Enzymes of the de novo and salvage pathways of purine synthesis in renal hypertrophy. Contrasting effects of early diabetes and unilateral nephrectomy.
Evidence for differences in the mechanism of renal growth in experimental diabetes and compensatory hypertrophy after unilateral nephrectomy (UN) has been obtained from measurements of the activity of enzymes of the de novo and salvage pathways of purine synthesis in the kidneys of diabetic and UN rats and in doubly lesioned animals. In diabetes, the activity of enzymes of both pathways increased. No effect of UN on the activity of any of these enzymes was observed, nor was the effect of the double lesion greater than the effect of diabetes alone. The activity of the pentose phosphate pathway increased in diabetes but not as a result of UN. Again, the effect of the double lesion was no different from that of diabetes alone. These results indicate that the accretion of nucleic acids in diabetes involves a larger component of de novo synthesis, contrasting with UN, where depressed breakdown may play a significant role.
Beardsley
S J
SJ
Courtauld Institute of Biochemistry, Middlesex Hospital Medical School, London, UK.
Kunjara
S
S
Sochor
M
M
Greenbaum
A L
AL
D000228:Adenine Phosphoribosyltransferase / Q000378:metabolism*; D000582:Amidophosphoribosyltransferase / Q000378:metabolism; D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000201:enzymology*; D003928:Diabetic Nephropathies / Q000201:enzymology; D006984:Hypertrophy; D007041:Hypoxanthine Phosphoribosyltransferase / Q000378:metabolism*; D007668:Kidney / Q000201:enzymology* / Q000473:pathology; D009392:Nephrectomy; D010430:Pentosyltransferases / Q000378:metabolism*; D011687:Purines / Q000096:biosynthesis*; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values
D011687:Purines; D010430:Pentosyltransferases; D000582:Amidophosphoribosyltransferase; D000228:Adenine Phosphoribosyltransferase; D007041:Hypoxanthine Phosphoribosyltransferase