Immunohistochemical studies of cholecystokininlike peptides and their relation to 5-HT, CGRP, and bombesin immunoreactivities in the brainstem and spinal cord of lampreys.
The distribution of cholecystokinin (CCK)-like immunoreactivity in the brainstem and spinal cord of lampreys was studied by using CCK antisera with different properties. In the spinal cord, three separate systems reacted with CCK antisera: (1) A ventral and lateral fiber system descending from a group of neurons in the posterior reticular nucleus of the rhombencephalon was labeled by both a C-terminal-directed CCK antiserum and a monoclonal CCK antibody. (2) A dorsal root-dorsal column system of fibers originating from cell bodies in the dorsal root ganglia was labeled only by the C-terminal CCK antiserum. This CCK immunoreactivity could be abolished by preabsorption with calcitonin-gene-related peptide (CGRP), suggesting that it was due to cross-reactivity with a CGRP-like peptide. This system also contained 5-hydroxytryptamine (5-HT)-, bombesin-, and CGRP-like immunoreactivities. (3) An intraspinal system of 5-HT neurons was labeled with an antiserum to the midportion of CCK-33 but not by the other CCK antisera. The CCK labeling of this system was difficult to reduce by preabsorption with CCK peptide and thus appeared to be nonspecific. Groups of cell bodies in the middle reticular nucleus of the rhombencephalon, the reticular nucleus of the mesencephalon, and the hypothalamus were labeled by both the C-terminal and the monoclonal CCK antisera. The gut contained two types of CCK-like immunoreactivity, one of which appeared to be due to cross-reactivity with CGRP. A biochemical analysis showed that the content of CCK was low in the spinal cord compared to the brain, and these results agreed with the immunohistochemical findings.
Brodin
L
L
Nobel Institute for Neurophysiology, Stockholm, Sweden.
Buchanan
J T
JT
Hökfelt
T
T
Grillner
S
S
Rehfeld
J F
JF
Frey
P
P
Verhofstad
A A
AA
Dockray
G J
GJ
Walsh
J H
JH
D000818:Animals; D000911:Antibodies, Monoclonal; D001839:Bombesin / Q000032:analysis*; D001933:Brain Stem / Q000032:analysis*; D015740:Calcitonin Gene-Related Peptide; D002766:Cholecystokinin / Q000032:analysis*; D003429:Cross Reactions; D005399:Fishes / Q000378:metabolism*; D007150:Immunohistochemistry; D007798:Lampreys / Q000378:metabolism*; D009412:Nerve Fibers / Q000032:analysis; D009479:Neuropeptides / Q000032:analysis*; D012701:Serotonin / Q000032:analysis*; D013116:Spinal Cord / Q000032:analysis*; D014781:Viscera / Q000032:analysis / Q000294:innervation
D000911:Antibodies, Monoclonal; D009479:Neuropeptides; D012701:Serotonin; D002766:Cholecystokinin; D015740:Calcitonin Gene-Related Peptide; D001839:Bombesin