Stepwise precipitation method of human blood lipoproteins for clinical routine.
A three-step precipitation method for separate measurement of the lipid and protein composition of the main serum lipoprotein classes has been developed. The consecutive procedure applying phosphotungstic acid, polyethylene glycol and dextran sulfate makes the analysis of chylomicron, VLDL, LDL, HDL2 and HDL3 possible. Because of simplicity and appropriate accuracy the method may be suitable for systematical use in clinical laboratories. A relatively large scale investigation on hyperlipidemic patients by clinics disposing of regular service could be managed with the aid of the method. In this study the lipoprotein composition of a limited number of normal, type IIA and type IV hyperlipidemic patients has been investigated. The results were compared to the international standard values.
Ecsedi
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GG
Research Centre of Chinoin Pharmaceutical and Chemical Works, Ltd., Budapest, Hungary.
Virág
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S
D000328:Adult; D001055:Apolipoproteins B / Q000097:blood; D005260:Female; D006801:Humans; D007202:Indicators and Reagents; D008055:Lipids / Q000097:blood; D008074:Lipoproteins / Q000097:blood* / Q000302:isolation & purification; D008297:Male; D008875:Middle Aged
D001055:Apolipoproteins B; D007202:Indicators and Reagents; D008055:Lipids; D008074:Lipoproteins