A general procedure for screening inhibitory antibodies: application for identifying anti-protein kinase C antibodies.
In this communication we describe a microfiltration assay to identify monoclonal antibodies that interfere with the activity of enzymes. This method is quick and sensitive to small changes in the activity of the enzyme and does not require highly purified enzyme or large quantities of antibodies. It has been applied to identify anti-protein kinase C antibodies which would have been impossible to identify by classical assays such as enzyme-linked immunosorbent assay.
Mochly-Rosen
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Department of Biochemistry, University of California, Berkeley 94720.
Koshland
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D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D002848:Chromatography, DEAE-Cellulose; D005374:Filtration; D007158:Immunologic Techniques; D008832:Microchemistry; D011493:Protein Kinase C / Q000276:immunology*
D000911:Antibodies, Monoclonal; D011493:Protein Kinase C