[Effect of insulin on cartilage differentiation in vitro].
A consistent chondrogenesis takes place in high-density microcultures derived from bud mesenchymal cells of 4-day-old chicken embryos in a serum-supplemented medium. In serum-free medium DNA level and uronic acid content in the cultures were low, as well as the 35SO4 uptake and release, and only a small mass of cartilage was formed. With the addition of 0.025-10 micrograms/ml insulin to serum-free medium the uronic acid and DNA content in the cultures increased considerably in a dose-dependent way. The intensity of 35SO4 uptake and release exceeded the values measured in serum-containing medium, more cartilage tissue was formed in them also in a dose-dependent manner. With the use of 20-80 micrograms/ml insulin, the increment in DNA content proved to decrease, and with the use of 80 micrograms/ml insulin the uronic acid content and the cartilage mass decreased to a greater extent than in the case of lover doses.
Hadházy
C s
Cs
Dedukh
N V
NV
D000818:Animals; D002356:Cartilage / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D002642:Chick Embryo; D046508:Culture Techniques; D004247:DNA / Q000096:biosynthesis / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D007328:Insulin / Q000494:pharmacology*; D013431:Sulfates / Q000378:metabolism; D013997:Time Factors; D014574:Uronic Acids / Q000378:metabolism
D007328:Insulin; D013431:Sulfates; D014574:Uronic Acids; D004247:DNA
Vliianie insulina na differentsirovku khriashcha in vitro.