[Serological reaction systems using enzyme-labelled immunospecific reagents].
Different serological test systems, based on the use of enzyme-labeled immunospecific reagents and intended for testing the material under study for the presence of Yersinia pestis capsular antigen and antibodies to it, are described. Comparative data on the evaluation of their sensitivity to the antigen and antibodies to it in different schemes of enzyme immunoassays (EIA) are presented. As shown in this investigation, EIA systems for the detection of the antigen and antibodies to it can comprise, at the minimum, the following set of reagents: monoclonal antibodies to the capsular antigen, staphylococcal protein A, and the conjugates of the capsular antigen and monoclonal antibodies with horse-radish peroxidase. The authors have come to the conclusion that the use of the serological test systems can essentially increase the reliability of the assay of any individual sample by EIA techniques.
Vengerov
Iu Iu
IuIu
Levi
M I
MI
Volkov
D V
DV
Sveshnikov
P G
PG
Severin
E S
ES
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000302:isolation & purification; D000942:Antigens, Bacterial / Q000032:analysis / Q000276:immunology; D002138:Calibration; D005069:Evaluation Studies as Topic; D007124:Immunoenzyme Techniques / Q000295:instrumentation; D007202:Indicators and Reagents; D051379:Mice; D008807:Mice, Inbred BALB C; D012698:Serologic Tests / Q000295:instrumentation / Q000379:methods*; D015010:Yersinia pestis / Q000276:immunology
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D007202:Indicators and Reagents
Sistemy serologicheskikh reaktsiĭ s ispol'zovaniem immunospetsificheskikh reagentov, mechennykh fermentom.