title: The inhibitory effect of oligodeoxynucleotides complementary to different fragments of tRNA structure on the enzymatic binding of Phe-tRNA to poly-U-programmed eukaryotic ribosomes.
abstract: The effect of several oligodeoxynucleotides complementary to the fragments of yellow lupin tRNA(Phe) was tested in the aminoacylation of tRNA(Phe) and in the binding of Phe-tRNA(Phe) to poly-U-programmed eukaryotic ribosomes. Oligonucleotides tested in the aminoacylation test did not give any inhibition. Monomers and dimers did not have any significant influence on the binding assay, either. A different percentage of inhibition of the binding of Phe-tRNA to ribosomes has been observed for oligonucleotides. Heptamer complementary to the anticodon loop gave 100% inhibition of the binding reaction. However, the oligonucleotides complementary to both the anticodon loop and stem and longer than the heptamer were much less effective inhibitors. A high inhibitory effect was also observed for trimers and for the decamer complementary to the D-loop and CCA-end.
authors.affiliation: Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznań.
authors.forename: J
authors.initials: J
authors.lastname: Pulikowska
authors.forename: A
authors.initials: A
authors.lastname: Włodarczak
authors.forename: A
authors.initials: A
authors.lastname: Kraszewski
authors.forename: B
authors.initials: B
authors.lastname: Imiołczyk
authors.forename: T
authors.initials: T
authors.lastname: Twardowski
mesh_terms: D001483:Base Sequence; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000494:pharmacology*; D011072:Poly U / Q000378:metabolism; D012345:RNA, Transfer, Amino Acid-Specific / Q000378:metabolism*; D012360:RNA, Transfer, Phe / Q000235:genetics / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism*; D012639:Seeds / Q000378:metabolism; D013329:Structure-Activity Relationship
chemical_list: D009838:Oligodeoxyribonucleotides; D012345:RNA, Transfer, Amino Acid-Specific; D012360:RNA, Transfer, Phe; D011072:Poly U