title: Enhancing effect of anti-HLA class I monoclonal antibodies on T cell proliferation induced via CD2 molecule.
abstract: The mAb 131 to a determinant preferentially expressed on the gene products of the HLA-A locus, the mAb Q6/64 and 4E to determinants preferentially expressed on the gene products of the HLA-B locus, the anti-HLA-A2,A28 mAb CR11-351, HO-2, HO-3, HO-4, and KS1, and the anti-HLA-B7 cross-reacting group mAb KS4 enhanced proliferation of T cells in most, if not all, the PBMC preparations stimulated with the anti-CD2 mAb 9-1 + 9.6. The mAb CR10-215, W6/32, and 6/31 to distinct monomorphic determinants of HLA class I antigens enhanced CD2-induced T cell proliferation in at most 30% of the PBMC preparations tested. The anti human beta 2-microglobulin (beta 2-mu) mAb NAMB-1 displayed no detectable effect on the proliferation of T cells stimulated with the mAb 9-1 + 9.6. The enhancing effect of anti-HLA class I mAb is specific, is dose dependent, is not abrogated by the addition of exogenous IL-1 and IL-2 to the cultures, and reflects the interaction of anti-HLA class I mAb with T cells. Enhancement of CD2 mediated proliferation of T cells is not a unique property of anti-HLA class I mAb, since the anti-HLA class II mAb Q5/6 and Q5/13 also had a similar effect. Analysis of the kinetics of the enhancing effect of anti-HLA class I mAb suggests that they modulate an early event of T cell activation and may affect the interaction of T cells with mAb 9-1. Phenotyping of T lymphocytes activated by mAb 9-1 + 9.6 in the presence of anti-HLA class I mAb suggests that the enhancing effect of anti-HLA class I mAb may reflect the recruitment of a higher percentage of T cells. The present study has shown for the first time that certain, but not all, the determinants of the HLA class I molecular complex are involved in the proliferation of T cells stimulated with the anti-CD2 mAb 9-1 + 9.6. Furthermore, the inhibitory effect of mAb CR11-351, KS1, Q6/64, and W6/32 on the proliferation of T cells stimulated with mAb OKT3 or with mAb BMA 031 indicates that the same determinants of HLA class I antigens play a differential regulatory role in T cell proliferation induced via the CD2 and CD3 pathway.
authors.affiliation: Istituto di Scienze Biochimiche, II Facolta di Medicina e Chirurgia, Napoli, Italy.
authors.forename: M C
authors.initials: MC
authors.lastname: Turco
authors.forename: M
authors.initials: M
authors.lastname: de Felice
authors.forename: L
authors.initials: L
authors.lastname: Corbo
authors.forename: P C
authors.initials: PC
authors.lastname: Giarrusso
authors.forename: S Y
authors.initials: SY
authors.lastname: Yang
authors.forename: S
authors.initials: S
authors.lastname: Ferrone
authors.forename: S
authors.initials: S
authors.lastname: Venuta
mesh_terms: D000276:Adjuvants, Immunologic / Q000502:physiology*; D000911:Antibodies, Monoclonal / Q000502:physiology*; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology*; D003470:Culture Media / Q000032:analysis; D006680:HLA Antigens / Q000276:immunology*; D006681:HLA-D Antigens / Q000276:immunology; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000502:physiology; D007376:Interleukin-2 / Q000032:analysis; D007700:Kinetics; D008213:Lymphocyte Activation; D015375:Receptors, Interleukin-2 / Q000032:analysis; D011990:Receptors, Transferrin / Q000032:analysis; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D003470:Culture Media; D006680:HLA Antigens; D006681:HLA-D Antigens; D007140:Immunoglobulin Fab Fragments; D007376:Interleukin-2; D015375:Receptors, Interleukin-2; D011990:Receptors, Transferrin