Adsorption of human beta 2-microglobulin at a water/mercury interface.
Adsorption of human beta 2-microglobulin from a neutral solution of 0.15 M NaCl on a mercury surface was studied at 25 degrees C by measurement of the differential capacity of the electrical double layer. From the diffusion-controlled adsorption kinetics, the surface concentration and hence the area occupied by the adsorbed beta 2-microglobulin molecule were determined at various potentials of the mercury surface. The results indicate unfolding or flattening of beta 2-microglobulin molecules adsorbed in particular on the electrically uncharged surface. The extent of this interfacial conformational rearrangement was reduced with growing positive or negative surface charge density.
Brabec
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Institute of Biophysics, Czechoslovak Academy of Sciences, Brno.
Vucelic
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D
D000327:Adsorption; D001449:Balkan Nephropathy / Q000652:urine; D006801:Humans; D008628:Mercury; D013499:Surface Properties; D014867:Water; D001613:beta 2-Microglobulin / Q000652:urine
D001613:beta 2-Microglobulin; D014867:Water; D008628:Mercury