Comparison between standard ELISA and dot-ELISA for serodiagnosis of human trichinosis.
The dot enzyme-linked immunosorbent assay (dot-ELISA) provided rapid and accurate detection of human trichinosis. Comparable sensitivity and specificity of the standard ELISA and dot-ELISA were obtained when sera of 18 confirmed cases of trichinosis were screened. Cross-reactivity was assayed with sera from 31 Chinese subjects with other parasitic infections. Only 2 cross-reactions occurred in the dot-ELISA, compared with 7 in the standard ELISA.
Chan
S W
SW
Department of Zoology, University of Hong Kong.
Ko
R C
RC
D000947:Antigens, Helminth / Q000032:analysis; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D014235:Trichinellosis / Q000175:diagnosis*
D000947:Antigens, Helminth