Independent transcriptional regulation of a single VL30 element by epidermal growth factor and activators of protein kinase C.
A single VL30 element present in the RVL-3 cell line was transcriptionally induced by both epidermal growth factor (EGF) and the protein kinase C (pkC) activators 12-O-tetradecanoylphorbol-13-acetate (TPA) and sn-1,2-dioctanoylglycerol within 5 min of stimulation. Following TPA-induced depletion of protein kinase C activity, EGF stimulation of VL30 transcription and accumulation was unaffected while TPA effects were inhibited, implying that EGF and TPA act by separable pathways.
Rodland
K D
KD
Department of Cell Biology and Anatomy, Oregon Health Sciences University, Portland 97201.
Muldoon
L L
LL
Dinh
T H
TH
Magun
B E
BE
D000818:Animals; D002460:Cell Line; D004075:Diglycerides / Q000494:pharmacology*; D004789:Enzyme Activation / Q000187:drug effects; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D005989:Glycerides / Q000494:pharmacology*; D051379:Mice; D005810:Multigene Family / Q000187:drug effects*; D011493:Protein Kinase C / Q000378:metabolism*; D013268:Stimulation, Chemical; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D014158:Transcription, Genetic / Q000187:drug effects*
D004075:Diglycerides; D005989:Glycerides; C044295:1,2-dioctanoylglycerol; D004815:Epidermal Growth Factor; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate