[Importance of the DNA content for the prognosis of invasive carcinoma of the bladder. Determination by photocytometry].
In a retrospective study, a DNA histogram was established from specimens obtained by cystectomy in 65 carcinomas of the bladder (stages pT1 to pT4, pN0, pN1 and pN2). Flow cytometry and automated photocytometry were systematically compared. Automated photocytometry makes it possible to differentiate between diploid, polyploid and aneuploid tumors. Among aneuploid tumors, the DNA content of tumor stem cells make it possible to identify another subgroup. The analysis of various tumor sections showed that the DNA histogram obtained by photocytometry was a stable and reproducible characteristic of the tumor. The subgroups of carcinoma of the bladder, determined by topology, have markedly different long-term prognoses. In contrast to automated photocytometry, flow cytometry did not allow for reproducible determination of the biologic characteristics of carcinoma of the bladder.
Stöckle
M
M
Urologische Klinik und Poliklinik, Johannes Gutenberg-Universität, Mainz, RFA.
Tanke
H J
HJ
Ploem
J S
JS
Hohenfellner
R
R
D003592:Cytophotometry; D004273:DNA, Neoplasm / Q000032:analysis*; D005434:Flow Cytometry; D006801:Humans; D009361:Neoplasm Invasiveness; D011003:Ploidies; D011379:Prognosis; D012189:Retrospective Studies; D001749:Urinary Bladder Neoplasms / Q000145:classification / Q000401:mortality / Q000473:pathology*
D004273:DNA, Neoplasm
Importance du contenu en ADN pour le pronostic du carcinome infiltrant de la vessie. Détermination par photocytométrie.