Inhibition of hydrogen peroxide release from activated macrophages by prior ingestion of erythrocytes or haemoglobin.
Production of hydrogen peroxide by mouse peritoneal macrophages activated with Corynebacterium parvum was induced by incubating the cells with opsonised zymosan. H2O2 release was reduced by 47% when macrophages were preincubated with opsonised sheep erythrocytes. A significant decrease also occurred when the cells were preincubated with heat-denatured haemoglobin, but not when preincubated with opsonised erythrocyte ghosts, even though the latter were taken up by the macrophages. The ability of macrophages in an infected lesion to destroy microorganisms may therefore be impaired by ingestion of extravasated erythrocytes.
Stewart
L S
LS
University Department of Bacteriology and Immunology, Western Infirmary, Glasgow, U.K.
Licéaga
J
J
Brock
J H
JH
D000818:Animals; D004912:Erythrocytes / Q000502:physiology*; D006454:Hemoglobins / Q000502:physiology*; D006861:Hydrogen Peroxide / Q000378:metabolism*; D008262:Macrophage Activation; D008264:Macrophages / Q000378:metabolism*; D012756:Sheep
D006454:Hemoglobins; D006861:Hydrogen Peroxide