Dual effect of N-methyl-N-(1-methyl-4-pyrolidino)-2-butyl)acetamide on release of (3H)-acetylcholine from the rat hippocampal slices.
The effect of muscarinic cholinergic drugs on (3H)-acetylcholine [3H)-Ach) release from slices of rat hippocampus was investigated either in the presence of eserine or hemicholinium-3 (HC-3), 10 microM each. BM-5 (N-methyl-N-(1-methyl-4-pyrolidino-2-butyl)acetamide) is a partial muscarinic cholinergic agonist. Like oxotremorine, BM-5 significantly (p less than 0.012) decreased the release of (3H)-Ach in the presence of HC-3. In the presence of eserine, (3H)-Ach release was significantly (p less than 0.001) enhanced both by atropine and BM-5. The decrease or increase in release of (3H)-Ach by BM-5, in the presence of HC-3 or eserine, respectively, may be due to its partial agonist effect on hippocampal muscarinic cholinergic receptors.
Sethy
V H
VH
CNS Research, Upjohn Company, Kalamazoo, MI 49001.
Francis
J W
JW
Russell
R R
RR
Ruppel
P L
PL
D000109:Acetylcholine / Q000378:metabolism*; D000818:Animals; D001285:Atropine / Q000494:pharmacology; D006624:Hippocampus / Q000187:drug effects*; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D010095:Oxotremorine / Q000494:pharmacology; D010277:Parasympathomimetics / Q000494:pharmacology*; D011759:Pyrrolidines / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D011976:Receptors, Muscarinic / Q000187:drug effects
D010277:Parasympathomimetics; D011759:Pyrrolidines; D011976:Receptors, Muscarinic; D010095:Oxotremorine; D001285:Atropine; C038672:N-methyl-N-(1-methyl-4-pyrrolidino-2-butynyl)acetamide; D000109:Acetylcholine