A simple method for preparation of molecular weight marker proteins for sodium dodecyl sulfate-polyacrylamide gel electrophoresis by photopolymerizations of hemoglobin subunits.
Photoirradiation of globin, obtained from human hemoglobin, in the presence of oxygen and protoporphyrin produced a series of polymers of globin subunits ranging from dimer to dodecamer. These polymers are useful as molecular weight markers because they cover a relatively wide range of molecular weights (Mr 15,500-186,000) with a constant and narrow interval of Mr 15,500.
Sato
H
H
Department of Perinatology, Institute for Developmental Research, Aichi Prefecture Colony, Japan.
Aono
S
S
Semba
R
R
Kashiwamata
S
S
D015415:Biomarkers / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D006454:Hemoglobins / Q000528:radiation effects; D006801:Humans; D008027:Light; D008722:Methods; D008970:Molecular Weight; D011108:Polymers; D011506:Proteins / Q000032:analysis*
D015415:Biomarkers; D006454:Hemoglobins; D011108:Polymers; D011506:Proteins