Smoking and interleukin-1 activity released from human alveolar macrophages in healthy subjects.
To evaluate the activation of alveolar macrophages from smoking, we studied interleukin-1 (IL-1) activity released from alveolar macrophages in eight healthy smokers, compared to 12 healthy nonsmokers. We used 24-hour culture supernatants containing IL-1 of bronchoalveolar lavage fluid (BALF) macrophages/blood monocytes with or without LPS stimulation. Using C3H/HeJ thymocyte PHA costimulation assay, we found that IL-1 activity released from LPS stimulated BALF macrophages was significantly higher in smokers (2.39 +/- 0.33 U/ml) than in nonsmokers (1.47 +/- 0.19 U/ml, p less than 0.05). We also detected IL-1 inhibitory activity in supernatants by using IL-1 inhibitory assay. The inhibitory activity was higher in nonsmokers than in smokers especially under LPS stimulation. The presence of inhibitory factors other than prostaglandin-E2 was suggested from the differential response to the addition of indomethacin into cultures from nonstimulated and LPS-stimulated supernatants of BALF macrophages.
Nagai
S
S
2nd Department of Medicine, Kyoto University, Japan.
Takeuchi
M
M
Watanabe
K
K
Aung
H
H
Izumi
T
T
D000328:Adult; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D015232:Dinoprostone / Q000494:pharmacology; D005260:Female; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology; D007375:Interleukin-1 / Q000096:biosynthesis* / Q000502:physiology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism; D011650:Pulmonary Alveoli / Q000473:pathology*; D012907:Smoking / Q000009:adverse effects*
D007375:Interleukin-1; D008070:Lipopolysaccharides; D015232:Dinoprostone; D007213:Indomethacin