Use of the avidin-biotin peroxidase system to detect rabies antigen in formalin-fixed paraffin-embedded tissues.
We stained rabies-infected nervous and salivary-gland tissues fixed in formalin or acetone and embedded in paraffin with the avidin-biotin peroxidase system. With this system, rabies-virus antigen was detected in neurons, glandular acinar cells, and vascular endothelial cells more effectively than by immunofluorescence, especially when tissues were enzyme-digested with pronase before immunoperoxidase staining. The avidin-biotin peroxidase system should be useful for routine diagnosis, retrospective studies of rabies, and identification of specific cells involved in the spread of virus in rabies-infected hosts.
Fekadu
M
M
Center for Infectious Diseases, Centers for Disease Control, Atlanta, Georgia.
Greer
P W
PW
Chandler
F W
FW
Sanderlin
D W
DW
D000818:Animals; D000956:Antigens, Viral / Q000302:isolation & purification*; D001360:Avidin; D001710:Biotin; D004730:Endothelium, Vascular / Q000276:immunology; D005557:Formaldehyde; D006801:Humans; D007124:Immunoenzyme Techniques; D009474:Neurons / Q000276:immunology; D010232:Paraffin; D011818:Rabies / Q000175:diagnosis; D011820:Rabies virus / Q000276:immunology*; D012469:Salivary Glands / Q000276:immunology
D000956:Antigens, Viral; D001360:Avidin; D005557:Formaldehyde; D001710:Biotin; D010232:Paraffin