Effect of drugs in vitro on lysosomal enzyme activities in bovine retinal pigment epithelial cells.
We examined in vitro the effect of several drugs on the activities of lysosomal enzymes in the crude extract of bovine retinal pigment epithelial cells. Acid phosphatase, beta-D-glucuronidase, N-acetyl-beta-D-glucosaminidase, alpha-L-fucosidase, and alpha-D-mannosidase were used as lysosomal enzymes. Sodium iodate at 10(-6), 10(-5) and 10(-4) M, and potassium iodate at 10(-5) and 10(-4) M inhibited acid phosphatase activity. Ferrous chloride at 10(-5) and 10(-4) M suppressed beta-D-glucuronidase activity. Ferric chloride, indomethacin, chloroquine, chlorpromazine, 5-fluorouracil, tobramycin, daunomycin, oxalate and epinephrine had no or only minimal inhibitory effects on the lysosomal enzyme activities examined.
Hayasaka
S
S
Department of Ophthalmology, Shimane Medical University, Izumo, Japan.
Noda
S
S
Setogawa
T
T
D000818:Animals; D002417:Cattle; D005296:Ferrous Compounds / Q000494:pharmacology; D066298:In Vitro Techniques; D007452:Iodates / Q000494:pharmacology; D008247:Lysosomes / Q000201:enzymology*; D010857:Pigment Epithelium of Eye / Q000187:drug effects* / Q000201:enzymology; D011188:Potassium / Q000494:pharmacology; D017680:Potassium Compounds
D005296:Ferrous Compounds; D007452:Iodates; D017680:Potassium Compounds; C039693:potassium iodate; D011188:Potassium; C029451:ferrous chloride; C032285:sodium iodate