title: 31P NMR saturation-transfer measurements in Saccharomyces cerevisiae: characterization of phosphate exchange reactions by iodoacetate and antimycin A inhibition.
abstract: 31P nuclear magnetic resonance (NMR) saturation-transfer (ST) techniques have been used to measure steady-state flows through phosphate-adenosine 5'-triphosphate (ATP) exchange reactions in glucose-grown derepressed yeast. Our results have revealed that the reactions catalyzed by glyceraldehyde-3-phosphate dehydrogenase/phosphoglycerate kinase (GAPDH/PGK) and by the mitochondrial ATPase contribute to the observed ST. Contributions from these reactions were evaluated by performing ST studies under various metabolic conditions in the presence and absence of either iodoacetate, a specific inhibitor of GAPDH, or the respiratory chain inhibitor antimycin A. Intracellular phosphate (Pi) longitudinal relaxation times were determined by performing inversion recovery experiments during steady-state ATP gamma saturation and were used in combination with ST data to determine Pi consumption rates. 13C NMR and O2 electrode measurements were also conducted to monitor changes in rates of glucose consumption and O2 consumption, respectively, under the various metabolic conditions examined. Our results suggest that GAPDH/PGK-catalyzed Pi-ATP exchange is responsible for antimycin-resistant saturation transfer observed in anaerobic and aerobic glucose-fed yeast. Kinetics through GAPDH/PGK were found to depend on metabolic conditions. The coupled system appears to operate in a unidirectional manner during anaerobic glucose metabolism and bidirectionally when the cells are respiring on exogenously supplied ethanol. Additionally, mitochondrial ATPase activity appears to be responsible for the transfer observed in iodoacetate-treated aerobic cells supplied with either glucose or ethanol, with synthesis of ATP occurring unidirectionally.
authors.affiliation: Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06511.
authors.forename: S L
authors.initials: SL
authors.lastname: Campbell-Burk
authors.forename: K A
authors.initials: KA
authors.lastname: Jones
authors.forename: R G
authors.initials: RG
authors.lastname: Shulman
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D000332:Aerobiosis; D000693:Anaerobiosis; D000968:Antimycin A / Q000494:pharmacology*; D006019:Glycolysis; D007461:Iodoacetates / Q000494:pharmacology*; D019807:Iodoacetic Acid; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D010101:Oxygen Consumption / Q000187:drug effects; D010710:Phosphates / Q000378:metabolism*; D010758:Phosphorus; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000378:metabolism*; D013403:Sugar Phosphates / Q000378:metabolism
chemical_list: D007461:Iodoacetates; D010710:Phosphates; D013403:Sugar Phosphates; D010758:Phosphorus; D000968:Antimycin A; D000255:Adenosine Triphosphate; D019807:Iodoacetic Acid