Purification of cellulases from Streptomyces strain A20 by electroendosmotic preparative electrophoresis.
Five cellulase components were identified and purified in one step from Streptomyces strain A20 using electroendosmotic preparative electrophoresis. By this procedure up to 18 mg of protein mixture could be loaded on the column, with an estimated recovery of 60-70% of total activity; activity and protein recovery could be estimated 32% and 47% respectively, if only activity peaks were considered. In comparison to other purification methods, this technique results in high protein recovery and resolution of applied samples.
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Dipartimento di Biotecnologie Agrarie, Università, Padova, Italy.
Dal Belin Peruffo
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D001426:Bacterial Proteins / Q000032:analysis; D002480:Cellulase / Q000302:isolation & purification*; D004586:Electrophoresis; D004591:Electrophoresis, Polyacrylamide Gel; D013302:Streptomyces / Q000032:analysis*
D001426:Bacterial Proteins; D002480:Cellulase