Molecular linkage of the Ly-3 and Ly-2 genes. Requirement of Ly-2 for Ly-3 surface expression.
We have isolated the Ly-3 gene by chromosomal walking from the previously cloned Ly-2 gene. These genes are shown to be 36 kb apart and in the same transcriptional orientation. Transfection of the Ly-3 gene into mouse L cells results in cell surface expression of Ly-3 protein only in the presence of Ly-2 (or its human homolog, CD8), although Ly-2 surface expression is not similarly dependent on Ly-3. cDNA clones encoding Ly-3 have been isolated and sequenced and show little sequence similarity to Ly-2, whereas both Ly-2 and Ly-3 are homologous to Ig variable regions. One cDNA clone encodes a form of Ly-3 lacking a transmembrane region. Although two alleles of Ly-3 have been previously defined serologically, Northern blot analyses of Ly-3 mRNA from a series of inbred mouse strains show unusual polymorphisms in the lengths of Ly-3 mRNA species defining at least three allelic variants of this gene.
Gorman
S D
SD
Department of Medicine, Stanford University Medical Center, CA 94305.
Sun
Y H
YH
Zamoyska
R
R
Parnes
J R
JR
D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte / Q000235:genetics; D000950:Antigens, Ly / Q000235:genetics* / Q000302:isolation & purification; D001483:Base Sequence; D002462:Cell Membrane / Q000378:metabolism*; D003001:Cloning, Molecular; D005805:Genes, MHC Class I; D008040:Genetic Linkage; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats
D000945:Antigens, Differentiation, T-Lymphocyte; D000950:Antigens, Ly; D012333:RNA, Messenger