The effect of saline loading on uranium-induced acute renal failure in rats.
Studies were performed to examine the effect of saline loading on uranium-induced acute renal failure (ARF) in rats. Forty-eight hours after the i.v. injection of uranyl acetate (UA, 5 mg/kg), inulin clearance rate (Cin) decreased to approximately 43% of the control value in water drinking rats (P less than 0.005). Animals receiving continuous isotonic saline infusion following UA showed higher urine flow and Cin (60% of control, P less than 0.01), and lessened intratubular cast formation when compared with water-drinking ARF rats. A short-term saline infusion following UA did not attenuate the decline in Cin (43% of control). An inverse relationship was found between Cin and the number of casts (r = -0.75, P less than 0.01). Multiple regression analysis showed that standardized partial regression coefficient is statistically significant between Cin and cast formation (-0.69, P less than 0.05), but not between Cin and tubular necrosis (-0.07, P greater than 0.05). Renin depletion caused by DOCA plus saline drinking did not attenuate the decline in Cin in ARF (47% of control). No significant difference was found in urinary uranium excretion between water-drinking and saline-infused ARF rats. The findings suggest that continuous saline infusion following UA attenuates the decline in Cin in ARF rats; and that this beneficial effect of saline loading is associated with lessened cast formation rather than with suppressed renin-angiotensin activity or enhanced urinary-uranium excretion.
Hishida
A
A
First Department of Medicine, Hamamatsu University School of Medicine, Japan.
Yonemura
K
K
Ohishi
K
K
Yamada
M
M
Honda
N
N
D058186:Acute Kidney Injury / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D000818:Animals; D007444:Inulin / Q000493:pharmacokinetics; D008297:Male; D009942:Organometallic Compounds / Q000633:toxicity*; D051381:Rats; D011919:Rats, Inbred Strains; D012083:Renin / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology*; D014501:Uranium / Q000652:urine
D009942:Organometallic Compounds; C005460:uranyl acetate; D012965:Sodium Chloride; D014501:Uranium; D007444:Inulin; D012083:Renin