Loss of polymorphic A and B locus HLA antigens in colon carcinoma.
In the present study we have confirmed that approximately one third of human colorectal carcinomas fail to express the HLA - A,B,C monomorphic determinant reactive with the W6/32 MAb, and 44% express class II HLA antigens as shown by reactivity with NFK-1 MAb. Reduced staining with the W6/32 MAb was not always associated with the loss of beta 2m. In addition, the expression of HLA-A2 and Bw4 class I specific haplotypes on normal colon epithelium and tumour biopsy tissue was assessed. All normal colonic epithelia stained positively with MAb against A2 and Bw4 antigens, but a loss of these determinants was shown on tumour biopsies from patients tissue typed for the respective specificities. Loss of the A2 haplotype was shown in 4 of 15 tumour tissue samples, and loss of Bw4 specificities in 5 out of 7 tissue samples. The failure to detect specific loci determinants was not necessarily associated with loss of reactivity with W6/32 MAb.
Rees
R C
RC
Department of Virology, University of Sheffield Medical School, UK.
Buckle
A M
AM
Gelsthorpe
K
K
James
V
V
Potter
C W
CW
Rogers
K
K
Jacob
G
G
D000230:Adenocarcinoma / Q000276:immunology; D003110:Colonic Neoplasms / Q000276:immunology*; D006680:HLA Antigens / Q000032:analysis*; D015789:HLA-A2 Antigen; D015235:HLA-B Antigens; D006801:Humans; D007413:Intestinal Mucosa / Q000276:immunology; D012004:Rectal Neoplasms / Q000276:immunology; D001613:beta 2-Microglobulin / Q000032:analysis
D006680:HLA Antigens; D015789:HLA-A2 Antigen; D015235:HLA-B Antigens; C042388:HLA-Bw4 antigen; D001613:beta 2-Microglobulin