Surface proteins localized in ruffling membranes and filopodia of human glioma cells detected using Mab S-11E10.
We prepared mouse monoclonal antibody (Mab S-11E10) for the surface antigen specifically distributed in ruffling membranes and filopodia of cultured human glioma cells. The antibody reacted to the specified structures of other glioma cells (U251MG, KNS 60) as well as those of KNS 42 cells, which were the immunizing source. The antigens, identified by Mab S-11E10, had molecular weights of 65 and 66 kDa. Immunohistochemically, the antibody reacted only to astrocytes in the human brain, but the cross-reactivity was noted in extraneural tissues such as lymphocytes and epithelium of the bowel ducts. In 5 out of 6 low grade astrocytomas, most of the tumor cells were strongly stained, while tumor cells of highly cellular areas of anaplastic astrocytomas and glioblastomas were either not stained or only weakly stained. The specific localization of 65-66 kDa doublet proteins may suggest relation to spreading and locomotion of cells, and may correlate to astrocytic differentiation and/or function.
Sawa
H
H
Takeshita
I
I
Sato
Y
Y
Fukui
M
M
D000911:Antibodies, Monoclonal / Q000302:isolation & purification; D002460:Cell Line; D002462:Cell Membrane / Q000648:ultrastructure*; D002846:Chromatography, Affinity; D005455:Fluorescent Antibody Technique; D005910:Glioma; D006801:Humans; D008565:Membrane Proteins / Q000032:analysis*; D008854:Microscopy, Electron
D000911:Antibodies, Monoclonal; D008565:Membrane Proteins