T cell localization in rabbit models of acute and chronic experimental hypersensitivity pneumonitis.
Cell-mediated hypersensitivity has been increasingly implicated in immunologic diseases of the lung, including hypersensitivity pneumonitis (HSP) (extrinsic allergic alveolitis). We used a T cell-specific monoclonal antibody (L11/135) to localize T cells in the parenchyma and bronchus-associated lymphoid tissue of ethanol-fixed, paraffin-embedded lung sections in rabbit models of experimental HSP to define further their possible role in pathogenesis. T cells appeared within 4 hours in early lesions of rabbit models of acute HSP and heavily infiltrated alveolitis lesions at 8 and 24 hours after aerosol challenge. T cells were also present in lesions of rabbit models with chronic alveolitis and occurred peripherally in granulomas. Variable aggregate and follicular forms of bronchus-associated lymphoid tissue rich in T cells occurred in both experimental and control animals. Our findings document early and continuing presence of T cells in lesions in rabbit models of experimental HSP.
Upadrashta
B
B
Department of Internal Medicine, University of Iowa, Iowa City.
Croom
J
J
Kopp
W C
WC
Withers
J
J
Richerson
H B
HB
D000208:Acute Disease; D000336:Aerosols; D000542:Alveolitis, Extrinsic Allergic / Q000276:immunology / Q000473:pathology*; D000818:Animals; D000941:Antigens / Q000008:administration & dosage; D001065:Appendix / Q000473:pathology; D002465:Cell Movement; D002908:Chronic Disease; D004195:Disease Models, Animal; D005260:Female; D010581:Peyer's Patches / Q000473:pathology; D011650:Pulmonary Alveoli / Q000473:pathology; D011817:Rabbits; D013601:T-Lymphocytes / Q000473:pathology*
D000336:Aerosols; D000941:Antigens