title: Fatty acids binding to albumin increases its uptake and transcytosis by the lung capillary endothelium.
abstract: To determine whether uptake and transcytosis of albumin (A) in continuous capillary endothelia are modified when this protein carries fatty acids, the transport of albumin-oleic acid and albumin-palmitic acid complexes was compared with that of defatted albumin. The probes, either radioiodinated or tagged with 5-nm gold particles (Au), or both, were perfused in situ or injected in vivo; after 3 or 30 min lung fragments were radioassayed or examined by electron microscopy. Both in situ and in vivo, the uptake of fatty acid-carrying albumin (A-FA) was consistently 2 to 3 times higher than that of defatted A. Electron microscopy revealed that A-FA complexes tagged with gold were taken up and transported across the endothelium by plasmalemmal vesicles. Morphometric analysis showed that as compared with A-Au, at 3 min the density of (A-FA)Au bound to plasmalemmal vesicles was 2 to 3 times higher, and the extent of transcytosis was increased. Uptake of the iodinated albumin was more effectively competed by A-FA complexes than by defatted A, suggesting a higher affinity of the former for the albumin binding sites of the endothelium. The results indicate that when carrying fatty acids, albumin is taken up specifically and with high affinity by the capillary endothelium, a process that may play a role in the transport of fatty acids from the plasma to the cells where they are metabolized.
authors.affiliation: Institute of Cellular Biology and Pathology, Bucharest, Romania.
authors.forename: Z
authors.initials: Z
authors.lastname: Galis
authors.forename: L
authors.initials: L
authors.lastname: Ghitescu
authors.forename: M
authors.initials: M
authors.lastname: Simionescu
mesh_terms: D000418:Albumins / Q000378:metabolism* / Q000493:pharmacokinetics; D000818:Animals; D002196:Capillaries / Q000166:cytology* / Q000502:physiology; D004730:Endothelium, Vascular / Q000378:metabolism* / Q000502:physiology / Q000648:ultrastructure; D005227:Fatty Acids / Q000378:metabolism*; D008168:Lung / Q000098:blood supply* / Q000166:cytology / Q000378:metabolism; D008297:Male; D051379:Mice; D008854:Microscopy, Electron; D010168:Palmitates / Q000493:pharmacokinetics; D011849:Radioactive Tracers / Q000493:pharmacokinetics
chemical_list: D000418:Albumins; D005227:Fatty Acids; D010168:Palmitates; D011849:Radioactive Tracers