Toward an objective classification of cells in the immune system.
The relative abundance of individual proteins shared among clones of lymphocytes provides a meaningful basis for cellular classification. Twelve clones of T cells (obtained by limiting dilution) were analyzed by two-dimensional gel electrophoresis for polypeptide content and then evaluated by the computational technique known as principal component analysis. As a result, relatedness of the clones was established and expressed in terms of taxonomic distances. The data show that a comprehensive and objective classification of the cells involved in the immune system can be approached.
Lefkovits
I
I
Basel Institute for Immunology, Switzerland.
Kuhn
L
L
Valiron
O
O
Merle
A
A
Kettman
J
J
D000818:Animals; D002478:Cells, Cultured; D002999:Clone Cells; D046508:Culture Techniques / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D051379:Mice; D008810:Mice, Inbred C57BL; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000145:classification* / Q000166:cytology / Q000276:immunology