title: Comparison of the effects on purified Leydig cells of four hormones (oxytocin, vasopressin, opiates and LHRH) with suggested paracrine roles in the testis.
abstract: Four hormones have been identified by various authors as possible paracrine regulators of testicular Leydig cells. The aim of this study was to evaluate their effects on purified adult rat Leydig cells under various conditions in vitro, and then to assess whether comparable effects occurred in vivo. In agreement with previous findings, an LHRH agonist (LHRH-A) exerted clear-cut effects on testosterone secretion by Leydig cells both in vitro and in vivo. On its own, LHRH-A stimulated testosterone production by Leydig cells for up to 24 h in culture but inhibited testosterone production stimulated by human chorionic gonadotrophin (hCG) between 24 and 72 h of culture. In-vivo, unilateral intratesticular injection of adult rats with 1 ng LHRH-A resulted 5 h later in a significant increase in testosterone concentrations in testicular interstitial fluid (IF). Vasopressin exerted effects in vitro which were similar to those of LHRH-A. On its own, vasopressin stimulated testosterone production for up to 5 h of culture, but not thereafter, while in the presence of hCG, vasopressin inhibited testosterone production beyond 24 h of culture. The initial stimulatory effect of vasopressin on testosterone production occurred with concentrations of 1 nmol/l and higher, but the magnitude of stimulation (threefold or less) was considerably less than that induced by LHRH-A (ninefold) over the same time period. In contrast to LHRH-A, unilateral intratesticular injection of vasopressin in high doses (20 and 2 ng) had no effect on IF testosterone levels 5 h later.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.forename: R M
authors.initials: RM
authors.lastname: Sharpe
authors.forename: I
authors.initials: I
authors.lastname: Cooper
mesh_terms: D000818:Animals; D007987:Gonadotropin-Releasing Hormone / Q000494:pharmacology; D007985:Leydig Cells / Q000187:drug effects / Q000502:physiology*; D008297:Male; D009294:Narcotics / Q000494:pharmacology; D009842:Oligopeptides / Q000494:pharmacology*; D010121:Oxytocin / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D013739:Testosterone / Q000378:metabolism*; D014667:Vasopressins / Q000494:pharmacology
chemical_list: D009294:Narcotics; D009842:Oligopeptides; D014667:Vasopressins; D007987:Gonadotropin-Releasing Hormone; D013739:Testosterone; D010121:Oxytocin