title: Calcitonin gene-related peptide in hamster lung and its coexistence with serotonin: a chemical and immunocytochemical study.
abstract: The mammalian lung may have an important endocrine function besides being involved in gas exchange mechanisms. A number of peptide hormones have been localized to neurons and endocrine cells in the lung where they may contribute to the regulation of local pulmonary functions. We have investigated the presence of calcitonin gene-related peptide (CGRP), in the hamster lung by radioimmunoassay and by immunocytochemistry. Measurable quantities of CGRP were detected in lung tissue. Females had higher lung tissue levels of CGRP-like immunoreactivity (IR) than males. This was not reflected in an observable increase in the intensity or distribution of CGRP-like reactivity with immunocytochemistry. Distinct CGRP-like IR was recorded in clustered (NEB) and solitary (NEC) neuroendocrine cells in neonates, weanlings and adults, including all airways from trachea (NEC only) to bronchi, bronchioles, and alveolar ducts to the level of alveoli (NEC and NEB). In adult hamsters, there seemed to be fewer immunoreactive cells, although intensity was unchanged. In addition some NEB contained serotonin-like IR, and colocalization of the peptide and the amine was noted within some cells. Intra-epithelial beaded nerve fibers, subepithelial fibers, and large-caliber nerves in the hilus region and tracheal wall were also CGRP-IR, and immunoreactive nerves were occasionally found in close association with NEB at the basal pole. Positive nerve fibers were not observed in vessels within the lung, and were sparse in the adventitia of tracheal arteries.
authors.affiliation: School of Veterinary Medicine, University of Wisconsin, Madison 53706.
authors.forename: I M
authors.initials: IM
authors.lastname: Keith
authors.forename: R
authors.initials: R
authors.lastname: Ekman
mesh_terms: D000818:Animals; D015740:Calcitonin Gene-Related Peptide; D006224:Cricetinae; D005260:Female; D007150:Immunohistochemistry; D008168:Lung / Q000032:analysis*; D008297:Male; D008647:Mesocricetus; D009479:Neuropeptides / Q000032:analysis*; D012701:Serotonin / Q000032:analysis*
chemical_list: D009479:Neuropeptides; D012701:Serotonin; D015740:Calcitonin Gene-Related Peptide