Evidence for two discrete phases of IL-2 production in bovine lymphocytes.
Regulation of IL-2 production in Con A-stimulated bovine lymph node cells was studied by following the time course of IL-2 synthesis and secretion over a 24-h period. A biphasic time course curve of IL-2 secretion was observed after stimulation with Con A alone or with Con A plus PMA. Both phases of IL-2 production were confirmed by three separate biochemical techniques: IL-2 protein bioassay, mRNA Northern blots, and anti-IL-2 antibody detection. Bovine IL-2 transcripts were detected as early as 2 h after Con A stimulation. The early phase of IL-2 protein secretion was initially detected 3 h after Con A stimulation, and the late phase occurred between 10 and 16 h. Both phases of IL-2 production were enhanced when PMA was added to the Con A stimulation. Each phase of IL-2 protein secretion was preceded by the accumulation of IL-2 mRNA.
Weinberg
A D
AD
Department of Microbiology, Washington State University, Pullman 99164.
Magnuson
N S
NS
Reeves
R
R
Wyatt
C R
CR
Magnuson
J A
JA
D000818:Animals; D001666:Binding Sites, Antibody; D002417:Cattle; D005434:Flow Cytometry; D007118:Immunoassay; D007376:Interleukin-2 / Q000096:biosynthesis* / Q000235:genetics; D007700:Kinetics; D008198:Lymph Nodes / Q000166:cytology; D012333:RNA, Messenger / Q000302:isolation & purification; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D013997:Time Factors; D014158:Transcription, Genetic
D007376:Interleukin-2; D012333:RNA, Messenger