The determination of selenomethionine in selenium yeast by cyanogen bromide gas chromatography.
A new method for the determination of bonded selenomethionine in selenium yeast by gas chromatography is established. Bonded selenomethionine is rapidly and precisely determined by measuring CH3SeCN released during the reaction of selenoprotein with CNBr (CNBr-GC method). Conditions for the reaction and chromatography are described. The results of CNBr-GC are compared with those obtained by acid hydrolysis/ion exchange chromatography. This new method has the advantage of being accurate, sensitive and selective.
Zheng
O Y
OY
Department of Chemistry, Jinan University, Guangzhou, People's Republic of China.
Wu
J
J
D002849:Chromatography, Gas; D003488:Cyanogen Bromide; D007202:Indicators and Reagents; D012643:Selenium / Q000032:analysis*; D012645:Selenomethionine / Q000032:analysis*; D015003:Yeasts / Q000032:analysis*
D007202:Indicators and Reagents; D012645:Selenomethionine; D012643:Selenium; D003488:Cyanogen Bromide