Chromatographic separation of putative precursors of cholestanol.
This paper describes convenient syntheses for labeled and unlabeled cholest-5-en-3-one, cholest-4-en-3-one, epicholesterol, cholest-4-en-3 beta-ol, and cholest-4-en-3 alpha-ol. The thin-layer chromatography, high-performance liquid chromatography, and gas-liquid chromatography of these compounds and of cholestanol and epicholestanol are also described. The synthesized compounds are potential precursors in the biosynthesis of cholestanol in mammals.
Batta
A K
AK
Department of Medicine, UMDNJ-New Jersey Medical School, Newark 07103.
Salen
G
G
Shefer
S
S
D002777:Cholestanols / Q000032:analysis*; D002783:Cholestenones / Q000032:analysis / Q000138:chemical synthesis; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D009904:Optical Rotation; D013261:Sterols / Q000032:analysis / Q000138:chemical synthesis*
D002777:Cholestanols; D002783:Cholestenones; D013261:Sterols; C000475:cholest-4-en-3-one