Expression of adrenocorticotropin-releasing hormone precursor gene in placenta and other nonhypothalamic tissues in man.
Adrenocorticotropin-releasing hormone (CRH) is a peptide originally isolated from the hypothalamus. Immunocytochemical and RIA studies have revealed that CRH-like peptide is also localized in human nonhypothalamic tissues and some tumors. To see if CRH is synthesized in these nonhypothalamic tissues and tumors, we examined preproCRH mRNA in these tissues by Northern blot analysis using a cloned human preproCRH gene as a probe. PreproCRH mRNA was detected in human hypothalamus, cerebral cortex, adrenal gland, placenta, pheochromocytoma, and thymic carcinoid. The content of preproCRH mRNA in placenta was apparently greater than that in the whole hypothalamus.
Usui
T
T
Department of Medicine, Kyoto University Faculty of Medicine, Japan.
Nakai
Y
Y
Tsukada
T
T
Jingami
H
H
Takahashi
H
H
Fukata
J
J
Imura
H
H
D003001:Cloning, Molecular; D003346:Corticotropin-Releasing Hormone / Q000235:genetics*; D005786:Gene Expression Regulation; D006801:Humans; D007150:Immunohistochemistry; D009693:Nucleic Acid Hybridization; D010920:Placenta / Q000378:metabolism*; D011498:Protein Precursors / Q000235:genetics; D012333:RNA, Messenger; D011863:Radioimmunoassay; D012016:Reference Values
D011498:Protein Precursors; D012333:RNA, Messenger; C041645:pro-corticotropin releasing hormone; D003346:Corticotropin-Releasing Hormone