[Production kinetics of T-lymphocyte activity regulating human granulopoiesis in vitro].
Human T-lymphocytes stimulated with Con A are able to produce both a granulocyte-colony-stimulating activity (G-CSA) and a colony-inhibiting activity. We have studied here the cumulative and daily non-cumulative production of these 2 activities. The release of G-CSA is low on the 1st day of culture, maximal production occurred between the 2nd and the 4th day; from the 6th day on, T-lymphocytes are unable to produce CSA. Colony-inhibiting activity production is usually weak at the beginning of culture, but constantly found on day 5 and on day 7. In conclusion, cumulative culture for 5 days seems optimal for the simultaneous detection of both activities.
Bonnefoix
T
T
Service d'Hématologie, CHUR, Grenoble, France.
Piccinni
M P
MP
Sotto
J J
JJ
D002453:Cell Cycle; D003115:Colony-Stimulating Factors / Q000378:metabolism / Q000494:pharmacology; D006098:Granulocytes / Q000502:physiology*; D006410:Hematopoiesis; D006801:Humans; D008213:Lymphocyte Activation; D013601:T-Lymphocytes / Q000378:metabolism / Q000502:physiology*; D013997:Time Factors
D003115:Colony-Stimulating Factors
Cinétique de production des activités lymphocytaires T régulant la granulopoïèse humaine in vitro.