Preparation of human cord sera for enzymatic triglyceride assays: removal of free glycerol by ultrafiltration.
Enzymatic triglyceride assays that generate glycerol from triglycerides as a part of the enzymatic process in quantitating serum triglyceride levels give elevated values when external free glycerol is present. Our objective was to develop an ultrafiltration technique that would remove exogenous and/or endogenous free glycerol from small aliquots of human cord sera so that accurate serum triglyceride values could be obtained with the commercially available triglyceride assay kits. Exogenous glycerol was completely removed from cord sera when the samples were washed twice with saline in Amicon Centricon-30 microconcentrators. This ultrafiltration technique lowered cord serum triglyceride levels significantly (P less than 0.001), but had no effect on cord total serum cholesterol levels. A comparison of washed and unwashed cord sera by either polyacrylamide or agarose gel electrophoresis indicated that the serum protein and lipoprotein profiles were not altered by the ultrafiltration process.
Khan
I M
IM
Department of Chemistry and Biochemistry, California State University, Los Angeles 90032.
Schwartz
J J
JJ
Woo
D
D
Garcia
R E
RE
D002784:Cholesterol / Q000097:blood; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005312:Fetal Blood / Q000032:analysis*; D005990:Glycerol / Q000097:blood; D006801:Humans; D007231:Infant, Newborn; D008049:Lipase / Q000378:metabolism; D011933:Reagent Kits, Diagnostic; D014280:Triglycerides / Q000097:blood*; D014462:Ultrafiltration / Q000379:methods*
D011933:Reagent Kits, Diagnostic; D014280:Triglycerides; D002784:Cholesterol; D008049:Lipase; D005990:Glycerol