2-dimensional flow cytometric analysis of peripheral blood T lymphocytes from patients with systemic lupus erythematosus: preferential expression of HLA-DR antigen on the surface of Leu 2a+ cells.
2-dimensional flow cytometric analysis of the peripheral blood T lymphocytes from 20 patients with systemic lupus erythematosus showed a significant increase in the percentage of Leu 2a+ 15- cytotoxic subset irrespective of disease activity compared to 35 age and sex matched healthy individuals. The percentage of Leu 2a+ HLA-DR + cells also increased significantly in both patients with active and inactive disease compared to the controls. In contrast, both the relative and absolute number of Leu 3a+ 8+ cell (suppressor/inducer T cell) showed a significant reduction in active and inactive disease if compared to controls. All of these changes were more drastic in the patients with active disease.
Tsuchiya
N
N
Department of Medicine and Physical Therapy, Faculty of Medicine, University of Tokyo, Japan.
Mitamura
T
T
Goto
M
M
Moroi
Y
Y
Kinoshita
M
M
Yokohari
R
R
Miyamoto
T
T
D000328:Adult; D000906:Antibodies / Q000502:physiology; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis*; D002462:Cell Membrane / Q000276:immunology; D005434:Flow Cytometry / Q000379:methods*; D006681:HLA-D Antigens / Q000032:analysis*; D006684:HLA-DR Antigens / Q000032:analysis*; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000097:blood / Q000188:drug therapy / Q000276:immunology*; D008181:Lupus Nephritis / Q000097:blood / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology; D008875:Middle Aged; D011239:Prednisolone / Q000627:therapeutic use; D013601:T-Lymphocytes / Q000145:classification / Q000276:immunology*
D000906:Antibodies; D000945:Antigens, Differentiation, T-Lymphocyte; D006681:HLA-D Antigens; D006684:HLA-DR Antigens; D011239:Prednisolone