title: [Isolation of 5-benzyl hydroxytryptophan enantiomers by using microbial aminoacylase].
abstract: The process of asymmetric deacylation of an acetyl derivative of 5-benzyl hydroxytryptophan racemate in the presence of native microbial aminoacylase was studied. The effect of pH, temperature, concentration of the enzyme and substrate were investigated. Conditions for preparation and isolation of individual amino acids were defined by optimization of N-Ac-DU-5-BOT hydrolysis.
authors.forename: L T
authors.initials: LT
authors.lastname: Kushashvili
authors.forename: A K
authors.initials: AK
authors.lastname: Zhuravlev
authors.forename: A G
authors.initials: AG
authors.lastname: Borodkin
authors.forename: G V
authors.initials: GV
authors.lastname: Popova
authors.forename: A D
authors.initials: AD
authors.lastname: Nekliudov
mesh_terms: D006916:5-Hydroxytryptophan / Q000031:analogs & derivatives* / Q000302:isolation & purification*; D000215:Acylation; D000581:Amidohydrolases / Q000494:pharmacology*; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007700:Kinetics; D012044:Regression Analysis; D012995:Solubility; D013237:Stereoisomerism; D013696:Temperature; D014364:Tryptophan / Q000031:analogs & derivatives* / Q000302:isolation & purification
chemical_list: C056145:5-benzyloxytryptophan; D014364:Tryptophan; D006916:5-Hydroxytryptophan; D000581:Amidohydrolases; C019672:aminoacylase I
vernacular_title: Poluchenie énantiomerov 5-benziloksitriptofana s pomoshch'iu mikrobnoĭ aminoatsilazy.