Comparison of wheat germ agglutinin- and phorbol myristate acetate-mediated triggering for macrophage H2O2 release: susceptibilities to various macrophage inhibitors.
Murine peritoneal macrophages (M phi S) elicited by a single injection of zymosan A showed a higher responsiveness to the wheat germ agglutinin (WGA)-mediated triggering for H2O2 release than M phi S activated by double injections of the agent. On the contrary, the response to phorbol myristate acetate(PMA)-mediated triggering was higher in the latter M phi S than in the case of the former M phi S. Furthermore, PMA-triggered M phi H2O2 release was found to be inhibited by sarcoma 180 tumor cell-derived proteinaceous factor in a much more marked fashion than the WGA-triggered H2O2 release. These results indicate some significant differences between the cellular mechanisms of the WGA- and PMA-triggering for M phi oxidative burst. On the other hand, microfilament-inhibitors (cytochalasins B and E) and serine protease-inhibitors (tosyl-L-lysine-chloromethyl ketone and tosylamido-2-phenylethyl-chloromethyl ketone), but not microtubule-disrupting agents (colchicine, vinblastine, and vincristine), suppressed both the WGA- and PMA-triggerings for M phi H2O2 release to a similar degree, thereby indicating that the WGA- and PMA-triggerings for M phi oxidative burst have a common process which is dependent on microfilament and serine protease functions. In relation to this, the WGA- and PMA-triggerings for M phi spreading were also depressed by microfilament- and serine protease-inhibitors but not by microtubule-inhibitors, indicating a participation of common membrane functions in the signal transduction in cases of M phi oxidative burst, and cell spreading induced by the WGA- as well as PMA-triggering.
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D008841:Actin Cytoskeleton / Q000187:drug effects; D000818:Animals; D003572:Cytochalasins / Q000494:pharmacology; D010450:Endopeptidases; D005260:Female; D006861:Hydrogen Peroxide / Q000378:metabolism*; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008809:Mice, Inbred C3H; D008870:Microtubules / Q000187:drug effects; D011480:Protease Inhibitors; D012697:Serine Endopeptidases; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D014909:Wheat Germ Agglutinins / Q000494:pharmacology*; D015054:Zymosan
D003572:Cytochalasins; D011480:Protease Inhibitors; D014909:Wheat Germ Agglutinins; D015054:Zymosan; D006861:Hydrogen Peroxide; D010450:Endopeptidases; D012697:Serine Endopeptidases; D013755:Tetradecanoylphorbol Acetate