Immunoassays with time-resolved fluorescence spectroscopy: principles and applications.
This review deals with the application of time-resolved fluorescence spectroscopy in the field of immunoassay. The advantages of time-resolved fluorescence over conventional fluorometry are discussed and it is explained why the fluorescent lanthanide chelates, and especially the Eu3+ chelates, are the labels of choice in such applications. A brief description of commercially available time-resolved fluorometers is given. The principles of phase-resolved fluorometry are described. The relative merits and disadvantages of the two time-resolved assay principles currently available commercially are critically discussed. General assay designs by using either Eu3+ or a new Eu3+ chelate as the label are also discussed in some detail. A personal view of future trends in the field of immunoassay concludes this review.
Diamandis
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CyberFluor Inc., Toronto, Ontario, Canada.
D005455:Fluorescent Antibody Technique / Q000295:instrumentation*; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation*; D007202:Indicators and Reagents
D007202:Indicators and Reagents