The CD4 and CD8 T cell surface antigens are associated with the internal membrane tyrosine-protein kinase p56lck.
The CD4 and CD8 T cell antigens are thought to transduce an independent signal during the process of T cell activation. We report our evaluation of the possible involvement of the lymphocyte-specific tyrosine kinase p56lck in these transduction pathways. Our data demonstrate that p56lck is specifically modulated with either CD4 or CD8 following antibody-mediated cross-linking of these molecules and that a large fraction of the total cellular lck protein can be coimmunoprecipitated with these surface glycoproteins. These results suggest that p56lck is functionally and physically associated with CD4/CD8 in normal murine T lymphocytes and support the concept that an independent signal is transduced by the interaction of these surface molecules with major histocompatibility complex determinants.
Veillette
A
A
Laboratory of Tumor Virus Biology, National Cancer Institute, Bethesda, Maryland 20892.
Bookman
M A
MA
Horak
E M
EM
Bolen
J B
JB
D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis*; D002460:Cell Line; D008285:Major Histocompatibility Complex; D051379:Mice; D011505:Protein-Tyrosine Kinases / Q000032:analysis*; D015398:Signal Transduction; D013601:T-Lymphocytes / Q000032:analysis
D000945:Antigens, Differentiation, T-Lymphocyte; D011505:Protein-Tyrosine Kinases