The function of human intercellular adhesion molecule-1 (ICAM-1) in the generation of an immune response.
Monoclonal antibody RR 1/1 directed against the putative LFA-1 ligand molecule intracellular adhesion molecule-1 (ICAM-1) was found to inhibit the T cell proliferative response to the antigen PPD. Interestingly, the percentage of unstimulated monocytes which expressed ICAM-1 on their surface appeared to vary greatly from person to person although the majority of monocytes did express high levels of ICAM-1 within their cytoplasm and surface expression could be rapidly induced on most cells by adherence to fibronectin. Resting T cells showed no evidence of surface or cytoplasmic ICAM-1 although expression was induced both within the cell and on the membrane as a result of activation with phytohemagglutinin or a combination of OKT3 and phorbol 12,13-dibutyrate. The significance of these findings with respect to the function of monocyte and T cell in the generation of an immune response is discussed.
Dougherty
G J
GJ
Macrophage Laboratory, Imperial Cancer Research Fund, London, GB.
Murdoch
S
S
Hogg
N
N
D000328:Adult; D000911:Antibodies, Monoclonal / Q000502:physiology; D000954:Antigens, Surface / Q000276:immunology / Q000502:physiology*; D001666:Binding Sites, Antibody; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules; D002450:Cell Communication; D002460:Cell Line; D006801:Humans; D008213:Lymphocyte Activation; D009000:Monocytes / Q000032:analysis / Q000276:immunology / Q000502:physiology; D013601:T-Lymphocytes / Q000032:analysis / Q000276:immunology* / Q000502:physiology
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D015815:Cell Adhesion Molecules