Lysis of murine macrophages infected with intracellular pathogens by interleukin 2-activated killer (LAK) cells in vitro.
To determine if murine lymphocytes activated by interleukin 2 (IL-2) were cytotoxic against syngeneic elicited peritoneal macrophages (M phi) infected with intracellular pathogens, splenocytes that had been cultured with IL-2 for 5 or 10 days were studied in vitro. These cells, [lymphokine-activated killer (LAK) cells] showed significantly greater cytotoxicity against M phi infected with Leishmania major or Legionella pneumophila than against uninfected M phi. Preferential cytotoxicity against infected M phi was best shown using effector-to-target-cell ratios of 1:1-20:1; when ratios greater than or equal to 40:1 were employed, uninfected M phi were also lysed. The extent to which M phi that had been incubated with L. major were lysed depended upon the proportion of M phi containing intracellular organisms. After infection with L. major, the duration of incubation did not appear to influence the degree of lysis by LAK cells.
Resnick
M
M
Department of Comparative Medicine, Hadassah Medical School, Jerusalem, Israel.
Roguel
N
N
Bercovier
H
H
Enk
C
C
Frankenburg
S
S
Kedar
E
E
D000818:Animals; D003602:Cytotoxicity, Immunologic; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D007376:Interleukin-2 / Q000494:pharmacology*; D007424:Intracellular Fluid / Q000276:immunology / Q000382:microbiology / Q000469:parasitology; D007694:Killer Cells, Natural / Q000276:immunology*; D007875:Legionella / Q000276:immunology*; D007895:Leishmania tropica / Q000276:immunology*; D008213:Lymphocyte Activation; D008264:Macrophages / Q000276:immunology* / Q000382:microbiology / Q000469:parasitology; D008297:Male; D051379:Mice; D013997:Time Factors
D007376:Interleukin-2