Automated sequential trace enrichment of dialysates and robotics. A technique for the preparation of biological samples prior to high-performance liquid chromatography.
The development of the sample preparation process, the automated sequential trace enrichment of dialysates, in association with a cartesian robotic sampler is described. The system has been applied to the total automation of the preparation of biological samples and high-performance liquid chromatographic analysis. Concepts of the technique are reported together with an examination of its application to free and total analyte estimation. Examples of chromatographic separations obtained from the preparation of a variety of different analytes and sample materials are given.
Cooper
J D
JD
Department of Biochemistry, Coventry and Warwickshire Hospital, U.K.
Turnell
D C
DC
Green
B
B
Verillon
F
F
D000596:Amino Acids / Q000032:analysis; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation*; D003956:Dialysis; D005938:Glucocorticoids / Q000097:blood; D006801:Humans; D006863:Hydrogen-Ion Concentration; D012371:Robotics; D013806:Theophylline / Q000378:metabolism
D000596:Amino Acids; D005938:Glucocorticoids; D013806:Theophylline