Early appearance of activated CD4+ T lymphocytes and class II antigen-expressing cells in joints of DBA/1 mice immunized with type II collagen.
Arthritis induced with type II collagen in DBA/1 mice, was analyzed by immunohistochemical techniques. In the earliest detectable pathologic changes, before any macroscopic signs, an accumulation of Mac1+, macrophage-like cells, and an increased expression of major histocompatibility class II antigens were observed focally in the synovial lining layer. In these foci, CD4+ and interleukin 2 receptor expressing T lymphocytes were regularly detected, but not usually other sets of lymphocytes such as B lymphocytes and CD8+ lymphocytes. In clinically detectable arthritis, there was a prominent infiltration of Mac1+ cells, both polymorphonuclear-like and macrophage-like cells. T cells were relatively few, suggesting that they do not play a primary effector role, but rather that they may regulate or permit the self-perpetuative inflammation.
Holmdahl
R
R
Department of Medical and Physiological Chemistry, University of Uppsala, Sweden.
Jonsson
R
R
Larsson
P
P
Klareskog
L
L
D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis*; D001168:Arthritis / Q000473:pathology*; D001169:Arthritis, Experimental / Q000209:etiology / Q000378:metabolism / Q000473:pathology*; D003094:Collagen / Q000008:administration & dosage* / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000032:analysis*; D007150:Immunohistochemistry; D007249:Inflammation / Q000209:etiology / Q000378:metabolism / Q000473:pathology; D007596:Joints / Q000032:analysis / Q000473:pathology; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008811:Mice, Inbred DBA; D013601:T-Lymphocytes / Q000276:immunology / Q000473:pathology*; D013997:Time Factors
D000945:Antigens, Differentiation, T-Lymphocyte; D000949:Histocompatibility Antigens Class II; D003094:Collagen