Effect of vitamin A deficiency on pulmonary defense systems of guinea pig lung.
Vitamin A deficiency causes a significant decrease in superoxide dismutase, glutathione peroxidase and GSH levels. Simultaneously, it causes a marked increase in Phase I microsomal oxidation (cytochrome P-450, aniline hydroxylase, PNA-O-demethylase) as well as in enzymatic and non-enzymatic lipid peroxidation in lung. Among Phase II enzymes, cytosolic chlorodinitrobenzene glutathione-S-transferase (CDNB-GST) activity showed a significant increase whereas microsomal UDP-glucuronyl transferase and cytosolic p-nitrobenzoyl chloride- and dichloro nitrobenzyl chloride-S-transferase activities showed variable decrease reflecting an imbalance in the Phase I and Phase II enzyme systems. CDNB-GST and non-Se-GSH-Px, registered a parallel rise with pulmonary cytochrome P-450, aniline hydroxylase and O-demethylase, as an adaptive response to elevated Phase I enzyme activity.
Nair
C R
CR
Department of Biochemistry, Meharry Medical College, Nashville, Tennessee 37208.
Davis
M M
MM
Das
S K
SK
D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000032:analysis; D005978:Glutathione / Q000032:analysis; D005979:Glutathione Peroxidase / Q000032:analysis; D005982:Glutathione Transferase / Q000032:analysis; D006168:Guinea Pigs; D066298:In Vitro Techniques; D008168:Lung / Q000201:enzymology / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000032:analysis; D014802:Vitamin A Deficiency / Q000378:metabolism*
D003577:Cytochrome P-450 Enzyme System; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005982:Glutathione Transferase; D005978:Glutathione