Comparison between enzyme-linked immunosorbent and I.F. assays in the serological diagnosis of HIV infections.
A rapid, sensitive indirect immunofluorescence assay based on the use of acetone-fixed virus producing MOLT T4/LAV cells was adapted for the detection of anti-HIV antibodies. At the same time, 1486 serum samples, collected by the AIDS Surveillance and Study Centre of Modena, were tested by ELISA and IFA: the percentage of agreement of both assays was of 99.66%. Such datum suggested that IFA could be used as a serological assay for screening and confirmatory purposes.
Borghi
V
V
Department of Infectious Diseases, University of Modena, Italy.
De Palma
M
M
Vecchi
M
M
Pellegrino
F
F
Mongiardo
N
N
Cocchi
I
I
De Rienzo
B
B
Squadrini
F
F
D000163:Acquired Immunodeficiency Syndrome / Q000175:diagnosis*; D000914:Antibodies, Viral / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D005188:False Negative Reactions; D005189:False Positive Reactions; D005455:Fluorescent Antibody Technique; D006678:HIV / Q000276:immunology*; D015483:HIV Antibodies; D006801:Humans
D000914:Antibodies, Viral; D015483:HIV Antibodies