Cloning and characterization of a cDNA for murine macrophage inflammatory protein (MIP), a novel monokine with inflammatory and chemokinetic properties.
In the course of studies on cachectin/TNF being conducted in our laboratory, a novel macrophage product has been detected and characterized. Termed macrophage inflammatory protein or MIP, this protein appears to be an endogenous mediator of the inflammatory events induced by endotoxin. A cDNA cloned probe for this protein has been isolated from a lambda gt10 phage library prepared from poly(A)+ RNA obtained of endotoxin-induced RAW264.7 cells. The sequence codes for a 92 amino acid-long polypeptide, of which 69 amino acids correspond to the mature product. The sequence predicts a molecular weight of 7,889 and structural analysis of the protein indicates a characteristic signal sequence alpha-helix and a hydrophobic core. Sequence data also confirm no sequence similarity to any other protein listed in the Dayhoff data base.
Davatelis
G
G
Laboratory of Medical Biochemistry, Rockefeller University, New York, New York 10021.
Tekamp-Olson
P
P
Wolpe
S D
SD
Hermsen
K
K
Luedke
C
C
Gallegos
C
C
Coit
D
D
Merryweather
J
J
Cerami
A
A
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001688:Biological Products / Q000235:genetics*; D002460:Cell Line; D002630:Chemotactic Factors / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D016209:Interleukin-8; D008070:Lipopolysaccharides / Q000494:pharmacology; D008213:Lymphocyte Activation; D051379:Mice; D008969:Molecular Sequence Data; D015846:Monokines; D012333:RNA, Messenger / Q000235:genetics
D001688:Biological Products; D002630:Chemotactic Factors; D016209:Interleukin-8; D008070:Lipopolysaccharides; D015846:Monokines; D012333:RNA, Messenger; D004247:DNA