title: On-line sample processing and analysis of diol compounds in biological fluids.
abstract: We developed a coupled dual column system with an optional post-column derivatization for on-line sample processing, trace enrichment and analysis of aromatic 1,2-diol and aliphatic cis-diol biomolecules (e.g. catecholamines, ribonucleosides). The fully automated high-performance liquid chromatography analyzer tolerates the direct injection of proteinaceous fluids by use of a unique bonded-phase precolumn material which allows the simultaneous performance of covalent affinity and size-exclusion chromatography.
authors.affiliation: Laboratory for Biological Chemistry, University, Paderborn, F.R.G.
authors.forename: K S
authors.initials: KS
authors.lastname: Boos
authors.forename: B
authors.initials: B
authors.lastname: Wilmers
authors.forename: E
authors.initials: E
authors.lastname: Schlimme
authors.forename: R
authors.initials: R
authors.lastname: Sauerbrey
mesh_terms: D002395:Catecholamines / Q000032:analysis* / Q000097:blood / Q000652:urine; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D008895:Milk, Human / Q000032:analysis; D012263:Ribonucleosides / Q000032:analysis* / Q000097:blood / Q000652:urine; D013050:Spectrometry, Fluorescence
chemical_list: D002395:Catecholamines; D012263:Ribonucleosides