The effect of dietary fish oil and long-term salt loading on blood pressure and eicosanoid metabolism in spontaneously hypertensive rats.
1. Dietary suppression of prostanoid synthesis with fish oils has had little effect on blood pressure in models of experimental hypertension in rats. However, a pressor effect of dietary fish oils was observed in spontaneously hypertensive rats (SHR) subject to 1 week of salt loading. 2. Animals were allocated to semisynthetic diets containing either 10% by weight Max EPA fish oil or a control diet of coconut oil, and studied after receiving 1.5% saline for 4 weeks. 3. Within the first week of salt loading, SHR-fed fish oil showed an increase in blood pressure (mean = 9 mmHg) relative to controls. This effect was transient, and after the first week of salt loading there was little difference in blood pressure between the two dietary groups. 4. Following dietary treatment there were substantial changes in plasma fatty acid composition with a 48% decrease in arachidonic acid content of fish oil-fed rats compared with control animals. Rats on the fish oil diet showed a threefold decrease in serum thromboxane generation. Prostacyclin production by incubated segments of aorta was reduced by more than 50% compared with the coconut oil-fed control group. 5. SHR on the fish oil diet showed increased urine volume and sodium excretion, presumably due to increased fluid and salt intake. 6. This study shows that dietary suppression of prostacyclin synthesis is associated with only a minor effect on blood pressure in long-term salt loading of SHR.
Croft
K D
KD
University Department of Medicine, Royal Perth Hospital, Western Australia.
Beilin
L J
LJ
Codde
J
J
Vandongen
R
R
D015121:6-Ketoprostaglandin F1 alpha / Q000097:blood; D000818:Animals; D001794:Blood Pressure / Q000187:drug effects*; D001835:Body Weight / Q000187:drug effects; D002849:Chromatography, Gas; D004032:Diet; D015777:Eicosanoids / Q000378:metabolism*; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005395:Fish Oils / Q000494:pharmacology*; D006973:Hypertension / Q000378:metabolism / Q000503:physiopathology*; D008297:Male; D010972:Platelet Activating Factor / Q000096:biosynthesis; D011188:Potassium / Q000652:urine; D051381:Rats; D011918:Rats, Inbred SHR; D012964:Sodium / Q000494:pharmacology* / Q000652:urine; D013929:Thromboxane B2 / Q000097:blood; D014563:Urodynamics / Q000187:drug effects
D015777:Eicosanoids; D005230:Fatty Acids, Nonesterified; D005395:Fish Oils; D010972:Platelet Activating Factor; D013929:Thromboxane B2; D015121:6-Ketoprostaglandin F1 alpha; D012964:Sodium; D011188:Potassium