Cytoplasmic ribosomal proteins from Chlamydomonas reinhardtii: characterization and immunological comparisons.
Experiments were undertaken to characterize the cytoplasmic ribosomal proteins (r-proteins) in Chlamydomonas reinhardtii and to compare immunologically several cytoplasmic r-proteins with those of chloroplast ribosomes of this alga, Escherichia coli, and yeast. The large and small subunits of the C. reinhardtii cytoplasmic ribosomes were shown to contain, respectively, 48 and 45 r-proteins, with apparent molecular weights of 12,000-59,000. No cross-reactivity was seen between antisera made against cytoplasmic r-proteins of Chlamydomonas and chloroplast r-proteins, except in one case where an antiserum made against a large subunit r-protein cross-reacted with an r-protein of the small subunit of the chloroplast ribosome. Antisera made against one out of five small subunit r-proteins and three large subunit r-proteins recognized r-proteins from the yeast large subunit. Each of the yeast r-proteins has been previously identified as an rRNA binding protein. The antiserum to one large subunit r-protein cross-reacted with specific large subunit r-proteins from yeast and E. coli.
Fleming
G H
GH
Boynton
J E
JE
Gillham
N W
NW
D000906:Antibodies; D000936:Antigen-Antibody Complex; D002696:Chlamydomonas / Q000235:genetics*; D002736:Chloroplasts / Q000032:analysis; D003593:Cytoplasm / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D008970:Molecular Weight; D012269:Ribosomal Proteins / Q000235:genetics* / Q000302:isolation & purification; D012270:Ribosomes / Q000032:analysis; D012441:Saccharomyces cerevisiae / Q000235:genetics; D013045:Species Specificity
D000906:Antibodies; D000936:Antigen-Antibody Complex; D012269:Ribosomal Proteins