Production of mouse monoclonal antibodies for the analysis of idiotypes in serum of patients with chronic lymphatic leukaemia.
In this report a simple procedure for the production of murine monoclonal antibodies (MoAb) against the idiotype of malignant B cells is described. Mice were immunized with lymphoid cells from patients with B-cell chronic lymphocytic leukaemia (B-CLL). After fusion of the spleen cells, hybridoma supernatants were screened for anti-idiotypic MoAb in ELISA with immunoglobulins obtained from tumour-cell lysates, xenohybridomas and patients' sera. The anti-idiotypic MoAb were used to study tumour cells and serum immunoglobulins (Ig) from four different patients with B-CLL. It was found that the serum IgM and IgD in one patient shared the same idiotype. Evidence is presented that IgG-secreting cell populations are not restricted to lambda-Ig-light chain-expressing B-CLL cells. With the help of anti-idiotype MoAb accurate measurements of total and idiotype-positive serum immunoglobulin levels during chemotherapy were possible.
de Rie
M A
MA
Central Laboratory of the Netherlands Red Cross Blood Transfusion Service, Amsterdam.
van Heemstra
D J
DJ
Huijgens
P C
PC
Zeijlemaker
W P
WP
Out
T A
TA
Melief
C J
CJ
von dem Borne
A E
AE
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D001402:B-Lymphocytes / Q000276:immunology*; D006801:Humans; D007072:Immunoglobulin D / Q000276:immunology; D007130:Immunoglobulin Idiotypes / Q000032:analysis*; D007075:Immunoglobulin M / Q000276:immunology; D007945:Leukemia, Lymphoid / Q000188:drug therapy / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C
D000911:Antibodies, Monoclonal; D007072:Immunoglobulin D; D007130:Immunoglobulin Idiotypes; D007075:Immunoglobulin M