title: Experimental depletion of different renal interstitial cell populations.
abstract: To define different populations of renal interstitial cells and investigate some aspects of their function, we studied the kidneys of normal rats and rats with hereditary diabetes insipidus (DI, Brattleboro) after experimental manipulations expected to alter the number of interstitial cells. DI rats showed an almost complete loss of interstitial cells in their renal papillae after treatment with a high dose of vasopressin. In spite of the lack of interstitial cells, the animals concentrated their urine to the same extent as vasopressin-treated normal rats, indicating that the renomedullary interstitial cells do not have an important function in concentrating the urine. The interstitial cells returned nearly to normal within 1 week off vasopressin treatment, suggesting a rapid turnover rate of these cells. To further distinguish different populations of interstitial cells, we studied the distribution of class II MHC antigen expression in the kidneys of normal and bone-marrow depleted Wistar rats. Normal rats had abundant class II antigen-positive interstitial cells in the renal cortex and outer medulla, but not in the inner medulla (papilla). Six days after 1000 rad whole body irradiation, the stainable cells were almost completely lost, but electron microscopic morphometry showed a virtually unchanged volume density of interstitial cells in the cortex and outer medulla, as well as the inner medulla. Thus, irradiation abolished the expression of the class II antigen but caused no significant depletion of interstitial cells.
authors.affiliation: Karolinska Institutet, Department of Pathology, Huddinge Hospital, Sweden.
authors.forename: S O
authors.initials: SO
authors.lastname: Bohman
authors.forename: B
authors.initials: B
authors.lastname: Sundelin
authors.forename: U
authors.initials: U
authors.lastname: Forsum
authors.forename: B
authors.initials: B
authors.lastname: Tribukait
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000528:radiation effects; D003919:Diabetes Insipidus / Q000473:pathology* / Q000503:physiopathology; D006681:HLA-D Antigens / Q000032:analysis; D007671:Kidney Concentrating Ability; D007679:Kidney Medulla / Q000166:cytology* / Q000187:drug effects / Q000473:pathology / Q000502:physiology; D008297:Male; D008854:Microscopy, Electron; D051381:Rats; D011910:Rats, Brattleboro; D011920:Rats, Inbred WF; D014667:Vasopressins / Q000494:pharmacology; D014916:Whole-Body Irradiation
chemical_list: D006681:HLA-D Antigens; D014667:Vasopressins