Quantitative determination of human protein C. Evaluation of a "fast" functional assay in comparison to a "traditional" functional and an immunological assay.
A fast functional assay for protein C was evaluated and compared with a traditional functional and an enzyme linked immunosorbent assay in parallel for the same plasma samples derived from 43 healthy subjects, 12 patients with severe hepatic dysfunction, and 23 patients under stable oral anticoagulation. By all three test systems significantly lower levels of protein C were obtained in both groups of patients compared with normal subjects (p less than 0.0001). No significant between - assay differences were found in normal subjects and in patients with hepatic dysfunction; by correlation analysis coefficients higher than 0.8 were calculated between the measurements of the three tests. In patients under stable oral anticoagulation, however, the immunologic test yielded higher values than the traditional (p less than 0.05) and, more pronounced, the fast functional assay (p less than 0.0001); no or only borderline significant correlations between the results were found. In these patients protein C levels measured with the traditional functional assay were in the same range as the activity levels of factors II, VII, IX, and X, whereas the fast functional test yielded significantly lower levels. The presented results indicate that very similar protein C levels were obtained with both functional and the immunologic assay except in patients under oral anticoagulation.
Vukovich
T C
TC
Institute of Medical Physiology, University of Vienna, Austria.
Knöbl
P N
PN
Bauer
K
K
Tragl
K H
KH
Deutsch
E
E
D000328:Adult; D000925:Anticoagulants; D001774:Blood Chemical Analysis / Q000379:methods* / Q000592:standards; D001779:Blood Coagulation Factors / Q000032:analysis; D005260:Female; D006801:Humans; D007158:Immunologic Techniques; D008107:Liver Diseases / Q000097:blood; D008297:Male; D008875:Middle Aged; D011486:Protein C / Q000097:blood*; D012016:Reference Values; D013997:Time Factors; D014812:Vitamin K / Q000494:pharmacology
D000925:Anticoagulants; D001779:Blood Coagulation Factors; D011486:Protein C; D014812:Vitamin K