[Selective incorporation of polyunsaturated fatty acids of the omega-3 family in the diet into the cerebellar phospholipids of rats].
TLC, GLC and GC-MS methods were applied for studying the fatty acid composition of rat cerebellum phospholipids. 75 male Wistar rats had a diet without fat for 6 weeks and then a diet with linseed oil, fish oil in combination with lard and mixture of sunflower oil with lard (control group) for 3 months; PUFA omega 6/omega 3 rations were 0.27; 0.5 and 45.0. PUFA omega 3 in all fractions were represented mainly by 22:6 omega 3 (0.2-12.0%) acid and its share depends on the total PUFA omega 3 amount in the diet. The absence of 18:3, 20:5 and 22:5 omega 3 in cerebellum lipids, fed in great amount with test diets, suggested that these acids are rapidly desaturated and elongated to 22:6 omega 3 at the blood-brain barrier. Established competitive inhibition of desaturases responsible for the synthesis of tissues PUFA omega 6 and omega 3 proves the expressed regulatory effect of alimentary factor on brain lipids.
Medvedev
F A
FA
Kulakova
S N
SN
Levachev
M M
MM
D000818:Animals; D001812:Blood-Brain Barrier; D001923:Brain Chemistry; D002531:Cerebellum / Q000032:analysis / Q000378:metabolism*; D002849:Chromatography, Gas; D004042:Dietary Fats, Unsaturated / Q000032:analysis / Q000378:metabolism*; D005231:Fatty Acids, Unsaturated / Q000032:analysis / Q000378:metabolism*; D008297:Male; D010743:Phospholipids / Q000032:analysis / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D004042:Dietary Fats, Unsaturated; D005231:Fatty Acids, Unsaturated; D010743:Phospholipids
Izbiratel'noe vkliuchenie polinenasyshchennykh zhirnykh kislot semeĭstva omega 3 ratsiona v fosfolipidy mozzhechka krys.