title: Control of human thyroid follicular cell proliferation in suspension and monolayer culture.
abstract: We have used both suspension and monolayer cultures to investigate the in vitro control of normal human thyroid follicular cell proliferation as assessed by both [3H]TdR incorporation and autoradiography. In suspension cultures, whilst TSH alone produced no effect, inclusion of two permissive factors, namely a supraphysiological concentration of insulin (80 micrograms/ml) and a low concentration of FCS (1%) (which, in the absence of TSH, gave only a weak proliferative response) permitted a highly significant response to TSH at 0.1 mU/ml. A higher concentration of TSH (10 mU/ml) resulted in a reduced response. In monolayer cultures in contrast, insulin and FCS were themselves highly growth stimulatory, with no super-added effect on addition of TSH. Our data indicate that suspension culture provides a more valid in vitro model for the investigation of growth factor control of normal and neoplastic human thyroid epithelium.
authors.forename: D W
authors.initials: DW
authors.lastname: Williams
authors.forename: D
authors.initials: D
authors.lastname: Wynford-Thomas
authors.forename: E D
authors.initials: ED
authors.lastname: Williams
mesh_terms: D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003470:Culture Media; D004247:DNA / Q000096:biosynthesis; D004847:Epithelial Cells; D006133:Growth Substances / Q000494:pharmacology; D006801:Humans; D007328:Insulin / Q000494:pharmacology; D013961:Thyroid Gland / Q000166:cytology*; D013972:Thyrotropin / Q000494:pharmacology
chemical_list: D003470:Culture Media; D006133:Growth Substances; D007328:Insulin; D013972:Thyrotropin; D004247:DNA