Intracellular picrotoxin blocks pentobarbital-gated Cl- conductance.
Using the 'inside-out' configuration of frog sensory neurons, we studied the effect of intracellular picrotoxin on the pentobarbital-gated single channel response of Cl- -current (iCl). The pentobarbital-induced iCl showed no voltage-dependency and the single channel conductance (gamma Cl) was 16 +/- 3.1 pS (n = 6). Picrotoxin caused the pentobarbital-gated Cl- channels to react in a flickering pattern and then finally caused them to cease their opening altogether. This inhibitory action of picrotoxin was reversible.
Inomata
N
N
Department of Physiology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Tokutomi
N
N
Oyama
Y
Y
Akaike
N
N
D000818:Animals; D002712:Chlorides / Q000502:physiology*; D005727:Ganglia, Spinal / Q000187:drug effects / Q000502:physiology*; D066298:In Vitro Techniques; D008564:Membrane Potentials / Q000187:drug effects; D008845:Microinjections; D010424:Pentobarbital / Q000494:pharmacology*; D010852:Picrotoxin / Q000494:pharmacology*; D011892:Rana catesbeiana
D002712:Chlorides; D010424:Pentobarbital; D010852:Picrotoxin