title: [Isolation and identification of the enzyme complex in Streptomyces recifensis var. lyticus 2435 with beta-lactamase activity].
abstract: Ability of an enzyme complex from Streptomyces recifensis var. lyticus 2435 to inactivate beta-lactam antibiotics was shown. Two lytic endopeptidases with beta-lactamase activity were isolated and identified as beta-lactamases of classes II and V according to the Richmond and Sykes classification system. The ability of the endopeptidases to hydrolyze the beta-lactam ring confirmed the absence of strict substrate specificity in them. Correlation between the capacity of the lytic endopeptidases for lysing staphylococcal cells and their capacity for inactivating beta-lactam antibiotics was observed.
authors.forename: L E
authors.initials: LE
authors.lastname: Gnilomedova
authors.forename: N V
authors.initials: NV
authors.lastname: Kukushkina
authors.forename: Iu S
authors.initials: IuS
authors.lastname: Babenko
mesh_terms: D000900:Anti-Bacterial Agents / Q000037:antagonists & inhibitors; D001433:Bacteriolysis / Q000187:drug effects; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004352:Drug Resistance, Microbial; D006868:Hydrolysis; D013302:Streptomyces / Q000201:enzymology*; D001618:beta-Lactamases / Q000032:analysis / Q000302:isolation & purification* / Q000494:pharmacology; D047090:beta-Lactams
chemical_list: D000900:Anti-Bacterial Agents; D047090:beta-Lactams; D001618:beta-Lactamases
vernacular_title: Vydelenie i identifikatsiia fermentov kompleksa Streptomyces recifensis var. lyticus 2435, obladaiushchikh beta-laktamaznoĭ aktivnost'iu.