title: Thymine dimer repair in fibroblasts of patients with dysplastic naevus syndrome (DNS).
abstract: Dysplastic naevus syndrome (DNS) is frequently observed in association with familial melanoma and xeroderma pigmentosum (XP), but the role of UV-light in the development of DNS has not been elucidated. Previous work has shown that UV-induced unscheduled DNA synthesis is associated with the early loss of antigenicity observed in immunoassays using a monoclonal antibody specific for thymine-thymine dimers. We now show that the rate of loss of antigenicity, which reflects the relative amount of bound antibody, observed during the first 60 min following 10 Jm-2 UVC irradiation is significantly reduced (p = 0.02) in cultures of fibroblasts from 7 out of 8 DNS patients compared with the results from cells of a group of 30 healthy volunteers. This observation suggests an early event in excision repair is altered in the majority of DNS patients.
authors.affiliation: Dept of Research, University Clinics, Kantonsspital, Basel, Switzerland.
authors.forename: M
authors.initials: M
authors.lastname: Roth
authors.forename: J M
authors.initials: JM
authors.lastname: Boyle
authors.forename: H
authors.initials: H
authors.lastname: Müller
mesh_terms: D000328:Adult; D000951:Antigens, Neoplasm / Q000276:immunology; D004260:DNA Repair; D004416:Dysplastic Nevus Syndrome / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D005260:Female; D005347:Fibroblasts / Q000276:immunology / Q000473:pathology / Q000502:physiology; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D011740:Pyrimidine Dimers / Q000235:genetics*; D012867:Skin / Q000276:immunology / Q000473:pathology / Q000503:physiopathology*
chemical_list: D000951:Antigens, Neoplasm; D011740:Pyrimidine Dimers