Cloning and analysis of the HaeIII and HaeII methyltransferase genes.
The HaeIII methyltransferase (MTase) gene from Haemophilus aegyptius (recognition sequence: 5'-GGCC-3') was cloned into Escherichia coli in the plasmid vector pBR322. The gene was isolated on a single EcoRI fragment and on a single HindIII fragment. Clones carrying additional adjacent fragments were found to code also for the HaeII restriction endonuclease and HaeII modification MTase (recognition sequence: 5'-PuGCGCPy-3'). The sequence of the HaeIII modification gene was determined. The inferred amino acid sequence of the protein was found to share extensive similarity with other sequenced m5C-MTases. The central 'non-conserved' region of the M.HaeIII MTase, thought to form the nucleotide sequence-specificity domain, is almost identical to that of the M.BsuRI, M.BspRI and M.NgoPII MTases, which also recognize the sequence 5'-GGCC-3'.
Slatko
B E
BE
New England Biolabs, Inc., Beverly, MA 01915.
Croft
R
R
Moran
L S
LS
Wilson
G G
GG
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D003001:Cloning, Molecular; D015257:DNA-Cytosine Methylases / Q000235:genetics* / Q000378:metabolism; D005796:Genes; D005798:Genes, Bacterial; D006190:Haemophilus / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012689:Sequence Homology, Nucleic Acid
D001426:Bacterial Proteins; D011994:Recombinant Proteins; C059638:DNA modification methylase HaeII; D015257:DNA-Cytosine Methylases; C027823:Cytosine 5-methyltransferase