title: Chemotactic activity of chicken blood mononuclear leukocytes from 15I5-B-congenic lines to bacterially-derived chemoattractants.
abstract: The chemotactic activity of chicken blood mononuclear leukocytes was examined in partially-developed 15I5-B-congenic chicken lines using Enterobacter cloacae culture supernatant and formyl-methionyl-leucyl-phenylanine (f-met-leu-phe) (10(-5)M). Cells from seven different coded B-congenic lines were used to study each chemoattractant in vitro. Mononuclear cells from lines .15I-B5,.C-B12 and the background line 15I5 (B15) exhibited a significantly greater directed migration to bacterial supernatant than did cells from four lines carrying the B2, B2, B13, and B19 haplotypes, respectively. Similarly response to f-met-leu-phe was greatest in lines .15I-B5,.C-B12 and .N-B21, with the same four lines exhibiting a significantly lower response. Since f-met-leu-phe was originally isolated from bacteria, the results indicate that these lines possess differential chemotactic responses to certain bacterially-derived chemoattractants. Major histocompatibility complex (MHC) differences between the lines may serve as a genetic basis for the differential responses. Extrapolation of these results to other chemotactic-receptor systems would require further examination.
authors.affiliation: Department of Poultry and Avian Sciences, Cornell University, Ithaca, NY 14853.
authors.forename: M A
authors.initials: MA
authors.lastname: Qureshi
authors.forename: R R
authors.initials: RR
authors.lastname: Dietert
authors.forename: L D
authors.initials: LD
authors.lastname: Bacon
mesh_terms: D000818:Animals; D002630:Chemotactic Factors / Q000276:immunology; D002634:Chemotaxis, Leukocyte; D002645:Chickens / Q000276:immunology*; D004754:Enterobacter / Q000276:immunology; D066298:In Vitro Techniques; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D013045:Species Specificity
chemical_list: D002630:Chemotactic Factors