Quantitative correlation of parasitological and serological techniques for the diagnosis of Trypanosoma congolense infection in cattle.
A comparison was made between serological and parasitological techniques for the diagnosis of bovine trypanosomiasis in Zambia. Overall sero-prevalence rates as determined by IFAT and ELISA were respectively 2.7-fold and 2.9-fold greater then the percentage of samples found positive with the dark ground/phase contrast buffy coat technique (DG). The results obtained by the two serological techniques were found to be closely correlated (94.2%) agreement) and titres obtained by ELISA tended to be slightly higher than those obtained by IFAT. Linear regression analysis of the results obtained by the IFAT and DG techniques revealed a highly significant correlation. This finding would permit the use of only one of the techniques in an epidemiological survey and to extrapolate the results from the regression line.
Jongejan
F
F
UNDP/FAO, Animal Disease Control Project, Lusaka, Zambia.
Ooijen
C J
CJ
Zivkovic
D
D
ter Huurne
A A
AA
D000818:Animals; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D012698:Serologic Tests / Q000662:veterinary; D014348:Trypanosoma congolense; D014353:Trypanosomiasis, African / Q000175:diagnosis / Q000662:veterinary; D014354:Trypanosomiasis, Bovine / Q000175:diagnosis*; D015024:Zambia