Intestinal mucin antigens in ulcerative colitis and their relationship with malignancy.
The expression of two intestinal mucin-associated antigens large intestine mucin antigen (LIMA) and small intestine mucin antigen (SIMA) were investigated by indirect immunoperoxidase staining of rectal mucosa from patients suffering from ulcerative colitis with (n = 6) and without (n = 31) associated carcinoma and in noncolitic controls (n = 40). The aim was to assess the relationship between antigen patterns and malignant change. SIMA, which is localised predominantly in the small intestine, is virtually undetectable in the normal adult colonic mucosa. However, this antigen is present in the foetal colon and colonic carcinoma. LIMA is expressed in normal colonic mucosa, but absent from the small intestine. LIMA staining patterns were not significantly different among the three groups. In contrast, expression of SIMA was significantly higher in the patients who had developed carcinoma (6/6) than in the noncancer group (7/71) (P less than 0.001). The presence of SIMA was also significantly related to areas of dysplasia compared to normal (P = .03) or inflammation (P less than .05), but it did not differ from mucosa showing "indefinite" atypia. The finding of 31% SIMA-positive biopsies associated with severe inflammation in colitis with active disease, but no evidence of malignancy, is difficult to explain at the present stage. A followup study would be necessary to determine its significance. Perhaps the most important finding is the increased frequency of SIMA-positive foci in histologically normal mucosa in carcinoma patients compared with the noncancer group (P less than .001), suggesting a field change. These observations may be prove useful for the identification of patients who may be at risk of developing carcinoma.
Filipe
M I
MI
Department of Histopathology, United Medical School of Guy's Hospital, London Bridge, England.
Sandey
A
A
Ma
J
J
D000328:Adult; D000368:Aged; D000951:Antigens, Neoplasm / Q000032:analysis*; D003093:Colitis, Ulcerative / Q000150:complications / Q000276:immunology* / Q000473:pathology; D003110:Colonic Neoplasms / Q000032:analysis* / Q000150:complications / Q000473:pathology; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D007413:Intestinal Mucosa / Q000276:immunology / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009077:Mucins; D011230:Precancerous Conditions / Q000032:analysis / Q000473:pathology; D012007:Rectum / Q000276:immunology / Q000473:pathology
D000951:Antigens, Neoplasm; D009077:Mucins; C042384:gastro-intestinal mucus-associated antigens