Anti-Purkinje cell antibody producing B-cell lines from a patient with paraneoplastic cerebellar degeneration.
Two patients with paraneoplastic cerebellar degeneration with anti-Purkinje cell antibodies are described. Using an avidin-biotin peroxidase complex method, serum anti-Purkinje cell antibody titers of these patients were 1:32,000 and 1:64,000, and the cerebrospinal fluid titers were 1:40 and 1:320, respectively. After extensive plasmapheresis together with radiation therapy and chemotherapy, the cerebellar symptoms of one patient ameliorated concomitantly with a reduced serum anti-Purkinje cell antibody titer. Four B-cell lines producing anti-Purkinje cell antibodies were established from one patient by Epstein-Barr virus transformation of peripheral lymphocytes. None of these antibodies reacted with peripheral T cells, although the serum anti-Purkinje cell activity of the patient was markedly reduced after absorption with purified human T cells. Thus, anti-Purkinje cell antibodies are apparently directed both to antigens unique to the cerebellar tissue and those shared by T cells.
Tsukamoto
T
T
Yoshie
O
O
Tada
K
K
Iwasaki
Y
Y
D000230:Adenocarcinoma / Q000150:complications / Q000628:therapy; D001323:Autoantibodies / Q000032:analysis*; D001402:B-Lymphocytes / Q000276:immunology*; D002460:Cell Line; D002526:Cerebellar Diseases / Q000209:etiology / Q000276:immunology*; D002555:Cerebrospinal Fluid / Q000276:immunology; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007124:Immunoenzyme Techniques; D008875:Middle Aged; D010051:Ovarian Neoplasms / Q000150:complications / Q000628:therapy; D010257:Paraneoplastic Syndromes / Q000276:immunology*; D010956:Plasmapheresis; D011689:Purkinje Cells / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology; D014594:Uterine Neoplasms / Q000150:complications / Q000628:therapy
D001323:Autoantibodies