Function of a yeast TATA element-binding protein in a mammalian transcription system.
Saccharomyces cerevisiae contains a protein which is functionally similar to the mammalian TATA element-binding transcription factor, TFIID. The yeast factor substitutes for TFIID in a mammalian RNA polymerase II in vitro transcription system, forms a stable preinitiation complex on the Adenovirus-2 major late promoter, and binds specifically to the TATA boxes of the viral promoter and the yeast CYC1 promoter. Interestingly, the yeast factor promotes initiation at a distance from the TATA element typical of a mammalian system.
Buratowski
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Department of Biology, Massachusetts Institute of Technology, Cambridge 02139.
Hahn
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S
Sharp
P A
PA
Guarente
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D004268:DNA-Binding Proteins / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D006367:HeLa Cells / Q000378:metabolism; D006801:Humans; D010442:Peptide Chain Initiation, Translational; D011401:Promoter Regions, Genetic; D012319:RNA Polymerase II / Q000502:physiology; D012045:Regulatory Sequences, Nucleic Acid; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D013045:Species Specificity; D014157:Transcription Factors / Q000302:isolation & purification / Q000378:metabolism / Q000502:physiology*; D014158:Transcription, Genetic
D004268:DNA-Binding Proteins; D014157:Transcription Factors; D012319:RNA Polymerase II