title: [Quantitative evaluation of serum IgG subclass levels by an immunoenzyme method].
abstract: We developed a enzyme linked immunosorbent assay (ELISA) for measuring IgG subclasses concentration in serum. For this we used monoclonal antibodies. The specificity of these antibodies was evaluated with a panel of myeloma proteins belonging to the 4 IgG subclasses. The ELISA was sensitive (allowing the detection of subclasses at ng level) and accurate (inter-assay coefficient of variation of 14%). Using the WHO serum 67/97 as reference, we determined the concentration of IgG subclasses in a pool of sera. In addition concentrations were measured in 69 healthy adults to study the distribution of each IgG subclass. A good correlation (r = 0.78) was obtained between the sum of the subclasses measured by ELISA and total IgG measured by immunonephelometry.
authors.affiliation: Laboratoire d'Immunochimie, Institut National de Transfusion Sanguine, Paris, France.
authors.forename: P
authors.initials: P
authors.lastname: Lambin
authors.forename: M
authors.initials: M
authors.lastname: Dubarry
authors.forename: C
authors.initials: C
authors.lastname: Charron
authors.forename: N
authors.initials: N
authors.lastname: Herance
authors.forename: E
authors.initials: E
authors.lastname: Defendini
mesh_terms: D000328:Adult; D000918:Antibody Specificity; D004797:Enzyme-Linked Immunosorbent Assay / Q000592:standards; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis / Q000145:classification* / Q000592:standards; D008297:Male; D012015:Reference Standards; D012016:Reference Values
chemical_list: D007074:Immunoglobulin G
vernacular_title: Estimation quantitative du taux des sous-classes d'IgG sériques par une méthode immunoenzymatique.