Immunofluorescent localization of histone H10 in the nuclei of proliferating and differentiating Friend cells.
By using affinity-purified antibodies to H10 and to H1AB the localization of these histones was studied by indirect immunofluorescence in the nuclei of proliferating (EAT and uninduced Friend cells) and of differentiating (induced Friend cells) cell populations. While with H1AB antibodies a bright fluorescence all over the chromatin was obtained, the localization of H10 varied depending on the state of the cell population. In the proliferating EAT cells it was localized strictly in the nucleoli. The Friend cell population revealed a heterogeneous picture with two types of H10 localization-nucleolar predominating in uninduced cell populations and peripheral predominating in induced cells. A comparison with literature data suggests that H10 seems to be associated with chromatin regions containing active genes.
Banchev
T
T
Institute of Molecular Biology, Bulgarian Academy of Sciences, Sofia.
Srebreva
L
L
Zlatanova
J
J
Tsanev
R
R
D000818:Animals; D000906:Antibodies / Q000276:immunology; D000918:Antibody Specificity; D002454:Cell Differentiation; D002455:Cell Division; D002466:Cell Nucleolus / Q000032:analysis; D002467:Cell Nucleus / Q000032:analysis; D002843:Chromatin / Q000032:analysis; D005455:Fluorescent Antibody Technique; D005622:Friend murine leukemia virus; D006657:Histones / Q000032:analysis* / Q000276:immunology; D004915:Leukemia, Erythroblastic, Acute / Q000032:analysis* / Q000473:pathology; D051379:Mice
D000906:Antibodies; D002843:Chromatin; D006657:Histones