IL-2-PE40 is cytotoxic for activated T lymphocytes expressing IL-2 receptors.
IL-2-PE40 is a chimeric molecule in which IL-2 is attached to the amino end of modified Pseudomonas exotoxin molecule lacking cell recognition domain. This molecule was extremely toxic for Con A-stimulated spleen cells from mice. Moreover, IL-2-PE40 has suppressive effect against Ag-activated cells; it inhibits the generation of cytotoxic T lymphocyte activity in a MLC. IL-2-PE40 could be a useful agent in IL-2R targeting therapy including immunosuppressive therapy for allograft rejection or some autoimmune diseases.
Ogata
M
M
Laboratory of Molecular Biology, National Cancer Institute, Bethesda, MD 20892.
Lorberboum-Galski
H
H
FitzGerald
D
D
Pastan
I
I
D000818:Animals; D001426:Bacterial Proteins / Q000633:toxicity; D003208:Concanavalin A; D005098:Exotoxins / Q000633:toxicity*; D007166:Immunosuppressive Agents / Q000633:toxicity; D000922:Immunotoxins / Q000633:toxicity*; D007376:Interleukin-2 / Q000633:toxicity*; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D011500:Protein Synthesis Inhibitors / Q000633:toxicity; D011549:Pseudomonas / Q000276:immunology*; D015375:Receptors, Interleukin-2 / Q000187:drug effects*; D011994:Recombinant Proteins / Q000633:toxicity*; D013154:Spleen; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000378:metabolism
D001426:Bacterial Proteins; D005098:Exotoxins; C057961:IL-2-PE40 chimeric protein, recombinant; D007166:Immunosuppressive Agents; D000922:Immunotoxins; D007376:Interleukin-2; D011500:Protein Synthesis Inhibitors; D015375:Receptors, Interleukin-2; D011994:Recombinant Proteins; D003208:Concanavalin A