Epidermal growth factor binding protein: identification of a different protein.
Partial amino acid sequence analysis of epidermal growth factor binding protein (EGF-BP), an arginine esteropeptidase that specifically associates with EGF to form a high molecular weight complex in male mouse submandibular glands, has revealed a single, distinct protein that is different from three previously reported forms of EGF-BP. This protein shows substantial sequence homology with these other putative forms of EGF-BP as well as with a large family of kallikreins expressed in the mouse submandibular gland. Purified EGF-BP contains three polypeptide chains as a result of two internal cleavages at residues 87-88 and 140-141. These modifications may represent processing events that are critical for determining the binding specificity of EGF-BP, since they occur within regions surrounding the substrate binding site.
Isackson
P J
PJ
Silverman
R E
RE
Blaber
M
M
Server
A C
AC
Nichols
R A
RA
Shooter
E M
EM
Bradshaw
R A
RA
D000595:Amino Acid Sequence; D000818:Animals; D010450:Endopeptidases / Q000302:isolation & purification / Q000378:metabolism*; D004815:Epidermal Growth Factor / Q000378:metabolism*; D007610:Kallikreins; D008297:Male; D051379:Mice; D013329:Structure-Activity Relationship; D013363:Submandibular Gland / Q000378:metabolism*; D020840:Tissue Kallikreins; D014961:X-Ray Diffraction
D004815:Epidermal Growth Factor; D010450:Endopeptidases; D007610:Kallikreins; C489120:Klk13 protein, mouse; D020840:Tissue Kallikreins; C489121:Klk1b22 protein, mouse; C489119:Klk9 protein, mouse; C053223:epidermal growth factor binding protein, mouse