Nitric oxide release from the isolated guinea pig heart.
Nitric oxide (NO) release from isolated guinea pig hearts into the coronary effluent perfusate was measured with a specific spectrophotometric assay. Nitric oxide was continuously released under basal conditions at a rate of 216 +/- 36 pmol/min. Bradykinin (5 X 10(-10)-10(-8) M) stimulated the release of nitric oxide in a dose-dependent manner. The start of NO release preceded coronary vasodilation by 5 +/- 1 s. Our results suggest that the endogenous formation of nitric oxide is quantitatively sufficient to influence the coronary vascular tone and thus may play a significant role in the regulation of coronary vascular resistance.
Kelm
M
M
Institute of Physiology, University of Düsseldorf, F.R.G.
Schrader
J
J
D000818:Animals; D001920:Bradykinin / Q000494:pharmacology; D003331:Coronary Vessels / Q000187:drug effects; D006168:Guinea Pigs; D006321:Heart / Q000187:drug effects; D066298:In Vitro Techniques; D009206:Myocardium / Q000378:metabolism*; D009569:Nitric Oxide / Q000378:metabolism*; D010108:Oxyhemoglobins / Q000378:metabolism; D014655:Vascular Resistance / Q000187:drug effects
D010108:Oxyhemoglobins; D009569:Nitric Oxide; D001920:Bradykinin