Simplified method for preparation of concentrated exoproteins produced by Staphylococcus aureus grown on surface of cellophane bag containing liquid medium.
A simplified method for preparation of concentrated exoproteins including protein A and alpha-toxin produced by Staphylococcus aureus was successfully devised. The concentrated proteins were obtained by cultivating S. aureus organisms on the surface of a liquid medium-containing cellophane bag enclosed in a sterilized glass flask. With the same amount of medium, the total amount of proteins obtained by the method presented here was identical with that obtained by conventional liquid culture. The concentration of proteins obtained by the method, however, was high enough to observe their distinct bands stained on polyacrylamide gel electrophoresis. This method was considered quite useful not only for large-scale cultivation for the purification of staphylococcal proteins but also for small-scale study using the proteins. The precise description of the method was presented and its possible usefulness was discussed.
Ikigai
H
H
Department of Microbiology, Jikei University School of Medicine, Tokyo.
Seki
K
K
Nishihara
S
S
Masuda
S
S
D001427:Bacterial Toxins / Q000096:biosynthesis / Q000302:isolation & purification*; D002476:Cellophane; D003470:Culture Media; D006460:Hemolysin Proteins; D008828:Microbiological Techniques; D013205:Staphylococcal Protein A / Q000096:biosynthesis / Q000302:isolation & purification*; D013211:Staphylococcus aureus / Q000254:growth & development / Q000378:metabolism*
D001427:Bacterial Toxins; D003470:Culture Media; D006460:Hemolysin Proteins; D013205:Staphylococcal Protein A; C021091:staphylococcal alpha-toxin; D002476:Cellophane