Localization of the active principles of the male fern (Dryopteris filix-mas (L.) Schott.) by fluorescence microscopy.
The active principles of the male fern, Dryopteris filix-mas (L.) Schott., consisting of a mixture of phloroglucinol derivatives called "crude filicin", are localized by means of fluorescence microscopy under U.V. 365 nm and monochromatic blue 435-490 nm lights. Crude filicin is autofluorescent, and therefore may be easily localized in the internal glandular hairs of the intercellular spaces of rhizome and leaf bases parenchyma, without pre-treatment of sections. Localization is confirmed both morphologically and chemically, and is possible on a fresh material, as well as on a desiccated one. The test allows also a complete anatomical examination of sections, and may be useful, for its easiness and quickness, in further taxonomic or pharmacognostic studies.
Franchi
G G
GG
Dipartimento Farmaco Chimico Tecnologico, Università di Siena, Italy.
Ferri
S
S
D002090:Butyrophenones / Q000032:analysis*; D008856:Microscopy, Fluorescence; D010946:Plants, Medicinal / Q000032:analysis* / Q000033:anatomy & histology
D002090:Butyrophenones; C059517:filicin