A simple radioimmunoassay of human interleukin-2.
We have developed a liquid phase radioimmunoassay of human interleukin-2 which uses inexpensive commercially available reagents exclusively. The assay is simple, reproducible and specific in detecting different batches of human interleukin-2 of natural as well as recombinant origin, but not detecting recombinant murine interleukin-2. The assay is sensitive to a concentration of approximately 0.05 ng/ml and can be used in measurement of IL-2 in serum containing culture media.
Lee
T P
TP
Department of Medicine, Veterans Administration Medical Center, Buffalo, NY 14215.
Mookerjee
B K
BK
Pauly
J L
JL
D000906:Antibodies / Q000276:immunology; D000918:Antibody Specificity; D003429:Cross Reactions; D006801:Humans; D007376:Interleukin-2 / Q000032:analysis* / Q000096:biosynthesis / Q000276:immunology; D007700:Kinetics; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000378:metabolism; D011863:Radioimmunoassay; D011994:Recombinant Proteins / Q000032:analysis / Q000276:immunology
D000906:Antibodies; D007376:Interleukin-2; D011994:Recombinant Proteins