title: Colicin E8, a DNase which indicates an evolutionary relationship between colicins E2 and E3.
abstract: Colicin E8-J and its immunity protein were characterized with regard to their activities and gene structures. Colicin E8 is a complex of proteins A and B; protein A (the naked E8) exhibits an apparently nonspecific DNase activity that is inhibited by protein B (the immunity protein), as in the case of colicin E2. The nucleotide sequence of the downstream half of the colicin operon of ColE8-J was determined to be highly homologous to that of ColE2-P9, with the exception of the hot spot region of the 3'-terminal segment of the colicin gene and the adjacent immunity gene. The immE2-like gene of ColE3-CA38 was, as assumed previously, extensively homologous to the immE8 gene of ColE8-J, and thus, ColE8-J was shown to be situated between ColE2-P9 and ColE3-CA38 in the evolution of the E-group Col plasmids.
authors.affiliation: Department of Agricultural Chemistry, University of Tokyo, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Toba
authors.forename: H
authors.initials: H
authors.lastname: Masaki
authors.forename: T
authors.initials: T
authors.lastname: Ohta
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics / Q000302:isolation & purification; D003086:Bacteriocin Plasmids; D001483:Base Sequence; D005075:Biological Evolution; D003087:Colicins / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D003851:Deoxyribonucleases / Q000235:genetics* / Q000302:isolation & purification; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009876:Operon; D010957:Plasmids; D012689:Sequence Homology, Nucleic Acid
chemical_list: D001426:Bacterial Proteins; D003087:Colicins; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; C488855:ceiC protein, E coli; C007104:colicin immunity proteins; C488858:immE8 protein, E coli; D003851:Deoxyribonucleases