Hafnia alvei lipopolysaccharides: isolation, sugar composition and SDS-PAGE analysis.
Lipopolysaccharides (LPS) of 33 strains of Hafnia alvei were isolated and purified. LPS content of the dry bacterial mass ranged from 1.2 to 4.5%. All examined lipopolysaccharides contained glucose, glucosamine, heptose, 3-deoxy-octulosonic acid and often galactose. Rhamnose, mannose, galactosamine, mannosamine and unidentified amino sugars were found in some H. alvei strains. Sialic acid was present in LPS of one strain. D-3-Hydroxybutyryl groups also were identified in lipopolysaccharides of 5 strains of this genus. SDS-PAGE of the lipopolysaccharides was presented in the paper. According to these results two core types exist in H. alvei.
Romanowska
A
A
Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Wrocław, Poland.
Katzenellenbogen
E
E
Kułakowska
M
M
Gamian
A
A
Witkowska
D
D
Mulczyk
M
M
Romanowska
E
E
D002241:Carbohydrates / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D004755:Enterobacteriaceae / Q000032:analysis* / Q000145:classification; D008070:Lipopolysaccharides / Q000302:isolation & purification*; D012967:Sodium Dodecyl Sulfate; D013045:Species Specificity
D002241:Carbohydrates; D008070:Lipopolysaccharides; D012967:Sodium Dodecyl Sulfate