Promotion of wound collagen formation in normal and diabetic mice by quadrol.
The rate of collagen deposition in implanted polytetrafluoroethylene (PTFE) tubing in non-diabetic and streptozotocin-induced (STZ) diabetic mice was measured during 14 days post-wounding. At the time of implantation, test groups received injections of either Quadrol [N,N,N',N'-tetrakis(2-hydroxypropyl)ethylenediamine], glucan, or buffer in an area adjacent to the wound site. The accumulation of collagen in the implants of Quadrol-treated non-diabetic animals was more than 200% above control on days 8 to 11 and was 50% above control on day 14. In Quadrol-treated STZ-diabetic mice, the collagen accumulation gradually increased from 50% above control on day 8 to 200% above control on day 14. Treatment with glucan increased the collagen accumulation in normal mice 200 to 300% above control from days 8 to 11 respectively and then 30% above control on day 14. Collagen accumulation in the implants of the glucan-treated STZ-diabetic mice was similar to the control group. These results indicate that Quadrol promotes in vivo collagen synthesis and that Quadrol may be effective as a stimulator of wound healing in diabetic and non-diabetic animals.
Bhide
M V
MV
Department of Chemistry, University of Akron, OH 44325.
Dunphy
M J
MJ
Mirkopulos
N
N
Smith
D J
DJ
D000818:Animals; D003094:Collagen / Q000096:biosynthesis*; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D005029:Ethylenediamines / Q000494:pharmacology*; D005260:Female; D006909:Hydroxyproline / Q000032:analysis; D051379:Mice; D008807:Mice, Inbred BALB C; D019736:Prostheses and Implants; D013997:Time Factors; D014945:Wound Healing / Q000187:drug effects
D005029:Ethylenediamines; C010629:1,1',1'',1'''-(ethylenedinitrilo)tetra-2-propanol; D003094:Collagen; D006909:Hydroxyproline