Influence of long-term antigenic stimulation started in young C57BL mice on the development of age-related monoclonal gammapathies.
Long-term antigenic stimulation by multiple antigens (DNP conjugated to human serum albumin (DNP-HSA), ovalbumin and pneumococcal polysaccharide) without adjuvant in young C57BL mice resulted in the development of homogeneous immunoglobulins (H-Ig) during aging in frequencies higher than those in the control group. Our data showed that antigen-specific B-cell clones were at least in part responsible for this increased incidence of age-related monoclonal gammapathies (MG). Using in situ adsorption performed on Wieme's agar plates and in immunoelectrophoresis, antibody activity to one of the immunizing agents (DNP-HSA) could be demonstrated within 10% of the in old mice appearing H-Ig components. This frequency was significantly different from the 0.3% of the H-Ig components of the aging control mice. The antigen-specific MG belonged most likely to the category of benign MG. These findings indicate that long-lasting antigenic stimulation contributes to the development of age-related B-cell proliferative disorders, namely of the benign MG.
van den Akker
T W
TW
Department of Cell Biology, Immunology and Genetics, Erasmus University, Rotterdam, The Netherlands.
Brondijk
R
R
Radl
J
J
D000375:Aging; D000818:Animals; D000937:Antigen-Antibody Reactions; D000941:Antigens / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology; D004140:Dinitrophenols / Q000276:immunology; D005260:Female; D006241:Haptens / Q000276:immunology; D006801:Humans; D007136:Immunoglobulins / Q000096:biosynthesis; D051379:Mice; D008810:Mice, Inbred C57BL; D010047:Ovalbumin / Q000276:immunology; D010265:Paraproteinemias / Q000209:etiology / Q000276:immunology* / Q000503:physiopathology; D012709:Serum Albumin / Q000276:immunology
D000941:Antigens; D004140:Dinitrophenols; D006241:Haptens; D007136:Immunoglobulins; D012709:Serum Albumin; C058064:dinitrophenyl-human serum albumin conjugate; D010047:Ovalbumin