Antigen presentation by liposomes as model system for T-B cell interaction.
Using the earlier established liposome system for antigen presentation, in which liposomes bearing major histocompatibility complex (MHC)-class II molecules inserted into and protein antigen covalently linked to the membrane were found to be sufficient for an antigen-specific and MHC-restricted activation of T cells, the minimal requirements for T-B cell interaction were investigated. Liposomes carrying MHC class II molecules and antigen-specific monoclonal antibodies were constructed and tested for their ability to present soluble antigen to T cells. With the antigens lactate dehydrogenase B and pigeon cytochrome c, a specific stimulation of T cell clones and hybridomas could be obtained. These results demonstrate the possibility of a direct involvement of the immunoglobulins on the B cell surface in their interaction with T cells.
Walden
P
P
Abteilung Immungenetik, Max-Planck-Institut für Biologie, Tübingen, FRG.
D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology; D000941:Antigens / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology*; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D008081:Liposomes; D008213:Lymphocyte Activation; D008211:Lymphocyte Cooperation; D008285:Major Histocompatibility Complex; D051379:Mice; D008815:Mice, Inbred Strains; D008954:Models, Biological; D011947:Receptors, Antigen, B-Cell / Q000276:immunology / Q000502:physiology; D011948:Receptors, Antigen, T-Cell / Q000502:physiology
D000941:Antigens; D000949:Histocompatibility Antigens Class II; D008081:Liposomes; D011947:Receptors, Antigen, B-Cell; D011948:Receptors, Antigen, T-Cell