title: The alternative respiratory pathway of the yeast Candida parapsilosis: oxidation of exogenous NAD(P)H.
abstract: The yeast Candida parapsilosis possesses two routes of electron transfer from exogenous NAD(P)H to oxygen. Electrons are transferred either to the classical cytochrome pathway at the level of ubiquinone through an NAD(P)H dehydrogenase, or to an alternative pathway at the level of cytochrome c through another NAD(P)H dehydrogenase which is insensitive to antimycin A. Analyses of mitoplasts obtained by digitonin/osmotic shock treatment of mitochondria purified on a sucrose gradient indicated that the NADH and NADPH dehydrogenases serving the alternative route were located on the mitochondrial inner membrane. The dehydrogenases could be differentiated by their pH optima and their sensitivity to amytal, butanedione and mersalyl. No transhydrogenase activity occurred between the dehydrogenases, although NADH oxidation was inhibited by NADP+ and butanedione. Studies of the effect of NADP+ on NADH oxidation showed that the NADH:ubiquinone oxidoreductase had Michaelis-Menten kinetics and was inhibited by NADP+, whereas the alternative NADH dehydrogenase had allosteric properties (NADH is a negative effector and is displaced from its regulatory site by NAD+ or NADP+).
authors.affiliation: Institut de Biochimie Cellulaire et de Neurochimie du CNRS, Bordeaux, France.
authors.forename: N M
authors.initials: NM
authors.lastname: Camougrand
authors.forename: A
authors.initials: A
authors.lastname: Cheyrou
authors.forename: M F
authors.initials: MF
authors.lastname: Henry
authors.forename: M G
authors.initials: MG
authors.lastname: Guérin
mesh_terms: D002175:Candida / Q000378:metabolism*; D004579:Electron Transport; D008928:Mitochondria / Q000201:enzymology; D009249:NADP / Q000378:metabolism*; D009250:NADP Transhydrogenases / Q000502:physiology; D009252:NADPH Dehydrogenase / Q000378:metabolism; D010084:Oxidation-Reduction
chemical_list: D009249:NADP; D009250:NADP Transhydrogenases; D009252:NADPH Dehydrogenase