Inhibitory effect of homologous solubilized zona pellucida on rabbit in vitro fertilization.
Rabbit zonae pellucidae were isolated using a modified technique of Dunbar et al. (1980). Zonae pellucidae were solubilized in saline phosphate buffer (pH 7.8) for 30 min at 70 degrees C. One dimensional SDS-PAGE analysis showed that zona pellucida is essentially composed of three major proteins with apparent molecular weights of 200 (275-165) kd, 100 (135-96) kd and 75 (96-51) kd. Preincubation of sperm with heat-solubilized zonae pellucidae (SZP) (5 to 8 SZP/microliters) did not reduce sperm binding ability. By contrast, it significantly decreased the percentage of penetrated eggs (20 versus 73% for the control) and significantly reduced the fertilization rate (10 versus 55% for the control). Ultrastructural analysis of several oocytes in the two groups demonstrated that inhibition of fertilization was not due to the inhibition of sperm-zona binding ability but essentially resulted from the impairment of sperm penetration through the zona pellucida.
Dumont
M
M
Station de Physiologie animale, I.N.R.A., Jouy-en-Josas, France.
Crozet
N
N
D000177:Acrosome / Q000502:physiology; D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005307:Fertilization in Vitro; D008297:Male; D008854:Microscopy, Electron; D008970:Molecular Weight; D009865:Oocytes / Q000648:ultrastructure; D010063:Ovum / Q000502:physiology*; D011506:Proteins / Q000032:analysis; D011817:Rabbits; D012995:Solubility; D013075:Sperm Capacitation; D013084:Sperm-Ovum Interactions; D013094:Spermatozoa / Q000502:physiology*; D015044:Zona Pellucida / Q000032:analysis / Q000502:physiology*
D011506:Proteins