Qualitative and quantitative analyses of phenol, phenylglucuronide, and phenylsulfate in urine and plasma by gas chromatography/mass spectrometry.
Phenol and related compounds are toxic agents to which humans are exposed. Analysis of phenolic compounds may be used as an index of exposure. In this report a method for analysis of phenol, phenylglucuronide, phenylsulfate, and related mono- and dihydroxybenzenes as their propanoate esters by gas chromatography/mass spectrometry is presented. The use of this derivatization technique increases the sensitivity of the analysis by improving chromatographic performance and introducing a traceable "reporter" group into the analyte. This method has also been adapted, by using selected ion monitoring (SIM), to the quantitative analysis of phenol.
Pierce
W M
WM
Department of Pharmacology and Toxicology, University of Louisville, School of Medicine, Kentucky 40292.
Nerland
D E
DE
D008401:Gas Chromatography-Mass Spectrometry; D005965:Glucuronates / Q000032:analysis*; D006801:Humans; D010636:Phenols / Q000032:analysis* / Q000097:blood / Q000652:urine; D013463:Sulfuric Acid Esters / Q000032:analysis*; D013464:Sulfuric Acids / Q000032:analysis*
D005965:Glucuronates; D010636:Phenols; D013463:Sulfuric Acid Esters; D013464:Sulfuric Acids; C025298:phenylglucuronide; C025208:phenylsulfate