Gene for selenium-dependent glutathione peroxidase maps to human chromosomes 3, 21 and X.
A human glutathione peroxidase cDNA has been used as a probe to hybridize to DNAs isolated from human - rodent somatic cell hybrids that have segregated human chromosomes. A 609 bp probe which contains the entire coding region hybridizes to human chromosomes 3, 21 and Xp. Fragments of the cDNA coding sequence and of the 3' untranslated region were also used as probes. These fragments hybridized to each of the three chromosomes with the same efficiency, suggesting similarity between the loci, whereas an intronic probe detected only the gene on chromosome 3. The general organization of each gene was determined from the hybridization data. The data suggest that the locus on chromosome 3 is a functional gene containing a single intron and a pattern of restriction sites identical to those found in the cDNA coding sequence. The data also suggest that the sequences on chromosomes X and 21 have equal conservation of the 3' untranslated and coding sequences but do not contain introns, providing evidence that the latter two sequences are processed pseudogenes. A simple two allele polymorphism in PvuII digests was detected at the locus on chromosome 21.
McBride
O W
OW
Laboratory of Biochemistry, National Cancer Institute, Bethesda, MD 20892.
Mitchell
A
A
Lee
B J
BJ
Mullenbach
G
G
Hatfield
D
D
D000818:Animals; D015139:Blotting, Southern; D002874:Chromosome Mapping; D002891:Chromosomes, Human, Pair 21; D002893:Chromosomes, Human, Pair 3; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D005796:Genes; D005979:Glutathione Peroxidase / Q000235:genetics*; D006801:Humans; D006822:Hybrid Cells / Q000201:enzymology; D051379:Mice; D009693:Nucleic Acid Hybridization; D011506:Proteins / Q000235:genetics*; D011544:Pseudogenes; D015183:Restriction Mapping; D012643:Selenium / Q000378:metabolism; D051140:Selenoproteins; D014960:X Chromosome
D011506:Proteins; D051140:Selenoproteins; D004247:DNA; D005979:Glutathione Peroxidase; D012643:Selenium