Application of high-performance liquid chromatography to the analysis of propionyl-L-carnitine by a stereospecific enzyme assay.
Propionyl-L-carnitine was converted by chemical hydrolysis (0.3 M potassium hydroxide, pH 12.8, room temperature) into L-carnitine, which competes with crotonoylbetaine formation and can be quantitatively evaluated by an enzyme assay. Under the conditions selected, hydrolysis of propionyl-L-carnitine to L-carnitine was completed in a few minutes with less than 0.5% of crotonoylbetaine being formed. The method described is enantioselective and possesses the analytical requirements for assaying propionyl-L-carnitine during chemical synthesis procedures and in pharmaceutical formulations.
Marzo
A
A
Real srl, Como, Italy.
Monti
N
N
Ripamonti
M
M
Arrigoni Martelli
E
E
D000818:Animals; D002331:Carnitine / Q000031:analogs & derivatives* / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D004285:Dogs; D006801:Humans; D006868:Hydrolysis; D051381:Rats; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism
C003223:propionylcarnitine; D002331:Carnitine