A comparative immunofluorescent study of fixed decalcified tissue and frozen non-decalcified tissue from the guinea pig cochlea.
The immunofluorescence test (IF) is an important diagnostic laboratory tool for detecting immune-mediated sensorineural hearing loss (SNHL). The results of applying the IF to ethanol-fixed, paraffin-embedded, decalcified sections of guinea pig cochlea as compared with frozen non-decalcified preparations are presented. Sera from ten patients with SNHL were tested in addition to antibody-positive and negative human serum controls. Despite fixation and prolonged decalcification, deparaffinized cochlear sections showed a strong positive reaction with most of the positive serum controls. Anti-endothelial antibodies were detected in the serum of one patient and anti-nuclear antibodies in another. The fine cochlear structure was clearly delineated. However, the decalcified specimens failed to react with one serum model, which revealed a positive fluorescence when incubated with the frozen non-decalcified cochlear preparations. These latter findings denote possible leaching of the antigenic material from the sections. This study further shows that frozen nondecalcified cochlear tissue is a reliable tissue source for the interpretation of the results obtained with IF.
Soliman
A M
AM
Department of Otolaryngology, University of Tübingen, Federal Republic of Germany.
D000818:Animals; D003051:Cochlea / Q000033:anatomy & histology*; D003648:Decalcification Technique; D005404:Fixatives; D005455:Fluorescent Antibody Technique; D005629:Frozen Sections; D006168:Guinea Pigs
D005404:Fixatives