title: Expression of the parasite protein Pc90 in plasma membranes of erythrocytes infected with Plasmodium chabaudi.
abstract: Erythrocytes infected with the malaria parasite Plasmodium chabaudi contain the neo-protein Pc90 in their plasma membrane. We investigate origin, membrane disposition, and intraerythrocytic traffic of this Pc90. Metabolic labeling of P.-infected erythrocytes, combined with cell fractionation as well as Western blot analysis and immunoprecipitation using a Pc90-recognizing monoclonal antibody, show that Pc90 is synthesized by early to mid trophozoites and is transported without any apparent processing steps to the erythrocyte membrane. Based upon the inaccessibility of Pc90 from the outside in intact erythrocytes and the water solubility of membrane-associated Pc90, it is concluded that Pc90 is localized on the cytoplasmic face of the host erythrocyte membrane. Immunoelectron microscopy using a Pc90-specific monoclonal antibody and the occurrence of soluble Pc90 in host cell cytosol indicate that the Pc90 is transported in both a 'vesicle-bound' and a 'free' form through the erythrocyte cytoplasm.
authors.affiliation: Division of Parasitology, University of Düsseldorf, Federal Republic of Germany.
authors.forename: F
authors.initials: F
authors.lastname: Wunderlich
authors.forename: M
authors.initials: M
authors.lastname: Helwig
authors.forename: G
authors.initials: G
authors.lastname: Schillinger
authors.forename: V
authors.initials: V
authors.lastname: Speth
authors.forename: M F
authors.initials: MF
authors.lastname: Wiser
mesh_terms: D000818:Animals; D004910:Erythrocyte Membrane / Q000276:immunology / Q000378:metabolism / Q000469:parasitology*; D006790:Host-Parasite Interactions; D051379:Mice; D010961:Plasmodium / Q000276:immunology* / Q000302:isolation & purification