Rabbit articular chondrocytes: an in vitro model for studying the effect of sodium aurothiopropanol sulfonate on proliferation kinetics, type II collagen phenotype and mitochondrial activity.
Despite the benefits of chrysotherapy the responsible mechanism of action of gold compounds remains unclear. At a concentration of 5 x 10(-4) M, sodium aurothiopropanol sulfonate (SAS) modified the in vitro proliferation kinetics of articular chondrocytes by reducing growth, viability and plating efficiency. Flow cytometry analysis, using propidium iodide DNA staining, revealed slight but significant cell arrest in G2+M which, in fact, represents an increase in the proportion of binucleate cells. SAS did not induce any variations in chondrocyte phenotype stability as far as the biosynthesis of type II collagen was concerned, and no appreciable changes in overall mitochondrial activity reflected by rhodamine 123 incorporation.
Ronot
X
X
Laboratoire de Pharmacologie Cellulaire de l'Ecole Pratique des Hautes Etudes, Paris, France.
Hainque
B
B
Christen
M O
MO
Froger
B
B
Hartmann
D J
DJ
Adolphe
M
M
Lechat
P
P
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D002135:Calcium-Binding Proteins / Q000096:biosynthesis* / Q000276:immunology; D002358:Cartilage, Articular / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis* / Q000276:immunology; D024043:Collagen Type II; D004112:Dimercaprol / Q000031:analogs & derivatives* / Q000494:pharmacology; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006046:Gold / Q000494:pharmacology; D066298:In Vitro Techniques; D008667:Metalloproteins / Q000494:pharmacology*; D008928:Mitochondria / Q000378:metabolism; D008938:Mitosis; D050607:Organogold Compounds; D009942:Organometallic Compounds; D020005:Propanols; D011498:Protein Precursors / Q000096:biosynthesis*; D011817:Rabbits; D013438:Sulfhydryl Compounds
D000894:Anti-Inflammatory Agents, Non-Steroidal; D002135:Calcium-Binding Proteins; D024043:Collagen Type II; D008667:Metalloproteins; D050607:Organogold Compounds; D009942:Organometallic Compounds; D020005:Propanols; D011498:Protein Precursors; D013438:Sulfhydryl Compounds; C040072:chondrocalcin; D004112:Dimercaprol; D006046:Gold; D003094:Collagen; C013394:aurotioprol