title: Multiple pathways of inositol polyphosphate metabolism in angiotensin-stimulated adrenal glomerulosa cells.
abstract: The production and metabolism of inositol 1,4,5-trisphosphate (Ins-1,4,5-P3) and other inositol polyphosphates was studied in cultured bovine adrenal glomerulosa cells prelabeled for 24 h with [3H]inositol. During stimulation with angiotensin II, Ins-1,4,5-P3 increased to a peak of 15-fold above basal within 10 s, followed by a second phase of continuous increase over the next 30 min. Ins-1,4,5-P3 formed during agonist stimulation was rapidly metabolized by two distinct pathways. The more direct metabolic route was via degradation by sequential dephosphorylations to form inositol 1,4-bisphosphate and inositol 4-phosphate, and ultimately inositol. Lithium ions inhibited both the formation and dephosphorylation of inositol 4-monophosphate, which is a specific product of inositol polyphosphate metabolism. In addition, a cyclical metabolic sequence was initiated by the 3-phosphorylation of Ins-1,4,5-P3 to form inositol 1,3,4,5-tetrakisphosphate. The Ins-1,4,5-P3 3-kinase responsible for this reaction had a Km of 0.4 microM for Ins-1,4,5-P3 and a Vmax of 208 pmol/min/mg and was stimulated by increased Ca2+ concentrations in the micromolar range. Inositol 1,3,4,5-tetrakisphosphate was then dephosphorylated to inositol 1,3,4-trisphosphate, which in turn was either further degraded to inositol 3,4-bisphosphate or rephosphorylated to inositol 1,3,4,6-tetrakisphosphate. Lithium ions also inhibited the production of inositol 3,4-bisphosphate, explaining the large accumulation of inositol 1,3,4-trisphosphate in cells stimulated in the presence of lithium. Prolonged exposure to angiotensin II in the presence of Li+ caused a progressive decline in inositol polyphosphate formation without depletion of the lipid precursor, phosphatidyl-inositol 4,5-bisphosphate, suggesting that an accumulating product of polyphosphoinositide hydrolysis (possibly diacylglycerol) has an inhibitory effect on the phospholipase C-catalyzed breakdown process. These results indicate that, in addition to its breakdown by sequential dephosphorylations through Ins-1,4-P2 and Ins-4-P, Ins-1,4,5-P3 undergoes a complex series of phosphorylations and dephosphorylations to form at least two inositol tetrakisphosphates and their metabolites. These newly defined pathways may provide additional regulatory steps in the mechanism of cell activation by angiotensin II and other Ca2+-mobilizing hormones.
authors.affiliation: Endocrinology and Reproduction Research Branch, National Institute of Child Health and Human Development, Bethesda, Maryland 20892.
authors.forename: T
authors.initials: T
authors.lastname: Balla
authors.forename: A J
authors.initials: AJ
authors.lastname: Baukal
authors.forename: G
authors.initials: G
authors.lastname: Guillemette
authors.forename: K J
authors.initials: KJ
authors.lastname: Catt
mesh_terms: D000302:Adrenal Cortex / Q000187:drug effects* / Q000378:metabolism; D000804:Angiotensin II / Q000494:pharmacology*; D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D015544:Inositol 1,4,5-Trisphosphate; D007295:Inositol Phosphates / Q000378:metabolism*; D007536:Isomerism; D007700:Kinetics; D008094:Lithium / Q000494:pharmacology; D010539:Permeability; D011494:Protein Kinases / Q000378:metabolism; D013403:Sugar Phosphates / Q000378:metabolism*; D013997:Time Factors
chemical_list: D007295:Inositol Phosphates; D013403:Sugar Phosphates; D000804:Angiotensin II; D015544:Inositol 1,4,5-Trisphosphate; D008094:Lithium; D011494:Protein Kinases