Biotinylated recombinant interleukin-2. A tool for research on the interleukin-2 receptor.
Recombinant interleukin-2 was biotinylated using a N-hydroxyl-succinimidyl [3H]biotin ester. The biotinylated lymphokine retained full binding and growth-promoting activities when assayed on the interleukin-2-dependent murine cell line HT-2. In preliminary studies, biotinylated interleukin-2 was used in conjunction with immunogold staining to demonstrate cell surface interleukin-2 receptors using both light and electron microscopy techniques. In addition, with rabbit anti-biotin antibodies, biotin-interleukin-2 was able to precipitate the 55 kDa IL-2 receptor from murine HT-2 cells. Thus, biotin-interleukin-2 represents a useful, non-radioactive tool for studying the structure and function of the interleukin-2 receptor.
Foxwell
B M
BM
Preclinical Research, Sandoz Ltd., Basel, Switzerland.
Taylor
D
D
Greiner
B
B
Mihatsch
M J
MJ
Olivieri
V
V
Ryffel
B
B
D000818:Animals; D001710:Biotin; D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel; D007150:Immunohistochemistry / Q000379:methods; D007376:Interleukin-2 / Q000378:metabolism; D008565:Membrane Proteins / Q000302:isolation & purification / Q000378:metabolism; D051379:Mice; D011233:Precipitin Tests / Q000379:methods; D015375:Receptors, Interleukin-2 / Q000032:analysis / Q000302:isolation & purification*; D011994:Recombinant Proteins / Q000378:metabolism; D012967:Sodium Dodecyl Sulfate; D013602:T-Lymphocytes, Cytotoxic / Q000032:analysis / Q000648:ultrastructure
D007376:Interleukin-2; D008565:Membrane Proteins; D015375:Receptors, Interleukin-2; D011994:Recombinant Proteins; D012967:Sodium Dodecyl Sulfate; D001710:Biotin