Anti-Ig antibody inhibits the phorbol ester-induced stimulation of peritoneal B cells.
Peritoneal B cells are stimulated to enter S phase by phorbol esters acting alone, in the absence of a co-mitogen. Anti-Ig antibody inhibited the stimulation of peritoneal B cells induced by phorbol esters and this inhibition was apparent throughout the time course of PMA-induced stimulation. Assessment of inhibition after depletion of Ly-1+ B cells suggested that PMA-induced stimulation of both Ly-1+ and Ly-1- B cells was affected by anti-Ig. Inhibition produced by anti-Ig was substantially reduced after even a brief delay in the addition of antibody to PMA-stimulated B cells. Anti-Ig antibody may counteract early events triggered by PMA within minutes of peritoneal B cell stimulation.
Rothstein
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Evans Memorial Department of Clinical Research, Boston University Medical Center, MA 02118.
Kolber
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D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000502:physiology*; D000950:Antigens, Ly; D001402:B-Lymphocytes / Q000145:classification / Q000187:drug effects / Q000276:immunology*; D001667:Binding, Competitive; D004306:Dose-Response Relationship, Immunologic; D007700:Kinetics; D008213:Lymphocyte Activation / Q000187:drug effects*; D051379:Mice; D008807:Mice, Inbred BALB C; D010529:Peritoneal Cavity; D010641:Phenotype; D011493:Protein Kinase C / Q000502:physiology; D013154:Spleen; D013755:Tetradecanoylphorbol Acetate / Q000037:antagonists & inhibitors*; D013997:Time Factors
D000888:Antibodies, Anti-Idiotypic; D000950:Antigens, Ly; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate