title: Mapping of the constitutive lysyl-tRNA synthetase gene of Escherichia coli K-12.
abstract: The constitutive lysyl-tRNA synthetase gene (lysS) was mapped at 62.1 min on the Escherichia coli chromosome by a combination of conjugation and transduction, with physical confirmation by two-dimensional gel electrophoresis. Revertant analysis suggests that the altered isoelectric point and the low amount of the mutant LysS protein may be due to a single mutational event.
authors.affiliation: Department of Biological Sciences, St. John's University, Jamaica, New York 11439.
authors.forename: R V
authors.initials: RV
authors.lastname: Emmerich
authors.forename: I N
authors.initials: IN
authors.lastname: Hirshfield
mesh_terms: D000604:Amino Acyl-tRNA Synthetases / Q000235:genetics*; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial / Q000502:physiology; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005796:Genes; D005798:Genes, Bacterial; D005838:Genotype; D008250:Lysine-tRNA Ligase / Q000235:genetics*; D010641:Phenotype
chemical_list: D000604:Amino Acyl-tRNA Synthetases; D008250:Lysine-tRNA Ligase