Sensitive methods for determining subclasses of IgG anti-A and anti-B in sera of blood-group-O women with a blood-group-A or -B child.
The determination of the subclasses of IgG antibodies against blood groups A and B is important in order to improve our understanding and predict haemolytic disease of the newborn due to IgG anti-A or -B. We describe two techniques that circumvent the problem of the agglutination of A and B red cells by the corresponding IgG antibodies in saline: an antiglobulin consumption test and a modified solid-phase micro-immunofluorescence test. The results of the two techniques are compared with the results obtained in the indirect antiglobulin test beyond the saline agglutination titre in a microplate technique. The solid-phase micro-immunofluorescence test was the most sensitive for the determination of the subclasses of IgG anti-A and -B. Usually sera contained IgG2 anti-A, B in a higher titre than antibodies of other subclasses.
Brouwers
H A
HA
Overbeeke
M A
MA
Gemke
R J
RJ
Maas
C J
CJ
van Leeuwen
E F
EF
Engelfriet
C P
CP
D000017:ABO Blood-Group System / Q000276:immunology*; D003298:Coombs Test; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007074:Immunoglobulin G / Q000145:classification*; D007231:Infant, Newborn; D007518:Isoantibodies / Q000145:classification*; D011247:Pregnancy / Q000276:immunology*
D000017:ABO Blood-Group System; D007074:Immunoglobulin G; D007518:Isoantibodies