Preparation of raf-oncogene-specific antiserum with raf protein produced in E. coli.
In this report we describe the expression of v-raf protein in E. coli using a tryptophan-promoter-driven expression vector and its immunological characterization by anti-peptide sera. The purified recombinant protein was used to produce raf-specific antibodies which are suitable for studying v-raf and c-raf proteins in vitro and in vivo in a variety of species ranging from mouse to man.
Kolch
W
W
Laboratory of Viral Carcinogenesis, NCI-Frederick Cancer Research Facility, MD 21701.
Schultz
A M
AM
Oppermann
H
H
Rapp
U R
UR
D000595:Amino Acid Sequence; D000918:Antibody Specificity; D011232:Chemical Precipitation; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D008970:Molecular Weight; D009857:Oncogenes; D010446:Peptide Fragments / Q000276:immunology; D011994:Recombinant Proteins / Q000276:immunology*; D013329:Structure-Activity Relationship
D010446:Peptide Fragments; D011994:Recombinant Proteins