Recombinant human interleukin 6 (B-cell stimulatory factor 2) is a potent inducer of differentiation of mouse myeloid leukemia cells (M1).
Recombinant human interleukin 6 (IL-6), a lymphokine involved in the final differentiation of activated B-cells into antibody-forming cells, greatly suppressed proliferation and induced differentiation of murine myeloid leukemia cells (M1) into mature macrophage-like cells. When M1 cells were treated with IL-6, their growth was completely arrested as early as on day 2, and they were induced to differentiate morphologically into macrophage-like cells. Differentiation-associated properties such as phagocytic activity, adherence to the dish surface, Fc and C3 receptors, were also induced within 24 h by IL-6, and they reached their respective maximal levels on day 2 or 3. The potency of IL-6 in suppressing proliferation and inducing differentiation was much greater than that of 1 alpha,25-dihydroxyvitamin D3 one of the most potent inducers of M1 cells. The present report indicates that IL-6 is involved in the differentiation of not only B-cells but also myeloid leukemia cells.
Miyaura
C
C
Department of Biochemistry, School of Dentistry, Showa University, Tokyo, Japan.
Onozaki
K
K
Akiyama
Y
Y
Taniyama
T
T
Hirano
T
T
Kishimoto
T
T
Suda
T
T
D000818:Animals; D002117:Calcitriol / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D006801:Humans; D015850:Interleukin-6; D007378:Interleukins / Q000494:pharmacology*; D007951:Leukemia, Myeloid / Q000473:pathology*; D016177:Macrophage-1 Antigen; D008264:Macrophages / Q000473:pathology; D051379:Mice; D010587:Phagocytosis / Q000187:drug effects; D011951:Receptors, Complement / Q000096:biosynthesis / Q000187:drug effects; D011961:Receptors, Fc / Q000096:biosynthesis / Q000187:drug effects; D014407:Tumor Cells, Cultured
D015850:Interleukin-6; D007378:Interleukins; D016177:Macrophage-1 Antigen; D011951:Receptors, Complement; D011961:Receptors, Fc; D002117:Calcitriol