Combinations of monoclonal antibodies distinguish mesenchymal, myogenic, and chondrogenic precursors of the developing chick embryo.
Monoclonal antibodies (MAbs) were used as probes for molecular differences in the surfaces of nonterminally differentiated cells of the developing chick limb. The specificity of the MAbs was determined by immunofluorescent localization performed on cultured breast muscle and limb bud cells and cryosections of a variety of embryonic (stages 15-37) and neonatal tissues. Subpopulations of MAb-positive and -negative cells were isolated by fluorescence-activated cell sorting and their developmental potential was assessed in vitro. Cells of the compacted somite, lateral plate mesoderm, and early limb bud were labeled with the CSAT MAb. Myogenic precursors of the dermatome and limb bud were labeled with the CSAT and L4 MAbs. Chondrogenic precursors of the sclerotome and limb bud were labeled with the CSAT, L4, and C5 MAbs. These precursors were distinguished from fibroblasts which were labeled with the CSAT and C1 MAbs. The differentiation and maturation of muscle and cartilage were accompanied by alterations in the labeling patterns of the MAbs. These results indicate that combinations of these MAbs can be used to distinguish mesenchymal, myogenic, and chondrogenic precursors, identify their site of origin during development, and isolate subpopulations of embryonic cells.
George-Weinstein
M
M
Department of Biochemistry, School of Medicine, University of Pennsylvania, Philadelphia 19104.
Decker
C
C
Horwitz
A
A
D000818:Animals; D000911:Antibodies, Monoclonal; D002356:Cartilage / Q000196:embryology*; D002478:Cells, Cultured; D002642:Chick Embryo; D005121:Extremities / Q000196:embryology; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D009132:Muscles / Q000196:embryology*
D000911:Antibodies, Monoclonal