A simple enzyme-linked immunosorbent assay for sex hormone-binding globulin.
A simple enzyme-linked immunosorbent assay (ELISA) for sex hormone-binding globulin (SHBG) has been developed. Polyclonal antibody raised to SHBG purified to homogeneity was employed. The ELISA, which may be performed in under 4 h, shows no cross-reactivity with other serum proteins, has a sensitivity of less than 1.2 fmol per sample, demonstrates excellent correlation with ligand-binding techniques (r = 0.996; p less than 0.0001), and has intra- and inter-assay coefficients of variation of between 5-9% and 7-11% respectively.
Iqbal
M J
MJ
Tumour Biology Unit, King's College School of Medicine and Dentistry, London, UK.
Forbes
A
A
Valyani
S H
SH
Corbishley
T P
TP
Wilkinson
M L
ML
D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D008024:Ligands; D011817:Rabbits; D015203:Reproducibility of Results; D012738:Sex Hormone-Binding Globulin / Q000032:analysis*
D008024:Ligands; D012738:Sex Hormone-Binding Globulin