Chicken mucin-cross-reactive antigen.
We have previously described a chicken heterophile antigenic determinant (CHAD-1) shared by Mycobacterium smegmatis and chicken tissues. We then demonstrated that CHAD-1 is present on several chicken glycoproteins and that its immunoreactive domains are highly branched asparagine-linked oligosaccharides terminating in N-acetylglucosamine residues. In the present study, we have shown that CHAD-1 is also expressed by mucin purified to homogeneity from a soluble mucus of chicken intestine. Another antigen found on chicken mucin is a chicken mucin-cross-reactive antigen (CMCRA). Antisera to this antigen were produced by immunization of rabbits with an enriched preparation of CHAD-1 isolated from the bursa of Fabricius. These antisera were absorbed with Mycobacterium smegmatis (to block the anti-CHAD-1 antibody) and with chicken serum, and then used for immunoperoxidase staining of chicken tissue sections for CMCRA. The latter antigen was detected in most medullary cells of the bursa, in epithelial cells and Hassal's corpuscles of the thymus, and in mucus-producing cells of the intestine, esophagus, trachea, and bronchi. Using Western immunoblot analysis, we demonstrated that CMCRA is expressed by a number of polypeptides extracted from bursal lymphoid cells. These polypeptides could not be detected in extracts of thymus, spleen, peripheral blood or bone marrow mononuclear cells.
Chechik
B E
BE
Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada.
Fong
M
M
Greer
W
W
Fernandes
B
B
Harvey
P R
PR
D000818:Animals; D015478:Antigens, Heterophile / Q000276:immunology*; D002645:Chickens / Q000276:immunology*; D003429:Cross Reactions; D005260:Female; D005455:Fluorescent Antibody Technique; D007124:Immunoenzyme Techniques; D008297:Male; D009077:Mucins / Q000276:immunology*; D009161:Mycobacterium / Q000276:immunology
D015478:Antigens, Heterophile; D009077:Mucins