Study of the coinduction by fatty acids of catalase A and acyl-CoA oxidase in standard and mutant Saccharomyces cerevisiae strains.
Evidence is presented that Saccharomyces cerevisiae can metabolize fatty acids via the inducible peroxisomal beta-oxidation pathway even when these acids are not the sole carbon source. The fatty acids of chain length of C10-C18 induce acyl-CoA oxidase simultaneously with catalase A but have no effect on catalase T and acyl-CoA dehydrogenase. The coinduction of both acyl-CoA oxidase and catalase A is recorded in strains with both active catalase A and T or displaying only catalase A activity. In mutants lacking catalase A, the induction of acyl-CoA oxidase is observed without a concomitant increase in catalase activity. After centrifugation in a linear Ficoll gradient of the particulate fraction from the cells grown on ethanol and oleate the activity of acyl-CoA oxidase cosediments with catalase A. The relationship of catalase A to acyl-CoA oxidase is discussed.
Skoneczny
M
M
Institute of Biochemistry and Biophysics, Polish Academy of Science, Warsaw.
Chełstowska
A
A
Rytka
J
J
D042962:Acyl-CoA Oxidase; D002374:Catalase / Q000096:biosynthesis* / Q000235:genetics; D002499:Centrifugation, Density Gradient; D004790:Enzyme Induction / Q000187:drug effects; D005227:Fatty Acids / Q000494:pharmacology*; D008830:Microbodies / Q000201:enzymology; D009154:Mutation; D010088:Oxidoreductases / Q000096:biosynthesis* / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins
D005227:Fatty Acids; D029701:Saccharomyces cerevisiae Proteins; D010088:Oxidoreductases; D002374:Catalase; D042962:Acyl-CoA Oxidase; C467991:POX1 protein, S cerevisiae