title: Electrical properties and fluxes of Na+ and Cl- across lizard intestine.
abstract: Electrical parameters and unidirectional Na+ and Cl- fluxes were determined in vitro across the duodenum, ileum and colon of lizard (Gallotia galloti). Electrical potential difference (PD) and short circuit current (Isc) were low in the three segments studied, whilst tissue conductance (Gt) was high. A net active transport of Na+ and Cl- was observed in the three segments. Net Na+ absorption was higher across duodenum and ileum than across the colon, while net Cl- absorption was similar in duodenum, ileum and colon. Ouabain virtually abolished Isc, PD and net Na+ and Cl- fluxes in all the segments. Amiloride abolished net Cl- flux in duodenum, ileum and colon, whereas net Na+ flux was abolished in colon but decreased in duodenum and ileum. PD and Isc were not affected by the presence of the diuretic.
authors.affiliation: Departamento de Biología Animal, Facultad de Biología, Universidad de La Laguna, Tenerife, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Lorenzo
authors.forename: T
authors.initials: T
authors.lastname: Gómez
authors.forename: P
authors.initials: P
authors.lastname: Badía
authors.forename: A
authors.initials: A
authors.lastname: Bolaños
mesh_terms: D000584:Amiloride / Q000494:pharmacology; D000818:Animals; D001693:Biological Transport, Active; D002712:Chlorides / Q000378:metabolism*; D005260:Female; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000502:physiology*; D008116:Lizards; D008297:Male; D008564:Membrane Potentials; D010042:Ouabain / Q000494:pharmacology; D012964:Sodium / Q000378:metabolism*
chemical_list: D002712:Chlorides; D010042:Ouabain; D000584:Amiloride; D012964:Sodium