title: Isolation of alligator gar (Lepisosteus spatula) glucagon, oxyntomodulin, and glucagon-like peptide: amino acid sequences of oxyntomodulin and glucagon-like peptide.
abstract: Oxyntomodulin, glucagon, and a glucagon-like peptide (GLP) have been isolated from the endocrine pancreas of the alligator gar (Lepisosteus spatula), a ganoid fish. The three peptides were isolated by gel filtration and HPLC and were identified by size, composition, and glucagon-like immunoreactivity. The amino acid sequences of the oxyntomodulin and GLP were determined. The oxyntomodulin contains 36 amino acid residues and its sequence is H S Q G T F T N D Y S K Y L D T R R A Q D F V Q W L M S T K R S G G I T. The composition of the glucagon is identical to the N-terminal 29 residues of the gar oxyntomodulin. The single form of GLP found contains 34 amino acid residues in the following sequence: H A D G T Y T S D V S S Y L Q D Q A A K K F V T W L K Q G Q D R R E. These findings suggest that all three peptides are derived from a common precursor.
authors.affiliation: Department of Biochemistry, University of Kansas Medical Center, Kansas City 66103.
authors.forename: H G
authors.initials: HG
authors.lastname: Pollock
authors.forename: J R
authors.initials: JR
authors.lastname: Kimmel
authors.forename: K E
authors.initials: KE
authors.lastname: Ebner
authors.forename: J W
authors.initials: JW
authors.lastname: Hamilton
authors.forename: J B
authors.initials: JB
authors.lastname: Rouse
authors.forename: V
authors.initials: V
authors.lastname: Lance
authors.forename: A B
authors.initials: AB
authors.lastname: Rawitch
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D005260:Female; D005399:Fishes / Q000378:metabolism*; D005768:Gastrointestinal Hormones / Q000302:isolation & purification*; D005934:Glucagon / Q000302:isolation & purification*; D004763:Glucagon-Like Peptides / Q000302:isolation & purification*; D007515:Islets of Langerhans / Q000032:analysis*; D008297:Male; D008969:Molecular Sequence Data; D053772:Oxyntomodulin; D010455:Peptides / Q000302:isolation & purification*; D011863:Radioimmunoassay
chemical_list: D000596:Amino Acids; D005768:Gastrointestinal Hormones; D053772:Oxyntomodulin; D010455:Peptides; D004763:Glucagon-Like Peptides; C015236:glucagon-like-immunoreactivity; D005934:Glucagon