Fracture healing and osteocalcin metabolism in vitamin K deficiency.
Osteocalcin (a vitamin K-dependent, bone-specific protein) is widely accepted as a marker of osteoblastic activity. The present study was conducted to determine if a vitamin K deficiency would affect fracture healing by virtue of an alteration in osteocalcin metabolism. Thirty male Sprague-Dawley rats were divided into two groups. The control group was fed a diet that was lacking in, but offered water replete with vitamin K. The experimental group was fed a vitamin K-deficient diet and was offered water that was lacking in vitamin K. After two weeks, vitamin K deficiency was established in the experimental group as shown by decreased urinary excretion of gamma-carboxyglutamic acid and an elevation of serum prothrombin times to between two to two and one-half times the control values. At this time, a standard, closed femoral fracture was produced. Six weeks later, the animals were killed. The bones were biomechanically tested in torsion. Subsequent to mechanical testing, the calluses were retrieved, and the osteocalcin content and the degree of gamma carboxylation of the osteocalcin in the calluses were measured. The results show that despite significant alterations in the gamma carboxylation of osteocalcin and elevation of prothrombin times to two to two and one-half times the control values, there were no differences in the mechanical properties of the calluses. Furthermore, there were no differences in the content or gamma carboxylation of osteocalcin in these calluses. Apparently, in vitamin K deficiency, fracture callus achieves normal mechanical properties and may have a mechanism for the gamma carboxylation of glutamic acids in osteocalcin despite a substantial depression of this activity in the rest of the body.
Einhorn
T A
TA
Musculoskeletal Research Laboratory, Mount Sinai School of Medicine, New York, New York 10029.
Gundberg
C M
CM
Devlin
V J
VJ
Warman
J
J
D015055:1-Carboxyglutamic Acid / Q000032:analysis*; D000818:Animals; D002146:Bony Callus / Q000032:analysis; D002135:Calcium-Binding Proteins / Q000032:analysis*; D005264:Femoral Fractures / Q000503:physiopathology*; D008297:Male; D015675:Osteocalcin; D011517:Prothrombin Time; D051381:Rats; D011919:Rats, Inbred Strains; D014812:Vitamin K / Q000378:metabolism; D014813:Vitamin K Deficiency / Q000503:physiopathology*; D014945:Wound Healing
D002135:Calcium-Binding Proteins; D015675:Osteocalcin; D014812:Vitamin K; D015055:1-Carboxyglutamic Acid