Cytotoxic activity of an interleukin 6-Pseudomonas exotoxin fusion protein on human myeloma cells.
A chimeric toxin composed of human interleukin 6 (IL-6) attached to a portion of Pseudomonas exotoxin (PE) devoid of its own cell recognition domain has been produced in Escherichia coli. The fusion protein (IL-6-PE40) is cytotoxic to a human myeloma cell line expressing IL-6 receptors but has no effect on IL-6 receptor-negative cells. The specificity of IL-6-PE40 cytotoxicity was demonstrated through competition with excess IL-6 and neutralization with an antibody to IL-6. IL-6-PE40 may be useful in the selective elimination of myeloma cells and other cells with high numbers of IL-6 receptors.
Siegall
C B
CB
National Institutes of Health, Laboratory of Molecular Biology, Bethesda, MD 20892.
Chaudhary
V K
VK
FitzGerald
D J
DJ
Pastan
I
I
D036002:ADP Ribose Transferases; D001427:Bacterial Toxins; D001483:Base Sequence; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D005098:Exotoxins; D006801:Humans; D015850:Interleukin-6; D007378:Interleukins; D008969:Molecular Sequence Data; D009101:Multiple Myeloma / Q000473:pathology*; D010957:Plasmids; D011971:Receptors, Immunologic / Q000378:metabolism; D019947:Receptors, Interleukin-6; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000187:drug effects; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A
D036002:ADP Ribose Transferases; D001427:Bacterial Toxins; D005098:Exotoxins; D015850:Interleukin-6; D007378:Interleukins; D011971:Receptors, Immunologic; D019947:Receptors, Interleukin-6; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A