Factors affecting the production of amphotericin A.
Factors affecting amphotericin A synthesis of Streptomyces nodosus, NDMC-034 were studied. Iron, magnesium and manganese were found to stimulate amphotericin A synthesis at concentrations ranging from 10-100 microM. The optimum inoculum size, and the pH of production medium before sterilization for producing amphotericin A, were found to be near 10% (v/v) and pH 7.8, respectively. Carrying out fermentation in a complex medium using pharmamedia as nitrogen source resulted in an amphotericin A yield of up to 3.4 g/liter. A novel isolation and purification process for amphotericin A from the fermentation broth was developed, using an extracting isopropyl alcohol and methanolic solution containing 2% CaCl2. Amphotericin A exhibits a much lower acute toxicity in mice than amphotericin B.
Liu
Y T
YT
Institute of Microbiology, National Defense Medical Center, Taipei, ROC.
Wu
W L
WL
Chiang
M H
MH
Hu
S J
SJ
D000666:Amphotericin B / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000494:pharmacology / Q000633:toxicity; D000818:Animals; D002176:Candida albicans / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D005285:Fermentation; D008297:Male; D051379:Mice; D013302:Streptomyces / Q000378:metabolism
C041989:amphotericin A; D000666:Amphotericin B