Interleukin 1 enhances growth factor-dependent proliferation of the clonogenic cells in acute myeloblastic leukemia and of normal human primitive hemopoietic precursors.
IL-1 is released by activated monocytes and is thought to be a key mediator of the host immune response. The availability of the purified and, more recently, recombinant IL-1 has allowed the characterization of other biological properties of this molecule. Thus, IL-1 is thought to have the same properties as hemopoietic 1, a growth factor that has been shown to act on primitive murine hemopoietic cells. Here we report that rIL-1 acts synergistically with granulocyte/macrophage CSF (GM-CSF) or granulocyte CSF in the stimulation of clonogenic cells from many patients with acute myeloblastic leukemia (AML). Although IL-1 by itself has no effect on AML blasts, it can support colony formation under conditions where there is detectable production of endogenous GM-CSF. IL-1 also promotes the growth of multipotential progenitors from normal human bone marrow cells in the presence of GM-CSF. These observations support the hypothesis that in the hemopoietic system, IL-1 has a selective effect on primitive precursors.
Hoang
T
T
Clinical Research Institute of Montreal, Quebec, Canada.
Haman
A
A
Goncalves
O
O
Letendre
F
F
Mathieu
M
M
Wong
G G
GG
Clark
S C
SC
D001854:Bone Marrow Cells; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D003115:Colony-Stimulating Factors / Q000276:immunology / Q000494:pharmacology*; D004357:Drug Synergism; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000187:drug effects; D006801:Humans; D007106:Immune Sera; D007375:Interleukin-1 / Q000235:genetics / Q000502:physiology*; D007700:Kinetics; D015470:Leukemia, Myeloid, Acute / Q000473:pathology*; D009693:Nucleic Acid Hybridization; D011994:Recombinant Proteins / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000166:cytology* / Q000187:drug effects; D014410:Tumor Stem Cell Assay
D003115:Colony-Stimulating Factors; D007106:Immune Sera; D007375:Interleukin-1; D011994:Recombinant Proteins