title: EKTACHEM bilirubin fraction Bc as a predictor of liver transplant rejection.
abstract: Bilirubin fractions Bc and DELTA, not routinely available prior to the EKTACHEM Chemistry Analyzer and its slide methodology, were studied in an outpatient population of liver transplant recipients. A preliminary evaluation by the authors has shown that direct bilirubin (DBILI) levels in the normal range consist almost exclusively of DELTA (protein-bound conjugated bilirubin), while at elevated DBILI levels, an increasing amount of Bc (non-protein-bound conjugated bilirubin) is measured as well. The present study evaluated the clinical significance of Bc in the serum of 80 liver transplant recipients as a means of identifying episodes of rejection. Each patient was classified into rejection or nonrejection categories based on clinical status, liver biopsy results, and/or response to therapy. Eighteen patients were classified as experiencing an episode of rejection during the period of this study. Fourteen of these (77.8%) had Bc levels that ranged from 0.1 to 6.8 mg/dl. Sixty two patients were classified in the nonrejection category. Fourteen (22.6%) of these patients had Bc levels that ranged from 0.1 to 0.6 mg/dl. In our outpatient liver transplant recipients with Bc greater than or equal to 0.1 mg/dl, the relative risk of rejection (% of rejection patients with Bc/% of nonrejection patients with Bc) was 3.44. This value indicates that Bc determination may be a helpful adjunct in the assessment of rejection.
authors.affiliation: Division of Ambulatory Laboratory Medicine, University Health Center of Pittsburgh, Pennsylvania.
authors.forename: C J
authors.initials: CJ
authors.lastname: Cox
authors.forename: R O
authors.initials: RO
authors.lastname: Valdiserri
authors.forename: T R
authors.initials: TR
authors.lastname: Zerbe
authors.forename: J L
authors.initials: JL
authors.lastname: Genter
mesh_terms: D001663:Bilirubin / Q000097:blood*; D001774:Blood Chemical Analysis / Q000295:instrumentation*; D006084:Graft Rejection; D006801:Humans; D016031:Liver Transplantation; D011237:Predictive Value of Tests; D011379:Prognosis
chemical_list: D001663:Bilirubin