Vasodilatation with pinacidil. Mode of action in rat resistance vessels.
Pinacidil is a newly developed antihypertensive vasodilator, proposed to belong to the new group of smooth muscle relaxants, the K+ channel openers. The in vitro effects of pinacidil on induced tone, smooth muscle membrane potential and 86Rb and 42K efflux from rat resistance vessels (internal diameter about 200 microns) were studied. Tone induced with noradrenaline was concentration-dependently inhibited by pinacidil. Responses to electrical field stimulation were also inhibited. However, tone induced with high K+ depolarisation, noradrenaline in the presence of high K+, caffeine-induced contractions and noradrenaline contractions in the presence of felodipine were little affected by pinacidil. Pinacidil caused concentration-dependent hyperpolarisation of the resting smooth muscle. Pinacidil caused only a small and transient increase of the 86Rb efflux rate constant, while the same concentrations of pinacidil produced a significant increase in the 42K efflux rate constant. Our results seem to indicate that the relaxant effect of pinacidil is the result of an increase in K+ permeability, thus causing hyperpolarisation and relaxation. The opened K+ channels appear to be selective for K+ over Rb+.
Videbaek
L M
LM
Institute of Biophysics, University of Aarhus, Denmark.
Aalkjaer
C
C
Mulvany
M J
MJ
D000818:Animals; D001808:Blood Vessels / Q000187:drug effects / Q000502:physiology*; D015736:Felodipine; D006146:Guanidines / Q000494:pharmacology*; D066298:In Vitro Techniques; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D009121:Muscle Denervation; D009131:Muscle, Smooth, Vascular / Q000187:drug effects*; D009568:Nitrendipine / Q000031:analogs & derivatives / Q000494:pharmacology; D020110:Pinacidil; D011188:Potassium / Q000494:pharmacology; D011197:Potassium Radioisotopes; D051381:Rats; D011921:Rats, Inbred WKY; D012413:Rubidium / Q000378:metabolism; D012414:Rubidium Radioisotopes; D013152:Splanchnic Circulation / Q000187:drug effects; D014655:Vascular Resistance / Q000187:drug effects; D014665:Vasodilator Agents / Q000494:pharmacology*
D006146:Guanidines; D011197:Potassium Radioisotopes; D012414:Rubidium Radioisotopes; D014665:Vasodilator Agents; D020110:Pinacidil; D009568:Nitrendipine; D012413:Rubidium; D015736:Felodipine; D011188:Potassium