Comparison of paraquat-specific murine monoclonal antibodies produced by in vitro and in vivo immunization.
The techniques of in vitro and in vivo immunization have been used to produce murine hybridomas secreting anti-paraquat IgM and IgG antibodies, respectively. The monoclonal antibodies from these IgM-secreting and IgG-secreting cell lines have been purified by HPLC and characterized by ELISA for their affinities to eliciting antigen and cross-reactivity. Both antibody preparations exhibited high selectivity and similar affinities for paraquat. There was only a minor degree of interaction with a range of paraquat analogs that differed in the nature and position of group substituents.
Johnston
S C
SC
Department of Medicine, University of Queensland, Princess Alexandra Hospital, Brisbane, Australia.
Bowles
M
M
Winzor
D J
DJ
Pond
S M
SM
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000302:isolation & purification; D000918:Antibody Specificity; D002459:Cell Fusion; D002851:Chromatography, High Pressure Liquid; D003001:Cloning, Molecular; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D007114:Immunization; D007074:Immunoglobulin G / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D010269:Paraquat / Q000276:immunology*; D013950:Thymus Gland / Q000166:cytology / Q000187:drug effects
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D010269:Paraquat