Regulation of 70-kilodalton heat-shock-like messenger ribonucleic acid in vitro and in vivo by prolactin.
A cDNA clone, pNb29, was isolated from a library made from mRNA of Nb2 rat lymphoma cells stimulated by PRL. The nucleotide sequence of pNb29 was found to be identical to the 70-kilodalton heat-shock-like (hsp-70-like) mRNA. The levels of this mRNA increased 8 +/- 3-fold after PRL stimulation of arrested Nb2 cells, and is expressed in different amounts in the normal rat tissues analyzed. To determine whether hsp-70-like mRNA was PRL responsive in vivo, ovine PRL was administered to hypophysectomized rats. A 5 +/- 2-fold increase in hepatic hsp-70-like mRNA was observed 9 h after injection. Thus PRL appears to regulate a heat-shock-like mRNA both in vitro and in vivo. This novel finding extends the already wide range of biological effects ascribed to PRL.
deToledo
S M
SM
Department of Physiology, Faculty of Medicine, University of Manitoba, Winnipeg, Canada.
Murphy
L J
LJ
Hatton
T H
TH
Friesen
H G
HG
D000818:Animals; D002460:Cell Line; D005260:Female; D015723:Gene Library; D006360:Heat-Shock Proteins / Q000235:genetics*; D007016:Hypophysectomy; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008223:Lymphoma; D008297:Male; D008970:Molecular Weight; D011388:Prolactin / Q000494:pharmacology*; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics*; D051381:Rats
D006360:Heat-Shock Proteins; D012333:RNA, Messenger; D011388:Prolactin