[Vaseline-peptone mixture as a stimulant of ascites formation in mouse hybridoma carriers].
A preliminary injection of vaseline oil with 3% of peptone in a dose of 0.5 ml induced ascitic tumors with a high taking rate in mice used as recipients of hybridomas. The dependence of the amount of ascitic fluid produced in such animals and the content of monoclonal antibodies on the time of the preliminary injection of the above-mentioned mixture, the number of cells introduced into the recipient animal, and the time of ascites collection has been studied. The optimal scheme of obtaining ascites with the use of the proposed mixture is presented. The comparison of the mixture with other stimulants of ascites production has been made.
Baryshnikov
A Iu
AIu
Trofimova
M N
MN
Novikov
V V
VV
Polianskaia
G A
GA
Sandova
O M
OM
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000302:isolation & purification; D001202:Ascitic Fluid / Q000139:chemically induced* / Q000166:cytology / Q000276:immunology; D005260:Female; D005455:Fluorescent Antibody Technique; D005620:Freund's Adjuvant / Q000494:pharmacology; D006825:Hybridomas / Q000187:drug effects* / Q000276:immunology / Q000637:transplantation; D007114:Immunization; D051379:Mice; D008807:Mice, Inbred BALB C; D010461:Peptones / Q000494:pharmacology*; D010577:Petrolatum / Q000494:pharmacology*; D013268:Stimulation, Chemical; D013729:Terpenes / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D010461:Peptones; D013729:Terpenes; C009042:pristane; D010577:Petrolatum; D005620:Freund's Adjuvant
Vazelin-peptonovaia smes' kak stimuliator obrazovaniia astsita u mysheĭ-nositeleĭ gibridom.