Theoretical analysis of a site-specific chemiluminescence reaction and its application to quantitation of lipid hydroperoxides.
A system was designed for chemiluminescent measurement of lipid hydroperoxides by their site-specific reaction in sodium dodecylsulfate micelles. Ferrous ion-induced decomposition of lipid hydroperoxides in the sodium dodecylsulfate micelles resulted in strong chemiluminescence of the Cypridina luciferin analog, 2-methyl-6-phenyl-3,7-dihydroimidazo[1,2-alpha]pyrazin-3-one (CLA). After addition of ferrous sulfate to the micelles containing lipid hydroperoxide and luciferin, the chemiluminescence intensity reached a maximum rapidly and then decreased. The sequence of this reaction was elucidated by theoretical analysis, which demonstrated that the maximum chemiluminescence intensity is proportional to the initial concentration of hydroperoxide. Good linear relationships were observed between the maximum counts of chemiluminescence and the amounts of hydroperoxides of linoleic acid, phosphatidylcholine, choresterol (5 alpha), cumene and tert-butyl and hydrogen peroxide. This chemiluminescence method was simple and sensitive enough to detect picomole levels of linoleic acid and phosphatidylcholine hydroperoxides.
Fukuzawa
K
K
Faculty of Pharmaceutical Sciences, University of Tokushima, Japan.
Yoshimura
T
T
Fujii
T
T
Takauchi
K
K
Miki
M
M
Tamai
H
H
Mino
M
M
D001665:Binding Sites; D005296:Ferrous Compounds / Q000378:metabolism; D008054:Lipid Peroxides / Q000032:analysis*; D008163:Luminescent Measurements; D008823:Micelles; D010084:Oxidation-Reduction
D005296:Ferrous Compounds; D008054:Lipid Peroxides; D008823:Micelles