Sequence and genetic analysis of a dispensible 189 nucleotide snRNA from Saccharomyces cerevisiae.
The structure of a Saccharomyces cerevisiae gene that encodes a small nuclear RNA (snRNA) of 189 nucleotides is described. This gene, designated SNR189, is located 400 base pairs upstream of the CRY1 gene on yeast chromosome III. Gene replacement analysis revealed the SNR189 gene to be dispensable for growth under a variety of culture conditions. The snR189 sequence lacks homology with other sequenced yeast or metazoan snRNAs.
Thompson
J R
JR
Department of Biochemistry, University of Massachusetts, Amherst 01003.
Zagorski
J
J
Woolford
J L
JL
Fournier
M J
MJ
D001483:Base Sequence; D002874:Chromosome Mapping; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012342:RNA, Small Nuclear / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D012342:RNA, Small Nuclear