title: CLUSTAL: a package for performing multiple sequence alignment on a microcomputer.
abstract: An approach for performing multiple alignments of large numbers of amino acid or nucleotide sequences is described. The method is based on first deriving a phylogenetic tree from a matrix of all pairwise sequence similarity scores, obtained using a fast pairwise alignment algorithm. Then the multiple alignment is achieved from a series of pairwise alignments of clusters of sequences, following the order of branching in the tree. The method is sufficiently fast and economical with memory to be easily implemented on a microcomputer, and yet the results obtained are comparable to those from packages requiring mainframe computer facilities.
authors.affiliation: Department of Genetics, Trinity College, Dublin, Ireland.
authors.forename: D G
authors.initials: DG
authors.lastname: Higgins
authors.forename: P M
authors.initials: PM
authors.lastname: Sharp
mesh_terms: D000465:Algorithms; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005914:Globins / Q000235:genetics; D006801:Humans; D008838:Microcomputers; D008969:Molecular Sequence Data; D010802:Phylogeny; D012341:RNA, Ribosomal, 5S / Q000235:genetics; D012984:Software
chemical_list: D012341:RNA, Ribosomal, 5S; D005914:Globins