Effects of cimetidine on prostanoid production in the gastric corpus specimens from rats.
In order to examine the effect of cimetidine on the production of prostanoids in the stomach mucosa, the amounts of prostaglandin (PG) E2, F1 alpha and thromboxane (TX) B2 were determined after the specimens from the rat stomach corpus were incubated in the presence of cimetidine. Cimetidine significantly stimulated the production of PGE2 in the specimens at a concentration of 10 microM, but did not significantly affect the production of PGF1 alpha and TXB2 at concentrations of 1 to 100 microM.
Kubota
K
K
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Science University of Tokyo, Japan.
Sugaya
K
K
Hotta
K
K
D000818:Animals; D002478:Cells, Cultured; D002927:Cimetidine / Q000494:pharmacology*; D015232:Dinoprostone / Q000096:biosynthesis; D005227:Fatty Acids / Q000096:biosynthesis*; D005753:Gastric Mucosa / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D011460:Prostaglandins F / Q000096:biosynthesis; D011466:Prostanoic Acids / Q000096:biosynthesis*; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D013929:Thromboxane B2 / Q000096:biosynthesis
D005227:Fatty Acids; D011460:Prostaglandins F; D011466:Prostanoic Acids; D013929:Thromboxane B2; D002927:Cimetidine; D015232:Dinoprostone