Sandwich enzyme immunoassay for human interleukin-1 beta (hIL-1 beta) in urine.
A highly sensitive sandwich enzyme immunoassay for human interleukin-1 beta (hIL-1 beta) in urine is described. Rabbit anti-hIL-1 beta IgG-coated polystyrene balls were incubated with urine samples and subsequently with affinity-purified rabbit anti-hIL-1 beta Fab'-horseradish peroxidase conjugate. After washing, the peroxidase activity bound to the polystyrene ball was assayed by fluorimetry using 3-(4-hydroxyphenyl)propionic acid as a substrate. The detection limit of hIL-1 beta was 0.5 pg/tube (30 amol/tube) or 5 ng/1 of urine when 0.1 ml of urine sample was used. The molecular weight of hIL-1 beta in normal urine was shown to be 17,000 by gel filtration, and levels of urine hIL-1 beta in healthy subjects aged 19-87 yr were 0-146 ng/1 or 0-88 ng/g of creatinine.
Tanaka
K
K
Ishikawa
E
E
Ohmoto
Y
Y
Hirai
Y
Y
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D002850:Chromatography, Gel; D003956:Dialysis; D005260:Female; D006735:Horseradish Peroxidase; D006801:Humans; D007124:Immunoenzyme Techniques; D007375:Interleukin-1 / Q000652:urine*; D008297:Male; D008875:Middle Aged; D011817:Rabbits; D013050:Spectrometry, Fluorescence
D007375:Interleukin-1; D006735:Horseradish Peroxidase