Aspects of measurement and analysis of regulatory peptides.
Although almost all methods of mass measurement of regulatory peptides still depend on the high affinity antibody, the traditional Yalow and Berson radioimmunoassay technique is becoming outdated. Pure monoclonal antibodies allow excess antibody two site assay techniques with a variety of different labels (preferentially non-radioactive) of great sensitivity and speed. The large amounts of particular monoclonal antibodies available allow several different laboratories to use the same reagents and have increased comparability. Unfortunately many regulatory peptides exist in multiple molecular forms and attention must be paid to antibody region specificity. Improved methods of extraction of regulatory peptides from plasma tissue allow more accurate quantitation. New techniques for rapid high resolution chromatography make distinction of different molecular forms much easier than hitherto. Better education in techniques and/or attention to inter-assay standards are necessary to improve the comparability of regulatory peptide measurement in the future.
Burrin
J M
JM
Uttenthal
L O
LO
McGregor
G P
GP
Bloom
S R
SR
D000818:Animals; D000906:Antibodies; D000911:Antibodies, Monoclonal; D005455:Fluorescent Antibody Technique; D006801:Humans; D007124:Immunoenzyme Techniques; D009479:Neuropeptides / Q000032:analysis*; D011863:Radioimmunoassay / Q000379:methods
D000906:Antibodies; D000911:Antibodies, Monoclonal; D009479:Neuropeptides