Development of an interleukin-2 slow delivery system.
The authors developed a slow delivery system for interleukin-2 (IL-2) using highly purified bovine collagen to overcome the rapid clearance and side effects of IL-2; 1 X 10(6) microns of recombinant human IL-2 was successfully infused into needle-shaped "IL-2 mini-pellets". Pharmacokinetic study in C57BL/6 mice revealed that a single subcutaneous injection of an IL-2 mini-pellet could prolong IL-2 retention and decrease maximal concentration in the serum. Elimination half-life was 360 min for subcutaneously injected IL-2 mini-pellets, while it was 8 and 15 min, respectively, for intravenous and subcutaneous injections of aqueous IL-2. No side effects were observed throughout the experiment. This slow delivery system using collagen as a carrier proved to be effective and may be applicable to other kinds of drugs.
Matsuoka
J
J
Department of Surgery, Okayama University Medical School, Japan.
Sakagami
K
K
Shiozaki
S
S
Uchida
S
S
Fujiwara
T
T
Gohchi
A
A
Orita
K
K
D000818:Animals; D002417:Cattle; D003094:Collagen; D003692:Delayed-Action Preparations; D004337:Drug Carriers; D005260:Female; D006207:Half-Life; D007376:Interleukin-2 / Q000008:administration & dosage* / Q000493:pharmacokinetics; D051379:Mice; D008810:Mice, Inbred C57BL; D011994:Recombinant Proteins / Q000493:pharmacokinetics
D003692:Delayed-Action Preparations; D004337:Drug Carriers; D007376:Interleukin-2; D011994:Recombinant Proteins; D003094:Collagen