Anti-Candida albicans activity of Pichia anomala as determined by a growth rate reduction assay.
Killer toxin activity of Pichia anomala WC65 appeared fungicidal for P. bimundalis WC38 and fungistatic for Candida albicans RC1. Inhibitory activity against sensitive C. albicans showed a linear relationship between toxin concentrations and the inverse of the reduced growth rates. The plot of toxin concentrations against growth rates was hyperbolic, as is characteristic of saturation kinetics. Sensitivity of C. albicans to the toxin decreased with increased cell age. The measurement of growth rate reduction provided a simple and accurate method for quantitation of toxin.
Sawant
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Laboratory for Microbial and Biochemical Sciences, Georgia State University, Atlanta 30303.
Abdelal
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Ahearn
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D000935:Antifungal Agents / Q000096:biosynthesis / Q000633:toxicity*; D002176:Candida albicans / Q000187:drug effects / Q000254:growth & development*; D003470:Culture Media / Q000494:pharmacology; D005656:Fungal Proteins / Q000096:biosynthesis / Q000633:toxicity*; D055646:Killer Factors, Yeast; D009183:Mycotoxins / Q000096:biosynthesis / Q000633:toxicity*; D010843:Pichia / Q000378:metabolism / Q000502:physiology*; D004718:Saccharomycetales / Q000502:physiology*; D013997:Time Factors
D000935:Antifungal Agents; D003470:Culture Media; D005656:Fungal Proteins; D055646:Killer Factors, Yeast; D009183:Mycotoxins