Detection of plasmid-mediated beta-lactamases with DNA probes.
beta-Lactamase identification by colony hybridization with 32P-labeled DNA probes for TEM-1, SHV-1, OXA-1, OXA-2, PSE-1, PSE-2, and PSE-4 was compared with isoelectric focusing in 122 clinical isolates making a variety of enzyme types. All strains producing a probe-type enzyme gave a positive hybridization reaction. Cross-hybridization was observed between TEM-1 and TEM-2 or TLE-1, between SHV-1 and SHV-2, between OXA-1 and OXA-4, between OXA-2 and OXA-3 (weak), between PSE-2 and OXA-6 or OXA-5 (weak), and among PSE-1, PSE-4, and CARB-3. With allowance for such cross-hybridization, only six strains gave false-positive reactions, and the procedure was 99% specific.
Huovinen
S
S
Massachusetts General Hospital, Boston 02114.
Huovinén
P
P
Jacoby
G A
GA
D001419:Bacteria / Q000201:enzymology* / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics*; D005189:False Positive Reactions; D005798:Genes, Bacterial; D007525:Isoelectric Focusing; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D011237:Predictive Value of Tests; D001618:beta-Lactamases / Q000032:analysis* / Q000235:genetics
D004269:DNA, Bacterial; D001618:beta-Lactamases