cDNA clones coding for the complete murine B chain of complement C1q: nucleotide and derived amino acid sequences.
We have isolated cDNA clones covering the complete B chain of the complement subunit C1q from mouse; this subunit initiates the classical complement pathway. Deoxynucleotide sequence analysis shows that these clones contain 156 nucleotides of the 5' untranslated region, followed by sequences coding for the 25 amino acids of the signal peptide, all of the 228 amino acids of the mature protein and 140 nucleotides of the 3' untranslated region, including a poly A addition signal. The coding region for the mature protein contains 261 nucleotides for the Gly-X-Y repeat and 408 nucleotides for the globular portion of C1q. By comparing the nucleotide sequence of the mouse B chain of C1q with the B chain from human (Reid, K. B. M., 1985, Biochem. J. 231, 729), we find a high homology (80%) within the mature protein, a lower homology within the signal peptide (59%) and the 3' untranslated region (47%) and no homology (26%) in the 5' untranslated region.
Wood
L
L
Molecular Biology Research, Upjohn Company, Kalamazoo, Michigan 49001.
Pulaski
S
S
Vogeli
G
G
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003166:Complement Activating Enzymes / Q000235:genetics*; D003172:Complement C1 / Q000235:genetics*; D015922:Complement C1q; D004247:DNA / Q000235:genetics*; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D021382:Protein Sorting Signals / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
D003172:Complement C1; D021382:Protein Sorting Signals; D015922:Complement C1q; D004247:DNA; D003166:Complement Activating Enzymes