Structural studies of glucorhamnans isolated from the lipopolysaccharides of reference strains for Serratia marcescens serogroups O4 and O7, and of an O14 strain.
Partially acetylated glucorhamnans have been isolated from the lipopolysaccharides of three strains of Serratia marcescens. The polymer from the reference strain (C.D.C. 864-57) for serogroup O4 has the disaccharide repeating-unit shown below, in which acetylation at position 2 of the rhamnosyl residue is approximately 90% complete. Similar glucorhamnans from the reference strain (C.D.C. 843-57) for serogroup O7 and from a pigmented strain (NM) of serogroup O14 differ only in the configuration of the L-rhamnopyranosyl residue (beta) and the extent of O-acetylation (O7, almost stoichiometric; NM, 80-90%). Glucorhamnans of the second type have been isolated previously from the lipopolysaccharides of other strains of S. marcescens, including the reference strain for serogroup O6 and another pigmented O14 strain (N.C.T.C. 1377). In all cases, the lipopolysaccharide extracts also contained acidic glycans, but the glucorhamnans are believed to constitute the integral side-chains. (Formula: see text).
Oxley
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Department of Chemistry, The University, Hull, Great Britain.
Wilkinson
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SG
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D008070:Lipopolysaccharides; D009682:Magnetic Resonance Spectroscopy; D011135:Polysaccharides, Bacterial / Q000302:isolation & purification*; D012210:Rhamnose / Q000302:isolation & purification; D012706:Serratia marcescens / Q000276:immunology*
D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial; D012210:Rhamnose