Requirement of Ia-positive nylon wool adherent cells for activation of cytotoxic T-lymphocytes specific to melanocyte-associated antigens in patients with Vogt-Koyanage-Harada's disease.
Involvement of Ia-positive nylon wool adherent cells (AC) for the expression of cytotoxic T-lymphocyte (CTL) activity specific to melanocyte-associated antigens (MAA) was studied. Although unseparated peripheral blood lymphocytes (PBL) from several patients with Vogt-Koyanagi-Harada's (VKH) disease showed significant cytotoxic activity against SK-MEL-28 (P-36) human melanoma cells which carried cross-reactive surface antigens with normal melanocytes, the cytotoxic activity of nylon wool column passed nonadherent cells (NAC) against the same target cells markedly decreased in the absence of Ia-positive nylon wool AC. The reduced cytotoxic activity of the NAC was reconstituted not only by the addition of Ia-positive AC from the same VKH patient, but also by the addition of allogeneic IA-positive AC mismatched with human leukocyte antigen (HLA) complex-DR region haplotype. Moreover, the cytotoxic activity of nylon wool NAC was augmented by the addition of interleukins instead of DR-positive AC. The data indicate that the primed CTL in patients with VKH would be stimulated nonspecifically by factors from Ia-positive cells, presumably to differentiate the precursor CTL (CTL in memory state) into mature CTL (effector cells).
Maezawa
N
N
Ophthalmic Clinic, Showa-Inan General Hospital, Nagano, Japan.
Yano
A
A
D000328:Adult; D000911:Antibodies, Monoclonal; D000938:Antigen-Presenting Cells / Q000276:immunology; D002448:Cell Adhesion; D002450:Cell Communication; D002454:Cell Differentiation; D002458:Cell Fractionation; D002478:Cells, Cultured; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology; D008544:Melanocytes / Q000276:immunology; D008875:Middle Aged; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014605:Uveitis / Q000276:immunology*; D014607:Uveomeningoencephalitic Syndrome / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000949:Histocompatibility Antigens Class II; D007375:Interleukin-1