Analysis of the suppression of IL-1 alpha and IL-1 beta production in human peripheral blood mononuclear adherent cells by a glucocorticoid hormone.
The suppression of IL-1 production in human adherent PBMC by a glucocorticoid hormone was studied. From 10(-5) to 10(-8) M glucocorticoid hormone (prednisolone) inhibited both IL-1 alpha and IL-1 beta production by LPS-stimulated adherent human PBMC in a dose-dependent fashion, as assayed by Western blotting of cell-associated IL-1 and a thymocyte comitogenic bioassay. IL-1 alpha and -beta mRNA levels were concomitantly suppressed by glucocorticoid treatment, as determined by Northern blotting analysis. These data suggest that the suppression of IL-1 activity by glucocorticoids is consistently associated with decreased levels of IL-1 proteins and mRNA expression and therefore presumably regulated at the transcriptional level.
Lew
W
W
Laboratory of Molecular Immunoregulation, National Cancer Institute, Frederick, MD 21701.
Oppenheim
J J
JJ
Matsushima
K
K
D003864:Depression, Chemical; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D007375:Interleukin-1 / Q000096:biosynthesis*; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects; D011239:Prednisolone / Q000494:pharmacology*; D011498:Protein Precursors / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D014158:Transcription, Genetic / Q000187:drug effects
D007375:Interleukin-1; D008070:Lipopolysaccharides; D011498:Protein Precursors; D012333:RNA, Messenger; D011239:Prednisolone