Positive ion fast atom bombardment mass spectrometry of reductively pyridylaminated maltooligosaccharides and its application to alpha-amylase assay.
Positive ion fast atom bombardment mass spectra of maltooligosaccharides reductively aminated with 2-aminopyridine (Gn-AP) contain abundant [M + H]+ ions. Determination of G2-AP and G3-AP produced from G5-AP by the action of alpha-amylases, based on the abundance of their [M + H]+ ions relative to that of cellobiose reductively aminated with 2-amino-6-methylpyridine as the internal standard, allowed rapid and reproducible assay of these enzymes. It was advantageous for clinical investigation that the proportion of pancreatic and salivary alpha-amylase activities could be determined.
Honda
S
S
Faculty of Pharmaceutical Sciences, Kinki University, Higashi-Osaka, Japan.
Kakehi
K
K
Ohmura
T
T
Morita
M
M
D000586:Amination; D000631:Aminopyridines; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D009844:Oligosaccharides; D010179:Pancreas / Q000201:enzymology; D012463:Saliva / Q000201:enzymology; D000516:alpha-Amylases / Q000032:analysis*
D000631:Aminopyridines; D009844:Oligosaccharides; C021705:maltooligosaccharides; D000516:alpha-Amylases; C032439:alpha-aminopyridine