title: Circular intermediates with missing nucleotides in the conversion of supercoiled or nicked circular to linear duplex DNA catalyzed by two species of BAL 31 nuclease.
abstract: The extracellular nucleases from Alteromonas espejiana BAL 31 can catalyze the endonucleolytic and/or exonucleolytic hydrolysis of duplex DNA in response to a variety of alterations, either covalent or noncovalent, in DNA structure. The nuclease can exist as at least two kinetically and molecularly distinct protein species. The two species that have been studied, called the 'fast' (F) and 'slow' (S) nucleases, both readily convert negatively supercoiled DNAs to linear duplex molecules and accomplish this conversion through the formation of a circular duplex intermediate containing usually a single interruption in one strand. It is further shown that most of these intermediates contain gaps arising from the removal in a processive manner of one or more nucleotide residues after the introduction of the initial strand break (nick). Considering only the intermediates with gaps, the average number of missing residues is 6.3 +/- 0.5 and 2.8 +/- 0.3, respectively, for DNA acted upon by the F and S enzymes independently of the extent of conversion of supercoiled DNA. The nicks and gaps are bounded by 3'-hydroxyl and 5'-phosphoryl termini. When singly nicked circular DNA is used as the substrate, conversion to the linear duplex form occurs predominantly through a gapped circular intermediate with the same average numbers, within experimental error, of missing nucleotides for the respective nuclease species as found when supercoiled DNA is the substrate. The conversion to linear duplex DNA is much slower when nicked circular DNA is the substrate compared to that found when supercoiled DNA is the starting material.
authors.affiliation: Department of Biochemical and Biophysical Sciences, University of Houston, TX 77004.
authors.forename: A K
authors.initials: AK
authors.lastname: Przykorska
authors.forename: C R
authors.initials: CR
authors.lastname: Hauser
authors.forename: H B
authors.initials: HB
authors.lastname: Gray
mesh_terms: D004270:DNA, Circular / Q000235:genetics*; D004278:DNA, Superhelical / Q000235:genetics*; D004706:Endodeoxyribonucleases / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics; D007527:Isoenzymes / Q000378:metabolism*; D007700:Kinetics; D010957:Plasmids
chemical_list: D004270:DNA, Circular; D004278:DNA, Superhelical; D007527:Isoenzymes; D004706:Endodeoxyribonucleases; C038407:exonuclease Bal 31