Interleukin-1-induced alterations in proteoglycan metabolism and matrix assembly.
In cartilage, the large chondroitin sulfate proteoglycan exists as aggregates by interacting with link protein and hyaluronic acid. In diseases associated with cartilage degeneration, the proteoglycan does not aggregate because of a defect in the hyaluronate-binding activity. Since interleukin-1 (IL-1) is a secretory product of activated macrophages and may influence the cartilage function in joints, we studied the effects of IL-1 on the synthesis and assembly of proteoglycan by rabbit articular chondrocytes in culture. IL-1-treated cells showed a modest increase in the total proteoglycan synthesis, but also showed a more pronounced decrease in the incorporation of extracellular matrix. Affinity chromatography of the conditioned media on hyaluronic acid-Sepharose revealed that all of the proteoglycan of control cells strongly bound to hyaluronate. The IL-1-treated medium contained two fractions: one that was strongly bound to the column and a second that did not bind. The results demonstrate that the IL-1-treated cells cannot incorporate proteoglycan into the matrix partly because of a defect in the proteoglycan molecules and partly due to other mechanisms regulating proteoglycan assembly.
Chandrasekhar
S
S
Department of Connective Tissue Research, Lilly Research Laboratories, Indianapolis, Indiana 46285.
Phadke
K
K
D000818:Animals; D001665:Binding Sites / Q000187:drug effects; D002358:Cartilage, Articular / Q000378:metabolism*; D002478:Cells, Cultured; D002846:Chromatography, Affinity; D003470:Culture Media / Q000032:analysis; D005109:Extracellular Matrix / Q000378:metabolism*; D006820:Hyaluronic Acid / Q000378:metabolism; D007375:Interleukin-1 / Q000494:pharmacology*; D008262:Macrophage Activation; D008264:Macrophages; D011509:Proteoglycans / Q000032:analysis / Q000096:biosynthesis*; D011817:Rabbits
D003470:Culture Media; D007375:Interleukin-1; D011509:Proteoglycans; D006820:Hyaluronic Acid