title: Quantitative correlation of parasitological and serological techniques for the diagnosis of Trypanosoma congolense infection in cattle.
abstract: A comparison was made between serological and parasitological techniques for the diagnosis of bovine trypanosomiasis in Zambia. Overall sero-prevalence rates as determined by IFAT and ELISA were respectively 2.7-fold and 2.9-fold greater then the percentage of samples found positive with the dark ground/phase contrast buffy coat technique (DG). The results obtained by the two serological techniques were found to be closely correlated (94.2%) agreement) and titres obtained by ELISA tended to be slightly higher than those obtained by IFAT. Linear regression analysis of the results obtained by the IFAT and DG techniques revealed a highly significant correlation. This finding would permit the use of only one of the techniques in an epidemiological survey and to extrapolate the results from the regression line.
authors.affiliation: UNDP/FAO, Animal Disease Control Project, Lusaka, Zambia.
authors.forename: F
authors.initials: F
authors.lastname: Jongejan
authors.forename: C J
authors.initials: CJ
authors.lastname: Ooijen
authors.forename: D
authors.initials: D
authors.lastname: Zivkovic
authors.forename: A A
authors.initials: AA
authors.lastname: ter Huurne
mesh_terms: D000818:Animals; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D012698:Serologic Tests / Q000662:veterinary; D014348:Trypanosoma congolense; D014353:Trypanosomiasis, African / Q000175:diagnosis / Q000662:veterinary; D014354:Trypanosomiasis, Bovine / Q000175:diagnosis*; D015024:Zambia