title: Purification and partial amino acid sequencing of alkaline phosphatase from rachitic rat epiphyseal cartilage.
abstract: 1. Alkaline phosphatase of rachitic epiphyseal cartilage was purified to apparent homogeneity by sequential application of monoclonal affinity, DEAE-cellulose, and Sepharose CL-6B chromatography. Sodium dodecyl sulfate polyacrylamide gel electrophoresis of the enzyme showed the presence of a dominant band corresponding to a molecular weight of 80,000. 2. The N-terminal amino acid sequence was determined as follows: Phe-Val-Pro-Glu-Lys5-Glu-Lys-Asp-Pro-Ser10-Tyr-Trp-Arg-Gln-+ ++Gln15-Ala-Gln-Glu- Thr-Leu20-Lys-Asn-Ala-Leu-Lys25-Leu-Gln-Lys-?-Asn-Val-Asn-?- Ala-Lys35-?-Ile-?- Met-Phe40-Leu-(Gly?)-Asp-(Ala/Gly?)-Met45-?-Val-?- (Val/Gly?).
authors.affiliation: Department of Pathology and Oncology, University of Kansas Medical Center, Kansas City 66103.
authors.forename: H H
authors.initials: HH
authors.lastname: Hsu
authors.forename: J
authors.initials: J
authors.lastname: Rouse
authors.forename: J
authors.initials: J
authors.lastname: Hamilton
authors.forename: H C
authors.initials: HC
authors.lastname: Anderson
mesh_terms: D000469:Alkaline Phosphatase / Q000276:immunology / Q000302:isolation & purification*; D000595:Amino Acid Sequence; D000596:Amino Acids / Q000302:isolation & purification; D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification; D000915:Antibody Affinity; D002356:Cartilage / Q000201:enzymology*; D002848:Chromatography, DEAE-Cellulose; D004591:Electrophoresis, Polyacrylamide Gel; D008969:Molecular Sequence Data; D051381:Rats; D012279:Rickets / Q000201:enzymology*
chemical_list: D000596:Amino Acids; D000911:Antibodies, Monoclonal; D000469:Alkaline Phosphatase