title: [The covalent-sorption reconstitution of steroid hydroxylases].
abstract: The effect of covalent immobilization via free amino groups on the catalytic activity of individual components of the cholesterol side-chain cleavage and 11b-steroid hydroxylation systems (adrenodoxin reductase, adrenodoxin, cytochrome P-450scc and cytochrome P-450(11)b) as well as on that of co-immobilized protein complexes. The protein complex formation at different stages of the monooxygenase cycle (i.e., reduction, oxygenation) was followed by direct spectrophotometric monitoring of the functional state of the immobilized complexes. Cholesterol side-chain cleavage was carried out in minicolumns, using various combinations of immobilized and soluble proteins. Cytochromes P-450scc and P-450(11)b were found to retain their functional activities after immobilization via free SH-groups.
authors.forename: V M
authors.initials: VM
authors.lastname: Shkumatov
authors.forename: V G
authors.initials: VG
authors.lastname: Radiuk
authors.forename: G I
authors.initials: GI
authors.lastname: Gaponova
authors.forename: V L
authors.initials: VL
authors.lastname: Chashchin
authors.forename: A A
authors.initials: AA
authors.lastname: Akhrem
mesh_terms: D002384:Catalysis; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D004800:Enzymes, Immobilized; D013053:Spectrophotometry; D013250:Steroid Hydroxylases / Q000378:metabolism*
chemical_list: D004800:Enzymes, Immobilized; D003577:Cytochrome P-450 Enzyme System; D013250:Steroid Hydroxylases
vernacular_title: Kovalentno-sorbtsionnaia rekonstruktsiia steroidnykh gidroksilaz.