title: Sequencing and overexpression of the Escherichia coli aroE gene encoding shikimate dehydrogenase.
abstract: The Escherichia coli aroE gene encoding shikimate dehydrogenase was sequenced. The deduced amino acid sequence was confirmed by N-terminal amino acid sequencing and amino acid analysis of the overproduced protein. The complete polypeptide chain has 272 amino acid residues and has a calculated Mr of 29,380. E. coli shikimate dehydrogenase is homologous to the shikimate dehydrogenase domain of the fungal arom multifunctional enzymes and to the catabolic quinate dehydrogenase of Neurospora crassa.
authors.affiliation: Department of Biochemistry, University of Glasgow, Scotland, U.K.
authors.forename: I A
authors.initials: IA
authors.lastname: Anton
authors.forename: J R
authors.initials: JR
authors.lastname: Coggins
mesh_terms: D000244:Adenosine Diphosphate / Q000378:metabolism; D000429:Alcohol Oxidoreductases / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D001483:Base Sequence; D003062:Codon; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D005786:Gene Expression Regulation; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D013045:Species Specificity
chemical_list: D003062:Codon; D000244:Adenosine Diphosphate; D000429:Alcohol Oxidoreductases; C022641:Shikimate dehydrogenase