Liquid chromatography/mass spectrometry of fatty acids as their anilides.
The mass spectra of a series of saturated (C16:0-C30:0) and unsaturated (C16:1, C18:1, C18:2, C18:3) fatty acids have been recorded as their anilides using liquid chromatography/mass spectrometry with the atmospheric-pressure-ionization interface system. The spectra show an intense peak for (molecule + H)+ ion in each case. The liquid chromatographic/mass spectrometric separation was performed on a reverse phase column using a solvent system of methanol alone or methanol + 2-propanol. This method seemed promising for application to both qualitative and quantitative micro-analysis of fatty acids including very long chain fatty acids.
Kusaka
T
T
Biochemistry Department, Kawasaki Medical School, Okayama.
Ikeda
M
M
Nakano
H
H
Numajiri
Y
Y
D000813:Anilides; D005227:Fatty Acids / Q000032:analysis*; D005231:Fatty Acids, Unsaturated / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*
D000813:Anilides; D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated