B cells expressing Ig transgenes respond to a T-dependent antigen only in the presence of Ia-compatible T cells.
We sought a quicker and easier method for the isolation of B cells enriched for a given Ag specificity. Here, we report our results using transgenic mice bearing mu- and kappa-transgenes that encode an IgR against the hapten phosphorylcholine. Splenic B cells from such mice had a high percentage of phosphorylcholine-binding cells and differentiated in vitro in response to T cell hybridomas in an Ag-specific, Ia-restricted manner. Supernatants from such in vitro cultures could not transfer helper activity, nor could the T cell be replaced in other ways tried when using a classical T-dependent Ag. These results support the model of a cognate interaction between T and B cell, and we present evidence that the interaction may consist of two or more steps.
O'Brien
R L
RL
Howard Hughes Medical Institute, Denver, CO 80206.
Marrack
P
P
Storb
U
U
Kappler
J W
JW
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D002352:Carrier Proteins / Q000276:immunology; D002450:Cell Communication; D002478:Cells, Cultured; D005803:Genes, Immunoglobulin; D006241:Haptens / Q000276:immunology*; D000949:Histocompatibility Antigens Class II / Q000235:genetics / Q000276:immunology*; D007700:Kinetics; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL / Q000235:genetics; D008822:Mice, Transgenic; D010767:Phosphorylcholine / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D002352:Carrier Proteins; D006241:Haptens; D000949:Histocompatibility Antigens Class II; D010767:Phosphorylcholine