The response of human marrow colony-forming units-granulocyte and macrophage to inhibition by prostaglandin E and acidic isoferritins is associated with expression of MHC class II antigens and requires the participation of a CD8+ T lymphokine.
The in vitro expression of MHC class II Ag by human bone marrow colony-forming unit-granulocyte and macrophage (CFU-GM) and their proliferative response to negative growth regulators in agar culture are transient and can be modulated in vitro by 24-h suspension culture in the presence of PGE. Analysis of the participation of accessory cells in this phenomenon indicates that the ability of PGE to modulate CFU-GM MHC class II expression, the proportion of CFU-GM in S-phase of the cell cycle and the responsiveness of CFU-GM to inhibition in vitro by two negative growth regulators, acidic isoferritin inhibitory activity and PGE itself, requires the participation of CD8+ T lymphocytes. This effect is mediated by a lymphokine of m.w. 27,000 that we have purified to apparent homogeneity. This lymphokine possesses neither colony stimulatory nor inhibitory activity, is produced by both peripheral blood and bone marrow CD8+ T lymphocytes, as well as the CEM T-ALL cell line, and requires the obligatory presence of PGE for activity.
Pelus
L M
LM
Department of Hematopoietic Regulation, Sloan Kettering Institute, New York 10021.
Levi
E
E
Welte
K
K
D000328:Adult; D000938:Antigen-Presenting Cells / Q000145:classification / Q000502:physiology; D001854:Bone Marrow Cells; D002453:Cell Cycle / Q000187:drug effects; D002469:Cell Separation; D003470:Culture Media / Q000032:analysis; D005293:Ferritins / Q000494:pharmacology*; D006098:Granulocytes / Q000187:drug effects; D006131:Growth Inhibitors / Q000494:pharmacology*; D006681:HLA-D Antigens / Q000276:immunology*; D006412:Hematopoietic Stem Cells / Q000187:drug effects*; D006801:Humans; D008264:Macrophages / Q000187:drug effects; D010641:Phenotype; D011458:Prostaglandins E / Q000494:pharmacology*; D013491:Suppressor Factors, Immunologic / Q000302:isolation & purification / Q000502:physiology*; D013601:T-Lymphocytes / Q000145:classification* / Q000502:physiology
D003470:Culture Media; D006131:Growth Inhibitors; D006681:HLA-D Antigens; D011458:Prostaglandins E; D013491:Suppressor Factors, Immunologic; D005293:Ferritins