title: Immunocytochemical identification of bovine Langerhans cells by use of a monoclonal antibody directed against class II MHC antigens.
abstract: We undertook a study to develop a reliable light microscopic technique for identifying Langerhans cells (LC) in bovine epidermis. Monoclonal antibodies (MCA) detecting bovine class II MHC antigens were used in conjunction with an avidin-biotin-peroxidase complex (ABC) immunocytochemical staining method. The specificity of the MCA for LC was confirmed ultrastructurally by use of gold-labeled second antibody. Epidermal sheets and epidermal single-cell suspensions examined by light microscopy confirm that bovine epidermal LC express class II antigens. Anti-bovine class II MCA is a dependable reagent for identification of LC in normal bovine epidermis.
authors.affiliation: Department of Veterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Canada.
authors.forename: L A
authors.initials: LA
authors.lastname: Bryan
authors.forename: P J
authors.initials: PJ
authors.lastname: Griebel
authors.forename: D M
authors.initials: DM
authors.lastname: Haines
authors.forename: W C
authors.initials: WC
authors.lastname: Davis
authors.forename: J R
authors.initials: JR
authors.lastname: Allen
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D002417:Cattle; D000078404:Epidermal Cells; D006649:Histocompatibility Antigens / Q000032:analysis* / Q000276:immunology; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D007801:Langerhans Cells / Q000166:cytology* / Q000276:immunology; D008854:Microscopy, Electron
chemical_list: D000911:Antibodies, Monoclonal; D006649:Histocompatibility Antigens