Production of an eosinophil chemotactic lymphokine by a monocyte-derived factor from patients with hypereosinophilia.
Peripheral OKT4-positive T lymphocytes from patients with hypereosinophilia spontaneously and selectively produced an eosinophil chemotactic factor (ECF) with chemokinetic activity. The molecular weight of the ECF was about 45,000 to 70,000. A possible mechanism of its spontaneous production by T lymphocytes was analyzed. Culture supernatants of blood monocytes from the patients showed little or no ECF activity, but they had a potency to induce the ECF production from T lymphocytes from normal donors when the cells were stimulated by the supernatants, which suggests that a monocyte-derived soluble factor (MDF) stimulated T lymphocytes to produce an ECF resembling this spontaneously produced ECF from the patients. MDF seemed to be a synthesized protein by the cells. Gel filtration indicated that molecular weight of MDF ranged between 70,000 and 100,000. MDF activity was stable at 56 degrees C for 30 min but more, supernatants of stimulated monocytes by lipopolysaccharide or silica particles failed to show ECF-producing activity, whereas they showed evident lymphocyte-activation activity. Neither recombinant IL-1 nor IL-2 had ECF and ECF-producing activity. From the present experiments, it was suggested that MDF was at least partly involved in the induction of ECF production by OKT4-positive T lymphocytes in patients with hypereosinophilia.
Muramoto
K
K
Department of Surgery, Kumamoto University Medical School, Japan.
Sakata
K
K
Miyauchi
Y
Y
Hirashima
M
M
Hayashi
H
H
D000328:Adult; D000368:Aged; D001688:Biological Products / Q000502:physiology*; D002474:Cell-Free System; D002478:Cells, Cultured; D004802:Eosinophilia / Q000276:immunology* / Q000378:metabolism; D004804:Eosinophils / Q000276:immunology*; D005260:Female; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects; D008222:Lymphokines / Q000096:biosynthesis*; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000378:metabolism*; D015846:Monokines; D013601:T-Lymphocytes / Q000276:immunology
D001688:Biological Products; D008222:Lymphokines; D015846:Monokines; C022227:eosinophil stimulating promoter