The use of a selective staphylococcal broth v direct plating for the recovery of Staphylococcus aureus.
Nine hundred seventy-two cultures taken from the external nares and the vaginal vestibules of 54 women for the isolation of Staphylococcus aureus were studied. The swabs were plated directly to a trypticase soy agar plate containing 5% sheep blood and were then placed into a selective staphylococcal broth. Both culture methods were compared for the ability to recover S aureus. Twenty percent (26/131) and 66% (38/58) of the S aureus-positive cultures taken from the nares and vagina respectively were cultured from the selective broth only. We believe that a selective staphylococcal broth should be used in addition to routine culture techniques to isolate S aureus from infection control surveillance cultures.
Sautter
R L
RL
Department of Biological Sciences, Wayne State University, Detroit, Michigan.
Brown
W J
WJ
Mattman
L H
LH
D000293:Adolescent; D000328:Adult; D001431:Bacteriological Techniques; D003470:Culture Media; D005260:Female; D006801:Humans; D008875:Middle Aged; D009666:Nose / Q000382:microbiology; D013211:Staphylococcus aureus / Q000302:isolation & purification*; D014621:Vagina / Q000382:microbiology
D003470:Culture Media