title: Biochemistry of fusion mass consolidation in the sheep spine.
abstract: We report here the biochemistry of fusion mass consolidation in sheep spines during a 1-year period following autogenous cortical-cancellous bone grafting and stabilization with Harrington distraction rods. Biochemical analysis of vertebral fusion mass included determination of wet weight and dry weight and quantification of glycosaminoglycan, collagen, calcium, and phosphate following extraction with neutral EDTA and proteolytic hydrolysis with papain. Our results showed that at 1 week after surgery, the fusion mass consisted of original cortical and cancellous bone graft material. The cortical bone graft was partially resistant to EDTA-papain treatment, resulting in a residue containing hydroxyproline and mineral. At 12 weeks after surgery, the fusion mass had become a homogeneous material, which, like cancellous bone graft, was completely susceptible to treatment by EDTA-papain. Collagen content of consolidating fusion mass was highest at 16 weeks after surgery when normalized to dry weight; glycosaminoglycan content was highest within 6 weeks after surgery. Mineral content was lowest at the 6-week stage but by 12 weeks after surgery, it was comparable with original bone grafting material. At 24 and 52 weeks after surgery, fusion mass consolidation was characterized by an increase in the proportion of organic and mineral components resistant to EDTA-papain. The appearance of the EDTA-papain-resistant material in the fusion mass coincided with formation of lamellar bone and successful consolidation.
authors.affiliation: Orthopedic Research Laboratory, Stanford University, School of Medicine, California.
authors.forename: R
authors.initials: R
authors.lastname: Slater
authors.forename: D
authors.initials: D
authors.lastname: Nagel
authors.forename: R L
authors.initials: RL
authors.lastname: Smith
mesh_terms: D000818:Animals; D016025:Bone Transplantation; D001842:Bone and Bones / Q000378:metabolism*; D002118:Calcium / Q000032:analysis; D005260:Female; D006025:Glycosaminoglycans / Q000032:analysis; D006909:Hydroxyproline / Q000032:analysis; D009984:Orthopedic Fixation Devices; D010710:Phosphates / Q000032:analysis; D012756:Sheep; D013123:Spinal Fusion; D013997:Time Factors; D014945:Wound Healing
chemical_list: D006025:Glycosaminoglycans; D010710:Phosphates; D006909:Hydroxyproline; D002118:Calcium