Species-specific response to the rodent carcinogens 1,2-dimethylhydrazine and 1,2-dibromo-3-chloropropane in rodent bone-marrow micronucleus assays.
1,2-Dimethylhydrazine is confirmed as active in the mouse bone-marrow micronucleus assay when administered as an aqueous solution via oral gavage to three different strains of mice. It is also shown to be inactive in a corresponding rat assay under similar conditions of test. The observations were independently repeated in two laboratories. In contrast, 1,2-dibromo-3-chloropropane showed the reverse profile, being active in the rat bone-marrow assay but inactive in the mouse; the latter observations were also made in two laboratories. The carcinogen procarbazine was active in both species. These findings are discussed within the context of the present GeneTox revision of the standard test protocol of the rodent bone-marrow micronucleus assay.
Albanese
R
R
ICI Pharmaceuticals Division, Macclesfield, Cheshire, UK.
Mirkova
E
E
Gatehouse
D
D
Ashby
J
J
D019813:1,2-Dimethylhydrazine; D000818:Animals; D001853:Bone Marrow / Q000187:drug effects* / Q000648:ultrastructure; D002273:Carcinogens / Q000633:toxicity*; D002467:Cell Nucleus / Q000187:drug effects / Q000648:ultrastructure; D004127:Dimethylhydrazines / Q000633:toxicity*; D005260:Female; D008297:Male; D008763:Methylhydrazines / Q000633:toxicity*; D051379:Mice; D008815:Mice, Inbred Strains; D009152:Mutagenicity Tests; D011407:Propane / Q000031:analogs & derivatives* / Q000633:toxicity; D051381:Rats; D011919:Rats, Inbred Strains; D013045:Species Specificity
D002273:Carcinogens; D004127:Dimethylhydrazines; D008763:Methylhydrazines; C007318:1,2-dibromo-3-chloropropane; D019813:1,2-Dimethylhydrazine; D011407:Propane