Routine and rapid enzyme linked immunosorbent assays for circulating anti-glomerular basement membrane antibodies.
Microtitre plate modifications of the original tube enzyme linked immunosorbent assay (ELISA) of Wheeler and Sussman were used for the routine and rapid assays of anti-glomerular basement membrane antibodies in human sera. In a prospective study of 238 sera from 200 patients, the routine assay (about 24 hours) detected circulating antibodies in seven sera from three patients with active anti-glomerular basement membrane disease. The remaining sera, from patients with a variety of other glomerulonephropathies, were negative by the assay. The rapid assays took less than four hours, and in a retrospective study, detected anti-glomerular basement membrane antibodies in a range of 15 positive sera, with a level of discrimination similar to that observed in the routine assay and with no false positive/negative results.
Wheeler
J
J
Departments of Histopathology, University of Newcastle upon Tyne.
Simpson
J
J
Morley
A R
AR
D000906:Antibodies / Q000032:analysis*; D001485:Basement Membrane / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005188:False Negative Reactions; D005189:False Positive Reactions; D006801:Humans; D007678:Kidney Glomerulus / Q000276:immunology*; D008722:Methods; D012680:Sensitivity and Specificity; D013997:Time Factors
D000906:Antibodies