title: Synthesis and rapid purification of UDP-[6-3H]galactose.
abstract: UDP[6-3H]galactose of high specificity can be obtained by oxidation of the C-6 hydroxymethyl group of UDP-galactose by galactose oxidase and subsequent reduction by sodium borotritide. One-step purification of the nucleotide sugar involves anion-exchange chromatography on a Pharmacia Mono Q column. Radiolabeled UDP-N-acetylgalactosamine can also be synthesized and purified by this procedure. Both nucleotide sugars can be used for sugar incorporation studies using the appropriate glycosyltransferase.
authors.affiliation: Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, NY 10461.
authors.forename: B
authors.initials: B
authors.lastname: Fudem-Goldin
authors.forename: P
authors.initials: P
authors.lastname: Voulalas
authors.forename: G A
authors.initials: GA
authors.lastname: Orr
mesh_terms: D002852:Chromatography, Ion Exchange / Q000379:methods; D014316:Tritium; D014531:Uridine Diphosphate Galactose / Q000138:chemical synthesis* / Q000302:isolation & purification; D014539:Uridine Diphosphate Sugars / Q000138:chemical synthesis*
chemical_list: D014539:Uridine Diphosphate Sugars; D014316:Tritium; D014531:Uridine Diphosphate Galactose