[Kinetic analysis of the action of cetyltrimethylammonium bromide on the membranes of the capsular variant of Escherichia coli CA189 recorded by ethidium bromide fluorescence].
A mathematical model, describing the kinetics of ethidium bromide probe fluorescence in the suspension of E. coli CA 189 capsular antigen after introduction of cetyltrimethylammonium bromide (a detergent), has been developed. Experimental testing of this mathematical model has confirmed that it describes the kinetics of the probe fluorescence augmentation sufficiently accurately. The new model permits the quantitative characterization of the rate of surfactant diffusion through the bacterial capsule.
Puchkov
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EO
Aponin
Iu M
IuM
Irkhin
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Vaniakin
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EN
D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D002463:Cell Membrane Permeability / Q000187:drug effects; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000494:pharmacology*; D004058:Diffusion; D004926:Escherichia coli / Q000187:drug effects* / Q000378:metabolism; D004996:Ethidium / Q000493:pharmacokinetics; D005456:Fluorescent Dyes; D014644:Genetic Variation / Q000187:drug effects*; D008954:Models, Biological; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology*; D013050:Spectrometry, Fluorescence
D002593:Cetrimonium Compounds; D005456:Fluorescent Dyes; D000644:Quaternary Ammonium Compounds; D004996:Ethidium; D000077286:Cetrimonium
Analiz kinetiki deĭstviia tsetiltrimetilammoniĭbromida na membrany kapsul'nogo varianta Escherichia coli CA189, registriruemoĭ po fliuorestsentsii bromistogo étidiia.