The effect of interleukin-1, interleukin-6 and its interrelationship on the synthesis of serum amyloid A and C-reactive protein in primary cultures of adult human hepatocytes.
During the acute phase response, synthesis of C-reactive protein and serum amyloid A is increased. To investigate whether the enhanced synthesis of these proteins are due to stimulatory effect of inflammatory mediators such as interleukin-1 (IL-1) and interleukin-6 (IL-6) produced by macrophages and monocytes, primary cultures of adult human hepatocytes were exposed to recombinant (r)IL-1, rIL-6 or rIL-1 and monospecific anti rIL-6 antibodies in the presence of 1 microM dexamethasone. The findings indicate that rIL-1 and rIL-6 both stimulate the liver synthesis of C-reactive protein and serum amyloid A, however monospecific anti rIL-6 antibodies reduce the stimulatory effect of rIL-1 on the synthesis of these proteins. These findings suggest that IL-6 plays a key role in the stimulation of synthesis of serum amyloid A and C-reactive protein by the human liver cells.
Moshage
H J
HJ
Department of Medicine, St. Radboud University Hospital, Nijmegen, The Netherlands.
Roelofs
H M
HM
van Pelt
J F
JF
Hazenberg
B P
BP
van Leeuwen
M A
MA
Limburg
P C
PC
Aarden
L A
LA
Yap
S H
SH
D000293:Adolescent; D000328:Adult; D000418:Albumins / Q000096:biosynthesis; D002097:C-Reactive Protein / Q000096:biosynthesis*; D002478:Cells, Cultured; D003907:Dexamethasone / Q000494:pharmacology; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology; D007375:Interleukin-1 / Q000494:pharmacology*; D015850:Interleukin-6; D007378:Interleukins / Q000276:immunology / Q000494:pharmacology*; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D008875:Middle Aged; D011994:Recombinant Proteins / Q000276:immunology / Q000494:pharmacology; D000685:Serum Amyloid A Protein / Q000096:biosynthesis*
D000418:Albumins; D007106:Immune Sera; D007375:Interleukin-1; D015850:Interleukin-6; D007378:Interleukins; D011994:Recombinant Proteins; D000685:Serum Amyloid A Protein; D003907:Dexamethasone; D002097:C-Reactive Protein