title: Signal sequence mutations that alter coupling of secretion and translation of an Escherichia coli outer membrane protein.
abstract: The lamB701-708 signal sequence mutation reduces expression of LamB, an outer membrane protein of Escherichia coli. To investigate the possibility that synthesis and export of LamB are coupled, as suggested by the expression defect of the lamB701-708 mutation, we isolated intragenic suppressors of the lamB701-708 mutation. The expression defect imposed by the lamB701-708 mutation is suppressed by an export-defective signal sequence mutation, suggesting that translation and export are coupled. The additional observation that not all export-defective signal sequence mutations suppressed the lamB701-708 expression defect suggests that translational arrest can be uncoupled from export.
authors.affiliation: Department of Molecular Biology, Princeton University, New Jersey 08544.
authors.forename: S A
authors.initials: SA
authors.lastname: Benson
authors.forename: M N
authors.initials: MN
authors.lastname: Hall
authors.forename: B A
authors.initials: BA
authors.lastname: Rasmussen
mesh_terms: D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D004269:DNA, Bacterial / Q000032:analysis; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation; D005798:Genes, Bacterial; D009154:Mutation; D010641:Phenotype; D014176:Protein Biosynthesis; D021382:Protein Sorting Signals / Q000235:genetics*; D013489:Suppression, Genetic
chemical_list: D001425:Bacterial Outer Membrane Proteins; D004269:DNA, Bacterial; D021382:Protein Sorting Signals