title: The use of in vivo brain dialysis of dopamine, acetylcholine, amino acids and lactic acid in studies on the neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP).
abstract: The use of intracerebral brain dialysis in freely moving rats in neurochemical and neurotoxicological research is discussed and exemplified by studies on the neurotoxin MPTP. Intrastriatal administration of its toxic metabolite MPP+, via the dialysis tube, induced massive changes in the release of neurotransmitters and metabolites. Release enhancing effects could not be repeated by a second MPP+ perfusion and decreases in neurotransmitter or metabolite output were persistent. This indicates that MPP+ has irreversible, toxic effects on various neuortransmitter systems. The MPP+-induced release of DA has been characterized by studying the effect of pretreatment with various drugs, as well by comparison of the time courses of MPP+-induced DA release with those of amphetamine-induced DA release and of MPP+-induced lactate overflow.
authors.affiliation: Department of Medicinal Chemistry, State University Groningen, The Netherlands.
authors.forename: H
authors.initials: H
authors.lastname: Rollema
authors.forename: J B
authors.initials: JB
authors.lastname: de Vries
authors.forename: G
authors.initials: G
authors.lastname: Damsma
authors.forename: B H
authors.initials: BH
authors.lastname: Westerink
authors.forename: G L
authors.initials: GL
authors.lastname: Kranenborg
authors.forename: W G
authors.initials: WG
authors.lastname: Kuhr
authors.forename: A S
authors.initials: AS
authors.lastname: Horn
mesh_terms: D015632:1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine; D015655:1-Methyl-4-phenylpyridinium; D019297:2,4-Dinitrophenol; D000109:Acetylcholine / Q000378:metabolism*; D000596:Amino Acids / Q000378:metabolism*; D000661:Amphetamine / Q000494:pharmacology; D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D003956:Dialysis; D004140:Dinitrophenols / Q000494:pharmacology; D004298:Dopamine / Q000378:metabolism*; D007773:Lactates / Q000378:metabolism*; D019344:Lactic Acid; D011725:Pyridines / Q000633:toxicity*; D011726:Pyridinium Compounds / Q000633:toxicity; D051381:Rats
chemical_list: D000596:Amino Acids; D004140:Dinitrophenols; D007773:Lactates; D011725:Pyridines; D011726:Pyridinium Compounds; D019344:Lactic Acid; D015632:1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine; D000661:Amphetamine; D000109:Acetylcholine; D019297:2,4-Dinitrophenol; D015655:1-Methyl-4-phenylpyridinium; D004298:Dopamine