Kinetics of benzo(a)pyrene hydroxylation at varying concentrations of NADPH and NADH in liver microsomes of nontreated and beta-naphthoflavone-treated C57BL/2 and DBA/2 mice.
The biphasic kinetics of hydroxylation of benzo(a)pyrene (B(a)P) dependent on the concentration of either NADPH or NADH has been observed in DBA/2 (AhdAhd) but not in C57BL/6 (AhbAhb) beta-naphthoflavone-treated mice. On the other hand, in nontreated mice, this biphasic kinetics has been observed in both strains of mice. This shows that characteristics of biphasic kinetics differentiate Ahb from Ahd mice only after treatment with methylcholanthrene-type of inducers. The discussed biphasic kinetics was more regular and distinct in the NADH-dependent reaction as compared with NADPH-dependent hydroxylation of B(a)P. It is suggested that the NADH-specific pathway of electron transport to cytochrome P-450 is necessary for the occurrence of this effect both in NADH- and NADPH supported reaction.
Brauze
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Institute of Human Genetics, Polish Academy of Sciences, Poznań, Poland.
Pawlak
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AL
D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases / Q000378:metabolism*; D001571:Benzoflavones / Q000494:pharmacology; D001579:Benzopyrene Hydroxylase / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008862:Microsomes, Liver / Q000201:enzymology*; D009243:NAD / Q000494:pharmacology; D009249:NADP / Q000494:pharmacology; D019324:beta-Naphthoflavone
D001571:Benzoflavones; D009243:NAD; D009249:NADP; D019324:beta-Naphthoflavone; D001579:Benzopyrene Hydroxylase; D001189:Aryl Hydrocarbon Hydroxylases