Monoclonal antibody-based enzyme-linked immunosorbent assay (ELISA) for detection of Plasmodium malariae sporozoites in mosquitoes.
A monoclonal antibody specific for a repeated epitope of the circumsporozoite protein of Plasmodium malariae sporozoites has been used to develop a two-site, single antibody-based enzyme-linked immunosorbent assay that can detect P. malariae sporozoites in mosquitoes. The assay uses a purified monoclonal antibody produced against sporozoites of the Uganda I/CDC strain of P. malariae to capture the antigen and the same monoclonal antibody labeled with horseradish peroxidase as the detector. Sporozoites have been detected in laboratory-infected mosquitoes stored at room temperature in the presence of a desiccant for as long as 18 months. The detection limit of the assay is approximately 50 P. malariae sporozoites per test well. Cross-reaction has not been observed with mosquitoes infected with P. falciparum, P. vivax, or P. ovale sporozoites.
Collins
F H
FH
Malaria Branch, Center for Infectious Diseases, Atlanta, Georgia 30333.
Procell
P M
PM
Campbell
G H
GH
Collins
W E
WE
D000818:Animals; D000852:Anopheles / Q000276:immunology / Q000469:parasitology*; D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan / Q000032:analysis*; D000954:Antigens, Surface / Q000032:analysis* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D010965:Plasmodium malariae / Q000276:immunology / Q000302:isolation & purification*; D015800:Protozoan Proteins; D012469:Salivary Glands / Q000469:parasitology
D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan; D000954:Antigens, Surface; D015800:Protozoan Proteins; C041595:circumsporozoite protein, Protozoan