Tolerance to minor histocompatibility antigens.
A neonatal tolerance model employing fully allogeneic lymphoid cells as tolerogen was used in an investigation of tolerance to self and donor minor histocompatibility antigens (miHA). Tolerance was assessed by skin grafting and subsequently by the generation of cytotoxic T lymphocytes. Two strain combinations were investigated. In the first, BALB/c-B10, none of the mice became tolerant to H-2d: all gave responses to BALB/c and B10.D2 antigens comparable to uninjected controls. However, tolerance was secured to BALB miHA in the face of reactivity to the original donor cells (i.e., BALB/c), showing that multiple miHA can induce tolerance independently of major histocompatibility complex (MHC) antigens. In the reverse strain combination, in which tolerance to B10 antigens was successfully established in BALB/c recipients, MHC restriction of tolerance to self miHA could not be demonstrated, as mice tolerant to B10 were unresponsive to BALB.B antigens, too. Again, the induction of tolerance to multiple donor miHA proved to be independent of tolerance to donor MHC antigens, and a great deal easier.
Rayfield
L S
LS
Department of Immunology, St. Mary's Hospital Medical School, London, U.K.
Brent
L
L
Samuel
K
K
D000818:Animals; D000831:Animals, Newborn; D016026:Bone Marrow Transplantation; D003602:Cytotoxicity, Immunologic; D006084:Graft Rejection; D006183:H-2 Antigens / Q000276:immunology; D006649:Histocompatibility Antigens / Q000276:immunology*; D007108:Immune Tolerance; D051379:Mice; D008815:Mice, Inbred Strains; D016038:Skin Transplantation; D013154:Spleen / Q000637:transplantation; D014184:Transplantation, Homologous
D006183:H-2 Antigens; D006649:Histocompatibility Antigens