Local administration of cells containing an inserted IL-2 gene and producing IL-2 inhibits growth of human tumours in nu/nu mice.
We have prepared a retroviral expression construct, pPS-IL-2, in which human IL-2 cDNA has been inserted into the polylinker region, and have used the retroviral vector to introduce the functional IL-2 gene into a fibroblast cell line, RAT-1. Peritumoral administration of IL-2-producing RAT-1 cells into congenitally athymic (nu/nu) mice carrying subcutaneous transplants of human carcinoma cells inhibited the growth of the human tumour xenografts.
Bubenik
J
J
Institute of Molecular Genetics, Czechoslovak Academy of Sciences, Prague.
Voitenok
N N
NN
Kieler
J
J
Prassolov
V S
VS
Chumakov
P M
PM
Bubenikova
D
D
Simova
J
J
Jandlova
T
T
D000818:Animals; D005347:Fibroblasts / Q000276:immunology / Q000637:transplantation; D005818:Genetic Engineering; D005822:Genetic Vectors; D006367:HeLa Cells / Q000276:immunology / Q000473:pathology; D006801:Humans; D007376:Interleukin-2 / Q000096:biosynthesis / Q000235:genetics*; D007694:Killer Cells, Natural / Q000276:immunology; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000235:genetics / Q000276:immunology / Q000628:therapy*; D012190:Retroviridae / Q000235:genetics; D014170:Transformation, Genetic
D007376:Interleukin-2