A novel method for nucleic acid sequence determination.
We describe a novel sequencing methodology which should be readily and completely automated. The method relies on fragmentation of a nucleotide or deoxynucleotide sequence into short fragments, and subsequent quantitation of the fragments by hybridization to oligo-deoxynucleotides on a solid support. The original sequence may be reconstructed from the resulting table of fragment frequencies. We present a specific protocol which would allow practical implementation of this approach.
Bains
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Department of Biochemistry, University of Bath, U.K.
Smith
G C
GC
D001483:Base Sequence; D008722:Methods; D009693:Nucleic Acid Hybridization; D012091:Repetitive Sequences, Nucleic Acid