Chromosomal localization of satellite DNA sequences among 22 species of felids and canids (Carnivora).
In situ hybridization was carried out using cloned satellite DNAs from the domestic cat and domestic dog as probes to metaphase chromosomes from 12 species of felids and 10 species of canids. Autoradiographic silver grains along metaphase chromosomes were counted and analyzed with regard to the mean number of grains per cell in each species, their chromosomal location, and their presence or absence on specific autosomes or sex chromosomes, where known. Among the felids and canids there was a 7.6- and 8.9-fold statistically significant difference, respectively, in the mean number of grains per cell between the species having the minimum and maximum values. Among the felids, most grains occurred on the telomeres of D- and E-group chromosomes, although departures from this general pattern also occurred. For example, the Asian golden cat and the Bornean bay cat showed substantial labeling at the centromeric region of chromosome A1, and a number of species showed some labeling at the short-arm telomeres of B-group chromosomes. Among the canids, about 90% of all grains were located at autosomal centromeres, and grains were absent from the sex chromosomes. Grains are usually distributed at chromosomal locations that stain C-band positive; however, certain C-band-positive regions without grains probably do not contain the particular satellites studied here.
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Biological Carcinogenesis Development Program, Program Resources, Inc., Frederick Cancer Research Facility, MD.
Fanning
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TG
Wayne
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RK
O'Brien
S J
SJ
D000818:Animals; D001345:Autoradiography; D001483:Base Sequence; D002335:Carnivora / Q000235:genetics*; D002874:Chromosome Mapping; D002875:Chromosomes / Q000032:analysis / Q000648:ultrastructure; D003001:Cloning, Molecular; D004276:DNA, Satellite / Q000032:analysis*; D006822:Hybrid Cells / Q000032:analysis / Q000648:ultrastructure; D008677:Metaphase; D009693:Nucleic Acid Hybridization; D012834:Silver; D013223:Statistics as Topic
D004276:DNA, Satellite; D012834:Silver