title: Mutations in human lymphocytes studied by an HLA selection system.
abstract: Human lymphocytes mutated at the HLA-A2 or HLA-A3 alleles were enumerated and studied by primary selection using antibody and complement, followed by limiting dilution cloning and secondary selection using immunofluorescence or antibody and complement. The geometric mean frequency of in vivo mutant lymphocytes was 3.08 X 10(-5) for the HLA-A2 allele and 4.68 X 10(-6) for the HLA-A3 allele. Mutagenesis by X-radiation or mitomycin produced a dose-related increase in mutant frequency. HLA-B phenotyping and Southern Analysis of the HLA-A gene suggested that mutation was frequently due to gene deletion, which was often substantial.
authors.affiliation: Department of Haematology, Flinders Medical Centre, Bedford Park, SA, Australia.
authors.forename: M
authors.initials: M
authors.lastname: Janatipour
authors.forename: K J
authors.initials: KJ
authors.lastname: Trainor
authors.forename: R
authors.initials: R
authors.lastname: Kutlaca
authors.forename: G
authors.initials: G
authors.lastname: Bennett
authors.forename: J
authors.initials: J
authors.lastname: Hay
authors.forename: D R
authors.initials: DR
authors.lastname: Turner
authors.forename: A A
authors.initials: AA
authors.lastname: Morley
mesh_terms: D000483:Alleles; D002478:Cells, Cultured; D002872:Chromosome Deletion; D006680:HLA Antigens / Q000235:genetics*; D015789:HLA-A2 Antigen; D015791:HLA-A3 Antigen; D006579:Heterozygote; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology* / Q000528:radiation effects; D008937:Mitomycins / Q000494:pharmacology; D010641:Phenotype; D012641:Selection, Genetic
chemical_list: D006680:HLA Antigens; D015789:HLA-A2 Antigen; D015791:HLA-A3 Antigen; D008937:Mitomycins