Femtomole analysis of diclofenac in human plasma by gas chromatography/negative ion chemical ionization mass spectrometry using (18O2) diclofenac as internal standard.
A stable isotope dilution gas chromatographic/negative ion chemical ionization mass spectrometric assay for diclofenac in human plasma is described. The preparation of (18O2)diclofenac and its use as an internal standard for quantitative gas chromatography/mass spectrometry is shown. A sample processing and derivatization sequence with product recovery of 84.7% was found. The method presented permits quantitative measurement of diclofenac in human plasma at the lower femtomole level. Plasma levels of diclofenac after administration of diclofenac gel were estimated.
Leis
H J
HJ
Univ. Kinderklinik, Abt. Massenspektrometrie, Graz, Austria.
Gleispach
H
H
Nitsche
V
V
Malle
E
E
D004008:Diclofenac / Q000097:blood*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D066298:In Vitro Techniques; D010103:Oxygen Isotopes
D010103:Oxygen Isotopes; D004008:Diclofenac