Interleukin-1 induces c-fos protooncogene expression in cultured human endothelial cells.
In the present study we have evaluated the expression of c-fos protooncogene in normal human endothelial cells (HEC) by Northern blot analysis. HEC do not show neither constitutive nor cycloheximide-induced expression of c-fos protooncogene. When HEC were treated with cytokines known to modulate a number of specialized functions of these cells, we observed that, unlike interferon-gamma, interleukin-1 (IL-1) and tumor necrosis factor (TNF) were able to induce appreciable levels of c-fos in HEC. Both IL-1 alpha and IL-1 beta induced c-fos transcripts in HEC. Maximal levels of c-fos mRNA induced by IL-1 were found after 1 hour of treatment, with undetectable levels at 4 and 7 hours. c-fos induction in HEC by IL-1 and TNF may play a role in the acquisition of functional properties induced in HEC by these cytokines.
Colotta
F
F
Istituto di Ricerche Farmacologiche Mario Negri, Milan, Italy.
Lampugnani
M G
MG
Polentarutti
N
N
Dejana
E
E
Mantovani
A
A
D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D004730:Endothelium, Vascular / Q000187:drug effects*; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology*; D011519:Proto-Oncogenes; D012333:RNA, Messenger / Q000032:analysis
D007375:Interleukin-1; D012333:RNA, Messenger