An unusual phosphatidylinositol turnover pathway in noradrenaline-perfused rat hearts.
1. The phosphatidylinositol turnover pathway has been studied in noradrenaline-perfused rat hearts using anion-exchange high performance liquid chromatography. 2. The active calcium-releasing compound inositol-(1,4,5)trisphosphate was detected together with its degradation products inositol-(1,4)bisphosphate and inositol monophosphate. All these products increased in response to noradrenaline stimulation. 3. At noradrenaline perfusion times from 5 s to 20 min there was no appearance of inositol-(1,3,4,5)tetrakisphosphate or its degradation products: inositol-(1,3,4)trisphosphate or inositol-(1,3) and (3,4)bisphosphates. 4. These data suggest the absence of the inositol-(1,4,5)trisphosphate phosphorylation/dephosphorylation pathway in heart.
Woodcock
E A
EA
Monash University Department of Medicine, Prince Henry's Hospital, Melbourne, Victoria, Australia.
Smith
A I
AI
Wallace
C A
CA
White
L B
LB
D000804:Angiotensin II / Q000494:pharmacology; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D006321:Heart / Q000187:drug effects*; D066298:In Vitro Techniques; D007293:Inosine Triphosphate / Q000378:metabolism; D007295:Inositol Phosphates / Q000378:metabolism; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D009638:Norepinephrine / Q000494:pharmacology*; D010477:Perfusion; D010716:Phosphatidylinositols / Q000378:metabolism*; D010766:Phosphorylation; D051381:Rats; D011919:Rats, Inbred Strains
D007295:Inositol Phosphates; D010716:Phosphatidylinositols; C081655:inositol-1,3,4,5-tetrakisphosphate; D000804:Angiotensin II; D007293:Inosine Triphosphate; D009638:Norepinephrine