title: Identification and characterization of an antigen specific for normal erythroid precursor cells and its application in diagnosis of erythroleukemia.
abstract: A monoclonal antibody (designated K:1-6F) generated by hybridization of mouse myeloma cells with spleen cells from mice immunized with the erythroleukemic cell line K562 was found by fluorescence-activated cell sorter analysis, dot-blot assay and electroimmunoblotting to bind to a majority of cells in the K562 and HEL erythroleukemic cell lines, to a subset of cells of the erythroid lineage from normal bone marrow, to a subset of cells in all analysed cases (total 10) of erythroleukemia, and weakly to cells from patients with myeloid leukemia. The antibody did not bind to normal erythrocytes, monocytes, T- and B lymphocytes or granulocytes, as well as a panel of human malignant cell lines of hemopoietic origin (HL60, U937, Daudi, Molt-3, RH-L4 and U266). Biochemical characterization of the antigen defined by the antibody suggests that eht epitope is defined by a carbohydrate structure alone or in combination with proteins. Four molecules with Mr 100 kD, 65 kD, 45 kD and 18 kD respectively were immunoprecipitated from Triton X-100 extract of K562 erythroleukemia cells. Neuraminidase did not affect the binding of the antibody, whereas tunicamycin reduced the K:1-6F expression. The K:1-6F Mab was in normal bone marrow found to be specific for erythroid precursor cells and may therefore be useful in examination of normal and leukemic erythropoiesis.
authors.affiliation: Cancer Biology Laboratory, State University Hospital of Copenhagen, Denmark.
authors.forename: R B
authors.initials: RB
authors.lastname: Andreasen
authors.forename: L
authors.initials: L
authors.lastname: Olsson
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000943:Antigens, Differentiation / Q000032:analysis* / Q000276:immunology; D003114:Colony-Forming Units Assay; D004591:Electrophoresis, Polyacrylamide Gel; D004921:Erythropoietin / Q000032:analysis / Q000276:immunology; D006412:Hematopoietic Stem Cells / Q000276:immunology*; D006801:Humans; D007118:Immunoassay; D007124:Immunoenzyme Techniques; D007163:Immunosorbent Techniques; D004915:Leukemia, Erythroblastic, Acute / Q000175:diagnosis* / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009439:Neuraminidase / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000276:immunology; D014415:Tunicamycin / Q000494:pharmacology
chemical_list: D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation; D014415:Tunicamycin; D004921:Erythropoietin; D009439:Neuraminidase