title: Measles virus fusion protein presented in an immune-stimulating complex (iscom) induces haemolysis-inhibiting and fusion-inhibiting antibodies, virus-specific T cells and protection in mice.
abstract: Immune-stimulating complexes (iscoms), which have recently been shown to be highly effective for the antigenic presentation of membrane proteins of viruses, were prepared with affinity-purified fusion (F) protein of measles virus (MV), using an adaptation of the standard method for iscom preparation. Immunization of monkeys with the F iscom preparation induced biologically active anti-F protein antibodies as was shown in haemolysis inhibition and cell-cell fusion inhibition tests. A whole MV iscom preparation, which also contained the haemagglutinin protein, induced not only also haemolysis-inhibiting antibodies, but, in contrast to the F iscom preparation, also haemagglutination-inhibiting and virus-neutralizing antibodies. In addition the F iscom preparation was shown to activate measles virus-specific T cells in mice. This was demonstrated by the generation of an MV-specific delayed type hypersensitivity response in F iscom-immunized animals and by the isolation of T cell clones specific for MV F protein with the T helper phenotype. Vaccination of mice with MV iscom or F iscom protected them from MV-induced fatal encephalopathy. The data concerning the immunogenicity of MV proteins presented in iscoms are discussed in relation to their potential for the development of an inactivated measles vaccine.
authors.affiliation: Department of Immunobiology, National Institute of Public Health and Environmental Hygiene, Bilthoven, The Netherlands.
authors.forename: P
authors.initials: P
authors.lastname: de Vries
authors.forename: R S
authors.initials: RS
authors.lastname: van Binnendijk
authors.forename: P
authors.initials: P
authors.lastname: van der Marel
authors.forename: A L
authors.initials: AL
authors.lastname: van Wezel
authors.forename: H O
authors.initials: HO
authors.lastname: Voorma
authors.forename: B
authors.initials: B
authors.lastname: Sundquist
authors.forename: F G
authors.initials: FG
authors.lastname: Uytdehaag
authors.forename: A D
authors.initials: AD
authors.lastname: Osterhaus
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis*; D005260:Female; D007114:Immunization; D008252:Macaca fascicularis; D008459:Measles virus / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008823:Micelles; D062605:Quillaja Saponins; D011817:Rabbits; D012503:Saponins; D013601:T-Lymphocytes / Q000276:immunology*; D014760:Viral Fusion Proteins / Q000008:administration & dosage / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D008823:Micelles; D062605:Quillaja Saponins; D012503:Saponins; D014760:Viral Fusion Proteins; C046386:Quil A