title: In vitro transcription of the nitrogen fixation regulatory operon nifLA of Klebsiella pneumoniae.
abstract: In vitro transcription from the promoter for the nitrogen fixation regulatory operon nifLA of K. pneumoniae requires four protein fractions: the core form of RNA polymerase; NTRA, an alternate sigma factor; NTRC, an auxiliary DNA-binding protein; and NTRB, a bifunctional enzyme that controls the activity of NTRC by covalent modification (A.J. Ninfa and B. Magasanik, Proc. Natl. Acad. Sci. USA 83:5909, 1986). Two DNA-binding sites for NTRC lie approximately 150 base pairs upstream of the nifLA promoter.
authors.forename: P K
authors.initials: PK
authors.lastname: Wong
authors.forename: D
authors.initials: D
authors.lastname: Popham
authors.forename: J
authors.initials: J
authors.lastname: Keener
authors.forename: S
authors.initials: S
authors.lastname: Kustu
mesh_terms: D001426:Bacterial Proteins; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics; D005786:Gene Expression Regulation; D005798:Genes, Bacterial; D066298:In Vitro Techniques; D007711:Klebsiella pneumoniae / Q000235:genetics*; D009586:Nitrogen Fixation; D009876:Operon; D051922:PII Nitrogen Regulatory Proteins; D011401:Promoter Regions, Genetic; D012808:Sigma Factor / Q000235:genetics; D015534:Trans-Activators; D014157:Transcription Factors / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; D051922:PII Nitrogen Regulatory Proteins; D012808:Sigma Factor; D015534:Trans-Activators; D014157:Transcription Factors