title: Monoclonal antibodies induce the translocation, patching, and shedding of surface antigens of sea urchin spermatozoa.
abstract: Monoclonal antibodies reacting with external domains of plasma membrane proteins of sea urchin spermatozoa cause the reversible aggregation of sperm. The rate of disaggregation is temperature dependent indicating the involvement of membrane fluidity. Immunofluorescence shows that disaggregation is temporally correlated with the movement of surface-bound antibody into large aggregates which are then shed from the cell surface. Electrophoretic analysis of the shed antigens shows that they resemble the total complement of proteins of isolated sperm membranes. The translocation and shedding of surface-bound ligands may be a common property of eukaryotic flagellar membranes.
authors.affiliation: Marine Biology Research Division, Scripps Institution of Oceanography, University of California, San Diego 92093.
authors.forename: J S
authors.initials: JS
authors.lastname: Trimmer
authors.forename: V D
authors.initials: VD
authors.lastname: Vacquier
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000937:Antigen-Antibody Reactions; D000954:Antigens, Surface / Q000276:immunology*; D002449:Cell Aggregation; D002462:Cell Membrane / Q000502:physiology; D005455:Fluorescent Antibody Technique; D008297:Male; D008562:Membrane Glycoproteins / Q000502:physiology*; D010063:Ovum / Q000502:physiology; D012617:Sea Urchins; D013094:Spermatozoa / Q000276:immunology*; D013696:Temperature
chemical_list: D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D008562:Membrane Glycoproteins