Major histocompatibility complex gene product expression on pancreatic beta cells in acutely diabetic BB rats.
Type I diabetes mellitus was induced in young, diabetes-prone BB rats by the passive transfer of concanavalin A-activated T lymphocytes from the spleens of acutely diabetic BB rats. The pancreas of the recipients was examined 1-2 days after the onset of glycosuria by immunocytochemistry by means of monoclonal antibodies for determining whether 1) Class I and/or II major histocompatibility gene complex (MHC) products were expressed on beta cells and 2) the mononuclear cell infiltrates were represented by T cells. Marked expression of Class I MHC gene products was evident on beta cells. In contrast, Class II MHC gene products were not identified on normal-appearing beta cells. Dendritic cells dispersed throughout the acinar and interstitial pancreas were markedly increased in number. The mononuclear cell infiltrate contained few cells (1-15%) recognized by a pan-T cell marker. Although it is possible that this passive transfer model might differ considerably from the spontaneously occurring diabetic state in the rat, this study suggests that 1) Class I, rather than Class II, MHC gene expression may be pivotal to beta-cell injury in diabetic rats, and 2) non-T cells may constitute an effector cell population central to beta-cell necrosis in Type I diabetes mellitus.
Issa-Chergui
B
B
Department of Pathology, Montreal Children's Hospital, Québec, Canada.
Yale
J F
JF
Vigeant
C
C
Seemayer
T A
TA
D000818:Animals; D000911:Antibodies, Monoclonal; D003921:Diabetes Mellitus, Experimental / Q000276:immunology* / Q000473:pathology; D003922:Diabetes Mellitus, Type 1 / Q000276:immunology / Q000473:pathology; D005805:Genes, MHC Class I; D006649:Histocompatibility Antigens / Q000032:analysis*; D007116:Immunization, Passive; D007249:Inflammation; D007515:Islets of Langerhans / Q000276:immunology* / Q000473:pathology; D008285:Major Histocompatibility Complex; D051381:Rats; D011913:Rats, Inbred BB; D013601:T-Lymphocytes / Q000276:immunology / Q000637:transplantation; D014158:Transcription, Genetic
D000911:Antibodies, Monoclonal; D006649:Histocompatibility Antigens