Biological characterization of charge isomers of human growth hormone.
Since deamidation of the human GH molecule may alter the manner and extent to which the hormone is cleaved by proteases, and since it has been repeatedly suggested that proteolytic processing is required for the expression of certain of the activities of GH, the present study was conducted to determine whether the biological activity profiles of more acidic forms of human GH are altered. Three charge isomers, GH-b, GH-c and GH-d, representing primarily deamidated forms, were isolated from a native human GH preparation (Crescormon) in amounts adequate for characterization of their biological activities. All three were essentially equipotent in a radioimmunoassay for human GH. When assessed for growth-promoting activity in the hypophysectomized rat, the isomers were again equipotent with each other and with the GH preparation from which they were derived. The charge isomers also had significant in vitro insulin-like activity on isolated rat adipose tissue and diabetogenic activity in the ob/ob mouse. Thus, the biological activity profiles of these charge isomers of human GH do not differ greatly from one another.
Skottner
A
A
KabiVitrum Peptide Hormones, Research and Development, KabiVitrum AB, Stockholm, Sweden.
Forsman
A
A
Skoog
B
B
Kostyo
J L
JL
Cameron
C M
CM
Adamafio
N A
NA
Thorngren
K G
KG
Hagerman
M
M
D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D005951:Glucose Tolerance Test; D006128:Growth / Q000187:drug effects; D013006:Growth Hormone / Q000032:analysis / Q000494:pharmacology*; D006801:Humans; D007328:Insulin / Q000378:metabolism; D007525:Isoelectric Focusing; D007536:Isomerism; D051379:Mice; D008815:Mice, Inbred Strains; D008970:Molecular Weight; D051381:Rats; D011919:Rats, Inbred Strains
D007328:Insulin; D013006:Growth Hormone