Chemotactic response of vascular smooth muscle cells to acetoacetylated low-density lipoprotein.
We studied the effect of acetoacetylated low-density lipoprotein (LDL), which is recognized by the scavenger receptor, on the migration of fetal bovine aortic smooth muscle cells in culture, using a Neuro Probe 48-well microchemotaxis chamber. Acetoacetylated LDL is chemotactic and chemokinetic for the smooth muscle cells, and the effect is maximal with 50 micrograms/ml of protein, while native LDL has no significant chemotactic activity. These results suggest that denatured LDL might play an important role in the recruitment of smooth muscle cells from the media into the intima in atherosclerosis.
Naito
M
M
Department of Geriatrics, Nagoya University School of Medicine, Nagoya, Japan.
Hayashi
T
T
Funaki
C
C
Kuzuya
M
M
Asai
K
K
Kuzuya
F
F
D000818:Animals; D001161:Arteriosclerosis / Q000473:pathology*; D002417:Cattle; D002465:Cell Movement; D002478:Cells, Cultured; D002633:Chemotaxis / Q000187:drug effects; D008077:Lipoproteins, LDL / Q000494:pharmacology*; D009131:Muscle, Smooth, Vascular / Q000166:cytology*
D008077:Lipoproteins, LDL