title: Qualitative study of fecal alpha 1-proteinase inhibitor in normal subjects and patients with Crohn's disease.
abstract: We applied sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting to analyze fecal alpha 1-proteinase inhibitor (alpha 1 PI) from healthy subjects and patients with Crohn's disease. A component with Mr 38,000 was characterized in normal fecal extracts as well as in six pathological samples. In these cases, the Crohn's disease activity index (CDAI), a clinical index of severity of the disease, was 170 (SEM 47). In contrast, alpha 1 PI of Mr 51,000 was detected in fecal extracts from eight patients with active Crohn's disease (CDAI = 287, SEM 39). We conclude that fecal alpha 1 PI can be considered a marker of intestinal disease activity.
authors.affiliation: Laboratoire de Biochimie, Faculté de Pharmacie, Lille, France.
authors.forename: C
authors.initials: C
authors.lastname: Mizon
authors.forename: C
authors.initials: C
authors.lastname: Becuwe
authors.forename: M
authors.initials: M
authors.lastname: Balduyck
authors.forename: J F
authors.initials: JF
authors.lastname: Colombel
authors.forename: A
authors.initials: A
authors.lastname: Cortot
authors.forename: J
authors.initials: J
authors.lastname: Mizon
authors.forename: P
authors.initials: P
authors.lastname: Degand
mesh_terms: D000293:Adolescent; D000328:Adult; D001798:Blood Proteins / Q000032:analysis*; D003424:Crohn Disease / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005243:Feces / Q000032:analysis*; D005260:Female; D006801:Humans; D007123:Immunoelectrophoresis, Two-Dimensional; D008297:Male; D008875:Middle Aged; D008970:Molecular Weight; D000515:alpha 1-Antitrypsin
chemical_list: D001798:Blood Proteins; D000515:alpha 1-Antitrypsin