[Growth-stimulating, cytostatic and cytotoxic activity of the secretory products of the RPMI-6410t human B-cell lymphoblastoid line].
The cells of human lymphoblastoid line RPMI-6410t were shown to synthesize constitutively a factor(s) with different types of biological activity. The factor(s) stimulated the growth of both B cells 6410t, obtained from the blood of a patient with acute myeloblastic leukemia, and the human embryonic diploid fibroblasts. With B cell lines Raji and P3HR-1.G5, obtained from the patients with Burkitt's lymphoma. The growth factor(s) displayed cytotoxic and cytostatic effects, respectively. Growth-stimulating and cytotoxic activating of the factor were destroyed by a 15 hour exposure to low or high pH. The activity was stable within pH values of 6-8. With regard to heat stability, the activity destroyed at 70 degrees C within 1 hour but remained stable at 56 degrees C during 1 hour. The above factor(s) displayed biological activities similar to those of the previously known tumor necrosis factor (TNF).
Seregina
T M
TM
Mekshenkov
M I
MI
D001402:B-Lymphocytes / Q000187:drug effects / Q000473:pathology / Q000502:physiology*; D002051:Burkitt Lymphoma / Q000188:drug therapy / Q000473:pathology; D002461:Cell Line, Transformed; D002471:Cell Transformation, Neoplastic / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D002999:Clone Cells / Q000187:drug effects / Q000473:pathology / Q000502:physiology; D004353:Drug Evaluation, Preclinical; D004355:Drug Stability; D006133:Growth Substances / Q000096:biosynthesis / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D015470:Leukemia, Myeloid, Acute / Q000188:drug therapy / Q000473:pathology; D013696:Temperature; D013997:Time Factors; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000378:metabolism / Q000494:pharmacology*
D006133:Growth Substances; D014409:Tumor Necrosis Factor-alpha
Roststimuliruiushchaia, tsitostaticheskaia i tsitotoksicheskaia aktivnost' sekretornykh produktov limfoblastnoĭ linii B-kletok cheloveka RPMI-6410t.