title: Histochemical localization of IGF-I and IGF-II mRNA in the rat between birth and adulthood.
abstract: We describe the postnatal ontogeny and localization of insulin-like growth factors I and II (IGF-I and -II) in the rat. We have used oligodeoxyribonucleotide probes for in situ hybridization (hybridization histochemistry) and for Northern blotting. IGF-II mRNA is strongly expressed in liver, skeletal muscle, perichondrium, leptomeninges and choroid plexus of the newborn. Demonstrable levels fall dramatically in the liver at 18-20 days postnatally but persist for longer periods in muscle and remain undiminished throughout life in the pia/choroid plexus, indicating that different control mechanisms operate in these tissues. IGF-I mRNA is predominantly found in the liver. Its level in this organ rises well before levels of IGF-II fall. This suggests that distinct factors govern the expression of IGF-I and -II genes.
authors.affiliation: Department of Anatomy, University of Leicester, UK.
authors.forename: F
authors.initials: F
authors.lastname: Beck
authors.forename: N J
authors.initials: NJ
authors.lastname: Samani
authors.forename: S
authors.initials: S
authors.lastname: Byrne
authors.forename: K
authors.initials: K
authors.lastname: Morgan
authors.forename: R
authors.initials: R
authors.lastname: Gebhard
authors.forename: W J
authors.initials: WJ
authors.lastname: Brammar
mesh_terms: D000818:Animals; D001345:Autoradiography; D015152:Blotting, Northern; D001923:Brain Chemistry; D007334:Insulin-Like Growth Factor I / Q000235:genetics*; D007335:Insulin-Like Growth Factor II / Q000235:genetics*; D008099:Liver / Q000032:analysis* / Q000254:growth & development; D024510:Muscle Development; D009132:Muscles / Q000032:analysis*; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D013002:Somatomedins / Q000235:genetics*
chemical_list: D012333:RNA, Messenger; D013002:Somatomedins; D007334:Insulin-Like Growth Factor I; D007335:Insulin-Like Growth Factor II