[Determination of the enzymatic activity of lysine amidase].
A technique is proposed for determining lysinamidase and aminolactamase activities of lysinamidase (EC 3.5.1.n.). It is based on spectrophotometric measurement of the optical density decrease of the substrate solution at 227 nm. For cyclic lysinamide L-alpha-amino-epsilon-caprolactam epsilon 227 M = 151 M-1.cm-1, for linear lysinamide epsilon 227 M = 73 M-1.cm-1, and for lysine epsilon 227 M = 5 M-1.cm-1. The technique is simple and requires no additional reagents.
Cheskis
B I
BI
Shebeka
G B
GB
Pauliukonis
A B
AB
Kazlauskas
D A
DA
D000581:Amidohydrolases / Q000032:analysis*; D002138:Calibration; D002384:Catalysis; D013056:Spectrophotometry, Ultraviolet
D000581:Amidohydrolases; C040276:lysine amidase
Opredelenie fermentativnoĭ aktivnosti lizinamidazy.