Definitive identification of human T cells in formalin fixed paraffin wax embedded tissue.
The use of a new monoclonal antibody, BF1, which reacts in routinely fixed tissues, directed against the beta chain of the T cell antigen receptor was assessed to determine its reactivity in a variety of normal tissues as well as in a number of B and T cell tumours and other lymphoid disorders. It reacted with all five of the unequivocal T cell tumours tested. BF1 will have widespread use in the assessment of lymphoid tissues in patients with the acquired immune deficiency syndrome (AIDS) and the differential diagnosis of tumours.
Francis
N D
ND
Pathology Department, St Mary's Hospital Medical School, London.
Clark
D M
DM
Boylston
A W
AW
D000163:Acquired Immunodeficiency Syndrome / Q000473:pathology; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D006801:Humans; D008198:Lymph Nodes / Q000473:pathology; D011948:Receptors, Antigen, T-Cell / Q000032:analysis; D013601:T-Lymphocytes / Q000276:immunology*
D000911:Antibodies, Monoclonal; D011948:Receptors, Antigen, T-Cell