Structure and expression of oncogenes in surgical specimens of human breast carcinomas.
We have performed an analysis of ras, c-myc, c-myb, c-erbB1 and c-erbB2 oncogenes in 100 surgical samples of human breast carcinomas. No point mutations have been detected at the 12th codon of c-Ha-ras and c-Ki-ras in 40 and 65 breast cancer DNAs, respectively. One out of 65 samples showed a 50-fold amplification of c-Ha-ras that, however, was not overexpressed. Alterations in the structure of c-myc, c-myb c-erbB1 and c-erbB2 oncogenes were sporadically observed. In 20 tumour samples, the study of expression of a series of oncogenes revealed that c-Ha-ras was the predominantly transcribed gene among the ras gene family whereas c-fos appeared the most constantly and significantly expressed nuclear oncogene.
Biunno
I
I
Division of Experimental Oncology A, Istituto Nazionale Tumori, Milan, Italy.
Pozzi
M R
MR
Pierotti
M A
MA
Pilotti
S
S
Cattoretti
G
G
Della Porta
G
G
D000483:Alleles; D001943:Breast Neoplasms / Q000235:genetics*; D003062:Codon; D004587:Electrophoresis, Agar Gel; D005260:Female; D005784:Gene Amplification; D011905:Genes, ras; D006801:Humans; D009154:Mutation; D011519:Proto-Oncogenes
D003062:Codon