title: Effects of system peaks in ion-pair reversed-phase liquid chromatography for noscapine and metabolites.
abstract: System peaks were generated in an ion-pair reversed-phase system by co-injection of an alkylsulphate with the analytes. The acidic mobile phase contained acetonitrile and an aliphatic tertiary amine as a co-ion. The retention time of the system peak was regulated by the concentration and hydrophobicity of the co-ion and the alkylsulphate. The peak performance of the analytes was affected by co-elution with a system peak. Both peak distortions and improvements appeared, and the principles for the latter could be applied in a dual-column system involving trace enrichment and column switching.
authors.affiliation: Department of Research and Development, ACO Läkemedel AB, Solna, Sweden.
authors.forename: M
authors.initials: M
authors.lastname: Johansson
authors.forename: D
authors.initials: D
authors.lastname: Westerlund
mesh_terms: D000588:Amines / Q000032:analysis; D002853:Chromatography, Liquid; D013058:Mass Spectrometry; D009665:Noscapine / Q000032:analysis* / Q000378:metabolism; D013056:Spectrophotometry, Ultraviolet; D013431:Sulfates / Q000032:analysis
chemical_list: D000588:Amines; D013431:Sulfates; D009665:Noscapine