Determination of clonidine in human plasma by cold on-column injection capillary gas chromatography-selected-ion monitoring-mass spectrometry.
Clonidine [Catapres, 2-(2,6-dichlorophenylimino)-2-imidazoline hydrochloride], a potent, antihypertensive drug, given in a dosage of 0.1-0.3 mg, yields plasma levels in the ng/ml to pg/ml range. A method for the determination of clonidine from human plasma was developed, which includes a simple, one-step solvent extraction followed by a one-step derivatization with N-methyl-N-(tert.-butyldimethylsilyl)trifluoroacetamide to form a tert.-butyldimethylsilyl (TBDMS) derivative. Levels of clonidine (TBDMS) were determined by cold on-column injection capillary gas chromatography-selected-ion monitoring-mass spectrometry. Precision of the method at the 1 ng/ml level was +/- 2.4%.
Arrendale
R F
RF
Department of Medicinal Chemistry and Pharmacognosy, University of Georgia, College of Pharmacy, Athens 30602.
Stewart
J T
JT
Tackett
R L
RL
D000284:Administration, Oral; D001562:Benzimidazoles / Q000097:blood / Q000493:pharmacokinetics; D003000:Clonidine / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007275:Injections, Intravenous
D001562:Benzimidazoles; D003000:Clonidine