title: Gel retardation at low pH resolves trp repressor-DNA complexes for quantitative study.
abstract: The affinity and stoichiometry of DNA binding by Escherichia coli trp repressor were studied by electrophoresis in nondenaturing gels. The ability of trp repressor to retard the electrophoretic mobility of an operator DNA fragment depends on the pH of the gel system. Above the pI of the protein, little retardation of DNA is observed, although complex formation can be detected by other assays. As the pH of the gel is lowered, retardation is enhanced. The apparent dissociation constant for the interaction between trp repressor and trpEDCBA operator fragments is 0.5 nM under the conditions used here. Nonspecific binding occurs with only about 200-fold weaker affinity. The stoichiometries of specific and nonspecific complexes were determined directly by using trp repressor labeled in vivo. High-affinity operator binding requires a single dimer of trp repressor. DNase I-protection analysis ("footprinting") was used to confirm the dissociation constants and to locate the binding site.
authors.affiliation: Department of Biochemistry, Stanford University Medical Center, CA 94305-5307.
authors.forename: J
authors.initials: J
authors.lastname: Carey
mesh_terms: D001426:Bacterial Proteins / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D004926:Escherichia coli / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D009875:Operator Regions, Genetic; D011485:Protein Binding; D011489:Protein Denaturation; D012097:Repressor Proteins / Q000302:isolation & purification*; D014157:Transcription Factors / Q000302:isolation & purification*
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D012097:Repressor Proteins; D014157:Transcription Factors