title: Cellular and serum immune response to dinitrophenol in adult Rana pipiens.
abstract: A rabbit anti-Rana anti-DNP antiserum was used in order to estimate changes in frequency of bone marrow (BM) cells containing cytoplasmic anti-DNP antibodies (cId+) for eight weeks following a single injection. We found that (i) cId+ cells increased from 0.2 to 4.3 percent of total mononuclear cells (MNC's) during the first 8 weeks, (ii) BM granulocytes increased in frequency up to week 2, then gradually decreased to week 8, and (iii) serum anti-DNP antibody levels, evaluated by ELISA, increased to week 8. Our observations (i) support those which suggest that Rana BM resembles mammaliam BM as a source of antibody-producing cells, (ii) indicate that specific immune responses in BM may result in a 20-fold increase in the frequency of antigen-related plasma cells among total MNC's, and (iii) suggest that BM B cell clonal expansion, maturation and secretion may result in an increase in specific antibody levels in serum.
authors.affiliation: Department of Biology, Carroll College, Waukesha, Wisconsin 53186.
authors.forename: L D
authors.initials: LD
authors.lastname: Zettergren
authors.forename: B W
authors.initials: BW
authors.lastname: Boldt
authors.forename: L S
authors.initials: LS
authors.lastname: Schmid
mesh_terms: D000818:Animals; D000917:Antibody Formation; D000921:Antibody-Producing Cells / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology; D001853:Bone Marrow / Q000276:immunology; D004140:Dinitrophenols / Q000276:immunology*; D006098:Granulocytes / Q000276:immunology; D007107:Immune System / Q000502:physiology*; D007111:Immunity, Cellular; D011894:Rana pipiens / Q000276:immunology*
chemical_list: D004140:Dinitrophenols