title: Effects of hematopoietin-1 and interleukin 1 activities on early hematopoietic cells of the bone marrow.
abstract: Hematopoietin-1 (H-1) was purified from the human cell line 5637 and two amino acid sequences were observed in the preparation. One sequence was identical to that of interleukin 1 alpha (IL 1 alpha) and the other to that of IL 1 beta. The action of recombinant IL 1 alpha and other hematopoietic growth factors was studied using (a) a high proliferative potential colony-forming cell assay that uses primitive hematopoietic precursors from bone marrow, and (b) a spleen colony-forming unit assay. The results indicate that the IL 1 alpha target cell population is different than the target cell populations of IL 3, granulocyte-macrophage colony-stimulating factor; that IL 1 alpha in combination with mononuclear phagocyte colony-stimulating factor provides a proliferative stimulus; and that IL 1 alpha has at least a survival-enhancing and possibly proliferation-inducing effect on primitive hematopoietic stem cells.
authors.affiliation: Amgen, Thousand Oaks, CA 91320.
authors.forename: K M
authors.initials: KM
authors.lastname: Zsebo
authors.forename: J
authors.initials: J
authors.lastname: Wypych
authors.forename: V N
authors.initials: VN
authors.lastname: Yuschenkoff
authors.forename: H
authors.initials: H
authors.lastname: Lu
authors.forename: P
authors.initials: P
authors.lastname: Hunt
authors.forename: P P
authors.initials: PP
authors.lastname: Dukes
authors.forename: K E
authors.initials: KE
authors.lastname: Langley
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001853:Bone Marrow / Q000378:metabolism; D001854:Bone Marrow Cells; D003114:Colony-Forming Units Assay; D003115:Colony-Stimulating Factors / Q000096:biosynthesis / Q000302:isolation & purification / Q000494:pharmacology*; D005260:Female; D006133:Growth Substances / Q000302:isolation & purification / Q000494:pharmacology*; D016298:Hematopoietic Cell Growth Factors; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D006801:Humans; D007375:Interleukin-1 / Q000302:isolation & purification / Q000494:pharmacology*; D051379:Mice; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000494:pharmacology; D013154:Spleen / Q000166:cytology
chemical_list: D003115:Colony-Stimulating Factors; D006133:Growth Substances; D016298:Hematopoietic Cell Growth Factors; D007375:Interleukin-1; D011994:Recombinant Proteins