title: Multigene families of Cellulomonas flavigena encoding endo-beta-1,4-glucanases (CM-cellulases).
abstract: Multiple genes coding for endo-beta-1,4-glucanases (CM-cellulases) have been isolated from a newly discovered highly cellulolytic strain of Cellulomonas flavigena. Clones of C. flavigena DNA were isolated in Escherichia coli and screened for gene expression on CM-cellulose plates staining with congo red. Six clones produced CM-cellulase activity as detected in liquid assays, and on activity gels. They fell into three groups within which the sequences cross-hybridised. There were small differences in the pH and temperature optima of the enzymes encoded by representatives of the three groups of clones.
authors.affiliation: Department of Genetics, Trinity College, Dublin, Ireland.
authors.forename: M W
authors.initials: MW
authors.lastname: Akhtar
authors.forename: M
authors.initials: M
authors.lastname: Duffy
authors.forename: B C
authors.initials: BC
authors.lastname: Dowds
authors.forename: M C
authors.initials: MC
authors.lastname: Sheehan
authors.forename: D J
authors.initials: DJ
authors.lastname: McConnell
mesh_terms: D001419:Bacteria / Q000235:genetics*; D002480:Cellulase / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D005786:Gene Expression Regulation; D006863:Hydrogen-Ion Concentration; D005810:Multigene Family; D010957:Plasmids; D012689:Sequence Homology, Nucleic Acid; D013696:Temperature
chemical_list: D004247:DNA; D002480:Cellulase