Selective inhibition of antigen presentation to cloned T cells by protease inhibitors.
The effect of eight microbial protease inhibitors on Ag-presentation to six different Ag-specific T cell clones was investigated. We found that these protease inhibitors can inhibit Ag presentation in a highly selective manner. This selectivity was evident with T cell clones specific to different Ag as well as with T cells specific to the same Ag but differing in their H-2 restriction. The inhibition was to due to cytotoxicity or effects through the TCR because none of the eight inhibitors inhibited IL-2-induced T cell proliferation, and because they did not inhibit Ag presentation by fixed APC or synthetic polypeptide. The conclusion after these data suggests that each specific antigenic fragment is produced by a unique set of proteases.
Puri
J
J
Department of Chemical Immunology, Weizmann Institute of Sciences, Rehovot, Israel.
Factorovich
Y
Y
D000818:Animals; D000938:Antigen-Presenting Cells / Q000187:drug effects*; D000976:Antipain / Q000494:pharmacology; D002999:Clone Cells / Q000145:classification / Q000187:drug effects; D000949:Histocompatibility Antigens Class II; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D007976:Leupeptins / Q000494:pharmacology; D008666:Metalloendopeptidases / Q000037:antagonists & inhibitors; D051379:Mice; D009842:Oligopeptides / Q000494:pharmacology; D011480:Protease Inhibitors / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000145:classification / Q000187:drug effects*
D000949:Histocompatibility Antigens Class II; D007166:Immunosuppressive Agents; D007976:Leupeptins; D009842:Oligopeptides; D011480:Protease Inhibitors; D000976:Antipain; C002101:chymostatin; D008666:Metalloendopeptidases; C032854:leupeptin