The measurement of warfarin enantiomers in serum using coupled achiral/chiral, high-performance liquid chromatography (HPLC).
An assay for the serum concentration of the enantiomers of warfarin, R-warfarin and S-warfarin, has been developed using a bovine serum albumin chiral stationary phase (BSA-CSP) coupled to a Pinkerton internal-surface reverse-phase (ISRP) achiral column. The ISRP column is used to separate R,S-warfarin from the serum components and warfarin metabolites and to quantitate the total R,S-warfarin concentration. The eluent containing R,S-warfarin is then selectively transferred to the BSA-CSP, where the enantiomers are stereochemically resolved (alpha = 1.19) and the enantiomeric composition is determined. This system is sensitive and accurate, does not require extensive precolumn manipulations, and can be automated for use in large-scale clinical studies.
Chu
Y Q
YQ
National Institute for the Control of Pharmaceutical and Biological Products, Beijing, China.
Wainer
I W
IW
D002851:Chromatography, High Pressure Liquid; D006801:Humans; D011422:Propionates / Q000493:pharmacokinetics; D011485:Protein Binding; D012710:Serum Albumin, Bovine / Q000378:metabolism; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism; D014859:Warfarin / Q000031:analogs & derivatives / Q000097:blood* / Q000493:pharmacokinetics
D011422:Propionates; C011677:benoxaprofen; D012710:Serum Albumin, Bovine; D014859:Warfarin