[Evaluation of the vitamin D activity of yeasts with altered sterol metabolism].
The sterol content of Saccharomyces strains with altered ergosterol metabolism was studied by UV-spectrophotometry, thin-layer chromatography and chromatographic mass-spectroscopy. A technique for estimation of D-vitamin activity of the yeast strains is proposed. The irradiated biomass of the strains accumulated ergosta-5,7-dien-3 beta-ol and also cholesta-5,7,24-trien-3 beta-ol and cholesta-5,7,22,24-tetraen-3 beta-ol is characterized by high antirachitic activity.
Mikhaĭlova
N P
NP
Valinietse
M Iu
MIu
Bauman
V K
VK
V'iunov
K A
KA
D000818:Animals; D002645:Chickens; D004875:Ergosterol / Q000096:biosynthesis*; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000378:metabolism; D064030:S100 Calcium Binding Protein G / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet; D014807:Vitamin D / Q000096:biosynthesis*
D064030:S100 Calcium Binding Protein G; D014807:Vitamin D; D004875:Ergosterol
Otsenka D-vitaminnoĭ aktivnosti shtammov drozhzheĭ s izmenennym metabolizmom sterinov.