Determination of nitrogen content in milk by the Kjeldahl method using copper sulfate: interlaboratory study.
Copper sulfate was substituted for mercury as the catalyst in the International Dairy Federation (IDF) Standard 20A:1986 method for the determination of nitrogen content in milk. The substitution was supported by results obtained in an interlaboratory study by 24 laboratories in 12 countries. Each laboratory analyzed 12 test samples of milk as blind duplicates in a double split level design with high, medium, and low nitrogen concentrations. The method protocol requires the concurrent analyses of an ammonium salt solution and a tryptophan solution as internal quality control standards with a minimum nitrogen recovery between 99 and 100% for the former and at least 98% for the latter. The repeatability and reproducibility relative standard deviations are 0.5 and 1%, respectively, for the range 0.35-0.70 g N/100 g. The performance of the laboratories that did not meet the required quality control specifications was clearly poorer than that of those that did meet the specifications.
Grappin
R
R
Institut National de la Recherche Agronomique, Poligny, France.
Horwitz
W
W
D000818:Animals; D002417:Cattle; D003300:Copper; D019327:Copper Sulfate; D007202:Indicators and Reagents; D008892:Milk / Q000032:analysis*; D009584:Nitrogen / Q000032:analysis*; D000644:Quaternary Ammonium Compounds / Q000032:analysis; D014364:Tryptophan / Q000032:analysis
D007202:Indicators and Reagents; D000644:Quaternary Ammonium Compounds; D003300:Copper; D014364:Tryptophan; D019327:Copper Sulfate; D009584:Nitrogen