title: Specific antibody against a protein (p27) present in nonestablished fibroblasts. A putative microfilament-associated protein.
abstract: A specific polyclonal antibody has been raised against a basic cytoplasmic protein (p27) which is induced by serum in growth-arrested NIH 3T3 cells but is constitutively expressed in nonestablished fibroblasts. Immunoblotting analysis and [35S]methionine labeling show that p27 is absent in tissues and established cell lines of different types. However, it is present in fibroblasts from human, rat, mouse, and chicken origin and is highly conserved as determined by two-dimensional gel electrophoresis. Double immunofluorescence shows that p27 colocalizes with actin filaments. These observations would suggest that p27 is an actin-associated protein expressed in nonestablished fibroblasts.
authors.affiliation: European Molecular Biology Laboratory, Heidelberg, West Germany.
authors.forename: J F
authors.initials: JF
authors.lastname: Santarén
authors.forename: H
authors.initials: H
authors.lastname: Blüthmann
authors.forename: H
authors.initials: H
authors.lastname: MacDonald-Bravo
authors.forename: R
authors.initials: R
authors.lastname: Bravo
mesh_terms: D000375:Aging; D000818:Animals; D000918:Antibody Specificity; D002460:Cell Line; D002642:Chick Embryo; D003593:Cytoplasm / Q000032:analysis / Q000378:metabolism; D005314:Embryonic and Fetal Development; D005347:Fibroblasts / Q000032:analysis* / Q000378:metabolism / Q000502:physiology; D005455:Fluorescent Antibody Technique; D006801:Humans; D051379:Mice; D008840:Microfilament Proteins / Q000032:analysis* / Q000096:biosynthesis / Q000276:immunology; D011817:Rabbits; D051381:Rats; D014018:Tissue Distribution
chemical_list: D008840:Microfilament Proteins