title: Optimization of ligation reaction conditions in gene synthesis.
abstract: Several phosphorylation, annealing and ligation reaction conditions have been investigated for the enzymatic assembly of genes of interest. The use of longer oligodeoxyribonucleotides (40-60 bases long) also improved the enzymatic reaction. Furthermore, the use of longer oligonucleotides and the elimination of long stretches of G's or C's lowered the mutation rate.
authors.affiliation: Biopolymer Chemistry, Upjohn Company, Kalamazoo, MI 49001.
authors.forename: N Y
authors.initials: NY
authors.lastname: Theriault
authors.forename: J B
authors.initials: JB
authors.lastname: Carter
authors.forename: S P
authors.initials: SP
authors.lastname: Pulaski
mesh_terms: D008026:Ligation / Q000379:methods; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis*; D010766:Phosphorylation
chemical_list: D009838:Oligodeoxyribonucleotides