Newly synthesized proteins in a cell-free system supplemented with human and bovine membrane-bound polysomes isolated from lens fiber cells.
Since there is no report on human lens membrane-bound polysomes, we analyzed the products synthesized de nove in a cell-free system under the direction of membrane-bound polysomes isolated from human and bovine lens fiber cells by one- and two-dimensional gel electrophoresis. The translation products appeared to be mainly water-insoluble polypeptides.
Ozaki
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Department of Biochemistry, University of Nijmegen, Netherlands.
D000293:Adolescent; D001345:Autoradiography; D002474:Cell-Free System; D002648:Child; D002675:Child, Preschool; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D007223:Infant; D007908:Lens, Crystalline / Q000378:metabolism*; D008566:Membranes / Q000032:analysis; D011132:Polyribosomes / Q000378:metabolism*; D014176:Protein Biosynthesis