[Controlled ligation of oligodeoxyribonucleotides of DNA-ligase of the T4 phage].
The enzymatic ligation of 5-10-membered synthetic oligodeoxyribonucleotides forming the complementary DNA-like duplexes has been studied. The possibility of selective DNA ligase catalyzed ligation of 5'-adenylylated derivatives of oligonucleotides in the absence of rATP and also the selective joining of cohesive ends in the presence of blunt ends in the complex mixtures of oligonucleotides at definite concentrations of rATP have been demonstrated. The influence of length and sequence of short synthetic DNA-duplexes on the efficiency of ligation has been shown. We have identified the unusual octanucleotide dpTATAATAT, that being able to form concatemer complexes could not be polymerized by T4 DNA ligase.
Koroleva
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ON
Drutsa
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VL
D001345:Autoradiography; D001483:Base Sequence; D002384:Catalysis; D004247:DNA; D011088:DNA Ligases / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D011118:Polynucleotide Ligases / Q000378:metabolism*; D013604:T-Phages / Q000201:enzymology*
D009838:Oligodeoxyribonucleotides; D004247:DNA; D011088:DNA Ligases; D011118:Polynucleotide Ligases
Kontroliruemoe soedinenie oligodezoksiribonukleotidov DNA-ligazoĭ faga T4.