The chemical characterization of the radioactive products derived from [[3H]PheB1]insulin in the circulation of the rat.
1. Gel filtration of rat plasma taken 1 h after subcutaneous injection of [[3H]PheB1]insulin gives three peaks of radioactivity. 2. The material in these peaks was characterized by electrophoresis and chromatography. 3. We conclude that [[3H]PheB1]insulin is rapidly degraded to free tritiated phenylalanine. The phenylalanine is subsequently used for synthesis of plasma proteins de novo.
Davies
J G
JG
Département de Biochimie Médicale, Centre Médical Universitaire, Genève, Switzerland.
Offord
R E
RE
D000595:Amino Acid Sequence; D000818:Animals; D002850:Chromatography, Gel; D004588:Electrophoresis, Cellulose Acetate; D005561:Formates; D007328:Insulin / Q000031:analogs & derivatives* / Q000097:blood*; D010084:Oxidation-Reduction; D010446:Peptide Fragments / Q000032:analysis; D010649:Phenylalanine / Q000378:metabolism; D051381:Rats; D014316:Tritium
D005561:Formates; D007328:Insulin; D010446:Peptide Fragments; C054929:insulin, Phe(B1)-; D014316:Tritium; C008817:peroxyformic acid; D010649:Phenylalanine