title: The effect of neutrophil migration and prolonged neutrophil contact on epithelial permeability.
abstract: The effect of neutrophil migration and prolonged neutrophil contact on epithelial permeability was examined. Although neutrophil migration was not associated with a change in epithelial permeability, prolonged neutrophil-epithelial contact following migration resulted in an increase in epithelial permeability. These results were not altered by catalase, a specific neutrophil elastase inhibitor, methoxysuccinyl-Ala-Ala-Pro-Val-chloromethyl ketone or cyclohexamide. This suggests that neutrophil migration does not occur via an H2O2-induced reversible mechanism of junctional opening, which we describe herein.
authors.affiliation: Department of Medicine, National Immunology and Respiratory Medicine, Denver, CO 80206.
authors.forename: P E
authors.initials: PE
authors.lastname: Parsons
authors.forename: K
authors.initials: K
authors.lastname: Sugahara
authors.forename: G R
authors.initials: GR
authors.lastname: Cott
authors.forename: R J
authors.initials: RJ
authors.lastname: Mason
authors.forename: P M
authors.initials: PM
authors.lastname: Henson
mesh_terms: D000818:Animals; D002460:Cell Line; D002465:Cell Movement; D002470:Cell Survival; D004553:Electric Conductivity; D004847:Epithelial Cells; D004848:Epithelium / Q000502:physiology; D005455:Fluorescent Antibody Technique; D006861:Hydrogen Peroxide / Q000494:pharmacology; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D008564:Membrane Potentials; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D010196:Pancreatic Elastase / Q000378:metabolism; D010539:Permeability; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D006861:Hydrogen Peroxide; D010196:Pancreatic Elastase