A 2D-1H-n.m.r. study of some Shigella flexneri O-polysaccharides.
A 1H-n.m.r. study of the O-polysaccharides from different types of Shigella flexneri has been performed. With the aid of 2D-n.m.r. techniques, namely, J-resolved, spin-spin correlation, and NOESY experiments, most of the structural features of these polysaccharides could be deduced. Sequences could generally be obtained from the NOESY experiments. When using a prolonged mixing time in these experiments, cross-peaks due to spin diffusion from one anomeric proton to the anomeric proton of an adjacent residue could be obtained, thereby giving complementary sequence information.
Jansson
P E
PE
Department of Organic Chemistry, Arrhenius Laboratory, University of Stockholm, Sweden.
Kenne
L
L
Wehler
T
T
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008969:Molecular Sequence Data; D011135:Polysaccharides, Bacterial; D012763:Shigella flexneri / Q000276:immunology*
D011135:Polysaccharides, Bacterial