Use of a cross reacting anti-idiotype to identify normal counterparts of neoplastic cells.
We have established that 9G4, a cross reacting monoclonal anti-idiotype identifies an epitope close the binding site of anti-I cold agglutinins. 9G4 positive lymphocytes comprise around 5% of B cells and are widely represented in all lymphoid tissue. They may be detected in 15 week fetal spleen at the time of the earliest appearance of B cells and thus form part of the basic B cell repertoire. Such antibodies are derived largely from CD5 positive B cells. 9G4 is thus a useful tool to investigate the normal counterpart of the CLL cell.
Stevenson
F K
FK
Tenovus Research Laboratory, General Hospital, Southampton, UK.
Smith
G J
GJ
North
J
J
Glennie
M G
MG
Hamblin
T J
TJ
D000328:Adult; D000373:Agglutinins / Q000032:analysis; D000888:Antibodies, Anti-Idiotypic / Q000502:physiology; D000911:Antibodies, Monoclonal / Q000502:physiology; D001402:B-Lymphocytes / Q000032:analysis*; D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D003429:Cross Reactions; D003450:Cryoglobulins; D006801:Humans; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000276:immunology*
D000373:Agglutinins; D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D003450:Cryoglobulins; D007130:Immunoglobulin Idiotypes; C029443:cold agglutinins