1H-nuclear magnetic resonance (NMR) spectroscopic study of B lymphocytes stimulated with lipopolysaccharide (LPS).
1H-nuclear magnetic resonance (NMR) spectroscopy was applied to the study of glucose metabolism of B lymphocytes (B cells) activated with lipopolysaccharide (LPS) in a complex medium. The glucose in the medium is degraded to produce lactic acid by B cells activated with LPS to a far larger extent than by non-activated cells.
Ohsaka
A
A
Department of Applied Immunology, National Institute of Health, Tokyo, Japan.
Yoshikawa
K
K
Mizuguchi
J
J
D000409:Alanine / Q000378:metabolism; D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism; D007773:Lactates / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008213:Lymphocyte Activation; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D051379:Mice; D008807:Mice, Inbred BALB C; D011773:Pyruvates / Q000378:metabolism; D013154:Spleen / Q000276:immunology
D007773:Lactates; D008070:Lipopolysaccharides; D011773:Pyruvates; D000431:Ethanol; D000409:Alanine