Pertussis toxin treatment attenuates some effects of insulin in BC3H-1 murine myocytes.
The effects of pertussis toxin (PT) treatment on insulin-stimulated myristoyl-diacylglycerol (DAG) generation, hexose transport, and thymidine incorporation were studied in differentiated BC3H-1 myocytes. Insulin treatment caused a biphasic increase in myristoyl-DAG production which was abolished in myocytes treated with PT. There was no effect of PT treatment on basal (nonstimulated) myristoyl-DAG production. Insulin-stimulated hydrolysis of a membrane phosphatidylinositol glycan was blocked by PT treatment. ADP-ribosylation of BC3H-1 plasma membranes with [32P]NAD revealed a 40-kDa protein as the major PT substrate in vivo and in vitro. The time course and dose dependence of the effects of PT on diacylglycerol generation correlated with the in vivo ADP-ribosylation of the 40-kDa substrate. Pertussis toxin treatment resulted in a 71% attenuation of insulin-stimulated hexose uptake without effect on either basal or phorbol ester-stimulated uptake. The stimulatory effects of insulin and fetal calf serum on [3H]thymidine incorporation into quiescent myocytes were attenuated by 61 and 59%, respectively, when PT was added coincidently with the growth factors. Nonstimulated and EGF-stimulated [3H]thymidine incorporation was unaffected by PT treatment. These data suggest that a PT-sensitive G protein is involved in the cellular signaling mechanisms of insulin.
Luttrell
L M
LM
Department of Pediatrics, University of Virginia School of Medicine, Charlottesville 22908.
Hewlett
E L
EL
Romero
G
G
Rogol
A D
AD
D000246:Adenosine Diphosphate Ribose / Q000378:metabolism; D000818:Animals; D002855:Chromatography, Thin Layer; D004075:Diglycerides / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005944:Glucosamine / Q000378:metabolism; D007294:Inositol / Q000378:metabolism; D007328:Insulin / Q000494:pharmacology*; D051379:Mice; D008970:Molecular Weight; D009132:Muscles / Q000187:drug effects* / Q000378:metabolism; D009226:Myristates / Q000494:pharmacology; D009243:NAD / Q000378:metabolism; D037342:Pertussis Toxin; D010566:Virulence Factors, Bordetella / Q000494:pharmacology*
D004075:Diglycerides; D007328:Insulin; D009226:Myristates; D010566:Virulence Factors, Bordetella; D009243:NAD; D000246:Adenosine Diphosphate Ribose; D007294:Inositol; D037342:Pertussis Toxin; D005944:Glucosamine