Trichokirin, a ribosome-inactivating protein from the seeds of Trichosanthes kirilowii Maximowicz. Purification, partial characterization and use for preparation of immunotoxins.
A protein, here named trichokirin, was extracted from the seeds of Trichosanthes kirilowii and purified by ion-exchange and gel-filtration chromatography. Trichokirin is a basic glycoprotein of apparent relative molecular mass of 27,000 with a strong ribosome-inactivating activity. Alignment of the trichokirin, trichosanthin and momordin N-terminal sequences shows a substantial degree of homology. Trichokirin was conjugated to a monoclonal antibody directed against the Thy 1.2 antigen with the cleavable dimethyl 3,3'-dithiobispropionimidate cross-linking reagent. This immunotoxin selectively killed leukemia cells expressing the Thy 1.2 antigen. The addition of ammonium chloride, which increases the cytotoxicity of ricin A-chain immunotoxins, blocks that of the trichokirin immunotoxin, suggesting that they enter cells by different mechanisms. In vivo studies showed that the pharmacokinetic properties of the trichokirin immunotoxin could be more advantageous than those of the ricin A-chain immunotoxins for in vivo applications.
Casellas
P
P
Sanofi Recherche, Centre de Montpellier, France.
Dussossoy
D
D
Falasca
A I
AI
Barbieri
L
L
Guillemot
J C
JC
Ferrara
P
P
Bolognesi
A
A
Cenini
P
P
Stirpe
F
F
D000595:Amino Acid Sequence; D000643:Ammonium Chloride / Q000494:pharmacology; D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002845:Chromatography / Q000379:methods; D002850:Chromatography, Gel; D004357:Drug Synergism; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000302:isolation & purification*; D000922:Immunotoxins / Q000302:isolation & purification* / Q000633:toxicity; D007942:Leukemia, Experimental / Q000378:metabolism; D008657:Metabolic Clearance Rate / Q000187:drug effects; D051379:Mice; D008969:Molecular Sequence Data; D008985:Monensin / Q000494:pharmacology; D037121:Plant Lectins; D010940:Plant Proteins / Q000302:isolation & purification*; D054789:Ribosome Inactivating Proteins, Type 1; D012270:Ribosomes / Q000187:drug effects / Q000276:immunology*; D012276:Ricin / Q000633:toxicity; D012639:Seeds / Q000032:analysis*
D006023:Glycoproteins; D000922:Immunotoxins; D037121:Plant Lectins; D010940:Plant Proteins; D054789:Ribosome Inactivating Proteins, Type 1; C057422:trichokirin protein, Trichosanthes kirilowii; D000643:Ammonium Chloride; D012276:Ricin; D008985:Monensin