Interleukin-1 alpha and beta induce interleukin-1 beta gene expression in human dermal fibroblasts.
The ability of the two forms of interleukin-1, IL-1 alpha and IL-1 beta, to induce IL-1 beta gene expression in human skin fibroblasts was studied in vitro, using Northern blot hybridization. Both recombinant IL-1 alpha and IL-1 beta caused a dramatic increase in IL-1 beta mRNA levels, IL-1 alpha being more efficient than IL-1 beta. Blockage of the prostaglandin synthesis by indomethacin reduced the basal level of IL-1 beta mRNA in control cultures and decreased also the stimulatory effect exerted by both IL-1s on IL-1 beta gene expression. These data suggest that IL-1 and prostaglandin (mainly PGE2) may act synergistically to stimulate IL-1 gene expression in dermal fibroblasts, contributing as a local amplifier system to the alterations of connective tissue in inflammatory processes.
Mauviel
A
A
Laboratoire de Biochimie du Tissu Conjonctif, C.H.U. Côte de Nacre, Caen, France.
Temime
N
N
Charron
D
D
Loyau
G
G
Pujol
J P
JP
D002478:Cells, Cultured; D015232:Dinoprostone / Q000096:biosynthesis; D004357:Drug Synergism; D005347:Fibroblasts / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology; D007375:Interleukin-1 / Q000096:biosynthesis / Q000235:genetics / Q000494:pharmacology*; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism*; D011994:Recombinant Proteins / Q000494:pharmacology; D012867:Skin / Q000378:metabolism*
D007375:Interleukin-1; D012333:RNA, Messenger; D011994:Recombinant Proteins; D015232:Dinoprostone; D007213:Indomethacin