Effect of GDP on the interactions between chloroplast EF-Ts and chloroplast and E. coli EF-Tu.
The effects of varying concentrations of GDP on the stability of homologous and heterologous EF-Tu:EF-Ts complexes formed with the elongation factors from the chloroplast of Euglena gracilis and from E. coli have been investigated. The complexes formed with chloroplast EF-Ts were significantly more stable to GDP-induced dissociation than those formed with E. coli EF-Ts. The complex between chloroplast EF-Tu and chloroplast EF-Ts required nearly 1,000-fold higher concentrations of GDP for dissociation than the complex between chloroplast EF-Tu and E. coli EF-Ts. The E. coli EF-Tu:chloroplast EF-Ts complex required nearly 100-fold higher levels of GDP for dissociation than the E. coli EF-Tu:E. coli EF-Ts complex.
Spremulli
G H
GH
Department of Chemistry, University of North Carolina, Chapel Hill 27514.
Spremulli
L L
LL
D002736:Chloroplasts / Q000502:physiology*; D004926:Escherichia coli / Q000502:physiology; D006150:Guanine Nucleotides / Q000494:pharmacology*; D006153:Guanosine Diphosphate / Q000494:pharmacology*; D046911:Macromolecular Substances; D010444:Peptide Elongation Factor Tu / Q000502:physiology*; D010445:Peptide Elongation Factors / Q000502:physiology*; D013045:Species Specificity
D006150:Guanine Nucleotides; D046911:Macromolecular Substances; D010445:Peptide Elongation Factors; C023512:elongation factor Ts; D006153:Guanosine Diphosphate; D010444:Peptide Elongation Factor Tu