Production of B cell stimulatory factor-2/interleukin-6 activity by human endothelial cells.
The effect of culture supernatants of endothelial cell (EC) lines on the immunoglobulin-M(IgM) synthesis by human B cell line, SKW6-CL4 cells, was investigated. Supernatants of human EC stimulated IgM synthesis, as high as 6-fold, but supernatants of bovine EC did not. This enhancing activity was completely blocked by addition of anti-human B cell stimulatory factor-2/interleukin-6 (BSF-2/IL-6) antibody. These data suggest that human EC might participate in the human antibody production system by producing soluble factor, BSF-2/IL-6.
Norioka
K
K
First Department of Internal Medicine, National Defense Medical College, Saitama, Japan.
Hara
M
M
Harigai
M
M
Kitani
A
A
Hirose
T
T
Suzuki
K
K
Kawakami
M
M
Tabata
H
H
Kawagoe
M
M
Nakamura
H
H
D000818:Animals; D000906:Antibodies; D002460:Cell Line; D004730:Endothelium, Vascular / Q000378:metabolism*; D006801:Humans; D007075:Immunoglobulin M / Q000096:biosynthesis; D015850:Interleukin-6; D007378:Interleukins / Q000096:biosynthesis*; D011817:Rabbits
D000906:Antibodies; D007075:Immunoglobulin M; D015850:Interleukin-6; D007378:Interleukins