Beta-actinin is not distinguishable from an actin barbed-end capping protein in chicken breast muscle.
beta-Actinin is an actin-pointed end capping protein in skeletal muscle. Casella et al. have reported that a protein isolated from muscle acetone powder by procedures similar to those used for beta-actinin purification caps the barbed end of an actin filament (J. Biol. Chem. 261, 10915-10921 (1986)). We have confirmed the above results. However, it turned out that the two proteins were identical as to subunit sizes, peptide maps, and cross-reactivities with anti-beta-actinin IgG. The binding of the two proteins to opposite ends of an actin filament remains unexplained.
Oosawa
M
M
Department of Biology, Faculty of Science, Chiba University.
Shimaoka
S
S
Funatsu
T
T
Ishiwata
S
S
Maruyama
K
K
D051339:Actin Depolymerizing Factors; D000199:Actins / Q000302:isolation & purification; D000818:Animals; D002645:Chickens; D051345:Destrin; D004591:Electrophoresis, Polyacrylamide Gel; D007158:Immunologic Techniques; D007525:Isoelectric Focusing; D008840:Microfilament Proteins / Q000302:isolation & purification; D009132:Muscles / Q000032:analysis*
D051339:Actin Depolymerizing Factors; D000199:Actins; D051345:Destrin; D008840:Microfilament Proteins