HLA DPw1 class II molecules on human T cells.
Human T cells express HLA class II antigens when activated by mitogens, alloantigens, or nominal antigens such as influenza virus. However, little is known about why they are expressed and the extent to which subsets of class II molecules (DR, DQ, and DP) are expressed. From studies with allocytotoxic antisera it is clear that DR and DQ molecules are expressed by T cells; cell surface expression of DP is more ambiguous because cellular typing methodologies are required. Alloreactive T-cell clones, specific for DPw1-associated antigens, were derived by limiting dilution in the presence of DPw1-positive stimulator PBLs and IL-2 and screened on panels of DPw1-positive and DPw1-negative PBL's in proliferation assays. Clones that recognized DPw1-associated determinants were then assayed for responses using as stimulators, irradiated T-cell clones derived from a DPw1-positive donor. Of seven DPw1-specific clones, one (TLC 56.26) was highly responsive to the alloantigens expressed on the stimulator T-cell panel. Six such clones gave a range of lower responses to T cells although capable of recognizing DP-associated determinants on PBL stimulators.
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Immunogenetics Research Section, Blood Center of Southeastern Wisconsin, Milwaukee 53233.
D000911:Antibodies, Monoclonal / Q000276:immunology; D001667:Binding, Competitive; D002999:Clone Cells / Q000276:immunology; D006681:HLA-D Antigens; D006682:HLA-DP Antigens; D006801:Humans; D008213:Lymphocyte Activation; D013601:T-Lymphocytes / Q000276:immunology*
D000911:Antibodies, Monoclonal; D006681:HLA-D Antigens; D006682:HLA-DP Antigens