title: Evidence for the association of dystrophin with the transverse tubular system in skeletal muscle.
abstract: Polyclonal antibodies to dystrophin (the protein product of the human Duchenne muscular dystrophy gene) were used to identify and characterize dystrophin in isolated triads from rabbit skeletal muscle. Anti-dystrophin antibodies recognize an approximately 400,000-Da protein in isolated triads or heavy microsomes from skeletal muscle. Treatment of heavy microsomes with buffers containing high salt or EDTA to remove peripheral or extrinsic membrane proteins does not remove dystrophin; however, treatment of intact triads with trypsin shows that dystrophin is extremely sensitive to mild proteolytic digestion. Isolation of junctional complexes from skeletal muscle triads indicates that dystrophin is tightly associated with the triadic junction. Fractionation of the triadic junction into junctional transverse tubular membranes and junctional sarcoplasmic reticulum membranes has shown that dystrophin is enriched in junctional transverse tubular membranes. Thus, our results suggest that dystrophin is a component of the triad junction which is exposed to the cytoplasm and embedded in or attached to the transverse tubular membrane.
authors.affiliation: Department of Physiology and Biophysics, University of Iowa College of Medicine, Iowa City 52242.
authors.forename: C M
authors.initials: CM
authors.lastname: Knudson
authors.forename: E P
authors.initials: EP
authors.lastname: Hoffman
authors.forename: S D
authors.initials: SD
authors.lastname: Kahl
authors.forename: L M
authors.initials: LM
authors.lastname: Kunkel
authors.forename: K P
authors.initials: KP
authors.lastname: Campbell
mesh_terms: D000818:Animals; D000906:Antibodies; D016189:Dystrophin; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007163:Immunosorbent Techniques; D008854:Microscopy, Electron; D008970:Molecular Weight; D009124:Muscle Proteins / Q000032:analysis*; D009132:Muscles / Q000032:analysis*; D011817:Rabbits
chemical_list: D000906:Antibodies; D016189:Dystrophin; D009124:Muscle Proteins