title: Genetic and molecular characterization of the guaC-nadC-aroP region of Escherichia coli K-12.
abstract: The guaC (GMP reductase), nadC (quinolinate phosphoribosyltransferase), and aroP (aromatic amino acid permease) genes of Escherichia coli K-12 were located in the 2.5-min region of the chromosome (muT-guaC-nadC-aroP-aceE) by a combination of linkage analysis, deletion mapping, restriction analysis, and plasmid subcloning. The guaC locus expressed a product of Mr 37,000 with a clockwise transcriptional polarity, and the GMP reductase activities of guaC+ plasmid-containing strains were amplified 15- to 20-fold.
authors.affiliation: Department of Microbiology, University of Sheffield, United Kingdom.
authors.forename: R E
authors.initials: RE
authors.lastname: Roberts
authors.forename: C I
authors.initials: CI
authors.lastname: Lienhard
authors.forename: C G
authors.initials: CG
authors.lastname: Gaines
authors.forename: J M
authors.initials: JM
authors.lastname: Smith
authors.forename: J R
authors.initials: JR
authors.lastname: Guest
mesh_terms: D026905:Amino Acid Transport Systems; D001435:Bacteriophages; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D050899:GMP Reductase; D005798:Genes, Bacterial; D026901:Membrane Transport Proteins / Q000235:genetics*; D009247:NADH, NADPH Oxidoreductases / Q000235:genetics*; D010430:Pentosyltransferases / Q000235:genetics*; D010641:Phenotype; D010957:Plasmids; D014158:Transcription, Genetic; D014161:Transduction, Genetic; D014169:Transformation, Bacterial; D000098553:Nicotinate-Nucleotide Diphosphorylase (Carboxylating)
chemical_list: D026905:Amino Acid Transport Systems; D026901:Membrane Transport Proteins; D009247:NADH, NADPH Oxidoreductases; D050899:GMP Reductase; D010430:Pentosyltransferases; D000098553:Nicotinate-Nucleotide Diphosphorylase (Carboxylating)