title: Quantitative analysis of serum methohexital by GLC using capillary column and nitrogen-selective detection.
abstract: A gas chromatographic method for routine quantitation of methohexital in plasma samples is reported. One-step extraction in organic phase, the use of a fused silica capillary column, and nitrogen-selective detection permit simple, precise, and sensitive determination of methohexital in plasma. A linear relationship is described between peak height ratio and methohexital concentrations ranging from 0.125 to 50.0 micrograms/ml (r = 0.998). The sensitivity limit of the assay was 6 ng/ml in plasma. No interfering peak was observed with numerous other drugs. The procedure was successfully applied to the determination of pharmacokinetic parameters of methohexital after IV administration or continuous infusion in a child and an adult.
authors.affiliation: Département de Pharmacologie, Faculté de Médecine, Nantes, France.
authors.forename: Y
authors.initials: Y
authors.lastname: Le Normand
authors.forename: C
authors.initials: C
authors.lastname: De Villepoix
authors.forename: A
authors.initials: A
authors.lastname: Athouel
authors.forename: M F
authors.initials: MF
authors.lastname: Kergueris
authors.forename: M
authors.initials: M
authors.lastname: Bourin
authors.forename: C
authors.initials: C
authors.lastname: Larousse
mesh_terms: D055598:Chemical Phenomena; D002621:Chemistry; D002849:Chromatography, Gas; D006801:Humans; D008723:Methohexital / Q000097:blood* / Q000378:metabolism / Q000493:pharmacokinetics
chemical_list: D008723:Methohexital