title: Determination of ambenonium in biological samples by reversed-phase ion-pair liquid chromatography.
abstract: A sensitive and selective analytical method for the determination of ambenonium ion in biological samples is described. The procedure involves ion-pair extraction of the drug, followed by reversed-phase ion-pair chromatographic analysis with ultraviolet detection at 217 nm. The detection limits at a signal-to-noise ratio of 5 were 100 pmol/ml using 0.2 ml of plasma and bile, 250 pmol/ml using 0.2 ml of urine and 200 pmol/g using 1 ml of tissue homogenates containing 0.1 g/ml of each tissue. This assay procedure was used to study the pharmacokinetics of ambenonium ion after intravenous administration in rats.
authors.affiliation: Hospital Pharmacy, Faculty of Medicine, University of Tokyo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Yamamoto
authors.forename: Y
authors.initials: Y
authors.lastname: Kohda
authors.forename: Y
authors.initials: Y
authors.lastname: Saitoh
authors.forename: F
authors.initials: F
authors.lastname: Nakagawa
mesh_terms: D000549:Ambenonium Chloride / Q000097:blood / Q000378:metabolism* / Q000652:urine; D000818:Animals; D001646:Bile / Q000032:analysis / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D006863:Hydrogen-Ion Concentration; D008297:Male; D011759:Pyrrolidines; D051381:Rats; D011919:Rats, Inbred Strains; D012015:Reference Standards; D014018:Tissue Distribution
chemical_list: D011759:Pyrrolidines; D000549:Ambenonium Chloride; C004460:benzilonium bromide