Native cytokines do not bind to uromodulin (Tamm-Horsfall glycoprotein).
Uromodulin bound with high affinity to human tumour necrosis factor (TNF) coated on microtitre plates. This interaction was not competitively inhibited by native TNF in solution. No interaction was observed between immobilized uromodulin and TNF in the liquid phase unless conditions were chosen which denatured the latter protein. Recombinant interleukin-1 alpha adsorbed on microtitre plates also interacted with uromodulin. However, gel filtration experiments demonstrated no interaction between the proteins in the liquid phase. These and additional results indicate that uromodulin interacts with denatured cytokines, but not with native, soluble cytokines.
Moonen
P
P
Glaxo Institute for Molecular Biology, Geneva, Switzerland.
Gaffner
R
R
Wingfield
P
P
D003115:Colony-Stimulating Factors / Q000378:metabolism; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D006133:Growth Substances / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007375:Interleukin-1 / Q000378:metabolism; D007700:Kinetics; D008297:Male; D009088:Mucoproteins / Q000378:metabolism*; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D058949:Uromodulin
D003115:Colony-Stimulating Factors; D006133:Growth Substances; D007375:Interleukin-1; D009088:Mucoproteins; D011257:Pregnancy Proteins; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; C548612:UMOD protein, human; D058949:Uromodulin; D016178:Granulocyte-Macrophage Colony-Stimulating Factor