A novel effect of EGF on mRNA stability.
Expression of the epidermal growth factor (EGF) receptor gene is stimulated by EGF and the phorbol ester, 4 beta-phorbol 12-myristate 13-acetate (PMA). PMA elevates EGF receptor mRNA levels in human KB epidermoid carcinoma cells, but does not significantly affect the half-life of this mRNA when its decay is examined after the addition of actinomycin D. In contrast, EGF greatly prolongs the half-life of EGF receptor mRNA suggesting a possible mechanism for the stimulatory effect of EGF on EGF receptor mRNA levels. EGF also stabilizes beta-tubulin and beta-actin mRNAs but has very little effect on the degradation of total mRNA.
Jinno
Y
Y
Laboratory of Molecular Biology, National Cancer Institute, Bethesda, MD 20892.
Merlino
G T
GT
Pastan
I
I
D000199:Actins / Q000235:genetics; D002294:Carcinoma, Squamous Cell / Q000473:pathology; D003609:Dactinomycin / Q000494:pharmacology; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D066246:ErbB Receptors / Q000235:genetics*; D006207:Half-Life; D006801:Humans; D012323:RNA Processing, Post-Transcriptional / Q000187:drug effects; D012333:RNA, Messenger / Q000378:metabolism*; D014404:Tubulin / Q000235:genetics; D014407:Tumor Cells, Cultured
D000199:Actins; D012333:RNA, Messenger; D014404:Tubulin; D003609:Dactinomycin; D004815:Epidermal Growth Factor; D066246:ErbB Receptors