title: Transverse alternating field electrophoresis (TAFE).
abstract: The rapid development of electrophoretic technology during the past five years has given us a stream of improvements in the area of pulsed-field separation techniques. This progression has culminated in the conception of TAFE, a simple, high resolution technique providing straight lane geometry and separation of fragments up to 9 million base pairs in length. Pulsed-field techniques will unquestionably play a major role in the forthcoming analysis of the human genome by facilitating restriction mapping and cloning of large fragments. We have no doubt that TAFE will allow scientists to do this type of genetic analysis, faster and better than ever before.
authors.affiliation: Beckman Instruments, Inc., Spinco Division, Palo Alto, CA 94304.
authors.forename: G
authors.initials: G
authors.lastname: Stewart
authors.forename: A
authors.initials: A
authors.lastname: Furst
authors.forename: N
authors.initials: N
authors.lastname: Avdalovic
mesh_terms: D015139:Blotting, Southern; D004586:Electrophoresis / Q000295:instrumentation* / Q000379:methods; D011673:Pulsatile Flow; D015183:Restriction Mapping; D012440:Saccharomyces / Q000235:genetics