Preparative separation of human B and T lymphocytes by free flow electrophoresis.
An electrophoretic method for the quantitative separation of human B and T lymphocytes in a carrier-free system is presented. The method is based on the fact that B and T lymphocytes show marked overlap in their size and density characteristics, but differ sufficiently in surface charge to be separable by electrophoresis. The technique is performed in phosphate-buffered saline and appears to be especially suitable for the enrichment of nonstimulated, functionally intact lymphocytes which can be directly used for further immunological or biochemical studies.
Baier
T G
TG
Department of Pediatrics, University of Heidelberg, Federal Republic of Germany.
Weber
G
G
Hartmann
K
K
Heinrich
U
U
Schönberg
D
D
D001402:B-Lymphocytes; D002452:Cell Count; D002469:Cell Separation; D002499:Centrifugation, Density Gradient; D004586:Electrophoresis / Q000379:methods; D006801:Humans; D007963:Leukocytes, Mononuclear; D013499:Surface Properties; D013601:T-Lymphocytes