Syngeneic male graft rejection by B6 female mice primed with spleen and testes of Sxr and Sxr' mice.
Presence or absence of the mammalian male-specific H-Y transplantation antigen was investigated in Sxr and Sxr' mice. Groups of C57BL/6 (B6) female mice were primed with spleen cells or testicular homogenates of such mice and were subsequently grafted with syngeneic male skin. Median graft survival times (MSTs) were compared with the MSTs of control groups: (i) unprimed B6 females, (ii) B6 females primed with B6 female spleen, and, (iii) B6 females primed with B6 male spleen or testes. The MSTs of the groups primed with B6 male and Sxr spleen were significantly shorter than, and those of the groups primed with B6 female and Sxr' spleen similar to the MST of unprimed females. The MSTs of groups of B6 female mice primed with testicular homogenates of B6, Sxr, and Sxr' males did not differ from the MST of unprimed B6 females. Taken together, this indicates that spleen of B6, and Sxr males carry the H-Y transplantation antigen, while the antigen is lacking on spleen cells of B6 females and Sxr' males. The results of priming with testis are interpreted such that (i) either do testes of B6, Sxr and Sxr' males lack the antigen, or, (ii) priming with testes homogenates renders B6 females unresponsive to H-Y of male grafts.
Wiberg
U H
UH
Institut für Humangenetik und Anthropologie, Freiburg, BRD.
Lattermann
U
U
D000818:Animals; D005260:Female; D006084:Graft Rejection / Q000276:immunology; D006182:H-Y Antigen / Q000032:analysis*; D008297:Male; D051379:Mice; D008817:Mice, Mutant Strains; D012732:Sex Determination Analysis; D016038:Skin Transplantation / Q000276:immunology; D013154:Spleen / Q000276:immunology / Q000637:transplantation; D013737:Testis / Q000276:immunology* / Q000637:transplantation
D006182:H-Y Antigen