title: [The demonstration of proteolytic activity of bovine coronary arteries and the effect of drugs and other substances on it].
abstract: Intracellular neutral proteinases activity of bovine coronary arteries was demonstrated, isolated and partially characterized. The enriched crude enzyme preparation can be inhibited by diisopropylphosphofluoridate (DFP) and phenylmethylsulfonylfluoride (PMSF). The inhibitor concentration (pI50 values) by DFP and PMSF in the enzyme preparation and for trypsin are very similar. Several drugs (isoproterenol, propranolol, theophylline) tested on the enriched crude neutral proteinases preparation showed stimulatory or inhibitory activities. The results are discussed with regard to a possible action mechanism such proteinases on the cyclic AMP/cyclic GMP system.
authors.affiliation: Ernst-Moritz-Arndt-Universität Greifswald, Institut für Pharmakologie und Toxikologie.
authors.forename: K U
authors.initials: KU
authors.lastname: Möritz
authors.forename: A
authors.initials: A
authors.lastname: Grisk
authors.forename: A
authors.initials: A
authors.lastname: Klassen
mesh_terms: D000818:Animals; D002417:Cattle; D003331:Coronary Vessels / Q000201:enzymology*; D066298:In Vitro Techniques; D008970:Molecular Weight; D009131:Muscle, Smooth, Vascular / Q000201:enzymology*; D010447:Peptide Hydrolases / Q000302:isolation & purification / Q000378:metabolism*; D011480:Protease Inhibitors / Q000494:pharmacology; D013347:Subcellular Fractions / Q000201:enzymology
chemical_list: D011480:Protease Inhibitors; D010447:Peptide Hydrolases
vernacular_title: Zum Nachweis von proteolytischer Aktivität in Rindercoronararterien und deren Beeinflussbarkeit durch Pharmaka und andere Stoffe.