Extraluminally applied acetylcholine and oxyhemoglobin on the release and action of EDRF.
Acetylcholine applied intra- and extraluminally to the donor tissue (dog femoral artery segment with endothelium) produced a relaxation of the bioassay tissue (coronary artery strip without endothelium). Extraluminal acetylcholine was 1/44 as potent as intraluminal acetylcholine (10(-7) M). Extraluminal treatment with oxyhemoglobin halved the relaxation caused by acetylcholine applied intraluminally. Acetylcholine and oxyhemoglobin applied extraluminally appear to be translocated to the endothelium, resulting in vasodilatation via the release of endothelium-derived relaxing factor (EDRF) and attenuation of EDRF actions, respectively.
Toda
N
N
Department of Pharmacology, Shiga University of Medical Sciences, Ohtsu, Japan.
Minami
Y
Y
Onoue
H
H
D000109:Acetylcholine / Q000494:pharmacology*; D000818:Animals; D001688:Biological Products / Q000378:metabolism / Q000494:pharmacology*; D001794:Blood Pressure / Q000187:drug effects; D004285:Dogs; D005260:Female; D005263:Femoral Artery / Q000187:drug effects; D066298:In Vitro Techniques; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000378:metabolism*; D009569:Nitric Oxide; D010108:Oxyhemoglobins / Q000494:pharmacology*; D014665:Vasodilator Agents / Q000378:metabolism / Q000494:pharmacology*
D001688:Biological Products; D010108:Oxyhemoglobins; D014665:Vasodilator Agents; D009569:Nitric Oxide; D000109:Acetylcholine