title: Induction of a systemic lupus erythematosus-like disease in mice by a common human anti-DNA idiotype.
abstract: Systemic lupus erythematosus (SLE) is considered to be the quintessential autoimmune disease. It has not been possible to induce SLE in animal models by DNA immunization or by challenge with anti-DNA antibodies. We herewith report a murine model of SLE-like disease induced by immunization of C3H.SW female mice with a common human monoclonal anti-DNA idiotype (16/6 idiotype). Following a booster injection with the 16/6 idiotype, high levels of murine anti-16/6 and anti-anti-16/6 antibodies (associated with anti-DNA activity) were detected in the sera of the immunized mice. Elevated titers of autoantibodies reacting with DNA, poly(I), poly(dT), ribonucleoprotein, autoantigens [Sm, SS-A (Ro), and SS-B (La)], and cardiolipin were noted. The serological findings were associated with increased erythrocyte sedimentation rate, leukopenia, proteinuria, immune complex deposition in the glomerular mesangium, and sclerosis of the glomeruli. The immune complexes in the kidneys were shown to contain the 16/6 idiotype. This experimental SLE-like model may be used to elucidate the mechanisms underlying SLE.
authors.affiliation: Department of Chemical Immunology, Weizmann Institute of Science, Rehovot, Israel.
authors.forename: S
authors.initials: S
authors.lastname: Mendlovic
authors.forename: S
authors.initials: S
authors.lastname: Brocke
authors.forename: Y
authors.initials: Y
authors.lastname: Shoenfeld
authors.forename: M
authors.initials: M
authors.lastname: Ben-Bassat
authors.forename: A
authors.initials: A
authors.lastname: Meshorer
authors.forename: R
authors.initials: R
authors.lastname: Bakimer
authors.forename: E
authors.initials: E
authors.lastname: Mozes
mesh_terms: D000818:Animals; D000974:Antibodies, Antinuclear / Q000276:immunology*; D001327:Autoimmune Diseases / Q000209:etiology* / Q000276:immunology; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D008180:Lupus Erythematosus, Systemic / Q000209:etiology*; D051379:Mice; D008809:Mice, Inbred C3H / Q000276:immunology
chemical_list: D000974:Antibodies, Antinuclear; D007130:Immunoglobulin Idiotypes