title: Functional states of neutrophils as suggested by whole blood chemiluminescence.
abstract: Luminol-enhanced chemiluminescence (CL) of whole blood was examined in order to distinguish between activation states of phagocytic cells. The CL response of these cells was provoked by a phagocytic stimulus--polystyrene particles. Four functional states of phagocytes were proposed: "resting", "stand by", "activated" and "exhausted". The distinction was done on the basis of extent of the CL response to the particles, time pattern of the process, inhibition of CL by plasma and appearance of spontaneous light emission. Freshly drawn blood of healthy individuals exhibits the "resting" profile of CL, but that of patients with bacterial infection reveals CL patterns ascribed in this paper to the "stand by", "activated" or "exhausted" states of phagocytes. The "stand by", "activated" and "exhausted" behaviour of phagocytes in extravasated blood may be induced by preincubation of blood, stimulation with saline extract of Escherichia coli or N-formyl-Met-Leu-Phe, and by some manipulations involved in preparation of the purified neutrophils.
authors.affiliation: Institute of Medical Biochemistry, N. Copernicus Academy of Medicine, Kraków, Poland.
authors.forename: J M
authors.initials: JM
authors.lastname: Zgliczyński
authors.forename: E
authors.initials: E
authors.lastname: Kwasnowska
authors.forename: T
authors.initials: T
authors.lastname: Stelmaszyńska
authors.forename: E
authors.initials: E
authors.lastname: Olszowska
authors.forename: S
authors.initials: S
authors.lastname: Olszowski
authors.forename: J M
authors.initials: JM
authors.lastname: Knapik
mesh_terms: D001424:Bacterial Infections / Q000097:blood; D001770:Blood Bactericidal Activity; D006801:Humans; D008163:Luminescent Measurements; D009504:Neutrophils / Q000502:physiology*; D010587:Phagocytosis