Assessment of the mutagenic potential of a fungicide Bavistin using multiple assays.
The fungicide Bavistin was assessed for mutagenic potential by various assays. Bavistin was found to be unable to induce gene mutation in Salmonella typhimurium, but it was able to induce transfection inhibition in Mycobacterium smegmatis. Bavistin was able to induce immediate genotoxic effects in plants but these were not carried through in development as in the long term no genotoxic effects were observed by the progeny test. Bavistin did induce micronuclei formation and did cause an increase in the ratio of normochromatic to polychromatic erythrocytes in mice. It was able to induce a very low frequency of sister-chromatid exchange in human lymphocytes and in addition, it was observed that the chemical affected the mitotic index but did not affect the cell cycle duration. Present studies indicate that the pesticide shows a positive response in 4 out of 5 different test systems (Table 8) and most of the observations support that Bavistin is genotoxic.
Pandita
T K
TK
Microbiology and Cell Biology Laboratory, Indian Institute of Science, Bangalore.
D000383:Agriculture; D000818:Animals; D001562:Benzimidazoles / Q000633:toxicity*; D002219:Carbamates; D002453:Cell Cycle / Q000187:drug effects; D002869:Chromosome Aberrations; D003434:Crossing Over, Genetic / Q000187:drug effects; D004249:DNA Damage; D004269:DNA, Bacterial; D005659:Fungicides, Industrial / Q000633:toxicity*; D066298:In Vitro Techniques; D051379:Mice; D008940:Mitotic Index / Q000187:drug effects; D009152:Mutagenicity Tests; D009153:Mutagens; D010944:Plants; D012486:Salmonella typhimurium / Q000187:drug effects; D012854:Sister Chromatid Exchange / Q000187:drug effects
D001562:Benzimidazoles; D002219:Carbamates; D004269:DNA, Bacterial; D005659:Fungicides, Industrial; D009153:Mutagens; C006698:carbendazim