In vitro cytodifferentiation of perinatal rat islet cells within a tridimensional matrix of collagen.
Cell suspensions prepared by collagenase digestion of pancreases obtained from rat fetuses (21.5 d old) and newborns (2.5 d old) were mixed with a collagen solution and inoculated on a collagen base layer. At the onset of the culture, most acinar cells became necrotic, whereas other epithelial cells proliferated. Most of the cell clusters arranged themselves into simple polarized structures composed of epithelial cells forming hollow spheres, and from these budded neoformed endocrine islets. Scarce fibroblasts were located close to these structures. Immunocytochemical localization of insulin and glucagon, as well as ultrastructural characteristics of the cell types revealed an intrainsular distribution similar to the in vivo localization. Tridimensional matrix of collagen offers, to perinatal pancreatic cells in culture, an environment close to the in vivo conditions: cells reorganize themselves in tissuelike structures and cell interactions concerned in the cytodifferentiation of pancreatic islets occur. This system allows for the study of undifferentiated epithelial cells--the presumed stem cells--differentiating and differentiated endocrine cells in the same preparation.
Amory
B
B
Laboratoire de Biologie Cellulaire, Universite Catholique de Louvain, Louvain-la-Neuve, Belgium.
Mourmeaux
J L
JL
Remacle
C
C
D000818:Animals; D002454:Cell Differentiation; D002455:Cell Division; D002478:Cells, Cultured; D003094:Collagen; D003470:Culture Media; D004847:Epithelial Cells; D004848:Epithelium / Q000032:analysis / Q000648:ultrastructure; D005934:Glucagon / Q000032:analysis; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D007328:Insulin / Q000032:analysis; D007515:Islets of Langerhans / Q000032:analysis / Q000166:cytology* / Q000648:ultrastructure; D008854:Microscopy, Electron; D010179:Pancreas / Q000032:analysis / Q000166:cytology* / Q000648:ultrastructure; D051381:Rats; D011919:Rats, Inbred Strains
D003470:Culture Media; D007328:Insulin; D003094:Collagen; D005934:Glucagon