title: A human lymphoblastoid B line with an abnormally large MHC class II beta chain.
abstract: We have examined the MHC class II beta chains in lymphoblastoid cell lines from over 200 individuals and describe one line which possesses, in addition to normal beta chains, a species of beta chain of unusually high Mr and abnormal pI which appears to be a product of the DR locus. This abnormality in Mr, detected by SDS-gel electrophoresis, was apparent only in the presence of mercaptoethanol and was shown to be due to difference in polypeptide chain length rather than to extra glycosylation.
authors.affiliation: Clinical and Population Cytogenetics Unit, Western General Hospital, Edinburgh, U.K.
authors.forename: B B
authors.initials: BB
authors.lastname: Cohen
authors.forename: D N
authors.initials: DN
authors.lastname: Crichton
mesh_terms: D001402:B-Lymphocytes / Q000276:immunology*; D002460:Cell Line; D006026:Glycoside Hydrolases; D006681:HLA-D Antigens / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D007122:Immunoelectrophoresis; D008623:Mercaptoethanol; D008970:Molecular Weight; D011487:Protein Conformation
chemical_list: D006681:HLA-D Antigens; D008623:Mercaptoethanol; D006026:Glycoside Hydrolases