c-myc amplification and expression in newly established human osteosarcoma cell lines.
Three cell lines were isolated from a patient with osteosarcoma of the femur. These lines were obtained from the primary neoplasm before (HTLA145) and after (HTLA161) chemotherapy and from a metastasis of the lung (HTLA195) in the same patient. The three cell lines exhibited a similar morphology in culture and formed tumors in nude mice which demonstrated a histopathology similar to that which had been observed in the patient. High expression of the genes coding for the alpha-1 and alpha-2 chain of collagen Type I was found in vitro and in s.c. tumors growing in nude mice. The c-myc protooncogene was amplified in all three cell lines and extensive expression of c-myc was found in vitro and in vivo. No heterogeneity in regard to c-myc expression in vivo was detected by in situ localization in tumors growing in nude mice.
Bogenmann
E
E
Moghadam
H
H
DeClerck
Y A
YA
Mock
A
A
D000818:Animals; D001859:Bone Neoplasms / Q000235:genetics*; D002460:Cell Line; D003094:Collagen / Q000235:genetics; D005784:Gene Amplification; D005786:Gene Expression Regulation; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D008858:Microscopy, Phase-Contrast; D009368:Neoplasm Transplantation; D009693:Nucleic Acid Hybridization; D009857:Oncogenes; D012516:Osteosarcoma / Q000235:genetics*; D014158:Transcription, Genetic; D014183:Transplantation, Heterologous
D003094:Collagen