title: B cell stimulatory factor 1 induces lobster agglutinin 1-separated mouse thymocytes to express cytotoxic T lymphocyte activity.
abstract: Mouse thymocytes low in surface sialic acid were prepared by using the lectin lobster agglutinin 1 (LAg1). These LAg1-thymocytes do not become CTL when incubated with Con A or Con A plus mouse rIL-2, whereas unseparated thymocytes and thymocytes with high levels of surface sialic acid develop good levels of polyclonal CTL activity under these conditions. However, LAg1- thymocytes developed high levels of CTL activity when incubated with B cell stimulatory factor-1 (BSF-1), provided as the supernatant of the rBSF-1-secreting T cell hybridoma D9-C1.12.17. Affinity-purified BSF-1 from D9-C1 supernatant and rBSF-1 also stimulated these cells to become CTL, but they were not as active as the D9-C1 supernatant. The ability of D9-C1 supernatant and of affinity-purified BSF-1 to induce CTL activity was inhibited by the anti-BSF-1 mAb 11B11. Moreover, this mAb inhibited the ability of 24-h Con A-stimulated spleen cell supernatant to induce these cells to express CTL activity. 11B11 also inhibited LAg1+ thymocytes from becoming CTL when stimulated with Con A alone. These experiments suggest that BSF-1 is required for LAg1- and LAg1+ thymocytes to become CTL.
authors.affiliation: Department of Medicine, National Jewish Center for Immunology and Respiratory Medicine, Denver, CO 80206.
authors.forename: J M
authors.initials: JM
authors.lastname: Collins
authors.forename: L B
authors.initials: LB
authors.lastname: Justement
authors.forename: K E
authors.initials: KE
authors.lastname: Stedman
authors.forename: A
authors.initials: A
authors.lastname: Zlotnik
authors.forename: P A
authors.initials: PA
authors.lastname: Campbell
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000502:physiology; D002469:Cell Separation; D002474:Cell-Free System; D003208:Concanavalin A / Q000037:antagonists & inhibitors; D003602:Cytotoxicity, Immunologic; D005260:Female; D006825:Hybridomas / Q000502:physiology; D007166:Immunosuppressive Agents / Q000502:physiology; D007376:Interleukin-2 / Q000494:pharmacology; D015847:Interleukin-4; D007378:Interleukins / Q000276:immunology / Q000502:physiology*; D037102:Lectins; D008297:Male; D051379:Mice; D019158:N-Acetylneuraminic Acid; D008121:Nephropidae / Q000276:immunology*; D010641:Phenotype; D051381:Rats; D011919:Rats, Inbred Strains; D011994:Recombinant Proteins / Q000502:physiology; D063265:Sialic Acid Binding Immunoglobulin-like Lectins; D012794:Sialic Acids; D013602:T-Lymphocytes, Cytotoxic / Q000145:classification / Q000276:immunology* / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D007166:Immunosuppressive Agents; D007376:Interleukin-2; D007378:Interleukins; D037102:Lectins; D011994:Recombinant Proteins; D063265:Sialic Acid Binding Immunoglobulin-like Lectins; D012794:Sialic Acids; D003208:Concanavalin A; D015847:Interleukin-4; D019158:N-Acetylneuraminic Acid