title: Trimethylamine oxidation in liver tissue is not catalyzed by a molybdenum cofactor-dependent enzyme.
abstract: The oxidation of trimethylamine to trimethylamine N-oxide in animals is catalyzed by an enzyme which has not yet been fully characterized. The discovery that a bacterial enzyme catalyzing the reverse reaction, the reduction of trimethylamine N-oxide to trimethylamine, utilizes the molybdenum cofactor to carry out this function raised the possibility that trimethylamine oxidation may also be dependent on this cofactor. It was found, however, that liver tissue from tungsten-treated rats contained normal levels of trimethylamine oxidase. In addition, analysis of a urine sample from a patient with trimethylamine oxidase deficiency revealed the presence of normal levels of urothione, the degradation product of the molybdenum cofactor. These results suggest that trimethylamine oxidase is not a molybdoenzyme and that oxidation of trimethylamine proceeds by a mechanism which differs considerably from a simple reversal of trimethylamine N-oxide reduction.
authors.affiliation: Department of Biochemistry, Duke University Medical Center, Durham, NC 27710.
authors.forename: J L
authors.initials: JL
authors.lastname: Johnson
mesh_terms: D000818:Animals; D003067:Coenzymes / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008667:Metalloproteins / Q000378:metabolism*; D008744:Methylamines / Q000378:metabolism*; D008982:Molybdenum / Q000378:metabolism; D000090006:Molybdenum Cofactors; D010084:Oxidation-Reduction; D050862:Oxidoreductases Acting on Sulfur Group Donors / Q000378:metabolism; D010089:Oxidoreductases, N-Demethylating / Q000378:metabolism; D011621:Pteridines / Q000378:metabolism*; D051381:Rats; D012016:Reference Values; D014414:Tungsten / Q000494:pharmacology
chemical_list: D003067:Coenzymes; D008667:Metalloproteins; D008744:Methylamines; D008982:Molybdenum; D000090006:Molybdenum Cofactors; D050862:Oxidoreductases Acting on Sulfur Group Donors; D010089:Oxidoreductases, N-Demethylating; D011621:Pteridines; D014414:Tungsten; C022261:molybdopterin; C023186:trimethylamine dehydrogenase; C023336:trimethylamine