title: Sensitive methods for determining subclasses of IgG anti-A and anti-B in sera of blood-group-O women with a blood-group-A or -B child.
abstract: The determination of the subclasses of IgG antibodies against blood groups A and B is important in order to improve our understanding and predict haemolytic disease of the newborn due to IgG anti-A or -B. We describe two techniques that circumvent the problem of the agglutination of A and B red cells by the corresponding IgG antibodies in saline: an antiglobulin consumption test and a modified solid-phase micro-immunofluorescence test. The results of the two techniques are compared with the results obtained in the indirect antiglobulin test beyond the saline agglutination titre in a microplate technique. The solid-phase micro-immunofluorescence test was the most sensitive for the determination of the subclasses of IgG anti-A and -B. Usually sera contained IgG2 anti-A, B in a higher titre than antibodies of other subclasses.
authors.forename: H A
authors.initials: HA
authors.lastname: Brouwers
authors.forename: M A
authors.initials: MA
authors.lastname: Overbeeke
authors.forename: R J
authors.initials: RJ
authors.lastname: Gemke
authors.forename: C J
authors.initials: CJ
authors.lastname: Maas
authors.forename: E F
authors.initials: EF
authors.lastname: van Leeuwen
authors.forename: C P
authors.initials: CP
authors.lastname: Engelfriet
mesh_terms: D000017:ABO Blood-Group System / Q000276:immunology*; D003298:Coombs Test; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007074:Immunoglobulin G / Q000145:classification*; D007231:Infant, Newborn; D007518:Isoantibodies / Q000145:classification*; D011247:Pregnancy / Q000276:immunology*
chemical_list: D000017:ABO Blood-Group System; D007074:Immunoglobulin G; D007518:Isoantibodies