Distribution of actin filaments in human malignant keratinocytes.
Distribution of actin filaments in human malignant keratinocytes was examined by immunofluorescence staining. The primary cultures were obtained from a squamous cell carcinoma, a basal cell carcinoma, and Bowen's disease. Rhodamine-phalloidin staining revealed that actin filaments were occasionally organized to form stress fibers, many short bundles with a ripple appearance, and regular arrays of actin patches. Some of these structures appeared in untransformed keratinocytes as a result of a brief exposure to a tumor promotor, TPA. These findings suggest that regulation of actin functions is involved in neoplastic processes from the very early stages and that alteration is persistent in neoplastic cells.
Kitano
Y
Y
Department of Dermatology, Osaka University School of Medicine, Japan.
D000199:Actins / Q000032:analysis*; D000368:Aged; D001913:Bowen's Disease / Q000032:analysis / Q000648:ultrastructure*; D002280:Basal Cell Carcinoma / Q000032:analysis / Q000648:ultrastructure*; D002294:Carcinoma, Squamous Cell / Q000032:analysis / Q000648:ultrastructure*; D005260:Female; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes; D006801:Humans; D008297:Male; D008875:Middle Aged; D010590:Phalloidine / Q000031:analogs & derivatives; D012235:Rhodamines; D012878:Skin Neoplasms / Q000032:analysis / Q000648:ultrastructure*; D014407:Tumor Cells, Cultured
D000199:Actins; D005456:Fluorescent Dyes; D012235:Rhodamines; D010590:Phalloidine; C041085:tetramethylrhodaminylphalloidine