title: Oxygen activation and ligand binding by pure heme-octapeptide microperoxidase-8 (MP-8).
abstract: The rapid, efficient preparation of pure microperoxidase-8 (MP-8) is described. Ligand binding studies confirm that MP-8 is monomeric in alkaline solution. It is shown that the monomeric MP-8 activates oxygen in a similar manner to that already reported for alkaline hemin, establishing the octapeptide as a possible second generation model for the oxygen activation/insertion reactions of the cytochrome P-450.
authors.affiliation: MRC Biomembrane Research Unit, University of Cape Town, Medical School, Republic of South Africa.
authors.forename: P A
authors.initials: PA
authors.lastname: Adams
authors.forename: M P
authors.initials: MP
authors.lastname: Byfield
authors.forename: R C
authors.initials: RC
authors.lastname: Milton
authors.forename: J M
authors.initials: JM
authors.lastname: Pratt
mesh_terms: D000818:Animals; D006736:Horses; D008024:Ligands; D010100:Oxygen / Q000378:metabolism*; D010544:Peroxidases / Q000302:isolation & purification / Q000378:metabolism*; D013552:Swine
chemical_list: D008024:Ligands; D010544:Peroxidases; C021799:microperoxidase; D010100:Oxygen