Depletion of protein kinase C induced by an anti HLA class I monoclonal antibody in phytohemagglutinin activated human T cells.
Anti HLA Class I Monoclonal Antibody depletes Protein Kinase C (PKC) to 20% of control value in PHA activated human T cells. The effect is reversible: in 24 hours the enzymatic activity returns to 58% of control value. Removal of antibody from the culture medium increases the rate of recovery. Implications of this finding for the modulation by HLA Class I antigens of the proliferative response of T cells to lectins are discussed.
Poltronieri
L
L
Istituto di Genetica Medica, Università degli Studi di Ferrara, Italy.
Melloni
E
E
Rubini
M
M
Selvatici
R
R
Mazzilli
C
C
Baricordi
R
R
Gandini
E
E
D000328:Adult; D000911:Antibodies, Monoclonal; D002478:Cells, Cultured; D006680:HLA Antigens / Q000276:immunology*; D006801:Humans; D008213:Lymphocyte Activation; D010835:Phytohemagglutinins / Q000494:pharmacology*; D011493:Protein Kinase C / Q000378:metabolism*; D013601:T-Lymphocytes / Q000187:drug effects / Q000201:enzymology*
D000911:Antibodies, Monoclonal; D006680:HLA Antigens; D010835:Phytohemagglutinins; D011493:Protein Kinase C