title: Determination of the aminoterminal peptide of procollagen type III and laminin P1 in serum of patients with chronic liver disease.
abstract: Serum concentration of the aminoterminal peptide of procollagen type III (P III P) and that of the high-molecular-weight glycoprotein laminin P1 (LP1) were determined by a specific radioimmunoassay (RIA) in patients with different chronic liver diseases. Besides the routine laboratory tests, histological verification of the liver samples obtained by needle biopsy and a complex hepatitis B virus marker analysis by RIA (Biomedica-Sorin), or ELISA (Behringwerke, Marburg, FRG) kits were carried out in order to set up the correct clinical diagnosis. In normal controls, the P III P and LP1 concentrations were 7.8 +/- 1.1 ng/ml (n = 10) and 0.08 +/- 0.1 units/ml (n = 7), respectively. Patients with fatty liver (n = 25) showed a significant elevation in P III P concentration (18.6 +/- 2.7 ng/ml). Such an elevation was not unequivocally demonstrated before. In this group of patients LP1 level was also increased (1.4 +/- 0.2 units/ml, n = 10). In liver cirrhosis (n = 51) both P III P and LP1 concentrations were found to be consistently elevated.
authors.affiliation: 2nd Department of Internal Medicine, Semmelweis University Medical School, Budapest.
authors.forename: M
authors.initials: M
authors.lastname: Kárteszi
authors.forename: L
authors.initials: L
authors.lastname: Szalay
authors.forename: A
authors.initials: A
authors.lastname: Cornides
authors.forename: K
authors.initials: K
authors.lastname: Dávid
authors.forename: J
authors.initials: J
authors.lastname: Fehér
mesh_terms: D015415:Biomarkers / Q000097:blood*; D005234:Fatty Liver / Q000097:blood*; D005500:Follow-Up Studies; D006514:Hepatitis B Surface Antigens / Q000032:analysis; D006801:Humans; D007797:Laminin / Q000097:blood*; D008103:Liver Cirrhosis / Q000097:blood*; D010446:Peptide Fragments / Q000097:blood*; D011347:Procollagen / Q000097:blood*; D011863:Radioimmunoassay / Q000379:methods; D011933:Reagent Kits, Diagnostic; D012016:Reference Values; D012189:Retrospective Studies
chemical_list: D015415:Biomarkers; D006514:Hepatitis B Surface Antigens; D007797:Laminin; D010446:Peptide Fragments; D011347:Procollagen; D011933:Reagent Kits, Diagnostic; C055716:laminin P1