Amplified gene for the epidermal growth factor receptor in a human glioblastoma cell line encodes an enzymatically inactive protein.
The gene encoding the receptor for epidermal growth factor was amplified two- to fivefold in the human glioblastoma cell line SF268. The amplified gene gave rise to abundant quantities of receptor that bound EGF with a high affinity (Kd, 0.35 nM). The binding of ligand failed to elicit cellular DNA synthesis, however, and the receptor was enzymatically inactive. We presume that the amplified receptor gene carries a mutation(s) that affects several aspects of the receptor's function. Characterization of the mutation(s) may illuminate how structure dictates function in the receptor protein.
Wells
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A
G. W. Hooper Research Foundation, University of California, San Francisco 94143.
Bishop
J M
JM
Helmeste
D
D
D002453:Cell Cycle; D004247:DNA / Q000096:biosynthesis; D004815:Epidermal Growth Factor / Q000378:metabolism; D066246:ErbB Receptors / Q000235:genetics*; D005784:Gene Amplification; D005910:Glioma / Q000235:genetics*; D006801:Humans; D009154:Mutation; D011505:Protein-Tyrosine Kinases / Q000235:genetics*; D013329:Structure-Activity Relationship; D014407:Tumor Cells, Cultured
D004815:Epidermal Growth Factor; D004247:DNA; D066246:ErbB Receptors; D011505:Protein-Tyrosine Kinases