[Cytometric method of determining the parameters of the interaction of synthetic immunostimulants with the lymphocytic surface].
The cytofluorimetric method was suggested for the determination of parameters of polymer adsorption on the cellular surface. Multiple equilibrium in the homogeneous cellular population with N independent binding sites and in the heterogeneous population of cells with the Poisson distribution of adsorption sites was analysed. A possibility was shown of cytofluorimetric drawing of the adsorption isotherm using mean fluorescence intensity of cells in the equilibrium suspension. The equilibrium association constant and the mean number of binding sites were estimated for adsorption of poly-L-lysin on the surface of human myelogenous leukemic cells (cellular line K-252).
Shcherbukhin
V V
VV
Zasypkin
D V
DV
Ataullakhanov
F I
FI
Skuĭbin
B G
BG
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000327:Adsorption; D001665:Binding Sites / Q000187:drug effects; D002460:Cell Line; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D007951:Leukemia, Myeloid / Q000378:metabolism / Q000473:pathology; D008024:Ligands; D008214:Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D008433:Mathematics; D008954:Models, Biological; D011107:Polylysine / Q000494:pharmacology; D013499:Surface Properties; D014407:Tumor Cells, Cultured
D000276:Adjuvants, Immunologic; D008024:Ligands; D011107:Polylysine
Tsitometricheskiĭ metod oprdeleniia parametrov vazimodeĭstviia sinteticheskikh immunostimuliatorov s poverkhnost'iu limfotsitov.