Oligonucleotide probes (TEM-1, OXA-1) versus isoelectric focusing in beta-lactamase characterization of 114 resistant strains.
Oligonucleotide probes specific for detection of the TEM-1 and OXA-1 beta-lactamase genes were compared with isoelectric focusing in 114 gram-negative beta-lactamase-producing strains representing at least 16 species. Correlations of 96 and 100% with isoelectric points were found for the TEM-1 and OXA-1 probes, respectively.
Ouellette
M
M
Département de Biochimie, Faculté des Sciences et de Génie, Université Laval, Ste-Foy, Quebec, Canada.
Paul
G C
GC
Philippon
A M
AM
Roy
P H
PH
D001419:Bacteria / Q000201:enzymology*; D004352:Drug Resistance, Microbial; D007525:Isoelectric Focusing; D009841:Oligonucleotides; D001618:beta-Lactamases / Q000032:analysis*
D009841:Oligonucleotides; D001618:beta-Lactamases