Control of IGF-II mRNA levels by glucocorticoids in the neonatal rat.
Insulin-like growth factor-II (IGF-II) is thought to be a major growth factor during fetal and neonatal development. Levels of IGF-II mRNA fall dramatically in the liver - the major site of endocrine production - between 18 and 20 days post-natally. No information concerning the control of gene expression post-natally has hitherto been available. Using Northern blotting and in-situ hybridization, we show here that cortisone acetate rapidly extinguishes IGF-II mRNA expression in the neonatal rat liver. The effect at putative autocrine/paracrine locations such as skeletal muscle and choroid plexus is much less marked or absent. The repression by cortisone acetate is discussed in the light of the available IGF-II gene sequence.
Beck
F
F
Department of Anatomy, University of Leicester.
Samani
N J
NJ
Senior
P
P
Byrne
S
S
Morgan
K
K
Gebhard
R
R
Brammar
W J
WJ
D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism*; D015152:Blotting, Northern; D002831:Choroid Plexus / Q000378:metabolism; D003348:Cortisone / Q000031:analogs & derivatives* / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects*; D007335:Insulin-Like Growth Factor II / Q000235:genetics*; D008099:Liver / Q000378:metabolism; D009132:Muscles / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000502:physiology; D051381:Rats; D013002:Somatomedins / Q000235:genetics*
D012333:RNA, Messenger; D013002:Somatomedins; D007335:Insulin-Like Growth Factor II; D003348:Cortisone