Spontaneous production of interleukin 1 activity by chronic lymphocytic leukemic cells.
Chronic lymphocytic leukemic B cells (B-CLL) were found to produce an IL 1-like growth factor spontaneously in vitro for mouse thymocytes. This factor was comitogenic with concanavalin A (Con A) and nonmitogenic combinations of phorbol ester and calcium ionophore but not with phyto-hemagglutinin (PHA). Growth factor production was dose-related to the number of in vitro cultured cells and detectable at 6 hours using high cell concentrations. A small number of admixed normal T cells was not important for factor production. No growth of autologous B-CLL or allogeneic thymocytes was induced by the factor. A chromatographic high-performance liquid chromatography (HPLC) analysis and inhibition experiments with a polyclonal rabbit anti interleukin 1 (IL 1) antiserum indicated that the B-CLL-derived growth factor belonged to the IL 1 family. This was supported by the direct demonstration of IL 1 beta in supernatants from B-CLL by radioimmunoassay. Possible biologic implications for B-CLL-derived IL 1 are discussed in relation to tumor cell growth in different clinical stages.
Uggla
C
C
Department of Immunology, Karolinska Institute, Stockholm, Sweden.
Aguilar-Santelises
M
M
Rosén
A
A
Mellstedt
H
H
Jondal
M
M
D001402:B-Lymphocytes / Q000502:physiology*; D006133:Growth Substances / Q000096:biosynthesis*; D007158:Immunologic Techniques; D007375:Interleukin-1 / Q000096:biosynthesis* / Q000276:immunology; D007376:Interleukin-2 / Q000096:biosynthesis; D015847:Interleukin-4; D007378:Interleukins / Q000096:biosynthesis; D007945:Leukemia, Lymphoid / Q000503:physiopathology*; D008213:Lymphocyte Activation; D011863:Radioimmunoassay; D014407:Tumor Cells, Cultured / Q000502:physiology*
D006133:Growth Substances; D007375:Interleukin-1; D007376:Interleukin-2; D007378:Interleukins; D015847:Interleukin-4