[Changes in the mitotic cycle of Chinese hamster cells and the repair of radiation damage in cells following the action of ionizing radiation].
Using DNA flow cytofluorimetry technique, the effect of ionizing and gamma irradiation on cell cycle in Chinese hamster cells of clone CHO K1 773 and of its derivative EBR-30 resistant to ethidium bromide was examined. Irradiation in doses of 1 and 5.5 Gr leads to a reduced rate of cell passing from G1 into S phase, to a prolonged S phase and to a larger postsynthetic block in EBR-30 cells than in 773 cells. Our data correlate with some delay in repair of gamma-induced (200 Gr) DNA single-strand breaks in the EBR-30 clone. Clones under investigation are not distinguished in their survival. Our results may arise from deficiency in some regulatory process usually involved in cell response to radiation-induced lesions.
Kirillova
T V
TV
Martynova
I M
IM
Rozanov
Iu M
IuM
Seregina
T B
TB
D000818:Animals; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000187:drug effects / Q000528:radiation effects*; D004249:DNA Damage; D004260:DNA Repair / Q000187:drug effects / Q000528:radiation effects*; D004277:DNA, Single-Stranded / Q000187:drug effects / Q000528:radiation effects; D004351:Drug Resistance; D004996:Ethidium / Q000037:antagonists & inhibitors / Q000494:pharmacology; D005434:Flow Cytometry; D005720:Gamma Rays; D008938:Mitosis / Q000187:drug effects / Q000528:radiation effects*; D014965:X-Rays
D004277:DNA, Single-Stranded; D004247:DNA; D004996:Ethidium
Izmenenie mitoticheskogo tsikla kletok kitaĭskogo khomiachka i reparatsiia radiatsionnykh povrezhdeniĭ kletok posle deĭstviia ioniziruiushchego izlucheniia.