title: Function of a yeast TATA element-binding protein in a mammalian transcription system.
abstract: Saccharomyces cerevisiae contains a protein which is functionally similar to the mammalian TATA element-binding transcription factor, TFIID. The yeast factor substitutes for TFIID in a mammalian RNA polymerase II in vitro transcription system, forms a stable preinitiation complex on the Adenovirus-2 major late promoter, and binds specifically to the TATA boxes of the viral promoter and the yeast CYC1 promoter. Interestingly, the yeast factor promotes initiation at a distance from the TATA element typical of a mammalian system.
authors.affiliation: Department of Biology, Massachusetts Institute of Technology, Cambridge 02139.
authors.forename: S
authors.initials: S
authors.lastname: Buratowski
authors.forename: S
authors.initials: S
authors.lastname: Hahn
authors.forename: P A
authors.initials: PA
authors.lastname: Sharp
authors.forename: L
authors.initials: L
authors.lastname: Guarente
mesh_terms: D004268:DNA-Binding Proteins / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D006367:HeLa Cells / Q000378:metabolism; D006801:Humans; D010442:Peptide Chain Initiation, Translational; D011401:Promoter Regions, Genetic; D012319:RNA Polymerase II / Q000502:physiology; D012045:Regulatory Sequences, Nucleic Acid; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D013045:Species Specificity; D014157:Transcription Factors / Q000302:isolation & purification / Q000378:metabolism / Q000502:physiology*; D014158:Transcription, Genetic
chemical_list: D004268:DNA-Binding Proteins; D014157:Transcription Factors; D012319:RNA Polymerase II