title: Effect of the bile acid taurolithocholate on cell calcium in saponin-treated rat hepatocytes.
abstract: Neomycin was used to assess the involvement of Ins (1,4,5)P3 in the Ca2+ release from the endoplasmic reticulum induced by the bile acid taurolithocholate. In saponin-permeabilized rat hepatocytes, neomycin via its ability to bind Ins (1,4,5)P3 abolished the release of Ca2+ induced by added Ins (1,4,5)P3. In contrast, it did not alter the Ca2+ release initiated by the bile acid. In intact cells, neomycin had no effect on the [Ca2+]i rises promoted by taurolithocholate and vasopressin. It is suggested that the effect of taurolithocholate in liver is not mediated by Ins (1,4,5)P3 but results from a primary action on endoplasmic reticulum.
authors.affiliation: Unité de Recherche INSERM U.274, Université Paris-Sud Bât. 443, Orsay, France.
authors.forename: L
authors.initials: L
authors.lastname: Combettes
authors.forename: M
authors.initials: M
authors.lastname: Dumont
authors.forename: B
authors.initials: B
authors.lastname: Berthon
authors.forename: S
authors.initials: S
authors.lastname: Erlinger
authors.forename: M
authors.initials: M
authors.lastname: Claret
mesh_terms: D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D002478:Cells, Cultured; D004987:Ethers / Q000494:pharmacology; D015544:Inositol 1,4,5-Trisphosphate; D007295:Inositol Phosphates / Q000494:pharmacology; D015759:Ionomycin; D007700:Kinetics; D008095:Lithocholic Acid / Q000031:analogs & derivatives*; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D009355:Neomycin / Q000494:pharmacology; D051381:Rats; D012503:Saponins / Q000494:pharmacology*; D013658:Taurolithocholic Acid / Q000494:pharmacology*; D014667:Vasopressins / Q000494:pharmacology
chemical_list: D004987:Ethers; D007295:Inositol Phosphates; D012503:Saponins; D014667:Vasopressins; D013658:Taurolithocholic Acid; D015759:Ionomycin; D008095:Lithocholic Acid; D015544:Inositol 1,4,5-Trisphosphate; D009355:Neomycin; D002118:Calcium