Altered interleukin production during Friend leukemia virus infection.
Spleen cells from BALB/c mice, infected 14 to 28 days earlier with Friend leukemia virus (FLV), were shown to be inhibited in their ability to produce interleukin 2 (IL-2) when stimulated with mitogen. Likewise, these spleen cell populations failed to respond following mitogenic stimulation or exogenous addition of recombinant IL-2. By contrast, the FLV-infected spleen cell populations produced normal levels of interleukin 1 (IL-1) and thymocytes from FLV-infected mice responded normally to addition of exogenous IL-1. This suggests that FLV infection selectively affects the ability of spleen cells to produce cytokines. Spleen cell populations enriched for T lymphocytes and depleted of tumor cells by density gradient centrifugation in Ficoll were unable to produce IL-2. This indicates that the failure to detect IL-2 in cells from FLV-infected mice was not due to a dilution of T lymphocytes by tumor cells but was a functional inability to produce IL-2. Furthermore, enriched T lymphocytes from FLV-infected mice failed to respond blastogenically to exogenous IL-2. Additional studies indicate that tumor cells, but not macrophages or T lymphocytes from FLV-infected spleens, suppressed the blastogenic response to mitogens and IL-2 production by normal splenic T lymphocytes.
Lopez-Cepero
M
M
Department of Medical Microbiology and Immunology, University of South Florida, College of Medicine, Tampa 33612.
Specter
S
S
Matteucci
D
D
Friedman
H
H
Bendinelli
M
M
D000818:Animals; D002469:Cell Separation; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D004926:Escherichia coli; D005260:Female; D005622:Friend murine leukemia virus; D007375:Interleukin-1 / Q000096:biosynthesis* / Q000494:pharmacology; D007376:Interleukin-2 / Q000096:biosynthesis*; D007942:Leukemia, Experimental / Q000378:metabolism* / Q000473:pathology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D010835:Phytohemagglutinins / Q000494:pharmacology; D011994:Recombinant Proteins / Q000494:pharmacology; D013154:Spleen / Q000378:metabolism / Q000473:pathology; D013601:T-Lymphocytes / Q000378:metabolism
D007375:Interleukin-1; D007376:Interleukin-2; D008070:Lipopolysaccharides; D010835:Phytohemagglutinins; D011994:Recombinant Proteins