The binding of FITC-insulin to ANAE-positive cells in rat thymus.
To determine if thymic macrophages have insulin receptors, alternate sections of rat thymus were stained with FITC-insulin and examined for nonspecific esterase (ANAE) activity. Cells showing a diffuse ANAE staining pattern also bound FITC-insulin. These cells were concentrated in the cortico-medullary border and increased in number following administration of cortisol. Thymic macrophages may be insulin-dependent and therefore could be malfunctional in diabetes.
Hofert
J F
JF
Department of Biochemistry, University of Nebraska College of Medicine, Omaha 68105.
Mahowald
T A
TA
Mahowald
N A
NA
Heidrick
M L
ML
D000818:Animals; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives*; D007328:Insulin / Q000031:analogs & derivatives* / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D009283:Naphthol AS D Esterase / Q000378:metabolism*; D051381:Rats; D011972:Receptor, Insulin / Q000378:metabolism*; D013950:Thymus Gland / Q000166:cytology*
D007328:Insulin; C031573:insulin, fluorescein-isothiocyanated-; D011972:Receptor, Insulin; D009283:Naphthol AS D Esterase; D016650:Fluorescein-5-isothiocyanate