title: Direct effects of altered temperature on renal structure and function.
abstract: Although marked alterations in temperature often accompany ischemic, acute renal failure (ARF), the effects of altered temperature on renal structure and function have received little attention. In the present investigation, isolated rat kidneys perfused at 41 degrees C had extensive tubular damage and decreased function compared to kidneys perfused at 37 degrees C. In contrast, kidneys perfused at 30 degrees C had less tubular damage, and better function, than kidneys perfused at 37 degrees C. Increased temperature caused a 50% reduction in renal ATP (0.46 +/- 0.04 microM/100 mg tissue protein. 37 degrees C vs. 0.26 +/- 0.03 microM/100 mg tissue protein, 41 degrees C; p less than 0.05). The decreased ATP occurred despite reduced sodium reabsorption (129 +/- 8 microM/min/g, 37 degrees C vs. 65 +/- 12 microM/min/g, 41 degrees C, p less than 0.05) and normal renal oxygen consumption (QO2). These results suggest that increased temperature may cause an uncoupling of QO2 and sodium chloride transport, and an increase in nontransport mediated, basal metabolic rate may result in depleted cellular ATP levels and renal tubular cell death.
authors.affiliation: Department of Medicine, Hennepin County Medical Center, University of Minnesota, Minneapolis.
authors.forename: B L
authors.initials: BL
authors.lastname: Kasiske
authors.forename: M P
authors.initials: MP
authors.lastname: O'Donnell
authors.forename: W F
authors.initials: WF
authors.lastname: Keane
mesh_terms: D058186:Acute Kidney Injury / Q000473:pathology / Q000503:physiopathology; D000255:Adenosine Triphosphate / Q000032:analysis; D000818:Animals; D066298:In Vitro Techniques; D007511:Ischemia / Q000473:pathology / Q000503:physiopathology; D007668:Kidney / Q000098:blood supply / Q000473:pathology / Q000502:physiology*; D007677:Kidney Function Tests; D008297:Male; D010101:Oxygen Consumption; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains; D012964:Sodium / Q000378:metabolism; D013696:Temperature
chemical_list: D000255:Adenosine Triphosphate; D012964:Sodium