A new cell line, GS, derived from a human renal cell carcinoma.
A new cell line, GS, of human renal cell carcinoma was established and characterized. It was derived from a metastasis of a human renal cell carcinoma, which appeared 6 years after nephrectomy. The GS cells exhibit basic characteristics of renal cell carcinoma: epithelial cell character, PAS and glycogen positivity, typical ultrastructural features. The cells have a pseudotriploid stemline with a modal number of 75 chromosomes and two marker chromosomes. GS cells formed neither colonies in soft agar nor transplantable tumours in nude mice but produced a factor(s) stimulating growth and colony forming activity of indicator cells.
Vydra
J
J
Oncological Department, Therapeutical Institute Ples.
Vytásek
R
R
Sainerová
H
H
Mandys
V
V
Sovová
V
V
Hejnar
J
J
Sloncová
E
E
Poucková
P
P
Bubeník
J
J
Nováak
J
J
D000368:Aged; D000818:Animals; D002277:Carcinoma; D002455:Cell Division; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D006801:Humans; D007621:Karyotyping; D007680:Kidney Neoplasms; D008297:Male; D051379:Mice; D008819:Mice, Nude; D013997:Time Factors; D014407:Tumor Cells, Cultured / Q000166:cytology / Q000378:metabolism*