T-cell antigen receptor studies in mice expressing the lpr genetic defect.
Mice having the lpr genetic defect bear an expanding lymphoid T-cell population with unusual cell surface characteristics. Using SDS-PAGE analysis, the T cells from lpr mice were shown to have two forms of the T-cell antigen receptor. A conventional (nonreduced) 90-kDa receptor and a lighter 70- to 85-kDa form were both detected. The 70- to 85-kDa antigen receptor was also shown to be present on lpr thymocytes. Only the normal 90-kDa receptor was found in MRL-++ mice. Treatment of the receptor with N-glycanase shows that the 70- to 85-kD form may be a product of abnormal glycosylation. The low-molecular-weight antigen receptor on lpr T cells is unusual and might contribute in some way to the disordered immunoregulation and autoimmunity that occur spontaneously in these mice.
Dang
H
H
Clinical Immunology Section, Audie L. Murphy Memorial Veterans Hospital, San Antonio, Texas.
Talal
N
N
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D011232:Chemical Precipitation; D008213:Lymphocyte Activation; D008562:Membrane Glycoproteins / Q000032:analysis; D051379:Mice; D008817:Mice, Mutant Strains / Q000276:immunology*; D008970:Molecular Weight; D011948:Receptors, Antigen, T-Cell / Q000032:analysis*; D013601:T-Lymphocytes / Q000032:analysis*
D000911:Antibodies, Monoclonal; D008562:Membrane Glycoproteins; D011948:Receptors, Antigen, T-Cell