[Electrostimulated cell fusion in cell engineering].
A survey of studies on reconstructions of animal and plant cells which apply a new physical method--electrostimulated fusion, is presented. Effects of different factors of the medium on the efficiency of electrofusion is discussed. A detailed account is given of the authors' studies on zygotes reconstruction by combined methods of microsurgery and electrostimulated cell fusion. Advantages of the latter as compared to the widely distributed methods of fusion by polyethylenglycol and Sendai virus are considered. This physical method can play an important role in the progress of cellular engineering.
Chaĭlakhian
L M
LM
Veprintsev
B N
BN
Sviridova
T A
TA
Nikitin
V A
VA
D000818:Animals; D002459:Cell Fusion; D004558:Electric Stimulation; D005818:Genetic Engineering; D006801:Humans
Elektrostimuliruemoe sliianie kletok v kletochnoĭ inzhenerii.