title: Construction of a fol mutant strain of Escherichia coli for use in dihydrofolate reductase mutagenesis experiments.
abstract: A strain of Escherichia coli with the fol gene deleted and a kan gene inserted in its place was created for use in cloning and isolation of mutant dihydrofolate reductase. Southern blot analysis and dihydrofolate reductase enzyme assays confirmed the delta fol::kan genotype. A thyA mutation accompanied the fol deletion and is required for survival of a dihydrofolate reductase-deficient strain.
authors.affiliation: Department of Biochemistry, Washington University Medical School, St. Louis, Missouri 63110.
authors.forename: P M
authors.initials: PM
authors.lastname: Ahrweiler
authors.forename: C
authors.initials: C
authors.lastname: Frieden
mesh_terms: D002872:Chromosome Deletion; D003001:Cloning, Molecular; D004352:Drug Resistance, Microbial / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D007612:Kanamycin / Q000494:pharmacology; D009154:Mutation; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics* / Q000302:isolation & purification; D013940:Thymidylate Synthase / Q000235:genetics; D014161:Transduction, Genetic; D014169:Transformation, Bacterial
chemical_list: D007612:Kanamycin; D013762:Tetrahydrofolate Dehydrogenase; D013940:Thymidylate Synthase