title: cDNA cloning of granzyme C, a granule-associated serine protease of cytolytic T lymphocytes.
abstract: A cDNA clone corresponding to the complete amino acid sequence of a putative protease CCP2 of murine cytotoxic T lymphocytes was isolated and sequenced. The clone encodes a 248-residue long serine esterase. The deduced N-terminal amino acid sequence is identical over 40 residues to that of granzyme C, a protease of unknown function present in granules of cytotoxic lymphocytes. Analysis of the sequence of granzyme C/CCP2 reveals high homology to other granzyme proteases, i.e. granzyme A (40%) and granzyme B (67%) and to rat mast cell protease II (46%). The amino acids lining the specificity pocket are well conserved between granzyme B, C, and rat mast cell protease II, but not granzyme A, suggesting a similar general specificity of these three proteases.
authors.affiliation: Institute of Biochemistry, University of Lausanne, Switzerland.
authors.forename: D
authors.initials: D
authors.lastname: Jenne
authors.forename: C
authors.initials: C
authors.lastname: Rey
authors.forename: D
authors.initials: D
authors.lastname: Masson
authors.forename: K K
authors.initials: KK
authors.lastname: Stanley
authors.forename: J
authors.initials: J
authors.lastname: Herz
authors.forename: G
authors.initials: G
authors.lastname: Plaetinck
authors.forename: J
authors.initials: J
authors.lastname: Tschopp
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D003594:Cytoplasmic Granules / Q000201:enzymology*; D004247:DNA / Q000235:genetics; D053804:Granzymes; D008969:Molecular Sequence Data; D012697:Serine Endopeptidases / Q000235:genetics*; D013602:T-Lymphocytes, Cytotoxic / Q000201:enzymology*
chemical_list: D004247:DNA; D053804:Granzymes; C509217:Gzmc protein, rat; D012697:Serine Endopeptidases