The effects of IL-4 and IL-5 on the IgA response by murine Peyer's patch B cell subpopulations.
IL-5 has been shown to specifically enhance IgA secretion in LPS-stimulated splenic B cell cultures. Maximum enhancement of IgA in such cultures, however, requires IL-4 in addition to IL-5. Because the Peyer's patches (PP), compared with spleen and lymph nodes, are enriched for precursors of IgA-secreting cells, we tested whether IL-4 and IL-5 would have a more profound effect on IgA secretion by polyclonally stimulated PP cells than spleen cells. The combination of IL-4 and IL-5 causes a comparable enhancement of IgA secretion in both LPS-stimulated PP and splenic B cell cultures. The majority of IgA secreted in LPS-stimulated PP cell cultures is derived from the sIgA- population. Furthermore, the binding high level of peanut agglutinin, germinal center subpopulation of PP cells is essentially nonresponsive to LPS, even in the presence of lymphokines; the majority of secreted IgA in these cultures is derived from the binding low level of peanut agglutinin population. In contrast to LPS-stimulated cultures, PP B cells secrete considerably more IgA than splenic B cells when polyclonally stimulated by a clone of autoreactive T cells in the presence of IL-4 and IL-5. The majority of IgA made by T cell-stimulated PP cell cultures is derived from the sIgA+ population. In these cultures, sIgA- PP cells and spleen cells secrete comparable levels of IgA and other non-IgM isotypes suggesting that sIgA- PP B cells are similar to splenic B cells in their potential to switch to IgA. In T cell-stimulated cultures the majority of IgA as well as of all other isotypes is also derived from the nongerminal center, binding low level of peanut agglutinin population.
Lebman
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DA
DNAX Research Institute, Palo Alto, CA 94304-1104.
Coffman
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RL
D000818:Animals; D001402:B-Lymphocytes / Q000145:classification / Q000276:immunology / Q000378:metabolism*; D005260:Female; D007070:Immunoglobulin A / Q000096:biosynthesis*; D015847:Interleukin-4; D015848:Interleukin-5; D007378:Interleukins / Q000494:pharmacology*; D037102:Lectins / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D019887:Peanut Agglutinin; D010581:Peyer's Patches / Q000166:cytology*; D010641:Phenotype; D011947:Receptors, Antigen, B-Cell; D013154:Spleen / Q000166:cytology
D007070:Immunoglobulin A; D015848:Interleukin-5; D007378:Interleukins; D037102:Lectins; D008070:Lipopolysaccharides; D019887:Peanut Agglutinin; D011947:Receptors, Antigen, B-Cell; D015847:Interleukin-4