Impaired proliferation response after PDGF induction in fibroblasts from Hutchinson-Guilford Progeria syndrome.
Fibroblasts from a Hutchinson-Guilford Progeria Syndrome (HGPS) patient were compared to normal human fibroblasts to determine if differences existed in growth factor mediated cell proliferation. Cultures of progeric fibroblasts were exposed individually to platelet-derived growth factor (PDGF), epidermal growth factor (EGF), platelet poor plasma (PPP) and fetal bovine serum (FBS). Autoradiographic studies using 3H thymidine showed that progeric fibroblasts had similar labeling indices relative to controls after exposure to FBS and EGF. In contrast, progeric cells made competent with PDGF and later treated with 5% PPP had a significantly lower labeling index. This and preliminary observations on fos RNA accumulation suggests the possible existence of a genetic defect in HGPS fibroblasts.
Stachniak
J B
JB
Department of Cellular and Structural Biology, University of Texas Health Science Center, San Antonio 78284.
Sharp
Z D
ZD
Pool
T B
TB
Cameron
I L
IL
D001345:Autoradiography; D001792:Blood Platelets / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects*; D002460:Cell Line; D002648:Child; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005260:Female; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects; D006801:Humans; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology*; D011371:Progeria / Q000473:pathology*; D013936:Thymidine
D010982:Platelet-Derived Growth Factor; D004815:Epidermal Growth Factor; D013936:Thymidine