Analysis of epidermal growth factor and epidermal growth factor receptor expression in human renal carcinoma cell cultures.
In cells derived from two human renal carcinomas only the precursor form of epidermal growth factor (EGF) was found. The binding assay revealed a high level of EGF receptor expression in both cell types tested. However, these receptors are not involved in the growth activity of the cells under in vitro conditions used. The source of DNA synthesis-stimulating activity found in conditioned media of the cells tested is discussed with respect to possible participation of TGF beta.
Sovová
V
V
Institute of Molecular Genetics, Czechoslovak Academy of Sciences, Praha.
Vydra
J
J
Cerná
H
H
Sloncová
E
E
Damková
M
M
Jakoubková
J
J
Hlozánek
I
I
D002292:Carcinoma, Renal Cell / Q000378:metabolism*; D002455:Cell Division; D002460:Cell Line; D004815:Epidermal Growth Factor / Q000096:biosynthesis* / Q000494:pharmacology; D066246:ErbB Receptors / Q000096:biosynthesis* / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D007680:Kidney Neoplasms / Q000378:metabolism*; D007700:Kinetics; D013498:Suramin / Q000494:pharmacology
D013498:Suramin; D004815:Epidermal Growth Factor; D066246:ErbB Receptors