title: Restriction endonucleases in Clostridium pasteurianum ATCC 6013 and C. thermohydrosulfuricum DSM 568.
abstract: A small collection of clostridia was surveyed for type II restriction endonucleases. Enzymes were detected in two organisms. Clostridium pasteurianum ATCC 6013 contains an isoschizomer of ThaI (FnuDII) [5'-CGCG-3'] and preliminary evidence suggests that cleavage generates blunt-ended fragments. Clostridium thermohydrosulfuricum DSM 568 contains an isoschizomer of MboI (Sau3A) [5'-GATC-3'] that is inactive on dam methylated substrates. The DNA of this latter organism shows dam methylation.
authors.affiliation: Department of Botany and Microbiology, University College of Wales, Dyfed, UK.
authors.forename: D F
authors.initials: DF
authors.lastname: Richards
authors.forename: P E
authors.initials: PE
authors.lastname: Linnett
authors.forename: J D
authors.initials: JD
authors.lastname: Oultram
authors.forename: M
authors.initials: M
authors.lastname: Young
mesh_terms: D003013:Clostridium / Q000201:enzymology* / Q000235:genetics; D015252:Deoxyribonucleases, Type II Site-Specific / Q000032:analysis*; D004587:Electrophoresis, Agar Gel
chemical_list: D015252:Deoxyribonucleases, Type II Site-Specific