title: Culturing and robotic harvesting of bone marrow, lymph nodes, peripheral blood, fibroblasts, and solid tumors with in situ techniques.
abstract: We have modified our in situ method for culturing amniocytes and have successfully used this new procedure to culture cells from bone marrow, lymph nodes, peripheral blood, fibroblasts, and solid tumors. We have been so satisfied with this method that we have used it in our routine practice for the past 2.5 years. A convenient feature of the in situ method is that the cultures can be processed with a commercially available robotic harvesting system (modified Tecan Robotic Sample Processor Model 505). This system also works well for in situ cultures of amniotic fluids. Robotic harvesting of in situ cultures provides a consistent and accurate procedure that saves about 10-15 minutes of technologist time per case.
authors.affiliation: Cytogenetics Laboratory, Mayo Clinic, Rochester, MN 55905.
authors.forename: J L
authors.initials: JL
authors.lastname: Spurbeck
authors.forename: R O
authors.initials: RO
authors.lastname: Carlson
authors.forename: J E
authors.initials: JE
authors.lastname: Allen
authors.forename: G W
authors.initials: GW
authors.lastname: Dewald
mesh_terms: D001773:Blood Cells / Q000166:cytology; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D003584:Cytological Techniques; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D007621:Karyotyping; D008198:Lymph Nodes / Q000166:cytology; D009369:Neoplasms / Q000473:pathology; D012371:Robotics