Lytic function of bovine lymphokine-activated killer cells from a normal and a malignant catarrhal fever virus-infected animal.
Lymphokine-supplemented long-term cultured bovine lymph node lymphocytes were characterized functionally and phenotypically. Lymphocytes from a normal and a malignant catarrhal fever (MCF) virus-infected animal were maintained without the addition of antigen or feeder cells. Lymphocyte cell lines obtained from both animals: (i) killed allogeneic fibroblasts and allogeneic and xenogeneic cultured tumor cell lines as measured in a 4-h 51Cr release assay, (ii) expressed the same T cell subset marker based on flow cytometry using monoclonal antibodies, and (iii) produced a lytic factor upon stimulation. In contrast, only cells from the MCF virus-infected animal could be maintained for more than 5 months supplemented with 2% Con A-generated lymphokine-containing supernatant. These results suggest that herpesvirus infection enhanced the proliferative capabilities of the cultured lymphocytes from the infected animal. Considering the proliferative and cytotoxic activity together with the T cell phenotype, these data indicated that effector cells are lymphokine-activated killer cells.
Cook
C G
CG
University of Wisconsin-Madison, Department of Veterinary Science 53706.
Splitter
G A
GA
D000818:Animals; D002417:Cattle; D002460:Cell Line; D003208:Concanavalin A / Q000494:pharmacology*; D003602:Cytotoxicity, Immunologic; D005260:Female; D005347:Fibroblasts / Q000648:ultrastructure; D007376:Interleukin-2 / Q000494:pharmacology*; D007694:Killer Cells, Natural / Q000276:immunology* / Q000648:ultrastructure; D008198:Lymph Nodes / Q000166:cytology; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology* / Q000648:ultrastructure; D008304:Malignant Catarrh / Q000276:immunology*; D008854:Microscopy, Electron; D010641:Phenotype; D013601:T-Lymphocytes / Q000276:immunology / Q000648:ultrastructure
D007376:Interleukin-2; D003208:Concanavalin A