Excretion of cholera toxin from Escherichia coli: a potential oral vaccine for cholera.
Escherichia coli strain N100 has been mutagenized by transposon mutagenesis and mutants with a cell surface leaky phenotype have been isolated. The mutant designated as E. coli N100::Tn5 excreted periplasmic proteins like ribonuclease and alkaline phosphatase. When this mutant strain was transformed with plasmids containing cloned cholera toxin genes, the toxin protein synthesized in the cells were excreted. The potentiality of this strain as a live oral vaccine for cholera has been discussed.
Dasgupta
U
U
Biophysics Division, Indian Institute of Chemical Biology, Calcutta.
Guhathakurta
I
I
Das
J
J
D000284:Administration, Oral; D000469:Alkaline Phosphatase / Q000032:analysis; D001681:Biological Assay; D002772:Cholera Toxin / Q000378:metabolism*; D022121:Cholera Vaccines / Q000008:administration & dosage; D004926:Escherichia coli / Q000378:metabolism*; D007082:Ileum / Q000187:drug effects; D010641:Phenotype
D022121:Cholera Vaccines; D002772:Cholera Toxin; D000469:Alkaline Phosphatase