Optimization of platelet concentrate collection for continuous flow cell separation devices (CFCS).
The ability to collect and transfuse a known quantity of platelets to a thrombocytopenic patient, which would assure hemostasis, requires that the quantity needed for transfusion is known, and that the quantity can be predictably collected from the donor. Collection of single donor platelet concentrates (SDPC) by continuous flow cell separation (CFCS) was introduced some ten years ago, but the variables that contribute to the yield have not been integrated into any mathematical model that could be implemented by the device to produce predictable yields, and automation alone has been insufficient to result in predictability. Three major factors contribute to platelet yield: the biologic contribution of the donor; procedure collection parameters, and the efficiency of the device. Data collected from Spectra, COBE 2997 and CS 3000 SDPC procedures suggested that the 35 x 10(11) average yield of PC represented 20-35% of the total quantity (TQ) available in the intravascular space of the donor. The percent collected from individual multiply pheresed donors, however, was consistent over multiple procedures. The volume of blood processed (a procedure variable) is also some percent of the blood volume (BV) of the donor. When these two variables (% TQ and % BV) were combined and statistical correlations to the yield examined, there were sufficiently consistent relationships between the two to mathematically model equations that predict reasonably well the yield on any of the CFCS devices. A light monitoring device attached to the Spectra translates changes in light transmission to platelet concentration in the collect line, and electronically tracks the accumulating yield throughout the procedure.(ABSTRACT TRUNCATED AT 250 WORDS)
Hester
J P
JP
Department of Hematology, University of Texas System Cancer Center, M.D. Anderson Hospital and Tumor Institute, Houston 77030.
Ventura
J
J
D001781:Blood Component Removal / Q000295:instrumentation*; D001792:Blood Platelets / Q000166:cytology; D001803:Blood Transfusion; D001810:Blood Volume; D002469:Cell Separation / Q000295:instrumentation*; D005434:Flow Cytometry / Q000295:instrumentation*; D006801:Humans; D010976:Platelet Count; D017713:Platelet Transfusion; D010983:Plateletpheresis / Q000295:instrumentation* / Q000379:methods