Saccharomyces cerevisiae acquires resistance to 2-deoxyglucose at a very high frequency.
We have found that Saccharomyces cerevisiae acquires spontaneously increasing resistance to 2-deoxyglucose at a very high frequency. This finding allows the easy isolation of different types of resistant strains of interest for metabolic studies with 2-deoxyhexoses. On the other hand, it sounds a note of caution in the widespread use of 2-deoxyglucose as a selective agent for the isolation of yeast mutants with impaired hexose transport or phosphorylation systems.
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M F
MF
Instituto de Investigaciones Biomédicas, Facultad de Medicina, Universidad Autónoma, Spain.
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C F
CF
D003837:Deoxy Sugars / Q000494:pharmacology*; D003847:Deoxyglucose / Q000494:pharmacology*; D004352:Drug Resistance, Microbial; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics
D003837:Deoxy Sugars; D003847:Deoxyglucose