A replication region of the IncHI plasmid, R27, is highly homologous with the RepFIA replicon of F.
A region of the IncHI plasmid R27 has been found to share very close nucleotide sequence homology with the RepFIA replicon of F. This region has been located on a 1.6 kb segment of R27 plasmid DNA, and corresponds to ori-2 and the E gene of F. The incC repeat sequence region shows reduced homology, with the F repeats being an imperfect subset of a larger repeated sequence found in R27. The E gene homologue of R27 is able to initiate replication from the F ori-2 sequence and to repress the E gene promoter of F. The results are consistent with the observed incompatibility behaviour of R27, and have a bearing on the specificity of interaction of E protein with its DNA-binding sites.
Saul
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Department of Cell Biology, University of Auckland, New Zealand.
Lane
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D
Bergquist
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PL
D000595:Amino Acid Sequence; D001483:Base Sequence; D004261:DNA Replication; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D010957:Plasmids; D012093:Replicon; D012689:Sequence Homology, Nucleic Acid