Rapid in vivo assay method for type G botulinal toxin.
The survival time (ST) dose-response curve for Clostridium botulinum type G toxin was determined in mice and evaluated as a rapid assay method. As it occurs with other botulinal toxin types, the results showed a linear relationship between the logarithm of the injected dose and the logarithm of ST. The slope of the ST dose-response curve for type G toxin differed significantly from those for type A or subtype Af toxins. This parameter was altered when trypsin-activated type G toxin was used. The ST dose-response curve was rather stable. This in vivo assay method could be applied for the estimation of the potency of G toxin preparations in a short time using only some few mice.
Mayorga
L S
LS
de Centorbi
O P
OP
de Milan
C C
CC
Gimenez
D F
DF
D000818:Animals; D001681:Biological Assay; D001905:Botulinum Toxins / Q000032:analysis* / Q000633:toxicity; D003014:Clostridium botulinum / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D051379:Mice; D012044:Regression Analysis
D001905:Botulinum Toxins