title: A proposal for further standardization of red blood cell glucose 6-phosphate dehydrogenase determinations.
abstract: A number of factors were identified which influence the determination of red blood cell glucose 6-phosphate dehydrogenase (G6PD). White blood cells were effectively removed from whole blood by filtration, resulting in a mean reduction of G6PD activity of greater than 1.0 U/g Hb. Optimal enzyme activity was found using haemolysates with a Hb concentration in the range 0.4-1.2 g/dl. At a constant temperature of 30 degrees C, maximum linear rate of activity was achieved between the 10th and 20th min after initiation of the reaction. As a result of these findings, modifications to standardize the assay system were introduced. The new method exhibits excellent precision (CV less than 2.0%) with normal samples, and 95% reference ranges of 7.00-11.15 U/g Hb for adult females and 7.05-10.75 U/g Hb for adult males.
authors.affiliation: Department of Haematology, Manchester Royal Infirmary.
authors.forename: J C
authors.initials: JC
authors.lastname: Ardern
authors.forename: N
authors.initials: N
authors.lastname: Edwards
authors.forename: K
authors.initials: K
authors.lastname: Hyde
authors.forename: C
authors.initials: C
authors.lastname: Jardine-Wilkinson
authors.forename: K I
authors.initials: KI
authors.lastname: Cinkotai
authors.forename: D M
authors.initials: DM
authors.lastname: Power
authors.forename: J E
authors.initials: JE
authors.lastname: MacIver
mesh_terms: D000293:Adolescent; D000328:Adult; D004909:Erythrocyte Indices; D004912:Erythrocytes / Q000032:analysis / Q000201:enzymology*; D005260:Female; D005954:Glucosephosphate Dehydrogenase / Q000097:blood*; D006452:Hemoglobinometry; D006801:Humans; D008297:Male; D008722:Methods; D008875:Middle Aged; D012015:Reference Standards
chemical_list: D005954:Glucosephosphate Dehydrogenase