Immunocytochemical identification of bovine Langerhans cells by use of a monoclonal antibody directed against class II MHC antigens.
We undertook a study to develop a reliable light microscopic technique for identifying Langerhans cells (LC) in bovine epidermis. Monoclonal antibodies (MCA) detecting bovine class II MHC antigens were used in conjunction with an avidin-biotin-peroxidase complex (ABC) immunocytochemical staining method. The specificity of the MCA for LC was confirmed ultrastructurally by use of gold-labeled second antibody. Epidermal sheets and epidermal single-cell suspensions examined by light microscopy confirm that bovine epidermal LC express class II antigens. Anti-bovine class II MCA is a dependable reagent for identification of LC in normal bovine epidermis.
Bryan
L A
LA
Department of Veterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Canada.
Griebel
P J
PJ
Haines
D M
DM
Davis
W C
WC
Allen
J R
JR
D000818:Animals; D000911:Antibodies, Monoclonal; D002417:Cattle; D000078404:Epidermal Cells; D006649:Histocompatibility Antigens / Q000032:analysis* / Q000276:immunology; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D007801:Langerhans Cells / Q000166:cytology* / Q000276:immunology; D008854:Microscopy, Electron
D000911:Antibodies, Monoclonal; D006649:Histocompatibility Antigens