Induction of persistent ovarian follicular structures following administration of progesterone near the onset of estrus in dairy cattle.
The objective of this study was to determine if progesterone administered near the onset of estrus in dairy cows would block the preovulatory surge of LH and result in subsequent persistence of ovarian follicular structures. Following synchronization of estrus with prostaglandin F2 alpha, 20 multiparous, non-lactating Holstein cows were randomly assigned into three groups: 1 ml ethanol administered i.m. at 12-h intervals for 24 h (n = 6; group 1); 1 mg progesterone administered i.m. at 12-h intervals for 24 h (n = 7; group 2); 2.5 mg progesterone administered i.v. at the onset of standing estrus (n = 7; group 3). Ovarian structures were palpated per rectum on the day of estrus and twice weekly for 14 d. Blood was collected every 2 h from onset of standing estrus for 30 h, and concentrations of LH and progesterone were determined. Numbers of cows diagnosed with persistent follicles 10 d after estrus were 1 of 6 (group 1), 2 of 7 (group 2), and 5 of 7 (group 3). The preovulatory surge of LH did not occur during the sampling period (30 h) for 1 of 6, 7 of 7 and 5 of 7 cows, for groups 1, 2, and 3, respectively, and mean serum concentrations of LH were higher in group 1 than in groups 2 and 3. Serum concentration of progesterone (ng/ml) was higher in group 1 (1.9 +/- .4) than in groups 2 (.9 +/- .4) and 3 (.9 +/- .4) 10 d following estrus. Blocking the preovulatory surge of LH with exogenous progesterone resulted in persistence of ovarian follicles.
Lee
C N
CN
Department of Dairy Science, University of Missouri, Columbia 65211.
Cook
D L
DL
Parfet
J R
JR
Smith
C A
CA
Youngquist
R S
RS
Garverick
H A
HA
D000818:Animals; D002417:Cattle / Q000502:physiology*; D004971:Estrus / Q000187:drug effects*; D005260:Female; D005498:Follicular Phase / Q000187:drug effects*; D007986:Luteinizing Hormone / Q000097:blood / Q000378:metabolism*; D006080:Ovarian Follicle / Q000187:drug effects*; D011374:Progesterone / Q000494:pharmacology*
D011374:Progesterone; D007986:Luteinizing Hormone