An analysis by chemical class of Salmonella mutagenicity tests as predictors of animal carcinogenicity.
For a number of years, investigators have recognized that humans potentially are exposed to large numbers of genotoxicants. Many efforts have attempted to validate various short-term bioassays for use as rapid, inexpensive screens for genotoxicants--especially carcinogens. In this analysis, we examine Salmonella mutagenicity as an indicator of potential carcinogenicity by comparing published (and when possible, evaluated) Salmonella results with the evaluated Gene-Tox animal carcinogen data base. The Salmonella bioassay does especially well in those cases where the level of evidence for carcinogenicity is the strongest. Analysis shows that except for specific classes of compounds, the plate-incorporation protocol and the preincubation protocol are equally efficient at detecting mutagens. This paper also demonstrates how validation values (sensitivity, specificity, etc.) vary with chemical class. Overall, this analysis demonstrates that when used and interpreted in a meaningful chemical class context, the Salmonella bioassay remains extremely useful in identifying potential animal carcinogens.
Claxton
L D
LD
Health Effects Research Laboratory, U.S. Environmental Protection Agency, Research Triangle Park, NC 27711.
Stead
A G
AG
Walsh
D
D
D000818:Animals; D001681:Biological Assay; D002273:Carcinogens; D066298:In Vitro Techniques; D007256:Information Systems; D009152:Mutagenicity Tests / Q000379:methods / Q000592:standards*; D009374:Neoplasms, Experimental / Q000139:chemically induced; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics*; D013329:Structure-Activity Relationship
D002273:Carcinogens