Augmented uptake of 2-C-14-D-deoxyglucose in reversibly-injured myocardium.
We studied the cardiac uptake of 2-14C-D-deoxyglucose (14C-DG) in 6 dogs subjected to a 15 min left anterior descending coronary artery (LAD) occlusion followed by 30 min reflow. Coronary arterial flows during occlusion were determined with 46Sc microspheres. After 30 min reflow, 50 microCi 14C-DG were given intravenously to determine cardiac uptake. The animal was killed 45 min later and 46Sc counts/min were determined in gamma and beta counters. Quenching was determined with non radioactive blood for both isotopes in the beta counter. Contribution of 46Sc counts/min to the 14C channel was subtracted. 14C uptake was in DPM/g and was higher in the cardiac ischemic regions of four of the six dogs. In three of the six animals there was an inverse curvilinear relation between 14C-DG cardiac uptake and occlusion flow. This observation was further confirmed by high resolution autoradiography, indicating that enhanced cardiac uptake of radiolabeled DG may be a useful positron emission tomographic marker for reversible myocardial ischemia.
Bianco
J A
JA
Department of Nuclear Medicine, University of Massachusetts Medical Center, Worcester 01605.
Bakanauskas
J
J
Carlson
M
M
Jones
S
S
Moring
A
A
Alpert
J S
JS
Klassen
V
V
D000818:Animals; D001345:Autoradiography; D002250:Carbon Radioisotopes / Q000378:metabolism; D003326:Coronary Circulation; D003327:Coronary Disease / Q000000981:diagnostic imaging*; D003837:Deoxy Sugars / Q000378:metabolism*; D003847:Deoxyglucose / Q000378:metabolism*; D004285:Dogs; D005069:Evaluation Studies as Topic; D008863:Microspheres; D009203:Myocardial Infarction / Q000000981:diagnostic imaging; D009206:Myocardium / Q000378:metabolism*; D011817:Rabbits; D011868:Radioisotopes; D012538:Scandium; D013997:Time Factors; D014055:Tomography, Emission-Computed
D002250:Carbon Radioisotopes; D003837:Deoxy Sugars; D011868:Radioisotopes; D003847:Deoxyglucose; D012538:Scandium