Fast protein liquid chromatography of botulinum neurotoxin types A, B and E.
Three antigenically different botulinum neurotoxins (NTs, relative molecular mass approximately 150,000), classically distinguished only by specific antisera, were for the first time chromatographically resolved. Mixed NTs eluted from a Mono-Q column in order of types E, A and B, and from Mono-S as B, E and A. Type A and B NTs were successfully chromatographed on the cation-exchange Mono-S column above their isoelectric points. Purification of type A and B NTs by automated liquid chromatography was also accomplished for the first time. Type A, B and E NTs were purified by application on an anion-exchange Mono-Q column, followed by use of a cation-exchange Mono-S column.
Woody
M A
MA
Food Research Institute, University of Wisconsin, Madison 53706.
DasGupta
B R
BR
D001905:Botulinum Toxins / Q000032:analysis*; D002021:Buffers; D002850:Chromatography, Gel; D002853:Chromatography, Liquid; D004591:Electrophoresis, Polyacrylamide Gel
D002021:Buffers; D001905:Botulinum Toxins