Oxidative damage to DNA and deoxyribose by beta-lactam antibiotics in the presence of iron and copper salts.
beta-lactam antibiotics in the presence of certain metal ions damage deoxyribose and DNA with the release of thiobarbituric acid-reactive material. This damage can be substantially prevented by catalase, metal chelators and some scavengers of the hydroxyl radical. Ferric salts in the presence of certain beta-lactam antibiotics were effective in degrading deoxyribose but they did not appear to damage DNA. In contrast copper salts and beta-lactam antibiotics were extremely effective in damaging both DNA and deoxyribose.
Quinlan
G J
GJ
Division of Chemistry, National Institute for Biological Standards and Control, South Mimms, Potters Bar, Herts.
Gutteridge
J M
JM
D000900:Anti-Bacterial Agents; D002374:Catalase; D003300:Copper; D004247:DNA; D004249:DNA Damage; D003855:Deoxyribose; D007202:Indicators and Reagents; D007501:Iron; D010084:Oxidation-Reduction; D013482:Superoxide Dismutase; D013858:Thiobarbiturates; D047090:beta-Lactams
D000900:Anti-Bacterial Agents; D007202:Indicators and Reagents; D013858:Thiobarbiturates; D047090:beta-Lactams; D003855:Deoxyribose; D003300:Copper; D004247:DNA; D007501:Iron; D002374:Catalase; D013482:Superoxide Dismutase; C029684:thiobarbituric acid