title: A subpopulation of cultured avian neural crest cells has transient neurogenic potential.
abstract: Neural crest cells of vertebrate embryos produce neurons, glia, pigment cells, and connective tissue in vivo and in vitro. To test the developmental potential of apparently undifferentiated crest cells, we have used the monoclonal antibody A2B5, which recognizes a cell surface glycolipid characteristic of neurons, to identify and immunoablate a subpopulation of cultured avian neural crest cells with a neuronal phenotype. Our results indicate that a limited neurogenic precursor subpopulation is present in cultures of avian neural crest cells and that the fate of this subpopulation can be influenced by environmental conditions arising when dispersal of neural crest cells is delayed.
authors.affiliation: Institute of Neuroscience, University of Oregon, Eugene 97403.
authors.forename: K S
authors.initials: KS
authors.lastname: Vogel
authors.forename: J A
authors.initials: JA
authors.lastname: Weston
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D002454:Cell Differentiation; D002455:Cell Division; D002478:Cells, Cultured; D005314:Embryonic and Fetal Development; D005732:Gangliosides / Q000276:immunology*; D009432:Neural Crest / Q000166:cytology* / Q000276:immunology / Q000502:physiology; D009473:Neuronal Plasticity; D011784:Quail / Q000196:embryology*; D013936:Thymidine; D013997:Time Factors
chemical_list: D000911:Antibodies, Monoclonal; D005732:Gangliosides; D013936:Thymidine