Effect of insulin on sulfated proteoglycan synthesis in cultured smooth muscle cells from pig aorta.
The effect of insulin upon proteoglycan synthesis was studied in cultured smooth muscle cells from pig aorta blocked in the G0 phase by serum deprivation. Insulin enhanced [35S]sulfate incorporation into cell layer and medium-secreted proteoglycans. The increase in incorporation of the precursor was not due to a mitogenic response by smooth muscle cells to the hormone and the specific radioactivity of proteoglycans showed that the stimulation reflected a real increase in sulfated proteoglycan synthesis. Maximal stimulation was observed, for the cell layer as well as for the medium, 40 h after the addition of 1.7 x 10(-7) M insulin and reached respectively 65 and 53%. This stimulation was about 80 and 60% of the level achieved with 10% fetal calf serum for cell layer and medium-secreted proteoglycans, respectively. The half-maximal effect was attained, for both the cell layer and the medium, in the presence of 2.1 x 10(-9) M insulin. Proteoglycans secreted into the medium, in the presence of 1.7 x 10(-8) M insulin for 40 h, showed a higher proportion of complexes (24%) than those synthesized in control medium (11%) and at least 95% of the monomers from culture treated with insulin were characterized by a smaller hydrodynamic size than those synthesized by cells maintained in control medium. This decrease in the size of proteoglycans was partly due to a decrease in the size of their glycanic chains.
Breton
M
M
Laboratoire de Biochimie, INSERM-U. 181, Faculté de Médecine, Saint-Antoine, Paris, France.
Berrou
E
E
Deudon
E
E
Brahimi-Horn
M C
MC
Picard
J
J
D000818:Animals; D001013:Aorta, Thoracic / Q000378:metabolism; D002478:Cells, Cultured; D002845:Chromatography; D006025:Glycosaminoglycans / Q000096:biosynthesis; D007328:Insulin / Q000494:pharmacology*; D007399:Interphase; D008970:Molecular Weight; D009130:Muscle, Smooth / Q000187:drug effects / Q000378:metabolism*; D011509:Proteoglycans / Q000096:biosynthesis*; D013431:Sulfates / Q000378:metabolism; D013552:Swine
D006025:Glycosaminoglycans; D007328:Insulin; D011509:Proteoglycans; D013431:Sulfates; C013786:A73025