Hormone conjugated with antibody to CD3 mediates cytotoxic T cell lysis of human melanoma cells.
Cytotoxic T lymphocytes can be activated by antibodies to their antigen-specific receptor complex (TCR-CD3) to destroy target cells, regardless of the specificity of the cytotoxic T cells. A novel hormone-antibody conjugate, consisting of an analog of melanocyte-stimulating hormone chemically coupled to a monoclonal antibody to CD3, the invariant component of the T cell receptor complex, was used to target human melanoma cells for destruction by human cytotoxic T lymphocytes that bear no specificity for the tumor cells. As targeting components of such anti-CD3 conjugates, hormones or growth factors are expected to prove more effective than antibodies to tumor-associated antigens in focusing the destructive activity of cytotoxic T cells on tumor target cells.
Liu
M A
MA
Center for Cancer Research, Massachusetts Institute of Technology, Cambridge 02139.
Nussbaum
S R
SR
Eisen
H N
HN
D000911:Antibodies, Monoclonal / Q000627:therapeutic use*; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology; D000951:Antigens, Neoplasm / Q000276:immunology; D003602:Cytotoxicity, Immunologic; D006801:Humans; D008213:Lymphocyte Activation; D009074:Melanocyte-Stimulating Hormones / Q000031:analogs & derivatives* / Q000378:metabolism / Q000627:therapeutic use; D008545:Melanoma / Q000628:therapy*; D011948:Receptors, Antigen, T-Cell / Q000276:immunology*; D011961:Receptors, Fc / Q000276:immunology; D011979:Receptors, Pituitary Hormone / Q000378:metabolism; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D000951:Antigens, Neoplasm; D011948:Receptors, Antigen, T-Cell; D011961:Receptors, Fc; D011979:Receptors, Pituitary Hormone; C029446:MSH receptor; D009074:Melanocyte-Stimulating Hormones