title: Oncogene expression in related cancer lines differing in metastatic capacity.
abstract: Various murine tumor cell lines with different metastatic capacities were tested in vitro for oncogene expression, especially of the p21-Ha-ras protein. Small differences were seen in the expression of several distinct oncogenes in the case of a high metastatic lymphoma variant (ESb) and its low metastatic parental line (Eb). In one instance we observed a 30-fold Ha-ras gene amplification in a metastasis-derived cell line from a spontaneous mouse mammary carcinoma. In spite of this amplification we did not find an increased p21 expression in these cells.
authors.affiliation: Institute for Immunology and Genetics, German Cancer Research Center, Heidelberg.
authors.forename: J
authors.initials: J
authors.lastname: Pohl
authors.forename: A
authors.initials: A
authors.lastname: Radler-Pohl
authors.forename: R
authors.initials: R
authors.lastname: Heicappell
authors.forename: V
authors.initials: V
authors.lastname: Schirrmacher
mesh_terms: D000818:Animals; D002460:Cell Line; D004273:DNA, Neoplasm / Q000032:analysis; D005784:Gene Amplification; D008325:Mammary Neoplasms, Experimental / Q000235:genetics* / Q000473:pathology; D051379:Mice; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D009362:Neoplasm Metastasis; D009857:Oncogenes; D011518:Proto-Oncogene Proteins / Q000032:analysis; D016283:Proto-Oncogene Proteins p21(ras); D012333:RNA, Messenger / Q000032:analysis
chemical_list: D004273:DNA, Neoplasm; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D016283:Proto-Oncogene Proteins p21(ras)