title: Genetic, biochemical and molecular characterization of strains of Vibrio cholerae multiresistant to antibiotics.
abstract: In order to gain an insight into the distribution of resistance genes in Vibrio cholerae, we studied twenty-nine strains isolated from patients in Africa. Resistance to antibiotics in all strains except one was encoded by self-transferable plasmids belonging to incompatibility group Inc6-C. Tetracycline and chloramphenicol were poorly expressed in the original hosts but were easily detectable in the Escherichia coli transconjugants. Streptomycin resistance was due to synthesis of a 3"- or 6-aminoglycoside phosphotransferase. Based on MIC and hybridization data, high-level resistance to trimethoprim and O/129 was secondary to the presence of a dihydrofolate reductase of a new type, distantly related to type I activity. Our results confirm the presence in V. cholerae of beta-lactamases other than Tem-1.
authors.affiliation: Unité des Agents Antibactériens, CNRS UA 271, Institut Pasteur, Paris.
authors.forename: M
authors.initials: M
authors.lastname: Ouellette
authors.forename: G
authors.initials: G
authors.lastname: Gerbaud
authors.forename: P
authors.initials: P
authors.lastname: Courvalin
mesh_terms: D000617:Aminoglycosides / Q000494:pharmacology; D004269:DNA, Bacterial / Q000235:genetics; D004352:Drug Resistance, Microbial / Q000235:genetics*; D011815:R Factors; D012689:Sequence Homology, Nucleic Acid; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics; D014296:Trimethoprim Resistance / Q000235:genetics; D014734:Vibrio cholerae / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D001618:beta-Lactamases / Q000235:genetics
chemical_list: D000617:Aminoglycosides; D004269:DNA, Bacterial; D013762:Tetrahydrofolate Dehydrogenase; D001618:beta-Lactamases