[Immunoenzyme test system based on antibody F(ab)2-fragments for detecting the NP protein of the influenza virus].
A test system has been proposed for the detection of influenza A virus NP protein by solid-phase enzyme immunoassay in a modified system of "double" antibodies: the primary antibodies were F(ab)2-fragments of immunoglobulin class G isolated from rabbit antiserum specific for NP protein which was also used as the source of secondary antibodies. The antigen-bound antibodies were detected by protein A (from St. aureus) conjugate with horseradish peroxidase. The high specificity and sensitivity of the test system (0.3 ng/ml NP protein) and a minimal level of nonspecific reactions were demonstrated. The advantages of this method are discussed.
Leonov
S V
SV
Khristova
M L
ML
Sokolova
M V
MV
Dönner
L
L
Kharitonenkov
I G
IG
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology* / Q000302:isolation & purification; D000956:Antigens, Viral / Q000032:analysis; D004306:Dose-Response Relationship, Immunologic; D007124:Immunoenzyme Techniques / Q000295:instrumentation; D007140:Immunoglobulin Fab Fragments / Q000276:immunology* / Q000302:isolation & purification; D009980:Influenza A virus / Q000276:immunology*; D009981:Influenza B virus / Q000276:immunology*; D019590:Nucleocapsid Proteins; D009698:Nucleoproteins; D011817:Rabbits; D014758:Viral Core Proteins / Q000032:analysis*
D000914:Antibodies, Viral; D000956:Antigens, Viral; D007140:Immunoglobulin Fab Fragments; D019590:Nucleocapsid Proteins; D009698:Nucleoproteins; D014758:Viral Core Proteins
Immunofermentnaia test-sistema na osnove F(ab)2-fragmentov antitel dlia vyiavleniia belka NP virusa grippa.