Differentiation potential of 14-day fetal mouse thymocytes in organ culture. Analysis of CD4/CD8-defined single-positive and double-negative cells.
A two-color flow cytometric analysis was carried out on 14-day fetal mouse thymus lobes maintained for various lengths of time in organ culture. Kinetic analysis showed that the proportions of CD4- and CD8-defined subpopulations changed with time. In particular, there appeared to be accumulation of J11d-, CD4/CD8 double-negative and J11d-, Ly-6A+ single-positive cells, a phenotype associated with "mature" cells. Gated two-color analysis of CD4/CD8 single-positive cells showed that they contained distinct subpopulations as defined by mAb to CD3, TCR (F23.1), Ly-6A, Ly-6C, LFA-1, and J11d. Thus, the precursor cells found in the 14-day fetal mouse thymus were capable of generating all the subpopulations of CD4/CD8-defined single-positive and double-negative cells seen in the normal adult mouse thymus.
Ceredig
R
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Department of Experimental Pathology, John Curtin School of Medical Research, Canberra City, Australia.
D000818:Animals; D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis*; D002454:Cell Differentiation; D005260:Female; D005333:Fetus / Q000276:immunology*; D005434:Flow Cytometry; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D009924:Organ Culture Techniques; D010641:Phenotype; D012542:Scattering, Radiation; D013601:T-Lymphocytes / Q000145:classification / Q000166:cytology* / Q000276:immunology
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte