[Interaction of blood lymphocyte Fc receptors with IgG during in vitro cultivation].
Bovine blood lymphocytes taken from normal cows and those suffering from chronic lymphocytic leukemia were cultured in complete medium 199 with 10% of heat-inactivated fetal bovine serum. After a 48 hour culturing an enhanced quantity of the Fc-receptor bound fluoresceinated immunoglobulin G (IgG) was established. When lymphocyte fractions enriched with T- or B-cells were cultured, the binding capacity of Fc-receptors for IgG (48 hours after culture) increased in both the cell populations. A study of the kinetics of interactions of Fc-receptors with IgG showed that the increased number of Fc-receptors after culturing was followed by an enhanced affinity of Fc-receptors towards IgG. The affinity of Fc-receptors of blood lymphocytes of cows with chronic lymphocytic leukemia was lower than that of normal lymphocytes.
Dikinene
N P
NP
Miliukene
V V
VV
D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism*; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood; D002478:Cells, Cultured; D005260:Female; D007074:Immunoglobulin G / Q000378:metabolism*; D007700:Kinetics; D007945:Leukemia, Lymphoid / Q000097:blood / Q000662:veterinary; D011961:Receptors, Fc / Q000378:metabolism*; D013601:T-Lymphocytes / Q000378:metabolism*; D013997:Time Factors; D014407:Tumor Cells, Cultured
D007074:Immunoglobulin G; D011961:Receptors, Fc
Vzaimodeĭstvie Fc-retseptorov limfotsitov krovi s IgG pri kul'tivirovanii in vitro.