title: Developmental approaches to the analysis of vertebrate central pattern generators.
abstract: The isolated spinal cord of the chick embryo is a new preparation for analyzing the neural mechanisms and development of vertebrate motor activity. The embryonic cord can be isolated in vitro during the period of development when antagonist alternation of hindlimb motoneurons matures. The preparation is spontaneously active in vitro generating episodes of motor activity that can be recorded from muscle nerves and the ventral roots. The neural mechanisms responsible for the development and genesis of motor activity are being investigated using intra- and extracellular recording from motoneurons and electrotonic recordings of motoneuron synaptic activity from muscle nerves. The results suggest that alternating motor activity in the isolated chick cord may be generated by a mechanism in which a synaptically induced motoneuronal shunt conductance regulates the time of discharge of flexor and extensor motoneurons.
authors.affiliation: Department of Physiology and Biophysics, University of Iowa, Iowa City 52242.
authors.forename: M J
authors.initials: MJ
authors.lastname: O'Donovan
mesh_terms: D000200:Action Potentials; D000818:Animals; D002642:Chick Embryo; D066298:In Vitro Techniques; D008564:Membrane Potentials; D009046:Motor Neurons / Q000196:embryology / Q000502:physiology*; D009068:Movement; D013116:Spinal Cord / Q000196:embryology / Q000502:physiology*; D013126:Spinal Nerve Roots / Q000502:physiology