title: A specific ELISA method for determining chloroquine in urine or dried blood spots.
abstract: Reported is an enzyme-linked immunosorbent assay (ELISA) that has been optimized and validated for the determination of chloroquine in urine or dried blood spots. The assay employs antisera raised in sheep to a chloroquine derivative conjugated to keyhole limpet haemocyanin and chloroquine conjugated to porcine thyroglobulin adsorbed onto the wells of a microtitration plate. The competitive binding of the antiserum to the wells was monitored using an alkaline-phosphatase-conjugated second antibody and a specific substrate. The assay exhibits no cross-reactivity with known chloroquine metabolites, other antimalarials, and commonly used drugs. The method was used to determine chloroquine in dried blood spot extracts and urine from a patient who was receiving a prescribed prophylactic chloroquine regimen. The drug was detected in the urine for 17 weeks and in the dried blood spots for 4 weeks after termination of the therapy.
authors.forename: V
authors.initials: V
authors.lastname: Rowell
authors.forename: F J
authors.initials: FJ
authors.lastname: Rowell
authors.forename: A
authors.initials: A
authors.lastname: Baker
authors.forename: D
authors.initials: D
authors.lastname: Laurie
authors.forename: A M
authors.initials: AM
authors.lastname: Sidki
mesh_terms: D000328:Adult; D000906:Antibodies / Q000032:analysis; D002738:Chloroquine / Q000097:blood / Q000276:immunology / Q000652:urine*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D007106:Immune Sera / Q000302:isolation & purification; D008297:Male
chemical_list: D000906:Antibodies; D007106:Immune Sera; D002738:Chloroquine