Four cDNA clones from the Shaker locus of Drosophila induce kinetically distinct A-type potassium currents in Xenopus oocytes.
The Shaker gene encodes the A-channel of larval and pupal muscle, or one or more of its subunits. Alternative splicing produces messages for several different proteins; two mRNA species have previously been shown to induce the expression of A-currents in Xenopus oocytes. Two additional mRNAs have now been tested and found to produce A-currents in oocytes. The four currents differ in kinetics of inactivation, indicating that the Shaker products may contribute to kinetic diversity in A-channels of the fly and that sequences in both the amino- and carboxy-terminal regions are important for inactivation.
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Howard Hughes Medical Institute, University of California, San Francisco 94143-0724.
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D000818:Animals; D004247:DNA / Q000378:metabolism*; D004330:Drosophila / Q000235:genetics*; D066298:In Vitro Techniques; D008564:Membrane Potentials; D009154:Mutation; D009865:Oocytes / Q000378:metabolism* / Q000502:physiology; D015221:Potassium Channels / Q000378:metabolism* / Q000502:physiology; D014982:Xenopus laevis
D015221:Potassium Channels; D004247:DNA