An enzyme immunoassay for the flow cytometer (FEIA).
This report describes the use of a fluorescent activated cell sorter (FACS) in combination with an enzyme immunosorbent assay (EIA). This combination of techniques expands the versatility of the flow cytometer. It introduces a new set of fluorescent enzyme products for antigen detection. These highly substantive fluorescent compounds permit the flow cytometer to quantify cell-EIA reactions and also to delineate subpopulations of cells with different quantities of surface antigens. Because the FEIA product is colored, as well as fluorescent, a simple light microscope may also be used to define the distribution of the label on the cell surface. These techniques have been applied to the examination of antigens on human erythrocytes, human T cell lymphoma cells (H9), and to surface markers on the tissue culture cell line K562.
Makler
M T
MT
Laboratory and Research Service, Veterans Administration Medical Center, Portland, OR 97201.
Bakke
A C
AC
Piper
R C
RC
D000941:Antigens / Q000032:analysis; D002460:Cell Line; D004912:Erythrocytes / Q000032:analysis; D005434:Flow Cytometry / Q000379:methods* / Q000592:standards; D006801:Humans; D007124:Immunoenzyme Techniques / Q000592:standards; D004915:Leukemia, Erythroblastic, Acute / Q000032:analysis / Q000473:pathology; D008223:Lymphoma / Q000032:analysis / Q000473:pathology; D013601:T-Lymphocytes / Q000032:analysis
D000941:Antigens