Generation of myc/fos transfectant Balb-3T3 cell lines.
Early and transient expression of proto-oncogenes c-fos and c-myc is involved in the mitogenic response to PDGF (platelet-derived growth factor). We used DNA-mediated transfection to approach the role played by these genes in cell growth control by PDGF and in growth deregulation (neoplasia). Cloned pFBJ-2 (v-fos) and glucocorticoid-inducible mouse c-myc were co-transfected with a neo genetic marker to allow a neutral selection on the basis of resistance to the neomycin derivative geneticin G418. pFBJ-2 transfection was found to interfere with the number of G418-resistant (G418r) colonies. By using a v-fos-deleted pFBJ-2 construct, the deleterious effect was attributed to v-fos coding sequences. Cellular fos gene disruption, by homologous recombination with exogenous v-fos, is proposed as the basis for the deleterious effect. Co-transfection with MMTV-H3-c-myc effectively counteracts the negative effects of v-fos. Different from the parental line or single myc or fos transfectants, double myc/fos transfectants are morphologically transformed. Double transfectants still retain the PDGF requirement for growth in monolayer cultures.
Armelin
M C
MC
Instituto de Química, Universidade de Sao Paulo, Brasil.
Joazeiro
C A
CA
Rocha
K M
KM
Armelin
H A
HA
D000818:Animals; D002460:Cell Line; D051379:Mice; D010982:Platelet-Derived Growth Factor / Q000502:physiology; D011519:Proto-Oncogenes; D014162:Transfection
D010982:Platelet-Derived Growth Factor