Optical measurements of intracellular pH in intact isolated muscle fibres and muscle growth cones in culture.
Intracellular pH (pHi) has been measured in intact muscle fibres isolated from the mouse flexor digitorum brevis muscle, using the fluorescence of dicyanohydroquinone as an indicator. The pHi in fibres cultured from 1 to 14 days ranged from 7.0 to 8.1. With time in culture, isolated fibres developed growth cone-like extensions from their ends. These 'myocones' were significantly more alkaline by 0.1-0.2 pH units than the parent fibre. Cultured fibres regulated pHi in response to acid loading with an NH4Cl pulse with recovery rate constants of up to 0.8 pH units/min.
Gillespie
J I
JI
Department of Physiological Sciences, University of Newcastle.
Ribchester
R R
RR
D000818:Animals; D001826:Body Fluids / Q000378:metabolism*; D046508:Culture Techniques; D005260:Female; D006863:Hydrogen-Ion Concentration; D007424:Intracellular Fluid / Q000378:metabolism*; D051379:Mice; D008808:Mice, Inbred CBA; D024510:Muscle Development; D009132:Muscles / Q000378:metabolism*