Development of amphotericin B liposomes bearing antibody specific to Candida albicans.
Liposomes expressing external antibody specific for Candida albicans and encapsulating amphotericin B were developed and characterized in this study. Antibody was first modified by the covalent attachment of palmitic acid residues. Liposomes were produced by reverse-phase evaporation and modified antibody was incorporated into these liposomes via the hydrophobic interaction between the palmitic acid and the phospholipids composing the liposomes. The liposomes were characterized as to the amount of amphotericin B by spectroscopy and for the presence of antibody by protein analysis and secondary immunolabeling by fluorescent and electron microscopic methods. Immunogold labeling showed that the antibody was being expressed externally on the liposomes in the electron microscopic studies and the specificity of these liposomes for C. albicans was observed by secondary immunofluorescence.
Hospenthal
D R
DR
Department of Botany, Michigan State University, East Lansing 48824.
Rogers
A L
AL
Mills
G L
GL
D000666:Amphotericin B / Q000008:administration & dosage* / Q000494:pharmacology; D000908:Antibodies, Fungal / Q000008:administration & dosage*; D000918:Antibody Specificity; D002176:Candida albicans / Q000187:drug effects / Q000276:immunology*; D002478:Cells, Cultured; D004337:Drug Carriers; D004912:Erythrocytes / Q000187:drug effects; D005455:Fluorescent Antibody Technique; D006801:Humans; D007150:Immunohistochemistry; D008081:Liposomes / Q000008:administration & dosage*; D008854:Microscopy, Electron
D000908:Antibodies, Fungal; D004337:Drug Carriers; D008081:Liposomes; D000666:Amphotericin B