Do pyrimidine nucleotides regulate translatability of globin mRNA as purine nucleotides do?
1. When rabbit globin mRNA was incubated with rabbit reticulocyte lysate in the presence of various concentrations of nucleotides, globin synthesis was inhibited or stimulated dependent on dose. 2. Pyrimidine nucleotides inhibited protein synthesis at 0.3 mM, whereas 2 mM of purine nucleotides were required to cause similar inhibition. 3. Adenosine mono- and diphosphate inhibited globin synthesis at a concentration of only 1 mM; however, the sequence is AMP greater than ADP greater than ATP. 4. Translation arrest by these nucleotides was instantaneous. 5. These results suggest that these nucleotides may provide a structural component for maintaining the integrity, the conformation of mRNA or of the messenger ribonucleoprotein (mRNP).
Rubin
H N
HN
Almendarez
E
E
Halim
M N
MN
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000249:Adenosine Monophosphate / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D002850:Chromatography, Gel; D003570:Cytidine Triphosphate / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D005914:Globins / Q000096:biosynthesis / Q000235:genetics*; D006160:Guanosine Triphosphate / Q000494:pharmacology; D011061:Poly A / Q000378:metabolism; D014176:Protein Biosynthesis / Q000187:drug effects*; D011685:Purine Nucleotides / Q000494:pharmacology*; D011742:Pyrimidine Nucleotides / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism*; D011817:Rabbits; D014544:Uridine Triphosphate / Q000494:pharmacology
D011685:Purine Nucleotides; D011742:Pyrimidine Nucleotides; D012333:RNA, Messenger; D011061:Poly A; D000249:Adenosine Monophosphate; D000244:Adenosine Diphosphate; D003570:Cytidine Triphosphate; D006160:Guanosine Triphosphate; D000255:Adenosine Triphosphate; D005914:Globins; D014544:Uridine Triphosphate