An improved method for the histological preparation of single hairs and dust samples--morphological and immunological examination.
An improved method of the serological and morphological investigation of human hair is reported. The hair was firmly fixed onto a microscopic slide with cellophane tape and observed microscopically to confirm the cuticula images and the presence of the medulla. A piece of the hair containing the medulla was dissected, embedded in paraffin, and a cross section of this hair was prepared. By treating the sample with immunohistochemistry (biotin-antibiotin ABC technique), the blood type of the hair was confirmed definitively. Dust containing shaved beard can be examined in the same way.
Tanabe
R
R
Department of Forensic Medicine, Faculty of Medicine, University of Tokyo, Japan.
Ishiyama
I
I
Itakura
Y
Y
D000269:Adhesives; D001788:Blood Grouping and Crossmatching / Q000379:methods; D004391:Dust; D006197:Hair / Q000032:analysis / Q000033:anatomy & histology*; D006652:Histological Techniques; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male
D000269:Adhesives; D004391:Dust