A tissue-specific DNase I-hypersensitive site in a class II A alpha gene is under trans-regulatory control.
Class II major histocompatibility complex molecules are integral membrane glycoproteins whose distribution is limited to certain tissues. To identify the molecular basis for such specificity, the chromatin configuration of the class II A alpha gene was examined in intact nuclei from various cell types. We show that there are three DNase I-hypersensitive sites in the A alpha gene. One of these sites, located near the promoter region, is specific to cells that normally express class II molecules at some stage of differentiation. Furthermore, this tissue-specific site appears to be under trans-regulatory control.
Liou
H C
HC
Department of Cancer Biology and Medicine, Harvard School of Public Health, Boston, MA 02115.
Polla
B S
BS
Aragnol
D
D
Leserman
L D
LD
Griffith
I J
IJ
Glimcher
L H
LH
D000818:Animals; D001402:B-Lymphocytes / Q000502:physiology*; D001483:Base Sequence; D003850:Deoxyribonuclease I / Q000378:metabolism; D005786:Gene Expression Regulation; D000949:Histocompatibility Antigens Class II / Q000235:genetics*; D008562:Membrane Glycoproteins / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D013601:T-Lymphocytes / Q000502:physiology; D014157:Transcription Factors / Q000235:genetics
D000949:Histocompatibility Antigens Class II; D008562:Membrane Glycoproteins; D014157:Transcription Factors; D003850:Deoxyribonuclease I