Using SIB and API kits to identify and biotype enterobacteria of different origin.
A collection of 26 Enterobacteriaceae reference strains provided by Reference Centres in Moscow (USSR) and Copenhagen (Denmark) as well as a collection of 660 freshly isolated cultures of Gram-negative bacteria of different origin were investigated using SIB indicator systems manufactured at the Gorky Institute of Epidemiology and Microbiology (USSR) and API-20E, Rapid-20E and API-10S kits (API, France) with the aim of species determination. In analyzing freshly isolated cultures, API-20E, API-10S and SIB-B kits proved to be of approximately equal efficiency, whereas the Rapid-20E system enabled species identification in no more than 78% of the tested cultures. In a model biotyping of 284 E. coli cultures of different origin, SIB-B and API-20E kits in combination with the Analytical Profile Index enabled sufficiently rapid and standard identification of Enterobacteriaceae biovars.
Kulinich
L I
LI
Laboratory of Immunochemistry, All-Union Research Institute of Protein Substances Biosynthesis, Moskva, USSR.
Fish
N G
NG
Mazitova
O P
OP
Dratvin
S A
SA
Birger
M O
MO
D001431:Bacteriological Techniques; D004755:Enterobacteriaceae / Q000145:classification* / Q000302:isolation & purification; D004756:Enterobacteriaceae Infections / Q000175:diagnosis*; D006801:Humans; D011933:Reagent Kits, Diagnostic
D011933:Reagent Kits, Diagnostic