The relationship between induced and spontaneous autoantibodies in MRL mice: the role of Ly-1 B cells?
The murine response to bromelain-treated mouse red blood cells (BrMRBC) is derived from Ly-1 B cells. It has been proposed that this B-cell subset produces a variety of other autoantibodies and is elevated in autoimmune mouse strains. We have studied the ability of MRL lpr/lpr and the non-autoimmune congenic MRL +/+ mice to make autoantibodies to BrMRBC and immunoglobulin (rheumatoid factors, RF). Following lipopolysaccharide (LPS) stimulation we found the numbers of autologous plaque-forming cells (PFC) to be low in both lpr and MRL +/+ mice, suggesting low Ly-1 B-cell numbers. This observation is consistent with the view that Ly-1 B cells in the mouse may not give rise to pathologically relevant RF.
Bond
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Department of Immunology, Middlesex Hospital Medical School, London, U.K.
Hay
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Cooke
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D000818:Animals; D000921:Antibody-Producing Cells / Q000276:immunology; D000950:Antigens, Ly / Q000032:analysis*; D001323:Autoantibodies / Q000096:biosynthesis*; D001327:Autoimmune Diseases / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology*; D004912:Erythrocytes / Q000276:immunology; D006462:Hemolytic Plaque Technique; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D008815:Mice, Inbred Strains; D012217:Rheumatoid Factor / Q000096:biosynthesis; D013154:Spleen / Q000276:immunology
D000950:Antigens, Ly; D001323:Autoantibodies; D008070:Lipopolysaccharides; D012217:Rheumatoid Factor