Comparative analysis of gp41 antigens by enzyme-linked immunosorbent assays for detecting antibodies to human immunodeficiency virus type 1.
We have compared the antigenic qualities of human immunodeficiency virus type 1 (HIV-1) gp41 glycoprotein with a synthetic oligopeptide (peptide R21S) and a bacterially synthesized protein (protein 566), which are homologous with the N-terminal region of gp41, in enzyme-linked immunosorbent assays (ELISA) for detecting antibodies to HIV-1 in sera of patients with the acquired immunodeficiency syndrome (AIDS) or the aids-related complex (ARC). Although the use of all three types of antigens readily allowed the detection of antibodies in human sera, ELISA employing purified gp41 glycoprotein and the protein 566 were more specific and sensitive than the peptide R21S ELISA.
Zweig
M
M
Program Resources, Inc., National Cancer Institute-Frederick Cancer Research Facility, MD.
Bladen
S V
SV
Dubois
G C
GC
Samuel
K P
KP
Showalter
S D
SD
Papas
T S
TS
D000386:AIDS-Related Complex / Q000276:immunology; D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D015483:HIV Antibodies / Q000302:isolation & purification*; D015488:HIV Antigens / Q000032:analysis* / Q000138:chemical synthesis; D015700:HIV Envelope Protein gp41; D006679:HIV Seropositivity / Q000175:diagnosis / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology; D006801:Humans; D014759:Viral Envelope Proteins / Q000032:analysis* / Q000138:chemical synthesis
D015483:HIV Antibodies; D015488:HIV Antigens; D015700:HIV Envelope Protein gp41; D014759:Viral Envelope Proteins