title: An abundant 56 kD protein with low affinity androgen binding: another member of the steroid/thyroid receptor family?
abstract: We have found in genital skin fibroblasts an abundant 56 kD protein which appears related to the androgen receptor. The protein is detected in the soluble fraction by two-dimensional polyacrylamide gel electrophoresis as two spots with isoelectric points of 6.7 and 6.5 respectively. Photoaffinity labelling of GSF with 8 nM or 50 nM [3H]-methyltrienolone selectively labels the two protein spots but with an apparently lower affinity than is known for the androgen receptor. The two protein spots stem from one protein as judged from peptide patterns of partial proteolytic digests and the equal labelling of both spots with methyltrienolone. Cells from subjects with mutant androgen receptors generally lack the 56 kD protein and labelling with methyltrienolone fails, but the protein is not the androgen receptor itself. We propose the hypothesis that the 56 kD protein is synthesized from the same gene as the androgen receptor and that androgen may not be its natural ligand.
authors.affiliation: Department of Biochemistry, University of Manitoba, Winnipeg, Canada.
authors.forename: K
authors.initials: K
authors.lastname: Wrogemann
authors.forename: F
authors.initials: F
authors.lastname: Pereira
authors.forename: D
authors.initials: D
authors.lastname: Belsham
authors.forename: M
authors.initials: M
authors.lastname: Kaufman
authors.forename: L
authors.initials: L
authors.lastname: Pinsky
authors.forename: E
authors.initials: E
authors.lastname: Rosenmann
mesh_terms: D000728:Androgens / Q000378:metabolism*; D004963:Estrenes / Q000378:metabolism; D005347:Fibroblasts / Q000032:analysis; D005835:Genitalia / Q000166:cytology*; D006801:Humans; D007526:Isoelectric Point; D015741:Metribolone; D008970:Molecular Weight; D009154:Mutation; D010782:Photolysis; D011506:Proteins / Q000302:isolation & purification* / Q000378:metabolism; D011944:Receptors, Androgen / Q000235:genetics / Q000378:metabolism*
chemical_list: D000728:Androgens; D004963:Estrenes; D011506:Proteins; D011944:Receptors, Androgen; D015741:Metribolone