title: Regulation of coenzyme A transferase and acetoacetate decarboxylase activities in Clostridium acetobutylicum.
abstract: The activity of two enzymes involved in acetone production in Clostridium acetobutylicum, acetoacetate decarboxylase and coenzyme A transferase, was studied under acidogenic or solventogenic conditions. Acetoacetate decarboxylase activity was low under acidogenic conditions and after pyruvate addition. Under the same conditions, coenzyme A transferase activity was high. A mutant which lacked acetoacetate decarboxylase activity but was positive for coenzyme A transferase activity was isolated.
authors.affiliation: Laboratoire de Chimie Biologique I, Université de Nancy 1, Vandoeuvre-lès-Nancy, France.
authors.forename: R
authors.initials: R
authors.lastname: Janati-Idrissi
authors.forename: A M
authors.initials: AM
authors.lastname: Junelles
authors.forename: H
authors.initials: H
authors.lastname: Petitdemange
authors.forename: R
authors.initials: R
authors.lastname: Gay
mesh_terms: D000096:Acetone / Q000378:metabolism; D002262:Carboxy-Lyases / Q000378:metabolism*; D003013:Clostridium / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D015256:Coenzyme A-Transferases / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D009154:Mutation; D011773:Pyruvates / Q000494:pharmacology; D019289:Pyruvic Acid
chemical_list: D011773:Pyruvates; D000096:Acetone; D019289:Pyruvic Acid; D015256:Coenzyme A-Transferases; D002262:Carboxy-Lyases; C023743:acetoacetate decarboxylase