H-2-dependent binding of xenogeneic beta 2-microglobulin from culture media.
Sera of C57BL/6 mice contained lymphocytotoxic antibodies after injections with syngeneic lymphoblasts. The antibodies were directed against bovine beta 2-microglobulin, a component of the culture medium, and mimicked H-2-specific antibodies by a preferential recognition of target cells expressing certain H-2 Ag. This "polymorphic" reactivity pattern was due to a variable capacity of H-2 molecules associated with bovine beta 2-microglobulin.
Kievits
F
F
Central Laboratory, Netherlands Red Cross Blood Transfusion Service, Amsterdam.
Boerenkamp
W J
WJ
Ivanyi
P
P
D000818:Animals; D000961:Antilymphocyte Serum / Q000502:physiology; D001666:Binding Sites, Antibody; D002417:Cattle; D003470:Culture Media / Q000032:analysis*; D005312:Fetal Blood / Q000276:immunology; D006183:H-2 Antigens / Q000235:genetics / Q000276:immunology*; D008213:Lymphocyte Activation; D017710:Lymphocyte Transfusion; D051379:Mice; D008810:Mice, Inbred C57BL; D013154:Spleen / Q000166:cytology; D001613:beta 2-Microglobulin / Q000235:genetics / Q000276:immunology*
D000961:Antilymphocyte Serum; D003470:Culture Media; D006183:H-2 Antigens; D001613:beta 2-Microglobulin