Human immune response to allergens of house dust mite, Dermatophagoides pteronyssinus. V. Auto-anti-idiotypic antibody characterization and cross-reactivity.
From the serum of 10 allergic subjects we have prepared IgG antibodies recognizing idiotopes carried by specific antibodies to Dermatophagoides pteronyssinus (Dpt) allergens, and studied cross-reactivity of anti-Dpt IgG bystander and antigen-binding site-associated idiotopes by latex agglutination assays. Idiotopes of specific anti-Dpt IgE were evaluated by radioimmunoassays. Depending on the assay, a binding or inhibition of more than 50%, as compared to the reactivity of specific antibodies with the corresponding anti-idiotypic (anti-Id) IgG, was considered significant. Cross-reactivity of antigen-binding site-associated idiotopes attained a mean proportion of 6/10 for IgG and 9.6/10 for IgE. By contrast, bystander idiotopes cross-reacted only occasionally with a mean proportion of 2/10 for both IgG and IgE antibodies. Anti-Id antibodies from two subjects have been isolated by adsorption on insolubilized anti-Dpt antibodies of the corresponding patient. Using this purified material we have confirmed that (a) the majority of anti-Id antibodies carry an "internal image" of the initial antigen and compete in a dose-dependent manner with Dpt allergens for the binding to the anti-Dpt antibodies and (b) paratope-associated idiotopes of anti-Dpt antibodies are shared by unrelated individuals.
Saint-Remy
J M
JM
Experimental Medicine Unit, Université Catholique de Louvain, Brussels, Belgium.
Lebecque
S J
SJ
Lebrun
P M
PM
Jacquemin
M G
MG
D000485:Allergens / Q000276:immunology*; D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D001323:Autoantibodies / Q000276:immunology*; D001666:Binding Sites, Antibody / Q000276:immunology; D003429:Cross Reactions; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology*; D007073:Immunoglobulin E / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D008925:Mites / Q000276:immunology*
D000485:Allergens; D000888:Antibodies, Anti-Idiotypic; D001323:Autoantibodies; D007074:Immunoglobulin G; D007130:Immunoglobulin Idiotypes; D007073:Immunoglobulin E