Nonspecific lipid transfer protein (sterol carrier protein-2) is located in rat liver peroxisomes.
Intracellular localization of nonspecific lipid transfer protein (nsLTP) in rat hepatocytes was investigated by immunoblot analysis of the subcellular fractions and immunoelectron microscopy, using affinity-purified antibody against nsLTP. Immunoblot analysis showed that the protein exists in the peroxisomal and cytosolic fractions. Further study indicated that nsLTP exists in the soluble subfraction of the peroxisomes. Immunoelectron microscopic observation revealed that nsLTP is highly concentrated in the matrices of the peroxisomes. From these results, we concluded that nsLTP mainly exists in the matrix of the peroxisomes. The role of nsLTP is discussed.
Tsuneoka
M
M
Department of Physiology, Kansai Medical University, Osaka.
Yamamoto
A
A
Fujiki
Y
Y
Tashiro
Y
Y
D000818:Animals; D002352:Carrier Proteins / Q000032:analysis*; D003600:Cytosol / Q000032:analysis; D015151:Immunoblotting / Q000379:methods; D008099:Liver / Q000648:ultrastructure*; D008830:Microbodies / Q000032:analysis*; D008854:Microscopy, Electron / Q000379:methods; D010940:Plant Proteins; D051381:Rats
D002352:Carrier Proteins; D010940:Plant Proteins; C072087:sterol carrier proteins