title: Ligand activation of overexpressed epidermal growth factor receptors transforms NIH 3T3 mouse fibroblasts.
abstract: The cell surface receptor for the mitogenic peptide epidermal growth factor (EGF) is involved in control of normal cell growth and may play a role in the genesis of human neoplasia such as squamous carcinoma and glioblastoma. Soft-agar growth and focus-formation experiments with NIH 3T3 mouse fibroblasts transfected with an expression plasmid demonstrated the ligand-dependent transforming potential of the human EGF receptor without structural alterations. Activation of overexpressed normal receptor alone appears to be sufficient for transformation of NIH 3T3 cells in vitro.
authors.affiliation: Department of Developmental Biology, Genentech, South San Francisco, CA 94080.
authors.forename: H
authors.initials: H
authors.lastname: Riedel
authors.forename: S
authors.initials: S
authors.lastname: Massoglia
authors.forename: J
authors.initials: J
authors.lastname: Schlessinger
authors.forename: A
authors.initials: A
authors.lastname: Ullrich
mesh_terms: D000818:Animals; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000503:physiopathology*; D004815:Epidermal Growth Factor / Q000502:physiology; D066246:ErbB Receptors / Q000502:physiology*; D005784:Gene Amplification; D005786:Gene Expression Regulation; D008024:Ligands; D051379:Mice; D008970:Molecular Weight; D010455:Peptides / Q000502:physiology; D015291:Transforming Growth Factors
chemical_list: D008024:Ligands; D010455:Peptides; D004815:Epidermal Growth Factor; D015291:Transforming Growth Factors; D066246:ErbB Receptors