Inhibition of human granulocyte-macrophage colony formation by interleukin 2-treated lymphocytes.
The effects of interleukin 2 (IL-2)-treated lymphocytes on human myeloid progenitors (CFU-GM) were studied. When peripheral blood mononuclear cells (PBMC) were cultured for 3 days with recombinant IL-2, they developed lymphokine-activated killer (LAK) activity against normal bone marrow cells, and also suppressed colony formation by CFU-GM. Suppression of CFU-GM was found to be mediated mainly by natural killer (NK) cells, and to a lesser degree by T cells according to the results showing that isolated NK cells and T cells exhibited strong and moderate suppressor function, respectively. Since the levels of LAK activity and of CFU-GM inhibitory activity were not parallel in each individual, inhibition of CFU-GM does not seem to be due to a direct lytic action by LAK cells. This possibility was supported by our finding that the supernatant of IL-2-treated PBMC contained factor(s) that inhibited CFU-GM colony formation.
Takahashi
M
M
Department of Medicine, Tokyo Women's Medical College, Japan.
Oshimi
K
K
Saito
H
H
Mizoguchi
H
H
D003864:Depression, Chemical; D006098:Granulocytes / Q000166:cytology*; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology*; D008214:Lymphocytes / Q000187:drug effects*; D008264:Macrophages / Q000166:cytology*; D013234:Stem Cells / Q000187:drug effects
D007376:Interleukin-2