Inhibition of cell surface ruffling and fluid-phase pinocytosis by microinjection of anti-ras antibodies into living cells.
Fibroblasts transformed by ras oncogenes display enhanced cell surface ruffling and fluid-phase pinocytotic activities. Microinjection of antibodies that specifically bind the ras proteins into these cells results in the inhibition of these two surface activities. The possible underlying biochemical basis of the influence of the ras proteins on membrane ruffling and pinocytosis and the potential relationship of these two biological activities to membrane signal transduction are discussed.
Bar-Sagi
D
D
Cold Spring Harbor Laboratory, New York 11724.
McCormick
F
F
Milley
R J
RJ
Feramisco
J R
JR
D000818:Animals; D000906:Antibodies / Q000008:administration & dosage*; D002462:Cell Membrane / Q000276:immunology / Q000648:ultrastructure*; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D011905:Genes, ras; D006801:Humans; D008560:Membrane Fluidity; D010873:Pinocytosis; D011518:Proto-Oncogene Proteins / Q000276:immunology*; D016283:Proto-Oncogene Proteins p21(ras); D051381:Rats; D013499:Surface Properties
D000906:Antibodies; D011518:Proto-Oncogene Proteins; C501009:HRAS protein, human; D016283:Proto-Oncogene Proteins p21(ras)