Molecular structure, conformation and interactions of antitumor antibiotic cyanonaphthridinomycin, a covalent binder of DNA.
X-ray, NMR and molecular modeling studies on cyanonaphthridinomycin (C22H26N4O5), a DNA binding antibiotic, have been carried out to study the structure, conformation and interactions with DNA. The crystals belong to the space group P21 with the cell dimensions of a = 5.934(1)b = 20.684(4), c = 16.866(3)A, gamma = 90.9 degrees and Z = 4(two molecules/asymmetric unit). The structure was solved by direct methods and difference Fourier methods and refined to an R value of 0.087 for 4061 reflections. The conformation of the molecule is compared with that of naphthridinomycin. There are differences in the orientation of the methoxyl group and the saturated oxazole ring. 1 and 2D NMR studies have been carried out and the dihedral angles obtained from coupling constants have been compared with those obtained from the crystal structure. Molecular mechanics studies were carried out to obtain the energy minimized structure and its comparison with X-ray and NMR results. Molecular modelling studies were performed to propose models for drug-DNA interactions. Both partial intercalation and groove-binding models have been proposed.
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Drug Dynamics Institute Medicinal Chemistry Division College of Pharmacy, University of Texas Austin 78712.
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D000903:Antibiotics, Antineoplastic; D003198:Computer Simulation; D004247:DNA; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D009287:Naphthyridines; D009690:Nucleic Acid Conformation
D000903:Antibiotics, Antineoplastic; D009287:Naphthyridines; C035070:cyanonaphthyridinomycin; D004247:DNA