title: A ribosome-associated inhibitor of in vitro nonsense suppression in [psi-] strains of yeast.
abstract: All classes of tRNA-mediated nonsense suppression are much more efficient in yeast cell-free lysates prepared from a [psi+] strain than in those prepared from an isogenic [psi-] strain. Mixed [psi+]/[psi-] lysates do not support efficient suppression. Fractionation of the [psi-] lysate demonstrated the presence of an inhibitor of in vitro suppression that is loosely associated with the 80 S ribosome. The data indicate that the inhibitor is a factor involved in the termination of translation in this simple eukaryote.
authors.affiliation: Biological Laboratory, University of Kent, Canterbury, England.
authors.forename: M F
authors.initials: MF
authors.lastname: Tuite
authors.forename: B S
authors.initials: BS
authors.lastname: Cox
authors.forename: C S
authors.initials: CS
authors.lastname: McLaughlin
mesh_terms: D014176:Protein Biosynthesis; D012331:RNA, Fungal / Q000235:genetics; D012343:RNA, Transfer / Q000235:genetics; D012269:Ribosomal Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D012270:Ribosomes / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D013489:Suppression, Genetic / Q000187:drug effects*
chemical_list: D012331:RNA, Fungal; D012269:Ribosomal Proteins; D012343:RNA, Transfer