High-performance immunoaffinity chromatographic detection of immunoregulatory anti-idiotypic antibodies in cancer patients receiving immunotherapy.
Anti-idiotypic antibodies are regulatory antibodies responsible for the shutdown of active immune responses against growing tumor cells. In an attempt to study these antibodies, a technique for isolating specific anti-idiotypic antibodies by immunoaffinity chromatography was devised. Human anti-tumor antibodies were isolated by affinity absorption to fixed autologous tumor cells. These antibodies were biotinylated, immobilized on streptavidin-coated beads, and used as a ligand to isolate reactive anti-idiotypes from the plasma of patients during periods when immune reactivity against their tumors could not be detected. The isolated anti-idiotypes demonstrated the ability to react with the original antibodies and to inhibit their binding to autologous tumor cells. Thus functional anti-idiotypic antibodies can be isolated by immunoaffinity chromatography with the original idiotype as the ligand. This technique can be used to monitor regulatory antibodies in cancer patients receiving immunotherapy.
Phillips
T M
TM
Department of Medicine, George Washington University Medical Center, Washington, DC 20037.
D000888:Antibodies, Anti-Idiotypic / Q000032:analysis*; D000912:Antibodies, Neoplasm / Q000032:analysis / Q000276:immunology; D002851:Chromatography, High Pressure Liquid; D003602:Cytotoxicity, Immunologic; D006801:Humans; D007114:Immunization; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D007167:Immunotherapy; D009101:Multiple Myeloma / Q000276:immunology* / Q000628:therapy
D000888:Antibodies, Anti-Idiotypic; D000912:Antibodies, Neoplasm; D007130:Immunoglobulin Idiotypes