Inactivation of the alpha-actinin gene in Dictyostelium.
alpha-Actinin-negative transformants of Dictyostelium have been obtained by transforming cells with a transformation vector carrying part of the alpha-actinin gene in either sense or antisense orientation. The transformants did not produce detectable alpha-actinin anymore and contained an altered RNA lacking the 3' part of the coding sequences. The deficiency in alpha-actinin was due to an integration of the transformation vector into the gene, since it could be detected by Southern blot analysis in the endogenous gene.
Noegel
A A
AA
Max-Planck-Institut fuer Biochemie, Martinsried, Federal Republic of Germany.
Witke
W
W
D000185:Actinin / Q000235:genetics*; D015152:Blotting, Northern; D015139:Blotting, Southern; D004023:Dictyostelium / Q000235:genetics*; D005786:Gene Expression Regulation; D005796:Genes; D005800:Genes, Fungal; D005822:Genetic Vectors; D009154:Mutation; D014170:Transformation, Genetic
D000185:Actinin