Chemotactic monoclonal antibody conjugates: a comparison of four different f-Met-peptide-conjugates.
Intratumor macrophage numbers are enhanced by the administration of chemotactic f-MET-LEU-PHE - antitumor antibody - conjugates. The four f-MET-peptides f-MET-LEU-PHE, f-MET-LEU-PHE-o-methylester, formyl-norleucyl-phenylalanine and formyl-methionyl-phenylalanine, all chemotactic for human monocytes, where evaluated alone and conjugated to the melanoma directed monoclonal antibody ZME 018. f-MET-LEU-PHE-o-methyl-ester is the most active peptide, whereas f-MET-LEU-PHE - ZME 018 is the most active conjugate, not only inducing a response at the lowest concentration, but also the highest migrating cell numbers.
Obrist
R
R
Dept. of Internal Medicine of the University, Basel, Switzerland.
Schmidli
J
J
Obrecht
J P
JP
D000818:Animals; D000911:Antibodies, Monoclonal; D002633:Chemotaxis / Q000187:drug effects; D004151:Dipeptides / Q000008:administration & dosage / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D000922:Immunotoxins / Q000494:pharmacology*; D051379:Mice; D009000:Monocytes / Q000187:drug effects; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology*; D009842:Oligopeptides / Q000008:administration & dosage / Q000494:pharmacology*
D000911:Antibodies, Monoclonal; D004151:Dipeptides; D000922:Immunotoxins; D009842:Oligopeptides; C017330:N-formylmethionylphenylalanine; D009240:N-Formylmethionine Leucyl-Phenylalanine; C024998:N-formylnorleucyl-leucyl-4-phenylalanine; C042932:formylmethionyl-leucyl-phenylalanine methyl ester