title: Histamine H1-receptor in endothelial and smooth muscle cells of guinea-pig aorta.
abstract: The location of histamine H1-receptors in the thoracic aorta of guinea-pigs was studied with a [3H]mepyramine binding assay. [3H]Mepyramine binding studies of whole and rubbed aortas, and of cultured endothelial and smooth muscle cells showed that the Kd values were all in the range 0.53-0.76 nM, but that the Bmax values were 19.1, 10.1, 63.3 and 11.6 fmol/mg protein, respectively. Thus, the whole aorta contained more H1-receptors than the rubbed one (free of endothelium), and cultured endothelial cells contained more H1-receptors than smooth muscle cells. These results indicate that more histamine H1-receptors were concentrated in the endothelial cells than in the smooth muscle cells in guinea-pig aorta.
authors.affiliation: Department of Dermatology, Hiroshima University School of Medicine, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Hide
authors.forename: H
authors.initials: H
authors.lastname: Fukui
authors.forename: T
authors.initials: T
authors.lastname: Watanabe
authors.forename: H
authors.initials: H
authors.lastname: Wada
authors.forename: S
authors.initials: S
authors.lastname: Yamamoto
mesh_terms: D000818:Animals; D001013:Aorta, Thoracic / Q000032:analysis*; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000032:analysis* / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D006168:Guinea Pigs; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000032:analysis* / Q000648:ultrastructure; D011738:Pyrilamine / Q000378:metabolism; D011968:Receptors, Histamine / Q000032:analysis*; D011969:Receptors, Histamine H1 / Q000032:analysis*; D014316:Tritium
chemical_list: D011968:Receptors, Histamine; D011969:Receptors, Histamine H1; D014316:Tritium; D011738:Pyrilamine