title: Frequency of deletional types of alpha-thalassemia in Kampuchea.
abstract: The frequency of deletional types of alpha-thalassemias in the Khmer population of Kampuchea (Cambodia) was estimated using DNA techniques. Among 58 healthy adult Kampucheans from rural areas, 17 had alpha-globin gene anomalies. There were 14 heterozygotes and two homozygotes for alpha(+)-thalassemia; the remaining test subject carried a deletion of both alpha-globin genes (alpha(0) -thalassemia of the Southeast Asian type) on one chromosome 16, and triple alpha-globin genes on the other. All of the 18 alpha(+)-thalassemia deletions were of the -alpha 3.7 type (17 subtype I, 1 subtype II). The restriction pattern obtained with the enzyme RsaI and comparison of the intensity of hybridization with alpha-globin and beta-globin gene probes yielded no evidence of total deletion of the alpha-gene complex. The prevalence of deletional alpha(+)-thalassemia in Kampuchea is higher, and that of alpha(0)-thalassemia is lower than in neighbouring Thailand.
authors.affiliation: Department of Human Genetics, Medizinische Hochschule Hannover, Federal Republic of Germany.
authors.forename: J
authors.initials: J
authors.lastname: Hundrieser
authors.forename: T
authors.initials: T
authors.lastname: Sanguansermsri
authors.forename: S D
authors.initials: SD
authors.lastname: Flatz
authors.forename: W
authors.initials: W
authors.lastname: Kühnau
authors.forename: M
authors.initials: M
authors.lastname: Pape
authors.forename: T
authors.initials: T
authors.lastname: Papp
authors.forename: G
authors.initials: G
authors.lastname: Flatz
mesh_terms: D015139:Blotting, Southern; D002160:Cambodia; D002872:Chromosome Deletion; D005787:Gene Frequency; D006239:Haplotypes; D006801:Humans; D015183:Restriction Mapping; D013789:Thalassemia / Q000453:epidemiology / Q000235:genetics*