Regulation of the pentose phosphate cycle in bass (Dicentrarchus labrax L.) liver.
The influence in the activities of the glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase produced by the ratio changes NADPH/NADP is studied. The intracellular ratios of 20 and 10 are enough to achieve total inhibition of these two enzymes, respectively. Measurements of a number of metabolic intermediates show that the concentrations of Krebs cycle compounds are higher than those of glycolytic pathway metabolites. From a consideration of these values, the regulation of the pentose phosphate cycle mainly by the intracellular NADPH/NADP ratio, is discussed.
Medina-Puerta
M M
MM
Departamento de Bioquímica, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain.
Gallego-Iniesta
M
M
Garrido-Pertierra
A
A
D000818:Animals; D001492:Bass; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism*; D008099:Liver / Q000201:enzymology*; D009249:NADP / Q000378:metabolism*; D010427:Pentose Phosphate Pathway; D010734:Phosphogluconate Dehydrogenase / Q000378:metabolism*
D009249:NADP; D010734:Phosphogluconate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase