Positive effects of indomethacin on restoration of splenic nucleated cell populations in mice given sublethal irradiation.
Splenic cellularity during the recovery phase after 400-R irradiation was evaluated in mice, in which the level of prostaglandin was regulated by indomethacin and exogenous prostaglandin E2, following sublethal irradiation. Two weeks after irradiation, administration to these mice of indomethacin, an inhibitor of prostaglandin synthesis, augmented the recovery of all nucleated spleen cell populations, whereas the thymus was drastically depopulated. This treatment had little effect upon the total number of bone marrow cells but inversed the ratio of PNA+/PNA- cells. Cell transfer experiments using heavily irradiated mice as recipients showed that the stem cell proliferation was positively affected by indomethacin treatment in the bone marrow rather than in the spleen. These results suggest that cell migration from primary lymphoid organs, particularly from the bone marrow to the spleen, is regulated by a prostaglandin-mediated system and that a prostaglandin E2 synthesis inhibition would have a positive effect on the restoration of peripheral nucleated cells following irradiation.
Serushago
B A
BA
Department of Immunology, Kyushu University, Fukuoka, Japan.
Tanaka
K
K
Koga
Y
Y
Taniguchi
K
K
Nomoto
K
K
D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D001853:Bone Marrow / Q000187:drug effects / Q000528:radiation effects; D006412:Hematopoietic Stem Cells / Q000187:drug effects* / Q000528:radiation effects; D007213:Indomethacin / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008806:Mice, Inbred AKR; D008809:Mice, Inbred C3H; D011453:Prostaglandins / Q000097:blood; D011975:Receptors, Mitogen / Q000032:analysis; D013154:Spleen / Q000187:drug effects* / Q000528:radiation effects; D013950:Thymus Gland / Q000187:drug effects / Q000528:radiation effects; D014916:Whole-Body Irradiation
D000954:Antigens, Surface; D011453:Prostaglandins; D011975:Receptors, Mitogen; C038223:peanut agglutinin receptor; D007213:Indomethacin