2-deoxy-D-glucose modulation of T-lymphocyte reactivity: differential effects on lymphoid compartments.
This study was designed to evaluate the effect of glucoprivation, as induced by 2-deoxy-D-glucose (2-DG) administration, on lymphocyte mitogen reactivity in Sprague-Dawley rats. The results showed that a single injection of 2-DG decreased reactivity in both whole-blood and spleen lymphocytes, as determined by mitogenic stimulation to concanavalin A (Con A) and phytohemagglutinin (PHA). However, the suppressed reactivity for the spleen lymphocytes attenuated with repeated injections, but the whole-blood lymphocytes did not show attenuation. Mitogen assessments of lymphocytes obtained from the thymus indicated that a single injection did not induce suppressed reactivity, but repeated injections induced a pronounced suppression of responsiveness. Furthermore, mitogen assessments of mesentery lymph nodes did not show any effect of 2-DG injections. These results corroborate other findings using electric shock as the stressor, namely that different compartments of the immune system are differentially affected by a stressor.
Lysle
D T
DT
Department of Pathology, University of Pittsburgh, Pennsylvania 15213.
Cunnick
J E
JE
Wu
R
R
Caggiula
A R
AR
Wood
P G
PG
Rabin
B S
BS
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D003837:Deoxy Sugars / Q000494:pharmacology*; D003847:Deoxyglucose / Q000494:pharmacology*; D007958:Leukocyte Count / Q000187:drug effects; D008198:Lymph Nodes / Q000187:drug effects / Q000276:immunology; D008297:Male; D008934:Mitogens / Q000494:pharmacology*; D009928:Organ Specificity; D051381:Rats; D011919:Rats, Inbred Strains; D013154:Spleen / Q000187:drug effects / Q000276:immunology; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology; D013950:Thymus Gland / Q000187:drug effects / Q000276:immunology
D001786:Blood Glucose; D003837:Deoxy Sugars; D008934:Mitogens; D003847:Deoxyglucose