[Radiation-induced instability of yeast chimeric chromosomes].
Instability of the I chimeric chromosome of the yeast Saccharomyces induced by gamma-irradiation has been studied. The chimeric chromosome analysed contained an integrated pYF91 plasmid. Cells of the integrant were irradiated and then mated with non-irradiated cells of the proper tester strain marked by ade1 mutation (red colour of colonies). We isolated 10 hybrids with pink colonies on selective medium. They displayed high degree of mitotic instability during growth on nonselective medium, segregating red colonies (15 to 90% of the total). Tetrad analysis showed that some of the unstable chromosomes exhibited lethal effect in haploids, while others were viable and could pass through meiosis retaining their instability.
Stepanova
V P
VP
Iarovoĭ
B F
BF
Evstiukhina
T A
TA
Zakharov
I A
IA
D002678:Chimera; D005720:Gamma Rays; D009154:Mutation; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000528:radiation effects
Indutsirovannaia oblucheniem nestabil'nost' khimernykh khromosom drozhzheĭ.