[Adsorption of antigens K88 and K99 on aluminum hydroxide gel].
Studied was the effect of the pH value of the medium, of the concentration of the antigen and the adsorbent on the adsorption of K 88 and K 99 on aluminium hydroxide-gel. Use was made of strains O 45:K 88 and O 14:K 99. The optimal parameters of pH and the concentration of aluminium oxide as well as of aluminium hydroxide-gel as an adjuvant, substantiating the maximum adsorption capacity of the latter with regard to the two Escherichia coli antigens used. Established was the maximum amount of the antigens which could be adsorbed by as much as 1 cm3 of aluminium hydroxide-gel. According to the authors such amount could serve to indicate the way how to produce effective preparations to be deposited with the two-antigen adjuvant.
Kapurdova
M
M
Petkov
M
M
Marchev
N
N
D000327:Adsorption; D000536:Aluminum Hydroxide; D000942:Antigens, Bacterial; D000954:Antigens, Surface / Q000032:analysis*; D001427:Bacterial Toxins; D004305:Dose-Response Relationship, Drug; D004306:Dose-Response Relationship, Immunologic; D004926:Escherichia coli / Q000276:immunology*; D029968:Escherichia coli Proteins; D036781:Fimbriae Proteins; D006863:Hydrogen-Ion Concentration; D007163:Immunosorbent Techniques
D000942:Antigens, Bacterial; D000954:Antigens, Surface; D001427:Bacterial Toxins; D029968:Escherichia coli Proteins; C026888:K88 antigen, E coli; C031429:K99 antigen; D036781:Fimbriae Proteins; D000536:Aluminum Hydroxide
Adsorbtsiia na antigeni K 88 i K 99 vŭrkhu aluminiev khidrookis-gel.