A monoclonal anti-type II collagen antibody with cross-reactive anti-Ig activity specific for the F(ab')2 fragment.
An IgG2a hybridoma antibody (BC-10) was obtained by a myeloma fusion with lymphocytes from B10.RIII mice immunized against native bovine type II collagen. This anti-collagen monoclonal exhibited extensive cross-reactivity with several type II collagen species. BC-10 was found to have self-associating properties, but not the specificity of a typical IgG rheumatoid factor, inasmuch as this mAb bound to F(ab')2 fragments of itself and of normal mouse IgG. Self binding was inhibited by the association of BC-10 with type II collagen, and inhibition assays indicated that antibodies with the capacity to inhibit BC-10 binding to collagen were present in the sera from B10.RIII arthritic mice, but not from DBA/1 LacJ arthritic mice. Joint inflammation and histopathologic features consistent with arthritis were observed in mice injected with the BC-10 hybridoma.
Punjabi
C J
CJ
Department of Immunology, Ayerst Laboratories Research, Princeton, NJ 08543-8000.
Wood
D D
DD
Wooley
P H
PH
D000818:Animals; D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal / Q000032:analysis*; D000918:Antibody Specificity; D001169:Arthritis, Experimental / Q000209:etiology; D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D003094:Collagen / Q000276:immunology* / Q000378:metabolism; D003429:Cross Reactions; D006825:Hybridomas / Q000637:transplantation; D007140:Immunoglobulin Fab Fragments; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D007140:Immunoglobulin Fab Fragments; D003094:Collagen