[Gastric cancer-associated antigen P42].
The corresponding antigen to monoclonal antibody PCI against gastric cancer cells was demonstrated as a protein sensitive to heating and easily degraded by proteinase. By western blotting, it was shown that the molecular weight of the antigen was 42 KD and the antigen was named P42. P42 could be shed into serum-free culture medium of the target cells and its activity be assayed in sera of the gastric cancer patients. Using inhibition test, the levels of P42-like substance in sera were measured. The results indicated that this level in the gastric cancer patients (26.53 +/- 23.11) was markedly higher than that in patients with benign gastric diseases (4.66 +/- 2.67) and controls (2.57 +/- 2.67). It is suggested that P42, a novel gastric cancer-associated antigen, be useful in the mass screening and supplementary in diagnosing gastric cancer.
Dong
Z W
ZW
Beijing Institute for Cancer Research.
Li
Z F
ZF
Wei
S M
SM
Wu
J
J
You
W C
WC
D000911:Antibodies, Monoclonal / Q000276:immunology; D000951:Antigens, Neoplasm / Q000032:analysis*; D010450:Endopeptidases; D006358:Hot Temperature; D006801:Humans; D013274:Stomach Neoplasms / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D010450:Endopeptidases