Calcium transfer at the blood-nerve barrier of the frog sciatic nerve.
Calcium transfer across the blood-nerve barrier of the frog sciatic nerve was studied using an in situ perfusion technique and an in vivo i.v. bolus injection technique. The permeability-surface area product of 45Ca at the blood-nerve barrier, (PA)BNB, calculated from radioactivity in the desheathed nerve segment after 5 min of circulation of tracer, and corrected for the residual radioactivity in the blood space, equaled 4.4 +/- 0.4 (S.E.M.) X 10(-5) ml.s-1.g-1 wet wt. The (PA)BNB of 45Ca was independent of [Ca2+] in the perfusion medium between 0.18 and 18 mM. The permeability-surface area products of 45Ca across the perineurium [(PA)per] also was measured by an in situ incubation technique, and equaled 1.45 +/- 0.41 X 10(-5) ml.s-1.g-1 wet wt. (n = 8). The half time (t 1/2) for nerve calcium to equilibrate with plasma calcium was calculated to be 60 min. The low, passive permeability to calcium of the blood-nerve barrier probably limits marked calcium concentration changes in nerve endoneurium following transient changes of plasma calcium, but should not alter steady-state responses.
Wadhwani
K C
KC
Laboratory of Neurosciences, National Institute on Aging, Bethesda, MD 20892.
Levitan
H
H
Rapoport
S I
SI
D000818:Animals; D002118:Calcium / Q000493:pharmacokinetics*; D002132:Calcium Radioisotopes; D002199:Capillary Permeability; D005260:Female; D008433:Mathematics; D010477:Perfusion; D011894:Rana pipiens; D012584:Sciatic Nerve / Q000098:blood supply* / Q000378:metabolism; D013395:Sucrose / Q000493:pharmacokinetics
D002132:Calcium Radioisotopes; D013395:Sucrose; D002118:Calcium