title: The T cell receptor V alpha 3 gene segment is associated with reactivity to p-azobenzenearsonate.
abstract: The murine T cell receptor V alpha 3 gene segment is associated with reactivity to p-azobenzenearsonate, as indicated by several independent lines of evidence. First, three out of four arsonate-reactive T cell clones tested (two I-Ad-and one I-Ak-restricted) utilized V alpha 3. Second, bulk splenic cultures enriched for arsonate/H-2d and arsonate/H-2k responsive T cells showed increased expression of V alpha 3 mRNA. Third, a V alpha 3-containing alpha chain (Ar-5: arsonate/I-Ad) transferred arsonate responsiveness to an appropriate recipient T cell (O3: ovalbumin/I-Ad). Fourth, an independently derived V alpha 3-expressing T cell clone (2C: alloreactive to Ld) showed a response to arsonate/Ld. Thus, a V alpha 3 gene segment, in conjunction with at least two different J alpha segments (J alpha 20'(Ar-5) and J alpha pHDS58(2C)) and at least three different beta chains (V beta 2(Ar-5), V beta 6(O3), and V beta 8(2C], confers reactivity to arsonate in association with at least three different MHC proteins (I-Ad, I-Ak, and Ld). We suggest that V alpha 3 encodes a protein sequence with a binding site for the arsonate hapten.
authors.affiliation: Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts.
authors.forename: K N
authors.initials: KN
authors.lastname: Tan
authors.forename: B M
authors.initials: BM
authors.lastname: Datlof
authors.forename: J A
authors.initials: JA
authors.lastname: Gilmore
authors.forename: A C
authors.initials: AC
authors.lastname: Kronman
authors.forename: J H
authors.initials: JH
authors.lastname: Lee
authors.forename: A M
authors.initials: AM
authors.lastname: Maxam
authors.forename: A
authors.initials: A
authors.lastname: Rao
mesh_terms: D000818:Animals; D001391:Azo Compounds / Q000276:immunology*; D001483:Base Sequence; D002999:Clone Cells; D004247:DNA / Q000235:genetics; D005796:Genes; D006825:Hybridomas; D051379:Mice; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000235:genetics; D011948:Receptors, Antigen, T-Cell / Q000235:genetics* / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D010132:p-Azobenzenearsonate / Q000276:immunology*
chemical_list: D001391:Azo Compounds; D012333:RNA, Messenger; D011948:Receptors, Antigen, T-Cell; D010132:p-Azobenzenearsonate; D004247:DNA