Rapid gas-chromatographic microdetermination of soluphylline in serum.
A nitrogen-specific gas-chromatographic method is described for the quantitative analysis of soluphylline without the need for derivatization or evaporation of solvent, and using only 50 microliters of serum. The analysis is performed isothermally on a silicone stationary phase, 2% SP 2250 DA (Supelco). After a single neutral extraction with ethyl acetate containing the internal standard (beta-hydroxypropyl theophylline), an aliquot of the organic solvent is injected directly into the gas chromatograph. The detector sensitivity (least measurable amount) for soluphylline was 1 ng.
Arranz Peña
M I
MI
Biochemistry Service of Ramon y Cajal Hospital, Madrid.
Saenz Lope
E
E
D001993:Bronchodilator Agents / Q000097:blood*; D002849:Chromatography, Gas / Q000379:methods; D006801:Humans; D007202:Indicators and Reagents; D008832:Microchemistry; D012997:Solvents; D013806:Theophylline / Q000031:analogs & derivatives* / Q000097:blood
D001993:Bronchodilator Agents; D007202:Indicators and Reagents; D012997:Solvents; D013806:Theophylline; C008749:etofylline