Development of a bifunctional crosslinking agent with potential for the preparation of immunotoxins.
A new protein crosslinking agent, 2,3-dibromopropionyl-N-hydroxysuccinimide ester, has been synthesized and characterized. The potential use of this compound as a temperature-controllable heterobifunctional crosslinking agent has been investigated using model systems and its reactivity compared with that of chlorambucil-N-hydroxysuccinimide ester. The coupling of 14C-labeled phenylethylamine to lysozyme has been used to illustrate the feasibility of the use of this crosslinking agent for the synthesis of immunotoxins.
McKenzie
J A
JA
Clinical Immunology Research Centre, University of Sydney, New South Wales, Australia.
Raison
R L
RL
Rivett
D E
DE
D000596:Amino Acids / Q000032:analysis; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D003432:Cross-Linking Reagents; D004591:Electrophoresis, Polyacrylamide Gel; D000922:Immunotoxins; D013388:Succinimides / Q000138:chemical synthesis*
D000596:Amino Acids; D003432:Cross-Linking Reagents; D000922:Immunotoxins; D013388:Succinimides; C060860:2,3-dibromopropionyl-N-hydroxysuccinimide ester