B cell repertoire for anti-DNA antibody in normal and lupus mice: differential expression of precursor cells for high and low affinity anti-DNA antibodies.
The precursor frequency for anti-DNA antibody producing cells and the affinity of antibodies secreted by these cells in both immature prereceptor B cell populations and mature B cell populations were compared between 8-week-old C57BL/6 female mice and 9-month-old B/WF1 female mice by producing a large collection of IgM secreting hybridomas from LPS-stimulated B cells. The data indicate that precursor cells for high affinity anti-DNA antibody are eliminated as they mature in C57BL/6 mice, while a sizable number of such clones are present in mature splenic B cells of aged B/WF1 mice. These results suggest that the emergence of precursors for high affinity anti-DNA producing cells in mature B cell population is an important factor in the pathogenesis of SLE.
Tsubata
T
T
Second Department of Internal Medicine, Faculty of Medicine, Kyoto University, Japan.
Nishikawa
S
S
Katsura
Y
Y
Kumagai
S
S
Imura
H
H
D000818:Animals; D000974:Antibodies, Antinuclear / Q000096:biosynthesis* / Q000276:immunology; D000915:Antibody Affinity; D001402:B-Lymphocytes / Q000276:immunology* / Q000473:pathology; D002454:Cell Differentiation; D004247:DNA / Q000276:immunology*; D005260:Female; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D013154:Spleen / Q000276:immunology
D000974:Antibodies, Antinuclear; D004247:DNA