Effect of interleukin 1 beta on transducing mechanisms in 235-1 clonal pituitary cells. Part I: Modulation of adenylate cyclase activity.
In the present study we evaluated the effect of interleukin 1-beta on adenylate cyclase activity in 235-1 pituitary cell line. The dose-response curve of interleukin 1 beta effect on adenylate cyclase activity showed a significant inhibition of basal enzyme activity at 1 pM concentration, while the inhibition of forskolin stimulated adenylate cyclase activity was more pronounced and evident at both 0.01 and 1 pM concentrations. The action of the monokine on basal enzyme activity was almost completely reverted by polyclonal anti interleukin 1 beta antibody. The incubation of the cells for 48h with interleukin 1 beta showed a different pattern of response. The inhibitory effect of interleukin 1 beta on adenylate cyclase activity disappeared, while the highest concentration of interleukin 1 beta tested, caused a meaningful stimulation of adenylate cyclase activity which is not present in acute condition. These data show that interleukin 1-beta interacts with the cAMP-generating system in the 235-1 clonal pituitary cells.
Schettini
G
G
Dept. of Pharmacology, II Medical School, University of Naples, Italy.
Florio
T
T
Meucci
O
O
Scala
G
G
Landolfi
E
E
Grimaldi
M
M
D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D002999:Clone Cells / Q000187:drug effects; D005576:Colforsin / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D007375:Interleukin-1 / Q000494:pharmacology*; D010902:Pituitary Gland / Q000187:drug effects*; D051381:Rats; D015290:Second Messenger Systems / Q000187:drug effects*
D007375:Interleukin-1; D005576:Colforsin; D000262:Adenylyl Cyclases