title: Rapid miniaturized chromatography for 111In labeled monoclonal antibodies: comparison to size exclusion high performance liquid chromatography.
abstract: Our laboratory investigated the use of a rapid miniaturized chromatography system, ITLC-SG with 0.9% NaCl, to assess the radiochemical purity of 111In labeled monoclonal antibodies (MoAbs). Radiochemical analysis was performed on numerous 111In labeled antibody preparations with labeling efficiencies ranging from 40 to greater than 95% and the results compared to those obtained with size exclusion high performance liquid chromatography (HPLC). The chromatographic procedure involved challenging radiolabeled antibodies with 0.05 M DTPA to chelate unbound and/or non-specific bound 111In, spotting on miniaturized instant thin layer-silica gel chromatography strips, developing in 0.9% NaCl, and counting appropriate segments for radioactivity. Results of the study demonstrated that the miniaturized chromatography procedure was rapid, taking less than 4 min to complete, and accurate in assessing the amount of unbound or non-specific bound 111In in 111In labeled monoclonal antibodies, when compared to size exclusion HPLC.
authors.affiliation: Department of Radiology, Northwestern University Medical Center, Chicago, IL 60611.
authors.forename: A M
authors.initials: AM
authors.lastname: Zimmer
authors.forename: J M
authors.initials: JM
authors.lastname: Kazikiewicz
authors.forename: S M
authors.initials: SM
authors.lastname: Spies
authors.forename: S T
authors.initials: ST
authors.lastname: Rosen
mesh_terms: D000911:Antibodies, Monoclonal / Q000032:analysis*; D002845:Chromatography / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007205:Indium Radioisotopes / Q000032:analysis; D011786:Quality Control; D013997:Time Factors
chemical_list: D000911:Antibodies, Monoclonal; D007205:Indium Radioisotopes