title: Cloning of the Escherichia coli K-12 hemB gene.
abstract: An Escherichia coli heme-requiring, heme-permeable mutant had no detectable 5-aminolevulinate dehydratase or porphobilinogen deaminase activities. The gene which complemented this mutation was cloned to a high-copy-number plasmid, and porphobilinogen deaminase activity was restored to normal levels, but the synthesis of 5-aminolevulinate dehydratase increased 20- to 30-fold. A maxicell procedure confirmed that the gene cloned was hemB.
authors.affiliation: Department of Biochemistry, City College, City University of New York, New York 10031.
authors.forename: J M
authors.initials: JM
authors.lastname: Li
authors.forename: H
authors.initials: H
authors.lastname: Umanoff
authors.forename: R
authors.initials: R
authors.lastname: Proenca
authors.forename: C S
authors.initials: CS
authors.lastname: Russell
authors.forename: S D
authors.initials: SD
authors.lastname: Cosloy
mesh_terms: D000483:Alleles; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005798:Genes, Bacterial; D006418:Heme / Q000096:biosynthesis / Q000235:genetics*; D011163:Hydroxymethylbilane Synthase / Q000096:biosynthesis / Q000235:genetics; D009154:Mutation; D010957:Plasmids; D000623:Porphobilinogen Synthase / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D006418:Heme; D011163:Hydroxymethylbilane Synthase; D000623:Porphobilinogen Synthase