[Monoclonal antibodies--new probes for diagnosis and therapy. Their use as an example of the micrometastasizing of solid tumors].
Monoclonal antibody CK2, recognizing component No. 18, appeared to be the most suitable reagent for the detection of epithelial tumor cells in bone marrow. Its specificity was confirmed in a double-marker staining procedure (combination of APAAP-technique and radioautography). CK2 positive cells were demonstrated not to reveal any cross-reactivity with an antibody directed against the "leucocyte common antigen". A significant correlation between the presence of epithelial tumor cells in bone marrow and certain conventional risk factors was found. A more detailed phenotypic characterisation could demonstrate the expression of proliferation associated antigens on these cells. Furthermore in an immunotherapeutic approach with monoclonal antibody 17-1A, labelling of the disseminated tumor cells in bone marrow after infusion of the antibody was shown.
Schlimok
G
G
Institut für Immunologie, Universität München.
Funke
I
I
Bock
B
B
Schweiberer
B
B
Riethmüller
G
G
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm / Q000032:analysis*; D001853:Bone Marrow / Q000473:pathology*; D002277:Carcinoma / Q000175:diagnosis*; D006801:Humans; D009362:Neoplasm Metastasis / Q000175:diagnosis*; D011379:Prognosis
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm
Monoklonale Antikörper--neue Sonden für Diagnose und Therapie. Ihr Einsatz am Beispiel der Mikrometastasierung solider Tumoren.