Light scattering measurements of the gels of gelatin and acto heavy meromyosin solutions by sample rotation method.
The gelation processes of gelatin and acto heavy meromyosin solutions were investigated by a new method of light scattering. The sample was given a constant slow rotation around a vertical axis in a typical dynamic light-scattering setup. The measurements were made on solutions in which polystyrene latex spheres were added as the scattering probes. When a sample reached the gel state, the intensity of the light scattered from the sample fluctuated highly. The relative standard deviation of the intensity fluctuation has been shown to be a good measure of gelation. In addition, computer simulations of this scattering system were found to simulate well the experimental results.
Kobayasi
S
S
Department of Molecular Biology, Faculty of Science, Nagoya University, Japan.
Kondo
H
H
Okamoto
M
M
D003198:Computer Simulation; D005780:Gelatin / Q000032:analysis*; D005782:Gels / Q000032:analysis; D008027:Light; D008956:Models, Chemical; D015879:Myosin Subfragments / Q000032:analysis*; D010446:Peptide Fragments / Q000032:analysis*; D012399:Rotation; D012542:Scattering, Radiation; D012996:Solutions / Q000032:analysis
D005782:Gels; D015879:Myosin Subfragments; D010446:Peptide Fragments; D012996:Solutions; D005780:Gelatin