title: Sexual immaturity and maternal age: incidence of aneuploidy and polyploidy in first-cleavage mouse embryos.
abstract: The influence of maternal age on the incidence of aneuploidy and polyploidy was studied, using C57Bl/6J X CBA/Ca hybrid mice, including immature females, as gamete donors. The age of the females ranged from 3.5-4 wk (immature or prepubertal), to 10-12 wk (young adults), to 24-28 wk (aged females). Ovulation was induced with gonadotrophins, and the differential condensation of paternal and maternal chromosomes was used to elucidate the origin of chromosome abnormalities in first-division metaphase plates. The results indicated a high incidence of aneuploid oocytes in immature and older female mice, as compared to young adult females. Eggs of immature female mice underwent polyspermic fertilization more often than those of young adults and older females, and the production of diploid oocytes was more frequent in immature females than in the other age groups.
authors.affiliation: Department of Cell Biology, Autonomous University of Barcelona.
authors.forename: V
authors.initials: V
authors.lastname: Catala
authors.forename: A M
authors.initials: AM
authors.lastname: Estop
authors.forename: J
authors.initials: J
authors.lastname: Santalo
authors.forename: J
authors.initials: J
authors.lastname: Egozcue
mesh_terms: D000367:Age Factors; D000818:Animals; D002970:Cleavage Stage, Ovum / Q000166:cytology*; D004622:Embryo, Mammalian / Q000166:cytology*; D005260:Female; D008297:Male; D008423:Maternal Age; D051379:Mice; D009865:Oocytes / Q000166:cytology; D011003:Ploidies; D011247:Pregnancy; D012741:Sexual Maturation; D013094:Spermatozoa / Q000166:cytology