title: Electron microscopy study of Q beta replicase.
abstract: Purified preparations of Q beta replicase have been studied by electron microscopy using a negative staining technique, and a three-dimensional model of the enzyme molecule has been constructed. The molecule of this four-subunit protein appears to be a compact structure having a size of 100 +/- 10 A; it is subdivided into two unequal bipartite subparticles. The conclusion has been made that all the constituent subunits, including the ribosomal protein Sl, acquire a globular conformation when associated in the replicase complex.
authors.affiliation: Institute of Protein Research, Academy of Sciences of the USSR, Pushchino, Moscow Region.
authors.forename: N H
authors.initials: NH
authors.lastname: Berestowskaya
authors.forename: V D
authors.initials: VD
authors.lastname: Vasiliev
authors.forename: A A
authors.initials: AA
authors.lastname: Volkov
authors.forename: A B
authors.initials: AB
authors.lastname: Chetverin
mesh_terms: D003090:Coliphages / Q000201:enzymology*; D004926:Escherichia coli / Q000201:enzymology*; D008854:Microscopy, Electron; D011487:Protein Conformation; D011777:Q beta Replicase; D012316:RNA Nucleotidyltransferases
chemical_list: D011777:Q beta Replicase; D012316:RNA Nucleotidyltransferases