High-efficiency generation of plasmid cDNA libraries using electro-transformation.
A simple and efficient method for the construction of large cDNA libraries in plasmid vectors is described. Cloning efficiencies of greater than 1 x 10(6) recombinants per microgram starting RNA are easily obtained using electroporation. A detailed protocol for determining the optimal conditions using this novel approach is presented.
Böttger
E C
EC
Biogen Research Corporation.
D001483:Base Sequence; D002130:Calcium Phosphates; D011232:Chemical Precipitation; D004247:DNA / Q000235:genetics*; D004566:Electrodes; D015723:Gene Library; D006801:Humans; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics*; D014170:Transformation, Genetic
D002130:Calcium Phosphates; C485828:alpha-tricalcium phosphate; C485830:tetracalcium phosphate; C485838:calcium phosphate, monobasic, anhydrous; D004247:DNA; C020243:calcium phosphate; C485829:calcium phosphate, dibasic, anhydrous