Rapid and transient induction of c-fos, c-myc and c-Ha-ras in rat liver following glycine administration.
Administration of glycine (2.5 mmoles/100 g., i.p.) results in an increased expression of several cell cycle dependent genes such as c-fos, c-myc and c-Ha-ras in the rat liver. The increased expression could be noticed as early as 20-40 minutes and declined by 2 hours following glycine administration. The rapid rise and decline in the mRNA levels of c-fos, c-myc and c-Ha-ras in response to glycine is of significance because in response to a wide variety of growth stimuli, these proto-oncogenes exhibit a temporal sequence in their expression; for example, the expression of c-fos precedes that of c-myc, which in turn precedes the increased expression of c-Ha-ras. The experimental model using a simple amino acid such as glycine will be useful in exploring some of the mechanisms of regulation of expression of these proto-oncogenes.
Vasudevan
S
S
Department of Pathology, University of Toronto, Ontario, Canada.
Lee
G
G
Rao
P M
PM
Rajalakshmi
S
S
Sarma
D S
DS
D000818:Animals; D002453:Cell Cycle; D011905:Genes, ras / Q000187:drug effects*; D005998:Glycine / Q000494:pharmacology*; D008099:Liver / Q000166:cytology / Q000187:drug effects*; D008297:Male; D011519:Proto-Oncogenes / Q000187:drug effects*; D051381:Rats; D011916:Rats, Inbred F344; D014158:Transcription, Genetic / Q000187:drug effects*
D005998:Glycine