An immunohistochemical study performed with monoclonal and polyclonal antibodies to mouse epidermal growth factor.
We developed two monoclonal antibodies, E5 and F5, which react with mouse epidermal growth factor (mEGF) and urogastrone. These antibodies and a rabbit antiserum to mEGF were used for immunohistochemical analysis of staining reactions in rodent and human tissues. Our results do not confirm the published reports of EGF-like immunoreactivity in Brunner's glands, human submandibular glands, human kidney tissue, or rodent brain sections.
Beerstecher
H J
HJ
Department of Pathology, University of Leiden, The Netherlands.
Huiskens-van der Meij
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C
Warnaar
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D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001923:Brain Chemistry; D002011:Brunner Glands / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D004815:Epidermal Growth Factor / Q000276:immunology*; D006801:Humans; D006825:Hybridomas; D007106:Immune Sera / Q000276:immunology; D007118:Immunoassay; D007150:Immunohistochemistry; D007668:Kidney / Q000032:analysis; D051379:Mice; D051381:Rats; D013363:Submandibular Gland / Q000032:analysis
D000911:Antibodies, Monoclonal; D007106:Immune Sera; D004815:Epidermal Growth Factor