title: Polymorphism and complexity of HLA-DR: evidence for intra-HLA-DR region crossing-over events.
abstract: HLA-DR molecules were isolated from HLA-DR3, -5, and -w6 positive homozygous B-cell lines by immunoprecipitation with monoclonal antibodies and analyzed by gel electrophoretic techniques. DNA isolated from the same cell lines was digested with the restriction enzyme Taq I and hybridized with a DR beta full-length cDNA probe. We demonstrated that certain DR beta I alleles are found in combination with different DR beta III alleles as defined by Southern blotting, protein chemistry, a functional assay using purified protein derivative-specific T-cell lines, and, in one case, also alloreactive T-cell reagents. Our results indicate that within the family of HLA-DRw52-associated haplotypes DR beta chain genes may have been transferred from one haplotype to another. The implications of these findings are discussed.
authors.affiliation: Department of Immunohaematology and Blood Bank, University Hospital, Leiden, The Netherlands.
authors.forename: R E
authors.initials: RE
authors.lastname: Bontrop
authors.forename: M G
authors.initials: MG
authors.lastname: Tilanus
authors.forename: M M
authors.initials: MM
authors.lastname: Mikulski
authors.forename: D G
authors.initials: DG
authors.lastname: Elferink
authors.forename: A
authors.initials: A
authors.lastname: Termijtelen
authors.forename: R R
authors.initials: RR
authors.lastname: de Vries
authors.forename: J J
authors.initials: JJ
authors.lastname: van Rood
authors.forename: M J
authors.initials: MJ
authors.lastname: Giphart
mesh_terms: D000483:Alleles; D000911:Antibodies, Monoclonal; D002460:Cell Line; D005796:Genes; D006681:HLA-D Antigens / Q000235:genetics*; D006684:HLA-DR Antigens / Q000032:analysis / Q000235:genetics*; D006801:Humans; D008213:Lymphocyte Activation; D008285:Major Histocompatibility Complex; D011110:Polymorphism, Genetic; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D006681:HLA-D Antigens; D006684:HLA-DR Antigens