Time-resolved fluoroimmunoassay of cortisol in serum with a europium chelate as label.
A non-isotopic heterogeneous competitive immunoassay of serum cortisol is described. Cortisol present in the sample competes with immobilised cortisol (cortisol-thyroglobulin conjugate) for binding to a monoclonal anti-cortisol biotinylated antibody. The amount of antibody bound is measured on the dry solid-phase by time-resolved fluorometry after adding streptavidin labeled with the Eu3+ chelate 4,7-bis(chlorosulfophenyl)-1,10 phenanthroline-2,9-dicarboxylic acid (BCPDA), in the presence of excess Eu3+. The assay is simple to perform, its characteristics are similar to those of radioimmunoassay techniques, and is suitable for routine clinical use.
Diamandis
E P
EP
CyberFluor Inc., Toronto, Ontario, Canada.
Bhayana
V
V
Conway
K
K
Reichstein
E
E
Papanastasiou-Diamandis
A
A
D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins; D002614:Chelating Agents; D005063:Europium; D015200:Fluoroimmunoassay / Q000379:methods; D006854:Hydrocortisone / Q000097:blood*; D010618:Phenanthrolines; D019809:Streptavidin; D013954:Thyroglobulin
D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins; D002614:Chelating Agents; D010618:Phenanthrolines; C054206:4,7-bis(chlorosulfophenyl)-1,10-phenanthroline-2,9-dicarboxylic acid; D005063:Europium; D013954:Thyroglobulin; D019809:Streptavidin; D006854:Hydrocortisone