[Cholesterol in the plasmalemma of endotheliocytes in the normal aorta and in hypercholesterolemia (scanning electron microscopy research)].
Using a specific latex marker (SLM) and scanning electron microscopy, distribution of cholesterin in plasmolemma of endotheliocytes of the thoracic part of the rabbit aorta has been studied under normal conditions and at experimental atherosclerosis, as well as on the surface of liposomes and flat bilayer films. SLM reacts with a high specificity directly with cholesterin of the membrane. When studying microtopography of cholesterin, it is possible to distinguish microdomains with various density of distribution of SLM particles. Amount of SLM per 1 mcm2 of the artificial membrane surfaces grows at increase of the cholesterin-phospholipid (Ch/Phl) index of the membrane; Ch/Phl can bind with the membranes beginning only with the threshold value of the Ch/Phl index equal to 0.5. At hypercholesterolemia amount of SLM on the luminal surface of plasmolemma of the aortal endotheliocytes increases by 78%. Hence, at hypercholesterolemia in the luminal part of plasmolemma of the aortal endotheliocytes the Ch/Phl index increases; this should be taken into consideration, when observing mechanisms of reorganization of the endothelial cells layer at atherosclerosis.
Povaliĭ
T M
TM
Popova
E G
EG
Zaĭtsev
S Iu
SIu
Gusev
S A
SA
Gritskova
I A
IA
D000818:Animals; D001013:Aorta, Thoracic / Q000378:metabolism* / Q000648:ultrastructure; D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure; D002784:Cholesterol / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000378:metabolism* / Q000648:ultrastructure; D006651:Histocytochemistry; D006937:Hypercholesterolemia / Q000378:metabolism* / Q000473:pathology; D008051:Lipid Bilayers / Q000378:metabolism; D008081:Liposomes / Q000378:metabolism; D008297:Male; D008855:Microscopy, Electron, Scanning; D010743:Phospholipids / Q000378:metabolism; D011817:Rabbits
D008051:Lipid Bilayers; D008081:Liposomes; D010743:Phospholipids; D002784:Cholesterol
Kholesterin v plazmolemme éndoteliotsitov aorty v norme i pri giperkholesterinemii (skaniruiushchee élektronno-mikroskopicheskoe issledovanie).