[Radiometric detection of nonvisualizable immunoprecipitates formed by hepatoma-associated hetero-organic renal antigens of 33P-phosphorylated fractions of non-histone chromatin proteins].
A study was made of the antigenic properties of nuclear nonhistone proteins (NHP) from rat tissue eluted with 0.4-0.5 M NaCl gradient on phosphocellulose at very low concentrations. 33P-labeled proteins were obtained by phosphorylation of NHP fractions in the presence of [gamma-33P] ATP. Some non-visual precipitates which appeared during the reaction of specific inhibition of precipitation in agarose gel of 33P-labeled NHP-fractions from rat solid hepatoma 27 cells or rat liver after single hepatocarcinogen diethylnitrosamine injection were detected by means of a calculable radiometric method, proposed by the authors. These precipitates appear only if the rabbit immune sera against NHP-DNA complexes of intact rat kidneys exhausted with liver NHP-antigens was used, never appearing in the case of kidney NHP-antigen exhaustion. Therefore the NHP-fractions prepared from "carcinogenic" liver and rat solid hepatoma cells which possess proper phosphoprotein kinase activity and form specific immune precipitates must be identified as hetero-organic antigens of kidney origin.
Kushner
V P
VP
Grandilevskaia
A B
AB
Ivanova
V A
VA
Fel'
V Ia
VIa
D000255:Adenosine Triphosphate; D000818:Animals; D015478:Antigens, Heterophile / Q000032:analysis*; D000951:Antigens, Neoplasm / Q000032:analysis*; D002852:Chromatography, Ion Exchange / Q000379:methods; D002868:Chromosomal Proteins, Non-Histone / Q000276:immunology*; D007668:Kidney / Q000276:immunology*; D008099:Liver / Q000276:immunology; D008114:Liver Neoplasms, Experimental / Q000276:immunology*; D008297:Male; D010761:Phosphorus Radioisotopes; D010766:Phosphorylation; D011233:Precipitin Tests; D011874:Radiometry / Q000379:methods; D051381:Rats
D015478:Antigens, Heterophile; D000951:Antigens, Neoplasm; D002868:Chromosomal Proteins, Non-Histone; D010761:Phosphorus Radioisotopes; D000255:Adenosine Triphosphate
Radiometricheskoe vyiavlenie nevizualiziruemykh immunopretsipitatov, obrazuemykh assotsiirovannymi s gepatomami geteroorgannymi antigenami pochechnoĭ prirody 33P-fosforilirovannykh fraktsiĭ negistonovykh belkov khromatina.