Expression of leucine genes from an extremely thermophilic bacterium in Escherichia coli.
The organisation of the leucine genes in Thermus thermophilus HB8 was analysed by examining the ability of recombinant DNAs to complement Escherichia coli mutations. The arrangement of the genes is different from that in the mesophilic bacteria E. coli and Salmonella typhimurium. The promoter responsible for the expression of the leuB, leuC and leuD genes of Thermus HB8 in E. coli was identified. The sequence of Thermus DNA containing this promoter revealed structural similarities to the promoter and attenuator regions of the E. coli leucine operon.
Croft
J E
JE
Cell Biology Department, University of Auckland, New Zealand.
Love
D R
DR
Bergquist
P L
PL
D050539:3-Isopropylmalate Dehydrogenase; D000429:Alcohol Oxidoreductases / Q000235:genetics*; D001483:Base Sequence; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D006836:Hydro-Lyases / Q000235:genetics*; D007930:Leucine / Q000235:genetics*; D008969:Molecular Sequence Data; D009876:Operon; D011401:Promoter Regions, Genetic; D013824:Thermus / Q000235:genetics*
D000429:Alcohol Oxidoreductases; D050539:3-Isopropylmalate Dehydrogenase; D006836:Hydro-Lyases; C021455:3-isopropylmalate dehydratase; D007930:Leucine