Quantitative basic residue requirements in the cleavage-activation site of the fusion glycoprotein as a determinant of virulence for Newcastle disease virus.
Newcastle disease virus exhibits a wide range of pathogenicity and virulence which, as with all paramyxoviruses, is directly related to the cleavability of a precursor (F0) of the fusion glycoprotein by cellular proteases. Sequence analyses of the cleavage site of several virulent and avirulent isolates of the Newcastle disease virus serotype reveal a correlation between virulence or pathogenicity and a high content of basic amino acid residues at the cleavage site. A similar correlation has been seen for other paramyxoviruses.
Glickman
R L
RL
Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester 01605.
Syddall
R J
RJ
Iorio
R M
RM
Sheehan
J P
JP
Bratt
M A
MA
D000595:Amino Acid Sequence; D009522:Newcastle disease virus / Q000378:metabolism / Q000472:pathogenicity*; D010447:Peptide Hydrolases / Q000378:metabolism; D011498:Protein Precursors / Q000378:metabolism; D013329:Structure-Activity Relationship; D014760:Viral Fusion Proteins / Q000378:metabolism*
D011498:Protein Precursors; D014760:Viral Fusion Proteins; D010447:Peptide Hydrolases