Structural analysis by fast-atom-bombardment mass spectrometry of the mixture of alditols derived from the O-linked oligosaccharides of murine glycophorins.
The O-glycosylically linked oligosaccharides from glycophorins of BALB/c mouse erythrocytes were released as a mixture of alditol derivatives on reductive beta-elimination. A new approach, based on periodate oxidation in combination with f.a.b.-m.s., was used to elucidate the structure of one of the branched derivatives in the mixture. Evidence for the anomeric configuration was obtained by 500-MHz 1H-n.m.r. spectroscopy. The following structures were found: (Formula: see text).
Krotkiewski
H
H
Department of Immunochemistry, Polish Academy of Sciences, Wrocław.
Lisowska
E
E
Angel
A S
AS
Nilsson
B
B
D000818:Animals; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D004912:Erythrocytes / Q000032:analysis; D006020:Glycopeptides / Q000302:isolation & purification; D006021:Glycophorins / Q000302:isolation & purification; D007202:Indicators and Reagents; D013058:Mass Spectrometry / Q000379:methods; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000302:isolation & purification; D012795:Sialoglycoproteins / Q000302:isolation & purification; D013402:Sugar Alcohols / Q000032:analysis*
D006020:Glycopeptides; D006021:Glycophorins; D007202:Indicators and Reagents; D009844:Oligosaccharides; D012795:Sialoglycoproteins; D013402:Sugar Alcohols