Improved quantitation of delayed-type hypersensitivity responses to sheep erythrocytes in the mouse footpad by estimation of 125I-fibrin deposition.
Footpad swelling in response to subcutaneous antigen challenge is widely used to assess delayed-type hypersensitivity responses to xenogeneic erythrocytes in mice. We report here that quantitation of the deposition of 125I-fibrin, compared with that of 125I-human serum albumin, following intravenous isotope administration, provides a less subjective, convenient and antigen specific index of cell-mediated immunity. Moreover, the results obtained correlate well with in vitro assessment of antigen-specific T cell responses.
Webster
L M
LM
Department of Pathology, University of Aberdeen, Scotland, U.K.
Thomson
A W
AW
D000818:Animals; D004912:Erythrocytes; D005337:Fibrin; D006968:Hypersensitivity, Delayed / Q000276:immunology*; D007111:Immunity, Cellular; D007158:Immunologic Techniques; D007457:Iodine Radioisotopes; D051379:Mice; D012709:Serum Albumin; D012756:Sheep
D007457:Iodine Radioisotopes; D012709:Serum Albumin; D005337:Fibrin