[Sex determination in hair roots without sheath: proposal of a method for preparation of specimens].
Japanese authors have described a procedure for the sex determination of human hair roots without epithelial sheath, paraffin embedded and fluorescent stained for cortical cell nuclei Y or X sex chromatins. This method however is not sufficiently practical for forensic science purposes: it is difficult and not guaranteed to obtain suitable longitudinal hair sections with possible loss of evidence particularly serious when the questioned hair is only one. The authors, researching Y-bodies with Quinacrine, present an alternative technique of preparation of specimens without embedding which is simple and quick, reproducible and satisfying in results and that avoids loss of material.
Pappalardo
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Istituto di medicina legale e delle assicurazioni Università, Bologna.
Pelotti
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S
D005456:Fluorescent Dyes; D006197:Hair / Q000032:analysis*; D006801:Humans; D012732:Sex Determination Analysis; D013048:Specimen Handling / Q000379:methods*; D014998:Y Chromosome / Q000032:analysis
D005456:Fluorescent Dyes
La diagnosi di sesso sulle radici pilifere prive di guaina: proposta di un metodo per allestire i preparati.