title: Production and characterization of monoclonal antibodies to 17 alpha-hydroxyprogesterone.
abstract: Hybridoma clones producing antibodies to 17 alpha-hydroxyprogesterone (17-OHP) were established by using a 17-OHP-bovine serum albumin conjugate as an immunogen. Six representative IgG-class monoclonal antibodies of high affinity (10(8)-10(9) M-1) showed differential reactivities with several structurally related steroids. Two enzyme immunoassay (EIA) systems (fluorescence EIA and micro-EIA) for 17-OHP using OHP 4B2.2.3, which showed the lowest cross-reactivity with other steroids, were established. The micro-EIA system was shown to be applicable to the mass-screening of congenital adrenal hyperplasia.
authors.affiliation: Division of Radiochemistry, National Institute of Hygienic Sciences, Tokyo, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Sawada
authors.forename: T
authors.initials: T
authors.lastname: Terao
authors.forename: S
authors.initials: S
authors.lastname: Itoh
authors.forename: M
authors.initials: M
authors.lastname: Maeda
authors.forename: A
authors.initials: A
authors.lastname: Tsuji
authors.forename: H
authors.initials: H
authors.lastname: Hosoda
authors.forename: T
authors.initials: T
authors.lastname: Nambara
mesh_terms: D019326:17-alpha-Hydroxyprogesterone; D000312:Adrenal Hyperplasia, Congenital / Q000097:blood; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D000941:Antigens / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006825:Hybridomas / Q000276:immunology; D006908:Hydroxyprogesterones / Q000097:blood / Q000276:immunology*; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000276:immunology; D008403:Mass Screening; D051379:Mice; D008807:Mice, Inbred BALB C; D012710:Serum Albumin, Bovine / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D000941:Antigens; D006908:Hydroxyprogesterones; D007074:Immunoglobulin G; D012710:Serum Albumin, Bovine; D019326:17-alpha-Hydroxyprogesterone