title: Purification to homogeneity and some properties of squalene synthetase.
abstract: Squalene synthetase has been purified to homogeneity from yeast. It is a single polypeptide of Mr 47,000. This enzyme catalyzes the synthesis of squalene from farnesyl diphosphate via presqualene diphosphate. In the presence of reduced pyridine nucleotides, presqualene diphosphate and squalene are produced in a ratio of 6:1 from either the purified protein or the crude microsomal fraction.
authors.affiliation: Department of Biochemistry, University of Utah School of Medicine, Salt Lake City 84108.
authors.forename: K
authors.initials: K
authors.lastname: Sasiak
authors.forename: H C
authors.initials: HC
authors.lastname: Rilling
mesh_terms: D002845:Chromatography; D004591:Electrophoresis, Polyacrylamide Gel; D013186:Farnesyl-Diphosphate Farnesyltransferase / Q000302:isolation & purification* / Q000378:metabolism; D007525:Isoelectric Focusing; D008970:Molecular Weight; D009243:NAD / Q000494:pharmacology; D009249:NADP / Q000494:pharmacology; D010088:Oxidoreductases / Q000302:isolation & purification*; D011106:Polyisoprenyl Phosphates / Q000096:biosynthesis / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D012717:Sesquiterpenes; D013185:Squalene / Q000096:biosynthesis
chemical_list: D011106:Polyisoprenyl Phosphates; D012717:Sesquiterpenes; D009243:NAD; C017672:presqualene pyrophosphate; D009249:NADP; C004808:farnesyl pyrophosphate; D013185:Squalene; D010088:Oxidoreductases; D013186:Farnesyl-Diphosphate Farnesyltransferase