Zinc can increase the activity of protein kinase C and contributes to its binding to plasma membranes in T lymphocytes.
In the primary structure of protein kinase C, the presence of a putative metal-binding site has been suggested (Parker, P.J., Coussens, L., Totty, N., Rhee, L., Young, S., Chen, E., Stabel, S., Waterfield, M.D., and Ullrich, A. (1986) Science 233, 853-859). In the present report, we demonstrate that the most abundant intracellular heavy metal, zinc, can increase the activity of cytosolic protein kinase C. Zinc reversibly binds the enzyme to plasma membranes, and it may contribute to the calcium-induced binding as well. The intracellular heavy metal chelator N,N,N',N'-tetrakis(2-pyridylmethyl) ethylenediamine prevents the phorbol ester- and antigen-induced translocation of protein kinase C. This effect can be totally reversed by the concomitant addition of Zn2+, while Fe2+ and Mn2+ are only partially counteractive. Our results suggest that zinc can activate protein kinase C and contributes to its binding to plasma membranes in T lymphocytes induced by Ca2+, phorbol ester, or antigen.
Csermely
P
P
Institute of Biochemistry I, Semmelweis University School of Medicine, Budapest, Hungary.
Szamel
M
M
Resch
K
K
Somogyi
J
J
D000818:Animals; D002122:Calcium Chloride / Q000494:pharmacology; D002462:Cell Membrane / Q000201:enzymology; D002614:Chelating Agents / Q000494:pharmacology; D002712:Chlorides / Q000494:pharmacology*; D003600:Cytosol / Q000201:enzymology; D007700:Kinetics; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D011485:Protein Binding; D011493:Protein Kinase C / Q000378:metabolism*; D011817:Rabbits; D013601:T-Lymphocytes / Q000201:enzymology*; D015032:Zinc / Q000494:pharmacology*; D017967:Zinc Compounds
D002614:Chelating Agents; D002712:Chlorides; D017967:Zinc Compounds; C016837:zinc chloride; D011493:Protein Kinase C; D015032:Zinc; D002122:Calcium Chloride