Indirect tissue electrophoresis: a new method for analyzing solid tissue protein.
1. The eye lens core (nucleus) has been a valuable source of molecular biologic information. 2. In these studies, lens nuclei are usually homogenized so that any protein information related to anatomical subdivisions, or layers, of the nucleus is lost. 3. The present report is of a new method, indirect tissue electrophoresis (ITE), which, when applied to fish lens nuclei, permitted (a) automatic correlation of protein information with anatomic layer, (b) production of large, clear electrophoretic patterns even from small tissue samples and (c) detection of more proteins than in liquid extracts of homogenized tissues. 4. ITE seems potentially applicable to a variety of solid tissues.
Smith
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AC
Laboratory Service, Veterans Administration Medical Center, Bay Pines, FL 33504.
D000818:Animals; D003459:Crystallins / Q000032:analysis*; D004586:Electrophoresis / Q000379:methods*; D005399:Fishes; D007907:Lens Nucleus, Crystalline / Q000032:analysis
D003459:Crystallins