Effect of estradiol on interleukin 1 synthesis by macrophages.
The effect of estradiol on interleukin 1 (IL-1) synthesis/secretion by rat peritoneal macrophages was investigated. Peritoneal adherent cells (PAC) from adult female rats secreted greater amounts of IL-1 spontaneously than those from age-matched male rats or prepubescent female rats. Ovariectomy led to reduced synthesis of IL-1 by PAC but estradiol replacement therapy of such rats effectively increased IL-1 synthesis. IL-1 secretion was also stimulated when PAC from male rats was incubated with estradiol. A combination of estradiol and LPS in vitro enhanced secretion of IL-1 by PAC even more than estradiol alone. These data provide new evidence suggesting that estradiol may play an important role in regulating synthesis of IL-1 by macrophages.
Hu
S K
SK
Inflammation and Immunology Research Department, American Cyanamid Company, Lederle Laboratories, Pearl River, NY 10965.
Mitcho
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YL
Rath
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NC
D000375:Aging / Q000378:metabolism; D000818:Animals; D002478:Cells, Cultured; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D007375:Interleukin-1 / Q000096:biosynthesis*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D010052:Ovariectomy; D051381:Rats; D011917:Rats, Inbred Lew; D012737:Sex Factors
D007375:Interleukin-1; D008070:Lipopolysaccharides; D004958:Estradiol