HLA-E. A novel HLA class I gene expressed in resting T lymphocytes.
A MHC class I gene, HLA-6.2, has been identified. Examination of its primary structure indicates that HLA-6.2 is the most divergent member of the class I gene family characterized to date. Tissue distribution of HLA-6.2 RNA differs from other class I genes in that the highest level of RNA is detected in resting T lymphocytes. After transfer of the HLA-6.2 gene into a class I null human lymphoblastoid cell line, a protein associated with beta 2-microglobulin is detected. Analysis of HLA deletion mutant cell lines places the HLA-6.2 gene between the HLA-C and HLA-A loci on the short arm of chromosome 6. These data support the fact that the HLA-6.2 gene is a distinct expressible HLA class I gene, which we propose be designated HLA-E.
Koller
B H
BH
Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis.
Geraghty
D E
DE
Shimizu
Y
Y
DeMars
R
R
Orr
H T
HT
D000595:Amino Acid Sequence; D001483:Base Sequence; D002460:Cell Line; D002874:Chromosome Mapping; D002896:Chromosomes, Human, Pair 6; D003001:Cloning, Molecular; D005796:Genes; D005805:Genes, MHC Class I; D006680:HLA Antigens / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D015395:Histocompatibility Antigens Class I; D006801:Humans; D007399:Interphase; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000378:metabolism; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D014162:Transfection; D000097625:HLA-E Antigens
D006680:HLA Antigens; D015395:Histocompatibility Antigens Class I; D012333:RNA, Messenger; D000097625:HLA-E Antigens