Inhibition of protein non-enzymic glycation induced by Bendazac.
Enhanced non-enzymic glycation of proteins has been suggested to play a role in the pathogenesis of diabetic microangiopathy. Thus pharmacological inhibition of this reaction could be envisaged to delay the development of late diabetic complications. In the present study we have investigated the effect of a new compound, 1-Benzylindazole-3-oxyacetic acid, Bendazac (BDZ) on the in vitro glycation of soluble proteins (albumin and fibrinogen) and isolated glomerular basement membrane (GBM). The data obtained indicate that BDZ is capable of reducing significantly the glycation of albumin and fibrinogen (p less than 0.001). When present in concentrations usually found in patients undergoing therapy (40-80 micrograms/ml), an inhibitory effect on soluble proteins was also observed. Inhibition of glycation of GBM was found only in the presence of the active metabolite (5 hydroxy BDZ) and at high glucose concentrations. These results suggest that BDZ could interfere with protein non-enzymic glycation and its use in patients with diabetes may be then taken into consideration to evaluate the effect on late diabetic complications.
Bruno
M R
MR
Cattedra di Endocrinologia (I), Istituto II Clinica Medica, Policlinico Umberto I, Rome, Italy.
Sensi
M
M
Cioccia
G P
GP
Valente
L
L
Negri
M
M
Ghirlanda
G
G
Pozzilli
P
P
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D001485:Basement Membrane / Q000187:drug effects / Q000378:metabolism*; D002417:Cattle; D005340:Fibrinogen / Q000378:metabolism*; D006031:Glycosylation; D006801:Humans; D007191:Indazoles / Q000494:pharmacology*; D007678:Kidney Glomerulus / Q000187:drug effects / Q000378:metabolism*; D011720:Pyrazoles / Q000494:pharmacology*; D012709:Serum Albumin / Q000378:metabolism*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D007191:Indazoles; D011720:Pyrazoles; D012709:Serum Albumin; D005340:Fibrinogen; C000123:bendazac