Expression of restricted immunoglobulin isotypes in plasmacellular hyperplasia after allogeneic bone marrow transplantation.
Plasmacellular hyperplasia in lymphoid tissue was found in 4 out of 9 patients 1-6 months after allogeneic bone marrow transplantation as treatment for leukaemia. In the plasma cell populations, 13-85% expressed a single immunoglobulin light and heavy chain isotype (monotypic Ig expression). DNA analysis, using a DNA probe specific for heavy chain JH gene segments and for light chains, did not reveal the presence of clonally restricted B lymphocytes. The patients' sera lacked homogeneous immunoglobulins. We conclude that plasmacellular hyperplasia found after allogeneic bone marrow transplantation represents a polyclonal B-cell expansion- with a restriction in Ig isotype.
Schuurman
H J
HJ
Department of Internal Medicine, University Hospital, Utrecht, The Netherlands.
Hendriks
R W
RW
Verdonck
L F
LF
van Baarlen
J
J
de Gast
G C
GC
Schuurman
R K
RK
D001402:B-Lymphocytes / Q000276:immunology; D016026:Bone Marrow Transplantation; D004247:DNA / Q000032:analysis; D006087:Graft vs Host Reaction; D006801:Humans; D006965:Hyperplasia; D007132:Immunoglobulin Isotypes / Q000032:analysis*; D008198:Lymph Nodes / Q000473:pathology; D008221:Lymphoid Tissue / Q000473:pathology*; D008223:Lymphoma / Q000473:pathology; D010950:Plasma Cells / Q000473:pathology*; D014184:Transplantation, Homologous
D007132:Immunoglobulin Isotypes; D004247:DNA