title: Expression of leucocyte antigens by cells from the metrial gland of the pregnant rat.
abstract: The function of the metrial gland of the rat, and particularly of its characteristic population of granulated cells, remains unknown. However, several lines of evidence suggest that the granulated cells may derive from lymphocytes, and play a role in the immunology of pregnancy. In this study, antigen expression by granulated and other cell populations from the metrial glands of rats at Days 13 and 14 of pregnancy was studied by an indirect immunoperoxidase method. Acetone-fixed frozen sections, and cytocentrifuge preparations of collagenase-dispersed metrial gland tissue in which numbers of granulated cells had been increased by density-gradient centrifugation, were used. The primary antibodies used recognised, inter alia, B lymphocytes (MRC OX-3, MRC OX-6, MRC OX-12), T lymphocytes (MRC OX-8, W3/25, MRC OX-19), neutrophils (MRC OX-42) and cells of the monocyte/macrophage series (MRC OX-3, MRC OX-6, MRC OX-42, MRC OX-43). The majority of the granulated cells, including smaller, "immature" forms, were unlabelled by any of these antibodies. Some lymphocytes, and varying numbers of larger, non-granulated cells, were labelled by OX-6, OX-12, W3/25, OX-42 and OX-43. In addition to lymphocytes, labelled cells included neutrophils (OX-42), endothelial cells (OX-43), and probably some macrophages (OX-6, OX-43). OX-12, which recognises the kappa chain of rat IgG, labelled some large cells which may have been stromal cells. These findings do not support the concept that the granulated cells are derived from lymphocytes.
authors.affiliation: Department of Veterinary Preclinical Sciences, University of Melbourne, Parkville, Victoria, Australia.
authors.forename: J D
authors.initials: JD
authors.lastname: O'Shea
authors.forename: M E
authors.initials: ME
authors.lastname: Cerini
authors.forename: H A
authors.initials: HA
authors.lastname: Ward
mesh_terms: D000818:Animals; D005260:Female; D006649:Histocompatibility Antigens / Q000032:analysis*; D007124:Immunoenzyme Techniques; D007962:Leukocytes / Q000166:cytology / Q000276:immunology; D008214:Lymphocytes / Q000166:cytology / Q000276:immunology; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000276:immunology*; D051381:Rats
chemical_list: D006649:Histocompatibility Antigens