The cloning and over-expression of PABA synthase in E. coli.
Both the genes encoding E. coli p-aminobenzoic acid synthase have been cloned and an overproducing strain has been obtained. The partial purification of the large subunit is described. The kinetic properties of the cloned enzyme, while similar to those reported for the B. subtilis enzyme, show some differences to those reported for the S. griseus enzyme.
McLeish
M J
MJ
Department of Pharmaceutical Chemistry, Victorian College of Pharmacy Ltd., Parkville, Vic., Australia.
Wookey
P J
PJ
Mortimer
K G
KG
D003001:Cloning, Molecular; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D005786:Gene Expression Regulation; D005798:Genes, Bacterial; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D000637:Transaminases / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism
C022309:PABA synthase; D000637:Transaminases