Inhibition of the proliferative response of human B lymphocytes to B cell growth factor by transforming growth factor-beta.
The effects of transforming growth factor-beta (TGF-beta) on the proliferative response of human B cells to the low molecular weight B cell growth factor (BCGF) have been investigated in this study. It was found that TGF-beta, at picomolar concentrations, strongly inhibited the BCGF-induced proliferation of anti-mu chain or Staphylococcus aureus Cowan I-activated human B cells and also of a BCGF-dependent cell line derived from a human lymphocytic nodular lymphoma. This inhibitory effect was detected in normal and serum-free culture conditions. The suppression was greatly reduced when TGF-beta was added to the culture one day after BCGF and could be reverted by removing TGF-beta from the culture medium. Since TGF-beta has been detected in supernatants from activated T cells, this factor may represent an important regulatory molecule in the feedback control of B cell activation.
Petit-Koskas
E
E
U.255 INSERM, Institut Curie, Paris, France.
Génot
E
E
Lawrence
D
D
Kolb
J P
JP
D000888:Antibodies, Anti-Idiotypic / Q000502:physiology; D001402:B-Lymphocytes / Q000187:drug effects* / Q000276:immunology; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D004357:Drug Synergism; D006801:Humans; D007075:Immunoglobulin M / Q000276:immunology / Q000502:physiology; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D015847:Interleukin-4; D007378:Interleukins / Q000494:pharmacology*; D007943:Leukemia, Hairy Cell / Q000276:immunology / Q000473:pathology; D008213:Lymphocyte Activation / Q000187:drug effects*; D010455:Peptides / Q000494:pharmacology*; D015291:Transforming Growth Factors; D014407:Tumor Cells, Cultured
D000888:Antibodies, Anti-Idiotypic; D007075:Immunoglobulin M; D007166:Immunosuppressive Agents; D007378:Interleukins; D010455:Peptides; C044203:anti-IgM; D015847:Interleukin-4; D015291:Transforming Growth Factors