Mutation spectrum of the mutagen 3-N,N-acetoxyacetylamino-4,6-dimethyldipyrido[1,2-a:3',2'- d]imidazole in Escherichia coli.
The mutation frequency and mutation spectrum resulting from 3-N-acetylamino-4,6-dimethyldipyrido[1,2-a:3',2'-d]imidazole (AGluP3) DNA adducts using a previously developed forward mutation assay were established. AGluP3-induced mutagenesis requires the umuC gene product(s) and exhibits similar amounts of base pair substitution and frameshift mutation. Comparison between these results and those obtained with the isosteric amine 2-N-acetylaminofluorene suggests the involvement of deacetylated adduct in the molecular mechanisms of AGluP3-induced mutagenesis.
Hebert
E
E
Centre de Biophysique Moléculaire, CNRS, Orleans, France.
Diancourt
F
F
Saint-Ruf
G
G
Leng
M
M
D015073:2-Acetylaminofluorene; D001483:Base Sequence; D004247:DNA / Q000378:metabolism; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics; D007093:Imidazoles / Q000633:toxicity*; D009152:Mutagenicity Tests; D009153:Mutagens
D007093:Imidazoles; D009153:Mutagens; D004247:DNA; C043663:3-(N-acetoxy-N-acetylamino)-4,6-dimethyldipyrido(1,2-a-3',2'-d)imidazole; D015073:2-Acetylaminofluorene