title: Epidermal growth factor stimulates luteinizing hormone and arachidonic acid release in rat pituitary cells.
abstract: Epidermal growth factor (EGF) directly enhanced luteinizing hormone (LH) release from dispersed rat pituitary cells in monolayer cultures as well as in superfusion columns. This 2.3-fold stimulatory effect was dose and time dependent and was also reconfirmed in a superfusion system. Retinal, a protein kinase C inhibitor, counteracted the EGF effect only partially. Further experiments were therefore carried out to investigate alternate EGF mechanisms. Nordihydroguaiaretic acid and chloroquine suppressed the stimulatory effect of EGF in a dose-dependent manner. Moreover, EGF (10(-7) M) stimulated [3H]arachidonate release from pre-labelled rat pituitary cells. This indicates that phospholipase A2 and arachidonic acid may be involved in EGF action on LH release from rat pituicytes.
authors.affiliation: Department of Obstetrics and Gynaecology, University of Heidelberg, F.R.G.
authors.forename: A
authors.initials: A
authors.lastname: Przylipiak
authors.forename: L
authors.initials: L
authors.lastname: Kiesel
authors.forename: T
authors.initials: T
authors.lastname: Rabe
authors.forename: K
authors.initials: K
authors.lastname: Helm
authors.forename: M
authors.initials: M
authors.lastname: Przylipiak
authors.forename: B
authors.initials: B
authors.lastname: Runnebaum
mesh_terms: D000818:Animals; D001095:Arachidonic Acids / Q000378:metabolism*; D002478:Cells, Cultured; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D005260:Female; D007986:Luteinizing Hormone / Q000378:metabolism*; D010902:Pituitary Gland / Q000166:cytology / Q000378:metabolism*; D011493:Protein Kinase C / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D001095:Arachidonic Acids; D004815:Epidermal Growth Factor; D007986:Luteinizing Hormone; D011493:Protein Kinase C