title: Purification and partial amino acid sequencing of rat bone tumor (UMR106) alkaline phosphatase.
abstract: Cultured rat osteosarcoma (UMR106) alkaline phosphatase was purified to apparent homogeneity by sequential application of polyclonal antibody affinity, DEAE-cellulose, and Sepharose CL-6B chromatography. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the enzyme preparation treated with sodium dodecyl sulfate and mercaptoethanol showed the presence of a dominant band (using silver staining) corresponding to a molecular weight of 80,000. The amino acid composition was similar to those of various alkaline phosphatases. The N-terminal amino acid sequence was determined as follows: Phe-Val-Pro-Glu-Lys-Glu-Lys- Asp-Pro-Ser-Tyr-Trp-Arg-Gln-Gln-Ala-Gln-Glu-Thr-Leu- Lys-Asn-Ala-Leu-Lys-?-Gln-Lys-?-Asn-Val-Asn-Ala-Lys.
authors.forename: H H
authors.initials: HH
authors.lastname: Hsu
authors.forename: J
authors.initials: J
authors.lastname: Rouse
authors.forename: J
authors.initials: J
authors.lastname: Hamilton
authors.forename: H C
authors.initials: HC
authors.lastname: Anderson
mesh_terms: D000469:Alkaline Phosphatase / Q000302:isolation & purification*; D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001859:Bone Neoplasms / Q000201:enzymology*; D002845:Chromatography / Q000379:methods; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D007158:Immunologic Techniques; D012516:Osteosarcoma / Q000201:enzymology*; D051381:Rats; D012513:Sarcoma, Experimental / Q000201:enzymology*
chemical_list: D000596:Amino Acids; D000469:Alkaline Phosphatase