Hematopoietic growth factors activate the tyrosine phosphorylation of distinct sets of proteins in interleukin-3-dependent murine cell lines.
By immunoblotting with antibodies for phosphotyrosine, we have demonstrated that the hematopoietic growth factors interleukin-2, interleukin-3, interleukin-4, and granulocyte-macrophage colony-stimulating factor stimulate the tyrosine phosphorylation of specific sets of proteins in murine hematopoietic progenitor cell lines. The stimulation of tyrosine phosphorylation is a receptor-dependent transient event. The effect of these hematopoietic growth factors on protein tyrosine phosphorylation was not mediated through protein kinase C.
Morla
A O
AO
Department of Biology, University of California-San Diego, La Jolla 92093.
Schreurs
J
J
Miyajima
A
A
Wang
J Y
JY
D000818:Animals; D002460:Cell Line; D003115:Colony-Stimulating Factors / Q000494:pharmacology*; D004789:Enzyme Activation / Q000187:drug effects; D006412:Hematopoietic Stem Cells / Q000187:drug effects / Q000378:metabolism; D007376:Interleukin-2 / Q000494:pharmacology*; D007377:Interleukin-3 / Q000494:pharmacology*; D015847:Interleukin-4; D007378:Interleukins / Q000494:pharmacology*; D051379:Mice; D010766:Phosphorylation; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*
D003115:Colony-Stimulating Factors; D007376:Interleukin-2; D007377:Interleukin-3; D007378:Interleukins; D015847:Interleukin-4; D011505:Protein-Tyrosine Kinases