title: 13C NMR assignments of the protonated carbons of [d(TAGCGCTA)]2 by two-dimensional proton-detected heteronuclear correlation.
abstract: The resonances of the protonated carbons of [d(TAGCGCTA)]2 have been assigned by the two-dimensional proton-detected double-quantum heteronuclear correlation experiment [( 1H-13C]-DQCOSY). 13C-coupled and 13C-decoupled versions of the experiment were used. The assignment method is discussed in detail. The deoxyribose cross peaks segregate into five well-resolved regions, and the base cross peaks have distinct features that are helpful for assignments. The cross peaks from the 1H-13C pairs at the Cyd5, Ado2 and ThdCH3 base positions fall in separate regions of the spectrum from each other; they also are resolved from the closely spaced Ado8, Guo8, Cyd6 and Thd6. Additional parameters for distinction of the base signals are their differing J-coupling values and long-range coupling patterns.
authors.affiliation: NMR and Data Processing Laboratory NIH Resource, Syracuse University, New York 13244-1200.
authors.forename: S R
authors.initials: SR
authors.lastname: LaPlante
authors.forename: J
authors.initials: J
authors.lastname: Ashcroft
authors.forename: D
authors.initials: D
authors.lastname: Cowburn
authors.forename: G C
authors.initials: GC
authors.lastname: Levy
authors.forename: P N
authors.initials: PN
authors.lastname: Borer
mesh_terms: D002247:Carbon Isotopes; D009682:Magnetic Resonance Spectroscopy; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides
chemical_list: D002247:Carbon Isotopes; D009838:Oligodeoxyribonucleotides