[Stability of normal, abnormal and recombinant proteins in Escherichia coli strains deficient for intracellular proteinase La--the product of the lon gene].
Escherichia coli Lon-mutants deficient in intracellular protease La have been isolated. The rate of degradation of normal cellular proteins was 1.5-2-fold lower in Lon-mutants as compared with that of the wild type strain. The rate of degradation of canavanine-containing abnormal proteins, as well as foreign proteins was significantly higher in E. coli than that of normal proteins. Lon-mutants possessed 2-2.5-fold lower rates of degradation of abnormal proteins as compared with Lon+-strains. The rate of degradation of human interferon alpha-2 was 10-fold higher in E. coli than that of abnormal proteins. B. amyloliquefaciens alpha-amylase degraded in E. coli with the rate comparable with that of abnormal proteins, since chloramphenicolacetyltransferase from Tn9 was stable in E. coli. The rate of degradation of interferon alpha-2 was 2-fold lower in Lon-mutants (half-life 23-26 min) than in the initial strain (11-12 min). Lon-mutants were effectively used as recipient strains for constructing strains-producers of several human alpha- and beta-interferons.
Ryzhavskaia
A S
AS
Kaĭdalova
N V
NV
Anukhin
Iu M
IuM
Kliachko
E V
EV
Livshits
V A
VA
D049069:ATP-Dependent Proteases; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002172:Canavanine; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D029968:Escherichia coli Proteins; D006360:Heat-Shock Proteins; D006868:Hydrolysis; D007370:Interferon Type I / Q000378:metabolism; D009154:Mutation; D049070:Protease La; D011994:Recombinant Proteins / Q000378:metabolism*; D012697:Serine Endopeptidases / Q000172:deficiency* / Q000235:genetics
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D006360:Heat-Shock Proteins; D007370:Interferon Type I; D011994:Recombinant Proteins; D002172:Canavanine; D049069:ATP-Dependent Proteases; D012697:Serine Endopeptidases; C483567:Lon protein, E coli; D049070:Protease La
Izuchenie stabil'nosti normal'nykh, anomal'nykh i genno-inzhenernykh belkov v shtammakh Escherichia coli, defitsitnykh po vnutrikletochnoĭ proteinaze La--produkte gena lon.