Effects of gossypol acetic acid on the absorptive and digestive functions of rat intestine.
Oral administration of Gossypol acetic acid (10 mg/kg body wt./day, daily for 15 days), an experimental antifertility agent to male rats, caused significant reduction in the uptake of glucose, alanine, leucine and calcium in the small intestinal segments. Gossypol also caused significant decrease in the intestinal brush border membrane--associated enzymes, sucrase, lactase, maltase and alkaline phosphatase. Kinetic analysis indicated that Gossypol decreased the apparent velocity of the disaccharidases while the Km was not altered. It also caused a shift in the transition temperature in these enzymes and predictably changed the energy of activation both below and above the transition temperature, although the Arrhenius expressions of the temperature dependence still showed proximity and were parallel to the control group.
Chadha
S
S
Department of Zoology, Panjab University, Chandigarh, India.
Sanyal
S N
SN
Kanwar
U
U
D000284:Administration, Oral; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D004063:Digestion / Q000187:drug effects*; D006072:Gossypol / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000633:toxicity; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000187:drug effects*; D007700:Kinetics; D008297:Male; D008871:Microvilli / Q000201:enzymology; D051381:Rats; D011919:Rats, Inbred Strains; D013089:Spermatocidal Agents / Q000008:administration & dosage / Q000633:toxicity*; D013816:Thermodynamics
D013089:Spermatocidal Agents; D006072:Gossypol; C028178:gossypol acetic acid