Stimulation of murine hemopoietic colony formation by human IL-6.
A novel hemopoietic CSF has been identified in the medium conditioned by lectin-stimulated human T cells. The cDNA clone encoding this factor, isolated by functional expression cloning in monkey cos-1 cells, proved to be identical with the cDNA encoding the cytokine B cell stimulatory factor-2/IFN-beta 2, a factor now known as IL-6. In the murine system, IL-6 indirectly supports the formation of several different types of hemopoietic colonies, including those derived from early blast cells, and directly supports the proliferation of granulocyte/macrophage progenitors. These results expand the range of known target cells of IL-6 to include hemopoietic progenitors in addition to B cells, T cells, and fibroblasts and provide further evidence that this cytokine plays an important role within a network of interacting cytokines that regulates many different biologic responses.
Wong
G G
GG
Genetics Institute, Inc., Cambridge, MA 02140.
Witek-Giannotti
J S
JS
Temple
P A
PA
Kriz
R
R
Ferenz
C
C
Hewick
R M
RM
Clark
S C
SC
Ikebuchi
K
K
Ogawa
M
M
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D003115:Colony-Stimulating Factors / Q000494:pharmacology*; D005472:Fluorouracil / Q000494:pharmacology; D006133:Growth Substances / Q000494:pharmacology; D006410:Hematopoiesis / Q000187:drug effects*; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D015850:Interleukin-6; D007378:Interleukins / Q000494:pharmacology*; D051379:Mice; D008970:Molecular Weight; D013154:Spleen / Q000166:cytology
D003115:Colony-Stimulating Factors; D006133:Growth Substances; D015850:Interleukin-6; D007378:Interleukins; D005472:Fluorouracil