title: [Synthesis and properties of DNA duplexes containing hydrocarbon bridges instead of a nucleoside residue].
abstract: DNA duplexes 14 bp long containing an EcoRII and MvaI restriction site in which a nucleoside is substituted by 1,3-diaminopropane or 1,3-propanediol residue have been chemically synthesized. Diaminopropane bridge was introduced by the chemical ligation, whereas the oligonucleotide containing propanediol was prepared by automatic solid phase phosphoroamidite method on "Victoria-4M" synthesizer. As CD and UV spectra show, the modification destabilises the duplex by 18-20 degrees C without essential distortion of the double helix, except for increase of the conformational mobility in the modified site.
authors.forename: S A
authors.initials: SA
authors.lastname: Kuznetsova
authors.forename: E M
authors.initials: EM
authors.lastname: Volkov
authors.forename: E S
authors.initials: ES
authors.lastname: Gromova
authors.forename: V K
authors.initials: VK
authors.lastname: Potapov
authors.forename: Z A
authors.initials: ZA
authors.lastname: Shabarova
mesh_terms: D001483:Base Sequence; D002942:Circular Dichroism; D004247:DNA / Q000138:chemical synthesis*; D003959:Diamines; D009690:Nucleic Acid Conformation; D009692:Nucleic Acid Heteroduplexes / Q000138:chemical synthesis*; D009705:Nucleosides; D009841:Oligonucleotides / Q000032:analysis; D011409:Propylene Glycols; D013056:Spectrophotometry, Ultraviolet
chemical_list: D003959:Diamines; D009692:Nucleic Acid Heteroduplexes; D009705:Nucleosides; D009841:Oligonucleotides; D011409:Propylene Glycols; C041787:1,3-propanediol; D004247:DNA; C009475:trimethylenediamine
vernacular_title: Sintez i svoĭstva DNK-dupleksov, soderzhashchikh uglevodorodnye mostiki vmesto odnogo iz nukleozidnykh ostatkov.