kb RNA band. Mitochondrial aspartate aminotransferase RNA was clearly detected in human kidney, placenta, stomach and spleen as well as in both fetal and adult liver.
Pol
S
S
Inserm U-99, Hôpital Henri Mondor, Creteil, France.
Bousquet-Lemercier
B
B
Pave-Preux
M
M
Pawlak
A
A
Nalpas
B
B
Berthelot
P
P
Hanoune
J
J
Barouki
R
R
D000595:Amino Acid Sequence; D000818:Animals; D001219:Aspartate Aminotransferases / Q000235:genetics*; D001483:Base Sequence; D015152:Blotting, Northern; D003062:Codon; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D006801:Humans; D007668:Kidney / Q000201:enzymology; D008099:Liver / Q000196:embryology / Q000201:enzymology; D008928:Mitochondria / Q000201:enzymology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010920:Placenta / Q000201:enzymology; D011247:Pregnancy; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics*; D051381:Rats; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D013154:Spleen / Q000201:enzymology; D013270:Stomach / Q000201:enzymology
D003062:Codon; D012333:RNA, Messenger; D004247:DNA; D001219:Aspartate Aminotransferases