Direct comparison of electric field-mediated and PEG-mediated cell fusion for the generation of antibody producing hybridomas.
As an alternative to polyethylene glycol (PEG), electric field pulses offer, in theory, fusion conditions whose parameters are better controllable. In 1985 (1) we reported on the successful generation of hybridoma clones by means of electrofusion performed in a batch-type manner similar to that usually employed with PEG, and applicable to any type of antigens. Here we summarize the results of a series of fusions performed since then in which both electric field and PEG induced fusion were directly compared. Different types of antigens were used. Electrofusion resulted in a 3.8 to 33.0 times higher yield of hybridomas per unit number of spleen cells. Moreover, hybridomas grew more vigorously after fusion and, therefore, were earlier visible. Other parameters examined revealed no differences between hybridomas generated by either method.
Karsten
U
U
Central Institute of Molecular Biology, Academy of Sciences of the GDR, Berlin-Buch.
Stolley
P
P
Walther
I
I
Papsdorf
G
G
Weber
S
S
Conrad
K
K
Pasternak
L
L
Kopp
J
J
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D002459:Cell Fusion; D004574:Electromagnetic Fields; D055590:Electromagnetic Phenomena; D006801:Humans; D006825:Hybridomas / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D011092:Polyethylene Glycols / Q000494:pharmacology*; D051381:Rats; D011916:Rats, Inbred F344; D013154:Spleen / Q000166:cytology / Q000187:drug effects
D000911:Antibodies, Monoclonal; D011092:Polyethylene Glycols