title: Time-resolved fluoroimmunoassay of cortisol in serum with a europium chelate as label.
abstract: A non-isotopic heterogeneous competitive immunoassay of serum cortisol is described. Cortisol present in the sample competes with immobilised cortisol (cortisol-thyroglobulin conjugate) for binding to a monoclonal anti-cortisol biotinylated antibody. The amount of antibody bound is measured on the dry solid-phase by time-resolved fluorometry after adding streptavidin labeled with the Eu3+ chelate 4,7-bis(chlorosulfophenyl)-1,10 phenanthroline-2,9-dicarboxylic acid (BCPDA), in the presence of excess Eu3+. The assay is simple to perform, its characteristics are similar to those of radioimmunoassay techniques, and is suitable for routine clinical use.
authors.affiliation: CyberFluor Inc., Toronto, Ontario, Canada.
authors.forename: E P
authors.initials: EP
authors.lastname: Diamandis
authors.forename: V
authors.initials: V
authors.lastname: Bhayana
authors.forename: K
authors.initials: K
authors.lastname: Conway
authors.forename: E
authors.initials: E
authors.lastname: Reichstein
authors.forename: A
authors.initials: A
authors.lastname: Papanastasiou-Diamandis
mesh_terms: D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins; D002614:Chelating Agents; D005063:Europium; D015200:Fluoroimmunoassay / Q000379:methods; D006854:Hydrocortisone / Q000097:blood*; D010618:Phenanthrolines; D019809:Streptavidin; D013954:Thyroglobulin
chemical_list: D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins; D002614:Chelating Agents; D010618:Phenanthrolines; C054206:4,7-bis(chlorosulfophenyl)-1,10-phenanthroline-2,9-dicarboxylic acid; D005063:Europium; D013954:Thyroglobulin; D019809:Streptavidin; D006854:Hydrocortisone