Influence of flow and diffusion on protein separation in a continuous flow electrophoresis cell: computation procedure.
Continuous flow zone electrophoresis is an efficient method for the non-destructive separation of biological materials in a flowing film of buffer solution in which the constituents of a sample are separated according to their electrophoretic mobilities, under the influence of an electric field. This paper deals with modeling of flow structure of the buffer solution taking into account the effect of electroosmosis, Joule heating and thermal free convection and the forced convection of axial flow. Modeling of diffusion is examined in a second part. The modeling equations and the corresponding boundary conditions are solved by finite difference methods. The various parameters affecting the quality of the fractionation are analyzed.
Biscans
B
B
Laboratoire de Génie Chimique, UA, Toulouse, France.
Alinat
P
P
Bertrand
J
J
Sanchez
V
V
D004058:Diffusion; D004586:Electrophoresis; D008433:Mathematics; D011506:Proteins / Q000302:isolation & purification*
D011506:Proteins