[Conditions for efficient antibody binding with proteins of the human immunodeficiency virus studied by immunoblotting].
The authors have investigated the antibody binding with individual HIV protein by "western" blotting method. The optimal condition for binding was incubation of nitrocellulose strips at 37 degrees C for 2 hrs followed by incubation at 4 degrees C overnight. Differences in the serologic activities of a number of sera in "western" blotting were shown by using two different antigens. It was concluded that mixing of different antigens before electrophoresis may be useful for more effective "western" blotting analysis.
Berezin
V E
VE
Zaĭdes
V M
VM
Zhdanov
V M
VM
D000163:Acquired Immunodeficiency Syndrome / Q000382:microbiology; D000914:Antibodies, Viral / Q000032:analysis / Q000378:metabolism*; D000918:Antibody Specificity; D001666:Binding Sites, Antibody; D002353:Carrier State / Q000382:microbiology; D006678:HIV / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D007118:Immunoassay / Q000379:methods; D007124:Immunoenzyme Techniques; D013696:Temperature; D013997:Time Factors; D014764:Viral Proteins / Q000032:analysis / Q000378:metabolism*; D014776:Virus Cultivation
D000914:Antibodies, Viral; D014764:Viral Proteins
Izuchenie usloviĭ éffektivnogo sviazyvaniia antitel s belkami virusa immunodefitsita cheloveka v immunoblote.