The age-related decline in interleukin-3 expression in mice.
Spleen lymphocytes form 5- to 37-month-old C57BL/6J mice were stimulated by concanavalin A (con A) in vitro, and the interleukin-3 (IL-3) expression was assessed by measuring the IL-3 activity in culture supernatants and the cytoplasmic IL-3 mRNA levels. The activity and mRNA level of IL-3 was maximum at 20 hr after culturing in the presence of con A. The IL-3 activity in the culture supernatants and the IL-3 mRNA level in lymphocytes declined 70% to 80% between 5 and 37 months of age. Northern blot analysis revealed no change in the size of IL-3 mRNA between young and old mice. When the expression of IL-3 and interleukin-2 (IL-2) by con A-stimulated lymphocytes was compared, both interleukins showed a similar declined with age.
Li
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DD
Department of Chemistry, Illinois State University, Normal 61761.
Chien
Y K
YK
Gu
M Z
MZ
Richardson
A
A
Cheung
H T
HT
D000375:Aging; D000818:Animals; D015152:Blotting, Northern; D003208:Concanavalin A / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects; D007376:Interleukin-2 / Q000235:genetics; D007377:Interleukin-3 / Q000235:genetics / Q000378:metabolism*; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000378:metabolism*; D051379:Mice; D012333:RNA, Messenger / Q000378:metabolism; D013154:Spleen / Q000166:cytology
D007376:Interleukin-2; D007377:Interleukin-3; D012333:RNA, Messenger; D003208:Concanavalin A