Preparation of dopamine 3-O-sulphate and dopamine 4-O-sulphate as reference substances and high-performance liquid chromatographic trace determination.
Simple syntheses of the biologically important but hitherto difficult to obtain dopamine sulpho conjugates dopamine 3-O-sulphate (I) and dopamine 4-O-sulphate (II), as analytical reference substances, starting from dopamine hydrochloride are described. A method for the determination of I and II with reversed-phase high-performance liquid chromatographic separation and coulometric detection in human urine together with calibration and current-voltage curves are presented. Detection limits of approximately 100 pg of I or II and unequivocal substance identifications even in very complex substrates such as human urine are reported.
Kienzl
E
E
Ludwig Boltzmann-Institute of Clinical Neurobiology, Vienna, Austria.
Eichinger
K
K
D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D004298:Dopamine / Q000031:analogs & derivatives* / Q000652:urine; D006801:Humans; D012015:Reference Standards; D013056:Spectrophotometry, Ultraviolet
C022807:dopamine 4-O-sulfate; C007558:dopamine 3-O-sulfate; D004298:Dopamine