[Optimization of methods of immobilization of anti-immunoglobulins. Equilibrium parameters of the interaction of immobilized antibodies with an antigen].
Methods for immobilization of anti-immunoglobulins on insoluble supports were optimized, and the interaction of immunoadsorbents obtained with [125I]-labeled rabbit IgG was investigated. It was shown that this interaction can be adequately described by a rather simple equilibrium model which reflects the interaction of a monovalent antigen with two independent types of binding sites. Within the framework of this model the association constants as well as the concentrations of high affinity binding sites which influence the capacity and efficiency of the separation system were determined. Optimization of the immobilization methods implicated a study on the role of certain functional groups of the antibody involved in the formation of covalent bonds, on the effect of the spacer arm length on the properties of immobilized antibody as well as on the role of the degree of immobilization. It was found that immunoadsorbents obtained after antibody immobilization via lysine or tyrosine residues on matrices with a specific spacer group are the optimal ones.
Guzov
V M
VM
Rodionov
M A
MA
Golubovich
V P
VP
But'kova
L S
LS
Chashchin
V L
VL
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000302:isolation & purification*; D000936:Antigen-Antibody Complex; D000937:Antigen-Antibody Reactions; D001667:Binding, Competitive; D007074:Immunoglobulin G / Q000276:immunology*; D007163:Immunosorbent Techniques; D007164:Immunosorbents; D007700:Kinetics; D010527:Perissodactyla; D011817:Rabbits
D000888:Antibodies, Anti-Idiotypic; D000936:Antigen-Antibody Complex; D007074:Immunoglobulin G; D007164:Immunosorbents
Optimizatsiia metodov immobilizatsii antiimmunoglobulinov. Ravnovesnye parametry vzaimodeĭstviia immobilizirovannykh antitel s antigenom.