title: Integration host factor of Escherichia coli regulates early- and repressor transcription of bacteriophage Mu by two different mechanisms.
abstract: Integration host factor (IHF) of E. coli positively regulates both early and repressor transcription of bacteriophage Mu. In this paper we show that although binding of IHF to the same binding site is responsible for both types of transcription regulation, the mechanisms by which these regulations occur are different: Activation of transcription from the early promoter (Pe) requires a helix-dependent orientation of IHF- and RNA polymerase binding sites on the DNA helix with a limited distance between both sites. Activation of repressor transcription shows no helix dependency between promoter and IHF binding site and the distance between both sites can be enlarged at least by 100 base pairs without affecting the positive control. A possible mechanism for both types of transcription stimulation will be discussed.
authors.affiliation: Department of Molecular Genetics, State University of Leiden, The Netherlands.
authors.forename: P A
authors.initials: PA
authors.lastname: van Rijn
authors.forename: N
authors.initials: N
authors.lastname: Goosen
authors.forename: P
authors.initials: P
authors.lastname: van de Putte
mesh_terms: D001426:Bacterial Proteins / Q000502:physiology*; D001483:Base Sequence; D002872:Chromosome Deletion; D003090:Coliphages / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D005786:Gene Expression Regulation; D005814:Genes, Viral; D034982:Integration Host Factors; D008969:Molecular Sequence Data; D010957:Plasmids; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D004279:DNA, Viral; D004268:DNA-Binding Proteins; D034982:Integration Host Factors