Improved hepatic function in the 24-hour preserved rat liver with UW-lactobionate solution and SRI 63-441.
The present study compares rat liver preservation for 9, 12, and 24 h in the standard Eurocollins solution with preservation for the same time periods in the new UW-lactobionate solution. Pharmacologic manipulation with a potent platelet-activating factor antagonist, SRI 63-441, was also evaluated. After cold storage in each of the test solutions, the livers underwent 90 min of warm, oxygenated, sanguinous perfusion. A significant increase in liver weight was noted in Eurocollins-stored versus UW-lactobionate-stored livers. After 90 min of perfusion, livers preserved in UW-lactobionate produced significantly more bile and liberated significantly less glucose and transaminases when compared with Eurocollins-stored livers. Significant augmentation of bile production was observed when donor animals were pretreated with SRI 63-441 and the livers were then stored in UW-lactobionate for 24 h. Eurocollins-stored livers demonstrated increased hepatocyte vacuolization and endothelial disruption when compared with UW-lactobionate-stored livers after 12 and 24 h of preservation. This study demonstrates the superiority of UW-lactobionate solution in liver preservation and suggests that SRI 63-441 may be beneficial in the further reduction of cold ischemic injury.
Ontell
S J
SJ
Department of Surgery, University of Pittsburgh School of Medicine, Pennsylvania.
Makowka
L
L
Ove
P
P
Starzl
T E
TE
D000241:Adenosine; D000493:Allopurinol; D000818:Animals; D005978:Glutathione; D006982:Hypertonic Solutions; D007328:Insulin; D008099:Liver / Q000502:physiology; D008297:Male; D009926:Organ Preservation / Q000379:methods*; D019188:Organ Preservation Solutions; D010972:Platelet Activating Factor / Q000037:antagonists & inhibitors*; D011806:Quinolinium Compounds; D011887:Raffinose; D051381:Rats; D011917:Rats, Inbred Lew; D012996:Solutions; D013997:Time Factors
C037096:Euro-Collins' solution; D006982:Hypertonic Solutions; D007328:Insulin; D019188:Organ Preservation Solutions; D010972:Platelet Activating Factor; D011806:Quinolinium Compounds; D012996:Solutions; C055206:University of Wisconsin-lactobionate solution; C050531:SRI 63-441; D000493:Allopurinol; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose