title: Utilization of medium-chain triglycerides and octanoic acid by cultured human fibroblasts and lymphoblasts.
abstract: Cultured human fibroblasts and lymphoblasts were incubated with emulsions containing 14C-trioctanoin or 14C-tripalmitin. Both cell types were able to hydrolyse the medium-chain triglyceride but not the long-chain triglyceride to the corresponding fatty acids. At the end of a 3 days incubation period, 25-30% of the initial amount of 10 nmol/ml trioctanoin were present as triglyceride. The observed hydrolysis seems to be mediated by an esterase secreted into the culture medium, as was shown by the use of cell-conditioned medium. CO2 production from octanoic acid was below 2 nmol per mg protein and day, demonstrating that these cells have a low capacity to use this substrate for their energy metabolism.
authors.affiliation: Medizinische Poliklinik, Universität München, Garching.
authors.forename: C
authors.initials: C
authors.lastname: Lemmen
authors.forename: K
authors.initials: K
authors.lastname: Stuhler
authors.forename: P
authors.initials: P
authors.lastname: Lemmen
authors.forename: G
authors.initials: G
authors.lastname: Wolfram
mesh_terms: D000818:Animals; D002210:Caprylates / Q000378:metabolism*; D002250:Carbon Radioisotopes; D002478:Cells, Cultured; D003571:Cytochalasin B / Q000494:pharmacology; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D009132:Muscles / Q000378:metabolism*; D010084:Oxidation-Reduction; D051381:Rats; D012867:Skin / Q000378:metabolism; D014280:Triglycerides / Q000378:metabolism*
chemical_list: D002210:Caprylates; D002250:Carbon Radioisotopes; D014280:Triglycerides; D003571:Cytochalasin B; C003637:tricaprylin; C005859:tripalmitin