Enhancement of growth of primary metastatic fresh human tumors of the nervous system by epidermal growth factor in serum-free short term culture.
Using the adhesive tumor cell culture system, we studied the effect of epidermal growth factor (EGF) on 16 primary and 7 metastatic fresh human tumors of the nervous system cultured in serum-free and serum-supplemented media at low cell density. In serum-free conditions, EGF significantly enhanced the growth of glial tumor cells. This positive effect was less pronounced for metastases to the brain. No significant enhancement was observed for the other tumor types (primitive neuroectodermal tumors and various tumors of neuroepithelial/mesenchymal origin). The addition of serum obscured this effect of EGF, even at the lowest cell densities. In 7 tumors, the simultaneous addition of platelet-derived growth factor did not enhance the EGF response. Subtypes of brain tumors respond to EGF in vitro under serum-free conditions.
Frappaz
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Department of General Surgery, University of Texas M. D. Anderson Cancer Center, Houston.
Singletary
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Spitzer
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Yung
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D001932:Brain Neoplasms / Q000378:metabolism*; D002455:Cell Division / Q000187:drug effects; D002648:Child; D002675:Child, Preschool; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D005910:Glioma / Q000378:metabolism*; D006801:Humans; D008577:Meningeal Neoplasms / Q000378:metabolism*; D008579:Meningioma / Q000378:metabolism*; D014407:Tumor Cells, Cultured / Q000166:cytology* / Q000187:drug effects
D004815:Epidermal Growth Factor