Immunochemical comparison of peptide angiogenic factors.
A panel of 40 monoclonal antibodies was constructed in response to cationic endothelial cell growth factor (c-ECGF), the cationic peptide mitogen isolated from endothelial mitogen. The monoclonal antibodies were assayed by dot blot for immunoreactivity to various other peptide angiogenic factors. The panel of monoclonal antibodies to c-ECGF exhibited complete cross-reactivity with pituitary fibroblast growth factor and sarcoma-derived growth factor. A group of 28 monoclonal antibodies was found to exhibit reactivity to anionic endothelial mitogen (a-ECGF), brain fibroblast growth factor, endothelial cell growth factor, and retina-derived growth factor. None of the monoclonal antibodies was found to react with epidermal growth factor or platelet-derived growth factor. These data provide an immunological basis for grouping heparin-binding endothelial cell growth factors into anionic and cationic groups.
Schelling
M E
ME
Hawker
J R
JR
Granger
H J
HJ
D000818:Animals; D000911:Antibodies, Monoclonal; D002417:Cattle; D003429:Cross Reactions; D016228:Endothelial Growth Factors; D004727:Endothelium / Q000166:cytology* / Q000187:drug effects; D004797:Enzyme-Linked Immunosorbent Assay; D006133:Growth Substances / Q000032:analysis* / Q000276:immunology / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D016228:Endothelial Growth Factors; D006133:Growth Substances