title: [Determining the level of the rheumatoid factor associated with polyclonal and monoclonal human serum IgA].
abstract: The authors studied the activity of the rheumatoid factor (RF) associated with polyclonal and monoclonal IgA contained in the sera of patients with chronic diseases of noninfectious etiology and A-paraproteinemia. IgA-RF-activity was determined by an enzyme immunoassay. In most of the patients' sera IgA had a high level of RF-activity. The frequency of RF-activity detection did not depend on the level of polyclonal IgA but correlated with the level of monoclonal serum IgA. During a study of the chromatographic fractions of sera containing monoclonal IgA, RF-activity was mainly revealed in the fraction of IgA polymers; IgA monomers forming the bulk of serum IgA, demonstrated no RF-activity. The inclination of curves reflecting the correlation between the level of IgA-RF and dilutions of tested sera made it possible to assess function of the affinity of such molecules which was growing with an increase in IgA molecular mass.
authors.forename: G P
authors.initials: GP
authors.lastname: German
authors.forename: E Iu
authors.initials: EIu
authors.lastname: Varlamova
authors.forename: N E
authors.initials: NE
authors.lastname: Andreeva
mesh_terms: D000911:Antibodies, Monoclonal / Q000032:analysis*; D000915:Antibody Affinity; D002850:Chromatography, Gel; D002908:Chronic Disease; D005922:Glomerulonephritis, IGA / Q000276:immunology*; D006801:Humans; D007124:Immunoenzyme Techniques; D007070:Immunoglobulin A / Q000032:analysis*; D010265:Paraproteinemias / Q000276:immunology*; D012217:Rheumatoid Factor / Q000032:analysis*
chemical_list: D000911:Antibodies, Monoclonal; D007070:Immunoglobulin A; D012217:Rheumatoid Factor
vernacular_title: Opredelenie revmatoidnogo faktora, sviazannogo s poliklonal'nym i monoklonal'nym syvorotochnym IgA cheloveka.