title: Neuronal influence on glial enzyme expression: evidence from chimeric mouse cerebellum.
abstract: Cerebella, variably deficient in Purkinje cells, were obtained from aggregation chimeras of either Lurcher or Purkinje cell degeneration mutants. These cerebella were used to analyze the expression of glycerol-3-phosphate dehydrogenase (GPDH) in Bergmann glia. Immunocytochemistry showed apparently normal GPDH expression only in Bergmann glia in the immediate vicinity of surviving Purkinje cells. The number of GPDH-positive Bergmann glia cells associated with isolated Purkinje cells was close to that expected, based on measurements in Golgi-stained, normal cerebella of the Bergmann glia cell's domain. The results support the hypothesis that GPDH expression in Bergmann glia cells depends upon their sustained interaction with Purkinje cells, most likely involving direct cell-cell contact.
authors.affiliation: Department of Anatomy and Cell Biology, University of Virginia Medical Center, Charlottesville 22908.
authors.forename: M
authors.initials: M
authors.lastname: Fisher
authors.forename: R J
authors.initials: RJ
authors.lastname: Mullen
mesh_terms: D000818:Animals; D002531:Cerebellum / Q000166:cytology / Q000201:enzymology* / Q000378:metabolism; D002678:Chimera; D015971:Gene Expression Regulation, Enzymologic; D005993:Glycerolphosphate Dehydrogenase / Q000378:metabolism*; D007150:Immunohistochemistry; D051379:Mice; D008809:Mice, Inbred C3H; D008818:Mice, Neurologic Mutants; D009457:Neuroglia / Q000201:enzymology* / Q000378:metabolism
chemical_list: D005993:Glycerolphosphate Dehydrogenase