title: Genetic and phenotypic analyses indicating occurrence of the recN262 and radB101 mutations at the same locus in Escherichia coli.
abstract: The radB101 and recN262 mutations showed essentially identical phenotypes when compared in isogenic Escherichia coli strains for their effects on gamma and UV radiation survival and on conjugal recombination in a uvrA recB recC sbcB sbcC strain. Complementation tests involving attempts to reconstitute a radB+ recN+ strain by transductions between radB101 and recN262 donors and recipients, and tests involving plasmids carrying recN+ and recN::Tn1000 inserts, indicated that the radB and recN genes are identical. We suggest that the radB101 mutation now be referred to as recN2001.
authors.affiliation: Department of Radiation Oncology, Stanford University School of Medicine, California 94305.
authors.forename: N J
authors.initials: NJ
authors.lastname: Sargentini
authors.forename: K C
authors.initials: KC
authors.lastname: Smith
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D002874:Chromosome Mapping; D003227:Conjugation, Genetic; D004262:DNA Restriction Enzymes; D004926:Escherichia coli / Q000235:genetics* / Q000528:radiation effects; D005720:Gamma Rays; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D009154:Mutation; D010641:Phenotype; D011995:Recombination, Genetic; D014161:Transduction, Genetic; D014169:Transformation, Bacterial; D014466:Ultraviolet Rays
chemical_list: D001426:Bacterial Proteins; D004262:DNA Restriction Enzymes; C047879:RecN protein, Bacteria