In vitro susceptibility of canine tumor stem cells to doxorubicin.
Thirty-four canine tumor specimens (13 sarcomas and 21 carcinomas) were grown in soft agar gel. Susceptibility after continuous exposure to doxorubicin at concentrations of 1, 5, and 10 micrograms/ml was compared with that of control cultures. Plating efficiency averaged 0.096%. Doxorubicin at a concentration of 1 microgram/ml was found to result in greater than 70% inhibition of colony formation in 3 of 13 sarcomas and 1 of 21 carcinomas tested, and greater than 50%, but less than 70%, inhibition in 3 carcinomas and 1 sarcoma. Twenty-six tumors had less than 50% reduction in colony formation and were considered resistant. Minor differences in responses to drug in primary and metastatic locations were observed and attributed to tumor heterogeneity and test variability.
Macy
D W
DW
Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins 80523.
Ensley
B A
BA
Gillette
E L
EL
D000818:Animals; D002277:Carcinoma / Q000188:drug therapy / Q000662:veterinary*; D003114:Colony-Forming Units Assay; D004283:Dog Diseases / Q000188:drug therapy*; D004285:Dogs; D004317:Doxorubicin / Q000494:pharmacology / Q000627:therapeutic use*; D014411:Neoplastic Stem Cells / Q000187:drug effects; D012509:Sarcoma / Q000188:drug therapy / Q000662:veterinary*; D014407:Tumor Cells, Cultured; D014410:Tumor Stem Cell Assay
D004317:Doxorubicin