Galvanotaxis of human granulocytes. Dose-response curve.
The galvanotactic response of human granulocytes was investigated theoretically and experimentally. The basic results are: (i) The granulocytes move towards the anode. (ii) The directed movement has been quantified by two different polar order parameters--the McCutcheon index and the average of cos phi. (iii) The polar order parameters are a function of the applied electric field (= dose-response curve). (iv) The inverse of the galvanotactic constant of migrating cells (analogous to the Michaelis-Menten constant) has a value of -0.2 +/- 0.03 V/mm. (v) The galvanotactic response of granulocytes is a non-cooperative process with a cooperativity coefficient of 1 +/- 0.2. (vi) The galvanotactic constant is a function of pH. (vii) The protein essential for the galvanotactic response is very likely a G-protein.
Rapp
B
B
Abteilung für Biophysik, Universität Ulm, Federal Republic of Germany.
de Boisfleury-Chevance
A
A
Gruler
H
H
D002465:Cell Movement; D004560:Electricity; D006098:Granulocytes / Q000502:physiology*; D006801:Humans; D066298:In Vitro Techniques