Simulation of all-or-none K+ efflux from yeast provoked by xenobiotics.
In experiments dealing with the effect of xenobiotics upon the efflux of K+ from yeast cells, one should be aware that when this efflux proceeds via an all-or-none process, the K+ being released from the intoxicated cells can again be accumulated into the still unaffected cells. Therefore, the measured net efflux of K+ will be less than the efflux from the intoxicated cells. The difference between these two magnitudes can be minimalized by incubating the cells for only a short period and on applying yeast densities that are not too high. When the cells are permeabilized relatively slowly but ultimately to a great extent, the kinetics of K+ efflux may be quite complicated.
Borst-Pauwels
G W
GW
Laboratory of Chemical Cytology, Roman Catholic University, Toernooiveld, Nijmegen, The Netherlands.
D000465:Algorithms; D002463:Cell Membrane Permeability; D007700:Kinetics; D011188:Potassium / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000378:metabolism; D012984:Software
D011188:Potassium