title: Minimal chronic hyperglycemia is a critical determinant of impaired insulin secretion after an incomplete pancreatectomy.
abstract: We now describe experiments that allow one to determine the consequences of B cell reduction alone vs. those that result from superimposed mild hyperglycemia. Male CD rats underwent a 60% pancreatectomy (Px); controls were sham operated. 1 wk later, either 10% sucrose (SUC) was substituted as fluid supply or tap water was continued (WAT). Plasma glucose and insulin values in Px-WAT remained equal to the sham groups; in Px-SUC the values were euglycemic for 25 d, but then nonfasting plasma glucose rose 15 mg/dl. After 6 wk, B cell mass in Px-WAT was reduced by 45% and non-B cell mass by 57%. In contrast, in Px-SUC both masses were comparable to the sham groups. The insulin response to 27.7 mM glucose was measured using the in vitro perfused pancreas. The responses were reduced in Px-WAT but in proportion to their reduced B cell mass; in contrast, it was 75% less than expected in Px-SUC. Also, the response to arginine given at 16.7 mM glucose was reduced only in Px-SUC. These results show that a lowering of B cell mass that does not result in hyperglycemia has no adverse effect on the remaining B cells. On the other hand, if even mild hyperglycemia develops, B cell function becomes impaired and results in inappropriately reduced insulin stores and insulin responses to marked stimuli.
authors.affiliation: Research Division, Joslin Diabetes Center, Boston, MA 02215.
authors.forename: J L
authors.initials: JL
authors.lastname: Leahy
authors.forename: S
authors.initials: S
authors.lastname: Bonner-Weir
authors.forename: G C
authors.initials: GC
authors.lastname: Weir
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D001835:Body Weight; D002908:Chronic Disease; D004326:Drinking; D006943:Hyperglycemia / Q000209:etiology / Q000503:physiopathology*; D007328:Insulin / Q000032:analysis / Q000097:blood / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000032:analysis / Q000378:metabolism* / Q000503:physiopathology; D008297:Male; D010180:Pancreatectomy / Q000009:adverse effects*; D051381:Rats
chemical_list: D001786:Blood Glucose; D007328:Insulin