Cloning of a phosphinothricin N-acetyltransferase gene from Streptomyces viridochromogenes Tü494 and its expression in Streptomyces lividans and Escherichia coli.
Phosphinothricin-tripeptide (Ptt), also known as bialaphos, contains phosphinothricin (Pt), a potent inhibitor of glutamine synthetase. A 4.0-kb Bam HI fragment coding for Ptt resistance was cloned in Streptomyces lividans TK23. The fragment was isolated from a Ptt-resistant mutant of Streptomyces viridochromogenes Tü494. Subcloning experiments revealed that Ptt resistance can be assigned to a 0.8-kb Bg/II fragment. This fragment was shown to include the Ptt-resistance promoter. Subcloning this fragment downstream from the lacZ promoter conferred Ptt resistance to Escherichia coli JM83 in one of the two possible orientations. Biochemical investigations revealed that the Bg/II fragment codes for a Pt N-acetyltransferase.
Strauch
E
E
Lehrstuhl für Genetik, Fakultät für Biologie, Universität Bielefeld, F.R.G.
Wohlleben
W
W
Pühler
A
A
D000123:Acetyltransferases / Q000096:biosynthesis / Q000235:genetics*; D000613:Aminobutyrates / Q000494:pharmacology; D001426:Bacterial Proteins / Q000096:biosynthesis*; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000187:drug effects / Q000378:metabolism; D005798:Genes, Bacterial; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000096:biosynthesis; D013302:Streptomyces / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism
D000613:Aminobutyrates; D001426:Bacterial Proteins; D011994:Recombinant Proteins; C003121:phosphinothricin; D000123:Acetyltransferases; C056243:phosphinothricin N-acetyltransferase