title: Effect of lead acetate on Sertoli cell lactate production and protein synthesis in vitro.
abstract: The effects of lead acetate on protein synthesis and lactate production by cultures of rat Sertoli cells in vitro were studied. Sertoli cell cultures prepared from 20 day old Sprague-Dawley rats were exposed to 0.01, 0.05 and 0.10 mM lead acetate. Lactate production was significantly elevated by all concentrations of lead after 3, 6, 9 and 12 hours of exposure. Protein biosynthesis as measured by [3H]-leucine incorporation was significantly depressed by 0.05 and 0.10 mM lead acetate after 2 hours of exposure. These results support the hypothesis that lead acetate may inhibit spermatogenesis by a disturbance of the metabolic activities of the Sertoli cells.
authors.affiliation: Department of Environmental and Industrial Health, School of Public Health, University of Michigan, Ann Arbor.
authors.forename: L I
authors.initials: LI
authors.lastname: Batarseh
authors.forename: M J
authors.initials: MJ
authors.lastname: Welsh
authors.forename: M J
authors.initials: MJ
authors.lastname: Brabec
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D007773:Lactates / Q000378:metabolism*; D019344:Lactic Acid; D008297:Male; D009942:Organometallic Compounds / Q000633:toxicity*; D014176:Protein Biosynthesis; D051381:Rats; D011919:Rats, Inbred Strains; D012708:Sertoli Cells / Q000378:metabolism*; D013089:Spermatocidal Agents / Q000633:toxicity*
chemical_list: D007773:Lactates; D009942:Organometallic Compounds; D013089:Spermatocidal Agents; D019344:Lactic Acid; C008261:lead acetate