title: CpG methylation of an X-linked transgene is determined by somatic events postfertilization and not germline imprinting.
abstract: The process of X-inactivation in mammals requires at least two events, the initiation of inactivation and the maintenance of the inactive state. One possible mechanism of control is by methylation of DNA at CpG dinucleotides to maintain the inactive state. Furthermore, the paternal X-chromosome is frequently inactivated in the extraembryonic membranes. The relationship between the parental origin of the chromosome, nonrandom inactivation and DNA methylation is not clear. In this paper, we report on the CpG methylation of an X-linked transgene, CAT-32. The levels of methylation in embryonic, extraembryonic and germline cells indicates that the modifications of the transgene are broadly similar to those reported for endogenous X-linked genes. Interestingly, the methylation of CAT-32 transgene in extraembryonic tissues displays patterns that could be linked to the germline origin of each allele. Hence, the maternally derived copy of CAT-32 was relatively undermethylated when compared to the paternal one. The changes in DNA methylation were attributed to de novo methylation occurring after fertilization, most probably during differentiation of extraembryonic tissues. In order to determine whether or not the patterns of DNA methylation reflected the germline origin of the X-chromosome, we constructed triploid embryos specifically to introduce two maternal X-chromosomes in the same embryo. In some of these triploid conceptuses, methylation patterns characteristic of the paternally derived transgene were observed. This observation indicates that the methylation patterns are not necessarily dependent on the parental origin of the X-chromosome, but could be changed by somatic events after fertilization. One of the more likely mechanisms is methylation of the transgene following inactivation of the X-chromosome in extraembryonic tissues.
authors.affiliation: Department of Molecular Embryology, Institute of Animal Physiology and Genetics Research, Babraham, Cambridge, UK.
authors.forename: A
authors.initials: A
authors.lastname: Collick
authors.forename: W
authors.initials: W
authors.lastname: Reik
authors.forename: S C
authors.initials: SC
authors.lastname: Barton
authors.forename: A H
authors.initials: AH
authors.lastname: Surani
mesh_terms: D000483:Alleles; D000818:Animals; D004247:DNA / Q000378:metabolism*; D004303:Dosage Compensation, Genetic; D005260:Female; D005306:Fertilization; D005796:Genes; D008297:Male; D008745:Methylation; D051379:Mice; D008822:Mice, Transgenic
chemical_list: D004247:DNA