In vitro production of feline IgG: quantification by an enzyme-linked immunosorbent assay (ELISA).
We report here on the development of a sensitive and convenient enzyme-linked immunosorbent assay (ELISA) for feline IgG by using commercially available reagents and optimizing their concentrations. The reagents employed include goat anti-cat IgG antibody and alkaline phosphatase-conjugated goat anti-cat IgG antibody. The assay described is sensitive, reproducible, and highly specific for feline IgG. The assay was applied for the measurement of feline IgG synthesized and secreted in vitro by peripheral blood mononuclear cells cultured with or without a polyclonal B-cell activator. The amounts of secreted IgG in the supernatants measured by an ELISA correlated well with the numbers of IgG-secreting cells which were induced upon stimulation with pokeweed mitogen and determined by a reverse hemolytic plaque assay.
Mitani
M
M
All Children's Hospital, University of South Florida, St. Petersburg 33701.
Yasuda
M
M
Good
R A
RA
Day
N K
NK
D000818:Animals; D002415:Cats / Q000276:immunology*; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D006462:Hemolytic Plaque Technique; D007074:Immunoglobulin G / Q000032:analysis / Q000096:biosynthesis*; D007958:Leukocyte Count; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D011043:Pokeweed Mitogens / Q000494:pharmacology; D011786:Quality Control
D007074:Immunoglobulin G; D011043:Pokeweed Mitogens