N-myc proto-oncogene expression can induce DNA replication in Balb/c 3T3 fibroblasts.
N-myc and c-myc are structurally and functionally related proto-oncogenes. C-myc expression plays an essential role towards the induction of DNA replication following mitogen stimulation of quiescent cells. We have examined whether N-myc expression can stimulate cellular DNA synthesis. When growth factor-deprived quiescent Balb/c 3T3 fibroblasts harboring a glucocorticoid-inducible N-myc gene were stimulated with dexamethasone, the resultant N-myc expression induced S-phase DNA synthesis. Hence, functional homology between N-myc and c-myc extends to their joint ability to stimulate DNA replication.
Cavalieri
F
F
Department of Biochemistry and Molecular Biophysics, Columbia University College of Physicians and Surgeons, New York, New York.
Goldfarb
M
M
D000818:Animals; D002460:Cell Line; D004261:DNA Replication; D003907:Dexamethasone / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects; D006657:Histones / Q000235:genetics; D051379:Mice; D011518:Proto-Oncogene Proteins / Q000502:physiology*; D011519:Proto-Oncogenes; D012333:RNA, Messenger / Q000235:genetics
D006657:Histones; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D003907:Dexamethasone