Enkephalin activity on antigen-induced proliferation of human peripheral blood mononucleate cells.
The effect of the two opioid pentapeptides met- and leu-enkephalin and of the tetrapeptide TYR-GLY-GLY-PHE on the stimulated proliferation of human peripheral blood mononucleate cells (PBMC) is described. Cell populations obtained from different donors were induced to proliferate with a Candida antigen. In the presence of the antigen, met-enkephalin and TYR-GLY-GLY-PHE had a concentration-dependent diphasic effect. The proliferation of PBMC was stimulated at high peptide concentration, while it was inhibited at the lower concentrations used. The effect of leu-enkephalin appears to be rather ambiguous, and directed towards the inhibition rather than the stimulation of PBMC proliferation.
Roscetti
G
G
Dipartimento di Medicina Sperimentale e Scienze Biochimiche, II Universita degli Studi di Roma, Tor Vergata, Rome, Italy.
Ausiello
C M
CM
Palma
C
C
Gulla
P
P
Roda
L G
LG
D004743:Enkephalin, Leucine / Q000494:pharmacology; D004744:Enkephalin, Methionine / Q000494:pharmacology; D004745:Enkephalins / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000276:immunology; D008213:Lymphocyte Activation / Q000187:drug effects*; D009842:Oligopeptides / Q000494:pharmacology
D004745:Enkephalins; D009842:Oligopeptides; D004744:Enkephalin, Methionine; D004743:Enkephalin, Leucine; C032272:tyrosyl-glycyl-glycyl-phenylalanyl