High-performance liquid chromatographic determination of netilmicin in guinea-pig and human serum by fluorodinitrobenzene derivatization with spectrophotometric detection.
A high-performance liquid chromatographic procedure for netilmicin determination in guinea-pig and human serum using pre-column derivatization with 1-fluoro-2,4-dinitrobenzene and UV detection is described. Linearity was established over the range 0.5-40 micrograms/ml using only 50 microliters of serum. Accuracy and precision were good, with a mean coefficient of variation less than 5% and a mean relative error less than 4%. This procedure correlates well with an enzyme multiplied immunoassay technique and has a sensitivity similar to those of published fluorescence derivatization methods.
Dionisotti
S
S
Research Laboratories, Essex Italia (Subsidiary of Schering-Plough, Italy.
Bamonte
F
F
Gamba
M
M
Ongini
E
E
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004139:Dinitrofluorobenzene; D004423:Ear / Q000187:drug effects; D006168:Guinea Pigs; D006801:Humans; D007202:Indicators and Reagents; D009428:Netilmicin / Q000097:blood* / Q000633:toxicity; D013056:Spectrophotometry, Ultraviolet
D007202:Indicators and Reagents; D009428:Netilmicin; D004139:Dinitrofluorobenzene