Immunonephelometry of apolipoprotein B with a centrifugal analyzer.
We developed an automated immunonephelometric assay for the measurement of apolipoprotein B (apo B) with a light-scattering microcentrifugal analyzer. Pretreating specimens with a dilute solution of Tween 20 or triglyceride lipase decreased the nephelometric response of apo B. Polyethylene glycol is included in the reaction mixture, and the reaction is complete within 4 min. The method is precise (CV = 6.5%, mean = 0.68 g/L) and the standard curve is linear to an apo B concentration of 2.8 g/L. Lipemia does not interfere with the method if grossly lipemic specimens are centrifuged to remove chylomicrons.
Hanson
N Q
NQ
Department of Laboratory Medicine and Pathology, University of Minnesota Medical School, Minneapolis.
LeGeault
T S
TS
Freier
E F
EF
D001055:Apolipoproteins B / Q000097:blood*; D001322:Autoanalysis / Q000379:methods; D006801:Humans; D007158:Immunologic Techniques; D008027:Light; D009391:Nephelometry and Turbidimetry / Q000379:methods; D012542:Scattering, Radiation; D014280:Triglycerides / Q000097:blood
D001055:Apolipoproteins B; D014280:Triglycerides