Ancient heat shock gene is dispensable.
Hsp83 is a major eucaryotic heat shock protein and one of the most conserved proteins known. We have isolated an Escherichia coli gene homologous to eucaryotic Hsp83 and used it to construct a deletion mutation. The E. coli mutant was viable but had a slight growth disadvantage that increased with temperature.
Bardwell
J C
JC
Department of Physiological Chemistry, University of Wisconsin Medical School, Madison 53706.
Craig
E A
EA
D002872:Chromosome Deletion; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development; D006360:Heat-Shock Proteins / Q000096:biosynthesis / Q000235:genetics*; D006358:Hot Temperature; D009154:Mutation; D009693:Nucleic Acid Hybridization; D010957:Plasmids
D004269:DNA, Bacterial; D006360:Heat-Shock Proteins