title: Epidermal growth factor stimulates prostacyclin production by cultured human vascular endothelial cells.
abstract: Epidermal growth factor (EGF) stimulated prostacyclin (PGI2) production by cultured human umbilical vein endothelial cells, as measured by radioimmunoassay of its stable metabolite 6-keto-prostaglandin F1 alpha. This effect of EGF was dose-dependent, the lowest stimulatory concentration of EGF was 1.0 ng/ml and 100 ng/ml caused a 2.7 +/- 0.3 (mean +/- SEM) fold increase in the PGI2 synthesis. The stimulation appeared at 3-6 h of incubation and lasted at least 24 h. It was suppressed by EGF antibodies and blocked by protein synthesis inhibitor cycloheximide. Cells preincubated 12 h with EGF released also higher amounts of PGI2 when incubated with thrombin for 5 min. It is concluded that EGF liberated from platelets during aggregation may prevent local thrombogenesis and atherogenesis by stimulating the release of the antiaggregatory, vasodilatory PGI2 from vascular endothelial cells.
authors.affiliation: Children's Hospital, Helsinki, Finland.
authors.forename: A
authors.initials: A
authors.lastname: Ristimäki
authors.forename: O
authors.initials: O
authors.lastname: Ylikorkala
authors.forename: J
authors.initials: J
authors.lastname: Perheentupa
authors.forename: L
authors.initials: L
authors.lastname: Viinikka
mesh_terms: D015121:6-Ketoprostaglandin F1 alpha / Q000378:metabolism; D002478:Cells, Cultured; D003513:Cycloheximide / Q000494:pharmacology; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000378:metabolism; D004815:Epidermal Growth Factor / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D011464:Epoprostenol / Q000096:biosynthesis*; D006801:Humans; D013917:Thrombin / Q000494:pharmacology
chemical_list: D015121:6-Ketoprostaglandin F1 alpha; D004815:Epidermal Growth Factor; D003513:Cycloheximide; D011464:Epoprostenol; D013917:Thrombin