Oncogene expression in related cancer lines differing in metastatic capacity.
Various murine tumor cell lines with different metastatic capacities were tested in vitro for oncogene expression, especially of the p21-Ha-ras protein. Small differences were seen in the expression of several distinct oncogenes in the case of a high metastatic lymphoma variant (ESb) and its low metastatic parental line (Eb). In one instance we observed a 30-fold Ha-ras gene amplification in a metastasis-derived cell line from a spontaneous mouse mammary carcinoma. In spite of this amplification we did not find an increased p21 expression in these cells.
Pohl
J
J
Institute for Immunology and Genetics, German Cancer Research Center, Heidelberg.
Radler-Pohl
A
A
Heicappell
R
R
Schirrmacher
V
V
D000818:Animals; D002460:Cell Line; D004273:DNA, Neoplasm / Q000032:analysis; D005784:Gene Amplification; D008325:Mammary Neoplasms, Experimental / Q000235:genetics* / Q000473:pathology; D051379:Mice; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D009362:Neoplasm Metastasis; D009857:Oncogenes; D011518:Proto-Oncogene Proteins / Q000032:analysis; D016283:Proto-Oncogene Proteins p21(ras); D012333:RNA, Messenger / Q000032:analysis
D004273:DNA, Neoplasm; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D016283:Proto-Oncogene Proteins p21(ras)