title: Transformation of Aspergillus nidulans with the hygromycin-resistance gene, hph.
abstract: Aspergillus nidulans strain G191 was transformed to hygromycin resistance using plasmid pDH25, which contains the bacterial hygromycin B phosphotransferase gene (hph) fused to promoter elements of the A. nidulans trpC gene. Southern hybridizations of transformants revealed multiple, integrated copies of the vector. A pleiotropic effect conferring increased hygromycin B sensitivity was found to be associated with the A. nidulans pyrG89 allele. Plasmid pDH25 features a ClaI site immediately preceding the hph start codon thus permitting convenient replacement of the trpC sequences with other eukaryotic promoters.
authors.affiliation: Forest Products Laboratory, USDA, Madison, WI 53705.
authors.forename: D
authors.initials: D
authors.lastname: Cullen
authors.forename: S A
authors.initials: SA
authors.lastname: Leong
authors.forename: L J
authors.initials: LJ
authors.lastname: Wilson
authors.forename: D J
authors.initials: DJ
authors.lastname: Henner
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001233:Aspergillus nidulans / Q000187:drug effects / Q000235:genetics*; D001483:Base Sequence; D004352:Drug Resistance, Microbial; D005800:Genes, Fungal; D005838:Genotype; D006921:Hygromycin B / Q000494:pharmacology*; D010957:Plasmids; D014170:Transformation, Genetic
chemical_list: D000900:Anti-Bacterial Agents; D006921:Hygromycin B