[Ploidy of a mutant Saccharomyces cerevisiae defective in homologous recombination].
The ploidy of a mutant of Saccharomyces cerevisiae defective in recombination (Rec-) has been determined using tetrad analysis and flowing fluorometry. Evidence is obtained that the effect of the Rec- phenotype, i.e. the increase of the stability of plasmids with 2 mkm DNA ori replication in the yeast cirO cells is not the result of the diploidy in cells of the Rec- mutant developed in the process of transformation.
Semenova
V D
VD
Arman
I P
IP
D003434:Crossing Over, Genetic; D004271:DNA, Fungal / Q000032:analysis; D005434:Flow Cytometry; D005800:Genes, Fungal; D005821:Genetic Techniques; D009154:Mutation; D010641:Phenotype; D011003:Ploidies; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014170:Transformation, Genetic
D004271:DNA, Fungal
Ploidnost' mutanta Saccharomyces cerevisiae, defektnogo po gomologichnoĭ rekombinatsii.