Improved transfer of high molecular weight proteins using fabric-reinforced soft polyacrylamide gels.
Rapid transfer of electrophoretically separated, high molecular weight proteins from fabric-reinforced, soft, low-concentration polyacrylamide gels (3.5%T, 2.6%C) is described. Polyester fabrics proved useful for gel reinforcement during vertical slab gel electrophoresis. The fabric-reinforced gel retains its dimension during transfer, equilibration and drying, facilitating comparison of protein bands in the separation gel and transfer matrix.
Starita-Geribaldi
M
M
Laboratoire de Biochimie, Faculté de Médecine Chemin de Valombrose, Nice, France.
Giudicelli
J
J
Sudaka
P
P
D003101:Collodion; D004591:Electrophoresis, Polyacrylamide Gel / Q000295:instrumentation; D008567:Membranes, Artificial; D008970:Molecular Weight; D011091:Polyesters; D011506:Proteins / Q000302:isolation & purification*
D008567:Membranes, Artificial; D011091:Polyesters; D011506:Proteins; D003101:Collodion