Behaviour of plasmalogens during high-performance liquid chromatography on a silica column with a mobile phase containing phosphoric acid.
Silica high-performance liquid chromatographic separation of phospho- and sphingolipids of biological origin using a mobile phase containing phosphoric acid leads to gradual hydrolysis of plasmalogens during their passage through the column. The resulting 2-acyl lyso analogues give rise to peaks that tail in the direction of the parent intact plasmalogen. Tailing can be prevented by previous complete acid hydrolysis of plasmalogens. Direct high-performance liquid chromatographic profiling of phospholipids, their plasmalogens (as 2-acyl lyso analogues) and sphingolipids is probably the method of choice for the diagnosis of patients with deficient plasmalogen biosynthesis caused by peroxisomal abnormalities.
Hoving
E B
EB
Central Laboratory for Clinical Chemistry, University Hospital, Groningen, The Netherlands.
Prins
J
J
Rutgers
H M
HM
Muskiet
F A
FA
D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007202:Indicators and Reagents; D010743:Phospholipids / Q000097:blood; D010756:Phosphoric Acids; D010955:Plasmalogens / Q000097:blood*; D013107:Sphingolipids / Q000097:blood
D007202:Indicators and Reagents; D010743:Phospholipids; D010756:Phosphoric Acids; D010955:Plasmalogens; D013107:Sphingolipids