title: Evolution and mutagenesis of the mammalian excision repair gene ERCC-1.
abstract: The human DNA excision repair protein ERCC-1 exhibits homology to the yeast RAD10 repair protein and its longer C-terminus displays similarity to parts of the E. coli repair proteins uvrA and uvrC. To study the evolution of this 'mosaic' ERCC-1 gene we have isolated the mouse homologue. Mouse ERCC-1 harbors the same pattern of homology with RAD10 and has a comparable C-terminal extension as its human equivalent. Mutation studies show that the strongly conserved C-terminus is essential in contrast to the less conserved N-terminus which is even dispensible. The mouse ERCC-1 amino acid sequence is compatible with a previously postulated nuclear location signal and DNA-binding domain. The ERCC-1 promoter harbors a region which is highly conserved in mouse and man. Since the ERCC-1 promoter is devoid of all classical promoter elements this region may be responsible for the low constitutive level of expression in all mouse tissues and stages of embryogenesis examined.
authors.affiliation: Department of Cell Biology and Genetics, Erasmus University, Rotterdam, The Netherlands.
authors.forename: M
authors.initials: M
authors.lastname: van Duin
authors.forename: J
authors.initials: J
authors.lastname: van den Tol
authors.forename: P
authors.initials: P
authors.lastname: Warmerdam
authors.forename: H
authors.initials: H
authors.lastname: Odijk
authors.forename: D
authors.initials: D
authors.lastname: Meijer
authors.forename: A
authors.initials: A
authors.lastname: Westerveld
authors.forename: D
authors.initials: D
authors.lastname: Bootsma
authors.forename: J H
authors.initials: JH
authors.lastname: Hoeijmakers
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D002460:Cell Line; D003001:Cloning, Molecular; D004260:DNA Repair; D004261:DNA Replication; D004926:Escherichia coli / Q000235:genetics; D005796:Genes; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D011506:Proteins / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
chemical_list: D011506:Proteins