Direct evidence for granuloma-inducing activity of interleukin-1. Induction of experimental pulmonary granuloma formation in mice by interleukin-1-coupled beads.
Pulmonary granulomas were induced in BALB/c mice by the intratracheal injection of Sephadex G-50 and latex beads. Very large granulomas developed around Sephadex G-50 beads. Minimal inflammation was produced in mice given latex beads. Aqueous extracts prepared from pulmonary granuloma lesions induced in mice by Sephadex G-50 beads contained high levels of interleukin-1 (IL-1) activity but not interleukin-2 (IL-2) activity. IL-1 activity in the extracts correlated with granuloma size. In a subsequent step, large granulomas were induced by the intratracheal injection of Sepharose 4B beads coupled to fractions of the extracts containing IL-1 activity (ie, granuloma-derived IL-1) prepared from Sephadex G-50-induced granulomatous lungs. In addition, large granulomas were induced by the intratracheal injection of recombinant IL-1-coated Sepharose 4B beads. In contrast, very small granulomas were seen when IL-2-coated or plain Sepharose 4B beads were injected into mice. These results indicate that IL-1 participates in the induction and/or expression of granulomas.
Kasahara
K
K
First Department of Internal Medicine, School of Medicine, Showa University, Tokyo, Japan.
Kobayashi
K
K
Shikama
Y
Y
Yoneya
I
I
Soezima
K
K
Ide
H
H
Takahashi
T
T
D000818:Animals; D005260:Female; D006099:Granuloma / Q000139:chemically induced*; D007375:Interleukin-1 / Q000032:analysis; D008168:Lung / Q000032:analysis; D008171:Lung Diseases / Q000139:chemically induced*; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D011994:Recombinant Proteins; D014132:Trachea
D007375:Interleukin-1; D011994:Recombinant Proteins