title: Monokine-induced gene expression of a human endothelial cell-derived neutrophil chemotactic factor.
abstract: Monokines have been increasingly recognized as communication signals that interact with both immune and non-immune cells during inflammation. Specifically, interleukin-1 alpha (IL-1 alpha), interleukin-1 beta (IL-1 beta) and tumor necrosis factor-alpha (TNF-alpha) possess potent effector activities on various cell types. We present novel data demonstrating that human endothelial cells are a major source of a neutrophil chemotactic factor (NCF) synthesized upon stimulation with either IL-1 alpha, IL-1 beta, or TNF-alpha; but not with interleukin-6 (IL-6). Northern blot analysis demonstrated that 20 ng/ml of either IL-1 or TNF-alpha could induce endothelial cells to express significant levels of NCF mRNA, while IL-6 was not active in this system. These data demonstrate that monokines play an important role in mediating acute inflammation via induction of an endothelial cell-derived NCF.
authors.affiliation: Department of Internal Medicine, University of Michigan Medical School, Ann Arbor.
authors.forename: R M
authors.initials: RM
authors.lastname: Strieter
authors.forename: S L
authors.initials: SL
authors.lastname: Kunkel
authors.forename: H J
authors.initials: HJ
authors.lastname: Showell
authors.forename: R M
authors.initials: RM
authors.lastname: Marks
mesh_terms: D001345:Autoradiography; D001685:Biological Factors / Q000494:pharmacology*; D002630:Chemotactic Factors / Q000235:genetics*; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D003720:Densitometry; D004730:Endothelium, Vascular / Q000032:analysis / Q000187:drug effects*; D005786:Gene Expression Regulation / Q000187:drug effects*; D007375:Interleukin-1 / Q000494:pharmacology; D015846:Monokines; D009504:Neutrophils / Q000276:immunology; D012333:RNA, Messenger / Q000032:analysis; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
chemical_list: D001685:Biological Factors; D002630:Chemotactic Factors; D007375:Interleukin-1; D015846:Monokines; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha