Virus-specific memory T cells are Pgp-1+ and can be selectively activated with phorbol ester and calcium ionophore.
Memory lymphocytic choriomeningitis virus (LCMV)-immune cytotoxic T-lymphocyte precursors (CTLp) can be stimulated to proliferate and to mediate specific cytotoxic activity following incubation with phorbol myristate acetate (PMA), calcium ionophore (CaI), and interleukin 2 (IL-2). This protocol can be used to selectively induced virus-specific CTL activity under both bulk culture and limiting dilution conditions, in the absence of added antigen. There is no concurrent stimulation of alloreactive CTLp. Proliferation of the effector Lyt-2+ population in medium containing PMA and CaI requires L3T4+ cells, which can be replaced by adding IL-2, and the development of cytotoxicity is totally IL-2 dependent. The LCMV-specific memory T cells are also characterized by the expression of the Pgp-1 (Ly24) glycoprotein. The availability of this marker, together with the capacity to selectively stimulate primed CTLp in the absence of antigen, should greatly facilitate the analysis of T-cell memory in virus infections.
Tabi
Z
Z
Department of Experimental Pathology, John Curtin School of Medical Research, Canberra, Australia.
Lynch
F
F
Ceredig
R
R
Allan
J E
JE
Doherty
P C
PC
D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis*; D000954:Antigens, Surface / Q000032:analysis*; D003602:Cytotoxicity, Immunologic; D004987:Ethers / Q000494:pharmacology; D007156:Immunologic Memory; D066298:In Vitro Techniques; D007376:Interleukin-2 / Q000494:pharmacology; D015759:Ionomycin; D008217:Lymphocytic choriomeningitis virus / Q000276:immunology; D051379:Mice; D016201:Receptors, Lymphocyte Homing; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface; D004987:Ethers; D007376:Interleukin-2; D016201:Receptors, Lymphocyte Homing; D015759:Ionomycin; D013755:Tetradecanoylphorbol Acetate