Culture of in vitro fertilized rabbit ova.
Embryonic development of in-vitro fertilized rabbit ova was assessed following in-vitro culture in four different serum supplemented media. A mixture of Basal Medium Eagle (BME) and Ham's F10 medium (1:1) provided better support for in-vitro development than Ham's F10, BME, or regular acidic saline (RAS). In-vitro embryonic development in the BME/Ham's F10 mixture was synchronous with in-vivo development through at least 55 hr of culture. After 54 hr of culture, embryos transferred to the oviduct of a synchronous pseudopregnant recipient were able to implant at the same rate as simultaneously transferred embryos grown in vivo. BME/Ham's F10 supplemented with 10% newborn calf serum was highly supportive of rabbit embryo development following in-vitro fertilization.
Keefer
C L
CL
Department of Physiology and Pharmacology, University of Georgia College of Veterinary Medicine, Athens 30602.
Fayrer-Hosken
R A
RA
Brown
L M
LM
Brackett
B G
BG
D000818:Animals; D002478:Cells, Cultured; D003470:Culture Media; D004622:Embryo, Mammalian / Q000502:physiology*; D005260:Female; D005307:Fertilization in Vitro; D008297:Male; D011817:Rabbits; D015053:Zygote / Q000502:physiology*
D003470:Culture Media