Direct extracellular electrical stimulation influences density dependent aggregation of fetal rat cerebrocortical neurons in vitro.
Cells isolated from fetal (E17) rat cerebral cortex form aggregates in a density-dependent manner. Direct current electrical stimulation influences the formation of neuronal aggregates by promoting neuronal survival at a calculated current density of 15 nA/cm2 (with an average calculated field strength of 1 microV/cm), and inhibiting neuronal survival at calculated current densities of both 1 nA/cm2 and 150 nA/cm2. Electrical stimulation had no observable effect on neuronal aggregation independent of its effect on cell survival.
Kaplan
F S
FS
McKay Laboratory of Orthopaedic Surgery Research, University of Pennsylvania, School of Medicine, Philadelphia 19104.
Maguire
T G
TG
Lee
V M
VM
Selzer
M E
ME
Spindler
K
K
Black
J
J
Brighton
C T
CT
D000818:Animals; D002449:Cell Aggregation; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology* / Q000196:embryology; D004558:Electric Stimulation / Q000295:instrumentation / Q000379:methods; D005333:Fetus; D009474:Neurons / Q000166:cytology*; D051381:Rats; D011919:Rats, Inbred Strains