Androgenic regulation of epidermal growth factor in the mouse ventral prostate.
Castration of adult male mice caused a marked reduction in the amount of immunoreactive epidermal growth factor (EGF) in the ventral prostate, and the treatment of such castrated mice with testosterone increased the EGF level significantly. Gel filtration of prostate extract showed that the immunoreactive EGF in the prostate had the same molecular weight (6,045) as the submandibular gland EGF. Moreover, its isoelectric point (pH 4.5) was almost similar to that (pH 4.55) of the submandibular gland peptide. These results suggest that under the control of androgens, mouse ventral prostate synthesizes EGF structurally and functionally identical to the submandibular gland EGF.
Hiramatsu
M
M
Department of Dental Pharmacology, School of Dentistry, Meikai University, Saitama, Japan.
Kashimata
M
M
Minami
N
N
Sato
A
A
Murayama
M
M
Minami
N
N
D000728:Androgens / Q000378:metabolism*; D000818:Animals; D004815:Epidermal Growth Factor / Q000378:metabolism*; D007526:Isoelectric Point; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008970:Molecular Weight; D009919:Orchiectomy; D011467:Prostate / Q000187:drug effects / Q000378:metabolism*; D013363:Submandibular Gland / Q000378:metabolism; D013739:Testosterone / Q000494:pharmacology
D000728:Androgens; D013739:Testosterone; D004815:Epidermal Growth Factor