title: Flow cytometric quantitation of T cell phenotypes in cerebrospinal fluid and peripheral blood of homosexual men with and without antibodies to human immunodeficiency virus, type I.
abstract: Two-color flow cytometry was used to analyze T cell subsets (total (CD3), helper-inducer (CD4), and suppressor-cytotoxic (CD8] in paired specimens of cerebrospinal fluid (CSF) and peripheral blood of 66 homosexual men, including 62 with antibodies to human immunodeficiency virus, type 1 (HIV-1). With the exception of one traumatic specimen, all of the CSF specimens, 52 of which had less than or equal to 5 lymphocytes/mm3, were evaluated fully, with the number of lymphocytes counted for each antibody ranging from 200 to 2933 (mean = 1129). Proportions of CD3, CD4, and CD8 lymphocytes in CSF were very highly correlated with the proportions of these cells in the peripheral blood (r = 0.87, 0.96, and 0.94, respectively), as was the CD4/CD8 ratio (r = 0.98). These strong correlations were present in each of seven subgroups of study subjects defined on the basis of detailed neurologic examination, neuropsychological testing, and the presence or absence of antibodies to HIV-1. In the population studied, T cell phenotypes in CSF as analyzed by two-color flow cytometry were largely determined by the corresponding proportions in the peripheral blood.
authors.affiliation: Department of Environmental Health Sciences, Johns Hopkins Medical Institutions, Baltimore, MD 21205.
authors.forename: J B
authors.initials: JB
authors.lastname: Margolick
authors.forename: J C
authors.initials: JC
authors.lastname: McArthur
authors.forename: E R
authors.initials: ER
authors.lastname: Scott
authors.forename: J H
authors.initials: JH
authors.lastname: McArthur
authors.forename: S
authors.initials: S
authors.lastname: Cohn
authors.forename: H
authors.initials: H
authors.lastname: Farzadegan
authors.forename: B F
authors.initials: BF
authors.lastname: Polk
mesh_terms: D000328:Adult; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D001773:Blood Cells / Q000502:physiology*; D002555:Cerebrospinal Fluid / Q000166:cytology*; D005434:Flow Cytometry; D006679:HIV Seropositivity / Q000503:physiopathology*; D006716:Homosexuality; D006801:Humans; D008297:Male; D010641:Phenotype; D013601:T-Lymphocytes / Q000276:immunology / Q000502:physiology*
chemical_list: D000945:Antigens, Differentiation, T-Lymphocyte