Serology-based potency test for inactivated Newcastle disease vaccines.
Twenty-seven inactivated oil-emulsion Newcastle disease vaccines were tested for potency in chickens. Serum samples from groups given 1/50 dose of vaccine were examined by the haemagglutination inhibition (HI) test and the indirect enzyme-linked immunosorbent assay (ELISA). Good correlations were observed between potency and HI titres and between potency and ELISA absorbance. It is recommended that a serology-based potency test replaces the challenge test, reserving challenge only for those batches of vaccines where a clear pass is not indicated.
Goddard
R D
RD
Central Veterinary Laboratory, New Haw, Weybridge, Surrey, UK.
Nicholas
R A
RA
Luff
P R
PR
D000818:Animals; D002645:Chickens; D004306:Dose-Response Relationship, Immunologic; D004797:Enzyme-Linked Immunosorbent Assay; D006385:Hemagglutination Inhibition Tests; D007106:Immune Sera; D009521:Newcastle Disease / Q000517:prevention & control*; D014611:Vaccination; D015164:Vaccines, Inactivated / Q000592:standards*; D014765:Viral Vaccines / Q000592:standards*
D007106:Immune Sera; D015164:Vaccines, Inactivated; D014765:Viral Vaccines