[UV-A exposure of the human epidermis enhances the binding of antibodies to SSA/Ro in vitro].
Normal and psoriatic skin specimens (n = 15) were processed to give 4-micron-thick frozen sections and exposed to a single UV irradiation in vitro. We used monochromatic UV-B (313 nm) and UV-A (365 nm). The doses were as follows: 10(3)/10(4) J/m2 (UV-B) or 4.10(3)/8.10(4) J/m2 (UV-A). We investigated the effect on epidermal binding of antibodies to SSA/Ro using the indirect immunofluorescence technique. Untreated and UV-B-treated human skin failed to bind anti-SSA/Ro. UV-A exposure disclosed reticular or granular staining of epidermal nuclei and a perinuclear halo. The effect was nearly the same throughout the dosage range. The combination of UV-A and methoxsalen increased the intensity of staining. Normal and psoriatic skin behaved in the same way.
Wollina
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U
Klinik und Poliklinik für Hautkrankheiten, Bereich Medizin der Friedrich-Schiller-Universität Jena.
Hipler
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Schaarschmidt
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H
D001323:Autoantibodies / Q000032:analysis*; D001324:Autoantigens / Q000276:immunology*; D004817:Epidermis / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D006801:Humans; D066298:In Vitro Techniques; D011701:PUVA Therapy; D011565:Psoriasis / Q000188:drug therapy* / Q000276:immunology; D020733:RNA, Small Cytoplasmic; D012261:Ribonucleoproteins; D000099207:SS-A Antigen
D001323:Autoantibodies; D001324:Autoantigens; D020733:RNA, Small Cytoplasmic; D012261:Ribonucleoproteins; D000099207:SS-A Antigen; C116006:RO60 protein, human
UV-A-Exposition humaner Epidermis ermöglicht die Bindung von Antikörpern gegen SSA/Ro in vitro.