Lack of vimentin occurring during the intrafollicular stages of B cell development characterizes follicular center cell lymphomas.
Reactive germinal-center B cells completely lack vimentin. This observation made by using monoclonal vimentin antibody V9 and a sensitive immunoperoxidase technique led to the investigation of vimentin expression in an unselected series of 73 immunohistochemically proven B cell lymphomas. While small lymphocytic lymphomas regularly expressed vimentin, the neoplastic population of mixed, small and large, and large cell lymphomas often contained various proportions of vimentin-negative tumor cells or failed to express it at all. Against the background of the Lukes-Collins classification and the Kiel classification, both defining and subdividing follicular center-cell lymphomas, there were statistically highly significant correlations between the absence of vimentin and the histology, suggesting a germinal center origin. Our data suggest that loss of vimentin expression is indicative of the transient, follicular center-cell stage of normal and malignant B lymphocytic transformation. The complete absence of vimentin within the neoplastic population of all Burkitt's lymphoma cases examined may serve as an argument for its follicular center stage of differentiation, as was asserted by Lukes and Collins.
Möller
P
P
Institute of Pathology, University of Heidelberg, Federal Republic of Germany.
Momburg
F
F
Hofmann
W J
WJ
Matthaei-Maurer
D U
DU
D001402:B-Lymphocytes / Q000032:analysis / Q000378:metabolism* / Q000473:pathology; D002454:Cell Differentiation; D002471:Cell Transformation, Neoplastic / Q000378:metabolism / Q000473:pathology; D006801:Humans; D008198:Lymph Nodes / Q000378:metabolism / Q000473:pathology* / Q000502:physiology; D008213:Lymphocyte Activation; D008223:Lymphoma / Q000145:classification / Q000378:metabolism* / Q000473:pathology; D010641:Phenotype; D014746:Vimentin / Q000032:analysis / Q000172:deficiency*
D014746:Vimentin