Determination of binding affinity of enantiomers to albumin by liquid chromatography.
The principles of the determination of the binding affinity constants of small molecules to albumin by liquid chromatography, using albumin as a mobile phase additive, are outlined. Chromatographic conditions for determinations of constants are presented and applied to enantiomers of tryptophan and omeprazole. The influence of albumin on the retaining properties of LiChrosorb RP-8, Phenyl Hypersil and LiChrosorb Diol was studied.
Marle
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I
Department of Analytical Pharmaceutical Chemistry, University of Uppsala, Sweden.
Pettersson
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C
Arvidsson
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T
D000327:Adsorption; D000418:Albumins / Q000032:analysis*; D002853:Chromatography, Liquid; D007202:Indicators and Reagents; D009566:Nitrates; D009853:Omeprazole / Q000032:analysis; D011485:Protein Binding; D012709:Serum Albumin / Q000032:analysis; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism; D014364:Tryptophan / Q000032:analysis
D000418:Albumins; D007202:Indicators and Reagents; D009566:Nitrates; D012709:Serum Albumin; D014364:Tryptophan; C031618:sodium nitrate; D009853:Omeprazole