Restriction endonucleases in Clostridium pasteurianum ATCC 6013 and C. thermohydrosulfuricum DSM 568.
A small collection of clostridia was surveyed for type II restriction endonucleases. Enzymes were detected in two organisms. Clostridium pasteurianum ATCC 6013 contains an isoschizomer of ThaI (FnuDII) [5'-CGCG-3'] and preliminary evidence suggests that cleavage generates blunt-ended fragments. Clostridium thermohydrosulfuricum DSM 568 contains an isoschizomer of MboI (Sau3A) [5'-GATC-3'] that is inactive on dam methylated substrates. The DNA of this latter organism shows dam methylation.
Richards
D F
DF
Department of Botany and Microbiology, University College of Wales, Dyfed, UK.
Linnett
P E
PE
Oultram
J D
JD
Young
M
M
D003013:Clostridium / Q000201:enzymology* / Q000235:genetics; D015252:Deoxyribonucleases, Type II Site-Specific / Q000032:analysis*; D004587:Electrophoresis, Agar Gel
D015252:Deoxyribonucleases, Type II Site-Specific