title: Is the activity-linked electrostatic gradient of bovine Cu, Zn superoxide dismutases conserved in homologous enzymes irrespective of the number and distribution of charges?
abstract: Electrostatic potential calculations have been performed on three different Cu, Zn superoxide dismutases (superoxide: superoxide oxidoreductase, EC 1.15. 1.1), in order to evaluate the degree of conservation of the pattern of electrostatic interactions between O2- and the active site recently pointed out in bovine Cu Zn SOD. The three Cu, Zn SODs that have been selected for this study, namely the bovine, ovine, and porcine enzymes, are highly homologous as to reasonably assume identical three-dimensional structure but display large differences in their net charge, as shown by their pI's, which span over a wide pH range: 8.0 (sheep), 6.5 (pig), 5.2 (ox). Despite such a large difference in the net protein charge and in the spatial arrangement of electrostatic charges, electrostatic potential calculations show that the electrostatic channel directing the negatively charged substrate toward the positive catalytic site is strictly preserved with the same features for the three proteins. This suggests that the electrostatic funnel for conducting small anions into the active site is a highly conservative property in the evolution of Cu, Zn SOD.
authors.affiliation: Department of Biology Tor Vergata University, Rome, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Desideri
authors.forename: M
authors.initials: M
authors.lastname: Falconi
authors.forename: V
authors.initials: V
authors.lastname: Parisi
authors.forename: S
authors.initials: S
authors.lastname: Morante
authors.forename: G
authors.initials: G
authors.lastname: Rotilio
mesh_terms: D000818:Animals; D001665:Binding Sites; D002417:Cattle; D004563:Electrochemistry; D006863:Hydrogen-Ion Concentration; D007526:Isoelectric Point; D015394:Molecular Structure; D012756:Sheep; D013482:Superoxide Dismutase / Q000032:analysis*; D013552:Swine
chemical_list: D013482:Superoxide Dismutase