Serogrouping single colonies of beta-hemolytic streptococci with achromopeptidase extraction.
Achromopeptidase (TBL-1; Wako Chemicals, USA, Inc., Dallas, Tex.) prepared as a 2,000-U/ml solution will extract the serogroup antigens from single colonies of groups A, B, C, F, and G streptococci in 1 min at room temperature. This enzyme extraction is not effective for the serogrouping of all group D streptococcus species. Achromopeptidase extracts can be used with latex or coagglutination reagents.
Slifkin
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Cumbie
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D000372:Agglutination Tests; D000942:Antigens, Bacterial / Q000032:analysis* / Q000302:isolation & purification; D001433:Bacteriolysis; D010450:Endopeptidases; D012697:Serine Endopeptidases; D012703:Serotyping; D013291:Streptococcus / Q000145:classification* / Q000276:immunology
D000942:Antigens, Bacterial; D010450:Endopeptidases; D012697:Serine Endopeptidases; C036813:lysyl endopeptidase