Solid-phase synthesis of a nonadecapeptide coded for by the v-myb oncogene.
A nonadecapeptide comprising a predicted B-cell determinant from the v-myb oncoprotein was synthesized by Merrifield's solid-phase method. Hydrogen chloride in dichloromethane was used for protective t-butyloxycarbonyl group removal; the deprotection was monitored using a new qualitative deprotection test. The nonadecapeptide coupled to a carrier elicited a high titre of protein-reactive antipeptide antibodies.
Krchnák
V
V
Léciva-Pharmaceuticals, Czechoslovak Academy of Sciences, Prague.
Malý
A
A
Flegel
M
M
D000595:Amino Acid Sequence; D009189:Avian Myeloblastosis Virus / Q000235:genetics; D001402:B-Lymphocytes / Q000378:metabolism; D007202:Indicators and Reagents; D009842:Oligopeptides / Q000138:chemical synthesis* / Q000235:genetics; D009856:Oncogene Proteins, Viral / Q000138:chemical synthesis; D009857:Oncogenes
D007202:Indicators and Reagents; D009842:Oligopeptides; D009856:Oncogene Proteins, Viral