CD5-positive B cells after T cell depleted bone marrow transplantation.
CD5 is an antigen normally found on T cells and on a minority subpopulation of B cells in fetal spleen and tonsil and on the majority of cells in B-chronic lymphocytic leukaemia. Recent reports described the occurrence of large numbers of CD5-positive B cells in the peripheral blood after bone marrow transplantation (BMT). We examined the peripheral blood for CD5-positive B cells in 21 patients who underwent allogeneic BMT for leukaemia with marrow first depleted of T cells using anti-T monoclonal antibodies and complement mediated lysis. Twenty-six samples were obtained from patients 24-644 days after BMT and examined for the existence of blood-borne CD5-positive B cells by immunofluorescence analysis microscopically and by flow cytometry. The number of CD5-positive B cells was consistently lower than 2%. The absence of CD5-positive B cells in this series may be due to the method of T cell depletion of the marrow or to methodological differences in the analysis of the cells.
Drexler
H G
HG
Royal Free Hospital, Department of Haematology, London, UK.
Brenner
M K
MK
Wimperis
J Z
JZ
Gignac
S M
SM
Janossy
G
G
Prentice
H G
HG
Hoffbrand
A V
AV
D000293:Adolescent; D000328:Adult; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology*; D016026:Bone Marrow Transplantation; D002648:Child; D005260:Female; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006801:Humans; D007938:Leukemia / Q000628:therapy; D007945:Leukemia, Lymphoid / Q000276:immunology; D007958:Leukocyte Count; D008212:Lymphocyte Depletion; D008297:Male; D008875:Middle Aged; D013601:T-Lymphocytes / Q000276:immunology*
D000945:Antigens, Differentiation, T-Lymphocyte