Isolation of alligator gar (Lepisosteus spatula) glucagon, oxyntomodulin, and glucagon-like peptide: amino acid sequences of oxyntomodulin and glucagon-like peptide.
Oxyntomodulin, glucagon, and a glucagon-like peptide (GLP) have been isolated from the endocrine pancreas of the alligator gar (Lepisosteus spatula), a ganoid fish. The three peptides were isolated by gel filtration and HPLC and were identified by size, composition, and glucagon-like immunoreactivity. The amino acid sequences of the oxyntomodulin and GLP were determined. The oxyntomodulin contains 36 amino acid residues and its sequence is H S Q G T F T N D Y S K Y L D T R R A Q D F V Q W L M S T K R S G G I T. The composition of the glucagon is identical to the N-terminal 29 residues of the gar oxyntomodulin. The single form of GLP found contains 34 amino acid residues in the following sequence: H A D G T Y T S D V S S Y L Q D Q A A K K F V T W L K Q G Q D R R E. These findings suggest that all three peptides are derived from a common precursor.
Pollock
H G
HG
Department of Biochemistry, University of Kansas Medical Center, Kansas City 66103.
Kimmel
J R
JR
Ebner
K E
KE
Hamilton
J W
JW
Rouse
J B
JB
Lance
V
V
Rawitch
A B
AB
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D005260:Female; D005399:Fishes / Q000378:metabolism*; D005768:Gastrointestinal Hormones / Q000302:isolation & purification*; D005934:Glucagon / Q000302:isolation & purification*; D004763:Glucagon-Like Peptides / Q000302:isolation & purification*; D007515:Islets of Langerhans / Q000032:analysis*; D008297:Male; D008969:Molecular Sequence Data; D053772:Oxyntomodulin; D010455:Peptides / Q000302:isolation & purification*; D011863:Radioimmunoassay
D000596:Amino Acids; D005768:Gastrointestinal Hormones; D053772:Oxyntomodulin; D010455:Peptides; D004763:Glucagon-Like Peptides; C015236:glucagon-like-immunoreactivity; D005934:Glucagon