Bombesin and its analogues inhibit interleukin-2-induced proliferation of CTLL-2 cells.
Specifically interleukin-2 (IL-2)-dependent CTLL-2 cells were incubated in short term culture in the presence of IL-2 together with bombesin and two analogues, [Lys3]bombesin and [Tyr4]bombesin in different concentrations. Cell proliferation, determined by [3H]thymidine incorporation was significantly inhibited by 35.6 +/- 5%, 39.0 +/- 5.6% and 57.0 +/- 11% (mean +/- S.E.M. of 3 independent experiments). A typically U-shaped dose-response relationship was observed, showing a maximum effect between 10(-12) and 10(-10) M. Our data support the hypothesis that this effect is mediated by a specific receptor for bombesin and closely related peptides on CTLL-2 cells. As IL-2 plays a critical role in the clonal expansion of activated lymphocytes, antagonism of the effect of IL-2 is of high biological significance.
Fink
R
R
II. Medizinische Klinik und Poliklinik, Technischen Universität München, F.R.G.
Ehrhardt
R
R
Dancygier
H
H
D000818:Animals; D001839:Bombesin / Q000031:analogs & derivatives / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects*; D002460:Cell Line; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D006728:Hormones / Q000494:pharmacology; D007376:Interleukin-2 / Q000494:pharmacology*; D051379:Mice; D051381:Rats; D011917:Rats, Inbred Lew; D013154:Spleen / Q000166:cytology
D006728:Hormones; D007376:Interleukin-2; D001839:Bombesin