[Duplication of a synthetic gene for human leukocyte interferon and its expression in polycistron mRNA with coupled translation system].
Using a chemically synthesised adapter, the coding part of an artificial gene for human leukocyte alpha 2 interferon (ifn-alpha 2) has been duplicated. The adapter contained a termination signal of the first gene (TAA) within the Shine-Dalgarno sequence of the second gene (TAAGGA), distance between the terminating codon and starting codon of the second gene being 11 nucleotides. In another case this distance was 69 nucleotides, with the same SD sequence. The expression of the tandems as a part of polycistrons has been studied under control of promoters Plac, (Ptrp)2 of E. coli, and PVIII of M13 phage. It was found that tandems of ifn-alpha 2 genes in polycistronic structures trp L-ifn-ifn and IX-VIII-ifn-ifn under control of promoters (Ptrp)2 and PVIII, respectively, provided high level of the interferon biosynthesis, thus differing from the tandem under Plac promoter control, which had only ifn-ifn translation coupling.
Kravchenko
V V
VV
Gileva
I P
IP
Shamin
V V
VV
Kulichkov
V A
VA
Dobrynin
V N
VN
D004926:Escherichia coli / Q000235:genetics; D005786:Gene Expression Regulation; D005796:Genes; D005813:Genes, Synthetic; D006801:Humans; D007370:Interferon Type I / Q000235:genetics*; D005810:Multigene Family; D010957:Plasmids; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics
D007370:Interferon Type I; D012333:RNA, Messenger
Duplikatsiia sinteticheskogo gena leĭkotsitarnogo interferona cheloveka i ego ékspressiia v sostave politsistronnykh mRNK s sopriazhennoĭ sistemoĭ transliatsii.