Excision of apurinic and/or apyrimidinic sites from DNA by nucleolytical enzymes from rat brain.
Apurinic and/or apyrimidinic (AP) sites were excised from PM2 phage DNA by two enzymes: an AP endodeoxyribonuclease isolated from rat neocortex chromatin and a rat brain exodeoxyribonuclease, DNase B III. The resulting gap was filled with DNA polymerase beta prepared from rat liver and finally ligated by Escherichia coli DNA ligase.
Ivanov
V A
VA
Institute of Biological Physics, Academy of Sciences of the USSR, Pushchino.
Tretyak
T M
TM
Afonin
Y N
YN
D000818:Animals; D001080:Apurinic Acid / Q000378:metabolism*; D001665:Binding Sites; D001921:Brain / Q000201:enzymology / Q000378:metabolism*; D002540:Cerebral Cortex / Q000378:metabolism; D004247:DNA / Q000096:biosynthesis; D011088:DNA Ligases / Q000378:metabolism; D004256:DNA Polymerase I / Q000378:metabolism; D004260:DNA Repair; D004706:Endodeoxyribonucleases / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology; D005090:Exodeoxyribonucleases / Q000378:metabolism; D066298:In Vitro Techniques; D011119:Polynucleotides / Q000378:metabolism*; D051381:Rats
D011119:Polynucleotides; C001790:apyrimidinic acid; D001080:Apurinic Acid; D004247:DNA; D004256:DNA Polymerase I; D004706:Endodeoxyribonucleases; D005090:Exodeoxyribonucleases; D011088:DNA Ligases