title: Characterization of recombinant human prorenin and renin.
abstract: A cell line that secretes substantial quantities of recombinant human prorenin was prepared by transfecting Chinese hamster ovary cells with a gene encoding preprorenin. The prorenin was purified to homogeneity and was found to have a single amino terminus, reflecting cleavage after a typical 23 amino acid signal sequence. The purified inactive prorenin was not a substrate for active renin and was not capable of self-activation. Prorenin could be converted to renin by addition of exogenous protease, and deglycosylation of the prorenin did not alter the sensitivity to protease activation. The enzymatic activity of deglycosylated renin was kinetically identical to that of the native protein. Multimilligram quantities of recombinant human renin and prorenin were purified, providing suitable material for studies directed toward greater understanding of the function of these proteins and for structural studies such as x-ray diffraction for use in design of renin inhibitors.
authors.affiliation: California Biotechnology, Inc., Mountain View 94043.
authors.forename: C T
authors.initials: CT
authors.lastname: Carilli
authors.forename: J L
authors.initials: JL
authors.lastname: Vigne
authors.forename: L C
authors.initials: LC
authors.lastname: Wallace
authors.forename: L M
authors.initials: LM
authors.lastname: Smith
authors.forename: M A
authors.initials: MA
authors.lastname: Wong
authors.forename: J A
authors.initials: JA
authors.lastname: Lewicki
authors.forename: J D
authors.initials: JD
authors.lastname: Baxter
mesh_terms: D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D004792:Enzyme Precursors / Q000302:isolation & purification* / Q000378:metabolism; D005260:Female; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D010053:Ovary; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism; D012083:Renin / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D004792:Enzyme Precursors; D011994:Recombinant Proteins; D012083:Renin