Characterization and functional properties of a novel monoclonal antibody which identifies a T cell receptor in chickens.
A monoclonal antibody, mAb6, was produced that specifically bound to chicken T lymphocytes. Immunofluorescence analysis using a fluorescence-activated cell sorter revealed that the antibody reacted with approximately 50% of blood lymphocytes and with approximately 40% of splenocytes and thymocytes. It did not react with bursal cells and erythrocytes. Among different types of hemopoietic cell lines, only a T cell line was reactive with mAb6. When coupled to Sepharose 4B beads, mAb6 was found to be highly mitogenic for chicken T cells. In soluble form, mAb6 inhibited concanavalin A-induced T cell proliferation and the cytotoxic activity of allosensitized T cells, the inhibition occurring in a dose-dependent manner for both assays. Thus, the tissue distribution and the effects of this antibody on T cell responses suggest that mAb6 recognizes a T cell receptor present on a large proportion of chicken T cells.
Cihak
J
J
Institute for Animal Physiology, University of Munich, FRG.
Ziegler-Heitbrock
H W
HW
Trainer
H
H
Schranner
I
I
Merkenschlager
M
M
Lösch
U
U
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000937:Antigen-Antibody Reactions; D002469:Cell Separation; D002645:Chickens / Q000276:immunology*; D003208:Concanavalin A / Q000494:pharmacology; D005434:Flow Cytometry; D006412:Hematopoietic Stem Cells / Q000276:immunology; D008213:Lymphocyte Activation; D011948:Receptors, Antigen, T-Cell / Q000276:immunology*; D013601:T-Lymphocytes / Q000145:classification / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014018:Tissue Distribution
D000911:Antibodies, Monoclonal; D011948:Receptors, Antigen, T-Cell; D003208:Concanavalin A