title: Cell specificity of nuclear protein antigens in the development of Xenopus species.
abstract: Protein antigens accumulated in the oocyte nucleus of Xenopus spp. are found later in all nuclei of the embryo. During and after organogenesis, the fate of different nuclear antigens diverges: Some are permanently expressed in all nuclei, some are no longer detectable in any nuclei, and some show a high degree of specificity relative to cell type. Of the class of antigens permanently expressed in all cells, a nucleolar antigen has been studied that is relatively enriched in the gonia of both sexes. A possible correlations of its occurrence with ribosomal DNA amplification and transcription is examined. The cell-specific expression of 4 antigens has been analyzed in detail, using indirect immunofluorescent staining methods as well as conventional histology. The tissue-specificity of 2 of these antigens does not change during metamorphosis, whereas the pattern of others is dynamic until after metamorphosis. We conclude that the observed tissue-specificity does not correlate with descendence from one of the 3 primary germ layers: Some antigens appear to accumulate in the nucleus when a cell takes up its specific function.
authors.affiliation: Max-Planck-Institut für Entwicklungsbiologie, Abteilung für Zellbiologie, Tübingen, Federal Republic of Germany.
authors.forename: D
authors.initials: D
authors.lastname: Wedlich
authors.forename: C
authors.initials: C
authors.lastname: Dreyer
mesh_terms: D000375:Aging; D000818:Animals; D002466:Cell Nucleolus / Q000648:ultrastructure; D004247:DNA / Q000032:analysis; D004625:Embryo, Nonmammalian / Q000502:physiology* / Q000648:ultrastructure; D005260:Female; D005455:Fluorescent Antibody Technique; D008297:Male; D009687:Nuclear Proteins / Q000032:analysis* / Q000276:immunology; D009693:Nucleic Acid Hybridization; D009928:Organ Specificity; D014316:Tritium; D014981:Xenopus / Q000196:embryology / Q000254:growth & development*; D014982:Xenopus laevis / Q000196:embryology / Q000254:growth & development*
chemical_list: D009687:Nuclear Proteins; D014316:Tritium; D004247:DNA