Oncogene expression in adenocarcinomas of the colon and in colon tumor-derived cell lines.
Six colon cancer cell lines, 13 colon tumors and ten normal colon tissues were analyzed for RNA expression using probes for c-myc, c-k-ras, c-myb, and c-fos and for the p53, TGF-alpha, and EGF receptor genes. No aberrant transcripts were detected. Levels of expression in tumors ranged from two-fold below that of normal tissue when the v-fos probe was used to 10 fold above the normal level when the c-myc probe was used. Enhanced c-myc expression was also observed in the cell lines. Southern and DNA dot blot analyses revealed c-myc amplification in three of the six cell lines.
Untawale
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S
Department of Genetics, University of Texas, M.D. Anderson Hospital and Tumor Institute, Houston 77030.
Blick
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M
D000199:Actins / Q000235:genetics; D000230:Adenocarcinoma / Q000235:genetics*; D002460:Cell Line; D003110:Colonic Neoplasms / Q000235:genetics*; D005786:Gene Expression Regulation; D006801:Humans; D007413:Intestinal Mucosa / Q000502:physiology; D009363:Neoplasm Proteins / Q000235:genetics; D009857:Oncogenes; D010455:Peptides / Q000235:genetics; D010750:Phosphoproteins / Q000235:genetics; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D012334:RNA, Neoplasm / Q000235:genetics; D015291:Transforming Growth Factors; D016159:Tumor Suppressor Protein p53
D000199:Actins; D009363:Neoplasm Proteins; D010455:Peptides; D010750:Phosphoproteins; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D016159:Tumor Suppressor Protein p53; D015291:Transforming Growth Factors