title: Quantitative basic residue requirements in the cleavage-activation site of the fusion glycoprotein as a determinant of virulence for Newcastle disease virus.
abstract: Newcastle disease virus exhibits a wide range of pathogenicity and virulence which, as with all paramyxoviruses, is directly related to the cleavability of a precursor (F0) of the fusion glycoprotein by cellular proteases. Sequence analyses of the cleavage site of several virulent and avirulent isolates of the Newcastle disease virus serotype reveal a correlation between virulence or pathogenicity and a high content of basic amino acid residues at the cleavage site. A similar correlation has been seen for other paramyxoviruses.
authors.affiliation: Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester 01605.
authors.forename: R L
authors.initials: RL
authors.lastname: Glickman
authors.forename: R J
authors.initials: RJ
authors.lastname: Syddall
authors.forename: R M
authors.initials: RM
authors.lastname: Iorio
authors.forename: J P
authors.initials: JP
authors.lastname: Sheehan
authors.forename: M A
authors.initials: MA
authors.lastname: Bratt
mesh_terms: D000595:Amino Acid Sequence; D009522:Newcastle disease virus / Q000378:metabolism / Q000472:pathogenicity*; D010447:Peptide Hydrolases / Q000378:metabolism; D011498:Protein Precursors / Q000378:metabolism; D013329:Structure-Activity Relationship; D014760:Viral Fusion Proteins / Q000378:metabolism*
chemical_list: D011498:Protein Precursors; D014760:Viral Fusion Proteins; D010447:Peptide Hydrolases