Interleukin 5 and interleukin 2 cooperate with interleukin 4 to induce IgG1 secretion from anti-Ig-treated B cells.
We have analyzed requirements for IL-4-induced secretion of IgG1 from anti-Ig-activated B cells. Activated B cell blasts prepared by culture of high density B cells with anti-Ig failed to secrete IgG1 upon subsequent culture with LPS and IL-4. However, IL-4 markedly suppressed IgM secretion in the same cultures. Addition of a mixture of T cell-derived lymphokines or rIL-5 to LPS-stimulated anti-Ig blasts restored IL-4-stimulated IgG1 secretion; rIL-2 further enhanced the response to IL-4 + rIL-5. These results suggest that IL-4, IL-5, and IL-2 cooperate in the regulation of B lymphocyte Ig isotype expression.
Purkerson
J M
JM
Department of Pharmacology, University of Virginia Medical School, Charlottesville 22908.
Newberg
M
M
Wise
G
G
Lynch
K R
KR
Isakson
P C
PC
D000818:Animals; D000888:Antibodies, Anti-Idiotypic; D001402:B-Lymphocytes / Q000276:immunology*; D004357:Drug Synergism; D005786:Gene Expression Regulation; D007074:Immunoglobulin G / Q000378:metabolism*; D007132:Immunoglobulin Isotypes / Q000378:metabolism*; D007075:Immunoglobulin M / Q000378:metabolism; D007376:Interleukin-2 / Q000008:administration & dosage*; D015847:Interleukin-4; D015848:Interleukin-5; D007378:Interleukins / Q000008:administration & dosage*; D008213:Lymphocyte Activation; D051379:Mice
D000888:Antibodies, Anti-Idiotypic; D007074:Immunoglobulin G; D007132:Immunoglobulin Isotypes; D007075:Immunoglobulin M; D007376:Interleukin-2; D015848:Interleukin-5; D007378:Interleukins; D015847:Interleukin-4