Simian retrovirus-D serotype 1 (SRV-1) envelope glycoproteins gp70 and gp20: expression in yeast cells and identification of specific antibodies in sera from monkeys that recovered from SRV-1 infection.
The gp70 and transmembrane gp20 envelope proteins of simian retrovirus-D serotype 1 (SRV-1) were expressed in Saccharomyces cerevisiae as fusion proteins with human superoxide dismutase (SOD). Expression of the SOD-gp70 and SOD-gp20 sequences yielded fusion proteins of 52 and 29 kilodaltons, respectively. The yeast-expressed SRV-1 envelope proteins were used in an enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies in the sera of rhesus macaques that recovered from SRV-1. Sera from 47 of 49 such monkeys tested positive for antibodies to the SOD-gp70 fusion protein, while 45 of 49 reacted positively to SOD-gp20. None of 26 SRV-1-nonexposed monkeys tested positive in either ELISA. Monkeys immunized with the recombinant SRV-1 gp20 and gp70 proteins made good ELISA and Western blot (immunoblot) antibodies to whole SRV-1. This antibody was not neutralizing in vitro, however.
Kwang
H S
HS
Department of Medicine, School of Veterinary Medicine, University of California, Davis 95616.
Barr
P J
PJ
Sabin
E A
EA
Sujipto
S
S
Marx
P A
PA
Power
M D
MD
Bathurst
I C
IC
Pedersen
N C
NC
D000163:Acquired Immunodeficiency Syndrome / Q000662:veterinary*; D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D005786:Gene Expression Regulation; D008253:Macaca mulatta; D008992:Monkey Diseases / Q000382:microbiology*; D010957:Plasmids; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D012190:Retroviridae / Q000276:immunology*; D012191:Retroviridae Proteins / Q000096:biosynthesis / Q000276:immunology*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D014759:Viral Envelope Proteins / Q000096:biosynthesis / Q000276:immunology*
D000914:Antibodies, Viral; D011993:Recombinant Fusion Proteins; D012191:Retroviridae Proteins; D014759:Viral Envelope Proteins