title: Excretion of iodide in 24-h urine as determined by ion-pair reversed-phase liquid chromatography with electrochemical detection.
abstract: A simple method is presented for the routine analysis of iodide in urine. After a one-step sample clean-up, iodide was separated by ion-pair reversed-phase liquid chromatography and detected electrochemically with a silver electrode. The coefficient of variation of a single analysis of iodide in a pooled urine sample (530 nmol/l) was 7.6%. The detection limit, derived from a signal-to-noise ratio of 3, was 3 pmol, corresponding to 0.06 mumol/l. The recovery of iodide added to urine was 96 +/- 7%. The accuracy of the method was assessed by analysing ten different samples with neutron activation analysis. The data obtained with the two methods showed a high correlation (r = 0.991) and did not differ significantly. Excretion of iodide in samples of 24-h urine from a free-living population was shown to have a log-normal distribution and to be higher in men than in women. The iodide/creatinine ratio was independent of sex and increased with age.
authors.affiliation: Department of Clinical Biochemistry, TNO-CIVO Toxicology and Nutrition Institute, Zeist, The Netherlands.
authors.forename: J
authors.initials: J
authors.lastname: Odink
authors.forename: J J
authors.initials: JJ
authors.lastname: Bogaards
authors.forename: H
authors.initials: H
authors.lastname: Sandman
authors.forename: R J
authors.initials: RJ
authors.lastname: Egger
authors.forename: G A
authors.initials: GA
authors.lastname: Arkesteyn
authors.forename: P
authors.initials: P
authors.lastname: de Jong
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D004563:Electrochemistry; D005260:Female; D006801:Humans; D007202:Indicators and Reagents; D007454:Iodides / Q000652:urine*; D008297:Male; D008875:Middle Aged; D009501:Neutron Activation Analysis; D010084:Oxidation-Reduction; D012737:Sex Factors
chemical_list: D007202:Indicators and Reagents; D007454:Iodides