Plasmodium falciparum: cloning by single-erythrocyte micromanipulation and heterogeneity in vitro.
Natural isolates of Plasmodium falciparum represent a genetically heterogeneous population of parasites. To obtain stable strains of the parasites for long term experiments, a rapid and definitive method of cloning was developed using micropipets and a micromanipulator. Homogeneous parasite clones prepared by this technique were characterized and compared with the parent isolates during 4 years of continuous culture. The process of phenotypic dominance and selection of drug resistance which occur in nature was also simulated in vitro by evaluating population dynamics of two cocultured isolates of P. falciparum.
Oduola
A M
AM
Department of Parasitology and Laboratory Practice, School of Public Health, University of North Carolina, Chapel Hill 27514.
Weatherly
N F
NF
Bowdre
J H
JH
Desjardins
R E
RE
D000818:Animals; D000962:Antimalarials / Q000494:pharmacology; D002478:Cells, Cultured; D002999:Clone Cells; D003470:Culture Media; D004351:Drug Resistance; D004912:Erythrocytes / Q000469:parasitology*; D006801:Humans; D008846:Micromanipulation; D010641:Phenotype; D010963:Plasmodium falciparum / Q000166:cytology* / Q000187:drug effects / Q000254:growth & development
D000962:Antimalarials; D003470:Culture Media