Blood cyclosporine concentrations in liver transplant recipients: assay method and influence of changed hepatic and renal function.
In 154 blood samples from adult and pediatric liver transplant recipients with variable degrees of hepatic and renal dysfunction, the specific monoclonal antibody-based Sandimmune RIA kit gave CsA results that were highly correlated with HPLC values. In contrast, assay of CsA levels with the PARIA in 213 samples showed values that varied between 1.8 and 18.8 times those determined by HPLC. In adults, the extent of this variation in the CsA HPLC/PARIA ratio was highly correlated with values of BIL and to a lesser extent with serum ALP; additionally, in children, correlations with AST and GGT were noted.
Tredger
J M
JM
Liver Unit, King's College Hospital, London, England.
Steward
C M
CM
Williams
R
R
D000328:Adult; D002648:Child; D002851:Chromatography, High Pressure Liquid; D003524:Cyclosporins / Q000097:blood*; D006801:Humans; D007668:Kidney / Q000502:physiology; D008099:Liver / Q000502:physiology; D016031:Liver Transplantation; D011863:Radioimmunoassay
D003524:Cyclosporins