Transfected plasmacytoma cells do not transport the membrane form of IgM to the cell surface.
Expression vectors coding for membrane-bound IgM antibodies were introduced into myeloma and B lymphoma cells. Only the lymphoma but not the myeloma cells were able to express the antibodies on the cell surface, although in both cases, complete antibodies were assembled intracellularly. In myeloma cells, the Ig molecules did not reach the Golgi compartment. Thus, the intracellular transport of membrane-bound antibodies is controlled in the B cell lineages in a developmentally ordered fashion.
Hombach
J
J
Institute for Genetics, University of Cologne, Federal Republic of Germany.
Sablitzky
F
F
Rajewsky
K
K
Reth
M
M
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology / Q000378:metabolism; D001692:Biological Transport; D002460:Cell Line; D007075:Immunoglobulin M / Q000378:metabolism*; D007424:Intracellular Fluid / Q000276:immunology / Q000378:metabolism; D051379:Mice; D010954:Plasmacytoma / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D011947:Receptors, Antigen, B-Cell / Q000378:metabolism*; D014162:Transfection
D007075:Immunoglobulin M; D011947:Receptors, Antigen, B-Cell