title: Prolactin upstream factor I mediates cell-specific transcription.
abstract: DNA sequence-specific chromatography was used to purify prolactin upstream factor I (PUF-I) approximately 10,000- to 20,000-fold from rat GH3 cells. The purified transcription factor reconstituted enhanced pituitary-specific prolactin RNA synthesis in nonpituitary in vitro transcription assays. In vitro mutagenesis demonstrated that the capacity to stimulate prolactin gene transcription was directly correlated with PUF-I binding to an A+T-rich region located from -63 to -36 in the prolactin 5'-flanking DNA. We propose that PUF-I is a critical modulator of transcriptional activity in pituitary cells and has a central role in the stimulation of prolactin gene transcription in the mammalian pituitary lactotroph.
authors.affiliation: Department of Cellular and Structural Biology, University of Texas Health Science Center, San Antonio 78284.
authors.forename: Z D
authors.initials: ZD
authors.lastname: Cao
authors.forename: E A
authors.initials: EA
authors.lastname: Barron
authors.forename: Z D
authors.initials: ZD
authors.lastname: Sharp
mesh_terms: D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D005786:Gene Expression Regulation; D005796:Genes; D008969:Molecular Sequence Data; D011388:Prolactin / Q000235:genetics*; D011485:Protein Binding; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D014157:Transcription Factors; D011388:Prolactin