Granulocyte precursors are the principal cells in bone marrow that stimulate allospecific cytolytic T-lymphocyte responses.
Mouse bone marrow cells were fractionated and enriched for functional activity as stimulators of allospecific cytolytic T-lymphocyte (CTL) responses in vitro. The relevant stimulator cells were enriched sequentially in the low-density fraction of bone marrow, its 2-hr adherent and 18-hr non-adherent fractions and in the FcR-negative fraction of 18-hr non-adherent cells. The functionally enriched cell population contained over 90% granulocyte precursors by ultrastructural analysis. The results indicate that granulocyte precursors are the principal cells in bone marrow that stimulate alloreactive T-cell responses.
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Mount Sinai Hospital Research Institute, University of Toronto, Ontario, Canada.
Axelrod
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Fernandes
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D000818:Animals; D001853:Bone Marrow / Q000276:immunology*; D002448:Cell Adhesion; D002469:Cell Separation; D002499:Centrifugation, Density Gradient; D006098:Granulocytes / Q000276:immunology* / Q000648:ultrastructure; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D011961:Receptors, Fc / Q000032:analysis; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D011961:Receptors, Fc