Immunoperoxidase localization of apolipoprotein D in human enterocytes and hepatocytes.
In this study we prepared a pure apolipoprotein D and obtained a specific antiserum to it. The purified apolipoprotein D migrated as a single band of Mr = 29,000 but appeared as five isoforms on isoelectrofocusing. The antiserum did not cross-react with other apolipoproteins. Immunoenzymatic staining revealed the presence of apolipoprotein D in the perinuclear area of the cytoplasm of isolated normal hepatocytes and HepG2 cells. Apolipoprotein D was also localized in intestinal epithelium and in liver cells. The intracellular distribution of apolipoprotein D was similar to that of apolipoprotein B. Our results indicated that apolipoprotein D, like many other circulating apolipoproteins, is synthesized in enterocytes and hepatocytes.
Bouma
M E
ME
INSERM Unité 9, Hôpital Saint-Antoine, Paris, France.
de Bandt
J P
JP
Ayrault-Jarrier
M
M
Burdin
J
J
Verthier
N
N
Raisonnier
A
A
D000328:Adult; D001053:Apolipoproteins / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D053399:Apolipoproteins D; D006801:Humans; D007106:Immune Sera; D007124:Immunoenzyme Techniques; D007413:Intestinal Mucosa / Q000166:cytology / Q000378:metabolism*; D008099:Liver / Q000166:cytology / Q000378:metabolism*
D001053:Apolipoproteins; D053399:Apolipoproteins D; D007106:Immune Sera