title: Rapid latex agglutination assay using recombinant envelope polypeptide for the detection of antibody to the HIV.
abstract: Unscreened blood transfusions continue to be one of the major modes of transmission of the human immunodeficiency virus (HIV) in developing countries, such as in Central Africa, where 5% to 18% of blood donors are HIV seropositive. We evaluated a rapid latex agglutination assay using a novel recombinant envelope polypeptide of HIV for the detection of HIV antibodies among 2820 blood donors and clinical patients from diverse geographic regions, including on-site testing in Central Africa of 1600 blood donors. Overall, 29.2% of the serum samples were positive by Western blot assay. On a single determination, the latex agglutination slide test was found to be highly sensitive and specific compared with Western blot results in these populations with a relatively high prevalence of infection. Use of this assay will allow the immediate implementation of serologic screening for HIV in developing areas of the world, where standard screening procedures are impractical or not available, and in many other clinical settings, such as sexually transmitted diseases clinics and hospitals, where testing and counseling could be promptly implemented.
authors.affiliation: Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, Bethesda, Md.
authors.forename: T C
authors.initials: TC
authors.lastname: Quinn
authors.forename: C H
authors.initials: CH
authors.lastname: Riggin
authors.forename: R L
authors.initials: RL
authors.lastname: Kline
authors.forename: H
authors.initials: H
authors.lastname: Francis
authors.forename: K
authors.initials: K
authors.lastname: Mulanga
authors.forename: M G
authors.initials: MG
authors.lastname: Sension
authors.forename: A S
authors.initials: AS
authors.lastname: Fauci
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000635:transmission; D001782:Blood Donors; D015023:Democratic Republic of the Congo; D003906:Developing Countries; D006679:HIV Seropositivity / Q000175:diagnosis* / Q000453:epidemiology; D006801:Humans; D007841:Latex Fixation Tests / Q000379:methods*; D011994:Recombinant Proteins / Q000276:immunology; D012680:Sensitivity and Specificity; D013997:Time Factors; D065227:Transfusion Reaction; D014481:United States; D014759:Viral Envelope Proteins / Q000276:immunology
chemical_list: D011994:Recombinant Proteins; D014759:Viral Envelope Proteins