[Expression of human leukocyte interferon type A in Saccharomyces cerevisiae under the control of regulatory elements of the yeast gene URA3].
Expression of a chromosomal gene for human leucocyte interferon A was obtained due to interaction of 5'-nontranslating region of a cloned interferon gene with the regulatory sequences from UNA3 yeast gene. The sequence of 5'-nontranslating region from interferon gene essential for its expression in yeast is localized within 130 b p. from the initiating codon. Due to increasing of plasmid stability and copy number a 60-fold increase. in interferon yield was obtained in yeast transformants reaching the level of 6 X 10(7) u X 1(-1). The data are presented supposing the existence of functional polycistronic mRNA in yeast.
Nikoshkov
A B
AB
Zlochevskiĭ
M L
ML
Naroditskaia
V A
VA
Eremashvili
M R
MR
Tolstorukov
I I
II
D001483:Base Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005786:Gene Expression Regulation; D005800:Genes, Fungal; D007370:Interferon Type I / Q000235:genetics*; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D007370:Interferon Type I; D011994:Recombinant Proteins
Ekspressiia gena leĭkotsitarnogo interferona A cheloveka v drozhzhakh Saccharomyces cerevisiae pod kontrolem reguliatornykh élementov drozhzhevogo gena URA3.