Xenobiotic metabolizing enzyme systems in test fish. III. Comparative studies of liver cytosolic glutathione S-transferases.
The reduced glutathione (GSH)-conjugating capacities of the livers of trout, carp, zebra fish, guppy, and bluegill have been evaluated with several substrates preferentially metabolized by the major GSH-transferase isozymes. Carp and zebra fish lack GSH-transferase activity with 1,2-epoxy-3-(p-nitrophenoxy)propane. Guppy is characterized by the highest activities with 1-chloro-2,4-dinitrobenzene and the absence of activity toward trans-4-phenyl-3-buten-2-one. Enzyme activities with the other substrates demonstrate quantitative interspecific differences which presumably are of minor relevance to the metabolism and toxicity of chemicals.
Donnarumma
L
L
Laboratorio di Tossicologia Comparata ed Ecotossicologia, Istituto Superiore di Sanità, Rome, Italy.
De Angelis
G
G
Gramenzi
F
F
Vittozzi
L
L
D000064:Acclimatization; D000818:Animals; D002347:Carps / Q000378:metabolism; D003600:Cytosol / Q000201:enzymology; D005399:Fishes / Q000378:metabolism*; D005978:Glutathione / Q000032:analysis; D005982:Glutathione Transferase / Q000378:metabolism*; D008099:Liver / Q000201:enzymology*; D010473:Perciformes / Q000378:metabolism; D011036:Poecilia / Q000378:metabolism; D013045:Species Specificity; D013696:Temperature; D014337:Trout / Q000378:metabolism; D015262:Xenobiotics / Q000378:metabolism*; D015027:Zebrafish / Q000378:metabolism
D015262:Xenobiotics; D005982:Glutathione Transferase; D005978:Glutathione