Fluorometric determination of benzylideneacetone in fragrance products by liquid chromatography with post-column derivatization.
A method is described for the liquid chromatographic (LC)-fluorometric determination of benzylideneacetone in fragrance products. Benzylideneacetone is first separated from other fragrance ingredients by LC and then reacted post-column with a methanolic solution of isonicotinic acid hydrazide and aluminum nitrate. The reactants are maintained at 65 degrees C for about 1.5 min to quantitatively form the fluorescent isonicotinoyl hydrazone derivative of benzylideneacetone. The aluminum ion forms a complex with the hydrazone to enhance the fluorescence of the derivative. The amount of benzylideneacetone is determined by measuring the intensity of the fluorescence emitted by the hydrazone derivative and comparing that value with those obtained for derivatized standards. Recovery studies were conducted by spiking commercial fragrances with benzylideneacetone at concentrations of 0.01, 0.05, and 0.1% (w/v). Recoveries ranged from 98 to 104% with a mean recovery of 100.2% and a standard deviation of 2.4%.
Yates
R L
RL
Food and Drug Administration, Division of Colors and Cosmetics, Washington, DC 20204.
Wenninger
J A
JA
D002074:Butanones / Q000032:analysis*; D007202:Indicators and Reagents; D009821:Oils / Q000032:analysis; D010476:Perfume / Q000032:analysis*; D012997:Solvents; D013050:Spectrometry, Fluorescence
D002074:Butanones; D007202:Indicators and Reagents; D009821:Oils; D010476:Perfume; D012997:Solvents; C025383:benzylideneacetone