title: A yeast operator overlaps an upstream activation site.
abstract: The product of the BAR1 gene of Saccharomyces cerevisiae is synthesized only in the a cell type and inactivates alpha-factor, the mating pheromone made by alpha cells. The MAT alpha 2 protein represses the transcription of a-cell-specific genes, including BAR1, in alpha and a/alpha diploid cells. Transcription of BAR1 in a cells in stimulated upon exposure to alpha-factor. Deletion analysis of the 5' noncoding region of the BAR1 gene revealed that the major upstream activation site (UAS) overlaps the 31 bp operator sequence required for MAT alpha 2 repression. This result has implications for the negative control of transcription in yeast. The deletion analysis also indicated that the sequence TGAAACA mediates alpha-factor stimulation.
authors.forename: J W
authors.initials: JW
authors.lastname: Kronstad
authors.forename: J A
authors.initials: JA
authors.lastname: Holly
authors.forename: V L
authors.initials: VL
authors.lastname: MacKay
mesh_terms: D001483:Base Sequence; D005786:Gene Expression Regulation; D005800:Genes, Fungal; D009154:Mutation; D009875:Operator Regions, Genetic; D010675:Pheromones / Q000235:genetics; D012097:Repressor Proteins / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D010675:Pheromones; D012097:Repressor Proteins