Competitive direct enzyme-linked immunosorbent assay for detection of sulfamethazine residues in swine urine and muscle tissue.
A sensitive assay for the detection of sulfamethazine in swine urine and muscle tissue using a direct competitive enzyme-linked immunosorbent assay (ELISA) has been developed. Undiluted urine or a phosphate-buffered saline extract of pork muscle tissue is mixed with an enzyme-labeled conjugate of sulfamethazine and horseradish peroxidase. The mixture is added to wells of a microtiter plate coated with antibody to sulfamethazine. After the test system is incubated, washed, and re-incubated with substrate and the reaction is stopped, the absorbance is measured at 405 nm. Levels of sulfamethazine as low as 20 ng sulfamethazine/g muscle tissue and 10 ng sulfamethazine/mL swine urine were detected and estimated.
Dixon-Holland
D E
DE
Rutgers University, Department of Biochemistry and Microbiology, New Brunswick, NJ 08903.
Katz
S E
SE
D000818:Animals; D004350:Drug Residues / Q000032:analysis* / Q000652:urine; D004797:Enzyme-Linked Immunosorbent Assay; D007202:Indicators and Reagents; D009132:Muscles / Q000032:analysis*; D011817:Rabbits; D013418:Sulfamethazine / Q000032:analysis* / Q000652:urine; D013552:Swine
D007202:Indicators and Reagents; D013418:Sulfamethazine