Analysis of tissue glycoprotein sugar chains by two-dimensional high-performance liquid chromatographic mapping.
Excellent separation of 45 pyridylamino derivatives of oligosaccharides were achieved by the two-dimensional combination of reversed-phase and size-fractionation high-performance liquid chromatography. The sugar chains of brain glycoproteins were derivatized into a mixture of pyridylamino-oligosaccharides from lyophilized brain tissue without any purification steps, and they were well separated by the system used. The pattern obtained was reproducible, and inter-individual variation was negligible. This finding demonstrated the possibility that this method could be applied to the detection of differences in the structure of glycoprotein sugar chains in crude preparations.
Hase
S
S
Department of Chemistry, Osaka University, College of Science, Japan.
Ikenaka
K
K
Mikoshiba
K
K
Ikenaka
T
T
D000818:Animals; D001923:Brain Chemistry; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D006023:Glycoproteins / Q000032:analysis*; D051379:Mice; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000032:analysis*
D006023:Glycoproteins; D009844:Oligosaccharides