Partial chimerism after T-cell-depleted allogeneic bone marrow transplantation in leukemic HLA-matched patients: a cytogenetic documentation.
We evaluated serially by cytogenetics the blood and marrow chimerism of 38 leukemic recipients of HLA-matched bone marrow transplants (BMT) who were prepared by high doses of alkylating agents and fractionated total-body irradiation (2.2 Gy X 5). Donor or host mitoses were identified by examination of sex chromosomes in 32 patients or by evaluation of the polymorphism of other chromosomes after specific banding in six patients. Twenty-four patients were recipients of untreated BMT, and 14 were recipients of T-cell-depleted BMT. In the 24 patients who received untreated BMT, all showed successful engraftment, and only three had a transient mixed chimera. In the 14 recipients of T-cell-depleted BMTs, four rejected their grafts, and seven had mixed chimeras; these mixed chimeras were more frequent in blood lymphocytes than in marrow cells and could be detected up to 26 months after BMT. This high frequency of partial chimerism after T-cell-depleted BMT by comparison with a control group suggests that the donor's T cells play an important role in the eradication of host residual hematopoiesis after allogeneic BMT.
Bertheas
M F
MF
Cytogenetic Laboratory of the Blood Bank, Saint Etienne, France.
Maraninchi
D
D
Lafage
M
M
Fraisse
J
J
Blaise
D
D
Stoppa
A M
AM
Michel
G
G
Brizard
C P
CP
Gaspard
M H
MH
Novakovitch
G
G
D000293:Adolescent; D000328:Adult; D001853:Bone Marrow / Q000473:pathology; D016026:Bone Marrow Transplantation; D002648:Child; D002675:Child, Preschool; D002678:Chimera; D002871:Chromosome Banding; D003582:Cytogenetics; D005500:Follow-Up Studies; D006086:Graft vs Host Disease / Q000209:etiology / Q000235:genetics / Q000517:prevention & control; D006680:HLA Antigens / Q000235:genetics; D006650:Histocompatibility Testing; D006801:Humans; D007938:Leukemia / Q000097:blood / Q000235:genetics* / Q000473:pathology; D008212:Lymphocyte Depletion; D013601:T-Lymphocytes; D014184:Transplantation, Homologous / Q000009:adverse effects / Q000379:methods
D006680:HLA Antigens