Effect of Nocardia rubra cell wall skeleton on interleukin 1 production from mouse peritoneal macrophages.
Nocardia rubra cell wall skeleton (N-CWS) was shown to augment interleukin 1 (IL-1) production from peritoneal resident and exudate macrophages in C3H/HeN mice. The mol. wt of the N-CWS-induced IL-1 product was about 17,000 daltons, which is a similar weight to that obtained by lipopolysaccharide stimulation. The stimulation of IL-1 production by N-CWS was seen as early as 8 h after the start of incubation and peak production was observed at 48 h. Profound effects were seen with 10 micrograms/ml or more of N-CWS. Experiments on the regulation of the N-CWS-augmented IL-1 production showed that prostaglandin E2 inhibited the augmentation, and indomethacin (cyclo-oxygenase inhibitor) further augmented it. Leukotriene B4 and AA861 (lipoxygenase inhibitor) had no effect. Our findings suggest that the previously reported adjuvant effect of N-CWS may, in part, be mediated via its ability to stimulate IL-1 production; and that such a stimulation may be blocked by prostaglandins.
Inamura
N
N
Exploratory Research Laboratories, Fujisawa Pharmaceutical Co. Ltd., Ibaraki, Japan.
Nakahara
K
K
Kuroda
Y
Y
Yamaguchi
I
I
Aoki
H
H
Kohsaka
M
M
D000818:Animals; D002473:Cell Wall / Q000276:immunology; D015232:Dinoprostone / Q000494:pharmacology; D004306:Dose-Response Relationship, Immunologic; D066298:In Vitro Techniques; D007213:Indomethacin / Q000494:pharmacology; D007375:Interleukin-1 / Q000096:biosynthesis*; D007700:Kinetics; D008264:Macrophages / Q000276:immunology*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D009615:Nocardia / Q000276:immunology*; D010529:Peritoneal Cavity / Q000166:cytology / Q000276:immunology
D007375:Interleukin-1; D015232:Dinoprostone; D007213:Indomethacin