Comparison of whole-cell protein electrophoretic profiles of Haemophilus influenzae: implementation of a microcomputer mainframe linked system and description of a new similarity coefficient.
A microcomputer mainframe linked system is described which allows video camera data capture and storage of one-dimensional whole-cell protein electrophoresis gel images, processing of normalized traces to produce a similarity matrix, and analysis of the matrix using the commercial cluster analysis program CLUSTAN. A new similarity coefficient is introduced which takes into account both band position and intensity. Forty-five strains of Haemophilus influenzae, including the eight biotypes and six serotypes, were analyzed using this system. Results demonstrated groupings which are consistent with known genetic relationships.
Albritton
W L
WL
Department of Microbiology, University of Saskatchewan, Saskatoon, Canada.
Chen
X P
XP
Khanna
V
V
D001426:Bacterial Proteins / Q000302:isolation & purification*; D003201:Computers; D003204:Computers, Mainframe; D004591:Electrophoresis, Polyacrylamide Gel; D006193:Haemophilus influenzae / Q000032:analysis* / Q000145:classification; D008838:Microcomputers; D012703:Serotyping; D012967:Sodium Dodecyl Sulfate; D014743:Videotape Recording
D001426:Bacterial Proteins; D012967:Sodium Dodecyl Sulfate