Isolation of protein kinase C subspecies from a preparation of human T lymphocytes.
Using a preparation of purified human T lymphocytes, we were able to resolve a partially purified protein kinase C (PKC) enzyme fraction into two distinct subspecies, of approximately equal activity. Biochemical and immunocytochemical analysis revealed that these fractions closely resembled the type II (beta) and type III(alpha) PKC subspecies previously identified and characterised from brain tissue. These results provide valuable information for further studies on the role of individual PKC subspecies in T lymphocyte proliferation.
Shearman
M S
MS
Department of Biochemistry, Kobe University School of Medicine, Japan.
Berry
N
N
Oda
T
T
Ase
K
K
Kikkawa
U
U
Nishizuka
Y
Y
D002122:Calcium Chloride / Q000494:pharmacology; D002852:Chromatography, Ion Exchange; D004075:Diglycerides / Q000494:pharmacology; D004789:Enzyme Activation; D006801:Humans; D007527:Isoenzymes / Q000302:isolation & purification* / Q000378:metabolism; D007700:Kinetics; D010718:Phosphatidylserines / Q000494:pharmacology; D011493:Protein Kinase C / Q000302:isolation & purification* / Q000378:metabolism; D013601:T-Lymphocytes / Q000201:enzymology*
D004075:Diglycerides; D007527:Isoenzymes; D010718:Phosphatidylserines; D011493:Protein Kinase C; D002122:Calcium Chloride; C013965:diolein