title: Location and function of fruC, a gene involved in the regulation of fructose utilization by Escherichia coli.
abstract: Procedures are described for the selection of Escherichia coli mutants that constitutively take up and phosphorylate fructose, and convert it to fructose 1,6-bisphosphate. The phenotype of such mutants is described. The altered regulatory gene, fruC, is highly co-transducible with leu and other markers located at min 2 on the genome. In merozygotes, fruC+ is dominant to fruC. Mutants can be readily isolated that are fruC at 42 degrees C but fruC+ at 30 degrees C; moreover, the integration of a Tn10 transposon in the genome at min 2 converts fruC+ strains to fruC. It is therefore likely that the fruC+ regulatory gene specifies a repressor protein.
authors.affiliation: Department of Biochemistry, University of Cambridge, UK.
authors.forename: H L
authors.initials: HL
authors.lastname: Kornberg
authors.forename: C M
authors.initials: CM
authors.lastname: Elvin
mesh_terms: D000117:Acetylglucosamine / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D005632:Fructose / Q000235:genetics* / Q000378:metabolism; D005798:Genes, Bacterial; D005947:Glucose / Q000378:metabolism; D009154:Mutation; D013012:Sorbitol / Q000378:metabolism
chemical_list: D005632:Fructose; D013012:Sorbitol; D005947:Glucose; D000117:Acetylglucosamine