Reno-submandibular axis controls release of extrarenal inactive renin.
The mechanisms causing the release of plasma inactive renin (PIR) area still unclear. We have investigated the role of the kidney in the release of trypsin-activable PIR from extrarenal sources in the rat, with special reference to the submandibular gland. The activation of PIR was performed by incubation with 20 mg/ml trypsin at 4 degrees C for up to 10 min; the reaction was then terminated by addition of 20 mg/ml of soybean trypsin inhibitor. Bilateral nephrectomy resulted in a gradual, marked, sex-independent increase in PIR concentration, reaching levels 4.5 times higher than basal in 24 h (time 0: 14.8 +/- 1.0 ng/ml per h; 24 h: 66.8 +/- 3.4 ng/ml per h, mean +/- s.d., P less than 0.001). This increase was not altered by the concomitant intravenous infusion of pressor doses of either angiotensin (Ang) II (30 ng/min) or pure mouse submandibular renin (a 20-ng intravenous bolus followed by intravenous infusion at the rate of 50 ng/h) for 4 h, but was completely prevented by prior removal of the submandibular glands, in which no activity of active renin and no inactive renin was detected. These results suggest that the post-nephrectomy increase of PIR is not dependent on feedback mechanisms of the suppressed renin-angiotensin system, but is controlled by the presence of submandibular glands in the rat.
Miyazaki
S
S
Third Department of Internal Medicine, Gifu University School of Medicine, Tsukasa-machi, Japan.
Sakanaka
A
A
Chimori
K
K
Kosaka
J
J
Goi
R
R
Dodo
S
S
Miura
K
K
Suzuki
F
F
D000818:Animals; D004789:Enzyme Activation / Q000187:drug effects; D004792:Enzyme Precursors / Q000097:blood / Q000378:metabolism*; D005260:Female; D007668:Kidney / Q000502:physiology*; D008297:Male; D008970:Molecular Weight; D009392:Nephrectomy; D051381:Rats; D011919:Rats, Inbred Strains; D012083:Renin / Q000097:blood / Q000378:metabolism*; D013363:Submandibular Gland / Q000502:physiology*; D014357:Trypsin / Q000494:pharmacology
D004792:Enzyme Precursors; D014357:Trypsin; D012083:Renin