Enhanced discrimination in the partition of proteins by aqueous polymer two-phase systems.
Aqueous polymer two-phase systems containing dextran T-500 and PEG 4000 can be prepared which are biphasic below 18 degrees C and monophasic at higher temperatures. Both liganded and unliganded forms of glutamate dehydrogenase and troponin, which have similar partition coefficients if the protein is added to a two-phase system at 4 degrees C, have widely differing partition coefficients if added to the same system in the monophasic state at 20 degrees C and subsequently cooled to 4 degrees C.
Behizad
M
M
Department of Biochemistry, University of Sheffield, U.K.
Treffry
T
T
D001671:Biochemistry / Q000379:methods*; D005969:Glutamate Dehydrogenase / Q000302:isolation & purification; D011108:Polymers
D011108:Polymers; D005969:Glutamate Dehydrogenase