Copper-67-labeled monoclonal antibody Lym-1, a potential radiopharmaceutical for cancer therapy: labeling and biodistribution in RAJI tumored mice.
Copper-67 (67Cu) is one of the most promising radiometals for radioimmunotherapy because of its 61.5 hr physical half-life, abundant beta particles, and gamma emissions suitable for imaging. However, 67Cu is readily transferred from the usual chelates of EDTA or DTPA to albumen. We developed a new macrocycle (6-p-nitrobenzyl-TETA) to chelate copper. Bifunctional chelating agent p-bromoacetamidobenzyl-TETA was conjugated to Lym-1, a monoclonal antibody against human B cell lymphoma, without significantly altering its immunoreactivity. This conjugate was stably labeled with 67Cu under conditions chosen to optimize the yield of a high specific activity radiopharmaceutical. The biodistribution in RAJI tumor bearing mice demonstrated significant tumor uptake (14.7% ID per gram) and extended residence time (120 hr) in contrast to normal organs. After 24 hr, radioactivity was continuously cleared from all tissues except the tumor. This study suggests 67Cu labeled Lym-1 to be a promising radiopharmaceutical for potential use for radioimmunotherapy of B cell lymphoma.
Deshpande
S V
SV
Department of Radiology (Nuclear Medicine) University of California, Davis Medical Center, Sacramento 95817.
DeNardo
S J
SJ
Meares
C F
CF
McCall
M J
MJ
Adams
G P
GP
Moi
M K
MK
DeNardo
G L
GL
D000818:Animals; D000911:Antibodies, Monoclonal / Q000627:therapeutic use*; D001402:B-Lymphocytes; D003301:Copper Radioisotopes / Q000627:therapeutic use*; D005260:Female; D007553:Isotope Labeling; D008223:Lymphoma / Q000276:immunology / Q000378:metabolism / Q000532:radiotherapy*; D051379:Mice; D014018:Tissue Distribution
D000911:Antibodies, Monoclonal; D003301:Copper Radioisotopes