Antibacterial capacity of buccal epithelial cells from healthy donors and children with recurrent urinary tract infections.
The effect of buccal epithelial cells (BEC) on bacterial growth was investigated in healthy subjects as well as in patients with recurrent urinary tract infections (UTI) and compared to the antibacterial capacity of uroepithelial cells (UEC) of the same individuals. Epithelial cells were obtained from the following groups: healthy female controls; females without further UTI after reflux operation; females with asymptomatic bacteriuria (ABU); females with further UTI despite successful reflux operation; and patients with meningomyelocele (MMC) and recurrent UTI due to significant residual urinary volume. Cocultivation of Escherichia coli with BEC as well as UEC from healthy females or patients with MMC resulted in significant suppression of bacterial growth. However, neither type of epithelial cell showed an antibacterial effect when they were obtained from patients with recurrent UTI in the absence of urological abnormalities (ABU patients; reflux-corrected patients with further UTI). From these results it is concluded that a generalised epithelial defence defect is one important pathogeneic factor for recurrent idiopathic UTI.
Schofer
O
O
Universitäts-Kinderklinik, Mainz, Federal Republic of Germany.
Ludwig
K H
KH
Mannhardt
W
W
Beetz
R
R
Zepp
F
F
Schulte-Wissermann
H
H
D000293:Adolescent; D000328:Adult; D000898:Antibiosis; D001422:Bacterial Adhesion; D002648:Child; D002675:Child, Preschool; D003114:Colony-Forming Units Assay; D004848:Epithelium / Q000382:microbiology; D004926:Escherichia coli / Q000254:growth & development; D005260:Female; D006801:Humans; D009061:Mouth Mucosa / Q000382:microbiology*; D012008:Recurrence; D014551:Urinary Tract / Q000382:microbiology; D014552:Urinary Tract Infections / Q000382:microbiology*