Proteus mirabilis urease. Partial purification and inhibition by boric acid and boronic acids.
Urease was purified 800-fold and partially characterized from Proteus mirabilis, the predominant microorganism associated with urinary stones. Boric acid is a rapid reversible competitive inhibitor of urease. The pH-dependence of inhibition exhibited pKa values of 6.25 and 9.3, where the latter value is probably due to the inherent pKa of boric acid. Three boronic acids also were shown to inhibit urease competitively.
Breitenbach
J M
JM
Department of Microbiology and Public Health, Michigan State University, East Lansing 48824-1101.
Hausinger
R P
RP
D001888:Boric Acids / Q000494:pharmacology; D001897:Boronic Acids / Q000494:pharmacology; D002853:Chromatography, Liquid; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D011513:Proteus mirabilis / Q000201:enzymology*; D014510:Urease / Q000037:antagonists & inhibitors / Q000302:isolation & purification*
D001888:Boric Acids; D001897:Boronic Acids; D014510:Urease; C032688:boric acid