Proton NMR studies of the GDP.Mg2+ complex of the Ha-ras oncogene product p21.
Two-dimensional proton NMR studies were performed on the c-Ha-ras encoded proto-oncogene product p21C. COSY and NOESY spectra of the p21C.GDP.Mg2+ complex show that the ribose H1 proton of the bound GDP is in close proximity to the aromatic side chain of a phenylalanyl residue. From sequence homology with the bacterial elongation factor Tu (EF-Tu) and the known X-ray structure of the EF-Tu.GDP.Mg2+ complex it may be inferred that the Phe residue in question is either Phe78 or Phe82 in the p21 sequence.
Schlichting
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Max-Planck-Institut für medizinische Forschung, Abteilung für Biophysik, Heidelberg, FRG.
Wittinghofer
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Rösch
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D006150:Guanine Nucleotides / Q000378:metabolism*; D006153:Guanosine Diphosphate / Q000378:metabolism*; D008274:Magnesium / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D016283:Proto-Oncogene Proteins p21(ras)
D006150:Guanine Nucleotides; D011518:Proto-Oncogene Proteins; D006153:Guanosine Diphosphate; D016283:Proto-Oncogene Proteins p21(ras); D008274:Magnesium