The glycosaminoglycans of the macrophage-like cell line PeMa in suspension and monolayer culture.
The macrophage-like cell line PeMa was cultured both in suspension and in monolayer for biochemical studies. It is concluded that adherent PeMa when compared with suspended cells --contain three times more GAG in medium, cell coat, and cells: --contain higher amounts of HS and CS in the cell coat; --release a minor proportion of sulfated GAG (HS) into the medium; --contain in their medium longer chains of HS and CS/DS; --contain in cell coat and cells higher amounts of a family of HS chains with high to low molecular weights. In suspension and monolayer cultures, the cell coat GAG were scarcely labelled; they are likely to be produced at another time and to turn over slowly. It is suggested that this study will be helpful to understand the behaviour of macrophages and other cells.
Kittlick
P D
PD
Friedrich Schiller University Jena, Institute of Pathology, DDR.
Engelmann
D
D
D000818:Animals; D002460:Cell Line; D002850:Chromatography, Gel / Q000379:methods; D006025:Glycosaminoglycans / Q000032:analysis* / Q000096:biosynthesis; D008264:Macrophages / Q000032:analysis*; D008970:Molecular Weight; D013431:Sulfates / Q000378:metabolism; D013462:Sulfur Radioisotopes
D006025:Glycosaminoglycans; D013431:Sulfates; D013462:Sulfur Radioisotopes