title: Application of the molecular replacement method to multidomain proteins. 1. Determination of the orientation of an immunoglobulin Fab fragment.
abstract: Multidomain proteins provide special problems in the application of the molecular replacement method of structure determination. The structure of the Fab fragment from the autoimmune poly(dT)-specific antibody HED10 has been determined using molecular replacement. An analysis of the effects of varying the model and the parameters used in the rotation function indicates that dividing the molecule into individual relatively rigid domains simplifies interpretation of the results, and that the optimal parameters depend on the molecule under study.
authors.affiliation: Department of Biochemistry, University of Alberta, Edmonton, Canada.
authors.forename: M
authors.initials: M
authors.lastname: Cygler
authors.forename: W F
authors.initials: WF
authors.lastname: Anderson
mesh_terms: D000906:Antibodies; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D007140:Immunoglobulin Fab Fragments; D008956:Models, Chemical; D015394:Molecular Structure; D011071:Poly T / Q000276:immunology
chemical_list: D000906:Antibodies; D007140:Immunoglobulin Fab Fragments; D011071:Poly T