title: Production of monospecific antisera by immunization with precipitin line leads to misinterpretation of immunoblot.
abstract: Immunoblotting displays an artifact in the preparation of monospecific antibodies from immunocomplexes cut out of line immunoelectrophoresis. Agarose induces the formation of antibodies towards a 68 KDa protein which is contained in commercial agarose.
authors.affiliation: Laboratoire de Photobiologie, U.E.R. des Sciences, Mont Saint Aignan, France.
authors.forename: A
authors.initials: A
authors.lastname: Aoues
authors.forename: C
authors.initials: C
authors.lastname: Huault
mesh_terms: D007106:Immune Sera; D007114:Immunization; D015151:Immunoblotting; D011233:Precipitin Tests
chemical_list: D007106:Immune Sera