Preoperative metabolic analysis of donor corneas using magnetic resonance spectroscopy.
Successful corneal transplantation was accomplished following metabolic phosphorus magnetic resonance analysis. Four cat corneas were analyzed using phosphorus-31 magnetic resonance following storage in modified McCarey-Kaufman (M-K) medium for 24 h. Corneas were re-stored in M-K medium and transplanted 24 h after MR analysis. Four control corneas (contralateral eye, no magnetic resonance analysis performed) were also transplanted following storage in M-K medium under identical conditions. Successful corneal transplantation was accomplished with minimal ATP tissue levels. Corneas stored for 48 h maintained a pH of 7.3. The phosphorus-31 spectral modulus, which is the ratio of the high-energy phosphates to the low-energy phosphates, was calculated using the spectral integral (range, 0.49-0.77). No difference in endothelial cell density or morphology was detected between corneas following magnetic resonance analysis and control corneas when evaluated by specular microscopy.
Lass
J H
JH
Division of Ophthalmology, Case Western Reserve Univerity, Cleveland, Ohio.
Medcalf
S K
SK
Greiner
J V
JV
Glonek
T
T
D000818:Animals; D002415:Cats; D003315:Cornea / Q000378:metabolism*; D003316:Corneal Diseases / Q000209:etiology; D016039:Corneal Transplantation; D004487:Edema / Q000209:etiology; D009682:Magnetic Resonance Spectroscopy / Q000009:adverse effects; D010758:Phosphorus / Q000378:metabolism*
D010758:Phosphorus