title: Localization of an arenavirus protein in the nuclei of infected cells.
abstract: Host cell nuclear involvement in an arenavirus infection was examined by immunofluorescence. Both polyclonal antisera and monoclonal antibodies specific for the major nucleocapsid (N) polypeptide revealed virus-specific nuclear inclusions in Pichinde virus-infected Vero cells. Immunoprecipitation of infected cell extracts with the anti-N monoclonal antibodies and subsequent analysis by SDS-PAGE, identified two N-related proteins with mol. wt. of 36,000 (p36) and 28,000 (p28) in addition to the N polypeptide. Only those monoclonal antibodies which precipitated p28 as well as N and p36 were found to produce nuclear as well as cytoplasmic fluorescence. These findings suggest that either the p28 protein itself or a conformational variant of N was the nuclear antigen detected.
authors.affiliation: Department of Medical Microbiology, London School of Hygiene and Tropical Medicine, U.K.
authors.forename: P R
authors.initials: PR
authors.lastname: Young
authors.forename: A C
authors.initials: AC
authors.lastname: Chanas
authors.forename: S R
authors.initials: SR
authors.lastname: Lee
authors.forename: E A
authors.initials: EA
authors.lastname: Gould
authors.forename: C R
authors.initials: CR
authors.lastname: Howard
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D001116:Arenaviridae / Q000235:genetics* / Q000648:ultrastructure; D002467:Cell Nucleus / Q000382:microbiology* / Q000648:ultrastructure; D002472:Cell Transformation, Viral; D003593:Cytoplasm / Q000382:microbiology* / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D006801:Humans; D008970:Molecular Weight; D014709:Vero Cells; D014764:Viral Proteins / Q000032:analysis*
chemical_list: D000911:Antibodies, Monoclonal; D014764:Viral Proteins