Immunological reclassification of 22 children with a former diagnosis of non-T, non-B ALL.
Stored peripheral blood or bone marrow mononuclear cells from 22 pediatric patients with verified acute lymphoblastic leukemia (ALL) previously classified as non-T, non-B ALL were re-investigated by flow cytometric analysis by means of a panel of B cell-specific and -associated monoclonal antibodies (moabs) using a new analytical method described by Platz et al, the so-called Delta Channel Value method. All 22 patients were immunologically re-characterized as pre-B ALL. The reproducibility between the first (acute) and subsequent re-analysis was almost complete. 20 of the tumor cell populations could be assigned to the B cell differentiation scheme recently proposed by Nadler et al. This scheme operates with four stages of pre-B cell differentiation and each stage is defined by the expression of one to four of the following markers: HLA-DR, CD19, CD10 and CD20. Two additional markers, CD24 and CD22, were investigated in our study and allowed further subdivision of the four subgroups proposed by Nadler et al. The composition of a panel of moabs for routine classification of pre-B ALL is proposed.
Fugger
L
L
Department of Clinical Immunology, Rigshospitalet, Copenhagen, Denmark.
Platz
P
P
Ryder
L
L
Svejgaard
A
A
Heldrup
J
J
Hertz
H
H
Yssing
M
M
D000293:Adolescent; D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007223:Infant; D007945:Leukemia, Lymphoid / Q000145:classification / Q000276:immunology*; D008297:Male
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm