title: Independent variations of myc amplification, inducibility, maturation, and proliferation states in HL60.
abstract: We have investigated the possible relationship between c-myc gene activity and other variable traits in HL60. In a panel of variant lines, a good correlation was observed between myc gene copy number and the level of myc mRNA. There was no correlation between myc amplification or expression and the resistance of the lines to induction of terminal neutrophilic or monocytic differentiation. Therefore, myc mRNA level does not appear to determine the ability of the variant HL60 lines to respond to inducers of differentiation. Flow cytometric analyses of the expression of a differentiation antigen (AGF 4.36) revealed stable negative and positive subpopulations in growing HL60. myc amplification, expression, and inducibility were identical in these subpopulations, suggesting that variation of these traits in HL60 sublines and variants is not due to maturation state differences. myc gene copy number was also identical in transferrin receptor positive (proliferating) and negative (resting) populations. These data contradict the notion that myc amplification has been important in determining the in vitro biological properties of HL60.
authors.affiliation: Department of Tumor Biology, Karolinska Institutet, Stockholm, Sweden.
authors.forename: G
authors.initials: G
authors.lastname: Szabó
authors.forename: C
authors.initials: C
authors.lastname: Bunce
authors.forename: G
authors.initials: G
authors.lastname: Brown
authors.forename: G
authors.initials: G
authors.lastname: Klein
authors.forename: J
authors.initials: J
authors.lastname: Sümegi
mesh_terms: D002455:Cell Division; D004247:DNA / Q000032:analysis; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D005784:Gene Amplification; D005786:Gene Expression Regulation; D006801:Humans; D015470:Leukemia, Myeloid, Acute; D009693:Nucleic Acid Hybridization; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D016271:Proto-Oncogene Proteins c-myc; D011519:Proto-Oncogenes; D012333:RNA, Messenger / Q000032:analysis; D014407:Tumor Cells, Cultured
chemical_list: D011518:Proto-Oncogene Proteins; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger; D004247:DNA