Engraftment following T-cell-depleted bone marrow transplantation. II. Stability of mixed chimerism in semiallogeneic recipients after total-body irradiation.
Chronic, stable mixed chimerism of both lymphocytes and erythrocytes was observed in semiallogeneic murine recipients of T-cell-depleted bone marrow transplants that had been conditioned with supralethal total-body irradiation (1100 cGy). Mixed chimerism was extensive, with a wide range of donor engraftment persisting for at least one year after transplant. In both erythrocyte and lymphocyte lineages, decreasing donor engraftment correlated with decreasing marrow dose; however, complete red cell engraftment was more easily achieved than complete lymphocyte engraftment. There were no late graft failures, even among animals exhibiting a substantial host component of hematopoiesis. The extent of mixed hematopoietic chimerism therefore appears to be much greater than had been expected in recipients of T-cell-depleted bone marrow transplants.
Ferrara
J L
JL
Department of Pediatrics, Harvard Medical School, Boston, Massachusetts.
Mauch
P
P
McIntyre
J
J
Michaelson
J
J
Burakoff
S J
SJ
D000818:Animals; D016026:Bone Marrow Transplantation; D004912:Erythrocytes; D006082:Graft Enhancement, Immunologic; D006085:Graft Survival; D006410:Hematopoiesis; D008212:Lymphocyte Depletion; D008214:Lymphocytes; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011828:Radiation Chimera