Two-dimensional electrophoresis as a tool for structural and genetic studies of seed proteins from Poaceae and Fagaceae.
The application of two-dimensional electrophoretic procedures to structural and genetic studies of seed proteins from Poaceae (including the cultivated cereals) and Fagaceae is described. The following related problems have been considered: covalent and non-covalent association of protein subunits in multiple oligomeric structures; chromosomal locations of genes encoding seed proteins; quantitation of gene products in relation to gene expression and regulation; purification of protein components to study their homology relationships and in vitro activities; evolutionary and phylogenetic relationships; identification of genetic stocks. Isoelectric focusing, pore-gradient electrophoresis, electrophoresis at different pH's, are among the separation procedures used in the first dimension, whereas sodium dodecyl sulfate-polyacrylamide gel electrophoresis and starch-gel electrophoresis at acid pH have been the preferred second-dimensional methods. Dissociating conditions (sodium dodecyl sulfate, Nonidet P-40, or urea) and reducing conditions (2-mercaptoethanol) have been used when required.
Garcia-Olmedo
F
F
Programa de Biotecnologia, Agroforestal, Universidad Politécnica de Madrid, Spain.
Salcedo
G
G
Aragoncillo
C
C
Sánchez-Monge
R
R
Collada
C
C
Gómez
L
L
D000782:Aneuploidy; D002874:Chromosome Mapping; D002523:Edible Grain / Q000032:analysis* / Q000235:genetics; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D005786:Gene Expression Regulation; D005796:Genes; D001467:Hordeum / Q000032:analysis / Q000235:genetics; D010802:Phylogeny; D010940:Plant Proteins / Q000032:analysis / Q000235:genetics*; D011487:Protein Conformation; D012639:Seeds / Q000032:analysis; D014908:Triticum / Q000032:analysis / Q000235:genetics
D010940:Plant Proteins