title: Postnatal B cell development: influence of trinitrobenzenesulfonic acid treatment during pregnancy.
abstract: Prenatal treatment with a reactive hapten may be well suited for analyzing the establishment of self tolerance because the hapten binds ubiquitously to proteins and cells and persists for a long period in the developing organism. Based on this consideration, pregnant BALB/c mice were treated with 2,4,6-trinitrobenzenesulfonic acid (TNBS), searching for differences in 2,4,6-trinitrophenyl (TNP) responsiveness in their offspring as compared to litters of untreated mice. The frequency of TNP-specific T-independent B cells of litters from TNBS-treated mothers was very low at birth and remained below 10% of controls until the age of 42 days. On the contrary, in 8-day-old prenatally TNBS-treated litters, the frequency of TNP-specific T-dependent B cells was higher than in controls. Expansion of TNP-specific B cells after antigenic stimulation of control mice started at the age of 3-4 weeks and expansion rates increased with age, while in prenatally TNBS-treated mice, significant expansion rates were seen at the age of 2 weeks only. Yet, after restimulation with TNP-lipopolysaccharide or with a TNP-anti-TNP conjugate, but not after restimulation with TNP-ovalbumin, similar numbers of plaque-forming cells (PFC) were observed with spleen cells of prenatally untreated and TNBS-treated mice, the latter revealing an exceptional predominance of IgG PFC. Thus, TNP-specific B cells were not deleted, but prenatal TNBS treatment resulted in an altered composition of TNP-specific B cell subpopulations, their regulation differing qualitatively from the one observed in prenatally untreated mice.
authors.affiliation: Institute for Radiology and Pathophysiology, German Cancer Research Center, Heidelberg, FRG.
authors.forename: M
authors.initials: M
authors.lastname: Zöller
mesh_terms: D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn / Q000276:immunology; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology*; D002454:Cell Differentiation; D005260:Female; D007108:Immune Tolerance; D008211:Lymphocyte Cooperation; D008431:Maternal-Fetal Exchange; D051379:Mice; D009578:Nitrobenzenes / Q000276:immunology*; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000276:immunology*; D013154:Spleen / Q000276:immunology; D014302:Trinitrobenzenesulfonic Acid / Q000276:immunology*
chemical_list: D009578:Nitrobenzenes; D014302:Trinitrobenzenesulfonic Acid