Stimulation of growth of Nb2 lymphoma cells by interleukin-2 in serum-free and serum-containing media.
The human recombinant alanine-125 analogue of interleukin-2 (IL-2) causes a dose-dependent mitogenic response in rat lymphoma Nb2-11C cloned cells when tested in serum-containing medium and serum-free medium. IL-2 and hGH elicit their growth stimulation through different receptors since IL-2 does not compete with 125I-hGH for binding to Nb2 cells and Met14hGH, an antagonist of hGH, inhibits the hGH-stimulated growth of Nb2 cells but not that caused by IL-2. The tumor promoter, 12-O-tetradecanoyl phorbol-13-acetate (TPA) potentiates the action of hGH on Nb2 cells grown in both serum-containing and in serum-free medium. TPA to a variable degree also potentiates IL-2-stimulated growth of Nb2 cells when cultured in medium containing serum but has no effect on cells grown in serum-free medium. In conclusion, IL-2 is a potent mitogen for Nb2-11C cells.
Croze
F
F
Department of Physiology, Faculty of Medicine, University of Manitoba, Winnipeg, Canada.
Walker
A
A
Friesen
H G
HG
D000818:Animals; D002455:Cell Division / Q000187:drug effects*; D002460:Cell Line; D003470:Culture Media; D013006:Growth Hormone / Q000494:pharmacology; D007376:Interleukin-2 / Q000494:pharmacology*; D007700:Kinetics; D008223:Lymphoma; D051381:Rats; D011986:Receptors, Somatotropin / Q000378:metabolism
D003470:Culture Media; D007376:Interleukin-2; D011986:Receptors, Somatotropin; D013006:Growth Hormone