[Expression of gag gene of human immunodeficiency virus in recombinant vaccinia virus].
A fragment of HTLV-IIIB gag-gene, coding for the first 441 amino acids of the p53 gag-precursor was expressed in the recombinant vaccinia virus, vC5. Two HIV specific proteins were detected by western blot in CV-1 cells infected with vC5. Their relative molecular masses were 50 and 35 Kd, pointing out that the first of the proteins is a full length expression product of the cloned sequence, while the second one is a result of processing or abortive translation. Possibilities of using such a strain as a vaccine or in Western blot conformation test are discussed.
Popov
S A
SA
Barsov
E V
EV
Bukrinskiĭ
M I
MI
Zhdanov
V M
VM
D005786:Gene Expression Regulation; D015683:Gene Products, gag; D005814:Genes, Viral; D006678:HIV / Q000235:genetics* / Q000276:immunology; D015488:HIV Antigens / Q000235:genetics; D010957:Plasmids; D011995:Recombination, Genetic; D012191:Retroviridae Proteins / Q000235:genetics*; D014616:Vaccinia virus / Q000235:genetics*
D015683:Gene Products, gag; D015488:HIV Antigens; D012191:Retroviridae Proteins
Ekspressiia gena gag virusa immunodefitsita cheloveka v sostave rekombinantnogo virusa ospovaktsiny.