The distribution of two hnRNP-associated proteins defined by a monoclonal antibody is altered in heat-shocked HeLa cells.
A monoclonal antibody obtained after mice were immunized with hnRNP purified from HeLa cells recognizes two polypeptides of Mr 35,000 and 37,000. By immunocytofluorescence, these antigens can be visualized only in cells previously heat shocked at 45 degrees C for 5 or 10 min, although they are present at the same level in unstressed and stressed cells. The signal, which is mostly concentrated in the interchromatin space, where hnRNP fibrils are located, does not accumulate with time and disappears 4 to 5 h after heat shock. Discrimination between the two types of hnRNP substructures, the 30-50 S monoparticles and the nuclear matrix fibrils, based on differential sensitivity to salt or ribonuclease treatment, showed that in unstressed cells the antigens behave as monoparticle proteins. In contrast, in heat-shocked cells, most 35-37K antigens behave as nuclear matrix proteins. Thus, heat shock seems to induce a rapid and reversible switch of these two antigens from hnRNP monoparticles to the nuclear matrix. The data demonstrate that heat shock, which was previously shown not to alter the overall RNA: protein packaging ratio of hnRNP, induces subtle modifications of their substructure. Such modifications might be of importance since heat shock is known for instance to affect pre-mRNA processing.
Lutz
Y
Y
Laboratoire de Génétique Moléculaire des Eucaryotes du CNRS, Strasbourg, France.
Jacob
M
M
Fuchs
J P
JP
D000911:Antibodies, Monoclonal / Q000276:immunology*; D002451:Cell Compartmentation; D002467:Cell Nucleus / Q000378:metabolism / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D006367:HeLa Cells; D034441:Heterogeneous-Nuclear Ribonucleoproteins; D006358:Hot Temperature; D006801:Humans; D008970:Molecular Weight; D012261:Ribonucleoproteins / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D034441:Heterogeneous-Nuclear Ribonucleoproteins; D012261:Ribonucleoproteins