Molecular weight of tumor necrosis factor determined by gel permeation chromatography alone or in combination with low-angle laser light scattering.
The molecular weight of human recombinant tumor necrosis factor was determined at neutral pH by gel permeation chromatography alone or in combination with low-angle laser light scattering. Mean values of 39,200 +/- 800 and 48,800 +/- 900, respectively, were obtained by the two analyses. The results resolve the apparent discrepancy in the reported values of the molecular weight of this cytokine, confirming that it exists as a trimer in neutral solution with a molecular weight of about 50,000.
Yoshimura
T
T
Institute for Enzyme Research, School of Medicine, University of Tokushima, Japan.
Sone
S
S
Maezawa
S
S
Kameyama
K
K
Takagi
T
T
D002850:Chromatography, Gel; D007834:Lasers; D008027:Light; D008970:Molecular Weight; D012542:Scattering, Radiation; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis*
D014409:Tumor Necrosis Factor-alpha