Antagonistic effects of laminin and fibronectin in cell-to-cell and cell-to-matrix interactions in MCF-7 cultures.
During morphogenesis, tumor progression and metastasis, cell adhesion, dissociation, and migration result from a complex balance between cell-to-cell and cell-to-matrix interactions. Two different organization patterns of MCF-7 cells were induced by different extracellular matrix proteins. When plated on plastic or polymeric type I collagen gel used as a model of interstitial matrix, MCF-7 cells spread and grew in monolayer. When cultured on a solid gel of basement membrane (BM) proteins (85% laminin) used as a model of BM, cells formed clusters attached to the matrix. Matrix proteins regulated these two types of cell organization by preferentially promoting cell-to-cell or cell-support interactions. On plastic in the presence of soluble laminin or on laminin-coated dishes, cells also formed clusters. Addition of soluble fibronectin induced spreading of the cells, suggesting that laminin and fibronectin have competitive antagonistic effects on MCF-7 cell morphology. Antilaminin antibodies inhibited cluster formation and attachment, emphasizing the important role of this glycoprotein not only in promoting cluster attachment but also in cell-to-cell contact formation. Such effects of extracellular matrix proteins could play significant roles in tumor progression and metastasis.
Noel
A
A
Laboratory of Experimental Dermatology and Pathophysiology of Pregnancy, University of Liège, Belgium.
Callé
A
A
Emonard
H
H
Nusgens
B
B
Foidart
J M
JM
Lapiere
C M
CM
D001943:Breast Neoplasms / Q000378:metabolism / Q000648:ultrastructure*; D002448:Cell Adhesion; D002450:Cell Communication; D002460:Cell Line; D005109:Extracellular Matrix / Q000378:metabolism*; D005260:Female; D005353:Fibronectins / Q000494:pharmacology*; D006801:Humans; D007797:Laminin / Q000494:pharmacology*; D008855:Microscopy, Electron, Scanning; D008858:Microscopy, Phase-Contrast; D009024:Morphogenesis; D009362:Neoplasm Metastasis
D005353:Fibronectins; D007797:Laminin