title: Vimentin expression in oocytes, eggs and early embryos of Xenopus laevis.
abstract: Immunocytochemical studies using a monoclonal anti-porcine vimentin antibody reveal a well-organized pattern of staining in Xenopus laevis oocytes, eggs and early embryos. The positions of Xenopus vimentin and desmin in two-dimensional (2D) polyacrylamide gels were first established by immunoblotting of muscle Triton extracts with anti-intermediate filament antibodies (anti-IFA), which cross-react with all intermediate filament proteins (IFPs). The anti-porcine vimentin reacts with vimentin and desmin in muscle 2D immunoblots, but only reacts with one polypeptide in oocyte blots in the position predicted for vimentin (Mr 55 x 10(3), pI 5.6). Using an anti-sense probe derived from a Xenopus vimentin genomic clone in RNase protection assays, we show that expression of vimentin begins in previtellogenic oocytes. The level of expression remains constant throughout oogenesis and in unfertilized eggs. These data suggest that vimentin is expressed in oocytes and eggs. Most interestingly, the immunocytochemical results also show that vimentin is present in the germ plasma of oocytes, eggs and early embryos. It is therefore possible that vimentin has an important role in the formation or behaviour of early germ line cells.
authors.affiliation: Department of Anatomy, St. George's Hospital Medical School, London, UK.
authors.forename: P
authors.initials: P
authors.lastname: Tang
authors.forename: C R
authors.initials: CR
authors.lastname: Sharpe
authors.forename: T J
authors.initials: TJ
authors.lastname: Mohun
authors.forename: C C
authors.initials: CC
authors.lastname: Wylie
mesh_terms: D000818:Animals; D003599:Cytoskeleton / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D004625:Embryo, Nonmammalian / Q000032:analysis*; D005260:Female; D006651:Histocytochemistry; D015151:Immunoblotting; D007382:Intermediate Filaments / Q000032:analysis*; D009865:Oocytes / Q000032:analysis*; D010063:Ovum / Q000032:analysis*; D012260:Ribonucleases; D014746:Vimentin / Q000032:analysis*; D014982:Xenopus laevis
chemical_list: D014746:Vimentin; D012260:Ribonucleases