Induction of CD43 expression during activation and terminal differentiation of human B cells.
Only a small population (25-30%) of human peripheral blood B lymphocytes expresses large sialoglycoprotein (LSGP) (CD43). However, in the presence of autologous T cells and pokeweed mitogen (PWM) a majority (50-90%) of the immunoglobulin-producing cells (cIg+ cells) that develop from these B cells express CD43 is detected with anti-CD43 monoclonal antibodies (MoAb) B1B6, and the proportion of CD43+cIg+ cells increases with time of culture. Furthermore, a relatively larger proportion (60-80%) of the IgG-producing cIg+ cells are CD43+ compared with IgM-containing cIg+ cells (30-50%). In human tonsils, significantly more CD43+ cells (35%) are found in the in vivo-activated fraction of B cells than in the fraction of resting B cells (5%). A majority of the cIg+ cells that develop from the resting or the in vivo-activated tonsillar B cells in a PWM-induced B-cell differentiation system are CD43+ (80-100%). Furthermore, tonsillar B cells depleted of CD43+ cells give rise to cIg+ cells, of which the majority are CD43+, and the proportion of such cells increases with time of culture (60-90%). Taken together, these results indicate that LSGP belongs to a group of B-cell membrane molecules that are induced and upregulated upon activation and differentiation.
Wikén
M
M
Department of Immunology, University of Stockholm, Sweden.
Björck
P
P
Axelsson
B
B
Perlmann
P
P
D015703:Antigens, CD; D000943:Antigens, Differentiation / Q000276:immunology*; D000944:Antigens, Differentiation, B-Lymphocyte / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism; D007075:Immunoglobulin M / Q000378:metabolism; D066298:In Vitro Techniques; D051917:Leukosialin; D008213:Lymphocyte Activation; D014066:Palatine Tonsil / Q000276:immunology; D011043:Pokeweed Mitogens; D011947:Receptors, Antigen, B-Cell / Q000032:analysis; D012795:Sialoglycoproteins; D013997:Time Factors
D015703:Antigens, CD; D000943:Antigens, Differentiation; D000944:Antigens, Differentiation, B-Lymphocyte; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D051917:Leukosialin; D011043:Pokeweed Mitogens; D011947:Receptors, Antigen, B-Cell; C465014:SPN protein, human; D012795:Sialoglycoproteins