Anti-idiotypic induced suppression of Sjögren's syndrome associated anti-La autoantibody secretion in vitro.
Peripheral blood mononuclear cells from patients with Sjögren's Syndrome spontaneously secrete autoantibodies to the La antigen when cultured in vitro. This paper reports that specific IgG autoantibody production in vitro is suppressed by pre-treatment of CD8+ enriched T cells with rabbit polyclonal antibodies to idiotypes borne by circulating autologous anti-La antibodies. Treatment of this T cell subpopulation with anti-idiotypes specific for circulating anti-La antibodies from other patients or for anti-DNA antibodies was without effect on anti-La antibody production. Similarly anti-La anti-idiotypes had no effect on the production of autoantibodies to other ribonucleoprotein antigens such as nRNP/Sm. These data show that CD8+ T cells are the main targets for anti-idiotypic control in vitro. We suggest that the relative deficit of these cells, plus a surfeit of CD4+ T cells at the site of the pathological lesion within the salivary gland permits localized production of autoantibodies. Thus, dysregulation of the idiotypic network could contribute to the pathogenesis of Sjögren's Syndrome.
Horsfall
A C
AC
Division of Clinical Immunology, Kennedy Institute of Rheumatology, London, UK.
Mumford
P A
PA
Venables
P J
PJ
Maini
R N
RN
D000974:Antibodies, Antinuclear / Q000096:biosynthesis*; D001324:Autoantigens / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology; D006801:Humans; D007108:Immune Tolerance; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D012261:Ribonucleoproteins; D012859:Sjogren's Syndrome / Q000276:immunology*; D013601:T-Lymphocytes / Q000145:classification / Q000276:immunology; D000097585:SS-B Antigen
D000974:Antibodies, Antinuclear; D001324:Autoantigens; D007130:Immunoglobulin Idiotypes; D012261:Ribonucleoproteins; D000097585:SS-B Antigen