Two-color flow cytometry analysis of activated T-lymphocyte subsets in type I diabetes mellitus.
We addressed the question of whether newly diagnosed type I (insulin-dependent) diabetes mellitus patients showed an increased number of DR (la+) T-lymphocytes compared with nondiabetic siblings and normal control subjects. Two-color flow cytometry measurements of peripheral-blood lymphocytes showed a slight but statistically significant increase in DR+ T-lymphocytes in diabetic subjects as well as the nondiabetic sibling control compared with the normal control subjects. This difference was not present in long-term-diabetic subjects. Thus, in addition to minor changes in this lymphocyte subset in peripheral blood, the sibling data demonstrate a lack of specificity for the disease; therefore, these measurements are probably of limited diagnostic usefulness.
Legendre
C M
CM
Transplantation Service, Royal Victoria Hospital, Montreal, Quebec, Canada.
Schiffrin
A
A
Weitzner
G
G
Colle
E
E
Guttmann
R D
RD
D000293:Adolescent; D000328:Adult; D000911:Antibodies, Monoclonal; D002648:Child; D002675:Child, Preschool; D003116:Color; D003922:Diabetes Mellitus, Type 1 / Q000235:genetics / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D005455:Fluorescent Antibody Technique; D006684:HLA-DR Antigens / Q000276:immunology; D006801:Humans; D008213:Lymphocyte Activation; D008297:Male; D013601:T-Lymphocytes / Q000145:classification*; D013997:Time Factors
D000911:Antibodies, Monoclonal; D006684:HLA-DR Antigens