title: Reversion of Lactobacillus lactis protoplasts.
abstract: More than 99% of L. lactis cells have been converted to protoplasts upon digestion of cell walls with mutanolysin (N-(acetyl)muramidase). Functional protoplasts were obtained even with the lowest level of the enzyme that was used (0.1 U.ml-1 of the cell suspension) and after incubation at 37 degrees C for 2 min. The regeneration of the polymerized cell wall appears to be induced by a cell homogenate of the same organism.
authors.affiliation: Swiss Federal Dairy Research Institute, Liebefeld-Bern.
authors.forename: P
authors.initials: P
authors.lastname: Bican
authors.forename: A
authors.initials: A
authors.lastname: Spahni
mesh_terms: D002473:Cell Wall / Q000502:physiology; D010450:Endopeptidases / Q000378:metabolism; D007778:Lactobacillus / Q000502:physiology* / Q000648:ultrastructure; D008854:Microscopy, Electron; D011523:Protoplasts / Q000502:physiology*
chemical_list: D010450:Endopeptidases; C021816:mutanolysin