The yeast SNF3 gene encodes a glucose transporter homologous to the mammalian protein.
The SNF3 gene is required for high-affinity glucose transport in the yeast Saccharomyces cerevisiae and has also been implicated in control of gene expression by glucose repression. We report here the nucleotide sequence of the cloned SNF3 gene. The predicted amino acid sequence shows that SNF3 encodes a 97-kilodalton protein that is homologous to mammalian glucose transporters and has 12 putative membrane-spanning regions. We also show that a functional SNF3-lacZ gene-fusion product cofractionates with membrane proteins and is localized to the cell surface, as judged by indirect immunofluorescence microscopy. Expression of the fusion protein is regulated by glucose repression.
Celenza
J L
JL
Department of Genetics, Columbia University College of Physicians and Surgeons, New York 10032.
Marshall-Carlson
L
L
Carlson
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005656:Fungal Proteins / Q000235:genetics*; D005786:Gene Expression Regulation / Q000187:drug effects; D005796:Genes; D005800:Genes, Fungal; D005947:Glucose / Q000494:pharmacology; D006801:Humans; D008969:Molecular Sequence Data; D009004:Monosaccharide Transport Proteins / Q000235:genetics*; D051381:Rats / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
D005656:Fungal Proteins; D009004:Monosaccharide Transport Proteins; D011993:Recombinant Fusion Proteins; D005947:Glucose