title: Ultrastructural studies of a lysosomal enzyme during lymphocyte activation.
abstract: A post-embedding immunogold technique has been used for the ultrastructural localization of a lysosomal enzyme, beta-glucuronidase, in resting and activated T- and B-lymphocytes. The results presented here show that mitogen-induced stimulation of T- and B-cells was associated with an increase in the amount of enzyme in the Golgi complex and rough endoplasmic reticulum, organelles which were rarely present in the resting lymphocytes.
authors.affiliation: Department of Histopathology, Charing Cross and Westminster Medical School, London, UK.
authors.forename: G
authors.initials: G
authors.lastname: Bou-Gharios
authors.forename: J
authors.initials: J
authors.lastname: Moss
authors.forename: D
authors.initials: D
authors.lastname: Abraham
authors.forename: T
authors.initials: T
authors.lastname: Partridge
authors.forename: I
authors.initials: I
authors.lastname: Olsen
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000201:enzymology / Q000648:ultrastructure; D004721:Endoplasmic Reticulum / Q000201:enzymology; D005966:Glucuronidase / Q000032:analysis*; D006056:Golgi Apparatus / Q000201:enzymology; D008213:Lymphocyte Activation; D051379:Mice; D008808:Mice, Inbred CBA; D008854:Microscopy, Electron; D013601:T-Lymphocytes / Q000201:enzymology / Q000648:ultrastructure
chemical_list: D005966:Glucuronidase