Epidermal growth factor (EGF) receptor gene transcription. Requirement for Sp1 and an EGF receptor-specific factor.
We have studied in vitro transcription of the human epidermal growth factor (EGF) receptor proto-oncogene using nuclear extracts of A431 human epidermoid carcinoma cells, which overproduce the EGF receptor. With the in vitro system we found that Sp1 and other trans-acting factors bound to the EGF receptor promoter regions and are required for maximal expression. Fractionation showed that a DEAE-Sepharose fraction (BA) contained a novel factor, which specifically stimulated EGF receptor transcription 5- to 10-fold. The molecular mass of the native form of the factor is about 270-kDa based on its migration on Sephacryl S-300. This factor may activate transcription of the proto-oncogene through a weak or indirect interaction with the DNA template.
Kageyama
R
R
Laboratory of Molecular Biology, National Cancer Institute, Bethesda, Maryland 20892.
Merlino
G T
GT
Pastan
I
I
D001483:Base Sequence; D002294:Carcinoma, Squamous Cell / Q000235:genetics; D002460:Cell Line; D002872:Chromosome Deletion; D004268:DNA-Binding Proteins / Q000378:metabolism*; D003850:Deoxyribonuclease I / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D066246:ErbB Receptors / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009154:Mutation; D011401:Promoter Regions, Genetic; D000090063:Proto-Oncogene Mas; D011519:Proto-Oncogenes; D016329:Sp1 Transcription Factor; D014157:Transcription Factors / Q000378:metabolism*; D014158:Transcription, Genetic
D004268:DNA-Binding Proteins; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D016329:Sp1 Transcription Factor; D014157:Transcription Factors; D066246:ErbB Receptors; D003850:Deoxyribonuclease I