Optical and electrochemical detection of DNA.
There is a growing demand for the production of a DNA biosensor with applications in medicine, the food industry, agriculture, veterinary science and environmental science. In this paper we describe methods for the optical and electrochemical detection of DNA using the enzyme horseradish peroxidase (EC 1.11.1.7) and glucose oxidase (EC 1.1.3.4). We have used bis-methylacridinium nitrate and luminol for the optical detection of DNA using a purpose built, inexpensive luminometer. Using this system detection limits of 10(-11) g of plasmid DNA have been observed. Electrochemical detection of DNA was carried out by the use of a fluoride ion selective electrode and stripping voltammetry. DNA was detected down to 10(-9)-10(-10) g of DNA by the enzymatic release of halogen ions from organohalogen compounds.
Downs
M E
ME
Biotechnology Centre, Cranfield Institute of Technology, Bedford, UK.
Warner
P J
PJ
Turner
A P
AP
Fothergill
J C
JC
D000818:Animals; D004247:DNA / Q000032:analysis*; D004563:Electrochemistry; D005949:Glucose Oxidase; D006735:Horseradish Peroxidase; D055095:Optics and Photonics / Q000295:instrumentation; D011199:Potentiometry
D004247:DNA; D005949:Glucose Oxidase; D006735:Horseradish Peroxidase