O-acetylated sialic acids in gangliosides from pig spleen lymphocytes.
The sialic acid content of gangliosides from pig spleen lymphocytes was studied by thin-layer chromatography. N-glycolylneuraminic acid and N-acetylneuraminic acid were detected for the first time in this material as the major sialic acids. In addition, two other sialic acids, tentatively designated O-acetylated sialic acids, according to their RF values on cellulose plates, were also found. We have detected several gangliosides showing a retarded migration pattern in two dimensional thin-layer chromatography with an intermediate ammonia treatment. One of these gangliosides could be an O-acetylated derivative of the disialoganglioside GD3, since after de-O-acetyation it co-migrates with GD3. Another ganglioside co-migrated with GM2 before the alkaline treatment; however, after the treatment it was also retarded and co-migrates with GD3.
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Department of Biochemistry and Molecular Biology, University of León, Spain.
Cabezas
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D000107:Acetylation; D000818:Animals; D002855:Chromatography, Thin Layer; D005732:Gangliosides / Q000032:analysis*; D008214:Lymphocytes / Q000032:analysis*; D012794:Sialic Acids / Q000032:analysis*; D013154:Spleen / Q000276:immunology*; D013552:Swine
D005732:Gangliosides; D012794:Sialic Acids