title: Analysis of TSH receptors and microsomal antigen in different human thyroid tissue specimens.
abstract: Affinity labelling with a 125I-labelled photoactive derivative of TSH (HSAB-TSH) was used to analyse TSH receptor size in the following specimens of human thyroid tissue: (1) cold nodules; (2) autonomous nodules; (3) papillary carcinoma; (4) medullary carcinoma; (5) metastasis of papillary carcinoma to lymph node; (6) anaplastic carcinoma, and (7) Graves' thyroid. In addition, a sample of histologically normal thyroid tissue surrounding specimens 1-4 was analysed in each case. Thyroid microsomes were also prepared from the tissue samples, solubilized using 1% deoxycholate and labelled with 125I. The preparations were immunoprecipitated using microsomal autoantibodies and protein A and analysed by SDS-PAGE and autoradiography. These studies indicated that no differences in the characteristics of the TSH receptor or of microsomal antigen were observed in the tissue samples 1-3 and 7. Neither protein was detected in tissue specimens 4-6.
authors.affiliation: Endocrine Immunology Unit, University of Wales College of Medicine, Heath Park, Cardiff, UK.
authors.forename: J
authors.initials: J
authors.lastname: Furmaniak
authors.forename: S
authors.initials: S
authors.lastname: Davenport
authors.forename: Y
authors.initials: Y
authors.lastname: Nakajima
authors.forename: U
authors.initials: U
authors.lastname: Kaiser
authors.forename: H W
authors.initials: HW
authors.lastname: Muller-Gartner
authors.forename: F W
authors.initials: FW
authors.lastname: Buckmann
authors.forename: C
authors.initials: C
authors.lastname: Pegg
authors.forename: B
authors.initials: B
authors.lastname: Rees Smith
mesh_terms: D001324:Autoantigens / Q000032:analysis*; D001345:Autoradiography; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008861:Microsomes / Q000276:immunology*; D011989:Receptors, Thyrotropin / Q000032:analysis*; D013959:Thyroid Diseases / Q000276:immunology*; D013961:Thyroid Gland / Q000276:immunology*
chemical_list: D001324:Autoantigens; D011989:Receptors, Thyrotropin