title: Local administration of cells containing an inserted IL-2 gene and producing IL-2 inhibits growth of human tumours in nu/nu mice.
abstract: We have prepared a retroviral expression construct, pPS-IL-2, in which human IL-2 cDNA has been inserted into the polylinker region, and have used the retroviral vector to introduce the functional IL-2 gene into a fibroblast cell line, RAT-1. Peritumoral administration of IL-2-producing RAT-1 cells into congenitally athymic (nu/nu) mice carrying subcutaneous transplants of human carcinoma cells inhibited the growth of the human tumour xenografts.
authors.affiliation: Institute of Molecular Genetics, Czechoslovak Academy of Sciences, Prague.
authors.forename: J
authors.initials: J
authors.lastname: Bubenik
authors.forename: N N
authors.initials: NN
authors.lastname: Voitenok
authors.forename: J
authors.initials: J
authors.lastname: Kieler
authors.forename: V S
authors.initials: VS
authors.lastname: Prassolov
authors.forename: P M
authors.initials: PM
authors.lastname: Chumakov
authors.forename: D
authors.initials: D
authors.lastname: Bubenikova
authors.forename: J
authors.initials: J
authors.lastname: Simova
authors.forename: T
authors.initials: T
authors.lastname: Jandlova
mesh_terms: D000818:Animals; D005347:Fibroblasts / Q000276:immunology / Q000637:transplantation; D005818:Genetic Engineering; D005822:Genetic Vectors; D006367:HeLa Cells / Q000276:immunology / Q000473:pathology; D006801:Humans; D007376:Interleukin-2 / Q000096:biosynthesis / Q000235:genetics*; D007694:Killer Cells, Natural / Q000276:immunology; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000235:genetics / Q000276:immunology / Q000628:therapy*; D012190:Retroviridae / Q000235:genetics; D014170:Transformation, Genetic
chemical_list: D007376:Interleukin-2