Metabolism of sulfur-containing amino acids by pregnant merino ewes.
The availability and utilization of cystine and methionine were measured in single-bearing Merino ewes on three occasions, approximately 90, 110 and 130 days after mating, and the effects on these traits of sulfur amino acids (SAA) infused into the abomasum were also measured. Two levels of SAA were infused containing 0.5 or 1.0 g day-1 organic sulfur with DL-methionine contributing two-thirds and L-cystine one-third of the supplementary sulfur. The quantity of the diet offered was increased at each occasion so as to maintain maternal liveweight. The rates of irreversible loss of both cystine and methionine from plasma increased as pregnancy advanced, but the ratios between the rates of irreversible loss and intake of digestible organic matter (DOMI) did not vary with stage of pregnancy. The average daily rates of irreversible loss of cystine and methionine by the ewes consuming the diet alone were 13.6 and 119 mmol kg-1 DOMI respectively. The average rates of irreversible loss of methionine (Im, mmol h-1) and of cystine (Ic, mmol h-1) were both linearly (P less than 0.05) related to the rate of infusion of organic sulfur into the abomasum (s, g day-1): Im = 2.44 (+/- 0.33) s + 1.28 (+/- 0.13); and Ic = 0.16 (+/- 0.02) s + 0.30 (+/- 0.01). Five per cent of the rate of irreversible loss of cystine arose from trans-sulfuration of methionine by ewes consuming the ration only, but greater percentages (14 and 22%) were observed when the ration was supplemented with SAA (P less than 0.05). These transfer quotients were not influenced by stage of pregnancy. The stage of pregnancy did not influence the concentration of cystine or methionine in the plasma, but the abomasal infusions of SAA significantly increased the concentration of both SAA. The ewes consuming the basal diet were in positive balance for both nitrogen and sulfur. The retention of nitrogen did not vary with stage of pregnancy (average (s.e.), 5.8 (0.9) g day-1), but that of sulfur increased from 0.6 to 1.0 and 1.3 g day-1 in periods 1, 2 and 3, respectively (P less than 0.05). The retentions of nitrogen (N, g day-1) and of sulfur (S, g day-1) were linearly and significantly related to the rate of infusion of organic sulfur into the abomasum (s, g day-1): N = 2.7 (+/- 0.7)s + 4.4 (+/- 0.3); and S = 0.49 (+/- 0.03)s + 0.72 (+/- 0.01).(ABSTRACT TRUNCATED AT 400 WORDS)
Williams
A J
AJ
Agricultural Research and Veterinary Centre, New South Wales Department of Agriculture, Orange.
Murison
R
R
Padgett
J
J
D000018:Abomasum / Q000378:metabolism; D000596:Amino Acids / Q000097:blood; D000818:Animals; D003553:Cystine / Q000008:administration & dosage / Q000097:blood / Q000378:metabolism*; D004032:Diet; D004063:Digestion; D005260:Female; D007700:Kinetics; D008715:Methionine / Q000008:administration & dosage / Q000097:blood / Q000378:metabolism*; D009584:Nitrogen / Q000378:metabolism; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000378:metabolism*; D012756:Sheep / Q000097:blood / Q000378:metabolism*; D013455:Sulfur / Q000008:administration & dosage / Q000378:metabolism; D013462:Sulfur Radioisotopes; D014316:Tritium; D014508:Urea / Q000097:blood; D014935:Wool / Q000254:growth & development / Q000378:metabolism
D000596:Amino Acids; D013462:Sulfur Radioisotopes; D014316:Tritium; D003553:Cystine; D013455:Sulfur; D014508:Urea; D008715:Methionine; D009584:Nitrogen