title: Generation of multiple troponin T isoforms is a common feature of the muscles in various chordate animals.
abstract: 1. Troponin T (TNT) expressed in various vertebrate skeletal and ascidian smooth muscles was examined by two-dimensional electrophoresis in combination with immunoblotting. 2. A monoclonal anti-TNT antibody, NT-302, exhibited binding ability to various TNT variants in the vertebrate and protochordate animals. 3. TNT isoform pattern differed among the animals, but the existence of multiple TNT isoforms in a single muscle tissue was the general feature of all the animals examined.
authors.affiliation: Department of Biology, Faculty of Science, Chiba University, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Ohshima
authors.forename: T
authors.initials: T
authors.lastname: Komiya
authors.forename: K
authors.initials: K
authors.lastname: Takeuchi
authors.forename: T
authors.initials: T
authors.lastname: Endo
authors.forename: T
authors.initials: T
authors.lastname: Obinata
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D002816:Chordata, Nonvertebrate / Q000235:genetics / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D014644:Genetic Variation; D015151:Immunoblotting; D009130:Muscle, Smooth / Q000032:analysis; D009132:Muscles / Q000032:analysis*; D014336:Troponin / Q000276:immunology / Q000302:isolation & purification*
chemical_list: D000911:Antibodies, Monoclonal; D014336:Troponin