Oxygen consumption of single muscle fibres of Rana temporaria and Xenopus laevis at 20 degrees C.
1. Oxygen consumption of contracting single muscle fibres of Rana temporaria and Xenopus laevis was investigated at 20 degrees C. 2. Single fibres of the tibialis anterior muscle of Rana and the iliofibularis muscle of Xenopus were mounted in a chamber containing Ringer solution. The solution was stirred and its partial pressure of oxygen (PO2) was continuously measured polarographically. 3. Steady-state rates of oxygen consumption (VO2) of single fibres were determined as a function of twitch frequency (0.2-12 stimuli s-1, depending on the type of fibre). VO2 increased with twitch frequency until a plateau value (VO2,max) was reached. VO2,max of different fibres ranged from 0.042 to 0.169 nmol O2 s-1 mg-1 dry weight in Rana and from 0.045 to 0.412 nmol O2 s-1 mg-1 dry weight in Xenopus. Under VO2,max conditions oxygen availability was not the limiting factor. 4. VO2 after injection of the uncoupler carbonyl cyanide m-chlorophenylhydrazone (CCCP) into the chamber correlated with VO2,max, suggesting that VO2,max is determined by mitochondrial density. This suggestion was confirmed by the observation that a close relationship exists between VO2,max and succinate dehydrogenase activity in three different fibre types of Xenopus. 5. At VO2,max a considerable amount of oxygen was taken up after the twitch train by most fibres, indicating that the oxidative ATP synthesis cannot match ATP hydrolysis. Xenopus muscle fibres with high oxidative capacity did not show this phenomenon. 6. The results are discussed in relation to the occurrence of anoxic cores in muscle fibres and the maximum steady-state contractile activity attainable by the fibres.
Elzinga
G
G
Laboratory for Physiology, Free University, Amsterdam, The Netherlands.
van der Laarse
W J
WJ
D000818:Animals; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology; D005260:Female; D009119:Muscle Contraction; D009132:Muscles / Q000187:drug effects / Q000378:metabolism*; D010085:Oxidative Phosphorylation / Q000187:drug effects; D010101:Oxygen Consumption / Q000187:drug effects; D011896:Rana temporaria; D014982:Xenopus laevis
D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone