Cell-mediated immune response toward viral envelope and core antigens in gibbon apes (Hylobates lar) chronically infected with human immunodeficiency virus-1.
The specific cellular immune response toward envelope and core proteins of human immunodeficiency virus-1 (HIV-1) was investigated in gibbon apes chronically infected with the HTLV-IIIB isolate. After in vitro stimulation of PBMC from infected and control animals with HIV-1 Ag, DNA synthesis, IL-2R expression and IL-2 release were assayed. Cells from infected gibbon apes demonstrated a group-specific response toward whole virus preparations from three divergent HIV-1 isolates (HTLV-IIIB, HTLV-IIIRF, HTLV-IIIMN). Consistent responses were also detected against purified HIV-1 Ag, i.e., native gp120 envelope glycoprotein, recombinant gp160 glycoprotein, a synthetic peptide (peptide 7) representing a highly conserved region of gp120, and purified native core protein p24. In addition, lymphocytes from infected gibbon apes displayed a specific, MHC-restricted, cytotoxic activity against autologous cells expressing HIV-1 envelope or gag proteins. The specific T cell reactivity toward HIV-1 proteins observed in infected gibbons contrasts with findings in HIV-1 infected humans, and may help to explain the apparent discrepancy in the natural history of the infection between the two species.
Lusso
P
P
Laboratory of Tumor Cell Biology, National Cancer Institute, Bethesda, MD 20892.
Markham
P D
PD
Ranki
A
A
Earl
P
P
Moss
B
B
Dorner
F
F
Gallo
R C
RC
Krohn
K J
KJ
D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology* / Q000378:metabolism; D000818:Animals; D002908:Chronic Disease; D003601:Cytotoxicity Tests, Immunologic; D015488:HIV Antigens / Q000276:immunology* / Q000302:isolation & purification; D006801:Humans; D006922:Hylobates; D007376:Interleukin-2 / Q000378:metabolism; D008213:Lymphocyte Activation; D015375:Receptors, Interleukin-2 / Q000032:analysis; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014758:Viral Core Proteins / Q000276:immunology* / Q000302:isolation & purification; D014759:Viral Envelope Proteins / Q000276:immunology* / Q000302:isolation & purification
D015488:HIV Antigens; D007376:Interleukin-2; D015375:Receptors, Interleukin-2; D014758:Viral Core Proteins; D014759:Viral Envelope Proteins