title: Effects of leukocidin from Fusobacterium necrophorum on bovine peripheral leukocytes in vitro.
abstract: Partially purified leukocidin from Fusobacterium necrophorum damaged bovine peripheral leukocytes as demonstrated by trypan blue staining. Granulocytes were most and T-lymphocytes least sensitive to the leukocidin. Heating for 30 min at 60 degrees C completely inactivated the leukocidin. The cytotoxicity of the leukocidin could be neutralized by homologous anti-leukocidin. Scanning electron microscopy of the exposed cells revealed an apparent destruction of the cell membranes, loss of the microvilli and smoothing of the cell surfaces.
authors.affiliation: Department of Veterinary, Microbiology, Faculty of Agriculture, Yamaguchi University, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Kanoe
authors.forename: T
authors.initials: T
authors.lastname: Yamamoto
authors.forename: K
authors.initials: K
authors.lastname: Kai
authors.forename: H
authors.initials: H
authors.lastname: Blobel
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000187:drug effects* / Q000648:ultrastructure; D002417:Cattle; D002470:Cell Survival / Q000187:drug effects; D005675:Fusobacterium necrophorum / Q000472:pathogenicity*; D006098:Granulocytes / Q000166:cytology / Q000187:drug effects* / Q000648:ultrastructure; D007956:Leukocidins / Q000302:isolation & purification / Q000494:pharmacology*; D008855:Microscopy, Electron, Scanning; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects* / Q000648:ultrastructure
chemical_list: D007956:Leukocidins