title: Measurement of underivatized propranolol enantiomers in serum using a cellulose-tris(3,5-dimethylphenylcarbamate) high-performance liquid chromatographic (HPLC) chiral stationary phase.
abstract: A commercially available high-performance liquid chromatographic (HPLC) chiral stationary phase (HPLC-CSP) has been used to measure serum levels of d- and l-propranolol. The HPLC-CSP is based upon cellulose-tris(3,5-dimethylcarbamate) and is able to stereochemically resolve d- and l-propranolol without precolumn derivatization using a mobile phase composed of hexane:2-propranol:N,N-dimethyloctylamine (92:8:0.01, v/v/v). Under these conditions the observed stereochemical resolution (alpha) of the two enantiomers was alpha = 2.2. A subject's concentration-time curve of the two isomers was determined following the ingestion of 160 mg racemic propranolol.
authors.affiliation: Department of Clinical Pharmacy, University of Tennessee, Memphis 38163.
authors.forename: R J
authors.initials: RJ
authors.lastname: Straka
authors.forename: R L
authors.initials: RL
authors.lastname: Lalonde
authors.forename: I W
authors.initials: IW
authors.lastname: Wainer
mesh_terms: D002482:Cellulose; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D011433:Propranolol / Q000097:blood*; D012015:Reference Standards; D015203:Reproducibility of Results; D013237:Stereoisomerism; D014325:Tromethamine
chemical_list: D014325:Tromethamine; D002482:Cellulose; D011433:Propranolol