Structure of Escherichia coli capsular antigen K34.
The structure of the heat-stable K antigen of Escherichia coli serotype K34 has been determined by n.m.r. spectroscopy, methylation analysis, and Smith degradation to be (formula; see text) The assignment of the single alpha-linkage to the D-glucosyl residue was confirmed by the positive reaction of an alpha-D-glucosidase on the oligosaccharide obtained by bacteriophage depolymerization. It is noteworthy that the same enzyme was without action on the native polysaccharide.
Dutton
G G
GG
Department of Chemistry, University of British Columbia, Vancouver, Canada.
Kuma-Mintah
A
A
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D003090:Coliphages / Q000378:metabolism; D004926:Escherichia coli / Q000276:immunology*; D009682:Magnetic Resonance Spectroscopy; D011135:Polysaccharides, Bacterial / Q000302:isolation & purification*
D011135:Polysaccharides, Bacterial