title: Cloning and expression of secreted antigens of Clostridium difficile in Escherichia coli.
abstract: The feasibility of the cloning and expression of Clostridium difficile antigens in Escherichia coli was investigated. The expression of a limited number of cloned clostridial antigens under the control of clostridial promoter elements in E. coli was observed.
authors.affiliation: Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis 46223.
authors.forename: D C
authors.initials: DC
authors.lastname: Dailey
authors.forename: R H
authors.initials: RH
authors.lastname: Schloemer
mesh_terms: D000942:Antigens, Bacterial / Q000235:genetics* / Q000302:isolation & purification; D003001:Cloning, Molecular; D003013:Clostridium / Q000235:genetics / Q000276:immunology*; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D014162:Transfection
chemical_list: D000942:Antigens, Bacterial