Selective cytotoxic activity of immunotoxins composed of a monoclonal anti-Thy 1.1 antibody and the ribosome-inactivating proteins bryodin and momordin.
The ribosome-inactivating proteins, bryodin, from Bryonia dioica, and momordin, from Momordica charantia, were coupled by a disulphide bond to a monoclonal anti-Thy 1.1 antibody (OX7). Both immunotoxins were specifically cytotoxic to the Thy 1.1-expressing mouse lymphoma cell line AKR-A in vitro. The OX7-bryodin immunotoxins were the more powerfully toxic and reduced protein synthesis in AKR-A cells by 50% at a concentration of 1-4 x 10(-11) M as compared with 1 x 10(-9) M for the OX7-momordin immunotoxins. Neither of the immunotoxins was toxic to mouse lymphoma EL4 cells, which lack the Thy 1.1 antigen, at concentrations up to 3 x 10(-8) M. Further, bryodin and momordin immunotoxins made from an antibody (R10) of irrelevant specificity were without effect on AKR-A cells.
Stirpe
F
F
Dipartimento di Patologia Sperimentale, Universita degli Studi di Bologna, Italy.
Wawrzynczak
E J
EJ
Brown
A N
AN
Knyba
R E
RE
Watson
G J
GJ
Barbieri
L
L
Thorpe
P E
PE
D000818:Animals; D000911:Antibodies, Monoclonal / Q000627:therapeutic use; D000964:Antimetabolites, Antineoplastic / Q000627:therapeutic use; D000922:Immunotoxins / Q000627:therapeutic use*; D007518:Isoantibodies; D008223:Lymphoma / Q000188:drug therapy; D051379:Mice; D009699:N-Glycosyl Hydrolases; D010940:Plant Proteins / Q000627:therapeutic use*; D054789:Ribosome Inactivating Proteins, Type 1; D054790:Ribosome Inactivating Proteins, Type 2; D013601:T-Lymphocytes / Q000276:immunology; D014118:Toxins, Biological; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000911:Antibodies, Monoclonal; D000964:Antimetabolites, Antineoplastic; D000922:Immunotoxins; D007518:Isoantibodies; D010940:Plant Proteins; D054789:Ribosome Inactivating Proteins, Type 1; D054790:Ribosome Inactivating Proteins, Type 2; D014118:Toxins, Biological; C035678:anti-Thy antibody; C051829:bryodin; D009699:N-Glycosyl Hydrolases