Ethanol inhibits some of the early effects of epidermal growth factor in vivo.
Charles River male Wistar rats (200-300 g) were meal fed for 9-10 days, injected with either saline, epidermal growth factor (EGF), ethanol, or ethanol combined with EGF and their livers were freeze clamped 5 min after intraperitoneal injection of EGF. Metabolites were measured and the redox state and phosphorylation potential were calculated. Epidermal growth factor alone elevated hepatic content of glucose 1-P, glucose 6-P, fructose 6-P, and 3-phosphoglycerate 1.2-1.3-fold when compared to saline treatment. Ethanol alone decreased hepatic content of 3-phosphoglycerate and phosphoenolpyruvate 3.2-3.7-fold below saline-treated levels. Ethanol, in combination with EGF, decreased hepatic values for 3-phosphoglycerate and phosphoenolpyruvate 2.0-2.3-fold from saline treatment but elevated the content of phosphoenolpyruvate 1.6-fold over ethanol treatment alone. Epidermal growth factor inhibited pyruvate kinase activity 1.3-fold when compared to saline controls but ethanol in the presence of EGF facilitated the recovery of activity of this enzyme.
Gerhart
M J
MJ
National Institute on Alcohol Abuse and Alcoholism, Laboratory of Metabolism and Molecular Biology, Rockville, MD 20852.
Reed
B Y
BY
Veech
R L
RL
D000818:Animals; D004734:Energy Metabolism / Q000187:drug effects; D004815:Epidermal Growth Factor / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D000431:Ethanol / Q000494:pharmacology*; D005988:Glyceric Acids / Q000378:metabolism; D006019:Glycolysis / Q000187:drug effects; D007274:Injections, Intraperitoneal; D008099:Liver / Q000187:drug effects; D008297:Male; D010728:Phosphoenolpyruvate / Q000378:metabolism; D011770:Pyruvate Kinase / Q000037:antagonists & inhibitors; D051381:Rats; D011919:Rats, Inbred Strains
D005988:Glyceric Acids; D000431:Ethanol; D004815:Epidermal Growth Factor; D010728:Phosphoenolpyruvate; C005156:3-phosphoglycerate; D011770:Pyruvate Kinase