Preparative-scale high-performance liquid chromatography of omega-3 polyunsaturated fatty acid esters derived from fish oil.
Marine triglyceride-derived omega-3 polyunsaturated fatty acid ethyl esters were separated by preparative high-performance liquid chromatography on a 25-microns octadecyl stationary phase using a ternary isocratic mobile phase of acetonitrile-tetrahydrofuran-water (466:233:300, v/v/v). The highest purity first-run fractions obtained were ethyl esters of the major marine polyunsaturates eicosapentaenoic acid (20:5 omega 3, 97.7%) and docosahexaenoic acid (22:6 omega 3, 93.7%), and the minor polyunsaturate octadecatetraenoic acid (18:4 omega 3, 98.1%).
Beebe
J M
JM
Department of Chemistry, University of Rhode Island, Kingston 02881.
Brown
P R
PR
Turcotte
J G
JG
D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D005231:Fatty Acids, Unsaturated / Q000302:isolation & purification*; D005395:Fish Oils / Q000032:analysis*; D006801:Humans
D005231:Fatty Acids, Unsaturated; D005395:Fish Oils