title: Calmodulin-binding profiles for nebulin and dystrophin in human skeletal muscle.
abstract: Nebulin and dystrophin are two high-molecular-mass skeletal muscle proteins that have both been associated with the defective gene in Duchenne muscular dystrophy, although the function of neither protein is known. Other high-molecular-mass, calmodulin-binding proteins have recently been implicated in regulating calcium release from skeletal muscle. Western blots of human skeletal muscle biopsy samples were probed with biotinylated calmodulin; nebulin was identified as a prominent high-molecular-mass calmodulin-binding protein but dystrophin did not bind detectable amounts of biotinylated calmodulin. Dystrophin was absent in a Duchenne muscle biopsy.
authors.affiliation: Jerry Lewis Muscle Research Centre, Department of Paediatrics, Royal Postgraduate Medical School, London, England.
authors.forename: K
authors.initials: K
authors.lastname: Patel
authors.forename: P N
authors.initials: PN
authors.lastname: Strong
authors.forename: V
authors.initials: V
authors.lastname: Dubowitz
authors.forename: M J
authors.initials: MJ
authors.lastname: Dunn
mesh_terms: D002147:Calmodulin / Q000378:metabolism*; D016189:Dystrophin; D006801:Humans; D008970:Molecular Weight; D009124:Muscle Proteins / Q000378:metabolism*; D009132:Muscles / Q000378:metabolism*; D009136:Muscular Dystrophies / Q000378:metabolism; D011485:Protein Binding; D012016:Reference Values
chemical_list: D002147:Calmodulin; D016189:Dystrophin; D009124:Muscle Proteins; C035620:nebulin