Correlation between classification of human urothelial cell lines and HLA-A,B,C expression.
Quantitative changes in major histocompatibility class I antigen expression in tumour cells are believed to affect the host immune response against the tumour. In tumourigenic (TGrIII) human urothelial cell lines the apparent loss of polymorphic HLA-A,B epitopes has previously been demonstrated. In the present study, 3 non-tumourigenic (TGrII) and 6 tumourigenic (TGrIII) human urothelial cell lines have been investigated for their quantitative expression of monomorphic HLA-A,B,C and B2-microglobulin. Evidence is provided that an inverse correlation exists between tumourigenicity and HLA-A,B,C and B2-microglobulin expression. Furthermore, treatment of the cells with neuraminidase partly restored the expression of monomorphic HLA-A,B,C suggesting that at least some of the observed quantitative differences could be due to masking of the membrane bound HLA antigens by sialic acid-containing glycoconjugates.
Ottesen
S S
SS
Fibiger Institute, Danish Cancer Society, Copenhagen, Denmark.
Tromholt
V
V
Kieler
J
J
D000951:Antigens, Neoplasm / Q000276:immunology; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000276:immunology*; D004847:Epithelial Cells; D004848:Epithelium / Q000276:immunology; D006680:HLA Antigens / Q000032:analysis*; D006801:Humans; D001743:Urinary Bladder / Q000166:cytology* / Q000276:immunology; D001613:beta 2-Microglobulin / Q000032:analysis*
D000951:Antigens, Neoplasm; D006680:HLA Antigens; D001613:beta 2-Microglobulin