title: Use of immunosorbent-purified antigens of Fasciola hepatica in enzyme immunoassays.
abstract: A monoclonal antibody specific for the T1 tegumental antigen of Fasciola hepatica was used as a solid-phase immunosorbent for the purification of T1 antigen from homogenised mature F hepatica. Material fractionated by this technique was successfully used in enzyme-linked immunoassays to detect antibodies to F hepatica in sera from sheep and cattle. Species differences in response to infection by F hepatica were demonstrated.
authors.affiliation: Department of Biology, Queen's University of Belfast, Northern Ireland.
authors.forename: A
authors.initials: A
authors.lastname: Trudgett
authors.forename: A
authors.initials: A
authors.lastname: Anderson
authors.forename: R E
authors.initials: RE
authors.lastname: Hanna
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000947:Antigens, Helminth / Q000276:immunology* / Q000302:isolation & purification; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis*; D004797:Enzyme-Linked Immunosorbent Assay; D005210:Fasciola hepatica / Q000276:immunology*; D005211:Fascioliasis / Q000175:diagnosis / Q000662:veterinary*; D007163:Immunosorbent Techniques; D012756:Sheep; D012757:Sheep Diseases / Q000175:diagnosis*
chemical_list: D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth