Sufficiency of the Klenow fragment for survival of polC(Ts) pcbA1 Escherichia coli at 43 degrees C.
Escherichia coli cells with the pcbA1 allele and temperature-sensitive DNA polymerase III mutations survived at a restrictive temperature if DNA polymerase I activity was present. The Klenow fragment of DNA polymerase I was capable of supporting cell survival as well.
Bryan
S K
SK
Department of Cell Biology, Baylor College of Medicine, Houston, Texas 77030.
Moses
R E
RE
D004256:DNA Polymerase I / Q000378:metabolism*; D004258:DNA Polymerase III / Q000235:genetics; D004261:DNA Replication; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D009154:Mutation; D010957:Plasmids; D013696:Temperature; D014169:Transformation, Bacterial
D004256:DNA Polymerase I; D004258:DNA Polymerase III