title: A novel method for nucleic acid sequence determination.
abstract: We describe a novel sequencing methodology which should be readily and completely automated. The method relies on fragmentation of a nucleotide or deoxynucleotide sequence into short fragments, and subsequent quantitation of the fragments by hybridization to oligo-deoxynucleotides on a solid support. The original sequence may be reconstructed from the resulting table of fragment frequencies. We present a specific protocol which would allow practical implementation of this approach.
authors.affiliation: Department of Biochemistry, University of Bath, U.K.
authors.forename: W
authors.initials: W
authors.lastname: Bains
authors.forename: G C
authors.initials: GC
authors.lastname: Smith
mesh_terms: D001483:Base Sequence; D008722:Methods; D009693:Nucleic Acid Hybridization; D012091:Repetitive Sequences, Nucleic Acid