title: Spontaneous acute lymphoblastic leukemia in Sprague-Dawley rats. II. Cytogenetic analysis of nine individual leukemias.
abstract: Nine spontaneous acute lymphoblastic leukemias (SD1-SD9) in Sprague-Dawley rats were investigated cytogenetically by G-banding. The chromosome numbers of metaphase cells in all studied SD leukemias were near diploid and in all leukemias numerical or structural abnormalities were found. Affected were chromosomes No. 11, 2, 13 and 1. A common finding in most leukemias was trisomy 11 observed either as simple trisomy (SD1, SD4 and 2 clones of SD6), or the translocation form of trisomy (SD3), or the tandem translocation t(11;13) in SD7, or the Robertsonian fusion -rob(2;11) in SD5. In SD9 and one cell clone of SD8 no apparent trisomy was found, but the cells contained an 11q+ marker. Among the rearranged chromosomes, chromosome 2 was most frequently involved. Der No. 2 with terminal deletion was found in some of SD3 metaphases and a probable partial duplication of chromosome 2 was found in SD6. The finding of trisomy 2 in SD5 and SD6 occurred rarely. Further structural rearrangements concerned No. 13, the involvement of which was evident in the 13q+ marker typical of SD2, one cell clone of SD6, SD7 and SD8 leukemias. 1q+ aberration was the common finding in SD3 and SD5, while 1q-was observed in all SD6 metaphases. According to the cytogenetic examination of SD leukemias, we considered that the changes of chromosomes 11, 2, 13 and 1 were nonrandom. Based on the similarity of chromosomal rearrangements in individual leukemias, probable breakpoints on the affected chromosomes could be determined. 11cen, 11q12, 2q32-33, 2q16, 13q22, 1q43 and 1q54 were found to be the most frequently afflicted regions in SD leukemias.
authors.affiliation: Department of Biology, Faculty of Medicine, Prague, Czechoslovakia.
authors.forename: M
authors.initials: M
authors.lastname: Sladká
authors.forename: V
authors.initials: V
authors.lastname: Kren
authors.forename: P
authors.initials: P
authors.lastname: Klír
mesh_terms: D000782:Aneuploidy; D000818:Animals; D002869:Chromosome Aberrations; D002874:Chromosome Mapping; D004171:Diploidy; D005260:Female; D007621:Karyotyping; D007942:Leukemia, Experimental / Q000235:genetics*; D008297:Male; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics*; D051381:Rats; D011917:Rats, Inbred Lew; D011919:Rats, Inbred Strains; D012730:Sex Chromosomes