[Electrogenic character of the functioning of H+-ATPase of synaptic vesicles in the rat brain].
Transmembrane potential (TMP) of synaptic vesicles (SV) from the rat brain was registered by means of potential-sensitive fluorescent probe 3,3' dipropylthiocarbocyanine iodide (dis-C3-(5)). It was found that Mg-ATP generates TMP on the SV membrane suspended in sucrose-buffer medium. TMP did not appear if 0.25 M sucrose medium was replaced by 0.125 M KCl medium or 0.125 NaCl medium, or if the SV membrane was disrupted by triton X-100 or alamethicin, or if there was protonophore m-chlorophenylhydrazone or N-ethylmaleimide influence on SV. The data obtained indicate that functioning of SV proton pump is electrogenic and the level of TMP depends on the presence of anion Cl- in the incubation medium.
Antonikov
I M
IM
Glebov
R N
RN
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D052160:Benzothiazoles; D001921:Brain / Q000502:physiology*; D002232:Carbocyanines; D005456:Fluorescent Dyes; D066298:In Vitro Techniques; D008564:Membrane Potentials / Q000187:drug effects; D051381:Rats; D013572:Synaptic Vesicles / Q000502:physiology*
D052160:Benzothiazoles; D002232:Carbocyanines; D005456:Fluorescent Dyes; C012944:3,3'-dipropyl-2,2'-thiadicarbocyanine; D000255:Adenosine Triphosphate
Elektrogennyĭ kharakter funktsionirovaniia H+-ATFazy sinapticheskikh puzyr'kov mozga krys.