title: Destruction of ethidium bromide in solution by ozonolysis.
abstract: Ethidium bromide can be rapidly destroyed in aqueous solutions or in isoamyl alcohol by ozonolysis in the presence of H2O2 to give a mixture of organic acids. In a variety of buffers commonly used in recombinant DNA technology destruction of ethidium bromide was more than 99.9%. The yellow reaction mixture after ozonolysis was shown to be nonmutagenic. This method may be used in laboratories for the disposal of ethidium bromide wastes.
authors.affiliation: Institut für Biochemie und Molekulare Biologie der Technischen Universität Berlin.
authors.forename: R
authors.initials: R
authors.lastname: Zocher
authors.forename: A
authors.initials: A
authors.lastname: Billich
authors.forename: U
authors.initials: U
authors.lastname: Keller
authors.forename: P
authors.initials: P
authors.lastname: Messner
mesh_terms: D055598:Chemical Phenomena; D002621:Chemistry; D004996:Ethidium; D006861:Hydrogen Peroxide; D007700:Kinetics; D010126:Ozone; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet
chemical_list: D012996:Solutions; D010126:Ozone; D006861:Hydrogen Peroxide; D004996:Ethidium