Fast protein liquid chromatography of lymphocyte blastogenesis inhibitory factor produced by the human macrophage-like cell line U937.
The human macrophage-like cell line U937 constitutively produces a lymphocyte blastogenesis inhibitory factor (LBIF). Using fast protein liquid chromatography (FPLC)-chromatofocusing, FPLC-anion-exchange chromatography and high-performance liquid chromatography-gel permeation chromatography, LBIF has been purified to homogeneity. It is a single polypeptide chain and has a molecular weight of 57,000 daltons according to sodium dodecyl sulphate polyacrylamide gel electrophoresis under reducing conditions. LBIF shows pI 4.28-4.56 upon chromatofocusing on Mono P.
Sugimura
K
K
Institute of Immunological Science, Hokkaido University, Supporo, Japan.
Tsukahara
K
K
Ueda
Y
Y
Takeda
K
K
Habu
Y
Y
Azuma
I
I
D000818:Animals; D002460:Cell Line; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D002853:Chromatography, Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D007375:Interleukin-1 / Q000037:antagonists & inhibitors; D007525:Isoelectric Focusing; D008222:Lymphokines / Q000302:isolation & purification*; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D013601:T-Lymphocytes / Q000187:drug effects
D007375:Interleukin-1; D008222:Lymphokines