Inhibition of anti-hapten antibody plaque-forming cells by a chemically reactive hapten.
The ability of the chemically reactive hapten 2-4-6-trinitrobenzene sulphonic acid (TNBSA) to inhibit anti-TNP IgM antibody plaque-forming cells (APFC) was investigated. TNBSA at a concentration of 1 mg (3 X 10(-3)M) to 0.3 mg (10(-3] completely inhibited IgM anti-TNP APFC after a 10-min incubation period at 37 degrees C. This inhibition was specific and did not alter the antifluorescein IgM antibody response. After a 24-h culture period in tissue culture medium without antigen, the non-dividing APFC remained inhibited, whereas the dividing APFC escaped this inhibition.
Huchet
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INSERM, Unité 267, Hôpital Paul-Brousse, Villejuif, France.
Grandjon
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D
D000818:Animals; D000917:Antibody Formation; D004306:Dose-Response Relationship, Immunologic; D006241:Haptens / Q000276:immunology / Q000494:pharmacology*; D006462:Hemolytic Plaque Technique; D007075:Immunoglobulin M / Q000276:immunology; D051379:Mice; D008811:Mice, Inbred DBA; D009578:Nitrobenzenes / Q000276:immunology*; D013997:Time Factors; D014301:Trinitrobenzenes / Q000276:immunology*
D006241:Haptens; D007075:Immunoglobulin M; D009578:Nitrobenzenes; D014301:Trinitrobenzenes