Establishment of a peripheral T-cell lymphoma cell line showing amplification of the c-myc oncogene.
A new T-cell lymphoma cell line, designated T34, was established from freshly isolated lymph node tumor cells of a patient with non-Hodgkin's diffuse large cell lymphoma. The T34 cells, as well as the parental lymphoma cells, showed mature helper/inducer immunophenotypes in that they formed spontaneous sheep erythrocyte rosettes and reacted with OKT-3 and OKT-4 monoclonal antibodies. They were negative for OKT-6, OKT-8, terminal deoxynucleotidyl transferase, WT-1, and HLA-DR antigens. Molecular analysis revealed that the T34 cells contained 8- to 16-fold amplified c-myc DNA. The same genetic change was observed in parental lymphoma cells, indicating that the c-myc amplification had occurred in vivo. There was no gross rearrangement of the c-myc DNA. The c-myc gene of the T34 cell line was actively transcribed into normal-sized c-myc mRNA. Cytogenetic analysis showed that both the T34 and the parental lymphoma cells had a near-triploid karyotype with multiple structural chromosome changes. The terminal end of the long arm of chromosome No. 8, the chromosomal locus of single-copy c-myc, was elongated (8q+ chromosome), perhaps reflecting the site of c-myc amplification. These data suggested that amplification of the c-myc oncogene played some role in progression and proliferation of this peripheral T-cell neoplasm.
Ohno
H
H
Department of Internal Medicine, Faculty of Medicine, Kyoto University, Japan.
Fukuhara
S
S
Arita
Y
Y
Doi
S
S
Takahashi
R
R
Fujii
H
H
Honjo
T
T
Sugiyama
T
T
Uchino
H
H
D000368:Aged; D002454:Cell Differentiation; D002869:Chromosome Aberrations; D004247:DNA / Q000032:analysis; D005784:Gene Amplification; D006801:Humans; D008223:Lymphoma / Q000235:genetics* / Q000473:pathology; D008297:Male; D011519:Proto-Oncogenes; D013601:T-Lymphocytes; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
D004247:DNA