Impairment of human polymorphonuclear leukocyte chemotaxis by 2,5-hexanedione.
The effect of n-hexane metabolites on human polymorphonuclear leukocyte chemotaxis and luminol-dependent chemiluminescence was investigated. No effect was detected when 2-hexanol, 2-hexanone and gamma-valerolactone were used; 2,5-hexanedione at 75 micrograms/ml inhibited chemotaxis and a direct correlation between increasing the xenobiotic concentration and the degree of inhibition was found. Chemotactic peptide-induced chemiluminescence was not affected by 2,5-hexanedione. In order to clarify the phenomenon, plasma membrane fluidity was investigated by fluorescence polarization of the fluorescent probe trimethylammonium diphenylhexatriene. 2,5-hexanedione increased the membrane fluidity, while the other n-hexane metabolites did not change the degree of fluorescence polarization. Results suggest that the cellular functions modulated by membrane-cytoskeletal organization are affected by 2,5-hexanedione also at the low concentrations.
Governa
M
M
Institute of Occupational Medicine, University of Ancona, Italy.
Valentino
M
M
Visona
I
I
Rocco
M
M
D002633:Chemotaxis / Q000187:drug effects*; D006588:Hexanones / Q000494:pharmacology*; D006801:Humans; D007659:Ketones / Q000494:pharmacology*; D008560:Membrane Fluidity / Q000187:drug effects*; D009504:Neutrophils / Q000187:drug effects*
D006588:Hexanones; D007659:Ketones; C011269:2,5-hexanedione