title: Blood group ABO-related antigens in fetal and normal adult bladder urothelium. Immunohistochemical study of type 2 chain structures with a panel of mouse monoclonal antibodies.
abstract: Seven monoclonal antibodies with specificity for blood group antigens carried by type 2 chain core structures (N-acetyllactosamine, H, Le(x), LeY, A monofucosylated, A difucosylated (AleY), A type 3) were used to study the distribution of these antigens in normal human bladder urothelium. The urothelial samples were from 7 fetuses (aged 7 to 21 weeks), and 22 adults. By means of an immunohistochemical method applied to frozen and fixed tissues, localization of antigens in the 3 cell layers of urothelium was compared with individual ABO, Lewis, and Secretor type. During fetal development, N-acetyllactosamine, H, and LeY antigens were continuously expressed, whereas Le(x) varied with age. The A type 3 chain was present in fetal urothelium but A type 2 chain was not. In adults, we observed a unique blood group distribution: with complex antigens like LeY and ALeY in all cell layers, and a unique expression of most of the less complex antigens in the luminal cell layer. Pretreatment of tissue sections with neuraminidase proved that Le(x) and N-acetyllactosamine could be partly substituted by sialic acid A type 3 chain and most of N-acetyllactosamine antigens were present only in cytoplasm, whereas all other examined antigens could be present both in cytoplasm and on cell membranes. Blood group A antigen expression was related to erythrocyte A1 and A2 subtype, and a remarkable correlation was found between type 1 chain related secretor status and expression of Le(x), LeY, and A antigens.
authors.affiliation: Department of Experimental Clinical Oncology, Danish Cancer Society, Aarhus, Denmark.
authors.forename: T F
authors.initials: TF
authors.lastname: Orntoft
authors.forename: H
authors.initials: H
authors.lastname: Wolf
authors.forename: H
authors.initials: H
authors.lastname: Clausen
authors.forename: S
authors.initials: S
authors.lastname: Hakomori
authors.forename: E
authors.initials: E
authors.lastname: Dabelsteen
mesh_terms: D000017:ABO Blood-Group System / Q000276:immunology*; D000328:Adult; D000911:Antibodies, Monoclonal; D002454:Cell Differentiation; D004847:Epithelial Cells; D004848:Epithelium / Q000276:immunology; D005333:Fetus / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques; D007519:Isoantigens / Q000032:analysis* / Q000378:metabolism; D007983:Lewis Blood Group Antigens / Q000276:immunology; D001743:Urinary Bladder / Q000166:cytology / Q000196:embryology / Q000276:immunology*
chemical_list: D000017:ABO Blood-Group System; D000911:Antibodies, Monoclonal; D007519:Isoantigens; D007983:Lewis Blood Group Antigens