Production of alpha 1-antichymotrypsin by the J111 cell line.
The J111 histiocytic cell line is capable of producing human alpha 1-antichymotrypsin (alpha 1 ACh) in culture. The protein is secreted rather than stored in the cell, has a similar size to plasma alpha 1 ACh (68 kDa) and can complex with human cathepsin G. Dexamethasone and culture supernatants from U937 monocyte-like cells and alveolar macrophages, but not bacterial lipopolysaccharide, stimulate alpha 1ACh secretion by J111 cells. The J111 cell line thus provides a useful tool for the study of factors controlling alpha 1ACh synthesis in vivo.
Berman
G
G
Lung Immunobiochemical Research Laboratory, General Hospital, Birmingham.
Burnett
D
D
Woo
S L
SL
Stockley
R A
RA
D001345:Autoradiography; D002460:Cell Line; D003593:Cytoplasm / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008715:Methionine / Q000378:metabolism; D000514:alpha 1-Antichymotrypsin / Q000096:biosynthesis*
D000514:alpha 1-Antichymotrypsin; D008715:Methionine