Esterification of retinol in rat liver. Possible participation by cellular retinol-binding protein and cellular retinol-binding protein II.
Retinol bound to cellular retinol-binding protein (CRBP) was available for esterification by liver microsomes in the absence of exogenous acyl donors. Moreover, exogenous acyl-CoA gave little or no stimulation of ester production over what was observed with the endogenous acyl donor. In contrast, unbound retinol was esterified in an acyl-CoA-dependent reaction. The presence of two different enzyme activities, acyl-CoA-dependent and -independent, was demonstrated by differential sensitivities to several enzyme inhibitors. The enzyme reaction with retinol-CRBP and endogenous acyl donor produced retinyl esters normally found in vivo in liver. In addition, rates of esterification with this system were sufficient to maintain liver stores. Liver also contains cellular retinol-binding protein, type II (CRBP(II] during the perinatal period. Radioimmunoassay revealed highest levels of CRBP(II) in liver 3-4 days after birth. Examination of retinol esterification by microsomes from the liver of 3-day-old rats revealed a retinyl ester synthase activity with lower Km and higher Vmax than that found in the adult. The activity could use either retinol-CRBP or retinol-CRBP(II) and an endogenous acyl donor. The microsomes from 3-day-old liver had greater esterifying ability than microsomes from adult liver, perhaps due to the presence of two retinyl ester synthase enzymes.
Ong
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Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37232.
MacDonald
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Gubitosi
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D000214:Acyl Coenzyme A / Q000494:pharmacology; D000217:Acyltransferases / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism; D002729:Chloromercuribenzoates / Q000494:pharmacology; D004951:Esterification; D005033:Ethylmaleimide / Q000494:pharmacology; D007124:Immunoenzyme Techniques; D008099:Liver / Q000254:growth & development; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism*; D010171:Palmitoyl Coenzyme A / Q000494:pharmacology; D010664:Phenylmethylsulfonyl Fluoride / Q000494:pharmacology; D011485:Protein Binding; D051381:Rats; D051142:Retinol O-Fatty-Acyltransferase; D012177:Retinol-Binding Proteins / Q000378:metabolism*; D054840:Retinol-Binding Proteins, Cellular; D014801:Vitamin A / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000378:metabolism; D020245:p-Chloromercuribenzoic Acid
D000214:Acyl Coenzyme A; D002729:Chloromercuribenzoates; C518461:Rbp2 protein, rat; D012177:Retinol-Binding Proteins; D054840:Retinol-Binding Proteins, Cellular; D014801:Vitamin A; D010171:Palmitoyl Coenzyme A; D010664:Phenylmethylsulfonyl Fluoride; D020245:p-Chloromercuribenzoic Acid; D000217:Acyltransferases; D051142:Retinol O-Fatty-Acyltransferase; D005033:Ethylmaleimide