title: The Duchenne muscular dystrophy gene product is localized in sarcolemma of human skeletal muscle.
abstract: Duchenne muscular dystrophy (DMD) and its milder form, Becker muscular dystrophy (BMD), are allelic X-linked muscle disorders in man. The gene responsible for the disease has been cloned from knowledge of its map location at band Xp21 on the short arm of the X chromosome. The product of the DMD gene, a protein of relative molecular mass 400,000 (Mr 400K) recently named dystrophin, has been reported to co-purify with triads of mouse and rabbit skeletal muscle when assayed using polyclonal antibodies raised against fusion proteins encoded by regions of mouse DMD complementary DNA. Here we show that antibodies directed against synthetic peptides and fusion proteins derived from the N-terminal region of human DMD cDNA strongly react with an antigen present in skeletal muscle sarcolemma on cryostat sections of normal human muscle biopsies. This immunoreactivity is reduced or absent in muscle fibres from DMD patients but appears normal in muscle fibres from patients with other myopathic diseases. The same antibodies specifically react with a 400K protein in sodium dodecyl sulphate (SDS) extracts of normal human muscle subjected to Western blot analysis. We conclude that the product of the DMD gene is associated with the sarcolemma rather than with the triads and speculate that it strengthens the sarcolemma by anchoring elements of the internal cytoskeleton to the surface membrane.
authors.affiliation: Genetics Department, Hospital for Sick Children, Toronto, Ontario, Canada.
authors.forename: E E
authors.initials: EE
authors.lastname: Zubrzycka-Gaarn
authors.forename: D E
authors.initials: DE
authors.lastname: Bulman
authors.forename: G
authors.initials: G
authors.lastname: Karpati
authors.forename: A H
authors.initials: AH
authors.lastname: Burghes
authors.forename: B
authors.initials: B
authors.lastname: Belfall
authors.forename: H J
authors.initials: HJ
authors.lastname: Klamut
authors.forename: J
authors.initials: J
authors.lastname: Talbot
authors.forename: R S
authors.initials: RS
authors.lastname: Hodges
authors.forename: P N
authors.initials: PN
authors.lastname: Ray
authors.forename: R G
authors.initials: RG
authors.lastname: Worton
mesh_terms: D000906:Antibodies / Q000276:immunology; D004247:DNA / Q000235:genetics; D016189:Dystrophin; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D009124:Muscle Proteins / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D009132:Muscles / Q000032:analysis*; D009136:Muscular Dystrophies / Q000235:genetics* / Q000378:metabolism; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000276:immunology; D011993:Recombinant Fusion Proteins / Q000276:immunology; D012508:Sarcolemma / Q000032:analysis*
chemical_list: D000906:Antibodies; D016189:Dystrophin; D008565:Membrane Proteins; D009124:Muscle Proteins; D010446:Peptide Fragments; D011993:Recombinant Fusion Proteins; D004247:DNA