Methyl-directed DNA mismatch repair in Escherichia coli.
Some of the molecular aspects of methyl-directed mismatch repair in E. coli have been characterized. These include: mismatch recognition by mutS protein in which different mispairs are bound with different affinities; the direct involvement of d(GATC) sites; and strand scission by mutH protein at d(GATC) sequences with strand selection based on methylation of the DNA at those sites. In addition, communication over a distance between a mismatch and d(GATC) sites has been implicated. Analysis of mismatch correction in a defined system (Lahue et al., unpublished) should provide a direct means to further molecular aspects of this process.
Lahue
R S
RS
Department of Biochemistry, Duke University Medical Center, Durham, NC 27710.
Modrich
P
P
D000225:Adenine / Q000031:analogs & derivatives* / Q000502:physiology; D001426:Bacterial Proteins / Q000502:physiology; D001482:Base Composition; D011088:DNA Ligases / Q000502:physiology; D004260:DNA Repair; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000502:physiology; D004706:Endodeoxyribonucleases / Q000502:physiology; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D008745:Methylation; D008780:Methyltransferases / Q000502:physiology; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific)
D000225:Adenine; D001426:Bacterial Proteins; D011088:DNA Ligases; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; D004706:Endodeoxyribonucleases; D008780:Methyltransferases; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific); C000730567:6-methyladenine