A new chromogenic substrate for assay and detection of alpha-amylase.
A new soluble chromogenic substrate for alpha-amylase was prepared by coupling partially hydrolyzed starch with a dye, Ostazin brilliant red H-3B. The substrate is precipitable from buffered solutions with ethanol and is equally suitable for assay of alpha-amylase, detection of separated alpha-amylase isoenzymes in gels, and selection of microbial producers of the enzyme.
Biely
P
P
Institute of Chemistry, Slovak Academy of Sciences, Bratislava, Czechoslovakia.
Mislovicová
D
D
Markovic
O
O
Kalác
V
V
D002863:Chromogenic Compounds; D004396:Coloring Agents; D007525:Isoelectric Focusing; D013213:Starch; D014227:Triazines; D000516:alpha-Amylases / Q000032:analysis*
D002863:Chromogenic Compounds; D004396:Coloring Agents; D014227:Triazines; C011535:procion brilliant red H 3B; D013213:Starch; D000516:alpha-Amylases