title: Production of mouse monoclonal antibodies for the analysis of idiotypes in serum of patients with chronic lymphatic leukaemia.
abstract: In this report a simple procedure for the production of murine monoclonal antibodies (MoAb) against the idiotype of malignant B cells is described. Mice were immunized with lymphoid cells from patients with B-cell chronic lymphocytic leukaemia (B-CLL). After fusion of the spleen cells, hybridoma supernatants were screened for anti-idiotypic MoAb in ELISA with immunoglobulins obtained from tumour-cell lysates, xenohybridomas and patients' sera. The anti-idiotypic MoAb were used to study tumour cells and serum immunoglobulins (Ig) from four different patients with B-CLL. It was found that the serum IgM and IgD in one patient shared the same idiotype. Evidence is presented that IgG-secreting cell populations are not restricted to lambda-Ig-light chain-expressing B-CLL cells. With the help of anti-idiotype MoAb accurate measurements of total and idiotype-positive serum immunoglobulin levels during chemotherapy were possible.
authors.affiliation: Central Laboratory of the Netherlands Red Cross Blood Transfusion Service, Amsterdam.
authors.forename: M A
authors.initials: MA
authors.lastname: de Rie
authors.forename: D J
authors.initials: DJ
authors.lastname: van Heemstra
authors.forename: P C
authors.initials: PC
authors.lastname: Huijgens
authors.forename: W P
authors.initials: WP
authors.lastname: Zeijlemaker
authors.forename: T A
authors.initials: TA
authors.lastname: Out
authors.forename: C J
authors.initials: CJ
authors.lastname: Melief
authors.forename: A E
authors.initials: AE
authors.lastname: von dem Borne
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D001402:B-Lymphocytes / Q000276:immunology*; D006801:Humans; D007072:Immunoglobulin D / Q000276:immunology; D007130:Immunoglobulin Idiotypes / Q000032:analysis*; D007075:Immunoglobulin M / Q000276:immunology; D007945:Leukemia, Lymphoid / Q000188:drug therapy / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C
chemical_list: D000911:Antibodies, Monoclonal; D007072:Immunoglobulin D; D007130:Immunoglobulin Idiotypes; D007075:Immunoglobulin M