[Quality control of multicenter evaluation studies of the in vitro activity of antibiotics].
The estimation of the in vitro activity of an antibiotic, by different laboratories, requires the measurement, in standardized conditions, of the inhibition zone diameters versus a series of strains distributed to these laboratories (kits). Using the studies realized with two antibiotics, denoted by A and B, we observed an important variability between the different laboratories for a given strain and a less but still significant variability within the laboratories. Variance analysis revealed a significant interaction between strains and experimental laboratories. These results prevent the establishment of a standard diameter for a given strain but permit the quality control of the measurements and the improvement of the experimental design for future studies.
Tekaia
F
F
Unité d'Informatique Scientifique, Institut Pasteur, Paris.
Derlot
E
E
Claverie
J M
JM
Courvalin
P
P
D000704:Analysis of Variance; D008826:Microbial Sensitivity Tests / Q000592:standards*; D008954:Models, Biological; D011786:Quality Control
Contrôle de qualité des études multicentriques d'évaluation de l'activité in vitro des antibiotiques.