Expression of two kinds of cytochrome P-450(11 beta) mRNA in bovine adrenal cortex.
Using pcP-450(11 beta)-2 cDNA (Morohashi et al. (1987) J. Biochem. 102, 559-568) as the probe, a different cDNA clone, pcP-450(11 beta)-3, was isolated from a cDNA library of bovine adrenal cortex. The restriction enzyme map of pcP-450(11 beta)-3 was highly homologous but not identical with that of pcP-450(11 beta)-2. Nucleotide sequence determination revealed the substitutions of 14 nucleotides and 3 amino acids between pcP-450(11 beta)-2 and -3. Blotting analysis involving two different oligonucleotide probes specific to these two cDNAs indicated that at least two kinds of P-450(11 beta) mRNA were expressed in individual animals and that at least two kinds of P-450(11 beta) genes exist in the bovine genome.
Kirita
S
S
Department of Biology, Faculty of Science, Kyushu University, Fukuoka.
Morohashi
K
K
Hashimoto
T
T
Yoshioka
H
H
Fujii-Kuriyama
Y
Y
Omura
T
T
D000302:Adrenal Cortex / Q000201:enzymology*; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D002417:Cattle; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D013252:Steroid 11-beta-Hydroxylase / Q000235:genetics*; D013250:Steroid Hydroxylases / Q000235:genetics*
D015345:Oligonucleotide Probes; D004247:DNA; D013250:Steroid Hydroxylases; D013252:Steroid 11-beta-Hydroxylase