title: Fast protein liquid chromatography of botulinum neurotoxin types A, B and E.
abstract: Three antigenically different botulinum neurotoxins (NTs, relative molecular mass approximately 150,000), classically distinguished only by specific antisera, were for the first time chromatographically resolved. Mixed NTs eluted from a Mono-Q column in order of types E, A and B, and from Mono-S as B, E and A. Type A and B NTs were successfully chromatographed on the cation-exchange Mono-S column above their isoelectric points. Purification of type A and B NTs by automated liquid chromatography was also accomplished for the first time. Type A, B and E NTs were purified by application on an anion-exchange Mono-Q column, followed by use of a cation-exchange Mono-S column.
authors.affiliation: Food Research Institute, University of Wisconsin, Madison 53706.
authors.forename: M A
authors.initials: MA
authors.lastname: Woody
authors.forename: B R
authors.initials: BR
authors.lastname: DasGupta
mesh_terms: D001905:Botulinum Toxins / Q000032:analysis*; D002021:Buffers; D002850:Chromatography, Gel; D002853:Chromatography, Liquid; D004591:Electrophoresis, Polyacrylamide Gel
chemical_list: D002021:Buffers; D001905:Botulinum Toxins