An in vitro slice preparation of the cat lateral geniculate nucleus.
A slice preparation of the cat thalamus containing the lateral geniculate nucleus and the terminal portion of the optic tract is described. Ultrastructurally the slices remain relatively normal for only a short time after cutting. Indeed most cellular elements deteriorate quickly with time but patches of relatively intact tissue were still present even 10 h after cutting and maintenance in a storage bath. However, for 4-5 h after cutting long-lasting intracellular recordings of high quality and stability were obtained, and intrasomatic injection of horseradish peroxidase used for the morphological identification of recorded neurones as X or Y cells.
Crunelli
V
V
Leresche
N
N
Hynd
J W
JW
Patel
N M
NM
Parnavelas
J G
JG
D000818:Animals; D002415:Cats; D004594:Electrophysiology; D005829:Geniculate Bodies / Q000502:physiology* / Q000648:ultrastructure; D006652:Histological Techniques; D006735:Horseradish Peroxidase; D066298:In Vitro Techniques; D008839:Microelectrodes; D008854:Microscopy, Electron; D013569:Synapses / Q000648:ultrastructure
D006735:Horseradish Peroxidase