Two new esterase D (ESD) variants revealed by isoelectric focusing in agarose gel.
Using isoelectric focusing in thin-layer agarose gel (AGIF) with the narrow pH range of 4.5-5.4, a high resolution of esterase D (ESD) isozyme banding patterns has been achieved. Some variant phenotypes which could not be distinguished from common ESD types by conventional electrophoresis have shown different patterns after AGIF. The IEF method permitted the distinction of two further variants in the ESD system, tentatively named ESD Rehren and ESD Ravensburg. We recommend, therefore, that for the classification of ESD phenotypes a high resolution IEF technique should be used.
Weidinger
S
S
Institut für Anthropologie und Humangenetik der Universität München, Federal Republic of Germany.
Henke
J
J
D000483:Alleles; D043182:Carboxylesterase; D002265:Carboxylic Ester Hydrolases / Q000032:analysis / Q000235:genetics*; D004592:Electrophoresis, Starch Gel; D006801:Humans; D007525:Isoelectric Focusing; D010641:Phenotype
D002265:Carboxylic Ester Hydrolases; D043182:Carboxylesterase; C020118:ESD protein, human