Mutagenic and clastogenic activity of direct-acting components from air pollutants of the Silesian industrial region.
Sequential elution solvent chromatography (SESC) developed by Farcasiu for characterization of coal liquids was used for the fractionation of benzene extracts of airborne particulate pollutants. Mutagenic and clastogenic activity of SESC fractions was determined by the Salmonella/microsome test and the assay for V79 cell chromosomal aberrations (CAs), respectively. Five out of 8 obtained fractions showed differentiated, direct and indirect mutagenic activity. Selected 'direct' fractions, examined by the rodent cell chromosome aberration test, also gave a clastogenic response that increased with prolonged treatment time. The SESC system combined with 2 biological assays, the Ames test and the CAs test, seems to be a useful method for examination of genotoxic components of environmental pollutants.
Motykiewicz
G
G
Department of Tumor Biology, Institute of Oncology, Gliwice, Poland.
Michalska
J
J
Szeliga
J
J
Cimander
B
B
D000393:Air Pollutants / Q000032:analysis / Q000494:pharmacology*; D000818:Animals; D005591:Chemical Fractionation; D002869:Chromosome Aberrations; D006224:Cricetinae; D005347:Fibroblasts / Q000187:drug effects; D007221:Industry; D008168:Lung; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism; D009152:Mutagenicity Tests; D011044:Poland; D051381:Rats; D012486:Salmonella typhimurium / Q000187:drug effects*
D000393:Air Pollutants