Polymorphism and complexity of HLA-DR: evidence for intra-HLA-DR region crossing-over events.
HLA-DR molecules were isolated from HLA-DR3, -5, and -w6 positive homozygous B-cell lines by immunoprecipitation with monoclonal antibodies and analyzed by gel electrophoretic techniques. DNA isolated from the same cell lines was digested with the restriction enzyme Taq I and hybridized with a DR beta full-length cDNA probe. We demonstrated that certain DR beta I alleles are found in combination with different DR beta III alleles as defined by Southern blotting, protein chemistry, a functional assay using purified protein derivative-specific T-cell lines, and, in one case, also alloreactive T-cell reagents. Our results indicate that within the family of HLA-DRw52-associated haplotypes DR beta chain genes may have been transferred from one haplotype to another. The implications of these findings are discussed.
Bontrop
R E
RE
Department of Immunohaematology and Blood Bank, University Hospital, Leiden, The Netherlands.
Tilanus
M G
MG
Mikulski
M M
MM
Elferink
D G
DG
Termijtelen
A
A
de Vries
R R
RR
van Rood
J J
JJ
Giphart
M J
MJ
D000483:Alleles; D000911:Antibodies, Monoclonal; D002460:Cell Line; D005796:Genes; D006681:HLA-D Antigens / Q000235:genetics*; D006684:HLA-DR Antigens / Q000032:analysis / Q000235:genetics*; D006801:Humans; D008213:Lymphocyte Activation; D008285:Major Histocompatibility Complex; D011110:Polymorphism, Genetic; D013601:T-Lymphocytes / Q000276:immunology
D000911:Antibodies, Monoclonal; D006681:HLA-D Antigens; D006684:HLA-DR Antigens