Ligand activation of overexpressed epidermal growth factor receptors transforms NIH 3T3 mouse fibroblasts.
The cell surface receptor for the mitogenic peptide epidermal growth factor (EGF) is involved in control of normal cell growth and may play a role in the genesis of human neoplasia such as squamous carcinoma and glioblastoma. Soft-agar growth and focus-formation experiments with NIH 3T3 mouse fibroblasts transfected with an expression plasmid demonstrated the ligand-dependent transforming potential of the human EGF receptor without structural alterations. Activation of overexpressed normal receptor alone appears to be sufficient for transformation of NIH 3T3 cells in vitro.
Riedel
H
H
Department of Developmental Biology, Genentech, South San Francisco, CA 94080.
Massoglia
S
S
Schlessinger
J
J
Ullrich
A
A
D000818:Animals; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000503:physiopathology*; D004815:Epidermal Growth Factor / Q000502:physiology; D066246:ErbB Receptors / Q000502:physiology*; D005784:Gene Amplification; D005786:Gene Expression Regulation; D008024:Ligands; D051379:Mice; D008970:Molecular Weight; D010455:Peptides / Q000502:physiology; D015291:Transforming Growth Factors
D008024:Ligands; D010455:Peptides; D004815:Epidermal Growth Factor; D015291:Transforming Growth Factors; D066246:ErbB Receptors