Immunological identification of rat urinary inactive kallikrein as a proform of tissue kallikrein.
Antibody was raised against a synthetic undecapeptide (PS 11) which corresponds to the prosegment of the rat tissue kallikrein precursor. The potential to recognize rat urinary active or inactive kallikrein was assessed by an enzyme immunoassay method for PS 11, using beta-D-galactosidase as the labeling enzyme. The active kallikrein failed to compete with the enzyme-labeled PS 11 in binding to the antibody. The inactive kallikrein displaced the enzyme-labeled PS 11 in this enzyme immunoassay, and the displacement curve was in parallel with that of PS 11. These results indicate that rat urinary inactive kallikrein contains a prosequence recognized by the antibody to PS 11. This inactive kallikrein is probably a proform of tissue kallikrein.
Takaoka
M
M
Department of Pharmacology, Osaka University of Pharmaceutical Sciences, Japan.
Nakamura
M
M
Nishii
M
M
Kashiwagi
Y
Y
Morimoto
S
S
D000818:Animals; D000915:Antibody Affinity; D001667:Binding, Competitive; D004792:Enzyme Precursors / Q000276:immunology / Q000652:urine*; D007120:Immunochemistry; D007124:Immunoenzyme Techniques; D007610:Kallikreins / Q000276:immunology / Q000652:urine*; D051381:Rats
D004792:Enzyme Precursors; D007610:Kallikreins