Site-specific oligonucleotide binding represses transcription of the human c-myc gene in vitro.
A 27-base-long DNA oligonucleotide was designed that binds to duplex DNA at a single site within the 5' end of the human c-myc gene, 115 base pairs upstream from the transcription origin P1. On the basis of the physical properties of its bound complex, it was concluded that the oligonucleotide forms a colinear triplex with the duplex binding site. By means of an in vitro assay system, it was possible to show a correlation between triplex formation at -115 base pairs and repression of c-myc transcription. The possibility is discussed that triplex formation (site-specific RNA binding to a DNA duplex) could serve as the basis for an alternative program of gene control in vivo.
Cooney
M
M
Department of Molecular Biology, Princeton University, NJ 08544.
Czernuszewicz
G
G
Postel
E H
EH
Flint
S J
SJ
Hogan
M E
ME
D004586:Electrophoresis; D005786:Gene Expression Regulation; D006801:Humans; D066298:In Vitro Techniques; D009693:Nucleic Acid Hybridization; D009838:Oligodeoxyribonucleotides / Q000494:pharmacology*; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D011519:Proto-Oncogenes; D014158:Transcription, Genetic / Q000187:drug effects
D009838:Oligodeoxyribonucleotides; D011518:Proto-Oncogene Proteins