In vitro induction of myeloid surface markers in a rare case of acute leukemia.
The phenotypic marker profile of a case of acute leukemia is described; its immunophenotype is unique in that the blast cells were initially negative for a wide panel of monoclonal antibodies (McAbs) to surface and intracytoplasmic antigens and for the nuclear enzyme terminal deoxynucleotidyl transferase. Morphological and cytochemical examination suggested an acute myeloid leukemia (AML), but the cells were unreactive with anti-myeloid McAbs. Treatment with the phorbol ester 12-O-tetradecanoylphorbol 13-acetate (TPA) induced the cells to differentiate morphologically to macrophage-like cells and led to the expression of surface antigens that could be detected by antimyeloid McAbs. It is not clear whether these cells represent a rare subclass of leukemic cells that are void of any characteristic surface markers but have the potential to differentiate along the myeloid axis, or whether the antigens were masked by an unknown process.
Drexler
H G
HG
Coustan-Smith
E
E
Gignac
S M
SM
Jani
H
H
Hoffbrand
A V
AV
D000208:Acute Disease; D000368:Aged; D000911:Antibodies, Monoclonal / Q000276:immunology; D000951:Antigens, Neoplasm / Q000032:analysis / Q000276:immunology; D000954:Antigens, Surface / Q000032:analysis / Q000276:immunology; D001752:Blast Crisis; D002478:Cells, Cultured; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006801:Humans; D006824:Hybridization, Genetic; D007938:Leukemia / Q000175:diagnosis / Q000276:immunology*; D015470:Leukemia, Myeloid, Acute / Q000175:diagnosis; D016256:Lewis X Antigen / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology / Q000473:pathology; D008297:Male; D009439:Neuraminidase / Q000494:pharmacology; D010641:Phenotype; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D016256:Lewis X Antigen; D009439:Neuraminidase; D013755:Tetradecanoylphorbol Acetate