title: Induction in a murine tumor of immunogenic tumor variants by transfection with a foreign gene.
abstract: Transfection of the undifferentiated murine colon carcinoma line CT-26 with the gene coding for the hemagglutination antigen (HA) of influenza virus resulted in the generation of highly immunogenic tumor cells. CT-26 cells transfected with HA not only failed to grow in syngeneic mice but also protected normal animals against a challenge with otherwise lethal doses of parental nontransfected cells. The immunogenicity of HA-transfected cells appeared to correlate with surface HA expression in that tumorigenic clones of HA-transfected CT-26 cells expressed little HA, while immunogenic clones were high expressers of HA. Irradiation of immunogenic HA clones did not abrogate their immunogenicity. These observations demonstrate that immune recognition of a poorly immunogenic tumor can be produced by immunization with tumor cells expressing a defined, foreign cell surface antigen.
authors.affiliation: Oncology Center, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
authors.forename: E R
authors.initials: ER
authors.lastname: Fearon
authors.forename: T
authors.initials: T
authors.lastname: Itaya
authors.forename: B
authors.initials: B
authors.lastname: Hunt
authors.forename: B
authors.initials: B
authors.lastname: Vogelstein
authors.forename: P
authors.initials: P
authors.lastname: Frost
mesh_terms: D000818:Animals; D002460:Cell Line; D003110:Colonic Neoplasms / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D003602:Cytotoxicity, Immunologic; D005260:Female; D014644:Genetic Variation; D005822:Genetic Vectors; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D006389:Hemagglutinins, Viral / Q000235:genetics* / Q000276:immunology; D007114:Immunization; D008175:Lung Neoplasms / Q000473:pathology / Q000556:secondary; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids; D013601:T-Lymphocytes / Q000276:immunology; D014162:Transfection
chemical_list: D019267:Hemagglutinin Glycoproteins, Influenza Virus; D006389:Hemagglutinins, Viral