Des-Leu angiotensin I: biosynthesis and drinking response.
The crude rat and bovine synaptosomal lysate from brain can hydrolyze angiotensin I (AI) to des-Leu angiotensin I (AI-dL) and no further. This cytosolic enzyme has a specificity for angiotensin-related sequences, R-His-Pro-Phe-His-Leu and therefore named angiotensin-related carboxypeptidase (ARC). These studies led to the biosynthesis and purification of AI-dL in order to determine if it can provoke a drinking response. This nonapeptide is a potent dipsogen when injected into the cerebroventricles of rats. The drinking response probably requires a second hydrolysis to angiotensin II (AII) since both captopril and saralasin can inhibit this response.
Changaris
D G
DG
University of Louisville School of Medicine, Health Sciences Center, KY 40292.
Porter
J L
JL
Miller
J J
JJ
Levy
R S
RS
D000803:Angiotensin I / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D000804:Angiotensin II / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000378:metabolism; D002216:Captopril / Q000494:pharmacology; D002268:Carboxypeptidases / Q000378:metabolism; D002417:Cattle; D004326:Drinking / Q000187:drug effects*; D066298:In Vitro Techniques; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012504:Saralasin / Q000494:pharmacology; D013574:Synaptosomes / Q000378:metabolism
D000804:Angiotensin II; C045857:angiotensin I, des-Leu(10)-; D000803:Angiotensin I; D002216:Captopril; D002268:Carboxypeptidases; C055883:angiotensin-related carboxypeptidase; D012504:Saralasin