title: Granulocyte precursors are the principal cells in bone marrow that stimulate allospecific cytolytic T-lymphocyte responses.
abstract: Mouse bone marrow cells were fractionated and enriched for functional activity as stimulators of allospecific cytolytic T-lymphocyte (CTL) responses in vitro. The relevant stimulator cells were enriched sequentially in the low-density fraction of bone marrow, its 2-hr adherent and 18-hr non-adherent fractions and in the FcR-negative fraction of 18-hr non-adherent cells. The functionally enriched cell population contained over 90% granulocyte precursors by ultrastructural analysis. The results indicate that granulocyte precursors are the principal cells in bone marrow that stimulate alloreactive T-cell responses.
authors.affiliation: Mount Sinai Hospital Research Institute, University of Toronto, Ontario, Canada.
authors.forename: A A
authors.initials: AA
authors.lastname: Czitrom
authors.forename: T S
authors.initials: TS
authors.lastname: Axelrod
authors.forename: B
authors.initials: B
authors.lastname: Fernandes
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000276:immunology*; D002448:Cell Adhesion; D002469:Cell Separation; D002499:Centrifugation, Density Gradient; D006098:Granulocytes / Q000276:immunology* / Q000648:ultrastructure; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D011961:Receptors, Fc / Q000032:analysis; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
chemical_list: D011961:Receptors, Fc