title: Human muscle glutathione S-transferase (GST-4) shows close homology to human liver GST-1.
abstract: Human muscle specific glutathione S-transferase (RX: glutathione R-transferase, EC 2.5.1.18) (GST-4) and liver GST-1 have been purified and subjected to N-terminal sequence analysis. These two isozymes show close homology and only differ in 3 residues within the first 24. The N-terminal sequences of GST-1 and GST-4 differ significantly from those of GST-2 and GST-3. Although antiserum raised against native GST-1 did not cross-react with GST-4, cross-reactivity was obtained with antiserum raised against denatured GST-1. The homology between GST-1 and GST-4 indicates that they are both members of the mu evolutionary class.
authors.affiliation: Department of Human Genetics, John Curtin School of Medical Research, Australian National University, Canberra.
authors.forename: P G
authors.initials: PG
authors.lastname: Board
authors.forename: T
authors.initials: T
authors.lastname: Suzuki
authors.forename: D C
authors.initials: DC
authors.lastname: Shaw
mesh_terms: D000595:Amino Acid Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D005982:Glutathione Transferase; D006801:Humans; D007124:Immunoenzyme Techniques; D007527:Isoenzymes; D008099:Liver / Q000201:enzymology*; D008969:Molecular Sequence Data; D009132:Muscles / Q000201:enzymology*; D012689:Sequence Homology, Nucleic Acid
chemical_list: D007527:Isoenzymes; D005982:Glutathione Transferase