title: Effect of cellular glutathione content on the induction of DNA double strand breaks by 25 MeV electrons.
abstract: The effect of endogenous glutathione (GSH) on the induction of DNA double strand breaks (dsb) by 25 MeV electrons was investigated using stationary haploid yeast cells defective in gamma-glutamyl-cysteine-synthetase (gsh 1) containing less than 5 per cent of the normal GSH content. In gsh 1 cells the induction of dsb is increased by a factor of 1.5 under oxic and 1.8 under anoxic irradiation conditions: whereas the oxygen enhancement ratio was only slightly decreased (1.9) compared to wild-type cells (2.4).
authors.forename: D
authors.initials: D
authors.lastname: Frankenberg
authors.forename: M
authors.initials: M
authors.lastname: Kistler
authors.forename: F
authors.initials: F
authors.lastname: Eckardt-Schupp
mesh_terms: D002470:Cell Survival / Q000528:radiation effects; D004247:DNA / Q000528:radiation effects*; D004271:DNA, Fungal / Q000528:radiation effects*; D004583:Electrons; D005978:Glutathione / Q000502:physiology*; D012441:Saccharomyces cerevisiae / Q000528:radiation effects*
chemical_list: D004271:DNA, Fungal; D004247:DNA; D005978:Glutathione