Polymerisation and crosslinking of fibrin monomers in diabetes mellitus.
Polymerisation and crosslinking of fibrin monomers was studied in 35 healthy volunteers and in 42 poorly controlled diabetic patients. Polymerisation did not show any difference between control subjects (n = 10) and diabetic patients (n = 11) (p greater than 0.1), although fibrinogen was 35% more glycated in the diabetic patients (p less than 0.001). Alpha chain crosslinking in the diabetic patients, however, was impaired as is shown from an increase in intermediate alpha polymers with a concomitant decrease in alpha monomer disappearance. A significant positive correlation was found between the degree of glycation of fibrinogen and the defective alpha chain polymerisation (r = 0.86, p less than 0.005). These results were consistent with the results of thrombin and reptilase experiments. The reaction rate with reptilase did not show any difference between the two groups (p greater than 0.1), whereas the reaction rate with thrombin was significantly slower in the diabetic group compared to the control subjects (p less than 0.001). Purified fibrin clots obtained from the diabetic patients were more susceptible to plasmin than clots obtained from control subjects. It is concluded that in poorly controlled diabetic patients polymerisation of fibrin monomers is normal, but crosslinking of the alpha chains is impaired, leading to a higher susceptibility of the clots to plasmin degradation.
Lütjens
A
A
Department of Clinical Chemistry, Andreas Ziekenhuis, Amsterdam, The Netherlands.
Jonkhoff-Slok
T W
TW
Sandkuijl
C
C
vd Veen
E A
EA
vd Meer
J
J
D000328:Adult; D001786:Blood Glucose / Q000032:analysis; D003922:Diabetes Mellitus, Type 1 / Q000097:blood*; D005337:Fibrin / Q000378:metabolism*; D005340:Fibrinogen / Q000032:analysis; D006442:Glycated Hemoglobin / Q000032:analysis; D006801:Humans; D007700:Kinetics; D046911:Macromolecular Substances; D008875:Middle Aged; D012016:Reference Values
D001786:Blood Glucose; D005337:Fibrin; D005340:Fibrinogen; D006442:Glycated Hemoglobin; D046911:Macromolecular Substances