title: Modulation of folding pathways of exported proteins by the leader sequence.
abstract: Leader peptides that function to direct export of proteins through membranes have some common features but exhibit a remarkable sequence diversity. Thus there is some question whether leader peptides exert their function through conventional stereospecific protein-protein interaction. Here it is shown that the leader peptides retarded the folding of precursor maltose-binding protein and ribose-binding protein from Escherichia coli. This kinetic effect may be crucial in allowing precursors to enter the export pathway.
authors.affiliation: Biochemistry/Biophysics Program, Washington State University, Pullman 99164.
authors.forename: S
authors.initials: S
authors.lastname: Park
authors.forename: G
authors.initials: G
authors.lastname: Liu
authors.forename: T B
authors.initials: TB
authors.lastname: Topping
authors.forename: W H
authors.initials: WH
authors.lastname: Cover
authors.forename: L L
authors.initials: LL
authors.lastname: Randall
mesh_terms: D018528:ATP-Binding Cassette Transporters; D001426:Bacterial Proteins / Q000378:metabolism*; D001692:Biological Transport; D002352:Carrier Proteins / Q000378:metabolism; D019286:Endopeptidase K; D004926:Escherichia coli / Q000378:metabolism*; D029968:Escherichia coli Proteins; D019791:Guanidine; D006146:Guanidines / Q000494:pharmacology; D007700:Kinetics; D058619:Maltose-Binding Proteins; D009004:Monosaccharide Transport Proteins; D010447:Peptide Hydrolases; D033902:Periplasmic Binding Proteins; D011487:Protein Conformation / Q000187:drug effects; D011489:Protein Denaturation; D011498:Protein Precursors / Q000378:metabolism*; D021382:Protein Sorting Signals / Q000494:pharmacology / Q000502:physiology*; D012697:Serine Endopeptidases / Q000494:pharmacology; D013050:Spectrometry, Fluorescence
chemical_list: D018528:ATP-Binding Cassette Transporters; D001426:Bacterial Proteins; D002352:Carrier Proteins; D029968:Escherichia coli Proteins; D006146:Guanidines; D058619:Maltose-Binding Proteins; D009004:Monosaccharide Transport Proteins; D033902:Periplasmic Binding Proteins; D011498:Protein Precursors; D021382:Protein Sorting Signals; C028003:RbsB protein, E coli; C461599:maltose transport system, E coli; D010447:Peptide Hydrolases; D012697:Serine Endopeptidases; D019286:Endopeptidase K; D019791:Guanidine