Three monoclonal antibodies with specific binding activity to the excretory system of Schistosoma mansoni: an immunoelectron microscopic study using the gold labeling technique.
This study describes three monoclonal antibodies against the excretory system of Schistosoma mansoni. Immunofluorescence revealed antigens forming part of the excretory system of cercariae, adult worms, and miracidia, which were located on the luminal membranes of flame and first tubule cells by immunoelectron microscopy. These antigens are either structural components of the membranes or they derive from excretory fluid and are absorbed during transport and ultrafiltration. Binding specificity of the monoclonal antibodies was tested by immunoelectrophoresis and competitive immunofluorescence; one or two antigens were recognized by each. Reactivity of the antigens after treatment with 7.5% trichloroacetic acid or Rossman's fixative demonstrates at least partial polysaccharide content.
de Water
R
R
Laboratory for Parasitology, University of Leiden, The Netherlands.
Fransen
J A
JA
Schut
D W
DW
Deelder
A M
AM
D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D005455:Fluorescent Antibody Technique; D006046:Gold; D007122:Immunoelectrophoresis; D007158:Immunologic Techniques; D008854:Microscopy, Electron; D012550:Schistosoma mansoni / Q000276:immunology* / Q000648:ultrastructure
D000909:Antibodies, Helminth; D000911:Antibodies, Monoclonal; D006046:Gold