title: Spectrophotometric determination of isocarboxazid bulk drug and tablets by reaction with p-dimethylaminocinnamaldehyde.
abstract: A spectrophotometric method is described for the determination of isocarboxazid. The method is based on the reaction of the drug with p-dimethylaminocinnamaldehyde in the presence of trichloroacetic acid in a methanolic medium to produce a very intense red chromophore (lambda max = 500 nm, Emax = 1.05 x 10(5]. The reaction is proposed to proceed via electrophilic attack at the C-4 position of the isoxazole nucleus. Job's plot indicated a 1:1 drug-to-reagent ratio. Regression analysis of Beer's plot showed excellent correlation (r = 0.9996) in the concentration range 0.25-2.10 micrograms isocarboxazid/mL. The developed color is stable for at least 12 h. Results of analyses of bulk drug and tablets by the proposed method are comparable to those for USP XXI methods.
authors.affiliation: University of Assiut, Faculty of Pharmacy, Pharmaceutical Chemistry Department, Egypt.
authors.forename: M E
authors.initials: ME
authors.lastname: el-Kommos
mesh_terms: D002384:Catalysis; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002934:Cinnamates / Q000032:analysis; D007490:Iproniazid / Q000032:analysis; D007520:Isocarboxazid / Q000032:analysis*; D007538:Isoniazid / Q000032:analysis; D011208:Powders; D013055:Spectrophotometry, Infrared; D013607:Tablets
chemical_list: D002934:Cinnamates; D011208:Powders; D013607:Tablets; D007520:Isocarboxazid; C018523:4-dimethylaminocinnamaldehyde; D007490:Iproniazid; D007538:Isoniazid