Thrombospondin is present in articular cartilage and is synthesized by articular chondrocytes.
Thrombospondin, a multifunctional adhesive glycoprotein originally identified in platelets, was isolated and identified from an extract of ovine articular cartilage. Immunoreactive material from a cartilage extract comigrated on gel electrophoresis with purified human platelet thrombospondin. When articular chondrocytes were cultured in the presence of 35S-methionine, metabolically labeled thrombospondin was immunoprecipitated from the culture medium and cell layer extract. These results demonstrate that thrombospondin is present in articular cartilage and is synthesized by articular chondrocytes.
Miller
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RR
Department of Musculoskeletal Research, Cleveland Clinic Foundation Research Institute 44195.
McDevitt
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CA
D000818:Animals; D002358:Cartilage, Articular / Q000378:metabolism*; D002478:Cells, Cultured; D011232:Chemical Precipitation; D002846:Chromatography, Affinity; D006023:Glycoproteins / Q000378:metabolism*; D007163:Immunosorbent Techniques; D066298:In Vitro Techniques; D012756:Sheep; D019699:Thrombospondins
D006023:Glycoproteins; D019699:Thrombospondins