Enhancement of antibody production to hepatitis B surface antigen by interleukin 2.
Studies were undertaken to determine whether induction of antibody to hepatitis B surface antigen (anti-HBs) production by cultured lymphocytes which had been conducted with pokeweed mitogen (PWM) could be replaced by interleukin 2 (IL 2). In cultures stimulated with purified HBsAg and IL 2, the comparable levels of anti-HBs production to those obtained in cultures stimulated with PWM alone occurred when peripheral blood mononuclear cells (PBMC) from patients positive for serum anti-HBs and recipients of HBsAg vaccine were used as effector cells. Detectable amounts of anti-HBs were produced only when IL 2 was added to the second-set cultures again. IL 2 or HBsAg alone, however, did not induce anti-HBs production. Anti-HBs production was not observed by the additions of these additives when PBMC from chronic HBsAg carriers and control individuals were used. These findings indicate that IL 2 could modulate the immune response to HBsAg.
Kakumu
S
S
Third Department of Medicine, Nagoya University School of Medicine, Japan.
Fuji
A
A
Tahara
H
H
Yoshioka
K
K
Sakamoto
N
N
D002353:Carrier State / Q000276:immunology; D006509:Hepatitis B / Q000276:immunology / Q000517:prevention & control; D006510:Hepatitis B Antibodies / Q000096:biosynthesis*; D006514:Hepatitis B Surface Antigens / Q000276:immunology*; D006801:Humans; D066298:In Vitro Techniques; D007376:Interleukin-2 / Q000008:administration & dosage / Q000494:pharmacology*; D008214:Lymphocytes / Q000276:immunology; D011043:Pokeweed Mitogens / Q000494:pharmacology; D014761:Viral Hepatitis Vaccines / Q000008:administration & dosage
D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens; D007376:Interleukin-2; D011043:Pokeweed Mitogens; D014761:Viral Hepatitis Vaccines