title: Precise specificity of induced tubular HLA-class II antigens in renal allografts.
abstract: HLA-class II antigen expression is induced in the tubules of renal allografts, but it is unclear whether all three class II products--HLA-DR, DQ, and DP--are induced, and whether the induced product is of donor origin. A pretransplant (n = 14) and serial transplant biopsies (n = 45) were obtained from 14 transplant recipients in whom induced HLA-class II antigen was detected after transplantation with a monoclonal antibody reactive with HLA-DR, DP, and possibly DQ antigens. Cryostat sections were stained with locus-specific or polymorphic monoclonal antibodies in an indirect immunoperoxidase assay. In pretransplant biopsies intracellular HLA-DR antigen was expressed on proximal tubules, whereas all tubules were negative for HLA-DQ and DP products. After transplantation grafts with induced tubular HLA-class II antigen had induced HLA-DR, DQ and DP antigens expressed both within the cytoplasm and on the cell membranes. The donor or recipient origin of induced HLA-class II expression was determined using polymorphic antibodies specific for either donor or recipient antigens. This approach demonstrated that the induced class II antigen is of donor origin--and, furthermore, that the renal parenchyma remains of donor HLA-type, even one year after transplantation, and thus remains a source of antigenic stimulus to the recipient.
authors.forename: S V
authors.initials: SV
authors.lastname: Fuggle
authors.forename: D L
authors.initials: DL
authors.lastname: McWhinnie
authors.forename: P J
authors.initials: PJ
authors.lastname: Morris
mesh_terms: D000911:Antibodies, Monoclonal; D001706:Biopsy; D006681:HLA-D Antigens / Q000276:immunology*; D006801:Humans; D007124:Immunoenzyme Techniques; D016030:Kidney Transplantation; D007684:Kidney Tubules / Q000276:immunology*; D011110:Polymorphism, Genetic
chemical_list: D000911:Antibodies, Monoclonal; D006681:HLA-D Antigens