Characterization of an hospital disseminated plasmid encoding resistance to gentamicin and other antimicrobial agents.
A preliminary report has shown the existence of an endemic R plasmid in the University Hospital of Zaragoza. The results presented in this paper demonstrate the dissemination of a new 73 kilobases plasmid into multiple strains and species of gram-negative bacilli. This transferable plasmid belongs to Incompatibility group P and mediates resistance to ampicillin, tetracycline, gentamicin, kanamycin, streptomycin, chloramphenicol and sulfamethoxazole, synthesizing the aminoglycoside-modifying enzymes 3-acetyltransferase, 3'phosphotransferase, and 3"nucleotidyltransferase, and a TEM-1 beta-lactamase. These results and the previous findings show that a family of gentamicin-resistance plasmids exists among the gram-negative bacteria in the University Hospital. Resistance to gentamicin in all these plasmids is associated with the formation of 3-N-acetyltransferases.
Rivera
M J
MJ
Department of Microbiology, Faculty of Medicine, University of Zaragoza, Spain.
Martín
C
C
Gómez-Lus
M L
ML
Robledano
L
L
Otal
I
I
Gómez-Lus
R
R
D001424:Bacterial Infections / Q000382:microbiology*; D001426:Bacterial Proteins / Q000032:analysis; D003428:Cross Infection / Q000382:microbiology*; D004269:DNA, Bacterial / Q000032:analysis; D004352:Drug Resistance, Microbial; D005839:Gentamicins / Q000494:pharmacology; D006090:Gram-Negative Bacteria / Q000187:drug effects* / Q000201:enzymology / Q000302:isolation & purification; D006801:Humans; D011815:R Factors
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D005839:Gentamicins