In vitro and in vivo DNA interaction with carbonyl-labeled (m)ethylnitrosoureas.
1. The carbonyl group of (m)ethyylnitrosoureas was found to interact with DNA both in vitro and in vivo. 2. (14C-carbonyl)radioactivity remained associated to DNA after a number of ethanolic precipitations as well as after RNase and protein digestion treatments, but was extremely labile to DNA enzymic and acid digestion. 3. Detectable amount of (14C-carbonyl)-radioactivity was still found in DNA of various rat tissues 18 days after i.p. administration of carbonyl-labeled methylnitrosourea.
Kanduc
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D
Dipartimento di Biochimica e Biologia Molecolare, Facoltà di Scienze, Università di Bari, Italy.
Quagliariello
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E
D000818:Animals; D002250:Carbon Radioisotopes; D002417:Cattle; D004247:DNA / Q000378:metabolism*; D005038:Ethylnitrosourea / Q000378:metabolism*; D006868:Hydrolysis; D008297:Male; D008770:Methylnitrosourea / Q000378:metabolism*; D011865:Radioisotope Dilution Technique; D051381:Rats; D011919:Rats, Inbred Strains; D013950:Thymus Gland
D002250:Carbon Radioisotopes; D008770:Methylnitrosourea; D004247:DNA; D005038:Ethylnitrosourea