Profiling of plasma cholesterol ester and triglyceride fatty acids as their methyl esters by capillary gas chromatography, preceded by a rapid aminopropyl-silica column chromatographic separation of lipid classes.
A rapid procedure for the isolation of plasma cholesterol ester and triglyceride fractions with aminopropyl-silica columns, followed by analysis of their fatty acid compositions by capillary gas chromatography with flame ionization detection, is described. Within-series and long-term (six months) series-to-series precision were investigated. The isolation procedure caused minimal cross-over between the two lipid classes. Reference values for 57 apparently healthy Dutch adults were established and compared with data reported from other countries. Feeding of rats with four diets differing in their fatty acid compositions showed the relationship between the composition of the fatty acids in the diet and those esterified to cholesterol in plasma. The method is of potential usefulness to the establishment of the compliance of dietary interventions and basic dietary experiments.
Hoving
E B
EB
Central Laboratory for Clinical Chemistry, University Hospital, Groningen, The Netherlands.
Jansen
G
G
Volmer
M
M
Van Doormaal
J J
JJ
Muskiet
F A
FA
D000328:Adult; D000818:Animals; D002788:Cholesterol Esters / Q000097:blood*; D002849:Chromatography, Gas; D005227:Fatty Acids / Q000097:blood*; D005260:Female; D006801:Humans; D008055:Lipids / Q000097:blood; D008297:Male; D008738:Methyl Ethers / Q000097:blood*; D008875:Middle Aged; D051381:Rats; D014280:Triglycerides / Q000097:blood*
D002788:Cholesterol Esters; D005227:Fatty Acids; D008055:Lipids; D008738:Methyl Ethers; D014280:Triglycerides