Sedimentation characteristics of vesicles associated with insulin-sensitive intracellular glucose transporter from rat adipocytes.
The sedimentation characteristics of vesicles associated with the insulin-sensitive intracellular glucose transporter from rat adipocytes were studied. The method used was sucrose density gradient centrifugation, which was carried out under non-equilibrium and equilibrium (isopycnic) conditions. The glucose transport activity was determined by the reconstitution method. As reported previously, the sedimentation velocity of the intracellular glucose-transport activity was considerably slower than that of the counterpart in the plasma membrane. It was found, however, that the specific gravity of the slow-sedimenting glucose-transport activity was almost identical to that of the activity in the plasma membrane (d = 1.118-1.122). It is concluded that the intracellular glucose transport activity is associated not with low-density microsomal vesicles, but with unidentified slow-sedimenting vesicles that have a specific gravity similar to that of the plasma membrane.
Shibata
Y
Y
Flanagan
J E
JE
Smith
M M
MM
Robinson
F W
FW
Kono
T
T
D000273:Adipose Tissue / Q000648:ultrastructure*; D000818:Animals; D002458:Cell Fractionation; D002462:Cell Membrane / Q000378:metabolism; D002500:Centrifugation, Isopycnic; D007328:Insulin / Q000494:pharmacology*; D007425:Intracellular Membranes / Q000378:metabolism*; D008297:Male; D009004:Monosaccharide Transport Proteins / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D007328:Insulin; D009004:Monosaccharide Transport Proteins