In vitro modulation of human monocytes functions by RU 41740 (Biostim).
The in vitro effects of RU 41740, a mixture of two glycoproteins extracted from K. pneumoniae and possessing immunomodulatory activity, on human monocyte's cytotoxic activity, migratory capacity and monokine production were investigated. Exposure to RU 41740 in nanogram concentrations was associated with rapid increases in IL-1 and TNF production and marked augmentation in the monocytes' killing of TNF-sensitive targets, whereas killing of TNF-insensitive targets required longer exposures to microgram concentrations of this drug. Using otherwise effective treatment conditions, RU 41740 did not directly affect monocyte's spontaneous and FMLP-induced migration. The possible role of mononuclear cells stimulation and increased production of effector and regulatory monokines in the immunomodulatory activity of this product is addressed.
Sozzani
S
S
Laboratory of Immunology and Oncology, Mario Negri Institute for Pharmacological Research, Milan, Italy.
D'Alessandro
F
F
Capsoni
F
F
Luini
W
W
Barcellini
W
W
Guidi
G
G
Spreafico
F
F
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000494:pharmacology*; D002465:Cell Movement; D003602:Cytotoxicity, Immunologic; D006801:Humans; D066298:In Vitro Techniques; D007375:Interleukin-1 / Q000096:biosynthesis; D009000:Monocytes / Q000276:immunology* / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D000276:Adjuvants, Immunologic; D001426:Bacterial Proteins; D007375:Interleukin-1; D014409:Tumor Necrosis Factor-alpha; C037567:glycoprotein, Klebsiella pneumoniae