Complete nucleotide sequence of pAL5000, a plasmid from Mycobacterium fortuitum.
We have determined the complete nucleotide sequence of pAL5000, a plasmid from Mycobacterium fortuitum; the plasmid contains 4837 bp with 65% G + C. Five open reading frames (ORF1 to ORF5) have been identified. A number of sequences corresponding to palindromes, repeats, a helix-turn-helix motif, a signal sequence and repetitive amino acid motifs can be identified. This sequence should facilitate the construction of vectors based on pAL5000 for transfer and expression studies in mycobacteria.
Rauzier
J
J
Unité de Génie Microbiologique, Institut Pasteur, Paris, France.
Moniz-Pereira
J
J
Gicquel-Sanzey
B
B
D000595:Amino Acid Sequence; D001483:Base Sequence; D001709:Biotechnology; D003001:Cloning, Molecular; D008969:Molecular Sequence Data; D009161:Mycobacterium / Q000235:genetics*; D010957:Plasmids; D015183:Restriction Mapping