Immunocytochemical studies on the formation and repair of O6-alkylguanine in rat tissues.
The immunocytochemical visualization of the carcinogen-DNA adducts O6-ethylguanine (O6-etGua) and O6-methylguanine (O6-meGua) in histological sections of a large series of rat tissues, including liver, pancreas, testis and oesophagus, is described. In the liver of rats treated with N-ethyl-N-nitrosourea (ENU), N-nitrosodiethylamine (NDEA) or N-nitrosodimethylamine (NDMA), large differences were found in the formation and/or repair of O6-alkylGua between different cell types, between hepatocytes of different localization, and also between normal and precancerous hepatocytes. Heterogeneity of O6-etGua formation and repair was also found in pancreatic tissue of rats treated with ENU. O6-etGua was relatively persistent in nonparenchymal liver cells, pancreatic islet cells, spermatogonia, renal glomeruli and smooth-muscle cells. Our aim is to extend the immunocytochemical analysis to other DNA adducts, and ultimately to human tissues.
Scherer
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Netherlands Cancer Institute, Division of Chemical Carcinogenesis, Amsterdam.
Jenner
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den Engelse
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L
D000818:Animals; D004260:DNA Repair; D004052:Diethylnitrosamine / Q000494:pharmacology; D004128:Dimethylnitrosamine / Q000494:pharmacology; D005038:Ethylnitrosourea / Q000494:pharmacology; D006147:Guanine / Q000031:analogs & derivatives* / Q000378:metabolism; D007150:Immunohistochemistry; D007515:Islets of Langerhans / Q000187:drug effects / Q000378:metabolism; D007678:Kidney Glomerulus / Q000187:drug effects / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D009130:Muscle, Smooth / Q000187:drug effects / Q000378:metabolism; D011230:Precancerous Conditions / Q000235:genetics; D051381:Rats; D013093:Spermatogonia / Q000187:drug effects / Q000378:metabolism; D014018:Tissue Distribution
D004052:Diethylnitrosamine; D006147:Guanine; C008449:O-(6)-methylguanine; D004128:Dimethylnitrosamine; D005038:Ethylnitrosourea