Intraretinal transplantation of fluorescently labeled retinal cell suspensions.
Dissociated cell suspensions of neonatal neural retina, labeled with the fluorescent dyes Fast blue or Fluoro-gold, were transplanted into the retina of normal adult rats or of rats affected by late stage phototoxic retinopathy. Light microscopy showed good survival, differentiation, and integration of the transplants, as well as permanence of the label up to 100 days. The results indicate that the transplantation of dissociated, fluorescently labeled retinal cells has a number of advantages over the transplantation of solid fragments of retinal tissue, previously performed by ourselves and others. The following are some of the most immediate procedural advantages: the number of transplanted cells can be assessed, the transplanted cells are in a more intimate contact with the host tissue and therefore integrate better with the host, and the fluorescent tags permit precise determination of the survival and distribution of the transplanted cells.
del Cerro
M
M
Department of Neurobiology and Anatomy, University of Rochester Medical School, NY 14642.
Notter
M F
MF
Wiegand
S J
SJ
Jiang
L Q
LQ
del Cerro
C
C
D000578:Amidines; D000818:Animals; D002469:Cell Separation; D005456:Fluorescent Dyes; D006085:Graft Survival; D066298:In Vitro Techniques; D051381:Rats; D011917:Rats, Inbred Lew; D012160:Retina / Q000166:cytology / Q000502:physiology / Q000637:transplantation*; D013266:Stilbamidines
C049774:2-hydroxy-4,4'-diamidinostilbene, methanesulfonate salt; D000578:Amidines; D005456:Fluorescent Dyes; D013266:Stilbamidines; C031455:diamidino compound 253-50