Sensitivity of fructose-1,6-biphosphatase from yeast, liver and skeletal muscle to fructose-2,6-biphosphate and 5'-adenosine monophosphate.
As a prerequisite for future studies on the possible effect of sulphite, an anti-microbial agent, on gluconeogenesis in yeast, a comparative study of fructose-1,6-bisphosphatase (FBPase), a key enzyme of gluconeogenesis, from yeast, liver and skeletal muscle is reported. In contrast to FBPase from yeast or liver, FBPase from skeletal muscle is approximately 1000-fold more sensitive to inhibition by 5' adenosine monophosphate and 30 to 250-fold less sensitive to inhibition by fructose-2,6-bisphosphate. The kinetic properties of the FBPases, determined by the ratios R(Mg2+/Mn2+) and R (pH 7/9) of the enzyme activities, measured at 10 mM Mg2+ and 2 mM Mn2+ and at pH 7.0 and 9.0, respectively, show a drastic difference between the skeletal muscle and the yeast or liver enzymes. The data support the idea that the enzymes from yeast and liver function in gluconeogenesis, whereas the enzyme from skeletal muscle is involved in other biological functions.
von Herrath
M
M
Biochemisches Institut der Universität Freiburg, Federal Republic of Germany.
Holzer
H
H
D000249:Adenosine Monophosphate / Q000494:pharmacology*; D000818:Animals; D005635:Fructosediphosphates / Q000494:pharmacology*; D005943:Gluconeogenesis / Q000187:drug effects; D006598:Hexosediphosphates / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects*; D008274:Magnesium / Q000494:pharmacology; D008345:Manganese / Q000494:pharmacology; D009132:Muscles / Q000187:drug effects*; D010447:Peptide Hydrolases; D011480:Protease Inhibitors; D011817:Rabbits; D012441:Saccharomyces cerevisiae / Q000187:drug effects*
D005635:Fructosediphosphates; D006598:Hexosediphosphates; D011480:Protease Inhibitors; D000249:Adenosine Monophosphate; D008345:Manganese; C027652:fructose 2,6-diphosphate; D010447:Peptide Hydrolases; C020152:fructose 1,6-bisphosphatase converting enzyme; D008274:Magnesium