Inhibition of lipopolysaccharide activation of 7OZ/3 cells by anti-lipopolysaccharide antibodies.
We have investigated the ability of mAb against LPS to inhibit LPS-induced activation of 7OZ/3 pre-B cells. The fine specificity and relative affinity of these mAb for lipid A and LPS were also determined. We found that antibodies inhibited only the activity of glycolipids which they bound with relatively high affinity. However, two high affinity antibodies binding to non-lipid A epitopes did not block cellular activation. Some, but not all, relatively high affinity antibodies binding to the lipid A region of the LPS molecule inhibited biologic activity. The inhibitory antibodies bound to at least two distinct epitopes within the lipid A region. These data suggest that LPS interacts with 7OZ/3 cells in a highly specific fashion.
Kirkland
T N
TN
Department of Pathology, University of California, San Diego 92161.
Ziegler
E J
EJ
Tobias
P
P
Ward
D C
DC
Michalek
S M
SM
McGhee
J R
JR
Macher
I
I
Urayama
K
K
Appelmelk
B J
BJ
D000818:Animals; D000907:Antibodies, Bacterial / Q000502:physiology; D000911:Antibodies, Monoclonal / Q000502:physiology*; D000915:Antibody Affinity; D000918:Antibody Specificity; D001402:B-Lymphocytes / Q000276:immunology*; D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D002460:Cell Line; D008050:Lipid A / Q000037:antagonists & inhibitors / Q000276:immunology / Q000378:metabolism; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors / Q000276:immunology / Q000378:metabolism; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D014411:Neoplastic Stem Cells
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D008050:Lipid A; D008070:Lipopolysaccharides