Evidence for extracellular deamination of adenosine in the rat heart.
1. In rat heart perfused with adenosine (10(-6) M), dilazep (10(-4) M) inhibited incorporation of adenosine into nucleotides (an index of nucleoside transport and phosphorylation) to a greater extent (70%) than metabolism to inosine and uric acid (40%) and actually increased the recovery of inosine to 30% of the adenosine infused. 2. Extrapolating for complete inhibition of transport suggested that 60% of adenosine metabolism was intracellular and 40% extracellular. 3. Static incubations of atria also gave an estimate for extracellular metabolism of 40%. 4. Adenosine deaminase was localised by immunocytochemistry to the extracellular surface of endothelial cells of small coronary arteries. 5. Extracellular deamination may explain the lack of effect of nucleoside transport inhibitors on responses to adenosine in rat heart.
Meghji
P
P
Department of Cardiology, University of Wales College of Medicine, Heath Park, Cardiff, U.K.
Middleton
K
K
Hassall
C J
CJ
Phillips
M I
MI
Newby
A C
AC
D000241:Adenosine / Q000378:metabolism*; D000243:Adenosine Deaminase / Q000032:analysis; D000818:Animals; D003600:Cytosol / Q000201:enzymology; D003641:Deamination; D004109:Dilazep / Q000494:pharmacology; D007150:Immunohistochemistry; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D010477:Perfusion; D051381:Rats
D000243:Adenosine Deaminase; D004109:Dilazep; D000241:Adenosine