Fluorescence-activated cell-sorting analysis of fibronectin peptides binding to Trypanosoma cruzi trypomastigotes.
The binding of synthetic peptides modeled from the sequence of the cell attachment site of fibronectin to T. cruzi trypomastigote surface receptors was investigated by fluorescence-activated cell-sorting analysis using fluorescein-labeled peptides. Peptides with the sequence Arg-Gly-Asp-Ser bound to the parasite surface. A low percentage of fresh parasites recently liberated from infected fibroblasts had the capacity to bind the peptide. In contrast, these parasites showed a time-dependent several-fold increase in their ability to bind the Arg-Gly-Asp-Ser-containing peptides during extracellular incubation. From these observations, it appears that the expression of surface receptors on a particular, mature stage of the parasite parallels its ability to adhere to and infect host cells.
Quaissi
M A
MA
Centre d'Immunologie et de Biologie Parasitaire, Unité Mixte INSERM 167--CNRS 624, Lille, France.
Kusnierz
J P
JP
Gras-Masse
H
H
Drobecq
H
H
Velge
P
P
Cornette
J
J
Capron
A
A
Tartar
A
A
D000595:Amino Acid Sequence; D000818:Animals; D002469:Cell Separation; D005353:Fibronectins / Q000032:analysis* / Q000378:metabolism; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000032:analysis* / Q000378:metabolism; D014349:Trypanosoma cruzi / Q000032:analysis / Q000378:metabolism*
D005353:Fibronectins; D010446:Peptide Fragments