DNA flow cytometric values in bladder carcinoma biopsies obtained from fresh and paraffin-embedded material.
DNA-histograms were obtained by flow cytometry (FCM) of nuclei prepared from fresh bladder carcinoma biopsies and from 100 micron sections of paraffin-embedded material from the same biopsies. Linear regression analysis of ploidy values from fresh material versus those from paraffin blocks showed excellent correlation (R2 = 0.957). Owing to the high background, the paraffin-embedded material was less suited for analysis of S-phase fraction. It is concluded that DNA FCM of nuclei obtained from paraffin-embedded bladder carcinoma biopsies yields reliable results concerning ploidy, but does not permit evaluation of the S-phase fraction.
Jacobsen
A B
AB
Department of Medical Oncology, Norweigian Radium Hospital, Oslo, Norway.
Fosså
S D
SD
Thorud
E
E
Lunde
S
S
Melvik
J E
JE
Pettersen
E O
EO
D001706:Biopsy; D004273:DNA, Neoplasm / Q000032:analysis*; D005434:Flow Cytometry; D006652:Histological Techniques; D006801:Humans; D010232:Paraffin; D001749:Urinary Bladder Neoplasms / Q000032:analysis*
D004273:DNA, Neoplasm; D010232:Paraffin