title: Regulation of c-myc transcription and protein expression during activation of normal human B cells.
abstract: The close link between an actively expressed c-myc gene locus and cellular proliferation has been established in a variety of cell types. By using normal human peripheral blood B cells as a model for in vivo quiescence, we have assessed the expression pattern of the c-myc gene in terms of transcriptional activation and concurrent production of c-myc protein, following induction by antibodies directed against antigens on the cell membrane. Both the 1F5 monoclonal antibody, which reacts with the pan B cell antigen CD20, and the anti-mu could promote transcriptional activation of the c-myc gene roughly corresponding to the increased levels of cytoplasmic c-myc mRNAs. In addition, more than 80% of the B cells could be induced to express c-myc protein by either stimulus. Since treatment only with polyclonal anti-mu renders the B cells competent to proliferate in the presence of BCGF, c-myc protein expression is not per se sufficient for cell cycle progression into S phase.
authors.affiliation: Department of Pathology, Norwegian Radium Hospital, Montebello, Oslo.
authors.forename: E B
authors.initials: EB
authors.lastname: Smeland
authors.forename: K
authors.initials: K
authors.lastname: Beiske
authors.forename: B
authors.initials: B
authors.lastname: Ek
authors.forename: R
authors.initials: R
authors.lastname: Watt
authors.forename: S
authors.initials: S
authors.lastname: Pfeifer-Ohlsson
authors.forename: H K
authors.initials: HK
authors.lastname: Blomhoff
authors.forename: T
authors.initials: T
authors.lastname: Godal
authors.forename: R
authors.initials: R
authors.lastname: Ohlsson
mesh_terms: D001402:B-Lymphocytes / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D008213:Lymphocyte Activation; D014176:Protein Biosynthesis; D011518:Proto-Oncogene Proteins / Q000032:analysis / Q000235:genetics; D011519:Proto-Oncogenes; D014158:Transcription, Genetic
chemical_list: D011518:Proto-Oncogene Proteins