Characterization of adenovirus particles made by deletion mutants lacking the fiber gene.
H2dl802, H2dl807, and H5dl1021 are defective deletion mutants of human adenovirus which do not make the capsid protein fiber yet which can make substantial amounts of virus particles. Virions made by the mutants contain very little fiber (which comes from helper virus contaminants in the deletion virus stocks): less than 6% as much as that contained by wild-type virions. This demonstrates that fiber is not an essential structural component of the adenovirus virion and suggests that fiber is nonessential for virion assembly. These fiber-deficient particles are poorly adsorbed to cells, consistent with the proposed role of fiber in virus attachment. Further, virion protein precursors, including that of the virion protease, are poorly processed in these particles, suggesting a relationship between the presence of fiber and the maturation of the virus particle.
Falgout
B
B
Department of Biology, Johns Hopkins University, Baltimore, Maryland 21218.
Ketner
G
G
D000260:Adenoviruses, Human / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D002213:Capsid / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics*; D002872:Chromosome Deletion; D003673:Defective Viruses / Q000235:genetics / Q000378:metabolism*; D003720:Densitometry; D004591:Electrophoresis, Polyacrylamide Gel; D005814:Genes, Viral; D006801:Humans; D009154:Mutation; D010447:Peptide Hydrolases / Q000378:metabolism; D011498:Protein Precursors / Q000378:metabolism; D014709:Vero Cells; D014771:Virion / Q000032:analysis* / Q000201:enzymology / Q000378:metabolism
D011498:Protein Precursors; D010447:Peptide Hydrolases