title: Reliability of biotinylated DNA probes in colony hybridization: evaluation of an improved colony lysis method for detection of TEM-1 beta-lactamase.
abstract: Utilizing an improved method for colony hybridization developed by Haas & Fleming, biotin and 32P-labelled TEM-1 probes were compared for sensitivity and specificity in identifying the type of beta-lactamase made by over 100 clinical bacterial isolates. The new procedure was more reliable than a standard one, but still gave more than 20% false positive and false negative reactions.
authors.affiliation: Massachusetts General Hospital, Boston 02114.
authors.forename: S
authors.initials: S
authors.lastname: Huovinen
authors.forename: P
authors.initials: P
authors.lastname: Huovinen
authors.forename: G A
authors.initials: GA
authors.lastname: Jacoby
mesh_terms: D001419:Bacteria / Q000145:classification / Q000201:enzymology*; D001426:Bacterial Proteins / Q000032:analysis* / Q000235:genetics; D001710:Biotin; D004274:DNA, Recombinant; D004352:Drug Resistance, Microbial; D007525:Isoelectric Focusing; D009693:Nucleic Acid Hybridization; D011237:Predictive Value of Tests; D001618:beta-Lactamases / Q000032:analysis* / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D004274:DNA, Recombinant; D001710:Biotin; D001618:beta-Lactamases; C034413:beta-lactamase TEM-1