title: Proton NMR studies of the GDP.Mg2+ complex of the Ha-ras oncogene product p21.
abstract: Two-dimensional proton NMR studies were performed on the c-Ha-ras encoded proto-oncogene product p21C. COSY and NOESY spectra of the p21C.GDP.Mg2+ complex show that the ribose H1 proton of the bound GDP is in close proximity to the aromatic side chain of a phenylalanyl residue. From sequence homology with the bacterial elongation factor Tu (EF-Tu) and the known X-ray structure of the EF-Tu.GDP.Mg2+ complex it may be inferred that the Phe residue in question is either Phe78 or Phe82 in the p21 sequence.
authors.affiliation: Max-Planck-Institut für medizinische Forschung, Abteilung für Biophysik, Heidelberg, FRG.
authors.forename: I
authors.initials: I
authors.lastname: Schlichting
authors.forename: A
authors.initials: A
authors.lastname: Wittinghofer
authors.forename: P
authors.initials: P
authors.lastname: Rösch
mesh_terms: D006150:Guanine Nucleotides / Q000378:metabolism*; D006153:Guanosine Diphosphate / Q000378:metabolism*; D008274:Magnesium / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D016283:Proto-Oncogene Proteins p21(ras)
chemical_list: D006150:Guanine Nucleotides; D011518:Proto-Oncogene Proteins; D006153:Guanosine Diphosphate; D016283:Proto-Oncogene Proteins p21(ras); D008274:Magnesium