Effect of DNA superhelicity on transcription termination.
Restriction fragments containing either leut (a rho-independent transcription termination site) and/or leut' (a rho-dependent transcription termination site) were cloned into plasmid pOL4. Treatment of plasmid-containing Escherichia coli strains with coumermycin resulted in loss of in vivo plasmid superhelicity 10 min after antibiotic addition. Galactokinase levels specified by these plasmid-containing strains were the same regardless of whether functional DNA gyrase was present. These results suggest that transcription termination is unaffected by the superhelical state of DNA.
Rosenthal
E R
ER
Calvo
J M
JM
D000123:Acetyltransferases / Q000235:genetics*; D049933:Aminocoumarins; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001483:Base Sequence; D015500:Chloramphenicol O-Acetyltransferase; D003374:Coumarins / Q000494:pharmacology; D004278:DNA, Superhelical / Q000235:genetics*; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D005686:Galactokinase / Q000235:genetics*; D010957:Plasmids; D014158:Transcription, Genetic
D049933:Aminocoumarins; D000900:Anti-Bacterial Agents; D003374:Coumarins; D004278:DNA, Superhelical; D000123:Acetyltransferases; D015500:Chloramphenicol O-Acetyltransferase; D005686:Galactokinase; C004628:coumermycin