Backbone dynamics of a model membrane protein: assignment of the carbonyl carbon 13C NMR resonances in detergent-solubilized M13 coat protein.
The major coat protein of the filamentous bacteriophage M13 is a 50-residue amphiphilic polypeptide which is inserted, as an integral membrane-spanning protein, in the inner membrane of the Escherichia coli host during infection. 13C was incorporated biosynthetically into a total of 23 of the peptide carbonyls using labeled amino acids (alanine, glycine, lysine, phenylalanine, and proline). The structure and dynamics of carbonyl-labeled M13 coat protein were monitored by 13C nuclear magnetic resonance (NMR) spectroscopy. Assignment of many resonances was achieved by using protease digestion, pH titration, or labeling of the peptide bond with both 13C and 15N. The carbonyl region of the natural-abundance 13C NMR spectrum of M13 coat protein in sodium dodecyl sulfate solution shows approximately eight backbone carbonyl resonances with line widths much narrower than the rest. Three of these more mobile residues correspond to assigned peaks (glycine-3, lysine-48, and alanine-49) in the individual amino acid spectra, and another almost certainly arises from glutamic acid-2. A ninth residue, alanine-1, also gives rise to a very narrow carbonyl resonance if the pH is well above or below the pKa of the terminal amino group. These data suggest that only about four residues at either end of the protein experience large-amplitude spatial fluctuations; the rest of the molecule is essentially rigid on the time scale of the overall rotational tumbling of the protein-detergent complex. The relative exposure of different regions of detergent-bound protein was monitored by limited digestion with proteinase K.(ABSTRACT TRUNCATED AT 250 WORDS)
Henry
G D
GD
Medical Research Council Group in Protein Structure and Function, University of Alberta, Edmonton, Canada.
Weiner
J H
JH
Sykes
B D
BD
D000595:Amino Acid Sequence; D002213:Capsid; D036022:Capsid Proteins; D002250:Carbon Radioisotopes; D003090:Coliphages / Q000378:metabolism; D003840:Deoxycholic Acid; D004926:Escherichia coli / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008565:Membrane Proteins; D011487:Protein Conformation; D012967:Sodium Dodecyl Sulfate; D012995:Solubility
D036022:Capsid Proteins; D002250:Carbon Radioisotopes; D008565:Membrane Proteins; C025668:coat protein, Bacteriophage M13; D003840:Deoxycholic Acid; D012967:Sodium Dodecyl Sulfate