title: Development of insulin and epidermal growth factor receptors during the differentiation of rat preadipocytes in primary culture.
abstract: An homogeneous cell population isolated from the inguinal tissue of 3-day-old rats is able to proliferate in primary culture. In the presence of a physiological concentration of insulin (1.5 nM) it converts into cells exhibiting the morphology and the biochemical characteristics of adipocytes. Insulin and epidermal growth factor (EGF) receptors were studied during both the exponential growth and the adipose conversion phases of these cells. Binding experiments with 125I-labelled peptides were performed directly in the culture dishes. The number of high affinity insulin binding sites increased, during the entire culture period studied, reaching 18 days after plating the value of 10,600 x 2360. Control cells (cultured in the presence of anti-insulin antibody) exhibited an increase of the concentration of insulin binding sites from no more than 500 sites/cell to 6880 +/- 1710 sites/cell between dat 0 and 9 (corresponding to the exponential growth phase); this increase was followed by a rapid reduction in insulin receptors during the stationary phase. The density of EGF binding sites increased between day 0 and 4 (one cell cycle), whether the cells were maintained or not with insulin, and plateaued thereafter. Mature adipocytes freshly isolated from the inguinal tissue of 3-day-old rats had no detectable EGF binding sites, but their content in high affinity binding sites for insulin was similar to that of cells after complete adipocyte conversion in primary culture.
authors.affiliation: INSERM U.55, Centre de Recherches de l'Hôpital Saint-Antoine, Paris, France.
authors.forename: A M
authors.initials: AM
authors.lastname: Gaben-Cogneville
authors.forename: B
authors.initials: B
authors.lastname: Poussin
authors.forename: M C
authors.initials: MC
authors.lastname: Chamblier
authors.forename: M E
authors.initials: ME
authors.lastname: Forgue-Lafitte
authors.forename: G
authors.initials: G
authors.lastname: Rosselin
mesh_terms: D000273:Adipose Tissue / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D004815:Epidermal Growth Factor / Q000378:metabolism; D066246:ErbB Receptors / Q000096:biosynthesis*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D007328:Insulin / Q000378:metabolism / Q000494:pharmacology; D008297:Male; D051381:Rats; D011972:Receptor, Insulin / Q000096:biosynthesis*
chemical_list: D007328:Insulin; D004815:Epidermal Growth Factor; D066246:ErbB Receptors; D011972:Receptor, Insulin