title: Cultivation of exoerythrocytic stages of Plasmodium cynomolgi, P. knowlesi, P. coatneyi, and P. inui in Macaca mulatta hepatocytes.
abstract: Exoerythrocytic stage parasites of Plasmodium cynomolgi, P. knowlesi, P. coatneyi, and P. inui were cultured by inoculating primary cultures of hepatocytes from Macaca mulatta with sporozoites. Less than 1% of inoculated sporozoites survived. Morphology and size of the liver stages in all 4 species were similar to in vivo descriptions and the time required for in vitro maturation correlated well with prepatent periods described.
authors.affiliation: Malaria Branch, Center for Infectious Diseases, U.S. Department of Health and Human Services, Atlanta, Georgia 30333.
authors.forename: P
authors.initials: P
authors.lastname: Millet
authors.forename: T L
authors.initials: TL
authors.lastname: Fisk
authors.forename: W E
authors.initials: WE
authors.lastname: Collins
authors.forename: J R
authors.initials: JR
authors.lastname: Broderson
authors.forename: P
authors.initials: P
authors.lastname: Nguyen-Dinh
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D008099:Liver / Q000166:cytology / Q000469:parasitology*; D008253:Macaca mulatta; D010961:Plasmodium / Q000254:growth & development*