[Antibodies to the structural and nonstructural gag-coded proteins of type-D retroviruses in patients with lymphadenopathy].
Screening of antibodies to structural and nonstructural gag gene-coded proteins in humans with lymphadenopathy and AIDS was performed by means of radioimmunoprecipitation (RIP) and western blotting. Pr78gag precursor of gag-coded proteins of type-D retrovirus from Hep-2 cells served as an antigen in RIP tests. Total number of sera (of humans with lymphoadenopathy) under RIP analysis was 18 and one sera of AIDS patient. Six of them reacted with Pr78gag and one out of one AIDS serum. Over 80 sera samples of humans with lymphadenopathy have been tested by means of western blotting with proteins of Mason-Pfizer monkey virus as antigens. Antibodies to p27 (major internal protein of Mason-Pfizer monkey virus) were detected in 12 sera samples of those with lymphadenopathy (dilution 1:100) and in 9 out of 12 sera of AIDS patients (dilution 1:100-1:400). Results obtained make it possible to predict that type-D retroviruses are associated with acquired immunodeficiency syndrome and generalized lymphadenopathy and could play some role in development of this illness in humans.
Morozov
V A
VA
Il'inskiĭ
P O
PO
Il'in
K V
KV
D000386:AIDS-Related Complex / Q000276:immunology*; D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology; D000914:Antibodies, Viral / Q000032:analysis*; D015153:Blotting, Western / Q000379:methods; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D015683:Gene Products, gag; D006801:Humans; D011233:Precipitin Tests / Q000379:methods; D011863:Radioimmunoassay / Q000379:methods; D012190:Retroviridae / Q000276:immunology*; D012191:Retroviridae Proteins / Q000276:immunology*; D015302:Simian Immunodeficiency Virus / Q000276:immunology; D014764:Viral Proteins / Q000276:immunology*; D015678:Viral Structural Proteins
D000914:Antibodies, Viral; D015683:Gene Products, gag; D012191:Retroviridae Proteins; D014764:Viral Proteins; D015678:Viral Structural Proteins
Antitela k strukturnym i nestrukturnym gag-kodiruemym belkam retrovirusov tipa D u bol'nykh limfoadenopatiiami.