title: Simultaneous determination of mefloquine and chloroquine in biological fluids using high-performance liquid chromatography.
abstract: A high-performance liquid chromatography (HPLC) method for the simultaneous determination of mefloquine and chloroquine in biological fluids is described. Methaqualone is used as an internal standard. After a single-step extraction, achieved from alkalinized samples with diethyl ether, a separation is obtained using a mu-Bondapak phenyl column with a mobile phase of acetonitrile and sodium acetate buffer. Detection is done at 305 nm. The lower detection limits are 3 and 10 micrograms/l for mefloquine and chloroquine, respectively. Thus, this method appears to be simple, rapid, very sensitive and applicable in pharmaco-clinical, toxicological and forensic analyses.
authors.affiliation: Institut de Médecine Légale, Strasbourg.
authors.forename: J M
authors.initials: JM
authors.lastname: Lamant
authors.forename: P
authors.initials: P
authors.lastname: Kintz
authors.forename: A
authors.initials: A
authors.lastname: Tracqui
authors.forename: P
authors.initials: P
authors.lastname: Mangin
authors.forename: A A
authors.initials: AA
authors.lastname: Lugnier
authors.forename: A J
authors.initials: AJ
authors.lastname: Chaumont
mesh_terms: D000962:Antimalarials / Q000097:blood*; D002738:Chloroquine / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D015767:Mefloquine; D008702:Methaqualone / Q000097:blood; D011804:Quinolines / Q000097:blood*
chemical_list: D000962:Antimalarials; D011804:Quinolines; D008702:Methaqualone; D002738:Chloroquine; D015767:Mefloquine