DNA synthesis and mitotic division of cortical neurons in adult rats upon intrabrain transplantation of embryonic nervous tissue.
The possibility of DNA synthesis and mitotic division of brain neurons in mammals was experimentally studied. Brain cortex tissue of 17- and 20-day-old rat embryos was transplanted into the sensomotor region of the right brain hemisphere of adult Wistar rats weighing 160-200 g, intact and subjected to hypoxia. 3 days after operation part of animals was injected intraperitoneally with 3H-thymidine at a dose of 10 microCi/g b.w. and killed after 4 days. Brain tissue containing the transplant was used for preparing histological sections and the surrounding cortex tissue was used for obtaining squashed preparations. Preparations of the both experimental series were treated autoradiographically and stained with cresyl-videt. It has been established that in the both experimental series some recipient cortical neurons surrounding the transplant intensively incorporate 3H-thymidine in their nuclei (i.e. replicatively synthesize DNA) and a number of nerve cells are in the state of mitotic division. An electron-microscopic study has confirmed that part of mitotically dividing cells have characteristics of neurons: oval bodies without pseudopodia electron-dense cytoplasm with developed organelles and RNP-particles typical of neurons, axonal terminals on the body, and satellitary glia.
Polezhaev
L V
LV
N. I. Vavilov Institute of General Genetics, USSR Academy of Sciences.
Alexandrova
M A
MA
Kleschhinov
V N
VN
D000818:Animals; D001345:Autoradiography; D002540:Cerebral Cortex / Q000473:pathology / Q000637:transplantation*; D004261:DNA Replication; D004622:Embryo, Mammalian; D002534:Hypoxia, Brain / Q000473:pathology; D008854:Microscopy, Electron; D008938:Mitosis; D009410:Nerve Degeneration; D009416:Nerve Regeneration; D009473:Neuronal Plasticity; D009474:Neurons / Q000473:pathology; D051381:Rats