Inhibitory effect of transforming growth factor-beta on epidermal growth factor-induced proliferation of cultured rat aortic smooth muscle cells.
This study was conducted to investigate the effect of transforming growth factor-beta (TGF-beta) on the proliferation of cultured rat aortic smooth muscle cells (SMCs). DNA synthesis, measured by the incorporation of [3H] thymidine, and the cell number of monolayered SMCs were measured after incubation with TGF-beta (1-100 ng/ml) in the presence or absence of epidermal growth factor (EGF; 100 ng/ml). TGF-beta alone did not affect DNA synthesis of SMCs. EGF significantly increased both DNA synthesis and cell number, while TGF-beta inhibited the increase in both in a dose-dependent manner without accompanying the significant cellular damage. These results indicate that TGF-beta exerts an inhibitory effect on the proliferation of cultured SMCs provoked by EGF.
Ouchi
Y
Y
Department of Geriatrics, Faculty of Medicine, University of Tokyo, Japan.
Hirosumi
J
J
Watanabe
M
M
Hattori
A
A
Nakamura
T
T
Orimo
H
H
D000818:Animals; D002455:Cell Division / Q000187:drug effects*; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D004815:Epidermal Growth Factor / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D004987:Ethers / Q000494:pharmacology; D015759:Ionomycin; D009131:Muscle, Smooth, Vascular / Q000166:cytology*; D017830:Octoxynol; D011092:Polyethylene Glycols / Q000494:pharmacology; D051381:Rats; D013997:Time Factors; D015291:Transforming Growth Factors / Q000494:pharmacology*
D004987:Ethers; D011092:Polyethylene Glycols; D015759:Ionomycin; D004815:Epidermal Growth Factor; D015291:Transforming Growth Factors; D017830:Octoxynol; D004247:DNA