Binding of mercuric chloride to nuclear proteins in human peripheral blood T lymphocytes.
The binding of labelled (203Hg) mercuric chloride to nuclear proteins of human peripheral blood T lymphocytes was studied by using polyacrylamide gel electrophoresis and autoradiography. Most of the nuclear proteins were found to be able to bind mercuric chloride; however, 4 of the proteins were heavily labelled. Of these 4 proteins, 2 migrated in the histone region, 1 comigrating exactly with histone H3. The other 2 proteins were non-histone proteins with molecular weight of approximately 25 and 43 kD.
Holst
M
M
Department of Medical Cell Biology, Karolinska Institutet, Stockholm, Sweden.
Nordlind
K
K
D000328:Adult; D001345:Autoradiography; D001665:Binding Sites; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008627:Mercuric Chloride / Q000378:metabolism*; D008970:Molecular Weight; D009687:Nuclear Proteins / Q000032:analysis / Q000378:metabolism*; D013601:T-Lymphocytes / Q000378:metabolism*
D009687:Nuclear Proteins; D008627:Mercuric Chloride