title: A new cell line, GS, derived from a human renal cell carcinoma.
abstract: A new cell line, GS, of human renal cell carcinoma was established and characterized. It was derived from a metastasis of a human renal cell carcinoma, which appeared 6 years after nephrectomy. The GS cells exhibit basic characteristics of renal cell carcinoma: epithelial cell character, PAS and glycogen positivity, typical ultrastructural features. The cells have a pseudotriploid stemline with a modal number of 75 chromosomes and two marker chromosomes. GS cells formed neither colonies in soft agar nor transplantable tumours in nude mice but produced a factor(s) stimulating growth and colony forming activity of indicator cells.
authors.affiliation: Oncological Department, Therapeutical Institute Ples.
authors.forename: J
authors.initials: J
authors.lastname: Vydra
authors.forename: R
authors.initials: R
authors.lastname: Vytásek
authors.forename: H
authors.initials: H
authors.lastname: Sainerová
authors.forename: V
authors.initials: V
authors.lastname: Mandys
authors.forename: V
authors.initials: V
authors.lastname: Sovová
authors.forename: J
authors.initials: J
authors.lastname: Hejnar
authors.forename: E
authors.initials: E
authors.lastname: Sloncová
authors.forename: P
authors.initials: P
authors.lastname: Poucková
authors.forename: J
authors.initials: J
authors.lastname: Bubeník
authors.forename: J
authors.initials: J
authors.lastname: Nováak
mesh_terms: D000368:Aged; D000818:Animals; D002277:Carcinoma; D002455:Cell Division; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D006801:Humans; D007621:Karyotyping; D007680:Kidney Neoplasms; D008297:Male; D051379:Mice; D008819:Mice, Nude; D013997:Time Factors; D014407:Tumor Cells, Cultured / Q000166:cytology / Q000378:metabolism*