Glycosidase and phosphatase activities in U-937 and some clones and sublines.
1. The activities of nine glycosidases, lysozyme and acid and alkaline phosphatases were compared in the histiocytic lymphoma cell line U-937 and a set of clones and sublines derived from this line. 2. The patterns of the different enzyme activities and selected enzyme ratios have been used as a method to distinguish between different clones and sublines. 3. Sublines with high lysozyme levels were rich in most cell-bound glycosidases. 4. During long-term growth distinct enzyme patterns of individual lines were preserved. 5. The enzyme pattern during a cell culture growth cycle was basically stable.
Bjare
U
U
National Bacteriological Laboratory, Stockholm, Sweden.
Lundblad
G
G
Ivhed
I
I
Nilsson
K
K
D000135:Acid Phosphatase / Q000378:metabolism*; D000469:Alkaline Phosphatase / Q000378:metabolism*; D002455:Cell Division; D002999:Clone Cells / Q000201:enzymology; D006026:Glycoside Hydrolases / Q000378:metabolism*; D006801:Humans; D016403:Lymphoma, Large B-Cell, Diffuse / Q000201:enzymology* / Q000473:pathology; D009113:Muramidase / Q000378:metabolism; D013997:Time Factors; D014407:Tumor Cells, Cultured
D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D006026:Glycoside Hydrolases; D009113:Muramidase