The effect of axotomy and denervation on calmodulin content in the superior cervical sympathetic ganglion of the rat.
Calmodulin (CaM) in the superior cervical sympathetic ganglion (SCG) of the rat and its changes after transection of the pre- or postganglionic nerves were studied biochemically and immunohistochemically. The concentration of CaM in the SCG was assayed using the extent of activation of CaM-dependent adenylate cyclase from bovine neural retina. In the SCG, CaM concentration was 4.5 micrograms/mg protein, a level similar to that in another peripheral ganglion, the nodose ganglion (4.9 micrograms/mg protein). Following denervation of the SCG, the total amount of ganglionic CaM did not change significantly within a week. On the other hand, CaM content per ganglion increased gradually 3 days after axotomy. Immunohistochemical examination revealed that CaM in unoperated SCG is present both in the neuronal fibers and in the cell soma of sympathetic neurons. But the intensity of the staining with CaM-antibody in the cytoplasmic soma varied among cells. The gross profile of the CaM immunostaining seemed to be unchanged one week after either denervation or axotomy, but the immunoelectron microscopic study showed heavy staining of the innercellular membranes of irregular shape in the axotomized SCG.
Seto-Ohshima
A
A
Sano
M
M
Kitajima
S
S
Kawamura
N
N
Yamazaki
Y
Y
Nagata
Y
Y
D000262:Adenylyl Cyclases / Q000378:metabolism; D000818:Animals; D001338:Autonomic Fibers, Postganglionic / Q000502:physiology*; D001339:Autonomic Fibers, Preganglionic / Q000502:physiology*; D001369:Axons / Q000378:metabolism / Q000502:physiology; D002147:Calmodulin / Q000378:metabolism*; D003714:Denervation; D005260:Female; D005728:Ganglia, Sympathetic / Q000378:metabolism* / Q000648:ultrastructure; D006651:Histocytochemistry; D007124:Immunoenzyme Techniques; D066298:In Vitro Techniques; D008297:Male; D008854:Microscopy, Electron; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
D002147:Calmodulin; D000262:Adenylyl Cyclases