Protein malnutrition and the febrile response in the Fischer rat.
We assessed the effect of protein deprivation on the ability of peritoneal macrophages from Fischer rats to produce interleukin-1 (IL-1) after in vitro stimulation. Pyrogenic activity of supernatants was measured by an in vivo febrile response assay. Control rats were given a 23% casein diet and protein-malnourished rats were given an 8% casein diet for 4 weeks. IL-1-containing supernatants prepared from peritoneal macrophages were injected into assay rats, whose temperatures were measured for 6 hours (delta T6). Rats injected with IL-1-containing supernatants derived from peritoneal macrophage cultures of protein-deprived rats had significantly less fever (delta T6 = 0.20 +/- 0.09 degree C) than rats injected with IL-1 containing supernatants derived from peritoneal macrophage cultures of control rats (delta T6 = 0.56 +/- 0.09 degree C), P less than .01. Protein malnutrition leads to diminished pyrogenicity of macrophage culture supernatants and may be at least partly responsible for the decreased febrile response seen in the malnourished animals.
Bradley
S F
SF
Department of Internal Medicine, VA Medical Center, Ann Arbor, MI 48105.
Kauffman
C A
CA
D000818:Animals; D004044:Dietary Proteins / Q000008:administration & dosage; D005334:Fever / Q000139:chemically induced* / Q000503:physiopathology; D007375:Interleukin-1 / Q000378:metabolism / Q000633:toxicity*; D008264:Macrophages / Q000378:metabolism*; D008297:Male; D010529:Peritoneal Cavity / Q000473:pathology; D011488:Protein Deficiency / Q000276:immunology / Q000503:physiopathology*; D051381:Rats; D011916:Rats, Inbred F344
D004044:Dietary Proteins; D007375:Interleukin-1