[Comparative analysis of the structure of double-stranded RNA from killer strains of Saccharomyces cerevisiae].
Double-stranded RNAs (M and L molecules) of two strains of the killer system Saccharomyces cerevisiae M437 (wild type) and ski-5 (superkiller mutant) were studied by means of electron microscopy and high resolution thermal melting. The M molecules of the ski-5 mutant were by 100 b.p. shorter than those of M437. L molecules were of the same length for both strains. Analysis of the differential melting curves of L molecules showed that L molecules differ significantly in their nucleotide sequences, whereas M molecules were practically identical. It was found that M molecules contained a long AU region: that of M molecules of M 437 was 170-180 b.p. long and contained almost no GC pairs, whereas the AU region of M molecules of the ski-5 mutant was three times shorter and contained GC pairs.
Amosova
O A
OA
Chernyĭ
D I
DI
Liubchenko
Iu L
IuL
Duzhak
A B
AB
Lobova
N N
NN
D008854:Microscopy, Electron; D009154:Mutation; D012330:RNA, Double-Stranded / Q000032:analysis* / Q000648:ultrastructure; D012331:RNA, Fungal / Q000032:analysis* / Q000648:ultrastructure; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000648:ultrastructure
D012330:RNA, Double-Stranded; D012331:RNA, Fungal
Sravnitel'nyĭ analiz stroeniia dvuspiral'nykh RNK killernykh shtammov Saccharomyces cerevisiae.