title: Fast protein liquid chromatography of lymphocyte blastogenesis inhibitory factor produced by the human macrophage-like cell line U937.
abstract: The human macrophage-like cell line U937 constitutively produces a lymphocyte blastogenesis inhibitory factor (LBIF). Using fast protein liquid chromatography (FPLC)-chromatofocusing, FPLC-anion-exchange chromatography and high-performance liquid chromatography-gel permeation chromatography, LBIF has been purified to homogeneity. It is a single polypeptide chain and has a molecular weight of 57,000 daltons according to sodium dodecyl sulphate polyacrylamide gel electrophoresis under reducing conditions. LBIF shows pI 4.28-4.56 upon chromatofocusing on Mono P.
authors.affiliation: Institute of Immunological Science, Hokkaido University, Supporo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Sugimura
authors.forename: K
authors.initials: K
authors.lastname: Tsukahara
authors.forename: Y
authors.initials: Y
authors.lastname: Ueda
authors.forename: K
authors.initials: K
authors.lastname: Takeda
authors.forename: Y
authors.initials: Y
authors.lastname: Habu
authors.forename: I
authors.initials: I
authors.lastname: Azuma
mesh_terms: D000818:Animals; D002460:Cell Line; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D002853:Chromatography, Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D007375:Interleukin-1 / Q000037:antagonists & inhibitors; D007525:Isoelectric Focusing; D008222:Lymphokines / Q000302:isolation & purification*; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D013601:T-Lymphocytes / Q000187:drug effects
chemical_list: D007375:Interleukin-1; D008222:Lymphokines