title: A human urine-derived interleukin 1 inhibitor. Homology with deoxyribonuclease I.
abstract: We have previously reported that the urine of febrile humans contained large quantities of an inhibitor of IL-1-induced murine thymocyte proliferation that was a glycoprotein between 30 and 40 kD in size. In the present study this factor has been purified to homogeneity using a sequence of eight purification steps (ammonium sulfate precipitation, ion exchange chromatography, molecular sieve chromatography, hydrophobic affinity chromatography, hydroxylapatite chromatography, fast protein liquid chromatography, and two HPLC steps). SDS-PAGE analysis indicates that the purified material is a 38-kD molecule. Evidence based on a partial amino acid sequence analysis as well as enzyme studies indicates that this inhibitor is a type of human DNase I.
authors.affiliation: Department of Medicine, Albert Einstein College of Medicine, Bronx, New York 10461.
authors.forename: D L
authors.initials: DL
authors.lastname: Rosenstreich
authors.forename: J H
authors.initials: JH
authors.lastname: Tu
authors.forename: P R
authors.initials: PR
authors.lastname: Kinkade
authors.forename: I
authors.initials: I
authors.lastname: Maurer-Fogy
authors.forename: J
authors.initials: J
authors.lastname: Kahn
authors.forename: R W
authors.initials: RW
authors.lastname: Barton
authors.forename: P R
authors.initials: PR
authors.lastname: Farina
mesh_terms: D000595:Amino Acid Sequence; D003850:Deoxyribonuclease I / Q000378:metabolism; D006023:Glycoproteins / Q000652:urine*; D006801:Humans; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1 / Q000037:antagonists & inhibitors*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011506:Proteins / Q000302:isolation & purification*; D012689:Sequence Homology, Nucleic Acid; D012795:Sialoglycoproteins
chemical_list: D006023:Glycoproteins; C507763:IL1RN protein, human; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1; D011506:Proteins; D012795:Sialoglycoproteins; D003850:Deoxyribonuclease I