title: [Effectiveness of the promoter Ptac' in vitro and in mini-cells].
abstract: The efficiency of hybrid promotor Ptac', comprising a synthetic trp-promoter and lacUV5 "-10" sequence, was studied. By means of electrophoresis and hybridization of RNA-products obtained in vitro and in minicells, with promotor-containing plasmids; the hybrid promotor was found to be 6 times and 3-4 times as efficient as trp and lacUV5 promoters, respectively.
authors.forename: L Ia
authors.initials: LIa
authors.lastname: Denisova
authors.forename: S N
authors.initials: SN
authors.lastname: Zagrebel'nyĭ
authors.forename: A I
authors.initials: AI
authors.lastname: Zakabunin
authors.forename: E V
authors.initials: EV
authors.lastname: Kileva
authors.forename: V A
authors.initials: VA
authors.lastname: Kovzhov
mesh_terms: D004926:Escherichia coli / Q000235:genetics; D005818:Genetic Engineering; D007763:Lac Operon; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D011401:Promoter Regions, Genetic; D014364:Tryptophan / Q000235:genetics
chemical_list: D014364:Tryptophan
vernacular_title: Izuchenie éffektivnosti promotora Ptac' in vitro i v mini-kletkakh.