Effects of ATP synthesis inhibitors, temperature, 8-bromo c-AMP, and calcium omission on release of corticotropin releasing factor from superfused rat hypothalami.
The release of immunoreactive corticotropin-releasing factor (I-CRF) was studied in vitro using a hypothalamic superfusion system. Omission of calcium, or inclusion of ATP synthesis inhibitors in the medium significantly reduced the rates of basal and K+-induced I-CRF release. Reducing the incubation temperature of the hypothalami to 4 degrees C caused a reversible inhibition of basal I-CRF release. I-CRF release was stimulated in a dose-dependent fashion by 8-bromo c-AMP. Those results suggest that the in vitro release of CRF is energy-dependent and may involve a calcium and c-AMP-mediated mechanism.
Turkelson
C M
CM
Department of Physiology, University of Kansas Medical Center, Kansas City.
D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000502:physiology*; D000818:Animals; D002118:Calcium / Q000502:physiology; D003346:Corticotropin-Releasing Hormone / Q000378:metabolism*; D007031:Hypothalamus / Q000378:metabolism*; D066298:In Vitro Techniques; D007461:Iodoacetates / Q000494:pharmacology; D019807:Iodoacetic Acid; D008297:Male; D010477:Perfusion; D011188:Potassium / Q000502:physiology; D051381:Rats; D012636:Secretory Rate / Q000187:drug effects; D012966:Sodium Cyanide / Q000494:pharmacology; D013696:Temperature
D007461:Iodoacetates; D015124:8-Bromo Cyclic Adenosine Monophosphate; D000255:Adenosine Triphosphate; D003346:Corticotropin-Releasing Hormone; D012966:Sodium Cyanide; D011188:Potassium; D002118:Calcium; D019807:Iodoacetic Acid