title: PUVA-induced blisters, complement deposition, and damage to the dermoepidermal junction.
abstract: We followed the course of 56 patients receiving psoralen plus long-wave ultraviolet light (PUVA) therapy. Nonhemorrhagic blisters developed on clinically normal skin on the limbs of seven patients. Seeming to be related to friction and trauma, the blisters form as a result of damage to the basal and suprabasal layers. Perilesional skin specimens from all blistered patients contained granular deposits of C3 at the dermoepidermal junction, around the upper dermal blood vessels, or at both sites. The average time for initiation and complete formation of suction blisters was measured in 51 patients at different stages during the course of PUVA treatment. Blister separation was in the lamina lucida, with the pemphigoid antigen in the roof while the blister floor contained the lamina densa, laminin, and type IV collagen. This impaired dermoepidermal adhesion was a general phenomenon that occurred in all PUVA-treated patients. The mechanism remains to be determined.
authors.affiliation: Department of Dermatology, University of Newcastle-Upon-Tyne, England.
authors.forename: P S
authors.initials: PS
authors.lastname: Friedmann
authors.forename: P
authors.initials: P
authors.lastname: Coburn
authors.forename: M G
authors.initials: MG
authors.lastname: Dahl
authors.forename: B L
authors.initials: BL
authors.lastname: Diffey
authors.forename: J
authors.initials: J
authors.lastname: Ross
authors.forename: G P
authors.initials: GP
authors.lastname: Ford
authors.forename: S C
authors.initials: SC
authors.lastname: Parker
authors.forename: P
authors.initials: P
authors.lastname: Bird
mesh_terms: D000328:Adult; D001706:Biopsy; D001768:Blister / Q000209:etiology* / Q000473:pathology; D003176:Complement C3 / Q000276:immunology*; D004817:Epidermis / Q000187:drug effects; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D008297:Male; D011701:PUVA Therapy / Q000009:adverse effects*; D012867:Skin / Q000276:immunology* / Q000473:pathology; D013396:Suction
chemical_list: D003176:Complement C3