Cytogenetic characterization of a T-cell line, ATN-1, derived from adult T-cell leukemia cells.
A T-cell line, ATN-1, was established by culturing peripheral blood mononuclear cells derived from a patient with adult T-cell leukemia/lymphoma (ATL/L). Identities of the patterns of chromosomal abnormalities, cell surface phenotypes, morphologic findings, rearrangement patterns of T-cell receptor beta chain gene, and an integration site of human T-cell leukemia virus I proviral genome indicated that ATN-1 was derived from original leukemic cells. Both ATN-1 and the original leukemic cells showed a variety of patterns of chromosomal abnormalities that include 3q-, 6q-, rearrangements involving 2q31, 7q11.2, 8q11, 8q24, 19p13.3, and also 14q11 and 14q32, where genes for the T-cell receptor alpha chain and the immunoglobulin heavy chain are located. Availability of a genuine ATL/L cell line with these chromosomal abnormalities may greatly facilitate the biologic analysis of ATL/L.
Naoe
T
T
Department of Internal Medicine, Nagoya University Branch Hospital, Japan.
Akao
Y
Y
Yamada
K
K
Utsumi
K R
KR
Koike
K
K
Shamoto
M
M
Koie
K
K
Kurosawa
Y
Y
Kato
K
K
Abe
M
M
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000032:analysis; D002460:Cell Line; D002869:Chromosome Aberrations; D025063:Chromosome Disorders; D004273:DNA, Neoplasm / Q000235:genetics; D006801:Humans; D007621:Karyotyping; D007938:Leukemia / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009693:Nucleic Acid Hybridization; D013601:T-Lymphocytes / Q000166:cytology* / Q000276:immunology / Q000648:ultrastructure
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D004273:DNA, Neoplasm