A reliable method for the analysis of human MHC class II alpha and beta chains by Western blotting of IEF gels.
The solubility and ensuing transfer problems associated with IEF separation and blotting of membrane proteins have been eliminated by confining NP40 to the sample region of the gel and replacing it with octylglycoside in the separating gel. This permitted excellent separation of both the alpha and beta chains of the human MHC antigens and their subsequent detection by Western blotting.
Cohen
B B
BB
MRC Clinical and Population Cytogenetics Unit, Western General Hospital, Edinburgh, U.K.
D003902:Detergents; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D006681:HLA-D Antigens / Q000032:analysis*; D006801:Humans; D007163:Immunosorbent Techniques; D007525:Isoelectric Focusing / Q000379:methods*; D046911:Macromolecular Substances; D013501:Surface-Active Agents
D003902:Detergents; D006681:HLA-D Antigens; D046911:Macromolecular Substances; D013501:Surface-Active Agents