title: Screening, confirmation and quantification of boldenone sulfate in equine urine after administration of boldenone undecylenate (Equipoise).
abstract: Methods for screening by thin-layer chromatography, quantification by high-performance liquid chromatography with ultraviolet detection and confirmation by gas chromatography-mass spectrometry of boldenone sulfate in equine urine after administration of boldenone undecylenate (Equipoise) are presented. Sample work-up was done with C18 liquid-solid extraction followed by solvolytic cleavage of the sulfate ester. Confirmatory evidence of boldenone sulfate in equine urine was obtained from 2 h to 42 days following a therapeutic intramuscular dose of Equipoise. The use of 19-nortestosterone sulfate as the internal standard for quantification of boldenone sulfate is discussed.
authors.affiliation: Equine Drug Testing and Toxicology, New York State College of Veterinary Medicine, Cornell University, Ithaca, NY 14850.
authors.forename: L O
authors.initials: LO
authors.lastname: Weidolf
authors.forename: T M
authors.initials: TM
authors.lastname: Chichila
authors.forename: J D
authors.initials: JD
authors.lastname: Henion
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D008401:Gas Chromatography-Mass Spectrometry; D006736:Horses / Q000652:urine*; D013431:Sulfates / Q000652:urine; D013739:Testosterone / Q000031:analogs & derivatives* / Q000378:metabolism
chemical_list: D013431:Sulfates; D013739:Testosterone; C001371:boldenone undecylenate