Further biochemical characterization of human basophil-like cell-promoting activity and its distinction from human interleukin-3-like activity.
Supernatants from lectin-stimulated human peripheral blood mononuclear cells contained two activities; basophil-like cell-promoting activity (BaPA) and a growth factor for murine interleukin-3-dependent cell lines (IL-3-like activity). BaPA had a MW of 24,000 and a pI of 6.0-7.5, while human IL-3-like activity had a MW of 18,000 and a pI of 5.0-5.9. Beside the biochemical differences between the two activities, supernatants from a T cell line were found that contained BaPA and GM-CSF but no IL-3-like activity. These data suggest that BaPA and IL-3-like activity are two different molecular entities.
Tadokoro
K
K
Institute of Clinical Immunology, University of Bern, Switzerland.
Stadler
B M
BM
de Weck
A L
AL
D000645:Ammonium Sulfate; D000818:Animals; D001491:Basophils / Q000166:cytology*; D001798:Blood Proteins; D002460:Cell Line; D002474:Cell-Free System; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D003115:Colony-Stimulating Factors / Q000502:physiology; D003208:Concanavalin A; D003470:Culture Media / Q000032:analysis; D005590:Fractional Precipitation; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D006133:Growth Substances / Q000097:blood / Q000302:isolation & purification / Q000502:physiology*; D006358:Hot Temperature; D006801:Humans; D007377:Interleukin-3 / Q000502:physiology*; D007525:Isoelectric Focusing; D051379:Mice; D011506:Proteins / Q000302:isolation & purification / Q000502:physiology*
D001798:Blood Proteins; D003115:Colony-Stimulating Factors; D003470:Culture Media; D006133:Growth Substances; D007377:Interleukin-3; D011506:Proteins; D003208:Concanavalin A; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D000645:Ammonium Sulfate