title: Polyamine metabolism and interconversion in NIH 3T3 and ras-transfected NIH 3T3 cells.
abstract: The effect of transfection of NIH 3T3 cells by the human ras (c-Ha-ras-1) oncogene on uptake, interconversion, and excretion of polyamines was studied. Uptake and interconversion of spermidine were higher in the ras-transfected cells. Acetylpolyamines were excreted into the medium by the ras-transfected cells, whereas they were retained by NIH 3T3 cells. In addition to acetylpolyamines, some unknown polyamine conjugates occurred in the ras-transfected cells.
authors.affiliation: Department of Molecular Biology, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
authors.forename: R
authors.initials: R
authors.lastname: Pakala
authors.forename: M
authors.initials: M
authors.lastname: Kreisel
authors.forename: U
authors.initials: U
authors.lastname: Bachrach
mesh_terms: D000107:Acetylation; D002471:Cell Transformation, Neoplastic / Q000378:metabolism*; D002478:Cells, Cultured; D011905:Genes, ras; D011073:Polyamines / Q000378:metabolism*; D014162:Transfection
chemical_list: D011073:Polyamines