Synthesis and rapid purification of UDP-[6-3H]galactose.
UDP[6-3H]galactose of high specificity can be obtained by oxidation of the C-6 hydroxymethyl group of UDP-galactose by galactose oxidase and subsequent reduction by sodium borotritide. One-step purification of the nucleotide sugar involves anion-exchange chromatography on a Pharmacia Mono Q column. Radiolabeled UDP-N-acetylgalactosamine can also be synthesized and purified by this procedure. Both nucleotide sugars can be used for sugar incorporation studies using the appropriate glycosyltransferase.
Fudem-Goldin
B
B
Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, NY 10461.
Voulalas
P
P
Orr
G A
GA
D002852:Chromatography, Ion Exchange / Q000379:methods; D014316:Tritium; D014531:Uridine Diphosphate Galactose / Q000138:chemical synthesis* / Q000302:isolation & purification; D014539:Uridine Diphosphate Sugars / Q000138:chemical synthesis*
D014539:Uridine Diphosphate Sugars; D014316:Tritium; D014531:Uridine Diphosphate Galactose