Immunohistochemical localization of gamma-aminobutyric acid- and aspartate-containing neurons in the rat deep cerebellar nuclei.
The immunohistochemical localization of gamma-aminobutyric acid (GABA)- and aspartate-containing neurons was evidenced in the rat deep cerebellar nuclei, using purified antisera to GABA and aspartate, respectively. Most GABA-containing neurons had small cell bodies and were scattered unevenly throughout the deep cerebellar nuclei. The medial cerebellar nucleus had a few GABA-containing cell bodies and interpositus and lateral nuclei contained many GABA-containing cell bodies. GABA-containing terminals were distributed throughout the nuclei. A large number of various-sized aspartate-containing cells were present in the deep cerebellar nuclei and most of these cells were large or medium-size. Aspartate-containing cells in the medial, interpositus and lateral nucleus were surrounded by GABA-like immunoreactive terminals, thereby suggesting the modulation of aspartate-containing neurons by GABA-ergic fibers from Purkinje cells.
Kumoi
K
K
Department of Pharmacology, Kobe University School of Medicine, Japan.
Saito
N
N
Kuno
T
T
Tanaka
C
C
D000818:Animals; D001127:Arginine Vasopressin / Q000031:analogs & derivatives / Q000494:pharmacology; D001224:Aspartic Acid / Q000032:analysis* / Q000276:immunology; D002529:Cerebellar Nuclei / Q000166:cytology*; D005260:Female; D005455:Fluorescent Antibody Technique; D007106:Immune Sera; D008297:Male; D009474:Neurons / Q000166:cytology*; D009928:Organ Specificity; D051381:Rats; D005680:gamma-Aminobutyric Acid / Q000032:analysis* / Q000276:immunology
D007106:Immune Sera; D001127:Arginine Vasopressin; D001224:Aspartic Acid; D005680:gamma-Aminobutyric Acid; C025043:vasopressin, 1-(1-mercaptocyclohexaneacetic acid)-2-(O- methyl-L-tyrosine)-8-L-arginine-