Human hybridoma suppressor factor acts selectively on CD4+ cells.
A human thymus cell hybridoma secretes an immunosuppressive lymphokine, referred to as hybridoma suppressor factor (HSF). This factor suppresses polyclonal immunoglobulin (Ig) production as well as Interleukin-2 (IL-2) production in pokeweed mitogen (PWM)-stimulated peripheral blood mononuclear cells (PBMC). Similar suppression of Ig and IL-2 production was observed in reconstituted cultures of CD4+ cells and non-T cells. Here we analysed, further, the mechanism of HSF-mediated suppression of Ig and IL-2 production. We demonstrated that HSF inhibited PWM-induced IL-2 production by CD4+ cells but not by CD8+ cells and its suppressive activity on Ig production was totally abrogated by preabsorption with CD4+ cells, but not by CD8+ cells. These results indicate a subset specific action of HSF.
Tomita
Y
Y
Department of Immunology and Cancer, Research Institute, Cleveland Clinic Foundation, OH 44106.
Dustoor
M M
MM
Cathcart
M K
MK
D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis*; D006801:Humans; D006825:Hybridomas / Q000276:immunology*; D007136:Immunoglobulins / Q000096:biosynthesis; D007376:Interleukin-2 / Q000096:biosynthesis; D007963:Leukocytes, Mononuclear / Q000276:immunology; D013491:Suppressor Factors, Immunologic / Q000378:metabolism*; D013601:T-Lymphocytes / Q000276:immunology*
D000945:Antigens, Differentiation, T-Lymphocyte; D007136:Immunoglobulins; D007376:Interleukin-2; D013491:Suppressor Factors, Immunologic