Primary structure of a hormonally regulated beta-glucanase of Nicotiana plumbaginifolia.
A cDNA clone for a hormonally regulated beta-glucanase from Nicotiana plumbaginifolia has been isolated by using an oligodeoxynucleotide probe, synthesized to match the previously determined N-terminal amino acid sequence. The cDNA has the complete sequence of the mature protein and contains at least part of a hydrophobic signal peptide. At the amino acid level, the beta-glucanase of N. plumbaginifolia is 73% homologous to a beta(1,3)-glucanase from tobacco and 52% homologous to a beta(1,3;1,4)-glucanase from barley. Southern-blot analysis clearly demonstrated that N. plumbaginifolia contains at least two related genes encoding beta-glucanase. The extent of the complete signal peptide of the cloned beta-glucanase was determined by sequencing part of the corresponding gene. Northern analysis showed that the expression of the beta-glucanase gene is influenced by auxins and cytokinins.
De Loose
M
M
Laboratorium voor Genetica, Rijksuniversiteit Gent, Belgium.
Alliotte
T
T
Gheysen
G
G
Genetello
C
C
Gielen
J
J
Soetaert
P
P
Van Montagu
M
M
Inzé
D
D
D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D003583:Cytokinins / Q000235:genetics*; D004247:DNA / Q000302:isolation & purification*; D005786:Gene Expression Regulation; D004693:Glucan Endo-1,3-beta-D-Glucosidase / Q000032:analysis*; D006026:Glycoside Hydrolases / Q000032:analysis*; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D010937:Plant Growth Regulators / Q000235:genetics*; D010947:Plants, Toxic; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014026:Nicotiana / Q000201:enzymology* / Q000235:genetics
D003583:Cytokinins; D015345:Oligonucleotide Probes; D010937:Plant Growth Regulators; D004247:DNA; D006026:Glycoside Hydrolases; D004693:Glucan Endo-1,3-beta-D-Glucosidase