title: Estrogen receptor immunoreactivity in meningiomas. Comparison with the binding activity of estrogen, progesterone, and androgen receptors.
abstract: Estrogen receptor (ER) analysis was performed in 70 meningioma samples by means of two assays: an enzyme immunoassay that used monoclonal antibodies against human ER protein (estrophilin), and a sensitive radioligand binding assay that used iodine-125-labeled estradiol as the radioligand. Low levels of ER immunoreactivity were found in tumors from 51% of patients, whereas ER binding activity was demonstrated in 40% of the meningiomas examined. In eight (11%) of the tissue samples, multiple binding sites for estradiol were observed. The immunoreactive binding sites corresponded to those of the classic high-affinity ER. In ligand binding studies, however, measurement of classic ER was considerably influenced by a second low-affinity high-capacity estrogen binding component, even at low ligand concentrations. Binding activity of the progesterone receptor (PR) and androgen receptor (AR) was determined concurrently using 17 alpha-methyl-3H-promegestone (3H-R 5020) and 17 alpha-methyl-3H-trienolone (3H-R 1881), a synthetic gestagen and androgen, respectively. High concentrations of PR were detected in 53 (76%) of the tumors, whereas a moderate number of AR binding sites were demonstrated in 33 (47%) of the tumors. A positive correlation between ER immunoreactivity and AR binding activity is suggestive of estrogen regulation of AR via the ER system. The presence of gonadal steroid receptors in a large proportion of meningiomas and the tendency toward a dependence of receptor concentrations on the histological subtype of the meningioma could have implications for tumor therapy.
authors.forename: K P
authors.initials: KP
authors.lastname: Lesch
authors.forename: S
authors.initials: S
authors.lastname: Gross
mesh_terms: D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D008577:Meningeal Neoplasms / Q000276:immunology* / Q000378:metabolism; D008579:Meningioma / Q000276:immunology* / Q000378:metabolism; D008875:Middle Aged; D011869:Radioligand Assay; D011944:Receptors, Androgen / Q000378:metabolism; D011960:Receptors, Estrogen / Q000276:immunology* / Q000378:metabolism; D011980:Receptors, Progesterone / Q000378:metabolism
chemical_list: D011944:Receptors, Androgen; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone