Calcitonin gene-related peptide in the bullfrog, Rana catesbeiana: localization and vascular actions.
Calcitonin gene-related peptide (CGRP) was found using radioimmunoassay in the brain and spinal cord of the bullfrog, Rana catesbeiana. The brain extracts were found to contain 241.7 +/- 68.1 pg/mg tissue, and the spinal cord contained 1753.0 +/- 96.8 pg/mg tissue. An intense immunocytochemical reaction was observed in the dorsal spinal cord. Vascular studies using helical strips of the dorsal aorta, iliac artery, and femoral artery showed CGRP to exert a vasorelaxant effect which was most pronounced in the femoral artery and minimal in the aorta. As in the rat, CGRP was shown to exert its vasorelaxant effect by inhibiting the mobilization of intracellular calcium.
Kline
L W
LW
Department of Physiology, University of Alberta, Edmonton, Canada.
Kaneko
T
T
Chiu
K W
KW
Harvey
S
S
Pang
P K
PK
D000818:Animals; D001011:Aorta / Q000187:drug effects; D001158:Arteries / Q000187:drug effects*; D001923:Brain Chemistry; D002116:Calcitonin / Q000032:analysis*; D015740:Calcitonin Gene-Related Peptide; D005263:Femoral Artery / Q000187:drug effects; D007083:Iliac Artery / Q000187:drug effects; D009479:Neuropeptides / Q000032:analysis* / Q000494:pharmacology; D011892:Rana catesbeiana; D013116:Spinal Cord / Q000032:analysis*; D014664:Vasodilation / Q000187:drug effects
D009479:Neuropeptides; D002116:Calcitonin; D015740:Calcitonin Gene-Related Peptide