Isolation of clones from T24 cells and test of their sensitivity to adriamycin.
Two single cell clones, CL3 and CL7, were developed from T24 cells. CL3 had abundant cytoplasmic granules, while CL7 did not have as much. CL3 and CL7 exhibited a modal number of chromosomes at 69 and 74, respectively. CL7 had a higher growth rate than CL3, as indicated by in vitro cell growth, plating efficiency, and growth of the tumors produced in nude mice. CL3 was slightly susceptible to adriamycin toxicity as compared to CL7.
Tsushima
T
T
Dept. of Urology, Okayama University Medical School, Japan.
Tokiwa
T
T
Matsumura
Y
Y
Yoshimoto
J
J
Obama
T
T
Ohmori
H
H
Sato
J
J
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002999:Clone Cells; D004317:Doxorubicin / Q000494:pharmacology*; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D007621:Karyotyping; D051379:Mice; D008819:Mice, Nude; D014183:Transplantation, Heterologous; D001749:Urinary Bladder Neoplasms / Q000235:genetics / Q000473:pathology
D004317:Doxorubicin