title: Heterogeneity in ampR-ampC gene interaction in Enterobacter cloacae.
abstract: The ampR gene and its regulation of AmpC beta-lactamase synthesis were investigated for Enterobacter cloacae 1194E, a wild-type strain producing a group A (pI 8.7) enzyme. Expression of the cloned E. cloacae 1194E ampR-ampC region was examined initially in Escherichia coli HB101. However, transformants showed only constitutive beta-lactamase expression. For study of enzyme expression in a more closely related host, the cloned E. cloacae 1194E ampR-ampC region was transformed into E. cloacae 55, a wild-type strain producing a group B (pI 7.8) enzyme. Results indicated a functional E. cloacae 1194E ampR gene that could not be transcomplemented by E. cloacae 55. A comparative analysis of ampR nucleotide and amino acid-sequence data from E. cloacae 1194E and E. cloacae MHN1 revealed related but divergent genes. Thermal induction studies of AmpC beta-lactamase also indicated a difference between E. cloacae 1194E and E. cloacae 55 in ampR-ampC interaction. Thus, it appears that, in at least some strains of Enterobacter, significant intraspecies divergence of ampR has occurred. This heterogeneity in ampR would not have been detected with beta-lactamase expression studies conducted exclusively in E. coli.
authors.affiliation: Department of Medical Microbiology, Creighton University School of Medicine, Omaha, Nebraska 68178.
authors.forename: R V
authors.initials: RV
authors.lastname: Goering
authors.forename: C C
authors.initials: CC
authors.lastname: Sanders
authors.forename: W E
authors.initials: WE
authors.lastname: Sanders
authors.forename: R
authors.initials: R
authors.lastname: Guay
authors.forename: S
authors.initials: S
authors.lastname: Guerin
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D004754:Enterobacter / Q000235:genetics*; D004755:Enterobacteriaceae / Q000235:genetics*; D005786:Gene Expression Regulation; D005798:Genes, Bacterial; D007525:Isoelectric Focusing; D008969:Molecular Sequence Data; D010957:Plasmids; D012689:Sequence Homology, Nucleic Acid; D013696:Temperature; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; C048795:AmpC beta-lactamases; D001618:beta-Lactamases