title: Primary structure of the chicken c-mil protein:identification of domains shared with or absent from the retroviral v-mil protein.
abstract: The complete primary structure of the protein product of the proto-oncogene c-mil was deduced from the nucleotide sequence of chicken c-mil cDNA clones. The c-mil protein contains 647 amino acid residues and has a calculated molecular weight of 73,132. Based on sequence comparisons with proteins of known or presumed biochemical function, two domains were recognized on the c-mil protein. In the carboxyl-terminal half of the protein, a 250-amino acid segment displays significant homology to the protein kinase domains of the src oncogene protein or of protein kinase C. In the amino-terminal half, a cysteine-rich segment (Cys-X2-Cys-X9-Cys-X2-Cys-X7-Cys-X7-Cys) of the c-mil protein shares significant homology with two similar repetitive domains of protein kinase C. Of the two structural and presumably functional domains of the c-mil protein, only the kinase domain is contained within the carboxyl-terminal 379-amino acid polypeptide encoded by the transduced v-mil allele of avian oncogenic retrovirus MH2. Hence, truncation of the 5' coding region in the course of the transduction and the resulting lack of the authentic amino-terminal domain in the protein product of the transduced allele may be a critical event in changing mil function from physiologic to oncogenic.
authors.affiliation: Zentrum für Molekulare Biologie der Universität Heidelberg (ZMBH), FRG.
authors.forename: M
authors.initials: M
authors.lastname: Koenen
authors.forename: A E
authors.initials: AE
authors.lastname: Sippel
authors.forename: C
authors.initials: C
authors.lastname: Trachmann
authors.forename: K
authors.initials: K
authors.lastname: Bister
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002645:Chickens; D003001:Cloning, Molecular; D008969:Molecular Sequence Data; D011487:Protein Conformation; D011493:Protein Kinase C; D011518:Proto-Oncogene Proteins
chemical_list: D011518:Proto-Oncogene Proteins; D011493:Protein Kinase C