title: Protein synthesis in polyamine-deficient bacteria during amino-acid starvation.
abstract: Amino-acid starvation in polyamine-auxotrophic bacteria grown in the presence of putrescine provokes a marked inhibition of protein synthesis. This inhibition is almost completely relieved in polyamine-depleted cells. The differential behaviour of bacterial protein synthesis depending on the endogenous levels of polyamines is not due to a change in the uptake of amino acids used to measure protein synthesis, nor to the decreased growth rate of polyamine-depleted cells. During leucine starvation, cells grown with putrescine synthesized a somewhat lower amount of high-molecular-weight proteins than polyamine-depleted bacteria. In addition, cells with normal endogenous levels of polyamines accumulated significant amounts of 62 and 41 kDa polypeptides as well as several low-molecular-weight peptides.
authors.affiliation: Instituto de Investigaciones Bioquímicas Fundacion Campomar, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina.
authors.forename: H G
authors.initials: HG
authors.lastname: Nastri
authors.forename: I D
authors.initials: ID
authors.lastname: Algranati
mesh_terms: D000596:Amino Acids / Q000378:metabolism*; D001426:Bacterial Proteins / Q000096:biosynthesis*; D001692:Biological Transport / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D007700:Kinetics; D008970:Molecular Weight; D011073:Polyamines / Q000378:metabolism*; D011700:Putrescine / Q000494:pharmacology*
chemical_list: D000596:Amino Acids; D001426:Bacterial Proteins; D011073:Polyamines; D011700:Putrescine