title: Inactivation of the proteolytic activity of mouse nerve growth factor by human C1(activated)-inhibitor.
abstract: The interaction between the serine protease gamma subunit of NGF (gamma-NGF) and human C1(activated)-inhibitor (C1-Inh) has been studied. C1-Inh inactivates the protease activity of gamma-NGF as measured by its ability to cleave the synthetic substrate benzoyl-arginine-p-nitroanilide (L-BAPNA). Experiments in which gamma-NGF and C1-Inh were mixed at differing molar ratios indicated that inhibition was due to the formation of a 1:1 stoichiometric complex. Analysis of the interaction of 125I-labeled gamma-NGF with C1-Inh by SDS-PAGE and autoradiography indicated that a covalent bond was formed between gamma-NGF and C1-Inh. The covalent bond was hydrolyzed by hydroxylamine, which suggested that the two proteins were linked via an acyl linkage. The formation of this complex was time dependent and required the proteolytic activity of the gamma-NGF.
authors.forename: E L
authors.initials: EL
authors.lastname: Faulmann
authors.forename: M
authors.initials: M
authors.lastname: Young
authors.forename: M D
authors.initials: MD
authors.lastname: Boyle
mesh_terms: D000818:Animals; D003174:Complement C1 Inactivator Proteins / Q000378:metabolism / Q000494:pharmacology*; D010450:Endopeptidases / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects; D006801:Humans; D051379:Mice; D009414:Nerve Growth Factors / Q000037:antagonists & inhibitors* / Q000378:metabolism; D011480:Protease Inhibitors; D011485:Protein Binding; D012697:Serine Endopeptidases; D013997:Time Factors
chemical_list: D003174:Complement C1 Inactivator Proteins; D009414:Nerve Growth Factors; D011480:Protease Inhibitors; D010450:Endopeptidases; D012697:Serine Endopeptidases