The radiation inactivation method provides evidence that membrane-bound mitochondrial creatine kinase is an oligomer.
Lyophilized suspensions of rabbit heart mitochondria have been irradiated with varying doses of gamma rays. Mitochondrial creatine kinase activity was inactivated exponentially with a radiation inactivation size of 352 or 377 kDa depending upon the initial medium. These values are in good agreement with the molecular mass previously deduced from by permeation experiments: 357 kDa. This is the first direct evidence showing that the native form of mitochondrial creatine kinase is associated to the inner membrane as an oligomer, very likely an octamer.
Quemeneur
E
E
LBTM-CNRS, Université Claude Bernard, Villeurbanne.
Eichenberger
D
D
Goldschmidt
D
D
Vial
C
C
Beauregard
G
G
Potier
M
M
D000818:Animals; D003402:Creatine Kinase / Q000032:analysis*; D004588:Electrophoresis, Cellulose Acetate; D007527:Isoenzymes; D046911:Macromolecular Substances; D008929:Mitochondria, Heart / Q000201:enzymology*; D011817:Rabbits; D011865:Radioisotope Dilution Technique
D007527:Isoenzymes; D046911:Macromolecular Substances; D003402:Creatine Kinase