title: Ancient heat shock gene is dispensable.
abstract: Hsp83 is a major eucaryotic heat shock protein and one of the most conserved proteins known. We have isolated an Escherichia coli gene homologous to eucaryotic Hsp83 and used it to construct a deletion mutation. The E. coli mutant was viable but had a slight growth disadvantage that increased with temperature.
authors.affiliation: Department of Physiological Chemistry, University of Wisconsin Medical School, Madison 53706.
authors.forename: J C
authors.initials: JC
authors.lastname: Bardwell
authors.forename: E A
authors.initials: EA
authors.lastname: Craig
mesh_terms: D002872:Chromosome Deletion; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development; D006360:Heat-Shock Proteins / Q000096:biosynthesis / Q000235:genetics*; D006358:Hot Temperature; D009154:Mutation; D009693:Nucleic Acid Hybridization; D010957:Plasmids
chemical_list: D004269:DNA, Bacterial; D006360:Heat-Shock Proteins