Immunohistochemical studies on GABAergic neurons in the rat locus coeruleus, with special reference to their relationship to astrocytes.
Purified antisera against GABA were prepared. A few small GABAergic neurons in the rat locus coeruleus were immunohistochemically demonstrated by both the unlabeled peroxidase-antiperoxidase method and the avidin-biotin peroxidase complex method using affinity-purified GABA antibodies. The glial fibrillary acidic protein immunoreactivity in this nucleus was localized by the latter method in the astrocytal framework encircling medium-sized and small neurons as well as in straight processes. Astrocytes may play a role as energy donors to these neurons.
Iijima
K
K
Kobayashi
R
R
Kojima
N
N
D000818:Animals; D000906:Antibodies / Q000302:isolation & purification; D001253:Astrocytes / Q000032:analysis*; D005260:Female; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D006651:Histocytochemistry; D007124:Immunoenzyme Techniques; D008125:Locus Coeruleus / Q000032:analysis* / Q000166:cytology; D009474:Neurons / Q000032:analysis* / Q000145:classification; D051381:Rats; D011919:Rats, Inbred Strains; D005680:gamma-Aminobutyric Acid / Q000032:analysis* / Q000276:immunology
D000906:Antibodies; D005904:Glial Fibrillary Acidic Protein; D005680:gamma-Aminobutyric Acid