Crystals of seryl-tRNA synthetase from Escherichia coli. Preliminary crystallographic data.
Crystals of seryl-tRNA synthetase from Escherichia coli can be grown from ammonium sulphate/octyl glucoside solutions in two days. The crystals appear to be very suitable for X-ray analysis, diffracting to at least 2.8 A resolution and being resistant to radiation damage. The crystals are monoclinic (space group C2) with cell parameters a = 148.2 A, b = 90.6 A, c = 69.5 A and beta = 119.0 degrees. Depending on whether the asymmetric unit is the enzyme monomer (Mr 48,414) or dimer the Vm value would be either 4.12 or 2.10 A3/dalton. Although the former would indicate a rather high solvent content, other proteins crystallized in the presence of octyl glucoside have Vm values similar to this.
Leberman
R
R
Berthet-Colominas
C
C
Cusack
S
S
Härtlein
M
M
D000604:Amino Acyl-tRNA Synthetases; D003461:Crystallography; D004926:Escherichia coli / Q000201:enzymology*; D012714:Serine-tRNA Ligase
D000604:Amino Acyl-tRNA Synthetases; D012714:Serine-tRNA Ligase