title: Tyrosine phosphorylation of the human T cell antigen receptor zeta-chain: activation via CD3 but not CD2.
abstract: TCR stimulation by Ag or anti-receptor antibodies in murine T cells results in the activation of two independent protein kinases, protein kinase C (PKC) and a protein tyrosine kinase. Similarly, stimulation of murine Thy-1 or Ly-6 with mAb also results in activation of both of these kinase pathways. Tyrosine phosphorylation in all cases occurs on the TCR zeta-chain. It is known that Ag and anti-receptor antibodies activate PKC in human T cells. In this study we demonstrate that mitogen or anti-CD3 antibodies activate tyrosine phosphorylation of the human TCR-zeta-chain. PMA, which activates PKC, does not result in zeta-chain tyrosine phosphorylation. Stimulation of human T cells by antibodies that bind the CD2 molecule is an alternate mode of inducing T cell proliferation. These antibodies surprisingly do not induce tyrosine phosphorylation of the zeta-chain. Thus, different methods of cellular activation can result in distinguishable patterns of receptor-mediated biochemical signaling events.
authors.affiliation: Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, Bethesda, MD 20892.
authors.forename: A M
authors.initials: AM
authors.lastname: Weissman
authors.forename: P
authors.initials: P
authors.lastname: Ross
authors.forename: E T
authors.initials: ET
authors.lastname: Luong
authors.forename: P
authors.initials: P
authors.lastname: Garcia-Morales
authors.forename: M L
authors.initials: ML
authors.lastname: Jelachich
authors.forename: W E
authors.initials: WE
authors.lastname: Biddison
authors.forename: R D
authors.initials: RD
authors.lastname: Klausner
authors.forename: L E
authors.initials: LE
authors.lastname: Samelson
mesh_terms: D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology*; D006801:Humans; D008213:Lymphocyte Activation; D008565:Membrane Proteins; D008970:Molecular Weight; D010766:Phosphorylation; D011233:Precipitin Tests; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D011948:Receptors, Antigen, T-Cell / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D013601:T-Lymphocytes / Q000201:enzymology / Q000276:immunology / Q000378:metabolism*; D014443:Tyrosine / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D008565:Membrane Proteins; D011948:Receptors, Antigen, T-Cell; C054979:antigen T cell receptor, zeta chain; D014443:Tyrosine; D011505:Protein-Tyrosine Kinases