Development of immunoreagents to ciliary zonules that react with protein components of elastic fiber microfibrils and with elastin-producing cells.
We describe the generation of a monoclonal antibody library to ocular zonule components and the characterization of three monoclonal antibodies: 1) one specific for microfibrillar associated glycoprotein (MAGP), a component of both ocular zonules and microfibrils of elastin fibers, 2) an antibody to an as yet unidentified 70,000 dalton antigen that is present in abundance in the extracellular matrix (ECM) of elastin-producing cells, and 3) an antibody reacting with the 67000 dalton subunit of the elastin receptor. The presence of antigenic determinants common to the ocular zonule and elastic fiber microfibrils suggests that zonules, which can be obtained in relatively pure form, can provide a valuable resource for characterizing proteins common to both microfibrillar structures.
Mecham
R P
RP
Department of Medicine, Jewish Hospital, Washington University Medical Center, St. Louis, MO 63110.
Hinek
A
A
Cleary
E G
EG
Kucich
U
U
Lee
S J
SJ
Rosenbloom
J
J
D000818:Animals; D000911:Antibodies, Monoclonal; D002356:Cartilage / Q000648:ultrastructure; D002417:Cattle; D003285:Contractile Proteins / Q000032:analysis* / Q000276:immunology; D004547:Elastic Tissue / Q000378:metabolism; D004549:Elastin / Q000032:analysis / Q000096:biosynthesis* / Q000276:immunology; D005109:Extracellular Matrix / Q000648:ultrastructure; D016326:Extracellular Matrix Proteins; D005123:Eye / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D008854:Microscopy, Electron; D008970:Molecular Weight; D000072260:RNA Splicing Factors
D000911:Antibodies, Monoclonal; D003285:Contractile Proteins; D016326:Extracellular Matrix Proteins; D000072260:RNA Splicing Factors; C016598:microfibrillar protein; D004549:Elastin