title: Effect of beta-hydroxybutyrate and acetoacetate on insulin and glucagon secretion from perfused rat pancreas.
abstract: To elucidate the physiological significance of ketone bodies on insulin and glucagon secretion, the direct effects of beta-hydroxybutyrate (BOHB) and acetoacetate (AcAc) infusion on insulin and glucagon release from perfused rat pancreas were investigated. The BOHB or AcAc was administered at concentrations of 10, 1, or 0.1 mM for 30 min at 4.0 ml/min. High-concentration infusions of BOHB and AcAc (10 mM) produced significant increases in insulin release in the presence of 4.4 mM glucose, but low-concentration infusions of BOHB and AcAc (1 and 0.1 mM) caused no significant changes in insulin secretion from perfused rat pancreas. BOHB (10, 1, and 0.1 mM) and AcAc (10 and 1 mM) infusion significantly inhibited glucagon secretion from perfused rat pancreas. These results suggest that physiological concentrations of ketone bodies have no direct effect on insulin release but have a direct inhibitory effect on glucagon secretion from perfused rat pancreas.
authors.forename: T
authors.initials: T
authors.lastname: Ikeda
authors.forename: T
authors.initials: T
authors.lastname: Yoshida
authors.forename: Y
authors.initials: Y
authors.lastname: Ito
authors.forename: I
authors.initials: I
authors.lastname: Murakami
authors.forename: O
authors.initials: O
authors.lastname: Mokuda
authors.forename: M
authors.initials: M
authors.lastname: Tominaga
authors.forename: H
authors.initials: H
authors.lastname: Mashiba
mesh_terms: D020155:3-Hydroxybutyric Acid; D000090:Acetoacetates / Q000494:pharmacology*; D000818:Animals; D005230:Fatty Acids, Nonesterified / Q000032:analysis; D005934:Glucagon / Q000378:metabolism*; D006885:Hydroxybutyrates / Q000494:pharmacology*; D066298:In Vitro Techniques; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D008066:Lipolysis; D008297:Male; D010179:Pancreas / Q000378:metabolism*; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D000090:Acetoacetates; D005230:Fatty Acids, Nonesterified; D006885:Hydroxybutyrates; D007328:Insulin; C016635:acetoacetic acid; D005934:Glucagon; D020155:3-Hydroxybutyric Acid