In vitro synthesis of fluorescein monoglucuronide.
A simple, economical method for the in vitro synthesis of milligram amounts of fluorescein monoglucuronide is presented. The compound can be synthesized in 1 day, and 2 chromatographic steps result in complete purification. The synthetic fluorescein monoglucuronide was found to be identical to biosynthetic fluorescein monoglucuronide by spectrophotometric and fluorometric criteria.
Guenthner
T M
TM
Department of Pharmacology, University of Illinois College of Medicine, Chicago.
Blair
N P
NP
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005452:Fluoresceins / Q000302:isolation & purification / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D008722:Methods; D008862:Microsomes, Liver / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013053:Spectrophotometry
D005452:Fluoresceins; C041357:fluorescein glucuronide