The construction of the hybrid plasmid containing genes of the central part of phage T4 baseplate and gene 29 product separation.
A fragment of E. coli bacteriophage T4 genome including the four genes (genes 51, 27, 28, 29) coding for the central plug proteins was cloned into plasmid pMCC17. The genes present on this fragment were expressed in E. coli in the absence of phage infection producing hub proteins, which could be identified on polyacrylamide gels. By applying affinity chromatography protein 29 was purified from extracts of E. coli transformed with this hybrid plasmid. The isolated protein had the ability to complement T4 29 amber mutants. The molecular weight of the purified protein was estimated as 75,000 to 85,000 depending on the composition of SDS-polyacrylamide gel used for the assay.
Nieradko
J
J
Department of Microbiology, University of Gdańsk, Poland.
Szewczyk
B
B
D004591:Electrophoresis, Polyacrylamide Gel; D005814:Genes, Viral; D008970:Molecular Weight; D010957:Plasmids; D012967:Sodium Dodecyl Sulfate; D013604:T-Phages / Q000235:genetics*; D014764:Viral Proteins / Q000302:isolation & purification*
D014764:Viral Proteins; D012967:Sodium Dodecyl Sulfate