Capacity of purified unprimed Lyt-2+ cells to reject Ia- H-2-different tumor cells in the Winn assay.
To examine which cells participate in primary anti-H-2 responses to Ia- tumors in vivo, irradiated mice were injected intracutaneously with small doses of tumor cells mixed with purified populations of host-type lymphoid cells. Studies with three different Ia- H-2-different tumors showed that purified unprimed Lyt-2+ cells were highly efficient at suppressing tumor growth. Lyt-2+ cells were appreciably more effective at suppressing tumor growth than unseparated T cells, and no protection was seen with injection of L3T4+ cells (except in the late states of tumor growth). It is suggested that class I alloantigens on the tumors are directly immunogenic for Lyt-2+ cells. Without need for help from L3T4+ cells, the responding Lyt-2+ cells rapidly differentiate into cytotoxic cells and destroy the tumor cells before macroscopic tumors can arise.
Sprent
J
J
Department of Immunology, Research Institute of Scripps Clinic, La Jolla, CA 92037.
Schaefer
M
M
D000818:Animals; D000950:Antigens, Ly / Q000276:immunology*; D001681:Biological Assay; D006183:H-2 Antigens / Q000276:immunology*; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D007111:Immunity, Cellular; D051379:Mice; D009374:Neoplasms, Experimental / Q000276:immunology / Q000473:pathology; D011828:Radiation Chimera; D013601:T-Lymphocytes / Q000276:immunology*
D000950:Antigens, Ly; D006183:H-2 Antigens; D000949:Histocompatibility Antigens Class II