title: In vivo induction of malignant transformation of nude mouse B lymphocytes by a human multiple myeloma.
abstract: The bone marrow puncture fluid of human multiple myeloma was inoculated subcutaneously in BALB/C/nu nude mice. After passage of the xenograft from nude mice in vitro, a murine malignant lymphosarcoma cell line was unexpectedly established, which was named NLB-SK. The murine NLB-SK cell line has been cultivated in vitro over 72 passages for 10 months. Repeated cryopreservation showed that the murine NLB-SK cell line revived satisfactorily. Based on cell biological characteristics the malignant transformation might be attributed to horizontal oncogenesis between human malignant tumor cells and nude mouse normal somatic cells.
authors.affiliation: Central Laboratory of Electron Microscopy, Shandong Medical University, Jinan.
authors.forename: T X
authors.initials: TX
authors.lastname: Gao
authors.forename: L H
authors.initials: LH
authors.lastname: Zhou
authors.forename: Y B
authors.initials: YB
authors.lastname: Wang
authors.forename: W G
authors.initials: WG
authors.lastname: Zhang
authors.forename: Y H
authors.initials: YH
authors.lastname: Chen
authors.forename: J F
authors.initials: JF
authors.lastname: Liu
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000473:pathology*; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D005260:Female; D006801:Humans; D008228:Lymphoma, Non-Hodgkin / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008875:Middle Aged; D009101:Multiple Myeloma / Q000473:pathology*; D009368:Neoplasm Transplantation