Bone cell populations and histomorphometric correlates to function.
A circadian context has been used to develop information about the proliferative and functional behavior of the cell populations that function to model the long bones of growing rats. We asked: Are the proliferating cells in the growth cartilages and diaphyseal bone of young adult growing rats distributed within single or multiple populations? Can cytomorphometry (TEM-C) be used to determine ultrastructural correlates to the well-defined circadian rhythm of matrix formation displayed by functionally synchronous populations of metaphyseal osteoblasts? Can TEM-C reveal changes in osteoclast ultrastructure that could index a biological rhythm for osteoclastic bone mineralysis/resorption? Kinetic results derived from multiple radiothymidine labeling (DNA synthesis) support the single population model for chondrocytes and diaphyseal osteoprogenitor cells. TEM-C studies at the midpoints of the daily light and dark spans show that osteoblast RER-membrane development and cysternal volumes are maximal at the recorded daytime peak of net collagen synthesis. The extent of metaphyseal osteoclast surface ruffling (mineralysis) is also twofold greater during the day than the night--an observation supporting the concept that bone formation and resorption activities are coupled.
Simmons
D J
DJ
Department of Surgery, University of Texas Medical Branch, Galveston 77550.
Menton
D N
DN
Russell
J E
JE
Smith
R
R
Walker
W V
WV
D000818:Animals; D001842:Bone and Bones / Q000166:cytology* / Q000502:physiology; D002940:Circadian Rhythm; D003094:Collagen / Q000096:biosynthesis; D004247:DNA / Q000096:biosynthesis; D004721:Endoplasmic Reticulum / Q000648:ultrastructure; D006056:Golgi Apparatus / Q000648:ultrastructure; D006132:Growth Plate / Q000166:cytology / Q000378:metabolism / Q000502:physiology; D008297:Male; D008854:Microscopy, Electron; D010006:Osteoblasts / Q000378:metabolism / Q000648:ultrastructure; D010010:Osteoclasts / Q000378:metabolism; D051381:Rats
D003094:Collagen; D004247:DNA