Reovirus major capsid protein expressed in Escherichia coli.
A DNA copy of the open reading frame of the S4 gene of reovirus type 3 was cloned into a temperature-regulated bacterial expression vector. Induction at 42 degrees C resulted in the synthesis of a polypeptide that comigrated with virion capsid protein sigma 3 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and reacted with sigma 3-specific antisera. The protein was expressed in bacteria as insoluble aggregates that accumulated in polar inclusion bodies. Aggregated product also resulted when the expression system was manipulated to induce bacterial sigma 3 (b sigma 3) synthesis at temperatures below 42 degrees C. Various methods used to solubilize b sigma 3 did not yield the monomeric protein. The results indicate that sigma 3, the major surface component of reovirions, is expressed in transfected Escherichia coli as an aggregated, disulfide cross-linked protein.
Giantini
M
M
Roche Institute of Molecular Biology, Nutley, NJ 07110.
Shatkin
A J
AJ
D002213:Capsid / Q000235:genetics*; D036022:Capsid Proteins; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors; D007181:Inclusion Bodies, Viral; D011487:Protein Conformation; D016601:RNA-Binding Proteins; D012191:Retroviridae Proteins / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics
D036022:Capsid Proteins; D016601:RNA-Binding Proteins; D012191:Retroviridae Proteins; D014764:Viral Proteins; C053179:sigma protein 3, Reovirus