Clonal proliferation in vitro of individual murine and human hemopoietic cells after fluorescence-activated cell sorting.
A modification of the fluorescence-activated cell sorter (FACS) was used to rapidly and reliably study the clonal proliferation of single hemopoietic cells. Murine FDC-P1 and human cord blood progenitor cells were examined for their ability to proliferate from single cells in 96-well microtiter plates containing agar medium and appropriate stimuli. FACS-sorted FDC-P1 single cells formed colonies in 345 out of 558 wells (62%), which compared favorably with control cultures (53%) and micromanipulated single cells (55%). Similarly, the frequency and type of day-14 colonies arising from cord blood progenitor cells when sorted as single cells by the FACS compared favorably with those grown from micromanipulated single cells or in control cultures.
Kannourakis
G
G
Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia.
Johnson
G R
GR
Battye
F
F
D000818:Animals; D002455:Cell Division; D002460:Cell Line; D002469:Cell Separation / Q000379:methods*; D003115:Colony-Stimulating Factors / Q000494:pharmacology; D005312:Fetal Blood; D005453:Fluorescence; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D006133:Growth Substances / Q000494:pharmacology; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D051379:Mice
D003115:Colony-Stimulating Factors; D006133:Growth Substances; D016178:Granulocyte-Macrophage Colony-Stimulating Factor