Time-resolved fluorometry for the identification of viral DNA in clinical specimens.
Time-resolved fluorometry was used for the detection of DNA probes labeled directly with europium (Eu3+) chelate. The sensitivity of this nonisotopic hybridization assay was 100 pg of homologous DNA. Equivalent results with reference tests were obtained in the detection of adenoviruses in clinical specimens.
Dahlén
P
P
Department of Virology, University of Turku, Finland.
Hurskainen
P
P
Lövgren
T
T
Hyypiä
T
T
D000260:Adenoviruses, Human / Q000302:isolation & purification*; D015342:DNA Probes; D004279:DNA, Viral / Q000032:analysis*; D005063:Europium; D005243:Feces / Q000382:microbiology; D006801:Humans; D009297:Nasal Mucosa / Q000382:microbiology; D010614:Pharynx / Q000382:microbiology; D013050:Spectrometry, Fluorescence / Q000379:methods
D015342:DNA Probes; D004279:DNA, Viral; D005063:Europium