Analysis of B. malayi microfilarial antigens by immunoblotting.
BALB/C mice were immunized with heavy or low infection of live B. malayi microfilariae or immunised with different fractions of the microfilarial antigens. Antibody levels and antibody dependent macrophage mediated cytotoxicity to B. malayi microfilariae were determined in the immunized sera. Antigens responsible for induction of antibodies were recognised in B. malayi microfilarial extract by immunoblotting. Appearance of cytotoxic antibodies correlates with recognition of certain common antigens in microfilarial extract such as 45, 54, 62, 66 and 76 KDa mol. wt. proteins.
Parab
P B
PB
Pharma Division, Research Centre, HINDUSTAN CIBA-GEIGY Ltd., Bombay, India.
Rajasekariah
G R
GR
Carvalho
P A
PA
Subrahmanyam
D
D
D000818:Animals; D000909:Antibodies, Helminth / Q000032:analysis; D000917:Antibody Formation; D000920:Antibody-Dependent Cell Cytotoxicity; D000947:Antigens, Helminth / Q000032:analysis*; D002009:Brugia / Q000276:immunology*; D007114:Immunization; D015151:Immunoblotting; D051379:Mice; D008807:Mice, Inbred BALB C; D008842:Microfilariae / Q000276:immunology
D000909:Antibodies, Helminth; D000947:Antigens, Helminth