title: A simple enzyme-linked immunosorbent assay for sex hormone-binding globulin.
abstract: A simple enzyme-linked immunosorbent assay (ELISA) for sex hormone-binding globulin (SHBG) has been developed. Polyclonal antibody raised to SHBG purified to homogeneity was employed. The ELISA, which may be performed in under 4 h, shows no cross-reactivity with other serum proteins, has a sensitivity of less than 1.2 fmol per sample, demonstrates excellent correlation with ligand-binding techniques (r = 0.996; p less than 0.0001), and has intra- and inter-assay coefficients of variation of between 5-9% and 7-11% respectively.
authors.affiliation: Tumour Biology Unit, King's College School of Medicine and Dentistry, London, UK.
authors.forename: M J
authors.initials: MJ
authors.lastname: Iqbal
authors.forename: A
authors.initials: A
authors.lastname: Forbes
authors.forename: S H
authors.initials: SH
authors.lastname: Valyani
authors.forename: T P
authors.initials: TP
authors.lastname: Corbishley
authors.forename: M L
authors.initials: ML
authors.lastname: Wilkinson
mesh_terms: D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D008024:Ligands; D011817:Rabbits; D015203:Reproducibility of Results; D012738:Sex Hormone-Binding Globulin / Q000032:analysis*
chemical_list: D008024:Ligands; D012738:Sex Hormone-Binding Globulin