title: FLP recombinase is an enzyme.
abstract: The FLP protein of the yeast 2-microns plasmid catalyzes intermolecular site-specific recombination with a turnover number of approximately equal to 0.12 min-1 (per FLP monomer) for relaxed DNA substrates. Under conditions that enhance its stability, the protein can be used in catalytic rather than stoichiometric amounts. The reaction rate exhibits a strong dependence on FLP protein concentration even when the protein is present in excess relative to available recombination sites.
authors.affiliation: Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin-Madison 53706.
authors.forename: C A
authors.initials: CA
authors.lastname: Gates
authors.forename: M M
authors.initials: MM
authors.lastname: Cox
mesh_terms: D001483:Base Sequence; D004254:DNA Nucleotidyltransferases / Q000378:metabolism*; D004271:DNA, Fungal / Q000378:metabolism; D005656:Fungal Proteins / Q000378:metabolism*; D010957:Plasmids; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000201:enzymology*
chemical_list: D004271:DNA, Fungal; D005656:Fungal Proteins; D004254:DNA Nucleotidyltransferases; C045119:FLP recombinase