Activation of protein kinase C from B lymphocytes by lipid A.
The effect of lipid A, a residue of the lipopolysaccharide molecule, on protein kinase C from B lymphocytes has been studied. Lipid A activates and promotes the translocation of protein kinase C from the soluble to the particulate membrane fraction in a cell-free system reconstituted with purified enzyme and membranes isolated from B lymphocytes. These results demonstrate that the activating effect of lipopolysaccharide on protein kinase C from B cells is due to the lipid moieties of this molecule.
Boscá
L
L
Instituto de Bioquímica, Facultad de Farmacia, Universidad Complutense, Madrid, Spain.
Díaz-Guerra
M J
MJ
D000818:Animals; D001402:B-Lymphocytes / Q000201:enzymology*; D004789:Enzyme Activation; D007700:Kinetics; D008050:Lipid A / Q000494:pharmacology*; D051379:Mice; D008815:Mice, Inbred Strains; D011493:Protein Kinase C / Q000302:isolation & purification / Q000378:metabolism*
D008050:Lipid A; D011493:Protein Kinase C