Activation of c-Ki-ras coexists with c-myc amplification in cells from a nude mouse tumor induced by the human breast carcinoma cell line SW 613-S.
In vitro transfection experiments have shown that cooperation between two different oncogenes can confer a fully malignant phenotype to primary rodent cells. We have previously reported that SW 613-Tul cells, derived from a tumor induced in a nude mouse by the human breast carcinoma cell line SW 613-S, showed a 30-fold amplification of the c-myc gene. In the present work, we show that these cells also harbor an activated c-Ki-ras gene capable of inducing the formation of foci upon transfection of NIH 3T3 cells with SW 613-Tul genomic DNA. Our results suggest that both the c-myc and c-Ki-ras oncogenes, activated by two different mechanisms, may cooperate in the full expression of the tumorigenic phenotype of SW 613-Tul cells.
Carloni
G
G
Laboratoire de Génétique Cellulaire, ER 278, CNRS, Institut de Recherches Scientifiques sur le Cancer, Villejuif, France.
Champ
B
B
Vilarem
M J
MJ
Lavialle
C
C
Cassingena
R
R
D000818:Animals; D001943:Breast Neoplasms / Q000235:genetics*; D002460:Cell Line; D002478:Cells, Cultured; D004273:DNA, Neoplasm / Q000235:genetics; D005260:Female; D005784:Gene Amplification; D005786:Gene Expression Regulation; D011905:Genes, ras; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009693:Nucleic Acid Hybridization; D011519:Proto-Oncogenes; D014162:Transfection
D004273:DNA, Neoplasm