Studies on the differentiation of the human myelomonocytic cell line RC-2A in response to lymphocyte-derived factors.
Cells of the human myelomonocytic line RC-2A were induced to differentiate toward macrophages by culturing for up to 12 days in the presence of supernatant from phytohaemagglutinin stimulated human peripheral blood mononuclear cells (PHA-LCM). The process of differentiation was monitored by changes in expression of two-macrophage related enzymes (alpha-naphthol butyrate esterase and acid phosphatase), the changes in expression of the monocyte-macrophage cell surface markers detected by the monoclonal antibodies anti-Mo1 and anti-Mo2, HLA class 2 antigen detected by FMC-14, and alteration in cell morphology. Maturation induced by PHA-LCM was accompanied by a marked decrease in the proliferative potential of the cell population, and a reduced ability to form colonies in semi-solid medium. Induced RC-2A cells were able to stimulate in one-way mixed leukocyte culture more effectively than control cells.
Lyons
A B
AB
Department of Microbiology and Immunology, University of Adelaide, South Australia.
Ashman
L K
LK
D000954:Antigens, Surface / Q000032:analysis; D002454:Cell Differentiation; D002455:Cell Division; D003470:Culture Media; D005455:Fluorescent Antibody Technique; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D006651:Histocytochemistry; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000201:enzymology / Q000473:pathology*; D008214:Lymphocytes / Q000502:physiology*; D010835:Phytohemagglutinins / Q000494:pharmacology; D011961:Receptors, Fc / Q000378:metabolism; D014407:Tumor Cells, Cultured / Q000473:pathology
D000954:Antigens, Surface; D003470:Culture Media; D000949:Histocompatibility Antigens Class II; D010835:Phytohemagglutinins; D011961:Receptors, Fc