Suppression of in vitro production of anti-U1-ribonucleoprotein antibody by monoclonal anti-idiotypic antibody to anti-U1-ribonucleoprotein antibody.
We produced monoclonal anti-idiotypic antibody against immunoaffinity purified anti-U1-ribonucleoprotein (RNP) antibody from a patient (K.T.), by the cell fusion procedure. The specificity of monoclonal anti-idiotypic antibody (IgG1, kappa) was determined by inhibition studies. With the monoclonal anti-idiotypic antibody, cross-reactive idiotypes on anti-U1-RNP antibodies from unrelated patients with anti-U1-RNP antibody was detected in 57% of the samples. The anti-idiotypic antibody specifically suppressed the in vitro production of anti-U1-RNP antibody by lymphocytes from the patient K.T., and unrelated patients with a cross-reactive idiotype, in whom idiotype-reactive T cells were demonstrated. The results indicate that anti-idiotypic antibody may modulate the regulation of in vitro anti-U1-RNP antibody production.
Koide
J
J
Second Department of Internal Medicine, Saitama Medical School, Japan.
Takeuchi
T
T
Hosono
O
O
Takano
M
M
Abe
T
T
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D000974:Antibodies, Antinuclear / Q000096:biosynthesis*; D000911:Antibodies, Monoclonal / Q000502:physiology*; D000918:Antibody Specificity; D001202:Ascitic Fluid / Q000378:metabolism; D001402:B-Lymphocytes / Q000276:immunology; D001666:Binding Sites, Antibody; D003429:Cross Reactions; D005260:Female; D006801:Humans; D006825:Hybridomas / Q000378:metabolism; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D007166:Immunosuppressive Agents / Q000502:physiology*; D008214:Lymphocytes / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D012342:RNA, Small Nuclear / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology
D000888:Antibodies, Anti-Idiotypic; D000974:Antibodies, Antinuclear; D000911:Antibodies, Monoclonal; D007130:Immunoglobulin Idiotypes; D007166:Immunosuppressive Agents; D012342:RNA, Small Nuclear