The radiation sensitivity of the haemopoietic microenvironment--effect of dose rate on ectopic ossicle formation.
The haemopoietic microenvironment (HM) consists of a complex mixture of cellular types and extra-cellular matrix. It is essential for prolonged haemopoiesis in both the normal situation and after bone marrow transplantation. The competence of the HM can be assessed by ectopic grafting of femoral marrow. A complete haemopoietic organ develops at the site of implantation. Stem cells (CFU-S) which inhabit the ossicle formed after ectopic implantation can be measured, to assess the function of the engrafted HM to support haemopoiesis. Using this functional endpoint we have examined the radiation sensitivity of the HM at both high and low dose rates, and conclude that high doses of gamma-irradiation delivered at 4 Gy/min or 0.016 Gy/min have widely different effects on the HM, the former proving much more damaging than the latter.
Molineux
G
G
Department of Experimental Haematology, Paterson Institute for Cancer Research, Christie Hospital and Holt Radium Institute, Withington, Manchester, U.K.
Testa
N G
NG
Hendry
J H
JH
Schofield
R
R
D000818:Animals; D001853:Bone Marrow / Q000528:radiation effects; D016026:Bone Marrow Transplantation; D003114:Colony-Forming Units Assay; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays; D006412:Hematopoietic Stem Cells / Q000528:radiation effects; D006413:Hematopoietic System / Q000528:radiation effects*; D007668:Kidney / Q000601:surgery; D051379:Mice; D010012:Osteogenesis / Q000528:radiation effects*; D011836:Radiation Tolerance; D014916:Whole-Body Irradiation