title: Cloning of the gene, speB, for streptococcal pyrogenic exotoxin type B in Escherichia coli.
abstract: The structural gene encoding streptococcal pyrogenic exotoxin type B, designated speB, was cloned in Escherichia coli and localized onto a 4.5-kilobase BamHI-BglII DNA fragment. Streptococcal pyrogenic exotoxin type B, partially purified from E. coli clones, was immunologically related to streptococcus-derived toxin. Also, toxin derived from either E. coli or Streptococcus pyogenes had similar lymphocyte mitogenic activity and molecular weight (29,300) and displayed comparable microheterogeneity when evaluated by isoelectric focusing.
authors.affiliation: Department of Microbiology, Medical School, University of Minnesota, Minneapolis 55455.
authors.forename: G A
authors.initials: GA
authors.lastname: Bohach
authors.forename: A R
authors.initials: AR
authors.lastname: Hauser
authors.forename: P M
authors.initials: PM
authors.lastname: Schlievert
mesh_terms: D001426:Bacterial Proteins; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics*; D005098:Exotoxins / Q000235:genetics*; D008565:Membrane Proteins; D011749:Pyrogens / Q000235:genetics; D013297:Streptococcus pyogenes / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D005098:Exotoxins; D008565:Membrane Proteins; D011749:Pyrogens; C117980:SpeA protein, Streptococcus pyogenes; C027951:erythrogenic toxin