title: Removal, generation and adsorption of beta-2-microglobulin during hemofiltration with five different membranes.
abstract: Removal of beta 2-microglobulin (B2MG) from uremic plasma during hemofiltration in 5 patients using polysulfone (F60), acrylonitrile (AN69), polyamid (FH77), polyacrylonitrile (PAN200) and cellulose-acetate (Duo-Flux) membranes was investigated. The reduction in plasma concentration of B2MG (corrected for hemoconcentration during treatment) was greatest for the F60 hemofilter (61%, sieving coefficient 0.55 at end of treatment) and the AN69 (48%, sieving coefficient 0.45 at end of treatment, while the PAN200 membrane showed no reduction in plasma concentration of B2MG during treatment. Mass balance for B2MG showed that there was a continued net adsorption of B2MG in the AN69 membrane (about 120 mg) throughout the treatment, while insignificant amount of B2MG was absorbed by the F60 membrane. The results with the PAN200 showed a 'negative adsorption', indicating release of B2MG from cells into the plasma as blood passed through this filter. Comparison of reduction in plasma concentrations, sieving coefficients, plasma clearances and filter mass balances showed that the question of B2MG removal during hemofiltration is a complex issue where adsorption to the membrane, 'shedding' from blood cells, membrane pore size and possibly also 'redistribution' of B2MG in the body must be considered.
authors.affiliation: Department of Medicine, University of Trondheim, Norway.
authors.forename: S
authors.initials: S
authors.lastname: Jørstad
authors.forename: L C
authors.initials: LC
authors.lastname: Smeby
authors.forename: T
authors.initials: T
authors.lastname: Balstad
authors.forename: T E
authors.initials: TE
authors.lastname: Widerøe
mesh_terms: D000327:Adsorption; D006440:Hemofiltration / Q000592:standards; D006801:Humans; D008567:Membranes, Artificial; D013997:Time Factors; D014511:Uremia / Q000628:therapy; D001613:beta 2-Microglobulin / Q000032:analysis*
chemical_list: D008567:Membranes, Artificial; D001613:beta 2-Microglobulin