Localization of calcitonin and calcitonin gene-related peptide mRNAs in rat parafollicular cells by hybridocytochemistry.
By use of appropriate fragments of CT DNA or a CGRP DNA and SP6 polymerase system, we produced anti-sense RNA probes labeled with biotinylated 11-UTP. The labeling and specificity of the RNA probes were confirmed using dot-blot hybridization. By use of hybridocytochemistry, CT mRNA and CGRP mRNA were localized in all parafollicular cells in control and dihydrotachysterin-pre-treated rats. We concluded that all parafollicular cells simultaneously produce both CT mRNA and CGRP mRNA, either under control conditions or after stimulation by dihydrotachysterin-induced hypercalcemia.
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Institut of Pathology, University of Hamburg, Federal Republic of Germany.
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D000818:Animals; D001710:Biotin; D002116:Calcitonin / Q000235:genetics*; D015740:Calcitonin Gene-Related Peptide; D007150:Immunohistochemistry; D009479:Neuropeptides / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D013961:Thyroid Gland / Q000032:analysis
D009479:Neuropeptides; D012333:RNA, Messenger; D001710:Biotin; D002116:Calcitonin; D015740:Calcitonin Gene-Related Peptide