Production of a factor inhibiting tumor cell migration by spleen cells from tumor-bearing mice.
A factor inhibiting tumor cell migration was found in the culture supernatants of spleen cells of BALB/c mice transplanted with sarcoma cells 20 days before or injected with killed sarcoma cells 14 days before, when the spleen cells were stimulated in vitro for 2 or 24 hr with a 3 M KCl extract of the sarcoma. This factor did not inhibit the migration of guinea-pig macrophages or of theophylline-pretreated sarcoma cells. When the supernatants were fractioned on Sephadex G-100 columns, active fractions with molecular weights lower than 14,000 were distributed broadly.
Raichev
I
I
Department of Pathology, Pleven University School of Medicine, Bulgaria.
Dotchevska
R
R
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000276:immunology; D002464:Cell Migration Inhibition; D008222:Lymphokines / Q000096:biosynthesis*; D008264:Macrophages / Q000502:physiology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D012513:Sarcoma, Experimental / Q000276:immunology*; D013154:Spleen / Q000166:cytology / Q000378:metabolism*; D013806:Theophylline / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D008222:Lymphokines; C043874:tumor migration inhibition factor; D013806:Theophylline