Harman in rat brain, lung and human CSF: effect of alcohol consumption.
A simple, sensitive, HPLC method for the determination of harman has been developed and used to quantitate harman in rat brain (0.41 +/- 0.05 ng/g, n = 7) and rat lung (1.88 +/- 0.55 ng/g, n = 6). The definitive identification of harman in these tissues was accomplished by derivatizing the beta-carboline with pentafluorobenzyl bromide and using gas chromatography-electron capture chemical ionization mass spectrometry. Rats treated acutely with ethanol or subchronically with ethanol in the presence or absence of disulfiram did not have altered harman concentrations. Analysis of control human CSF samples and CSF samples taken from alcoholic patients (n = 8) at the time of intoxicated admission and one week later indicated a predominant absence of harman. These data suggest that harman is unlikely to be formed as a result of ethanol ingestion; its origin and pharmacological significance remain to be determined.
Bosin
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Pharmacology Section, Indiana University School of Medicine, Bloomington 47405.
Borg
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Faull
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KF
D000428:Alcohol Drinking; D000470:Alkaloids / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000378:metabolism*; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid; D004221:Disulfiram / Q000494:pharmacology; D000431:Ethanol / Q000494:pharmacology; D006247:Harmine / Q000031:analogs & derivatives / Q000134:cerebrospinal fluid / Q000378:metabolism*; D006801:Humans; D008168:Lung / Q000378:metabolism*; D008297:Male; D013058:Mass Spectrometry; D051381:Rats; D011919:Rats, Inbred Strains
D000470:Alkaloids; D000431:Ethanol; D006247:Harmine; C005010:harman; D004221:Disulfiram