title: Differential effects of tetracaine on charge movements and Ca2+ signals in frog skeletal muscle.
abstract: The effects of tetracaine on charge movements and on antipyrylazo III signals monitoring intracellular delta [Ca2+] were compared in cut frog semitendinosus muscle fibers in a single vaseline gap-voltage clamp. Low tetracaine concentrations (25-40 microM) markedly reduced delta [Ca2+] signals and shifted the rheobase. However, they neither influenced charge movement nor that peak delta [Ca2+] value associated with the contractile threshold. Higher tetracaine concentrations (100-200 microM) partly inhibited charge movements in cut fibers. They separated a steeply voltage-sensitive charge, some of whose features resembled 'q gamma' reported in intact fibers, and whose movement preceded delta [Ca2+] signals at threshold. These findings: (a) directly confirm an earlier suggestion that tetracaine acts on steps in excitation-contraction coupling rather than myofilament activation; (b) show that tetracaine at low concentrations can directly interfere with sarcoplasmic reticular calcium release without modifying charge movement; (c) show that the tetracaine-sensitive charge, first found in intact fibers, also exists in cut fibers; and (d) make it unlikely that tetracaine-sensitive charge transfer is a consequence of Ca2+ release as suggested on earlier occasions.
authors.affiliation: Department of Physiology, University Medical School, Debrecen, Hungary.
authors.forename: L
authors.initials: L
authors.lastname: Csernoch
authors.forename: C L
authors.initials: CL
authors.lastname: Huang
authors.forename: G
authors.initials: G
authors.lastname: Szucs
authors.forename: L
authors.initials: L
authors.lastname: Kovacs
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004594:Electrophysiology; D008564:Membrane Potentials / Q000187:drug effects; D009119:Muscle Contraction / Q000187:drug effects*; D009132:Muscles / Q000502:physiology; D011893:Rana esculenta; D013748:Tetracaine / Q000494:pharmacology*
chemical_list: D013748:Tetracaine; D002118:Calcium