title: Phosphorylation of ribosomal protein S6 during lens cell differentiation: correlation with translational efficiency.
abstract: In vitro differentiation of embryonic chicken lens epithelial explants to form lens fiber cells is accompanied by an increase in protein synthesis without a corresponding increase in mRNA levels. This apparent increase in translational efficiency is correlated with a specific enhancement of phosphorylation of a 32K protein, which we identify as ribosomal protein S6 by two dimensional gel electrophoresis of purified ribosomal proteins. Serum, insulin, and chicken vitreous humor, three agents known to initiate differentiation in this system, all lead to enhanced S6 phosphorylation. Maximal enhancement of phosphorylation is reached within the first hour after the onset of differentiation, and is not blocked by inhibitors of RNA and protein synthesis.
authors.forename: N D
authors.initials: ND
authors.lastname: Vu
authors.forename: P
authors.initials: P
authors.lastname: Zelenka
mesh_terms: D000818:Animals; D001790:Blood Physiological Phenomena; D002417:Cattle; D002454:Cell Differentiation; D002642:Chick Embryo; D003459:Crystallins / Q000378:metabolism*; D004586:Electrophoresis; D007328:Insulin / Q000494:pharmacology; D007908:Lens, Crystalline / Q000166:cytology* / Q000196:embryology; D010766:Phosphorylation; D014176:Protein Biosynthesis; D012269:Ribosomal Proteins / Q000378:metabolism*; D014822:Vitreous Body / Q000502:physiology
chemical_list: D003459:Crystallins; D007328:Insulin; D012269:Ribosomal Proteins