title: Studies on the immobilization of glucuronidase (Part 2). Cleavage of hardly soluble substrates in organic solvents.
abstract: Naturally occurring glucuronides and glucosides dissolved in organic solvents can be split with the help of beta-glucuronidase (EC 3.2.1.31) immobilized on controlled pore glass. To protect the enzyme against denaturation by the organic solvents and to promote hydrolytic cleavage of substrates, two methods were used: (a) Immobilization via crosslinking with aged glutaraldehyde in presence of bovine serum albumin; and (b) Adsorption of wet enzyme to the carrier in the presence of organic solvents.
authors.affiliation: Institut für Pharmakognosie, Wien, Austria.
authors.forename: E
authors.initials: E
authors.lastname: Rapatz
authors.forename: M
authors.initials: M
authors.lastname: Ambros
authors.forename: B
authors.initials: B
authors.lastname: Kopp
authors.forename: F
authors.initials: F
authors.lastname: Pittner
mesh_terms: D003432:Cross-Linking Reagents; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000378:metabolism*; D005960:Glucosides / Q000378:metabolism; D005965:Glucuronates / Q000378:metabolism; D005966:Glucuronidase / Q000378:metabolism*; D006868:Hydrolysis; D011506:Proteins / Q000032:analysis; D012995:Solubility; D012997:Solvents
chemical_list: D003432:Cross-Linking Reagents; D004800:Enzymes, Immobilized; D005960:Glucosides; D005965:Glucuronates; D011506:Proteins; D012997:Solvents; D005966:Glucuronidase