title: Hypoxanthine, xanthine, urate and creatinine concentration gradients in cerebrospinal fluid.
abstract: The purine metabolites hypoxanthine, xanthine and urate as well as creatinine were measured in cerebrospinal fluid (CSF) from two groups of patients and a reference sample group. In one of the patient groups lumbar CSF was collected in 2 ml portions until a total volume of 14 ml was withdrawn. Every second portion was analysed for its content of the metabolites in focus. In the other patient group both cisternal CSF and a fixed volume (20 ml) of lumbar CSF were obtained and analysed. An increase in concentration of hypoxanthine, xanthine and creatinine and a decrease in urate concentration was found in the successive CSF specimens. The mean individual increase in hypoxanthine concentration between the first and the last 2 ml portion was as high as 39.6%, while it was lower for xanthine, 21.5%, and creatinine, 6.7%. The decrease in urate concentration was 17.2%. The results from the other patient group were in good agreement with these findings. The concentrations in the cisternal CSF was 162% of that in lumbar CSF for hypoxanthine, 155% for xanthine, 123% for creatinine and 80% for urate. Mechanisms behind inter- and intraindividual differences in gradients are discussed.
authors.affiliation: Department of Clinical Chemistry, University Hospital, Uppsala, Sweden.
authors.forename: F
authors.initials: F
authors.lastname: Niklasson
authors.forename: J
authors.initials: J
authors.lastname: Hetta
authors.forename: I
authors.initials: I
authors.lastname: Degrell
mesh_terms: D001693:Biological Transport, Active; D002946:Cisterna Magna; D003404:Creatinine / Q000134:cerebrospinal fluid*; D003704:Dementia / Q000134:cerebrospinal fluid; D003863:Depression / Q000134:cerebrospinal fluid; D006801:Humans; D019271:Hypoxanthine; D007042:Hypoxanthines / Q000134:cerebrospinal fluid*; D008161:Lumbosacral Region; D013115:Spinal Canal; D014527:Uric Acid / Q000134:cerebrospinal fluid*; D019820:Xanthine; D014970:Xanthines / Q000134:cerebrospinal fluid*
chemical_list: D007042:Hypoxanthines; D014970:Xanthines; D019820:Xanthine; D014527:Uric Acid; D019271:Hypoxanthine; D003404:Creatinine