[Use of immunoenzyme analysis on nitrocellulose membranes for assessing different methods of purifying the yellow mosaic virus of beans].
The comparative study of different methods for the purification of bean yellow mosaic virus isolated from lupine has been made. To evaluate the method of purification, electron-microscopic, spectrophotometric and ELISA techniques have been used. The possibility of using ELISA on nitrocellulose filters with the utilization of a peroxidase-antiperoxidase complex, manufactured in the USSR, for the determination of viral carriership in plants has been shown.
Molchanets
O V
OV
Taĭkova
N V
NV
Zhmurko
L I
LI
Grashchenko
T B
TB
Boĭko
A L
AL
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D000956:Antigens, Viral / Q000032:analysis; D003101:Collodion; D007887:Fabaceae / Q000382:microbiology; D007124:Immunoenzyme Techniques / Q000295:instrumentation; D008567:Membranes, Artificial; D008854:Microscopy, Electron; D009029:Mosaic Viruses / Q000276:immunology / Q000302:isolation & purification*; D010946:Plants, Medicinal; D011817:Rabbits; D012639:Seeds / Q000382:microbiology; D012692:Serial Passage; D013053:Spectrophotometry
D000914:Antibodies, Viral; D000956:Antigens, Viral; D008567:Membranes, Artificial; D003101:Collodion
Ispol'zovanie immunofermentnogo analiza na nitrotselliuloznykh membranakh dlia otsenki razlichnykh sposobov ochistki virusa zheltoĭ mozaiki fasoli.