Augmentation of pre- and post-thymic T-lymphocyte responses in thymic epithelial cell grafted nude mice.
Thymic epithelial (T-E) cell cultures from newborn mice, grown on dextran beads coated with denatured collagen, were injected subcutaneously into athymic "nude" mice. After 8 weeks, enhanced in vitro migration of T-E cell grafted nude mouse bone marrow cells occurred to thymus supernatants prepared from neonatal thymus cultures. Percent migration was equal to the migration of control AKR bone marrow. In vivo T-lymphocyte responses were also significantly enhanced in T-E cell grafted nude mice as compared to controls.
Haar
J L
JL
Department of Anatomy, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298.
Taubenberger
J K
JK
Doane
L
L
D000818:Animals; D001854:Bone Marrow Cells; D002465:Cell Movement; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D003470:Culture Media / Q000494:pharmacology; D003602:Cytotoxicity, Immunologic; D004848:Epithelium / Q000378:metabolism / Q000637:transplantation; D006412:Hematopoietic Stem Cells / Q000187:drug effects / Q000502:physiology*; D007376:Interleukin-2 / Q000096:biosynthesis; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008806:Mice, Inbred AKR; D008817:Mice, Mutant Strains; D008819:Mice, Nude; D008863:Microspheres; D013601:T-Lymphocytes / Q000276:immunology*; D013950:Thymus Gland / Q000378:metabolism / Q000637:transplantation*
D003470:Culture Media; D007376:Interleukin-2; D003208:Concanavalin A