Increase of sialyltransferase activity in the small intestine following thermal injury in rats.
The acute phase protein response following inflammation is associated with an increased total protein-bound carbohydrate content in plasma in the form of glycoproteins. Glycosyltransferases in liver may serve as a regulator of this increased glycosylation activity in the plasma and may also serve as a marker for the acute phase response. Sialyltransferase is an example of a glycosyltransferases in which sialic acid is transferred to oligosaccharides of glycopeptides in the Golgi prior to glycopeptide secretion. In this study, sialyltransferase activities were determined in plasma, liver, and intestinal mucosa following a standardized 25% body surface area thermal injury in the rat. A statistically significant increase in sialyltransferase activity was found in liver and small intestine which were maximal at 24 hours after the injury. These increased sialyltransferase activities were accompanied by a statistically significant 2 to 4 fold elevation in plasma sialyltransferase activity at 24 hours. The plasma and liver elevations in these activities were similar to elevations seen in other models of acute inflammation using turpentine injections and bacterial infections. The increased sialyltransferase activity within the rat intestine was comparable to increases in intestinal sialyltransferase activity following colchicine treatment and may represent a similar mechanism(s).
Ch
S W
SW
Department of Pediatric, Harvard Medical School, Boston, MA.
Carter
E A
EA
Tompkins
R G
RG
Burke
J F
JF
D000818:Animals; D002056:Burns / Q000201:enzymology*; D007421:Intestine, Small / Q000201:enzymology*; D008099:Liver / Q000201:enzymology; D008297:Male; D051381:Rats; D011917:Rats, Inbred Lew; D012794:Sialic Acids / Q000032:analysis; D012799:Sialyltransferases / Q000378:metabolism*; D013997:Time Factors; D000094802:beta-D-Galactoside alpha 2-6-Sialyltransferase
D012794:Sialic Acids; D012799:Sialyltransferases; D000094802:beta-D-Galactoside alpha 2-6-Sialyltransferase