In vivo determination of modulated migration of radiolabeled leukocytes.
A method to determine migration of phagocytic cells in vivo has been evaluated. It was used to analyze leukocyte migration during ischemia, peritonitis, and after pretreatment with methylprednisolone. Phagocytic cells were labeled in vitro with 150 megabecquerel (4.05 mCi) of technetium Tc 99m colloid and reinfused. Saline 0.03 mg and 3.0 mg of endotoxin per kilogram per rat were injected subcutaneously in duplicate. Four hours later, a standardized amount of skin and subcutaneous tissue from the area of injection was removed and weighed. A dose-related accumulation of radiolabeled leukocytes to endotoxin was found in healthy controls but not in animals with hypoperfusion or peritonitis or those pretreated with methylprednisolone. This in vivo method to calculate leukocyte migration will give reproducible results with a mean difference between duplicate samples of less than 6%.
Bengtson
A
A
Hansson
G K
GK
Heideman
M
M
D000818:Animals; D002465:Cell Movement; D004731:Endotoxins / Q000008:administration & dosage; D004926:Escherichia coli; D007422:Intestines / Q000098:blood supply; D007511:Ischemia / Q000276:immunology; D007962:Leukocytes / Q000187:drug effects / Q000276:immunology*; D008070:Lipopolysaccharides / Q000008:administration & dosage; D008297:Male; D008775:Methylprednisolone / Q000494:pharmacology; D010538:Peritonitis / Q000276:immunology; D051381:Rats; D011919:Rats, Inbred Strains; D013668:Technetium Tc 99m Aggregated Albumin
D004731:Endotoxins; D008070:Lipopolysaccharides; D013668:Technetium Tc 99m Aggregated Albumin; D008775:Methylprednisolone