Two-dimensional electrophoresis with immobilized pH gradients of leaf proteins from barley (Hordeum vulgare): method, reproducibility and genetic aspects.
Leaf proteins from 14 barley cultivars (Hordeum vulgare) were analyzed by two-dimensional electrophoresis with immobilized pH gradients (IPG 4-7 and IPG 6-10) in the first dimension. Highly reproducible two-dimensional patterns were obtained, owing to constant spot positions along the isoelectric focusing axis. A number of variety-specific protein spots were detected, allowing us to discriminate barley cultivars not only into main groups but into individual cultivars.
Görg
A
A
Lehrstuhl für Allgemeine, Lebensmitteltechnologie, Technische Universität München, Freising-Weihenstephan, Federal Republic of Germany.
Postel
W
W
Domscheit
A
A
Günther
S
S
D002523:Edible Grain / Q000032:analysis*; D015180:Electrophoresis, Gel, Two-Dimensional; D014644:Genetic Variation; D001467:Hordeum / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D010449:Peptide Mapping; D010940:Plant Proteins / Q000032:analysis* / Q000235:genetics
D010940:Plant Proteins