A theoretical investigation of the sequence specificity in the binding of the antitumor drug anthramycin to DNA.
A theoretical study is presented concerning DNA-anthramycin adducts. By explicit energy minimisations using a semi-empirical energy formula and an advanced algorithm the structural properties and the energetics of this system are analysed. The results obtained demonstrate that the formation of a covalently bound adduct in which anthramycin is attached to the N2 site of a guanine within a DNA fragment is accompanied by a considerable change in the nucleic acid conformation as confirmed by recent experimental evidence. With the use of the "SIR" methodology for treating DNA flexibility the general features of this change are characterised. The sequence specificity of anthramycin binding is investigated and the important role of sequence dependent nucleic acid flexibility brought to light. This theoretical treatment thus provides new elements for the interpretation of the origins of ligand binding specificities.
Zakrzewska
K
K
Institute de Biologie Physico-Chimique, Laboratoire de Biochimie Thórique associé au C.N.R.S. Paris, France.
Pullman
B
B
D000876:Anthramycin; D001483:Base Sequence; D001570:Benzodiazepinones; D001665:Binding Sites; D004247:DNA; D008958:Models, Molecular; D009690:Nucleic Acid Conformation
D001570:Benzodiazepinones; D000876:Anthramycin; D004247:DNA