Determination of amiodarone hydrochloride in pharmaceutical formulations by derivative UV spectrophotometry and high-performance liquid chromatography (HPLC).
Assay procedures based on derivative ultraviolet spectrophotometry and high-performance liquid chromatography (HPLC) have been developed for the specific determination of amiodarone hydrochloride in pharmaceutical dosage forms. The use of first- and second-order derivative spectrophotometry was found to have suppressed the background absorption from the excipients with comparable accuracy and precision to the reversed-phased HPLC reference method. A conventional UV absorption method (lambda = 242 nm) is subject to possible interference by formulation excipients.
Di Pietra
A M
AM
Department of Pharmaceutical Sciences, University of Bologna, Italy.
Cavrini
V
V
Gatti
R
R
Raggi
M A
MA
D000638:Amiodarone / Q000032:analysis*; D002214:Capsules; D002851:Chromatography, High Pressure Liquid; D013056:Spectrophotometry, Ultraviolet; D013607:Tablets
D002214:Capsules; D013607:Tablets; D000638:Amiodarone