Immunological identification of a high molecular weight protein as a candidate for the product of the Duchenne muscular dystrophy gene.
An oligopeptide was synthesized based on translation of the nucleotide sequence of the putative exon region of clone pERT87-25 from the gene for Duchenne muscular dystrophy. Immunization of rabbits with this oligopeptide induced the formation of antibodies directed against a protein present in human, rat, and rabbit skeletal muscle. This protein, which is missing in the skeletal muscle of two patients with Duchenne muscular dystrophy, has a molecular mass of approximately equal to 320-420 kDa and is clearly different from the putative Duchenne muscular dystrophy-related protein nebulin. The data suggest that this 320- to 420-kDa protein is produced by the Duchenne muscular dystrophy gene.
Kao
L
L
Department of Biochemistry and Molecular Biology, University of Cincinnati College of Medicine, OH 45267-0522.
Krstenansky
J
J
Mendell
J
J
Rammohan
K W
KW
Gruenstein
E
E
D000936:Antigen-Antibody Complex; D003429:Cross Reactions; D016189:Dystrophin; D004591:Electrophoresis, Polyacrylamide Gel; D005091:Exons; D005796:Genes; D006801:Humans; D008970:Molecular Weight; D009124:Muscle Proteins / Q000032:analysis / Q000235:genetics*; D009132:Muscles / Q000378:metabolism*; D009136:Muscular Dystrophies / Q000235:genetics*
D000936:Antigen-Antibody Complex; D016189:Dystrophin; D009124:Muscle Proteins