Effects of t-butyl-4-hydroxyanisole and other phenolic antioxidants on tumoral cells and Trypanosoma parasites.
The antioxidant food additives 2(3)-tert-butyl-4-hydroxyanisole (BHA), 2,6-di(tert-butyl)-p-cresol (BHT) and the methyl and propyl esters of gallic acid inhibited Trypanosoma cruzi culture growth and oxygen consumption. The I50 values for growth and oxygen uptake with BHA were 0.284 and 0.400 and for BHT 0.083 and 0.235 mM, respectively. Moreover, BHA inhibited the respiration of several tumor cells, as well as of the procyclic and bloodstream trypomastigote forms of T. brucei brucei, with I50 in the range 0.29-0.52 mM. Inhibition of the parasites' oxygen uptake by BHA was not of the pure Michaelis-Menten type, but may be of a mixed form. It is postulated that these compounds are inhibitors because they resemble ubiquinone.
Ferreira
J
J
Department of Biochemistry and Chemistry, Faculty of Medicine, University of Chile, Santiago.
Coloma
L
L
Fones
E
E
Letelier
M E
ME
Repetto
Y
Y
Morello
A
A
Aldunate
J
J
D000818:Animals; D000970:Antineoplastic Agents; D000975:Antioxidants / Q000494:pharmacology* / Q000627:therapeutic use; D000981:Antiprotozoal Agents; D002083:Butylated Hydroxyanisole / Q000494:pharmacology* / Q000627:therapeutic use; D002286:Carcinoma, Ehrlich Tumor / Q000188:drug therapy; D002460:Cell Line; D051379:Mice; D009374:Neoplasms, Experimental / Q000188:drug therapy*; D010101:Oxygen Consumption / Q000187:drug effects; D010636:Phenols / Q000494:pharmacology* / Q000627:therapeutic use; D013045:Species Specificity; D014346:Trypanosoma brucei brucei / Q000187:drug effects* / Q000378:metabolism; D014349:Trypanosoma cruzi / Q000187:drug effects* / Q000378:metabolism
D000970:Antineoplastic Agents; D000975:Antioxidants; D000981:Antiprotozoal Agents; D010636:Phenols; D002083:Butylated Hydroxyanisole