Heme increases peroxidase-antibody activity in aged conjugates.
Decay of activity in antibody-peroxidase conjugates is faster when highly diluted as in clinical kits. Following aging at 4 degrees C, some of this loss of activity can be restored by incubation with heme just before use. The addition of heme in the conjugate storage buffer prevents some decay of activity and is not due to the pseudoperoxidase activity of heme. This procedure permits conjugates to be presented in an easy ready-to-use form for clinical immunoenzymatic assays.
Coll
J M
JM
Laboratorio de Investigación, INVESGEN, Madrid, Spain.
D000906:Antibodies; D002097:C-Reactive Protein / Q000276:immunology; D006418:Heme; D006735:Horseradish Peroxidase; D007124:Immunoenzyme Techniques; D010544:Peroxidases; D011309:Preservation, Biological; D013997:Time Factors
D000906:Antibodies; D006418:Heme; D002097:C-Reactive Protein; D006735:Horseradish Peroxidase; D010544:Peroxidases