Inhibitor of interleukin 1 in normal human urine. Different effects on mouse thymocytes and on a murine T-cell line.
We investigated whether urine from normal individuals contains inhibitors of interleukin 1 (IL-1). Diafiltered urine from normal afebrile donors suppressed IL-1-induced interleukin 2 (IL-2) activity of mouse thymocyte supernatants. These supernatants, however, strongly suppressed IL-2-induced [3H]thymidine incorporation into the IL-2-sensitive cell line CTLL-2, whereas the urine preparations did not. This phenomenon was caused by an increased amount of thymidine secreted by the urine-treated thymocytes. Therefore, in order to prevent interference, experiments were carried out with excess [3H]thymidine. Under these circumstances, suppression of IL-1- and to a lesser extent IL-2-induced DNA synthesis was still observed, whereas the synergistic effect of IL-1 on IL-2-induced DNA synthesis was only marginally reduced. We conclude that suppression of IL-1-induced IL-2 production by mouse thymocytes is a major effect of the IL-1-inhibitory factor(s) in normal urine. When the murine EL4 cell line was used, the diafiltered urine failed to inhibit IL-1-induced IL-2 production. The detection of an IL-1 inhibitor in urine is therefore dependent on the target cells as well as the effects of IL-1 on these cells.
Svenson
M
M
Laboratory of Immunology, University Clinic for Infectious Diseases, Copenhagen, Denmark.
Bendtzen
K
K
D000818:Animals; D002460:Cell Line; D006801:Humans; D007375:Interleukin-1 / Q000037:antagonists & inhibitors*; D007376:Interleukin-2 / Q000096:biosynthesis / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D014556:Urine / Q000276:immunology*
D007375:Interleukin-1; D007376:Interleukin-2