Role of proteinkinase C and phosphatidylinositol metabolism in lipopeptide-induced leukocyte activation as signal transducing mechanism.
Synthetic lipopeptides are potent B-lymphocyte and macrophage activators. The role of phosphatidylinositol metabolism and proteinkinase C in lipopeptide induced leukocyte activation were investigated. In murine B-lymphocytes and in bone marrow derived macrophages, lipopeptide failed to induce phosphatidylinositol breakdown, whereas in the macrophage cell line P388D1 formation of inositolphosphates was increased. Translocation of proteinkinase C from a cytosolic to a membrane compartment was only observed in the cell line P388D1 indicating that in the other cells tested lipopeptide acts via different signal transduction pathways.
Hauschildt
S
S
Institut für Immunbiologie der Universität Freiburg, F.R.G.
Steffens
U
U
Wagner-Roos
L
L
Bessler
W G
WG
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D001426:Bacterial Proteins / Q000494:pharmacology; D002460:Cell Line; D007962:Leukocytes / Q000276:immunology* / Q000378:metabolism; D008264:Macrophages / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010455:Peptides / Q000494:pharmacology*; D010716:Phosphatidylinositols / Q000378:metabolism*; D011493:Protein Kinase C / Q000378:metabolism*; D015398:Signal Transduction
D001426:Bacterial Proteins; D010455:Peptides; D010716:Phosphatidylinositols; D011493:Protein Kinase C