title: Preclinical assessment of 90Y-labeled monoclonal antibody CO17-1A, a potential agent for radioimmunotherapy of colorectal carcinoma.
abstract: Monoclonal antibody CO17-1A was radiolabeled with 90Y using the cyclic DTPA anhydride technique and administered intravenously to athymic nude mice bearing SW 948 human colorectal carcinomas. The tumor specificity of 90Y-CO17-1A was improved by coadministration of 100 micrograms of the unlabeled antibody per animal and by purification using HPLC instead of column gel filtration chromatography. Absorbed radiation dose estimates for 90Y-CO17-1A were calculated. The radiation dose to the bone marrow will limit the amount of 90Y-CO17-1A that can be administered for cancer therapy.
authors.affiliation: Medical and Health Sciences Division, Oak Ridge Associated Universities, TN 37831.
authors.forename: L C
authors.initials: LC
authors.lastname: Washburn
authors.forename: Y C
authors.initials: YC
authors.lastname: Lee
authors.forename: T T
authors.initials: TT
authors.lastname: Sun
authors.forename: B
authors.initials: B
authors.lastname: Byrd
authors.forename: J E
authors.initials: JE
authors.lastname: Crook
authors.forename: M G
authors.initials: MG
authors.lastname: Stabin
authors.forename: Z
authors.initials: Z
authors.lastname: Steplewski
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000627:therapeutic use*; D015179:Colorectal Neoplasms / Q000532:radiotherapy*; D005260:Female; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D014018:Tissue Distribution; D014183:Transplantation, Heterologous; D015021:Yttrium Radioisotopes / Q000627:therapeutic use*
chemical_list: D000911:Antibodies, Monoclonal; D015021:Yttrium Radioisotopes