In vivo induction of malignant transformation of nude mouse B lymphocytes by a human multiple myeloma.
The bone marrow puncture fluid of human multiple myeloma was inoculated subcutaneously in BALB/C/nu nude mice. After passage of the xenograft from nude mice in vitro, a murine malignant lymphosarcoma cell line was unexpectedly established, which was named NLB-SK. The murine NLB-SK cell line has been cultivated in vitro over 72 passages for 10 months. Repeated cryopreservation showed that the murine NLB-SK cell line revived satisfactorily. Based on cell biological characteristics the malignant transformation might be attributed to horizontal oncogenesis between human malignant tumor cells and nude mouse normal somatic cells.
Gao
T X
TX
Central Laboratory of Electron Microscopy, Shandong Medical University, Jinan.
Zhou
L H
LH
Wang
Y B
YB
Zhang
W G
WG
Chen
Y H
YH
Liu
J F
JF
D000818:Animals; D001402:B-Lymphocytes / Q000473:pathology*; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D005260:Female; D006801:Humans; D008228:Lymphoma, Non-Hodgkin / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008875:Middle Aged; D009101:Multiple Myeloma / Q000473:pathology*; D009368:Neoplasm Transplantation