The influence of IL-1 treatment on the reconstitution of the hemopoietic and immune systems after sublethal radiation.
The influence of IL-1 administration on the recovery of the hemopoietic and immune systems from sublethal irradiation was assessed. Mice were irradiated (750 R) and injected twice daily with purified recombinant derived IL-1 beta (200 ng/injection). At various times after irradiation, the functional capacity of the hemopoietic and immune systems was determined. It was found that IL-1 therapy resulted in a significantly greater number of granulocyte-macrophage-CSF responsive colony-forming cells in the bone marrow of the irradiated mice on days 5 and 11 postirradiation but not at later times. In addition the radiation induced neutropenia recovered quicker in the IL-1-treated mice with significantly greater numbers of peripheral blood granulocytes being seen on days 15 and 20 after irradiation. The influence of IL-1 therapy on the recovery of the immune system was also assessed. Of note was the observation that mice receiving IL-1 therapy had chronically hypoplastic thymi. Although thymic cellularity increased with time after irradiation in the control mice, there was no such increase in the IL-1-treated mice. Similarly, the number of pre-B cells in the marrow of these mice was also diminished. Thus, in the IL-1-treated mice the regeneration of the peripheral immune function was retarded, characterized by a general lymphopenia and decreased splenic responses to mitogenic stimuli.
Morrissey
P
P
Immunex Corporation, Seattle, WA 98101.
Charrier
K
K
Bressler
L
L
Alpert
A
A
D000818:Animals; D001853:Bone Marrow / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D005260:Female; D006410:Hematopoiesis / Q000187:drug effects* / Q000528:radiation effects; D007111:Immunity, Cellular / Q000187:drug effects* / Q000528:radiation effects; D007375:Interleukin-1 / Q000627:therapeutic use*; D007958:Leukocyte Count / Q000187:drug effects / Q000528:radiation effects; D008213:Lymphocyte Activation / Q000187:drug effects / Q000528:radiation effects; D051379:Mice; D008810:Mice, Inbred C57BL; D012038:Regeneration / Q000187:drug effects; D013154:Spleen / Q000166:cytology; D014916:Whole-Body Irradiation / Q000009:adverse effects*
D007375:Interleukin-1