[Detection and quantification of erythrocyte-associated immunoglobulins using immunoenzyme technic--enzyme immunometric assay (EIMA)].
A new technique is described for the quantification of erythrocyte-associated immunoglobulin G (EAIgG). Enzyme immunometric assay (EIMA) measures the consumption of enzyme-labelled anti-human globulin. Thus, the release of cellular enzymes is avoided and, thereby also falsification of the results owing to the antigen-antibody reaction taking place in media of differing molarity and ionic strength (as is inevitable in red cell lysis assays). This consumption technique is more sensitive than simple "sandwich" procedures. The measurement of minimal amounts of physiologically bound EAIgG is, moreover, possible.
Winter
P M
PM
Institut für Blutgruppenserologie, Universität Wien.
Müller-Uri
P
P
D000744:Anemia, Hemolytic, Autoimmune / Q000276:immunology; D004899:Erythroblastosis, Fetal / Q000276:immunology; D004912:Erythrocytes / Q000276:immunology*; D006801:Humans; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000032:analysis*; D007231:Infant, Newborn
D007074:Immunoglobulin G
Nachweis und Quantifizierung von erythrozyten-assoziierten Immunoglobulinen mittels Enzymimmuntechnik--Enzyme Immunometric Assay (EIMA).