Genetic analysis of tumorigenesis. XXXI: Retention of short arm of chromosome 3 in suppressed CHEF cell hybrids containing c-Ha-ras (EJ) gene.
Hybrids between nontransformed Chinese hamster embryo fibroblast (CHEF) cells and their c-Ha-ras (EJ) -transformed derivatives are suppressed for tumor-forming ability when tested at early passage. Hybrid subclones with suppressed (fibroblastic) or transformed appearance have now been selected by multiple recloning. Morphology, but not serum or anchorage requirement, was a sensitive indicator of suppression: Subclones with normal morphology were nontumorigenic, subclones with transformed morphology were highly tumorigenic, and intermediate subclones (7-70% normal colonies) formed tumors with a frequency of 17-50%. Suppressed lines retained the short arm of chromosome 3, but transformed and tumor-derived lines had lost this region (greater than or equal to 1 copy). Transformed and tumor-derived cells exhibited additional chromosome changes, including the loss of at least one copy of chromosomes 7 and/or 8. These findings suggest that a tumor suppressor gene lies on the short arm of chromosome 3, consistent with prior studies from this laboratory. Other suppressor genes may be located on chromosomes 7 and 8.
Craig
R W
RW
Dana-Farber Cancer Institute, Boston, Massachusetts.
Gadi
I K
IK
Sager
R
R
D000818:Animals; D002471:Cell Transformation, Neoplastic; D002869:Chromosome Aberrations; D002871:Chromosome Banding; D006224:Cricetinae; D003412:Cricetulus; D011905:Genes, ras; D005819:Genetic Markers; D007621:Karyotyping; D011519:Proto-Oncogenes
D005819:Genetic Markers