A review and analysis of the Chinese hamster ovary/hypoxanthine guanine phosphoribosyl transferase assay to determine the mutagenicity of chemical agents. A report of phase III of the U.S. Environmental Protection Agency Gene-Tox Program.
Published literature on the Chinese hamster ovary cell/hypoxanthine guanine phosphoribosyl transferase (CHO/HGPRT) assay from mid-1979 through June 1986 was reviewed and evaluated. Data from the papers considered acceptable include test results on 121 chemicals belonging to 25 chemical classes. A total of 87 chemicals were evaluated positive, 3 negative, and 31 inconclusive. Mutagenicity data on 49 of the 121 chemicals evaluated could also be compared with in vivo animal carcinogenicity data. 40 of the 43 reported animal carcinogens were considered mutagenic. Caprolactam, the only definitive noncarcinogen in the group of 49, was not mutagenic. The CHO/HGPRT assay was concluded to be an appropriate assay system for use in the screening of chemicals for genotoxicity.
Li
A P
AP
Monsanto Company, St. Louis, MO 63167.
Gupta
R S
RS
Heflich
R H
RH
Wassom
J S
JS
D000818:Animals; D001711:Biotransformation; D002273:Carcinogens; D006224:Cricetinae; D003412:Cricetulus; D005069:Evaluation Studies as Topic; D005260:Female; D007041:Hypoxanthine Phosphoribosyltransferase; D009152:Mutagenicity Tests / Q000592:standards; D010053:Ovary / Q000187:drug effects; D014481:United States; D014484:United States Environmental Protection Agency
D002273:Carcinogens; D007041:Hypoxanthine Phosphoribosyltransferase