Thyroid hormones regulate expression of the neural cell adhesion molecule in adult skeletal muscle.
Adult rat skeletal muscle does not express detectable levels of neural cell adhesion molecule (N-CAM) and membrane activity mediated by nerve appears to repress the N-CAM gene. N-CAM expression in skeletal muscle can however be reinduced by hypothyroidism. Thyroidectomised rats re-express N-CAM mRNAs of 5.2 and 2.9 kb and Western blot analysis showed protein bands of 125 and 155 kDa. Immunofluorescence analysis also showed high levels of N-CAM in the sarcolemma. These changes in N-CAM expression could be reversed by treatment of hypothyroid rats with thyroxine. Thus thyroid hormones as well as neural influences appear to control N-CAM expression in skeletal muscle.
Thompson
J
J
Moore
S E
SE
Walsh
F S
FS
D000818:Animals; D000954:Antigens, Surface / Q000096:biosynthesis* / Q000235:genetics; D015815:Cell Adhesion Molecules; D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation; D006801:Humans; D007037:Hypothyroidism / Q000378:metabolism; D008297:Male; D009132:Muscles / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012508:Sarcolemma / Q000378:metabolism; D013963:Thyroid Hormones / Q000502:physiology*; D013965:Thyroidectomy
D000954:Antigens, Surface; D015815:Cell Adhesion Molecules; D013963:Thyroid Hormones