Construction of immunogens for synthetic malaria vaccines.
The immunogenicity of a peptide consisting of eight repeats of the tetrapeptide sequence NANP (Asn-Ala-Asn-Pro) contained in the circumsporozoite protein of Plasmodium falciparum was investigated in mice under different modes of presentation. This peptide was able to produce biologically active antibodies when administered with adjuvant and linked to a protein carrier. However, a (NANP) peptide polymerized by carbodiimide was found to be immunogenic in the absence of protein carrier in H-2b mice. In contrast, the (NANP)8 peptide polymerized by glutaraldehyde was not immunogenic in the same strain. Furthermore, the efficacy of murabutide in saline, as an immunological adjuvant, was compared to the efficacy of Freund's complete adjuvant.
Lise
D
D
Department of Pharmacology and Therapeutics, University of South Florida, College of Medicine, Tampa 33612.
Dubeaux
C
C
Tello
D
D
Mazier
D
D
Jolivet
M
M
Schlesinger
D H
DH
Audibert
F
F
Chedid
L
L
D000595:Amino Acid Sequence; D000818:Animals; D000917:Antibody Formation; D000953:Antigens, Protozoan; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D036341:Intercellular Signaling Peptides and Proteins; D008288:Malaria / Q000517:prevention & control*; D051379:Mice; D008815:Mice, Inbred Strains; D009842:Oligopeptides / Q000378:metabolism; D010455:Peptides / Q000276:immunology*; D010963:Plasmodium falciparum / Q000276:immunology*; D014612:Vaccines; D014614:Vaccines, Synthetic
D000953:Antigens, Protozoan; D036341:Intercellular Signaling Peptides and Proteins; D009842:Oligopeptides; D010455:Peptides; D014612:Vaccines; D014614:Vaccines, Synthetic; C056047:(asparaginyl--alanyl-asparaginyl-proline)8