C5a stimulates secretion of tumor necrosis factor from human mononuclear cells in vitro. Comparison with secretion of interleukin 1 beta and interleukin 1 alpha.
We have demonstrated that purified C5a is a potent stimulus to human PBMC secretion of TNF-alpha, IL-1 beta, and IL-1 alpha, which proceeds in a dose-dependent fashion. At a given concentration of C5a, TNF-alpha and IL-1 beta secretion did not differ significantly; both were secreted in significantly greater quantity than IL-1 alpha. Clinical conditions such as Gram-positive and Gram-negative bacterial infections, trauma, and immune complex diseases activate complement. Through the mediation of TNF and IL-1 secreted in response to C5a, these diverse disorders can share common features of fever, coagulopathy, acute phase protein production, and disordered metabolism.
Okusawa
S
S
Department of Medicine, Tufts University, Boston, Massachusetts 02111.
Yancey
K B
KB
van der Meer
J W
JW
Endres
S
S
Lonnemann
G
G
Hefter
K
K
Frank
M M
MM
Burke
J F
JF
Dinarello
C A
CA
Gelfand
J A
JA
D003182:Complement C5 / Q000502:physiology*; D015936:Complement C5a; D003601:Cytotoxicity Tests, Immunologic; D006801:Humans; D066298:In Vitro Techniques; D007375:Interleukin-1 / Q000502:physiology*; D007963:Leukocytes, Mononuclear / Q000502:physiology*; D011112:Polymyxin B / Q000494:pharmacology; D011863:Radioimmunoassay; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D003182:Complement C5; D007375:Interleukin-1; D014409:Tumor Necrosis Factor-alpha; D015936:Complement C5a; D011112:Polymyxin B