A specific enzyme-linked immunosorbent assay for definition of the IgG antibody response to disulphide-conjugated D-penicillamine in the rabbit.
An enzyme-linked immunosorbent assay (ELISA) has been developed for unambiguous detection of antibodies against the sulphydryl drug D-penicillamine (PA) and its disulphide-conjugated metabolites. Disulphide-linked PA human serum albumin (PA-HSA) conjugates for use as coating antigens were prepared by a range of procedures employing oxidation with either potassium ferricyanide (0.1 M) or cupric sulphate (5 ppm). A satisfactory degree of conjugation was achieved by both oxidative procedures. Hapten density and antigenicity were increased when urea-denatured rather than non-denatured HSA was used. In 2 out of 3 rabbits, a specific IgG anti-PA response was detected following monthly injection of PA keyhole limpet haemocyanin (PA-KLH) in Freund's complete adjuvant. In the third rabbit, any anti-PA activity was obscured by a high level of binding to HSA. The anti-PA response was slow to develop in the 2 responder rabbits (requiring four injections) and was of low intensity (antibody titres less than 6,000). In contrast, the IgG antibody response to the structurally related drug captopril (CP), administered under identical conditions, was rapid in onset and of greater intensity (titres greater than 6,000 after one injection of CP-KLH). The hapten specificity of the IgG anti-PA-HSA antisera was defined by ELISA inhibition assays. Binding of IgG to PA-HSA was inhibited by PA, PA disulphide, PA cysteine and disulphide-linked PA-HSA conjugates, but not by PA acetone (thiazolidine ring-linked PA), CP, or unconjugated HSA. The inhibitory preparations were inactive in unrelated ELISAs.(ABSTRACT TRUNCATED AT 250 WORDS)
Foster
A L
AL
Department of Pharmacology and Therapeutics, University of Liverpool, UK.
Park
B K
BK
Coleman
J W
JW
D000818:Animals; D000918:Antibody Specificity; D002216:Captopril / Q000276:immunology; D004220:Disulfides; D004797:Enzyme-Linked Immunosorbent Assay; D006241:Haptens / Q000276:immunology; D007074:Immunoglobulin G / Q000032:analysis*; D008297:Male; D010396:Penicillamine / Q000276:immunology*; D011817:Rabbits; D012709:Serum Albumin / Q000276:immunology
D004220:Disulfides; D006241:Haptens; D007074:Immunoglobulin G; D012709:Serum Albumin; D002216:Captopril; D010396:Penicillamine