Demonstration of leukocyte-immobilizing activity in normal human plasma.
The effect of normal human plasma on random migration of polymorphonuclear leukocytes (PMNs) in agarose micro-droplet assay was studied. Plasma, even at a concentration as low as 1%, inhibits leukocyte motility and this immobilizing activity seems to be related to a specific factor which present in human sera and is stable at 56 degrees C for 30 min. The immobilizing effect of heat-inactivated sera was abolished in vitro by indomethacin at a concentration of 0.1 micrograms/ml and it was significantly decreased by anti PGE2 antibody. Spontaneous migration of leukocytes failed to be enhanced by indomethacin (0.1 microgram/ml) alone. Anti-PGE2 alone moderately enhanced PMN motility. Immobilizing activity in human sera decreased 2 hours after indomethacin administration (Indomethacinum CHINOIN, 50 mg orally). No direct enhancing effect of indomethacin on leukocyte motility was shown in vivo. The data indicate a leukocyte-immobilizing activity of human sera. This effect is mediated by prostaglandin production induced by serum factor(s). The factor(s) responsible for the above activity may have a role in regulating leukocyte distribution and motility in vivo.
Kalmár
L
L
Second Department of Medicine, Semmelweis University Medical School, Budapest, Hungary.
Kádár
J
J
Gergely
P
P
Fehér
J
J
D002465:Cell Movement / Q000187:drug effects; D015232:Dinoprostone / Q000276:immunology; D006801:Humans; D007106:Immune Sera; D066298:In Vitro Techniques; D007213:Indomethacin / Q000494:pharmacology; D007700:Kinetics; D008222:Lymphokines / Q000097:blood / Q000302:isolation & purification / Q000502:physiology*; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology*
D007106:Immune Sera; D008222:Lymphokines; C010598:leukocyte inhibitory factor; D015232:Dinoprostone; D007213:Indomethacin