Immobilized pH gradient isoelectric focusing of cerebrospinal fluid proteins.
Isoelectric focusing in immobilized pH gradients, supplemented with 0.5% w/v carrier ampholytes was applied for studies of native proteins, especially immunoglobulin G, in cerebrospinal fluid and serum. All 72 paired samples were run on pH 4-10 gels; 25 of them were also examined in pH 7-10 gels. Silver staining and nitrocellulose blotting with amplified immunoperoxidase detection of immunoglobulin G were used for protein visualization. Intrathecally produced immunoglobulin G was resolved into sharply focused, straight and easily identifiable fractions. The pH gradients were stable and the inter-gel reproducibilities of individual immunoglobulin G patterns were good.
Pirttilä
T
T
Department of Neurology, University of Tampere, Sweden.
Frey
H
H
Mattila
K
K
Sidén
A
A
D000328:Adult; D000368:Aged; D002556:Cerebrospinal Fluid Proteins / Q000302:isolation & purification*; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007074:Immunoglobulin G / Q000134:cerebrospinal fluid / Q000302:isolation & purification; D007525:Isoelectric Focusing / Q000379:methods; D008297:Male; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000134:cerebrospinal fluid; D009422:Nervous System Diseases / Q000134:cerebrospinal fluid; D012016:Reference Values; D012709:Serum Albumin / Q000134:cerebrospinal fluid / Q000302:isolation & purification
D002556:Cerebrospinal Fluid Proteins; D007074:Immunoglobulin G; D012709:Serum Albumin