[3H]inositol 1,4,5-triphosphate binding in human cerebral cortex.
Specific, saturable and reversible binding of [3H]inositol 1,4,5-triphosphate (IP3) was demonstrated to membranes prepared from autopsied cerebral cortices from 6 subjects who were free from psychiatric or neurological disease. The binding has an affinity of 27 +/- 8 nM (mean +/- S.E.M.); capacity (Bmax) of 1.09 +/- 0.18 pmol/mg protein and is reversible in the presence of an excess of unlabelled IP3. These [3H]IP3 binding sites are likely to be physiologically significant receptors which merit further characterization in the normal and diseased human brain.
Young
L T
LT
Department of Psychiatry, University of Toronto, Ont., Canada.
Li
P P
PP
Kish
S J
SJ
Chiu
A S
AS
Warsh
J J
JJ
D000293:Adolescent; D000328:Adult; D000368:Aged; D001667:Binding, Competitive; D002540:Cerebral Cortex / Q000378:metabolism*; D006801:Humans; D015544:Inositol 1,4,5-Trisphosphate; D007295:Inositol Phosphates / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D008875:Middle Aged; D013347:Subcellular Fractions / Q000378:metabolism; D013403:Sugar Phosphates / Q000378:metabolism*
D007295:Inositol Phosphates; D013403:Sugar Phosphates; D015544:Inositol 1,4,5-Trisphosphate