title: Stimulation of an alpha like DNA polymerase by v-myc related protein of Halobacterium halobium.
abstract: Partial DNA sequencing of a genomic clone of the archaebacterium Halobacterium halobium, which hybridized with an avian v-myc probe, showed especially the presence, in the organism of one of the conserved regions through myb, myc and adenovirus E1a oncogenes. The archaebacterial deduced amino acid sequence displayed significant homology with the v-myc gene product. In accordance with the partial DNA sequencing which assured a sufficient homology to have similar epitopes, a protein having a molecular weight of 70,000 and possessing high antigenicity with a polyclonal antiserum against avian v-myc protein was isolated and purified from H. halobium extracts. The purified v-myc like protein stimulated in vitro DNA synthesis carried out by the alpha like DNA polymerase of H. halobium.
authors.affiliation: Institut Jacques Monod, Université Paris VII, France.
authors.forename: K
authors.initials: K
authors.lastname: Ben-Mahrez
authors.forename: W
authors.initials: W
authors.lastname: Sougakoff
authors.forename: M
authors.initials: M
authors.lastname: Nakayama
authors.forename: M
authors.initials: M
authors.lastname: Kohiyama
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004257:DNA Polymerase II / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D006217:Halobacterium / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D016274:Oncogene Protein p55(v-myc); D009857:Oncogenes; D012191:Retroviridae Proteins / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
chemical_list: D004269:DNA, Bacterial; D016274:Oncogene Protein p55(v-myc); D012191:Retroviridae Proteins; D004257:DNA Polymerase II