Recognition of Mycoplasma arthritidis membrane antigens by rats and rabbits: comparison by immunoblotting and radioimmunoprecipitation.
Sera from rats convalescing from infection with Mycoplasma arthritidis were tested for their ability to react with M. arthritidis membrane antigens by immunoblotting and radioimmunoprecipitation. The absence of metabolism-inhibition (MI) antibody activity in these sera suggested that rats might fail to recognize those membrane antigens involved in eliciting MI antibodies therefore rabbit antisera, which are strongly MI positive for M. arthritidis, were used for comparison. Antigenic recognition patterns of M. arthritidis surface and membrane antigens were not identical for rats and rabbits. The most striking and reproducible difference was the failure of rats to produce IgG antibodies against a surface antigen migrating in the 47,000-50,000 molecular weight range on SDS-polyacrylamide gels. However, rats recognized at least 2 antigens which we had previously shown to be "MI antigens", therefore the inability to express MI antibodies probably cannot be explained by their inability to recognize M. arthritidis "MI antigens".
Washburn
L R
LR
University of South Dakota School of Medicine, Department of Microbiology, Vermillion 57069.
Ramsay
J R
JR
Andrews
M B
MB
D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology; D001170:Arthritis, Infectious / Q000276:immunology*; D001345:Autoradiography; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007106:Immune Sera / Q000276:immunology; D015151:Immunoblotting; D008297:Male; D009174:Mycoplasma / Q000276:immunology*; D009175:Mycoplasma Infections / Q000276:immunology*; D011233:Precipitin Tests; D011817:Rabbits; D051381:Rats; D011917:Rats, Inbred Lew
D000942:Antigens, Bacterial; D000954:Antigens, Surface; D007106:Immune Sera