Cytokine activity after human bone marrow transplantation. II. Production of macrophage procoagulant activity and the cytokine regulating its production, macrophage procoagulant inducing factor.
The production of procoagulant activity by circulating monocytes and its regulation by a cytokine secreted by mitogen-stimulated peripheral blood mononuclear cells was investigated in recipients of HLA-identical sibling bone marrow transplants. Blood monocyte numbers reached the normal range within 3 weeks of transplant. After stimulation with lipopolysaccharide, macrophage procoagulant activity was found to be within the normal range in all patients at all times post transplant. It did not appear to correlate with the presence or absence of graft-versus-host disease. Surprisingly, and in marked contrast to our previously documented severe depression of interleukin 2 production by transplant recipients' peripheral blood mononuclear cells, the mitogen-induced production of the cytokine that induces procoagulant activity production (macrophage procoagulant inducing factor, MPIF) was also normal in the majority of patients when assayed using the responsive myelomonocytic cell line RC-2A. These findings suggest firstly that monocyte differentiation and function normalize rapidly post transplant; and secondly, when taken together with previous studies, that the ability of peripheral blood mononuclear cells to synthesize cytokines post transplant varies greatly according to the specific cytokine involved.
Bentel
J
J
Department of Haematology, St Vincent's Hospital, Sydney, N.S.W., Australia.
Atkinson
K
K
D000293:Adolescent; D000328:Adult; D001688:Biological Products / Q000378:metabolism*; D001779:Blood Coagulation Factors / Q000378:metabolism; D016026:Bone Marrow Transplantation; D002648:Child; D003208:Concanavalin A / Q000494:pharmacology; D016207:Cytokines; D005260:Female; D006801:Humans; D007958:Leukocyte Count; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009000:Monocytes; D011184:Postoperative Period; D013997:Time Factors
D001688:Biological Products; D001779:Blood Coagulation Factors; D016207:Cytokines; C045072:macrophage procoagulant activity; D003208:Concanavalin A