The effect of interleukin 1 beta on proteoglycans synthesized by human gingival fibroblasts in vitro.
The effect of recombinant interleukin-1 beta (IL-1 beta) on proteoglycan synthesis by human gingival fibroblasts was investigated. IL-1 beta stimulated the gingival fibroblasts to proliferate. When compared to human foreskin fibroblasts, the gingival fibroblasts demonstrated a greater proliferative response at higher concentrations of IL-1 beta. The midpoint of the proliferation response for both cell types was in the 10(-11) M IL-1 beta range. The rate of [35S]-sulfate incorporation into proteoglycans by human gingival fibroblasts was enhanced by 40% at 10(-9) M IL-1 beta. This stimulatory effect appeared to be independent of cell proliferation and prostaglandin synthesis since blocking of these functions with hydroxyurea and indomethacin respectively, resulted in similar dose responses to IL-1 beta. Pulse chase experiments indicated the kinetics of degradation in the presence or absence of IL-1 beta were essentially identical. Therefore, the turnover rate of proteoglycans was not altered by IL-1 beta, no significant differences between molecular species, size or glycosaminoglycan composition of the proteoglycans synthesized in the presence or absence of IL-1 beta was noted. Thus, IL-1 beta can modulate extracellular matrix synthesis by human gingival fibroblasts and may therefore be partially responsible for the early events of healing following inflammatory episodes.
Bartold
P M
PM
Department of Pathology, University of Adelaide, Australia.
D002455:Cell Division / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D005881:Gingiva / Q000166:cytology; D006025:Glycosaminoglycans / Q000032:analysis; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology*; D008297:Male; D010413:Penis / Q000166:cytology; D011509:Proteoglycans / Q000032:analysis / Q000096:biosynthesis*; D013431:Sulfates / Q000378:metabolism; D013462:Sulfur Radioisotopes; D013997:Time Factors
D006025:Glycosaminoglycans; D007375:Interleukin-1; D011509:Proteoglycans; D013431:Sulfates; D013462:Sulfur Radioisotopes