title: Enzymatic amplification and characterization of large DNA fragments from genomic DNA.
abstract: Conditions for DNA amplification in vitro using modified T7 DNA polymerase have been devised to obtain 2000-bp DNA fragments of the HGPRT gene directly from human genomic DNA. The DNA obtained from a 1.2 x 10(5)-fold amplification has been used for direct sequencing.
authors.affiliation: MIT Center for Environmental Health Sciences, Massachusetts Institute of Technology, Cambridge 02139.
authors.forename: P
authors.initials: P
authors.lastname: Keohavong
authors.forename: C C
authors.initials: CC
authors.lastname: Wang
authors.forename: R S
authors.initials: RS
authors.lastname: Cha
authors.forename: W G
authors.initials: WG
authors.lastname: Thilly
mesh_terms: D001483:Base Sequence; D004261:DNA Replication; D004259:DNA-Directed DNA Polymerase; D005784:Gene Amplification; D006801:Humans; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics; D009838:Oligodeoxyribonucleotides
chemical_list: D009838:Oligodeoxyribonucleotides; D007041:Hypoxanthine Phosphoribosyltransferase; D004259:DNA-Directed DNA Polymerase