Isolation of multiple antibiotic resistant Enterobacteriaceae from river water.
Water samples from the Monocacy River in Frederick County, Maryland, yielded twenty-four isolates which were resistant to tetracycline (TeR, 25 micrograms/ml). Although these organisms were not initially cultured on a coliform-selective medium, twenty-two of the isolates were Gram-negative and carriers of antibiotic resistance to five or more antibiotics; erythromycin, methicillin, novobiocin, penicillin and tetracycline. Of the isolates 45% were biochemically identified as Providencia stuartii; one isolate which contained a 29.4 kilobase plasmid carried the determinant for tetracycline resistance.
Rinker
A G
AG
Department of Biology, Hood College, Frederick, Maryland 21701.
Boyd
A L
AL
Gary
N D
ND
Kundig
W
W
D000668:Ampicillin Resistance; D002702:Chloramphenicol Resistance; D003470:Culture Media; D004269:DNA, Bacterial / Q000235:genetics; D004352:Drug Resistance, Microbial; D004587:Electrophoresis, Agar Gel; D004755:Enterobacteriaceae / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D004917:Erythromycin / Q000494:pharmacology; D005618:Fresh Water; D008712:Methicillin / Q000494:pharmacology; D009675:Novobiocin / Q000494:pharmacology; D010403:Penicillin Resistance; D011815:R Factors; D013307:Streptomycin / Q000494:pharmacology; D013753:Tetracycline Resistance; D014169:Transformation, Bacterial; D014871:Water Microbiology
D003470:Culture Media; D004269:DNA, Bacterial; D009675:Novobiocin; D004917:Erythromycin; D008712:Methicillin; D013307:Streptomycin