Sensitive determination of D-lactic acid in biological samples by high-performance liquid chromatography.
D-Lactate in biological samples was converted into the hydrazone of pyruvate in the presence of D-lactate dehydrogenase, an NADH-reoxidation system using diaphorase, DL-6,8-thioctamide and hydrazine. The hydrazone was converted into 2-methylquinoxanol by o-phenylenediamine in hydrochloric acid, and then the quinoxanol was determined by high-performance liquid chromatography with fluorescence detection. The calibration curve of D-lactate was linear up to at least 60 nmol/ml, and the determination limit was 600 fmol. Using this method, D-lactate was determined in biological samples.
Ohmori
S
S
Faculty of Pharmaceutical Sciences, Okayama University, Japan.
Iwamoto
T
T
D000328:Adult; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003921:Diabetes Mellitus, Experimental / Q000097:blood; D006801:Humans; D006834:Hydrazines; D007773:Lactates / Q000032:analysis* / Q000097:blood / Q000652:urine; D019344:Lactic Acid; D008297:Male; D011773:Pyruvates / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D013056:Spectrophotometry, Ultraviolet; D015014:Yogurt / Q000032:analysis
D006834:Hydrazines; D007773:Lactates; D011773:Pyruvates; D019344:Lactic Acid