Mitogen plus interleukin 4 induction of C epsilon transcripts in B lymphoid cells.
To elucidate the mechanism of IL-4-induced enhancement of IgE and IgG1 production, murine splenic B cells and A-MuLV-transformed cells were cultured with LPS and IL-4 and assayed for epsilon and gamma 1 transcripts. Concomitant treatment with IL-4 and LPS induced expression of C epsilon transcripts in both normal and transformed cells. Expression of these truncated C epsilon transcripts preceded accumulation of normal epsilon mRNA in treated cells. Consistent data were obtained with respect to gamma 1 RNA expression. These results suggest that IL-4 can direct class switching in the context of a mechanism associated with differential expression of germline constant region genes.
Rothman
P
P
Howard Hughes Medical Institute, New York, New York.
Lutzker
S
S
Cook
W
W
Coffman
R
R
Alt
F W
FW
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D002461:Cell Line, Transformed; D005786:Gene Expression Regulation; D005803:Genes, Immunoglobulin; D007132:Immunoglobulin Isotypes / Q000032:analysis*; D015847:Interleukin-4; D007378:Interleukins / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D051379:Mice
D007132:Immunoglobulin Isotypes; D007378:Interleukins; D008070:Lipopolysaccharides; D015847:Interleukin-4