Recombinational substrates designed to study recombination between unique and repetitive sequences in vivo.
Three recombination events, reciprocal recombination, sister-chromatid recombination, and gene conversion, were studied using substrates designed in vitro. Each type of recombination event can be monitored at any chromosomal location. We have shown that sister-chromatid recombination is induced mitotically by DNA damaging agents, such as methyl methanesulfonate and gamma-rays, but is decreased mitotically in strains defective in rad52. Reciprocal recombination by which circular plasmids integrate into the genome is unaffected by rad52 defective alleles and occurs by a different recombination pathway. Mechanisms are suggested by which gene conversion between sister chromatids can generate chromosome rearrangements.
Fasullo
M T
MT
Davis
R W
RW
D000483:Alleles; D001483:Base Sequence; D004271:DNA, Fungal / Q000235:genetics; D005785:Gene Conversion; D010957:Plasmids; D011995:Recombination, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D012854:Sister Chromatid Exchange
D004271:DNA, Fungal