A quantitative analysis of 2-D gels identifies proteins in which labeling is increased following long-term sensitization in Aplysia.
Long-term memory for sensitization of the gill- and siphon-withdrawal reflex in Aplysia, produced by 4 days of training, is associated with increased synaptic efficacy of the connection between the sensory and motor neurons. This training is also accompanied by neuronal growth; there is an increase in the number of synaptic varicosities per sensory neuron and in the number of active zones. Such structural changes may be due to changes in the rates of synthesis of certain proteins. We have searched for proteins in which the rates of [35S]methionine labeling are altered during the maintenance phase of long-term memory for sensitization by using computer-assisted quantitative 2-D gel analysis. This method has allowed us to detect 4 proteins in which labeling is altered after 4 days of sensitization training.
Castellucci
V F
VF
Center for Neurobiology and Behavior, College of Physicians and Surgeons, Columbia University, New York, New York.
Kennedy
T E
TE
Kandel
E R
ER
Goelet
P
P
D000818:Animals; D001048:Aplysia; D001345:Autoradiography; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D005724:Ganglia / Q000378:metabolism*; D008568:Memory; D008715:Methionine / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000302:isolation & purification; D012018:Reflex; D012984:Software; D013462:Sulfur Radioisotopes
D009419:Nerve Tissue Proteins; D013462:Sulfur Radioisotopes; D008715:Methionine