A simplified method of production of choline oxidase suitable for choline assay.
A method of production of choline oxidase from Cylindrocarpon-didymum which is of sufficient purity to be used in the enzymatic assaying of choline has been described. The extraction and purification techniques used were ultrasonic disintegration, tangential ultrafiltration, and chromatography on DEAE-Sepharose Fast-Flow and on Sephacryl S 200 gel. The specific activity of the preparation obtained was 7.4 UI/mg of protein. The activity recuperation yield from the cellular extract was 56%. In the course of this purification, a protein displaying catalase activity was also obtained.
Lartillot
S
S
D000429:Alcohol Oxidoreductases / Q000302:isolation & purification*; D002794:Choline / Q000032:analysis*; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D005612:Freeze Drying; D003904:Mitosporic Fungi / Q000201:enzymology*; D013053:Spectrophotometry
D000429:Alcohol Oxidoreductases; C019888:choline oxidase; D002794:Choline