Reversible dependence on growth factor interleukin-3 in myeloid cells expressing temperature sensitive v-abl oncogene.
Abelson murine leukemia virus (A-MuLV) has been shown to abrogate the requirement for growth factor interleukin-3 (IL-3) in a variety of hematopoietic cell lineages by a non-autocrine mechanism. By infecting an IL-3 dependent myeloid cell line, FDC-P1, with A-MuLV containing temperature sensitive tyrosine kinase mutants of the v-abl oncogene, cell lines with temperature sensitive IL-3 independence phenotype were established. At the permissive temperature, cells expressing the ts oncogenes contained 20 fold higher levels of tyrosine-phosphorylated proteins than uninfected cells and were completely IL-3 independent. When shifted to the restrictive temperature, ts A-MuLV infected cells still contained 5 to 10 fold higher levels of phosphotyrosine but became dependent on IL-3 for growth. These results demonstrate that the maintenance of A-MuLV induced IL-3 independence requires the continuous function of the v-abl oncogene.
Kipreos
E T
ET
Department of Biology, University of California, San Diego, La Jolla 92093.
Wang
J Y
JY
D000011:Abelson murine leukemia virus / Q000235:genetics / Q000502:physiology; D000818:Animals; D002455:Cell Division; D002460:Cell Line; D007377:Interleukin-3 / Q000502:physiology*; D007951:Leukemia, Myeloid / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D009857:Oncogenes; D010766:Phosphorylation; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D013696:Temperature
D007377:Interleukin-3; D011505:Protein-Tyrosine Kinases