C-banding as a simple tool to discriminate between micronuclei induced by clastogens and aneugens.
If mouse bone marrow preparations are treated with a classical C-banding procedure, it may be possible to distinguish between micronuclei with or without centromeres. This allows discrimination between micronuclei originating from chromosome breakage and those originating from chromosome loss. Thus, using C-banding, the micronucleus test can be used not only for the detection of clastogens but also aneugens. In this way, more exhaustive methods such as immunological staining using antikinetochore antibodies may not be necessary.
Verschaeve
L
L
Laboratory for Human Genetics, Free University of Brussels (V.U.B), Belgium.
Vanderkerken
K
K
Kirsch-Volders
M
M
D000818:Animals; D001854:Bone Marrow Cells; D002503:Centromere / Q000032:analysis; D002871:Chromosome Banding / Q000379:methods*; D051379:Mice; D015162:Micronucleus Tests / Q000379:methods*; D009153:Mutagens / Q000494:pharmacology*
D009153:Mutagens