Establishment of equine T-lymphocyte cultures dependent on recombinant human interleukin-2.
Long-term equine lymphocyte cultures were initiated and maintained in continuous culture with medium containing recombinant human interleukin-2. Cultures were successfully maintained with lectin activation signals and recombinant human interleukin-2 or with recombinant human interleukin-2 alone. All cell cultures that were characterized had a T-lymphocyte phenotype and had lectin-dependent or -independent cytotoxicity directed to various cell types. These findings demonstrate that long-term equine T-lymphocytes cultures can be initiated and maintained easily.
Stott
M L
ML
Department of Veterinary Pathology, University of California, Davis 95616.
Osburn
B I
BI
D000818:Animals; D002455:Cell Division; D002478:Cells, Cultured; D003602:Cytotoxicity, Immunologic; D006736:Horses / Q000097:blood* / Q000276:immunology; D007376:Interleukin-2 / Q000494:pharmacology*; D008213:Lymphocyte Activation; D010641:Phenotype; D011994:Recombinant Proteins / Q000494:pharmacology; D013601:T-Lymphocytes / Q000166:cytology* / Q000276:immunology
D007376:Interleukin-2; D011994:Recombinant Proteins