title: Selective growth response to IL-3 of a human leukaemic cell line with megakaryoblastic features.
abstract: A new human leukaemic cell line (M-O7) with the phenotypic characteristics of CFU-mega is described. Its cells are positive for T200 leucocyte common antigen (LCA) and negative with MAbs recognizing T and B cells and mature myelomonocytic antigens. In contrast, they react with MAbs recognizing antigenic determinants common to multi-lineage (CD13, CD33, CD34) and to bipotent erythromegakaryoblastic (CD36, H25) haemopoietic precursors, and with MAbs specific for platelet glycoproteins (CD41w, CD42w). A small proportion (10%) of the cells were large and multinucleated, and on electron-microscopy examination showed peripheral splitting of platelet-like cytoplasm particles. When transferred to a serum-free Iscove modified Dulbecco's medium supplemented with human insulin and transferrin, M-O7 cells stop proliferating. Of the haemopoietic growth factors tested for their ability to restore the proliferative activity of this quiescent population, only rH IL-3 proved effective. Moreover, it also increased the cloning efficiency in methylcellulose more than any other CSFs. The M-O7 cell line may provide a valuable tool for the biological assay of IL-3, and a model for biochemical studies of the megakaryocytic lineage.
authors.affiliation: Istituto di Medicina Interna, Università de Torino, Italy.
authors.forename: G C
authors.initials: GC
authors.lastname: Avanzi
authors.forename: P
authors.initials: P
authors.lastname: Lista
authors.forename: B
authors.initials: B
authors.lastname: Giovinazzo
authors.forename: R
authors.initials: R
authors.lastname: Miniero
authors.forename: G
authors.initials: G
authors.lastname: Saglio
authors.forename: G
authors.initials: G
authors.lastname: Benetton
authors.forename: R
authors.initials: R
authors.lastname: Coda
authors.forename: G
authors.initials: G
authors.lastname: Cattoretti
authors.forename: L
authors.initials: L
authors.lastname: Pegoraro
mesh_terms: D002460:Cell Line; D003114:Colony-Forming Units Assay; D006801:Humans; D007377:Interleukin-3 / Q000494:pharmacology*; D007947:Leukemia, Megakaryoblastic, Acute / Q000276:immunology / Q000473:pathology*; D008854:Microscopy, Electron
chemical_list: D007377:Interleukin-3