[Fundamental studies on the measurement of plasma concentration of oxytocin during perinatal period].
This is an attempt to establish an easy but reliable method for the measurement of the plasma concentration of oxytocin (OT) during the perinatal period. Samples of blood from pregnant and puerperal women were taken by venipuncture and collected in prechilled vacutainers containing EDTA and ophenanthroline. Acidified plasmas were loaded into a ODS-silica column (SEP-PAK). Rinsing of the column and elution of OT were performed with 5ml of 0.1M acetic acid and 3ml of methanol, respectively. The optimal incubation condition for RIA was 25 degrees C for 24 hours. Dextran charcoal was preferable to second antibody (DASP) for bound/free separation of 125I-OT. The recovery rate for plasma OT levels was 95%. Changes in the OT levels in maternal plasma during labor induced with OT infusion and neonatal suckling in postpartum were well demonstrated by the extracts. Radioimmunoactivity of OT in the plasma extract was observed in exactly the same fraction as that of standard OT on high performance liquid chromatography (HPLC).
Furuya
K
K
Department of Obstetrics and Gynecology, National Defense Medical College, Saitama.
Nagata
I
I
Imaizumi
E
E
Hirata
J
J
Kato
K
K
D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D007743:Labor, Obstetric / Q000097:blood; D007774:Lactation / Q000097:blood; D010121:Oxytocin / Q000097:blood*; D049590:Postpartum Period / Q000097:blood*; D011237:Predictive Value of Tests; D011247:Pregnancy / Q000097:blood*; D011863:Radioimmunoassay / Q000379:methods
D010121:Oxytocin