Plasma sorbitol dehydrogenase determination in experimental hepatotoxicity using the Abbott Bichromatic Analyzer.
An automated single reagent micro-assay for the determination of sorbitol dehydrogenase (EC 1.1.1.14) catalytic activity concentration in mouse plasma was developed for the Abbott Bichromatic Analyzer (ABA-100). The kinetic rate determination was linear up to 250 U/l, had a CV of 2.65%, and required only 25 microliters of sample. Experimentally induced hepatotoxicity in the mouse by acetaminophen produced a dose dependent increase in blood sorbitol dehydrogenase activity.
Pakuts
A P
AP
Bureau of Drug Research, Drug Toxicology Division, Tunney's Pasture, Ottawa, Ontario, Canada.
Whitehouse
L W
LW
Paul
C J
CJ
D000082:Acetaminophen / Q000633:toxicity*; D000818:Animals; D007700:Kinetics; D007064:L-Iditol 2-Dehydrogenase / Q000097:blood*; D008099:Liver / Q000187:drug effects / Q000473:pathology*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D013401:Sugar Alcohol Dehydrogenases / Q000097:blood*
D000082:Acetaminophen; D013401:Sugar Alcohol Dehydrogenases; D007064:L-Iditol 2-Dehydrogenase