(NZW x BXSB)F1 mouse. A new animal model of idiopathic thrombocytopenic purpura.
A decrease in thrombocyte count was observed in (NZW x BXSB)F1 (W/B F1) mice at the age of greater than 5 mo, whereas megakaryocyte counts were found to increase in such mice. FACS analyses revealed the presence of both platelet-associated antibodies (PAA) and circulating antiplatelet antibodies. There is a correlation between the presence of these antibodies and the degree of thrombocytopenia. The transplantation of normal bone marrow cells from BALB/c nu/nu mice to W/B F1 mice was found to have preventative and curative effects on thrombocytopenia; the mice showed normal platelet counts and no evidence of circulating antiplatelet antibodies. These results indicate that thrombocytopenia in W/B F1 mice is due to the presence of antibodies to platelets. We therefore think that W/B F1 mice serve as a useful animal model of idiopathic thrombocytopenic purpura (ITP) not only for elucidating the mechanism of the development of antiplatelet antibodies, but also for characterizing autoantibodies to platelets.
Oyaizu
N
N
Department of Pathology, Kansai Medical University, Osaka, Japan.
Yasumizu
R
R
Miyama-Inaba
M
M
Nomura
S
S
Yoshida
H
H
Miyawaki
S
S
Shibata
Y
Y
Mitsuoka
S
S
Yasunaga
K
K
Morii
S
S
D000818:Animals; D001323:Autoantibodies / Q000276:immunology; D001792:Blood Platelets / Q000276:immunology; D016026:Bone Marrow Transplantation; D006579:Heterozygote; D051379:Mice; D008817:Mice, Mutant Strains; D010976:Platelet Count; D011696:Purpura, Thrombocytopenic / Q000503:physiopathology* / Q000628:therapy
D001323:Autoantibodies