title: An immunohistochemical study performed with monoclonal and polyclonal antibodies to mouse epidermal growth factor.
abstract: We developed two monoclonal antibodies, E5 and F5, which react with mouse epidermal growth factor (mEGF) and urogastrone. These antibodies and a rabbit antiserum to mEGF were used for immunohistochemical analysis of staining reactions in rodent and human tissues. Our results do not confirm the published reports of EGF-like immunoreactivity in Brunner's glands, human submandibular glands, human kidney tissue, or rodent brain sections.
authors.affiliation: Department of Pathology, University of Leiden, The Netherlands.
authors.forename: H J
authors.initials: HJ
authors.lastname: Beerstecher
authors.forename: C
authors.initials: C
authors.lastname: Huiskens-van der Meij
authors.forename: S O
authors.initials: SO
authors.lastname: Warnaar
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001923:Brain Chemistry; D002011:Brunner Glands / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D004815:Epidermal Growth Factor / Q000276:immunology*; D006801:Humans; D006825:Hybridomas; D007106:Immune Sera / Q000276:immunology; D007118:Immunoassay; D007150:Immunohistochemistry; D007668:Kidney / Q000032:analysis; D051379:Mice; D051381:Rats; D013363:Submandibular Gland / Q000032:analysis
chemical_list: D000911:Antibodies, Monoclonal; D007106:Immune Sera; D004815:Epidermal Growth Factor