Spectrofluorometric assay of thiamine hydrochloride in injections and multivitamin preparations.
A spectrofluorometric method is described for the determination of thiamine hydrochloride. The method is based on formation of a fluorescent product by oxidation of thiamine HCl with 2,3,5,6-tetrabromo-1,4-benzoquinone in aqueous acetonitrile solution. The reaction product is stable for at least 6 h and shows excitation and emission maxima around 355 and 420 nm, respectively. The method is highly selective for thiamine HCl in the presence of other B vitamins. Thiamine HCl can be determined at concentrations as low as 20-160 ng/mL of the final solutions. When the method was applied to the determination of thiamine HCl added to some commercially available multivitamin preparations, recoveries were 98.78-99.98%.
Mohamed
A M
AM
Assiut University, Faculty of Pharmacy, Department of Pharmaceutical Chemistry, Egypt.
Hussein
S A
SA
el-Shabouri
S R
SR
D002214:Capsules; D012996:Solutions; D012997:Solvents; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013607:Tablets; D013831:Thiamine / Q000032:analysis*; D014815:Vitamins / Q000032:analysis*
D002214:Capsules; D012996:Solutions; D012997:Solvents; D013607:Tablets; D014815:Vitamins; D013831:Thiamine