Two-dimensional electrophoresis computerized processing.
This paper describes various methods suitable for implementation of two-dimensional processing software. The different steps leading to a complete processing are described, from the digitalization of the image to the processing of the resulting data. The characteristics of a convenient digitalization system are discussed. The different software devoted to spot detection is reviewed with respect to the presence or otherwise of a spot model and its characteristics. The major techniques for gel matching are compared as are designs for database structures suitable for tabulation of measurements. Finally, the need for a sophisticated system of data processing is stressed and its main requirements are described.
Vincens
P
P
Ecole Normale Supérieure UA 686 CNRS, Paris, France.
Tarroux
P
P
D003201:Computers; D004586:Electrophoresis / Q000379:methods*; D010455:Peptides / Q000302:isolation & purification*; D011506:Proteins / Q000302:isolation & purification*
D010455:Peptides; D011506:Proteins