Localization of protein disulfide isomerase on plasma membranes of rat exocrine pancreatic cells.
We investigated immunocytochemically the ultrastructural localization of protein disulfide isomerase (PDI) in rat pancreatic exocrine cells by use of the post-embedding protein A-gold technique. We found that not only the endoplasmic reticulum (ER) and nuclear envelope but also the trans-Golgi cisternae, secretory granules, and plasma membranes were heavily labeled with gold particles. Labeling density of the gold particles in the rough ER and plasma membranes of the exocrine pancreatic cells was twofold and twentyfold greater, respectively, than that of hepatocytes. In the acinar lumen, amorphous material presumably corresponding to the secreted zymogens was also labeled with gold particles. These results suggest that in rat exocrine pancreatic cells a significant amount of PDI is transported to the plasma membrane and secreted to the acinar lumen.
Akagi
S
S
Department of Physiology, Kansai Medical University, Osaka, Japan.
Yamamoto
A
A
Yoshimori
T
T
Masaki
R
R
Ogawa
R
R
Tashiro
Y
Y
D000818:Animals; D002462:Cell Membrane / Q000201:enzymology*; D003594:Cytoplasmic Granules / Q000201:enzymology; D004591:Electrophoresis, Polyacrylamide Gel; D004721:Endoplasmic Reticulum / Q000201:enzymology; D006046:Gold; D006056:Golgi Apparatus / Q000201:enzymology; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D007535:Isomerases / Q000032:analysis*; D008297:Male; D008854:Microscopy, Electron; D009685:Nuclear Envelope / Q000201:enzymology; D010179:Pancreas / Q000201:enzymology* / Q000648:ultrastructure; D019704:Protein Disulfide-Isomerases; D051381:Rats; D011919:Rats, Inbred Strains; D013205:Staphylococcal Protein A
D013205:Staphylococcal Protein A; D006046:Gold; D007535:Isomerases; D019704:Protein Disulfide-Isomerases