title: The core region of human glutaminyl-tRNA synthetase homologies with the Escherichia coli and yeast enzymes.
abstract: We have isolated from a Lambda-gt 11 library a human cDNA clone with one open reading frame of about 2400 bases. A stretch of about 350 amino acids in the deduced amino acid sequence is up to 40 percent identical with parts of the known amino acid sequences of E. coli and yeast glutaminyl (Gln)-tRNA synthetase. The isolated cDNA sequence corresponds to an internal section of a 5500 bases long mRNA that codes for a 170 kDa polypeptide associated with Gln-tRNA synthetase. Thus, the human enzyme is about three times larger than the E. coli and two times larger than the yeast Gln-tRNA synthetase. The three enzymes share an evolutionarily conserved core but differ in amino acid sequences linked to the N-terminal and C-terminal side of the core.
authors.affiliation: Fakultät für Biologie, Universität Konstanz, FRG.
authors.forename: P
authors.initials: P
authors.lastname: Thömmes
authors.forename: R
authors.initials: R
authors.lastname: Fett
authors.forename: B
authors.initials: B
authors.lastname: Schray
authors.forename: N
authors.initials: N
authors.lastname: Kunze
authors.forename: R
authors.initials: R
authors.lastname: Knippers
mesh_terms: D000595:Amino Acid Sequence; D000604:Amino Acyl-tRNA Synthetases / Q000235:genetics*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005975:Glutamate-tRNA Ligase / Q000235:genetics*; D008969:Molecular Sequence Data; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
chemical_list: D000604:Amino Acyl-tRNA Synthetases; D005975:Glutamate-tRNA Ligase