title: Human natural killer (NK) cells produce a late-acting B-cell differentiation activity.
abstract: The supernatant of unstimulated purified NKH-1 bearing human natural killer (NK) cells was found to enhance ongoing immunoglobulin synthesis. This NK-Cell supernatant (NKSN) enhanced IgE, IgG, and IgA synthesis from corresponding B-cell lines without increasing thymidine incorporation or cell number. Separation of NKH-1+ cells into CD3- or CD3+ cells showed that this activity was produced by the CD3- population. Recombinant human interleukin (IL)-1, IL-2, IL-4, interferon (INF)-beta 1, INF-gamma, granulocyte-macrophage colony-stimulating factor (GM-CSF), tumor necrosis factor (TNF)-alpha, or partially purified low molecular weight B-cell growth factor (BCGF) failed to provide the same enhancement of Ig synthesis. While the NKSN contained small amounts of IL-6 (0.1 U/ml) and IL-6 could increase Ig synthesis in vitro, the optimal IL-6 enhancement was far less than that observed with NKSN. NKSN also enhanced ongoing Ig synthesis from in vivo activated B cells obtained from peripheral blood or bone marrow but failed to induce Ig synthesis from resting or in vitro activated B cells. These results demonstrate that human NK (CD3-, NKH-1+) cells can produce B-cell differentiation activity capable of regulating Ig production in vivo, which appears to be distinct from the activity of previously described cytokines.
authors.affiliation: Department of Medicine, UCLA School of Medicine 90024.
authors.forename: H
authors.initials: H
authors.lastname: Kimata
authors.forename: E H
authors.initials: EH
authors.lastname: Sherr
authors.forename: A
authors.initials: A
authors.lastname: Saxon
mesh_terms: D000917:Antibody Formation; D001402:B-Lymphocytes / Q000378:metabolism*; D002454:Cell Differentiation; D002460:Cell Line; D006801:Humans; D007070:Immunoglobulin A / Q000096:biosynthesis; D007073:Immunoglobulin E / Q000096:biosynthesis; D007074:Immunoglobulin G / Q000096:biosynthesis; D007136:Immunoglobulins / Q000096:biosynthesis*; D007694:Killer Cells, Natural / Q000378:metabolism*; D013997:Time Factors
chemical_list: D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007136:Immunoglobulins; D007073:Immunoglobulin E