title: Linear Ferguson plots of polystyrene sulfate size standards for the quantitative agarose gel electrophoresis of subcellular particles.
abstract: Accurately standardized commercial polystyrene sulfate particles in agarose gel electrophoresis yield linear Ferguson plots at pH 7.4 over a gel concentration range up to 0.9% agarose which do not exhibit any significant sigmoidal curve elements, using either a discontinuous buffer system or a continuous buffer. Ferguson plots of these standard-sized particles were evaluated using alternatively a linear or convex model, by means of a newly developed set of programs (to be used in conjunction with program M-LAB) which (i) is sufficiently user-friendly to allow for quantitative agarose gel electrophoresis of subcellular-sized spherical particles based on their convex Ferguson plots with the same operational simplicity previously available for linear Ferguson plots only; (ii) simultaneously and interactively analyzes the Ferguson plots of all particles under consideration on the basis of an extended Ogston model.
authors.affiliation: Section on Macromolecular Analysis, National Institute of Child Health and Human Development, Bethesda, MD 20892.
authors.forename: E
authors.initials: E
authors.lastname: Hahn
authors.forename: L
authors.initials: L
authors.lastname: Wurts
authors.forename: D
authors.initials: D
authors.lastname: Tietz
authors.forename: A
authors.initials: A
authors.lastname: Chrambach
mesh_terms: D001330:Electronic Data Processing; D004587:Electrophoresis, Agar Gel; D008433:Mathematics; D010316:Particle Size; D011137:Polystyrenes / Q000032:analysis*; D012015:Reference Standards; D013347:Subcellular Fractions
chemical_list: D011137:Polystyrenes