Basophils exhibit rearrangement of the bcr gene in Philadelphia chromosome-positive chronic myeloid leukemia.
Basophils were isolated from the peripheral blood of two patients with Philadelphia positive-chronic myeloid leukemia using monoclonal antibody Bsp-1 and fluorescence activated cell sorting. DNA blot analyses demonstrated rearrangement of the breakpoint cluster region gene in the isolated basophils, which suggests their leukemic origin. Isolated T cells from these patients that were cultured for 14 days in the presence of interleukin-2 lacked rearrangement of the breakpoint cluster region gene and are therefore unlikely to be derived from the chronic myeloid leukemia clone.
Bosma
T J
TJ
Cancer Society of New Zealand, Cytogenetics Unit, Christchurch Hospital.
Kennedy
M A
MA
Bodger
M P
MP
Hollings
P E
PE
Fitzgerald
P H
PH
D001491:Basophils / Q000032:analysis / Q000473:pathology*; D006801:Humans; D007951:Leukemia, Myeloid / Q000235:genetics* / Q000473:pathology; D009693:Nucleic Acid Hybridization; D009857:Oncogenes; D010677:Philadelphia Chromosome; D011995:Recombination, Genetic; D013601:T-Lymphocytes / Q000032:analysis / Q000473:pathology