Detection of antibodies to human immunodeficiency virus by latex agglutination with recombinant antigen.
Recombinant human immunodeficiency virus (HIV) env antigen was attached to polystyrene particles, and these complexes were used to develop the first latex agglutination assay for antibodies to HIV. A total of 95 positive and 116 negative human serum samples were assayed for antibodies to HIV by latex agglutination, and results were compared with those of a commercial enzyme immunoassay. Latex agglutination was also compared with, and found to be completely concordant with, Western blot (immunoblot) analysis with virion antigens.
Riggin
C H
CH
Cambridge BioScience Corporation, Worcester, Massachusetts 01605.
Beltz
G A
GA
Hung
C H
CH
Thorn
R M
RM
Marciani
D J
DJ
D000914:Antibodies, Viral / Q000032:analysis*; D000956:Antigens, Viral / Q000276:immunology*; D006678:HIV / Q000276:immunology*; D015483:HIV Antibodies; D015488:HIV Antigens; D006801:Humans; D007118:Immunoassay; D007124:Immunoenzyme Techniques; D007841:Latex Fixation Tests; D011237:Predictive Value of Tests; D011994:Recombinant Proteins / Q000276:immunology
D000914:Antibodies, Viral; D000956:Antigens, Viral; D015483:HIV Antibodies; D015488:HIV Antigens; D011994:Recombinant Proteins