Analysis of the aqueous phase of human cervical mucus by reversed-phase high-performance liquid chromatography and capillary isotachophoresis.
The aqueous phase of human cervical mucus was analysed by reversed-phase high-performance liquid chromatography (HPLC) and capillary isotachophoresis (ITP). With HPLC, seventeen ultraviolet-absorbing and eight fluorescent components and with ITP five anionic and four cationic components could be determined. The sample pre-treatment consisted of a simple ultrafiltration. Ten samples from fertile women and eleven samples from infertile women were analysed. In six samples from the infertile group higher median concentrations of several components were found. This may be an indication of disturbances in the biochemical processes of the cervical mucus of woman with fertility problems.
Gladdines
M M
MM
Laboratory of Instrumental Analysis, Eindhoven University of Technology, The Netherlands.
Ackermans
M T
MT
Everaerts
F M
FM
Van der Linden
P J
PJ
Vader
H L
HL
Wiegerinck
M A
MA
D002582:Cervix Mucus / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D004586:Electrophoresis; D005260:Female; D006801:Humans; D007247:Infertility, Female / Q000378:metabolism; D007773:Lactates / Q000032:analysis; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
D007773:Lactates