Serodiagnosis of rabies by dot immunobinding assay.
A dot immunobinding assay that uses inactivated antigen for the detection of rabies viral antibodies was compared with the rapid fluorescent focus inhibition test. Results of testing pre- and postvaccination sera from humans (n = 33) and canines (n = 22) were identical for both tests. Endpoint titers of positive sera also were approximately the same by both methods. When a mouse monoclonal antibody was used, the dot immunobinding assay antigen was shown to possess detectable rabies virus glycoprotein and core antigens.
Heberling
R L
RL
Kalter
S S
SS
Smith
J S
JS
Hildebrand
D G
DG
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D004285:Dogs; D006801:Humans; D007158:Immunologic Techniques; D011818:Rabies / Q000175:diagnosis*; D011819:Rabies Vaccines / Q000276:immunology; D011820:Rabies virus / Q000276:immunology*; D014611:Vaccination
D000914:Antibodies, Viral; D011819:Rabies Vaccines