Monoclonal antibodies for radioimmunoassay of cyclosporine: a multicenter comparison of their performance with the Sandoz polyclonal radioimmunoassay kit.
The performance of a radioimmunoassay kit containing monoclonal specific and nonspecific antibodies to cyclosporine (Sandimmun-Kit; Sandoz Ltd., Basle, Switzerland) was compared with that of the original Sandoz polyclonal radioimmunoassay kit (Ciclosporin RIA-Kit). A total of 1320 blood and plasma samples from patients receiving cyclosporine after kidney, heart, liver, and bone-marrow transplantation were analyzed at six centers. For blood samples the median result on using the specific assay was about 50% of the polyclonal assay result after kidney and bone-marrow transplantation, about 33% after heart and liver transplantation; comparable figures for plasma samples were 70 and 40%. The monoclonal nonspecific-antibody assay produced results 10% to 140% higher than polyclonal-assay results, depending on sample matrix and transplant indication; the largest difference was seen in samples from heart- and liver-transplant recipients. Evidently the specific-antibody assay provides a convenient alternative to high-performance liquid chromatography for specific measurement of the drug, but the role of the new nonspecific antibody, possessing an even broader spectrum of cross-reactivity with cyclosporine metabolites than the original polyclonal antiserum, has yet to be defined.
Holt
D W
DW
Poisons Unit, Guy's Hospital, London, U.K.
Johnston
A
A
Marsden
J T
JT
Vernillet
L
L
Keown
P A
PA
Rosano
T G
TG
Shaw
L M
LM
Rosenthaler
J
J
D000906:Antibodies / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D016026:Bone Marrow Transplantation; D002851:Chromatography, High Pressure Liquid; D003524:Cyclosporins / Q000097:blood* / Q000276:immunology / Q000627:therapeutic use; D016027:Heart Transplantation; D006801:Humans; D016030:Kidney Transplantation; D016031:Liver Transplantation; D011863:Radioimmunoassay; D011933:Reagent Kits, Diagnostic; D013223:Statistics as Topic
D000906:Antibodies; D000911:Antibodies, Monoclonal; D003524:Cyclosporins; D011933:Reagent Kits, Diagnostic