[A covalently linked complex of cytochrome P-450 with adrenodoxin. Localization of the adrenodoxin binding site of cytochrome P-450].
Cytochrome P-450scc and adrenodoxin were cross-linked with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide. The sample containing 94% of a cross-linked complex and 6% of free cytochrome P-450scc was obtained after purification on cholate-Sepharose. Cytochrome P-450scc in the cross-linked complex is not reduced in the presence of NADPH and adrenodoxin reductase, but completely preserves its high spin form in the presence of Tween-20 or pregnenolone. The use of radioactive labelled adrenodoxin, chemical cleavage of cytochrome P-450scc from the cross-linked complex by o-iodosobenzoic acid and HPLC for separation of peptides demonstrated that the cytochrome P-450scc complex with adrenodoxin was cross-linked through two amino acid sequences of cytochrome P-450scc, i.e., Leu 88-Trp108 and Leu368-Trp417.
Turko
I V
IV
Adamovich
T B
TB
Kirillova
N M
NM
Usanov
S A
SA
Chashchin
V L
VL
D000325:Adrenodoxin; D000595:Amino Acid Sequence; D001665:Binding Sites; D003432:Cross-Linking Reagents; D003577:Cytochrome P-450 Enzyme System / Q000032:analysis; D008969:Molecular Sequence Data
D003432:Cross-Linking Reagents; D000325:Adrenodoxin; D003577:Cytochrome P-450 Enzyme System
Kovalentno sshityĭ kompleks tsitokhroma P-450 s adrenodoksinom. Lokalizatsiia adrenodoksinsviayvaiushchego uchastka tsitokhroma P-450.