Oxygen concentration regulates EGF-induced proliferation and EGF-receptor down regulation.
Reducing oxygen from 20% to 2.5% increases EGF-induced DNA synthesis and cell proliferation in cultures of human diploid fibroblasts. Reducing oxygen also changes the pattern of EGF binding to the cell surface. The loss of surface binding that follows EGF attachment to cells in 20% oxygen does not occur in 2.5% oxygen.
Wing
D A
DA
Department of Pediatrics, Tulane University School of Medicine, New Orleans, LA 70112.
Talley
G D
GD
Storch
T G
TG
D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D066246:ErbB Receptors / Q000378:metabolism*; D006801:Humans; D010100:Oxygen / Q000494:pharmacology*; D013997:Time Factors
D004815:Epidermal Growth Factor; D066246:ErbB Receptors; D010100:Oxygen