Generation of multiple troponin T isoforms is a common feature of the muscles in various chordate animals.
1. Troponin T (TNT) expressed in various vertebrate skeletal and ascidian smooth muscles was examined by two-dimensional electrophoresis in combination with immunoblotting. 2. A monoclonal anti-TNT antibody, NT-302, exhibited binding ability to various TNT variants in the vertebrate and protochordate animals. 3. TNT isoform pattern differed among the animals, but the existence of multiple TNT isoforms in a single muscle tissue was the general feature of all the animals examined.
Ohshima
S
S
Department of Biology, Faculty of Science, Chiba University, Japan.
Komiya
T
T
Takeuchi
K
K
Endo
T
T
Obinata
T
T
D000818:Animals; D000911:Antibodies, Monoclonal; D002816:Chordata, Nonvertebrate / Q000235:genetics / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D014644:Genetic Variation; D015151:Immunoblotting; D009130:Muscle, Smooth / Q000032:analysis; D009132:Muscles / Q000032:analysis*; D014336:Troponin / Q000276:immunology / Q000302:isolation & purification*
D000911:Antibodies, Monoclonal; D014336:Troponin