Extraadrenal immunohistochemical distribution of steroid 21-hydroxylase in human.
Using an antibody against cytochrome P-450 specific for steroid 21-hydroxylase (P-450C21), extraadrenal distribution and localization of the enzyme were immunohistochemically examined on various normal human tissues. Immunoreactive P-450C21 was demonstrated in the renal tubular system, especially in distal tubules and cortical and medullary collecting tubules; excretory ducts including intercalated ducts of the pancreas and salivary glands; secretory ducts of the mammary glands. The immunoreactivity for P-450C21 was not present in the colon and liver. Transepithelial transport of sodium, potassium and hydrogen ions occurs in those sites where P-450C21 is present and mineralocorticoid receptors are presumed to be present. Relationship of extraadrenal 21-hydroxylase, which produces deoxycorticosterone locally, to a mineralocorticoid action is proposed.
Sasano
H
H
Department of Pathology, Tohoku University School of Medicine, Sendai, Japan.
Sasano
N
N
D001940:Breast / Q000201:enzymology; D003106:Colon / Q000201:enzymology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007668:Kidney / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D010179:Pancreas / Q000201:enzymology; D012469:Salivary Glands / Q000201:enzymology; D013255:Steroid 21-Hydroxylase / Q000032:analysis*; D013250:Steroid Hydroxylases / Q000032:analysis*
D013250:Steroid Hydroxylases; D013255:Steroid 21-Hydroxylase