Biochemical characterization of FMRF-NH2-like peptides in spinal cords of various mammalian species using specific radioimmunoassays.
Phe-Leu-Phe-Gln-Pro-Gln-Arg-Phe-NH2 (F-8-F-NH2) and Ala-Gly-Glu-Gly-Leu-Ser-Ser-Pro-Phe-Trp-Ser-Leu-Ala-Ala-Pro-Gln-Arg-Phe-NH2 (A-18-F-NH2), originally detected by FMRF-NH2 antiserum and subsequently isolated from bovine brain, were found to be highly localized in the bovine spinal cord. Using specific radioimmunoassays coupled with HPLC, F-8-F-NH2 and A-18-F-NH2 immunoreactivities in spinal cord of bovine, rat, mouse, guinea pig and human were studied. One major F-8-F-NH2 immunoreactivity was detected in the spinal cord of every species except in human, however, the retention time of F-8-F-NH2 immunoreactivity appears to vary from species to species. In the human spinal cord three major F-8-F-NH2 immunoreactivities are detected and one of them was eluted in the position of F-8-F-NH2. Two major A-18-F-NH2 immunoreactivities were detected in every species except guinea pig; one of these immunoreactivities can be identified as F-8-F-NH2 immunoreactivity due to the high affinity of the A-18-F-NH2 antiserum to F-8-F-NH2. F-8-F-NH2 and A-18-F-NH2 immunoreactivities can also be clearly detected by FMRF-NH2 antiserum, however, the quantities of these peptides can be grossly underestimated by the FMRF-NH2 RIA. These results confirm that there is a novel system of FMRF-NH2-like peptides in mammalian CNS and some of them are more closely related to the bovine peptides, F-8-F-NH2 and A-18-F-NH2 than to FMRF-NH2.
Majane
E A
EA
Laboratory of Preclinical Pharmacology, National Institute of Mental Health, St. Elizabeths Hospital, Washington, DC 20032.
Casanova
M F
MF
Yang
H Y
HY
D000818:Animals; D002417:Cattle; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D003429:Cross Reactions; D019835:FMRFamide; D006168:Guinea Pigs; D006801:Humans; D051379:Mice; D009479:Neuropeptides / Q000032:analysis*; D011863:Radioimmunoassay / Q000379:methods; D051381:Rats; D013045:Species Specificity; D013116:Spinal Cord / Q000032:analysis*
D009479:Neuropeptides; D019835:FMRFamide