title: Application of high-performance liquid chromatography to the analysis of propionyl-L-carnitine by a stereospecific enzyme assay.
abstract: Propionyl-L-carnitine was converted by chemical hydrolysis (0.3 M potassium hydroxide, pH 12.8, room temperature) into L-carnitine, which competes with crotonoylbetaine formation and can be quantitatively evaluated by an enzyme assay. Under the conditions selected, hydrolysis of propionyl-L-carnitine to L-carnitine was completed in a few minutes with less than 0.5% of crotonoylbetaine being formed. The method described is enantioselective and possesses the analytical requirements for assaying propionyl-L-carnitine during chemical synthesis procedures and in pharmaceutical formulations.
authors.affiliation: Real srl, Como, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Marzo
authors.forename: N
authors.initials: N
authors.lastname: Monti
authors.forename: M
authors.initials: M
authors.lastname: Ripamonti
authors.forename: E
authors.initials: E
authors.lastname: Arrigoni Martelli
mesh_terms: D000818:Animals; D002331:Carnitine / Q000031:analogs & derivatives* / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D004285:Dogs; D006801:Humans; D006868:Hydrolysis; D051381:Rats; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism
chemical_list: C003223:propionylcarnitine; D002331:Carnitine