title: Preparation of protein components exhibiting myosin light chain kinase activities from bovine aorta: discrepancies between its enzyme activity and actomyosin activating effect.
abstract: 1) Two protein components, 155 and 130 kDa in their electrophoretic molecular weights, respectively, were isolated in a homogeneous state from bovine aorta; they showed both the superprecipitation-inducing effect on desensitized natural actomyosin and the myosin light chain kinase (MLCK) action on gizzard myosin. 2) The superprecipitating activity of the 155 kDa component was 5 time higher than that of the 130 kDa component on the basis of equivalent MLCK activity. 3) The same procedure was applied to bovine stomach, giving rise to a 155 kDa component in a homogeneous state as in the case of aorta, but the 130 kDa component thus prepared was contaminated by higher molecular weight components. 4) If compared on the basis of equivalent MLCK activity, bovine stomach 155 kDa component showed more than 10 times higher superprecipitating activity than the fraction that contained the 130 kDa component as the main constituent. 5) The discrepancy between the superprecipitating activity and MLCK activity mentioned above was discussed in relation to the Ca2+ regulation mechanism in smooth muscle contraction. The possibility that the 130 kDa component might be a proteolytic product of the 155 kDa component was also discussed.
authors.affiliation: National Institute for Physiological Sciences, Aichi.
authors.forename: H
authors.initials: H
authors.lastname: Kuwayama
authors.forename: M
authors.initials: M
authors.lastname: Suzuki
authors.forename: R
authors.initials: R
authors.lastname: Koga
authors.forename: S
authors.initials: S
authors.lastname: Ebashi
mesh_terms: D000205:Actomyosin / Q000378:metabolism*; D000818:Animals; D001011:Aorta / Q000201:enzymology*; D002417:Cattle; D002852:Chromatography, Ion Exchange; D004789:Enzyme Activation; D009219:Myosin-Light-Chain Kinase / Q000302:isolation & purification* / Q000378:metabolism; D013270:Stomach / Q000201:enzymology*
chemical_list: D000205:Actomyosin; D009219:Myosin-Light-Chain Kinase