title: The role of interleukin 1 and 2 in generation of acquired resistance against mouse typhoid infection afforded by dialyzable factor from Salmonella typhimurium.
abstract: Dialyzable factor (DF) prepared from a ribosomal fraction of Salmonella typhimurium was tested for its ability to induce interleukin 1 (IL 1) and 2 (IL 2) production, in relation to acquired resistance, after an intraperitoneal injection of DF. IL 1 production in vitro by peritoneal macrophages of DF-treated mice reached the maximum 4 days after injection, at the time when the nonspecific local resistance via macrophages directly activated with DF became apparent (Kita et al, Microbiol. Immunol. 28:807, 1984). Concanavalin A-induced IL 2 production by splenocytes of DF-treated mice reached the maximal level between days 6 and 8, and it could be enhanced even on day 14. Antigen-induced blastogenic responses of splenocytes from DF-treated mice reached the maximal level 14 days after treatment. Although DF did not show the mitogenic activity to normal splenocytes, T cells of DF-treated mice could respond to S. typhimurium. On the contrary, T cells of normal mice could respond to heat-killed cells of S. typhimurium when they were cultured with macrophages which had been directly stimulated in vitro with DF. Furthermore, T cells from DF-treated mice could respond to antigens of different species of bacteria, and especially to Listeria monocytogenes. These results suggest that T cells of DF-treated mice, being at the intermediate stage of activation via monokines including IL 1 which is produced by macrophages stimulated with DF, are able to proliferate immediately after the administration of challenging organisms as a second signal, and also that the specificity of the response may be defined by the challenging organisms.
authors.affiliation: Department of Bacteriology, Nara Medical College.
authors.forename: E
authors.initials: E
authors.lastname: Kita
authors.forename: M
authors.initials: M
authors.lastname: Emoto
authors.forename: K
authors.initials: K
authors.lastname: Nishi
authors.forename: N
authors.initials: N
authors.lastname: Katsui
authors.forename: S
authors.initials: S
authors.lastname: Kashiba
mesh_terms: D000818:Animals; D005260:Female; D066298:In Vitro Techniques; D007375:Interleukin-1 / Q000096:biosynthesis*; D007376:Interleukin-2 / Q000096:biosynthesis*; D007700:Kinetics; D008213:Lymphocyte Activation; D008262:Macrophage Activation; D051379:Mice; D012486:Salmonella typhimurium / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology; D014435:Typhoid Fever / Q000276:immunology*
chemical_list: D007375:Interleukin-1; D007376:Interleukin-2