title: Ultrastructural changes produced in Ehrlich ascites carcinoma cells by ultraviolet-visible radiation in the presence of melanins.
abstract: Irradiation of Ehrlich ascites carcinoma (EAC) cells in the presence of pheomelanin, i.e., red hair melanin (RHM), has been reported to produce extensive cell lysis. Irradiation in the presence of eumelanin, i.e., black hair melanin (BHM), or irradiation in the absence of either type of melanin did not produce this effect. We observed that RHM particles penetrated the cell membrane without apparent structural damage to the cell or the cell membrane. Irradiation of the cells in the absence of melanin did not produce any changes in the ultrastructure of the cells. Incubation of the cells in the dark in the presence of RHM produced only minor structural, mainly cytoplasmic changes. Irradiation of the cells in the presence of RHM produced extensive ultrastructural changes prior to complete cell lysis; these changes were more severe than the effects of incubation of the cells in the dark in the presence of RHM. When the cells incubated in the dark or irradiated in the presence of latex particles or either one of the eumelanins particles, viz. BHM or synthetic dopa melanin, these particles did not penetrate into the cells or produce any ultrastructural changes. These particles were in fact not even ingested by the cells.
authors.affiliation: Department of Anatomy, University of Toronto, Ontario, Canada.
authors.forename: P J
authors.initials: PJ
authors.lastname: Lea
authors.forename: A
authors.initials: A
authors.lastname: Pawlowski
authors.forename: S D
authors.initials: SD
authors.lastname: Persad
authors.forename: I A
authors.initials: IA
authors.lastname: Menon
authors.forename: H F
authors.initials: HF
authors.lastname: Haberman
mesh_terms: D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000648:ultrastructure*; D002462:Cell Membrane / Q000187:drug effects / Q000528:radiation effects / Q000648:ultrastructure; D002467:Cell Nucleus / Q000187:drug effects / Q000528:radiation effects / Q000648:ultrastructure; D003594:Cytoplasmic Granules / Q000187:drug effects / Q000528:radiation effects / Q000648:ultrastructure; D008543:Melanins / Q000494:pharmacology*; D051379:Mice; D008854:Microscopy, Electron; D014466:Ultraviolet Rays
chemical_list: D008543:Melanins