Regulation of hybridoma B-cell proliferation by immunoglobulin-binding factor.
Immunoglobulin-binding factors (IBF), which are cytokines able to suppress the immunoglobulin production by normal and transformed B cells, have been tested for their ability to interfere with hybridoma B-cell growth. Coculture experiments performed in soft agar between hybridoma B cells and hybridoma T cells secreting IgG-BF indicated that IgG-BF act as a growth regulatory molecule able to inhibit strongly the development of hybridoma B-cell colonies; this cytostatic effect, which appeared not to be a direct cytotoxic effect, was confirmed when using semi-purified IgG-BF in both soft agar and liquid medium cultures of hybridoma B cells. Thus, IBF appear to act as both immunoregulatory and growth regulatory factors. Some of the molecular consequences of this dual effect and its possible role in certain B-cell lymphoproliferative diseases such as multiple myeloma are discussed.
Teillaud
J L
JL
Laboratoire d'Immunologie Cellulaire et Clinique, Unité INSERM 255, Institut Curie, Paris, France.
Mathiot
C
C
Amigorena
S
S
Brunati
S
S
Moncuit
J
J
Fridman
W H
WH
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D002455:Cell Division / Q000187:drug effects; D003470:Culture Media; D006825:Hybridomas / Q000276:immunology*; D007074:Immunoglobulin G / Q000276:immunology; D007526:Isoelectric Point; D008222:Lymphokines / Q000494:pharmacology*; D051379:Mice; D009101:Multiple Myeloma / Q000276:immunology; D009336:Necrosis; D029584:Prostatic Secretory Proteins
D003470:Culture Media; D007074:Immunoglobulin G; D008222:Lymphokines; D029584:Prostatic Secretory Proteins; C043076:beta-microseminoprotein; C013059:immunoglobulin-binding factors