Multigene families of Cellulomonas flavigena encoding endo-beta-1,4-glucanases (CM-cellulases).
Multiple genes coding for endo-beta-1,4-glucanases (CM-cellulases) have been isolated from a newly discovered highly cellulolytic strain of Cellulomonas flavigena. Clones of C. flavigena DNA were isolated in Escherichia coli and screened for gene expression on CM-cellulose plates staining with congo red. Six clones produced CM-cellulase activity as detected in liquid assays, and on activity gels. They fell into three groups within which the sequences cross-hybridised. There were small differences in the pH and temperature optima of the enzymes encoded by representatives of the three groups of clones.
Akhtar
M W
MW
Department of Genetics, Trinity College, Dublin, Ireland.
Duffy
M
M
Dowds
B C
BC
Sheehan
M C
MC
McConnell
D J
DJ
D001419:Bacteria / Q000235:genetics*; D002480:Cellulase / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D005786:Gene Expression Regulation; D006863:Hydrogen-Ion Concentration; D005810:Multigene Family; D010957:Plasmids; D012689:Sequence Homology, Nucleic Acid; D013696:Temperature
D004247:DNA; D002480:Cellulase