Bactericidal activity of middle ear effusion on a single isolate of non-typable Haemophilus influenzae.
Bactericidal activity of middle ear effusion (MEE) from children with otitis media with effusion (OME) on one strain of non-typable Haemophilus influenzae was measured and was compared with that of normal human serum. H. influenzae culture negative MEEs had a bactericidal activity on one strain of non-typable H. influenzae (P less than 0.01), and the activity of serous effusions was significantly stronger than that of mucoid ones (P less than 0.01). The bactericidal activity of 20% pooled MEE was almost equal to that of 4% normal human serum. Heat treatment (56 degrees C, 30 min) abolished this activity of both pooled MEE and normal human serum. Addition of fresh guinea pig serum (GPS) as a complement source to the heat-treated pooled MEE restored this activity, while its addition to the heat-treated normal human serum failed to do so. These results suggest that complement factor is important in the bactericidal activity of MEE.
Shimizu
T
T
Department of Otorhinolaryngology, Mie University School of Medicine, Tsu Japan.
Harada
T
T
Majima
Y
Y
Sakakura
Y
Y
D000293:Adolescent; D000818:Animals; D001770:Blood Bactericidal Activity; D002648:Child; D002675:Child, Preschool; D003165:Complement System Proteins / Q000502:physiology; D004432:Ear, Middle / Q000276:immunology*; D005122:Exudates and Transudates / Q000276:immunology*; D006168:Guinea Pigs; D006193:Haemophilus influenzae / Q000254:growth & development*; D006801:Humans; D010034:Otitis Media with Effusion / Q000201:enzymology / Q000276:immunology*
D003165:Complement System Proteins