title: Inhibition of phytohemagglutinin (PHA) induced human T-cell colonies by methylprednisolone.
abstract: The mechanisms underlying the inhibition of T-cell proliferation by methylprednisolone (MPS) have been investigated using a T-cell colony assay. Cell fractionation experiments have demonstrated that MPS exerted its effects at the level of both T-cells and monocytes. Thus, monocytes treated with MPS released a soluble factor that had suppressor activity on T-cell proliferation. Moreover, MPS directly blocked the proliferative capacity of T-cells, as demonstrated by the finding that MPS-treated T-cells formed reduced numbers of colonies even under optimal culture conditions and in the presence of exogenous IL-2.
authors.affiliation: Cattedra di Terapia Medica, Universitá di Genova, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Ghio
authors.forename: V
authors.initials: V
authors.lastname: Pistoia
authors.forename: M
authors.initials: M
authors.lastname: Romagnoli
authors.forename: P
authors.initials: P
authors.lastname: D'Elia
authors.forename: G P
authors.initials: GP
authors.lastname: Boccaccio
mesh_terms: D000938:Antigen-Presenting Cells / Q000187:drug effects; D002469:Cell Separation; D002478:Cells, Cultured; D003470:Culture Media; D006801:Humans; D008775:Methylprednisolone / Q000494:pharmacology*; D010835:Phytohemagglutinins / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000187:drug effects*
chemical_list: D003470:Culture Media; D010835:Phytohemagglutinins; D008775:Methylprednisolone