Down-regulation of the T cell receptor by a mitogenic anti-Thy-1 antibody.
Antigen-specific unresponsiveness lasting at least 2 weeks can be induced in a T cell clone by 24-h pretreatment with mitogenic anti-T cell receptor antibodies. In this report the relationship is explored between the antigen-specific unresponsiveness and activation pathways triggered via the T cell receptor and Thy-1: the latter pathway is dependent on the former. A mitogenic anti-Thy-1 antibody (KT16) made the T cell clone unresponsive to specific antigen and to an anti-T cell receptor antibody coupled to Sepharose. The unresponsiveness lasted for at least 7 days. However, cells made unresponsive to specific antigen in these ways (the T cell receptor and Thy-1) could be activated by both interleukin 2 and KT16. KT16 down-modulated the T cell receptor immediately after the pretreatment, but not on day 7 after the pretreatment. These facts indicate that the state of the unresponsiveness was caused by blocking transduction of an activation signal triggered by the T cell receptor to an activation pathway shared by the T cell receptor and Thy-1.
Tomonari
K
K
Transplantation Biology Section, Clinical Research Centre, Harrow, Middlesex, GB.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000502:physiology*; D002999:Clone Cells / Q000276:immunology; D007518:Isoantibodies / Q000502:physiology*; D008213:Lymphocyte Activation; D051379:Mice; D008808:Mice, Inbred CBA; D008934:Mitogens / Q000502:physiology*; D051381:Rats; D011919:Rats, Inbred Strains; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism*; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism
D000911:Antibodies, Monoclonal; D007518:Isoantibodies; D008934:Mitogens; D011948:Receptors, Antigen, T-Cell; C035678:anti-Thy antibody