Interferon-alpha can synergize with interleukin 2 for human in vitro antibody response.
We tested the effect of interferon (IFN)-alpha on the specific anti-2,4,6-trinitrophenyl (TNP) response induced by TNP-conjugated polyacrylamide beads in cultures of purified human B cells. IFN-alpha alone had no effect. Interleukin 2 (IL2) alone had a restorative effect which could be considerably (10X) enhanced by IFN-alpha. This reflected a true synergy most apparent with IL2 concentrations of 10 U/ml and IFN-alpha concentrations of 10(3)-10(5) U/ml. The highest concentrations of IFN-alpha were not inhibitory. In contrast, IFN-alpha did not enhance the effect of an IL2-free T cell-derived supernatant able to support the B cell differentiation. Sequential incubations showed that IFN-alpha acted earlier than IL2 on B cell response. The effect of IFN-alpha was dependent on an efficient interaction between IL2 and its receptor which could be inhibited by a monoclonal antibody toward the CD25 antigen. Thus, IFN-alpha can positively interact with a well defined interleukin, IL2, at a pre or post receptor level to potentiate antibody response.
Delfraissy
J F
JF
INSERM U131, Clamart, France.
Wallon
C
C
Galanaud
P
P
D000917:Antibody Formation / Q000187:drug effects*; D000921:Antibody-Producing Cells / Q000166:cytology; D001402:B-Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D004357:Drug Synergism; D006801:Humans; D066298:In Vitro Techniques; D007370:Interferon Type I / Q000008:administration & dosage / Q000494:pharmacology*; D007376:Interleukin-2 / Q000008:administration & dosage / Q000494:pharmacology*; D015375:Receptors, Interleukin-2 / Q000502:physiology; D011994:Recombinant Proteins; D013997:Time Factors
D007370:Interferon Type I; D007376:Interleukin-2; D015375:Receptors, Interleukin-2; D011994:Recombinant Proteins