Alpha-factor-directed synthesis of Bacillus stearothermophilus alpha-amylase in Saccharomyces cerevisiae.
Promoter and leader sequence of Bacillus stearothermophilus alpha-amylase gene were removed and the gene was joined in-frame to sequences encoding the leader region of Saccharomyces cerevisiae mating pheromone alpha-factor on plasmid p69A (a hybrid of pBR322 and S. cerevisiae 2-microns plasmid). S. cerevisiae cells were transformed with plasmids containing the hybrid genes, obtaining yeast transformants which exhibit a significant extra-cellular amylolytic activity in solid medium, but not in liquid medium. Levels of alpha-amylase activity in solid medium were found to depend on the mode of fusion of the alpha-amylase gene to the alpha-factor leader region.
Nonato
R V
RV
Department of Life Science, Faculty of Science, Tokyo Institute of Technology, Yokohama, Japan.
Shishido
K
K
D000595:Amino Acid Sequence; D001483:Base Sequence; D005796:Genes; D005798:Genes, Bacterial; D005800:Genes, Fungal; D001411:Geobacillus stearothermophilus / Q000201:enzymology / Q000235:genetics*; D000072235:Mating Factor; D008969:Molecular Sequence Data; D010455:Peptides / Q000235:genetics*; D010675:Pheromones / Q000235:genetics*; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D000516:alpha-Amylases / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism
D010455:Peptides; D010675:Pheromones; D000072235:Mating Factor; D000516:alpha-Amylases