An immunogold cell labelling method for rat T lymphocytes.
A method using monoclonal antibodies in conjunction with an immunogold procedure was adapted for labelling T lymphocytes in blood samples obtained from male Wistar rats. The monoclonal antibodies W3/25, MRC/OX8 and W3/13 were used. Changes in the percentages of positively labelled cells were observed in rats dosed with the immunosuppressant cyclosporin.
Evans
G O
GO
Department of Toxicology and Metabolism, Wellcome Research Laboratories, Beckenham, Kent, UK.
Flynn
R M
RM
Lupton
J D
JD
D000818:Animals; D000911:Antibodies, Monoclonal; D003524:Cyclosporins / Q000494:pharmacology; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D051381:Rats; D011919:Rats, Inbred Strains / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
D000911:Antibodies, Monoclonal; D003524:Cyclosporins