An enzyme-linked immunoassay for thromboxane B2 and 6-oxo-PGF1 alpha using peroxidase as label.
A sensitive and specific enzyme immunoassay was developed for TXB2 and 6-oxo-PGF1 alpha, stable degradation products of TXA2 and prostacyclin. The hapten molecule was labeled with pure peroxidase. After competitive binding to antibody between enzyme-labeled and free prostanoids the immunoreactive complex was precipitated by double antibody technique. The enzyme activity of the precipitate was determined spectrophotometrically. The procedure allowed a determination of TXB2 and 6-oxo-PGF1 alpha in the range of 3-200 pg (0.008-0.53 pmol) and 10-1000 pg (0.028-2.8 pmol) respectively.
Giessler
C
C
Department of Pharmacology and Toxicology, Martin Luther University Halle-Wittenberg, GDR.
Panse
M
M
Mentz
P
P
Hellthaler
G
G
D015121:6-Ketoprostaglandin F1 alpha / Q000032:analysis*; D000818:Animals; D000936:Antigen-Antibody Complex; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D008832:Microchemistry; D011817:Rabbits; D013929:Thromboxane B2 / Q000032:analysis*
D000936:Antigen-Antibody Complex; D013929:Thromboxane B2; D015121:6-Ketoprostaglandin F1 alpha