Selective cytotoxicity of an oxygen-radical-generating enzyme conjugated to a monoclonal antibody.
The monoclonal antibody 8A, which recognizes a human plasma cell-associated antigen, was covalently linked to xanthine oxidase in a conjugate maintaining both immunological and enzymatic properties. A significant degree of target cell lysis was obtained at an enzyme concentration that was ineffective on non-target cells and on myeloid staminal cells (CFU-GM). The cytotoxic activity was abolished by an excess of antibody, by allopurinol and by superoxide dismutase and catalase. A possible use of the conjugate for bone marrow purging in multiple myeloma patients is suggested.
Battelli
M G
MG
Dipartimento di Patologia Sperimentale, Istituto di Ematologia Lorenzo e Ariosto Seragnoli, Università di Bologna, Italy.
Abbondanza
A
A
Tazzari
P L
PL
Dinota
A
A
Rizzi
S
S
Grassi
G
G
Gobbi
M
M
Stirpe
F
F
D000911:Antibodies, Monoclonal / Q000276:immunology; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D005609:Free Radicals; D006412:Hematopoietic Stem Cells / Q000187:drug effects; D006801:Humans; D000922:Immunotoxins / Q000494:pharmacology*; D010100:Oxygen / Q000378:metabolism; D014407:Tumor Cells, Cultured / Q000187:drug effects; D014969:Xanthine Oxidase / Q000494:pharmacology*
D000911:Antibodies, Monoclonal; D005609:Free Radicals; D000922:Immunotoxins; D014969:Xanthine Oxidase; D010100:Oxygen