title: Specific effects of nerve growth factor on the differentiation pattern of mouse embryonic stem cells in vitro.
abstract: The effect of nerve growth factor (NGF) on the differentiation pattern of pluripotent undifferentiated mouse embryonic stem cells (ESC) was studied. Morphological analysis of differentiating stem cells and histochemical silver-staining analysis of embryonic carcinoma (P19S1801A1) and embryonic stem cells (ESC-BLC 6) permitted the demonstration of nerve cell differentiation. NGF treatment resulted in an accelerated appearance of neuron-like cells in differentiating embryoid bodies of ESC line BLC 6. By use of the silver-staining test NGF dependent induction of nerve cell lineage was demonstrated. The presented embryonic stem cell model allows to study the time course of differentiation and induction of a special cell lineage of undifferentiated embryonic stem cells by growth factors.
authors.affiliation: Zentralinstitut für Genetik und Kulturpflanzenforschung der AdW der DDR, Gatersleben.
authors.forename: A M
authors.initials: AM
authors.lastname: Wobus
authors.forename: R
authors.initials: R
authors.lastname: Grosse
authors.forename: J
authors.initials: J
authors.lastname: Schöneich
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D004622:Embryo, Mammalian; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000187:drug effects; D051379:Mice; D009132:Muscles / Q000166:cytology; D009414:Nerve Growth Factors / Q000494:pharmacology*; D009474:Neurons / Q000166:cytology; D013724:Teratoma
chemical_list: D009414:Nerve Growth Factors