[Physico-chemical and immunochemical characteristics of oncoprecipitins of crustacin and cyprein specifically reacting with carcinoembryonic antigen].
Crustacin from Pagurus prideauxii and cyprein from Cyprea caputserpentis have been shown to be glycoproteins with molecular masses 36 +/- 1 and 44 +/- 1.4 kDa, containing 3 and 18% of carbohydrates, respectively. Their amino acid and monosaccharide composition have been determined. Crustacin or cyprein interact with CEA more specifically than carbohydrate-containing polymers with lectins. Carbohydrates unspecifically inhibit this interaction at rather high concentrations. Association constants of CEA-crustacin and CEA-cyprein complexes are 0.6.10(8) M-1, respectively. Besides, ELISA showed that antibodies against CEA bind antibodies to crustacin and cyprein.
Pavlenko
A F
AF
Kurika
A V
AV
Chikalovets
I V
IV
Belogortseva
N I
NI
Ovodov
Iu S
IuS
D000818:Animals; D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D002272:Carcinoembryonic Antigen / Q000276:immunology* / Q000302:isolation & purification; D002471:Cell Transformation, Neoplastic / Q000276:immunology*; D003445:Crustacea; D004591:Electrophoresis, Polyacrylamide Gel; D007122:Immunoelectrophoresis; D007124:Immunoenzyme Techniques; D011233:Precipitin Tests; D011234:Precipitins / Q000276:immunology* / Q000302:isolation & purification
D002272:Carcinoembryonic Antigen; D011234:Precipitins
Fiziko-khimicheskaia i immunokhimicheskaia kharakteristika onkopretsipitinov krustatsina i tsipreina, spetsificheski reagiruiushchikh s rakovoémbrional'nym antigenom.