Cytotoxicity of a novel lipid-like bacterial product.
A highly cytotoxic, lipid-like compound was isolated from a Serratia marcescens strain currently under identification. We have named the compound DCX for its direct cytotoxic activity on various cell types in culture. DCX was purified by preparative thin layer chromatography from chloroform: methanol = 4:1 extracts of whole bacteria, and is chromatographically homogeneous. The effect of DCX on cells is dose, time, and temperature dependent. DCX is particularly toxic to the mastocytoma cell line P815 (TD50 = 75 pg/ml). Three other malignant or transformed murine cell lines were sensitive to the cytotoxic action of DCX. The effect of DCX was also tested on normal cells (human gingival fibroblasts), which showed greater resistance to DCX than the other cells tested.
Keler
T
T
Thomas W. Evans Museum and Dental Institute, University of Pennyslvania, Philadelphia 19104.
Kovats
E
E
Nguyen
V
V
Samu
J
J
Sanavi
F
F
Somlyo
B
B
Nowotny
A
A
D000818:Animals; D001427:Bacterial Toxins / Q000302:isolation & purification* / Q000494:pharmacology; D002855:Chromatography, Thin Layer; D000089662:Doublecortin Protein; D004351:Drug Resistance; D005347:Fibroblasts / Q000187:drug effects; D006801:Humans; D008055:Lipids / Q000302:isolation & purification* / Q000494:pharmacology / Q000633:toxicity; D051379:Mice; D012706:Serratia marcescens / Q000032:analysis*; D014407:Tumor Cells, Cultured / Q000187:drug effects
D001427:Bacterial Toxins; C000714847:Dcx protein, mouse; D000089662:Doublecortin Protein; D008055:Lipids