A simple isolation procedure for functionally pure components of the bovine alternative complement pathway (ACP) C3 convertase and bovine conglutinin (K).
A simple multicomponent isolation procedure for bovine C3, factor B, factor D and conglutinin (K) from a single serum sample is described. The components of the alternative pathway C3 convertase were isolated in milligram quantities from 800 ml bovine serum and were found to be functionally pure with respect to each other and to factors H and I.
Phipps
D J
DJ
Department of Microbiology and Immunology, Queen's University, Kingston, Ont., Canada.
Aston
W P
WP
D000818:Animals; D002417:Cattle / Q000276:immunology*; D002845:Chromatography; D037222:Collectins; D003166:Complement Activating Enzymes / Q000302:isolation & purification*; D003167:Complement Activation; D003176:Complement C3 / Q000302:isolation & purification; D050577:Complement C3-C5 Convertases / Q000302:isolation & purification*; D011415:Complement Factor B / Q000302:isolation & purification; D011416:Complement Factor D / Q000302:isolation & purification; D003168:Complement Fixation Tests; D003170:Complement Pathway, Alternative; D012712:Serum Globulins / Q000302:isolation & purification
D037222:Collectins; D003176:Complement C3; D012712:Serum Globulins; C024014:conglutinin; D003166:Complement Activating Enzymes; D050577:Complement C3-C5 Convertases; D011416:Complement Factor D; D011415:Complement Factor B