title: The effects of human interferon-alpha upon in vitro canine immune responses.
abstract: The effects of varying amounts (100.0-0.01 units/ml) of human interferon (IFN) alpha upon in vitro canine immune responses were studied. Exogenous heterologous species IFN-alpha suppressed B-cell differentiation in a dose-dependent fashion and enhanced interleukin-2 production (P less than 0.05) by activated T-lymphocytes. Interferon enhanced natural killer (NK) cytotoxicity when tested against NK-resistant target cells (less than 0.05). One hundred units IFN/ml increased interleukin-1 production by canine monocytes, but this effect was not statistically significant. Exogenous IFN had no discernible effect upon lectin-induced lymphocyte blastogenesis. The results of this study demonstrate that human IFN-alpha does affect various canine lymphocyte functions and these effects depend upon the in vitro assay system employed.
authors.affiliation: Department of Veterinary Pathobiology, College of Veterinary Medicine, Ohio State University, Columbus.
authors.forename: S
authors.initials: S
authors.lastname: Krakowka
authors.forename: J M
authors.initials: JM
authors.lastname: Cummins
authors.forename: S S
authors.initials: SS
authors.lastname: Ringler
mesh_terms: D000276:Adjuvants, Immunologic; D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D002454:Cell Differentiation; D004285:Dogs / Q000276:immunology*; D006801:Humans; D007109:Immunity; D066298:In Vitro Techniques; D007370:Interferon Type I / Q000494:pharmacology*; D007375:Interleukin-1 / Q000096:biosynthesis; D007376:Interleukin-2 / Q000096:biosynthesis; D007694:Killer Cells, Natural / Q000276:immunology; D008213:Lymphocyte Activation; D013045:Species Specificity
chemical_list: D000276:Adjuvants, Immunologic; D007370:Interferon Type I; D007375:Interleukin-1; D007376:Interleukin-2