title: Alpha-sarcin cleaves ribosomal RNA at the alpha-sarcin site in the absence of ribosomal proteins.
abstract: alpha-Sarcin is capable of specifically cleaving the single phosphodiester bond in the "alpha-sarcin site" of both Escherichia coli and Saccharomyces cerevisiae large rRNAs in the absence of ribosomal proteins. With both sources of rRNA, the rate of cleavage was comparable with and without ribosomal proteins but more complete cleavage was observed in the absence of ribosomal proteins. These observations contrast with earlier findings and indicate that ribosomal proteins are not essential to the unique specificity of the cleavage of rRNA by alpha-sarcin.
authors.affiliation: Department of Biochemistry, University of Minnesota, Minneapolis 55455.
authors.forename: S P
authors.initials: SP
authors.lastname: Miller
authors.forename: J W
authors.initials: JW
authors.lastname: Bodley
mesh_terms: D004722:Endoribonucleases; D004926:Escherichia coli / Q000378:metabolism; D005656:Fungal Proteins / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D011500:Protein Synthesis Inhibitors / Q000378:metabolism*; D012335:RNA, Ribosomal / Q000187:drug effects / Q000378:metabolism*; D012269:Ribosomal Proteins / Q000502:physiology; D012270:Ribosomes / Q000187:drug effects / Q000378:metabolism
chemical_list: D005656:Fungal Proteins; D011500:Protein Synthesis Inhibitors; D012335:RNA, Ribosomal; D012269:Ribosomal Proteins; C014801:alpha-sarcin; D004722:Endoribonucleases