Molecular weight of recombinant human tumor necrosis factor-alpha.
Recombinant DNA-derived human tumor necrosis factor-alpha from Escherichia coli was examined by equilibrium ultracentrifugation under conditions similar to those where gel filtration experiments suggested an oligomeric structure. Short-column equilibrium experiments at concentrations in the range 0.015-0.12% at pH 8.5 in 0.04 M Tris/Tris-HCl gave molecular weights corresponding to 3 times the sequence molecular weight both in the presence and absence of 0.1 M NaCl. Long (2.6 mm)-column experiments under the same solvent conditions indicated molecular weights of 51,900 +/- 900 in the absence of added NaCl and 52,600 +/- 700 in the presence of added 0.1 M NaCl. No evidence of any species other than the trimer was found.
Arakawa
T
T
Yphantis
D A
DA
D004926:Escherichia coli / Q000235:genetics; D006023:Glycoproteins / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D008233:Lymphotoxin-alpha; D008970:Molecular Weight; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha
D006023:Glycoproteins; D008233:Lymphotoxin-alpha; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha