title: Hexadecane-induced skin hyperplasia in the hairless rat: time course of histological and biochemical events related to the synthesis of polyamines and DNA.
abstract: Several biochemical parameters including ornithine decarboxylase activity (ODC) and tissue polyamine levels were measured during the hexadecane-induced epidermal hyperplasia of hairless rat skin. Animals received three applications of 200 microliters pure n-hexadecane on day 1. ODC activity and polyamine levels (putrescine, spermidine and spermine) in the epidermis were significantly increased and reached maximum elevations at 12 h after the start of n-hexadecane treatment with DNA synthesis peaking at 24 h. Histological studies confirmed a significant cellular edema at 24 h after the beginning of the treatment followed at 48 h by an epidermal hyperplasia which was maximum at 72 h. These data support the view that ODC activation, increased biosynthesis of polyamines and DNA are early events in epidermal cell hyperproliferation.
authors.affiliation: Centre International de Recherches Dermatologiques (CIRD), Sophia Antipolis, Valbonne, France.
authors.forename: M
authors.initials: M
authors.lastname: Bouclier
authors.forename: B
authors.initials: B
authors.lastname: Shroot
authors.forename: G
authors.initials: G
authors.lastname: Milano
authors.forename: J
authors.initials: J
authors.lastname: Ferracin
authors.forename: A
authors.initials: A
authors.lastname: Chatelus
authors.forename: C N
authors.initials: CN
authors.lastname: Hensby
mesh_terms: D000473:Alkanes / Q000633:toxicity*; D000818:Animals; D015317:Biogenic Polyamines / Q000096:biosynthesis*; D004247:DNA / Q000096:biosynthesis*; D006965:Hyperplasia; D008297:Male; D009955:Ornithine Decarboxylase / Q000378:metabolism; D051381:Rats; D012867:Skin / Q000378:metabolism / Q000473:pathology*; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors
chemical_list: D000473:Alkanes; D015317:Biogenic Polyamines; D004247:DNA; D009955:Ornithine Decarboxylase; C007932:n-hexadecane; D013936:Thymidine