Membrane immunoglobulin: anti-immunoglobulin interactions mediate the phosphorylation of actin associated proteins in the B-lymphocyte.
The binding of membrane immunoglobulin (mIg) by anti-Ig antibodies is known to initiate a mitogenic signal in B lymphocytes. Because in many instances growth control appears to be correlated with phosphokinase activity, as well as with alterations in cytoskeletal architecture, we asked the question whether antibodies binding to mIg would also lead to the specific phosphorylation of lymphocyte actin-associated proteins. Utilizing a myosin affinity technique, we directly examined phosphoproteins that were associated with actin in the chicken B cell. We found that in a few instances the level of phosphorylation was indeed modulated by mIg:anti-Ig interactions. These actin-binding phosphoproteins may be important control elements in the lymphocyte cytoskeleton.
Rosenspire
A J
AJ
Department of Biological Sciences, Wayne State University, Detroit, MI 48202.
Choi
Y S
YS
D000199:Actins / Q000378:metabolism*; D000818:Animals; D000906:Antibodies / Q000502:physiology*; D001402:B-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D002060:Bursa of Fabricius / Q000166:cytology; D002462:Cell Membrane / Q000276:immunology*; D002645:Chickens; D002846:Chromatography, Affinity; D003598:Cytoskeletal Proteins / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D007136:Immunoglobulins / Q000276:immunology* / Q000502:physiology; D008715:Methionine / Q000378:metabolism; D009218:Myosins / Q000378:metabolism; D010710:Phosphates / Q000378:metabolism; D010750:Phosphoproteins / Q000378:metabolism*; D010766:Phosphorylation; D011947:Receptors, Antigen, B-Cell / Q000502:physiology
D000199:Actins; D000906:Antibodies; D003598:Cytoskeletal Proteins; D007136:Immunoglobulins; D010710:Phosphates; D010750:Phosphoproteins; D011947:Receptors, Antigen, B-Cell; D008715:Methionine; D009218:Myosins