Induction of neuronal differentiation in PC12 cells by B-cell stimulatory factor 2/interleukin 6.
B-cell stimulatory factor 2 (BSF-2) is a lymphokine which induces the final maturation of B cells. BSF-2 acts on a variety of cells other than B cells, and moreover, expression of BSF-2 mRNA is detected in interleukin-1 beta-stimulated glioblastoma and astrocytoma cell lines. Here, we studied the function of BSF-2 on pheochromocytoma PC12 cells, a model system for induction of neuronal differentiation. PC12 cells possess specific receptors for BSF-2. The BSF-2-stimulated PC12 cells expressed the c-fos proto-oncogene transiently, and they began to change morphologically to neurite-extending cells after several days. The number of voltage-dependent Na+ channels was also increased.
Satoh
T
T
Institute of Medical Science, University of Tokyo, Japan.
Nakamura
S
S
Taga
T
T
Matsuda
T
T
Hirano
T
T
Kishimoto
T
T
Kaziro
Y
Y
D000310:Adrenal Gland Neoplasms; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D015850:Interleukin-6; D007378:Interleukins / Q000494:pharmacology*; D007700:Kinetics; D009474:Neurons / Q000166:cytology*; D009857:Oncogenes / Q000187:drug effects; D010673:Pheochromocytoma; D012333:RNA, Messenger / Q000235:genetics; D014158:Transcription, Genetic / Q000187:drug effects
D015850:Interleukin-6; D007378:Interleukins; D012333:RNA, Messenger