Assaying the quality of cDNA libraries.
Once a cDNA library has been constructed, it is very useful to be able to easily assess the quality of the library. Because actin is a ubiquitous sequence, this assessment has been accomplished by probing filter lifts and Southern blots of libraries with an actin cDNA probe. These methods provide information about the percent of actin-positive clones and the degree of completeness of cDNA clones in a library. Results of these methods are correlated with the success of finding full-length clones of interest.
Hagen
F S
FS
ZymoGenetics, Inc., Seattle, WA 98105.
Gray
C L
CL
Kuijper
J L
JL
D000199:Actins / Q000235:genetics; D015152:Blotting, Northern; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis*; D015723:Gene Library; D011786:Quality Control
D000199:Actins; D004247:DNA