Induction of B-cell lymphomas in athymic NIH Swiss nu/nu mice.
A B-cell lymphoma was induced in athymic NIH Swiss nu/nu mice by challenging the animals with NIH3T3 cells, previously transfected with a recombinant DNA carrying a human oncogene hhcM, ligated to an SV40 promoter with a neomycin-resistance marker. The gross pathology of the tumor-bearing animal revealed generalized lymphadenopathy and the histopathology indicated widespread infiltration of lymphocytes into organs, such as brain, liver, kidney and lung, in addition to the lymphoid tissues and spleen; the appearance was consistent with diffuse histiocytic lymphoma. Direct immunofluorescence assays with specific typing anti-sera on live cells prepared from spleen and various lymph nodes in short-term culture, suggested the cells were B-cells. This B-cell lymphoma provides an experimental model, not only for studying possible oncogene activation but also for studying the various interactions involved in signal transduction essential for the activation of B-cell proliferation and differentiation.
Yang
S S
SS
Laboratory of Cellular Oncology, National Cancer Institute, Bethesda, MD 20892.
Vieira
W
W
Farshid
M
M
Cannon
G
G
Wivel
N A
NA
D000818:Animals; D001402:B-Lymphocytes / Q000473:pathology; D004273:DNA, Neoplasm / Q000235:genetics*; D004274:DNA, Recombinant; D008223:Lymphoma / Q000209:etiology* / Q000235:genetics / Q000473:pathology; D051379:Mice; D008819:Mice, Nude; D009857:Oncogenes; D014162:Transfection; D014407:Tumor Cells, Cultured / Q000637:transplantation
D004273:DNA, Neoplasm; D004274:DNA, Recombinant