Anti HLA class I monoclonal antibody effect on PKC kinetics in PHA activated human peripheral blood mononuclear and E+ cells.
Cytoplasmic protein kinase C (PKC) has been studied in phytohemagglutinin (PHA) activated peripheral blood mononuclear cells (PBMC) and macrophage depleted E+ cell culture. Within 10' after contemporanous addition of PHA and anti HLA class I monoclonal antibody 01.65 (MoAb) PKC is depleted in both cell types. Enzyme activity recovers in the following hours however at 72 hours is at control values in E+ cultures while in PBMC cultures it is still depleted at 68% of the control. Anti HLA class I MoAb induced tritiated lymidine (3H-TdR) incorporation inhibition appears to be related to low levels of PKC activity.
Poltronieri
L
L
Istituto di Genetica Medica, Universita' degli Studi di Ferrara, Italy.
Melloni
E
E
Rubini
M
M
Selvatici
R
R
Mazzilli
C
C
Baricordi
R
R
Gandini
E
E
D000911:Antibodies, Monoclonal; D002478:Cells, Cultured; D004261:DNA Replication; D015395:Histocompatibility Antigens Class I / Q000276:immunology*; D006801:Humans; D007700:Kinetics; D008213:Lymphocyte Activation; D011493:Protein Kinase C / Q000276:immunology / Q000378:metabolism*; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000166:cytology / Q000201:enzymology* / Q000276:immunology
D000911:Antibodies, Monoclonal; D015395:Histocompatibility Antigens Class I; D011493:Protein Kinase C