title: Characterization and functional properties of a novel monoclonal antibody which identifies a T cell receptor in chickens.
abstract: A monoclonal antibody, mAb6, was produced that specifically bound to chicken T lymphocytes. Immunofluorescence analysis using a fluorescence-activated cell sorter revealed that the antibody reacted with approximately 50% of blood lymphocytes and with approximately 40% of splenocytes and thymocytes. It did not react with bursal cells and erythrocytes. Among different types of hemopoietic cell lines, only a T cell line was reactive with mAb6. When coupled to Sepharose 4B beads, mAb6 was found to be highly mitogenic for chicken T cells. In soluble form, mAb6 inhibited concanavalin A-induced T cell proliferation and the cytotoxic activity of allosensitized T cells, the inhibition occurring in a dose-dependent manner for both assays. Thus, the tissue distribution and the effects of this antibody on T cell responses suggest that mAb6 recognizes a T cell receptor present on a large proportion of chicken T cells.
authors.affiliation: Institute for Animal Physiology, University of Munich, FRG.
authors.forename: J
authors.initials: J
authors.lastname: Cihak
authors.forename: H W
authors.initials: HW
authors.lastname: Ziegler-Heitbrock
authors.forename: H
authors.initials: H
authors.lastname: Trainer
authors.forename: I
authors.initials: I
authors.lastname: Schranner
authors.forename: M
authors.initials: M
authors.lastname: Merkenschlager
authors.forename: U
authors.initials: U
authors.lastname: Lösch
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000937:Antigen-Antibody Reactions; D002469:Cell Separation; D002645:Chickens / Q000276:immunology*; D003208:Concanavalin A / Q000494:pharmacology; D005434:Flow Cytometry; D006412:Hematopoietic Stem Cells / Q000276:immunology; D008213:Lymphocyte Activation; D011948:Receptors, Antigen, T-Cell / Q000276:immunology*; D013601:T-Lymphocytes / Q000145:classification / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014018:Tissue Distribution
chemical_list: D000911:Antibodies, Monoclonal; D011948:Receptors, Antigen, T-Cell; D003208:Concanavalin A