Advances in the use of nucleic acid probes in diagnosis of viral diseases of man. Brief review.
A variety of methods are now available for the preparation and labelling of viral nucleic acids for use as probes in diagnostic virology. Some of these are assessed including the use of synthetic oligonucleotides in place of molecularly cloned nucleic acids, and alternatives to labelling with radioactive isotopes such as biotin, enzymes and fluorochromes. Dot blot, sandwich, indirect sandwich and in situ hybridization are covered, and examples given of the current use of nucleic acid probes in detection of human viral infections. The potential and limitations of nucleic acid hybridization are discussed in the light of these new methods.
Norval
M
M
Department of Bacteriology, University of Edinburgh Medical School.
Bingham
R W
RW
D004279:DNA, Viral / Q000032:analysis* / Q000235:genetics; D006801:Humans; D012367:RNA, Viral / Q000032:analysis* / Q000235:genetics; D014777:Virus Diseases / Q000175:diagnosis*; D014780:Viruses / Q000235:genetics
D004279:DNA, Viral; D012367:RNA, Viral