[The effect of Campath-1 on T- and NK-cells].
An assay system capable of detecting 0.03% residual T cells is described. This test system was used to evaluate bone marrow which was treated with Campath-1 and human complement (HC'). All T cell-depleted samples tested were found to be free of OKT3-positive T cells (less than 0.03%). The assay described provides a highly sensitive method for the detection of residual T cells and can be used as an alternative to limiting dilution assays. The results presented here confirm that treatment of donor bone marrow with Campath-1 and HC' provides a highly effective means of removing T cells and thus should be effective in GVHD prevention. However, although Campath-1 effectively depletes T- and B cells, it unexpectedly failed to eliminate cells that display NK function.
Schofer
O
O
Universitäts-Kinderklinik Mainz.
Veit
B C
BC
Johnson
F L
FL
D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D001402:B-Lymphocytes / Q000187:drug effects; D001853:Bone Marrow / Q000187:drug effects; D001854:Bone Marrow Cells; D002469:Cell Separation; D002478:Cells, Cultured; D003165:Complement System Proteins / Q000276:immunology*; D005434:Flow Cytometry; D006801:Humans; D007694:Killer Cells, Natural / Q000166:cytology* / Q000187:drug effects; D013601:T-Lymphocytes / Q000166:cytology* / Q000187:drug effects
D000911:Antibodies, Monoclonal; D003165:Complement System Proteins
Wirkung von Campath-1 auf T- und NK-Zellen.