title: Enhanced discrimination in the partition of proteins by aqueous polymer two-phase systems.
abstract: Aqueous polymer two-phase systems containing dextran T-500 and PEG 4000 can be prepared which are biphasic below 18 degrees C and monophasic at higher temperatures. Both liganded and unliganded forms of glutamate dehydrogenase and troponin, which have similar partition coefficients if the protein is added to a two-phase system at 4 degrees C, have widely differing partition coefficients if added to the same system in the monophasic state at 20 degrees C and subsequently cooled to 4 degrees C.
authors.affiliation: Department of Biochemistry, University of Sheffield, U.K.
authors.forename: M
authors.initials: M
authors.lastname: Behizad
authors.forename: T
authors.initials: T
authors.lastname: Treffry
mesh_terms: D001671:Biochemistry / Q000379:methods*; D005969:Glutamate Dehydrogenase / Q000302:isolation & purification; D011108:Polymers
chemical_list: D011108:Polymers; D005969:Glutamate Dehydrogenase