Morphological diversity of DRC-1 positive cells: human follicular dendritic cells and their relatives.
Follicular dendritic cells (FDC) and their relatives of the human tonsils and lymph nodes were immunoelectron microscopically analyzed with a monoclonal antibody DRC-1 (DAKO) in a combination with an enzymatic isolation technique. DRC-1+ cells were largely grouped into three type A (large mono- to multinucleated, sea anemone-fashioned, bearing labyrinth structures, in germinal center), type B (oligodendritic or stellate, medium-sized, in the corona and the primary follicle) and type C (individually located, medium-sized. lymphoid cell-fashioned, outside follicles). Type C were found in hyperplastic lymphoid tissue as well as in some granulation tissue. The DRC-1+ mononuclear cells were curiously encountered in the peripheral blood of some autoimmune disease patients in contrast with no identification in healthy persons. Furthermore some DRC-1+ cells obtained from autoimmune patient were confirmed to proliferate in culture.
Terashma
K
K
Department of Pathology, School of Medicine, Yamagata Univ., Japan.
Ukai
K
K
Tajima
K
K
Yuda
F
F
Imai
Y
Y
D000911:Antibodies, Monoclonal / Q000276:immunology; D000943:Antigens, Differentiation / Q000276:immunology*; D001172:Arthritis, Rheumatoid / Q000473:pathology; D003713:Dendritic Cells / Q000276:immunology / Q000648:ultrastructure*; D006801:Humans; D007150:Immunohistochemistry; D008198:Lymph Nodes / Q000166:cytology; D008221:Lymphoid Tissue / Q000166:cytology*; D008854:Microscopy, Electron; D014066:Palatine Tonsil / Q000166:cytology
D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation