ICAM-1 a ligand for LFA-1-dependent adhesion of B, T and myeloid cells.
Cell-cell adhesion is essential for many immunological functions. The LFA-1 molecule, a member of a superfamily of adhesion molecules, participates in adhesion which is critical to the function of each of the three major subsets of leukocytes: lymphocytes, monocytes and granulocytes. Putative LFA-1 ligands have been identified functionally in different laboratories using three different monoclonal antibodies that inhibit LFA-1-mediated leukocyte adhesion in particular model systems; however, there may be more than one LFA-1 ligand. We have directly compared the three relevant monoclonal antibodies, and show that each binds to the same molecule, intercellular-adhesion molecule-1 (ICAM-1). Most important, B, T and myeloid cells adhere specifically to purified ICAM-1-coated surfaces; such adhesion has distinctive requirements for Mg2+ and Ca2+. This constitutes biochemical evidence that ICAM-1 functions as a ligand for LFA-1-dependent adhesion by a variety of leukocytes.
Makgoba
M W
MW
Immunology Branch, NCI, Bethesda, Maryland 20892.
Sanders
M E
ME
Ginther Luce
G E
GE
Dustin
M L
ML
Springer
T A
TA
Clark
E A
EA
Mannoni
P
P
Shaw
S
S
D000911:Antibodies, Monoclonal / Q000276:immunology; D000954:Antigens, Surface / Q000276:immunology / Q000502:physiology*; D001402:B-Lymphocytes / Q000502:physiology*; D002118:Calcium / Q000502:physiology; D002448:Cell Adhesion; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D016169:Lymphocyte Function-Associated Antigen-1; D008274:Magnesium / Q000502:physiology; D013602:T-Lymphocytes, Cytotoxic / Q000502:physiology*; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D016169:Lymphocyte Function-Associated Antigen-1; D008274:Magnesium; D002118:Calcium