Cadmium and zinc concentrations in fetal and maternal rat tissue after parenteral administration of cadmium during pregnancy.
Cadmium (Cd) and zinc (Zn) concentrations were determined by solid sampling atomic absorption spectrometry (AAS) in rat maternal and fetal tissues after exposure to cadmium. Cadmium was administered subcutaneously as CdCl2 in saline daily during pregnancy. Two experiments were performed. In expt. I we investigated the tissue concentration at day 19 (gestational age) after administration of several doses: 0, 1.1, 2.2, 4.4, and 8.8 mumol Cd/kg/day. In expt. II the course of the Cd and Zn concentrations during pregnancy was investigated by collecting samples at days 14, 16, 18 and 20, after daily injections of 4.4 mumol Cd/kg. Cadmium concentrations in blood, maternal liver, placenta and fetal liver increased with dose and duration of exposure. Cadmium was heavily accumulated in the liver and transferred to the fetus only in small amounts. The zinc concentration in the maternal liver was positively correlated with the cadmium concentration. In the placenta the zinc concentration was not affected. Zinc in fetal liver was decreased from day 18 onward. Despite relatively high cadmium levels and decreased zinc levels in the fetus, we observed no adverse effects on various reproduction parameters, such as birth weights and obvious malformations.
Hazelhoff Roelfzema
W
W
Laboratory of Histology and Cell Biology, University of Amsterdam, The Netherland.
Roelofsen
A M
AM
Herber
R F
RF
Peereboom-Steg
J H
JH
D000818:Animals; D002104:Cadmium / Q000097:blood / Q000378:metabolism*; D002105:Cadmium Poisoning / Q000378:metabolism*; D005260:Female; D005333:Fetus / Q000378:metabolism*; D008099:Liver / Q000196:embryology / Q000378:metabolism; D008297:Male; D010920:Placenta / Q000378:metabolism; D011247:Pregnancy; D011248:Pregnancy Complications / Q000378:metabolism*; D051381:Rats; D015032:Zinc / Q000097:blood / Q000378:metabolism*
D002104:Cadmium; D015032:Zinc