Phagocytic labeling of leukocytes with 99mTc-albumin colloid for nuclear imaging.
A procedure is described for the phagocytic labeling of white blood cells (WBC) with high specific activity 99mTc--albumin colloid (TAC). The preparation contains approximately equal activities of granulocytes and monocytes. Heparinized whole blood (40 cm3) yields a preparation containing a total of 148-222 MBq (4-6 mCi) TAC-WBC including about 20% free TAC. The complete preparation time is 75 min. Imaging is completed 30 min to 4 h post administration of the TAC-WBC. Quality control methods and imaging protocols are described.
Marcus
C S
CS
Division of Nuclear Medicine, Harbor-UCLA Medical Center, Torrance 90509.
Kuperus
J H
JH
Butler
J A
JA
Henneman
P L
PL
Salk
R D
RD
Chen
B Y
BY
Vivian
M R
MR
Yamanaka
L M
LM
D000328:Adult; D002675:Child, Preschool; D004355:Drug Stability; D005260:Female; D006801:Humans; D007239:Infections / Q000000981:diagnostic imaging; D007249:Inflammation / Q000000981:diagnostic imaging; D007553:Isotope Labeling / Q000379:methods*; D007962:Leukocytes; D008297:Male; D008875:Middle Aged; D010587:Phagocytosis; D011786:Quality Control; D011877:Radionuclide Imaging; D013668:Technetium Tc 99m Aggregated Albumin
D013668:Technetium Tc 99m Aggregated Albumin