Influence of class I H-2 gene expression on local tumor growth. Description of a model obtained from clones derived from a solid BALB/c tumor.
A BALB/c methylcholanthrene-induced tumor was studied for class I and II H-2 expression. GR9 was adapted to tissue culture without any passage in vivo to avoid immunoselection mechanisms. Forty-three clones were obtained and typed for H-2 antigens. A variety of techniques were used for typing, for example, 51Cr release assay, radiobinding assay; absorption with anti H-2 monoclonal antibodies or H-2 allo-antisera and alloreacting cytotoxic T lymphocytes. A heterogeneity of H-2 expression was detected in these clones with clones H-2 K+/D+, to clones H-2K-D-. This heterogeneity was also evident when several GR9 clones were tested for in vivo growth in syngeneic BALB/c mice. Two clones, A7 and B9, were selected for further studies and injected into syngeneic animals to measure local tumor growth. A7 (K+/D+) was rather immunogenic while B9 (K-/D-) was not. These differences were probably due to an immunoresponse, since both clones grow similarly in irradiated syngeneic BALB/c mice. Immunoprecipitations of H-2 antigens and SDS-PAGE analysis confirmed the results obtained in serology. Three isoenzymes were also determined in GR9 clones and their electrophoretic mobility was always identical to that obtained in normal BALB/c tissues. These results suggest that K and D molecules are important structures for an immunoresponse against tumor-associated antigens.
Garrido
A
A
Servicio Análisis Clínicos e Inmunología, CS Virgen de las Nieves, Granada, España.
Pérez
M
M
Delgado
C
C
Garrido
M L
ML
Rojano
J
J
Algarra
I
I
Garrido
F
F
D000818:Animals; D000951:Antigens, Neoplasm / Q000096:biosynthesis* / Q000235:genetics; D002999:Clone Cells / Q000276:immunology / Q000473:pathology; D015972:Gene Expression Regulation, Neoplastic; D006085:Graft Survival / Q000276:immunology / Q000528:radiation effects; D006183:H-2 Antigens / Q000096:biosynthesis* / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C / Q000235:genetics; D009374:Neoplasms, Experimental / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D014407:Tumor Cells, Cultured / Q000276:immunology / Q000473:pathology / Q000637:transplantation
D000951:Antigens, Neoplasm; D006183:H-2 Antigens