Thin-layer chromatographic study of the metabolites of erythromycins in the Wistar rat.
The metabolites of erythromycin A, anhydroerythromycin A, N-demethylerythromycin A and erythromycin B in the Wistar rat were studied by thin-layer chromatography. In some experiments germ-free rats, rats with a cannulated bile duct and a gastrectomized rat were used. The erythromycins examined were shown to undergo two principal changes, N-demethylation and acid-catalysed degradation. It was demonstrated that the stomach and the liver are not the sole sites of acid degradation and demethylation of erythromycins, respectively. Erythromycin A gives three principal metabolites, anhydroerythromycin A, anhydro-N-demethylerythromycin A and N-demethylerythromycin A, and erythromycin A enol ether and N-demethylerythromycin A enol ether are present to a minor extent. 5-O-Desosaminylerythronolide A was also identified, suggesting the presence of an erythromycin glycosidase.
Kibwage
I O
IO
Katholieke Universiteit Leuven, Instituut voor Farmaceutische Wetenschappen, Belgium.
Roets
E
E
Verbruggen
A
A
Hoogmartens
J
J
Vanderhaeghe
H
H
D000818:Animals; D001646:Bile / Q000032:analysis; D055598:Chemical Phenomena; D002621:Chemistry; D002855:Chromatography, Thin Layer; D004917:Erythromycin / Q000031:analogs & derivatives / Q000378:metabolism* / Q000652:urine; D005243:Feces / Q000032:analysis; D005260:Female; D008297:Male; D013058:Mass Spectrometry; D051381:Rats; D011919:Rats, Inbred Strains; D014018:Tissue Distribution
D004917:Erythromycin