title: A competitive inhibition enzyme immunoassay for detection and quantification of organophosphorus compounds.
abstract: A sensitive and specific method for detection and quantification of methyl phosphonic acid, p-aminophenyl 1,2,2-trimethyl-propyl diester (MATP) as a model substance for organophosphorus compounds is described. Different procedures for coupling the haptenic group for immunization, purification and immobilization allowed the detection of hapten-specific antibodies. The competitive inhibition enzyme immunoassay (CIEIA), using purified chicken and rabbit IgG-antibodies, was able to detect MATP-concentrations as low as 10(-10) mol/l. Based upon our results, we postulate that the CIEIA represents a good alternative to the customary diagnosis of organophosphate intoxications, measuring blood cholinesterase activity.
authors.affiliation: Institut für Physiologie, Physiologische Chemie und Ernährungsphysiologie, Tierärztliche Fakultät, Universität München, Bundesrepublik Deutschland.
authors.forename: P
authors.initials: P
authors.lastname: Schmidt
authors.forename: R
authors.initials: R
authors.lastname: Kühlmann
authors.forename: U
authors.initials: U
authors.lastname: Lösch
mesh_terms: D000818:Animals; D000906:Antibodies / Q000302:isolation & purification; D002645:Chickens / Q000276:immunology; D007124:Immunoenzyme Techniques; D007202:Indicators and Reagents; D009943:Organophosphorus Compounds / Q000032:analysis*; D011817:Rabbits / Q000276:immunology
chemical_list: D000906:Antibodies; D007202:Indicators and Reagents; D009943:Organophosphorus Compounds; C056499:4-aminophenylphosphonic acid methyl-1,2,2-trimethylpropyl diester