title: murH, a new genetic locus in Escherichia coli involved in cell wall peptidoglycan biosynthesis.
abstract: A temperature-sensitive mutant of Escherichia coli defective in peptidoglycan synthesis was characterized. The incorporation of radiolabeled meso-diaminopimelate into peptidoglycan by the mutant was inhibited at the restrictive growth temperature, resulting in autolysis. The defective step appeared to be part of the terminal stage in peptidoglycan synthesis involving the incorporation of disaccharide peptide units into the wall peptidoglycan. The mutation was assigned to a new locus, designated murH, at 99.2 min on the E. coli linkage map.
authors.affiliation: Department of Biochemistry and Microbiology, University of Victoria, British Columbia, Canada.
authors.forename: D
authors.initials: D
authors.lastname: Dai
authors.forename: E E
authors.initials: EE
authors.lastname: Ishiguro
mesh_terms: D002473:Cell Wall; D002874:Chromosome Mapping; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism / Q000648:ultrastructure; D005798:Genes, Bacterial; D009154:Mutation; D010457:Peptidoglycan / Q000096:biosynthesis*; D010641:Phenotype; D013696:Temperature
chemical_list: D010457:Peptidoglycan