Modulation of the immune response by proopiomelanocortin derived peptides. II. Influence of adrenocorticotropic hormone on the rise in intracellular free calcium concentration after T cell activation.
In this paper the effect of ACTH 1-24 is described on one of the "early events" occurring during T cell activation: the rise in the concentration of cytosolic free calcium. The increase in cytosolic free calcium induced by concanavalin A or by a monoclonal antibody with specificity for the T cell receptor-T3 complex could be modulated by physiological concentrations of ACTH 1-24. The modulation of the response appeared to be dose dependent. The effect of ACTH 1-24 was maximal at 10(-10) M. No effect of ACTH 1-24 was observed on the basal level of cytosolic free calcium. These results indicate that physiological doses of ACTH can interfere with the responsiveness of T cells to mitogenic and antigenic stimuli.
Kavelaars
A
A
Department of Pediatric Immunology, University Hospital for Children and Youth Het Wilhelmina Kinderziekenhuis, Utrecht, The Netherlands.
Ballieux
R E
RE
Heijnen
C
C
D000911:Antibodies, Monoclonal / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D003208:Concanavalin A / Q000494:pharmacology; D003366:Cosyntropin / Q000494:pharmacology*; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D008213:Lymphocyte Activation / Q000187:drug effects*; D013601:T-Lymphocytes / Q000187:drug effects / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D003208:Concanavalin A; D003366:Cosyntropin; D002118:Calcium