title: Immunoprecipitation of Fasciola hepatica mRNA in vitro translation products using infection and hyperimmune sera.
abstract: Total RNA containing messenger RNA has been isolated from adult Fasciola hepatica and translated in vitro using the rabbit reticulocyte lysate system. L-[35S]-methionine labeled translation products have been immunoprecipitated with sera collected from rabbits infected with F. hepatica, rabbits immunized with adult worm homogenate, and a rabbit and 5 calves immunized with a Fasciola/Schistosoma cross-reactive, cross-protective antigen, denoted FhSmIII(M). Precipitated antigens were analyzed by SDS-polyacrylamide gel electrophoresis. The major antigens recognized by hyperimmune sera had apparent molecular weights of 12,500, 13,500, 14,000, 26,500, and 27,000. The rabbit anti-FhSmIII(M) serum precipitated a 13,500 Mr antigen. Sera collected from calves immunized with FhSmIII(M) also precipitated a 13,500 Mr antigen together with a 26,500 Mr molecule. Antibody with specificity for F. hepatica mRNA translation products could be detected in rabbit serum 2 weeks after infection.
authors.affiliation: Department of Pathology, University of Puerto Rico, San Juan 00936-5067.
authors.forename: G V
authors.initials: GV
authors.lastname: Hillyer
authors.forename: D W
authors.initials: DW
authors.lastname: Taylor
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis; D000947:Antigens, Helminth / Q000235:genetics / Q000276:immunology*; D001345:Autoradiography; D003429:Cross Reactions; D005210:Fasciola hepatica / Q000235:genetics / Q000276:immunology*; D005211:Fascioliasis / Q000276:immunology*; D007106:Immune Sera / Q000276:immunology; D008970:Molecular Weight; D011233:Precipitin Tests; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics*; D011817:Rabbits
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D007106:Immune Sera; D012333:RNA, Messenger