title: Mutants of Aspergillus nidulans with increased resistance to the alkylating agent, N-methyl-N'-nitro-N-nitrosoguanidine.
abstract: The isolation and characterisation of mutants of Aspergillus nidulans showing resistance to MNNG is described. Such isolates were stable through prolonged subculture in the absence of the selective agent, and resistance segregated as an allele of a single gene in meiotic and mitotic analysis. MNNG-resistant strains showed an increase in resistance to EMS and UV irradiation but no cross-resistance to MMS was detected. Possible mechanisms of resistance to alkylating agents are discussed.
authors.affiliation: Department of Genetics, University of Liverpool, Great Britain.
authors.forename: P
authors.initials: P
authors.lastname: Hooley
authors.forename: S G
authors.initials: SG
authors.lastname: Shawcross
authors.forename: P
authors.initials: P
authors.lastname: Strike
mesh_terms: D001233:Aspergillus nidulans / Q000187:drug effects* / Q000235:genetics / Q000528:radiation effects; D004352:Drug Resistance, Microbial; D005020:Ethyl Methanesulfonate / Q000494:pharmacology; D005800:Genes, Fungal; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology*; D011836:Radiation Tolerance; D013172:Spores, Fungal; D014466:Ultraviolet Rays
chemical_list: D008769:Methylnitronitrosoguanidine; D005020:Ethyl Methanesulfonate