Action of chlorhexidine on budding Candida albicans: scanning and transmission electron microscopic study.
Chlorhexidine is widely used as a bacterial drug whose method of action has been well described in bacteria. Its fungicidal properties have been proved. We show here the effects of a sublethal dose of a preparation of digluconate of chlorhexidine on budding Candida albicans. A fungistatic action is revealed by a decrease in the percentage of budding cells, and two main types of alterations can be observed with transmission electron microscopy (T.E.M.): a loss of cytoplasmic components and a coagulation of nucleoproteins. With scanning electron microscopy (S.E.M.), the cell walls show morphological modifications.
Bobichon
H
H
Laboratoire de Biologie Végétale et Cryptogamie, Faculté de Pharmacie, Reims, France.
Bouchet
P
P
D002176:Candida albicans / Q000187:drug effects* / Q000254:growth & development / Q000648:ultrastructure; D002710:Chlorhexidine / Q000494:pharmacology*; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning
D002710:Chlorhexidine