Optimization of the ion-pair high-performance liquid chromatographic separation of purine derivatives in erythrocytes, thymocytes and liver mitochondria.
Various methods are described for the analysis of purine derivatives in biological samples by ion-pair high-performance liquid chromatography (HPLC) with both gradient and isocratic systems. A new approach is proposed that is suitable for the separation of nuclei acid constituents in different cells with a specific enzymatic activity pattern. The ion-pair HPLC methods were developed for the analysis of erythrocytes, lymphocytes and mitochondria acid-soluble fractions in clinical and experimental studies of normal and altered nucleotide metabolism. The results of studies of purine metabolite redistribution in mouse liver mitochondria during a 30-min incubation at 37 degrees C and data on purine metabolic alterations in mouse thymocytes during hepatoma growth are discussed.
Toguzov
R T
RT
Department of Biochemistry, Central Research Laboratory, N.I. Pirogov 2nd Moscow Medical Institute, U.S.S.R.
Tikhonov
Y V
YV
Pimenov
A M
AM
Prokudin VYu
Dubiel
W
W
Ziegler
M
M
Gerber
G
G
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004912:Erythrocytes / Q000032:analysis*; D005260:Female; D005311:Fetal Hypoxia / Q000097:blood; D006801:Humans; D007042:Hypoxanthines / Q000097:blood / Q000302:isolation & purification; D007231:Infant, Newborn; D008297:Male; D051379:Mice; D008930:Mitochondria, Liver / Q000032:analysis*; D011247:Pregnancy; D011684:Purine Nucleosides / Q000097:blood / Q000302:isolation & purification; D011687:Purines / Q000097:blood / Q000302:isolation & purification*; D013950:Thymus Gland / Q000032:analysis* / Q000378:metabolism
D007042:Hypoxanthines; D011684:Purine Nucleosides; D011687:Purines