title: Immunoperoxidase localization of apolipoprotein D in human enterocytes and hepatocytes.
abstract: In this study we prepared a pure apolipoprotein D and obtained a specific antiserum to it. The purified apolipoprotein D migrated as a single band of Mr = 29,000 but appeared as five isoforms on isoelectrofocusing. The antiserum did not cross-react with other apolipoproteins. Immunoenzymatic staining revealed the presence of apolipoprotein D in the perinuclear area of the cytoplasm of isolated normal hepatocytes and HepG2 cells. Apolipoprotein D was also localized in intestinal epithelium and in liver cells. The intracellular distribution of apolipoprotein D was similar to that of apolipoprotein B. Our results indicated that apolipoprotein D, like many other circulating apolipoproteins, is synthesized in enterocytes and hepatocytes.
authors.affiliation: INSERM Unité 9, Hôpital Saint-Antoine, Paris, France.
authors.forename: M E
authors.initials: ME
authors.lastname: Bouma
authors.forename: J P
authors.initials: JP
authors.lastname: de Bandt
authors.forename: M
authors.initials: M
authors.lastname: Ayrault-Jarrier
authors.forename: J
authors.initials: J
authors.lastname: Burdin
authors.forename: N
authors.initials: N
authors.lastname: Verthier
authors.forename: A
authors.initials: A
authors.lastname: Raisonnier
mesh_terms: D000328:Adult; D001053:Apolipoproteins / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D053399:Apolipoproteins D; D006801:Humans; D007106:Immune Sera; D007124:Immunoenzyme Techniques; D007413:Intestinal Mucosa / Q000166:cytology / Q000378:metabolism*; D008099:Liver / Q000166:cytology / Q000378:metabolism*
chemical_list: D001053:Apolipoproteins; D053399:Apolipoproteins D; D007106:Immune Sera