Regulation of urokinase-type plasminogen activator production by cultured human cytotrophoblasts.
Cultured human cytotrophoblasts synthesize and secrete urokinase-type plasminogen activator (uPA) during the first 24 h of culture, but secretion declines during the subsequent day. In contrast, synthesis and secretion of fibronectin increases during the 2 days of culture. The levels of uPA mRNA parallel the changes in synthesis and secretion of uPA. Treatment of cytotrophoblasts with 8-bromo-cAMP (1.5 mM) transiently raises uPA mRNA levels and uPA secretion. This treatment reduces fibronectin mRNA levels and causes a sustained increase in beta chorionic gonadotropin mRNA content and chorionic gonadotropin secretion. We conclude that a cAMP-mediated process up-regulates uPA expression in cytotrophoblasts. However, the stimulatory effect of the cyclic nucleotide analog on uPA is transient.
Queenan
J T
JT
Kao
L C
LC
Arboleda
C E
CE
Ulloa-Aguirre
A
A
Golos
T G
TG
Cines
D B
DB
Strauss
J F
JF
D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology; D002478:Cells, Cultured; D005353:Fibronectins / Q000096:biosynthesis / Q000378:metabolism; D007163:Immunosorbent Techniques; D007700:Kinetics; D012333:RNA, Messenger / Q000378:metabolism; D014327:Trophoblasts / Q000187:drug effects / Q000201:enzymology*; D014568:Urokinase-Type Plasminogen Activator / Q000096:biosynthesis* / Q000378:metabolism
D005353:Fibronectins; D012333:RNA, Messenger; D015124:8-Bromo Cyclic Adenosine Monophosphate; D014568:Urokinase-Type Plasminogen Activator