Effects of omeprazole on rat gastric mucus glycoproteins with acetylsalicylic acid-induced gastric damage.
Investigations were carried out to determine the effects of omeprazole on mucus glycoprotein in rat gastric mucosa. Quantitative and qualitative changes in mucus glycoprotein, isolated from omeprazole-treated rats with or without acetylsalicylic acid-induced gastric damage, and the degree of gastric mucosal injury were studied. Omeprazole, in a dose-dependent manner, prevented macroscopical mucosal damage induced by acidified acetylsalicylic acid. A considerable decrease in corpus mucus glycoprotein (54%) and antral mucus glycoprotein (72%) was observed in acetylsalicylic acid-treated rats and acetylsalicylic acid reduced the amounts of fucose and galactose of corpus mucus glycoprotein. Pretreatment with omeprazole partially restored a quantitative reduction and a qualitative change of carbohydrate portion in corpus mucus glycoprotein induced by acetylsalicylic acid. A single administration of a high dose of omeprazole (200 mumol/kg) also induced the reduction of corpus mucus glycoprotein content (82%), although no changes in the carbohydrate component were observed. The decrease in corpus mucus glycoproteins induced by omeprazole might be due to a cause different from that of acetylsalicylic acid-induced mucus glycoprotein reduction.
Ohara
S
S
Department of Biochemistry, Kitasato University School of Medicine, Sagamihara, Japan.
Murayama
N
N
Kuwata
H
H
Ishihara
K
K
Hotta
K
K
D000818:Animals; D001241:Aspirin; D050260:Carbohydrate Metabolism; D005753:Gastric Mucosa / Q000187:drug effects / Q000378:metabolism*; D006023:Glycoproteins / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008297:Male; D009853:Omeprazole / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D013276:Stomach Ulcer / Q000139:chemically induced / Q000378:metabolism*
D006023:Glycoproteins; D009853:Omeprazole; D001241:Aspirin