A gas chromatographic/mass spectrometric assay for catechol estrogens in microsomal incubations: comparison with a radiometric assay.
A gas chromatographic/mass spectrometric assay for quantifying two catechol estrogens, 2-hydroxyestradiol and 4-hydroxyestradiol, in microsomal preparations is described. The assay employs deuterium-labeled analogs of the catechol estrogens as internal standards and permits quantification of catechol estrogens, in microsomal incubations, at low (1-2) microM concentrations. The compounds are analyzed as their trimethylsilyl derivatives following separation by capillary gas chromatography.
Porubek
D J
DJ
Department of Medicinal Chemistry, School of Pharmacy, University of Washington, Seattle 98195.
Nelson
S D
SD
D000818:Animals; D003903:Deuterium; D004958:Estradiol / Q000031:analogs & derivatives* / Q000032:analysis; D002393:Estrogens, Catechol; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism*; D011865:Radioisotope Dilution Technique; D051381:Rats; D011919:Rats, Inbred Strains
D002393:Estrogens, Catechol; D004958:Estradiol; D003903:Deuterium; C001390:2-hydroxyestradiol; C014036:4-hydroxyestradiol