title: Transcriptional control of yeast ribosomal protein synthesis during carbon-source upshift.
abstract: Shifting a yeast culture from an ethanol-based medium to a glucose-based medium causes a coordinate increase of the cellular levels of ribosomal protein mRNAs by about a factor 4 within 30 min. Making use of hybrid genes encompassing different portions of the 5'-flanking region of the L25-gene, we could show that the increase in mRNAs is a transcriptional event, mediated through DNA sequences upstream of the ribosomal protein (rp) genes. Further analysis revealed that sequence elements are involved that many rp-genes have in common and that previously were identified as transcription activation sites (RPG-boxes or UASrpg). Using appropriate deletion mutants of the fusion genes we could demonstrate that a single RPG-box is sufficient for the transcriptional upshift. In addition, both copy genes encoding rp28 which differ considerably in their extent of transcriptional activity, show the upshift effect in a proportional manner. Definite proof for the role of the UASrpg in nutritional regulation was obtained by examining the effect of a synthetic RPG-box on transcription.
authors.affiliation: Biochemisch Laboratorium, Vrije Universiteit, Amsterdam, The Netherlands.
authors.forename: M H
authors.initials: MH
authors.lastname: Herruer
authors.forename: W H
authors.initials: WH
authors.lastname: Mager
authors.forename: L P
authors.initials: LP
authors.lastname: Woudt
authors.forename: R T
authors.initials: RT
authors.lastname: Nieuwint
authors.forename: G M
authors.initials: GM
authors.lastname: Wassenaar
authors.forename: P
authors.initials: P
authors.lastname: Groeneveld
authors.forename: R J
authors.initials: RJ
authors.lastname: Planta
mesh_terms: D000431:Ethanol / Q000378:metabolism; D005656:Fungal Proteins / Q000096:biosynthesis; D005786:Gene Expression Regulation; D005947:Glucose / Q000378:metabolism; D005810:Multigene Family; D012331:RNA, Fungal / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid; D012269:Ribosomal Proteins / Q000096:biosynthesis*; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D005656:Fungal Proteins; D012331:RNA, Fungal; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D012269:Ribosomal Proteins; D000431:Ethanol; D005947:Glucose