Interaction between thymocytes and thymus-derived macrophages. I. Surface components participating in mutual recognition.
Incubation of C57BL/6 thymus-derived macrophages (TDM phi) with syngeneic thymocytes resulted in binding of thymocytes to macrophages and rosette formation. Up to 60% of the TDM phi formed rosettes with thymocytes after 6 hr of interaction at 4 degrees C. Rosette formation of the immature PNA+ thymocyte fraction was up to fivefold higher than that of PNA- and cortisone-resistant thymocytes. Pretreatment of PNA- thymocytes with neuraminidase enhanced thymocyte binding to macrophages up to sevenfold, whereas a marked reduction of rosette formation was seen following (1) incubation of thymocytes with tunicamycin; (2) incubation of macrophages with 20 mM D-galactose, GLCNaC, or GalNaC; (3) treatment of macrophages or thymocytes with trypsin; (4) treatment of macrophages with anti-1-Ab mAb and its F(ab')2 fragment; (5) treatment of thymocytes with anti-Lyt-2.2 mAb; and (6) addition of EDTA and EGTA to the interacted two cell populations.
Zeira
M
M
Lautenberg Center for General and Tumor Immunology, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
Gallily
R
R
D000818:Animals; D050260:Carbohydrate Metabolism; D002413:Cations, Divalent / Q000502:physiology; D002450:Cell Communication; D002454:Cell Differentiation; D002458:Cell Fractionation; D005260:Female; D037102:Lectins / Q000494:pharmacology; D008264:Macrophages / Q000378:metabolism / Q000502:physiology*; D008565:Membrane Proteins / Q000378:metabolism; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D019887:Peanut Agglutinin; D011485:Protein Binding; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000166:cytology* / Q000378:metabolism; D013950:Thymus Gland / Q000166:cytology; D014415:Tunicamycin / Q000494:pharmacology
D002413:Cations, Divalent; D037102:Lectins; D008565:Membrane Proteins; D019887:Peanut Agglutinin; D014415:Tunicamycin