Effects of epidermal growth factor and insulin-like growth factor I on the levels of mRNA encoding aromatase cytochrome P-450 of human ovarian granulosa cells.
The effects of growth factors to regulate the activity of aromatase, as well as the synthesis of aromatase cytochrome P-450 (P-450AROM) have been studied in human ovarian granulosa cells obtained from women undergoing oocyte retrieval. Insulin-like growth factor I (IGF-I) increased aromatase activity as well as the synthesis of P-450AROM, in a concentration-dependent fashion. The levels of hybridizable mRNA species encoding cytochrome P-450AROM were also increased with IGF-I treatment. By contrast, epidermal growth factor (EGF) had no effect on these parameters when added alone, but markedly inhibited the action of follicle-stimulating hormone (FSH) to stimulate aromatase activity, and the synthesis of cytochrome P-450AROM, as well as its ability to increase the levels of mRNA encoding the enzyme. It is concluded that these growth factors have opposite effects on aromatase activity, and that these actions reflect, in part, changes in the synthesis of cytochrome P-450AROM, which in turn are the consequence of changes in the levels of mRNA encoding this enzyme.
Steinkampf
M P
MP
Department of Obstetrics and Gynecology, University of Texas Southwestern Medical Center, Dallas 75235-9051.
Mendelson
C R
CR
Simpson
E R
ER
D001141:Aromatase / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D002478:Cells, Cultured; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D005260:Female; D005815:Genetic Code; D006107:Granulosa Cells / Q000032:analysis / Q000187:drug effects* / Q000201:enzymology; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D010053:Ovary / Q000032:analysis / Q000187:drug effects* / Q000201:enzymology; D012333:RNA, Messenger / Q000032:analysis / Q000187:drug effects* / Q000235:genetics; D013002:Somatomedins / Q000494:pharmacology*
D012333:RNA, Messenger; D013002:Somatomedins; D004815:Epidermal Growth Factor; D007334:Insulin-Like Growth Factor I; D001141:Aromatase