Acquisition and maturation of expressed B cell repertoires in normal and autoimmune mice.
The expressed B cell repertoires of mice from 1 day to 5 mo of age were examined. The ELISA-spot assay was used to enumerate individual Ig-secreting splenic B cells and determine the proportion of these cells producing antibodies reactive with each of six autoantigens and two conventional Ag. Results indicate that i) neonatal B cells producing antibodies against specific members of the Ag panel arose in a temporally defined sequence, ii) antibodies produced by 1- to 5-wk-old mice appeared to be more cross-reactive than those produced by adult mice, iii) no bias toward autoantibody production was found in the neonatal repertoires of autoimmune-prone mice, and iv) as a whole, the pattern of repertoire development among diverse strains of mice was highly conserved, although individual mice varied considerably in the absolute number of B cells committed to the production of antibodies of a given specificity.
Klinman
D M
DM
Cellular Immunology Section, National Institute of Arthritis, Bethesda, MD 20892.
Ishigatsubo
Y
Y
Steinberg
A D
AD
D000375:Aging; D000818:Animals; D000831:Animals, Newborn / Q000254:growth & development / Q000276:immunology; D001323:Autoantibodies / Q000096:biosynthesis; D001327:Autoimmune Diseases / Q000235:genetics / Q000276:immunology* / Q000503:physiopathology; D001402:B-Lymphocytes / Q000276:immunology* / Q000378:metabolism / Q000502:physiology; D002454:Cell Differentiation; D005260:Female; D007958:Leukocyte Count; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008811:Mice, Inbred DBA; D008814:Mice, Inbred NZB; D013045:Species Specificity
D001323:Autoantibodies