Inhibition of cellular phosphatidylinositol turnover by psi-tectorigenin.
Psi-tectorigenin, an isoflavonoid, was isolated from a culture filtrate of actinomycetes as an inhibitor of epidermal growth factor-induced phosphatidylinositol turnover in cultured A431 cells. It inhibited phosphatidylinositol turnover with an IC50 of about 1 microgram/ml; thus, its inhibitory activity was 6-times stronger than that of genistein or orobol. When added to cultured A431 cells psi-tectorigenin inhibited phosphatidylinositol turnover without inhibiting epidermal growth factor receptor tyrosine protein kinase. Thus, psi-tectorigenin is a specific inhibitor of phosphatidylinositol turnover and may be a useful tool for the functional analysis of phosphatidylinositol turnover.
Imoto
M
M
Institute of Microbial Chemistry, Tokyo, Japan.
Yamashita
T
T
Sawa
T
T
Kurasawa
S
S
Naganawa
H
H
Takeuchi
T
T
Bao-quan
Z
Z
Umezawa
K
K
D000192:Actinomycetales / Q000378:metabolism*; D002460:Cell Line; D004815:Epidermal Growth Factor / Q000037:antagonists & inhibitors; D066246:ErbB Receptors / Q000378:metabolism; D005419:Flavonoids / Q000494:pharmacology*; D007529:Isoflavones / Q000302:isolation & purification / Q000494:pharmacology*; D010710:Phosphates / Q000378:metabolism; D010716:Phosphatidylinositols / Q000378:metabolism*; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors
D005419:Flavonoids; D007529:Isoflavones; D010710:Phosphates; D010716:Phosphatidylinositols; D004815:Epidermal Growth Factor; C055439:psi-tectorigenin; D066246:ErbB Receptors; D011505:Protein-Tyrosine Kinases