Detection of tetrodotoxin by thin-layer chromatography/fast atom bombardment mass spectrometry.
A new method for detection of tetrodotoxin (TTX) by thin-layer chromatography/fast atom bombardment (FAB) mass spectrometry was developed. TTX and/or related substances were separated by TLC on LHP-K high-performance precoated plates, with a solvent system of pyridine:ethyl acetate:acetic acid:water (15:5:3:4). The plates were subjected to positive FAB mass spectrometry, under scanning within a mass range from m/z 100 to 500. TTX was identified by selected ion-monitored chromatograms at m/z 320 (M + H)+ and 302 (M + H - H2O)+, along with full scan positive ion FAB mass spectrometry. The limit of detection for TTX was about 0.1 micrograms. TTX was also detected by cellulose acetate membrane electrophoresis/FAB mass spectrometry.
Nagashima
Y
Y
Department of Food Science and Technology, Tokyo University of Fisheries, Japan.
Nishio
S
S
Noguchi
T
T
Arakawa
O
O
Kanoh
S
S
Hashimoto
K
K
D000818:Animals; D003386:Brachyura / Q000032:analysis; D002855:Chromatography, Thin Layer / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D011799:Quinazolines / Q000032:analysis; D013779:Tetrodotoxin / Q000031:analogs & derivatives / Q000032:analysis*
D011799:Quinazolines; C047739:anhydrotetrodotoxin; C059207:tetrodonic acid; D013779:Tetrodotoxin