Heparin inhibits the inositol 1,4,5-trisphosphate-induced Ca2+ release from rat liver microsomes.
Inositol 1,4,5-trisphosphate (Ins (1,4,5)P3)-stimulated Ca2+ release is inhibited by low concentrations of heparin (IC50 = 4.5 micrograms/ml). GTP-stimulated Ca2+ release is unaffected at a heparin concentration of 16 micrograms/ml. Addition of heparin after Ins (1,4,5)P3 causes the rapid re-uptake of Ins (1,4,5)P3-releasable Ca2+.
Cullen
P J
PJ
School of Biological Sciences, University of East Anglia, Norwich, England.
Comerford
J G
JG
Dawson
A P
AP
D000818:Animals; D002118:Calcium / Q000493:pharmacokinetics*; D006160:Guanosine Triphosphate / Q000494:pharmacology; D006493:Heparin / Q000494:pharmacology*; D066298:In Vitro Techniques; D015544:Inositol 1,4,5-Trisphosphate; D007295:Inositol Phosphates / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism*; D051381:Rats; D013403:Sugar Phosphates / Q000494:pharmacology*
D007295:Inositol Phosphates; D013403:Sugar Phosphates; D015544:Inositol 1,4,5-Trisphosphate; D006160:Guanosine Triphosphate; D006493:Heparin; D002118:Calcium