title: A motor-driven syringe-type gradient maker for forming immobilized pH gradient gels.
abstract: A motor driven gradient maker based on the commercial model (Jule Inc., Trumbull, CT) was designed for immobilized pH gradient gels to provide small volumes, rapid stirring and delivery, strict volume and temperature control and air exclusion. The device was constructed and by a convenient procedure yields highly reproducible gradients either in solution or on polyacrylamide gels.
authors.affiliation: Section on Macromolecular Analysis, National Institute of Child Health and Human Development, Bethesda, MD 20892.
authors.forename: J S
authors.initials: JS
authors.lastname: Fawcett
authors.forename: J V
authors.initials: JV
authors.lastname: Sullivan
authors.forename: B E
authors.initials: BE
authors.lastname: Chidakel
authors.forename: A
authors.initials: A
authors.lastname: Chrambach
mesh_terms: D000180:Acrylic Resins; D004586:Electrophoresis / Q000295:instrumentation*; D005782:Gels; D006863:Hydrogen-Ion Concentration
chemical_list: D000180:Acrylic Resins; D005782:Gels; C016680:polyacrylamide gels