Characterization of surface markers of continuously growing murine resident macrophages.
Conditions have been described which allow an in vitro indefinite multiplication of differentiated murine macrophages (Lombard et al: Biol Cell 53, 219, 1985). R. and MAY-1 cell lines, which were obtained, respectively, from mouse (Balb/c) spleen and resident peritoneal macrophages, have been further characterized. They present at their surface, besides the Mac-1 antigen and Fc-receptor, a mannose receptor which was characterized for its binding properties. This receptor is responsive for a specific phagocytosis of mannosylated particles, i.e., mannosylated latex beads or oil droplets containing mannosylated bovine serum albumin. Moreover, R and MAY-1 cells present an ectoenzyme profile (NAD+ glycohydrolase and nucleotide pyrophosphatase) similar to those of the corresponding resident macrophages.
Muller
C D
CD
Université Louis Pasteur, Laboratoire de Chimie Enzymatique (UA CNRS 04 1182), Institut de Botanique, Strasbourg, France.
Lombard
Y
Y
Bartholeyns
J
J
Poindron
P
P
Schuber
F
F
D000818:Animals; D000954:Antigens, Surface / Q000032:analysis*; D002478:Cells, Cultured; D037181:Lectins, C-Type; D016169:Lymphocyte Function-Associated Antigen-1; D008264:Macrophages / Q000166:cytology*; D008358:Mannose; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins; D051379:Mice; D008807:Mice, Inbred BALB C; D010587:Phagocytosis; D011956:Receptors, Cell Surface; D011971:Receptors, Immunologic / Q000032:analysis
D000954:Antigens, Surface; D037181:Lectins, C-Type; D016169:Lymphocyte Function-Associated Antigen-1; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins; D011956:Receptors, Cell Surface; D011971:Receptors, Immunologic; D008358:Mannose