[Isolation of a cellulase hyperproducer Trichoderma pseudokoningii mutant].
The present study describes the production of cellulases by a native strain of Trichoderma pseudokoningii in the presence and absence of a catabolic repressor. We report a plate assay for selecting fungal mutants capable of synthesizing the cellulase enzymes under conditions of catabolite repression. This method was used for the selection of catabolite repression-resistant mutants. One mutant, B-1, secreted 3.3 times the extracellular protein and 3 times the endoglucanase and filter paper activity in submerged cultures when compared with the wild type strain. The cellobiase activity remained at similar levels in both strains. Although this mutant was isolated as i cellulase producer in the presence of 500 mM glycerol on agar plates, in liquid medium containing the repressor B-1 it exhibits only partial derepression of the cellulase complex.
Zaldívar
M
M
Departamento de Bioquímica y Biología Molecular, Facultad de Ciencas Químicas y Farmacéuticas, Universidad de Chile, Santiago.
Steiner
J
J
Musalem
M
M
Contreras
I
I
D002475:Cellobiose / Q000378:metabolism; D002480:Cellulase / Q000096:biosynthesis* / Q000378:metabolism; D003470:Culture Media; D006026:Glycoside Hydrolases / Q000096:biosynthesis* / Q000378:metabolism; D003904:Mitosporic Fungi / Q000302:isolation & purification*; D009154:Mutation; D014242:Trichoderma / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification*
D003470:Culture Media; D002475:Cellobiose; D006026:Glycoside Hydrolases; D002480:Cellulase; C019826:carboxymethylcellulase
Aislamiento de un mutante de Trichoderma pseudokoningii hiperproductor de celulasas.