Acylation of glycerol 3-phosphate is the sole pathway of de novo phospholipid synthesis in Escherichia coli.
The inhibition of phospholipid synthesis engendered by starving glycerol 3-phosphate (G3P) auxotrophs of Escherichia coli (plsB or gpsA) for G3P is incomplete; 5 to 10% of the normal rate of phospholipid synthesis remains, even after prolonged starvation. We report that G3P starvation of a strain having lesions in both the gpsA and plsB genes resulted in essentially complete (greater than 98.5%) inhibition of phospholipid synthesis, indicating that all de novo glycerolipid synthesis in E. coli proceeds by acylation of G3P.
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D000085:Acetates / Q000378:metabolism; D000215:Acylation; D004075:Diglycerides / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism*; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D005994:Glycerophosphates / Q000378:metabolism*; D010712:Phosphatidic Acids / Q000096:biosynthesis; D010743:Phospholipids / Q000096:biosynthesis*
D000085:Acetates; D004075:Diglycerides; D005994:Glycerophosphates; D010712:Phosphatidic Acids; D010743:Phospholipids; C029620:alpha-glycerophosphoric acid; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D005990:Glycerol