title: Measurement of rat insulin. Enzyme-linked immunosorbent assay with increased sensitivity, high accuracy, and greater practicability than established radioimmunoassay.
abstract: Total immunoreactive insulin (IRI) is conventionally determined by radioimmunoassays. IRI measurement in rats can be made more sensitive, accurate, and practical, as demonstrated by a new modified enzyme-linked immunosorbent assay (ELISA). It is characterized by indirect binding of an anti-insulin antibody by an antiglobulin antibody and uses the principle of competitive saturation. In this ELISA, IRI can be determined in a wide range of concentrations, corresponding to the standards. The standard curve ranges from 100 to 0.049 ng/ml IRI (1 ng/ml approximately 23.4 microU/ml approximately 172 pM rat insulin). The statistical analysis shows between- and within-assay coefficients of variation of less than or equal to 15%.
authors.affiliation: Department of Internal Medicine, University of Kiel, Federal Republic of Germany.
authors.forename: J
authors.initials: J
authors.lastname: Kekow
authors.forename: K
authors.initials: K
authors.lastname: Ulrichs
authors.forename: W
authors.initials: W
authors.lastname: Müller-Ruchholtz
authors.forename: W L
authors.initials: WL
authors.lastname: Gross
mesh_terms: D000818:Animals; D002021:Buffers; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D007202:Indicators and Reagents; D007328:Insulin / Q000097:blood*; D011863:Radioimmunoassay; D051381:Rats; D012015:Reference Standards; D013223:Statistics as Topic
chemical_list: D002021:Buffers; D007202:Indicators and Reagents; D007328:Insulin