Effect of DTPA to antibody ratio on chemical, immunological and biological properties of the 111In-labelled F(ab')2 fragment of the monoclonal antibody 431/31.
When used for immunoscintigraphic detection of malignant tumours, monoclonal antibodies are frequently labelled with indium-111. This study investigated whether antibody properties are affected by DTPA, the chelating agent with which the antibody has to be bound before labelling with indium-111. Chromatography, cell binding assays and animal studies indicated marked changes in the chemical, immunological and biological properties of the F(ab')2 antibody fragment 431/31 at DTPA:F(ab')2 ratios greater than 1. It is concluded that, in order to avoid lasting effects on the normal biokinetic behaviour of this antibody, the DTPA:F(ab')2 ratio for the antibody should not exceed 1.
Kuhlmann
L
L
Hoechst Aktiengesellschaft, Frankfurt/Main, F.R.G.
Steinsträsser
A
A
D000911:Antibodies, Monoclonal; D007140:Immunoglobulin Fab Fragments; D007128:Immunoglobulin Fragments; D007205:Indium Radioisotopes; D007553:Isotope Labeling / Q000379:methods*; D004369:Pentetic Acid
D000911:Antibodies, Monoclonal; D007140:Immunoglobulin Fab Fragments; D007128:Immunoglobulin Fragments; D007205:Indium Radioisotopes; D004369:Pentetic Acid