Regulation of BSF-2/IL-6 production by human mononuclear cells. Macrophage-dependent synthesis of BSF-2/IL-6 by T cells.
Regulation of BSF-2/IL-6 production in peripheral mononuclear cells (MNC) was studied. BSF-2 mRNA expression in mitogen-stimulated MNC showed a biphasic response, the first peak around 4 h and the second peak around 48 h. This was caused by different kinetics of BSF-2 mRNA expression in distinct subpopulations of MNC; M phi expressed BSF-2 mRNA at 5 h in the absence of any stimulation, and mitogen-stimulated T cells and B cells expressed BSF-2 mRNA 48 h after stimulation. Immunohistochemical staining of the cells with anti-BSF-2 antibody demonstrated that macrophages, T cells and B cells could produce BSF-2. T cells in peripheral MNC produced BSF-2 in the presence of M phi. The requirement of macrophage for BSF-2 production in T cells could be replaced by TPA but not by IL-1 or BSF-2.
Horii
Y
Y
Division of Immunology, School of Medicine, Osaka University, Japan.
Muraguchi
A
A
Suematsu
S
S
Matsuda
T
T
Yoshizaki
K
K
Hirano
T
T
Kishimoto
T
T
D001402:B-Lymphocytes / Q000032:analysis / Q000276:immunology / Q000378:metabolism; D002478:Cells, Cultured; D002648:Child; D006801:Humans; D007118:Immunoassay; D007150:Immunohistochemistry; D015850:Interleukin-6; D007378:Interleukins / Q000096:biosynthesis* / Q000302:isolation & purification; D007700:Kinetics; D008213:Lymphocyte Activation; D008264:Macrophages / Q000276:immunology* / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000378:metabolism; D013601:T-Lymphocytes / Q000032:analysis / Q000276:immunology / Q000378:metabolism*
D015850:Interleukin-6; D007378:Interleukins; D012333:RNA, Messenger