[Immunodiagnosis of AIDS using preparations of the recombinant antigen of the surface protein of the HTLV-III virus].
A technological scheme has been developed for purification of recombinant antigen of surface protein (ASP) of the causative virus of AIDS from Escherichia coli cells carrying plasmid pL2 which codes for the synthesis of a hybrid polypeptide consisting of phage lambda N protein (59 amino acid residues) and a fragment of SP (env) of HIV virus (569 a.r.). Purification of ASP is based on two separation principles: fractionation of polypeptides of bacterial lysates by preparative isoelectrofocusing in a granulated gel layer and the method of preparative polyacrylamide gel electrophoresis in "Multiphor" apparatus (Pharmacia). The ASP purified by these methods was used for construction of an immunodiagnostic preparation for AIDS, employing for this purpose solid-phase enzyme-immunoassay in two modifications: competitive sandwich method and analysis of the tested sera with direct sorption of ASP on nitrocellulose filters.
Kovgan
A A
AA
Poltorak
A N
AN
Pasechnik
V A
VA
Zhdanov
V M
VM
D000163:Acquired Immunodeficiency Syndrome / Q000175:diagnosis*; D000914:Antibodies, Viral / Q000032:analysis; D000918:Antibody Specificity; D000954:Antigens, Surface / Q000276:immunology / Q000302:isolation & purification; D000956:Antigens, Viral / Q000276:immunology* / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D004926:Escherichia coli / Q000235:genetics / Q000276:immunology; D006678:HIV / Q000276:immunology*; D006801:Humans; D007159:Immunologic Tests / Q000295:instrumentation / Q000379:methods; D007525:Isoelectric Focusing / Q000379:methods; D010957:Plasmids; D011994:Recombinant Proteins / Q000276:immunology / Q000302:isolation & purification
D000914:Antibodies, Viral; D000954:Antigens, Surface; D000956:Antigens, Viral; D011994:Recombinant Proteins
Immunodiagnostika SPID s pomoshch'iu preparatov rekombinantnogo antigena belka poverkhnosti virusa HTLV-III.