Origin of serotonin innervation of the arcuate and ventromedial hypothalamic region.
Combined fluorescence serotonin immunohistochemistry and retrograde transport labelling with Fast blue and Fluoro-gold were used to identify serotonin-immunoreactive neurons in the midbrain and pons which project to the region of the arcuate and ventrome-dial hypothalamic nuclei. Approximately 90% of doubly labelled neurons were located in the 3 major mesencephalic serotonin-containing cell groups: dorsal raphe (38%), median raphe (21%) and medial lemniscus group (29%). Within these groups, there were numerous non-retrogradely labelled serotonin-immunoreactive neurons as well as numerous non-serotonin-immunoreactive retrogradely labelled neurons. No doubly labelled neurons were observed caudal to raphe pontis although non-serotonin-immunoreactive neurons were retrogradely labelled in the more caudal raphe nuclei.
Willoughby
J O
JO
Department of Medicine, Flinders Medical Centre, Flinders University of South Australia, Bedford Park.
Blessing
W W
WW
D000578:Amidines; D000818:Animals; D001111:Arcuate Nucleus of Hypothalamus / Q000032:analysis / Q000033:anatomy & histology* / Q000166:cytology; D005456:Fluorescent Dyes; D006651:Histocytochemistry; D007158:Immunologic Techniques; D008297:Male; D008636:Mesencephalon / Q000032:analysis / Q000033:anatomy & histology* / Q000166:cytology; D009434:Neural Pathways / Q000032:analysis / Q000166:cytology; D051381:Rats; D011919:Rats, Inbred Strains; D012701:Serotonin / Q000032:analysis* / Q000502:physiology; D014697:Ventromedial Hypothalamic Nucleus / Q000032:analysis / Q000033:anatomy & histology* / Q000166:cytology
D000578:Amidines; D005456:Fluorescent Dyes; C031455:diamidino compound 253-50; D012701:Serotonin