Fluorescence digital image analysis of the inositol trisphosphate-mediated calcium transient in single permeabilized parietal cells.
The myo-inositol 1,4,5-trisphosphate (IP3)-induced Ca2+ mobilization in single saponin-permeabilized and fura-2-loaded parietal cells was analysed by a fluorescence digital image processor. When the cells were incubated with ATP, free cytoplasmic Ca2+ concentration [( Ca2+]i) increased in some restricted cytoplasmic regions showing discontinuous plateau and in the peripheral cytoplasm showing continuous [Ca2+]i gradient towards the plasma membranes. When treated with IP3, the high plateau enlarged to the entire cytoplasm and (a) new higher plateau(s) appeared and enlarged again in a transient manner. The IP3-induced Ca2+ transient was also observed by fluorescence microphotometry of the single cells or by fluorescence spectrophotometry and 45Ca2+ uptake experiment of the cell population.
Tsunoda
Y
Y
Department of Physiology, Kansai Medical University, Osaka, Japan.
Yodozawa
S
S
Tashiro
Y
Y
D000818:Animals; D001572:Benzofurans; D002118:Calcium / Q000378:metabolism*; D002132:Calcium Radioisotopes; D003196:Computer Graphics; D005456:Fluorescent Dyes; D016257:Fura-2; D006168:Guinea Pigs; D066298:In Vitro Techniques; D015544:Inositol 1,4,5-Trisphosphate; D007295:Inositol Phosphates / Q000494:pharmacology*; D007700:Kinetics; D008954:Models, Biological; D010295:Parietal Cells, Gastric / Q000187:drug effects / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013403:Sugar Phosphates / Q000494:pharmacology*
D001572:Benzofurans; D002132:Calcium Radioisotopes; D005456:Fluorescent Dyes; D007295:Inositol Phosphates; D013403:Sugar Phosphates; D015544:Inositol 1,4,5-Trisphosphate; D002118:Calcium; D016257:Fura-2