title: Changes in the cell surface properties of Treponema pallidum that occur during in vitro incubation of freshly extracted organisms.
abstract: We previously reported that a number of Treponema pallidum membrane proteins appear to reside on the cell surface, since intact treponemes radiolabeled by overnight incubation in medium containing [35S]methionine bind immunoglobulin G (IgG) antibodies directed against these proteins. In the present study, it was found that freshly extracted organisms radiolabeled in vitro for only 2 h inefficiently bound IgG antibodies directed against just two proteins of molecular weights 40,000 and 34,000. An in vitro incubation period of greater than 8 h was required before IgG antibodies present in rabbit syphilitic serum could recognize additional protein antigens on the cell surface. Treatment of aged treponemes, but not freshly extracted organisms, with 0.04% sodium dodecyl sulfate selectively removed a membranous layer from the treponemal surface. Only three treponemal proteins were found associated with this structure, including the same 40,000- and 34,000-molecular-weight proteins mentioned above. These two proteins most likely represent endoflagellar subunits, since they were precipitated with rabbit antisera prepared against purified endoflagellar subunits of the cultivable treponemal strain Treponema phagedenis. Further evidence also was obtained that cells of T. pallidum actively secrete into their extracellular environment a unique class of low-molecular-weight proteins.
authors.forename: L V
authors.initials: LV
authors.lastname: Stamm
authors.forename: R L
authors.initials: RL
authors.lastname: Hodinka
authors.forename: P B
authors.initials: PB
authors.lastname: Wyrick
authors.forename: P J
authors.initials: PJ
authors.lastname: Bassford
mesh_terms: D000953:Antigens, Protozoan / Q000032:analysis*; D000954:Antigens, Surface / Q000032:analysis*; D005407:Flagella / Q000648:ultrastructure; D007074:Immunoglobulin G / Q000276:immunology; D008854:Microscopy, Electron; D008970:Molecular Weight; D011506:Proteins / Q000276:immunology; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D013499:Surface Properties; D013997:Time Factors; D014210:Treponema pallidum / Q000502:physiology* / Q000648:ultrastructure
chemical_list: D000953:Antigens, Protozoan; D000954:Antigens, Surface; D007074:Immunoglobulin G; D011506:Proteins; D012967:Sodium Dodecyl Sulfate