Sequence specificity of Z-DNA formation in oligonucleotides.
The sequence specific requirement for B----Z transition in solution was examined in d(CGTGCGCACG), d(CGTACGTACG), d(ACGTACGT) in presence of various Z-inducing factors. Conformational studies show that inspite of the alternating nature of purines and pyrimidines, the aforementioned sequences do not undergo B----Z transition under the influence of NaCl, hexamine cobalt chloride and ethanol. A comparison with the crystal structures of an assorted array of purine and pyrimidine sequences show that the sequence requirement for B----Z transition is much more stringent in solution as compared to the solid state. The disruptive influence of AT base pairs in B to Z transition is discussed.
Kadalayil
L P
LP
Molecular Biophysics Unit, Indian Institute of Science, Bangalore.
Majumder
K
K
Mishra
R K
RK
Brahmachari
S K
SK
D001483:Base Sequence; D002942:Circular Dichroism; D004247:DNA; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis*; D011687:Purines / Q000032:analysis; D011743:Pyrimidines / Q000032:analysis; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet
D009838:Oligodeoxyribonucleotides; D011687:Purines; D011743:Pyrimidines; D012996:Solutions; D004247:DNA; D015252:Deoxyribonucleases, Type II Site-Specific; C037130:GCGC-specific type II deoxyribonucleases