title: Colocalization of F-actin and 34-kilodalton actin bundling protein in Dictyostelium amoebae and cultured fibroblasts.
abstract: The Ca2+-sensitive actin-binding protein isolated from Dictyostelium discoideum, 30,000-D protein (Fechheimer and Taylor: J. Biol. Chem. 259:4514-4520, 1984;) has recently been localized in filipodia of substrate-adhered amoebae (Fechheimer: J. Cell Biol. 104:1539-1551, 1987). We have determined that this protein has a Mr of 34,000 daltons and is strictly colocalized with actin filaments in both substrate-attached Dictyostelium amoebae and cultured fibroblasts. 3T3 fibroblasts, as well as normal and virally transformed rat kidney fibroblasts (NRK) contain a 34-kilodalton (kD) protein that cross-reacts specifically with antibody to the Dictyostelium bundling protein. Mammalian 34-kD protein is colocalized with F-actin in stress fibers and the cortical cytoskeleton in substrate-adhered fibroblasts. In substrate-adhered vegetative Dictyostelium, F-actin and 34-kD protein are concentrated in regions of the cell cortex exhibiting filipodia and membrane ridges. Multiple filipodia formed after exposure to the chemoattractant folic acid stain intensely for 34-kD protein, implying participation in the assembly of actin bundles during filipod formation. The cortex of pseudopodia also contained high concentrations of bundling protein, but pseudopod interiors did not. In contrast to vegetative Dictyostelium, F-actin and 34-kD protein were not colocalized in cells that had progressed through the developmental cycle. In fruiting bodies, 34-kD protein was detected by immunofluorescence microscopy only in prespore cells, while F-actin appeared in stalk cells and spores.
authors.affiliation: Department of Cell Biology and Anatomy, Cornell University Medical College, New York, New York 10021.
authors.forename: J A
authors.initials: JA
authors.lastname: Johns
authors.forename: A M
authors.initials: AM
authors.lastname: Brock
authors.forename: J D
authors.initials: JD
authors.lastname: Pardee
mesh_terms: D000199:Actins / Q000032:analysis*; D000818:Animals; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000032:analysis*; D004023:Dictyostelium / Q000032:analysis* / Q000254:growth & development / Q000648:ultrastructure; D004797:Enzyme-Linked Immunosorbent Assay; D005347:Fibroblasts; D005455:Fluorescent Antibody Technique; D008840:Microfilament Proteins / Q000032:analysis* / Q000302:isolation & purification; D008970:Molecular Weight; D011554:Pseudopodia / Q000032:analysis*; D011817:Rabbits
chemical_list: D000199:Actins; D008840:Microfilament Proteins; C052523:actin filament bundling proteins