[Study of the age-related features of the structural state of rat liver cell chromatin using micrococcal nuclease and circular dichroism].
The structural state of liver cell chromatin of young (6 months-old) and aged rats was evaluated, using circular dichroism and DNA hydrolysis by micrococcal nuclease. It was found that the chromatin condensation ratio in active and non-active fractions increased upon ageing. The length of the DNA nucleosomal repeat did not change upon ageing in either chromatin fraction. An elevated chromatin condensation ratio was observed in aged rats both for the unfolded nucleosome chain and the supernucleosomal structures. The mechanisms of age-related increase in the chromatin compactization ratio at different levels of organization and in dissimilarly active fractions are different. It was suggested that the age-related differences in the degree of compactization may stimulate a decrease of the transcriptional activity of liver cell nuclei upon ageing.
Konoplia
E F
EF
Detinkin
O N
ON
Zhitkovich
A V
AV
D000375:Aging / Q000235:genetics*; D000818:Animals; D002843:Chromatin / Q000032:analysis*; D002942:Circular Dichroism; D004247:DNA / Q000378:metabolism; D006868:Hydrolysis; D008099:Liver / Q000032:analysis* / Q000166:cytology; D008836:Micrococcal Nuclease; D009707:Nucleosomes; D051381:Rats
D002843:Chromatin; D009707:Nucleosomes; D004247:DNA; D008836:Micrococcal Nuclease
Izuchenie vozrastnykh osobennosteĭ strukturnogo sostoianiia khromatina kletok pecheni krys s pomoshch'iu mikrokokkovoĭ nukleazy i krugovogo dikhroizma.