Histamine H1-receptor in endothelial and smooth muscle cells of guinea-pig aorta.
The location of histamine H1-receptors in the thoracic aorta of guinea-pigs was studied with a [3H]mepyramine binding assay. [3H]Mepyramine binding studies of whole and rubbed aortas, and of cultured endothelial and smooth muscle cells showed that the Kd values were all in the range 0.53-0.76 nM, but that the Bmax values were 19.1, 10.1, 63.3 and 11.6 fmol/mg protein, respectively. Thus, the whole aorta contained more H1-receptors than the rubbed one (free of endothelium), and cultured endothelial cells contained more H1-receptors than smooth muscle cells. These results indicate that more histamine H1-receptors were concentrated in the endothelial cells than in the smooth muscle cells in guinea-pig aorta.
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Department of Dermatology, Hiroshima University School of Medicine, Japan.
Fukui
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Watanabe
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Wada
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Yamamoto
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D000818:Animals; D001013:Aorta, Thoracic / Q000032:analysis*; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000032:analysis* / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D006168:Guinea Pigs; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000032:analysis* / Q000648:ultrastructure; D011738:Pyrilamine / Q000378:metabolism; D011968:Receptors, Histamine / Q000032:analysis*; D011969:Receptors, Histamine H1 / Q000032:analysis*; D014316:Tritium
D011968:Receptors, Histamine; D011969:Receptors, Histamine H1; D014316:Tritium; D011738:Pyrilamine