Mechanism of autonomous control of the Escherichia coli F plasmid: purification and characterization of the repE gene product.
E protein, the 29 Kd repE gene product, is essential for the replication of the Escherichia coli F plasmid. The repE gene has been cloned and expressed in an inducible ATG-fusion vector, and the protein product has been purified to homogeneity. The purified E protein is present as a dimer in solution and binds specifically to both the 19-bp direct repeats (incB) found within the mini-F origin of replication ori2 and the repE operator DNA. Examination of the amino acid sequence of E protein revealed a consensus sequence for DNA binding.
Masson
L
L
Department of Biology, University of California, Los Angeles 90024.
Ray
D S
DS
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics* / Q000302:isolation & purification; D004261:DNA Replication; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000302:isolation & purification; D004926:Escherichia coli / Q000235:genetics*; D005144:F Factor; D007700:Kinetics; D009875:Operator Regions, Genetic
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins