Enzyme immunoassay measurement of thymosin beta 4 in human serum.
An enzyme immunoassay (EIA) for measurement of human serum thymosin beta 4 is described. Antiserum to synthetic thymosin beta 4, raised in rabbits, is incubated with a standard or serum. The unbound antibody in liquid-phase then binds with solid-phase thymosin beta 4. The EIA is both sensitive and accurate and is capable of detecting as little as 2.5 ng/ml thymosin beta 4 in serum. No cross-reactivity was observed with common serum proteins or other putative thymic hormones. High-performance liquid chromatography of serum samples reveals a single thymosin beta 4 peak that corresponds to the authentic thymosin beta 4 peak in the standard. Human serum levels range from 3 to 82 ng/ml in 142 healthy adult human volunteers. Newborn cord serum levels of thymosin beta 4 are lower than in adults.
Weller
F E
FE
Department of Medicine, Wayne State University School of Medicine, Detroit, Michigan.
Mutchnick
M G
MG
Goldstein
A L
AL
Naylor
P H
PH
D000469:Alkaline Phosphatase / Q000378:metabolism; D000918:Antibody Specificity; D002851:Chromatography, High Pressure Liquid; D005312:Fetal Blood / Q000032:analysis / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques; D066298:In Vitro Techniques; D007231:Infant, Newborn; D012015:Reference Standards; D012044:Regression Analysis; D000077596:Thymalfasin; D013947:Thymosin / Q000031:analogs & derivatives* / Q000032:analysis / Q000097:blood / Q000276:immunology; D000099201:Thymosin beta(4)
D000469:Alkaline Phosphatase; D000077596:Thymalfasin; D013947:Thymosin; D000099201:Thymosin beta(4)