Interleukin-2-dependent T lymphocytes for the diagnosis and investigation of inherited metabolic disorders.
Interleukin-2-dependent T cell lines can be started from as little as 1 ml of peripheral blood and expanded to 50 x 10(6) cells within 12 days. These cells represent an easily obtainable source of nucleated tissue with certain advantages over skin fibroblasts or transformed B lymphoblastoid cells for the rapid diagnosis and study of inherited metabolic disorders. A panel of 19 enzymes were assayed in IL-2-dependent T lymphocytes and the diagnosis of four enzyme deficiencies is demonstrated using T lymphocytes.
Adolph
K
K
Department of Pediatrics, Faculty of Medicine, University of Calgary, Alberta, Canada.
Kucey
M T
MT
Hodges
S D
SD
Carter
R J
RJ
Snyder
F F
FF
D002478:Cells, Cultured; D006801:Humans; D007376:Interleukin-2 / Q000502:physiology*; D008661:Metabolism, Inborn Errors / Q000175:diagnosis* / Q000201:enzymology; D013601:T-Lymphocytes / Q000201:enzymology / Q000502:physiology*
D007376:Interleukin-2