title: Stimulation of DNA synthesis in cultured primary human mesothelial cells by specific growth factors.
abstract: Monolayer cultures of human mesothelial cells made quiescent by serum deprivation are induced to undergo one round of DNA synthesis by platelet-derived growth factor (PDGF), epidermal growth factor (EGF), or transforming growth factor type beta 1 (TGF-beta 1). This one-time stimulation is independent of other serum components. The kinetics for induction of DNA synthesis observed for PDGF, EGF, and TGF-beta 1 are all similar to one another, with a peak of DNA synthesis occurring 24-36 h after the addition of the growth factors. Repetitive rounds of DNA synthesis and cell division do not ensue after addition of PDGF, EGF, or TGF-beta 1 alone or in combination; however, in media supplemented with chemically denatured serum, each of these factors is capable of sustaining continuous replication of mesothelial cells. Stimulation of growth by PDGF and TGF-beta 1 is unusual for an epithelial cell type, and indicates that mesothelial cells have growth regulatory properties similar to connective tissue cells.
authors.affiliation: Laboratory of Human Carcinogenesis, National Cancer Institute, Bethesda, MD 20892.
authors.forename: E W
authors.initials: EW
authors.lastname: Gabrielson
authors.forename: B I
authors.initials: BI
authors.lastname: Gerwin
authors.forename: C C
authors.initials: CC
authors.lastname: Harris
authors.forename: A B
authors.initials: AB
authors.lastname: Roberts
authors.forename: M B
authors.initials: MB
authors.lastname: Sporn
authors.forename: J F
authors.initials: JF
authors.lastname: Lechner
mesh_terms: D002478:Cells, Cultured; D004261:DNA Replication / Q000187:drug effects*; D004815:Epidermal Growth Factor / Q000494:pharmacology; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism; D006133:Growth Substances / Q000494:pharmacology*; D006801:Humans; D007700:Kinetics; D010455:Peptides / Q000494:pharmacology; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D013936:Thymidine / Q000378:metabolism; D015291:Transforming Growth Factors; D014316:Tritium
chemical_list: D006133:Growth Substances; D010455:Peptides; D010982:Platelet-Derived Growth Factor; D014316:Tritium; D004815:Epidermal Growth Factor; D015291:Transforming Growth Factors; D013936:Thymidine