Action of praziquantel on calcium transport in Hymenolepis diminuta.
Effect of praziquantel on inward and outward Ca++ fluxes was investigated in Hymenolepis diminuta in glucose supplemented balanced electrolyte solution and under conditions of glucose/Mg++ deficiency. The 45Ca++ uptake in freshly isolated worms presented, generally, a biphasic kinetics. This comprised of an initial fast uptake phase, followed by a continued slower influx. The initial fast kinetics showed insensitivity to or slight stimulation by praziquantel depending on its concentration, and such stimulatory action was particularly prominent under Mg++ deficient condition (P less than 0.01). The subsequent slower 45Ca++ uptake was, however, markedly inhibited by the drug under both these conditions (P less than 0.01). Glucose starvation of the worms resulted in abolition of the fast 45Ca++ influx phase and uniform inhibition by the praziquantel without any indication of initial stimulatory effect (P less than 0.01). The extrusion of 45Ca++ from the label preloaded worms was stimulated by praziquantel under all the conditions investigated (P less than 0.01).
Tayal
S
S
Division of Biophysics and Parasitology, Central Drug Research Institute, Lucknow.
Gupta
S
S
Katiyar
J C
JC
Sagar
P
P
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002118:Calcium / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D006926:Hymenolepis / Q000187:drug effects* / Q000378:metabolism; D007700:Kinetics; D008274:Magnesium / Q000378:metabolism; D011223:Praziquantel / Q000494:pharmacology*
D011223:Praziquantel; D008274:Magnesium; D005947:Glucose; D002118:Calcium