Determination of the structure of the Escherichia coli K100 capsular polysaccharide, cross-reactive with the capsule from type b Haemophilus influenzae.
The structure of the Escherichia coli K100 capsular polysaccharide, cross-reactive with that from type b Haemophilus influenzae, was determined by using a combination of chemical and spectroscopic techniques. The structure of the K100 repeating unit was found to be----3)-beta-D-Ribf-(1----2)-D-ribitol-5-(PO4----. The K100 polysaccharide is thus identical in composition to, but different in linkage from, the H. influenzae type b capsular polysaccharide, which has beta-D-Ribf-(1----1)-D-ribitol linkages.
Tsui
F P
FP
Biophysics Laboratory, Food and Drug Administration, Bethesda, Maryland 20892.
Egan
W
W
Summers
M F
MF
Byrd
R A
RA
Schneerson
R
R
Robbins
J B
JB
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D003429:Cross Reactions; D004926:Escherichia coli / Q000276:immunology*; D006193:Haemophilus influenzae / Q000276:immunology*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D011135:Polysaccharides, Bacterial / Q000276:immunology*
D011135:Polysaccharides, Bacterial