title: An immunogold cell labelling method for rat T lymphocytes.
abstract: A method using monoclonal antibodies in conjunction with an immunogold procedure was adapted for labelling T lymphocytes in blood samples obtained from male Wistar rats. The monoclonal antibodies W3/25, MRC/OX8 and W3/13 were used. Changes in the percentages of positively labelled cells were observed in rats dosed with the immunosuppressant cyclosporin.
authors.affiliation: Department of Toxicology and Metabolism, Wellcome Research Laboratories, Beckenham, Kent, UK.
authors.forename: G O
authors.initials: GO
authors.lastname: Evans
authors.forename: R M
authors.initials: RM
authors.lastname: Flynn
authors.forename: J D
authors.initials: JD
authors.lastname: Lupton
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D003524:Cyclosporins / Q000494:pharmacology; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D051381:Rats; D011919:Rats, Inbred Strains / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D003524:Cyclosporins