title: [Immune electron microscope localization of RNA-polymerase on the Escherichia coli chromosome using monoclonal antibodies against the beta-subunit].
abstract: Possibility of the immunoelectron microscopic visualization of RNA polymerase on the Escherichia coli chromosome with monoclonal antibodies against the beta-subunit labelled by [protein A.gold] complex was demonstrated. Using this method RNA polymerase molecules were revealed within nucleoid as well as on the membrane-free chromosome.
authors.forename: M A
authors.initials: MA
authors.lastname: Grachev
authors.forename: E F
authors.initials: EF
authors.lastname: Zaĭchikov
authors.forename: P P
authors.initials: PP
authors.lastname: Laktionov
authors.forename: V V
authors.initials: VV
authors.lastname: Roshke
mesh_terms: D000911:Antibodies, Monoclonal; D002876:Chromosomes, Bacterial; D012321:DNA-Directed RNA Polymerases / Q000032:analysis*; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics / Q000648:ultrastructure; D008854:Microscopy, Electron
chemical_list: D000911:Antibodies, Monoclonal; D012321:DNA-Directed RNA Polymerases
vernacular_title: Immuno-élektronno-mikroskopicheskaia lokalizatsiia RNK-polimerazy na khromosome Escherichia coli pri pomoshchi monoklonal'nykh antitel protiv beta-sub''edinitsy.