title: Identification by fast atom bombardment mass spectrometry of insulin fragments produced by insulin proteinase.
abstract: We describe the isolation by reversed-phase h.p.l.c. of a number of products of the degradation of insulin by insulin proteinase and their direct analysis by fast atom bombardment mass spectrometry (f.a.b.-m.s.). Various semisynthetically labelled insulins were used, including [[2H2]GlyA1]insulin and [18O]LysB29]insulin. The results obtained confirm and extend the results obtained by non-mass-spectrometric methods [Davies, Muir, Rose & Offord (1988) Biochem. J. 249, 209-214, and papers cited therein]. Cleavage sites were identified between positions A13-A14, A14-A15, B9-B10, B13-B14, B24-B25 and B25-B26. The advantages and disadvantages of the application of f.a.b.-m.s. to such studies are discussed.
authors.affiliation: Département de Biochimie Médicale, Centre Médical Universitaire, Geneva, Switzerland.
authors.forename: L A
authors.initials: LA
authors.lastname: Savoy
authors.forename: R M
authors.initials: RM
authors.lastname: Jones
authors.forename: S
authors.initials: S
authors.lastname: Pochon
authors.forename: J G
authors.initials: JG
authors.lastname: Davies
authors.forename: A V
authors.initials: AV
authors.lastname: Muir
authors.forename: R E
authors.initials: RE
authors.lastname: Offord
authors.forename: K
authors.initials: K
authors.lastname: Rose
mesh_terms: D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D007328:Insulin / Q000031:analogs & derivatives*; D013058:Mass Spectrometry; D010446:Peptide Fragments / Q000032:analysis*
chemical_list: D007328:Insulin; D010446:Peptide Fragments