Separation and detection of DNA by capillary electrophoresis.
The use of capillary electrophoresis for the separation and detection of nucleic acids has been investigated. Lab-model instruments have been built, using commercially available UV absorbance and fluorescence detectors which were modified for use with 50-100 microns I.D. fused-silica capillary tubing. The sensitivity of these instruments (signal-to-noise ratio = 3) was measured as 15 micrograms/ml for fluorescence detection of ethidium bromide-stained herring-sperm DNA and 3 micrograms/ml for UV absorbance detection. With the former instruments a variety of strategies has been used to attain rapid separations of bases, oligonucleotides, restriction fragments and whole phage, viral and plasmid DNAs.
Kasper
T J
TJ
Microphoretic Systems, Inc., Sunnyvale, CA 94086.
Melera
M
M
Gozel
P
P
Brownlee
R G
RG
D004247:DNA / Q000032:analysis*; D015252:Deoxyribonucleases, Type II Site-Specific; D004586:Electrophoresis; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
D004247:DNA; D015252:Deoxyribonucleases, Type II Site-Specific; C024403:GGCC-specific type II deoxyribonucleases