[The nitroblue tetrazolium reduction test in evaluating the function of the circulating leukocytes in mice with experimental malaria].
NBT-test for circulating neutrophils and monocytes in the blood of mice inoculated with Plasmodium berghei, strain N or LNK-65, have been performed. Within the first 24 h of the infection, before the onset of the recordable parasitemia or in the course of the subsequent six days (depending of the strain used for inoculation) a 50-100% reduction in NBT-positive cells was observed. This demonstrates the ability of malaria parasite to suppress the oxygen-dependent enzyme system in circulating phagocytes, neutrophils and monocytes of the host blood. The results of NBT-test could be utilized for the investigation of immunological disorders and also for the differential diagnosis of malarial infection.
Gilpin-Dzhekson
E
E
Shadrin
B P
BP
D000818:Animals; D007962:Leukocytes / Q000502:physiology*; D008288:Malaria / Q000097:blood* / Q000469:parasitology; D008297:Male; D051379:Mice; D009000:Monocytes / Q000502:physiology; D009504:Neutrophils / Q000502:physiology; D009580:Nitroblue Tetrazolium / Q000097:blood; D010084:Oxidation-Reduction; D010962:Plasmodium berghei; D013778:Tetrazolium Salts; D013997:Time Factors
D013778:Tetrazolium Salts; D009580:Nitroblue Tetrazolium
Test vosstanovleniia nitrosinego tetrazoliia v otsenke funktsional'nogo sostoianiia tsirkuliruiushchikh leĭkotsitov mysheĭ pri éksperimental'noĭ maliarii.