Cooperative thermal denaturation of the assembly origin region of TMV RNA.
The assembly origin (AO) region of the tobacco mosaic virus RNA melts in an usually narrow (2.5 degrees C) temperature range. In an 0.01 M phosphate buffer the melting temperature of AO was found to be 41.5 degrees C. This value corresponds to the regions with the most stable secondary/tertiary structure of the whole TMV RNA molecule. It is assumed that the AO region has a specific tertiary structure, which is maintained by the long-range interactions as well as by interactions of the pseudoknot type.
Kelve
M
M
Institute of Experimental Biology Academy of Sciences, Estonia, USSR.
Aruja
A
A
Kooli
K
K
Männik
J
J
Raukas
E
E
D001483:Base Sequence; D005814:Genes, Viral; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation / Q000187:drug effects; D009691:Nucleic Acid Denaturation / Q000187:drug effects; D009994:Osmolar Concentration; D012367:RNA, Viral / Q000187:drug effects / Q000648:ultrastructure*; D012045:Regulatory Sequences, Nucleic Acid; D012965:Sodium Chloride / Q000494:pharmacology; D013696:Temperature; D014027:Tobacco Mosaic Virus / Q000235:genetics
D012367:RNA, Viral; D012965:Sodium Chloride