title: Assay for hydralazine as its stable p-nitrobenzaldehyde hydrazone.
abstract: A new method of analysis for the antihypertensive drug, hydralazine, is introduced. The assay involves the addition of p-nitrobenzaldehyde to blood samples containing hydralazine, to form a stable Schiff's base, hydralazine p-nitrobenzaldehyde hydrazone. The derivative is extracted from the blood into hexane and the samples are dried under a nitrogen stream. The extracts are then dissolved in mobile phase and analyzed using high-performance liquid chromatography. The extracted samples can be stored for at least 7 days at room temperature or at -20 degrees C. The sensitivity of the assay is better than 300 pg/ml using 3-ml blood samples, and the range can extend to 640 ng/ml. The stability of the extracted samples plus the sensitivity and simplicity of the assay are the main advantages of the method over other selective methods for hydralazine.
authors.affiliation: Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, Canada.
authors.forename: H A
authors.initials: HA
authors.lastname: Semple
authors.forename: Y K
authors.initials: YK
authors.lastname: Tam
authors.forename: S
authors.initials: S
authors.lastname: Tin
authors.forename: R T
authors.initials: RT
authors.lastname: Coutts
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004285:Dogs; D006801:Humans; D006830:Hydralazine / Q000032:analysis* / Q000097:blood; D007202:Indicators and Reagents; D013045:Species Specificity
chemical_list: D007202:Indicators and Reagents; D006830:Hydralazine