The complex of poly(dG).poly(dC) with arginine: stabilization of the B form and transition to multistranded structures.
We have studied by X-ray diffraction fibers of complexes of poly(dG).poly(dC) with N-alpha-acetyl-L-arginine ethylamide. Although these polynucleotides favour the A form of DNA, in this complex it is never found, thus confirming that arginine prevents the appearance of this form of DNA. At high relative humidity the B form is present. Upon dehydration two new structures appear. One of them is a triple helix, most likely formed by poly(dC+).poly(dG).poly(dC). The other structure found also has features which indicate a multistranded conformation.
Campos
J L
JL
Unidad Quimica Macromolecular del C.S.I.C. Escuela T.S. de Ingenieros Industriales, Barcelona, Spain.
Subirana
J A
JA
D001120:Arginine / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D004247:DNA / Q000648:ultrastructure; D009690:Nucleic Acid Conformation / Q000187:drug effects; D011089:Polydeoxyribonucleotides / Q000378:metabolism*
D011089:Polydeoxyribonucleotides; C016681:poly(dG).poly(dC); C062944:N-alpha-acetylarginine-ethylamide; D004247:DNA; D001120:Arginine