MT-4 plaque formation can distinguish cytopathic subtypes of the human immunodeficiency virus (HIV).
Using the MT-4 plaque assay, differences in the plaque-forming ability among various isolates of the human immunodeficiency virus (HIV) were observed. Kinetic studies showed that these differences reflected the enhanced ability of individual HIV to replicate rapidly in T cells and cause cytopathic changes. The plaque-forming HIV all came from patients with disease; no healthy seropositive individuals had these types of isolates. Plaque formation may be a useful assay for identifying pathogenic strains of HIV.
Tateno
M
M
Department of Medicine, University of California, School of Medicine, San Francisco 94143.
Levy
J A
JA
D000163:Acquired Immunodeficiency Syndrome / Q000382:microbiology*; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D006678:HIV / Q000254:growth & development* / Q000502:physiology; D006801:Humans; D007700:Kinetics; D013601:T-Lymphocytes / Q000382:microbiology*; D010948:Viral Plaque Assay; D014779:Virus Replication