Interleukin 1 increases stability and transcription of mRNA encoding nerve growth factor in cultured rat fibroblasts.
The lymphokine interleukin 1 (IL-1) rapidly increases the levels of mRNA encoding nerve growth factor (NGF) in primary cultures of fibroblasts isolated from adult rat sciatic nerves. The effect of IL-1 on NGF-mRNA was not inhibited by cycloheximide, which itself increased the NGF-mRNA. From the blocking ability of actinomycin D, it was deduced that the IL-1 action depends on continuous RNA synthesis and that the half-life of NGF-mRNA is about 90 min. Nuclear run-on studies showed that, although IL-1 enhances the low basic NGF-mRNA transcription, the main effect of IL-1 results from stabilization of the NGF-mRNA. In addition, indirect evidence is presented indicating that the IL-1 effect of NGF-mRNA levels involves activation of phospholipase A2.
Lindholm
D
D
Department of Neurochemistry, Max-Planck-Institute for Psychiatry, Martinsried/Munich, Federal Republic of Germany.
Heumann
R
R
Hengerer
B
B
Thoenen
H
H
D000818:Animals; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D002478:Cells, Cultured; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology*; D007700:Kinetics; D009414:Nerve Growth Factors / Q000235:genetics*; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics*; D051381:Rats; D011994:Recombinant Proteins / Q000494:pharmacology*; D012584:Sciatic Nerve / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects*
D007375:Interleukin-1; D009414:Nerve Growth Factors; D012333:RNA, Messenger; D011994:Recombinant Proteins