Production of interleukin-3 from a T-cell neoplasm.
We have established a new T-cell line (CL-8313) that produces interleukin-3 from the spleen cells of mice with a radiation-induced myeloproliferative disorder. IL-3 activity was detected at an extremely high level in the culture medium of the CL-8313 cell line in the absence of any exogenous stimulator. A large amount of IL-3 transcript also was demonstrated in CL-8313 cells. BPA- and CSF-activity was detected at a high level in the culture medium of the CL-8313 cell line. Southern blot analysis of high molecular weight DNA from the CL-8313 cells showed they had unique rearrangement of the antigen receptor beta chain gene. Therefore, we concluded that CL-8313 cells belonged to T-lineage lymphocytes and constitutively produced a high level of IL-3.
Yoshida
K
K
Division of Physiology and Pathology, National Institute of Radiological Sciences, Chiba, Japan.
Seki
M
M
Maeda
M
M
Fujita
J
J
Tadokoro
K
K
D000818:Animals; D002460:Cell Line; D003115:Colony-Stimulating Factors / Q000096:biosynthesis; D007377:Interleukin-3 / Q000096:biosynthesis* / Q000235:genetics; D015458:Leukemia, T-Cell / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008809:Mice, Inbred C3H
D003115:Colony-Stimulating Factors; D007377:Interleukin-3