title: Direct regulation of bone resorption of chick embryonic femur in organ culture by the change of calcium or phosphate ion concentration in the medium.
abstract: Bone resorbing activity was determined in organ culture by following the change of biological half life of 45Ca (T1/2) incorporated into bone salts of chick embryonic femur. The elevation of calcium or phosphate ion in the medium brought about an increase of T1/2, indicating that the bone resorption was inhibited. Conversely, the decrease of calcium or phosphate content in the medium resulted in the decrease of T1/2. A change of the magnesium ion concentration in the medium had no discernible effect on T1/2. These results suggest that a direct regulating system exists between bone resorption of chick embryonic femur and calcium or phosphate content in the medium and this phenomenon is specific for calcium or phosphate ion.
authors.affiliation: Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, Teikyo University, Kanagawa, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Kawashima
authors.forename: C
authors.initials: C
authors.lastname: Owa
authors.forename: H
authors.initials: H
authors.lastname: Endo
mesh_terms: D000818:Animals; D001862:Bone Resorption / Q000187:drug effects*; D002118:Calcium / Q000494:pharmacology*; D002642:Chick Embryo; D003470:Culture Media; D005269:Femur / Q000187:drug effects / Q000196:embryology; D006207:Half-Life; D008274:Magnesium / Q000494:pharmacology; D009924:Organ Culture Techniques; D010710:Phosphates / Q000494:pharmacology*
chemical_list: D003470:Culture Media; D010710:Phosphates; D008274:Magnesium; D002118:Calcium