[Methods of determining the activity of proteolytic enzymes].
The present methods for determination of the proteinases activity based on the protein, low-molecular weight, fluorescent and radioactive substrates, also active-site titration have been analyzed. The comparative evaluation of its effectiveness which depends on duration, pH range, possible errors, caused by concomitant proteinases are considered. The influence of this factors on the choice of assay for measuring the proteinases activity and its efficiency are discussed.
Borodinskaia
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Mishunin
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D008722:Methods; D010447:Peptide Hydrolases / Q000032:analysis*; D013379:Substrate Specificity
D010447:Peptide Hydrolases
Metody opredeleniia aktivnosti proteoliticheskikh fermentov.