Monoclonal antibody GB36 raised against human trophoblast recognizes a novel epithelial antigen.
Monoclonal antibody GB36, which was raised against human term syncytiotrophoblastic microvilli, was found to recognize a novel epithelial antigen. The antibody immunoprecipitated several membrane proteins from BeWo (choriocarcinoma) and HT-29 (colon adenocarcinoma) cells. Under non-reducing conditions, four peptides of 180, 155, 135 and 130 kDa were revealed by SDS-PAGE analysis. Three peptides of slightly higher molecular weights were revealed under reducing conditions; these three peptides had identical pI (6.2) as shown by two-dimensional gel electrophoresis. By immunofluorescence, GB36 reacted with villous cytotrophoblasts and cytotrophoblasts of chorion laeve, as well as with the basal surface of syncytiotrophoblast and amniotic epithelium. Extravillous trophoblasts of cytotrophoblastic shell in the basal plate and the cytotrophoblastic cell island were non-reactive. It is suggested that the antigen of GB36 may play a role in the polarity of epithelial cells and the adhesion of epithelial cells to extracellular matrix.
Hsi
B L
BL
Yeh
C J
CJ
Samson
M
M
Fehlmann
M
M
D000911:Antibodies, Monoclonal; D000941:Antigens / Q000032:analysis*; D001485:Basement Membrane / Q000032:analysis; D004848:Epithelium / Q000032:analysis / Q000276:immunology; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007525:Isoelectric Focusing; D008856:Microscopy, Fluorescence; D008970:Molecular Weight; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D011263:Pregnancy Trimester, Third; D014327:Trophoblasts / Q000166:cytology / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000941:Antigens