Horizontal two-dimensional electrophoresis with immobilized pH gradients using PhastSystem.
Protocols for horizontal two-dimensional electrophoresis with immobilized pH gradients in the first dimension were modified for horizontal micro two-dimensional electrophoresis using PhastSystem. Different equilibration conditions of the first-dimensional immobilized pH gradient gel strip prior to second-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis were evaluated. Silver stained two-dimensional patterns were obtained within 3.5 h.
Görg
A
A
Lehrstuhl für Allgemeine Lebensmitteltechnologie, Technische Universität München, Freising-Weihenstephan, Federal Republic of Germany.
Postel
W
W
Günther
S
S
Friedrich
C
C
D015180:Electrophoresis, Gel, Two-Dimensional / Q000295:instrumentation / Q000379:methods*; D006863:Hydrogen-Ion Concentration