title: Determination of digoxin in serum by on-line immunoadsorptive clean-up high-performance liquid chromatographic separation and fluorescence-reaction detection.
abstract: The use of valve-switching with an anti-digoxin immunoadsorptive clean-up cartridge prior to reversed-phase elution of serum is described. Eluted digoxin is detected by reaction of the unsplit eluent stream with hydrochloric acid in a PTFE coil, followed by fluorescence detection. Routes for optimization and evaluation of the immunoadsorptive clean-up are reported, and the effects on the recovery rate examined. The detection limit of 300 pg/ml and reproducibility of less than 7% for the application of the analytical procedure to serum are reasonably good.
authors.affiliation: Boehringer-Mannheim GmbH, Tutzing, F.R.G.
authors.forename: E
authors.initials: E
authors.lastname: Reh
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004077:Digoxin / Q000097:blood*; D006801:Humans; D007164:Immunosorbents; D013050:Spectrometry, Fluorescence
chemical_list: D007164:Immunosorbents; D004077:Digoxin