Synovial fluid neutrophils of patients with rheumatoid arthritis have membrane antigen changes that reflect activation.
The level of expression of surface antigens was studied on neutrophils from paired samples of blood and synovial fluid of patients with rheumatoid arthritis (RA). By measuring the immunofluorescence of labelled monoclonal antibodies it was shown that on joint neutrophils there was an increase in expression of complement receptor 3 (CR3) and granulocyte functional antigens 1 and 2 compared with blood neutrophils, whereas the expression of GpIIb-IIIa was reduced. The pattern of expression was the same as that seen with in vitro activation and strongly suggest that activation is occurring within the joint.
Emery
P
P
Reid Memorial Laboratory of the Clinical Research Unit, Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, South Australia.
Lopez
A F
AF
Burns
G F
GF
Vadas
M A
MA
D000954:Antigens, Surface / Q000276:immunology*; D001172:Arthritis, Rheumatoid / Q000097:blood / Q000276:immunology*; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007249:Inflammation / Q000276:immunology; D039841:Leukocyte L1 Antigen Complex; D016177:Macrophage-1 Antigen; D008297:Male; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000276:immunology*; D010980:Platelet Membrane Glycoproteins / Q000276:immunology; D011951:Receptors, Complement / Q000276:immunology; D013582:Synovial Fluid / Q000166:cytology / Q000276:immunology*
D000954:Antigens, Surface; D039841:Leukocyte L1 Antigen Complex; D016177:Macrophage-1 Antigen; D010980:Platelet Membrane Glycoproteins; D011951:Receptors, Complement; D009240:N-Formylmethionine Leucyl-Phenylalanine