Suppression of interleukin-2-mediated T-lymphocyte blastogenesis by ovine uterine luminal protein.
Ovine uterine luminal protein (ULP) obtained from ewes on Day 14 of pregnancy suppressed blastogenesis of interleukin-2 (IL-2)-dependent T-lymphocytes. Varying concentrations of ULP (4 to 96 micrograms/ml) followed by a 1:4 dilution of human IL-2 suppressed (p less than 0.001) IL-2 blastogenesis of IL-2-dependent T-lymphocytes with mean percentage of control values ranging from 55.3 to 34.5% (44.7 to 65.5% suppression, respectively). For two experiments, IL-2 was added at varying times (zero to 4 h) after the addition of ULP to cultures. Suppression was independent of IL-2 addition time. Mean (+/- SEM) percentage of control values for combined time periods for 40 and 120 micrograms ULP/ml were 43.3 +/- 1.0 and 27.8 +/- 1.9%, respectively. In another experiment, additional IL-2 (1:2 vs. 1:4 dilution) reduced (p less than 0.01) the immunosuppressive effect of ULP. Sephacryl S-200 chromatography of ULP and the phytohemagglutinin (PHA) blastogenesis assay revealed significant immunosuppressive activity for Fractions I (greater than or equal to 248,000 Mr), III (70,000 Mr), and V (14,000 Mr). These fractions also suppressed (p less than 0.001) IL-2-mediated blastogenesis of T-lymphocytes. Results indicate that immunosuppression of PHA-treated lymphocytes was associated with an alteration of the IL-2 system.
Segerson
E C
EC
Department of Animal Science, North Carolina A & T State University, Greensboro 27411.
D000818:Animals; D002850:Chromatography, Gel; D005260:Female; D006023:Glycoproteins; D007165:Immunosuppression Therapy; D007376:Interleukin-2 / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000187:drug effects*; D011247:Pregnancy; D011506:Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D015843:Serpins; D012756:Sheep; D013601:T-Lymphocytes / Q000276:immunology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D006023:Glycoproteins; D007376:Interleukin-2; D011506:Proteins; D015843:Serpins; C029708:uterine luminal fluid proteins; D013755:Tetradecanoylphorbol Acetate