[Effects of allogeneic LAK cells on the function of immune system of the recipients].
SHR rats, 1 week of age, were i.p. inoculated with allogeneic JB LAK cells generated by 3-day incubation of spleen cells of JB rats in CM-IL 2. The spleens were removed 4 and 12 weeks later and their cells were tested for proliferative potential to mitogens and their response and production of IL2. The results demonstrated that the allo-LAK recipients showed no signs of acute or chronic GvH disease but showed immune response equivalent to or higher than the normal controls. Severe functional defects were observed in the newborn SHR rats after i.p. inoculation of allogeneic fresh spleen cells from JB rats. The results strongly suggest that allogeneic LAK cells be ineffective in terms of induction of GvH disease or GvH associated immunodeficiencies (GvHID). Therefore, this might be promising in clinical trials on cancer adoptive immunotherapy with allogeneic, instead of autologous, LAK cells.
Zhang
H T
HT
Cancer Institute, Haerbin Medical College.
D000818:Animals; D006086:Graft vs Host Disease / Q000276:immunology*; D007116:Immunization, Passive; D007376:Interleukin-2 / Q000096:biosynthesis; D007694:Killer Cells, Natural / Q000637:transplantation*; D051381:Rats; D011918:Rats, Inbred SHR; D014184:Transplantation, Homologous / Q000009:adverse effects*
D007376:Interleukin-2