Effects of purified human native granulocyte colony-stimulating factor (G-CSF) on proliferation of blast progenitors in acute myeloblastic leukemia.
Blast progenitors in acute myeloblastic leukemia (AML) grow in methylcellulose and suspension cultures. Blast colony formation in methylcellulose culture reflects the terminal divisions of blast progenitors, while secondary colony formation, by replating in methylcellulose and recovering clonogenic cells in suspension culture, reflects the self-renewal of blast progenitors. To analyze the regulatory mechanisms of the proliferation of leukemic blast progenitors, the effects of highly purified native granulocyte colony-stimulating factor (G-CSF) obtained from human squamous cell carcinoma line (CHU-2) on blast progenitors in AML patients were studied in methylcellulose and suspension cultures. Purified G-CSF stimulated the growth of blast progenitors in both culture systems, although sensitivity to G-CSF varied from patient to patient. No obvious maturation of leukemic blasts was noted in suspension culture in the presence of G-CSF. The data suggest that a normal hemopoietic regulator may play a role in the growth of blast progenitors in AML patients.
Nara
N
N
First Department of Internal Medicine, Tokyo Medical and Dental University, Japan.
Murohashi
I
I
Suzuki
T
T
Yamashita
Y
Y
Maruyama
Y
Y
Aoki
N
N
Tanikawa
S
S
Onozawa
Y
Y
D000328:Adult; D000368:Aged; D002471:Cell Transformation, Neoplastic / Q000187:drug effects / Q000473:pathology*; D003115:Colony-Stimulating Factors / Q000494:pharmacology*; D003470:Culture Media; D005260:Female; D006098:Granulocytes; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000473:pathology*; D008297:Male; D008747:Methylcellulose; D008875:Middle Aged; D014411:Neoplastic Stem Cells / Q000473:pathology*; D013535:Suspensions; D014410:Tumor Stem Cell Assay
D003115:Colony-Stimulating Factors; D003470:Culture Media; D013535:Suspensions; D008747:Methylcellulose