title: Primary structures of M6 and M7 of mugiline beta (Mugil japonicus).
abstract: Mugiline beta isolated from mature sperm nuclei of the Formosan grey mullet, belonging to Perciformes, was fractionated into seven components (M1-M7), by chromatography on CM-Sephadex C-25. The amino acid sequences of the two major components (M6 and M7) were then determined. M6 contained 33 amino acid residues per molecule: Arg, 21; Thr, 1; Ser, 1; Glu, 1; Pro, 3; Ala, 2; Val, 2; Met, 0.3 and Ile, 1.7. The amino acid sequence of M6 is: Pro-Arg-Arg-Arg-Arg-Glu-Thr-Ser-Arg-Pro-Ile-Arg-Arg-Arg-Arg-Arg-Ala-Pro- Ile (Met)-Arg-Arg-Arg-Arg-Arg-Val-Val-Arg-Arg-Arg-Arg. Isoleucine at position 22 is partially replaced by methionine. M7 had an amino acid sequence similar to that of M6 except that glutamic acid at position 6 of M6 was replaced by glutamine. A high degree of homology in the sequences was found between mugiline beta from mullet and thynnine from tuna fish, which also belongs to Perciformes.
authors.forename: Y
authors.initials: Y
authors.lastname: Okamoto
authors.forename: E
authors.initials: E
authors.lastname: Muta
authors.forename: S
authors.initials: S
authors.lastname: Ota
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001331:Automation; D002845:Chromatography; D003488:Cyanogen Bromide; D010450:Endopeptidases; D005399:Fishes / Q000502:physiology*; D008297:Male; D011479:Protamines / Q000032:analysis*; D012697:Serine Endopeptidases; D013094:Spermatozoa / Q000032:analysis
chemical_list: D000596:Amino Acids; D011479:Protamines; C053431:mugiline beta; D010450:Endopeptidases; D012697:Serine Endopeptidases; C028344:glutamyl endopeptidase; D003488:Cyanogen Bromide