Fast and easy detection of mouse sex chimeras using electrophoretic polymorphism of phosphoglycerate kinase-1, an X chromosome-linked enzyme.
About half of the chimeras produced by aggregation of two mouse embryos are sex chimeras composed of both XX and XY cells. We developed a fast and easy method to identify sex chimeras by using electrophoretic bimorphism of an X-linked enzyme, phosphoglycerate kinase-1 (PGK-1), as a marker. When embryos resulting from the crossing of a Pgk-1b/Pgk-1b female and a Pgk-1a/Y male are aggregated, the genotype of sex chimeras is Pgk-1b/Pgk-1a----Pgk-1b/Y. Most of these were identifiable from the PGK-1 electrophoretic pattern of blood cells (i.e., AB type) and the appearance of genitalia (male type or apparently abnormal). Genotypes of functional sperm in the testes of the male-type sex chimeras were also identifiable from the PGK-1 electrophoretic pattern of progenies. Examination of gonads of the sex chimeras revealed that a considerable proportion was hermaphorditic. With this method, reasonable numbers of male-type sex chimeras and hermaphrodites may be selected and used as material for investigating sexual differentiation.
Nakayama
H
H
Division of Cancer Pathology, Biomedical Research Center, Osaka University Medical School, Japan.
Kuroda
H
H
Ru
X M
XM
Fujita
J
J
Kitamura
Y
Y
D000818:Animals; D002678:Chimera; D012734:Disorders of Sex Development / Q000235:genetics* / Q000473:pathology; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D008040:Genetic Linkage; D005838:Genotype; D006066:Gonads / Q000473:pathology; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008813:Mice, Inbred ICR; D010735:Phosphoglycerate Kinase / Q000032:analysis / Q000235:genetics*; D011110:Polymorphism, Genetic; D014960:X Chromosome
D010735:Phosphoglycerate Kinase