Use of a crude extract or a purified antigen of Trichomonas vaginalis for the detection of secretory antibodies by ELISA.
A crude aqueous extract (T2) or a gel-filtration purified antigen of T. vaginalis was utilized to detect secretory antibodies in cervico-vaginal secretions by ELISA. The test was developed under optimum conditions using a rabbit anti-Trichomonas serum. Conditions established for monitoring antibodies to trichomonas in immunized rabbits were equally effective for human secretions. The crude extract was capable of assessing secretory antibodies in 45% of women with acute trichomoniasis and 43% of healthy women. The purified antigen showed a marked antigenic activity and compared to the crude antigen, testing 6 samples from infected women, detected a false positive and reduced the O.D. value of another sample by 1/4.
Angeretti
A
A
Istituto di Microbiologia, Università di Torino, Italy.
Merlino
C
C
Savoia
D
D
Martinotti
M G
MG
D000818:Animals; D000913:Antibodies, Protozoan / Q000032:analysis*; D000953:Antigens, Protozoan / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007071:Immunoglobulin A, Secretory / Q000032:analysis*; D014247:Trichomonas Vaginitis / Q000276:immunology*; D014246:Trichomonas vaginalis / Q000276:immunology*
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D007071:Immunoglobulin A, Secretory