[Controlled search for the producers of ionophore antibiotics among Streptomycetes].
A procedure was developed for directed screening of cultures producing ionophore antibiotics among streptomycetes. The procedure is based on measuring the membrane potential generated in the presence of the Men+/nH+ = -expchanger-protonophore couple. It provided isolation of cultures producing ionophore antibiotics at the fermentation broth stage. It was possible to use the procedure in screening both electrogenic and nonelectrogenic ionophores and to rapidly differentiate them. 5 cultures producing ionophore antibiotics were detected with this procedure; 3 of them carry out nonelectrogenic transport of the cations. The cation transport in the other two cultures was electrogenic. Cation selectivity of the antibiotics produced by the cultures was determined with the procedure. An antibiotic identical to indanomycin was isolated from the culture fluid of Streptomyces chromogenes.
Drobysheva
T N
TN
Korobkova
T P
TP
Antonenko
Iu N
IuN
Singan
E M
EM
Ivanitskaia
L P
LP
D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D003470:Culture Media; D004345:Drug Industry / Q000379:methods*; D066298:In Vitro Techniques; D007218:Industrial Microbiology / Q000379:methods*; D007476:Ionophores; D013302:Streptomyces / Q000254:growth & development / Q000378:metabolism*; D014586:USSR
D000900:Anti-Bacterial Agents; D003470:Culture Media; D007476:Ionophores
Naprablennyĭ poisk produtsentov antibiotikov-ionoforov iz streptomitsetov.