Natural cytotoxic cell-specific cytotoxic factor produced by IL-3-dependent basophilic/mast cells. Relationship to TNF.
The murine IL-3-dependent mast cell line, PT18-A17, and the rat basophilic leukemia cell line, RBL-2H3, were found to mediate natural cytotoxic (NC) activity via the release of a soluble factor which specifically lysed NC-sensitive WEHI-164 but not NK-sensitive YAC-1 tumor cells. The release of this NC cell-specific cytotoxic factor was enhanced by triggering of both types of cells via IgE receptor bridging. This factor had activity on TNF-sensitive but not TNF-resistant cell lines and could be neutralized by two independently produced polyclonal anti-mouse TNF antisera. It was not neutralized by antibodies against mouse IFN-alpha/beta or IFN-gamma. Moreover, it was not neutralized by a monoclonal or a polyclonal anti-human TNF, demonstrating that the rodent TNF differed antigenically from human TNF. These results indicate that the cytotoxic factor released from a murine IL-3-dependent mast cell line and from a rat basophilic leukemia cell line is immunologically and functionally related to murine TNF.
Richards
A L
AL
Department of Medical Microbiology/Immunology, University of South Florida, Tampa 33612.
Okuno
T
T
Takagaki
Y
Y
Djeu
J Y
JY
D000818:Animals; D001491:Basophils / Q000276:immunology / Q000378:metabolism*; D002460:Cell Line; D002474:Cell-Free System; D003602:Cytotoxicity, Immunologic; D007377:Interleukin-3; D007694:Killer Cells, Natural / Q000276:immunology*; D055646:Killer Factors, Yeast; D008407:Mast Cells / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D009500:Neutralization Tests; D014176:Protein Biosynthesis; D011506:Proteins / Q000276:immunology / Q000502:physiology; D051381:Rats; D014409:Tumor Necrosis Factor-alpha / Q000502:physiology*
D007377:Interleukin-3; D055646:Killer Factors, Yeast; D011506:Proteins; D014409:Tumor Necrosis Factor-alpha