Detection and purification of a recombinant human B lymphotropic virus (HHV-6) in the baculovirus expression system by limiting dilution and DNA dot-blot hybridization.
A recombinant virus Ac373-HB was detected and purified from the transfection mixture of wild type and recombinant virus in the baculovirus expression system using a combination of limiting dilution and DNA dot-blot hybridization. This method allows for a quick and convenient way of detection and purification of recombinant virus without the need to use a plaque purification step. It is generally applicable to other expression systems besides the baculovirus system described here.
Fung
M C
MC
Department of Virology and Epidemiology, Baylor College of Medicine, Houston, Texas 77030.
Chiu
K Y
KY
Weber
T
T
Chang
T W
TW
Chang
N T
NT
D001402:B-Lymphocytes / Q000382:microbiology*; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D005822:Genetic Vectors; D006564:Herpesviridae / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D007304:Insect Viruses / Q000235:genetics; D008828:Microbiological Techniques; D009693:Nucleic Acid Hybridization; D011995:Recombination, Genetic
D004279:DNA, Viral