Epidermal growth factor (EGF) in seminal plasma and prostatic gland: a radioreceptor assay.
The binding ability of five different cells in culture to mouse epidermal growth factor (mEGF) was studied. A simple radioreceptor assay (RRA) using normal rat kidney cell (NRK-52E) as a source of EGF receptor was established. Maximal binding capacity and apparent dissociation constant of NRK-52E cell were 3.36 femtomoles of mEGF bound per 10(6) cells and 2.85 x 10(-11) M, respectively. EGF-like activity (EGF-LA) in seminal plasma and in cytosols of prostatic gland and seminal vesicle was 39.6 +/- 9.9 ng/ml, 0.67 +/- 0.10 ng/mg protein (mean +/- SE) and less than 0.2 ng/mg protein, respectively. The EGF-LA in seminal plasma was considered to be derived mainly from prostatic gland because the EGF-LA in cytosol of prostatic gland was higher than that of seminal vesicle.
Kishi
H
H
Department of Urology, Shimane Medical University, Japan.
Ishibe
T
T
Usui
T
T
Miyachi
Y
Y
D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D004815:Epidermal Growth Factor / Q000032:analysis*; D008297:Male; D051379:Mice; D011467:Prostate / Q000032:analysis*; D011869:Radioligand Assay; D051381:Rats; D012661:Semen / Q000032:analysis*
D004815:Epidermal Growth Factor