title: Abnormal expression of a late gene family L1 protein in monkey cells abortively infected with adenovirus type 2.
abstract: The drastically reduced virus yields obtained from monkey cells abortively infected with adenovirus 2 (Ad2) have been attributed primarily to a severe decrease in the accumulation of the virion protein fiber (IV), a product of the most pomoter distal late gene family, L5. Here we report that the accumulation of virion protein IIIa, a product of the proximal late gene family, L1, is also severely depressed. In contrast, the i-leader protein LO-13.6K and L1 protein(s) 52K/55K are expressed with the same time course and in equal amounts in monkey cells abortively infected by Ad2 or productively infected by the Ad2-simian virus 40 (SV40) hybrid Ad2+ND1 or by the host range mutant Ad2+ND3 hr602. L1-52K/55K is phosphorylated in abortively infected CV-1 or CV-C monkey cells as well as in productively infected human and monkey cells. As with fiber expression, the failure to produce IIIa appears to be due partly to reduced or delayed IIIa mRNA accumulation. The small amount of IIIa protein that is synthesized in monkey cells is stable. Since the accumulation of both IIIa and fiber protein is deficient, the mechanism of abortive infection cannot be attributed solely to the absence of the auxiliary fiber leader sequences (1).
authors.affiliation: Biology Department, Brookhaven National Laboratory, Upton, NY 11973.
authors.forename: C W
authors.initials: CW
authors.lastname: Anderson
authors.forename: M M
authors.initials: MM
authors.lastname: Hardy
authors.forename: J B
authors.initials: JB
authors.lastname: Lewis
mesh_terms: D000260:Adenoviruses, Human / Q000235:genetics* / Q000254:growth & development; D000818:Animals; D015153:Blotting, Western; D036022:Capsid Proteins; D002460:Cell Line; D006801:Humans; D008970:Molecular Weight; D005810:Multigene Family; D010766:Phosphorylation; D012333:RNA, Messenger / Q000096:biosynthesis; D012367:RNA, Viral / Q000096:biosynthesis; D014764:Viral Proteins / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D036022:Capsid Proteins; D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins; C052227:protein IIIa, Human adenovirus type 2