Cryoglobulinemia: analysis of isotype, idiotype and antibody activity by composite gel electrophoresis and immunoblotting.
Improved methods for high resolution composite gel electrophoresis under dissociating conditions and electrophoretic transfer of immunoglobulins (Mr 150,000-1,800,000) to nitrocellulose have been developed. Using these techniques and highly specific antisera to detect either light or heavy chains on nitrocellulose transfers, the immunoglobulin and clonal composition of washed cryoglobulins (3 micrograms each) could be determined within two days. The results were confirmed by isoelectric focusing and immunoblotting of selected samples. In addition, the method was used for detection of an idiotypic determinant and rheumatoid factor activity within components of the cryoglobulins.
Chu
J L
JL
Hospital for Special Surgery, Cornell University Medical Center, New York, NY 10021.
Gharavi
A E
AE
Elkon
K B
KB
D000906:Antibodies / Q000032:analysis*; D003449:Cryoglobulinemia / Q000276:immunology*; D003450:Cryoglobulins / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D015151:Immunoblotting; D007130:Immunoglobulin Idiotypes / Q000032:analysis*; D007132:Immunoglobulin Isotypes / Q000032:analysis*; D007525:Isoelectric Focusing
D000906:Antibodies; D003450:Cryoglobulins; D007130:Immunoglobulin Idiotypes; D007132:Immunoglobulin Isotypes