A method for parallel determination of choline acetyltransferase and muscarinic cholinergic receptors: application in aged-rat brain.
We have devised a method for the parallel determination of choline acetyltransferase (CAT) and muscarinic cholinergic receptor (mCh-R) in the same brain tissue. The method for CAT activity determination is more rapid, simplified, stable, and economical than the usual Fonnum's method. With our method, age-associated changes in CAT activity and mCh-R levels were examined. Although CAT activity hardly changed with age except in a few areas, mCh-R binding of aged-rats was markedly reduced in all areas. These results suggest that the change in mCh-R represents an age-associated biochemical change in the brain and that determination of CAT activity is not sufficient for the study of age-associated changes in the brain cholinergic system.
Haba
K
K
Department of Neurochemistry, Okayama University Medical School, Japan.
Ogawa
N
N
Kawata
M
M
Mori
A
A
D000375:Aging / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000254:growth & development / Q000378:metabolism*; D002795:Choline O-Acetyltransferase / Q000378:metabolism*; D008297:Male; D009448:Neurochemistry / Q000379:methods*; D051381:Rats; D011919:Rats, Inbred Strains; D011976:Receptors, Muscarinic / Q000378:metabolism*
D011976:Receptors, Muscarinic; D002795:Choline O-Acetyltransferase