The effect of secretin on the glucose-induced insulin release from isolated pancreatic islets in mice.
A simple and rapid method for isolation of pancreatic islets from mice using Percoll as a separation medium is described. Increasing concentrations of secretin from 10(-11) to 10(-6) M were without effect on the insulin release from the islets at 5 X 5 mM glucose, whereas significant increases were found at 12 mM glucose. Secretin did not elicit additional increases of the insulin release at 5 X 5 and 12 mM glucose with 5 mM theophylline. The mechanism by which secretin acts on the pancreatic islets is discussed. Based upon the above observations it is suggested that secretin apparently represents a modulator of the insulin release from the pancreatic islets.
Florholmen
J
J
Vonen
B
B
Giaever
A K
AK
Burhol
P G
PG
D000818:Animals; D002469:Cell Separation; D005947:Glucose / Q000494:pharmacology*; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D012633:Secretin / Q000494:pharmacology*; D013806:Theophylline / Q000494:pharmacology
D007328:Insulin; D012633:Secretin; D013806:Theophylline; D005947:Glucose