title: Coexistence of pancreatic polypeptide (PP)-and glucagon-immunoreactivity in pancreatic endocrine cells of mouse.
abstract: Immunocytochemical double staining techniques were used to study PP- and glucagon-like-immunoreactivity in pancreatic endocrine cells of mouse. An antiserum against FMRFamide appeared to react with all PP-immunoreactive endocrine cells. With fluorescence microscopy most PP/FMRFamide-immunoreactive cells also showed glucagon-immunoreactivity, but cells containing only PP- or glucagon-like substances were found as well. The proportion of cells containing PP-, glucagon, and both immunoreactivities varied strongly from islet to islet in all parts of the pancreas. Using an electron microscopical immunogold double staining procedure on Lowicryl-embedded pancreas, PP/FMRFamide- and glucagon-immunoreactivity appeared to be present in the majority of endocrine A cells; both immunoreactivities were randomly distributed within the granules of these cells. Cells containing only PP/FMRFamide- or glucagon-immunoreactivity were also found. Glucagon- and a faint FMRFamide-immunoreactivity was also observed in osmicated epon-embedded tissue. Independent of their immunoreactivity all positive cells showed the same round electron dense secretory granules.
authors.forename: J H
authors.initials: JH
authors.lastname: Rombout
authors.forename: M E
authors.initials: ME
authors.lastname: Abad
authors.forename: F M
authors.initials: FM
authors.lastname: Binkhorst
authors.forename: J J
authors.initials: JJ
authors.lastname: Taverne-Thiele
mesh_terms: D000818:Animals; D019835:FMRFamide; D005934:Glucagon / Q000378:metabolism*; D006046:Gold; D007158:Immunologic Techniques; D007515:Islets of Langerhans / Q000378:metabolism*; D051379:Mice; D008854:Microscopy, Electron; D009479:Neuropeptides / Q000378:metabolism; D010191:Pancreatic Polypeptide / Q000378:metabolism*
chemical_list: D009479:Neuropeptides; D010191:Pancreatic Polypeptide; D019835:FMRFamide; D006046:Gold; D005934:Glucagon