title: DNA flow cytometric values in bladder carcinoma biopsies obtained from fresh and paraffin-embedded material.
abstract: DNA-histograms were obtained by flow cytometry (FCM) of nuclei prepared from fresh bladder carcinoma biopsies and from 100 micron sections of paraffin-embedded material from the same biopsies. Linear regression analysis of ploidy values from fresh material versus those from paraffin blocks showed excellent correlation (R2 = 0.957). Owing to the high background, the paraffin-embedded material was less suited for analysis of S-phase fraction. It is concluded that DNA FCM of nuclei obtained from paraffin-embedded bladder carcinoma biopsies yields reliable results concerning ploidy, but does not permit evaluation of the S-phase fraction.
authors.affiliation: Department of Medical Oncology, Norweigian Radium Hospital, Oslo, Norway.
authors.forename: A B
authors.initials: AB
authors.lastname: Jacobsen
authors.forename: S D
authors.initials: SD
authors.lastname: Fosså
authors.forename: E
authors.initials: E
authors.lastname: Thorud
authors.forename: S
authors.initials: S
authors.lastname: Lunde
authors.forename: J E
authors.initials: JE
authors.lastname: Melvik
authors.forename: E O
authors.initials: EO
authors.lastname: Pettersen
mesh_terms: D001706:Biopsy; D004273:DNA, Neoplasm / Q000032:analysis*; D005434:Flow Cytometry; D006652:Histological Techniques; D006801:Humans; D010232:Paraffin; D001749:Urinary Bladder Neoplasms / Q000032:analysis*
chemical_list: D004273:DNA, Neoplasm; D010232:Paraffin