title: Standardization of procedures for ectopic marrow grafting. II. Influence on recipients of radiation dose and field size.
abstract: The ectopic implantation of mouse marrow to the kidney capsule offers considerable scope as an assay of the hemopoietic microenvironment. Our previous work has shown that whole-body irradiation of the graft recipient prior to implantation results in superior ossicle formation in the kidney of the host. Here we report that a range of irradiation doses over a 4-Gy threshold are equivalent with respect to conditioning the graft recipient. We also show that two distinct and separable influences affect graft growth in the irradiated recipient, namely, a local effect brought about in the irradiated kidney (and restricted to it) and secondly, a systemic effect resulting from irradiation of sites other than the kidney, which nevertheless affects ossicle growth in the shielded renal capsule.
authors.forename: G
authors.initials: G
authors.lastname: Molineux
authors.forename: R
authors.initials: R
authors.lastname: Schofield
authors.forename: J H
authors.initials: JH
authors.lastname: Hendry
authors.forename: N G
authors.initials: NG
authors.lastname: Testa
mesh_terms: D000818:Animals; D016026:Bone Marrow Transplantation; D003114:Colony-Forming Units Assay / Q000379:methods; D004307:Dose-Response Relationship, Radiation; D005260:Female; D006412:Hematopoietic Stem Cells / Q000528:radiation effects; D007668:Kidney / Q000528:radiation effects*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D012737:Sex Factors; D014180:Transplantation / Q000379:methods; D014916:Whole-Body Irradiation