Proteolysis by toxigenic Aspergillus nidulans from Nigerian palm produce.
The submerged cultures of Aspergillus nidulans had optimal growth and protease production at 37 degrees C and within 6 days of incubation. A rapid drop in pH of the growth medium from 6.9 to 4.8 and a subsequent gradual rise was recorded with the period of incubation. The acid-protease produced was purified by a combination of ethanolic precipitation, ultrafiltration and fractionation on DEAE-cellulose and Sephadex G-200. A single peak showing protease activity was subsequently obtained with a 16-fold increase in specific activity and a recovery value of 36%. The purified enzyme had optimal activity on casein and gelatin at pH 5.4 and a temperature of 40 degrees C.
Ogundero
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D001233:Aspergillus nidulans / Q000201:enzymology* / Q000254:growth & development; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D010450:Endopeptidases / Q000032:analysis / Q000302:isolation & purification; D005516:Food Microbiology; D006863:Hydrogen-Ion Concentration; D009549:Nigeria; D010447:Peptide Hydrolases / Q000302:isolation & purification* / Q000378:metabolism; D013696:Temperature
D010450:Endopeptidases; D010447:Peptide Hydrolases