Optimizing electroporation parameters for a variety of human hematopoietic cell lines.
The parameters affecting electroporation of four human hematopoietic cell lines were investigated. The optimal conditions for electroporation are described for both transient and stable expression of foreign genes. A correlation exists between the levels of transient gene expression and stable transfection frequency. In addition, linear DNA yields higher stable transfection frequencies than supercoiled DNA. The cumulative results indicate that electroporation is a simple and useful method for obtaining transient and stable expression of foreign genes in human hematopoietic cells.
McNally
M A
MA
Div. of Molecular Biology, Applied ImmuneSciences, Inc., Menlo Park, CA 94025-1109.
Lebkowski
J S
JS
Okarma
T B
TB
Lerch
L B
LB
D002460:Cell Line; D004560:Electricity; D004566:Electrodes; D006412:Hematopoietic Stem Cells; D006801:Humans; D014162:Transfection / Q000235:genetics*