Quantitative analysis of serum methohexital by GLC using capillary column and nitrogen-selective detection.
A gas chromatographic method for routine quantitation of methohexital in plasma samples is reported. One-step extraction in organic phase, the use of a fused silica capillary column, and nitrogen-selective detection permit simple, precise, and sensitive determination of methohexital in plasma. A linear relationship is described between peak height ratio and methohexital concentrations ranging from 0.125 to 50.0 micrograms/ml (r = 0.998). The sensitivity limit of the assay was 6 ng/ml in plasma. No interfering peak was observed with numerous other drugs. The procedure was successfully applied to the determination of pharmacokinetic parameters of methohexital after IV administration or continuous infusion in a child and an adult.
Le Normand
Y
Y
Département de Pharmacologie, Faculté de Médecine, Nantes, France.
De Villepoix
C
C
Athouel
A
A
Kergueris
M F
MF
Bourin
M
M
Larousse
C
C
D055598:Chemical Phenomena; D002621:Chemistry; D002849:Chromatography, Gas; D006801:Humans; D008723:Methohexital / Q000097:blood* / Q000378:metabolism / Q000493:pharmacokinetics
D008723:Methohexital