The use of the rat hepatoma cell line MH1C1 to investigate the stimulus-secretion response of hepatic acute phase proteins.
The rat hepatoma cell line MH1C1 has been characterized to show a stimulated secretion of C-reactive protein in response to both leukocyte supernatant and a purified human interleukin-1 preparation. The time-dependency and dose-response relationship of CRP secretion were comparable to and somewhat more sensitive than the effects of leukocyte supernatant and purified human interleukin-1 on the proliferative rate of murine thymocytes; the proliferative rate of the hepatoma cell line MH1C1 was unchanged under these conditions. Agents which affect the thymocyte bioassay response to interleukin-1 namely interleukin-2, lipopolysaccharide, concanavalin A and phytohemagglutinin showed no effect on the C-reactive protein release of the MH1C1 cell line. These data strongly support the suitability of this cell line for the in vitro study of the hepatic acute phase stimulus-secretion response.
Taktak
Y S
YS
Department of Chemical Pathology, Old Medical School, Leeds University, United Kingdom.
Grant
A M
AM
D000818:Animals; D001681:Biological Assay; D002097:C-Reactive Protein / Q000378:metabolism*; D002455:Cell Division; D003208:Concanavalin A / Q000494:pharmacology; D003470:Culture Media; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007375:Interleukin-1 / Q000032:analysis; D007376:Interleukin-2 / Q000494:pharmacology; D007962:Leukocytes; D008070:Lipopolysaccharides / Q000494:pharmacology; D008114:Liver Neoplasms, Experimental / Q000378:metabolism*; D051379:Mice; D008809:Mice, Inbred C3H; D010835:Phytohemagglutinins / Q000494:pharmacology; D051381:Rats; D013950:Thymus Gland / Q000166:cytology; D014407:Tumor Cells, Cultured / Q000378:metabolism
D003470:Culture Media; D007375:Interleukin-1; D007376:Interleukin-2; D008070:Lipopolysaccharides; D010835:Phytohemagglutinins; D003208:Concanavalin A; D002097:C-Reactive Protein