Immunohistochemical staining for terminal deoxynucleotidyl transferase (TDT). An enhanced method in routinely processed formalin-fixed tissue sections.
This report describes an improved technique for sensitive and specific localization of terminal deoxynucleotidyl transferase (TdT) in routinely processed paraffin-embedded, formalin-fixed tissue sections using DNAse pretreatment and the avidin-biotin complex (ABC) technique. This method is useful in identifying lymphoblastic lymphomas (14/15 cases positive), with all other B- and T-cell lymphomas tested negative for the reaction. Used in conjunction with monoclonal antibodies immunoreactive for T- and B-cells in paraffin sections this technique should prove helpful in immunophenotyping malignant lymphomas where fresh tissue is unavailable for study.
Said
J W
JW
Department of Pathology and Laboratory Medicine, Cedars Sinai Medical Center, Los Angeles, California 90048.
Shintaku
I P
IP
Pinkus
G S
GS
D001402:B-Lymphocytes; D004796:Clinical Enzyme Tests; D004253:DNA Nucleotidylexotransferase / Q000032:analysis*; D004254:DNA Nucleotidyltransferases / Q000032:analysis*; D003851:Deoxyribonucleases; D005557:Formaldehyde; D006801:Humans; D007150:Immunohistochemistry; D008228:Lymphoma, Non-Hodgkin / Q000201:enzymology* / Q000473:pathology; D010232:Paraffin; D013601:T-Lymphocytes
D005557:Formaldehyde; D010232:Paraffin; D004254:DNA Nucleotidyltransferases; D004253:DNA Nucleotidylexotransferase; D003851:Deoxyribonucleases