In vitro models of differentiated Sertoli cell structure and function.
Primary cultures of Sertoli cells maintained in conventional cultures on plastic culture vessels do not retain many of the structural and functional properties of their in vivo counterparts. Sertoli cell phenotype is better maintained by incorporating certain environmental parameters, intrinsic to the testis, into the Sertoli cell culture system. These environmental parameters include a) high cell density, b) a unique extracellular matrix, c) a semipermeable support between the basal plasma membrane of the cells and blood-derived nutrients in the interstitium, d) chemically distinct microenvironments at the apical and basal surfaces of the cells, and e) cell-to-cell interactions among Sertoli cells and other testicular cell types. Using three variations of Sertoli cell culture we have demonstrated the importance of each of these environmental parameters in obtaining a better Sertoli cell culture model.
Hadley
M A
MA
Department of Anatomy and Cell Biology, Georgetown Medical School, Washington, DC 20007.
Byers
S W
SW
Suárez-Quian
C A
CA
Djakiew
D
D
Dym
M
M
D000818:Animals; D001485:Basement Membrane / Q000502:physiology; D002450:Cell Communication; D002454:Cell Differentiation; D002478:Cells, Cultured; D005109:Extracellular Matrix / Q000502:physiology; D008297:Male; D012708:Sertoli Cells / Q000502:physiology / Q000648:ultrastructure; D013737:Testis / Q000502:physiology