Vasoactive intestinal polypeptide (VIP) immunohistochemistry in the rabbit retina.
A commercially obtained antiserum to vasoactive intestinal polypeptide (VIP) was used with the avidin-biotin-peroxidase immunohistochemical method to localize VIP-like immunoreactivity in the adult rabbit retina. A population of cell bodies in the inner nuclear layer is specifically stained. The cells are most dense in the central retina (40-50 cells/mm2), and least numerous in the superior periphery (15-20 cells/mm2). The dendritic fields overlap extensively; cells in the periphery have dendritic fields up to 1 mm in diameter. The processes of the cells are in the inner plexiform layer, where they tend to form a tristratified pattern in sublayers 1, 3 and 5. This pattern of lamination is similar to that described by other laboratories for dopaminergic amacrine cells and provides further evidence for an interaction between these two neuromodulators.
Sagar
S M
SM
Neurology Service, Veterans Administration Medical Center, San Francisco, CA 94143.
D000818:Animals; D007124:Immunoenzyme Techniques; D008297:Male; D011817:Rabbits; D012160:Retina / Q000032:analysis* / Q000166:cytology; D014660:Vasoactive Intestinal Peptide / Q000032:analysis*
D014660:Vasoactive Intestinal Peptide