title: Effect of interleukin 1 beta on transducing mechanisms in 235-1 clonal pituitary cells. Part I: Modulation of adenylate cyclase activity.
abstract: In the present study we evaluated the effect of interleukin 1-beta on adenylate cyclase activity in 235-1 pituitary cell line. The dose-response curve of interleukin 1 beta effect on adenylate cyclase activity showed a significant inhibition of basal enzyme activity at 1 pM concentration, while the inhibition of forskolin stimulated adenylate cyclase activity was more pronounced and evident at both 0.01 and 1 pM concentrations. The action of the monokine on basal enzyme activity was almost completely reverted by polyclonal anti interleukin 1 beta antibody. The incubation of the cells for 48h with interleukin 1 beta showed a different pattern of response. The inhibitory effect of interleukin 1 beta on adenylate cyclase activity disappeared, while the highest concentration of interleukin 1 beta tested, caused a meaningful stimulation of adenylate cyclase activity which is not present in acute condition. These data show that interleukin 1-beta interacts with the cAMP-generating system in the 235-1 clonal pituitary cells.
authors.affiliation: Dept. of Pharmacology, II Medical School, University of Naples, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Schettini
authors.forename: T
authors.initials: T
authors.lastname: Florio
authors.forename: O
authors.initials: O
authors.lastname: Meucci
authors.forename: G
authors.initials: G
authors.lastname: Scala
authors.forename: E
authors.initials: E
authors.lastname: Landolfi
authors.forename: M
authors.initials: M
authors.lastname: Grimaldi
mesh_terms: D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D002999:Clone Cells / Q000187:drug effects; D005576:Colforsin / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D007375:Interleukin-1 / Q000494:pharmacology*; D010902:Pituitary Gland / Q000187:drug effects*; D051381:Rats; D015290:Second Messenger Systems / Q000187:drug effects*
chemical_list: D007375:Interleukin-1; D005576:Colforsin; D000262:Adenylyl Cyclases