title: Effects of hematopoietic growth factors on in vitro colony formation by human megakaryocyte progenitor cells.
abstract: In order to study the effects of recombinant and purified hematopoietic growth factors on megakaryocyte (MK) progenitor cells (CFU-MK), enriched populations of human CFU-MK were isolated utilizing fluorescence activated cell sorting after labelling of cells with monoclonal antibodies exhibiting specificity to the My10 (HPCA-1) antigen and the major histocompatibility (MHC) class II (HLA-DR) locus. The CFU-MK cloning efficiency (CE) was 1.1 +/- 0.5% for cells expressing both high densities of My10 and low densities of HLA-DR (My10 DR+). This procedure resulted in an 18 fold increase in CE over NALT- cells. The effects of natural or recombinant human hematopoietic growth factors including erythropoietin (Epo), thrombocytopoiesis stimulating factor (TSF), interleukin 1 alpha (IL-1 alpha), granulocyte colony stimulating factor (G-CSF), granulocyte-macrophage colony stimulating factor (GM-CSF), macrophage colony stimulating factor (CSF-1), and interleukin 3 (IL-3) on MK colony formation by My10 DR+ cells were determined utilizing a defined medium assay system. Neither Epo, TSF, CSF-1, IL-1 alpha nor G-CSF alone augmented MK colony formation above baseline (2.5 +/- 0.8 per 5 x 10(3) My10 DR+ cells plated). By contrast, the addition of GM-CSF and IL-3 each increased CFU-MK colony formation with maximal stimulation occurring following the addition of 200 units/ml of IL-3 and 100 units/ml of GM-CSF. At maximal concentration, IL-3 had a greater ability to promote megakaryocyte colony formation than GM-CSF.
authors.affiliation: Department of Medicine, Indiana Elks Cancer Research Center, Indiana University School of Medicine, Indianapolis 46223.
authors.forename: L
authors.initials: L
authors.lastname: Lu
authors.forename: E
authors.initials: E
authors.lastname: Bruno
authors.forename: R A
authors.initials: RA
authors.lastname: Briddell
authors.forename: C D
authors.initials: CD
authors.lastname: Graham
authors.forename: J E
authors.initials: JE
authors.lastname: Brandt
authors.forename: R
authors.initials: R
authors.lastname: Hoffman
mesh_terms: D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D006133:Growth Substances / Q000494:pharmacology*; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000187:drug effects; D006801:Humans; D008533:Megakaryocytes / Q000166:cytology* / Q000187:drug effects; D011994:Recombinant Proteins / Q000494:pharmacology
chemical_list: D006133:Growth Substances; D011994:Recombinant Proteins