Detection of arthritogenic factor in adjuvant arthritis.
An evocation of arthritis by an Ag-specific lymphokine has recently been considered with the description of arthritogenic factor (AF) in rats with collagen arthritis. Because rats with CFA-induced arthritis also exhibit T cell reactivity to native type II collagen, T cell lines specific for this protein were established from CFA-injected rats. Supernatant material from these lines contained a type II collagen-binding lymphokine with functional and biochemical attributes identical with those described for AF, i.e., it was a 65-kDa species cross-reacting immunoprotein possessing the ability to incite an erosive, proliferative synovitis when injected into the knee joint of naive recipients. Similarities were also observed with HPLC and on two-dimensional gels. Lymph node cells from rats with arthritis created by injection of the synthetic adjuvant, CP-20,961 failed to produce AF, suggesting that this material is not a ubiquitous concomitant of inflammatory arthritis in the rat. Test injections into sites contiguous with the ear cartilage plate and into fibroblast-lined s.c. pouches suggested that cartilage was a requisite for the induction of inflammation by AF. These data identify a potentially shared effector pathway in the collagen and adjuvant models. The presence of AF in two frequently used models further supports the hypothesis that Ag-specific lymphokines can create autoimmune disease.
Helfgott
S M
SM
Department of Medicine, Harvard Medical School, Beth Israel Hospital, Boston, MA 02215.
Kieval
R I
RI
Breedveld
F C
FC
Brahn
E
E
Young
C T
CT
Dynesius-Trentham
R
R
Trentham
D E
DE
D000818:Animals; D001168:Arthritis / Q000378:metabolism*; D001169:Arthritis, Experimental / Q000378:metabolism*; D002356:Cartilage / Q000503:physiopathology; D002460:Cell Line; D003094:Collagen / Q000378:metabolism; D003959:Diamines / Q000633:toxicity; D005260:Female; D005620:Freund's Adjuvant / Q000633:toxicity; D008222:Lymphokines / Q000302:isolation & purification* / Q000378:metabolism / Q000633:toxicity; D008297:Male; D051379:Mice; D008811:Mice, Inbred DBA; D051381:Rats; D011917:Rats, Inbred Lew; D011920:Rats, Inbred WF; D013585:Synovitis / Q000139:chemically induced; D013601:T-Lymphocytes / Q000032:analysis*
D003959:Diamines; D008222:Lymphokines; D003094:Collagen; D005620:Freund's Adjuvant; C006270:avridine