The secondary in vitro murine cytotoxic T cell response to the flavivirus, West Nile.
A secondary in vitro murine cytotoxic response to the flavivirus, West Nile (WNV) is described. Cytotoxic activity was obtained from spleen cells of mice primed 7 days previously with 10(6) p.f.u. WNV and boosted in vitro for a further 5 days with WNV-infected stimulator spleen cells. The cells responsible for lysis of WNV-infected target cells were restricted by class 1 H-2 antigens. The K region of the H-2k haplotype and both the K and D regions of the H-2d haplotype were permissive. The cytotoxic cells were virus-specific with respect to WNV and Influenza. The phenotype of the cells which mediated cytotoxicity was Thy 1+, Lyt 2+ and L3T4-; however an L3T4+ helper population was required for the optimal generation of the cytotoxic response in vitro.
Kesson
A M
AM
Department of Microbiology, John Curtin School of Medical Research, Canberra, A.C.T., Australia.
Blanden
R V
RV
Mullbacher
A
A
D000818:Animals; D003602:Cytotoxicity, Immunologic; D005260:Female; D006183:H-2 Antigens / Q000235:genetics / Q000276:immunology; D006239:Haplotypes; D007117:Immunization, Secondary; D007125:Immunogenetics; D066298:In Vitro Techniques; D051379:Mice; D008815:Mice, Inbred Strains; D010641:Phenotype; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014902:West Nile virus / Q000276:immunology*
D006183:H-2 Antigens