title: Isolation of Salmonella typhi from standard whole-blood culture vs blood-clot cultures.
abstract: The use of 10% oxgall and bile broth medium, both supplemented with freshly prepared 100 u/ml streptokinase, for isolating Salmonella typhi by clot culture technique was evaluated and compared against whole blood culture systems (3 ml blood in 9 ml media and 8 ml blood in 72 ml media). These gave a 1:4 and 1:10 blood to medium ratio, respectively. Clot cultures in 10% oxgall (CLOX) gave a 57% positive isolation rate for S. typhi. A similar result was obtained from clot cultures in bile broth medium (CLBB). A total of 184 samples identified as positive for S. typhi were tested. There was no significant difference between the use of 10% oxgall or bile broth medium when used for clot culture. The whole blood culture systems still showed a significantly better rate of isolation than the clot culture methods.
authors.affiliation: U.S. Naval Medical Research Unit No. 2, Jakarta, Indonesia.
authors.forename: P
authors.initials: P
authors.lastname: Tjaniadi
authors.forename: E M
authors.initials: EM
authors.lastname: Lane
authors.forename: M
authors.initials: M
authors.lastname: Lesmana
authors.forename: D C
authors.initials: DC
authors.lastname: Edman
authors.forename: D
authors.initials: D
authors.lastname: Kostermans
mesh_terms: D001769:Blood / Q000382:microbiology*; D003470:Culture Media; D006801:Humans; D012485:Salmonella typhi / Q000302:isolation & purification*; D013300:Streptokinase
chemical_list: D003470:Culture Media; D013300:Streptokinase