An immunological assay for chloramphenicol acetyltransferase.
We report the production and utility of rabbit polyclonal antisera to the bacterial protein chloramphenicol acetyltransferase (CAT). The anti-CAT antibodies specifically react with CAT protein produced in bacterial or avian cells. Results reported here demonstrate that detection of CAT by immuno-dot blot techniques is at least as sensitive as the currently used in vitro enzymatic assay. The availability of antibodies to CAT offers a number of potential advantages to investigators who now use the CAT gene to study factors influencing gene regulation.
Burns
D K
DK
Crowl
R M
RM
D000123:Acetyltransferases / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D000818:Animals; D000918:Antibody Specificity; D015500:Chloramphenicol O-Acetyltransferase; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D005798:Genes, Bacterial; D007118:Immunoassay / Q000379:methods; D010957:Plasmids; D011784:Quail
D000123:Acetyltransferases; D015500:Chloramphenicol O-Acetyltransferase