Positive interaction between insulin and phorbol esters on the regulation of a specific messenger ribonucleic acid in rat hepatoma cells.
With Northern blot analysis, we demonstrate that phorbol 12-myristate 13-acetate (PMA) stimulated P-33 messenger RNA (mRNA)-accumulation in rat hepatoma cells in a time- and concentration-dependent manner similar to insulin and plant lectins. No effect of PMA on P-33 mRNA half-life was detected when mRNA synthesis was inhibited with either actinomycin D or 5.6-dichloro-1-beta-D-ribofuranosyl benzimidazole. The effects of insulin and PMA were additive at submaximal concentrations and no additivity was observed under these conditions at maximal concentrations. Thus PMA has a marked insulin-like effect on the accumulation of P-33 mRNA in rat hepatoma cells.
Messina
J L
JL
Department of Physiology, State University of New York-Health Science Center at Syracuse 13210.
Hamlin
J
J
Larner
J
J
D000818:Animals; D002460:Cell Line; D004347:Drug Interactions; D004355:Drug Stability; D007328:Insulin / Q000494:pharmacology*; D008114:Liver Neoplasms, Experimental / Q000378:metabolism / Q000473:pathology; D009994:Osmolar Concentration; D012333:RNA, Messenger / Q000187:drug effects*; D051381:Rats; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D013997:Time Factors
D007328:Insulin; D012333:RNA, Messenger; D013755:Tetradecanoylphorbol Acetate