Suppression of liver regeneration resulting from intravenous injection of splenic glass adherent cells activated by poly I:C.
Syngeneic spleen cells (SPCs) were treated with poly I:C in the presence of DEAE-dextran for 2 h in vitro for their activation (IC-SPCs). When these IC-SPCs were inoculated into 70% partially hepatectomized mice, liver regeneration of the mice was strongly suppressed. The extent of suppression was dependent upon the number of injected cells and upon the concentration of poly I:C for in vitro treatment. Among IC-SPCs, the suppressive activity was predominantly caused by splenic glass adherent cells pretreated with poly I:C (IC-SACs) than by pretreated splenic T cells (IC-T). IC-SACs additionally cultured without poly I:C for 24 h lost their suppressive activity; however, when they were cultured in the presence of indomethacin, they could retain their activity, and interleukin 1 (IL 1) activity in culture supernatant was greater in the group cultured with indomethacin than without it. These results suggest that the suppressive activity of IC-SACs was not solely caused by the poly I:C carried into the mice with IC-SACs but by the activated IC-SACs themselves. The peak of liver cell mitosis in mice injected with IC-SACs was delayed 24 h as compared with non-injected groups, but IC-SACs-injected mice did not lose their regenerative potential. These findings demonstrate that the activated immune system may have some regulatory effects on liver regeneration.
Takahashi
H
H
Department of Microbiology and Immunology, Nippon Medical School, Tokyo, Japan.
Takeshita
T
T
Yokomuro
K
K
D000818:Animals; D005260:Female; D007107:Immune System / Q000502:physiology; D066298:In Vitro Techniques; D007375:Interleukin-1 / Q000096:biosynthesis; D008115:Liver Regeneration; D051379:Mice; D008807:Mice, Inbred BALB C; D011070:Poly I-C / Q000494:pharmacology*; D013154:Spleen / Q000276:immunology*
D007375:Interleukin-1; D011070:Poly I-C