Detection of H-2K mRNA in mouse 8-cell embryo by cDNA cloning.
Mouse MHC class I gene expression in 8-cell embryo was examined by cDNA cloning. We constructed a cDNA library from 8-cell embryos of ICR mice and isolated a class I cDNA from 3.0 x 10(5) phage clones of the library. Sequencing analysis of this clone revealed it to include the cDNA fragment extending from the exon 6 of the cytoplasmic portion to 3' untranslated region 1 of H-2K gene. Qa, Tla or other embryonic class I cDNA have not been isolated in the library.
Nagata
T
T
Department of Microbial Genetics, Osaka University.
Nozaki
M
M
Morita
T
T
Matsushiro
A
A
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000096:biosynthesis; D004622:Embryo, Mammalian / Q000032:analysis*; D015723:Gene Library; D006183:H-2 Antigens / Q000235:genetics*; D051379:Mice; D008813:Mice, Inbred ICR; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis*
D006183:H-2 Antigens; C032876:H-2Kb protein, mouse; D012333:RNA, Messenger; D004247:DNA