The in vitro cultivation of P. falciparum ookinetes, and their enrichment on Nycodenz density gradients.
Employing a simple method of growing ookinetes of Plasmodium falciparum in culture, 40% of mature gametocytes convert to macrogametes, 4% reach the retort-form ookinete stage and 0.45% become mature ookinetes. A single-step gradient centrifugation method on 12.5% Nycodenz enriches both gametocytes and ookinetes.
Carter
E H
EH
Department of Pure and Applied Biology, Imperial College, London.
Suhrbier
A
A
Beckers
P J
PJ
Sinden
R E
RE
D000818:Animals; D002499:Centrifugation, Density Gradient; D004912:Erythrocytes / Q000469:parasitology; D007472:Iohexol; D009024:Morphogenesis; D010963:Plasmodium falciparum / Q000166:cytology / Q000254:growth & development*
D007472:Iohexol