Influence of bile acids on the biliary transport maximum of phenolsulfonphthalein in the rat.
1. The effect of changes in bile acid secretion induced by cholestyramine treatment or taurocholate infusion on the biliary transport maximum (Tm) of phenolsulfonphthalein (PSP) was studied in Wistar rats. 2. Five hours after oral administration of cholestyramine (1.5 g/kg bodyweight) the biliary output of bile acids decreased to 51% and bile flow to 76% of control values. The percentage of conjugated and unconjugated PSP excreted into bile and the Tm of the dye were not significantly modified by cholestyramine pretreatment. 3. Administration of sodium taurocholate at increasing rates (60-480 nmol/100 g bodyweight per min) enhanced bile flow and the biliary output of bile acids in a linear dose-related fashion. The Tm of PSP increased progressively until a maximum of 29% above the control values was reached at a taurocholate dose of 240 nmol/100 g bodyweight per min). The enhancement corresponded mainly to the unconjugated dye, the excretion of conjugated PSP not being significantly modified by the infusion of the bile acid. 4. The results indicate that bile acids can influence to some extent biliary excretion of PSP in the rat, although this component is of minor importance at low bile acid secretory rates.
Collado
P S
PS
Department of Physiology and Pharmacology, University of Salamanca, Spain.
Muñoz
M E
ME
Esteller
A
A
González
J
J
D000818:Animals; D001647:Bile Acids and Salts / Q000378:metabolism*; D001659:Biliary Tract / Q000187:drug effects / Q000378:metabolism*; D001692:Biological Transport / Q000187:drug effects; D002792:Cholestyramine Resin / Q000494:pharmacology; D066298:In Vitro Techniques; D008099:Liver / Q000032:analysis; D008297:Male; D010635:Phenolphthaleins / Q000493:pharmacokinetics*; D010637:Phenolsulfonphthalein / Q000493:pharmacokinetics*; D051381:Rats; D011919:Rats, Inbred Strains; D013656:Taurocholic Acid / Q000494:pharmacology
D001647:Bile Acids and Salts; D010635:Phenolphthaleins; D002792:Cholestyramine Resin; D013656:Taurocholic Acid; D010637:Phenolsulfonphthalein