Immunoblotting and the immune response to leprosy.
Immunoblotting has provided a powerful and effective approach to dissection of the immune response to mycobacterial antigens in a situation in which the availability of isolated antigenic components is severely limited. The basic approach of blotting on to a solid-phase support has been used in combination with SDS/polyacrylamide-gel electrophoresis, t.l.c., recombinant DNA technology and T-cell cloning in order to carry out a comprehensive analysis of glycolipid and protein antigens involved in the immune response to mycobacterial infection.
Young
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M.R.C. Tuberculosis and Related Infections Unit, Hammersmith Hospital, London, U.K.
D000942:Antigens, Bacterial / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D006017:Glycolipids / Q000032:analysis*; D006801:Humans; D007118:Immunoassay / Q000379:methods; D007918:Leprosy / Q000276:immunology*; D009166:Mycobacterium leprae / Q000276:immunology*
D000942:Antigens, Bacterial; D006017:Glycolipids; C045169:phenolic glycolipid I, Mycobacterium leprae