title: [The effect of potassium ferricyanide and copper (II) ions on the properties of immobilized galactose oxidase].
abstract: Preparations of galactosooxidase (EC 1.1.3.9) immobilized by activated aminorganosilica have been used to study potassium ferricyanide and bivalent copper ions on the enzyme activity and stability in continuous reactor under pulse conditions. Introduction of potassium ferricyanide is shown to activate the enzyme and inconsiderably affecting its stability with the substrate absent and inducing inactivation of galactosooxidase in the process of catalytic reaction. Cu2+ ions, exerting no effect on the activity of immobilized galactosooxidase, evoke the enzyme inactivation in the process of catalysis.
authors.forename: L V
authors.initials: LV
authors.lastname: Kondakova
authors.forename: V V
authors.initials: VV
authors.lastname: Ianishpol'skiĭ
authors.forename: V A
authors.initials: VA
authors.lastname: Tertykh
authors.forename: T T
authors.initials: TT
authors.lastname: Buglova
mesh_terms: D003300:Copper / Q000494:pharmacology*; D004789:Enzyme Activation; D004795:Enzyme Stability; D004800:Enzymes, Immobilized; D005292:Ferricyanides / Q000494:pharmacology*; D005670:Fusarium / Q000201:enzymology; D005692:Galactose Oxidase / Q000378:metabolism*; D066298:In Vitro Techniques; D007700:Kinetics; D010101:Oxygen Consumption
chemical_list: D004800:Enzymes, Immobilized; D005292:Ferricyanides; D003300:Copper; D005692:Galactose Oxidase; C028033:potassium ferricyanide
vernacular_title: Vliianie ferritsianida kaliia i ionov medi (II) na svoĭstva immobilizovannoĭ galaktozooksodazy.