Insulin-modulated interleukin-2 production by murine splenocytes and a T-cell hybridoma.
Murine interleukin-2 (IL-2)-producing AOFS 21 T-cell hybridoma cells and normal murine splenocytes were stimulated in serum-free media by 16 potential mitogens/growth factors. Only insulin, concanavalin A (Con A), peptidoglycan monomer and a tumour-derived insulinoid stimulated [3H]thymidine incorporation by AOFS 21 cells and splenocytes. Supernatants of these stimulated cultures were tested for IL-2 activity which generally followed the pattern of growth stimulation. Both the mitogenicity and stimulation of IL-2 secretion by insulin were second only to the effects of Con A.
Pavelić
K
K
Grace Cancer Drug Center, Roswell Park Memorial Institute, Buffalo, New York 14263.
Bernacki
R J
RJ
Vuk-Pavlović
S
S
D000818:Animals; D002478:Cells, Cultured; D006133:Growth Substances / Q000494:pharmacology; D006825:Hybridomas / Q000378:metabolism; D007328:Insulin / Q000494:pharmacology*; D007376:Interleukin-2 / Q000378:metabolism*; D008297:Male; D051379:Mice; D008934:Mitogens / Q000494:pharmacology; D013154:Spleen / Q000378:metabolism*; D013601:T-Lymphocytes / Q000378:metabolism*
D006133:Growth Substances; D007328:Insulin; D007376:Interleukin-2; D008934:Mitogens