Reliability of biotinylated DNA probes in colony hybridization: evaluation of an improved colony lysis method for detection of TEM-1 beta-lactamase.
Utilizing an improved method for colony hybridization developed by Haas & Fleming, biotin and 32P-labelled TEM-1 probes were compared for sensitivity and specificity in identifying the type of beta-lactamase made by over 100 clinical bacterial isolates. The new procedure was more reliable than a standard one, but still gave more than 20% false positive and false negative reactions.
Huovinen
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S
Massachusetts General Hospital, Boston 02114.
Huovinen
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P
Jacoby
G A
GA
D001419:Bacteria / Q000145:classification / Q000201:enzymology*; D001426:Bacterial Proteins / Q000032:analysis* / Q000235:genetics; D001710:Biotin; D004274:DNA, Recombinant; D004352:Drug Resistance, Microbial; D007525:Isoelectric Focusing; D009693:Nucleic Acid Hybridization; D011237:Predictive Value of Tests; D001618:beta-Lactamases / Q000032:analysis* / Q000235:genetics
D001426:Bacterial Proteins; D004274:DNA, Recombinant; D001710:Biotin; D001618:beta-Lactamases; C034413:beta-lactamase TEM-1