Response of resident murine peritoneal macrophages to in vivo administration of granulocyte-macrophage colony-stimulating factor.
The effect of s.c. inoculation of purified recombinant derived granulocyte-macrophage (GM)-CSF on resident murine peritoneal macrophages was assessed in this study. From 18 to 24 h after s.c. administration of GM-CSF to normal mice, the resident peritoneal macrophages were harvested and the levels of membrane-bound IL-1, FcR, Mac-1 cell-surface Ag, and class II MHC expression were assessed. Peritoneal cells from GM-CSF-inoculated mice had significantly greater levels of membrane-bound IL-1 than did control mice. In addition when resident peritoneal macrophages from normal mice were purified by adherence and grown in the presence of GM-CSF, they produced greater levels of both membrane-bound and secreted IL-1. The peritoneal cells from GM-CSF-inoculated mice did not differ from controls in the expression of class II MHC-encoded Ag. This observation was confirmed by the finding that GM-CSF was unable to induce class II MHC expression on P388D1 cells, whereas a secondary mixed leukocyte culture supernatant was. Peritoneal cells from GM-CSF-inoculated mice also exhibited greater levels of expression of FcR and the Mac-1 cell-surface Ag. This resulted in an increase in their ability to phagocytose opsonized SRBC in vitro.
Morrissey
P J
PJ
Immunex Corporation, Seattle, WA 98101.
Bressler
L
L
Charrier
K
K
Alpert
A
A
D000818:Animals; D000954:Antigens, Surface / Q000096:biosynthesis; D003115:Colony-Stimulating Factors / Q000494:pharmacology*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D007375:Interleukin-1 / Q000096:biosynthesis; D016177:Macrophage-1 Antigen; D008264:Macrophages / Q000032:analysis / Q000187:drug effects*; D051379:Mice; D008810:Mice, Inbred C57BL; D010529:Peritoneal Cavity; D011961:Receptors, Fc / Q000096:biosynthesis; D011994:Recombinant Proteins / Q000494:pharmacology; D013268:Stimulation, Chemical
D000954:Antigens, Surface; D003115:Colony-Stimulating Factors; D000949:Histocompatibility Antigens Class II; D007375:Interleukin-1; D016177:Macrophage-1 Antigen; D011961:Receptors, Fc; D011994:Recombinant Proteins