Asialo von Willebrand factor enhances platelet adhesion to vessel subendothelium.
Native von Willebrand factor (N-vWF) binds to platelets activated by thrombin, ADP or ristocetin. Asialo vWF (As-vWF) induces platelet aggregation in absence of platelet activators. N-vWF mediates platelet adhesion to vessel subendothelium at high shear rates. We have investigated the role of As-vWF in supporting platelet deposition to rabbit vessel subendothelium at a shear rate of 2,000 sec-1, using the Baumgartner perfusion system. We have studied the effects of the addition of As-vWF (from 2 to 12 micrograms/ml) to perfusates consisting of washed red blood cells, 4% human albumin and washed platelets. Our results show a significant increase in platelet deposition on subendothelium (p less than 0.01) in perfusions to which As-vWF had been added. Blockage of the platelet glycoproteins Ib and IIb/IIIa (GPIb and GPIIb/IIIa) by specific monoclonal antibodies (LJIb1 and LJCP8, respectively) resulted in a decrease of platelet deposition in both types of perfusates prepared with N-vWF and As-vWF. Our results indicate that As-vWF enhances platelet deposition to vessel subendothelium under flow conditions. Furthermore, they suggest that this effect is mediated by the binding of As-vWF to platelet membrane receptors, which in turn, promote platelet spreading and adhesion to the subendothelium.
Bastida
E
E
Servicio Hemoterapia y Hemostasia, Facultad de Medicina, Universidad de Barcelona, Spain.
Monteagudo
J
J
Ordinas
A
A
De Marco
L
L
Castillo
R
R
D000911:Antibodies, Monoclonal; D001212:Asialoglycoproteins; D004730:Endothelium, Vascular / Q000502:physiology*; D010477:Perfusion; D010973:Platelet Adhesiveness; D012794:Sialic Acids / Q000302:isolation & purification; D014841:von Willebrand Factor / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000502:physiology
D000911:Antibodies, Monoclonal; D001212:Asialoglycoproteins; D012794:Sialic Acids; C044131:asialo-von Willebrand Factor; D014841:von Willebrand Factor