Methylglucamine orotate does not affect dopamine-stimulated fucosylation of rat hippocampal proteins.
The present paper is concerned with the question whether dopamine-stimulated fucosylation of glycoproteins of the rat hippocampus in vitro is augmented by treating animals with methylglucamine orotate (MGO). Dopamine (DA) in concentrations between 0.1-0.5 mM resulted in a dose-dependent increase in L-fucose incorporation of up to about 130%. Neither the acute intraperitoneal application of 225 mg MGO/kg body mass nor the daily treatment of rats with 225 mg MGO/kg body mass over a 10-day period changed this dose-dependent relationship significantly. Even the in vitro substitution of nucleotides (1 mM) such as uridine 5'-monophosphate, cytidine 5'-monophosphate, guanosine 5'-monophosphate, and adenosine 5'-monophosphate did not alter dopamine-stimulated fucosylation of rat hippocampal proteins. These findings suggest that DA-stimulated fucosylation of brain proteins is not limited by the supply of nucleotides.
Pchalek
V
V
Institut für Neurobiologie und Hirnforschung, Akademie der Wissenschaften, DDR.
Lössner
B
B
Matthies
H
H
D000818:Animals; D004298:Dopamine / Q000494:pharmacology*; D005643:Fucose / Q000378:metabolism*; D006031:Glycosylation; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism*; D007700:Kinetics; D008536:Meglumine / Q000031:analogs & derivatives* / Q000494:pharmacology; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009963:Orotic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D013012:Sorbitol / Q000031:analogs & derivatives*
D009419:Nerve Tissue Proteins; D005643:Fucose; D013012:Sorbitol; C022100:methylglucamine orotate; D009963:Orotic Acid; D008536:Meglumine; D004298:Dopamine