Analysis of the provirus genome integrated in T cell lines established from the cerebrospinal fluid of patients with human T lymphotropic virus type I-associated myelopathy (HAM).
T cell lines were established from the cerebrospinal fluid (CSF) lymphocytes of three patients with human T lymphotropic virus type I (HTLV-I)-associated myelopathy (HAM). To elucidate the possible changes of the provirus nucleotide sequences integrated in HAM-derived T cell line cells, DNA from these cells was digested with PstI, which cleaved the provirus genome of HTLV-I at several sites, and three common bands were detected by Southern blot analysis in all cases. These bands were identical in size to those detected in T cell lines established from peripheral blood lymphocytes of adult T cell leukemia (ATL) patients. These results were confirmed with restriction enzymes HindIII and BamHI. These findings suggest that the provirus genome detected in T cell lines derived from CSF of HAM patients is identical to HTLV-I.
Nishimura
M
M
Department of Neurology, Faculty of Medicine, Kyoto University, Japan.
Adachi
A
A
Maeda
M
M
Akiguchi
I
I
Fujita
M
M
Kameyama
M
M
Ishimoto
A
A
D000328:Adult; D001773:Blood Cells / Q000502:physiology; D015139:Blotting, Southern; D015153:Blotting, Western; D002460:Cell Line; D002555:Cerebrospinal Fluid / Q000166:cytology*; D005260:Female; D005814:Genes, Viral; D015490:HTLV-I Infections / Q000150:complications*; D006801:Humans; D015458:Leukemia, T-Cell / Q000235:genetics / Q000382:microbiology; D008214:Lymphocytes / Q000502:physiology; D008297:Male; D008875:Middle Aged; D015493:Paraparesis, Tropical Spastic / Q000235:genetics / Q000382:microbiology*; D011533:Proviruses / Q000235:genetics*; D013118:Spinal Cord Diseases / Q000209:etiology* / Q000235:genetics / Q000382:microbiology; D013601:T-Lymphocytes / Q000502:physiology*