title: Separation and detection of DNA by capillary electrophoresis.
abstract: The use of capillary electrophoresis for the separation and detection of nucleic acids has been investigated. Lab-model instruments have been built, using commercially available UV absorbance and fluorescence detectors which were modified for use with 50-100 microns I.D. fused-silica capillary tubing. The sensitivity of these instruments (signal-to-noise ratio = 3) was measured as 15 micrograms/ml for fluorescence detection of ethidium bromide-stained herring-sperm DNA and 3 micrograms/ml for UV absorbance detection. With the former instruments a variety of strategies has been used to attain rapid separations of bases, oligonucleotides, restriction fragments and whole phage, viral and plasmid DNAs.
authors.affiliation: Microphoretic Systems, Inc., Sunnyvale, CA 94086.
authors.forename: T J
authors.initials: TJ
authors.lastname: Kasper
authors.forename: M
authors.initials: M
authors.lastname: Melera
authors.forename: P
authors.initials: P
authors.lastname: Gozel
authors.forename: R G
authors.initials: RG
authors.lastname: Brownlee
mesh_terms: D004247:DNA / Q000032:analysis*; D015252:Deoxyribonucleases, Type II Site-Specific; D004586:Electrophoresis; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
chemical_list: D004247:DNA; D015252:Deoxyribonucleases, Type II Site-Specific; C024403:GGCC-specific type II deoxyribonucleases