Cloning and expression of DNA coding for the major house dust mite allergen Der p 1 in Escherichia coli.
A cDNA clone coding for the major house dust mite allergen Der p 1 was isolated from a lambda gt 11 library. Its sequence correlates with known amino acid sequences of Der p 1 and it produces a fusion protein which reacts with rabbit anti-Der p 1 antiserum.
Thomas
W R
WR
Clinical Immunology Research Unit, Princess Margaret Hospital, Subiaco, Australia.
Stewart
G A
GA
Simpson
R J
RJ
Chua
K Y
KY
Plozza
T M
TM
Dilworth
R J
RJ
Nisbet
A
A
Turner
K J
KJ
D000485:Allergens / Q000235:genetics / Q000302:isolation & purification*; D000595:Amino Acid Sequence; D000818:Animals; D039741:Antigens, Dermatophagoides; D001483:Base Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D008925:Mites / Q000235:genetics* / Q000276:immunology; D008969:Molecular Sequence Data
D000485:Allergens; D039741:Antigens, Dermatophagoides