[Immune electron microscope localization of RNA-polymerase on the Escherichia coli chromosome using monoclonal antibodies against the beta-subunit].
Possibility of the immunoelectron microscopic visualization of RNA polymerase on the Escherichia coli chromosome with monoclonal antibodies against the beta-subunit labelled by [protein A.gold] complex was demonstrated. Using this method RNA polymerase molecules were revealed within nucleoid as well as on the membrane-free chromosome.
Grachev
M A
MA
Zaĭchikov
E F
EF
Laktionov
P P
PP
Roshke
V V
VV
D000911:Antibodies, Monoclonal; D002876:Chromosomes, Bacterial; D012321:DNA-Directed RNA Polymerases / Q000032:analysis*; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics / Q000648:ultrastructure; D008854:Microscopy, Electron
D000911:Antibodies, Monoclonal; D012321:DNA-Directed RNA Polymerases
Immuno-élektronno-mikroskopicheskaia lokalizatsiia RNK-polimerazy na khromosome Escherichia coli pri pomoshchi monoklonal'nykh antitel protiv beta-sub''edinitsy.