Osmotic control of glycine betaine biosynthesis and degradation in Rhizobium meliloti.
Intracellular accumulation of glycine betaine has been shown to confer an enhanced level of osmotic stress tolerance in Rhizobium meliloti. In this study, we used a physiological approach to investigate the mechanism by which glycine betaine is accumulated in osmotically stressed R. meliloti. Results from growth experiments, 14C labeling of intermediates, and enzyme activity assays are presented. The results provide evidence for the pathway of biosynthesis and degradation of glycine betaine and the osmotic effects on this pathway. High osmolarity in the medium decreased the activities of the enzymes involved in the degradation of glycine betaine but not those of enzymes that lead to its biosynthesis from choline. Thus, the concentration of the osmoprotectant glycine betaine is increased in stressed cells. This report demonstrates the ability of the osmolarity of the growth medium to regulate the use of glycine betaine as a carbon and nitrogen source or as an osmoprotectant. The mechanisms of osmoregulation in R. meliloti and Escherichia coli are compared.
Smith
L T
LT
Plant Growth Laboratory, University of California, Davis 95616.
Pocard
J A
JA
Bernard
T
T
Le Rudulier
D
D
D000429:Alcohol Oxidoreductases / Q000378:metabolism; D001622:Betaine / Q000096:biosynthesis / Q000378:metabolism*; D055598:Chemical Phenomena; D002621:Chemistry; D002794:Choline / Q000378:metabolism; D003470:Culture Media; D012695:L-Serine Dehydratase / Q000378:metabolism; D009994:Osmolar Concentration; D009997:Osmotic Pressure; D012231:Rhizobium / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D014882:Water-Electrolyte Balance
D003470:Culture Media; D001622:Betaine; D000429:Alcohol Oxidoreductases; C019888:choline oxidase; D012695:L-Serine Dehydratase; D002794:Choline