In vitro induction of T cell-dependent B cell differentiation in patients with common varied immunodeficiency.
Patients with common varied immunodeficiency (CVI) are characterized by hypogammaglobulinemia. We investigated in vitro T cell-dependent B cell differentiation in CVI peripheral blood mononuclear cells by stimulating the T cells with an anti-CD3 (T3) monoclonal antibody. In cultures from CVI patients with no detectable circulating B cells, little immunoglobulin (Ig) was produced following anti-CD3 stimulation. In cultures from CVI patients with near-normal numbers of circulating B cells, anti-CD3 stimulation induced a normal percentage increase in Ig-secreting cells and appreciable (albeit subnormal) increases in IgG and IgM secretion. Cell-mixing experiments pointed to a quantitative, rather than qualitative, defect in B cell function in most of these CVI patients. Nevertheless, CVI T cells can induce substantial differentiation of autologous (and normal) B cells following anti-CD3 stimulation (which may mimic physiologic stimulation). This raises the possibility of correcting the hypogammaglobulinemia of CVI by in vivo or ex vivo administration of appropriate T cell stimuli.
Stohl
W
W
Rockefeller University, New York 10021.
Cunningham-Rundles
C
C
Mayer
L
L
D000293:Adolescent; D000328:Adult; D000361:Agammaglobulinemia / Q000276:immunology* / Q000473:pathology; D000911:Antibodies, Monoclonal; D000917:Antibody Formation; D000938:Antigen-Presenting Cells / Q000276:immunology; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology; D000954:Antigens, Surface; D001402:B-Lymphocytes / Q000145:classification / Q000276:immunology* / Q000473:pathology; D002454:Cell Differentiation; D005260:Female; D006801:Humans; D007519:Isoantigens / Q000276:immunology; D008213:Lymphocyte Activation; D008297:Male; D010641:Phenotype; D013601:T-Lymphocytes / Q000145:classification / Q000276:immunology* / Q000473:pathology
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface; D007519:Isoantigens