title: Human cathepsin B and cysteine proteinase inhibitors (CPIs) in inflammatory and metabolic joint diseases.
abstract: Synovial fluid of patients with different inflammatory and metabolic joint diseases contains low-molecular CPIs (stefins and cystatins) and high-molecular CPIs (kininogens). An additional inhibitory fragment with a molecular mass of about 20 kDa, which is a part of the kininogen molecule, has been detected. Cathepsin B and cystatin C were determined by ELISA test in 47 patients with rheumatoid arthritis, seronegative spondylarthritis, osteoarthritis, undifferentiated arthritis and gout. A significantly higher amount of cathepsin B was found in patients with rheumatoid arthritis. The elevation of cathepsin B was accompanied by an increased amount of cystatin C.
authors.affiliation: Department of Biochemistry, J. Stefan Institute, Ljubljana.
authors.forename: B
authors.initials: B
authors.lastname: Lenarcic
authors.forename: D
authors.initials: D
authors.lastname: Gabrijelcic
authors.forename: B
authors.initials: B
authors.lastname: Rozman
authors.forename: M
authors.initials: M
authors.lastname: Drobnic-Kosorok
authors.forename: V
authors.initials: V
authors.lastname: Turk
mesh_terms: D000595:Amino Acid Sequence; D002401:Cathepsin B / Q000502:physiology*; D002846:Chromatography, Affinity; D015853:Cysteine Proteinase Inhibitors; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007249:Inflammation / Q000503:physiopathology; D007592:Joint Diseases / Q000503:physiopathology*; D008659:Metabolic Diseases / Q000503:physiopathology; D008969:Molecular Sequence Data; D011480:Protease Inhibitors / Q000032:analysis / Q000302:isolation & purification / Q000502:physiology*; D013582:Synovial Fluid / Q000032:analysis
chemical_list: D015853:Cysteine Proteinase Inhibitors; D011480:Protease Inhibitors; D002401:Cathepsin B