Determination of cytopathogenic effects of Trichomonas vaginalis on cultured cells by assaying 51Cr release.
By use of an assay for 51Cr release, it was attempted to determine the cytopathogenic effects of Trichomonas vaginalis on mammalian cultured cells. 51Cr was released from Na2(51)CrO4-labeled HeLa, L-929, FL, Vero and LLC-MK 2 cells. HeLa cells were most sensitive to the damage, followed by L-929, FL, Vero and LLC-MK 2 cells. The effect depended upon the number of parasite and the incubation period. The maximum 51Cr release from the labeled cells was observed at 37 degrees C and pH 6.0. These results attest that this assay is reliable for determining the cytopathogenic effects of T. vaginalis on the target cells.
Fukui
K
K
Department of Veterinary Science, College of Agriculture, University of Osaka Prefecture, Japan.
Takamori
Y
Y
D000818:Animals; D002460:Cell Line; D002860:Chromium Radioisotopes; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D013696:Temperature; D014246:Trichomonas vaginalis / Q000472:pathogenicity*; D014709:Vero Cells
D002860:Chromium Radioisotopes