Determination of pterins in urine by high-performance liquid chromatography on C18 columns conditioned with cetyltrimethylammonium bromide.
A method is proposed for the determination of pterins in urine without clean-up by reversed-phase high-performance liquid chromatography on a C18 column previously conditioned with cetyltrimethylammonium bromide. Other endogenous compounds are retained in the column, preventing interference. Xanthopterin, neopterin, isoxanthopterin, monapterin, biopterin, 6-hydroxymethylpterin, pterin and lumazine can be determined. Retention and separation mechanisms are discussed.
Montalvo
B C
BC
Facultad de Ciencias Quimicas, Departamento de Quimica Analítica, Universidad Complutense de Madrid, Spain.
Villar
C I
CI
Hornillos
R C
RC
Diez
L P
LP
D000077286:Cetrimonium; D002593:Cetrimonium Compounds; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D011622:Pterins / Q000652:urine*; D013050:Spectrometry, Fluorescence
D002593:Cetrimonium Compounds; D007202:Indicators and Reagents; D011622:Pterins; D000077286:Cetrimonium