title: Age-dependent changes in the level of a 22 KDa plasma membrane phosphoprotein in developing chick embryo brain.
abstract: In vitro phosphorylation of brain proteins of developing chick embryos showed a drastic increase in the extent of phosphorylation of a 22 KDa protein from the fourteenth day reaching a peak at seventeenth day of development; the phosphorylation of the 22 KDa protein declined afterwards. Phosphoaminoacid analysis of the 22 KDa protein indicated serine residues as targets of phosphorylation. Isoelectric focusing followed by second dimensional SDS-PAGE indicated that the 22 KDa protein had a pI value of 4.5. Polymyxin B, an inhibitor of Ca2+ and phospholipid dependent protein kinases inhibited the phosphorylation of the 22 KDa protein.
authors.affiliation: Cancer Biology Division, School of Biological Sciences, Madurai Kamaraj University, India.
authors.forename: E
authors.initials: E
authors.lastname: David
authors.forename: G
authors.initials: G
authors.lastname: Shanmugam
mesh_terms: D000375:Aging / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000196:embryology / Q000378:metabolism*; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D002642:Chick Embryo; D008565:Membrane Proteins / Q000378:metabolism; D008970:Molecular Weight; D010750:Phosphoproteins / Q000378:metabolism*; D010766:Phosphorylation; D011112:Polymyxin B / Q000494:pharmacology
chemical_list: D008565:Membrane Proteins; D010750:Phosphoproteins; D011112:Polymyxin B