title: Anti HLA class I monoclonal antibody effect on PKC kinetics in PHA activated human peripheral blood mononuclear and E+ cells.
abstract: Cytoplasmic protein kinase C (PKC) has been studied in phytohemagglutinin (PHA) activated peripheral blood mononuclear cells (PBMC) and macrophage depleted E+ cell culture. Within 10' after contemporanous addition of PHA and anti HLA class I monoclonal antibody 01.65 (MoAb) PKC is depleted in both cell types. Enzyme activity recovers in the following hours however at 72 hours is at control values in E+ cultures while in PBMC cultures it is still depleted at 68% of the control. Anti HLA class I MoAb induced tritiated lymidine (3H-TdR) incorporation inhibition appears to be related to low levels of PKC activity.
authors.affiliation: Istituto di Genetica Medica, Universita' degli Studi di Ferrara, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Poltronieri
authors.forename: E
authors.initials: E
authors.lastname: Melloni
authors.forename: M
authors.initials: M
authors.lastname: Rubini
authors.forename: R
authors.initials: R
authors.lastname: Selvatici
authors.forename: C
authors.initials: C
authors.lastname: Mazzilli
authors.forename: R
authors.initials: R
authors.lastname: Baricordi
authors.forename: E
authors.initials: E
authors.lastname: Gandini
mesh_terms: D000911:Antibodies, Monoclonal; D002478:Cells, Cultured; D004261:DNA Replication; D015395:Histocompatibility Antigens Class I / Q000276:immunology*; D006801:Humans; D007700:Kinetics; D008213:Lymphocyte Activation; D011493:Protein Kinase C / Q000276:immunology / Q000378:metabolism*; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000166:cytology / Q000201:enzymology* / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D015395:Histocompatibility Antigens Class I; D011493:Protein Kinase C