Toxic effect of a photo-induced tryptophan-riboflavin adduct on F9 teratocarcinoma cells and preimplantation mouse embryos.
Solutions containing L-tryptophan and riboflavin exposed to visible light, under N2 atmosphere, yield a tryptophan-riboflavin adduct, able to inhibit the growth of cultured F9 teratocarcinoma cells. This same effect was found in the presence of a mixture of the tryptophan photooxidation products and the adduct, when using solutions previously irradiated with visible light in an O2 atmosphere. A cytotoxic effect was also observed with embryos incubated in the presence of a tryptophan-riboflavin adduct, in the latter case necrosis and embryo development arrest occurred.
Silva
E
E
Facultad de Química, P. Universidad Católica de Chile, Santiago.
Salim-Hanna
M
M
Becker
M I
MI
de Ioannes
A
A
D000818:Animals; D001755:Blastocyst / Q000187:drug effects* / Q000473:pathology; D008027:Light; D051379:Mice; D012256:Riboflavin / Q000528:radiation effects / Q000633:toxicity*; D013724:Teratoma / Q000473:pathology*; D014364:Tryptophan / Q000528:radiation effects / Q000633:toxicity*; D014407:Tumor Cells, Cultured
D014364:Tryptophan; D012256:Riboflavin