Characteristics and applications of adsorbents for pyrogen removal.
Characteristics and applications of immobilized histidine and immobilized histamine for pyrogen removal were investigated. Immobilized histidine showed a high affinity for pyrogen at low ionic strength and over a wide pH range. The adsorption capacity was 0.53 mg of lipopolysaccharide per milliliter of the adsorbent. The apparent dissociation constant was 1.57 X 10(-9) M. The adsorption of pyrogen to immobilized histidine decreased with increasing ionic strength, but pyrogen could be adsorbed even at ionic strengths of gamma/2 = 0.05-0.1, at which other substances were little adsorbed; that is, specific adsorption of pyrogen was observed. The adsorption of pyrogen could be increased at ionic strengths of gamma/2 = 0.05-0.1 by using a lower flow rate or a longer column length. Immobilized histidine and immobilized histamine could be used for the removal of natural pyrogens contaminating various useful low-molecular-weight compounds as well as high-molecular-weight compounds such as proteins.
Minobe
S
S
Department of Biochemistry, Tanabe Seiyaku Co.Ltd., Osaka, Japan.
Watanabe
T
T
Sato
T
T
Tosa
T
T
D000327:Adsorption; D002846:Chromatography, Affinity / Q000379:methods; D004926:Escherichia coli; D006632:Histamine; D006639:Histidine; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D007700:Kinetics; D011749:Pyrogens / Q000302:isolation & purification*
D007202:Indicators and Reagents; D011749:Pyrogens; D006639:Histidine; D006632:Histamine