Laboratory markers for over-attenuation of mumps vaccine virus.
The properties of mumps vaccine virus (Leningrad-3 strain) gradually changed upon passaging in quail embryo fibroblasts, the substrate normally used for mumps vaccine production in the USSR. Alterations were extremely noticeable in the over-attenuated (38th passage) virus variant, and involved (a) poor, if any, antibody response in guinea-pigs, (b) turbid plaque formation, (c) lack of expression in cell culture of fusion protein and reduced expression of polymerase protein, and (d) enrichment by abnormally small, fusion-protein-deficient virus particles. Two other laboratory strains exhibited a similar trend to over-attenuation, though after variable passage numbers. Due to a good inter-correlation, every test (namely, inoculation of guinea-pigs, plaque assay, protein analysis, or immune electron microscopy) is indicative of mumps vaccine over-attenuation and hence might be valuable in seed virus quality control.
Boriskin YuS
Institute for Viral Preparations, Moscow, USSR.
Kaptsova
T I
TI
Lotte
V D
VD
Skvortsova
O I
OI
Orvell
C
C
D000818:Animals; D000937:Antigen-Antibody Reactions; D015415:Biomarkers; D006168:Guinea Pigs; D009109:Mumps virus / Q000276:immunology*; D010316:Particle Size; D014613:Vaccines, Attenuated / Q000032:analysis; D014760:Viral Fusion Proteins; D010948:Viral Plaque Assay; D014765:Viral Vaccines / Q000032:analysis
D015415:Biomarkers; D014613:Vaccines, Attenuated; D014760:Viral Fusion Proteins; D014765:Viral Vaccines