Immune carrier properties of acid-treated Salmonella minnesota R595 bacteria. The immune response to TNP-bacterial conjugates in rabbits and mice.
Salmonella minnesota R595 bacteria from which the core region of the lipopolysaccharide on the cell wall had previously been removed by mild acid treatment were trinitrophenylated. Differing amounts of these trinitrophenyl naked bacterial conjugates (TNP-NB), covering a range of epitope densities, were used for immunising mice and rabbits via the intraperitoneal or intravenous routes without adjuvants. It was found that such acid-treated, naked bacteria were effective carriers for the covalently linked hapten, TNP, with an optimum epitope density of 15 micrograms TNP/mg NB. Significant immune responses were obtained with dose levels as low as 50 ng TNP. The possible applications of acid-treated, naked bacteria as universal carriers having inherent adjuvant activity are discussed.
Bellstedt
D U
DU
Max-Planck-Institut für Immunbiologie, Freiburg, F.R.G.
Van der Merwe
K J
KJ
Galanos
C
C
D000085:Acetates; D019342:Acetic Acid; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis*; D000917:Antibody Formation; D002352:Carrier Proteins / Q000008:administration & dosage / Q000276:immunology*; D006386:Hemagglutination Tests; D006461:Hemolysis; D006462:Hemolytic Plaque Technique; D051379:Mice; D009578:Nitrobenzenes / Q000276:immunology*; D011817:Rabbits; D012475:Salmonella / Q000187:drug effects / Q000276:immunology*; D014302:Trinitrobenzenesulfonic Acid / Q000008:administration & dosage / Q000276:immunology*
D000085:Acetates; D000907:Antibodies, Bacterial; D002352:Carrier Proteins; D009578:Nitrobenzenes; D014302:Trinitrobenzenesulfonic Acid; D019342:Acetic Acid