title: Anti-müllerian hormone and freemartinism: inhibition of germ cell development and induction of seminiferous cord-like structures in rat fetal ovaries exposed in vitro to purified bovine AMH.
abstract: In 13 and 14-day old fetal rat ovaries maintained 3 to 10 days in organ culture, purified bovine anti-Müllerian hormone (AMH) (1.5 to 3 micrograms/ml) induced a characteristic freemartin effect. Gonadal volume and germ cell number were significantly reduced, compared to control ovaries cultured in anhormonal medium, and epithelial cells with large clear cytoplasm linked by interdigitations differentiated in the gonadal blastema. These cells resembling rat fetal Sertoli cells became polarized and formed seminiferous cord-like structures delineated by a basal membrane containing laminin and fibronectin as is the case of testicular seminiferous cords at the first step of their differentiation. These data indicate that AMH is probably the testicular factor responsible for the morphological modifications of bovine freemartin gonads and suggest that this hormone could also be involved in normal morphological differentiation of the testis. In contrast, in fetal rat ovaries, AMH did not trigger the testosterone production which occurs in freemartin gonads at an early stage of the gestation.
authors.affiliation: INSERM U.293, Hôpital des Enfants-Malades, Paris, France.
authors.forename: B
authors.initials: B
authors.lastname: Vigier
authors.forename: F
authors.initials: F
authors.lastname: Watrin
authors.forename: S
authors.initials: S
authors.lastname: Magre
authors.forename: D
authors.initials: D
authors.lastname: Tran
authors.forename: O
authors.initials: O
authors.lastname: Garrigou
authors.forename: M G
authors.initials: MG
authors.lastname: Forest
authors.forename: N
authors.initials: N
authors.lastname: Josso
mesh_terms: D000818:Animals; D054304:Anti-Mullerian Hormone; D005314:Embryonic and Fetal Development / Q000187:drug effects*; D005260:Female; D005611:Freemartinism / Q000503:physiopathology*; D006023:Glycoproteins; D006131:Growth Inhibitors; D009095:Mullerian Ducts / Q000502:physiology*; D009924:Organ Culture Techniques; D010053:Ovary / Q000033:anatomy & histology / Q000187:drug effects / Q000196:embryology*; D051381:Rats; D011919:Rats, Inbred Strains; D013735:Testicular Hormones / Q000494:pharmacology*
chemical_list: D006023:Glycoproteins; D006131:Growth Inhibitors; D013735:Testicular Hormones; D054304:Anti-Mullerian Hormone