title: Detection of antibodies to human immunodeficiency virus by latex agglutination with recombinant antigen.
abstract: Recombinant human immunodeficiency virus (HIV) env antigen was attached to polystyrene particles, and these complexes were used to develop the first latex agglutination assay for antibodies to HIV. A total of 95 positive and 116 negative human serum samples were assayed for antibodies to HIV by latex agglutination, and results were compared with those of a commercial enzyme immunoassay. Latex agglutination was also compared with, and found to be completely concordant with, Western blot (immunoblot) analysis with virion antigens.
authors.affiliation: Cambridge BioScience Corporation, Worcester, Massachusetts 01605.
authors.forename: C H
authors.initials: CH
authors.lastname: Riggin
authors.forename: G A
authors.initials: GA
authors.lastname: Beltz
authors.forename: C H
authors.initials: CH
authors.lastname: Hung
authors.forename: R M
authors.initials: RM
authors.lastname: Thorn
authors.forename: D J
authors.initials: DJ
authors.lastname: Marciani
mesh_terms: D000914:Antibodies, Viral / Q000032:analysis*; D000956:Antigens, Viral / Q000276:immunology*; D006678:HIV / Q000276:immunology*; D015483:HIV Antibodies; D015488:HIV Antigens; D006801:Humans; D007118:Immunoassay; D007124:Immunoenzyme Techniques; D007841:Latex Fixation Tests; D011237:Predictive Value of Tests; D011994:Recombinant Proteins / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D015483:HIV Antibodies; D015488:HIV Antigens; D011994:Recombinant Proteins