Rapid antibiotic susceptibility tests on Enterobacteriaceae by ATP bioluminescence.
The susceptibility of 76 clinical isolates of Enterobacteriaceae to ampicillin, piperacillin and gentamicin was assessed by ATP bioluminescence in a 4-h test. For most organisms tested (Escherichia, Klebsiella, Enterobacter and Serratia), there was good correlation with traditional MIC values estimated on 18-h cultures. However strains of Proteus mirabilis showed false resistance to the beta-lactam agents with the ATP method; and concordance was achieved only after manipulation of the growth conditions. Our method is simpler than those described previously, though currently it is still labour-intensive and expensive.
Wheat
P F
PF
Department of Bacteriology, Royal Hallamshire Hospital, Sheffield.
Hastings
J G
JG
Spencer
R C
RC
D000255:Adenosine Triphosphate / Q000032:analysis*; D000667:Ampicillin / Q000494:pharmacology; D004755:Enterobacteriaceae / Q000187:drug effects* / Q000378:metabolism; D005839:Gentamicins / Q000494:pharmacology; D008163:Luminescent Measurements; D008826:Microbial Sensitivity Tests / Q000379:methods*; D009994:Osmolar Concentration; D010878:Piperacillin / Q000494:pharmacology; D011513:Proteus mirabilis / Q000187:drug effects / Q000378:metabolism
D005839:Gentamicins; D000667:Ampicillin; D000255:Adenosine Triphosphate; D010878:Piperacillin