Cloning and expression of secreted antigens of Clostridium difficile in Escherichia coli.
The feasibility of the cloning and expression of Clostridium difficile antigens in Escherichia coli was investigated. The expression of a limited number of cloned clostridial antigens under the control of clostridial promoter elements in E. coli was observed.
Dailey
D C
DC
Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis 46223.
Schloemer
R H
RH
D000942:Antigens, Bacterial / Q000235:genetics* / Q000302:isolation & purification; D003001:Cloning, Molecular; D003013:Clostridium / Q000235:genetics / Q000276:immunology*; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D014162:Transfection
D000942:Antigens, Bacterial