Complete sequence-specific 1H nuclear magnetic resonance assignments for mouse epidermal growth factor.
The 1H NMR spectrum of the mouse epidermal growth factor (53 residues) was analyzed with the use of two-dimensional NMR techniques. All the observable 296 proton resonances were completely assigned in a sequential manner. For the spin system identification, two-dimensional homonuclear Hartmann-Hahn spectrum was useful, especially for arginine and proline residues. The easy spin system identification of these long-side-chain-bearing amino acid residues greatly facilitated the sequence-specific resonance assignment of the epidermal growth factor.
Kohda
D
D
Department of Molecular Physiology, Tokyo Metropolitan Institute of Medical Science.
Inagaki
F
F
D000596:Amino Acids / Q000032:analysis*; D000818:Animals; D004815:Epidermal Growth Factor / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy; D051379:Mice
D000596:Amino Acids; D004815:Epidermal Growth Factor