title: Pasteur yeasts system, a test-kit for yeasts identification. Its evaluation in comparison with three commercial methods and conventional procedures.
abstract: The Pasteur Yeasts System is a commercial prepared kit and scheme for the rapid (48 h) identification of 23 yeasts belonging to 7 genera. The method consists of two parts: a gallery of media to evaluate germ tube production, urease activity, tetrazolium reduction, cycloheximide sensibility and fermentation of three carbohydrates, and an auxanographic method to determine the assimilation pattern of 16 carbohydrates. This system was tested in comparison with API 20 C Auxanogram, Mycotube, Candida Check and conventional procedures to identify 40 yeast strains. Pasteur Yeasts System provided correct identifications for 97.5% of the organisms and proved to have a high reproducibility, accuracy and reliability. The method is useful and facilitates the identification of pathogenic yeasts from clinical specimens in the routine laboratory.
authors.affiliation: Institute of Medical Microbiology, University of Milan, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Riviera
authors.forename: M G
authors.initials: MG
authors.lastname: Bellotti
mesh_terms: D005069:Evaluation Studies as Topic; D011237:Predictive Value of Tests; D011933:Reagent Kits, Diagnostic; D015003:Yeasts / Q000302:isolation & purification*
chemical_list: D011933:Reagent Kits, Diagnostic