title: Determination of pterins in urine by high-performance liquid chromatography on C18 columns conditioned with cetyltrimethylammonium bromide.
abstract: A method is proposed for the determination of pterins in urine without clean-up by reversed-phase high-performance liquid chromatography on a C18 column previously conditioned with cetyltrimethylammonium bromide. Other endogenous compounds are retained in the column, preventing interference. Xanthopterin, neopterin, isoxanthopterin, monapterin, biopterin, 6-hydroxymethylpterin, pterin and lumazine can be determined. Retention and separation mechanisms are discussed.
authors.affiliation: Facultad de Ciencias Quimicas, Departamento de Quimica Analítica, Universidad Complutense de Madrid, Spain.
authors.forename: B C
authors.initials: BC
authors.lastname: Montalvo
authors.forename: C I
authors.initials: CI
authors.lastname: Villar
authors.forename: R C
authors.initials: RC
authors.lastname: Hornillos
authors.forename: L P
authors.initials: LP
authors.lastname: Diez
mesh_terms: D000077286:Cetrimonium; D002593:Cetrimonium Compounds; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D011622:Pterins / Q000652:urine*; D013050:Spectrometry, Fluorescence
chemical_list: D002593:Cetrimonium Compounds; D007202:Indicators and Reagents; D011622:Pterins; D000077286:Cetrimonium