Uridylation of U6 RNA in a nuclear extract in Ehrlich ascites tumor cells.
The uridylation of U6 RNA in a nuclear extract of Ehrlich ascites tumor cells was examined. This reaction required ATP or GTP, although these nucleotides were not incorporated into U6 RNA itself. ATP and GTP could be replaced by their nonhydrolyzable analogues ATP gamma S and GTP gamma S. Therefore, hydrolysis of ATP or GTP is not necessary for the uridylation of U6 RNA, indicating that these nucleotides are effectors of this reaction. By chromatographies of a nuclear extract of Ehrlich ascites tumor cells on phosphocellulose and DEAE-cellulose, U6 RNA could be separated from an enzyme adding a uridine residue(s) to this RNA.
Hirai
H
H
Faculty of Pharmaceutical Sciences, University of Tokyo.
Lee
D I
DI
Natori
S
S
Sekimizu
K
K
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000378:metabolism; D006868:Hydrolysis; D012334:RNA, Neoplasm / Q000378:metabolism*; D012342:RNA, Small Nuclear / Q000378:metabolism*; D014529:Uridine / Q000378:metabolism*; D014544:Uridine Triphosphate / Q000378:metabolism
D012334:RNA, Neoplasm; D012342:RNA, Small Nuclear; D006160:Guanosine Triphosphate; D000255:Adenosine Triphosphate; D014544:Uridine Triphosphate; D014529:Uridine