S100a0 (alpha alpha) protein in cardiac muscle. Isolation from human cardiac muscle and ultrastructural localization.
S100 protein, an acidic and calcium-binding protein, was believed to be localized in the nervous tissue, but recently it has been reported to be mainly present in the cardiac and the skeletal muscles of various mammals in the alpha alpha form (S100a0) at much higher levels than the nervous tissues. We isolated here S100 protein from human cardiac muscles. The isolated cardiac muscle S100 protein showed a single band on electrophoresis at the same position as that of human skeletal muscle S100a0. The amino acid composition of the purified S100 protein was quite similar to that of human skeletal muscle S100a0 or bovine brain S100a0. The immunohistochemical study by use of antibodies monospecific to the alpha subunit of S100 protein (S100-alpha) revealed that S100-alpha was strongly labeled in human myocardial cells, whereas the beta subunit of S100 protein (S100-beta) was not detected in the cells. These results suggest that a predominant form of S100 protein in human myocardial cells is not S100a (alpha beta) or S100b (beta beta), but S100a0 (alpha alpha). In order to determine the ultrastructural localization of S100a0 in mouse cardiac muscle, the direct peroxidase-labeled antibody method was employed. S100a0 was mainly localized in the polysomes in the interfibrillar spaces, the fine filamentous structure of the Z line and fascia adherens of the intercalated disc and in the lumen of junctional sarcoplasmic reticulum.
Haimoto
H
H
Laboratory of Pathology, Aichi Cancer Center Research Institute, Japan.
Kato
K
K
D002135:Calcium-Binding Proteins / Q000302:isolation & purification* / Q000378:metabolism; D002451:Cell Compartmentation; D006801:Humans; D007158:Immunologic Techniques; D046911:Macromolecular Substances; D008854:Microscopy, Electron; D009206:Myocardium / Q000032:analysis* / Q000648:ultrastructure; D009418:S100 Proteins / Q000302:isolation & purification* / Q000378:metabolism
D002135:Calcium-Binding Proteins; D046911:Macromolecular Substances; D009418:S100 Proteins