Proliferation of thymocytes in relation to T-cell receptor beta-chain expression.
During proliferation and differentiation of maturing thymocytes, T-cell receptor beta-chain products are first expressed in the cytoplasm. Only subsequently are they expressed on the cell surface, presumably as part of the alpha beta/CD3 receptor complex. This study uses double immunofluorescence labelling to identify these cytoplasmic and surface phases separately in relationship to cell-cycle parameters. The use of a mitotic arrest agent and tritiated thymidine autoradiography both show that cells with cytoplasmic beta-chains are in cell cycle, whereas cells with surface beta-chains are cycling slowly, if at all.
Parkin
I G
IG
Department of Anatomy, Medical School, University of Birmingham, U.K.
Owen
J J
JJ
Jenkinson
E J
EJ
D000818:Animals; D002453:Cell Cycle; D002462:Cell Membrane / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D051379:Mice; D008808:Mice, Inbred CBA; D008938:Mitosis / Q000187:drug effects; D011948:Receptors, Antigen, T-Cell / Q000096:biosynthesis*; D013601:T-Lymphocytes / Q000378:metabolism*; D013936:Thymidine / Q000378:metabolism; D014750:Vincristine / Q000494:pharmacology
D011948:Receptors, Antigen, T-Cell; D014750:Vincristine; D013936:Thymidine