Expression of the FGF-related proto-oncogene int-2 during gastrulation and neurulation in the mouse.
The proto-oncogene int-2 has been implicated in the formation of mouse mammary-tumour-virus-induced mammary tumours. Analysis of the predicted coding sequence indicates that int-2 is a member of the fibroblast growth factor family. Previous studies using Northern blot analysis suggested that normal expression of int-2 may be confined to extra-embryonic endoderm lineages of embryonic stages of mouse development. We have used in situ hybridization and Northern blot analysis to examine directly int-2 expression in embryo stem cells and in the developing embryo from early gastrulation to midsomite stages. Complex patterns of accumulation of int-2 RNA were observed in embryonic and extra-embryonic tissues. The data suggest multiple roles for int-2 in development which may include migration of early mesoderm cells and induction of the otocyst.
Wilkinson
D G
DG
Laboratory of Developmental Biochemistry, National Institute for Medical Research, Mill Hill, London, UK.
Peters
G
G
Dickson
C
C
McMahon
A P
AP
D000818:Animals; D001921:Brain / Q000196:embryology / Q000378:metabolism; D004622:Embryo, Mammalian / Q000378:metabolism*; D004707:Endoderm / Q000378:metabolism; D005346:Fibroblast Growth Factors / Q000502:physiology*; D005786:Gene Expression Regulation; D008648:Mesoderm / Q000378:metabolism; D051379:Mice; D008808:Mice, Inbred CBA; D009693:Nucleic Acid Hybridization; D011518:Proto-Oncogene Proteins / Q000378:metabolism* / Q000502:physiology; D012333:RNA, Messenger / Q000378:metabolism* / Q000502:physiology
D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D005346:Fibroblast Growth Factors