title: Fluorescence and chemiluminescence enzyme immunoassays of 17 alpha-hydroxyprogesterone in dried blood spotted on filter paper.
abstract: Enzyme immunoassays for 17 alpha-hydroxyprogesterone (17-OHP) were developed. Horseradish peroxidase (HRP), glucose oxidase (GOD), invertase (INV) and glucose-6-phosphate dehydrogenase (G6PDH) were used as label enzymes. Double antibody coated beads or tubes were used for separating the bound and free fractions. Antisera used were prepared by using 4-carboxyethylthio-17-OHP and 3-carboxymethyl oxime-17-OHP-bovine serum albumin as immunogens. The bridge heterologous system was more sensitive than other site heterologous and homologous systems. The minimum amounts of 17-OHP detected were 0.25 and 1.0 pg/tube for fluorescence EIAs using HRP and GOD, and 0.1, 10 and 0.1 pg/tube for chemiluminescence EIAs using GOD, INV and G6PDH, respectively. The reproducibility and correlation with RIA were also studied. The present study demonstrates the feasibility of a neonatal screening for congenital adrenal hyperplasia.
authors.affiliation: School of Pharmaceutical Sciences, Showa University, Tokyo, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Tsuji
authors.forename: M
authors.initials: M
authors.lastname: Maeda
authors.forename: H
authors.initials: H
authors.lastname: Arakawa
authors.forename: S
authors.initials: S
authors.lastname: Shimizu
authors.forename: T
authors.initials: T
authors.lastname: Ikegami
authors.forename: Y
authors.initials: Y
authors.lastname: Sudo
authors.forename: H
authors.initials: H
authors.lastname: Hosoda
authors.forename: T
authors.initials: T
authors.lastname: Nambara
mesh_terms: D019326:17-alpha-Hydroxyprogesterone; D005455:Fluorescent Antibody Technique; D006801:Humans; D006908:Hydroxyprogesterones / Q000097:blood*; D007124:Immunoenzyme Techniques; D008163:Luminescent Measurements
chemical_list: D006908:Hydroxyprogesterones; D019326:17-alpha-Hydroxyprogesterone