title: Organization and nucleotide sequence of the broad bean chloroplast genes trnL-UAG, ndhF and two unidentified open reading frames.
abstract: We have determined the nucleotide sequence of a 6.9 kbp BamHI-XbaI fragment of broad bean chloroplasts. Part of this fragment (subfragment BglII-ClaI) is known to contain three tRNA genes (trnL-CAA, trnL-UAA and trnF). We have now further identified a gene coding for the third tRNA(Leu) isoacceptor (trnL-UAG) which is located close to trnF. The BamHI-XbaI fragment also contains the gene for subunit 5 of NADH dehydrogenase (ndhF) and two unidentified open reading frames (ORFx and ORF48). ORFx shares a high sequence homology with the long reading frames of tobacco (ORF1708), spinach (ORF2131), and liverwort (ORF2136), while ORF48 shares sequence homology with ORF69 of liverwort and ORF55 of tobacco.
authors.affiliation: Institut de Biologie Moléculaire des Plantes, Strasbourg, France.
authors.forename: F
authors.initials: F
authors.lastname: Herdenberger
authors.forename: J H
authors.initials: JH
authors.lastname: Weil
authors.forename: A
authors.initials: A
authors.lastname: Steinmetz
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002736:Chloroplasts; D004247:DNA / Q000235:genetics*; D007887:Fabaceae / Q000235:genetics*; D008969:Molecular Sequence Data; D010946:Plants, Medicinal; D011401:Promoter Regions, Genetic; D012345:RNA, Transfer, Amino Acid-Specific / Q000235:genetics*; D012356:RNA, Transfer, Leu / Q000235:genetics*; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
chemical_list: D012345:RNA, Transfer, Amino Acid-Specific; D012356:RNA, Transfer, Leu; D004247:DNA