A convenient procedure for rapid release of protoplasts from Ustilago maydis.
Filtrates from cultures of Trichoderma harzianum, grown on a liquid mineral salts medium containing vitamin and trace element supplements, 0.5 g/l glucose and 2 g/l of dried residue of ethanol-extracted commercial mushrooms (Agaricus brunnescens), are a good source of enzymes for the release of protoplasts from the sporidia of Ustilago maydis. When concentrated 50-fold by (NH4)2SO4 precipitation, the enzymes liberated 95% of the protoplasts from the sporidia within 40 min.
Waterfield
W F
WF
Dept. of Botany, University of Maryland, College Park 20742.
Sisler
H D
HD
D001487:Basidiomycota / Q000648:ultrastructure*; D002458:Cell Fractionation / Q000379:methods*; D003904:Mitosporic Fungi / Q000201:enzymology*; D011523:Protoplasts / Q000648:ultrastructure*; D014242:Trichoderma / Q000201:enzymology*; D014588:Ustilago / Q000648:ultrastructure*