Long term cryopreservation of the amastigote stages of hemoflagellates.
Tissue homogenates containing amastigotes of either Leishmania donovani, L. tropica, or Trypanosoma cruzi were rapidly frozen with 10% glycerol as cryoprotectant. Viability and pathogenicity were maintained for at least 23 years with the Khartoum strain of L. donovani, 22 years with the Malakal strain of L. donovani, and 7 years for L. tropica and T. cruzi. Similar results over a shorter period of time were obtained with a slow-freezing technique.
Yaeger
R G
RG
Department of Tropical Medicine, Tulane University School of Public Health and Tropical Medicine, New Orleans, Louisiana 70112.
D000818:Animals; D006224:Cricetinae; D005615:Freezing; D006801:Humans; D007893:Leishmania donovani / Q000254:growth & development*; D007895:Leishmania tropica / Q000254:growth & development*; D011309:Preservation, Biological; D014349:Trypanosoma cruzi / Q000254:growth & development*