Overproduction and preliminary X-ray characterization of aspartate aminotransferase from Escherichia coli.
The aspartate aminotransferase of Escherichia coli was overproduced in cells after genetic manipulation, and was crystallized from a polyethylene glycol solution, pH 7.0. The crystals obtained were of good quality and had diffractions extending beyond 2.4 A. The space group and unit cell dimensions were determined with a precession camera and a four-circle diffractometer to be C222(1), and a = 157.1 A, b = 85.5 A, and c = 79.7 A, respectively. Only one protein subunit is contained in an asymmetric unit.
Kamitori
S
S
Hirotsu
K
K
Higuchi
T
T
Kondo
K
K
Inoue
K
K
Kuramitsu
S
S
Kagamiyama
H
H
Higuchi
Y
Y
Yasuoka
N
N
Kusunoki
M
M
D001219:Aspartate Aminotransferases / Q000096:biosynthesis; D003460:Crystallization; D004926:Escherichia coli / Q000201:enzymology*; D014961:X-Ray Diffraction
D001219:Aspartate Aminotransferases