Is MYB amplified in acute myelogenous leukemia-derived cell lines?
Amplification of MYB DNA has been reported in two cell lines, ML-1 and ML-3, derived from a patient with acute myelogenous leukemia. A relationship between amplification and a terminal deletion on one of the homologs of chromosome 6 has been proposed. The present study shows that equivalent copy numbers for MYB DNA are present on each of the homologs of chromosome 6. Further, no evidence for gene amplification could be demonstrated in either cell line.
Henderson
A S
AS
Department of Biological Sciences, Hunter College-CUNY, NY 10021.
Wolman
S R
SR
D000782:Aneuploidy; D002872:Chromosome Deletion; D002896:Chromosomes, Human, Pair 6; D004273:DNA, Neoplasm / Q000032:analysis; D005784:Gene Amplification; D006801:Humans; D007951:Leukemia, Myeloid / Q000235:genetics / Q000473:pathology*; D009857:Oncogenes; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D020598:Proto-Oncogene Proteins c-myb; D014407:Tumor Cells, Cultured / Q000032:analysis
D004273:DNA, Neoplasm; D011518:Proto-Oncogene Proteins; D020598:Proto-Oncogene Proteins c-myb