title: ELISA analysis of IgA subclass antibodies to dietary antigens. Elevated IgA1 antibodies in children with coeliac disease.
abstract: Enzyme immunoassays for the quantitation of IgA1 and IgA2 antibodies to dietary antigens were developed. Serum IgA1 antibodies to bovine serum albumin (BSA) were detectable in 2/30 healthy adults, in 3/26 healthy children, and at high levels in 8/11 children with coeliac disease, without relation to gluten exposure. IgA1 antibodies to ovalbumin (OA) and beta-lactoglobulin (BLG) at high titers were seen in one coeliac child but were otherwise low or absent. IgA2 antibodies to BSA were detectable in 28/48 healthy subjects and in 8/11 coeliac children. IgA2 antibodies to OA and BLG were measurable in a few samples from each group. IgA1 antibodies to the gluten component glycgli were found at low levels in 15/56 normal sera, and anti-glycgli antibodies of the IgA2 subclass in 14/48 sera from healthy persons, also at low levels. IgA1 anti-glycgli antibodies were measurable in 5/11 sera from CD patients on a gluten-free diet. Elevated levels of IgA1 anti-glycgli antibodies were detected in all sera from CD patients challenged with gluten, except in 1 patient with a markedly reduced serum IgA level. In contrast, the IgA2 anti-glycgli antibody levels were unaffected. Thus, increased levels of IgA antibodies to dietary protein antigens in childhood coeliac disease were observed only within the IgA1 isotype.
authors.affiliation: Institute of Medical Microbiology, Odense University, Denmark.
authors.forename: M
authors.initials: M
authors.lastname: Kemp
authors.forename: S
authors.initials: S
authors.lastname: Husby
authors.forename: M L
authors.initials: ML
authors.lastname: Larsen
authors.forename: S E
authors.initials: SE
authors.lastname: Svehag
mesh_terms: D000293:Adolescent; D000328:Adult; D000485:Allergens / Q000276:immunology*; D000888:Antibodies, Anti-Idiotypic / Q000032:analysis; D000911:Antibodies, Monoclonal / Q000032:analysis; D000918:Antibody Specificity; D002648:Child; D002675:Child, Preschool; D004044:Dietary Proteins / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005983:Glutens / Q000276:immunology; D006801:Humans; D007070:Immunoglobulin A / Q000096:biosynthesis / Q000145:classification*; D007223:Infant; D007782:Lactoglobulins / Q000276:immunology; D010047:Ovalbumin / Q000276:immunology; D012710:Serum Albumin, Bovine / Q000276:immunology
chemical_list: D000485:Allergens; D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D004044:Dietary Proteins; D007070:Immunoglobulin A; D007782:Lactoglobulins; D012710:Serum Albumin, Bovine; D005983:Glutens; D010047:Ovalbumin