Isolation, characterization, and molecular cloning of cryptic plasmids isolated from Edwardsiella ictaluri.
Fifty-five isolates of Edwardsiella ictaluri were examined for the presence of plasmid DNA by a rapid alkaline extraction procedure. All 49 isolates from channel catfish and a single isolate from Bengal danio carried 2 plasmids with molecular masses of approximately 3.2 and 3.7 megadaltons (Mdal). Five E ictaluri isolates from other fish contained 1 to 3 plasmids, which had molecular masses ranging from 2.5 to 45 Mdal. The 2 plasmids (3.2 and 3.7 Mdal) from the type strain of E ictaluri (ATCC 33202) were ligated into pUC19 cloning vectors, and restriction endonuclease maps of each insert were prepared.
Newton
J C
JC
Department of Pathobiology, College of Veterinary Medicine, Auburn University, AL 36849-3501.
Bird
R C
RC
Blevins
W T
WT
Wilt
G R
GR
Wolfe
L G
LG
D000818:Animals; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D004755:Enterobacteriaceae / Q000235:genetics* / Q000472:pathogenicity; D005399:Fishes; D007059:Ictaluridae; D010957:Plasmids; D015183:Restriction Mapping; D014774:Virulence
D004269:DNA, Bacterial