A competitive inhibition enzyme immunoassay for detection and quantification of organophosphorus compounds.
A sensitive and specific method for detection and quantification of methyl phosphonic acid, p-aminophenyl 1,2,2-trimethyl-propyl diester (MATP) as a model substance for organophosphorus compounds is described. Different procedures for coupling the haptenic group for immunization, purification and immobilization allowed the detection of hapten-specific antibodies. The competitive inhibition enzyme immunoassay (CIEIA), using purified chicken and rabbit IgG-antibodies, was able to detect MATP-concentrations as low as 10(-10) mol/l. Based upon our results, we postulate that the CIEIA represents a good alternative to the customary diagnosis of organophosphate intoxications, measuring blood cholinesterase activity.
Schmidt
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P
Institut für Physiologie, Physiologische Chemie und Ernährungsphysiologie, Tierärztliche Fakultät, Universität München, Bundesrepublik Deutschland.
Kühlmann
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R
Lösch
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U
D000818:Animals; D000906:Antibodies / Q000302:isolation & purification; D002645:Chickens / Q000276:immunology; D007124:Immunoenzyme Techniques; D007202:Indicators and Reagents; D009943:Organophosphorus Compounds / Q000032:analysis*; D011817:Rabbits / Q000276:immunology
D000906:Antibodies; D007202:Indicators and Reagents; D009943:Organophosphorus Compounds; C056499:4-aminophenylphosphonic acid methyl-1,2,2-trimethylpropyl diester