New culture tube with an inside wall devised for studies of short-term hyperthermia.
We devised a new kind of culture tube with which a cell suspension could be warmed through both the inside and outside walls. Use of this tube makes it possible to measure the effects of short-term hyperthermia more accurately than with the conventional tubes, which is heated through the outside wall only. Temperature curves rose significantly faster in the double-walled tube than in the conventional one. The accelerated heating efficiency of the new tube was also seen by the enhanced inhibition of [3H]thymidine incorporation into the DNA of Ehrlich ascites tumour cells grown in these tubes at the same final temperature as in a conventional tube.
Kageyama
K
K
Radioisotope Centre, Osaka City University Medical School, Japan.
Onoyama
Y
Y
Nakanishi
M
M
Ito
K
K
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism; D002478:Cells, Cultured; D003584:Cytological Techniques / Q000295:instrumentation*; D004273:DNA, Neoplasm / Q000378:metabolism; D004867:Equipment Design; D005069:Evaluation Studies as Topic; D013814:Thermal Conductivity; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors
D004273:DNA, Neoplasm; D013936:Thymidine