Evidence for the presence of mutagenic compounds other than chromium in particles from mild steel welding.
A modified Salmonella/microsome liquid culture assay was used to investigate the mutagenicity of the particulate fraction from mild steel welding. Previous reports have implicated compounds of chromium VI as the mutagenic and toxic agents in welding fumes, since only the particles from welding on stainless steel, which contains 15-25% chromium, were mutagenic, whereas particles from welding on mild steel, which contain less than 0.1% chromium, were not mutagenic or toxic. In this investigation, mild steel particles were shown to contain direct-acting and promutagenic compounds that induced frameshift mutations. The mutagenic agents, which were insoluble in sodium phosphate buffer, did not include chromium VI or organic compounds. Further, the expression of mutation appears to require a cell-particle interaction for the release of the mutagenic species from the particles.
Biggart
N W
NW
Rinehart
R R
RR
Verfaillie
J
J
D000332:Aerobiosis; D000336:Aerosols; D000497:Alloys; D000693:Anaerobiosis; D001711:Biotransformation; D003102:Colloids; D008670:Metals / Q000032:analysis; D008862:Microsomes, Liver / Q000378:metabolism; D009152:Mutagenicity Tests; D009153:Mutagens / Q000032:analysis; D012486:Salmonella typhimurium / Q000187:drug effects; D013232:Steel; D014896:Welding
D000336:Aerosols; D000497:Alloys; D003102:Colloids; D008670:Metals; D009153:Mutagens; D013232:Steel