Evidence for an accessory role of LFA-1 in lymphocyte-high endothelium interaction during homing.
In a variety of lymphocyte interactions, lymphocyte function-associated antigen-1 (LFA-1) plays an important role as an accessory mechanism mediating cell adhesion. We tested the possibility that LFA-1 could also be involved in the specific binding of lymphocytes to high endothelial venules (HEV) during homing. Antibodies against LFA-1 but not against various other cell surface molecules (except the putative gp90 homing receptor defined by the MEL-14 antibody) were found to inhibit in vitro adherence of lymphocytes to HEV in frozen sections of lymph nodes. Binding of T cell lines to HEV was also inhibited by anti-LFA-1 antibody. Using sublines selected for differential expression of the MEL-14 antigen, MEL-14 high cells (which bind well to HEV) were less susceptible to inhibition by anti-LFA-1 than poor binders with low levels of the homing receptor, supporting the model of LFA-1 being an accessory mechanism strengthening weak interactions between cells. Parallel results were found in vivo where anti-LFA-1 antibodies reduced the migration of normal lymphocytes into lymph nodes and Peyer's patches by 40 to 60%. Localization in the lung, especially of activated lymphocytes, was also impaired, although to a lesser extent. These findings suggest that LFA-1 plays an accessory role in cellular interactions relevant for lymphocyte migration.
Hamann
A
A
Department of Immunology, Universitätskrankenhaus Eppendorf, Hamburg, FRG.
Jablonski-Westrich
D
D
Duijvestijn
A
A
Butcher
E C
EC
Baisch
H
H
Harder
R
R
Thiele
H G
HG
D000818:Animals; D000911:Antibodies, Monoclonal / Q000502:physiology; D000954:Antigens, Surface / Q000276:immunology* / Q000502:physiology; D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D002450:Cell Communication; D002465:Cell Movement; D004727:Endothelium / Q000276:immunology*; D004729:Endothelium, Lymphatic / Q000166:cytology / Q000276:immunology* / Q000502:physiology; D005260:Female; D016169:Lymphocyte Function-Associated Antigen-1; D008214:Lymphocytes / Q000276:immunology* / Q000502:physiology; D051379:Mice; D008808:Mice, Inbred CBA; D013154:Spleen / Q000166:cytology
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D016169:Lymphocyte Function-Associated Antigen-1