Molecular cloning and sequencing of coho salmon growth hormone cDNA.
A cDNA library was constructed using mRNA isolated from coho salmon pituitaries. By employing rainbow trout growth hormone cDNA as a probe, the coho salmon cDNA was isolated and the complete nucleotide (nt) sequence determined. The coding region contains 630 nt while the 5'- and 3'-untranslated regions are 64 and 489 nt in length, respectively. Comparison of the noncoding regions of coho and chum salmon cDNAs reveal identity at the 5' end but significant variation in the 3' end. Chum salmon and rainbow trout have identical amino acid (aa) sequences, but coho salmon growth hormone has a sequence that differs by 6 of the 188 predicted aa. Since salmonids are tetraploid, this difference may be the result of either divergence of the same growth hormone locus or of variation between different loci. Comparisons of the cDNA restriction maps of these three fish species suggest the former possibility.
González-Villaseñor
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LI
Department of Biology, Johns Hopkins University, Baltimore, MD 21218.
Zhang
P J
PJ
Chen
T T
TT
Powers
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DA
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D013006:Growth Hormone / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids; D012474:Salmon / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
D013006:Growth Hormone; D004247:DNA