title: Cloning and sequencing of a carp beta s-crystallin cDNA.
abstract: The mRNAs were extracted from common carp (Cyprinus carpio) lenses, purified, reverse transcribed, dC tailed and cloned into Escherichia coli with pBR322 as vector. The cloning efficiency was around 1 X 10(7) colonies per micrograms of mRNA. A clone (pC20) was found by hybrid-arrested to contain the cDNA related to carp crystallins. However, comparison of the derived amino-acid sequence with bovine gamma-II and beta s-crystallins indicates that this carp crystallin sequence resembles closely the bovine beta s-crystallin and should be better classified as such except that this fish sequence does not contain the N-terminal 'arm' of four amino-acid residues present in bovine beta s-crystallin.
authors.affiliation: Institute of Biochemical Sciences, National Taiwan University, Taipei, China.
authors.forename: T
authors.initials: T
authors.lastname: Chang
authors.forename: W C
authors.initials: WC
authors.lastname: Chang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002347:Carps / Q000235:genetics*; D002417:Cattle; D003001:Cloning, Molecular; D003459:Crystallins / Q000235:genetics*; D003530:Cyprinidae / Q000235:genetics*; D004247:DNA / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D007908:Lens, Crystalline / Q000032:analysis; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D012333:RNA, Messenger / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid
chemical_list: D003459:Crystallins; D012333:RNA, Messenger; D004247:DNA