Interaction of murine progesterone receptors with specific monoclonal antibodies to the avian progesterone receptor.
Monoclonal antibodies raised against purified chicken progesterone receptor (PgR) have been described and characterized recently. In this study we have screened these antibodies for cross-reactivity with murine PgR. Of the six anti-PgR antibodies tested, one (alpha PR6) precipitates murine PgR in an assay using protein A-sepharose as an absorbent for the antibody. The antibody is specific for PgR and does not react with the estrogen receptor or the glucocorticoid receptor in the same cytosol. In immunoblot experiments, both alpha PR6 and alpha PR11 recognize a 115,000 Da protein, however, alpha PR11 gives a weaker signal than alpha PR6. In photoaffinity labeling experiments, a 115,000 Da and an 83,000 Da protein covalently bind tritiated R5020 in a receptor-specific way. We conclude that the alpha PR6 antibody can be used as a tool to study the structure and function of the murine PgR.
Schneider
W
W
Lady Davis Institute for Medical Research, Sir Mortimer B. Davis Jewish General Hospital, Montreal, Quebec, Canada.
Toft
D O
DO
Sullivan
W P
WP
Shyamala
G
G
D000345:Affinity Labels; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D000941:Antigens / Q000276:immunology; D002499:Centrifugation, Density Gradient; D002645:Chickens; D003600:Cytosol / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D007124:Immunoenzyme Techniques; D007163:Immunosorbent Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D010777:Photochemistry; D051381:Rats; D011919:Rats, Inbred Strains; D011980:Receptors, Progesterone / Q000032:analysis / Q000276:immunology*; D013045:Species Specificity; D014599:Uterus / Q000032:analysis
D000345:Affinity Labels; D000911:Antibodies, Monoclonal; D000941:Antigens; D011980:Receptors, Progesterone