Differential behaviour of glucose 6-phosphate dehydrogenase in two morphological forms of Trypanosoma cruzi.
1. Glucose 6-phosphate dehydrogenase activity (EC 1.1.1.49) of two morphological forms of Trypanosoma cruzi, epimastigotes and metacyclics, are reported. 2. The kinetic behaviour and some of the kinetic parameters of the enzyme in both forms were studied. The enzymes showed a simple Michaelis-Menten kinetic. 3. The activity in epimastigote forms was alway higher than the metacyclic ones. At subsaturating concentrations of substrate was almost 10-fold higher, whereas at saturating concentrations was about 2-fold higher. 4. In epimastigote forms the specific activity and Km values, at pH 7.5 and 37 degrees C, was found to be 142 mUnits x mg-1 of protein and 0.23 mM, respectively. 5. In the same conditions, the specific activity and Km values in metacyclic forms was 75 mUnits x mg-1 of protein and 1.06 mM, respectively. 6. A possible role in the carbohydrate metabolism of glucose 6-phosphate dehydrogenase in both forms of Trypanosoma cruzi is discussed.
Lupiañez
J A
JA
Department of Biochemistry and Molecular Biology, Faculty of Science, University of Granada, Spain.
Adroher
F J
FJ
Vargas
A M
AM
Osuna
A
A
D000641:Ammonia / Q000378:metabolism; D000818:Animals; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism*; D007700:Kinetics; D014349:Trypanosoma cruzi / Q000201:enzymology* / Q000648:ultrastructure
D000641:Ammonia; D005954:Glucosephosphate Dehydrogenase