Reduction of adipose tissue lipoprotein lipase activity as a result of in vivo administration of 2,3,7,8-tetrachlorodibenzo-p-dioxin to the guinea pig.
Within 1 hr of intraperitoneal administration of 1 microgram 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)/kg, lipoprotein lipase (LPL) activity was reduced 38% from initial levels in the adipose tissue of the guinea pig. Maximal depression was observed after 2 days and persisted throughout the 10-day observation period. Oral administration of glucose restored LPL activity in TCDD-treated animals after 1 day but only partially after 2 and 5 days, and had no effect after 10 days of exposure. Although initial (2-day) serum insulin levels were depressed, the inability of glucose to restore LPL activity after prolonged exposure was not due to malabsorption of glucose nor to changes in serum thyroxine or insulin concentration. TCDD also inhibited the lipolytic pathway in the adipocyte, but had no effect on hormone sensitive lipase (HSL). Since HSL and LPL are reciprocally regulated, it was concluded that TCDD acts on the adipocyte to uncouple HSL-LPL reciprocity as well as to reduce LPL production.
Brewster
D W
DW
Pesticide Research Center, Michigan State University, East Lansing 48824.
Matsumura
F
F
D000273:Adipose Tissue / Q000187:drug effects* / Q000201:enzymology; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D004147:Dioxins / Q000633:toxicity*; D005947:Glucose / Q000494:pharmacology; D006168:Guinea Pigs; D007328:Insulin / Q000097:blood; D008071:Lipoprotein Lipase / Q000037:antagonists & inhibitors*; D008297:Male; D000072317:Polychlorinated Dibenzodioxins / Q000633:toxicity*; D011494:Protein Kinases / Q000032:analysis; D013974:Thyroxine / Q000097:blood
D004147:Dioxins; D007328:Insulin; D000072317:Polychlorinated Dibenzodioxins; D011494:Protein Kinases; D008071:Lipoprotein Lipase; D005947:Glucose; D013974:Thyroxine