title: Lack of correlation between the induction of donor class I and class II major histocompatibility complex antigens and graft rejection.
abstract: The induction of donor major histocompatibility complex (MHC) antigens on nonrejected and rejected rat renal allografts was compared at various times after transplantation in two strain combinations, DA-to-PVG and LEW-to-DA. Graft rejection was prevented by preoperative donor-specific blood transfusion (DST). Quantitative absorption analysis and immunohistology were performed using monoclonal antibodies specific for donor class I and class II MHC antigens. A significant increase in the expression of donor MHC antigens, both class I and class II, was demonstrated on nonrejected as well as rejected kidneys after transplantation. A kinetic analysis showed that induction of donor class I antigens was accelerated on the nonrejected grafts, and by day 5 the nonrejected kidneys showed increased expression of class I antigen when compared with the rejected grafts (a 37- vs. a 25-fold increase in expression). Increased expression of donor class I antigens persisted on the nonrejected grafts and was still detectable on long-term-surviving kidneys, 50 days after transplantation. The magnitude of class II antigen induction was similar on both rejected and nonrejected grafts (8-fold by 5 days after transplantation). Immunohistology demonstrated that class I and class II antigens were induced on identical structures in the kidney in both situations. In particular the vessel endothelia, which do not express class II antigens in normal kidney, become strongly positive in both rejected and nonrejected grafts 5 days after transplantation. Although renal allograft rejection is completely suppressed in rats given a single donor-specific blood transfusion before transplantation, graft survival cannot be explained by the lack of induction of donor MHC antigens. Donor MHC antigens are induced on these nonrejected kidney grafts, and therefore they could act as target molecules for the effector cells that mediate graft destruction. Thus the induction of donor MHC antigens on tissue allografts should not be considered as indicative of a rejection response resulting in graft destruction.
authors.affiliation: Nuffield Department of Surgery, University of Oxford, U.K.
authors.forename: K J
authors.initials: KJ
authors.lastname: Wood
authors.forename: A
authors.initials: A
authors.lastname: Hopley
authors.forename: M J
authors.initials: MJ
authors.lastname: Dallman
authors.forename: P J
authors.initials: PJ
authors.lastname: Morris
mesh_terms: D000818:Animals; D001803:Blood Transfusion; D002465:Cell Movement; D006084:Graft Rejection; D006085:Graft Survival; D006649:Histocompatibility Antigens / Q000032:analysis* / Q000096:biosynthesis / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000032:analysis* / Q000096:biosynthesis / Q000276:immunology; D007150:Immunohistochemistry; D007163:Immunosorbent Techniques; D007668:Kidney / Q000032:analysis / Q000276:immunology; D016030:Kidney Transplantation; D007700:Kinetics; D007962:Leukocytes / Q000502:physiology; D008297:Male; D051381:Rats; D011917:Rats, Inbred Lew; D014019:Tissue Donors; D014184:Transplantation, Homologous
chemical_list: D006649:Histocompatibility Antigens; D000949:Histocompatibility Antigens Class II; C038353:histocompatibility antigens RT, rat