Microencapsulation [corrected] of tumor cells and assay for selecting anticancer drugs.
A microencapsulation of living tumor cells by an improved membrane and droplet forming technique was established in our laboratory. This semipermeable microencapsulating membrane was impermeable to serum albumins (M.W. 66,000 or 45,000) and human hemoglobin (M.W. 64,000), but permitted passage of low molecular weight substances (alpha-Lactalbumin, or Trypsinogen; M.W. 14,200 or 24,000). The in vivo results showed that microencapsulated tumor cell lines (KB, human oral epidermoid cell; P-388 lymphocytic leukemia; GBM 8401/TSGH, glioma) and human colorectal carcinoma cells grew and proliferated exponentially within twenty days. The in vivo growth exhibited better than that in vitro. Histological and morphological findings of these four different kinds of tumor cells are similar to those of original tumor cells. Treatment of the microencapsulated tumor cells (MTC) with cytotoxic drugs (adriamycin, 5-fluorouracil and cyclophosphamide) in vitro showed no significant difference in percent inhibition (p greater than 0.05) between the encapsulated and non-encapsulated cells. The in vivo data indicated that different anti-cancer drugs had different inhibition effects. The results showed that the MTC model was useful for screening an appropriate cytotoxic drug and could be applied to clinical medicine in the near future.
Chen
C F
CF
Department of Medical Research, Tri-service General Hospital, National Defense Medical Center, Taperi, Taiwan, Republic of China.
Hwang
J M
JM
Jao
S W
SW
Leu
F J
FJ
Chen
K Y
KY
D000818:Animals; D002470:Cell Survival; D003520:Cyclophosphamide / Q000627:therapeutic use; D004058:Diffusion; D004317:Doxorubicin / Q000627:therapeutic use; D004354:Drug Screening Assays, Antitumor / Q000379:methods*; D005472:Fluorouracil / Q000627:therapeutic use; D006801:Humans; D051379:Mice; D008809:Mice, Inbred C3H; D008819:Mice, Nude; D008875:Middle Aged; D010539:Permeability; D014407:Tumor Cells, Cultured / Q000187:drug effects
D004317:Doxorubicin; D003520:Cyclophosphamide; D005472:Fluorouracil