Identification of bile alcohols in serum from healthy humans.
Bile alcohol glucuronides present in human serum were isolated by ion exchange chromatography on piperidino-hydroxypropyl Sephadex LH-20. Following hydrolysis with beta-glucuronidase, the bile alcohols were analyzed by a combination of gas-liquid chromatography and mass spectrometry. Bile alcohols identified were 27-nor-5 beta-cholestane-3 alpha,7 alpha,12 alpha,24,25-pentol, 27-nor-5 beta-cholestane-3 alpha,7 alpha,12 alpha,24,25,26-hexol, 5 beta-cholestane-3 alpha,7 alpha,12 alpha,24,25-pentol, 5 beta-cholestane-3 alpha,7 alpha,12 alpha,24,26-pentol, 5 beta-cholestane-3 alpha,7 alpha,12 alpha,25,26-pentol, and 5 beta-cholestane-3 alpha,7 alpha,12 alpha,24,25,26-hexol. The bile alcohol composition in serum was similar to that in urine but not to that in bile. The concentration of total bile alcohols in serum was 59.5 +/- 24.6 micrograms/L.
Hiraoka
T
T
First Department of Internal Medicine, Hiroshima University School of Medicine, Japan.
Kihira
K
K
Kosaka
D
D
Kohda
T
T
Hoshita
T
T
Kajiyama
G
G
D000328:Adult; D001646:Bile / Q000378:metabolism; D002777:Cholestanols / Q000097:blood* / Q000302:isolation & purification; D002852:Chromatography, Ion Exchange; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007668:Kidney / Q000378:metabolism; D008297:Male
D002777:Cholestanols