Possible association between IL-2 receptors and class I HLA molecules on T cells.
In the process of evaluating murine hybridomas for an antibody to the beta-subunit of the IL-2R (p70) we identified an antibody that immunoprecipitated a 55- to 57-kDa complex from cross-linked lysates. We demonstrate that this complex is composed of IL-2 (15.5 kDa) cross-linked to the H chain of HLA class I (40 to 42 kDa), suggesting a molecular interaction between HLA class I molecules and IL-2R. Although the exact role of this association remains to be determined, the specific cross-linking of IL-2 to HLA class I Ag is intriguing in view of published claims for a role of HLA class I in OKT3-induced lymphocyte proliferation and in NK cell lytic activity.
Sharon
M
M
Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, Bethesda, MD 20892.
Gnarra
J R
JR
Baniyash
M
M
Leonard
W J
WJ
D000818:Animals; D000911:Antibodies, Monoclonal; D002460:Cell Line; D003432:Cross-Linking Reagents; D005260:Female; D006680:HLA Antigens / Q000302:isolation & purification; D015395:Histocompatibility Antigens Class I / Q000302:isolation & purification; D006801:Humans; D006825:Hybridomas / Q000032:analysis; D051379:Mice; D008807:Mice, Inbred BALB C; D011233:Precipitin Tests; D015375:Receptors, Interleukin-2 / Q000302:isolation & purification; D013601:T-Lymphocytes / Q000032:analysis*
D000911:Antibodies, Monoclonal; D003432:Cross-Linking Reagents; D006680:HLA Antigens; D015395:Histocompatibility Antigens Class I; D015375:Receptors, Interleukin-2