title: [Affinity modification of DNA-dependent RNA-polymerase of phage T7 with 5'-p-fluorosulfonylbenzoyladenosine].
abstract: The affinity modification of the DNA-dependent RNA-polymerase of bacteriophage T7 was carried out by using the specific irreversible inhibitor, 5'-p-fluorosulfonylbenzoyladenosine. The inhibitor was found to bind to the enzyme's active site; the kinetic constants of the modification were calculated. The stoichiometry of the covalent E.I-complex formed was determined by using the 14C-labeled inhibitor.
authors.forename: V L
authors.initials: VL
authors.lastname: Tunitskaia
authors.forename: A A
authors.initials: AA
authors.lastname: Mishin
authors.forename: V V
authors.initials: VV
authors.lastname: Tiurkin
authors.forename: D L
authors.initials: DL
authors.lastname: Liakhov
authors.forename: V O
authors.initials: VO
authors.lastname: Rechinskiĭ
mesh_terms: D000241:Adenosine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000345:Affinity Labels; D001665:Binding Sites; D012321:DNA-Directed RNA Polymerases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D006863:Hydrogen-Ion Concentration; D010957:Plasmids; D013604:T-Phages / Q000201:enzymology*
chemical_list: D000345:Affinity Labels; C010959:5'-(4-fluorosulfonylbenzoyl)adenosine; D012321:DNA-Directed RNA Polymerases; D000241:Adenosine
vernacular_title: Affinnaia modifikatsiia DNK-zavisimoĭ RNK-polimerazy faga T7 5'-p-ftorsul'fonilbenzoiladenozinnom.