[Functional capacity of T-cell subpopulations of the mouse. Effect of unstimulated and allogenic stimulated T-cell subpopulations on Con A stimulation of splenic lymphocytes].
The distribution and the functional capacity of special subpopulations was examined by means of monoclonal anti-Lyt-1.2-, anti-Lyt-2.2-, anti-L3T4- and anti-Thy-1.2-antibodies. The proportion of Lyt-2+-cells increased after allogeneic stimulation of spleen cells compared with the Lyt-1+-cells. The proportion of L3T4+-cells did not change by allogeneic stimulation. Lyt-2- cells which were negatively selected by monoclonal antibody and complement effected the Con A stimulated 3H-thymidine incorporation of a spleen cell culture in a different way depending on their state of differentiation. While Lyt-2--cells selected from a fresh prepared spleen cell suspension increased the 3H thymidine incorporation, Lyt-2--cells selected from allogeneically stimulated cell suspension act as suppressor cells. The results demonstrate the dynamics in the functional capacity of the Lyt-2--T cell subpopulation depending on their state of differentiation.
Hückel
C
C
Institut für Biochemie, Wilhelm-Pieck-Universität Rostock.
Kusnierczyk
P
P
Pajtasz
E
E
Brock
J
J
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology; D003208:Concanavalin A / Q000276:immunology*; D008213:Lymphocyte Activation; D051379:Mice; D008815:Mice, Inbred Strains; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000145:classification / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D003208:Concanavalin A
Untersuchungen zur funktionellen Kapazität von T-Zell-Subpopulationen der Maus. Einfluss von unstimulierten und allogen stimulierten T-Zell-Subpopulationen auf eine Con A-Stimulierung von Milzlymphozyten.