Valinomycin-induced chloride permeability in isolated rat liver mitochondria.
1. Ionophore-induced osmotic swelling was used to study Cl- transport in isolated rat liver mitochondria. 2. Energy-dependent, neutral ionophore-induced swelling in Cl- salts at pH 7.2 required K+ and was preceded by a brief lag phase that was absent in chlorotributyltin-induced swelling. 3. Treatments that stimulated or inhibited mitochondrial K+/H+ exchange had qualitatively similar effects on both valinomycin-induced swelling and the associated lag phase. 4. The results suggest that valinomycin-induced Cl- permeability results from an interaction between the K+/H+ antiporter and neutral ionophore K+ complexes.
Schnell
F C
FC
Crop Science Department, North Carolina State University, Raleigh 27695-7620.
Moreland
D E
DE
D000818:Animals; D002712:Chlorides / Q000493:pharmacokinetics*; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008027:Light; D008274:Magnesium / Q000378:metabolism; D008297:Male; D008930:Mitochondria, Liver / Q000187:drug effects* / Q000378:metabolism; D009994:Osmolar Concentration; D009997:Osmotic Pressure; D010101:Oxygen Consumption / Q000187:drug effects; D010539:Permeability; D051381:Rats; D011919:Rats, Inbred Strains; D012542:Scattering, Radiation; D014634:Valinomycin / Q000494:pharmacology*
D002712:Chlorides; D014634:Valinomycin; D008274:Magnesium