Central catecholamine depletion impairs in vivo immunity but not in vitro lymphocyte activation.
We have previously shown that depletion of central nervous system (CNS) catecholamines by injecting the neurotoxin 6-hydroxydopamine (6-OHDA) into the cisterna magna of C57B1/6 mice markedly impairs the humoral immune response to sheep red blood cells. This work extends these observations by showing that 6-OHDA treatment also inhibits the humoral antibody response to the T-cell-dependent antigen trinitrophenyl-keyhole limpet hemocyanin, but does not affect the response to the T-independent antigen trinitrophenyl-lipopolysaccharide. This treatment also impairs humoral responsiveness at peripheral lymphoid sites in addition to inhibiting natural killer cell activity. However, 6-OHDA treatment in vivo does not affect in vitro mixed lymphocyte responsiveness, mitogen-induced lymphocyte activation or antigen presentation by macrophages.
Cross
R J
RJ
Department of Microbiology and Immunology, University of Kentucky, Albert B. Chandler Medical Center, Lexington 40536-0084.
Roszman
T L
TL
D000818:Animals; D000917:Antibody Formation / Q000187:drug effects*; D000938:Antigen-Presenting Cells / Q000276:immunology; D000955:Antigens, T-Independent / Q000276:immunology; D002395:Catecholamines / Q000502:physiology*; D003208:Concanavalin A / Q000494:pharmacology; D006892:Hydroxydopamines / Q000494:pharmacology*; D007113:Immunity, Innate / Q000187:drug effects; D007694:Killer Cells, Natural / Q000276:immunology; D007958:Leukocyte Count; D008070:Lipopolysaccharides / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects*; D051379:Mice; D008815:Mice, Inbred Strains; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000276:immunology*
D000955:Antigens, T-Independent; D002395:Catecholamines; D006892:Hydroxydopamines; D008070:Lipopolysaccharides; D003208:Concanavalin A