Field application of a colorimetric method of assaying chloroquine and desethylchloroquine in urine.
In a study in western Kenya of malaria-infected adult women who had been treated with chloroquine, we compared the level of chloroquine and its principal metabolite, desethylchloroquine, in urine, measured using a newly developed modified Haskins test, with the level of chloroquine in whole blood, determined by high-performance liquid chromatography. Over a 28-day follow-up period, 277 matched urine and blood samples from 81 women were evaluated. A high correlation was observed between the level of chloroquine in whole blood (in mug/l) and that of chloroquine + desethylchloroquine in urine (in mg/l). The test was easily performed and may be useful for monitoring use of chloroquine in a community and determining pre-study or post-treatment ingestion or absorption of the drug in in vivo studies of parasite sensitivity.
Steketee
R W
RW
Mount
D L
DL
Patchen
L C
LC
Williams
S B
SB
Churchill
F C
FC
Roberts
J M
JM
Kaseje
D C
DC
Brandling-Bennett
A D
AD
D000293:Adolescent; D002738:Chloroquine / Q000031:analogs & derivatives* / Q000097:blood / Q000652:urine*; D002851:Chromatography, High Pressure Liquid; D003124:Colorimetry / Q000379:methods*; D005260:Female; D006801:Humans
D002738:Chloroquine; C007278:desethylchloroquine