Ultrastructural study of the semicircular canal cells of the frog Rana esculenta.
The ultrastructure of the nonsensory cells (dark cells, transitional cells, and undifferentiated cells) of the frog semicircular canal was studied by using transmission electron microscopy in an attempt to correlate the structure with the functions of these epithelial cells. All the nonsensory cells were linked by tight junctions and desmosomes; this suggested that there is little paracellular ionic transport from perilymph to endolymph. In the dark cell epithelium, the apical intercellular spaces were dilated; in the basal part, numerous basolateral plasma membrane infoldings, containing mitochondria, delimited electron-lucent spaces. The undifferentiated cells and the transitional cells were devoid of any basal membrane infolding. Surrounding the semicircular canal, very flattened and interdigitated mesothelial cells constituted a thin multilayer tissue which limited the perilymphatic space. The morphological aspect of the dark cells suggests that they may play a role in the secretion and/or in the reabsorption of endolymph, which bathes the apical pole of these cells. The undifferentiated and transitional cells can play a role in the maintenance of the endolymphatic ionic composition because of their apical tight junctions and desmosomes.
Oudar
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Département de Physiologie and INSERM U.251, Faculté de Médecine Xavier Bichat, Université Paris, France.
Ferrary
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E
Feldmann
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D000818:Animals; D004710:Endolymph / Q000378:metabolism; D004848:Epithelium / Q000648:ultrastructure; D007365:Intercellular Junctions / Q000648:ultrastructure; D011893:Rana esculenta / Q000033:anatomy & histology*; D012665:Semicircular Canals / Q000502:physiology / Q000648:ultrastructure*