Effective immunization against cutaneous leishmaniasis with defined membrane antigens reconstituted into liposomes.
The abundant Leishmania promastigote surface Ag gp63 and Leishmania promastigote lipophosphoglycan were reconstituted into liposomes and used as a vaccine against the agent of New World cutaneous leishmaniasis, Leishmania mexicana. The Ag were inoculated s.c., i.p., and i.v. into CBA/ca and BALB/c mice. Even at low Ag dosages, 8 to 10 micrograms/mouse, the Ag induced appreciable levels of protection. In CBA/ca mice complete protection was obtained by s.c. inoculation of antigen-containing liposomes. Protection could be transferred with T cells to naive mice. Interestingly, the Ag-containing liposomes did not cause the disease exacerbation observed in previous vaccine studies with crude parasite extracts.
Russell
D G
DG
Max-Planck-Institut für Biologie, Tübingen, Federal Republic of Germany.
Alexander
J
J
D000818:Animals; D000953:Antigens, Protozoan / Q000008:administration & dosage* / Q000276:immunology; D005260:Female; D007114:Immunization; D007116:Immunization, Passive; D007894:Leishmania mexicana / Q000276:immunology*; D007896:Leishmaniasis / Q000276:immunology / Q000517:prevention & control*; D008081:Liposomes / Q000008:administration & dosage; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D008808:Mice, Inbred CBA / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D014612:Vaccines / Q000008:administration & dosage* / Q000276:immunology
D000953:Antigens, Protozoan; D008081:Liposomes; D014612:Vaccines