A Dactylium dendroides morphological mutant defective in D-galactose metabolism.
1. A morphological mutant of the mold Dactylium dendroides was isolated and the phenotype characterized as D-Gal- and L-Ara-. 2. The transport system for D-galactose seemed to be inducible in wild type and mutant and was altered in the mutant. 3. Galactose-1-P-uridylyl transferase activity was absent in the mutant. 4. The levels of intracellular galactose oxidase activity were similar in the wild type and in the mutant, thereby excluding a possible participation of this enzyme in galactose catabolism in the mold. 5. The low level of galactose oxidase activity found in the extracellular medium indicates a defect in galactose oxidase secretion by the mutant.
Kubicki
D
D
Departamento de Bioquímica, Universidade Federal do Paraná, Curitiba, Brasil.
Zancan
G T
GT
Amaral
D
D
D001089:Arabinose / Q000378:metabolism; D005690:Galactose / Q000378:metabolism*; D005692:Galactose Oxidase / Q000172:deficiency / Q000378:metabolism*; D003904:Mitosporic Fungi / Q000201:enzymology / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D009154:Mutation
D001089:Arabinose; D005692:Galactose Oxidase; D005690:Galactose