Ellagic acid toxicity and interaction with benzo[a]pyrene and benzo[a]pyrene 7,8-dihydrodiol in human bronchial epithelial cells.
Ellagic acid, a plant phenol present in various foods consumed by humans, has been reported to have both anti-mutagenic and anti-carcinogenic potential. To evaluate the potential anti-carcinogenic property of ellagic acid, we tested its effects on the toxicity of benzo[a]pyrene and benzo[a]pyrene, 7,8-dihydrodiol and binding of benzo[a]pyrene to DNA in cultured human bronchial epithelial cells. The toxicity of ellagic acid itself for human bronchial epithelial cells was also determined. Using a colony-forming efficiency assay, it was found that a nontoxic concentration of ellagic acid (5 micrograms/ml) enhanced the toxicity of benzo[a]pyrene 7,8-dihydrodiol in human bronchial epithelial cells. In contrast, ellagic acid at concentrations of 1.5 and 3.0 micrograms/ml inhibited binding of benzo[a]pyrene metabolites to DNA in these cells. An explanation for the potentiating effect of ellagic acid on the toxicity of benzo[a]pyrene, 7,8-dihydrodiol will require further investigation into the possible mechanisms of interaction between these two compounds.
Teel
R W
RW
Department of Physiology, School of Medicine, Loma Linda University, California 92350.
Babcock
M S
MS
Dixit
R
R
Stoner
G D
GD
D001564:Benzo(a)pyrene / Q000378:metabolism / Q000633:toxicity*; D001578:Benzopyrans / Q000633:toxicity*; D001980:Bronchi / Q000166:cytology* / Q000187:drug effects; D002273:Carcinogens / Q000633:toxicity*; D002478:Cells, Cultured; D004247:DNA / Q000378:metabolism*; D004101:Dihydroxydihydrobenzopyrenes / Q000378:metabolism / Q000633:toxicity*; D004610:Ellagic Acid / Q000633:toxicity*; D006801:Humans; D014410:Tumor Stem Cell Assay
D001578:Benzopyrans; D002273:Carcinogens; D004101:Dihydroxydihydrobenzopyrenes; C017228:benzo(a)pyrene 7,8-dihydrodiol; D004610:Ellagic Acid; D001564:Benzo(a)pyrene; D004247:DNA