Calcium efflux in cultured vascular smooth muscle cells from genetically hypertensive rats: effect of angiotensin II and vasopressin.
Control of cell calcium handling and transport may be abnormal in hypertension. We have studied calcium efflux rates in response to angiotensin II and vasopressin in cultured vascular smooth muscle cells from New Zealand genetically hypertensive rats and normotensive control rats. Calcium efflux with both peptides was time- and concentration-dependent and was significantly greater in cells from the genetically hypertensive rats (P less than 0.001, analysis of variance). In addition, mean +/- s.d. protein content (0.31 +/- 0.01 versus 0.28 +/- 0.02 mg/well, n = 48, P less than 0.001) and calculated total cell calcium (3.63 +/- 0.27 versus 2.86 +/- 0.32 nmol/mg protein, n = 16, P less than 0.005) were greater in genetically hypertensive rat cells. These data are consistent with the presence of abnormal cell calcium dynamics in hypertension in genetically hypertensive rats.
Harris
E L
EL
Wellcome Medical Research Institute, University of Otago Medical School, Dunedin, New Zealand.
Millar
J A
JA
D000804:Angiotensin II / Q000494:pharmacology*; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D006973:Hypertension / Q000235:genetics / Q000378:metabolism*; D009131:Muscle, Smooth, Vascular / Q000378:metabolism*; D051381:Rats; D011922:Rats, Mutant Strains; D012504:Saralasin / Q000494:pharmacology; D014667:Vasopressins / Q000494:pharmacology*
D014667:Vasopressins; D000804:Angiotensin II; D012504:Saralasin; D002118:Calcium