Quantification of renin mRNA in various mouse tissues by a novel solution hybridization assay.
A new solution hybridization assay was established for the measurement of renin mRNA. The assay makes use of a radioactively labelled renin complementary RNA as hybridization probe and allows rapid and sensitive detection of renin mRNA in amounts as low as 0.5 pg. With this assay it was possible to quantify renin mRNA in tissues with low-gene expression such as brain and heart as well as in testis, kidney and the submandibular gland (SMG) of mice. The concentrations in these organs were 0.03, 0.07, 1, 5.2 and 164 pg renin mRNA/micrograms RNA, respectively. These results are in agreement with Northern blotting experiments. The general applicability and ease of the solution hybridization assay described here should greatly improve the rapidity of mRNA measurements in future functional studies.
Paul
M
M
German Institute for High Blood Pressure Research, Heidelberg, FRG.
Wagner
D
D
Metzger
R
R
Ganten
D
D
Lang
R E
RE
Suzuki
F
F
Murakami
K
K
Burbach
J H
JH
Ludwig
G
G
D000818:Animals; D008297:Male; D051379:Mice; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000032:analysis* / Q000235:genetics; D012083:Renin / Q000032:analysis* / Q000235:genetics; D014018:Tissue Distribution
D012333:RNA, Messenger; D012083:Renin