Interleukin-HP1-related hybridoma and plasmacytoma growth factors induced by lipopolysaccharide in vivo.
Serum of lipopolysaccharide (LPS)-treated mice was found to support the growth of interleukin-HP1 (HP1)-dependent hybridoma and plasmacytoma cell lines. This growth-promoting activity, which was undetectable in normal serum, rose more than 1000-fold within 2 h after i.v. injection of the toxin and disappeared in less than 1 day. It could be traced to a single component, which behaved like HP1 in sodium dodecyl sulfate-polyacrylamide gel electrophoresis and reverse phase high performance liquid chromatography, and was completely inhibited by a rabbit anti-HP1 antiserum. The LPS-induced factor was apparently not of T cell origin, as indicated by the strong activity found in the serum of LPS-treated nude mice. In vitro, LPS also considerably enhanced the production by macrophages of a factor with similar characteristics.
Coulie
P G
PG
Cayphas
S
S
Vink
A
A
Uyttenhove
C
C
Van Snick
J
J
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D006133:Growth Substances / Q000302:isolation & purification / Q000502:physiology*; D006825:Hybridomas / Q000473:pathology*; D007158:Immunologic Techniques; D015850:Interleukin-6; D008070:Lipopolysaccharides / Q000276:immunology*; D008222:Lymphokines / Q000032:analysis / Q000502:physiology*; D008264:Macrophages / Q000502:physiology; D051379:Mice; D008819:Mice, Nude / Q000502:physiology; D015846:Monokines; D010954:Plasmacytoma / Q000473:pathology*; D011506:Proteins / Q000502:physiology
D006133:Growth Substances; D015850:Interleukin-6; D008070:Lipopolysaccharides; D008222:Lymphokines; D015846:Monokines; D011506:Proteins