title: Regulation of pyruvate kinase in Reuber H35 hepatoma cells by insulin and fructose.
abstract: 1. Kinetic and immunological studies as well as electrophoretic behaviour indicated that pyruvate kinase in Reuber H35 hepatoma cells is of the M2-type. 2. Addition of 0.1 microM insulin or 2 mM fructose to the incubation medium for 72 hr increased the activity of the M2-type pyruvate kinase in Reuber H35 hepatoma cells by 103 and 25% respectively. 3. Incorporation studies with [3H]leucine followed by immunoprecipitation showed that the apparent rate of synthesis of the M2-type pyruvate kinase was increased by both insulin and fructose. 4. Degradation studies indicated that the addition of insulin and fructose to the incubation medium increased the half-life of the M2-type pyruvate kinase from 4.8 to 8.6 and 6.8 hr respectively.
authors.affiliation: Department of Biochemistry, University of Hong Kong.
authors.forename: S S
authors.initials: SS
authors.lastname: Wong
authors.forename: S W
authors.initials: SW
authors.lastname: Wu
authors.forename: D C
authors.initials: DC
authors.lastname: Yeung
mesh_terms: D000818:Animals; D004790:Enzyme Induction / Q000187:drug effects; D005632:Fructose / Q000494:pharmacology*; D006207:Half-Life; D007163:Immunosorbent Techniques; D007328:Insulin / Q000494:pharmacology*; D007527:Isoenzymes / Q000096:biosynthesis*; D008114:Liver Neoplasms, Experimental / Q000201:enzymology*; D011770:Pyruvate Kinase / Q000096:biosynthesis / Q000378:metabolism*; D051381:Rats; D014407:Tumor Cells, Cultured
chemical_list: D007328:Insulin; D007527:Isoenzymes; D005632:Fructose; D011770:Pyruvate Kinase