title: Yeast vacuoles fragment when microtubules are disrupted.
abstract: To identify whether microtubules are involved in the maintenance of vacuolar morphology, we treated Saccharomyces cerevisiae with nocodazole and methyl benzimidazole-2-yl-carbamate, drugs which inhibit the polymerization of microtubules. Treated cells arrest with a single large bud in the G2/prophase portion of the cell cycle. Labeling the vacuole with either quinacrine or FITC-dextran revealed vacuole fragmentation that was not found in untreated cells or in cells arrested in G2 by unrelated means. A drug-resistant mutant in beta tubulin does not show vacuolar fragmentation when treated with drug. We propose that microtubules are involved in the regulation of vacuole morphology.
authors.affiliation: Molecular Biology Institute, University of California, Los Angeles 90024.
authors.forename: B A
authors.initials: BA
authors.lastname: Guthrie
authors.forename: W
authors.initials: W
authors.lastname: Wickner
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology; D001562:Benzimidazoles / Q000494:pharmacology; D005455:Fluorescent Antibody Technique; D007399:Interphase; D008870:Microtubules / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure*; D015739:Nocodazole; D009940:Organoids / Q000648:ultrastructure*; D011418:Prophase; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure*; D014617:Vacuoles / Q000187:drug effects / Q000648:ultrastructure*
chemical_list: D000935:Antifungal Agents; D001562:Benzimidazoles; D015739:Nocodazole