1H-NMR relaxation study of the liposome--nuclei interaction.
Phospholipidic liposomes can influence DNA template availability for RNA synthesis when interacting with isolated nuclei by enhancing (when the phospholipids are negatively charged) or by inhibiting (when they are neutral) trascriptional activity. The analysis of the 1H-NMR relaxation behaviour of isolated nuclei, of Small Unilamellar Vesicles (SUV) and of the SUV-Nuclei system has been undertaken to elucidate the mechanism of the interaction between the nuclear core and the phospholipids.
Boicelli
A
A
IRCCS San Raffaele Milano, Italy.
Caramelli
E
E
Baldassarri
A M
AM
Giuliani
A M
AM
Capitani
S
S
Billi
A M
AM
Manzoli
F A
FA
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism* / Q000648:ultrastructure; D005614:Freeze Fracturing; D006859:Hydrogen; D008081:Liposomes; D008099:Liver / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008297:Male; D010713:Phosphatidylcholines / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D008081:Liposomes; D010713:Phosphatidylcholines; D006859:Hydrogen