Aromatase and nonaromatizing 10-demethylase activity of adrenal cortex mitochondrial P-450(11)beta.
19-Oxoandrostenedione, the product of 19-hydroxyandrostenedione by the 19-oxidase activity of the purified P-450(11)beta system of adrenal cortex mitochondria, was further oxidized and demethylated at the 10-position to give the C18-steroids, estrone (aromatase reaction) and 19-norandrostenedione (nonaromatizing 10-demethylase or C10-19 lyase reaction). These reactions, together with the initial hydroxylation of androstenedione at C19, form a sequence of P-450(11)beta-catalyzed C19-steroid 19-monooxygenase reactions. P-450(11)beta is thus similar to placental endoplasmic P-450AROM in some of its substrate specificity, but the two forms of P-450 appear to be different in both physiology and properties.
Suhara
K
K
Department of Chemistry, Faculty of Science, Kanazawa University, Japan.
Ohashi
K
K
Takahashi
K
K
Katagiri
M
M
D000302:Adrenal Cortex / Q000201:enzymology*; D000735:Androstenedione / Q000031:analogs & derivatives / Q000096:biosynthesis / Q000378:metabolism; D000818:Animals; D001141:Aromatase / Q000378:metabolism*; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D004970:Estrone / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D006900:Hydroxylation; D007700:Kinetics; D008928:Mitochondria / Q000201:enzymology*; D010088:Oxidoreductases / Q000378:metabolism; D013252:Steroid 11-beta-Hydroxylase / Q000378:metabolism*; D013250:Steroid Hydroxylases / Q000378:metabolism*
D004970:Estrone; D000735:Androstenedione; C014142:19-oxo-delta(4) androstene-3,17-dione; C011482:19-hydroxy-4-androstene-3,17-dione; D010088:Oxidoreductases; D013250:Steroid Hydroxylases; D001141:Aromatase; D013252:Steroid 11-beta-Hydroxylase; C002828:19-norandrostenedione