title: The role of protein kinase C activation in signal transmission by interleukin 2.
abstract: Role of protein kinase C (PKC) in interleukin (IL) 2-induced proliferation was investigated by utilizing two murine IL 2-dependent cell lines, CT6 and CTLL-2 cell lines. CT6 cells showed a marked proliferative response to phorbol 12-myristate 13-acetate (PMA), while CTLL-2 did not. PMA induced PKC translocation from cytosol to membrane only in a PMA-responsive cell line. IL 2 failed to stimulate PKC translocation in both cell lines. H-7, a potent and specific PKC inhibitor, however, inhibited the proliferation of both cell lines induced by IL 2. Taken collectively, IL 2 may induce PKC activation without its translocation.
authors.affiliation: Department of Clinical Pathology, Jichi Medical School, Tochigi-ken, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Mukaida
authors.forename: H
authors.initials: H
authors.lastname: Yagisawa
authors.forename: T
authors.initials: T
authors.lastname: Kawai
authors.forename: T
authors.initials: T
authors.lastname: Kasahara
mesh_terms: D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002462:Cell Membrane / Q000201:enzymology; D003600:Cytosol / Q000201:enzymology; D007376:Interleukin-2 / Q000494:pharmacology*; D007700:Kinetics; D051379:Mice; D011493:Protein Kinase C / Q000378:metabolism*; D011994:Recombinant Proteins / Q000494:pharmacology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D007376:Interleukin-2; D011994:Recombinant Proteins; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate