Ectophospholipase A2 activity of the rabbit peritoneal neutrophil.
Intact rabbit neutrophils were found to express phospholipase A2 activity against [14C]oleate-labeled autoclaved Escherichia coli. Cells obtained 12-14 h rather than 4 h after intraperitoneal injection of glycogen had approximately threefold higher activity. The higher activity was due neither to contaminating mono-nuclear cells nor to the degranulation associated with the prolonged inflammatory response in the peritoneum. Of the phospholipase A2 activity of the intact 12-hour cells, approximately one half remained cell-associated, but the other half was released from the neutrophils during incubation. The cell-associated activity was not due to phagocytosis of substrate and subsequent release of [14C]fatty acid. The cell-associated activity was inhibited by the membrane-impermeant diazonium salt of sulfanilic acid. The results indicate that the cell-associated activity of intact neutrophils is due to an ectophospholipase A2.
Kennedy
S P
SP
Becker
E L
EL
D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D002499:Centrifugation, Density Gradient; D003979:Diazonium Compounds / Q000494:pharmacology; D004926:Escherichia coli / Q000276:immunology; D009504:Neutrophils / Q000201:enzymology*; D010529:Peritoneal Cavity / Q000166:cytology; D010587:Phagocytosis; D010740:Phospholipases / Q000378:metabolism*; D010741:Phospholipases A / Q000032:analysis / Q000378:metabolism*; D054467:Phospholipases A2; D011817:Rabbits / Q000276:immunology*
D003979:Diazonium Compounds; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2