Encapsulation of interleukin-2 in murine erythrocytes and subsequent deposition in mice receiving a subcutaneous injection.
Radiolabeled recombinant human interleukin-2 (IL-2) was successfully encapsulated in both mouse and sheep erythrocytes. Of the added IL-2, 70% was recovered bound to or encapsulated within the carrier cells. Erythrocytes containing IL-2 were stable in vitro and most of the IL-2 remained associated with the cells following a 16-h incubation at 37 degrees C. When carrier erythrocytes containing IL-2 were injected subcutaneously into mice, intact [35S]IL-2 was detectable in a number of tissues 3 days after injection.
DeLoach
J R
JR
Veterinary Toxicology and Entomology Research Laboratory, U.S. Department of Agriculture, P.O., College Station, Texas 77841.
Andrews
K
K
Sheffield
C L
CL
D000818:Animals; D004912:Erythrocytes; D006801:Humans; D007279:Injections, Subcutaneous; D007376:Interleukin-2 / Q000008:administration & dosage* / Q000493:pharmacokinetics; D051379:Mice; D008807:Mice, Inbred BALB C; D011994:Recombinant Proteins / Q000008:administration & dosage* / Q000493:pharmacokinetics; D013462:Sulfur Radioisotopes; D014018:Tissue Distribution
D007376:Interleukin-2; D011994:Recombinant Proteins; D013462:Sulfur Radioisotopes