Lymphokine mRNA and T cell multireceptor mRNA of the Ig super gene family are reciprocally modulated during human T cell activation.
The expression of genes, implicated in T cell proliferation as well as those coding for the three chains of the T cell receptor (TcR) and for the CD4 and CD8 molecules, has been investigated during activation of various T cell populations maintained in long-term culture. We show that a minimal activation signal, capable of inducing interleukin 2 (IL 2) receptor expression without the expression of IL2 and interferon-gamma, was not associated with a down-regulation of the messengers coding for the T cell multireceptor molecules. In contrast, a strong activation signal via the TcR/CD3 complex or via the CD2 molecule resulted in a reciprocal transient up-regulation of the messengers implicated in T cell proliferation and a down-regulation of the messengers encoding the molecules of the TcR complex of the immunoglobulin super gene family.
Paillard
F
F
INSERM U 152, CNRS UA 628, Hôpital Cochin, Paris, France.
Sterkers
G
G
Bismuth
G
G
Gomard
E
E
Vaquero
C
C
D000945:Antigens, Differentiation, T-Lymphocyte / Q000235:genetics*; D015152:Blotting, Northern; D005786:Gene Expression Regulation; D006801:Humans; D008213:Lymphocyte Activation; D008222:Lymphokines / Q000235:genetics*; D005810:Multigene Family; D012333:RNA, Messenger / Q000235:genetics; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*; D015375:Receptors, Interleukin-2 / Q000235:genetics; D013601:T-Lymphocytes / Q000502:physiology*
D000945:Antigens, Differentiation, T-Lymphocyte; D008222:Lymphokines; D012333:RNA, Messenger; D011948:Receptors, Antigen, T-Cell; D015375:Receptors, Interleukin-2