High-performance liquid chromatographic determination of a naturally occurring xylosyl thioether, 9-[5'-deoxy-5'(methylthio)-beta-D-xylofuranosyl]adenine.
A high-performance liquid chromatographic (HPLC) method for the separation and purification of 9-[5'-deoxy-5'(methylthio)-beta-D-xylofuranosyl]adenine (xylosyl-MTA), a naturally occurring analogue of 5'-deoxy-5'-methylthioadenosine (MTA), has been developed. The compound was purified from perchloric extracts of biological samples by reversed-phase HPLC on an Ultrasil ODS RP-18 column. Isocratic elution resulted in a complete separation between the xylosyl thioether and MTA. The determination of xylosyl-MTA in the nudibranch mollusc Doris verrucosa was also performed. Its concentration ranged from 330 to 380 nmol/g, considerably exceeding the cellular levels of MTA.
Porcelli
M
M
Institute of Biochemistry of Macromolecules, I Medical School, University of Naples, Italy.
Cacciapuoti
G
G
Oliva
A
A
Zappia
V
V
D000243:Adenosine Deaminase / Q000378:metabolism; D000818:Animals; D001230:Aspergillus / Q000302:isolation & purification / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D003839:Deoxyadenosines / Q000031:analogs & derivatives* / Q000302:isolation & purification; D008974:Mollusca / Q000378:metabolism; D013056:Spectrophotometry, Ultraviolet
D003839:Deoxyadenosines; C056833:9-(5'-deoxy-5'-(methylthio)-xylofuranosyl)adenine; D000243:Adenosine Deaminase