(1----5)-linked beta-D-galactofuranosides are immunodominant in extracellular polysaccharides of Penicillium and Aspergillus species.
Aspergillus and Penicillium species produce extracellular polysaccharides which are immunologically active. Methyl beta-D-galactofuranoside interferes with the reaction between the polysaccharide antigens and the antibodies raised in rabbits. Of the different interlinked dimers of beta-D-galactofuranosides (1----2; 1----3; 1----5; 1----6) the (1----5) interlinked beta-D-galactofuranoside gave the highest inhibition. An increasing inhibitory effect of di-, tri-, tetra-, penta-, hexa-, and heptamer of (1----5) interlinked beta-D-galactofuranosides was observed. It was noticed that the penta-, hexa- and heptamer of (1----5) interlinked beta-D-galactofuranosides were able to link antibodies raised against the extracellular polysaccharides produced by Penicillium species. The tetramer molecule was able to neutralize the binding of antibodies, which are naturally present in human sera, to the polysaccharides produced by Penicillium and Aspergillus species.
Notermans
S
S
National Institute of Public Health and Environment Hygiene, Bilthoven, The Netherlands.
Veeneman
G H
GH
van Zuylen
C W
CW
Hoogerhout
P
P
van Boom
J H
JH
D000908:Antibodies, Fungal / Q000276:immunology; D000946:Antigens, Fungal / Q000276:immunology*; D001230:Aspergillus / Q000276:immunology*; D001667:Binding, Competitive; D006801:Humans; D010407:Penicillium / Q000276:immunology*; D011134:Polysaccharides / Q000276:immunology*
D000908:Antibodies, Fungal; D000946:Antigens, Fungal; D011134:Polysaccharides