Induction of T cell serine proteinase 1 (TSP-1)-specific mRNA in mouse T lymphocytes.
An oligonucleotide probe corresponding to nucleotides of a cDNA encoding the T cell-associated proteinase 1 (TSP-1) was chosen to study the induction and expression of TSP-1-specific transcripts in mouse T lymphocytes and tissues. We demonstrate that TSP-1 mRNA is only expressed in activated T lymphocytes and is absent from all mouse tissues tested including those containing resting mature T lymphocytes. Expression of the TSP-1 gene was observed in T lymphocytes in vitro in response to either phorbolester (phorbol 12-myristate 13-acetate), Ca2+ ionophore (A23187), lectin or alloantigen. In general, TSP-1 mRNA appeared and peaked later compared to interleukin 2 transcripts. Furthermore, TSP-1 mRNA was inducible in vitro in both Ly-2+ and L3T4+ lymphocyte populations treated with alloantigen and/or lectin. The transcription of the TSP-1 gene was always accompanied by the expression of proteinase activity. High expression of TSP-1 transcripts was also observed in in vivo derived T effector cells specific for lymphocytic choriomeningitis virus. However, TSP-1 mRNA was predominantly associated with virus-specific Ly-2+ T cells and correlated with their proteinase and cytolytic activities. The data suggest that TSP-1 gene transcription is a useful marker to characterize T effector cells in vitro and in vivo.
Simon
H G
HG
Max-Planck-Institut für Immunobiologie, Freiburg, FRG.
Fruth
U
U
Eckerskorn
C
C
Lottspeich
F
F
Kramer
M D
MD
Nerz
G
G
Simon
M M
MM
D000595:Amino Acid Sequence; D000818:Animals; D000950:Antigens, Ly; D001483:Base Sequence; D004790:Enzyme Induction; D005260:Female; D008213:Lymphocyte Activation; D008217:Lymphocytic choriomeningitis virus / Q000276:immunology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008969:Molecular Sequence Data; D009841:Oligonucleotides; D012333:RNA, Messenger / Q000096:biosynthesis*; D012697:Serine Endopeptidases / Q000096:biosynthesis* / Q000302:isolation & purification; D013601:T-Lymphocytes / Q000145:classification / Q000201:enzymology* / Q000276:immunology
D000950:Antigens, Ly; D009841:Oligonucleotides; D012333:RNA, Messenger; D012697:Serine Endopeptidases