Deficient phytohemagglutinin-induced interleukin-2 activity in patients with inactive systemic lupus erythematosus is correctable by the addition of phorbol myristate acetate.
In systemic lupus erythematosus (SLE) patients, the production of interleukin-2 (IL-2) by blood T lymphocytes in response to stimulation with phytohemagglutinin (PHA) either alone or with phorbol myristate acetate (PMA) or ionomycin, a Ca2+ ionophore, was examined. Deficiency in PHA-stimulated IL-2 production by cells from SLE patients was repaired by the addition of PMA, but not ionomycin. PMA alone did not stimulate IL-2 production but, in concert with PHA, induced IL-2 synthesis. Moreover, PMA was effective in the repair of the deficiency of PHA-induced IL-2 production by both T4+ and T8+ subsets. Thus, for effective IL-2 production, SLE T cells required signals either distinct from or in addition to those supplied by PHA.
Murakawa
Y
Y
Department of Internal Medicine, Shimane Medical University, Japan.
Sakane
T
T
D000328:Adult; D001696:Biomechanical Phenomena; D004987:Ethers / Q000494:pharmacology; D005260:Female; D006801:Humans; D007376:Interleukin-2 / Q000096:biosynthesis / Q000172:deficiency* / Q000378:metabolism; D015759:Ionomycin; D008180:Lupus Erythematosus, Systemic / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D010835:Phytohemagglutinins / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000145:classification / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D004987:Ethers; D007376:Interleukin-2; D010835:Phytohemagglutinins; D015759:Ionomycin; D013755:Tetradecanoylphorbol Acetate