Z form of poly d(A-T).poly d(A-T) in solution studied by CD and UV spectroscopies.
Near UV CD spectra, UV absorption spectra and their first derivatives have been recorded on poly d(A-T).poly d(A-T) solutions in presence of high NaCl concentration and various amounts of NiCl2. Comparison of the results presented here with those obtained for poly d(G-C).poly d(G-C) and poly d(A-C).poly d(G-T) in comparable conditions, and the I.R. and Raman data on poly d(A-T).poly d(A-T), allows us to assign the new spectra to the Z conformation of poly d(A-T).poly d(A-T) in solution. The mechanism by which nickel ions induce the B----Z interconversion in the presence of high NaCl concentration is discussed.
Bourtayre
P
P
Laboratoire de Spectroscopie Biomoléculaire U.F.R. de Médecine, Université Paris XIII Bobigny, France.
Liquier
J
J
Pizzorni
L
L
Taillandier
E
E
D002942:Circular Dichroism; D004247:DNA / Q000187:drug effects / Q000648:ultrastructure*; D009532:Nickel / Q000494:pharmacology; D009690:Nucleic Acid Conformation / Q000187:drug effects; D011067:Poly dA-dT; D011089:Polydeoxyribonucleotides; D012965:Sodium Chloride / Q000494:pharmacology; D013056:Spectrophotometry, Ultraviolet
D011089:Polydeoxyribonucleotides; D011067:Poly dA-dT; D012965:Sodium Chloride; C022838:nickel chloride; D009532:Nickel; D004247:DNA