Characterization and localization of the products of the human homologs of the v-ets oncogene.
The avian erythroblastosis virus, E26, an acute leukemia virus, contains a transforming gene composed of two cellular components, v-myb and v-ets. The v-ets related sequences of man and other mammals consist of two transcriptionally active genes, ets-1 and ets-2, located on separate chromosomes. By contrast, both of these genes are contiguous in birds, are located on the same chromosome, and are coordinately transcribed. The human ets-1 and ets-2 gene products were identified by means of antibodies directed against the ets-1 and ets-2 encoded products. A 51 kD protein has been identified as the ets-1 gene product, and a 56 kD protein as the ets-2 gene product. Cellular fractionation studies indicated that the ets-1 protein is located in the cytoplasm and the ets-2 protein is nuclear. By comparison, the chicken ets protein, which contains both the ets-1 and ets-2 domains, distributes equally between the cytoplasm and nucleus. The differential compartmentalization of the ets gene products and their non-coordinate expression suggest that these proteins have different biological functions.
Fujiwara
S
S
Laboratory of Molecular Oncology, National Cancer Institute, Frederick, Maryland.
Fisher
R J
RJ
Seth
A
A
Bhat
N K
NK
Showalter
S D
SD
Zweig
M
M
Papas
T S
TS
D002451:Cell Compartmentation; D006801:Humans; D007158:Immunologic Techniques; D008970:Molecular Weight; D010449:Peptide Mapping; D011518:Proto-Oncogene Proteins / Q000502:physiology*; D011519:Proto-Oncogenes; D013347:Subcellular Fractions / Q000032:analysis
D011518:Proto-Oncogene Proteins