Superoxide dismutase activity during dimethylhydrazine colon carcinogenesis and the effects of cholic acid and indole.
Copper, zinc superoxide dismutase (Cu,ZnSOD) and manganese superoxide dismutase (MnSOD) activities were measured in mouse large intestinal mucosa during dimethylhydrazine (DMH) carcinogenesis. Mice were divided into five groups. Group A was subcutaneously injected with DMH (20 mg/kg) weekly and fed with a diet containing 0.2% cholic acid (C) and 0.8% indole (I). Group B was injected with DMH and given indole feeding. Group C was treated with DMH injection and cholic acid feeding. Group D was given DMH injection alone. Group E was an age-matched control group given 0.9% NaCl injection. The experiment last 21 weeks. The Cu, ZnSOD activity of intestinal mucosa in group A animals began to increase significantly at the 7th week of the experiment. In groups B, C and D, however, this enzyme was not elevated statistically until the 16th week, and then each of these groups kept an increased Cu,ZnSOD level the rest of the experimental period. MnSOD activity was elevated statistically in group C animals at the 7th week. The enzyme activity in group A and D animals increased at the 9th week, but the enzyme activity did not increase statistically until the 11th week in group B. After the 16th week of the experiment the increased activity of MnSOD in all experimental groups returned to the level of the control group. Large intestinal cancer tissues had increased Cu,ZnSOD activity and decreased MnSOD activity.
Sun
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Y
Department of Biochemistry, Zhejiang Medical University, Hangchou, The People's Republic of China.
Li
Y
Y
Oberley
L W
LW
D000230:Adenocarcinoma / Q000139:chemically induced*; D000818:Animals; D019826:Cholic Acid; D002793:Cholic Acids / Q000494:pharmacology; D003110:Colonic Neoplasms / Q000139:chemically induced*; D004127:Dimethylhydrazines; D007211:Indoles / Q000494:pharmacology; D051379:Mice; D013482:Superoxide Dismutase / Q000378:metabolism*; D013997:Time Factors
D002793:Cholic Acids; D004127:Dimethylhydrazines; D007211:Indoles; C030374:indole; D013482:Superoxide Dismutase; D019826:Cholic Acid