Group fractionation of free and conjugated steroids by means of disposable silica-based anion-exchange columns.
Adsorption and desorption modes have been elaborated which allow the separation of complex steroid mixtures of biological fluids into an unconjugated, a glucuronic acid conjugated and a sulphuric acid and mixed conjugated fraction. Capillary gas chromatographic analysis of the fractions obtained provides "total steroid profiles" of urine, blood, ovarian follicle fluid and amniotic fluid.
Vanluchene
E
E
University Hospital, Department of Gynaecology, State University of Ghent, Belgium.
Vandekerckhove
D
D
D002849:Chromatography, Gas; D002852:Chromatography, Ion Exchange; D002855:Chromatography, Thin Layer; D006801:Humans; D013256:Steroids / Q000032:analysis* / Q000097:blood / Q000652:urine
D013256:Steroids