Preparation of human hemoglobin Ao for possible use as a blood substitute.
A procedure is presented for the preparation of a purified fraction of adult human hemoglobin (HbAo) from one unit of outdated blood. The entire process requires less than 16 h and gives a sterile, endotoxin-free solution of HbAo (approximately 30 g) in a yield of 50%. The solutions are isoionic with a conductivity of less than 15 mu mhos and less than 2 mmol total phosphorus per mol heme. The methemoglobin content is less than 1% and on storage at 4 degrees C rises less than 1% per month.
Christensen
S M
SM
Letterman Army Institute of Research, Blood Research Division, Presidio of San Francisco, CA 94129-6800.
Medina
F
F
Winslow
R W
RW
Snell
S M
SM
Zegna
A
A
Marini
M A
MA
D000328:Adult; D001773:Blood Cells; D001802:Blood Substitutes; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D006441:Hemoglobin A / Q000302:isolation & purification*; D006461:Hemolysis; D006801:Humans; D007525:Isoelectric Focusing
D001802:Blood Substitutes; C028881:hemoglobin A(0); D006441:Hemoglobin A