Effect on rat embryos of in vitro culture in sera from human diabetic patients.
Recently conditions have been established whereby rat embryos can be cultured in vitro in human serum over the period of their major morphogenesis. By standardizing the amount of glucose in sera from diabetic subjects prior to culture, it has been possible to investigate factors other than hyperglycemia which might account for the increased incidence of congenital defects seen in infants of diabetic mothers. The incidence of abnormalities in rat embryos cultured in sera from chlorpropamide-treated diabetics was not significantly different from that using control sera from normal subjects. On the other hand, sera from insulin-dependent diabetics produced more defects in rat embryos than sera from either normal subjects or diabetics on oral agents. The incidence of abnormal rat embryos does not appear to be correlated with patient age, with the original blood glucose concentration or with the concentration of hemoglobin A1 or, in the insulin group, with the total daily insulin dosage.
Moore
D C
DC
Department of Zoology, University of Liverpool, UK.
Stanisstreet
M
M
Clarke
C A
CA
Beck
F
F
Hipkin
L J
LJ
D000818:Animals; D002747:Chlorpropamide / Q000627:therapeutic use; D003470:Culture Media; D003922:Diabetes Mellitus, Type 1 / Q000097:blood* / Q000188:drug therapy; D003924:Diabetes Mellitus, Type 2 / Q000097:blood* / Q000188:drug therapy; D004622:Embryo, Mammalian / Q000166:cytology* / Q000648:ultrastructure; D006801:Humans; D007328:Insulin / Q000627:therapeutic use; D008855:Microscopy, Electron, Scanning; D009924:Organ Culture Techniques; D051381:Rats
D003470:Culture Media; D007328:Insulin; D002747:Chlorpropamide