title: Basophils exhibit rearrangement of the bcr gene in Philadelphia chromosome-positive chronic myeloid leukemia.
abstract: Basophils were isolated from the peripheral blood of two patients with Philadelphia positive-chronic myeloid leukemia using monoclonal antibody Bsp-1 and fluorescence activated cell sorting. DNA blot analyses demonstrated rearrangement of the breakpoint cluster region gene in the isolated basophils, which suggests their leukemic origin. Isolated T cells from these patients that were cultured for 14 days in the presence of interleukin-2 lacked rearrangement of the breakpoint cluster region gene and are therefore unlikely to be derived from the chronic myeloid leukemia clone.
authors.affiliation: Cancer Society of New Zealand, Cytogenetics Unit, Christchurch Hospital.
authors.forename: T J
authors.initials: TJ
authors.lastname: Bosma
authors.forename: M A
authors.initials: MA
authors.lastname: Kennedy
authors.forename: M P
authors.initials: MP
authors.lastname: Bodger
authors.forename: P E
authors.initials: PE
authors.lastname: Hollings
authors.forename: P H
authors.initials: PH
authors.lastname: Fitzgerald
mesh_terms: D001491:Basophils / Q000032:analysis / Q000473:pathology*; D006801:Humans; D007951:Leukemia, Myeloid / Q000235:genetics* / Q000473:pathology; D009693:Nucleic Acid Hybridization; D009857:Oncogenes; D010677:Philadelphia Chromosome; D011995:Recombination, Genetic; D013601:T-Lymphocytes / Q000032:analysis / Q000473:pathology