Measurement of the release of adenine nucleotides during platelet aggregation by small-bore-column isocratic high-performance liquid chromatography.
This paper describes a simple and sensitive method for measuring adenosine, adenosine monophosphate, adenosine diphosphate, and adenosine triphosphate in plasma and tissues. This method involves sample preparation by perchloric acid extraction, followed by high-performance liquid chromatography on a small-bore column with UV detection at 254 nm. The minimum detectable amounts were adenosine, 0.60 pmol; adenosine monophosphate, 0.90 pmol; adenosine diphosphate, 1.5 pmol; and adenosine triphosphate, 2.5 pmol. The interassay coefficient of variation for the measurement of these compounds was less than 4%. The influence of varying the pH and the concentration of phosphate, acetonitrile, and ion-pairing reagent are described. This method can be used to measure adenine nucleotide release during platelet aggregation.
Bodola
F
F
Department of Internal Medicine, University of Texas, Galveston 77550.
Benedict
C R
CR
D000227:Adenine Nucleotides / Q000032:analysis / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010974:Platelet Aggregation; D013056:Spectrophotometry, Ultraviolet
D000227:Adenine Nucleotides