title: Long-lasting thymus-independent immune responses to anti-idiotype lipopolysaccharide conjugates require continuous B cell renewal.
abstract: In the present investigation, we have studied the antibody response of both C57BL/6 lipopolysaccharide-responder mice (B6 LPS-R) and C57BL/10ScCr LPS-nonresponder mice (B10.Cr LPS-NR) upon immunization with copolymers of LPS and either Ac38 or Ac46 anti-idiotypes. As both strains of mice studied are of the Ighb allotype the antibody response obtained was quantified by estimating the serum levels of the complementary idiotype, B1-8, in immunized mice. The results show that while the injection of the LPS-R strain B6 with copolymers of LPS anti-idiotype resulted in a long-lasting idiotype response the immunization of the LPS-NR strain, B10.Cr, resulted in virtually no response. This finding allowed us to perform cell transfer experiments to study the response of isolated cells from B6 mice transferred into LPS-NR hosts. Using such a cell transfer model, the results obtained suggest that long-lasting idiotype responses observed in the LPS-R strain require continuous B cell production in bone marrow. The relevance of this finding to the understanding of the long-lasting (fluctuating) antibody responses to nondegradable thymus-independent polysaccharides is discussed.
authors.affiliation: Department of Immunology, Pasteur Institute, Paris, France.
authors.forename: O
authors.initials: O
authors.lastname: Burlen
authors.forename: A
authors.initials: A
authors.lastname: Coutinho
authors.forename: A A
authors.initials: AA
authors.lastname: Freitas
mesh_terms: D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology; D000917:Antibody Formation; D000955:Antigens, T-Independent / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology*; D001853:Bone Marrow / Q000502:physiology; D006410:Hematopoiesis; D007116:Immunization, Passive; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D007156:Immunologic Memory; D008070:Lipopolysaccharides / Q000276:immunology*; D051379:Mice; D013997:Time Factors
chemical_list: D000888:Antibodies, Anti-Idiotypic; D000955:Antigens, T-Independent; D007130:Immunoglobulin Idiotypes; D008070:Lipopolysaccharides