title: Effects of proprietary oral rinses containing chlorhexidine, hexetidine and benzydamine on the proliferation of human buccal epithelial cells in culture.
abstract: Cell cultures were established from small samples of buccal tissue, using a 3T3 fibroblast feeder-layer technique. After exposure to increasing dilutions of three proprietary oral rinses for 22 h or 2 h, the effects upon cell proliferation were studied by measurement of [3H]-thymidine incorporation into cellular DNA. Cell membrane damage was assessed by measurement of lactate dehydrogenase content. Cultures exposed to hexetidine-containing or chlorhexidine-containing rinses for 22 h at dilutions of 250-fold or lower showed almost complete inhibition of [3H]-thymidine incorporation. Cultures treated with benzydamine-containing rinse at the same dilutions showed no significant inhibition of incorporation. Exposure to the same dilutions of hexetidine- and chlorhexidine-containing rinses for 2 h resulted in 65% and 20% inhibition of incorporation, respectively. Lactate dehydrogenase content decreased to negligible levels after exposure to the rinse containing hexetidine at a 250-fold dilution, but was unaffected by the other two rinses. Thus dividing buccal epithelial cells in vitro may be adversely affected by exposure to certain commercial oral rinses.
authors.affiliation: Laing Laboratory for Burn Injury Investigation, Odstock Hosptial, Salisbury, England.
authors.forename: V
authors.initials: V
authors.lastname: Shakespeare
authors.forename: P G
authors.initials: PG
authors.lastname: Shakespeare
authors.forename: B T
authors.initials: BT
authors.lastname: Evans
mesh_terms: D001591:Benzydamine / Q000494:pharmacology; D002478:Cells, Cultured; D002610:Cheek; D002710:Chlorhexidine / Q000494:pharmacology; D004247:DNA / Q000096:biosynthesis; D004848:Epithelium / Q000187:drug effects / Q000201:enzymology; D006590:Hexetidine / Q000494:pharmacology; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D008938:Mitosis / Q000187:drug effects; D009061:Mouth Mucosa / Q000166:cytology*; D009067:Mouthwashes / Q000494:pharmacology*
chemical_list: D009067:Mouthwashes; D001591:Benzydamine; D006590:Hexetidine; D004247:DNA; D007770:L-Lactate Dehydrogenase; D002710:Chlorhexidine