Induction of colony-stimulating factor and stimulation of stem cell proliferation by injection of muroctasin.
Modulation of myelopoiesis by anomeric mixture of N2-[(N-acetylmuramoyl)-L-alanyl-D-isoglutamyl]-N6-stearoyl-L-lysin e (MDP-Lys(L18), muroctasin), a muramyl dipeptide analog, was investigated in mice. When BDF1 mice were subcutaneously treated with a single dose of 100 micrograms of MDP-Lys(L18), an increase in the number of peripheral blood leukocytes, a rise in the serum levels of colony-stimulating factor (CSF), proliferation of multipotential stem cells in the bone marrow and the spleen, and an expansion of granulocyte-macrophage progenitors in the bone marrow were observed. These findings suggest that the increase in the number of peripheral blood leukocytes in BDF1 mice by MDP-Lys(L18) is due to CSF production and stimulation of stem cell proliferation.
Yamaguchi
F
F
Research Institute, Daiichi Seiyaku Co., Ltd., Tokyo, Japan.
Akasaki
M
M
Tsukada
W
W
D000119:Acetylmuramyl-Alanyl-Isoglutamine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D003115:Colony-Stimulating Factors / Q000096:biosynthesis*; D005260:Female; D006410:Hematopoiesis / Q000187:drug effects; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000187:drug effects*; D007958:Leukocyte Count / Q000187:drug effects; D008214:Lymphocytes; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009000:Monocytes; D009504:Neutrophils
D000276:Adjuvants, Immunologic; D003115:Colony-Stimulating Factors; D000119:Acetylmuramyl-Alanyl-Isoglutamine; C036866:romurtide