Enzyme-linked immunosorbent assay using excretory/secretory and somatic antigens as a diagnostic test for human hookworm infection.
Excretory/secretory (E/S) and somatic antigens prepared from third stage infective larvae of Ancylostoma duodenale were evaluated for the diagnosis of human hookworm infection by enzyme-linked immunosorbent assay (ELISA). Taking the mean absorbance plus 2 standard deviations (SD) of normal serum as the cut-off value, the positivity rates in 30 hookworm cases were 97% and 93% respectively. False positivity with E/S antigen was 8-20% in 61 control subjects; with somatic antigen it was slightly lower. In contrast, when the mean absorbance value plus 2 SD of control groups with other parasitic infections was taken as cut-off point, the positivity was 93% with E/S antigen and 43% with somatic antigen, indicating the superiority of E/S antigen. Percentage positivity declined after treatment of hookworm cases. No relationship was found between ELISA reactivity and severity of infection.
Shetty
P
P
Department of Hepatology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
Dilawari
J B
JB
Virk
K J
KJ
Sehgal
R
R
Ganguly
N K
NK
Mahajan
R C
RC
D000293:Adolescent; D000328:Adult; D000368:Aged; D000722:Ancylostoma / Q000276:immunology; D000724:Ancylostomiasis / Q000175:diagnosis*; D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged
D000947:Antigens, Helminth