Tissue levels of prostaglandins and what do they mean? Studies on the guinea-pig uterus.
The ratios of the concentrations of PGF2 alpha, PGE2 and 6-keto-PGF1 alpha in guinea-pig uterine horns, which were removed and placed in ethanol in 1.5 to 2 min, were 0.3:1.0:0.6 on day 7 and 13.8:1.0:0.8 on day 15 of the oestrous cycle. Adding indomethacin (10 micrograms/ml) to the ethanol had no significant effect on the tissue levels observed. These ratios were similar to the ratios of the outputs of PGF2 alpha, PGE2 and 6-keto-PGF1 alpha from the guinea-pig uterus (0.6:1.0:0.9 on day 7 and 7.6:1.0:1.5 on day 15), but were different (particularly on day 7, but only for 6-keto-PGF1 alpha on day 15) to the ratios of the amounts of the three PGs synthesized by homogenates of the guinea-pig uterus (7.2:1.0:2.4 on day 7 and 11.7:1.0:3.3 on day 15). Consequently, the measurement of tissue levels of PGs in the guinea-pig uterus reflects PG synthesis by intact tissue and changes in this synthesis, rather than PG synthesis by homogenates (broken cell preparations). Therefore, it appears meaningful to measure levels of PGs in the guinea-pig uterus since they reflect uterine PG output. Separation of the endometrium from the myometrium, which involved handling and mild trauma, stimulated uterine PG levels, but the ratio of the levels of PGF2 alpha, PGE2 and 6-keto-PGF1 alpha in the endometrium was still similar to that found in the non-separated uterus.
Poyser
N L
NL
Department of Pharmacology, University of Edinburgh, Scotland, U.K.
D015121:6-Ketoprostaglandin F1 alpha / Q000032:analysis; D000818:Animals; D015237:Dinoprost / Q000032:analysis; D015232:Dinoprostone / Q000032:analysis; D004717:Endometrium / Q000032:analysis; D005260:Female; D006168:Guinea Pigs; D007213:Indomethacin / Q000494:pharmacology; D009215:Myometrium / Q000032:analysis; D009928:Organ Specificity; D011453:Prostaglandins / Q000032:analysis*; D012016:Reference Values; D014599:Uterus / Q000032:analysis* / Q000187:drug effects
D011453:Prostaglandins; D015121:6-Ketoprostaglandin F1 alpha; D015237:Dinoprost; D015232:Dinoprostone; D007213:Indomethacin