A comparison of methods for analysis of mRNA in hematopoietic cells: conventional and clonal northern analysis and in situ hybridization.
Three methods for the detection of mRNA in hematopoietic cells are addressed, and examples using these techniques to examine human leukemic cells' genetic response to chemical and biological response modifiers shown. The limitations and specific applications of the three techniques are discussed.
Castiglia
C L
CL
Sloan-Kettering Institute, New York, N.Y.
D015152:Blotting, Northern; D002460:Cell Line; D002999:Clone Cells / Q000502:physiology; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D006801:Humans; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000032:analysis*; D015375:Receptors, Interleukin-2 / Q000235:genetics
D012333:RNA, Messenger; D015375:Receptors, Interleukin-2