Purification and partial sequence of rabbit polymorphonuclear leukocyte-derived lymphocyte proliferation potentiating factor resembling IL-1 beta.
A rabbit polymorphonuclear leukocytes (PMN)-derived lymphocyte proliferation-potentiating factor (PMN factor) was finally purified to homogeneity. PMN factor was released from early inflammatory peritoneal exudate cells (98% of PMN) stimulated with kaolin under roller bottle culture conditions. PMN factor was purified by large sequential scale steps, using membrane-type ion exchangers and gel filtration, followed in this order by HPLC steps with cationic ion exchangers and a hydroxylapatite column. Homogeneity was manifested based on the criteria of a single m.w. 18, 500 band on silver-stained polyacrylamide gel, a superimposable activity on a UV absorbance peak in analytic HPLC gel filtration, and detection of a single amino-terminal sequence. The homogeneous PMN factor had an isoelectric value of 7.2 and an activity of 1.9 x 10(7) U/mg in the thymocyte comitogenic assay. PMN factor stimulated one-half of the maximal response of thymocyte proliferation at 2.8 x 10(-12) M. Because of similarities in the physicochemical properties, specific activity, and amino-terminal sequence between rabbit PMN factor and human IL-1 beta, this PMN factor is therefore considered to be a rabbit IL-1 beta.
Goto
F
F
Department of Pathology, Kumamoto University Medical School, Japan.
Goto
K
K
Ohkawara
S
S
Kitamura
M
M
Mori
S
S
Takahashi
H
H
Sengoku
Y
Y
Yoshinaga
M
M
D000595:Amino Acid Sequence; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D006801:Humans; D007375:Interleukin-1 / Q000235:genetics; D007526:Isoelectric Point; D008213:Lymphocyte Activation / Q000187:drug effects; D008222:Lymphokines / Q000235:genetics / Q000302:isolation & purification* / Q000494:pharmacology; D051379:Mice; D008970:Molecular Weight; D009504:Neutrophils / Q000032:analysis*; D011817:Rabbits; D012689:Sequence Homology, Nucleic Acid; D013601:T-Lymphocytes / Q000187:drug effects
D007375:Interleukin-1; D008222:Lymphokines; C043695:lymphocyte proliferation potentiating factors