Internally standardized method for the determination of nalbuphine in human plasma by means of high performance liquid chromatography with electrochemical coulometric detection.
A high performance liquid chromatographic method with internal analogue standardization and electrochemical detection for the determination of nalbuphine in human plasma is described. Using 3 ml plasma the lower limit of detection is below 50 pg/ml, during the routine assay the limit of determination can be fixed at about 250-300 pg/ml. The calibration curve is linear in the range between 0.163-65 ng/l, the recovery rate from plasma exceeds 80%. The method was successfully applied in several pharmacokinetic studies.
Wetzelsberger
N
N
Institut für klinische Pharmakologie Bobenheim, Grünstadt, Fed. Rep. of Germany.
Lücker
P W
PW
Erking
W
W
D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D006801:Humans; D007202:Indicators and Reagents; D009019:Morphinans / Q000097:blood*; D009266:Nalbuphine / Q000097:blood* / Q000493:pharmacokinetics; D009271:Naltrexone / Q000494:pharmacology; D011786:Quality Control; D012015:Reference Standards
D007202:Indicators and Reagents; D009019:Morphinans; D009271:Naltrexone; D009266:Nalbuphine