title: Delayed rejection of heart allografts after extracorporeal donor-specific liver hemoperfusion. Role of Kupffer cells.
abstract: Liver allografts have a privileged status in regard to acute rejection. In this experimental study, we have analyzed the immunosuppressive effects of an extracorporeal liver hemoperfusion. In the LEW-to-BN combination of inbred rats, donor-specific liver hemoperfusion can significantly delay acute rejection of heart allografts. Analysis of the immunological status of these animals revealed a significant decrease in donor-specific lymphocytotoxic antibodies and in cytotoxic T lympholysis. Reactivity in mixed lymphocyte culture was normal. After third-party (DA) liver hemoperfusion or after donor-specific (LEW) splenic hemoperfusion, prolongation of heart allograft survival was moderate. Previous blockade of Kupffer cells suppressed the effects of donor-specific liver hemoperfusion. These results suggest that the sequestration by Kupffer cells of a clone of cytotoxic T cells and/or lymphocytotoxic antibodies may explain the immunosuppressive effects of donor-specific liver hemoperfusion.
authors.affiliation: Groupe de Recherche de Chirurgie Hepatique, CNRS ER 277, IRSC, Hospital Paul Brousse, Villejuif, France.
authors.forename: J
authors.initials: J
authors.lastname: Gugenheim
authors.forename: B
authors.initials: B
authors.lastname: Charpentier
authors.forename: M
authors.initials: M
authors.lastname: Gigou
authors.forename: O
authors.initials: O
authors.lastname: Cuomo
authors.forename: F
authors.initials: F
authors.lastname: Calise
authors.forename: L
authors.initials: L
authors.lastname: Amorosa
authors.forename: I
authors.initials: I
authors.lastname: Astarcioglu
authors.forename: M
authors.initials: M
authors.lastname: Trias i Folch
authors.forename: B
authors.initials: B
authors.lastname: Martin
authors.forename: H
authors.initials: H
authors.lastname: Bismuth
mesh_terms: D000818:Animals; D000917:Antibody Formation; D000918:Antibody Specificity; D003602:Cytotoxicity, Immunologic; D006084:Graft Rejection; D006085:Graft Survival; D016027:Heart Transplantation; D006464:Hemoperfusion; D007111:Immunity, Cellular; D007728:Kupffer Cells / Q000502:physiology*; D008099:Liver / Q000276:immunology*; D051381:Rats; D011914:Rats, Inbred BN; D011917:Rats, Inbred Lew; D011919:Rats, Inbred Strains; D014019:Tissue Donors; D014184:Transplantation, Homologous