Selection of high-performance liquid chromatographic methods in pharmaceutical analysis. I. Optimization for selectivity in reversed-phase chromatography.
The application of solvent optimization to the development of isocratic reversed-phase liquid chromatography has been reported in several publications. Two different approaches to solvent optimization for controlling band spacing for the maximum resolution of samples are "solvent strength" and "solvent type" optimization. To improve the separation selectivity further the combination of these two approaches was examined, as a (global) optimum mobile phase composition requires the optimization of the solvent strength by varying the percentage of organic component and of the solvent selectivity of methanol, acetonitrile, tetrahydrofuran and water. It was found that the combination of "solvent strength" and "solvent type" optimization provides a markedly better separation than either procedure alone.
Gazdag
M
M
Chemical Works of Gedeon Richter, Ltd., Budapest, Hungary.
Szepesi
G
G
Szeleczki
E
E
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004970:Estrone / Q000032:analysis; D004997:Ethinyl Estradiol / Q000032:analysis; D008656:Mestranol / Q000032:analysis; D009640:Norethindrone / Q000032:analysis; D009644:Norgestrel / Q000032:analysis; D013256:Steroids / Q000032:analysis*
D013256:Steroids; D004970:Estrone; D009644:Norgestrel; D004997:Ethinyl Estradiol; D008656:Mestranol; D009640:Norethindrone