Characterization of alloreactive T cell hybridomas.
Four T cell hybridoma clones were established and characterized from 250 hybridoma cell lines and characterized. All clones were strongly positive for Thy-1 and H-2 antigens. The Lyt-1 and L3T4 determinants were weakly but significantly positive, whereas I-A antigens and surface immunoglobulin were negative. Three of the clones secreted IL-2 upon stimulation with H-2Kk cells, whereas the remaining clone did not. This IL-2 secretion was stimulated not only by macrophage-dendritic cells, but also by B cells. All clones produced significant footpad swelling following injection into the footpads of H-2k mice. The time course and histology of the footpad swelling suggested that the reaction might be a delayed type hypersensitivity (DTH)-like response.
Mitsuda
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Department of Pediatrics, Yokohama City University of Medicine, Japan.
D000818:Animals; D000954:Antigens, Surface; D006183:H-2 Antigens / Q000235:genetics / Q000276:immunology; D006825:Hybridomas / Q000276:immunology*; D006968:Hypersensitivity, Delayed; D007376:Interleukin-2 / Q000096:biosynthesis; D051379:Mice; D008810:Mice, Inbred C57BL; D013601:T-Lymphocytes / Q000276:immunology*
D000954:Antigens, Surface; D006183:H-2 Antigens; D007376:Interleukin-2