The ionic strength dependence of the cooperativity factor for DNA melting.
The melting temperature for the d(AT)24.d(AT)24 stretch, located inside the DNA helix and terminally, have been determined in a wide range of ionic strength values (0.01 - 1 M Na+). The cooperativity factor was calculated from the shifts in the melting temperature of the stretch due to its different boundary conditions. With the sodium concentration decreasing from 1 M to 0.01 M the cooperativity factor dropped by three orders of magnitude, its change being less marked at high than at low ionic strength.
Kozyavkin
S A
SA
Institute of Molecular Genetics USSR Academy of Sciences, Moscow.
Mirkin
S M
SM
Amirikyan
B R
BR
D004247:DNA / Q000187:drug effects / Q000648:ultrastructure*; D009691:Nucleic Acid Denaturation / Q000187:drug effects; D009994:Osmolar Concentration; D010957:Plasmids; D012965:Sodium Chloride / Q000494:pharmacology; D013696:Temperature
D012965:Sodium Chloride; D004247:DNA