title: The Kluyveromyces lactis KEX1 gene encodes a subtilisin-type serine proteinase.
abstract: KEX1 is a chromosomal gene required for the production of the killer toxin encoded by the linear DNA plasmid pGKL-1 of Kluyveromyces lactis. The nucleotide sequence of the cloned KEX1 gene has been determined. The deduced structure of the KEX1 protein, 700 amino acids long, indicated that it contained an internal domain with a striking homology to the sequences of the subtilisin-type proteinases, and a probable transmembrane domain near the carboxyl terminus. The results confirm the hypothesis that the product of the gene KEX1 of K. lactis is a proteinase involved in the processing of the toxin precursor.
authors.affiliation: Institut Curie, Section de Biologie, Centre Universitaire, Orsay, France.
authors.forename: C
authors.initials: C
authors.lastname: Tanguy-Rougeau
authors.forename: M
authors.initials: M
authors.lastname: Wésolowski-Louvel
authors.forename: H
authors.initials: H
authors.lastname: Fukuhara
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D005796:Genes; D005798:Genes, Bacterial; D007716:Kluyveromyces / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D004718:Saccharomycetales / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D012697:Serine Endopeptidases / Q000235:genetics*; D013381:Subtilisins / Q000235:genetics*
chemical_list: D012697:Serine Endopeptidases; D013381:Subtilisins