Differential translational efficiency of the mRNAs isolated from derepressed and glucose repressed Saccharomyces cerevisiae.
Carbon catabolite derepression induced changes in the pool of yeast mRNAs translatable in a protein-synthesizing reticulocyte system. Competition experiments with globin mRNA showed that the mRNA population obtained from derepressed cells possessed a higher translational efficiency than mRNA from repressed cells. The mRNAs that could account for the high translational efficiency of the derepressed mRNA were not detected in cells growing in glucose-rich medium. Analysis of protein synthesis in the presence of 7-methylguanosine 5'-phosphate indicated that the initiation factors recognizing the 5'-terminal structure of capped messengers interacted with lower affinity with the repressed than with some specific derepressed mRNAs.
Soler
A P
AP
Departament de Microbiologia, Facultat de Farmacia, Universitat de València, Spain.
Casanova
M
M
Gozalbo
D
D
Sentandreu
R
R
D004794:Enzyme Repression; D005656:Fungal Proteins / Q000096:biosynthesis; D005947:Glucose / Q000378:metabolism*; D007700:Kinetics; D014176:Protein Biosynthesis; D012331:RNA, Fungal; D012333:RNA, Messenger; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D005656:Fungal Proteins; D012331:RNA, Fungal; D012333:RNA, Messenger; D005947:Glucose