[Enzyme immunological measurement of estrogen receptors in the human endometrium by the use of monoclonal antibody].
The concentrations of cytosol (ERc), nuclear (ERn), and total (ERc+n) estrogen receptors were determined by Enzyme Immunoassay using Monoclonal Antibody, in normal endometrium freshly obtained from 13 cases, and were compared with those determined by a conventional ligand binding assay on LH-20 (LH-20 assay). There was good correlation between EIA and LH-20 values. The correlation coefficients for ERc, ERn and ERc+n were 0.960, 0.980 and 0.950, respectively. Regression lines were as follows; Ec = 1.22Lc-4.07, En = 1.18Ln+37.7 and Ec+n = 1.27 Lc+n-158.4. These results clearly demonstrate the applicability of the ER-EIA for the determination of estrogen receptors in the endometrium. Particularly for clinical laboratory purposes, the enzyme immunoassay has some advantages over the conventional binding assay in terms of non-radioactivity and simple procedure.
Kato
J
J
Department of Obstetrics and Gynecology, Yamanashi Medical College.
Hirata
S
S
D000328:Adult; D000911:Antibodies, Monoclonal; D004717:Endometrium / Q000032:analysis*; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D008875:Middle Aged; D011869:Radioligand Assay; D011960:Receptors, Estrogen / Q000032:analysis*
D000911:Antibodies, Monoclonal; D011960:Receptors, Estrogen