Both N-ras and c-myc are activated in the SHAC human stomach fibrosarcoma cell line.
A transforming N-ras gene was isolated from the SHAC human stomach fibrosarcoma cell line. A single-point mutation resulting in the substitution of histidine for glutamine at codon 61 was found in the SHAC transforming allele. The N-ras gene is overexpressed in the tumor cells and transformant cells. The N-ras p21 product was studied by immunoprecipitation and showed no alteration in mobility as compared to the normal p21 protein. The c-myc gene is amplified and overexpressed in these cells. This report gives evidence that an amplified c-myc and a mutated N-ras gene are both present in this tumor cell line and provides support for the idea that co-operation of at least 2 activated cellular oncogenes is required for carcinogenesis.
Andeol
Y
Y
Groupe Pluridisciplinaire de Recherches sur le Cancer, ER 278 du CNRSA, Institut de Recherches Scientifiques sur le Cancer, Villejuif, France.
Nardeux
P C
PC
Daya-Grosjean
L
L
Brison
O
O
Cebrian
J
J
Suarez
H
H
D000818:Animals; D001483:Base Sequence; D002471:Cell Transformation, Neoplastic; D003001:Cloning, Molecular; D003062:Codon; D004274:DNA, Recombinant; D005091:Exons; D005354:Fibrosarcoma / Q000235:genetics*; D005784:Gene Amplification; D005786:Gene Expression Regulation; D006801:Humans; D008297:Male; D051379:Mice; D008875:Middle Aged; D009154:Mutation; D011518:Proto-Oncogene Proteins / Q000235:genetics; D016271:Proto-Oncogene Proteins c-myc; D016283:Proto-Oncogene Proteins p21(ras); D011519:Proto-Oncogenes; D012333:RNA, Messenger / Q000235:genetics; D013274:Stomach Neoplasms / Q000235:genetics*; D014162:Transfection; D014407:Tumor Cells, Cultured
D003062:Codon; D004274:DNA, Recombinant; D011518:Proto-Oncogene Proteins; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger; C501009:HRAS protein, human; D016283:Proto-Oncogene Proteins p21(ras)