title: Analysis of the genetic polymorphism of coagulation factor XIIIB (FXIIIB) by isoelectric focusing.
abstract: The genetic variants of the coagulation factor XIIIB (FXIIIB) were analyzed by isoelectric focusing, carried out in agarose gels and followed by immunofixation. The FXIIIB phenotypes were visualized by a combined staining procedure with Coomassie Brilliant Blue R-250 and silver nitrate. Improved resolution was accomplished in polyacrylamide gels by hybrid isoelectric focusing in immobilized pH gradients supplemented with carrier ampholytes. We examined a total of 1,604 unrelated, healthy individuals from Southern Germany. The frequencies for the FXIIIB alleles were B*1 = 0.7581, B*2 = 0.0843, B*3 = 0.1568 and B*4 = 0.0019. The theoretical exclusion rate for disputed paternity is 22.35%.
authors.affiliation: Blood Transfusion Service, Bavarian Red Cross, Munich, Federal Republic of Germany.
authors.forename: H J
authors.initials: HJ
authors.lastname: Leifheit
authors.forename: H
authors.initials: H
authors.lastname: Cleve
mesh_terms: D000665:Ampholyte Mixtures; D005176:Factor XIII / Q000032:analysis / Q000235:genetics*; D006801:Humans; D007525:Isoelectric Focusing; D010641:Phenotype; D011110:Polymorphism, Genetic
chemical_list: D000665:Ampholyte Mixtures; D005176:Factor XIII; C054380:factor XIIIb