title: Identification of calcium binding proteins in two-dimensional gel electrophoretic pattern of Drosophila thorax and their distribution in two types of muscles.
abstract: Ten Drosophila thorax proteins (six myosin light chains and four proteins called a, b, c, d) were found to have high affinities with Ca2+. This was proved after subjecting the total Drosophila thorax proteins to two-dimensional (2D) transblot, followed by 45Ca2+ autoradiography. Three proteins (a, c, d) showed Ca2+ dependent electrophoretic mobility changes. To know their tissue-specific localization, fibrillar and tubular type muscle fibers were individually dissected from freeze-dried flies and separately subjected to 2D gel electrophoresis. Fibrillar type muscle had protein b and a small amount of protein a. Tubular type muscle had proteins c, d and a very large amount of protein a. Protein d was characterized to be calmodulin.
authors.affiliation: Department of Physics, Faculty of Science, University of Tokyo.
authors.forename: Y
authors.initials: Y
authors.lastname: Tanaka
authors.forename: K
authors.initials: K
authors.lastname: Maruyama
authors.forename: T
authors.initials: T
authors.lastname: Mikawa
authors.forename: Y
authors.initials: Y
authors.lastname: Hotta
mesh_terms: D000818:Animals; D001345:Autoradiography / Q000379:methods; D002135:Calcium-Binding Proteins / Q000032:analysis*; D002147:Calmodulin / Q000032:analysis; D004330:Drosophila; D015180:Electrophoresis, Gel, Two-Dimensional; D009132:Muscles / Q000032:analysis*; D013909:Thorax
chemical_list: D002135:Calcium-Binding Proteins; D002147:Calmodulin