The biology of the colony stimulating factors.
Previous in vitro studies with recombinant human Interleukin 3 (IL-3) in serum-replete culture conditions suggested that IL-3 induced the complete differentiation of multipotent and both, erythroid and myeloid progenitor cells. In contrast, serum-free cultures have shown that alone, IL-3, and to a lesser extent granulocyte-macrophage colony-stimulating factor (GM-CSF), are inadequate differentiation stimuli. However, in combination with the lineage-specific growth factors, erythropoietin, granulocyte colony stimulating factor (G-CSF), and macrophage CSF (M-CSF), complete recovery of erythroid, granulocyte, and macrophage colonies, respectively, was obtained. GM-CSF is produced by a variety of mesenchymal cells as well as monocytes and T lymphocytes; in contrast, IL-3 production appears to be restricted to lectin stimulated T lymphocytes. These studies indicate that the CSFs may be more effective in vivo when used as combinations rather than as single factors. The restricted production of IL-3 by activated T cells suggests that IL-3 may be released during infections and may play an important role during stress.
Sieff
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Division of Hematology and Pediatric Oncology, Dana-Farber Cancer Institute, Boston, Massachusetts.
Niemeyer
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CM
D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D003115:Colony-Stimulating Factors / Q000235:genetics / Q000494:pharmacology / Q000502:physiology*; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000187:drug effects; D006801:Humans; D007377:Interleukin-3 / Q000235:genetics / Q000494:pharmacology / Q000502:physiology; D011994:Recombinant Proteins / Q000494:pharmacology
D003115:Colony-Stimulating Factors; D007377:Interleukin-3; D011994:Recombinant Proteins