Insulin-like material from the digestive tract of the tunicate Pyura pachydermatina (sea tulip).
Gut tissue from the tunicate Pyura pachydermatina (sea tulip) was found to contain a compound or compounds which react with anti-porcine insulin antibodies, but not anti-hag-fish insulin antibodies, and which also stimulate lipogenesis in isolated rat fat cells. The insulin-like material is present in two immunologically active forms, a species of Mr 6000 apparently similar to mammalian insulin, and a high Mr form which expresses biological activity only after further purification by reverse-phase HPLC. The bioactivity of both species is suppressed in the presence of anti-porcine insulin antibodies.
Galloway
S M
SM
Department of Biochemistry, University of Otago, Dunedin, New Zealand.
Cutfield
J F
JF
D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004064:Digestive System / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D066298:In Vitro Techniques; D007328:Insulin / Q000302:isolation & purification* / Q000502:physiology; D008055:Lipids / Q000096:biosynthesis; D008970:Molecular Weight; D011863:Radioimmunoassay; D051381:Rats; D014561:Urochordata / Q000032:analysis*
D007328:Insulin; D008055:Lipids