Photoaffinity labelling with [3H]R1881 of androgen receptors from human cultured genital fibroblasts of normal individuals and patients with androgen receptor abnormalities.
Androgen receptors were partially purified by affinity chromatography of cytosols prepared from cultured genital fibroblasts of normal individuals and patients with androgen receptor abnormalities. Partially purified receptors were covalently labelled with [3H]R1881 (triated methyltrienolone) by ultraviolet photoactivation. Gel electrophoresis of cytosols from normal individuals showed a single radioactive peak, Mr approximately 97 K. Cytosols from patients with decreased nuclear transfer showed a similar peak, Mr approximately 97 K; cytosols from patients with partial androgen insensitivity (PAIS) or receptor positive complete androgen insensitivity (CAIS, R+) showed a peak Mr approximately 30 K-43 K.
Gyorki
S
S
Medical Research Centre, Prince Henry's Hospital, Melbourne, Australia.
Warne
G L
GL
Funder
J W
JW
D000345:Affinity Labels; D002478:Cells, Cultured; D003600:Cytosol / Q000378:metabolism; D004963:Estrenes / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D007021:Hypospadias / Q000378:metabolism*; D015741:Metribolone; D011944:Receptors, Androgen / Q000302:isolation & purification / Q000378:metabolism*; D012016:Reference Values; D012867:Skin / Q000378:metabolism; D045165:Testosterone Congeners / Q000378:metabolism*
D000345:Affinity Labels; D004963:Estrenes; D011944:Receptors, Androgen; D045165:Testosterone Congeners; D015741:Metribolone