Conversion of the beckman liquid phase sequencer to a gas-liquid phase sequencer.
As an effective aid to extend the microsequencing capabilities the Beckman protein/peptide sequenator Series 890C has been successfully converted to a gas-liquid system, in which coupling buffer 25% trimethylamine was employed as a gas, and heptafluorobutyric acid as a liquid. The system has been found to be efficient for microsequencing (less than 100 pmol). The details of mechanical, plumbing, and other minor changes are described in this paper along with the results of sequencing proteins and peptides, directly and from blots.
Bhown
A S
AS
Department of Medicine, University of Alabama, Birmingham 35294.
Wayland
J L
JL
Lynn
J D
JD
Bennett
J C
JC
D000595:Amino Acid Sequence; D002623:Chemistry Techniques, Analytical / Q000295:instrumentation*; D002849:Chromatography, Gas / Q000295:instrumentation; D005466:Fluorocarbons; D007202:Indicators and Reagents; D008744:Methylamines; D012997:Solvents
D005466:Fluorocarbons; D007202:Indicators and Reagents; D008744:Methylamines; D012997:Solvents; C033094:perfluorobutyric acid; C023336:trimethylamine