title: A soluble enzyme activity that attaches free diaminopimelic acid to bdelloplast peptidoglycan.
abstract: An enzyme activity, responsible for the attachment of diaminopimelic acid (DAP) to bdelloplast wall peptidoglycan, was studied in an in vitro, cell-free system. Most of the activity was found in the high-speed (20000g) supernatant fraction of homogenates of bdelloplasts prepared from a culture of the intracellular bacterium Bdellovibrio bacteriovorus 109J, growing synchronously within cells of Escherichia coli. Peptidoglycan preparations obtained either from E. coli ML35 or from the walls of bdelloplasts synchronously cultured for 40 or 90 min served as the acceptors in this reaction, whereas cell wall or peptidoglycan preparations obtained from Gram-positive bacteria could not function as acceptors of DAP. The attachment activity had an apparent Km value for DAP of 10 microM; for bdelloplast peptidoglycan, it was approximately 0.43 mg/mL, which is 13 microM with respect to peptidoglycan disaccharide peptide units. DAP attachment was partially inhibited by the structural analogues lanthionine, L-ornithine, beta-aminobutyric acid, and D-serine, as well as the cell wall synthesis inhibitors penicillin G, ampicillin, and cephalexin. This enzyme activity is present only during the intracellular stage of the bdellovibrio's developmental growth cycle and may serve a stage-specific function of biochemically modifying the cell in which it grows.
authors.affiliation: Department of Biological Sciences, University of Southern California, Los Angeles 90089-0371.
authors.forename: Y
authors.initials: Y
authors.lastname: Araki
authors.forename: E G
authors.initials: EG
authors.lastname: Ruby
mesh_terms: D000596:Amino Acids / Q000494:pharmacology; D000599:Amino Acids, Diamino / Q000378:metabolism*; D000667:Ampicillin / Q000494:pharmacology; D001501:Bdellovibrio / Q000201:enzymology*; D002473:Cell Wall / Q000187:drug effects; D002506:Cephalexin / Q000494:pharmacology; D003960:Diaminopimelic Acid / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology; D007700:Kinetics; D010400:Penicillin G / Q000494:pharmacology; D010457:Peptidoglycan / Q000378:metabolism*
chemical_list: D000596:Amino Acids; D000599:Amino Acids, Diamino; D010457:Peptidoglycan; D003960:Diaminopimelic Acid; D000667:Ampicillin; D002506:Cephalexin; D010400:Penicillin G