Isozymes of alpha-amylase in the porcine pancreas: population distribution.
1. Three isozymes of pancreatic alpha-amylase, PPA 1, PPA 2, and PPA 3, were observed in a porcine population of 50 animals. 2. Isozyme PPA 2 was common to each pancreas. 3. Three phenotypic patterns were described as: (A) consisting of PPA 2 alone (20%); (B) consisting of PPA 1 and PPA 2 (78%); and (C) consisting of all three forms (2%). 4. Amylase isozymes were separated by anion exchange chromatography using DE53. 5. Individual isozymes corresponded to one of the three isozymes found in pancreatin. 6. Individual isozymes were inhibited equally by an amylase inhibitor from wheat. 7. Differences in amylase isozymes were attributed to genetically controlled mechanisms and not to artifacts of isolation.
Strumeyer
D H
DH
Department of Biochemistry and Microbiology, Rutgers, State University of New Jersey, New Brunswick 08903.
Kao
W
W
Roberts
T
T
Forsyth-Davis
D
D
D000818:Animals; D002852:Chromatography, Ion Exchange; D005828:Genetics, Population; D007527:Isoenzymes / Q000235:genetics / Q000302:isolation & purification*; D010179:Pancreas / Q000201:enzymology*; D011110:Polymorphism, Genetic; D013552:Swine / Q000235:genetics / Q000378:metabolism*; D000516:alpha-Amylases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000302:isolation & purification*
D007527:Isoenzymes; D000516:alpha-Amylases