An evaluation of the role of microenvironmental factors in the limitation of myelopoiesis in murine long-term bone marrow culture.
On a day-to-day or week-to-week basis, generation of colony-stimulating factor (CSF) by the adherent layer was identical in long-term bone marrow cultures initiated from young or old mice. CSF concentration increased as myelopoiesis failed, no decline in the generation of CSF following increased stimulation was seen, and donor age did not affect the responsiveness of myeloid cells to CSF. Following spontaneous failure of myelopoiesis, adherent layers originally initiated from young or old mice were equally able to support hematopoiesis following recharge. Thus no age-related decline in adherent layer function could be detected.
Udupa
K B
KB
GRECC, John L. McClellan V.A. Hospital, Little Rock, AR 72205.
Lipschitz
D A
DA
D000367:Age Factors; D000818:Animals; D001853:Bone Marrow / Q000187:drug effects / Q000378:metabolism; D001854:Bone Marrow Cells; D002448:Cell Adhesion; D002452:Cell Count; D002478:Cells, Cultured; D003115:Colony-Stimulating Factors / Q000378:metabolism; D003470:Culture Media; D004731:Endotoxins / Q000494:pharmacology; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D013997:Time Factors
D003115:Colony-Stimulating Factors; D003470:Culture Media; D004731:Endotoxins