title: Determination of molecular weight of native proteins by polyacrylamide gradient gel electrophoresis.
abstract: An improved method for the estimation of molecular weights of native proteins by polyacrylamide gel electrophoresis, in 9 cm x 9 cm x 0.05 mm 4-20% T fabric reinforced gradient gels, is described. Plotting the logarithm of the relative mobilities of proteins versus gel concentrations produces lines whose slopes are related to molecular weights.
authors.affiliation: Kyoritsu College of Pharmacy, Tokyo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Nishizawa
authors.forename: N
authors.initials: N
authors.lastname: Kita
authors.forename: S
authors.initials: S
authors.lastname: Okimura
authors.forename: E
authors.initials: E
authors.lastname: Takao
authors.forename: Y
authors.initials: Y
authors.lastname: Abe
mesh_terms: D002138:Calibration; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D006801:Humans; D008970:Molecular Weight; D011506:Proteins / Q000032:analysis*; D014325:Tromethamine
chemical_list: D011506:Proteins; D014325:Tromethamine