Lipoprotein lipase activity in skeletal muscle and adipose tissue of marathon runners after simple and complex carbohydrate-rich diets.
A comparison of the influence of simple and complex carbohydrate (CHO) consumption on adipose tissue- and skeletal muscle-lipoprotein lipase activity (AT-LPLA, SM-LPLA) was examined. Twenty male marathon runners were divided into two equal dietary groups: simple-CHO and complex-CHO. Half of the subjects in each group consumed either a low-CHO (15% energy [E] intake), or a mixed diet (50% CHO) for 3 days. Immediately following this dietary period, the subjects consumed a CHO-rich diet (70% E intake) predominant in simple-CHO or in complex-CHO for an additional 3 days. Thereafter, all subjects returned to a normal mixed diet. Skeletal muscle biopsies, adipose tissue aspirations, and venous blood samples were obtained prior to dietary manipulation (PRE), upon completion of the 6 day diet (POST I), and 2 weeks after returning to a normal diet (POST II). The samples were analysed for AT-LPLA, SM-LPLA, serum insulin, and free fatty acids (FFA), and blood glucose, and lactate. SM-LPLA fell 71% from PRE values of 0.39 +/- 0.30 mu mol.g-1.h-1 to POST I values of 0.11 +/- 0.09 mu mol.g-1.h-1 (means +/- SD) (p less than 0.05), after a complex-CHO diet. However, the simple-CHO diet did not alter SM-LPLA. AT-LPLA similarly decreased (p less than 0.05) after the complex-CHO diet, and no significant decrease was noted after the simple-CHO diet.(ABSTRACT TRUNCATED AT 250 WORDS)
Roberts
K M
KM
Faculty of Physical Education, University of Western Ontario, London, Canada.
Noble
E G
EG
Hayden
D B
DB
Taylor
A W
AW
D000273:Adipose Tissue / Q000201:enzymology*; D001786:Blood Glucose; D004040:Dietary Carbohydrates / Q000008:administration & dosage*; D005227:Fatty Acids / Q000097:blood; D006801:Humans; D007328:Insulin / Q000097:blood; D007773:Lactates / Q000097:blood; D019344:Lactic Acid; D008071:Lipoprotein Lipase / Q000378:metabolism*; D008297:Male; D009132:Muscles / Q000201:enzymology*; D012420:Running
D001786:Blood Glucose; D004040:Dietary Carbohydrates; D005227:Fatty Acids; D007328:Insulin; D007773:Lactates; D019344:Lactic Acid; D008071:Lipoprotein Lipase