Enzyme immunoassay of immunoreactive trypsin in serum and blood spots.
An enzyme immunoassay method for the assay of serum immunoreactive trypsin (IRT) is described. The method is a two site binding assay carried out on microtitre plates as the solid phase. Wells were coated with affinity purified anti-human trypsin and biotinylated anti-trypsin and avidin-beta-galactosidase were used as the second antibody and detection system respectively. The assay was sensitive enough to determine IRT concentrations in either serum or dried blood spots. A good correlation was obtained when the method was compared with the Hoechst radioimmunoassay method.
Deam
S M
SM
Department of Medical Microbiology, University of Wales College of Medicine, Cardiff, U. K.
Ryley
H C
HC
D001801:Blood Stains; D006801:Humans; D007124:Immunoenzyme Techniques; D011863:Radioimmunoassay; D014357:Trypsin / Q000097:blood*
D014357:Trypsin