Purification of KBF1, a common factor binding to both H-2 and beta 2-microglobulin enhancers.
An enhancer binding factor, designated KBF1, has been purified from the nuclear extract of mouse BW5147 thymoma cells by five column chromatography steps including a sequence-specific DNA affinity column. Gel retardation and footprint analysis have shown that purified KBF1 has a binding activity specific for both H-2 and beta 2-microglobulin enhancer sequences. After SDS-polyacrylamide gel electrophoresis of the most purified preparation a 48-kd protein showed, after elution and renaturation, a binding activity to both enhancer sequences. These findings suggest that the expression of both H-2 and beta 2-microglobulin genes utilizes a common regulatory mechanism.
Yano
O
O
Unité de Biologie Moléculaire du Gène, Institut National de la Santé et de la Recherche Médicale, Institut Pasteur, Paris, France.
Kanellopoulos
J
J
Kieran
M
M
Le Bail
O
O
Israël
A
A
Kourilsky
P
P
D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D004742:Enhancer Elements, Genetic; D005809:Genes, Regulator; D006183:H-2 Antigens / Q000235:genetics*; D051379:Mice; D008970:Molecular Weight; D013945:Thymoma; D013953:Thymus Neoplasms; D001613:beta 2-Microglobulin / Q000235:genetics*
D002352:Carrier Proteins; D006183:H-2 Antigens; D001613:beta 2-Microglobulin