Effect of the elapsed time after the final antigen boost on the specificity of monoclonal antibodies produced by B cell hybridomas.
We have studied the effect of the number of days following the last antigen boost on the specificity of monoclonal antibodies produced by B cell hybridomas using spleen cells of mice immunized with human red cells of the A blood group. We showed, as previously observed by others, that the highest numbers of monoclonal anti-human red blood cells were obtained in fusions done 3 and 4 days after the final boost. However differential screening of the hybridoma cultures showed that the majority of the monoclonal antibodies reacting with the A blood group antigen were obtained in fusions done only 2 days after the last antigen injection. These results show that the delay between the final boost and the fusion experiment can influence not only the total number of antibody-secreting hybridomas but also the specificity of the antibodies produced.
Bazin
R
R
Canadian Red Cross Blood Transfusion Service, Terminus, Quebec, Canada.
Lemieux
R
R
D000017:ABO Blood-Group System / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000918:Antibody Specificity; D001402:B-Lymphocytes / Q000378:metabolism*; D002459:Cell Fusion; D017707:Erythrocyte Transfusion; D004912:Erythrocytes / Q000276:immunology; D005260:Female; D006801:Humans; D006825:Hybridomas / Q000378:metabolism*; D007117:Immunization, Secondary / Q000379:methods*; D051379:Mice; D008807:Mice, Inbred BALB C; D013997:Time Factors
D000017:ABO Blood-Group System; D000911:Antibodies, Monoclonal