Separation of carotenol fatty acid esters by high-performance liquid chromatography.
Employing isocratic and gradient-elution high-performance liquid chromatography (HPLC) a number of straight-chain fatty acid esters (decanoate, laurate, myristate, palmitate) of violaxanthin, auroxanthin, lutein, zeaxanthin, isozeaxanthin, and beta-cryptoxanthin, prepared by partial synthesis, have been separated on a C18 reversed-phase column. Several chromatographic conditions were developed that separated a mixture of di-fatty acid esters (dimyristate, myristate palmitate mixed ester, dipalmitate) of violaxanthin, auroxanthin, lutein, and zeaxanthin in a single chromatographic run. Hydroxycarotenoids such as lutein, zeaxanthin, and isozeaxanthin that are not easily separated by HPLC on C18 reversed-phase columns, can be readily separated after derivatization with fatty acids and chromatography of their esters. Chromatographic conditions for optimum separation of carotenoids from various classes are discussed.
Khachik
F
F
U.S. Department of Agriculture, Beltsville Human Nutrition Research Center, MD 20705.
Beecher
G R
GR
D002338:Carotenoids / Q000302:isolation & purification*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D004952:Esters / Q000302:isolation & purification; D005227:Fatty Acids / Q000302:isolation & purification*; D007202:Indicators and Reagents; D013056:Spectrophotometry, Ultraviolet
D004952:Esters; D005227:Fatty Acids; D007202:Indicators and Reagents; D002338:Carotenoids