title: Separation of neutral reducing oligosaccharides derived from glycoproteins by HPLC on a hydroxylated polymeric support.
abstract: A prototype hydroxylated polymeric support, Glyco-PAK N, was employed to separate a number of neutral reducing oligosaccharides derived from various glycoproteins by the hydrazinolysis/re-N-acetylation/mild acid hydrolysis procedure. General features of the separation properties of the matrix are described. A major advantage of the packing over silica-based supports is its stability, a property which is advantageous both in repetitive analytical chromatography and in the preparative isolation and spectroscopic identification of pure compounds, free of column breakdown products.
authors.affiliation: Department of Biochemistry, Hospital for Sick Children, Toronto, Ontario, Canada.
authors.forename: B
authors.initials: B
authors.lastname: Bendiak
authors.forename: J
authors.initials: J
authors.lastname: Orr
authors.forename: I
authors.initials: I
authors.lastname: Brockhausen
authors.forename: G
authors.initials: G
authors.lastname: Vella
authors.forename: C
authors.initials: C
authors.lastname: Phoebe
mesh_terms: D000097:Acetonitriles; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006023:Glycoproteins / Q000302:isolation & purification*; D006868:Hydrolysis; D006900:Hydroxylation; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000302:isolation & purification*
chemical_list: D000097:Acetonitriles; D006023:Glycoproteins; D009844:Oligosaccharides