T-cell differentiation antigen cluster 2 (CD2) is a receptor for accessory cells and can generate and/or transduce accessory signals.
The role of CD2 antigen in T-cell activation was investigated using soluble-phase or cross-linked monoclonal anti-CD2 antibodies. Soluble-phase anti-CD2 inhibited T-cell activation when accessory signals were provided with accessory cells and not when 12-0-tetradecanoyl-phorbol-13-acetate provided the accessory signals to anti-CD3-treated T cells. Soluble-phase anti-CD2 also inhibited anti-CD3 and accessory cell-dependent increases in intracellular free calcium concentration. Crosslinked anti-CD2, on the other hand, mediated an increase in the concentration of intracellular free calcium in T cells. Crosslinked anti-CD2 also functioned as an accessory cell substitute in that it generated the required accessory signals and mediated significant proliferation of anti-CD3-treated T cells. Collectively, these findings support the concept that the CD2 antigen is a receptor for accessory cells and participates in accessory signal generation and/or transduction.
Suthanthiran
M
M
Rogosin Institute, Cornell University Medical College, New York 10021.
D000911:Antibodies, Monoclonal / Q000502:physiology; D000938:Antigen-Presenting Cells / Q000276:immunology / Q000378:metabolism* / Q000502:physiology; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis* / Q000276:immunology; D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D002118:Calcium / Q000378:metabolism; D002450:Cell Communication; D006801:Humans; D007424:Intracellular Fluid / Q000378:metabolism; D008213:Lymphocyte Activation; D011971:Receptors, Immunologic / Q000502:physiology*; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism* / Q000502:physiology
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D011971:Receptors, Immunologic; D002118:Calcium