Cell killing and mutagenesis by alkylating agents and UV irradiation in wild-type and deoxycytidine-kinase-deficient Friend murine leukaemia cells.
Wild-type (clone 707) Friend murine leukaemia cells were compared with two ara-C-resistant subclones in terms of sensitivity to cell killing and mutagenesis to 6-thioguanine resistance following treatment with ethyl methane sulphonate, methyl methane sulphonate and UV irradiation. The ara-C-resistant subclones, 707DKE and 707DK48, had respective deoxycytidine kinase activities of 6.7 and 5.4% the values found in wild-type cells. No clear pattern of altered sensitivity to cell killing or mutagenesis emerged between the wild-type cells and the ara-C-resistant subclones. These results do not provide evidence for a role of deoxycytidine kinase in determining sensitivity to mutagenic agents in the Friend cell line.
McKenna
P G
PG
Biomedical Sciences Research Centre, University of Ulster, Coleraine, UK.
Boullier
B A
BA
D000818:Animals; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D002999:Clone Cells; D003561:Cytarabine / Q000494:pharmacology; D004351:Drug Resistance; D005020:Ethyl Methanesulfonate / Q000633:toxicity*; D007942:Leukemia, Experimental / Q000473:pathology*; D008741:Methyl Methanesulfonate / Q000633:toxicity*; D051379:Mice; D009152:Mutagenicity Tests / Q000379:methods; D009154:Mutation; D014466:Ultraviolet Rays
D003561:Cytarabine; D005020:Ethyl Methanesulfonate; D008741:Methyl Methanesulfonate