title: [Detection of HIV-1 antigen--determination using 3 different test systems].
abstract: 51 human sera containing antibodies to human immunodeficiency virus 1 (= HIV-1) were examined for HIV-1-antigen by three different enzyme immunoassay procedures (= EIA) of Abbott, Organon and Dupont. Sensibilities, handling as well as the correlation with the clinical stages of HIV-infection were compared. The EIA's diagnosed in accordance 6 sera which contained HIV-1-antigen and 42 sera to be HIV-1-antigen negative. 3 sera showed differences: according to the EIA of Organon none of these sera contained HIV-1-antigen, the EIA of Abbott (but not of Dupont) analysed HIV-1-antigen in one of these sera, in the other two sera only the EIA of Dupont showed HIV-1-Antigen. It is concluded that the differences in these 3 serum samples may originate not only in the different types of EIA used (indirect/direct procedure) but also in the different capture antibodies provided (antibodies against p-24 antigen or polyvalent antibodies).
authors.affiliation: Institut für Medizinische Mikrobiologie und Hygiene, Klinikum der Stadt Mannheim, Mannheim.
authors.forename: F
authors.initials: F
authors.lastname: Düzgün
authors.forename: N
authors.initials: N
authors.lastname: Dickgiesser
authors.forename: D
authors.initials: D
authors.lastname: Schuster
authors.forename: E
authors.initials: E
authors.lastname: Lengfelder
authors.forename: J
authors.initials: J
authors.lastname: Teuber
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology*; D000914:Antibodies, Viral / Q000032:analysis; D000956:Antigens, Viral / Q000032:analysis*; D006678:HIV / Q000276:immunology*; D015483:HIV Antibodies; D015488:HIV Antigens; D006801:Humans; D007124:Immunoenzyme Techniques
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D015483:HIV Antibodies; D015488:HIV Antigens
vernacular_title: Der Nachweis von HIV-1-Antigen--Bestimmung mit Hilfe von drei verschiedenen Testsystemen.