Rapid isolation of thymosin beta 4 from thymosin fraction 5 by preparative high-performance liquid chromatography.
We have developed a rapid, efficient, and reproducible two-step method for the purification of thymosin beta 4 (T beta 4) from thymosin fraction 5 (TF5). This purification is based on the use of high-performance preparative/semi-preparative and analytical reversed-phase (Delta-Pak C18) chromatographic columns. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and amino acid compositional analysis have shown that natural, purified T beta 4 is identical to synthetic T beta 4. This procedure can be used to isolate other biologically active peptides from TF5 in sufficient quantity for characterization.
Badamchian
M
M
Department of Biochemistry, George Washington University, School of Medicine, Washington, DC 20037.
Strickler
M P
MP
Stone
M J
MJ
Goldstein
A L
AL
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D011863:Radioimmunoassay; D013056:Spectrophotometry, Ultraviolet; D013947:Thymosin / Q000031:analogs & derivatives* / Q000032:analysis / Q000302:isolation & purification; D000099201:Thymosin beta(4)
D000596:Amino Acids; D013947:Thymosin; D000099201:Thymosin beta(4); C033782:thymosin fraction 5