title: Human sperm carbohydrate antigens defined by an antisperm human monoclonal antibody derived from an infertile woman bearing antisperm antibodies in her serum.
abstract: The epitope structure of the human sperm antigen reacting with antibodies present in sera of infertile women has been studied using mAb H6-3C4, which produces immobilization of human sperm in the presence of complement. Another antibody, NUH2, which also induces human sperm immobilization, was used to substantiate the presence of a receptor on sperm involved in susceptibility to immobilization. Both antibodies defined type 2 chain polylactosamine structure. H6-3C4 is directed to internally located repetitive N-acetyllactosamine, i.e., sialyl-i, i, or fucosyl-i. NUH2 defines binary alpha 2----3 sialyl type 2 chain, i.e., sialyl-I. Thus, the presence of antibodies in the sera of infertile women directed to sperm lactosaminoglycan or lactosaminolipid could be the basis for infertility in these cases.
authors.affiliation: Biomembrane Institute, University of Washington, Seattle 98119.
authors.forename: Y
authors.initials: Y
authors.lastname: Tsuji
authors.forename: H
authors.initials: H
authors.lastname: Clausen
authors.forename: E
authors.initials: E
authors.lastname: Nudelman
authors.forename: T
authors.initials: T
authors.lastname: Kaizu
authors.forename: S
authors.initials: S
authors.lastname: Hakomori
authors.forename: S
authors.initials: S
authors.lastname: Isojima
mesh_terms: D000606:Amino Sugars / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D000941:Antigens / Q000276:immunology*; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000276:immunology*; D002855:Chromatography, Thin Layer; D003165:Complement System Proteins / Q000276:immunology; D005260:Female; D005455:Fluorescent Antibody Technique; D006384:Hemagglutination; D006801:Humans; D007118:Immunoassay; D007247:Infertility, Female / Q000276:immunology*; D008297:Male; D008969:Molecular Sequence Data; D011863:Radioimmunoassay; D013081:Sperm Motility; D013094:Spermatozoa / Q000276:immunology*
chemical_list: D000606:Amino Sugars; D000911:Antibodies, Monoclonal; D000941:Antigens; D002241:Carbohydrates; C000458:N-acetyllactosamine; D003165:Complement System Proteins