Calmodulin-binding profiles for nebulin and dystrophin in human skeletal muscle.
Nebulin and dystrophin are two high-molecular-mass skeletal muscle proteins that have both been associated with the defective gene in Duchenne muscular dystrophy, although the function of neither protein is known. Other high-molecular-mass, calmodulin-binding proteins have recently been implicated in regulating calcium release from skeletal muscle. Western blots of human skeletal muscle biopsy samples were probed with biotinylated calmodulin; nebulin was identified as a prominent high-molecular-mass calmodulin-binding protein but dystrophin did not bind detectable amounts of biotinylated calmodulin. Dystrophin was absent in a Duchenne muscle biopsy.
Patel
K
K
Jerry Lewis Muscle Research Centre, Department of Paediatrics, Royal Postgraduate Medical School, London, England.
Strong
P N
PN
Dubowitz
V
V
Dunn
M J
MJ
D002147:Calmodulin / Q000378:metabolism*; D016189:Dystrophin; D006801:Humans; D008970:Molecular Weight; D009124:Muscle Proteins / Q000378:metabolism*; D009132:Muscles / Q000378:metabolism*; D009136:Muscular Dystrophies / Q000378:metabolism; D011485:Protein Binding; D012016:Reference Values
D002147:Calmodulin; D016189:Dystrophin; D009124:Muscle Proteins; C035620:nebulin