title: Inhibition of cellular phosphatidylinositol turnover by psi-tectorigenin.
abstract: Psi-tectorigenin, an isoflavonoid, was isolated from a culture filtrate of actinomycetes as an inhibitor of epidermal growth factor-induced phosphatidylinositol turnover in cultured A431 cells. It inhibited phosphatidylinositol turnover with an IC50 of about 1 microgram/ml; thus, its inhibitory activity was 6-times stronger than that of genistein or orobol. When added to cultured A431 cells psi-tectorigenin inhibited phosphatidylinositol turnover without inhibiting epidermal growth factor receptor tyrosine protein kinase. Thus, psi-tectorigenin is a specific inhibitor of phosphatidylinositol turnover and may be a useful tool for the functional analysis of phosphatidylinositol turnover.
authors.affiliation: Institute of Microbial Chemistry, Tokyo, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Imoto
authors.forename: T
authors.initials: T
authors.lastname: Yamashita
authors.forename: T
authors.initials: T
authors.lastname: Sawa
authors.forename: S
authors.initials: S
authors.lastname: Kurasawa
authors.forename: H
authors.initials: H
authors.lastname: Naganawa
authors.forename: T
authors.initials: T
authors.lastname: Takeuchi
authors.forename: Z
authors.initials: Z
authors.lastname: Bao-quan
authors.forename: K
authors.initials: K
authors.lastname: Umezawa
mesh_terms: D000192:Actinomycetales / Q000378:metabolism*; D002460:Cell Line; D004815:Epidermal Growth Factor / Q000037:antagonists & inhibitors; D066246:ErbB Receptors / Q000378:metabolism; D005419:Flavonoids / Q000494:pharmacology*; D007529:Isoflavones / Q000302:isolation & purification / Q000494:pharmacology*; D010710:Phosphates / Q000378:metabolism; D010716:Phosphatidylinositols / Q000378:metabolism*; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors
chemical_list: D005419:Flavonoids; D007529:Isoflavones; D010710:Phosphates; D010716:Phosphatidylinositols; D004815:Epidermal Growth Factor; C055439:psi-tectorigenin; D066246:ErbB Receptors; D011505:Protein-Tyrosine Kinases