title: The in vitro cultivation of P. falciparum ookinetes, and their enrichment on Nycodenz density gradients.
abstract: Employing a simple method of growing ookinetes of Plasmodium falciparum in culture, 40% of mature gametocytes convert to macrogametes, 4% reach the retort-form ookinete stage and 0.45% become mature ookinetes. A single-step gradient centrifugation method on 12.5% Nycodenz enriches both gametocytes and ookinetes.
authors.affiliation: Department of Pure and Applied Biology, Imperial College, London.
authors.forename: E H
authors.initials: EH
authors.lastname: Carter
authors.forename: A
authors.initials: A
authors.lastname: Suhrbier
authors.forename: P J
authors.initials: PJ
authors.lastname: Beckers
authors.forename: R E
authors.initials: RE
authors.lastname: Sinden
mesh_terms: D000818:Animals; D002499:Centrifugation, Density Gradient; D004912:Erythrocytes / Q000469:parasitology; D007472:Iohexol; D009024:Morphogenesis; D010963:Plasmodium falciparum / Q000166:cytology / Q000254:growth & development*
chemical_list: D007472:Iohexol