An ELISA method for detecting antibodies to the T cell antigen receptor.
An ELISA method for the detection of monoclonal antibodies (MAb) to the T3-T cell antigen receptor (TCR) complex was devised. The T3-TCR complex was solubilised using digitonin and a rat anti-T3 MAb (Campath 3) was used to bind it to an ELISA plate. Normal rat serum was used to block cross-reactivity between the rat MAb and peroxidase-conjugated rabbit anti-mouse immunoglobulins. The assay was tested on four T cell tumour lines and successfully detected MAbs to TCR beta chain variable regions, as well as the anti-T3 MAb UCHT1. Other anti-T3 MAbs were not detected because Campath 3 blocked their binding. None of a panel of MAbs reacting with other T cell surface antigens reacted in the assay.
Clark
D M
DM
Department of Experimental Pathology, St. Mary's Hospital Medical School, London, U.K.
Boylston
A W
AW
D000911:Antibodies, Monoclonal / Q000032:analysis*; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology; D001666:Binding Sites, Antibody; D002460:Cell Line; D003902:Detergents; D004072:Digitonin; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D011948:Receptors, Antigen, T-Cell / Q000276:immunology*; D012995:Solubility; D013347:Subcellular Fractions / Q000276:immunology
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D003902:Detergents; D011948:Receptors, Antigen, T-Cell; D004072:Digitonin