Inhibitory effects of prostaglandin E1 on T-cell mediated cytotoxicity against isolated mouse liver cells.
The effects of prostaglandin E1 on cell-mediated cytotoxicity against hepatocytes were investigated using an in vitro cytotoxic assay system. Isolated liver cells from normal C57BL/6 mice were used as the target cells, and effector cells were obtained from spleens of C57BL/6 mice in which experimental hepatitis had been induced by immunization with syngeneic liver antigens. In this assay system, spleen T cells adhering to nylon wool demonstrated a high cytotoxic activity against target liver cells. The cytotoxicity was markedly reduced by prostaglandin E1 at concentrations greater than 10(-7) M. Maximum suppressive activity was obtained when prostaglandin E1 was continuously present during the assay period. By contrast, indomethacin, a specific inhibitor of prostaglandin synthesis, enhanced the cytotoxic activity of effector cells. These data seem to indicate that exogenously added prostaglandin E1 has an inhibitory effect on cell-mediated cytotoxicity of effector spleen cells against target hepatocytes.
Ogawa
M
M
First Department of Internal Medicine, Chiba University School of Medicine, Japan.
Mori
T
T
Mori
Y
Y
Ueda
S
S
Yoshida
H
H
Kato
I
I
Iesato
K
K
Wakashin
Y
Y
Wakashin
M
M
Okuda
K
K
D000527:Alprostadil / Q000494:pharmacology* / Q000502:physiology; D000818:Animals; D002478:Cells, Cultured; D003602:Cytotoxicity, Immunologic / Q000187:drug effects*; D006520:Hepatitis, Animal / Q000276:immunology; D007213:Indomethacin / Q000494:pharmacology; D008099:Liver / Q000276:immunology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D000527:Alprostadil; D007213:Indomethacin