Comparison of thymocyte and T lymphocyte gangliosides from C3H/HeN and C3H/HeJ mice.
Gangliosides have been prepared from resting murine thymocytes and splenic T cells. Profoundly different two-dimensional thin layer chromatography (2D TLC) patterns were observed between these two cell types. Thymocytes contained 28-30 discrete gangliosides of which eight represented major gangliosides. Splenic T lymphocytes from both strains had much simpler patterns, with six to seven major gangliosides and 12-13 minor gangliosides. Computerized analysis of the thymocyte ganglioside patterns between LPS-responder C3H/HeN mice and lipopolysaccharide (LPS)-hyporesponsive C3H/HeJ mice revealed no significant difference in the major gangliosides. However, with splenic T cell gangliosides, there is a striking difference in the relative proportion of three homologous gangliosides between the two strains. Consistent with previous observations on macrophage gangliosides, the ratio of N-acetylneuraminic acid-containing ganglioside to N-glycolylneuraminic acid-containing ganglioside was higher in both thymocytes and T-cells from the LPS-responder strain. These results show that sialic acid-containing glycolipids from thymocytes and T lymphocytes between endotoxin responder and hyporesponder strains manifest small but significant changes. These differences are present in unstimulated cell populations and may represent a manifestation of the Lps gene.
Yohe
H C
HC
Department of Internal Medicine, Yale University School of Medicine, West Haven, CT 06516.
Cuny
C L
CL
Berenson
C S
CS
Ryan
J L
JL
D000818:Animals; D002855:Chromatography, Thin Layer; D005260:Female; D005732:Gangliosides / Q000032:analysis*; D008070:Lipopolysaccharides / Q000378:metabolism; D051379:Mice; D008809:Mice, Inbred C3H; D019158:N-Acetylneuraminic Acid; D012794:Sialic Acids / Q000032:analysis; D013045:Species Specificity; D013601:T-Lymphocytes / Q000032:analysis*
D005732:Gangliosides; D008070:Lipopolysaccharides; D012794:Sialic Acids; D019158:N-Acetylneuraminic Acid