Separation of neutral reducing oligosaccharides derived from glycoproteins by HPLC on a hydroxylated polymeric support.
A prototype hydroxylated polymeric support, Glyco-PAK N, was employed to separate a number of neutral reducing oligosaccharides derived from various glycoproteins by the hydrazinolysis/re-N-acetylation/mild acid hydrolysis procedure. General features of the separation properties of the matrix are described. A major advantage of the packing over silica-based supports is its stability, a property which is advantageous both in repetitive analytical chromatography and in the preparative isolation and spectroscopic identification of pure compounds, free of column breakdown products.
Bendiak
B
B
Department of Biochemistry, Hospital for Sick Children, Toronto, Ontario, Canada.
Orr
J
J
Brockhausen
I
I
Vella
G
G
Phoebe
C
C
D000097:Acetonitriles; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006023:Glycoproteins / Q000302:isolation & purification*; D006868:Hydrolysis; D006900:Hydroxylation; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000302:isolation & purification*
D000097:Acetonitriles; D006023:Glycoproteins; D009844:Oligosaccharides