The use of cell permeabilization for studying the mechanisms of action of angiotensin II in the adrenal cortex and vascular smooth muscle.
Some applications of the technique of electropermeabilization to the study of the mechanisms of action of angiotensin II in two of its physiological target cells, the adrenal zona glomerulosa and the vascular smooth muscle cell, are described. The technique proved useful for characterizing the second messenger role of inositol 1,4,5-trisphosphate, its metabolic pathways and the effect of Ca2+ on steroidogenesis.
Capponi
A M
AM
Division of Endocrinology, University Hospital, Geneva, Switzerland.
Rossier
M F
MF
Vallotton
M B
MB
D000302:Adrenal Cortex / Q000187:drug effects* / Q000378:metabolism; D000450:Aldosterone / Q000096:biosynthesis; D000804:Angiotensin II / Q000494:pharmacology*; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002417:Cattle; D002463:Cell Membrane Permeability; D015544:Inositol 1,4,5-Trisphosphate; D007295:Inositol Phosphates / Q000378:metabolism; D009131:Muscle, Smooth, Vascular / Q000187:drug effects* / Q000378:metabolism; D051381:Rats
D007295:Inositol Phosphates; D000804:Angiotensin II; D000450:Aldosterone; D015544:Inositol 1,4,5-Trisphosphate; D002118:Calcium