title: Eucaryotic primase.
abstract: Eucaryotic primase, an enzyme that initiates de novo DNA replication, is tightly associated with polymerase alpha or yeast DNA polymerase I. It is probably a heterodimer of 5.6 +/- 0.1 S. The enzyme synthesizes oligoribonucleotides of about eight residues which are always initiated with a purine. In vitro the polymerase-primase complex initiates synthesis and pauses at preferred sites on natural single-stranded templates. The relative concentrations of ATP and GTP present in the reaction medium modulate the frequency of site recognition. Primase is strongly ATP-dependent in the presence of single-stranded DNA and of poly(dT). It also synthesizes oligo(rG) in the presence of poly(dC) very efficiently.
authors.forename: Y F
authors.initials: YF
authors.lastname: Roth
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000201:enzymology; D002477:Cells / Q000201:enzymology*; D019915:DNA Primase; D004261:DNA Replication; D005057:Eukaryotic Cells / Q000201:enzymology*; D005419:Flavonoids; D006801:Humans; D010636:Phenols / Q000378:metabolism; D011071:Poly T / Q000378:metabolism; D011108:Polymers / Q000378:metabolism; D059808:Polyphenols; D012316:RNA Nucleotidyltransferases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D013698:Templates, Genetic
chemical_list: D005419:Flavonoids; D010636:Phenols; D011108:Polymers; D059808:Polyphenols; D011071:Poly T; D019915:DNA Primase; D012316:RNA Nucleotidyltransferases