Determination of a novel antiarrhythmic agent ACC-9358 in human plasma by high-performance liquid chromatography.
A liquid chromatographic method for the determination of ACC-9358, an antiarrhythmic agent, in human plasma is described. ACC-9358 was extracted from plasma into chloroform and back extracted into 0.01 N hydrochloric acid. ACC-9358 in the acidic extract was eluted from a Novapak C18 column using a 87:13:0.1 (by volume) mixture of phosphate buffer (pH 3.0), acetonitrile, and triethylamine as the mobile phase and measured at 280 nm. The average extraction recoveries were 78.4 and 85.4% at the 0.5- and 5-micrograms/ml level, respectively. Standard curves were linear and reproducible over the concentration range of 0.01-10 micrograms/ml, with coefficients of determination better than 0.999. Coefficients of variation for both within-day and day-to-day analysis were less than 11%. The assay method is sensitive, reproducible, and suitable for disposition studies of ACC-9358 in humans.
Achari
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Pharmaceutical Research Department, Du Pont Critical Care, Waukegan, Illinois 60085.
Thomas
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D000889:Anti-Arrhythmia Agents / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D004355:Drug Stability; D006801:Humans; D011759:Pyrrolidines / Q000097:blood*; D015203:Reproducibility of Results; D012996:Solutions
D000889:Anti-Arrhythmia Agents; D011759:Pyrrolidines; D012996:Solutions; C048492:4-hydroxy-N-phenyl-3,5-bis(1-pyrrolidinylmethyl)benzamide