Assessment of persisting chromosome aberrations by flow karyotyping of cloned Chinese hamster cells.
A preparation, staining and measuring protocol for high resolution flow cytometry of chromosomes was developed. This method allows us to identify all chromosome types and is suited for characterization of permanent cell lines and cell clones by establishing their flow karyotypes. In cell clones this procedure can be used for the detection of chromosomal aberrations which appear spontaneously or are induced by mutagen treatment and persist in the cell population.
Otto
F J
FJ
Abteilung für experimentelle Tumorforschung Fachklinik Hornheide, Münster, Bundesrepublik Deutschland.
D000818:Animals; D002460:Cell Line; D002869:Chromosome Aberrations; D002999:Clone Cells; D006224:Cricetinae; D003412:Cricetulus; D005434:Flow Cytometry / Q000379:methods; D007621:Karyotyping