Analysis of macromolecular ethylene oxide adducts.
Ethylene oxide is a carcinogenic compound which is also an ethylene metabolite. Ethylene oxide forms macromolecular adducts with proteins and nucleic acids. Targets in proteins are the amino acids cysteine, histidine and valine (if N-terminal, as in hemoglobin). The major DNA adduct is 7-(2-hydroxyethyl)-guanine. Methods for detection of this adduct include radiolabelling and GC-MS. The sensitivity of current GC/MS methods can be improved by selective enrichment of adducts from DNA samples. Studies in this direction are presently being performed.
Bolt
H M
HM
Institut für Arbeitsphysiologie, Universität Dortmund, Federal Republic of Germany.
Peter
H
H
Föst
U
U
D000478:Alkylation; D000818:Animals; D002273:Carcinogens / Q000032:analysis / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D005027:Ethylene Oxide / Q000032:analysis* / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D011506:Proteins / Q000378:metabolism
D002273:Carcinogens; D011506:Proteins; D004247:DNA; D005027:Ethylene Oxide