Simple LC method for determination of chloramphenicol in equine, canine, and feline serum.
A simple and sensitive high-performance liquid chromatographic (HPLC) method is developed for the determination of chloramphenicol (CAP) concentrations in equine, canine, and feline serum. Serum samples are diluted, then ultrafiltered using a microconcentrator with a 30,000 MW cutoff filter. CAP is separated and quantitated directly from the ultrafiltrate on a reversed-phase column using a buffered methanol mobile phase and a variable wavelength UV detector set at 278 nm. The recovery of CAP from 5 micrograms/mL spiked serum samples is determined using 10 serum samples from each of the three species. This method of sample preparation yields recoveries of 99.8%, 92.0%, and 88.6% with coefficients of variation of 1.2%, 1.3%, and 1.0% in equine, canine, and feline sera, respectively. Concentrations of CAP between 0.5 and 400 micrograms/mL as determined by peak area are found to have a linear relationship (correlation coefficient = 0.99997, n = 10), although amounts as low as 0.025 microgram/mL can be detected.
Tyczkowska
K
K
Clinical Pharmacology Unit, School of Veterinary Medicine, North Carolina State University, Raleigh 27606.
Hedeen
K M
KM
Aucoin
D P
DP
Aronson
A L
AL
D000818:Animals; D002415:Cats; D002701:Chloramphenicol / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D004285:Dogs; D004563:Electrochemistry; D006736:Horses; D007202:Indicators and Reagents; D010783:Photometry; D013056:Spectrophotometry, Ultraviolet
D007202:Indicators and Reagents; D002701:Chloramphenicol