The cytolytic toxin aerolysin must aggregate to disrupt erythrocytes, and aggregation is stimulated by human glycophorin.
The hole-forming toxin aerolysin was shown to aggregate after binding to erythrocytes at 37 degrees C. Although the protein also bound and aggregated at 4 degrees C, hole formation was not observed, indicating that aggregation preceded penetration of the lipid bilayer. Aggregation, but not binding, could be blocked by pretreatment of the toxin with diethyl pyrocarbonate, a histidine-reactive reagent. This resulted in inactivation of the toxin. Incubation of aerolysin with glycophorin purified from human erythrocytes caused aggregation and complete inactivation. Erythrocytes which lacked glycophorin were less sensitive to the toxin. Proaerolysin, the inactive precursor of aerolysin, also bound to erythrocytes; however, it did not aggregate, nor did it aggregate when preincubated with glycophorin. The protoxin could be activated by treatment with trypsin even after it had bound to erythrocytes. Activation could also be achieved by reaction of proaerolysin with a variety of other proteases, each of which brought about a similar reduction in protein molecular weight. The activated protein was resistant to further proteolysis. These results indicate that aggregation is a necessary step in hole formation and that the sites on aerolysin required for binding and for aggregation and hole formation are separate.
Garland
W J
WJ
Department of Biochemistry and Microbiology, University of Victoria, British Columbia, Canada.
Buckley
J T
JT
D001427:Bacterial Toxins / Q000378:metabolism / Q000633:toxicity*; D055598:Chemical Phenomena; D002621:Chemistry; D004910:Erythrocyte Membrane / Q000502:physiology; D006021:Glycophorins / Q000502:physiology*; D006461:Hemolysis; D006801:Humans; D046911:Macromolecular Substances; D010447:Peptide Hydrolases / Q000494:pharmacology; D052899:Pore Forming Cytotoxic Proteins; D011498:Protein Precursors / Q000378:metabolism; D012795:Sialoglycoproteins / Q000502:physiology*; D013329:Structure-Activity Relationship; D013696:Temperature; D014357:Trypsin / Q000494:pharmacology
D001427:Bacterial Toxins; D006021:Glycophorins; D046911:Macromolecular Substances; D052899:Pore Forming Cytotoxic Proteins; D011498:Protein Precursors; D012795:Sialoglycoproteins; C032676:aerolysin; D010447:Peptide Hydrolases; D014357:Trypsin