An improved technique for the study of immunofluorescence using non-decalcified frozen guinea pig cochlea.
A technique for producing frozen sections from the non-decalcified guinea pig cochlea is described. The sections can be utilised for various immunofluorescent studies, including the detection of humoral antibodies against different cellular elements in patients with cochleo-vestibular disorders. The technique assures the preservation of the cochlear architecture and the tissue antigenicity, and it avoids damage to the fine cochlear structural details.
Soliman
A M
AM
Department of Otorhinolaryngology, University of Tübingen, Germany.
D000818:Animals; D000906:Antibodies / Q000032:analysis; D003051:Cochlea / Q000033:anatomy & histology / Q000276:immunology; D005455:Fluorescent Antibody Technique; D005629:Frozen Sections; D006168:Guinea Pigs; D008856:Microscopy, Fluorescence; D008867:Microtomy
D000906:Antibodies