[Role of the cytosolic factor in the interaction of [3H] alpha-tocopherol with rat liver nuclei].
A protein fraction (Mr = 30-70 kD) specifically binding [3H]alpha-tocopherol was isolated from rat liver cytosol. Using high performance ion exchange chromatography, this fraction was separated into acid and alkaline protein subfractions. Acid proteins make up to 41% of the total protein pool and they bind the label 8 times more intensively than the alkaline ones. Cytosol and its protein fraction with an average molecular mass increase 2.2-2.5-fold the binding of labeled vitamin E to isolated liver nuclei. It is concluded that the cytosolic proteins having a medium molecular mass are involved in tocopherol interaction with the nuclei.
Donchenko
G V
GV
Malen'kikh
L B
LB
Kapralov
A A
AA
Baryk
O Ia
OIa
Mel'nik
V N
VN
D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism*; D005260:Female; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D014810:Vitamin E / Q000378:metabolism / Q000494:pharmacology*
D002352:Carrier Proteins; D014810:Vitamin E
Uchastie tsitozol'nogo faktory vo vzaimodeĭstvii [3H]alpha-tokoferola s iadrami pecheni krys.