title: Comparative effects of insulin and refeeding on DNA synthesis, HMP shunt and cholesterogenesis in diabetic and fasted rats.
abstract: DNA synthesis, cholesterogenesis and the enzymes of the hexosemonophosphate (HMP) shunt pathway were investigated in liver of diabetic rats treated with insulin and in fasted/re-fed rats. Both insulin and refeeding were found to induce liver cell proliferation, accompanied by a remarkable increase in cholesterogenesis. An enhancement of glucose-6-phosphate dehydrogenase (G6PD) and 6-phosphogluconate dehydrogenase (G6PD) activities was also found in insulin-treated diabetic rats and in re-fed rats, supporting the concept that these two enzymes are involved in the proliferative process. Since insulin did not exert the same biochemical effects in a non replicating cell population, such as in insulin-treated normal rats, these studies provide new evidence of a close correlation between DNA, cholesterol synthesis and HMP shunt enzymes during cell proliferation.
authors.affiliation: Istituto di Farmacologia e Patologia Biochimica, University of Cagliari, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Dessi
authors.forename: C
authors.initials: C
authors.lastname: Chiodino
authors.forename: B
authors.initials: B
authors.lastname: Batetta
authors.forename: M
authors.initials: M
authors.lastname: Armeni
authors.forename: M F
authors.initials: MF
authors.lastname: Mulas
authors.forename: P
authors.initials: P
authors.lastname: Pani
mesh_terms: D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002784:Cholesterol / Q000096:biosynthesis*; D004247:DNA / Q000096:biosynthesis*; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism* / Q000473:pathology; D005215:Fasting; D007328:Insulin / Q000494:pharmacology*; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008297:Male; D010427:Pentose Phosphate Pathway / Q000187:drug effects*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D007328:Insulin; D004247:DNA; D002784:Cholesterol