Production and characterization of monoclonal antibodies to 17 alpha-hydroxyprogesterone.
Hybridoma clones producing antibodies to 17 alpha-hydroxyprogesterone (17-OHP) were established by using a 17-OHP-bovine serum albumin conjugate as an immunogen. Six representative IgG-class monoclonal antibodies of high affinity (10(8)-10(9) M-1) showed differential reactivities with several structurally related steroids. Two enzyme immunoassay (EIA) systems (fluorescence EIA and micro-EIA) for 17-OHP using OHP 4B2.2.3, which showed the lowest cross-reactivity with other steroids, were established. The micro-EIA system was shown to be applicable to the mass-screening of congenital adrenal hyperplasia.
Sawada
J
J
Division of Radiochemistry, National Institute of Hygienic Sciences, Tokyo, Japan.
Terao
T
T
Itoh
S
S
Maeda
M
M
Tsuji
A
A
Hosoda
H
H
Nambara
T
T
D019326:17-alpha-Hydroxyprogesterone; D000312:Adrenal Hyperplasia, Congenital / Q000097:blood; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D000941:Antigens / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006825:Hybridomas / Q000276:immunology; D006908:Hydroxyprogesterones / Q000097:blood / Q000276:immunology*; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000276:immunology; D008403:Mass Screening; D051379:Mice; D008807:Mice, Inbred BALB C; D012710:Serum Albumin, Bovine / Q000276:immunology
D000911:Antibodies, Monoclonal; D000941:Antigens; D006908:Hydroxyprogesterones; D007074:Immunoglobulin G; D012710:Serum Albumin, Bovine; D019326:17-alpha-Hydroxyprogesterone