title: Tertiary structure of mouse epidermal growth factor determined by two-dimensional 1H NMR.
abstract: The tertiary structure of mouse epidermal growth factor (EGF) in solution (28 degrees C, pH 2.0) was studied by two-dimensional NMR spectroscopy. Proton-proton distance constraints derived from NOESY spectra were used to construct a mechanical molecular model of mouse EGF, which was subsequently checked by means of a preliminary distance geometry calculation. The chain-folds in the two structural domains of mouse EGF were very similar to those previously reported (Montelione et al. (1987) Proc. Natl. Acad. Sci. U.S. 84, 5226-5230). However, the relative orientations of the two domains were different. Because we could assign much more inter-domain NOEs, the relative orientations of the two domains were well determined in our model. The hollow between the two domains may function as a binding site for the EGF receptor.
authors.affiliation: Department of Molecular Physiology, Tokyo Metropolitan Institute of Medical Science.
authors.forename: D
authors.initials: D
authors.lastname: Kohda
authors.forename: N
authors.initials: N
authors.lastname: Go
authors.forename: K
authors.initials: K
authors.lastname: Hayashi
authors.forename: F
authors.initials: F
authors.lastname: Inagaki
mesh_terms: D000818:Animals; D004815:Epidermal Growth Factor; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008297:Male; D051379:Mice; D008958:Models, Molecular; D011487:Protein Conformation
chemical_list: D004815:Epidermal Growth Factor