The regions of alpha-neurotoxin binding on the extracellular part of the alpha-subunit of human acetylcholine receptor.
A set of 18 synthetic uniform overlapping peptides spanning the entire extracellular part (residues 1-210) of the alpha-subunit of human acetylcholine receptor were studied for their binding activity of 125I-labeled alpha-bungarotoxin and cobratoxin. A major toxin-binding region was found to reside within peptide alpha 122-138. In addition, low-binding activities were obtained with peptides alpha 34-49 and alpha 194-210. It is concluded that the region within residues alpha 122-138 constitutes a universal major toxin-binding region for acetylcholine receptor of various species.
Mulac-Jericevic
B
B
Marrs McLean Department of Biochemistry, Baylor College of Medicine, Houston, Texas 77030.
Manshouri
T
T
Yokoi
T
T
Atassi
M Z
MZ
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D002038:Bungarotoxins / Q000378:metabolism; D003039:Cobra Neurotoxin Proteins / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D009498:Neurotoxins / Q000378:metabolism*; D011950:Receptors, Cholinergic / Q000378:metabolism*; D014101:Torpedo
D002038:Bungarotoxins; D003039:Cobra Neurotoxin Proteins; D009498:Neurotoxins; D011950:Receptors, Cholinergic