Estrogen-dependent release of serum proteins from MCF-7 breast carcinoma cells in vitro.
The human breast carcinoma cell line MCF-7, propagated in a chemically defined medium, released alfa-1-antitrypsin and alfa-1-antichymotrypsin to the medium under the influence of estradiol. Furthermore, an estrogen-independent release of antigenic material crossreacting with alfa-1-lipoprotein was demonstrated. In the presence of estrogen, the mean concentration of these proteins in the medium was 38 micrograms/l, for alfa-1-antitrypsin and 5 micrograms/l for alfa-1-antichymotrypsin and 34 micrograms/l for alfa-1-lipoprotein. It is suggested that alfa-1-antitrypsin and alfa-1-antichymotrypsin might be oncogene products of possible significance for MCF-7 tumor growth in vivo, while alfa-1-lipoprotein could be essential for the ability of the MCF-7 cells to proliferate in a chemically defined medium in vitro.
Krøll
J
J
Department of Clinical Chemistry, University Hospital of Copenhagen, Denmark.
Briand
P
P
D001798:Blood Proteins / Q000378:metabolism*; D001943:Breast Neoplasms / Q000378:metabolism*; D002460:Cell Line; D004967:Estrogens / Q000494:pharmacology*; D006801:Humans; D008075:Lipoproteins, HDL / Q000378:metabolism; D000514:alpha 1-Antichymotrypsin / Q000378:metabolism*; D000515:alpha 1-Antitrypsin / Q000378:metabolism*
D001798:Blood Proteins; D004967:Estrogens; D008075:Lipoproteins, HDL; D000514:alpha 1-Antichymotrypsin; D000515:alpha 1-Antitrypsin