[Synthesis and properties of DNA duplexes containing hydrocarbon bridges instead of a nucleoside residue].
DNA duplexes 14 bp long containing an EcoRII and MvaI restriction site in which a nucleoside is substituted by 1,3-diaminopropane or 1,3-propanediol residue have been chemically synthesized. Diaminopropane bridge was introduced by the chemical ligation, whereas the oligonucleotide containing propanediol was prepared by automatic solid phase phosphoroamidite method on "Victoria-4M" synthesizer. As CD and UV spectra show, the modification destabilises the duplex by 18-20 degrees C without essential distortion of the double helix, except for increase of the conformational mobility in the modified site.
Kuznetsova
S A
SA
Volkov
E M
EM
Gromova
E S
ES
Potapov
V K
VK
Shabarova
Z A
ZA
D001483:Base Sequence; D002942:Circular Dichroism; D004247:DNA / Q000138:chemical synthesis*; D003959:Diamines; D009690:Nucleic Acid Conformation; D009692:Nucleic Acid Heteroduplexes / Q000138:chemical synthesis*; D009705:Nucleosides; D009841:Oligonucleotides / Q000032:analysis; D011409:Propylene Glycols; D013056:Spectrophotometry, Ultraviolet
D003959:Diamines; D009692:Nucleic Acid Heteroduplexes; D009705:Nucleosides; D009841:Oligonucleotides; D011409:Propylene Glycols; C041787:1,3-propanediol; D004247:DNA; C009475:trimethylenediamine
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