Metabolic fate of N1-methyladenine in yeast auxotrophic to adenine.
The metabolic fate of N1-methyladenine in yeast with respect to its incorporation into RNA has been studied. Chromatographic analysis of the PCA-soluble and -insoluble fractions of cells grown in the combined presence of adenine and 3H-labeled N1-methyladenine show that (a) N1-methyladenine can enter the cells, (b) however, it is very poorly utilized by the salvage pathway for nucleic acid synthesis and (c) the inhibition occurs probably at the first stage of conversion of the methylated base to the corresponding nucleotide.
Murthy
M S
MS
Division of Radiological Protection, Bhabha Atomic Research Centre, Trombay, Bombay, India.
Deorukhakar
V V
VV
D000225:Adenine / Q000031:analogs & derivatives* / Q000378:metabolism*; D000227:Adenine Nucleotides / Q000138:chemical synthesis; D012331:RNA, Fungal / Q000096:biosynthesis*; D012441:Saccharomyces cerevisiae / Q000378:metabolism*
D000227:Adenine Nucleotides; D012331:RNA, Fungal; C008407:1-methyladenine; D000225:Adenine