title: Influence of epidermal growth factor on growth of bovine mammary tissue in athymic nude mice.
abstract: Bovine mammary tissue obtained from midpregnant Holstein heifers by surgical biopsy was transplanted subcutaneously to ovariectomized athymic nude mice (n = 5 heifers). After 3 weeks recovery, mice were either sham operated or sialoadenectomized (submandibular salivary glands removed). After an additional week, mice were injected with saline or 17 beta-estradiol + progesterone (1 microgram + 1 mg/day) for 2 days. In addition, half of the sialoadenectomized mice were injected with epidermal growth factor (5 micrograms/day). Grafted tissue was removed and rate of deoxyribonucleic acid (DNA) synthesis estimated by incorporation of 3H thymidine. Estradiol + progesterone increased the incorporation of 3H thymidine from 77 +/- 20 dpm/micrograms DNA to 472 +/- 53 dpm/micrograms DNA. In sialoadenectomized mice, DNA synthesis was increased from 88 +/- 16 dpm/micrograms DNA (saline treated) to 360 +/- 29 dpm/micrograms DNA (estradiol + progesterone treated). In sialoadenectomized mice treated with epidermal growth factor, DNA synthesis in estradiol + progesterone treated mice was 529 +/- 36 dpm/micrograms DNA, compared to 112 +/- 30 dpm/micrograms DNA in sialoadenectomized mice treated with epidermal growth factor. These data indicate that sialoadenectomy of athymic nude mice decreased the ability of transplanted bovine mammary tissue to increase DNA synthesis in response to estradiol and progesterone. This inhibition was removed by epidermal growth factor treatment. These data suggest a physiological role of epidermal growth factor in regulating development and hormone responsiveness of bovine mammary tissue.
authors.affiliation: Dairy Science Department, University of Wisconsin, Madison 53706.
authors.forename: L G
authors.initials: LG
authors.lastname: Sheffield
authors.forename: I S
authors.initials: IS
authors.lastname: Yuh
mesh_terms: D000818:Animals; D002417:Cattle; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D005260:Female; D008321:Mammary Glands, Animal / Q000033:anatomy & histology / Q000187:drug effects / Q000254:growth & development / Q000637:transplantation*; D051379:Mice; D008819:Mice, Nude; D011247:Pregnancy; D014183:Transplantation, Heterologous
chemical_list: D004815:Epidermal Growth Factor