Simultaneous determination of ofloxacin, fenbufen and felbinac in rat plasma by high-performance liquid chromatography.
Ofloxacin, fenbufen and its active metabolite, felbinac, were simultaneously extracted from 50 microliters of rat plasma and analysed by high-performance liquid chromatography on a reversed-phase column. Quantitative and reproducible determinations were possible for ofloxacin, fenbufen and felbinac over the concentration ranges 0.15-40, 0.3-80 and 0.45-45 micrograms/ml, respectively. The detection limits for all the drugs were lower than those reported previously. The recovery of ofloxacin, fenbufen and felbinac added to plasma was nearly 100% with a coefficient of variation of less than 3.0%. This method was found to be applicable to pharmacokinetic studies of each drug after the concomitant administration of ofloxacin and fenbufen.
Katagiri
Y
Y
Department of Pharmacy, Shimane Medical University Hospital, Japan.
Naora
K
K
Ichikawa
N
N
Hayashibara
M
M
Iwamoto
K
K
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D008297:Male; D015242:Ofloxacin / Q000097:blood*; D010648:Phenylacetates / Q000097:blood*; D010654:Phenylbutyrates / Q000097:blood*; D051381:Rats; D011919:Rats, Inbred Strains
D000894:Anti-Inflammatory Agents, Non-Steroidal; D010648:Phenylacetates; D010654:Phenylbutyrates; C012776:biphenylylacetic acid; C010725:fenbufen; D015242:Ofloxacin