Site-specific endonucleolytic cleavages and the regulation of stability of E. coli ompA mRNA.
The stability of ompA mRNA is growth-rate dependent. We show that the 5' noncoding region of this mRNA provides a target for site-specific endonucleases. The rate of degradation of ompA mRNA parallels the rate of these endonucleolytic cleavages, implying that endonucleolytic rather than exonucleolytic attack is the initial step in ompA mRNA degradation. Thus the 5' noncoding region appears to be a determinant of mRNA stability, and endonucleolytic cleavages in the 5' noncoding region may well regulate expression of the ompA gene.
Melefors
O
O
Institute for Applied Cell and Molecular Biology, Umeå University, Sweden.
von Gabain
A
A
D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D004722:Endoribonucleases / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics*; D005786:Gene Expression Regulation; D005796:Genes; D005798:Genes, Bacterial; D007700:Kinetics; D010957:Plasmids; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic
D001425:Bacterial Outer Membrane Proteins; D012333:RNA, Messenger; D004722:Endoribonucleases