title: The presence and origin of phosphopeptides in human saliva.
abstract: The presence of phosphopeptides in whole saliva (saliva expectorated from the mouth) was demonstrated and their origin was evaluated. Whole saliva contained much larger numbers of small phosphopeptides than are found in the glandular secretions. Most of these originated from the acidic proline-rich proteins (PRPs) in the major salivary glands and were formed, after secretion into the oral cavity, as a result of rapid degradation by proteolytic enzymes from extraglandular sources contained in sediment from whole saliva. Some peptides may have been formed by cleavage of basic PRPs, but other phosphoproteins apparently contributed little to the observed phosphopeptides. Most of the enzymes that produced phosphopeptides are serine proteinases. The gel-electrophoretic band patterns of the phosphopeptides obtained from 26 individuals of various acidic-PRP phenotypes were remarkably similar, demonstrating that the enzymes responsible were generally present in the population surveyed and that similar cleavages occur regardless of the nature of the acidic PRPs. Many of these peptides were N-terminal proteolytic cleavage products of acidic PRPs. The N-terminal phosphorylated region of acidic PRPs contains various biological activities, such as inhibition of hydroxyapatite formation, calcium binding and binding to hydroxyapatite, the major mineral of teeth. The demonstration of these phosphopeptides in the saliva that is in contact with the oral surface may therefore be of biological importance.
authors.affiliation: Department of Biochemistry, University of Toronto, Ont., Canada.
authors.forename: K
authors.initials: K
authors.lastname: Minaguchi
authors.forename: G
authors.initials: G
authors.lastname: Madapallimattam
authors.forename: A
authors.initials: A
authors.lastname: Bennick
mesh_terms: D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007163:Immunosorbent Techniques; D010641:Phenotype; D010664:Phenylmethylsulfonyl Fluoride; D010748:Phosphopeptides / Q000235:genetics / Q000378:metabolism*; D012463:Saliva / Q000378:metabolism*
chemical_list: D010748:Phosphopeptides; D010664:Phenylmethylsulfonyl Fluoride