The detection of measles specific immunoglobulin M antibodies using biotinylated antigens.
The methods of reverse type enzymeimmunoassay (EIAs) with biotinylated antigens were used to determine IgM antibodies to measles virus in human sera. These antigens, either purified measles virus antigen or lysate type measles-vero antigen with lysate vero control antigen, were used in the two separate IgM-tests. Paired sera from 15 measles patients as well as 456 sera from patients with viral infections other than measles, with mycoplasma pneumoniae infections, from rheumatoid arthritis patients and blood donors, were assayed in a dilution of 1:200. Both the test systems detected all the 30 serum specimens from the measles patients as measles IgM positive, but the sera of all the other groups proved to be measles IgM negative. These tests developed for measles specific IgM antibodies, avoiding the interference of IgM-class rheumatoid factor, offer valuable tools for routine virus serology.
Tuokko
H
H
Department of Virology, University of Turku, Finland.
D000956:Antigens, Viral / Q000276:immunology*; D001710:Biotin; D007124:Immunoenzyme Techniques; D007075:Immunoglobulin M / Q000032:analysis*; D008457:Measles / Q000276:immunology; D008459:Measles virus / Q000276:immunology*
D000956:Antigens, Viral; D007075:Immunoglobulin M; D001710:Biotin