On-line high-performance liquid chromatography-post-column reaction-capillary gas chromatography analysis of lipids in biological samples.
The versatility of on-line liquid chromatography and gas chromatography is further expanded by the addition of on-line derivatization. The on-line fractionation, derivatization and separation system is applied to the characterization of lipids in biological samples. Separation of the triglycerides from the phospholipids was accomplished on a narrowbore (2.0 mm I.D.) 5 microns silica column. The entire triglyceride fraction was transferred to a heated fixed bed reactor for esterification of the fatty acid constituents. Transfer of the derivatized zones to the gas chromatograph was accomplished by the use of a retention gap. Application of the system to the separation and characterization of Staphylococcus aureus is presented.
Raglione
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E. R. Squibb and Sons, New Brunswick, NJ 08903.
Hartwick
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D002849:Chromatography, Gas / Q000295:instrumentation; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation; D002855:Chromatography, Thin Layer; D007700:Kinetics; D008055:Lipids / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet; D013211:Staphylococcus aureus / Q000032:analysis*
D008055:Lipids