title: Pancreastatin distribution and plasma levels in the pig.
abstract: Pancreastatin is a peptide isolated from porcine pancreas which has insulin-suppressive actions in vitro and sequence homology with chromogranin A. Using radioimmunoassay and immunocytochemistry we investigated whether pancreastatin has a more widespread distribution and a possible endocrine role in the pig. Pancreastatin immunoreactivity was found in plasma, adrenal gland, pancreas, anterior pituitary and throughout the gastrointestinal tract. The immunoreactivity was colocalized with chromogranin immunoreactivity in endocrine cells and ultrastructurally (in the pancreas) to storage granules. Characterization of pancreastatin-like immunoreactivity, using gel permeation and high performance liquid chromatography, separated 3 different pancreastatin-like immunoreactive forms: one molecular form, indistinguishable from synthetic pancreastatin 1-49, was predominant in pancreas and thyroid and released into the circulation postprandially. However, a high dose (greater than 1 nmol/l) infusion of pancreastatin 33-49 (the biologically active moiety in vitro) into conscious pigs had no effect on either basal or glucose-stimulated insulin secretion.
authors.affiliation: Department of Medicine, Royal Postgraduate Medical School Hammersmith Hospital, London, UK.
authors.forename: D
authors.initials: D
authors.lastname: Bretherton-Watt
authors.forename: M A
authors.initials: MA
authors.lastname: Ghatei
authors.forename: A E
authors.initials: AE
authors.lastname: Bishop
authors.forename: P
authors.initials: P
authors.lastname: Facer
authors.forename: M
authors.initials: M
authors.lastname: Fahey
authors.forename: M
authors.initials: M
authors.lastname: Hedges
authors.forename: G
authors.initials: G
authors.lastname: Williams
authors.forename: K L
authors.initials: KL
authors.lastname: Valentino
authors.forename: K
authors.initials: K
authors.lastname: Tatemoto
authors.forename: K
authors.initials: K
authors.lastname: Roth
mesh_terms: D000311:Adrenal Glands / Q000032:analysis; D000818:Animals; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D053379:Chromogranin A; D004386:Duodenum / Q000032:analysis; D005260:Female; D007150:Immunohistochemistry; D008297:Male; D009928:Organ Specificity; D010179:Pancreas / Q000032:analysis; D010187:Pancreatic Hormones / Q000032:analysis* / Q000097:blood; D011863:Radioimmunoassay; D013552:Swine / Q000378:metabolism*; D013961:Thyroid Gland / Q000032:analysis
chemical_list: D053379:Chromogranin A; D010187:Pancreatic Hormones; C050836:pancreastatin