Determination of thiamphenicol in bovine plasma by liquid chromatography.
A liquid chromatographic method is described for the measurement of thiamphenicol in bovine plasma. The plasma (1 mL) is extracted with ethyl acetate. After the solvent is evaporated under a stream of nitrogen, the residue is reconstituted in methanol-water and analyzed by reverse-phase liquid chromatography with UV detection at 224 nm. The intra-day recoveries for bovine plasma spiked with 5 and 50 micrograms/mL of thiamphenicol were 102 and 101%, respectively, with coefficients of variation of 2.40 and 0.28%, respectively. The interday recoveries for the 5 and 50 micrograms/mL samples were 103 and 101%, respectively, with coefficients of variation of 3.40 and 0.94%, respectively. The sensitivity of the method allows quantitation to at least the 100 ng/mL level.
Felice
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University of Minnesota, College of Veterinary Medicine, Department of Veterinary Diagnostic Investigation, St. Paul, 55108.
Abdennebi
E H
EH
Ashraf
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M
D000818:Animals; D002417:Cattle; D002853:Chromatography, Liquid; D007202:Indicators and Reagents; D012015:Reference Standards; D013839:Thiamphenicol / Q000097:blood*
D007202:Indicators and Reagents; D013839:Thiamphenicol