In vivo localisation of radiolabelled monoclonal antibody in human gliomas.
F(ab')2-fragments of the anti-melanoma monoclonal antibody MeI-14 were labelled with 123I for external scanning and with 125I for tissue measurement of radioactivity and injected intravenously into patients scheduled for surgical resection of a glioma. The paired-label study was performed by injecting simultaneously 131I-labelled control (F(ab')2-fragments. The patients were scanned by computerised tomoscintigraphy. After surgery, the activities of 125I and 131I were counted in tumour and normal tissues. The results indicate that there was a low but definite uptake of the antibody in the tumour due to its specificity. The external detection was difficult because of accumulation of antibody fragments in the skull.
Behnke
J
J
Neurosurgical Service, CHUV, Lausanne, Switzerland.
Mach
J P
JP
Buchegger
F
F
Carrel
S
S
Delaloye
B
B
De Tribolet
N
N
D000911:Antibodies, Monoclonal / Q000493:pharmacokinetics*; D001932:Brain Neoplasms / Q000000981:diagnostic imaging / Q000276:immunology*; D005910:Glioma / Q000000981:diagnostic imaging / Q000276:immunology*; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000493:pharmacokinetics*; D014055:Tomography, Emission-Computed / Q000379:methods*
D000911:Antibodies, Monoclonal; D007140:Immunoglobulin Fab Fragments