A continuous acetic acid system for polyacrylamide gel electrophoresis of gliadins and other prolamines.
A polyacrylamide gel electrophoresis system buffered by acetic acid alone was developed for electrophoresis of prolamines. When applied to gliadin electrophoresis, the acetic acid system produces more bands than does a conventional aluminum lactate-lactic acid system (using 12% acrylamide gels). The acetic acid system is relatively simple, requiring a single buffer component that is universally available in high purity.
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Soft Wheat Quality Laboratory, USDA-ARS, Wooster, OH 44691.
D000085:Acetates; D002523:Edible Grain / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005903:Gliadin / Q000032:analysis*; D010940:Plant Proteins / Q000032:analysis*; D055315:Prolamins; D011506:Proteins / Q000032:analysis*
D000085:Acetates; D010940:Plant Proteins; D055315:Prolamins; D011506:Proteins; D005903:Gliadin