title: The abnormal cerebellar organization of Weaver and reeler mice does not affect the cellular distribution of three neuronal mRNAs.
abstract: We used in situ hybridization of 35S-labeled antisense RNAs to study the cellular distribution of three neuronal mRNAs. We compared the expression of these RNAs in cerebellar Purkinje neurons in wild-type (C57Bl-6J) mice and in two mutants (Weaver and reeler) known to have abnormal cerebellar morphologies. In normal mice, GAD mRNA is present in four sets of neurons in the cerebellar cortex while calbindin mRNA is present only in Purkinje neurons. Proenkephalin mRNA is present in Golgi II neurons as well as in a set of neurons in the deep part of the molecular layer. Despite the dramatic differences in structural organization and inputs of Purkinje neurons in the cerebella of adult Weaver and reeler mice, the expression of these RNAs appears unchanged. These results support the hypothesis that Purkinje cell cytodifferentiation proceeds autonomously after its inception in early embryonic life.
authors.affiliation: Department of Biology, University of California, Los Angeles 90024.
authors.forename: C W
authors.initials: CW
authors.lastname: Wuenschell
authors.forename: A J
authors.initials: AJ
authors.lastname: Tobin
mesh_terms: D000818:Animals; D064026:Calbindins; D002531:Cerebellum / Q000166:cytology / Q000378:metabolism* / Q000473:pathology; D004745:Enkephalins / Q000378:metabolism*; D005786:Gene Expression Regulation; D005968:Glutamate Decarboxylase / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D008818:Mice, Neurologic Mutants / Q000033:anatomy & histology / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D011498:Protein Precursors / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D064030:S100 Calcium Binding Protein G / Q000378:metabolism*
chemical_list: D064026:Calbindins; D004745:Enkephalins; D011498:Protein Precursors; D012333:RNA, Messenger; D064030:S100 Calcium Binding Protein G; C029992:proenkephalin; D005968:Glutamate Decarboxylase