Identification and isolation of core histones from Schizosaccharomyces pombe.
Core histones have been isolated from Schizosaccharomyces pombe and compared electrophoretically to core histones from Saccharomyces cerevisiae and rat liver. The molecular masses of all cognate histones examined were found to be very similar as determined by SDS gel electrophoresis. Histones H3, H2A and H2B from Sch. pombe migrated almost identically to their respective counterparts from S. cerevisiae as determined by acid/urea gel electrophoresis. Two-dimensional gel electrophoresis with a Triton X-100 acid/urea gel in the first dimension followed by an SDS gel in the second dimension was used to separate Sch. pombe histones from contaminating ribosomal proteins.
Whiteside
S G
SG
Department of Biology, Boston College, Chestnut Hill, MA 02167.
Plocke
D J
DJ
D000818:Animals; D002467:Cell Nucleus / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D006657:Histones / Q000302:isolation & purification*; D008099:Liver / Q000032:analysis*; D008970:Molecular Weight; D051381:Rats; D012269:Ribosomal Proteins / Q000302:isolation & purification*; D012270:Ribosomes / Q000032:analysis; D012441:Saccharomyces cerevisiae / Q000032:analysis; D004718:Saccharomycetales / Q000032:analysis*; D012568:Schizosaccharomyces / Q000032:analysis*
D006657:Histones; D012269:Ribosomal Proteins