title: Monoclonal antibodies against human hematopoietic survival and growth factor.
abstract: Mouse hybridoma clones were isolated, and found to constitutionally secrete monoclonal antibody (MoAb) immunoglobulins specific for human hematopoietic stem cell growth factor (SCGF). MoAbs were initially screened by dot-blotting and enzyme immunoassay (EIA), and their specificity was confirmed using western blotting and EIA. Protein A-purified MoAb IgG neutralized SCGF activity, namely, granulocyte-macrophage colony potentiating and erythroid burst-promoting activities. The availability of MoAb could extend its application to one-step immunoadsorbent affinity-purification of SCGF as well as analysis on its receptor.
authors.affiliation: Department of Internal Medicine, Osaka Dental University, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Hiraoka
authors.forename: T
authors.initials: T
authors.lastname: Ohkubo
authors.forename: M
authors.initials: M
authors.lastname: Fukuda
mesh_terms: D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000302:isolation & purification*; D000918:Antibody Specificity; D002470:Cell Survival / Q000187:drug effects; D002850:Chromatography, Gel; D003101:Collodion; D004591:Electrophoresis, Polyacrylamide Gel; D006133:Growth Substances / Q000276:immunology*; D016298:Hematopoietic Cell Growth Factors; D006412:Hematopoietic Stem Cells / Q000187:drug effects*; D006801:Humans; D006825:Hybridomas / Q000378:metabolism; D007124:Immunoenzyme Techniques
chemical_list: D000911:Antibodies, Monoclonal; D006133:Growth Substances; D016298:Hematopoietic Cell Growth Factors; D003101:Collodion