Stimulation of aromatase activity in immature porcine Leydig cells by fibroblast growth factor (FGF).
The effects of fibroblast growth factor (FGF) on testicular aromatase activity has been studied using primary cultures of porcine Leydig cells. After culture for 3 days in the absence or presence of FGF, the ability of the cells to produce estrogen was examined in a 4h-test period in which either (a) hCG (10(-9) M) or (b) androstenedione (3 x 10(-6) M) was added to the medium. FGF produced a 3- to 20-fold increase in estrogen formation from endogenous or exogenous substrate during the test period, in spite of a marked decrease (approximately equal to 60%) in [125I]-hCG binding and no significant change in testosterone concentration. Stimulation of estrogen secretion by FGF was dose-(ED50 approximately equal to 2 ng/ml) and time-dependent, the first and maximal effects were observed after 12h and 48h, respectively. Preliminary tests with several other factors (insulin, EGF, TGF-beta, FSH and hCG) showed that hCG alone directly stimulated aromatase activity. From these findings a role is suggested for FGF as a paracrine/autocrine agent in the control of estrogen secretion by Leydig cells.
Raeside
J I
JI
INSERM U307, Hopital Debrousse, Lyon, France.
Berthelon
M C
MC
Sanchez
P
P
Saez
J M
JM
D000818:Animals; D001141:Aromatase / Q000378:metabolism*; D002478:Cells, Cultured; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004967:Estrogens / Q000096:biosynthesis*; D005346:Fibroblast Growth Factors / Q000494:pharmacology*; D007328:Insulin / Q000494:pharmacology; D007985:Leydig Cells / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D013552:Swine; D013739:Testosterone / Q000096:biosynthesis
D006063:Chorionic Gonadotropin; D004967:Estrogens; D007328:Insulin; D013739:Testosterone; D005346:Fibroblast Growth Factors; D001141:Aromatase