title: Vitamin D-dependent calcium binding protein in rat uterus: differential effects of estrogen, tamoxifen, progesterone, and pregnancy on accumulation and cellular localization.
abstract: The present studies were undertaken to characterize the expression of calcium binding protein (CaBP or calbindin-D9k) in uterine tissues. Using immunohistochemical techniques, calbindin-D9k was localized to the uterine (luminal) epithelium of pregnant rats, but not present in the uterine epithelium of nonpregnant rats. Calbindin was found also in the uterine smooth muscle and endometrial stromal cells of pregnant animals. These latter localizations were reproduced in uteri of 21-day-old nonpregnant rats by administration of tamoxifen or physiological doses of estrogens. Estrogen and tamoxifen produced half-maximal increases of uterine calbindin at daily doses of 0.1 and 10 micrograms, respectively, and maximal responses at 0.3 and 40 micrograms/day. Testosterone and progesterone, at doses which increased the growth of the uterus, did not induce calbindin-D, and both hormones blocked estradiol's effect on uterine calbindin-D appearance. The epithelial localization of calbindin in pregnant uteri was not reproduced in nonpregnant animals by either estradiol (3 micrograms/day) or progesterone (1 mg/day). The localization of calbindin in uterine epithelium during pregnancy appears to be dependent upon an as yet unknown factor. In view of the large surface area of the luminal epithelium in pregnant animals, and the pregnancy-related expression of calbindin in these cells, we propose that uterine epithelium plays an important role in transport of calcium during pregnancy.
authors.affiliation: Department of Pathology, University of Virginia Medical School, Charlottesville 22908.
authors.forename: M E
authors.initials: ME
authors.lastname: Bruns
authors.forename: J G
authors.initials: JG
authors.lastname: Overpeck
authors.forename: G C
authors.initials: GC
authors.lastname: Smith
authors.forename: G N
authors.initials: GN
authors.lastname: Hirsch
authors.forename: S E
authors.initials: SE
authors.lastname: Mills
authors.forename: D E
authors.initials: DE
authors.lastname: Bruns
mesh_terms: D000818:Animals; D004848:Epithelium / Q000378:metabolism; D004958:Estradiol / Q000494:pharmacology; D004964:Estriol / Q000494:pharmacology; D004967:Estrogens / Q000494:pharmacology*; D005260:Female; D006651:Histocytochemistry; D007124:Immunoenzyme Techniques; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000378:metabolism*; D011374:Progesterone / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D064030:S100 Calcium Binding Protein G / Q000378:metabolism*; D013629:Tamoxifen / Q000494:pharmacology*; D014599:Uterus / Q000187:drug effects / Q000378:metabolism*
chemical_list: D004967:Estrogens; D064030:S100 Calcium Binding Protein G; D013629:Tamoxifen; D011374:Progesterone; D004958:Estradiol; D004964:Estriol