Ultrastructural studies of a lysosomal enzyme during lymphocyte activation.
A post-embedding immunogold technique has been used for the ultrastructural localization of a lysosomal enzyme, beta-glucuronidase, in resting and activated T- and B-lymphocytes. The results presented here show that mitogen-induced stimulation of T- and B-cells was associated with an increase in the amount of enzyme in the Golgi complex and rough endoplasmic reticulum, organelles which were rarely present in the resting lymphocytes.
Bou-Gharios
G
G
Department of Histopathology, Charing Cross and Westminster Medical School, London, UK.
Moss
J
J
Abraham
D
D
Partridge
T
T
Olsen
I
I
D000818:Animals; D001402:B-Lymphocytes / Q000201:enzymology / Q000648:ultrastructure; D004721:Endoplasmic Reticulum / Q000201:enzymology; D005966:Glucuronidase / Q000032:analysis*; D006056:Golgi Apparatus / Q000201:enzymology; D008213:Lymphocyte Activation; D051379:Mice; D008808:Mice, Inbred CBA; D008854:Microscopy, Electron; D013601:T-Lymphocytes / Q000201:enzymology / Q000648:ultrastructure
D005966:Glucuronidase