Polyamine metabolism and interconversion in NIH 3T3 and ras-transfected NIH 3T3 cells.
The effect of transfection of NIH 3T3 cells by the human ras (c-Ha-ras-1) oncogene on uptake, interconversion, and excretion of polyamines was studied. Uptake and interconversion of spermidine were higher in the ras-transfected cells. Acetylpolyamines were excreted into the medium by the ras-transfected cells, whereas they were retained by NIH 3T3 cells. In addition to acetylpolyamines, some unknown polyamine conjugates occurred in the ras-transfected cells.
Pakala
R
R
Department of Molecular Biology, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
Kreisel
M
M
Bachrach
U
U
D000107:Acetylation; D002471:Cell Transformation, Neoplastic / Q000378:metabolism*; D002478:Cells, Cultured; D011905:Genes, ras; D011073:Polyamines / Q000378:metabolism*; D014162:Transfection
D011073:Polyamines