title: Immunohistochemical study on the endocrine pancreas of cattle with special reference to coexistence of serotonin and glucagon or bovine pancreatic polypeptide.
abstract: Bovine pancreatic endocrine cells were investigated by light microscopic immunohistochemistry. Serotonin-immunoreactive cells as well as insulin-, glucagon-, somatostatin-, bovine pancreatic polypeptide (BPP)-immunoreactive cells were detected in the pancreatic islets. Generally, insulin-immunoreactive cells were distributed throughout the islet and the others took peripheral location. Since the distribution and shape of serotonin-immunoreactive cells were very similar to glucagon- and BPP-immunoreactive cells, serial sections were restained by using the elution method. All glucagon- and BPP-immunoreactive cells also showed serotonin immunoreactivity but glucagon and BPP immunoreactivities were never observed to be colocalized in the same cell. A small number of serotonin-immunoreactive cells were observed that showed serotonin immunoreactivity only.
authors.affiliation: Department of Veterinary Anatomy, Obihiro University of Agriculture and Veterinary Medicine, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Nakajima
authors.forename: N
authors.initials: N
authors.lastname: Kitamura
authors.forename: J
authors.initials: J
authors.lastname: Yamada
authors.forename: T
authors.initials: T
authors.lastname: Yamashita
authors.forename: T
authors.initials: T
authors.lastname: Watanabe
mesh_terms: D000818:Animals; D002417:Cattle; D005260:Female; D005934:Glucagon / Q000032:analysis*; D007150:Immunohistochemistry; D007515:Islets of Langerhans / Q000032:analysis* / Q000166:cytology / Q000276:immunology; D010187:Pancreatic Hormones / Q000032:analysis; D010191:Pancreatic Polypeptide / Q000032:analysis* / Q000276:immunology; D012701:Serotonin / Q000032:analysis* / Q000276:immunology
chemical_list: D010187:Pancreatic Hormones; D012701:Serotonin; D010191:Pancreatic Polypeptide; D005934:Glucagon