Qualitative study of fecal alpha 1-proteinase inhibitor in normal subjects and patients with Crohn's disease.
We applied sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting to analyze fecal alpha 1-proteinase inhibitor (alpha 1 PI) from healthy subjects and patients with Crohn's disease. A component with Mr 38,000 was characterized in normal fecal extracts as well as in six pathological samples. In these cases, the Crohn's disease activity index (CDAI), a clinical index of severity of the disease, was 170 (SEM 47). In contrast, alpha 1 PI of Mr 51,000 was detected in fecal extracts from eight patients with active Crohn's disease (CDAI = 287, SEM 39). We conclude that fecal alpha 1 PI can be considered a marker of intestinal disease activity.
Mizon
C
C
Laboratoire de Biochimie, Faculté de Pharmacie, Lille, France.
Becuwe
C
C
Balduyck
M
M
Colombel
J F
JF
Cortot
A
A
Mizon
J
J
Degand
P
P
D000293:Adolescent; D000328:Adult; D001798:Blood Proteins / Q000032:analysis*; D003424:Crohn Disease / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005243:Feces / Q000032:analysis*; D005260:Female; D006801:Humans; D007123:Immunoelectrophoresis, Two-Dimensional; D008297:Male; D008875:Middle Aged; D008970:Molecular Weight; D000515:alpha 1-Antitrypsin
D001798:Blood Proteins; D000515:alpha 1-Antitrypsin