Partial characterization of a fibroblast-stimulating factor produced by cloned murine T lymphocytes.
T cells may regulate tissue fibrosis through the elaboration of soluble factors that stimulate fibroblast growth. The authors previously identified a factor produced by cloned Schistosoma mansoni antigen-specific T cells which served as a competence factor for murine fibroblasts. In the present report, they further characterize this fibroblast-stimulating factor (FsF) and differentiate it from a number of other T-cell-derived lymphokine activities. Crude supernatants from concanavalin-activated cloned T cells were fractionated by gel filtration, ion exchange, or reversed-phase high-pressure liquid chromatography. FsF has an apparent molecular weight of 17,000 and could be differentiated from colony-stimulating factor (CSF), interleukin-3 (IL-3), and interferon (IFN) on the basis of chromatographic characteristics. Highly purified or recombinant IL-2, IL-3, CSF, and IFN had no significant effect on fibroblast proliferation. Furthermore, a monoclonal anti-B-cell-stimulating factor-1 antibody only partially blocked the fibroblast proliferation induced by T-cell supernatants.
Lammie
P J
PJ
Department of Medicine, University of Pennsylvania School of Medicine, Philadelphia.
Monroe
J G
JG
Michael
A I
AI
Johnson
G D
GD
Phillips
S M
SM
Prystowsky
M B
MB
D000818:Animals; D001853:Bone Marrow / Q000187:drug effects; D001854:Bone Marrow Cells; D002455:Cell Division / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D002999:Clone Cells; D003115:Colony-Stimulating Factors / Q000494:pharmacology; D005346:Fibroblast Growth Factors / Q000032:analysis* / Q000096:biosynthesis / Q000494:pharmacology; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D007376:Interleukin-2 / Q000494:pharmacology; D007377:Interleukin-3 / Q000494:pharmacology; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D008970:Molecular Weight; D012550:Schistosoma mansoni / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism*
D003115:Colony-Stimulating Factors; D007376:Interleukin-2; D007377:Interleukin-3; D005346:Fibroblast Growth Factors