A comparative study of the cytotoxic and genotoxic effects of cisplatin and its analogue, TNO-6, in yeast.
The antitumour drug cisplatin and its analogue, TNO-6, were studied for their cytotoxic, mutagenic and recombinagenic effects in a diploid strain (D7) of the yeast, Saccharomyces cerevisiae. It was observed that the structural change in TNO-6 resulted in reduced cytotoxicity and recombinagenicity (mitotic gene conversion) but increased mutagenic activity compared to the effects of cisplatin at equimolar concentrations. These results indicated that the mechanism through which TNO-6 damages cellular DNA is different from that of cisplatin.
Hannan
M A
MA
Department of Biological and Medical Research, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia.
D002945:Cisplatin / Q000494:pharmacology*; D003432:Cross-Linking Reagents / Q000494:pharmacology; D004249:DNA Damage; D004271:DNA, Fungal / Q000187:drug effects; D005785:Gene Conversion / Q000187:drug effects; D009944:Organoplatinum Compounds / Q000494:pharmacology*; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics
D003432:Cross-Linking Reagents; D004271:DNA, Fungal; D009944:Organoplatinum Compounds; C040757:spiroplatin; D002945:Cisplatin