Inhibition of in vitro mitogen-induced lymphoproliferative responses by sera from F344 rats with large granular lymphocyte leukemia.
The effect of large granular lymphocyte leukemia on F344 rat lymphocytes was studied by analyzing the blastogenic responses of normal spleen cells exposed to serum from leukemic rats. Sera from both transplanted as well as spontaneous cases of LGL leukemia markedly depressed the response to ConA and PHA. The suppressive activity was heat-stable at 56 degrees C, non-dialyzable and was effective even when added to lymphocytes only during the last 24 hrs of culture. A similar depression of blastogenesis was caused by sera from nonleukemic fasted rats. Depletion of lipoproteins from sera partially relieved the suppression. Leukemic and fasted rats had nearly identical serum lipoprotein electrophoretic profiles, indicating that abnormal lipoprotein metabolism may result from the severe anorexia which characterizes the terminal stages of the disease and may cause immunosuppression. Residual suppressive activity was also found in lipoprotein depleted sera. Supernatant fluids from spleen cell cultures of some leukemic rats also depressed lymphocyte blastogenic responses when compared to supernatants from normal spleen cultures.
Stromberg
P C
PC
Department of Veterinary Pathology, Texas A&M University, College Station 77843.
McMurray
D N
DN
Brown
C A
CA
D000818:Animals; D003208:Concanavalin A / Q000494:pharmacology; D066298:In Vitro Techniques; D007945:Leukemia, Lymphoid / Q000097:blood / Q000276:immunology*; D008074:Lipoproteins / Q000097:blood; D008213:Lymphocyte Activation; D008297:Male; D010835:Phytohemagglutinins / Q000494:pharmacology; D051381:Rats; D011916:Rats, Inbred F344; D013154:Spleen / Q000276:immunology; D013491:Suppressor Factors, Immunologic / Q000097:blood*; D013601:T-Lymphocytes / Q000276:immunology
D008074:Lipoproteins; D010835:Phytohemagglutinins; D013491:Suppressor Factors, Immunologic; D003208:Concanavalin A