Inhibition of phytohemagglutinin (PHA) induced human T-cell colonies by methylprednisolone.
The mechanisms underlying the inhibition of T-cell proliferation by methylprednisolone (MPS) have been investigated using a T-cell colony assay. Cell fractionation experiments have demonstrated that MPS exerted its effects at the level of both T-cells and monocytes. Thus, monocytes treated with MPS released a soluble factor that had suppressor activity on T-cell proliferation. Moreover, MPS directly blocked the proliferative capacity of T-cells, as demonstrated by the finding that MPS-treated T-cells formed reduced numbers of colonies even under optimal culture conditions and in the presence of exogenous IL-2.
Ghio
R
R
Cattedra di Terapia Medica, Universitá di Genova, Italy.
Pistoia
V
V
Romagnoli
M
M
D'Elia
P
P
Boccaccio
G P
GP
D000938:Antigen-Presenting Cells / Q000187:drug effects; D002469:Cell Separation; D002478:Cells, Cultured; D003470:Culture Media; D006801:Humans; D008775:Methylprednisolone / Q000494:pharmacology*; D010835:Phytohemagglutinins / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000187:drug effects*
D003470:Culture Media; D010835:Phytohemagglutinins; D008775:Methylprednisolone