title: Gene mapping in marsupials and monotremes, V. Synteny between hypoxanthine phosphoribosyltransferase and phosphoglycerate kinase in the platypus.
abstract: In order to extend comparative mapping studies to the monotreme mammals (subclass Prototheria), somatic-cell hybrids were obtained between Chinese-hamster cells deficient in hypoxanthine phosphoribosyltransferase (HPRT) and platypus fibroblasts. The characteristics of these hybrids closely resemble those of metatherian x eutherian hybrids, in that they are recovered at low frequency and they rapidly segregate and fragment platypus chromosomes. Biochemical and cytological studies of the hybrids, their subclones and HPRT-deficient revertants indicate that phosphoglycerate kinase is syntenic with HPRT in the platypus (as it is in other mammals); however, the studies do not permit chromosomal assignment of the syntenic group. The implications of the chromosomal location of this ancient synteny group for the evolution of the mammalian X chromosome are discussed.
authors.affiliation: Department of Genetics and Human Variation, La Trobe University, Bundoora.
authors.forename: J M
authors.initials: JM
authors.lastname: Watson
authors.forename: J A
authors.initials: JA
authors.lastname: Graves
mesh_terms: D000818:Animals; D005075:Biological Evolution; D002871:Chromosome Banding; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D006224:Cricetinae; D005347:Fibroblasts / Q000201:enzymology; D006822:Hybrid Cells / Q000201:enzymology; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics*; D007621:Karyotyping; D009007:Monotremata / Q000235:genetics*; D010735:Phosphoglycerate Kinase / Q000235:genetics*; D010987:Platypus / Q000235:genetics*
chemical_list: D007041:Hypoxanthine Phosphoribosyltransferase; D010735:Phosphoglycerate Kinase