title: Chromosomal gene transfer in Spiroplasma citri.
abstract: The study of resistance marker rearrangement in Spiroplasma citri mutants provides evidence of transfer of chromosomal information followed by recombination. This is the first report of such a transfer in vivo in the mollicutes--that is, in the smallest self-replicating organisms. The double-resistant phenotypes obtained are stable even without selection pressure. The mechanism of gene transfer is insensitive to deoxyribonuclease, requires contact, and possibly, areas of fusion of the cell membranes; it shares properties with the transfer by protoplast fusion in Gram-positive bacteria. The extensive degenerative evolution of mollicutes has retained, in S. citri, bacterial functions of chromosomal transfer and recombination.
authors.affiliation: Laboratoire de Génétique Moléculaire, Université de Bordeaux II-INRA, C.R.A. de Bordeaux, Pont-de-la-Maye, France.
authors.forename: G
authors.initials: G
authors.lastname: Barroso
authors.forename: J
authors.initials: J
authors.lastname: Labarère
mesh_terms: D001149:Arsenates / Q000494:pharmacology; D002876:Chromosomes, Bacterial; D003001:Cloning, Molecular; D003470:Culture Media; D004352:Drug Resistance, Microbial / Q000235:genetics; D006540:Herbicides / Q000494:pharmacology; D009154:Mutation; D010087:Oxides / Q000494:pharmacology; D010641:Phenotype; D011995:Recombination, Genetic; D013149:Spiroplasma / Q000187:drug effects / Q000235:genetics*; D013997:Time Factors; D014162:Transfection; D014638:Vanadates / Q000494:pharmacology
chemical_list: D001149:Arsenates; D003470:Culture Media; D006540:Herbicides; D010087:Oxides; D014638:Vanadates; C025657:arsenic acid