title: The homology between the serum proteins PO2 in pig, Xk in horse and alpha 1B-glycoprotein in human.
abstract: 1. Pig serum Po2 protein and horse Xk protein were purified by FPLC, non-denaturing 2D agarose-PAGE and 2D IPG-PAGE. 2. The separated fractions were electroblotted to poly(4-vinyl-N-methylpyridinium iodide) coated GF/C glass fiber sheets. 3. The partial amino acid sequences and amino acid compositions of different genetic variants of the proteins were determined. 4. The results proved that previously reported polymorphic serum post-albumins in each of these species were homologous to human plasma alpha 1B-glycoprotein.
authors.affiliation: Department of Animal Genetics, State University of Ghent, Merelbeke, Belgium.
authors.forename: A
authors.initials: A
authors.lastname: Van de Weghe
authors.forename: W
authors.initials: W
authors.lastname: Coppieters
authors.forename: G
authors.initials: G
authors.lastname: Bauw
authors.forename: J
authors.initials: J
authors.lastname: Vandekerckhove
authors.forename: Y
authors.initials: Y
authors.lastname: Bouquet
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001798:Blood Proteins / Q000235:genetics* / Q000302:isolation & purification; D006023:Glycoproteins; D006736:Horses; D006801:Humans; D007136:Immunoglobulins; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D013552:Swine
chemical_list: C048566:A1BG protein, human; D000596:Amino Acids; D001798:Blood Proteins; D006023:Glycoproteins; D007136:Immunoglobulins