Infection of a murine T-cell line with a retrovirus carrying c-myc decreases the interleukin-2 cell dependence by a nonautocrine mechanism.
A murine interleukin-2 (IL-2)-dependent T-cell line, CTLL-2, was infected with a retrovirus carrying the mouse c-myc cDNA and the Tn-5 neogene. Transduced cells were selected in the presence of Geneticin sulfate (G418); these cells were shown to express both the endogenous and the transduced c-myc genes. The IL-2 requirement of these cells was then found to be significantly reduced. The cells did not express IL-2 mRNA nor did they produce an activity mitogenic for CTLL-2 cells. This suggests that the reduction of IL-2 dependence observed following retroviral transduction of c-myc is caused by a nonautocrine mechanism.
Shapiro
I M
IM
Department of Immunology, Karolinska Institute, Stockholm, Sweden.
Winberg
G
G
Stefanescu
I
I
Wigzell
H
H
Menezes
J
J
D000818:Animals; D002460:Cell Line; D007376:Interleukin-2 / Q000235:genetics / Q000494:pharmacology*; D007377:Interleukin-3 / Q000494:pharmacology; D051379:Mice; D011519:Proto-Oncogenes; D012333:RNA, Messenger / Q000032:analysis; D012190:Retroviridae / Q000235:genetics; D013601:T-Lymphocytes; D014161:Transduction, Genetic
D007376:Interleukin-2; D007377:Interleukin-3; D012333:RNA, Messenger