Polyoma antigen driven T lymphocytes with antitumor and DTH activity in CBA mice.
T lymphocytes isolated from a bulk culture of splenocytes exposed in vitro to polyoma TAA and IL-2, taken from CBA mice with transplanted polyoma tumors, exhibited both antitumor and DTH activity to polyoma TAA, associated with the Lyt 1+ phenotype, and also showed an increased affinity to TAA. The antitumor efficacy of adoptive immunotherapy, consisting in the i.p. administration of these TAA-driven lymphocytes, showed time and IL-2 dependence, while transferred cells were capable of eliciting DTH independently of IL-2. The suppression of tumor growth observed in 70% of the animals in the optimal variant seems to result from the cooperation of transferred lymphocytes with the recipients own lymphocytes.
Ochałek
T
T
Department of Microbiology and Immunology, Jan Zurzycki Institute of Molecular Biology, Jagiellonian University, Kraków, Poland.
Cadel
Z
Z
D000818:Animals; D000951:Antigens, Neoplasm / Q000276:immunology*; D005260:Female; D006968:Hypersensitivity, Delayed / Q000276:immunology*; D007116:Immunization, Passive; D007376:Interleukin-2 / Q000276:immunology; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D009368:Neoplasm Transplantation; D013601:T-Lymphocytes / Q000276:immunology* / Q000637:transplantation; D014412:Tumor Virus Infections / Q000276:immunology* / Q000473:pathology / Q000628:therapy
D000951:Antigens, Neoplasm; D007376:Interleukin-2