Radioimmunoassay of total and free corticosterone in rat plasma: measurement of the effect of different doses of corticosterone.
A radioimmunological method was developed for determining total and free corticosterone in rat plasma. This method was used to determine the dose-response curve of corticosterone and to measure the elimination and study the half-lives of total and free corticosterone in rat plasma with a dose of 5 mg/kg. The elimination with a dose of 5 mg/kg, when drawn on the half-logarithmic scale, formed a straight line. The half-lives for total and free corticosterone were 25 and 15 min, respectively.
Sainio
E L
EL
Department of Pharmacology and Toxicology, University of Kuopio, Finland.
Lehtola
T
T
Roininen
P
P
D000818:Animals; D001665:Binding Sites; D003345:Corticosterone / Q000097:blood* / Q000493:pharmacokinetics; D003429:Cross Reactions; D004305:Dose-Response Relationship, Drug; D006207:Half-Life; D008297:Male; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains
D003345:Corticosterone