Epidermal-growth-factor stimulation of gluconeogenesis in isolated rat hepatocytes involves the inactivation of pyruvate kinase.
Preincubation of rat hepatocytes with EGF (epidermal growth factor) caused a stimulation of gluconeogenesis from alanine. The effect was maximal after preincubation of 20 min, and a half-maximal effect of EGF was obtained at 10 nM. EGF also stimulated gluconeogenesis from lactate and asparagine, but not from glutamine or from proline. Preincubation of hepatocytes with EGF caused a stable inactivation of pyruvate kinase, which may account, at least in part, for the observed effects of EGF on gluconeogenesis.
Moule
S K
SK
Department of Biochemistry, School of Medical Sciences, University Walk, Bristol, U.K.
McGivan
J F
JF
D000818:Animals; D004305:Dose-Response Relationship, Drug; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D005943:Gluconeogenesis / Q000187:drug effects*; D066298:In Vitro Techniques; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D011770:Pyruvate Kinase / Q000037:antagonists & inhibitors*; D051381:Rats; D011919:Rats, Inbred Strains; D013268:Stimulation, Chemical
D004815:Epidermal Growth Factor; D011770:Pyruvate Kinase