Purification of monoclonal antibodies against the low-density lipoprotein receptor by preparative isotachophoresis.
A preparative free-flow isotachophoretic method for the purification of monoclonal antibodies from mouse ascites fluid and tissue culture media is described. This high-resolution method allows the direct separation of monoclonal antibodies from antibody-containing tissue culture media or ascites fluid and gives a better separation from the major contaminant protein fractions and a higher recovery of the monoclonal antibodies than anion-exchange chromatography. The purification can run continuously and without any time-consuming regeneration procedures; the monoclonal antibody is obtained under mild conditions in a small electrolyte volume.
Schmitz
G
G
Institut für Klinische Chemie, Westfälischen Wilhelms-Universität, Münster, F.R.G.
Böttcher
A
A
Kahl
H G
HG
Brüning
T
T
D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D002499:Centrifugation, Density Gradient; D002848:Chromatography, DEAE-Cellulose; D002852:Chromatography, Ion Exchange; D046508:Culture Techniques; D004586:Electrophoresis; D004591:Electrophoresis, Polyacrylamide Gel; D005434:Flow Cytometry; D006825:Hybridomas / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D011973:Receptors, LDL / Q000276:immunology*; D013205:Staphylococcal Protein A / Q000302:isolation & purification
D000911:Antibodies, Monoclonal; D011973:Receptors, LDL; D013205:Staphylococcal Protein A