Insulin action on glucose uptake by soleus muscles of nonobese diabetic mice and streptozotocin diabetic mice.
To elucidate the insulin action in skeletal muscles in insulin-dependent diabetes, the glucose uptake by isolated soleus muscles was studied in streptozotocin (STZ)-diabetic mice and nonobese diabetic (NOD) mice that develop insulinopenic diabetes spontaneously. The maximally insulin-stimulated uptake of 2-deoxyglucose was significantly decreased in spontaneously diabetic NOD mice with 3 to 6 weeks duration of diabetes (10.18 +/- 0.58 v 12.80 +/- 0.44 nmol/muscle/20 min, P less than .005), while basal 2-deoxyglucose uptake was not significantly altered in them. The net insulin-stimulated uptake measured individually was also significantly reduced in spontaneously diabetic NOD mice (1.33 +/- 0.23 v 4.07 +/- 0.40 nmol/muscle/20 min, P less than .001). This was the case with STZ-diabetic ICR mice with 6 weeks duration of diabetes and STZ-diabetic NOD mice with 2 weeks and 6 weeks durations of diabetes as well. The submaximal effect of insulin on 2-deoxyglucose uptake by the soleus muscle, defined as the percent of the maximal effect achieved at 10 ng/mL of insulin, was not significantly altered in STZ-diabetic NOD mice. These data suggest that a prolonged insulinopenic state might reduce the insulin-stimulated glucose uptake in skeletal muscles by diminishing the insulin responsiveness and cause the insulin resistance in diabetic animals.
Goto
Y
Y
Department of Pediatrics, Ehime University School of Medicine, Japan.
Kida
K
K
Kaino
Y
Y
Inoue
T
T
Ikeuchi
M
M
Miyagawa
T
T
Matsuda
H
H
D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D003847:Deoxyglucose / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy / Q000378:metabolism*; D003922:Diabetes Mellitus, Type 1 / Q000188:drug therapy / Q000378:metabolism*; D005260:Female; D005947:Glucose / Q000378:metabolism*; D007328:Insulin / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009132:Muscles / Q000378:metabolism*; D012737:Sex Factors
D001786:Blood Glucose; D007328:Insulin; D003847:Deoxyglucose; D005947:Glucose