title: Inhibitory effect of transforming growth factor-beta on epidermal growth factor-induced proliferation of cultured rat aortic smooth muscle cells.
abstract: This study was conducted to investigate the effect of transforming growth factor-beta (TGF-beta) on the proliferation of cultured rat aortic smooth muscle cells (SMCs). DNA synthesis, measured by the incorporation of [3H] thymidine, and the cell number of monolayered SMCs were measured after incubation with TGF-beta (1-100 ng/ml) in the presence or absence of epidermal growth factor (EGF; 100 ng/ml). TGF-beta alone did not affect DNA synthesis of SMCs. EGF significantly increased both DNA synthesis and cell number, while TGF-beta inhibited the increase in both in a dose-dependent manner without accompanying the significant cellular damage. These results indicate that TGF-beta exerts an inhibitory effect on the proliferation of cultured SMCs provoked by EGF.
authors.affiliation: Department of Geriatrics, Faculty of Medicine, University of Tokyo, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Ouchi
authors.forename: J
authors.initials: J
authors.lastname: Hirosumi
authors.forename: M
authors.initials: M
authors.lastname: Watanabe
authors.forename: A
authors.initials: A
authors.lastname: Hattori
authors.forename: T
authors.initials: T
authors.lastname: Nakamura
authors.forename: H
authors.initials: H
authors.lastname: Orimo
mesh_terms: D000818:Animals; D002455:Cell Division / Q000187:drug effects*; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D004815:Epidermal Growth Factor / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D004987:Ethers / Q000494:pharmacology; D015759:Ionomycin; D009131:Muscle, Smooth, Vascular / Q000166:cytology*; D017830:Octoxynol; D011092:Polyethylene Glycols / Q000494:pharmacology; D051381:Rats; D013997:Time Factors; D015291:Transforming Growth Factors / Q000494:pharmacology*
chemical_list: D004987:Ethers; D011092:Polyethylene Glycols; D015759:Ionomycin; D004815:Epidermal Growth Factor; D015291:Transforming Growth Factors; D017830:Octoxynol; D004247:DNA