title: Antigen-presenting cell-T cell interaction in the chicken is MHC class II antigen restricted.
abstract: The involvement of the MHC in the recognition of Ag by avian T lymphocytes was analyzed. PBL from chickens primed with keyhole limpet hemocyanin in vivo were induced to synthesize DNA in an in vitro response to specific Ag. Responding cells were T cells as judged by immunofluorescence staining. In vivo Ag-primed PBL were stimulated in vitro with specific Ag and further propagated in the presence of IL-2. Subsequent Ag-specific T cell proliferation required the presence of Ag-pulsed peripheral blood adherent cells (APC). T cell responses were restricted by the MHC of the APC; Ag presented by allogeneic APC did not support T cell proliferation. By using MHC-recombinant chicken lines, the gene products controlled by MHC class II loci were shown to restrict the T cell-APC interaction. This conclusion was substantiated by the inhibition of the Ag-specific T cell response by a mAb against chicken MHC class II gene products but not by a mAb against chicken MHC class I gene products.
authors.affiliation: Department of Medical Microbiology, Turku University, Finland.
authors.forename: O
authors.initials: O
authors.lastname: Vainio
authors.forename: T
authors.initials: T
authors.lastname: Veromaa
authors.forename: E
authors.initials: E
authors.lastname: Eerola
authors.forename: P
authors.initials: P
authors.lastname: Toivanen
authors.forename: M J
authors.initials: MJ
authors.lastname: Ratcliffe
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000938:Antigen-Presenting Cells / Q000276:immunology*; D002645:Chickens / Q000276:immunology*; D006649:Histocompatibility Antigens / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D007376:Interleukin-2 / Q000502:physiology; D008213:Lymphocyte Activation; D008211:Lymphocyte Cooperation; D008285:Major Histocompatibility Complex; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D006649:Histocompatibility Antigens; D000949:Histocompatibility Antigens Class II; D007376:Interleukin-2