title: [Identification of idiotrophs for 3'-deoxykanamycin B synthesis in Streptomyces tenebrarius (Higgens a. Kastner)].
abstract: Mutants of Streptomyces tenebrarius with the blocked synthesis of 3'-deoxykanamycin B were obtained by treating the producer with NTG and chloramphenicol, or after gamma-irradiation. These mutants (idiotrophs) were distributed into three groups by means of the cosynthesis experiments on agar plates: convertors, secretors and "neutral" strains. Five idiotrophs represented five complementation groups for biosynthesis of the antibiotic. Three of these were defective in 2-deoxystreptamine synthesis, the fourth was defined as neamine-negative, and the fifth was probably blocked in regulation of enzymes responsible for conversion of neamine or paromamine into kanamycins. Localization of mutations has been shown on the scheme of kanamycins' biosynthesis.
authors.forename: Iu D
authors.initials: IuD
authors.lastname: Vikhanskiĭ
authors.forename: Iu V
authors.initials: IuV
authors.lastname: Zhdanovich
authors.forename: Iu E
authors.initials: IuE
authors.lastname: Bartoshevich
mesh_terms: D001431:Bacteriological Techniques; D007612:Kanamycin / Q000031:analogs & derivatives / Q000096:biosynthesis; D009154:Mutation; D013302:Streptomyces / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D014031:Tobramycin / Q000096:biosynthesis
chemical_list: C001495:bekanamycin; D007612:Kanamycin; D014031:Tobramycin
vernacular_title: Identifikatsiia idiotrofov po sintezu 3'-dezoksikanamitsina B u Streptomyces tenebrarius (Higgens a. Kastner).