title: Trypanosoma brucei brucei: the catabolism of glycolytic intermediates by digitonin-permeabilized bloodstream trypomastigotes and some aspects of regulation of anaerobic glycolysis.
abstract: 1. The production of pyruvate, glycerol and glycerol-3-phosphate by intact and digitonin-permeabilized Trypanosoma brucei brucei has been studied with glucose or the glycolytic intermediates as substrates. 2. Under aerobic conditions hexosephosphates gave maximal glycolysis in the presence of 40-60 micrograms digitonin/10(8) trypanosomes while the triosephosphates gave it at 20-30 micrograms digitonin/10(8) trypanosomes. 3. In the presence of salicylhydroxamic acid, and the glycolytic intermediates, permeabilized trypanosomes produced equimolar amounts of pyruvate and glycerol-3-phosphate and no glycerol. Under the same conditions, glucose catabolism produced glycerol in addition to pyruvated and glycerol-3-phosphate. 4. In the presence of salicylhydroxamic acid and ATP or ADP intact trypanosomes produced equimolar amounts of pyruvate and (glycerol plus glycerol-3-phosphate) with glucose as substrate. 5. A carrier for ATP and ADP at the glycosomal membrane is implicated. 6. It is apparent that glycerol formation is regulated by the ATP/ADP ratio and that it needs intact glycosomal membrane and the presence of glucose.
authors.affiliation: Department of Biochemistry, University of Nairobi, Kenya.
authors.forename: J K
authors.initials: JK
authors.lastname: Kiaira
authors.forename: R M
authors.initials: RM
authors.lastname: Njogu
mesh_terms: D000244:Adenosine Diphosphate / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000494:pharmacology; D000693:Anaerobiosis; D000818:Animals; D002463:Cell Membrane Permeability; D004072:Digitonin / Q000494:pharmacology*; D005947:Glucose / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D005994:Glycerophosphates / Q000378:metabolism; D006019:Glycolysis / Q000187:drug effects*; D011773:Pyruvates / Q000378:metabolism; D051381:Rats; D012457:Salicylamides / Q000494:pharmacology; D014346:Trypanosoma brucei brucei / Q000187:drug effects / Q000378:metabolism*; D014353:Trypanosomiasis, African / Q000469:parasitology*
chemical_list: D005994:Glycerophosphates; D011773:Pyruvates; D012457:Salicylamides; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; C005703:salicylhydroxamic acid; D005947:Glucose; D004072:Digitonin; D005990:Glycerol