On-line sample processing and analysis of diol compounds in biological fluids.
We developed a coupled dual column system with an optional post-column derivatization for on-line sample processing, trace enrichment and analysis of aromatic 1,2-diol and aliphatic cis-diol biomolecules (e.g. catecholamines, ribonucleosides). The fully automated high-performance liquid chromatography analyzer tolerates the direct injection of proteinaceous fluids by use of a unique bonded-phase precolumn material which allows the simultaneous performance of covalent affinity and size-exclusion chromatography.
Boos
K S
KS
Laboratory for Biological Chemistry, University, Paderborn, F.R.G.
Wilmers
B
B
Schlimme
E
E
Sauerbrey
R
R
D002395:Catecholamines / Q000032:analysis* / Q000097:blood / Q000652:urine; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D008895:Milk, Human / Q000032:analysis; D012263:Ribonucleosides / Q000032:analysis* / Q000097:blood / Q000652:urine; D013050:Spectrometry, Fluorescence
D002395:Catecholamines; D012263:Ribonucleosides