Enzyme immunoassay for the macrocyclic trichothecene roridin A: production, properties, and use of rabbit antibodies.
Antisera against roridin A were prepared by using a roridin A-hemisuccinate derivative coupled to human serum albumin as the immunogen. Antibodies could be detected in the sera of the immunized rabbits as early as 4 weeks after the initial exposure. After one booster injection at week 14, high antibody titers were measured over a period of 21 weeks. The specificity and sensitivity of the antibodies were tested by using roridin A-hemisuccinate coupled to horseradish peroxidase as an enzyme-linked toxin in a competitive assay with a double-antibody solid phase. The assay was most specific for the tested macrocyclic trichothecenes, and the relative cross-reactivities with roridin A, roridin J, verrucarin A, satratoxin H, and satratoxin G were 1, 0.41, 0.15, 0.15, and 0.07, respectively. When 16 nonmacrocyclic trichothecenes were tested, only diacetylverrucarol (0.0015) and verrucarol (0.0005) showed minor cross-reactivity. The sensitivity of the enzyme immunoassay for the detection of roridin A was in the range of 5 to 50 ng/ml (0.16 to 1.6 ng per assay).
Märtlbauer
E
E
Institute for Hygiene and Technology of Food of Animal Origin, University of Munich, Federal Republic of Germany.
Gareis
M
M
Terplan
G
G
D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D000906:Antibodies / Q000032:analysis; D007106:Immune Sera / Q000276:immunology*; D007124:Immunoenzyme Techniques; D011817:Rabbits; D014255:Trichothecenes / Q000032:analysis / Q000276:immunology
D000900:Anti-Bacterial Agents; D000906:Antibodies; D007106:Immune Sera; D014255:Trichothecenes; C038849:roridin A