Antibodies to a short synthetic peptide cross-react with human recombinant interleukin 1 alpha.
An antiserum to human interleukin 1 alpha has been prepared by immunizing a sheep with a short synthetic peptide (Mr 1427) conjugated to keyhole limpet haemocyanin using the heterobifunctional cross-linking agent N-succinimidyl bromoacetate. The peptide was selected from a highly hydrophilic region corresponding to residues 169-179 of the cDNA-derived sequence. Two additional peptides corresponding to residues 194-207 and 224-233 failed to elicit cross-reacting antibodies.
Davies
M E
ME
Tissue Physiology Department, Strangeways Research Laboratory, Worts Causeway, Cambridge, U.K.
Knight
C G
CG
Mativi
B Y
BY
D000818:Animals; D000906:Antibodies / Q000302:isolation & purification; D003429:Cross Reactions; D006801:Humans; D015151:Immunoblotting; D007375:Interleukin-1 / Q000276:immunology*; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology*; D012756:Sheep
D000906:Antibodies; D007375:Interleukin-1; D010455:Peptides