title: Insulin-like growth factor-I stimulates oxytocin and progesterone production by bovine granulosa cells in culture.
abstract: The effect of insulin-like growth factor-I (IGF-I), epidermal growth factor (EGF), fibroblast growth factor (FGF) and nerve growth factor (NGF) on production of oxytocin and progesterone by cultured bovine granulosa and luteal cells was studied. Secretion of oxytocin was stimulated, in a dose-dependent manner, by IGF-I at 48 and 120 h of culture to levels much higher than those after stimulation with LH, FSH, EGF, FGF or NGF. A similar effect of IGF-I was observed for progesterone but, in contrast to oxytocin, secretion of progesterone was not increased by EGF, NGF or FGF. During primary culture, for 4 h, of dispersed bovine luteal cells obtained from corpora lutea between days 4 and 10 of the oestrous cycle, all the growth factors tested failed to stimulate secretion of oxytocin or progesterone. The data suggest the relevance of growth factors (especially IGF-I) for ovarian physiology and their possible importance for differentiation of follicles and luteinization.
authors.affiliation: Lehrstuhl für Physiologie der Fortpflanzung und Laktation, Technische Universität Müchen, Freising-Weihenstephan, Federal Republic of Germany.
authors.forename: D
authors.initials: D
authors.lastname: Schams
authors.forename: R
authors.initials: R
authors.lastname: Koll
authors.forename: C H
authors.initials: CH
authors.lastname: Li
mesh_terms: D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D004815:Epidermal Growth Factor / Q000502:physiology; D005260:Female; D005346:Fibroblast Growth Factors / Q000502:physiology; D006107:Granulosa Cells / Q000378:metabolism*; D007334:Insulin-Like Growth Factor I / Q000502:physiology*; D008184:Luteal Cells / Q000378:metabolism; D009414:Nerve Growth Factors / Q000502:physiology; D010121:Oxytocin / Q000378:metabolism*; D011374:Progesterone / Q000378:metabolism*; D013002:Somatomedins / Q000502:physiology*
chemical_list: D009414:Nerve Growth Factors; D013002:Somatomedins; D011374:Progesterone; D010121:Oxytocin; D005346:Fibroblast Growth Factors; D004815:Epidermal Growth Factor; D007334:Insulin-Like Growth Factor I