In situ behavior of the pyrimidine pathway enzymes in Saccharomyces cerevisiae. 3. Catalytic and regulatory properties of carbamylphosphate synthetase: channeling of carbamylphosphate to aspartate transcarbamylase.
The present work reports direct evidence for the channeling of carbamylphosphate from carbamylphosphate synthetase to aspartate transcarbamylase in the multifunctional protein that catalyzes the two first reactions of the pyrimidine pathway in Saccharomyces cerevisiae. This phenomenon is almost certainly related to the previously reported observation that the apparent in situ catalytic mechanism of aspartate transcarbamylase is altered by the association of this enzyme to carbamylphosphate synthetase. As a prerequisite of this investigation, the in situ catalytic and regulatory properties of carbamylphosphate synthetase were studied in the permeabilized cells of a strain that contains the wild-type multifunctional protein but is devoid of the carbamylphosphate synthetase specific for the arginine pathway.
Belkaïd
M
M
Laboratoire d'Enzymologie, Centre National de la Recherche Scientifique, Gif-sur-Yvette, France.
Penverne
B
B
Hervé
G
G
D000255:Adenosine Triphosphate / Q000378:metabolism; D001221:Aspartate Carbamoyltransferase / Q000378:metabolism; D001639:Bicarbonates / Q000378:metabolism; D002219:Carbamates / Q000378:metabolism*; D002223:Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing) / Q000378:metabolism*; D002221:Carbamyl Phosphate / Q000378:metabolism*; D002463:Cell Membrane Permeability; D002956:Citrulline / Q000378:metabolism; D005973:Glutamine / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008025:Ligases / Q000378:metabolism*; D011743:Pyrimidines / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology*
D001639:Bicarbonates; D002219:Carbamates; D011743:Pyrimidines; D005973:Glutamine; D002956:Citrulline; D002221:Carbamyl Phosphate; D000255:Adenosine Triphosphate; D001221:Aspartate Carbamoyltransferase; D008025:Ligases; D002223:Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing)