Interleukin 1 promotes tumor cell adhesion to cultured human endothelial cells.
We report that IL 1 acts on the endothelium, inducing a long-lasting increase in its adhesivity to tumor cells. Selective pretreatment of cultured human umbilical vein endothelial cells (EC) with IL 1 caused a significant increase in adhesion of three human colorectal carcinoma (HT-29, HCC-P2988, and HCC-M1410) cell lines and one human melanoma (A-375) cell line. Tumor necrosis factor (TNF) was as effective as IL 1 in promoting tumor cell adhesion to EC, whereas IFN gamma and IL 2 were inactive. The IL 1 and TNF induction of EC adhesivity was both concentration (threshold concentration 1 U/ml) and time dependent (peak 4-6 h), reversible within 24 h, and blocked by a protein synthesis inhibitor. The IL 1 and TNF action on EC may play a role in tumor cell lodgement.
Dejana
E
E
Istituto di Ricerche Farmacologiche Mario Negri, Milan, Italy.
Bertocchi
F
F
Bortolami
M C
MC
Regonesi
A
A
Tonta
A
A
Breviario
F
F
Giavazzi
R
R
D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects*; D002460:Cell Line; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000648:ultrastructure; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology*; D007700:Kinetics; D051379:Mice; D008819:Mice, Nude; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000473:pathology* / Q000648:ultrastructure; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D007375:Interleukin-1; D014409:Tumor Necrosis Factor-alpha