Effect of treatment medium on induction of aneuploidy by nocodazole in Saccharomyces cerevisiae.
While studying ways to improve responsiveness of Saccharomyces cerevisiae strain D61.M to agents that induce aneuploidy, we noted that nocodazole, which strongly induces aneuploidy when yeast cells are treated in yeast extract-peptone-dextrose (YEPD) medium, had no effect when a synthetic complete (SC) medium was used. Further study revealed that the presence of peptone was necessary for induction. Other aneuploidy-inducing agents, including ethyl acetate, acetone, and methyl benzimidazole-2-yl-carbamate (MBC), were equally active in either medium. Benomyl, which degrades to MBC, was less active in SC than in YEPD medium.
Taylor-Mayer
R E
RE
Biology Department, Slippery Rock University, Pennsylvania.
Mayer
V W
VW
Goin
C J
CJ
D000782:Aneuploidy / Q000187:drug effects*; D001562:Benzimidazoles / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D003470:Culture Media / Q000494:pharmacology*; D009153:Mutagens / Q000494:pharmacology; D015739:Nocodazole; D010461:Peptones / Q000494:pharmacology*; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics
D001562:Benzimidazoles; D003470:Culture Media; D009153:Mutagens; D010461:Peptones; D015739:Nocodazole