[Demonstration of IgE associated cells in atopic nasal mucosa using the immunoperoxidase method].
IgE-associated cells in the specimens of 25 allergic and non-allergic subjects were stained by the peroxidase technique with the use of a polyclonal antiserum. The technique is described and its advantages over the immunofluorescence procedures are shown. In each allergic subject IgE-associated cells could be stained, whereas no cells could be found in non-atopic subjects after suppressing non-specific background staining (Fig. 4). Therefore, this method allows to distinguish between IgE-mediated allergic diseases of the nasal mucosa and diseases of other pathomechanisms. The stained cells formed a specific distribution pattern with a typical accumulation in the subepithelial and epithelial layer (Fig. 2), whereas only few cells were found in the mucosal glands.
Bachert
C
C
Hals-, Nasen-, Ohrenklinik, Klinikum Mannheim der Universität Heidelberg.
Ganzer
U
U
D004804:Eosinophils / Q000473:pathology; D004848:Epithelium / Q000473:pathology; D006801:Humans; D007124:Immunoenzyme Techniques; D007073:Immunoglobulin E / Q000378:metabolism*; D009297:Nasal Mucosa / Q000473:pathology*; D012221:Rhinitis, Allergic, Perennial / Q000473:pathology*; D006255:Rhinitis, Allergic, Seasonal / Q000473:pathology*
D007073:Immunoglobulin E
Die Darstellung IgE-assoziierter Zellen in der atopischen Nasenschleimhaut mit Hilfe der Immunperoxydase-Methode.