Comparison between a solid-phase low-ionic-strength solution antiglobulin test and conventional low-ionic-strength antiglobulin test: assessment for the screening of antierythrocyte antibodies.
A solid-phase low-ionic strength salt antiglobulin test (LISS-SPAT) has been developed using a microplate coated with dried sera as a solid phase. Before coating, the in vitro C3d fragment generation was activated by adding heat-aggregated immunoglobulin. The LISS-SPAT was compared with low-ionic strength conventional antiglobulin test (LISS-AGT) and also with a test using polybrene or papain microplates. When detecting the IgG and IgM antierythrocyte antibodies the reaction was developed in the same way in LISS-SPAT and LISS-AGT. In routine work, the LISS-SPAT provides a fast, reliable, handy and inexpensive screening of antibodies. This method appears to be an additional method to the papain and polybrene tests in microplates.
Guignier
F
F
Centre Régional de Transfusion Sanguine de Dijon, France.
Domy
M
M
Angue
M
M
Richaud
P
P
Chatelain
P
P
D001789:Blood Group Antigens / Q000276:immunology*; D003176:Complement C3 / Q000032:analysis; D015933:Complement C3d; D003298:Coombs Test; D006801:Humans; D007518:Isoantibodies / Q000032:analysis*; D010206:Papain
D001789:Blood Group Antigens; D003176:Complement C3; D007518:Isoantibodies; D015933:Complement C3d; D010206:Papain