title: Lymphatic filariasis: detection of circulating and urinary antigen and differences in antibody isotypes complexed with circulating antigen between symptomatic and asymptomatic subjects.
abstract: A two-site immunoradiometric assay using a monoclonal antibody (MoAb) against Brugia malayi microfilariae allowed the detection of parasite molecules both in the serum and the urine of patients from Sri Lanka infected with Wuchereria bancrofti. Whereas 50% of patients had no antigen in their serum, all of them excreted detectable amounts of antigen in their urine, the levels being higher in symptomatic than in asymptomatic patients. The poor detection in serum appeared to be related to the presence of circulating immune complexes. It was shown that the isotype of the antibodies complexed with the circulating antigen was IgM in the asymptomatic group, while it was mainly IgG in the symptomatic patients (swelling and lymphoedema or elephantiasis). These results suggest the existence of regulatory immune mechanisms affecting the clinical expression of lymphatic filariasis.
authors.affiliation: Centre d'Immunologie et de Biologie Parasitaire, Unité Mixte INSERM 167-CNRS 624, Institut Pasteur, Lille, France.
authors.forename: C
authors.initials: C
authors.lastname: Lutsch
authors.forename: J Y
authors.initials: JY
authors.lastname: Cesbron
authors.forename: D
authors.initials: D
authors.lastname: Henry
authors.forename: J P
authors.initials: JP
authors.lastname: Dessaint
authors.forename: K
authors.initials: K
authors.lastname: Wandji
authors.forename: M
authors.initials: M
authors.lastname: Ismail
authors.forename: A
authors.initials: A
authors.lastname: Capron
mesh_terms: D000818:Animals; D000936:Antigen-Antibody Complex / Q000032:analysis; D000947:Antigens, Helminth / Q000652:urine*; D002009:Brugia / Q000276:immunology; D004605:Elephantiasis, Filarial / Q000276:immunology*; D005368:Filariasis / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D007132:Immunoglobulin Isotypes / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology; D008842:Microfilariae / Q000276:immunology; D011863:Radioimmunoassay; D014958:Wuchereria bancrofti / Q000276:immunology
chemical_list: D000936:Antigen-Antibody Complex; D000947:Antigens, Helminth; D007074:Immunoglobulin G; D007132:Immunoglobulin Isotypes; D007075:Immunoglobulin M