title: The myeloid blood cell differentiation-inducing protein MGI-2A is interleukin-6.
abstract: The mouse myeloid blood cell differentiation-inducing protein, macrophage and granulocyte inducer, type 2A (MGI-2A), was purified, and the amino acid sequence of a CNBr cleavage peptide (22 residues) was determined. This amino acid sequence is identical to the sequence found in positions 73 to 94 of mouse interleukin-6 (IL-6). Recombinant mouse IL-6 protein induces differentiation of mouse myeloid leukemic cells that are induced to differentiation by MGI-2, and monoclonal antimouse-MGI-2 antibody, which neutralizes MGI-2, also completely neutralizes this IL-6-induced differentiation. These results show that the major type of mouse myeloid differentiation-inducing protein (MGI-2A) and IL-6 are very similar and most likely identical proteins. Recombinant human IL-6 (also called interferon-beta 2 or B-cell differentiation factor), which shows only a 41% similarity to mouse IL-6, has 11 identical amino acid residues out of the 22 in the mouse MGI-2A peptide and also induces differentiation of the same myeloid leukemic cells.
authors.affiliation: Department of Genetics, Weizmann Institute of Science, Rehovot, Israel.
authors.forename: Y
authors.initials: Y
authors.lastname: Shabo
authors.forename: J
authors.initials: J
authors.lastname: Lotem
authors.forename: M
authors.initials: M
authors.lastname: Rubinstein
authors.forename: M
authors.initials: M
authors.lastname: Revel
authors.forename: S C
authors.initials: SC
authors.lastname: Clark
authors.forename: S F
authors.initials: SF
authors.lastname: Wolf
authors.forename: R
authors.initials: R
authors.lastname: Kamen
authors.forename: L
authors.initials: L
authors.lastname: Sachs
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D015850:Interleukin-6; D007378:Interleukins / Q000494:pharmacology*; D007951:Leukemia, Myeloid / Q000473:pathology*; D051379:Mice; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000473:pathology
chemical_list: D015850:Interleukin-6; D007378:Interleukins; D010446:Peptide Fragments