Macromolecular organization of natural and recombinant lung surfactant protein SP 28-36. Structural homology with the complement factor C1q.
The macromolecular structure of the pulmonary surfactant apolipoprotein SP 28-36 has been determined. For SP 28-36 isolated from dog lung lavage, a flower bouquet-like hexameric structure with six globular domains connected by short stalks to a common stem was revealed by electron microscopy, using the rotary shadowing technique. This structure is very similar to that published for the subcomponent C1q of the first component of complement C1. The lavage material was compared with the homologous human recombinant SP 28-36 by the same technique. Mostly smaller aggregates like di-, tri- and tetramers as well as very high aggregates were observed. Mild reduction of the recombinant material revealed the lollipop-shaped monomers composed of a globular domain and a tail with a discrete kink in the middle portion. The collagenous nature of the tail was demonstrated by circular dichroism spectroscopy. This implies that the mammalian expression system assembles the monomeric subunits correctly. Assembly into the hexameric structures, however, does not proceed quantitatively.
Voss
T
T
Abteilung für Molekularbiologie, Byk Gulden Pharmazeutika, Konstanz, FRG.
Eistetter
H
H
Schäfer
K P
KP
Engel
J
J
D000818:Animals; D001059:Apoproteins; D002942:Circular Dichroism; D003166:Complement Activating Enzymes; D003172:Complement C1; D015922:Complement C1q; D004285:Dogs; D006801:Humans; D046911:Macromolecular Substances; D008854:Microscopy, Electron; D011487:Protein Conformation; D037661:Pulmonary Surfactant-Associated Proteins; D011663:Pulmonary Surfactants; D011994:Recombinant Proteins; D007507:Therapeutic Irrigation
D001059:Apoproteins; D003172:Complement C1; D046911:Macromolecular Substances; D037661:Pulmonary Surfactant-Associated Proteins; D011663:Pulmonary Surfactants; D011994:Recombinant Proteins; C046616:pulmonary surfactant apoprotein; D015922:Complement C1q; D003166:Complement Activating Enzymes