title: Liquid chromatographic determination of sulfamethazine in feeds.
abstract: A reverse-phase liquid chromatographic method for the assay of sulfamethazine (SMZ) in feeds is described. Feed samples are extracted with 50% methanol solution, centrifuged, filtered, and diluted when necessary, and chromatographed on a C-18 column. Samples are eluted with a mobile phase of 20% methanol and 80% of a solution containing acetic acid and tetramethylammonium chloride. The average recovery from spiked samples was 97.2% with a coefficient of variation of 1.2%. Linearity was very good (correlation coefficient 0.9997). Within-day and between-day coefficients of variation averaged 1.3 and 2.6%, respectively. The results for samples assayed by this method compared closely with the results from the same extracts assayed by the AOAC colorimetric method.
authors.affiliation: South Dakota State University, Chemistry Department, Brookings.
authors.forename: J E
authors.initials: JE
authors.lastname: Houglum
authors.forename: R D
authors.initials: RD
authors.lastname: Larson
authors.forename: R M
authors.initials: RM
authors.lastname: Neal
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D002853:Chromatography, Liquid; D002855:Chromatography, Thin Layer; D003124:Colorimetry; D013418:Sulfamethazine / Q000032:analysis*
chemical_list: D013418:Sulfamethazine