Inhibitors of sterol synthesis. Studies of the distribution and metabolism of 5 alpha-[2,4-3H]cholest-8(14)-en-3 beta-ol-15-one after intragastric administration to rats.
5 alpha-[2,4-3H]Cholest-8(14)-en-3 beta-ol-15-one was administered to a series of male Sprague-Dawley rats by intragastric intubation in the form of an emulsion in a mixture of triolein, sodium taurocholate, bovine serum albumin, and glucose. [4-14C]Cholesterol was similarly administered to a second series of rats. The distribution of 3H and 14C was studied at 12 and 48 h after the administration of the sterols. The results demonstrated that the 15-ketosterol is absorbed and metabolized to material with the chromatographic properties of fatty acid esters of the 15-ketosterol, to cholesterol, and to fatty acid esters of cholesterol. The [3H]cholesterol formed from the 15-ketosterol was characterized by its behavior on silicic acid-Super Cel column chromatography, by the chromatographic behavior of its acetate derivative on alumina-AgNO3 column chromatography, and by purification by way of its dibromide derivative without significant change in specific activity. The general distribution of 3H was similar to that of 14C. No unusual concentration of 3H in any of the organs studied was observed.
Brabson
J S
JS
Department of Biochemistry, Rice University, Houston, TX 77251.
Schroepfer
G J
GJ
D000818:Animals; D000924:Anticholesteremic Agents / Q000008:administration & dosage / Q000378:metabolism*; D002782:Cholestenes / Q000378:metabolism*; D002783:Cholestenones / Q000008:administration & dosage / Q000378:metabolism*; D007441:Intubation, Gastrointestinal; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013261:Sterols / Q000096:biosynthesis*
D000924:Anticholesteremic Agents; D002782:Cholestenes; D002783:Cholestenones; D013261:Sterols; C015073:cholest-8(14)-en-3-ol-15-one