A rapid preparation of plasmid DNA from Saccharomyces cerevisiae.
A procedure for extraction of plasmid DNA from Saccharomyces cerevisiae is described. The plasmid DNA of interest is extracted together with 2-micron circular DNA naturally occurring in many yeast strains. Spheroplasts are lyzed at alkaline pH which denatures linear but not covalently closed circular (CCC) DNA. The CCC DNA is recovered by ethanol precipitation and can be detected by gel electrophoresis or used for routine bacterial transformation.
Brozmanová
J
J
Department of Molecular Genetics, Slovak Academy of Sciences, Bratislava, Czechoslovakia.
Holinová
Z
Z
D004271:DNA, Fungal / Q000302:isolation & purification*; D004926:Escherichia coli / Q000235:genetics; D007202:Indicators and Reagents; D008722:Methods; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D004271:DNA, Fungal; D007202:Indicators and Reagents