Insulin-like growth factors in the Göttinger miniature-pig.
IGF I was determined by a radioimmunoassay and IGF II by a radioreceptorassay in 20 Göttinger miniature (mini)-pigs and 13 domestic pigs of different weight and age. Immunoreactive IGF I serum levels of mini-pigs were similar to those of domestic pigs in corresponding age-classes (150-250 and 100-270 micrograms/l, respectively). No differences were detectable between receptor-reactive IGF II serum levels in mini-pigs (150-200 micrograms/l) and domestic pigs (110-270 micrograms/l) nor did the biological insulin-like activities (measured in the rat fat cell assay) differ in mini- and domestic pigs (81-100 and 71-98 mU insulin/l, respectively). IGF I and IGF II decreased drastically after hypophysectomy in one of the mini-pigs. Intravenous bolus injections of 30 micrograms/kg of recombinant human IGF I in 4 mini-pigs caused a similar degree of hypoglycemia (nadir of blood glucose 1.33 +/- 0.61 mmol/l) as 0.15 IU insulin/kg, followed by a sharp growth hormone peak. We conclude that the marked difference between mini- and domestic pigs regarding body size is unrelated to serum levels of IGF I and II, a lack of response of tissues to IGF I or a reduced growth hormone secretory capacity in the mini-pig.
Zenobi
P D
PD
Department of Medicine, University Hospital, Zürich, Switzerland.
Guler
H P
HP
Zapf
J
J
Froesch
E R
ER
D000818:Animals; D001681:Biological Assay; D005260:Female; D006207:Half-Life; D007003:Hypoglycemia; D007016:Hypophysectomy; D007328:Insulin / Q000097:blood; D007334:Insulin-Like Growth Factor I / Q000008:administration & dosage / Q000097:blood; D007335:Insulin-Like Growth Factor II / Q000097:blood; D013002:Somatomedins / Q000097:blood*; D013552:Swine; D013556:Swine, Miniature / Q000097:blood*
D007328:Insulin; D013002:Somatomedins; D007334:Insulin-Like Growth Factor I; D007335:Insulin-Like Growth Factor II