[Genetic engineering of peptide hormones. III. Cloning of the swine growth hormone cDNA and construction of the gene for expression of the hormone in bacteria].
The clones containing cDNA of porcine growth hormone were obtained using poly(A)-RNA from porcine pituitary as a template for reverse transcriptase. The analysis of their nucleotide sequences revealed that these cDNAs have differences not only on the nucleotide level but also on the amino acid level, i. e. the polymorphism of mRNA and protein occurs in the case of porcine growth hormone. To create the construction for expression of porcine growth hormone in E. coli, the 5'-part of cDNA, coding the first 15 amino acids of the mature hormone, was substituted by the artificial sequence.
Zhvirblis
G S
GS
Gorbulev
V G
VG
Rubtsov
P M
PM
Chernov
B K
BK
Golova
Iu B
IuB
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D005813:Genes, Synthetic; D005822:Genetic Vectors; D013006:Growth Hormone / Q000235:genetics*; D008969:Molecular Sequence Data; D021382:Protein Sorting Signals / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics; D013552:Swine
D021382:Protein Sorting Signals; D011994:Recombinant Proteins; D013006:Growth Hormone; D004247:DNA
Geneticheskaia inzheneriia peptidnykh gormonov. III. Klonirovanie kDNK gormona rosta svin'i i konstruirovanie gena dlia ékspressii gormona v bakteriiakh.