Improved preparation of semisynthetic PheB1-tritiated insulin.
Semisynthetic [PheB1-3H]insulin was prepared. Each intermediate in the synthesis was characterised by HPLC, polyacrylamide gel electrophoresis and end group determination by dansylation. The end product had a specific activity of up to 59 Ci/mmol. Semisynthetic insulin proved indistinguishable from natural insulin in its chemical and immunological properties and was shown to be biologically fully active by in vivo and in vitro assays.
Grant
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von Holt
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D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D003619:Dansyl Compounds; D004591:Electrophoresis, Polyacrylamide Gel; D007328:Insulin / Q000494:pharmacology; D007553:Isotope Labeling / Q000379:methods*; D051379:Mice; D010649:Phenylalanine; D014316:Tritium
D001786:Blood Glucose; D003619:Dansyl Compounds; D007328:Insulin; D014316:Tritium; D010649:Phenylalanine