title: Characterization of protein kinase C from normal and transformed cultured murine fibroblasts.
abstract: Protein kinase C of normal and ras-transformed NIH 3T3 cells was purified by chromatography on TSK DEAE-5PW, threonine-Sepharose, and TSK phenyl-5PW columns. Comparison of the fibroblast enzyme with several types of rat brain protein kinase C by chromatography on a hydroxyapatite column and by immunoblotting, indicates that both normal and transformed fibroblasts possess only one of the four subspecies of protein kinase C which have been identified in brain tissues. This subspecies presumably has the structure encoded by alpha-sequence or a closely related sequence. No significant difference was seen between those enzymes purified from normal and transformed fibroblasts.
authors.forename: P G
authors.initials: PG
authors.lastname: McCaffrey
authors.forename: M R
authors.initials: MR
authors.lastname: Rosner
authors.forename: U
authors.initials: U
authors.lastname: Kikkawa
authors.forename: K
authors.initials: K
authors.lastname: Sekiguchi
authors.forename: K
authors.initials: K
authors.lastname: Ogita
authors.forename: K
authors.initials: K
authors.lastname: Ase
authors.forename: Y
authors.initials: Y
authors.lastname: Nishizuka
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D001921:Brain / Q000201:enzymology; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D002850:Chromatography, Gel; D005347:Fibroblasts / Q000201:enzymology*; D007163:Immunosorbent Techniques; D051379:Mice; D009857:Oncogenes; D011493:Protein Kinase C / Q000378:metabolism*; D051381:Rats
chemical_list: D000911:Antibodies, Monoclonal; D011493:Protein Kinase C