Conformation-dependent recognition of a protein by T-lymphocytes: apomyoglobin-specific T-cell clone recognizes conformational changes between apomyoglobin and myoglobin.
A T-cell clone specific to apomyoglobin was generated. It was prepared from a T-cell culture obtained by in vitro driving of lymph node cells with apomyoglobin from SJL mice that have been primed in vivo with apomyoglobin. In proliferative assays, the T-cell clone responded to apomyoglobin but did not recognize native myoglobin or any of the synthetic peptides corresponding to the six T sites of myoglobin. The demonstration that a T-cell clone can be isolated, whose specificity is directed entirely to apomyoglobin and not to its counterpart myoglobin, with an identical amino acid composition, indicates the importance of the three-dimensional structure in the presentation of the protein to T cells.
Cohly
H H
HH
NASA-Johnson Space Center, Houston, Texas 77058.
Morrison
D R
DR
Atassi
M Z
MZ
D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology; D000941:Antigens; D001059:Apoproteins / Q000276:immunology*; D002999:Clone Cells / Q000276:immunology; D051379:Mice; D009211:Myoglobin / Q000276:immunology*; D011487:Protein Conformation; D013601:T-Lymphocytes / Q000276:immunology*
D000941:Antigens; D001059:Apoproteins; D009211:Myoglobin; C010857:apomyoglobin