Hexadecane-induced skin hyperplasia in the hairless rat: time course of histological and biochemical events related to the synthesis of polyamines and DNA.
Several biochemical parameters including ornithine decarboxylase activity (ODC) and tissue polyamine levels were measured during the hexadecane-induced epidermal hyperplasia of hairless rat skin. Animals received three applications of 200 microliters pure n-hexadecane on day 1. ODC activity and polyamine levels (putrescine, spermidine and spermine) in the epidermis were significantly increased and reached maximum elevations at 12 h after the start of n-hexadecane treatment with DNA synthesis peaking at 24 h. Histological studies confirmed a significant cellular edema at 24 h after the beginning of the treatment followed at 48 h by an epidermal hyperplasia which was maximum at 72 h. These data support the view that ODC activation, increased biosynthesis of polyamines and DNA are early events in epidermal cell hyperproliferation.
Bouclier
M
M
Centre International de Recherches Dermatologiques (CIRD), Sophia Antipolis, Valbonne, France.
Shroot
B
B
Milano
G
G
Ferracin
J
J
Chatelus
A
A
Hensby
C N
CN
D000473:Alkanes / Q000633:toxicity*; D000818:Animals; D015317:Biogenic Polyamines / Q000096:biosynthesis*; D004247:DNA / Q000096:biosynthesis*; D006965:Hyperplasia; D008297:Male; D009955:Ornithine Decarboxylase / Q000378:metabolism; D051381:Rats; D012867:Skin / Q000378:metabolism / Q000473:pathology*; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors
D000473:Alkanes; D015317:Biogenic Polyamines; D004247:DNA; D009955:Ornithine Decarboxylase; C007932:n-hexadecane; D013936:Thymidine