DNA lability induced by nimustine and ramustine in rat glioma cells.
The DNA labile sites induced by two nitrosoureas, nimustine (ACNU) and ramustine (MCNU) synthesised in Japan, have been examined in highly reiterated DNA sequences of rat glioma cells. Reiterated fragments of 167 and 203 base pairs (bp), obtained after Hind III and Hae III restriction endonuclease digestion of rat glioma cells DNA, were used as target DNA sequences to determine the labile sites. In vitro reaction with ACNU and MCNU resulted in scission products corresponding to the locations of guanine. Subsequent piperidine hydrolysis produced more frequent breaks of the phosphodiester bonds at guanine positions, thus forming alkali-labile sites.
Mineura
K
K
Neurosurgical Service, Akita University Hospital, Japan.
Fushimi
S
S
Itoh
Y
Y
Kowada
M
M
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001483:Base Sequence / Q000187:drug effects; D001932:Brain Neoplasms / Q000473:pathology; D002460:Cell Line; D004249:DNA Damage; D004273:DNA, Neoplasm / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D005910:Glioma / Q000473:pathology; D015376:Nimustine / Q000494:pharmacology*; D009607:Nitrosourea Compounds / Q000494:pharmacology*; D051381:Rats; D014407:Tumor Cells, Cultured / Q000187:drug effects*
D000970:Antineoplastic Agents; D004273:DNA, Neoplasm; D009607:Nitrosourea Compounds; D015376:Nimustine; C020766:ranimustine