Identification of interleukin-1 in normal human lymph derived from skin.
Interleukin 1 was isolated from human lymph drained from normal skin. The lymph was separated by gel filtration chromatography on Spherogel TSK SW 2000 column. IL-1 activity was found in three fractions: greater than 70 kD, 13-16 kD and 5-5.5 kD. Additionally a slight IL-1 activity was found in the 36-40 kD fraction. When exogenous IL-1 was added to the lymph inhibitory activity was detected in fractions: greater than 70 kD, 22-30 kD and 5.5-8 kD.
Plachta
J
J
Department of Surgical Research & Transplantology, Polish Academy of Sciences, Warsaw.
Olszewski
W L
WL
Grzelak
I
I
Engeset
A
A
Cholewka
W
W
D000293:Adolescent; D000328:Adult; D000818:Animals; D001681:Biological Assay; D002455:Cell Division / Q000187:drug effects; D006801:Humans; D007375:Interleukin-1 / Q000302:isolation & purification* / Q000502:physiology; D008196:Lymph / Q000032:analysis*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008970:Molecular Weight; D012867:Skin / Q000032:analysis*; D013347:Subcellular Fractions / Q000032:analysis; D013950:Thymus Gland / Q000166:cytology / Q000187:drug effects
D007375:Interleukin-1