Production of antibodies and development of an immunoassay for the anticoagulant, diphacinone.
Diphacinone, a commonly used anticoagulant rodenticide, was coupled to protein via an (O-carboxymethyl) oxime bridge. Immunization in rabbits produced antibodies with good ability to recognize the hapten as demonstrated by indirect EIA and affinity column adsorption. A competitive EIA was developed which clearly measured 10 micrograms/L diphacinone concentrations showing the sensitivity of the assay. Cross-reactivity study with chlorophacinone showed that the antisera possessed a high degree of diphacinone specificity.
Mount
M E
ME
Department of Pharmacology and Toxicology, School of Veterinary Medicine, University of California, Davis 95616.
Kurth
M J
MJ
Jackson
D Y
DY
D000818:Animals; D000915:Antibody Affinity; D000917:Antibody Formation; D000925:Anticoagulants / Q000032:analysis / Q000276:immunology*; D001667:Binding, Competitive; D003429:Cross Reactions; D006241:Haptens / Q000276:immunology; D007124:Immunoenzyme Techniques; D010630:Phenindione / Q000031:analogs & derivatives* / Q000032:analysis / Q000276:immunology; D011817:Rabbits
D000925:Anticoagulants; D006241:Haptens; C100262:diphenadione; D010630:Phenindione