The hypothalamic-angiotensin system: location and functional considerations.
Improved immunohistochemical and quantitative microiontophoretic methods were used to characterise angiotensinergic and angiotensin-sensitive neurones in the paraventricular nucleus (PVN) of the rat. The results can be summarised as follows: 1) Angiotensinogen was found in PVN neurones, astrocytes in the diencephalon which make putative contacts with microvessels, and in cells of the choroid plexus. 2) Affinity-purified angiotensin II/III antibodies were used to locate immunoreactive AII/III in large PVN neurones and their fibre tracts which project either caudally or ventrally to the neurohypophysis. 3) Quantitative microiontophoretic studies showed that PVN neurones are more sensitive to angiotensin II than to angiotensin II. 4) Iontophoretic co-application of the selective aminopeptidase inhibitors bestatin and amastatin, together with angiotensin II and angiotensin III produced results consistent with a central role for angiotensin III.
Felix
D
D
Division of Animal Physiology, University of Berne, Switzerland.
Harding
J W
JW
Imboden
H
H
D000626:Aminopeptidases / Q000494:pharmacology; D000804:Angiotensin II / Q000031:analogs & derivatives* / Q000032:analysis* / Q000494:pharmacology / Q000502:physiology; D000805:Angiotensin III / Q000032:analysis* / Q000494:pharmacology / Q000502:physiology; D000818:Animals; D000900:Anti-Bacterial Agents; D007150:Immunohistochemistry; D007478:Iontophoresis; D007930:Leucine / Q000031:analogs & derivatives / Q000494:pharmacology; D008297:Male; D009842:Oligopeptides / Q000494:pharmacology; D010286:Paraventricular Hypothalamic Nucleus / Q000032:analysis* / Q000502:physiology; D010455:Peptides; D051381:Rats; D011918:Rats, Inbred SHR; D011919:Rats, Inbred Strains; D011921:Rats, Inbred WKY
D000900:Anti-Bacterial Agents; D009842:Oligopeptides; D010455:Peptides; D000804:Angiotensin II; D000805:Angiotensin III; C016977:amastatin; D000626:Aminopeptidases; D007930:Leucine; C012211:ubenimex