Network regulation in rheumatoid arthritis. Studies of DR+ T cells, anti-DR, antiidiotypic antibodies, and clinical disease activity.
We prospectively studied rheumatoid arthritis patients with various degrees of clinical disease activity, for the presence of DR+ T cells by flow cytometry, for anti-DR using immunoblot analysis, and for antiidiotypic (anti-id) antibodies by enzyme-linked immunosorbent assay using F(ab')2 monoclonal antibody anti-DR L243 as idiotype. DR+ T cells correlated positively with anti-DR, and anti-id correlated negatively with both DR+ T cells and anti-DR. Active clinical disease correlated positively with both DR+ T cells and anti-DR, and correlated negatively with anti-id. This DR antigen/anti-DR/anti-id network may control disease activity in rheumatoid arthritis patients.
Searles
R P
RP
Lovelace Medical Foundation, Division of Biomedical Research, Albuquerque, NM 87108.
Savage
S M
SM
Brozek
C M
CM
Marnell
L L
LL
Hoffman
C L
CL
D000906:Antibodies / Q000032:analysis*; D000888:Antibodies, Anti-Idiotypic / Q000032:analysis*; D000911:Antibodies, Monoclonal; D001172:Arthritis, Rheumatoid / Q000276:immunology* / Q000503:physiopathology; D004797:Enzyme-Linked Immunosorbent Assay; D006681:HLA-D Antigens / Q000032:analysis*; D006684:HLA-DR Antigens / Q000032:analysis* / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D007158:Immunologic Techniques; D013601:T-Lymphocytes / Q000276:immunology*
D000906:Antibodies; D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D006681:HLA-D Antigens; D006684:HLA-DR Antigens; D007074:Immunoglobulin G; D007130:Immunoglobulin Idiotypes