2-Aminopurine selectively inhibits the induction of beta-interferon, c-fos, and c-myc gene expression.
The protein kinase inhibitor 2-aminopurine (2AP) blocks the induction of the human beta-interferon gene by virus or poly(I)-poly(C) at the level of transcription. This inhibition is specific, since 2AP does not inhibit induction of either the hsp70 heat-shock gene by high temperature or the metallothionein gene by cadmium or dexamethasone. However, 2AP does block the induction of the c-fos and c-myc proto-oncogenes by serum growth factors or virus, suggesting that a protein kinase may be involved in the regulation of these genes, as well as of the beta-interferon gene. However, different factors must be required for the induction of these three genes, since they are not coordinately regulated by the same inducers in most of the cell lines examined.
Zinn
K
K
Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, MA 02138.
Keller
A
A
Whittemore
L A
LA
Maniatis
T
T
D015075:2-Aminopurine / Q000494:pharmacology*; D000225:Adenine / Q000031:analogs & derivatives*; D000818:Animals; D002478:Cells, Cultured; D005786:Gene Expression Regulation / Q000187:drug effects*; D006360:Heat-Shock Proteins / Q000235:genetics; D006801:Humans; D007370:Interferon Type I / Q000235:genetics*; D051379:Mice; D047428:Protein Kinase Inhibitors; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D011519:Proto-Oncogenes; D012045:Regulatory Sequences, Nucleic Acid; D014158:Transcription, Genetic / Q000187:drug effects
D006360:Heat-Shock Proteins; D007370:Interferon Type I; D047428:Protein Kinase Inhibitors; D011518:Proto-Oncogene Proteins; D015075:2-Aminopurine; D000225:Adenine