title: Immunostimulatory capabilities of highly enriched Langerhans cells in vitro.
abstract: Langerhans cells (LC) are epidermal antigen-presenting cells capable of inducing allogenic, antigen-specific, and cytotoxic T cell proliferation. Previous studies have examined the dynamics of LC maintained in vitro in crude epidermal cell (EC) suspensions in which the major cell type is the keratinocyte (KC). To avoid the confounding effects of KC and other immunoregulatory cells on LC dynamics in vitro, highly enriched murine LC (85%) were studied, through 72 h of incubation in vitro, for their ability to present alloantigen (in a primary allogenic proliferation assay) and foreign antigen (in a secondary autologous proliferation assay). The results were compared to similar studies using crude EC suspensions. Freshly prepared LC are very poor stimulators of a primary allogenic proliferation response, with a 12- to 16-fold increase in stimulatory capacity by 72 h using panned-enriched and crude EC suspensions, respectively. Similarly, freshly prepared LC are weak stimulators of a secondary autologous proliferation response, with a 2.5- to 6-fold increase in immunostimulatory capability by 72 h. The overall increased stimulatory effect observed with the crude EC suspensions compared to highly enriched LC is most likely attributed to the effect of KC on T cell proliferation, rather than to a maturation effect of KC on LC during the 72 h of in vitro incubation. Using back-scattered electron imaging, the surface density of MHC-class II molecules (Ia) increased three- to fourfold through culture, which parallels the increase in functional ability. This study demonstrates that LC in either a crude or highly enriched cell suspension mature into potent immunostimulatory cells after incubation in vitro with an increased surface expression of Ia molecules. Keratinocytes are not necessary for LC maturation in vitro, but seem to exert some stimulatory effect by enhancing lymphocyte proliferation in the functional assay system.
authors.affiliation: Department of Pathology, New York State College of Veterinary Medicine, Cornell University, Ithaca 14853.
authors.forename: C A
authors.initials: CA
authors.lastname: Picut
authors.forename: C S
authors.initials: CS
authors.lastname: Lee
authors.forename: E P
authors.initials: EP
authors.lastname: Dougherty
authors.forename: K L
authors.initials: KL
authors.lastname: Anderson
authors.forename: R M
authors.initials: RM
authors.lastname: Lewis
mesh_terms: D000818:Animals; D001790:Blood Physiological Phenomena; D002478:Cells, Cultured; D004817:Epidermis / Q000502:physiology; D005260:Female; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D007114:Immunization; D007801:Langerhans Cells / Q000502:physiology*; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D000949:Histocompatibility Antigens Class II