title: Infectious positive- and negative-strand transcript RNAs from bacteriophage Q beta cDNA clones.
abstract: Plasmids containing full-length cDNA copies of the Q beta RNA phage genome and flanking T7 promoters were constructed. Positive-strand Q beta RNA, generated by in vitro transcription of these plasmids with T7 RNA polymerase, was infectious to Escherichia coli spheroplasts. The Q beta replicase gene from the cloned DNA was subcloned and expressed in E. coli cells by means of a thermoinducible plasmid. Full-length, negative-strand Q beta transcripts were infectious when transfected into spheroplasts containing the induced replicase gene.
authors.affiliation: Biochemistry Department, University of Wisconsin, Madison 53706.
authors.forename: P N
authors.initials: PN
authors.lastname: Shaklee
authors.forename: J J
authors.initials: JJ
authors.lastname: Miglietta
authors.forename: A C
authors.initials: AC
authors.lastname: Palmenberg
authors.forename: P
authors.initials: P
authors.lastname: Kaesberg
mesh_terms: D003001:Cloning, Molecular; D003090:Coliphages / Q000235:genetics*; D004247:DNA / Q000235:genetics; D004926:Escherichia coli; D005814:Genes, Viral; D012367:RNA, Viral / Q000235:genetics*; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics; D013104:Spheroplasts; D014158:Transcription, Genetic; D014162:Transfection
chemical_list: D012367:RNA, Viral; D004247:DNA; D012324:RNA-Dependent RNA Polymerase