Antigen preservation in infarcted lymphoid tissue. A novel approach to the infarcted lymph node using monoclonal antibodies effective in routinely processed tissues.
We studied 11 cases of malignant lymphoma diagnosed concurrently with or following lymph node infarction. Cases included seven B-cell lymphomas, three T-cell lymphomas, and one case of Hodgkin's disease. Sections of viable and infarcted tissue were immunostained in parallel using a panel of antibodies effective in routinely processed, wax-embedded tissue. The panel included anti-leucocyte-common antigen (CD45), T-cell-associated antigens (UCHL1, MT1), B-cell-associated antigens (MB1, 4KB5 (CD45R), MT2, LN1), a B-cell-specific antigen (L26), C3D-1 (CD15), and BER-H2 (CD30). Antibodies to intermediate filament cytoskeletal proteins, epithelial membrane antigen, and Factor VIII-related antigen were also used. In eight cases, staining of the infarcted material gave evidence of a lymphoid proliferation of either T- or B-cell type; an in the case of Hodgkin's disease, the results supported this diagnosis. The immunophenotype derived in the infarcted tissue mirrored the findings in the viable material in these eight cases of non-Hodgkin's lymphoma. A case of testicular infarction with concurrent intraosseous lymphoma was also examined. Staining in this case provided evidence of infarcted lymphoma. Thus, immunostaining of infarcted lymphoid tissue with these novel antibodies provides valuable information that conventional light microscopy cannot offer.
Norton
A J
AJ
Department of Histopathology, University College and Middlesex Medical School, London, England.
Ramsay
A D
AD
Isaacson
P G
PG
D000911:Antibodies, Monoclonal; D000937:Antigen-Antibody Reactions; D001402:B-Lymphocytes; D006649:Histocompatibility Antigens / Q000276:immunology; D006689:Hodgkin Disease / Q000276:immunology / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D007238:Infarction / Q000150:complications / Q000276:immunology / Q000473:pathology*; D008198:Lymph Nodes / Q000098:blood supply*; D008223:Lymphoma / Q000150:complications / Q000276:immunology / Q000473:pathology*; D013601:T-Lymphocytes
D000911:Antibodies, Monoclonal; D006649:Histocompatibility Antigens