Lysis induction of Escherichia coli by the cloned lysis protein of the phage MS2 depends on the presence of osmoregulatory membrane-derived oligosaccharides.
Expression of the cloned lysis protein of phage MS2, which is sufficient to lyse wild type Escherichia coli, does not cause lysis of mutants lacking the osmoregulatory membrane-derived oligosaccharides (MDO). The lysis gene product normally found in the membrane fraction was not stably inserted into the membranes of a mdoA mutant; rather degradation and release from the membrane occurred. Gentle plasmolysis of the MDO-lacking mutant clearly showed an increased periplasmic space as compared to wild type cells. It is concluded that the MDOs play an important role in maintaining a proper arrangement of inner and outer membrane, a prerequisite for a functional insertion of the MS2 lysis protein.
Höltje
J V
JV
Max-Planck-Institut für Entwicklungsbiologie, Abteilung Biochemie, Tübingen, Federal Republic of Germany.
Fiedler
W
W
Rotering
H
H
Walderich
B
B
van Duin
J
J
D001433:Bacteriolysis; D002462:Cell Membrane / Q000502:physiology; D003090:Coliphages / Q000235:genetics / Q000502:physiology*; D004926:Escherichia coli / Q000235:genetics / Q000502:physiology*; D009844:Oligosaccharides / Q000502:physiology*; D009994:Osmolar Concentration; D010957:Plasmids; D011994:Recombinant Proteins / Q000494:pharmacology*; D014764:Viral Proteins / Q000235:genetics / Q000502:physiology*
D009844:Oligosaccharides; D011994:Recombinant Proteins; D014764:Viral Proteins; C037227:lysis protein, Enterobacterio phage MS2