title: Biosynthesis of 117 antigen: a cell cohesion molecule in Dictyostelium discoideum.
abstract: 117 antigen is involved in the process of intercellular cohesion in Dictyostelium discoideum [Brodie et al., 1983]. The antigen, a 69- and 72-kDa doublet, was found to arise from a 60- and 62-kDa precursor. The mature antigen contains N-linked oligosaccharides that are sulfated and fucosylated [Sadeghi et al., 1987]. These oligosaccharide chains are resistant to endoglycosidase H digestion. 117 antigen also contains a post-translationally added carbohydrate-containing modification(s). Unlike the N-linked oligosaccharide, this carbohydrate moiety is sensitive to periodate oxidation. 117 antigen is developmentally regulated, and the changes in rate of 117 antigen synthesis reflect changes in the cellular levels of its mRNA. 117 mRNA accumulates in starving cells and reaches its maximum when cells become aggregation competent. The mRNA levels then decline, and by the time the slug structure is formed, no 117 mRNA is present. 117 mRNA reaccumulates for a brief period during early culmination and then returns to an undetectable level.
authors.affiliation: E.A. Doisy Department of Biochemistry, St. Louis University Medical School, MO 63104.
authors.forename: H
authors.initials: H
authors.lastname: Sadeghi
authors.forename: A
authors.initials: A
authors.lastname: da Silva
authors.forename: C
authors.initials: C
authors.lastname: Klein
mesh_terms: D000946:Antigens, Fungal / Q000096:biosynthesis* / Q000235:genetics; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules; D004023:Dictyostelium / Q000254:growth & development* / Q000502:physiology; D007700:Kinetics; D012333:RNA, Messenger / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D000946:Antigens, Fungal; D015815:Cell Adhesion Molecules; D012333:RNA, Messenger; C054291:antigen 117, Dictyostelium