The developmental pathology of maternally derived Thp fetuses.
The Thair pin (Thp) mutation is a deletion of 5 centimorgans of chromosome 17 in the mouse. When the mutant chromosome is passed to the fetus through the female, the heterozygous fetuses (Thp/+) die in utero. If the chromosome is passed through the male, the heterozygotes are viable and display a short-tailed phenotype. These maternally derived mutant embryos provide an excellent model system to study the effects of an incomplete female genome on development. The results reported here describe the findings of a pathological study of the affected fetuses from day 14 of development to birth. These observations indicate that the maternally derived Thp fetuses die in utero of congestive heart failure. The mutant fetuses displayed an enlarged heart, primarily the right side, and other cardiovascular abnormalities including ventricular septal defects, aortic stenosis, pulmonary artery dilation, and dilation of the venous circulatory system. The fetuses also displayed abnormal accumulation of extrafetal fluid in the visceral yolk sac and amion, as well as massive subcutaneous edema and ascites. The Thp fetuses were often pale and anemic, and they showed a decreased number of red blood cells per unit volume of blood and an increase in circulating nucleated red blood cells. Defects in the development of the labyrinthine and spongiotrophoblast regions of the placenta were also observed. The pathogenesis of the defects is discussed.
Babiarz
B S
BS
Department of Biological Sciences, Rutgers University, Piscataway, New Jersey 08855-1059.
Donovan
M J
MJ
Hathaway
H J
HJ
D000818:Animals; D001835:Body Weight; D002872:Chromosome Deletion; D000013:Congenital Abnormalities / Q000235:genetics*; D005314:Embryonic and Fetal Development; D005260:Female; D005455:Fluorescent Antibody Technique; D006579:Heterozygote; D051379:Mice; D008815:Mice, Inbred Strains; D008854:Microscopy, Electron; D009154:Mutation; D010641:Phenotype; D011247:Pregnancy