Effects of recombinant human interleukin-1 beta on rabbit articular chondrocytes. Stimulation of prostanoid release and inhibition of cell growth.
Using an in vitro model, we characterized the production of prostaglandin E2, 6-keto-prostaglandin F1 alpha, and thromboxane B2 by normal rabbit articular chondrocytes stimulated by interleukin-1 beta. The prostanoids were produced in a dose- and time-dependent manner, which was sensitive to actinomycin D and cycloheximide. Interleukin-1 also had a pronounced, but reversible, cytostatic effect on chondrocytes, which was not attributable to prostanoid or polyamine synthesis.
Chin
J E
JE
Medical Products Department, E. I. du Pont de Nemours and Company, Glenolden, Pennsylvania.
Lin
Y A
YA
D015121:6-Ketoprostaglandin F1 alpha / Q000378:metabolism*; D000818:Animals; D002358:Cartilage, Articular / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003513:Cycloheximide / Q000494:pharmacology; D004247:DNA / Q000096:biosynthesis; D003609:Dactinomycin / Q000494:pharmacology; D015232:Dinoprostone / Q000378:metabolism*; D007375:Interleukin-1 / Q000494:pharmacology*; D008297:Male; D014176:Protein Biosynthesis; D011817:Rabbits; D011994:Recombinant Proteins; D013268:Stimulation, Chemical; D013929:Thromboxane B2 / Q000378:metabolism*
D007375:Interleukin-1; D011994:Recombinant Proteins; D003609:Dactinomycin; D013929:Thromboxane B2; D015121:6-Ketoprostaglandin F1 alpha; D004247:DNA; D003513:Cycloheximide; D015232:Dinoprostone