Production of a fibronectin-associated lymphokine by cloned mouse T cells.
Azobenzenearsonate-specific cloned mouse T cells able to transfer delayed hypersensitivity reactions in vivo produced macrophage agglutination factor (MaggF) after stimulation with mitogen or antigen in vitro. Mitogen (Con A) elicited MAggF production directly from T cells. Responses to Ag were Ag-specific, required syngeneic accessory cells in addition to T cells, and were independent of T cell fine specificity for azobenzenearsonate. Mouse MAggF shared a number of biochemical and immunochemical properties with the fibronectins (FN): 1) high Mr similar to that of plasma FN; 2) binding to gelatin, heparin, and polyclonal antibodies and mAb specific for cellular and plasma FN; 3) inhibition of activity in solution by monoclonal anti-human FN directed against plasma FN gelatin-binding domain; and 4) action on peritoneal exudate macrophages mediated through a FN-receptor cross reactive with one on human monocytes. MAggF production required active protein synthesis and was associated with significant increases in gelatin-binding immunoreactive FN (Mr 440 kDa on immunoblotting) in culture supernatants and T cell lysates. Metabolically labeled peptides could be precipitated by anti-FN from culture supernatants of activated T cells. Stimulated cultures contained significantly more cells with immunohistologically demonstrable cytoplasmic FN than unstimulated control cultures. We suggest that T cell FN is a distinct species of cellular FN which may play an important role in mediating delayed hypersensitivity inflammatory reactions in vivo.
Godfrey
H P
HP
Department of Pathology, New York Medical College, Valhalla 10595.
Canfield
L S
LS
Kindler
H L
HL
Angadi
C V
CV
Tomasek
J J
JJ
Goodman
J W
JW
D000818:Animals; D002458:Cell Fractionation; D002474:Cell-Free System; D002478:Cells, Cultured; D002999:Clone Cells / Q000378:metabolism; D005353:Fibronectins / Q000032:analysis / Q000276:immunology / Q000502:physiology*; D005780:Gelatin / Q000378:metabolism; D006241:Haptens / Q000276:immunology; D006493:Heparin / Q000378:metabolism; D007150:Immunohistochemistry; D008222:Lymphokines / Q000096:biosynthesis* / Q000302:isolation & purification / Q000378:metabolism; D051379:Mice; D013601:T-Lymphocytes / Q000378:metabolism*
D005353:Fibronectins; D006241:Haptens; D008222:Lymphokines; C035408:macrophage aggregation factor; D005780:Gelatin; D006493:Heparin