The influence of tetraphenylborates (hydrophobic anions) on yeast cell electro-rotation.
The action of a series of tetraphenylborate ion (TPB) derivatives on yeast cells was studied by electro-rotation of the pre-treated cells. TPB derivatives in which all four phenyl groups were substituted with fluorine, chlorine or trifluoromethyl were much more toxic than the unsubstituted compound, the effect increasing dramatically with increasing size of substituents. These observations suggest that the toxicity of these hydrophobic ions is determined mainly by their size and possibly also by the chemical inductivity of their substituent groups. The order of the toxicities of these ions was in fair agreement with literature values for their translocation rates across artificial bilayers. Incubation times of 3 h were used as standard, longer incubations (up to 48 h) showed that the number of cells affected by low doses of TPB increased with the logarithm of time after the first hour of incubation. Although measurements of the percentage of cells showing co-field rotation showed that controls were not adversely affected by incubations as long as 9 h, rotation spectra showed that some cells suffer loss of internal conductivity during extended incubations. Decrease of the pH of the incubation medium, or inclusion of high concentrations of NaCl or KCl, potentiated the effects of these hydrophobic ions. The toxicity developed slowly, and the sensitivity of the assay was only very weakly dependent on the cell suspension density.
Arnold
W M
WM
Lehrstuhl für Biotechnologie der Universität Würzburg, F.R.G.
Zimmermann
U
U
Heiden
W
W
Ahlers
J
J
D001896:Boron Compounds / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D004574:Electromagnetic Fields; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D009994:Osmolar Concentration; D012399:Rotation; D012441:Saccharomyces cerevisiae / Q000187:drug effects*; D012492:Salts; D012995:Solubility; D013329:Structure-Activity Relationship; D013775:Tetraphenylborate / Q000008:administration & dosage / Q000633:toxicity*
D001896:Boron Compounds; D012492:Salts; D013775:Tetraphenylborate