Minor role of ketone bodies in energy metabolism by skeletal muscle tissue during the postoperative course.
To evaluate changes of peripheral ketone body (KB) metabolism after operation, muscle metabolism in postsurgical patients was studied at 3 hours (SI) and 24 hours (SII) after surgery by the forearm catheter technique. Data were compared to those of equivalent fasted controls (CI, CII). In a manner consistent with enhanced mobilization of endogenous substrate stores, arterial concentrations of free fatty acids (FFA), 3-hydroxybutyrate (3-HOB), and acetoacetate (AcAc) were markedly elevated immediately after surgery. This increase was accompanied by a rise in muscular utilization of AcAc (SI: 0.21 +/- 0.05 mumol/100 g/min; CI: 0.08 +/- 0.05, p less than 0.05) and 3-HOB (SI: 0.24 +/- 0.06 mumol/100 g/min; CI: 0.11 +/- 0.01, p less than 0.05). Surprisingly, on the first postoperative day, concentrations of AcAc and 3-HOB fell below those of fasting controls. Concomitantly, the utilization rate of AcAc by muscle (SII: 0.07 +/- 0.03 mumol/100 g/min; CII: 0.27 +/- 0.04, p less than 0.05) was significantly lower in patients than in controls. Reduction of the fractional extraction rate of AcAc (SI: 38.4 +/- 3.8%; SII: 24.0 +/- 6.1%, p less than 0.05), as well as a net production of 3-HOB by muscle (SII: -0.08 +/- 0.05 mumol/100 g/min; CII: 0.49 +/- 0.13, p less than 0.05) 24 hours after surgery indicated a reduced peripheral capacity for KB removal. Since this finding was related to a significantly higher rate of muscular glycerol production (SII: -0.13 +/- 0.03 mumol/100 g/min; CII: -0.06 +/- 0.02, p less than 0.05), one may suggest that increased intramuscular availability of FFA from triglyceride hydrolysis was responsible for the impairment of peripheral KB utilization. These results indicate that KBs contribute little to energy metabolism in skeletal muscle tissue in the late postoperative phase.
Hartl
W H
WH
Department of Surgery, Klinikum Grosshadern, Munich, West Germany.
Jauch
K W
KW
Kimmig
R
R
Wicklmayr
M
M
Günther
B
B
Heberer
G
G
D020155:3-Hydroxybutyric Acid; D000090:Acetoacetates / Q000097:blood / Q000378:metabolism; D000328:Adult; D001786:Blood Glucose / Q000032:analysis; D004734:Energy Metabolism; D004837:Epinephrine / Q000097:blood; D005215:Fasting; D005230:Fatty Acids, Nonesterified / Q000097:blood / Q000378:metabolism; D005934:Glucagon / Q000097:blood; D005990:Glycerol / Q000096:biosynthesis; D006801:Humans; D006868:Hydrolysis; D006885:Hydroxybutyrates / Q000097:blood / Q000378:metabolism; D007328:Insulin / Q000097:blood; D007657:Ketone Bodies / Q000378:metabolism*; D007773:Lactates / Q000097:blood; D008297:Male; D009132:Muscles / Q000378:metabolism*; D009638:Norepinephrine / Q000097:blood; D010100:Oxygen / Q000097:blood; D011184:Postoperative Period; D013997:Time Factors; D014280:Triglycerides / Q000378:metabolism
D000090:Acetoacetates; D001786:Blood Glucose; D005230:Fatty Acids, Nonesterified; D006885:Hydroxybutyrates; D007328:Insulin; D007657:Ketone Bodies; D007773:Lactates; D014280:Triglycerides; C016635:acetoacetic acid; D005934:Glucagon; D005990:Glycerol; D010100:Oxygen; D020155:3-Hydroxybutyric Acid; D009638:Norepinephrine; D004837:Epinephrine