Tandem mass spectrometry in vitamin E analysis.
Tandem mass spectrometry is applied to the tocopherols and representative tocotrienols of the vitamin E family. The collision-induced dissociation/mass analysed ion kinetic energy spectra generated from three ions in the electron impact ionization spectra of 5,7,8-trimethyltocol, 5,7,8-trimethyltocotrienol, 5,7-, 5,8- and 7,8-dimethyltocol and 7,8-dimethyltocotrienol are described. The technique allowed direct physical characterization of each class of tocochromanol, and in the case of monomethyltocols differentiation of 5-methyltocol from the 7- and 8-methyltocol isomers, and its value in analysis of biological tissue extracts is established.
Walton
T J
TJ
School of Biological Sciences, University College of Swansea, UK.
Mullins
C J
CJ
Newton
R P
RP
Brenton
A G
AG
Beynon
J H
JH
D002855:Chromatography, Thin Layer; D002942:Circular Dichroism; D013058:Mass Spectrometry; D014810:Vitamin E / Q000031:analogs & derivatives / Q000032:analysis*
D014810:Vitamin E