Evidence for an inhibitory A1 subtype adenosine receptor on pancreatic insulin-secreting cells.
The effects of L- and D-phenylisopropyladenosine (L- and D-PIA) were studied on glucose-induced insulin secretion from the isolated perfused rat pancreas. L-PIA at the low dose of 16.5 nM inhibited insulin secretion by 50%. In contrast, D-PIA at 16.5 and 82.5 nM was ineffective. D-PIA used at a 100-fold higher concentration (1.65 microM) than L-PIA induced a similar inhibition of insulin secretion. The inhibitory effect of L-PIA was abolished by 8-phenyltheophylline (1 microM), a potent P1 purinoceptor antagonist. The present experiments provide evidence for an adenosine receptor of the A1 subtype on the insulin-secreting pancreatic cell of rats.
Hillaire-Buys
D
D
Bertrand
G
G
Gross
R
R
Loubatières-Mariani
M M
MM
D000818:Animals; D066298:In Vitro Techniques; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000166:cytology / Q000378:metabolism*; D010660:Phenylisopropyladenosine / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D011983:Receptors, Purinergic / Q000378:metabolism*; D013237:Stereoisomerism; D013806:Theophylline / Q000031:analogs & derivatives / Q000494:pharmacology
D007328:Insulin; D011983:Receptors, Purinergic; D010660:Phenylisopropyladenosine; D013806:Theophylline; C028322:8-phenyltheophylline