[Establishment of a mammary cancer cell line Ca 761-86 and its biologic characteristics in inbred 615 mice].
A cell line designated as Ca761-86 has been established from the solid mouse mammary cancer (Ca761) by suspension culture. It has been passaged for more than 200 generations. Moderate round cells were predominant and most of them were mononuclear. Some characteristics of malignant cells and A-type viral-like particles were observed by electron microscopy. The results of cytochemical studies (DNA, RNA, SDH, 5'AMPase, ACP etc) were comparable to the ultramicroscopic results. It multiplied approximately 27.4 fold on Day 5 with mitotic index reaching 1.8% on Day 3. This cell line was a hyperdiploid with karyotype of 45 or 43, -2X, tri12, tri17, +M1-5. Cell agglutination was observed when treated with ConA (greater than or equal to 7 micrograms/ml). Spontaneous agglutination might also take place without adding any ConA. After 5 x 10(6) cells of Ca761-86 suspension were transplanted into the normal inbred 615 mice by different ways (subcutaneous, intrafoot pad or intraperitoneal), the transplantability rate reached 100%. Spontaneous remission was never observed and its metastatic ability reserved. PPLO were not detected. Ca761-86 may be of value for practical purposes.
Cheng
Y D
YD
Dalian Medical College.
D000818:Animals; D002460:Cell Line; D004171:Diploidy; D007621:Karyotyping; D008325:Mammary Neoplasms, Experimental / Q000473:pathology*; D051379:Mice; D008815:Mice, Inbred Strains; D009368:Neoplasm Transplantation; D014407:Tumor Cells, Cultured