title: A comparison of culture, direct fluorescent antibody test, and a quantitative indirect immunoperoxidase assay for detection of Chlamydia trachomatis in pregnant women.
abstract: An indirect immunoperoxidase assay that quantitates antichlamydial immunoglobulin G (IgG) and IgA antibodies in serum was compared with endocervical culture and direct fluorescent antibody (MicroTrak) for detection of Chlamydia trachomatis in asymptomatic pregnant women. Of the 64 women tested by the three methods, 22 (34%) had antichlamydial IgG and IgA in their serum. The culture was positive in nine patients (14%) and the MicroTrak was positive in eight (12.5%). All positive cultures were immunoperoxidase-positive. One positive MicroTrak was immunoperoxidase- and culture-negative. Thirteen patients had IgG and IgA antichlamydial antibodies with no organisms detected in the endocervix by culture or by direct fluorescent antibody screen.
authors.affiliation: Department of Obstetrics and Gynecology, State University of New York Health Science Center, Syracuse.
authors.forename: N G
authors.initials: NG
authors.lastname: Osborne
authors.forename: Y
authors.initials: Y
authors.lastname: Hecht
authors.forename: J
authors.initials: J
authors.lastname: Gorsline
authors.forename: B A
authors.initials: BA
authors.lastname: Forbes
authors.forename: F
authors.initials: F
authors.lastname: Morgenstern
authors.forename: J
authors.initials: J
authors.lastname: Winkelman
mesh_terms: D002478:Cells, Cultured; D002690:Chlamydia Infections / Q000175:diagnosis*; D002692:Chlamydia trachomatis / Q000302:isolation & purification; D005069:Evaluation Studies as Topic; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007124:Immunoenzyme Techniques; D011247:Pregnancy; D011248:Pregnancy Complications; D011933:Reagent Kits, Diagnostic
chemical_list: D011933:Reagent Kits, Diagnostic