title: The inhibition of skeletal changes in a rat burn model with a local muscle flap.
abstract: The effects of early wound closure using a local muscle flap on the development of periosteal new bone formation in a rat burn model were studied. Following a full-thickness burn to one hind limb, periosteal new bone formation along the tibial diaphysis was measured by the use of the fluorochrome agent calcein and an image-analysis system. Prostaglandin E levels, a known inflammatory mediator, from the bone beneath the burn also were measured. Periosteal new bone formation was inhibited by 50 percent in animals that had debridement and wound closure with a gastrocnemius muscle flap and skin graft on postburn day 2 compared to untreated controls or animals closed with skin grafts only. There was a trend toward reduced prostaglandin E measurements from tibial sections in the early closure group compared to untreated controls. This study demonstrates that early wound closure using a local muscle flap inhibits the periosteal new bone formation which is possibly associated with the inflammation in a rat burn model.
authors.affiliation: Shriners Burns Institute, University of Cincinnati College of Medicine, Ohio.
authors.forename: J D
authors.initials: JD
authors.lastname: Wyrick
authors.forename: P J
authors.initials: PJ
authors.lastname: Stern
authors.forename: L P
authors.initials: LP
authors.lastname: Bruno
authors.forename: J W
authors.initials: JW
authors.lastname: Alexander
mesh_terms: D000818:Animals; D001846:Bone Development; D002056:Burns / Q000601:surgery*; D003646:Debridement; D004195:Disease Models, Animal; D005260:Female; D005452:Fluoresceins; D010521:Periosteum / Q000502:physiology; D011458:Prostaglandins E / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D016038:Skin Transplantation; D013524:Surgical Flaps; D013977:Tibia / Q000502:physiology
chemical_list: D005452:Fluoresceins; D011458:Prostaglandins E; C007740:fluorexon