Antibodies against the human cellular 3,3',5-triiodo-L-thyronine-binding protein (p 58).
High-titer antibodies against a cellular thyroid hormone-binding protein (Mr 58,000, p58) were developed by a special immunization method. To enhance immune responses, this method uses a boosting protocol in which repeated injections of small amounts of antigen are administered at 2-day intervals. Antibodies were detected 1 week after the last injection of antigen by ELISA, Western dot blotting and immunoprecipitation. The anti-p58 antibodies recognized p58 which is bound to the thyroid hormone. With the availability of anti-p58 antibodies, it has become possible to study cellular localization and function.
Obata
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T
National Cancer Institute, Laboratory of Molecular Biology, Bethesda, MD 20892.
Fukuda
T
T
Cheng
S Y
SY
D000818:Animals; D000906:Antibodies / Q000032:analysis / Q000276:immunology; D000941:Antigens / Q000008:administration & dosage / Q000276:immunology; D002352:Carrier Proteins / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006433:Hemocyanins / Q000276:immunology; D006801:Humans; D007114:Immunization; D007118:Immunoassay; D007163:Immunosorbent Techniques; D008565:Membrane Proteins / Q000276:immunology*; D051379:Mice; D013963:Thyroid Hormones; D000097810:Thyroid Hormone-Binding Proteins
D000906:Antibodies; D000941:Antigens; D002352:Carrier Proteins; D006433:Hemocyanins; D008565:Membrane Proteins; D013963:Thyroid Hormones; D000097810:Thyroid Hormone-Binding Proteins