Generation of an anti-interleukin 2 factor in healing wounds.
Previously we have noted that fluid obtained from ten-day-old healing wounds noncytotoxically inhibits the blastogenesis of lymphocytes in response to mitogens or antigens. Since these lymphocytic responses are interleukin 2 (IL-2)-mediated, we looked for a specific IL-2 inhibitor in wound fluid. We have found that wound fluid blocks the response of thymic lymphocytes and of two cloned T-helper cell lines (D10 and HT2) to exogenous human recombinant IL-2. The wound fluid enhances fibroblast proliferation, thus demonstrating that its proliferative inhibitory activity is specific for lymphocytes. The findings suggest that wound fluid contains a factor that impairs lymphocyte response to IL-2, probably at the receptor or postreceptor level.
Breslin
R J
RJ
Department of Surgery, Sinai Hospital, Baltimore, MD 21215.
Barbul
A
A
Kupper
T S
TS
Knud-Hansen
J P
JP
Wasserkrug
H L
HL
Efron
G
G
D000818:Animals; D001826:Body Fluids / Q000032:analysis*; D005347:Fibroblasts; D008213:Lymphocyte Activation; D008222:Lymphokines / Q000032:analysis*; D008297:Male; D051381:Rats; D011917:Rats, Inbred Lew; D014945:Wound Healing; D014947:Wounds and Injuries / Q000276:immunology* / Q000503:physiopathology
D008222:Lymphokines; C046147:interleukin 2 inhibitor