High-performance liquid chromatographic evaluation of salicyloyl pyridixol and systemic metabolites in biological samples.
An high-performance liquid chromatographic analytical method, which allows quantitative evaluation of both salicyloyl pyridixol and its metabolite salicylic acid, is reported. This method has demonstrated to possess the required specifications in terms of linearity, sensitivity, extraction recovery, reproducibility and specificity for pharmacokinetic investigations in both human subjects and experimental animals. The results obtained from an investigation on the rat are briefly discussed.
Marzo
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A
Real srl, Laboratory of Drug Metabolism and Pharmacokinetics, Como, Italy.
Reiner
A
A
Monti
N
N
Ripamonti
M
M
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000097:blood*; D001565:Benzoates / Q000097:blood; D002851:Chromatography, High Pressure Liquid; D011736:Pyridoxine / Q000031:analogs & derivatives* / Q000097:blood / Q000493:pharmacokinetics / Q000652:urine; D051381:Rats; D012459:Salicylates / Q000097:blood / Q000493:pharmacokinetics* / Q000652:urine
D000894:Anti-Inflammatory Agents, Non-Steroidal; D001565:Benzoates; D012459:Salicylates; C009503:3-toluic acid; C059403:salicyloylpyridoxol; D011736:Pyridoxine