Expression of cDNA encoding human basic fibroblast growth factor in E. coli.
The cDNA encoding human basic fibroblast growth factor was expressed in E. coli under the control of trp promoter. Bacterially synthesized hbFGF was highly purified using a heparin affinity HPLC column. By this chromatography, hbFGF was eluted as four distinct forms, which were indistinguishable by SDS polyacrylamide gel electrophoresis, amino acid composition, and partial terminal sequence analysis. These molecules stimulated the growth of fibroblasts and endothelial cells although their specific activities varied. The angiogenesis activity of these molecules was also confirmed.
Iwane
M
M
Kurokawa
T
T
Sasada
R
R
Seno
M
M
Nakagawa
S
S
Igarashi
K
K
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000032:analysis*; D004926:Escherichia coli / Q000235:genetics*; D005346:Fibroblast Growth Factors / Q000235:genetics*; D005786:Gene Expression Regulation; D006801:Humans; D010957:Plasmids; D011994:Recombinant Proteins / Q000032:analysis / Q000235:genetics
D000596:Amino Acids; D011994:Recombinant Proteins; D005346:Fibroblast Growth Factors; D004247:DNA