title: Changes in aerobic microflora of skin and gills of Mediterranean sardines (Sardina pilchardus) during storage in ice.
abstract: Sardines from the Adriatic Sea were examined fresh and after 4 and 8 days of storage in ice. A total of 1500 strains isolated were identified from the gills and the surface of the fish. Pseudomonadaceae, Neisseriaceae, Flavobacterium/Cytophaga, Enterobacteriaceae, coryneform bacteria and Micrococcaceae were the most common bacteria in fresh fish. During storage the pseudomonads (mainly the non-fluorescent strains) increased and became the dominating microflora; the Neisseriaceae (Moraxella, Psychrobacter and Acinetobacter) showed a distinct increase during the first 4 days in ice; the percentage of the other bacterial groups clearly decreased. On the gills the quantitative changes in the microflora were less pronounced than on the surface.
authors.affiliation: Istituto di Ispezione degli Alimenti di Origine Animale, cattedra I, Facoltà di Medicina Veterinaria, Università degli Studi di Milano, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Gennari
authors.forename: S
authors.initials: S
authors.lastname: Tomaselli
mesh_terms: D000818:Animals; D001419:Bacteria / Q000302:isolation & purification*; D015169:Colony Count, Microbial; D005399:Fishes / Q000382:microbiology*; D005516:Food Microbiology; D005519:Food Preservation; D005880:Gills / Q000382:microbiology*; D007053:Ice; D008522:Mediterranean Sea; D012867:Skin / Q000382:microbiology*
chemical_list: D007053:Ice