Prostacyclin immobilized albuminated surfaces.
The adhered platelets on artificial surfaces play a crucial role in inducing thrombosis. Therefore, it is very much desirable to have surfaces which can effectively retard platelet adhesion and aggregation. Prostaglandins like PGE2, PGE1 and PGD2 possess potent antiplatelet activity, but have very short half life. A chemically stable prostacyclin analog, 10,10-difluoro-13-dehydroprostacyclin (DF2-PGI2) seems to be promising. Polyelectrolyte (PE) synthesized from natural poly cis-1,4 isoprene has also been found to possess outstanding anticoagulant and antiplatelet activity. Modification of polyetherurethane urea (PEUU) by immobilizing DF2-PGI2 and poly-electrolyte in various combinations using glow discharge technique has been attempted. Surfaces thus modified showed negligible platelet adherence. The inhibition of platelet adherence in presence of inducing agents like fibrinogen, thrombin and ADP was also remarkable. The interactions of albumin and fibrinogen with the modified surfaces were studied using 125I labelled proteins.
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D000244:Adenosine Diphosphate / Q000494:pharmacology; D000327:Adsorption; D000418:Albumins; D000818:Animals; D001672:Biocompatible Materials; D002417:Cattle; D004573:Electrolytes; D011464:Epoprostenol / Q000031:analogs & derivatives; D005340:Fibrinogen / Q000494:pharmacology; D007700:Kinetics; D010973:Platelet Adhesiveness / Q000187:drug effects; D011140:Polyurethanes; D011465:Prostaglandins, Synthetic; D013499:Surface Properties; D013917:Thrombin / Q000494:pharmacology; D014508:Urea
D000418:Albumins; D001672:Biocompatible Materials; D004573:Electrolytes; D011140:Polyurethanes; D011465:Prostaglandins, Synthetic; D000244:Adenosine Diphosphate; C024526:10,10-difluoro-13-dehydroprostacyclin; D014508:Urea; D005340:Fibrinogen; C044690:polyetherurethane urea; D011464:Epoprostenol; D013917:Thrombin