title: A comparison of spleen and lymph node cells as fusion partners for the raising of monoclonal antibodies after different routes of immunisation.
abstract: Administration of antigen (human insulin) via the rear footpads of BALB/c mice with subsequent fusion of popliteal and inguinal lymph node lymphocytes induced a higher frequency of hybridomas (100%) secreting specific antibody than either intradermal immunisation and lymph node cell fusion (53%) or conventional subcutaneous immunisation and intraperitoneal boost followed by splenic lymphocyte fusion (8%). The rank order of serum antibody titres was found to correlate with the order of fusion efficiencies. Lymph node cell fusions also produced a greater spectrum of antibody specificities. Such differences in fusion efficiencies were also observed using bovine intestinal alkaline phosphatase.
authors.affiliation: Department of Medicine II, General Hospital, Southampton, U.K.
authors.forename: I H
authors.initials: IH
authors.lastname: Mirza
authors.forename: T J
authors.initials: TJ
authors.lastname: Wilkin
authors.forename: M
authors.initials: M
authors.lastname: Cantarini
authors.forename: K
authors.initials: K
authors.lastname: Moore
mesh_terms: D000469:Alkaline Phosphatase / Q000276:immunology; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D002459:Cell Fusion; D005260:Female; D006825:Hybridomas / Q000166:cytology*; D007114:Immunization / Q000379:methods*; D007328:Insulin / Q000276:immunology; D008198:Lymph Nodes / Q000166:cytology*; D051379:Mice; D013154:Spleen / Q000166:cytology*
chemical_list: D000911:Antibodies, Monoclonal; D007328:Insulin; D000469:Alkaline Phosphatase