Mobilization of arachidonic acid from diacyl and ether-linked phospholipids in FMLP stimulated alveolar macrophages.
Exposure of [1 14C]AA labeled guinea-pig alveolar macrophages to FMLP for 15 min induced an extensive mobilization of AA from phospholipids. PC and PI mainly contributed to the AA release, and labeled PE remained unchanged. Analysis of ether-linked phospholipids showed a significant breakdown of labeled diacyl and alkyl-acyl PC and an increase in labeled alkenyl-acyl PE.
Masliah
J
J
Laboratoire de Biochimie, C.H.U.-Saint-Antoine, Paris, France.
Bachelet
M
M
Colard
O
O
Bereziat
G
G
Vargaftig
B B
BB
D000818:Animals; D001095:Arachidonic Acids / Q000378:metabolism*; D002250:Carbon Radioisotopes; D006168:Guinea Pigs; D007700:Kinetics; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology*; D010743:Phospholipids / Q000378:metabolism*; D011865:Radioisotope Dilution Technique
D001095:Arachidonic Acids; D002250:Carbon Radioisotopes; D010743:Phospholipids; D009240:N-Formylmethionine Leucyl-Phenylalanine