Localization of calcitonin gene-related peptide (CGRP) at a neuronal nicotinic synapse.
The localization of calcitonin gene-related peptide (CGRP) in the parasympathetic cardiac ganglion of the frog was investigated with an immunofluorescence method. By doubly staining the whole ganglion with antibodies against a synaptic-vesicle antigen and anti-CGRP antibodies, we found that all of the boutons at the nicotinic synapses on the postganglionic neurons contained immunoreactivity against this peptide. In contrast, neither the muscarinic nor the adrenergic terminals of the postganglionic fibers on the cardiac muscle contained CGRP immunoreactivity. This suggests that CGRP is involved in the functions of the neuronal nicotinic synapses.
Peng
H B
HB
Department of Cell Biology and Anatomy, University of North Carolina, Chapel Hill 27599.
Chen
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D000818:Animals; D000911:Antibodies, Monoclonal; D001338:Autonomic Fibers, Postganglionic / Q000378:metabolism*; D015740:Calcitonin Gene-Related Peptide; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009479:Neuropeptides / Q000378:metabolism*; D011894:Rana pipiens; D011978:Receptors, Nicotinic / Q000378:metabolism*; D013569:Synapses / Q000276:immunology / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D009419:Nerve Tissue Proteins; D009479:Neuropeptides; D011978:Receptors, Nicotinic; D015740:Calcitonin Gene-Related Peptide