title: recA gene of Escherichia coli complements defects in DNA repair and mutagenesis in Streptomyces fradiae JS6 (mcr-6).
abstract: Streptomyces fradiae JS6 (mcr-6) is a mutant which is defective in repair of DNA damage induced by a variety of chemical mutagens and UV light. JS6 is also defective in error-prone (mutagenic) DNA repair (J. Stonesifer and R. H. Baltz, Proc. Natl. Acad. Sci. USA 82:1180-1183, 1985). The recA gene of Escherichia coli, cloned in a bifunctional vector that replicates in E. coli and Streptomyces spp., complemented the mutation in S. fradiae JS6, indicating that E. coli and S. fradiae express similar SOS responses and that the mcr+ gene product of S. fradiae is functionally analogous to the protein encoded by the recA gene of E. coli.
authors.affiliation: Department of Molecular Genetics, Eli Lilly and Company, Indianapolis, Indiana 46285.
authors.forename: P
authors.initials: P
authors.lastname: Matsushima
authors.forename: R H
authors.initials: RH
authors.lastname: Baltz
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D003001:Cloning, Molecular; D004249:DNA Damage; D004260:DNA Repair; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D009154:Mutation; D011938:Rec A Recombinases / Q000235:genetics*; D013014:SOS Response, Genetics; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism; D014169:Transformation, Bacterial
chemical_list: D001426:Bacterial Proteins; D011938:Rec A Recombinases