Production of hepatitis B virus-infected human B-cell hybridomas: transmission of the viral genome to normal lymphocytes in cocultures.
The presence of hepatitis B virus (HBV) genome and transcripts in mononuclear cells from a patient with acute type B hepatitis offered the possibility of developing a cell line which could serve as a model for HBV replication in lymphocytes. A human B-cell hybridoma, KDG92, was then produced which carries HBV DNA in an episomal state and expresses the major virus transcripts as well as its surface (HBsAg), core (HBcAG), and e (HBeAg) antigens. KDG92 releases in the supernatant surface antigen particles but not core or Dane particles. However, in cocultures this hybridoma is able to transmit episomal HBV DNA to normal lymphocytes, both T and B cells. This in vitro system can therefore provide important indications as to the virus life cycle in lymphocytes and the mechanisms of virus propagation from cell to cell.
Colucci
G
G
Department of Medicine, Cornell University Medical College, New York, New York.
Lyons
P
P
Beazer
Y
Y
Waksal
S D
SD
D001402:B-Lymphocytes / Q000382:microbiology*; D002460:Cell Line; D004279:DNA, Viral / Q000096:biosynthesis; D006511:Hepatitis B Antigens / Q000096:biosynthesis; D006515:Hepatitis B virus / Q000302:isolation & purification* / Q000502:physiology; D006801:Humans; D006825:Hybridomas / Q000276:immunology / Q000382:microbiology*; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D010957:Plasmids; D012367:RNA, Viral / Q000096:biosynthesis; D013601:T-Lymphocytes / Q000382:microbiology*; D014776:Virus Cultivation; D014779:Virus Replication
D004279:DNA, Viral; D006511:Hepatitis B Antigens; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D012367:RNA, Viral