Human recombinant interleukin-1 regulates cellular mRNA levels of dermatan sulphate proteoglycan core protein.
Human skin fibroblasts were exposed to various concentrations (from 0.01 to 5.0 units/ml) of human recombinant interleukin-1 alpha and interleukin-1 beta (IL-1 alpha and IL-1 beta). Both IL-1 alpha and IL-1 beta were found to increase dermatan-sulphate-proteoglycan (DSPG) core-protein mRNA levels. Maximal increase (3.0-fold) was seen at 48 h after addition of 1 unit of IL-1 beta/ml. In spite of the elevated DSPG-core-protein mRNA only a slight increase (from 10 to 18%) could be seen in the production of DSPG to cell-culture medium. No changes in the molecular mass of DSPG could be detected.
Heino
J
J
Department of Medical Biochemistry, University of Turku, Finland.
Kähäri
V M
VM
Mauviel
A
A
Krusius
T
T
D053674:Aggrecans; D002478:Cells, Cultured; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D016326:Extracellular Matrix Proteins; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D006023:Glycoproteins / Q000096:biosynthesis*; D007375:Interleukin-1 / Q000494:pharmacology*; D037181:Lectins, C-Type; D011509:Proteoglycans; D012333:RNA, Messenger / Q000378:metabolism*; D011994:Recombinant Proteins / Q000494:pharmacology*; D012867:Skin / Q000187:drug effects / Q000378:metabolism
D053674:Aggrecans; D016326:Extracellular Matrix Proteins; D006023:Glycoproteins; D007375:Interleukin-1; D037181:Lectins, C-Type; D011509:Proteoglycans; D012333:RNA, Messenger; D011994:Recombinant Proteins