[Enzymatic synthesis of L-malic acid from fumaric acid using immobilized Escherichia coli cells].
Optimal conditions were chosen for cultivation of Escherichia coli 85 cells with a rather high fumarate-hydratase activity on a cheap medium containing no edible raw material. An active biocatalyst for the synthesis of L-malic acid from fumaric acid was obtained based on E. coli 85 cells immobilized in carrageenan. The enzymatic synthesis of L-malic acid from potassium fumarate was kinetically studied and optimized. Some thermodynamic parameters of fumaric acid hydration into malic acid were determined. A technique for assaying the reaction mixture was developed that involved high performance liquid chromatography.
Verevkin
A N
AN
Zueva
N N
NN
Iakovleva
V I
VI
Sokolova
E N
EN
Golovkina
G P
GP
D004926:Escherichia coli / Q000201:enzymology / Q000378:metabolism*; D005649:Fumarate Hydratase / Q000378:metabolism*; D005650:Fumarates / Q000378:metabolism*; D007700:Kinetics; D008293:Malates / Q000096:biosynthesis*
D005650:Fumarates; D008293:Malates; C030298:malic acid; C032005:fumaric acid; D005649:Fumarate Hydratase
Enzimaticheskiĭ sintez L-iablochnoĭ kisloty iz fumarovoĭ s pomoshch'iu immobilizovannykh kletok Escherichia coli.