title: The effect of a pulsed neodymium-YAG laser on chorioretinal monolayered cell cultures.
abstract: The effect of a pulsed YAG laser on monolayers of chorioretinal cells maintained in vitro on plastic surface was examined. At the energy levels used, phase contrast microscopic visible lesions were observed at all focus distances up to 2 mm. Scanning electron microscopy demonstrated curling of detached sheets of cells, displacement of single cells, clustering of cells, and severe cell membrane damage at the edge of the lesions. The present system permits evaluation of the effect of different laser characteristics and application modes on the extent and morphology of damage in monolayers of human cells under easily controlled conditions.
authors.affiliation: Department of Ophthalmology, Ullevål Hospital, Oslo, Norway.
authors.forename: N P
authors.initials: NP
authors.lastname: Lilledahl
authors.forename: B
authors.initials: B
authors.lastname: Nicolaissen
authors.forename: C
authors.initials: C
authors.lastname: Allen
authors.forename: K
authors.initials: K
authors.lastname: Beraki
authors.forename: R J
authors.initials: RJ
authors.lastname: Ullshafer
mesh_terms: D002478:Cells, Cultured; D002829:Choroid / Q000473:pathology* / Q000648:ultrastructure; D006801:Humans; D053685:Laser Therapy / Q000009:adverse effects*; D008855:Microscopy, Electron, Scanning; D009354:Neodymium; D012160:Retina / Q000473:pathology* / Q000648:ultrastructure
chemical_list: D009354:Neodymium