Ultrastructural identification of type 1 fibres in human skeletal muscle. Immunogold labelling of thin cryosections with a monoclonal antibody against slow myosin.
Existing methods for the ultrastructural identification of fibre types in human skeletal muscle are fallible. This has prompted us to develop a reliable immunoelectron microscopic approach for the identification of human skeletal muscle fibre types. Here we report the unambiguous electron microscopic identification of human type 1 muscle fibres, achieved by combining cryoultramicrotomy with colloidal gold immunocytochemical labelling, using a monoclonal antibody (N0Q7.5.4D) which is specific for the heavy chain of the slow myosin isoform of human skeletal muscle. This method for the identification of muscle fibre types and determination of myosin isoform distributions may have important applications in the ultrastructural study of pathological muscle and in the analysis of myofibrillar assembly during myogenesis.
Semper
A E
AE
Department of Zoology, University of Oxford, U.K.
Fitzsimons
R B
RB
Shotton
D M
DM
D000911:Antibodies, Monoclonal; D005455:Fluorescent Antibody Technique; D006801:Humans; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D009132:Muscles / Q000648:ultrastructure*; D009218:Myosins / Q000032:analysis* / Q000276:immunology
D000911:Antibodies, Monoclonal; D009218:Myosins