EGF receptor expression in the developing tooth is altered by exogenous retinoic acid and EGF.
Retinoic acid (RA) is a teratogen producing a variety of malformations including clefts of the secondary palate and malformations of the teeth. Recent studies in our laboratory investigating the effects of RA and EGF on the medial epithelium of the palatal shelf have also provided some information on the effects of these agents on the development of the tooth. In the control, toothbud epithelial cells expressed the EGF receptor, bound EGF, and proliferated. The majority of bud stage teeth exposed to RA either in vivo or in organ culture did not detectably bind EGF, express the EGF receptor, or proliferate in the epithelial or mesenchymal components. Toothbuds exposed to exogenous EGF in organ culture also did not bind EGF, the receptor, or proliferate. EGF has been reported to inhibit morphogenesis of toothbuds in culture and when EGF was given to neonates the size of the tooth was reduced. Regulation of EGF receptor expression appears to be important in the development of the toothbud and exposure to RA or EGF disrupts this process and could contribute to the decreased tooth size, agenesis, and malformations observed in the teeth. While limited in scope, these experiments present previously unreported effects of RA and EGF on the toothbud epithelium. These observations should be of interest to those studying tooth development, and warrant further and more detailed studies.
Abbott
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BD
Laboratory of Reproductive and Developmental Toxicology, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709.
Pratt
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RM
D000818:Animals; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D066246:ErbB Receptors / Q000096:biosynthesis*; D005260:Female; D051379:Mice; D009805:Odontogenesis / Q000187:drug effects*; D011247:Pregnancy; D014212:Tretinoin / Q000494:pharmacology*
D014212:Tretinoin; D004815:Epidermal Growth Factor; D066246:ErbB Receptors