Effects of actinomycin D on the lysosomes of newborn rat hepatocytes.
The effects of actinomycin D on newborn rat liver were studied by using biochemical assays, electron microscopy, and quantitative morphometry. Actinomycin prevented the normal postnatal rise in acid a 1,4 glucosidase (maltase) activity and the breakdown of lysosomal glycogen. The results suggest that the postnasal increase in acid glucosidase activity is protein synthesis dependent. This enzyme is critical for the catabolism of the lysosomal glycogen in newborn rat hepatocytes.
Kotoulas
O B
OB
Department of Anatomy, Histology, and Embryology, Medical School, University of Ioannina, Greece.
D000818:Animals; D000831:Animals, Newborn; D003609:Dactinomycin / Q000494:pharmacology*; D065089:Glycoside Hydrolase Inhibitors; D008099:Liver / Q000201:enzymology; D008112:Liver Glycogen / Q000378:metabolism*; D008247:Lysosomes / Q000187:drug effects* / Q000648:ultrastructure; D051381:Rats; D011919:Rats, Inbred Strains; D000520:alpha-Glucosidases / Q000378:metabolism
D065089:Glycoside Hydrolase Inhibitors; D008112:Liver Glycogen; D003609:Dactinomycin; D000520:alpha-Glucosidases