title: Application of a human T cell line derived from a Sézary syndrome patient for human interleukin 4 assay.
abstract: Interleukin 4 (IL-4) is a multi-functional biological regulator molecule. We developed a simple assay system for human IL-4, using a IL-2-dependent T cell line designated Sez 627 cell line. Sez 627 cells were found to respond only to human IL-4 and human IL-2, but not to other available lymphokines. In combination with mouse IL-2-dependent CTLL-2 cell line, which responds to both human and murine IL-2 but not to human IL-4, human IL-4 activity can be discriminated from human IL-2 activity.
authors.affiliation: Chest Disease Research Institute, Faculty of Medicine, Kyoto University, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Maeda
authors.forename: T
authors.initials: T
authors.lastname: Noma
authors.forename: K
authors.initials: K
authors.lastname: Hama
authors.forename: T
authors.initials: T
authors.lastname: Honjo
mesh_terms: D001681:Biological Assay / Q000379:methods*; D002460:Cell Line; D015368:Human T-lymphotropic virus 1; D006801:Humans; D007376:Interleukin-2 / Q000276:immunology; D015847:Interleukin-4; D007378:Interleukins / Q000032:analysis* / Q000494:pharmacology; D008213:Lymphocyte Activation; D012751:Sezary Syndrome / Q000276:immunology*; D013045:Species Specificity; D013601:T-Lymphocytes / Q000276:immunology* / Q000382:microbiology
chemical_list: D007376:Interleukin-2; D007378:Interleukins; D015847:Interleukin-4