In vivo formation of omega-oxidized metabolites of leukotriene C4 in the rat.
[3H8]Leukotriene C4 was administered to germfree rats and to conventional rats having a bile duct cannula. Several radioactive metabolites were isolated. Two polar biliary metabolites from conventional rats were identified as N-acetyl-omega-carboxy-leukotriene E4 and N-acetyl-omega-hydroxy-leukotriene E4. A polar fecal metabolite from germfree rats was found to be N-acetyl-omega-carboxy-leukotriene E4. Chemical identities were established using UV spectroscopy and cochromatographies with authentic compounds in several HPLC systems. The fecal metabolite was further characterized by reductive desulfurization followed by gas-liquid-radiochromatography. The yield of the two biliary metabolites was 5% of the administered tritium after three hours and the yield of fecal N-acetyl-omega-carboxy-leukotriene E4 was 3.5% after three days.
Orning
L
L
Department of Physiological Chemistry, Karolinska Institutet, Stockholm, Sweden.
Keppler
A
A
Midtvedt
T
T
Hammarström
S
S
D000818:Animals; D001646:Bile / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005243:Feces / Q000032:analysis; D005856:Germ-Free Life; D017999:Leukotriene E4 / Q000031:analogs & derivatives*; D008297:Male; D010084:Oxidation-Reduction; D051381:Rats; D011919:Rats, Inbred Strains; D013189:SRS-A / Q000031:analogs & derivatives / Q000096:biosynthesis / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet
D013189:SRS-A; C059593:20-hydroxy-N-acetylleukotriene E4; C054177:20-carboxy-N-acetylleukotriene E4; D017999:Leukotriene E4