Role of the cytochrome b6.f complex in the redox-controlled activity of Acetabularia thylakoid protein kinase.
The regulation of the protein kinase activity responsible for the phosphorylation of the light-harvesting complex of photosystem II (LHCII) 27-kDa polypeptide involved in the State I-State II transitions in Acetabularia thylakoids was investigated. The LHCII kinase of isolated thylakoids retains its activity in absence of light-driven electron flow or reductants added in the dark. However, the kinase is reversibly inactivated by addition of oxidants in vitro or by far red (710 nm) light in vivo. Inhibitors of the quinol oxidase site of the cytochrome b6.f complex inactivate the LHCII kinase in the dark, and also in the light, or in presence of duroquinol when the plastoquinone pool is reduced. Inhibitors of the quinone reductase site of the b6.f complex have practically no effect in the dark and stimulate the kinase activity in the light. Based on these data and on our previous report, showing specific loss of LHCII kinase activity in a Lemna mutant lacking the cytochrome b6.f complex (Gal, A., Shahak, Y., Schuster, G., and Ohad, I. (1987) FEBS Lett. 221, 205-210), we propose that the activity of the LHCII kinase is regulated by the redox state of a cytochrome b6.f complex component(s) which responds to the balance of electron flow from photosystem II via the plastoquinone pool to photosystem I.
Gal
A
A
Department of Biological Chemistry, Hebrew University of Jerusalem, Israel.
Schuster
G
G
Frid
D
D
Canaani
O
O
Schwieger
H G
HG
Ohad
I
I
D000076:Acetabularia / Q000201:enzymology*; D001280:Atrazine / Q000494:pharmacology; D016227:Benzoquinones; D002734:Chlorophyll / Q000378:metabolism; D000460:Chlorophyta / Q000201:enzymology*; D003580:Cytochromes / Q000378:metabolism*; D045348:Cytochromes f; D004237:Diuron / Q000494:pharmacology; D007700:Kinetics; D045342:Light-Harvesting Protein Complexes; D010084:Oxidation-Reduction; D010766:Phosphorylation; D045322:Photosynthetic Reaction Center Complex Proteins; D045331:Photosystem I Protein Complex; D045332:Photosystem II Protein Complex; D010940:Plant Proteins / Q000378:metabolism; D010971:Plastoquinone / Q000378:metabolism; D011494:Protein Kinases / Q000378:metabolism*; D011809:Quinones / Q000494:pharmacology
D016227:Benzoquinones; D003580:Cytochromes; D045342:Light-Harvesting Protein Complexes; D045322:Photosynthetic Reaction Center Complex Proteins; D045331:Photosystem I Protein Complex; D045332:Photosystem II Protein Complex; D010940:Plant Proteins; D011809:Quinones; D002734:Chlorophyll; D045348:Cytochromes f; D004237:Diuron; D011494:Protein Kinases; C120337:light-harvesting complex II kinase; D010971:Plastoquinone; D001280:Atrazine; C034257:duroquinone