Identification of a novel monocyte cell surface molecule involved in the generation of antigen-induced proliferative responses.
A monocyte molecule, identified by a monoclonal antibody (mAb) named 24, is involved in the antigen-specific proliferation of T cells. Several types of antigen-induced responses are blocked by mAb24, but mitogen responses, even at low doses, are not. The 24 molecule is found on circulating monocytes and no other cell type. Normally only a proportion of monocytes express the 24 molecule but it can be induced rapidly on monocytes in culture, possibly from intracellular sources. The epitope is present on a heterodimer of 175 and 95 kDa which is similar to the leukocyte cell adhesion molecule (Leu-CAM) family. These findings suggest that although the 24 molecule shows some functional and biochemical similarities with the Leu-CAM family of molecules, it represents a novel structure primarily associated with mononuclear phagocytes.
Dougherty
G J
GJ
Macrophage Laboratory, Imperial Cancer Research Fund, London, GB.
Dransfield
I
I
Hogg
N
N
D000911:Antibodies, Monoclonal / Q000276:immunology*; D000937:Antigen-Antibody Reactions; D000938:Antigen-Presenting Cells / Q000276:immunology; D015214:Antigens, Differentiation, Myelomonocytic / Q000276:immunology*; D000956:Antigens, Viral / Q000276:immunology; D005434:Flow Cytometry; D006801:Humans; D066298:In Vitro Techniques; D008213:Lymphocyte Activation; D009000:Monocytes / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology; D014373:Tuberculin / Q000276:immunology
D000911:Antibodies, Monoclonal; D015214:Antigens, Differentiation, Myelomonocytic; D000956:Antigens, Viral; D014373:Tuberculin