Improvement in the antibody binding characteristics of microtitre wells by pretreatment with anti-IgG Fc immunoglobulin.
A preliminary coating of the surface of polystyrene microtitre wells with anti-IgG Fc immunoglobulin improves the binding capacity of the wells for antibody and leads to a marked improvement in the reproducibility of immunoassays carried out in the wells. In an enhanced chemiluminescent enzyme immunoassay for oestradiol the binding capacity for oestradiol of wells precoated with anti-IgG Fc immunoglobulin and then coated with anti-oestradiol immunoglobulin was more than twice that of wells coated directly with anti-oestradiol immunoglobulin. Intra-assay precision in the precoated wells ranged from 1.3 to 4.0% compared to 2.9 to 6.7% (0-3200 fmol oestradiol) in wells which had not been precoated.
Sankolli
G M
GM
Department of Clinical Chemistry, Wolfson Research Laboratories, Queen Elizabeth Medical Centre, Birmingham, U.K.
Stott
R A
RA
Kricka
L J
LJ
D000888:Antibodies, Anti-Idiotypic / Q000276:immunology; D004306:Dose-Response Relationship, Immunologic; D004958:Estradiol / Q000032:analysis* / Q000276:immunology; D007141:Immunoglobulin Fc Fragments / Q000276:immunology; D007163:Immunosorbent Techniques; D008163:Luminescent Measurements; D010969:Plastics
D000888:Antibodies, Anti-Idiotypic; D007141:Immunoglobulin Fc Fragments; D010969:Plastics; D004958:Estradiol