Simultaneous analysis of methionine- and leucine-enkephalin from rat brain: quantification by liquid chromatography-electrochemistry.
This study focuses on the application of liquid chromatography with electrochemical detection (LC-ED) for the analysis of methionine-enkephalin (ME) and leucine-enkephalin (LE) extracted from rat brain regions. The high applied potentials necessary for enkephalin detection required the development of an efficient sample processing protocol. Brain extracts were processed using chromatographic mode sequencing (CMS). The decrease in electroactive interfering substances by CMS improved the chromatographic resolution of ME and LE and the electrode performance. Other qualitative and analytical methods were used to evaluate the enkephalin data obtained by LC-ED for rat brain regions. This study demonstrates that LC-ED provides both the sensitivity and specificity necessary for the analysis of enkephalins from rat brain regions.
Fleming
L H
LH
David I.M. Fine Memorial Neurology Research Laboratory, Department of Neurology University of Wisconsin Medical School, Sinai Samaritan Medical Center, Milwaukee 53201.
Reynolds
N C
NC
D000818:Animals; D001923:Brain Chemistry; D002853:Chromatography, Liquid; D004563:Electrochemistry; D004743:Enkephalin, Leucine / Q000032:analysis*; D004744:Enkephalin, Methionine / Q000032:analysis*; D007202:Indicators and Reagents; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D007202:Indicators and Reagents; D004744:Enkephalin, Methionine; D004743:Enkephalin, Leucine