Characterisation of a monoclonal antibody capable of neutralising the haemorrhagic activity of West African Echis carinatus (carpet viper) venom.
A murine monoclonal antibody (SV-1/F10) was highly specific immunologically for West African E.carinatus venom both by ELISA and immunoblotting. In cross-protection tests in vivo, it possessed strong antihaemorrhagic activity. This IgGl monoclonal antibody recognised an epitope present in a protein band of 124,000 mol. wt using immunoblotting of non-reduced Nigerian and Ghanaian E.carinatus venoms, as well as in a second protein band of 105,000 mol. wt in Ghanaian E.carinatus venom. The SV-1/F10 monoclonal antibody is of potential use for the isolation of West African E.carinatus venom haemorrhagin from whole venom, allowing the possibility of elucidation of the mechanism of its actions as well as its interactions with antibody.
Iddon
D
D
Department of Tropical Medicine and Infectious Diseases, Liverpool School of Tropical Medicine, U.K.
Hommel
M
M
Theakston
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RD
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology / Q000302:isolation & purification*; D000997:Antivenins; D001777:Blood Coagulation / Q000187:drug effects; D002999:Clone Cells; D004591:Electrophoresis, Polyacrylamide Gel; D005340:Fibrinogen / Q000378:metabolism; D006470:Hemorrhage / Q000139:chemically induced; D006825:Hybridomas; D007074:Immunoglobulin G / Q000032:analysis; D007158:Immunologic Techniques; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008815:Mice, Inbred Strains; D009500:Neutralization Tests; D051381:Rats; D014757:Viper Venoms / Q000276:immunology* / Q000633:toxicity
D000911:Antibodies, Monoclonal; D000997:Antivenins; D007074:Immunoglobulin G; D014757:Viper Venoms; D005340:Fibrinogen