Interleukin-2 receptor is functionally linked with in vitro activation of B cells isolated from non-Hodgkin's lymphomas.
Proliferation and differentiation of B cells can be promoted by a number of inducing factors, including interleukin-2 (IL-2). In vitro-stimulated normal B cells usually express IL-2 receptors (TAC). Furthermore, it has been reported that some minor sub-populations of B cells in normal lymphoid tissue also express TAC antigen, as do some leukemic B cells. In the present study, the reactivity of 20 specimens of non-Hodgkin's lymphoma or B-cell origin was examined for a wide panel of monoclonal antibodies (MAb), including an anti-TAC MAb. The ability of the isolated and purified B cells to proliferate in the presence of B-cell mitogens and culture supernatants (containing various growth factors, including IL-2) was also investigated. These experiments provided some evidence that the TAC-negative cells remained unreactive, whereas the TAC-positive cells entered the cell cycle and proliferated. IL-2 responsiveness of neoplastic B cells is therefore discussed.
Garraud
O
O
Department of Haematology and Clinical Oncology, Fondation Bergonie, Bordeaux, France.
LeGrand
E
E
Eghbali
H
H
Hoerni-Simon
G
G
Hoerni
B
B
Guillemain
B
B
D000911:Antibodies, Monoclonal; D001402:B-Lymphocytes / Q000378:metabolism*; D002455:Cell Division; D002457:Cell Extracts; D002478:Cells, Cultured; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology; D008228:Lymphoma, Non-Hodgkin / Q000175:diagnosis*; D008297:Male; D008934:Mitogens / Q000494:pharmacology; D015375:Receptors, Interleukin-2 / Q000276:immunology*
D000911:Antibodies, Monoclonal; D002457:Cell Extracts; D007376:Interleukin-2; D008934:Mitogens; D015375:Receptors, Interleukin-2