title: Marker discrepancy as a diagnostic criterion for lymphoid neoplasms.
abstract: Multimarker studies were conducted on 195 lymph node, 59 bone marrow, 44 peripheral blood, eight body fluid, and eight internal organ specimens. The markers were identified by fluorochrome-labeled antibodies quantified with flow cytometry. T-cell receptor gene rearrangements were used for the determination of T-cell clonality. These studies confirmed that CD 19 (B4, Leu 12) is highly sensitive for B-lymphoblastic leukemia, CD 7 (Leu 9) is highly sensitive for T-lymphoblastic leukemia, and CD 5 (Leu 1) is highly sensitive for chronic lymphocytic leukemia. When these markers were compared to antigens of the same cell lineage (e.g., CD 19 to CD 20 [Leu 16] or to surface immunoglobulin, CD 7 to CD 3 [Leu 4], and CD 5 to CD 3), a marked discrepancy between them was diagnostic of the corresponding tumor. T-cell marker discrepancy (CD3 vs. CD 7) was demonstrated in T-cell lymphomas, but it was also shown occasionally in polyclonal T-cell populations. On the other hand, a marked discrepancy between the percentages of a B-lineage (CD 19 or CD 20)-positive and a surface-immunoglobulin-positive population is a reliable phenotype for the diagnosis of a surface-immunoglobulin-negative B-cell lymphoma.
authors.affiliation: Department of Laboratories, Shore University Hospital, Manhasset, New York.
authors.forename: T
authors.initials: T
authors.lastname: Sun
authors.forename: M
authors.initials: M
authors.lastname: Ngu
authors.forename: J
authors.initials: J
authors.lastname: Henshall
authors.forename: J
authors.initials: J
authors.lastname: Cuomo
authors.forename: A
authors.initials: A
authors.lastname: Eisenberg
authors.forename: P
authors.initials: P
authors.lastname: Benn
authors.forename: S L
authors.initials: SL
authors.lastname: Allen
mesh_terms: D000954:Antigens, Surface / Q000032:analysis*; D001402:B-Lymphocytes / Q000276:immunology; D014408:Biomarkers, Tumor; D005434:Flow Cytometry; D015329:Gene Rearrangement, T-Lymphocyte; D006801:Humans; D007945:Leukemia, Lymphoid / Q000175:diagnosis*; D008218:Lymphocytosis / Q000175:diagnosis; D008223:Lymphoma / Q000175:diagnosis*; D010641:Phenotype; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D000954:Antigens, Surface; D014408:Biomarkers, Tumor