Determination of digoxin in serum by on-line immunoadsorptive clean-up high-performance liquid chromatographic separation and fluorescence-reaction detection.
The use of valve-switching with an anti-digoxin immunoadsorptive clean-up cartridge prior to reversed-phase elution of serum is described. Eluted digoxin is detected by reaction of the unsplit eluent stream with hydrochloric acid in a PTFE coil, followed by fluorescence detection. Routes for optimization and evaluation of the immunoadsorptive clean-up are reported, and the effects on the recovery rate examined. The detection limit of 300 pg/ml and reproducibility of less than 7% for the application of the analytical procedure to serum are reasonably good.
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Boehringer-Mannheim GmbH, Tutzing, F.R.G.
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004077:Digoxin / Q000097:blood*; D006801:Humans; D007164:Immunosorbents; D013050:Spectrometry, Fluorescence
D007164:Immunosorbents; D004077:Digoxin