Different modulating effects of the monoamines adrenaline, noradrenaline, and serotonin on the DNA synthesis response of human peripheral blood T lymphocytes activated by mercuric chloride and nickel sulfate.
The influence of adrenaline, noradrenaline, and serotonin (5-hydroxytryptamine) was tested on the proliferative response of peripheral blood T lymphocytes of nickel-allergic subjects to mercuric chloride and nickel sulfate. With addition of the monoamines 1 h after mercuric chloride, there was a marked suppression with all the substances at 10(-4) M, while adrenaline also inhibited down to a concentration of 10(-5) M and serotonin down to 10(-7) M. At 3 days after mercuric chloride, adrenaline inhibited at 10(-4) M and serotonin also down to a concentration of 10(-5) M. With addition at 1 h after nickel sulfate, there was an inhibition by all the monoamines at 10(-4) M, while, in contrast, adrenaline at 10(-6) to 10(-8) M and serotonin at 10(-5) to 10(-6) M gave a stimulation of the response. These findings point at different modulating effects on the proliferative response induced by a polyclonal activator such as mercuric chloride and a monoclonal activator such as nickel sulfate.
Nordlind
K
K
Department of Dermatology, Karolinska Hospital, Stockholm, Sweden.
Sundström
E
E
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D015306:Biogenic Monoamines / Q000494:pharmacology*; D004247:DNA / Q000096:biosynthesis*; D004837:Epinephrine / Q000494:pharmacology; D006801:Humans; D007166:Immunosuppressive Agents / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects; D008627:Mercuric Chloride / Q000494:pharmacology*; D009532:Nickel / Q000494:pharmacology*; D012701:Serotonin / Q000494:pharmacology; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*
D000276:Adjuvants, Immunologic; D015306:Biogenic Monoamines; D007166:Immunosuppressive Agents; D012701:Serotonin; C029938:nickel sulfate; D008627:Mercuric Chloride; D009532:Nickel; D004247:DNA; D004837:Epinephrine