Detection of HLA antibodies by platelet crossmatching techniques.
Thirty-seven monospecific HLA antibodies directed against all common HLA-A and -B loci and reactive by the microlymphocytotoxicity assay (LCT) were tested against platelets carrying the corresponding antigen by three platelet crossmatch methods, the platelet immunofluorescence test (PIFT), platelet enzyme-linked immunosorbent assay (P-ELISA), and platelet radioimmunoassay (P-RIA). Positive reactions were obtained with the PIFT in 67 percent, the P-ELISA in 41 percent, and the P-RIA in 49 percent of 85 cell-serum pairs. The same cell-serum combinations gave 49 percent positive reactions in the lymphocyte immunofluorescence test. Three multispecific HLA antisera were positive in nine of nine cell-serum combinations by all four methods. Thirteen transfusions were given to eight patients with known HLA antibodies. All donor-recipient pairs were LCT positive, six were PIFT positive, and seven were PIFT negative. Three of seven PIFT-negative and none of six PIFT-positive transfusions were successful. Thus, platelet crossmatching is less sensitive than the LCT for the detection of complement-binding monospecific HLA antibodies. The platelet crossmatch, however, is able to identify some potentially successful HLA-incompatible donors for patients with multispecific HLA antibodies and limited access to HLA-identical donors.
McGrath
K
K
Red Cross Blood Bank, Alfred Hospital, Melbourne, Australia.
Holdsworth
R
R
Veale
M
M
Bishop
J
J
Wolf
M
M
D000906:Antibodies / Q000032:analysis*; D001788:Blood Grouping and Crossmatching / Q000379:methods; D001792:Blood Platelets; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006680:HLA Antigens / Q000276:immunology*; D006801:Humans; D008722:Methods; D011863:Radioimmunoassay
D000906:Antibodies; D006680:HLA Antigens