[Comparison of the binding nature of mibolerone to androgen receptor of human prostate with that of R1881].
The authors examined the binding nature of mibolerone in cytosols of hypertrophic prostates from 15 patients to androgen receptor using Dextran-charcoal assay, analyzed it by the method of Scatchard, and compared it with that of R1881. The addition of triamcinolone acetonide into the incubation medium induced specific single binding of mibolerone to androgen receptor with high affinity as well as R1881. The receptor contents obtained with mibolerone were higher than those of R1881, and both of them correlated well. The dissociation constants of both ligands showed good correlation and no significant differences. Mibolerone seems to be as suitable a ligand as R1881 for measuring the androgen receptor.
Miki
H
H
2nd Department of Surgery, School of Medicine, University of Tokushima, Japan.
Oshimo
K
K
Inoue
H
H
Sumi
T
T
Yamashita
K
K
Morimoto
T
T
Monden
Y
Y
D004963:Estrenes / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D015741:Metribolone; D009277:Nandrolone / Q000031:analogs & derivatives* / Q000378:metabolism; D011467:Prostate / Q000032:analysis*; D011944:Receptors, Androgen / Q000032:analysis* / Q000378:metabolism; D014221:Triamcinolone / Q000494:pharmacology
D004963:Estrenes; D011944:Receptors, Androgen; D014221:Triamcinolone; D015741:Metribolone; D009277:Nandrolone; C100075:mibolerone