title: Determination of cytopathogenic effects of Trichomonas vaginalis on cultured cells by assaying 51Cr release.
abstract: By use of an assay for 51Cr release, it was attempted to determine the cytopathogenic effects of Trichomonas vaginalis on mammalian cultured cells. 51Cr was released from Na2(51)CrO4-labeled HeLa, L-929, FL, Vero and LLC-MK 2 cells. HeLa cells were most sensitive to the damage, followed by L-929, FL, Vero and LLC-MK 2 cells. The effect depended upon the number of parasite and the incubation period. The maximum 51Cr release from the labeled cells was observed at 37 degrees C and pH 6.0. These results attest that this assay is reliable for determining the cytopathogenic effects of T. vaginalis on the target cells.
authors.affiliation: Department of Veterinary Science, College of Agriculture, University of Osaka Prefecture, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Fukui
authors.forename: Y
authors.initials: Y
authors.lastname: Takamori
mesh_terms: D000818:Animals; D002460:Cell Line; D002860:Chromium Radioisotopes; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D013696:Temperature; D014246:Trichomonas vaginalis / Q000472:pathogenicity*; D014709:Vero Cells
chemical_list: D002860:Chromium Radioisotopes