title: Nuclear DNA content in prostatic carcinoma measured by flow cytometry: a retrospective study on paraffin-embedded tissue.
abstract: The nuclear DNA content in 67 prostatic carcinomas was measured by flow cytometry of nuclear suspensions prepared from paraffin-embedded tissue blocks. A method was developed that provided nuclear suspensions of good quality whether tissue blocks from transurethral resections or transvesical prostatectomies were used. Thirty (43.7%) of the tumors were found to be aneuploid. No significant correlation between clinical stage or histological grade and aneuploidy was demonstrated.
authors.affiliation: Dept. of Surgery A, Central Hospital, Hillerød, Denmark.
authors.forename: H
authors.initials: H
authors.lastname: Willumsen
authors.forename: J
authors.initials: J
authors.lastname: Thorup
authors.forename: T
authors.initials: T
authors.lastname: Nørgaard
authors.forename: O H
authors.initials: OH
authors.lastname: Hansen
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000782:Aneuploidy; D002277:Carcinoma / Q000235:genetics*; D002467:Cell Nucleus / Q000032:analysis; D004273:DNA, Neoplasm / Q000032:analysis*; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D008875:Middle Aged; D011471:Prostatic Neoplasms / Q000235:genetics*; D012189:Retrospective Studies
chemical_list: D004273:DNA, Neoplasm