Destruction of ethidium bromide in solution by ozonolysis.
Ethidium bromide can be rapidly destroyed in aqueous solutions or in isoamyl alcohol by ozonolysis in the presence of H2O2 to give a mixture of organic acids. In a variety of buffers commonly used in recombinant DNA technology destruction of ethidium bromide was more than 99.9%. The yellow reaction mixture after ozonolysis was shown to be nonmutagenic. This method may be used in laboratories for the disposal of ethidium bromide wastes.
Zocher
R
R
Institut für Biochemie und Molekulare Biologie der Technischen Universität Berlin.
Billich
A
A
Keller
U
U
Messner
P
P
D055598:Chemical Phenomena; D002621:Chemistry; D004996:Ethidium; D006861:Hydrogen Peroxide; D007700:Kinetics; D010126:Ozone; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet
D012996:Solutions; D010126:Ozone; D006861:Hydrogen Peroxide; D004996:Ethidium