Modulating activity of B lymphocytes on haemopoiesis (II)--the purification of proliferation amplifying factor (PAF).
In recent years we have done a series of observations on the haemopoietic modulation effects of lymphocytes. Now we report here, for the first time, the purification of a modulating factor (proliferation amplifying factor, PAE) with affinity chromatography and HPLC from supernatant conditioned by a lymphoma cell line M12.4.1. RAF exhibits on SDS-PAGE only one band, the molecular weight is about 31,000, the specific activity is 20,000 times that in the conditioned medium. It may provide a direct evidence that the B lymphocyte also possesses some haemopoietic modulation effects.
Yao
Z J
ZJ
Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing.
Jiang
F Z
FZ
Li
Y
Y
Guo
Y J
YJ
Zhang
M W
MW
Xu
Y J
YJ
Tang
P X
PX
D001402:B-Lymphocytes / Q000502:physiology*; D003114:Colony-Forming Units Assay; D006410:Hematopoiesis; D006801:Humans; D008223:Lymphoma / Q000032:analysis; D011506:Proteins / Q000302:isolation & purification*; D014407:Tumor Cells, Cultured
D011506:Proteins