The N terminus of laminin A chain is homologous to the B chains.
A major proteolytic fragment (E1/E1-4) of the basement membrane protein laminin, comprising the three short arms with some terminal globules missing, was isolated by elastase digestion, and partial protein sequence data were determined for several tryptic peptides. Sequences which corresponded to A-chain structures were used to synthesize oligonucleotides for the construction and screening of a primer-extended cDNA library from mouse PYS-2 cells. A clone of 1.1 kb was obtained and shown by sequencing to correspond to the 5' end of the 10-kb mRNA of the A chain of laminin. The clone contains 77 nucleotides of 5' untranslated sequence and a region coding for 334 amino acids, including a presumptive signal peptide of 24 amino acids. The sequence is 30% homologous to the corresponding N-terminal part of the B1 chain of laminin, suggesting the same structure for both domains. The data present further evidence for a recent structural model which postulates that each of the three laminin polypeptide chains forms a distinct short arm.
Hartl
L
L
Max-Planck-Institut für Biochemie, Martinsried, Federal Republic of Germany.
Oberbäumer
I
I
Deutzmann
R
R
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001485:Basement Membrane / Q000032:analysis; D005075:Biological Evolution; D004247:DNA / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D005815:Genetic Code; D007797:Laminin / Q000032:analysis / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D009374:Neoplasms, Experimental / Q000032:analysis; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000032:analysis; D012689:Sequence Homology, Nucleic Acid
D007797:Laminin; D012333:RNA, Messenger; D004247:DNA