The acrosome reaction in boar spermatozoa.
In order to gain more insight into the molecular alterations of the acrosome, boar spermatozoa were incubated in a calcium-containing medium in the presence of the ionophore A23187. The time-course of the acrosome reaction was assessed by phase-contrast microscopy. Different stages of the acrosome reaction were studied by immunoelectron microscopy using a specific antibody directed against the complete outer acrosomal membrane. The introduction of monoclonal antibodies generated by immunization of Balb/c mice with the isolated outer acrosomal membrane permitted a study of the topography of distinct membrane proteins during the acrosome reaction. The exposure of activation of a fucose-binding protein important for sperm--zona attachment was studied using neo-glycoproteins labelled with colloidal gold for transmission electron microscopy.
Töpfer-Petersen
E
E
Department of Dermatology, University of Munich, FRG.
Friess
A E
AE
Schill
W B
WB
D000177:Acrosome / Q000276:immunology / Q000648:ultrastructure; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D005643:Fucose / Q000276:immunology*; D066298:In Vitro Techniques; D037102:Lectins / Q000276:immunology*; D008297:Male; D008854:Microscopy, Electron; D013094:Spermatozoa / Q000276:immunology / Q000502:physiology* / Q000648:ultrastructure; D013552:Swine
D000911:Antibodies, Monoclonal; D037102:Lectins; C027112:fucose-binding lectin; D005643:Fucose