[Analysis of the potentials of molecular hybridization of nucleic acids as a method of the laboratory diagnosis of influenza].
The possibilities of using the DNA copies of different genes of influenza A virus for the detection of virus-specific RNa by molecular dot hybridization have been studied. High specificity and sensitivity of the RNA determination techniques have been demonstrated, as well as the efficacy of using DNA probes with the sequences of conservative genes (polymerase, nucleoprotein and matrix genes) for the detection of influenza A virus subtypes H1N1, H2N2, H3N2 and probes with the copies of the corresponding hemagglutinin genes for the differential determination of subtypes H3N2 and H1N1. The complex analysis of nasopharyngeal washings has confirmed the efficacy of the dot hybridization method for epidemiological investigations, particularly for deciphering influenza outbreaks, especially those of mixed etiology.
Pliusnin
A z
Az
Rozhkova
S A
SA
Nolandt
O V
OV
Briantseva
E A
EA
Noskov
F S
FS
D000818:Animals; D002642:Chick Embryo; D004269:DNA, Bacterial / Q000235:genetics; D004274:DNA, Recombinant; D004279:DNA, Viral / Q000235:genetics*; D005814:Genes, Viral; D005821:Genetic Techniques; D006801:Humans; D009980:Influenza A virus / Q000235:genetics* / Q000302:isolation & purification; D007251:Influenza, Human / Q000175:diagnosis* / Q000382:microbiology; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D012367:RNA, Viral / Q000235:genetics*
D004269:DNA, Bacterial; D004274:DNA, Recombinant; D004279:DNA, Viral; D012367:RNA, Viral
Analiz vozmozhnosteĭ molekuliarnoĭ gibridizatsii nukleinovykh kislot kak metod laboratornoĭ diagnostiki grippa.