Inaccurate protein synthesis in a mutant of Salmonella typhimurium defective in transfer RNA pseudouridylation.
Protein synthesis was studied comparatively in a wild-type strain of Salmonella typhimurium and in hisT mutant cells defective in the pseudouridylation of transfer RNA. From a quantitative point of view, no significant differences between the two types of strain was observed when measuring the rate of protein synthesis during either exponential growth or starvation for histidine. In contrast, the qualitative analysis of proteins by two-dimensional gel electrophoresis showed that histidine-starved hisT cells mistranslate the genetic program at a higher frequency than exponentially growing hisT cells or either starved or unstarved hisT+ cells.
Negre
D
D
Laboratoire de Biologie Moléculaire, Université de Lyon, Villeurbanne, France.
Cortay
J C
JC
Donini
P
P
Cozzone
A J
AJ
D001426:Bacterial Proteins / Q000096:biosynthesis*; D003062:Codon; D004591:Electrophoresis, Polyacrylamide Gel; D006639:Histidine / Q000235:genetics; D006836:Hydro-Lyases / Q000235:genetics*; D007525:Isoelectric Focusing; D009154:Mutation; D014176:Protein Biosynthesis; D011560:Pseudouridine / Q000378:metabolism*; D012345:RNA, Transfer, Amino Acid-Specific / Q000235:genetics*; D012354:RNA, Transfer, His / Q000235:genetics*; D012486:Salmonella typhimurium / Q000235:genetics* / Q000378:metabolism; D014529:Uridine / Q000031:analogs & derivatives*
D001426:Bacterial Proteins; D003062:Codon; D012345:RNA, Transfer, Amino Acid-Specific; D012354:RNA, Transfer, His; D011560:Pseudouridine; D006639:Histidine; D006836:Hydro-Lyases; C022479:pseudouridylate synthetase; D014529:Uridine