Evidence for an adenine-dependent inhibitor in serum and plasma which retards the reactivation of lens epithelial cells in primary culture.
Primary culture of epithelium-capsule-preparations (ECP) from eye lenses can be performed in a HEPES- and EDTA-buffered salt solution. Addition of serum causes an initial retraction of the epithelium, and thereafter it stimulates spreading and flattening of the cells. In the presence of 10 microM adenine the serum-evoked retraction is strengthened, and the serum-stimulated spreading is inhibited. These effects of adenine are mediated by a low molecular weight serum factor, which was partially purified.
Glaesser
D
D
Institute of Biochemistry, Martin Luther University, GDR, Halle, Saale.
Ngoli
D
D
Iwig
M
M
D000225:Adenine / Q000494:pharmacology*; D000818:Animals; D001685:Biological Factors / Q000302:isolation & purification*; D001792:Blood Platelets / Q000502:physiology; D002417:Cattle; D002478:Cells, Cultured; D004847:Epithelial Cells; D004848:Epithelium / Q000187:drug effects; D007700:Kinetics; D007908:Lens, Crystalline / Q000166:cytology* / Q000187:drug effects
D001685:Biological Factors; D000225:Adenine