A synthetic peptide to the predicted 6.9K translation product of the HindIII-H/EcoRI-D region of the AcNPV genome induces antibodies to the basic DNA-binding protein.
The sequence of the HindIII-H/EcoRI-D region of the AcNPV genome revealed a 55 codon methionine-initiated open reading frame (Wilson, M.E., Mainprize, T.H., Friesen, P.D. and Miller, L.K., 1987, J. Virol. 61, 661-666). This open reading frame predicts a 6.9K translation product with an amino acid composition nearly identical to the basic virion DNA-binding protein. A synthetic peptide was prepared to a region of the predicted 6.9K protein and used to induce antibodies in rabbits. Western blots of viral proteins, infected cell-specific proteins (24 h p.i.), and basic chromatin-associated proteins (24 h p.i.) were probed with anti-6.9K antisera. In all immunoblots, the anti-6.9K antisera were specific for the basic DNA-binding protein. This investigation provides further evidence that the basic DNA-binding protein of the virion is a viral-encoded gene transcribed from the HindIII-H/EcoRI-D region of the genome.
Wilson
M E
ME
Department of Entomology, University of Kentucky, Lexington 40546-0091.
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis* / Q000276:immunology; D004268:DNA-Binding Proteins / Q000235:genetics / Q000276:immunology*; D007304:Insect Viruses / Q000235:genetics / Q000276:immunology*; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000276:immunology; D011817:Rabbits; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology*
D000914:Antibodies, Viral; D004268:DNA-Binding Proteins; D010446:Peptide Fragments; D014764:Viral Proteins