Demonstration of pepsinogen C in human pancreatic islets.
Pancreatic tissue from 16 post mortem kidney donors have been examined for the content of pepsinogens. A zymogen with electrophoretic mobility, isoelectric point and molecular weight equal to that of pepsinogen C of gastric origin was found in all specimens. A comparison between pepsinogen C extracted from pancreatic tissue and gastric mucosa demonstrated immunological identity. Quantitative measurements with a radioimmunoassay showed pepsinogen C concentrations in pancreatic tissue three to 80 times higher than those of blood serum. Immunohistochemical staining gave positive reaction for pepsinogen C only in the alpha cells of the pancreatic islets.
Szecsi
P B
PB
Department of Clinical Chemistry, Hvidovre Hospital, Denmark.
Halgreen
H
H
Poulsen
S S
SS
Axelsson
C K
CK
Damkjaer-Nielsen
M
M
Kjaer
T
T
Foltmann
B
B
D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005753:Gastric Mucosa / Q000201:enzymology; D006801:Humans; D007124:Immunoenzyme Techniques; D007515:Islets of Langerhans / Q000032:analysis / Q000201:enzymology*; D007525:Isoelectric Focusing; D010435:Pepsinogens / Q000032:analysis / Q000378:metabolism*
D010435:Pepsinogens