Involvement of serum factor(s) adsorbed to the dish in the response of cycloheximide-pretreated BP-A31 cells to serum pulses.
The mitogenic effect of serum pulses, observed previously in quiescent BP-A31 cells, is an artifact due to adsorption of (unknown) serum mitogens to the culture dish; the continuous presence of growth factors is necessary for these cells to traverse the G1 phase. When pretreated with cycloheximide (CH) during the last 8-24 h of quiescence, the BP-A31 cells are particularly sensitive to the adsorbed serum mitogens, as well as to low concentrations of fetal calf serum (less than 0.25%) and to basic fibroblast growth factor. In contrast, the mitogenic activity of insulin was not influenced by CH pretreatment. The expression of the "competence genes" c-myc and JE was only transiently elevated in quiescent BP-A31 cells during CH exposure and did not correlate with the enhancement of the cells' sensitivity to mitogens.
Buchou
T
T
INSERM Unité 55, Hôpital Saint-Antoine, Paris, France.
Charollais
R H
RH
Mester
J
J
D000327:Adsorption; D000818:Animals; D001769:Blood; D002453:Cell Cycle / Q000187:drug effects; D002460:Cell Line; D003470:Culture Media; D003513:Cycloheximide / Q000494:pharmacology*; D005346:Fibroblast Growth Factors / Q000494:pharmacology; D005347:Fibroblasts; D005796:Genes / Q000187:drug effects; D006133:Growth Substances / Q000097:blood* / Q000378:metabolism / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology; D007399:Interphase / Q000187:drug effects; D008934:Mitogens / Q000097:blood* / Q000378:metabolism / Q000494:pharmacology; D008938:Mitosis / Q000187:drug effects*; D009857:Oncogenes; D012333:RNA, Messenger / Q000096:biosynthesis; D013498:Suramin / Q000494:pharmacology
D003470:Culture Media; D006133:Growth Substances; D007328:Insulin; D008934:Mitogens; D012333:RNA, Messenger; D013498:Suramin; D005346:Fibroblast Growth Factors; D003513:Cycloheximide