title: Cooperative thermal denaturation of the assembly origin region of TMV RNA.
abstract: The assembly origin (AO) region of the tobacco mosaic virus RNA melts in an usually narrow (2.5 degrees C) temperature range. In an 0.01 M phosphate buffer the melting temperature of AO was found to be 41.5 degrees C. This value corresponds to the regions with the most stable secondary/tertiary structure of the whole TMV RNA molecule. It is assumed that the AO region has a specific tertiary structure, which is maintained by the long-range interactions as well as by interactions of the pseudoknot type.
authors.affiliation: Institute of Experimental Biology Academy of Sciences, Estonia, USSR.
authors.forename: M
authors.initials: M
authors.lastname: Kelve
authors.forename: A
authors.initials: A
authors.lastname: Aruja
authors.forename: K
authors.initials: K
authors.lastname: Kooli
authors.forename: J
authors.initials: J
authors.lastname: Männik
authors.forename: E
authors.initials: E
authors.lastname: Raukas
mesh_terms: D001483:Base Sequence; D005814:Genes, Viral; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation / Q000187:drug effects; D009691:Nucleic Acid Denaturation / Q000187:drug effects; D009994:Osmolar Concentration; D012367:RNA, Viral / Q000187:drug effects / Q000648:ultrastructure*; D012045:Regulatory Sequences, Nucleic Acid; D012965:Sodium Chloride / Q000494:pharmacology; D013696:Temperature; D014027:Tobacco Mosaic Virus / Q000235:genetics
chemical_list: D012367:RNA, Viral; D012965:Sodium Chloride