Concanavalin A-induced splenocyte supernatant causes a preferential increase in H-2K over H-2D and vice versa depending on culture conditions.
Mouse embryo fibroblasts (MEF) from BALB/c mice were exposed to the culture supernatant (CS) from concanavalin A-stimulated splenocytes for 16 h. This caused an increase in the amount of Kd and Dd antigens expressed on the cell surface of the MEF. However, on MEF cultures at 37 degrees C and subcultured daily for the first 5 days after embryonic trypsinization, CS caused the K antigens to increase significantly more than the D antigens, while on MEF cultured for 24 days with a combination of 37 degrees C and room temperature, CS invariably caused the D antigens to increase more than the K antigens. In another protocol involving MEF culture at 37 degrees C and subculture every 4 days, either response was possible when the MEF were exposed to CS. We propose that cells may differentially control K and D antigen increase and discuss how this may be useful in the eradication of viral infection in the animal.
King
N J
NJ
Department of Microbiology, John Curtin School of Medical Research, Australian National University, Canberra, ACT.
Sinickas
V G
VG
Blanden
R V
RV
D000818:Animals; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D003470:Culture Media; D005347:Fibroblasts / Q000276:immunology*; D005786:Gene Expression Regulation / Q000276:immunology; D006183:H-2 Antigens / Q000096:biosynthesis*; D064532:Histocompatibility Antigen H-2D; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000166:cytology / Q000276:immunology*
D003470:Culture Media; D006183:H-2 Antigens; C024755:H-2K(K) antigen; D064532:Histocompatibility Antigen H-2D; D003208:Concanavalin A