The effect of stem cell proliferation regulators demonstrated with an in vitro assay.
Spleen colony formation after transplantation of bone marrow cells into irradiated mice has been used as an assay for hematopoietic stem cells (CFU-S), but has serious limitations intrinsic to an in vivo assay. In this report we describe experiments using an in vitro clonogenic assay that is especially suitable for studies of stem cell regulation as defined growth factors and normal untreated bone marrow can be used. We have demonstrated that the colony-forming cells have proliferative properties in common with CFU-S and respond to specific proliferation regulators previously detected using the spleen colony assay.
Pragnell
I B
IB
Beatson Institute for Cancer Research, Wolfson Laboratory, Glasgow, U.K.
Wright
E G
EG
Lorimore
S A
SA
Adam
J
J
Rosendaal
M
M
DeLamarter
J F
JF
Freshney
M
M
Eckmann
L
L
Sproul
A
A
Wilkie
N
N
D000818:Animals; D001854:Bone Marrow Cells; D002455:Cell Division / Q000187:drug effects; D003114:Colony-Forming Units Assay; D005260:Female; D005472:Fluorouracil / Q000633:toxicity; D006133:Growth Substances / Q000145:classification / Q000502:physiology*; D006410:Hematopoiesis / Q000187:drug effects; D016298:Hematopoietic Cell Growth Factors; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D013154:Spleen / Q000166:cytology
D006133:Growth Substances; D016298:Hematopoietic Cell Growth Factors; D005472:Fluorouracil