[Procedure for identifying atypical variants of pseudocholinesterase for the prevention of prolonged apnea induced by succinylcholine].
The simple Sclavo test based on succinyldicholine hydrolysis and on the following reaction with 5,5'-dithio-bis-2-nitrobenzoic acid for detection of the atypical patterns of serum cholinesterase has been investigated. A yellow staining appears but in presence of the atypical forms of the enzyme. Analysis of 4055 serum samples by the 430 Selective Analyzer Sclavo allowed the identification of one atypical homozygous patient. Prolonged apnoea induced by use of succinyldicholine as a neuromuscular blocking agent during anaesthesia may be avoided by use of this test.
Grisler
R
R
Laboratorio di ricerche cliniche, anatomia ed istologia patologica, Istituti clinici di perfezionamento, Milano.
Santeramo
C
C
Scaricabarozzi
R
R
D001049:Apnea / Q000139:chemically induced / Q000201:enzymology / Q000235:genetics / Q000517:prevention & control*; D002091:Butyrylcholinesterase / Q000097:blood* / Q000145:classification; D002802:Cholinesterases / Q000097:blood*; D006801:Humans; D013390:Succinylcholine / Q000009:adverse effects*
D002091:Butyrylcholinesterase; D002802:Cholinesterases; D013390:Succinylcholine
Procedimento per l'individuazione delle varianti atipiche della pseudocolinesterasi ai fini della prevenzione dell'apnea protratta indotta dalla succinildicolina.