Liquid chromatographic determination of sulfamethazine in feeds.
A reverse-phase liquid chromatographic method for the assay of sulfamethazine (SMZ) in feeds is described. Feed samples are extracted with 50% methanol solution, centrifuged, filtered, and diluted when necessary, and chromatographed on a C-18 column. Samples are eluted with a mobile phase of 20% methanol and 80% of a solution containing acetic acid and tetramethylammonium chloride. The average recovery from spiked samples was 97.2% with a coefficient of variation of 1.2%. Linearity was very good (correlation coefficient 0.9997). Within-day and between-day coefficients of variation averaged 1.3 and 2.6%, respectively. The results for samples assayed by this method compared closely with the results from the same extracts assayed by the AOAC colorimetric method.
Houglum
J E
JE
South Dakota State University, Chemistry Department, Brookings.
Larson
R D
RD
Neal
R M
RM
D000821:Animal Feed / Q000032:analysis*; D002853:Chromatography, Liquid; D002855:Chromatography, Thin Layer; D003124:Colorimetry; D013418:Sulfamethazine / Q000032:analysis*
D013418:Sulfamethazine