Expression and processing of a recombinant human macrophage colony-stimulating factor in mouse cells.
A human macrophage colony-stimulating factor encoded by a 4-kilobase cDNA was expressed with bovine papillomavirus vectors in mouse cells. Pulse-chase analyses revealed that the 62-kilodalton (kDa) translation product was glycosylated, cleaved, and efficiently secreted within 1 h of synthesis. The secreted product contained both N-linked and O-linked oligosaccharide chains. Macrophage colony-stimulating factor was present extracellularly as an 80-kDa homodimer and as a multimeric species of greater than 200 kDa that may be associated with other glycoproteins.
Manos
M M
MM
Department of Molecular Biology, Cetus Corporation, Emeryville, California 94608.
D000818:Animals; D003115:Colony-Stimulating Factors / Q000235:genetics / Q000378:metabolism*; D004247:DNA / Q000235:genetics; D005822:Genetic Vectors; D006031:Glycosylation; D006801:Humans; D007700:Kinetics; D051379:Mice; D011499:Protein Processing, Post-Translational
D003115:Colony-Stimulating Factors; D004247:DNA