title: A new chromogenic substrate for assay and detection of alpha-amylase.
abstract: A new soluble chromogenic substrate for alpha-amylase was prepared by coupling partially hydrolyzed starch with a dye, Ostazin brilliant red H-3B. The substrate is precipitable from buffered solutions with ethanol and is equally suitable for assay of alpha-amylase, detection of separated alpha-amylase isoenzymes in gels, and selection of microbial producers of the enzyme.
authors.affiliation: Institute of Chemistry, Slovak Academy of Sciences, Bratislava, Czechoslovakia.
authors.forename: P
authors.initials: P
authors.lastname: Biely
authors.forename: D
authors.initials: D
authors.lastname: Mislovicová
authors.forename: O
authors.initials: O
authors.lastname: Markovic
authors.forename: V
authors.initials: V
authors.lastname: Kalác
mesh_terms: D002863:Chromogenic Compounds; D004396:Coloring Agents; D007525:Isoelectric Focusing; D013213:Starch; D014227:Triazines; D000516:alpha-Amylases / Q000032:analysis*
chemical_list: D002863:Chromogenic Compounds; D004396:Coloring Agents; D014227:Triazines; C011535:procion brilliant red H 3B; D013213:Starch; D000516:alpha-Amylases