Rat corticotropin-releasing hormone gene: sequence and tissue-specific expression.
The rat corticotropin releasing hormone (CRH) gene has been isolated and characterized by DNA sequence analysis. The gene exhibits a structural organization similar to that of the human CRH gene. The nucleotide sequence encoding the entire rat CRH precursor is located on the second exon, while exon I encodes the 5'-untranslated region of the mRNA. Analysis of the nucleotide sequence homology between the human and rat CRH genes reveals several highly conserved regions including the CRH peptide-encoding sequence and the 5'-flanking sequence. RNA blot analysis demonstrates that CRH mRNA can be observed in numerous regions of the rat brain as well as the spinal cord, adrenal gland, pituitary, and testis.
Thompson
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Vollum Institute for Advanced Biomedical Research, Oregon Health Sciences University, Portland 97201.
Seasholtz
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Herbert
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D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003346:Corticotropin-Releasing Hormone / Q000235:genetics*; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D015870:Gene Expression; D005796:Genes; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D009928:Organ Specificity; D051381:Rats; D012689:Sequence Homology, Nucleic Acid
D004247:DNA; D003346:Corticotropin-Releasing Hormone