title: Low burst-promoting activity (BPA) production by cord mononuclear cells is due to functional immaturity of monocytes.
abstract: Cord blood mononuclear cells produced lower burst-promoting activity (BPA) than adult peripheral blood mononuclear cells when stimulated with phytohemagglutinin (PHA). In order to examine the cellular basis of the low production of BPA by PHA-stimulated cord blood mononuclear cells in the context of the functional immaturity of T cells or monocytes, we studied BPA production by T cells or monocytes from cord blood and adult peripheral blood. Cord T cells produced as much BPA as adult T cells. Monocytes themselves did not produce significant BPA at the concentration used in this experiment (1 x 10(5)/ml). BPA production by adult T cells was significantly enhanced by the presence of autologous monocytes. BPA production by cord T cells was also enhanced by the presence of adult monocytes but not by that of cord monocytes. Cord monocytes did not enhance BPA production by adult T cells either. These results indicate that cord monocytes are primarily responsible for the low BPA production by PHA-stimulated cord mononuclear cells.
authors.affiliation: Department of Pediatrics, Kyoto University, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Iwai
authors.forename: M
authors.initials: M
authors.lastname: Mayumi
authors.forename: K M
authors.initials: KM
authors.lastname: Kim
authors.forename: M
authors.initials: M
authors.lastname: Tanaka
authors.forename: H
authors.initials: H
authors.lastname: Mikawa
mesh_terms: D004920:Erythropoiesis; D005312:Fetal Blood / Q000166:cytology*; D006801:Humans; D007231:Infant, Newborn; D007377:Interleukin-3 / Q000096:biosynthesis*; D009000:Monocytes / Q000378:metabolism* / Q000502:physiology; D013601:T-Lymphocytes / Q000378:metabolism* / Q000502:physiology
chemical_list: D007377:Interleukin-3