title: A modified assay for cyclosporin in blood using solid-phase extraction with high-performance liquid chromatography.
abstract: A modified procedure for measuring cyclosporin in whole blood by high-performance liquid chromatography is described and evaluated for clinical use. Sample preparation uses solid-phase extraction cartridges that can be reused. Life of the reverse-phase analytical column exceeds 1,000 injections at 70 degrees C. Cyclosporins A and D (internal standard) elute after 5.6 and 7.6 min, respectively. Calibration plots are linear from 50 ng/ml to at least 2,000 ng/ml. Within-day and between-day imprecision is less than 9% (coefficient of variation). Minimum measurable concentration is 50 ng/ml.
authors.affiliation: Department of Clinical Pharmacology, Princess Alexandra Hospital, Brisbane, Queensland, Australia.
authors.forename: B G
authors.initials: BG
authors.lastname: Charles
authors.forename: R L
authors.initials: RL
authors.lastname: Norris
authors.forename: P J
authors.initials: PJ
authors.lastname: Ravenscroft
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation; D002986:Clinical Trials as Topic; D003524:Cyclosporins / Q000097:blood*; D006801:Humans; D008991:Monitoring, Physiologic
chemical_list: D003524:Cyclosporins