Field inversion gel electrophoretic separation of Cryptosporidium spp. chromosome-sized DNA.
Chromosomal DNA from 5 isolates of Cryptosporidium parvum and 1 of C. baileyi were compared by field-inversion gel electrophoresis (FIGE). FIGE analyses of parasite DNA prepared from purified sporozoites versus intact oocysts showed no observable differences. Chromosomal DNA migration patterns of the 5 C. parvum isolates were indistinguishable, whereas similar but distinct differences were evident between C. baileyi and the isolates of C. parvum. Oocyst-reactive monoclonal antibodies differentiated oocysts of C. parvum from those of C. baileyi but were unable to distinguish oocysts of 1 isolate of C. parvum from another.
Mead
J R
JR
Department of Microbiology, University of Arizona, Tucson 85721.
Arrowood
M J
MJ
Current
W L
WL
Sterling
C R
CR
D000818:Animals; D002875:Chromosomes / Q000032:analysis*; D003044:Coccidia / Q000235:genetics*; D003458:Cryptosporidium / Q000145:classification / Q000235:genetics*; D004247:DNA / Q000032:analysis*; D004587:Electrophoresis, Agar Gel / Q000379:methods; D005455:Fluorescent Antibody Technique
D004247:DNA