title: Modulating activity of B lymphocytes on haemopoiesis (II)--the purification of proliferation amplifying factor (PAF).
abstract: In recent years we have done a series of observations on the haemopoietic modulation effects of lymphocytes. Now we report here, for the first time, the purification of a modulating factor (proliferation amplifying factor, PAE) with affinity chromatography and HPLC from supernatant conditioned by a lymphoma cell line M12.4.1. RAF exhibits on SDS-PAGE only one band, the molecular weight is about 31,000, the specific activity is 20,000 times that in the conditioned medium. It may provide a direct evidence that the B lymphocyte also possesses some haemopoietic modulation effects.
authors.affiliation: Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing.
authors.forename: Z J
authors.initials: ZJ
authors.lastname: Yao
authors.forename: F Z
authors.initials: FZ
authors.lastname: Jiang
authors.forename: Y
authors.initials: Y
authors.lastname: Li
authors.forename: Y J
authors.initials: YJ
authors.lastname: Guo
authors.forename: M W
authors.initials: MW
authors.lastname: Zhang
authors.forename: Y J
authors.initials: YJ
authors.lastname: Xu
authors.forename: P X
authors.initials: PX
authors.lastname: Tang
mesh_terms: D001402:B-Lymphocytes / Q000502:physiology*; D003114:Colony-Forming Units Assay; D006410:Hematopoiesis; D006801:Humans; D008223:Lymphoma / Q000032:analysis; D011506:Proteins / Q000302:isolation & purification*; D014407:Tumor Cells, Cultured
chemical_list: D011506:Proteins