title: Intracellular pH in platelets from spontaneously hypertensive rats and Wistar-Kyoto rats.
abstract: Intracellular pH in platelets from spontaneously hypertensive rats (SHR) and age-matched Wistar-Kyoto rats (WKY) was measured using a pH-sensitive fluorescent dye, 2',7'-bis(carboxyethyl)carboxyfluorescein tetraacetoxy methyl ester (BCECF). Intracellular pH in platelets was significantly higher in SHR than in WKY (7.12 +/- 0.04 versus 7.06 +/- 0.02, P less than 0.01; n = 10). There was a close correlation between intracellular pH and systolic blood pressure (r = 0.66, P less than 0.01). Addition to platelets of 1-(5-isoquinolinesulphonyl)-2-methylpiperazine (H-7), a specific inhibitor of protein kinase C, produced a significantly larger decrease in intracellular pH in SHR than in WKY (0.06 +/- 0.02 versus 0.03 +/- 0.00, P less than 0.01, n = 5). These results suggest that protein kinase C may play an important role in the increased intracellular pH in SHR.
authors.affiliation: First Department of Internal Medicine, Osaka City University Medical School, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Inariba
authors.forename: Y
authors.initials: Y
authors.lastname: Kanayama
authors.forename: K
authors.initials: K
authors.lastname: Takaori
authors.forename: S
authors.initials: S
authors.lastname: Itoh
authors.forename: T
authors.initials: T
authors.lastname: Inoue
authors.forename: T
authors.initials: T
authors.lastname: Takeda
mesh_terms: D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D000818:Animals; D001792:Blood Platelets / Q000502:physiology*; D006863:Hydrogen-Ion Concentration; D006973:Hypertension / Q000503:physiopathology*; D007546:Isoquinolines / Q000494:pharmacology; D010879:Piperazines / Q000494:pharmacology; D011493:Protein Kinase C / Q000502:physiology*; D051381:Rats; D011918:Rats, Inbred SHR / Q000502:physiology*; D011919:Rats, Inbred Strains / Q000502:physiology*; D011921:Rats, Inbred WKY / Q000502:physiology; D013449:Sulfonamides
chemical_list: D007546:Isoquinolines; D010879:Piperazines; D013449:Sulfonamides; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; C043204:N-(2-guanidinoethyl)-5-isoquinolinesulfonamide; D011493:Protein Kinase C