title: Detection and purification of a recombinant human B lymphotropic virus (HHV-6) in the baculovirus expression system by limiting dilution and DNA dot-blot hybridization.
abstract: A recombinant virus Ac373-HB was detected and purified from the transfection mixture of wild type and recombinant virus in the baculovirus expression system using a combination of limiting dilution and DNA dot-blot hybridization. This method allows for a quick and convenient way of detection and purification of recombinant virus without the need to use a plaque purification step. It is generally applicable to other expression systems besides the baculovirus system described here.
authors.affiliation: Department of Virology and Epidemiology, Baylor College of Medicine, Houston, Texas 77030.
authors.forename: M C
authors.initials: MC
authors.lastname: Fung
authors.forename: K Y
authors.initials: KY
authors.lastname: Chiu
authors.forename: T
authors.initials: T
authors.lastname: Weber
authors.forename: T W
authors.initials: TW
authors.lastname: Chang
authors.forename: N T
authors.initials: NT
authors.lastname: Chang
mesh_terms: D001402:B-Lymphocytes / Q000382:microbiology*; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D005822:Genetic Vectors; D006564:Herpesviridae / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D007304:Insect Viruses / Q000235:genetics; D008828:Microbiological Techniques; D009693:Nucleic Acid Hybridization; D011995:Recombination, Genetic
chemical_list: D004279:DNA, Viral