title: Immunobiological properties of lipopolysaccharides isolated from Fusobacterium nucleatum and F. necrophorum.
abstract: Lipopolysaccharides (LPSs) were isolated from Fusobacterium nucleatum ATCC 10953 and F. necrophorum ATCC 25286 by the hot phenol/water procedure. F. nucleatum LPS was composed of 16% (w/w) carbohydrate, 10% (w/w) hexosamine and 40% (w/w) fatty acid, while F. necrophorum LPS was composed of 26% (w/w) carbohydrate, 12% (w/w) hexosamine and 28% (w/w) fatty acid. These LPS preparations induced mitogenic responses in spleen cells of BALB/c, BALB/c (nu/nu) and C3H/HeN mice, and these responses were suppressed by the addition of polymyxin B. The preparations also induced the polyclonal responses of C3H/HeN spleen cells. In addition, enhanced glucose utilization and interleukin-1 production by murine peritoneal macrophages were demonstrated. Neither spleen cells nor macrophages from the 'LPS-nonresponsive' C3H/HeJ mouse were activated by LPSs from the Fusobacterium species.
authors.affiliation: Department of Dental Research, National Institute of Health, Tokyo, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Okahashi
authors.forename: T
authors.initials: T
authors.lastname: Koga
authors.forename: T
authors.initials: T
authors.lastname: Nishihara
authors.forename: T
authors.initials: T
authors.lastname: Fujiwara
authors.forename: S
authors.initials: S
authors.lastname: Hamada
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D005673:Fusobacterium / Q000032:analysis*; D005675:Fusobacterium necrophorum / Q000032:analysis; D008070:Lipopolysaccharides / Q000032:analysis / Q000276:immunology*; D008213:Lymphocyte Activation; D008262:Macrophage Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H
chemical_list: D008070:Lipopolysaccharides