title: [Role of the cytosolic factor in the interaction of [3H] alpha-tocopherol with rat liver nuclei].
abstract: A protein fraction (Mr = 30-70 kD) specifically binding [3H]alpha-tocopherol was isolated from rat liver cytosol. Using high performance ion exchange chromatography, this fraction was separated into acid and alkaline protein subfractions. Acid proteins make up to 41% of the total protein pool and they bind the label 8 times more intensively than the alkaline ones. Cytosol and its protein fraction with an average molecular mass increase 2.2-2.5-fold the binding of labeled vitamin E to isolated liver nuclei. It is concluded that the cytosolic proteins having a medium molecular mass are involved in tocopherol interaction with the nuclei.
authors.forename: G V
authors.initials: GV
authors.lastname: Donchenko
authors.forename: L B
authors.initials: LB
authors.lastname: Malen'kikh
authors.forename: A A
authors.initials: AA
authors.lastname: Kapralov
authors.forename: O Ia
authors.initials: OIa
authors.lastname: Baryk
authors.forename: V N
authors.initials: VN
authors.lastname: Mel'nik
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism*; D005260:Female; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D014810:Vitamin E / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D002352:Carrier Proteins; D014810:Vitamin E
vernacular_title: Uchastie tsitozol'nogo faktory vo vzaimodeĭstvii [3H]alpha-tokoferola s iadrami pecheni krys.