Colorimetric determination of neutral sugars by a resorcinol sulfuric acid micromethod.
A microscale colorimetric assay for neutral sugars, in which neutral sugars react with resorcinol in the presence of 75% sulfuric acid solution is described. This assay, based on the use of microtiter plates with 96 U-shaped wells is simple and easy to handle; it allows accurate determinations with small samples (20 microliters) containing 1 to 100 nmol of neutral sugars and is quite convenient for detection of glycoconjugates in chromatographic column effluents.
Monsigny
M
M
Laboratoire de Biochimie Cellulaire et Moléculaire des Glycoconjugués, Centre National de la Recherche Scientifique, Orleans, France.
Petit
C
C
Roche
A C
AC
D002241:Carbohydrates / Q000032:analysis*; D003124:Colorimetry / Q000379:methods; D006001:Glycoconjugates / Q000032:analysis; D006601:Hexoses / Q000032:analysis; D007202:Indicators and Reagents; D008358:Mannose / Q000032:analysis; D008832:Microchemistry; D012118:Resorcinols; D013464:Sulfuric Acids
D002241:Carbohydrates; D006001:Glycoconjugates; D006601:Hexoses; D007202:Indicators and Reagents; D012118:Resorcinols; D013464:Sulfuric Acids; D008358:Mannose; C031389:resorcinol