Serum from partially hepatectomized rats induces primary hepatocytes to enter S phase: a role for prostaglandins?
Serum obtained from partially hepatectomized rats 1, 3 or 24 h after operation was more effective in stimulating DNA synthesis in primary adult rat hepatocytes than serum from sham-operated rats; exposure to the serum for 2 h was sufficient to promote growth. Serum from the partially hepatectomized rats contained elevated levels of PGE2 and PGF2 alpha; it promoted hepatocytes to release prostaglandins into their culture medium. Growth-promoting effects of the serum and its capacity to elicit prostaglandin release into the culture medium were inhibited by 0.1 mM-indomethacin or 1 mM-aspirin. 0.1 mM-indomethacin also prevented DNA synthesis if the inhibitor were added 4 h after growth had been initiated by serum from partially hepatectomized rats, suggesting that prostaglandins continue to be important for the maintenance of hepatocyte growth for at least 6 h.
Little
P
P
Department of Biochemistry, University of Oxford, UK.
Skouteris
G G
GG
Ord
M G
MG
Stocken
L A
LA
D000818:Animals; D002478:Cells, Cultured; D015237:Dinoprost / Q000494:pharmacology / Q000502:physiology*; D015232:Dinoprostone / Q000494:pharmacology / Q000502:physiology*; D007399:Interphase; D008099:Liver / Q000166:cytology* / Q000187:drug effects; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D015237:Dinoprost; D015232:Dinoprostone