Fluoride-induced changes in the tooth glycosaminoglycans: an in vivo study in the rabbit.
Effect of high fluoride ingestion on the tooth matrix glycosaminoglycans was studied in rabbits administered 10 mg NaF/kg body weight orally at 24-h intervals for a period of 9 months. Fluoride-treated tooth showed a significant reduction in glycosaminoglycan content as compared to the normal tooth. Sephadex G-75 chromatography and DEAE-cellulose ion exchange chromatography revealed the presence of small molecular weight glycosaminoglycan molecules and an increase in the charge density heterogeneity in the sulphated glycosaminoglycans of the fluoride-treated rabbit tooth as compared to the controls. These changes may be related to the dedifferentiated tooth matrix and an increase in the dermatan sulphate content in the fluoride-treated tooth matrix reported earlier.
Susheela
A K
AK
Department of Anatomy, All India Institute of Medical Sciences, New Delhi.
Sharma
K
K
D000818:Animals; D005459:Fluorides / Q000097:blood / Q000494:pharmacology*; D006025:Glycosaminoglycans / Q000378:metabolism*; D014070:Tooth / Q000187:drug effects* / Q000378:metabolism; D014574:Uronic Acids / Q000032:analysis
D006025:Glycosaminoglycans; D014574:Uronic Acids; D005459:Fluorides