The protein factor which binds to the upstream activating sequence of Saccharomyces cerevisiae ENO1 gene.
Using a gel retardation assay it was shown that the 87 bp DNA fragment (UAS87) containing the upstream activating sequence (UAS) of S. cerevisiae EN01 gene and a nuclear extract gave rise to three migration-retarded species specific to UAS87. Heat- or proteinase-treatment of the nuclear extract revealed that these species were protein-DNA complexes. The precise binding region of the protein identified by DNaseI protection analysis was found to include a CCAAACA sequence which forms a dyad-symmetrical structure. The amount of one of the three migration-retarded species significantly increased when cells were grown in medium containing a gluconeogenic carbon source. The introduction of pGCR8, a multicopy plasmid containing GCR1 gene, a regulatory gene controlling the expression of several glycolytic enzymes, showed no effect on the amount of three migration-retarded species.
Machida
M
M
National Chemical Laboratory for Industry, Kagaku Gijutsu Kenkyusho, Tsukuba Research Center, Ibaraki, Japan.
Uemura
H
H
Jigami
Y
Y
Tanaka
H
H
D001483:Base Sequence; D005786:Gene Expression Regulation; D005796:Genes; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000378:metabolism*; D010751:Phosphopyruvate Hydratase / Q000235:genetics*; D010957:Plasmids; D011485:Protein Binding; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*
D009687:Nuclear Proteins; D010751:Phosphopyruvate Hydratase