title: Improved transfer of high molecular weight proteins using fabric-reinforced soft polyacrylamide gels.
abstract: Rapid transfer of electrophoretically separated, high molecular weight proteins from fabric-reinforced, soft, low-concentration polyacrylamide gels (3.5%T, 2.6%C) is described. Polyester fabrics proved useful for gel reinforcement during vertical slab gel electrophoresis. The fabric-reinforced gel retains its dimension during transfer, equilibration and drying, facilitating comparison of protein bands in the separation gel and transfer matrix.
authors.affiliation: Laboratoire de Biochimie, Faculté de Médecine Chemin de Valombrose, Nice, France.
authors.forename: M
authors.initials: M
authors.lastname: Starita-Geribaldi
authors.forename: J
authors.initials: J
authors.lastname: Giudicelli
authors.forename: P
authors.initials: P
authors.lastname: Sudaka
mesh_terms: D003101:Collodion; D004591:Electrophoresis, Polyacrylamide Gel / Q000295:instrumentation; D008567:Membranes, Artificial; D008970:Molecular Weight; D011091:Polyesters; D011506:Proteins / Q000302:isolation & purification*
chemical_list: D008567:Membranes, Artificial; D011091:Polyesters; D011506:Proteins; D003101:Collodion