title: In vivo dynamics of CD4-8- thymocytes. Proliferation, renewal and differentiation of different cell subsets studied by DNA biosynthetic labeling and surface antigen detection.
abstract: The proliferative status of CD4-8- thymocyte subsets was determined by in vivo or in vitro labeling with bromodeoxyuridine (BrdUrd), a nonreutilized thymidine analogue detectable with a monoclonal antibody, simultaneously with relevant surface proteins. An actively cycling subset [J11d+, interleukin 2 receptor-positive (IL 2R+)] was defined, besides a relatively resting one (J11d-, Pgp1+, T cell receptor-positive). Continuous per os administration of BrdUrd showed that 85% only of CD4-8- thymocytes were labeled in 6 days confirming the existence of a relatively long-term resting subset. By contrast, CD4+8+ thymocytes were all labeled in 3-4 days. Observation of labeled CD4-8- cells after pulse labeling showed an immediate decrease of their absolute number per thymus, confirming their low autorenewal capacity. However, a small number of labeled cells which were hydroxyurea or colchicine resistant remained CD4-8- for several days and progressively acquired surface expression of IL 2R. IL 2R expression by cycling CD4-8- cells during thymus regeneration after antimitogenic drug treatment was rapid, but very transient. According to these results most CD4-8- thymocytes appear as largely engaged in a proliferation-dependent differentiative process, and do not behave as true stem cells. Consequently, this subset is principally renewed by thymic immigration of exogenously produced resting cells. However, a tenfold expansion of CD4-8- cells was found in the fetal and regenerating thymus, suggesting two proliferative phases during intrathymic CD4-8- cell maturation, the first one yielding to cell expansion and the second to cell differentiation. A tentative evaluation of daily cell immigration is proposed starting with the determination of the number of cells beginning DNA synthesis each day. A global model is finally discussed by confronting our kinetic results with the known reconstitution capacities of CD4-8- thymocyte subsets.
authors.affiliation: Immunologie Clinique, CNRS UA 122, INSERM U25, Hôpital Necker, Paris, France.
authors.forename: C
authors.initials: C
authors.lastname: Penit
authors.forename: F
authors.initials: F
authors.lastname: Vasseur
authors.forename: M
authors.initials: M
authors.lastname: Papiernik
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D002453:Cell Cycle; D002454:Cell Differentiation; D002470:Cell Survival; D004247:DNA / Q000096:biosynthesis; D051379:Mice; D015375:Receptors, Interleukin-2 / Q000032:analysis; D013234:Stem Cells / Q000166:cytology; D013601:T-Lymphocytes / Q000166:cytology*; D013950:Thymus Gland / Q000166:cytology*
chemical_list: D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D015375:Receptors, Interleukin-2; D004247:DNA