title: [Limited proteolysis of prolactin in secretory granules from the pituitary gland of estrogenized rats].
abstract: The LTH-converting proteolytic activity in LTH granules isolated from estrogenized rat hypophysis was studied. Suspensions of granules were incubated at different values of pH for 4 hours at 37 degrees C. The reaction was controlled by SDS electrophoresis. Intensive proteolysis of LTH was observed at pH 6.0 and 3.9, which was accompanied by the formation of fragments with Mr 10, 12 and 17 kD and probably of smaller peptides. An inhibitory analysis revealed that the formation of the 17 kD fragment at pH 3.9 was partly and selectively inhibited by chloroquine, phenanthroline and phenylmethylsulfonyl fluoride. Pepstatin A fully inhibited the proteolysis, whereas leupeptin had no inhibiting influence. The data obtained testify to the presence in the granular fraction of the endopeptidase LTH-converting activity which is sensitive to pepstatin A, an aspartyl proteinase inhibitor as well as to chelators and a serine proteinase inhibitor.
authors.forename: T V
authors.initials: TV
authors.lastname: Kuznetsova
authors.forename: V A
authors.initials: VA
authors.lastname: Tishchenko
authors.forename: L V
authors.initials: LV
authors.lastname: Nagornaia
authors.forename: O G
authors.initials: OG
authors.lastname: Krivosheev
authors.forename: G M
authors.initials: GM
authors.lastname: Kravtsov
mesh_terms: D000818:Animals; D003594:Cytoplasmic Granules / Q000378:metabolism*; D004054:Diethylstilbestrol / Q000008:administration & dosage*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006868:Hydrolysis; D008970:Molecular Weight; D010446:Peptide Fragments / Q000378:metabolism; D010447:Peptide Hydrolases / Q000378:metabolism; D010903:Pituitary Gland, Anterior / Q000378:metabolism* / Q000648:ultrastructure; D011388:Prolactin / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D010446:Peptide Fragments; D004054:Diethylstilbestrol; D011388:Prolactin; D010447:Peptide Hydrolases
vernacular_title: Ogranichennyĭ proteoliz prolaktina v sekretornykh granulakh iz gipofiza éstrogenizirovannykh krys.