A complete cDNA sequence for the major epidermal growth factor binding protein in the male mouse submandibular gland.
The complete cDNA sequence of the major epidermal growth factor binding protein (EGF-BP), isolated from the mouse submandibular gland, has been determined. Oligonucleotide probes complementary to unique, nonconserved, regions of homogeneous preparations of EGF-BP were used to identify the correct cDNA clone from a male mouse submandibular gland cDNA library. The nucleotide sequence codes for a glandular kallikrein that is the main arginine esterase complexed with epidermal growth factor. The mRNA coding for this EGF-BP is estimated at 0.24% of the total mRNA of the adult male mouse submandibular gland, thus representing an abundant member of the kallikrein family in this tissue. In addition, the cDNA sequence defines a putative transcription start site. The reported cDNA sequence is clearly different from, and not an allelic form of, a previously reported cDNA sequence for EGF-BP. The present work reconciles conflicting information in the literature regarding the identity of EGF-BP.
Blaber
M
M
Department of Biological Chemistry, California College of Medicine, University of California, Irvine 92717.
Isackson
P J
PJ
Bradshaw
R A
RA
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000302:isolation & purification*; D010450:Endopeptidases / Q000235:genetics*; D007610:Kallikreins / Q000235:genetics; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D014176:Protein Biosynthesis; D012689:Sequence Homology, Nucleic Acid; D013363:Submandibular Gland / Q000378:metabolism*; D020840:Tissue Kallikreins
D004247:DNA; D010450:Endopeptidases; D007610:Kallikreins; C489120:Klk13 protein, mouse; D020840:Tissue Kallikreins; C489121:Klk1b22 protein, mouse; C489119:Klk9 protein, mouse; C053223:epidermal growth factor binding protein, mouse