Release of fatty acid-binding protein from isolated rat heart subjected to ischemia and reperfusion or to the calcium paradox.
The release of cardiac fatty acid-binding protein (cFABP) and of fatty acids from isolated rat hearts was measured during both reperfusion following 60 min of ischemia and the calcium paradox (readmission of Ca2+ after a period of Ca2+-free perfusion). Total cFABP release was much more pronounced after Ca2+ readmission (over 50% of tissue content) than during post-ischemic reperfusion (on average, 3% of tissue content), but in both cases, it closely paralleled the release of lactate dehydrogenase. Only minor amounts of long-chain fatty acids, if any, were released from the heart. These observations are challenging the idea that cFABP plays a fatty acid-buffering role under the pathophysiological conditions studied.
Glatz
J F
JF
Department of Physiology, University of Limburg, Maastricht, The Netherlands.
van Bilsen
M
M
Paulussen
R J
RJ
Veerkamp
J H
JH
van der Vusse
G J
GJ
Reneman
R S
RS
D000818:Animals; D002118:Calcium / Q000494:pharmacology / Q000502:physiology*; D002352:Carrier Proteins / Q000378:metabolism*; D003327:Coronary Disease / Q000378:metabolism; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D006321:Heart / Q000187:drug effects; D066298:In Vitro Techniques; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008297:Male; D008954:Models, Biological; D009206:Myocardium / Q000378:metabolism*; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D010477:Perfusion; D051381:Rats; D011917:Rats, Inbred Lew
D002352:Carrier Proteins; C490174:Fabp7 protein, rat; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D007770:L-Lactate Dehydrogenase; D002118:Calcium