[Quantitative evaluation of serum IgG subclass levels by an immunoenzyme method].
We developed a enzyme linked immunosorbent assay (ELISA) for measuring IgG subclasses concentration in serum. For this we used monoclonal antibodies. The specificity of these antibodies was evaluated with a panel of myeloma proteins belonging to the 4 IgG subclasses. The ELISA was sensitive (allowing the detection of subclasses at ng level) and accurate (inter-assay coefficient of variation of 14%). Using the WHO serum 67/97 as reference, we determined the concentration of IgG subclasses in a pool of sera. In addition concentrations were measured in 69 healthy adults to study the distribution of each IgG subclass. A good correlation (r = 0.78) was obtained between the sum of the subclasses measured by ELISA and total IgG measured by immunonephelometry.
Lambin
P
P
Laboratoire d'Immunochimie, Institut National de Transfusion Sanguine, Paris, France.
Dubarry
M
M
Charron
C
C
Herance
N
N
Defendini
E
E
D000328:Adult; D000918:Antibody Specificity; D004797:Enzyme-Linked Immunosorbent Assay / Q000592:standards; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis / Q000145:classification* / Q000592:standards; D008297:Male; D012015:Reference Standards; D012016:Reference Values
D007074:Immunoglobulin G
Estimation quantitative du taux des sous-classes d'IgG sériques par une méthode immunoenzymatique.