title: Fluorescence-activated cell-sorting analysis of fibronectin peptides binding to Trypanosoma cruzi trypomastigotes.
abstract: The binding of synthetic peptides modeled from the sequence of the cell attachment site of fibronectin to T. cruzi trypomastigote surface receptors was investigated by fluorescence-activated cell-sorting analysis using fluorescein-labeled peptides. Peptides with the sequence Arg-Gly-Asp-Ser bound to the parasite surface. A low percentage of fresh parasites recently liberated from infected fibroblasts had the capacity to bind the peptide. In contrast, these parasites showed a time-dependent several-fold increase in their ability to bind the Arg-Gly-Asp-Ser-containing peptides during extracellular incubation. From these observations, it appears that the expression of surface receptors on a particular, mature stage of the parasite parallels its ability to adhere to and infect host cells.
authors.affiliation: Centre d'Immunologie et de Biologie Parasitaire, Unité Mixte INSERM 167--CNRS 624, Lille, France.
authors.forename: M A
authors.initials: MA
authors.lastname: Quaissi
authors.forename: J P
authors.initials: JP
authors.lastname: Kusnierz
authors.forename: H
authors.initials: H
authors.lastname: Gras-Masse
authors.forename: H
authors.initials: H
authors.lastname: Drobecq
authors.forename: P
authors.initials: P
authors.lastname: Velge
authors.forename: J
authors.initials: J
authors.lastname: Cornette
authors.forename: A
authors.initials: A
authors.lastname: Capron
authors.forename: A
authors.initials: A
authors.lastname: Tartar
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002469:Cell Separation; D005353:Fibronectins / Q000032:analysis* / Q000378:metabolism; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000032:analysis* / Q000378:metabolism; D014349:Trypanosoma cruzi / Q000032:analysis / Q000378:metabolism*
chemical_list: D005353:Fibronectins; D010446:Peptide Fragments