Two quick methods for isolation of ribulose-1,5-bisphosphate carboxylase/oxygenase.
Methods are described which allow the isolation of Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase (rubisco) in a very short time. Source of the material was highly impure commercial enzyme in the case of spinach rubisco or bacteria grown from a fermentor in the case of Alcaligenes eutrophus rubisco. Purity of the enzymes is demonstrated by gel electrophoreses. Enzyme isolated from fresh cells gave crystals of excellent diffraction, suitable for X-ray structure analyisis.
Jakob
R
R
Labsystems GmbH, München.
D000421:Alcaligenes / Q000201:enzymology; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002852:Chromatography, Ion Exchange; D003460:Crystallization; D008722:Methods; D012273:Ribulose-Bisphosphate Carboxylase / Q000302:isolation & purification*
D012273:Ribulose-Bisphosphate Carboxylase