Inhibition of c-H-ras oncogene induced transformation of rat embryo fibroblasts by cotransfected polynucleotides containing alternating purine-pyrimidine sequences.
When Rat 6 cultures were cotransfected with an activated c-H-ras oncogene (pT24) and poly(dG-m5dC), a synthetic polymer that has the potential to form Z DNA, there was marked inhibition of cell transformation. Cotransfection of pT24 DNA with poly(dG-dC) caused somewhat less inhibition, poly(dA-dC). (dG-dT) caused moderate inhibition, and poly(dG). (dC) exerted negligible inhibition. Evidence was obtained that the inhibition seen with poly(dG-m5dC) was not simply due to an inhibition of cellular uptake of the pT24 DNA. Our results suggest that certain polymers that have the potential to form Z DNA can inhibit the integration and expression of a transfected oncogene.
Banerjee
R
R
Institute of Cancer Research and Comprehensive Cancer Center, College of Physicians and Surgeons, Columbia University, New York, NY 10032.
Hsiao
W L
WL
Weinstein
I B
IB
Grunberger
D
D
D000818:Animals; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D011905:Genes, ras / Q000187:drug effects; D010957:Plasmids; D011089:Polydeoxyribonucleotides / Q000494:pharmacology*; D011687:Purines; D011743:Pyrimidines; D013329:Structure-Activity Relationship; D014162:Transfection
D011089:Polydeoxyribonucleotides; D011687:Purines; D011743:Pyrimidines