Gas chromatographic method for the determination of N-acetyl-S-(N-methylcarbamoyl)cysteine, a metabolite of N,N-dimethylformamide and N-methylformamide, in human urine.
A simple method has been developed for the determination of N-acetyl-S-(N-methylcarbamoyl)cysteine in human urine. Treatment of a urine sample (1 ml) with ethanol (2 ml) and potassium carbonate (1.5 g) produces ethyl N-methylcarbamate, which is extracted into ethanol and measured by packed column gas chromatography with nitrogen-sensitive detection. The limit of quantitation in human urine is 1 microgram/ml and the between-sample coefficient of variation is 5-11%. Simultaneously, N,N-dimethylformamide, N-methylformamide and formamide can also be determined.
Mráz
J
J
Institute of Hygiene and Epidemiology, Prague, Czechoslovakia.
D000111:Acetylcysteine / Q000031:analogs & derivatives* / Q000652:urine; D002849:Chromatography, Gas; D004126:Dimethylformamide / Q000652:urine*; D005559:Formamides / Q000652:urine*; D006801:Humans
D005559:Formamides; C052469:N-acetyl-S-(N-methylcarbamoyl)cysteine; D004126:Dimethylformamide; D000111:Acetylcysteine; C002950:methylformamide