Cellular distribution and amount of chromogranin A in bovine endocrine pancreas.
We determined the cellular distribution and the amount of chromogranin A in endocrine cells of bovine pancreas using a polyclonal antibody against bovine adrenomedullary chromogranin A. The relative amounts of chromogranin A in the different cells of the endocrine pancreas were determined by computer-assisted analyses of the optical densities of the immunoreactivities in the stained sections. More than 80% of the immunoreactive chromogranin A was located in the pancreatic B-cells. In immunoblots of acid tissue extracts, only one chromogranin A band (MW 74 KD) was observed. Quantification of the immunoblots revealed that 3 micrograms of chromogranin A and 918 micrograms of insulin were present per gram pancreas (wet weight), equivalent to a molar ratio of 460 mumol chromogranin A per mol insulin.
Ehrhart
M
M
Abteilung Anatomie und Zellbiologie, Universität Ulm, Bundesrepublik Deutschland.
Jörns
A
A
Grube
D
D
Gratzl
M
M
D000818:Animals; D002417:Cattle; D053379:Chromogranin A; D002864:Chromogranins / Q000032:analysis*; D007150:Immunohistochemistry; D007328:Insulin / Q000032:analysis; D007515:Islets of Langerhans / Q000032:analysis* / Q000166:cytology; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000032:analysis*; D012016:Reference Values
D053379:Chromogranin A; D002864:Chromogranins; D007328:Insulin; D009419:Nerve Tissue Proteins