[Affinity modification of Escherichia coli ribosomes with 2',3'-O-[4-(N-2-chloroethyl)-N-methylamino]-benzylidene derivative of AUGU3 in the 70S initiation complexes].
Affinity labelling of the Escherichia coli ribosomes with the 2',3'-O-[4-(N-(2-chloroethyl)-N-methylamino]benzylidene derivative of AUGU3(AUGU3[14C]CHRCl) has been studied within 70S initiation complexes ribosome.AUGU3[14C]CHRCl.fMet-tRNA(Metf) and binary complex ribosome.AUGU3[14C]CHRCl. Various ways of the 70S initiation complex formation resulted in differently labelled products. Proteins S5, S7, S9, L1, L16 were thus identified as cross-linked with AUGU3[14C]CHRCl within an initiation complex obtained in the presence of initiation factors IF-1, IF-2, IF-3, whereas only proteins S5 and S7 were cross-linked within the complex obtained with the sole factor IF-2. Proteins S1, S3, L1 and L33 were labelled within the initiation complex obtained nonenzymatically but only protein S1 within the binary complex. In all complexes formed with use of initiation factors labelling of IF-2 factor was invariably observed.
Ven'iaminova
A G
AG
Vladimirov
S N
SN
Drygl
S A
SA
Zenkova
M A
MA
Karpova
G G
GG
D000345:Affinity Labels; D000926:Anticodon; D001597:Benzylidene Compounds; D003062:Codon; D004926:Escherichia coli / Q000378:metabolism*; D009150:Mustard Compounds; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000378:metabolism*; D010448:Peptide Initiation Factors / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D012269:Ribosomal Proteins / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism*
D000345:Affinity Labels; D000926:Anticodon; D001597:Benzylidene Compounds; D003062:Codon; D009150:Mustard Compounds; D009841:Oligonucleotides; D010448:Peptide Initiation Factors; D012333:RNA, Messenger; D012269:Ribosomal Proteins
Affinnaia modifikatsiia ribosom Escherichia coli 2',3'-O-[4-(N-2-khloroétil)-N-metilamino]-benzilidenovym proizvodnym AUGU3 v 70S kompleksakh initsiatsii.