The potential use of microcalorimetry in predictive tests of the action of antineoplastic drugs on mammalian cells.
The effect of methotrexate (MTX) on cultured T-lymphoma cells in a growing suspension was measured by microcalorimetry. The effect of the drug could be observed within 2 h after its injection into the calorimetric vessel. A dose-response curve was constructed from the calorimetric results obtained with final concentrations of MTX in the range of 0.02-2.00 microM. In another series of experiments calorimetric values were determined after 8 h of MTX-cell interaction. These data were correlated with values for cell counts during an 18 h period following the removal of the drug. A clear correlation was found between the calorimetric and cytotoxicity data. Calorimetric results obtained within 8 h of MTX-cell interaction appear to be of value in predicting the antineoplastic effect of the drug.
Schön
A
A
Division of Thermochemistry, University of Lund, Sweden.
Wadsö
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I
D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002151:Calorimetry / Q000706:statistics & numerical data*; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D008223:Lymphoma / Q000378:metabolism*; D008727:Methotrexate / Q000494:pharmacology; D008832:Microchemistry; D013601:T-Lymphocytes / Q000187:drug effects; D014407:Tumor Cells, Cultured / Q000187:drug effects*
D000903:Antibiotics, Antineoplastic; D000970:Antineoplastic Agents; D008727:Methotrexate