Purification and immunocytochemical detection of a protein that reveals layer V pyramidal cells in the rat cortex.
Protein 36 is a soluble protein isolated and purified from a high-speed supernatant fraction from homogenates of rat brain. To identify this protein in the brain, two-dimensional gel electrophoretograms of soluble proteins were prepared. The protein was separated by ion exchange and gel filtration chromatography. The chromatographic fractions were analyzed by two-dimensional electrophoresis. Protein 36 is a homodimer with a molecular weight of 64,000 Da and monomeric weight of 37,000 Da with an isoelectric point of 6.5. A rabbit antibody was raised to this protein. Immunocytochemical studies indicate that protein 36 is localized in large pyramidal cells, dendrites and axons of layer V of the cerebral cortex. The hippocampus contained cells in the stratum radiata and processes in the stratum pyramidalis. A variety of cell types were also observed in the globus pallidus, thalamus and hypothalamus.
Fukuda
T
T
Laboratory of Clinical Science, National Institute of Mental Health, Bethesda, MD 20892.
Jacobowitz
D M
DM
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002540:Cerebral Cortex / Q000032:analysis* / Q000166:cytology; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D007124:Immunoenzyme Techniques; D008297:Male; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification; D011712:Pyramidal Tracts / Q000032:analysis* / Q000166:cytology; D051381:Rats; D011919:Rats, Inbred Strains
D000596:Amino Acids; D009419:Nerve Tissue Proteins