Liquid chromatographic determination of aflatoxins in feedstuffs containing citrus pulp.
A liquid chromatographic (LC) method was developed for the determination of aflatoxins in feedstuffs containing citrus pulp. The feed-stuff sample is extracted with chloroform, followed by Sep-Pak Florisil cartridge cleanup and Sep-Pak C18 cartridge cleanup. The final eluate (water-acetone, 85 + 15, v/v) is submitted to reverse-phase liquid chromatography with water-methanol-acetonitrile (130 + 70 + 40, v/v/v) as mobile phase and postcolumn derivatization with iodine. Citrus components are removed from the extract efficiently. The limit of detection for aflatoxin B1 is less than 1 microgram/kg. Other aflatoxins can also be detected and measured. Recoveries of aflatoxins B1, B2, G1, and G2 for dairy rations spiked at 13, 5, 10, and 4 micrograms/kg were 87, 86, 81, and 82%, respectively. Corresponding coefficients of variation were 3.1, 3.6, 5.2, and 3.8%, respectively.
Paulsch
W E
WE
National Institute of Public Health and Environmental Protection, Laboratory for Residue Analysis, Bilthoven, The Netherlands.
Sizoo
E A
EA
van Egmond
H P
HP
D000348:Aflatoxins / Q000032:analysis*; D000821:Animal Feed / Q000032:analysis; D002855:Chromatography, Thin Layer; D002957:Citrus / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis*
D000348:Aflatoxins