title: Comparison between enzyme-linked immunosorbent and I.F. assays in the serological diagnosis of HIV infections.
abstract: A rapid, sensitive indirect immunofluorescence assay based on the use of acetone-fixed virus producing MOLT T4/LAV cells was adapted for the detection of anti-HIV antibodies. At the same time, 1486 serum samples, collected by the AIDS Surveillance and Study Centre of Modena, were tested by ELISA and IFA: the percentage of agreement of both assays was of 99.66%. Such datum suggested that IFA could be used as a serological assay for screening and confirmatory purposes.
authors.affiliation: Department of Infectious Diseases, University of Modena, Italy.
authors.forename: V
authors.initials: V
authors.lastname: Borghi
authors.forename: M
authors.initials: M
authors.lastname: De Palma
authors.forename: M
authors.initials: M
authors.lastname: Vecchi
authors.forename: F
authors.initials: F
authors.lastname: Pellegrino
authors.forename: N
authors.initials: N
authors.lastname: Mongiardo
authors.forename: I
authors.initials: I
authors.lastname: Cocchi
authors.forename: B
authors.initials: B
authors.lastname: De Rienzo
authors.forename: F
authors.initials: F
authors.lastname: Squadrini
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000175:diagnosis*; D000914:Antibodies, Viral / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D005188:False Negative Reactions; D005189:False Positive Reactions; D005455:Fluorescent Antibody Technique; D006678:HIV / Q000276:immunology*; D015483:HIV Antibodies; D006801:Humans
chemical_list: D000914:Antibodies, Viral; D015483:HIV Antibodies