Elimination kinetics of circulating antigens and immune complexes. III. Elimination of intravenously injected model antigens and soluble IgG immune complexes in mice.
The blood elimination kinetics of model antigens, i.e. dinitrophenyl-conjugated human serum albumin (DNP-HSA) preparations, and soluble immune complexes (IgG-anti-DNP + DNP-HSA) were analysed by the aid of a multicompartment model, allowing estimation of tissue uptake and degradation of the immune complexes (ICs). The haematocrit values remained stable after intravenous injection of the ICs in mice. Despite this fact, the blood clearance kinetics were excellently described by three exponential functions. This indicates that the rapid initial elimination of the ICs from the blood was not the result of IC-induced capillary leakage, but rather of rapid initial binding of the complexes to different tissue structures. We also present results indicating that the elimination of circulating complexes/protein aggregates is governed less by their size than by other physical/chemical properties.
Johansson
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A
Department of Medical Microbiology, University of Linköping, Sweden.
Sundqvist
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T
Skogh
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T
D000818:Animals; D000936:Antigen-Antibody Complex / Q000493:pharmacokinetics*; D004140:Dinitrophenols / Q000493:pharmacokinetics*; D007074:Immunoglobulin G / Q000493:pharmacokinetics*; D008297:Male; D008657:Metabolic Clearance Rate; D051379:Mice; D008954:Models, Biological; D012709:Serum Albumin / Q000493:pharmacokinetics*
D000936:Antigen-Antibody Complex; D004140:Dinitrophenols; D007074:Immunoglobulin G; D012709:Serum Albumin; C058064:dinitrophenyl-human serum albumin conjugate