Genetic and phenotypic analyses indicating occurrence of the recN262 and radB101 mutations at the same locus in Escherichia coli.
The radB101 and recN262 mutations showed essentially identical phenotypes when compared in isogenic Escherichia coli strains for their effects on gamma and UV radiation survival and on conjugal recombination in a uvrA recB recC sbcB sbcC strain. Complementation tests involving attempts to reconstitute a radB+ recN+ strain by transductions between radB101 and recN262 donors and recipients, and tests involving plasmids carrying recN+ and recN::Tn1000 inserts, indicated that the radB and recN genes are identical. We suggest that the radB101 mutation now be referred to as recN2001.
Sargentini
N J
NJ
Department of Radiation Oncology, Stanford University School of Medicine, California 94305.
Smith
K C
KC
D001426:Bacterial Proteins / Q000235:genetics*; D002874:Chromosome Mapping; D003227:Conjugation, Genetic; D004262:DNA Restriction Enzymes; D004926:Escherichia coli / Q000235:genetics* / Q000528:radiation effects; D005720:Gamma Rays; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D009154:Mutation; D010641:Phenotype; D011995:Recombination, Genetic; D014161:Transduction, Genetic; D014169:Transformation, Bacterial; D014466:Ultraviolet Rays
D001426:Bacterial Proteins; D004262:DNA Restriction Enzymes; C047879:RecN protein, Bacteria