title: A comparative 1H NMR study of mouse alpha(1-53) and beta(2-53) epidermal growth factors.
abstract: The three-dimensional structure of the mouse epidermal growth factor (EGF) in solution was studied by comparison of the 1H NMR spectra of alpha EGF (1-53) and beta EGF (2-53, des-asparaginyl 1 form). Using pH dependence of chemical shifts and a two-dimensional difference spectrum, the effect of the N-terminal deletion was investigated based on the complete assignment of the proton resonances. The affected residues were all found to be located exactly in the triple-stranded, beta-sheet core in the N-terminal domain of the EGF molecule.
authors.affiliation: Department of Molecular Physiology, Tokyo Metropolitan Institute of Medical Science, Japan.
authors.forename: D
authors.initials: D
authors.lastname: Kohda
authors.forename: C
authors.initials: C
authors.lastname: Kodama
authors.forename: R
authors.initials: R
authors.lastname: Kase
authors.forename: H
authors.initials: H
authors.lastname: Nomoto
authors.forename: K
authors.initials: K
authors.lastname: Hayashi
authors.forename: F
authors.initials: F
authors.lastname: Inagaki
mesh_terms: D000596:Amino Acids; D000818:Animals; D004815:Epidermal Growth Factor / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D051379:Mice; D011487:Protein Conformation; D013363:Submandibular Gland / Q000032:analysis
chemical_list: D000596:Amino Acids; D004815:Epidermal Growth Factor