HLA-B27 typing by use of flow cytofluorometry.
We describe the use of flow cytofluorometry to type lymphocytes for HLA-B27 antigens. We modified the cytotoxicity test of Terasaki and McClelland [Nature (London) 1964;204:998] for use with a flow cytofluorometer. In a second assay we used a commercially available monoclonal anti-HLA-B27 antibody and indirect immunofluorescence staining of cell surfaces. Although this antibody cross reacts with B7 and Bw22 antigens, the results for HLA-B27 and the cross-reacting antigens can be separated. Comparison of our results with those by a HLA-typing laboratory for 100 patients showed total agreement of the assigned typings.
Albrecht
J
J
Müller
H A
HA
D000911:Antibodies, Monoclonal; D002462:Cell Membrane / Q000276:immunology; D003429:Cross Reactions; D003601:Cytotoxicity Tests, Immunologic; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006680:HLA Antigens / Q000032:analysis*; D015796:HLA-B27 Antigen; D006650:Histocompatibility Testing; D006801:Humans; D008214:Lymphocytes / Q000276:immunology*; D011419:Propidium
D000911:Antibodies, Monoclonal; D006680:HLA Antigens; D015796:HLA-B27 Antigen; D011419:Propidium