Treatment of experimental myasthenia gravis with total lymphoid irradiation.
Total lymphoid irradiation (TLI) has been reported to be effective in the immunosuppressive treatment of certain human and experimental autoimmune disorders. We have investigated the effects of TLI in Lewis rats with experimental autoimmune myasthenia gravis (EAMG) produced by immunization with purified torpedo acetylcholine receptor (AChR). The radiation is given in 17 divided fractions of 200 rad each, and nonlymphoid tissues are protected by lead shielding. This technique suppresses the immune system, while minimizing side effects, and permits the repopulation of the immune system by the patient's own bone marrow cells. Our results show that TLI treatment completely prevented the primary antibody response to immunization with torpedo AChR, it rapidly abolished the ongoing antibody response in established EAMG, and it suppressed the secondary (anamnestic) response to a boost of AChR. No EAMG animals died during TLI treatment, compared with six control animals that died of EAMG. TLI produces powerful and prompt immunosuppression and may eventually prove useful in the treatment of refractory human myasthenia gravis.
de Silva
S
S
Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
Blum
J E
JE
McIntosh
K R
KR
Order
S
S
Drachman
D B
DB
D000818:Animals; D000917:Antibody Formation; D001327:Autoimmune Diseases / Q000532:radiotherapy*; D004195:Disease Models, Animal; D005260:Female; D007156:Immunologic Memory; D007165:Immunosuppression Therapy; D007700:Kinetics; D008221:Lymphoid Tissue / Q000528:radiation effects*; D009157:Myasthenia Gravis / Q000532:radiotherapy*; D051381:Rats; D011917:Rats, Inbred Lew; D011950:Receptors, Cholinergic / Q000276:immunology*
D011950:Receptors, Cholinergic