Determination of ambenonium in biological samples by reversed-phase ion-pair liquid chromatography.
A sensitive and selective analytical method for the determination of ambenonium ion in biological samples is described. The procedure involves ion-pair extraction of the drug, followed by reversed-phase ion-pair chromatographic analysis with ultraviolet detection at 217 nm. The detection limits at a signal-to-noise ratio of 5 were 100 pmol/ml using 0.2 ml of plasma and bile, 250 pmol/ml using 0.2 ml of urine and 200 pmol/g using 1 ml of tissue homogenates containing 0.1 g/ml of each tissue. This assay procedure was used to study the pharmacokinetics of ambenonium ion after intravenous administration in rats.
Yamamoto
K
K
Hospital Pharmacy, Faculty of Medicine, University of Tokyo, Japan.
Kohda
Y
Y
Saitoh
Y
Y
Nakagawa
F
F
D000549:Ambenonium Chloride / Q000097:blood / Q000378:metabolism* / Q000652:urine; D000818:Animals; D001646:Bile / Q000032:analysis / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D006863:Hydrogen-Ion Concentration; D008297:Male; D011759:Pyrrolidines; D051381:Rats; D011919:Rats, Inbred Strains; D012015:Reference Standards; D014018:Tissue Distribution
D011759:Pyrrolidines; D000549:Ambenonium Chloride; C004460:benzilonium bromide