Isolation and characterization of a UV-sensitive mutator (mutB1) mutant of Haemophilus influenzae.
The mutB1 mutant of Haemophilus influenzae is very sensitive to UV radiation but only slightly sensitive to methylmethane sulfonate or N-methyl-N'-nitro-N-nitrosoguanidine. Cultures of mutB1 cells contain high numbers of spontaneous mutants and show hypermutability after exposure to the latter mutagen. Normally high-efficiency transforming markers, as well as low-efficiency ones, transform mutB1 recipients at similarly low efficiencies. Significant host cell reactivation was observed when mutB1 cells were exposed to UV-damaged phage; however, these mutants showed a decrease in phage recombination. This mutant did not degrade its DNA following exposure to UV. It is speculated that the mutB1 mutation is similar to the Escherichia coli uvrD mutation.
Walter
R B
RB
The University of Texas System Cancer Center, Science Park-Research Division, Smithville 78957.
Stuy
J H
JH
D004269:DNA, Bacterial / Q000032:analysis / Q000187:drug effects / Q000528:radiation effects; D006193:Haemophilus influenzae / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D008741:Methyl Methanesulfonate / Q000494:pharmacology; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology; D009154:Mutation; D014466:Ultraviolet Rays
D004269:DNA, Bacterial; D008769:Methylnitronitrosoguanidine; D008741:Methyl Methanesulfonate