Genetics of the induction of antigen-specific immunity in anti-idiotype-treated mice and derivation of monoclonal Ab3 antibodies.
Antiidiotypic antibodies can be used as a means other than antigen of clone-specific intervention in the immune system. In a number of cases, antiidiotypic antibodies induce potent, antigen-specific responses without exposure of the recipient animals to antigen, and a better understanding of such responses will aid in the application of idiotype manipulation to both immunological and practical problems. The Ia. 7 system expresses a major, cross-reactive idiotype on antibodies to an epitope of the mouse I-E antigen. Here, we examine genetic control of the I-E-specific response to anti-idiotype in this model system. Results demonstrate that both heavy chain allotype-linked and non-allotype-linked loci contribute to the control of this response. In addition, monoclonal antibodies representing the non-antigen-specific portion of the Ab3 response were derived. Two idiotype-positive clones were demonstrated to be of IgM isotype, contrasting with previous data showing that the serum Ab3 response is predominantly IgG1.
Epstein
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SL
Division of Biochemistry and Biophysics, FDA, Bethesda, MD.
Sharrow
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SO
Sachs
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DH
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D007132:Immunoglobulin Isotypes / Q000276:immunology; D007518:Isoantibodies / Q000235:genetics*; D051379:Mice; D008815:Mice, Inbred Strains; D013045:Species Specificity
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D000949:Histocompatibility Antigens Class II; D007130:Immunoglobulin Idiotypes; D007132:Immunoglobulin Isotypes; D007518:Isoantibodies