Identification and localization of proteins encoded by two DIF-inducible genes of Dictyostelium.
We show that pDd56 and pDd63, two related DIF-inducible genes of Dictyostelium, respectively encode the ST310 and ST430 polypeptides identified by Morrissey, Devine, and Loomis (1984, Dev. Biol. 103, 414-424). We localize the two proteins by immunoelectron microscopy to the extracellular matrix surrounding the stalk cells and the stalk tube. Coupled with their predicted amino acid sequence and biochemical properties, this suggests that they are structural proteins of the stalk.
McRobbie
S J
SJ
Imperial Cancer Research Fund, Clare Hall Laboratories, Hertfordshire, United Kingdom.
Tilly
R
R
Blight
K
K
Ceccarelli
A
A
Williams
J G
JG
D004023:Dictyostelium / Q000235:genetics* / Q000648:ultrastructure; D005109:Extracellular Matrix / Q000032:analysis / Q000648:ultrastructure; D005656:Fungal Proteins / Q000032:analysis / Q000235:genetics*; D005786:Gene Expression Regulation / Q000187:drug effects; D005800:Genes, Fungal; D006588:Hexanones / Q000494:pharmacology*; D007163:Immunosorbent Techniques; D007659:Ketones / Q000494:pharmacology*; D008854:Microscopy, Electron
D005656:Fungal Proteins; D006588:Hexanones; D007659:Ketones; C053433:1-((3,5-dichloro)-2,6-dihydroxy-4-methoxyphenyl)-1-hexanone