Effects of HLA-B27 positive and negative sera on migration of polymorphonuclear leukocytes in vitro.
Sera from both HLA-B27 positive patients with previous yersinia arthritis and healthy HLA-B27 positive subjects have been reported to stimulate chemokinetic migration of polymorphonuclear leukocytes (PMNs) more than sera from subjects who are HLA-B27 negative. To study this further we tested chemotactic and chemokinetic activities of sera of 213 consecutive blood donors (27 positive for HLA-B27, 12.7%) and those of 55 children (mean age 20 months, range 6-137 months, 8 positive for HLA-B27, 14.5%). Migration distances of PMNs both in membrane filter and under agarose were longer, although not in a statistically significant way, in HLA-B27 positive adult sera than in HLA-B27 negative ones. Chemokinetic activity of sera of HLA-B27 positive children was very similar to that of HLA-B27 negative children. These results give credence to the view that PMN migration enhancing capacity of HLA-B27 positive sera described previously is acquired secondarily and is not genetically determined.
Repo
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Department of Bacteriology and Immunology, University of Helsinki, Finland.
Leirisalo-Repo
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M
Koskimies
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S
D000328:Adult; D001170:Arthritis, Infectious / Q000276:immunology*; D002464:Cell Migration Inhibition; D002634:Chemotaxis, Leukocyte; D002648:Child; D002675:Child, Preschool; D015235:HLA-B Antigens / Q000276:immunology*; D015796:HLA-B27 Antigen; D006801:Humans; D007223:Infant; D009504:Neutrophils / Q000276:immunology*
D015235:HLA-B Antigens; D015796:HLA-B27 Antigen