[Sorption properties of polystyrene plates used in immunoenzyme analyses].
The capacity of polystyrene carriers used in the enzyme immunoassay (EIA) for adsorbing 131I-labeled human serum albumin under different conditions has been studied, and the comparison of the plates manufactured by Dynatech AG (Switzerland) and by the Leningrad Works of Medical Polymers has been made. At the first stages of the reaction the antigen is separated from the carrier and the amount of the desorbed antigen depends on its initial dose and the dilution of the assayed sera. The irregular desorption of the antigen leads to misinterpretation of the results. Comparison of the polystyrene plates has shown that each plate is characterized by individual adsorption capacity, which impedes at present the standardization of EIA-based test systems.
Sukhorukov
A M
AM
Ponomareva
A M
AM
D000327:Adsorption; D000941:Antigens / Q000032:analysis; D005069:Evaluation Studies as Topic; D006801:Humans; D007124:Immunoenzyme Techniques / Q000295:instrumentation* / Q000592:standards; D011137:Polystyrenes; D012711:Serum Albumin, Radio-Iodinated / Q000276:immunology
D000941:Antigens; D011137:Polystyrenes; D012711:Serum Albumin, Radio-Iodinated
Izuchenie sorbtsionnykh svoĭstv polistirolovykh planshetov, ispol'zuemykh v immunofermentnom analize.