Differentiation of Shigella by esterase electrophoretic polymorphism.
The electrophoretic mobilities of four esterases (A, B, C, and I) of 182 strains of Shigella dysenteriae, S. flexneri, S. boydii and S. sonnei were compared to those of 636 strains of Escherichia coli from various origins, including the Alkalescens Dispar group and enteroinvasive strains. Discriminant analysis of the distribution of esterases among the strains revealed that Shigella could be distinguished from E. coli by differences in the distribution of allozymes of esterases C and I. Principal components analysis distinguished four major clusters of Shigella strains corresponding to the following: S. dysenteriae serotype 1; S. flexneri serotypes 1 to 5; S. flexneri serotype 6 and S. boydii serotypes 2 and 4; and S. sonnei. The last three were characterized by distinct electrophoretic variants of carboxylesterase B, as judged by the two-dimensional electrophoretic profile and titration curves. The distinct esterase pattern obtained for the strains of S. boydii serotype 13 substantiates the view that this serotype may constitute a new species.
Goullet
P
P
Laboratoire de Microbiologie, Faculté de Médecine Xavier Bichat (Université Paris VII), France.
Picard
B
B
D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000145:classification / Q000201:enzymology; D004950:Esterases / Q000145:classification*; D014644:Genetic Variation; D007525:Isoelectric Focusing; D011110:Polymorphism, Genetic; D012703:Serotyping; D012760:Shigella / Q000145:classification* / Q000201:enzymology
D004950:Esterases