Complementary DNA for human glioblastoma-derived T cell suppressor factor, a novel member of the transforming growth factor-beta gene family.
Human glioblastoma cells secrete a peptide, termed glioblastoma-derived T cell suppressor factor (G-TsF), which has suppressive effects on interleukin-2-dependent T cell growth. As shown here, complementary DNA for G-TsF reveals that G-TsF shares 71% amino acid homology with transforming growth factor-beta (TGF-beta). In analogy to TGF-beta it is apparently synthesized as the carboxy-terminal end of a precursor polypeptide which undergoes proteolytic cleavage to yield the 112 amino-acid-long mature form of G-TsF. Comparison of the amino-terminal sequence of G-TsF with that of porcine TGF-beta 2 and bovine cartilage-inducing factor B shows complete homology, which indicates that we have cloned the human analogue of these factors. It is tempting to consider a role for G-TsF in tumor growth where it may enhance tumor cell proliferation in an autocrine way and/or reduce immunosurveillance of tumor development.
de Martin
R
R
Sandoz Forschungsinstitut, Wien, Austria.
Haendler
B
B
Hofer-Warbinek
R
R
Gaugitsch
H
H
Wrann
M
M
Schlüsener
H
H
Seifert
J M
JM
Bodmer
S
S
Fontana
A
A
Hofer
E
E
D000595:Amino Acid Sequence; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism*; D005796:Genes; D005910:Glioma / Q000235:genetics* / Q000276:immunology; D006801:Humans; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000235:genetics*; D010455:Peptides / Q000235:genetics* / Q000276:immunology; D013491:Suppressor Factors, Immunologic / Q000235:genetics*; D013601:T-Lymphocytes / Q000276:immunology*; D016212:Transforming Growth Factor beta; D015291:Transforming Growth Factors
D009363:Neoplasm Proteins; D010455:Peptides; D013491:Suppressor Factors, Immunologic; D016212:Transforming Growth Factor beta; D015291:Transforming Growth Factors; D004247:DNA