Introduction of a porcine growth hormone fusion gene into transgenic pigs promotes growth.
Six transgenic pigs have been produced by microinjecting a human metallothionein promoter/porcine growth hormone gene construct into the pronuclei of fertilized eggs which were transferred to synchronized recipient sows. The resulting transgenic animals contained between 0.5 and 15 copies of the gene construct per cell, and at least one of the animals expressed the introduced gene and grew at an increased rate compared to both transgenic and non-transgenic littermates. Some of the transgenic animals that did not appear to grow at increased rates were found to contain rearranged gene sequences. Two of the transgenic pigs have been shown to pass on the introduced genes to their offspring.
Vize
P D
PD
Department of Biochemistry, University of Adelaide, Australia.
Michalska
A E
AE
Ashman
R
R
Lloyd
B
B
Stone
B A
BA
Quinn
P
P
Wells
J R
JR
Seamark
R F
RF
D000818:Animals; D030801:Animals, Genetically Modified / Q000254:growth & development*; D003001:Cloning, Molecular; D005260:Female; D015321:Gene Rearrangement; D013006:Growth Hormone / Q000235:genetics*; D013552:Swine / Q000254:growth & development*
D013006:Growth Hormone