title: [Application to an automatic system of a direct method for determining atypical variants of cholinesterase using succinyldithiocholine as substrate].
abstract: The determination of human serum cholinesterase (EC 3.1.1.8) is frequently requested to detect patients with atypical forms of the enzyme which reacts abnormally with succinylcholine (suxamethonium), employed as a neuromuscular blocking agent. Usually, for biochemical identification of succinylcholine sensitive individuals the standard reaction is run with and without the inhibitors, notably dibucaine. A new test for direct determination of succinylcholine sensitive individuals which use a substrate analogue of succinylcholine was applied to a automated instrument. The linearity, precision, recovery, interference and correlation of the method have been evaluated. We have estimated the reference intervals for a population of 364 healthy subjects subdivided for sex, three atypical homozygotes and two atypical heterozygotes. On the basis of analytical performance we can conclude that this test may offer a further parameter for preoperative screening of individuals with an abnormal response to the muscle relaxant succinylcholine, thus avoiding the determination of genotype by measurement of dibucaine number.
authors.affiliation: Laboratorio di analisi chimico-cliniche e microbiologiche, Ospedale - U.S.L. 25, Bentivoglio, Bologna.
authors.forename: G
authors.initials: G
authors.lastname: Capone
authors.forename: D
authors.initials: D
authors.lastname: Gnudi
authors.forename: L
authors.initials: L
authors.lastname: Pensabeni
authors.forename: A M
authors.initials: AM
authors.lastname: Martelli
authors.forename: M C
authors.initials: MC
authors.lastname: Marconi
authors.forename: A
authors.initials: A
authors.lastname: Accorsi
mesh_terms: D001663:Bilirubin / Q000097:blood; D002802:Cholinesterases / Q000097:blood* / Q000145:classification; D005069:Evaluation Studies as Topic; D005260:Female; D006454:Hemoglobins / Q000032:analysis; D006801:Humans; D008297:Male; D011933:Reagent Kits, Diagnostic; D013390:Succinylcholine
chemical_list: D006454:Hemoglobins; D011933:Reagent Kits, Diagnostic; D002802:Cholinesterases; D013390:Succinylcholine; D001663:Bilirubin
vernacular_title: Applicazione a sistema automatico di un metodo diretto per la determinazione delle varianti atipiche della colinesterasi con succinilditiocolina come substrato.