New aspects in the study of the mechanism of intestinal absorption of thiamine in rats.
Intestinal absorption of thiamine was studied in rats using such metabolic inhibitors as ouabain, sodium azide and theophylline. These substances changed certain biochemical parameters of the mucosa in the small intestine affecting the level of thiamine transport across the intestinal epithelium. It was found that the intestinal transport of thiamine at lower concentration was an active process depending on the activity of Na, K, Mg-ATPase in the intestinal microvilli, and on the activity of mitochondrial processes. The used metabolic inhibitors increased the intestinal diffusion of thiamine at its higher concentration suggesting that these inhibitors changed membrane permeability by affecting enterocyte homeostasis. At both studied concentrations thiamine was rapidly absorbed from the digestive tract reaching the state of saturation which suggested a carrier character of this transport.
Szymański
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Military Institute of Hygiene and Epidemiology, Warsaw.
D000818:Animals; D001692:Biological Transport; D005260:Female; D007408:Intestinal Absorption; D007421:Intestine, Small / Q000378:metabolism*; D008928:Mitochondria / Q000378:metabolism; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains; D012996:Solutions; D013831:Thiamine / Q000008:administration & dosage / Q000493:pharmacokinetics*
D012996:Solutions; D013831:Thiamine