title: [Detection and characterization of a levansucrase and a sucrase in Pseudomonas syringae pv. phaseolicola].
abstract: Pseudomonas syringae pv. phaseolicola, a plant pathogenic pseudomonad, possesses two sucrose-splitting enzymes, a levansucrase and a sucrase. The levansucrase is found both extracellularly and intracellularly, and enzyme synthesis is independent of the carbon source. In addition to levansucrase, cells grown on sucrose contain a sucrase. The two sucrose-splitting enzymes differ in their optimum pH value and optimum temperature as well as in their substrate specificities.
authors.affiliation: Sektion Biologie der Friedrich-Schiller-Universität Jena, WB Mikrobielle Biochemie.
authors.forename: J
authors.initials: J
authors.lastname: Sauerstein
authors.forename: G
authors.initials: G
authors.lastname: Reuter
mesh_terms: D006602:Hexosyltransferases / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D011549:Pseudomonas / Q000201:enzymology*; D013379:Substrate Specificity; D013393:Sucrase / Q000032:analysis*; D013696:Temperature
chemical_list: D006602:Hexosyltransferases; C021513:levansucrase; D013393:Sucrase
vernacular_title: Nachweis und Charakterisierung einer konstitutiven Lävansucrase und einer epigenetisch regulierten Saccharase in Pseudomonas syringae pv. phaseolicola.