Bioluminescent microassay of various metabolites using bacterial luciferase co-immobilized with multienzyme systems.
Co-immobilization methods have been developed for a bienzymatic system of luminescent Beneckea harveyi bacteria with formate dehydrogenase, glucose-6-phosphate dehydrogenase, and phosphoglucomutase. Bioluminescent assays have been devised for NADH, NAD, FMN, glucose 6-phosphate, and glucose 1-phosphate using the co-immobilized enzyme preparation. The lowest detection limits were in the picomole range with the bacterial extract and in the femtomole range with the partially purified enzymes, bacterial luciferase, and NADH:FMN oxidoreductase.
Ugarova
N N
NN
Chemistry Department, Lomonosov Moscow State University, USSR.
Lebedeva
O V
OV
Frumkina
I G
IG
D004800:Enzymes, Immobilized; D038181:FMN Reductase; D005486:Flavin Mononucleotide / Q000032:analysis; D019298:Glucose-6-Phosphate; D005958:Glucosephosphates / Q000032:analysis; D008156:Luciferases; D008163:Luminescent Measurements; D009097:Multienzyme Complexes; D009243:NAD / Q000032:analysis; D009247:NADH, NADPH Oxidoreductases; D014733:Vibrio / Q000201:enzymology*; D014737:Vibrionaceae / Q000201:enzymology*
D004800:Enzymes, Immobilized; D005958:Glucosephosphates; D009097:Multienzyme Complexes; D009243:NAD; D019298:Glucose-6-Phosphate; D005486:Flavin Mononucleotide; C031590:glucose-1-phosphate; D008156:Luciferases; D038181:FMN Reductase; D009247:NADH, NADPH Oxidoreductases