Comparison of the in vivo inflammatory activities after intra-articular injection of natural and recombinant IL-1 alpha and IL-1 beta in the rabbit.
Comparison has been made of the in vivo pro-inflammatory activities or procine natural and human recombinant alpha and beta interleukin 1 (IL-1) after injection into the knee joints of rabbits. Both forms of pig IL-1 and human IL-1 were separately equiactive in vitro in stimulating rabbit synovial fibroblasts and articular chondrocytes to synthesize prostaglandin E2 (PGE2). Injection of IL-1 into the rabbit knee joint was not associated with swelling of the joint nor with the appearance of PGE2 in the synovial fluid. However, all preparations of IL-1 induced a dose-dependent increase in inflammatory leukocytes in the synovial lining and joint cavity. In addition, both the alpha and beta forms of IL-1 from both species caused loss of proteoglycan from the matrix of articular cartilage. This study demonstrates that both genetically distinct forms of IL-1 have the same range of inflammatory actions within the joint and that they have similar potencies in these respects.
Henderson
B
B
Department of Pharmacology, Wellcome Research Laboratories, Beckenham, Kent, U.K.
Pettipher
E R
ER
D000818:Animals; D001172:Arthritis, Rheumatoid / Q000503:physiopathology; D002358:Cartilage, Articular / Q000378:metabolism; D003238:Connective Tissue / Q000378:metabolism; D015232:Dinoprostone / Q000378:metabolism; D007249:Inflammation / Q000139:chemically induced* / Q000473:pathology / Q000503:physiopathology; D007270:Injections, Intra-Articular; D007375:Interleukin-1 / Q000008:administration & dosage / Q000494:pharmacology*; D007958:Leukocyte Count; D009000:Monocytes / Q000166:cytology; D009504:Neutrophils / Q000166:cytology; D011509:Proteoglycans / Q000378:metabolism; D011817:Rabbits; D011994:Recombinant Proteins / Q000494:pharmacology
D007375:Interleukin-1; D011509:Proteoglycans; D011994:Recombinant Proteins; D015232:Dinoprostone