Purification of 6-phosphogluconolactonase from bass (Dicentrarchus labrax L.)liver.
The enzyme 6-phosphogluconolactonase (EC 3.1.1.31) is present at high levels in bass liver. The enzyme has been purified 1253-fold with an overall yield of 78% in a procedure involving dye-ligand and gel filtration chromatographies. The purified enzyme which is homogenous by all the usual criteria has a molecular weight of about 30,000. It exhibits a considerably high catalytic efficiency with Kcat/Km of 8.5 x 10(7) M-1.s-1 at 22 degrees C. Its activity illustrates the importance of glucose oxidation via the pentose phosphate pathway relative to glycolysis in this organism.
Medina-Puerta
M M
MM
Departamento de Bioquímica. Facultad de Veterinaria. Universidad Complutense, Madrid, Spain.
Gallego-Iniesta
M
M
Garrido-Pertierra
A
A
D000818:Animals; D001492:Bass / Q000378:metabolism*; D002265:Carboxylic Ester Hydrolases / Q000032:analysis / Q000302:isolation & purification*; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006868:Hydrolysis; D008099:Liver / Q000201:enzymology*; D010473:Perciformes / Q000378:metabolism*; D011506:Proteins / Q000032:analysis
D011506:Proteins; D002265:Carboxylic Ester Hydrolases; C022363:6-phosphogluconolactonase