Purification of the lipopolysaccharide fraction from Klebsiella pneumoniae O1 K2 by high-performance liquid chromatography.
An high-performance liquid chromatography technique was applied to purify the lipopolysaccharide fraction from a lysate of Klebsiella pneumoniae O1 K2 (NCTC 5055). The separation of the lipopolysaccharide fraction from the proteins was carried out with a reversed-phase column. By this method the lipopolysaccharide fraction was obtained in a pure state, devoid of proteins but possessing the same biological properties as the lipopolysaccharide fraction prepared by the classical phenol-water technique.
Kol
O
O
Montreuil
J
J
Fournet
B
B
Zalisz
R
R
Smets
P
P
D001426:Bacterial Proteins / Q000032:analysis; D002241:Carbohydrates / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D005227:Fatty Acids / Q000032:analysis; D007711:Klebsiella pneumoniae / Q000032:analysis*; D008070:Lipopolysaccharides / Q000302:isolation & purification*; D013056:Spectrophotometry, Ultraviolet
D001426:Bacterial Proteins; D002241:Carbohydrates; D005227:Fatty Acids; D008070:Lipopolysaccharides