title: 5S rRNA genes in Pisum: sequence, long range and chromosomal organization.
abstract: We have employed a combination of techniques to examine the organization of pea 5S rRNA genes. These include the analysis of length variant interspersion patterns in cosmid clones, sequence analysis, Southern analysis of both conventional gels and field inversion gels and in situ hybridization. From these analyses we conclude that the 5S rRNA genes of pea are arranged in three major tandem arrays which are represented by three large EcoRI fragments and that these correspond to the three sites of in situ hybridization in the haploid pea complement.
authors.affiliation: The John Innes Institute, Norwich, England.
authors.forename: T H
authors.initials: TH
authors.lastname: Ellis
authors.forename: D
authors.initials: D
authors.lastname: Lee
authors.forename: C M
authors.initials: CM
authors.lastname: Thomas
authors.forename: P R
authors.initials: PR
authors.lastname: Simpson
authors.forename: W G
authors.initials: WG
authors.lastname: Cleary
authors.forename: M A
authors.initials: MA
authors.lastname: Newman
authors.forename: K W
authors.initials: KW
authors.lastname: Burcham
mesh_terms: D001483:Base Sequence; D002874:Chromosome Mapping; D004275:DNA, Ribosomal / Q000235:genetics; D007887:Fabaceae / Q000235:genetics; D005796:Genes; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010944:Plants / Q000235:genetics*; D010946:Plants, Medicinal; D012335:RNA, Ribosomal / Q000235:genetics*; D012341:RNA, Ribosomal, 5S / Q000235:genetics* / Q000302:isolation & purification; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
chemical_list: D004275:DNA, Ribosomal; D012335:RNA, Ribosomal; D012341:RNA, Ribosomal, 5S