title: Purification of 6-phosphogluconolactonase from bass (Dicentrarchus labrax L.)liver.
abstract: The enzyme 6-phosphogluconolactonase (EC 3.1.1.31) is present at high levels in bass liver. The enzyme has been purified 1253-fold with an overall yield of 78% in a procedure involving dye-ligand and gel filtration chromatographies. The purified enzyme which is homogenous by all the usual criteria has a molecular weight of about 30,000. It exhibits a considerably high catalytic efficiency with Kcat/Km of 8.5 x 10(7) M-1.s-1 at 22 degrees C. Its activity illustrates the importance of glucose oxidation via the pentose phosphate pathway relative to glycolysis in this organism.
authors.affiliation: Departamento de Bioquímica. Facultad de Veterinaria. Universidad Complutense, Madrid, Spain.
authors.forename: M M
authors.initials: MM
authors.lastname: Medina-Puerta
authors.forename: M
authors.initials: M
authors.lastname: Gallego-Iniesta
authors.forename: A
authors.initials: A
authors.lastname: Garrido-Pertierra
mesh_terms: D000818:Animals; D001492:Bass / Q000378:metabolism*; D002265:Carboxylic Ester Hydrolases / Q000032:analysis / Q000302:isolation & purification*; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006868:Hydrolysis; D008099:Liver / Q000201:enzymology*; D010473:Perciformes / Q000378:metabolism*; D011506:Proteins / Q000032:analysis
chemical_list: D011506:Proteins; D002265:Carboxylic Ester Hydrolases; C022363:6-phosphogluconolactonase