Determination of circulating immune complexes by chicken anti-human C3 and anti-human C1q microELISA.
The present study reports on an anti-human C1q and an anti-human C3 microELISA for measuring circulating immune complexes (CIC). Affinity-purified chicken anti-human C3 and anti-human C1q were used as capture antibodies and protein A-alkaline phosphatase conjugate for detection. Chicken antibodies do not activate mammalian complement, do not react with rheumatoid factor and are not bound to anti-mammalian IgG antibodies or protein A, which often are used for detection in ELISA. They are therefore suitable as capture antibodies in CIC assays. We have tested Bell's palsy patients and found an increase in both anti-C3- and anti-C1q-containing CIC in acute and convalescent sera compared with the normal.
Larsson
A
A
Department of Medical and Physiological Chemistry, Biomedical Centre, Uppsala, Sweden.
Jonsson
L
L
Sjöquist
J
J
D000818:Animals; D000936:Antigen-Antibody Complex / Q000032:analysis*; D001667:Binding, Competitive; D001769:Blood / Q000276:immunology; D002645:Chickens; D003166:Complement Activating Enzymes / Q000276:immunology*; D003172:Complement C1 / Q000276:immunology*; D015922:Complement C1q; D003176:Complement C3 / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005158:Facial Paralysis / Q000276:immunology; D006801:Humans; D007106:Immune Sera; D007074:Immunoglobulin G; D046911:Macromolecular Substances; D008832:Microchemistry; D010949:Plasma / Q000276:immunology; D012710:Serum Albumin, Bovine / Q000276:immunology
D000936:Antigen-Antibody Complex; D003172:Complement C1; D003176:Complement C3; D007106:Immune Sera; D007074:Immunoglobulin G; D046911:Macromolecular Substances; D012710:Serum Albumin, Bovine; D015922:Complement C1q; D003166:Complement Activating Enzymes