Multiple molecular forms of Acanthamoeba lactic dehydrogenase.
1. Acanthamoeba lactic dehydrogenase (D-lactate specific, EC 1.1.1.28) has been studied through the use of polyacrylamide gel electrophoresis (PAGE) and isoelectric focusing (PAGIEF). 2. Data from PAGE showed the presence of only a single macromolecular form of the enzyme in trophozoites, but PAGIEF data demonstrated that at least three LDH species occur. 3. Molecular weight determinations indicated a tetrameric assembly state for the enzyme. 4. Multiple molecular forms of the enzyme (isoenzymes) are encoded either by genes at two loci or by two alleles at a single LDH locus.
Ward
A P
AP
School of Science, Humberside College of Higher Education, UK.
al-Abidin
N Z
NZ
D000048:Acanthamoeba / Q000201:enzymology* / Q000235:genetics; D000483:Alleles; D000818:Animals; D007526:Isoelectric Point; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000235:genetics / Q000302:isolation & purification*; D008970:Molecular Weight; D011487:Protein Conformation
D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase