[Preparation of monoclonal antibodies to nuclear DNA of mammalian cells by immunization with group A streptococcal polysaccharide conjugated with synthetic polyelectrolytes].
Monoclonal antibodies (MAb) were obtained by hybridization of spleen cells from BALB/c mice immunized with streptococcal group A polysaccharide (A-PS) conjugated with synthetic polyelectrolytes (PEL). These MAb reacted with nuclei from human and mouse cells. MAb reacting with nuclei were obtained after prolonged immunization with conjugates and were not formed by hybridization of spleen cells from non-immunized mice or by the immunization with PEL. The investigation of Mab (B1/2 and A5/2) reacting with nuclei has shown that these Mab are directed against DNA and do not react with other tissue substances. No cross-reactions of Mab with A-PS used for immunization have been revealed. Mab B1/2 and A5/2 belong to autoantibodies.
Drobyshevskaia
E I
EI
Pyt'eva
E Iu
EIu
Ryzhikova
E V
EV
Borodiuk
N A
NA
Liampert
I M
IM
D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D004247:DNA / Q000276:immunology*; D006801:Humans; D007114:Immunization; D051379:Mice; D011108:Polymers; D011135:Polysaccharides, Bacterial / Q000276:immunology*; D013297:Streptococcus pyogenes / Q000276:immunology*
D000911:Antibodies, Monoclonal; D011108:Polymers; D011135:Polysaccharides, Bacterial; D004247:DNA
Poluchenie monoklonal'nykh antitel k iadernoĭ DNK kletok mlekopitaiushchikh pri immunizatsii polisakharidom streptokokka gruppy A, kon"iugirovannym s sinteticheskimi poliélektrolitami.