Effect of 4-hydroxyandrostenedione on 19-hydroxylation of deoxycorticosterone in the golden Syrian hamster.
4-Hydroxyandrostenedione, a known inhibitor of ovarian and peripheral aromatization of androgen (testosterone and androstenedione) to form estrogen, was studied for its inhibitory effect on the 19-hydroxylating enzyme system of the adrenal for the conversion of deoxycorticosterone to 19-hydroxydeoxycorticosterone. In vitro incubation of Golden Syrian hamster adrenal homogenates with tritiated deoxycorticosterone demonstrated (80-85%) reduction in label incorporated into 19-hydroxydeoxycorticosterone in the presence of 4-hydroxyandrostenedione.
Dale
S L
SL
Evans Memorial Department of Clinical Research, University Hospital, Boston University Medical Center, MA 02118.
Holbrook
M M
MM
Brodie
A M
AM
Melby
J C
JC
D000311:Adrenal Glands / Q000201:enzymology; D000735:Androstenedione / Q000031:analogs & derivatives* / Q000494:pharmacology; D000818:Animals; D006224:Cricetinae; D003900:Desoxycorticosterone / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000378:metabolism*; D008647:Mesocricetus; D006899:Mixed Function Oxygenases / Q000037:antagonists & inhibitors
C027855:19-hydroxydeoxycorticosterone; D000735:Androstenedione; D003900:Desoxycorticosterone; D006899:Mixed Function Oxygenases; C014594:formestane