Cloning and sequencing of spinach cDNA clones encoding the 20 kDa PS I polypeptide.
Apart from the 8 kDa subunit, which is of chloroplast origin, most of the small polypeptides of the PS I reaction center from higher plants are encoded in nuclear genes. We describe here the first nucleotide sequence of a nuclear component of this photosystem, the precursor of the 20 kDa protein. The deduced sequence of the large transit peptide (55-60 amino acids) is rich in serine/threonine residues and has a net positive charge, which are classical features of these precursors. The sequence itself is mainly hydrophilic, with no possibility of classical membrane-spanning alpha-helices; it exhibits an interesting stretch of five basic amino acids in close vicinity: Thr-Arg-Leu-Arg-Ser-Lys-Tyr-Lys-Ile-Lys-Tyr.
Lagoutte
B
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Département de Biologie, CEN Saclay, Gif-sur-Yvette, France.
D000595:Amino Acid Sequence; D001483:Base Sequence; D002734:Chlorophyll / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D007887:Fabaceae; D007118:Immunoassay; D045342:Light-Harvesting Protein Complexes; D008969:Molecular Sequence Data; D045322:Photosynthetic Reaction Center Complex Proteins; D010940:Plant Proteins / Q000235:genetics*; D010944:Plants / Q000235:genetics*; D010946:Plants, Medicinal; D011487:Protein Conformation; D011498:Protein Precursors / Q000235:genetics*; D014675:Vegetables
D045342:Light-Harvesting Protein Complexes; D045322:Photosynthetic Reaction Center Complex Proteins; D010940:Plant Proteins; D011498:Protein Precursors; D002734:Chlorophyll; D004247:DNA