title: The characterization of phospholipids associated with microtubules, purified tubulin and microtubule associated proteins in vitro.
abstract: 1. Significant levels of total phospholipid phosphate were detected in highly purified microtubule protein preparations. 2. While the phospholipid profiles of total microtubule proteins and microtubule-associated proteins showed both similarities and differences to that of a whole brain homogenate, purified tubulin was associated only with phospholipids that were not detectable in the latter. 3. Phosphatidyl ethanolamine, found exclusively in a fraction of microtubule associated proteins, stimulated microtubule assembly in vitro.
authors.affiliation: School of Pharmacy, Liverpool Polytechnic, England.
authors.forename: A J
authors.initials: AJ
authors.lastname: Hargreaves
authors.forename: W G
authors.initials: WG
authors.lastname: McLean
mesh_terms: D000818:Animals; D001704:Biopolymers / Q000302:isolation & purification; D001923:Brain Chemistry; D008869:Microtubule-Associated Proteins / Q000302:isolation & purification*; D008870:Microtubules / Q000032:analysis* / Q000378:metabolism; D010714:Phosphatidylethanolamines / Q000494:pharmacology; D010743:Phospholipids / Q000032:analysis* / Q000494:pharmacology; D013552:Swine; D014404:Tubulin / Q000302:isolation & purification*
chemical_list: D001704:Biopolymers; D008869:Microtubule-Associated Proteins; D010714:Phosphatidylethanolamines; D010743:Phospholipids; D014404:Tubulin