Production of colony-stimulating factor from macrophages by muroctasin.
The effect of a muramyl dipeptide analog, N2-[(N-acetylmuramoyl)-L-alanyl-D-isoglutamyl]-N6-stearoyl-L-lysin e (MDP-Lys(L18), muroctasin), on the colony-stimulating factor (CSF) production was investigated in vitro. MDP-Lys(L18) stimulated murine peritoneal macrophages to induce CSF. However, the compound produced more CSF in the presence of both macrophages and T cell enriched fraction than in the absence of T cells. This phenomenon was found to be attributed to the fact that MDP-Lys(L18) also stimulated macrophages to induce interleukin 1 (IL-1) and the IL-1 induced CSF production by T cells. These findings suggest that macrophages may be the first target cells of MDP-Lys(L18) to produce CSF.
Yamaguchi
F
F
Research Institute, Daiichi Seiyaku Co., Ltd., Tokyo, Japan.
Akahane
K
K
Takashi
T
T
Tsukada
W
W
D000119:Acetylmuramyl-Alanyl-Isoglutamine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D003115:Colony-Stimulating Factors / Q000096:biosynthesis*; D005260:Female; D007375:Interleukin-1 / Q000096:biosynthesis; D008264:Macrophages / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D051379:Mice; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism
D000276:Adjuvants, Immunologic; D003115:Colony-Stimulating Factors; D007375:Interleukin-1; D000119:Acetylmuramyl-Alanyl-Isoglutamine; C036866:romurtide