Improved method for multisample evaluation of ligand binding to cells. Application to IL-2 receptor measurement.
A newly developed assay using microtitre plates with removable wells permits rapid measurements of IL-2 receptors. Triplicate or quadruplicate samples of several ligand dilutions are easily handled, thus giving more reliable results. In addition, measurement of binding parameters may be accomplished simultaneously on several cell lines. Results are presented which show that the binding assay may also be used for measuring binding constants of antibodies.
Lindqvist
C
C
Department of Immunology, Karolinska Institute, Stockholm, Sweden.
Mogensen
E L
EL
Koman
A
A
D000818:Animals; D000911:Antibodies, Monoclonal; D002460:Cell Line; D006801:Humans; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology / Q000378:metabolism; D051379:Mice; D011869:Radioligand Assay / Q000295:instrumentation / Q000379:methods; D015375:Receptors, Interleukin-2 / Q000032:analysis* / Q000096:biosynthesis; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology / Q000378:metabolism
D000911:Antibodies, Monoclonal; D015375:Receptors, Interleukin-2