Rabbit antisera to enterobacteriaceae isolated from HLA-B27 positive patients with ankylosing spondylitis (AS) are lytic for the mononuclear cells of AS patients.
Vaccines prepared from Gram-negative bacteria isolated from the stools of HLA-B27 positive AS patients were used to immunize rabbits. Three of the sera obtained were lytic in vitro for the mononuclear cells of HLA-B27 positive AS patients. One of these sera discriminated between AS patients and healthy HLA-B27 positive individuals. Cytolysis was determined in an automated, non-radioactive assay based on the release of carboxyfluorescein diacetate and the incorporation of propidium iodide.
Beukelman
C J
CJ
van Leeuwen
A
A
Aerts
P C
PC
van Kregten
E
E
van Bree
S
S
van Rood
J J
JJ
Willers
J M
JM
van Dijk
H
H
D000818:Animals; D003601:Cytotoxicity Tests, Immunologic; D004755:Enterobacteriaceae / Q000276:immunology*; D006680:HLA Antigens / Q000276:immunology; D015796:HLA-B27 Antigen; D006801:Humans; D007106:Immune Sera / Q000633:toxicity*; D007963:Leukocytes, Mononuclear / Q000187:drug effects*; D011817:Rabbits / Q000276:immunology; D013167:Spondylitis, Ankylosing / Q000097:blood* / Q000276:immunology
D006680:HLA Antigens; D015796:HLA-B27 Antigen; D007106:Immune Sera