T4 endocytosis and phosphorylation induced by phorbol esters but not by mitogen or HIV infection.
The T4 (CD4) molecule has been shown to facilitate the interactions of T cells with HLA class II determinants, to function as a signal transducing molecule, and to serve as a receptor for HIV. Recent studies demonstrated that both phorbol esters and antigen stimulation induced the rapid and transient modulation and phosphorylation of T4 on an IL-2-dependent line of cloned peripheral blood T4+ cells. In the current study, we define the kinetics of T4 phosphorylation and internalization induced by phorbol esters and determine the extent to which this metabolic pathway is required for T cell proliferation, activation, and HIV infection. On both peripheral blood T4+ cells and the T cell line Sup-T1, the modulation and internalization of surface T4 induced by phorbol 12, 13-dibutyrate (PDB) was preceded by rapid and transient phosphorylation. On both cell types, by 48 h, T4 was reexpressed on the cell surface in a nonphosphorylated form and was shown to be resistant to phosphorylation and internalization when these cells were reexposed to PDB. In contrast, T4 on the surface of PBL was neither phosphorylated nor down-modulated when PBL were stimulated by PHA, indicating that these effects were not simply the result of T cell activation or proliferation. In additional studies, we demonstrate that this pathway for T4 phosphorylation and internalization is not required for HIV infection by showing that 1) the binding of the HIV gp 120 envelope to T4 does not induce phosphorylation of T4, 2) Sup-T1 cells that are rendered resistant to phorbol ester-induced T4 internalization and phosphorylation by prolonged culture in PDB remain highly susceptible to HIV infection, and 3) clones of HIV-producing cells expressing high levels of surface T4 that is complexed with viral envelope remain susceptible to PDB-induced modulation of T4. This observation suggests that, at least on lymphoid cells, HIV penetration does not occur exclusively by R-mediated endocytosis.
Hoxie
J A
JA
Hematology-Oncology Section, Hospital of the University of Pennsylvania, Philadelphia 19104.
Rackowski
J L
JL
Haggarty
B S
BS
Gaulton
G N
GN
D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology* / Q000378:metabolism; D000328:Adult; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis* / Q000378:metabolism; D004705:Endocytosis / Q000187:drug effects*; D006801:Humans; D007700:Kinetics; D008213:Lymphocyte Activation / Q000187:drug effects*; D015240:Phorbol 12,13-Dibutyrate; D010703:Phorbol Esters / Q000494:pharmacology*; D010766:Phosphorylation; D010835:Phytohemagglutinins / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism
D000945:Antigens, Differentiation, T-Lymphocyte; D010703:Phorbol Esters; D010835:Phytohemagglutinins; D015240:Phorbol 12,13-Dibutyrate