title: [Cytotoxic action of the chemical substances found as admixtures in medical immunobiological preparations].
abstract: The cytotoxic action (CTA) of chemical substances contained as admixtures in medical immunobiological preparations on human diploid cells has been studied. Such chemical substances as rivanol and merthiolate in admissible concentrations show the highest degree of CTA. The results obtained in this investigation indicate that different concentrations of chemical substances may produce equal CTA; thus, thiolate in toxic in a dose of 0.8 microgram/ml; the same CTA is produced by aluminium sulfate in a concentration of 500 micrograms/ml. Small doses of chemical substances, producing no explicit manifestations of the cytotoxic effect, may have latent CTA determined by additional methods of investigation. CTA may be manifested as lethal, sublethal and latent cell lesions. In working out regulations on the test for CTA it is expedient to indicate admissible residual amounts of chemical substances contained in finished medical immunobiological preparations, considering that these amounts must be incapable of producing CTA in cell cultures. The conclusion has been made on the expediency of denoting small amounts of chemical substances capable of producing latent CTA as tentatively tolerable doses.
authors.forename: G P
authors.initials: GP
authors.lastname: Chervonskaia
authors.forename: A T
authors.initials: AT
authors.lastname: Kravchenko
authors.forename: V F
authors.initials: VF
authors.lastname: Runova
authors.forename: V M
authors.initials: VM
authors.lastname: Bedniagin
authors.forename: K N
authors.initials: KN
authors.lastname: Grinberg
mesh_terms: D001688:Biological Products / Q000633:toxicity*; D002478:Cells, Cultured; D004171:Diploidy; D004305:Dose-Response Relationship, Drug; D004340:Drug Contamination; D006801:Humans; D013997:Time Factors
chemical_list: D001688:Biological Products
vernacular_title: Tsitotoksicheskoe deĭstvie khimicheskikh veshchestv, soderzhashchikhsia v vide primeseĭ v nekotorykh meditsinskikh immunobiologicheskikh preparatakh.