Genotypic analysis using a Y-chromosome-specific probe following bone marrow transplantation.
To monitor successful engraftment after bone marrow transplantation, we performed Southern hybridization analysis or dot blot analysis of DNA in a set of sex-mismatched cases using a Y-chromosome-specific DNA probe (pHY10). This method was extremely sensitive and rapid for checking which cells contain the Y-chromosome. Using this probe, analysis of cells from peripheral blood and bone marrow after transplantation demonstrated the usefulness of confirming engraftment of donor cells and of detecting mixed lymphohematopoietic chimerism.
Morisaki
H
H
Department of Pathological Pharmacology, University of Tokyo, Japan.
Morisaki
T
T
Nakahori
Y
Y
Ogura
H
H
Kanno
H
H
Tani
K
K
Kodo
H
H
Fujii
H
H
Asano
S
S
Miwa
S
S
D000328:Adult; D001853:Bone Marrow / Q000032:analysis; D016026:Bone Marrow Transplantation; D004247:DNA / Q000032:analysis; D005260:Female; D005838:Genotype; D006801:Humans; D007951:Leukemia, Myeloid / Q000097:blood / Q000235:genetics / Q000628:therapy; D015470:Leukemia, Myeloid, Acute / Q000097:blood / Q000235:genetics / Q000628:therapy; D008297:Male; D009693:Nucleic Acid Hybridization; D014998:Y Chromosome
D004247:DNA