[Clonogenic cultivation of bone marrow fibroblast precursors in human myeloproliferative diseases].
Techniques of clonogenic cultivation with the application of xenogenous feeder (rabbit irradiated bone marrow) were used to study a number of bone marrow colony-forming cells (CFU-F) in 70 patients. A significant increase of CFU-F is observed in chronic myelocytic leukemia and in hepatosplenomegalies of non-leukemic origin CFU-F decreases considerably in the cases of myelofibrosis. Trypsinisation of the bone marrow taken from the cases of myelofibrosis results in a sharp CFU-F increase.
Kolosova
L Iu
LIu
Vladimirskaia
E B
EB
Kovaleva
L G
LG
D001853:Bone Marrow / Q000473:pathology*; D002999:Clone Cells / Q000473:pathology; D003114:Colony-Forming Units Assay; D003584:Cytological Techniques; D005347:Fibroblasts / Q000473:pathology; D006801:Humans; D009196:Myeloproliferative Disorders / Q000473:pathology*; D013234:Stem Cells / Q000473:pathology*
Klonogennoe kul'tivirovanie predshestvennikov kostno-mozgovykh fibroblastov pri mieloproliferativnykh zabolevaniiakh cheloveka.