Dimethyl sulfoxide inhibits proliferation but not hypertrophy and functional dedifferentiation of bovine parathyroid cells in culture.
Primary cultures of bovine parathyroid cells were used to study the relationship between parathyroid hormone (PTH) release, regulation of the cytoplasmic Ca2+ concentration (Ca2+i) and cell growth. Parathyroid cells were seeded into fibronectin-coated culture wells in the absence (control) or presence of 2% (v/v) dimethyl sulfoxide (DMSO). Both groups of cells attached and flattened out, and during 4 days of culture the cell diameters and protein content increased. However, whereas the number of cells in the control group increased by 60% and their 3H-thymidine incorporation tenfold, the DMSO cultured cells exhibited no signs of cell proliferation. Despite this difference in cell replication rate both groups of cells developed similar functional dedifferentiation. There was consequently a decreased Ca2+ suppressibility of PTH release, and Ca2+i did not follow the ambient Ca2+ concentration in a usual manner. It is concluded that during culture bovine parathyroid cells develop morphological and functional abnormalities similar those in parathyroid cells from patients with hyperparathyroidism. Moreover, this functional dedifferentiation could be dissociated from increased cell proliferation but may be related to cell hypertrophy.
Nygren
P
P
Department of Medical Cell Biology, University of Uppsala, Sweden.
Larsson
R
R
Lindh
E
E
Rastad
J
J
Akerström
G
G
Gylfe
E
E
D000818:Animals; D002118:Calcium / Q000378:metabolism; D002417:Cattle; D002452:Cell Count; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004247:DNA / Q000378:metabolism; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D005434:Flow Cytometry; D006984:Hypertrophy / Q000473:pathology; D010280:Parathyroid Glands / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D010281:Parathyroid Hormone / Q000378:metabolism
D010281:Parathyroid Hormone; D004247:DNA; D002118:Calcium; D004121:Dimethyl Sulfoxide