Development of a practicable method for isolation and identification of dengue viruses in developing countries.
We sought a practicable method for isolation and identification of dengue viruses in South-East Asia. We compared two mosquito cell lines, C6/36 and TRA-284-SFG, for virus isolation and two identification methods, immunofluorescent staining of infected cells with serotype-specific mouse monoclonal antibodies and a sandwich-type enzyme-linked immunosorbent assay with conventional mouse hyperimmune ascitic fluids. We found that the combination of TRA-284-SFG cells and ELISA is a useful and feasible method in developing countries.
Nawa
M
M
Department of Microbiology, Saitama Medical School, Japan.
Torres
C A
CA
Paladin
F J
FJ
Tupasi
T E
TE
Kaneko
Y
Y
D000818:Animals; D000956:Antigens, Viral / Q000302:isolation & purification; D002460:Cell Line; D003715:Dengue / Q000175:diagnosis; D003716:Dengue Virus / Q000276:immunology / Q000302:isolation & purification*; D003906:Developing Countries; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006801:Humans; D014776:Virus Cultivation / Q000379:methods
D000956:Antigens, Viral