Interleukin 1 induces CD1 antigen expression on human gingival epithelial cells.
The CD1 (T6) antigen is a highly specific marker for human Langerhans cells (LC). Previous studies have demonstrated that crude preparations containing murine interleukin-1 (IL-1) or a human epithelial cell-derived IL-1 inhibitor (ILS) modulate CD1 expression by LC in organ culture. This study examined the effect of organ-culture derived human IL-1, recombinant human IL-1, and purified ILS on CD1 expression in dispersed epithelial cell cultures. Both IL-1 preparations stimulated CD1 expression in whole and CD1-depleted cultures. The optimal dose level for this effect was 0.5 U/ml. Higher dose levels did not result in an increase in CD1 expression, implying that a limited pool of CD1 negative EC are induced to express CD1 by IL-1. Induction of CD1 expression on whole and depleted EC was abrogated by ILS. These results indicate that human IL-1 and an IL-1 inhibitor act in combination to modulate CD1 expression on Langerhans cells in the gingival epithelium.
Walsh
L J
LJ
Department of Social and Preventive Dentistry, University of Queensland Dental School, Brisbane, Australia.
Seymour
G J
GJ
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis*; D002478:Cells, Cultured; D004848:Epithelium / Q000276:immunology; D005881:Gingiva / Q000276:immunology*; D006801:Humans; D007124:Immunoenzyme Techniques; D007375:Interleukin-1 / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D007801:Langerhans Cells / Q000166:cytology / Q000276:immunology; D008222:Lymphokines / Q000494:pharmacology; D011994:Recombinant Proteins
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D007375:Interleukin-1; D008222:Lymphokines; D011994:Recombinant Proteins