Simultaneous determination of noradrenaline and 3-methoxy-4-hydroxyphenylglycol in plasma with high-performance liquid chromatography using electrochemical detection.
We herein report the simultaneous determination of the levels of noradrenaline (NA), and 3-methoxy-4-hydroxyphenylglycol (MHPG), a major metabolite of NA. The sample was subjected to a Sep Pak C18 cartridge prior to the NA and MHPG assay by high-performance liquid chromatography with an electrochemical detector. The results correlated well with the established methods. The average percentage of recovery was 91.2 and 98.7% for NA and MHPG, respectively. The intraassay coefficients of variation were 3.7 and 4.6% for NA and MHPG. The interassay coefficients of variation were 3.5 and 7.5% for NA and MHPG, respectively.
Okada
M
M
Department of Psychosomatic Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Mine
K
K
Noda
T
T
Kataoka
Y
Y
Nakagawa
T
T
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004563:Electrochemistry / Q000379:methods; D006018:Glycols / Q000097:blood*; D006801:Humans; D007202:Indicators and Reagents; D008734:Methoxyhydroxyphenylglycol / Q000097:blood*; D009638:Norepinephrine / Q000097:blood*
D006018:Glycols; D007202:Indicators and Reagents; D008734:Methoxyhydroxyphenylglycol; D009638:Norepinephrine