title: The role of arterial endothelial cell mitosis in macromolecular permeability.
abstract: The present experiments were performed on twelve male Wistar rats to study the quantitative, topographic correlation between transendothelial permeability of Evans Blue-albumin (EBA) conjugate and endothelial cell replication at the single-cell level. En face preparations of the thoracic aorta were examined by fluorescence microscopy. We found a high degree of correlation between endothelial cell mitosis and EBA leaky spots. Although endothelial cell mitosis is very rare in occurrence, nearly all junctions around the dividing cells were leaky (99%), in contrast to only 0.03% of the non-mitotic cells. In addition, electron microscopic observations showed that the junction around a dividing endothelial cell is leaky, whereas that around a dying cell is not. With the aid of our theoretical model, we were able to analyze the dynamics of macromolecular passage through leaky endothelial junctions. The duration of endothelial cell mitosis was estimated to be 67 min, which constituted 0.01% of the duration of the total cell cycle. The time-dependent change in junctional geometry during endothelial cell turnover leads to an inverse relationship between macromolecular size and duration of junctional leakage. For albumin the duration of leakiness across aortic endothelial cell is approximately 3.7 hr. The present findings lend support to our hypothesis that transiently open junctions surrounding the dividing endothelial cells provide the major pathway through which macromolecules enter the subendothelial space to result in lipid accumulation.
authors.affiliation: Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan, ROC.
authors.forename: S
authors.initials: S
authors.lastname: Chien
authors.forename: S J
authors.initials: SJ
authors.lastname: Lin
authors.forename: S
authors.initials: S
authors.lastname: Weinbaum
authors.forename: M M
authors.initials: MM
authors.lastname: Lee
authors.forename: K M
authors.initials: KM
authors.lastname: Jan
mesh_terms: D000818:Animals; D001013:Aorta, Thoracic / Q000166:cytology* / Q000378:metabolism; D001692:Biological Transport; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D005070:Evans Blue; D008297:Male; D008856:Microscopy, Fluorescence; D008938:Mitosis; D008955:Models, Cardiovascular; D010539:Permeability; D051381:Rats; D011919:Rats, Inbred Strains; D012710:Serum Albumin, Bovine / Q000378:metabolism*
chemical_list: D012710:Serum Albumin, Bovine; D005070:Evans Blue