Influence of cartilage on reactivity and on the effectiveness of verapamil in guinea pig isolated airway smooth muscle.
The authors have examined the effects of cartilage removal on smooth muscle reactivity and the action of verapamil in guinea pig trachealis. In preparations devoid of cartilage, smooth muscle reactivity to both histamine and KCl was reduced. Reactivity to methacholine was unaffected by cartilage removal. In the absence of cartilage, verapamil had a greater depressant effect on the maximum responses to histamine and methacholine than in intact tissues. Similarly, verapamil was more potent against histamine- and methacholine-induced responses in the absence of cartilage where a greater shift to the right was seen in the concentration-response curves when compared with cartilage-containing controls. The spasmolytic action of verapamil on methacholine-induced responses was greater in the absence of cartilage and was greater than its antispasmogenic activity against methacholine (whether or not cartilage was present). Thus, cartilage removal reduces muscle reactivity and increases the potency of verapamil in guinea pig trachealis.
Raeburn
D
D
Hay
D W
DW
Farmer
S G
SG
Fedan
J S
JS
D000818:Animals; D001993:Bronchodilator Agents / Q000494:pharmacology; D002356:Cartilage / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D006168:Guinea Pigs; D006632:Histamine / Q000494:pharmacology; D066298:In Vitro Techniques; D008297:Male; D016210:Methacholine Chloride; D008688:Methacholine Compounds / Q000494:pharmacology; D009130:Muscle, Smooth / Q000187:drug effects* / Q000502:physiology; D009994:Osmolar Concentration; D011189:Potassium Chloride / Q000494:pharmacology; D014132:Trachea / Q000187:drug effects* / Q000502:physiology; D014700:Verapamil / Q000494:pharmacology*
D001993:Bronchodilator Agents; D008688:Methacholine Compounds; D016210:Methacholine Chloride; D011189:Potassium Chloride; D006632:Histamine; D014700:Verapamil