5-Fluoroorotic acid as a selective agent in yeast molecular genetics.
5-FOA is an extremely useful reagent for the selection of Ura- cells amid a population of Ura+ cells. The selection is effective in transformation and recombination studies where loss of URA3+ is desired. A new plasmid shuffling procedure based on the 5-FOAR selection permits the recovery of conditional lethal mutations in cloned genes that encode vital functions.
Boeke
J D
JD
Trueheart
J
J
Natsoulis
G
G
Fink
G R
GR
D000483:Alleles; D005800:Genes, Fungal; D005818:Genetic Engineering / Q000379:methods; D009154:Mutation; D009963:Orotic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development
D009963:Orotic Acid; C001242:5-fluoroorotic acid