title: Detection of dengue virus type 2 in Aedes albopictus by nucleic acid hybridization with strand-specific RNA probes.
abstract: A molecular hybridization technique with radiolabeled, strand-specific RNA probes was developed to detect dengue virus type 2 RNA in pools of infected Aedes albopictus mosquitoes. One infected mosquito in a pool of 25 could be detected, corresponding to a dengue virus type 2 titer of 2.75 log10 50% tissue culture infectious doses.
authors.affiliation: Department of Microbiology, Colorado State University, Fort Collins 80523.
authors.forename: K
authors.initials: K
authors.lastname: Olson
authors.forename: C
authors.initials: C
authors.lastname: Blair
authors.forename: R
authors.initials: R
authors.lastname: Padmanabhan
authors.forename: B
authors.initials: B
authors.lastname: Beaty
mesh_terms: D000330:Aedes / Q000382:microbiology*; D000818:Animals; D003716:Dengue Virus / Q000235:genetics / Q000302:isolation & purification*; D005260:Female; D005455:Fluorescent Antibody Technique; D007303:Insect Vectors / Q000382:microbiology*; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000032:analysis*
chemical_list: D012367:RNA, Viral