title: Bovine mitochondrial ribosomes: effect of cations and heterologous dissociation factors on subunit interactions.
abstract: The effects of cations and ribosome dissociation factors on the equilibrium between the bovine mitochondrial ribosome and its subunits has been investigated. As observed with other ribosomes, Mg2+ ions promote subunit association while monovalent cations promote subunit dissociation. E. coli IF-3 will prevent the reassociation of mitochondrial 28 S and 39 S subunits. However, at least 5-fold higher concentrations of IF-3 are required with mitochondrial subunits than are required with bacterial subunits. The cytoplasmic factor eIF-6, has no detectable activity in preventing mitochondrial ribosomal subunit association.
authors.affiliation: Department of Chemistry, University of North Carolina, Chapel Hill 27514.
authors.forename: L
authors.initials: L
authors.lastname: Spremulli
authors.forename: B L
authors.initials: BL
authors.lastname: Kraus
mesh_terms: D002412:Cations / Q000494:pharmacology; D004926:Escherichia coli; D008274:Magnesium / Q000494:pharmacology; D008930:Mitochondria, Liver / Q000378:metabolism / Q000648:ultrastructure*; D010442:Peptide Chain Initiation, Translational; D010443:Peptide Chain Termination, Translational; D010448:Peptide Initiation Factors / Q000494:pharmacology; D010454:Peptide Termination Factors / Q000378:metabolism*; D011188:Potassium / Q000494:pharmacology; D012270:Ribosomes / Q000378:metabolism* / Q000648:ultrastructure; D013096:Spermine / Q000494:pharmacology; D014908:Triticum
chemical_list: D002412:Cations; D010448:Peptide Initiation Factors; D010454:Peptide Termination Factors; D013096:Spermine; D008274:Magnesium; D011188:Potassium