Effect of bacterial growth-inhibiting ingredients on the Ames mutagenicity of medicinal herbs.
A solvent fractionation method was introduced to screen for mutagenicity in 10 medicinal herbs being consumed in Korea. The Ames mutagenicity test result of Scutellariae and Rhei was significantly increased by eliminating growth-inhibiting substances through solvent fractionation of the crude extract. It is suggested that a physicochemical pretreatment should reduce the false-negative results which are caused by the presence of growth-inhibiting substances in complex mixtures.
Lee
H K
HK
Toxicology Center, Korea Research Institute of Chemical Technology, Chungnam.
Kim
Y K
YK
Kim
Y H
YH
Roh
J K
JK
D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000494:pharmacology*; D001554:Benzene; D005591:Chemical Fractionation; D005188:False Negative Reactions; D006586:Hexanes; D007723:Korea; D000432:Methanol; D009152:Mutagenicity Tests; D010936:Plant Extracts / Q000032:analysis / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000032:analysis*; D012486:Salmonella typhimurium / Q000187:drug effects*; D012997:Solvents; D014867:Water
D000900:Anti-Bacterial Agents; D006586:Hexanes; D010936:Plant Extracts; D012997:Solvents; D014867:Water; C026385:n-hexane; D001554:Benzene; D000432:Methanol