Solubilization of rat kidney plasma membrane proteins associated with 3H-aldosterone.
The treatment of rat kidney plasma membranes with sodium dodecyl sulphate (SDS) did not essentially affect the ability of the membranes for 3H-aldosterone binding as compared with the intact plasma membranes (Ozegović et al., 1977). A gel filtration of 3H-aldosterone-kidney plasma membranes complex on Sepharose 6B yielded 2 protein and 2 3H-aldosterone peaks. The proteins which were eluted in the first peak were associated with the first 3H-aldosterone peak while the second 3H-aldosterone peak was eluted with Ve corresponding to Ve of free 3H-aldosterone. Spironolactone, a competitive antagonist of aldosterone, prevented the binding of 3H-aldosterone to the membrane proteins. The results demonstrated a high affinity of the kidney plasma membranes solubilized with SDS and a specificity of aldosterone binding to the plasma membrane proteins of higher molecular mass.
Ozegović
B
B
Laboratory of Pharmacology, Faculty of Pharmacy, University of Zagreb, Yugoslavia.
Dobrović-Jenik
D
D
Milković
S
S
D000315:Adrenalectomy; D000450:Aldosterone / Q000378:metabolism; D000818:Animals; D001667:Binding, Competitive / Q000187:drug effects*; D002850:Chromatography, Gel; D007668:Kidney / Q000166:cytology*; D007700:Kinetics; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012967:Sodium Dodecyl Sulfate; D012995:Solubility; D013148:Spironolactone / Q000494:pharmacology; D014316:Tritium
D008565:Membrane Proteins; D014316:Tritium; D013148:Spironolactone; D012967:Sodium Dodecyl Sulfate; D000450:Aldosterone