Bistramide A, a new toxin from the urochordata Lissoclinum bistratum Sluiter: isolation and preliminary characterization.
Two cases of human intoxication caused by the lyophilized powder of Lissoclinum bistratum Sluiter, a New Caledonian ascidian, are reported. The symptoms observed were caused by a substance designated bistramide A (C40H68N2O8) of hitherto unknown chemical structure. Preliminary toxicological investigations indicate that bistramide A may effect the central nervous system, leading to paresthesia and loss of muscle tone. A progressive decrease in cardiac rhythm was also observed in animals. Bistramide A (1.4 x 10(-6) M) did not alter the resting potential of frog heart and skeletal muscle but reduced the amplitude and duration of cardiac action potential and prolonged the interval between action potentials. Bistramide A also has a marked cytotoxic effect on cancer cells KB (IC50 = 4.5 x 10(-8) M) and P 388 (IC50 = 2.0 x 10(-8) M) and on normal endothelial cells (IC50 = 2.2 x 10(-8) M). However, it has not been possible to relate the cytotoxic property to the symptoms of intoxication. Bistramide A may originate from the urochordate itself or from symbiotic algae.
Gouiffes
D
D
SMAB (Substances Marines à Activité Biologique), UER des Sciences Pharmaceutiques, Nantes, France.
Juge
M
M
Grimaud
N
N
Welin
L
L
Sauviat
M P
MP
Barbin
Y
Y
Laurent
D
D
Roussakis
C
C
Henichart
J P
JP
Verbist
J F
JF
D000081:Acetamides; D000818:Animals; D001156:Artemia / Q000187:drug effects; D004594:Electrophysiology; D004988:Ethers, Cyclic / Q000302:isolation & purification / Q000633:toxicity; D007928:Lethal Dose 50; D051379:Mice; D011714:Pyrans; D051381:Rats; D013141:Spiro Compounds; D014118:Toxins, Biological / Q000032:analysis / Q000302:isolation & purification / Q000633:toxicity*; D014561:Urochordata
D000081:Acetamides; D004988:Ethers, Cyclic; D011714:Pyrans; D013141:Spiro Compounds; D014118:Toxins, Biological; C059975:bistratene A