A human urine-derived interleukin 1 inhibitor. Homology with deoxyribonuclease I.
We have previously reported that the urine of febrile humans contained large quantities of an inhibitor of IL-1-induced murine thymocyte proliferation that was a glycoprotein between 30 and 40 kD in size. In the present study this factor has been purified to homogeneity using a sequence of eight purification steps (ammonium sulfate precipitation, ion exchange chromatography, molecular sieve chromatography, hydrophobic affinity chromatography, hydroxylapatite chromatography, fast protein liquid chromatography, and two HPLC steps). SDS-PAGE analysis indicates that the purified material is a 38-kD molecule. Evidence based on a partial amino acid sequence analysis as well as enzyme studies indicates that this inhibitor is a type of human DNase I.
Rosenstreich
D L
DL
Department of Medicine, Albert Einstein College of Medicine, Bronx, New York 10461.
Tu
J H
JH
Kinkade
P R
PR
Maurer-Fogy
I
I
Kahn
J
J
Barton
R W
RW
Farina
P R
PR
D000595:Amino Acid Sequence; D003850:Deoxyribonuclease I / Q000378:metabolism; D006023:Glycoproteins / Q000652:urine*; D006801:Humans; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1 / Q000037:antagonists & inhibitors*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011506:Proteins / Q000302:isolation & purification*; D012689:Sequence Homology, Nucleic Acid; D012795:Sialoglycoproteins
D006023:Glycoproteins; C507763:IL1RN protein, human; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1; D011506:Proteins; D012795:Sialoglycoproteins; D003850:Deoxyribonuclease I