Evidence that multiple residues on both the alpha-helices of the class I MHC molecule are simultaneously recognized by the T cell receptor.
Single amino acid substitutions at nine different positions on the H-2Kb molecules from in vitro-mutagenized, immunologically altered, somatic cell variants were correlated with their patterns of recognition by monoclonal antibodies (MAbs) and allogeneic cytotoxic T lymphocyte (CTL) clones. While MAbs were found to detect spatially discrete, domain-specific sites, CTLs interacted simultaneously with multiple residues on the alpha 1 and alpha 2 domains of the Kb molecule. The computer graphic three-dimensional Kb model structure showed that, of the seven CTL-specific residues analyzed, six residues were located on the alpha-helical regions of the two domains. Every CTL clone was found to interact with a distinct pattern of residues composed of a specific subset of the CTL-specific residues.
Ajitkumar
P
P
Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York 10461.
Geier
S S
SS
Kesari
K V
KV
Borriello
F
F
Nakagawa
M
M
Bluestone
J A
JA
Saper
M A
MA
Wiley
D C
DC
Nathenson
S G
SG
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001483:Base Sequence; D002461:Cell Line, Transformed; D002999:Clone Cells; D006183:H-2 Antigens / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008958:Models, Molecular; D009154:Mutation; D011487:Protein Conformation; D012333:RNA, Messenger / Q000235:genetics; D011948:Receptors, Antigen, T-Cell / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology
D000911:Antibodies, Monoclonal; D006183:H-2 Antigens; C032876:H-2Kb protein, mouse; D012333:RNA, Messenger; D011948:Receptors, Antigen, T-Cell