title: Efficient expression of the yeast metallothionein gene in Escherichia coli.
abstract: The yeast metallothionein gene CUP1 was cloned into a bacterial expression system to achieve efficient, controlled expression of the stable, unprocessed protein product. The Escherichia coli-synthesized yeast metallothionein bound copper, cadmium, and zinc, indicating that the protein was functional. Furthermore, E. coli cells expressing CUP1 acquired a new, inducible ability to selectively sequester heavy metal ions from the growth medium.
authors.affiliation: Department of Molecular Genetics, Smith Kline & French Laboratories, Swedeland, Pennsylvania 19406.
authors.forename: T
authors.initials: T
authors.lastname: Berka
authors.forename: A
authors.initials: A
authors.lastname: Shatzman
authors.forename: J
authors.initials: J
authors.lastname: Zimmerman
authors.forename: J
authors.initials: J
authors.lastname: Strickler
authors.forename: M
authors.initials: M
authors.lastname: Rosenberg
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002104:Cadmium / Q000378:metabolism; D003001:Cloning, Molecular; D003300:Copper / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation; D005796:Genes; D005798:Genes, Bacterial; D005800:Genes, Fungal; D008668:Metallothionein / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D015032:Zinc / Q000378:metabolism
chemical_list: D002104:Cadmium; D003300:Copper; D008668:Metallothionein; D015032:Zinc