Detection of dengue virus type 2 in Aedes albopictus by nucleic acid hybridization with strand-specific RNA probes.
A molecular hybridization technique with radiolabeled, strand-specific RNA probes was developed to detect dengue virus type 2 RNA in pools of infected Aedes albopictus mosquitoes. One infected mosquito in a pool of 25 could be detected, corresponding to a dengue virus type 2 titer of 2.75 log10 50% tissue culture infectious doses.
Olson
K
K
Department of Microbiology, Colorado State University, Fort Collins 80523.
Blair
C
C
Padmanabhan
R
R
Beaty
B
B
D000330:Aedes / Q000382:microbiology*; D000818:Animals; D003716:Dengue Virus / Q000235:genetics / Q000302:isolation & purification*; D005260:Female; D005455:Fluorescent Antibody Technique; D007303:Insect Vectors / Q000382:microbiology*; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000032:analysis*
D012367:RNA, Viral