title: Detection of a low frequency of activated ras genes in human melanomas using a tumorigenicity assay.
abstract: We have used an assay combining DNA-mediated gene transfer and tumorigenicity in Swiss athymic mice to look for activated ras genes in solid human sporadic melanomas. This assay can detect ras oncogenes mutated at codons 12, 13, or 61. We examined a panel of 13 independent surgical specimens of primary tumors and metastases. No H- or K-ras oncogenes were detected; an N-ras oncogene, mutated at codon 61, was identified in one of the 13 samples. No N-ras genes mutated at codon 13 were detected. Thus, the tumorigenicity assay detects a low frequency of ras gene activation in melanomas.
authors.affiliation: Unité 119 de l'INSERM, Marseille, France.
authors.forename: F
authors.initials: F
authors.lastname: Raybaud
authors.forename: T
authors.initials: T
authors.lastname: Noguchi
authors.forename: I
authors.initials: I
authors.lastname: Marics
authors.forename: J
authors.initials: J
authors.lastname: Adelaide
authors.forename: J
authors.initials: J
authors.lastname: Planche
authors.forename: M
authors.initials: M
authors.lastname: Batoz
authors.forename: C
authors.initials: C
authors.lastname: Aubert
authors.forename: O
authors.initials: O
authors.lastname: de Lapeyriere
authors.forename: D
authors.initials: D
authors.lastname: Birnbaum
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D004273:DNA, Neoplasm / Q000235:genetics*; D005091:Exons; D011905:Genes, ras; D006801:Humans; D008545:Melanoma / Q000235:genetics* / Q000473:pathology; D051379:Mice; D008969:Molecular Sequence Data; D009857:Oncogenes
chemical_list: D004273:DNA, Neoplasm