Expression of human angiogenin in cultured baby hamster kidney cells.
Baby hamster kidney cells were transformed with DNA sequences derived from the gene for human angiogenin. Expression was under the transcriptional control of the inducible mouse metallothionein 1 promoter. Recombinant angiogenin was purified and shown to be chemically, biologically, and enzymatically indistinguishable from the natural product. The large-scale production of recombinant angiogenin achieved should facilitate detailed studies into the structure-function relationships of this potent angiogenic molecule.
Kurachi
K
K
Center for Biochemical and Biophysical Sciences and Medicine, Harvard Medical School, Boston, Massachusetts 02115.
Rybak
S M
SM
Fett
J W
JW
Shapiro
R
R
Strydom
D J
DJ
Olson
K A
KA
Riordan
J F
JF
Davie
E W
EW
Vallee
B L
BL
D043925:Angiogenesis Inducing Agents / Q000235:genetics*; D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D005796:Genes; D005822:Genetic Vectors; D006133:Growth Substances / Q000235:genetics*; D006801:Humans; D007668:Kidney; D009363:Neoplasm Proteins / Q000235:genetics* / Q000302:isolation & purification; D010446:Peptide Fragments / Q000032:analysis; D010957:Plasmids; D011994:Recombinant Proteins / Q000302:isolation & purification; D012259:Ribonuclease, Pancreatic; D014158:Transcription, Genetic; D014162:Transfection
D043925:Angiogenesis Inducing Agents; D006133:Growth Substances; D009363:Neoplasm Proteins; D010446:Peptide Fragments; D011994:Recombinant Proteins; C046366:angiogenin; D012259:Ribonuclease, Pancreatic