title: A fern spore storage protein is genetically similar to the 1.7 S seed storage protein of Brassica napus.
abstract: The ostrich fern, Matteuccia struthiopteris L., contains two globulin spore storage proteins of 2.2 S and 11.3 S, with physical characteristics similar to those of seed storage proteins of Brassica napus (rapeseed) and Raphanus sativus (radish). By the use of a cloned cDNA that encodes the 1.7 S B. napus storage protein (napin), gene sequences that hybridized with napin were detected in fern nuclear DNA, and a 900-nucleotide homologous mRNA was detected in developing spores. In vitro translation of this fern mRNA produced a 22-kD polypeptide comparable in size to the 21-kD precursor polypeptide identified in Brassica. No hybridizations were observed between the Brassica 12 S clone and either fern DNA or developing spore mRNA.
authors.affiliation: Department of Biological Sciences, Dartmouth College, Hanover, New Hampshire 03755.
authors.forename: T S
authors.initials: TS
authors.lastname: Templeman
authors.forename: D B
authors.initials: DB
authors.lastname: Stein
authors.forename: A E
authors.initials: AE
authors.lastname: DeMaggio
mesh_terms: D015139:Blotting, Southern; D001937:Brassica / Q000235:genetics*; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D005796:Genes; D009693:Nucleic Acid Hybridization; D010940:Plant Proteins / Q000235:genetics*; D010944:Plants / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D013170:Spores
chemical_list: D010940:Plant Proteins; D012333:RNA, Messenger; D004247:DNA