DNA probes for identification of enteroinvasive Escherichia coli.
Eighty-one Escherichia coli strains belonging to all known invasive O serogroups were tested with two distinct invasiveness probes (pMR17 and pSF55). All 54 Sereny test-positive strains and 5 strains that lost Sereny positivity during storage hybridized with both probes. Probe-positive strains carried a 120- to 140-megadalton plasmid, did not produce lysine decarboxylase, and, with the exception of certain serotypes, were nonmotile. Motile strains of serotype O144:H25 were for the first time characterized as invasive by hybridization with the probes.
Gomes
T A
TA
Department of Microbiology, Immunology, and Parasitology, Escola Paulista de Medicina, Sao Paulo, Brazil.
Toledo
M R
MR
Trabulsi
L R
LR
Wood
P K
PK
Morris
J G
JG
D004269:DNA, Bacterial / Q000032:analysis*; D004926:Escherichia coli / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D006367:HeLa Cells; D006801:Humans; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010957:Plasmids
D004269:DNA, Bacterial