title: Evidence for two discrete phases of IL-2 production in bovine lymphocytes.
abstract: Regulation of IL-2 production in Con A-stimulated bovine lymph node cells was studied by following the time course of IL-2 synthesis and secretion over a 24-h period. A biphasic time course curve of IL-2 secretion was observed after stimulation with Con A alone or with Con A plus PMA. Both phases of IL-2 production were confirmed by three separate biochemical techniques: IL-2 protein bioassay, mRNA Northern blots, and anti-IL-2 antibody detection. Bovine IL-2 transcripts were detected as early as 2 h after Con A stimulation. The early phase of IL-2 protein secretion was initially detected 3 h after Con A stimulation, and the late phase occurred between 10 and 16 h. Both phases of IL-2 production were enhanced when PMA was added to the Con A stimulation. Each phase of IL-2 protein secretion was preceded by the accumulation of IL-2 mRNA.
authors.affiliation: Department of Microbiology, Washington State University, Pullman 99164.
authors.forename: A D
authors.initials: AD
authors.lastname: Weinberg
authors.forename: N S
authors.initials: NS
authors.lastname: Magnuson
authors.forename: R
authors.initials: R
authors.lastname: Reeves
authors.forename: C R
authors.initials: CR
authors.lastname: Wyatt
authors.forename: J A
authors.initials: JA
authors.lastname: Magnuson
mesh_terms: D000818:Animals; D001666:Binding Sites, Antibody; D002417:Cattle; D005434:Flow Cytometry; D007118:Immunoassay; D007376:Interleukin-2 / Q000096:biosynthesis* / Q000235:genetics; D007700:Kinetics; D008198:Lymph Nodes / Q000166:cytology; D012333:RNA, Messenger / Q000302:isolation & purification; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D013997:Time Factors; D014158:Transcription, Genetic
chemical_list: D007376:Interleukin-2; D012333:RNA, Messenger