Degeneration and regrowth of adrenergic nerves after microvascular anastomosis. A fluorescence histochemical study on end-to-end anastomoses of femoral vessels in the rat.
The normal femoral artery and its branches were found to be innervated with a dense network of adrenergic nerves. The nerve plexus around the vein was sparse. Adventitial stripping of the femoral vessels, with or without division and reanastomosis, caused local disappearance of catecholamine fluorescence in the stripped area. The distal adrenergic innervation remained normal if the femoral nerve was left intact. Division of the femoral nerve, alone or in combination with blood vessel division and reanastomosis, caused total disappearance of catecholamine fluorescence from the adrenergic nerves of the entire distal neurovascular tree examined. At the end of the observation period of 36 weeks from the time of division of the nerve, artery and vein with subsequent microvascular anastomosis, numerous adrenergic nerves were observed to have crossed the suture line. The vascular nerve plexus around the femoral vessels was dense in places, but in other places sparse or absent. It seems that the reinnervation occurs not only over the suture line, but also together with other regenerating nerves from the adjacent tissues and by collateral sprouting from adjacent adrenergically normally innervated areas.
Waris
T
T
Department of Surgery, Oulu University Central Hospital, Finland.
Hukki
J
J
Lähteenmäki
T
T
Husa
T
T
Bäck
N
N
van Smitten
K
K
D000320:Adrenergic Fibers / Q000502:physiology*; D000714:Anastomosis, Surgical; D000818:Animals; D002395:Catecholamines / Q000032:analysis; D005263:Femoral Artery / Q000294:innervation / Q000601:surgery*; D005267:Femoral Nerve / Q000502:physiology; D005268:Femoral Vein / Q000294:innervation / Q000601:surgery*; D008856:Microscopy, Fluorescence; D008866:Microsurgery; D009410:Nerve Degeneration; D009416:Nerve Regeneration; D051381:Rats
D002395:Catecholamines