title: Optimal conditions for recovery of the human immunodeficiency virus from peripheral blood mononuclear cells.
abstract: Optimal conditions for demonstrating the presence of infectious human immunodeficiency virus in peripheral blood mononuclear cells (PMCs) from seropositive individuals involved cocultivation of infected cells with phytohemagglutinin-stimulated PMCs from seronegative donors in the presence of 2 micrograms of Polybrene per ml. The size of the culture vessel also influenced the results; smaller numbers of infected cells were detected under conditions of increased cell density. In addition, an increased normal donor/patient PMC ratio was helpful. The cocultivation approach permitted identification of human immunodeficiency virus in over 90% of seropositive individuals with different clinical conditions. Moreover, reconstruction experiments indicated that this method allows detection of one productively infected CD4+ cell in a population of over 10(6) PMCs.
authors.affiliation: Cancer Research Institute, University of California, San Francisco 94143.
authors.forename: B A
authors.initials: BA
authors.lastname: Castro
authors.forename: C D
authors.initials: CD
authors.lastname: Weiss
authors.forename: L D
authors.initials: LD
authors.lastname: Wiviott
authors.forename: J A
authors.initials: JA
authors.lastname: Levy
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000097:blood* / Q000382:microbiology; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D006678:HIV / Q000302:isolation & purification*; D006679:HIV Seropositivity; D006801:Humans; D009000:Monocytes / Q000382:microbiology*; D012016:Reference Values
chemical_list: D000945:Antigens, Differentiation, T-Lymphocyte