[Determining the level of the rheumatoid factor associated with polyclonal and monoclonal human serum IgA].
The authors studied the activity of the rheumatoid factor (RF) associated with polyclonal and monoclonal IgA contained in the sera of patients with chronic diseases of noninfectious etiology and A-paraproteinemia. IgA-RF-activity was determined by an enzyme immunoassay. In most of the patients' sera IgA had a high level of RF-activity. The frequency of RF-activity detection did not depend on the level of polyclonal IgA but correlated with the level of monoclonal serum IgA. During a study of the chromatographic fractions of sera containing monoclonal IgA, RF-activity was mainly revealed in the fraction of IgA polymers; IgA monomers forming the bulk of serum IgA, demonstrated no RF-activity. The inclination of curves reflecting the correlation between the level of IgA-RF and dilutions of tested sera made it possible to assess function of the affinity of such molecules which was growing with an increase in IgA molecular mass.
German
G P
GP
Varlamova
E Iu
EIu
Andreeva
N E
NE
D000911:Antibodies, Monoclonal / Q000032:analysis*; D000915:Antibody Affinity; D002850:Chromatography, Gel; D002908:Chronic Disease; D005922:Glomerulonephritis, IGA / Q000276:immunology*; D006801:Humans; D007124:Immunoenzyme Techniques; D007070:Immunoglobulin A / Q000032:analysis*; D010265:Paraproteinemias / Q000276:immunology*; D012217:Rheumatoid Factor / Q000032:analysis*
D000911:Antibodies, Monoclonal; D007070:Immunoglobulin A; D012217:Rheumatoid Factor
Opredelenie revmatoidnogo faktora, sviazannogo s poliklonal'nym i monoklonal'nym syvorotochnym IgA cheloveka.