Rapid indirect enzyme linked immunosorbent assay (ELISA) for detecting antitoxoplasma IgG: comparison with dye test.
A rapid and simple enzyme linked immunosorbent assay (ELISA) for the detection of specific IgG against Toxoplasma gondii was compared with the dye test on 533 serum samples. In general, results were comparable but not with sera that contained high concentrations of toxoplasma specific IgM or that had been heated at 56 degrees C. There were no false positive results with sera containing rheumatoid factor or anti-nuclear factor. It is concluded that if a dye test is not to be performed then the serum should be tested for both toxoplasma specific IgG and IgM to avoid misleading results. Heat inactivated serum should also not be tested in this type of specific IgG assay.
Francis
J M
JM
Public Health Laboratory, Singleton Hospital, Swansea.
Payne
R A
RA
Joynson
D H
DH
D000818:Animals; D000974:Antibodies, Antinuclear / Q000276:immunology; D000913:Antibodies, Protozoan / Q000032:analysis*; D004396:Coloring Agents; D004797:Enzyme-Linked Immunosorbent Assay; D005189:False Positive Reactions; D006358:Hot Temperature; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis*; D007075:Immunoglobulin M / Q000032:analysis; D012217:Rheumatoid Factor / Q000276:immunology; D014122:Toxoplasma / Q000276:immunology*
D000974:Antibodies, Antinuclear; D000913:Antibodies, Protozoan; D004396:Coloring Agents; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D012217:Rheumatoid Factor