Modulation of mouse spleen natural killer (NK) cell activity by beta-interferon, interleukin-1, and prostaglandins.
By using CBA/J mice as a source of effector cells and Yac-1 lymphoma line as "target" cells, the natural killer (NK) cell activity was assayed following both in vivo and in vitro immunomodulation [beta-interferon (IF), interleukin-1 (IL-1), indomethacin (IND), prostaglandin-E2 (PGE)]. Only IF/IND and IL-1/IND mixed in vivo led to a significant augmentation of NK cell activity. If exposed in vitro to IF or to IL-1, control group-derived spleen NK cells exhibited increased cytotoxic activity whereas PGE-exposure only was followed by a lower cytotoxic level as expressed both in % cytotoxicity curves and in lytic units 20%/10(7) effector cells. On the other hand, PGE seemed to activate the "nonspecific suppressor" (NSS) cell subset in its inhibitory effect about NK cells as tested in vitro in several NK/NSS cell mixtures at different ratios. IF, but not IL-1 diminished the NSS-cell-induced suppressive activity. Pre-exposure of NK/NSS cell mixtures to IF followed by PGE exposure, did not prevent PGE-dependent NSS cell activation.
Voiculescu
C L
CL
Department of Microbiology, Faculty of Medicine, Craiova, Rumania.
Rosu
L
L
Rogoz
S
S
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D003602:Cytotoxicity, Immunologic; D015232:Dinoprostone / Q000494:pharmacology*; D007213:Indomethacin / Q000494:pharmacology*; D007370:Interferon Type I / Q000494:pharmacology*; D007375:Interleukin-1 / Q000494:pharmacology*; D007694:Killer Cells, Natural / Q000276:immunology*; D051379:Mice; D008808:Mice, Inbred CBA; D013154:Spleen / Q000276:immunology
D000276:Adjuvants, Immunologic; D007370:Interferon Type I; D007375:Interleukin-1; D015232:Dinoprostone; D007213:Indomethacin