Variation of electrophoretic karyotypes among clinical isolates of Candida albicans.
Orthogonal-field-alternation gel electrophoresis was used to compare clinical isolates of Candida albicans by resolving chromosome-sized DNA molecules into an electrophoretic karyotype. Seven to nine bands were observed among isolates recovered from 17 patients. In addition, 14 distinct electrophoretic patterns were noted among the isolates from these patients. In a given individual, isolates were likely to have identical electrophoretic patterns. Therefore, the electrophoretic karyotype patterns demonstrated by orthogonal-field-alternation gel electrophoresis can be used to designate a strain for epidemiologic studies.
Merz
W G
WG
Department of Laboratory Medicine (Pathology), Johns Hopkins Medical Institutions, Baltimore, Maryland 21205.
Connelly
C
C
Hieter
P
P
D002176:Candida albicans / Q000145:classification / Q000235:genetics*; D004271:DNA, Fungal / Q000032:analysis*; D004587:Electrophoresis, Agar Gel; D004996:Ethidium; D014644:Genetic Variation; D006801:Humans; D007621:Karyotyping; D012441:Saccharomyces cerevisiae / Q000235:genetics
D004271:DNA, Fungal; D004996:Ethidium