title: Leu-4 (CD3) antigen expression in the neoplastic cells from T-ALL and T-lymphoblastic lymphoma.
abstract: The authors studied Leu-4 (CD3) antigen localization in the neoplastic cells from six cases with T-ALL, eight with T-lymphoblastic lymphoma (T-LL), and eight with peripheral T-cell lymphomas using immunoelectron microscopy (immuno-EM). Other various monoclonal antibody studies were also performed to detect the stages of differentiation of the neoplastic cells light microscopically. The authors' study indicated that cytoplasmic Leu-4 (cLeu-4) antigen expression appeared at the early stage of thymic T-cells, together with Leu-9 (CD7) and T101 (CD5). Their immuno-EM study also disclosed that the neoplastic cells at the early stage of thymocyte differentiation all had positive results for cLeu-4 but negative results for surface Leu-4 (sLeu-4). The Leu-4 antigen expression in the neoplastic cells at the stage of common thymocyte differentiation with OKT6- (CD1) positive phenotype was variable, showing cLeu-4+sLeu-4-, cLeu-4-sLeu-4+, and cLeu-4+sLeu-4+, depending on the cases investigated. From these results, they suggest that cLeu-4 antigen appears at the stage of early thymocyte differentiation, followed by predominant sLeu-4 expression at the stage of common thymocyte differentiation, and finally simultaneous expression of cLeu-4 and sLeu-4 at the stage of peripheral T-cells.
authors.affiliation: Institute of Basic Medical Sciences, University of Tsukuba, Ibaraki, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Mori
authors.forename: K
authors.initials: K
authors.lastname: Oka
authors.forename: Y
authors.initials: Y
authors.lastname: Yoda
authors.forename: T
authors.initials: T
authors.lastname: Abe
authors.forename: M
authors.initials: M
authors.lastname: Kojima
mesh_terms: D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis*; D002462:Cell Membrane / Q000276:immunology; D003593:Cytoplasm / Q000276:immunology; D004721:Endoplasmic Reticulum / Q000276:immunology; D006801:Humans; D007150:Immunohistochemistry; D007945:Leukemia, Lymphoid / Q000276:immunology*; D008198:Lymph Nodes / Q000276:immunology; D008223:Lymphoma / Q000276:immunology*; D008854:Microscopy, Electron; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte