Utilization of medium-chain triglycerides and octanoic acid by cultured human fibroblasts and lymphoblasts.
Cultured human fibroblasts and lymphoblasts were incubated with emulsions containing 14C-trioctanoin or 14C-tripalmitin. Both cell types were able to hydrolyse the medium-chain triglyceride but not the long-chain triglyceride to the corresponding fatty acids. At the end of a 3 days incubation period, 25-30% of the initial amount of 10 nmol/ml trioctanoin were present as triglyceride. The observed hydrolysis seems to be mediated by an esterase secreted into the culture medium, as was shown by the use of cell-conditioned medium. CO2 production from octanoic acid was below 2 nmol per mg protein and day, demonstrating that these cells have a low capacity to use this substrate for their energy metabolism.
Lemmen
C
C
Medizinische Poliklinik, Universität München, Garching.
Stuhler
K
K
Lemmen
P
P
Wolfram
G
G
D000818:Animals; D002210:Caprylates / Q000378:metabolism*; D002250:Carbon Radioisotopes; D002478:Cells, Cultured; D003571:Cytochalasin B / Q000494:pharmacology; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D009132:Muscles / Q000378:metabolism*; D010084:Oxidation-Reduction; D051381:Rats; D012867:Skin / Q000378:metabolism; D014280:Triglycerides / Q000378:metabolism*
D002210:Caprylates; D002250:Carbon Radioisotopes; D014280:Triglycerides; D003571:Cytochalasin B; C003637:tricaprylin; C005859:tripalmitin