A pre-embedding immunolabeling technique for basal lamina and extracellular matrix molecules.
We have developed a pre-embedding immunolabeling technique to identify basal lamina and extracellular matrix molecules in embryos at various stages of development. The technique works for both fluorescence optical microscopy (1-2.5-micron sections) and for transmission electron microscopy, and enables straigthforward correlation between the two. An additional advantage is the easy preparation of well-oriented serial sections, facilitating detailed studies of development.
Martins-Green
M M
MM
Department of Zoology, University of California, Davis 95616.
Tokuyasu
K T
KT
D000818:Animals; D001485:Basement Membrane / Q000032:analysis* / Q000196:embryology; D002642:Chick Embryo; D003094:Collagen / Q000032:analysis; D005109:Extracellular Matrix / Q000032:analysis*; D005353:Fibronectins / Q000032:analysis; D005455:Fluorescent Antibody Technique; D006046:Gold; D007150:Immunohistochemistry / Q000379:methods*; D007797:Laminin / Q000032:analysis; D051379:Mice; D008854:Microscopy, Electron; D013205:Staphylococcal Protein A
D005353:Fibronectins; D007797:Laminin; D013205:Staphylococcal Protein A; D006046:Gold; D003094:Collagen