Liquid chromatographic determination of penicillins by postcolumn degradation with sodium hypochlorite using an hollow-fibre membrane reactor.
A sensitive, high-performance liquid chromatographic method involving postcolumn degradation with sodium hypochlorite and using a hollow-fibre membrane as a reactor is described for the determination of penicillins. Penicillins were separated on a C18 column followed by postcolumn reaction with sodium hypochlorite and sodium hydroxide using aminated and sulphonated hollow-fibre membrane reactors immersed in each solution, and detected at 270-280 nm based on the UV absorbances of the degradation products. At penicillin concentrations of 2 micrograms/ml, the precisions (relative standard deviation) were 2.28-4.78%. The detection limits of the proposed method were 2.5-25 ng for each penicillin at a signal-to-noise ratio of 3. Ampicillin and its metabolites [(5R,6R)-ampicilloic acid, the (5S,6R)-epimer and (2R)-pierazine-2',5'-dione] in human serum and urine were simultaneously determined by this method.
Haginaka
J
J
Faculty of Pharmaceutical Sciences, Mukogawa Women's University, Hyogo, Japan.
Wakai
J
J
Nishimura
Y
Y
Yasuda
H
H
D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007202:Indicators and Reagents; D010406:Penicillins / Q000032:analysis* / Q000097:blood / Q000652:urine; D012973:Sodium Hypochlorite; D013056:Spectrophotometry, Ultraviolet
D007202:Indicators and Reagents; D010406:Penicillins; D012973:Sodium Hypochlorite