The enzymatic and mass spectrometric identification of 2-oxophytanic acid, a product of the peroxisomal oxidation of l-2-hydroxyphytanic acid.
A previously unreported metabolite of the mammalian phytanic acid breakdown pathway, 2-oxophytanic acid, was isolated and analysed by mass spectrometry. The metabolic origin of the 2-oxoacid is the oxidation by a rat kidney peroxisomal H2O2-generating oxidase of L-2-hydroxyphytanic acid, a well-established intermediate in phytanic acid alpha-oxidation.
Vamecq
J
J
Laboratoire de Chimie Physiologique, International Institute of Cellular and Molecular Pathology, Brussels, Belgium.
Draye
J P
JP
D000429:Alcohol Oxidoreductases / Q000032:analysis; D000818:Animals; D002849:Chromatography, Gas; D004537:Eicosanoic Acids / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry; D007668:Kidney / Q000378:metabolism*; D007672:Kidney Cortex / Q000201:enzymology / Q000378:metabolism; D008297:Male; D008830:Microbodies / Q000378:metabolism*; D010084:Oxidation-Reduction; D010831:Phytanic Acid / Q000031:analogs & derivatives / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains
D004537:Eicosanoic Acids; D010831:Phytanic Acid; C002585:2-hydroxyphytanic acid; C056198:2-oxophytanic acid; D000429:Alcohol Oxidoreductases; C056199:2-hydroxyphytanate oxidase