Production of monospecific antisera by immunization with precipitin line leads to misinterpretation of immunoblot.
Immunoblotting displays an artifact in the preparation of monospecific antibodies from immunocomplexes cut out of line immunoelectrophoresis. Agarose induces the formation of antibodies towards a 68 KDa protein which is contained in commercial agarose.
Aoues
A
A
Laboratoire de Photobiologie, U.E.R. des Sciences, Mont Saint Aignan, France.
Huault
C
C
D007106:Immune Sera; D007114:Immunization; D015151:Immunoblotting; D011233:Precipitin Tests
D007106:Immune Sera