A gastrointestinal-specific monoclonal antibody that may be of clinical value in cytologic material.
A new monoclonal antibody (MAb), 29-10, produced by immunization of mice with cells from the SW 1116 colorectal carcinoma cell line, detected an antigen present in cytologic touch imprints of surgically resected normal and neoplastic gastrointestinal (GI) tissue, including specimens from the stomach and the colon. These imprints were fixed in 95% ethanol and stained with the avidin-biotin immunoperoxidase technique. In tested cases, 22 (100%) of 22 imprints from GI adenocarcinomas and from normal GI tissue, as well as 13 (56.6%) of 23 imprints from colonic polyps, stained positively while no staining was demonstrable in imprints from other tissues. In histologic sections, only 4 (23%) of 17 colonic adenocarcinomas and 3 (11.5%) of 26 polyps stained positively. The staining ability of MAb 29-10 was compared to that of MAb 19-9, another colorectal antibody, and was found to be markedly superior for binding of the antigen in cytologic preparations. This tissue-specific antibody may be useful in identifying malignant cells of metastatic carcinoma as to their GI tract origin.
Howell
L P
LP
Department of Pathology, Temple University School of Medicine, Philadelphia, PA 19140.
Zipfel
S
S
Steplewski
Z
Z
Koprowska
I
I
D000230:Adenocarcinoma / Q000473:pathology*; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D003110:Colonic Neoplasms / Q000473:pathology*; D004064:Digestive System / Q000276:immunology*; D006801:Humans; D007124:Immunoenzyme Techniques; D007417:Intestinal Polyps / Q000473:pathology*; D012004:Rectal Neoplasms / Q000473:pathology*
D000911:Antibodies, Monoclonal