Cellular ras activity and phospholipid metabolism.
Cellular ras activity has been neutralized in 3T3 cells by microinjection of a specific anti-ras monoclonal antibody. The injected antibody efficiently inhibited proliferation in cells treated with a phorbol ester and a calcium ionophore, or with prostaglandin F2 alpha. These treatments were designed to imitate the action of phospholipase C or of phospholipase A2. In addition, the highly efficient mitogenic potential of phosphatidic acid was inhibited by the injected antibody even more efficiently than was serum-induced proliferation. The close reliance of phospholipid-induced mitogenesis upon ras activity suggests that ras proteins are unlikely to function to control the action of a phospholipase.
Yu
C L
CL
Roche Institute of Molecular Biology, Roche Research Center, Nutley, New Jersey 07110.
Tsai
M H
MH
Stacey
D W
DW
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D015237:Dinoprost; D004987:Ethers / Q000494:pharmacology; D011905:Genes, ras; D015759:Ionomycin; D007476:Ionophores / Q000494:pharmacology; D008845:Microinjections; D008934:Mitogens / Q000494:pharmacology; D010712:Phosphatidic Acids / Q000494:pharmacology; D010741:Phospholipases A / Q000378:metabolism; D054467:Phospholipases A2; D010743:Phospholipids / Q000378:metabolism*; D011460:Prostaglandins F / Q000494:pharmacology; D011493:Protein Kinase C / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000276:immunology / Q000378:metabolism*; D016283:Proto-Oncogene Proteins p21(ras); D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D010738:Type C Phospholipases / Q000378:metabolism
D000911:Antibodies, Monoclonal; D004987:Ethers; D007476:Ionophores; D008934:Mitogens; D010712:Phosphatidic Acids; D010743:Phospholipids; D011460:Prostaglandins F; D011518:Proto-Oncogene Proteins; D015759:Ionomycin; D015237:Dinoprost; D011493:Protein Kinase C; D010741:Phospholipases A; D054467:Phospholipases A2; D010738:Type C Phospholipases; D016283:Proto-Oncogene Proteins p21(ras); D013755:Tetradecanoylphorbol Acetate