Primary structure of a ribonuclease from bovine brain.
The primary structure of a pyrimidine base-specific ribonuclease from bovine brain was determined. The sequence determined is (sequence; see text). Although the sequence homology of this RNase with bovine pancreatic RNase A is 78.2%, it consists of 140 amino acid residues, and it is 16 amino acid residues longer than RNase A at the carboxyl-terminal. In addition to an N-glycosylated long carbohydrate chain, the bovine brain RNase has two short O-glycosylated carbohydrate chains at the 129th and the 133rd serine residues. The additional C-terminal tail of the bovine brain RNase has a unique composition: 6 proline, 5 hydrophobic amino acids, and two basic amino acids, arginine and histidine.
Watanabe
H
H
Department of Microbiology, Hoshi College of Pharmacy, Tokyo.
Katoh
H
H
Ishii
M
M
Komoda
Y
Y
Sanda
A
A
Takizawa
Y
Y
Ohgi
K
K
Irie
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000201:enzymology*; D002417:Cattle; D002918:Chymotrypsin; D003488:Cyanogen Bromide; D008969:Molecular Sequence Data; D010179:Pancreas / Q000201:enzymology; D010446:Peptide Fragments / Q000302:isolation & purification; D012259:Ribonuclease, Pancreatic; D012260:Ribonucleases; D012697:Serine Endopeptidases
D010446:Peptide Fragments; D012260:Ribonucleases; D012259:Ribonuclease, Pancreatic; D012697:Serine Endopeptidases; D002918:Chymotrypsin; C036813:lysyl endopeptidase; D003488:Cyanogen Bromide