Differential expression of HLA class-I antigens on B and T lymphocytes obtained from human lymphoid tissues.
The amount of HLA class-I antigens was determined on the surface of enriched populations of B and T lymphocytes obtained from human peripheral blood, lymph nodes and spleens. The assays were performed using monoclonal antibodies that recognize different determinants on HLA class-I antigens and utilizing a simple and sensitive enzyme-linked immunosorbent assay (ELISA). Our results indicate that B lymphocytes obtained from human peripheral blood, lymph nodes and spleens express at least twice as many HLA class-I antigens as T lymphocytes obtained from the same organs.
Bishara
A
A
Department of Clinical Microbiology, Hebrew University-Hadassah Medical Center, Jerusalem, Israel.
Nelken
D
D
Brautbar
C
C
D000911:Antibodies, Monoclonal; D001402:B-Lymphocytes / Q000145:classification / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006680:HLA Antigens / Q000032:analysis* / Q000276:immunology; D006801:Humans; D008198:Lymph Nodes / Q000166:cytology; D008221:Lymphoid Tissue / Q000166:cytology*; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000145:classification / Q000276:immunology*
D000911:Antibodies, Monoclonal; D006680:HLA Antigens