Radioiodination of interleukin 2 to high specific activities by the vapor-phase chloramine T method.
Recombinant human interleukin 2 (IL-2) was radioiodinated utilizing the vapor phase chloramine T method of iodination. The method is rapid, reproducible, and allows the efficient radioiodination of IL-2 to specific activities higher than those previously attained with full retention of biological activity. IL-2 radioiodinated by this method binds with high affinity to receptors present on phytohemagglutinin-stimulated peripheral blood lymphocytes and should be useful for the study of receptor structure and function.
Siekierka
J J
JJ
Merck, Sharp and Dohme Research Laboratories, Department of Immunology, Rahway, New Jersey 07065.
DeGudicibus
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D000818:Animals; D001665:Binding Sites; D002470:Cell Survival / Q000187:drug effects; D002700:Chloramines; D006801:Humans; D007376:Interleukin-2 / Q000032:analysis*; D007457:Iodine Radioisotopes; D007553:Isotope Labeling; D051379:Mice; D015375:Receptors, Interleukin-2 / Q000032:analysis; D013329:Structure-Activity Relationship; D014105:Tosyl Compounds; D014835:Volatilization
D002700:Chloramines; D007376:Interleukin-2; D007457:Iodine Radioisotopes; D015375:Receptors, Interleukin-2; D014105:Tosyl Compounds; C016300:chloramine-T