[Simultaneous determination of vinburnine and 6-hydroxyvinburnine in human plasma by high-performance liquid chromatography].
An isocratic high-performance liquid chromatographic method has been developed to allow the simple and rapid determination of both vinburnine (I) and its main metabolite, 6-hydroxyvinburnine (II), in heparinized human plasma (0.5 ml). Compounds I and II and p-chlorodisopyramide (internal standard) were first extracted with alkalinized ethyl acetate and then with sulphuric acid. Separation was achieved on a reversed-phase muBondapak C18 column with a mobile phase of acetonitrile-water-0.1 M heptanesulphonate in acetic acid and with detection at 254 nm. Each run required 20 min. The within-day coefficients of variation for identical samples (20 ng/ml) were 7 and 6% and between-day coefficients of variation 8 and 26% for I and II, respectively. The detection limit was 5 ng/ml (normal therapeutic concentration, 10-300 ng/ml). The application of the method to drug monitoring was compared to that of a thin-layer chromatographic procedure.
Sado
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PA
Laboratoire de Pharmacie Galénique et Biopharmacie, Faculté de Médecine et Pharmacie, Rennes, France.
Gibassier
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D
Leverge
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R
D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D012015:Reference Standards; D014748:Vinca Alkaloids / Q000097:blood*
D014748:Vinca Alkaloids; C059426:6-hydroxyvinburnine; C016422:eburnamonine
Dosage simultané de la vinburnine et de la 6-hydroxyvinburnine par chromatographie liquide à haute performance.