Epidermal growth factor binding in rat uterus during the peri-implantation period.
The profile of epidermal growth factor (EGF) binding to uterine membranes of rats on Day 1 through Day 7 of pregnancy was studied. The binding was lowest on Day 1 and increased gradually through the pre- and postimplantation periods. Binding affinity of the Day 7 uterine membranes was considerably higher than that of the Day 1. Apparent affinity constants (Ka) of Day 1 and Day 7 membranes were 0.29 X 10(-8) M and 1.03 X 10(-8) M respectively. To our knowledge, this is the first report of the modulation of EGF binding to uterine membranes by progesterone-estrogen interaction during early pregnancy.
Chakraborty
C
C
Department of Gynecology & Obstetrics, University of Kansas Medical Center, Kansas City 66103.
Tawfik
O W
OW
Dey
S K
SK
D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D010064:Embryo Implantation; D004815:Epidermal Growth Factor / Q000378:metabolism*; D066246:ErbB Receptors / Q000378:metabolism*; D005260:Female; D005865:Gestational Age; D007700:Kinetics; D011247:Pregnancy; D011270:Pregnancy, Animal; D011374:Progesterone / Q000502:physiology; D011869:Radioligand Assay; D051381:Rats; D014599:Uterus / Q000378:metabolism*
D011374:Progesterone; D004815:Epidermal Growth Factor; D066246:ErbB Receptors