[Specificity of hydrolysis of S. aureus 209P cell walls by a lytic preparation of Pseudomonas lytica].
Specificity of Staphylococcus aureus 209P cell wall hydrolysis by a lytic preparation isolated from the culture liquid filtrate of Pseudomonas lytica VKM V-1454D was studied by the dansylation method. The lytic preparation was found to contain the lytic proteinase lysing the cells of Gram-positive microorganisms. The enzyme hydrolysed the cell walls of S. aureus 209P releasing N-terminal glycine and alanine in amounts of 0.73 and 0.34 mumoles per 1 mumole of lysine, respectively, which indicated the cleavage of the bonds in the pentaglycine bridge and, apparently, of the bond between N-acetylmuramic acid.
Severin
A I
AI
Tauson
E L
EL
Stepnaia
O A
OA
Kulaev
I S
IS
D000596:Amino Acids / Q000378:metabolism; D002473:Cell Wall / Q000378:metabolism; D003619:Dansyl Compounds; D006868:Hydrolysis; D010447:Peptide Hydrolases / Q000378:metabolism*; D010457:Peptidoglycan / Q000378:metabolism; D011549:Pseudomonas / Q000201:enzymology*; D013211:Staphylococcus aureus / Q000378:metabolism*; D013379:Substrate Specificity
D000596:Amino Acids; D003619:Dansyl Compounds; D010457:Peptidoglycan; D010447:Peptide Hydrolases
Issledovanie spetsifichnosti gidroliza kletochnykh stenok S. aureus 209P liticheskim preparatom Pseudomonas lytica.