Interactions of triiodothyronine, insulin and dexamethasone on the binding of human LDL to rat hepatocytes in monolayer culture.
Rat hepatocytes were maintained for the first 24 h in culture in the presence of 10% (v/v) newborn calf serum and then for a further 16 h in serum-free medium containing 2 g bovine serum albumin per litre. The presence of 1-100 nM triiodothyronine (T3) in the second incubation significantly increased binding of human 125I-LDL to the LDL receptor. Unlike insulin, T3 was unable to reverse the decrease in binding brought about by dexamethasone. The increased binding to the LDL receptor produced by insulin and T3 was additive. We conclude that T3, insulin and glucocorticoids may play important roles in regulating plasma LDL concentrations by direct effect on LDL uptake by the liver.
Salter
A M
AM
Department of Biochemistry, University of Nottingham Medical School, Queen's Medical Centre, U.K.
Fisher
S C
SC
Brindley
D N
DN
D000818:Animals; D002478:Cells, Cultured; D003907:Dexamethasone / Q000494:pharmacology*; D004347:Drug Interactions; D006801:Humans; D066298:In Vitro Techniques; D007328:Insulin / Q000494:pharmacology*; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D051381:Rats; D011973:Receptors, LDL / Q000187:drug effects; D014284:Triiodothyronine / Q000494:pharmacology*
D007328:Insulin; D008077:Lipoproteins, LDL; D011973:Receptors, LDL; D014284:Triiodothyronine; D003907:Dexamethasone