2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD)-induced alterations in lipid peroxidation, enzymes, and divalent cations in rat testis.
1. 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) produces atrophy, morphological changes, impaired spermatogenesis, and epididymal lesions in testis of experimental animals. The effects of TCDD administration to male rats on various parameters in the testes were examined. 2. Nine days after TCDD administration, significant decreases in body and testes weights occurred. However, the testes weight as a percent of body weight was higher in treated than control animals. 3. An increase in lipid peroxidation (content of thiobarbituric acid reactive substances) occurred in conjunction with the decrease in testicular weights. 4. TCDD administration produced a 3-fold increase in protein kinase C activity, small but significant decrease is superoxide dismutase and glutathione peroxidase activities, and no effect on catalase, glutathione reductase or glutathione S-transferase activities in the testes. 5. Nine days after treatment with TCDD, in the testes the iron content of whole tissue and cytosol increased while a decrease in microsomal iron was observed. The copper content of mitochondria and microsomes decreased with a corresponding increase in cytosol copper content. A small increase in the zinc content of whole testes occurred. 6. The data indicate that testicular atrophy due to TCDD may be associated with lipid mobilization and peroxidation.
al-Bayati
Z A
ZA
Department of Pharmaceutical Sciences, College of Pharmacy, University of Nebraska Medical Center, Omaha, NE 68105-1065.
Wahba
Z Z
ZZ
Stohs
S J
SJ
D000818:Animals; D003300:Copper / Q000378:metabolism; D004147:Dioxins / Q000633:toxicity*; D007501:Iron / Q000378:metabolism; D008054:Lipid Peroxides / Q000378:metabolism*; D008297:Male; D008861:Microsomes / Q000187:drug effects / Q000378:metabolism; D009929:Organ Size / Q000187:drug effects; D000072317:Polychlorinated Dibenzodioxins / Q000633:toxicity*; D011493:Protein Kinase C / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013737:Testis / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D013858:Thiobarbiturates / Q000378:metabolism; D015032:Zinc / Q000378:metabolism
D004147:Dioxins; D008054:Lipid Peroxides; D000072317:Polychlorinated Dibenzodioxins; D013858:Thiobarbiturates; D003300:Copper; D007501:Iron; D011493:Protein Kinase C; D015032:Zinc; C029684:thiobarbituric acid