title: [Determination of ochratoxin A in human milk].
abstract: A method for the determination of ochratoxin A in milk is described. The milk is homogenized in a buffer solution at pH 1.6 to release ochratoxin A from its bond to proteins. Ochratoxin A is extracted with chloroform and the extract cleaned up using a base clean-up step. Analysis is performed by high-pressure liquid chromatography, using a reversed-phase column and fluorescence detection. The detection limit of the method is 0.1 ng/ml and the average recovery rate, tested in the range between 0.5 and 10.0 ng/ml, was found to be 83.1%. Chemical ionization mass spectrometry (direct exposure probe) and an enzyme immunoassay were used as confirmatory tests. Using this method, trace amounts of ochratoxin A were found in 4 of 36 randomly collected human milk samples.
authors.affiliation: Institut für Medizinische Mikrobiologie, Infektions- und Seuchenmedizin, Ludwig-Maximilians-Universität München, Bundesrepublik Deutschland.
authors.forename: M
authors.initials: M
authors.lastname: Gareis
authors.forename: E
authors.initials: E
authors.lastname: Märtlbauer
authors.forename: J
authors.initials: J
authors.lastname: Bauer
authors.forename: B
authors.initials: B
authors.lastname: Gedek
mesh_terms: D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D013058:Mass Spectrometry; D008895:Milk, Human / Q000032:analysis*; D009793:Ochratoxins / Q000032:analysis*
chemical_list: D009793:Ochratoxins; C025589:ochratoxin A
vernacular_title: Bestimmung von Ochratoxin A in Muttermilch.