An enzyme immunoassay for cuprozinc superoxide dismutase using monoclonal antibodies. Application for pharmacokinetic study.
We developed an enzyme immunoassay for determining human cuprozinc superoxide dismutase (h-SOD) using two kinds of monoclonal antibodies prepared by immunizing h-SOD to BALB/c mice. This method was sensitive and specific enough to determine exogenous h-SOD injected into rats. When intravenously injected into rats, much of the immunoreactive h-SOD accumulated in the kidney and was rapidly excreted in the urine. We observed both a modified and an unmodified form of exogenous h-SOD in rat urine.
Adachi
T
T
Department of Pharmaceutics, Gifu Pharmaceutical University, Japan.
Usami
Y
Y
Kishi
T
T
Hirano
K
K
Hayashi
K
K
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis; D002417:Cattle; D002850:Chromatography, Gel; D004285:Dogs; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D007668:Kidney / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains; D013482:Superoxide Dismutase / Q000276:immunology / Q000493:pharmacokinetics / Q000652:urine*; D013552:Swine; D013997:Time Factors
D000911:Antibodies, Monoclonal; D013482:Superoxide Dismutase