Regulation of coenzyme A transferase and acetoacetate decarboxylase activities in Clostridium acetobutylicum.
The activity of two enzymes involved in acetone production in Clostridium acetobutylicum, acetoacetate decarboxylase and coenzyme A transferase, was studied under acidogenic or solventogenic conditions. Acetoacetate decarboxylase activity was low under acidogenic conditions and after pyruvate addition. Under the same conditions, coenzyme A transferase activity was high. A mutant which lacked acetoacetate decarboxylase activity but was positive for coenzyme A transferase activity was isolated.
Janati-Idrissi
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R
Laboratoire de Chimie Biologique I, Université de Nancy 1, Vandoeuvre-lès-Nancy, France.
Junelles
A M
AM
Petitdemange
H
H
Gay
R
R
D000096:Acetone / Q000378:metabolism; D002262:Carboxy-Lyases / Q000378:metabolism*; D003013:Clostridium / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D015256:Coenzyme A-Transferases / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D009154:Mutation; D011773:Pyruvates / Q000494:pharmacology; D019289:Pyruvic Acid
D011773:Pyruvates; D000096:Acetone; D019289:Pyruvic Acid; D015256:Coenzyme A-Transferases; D002262:Carboxy-Lyases; C023743:acetoacetate decarboxylase