Induction of contact photosensitivity to TCSA using photohapten-modified syngeneic spleen cells.
We investigated the induction and transfer of contact photosensitivity (CPS) to the photohapten 3,3',4',5-tetrachlorosalicylanilide (TCSA) using photo-TCSA-coupled syngeneic cells in mice. PhotoTCSA-modified spleen cells (photoTCSA-SC) with efficient immunogenicity were prepared with ultraviolet A (UVA) irradiation of spleen cells suspended in TCSA solution. Subcutaneous inoculation of photoTCSA-SC into syngeneic mice induced a highly specific CPS response detected by ear swelling upon epicutaneous challenge with TCSA painting plus UVA irradiation. The sensitivity was determined to be a cell-mediated, delayed-type hypersensitivity reaction from the time course of the reactivity, the characteristic histology, and the successful transfer of the sensitivity into syngeneic naive recipients by immune lymph node T cells with the phenotype of L3T4+, Lyt-2-. In contrast to the conventional TCSA painting plus UVA irradiation method, this immunization procedure did not evoke an ordinary contact sensitivity reaction to TCSA. The present procedure represented a new way to induce CPS.
Tokura
Y
Y
Department of Dermatology, Hamamatsu University School of Medicine, Japan.
Takigawa
M
M
Yamada
M
M
D000818:Animals; D006241:Haptens / Q000528:radiation effects; D007116:Immunization, Passive; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010777:Photochemistry; D010787:Photosensitivity Disorders / Q000209:etiology*; D012457:Salicylamides / Q000633:toxicity*; D012458:Salicylanilides / Q000276:immunology / Q000528:radiation effects / Q000633:toxicity*; D013154:Spleen / Q000276:immunology / Q000528:radiation effects; D014466:Ultraviolet Rays
D006241:Haptens; D012457:Salicylamides; D012458:Salicylanilides; C003390:3,3',4',5-tetrachlorosalicylanilide