Determination of molecular weight of native proteins by polyacrylamide gradient gel electrophoresis.
An improved method for the estimation of molecular weights of native proteins by polyacrylamide gel electrophoresis, in 9 cm x 9 cm x 0.05 mm 4-20% T fabric reinforced gradient gels, is described. Plotting the logarithm of the relative mobilities of proteins versus gel concentrations produces lines whose slopes are related to molecular weights.
Nishizawa
H
H
Kyoritsu College of Pharmacy, Tokyo, Japan.
Kita
N
N
Okimura
S
S
Takao
E
E
Abe
Y
Y
D002138:Calibration; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D006801:Humans; D008970:Molecular Weight; D011506:Proteins / Q000032:analysis*; D014325:Tromethamine
D011506:Proteins; D014325:Tromethamine