title: [The content and composition of gangliosides in brain subcellular fractions of the frog Rana temporaria].
abstract: Ganglioside distribution in various frog brain subcellular fractions (myelin, microsomes, mitochondria, synaptosomes, plasma membranes of nerve endings and synaptic vesicles) was investigated. The synaptosomes and plasma membranes of nerve endings were found to be the main places of ganglioside localization, ganglioside concentration being 2.42 and 1.79 times higher than that in homogenates. Gangliosides were shown to be present in synaptic vesicles. The characteristic features of gangliosides from frog brain and its subcellular fractions are the predominance of polysialogangliosides with 3-5 sialic acid residues (up to 57.4%), low content of monosialogangliosides (not more than 7%) and the presence of disialogangliosides with short carbohydrate chain. The increase of ganglioside content per one nerve cell during phylogenetic development of vertebrates is discussed.
authors.forename: N N
authors.initials: NN
authors.lastname: Nalivaeva
mesh_terms: D000818:Animals; D001923:Brain Chemistry; D002458:Cell Fractionation / Q000379:methods; D002855:Chromatography, Thin Layer; D005732:Gangliosides / Q000032:analysis*; D011896:Rana temporaria / Q000378:metabolism*; D012794:Sialic Acids / Q000032:analysis; D013347:Subcellular Fractions / Q000032:analysis
chemical_list: D005732:Gangliosides; D012794:Sialic Acids
vernacular_title: Soderzhanie i sostav gangliozidov v subkletochnykh fraktsiiakh mozga liagushki Rana temporaria.