Autonomous healing of chicken flexor tendons in vitro.
A monoclonal antibody to procollagen type I, which specifically stains cells actively secreting collagen, was used to study the healing of chicken flexor tendons in vitro. Healing was assessed with histology, immunohistologic staining, and autoradiography. Studies showed that epitenon cells proliferate 2 days after injury and are producing collagen 4 days after injury. Endotenocytes remain dormant during this time period. Tendons in vitro demonstrate active collagen synthesis and thus the capacity for autonomous repair.
Garner
W L
WL
Department of Plastic Surgery, Washington University School of Medicine, St. Louis, MO 63110.
McDonald
J A
JA
Kuhn
C
C
Weeks
P M
PM
D000818:Animals; D000911:Antibodies, Monoclonal; D002478:Cells, Cultured; D002645:Chickens; D003094:Collagen / Q000096:biosynthesis; D005260:Female; D011347:Procollagen / Q000378:metabolism / Q000502:physiology; D013710:Tendons / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D014945:Wound Healing
D000911:Antibodies, Monoclonal; D011347:Procollagen; D003094:Collagen