Removal of surface adherent Staphylococcus aureus in the determination of phagocytosis and intracellular killing by the use of lysostaphin.
Use of lysostaphin to remove non-phagocytized, granulocyte-adherent bacteria allows more precise calculation of the percentage of the ingested as well as killed bacteria in the process of phagocytosis.
Steuden
W
W
Isotopic Laboratory, Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław.
Szymaniec
S
S
D000818:Animals; D002462:Cell Membrane / Q000382:microbiology; D006168:Guinea Pigs; D008248:Lysostaphin; D010587:Phagocytosis; D013211:Staphylococcus aureus
D008248:Lysostaphin