Tertiary structure of mouse epidermal growth factor determined by two-dimensional 1H NMR.
The tertiary structure of mouse epidermal growth factor (EGF) in solution (28 degrees C, pH 2.0) was studied by two-dimensional NMR spectroscopy. Proton-proton distance constraints derived from NOESY spectra were used to construct a mechanical molecular model of mouse EGF, which was subsequently checked by means of a preliminary distance geometry calculation. The chain-folds in the two structural domains of mouse EGF were very similar to those previously reported (Montelione et al. (1987) Proc. Natl. Acad. Sci. U.S. 84, 5226-5230). However, the relative orientations of the two domains were different. Because we could assign much more inter-domain NOEs, the relative orientations of the two domains were well determined in our model. The hollow between the two domains may function as a binding site for the EGF receptor.
Kohda
D
D
Department of Molecular Physiology, Tokyo Metropolitan Institute of Medical Science.
Go
N
N
Hayashi
K
K
Inagaki
F
F
D000818:Animals; D004815:Epidermal Growth Factor; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008297:Male; D051379:Mice; D008958:Models, Molecular; D011487:Protein Conformation
D004815:Epidermal Growth Factor