Production of metalloproteinase in gastric tissue with acetic acid-induced ulcer or taurocholic acid-induced gastritis.
A metalloproteinase was extracted from a medium of cultured gastric tissues following acetic acid-induced ulcer or taurocholic acid-induced gastritis, and the enzyme was partially purified by Sephadex G-200 column chromatography. This proteinase was released in active and latent forms with apparent molecular weights of 170,000 and 210,000, respectively. Its activity was abolished by treatment with ethylenediaminetetraacetic acid or 1,10-phenanthroline, but not with phenylmethylsulfonyl fluoride or soybean trypsin inhibitor. This enzyme degraded type I gelatin and type IV collagen but did not attack type I collagen. In contrast, very little of this enzyme was released from gastric tissue following erosion caused by water-immersion stress or from normal gastric tissue. These results suggest that this metalloproteinase plays an important role in gastric lesions by breaking down the basement membrane.
Nakanishi
J
J
Murakami
S
S
Ishikura
Y
Y
Tsuchiya
S
S
Mori
Y
Y
D000085:Acetates; D019342:Acetic Acid; D000818:Animals; D003094:Collagen / Q000378:metabolism; D010450:Endopeptidases / Q000096:biosynthesis*; D005756:Gastritis / Q000139:chemically induced / Q000201:enzymology*; D005780:Gelatin / Q000378:metabolism; D008297:Male; D008666:Metalloendopeptidases; D011480:Protease Inhibitors; D051381:Rats; D011919:Rats, Inbred Strains; D013270:Stomach / Q000201:enzymology*; D013276:Stomach Ulcer / Q000139:chemically induced / Q000201:enzymology*; D013656:Taurocholic Acid
D000085:Acetates; D011480:Protease Inhibitors; D013656:Taurocholic Acid; D005780:Gelatin; D003094:Collagen; D010450:Endopeptidases; D008666:Metalloendopeptidases; D019342:Acetic Acid