Separation of Friend erythroleukaemic cell histones and high-mobility-group proteins by reversed-phase high-performance liquid chromatography.
A procedure for the rapid separation of histones and high-mobility-group (HMG) proteins from Friend erythroleukaemic cells (line F4N) by reversed-phase high-performance liquid chromatography is reported. By using a Nucleosil 300-5 C4 column and a multistep water-acetonitrile gradient containing 0.1% trifluoroacetic acid, the HMG-1 and HMG-2 proteins, several H1 subfractions including H1(0), H4, H2B, two H2A variants and two H3 subfractions were separated. Under changed conditions, by applying a varied acetonitrile gradient system, even two H2B variants were fractionated. The methods described seem to be a real alternative to the time-consuming polyacrylamide gel electrophoresis.
Lindner
H
H
Institute of Medical Chemistry and Biochemistry, University of Innsbruck, Austria.
Helliger
W
W
Puschendorf
B
B
D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D005622:Friend murine leukemia virus; D006609:High Mobility Group Proteins / Q000302:isolation & purification*; D006657:Histones / Q000302:isolation & purification*; D004915:Leukemia, Erythroblastic, Acute / Q000378:metabolism*
D006609:High Mobility Group Proteins; D006657:Histones