title: Thrombospondin is present in articular cartilage and is synthesized by articular chondrocytes.
abstract: Thrombospondin, a multifunctional adhesive glycoprotein originally identified in platelets, was isolated and identified from an extract of ovine articular cartilage. Immunoreactive material from a cartilage extract comigrated on gel electrophoresis with purified human platelet thrombospondin. When articular chondrocytes were cultured in the presence of 35S-methionine, metabolically labeled thrombospondin was immunoprecipitated from the culture medium and cell layer extract. These results demonstrate that thrombospondin is present in articular cartilage and is synthesized by articular chondrocytes.
authors.affiliation: Department of Musculoskeletal Research, Cleveland Clinic Foundation Research Institute 44195.
authors.forename: R R
authors.initials: RR
authors.lastname: Miller
authors.forename: C A
authors.initials: CA
authors.lastname: McDevitt
mesh_terms: D000818:Animals; D002358:Cartilage, Articular / Q000378:metabolism*; D002478:Cells, Cultured; D011232:Chemical Precipitation; D002846:Chromatography, Affinity; D006023:Glycoproteins / Q000378:metabolism*; D007163:Immunosorbent Techniques; D066298:In Vitro Techniques; D012756:Sheep; D019699:Thrombospondins
chemical_list: D006023:Glycoproteins; D019699:Thrombospondins