Gas chromatographic determination of guanadrel in plasma and urine.
To evaluate the pharmacokinetics and drug availability from various dosage formulations, a method for the determination of guanadrel, (1,4-dioxaspiro[4,5]dec-2-ylmethyl)guanidine, in plasma and urine was required. A gas chromatographic procedure, based on formation of a hexafluoroacetylacetone derivative in a two-phase system of water and toluene, was developed. The limit of determination of the method is 5 ng/ml guanadrel in plasma and 15 ng/ml guanadrel in urine. Statistical analyses indicate average recoveries of 98.1 +/- 18.0 and 104.4 +/- 15.6% from plasma and urine, respectively. Mass spectrometric analyses, in conjunction with gas chromatography, confirmed the specificity of the method for intact drug. The procedure was applied successfully to drug absorption studies in humans.
Kaiser
D G
DG
Drug Metabolism Research, Upjohn Company, Kalamazoo 49001.
Vangiessen
G J
GJ
Shah
J A
JA
Weber
D J
DJ
D055598:Chemical Phenomena; D002621:Chemistry; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D006146:Guanidines / Q000097:blood / Q000378:metabolism* / Q000652:urine; D006801:Humans; D008297:Male; D013696:Temperature; D013997:Time Factors
D006146:Guanidines; C004945:guanadrel