Measurement of underivatized propranolol enantiomers in serum using a cellulose-tris(3,5-dimethylphenylcarbamate) high-performance liquid chromatographic (HPLC) chiral stationary phase.
A commercially available high-performance liquid chromatographic (HPLC) chiral stationary phase (HPLC-CSP) has been used to measure serum levels of d- and l-propranolol. The HPLC-CSP is based upon cellulose-tris(3,5-dimethylcarbamate) and is able to stereochemically resolve d- and l-propranolol without precolumn derivatization using a mobile phase composed of hexane:2-propranol:N,N-dimethyloctylamine (92:8:0.01, v/v/v). Under these conditions the observed stereochemical resolution (alpha) of the two enantiomers was alpha = 2.2. A subject's concentration-time curve of the two isomers was determined following the ingestion of 160 mg racemic propranolol.
Straka
R J
RJ
Department of Clinical Pharmacy, University of Tennessee, Memphis 38163.
Lalonde
R L
RL
Wainer
I W
IW
D002482:Cellulose; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D011433:Propranolol / Q000097:blood*; D012015:Reference Standards; D015203:Reproducibility of Results; D013237:Stereoisomerism; D014325:Tromethamine
D014325:Tromethamine; D002482:Cellulose; D011433:Propranolol