title: Regulation of urokinase-type plasminogen activator production by cultured human cytotrophoblasts.
abstract: Cultured human cytotrophoblasts synthesize and secrete urokinase-type plasminogen activator (uPA) during the first 24 h of culture, but secretion declines during the subsequent day. In contrast, synthesis and secretion of fibronectin increases during the 2 days of culture. The levels of uPA mRNA parallel the changes in synthesis and secretion of uPA. Treatment of cytotrophoblasts with 8-bromo-cAMP (1.5 mM) transiently raises uPA mRNA levels and uPA secretion. This treatment reduces fibronectin mRNA levels and causes a sustained increase in beta chorionic gonadotropin mRNA content and chorionic gonadotropin secretion. We conclude that a cAMP-mediated process up-regulates uPA expression in cytotrophoblasts. However, the stimulatory effect of the cyclic nucleotide analog on uPA is transient.
authors.forename: J T
authors.initials: JT
authors.lastname: Queenan
authors.forename: L C
authors.initials: LC
authors.lastname: Kao
authors.forename: C E
authors.initials: CE
authors.lastname: Arboleda
authors.forename: A
authors.initials: A
authors.lastname: Ulloa-Aguirre
authors.forename: T G
authors.initials: TG
authors.lastname: Golos
authors.forename: D B
authors.initials: DB
authors.lastname: Cines
authors.forename: J F
authors.initials: JF
authors.lastname: Strauss
mesh_terms: D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology; D002478:Cells, Cultured; D005353:Fibronectins / Q000096:biosynthesis / Q000378:metabolism; D007163:Immunosorbent Techniques; D007700:Kinetics; D012333:RNA, Messenger / Q000378:metabolism; D014327:Trophoblasts / Q000187:drug effects / Q000201:enzymology*; D014568:Urokinase-Type Plasminogen Activator / Q000096:biosynthesis* / Q000378:metabolism
chemical_list: D005353:Fibronectins; D012333:RNA, Messenger; D015124:8-Bromo Cyclic Adenosine Monophosphate; D014568:Urokinase-Type Plasminogen Activator