Determination of protein size in chromatography column eluants by on-line photon correlation spectroscopy.
The dynamic light scattering technique of photon correlation spectroscopy has been used to determine biomacromolecule hydrodynamic radius in solutions flowing at rates similar to those experienced in liquid chromatographic separation systems. Such analyses can be performed rapidly (less than 5 s). The potential of the technique as an on-line noninvasive monitor for liquid chromatography is discussed.
Carr
R J
RJ
Division of Biotechnology, Centre for Applied Microbiology and Research, Wiltshire, United Kingdom.
Rarity
J G
JG
Stansfield
A G
AG
Brown
R G
RG
Clarke
D J
DJ
Atkinson
T
T
D008027:Light; D008962:Models, Theoretical; D009113:Muramidase; D011487:Protein Conformation; D011506:Proteins / Q000032:analysis; D011827:Radiation; D012542:Scattering, Radiation; D012710:Serum Albumin, Bovine / Q000032:analysis; D013057:Spectrum Analysis / Q000379:methods
D011506:Proteins; D012710:Serum Albumin, Bovine; D009113:Muramidase