Establishment and characterization of a clonal cell line (RPC-C2A) from dental pulp of the rat incisor.
Maxillary incisor pulp cells from male Wistar rats (7 weeks old) were cultured. After the 52nd subculture, cloning was performed twice and a clonal cell line (RPC-C2A) with high alkaline phosphatase (ALP) activity was established. The population doubling time of RPC-C2A cells was 13.7 h, and the mode of the chromosome number was 42. The heat stability of ALP, the effect of the ALP inhibitors, and the ALP polyacrylamide gel electrophoresis pattern in RPC-C2A cells was the same as for the ALP of the isolated dental pulp.
Kasugai
S
S
Department of Pharmacology, Faculty of Dentistry, Tokyo Medical and Dental University, Japan.
Adachi
M
M
Ogura
H
H
D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D002452:Cell Count; D002460:Cell Line; D002875:Chromosomes; D002999:Clone Cells; D003782:Dental Pulp / Q000166:cytology* / Q000201:enzymology; D007180:Incisor; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008938:Mitosis
D000469:Alkaline Phosphatase