Two integral membrane proteins located in the cis-middle and trans-part of the Golgi system acquire sialylated N-linked carbohydrates and display different turnovers and sensitivity to cAMP-dependent phosphorylation.
The localization and chemical characteristics of two Golgi integral membrane proteins (GIMPs) have been studied using monoclonal antibodies. The two proteins are segregated in different parts of the Golgi system and whereas GIMPc(130 kD) is located in the cis and medial cisternae, GIMPt (100 kD) is confined in the trans-most cisterna and trans-tubular network. Both GIMPs are glycoproteins that contain N- and O-linked carbohydrates. The N-linked carbohydrates were exclusively of the complex type. Although excluded from the trans-side of the Golgi system, where sialylation is believed to occur, GIMPc acquires sialic acid in both its N- and O-linked carbohydrates. Sialic acid was also detected in the N-linked carbohydrates of GIMPt. GIMPc is apparently phosphorylated in the luminal domain in vivo. Phosphorylation occurred exclusively on serine and was stimulated by dibutyryl cyclic AMP. GIMPc and GIMPt displayed half-lives of 20 and 9 h, respectively.
Yuan
L
L
Barriocanal
J G
JG
Bonifacino
J S
JS
Sandoval
I V
IV
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001491:Basophils; D050260:Carbohydrate Metabolism; D002460:Cell Line; D005455:Fluorescent Antibody Technique; D006023:Glycoproteins / Q000276:immunology / Q000378:metabolism*; D006056:Golgi Apparatus / Q000378:metabolism*; D007668:Kidney; D007942:Leukemia, Experimental / Q000473:pathology; D008562:Membrane Glycoproteins; D008565:Membrane Proteins / Q000276:immunology / Q000378:metabolism*; D019158:N-Acetylneuraminic Acid; D010766:Phosphorylation; D010768:Phosphoserine / Q000378:metabolism; D011494:Protein Kinases / Q000378:metabolism; D011498:Protein Precursors / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D051381:Rats; D012794:Sialic Acids / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D006023:Glycoproteins; C053330:Golgi membrane glycoproteins; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D011498:Protein Precursors; D012794:Sialic Acids; D010768:Phosphoserine; D011494:Protein Kinases; D019158:N-Acetylneuraminic Acid