3-H-ketanserin (serotonin type 2) binding in the rat frontal cortex: effect of immobilization stress.
The number of 3H-ketanserin (5-HT2) binding sites in rat frontal cortex was estimated in groups of male rats subjected to a single or repeated immobilization stress (IMO). After an acute IMO for 30 or 120 min the number of 5-HT2 binding sites was significantly increased. Similar increase was found even in a group subjected for 120 min IMO for 6 consecutive days and then allowed 24 h rest (i.e. repeatedly stressed controls). If similarly treated animals were subjected to 30 min IMO after 24 h rest, the number of binding sites was increased compared to repeatedly stressed controls, but this was not the case after 120 min IMO. No significant changes of 5-HT2 receptor affinity to 3H-ketanserin were found. The changes of the ratio of 5-hydroxyindolacetic acid/5-hydroxytryptamine were similar to those of binding sites. It was concluded that the enhanced number of cortical 5-HT2 receptor binding sites may result from accelerated serotonin turnover induced by stress, but the results did not indicate any direct association between the serotonin turnover and 5-HT receptor function in the rat frontal cortex.
Torda
T
T
Institute of Experimental Endocrinology, Slovak Academy of Sciences, Bratislava, Czechoslovakia.
Culman
J
J
Cechová
E
E
Murgas
K
K
D000818:Animals; D005625:Frontal Lobe / Q000378:metabolism*; D007103:Immobilization; D007650:Ketanserin / Q000378:metabolism*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D011985:Receptors, Serotonin / Q000378:metabolism*; D012701:Serotonin / Q000378:metabolism; D013312:Stress, Physiological / Q000378:metabolism*
D011985:Receptors, Serotonin; D012701:Serotonin; D007650:Ketanserin