title: [Use of immunoenzyme analysis on nitrocellulose membranes for assessing different methods of purifying the yellow mosaic virus of beans].
abstract: The comparative study of different methods for the purification of bean yellow mosaic virus isolated from lupine has been made. To evaluate the method of purification, electron-microscopic, spectrophotometric and ELISA techniques have been used. The possibility of using ELISA on nitrocellulose filters with the utilization of a peroxidase-antiperoxidase complex, manufactured in the USSR, for the determination of viral carriership in plants has been shown.
authors.forename: O V
authors.initials: OV
authors.lastname: Molchanets
authors.forename: N V
authors.initials: NV
authors.lastname: Taĭkova
authors.forename: L I
authors.initials: LI
authors.lastname: Zhmurko
authors.forename: T B
authors.initials: TB
authors.lastname: Grashchenko
authors.forename: A L
authors.initials: AL
authors.lastname: Boĭko
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D000956:Antigens, Viral / Q000032:analysis; D003101:Collodion; D007887:Fabaceae / Q000382:microbiology; D007124:Immunoenzyme Techniques / Q000295:instrumentation; D008567:Membranes, Artificial; D008854:Microscopy, Electron; D009029:Mosaic Viruses / Q000276:immunology / Q000302:isolation & purification*; D010946:Plants, Medicinal; D011817:Rabbits; D012639:Seeds / Q000382:microbiology; D012692:Serial Passage; D013053:Spectrophotometry
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D008567:Membranes, Artificial; D003101:Collodion
vernacular_title: Ispol'zovanie immunofermentnogo analiza na nitrotselliuloznykh membranakh dlia otsenki razlichnykh sposobov ochistki virusa zheltoĭ mozaiki fasoli.