Molecular forms of katacalcin, calcitonin gene-related peptide and gastrin-releasing peptide, in a human medullary thyroid carcinoma.
Peptides synthesized by a human medullary thyroid carcinoma were purified to homogeneity by reverse-phase high performance liquid chromatography and structurally characterized by determination of amino acid composition, amino acid sequence, and fast atom bombardment mass spectra. The katacalcin-related material in the tumor extract was heterogeneous. Katacalcin (1-21) represented the predominant molecular form but metabolites, identified as katacalcin (1-20), (1-19), (1-15) and (1-13), were also identified in high concentration. Calcitonin gene-related peptide-I was isolated from the tumor but calcitonin gene-related peptide-II was absent. A minor component of calcitonin gene-related peptide-like immunoreactivity was of higher molecular weight and may represent an incompletely processed form of the prohormone. Gastrin-releasing peptide (1-27) and gastrin-releasing peptide (18-27) (neuromedin C) were isolated from the tumor but gastrin-releasing peptide (14-27) and bombesin were absent.
Conlon
J M
JM
Clinical Research Group for Gastrointestinal Endocrinology Max-Planck-Gesellschaft, University of Göttingen, Federal Republic of Germany.
McGregor
G P
GP
Wallin
G
G
Grimelius
L
L
Thim
L
L
D000328:Adult; D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D002116:Calcitonin / Q000032:analysis* / Q000302:isolation & purification; D015740:Calcitonin Gene-Related Peptide; D002277:Carcinoma / Q000032:analysis*; D019886:Gastrin-Releasing Peptide; D006801:Humans; D008297:Male; D009479:Neuropeptides / Q000032:analysis* / Q000302:isolation & purification; D010446:Peptide Fragments / Q000032:analysis* / Q000302:isolation & purification; D010455:Peptides / Q000032:analysis* / Q000302:isolation & purification; D013964:Thyroid Neoplasms / Q000032:analysis*
D000596:Amino Acids; D009479:Neuropeptides; D010446:Peptide Fragments; D010455:Peptides; D019886:Gastrin-Releasing Peptide; C037553:katacalcin; D002116:Calcitonin; D015740:Calcitonin Gene-Related Peptide