Mitogenic activities of synthetic Escherichia coli lipid A and a synthetic partial structure (tripalmitoyl pentapeptide) of E. coli lipoprotein.
Synthetic Escherichia coli lipid A and synthetic S-[2,3-bis-(palmitoyloxy)propyl]-N-palmitoylpentapeptide (tripalmitoyl pentapeptide [TPP]), representing the mitogenically active principles of bacterial lipopolysaccharide (LPS) and lipoprotein, respectively, were compared for their mitogenic activities on splenocytes of LPS responder (BALB/c) and LPS-low-responder (C3H/HeJ) mice. Whereas lipid A was active only in LPS-responder mice, TPP resulted in mitogenic activation of B lymphocytes from both LPS-responder and LPS-low-responder mice. When the mitogens were added simultaneously, mainly additive effects of both activators were observed. The data suggest that two different B-lymphocyte populations are responding to these two mitogens.
Brade
L
L
Forschungsinstitut Borstel, Federal Republic of Germany.
Bessler
W G
WG
Brade
H
H
D000818:Animals; D004926:Escherichia coli / Q000276:immunology*; D066298:In Vitro Techniques; D008050:Lipid A / Q000138:chemical synthesis / Q000494:pharmacology*; D055666:Lipopeptides; D008074:Lipoproteins / Q000494:pharmacology*; D008213:Lymphocyte Activation; D051379:Mice; D008815:Mice, Inbred Strains; D008934:Mitogens; D009842:Oligopeptides / Q000494:pharmacology*; D013154:Spleen / Q000166:cytology; D013329:Structure-Activity Relationship
D008050:Lipid A; D055666:Lipopeptides; D008074:Lipoproteins; D008934:Mitogens; D009842:Oligopeptides; C038293:2,3-bis(palmitoyloxy)-2-propyl-N-palmitoyl-cysteinyl-seryl-seryl-asparaginyl-alanine