A contribution to the study of damage and regeneration of hemopoiesis during fractionated irradiation and repeated bone marrow transplantation.
The experiment was aimed at studying two contradictory actions: damage to hemopoietic organs during application of radiation doses per fraction and regenerative efforts of the organism supported by repeated bone marrow transplantation. The mice received doses of 3 Gy of 60Co-gamma rays total body irradiation at four-day intervals up to a total dose of 18 Gy. After each dose per fraction half of the animals were injected with 10(6) bone marrow cells. At four-day intervals evaluations were made of the blood count, bone marrow and spleen cellularities, and spleen mass. In animals subjected only to irradiation the damage to hemopoietic organs was becoming deeper until the end of observation. In bone marrow recipients the decrease in bone marrow cellularity and the number of leukocytes stopped after day 16, and there was a steep growth of splenic cellularity and mass; the decrease in erythrocyte count stopped on day 20. Obviously a certain recovery of hemopoiesis occurred after the total dose of 12 Gy between days 12 and 16. The lodging of injected hemopoietic stem cells and their proliferation had to be preceded by a regeneration of the hemopoietic microenvironment.
Tkadlecek
L
L
Institute of Biophysics, Czechoslovak Academy of Sciences, Brno.
Viklická
S
S
Hofer
M
M
Karpfel
Z
Z
D000818:Animals; D001772:Blood Cell Count; D001773:Blood Cells / Q000528:radiation effects; D016026:Bone Marrow Transplantation; D003037:Cobalt Radioisotopes / Q000008:administration & dosage / Q000009:adverse effects; D004307:Dose-Response Relationship, Radiation; D006410:Hematopoiesis / Q000528:radiation effects; D006413:Hematopoietic System / Q000528:radiation effects*; D051379:Mice; D011829:Radiation Dosage; D011833:Radiation Injuries, Experimental / Q000628:therapy*; D014916:Whole-Body Irradiation
D003037:Cobalt Radioisotopes