[Epidermal growth factor with insulin used for stimulation of exogenous colony formation in the spleen of mice irradiated with lethal doses of X-rays].
Epidermal growth factor (12 ng per 1 g of body mass) and insulin (0.004 units per 1 g of body mass) were introduced into X-ray irradiated (1.8, 2.12, 2.7 Cr) mice. Four hours later bone marrow was extracted from femurs to be introduced into syngenic lethally irradiated recipients. On the 11th day after transplantation the number of exogenic spleen colonies was computed. The epidermal growth factor, in combination with insulin, stimulates in the organism the restoration of hemopoietic colony-forming cells after radiation injury.
Gracheva
N I
NI
Zhestianikov
V D
VD
Bil'din
V N
VN
Pereverzev
A E
AE
Sorokin
A B
AB
D000818:Animals; D016026:Bone Marrow Transplantation; D003114:Colony-Forming Units Assay; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D006412:Hematopoietic Stem Cells / Q000187:drug effects* / Q000528:radiation effects; D007328:Insulin / Q000494:pharmacology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D013154:Spleen / Q000187:drug effects* / Q000528:radiation effects; D013268:Stimulation, Chemical; D013997:Time Factors; D014185:Transplantation, Isogeneic
D007328:Insulin; D004815:Epidermal Growth Factor
Stimuliatsiia épidermal'nym faktorom rosta sovmestno s insulinom obrazovaniia ékzogennykh koloniĭ v selezenke mysheĭ, letal'no obluchennykh rentgenovskimi luchami.