Detection of activated ras oncogenes in human thyroid carcinomas.
Focus formation following DNA transfection of mouse 3T3-Vill cells was used to search for the presence of activated oncogenes in human thyroid tumors. Oncogenes belonging to the ras family were detected in four out of six thyroid carcinomas (Ki-ras in one anaplastic tumor and one follicular moderately differentiated tumor and Ha-ras and N-ras in two papillary tumors). Normal thyroid tissue samples obtained from two patients, one with an anaplastic tumor and one with a benign adenoma, and samples from 4 benign adenomas and from one toxic goiter of a patient with Graves' disease gave negative results. In one case, restriction enzyme analysis demonstrated the presence of a mutation in codon 12 of the activated Ha-ras oncogene. Our data show that all three ras proto-oncogenes can become activated in malignant thyroid tumors.
Suárez
H G
HG
Institut de Recherches Scientifiques sur le Cancer, Villejuif, France.
Du Villard
J A
JA
Caillou
B
B
Schlumberger
M
M
Tubiana
M
M
Parmentier
C
C
Monier
R
R
D000236:Adenoma / Q000235:genetics*; D000818:Animals; D002277:Carcinoma / Q000235:genetics*; D002460:Cell Line; D004273:DNA, Neoplasm / Q000235:genetics; D005347:Fibroblasts; D005786:Gene Expression Regulation; D006111:Graves Disease / Q000235:genetics; D006801:Humans; D051379:Mice; D009363:Neoplasm Proteins / Q000235:genetics*; D009857:Oncogenes; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D016283:Proto-Oncogene Proteins p21(ras); D013964:Thyroid Neoplasms / Q000235:genetics*; D014162:Transfection
D004273:DNA, Neoplasm; D009363:Neoplasm Proteins; D011518:Proto-Oncogene Proteins; C501009:HRAS protein, human; D016283:Proto-Oncogene Proteins p21(ras)