title: Immunopotentiation by orally-administered Quillaja saponins: effects in mice vaccinated intraperitoneally against rabies.
abstract: Orally fed Quillaja saponins amplified the immunopotentiating ability of an intraperitoneally (IP) administered inactivated rabies vaccine in mice. The number of animals surviving rabies infection was markedly higher (90-100%) in groups of animals receiving a combined treatment of oral saponin (SAP) and IP vaccine, compared to groups receiving vaccine alone (25%), or to unimmunized mice (0%). Antibody production was significantly higher in animals fed SAP 2 weeks after primary or secondary sensitization with an IP-injected vaccine. In mice given 2 IP doses of vaccine, 1 week apart, simultaneous feeding of SAP resulted in an enhanced production of rabies-specific (whole Ig) antibodies. On the other hand, animals preconditioned with SAP 3 days prior to administration of the vaccine exhibited greatly increased IgG antibody levels. Moreover, SAP-preconditioned mice vaccinated with a very low dosage produced significantly higher levels of antibodies.
authors.affiliation: Department of Microbiology, Faculty of Medicine, University of Toronto, Canada.
authors.forename: S R
authors.initials: SR
authors.lastname: Chavali
authors.forename: L D
authors.initials: LD
authors.lastname: Barton
authors.forename: J B
authors.initials: JB
authors.lastname: Campbell
mesh_terms: D000276:Adjuvants, Immunologic; D000284:Administration, Oral; D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D007274:Injections, Intraperitoneal; D051379:Mice; D008815:Mice, Inbred Strains; D011818:Rabies / Q000276:immunology / Q000517:prevention & control; D011819:Rabies Vaccines / Q000008:administration & dosage / Q000276:immunology*; D012503:Saponins / Q000008:administration & dosage / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D011819:Rabies Vaccines; D012503:Saponins