Cloning and expression of the Escherichia coli K-12 sad gene.
The Escherichia coli K-12 sad gene, which encodes an NAD-dependent succinic semialdehyde dehydrogenase, was cloned into a high-copy-number vector. Minicells carrying a sad+ plasmid produced a 55,000-dalton peptide, the probable sad gene product.
Marek
L E
LE
Department of Microbiology, Montana State University, Bozeman 59717.
Henson
J M
JM
D001426:Bacterial Proteins / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003360:Cosmids; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005786:Gene Expression Regulation; D005798:Genes, Bacterial; D006884:Hydroxybutyrate Dehydrogenase / Q000096:biosynthesis / Q000235:genetics*; D009154:Mutation; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D005680:gamma-Aminobutyric Acid / Q000031:analogs & derivatives / Q000378:metabolism
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D005680:gamma-Aminobutyric Acid; D006884:Hydroxybutyrate Dehydrogenase; C019416:4-hydroxybutyrate dehydrogenase; C009338:succinic semialdehyde