Laminin, fibronectin and type IV collagen in BM-like material from cultured arterial smooth muscle cells.
1. The intra- and extracellular distribution of fibronectin and laminin was studied by immunofluorescence in cultures of rabbit and human arterial smooth muscle cells. 2. Basement membrane (BM)-like material was isolated from the cell layer of arterial smooth muscle cells cultures and analysed by sodium dodecyl sulphate gel electrophoresis (SDS-PAGE) and immunoblotting. The major 220-240 kD component of arterial BM-like material was identified as fibronectin. Also a 200 kD fibronectin band was observed. 3. The 200 kD subunit of laminin was contained in isolated BM-like material, but no slower migrating laminin chains were detected. 4. Collagens were prepared from pepsinized BM-like material. The band pattern as resolved by SDS-PAGE and silver staining suggested that type IV collagen is the major collagen of arterial BM-like material.
Heickendorff
L
L
Department of Clinical Chemistry, Aarhus Kommunehospital, University of Aarhus, Denmark.
D000818:Animals; D002478:Cells, Cultured; D003094:Collagen / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D005353:Fibronectins / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D007163:Immunosorbent Techniques; D007797:Laminin / Q000032:analysis*; D008970:Molecular Weight; D009131:Muscle, Smooth, Vascular / Q000032:analysis*; D011817:Rabbits
D005353:Fibronectins; D007797:Laminin; D003094:Collagen