Investigations on the binding of erythrogenic toxin A of Streptococcus pyogenes on human peripheral blood lymphocytes. II. Identification of toxin-binding lymphocytes and characterization of the receptor.
Gold complexes of erythrogenic toxin A (ETA) of Streptococcus pyogenes (strain NY-5) were bound to human lymphocytes that could be characterized as T cells by rosetting or labelling with OKT 3 antibody and anti-mouse-IgG-gold. Using OKT 4 and OKT 8 antibodies, respectively, it was demonstrated that binding of ETA was confined to the T helper/inducer cell subpopulation. Treatment of lymphocytes with neuraminidase enhanced the binding of ETA-gold, whereas trypsin treatment resulted in its reduction. Gangliosides of type II or type III and D-galactose only slightly decreased the ETA-gold binding. Preincubation of living lymphocytes with the mitogens concanavalin A or the Lens culinaris-lectin enhanced the binding of ETA-gold.
Wagner
B
B
Zentralinstitut für Mikrobiologie und experimentelle Therapie, Jena, DDR.
Scriba
S
S
Wagner
M
M
Köhler
W
W
D001426:Bacterial Proteins; D002478:Cells, Cultured; D003102:Colloids; D005098:Exotoxins / Q000378:metabolism*; D006046:Gold; D006801:Humans; D008565:Membrane Proteins; D008854:Microscopy, Electron; D011971:Receptors, Immunologic / Q000378:metabolism*; D012397:Rosette Formation; D013297:Streptococcus pyogenes; D013601:T-Lymphocytes / Q000378:metabolism* / Q000648:ultrastructure
D001426:Bacterial Proteins; D003102:Colloids; D005098:Exotoxins; D008565:Membrane Proteins; D011971:Receptors, Immunologic; C117980:SpeA protein, Streptococcus pyogenes; C027951:erythrogenic toxin; C049791:erythrogenic toxin A receptor; D006046:Gold