Use of immunosorbent-purified antigens of Fasciola hepatica in enzyme immunoassays.
A monoclonal antibody specific for the T1 tegumental antigen of Fasciola hepatica was used as a solid-phase immunosorbent for the purification of T1 antigen from homogenised mature F hepatica. Material fractionated by this technique was successfully used in enzyme-linked immunoassays to detect antibodies to F hepatica in sera from sheep and cattle. Species differences in response to infection by F hepatica were demonstrated.
Trudgett
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Department of Biology, Queen's University of Belfast, Northern Ireland.
Anderson
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Hanna
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D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000947:Antigens, Helminth / Q000276:immunology* / Q000302:isolation & purification; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis*; D004797:Enzyme-Linked Immunosorbent Assay; D005210:Fasciola hepatica / Q000276:immunology*; D005211:Fascioliasis / Q000175:diagnosis / Q000662:veterinary*; D007163:Immunosorbent Techniques; D012756:Sheep; D012757:Sheep Diseases / Q000175:diagnosis*
D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth