Abnormal monocyte chemotaxis in patients with chronic purulent rhinosinusitis: an effect of retroviral p15E-related factors in serum.
Earlier we reported that about 60% of patients suffering from unexplained relapsing of chronic purulent rhinosinusitis show a defective T cell-mediated immunity to commensal microorganisms of the upper respiratory tract. The monocyte chemotactic responsiveness was assessed in 40 of these patients by means of the polarization assay. Impaired FMLP-induced monocyte polarization was found in 26 of the 40 patients tested. The defective chemotactic responsiveness could be explained by a p15E-related factor detectable in the serum of the patients: addition of serum fractions less than 25 kD to healthy donor monocytes resulted in an inhibition of polarization; a monoclonal antibody directed against p15E neutralized this inhibitory effect. In individual patients, a decreased monocyte polarization correlated well with the presence of this p15E-related factor in serum, as well as with defective T cell reactivity.
van de Plassche-Boers
E M
EM
Laboratory for Clinical Immunology, Pathological Institute, Free University, Amsterdam, The Netherlands.
Tas
M
M
de Haan-Meulman
M
M
Kleingeld
M
M
Drexhage
H A
HA
D000328:Adult; D000911:Antibodies, Monoclonal; D002634:Chemotaxis, Leukocyte; D002908:Chronic Disease; D005260:Female; D015683:Gene Products, gag; D006801:Humans; D008297:Male; D008875:Middle Aged; D008970:Molecular Weight; D009000:Monocytes / Q000502:physiology*; D012191:Retroviridae Proteins / Q000097:blood* / Q000276:immunology; D015682:Retroviridae Proteins, Oncogenic; D012852:Sinusitis / Q000097:blood* / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology
D000911:Antibodies, Monoclonal; D015683:Gene Products, gag; D012191:Retroviridae Proteins; D015682:Retroviridae Proteins, Oncogenic; C039467:virus structural protein p15