[Anticancer drug screening test for human osteosarcoma transplanted into nude mice].
Osteosarcoma tissue was transplanted into the subcutis of nude mice, and an anticancer drug was injected into the abdomen of the mice. The effects of Cis-platinum, SF-1739HP, Melphalan, Peleomycin and Aclacinomycin were tested. Only Cis-platinum had previously been used in the treatment of osteosarcoma. As the conventional method of evaluation, tumor weight change was recorded along the time course. As a new evaluation method, toluidine-blue was added into the tumor cell suspension prepared from the tumor tissue in the back of nude mice. By the intensity of staining, the tumor cells in suspension were classified into 3 categories; strongly-positive, weakly-positive and negative. Results of evaluation by the staining method were similar to those by measurement of tumor weight. Cis-platinum proved to be most effective, followed in decreasing order by SF-1739HP, Melphalan, Pepleomycin and Aclacinomycin. In conclusion, the staining method is simple and useful for screening the anti-cancer effects of drugs.
Mochizuki
K
K
Department of Orthopaedic Surgery, Kyorin University School of Medicine, Tokyo, Japan.
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor / Q000379:methods*; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D012516:Osteosarcoma / Q000473:pathology*; D014048:Tolonium Chloride; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000970:Antineoplastic Agents; D014048:Tolonium Chloride