title: [Expression of gag gene of human immunodeficiency virus in recombinant vaccinia virus].
abstract: A fragment of HTLV-IIIB gag-gene, coding for the first 441 amino acids of the p53 gag-precursor was expressed in the recombinant vaccinia virus, vC5. Two HIV specific proteins were detected by western blot in CV-1 cells infected with vC5. Their relative molecular masses were 50 and 35 Kd, pointing out that the first of the proteins is a full length expression product of the cloned sequence, while the second one is a result of processing or abortive translation. Possibilities of using such a strain as a vaccine or in Western blot conformation test are discussed.
authors.forename: S A
authors.initials: SA
authors.lastname: Popov
authors.forename: E V
authors.initials: EV
authors.lastname: Barsov
authors.forename: M I
authors.initials: MI
authors.lastname: Bukrinskiĭ
authors.forename: V M
authors.initials: VM
authors.lastname: Zhdanov
mesh_terms: D005786:Gene Expression Regulation; D015683:Gene Products, gag; D005814:Genes, Viral; D006678:HIV / Q000235:genetics* / Q000276:immunology; D015488:HIV Antigens / Q000235:genetics; D010957:Plasmids; D011995:Recombination, Genetic; D012191:Retroviridae Proteins / Q000235:genetics*; D014616:Vaccinia virus / Q000235:genetics*
chemical_list: D015683:Gene Products, gag; D015488:HIV Antigens; D012191:Retroviridae Proteins
vernacular_title: Ekspressiia gena gag virusa immunodefitsita cheloveka v sostave rekombinantnogo virusa ospovaktsiny.