Antioxidant and free radical-scavenging enzymes in chronically ethanol-consuming rats: controversy over hepatic lipid peroxidation.
In rats given ethanol 20% (v/v) in drinking water the hepatic antioxidant enzymes, superoxide dismutase (SOD), catalase and glutathione peroxidase were assayed at the end of 4 and 10 months of ethanol consumption. Simultaneously hepatic lipid peroxidation was monitored. At 4 months SOD and catalase were unaffected, while glutathione peroxidase was decreased by 48%. By the end of 10 months SOD had declined by 16% and glutathione peroxidase activity increased by 49%, while catalase again remained stable. However, hepatic lipid peroxidation was not significantly affected throughout the study. The controversy in the literature over the conflicting results of hepatic lipid peroxidation in chronically ethanol-fed rats is discussed in the light of mode, dose, duration of ethanol consumption, nutritional status of the rat, and primacy of glutathione peroxidase.
Nadkarni
G D
GD
Radiation Medicine Centre, Bio-Medical Group, Bombay, India.
D'Souza
N B
NB
D000437:Alcoholism / Q000201:enzymology*; D000818:Animals; D002374:Catalase / Q000378:metabolism*; D005609:Free Radicals; D005979:Glutathione Peroxidase / Q000378:metabolism*; D015227:Lipid Peroxidation; D008099:Liver / Q000201:enzymology*; D008108:Liver Diseases, Alcoholic / Q000201:enzymology; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013482:Superoxide Dismutase / Q000378:metabolism*
D005609:Free Radicals; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase