title: Further evidence for H-2-unrestricted induction of minor histocompatibility antigens-specific T cell immunity in vivo.
abstract: Antigenic requirements for inducing minor histocompatibility antigens (MIHA)-specific T cell immunity for second set rejection (SSR) of a MIHA-allogeneic tumor were studied. An intravenous injection of surprisingly small numbers (10(4)-10(5] of live allogenetic spleen cells (SC) effectively primed mice for SSR of the allogeneic tumor, and this immunity was developed as early as 2-3 days after injection of the SC. In contrast, sonication-disrupted allogeneic SC, which should be readily processed by host antigen presenting cells (APC), were not active as immunogens, even at a dose 1000 times higher than the minimum effective dose of live SC. The possibility that host APC preferentially receive MIHA antigens shed by live allogeneic SC for T cell activation was ruled out. These results demonstrated that antigen processing via conventional pathways is very little involved in the mechanism of T cell activation. Under such restricted experimental conditions, the induction phase but not the effector phase of the MIHA-specific T cell immunity was shown to be H-2-unrestricted.
authors.affiliation: Department of Immunology, Nagoya University School of Medicine, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Mizoguchi
authors.forename: K
authors.initials: K
authors.lastname: Isobe
authors.forename: T
authors.initials: T
authors.lastname: Yoshida
authors.forename: T
authors.initials: T
authors.lastname: Iwamoto
authors.forename: T
authors.initials: T
authors.lastname: Hasegawa
authors.forename: L
authors.initials: L
authors.lastname: Ding
authors.forename: S M
authors.initials: SM
authors.lastname: Rahman
authors.forename: T
authors.initials: T
authors.lastname: Miyata
authors.forename: F
authors.initials: F
authors.lastname: Nagase
authors.forename: K
authors.initials: K
authors.lastname: Shimokata
mesh_terms: D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology; D006084:Graft Rejection; D006183:H-2 Antigens / Q000276:immunology*; D006649:Histocompatibility Antigens / Q000276:immunology*; D051379:Mice; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000276:immunology; D013154:Spleen / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
chemical_list: D006183:H-2 Antigens; D006649:Histocompatibility Antigens