Mixed blood chimerism in T cell-depleted bone marrow transplant recipients: evaluation using DNA polymorphisms.
We have used DNA sequence polymorphism analysis to document engraftment after T cell-depleted bone marrow transplantation (BMT), with a selected panel of four DNA probes. In contrast to nondepleted BMT recipients, the patients who received T cell-depleted marrow exhibited a mixed blood chimerism. This mosaicism was observed before graft failure or relapse in six patients. However, in five other patients, this mixed chimerism was not followed by these complications with a follow-up of 9 to 31 months after transplantation. Our results support the hypothesis that transplanted bone marrow T cells may help to maintain engraftment by eliminating host cells that can cause graft failure.
Bretagne
S
S
INSERM U.91, Department of Genetics, Creteil, France.
Vidaud
M
M
Kuentz
M
M
Cordonnier
C
C
Henni
T
T
Vinci
G
G
Goossens
M
M
Vernant
J P
JP
D000328:Adult; D001402:B-Lymphocytes / Q000166:cytology; D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation; D002678:Chimera; D004273:DNA, Neoplasm / Q000235:genetics*; D005260:Female; D006098:Granulocytes / Q000166:cytology; D006801:Humans; D007938:Leukemia / Q000097:blood / Q000235:genetics / Q000628:therapy*; D008297:Male; D009693:Nucleic Acid Hybridization; D011110:Polymorphism, Genetic; D013601:T-Lymphocytes / Q000166:cytology*
D004273:DNA, Neoplasm