[Semiautomatic procedure for determining urine iron in subjects treated with deferoxamine B].
A semiautomatic method for the determination of urine iron during treatment with deferoxamine B has been evaluated: samples were diluted 1:10 with a TCA 10% and thioglycolic acid 1.1% solution and processed after centrifugation by the aca III discrete clinical analyzer Du Pont. In this way an overestimation of values depending on the turbidity caused by proteins is avoided and the bad smell is limited thanks to the very low concentration of the thioglycolic acid solution. A good correlation with the atomic absorption spectrophotometry, specially for values less than or equal to 20 mg/l, was observed examining urine samples of 150 patients treated with deferoxamine B.
Grisler
R
R
Laboratorio di ricerche cliniche, anatomia ed istologia patologica, Istituti clinici di perfezionamento, Milano.
Giavardi
C
C
Agape
V
V
Scaricabarozzi
R
R
Guercilena
S
S
D003676:Deferoxamine / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D007501:Iron / Q000378:metabolism / Q000652:urine*; D008722:Methods; D013864:Thioglycolates
D013864:Thioglycolates; C017487:2-mercaptoacetate; D007501:Iron; D003676:Deferoxamine
Procedimento semiautomatico per la determinazione del ferro urinario in soggetti trattati con deferossamina B.