Application of a new ultra-microculture system. II. Stimulation of human B lymphocytes.
An ultra-microtechnique for culturing human B-lymphocytes in glass capillary tubes using a volume of 2 microliter is described. The advantage of this ultra-microculture system is that only a small number of lymphocytes and minute amounts of culture medium (or test factors) are required. Optimal culture conditions for the formation of Ig-secreting plaque-forming cells (PFC) after stimulation of mononuclear cells with pokeweed mitogen are given. Furthermore it is shown that immunoglobulin secreted into culture supernatants by purified B cells in the presence of T cell subsets can be measured in a microELISA.
Ulmer
A J
AJ
Forschungsinstitut Borstel, F.R.G.
Gruber
M
M
Flad
H D
HD
D000917:Antibody Formation; D000921:Antibody-Producing Cells / Q000166:cytology; D001402:B-Lymphocytes / Q000166:cytology*; D002478:Cells, Cultured; D006801:Humans; D066298:In Vitro Techniques; D008213:Lymphocyte Activation; D008211:Lymphocyte Cooperation; D011043:Pokeweed Mitogens
D011043:Pokeweed Mitogens