[The role of in vivo interaction of proteins SSB with DNA polymerase II].
In order to elucidate the role of in vivo interaction of single-strand DNA binding protein with DNA polymerase II isogenic strains of Escherichia coli were constructed combining the ssb+, ssb-1 alleles with DNA polymerases I or II mutations; their radiosensitivity and a level of UV-induced DNA degradation were studied. Received findings suggest a functional antagonism of SSB-proteins depending on intracellular conditions (from the balance of DNA and protein synthesis). The SSB-proteins provide the stability of the genome or, vice versa, perturb the stability of the genome, by degrading of DNA macromolecules.
Fradkin
G E
GE
Rabinkova
E V
EV
Torosian
M V
MV
Aĭzenberg
O A
OA
Naumova
L A
LA
D004247:DNA / Q000528:radiation effects; D004257:DNA Polymerase II / Q000378:metabolism*; D004261:DNA Replication; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics; D008957:Models, Genetic; D009154:Mutation; D014466:Ultraviolet Rays
D004268:DNA-Binding Proteins; D004247:DNA; D004257:DNA Polymerase II
Rol' vzaimodeĭstviia in vivo belkov SSB s DNK-polimerazoĭ II.