Uptake of antibody directed cytotoxic liposomes by CD3 on human T cells.
Using polyclonal human T cells and anti-CD3 monoclonal antibodies we have shown that small unilamellar liposomes covalently coupled with protein A become bound to T cells and not to B cells and that the binding was a specific liposome-antibody-receptor interaction. Intracellular delivery of liposome contents was demonstrated by the use of encapsulated carboxyfluorescein and flow cytometry and the transfer of membrane-bound liposomal carboxyfluorescein was virtually complete in 30 min. Liposomes containing methotrexate inhibited the growth of PHA-stimulated peripheral blood lymphocytes by 90%, after 48 h incubation. Potential applications are proposed in the study of the behaviour of surface membrane components and in T cell depletion and purging of bone marrow.
Gray
A G
AG
Department of Clinical Haematology, University College London, UK.
Morgan
J
J
Linch
D C
DC
Huehns
E R
ER
D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology; D002478:Cells, Cultured; D006801:Humans; D000922:Immunotoxins / Q000378:metabolism*; D008081:Liposomes / Q000378:metabolism*; D008727:Methotrexate / Q000494:pharmacology; D008856:Microscopy, Fluorescence; D013205:Staphylococcal Protein A / Q000378:metabolism; D013601:T-Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D013696:Temperature
D000945:Antigens, Differentiation, T-Lymphocyte; D000922:Immunotoxins; D008081:Liposomes; D013205:Staphylococcal Protein A; D008727:Methotrexate