Interleukin-1 and tumor necrosis factor production by tumor-associated mononuclear leukocytes and peripheral mononuclear leukocytes in cancer patients.
The production of interleukin-1 (IL-1) and tumor necrosis factor (TNF) by tumor-associated mononuclear leukocytes (TAML) and peripheral mononuclear leukocytes (PMBL) from 9 otherwise untreated patients with a variety of malignancies (lung, sarcoma, stomach, renal) was assessed. Cells were cultured for 24 hr in vitro in the presence or absence of lipopolysaccharide (LPS), and IL-1 and TNF levels were measured in culture supernatants. TNF production was comparable between TAMLs and PBMLs. However, a striking defect in IL-1 production by TAMLs was noted. There was no basal production of IL-1 and LPS-stimulated TAMLs produced only 1% the amount of IL-1 produced by LPS-stimulated PBMLs.
Economou
J S
JS
Division of Surgical Oncology, Armand Hammer Laboratories, Jonsson Comprehensive Cancer Center, UCLA Medical Center 90024.
Colquhoun
S D
SD
Anderson
T M
TM
McBride
W W
WW
Golub
S
S
Holmes
E C
EC
Morton
D L
DL
D001681:Biological Assay / Q000379:methods; D002478:Cells, Cultured; D006801:Humans; D007375:Interleukin-1 / Q000096:biosynthesis*; D007963:Leukocytes, Mononuclear / Q000187:drug effects*; D008070:Lipopolysaccharides / Q000494:pharmacology; D009369:Neoplasms / Q000097:blood*; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
D007375:Interleukin-1; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha