Promoter vectors with restriction-site banks.
New vectors harboring the promoter for the chloramphenicol acetyl transferase gene (cat promoter) have been constructed. These vectors are all derived from pJRD184 [Heusterspreute et al., Gene 39 (1985) 299-304], which contains a restriction-site bank. The cat promoter has been inserted at various positions and in reverse orientations so that almost all the restriction sites originally present on JRD184 can be used in cloning experiments. The expression of the aceK gene of Escherichia coli cloned under the control of the cat promoter has been tested. A large increase in the synthesis of the isocitrate dehydrogenase kinase, the aceK gene product, has demonstrated the efficiency of the newly constructed vectors.
Bleicher
F
F
Laboratoire de Biologie Moléculaire, Université de Lyon, Villeurbanne, France.
Deville
F
F
Cortay
J C
JC
Nègre
D
D
Cozzone
A J
AJ
Cenatiempo
Y
Y
D000123:Acetyltransferases / Q000235:genetics; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics; D015500:Chloramphenicol O-Acetyltransferase; D003001:Cloning, Molecular / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D005822:Genetic Vectors; D011401:Promoter Regions, Genetic; D011494:Protein Kinases / Q000096:biosynthesis / Q000235:genetics; D017346:Protein Serine-Threonine Kinases; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics
D001426:Bacterial Proteins; D011993:Recombinant Fusion Proteins; D000123:Acetyltransferases; D015500:Chloramphenicol O-Acetyltransferase; D011494:Protein Kinases; D017346:Protein Serine-Threonine Kinases; C042301:isocitrate dehydrogenase kinase