Method for simultaneous determination of creatine phosphate and adenine nucleotides in the intestinal smooth muscle of guinea-pig taenia caeci using high-performance liquid chromatography.
A method for simultaneous determination of adenosine 5'-triphosphate (ATP), adenosine 5'-diphosphate (ADP) and creatine phosphate (PCr) by high-performance liquid chromatography is described. This method was applied to the isolated intestinal smooth muscle tissue of guinea pig taenia caeci weighing approximately 30 mg. It was found that one g of the muscle tissue contained 3.55 mumol PCr, 2.40 mumol ATP and 0.477 mumol ADP.
Endo
I
I
Terumo R & D Division, Tokyo, Japan.
Utsugi
M
M
Suzuki
T
T
Karaki
H
H
D000244:Adenosine Diphosphate / Q000032:analysis*; D000255:Adenosine Triphosphate / Q000032:analysis*; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003106:Colon / Q000032:analysis; D006168:Guinea Pigs; D066298:In Vitro Techniques; D007422:Intestines / Q000032:analysis; D008297:Male; D009130:Muscle, Smooth / Q000032:analysis*; D010725:Phosphocreatine / Q000032:analysis*
D010725:Phosphocreatine; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate