title: Isolation of protective T cells from BALB/cJ mice chronically infected with Leishmania donovani.
abstract: BALB/cJ mice, which are homozygous for Lshs on chromosone 1, are genetically susceptible to Leishmania donovani (S3), but spontaneously reduce their parasite burdens late in the course of infection. Spleens from chronically infected mice (5 to 8 mo) were found to have T cells that responded to leishmanial Ag by proliferating and secreting immune IFN. An IFN-secreting T cell line derived from the spleen of a chronically infected mouse, after boosting with leishmanial Ag and treatment with Pentostam to kill residual parasites, was able to activate macrophages to kill amastigotes in vitro. Furthermore, after adoptive transfer of this cell line, naive BALB/cJ mice challenged with amastigotes demonstrated a 42-fold reduction in parasite burden compared to controls. Four of five clones derived from the protective T cell line secreted IFN and proliferated between days 7 and 14 after Ag stimulation. All clones were Ly1+2-, L3T4+. Another T cell line, which had Ly1+2-, L3T4+ phenotype, was derived from the lymph nodes of s.c. immunized, uninfected mice. It multipled but never produced IFN in response to leishmanial Ag and failed to protect macrophages against L. donovani infection in vitro or in vivo. This nonprotective T cell line and the fifth clone from the protective line, which also did not secrete IFN, proliferated between days 2 and 7 after Ag stimulation. In summary, leishmania-responsive helper/inducer T cells, which produced IFN but were slow to proliferate in response to Ag in vitro and which were able to activate macrophages to reduce amastigotes in vitro and in vivo, are present in chronically infected BALB/cJ mice and may mediate the decrease in parasite burden during chronic infection.
authors.affiliation: Department of Microbiology, University of Virginia School of Medicine, Charlottesville 22908.
authors.forename: B
authors.initials: B
authors.lastname: Holaday
authors.forename: M D
authors.initials: MD
authors.lastname: Sadick
authors.forename: R D
authors.initials: RD
authors.lastname: Pearson
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000008:administration & dosage; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D002908:Chronic Disease; D002999:Clone Cells / Q000145:classification; D006224:Cricetinae; D007113:Immunity, Innate; D007893:Leishmania donovani / Q000254:growth & development / Q000276:immunology; D007898:Leishmaniasis, Visceral / Q000276:immunology* / Q000469:parasitology / Q000517:prevention & control; D008297:Male; D008647:Mesocricetus; D051379:Mice; D008807:Mice, Inbred BALB C; D010641:Phenotype; D013154:Spleen / Q000473:pathology; D013601:T-Lymphocytes / Q000145:classification / Q000276:immunology* / Q000469:parasitology
chemical_list: D000953:Antigens, Protozoan