title: Presentation of antigen to T lymphocytes by non-immune B-cell hybridoma clones: evidence for specific and non-specific presentation.
abstract: Non-immune SJL (H-2s) spleen cells were fused with (H-2d) Balb/c 653-myeloma cells and the hybridomas were cloned by two limiting dilutions. The resulting hybrid B- cell clones were tested for their antigen presentation capability to SJL T-cell lines that were specific for either lysozyme or myoglobin. In proliferative assays, 53% of the antigen presenting B-cell clones were able to present both myoglobin and lysozyme (general presenters) while the other 47% presented specifically either myoglobin or lysozyme (specific presenters). The ability to selectively present either myoglobin or lysozyme indicates that antigen presentation at the clonal level can be specific or non-specific depending on the particular B-cell clone.
authors.affiliation: NASA - Johnson Space Center, Houston, Texas 77058.
authors.forename: H H
authors.initials: HH
authors.lastname: Cohly
authors.forename: D R
authors.initials: DR
authors.lastname: Morrison
authors.forename: M Z
authors.initials: MZ
authors.lastname: Atassi
mesh_terms: D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology*; D006825:Hybridomas / Q000276:immunology*; D051379:Mice; D013601:T-Lymphocytes / Q000276:immunology*