Stimulation of DNA synthesis in cultured primary human mesothelial cells by specific growth factors.
Monolayer cultures of human mesothelial cells made quiescent by serum deprivation are induced to undergo one round of DNA synthesis by platelet-derived growth factor (PDGF), epidermal growth factor (EGF), or transforming growth factor type beta 1 (TGF-beta 1). This one-time stimulation is independent of other serum components. The kinetics for induction of DNA synthesis observed for PDGF, EGF, and TGF-beta 1 are all similar to one another, with a peak of DNA synthesis occurring 24-36 h after the addition of the growth factors. Repetitive rounds of DNA synthesis and cell division do not ensue after addition of PDGF, EGF, or TGF-beta 1 alone or in combination; however, in media supplemented with chemically denatured serum, each of these factors is capable of sustaining continuous replication of mesothelial cells. Stimulation of growth by PDGF and TGF-beta 1 is unusual for an epithelial cell type, and indicates that mesothelial cells have growth regulatory properties similar to connective tissue cells.
Gabrielson
E W
EW
Laboratory of Human Carcinogenesis, National Cancer Institute, Bethesda, MD 20892.
Gerwin
B I
BI
Harris
C C
CC
Roberts
A B
AB
Sporn
M B
MB
Lechner
J F
JF
D002478:Cells, Cultured; D004261:DNA Replication / Q000187:drug effects*; D004815:Epidermal Growth Factor / Q000494:pharmacology; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism; D006133:Growth Substances / Q000494:pharmacology*; D006801:Humans; D007700:Kinetics; D010455:Peptides / Q000494:pharmacology; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D013936:Thymidine / Q000378:metabolism; D015291:Transforming Growth Factors; D014316:Tritium
D006133:Growth Substances; D010455:Peptides; D010982:Platelet-Derived Growth Factor; D014316:Tritium; D004815:Epidermal Growth Factor; D015291:Transforming Growth Factors; D013936:Thymidine