Separation of alpha-amino acid enantiomers by reversed-phase high-performance liquid chromatography after derivatization with o-phthaldialdehyde and a sodium salt of 1-thio-beta-D-glucose.
A sensitive system for D,L-amino acid analysis has been developed, using fluorescence derivatization with o-phthaldialdehyde in the presence of sodium salt of 1-thio-beta-D-glucose. The reagents rapidly form fluorescent diastereoisomeric derivatives with primary amino acids. These derivatives are efficiently separated on a conventional reversed-phase column with an analysis time of 60 min. Simultaneous determination of enantiomers of various amino acids was achieved by a simple binary gradient elution with methanol in 0.05 M aqueous sodium acetate.
Jegorov
A
A
Laboratory of Analytical Chemistry, South Bohemian Biological Centre, Ceské Budĕjovice, Czechoslovakia.
Tríska
J
J
Trnka
T
T
Cerný
M
M
D000596:Amino Acids / Q000032:analysis* / Q000097:blood; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005947:Glucose / Q000031:analogs & derivatives*; D007202:Indicators and Reagents; D051379:Mice; D013237:Stereoisomerism; D009764:o-Phthalaldehyde
D000596:Amino Acids; D007202:Indicators and Reagents; D009764:o-Phthalaldehyde; C018399:1-thioglucose; D005947:Glucose