Induction of bovine plaque-forming cells in vitro.
A culture system was developed for the in vitro inoculation of bovine splenic lymphocytes with sheep RBC and trinitrophenylated proteins. The addition of pokeweed mitogen to the cultures was necessary for the induction of plaque-forming cells to the antigen. This culture system required the use of autologous serum and the addition of mitogen.
Kaattari
S L
SL
Nevin
B D
BD
D000818:Animals; D002417:Cattle / Q000502:physiology*; D002478:Cells, Cultured; D005260:Female; D006462:Hemolytic Plaque Technique; D008214:Lymphocytes / Q000502:physiology*; D008297:Male; D013154:Spleen / Q000166:cytology*