XY follicle cells in ovaries of XX----XY female mouse chimaeras.
Oocytes with adhering follicle cells were sampled from ovaries obtained from 11 GPI-1A----GPI-1B chimaeras, comprising 10 females and 1 hermaphrodite. GPI analysis of individual oocytes revealed a marked bias towards the GPI-1B component in the germ line of this chimaeric combination. GPI-1B XY oocytes were identified in the ovary from the hermaphrodite, the bias towards the GPI-1B germ line perhaps helping to counterbalance the normally severe selection against XY oocytes. GPI analysis of follicle cells revealed a much more balanced contribution of the two components to this ovarian cell type. Importantly, GPI-1A follicle cells were identified in more than half the follicles from an XX----XY female in which the GPI-1A component was XY, supporting an earlier conclusion of Ford et al. (1974) that XY cells can contribute to the follicles of XX----XY female mice. It is suggested that XY cells can be recruited to form follicle cells in XX----XY chimaeras when there is a developmental mismatch between the two components, such that an ovary-determining signal produced by the XX component pre-empts the testis-determining action of the Y.
Burgoyne
P S
PS
MRC Mammalian Development Unit, London, UK.
Buehr
M
M
McLaren
A
A
D000818:Animals; D001854:Bone Marrow Cells; D002678:Chimera; D012734:Disorders of Sex Development / Q000473:pathology; D005260:Female; D005819:Genetic Markers; D008677:Metaphase; D051379:Mice; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA; D006080:Ovarian Follicle / Q000166:cytology; D010053:Ovary / Q000502:physiology*; D012730:Sex Chromosomes; D014178:Translocation, Genetic
D005819:Genetic Markers