Accessory cell function of human melanoma cells in mitogen-induced T cell proliferation.
Six out of eight human melanoma cell lines were found to be able to function as accessory cells in PHA-induced proliferation of autologous and allogeneic T cells. The accessory cell function of the melanoma cell lines appears to be similar to that of monocytes, requires the presence of viable cells, and does not correlate with the cell surface binding sites for PHA and with the level of expression of HMW-MAA and of HLA Class I antigens. HLA Class II antigens do not appear to play a major role in these phenomena, since there is no relationship between level of expression of HLA Class II antigens and accessory cell function of melanoma cells. Furthermore, addition of anti-HLA Class II monoclonal antibodies does not affect proliferation of T cells stimulated with PHA in the presence of melanoma cells with accessory cell function. Although melanoma cells exert accessory cell function, functional and immunological assays did not detect IL-1 in the spent medium of the melanoma cell lines. Furthermore, Northern blotting analysis with IL-1 alpha and IL-1 beta probes did not detect IL-1-specific mRNA in melanoma cell lines. These results suggest that PHA-induced proliferation of T cells in the presence of melanoma cells can bypass the requirement for IL-1 or utilizes factors other than IL-1.
Cordiali-Fei
P
P
Laboratory of Immunology, S. Gallicano Institute of Dermatology, Rome, Italy.
Mottolese
M
M
Tecce
R
R
Natali
P
P
Ferrone
S
S
D000938:Antigen-Presenting Cells / Q000276:immunology*; D015152:Blotting, Northern; D004306:Dose-Response Relationship, Immunologic; D006681:HLA-D Antigens / Q000276:immunology; D006801:Humans; D066298:In Vitro Techniques; D007375:Interleukin-1 / Q000032:analysis / Q000235:genetics; D008213:Lymphocyte Activation; D008545:Melanoma / Q000276:immunology*; D009000:Monocytes / Q000502:physiology; D010835:Phytohemagglutinins / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics; D015375:Receptors, Interleukin-2 / Q000032:analysis; D013601:T-Lymphocytes / Q000502:physiology*; D014407:Tumor Cells, Cultured
D006681:HLA-D Antigens; D007375:Interleukin-1; D010835:Phytohemagglutinins; D012333:RNA, Messenger; D015375:Receptors, Interleukin-2