Resolution of the intermediates of de novo purine biosynthesis by ion-pair reversed-phase high-performance liquid chromatography.
An ion-pair reversed-phase high-performance liquid chromotographic procedure capable of resolving twelve of the fourteen intermediates of de novo purine biosynthesis is presented. The method utilizes isocratic elution and detection by ultraviolet light absorption. Separation of all twelve intermediates can be achieved in 90 min.
Hards
R G
RG
Eleanor Roosevelt Institute for Cancer Research, Denver, CO 80206.
Patterson
D
D
D002851:Chromatography, High Pressure Liquid; D011687:Purines / Q000032:analysis* / Q000096:biosynthesis / Q000378:metabolism; D013056:Spectrophotometry, Ultraviolet
D011687:Purines