Enzyme immunoassay of cortisol in human plasma using penicillinase as label.
An enzyme immunoassay for cortisol in human plasma using an antiserum raised against cortisol-3-O-carboxy-methyloxime bovine serum albumin and cortisol-21-hemisuccinate conjugated to penicillinase as tracer is described. Although employing immunoassay plates for separation of antigen-antibody complex from the free components was less time consuming, the slope and sensitivity of the standard curve were improved by the addition of goat anti-rabbit gamma globulin for precipitating the complex. There was good correlation between radioimmunoassay and enzyme immunoassay results obtained for cortisol levels present in normal human plasma.
Shrivastav
T G
TG
Department of Reproductive Biomedicine, National Institute of Health and Family Welfare, New Delhi, India.
Kumari
G L
GL
Rao
P N
PN
D005260:Female; D006801:Humans; D006854:Hydrocortisone / Q000097:blood*; D007124:Immunoenzyme Techniques; D008297:Male; D010405:Penicillinase; D012016:Reference Values
D010405:Penicillinase; D006854:Hydrocortisone