Isolation of peptide hormones from the pancreas of the bullfrog (Rana catesbeiana). Amino acid sequences of pancreatic polypeptide, oxyntomodulin, and two glucagon-like peptides.
Insulin, pancreatic polypeptide, glucagon, oxyntomodulin, and two distinct glucagon-like peptides were isolated from acidic ethanol extracts of bullfrog pancreas by gel filtration followed by high pressure liquid chromatography. The amino acid sequences of pancreatic polypeptide, oxyntomodulin, and both glucagon-like peptides were determined. Frog pancreatic polypeptide contains 36 amino acid residues and has a COOH-terminal phenylalaninamide. It is more homologous with human pancreatic polypeptide (61%) than other characterized members of this family of peptides. Frog glucagon has an amino acid composition identical to the NH2-terminal 29 residues of the larger, more abundant oxyntomodulin and was not sequenced. The finding of a single form of glucagon and oxyntomodulin, but two glucagon-like peptides in frog pancreas extract is similar to that found or deduced for mammals.
Pollock
H G
HG
Department of Biochemistry, University of Kansas Medical Center, Kansas City 66103.
Hamilton
J W
JW
Rouse
J B
JB
Ebner
K E
KE
Rawitch
A B
AB
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D005260:Female; D005768:Gastrointestinal Hormones / Q000302:isolation & purification*; D005934:Glucagon / Q000302:isolation & purification*; D004763:Glucagon-Like Peptides / Q000302:isolation & purification*; D008297:Male; D008969:Molecular Sequence Data; D053772:Oxyntomodulin; D010179:Pancreas / Q000032:analysis*; D010191:Pancreatic Polypeptide / Q000302:isolation & purification*; D011892:Rana catesbeiana
D000596:Amino Acids; D005768:Gastrointestinal Hormones; D053772:Oxyntomodulin; D010191:Pancreatic Polypeptide; D004763:Glucagon-Like Peptides; D005934:Glucagon