Purification to homogeneity and biochemical characterization of two suppressor factors from human malignant T-cells.
A cell clone (GI-CO-T-9) derived from a long term T-cell culture (PF-382), established from a patient affected by acute T-lymphoblastic leukemia (T-ALL), was selected for the presence in the culture medium of factors suppressing T-cell proliferation. The crude supernatant has been subjected to a multi-step chromatographic fractioning, including: preparative gel permeation, anion exchange, and hydrophobic interaction High Performance Liquid Chromatography (HPLC). The highly purified material was characterized by polyacrylamide gel electrophoresis in sodium dodecyl sulfate (SDS-PAGE), revealing single bands of 115 Kd and 80 Kd. The isoelectric points (pI), determined by flat-bed isoelectric-focusing, were 7.4 for High Molecular Weight Suppressor Factor (HMWSF) and 3.5-3.6 for Low Molecular Weight Suppressor Factor (LMWSF).
Ponzoni
M
M
Pediatric Oncology Research Laboratory, G. Gaslini Children's Hospital, Italy.
Montaldo
P G
PG
Lanciotti
M
M
Castagnola
E
E
Cirillo
C
C
Cornaglia-Ferraris
P
P
D002460:Cell Line; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D006801:Humans; D007945:Leukemia, Lymphoid / Q000276:immunology; D008970:Molecular Weight; D013491:Suppressor Factors, Immunologic / Q000302:isolation & purification*; D013601:T-Lymphocytes / Q000276:immunology*
D013491:Suppressor Factors, Immunologic