A simple, inexpensive method for the salt-free concentration of proteins for analysis by two-dimensional gel electrophoresis.
A rapid, inexpensive method for the salt-free concentration of small quantities of proteins for analysis by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) has been developed. Proteins adsorbed to diatomaceous earth are subsequently removed using either sodium dodecyl sulfate or urea solubilization reagents for 2D-PAGE analysis. This procedure has been found to concentrate proteins having wide ranges of molecular weight and charge. It is also valuable for the concentration of large numbers of small samples from cells cultured in vitro.
Maresh
G A
GA
Department of Cell Biology, Baylor College of Medicine, Houston, TX 77030.
Dunbar
B S
BS
D000818:Animals; D002417:Cattle; D007692:Diatomaceous Earth; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D011506:Proteins / Q000302:isolation & purification*; D011817:Rabbits
D011506:Proteins; D007692:Diatomaceous Earth