A dimeric inhibitor or insect alpha-amylase from barley. Cloning of the cDNA and identification of the protein.
A cDNA clone, designated pUP-44, whose longest open reading frame codes for a protein that is homologous to the wheat alpha-amylase inhibitors, has been isolated from a library obtained from developing barley endosperm. The deduced sequence for the mature protein, which is 122 residues long, is preceded by a sequence of 30 residues which has the typical features of a signal peptide. A closely corresponding protein, designated BDAI-1, has been isolated from mature endosperm. BDAI-1 behaves as a dimer and inhibits the alpha-amylase from the insect Tenebrio molitor at concentrations that have no effect on salivary or pancreatic alpha-amylases.
Lázaro
A
A
Departamento de Bioquímica, Escuela Técnica Superior de Ingenieros Agrónomos, Universidad Politécnica, Madrid, Spain.
Sanchez-Monge
R
R
Salcedo
G
G
Paz-Ares
J
J
Carbonero
P
P
García-Olmedo
F
F
D000818:Animals; D001483:Base Sequence; D002850:Chromatography, Gel; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis*; D002523:Edible Grain / Q000201:enzymology*; D004791:Enzyme Inhibitors; D001467:Hordeum / Q000201:enzymology*; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000032:analysis*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000032:analysis; D012689:Sequence Homology, Nucleic Acid; D013712:Tenebrio / Q000201:enzymology; D014908:Triticum / Q000201:enzymology; D014361:Trypsin Inhibitors / Q000032:analysis; D000516:alpha-Amylases / Q000037:antagonists & inhibitors*
D004791:Enzyme Inhibitors; D010940:Plant Proteins; D012333:RNA, Messenger; D014361:Trypsin Inhibitors; D004247:DNA; D000516:alpha-Amylases