Constitutive c-myc expression enhances the response of murine mast cells to IL-3, but does not eliminate their requirement for growth factors.
An interleukin-3 (IL-3) dependent mast cell line (MC) was infected with a recombinant retrovirus expressing the proto-oncogene c-myc and the drug selectable marker neo. Cells containing the transcriptionally activated c-myc gene displayed an increased growth rate in liquid culture and a higher cloning efficiency in soft agar when compared to control virus infected cells. All infected cells remained absolutely dependent on IL-3 for growth and were not tumorigenic in nude mice. Similar results were obtained with two additional IL-3 dependent cell lines, the mast cell 32D and the pre-B-cell Ea3. Thus, while constitutive expression of c-myc potentiates the response of mast cells to IL-3, it is not sufficient to eliminate their requirement for growth factors.
Hume
C R
CR
Sloan Kettering Division, Graduate School of Medical Sciences, Cornell University, New York, New York 10021.
Nocka
K H
KH
Sorrentino
V
V
Lee
J S
JS
Fleissner
E
E
D000818:Animals; D002455:Cell Division; D005786:Gene Expression Regulation; D006133:Growth Substances / Q000494:pharmacology*; D007377:Interleukin-3 / Q000494:pharmacology*; D008407:Mast Cells / Q000166:cytology* / Q000187:drug effects; D051379:Mice; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics
D006133:Growth Substances; D007377:Interleukin-3; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger