Application of tris(2,2'-bipyridyl)ruthenium(III) for the investigation of DNA spatial structure by a chemical modification method.
It was shown that the treatment of a DNA fragment and a hepta-decanucleotide having a hairpin structure by Ru(2,2'-bipyridyl)3(3+) complex at 15 degrees C in 1 M NaCl for 10-60 min, and subsequent hydrolysis in 1 M piperidine at 95 degrees C led to the specific cleavage of the polynucleotide chain at guanosine residues in the single-stranded regions. This method can be used for the studying of nucleic acids secondary structure.
Fedorova
O S
OS
Institute of Bioorganic Chemistry, Siberian Division of the USSR Academy of Sciences, Novosibirsk.
Podust
L M
LM
D015082:2,2'-Dipyridyl / Q000031:analogs & derivatives; D004247:DNA / Q000032:analysis*; D009690:Nucleic Acid Conformation; D009942:Organometallic Compounds; D011725:Pyridines; D012428:Ruthenium
D009942:Organometallic Compounds; D011725:Pyridines; C058901:tris(2,2'-bipyridine)ruthenium III; D015082:2,2'-Dipyridyl; D012428:Ruthenium; D004247:DNA