title: Horizontal two-dimensional electrophoresis with immobilized pH gradients using PhastSystem.
abstract: Protocols for horizontal two-dimensional electrophoresis with immobilized pH gradients in the first dimension were modified for horizontal micro two-dimensional electrophoresis using PhastSystem. Different equilibration conditions of the first-dimensional immobilized pH gradient gel strip prior to second-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis were evaluated. Silver stained two-dimensional patterns were obtained within 3.5 h.
authors.affiliation: Lehrstuhl für Allgemeine Lebensmitteltechnologie, Technische Universität München, Freising-Weihenstephan, Federal Republic of Germany.
authors.forename: A
authors.initials: A
authors.lastname: Görg
authors.forename: W
authors.initials: W
authors.lastname: Postel
authors.forename: S
authors.initials: S
authors.lastname: Günther
authors.forename: C
authors.initials: C
authors.lastname: Friedrich
mesh_terms: D015180:Electrophoresis, Gel, Two-Dimensional / Q000295:instrumentation / Q000379:methods*; D006863:Hydrogen-Ion Concentration