title: Differentiation antigens on bovine mononuclear phagocytes identified by monoclonal antibodies.
abstract: Five monoclonal antibodies (MAb) produced against cell surface antigens on bovine mononuclear phagocytes (MPh) were characterized. None of the MAb recognized erythrocytes, thrombocytes, B lymphocytes or resting or activated T lymphocytes. Two MAb (IL-A22 and IL-A24) reacted with the majority of monocytes and granulocytes in peripheral blood, with 20-40% bone marrow cells comprising myelo-monocytic cells, and with a proportion of mature macrophages. Reactivity of the remaining three MAb was restricted to MPh: one of these (IL-A25) was apparently specific for pulmonary macrophages, whereas the molecules recognized by the other two (IL-A23 and CH16A) were expressed on subpopulations of blood monocytes and tissue macrophages. None of the MAb inhibited adherence of MPh to plasma-coated gelating surfaces or Fc-mediated rosette formation. One of the MAb, IL-A24, which reacts with MPh and granulocytes, inhibited antigen-specific proliferative response or peripheral blood mononuclear leukocytes (PBM) to the soluble antigen, keyhole limpet hemocyanin (KLH) but did not inhibit responses to concanavalin A or allogeneic leukocytes. This MAb was shown to react with two polypeptides of approximately 75 kD and 110 kD on the surface of peripheral blood monocytes.
authors.affiliation: International Laboratory for Research on Animal Diseases (ILRAD), Nairobi, Kenya.
authors.forename: J A
authors.initials: JA
authors.lastname: Ellis
authors.forename: W C
authors.initials: WC
authors.lastname: Davis
authors.forename: N D
authors.initials: ND
authors.lastname: MacHugh
authors.forename: D L
authors.initials: DL
authors.lastname: Emery
authors.forename: A
authors.initials: A
authors.lastname: Kaushal
authors.forename: W I
authors.initials: WI
authors.lastname: Morrison
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation / Q000276:immunology*; D002417:Cattle / Q000276:immunology*; D005260:Female; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008168:Lung / Q000276:immunology; D008264:Macrophages / Q000276:immunology; D010586:Phagocytes / Q000145:classification / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation