SS-B/La antigen purification, and ELISA detection of anti-SS-B/La antibodies in sera from patients with inflammatory connective tissue diseases.
SS-B/La antigen was purified by immunoadsorbent columns with immunoglobulin from a patient with primary Sjögren's syndrome. Monitoring of the purification was facilitated by the fused rocket-immunoelectrophoresis technique. Technical ELISA variables for the detection of serum antibodies against the SS-B/La antigen were evaluated, and a recommended procedure is described. Prospective investigation of anti-SS-B/La antibodies in 103 blood donors and 131 patients with chronic inflammatory connective tissue diseases, including 43 patients with primary Sjögren's syndrome was performed. Anti-SS-B/La antibody concentrations were above normal in 65% of the patients with primary Sjögren's syndrome (verified by the strictly objective Copenhagen criteria for keratoconjunctivitis sicca and xerostomia) and 9% of patients with other chronic connective tissue diseases. The predictive value for primary Sjögren's syndrome among patients with increased levels of the anti-SS-B/La antibodies attending a rheumatology clinic was 78%.
Hansen
B
B
Department of Internal Medicine, University of Lund, Malmö General Hospital, Sweden.
Larsen
P
P
Manthorpe
R
R
D000974:Antibodies, Antinuclear / Q000032:analysis*; D001324:Autoantigens / Q000302:isolation & purification*; D003095:Collagen Diseases / Q000175:diagnosis*; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007122:Immunoelectrophoresis; D012261:Ribonucleoproteins; D012859:Sjogren's Syndrome / Q000175:diagnosis*; D014157:Transcription Factors / Q000302:isolation & purification; D000097585:SS-B Antigen
D000974:Antibodies, Antinuclear; D001324:Autoantigens; D012261:Ribonucleoproteins; D014157:Transcription Factors; D000097585:SS-B Antigen; C035357:SS-B antibodies