Purification and crystallization of hemorrhagic factor, HR2b, from the venom of Trimeresurus flavoviridis (habu).
The hemorrhagic factor, HR2b, was purified from the venom of Trimeresurus flavoviridis (habu) by a combination of gel filtration, cation exchange column chromatography and high performance liquid chromatography. The purified HR2b was homogeneous by the criteria of ultracentrifugation and SDS-disc electrophoresis. The mol. wt of HR2b was 18,000 and 18,500 by gel filtration on Sephadex G-50 and by SDS-disc electrophoresis, respectively, indicating a monomer structure for the hemorrhagic factor. Crystals of HR2b, taking the form of thin plates, were obtained in the presence of ammonium sulfate.
Yonaha
K
K
Department of Agricultural Chemistry, University of The Ryukyus, Okinawa, Japan.
Iha
M
M
Tomihara
Y
Y
Nozaki
M
M
Yamakawa
M
M
Kamura
T
T
Toyama
S
S
D000818:Animals; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003435:Crotalid Venoms / Q000032:analysis / Q000302:isolation & purification / Q000633:toxicity*; D004589:Electrophoresis, Disc
D003435:Crotalid Venoms; C052578:hemorrhagic toxin HR-2b