Identification and purification of human erythroid progenitor cells by monoclonal antibody to the transferrin receptor (TU 67).
Anti-TU 67 is a murine monoclonal antibody that recognizes the transferrin receptor. With respect to hematopoietic cells TU 67 is expressed by human multipotent colony-forming cells (CFU-Mix), erythroid progenitor cells (BFU-E and CFU-E) and a fraction of granulocyte/monocyte colony forming cells, but is not expressed by mature hematopoietic cells including erythrocytes, platelets, lymphocytes, and peripheral blood myeloid cells. The TU 67-positive fraction of normal bone marrow, separated by fluorescence-activated cell sorting (FACS) or immune rosettes, contained 87% of the erythroid progenitor cells. Erythroid progenitor cells were enriched up to 50-fold by using a combination of monoclonal antibodies to deplete mature hematopoietic cells, followed by positive selection of BFU-E and CFU-E by TU 67 antibody.
Herrmann
F
F
Abteilung für Hämatologie, I. Medizinische Klinik, Johannes Gutenberg-Universität, Mainz, Federal Republic of Germany.
Griffin
J D
JD
Sabbath
K D
KD
Oster
W
W
Wernet
P
P
Mertelsmann
R
R
D000911:Antibodies, Monoclonal; D002469:Cell Separation / Q000379:methods; D004900:Erythroblasts / Q000166:cytology*; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D011990:Receptors, Transferrin / Q000276:immunology*; D012397:Rosette Formation
D000911:Antibodies, Monoclonal; D011990:Receptors, Transferrin