title: Role of phenylalanine 150 in the receptor-binding domain of the K88 fibrillar subunit.
abstract: Recently, we reported the isolation of three peptides, Ile-83-Ala-Phe-85, Ser-148-Leu-Phe-150, and Ala-156-Ile-Phe-158, derived from the K88 fibrillar subunit and found to inhibit the binding of K88 fibrillae to cavia erythrocytes or pig intestinal epithelial cells (A. A. C. Jacobs, J. Venema, R. Leeven, H. van Pelt-Heerschap, and F. K. de Graaf, J. Bacteriol. 169:735-741, 1987). The gene encoding the K88 fibrillar adhesin was modified by oligonucleotide-directed site-specific mutagenesis such that each of the phenylalanine residues at positions 85, 150, and 158 were replaced by serine. Replacement of phenylalanine 85 or 158 had no apparent effect on the biosynthesis of the fibrillae or on their adhesive capacity. In contrast, substitution of phenylalanine 150 with serine resulted in a dramatic decrease in adhesive capacity of the K88 fibrillae. Apparently, phenylalanine 150 plays an essential role in the interaction of the adhesin with receptor molecules present on eucaryotic cells.
authors.affiliation: Department of Microbiology, Vrije Universiteit, Amsterdam, The Netherlands.
authors.forename: A A
authors.initials: AA
authors.lastname: Jacobs
authors.forename: B
authors.initials: B
authors.lastname: Roosendaal
authors.forename: J F
authors.initials: JF
authors.lastname: van Breemen
authors.forename: F K
authors.initials: FK
authors.lastname: de Graaf
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial; D000954:Antigens, Surface / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D001422:Bacterial Adhesion; D001665:Binding Sites; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D029968:Escherichia coli Proteins; D036781:Fimbriae Proteins; D006384:Hemagglutination; D008970:Molecular Weight; D009154:Mutation; D010649:Phenylalanine; D010957:Plasmids; D011485:Protein Binding
chemical_list: D000942:Antigens, Bacterial; D000954:Antigens, Surface; D029968:Escherichia coli Proteins; C026888:K88 antigen, E coli; D036781:Fimbriae Proteins; D010649:Phenylalanine