title: Isolation, characterization, and molecular cloning of cryptic plasmids isolated from Edwardsiella ictaluri.
abstract: Fifty-five isolates of Edwardsiella ictaluri were examined for the presence of plasmid DNA by a rapid alkaline extraction procedure. All 49 isolates from channel catfish and a single isolate from Bengal danio carried 2 plasmids with molecular masses of approximately 3.2 and 3.7 megadaltons (Mdal). Five E ictaluri isolates from other fish contained 1 to 3 plasmids, which had molecular masses ranging from 2.5 to 45 Mdal. The 2 plasmids (3.2 and 3.7 Mdal) from the type strain of E ictaluri (ATCC 33202) were ligated into pUC19 cloning vectors, and restriction endonuclease maps of each insert were prepared.
authors.affiliation: Department of Pathobiology, College of Veterinary Medicine, Auburn University, AL 36849-3501.
authors.forename: J C
authors.initials: JC
authors.lastname: Newton
authors.forename: R C
authors.initials: RC
authors.lastname: Bird
authors.forename: W T
authors.initials: WT
authors.lastname: Blevins
authors.forename: G R
authors.initials: GR
authors.lastname: Wilt
authors.forename: L G
authors.initials: LG
authors.lastname: Wolfe
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D004755:Enterobacteriaceae / Q000235:genetics* / Q000472:pathogenicity; D005399:Fishes; D007059:Ictaluridae; D010957:Plasmids; D015183:Restriction Mapping; D014774:Virulence
chemical_list: D004269:DNA, Bacterial