Age-dependent responses of ciliary ganglion neurons to conditioned media on cells at different stages of embryonic development.
Neurons from ciliary ganglia (CG) from 8 to 14 day-old chick embryos were cultured in presence of conditioned media (CM) by eye tissue cells (ETC) on nonneuronal cells from ciliary ganglia (NFGC). These conditioning cells were obtained from 8 and 14 day-old embryos. Two parameters, surviving neurons and neurons displaying neurites, were determined after 48 h of culture. For neuronal survival, CMs did not show an effect on CG14 neurons. In the other neuronal ages ETC-CMs maintained a similar neuronal survival, whereas NFGC-CMs were more effective on older neurons. CM14 media were more effective maintaining neuronal survival than CM8 media respectives. The number of neurons displaying neurites decreased with neuronal ages in presence of all CMs. ETC8-CM was the better promoting neurite extension in all neuronal ages tested.
Alcain
F J
FJ
Departamento de Ciencias Morfológicas, Facultad de Veterinaria, Universidad de Córdoba, Spain.
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002642:Chick Embryo; D003470:Culture Media / Q000494:pharmacology; D003712:Dendrites / Q000187:drug effects / Q000502:physiology; D004622:Embryo, Mammalian; D004625:Embryo, Nonmammalian; D005123:Eye / Q000166:cytology / Q000378:metabolism*; D005726:Ganglia, Parasympathetic / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D009414:Nerve Growth Factors / Q000378:metabolism / Q000494:pharmacology*
D003470:Culture Media; D009414:Nerve Growth Factors