An improved analytical method, based on HPLC with electrochemical detection, for monitoring exposure to 3-chloro-4-fluoroaniline.
An HPLC method for monitoring exposure of workers to 3-chloro-4-fluoroaniline (CFA) is described. It is based on the detection of a major urinary metabolite, 2-amino-4-chloro-5-fluorophenyl sulphate, and is superior to the previously adopted GC method. The limit of detection for the metabolite, 2-amino-4-chloro-5-fluorophenyl sulphate, is less than or equal to 0.01 mg/L (as CFA equivalent), with a coefficient of variation obtained over the range 1-15 mg/L of 4%. The HPLC method avoids the need for derivatization (cf. GC) and is consequently a much quicker and cheaper monitoring method. Urine samples from plant workers may be stored at 4 degrees C for a period of three months without loss of the metabolite.
Eadsforth
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Shell International Petroleum Maatschappij B.V., Health, Safety and Environmental Division, The Hague, The Netherlands.
Coveney
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Sjoe
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D000814:Aniline Compounds / Q000652:urine*; D000818:Animals; D002617:Chemical Industry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004563:Electrochemistry; D004781:Environmental Exposure; D004784:Environmental Monitoring / Q000379:methods*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D000814:Aniline Compounds; C059065:2-amino-4-chloro-5-fluorophenyl sulfate; C025654:3-chloro-4-fluoroaniline