Role of pokeweed mitogen spleen conditioned medium in regulating haematopoiesis in long-term human marrow cultures.
The modulatory effect of pokeweed mitogen spleen conditioned medium (PWM-SCM) on the growth of myeloid progenitors (CFU-C) and on the composition of the stromal layer in human bone marrow long-term cultures was studied. In the presence of PWM-SCM there was enhanced CFU-C growth in the adherent layer throughout 6 weeks of incubation, with 2-fold, 2-fold and 3.4-fold increases during wk 2, 4 and 6 respectively (p less than 0.05). In the non-adherent layer of control cultures, however, there was a rapid decline in haematopoiesis which could not be offset by PWM-SCM. The differentiation pattern of CFU-C (entailing monocyte - macrophage progenitors CFU-M, granulocytic progenitors CFU-G or monocyte - granulocyte progenitors CFU-GM) was not altered by PWM-SCM. Fewer lipid-containing cells developed in the adherent layer of cultures treated with PWM-SCM than in control cultures, but the distribution pattern of monocytes - macrophages or granulocytes was not affected. These results suggest that in long-term cultures of human marrow cells there may be extensive haematopoiesis in the presence of PWM-SCM. Whether this is a direct effect on progenitor cells of stimulating factors, or rather of accessory cells in the adherent layer, remains to be clarified.
Williams
Z
Z
Department of Histology and Cell Biology, Sackler Faculty of Medicine, Ramat Aviv, Israel.
Fabian
I
I
D001854:Bone Marrow Cells; D002448:Cell Adhesion; D002470:Cell Survival; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D003470:Culture Media / Q000494:pharmacology; D006133:Growth Substances / Q000502:physiology*; D016298:Hematopoietic Cell Growth Factors; D006412:Hematopoietic Stem Cells / Q000145:classification / Q000502:physiology*; D006801:Humans; D007958:Leukocyte Count; D011043:Pokeweed Mitogens / Q000494:pharmacology; D013154:Spleen
D003470:Culture Media; D006133:Growth Substances; D016298:Hematopoietic Cell Growth Factors; D011043:Pokeweed Mitogens