Effect of chemical modification with p-bromophenacyl bromide on the enzymatic and lethal properties of phospholipase A2 from Bothrops alternatus (Víbora de la Cruz) venom.
The effects on lethal potency and enzymatic activity were determined following alkylation, with p-bromophenacyl bromide, of the acidic toxic phospholipase A2 from Bothrops alternatus. The modified B. alternatus enzyme, which lost its enzymatic activity, retained considerable toxicity. Histopathologic studies on mice have demonstrated features similar to those of the native enzyme. However, the distribution of the damage was different and the survival time was longer. It is concluded that the enzyme activity is not important for the lethal action of the enzyme although it influences the distribution of the damage and survival time.
Nisenbom
H E
HE
Instituto de Neurobiología (IDNEU), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina.
Perazzo
J C
JC
Monserrat
A J
AJ
Vidal
J C
JC
D000098:Acetophenones / Q000494:pharmacology*; D000818:Animals; D008099:Liver / Q000187:drug effects / Q000473:pathology; D051379:Mice; D009206:Myocardium / Q000473:pathology; D010740:Phospholipases / Q000633:toxicity*; D010741:Phospholipases A / Q000037:antagonists & inhibitors / Q000633:toxicity*; D054467:Phospholipases A2; D011650:Pulmonary Alveoli / Q000187:drug effects / Q000473:pathology; D014757:Viper Venoms / Q000633:toxicity*
D000098:Acetophenones; D014757:Viper Venoms; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2; C006821:4-bromophenacyl bromide