title: Protein kinase C is required for responses to T cell receptor ligands but not to interleukin-2 in T cells.
abstract: We have tested the role of protein kinase C in mRNA expression and T cell proliferation mediated through the T cell receptor and through the interleukin-2 (IL-2) receptor. Chronic treatment of a mouse T cell clone with phorbol esters caused a complete loss of protein kinase C activity and a concomitant loss of proliferation to T cell receptor ligands (antigen, lectins, antireceptor antibodies). In contrast, kinase C-depleted T cells retained the ability to proliferate to IL-2. Loss of the T cell receptor response was not due to decreased cell surface expression of receptor or impairment of early receptor function (phosphatidylinositol turnover, calcium mobilization). Kinase C-depleted T cells showed no induction of mRNAs for activation-associated genes on exposure to the T cell receptor ligand Concanavalin A; expression of a subset of the same mRNAs in response to IL-2 was unaffected. We conclude that kinase C is required for mRNA expression and subsequent proliferation mediated through the T cell receptor pathway but is not involved in mRNA expression and proliferation in response to IL-2.
authors.affiliation: Dana-Farber Cancer Institute, Harvard Medical School Boston, Massachusetts 02115.
authors.forename: V E
authors.initials: VE
authors.lastname: Valge
authors.forename: J G
authors.initials: JG
authors.lastname: Wong
authors.forename: B M
authors.initials: BM
authors.lastname: Datlof
authors.forename: A J
authors.initials: AJ
authors.lastname: Sinskey
authors.forename: A
authors.initials: A
authors.lastname: Rao
mesh_terms: D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002999:Clone Cells; D007376:Interleukin-2 / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D010703:Phorbol Esters / Q000494:pharmacology; D011493:Protein Kinase C / Q000378:metabolism*; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism*; D013601:T-Lymphocytes / Q000187:drug effects / Q000201:enzymology*
chemical_list: D007376:Interleukin-2; D010703:Phorbol Esters; D011948:Receptors, Antigen, T-Cell; D011493:Protein Kinase C