Immunohistochemical identification of Trichosporon beigelii in histologic section by immunoperoxidase method.
An indirect immunoperoxidase method capable of identifying an opportunistic fungus, Trichosporon beigelii, in formalin-fixed, paraffin-embedded tissue has been developed. The authors studied autopsy materials from 19 patients with various fungal infections, including 3 patients with disseminated T. beigelii infection, 8 patients with localized or systemic candidiasis, 4 patients with invasive aspergillosis, 2 patients with pulmonary mucormycosis, and 2 patients with systemic cryptococcosis. T. beigelii could be successfully visualized in tissue sections from all three patients with this infection and a patient with systemic candidiasis who was found to have concomitant infection by T. beigelii. The capsule of Cryptococcus neoformans was also stained, indicating the presence of cross-reactive antigen between T. beigelii and C. neoformans. C. neoformans-absorbed antiserum was reactive with T. beigelii but not with other fungi, including C. neoformans.
Kobayashi
M
M
Department of Medicine, Kochi Medical School, Japan.
Kotani
S
S
Fujishita
M
M
Taguchi
H
H
Moriki
T
T
Enzan
H
H
Miyoshi
I
I
D000908:Antibodies, Fungal / Q000276:immunology; D000918:Antibody Specificity; D002176:Candida albicans / Q000276:immunology / Q000302:isolation & purification; D005455:Fluorescent Antibody Technique; D006801:Humans; D007106:Immune Sera; D007108:Immune Tolerance; D007124:Immunoenzyme Techniques; D008168:Lung / Q000382:microbiology; D003904:Mitosporic Fungi / Q000302:isolation & purification*; D009181:Mycoses / Q000175:diagnosis / Q000382:microbiology / Q000473:pathology; D012189:Retrospective Studies; D014250:Trichosporon / Q000276:immunology / Q000302:isolation & purification*
D000908:Antibodies, Fungal; D007106:Immune Sera