title: Enhancement of antibody responses by IgD-binding factors induced by anti-IgD treatment of spleen cells.
abstract: Treatment of normal BALB/c splenocytes with anti-mouse IgD antibodies at 0 degrees C followed by incubation of the cells at 37 degrees C resulted in the formation of soluble factors that selectively inhibit rosette formation of lymphocytes bearing Fc delta receptors with IgD-coated erythrocytes (i.e., IgD-binding factors). Treatment of the same cells with anti-IgM antibodies failed to induce the formation of the factors. Analysis of cellular mechanisms indicated that polymerized surface IgD on B cells, as well as surface IgD-anti-IgD complexes shed from the B cells, induced T cells bearing Fc delta receptors to form IgD-binding factors. The factors formed by the anti-IgD treatment of splenic lymphocytes are composed of molecular mass species of 70 and 34 kDa, as estimated by gel filtration. Both the 70- and 34-kDa IgD-binding factors enhanced IgM and IgG1 plaque-forming cell responses of sheep erythrocyte-primed mouse spleen cells to the antigen.
authors.affiliation: Subdepartment of Immunology, Johns Hopkins University School of Medicine, Good Samaritan Hospital, Baltimore, MD 21239.
authors.forename: M
authors.initials: M
authors.lastname: Adachi
authors.forename: K
authors.initials: K
authors.lastname: Ishizaka
mesh_terms: D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D000917:Antibody Formation; D002478:Cells, Cultured; D007072:Immunoglobulin D / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology*; D008222:Lymphokines / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D029584:Prostatic Secretory Proteins; D012397:Rosette Formation; D013154:Spleen / Q000276:immunology; D013491:Suppressor Factors, Immunologic / Q000276:immunology*
chemical_list: D000888:Antibodies, Anti-Idiotypic; D007072:Immunoglobulin D; D008222:Lymphokines; D029584:Prostatic Secretory Proteins; D013491:Suppressor Factors, Immunologic; C043076:beta-microseminoprotein; C013059:immunoglobulin-binding factors