title: ICAM-1 a ligand for LFA-1-dependent adhesion of B, T and myeloid cells.
abstract: Cell-cell adhesion is essential for many immunological functions. The LFA-1 molecule, a member of a superfamily of adhesion molecules, participates in adhesion which is critical to the function of each of the three major subsets of leukocytes: lymphocytes, monocytes and granulocytes. Putative LFA-1 ligands have been identified functionally in different laboratories using three different monoclonal antibodies that inhibit LFA-1-mediated leukocyte adhesion in particular model systems; however, there may be more than one LFA-1 ligand. We have directly compared the three relevant monoclonal antibodies, and show that each binds to the same molecule, intercellular-adhesion molecule-1 (ICAM-1). Most important, B, T and myeloid cells adhere specifically to purified ICAM-1-coated surfaces; such adhesion has distinctive requirements for Mg2+ and Ca2+. This constitutes biochemical evidence that ICAM-1 functions as a ligand for LFA-1-dependent adhesion by a variety of leukocytes.
authors.affiliation: Immunology Branch, NCI, Bethesda, Maryland 20892.
authors.forename: M W
authors.initials: MW
authors.lastname: Makgoba
authors.forename: M E
authors.initials: ME
authors.lastname: Sanders
authors.forename: G E
authors.initials: GE
authors.lastname: Ginther Luce
authors.forename: M L
authors.initials: ML
authors.lastname: Dustin
authors.forename: T A
authors.initials: TA
authors.lastname: Springer
authors.forename: E A
authors.initials: EA
authors.lastname: Clark
authors.forename: P
authors.initials: P
authors.lastname: Mannoni
authors.forename: S
authors.initials: S
authors.lastname: Shaw
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D000954:Antigens, Surface / Q000276:immunology / Q000502:physiology*; D001402:B-Lymphocytes / Q000502:physiology*; D002118:Calcium / Q000502:physiology; D002448:Cell Adhesion; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D016169:Lymphocyte Function-Associated Antigen-1; D008274:Magnesium / Q000502:physiology; D013602:T-Lymphocytes, Cytotoxic / Q000502:physiology*; D014407:Tumor Cells, Cultured
chemical_list: D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D016169:Lymphocyte Function-Associated Antigen-1; D008274:Magnesium; D002118:Calcium