The ras-related ral gene maps to chromosome 7p15-22.
Human cDNAs coding for the new protein ral that shares 50% homology with the ras proteins have been recently isolated. A 600-bp fragment carrying mainly the coding region was used to localize the ral gene by hybridization with sorted chromosomes and in situ hybridization. Direct molecular hybridization on sorted chromosomes using a single laser illumination allowed the assignment of the ral gene to a region of the flow karyotype containing chromosomes 7, 8 and X. With dual laser analysis ral was assigned to the fraction containing chromosome 7. In the 331 human metaphases hybridized with the 3H-labelled insert, the silver grain distribution showed a unique major signal on chromosome 7p15-22.
Rousseau-Merck
M F
MF
Unité INSERM U 301, CNRS, Paris, France.
Bernheim
A
A
Chardin
P
P
Miglierina
R
R
Tavitian
A
A
Berger
R
R
D002461:Cell Line, Transformed; D002874:Chromosome Mapping; D002897:Chromosomes, Human, Pair 7; D004247:DNA / Q000235:genetics; D005434:Flow Cytometry; D011905:Genes, ras; D006801:Humans; D007621:Karyotyping; D009693:Nucleic Acid Hybridization; D011518:Proto-Oncogene Proteins / Q000235:genetics; D016283:Proto-Oncogene Proteins p21(ras)
D011518:Proto-Oncogene Proteins; D004247:DNA; C501009:HRAS protein, human; D016283:Proto-Oncogene Proteins p21(ras)