title: Processing by inverted plasma membrane vesicles of in vitro synthesized major lipoprotein from Escherichia coli.
abstract: Synthesis, lipid modification and proteolytic processing of the major lipoprotein from Escherichia coli is shown to occur in a homologous in vitro transcription-translation system containing inverted plasma membrane vesicles. The primary translation product (cross-reacting with anti-lipoprotein antiserum) is a precursor which is converted into a lower molecular mass species of the size of mature lipoprotein by the addition of inverted membrane vesicles from E. coli. Conversion is prevented by globomycin, a specific inhibitor of the unique lipoprotein-signal peptidase II, which is active only on lipid-containing precursors. The inverted plasma membrane vesicles used here must therefore contain active lipid-modifying enzymes and signal peptidase II.
authors.affiliation: Institute of Biochemistry, University of Freiburg, FRG.
authors.forename: S S
authors.initials: SS
authors.lastname: Krishnabhakdi
authors.forename: M
authors.initials: M
authors.lastname: Müller
mesh_terms: D002462:Cell Membrane / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D008074:Lipoproteins / Q000096:biosynthesis / Q000235:genetics*; D014176:Protein Biosynthesis; D011499:Protein Processing, Post-Translational; D014158:Transcription, Genetic
chemical_list: D008074:Lipoproteins