Simultaneous determination of mefloquine and chloroquine in biological fluids using high-performance liquid chromatography.
A high-performance liquid chromatography (HPLC) method for the simultaneous determination of mefloquine and chloroquine in biological fluids is described. Methaqualone is used as an internal standard. After a single-step extraction, achieved from alkalinized samples with diethyl ether, a separation is obtained using a mu-Bondapak phenyl column with a mobile phase of acetonitrile and sodium acetate buffer. Detection is done at 305 nm. The lower detection limits are 3 and 10 micrograms/l for mefloquine and chloroquine, respectively. Thus, this method appears to be simple, rapid, very sensitive and applicable in pharmaco-clinical, toxicological and forensic analyses.
Lamant
J M
JM
Institut de Médecine Légale, Strasbourg.
Kintz
P
P
Tracqui
A
A
Mangin
P
P
Lugnier
A A
AA
Chaumont
A J
AJ
D000962:Antimalarials / Q000097:blood*; D002738:Chloroquine / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D015767:Mefloquine; D008702:Methaqualone / Q000097:blood; D011804:Quinolines / Q000097:blood*
D000962:Antimalarials; D011804:Quinolines; D008702:Methaqualone; D002738:Chloroquine; D015767:Mefloquine