The predicted DBL oncogene product defines a distinct class of transforming proteins.
The DBL transforming gene was originally identified by transfection of NIH 3T3 cells with DNA from a human B-cell lymphoma. This gene was found to have arisen as a result of recombination of the 3' portion of the DBL protooncogene coding sequences with an unrelated segment of human DNA. It encodes a cytoplasmic protein that is equally distributed between cytosol and crude membrane fractions. To further characterize this transforming gene, a biologically active cDNA clone of the DBL transforming gene mRNA was isolated. Analysis of the sequence of the DBL oncogene cDNA revealed a long open reading frame that encodes a hybrid protein whose first 50 amino acids (at least) derive from a complete exon of a different locus. No significant homology with known oncogenes or any known protein sequences was demonstrated. The computer analysis of the predicted DBL protein indicated it is highly hydrophilic with no hydrophobic domains characteristic of a membrane-spanning region or signal peptide. Thus, the DBL oncoprotein is distinct among known transforming gene products.
Eva
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Laboratory of Cellular and Molecular Biology, National Cancer Institute, Bethesda, MD 20892.
Vecchio
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G
Rao
C D
CD
Tronick
S R
SR
Aaronson
S A
SA
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D004247:DNA / Q000235:genetics; D004273:DNA, Neoplasm / Q000235:genetics*; D005347:Fibroblasts; D020662:Guanine Nucleotide Exchange Factors; D006801:Humans; D008223:Lymphoma / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D009857:Oncogenes; D011518:Proto-Oncogene Proteins / Q000145:classification*; D011993:Recombinant Fusion Proteins; D012045:Regulatory Sequences, Nucleic Acid; D012191:Retroviridae Proteins / Q000235:genetics*; D015682:Retroviridae Proteins, Oncogenic; D014170:Transformation, Genetic
D004273:DNA, Neoplasm; D020662:Guanine Nucleotide Exchange Factors; C057811:MCF2 protein, human; C512843:Mcf2 protein, mouse; D011518:Proto-Oncogene Proteins; D011993:Recombinant Fusion Proteins; D012191:Retroviridae Proteins; D015682:Retroviridae Proteins, Oncogenic; D004247:DNA