Retinal insulin receptors. 2. Characterization and insulin-induced tyrosine kinase activity in bovine retinal rod outer segments.
Bovine retinal rod outer segments (ROS) possess specific, high-affinity receptors for insulin. These receptors exhibit an insulin-stimulatable tyrosine-specific activity that is capable of phosphorylating the receptor's own beta-subunit and exogenous substrate. ROS insulin receptors exhibit heterogeneity in the apparent molecular weight of the receptor's alpha-subunit. In this regard, insulin receptors from this single cell type resemble insulin receptors obtained from whole retina, but are unlike receptors from brain and liver.
Waldbillig
R J
RJ
Laboratory of Retinal Cell and Molecular Biology, National Eye Institute, Bethesda, MD 20892.
Fletcher
R T
RT
Chader
G J
GJ
Rajagopalan
S
S
Rodrigues
M
M
LeRoith
D
D
D000818:Animals; D001921:Brain / Q000378:metabolism; D002417:Cattle; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation; D007328:Insulin / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008970:Molecular Weight; D010766:Phosphorylation; D010786:Photoreceptor Cells / Q000201:enzymology*; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D011972:Receptor, Insulin / Q000032:analysis*; D012374:Rod Cell Outer Segment / Q000201:enzymology*; D014443:Tyrosine / Q000378:metabolism
D007328:Insulin; D014443:Tyrosine; D011505:Protein-Tyrosine Kinases; D011972:Receptor, Insulin