[Ultrastructural changes in the plasma membrane of A-431 cells exposed to epidermal growth factor].
The plasma membrane ultrastructural changes after the action of epidermal growth factor were studied in A-431 cells using freeze-fracture methods. The incubation with EGF (100 ng/ml, 0 degree C, 60 min) led to a decrease in density of intramembrane particles on the P surface of ventral cell membrane, while the number of coated pits increased there. The revealed effects of EGF may be related to direct consequences of EGF-receptor complex formation, because all the temperature dependent steps of its processing were blocked. The data obtained testify to an active involvement of the membrane ventral surface in the formation of cell response towards growth factors.
Romanov
V I
VI
Sorokin
A B
AB
Nesterov
A M
AM
Komissarchik
Ia Iu
IaIu
D002294:Carcinoma, Squamous Cell / Q000188:drug therapy / Q000648:ultrastructure*; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects / Q000648:ultrastructure; D004305:Dose-Response Relationship, Drug; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D005614:Freeze Fracturing; D006801:Humans; D008854:Microscopy, Electron; D013499:Surface Properties; D014407:Tumor Cells, Cultured
D004815:Epidermal Growth Factor
Izmenenie ul'trastruktury plazmaticheskoĭ membrany kletok A-431 pri deĭstvii épidermal'nogo faktora rosta.