Enhancing the sensitivity of DNA detection and recovery from agarose gels.
By inserting nitrocellulose strips into agarose gels alongside the electrophoresed lanes and passing an electric current perpendicularly in the direction of the strips, highly efficient transfer of DNA bands onto the membrane in the form of concentrated dots is achieved. DNA detection limits by this technique are enhanced, at least three times as visualized by ethidium bromide fluorescence and at least twice more by radiolabeling.
Georges
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Plant Biotechnology Institute, National Research Council of Canada, Saskatoon, Saskatchewan.
Girard
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Chibbar
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Constabel
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D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D015180:Electrophoresis, Gel, Two-Dimensional; D009693:Nucleic Acid Hybridization; D010957:Plasmids
D004247:DNA