Putative termination sites for rDNA transcription in rice.
A clone bearing a 9.8 kb insert DNA containing the rDNA unit was identified by screening an EcoR1 library of rice DNA in lambda Charon 4 phage with [32P]-rRNAs. The S1 nuclease mapping of the rDNA-precursor rRNA hybrids showed the presence of two transcription termini on the rDNA. They were mapped at positions 616 and 620 nucleotides downstream to the end of the 25S rRNA gene. The 18 nucleotide sequence, where the transcription terminates on the rDNA in rice and mice are homologous albeit in the reverse orientation.
Nandabalan
K
K
Centre for Genetic Engineering, Indian Institute of Science, Bangalore.
Padayatty
J D
JD
D001483:Base Sequence; D003001:Cloning, Molecular; D004275:DNA, Ribosomal / Q000235:genetics*; D005809:Genes, Regulator; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics*; D012045:Regulatory Sequences, Nucleic Acid; D013728:Terminator Regions, Genetic; D014158:Transcription, Genetic
D004275:DNA, Ribosomal