title: Molecular weight of tumor necrosis factor determined by gel permeation chromatography alone or in combination with low-angle laser light scattering.
abstract: The molecular weight of human recombinant tumor necrosis factor was determined at neutral pH by gel permeation chromatography alone or in combination with low-angle laser light scattering. Mean values of 39,200 +/- 800 and 48,800 +/- 900, respectively, were obtained by the two analyses. The results resolve the apparent discrepancy in the reported values of the molecular weight of this cytokine, confirming that it exists as a trimer in neutral solution with a molecular weight of about 50,000.
authors.affiliation: Institute for Enzyme Research, School of Medicine, University of Tokushima, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Yoshimura
authors.forename: S
authors.initials: S
authors.lastname: Sone
authors.forename: S
authors.initials: S
authors.lastname: Maezawa
authors.forename: K
authors.initials: K
authors.lastname: Kameyama
authors.forename: T
authors.initials: T
authors.lastname: Takagi
mesh_terms: D002850:Chromatography, Gel; D007834:Lasers; D008027:Light; D008970:Molecular Weight; D012542:Scattering, Radiation; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis*
chemical_list: D014409:Tumor Necrosis Factor-alpha