title: A rapid assay for measuring both colony size and cytolytic activity of limiting dilution microcultures.
abstract: A combined 51Cr-release/MTT dye method is described for accurately measuring cytolytic activity and colony size in the same set of culture microwells. The method was applied to the study of cell-mediated lympholysis (CML) in limiting dilution analysis (LDA) cultures of human PBL from a renal transplant recipient and a healthy control. The results showed that the combined CML/MTT method could detect differences in lytic activity per cell in LDA cultures, and thus is a useful adjunct to standard precursor frequency analysis.
authors.affiliation: Department of Surgery, University of Wisconsin, Madison 53792.
authors.forename: A
authors.initials: A
authors.lastname: Grailer
authors.forename: H W
authors.initials: HW
authors.lastname: Sollinger
authors.forename: W J
authors.initials: WJ
authors.lastname: Burlingham
mesh_terms: D002478:Cells, Cultured; D003601:Cytotoxicity Tests, Immunologic; D006801:Humans; D066298:In Vitro Techniques; D013602:T-Lymphocytes, Cytotoxic / Q000032:analysis*