Up-regulation of interleukin 4/B-cell stimulatory factor 1 receptor expression.
The expression of interleukin 4 (IL-4) receptors on resting T and B lymphocytes was enhanced 4- to 8-fold by IL-4 stimulation of these cells. Other agents such as lipopolysaccharide and anti-IgM for B cells and concanavalin A for T cells also caused increased IL-4 receptor expression, although to a somewhat smaller degree than IL-4. Using a newly developed flow cytometric analysis based on the binding of biotinylated IL-4 and phycoerythrin-streptavidin, it was observed that receptor up-regulation in a T-cell population treated with IL-4 was a feature of the majority of the T cells. Analysis of IL-4 by cross-linkage of 125I-labeled IL-4 to IL-4 receptor with disuccinimidyl suberate indicated that the IL-4-IL-4 receptor complex was the same size in the resting and up-regulated cells, implying that the same receptor species found in resting cells was up-regulated in response to IL-4.
Ohara
J
J
Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892.
Paul
W E
WE
D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D015847:Interleukin-4; D007378:Interleukins / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D008815:Mice, Inbred Strains; D019948:Receptors, Interleukin-4; D011975:Receptors, Mitogen / Q000502:physiology*; D013388:Succinimides / Q000494:pharmacology; D013601:T-Lymphocytes / Q000378:metabolism
D007378:Interleukins; D008070:Lipopolysaccharides; D019948:Receptors, Interleukin-4; D011975:Receptors, Mitogen; D013388:Succinimides; D015847:Interleukin-4; C019358:disuccinimidyl suberate