A novel method for labelling of leukocytes with technetium-99m and its comparative evaluation for abscess scintigraphy.
A new method, based on the pretreatment of leukocytes with glucoheptonate prior to treating with reduced 99mTc, has been developed for the preparation of 99mTc labelled leukocytes. The leukocytes labelled with a 99mTc concentration (5.59%/g tissue) similar to that of 111In-leukocytes (6.27%/g tissue), in the experimental abscess were in rat thigh. Concentration of 99mTc-leukocytes in blood at 24 h was only about 35% as compared to that of 111In-leukocytes. Biodistribution in the rat organs was similar in both cases, except in the liver where 99mTc-leukocytes exhibited about 4-fold greater concentration. Images of experimental abscess in rat by using 99mTc-leukocytes were comparable to those obtained with 111In-leukocytes.
Babbar
A
A
Institute of Nuclear Medicine & Allied Sciences, Delhi, India.
Mishra
P
P
Chauhan
U P
UP
D000038:Abscess / Q000000981:diagnostic imaging*; D000818:Animals; D007205:Indium Radioisotopes; D007553:Isotope Labeling / Q000379:methods*; D007962:Leukocytes / Q000187:drug effects; D011877:Radionuclide Imaging; D051381:Rats; D013400:Sugar Acids / Q000494:pharmacology; D013667:Technetium
D007205:Indium Radioisotopes; D013400:Sugar Acids; D013667:Technetium; C007852:alpha-glucoheptonic acid