Production and isolation of reuterin, a growth inhibitor produced by Lactobacillus reuteri.
Lactobacillus reuteri is a prominent member of the Lactobacillus population in the gastrointestinal ecosystem of humans, poultry, swine, and other animals. Reuterin is a newly discovered, broad-spectrum antimicrobial substance produced by this species during fermentation of glycerol. In this report, we describe procedures for (i) producing reuterin in sufficient amounts to isolate from a fermentation mixture and (ii) isolating this substance by high-performance liquid chromatography. By using uniformly labeled [14C]glycerol, reuterin was identified as a product of glycerol fermentation associated with the production of beta-hydroxypropionic acid and trimethylene glycol.
Talarico
T L
TL
Department of Microbiology, North Carolina State University, Raleigh 27695.
Casas
I A
IA
Chung
T C
TC
Dobrogosz
W J
WJ
D000447:Aldehydes; D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000302:isolation & purification; D000935:Antifungal Agents / Q000302:isolation & purification*; D000981:Antiprotozoal Agents / Q000302:isolation & purification*; D005285:Fermentation; D005985:Glyceraldehyde / Q000031:analogs & derivatives; D005990:Glycerol / Q000378:metabolism; D006131:Growth Inhibitors / Q000096:biosynthesis* / Q000302:isolation & purification; D007778:Lactobacillus / Q000378:metabolism*; D011407:Propane
D000447:Aldehydes; D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D000981:Antiprotozoal Agents; D006131:Growth Inhibitors; C047158:3-hydroxypropionaldehyde; D005985:Glyceraldehyde; D005990:Glycerol; D011407:Propane