title: Detection of tetrodotoxin by thin-layer chromatography/fast atom bombardment mass spectrometry.
abstract: A new method for detection of tetrodotoxin (TTX) by thin-layer chromatography/fast atom bombardment (FAB) mass spectrometry was developed. TTX and/or related substances were separated by TLC on LHP-K high-performance precoated plates, with a solvent system of pyridine:ethyl acetate:acetic acid:water (15:5:3:4). The plates were subjected to positive FAB mass spectrometry, under scanning within a mass range from m/z 100 to 500. TTX was identified by selected ion-monitored chromatograms at m/z 320 (M + H)+ and 302 (M + H - H2O)+, along with full scan positive ion FAB mass spectrometry. The limit of detection for TTX was about 0.1 micrograms. TTX was also detected by cellulose acetate membrane electrophoresis/FAB mass spectrometry.
authors.affiliation: Department of Food Science and Technology, Tokyo University of Fisheries, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Nagashima
authors.forename: S
authors.initials: S
authors.lastname: Nishio
authors.forename: T
authors.initials: T
authors.lastname: Noguchi
authors.forename: O
authors.initials: O
authors.lastname: Arakawa
authors.forename: S
authors.initials: S
authors.lastname: Kanoh
authors.forename: K
authors.initials: K
authors.lastname: Hashimoto
mesh_terms: D000818:Animals; D003386:Brachyura / Q000032:analysis; D002855:Chromatography, Thin Layer / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D011799:Quinazolines / Q000032:analysis; D013779:Tetrodotoxin / Q000031:analogs & derivatives / Q000032:analysis*
chemical_list: D011799:Quinazolines; C047739:anhydrotetrodotoxin; C059207:tetrodonic acid; D013779:Tetrodotoxin