Interleukin-3-specific modification of cell membrane "fluidity" of haemopoietic cells.
The work reported here clearly demonstrates that a specific growth factor, interleukin-3 (IL-3), which acts on multipotent haemopoietic stem cells as well as on committed myeloid progenitor cells of different lineages (Schrader, 1988; Whetton and Dexter, 1986), specifically induces a modification of the physical state ("fluidity") of the cell membranes of two IL-3-responsive and apparently normal haemopoietic cell lines. Furthermore, in a derived IL-3 independent myeloid leukaemic cell line, no such physical response to IL-3 binding was observed. The rapidity of the "normal" response suggests further that it may be associated with, or even constitute per se a critical early effect elicited by IL-3 in sensitive cells, the necessity for which is abrogated in the malignant derivative.
Laskay
G
G
Department of Biophysics, Paterson Institute for Cancer Research, Christie Hospital and Holt Radium Institute, Manchester, United Kingdom.
Dale
R e
Re
Jelínek
J
J
Spooncer
E
E
Dexter
T M
TM
D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000502:physiology; D004161:Diphenylhexatriene; D005454:Fluorescence Polarization; D006412:Hematopoietic Stem Cells / Q000502:physiology* / Q000648:ultrastructure; D007377:Interleukin-3 / Q000502:physiology*; D007700:Kinetics; D008560:Membrane Fluidity / Q000502:physiology*; D051379:Mice
D007377:Interleukin-3; D004161:Diphenylhexatriene