Cultivation on cellophane agar as a method for the assay of bacterial enzymes.
Production of bacterial enzymes in a liquid medium and on cellophane-agar plates is compared. Using neutral proteinase, urease, alpha-amylase and thiosulfate sulfurtransferase as examples it was demonstrated that the cultivation on cellophane-agar plates yields higher concentrations of the enzymes and that the enzyme production is detected in a higher number of cultures. The method might be useful for the characterization of bacterial communities.
Vraný
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Institute of Sera and Vaccines, Jevany-Bohumile, Czechoslovakia.
Hnátková
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Z
D000362:Agar; D001419:Bacteria / Q000201:enzymology* / Q000254:growth & development; D001431:Bacteriological Techniques; D002476:Cellophane; D003470:Culture Media; D008666:Metalloendopeptidases / Q000096:biosynthesis; D013884:Thiosulfate Sulfurtransferase / Q000096:biosynthesis; D014510:Urease / Q000096:biosynthesis; D000516:alpha-Amylases / Q000096:biosynthesis
D003470:Culture Media; D000362:Agar; D002476:Cellophane; D013884:Thiosulfate Sulfurtransferase; D000516:alpha-Amylases; D008666:Metalloendopeptidases; C052706:neutral proteinase, cervical; D014510:Urease