Nucleotide sequence and expression of a Streptomyces griseosporeus proteinaceous alpha-amylase inhibitor (HaimII) gene.
The coding region of the alpha-amylase inhibitor (HaimII) gene from the producing strain Streptomyces griseosporeus YM-25 was localized on an 800-base-pair DNA segment. The nucleotide sequence of a 1,191-base-pair region including the HaimII gene was determined by the dideoxy-chain termination method. The nucleotide sequence data predicted an open reading frame of 363 base pairs starting with an ATG initiation codon and ending with a TGA translational stop codon. The amino acid sequence deduced from the nucleotide sequence indicated that the presumptive pre-HaimII protein extends 37 amino acids to the amino terminus and 6 amino acids to the carboxyl terminus of the mature HaimII protein. The pre-HaimII protein is believed to be processed both during and after secretion. Two forms of the inhibitor, which have a higher molecular weight than that of the HaimII protein isolated from S. griseosporeus, were partially purified from the culture filtrate of Streptomyces lividans containing the cloned HaimII gene.
Nagaso
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Institute of Applied Microbiology, University of Tokyo, Japan.
Saito
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Saito
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Takahashi
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D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D003062:Codon; D004269:DNA, Bacterial / Q000235:genetics; D004791:Enzyme Inhibitors / Q000235:genetics*; D005786:Gene Expression Regulation; D005798:Genes, Bacterial; D008970:Molecular Weight; D021382:Protein Sorting Signals; D013302:Streptomyces / Q000201:enzymology / Q000235:genetics*; D000516:alpha-Amylases / Q000037:antagonists & inhibitors*
D003062:Codon; D004269:DNA, Bacterial; D004791:Enzyme Inhibitors; D021382:Protein Sorting Signals; D000516:alpha-Amylases