Location and purification of enzymes on polyacrylamide gels using dialyzable fluorescent markers.
A method for the location of proteins/enzymes by polyacrylamide gel electrophoresis using dialyzable low-molecular-weight fluorescent peptide markers is described. The markers prepared by treating the peptic digest of casein with fluorescamine showed several bands on gel electrophoresis which helped in locating the proteins. The located desired protein could subsequently be purified by extraction.
Bodhe
A M
AM
Division of Biochemical Sciences, National Chemical Laboratory, Poona, India.
Vartak
H G
HG
Rele
M V
MV
Dhamankar
V S
VS
D019747:Aldose-Ketose Isomerases; D002238:Carbohydrate Epimerases / Q000302:isolation & purification*; D003956:Dialysis; D004591:Electrophoresis, Polyacrylamide Gel; D005450:Fluorescamine; D005453:Fluorescence; D005456:Fluorescent Dyes; D010940:Plant Proteins / Q000302:isolation & purification; D010944:Plants / Q000032:analysis; D012710:Serum Albumin, Bovine / Q000302:isolation & purification*; D013302:Streptomyces / Q000201:enzymology; D013381:Subtilisins / Q000037:antagonists & inhibitors*
D005456:Fluorescent Dyes; D010940:Plant Proteins; D012710:Serum Albumin, Bovine; D005450:Fluorescamine; D013381:Subtilisins; D002238:Carbohydrate Epimerases; D019747:Aldose-Ketose Isomerases; C023298:xylose isomerase