Insulin-like growth factors and the multiplication of Tera-2, a human teratoma-derived cell line.
A human teratoma cell line (Tera-2) was grown in serum-free medium, and the population multiplication was stimulated by the addition of somatomedins/insulin-like growth factors (IGFs). Both IGF-I and IGF-II gave maximal stimulation when added daily at 10 ng ml-1. The IGFs did not substantially change the labelling index of the cells, and the IGFs appeared to exert their effect on population multiplication by increasing cell survival. Membranes isolated from Tera-2 cells displayed both type 1 and type 2 IGF receptors.
Biddle
C
C
Cancer Research Campaign Developmental Tumour Group, Zoology Department, Oxford, UK.
Li
C H
CH
Schofield
P N
PN
Tate
V E
VE
Hopkins
B
B
Engstrom
W
W
Huskisson
N S
NS
Graham
C F
CF
D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D007335:Insulin-Like Growth Factor II / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000494:pharmacology; D013002:Somatomedins / Q000494:pharmacology*; D013268:Stimulation, Chemical; D013724:Teratoma / Q000473:pathology*; D014407:Tumor Cells, Cultured / Q000187:drug effects*
D011994:Recombinant Proteins; D013002:Somatomedins; D007334:Insulin-Like Growth Factor I; D007335:Insulin-Like Growth Factor II