The use of two-dimensional gel electrophoresis in the analysis of organ-specific maize proteins.
Two-dimensional gel electrophoresis with non-equilibrium pH gradient electrophoresis in the first dimension and sodium dodecyl sulfate-polyacrylamide gels in the second dimension has been used for the analysis of organ-specific proteins in maize. The method has been used to study the whole protein pattern of developing organs and adult leaves as well as protein patterns of in vitro translation. Examples of two-dimensional immunoblotting and in vitro translation of endosperm-specific proteins are also shown. Two-dimensional gel electrophoresis appears as an essential analytical step in the identification of organ-specific proteins and for the detection of the protein products related to organ-specific genes identified by other means.
Goday
A
A
Departmamento de Genética Molecular, Centro de Investigación y Desarrollo, CSIC, Barcelona, Spain.
Torrent
M
M
Ludevid
M D
MD
Puigdomènech
P
P
D002736:Chloroplasts / Q000032:analysis*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D006824:Hybridization, Genetic; D015151:Immunoblotting; D010940:Plant Proteins / Q000032:analysis*; D011061:Poly A / Q000302:isolation & purification; D011132:Polyribosomes / Q000032:analysis; D014176:Protein Biosynthesis; D012639:Seeds / Q000032:analysis; D003313:Zea mays / Q000032:analysis* / Q000235:genetics
D010940:Plant Proteins; D011061:Poly A