A novel fibrillar structure in cultured cells detected by a monoclonal antibody.
Using a monoclonal antibody, we have detected an antigen present in a unique fibrillar structure in the cytoplasm of cultured cells by immunofluorescence. These structures have been identified by transmission electron microscopy and ultrastructural immunocytochemistry as large single paracrystalline arrays of individual filaments morphologically similar to intermediate filaments. The antibody detects these structures in fibroblastic and epithelioid cultured cell lines of mouse, rat, bovine, and human origin but not of avian origin. Only a small percentage of the cells in a culture contains these structures; each cell usually contains only one, although two or more have been observed in a single cell. The structures are elongated vermiform arrays of filaments in the cytoplasm (approximately 0.5 X 3 microns) which have a thread-like or toroidal appearance. Because of this shape, we have named the putative antigen recognized by this antibody "nematin." Double-label experiments showed that these structures had no relationship to tubulin or vimentin. Immunocytochemical localization in human tissues revealed a high concentration of a reactive antigen in the stratum granulosum of skin and in what probably are neuroglial cells in the central nervous system. This monoclonal antibody may detect a novel intermediate filament protein and/or a shared determinant of different intermediate filament proteins.
Willingham
M C
MC
Richert
N D
ND
Rutherford
A V
AV
D000818:Animals; D000911:Antibodies, Monoclonal; D000941:Antigens / Q000276:immunology; D002417:Cattle; D002460:Cell Line; D002645:Chickens; D003593:Cytoplasm / Q000648:ultrastructure*; D005455:Fluorescent Antibody Technique; D006651:Histocytochemistry; D006801:Humans; D007124:Immunoenzyme Techniques; D007382:Intermediate Filaments / Q000648:ultrastructure; D051379:Mice; D008854:Microscopy, Electron; D051381:Rats; D013045:Species Specificity; D013859:Thiocarbamates / Q000032:analysis* / Q000276:immunology; D014404:Tubulin / Q000032:analysis; D014746:Vimentin / Q000032:analysis
D000911:Antibodies, Monoclonal; D000941:Antigens; D013859:Thiocarbamates; D014404:Tubulin; D014746:Vimentin; C008435:methyldithiocarbamate