Tissue specific methylation of human Y chromosomal DNA sequences.
This report describes two moderately repetitive human Y chromosomal DNA sequences isolated from a flow sorted Y chromosonal library. These sequences are present in XY male and XY female DNAs but absent in XX male and XX female DNAs. Genomic Southern blot analysis against DNAs isolated from different tissues showed tissue specific DNA methylation patterns. In contrast to the 2.1 kb Hae III repeats which are hypomethylated in sperm DNA, the moderately repetitive sequences used in this study are highly methylated in sperm, less methylated in blood and brain and least methylated in placental DNA.
Nishioka
Y
Y
Department of Biology, McGill University, Montreal, Quebec, Canada.
D001483:Base Sequence; D001923:Brain Chemistry; D004247:DNA / Q000378:metabolism*; D015342:DNA Probes; D005260:Female; D006801:Humans; D008297:Male; D008745:Methylation; D009693:Nucleic Acid Hybridization; D009928:Organ Specificity; D010920:Placenta / Q000032:analysis; D011247:Pregnancy; D012091:Repetitive Sequences, Nucleic Acid; D013094:Spermatozoa / Q000032:analysis; D014998:Y Chromosome
D015342:DNA Probes; D004247:DNA