Two-site immunoenzymometric assays for serum IgG subclass infant/maternal ratios at full-term.
Improvements were made in the range, precision, convenience, automation, and reliability of our previously published two-site immunoenzymometric assays using mouse monoclonal antibodies specific for human IgG and its subclasses. The serum concentration ratios for these immunoglobulin isotypes were measured in neonatal/maternal paired sera from 119 normal full-term deliveries. These ratios are significantly different than 1.0 (P = 0.001) for total IgG, IgG1, and IgG2 (show non-equality of paired neonatal/maternal sera concentrations) but are not significantly different than 1.0 for IgG3 and IgG4. On average, IgG1 is elevated 61%, and IgG2 is depressed 11% in the full-term neonate with respect to its own mother. In some pregnancies, active transport of IgG1 may be selectively enhanced by low material IgG1 concentration and selectively inhibited by high levels.
Black
C M
CM
Division of Host Factors, Centers for Disease Control, Atlanta, GA 30333.
Plikaytis
B D
BD
Wells
T W
TW
Ramirez
R M
RM
Carlone
G M
GM
Chilmonczyk
B A
BA
Reimer
C B
CB
D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D005312:Fetal Blood / Q000032:analysis*; D006801:Humans; D007124:Immunoenzyme Techniques / Q000592:standards; D007126:Immunoglobulin Allotypes / Q000032:analysis*; D007074:Immunoglobulin G / Q000032:analysis / Q000145:classification* / Q000592:standards; D007231:Infant, Newborn / Q000276:immunology*; D011247:Pregnancy; D013223:Statistics as Topic
D000911:Antibodies, Monoclonal; D007126:Immunoglobulin Allotypes; D007074:Immunoglobulin G