title: Metabolic time course and immunologic concomitants of adoptive transfer of type I diabetes in the BB rat.
abstract: The metabolic and cellular immune changes during adoptive transfer of type I diabetes mellitus in the BB rat were examined. Concanavalin A (Sigma Chemical Co, St Louis) stimulation of acutely diabetic BB rat splenocytes increased the Ia-positive cells, but no other lymphocyte subset. Each spleen cell preparation was divided into two and injected into two separate recipients. Thirty-day-old diabetes-prone BB rats received these splenocytes intravenously (62 +/- 5 x 10(6) cells, n = 30) or buffer alone (controls, n = 14). Seventy-seven percent of the cell-injected rats became diabetic before 60 days of age, 15 +/- 1 days after injection. They were glucose intolerant two to three days before onset, with normal fasting glucose. All controls maintained normal glucose tolerance. The morphology revealed intense insulitis in all the rats that became diabetic, and its absence in all the controls. Eighty-three percent of the spleen cell preparations produced the same outcome in both recipients. The cell-injected rats had an increase in lymphocyte counts eight days after injection compared with the controls. The most affected subsets were the pan T cells (OX19+) and helper T cells (W3/25+). While the rats that ultimately became diabetic had a decrease of their lymphocyte subsets to control levels between eight and 14 days, the injected rats that maintained normal glucose tolerance maintained elevated T cells. We conclude that (1) adoptive transfer of diabetes occurs in the presence of an increase of the helper T (W3/25+) lymphocytes after spleen cell injections; (2) glucose intolerance precedes by two to three days fasting hyperglycemia; and (3) while the lymphocyte counts are increased in all recipients of splenocyte preparations, these counts decrease rapidly only in the rats that develop diabetes, possibly by entrapment of lymphocytes in the insulitis.
authors.affiliation: McGill Nutrition and Food Science Centre, Royal Victoria Hospital, Montreal, Canada.
authors.forename: J F
authors.initials: JF
authors.lastname: Yale
authors.forename: T A
authors.initials: TA
authors.lastname: Seemayer
authors.forename: C
authors.initials: C
authors.lastname: Vigeant
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000378:metabolism*; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000276:immunology*; D003922:Diabetes Mellitus, Type 1 / Q000097:blood / Q000276:immunology*; D005951:Glucose Tolerance Test; D007116:Immunization, Passive; D051381:Rats; D011910:Rats, Brattleboro; D013601:T-Lymphocytes / Q000145:classification / Q000276:immunology*
chemical_list: D001786:Blood Glucose