title: Intermolecular complexes between three human CD1 molecules on normal thymus cells.
abstract: The first cluster of differentiation (CD1) defines at least three distinct human thymic cell-surface differentiation antigens-CD1a, CD1b, and CD1c. We looked for structural homology of the three CD1 heavy chains at their peptide level by two-dimensional peptide maps. We show here that the CD1a Mr 49,000 heavy chain and the CD1b Mr 45,000 heavy chain appear to be more homologous to each other than to the CD1c Mr 43,000 heavy chain and that only one tyrosil peptide is common to the three heavy chains. Study of the CD1 heavy chains from several individuals reveals a very limited polymorphism of these molecules. We also demonstrate here that CD1a or CD1a-like molecules and other CD1 molecules can form intermolecular complexes on the surface of normal thymus cells. Molecules that are structurally very similar to CD1a molecules are associated noncovalently either with CD1c molecules or with CD1b molecules, and only CD1a molecules can associate covalently with CD8 molecules. In contrast, we could not find these intermolecular complexes on the surface of leukemic T-cell lines in culture.
authors.affiliation: INSERM U93, Institut de Recherche sur les Maladies du Sang, Paris, France.
authors.forename: M
authors.initials: M
authors.lastname: Amiot
authors.forename: H
authors.initials: H
authors.lastname: Dastot
authors.forename: M
authors.initials: M
authors.lastname: Fabbi
authors.forename: L
authors.initials: L
authors.lastname: Degos
authors.forename: A
authors.initials: A
authors.lastname: Bernard
authors.forename: L
authors.initials: L
authors.lastname: Boumsell
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D000945:Antigens, Differentiation, T-Lymphocyte / Q000302:isolation & purification*; D002460:Cell Line; D002648:Child; D006801:Humans; D007942:Leukemia, Experimental / Q000276:immunology; D046911:Macromolecular Substances; D008970:Molecular Weight; D010449:Peptide Mapping; D011110:Polymorphism, Genetic; D011233:Precipitin Tests; D013601:T-Lymphocytes / Q000032:analysis* / Q000276:immunology / Q000473:pathology; D013950:Thymus Gland / Q000166:cytology*; D001613:beta 2-Microglobulin / Q000302:isolation & purification*
chemical_list: D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D046911:Macromolecular Substances; D001613:beta 2-Microglobulin