title: Genotypic analysis using a Y-chromosome-specific probe following bone marrow transplantation.
abstract: To monitor successful engraftment after bone marrow transplantation, we performed Southern hybridization analysis or dot blot analysis of DNA in a set of sex-mismatched cases using a Y-chromosome-specific DNA probe (pHY10). This method was extremely sensitive and rapid for checking which cells contain the Y-chromosome. Using this probe, analysis of cells from peripheral blood and bone marrow after transplantation demonstrated the usefulness of confirming engraftment of donor cells and of detecting mixed lymphohematopoietic chimerism.
authors.affiliation: Department of Pathological Pharmacology, University of Tokyo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Morisaki
authors.forename: T
authors.initials: T
authors.lastname: Morisaki
authors.forename: Y
authors.initials: Y
authors.lastname: Nakahori
authors.forename: H
authors.initials: H
authors.lastname: Ogura
authors.forename: H
authors.initials: H
authors.lastname: Kanno
authors.forename: K
authors.initials: K
authors.lastname: Tani
authors.forename: H
authors.initials: H
authors.lastname: Kodo
authors.forename: H
authors.initials: H
authors.lastname: Fujii
authors.forename: S
authors.initials: S
authors.lastname: Asano
authors.forename: S
authors.initials: S
authors.lastname: Miwa
mesh_terms: D000328:Adult; D001853:Bone Marrow / Q000032:analysis; D016026:Bone Marrow Transplantation; D004247:DNA / Q000032:analysis; D005260:Female; D005838:Genotype; D006801:Humans; D007951:Leukemia, Myeloid / Q000097:blood / Q000235:genetics / Q000628:therapy; D015470:Leukemia, Myeloid, Acute / Q000097:blood / Q000235:genetics / Q000628:therapy; D008297:Male; D009693:Nucleic Acid Hybridization; D014998:Y Chromosome
chemical_list: D004247:DNA