Effects of dithiothreitol and ascorbate on the penetration of diclofenac across excised rat dorsal skin.
The penetration of diclofenac through excised rat dorsal skin was found to be poor. Treatment of the skin with a reductant such as dithiothreitol or ascorbate increased the permeability of the skin to diclofenac, along with an increase in the protein thiol content of the stripped skin surface tissue. An increase in protein thiol content may increase the hydration capability of the stratum corneum.
Nishihata
T
T
Pharmaceutical Chemistry Department, University of Kansas, Lawrence 66046.
Rytting
J H
JH
Takahashi
K
K
Sakai
K
K
D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology*; D004008:Diclofenac / Q000493:pharmacokinetics*; D004229:Dithiothreitol / Q000494:pharmacology*; D066298:In Vitro Techniques; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012869:Skin Absorption / Q000187:drug effects*
D004008:Diclofenac; D001205:Ascorbic Acid; D004229:Dithiothreitol