Oxygen activation and ligand binding by pure heme-octapeptide microperoxidase-8 (MP-8).
The rapid, efficient preparation of pure microperoxidase-8 (MP-8) is described. Ligand binding studies confirm that MP-8 is monomeric in alkaline solution. It is shown that the monomeric MP-8 activates oxygen in a similar manner to that already reported for alkaline hemin, establishing the octapeptide as a possible second generation model for the oxygen activation/insertion reactions of the cytochrome P-450.
Adams
P A
PA
MRC Biomembrane Research Unit, University of Cape Town, Medical School, Republic of South Africa.
Byfield
M P
MP
Milton
R C
RC
Pratt
J M
JM
D000818:Animals; D006736:Horses; D008024:Ligands; D010100:Oxygen / Q000378:metabolism*; D010544:Peroxidases / Q000302:isolation & purification / Q000378:metabolism*; D013552:Swine
D008024:Ligands; D010544:Peroxidases; C021799:microperoxidase; D010100:Oxygen