title: Interaction of GAL4 and GAL80 gene regulatory proteins in vitro.
abstract: The GAL80 protein of Saccharomyces cerevisiae, synthesized in vitro, bound tightly to GAL4 protein and to a GAL4 protein-upstream activation sequence DNA complex, as shown by (i) coimmunoprecipitation of GAL4 and GAL80 proteins with anti-GAL4 antiserum, (ii) an electrophoretic mobility shift of a GAL4 protein-upstream activation sequence DNA complex upon the addition of GAL80 protein, and (iii) GAL4-dependent binding of GAL80 protein to upstream activation sequence DNA immobilized on Sepharose beads. Anti-GAL4 antisera were raised against a GAL4-URA3 fusion protein, which could be purified to homogeneity in a single step with the use of an affinity chromatographic procedure for the URA3 gene product.
authors.affiliation: Department of Cell Biology, Stanford University School of Medicine, California 94305.
authors.forename: N F
authors.initials: NF
authors.lastname: Lue
authors.forename: D I
authors.initials: DI
authors.lastname: Chasman
authors.forename: A R
authors.initials: AR
authors.lastname: Buchman
authors.forename: R D
authors.initials: RD
authors.lastname: Kornberg
mesh_terms: D004271:DNA, Fungal / Q000235:genetics; D004268:DNA-Binding Proteins / Q000502:physiology*; D005656:Fungal Proteins / Q000502:physiology; D005690:Galactose / Q000502:physiology*; D005786:Gene Expression Regulation; D007158:Immunologic Techniques; D046911:Macromolecular Substances; D011485:Protein Binding; D011993:Recombinant Fusion Proteins / Q000276:immunology; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014157:Transcription Factors / Q000502:physiology*
chemical_list: D004271:DNA, Fungal; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D046911:Macromolecular Substances; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors; D005690:Galactose