Glutathione S-transferases in Fasciola hepatica.
Glutathione S-transferases (GST's) are widespread in the tissues of the liver fluke, Fasciola hepatica, and consist of multiple isozymes. Following purification to apparent homogeneity by affinity chromatography on glutathione agarose, fluke GST's were shown to comprise 2 components with molecular weights of about 25,000. Fluke GST's were immunogenic to rats, but when used as a vaccine conferred no protection on the animals against a challenge infection with F. hepatica metacercariae.
Howell
M J
MJ
Department of Zoology, Australian National University, Canberra, ACT.
Board
P G
PG
Boray
J C
JC
D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis; D002846:Chromatography, Affinity; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D004592:Electrophoresis, Starch Gel; D005210:Fasciola hepatica / Q000201:enzymology*; D005211:Fascioliasis / Q000517:prevention & control; D005455:Fluorescent Antibody Technique; D005982:Glutathione Transferase / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification; D007118:Immunoassay; D007527:Isoenzymes / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D014611:Vaccination
D000909:Antibodies, Helminth; D007527:Isoenzymes; D005982:Glutathione Transferase