Yeast cell debris and protein partitioning in the poly(ethylene glycol)-poly(vinyl alcohol) biphasic system.
Yeast cells, cell debris and protein partitioning have been investigated in the poly(ethylene glycol) (PEG) 8000/poly(vinyl alcohol) (PVA) 10,000 system. Cells and cell debris partition into the lower (PVA) phase over the pH range 4.8-7.5, and with up to 0.37 M KCl at pH 5.9. Protein partitioning is more pH-dependent in the PEG/PVA system than in the PEG/dextran system, and a significant fraction of the total protein is found at the interface at lower pH values. Significant, rapid purification of overproduced pyruvate kinase in a PEG/PVA system containing Blue Sepharose CL-6B particles is demonstrated.
Kokkoris
A
A
Department of Chemical Engineering, West Virginia University, Morgantown 26506.
Blair
J B
JB
Shaeiwitz
J A
JA
D002458:Cell Fractionation / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D011092:Polyethylene Glycols; D011142:Polyvinyl Alcohol; D011506:Proteins / Q000302:isolation & purification; D011770:Pyruvate Kinase / Q000302:isolation & purification; D012441:Saccharomyces cerevisiae / Q000032:analysis*
D011506:Proteins; D011092:Polyethylene Glycols; D011142:Polyvinyl Alcohol; D011770:Pyruvate Kinase