Oxidative detection of coulometrically reduced organonitro pesticides in reversed-phase high-performance liquid chromatography.
A high-performance liquid chromatographic system is presented for determination of organonitro pesticides, metabolites and industrial chemicals having a variety of chemical structures. The compounds are chromatographed on a C8 column with an acetonitrile-aqueous ammonium monochloroacetate gradient. The chromatographic responses of the compounds are monitored indirectly by oxidative detection of the coulometrically reduced organonitro functionality by means of a porousgraphite detector. Three nitrophenols (2,4-dinitrophenol, 4,6-dinitro-o-cresol and dinoseb), a dinitrophenyl aliphatic ester (binapacryl) and a nitroaniline (dicloran) were detected at low nanogram levels (3-10 ng for 50% recorder full-scale deflection). This technique overcomes the problems of high background currents and oxygen interference, observed in the direct reductive detection of organonitro compounds.
Krause
R T
RT
Division of Contaminants Chemistry, Food and Drug Administration, Washington, DC 20204.
Wang
Y
Y
D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D009574:Nitro Compounds / Q000032:analysis*; D010084:Oxidation-Reduction; D010575:Pesticides / Q000032:analysis*
D009574:Nitro Compounds; D010575:Pesticides