Inhibitory monoclonal antibody against a (myristylated) small-molecular-weight antigen from Plasmodium falciparum associated with the parasitophorous vacuole membrane.
A small-molecular-weight antigen that occurs in asexual blood stages in synchronized cultures of Plasmodium falciparum was detected by a monoclonal antibody which inhibits parasite growth in vitro. This antigen, QF116, showed a molecular weight of 15,000 in parasite strain FCR-3K+ from The Gambia and 19,000 in strain FCQ-27 from Papua New Guinea. The protein did not show significant glycosylation by galactose or glucosamine labeling but was found to be acylated by myristic acid. By using immunogold labeling and electron microscopy, the location of the antigen could be attributed to the parasitophorous vacuole membrane and to inclusions and vesicles residing within the cytoplasm of the erythrocyte host cell.
Kara
U A
UA
Queensland Institute of Medical Research, Brisbane, Australia.
Stenzel
D J
DJ
Ingram
L T
LT
Bushell
G R
GR
Lopez
J A
JA
Kidson
C
C
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000913:Antibodies, Protozoan / Q000276:immunology*; D000953:Antigens, Protozoan / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology*; D004912:Erythrocytes / Q000469:parasitology; D005455:Fluorescent Antibody Technique; D007150:Immunohistochemistry; D007163:Immunosorbent Techniques; D008565:Membrane Proteins / Q000276:immunology*; D019814:Myristic Acid; D009227:Myristic Acids / Q000378:metabolism*; D009500:Neutralization Tests; D010963:Plasmodium falciparum / Q000254:growth & development / Q000276:immunology*; D014617:Vacuoles / Q000276:immunology
D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D000954:Antigens, Surface; D008565:Membrane Proteins; D009227:Myristic Acids; D019814:Myristic Acid