title: Ion microscopy: a new approach for subcellular localization of labelled molecules.
abstract: Secondary ion mass spectroscopy (SIMS) was used to obtain images representing the intracellular distribution of molecules labelled with carbon 14. Deoxyadenosine labelled with carbon 14 was added to a cultured human fibroblast cell medium, and the intracellular distribution of this molecule was studied using three different SIMS instruments: the CAMECA IMS 3F and SMI 300 ion microscopes and the UC-HRL scanning ion microprobe. Carbon 14 distribution images obtained by this method show that deoxyadenosine U-C14 is present in the cytoplasm as well as the nucleus, with a higher concentration in the nucleoli. Our study clearly demonstrates that ion microscopy is well suited for carbon 14 detection and localization at the subcellular level, permitting a wide variety of microanalytical tracer experiments.
authors.affiliation: Laboratoire de Biophysique, SC 27 de l'INSERM, Faculté de Médecine, Créteil France.
authors.forename: E
authors.initials: E
authors.lastname: Hindie
authors.forename: P
authors.initials: P
authors.lastname: Hallégot
authors.forename: J M
authors.initials: JM
authors.lastname: Chabala
authors.forename: N A
authors.initials: NA
authors.lastname: Thorne
authors.forename: B
authors.initials: B
authors.lastname: Coulomb
authors.forename: R
authors.initials: R
authors.lastname: Levi-Setti
authors.forename: P
authors.initials: P
authors.lastname: Galle
mesh_terms: D000328:Adult; D002250:Carbon Radioisotopes; D002478:Cells, Cultured; D003839:Deoxyadenosines / Q000032:analysis* / Q000378:metabolism; D005260:Female; D005347:Fibroblasts / Q000032:analysis* / Q000378:metabolism / Q000648:ultrastructure; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D008855:Microscopy, Electron, Scanning / Q000379:methods*; D011877:Radionuclide Imaging / Q000379:methods
chemical_list: D002250:Carbon Radioisotopes; D003839:Deoxyadenosines