Control of angiotensinogen production by H4 rat hepatoma cells in serum-free culture.
A serum-free, hormone and attachment factor supplemented culture for rat H4 hepatoma cells was established. In the defined medium (Dulbecco's Modified Eagle's + Ham's F12 + insulin, transferrin, fibronectin liver cell growth factor, and sodium selenite), H4 cells grew equally well as in 10% fetal bovine serum supplemented medium. H4 cells in either defined or serum-containing culture conditions produce transferrin but not albumin or alpha-fetoprotein. In this paper we have studied the effect of various hormones and pressor peptides on the production of angiotensinogen by H4 cells cultured in defined conditions. Only glucocorticoid hormone had a significant effect on the production of angiotensinogen, whereas other hormones previously reported to exert their effect on angiotensinogen production had little or no effect.
Togami
M
M
Japan Synthetic Rubber Co., Ltd., Kanagawa, Japan.
Blazka
D
D
Hayashi
J
J
D000803:Angiotensin I / Q000378:metabolism; D000808:Angiotensinogen / Q000096:biosynthesis*; D000818:Animals; D001769:Blood; D002455:Cell Division / Q000187:drug effects; D003470:Culture Media; D003907:Dexamethasone / Q000494:pharmacology; D005353:Fibronectins / Q000494:pharmacology; D005938:Glucocorticoids / Q000494:pharmacology*; D006133:Growth Substances / Q000494:pharmacology; D006854:Hydrocortisone / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology; D008114:Liver Neoplasms, Experimental / Q000378:metabolism*; D051381:Rats; D020887:Selenious Acid; D012643:Selenium / Q000494:pharmacology; D014168:Transferrin / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D003470:Culture Media; D005353:Fibronectins; D005938:Glucocorticoids; D006133:Growth Substances; D007328:Insulin; D014168:Transferrin; D000808:Angiotensinogen; D003907:Dexamethasone; D000803:Angiotensin I; D020887:Selenious Acid; D012643:Selenium; D006854:Hydrocortisone