The metabolism of aminophenols in erythrocytes.
1. Like 2- and 4-dimethylaminophenol, 4-aminophenol in the presence of oxyhaemoglobin forms numerous adducts with glutathione (GSH). Using 14C-4-aminophenol and 3H-glutathione, ten different thioethers were isolated, by h.p.l.c., with isotope ratios of 1:1, 1:2, 1:3, respectively. The structural identification of the different thioethers is under current investigation. 2. In erythrocytes of human and dog, and in dog blood, in vivo, the same pattern of 4-aminophenol conjugates with GSH was found. 3. In vivo, 5% of administered 4-aminophenol is converted into thioethers within erythrocytes, accompanied by a 60% decrease in the cellular GSH, indicating the role of erythrocytes in the biotransformation of xenobiotics.
Eckert
K G
KG
Walther-Straub-Institut für Pharmakologie und Toxikologie, Ludwig-Maximilians-Universität München, F.R. Germany.
D000627:Aminophenols / Q000097:blood*; D000818:Animals; D001711:Biotransformation; D004285:Dogs; D004912:Erythrocytes / Q000378:metabolism*; D005978:Glutathione / Q000097:blood; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D008706:Methemoglobin / Q000378:metabolism; D013440:Sulfides / Q000097:blood
D000627:Aminophenols; D013440:Sulfides; D008706:Methemoglobin; D005978:Glutathione; C026729:4-aminophenol