[Application to an automatic system of a direct method for determining atypical variants of cholinesterase using succinyldithiocholine as substrate].
The determination of human serum cholinesterase (EC 3.1.1.8) is frequently requested to detect patients with atypical forms of the enzyme which reacts abnormally with succinylcholine (suxamethonium), employed as a neuromuscular blocking agent. Usually, for biochemical identification of succinylcholine sensitive individuals the standard reaction is run with and without the inhibitors, notably dibucaine. A new test for direct determination of succinylcholine sensitive individuals which use a substrate analogue of succinylcholine was applied to a automated instrument. The linearity, precision, recovery, interference and correlation of the method have been evaluated. We have estimated the reference intervals for a population of 364 healthy subjects subdivided for sex, three atypical homozygotes and two atypical heterozygotes. On the basis of analytical performance we can conclude that this test may offer a further parameter for preoperative screening of individuals with an abnormal response to the muscle relaxant succinylcholine, thus avoiding the determination of genotype by measurement of dibucaine number.
Capone
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Laboratorio di analisi chimico-cliniche e microbiologiche, Ospedale - U.S.L. 25, Bentivoglio, Bologna.
Gnudi
D
D
Pensabeni
L
L
Martelli
A M
AM
Marconi
M C
MC
Accorsi
A
A
D001663:Bilirubin / Q000097:blood; D002802:Cholinesterases / Q000097:blood* / Q000145:classification; D005069:Evaluation Studies as Topic; D005260:Female; D006454:Hemoglobins / Q000032:analysis; D006801:Humans; D008297:Male; D011933:Reagent Kits, Diagnostic; D013390:Succinylcholine
D006454:Hemoglobins; D011933:Reagent Kits, Diagnostic; D002802:Cholinesterases; D013390:Succinylcholine; D001663:Bilirubin
Applicazione a sistema automatico di un metodo diretto per la determinazione delle varianti atipiche della colinesterasi con succinilditiocolina come substrato.