Automated purification of human protein band 3, the major integral protein of the erythrocyte membrane.
Human erythrocyte protein band 3 was purified from a Triton X-100 extract of white ghosts. This purification, including an ion-exchange chromatography and a group-affinity chromatography, was automated. The apparatus was assembled from commercially available elements and allowed for the recovery of 2 to 3 mg pure band 3 in 2 hr. The purification could be repeated several times a day. The advantages of automation are discussed.
Vasseur
C
C
INSERM U 299, Hopital de Bicêtre, France.
Truskolaski
A
A
Bertrand
O
O
Bursaux
E
E
D001457:Anion Exchange Protein 1, Erythrocyte / Q000302:isolation & purification*; D001322:Autoanalysis / Q000295:instrumentation*; D002846:Chromatography, Affinity / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D004910:Erythrocyte Membrane / Q000032:analysis*; D006801:Humans; D008565:Membrane Proteins / Q000032:analysis*
D001457:Anion Exchange Protein 1, Erythrocyte; D008565:Membrane Proteins