title: A stereochemical model of the transpeptidation complex.
abstract: Molecular models are proposed to describe the relative arrangement of aminoacyl and peptidyl tRNAs when bound to their respective A and P sites on the ribosome. The crystallographically determined structures of tRNAasp and tRNAphe have served as the models for these bound structures, while the imposed steric constraints for the model complexes were based on the results of published experimental data. The constructed models satisfy the stereochemical requirements needed for codon-anticodon interaction and for peptide bond formation. In this paper, the results of the complex containing tRNAphe as the A and P site bound transfer RNAs, is compared to a similarly constructed model which uses tRNAasp as the ribosome-bound transfer RNAs. The models have the following three major features: 1) the aminoacyl and peptidyl transfer RNAs assume an angle of approximately 45 degrees relative to each other; 2) in providing the proper stereochemistry for peptide bond condensation, a significant kink must be present in the messenger RNA between the A site and P site codons; and 3) a comparison of the two model complexes indicates that structural variations between the tRNAs or any allosteric transitions of the transfer RNAs associated with codon-anticodon recognition may be accommodated in the model by way of freedom of rotation about the phosphate backbone bonds in the mRNA between consecutive codons.
authors.affiliation: Department of Biophysics, Roswell Park Memorial Institute, Buffalo, New York 14263.
authors.forename: J J
authors.initials: JJ
authors.lastname: McDonald
authors.forename: R
authors.initials: R
authors.lastname: Rein
mesh_terms: D000926:Anticodon / Q000378:metabolism; D001665:Binding Sites; D003062:Codon / Q000378:metabolism; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D010441:Peptide Chain Elongation, Translational; D012333:RNA, Messenger / Q000648:ultrastructure*; D012346:RNA, Transfer, Amino Acyl / Q000648:ultrastructure*; D012270:Ribosomes / Q000648:ultrastructure*
chemical_list: D000926:Anticodon; D003062:Codon; D012333:RNA, Messenger; D012346:RNA, Transfer, Amino Acyl; C019037:tRNA, peptidyl-