title: Relationship between the induction of mitotic gene conversion and the formation of thymine glycols in yeast S. cerevisiae treated with hydrogen peroxide.
abstract: The effects of hydrogen peroxide on yeast Saccharomyces cerevisiae were assessed by measuring gene conversion at the trp 5 locus and the amount of thymine glycols in DNA using a monoclonal antibody specific to this base modification. Our results show that: (a) hydrogen peroxide-induced mitotic gene conversion in yeast strain D7M1 was dose-dependent in the low dose range where no toxicity was observed; (b) in the low dose range, the frequency of gene conversion depended on the temperature of the treatment, with more conversion at 25 degrees C than at 15 degrees C; (c) thymine glycols were induced in DNA in a dose-dependent manner following exposure of cells to up to 400 mM hydrogen peroxide; (d) there was little difference in the amount of thymine glycols formed in DNA when treatment occurred at either 25 degrees C or 15 degrees C.
authors.affiliation: Biophysics Division, National Cancer Center Research Institute, Tokyo, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Kaneko
authors.forename: S A
authors.initials: SA
authors.lastname: Leadon
authors.forename: T
authors.initials: T
authors.lastname: Ito
mesh_terms: D000911:Antibodies, Monoclonal; D004305:Dose-Response Relationship, Drug; D005785:Gene Conversion / Q000187:drug effects*; D006861:Hydrogen Peroxide / Q000633:toxicity*; D008938:Mitosis / Q000187:drug effects; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics; D013941:Thymine / Q000031:analogs & derivatives* / Q000276:immunology; D014364:Tryptophan
chemical_list: D000911:Antibodies, Monoclonal; C029389:thymine glycol; D014364:Tryptophan; D006861:Hydrogen Peroxide; D013941:Thymine