Electrophoretic separation of cells.
There are several kinds of cell electrophoresis. The most important are free-flow electrophoresis, scaled-up free-flow electrophoresis and column electrophoresis. All kinds of cell electrophoresis, especially free-flow electrophoresis, have been improved to a very high standard of separation accuracy, and their application possibilities are extended when antigen-specific electrophoretic cell separation is performed, or when cell electrophoresis is combined with other physical methods for cell separation. Cell electrophoresis and other physical cell separation methods have the advantage that the functional state of the cells remains virtually unchanged during the isolation procedure. With the help of cell electrophoresis monocytes, T-lymphocytes, platelets and other cells from human peripheral blood could be purified. Other human cells were enriched for immunological characterization. Furthermore, cells that secreted human plasminogen activators or human antibodies were electrophoresed to give cell fractions with increased frequencies of the cells of interest.
Bauer
J
J
D000818:Animals; D003584:Cytological Techniques / Q000295:instrumentation*; D004586:Electrophoresis; D006801:Humans