Hemolysis of chicken erythrocytes by t-butyl hydroperoxide and protection by plasma.
Chicken erythrocytes treated with 100-microM t-butyl hydroperoxide (t-BHP) were more than 80% hemolyzed in 2 h and more than 90% hemolyzed after 4 h of incubation. Autologous plasma retarded the rate of hemolysis of chicken red cells produced by t-BHP. Dialysis of chicken plasma resulted in a marked decrease in the ability of plasma to protect erythrocytes from hemolysis by t-BHP. Low molecular weight components of plasma such as uric acid are hypothesized to account for much of the dialyzable material that protects red cells from hemolysis.
Smith
R C
RC
Department of Animal and Dairy Sciences, Alabama Agricultural Experiment Station, Auburn Univerity 36849.
Gore
J Z
JZ
Roland
D
D
D000818:Animals; D002645:Chickens / Q000097:blood*; D004912:Erythrocytes / Q000187:drug effects*; D005260:Female; D006461:Hemolysis / Q000187:drug effects*; D010545:Peroxides / Q000494:pharmacology*; D010949:Plasma / Q000502:physiology*; D020122:tert-Butylhydroperoxide
D010545:Peroxides; D020122:tert-Butylhydroperoxide