title: Preparation of positional renal slices for study of cell-specific toxicity.
abstract: To reduce structural complexity, rabbit kidneys were sliced perpendicular to their cortical-papillary axis to isolate four distinct cell groupings. This positional orientation allows identification of each renal cell type based on its location within the slice. A mechanical slicer was used to make several precision-cut slices rapidly from an oriented cylindrical core of renal tissue, with minimal tissue trauma. Slices were then submerged under a gently circulating oxygenated media in a fritted glass support system that maintains viability (intracellular K+/DNA ratio) and structural integrity (histology) for at least 30 h. A high dose of mercuric chloride (10(-3) M) was used to demonstrate the structural and biochemical changes of intoxicated slices. This method provides a controlled subchronic in vitro system for the study of the individual cell types involved in cell-specific renal toxicities and may also be a useful tool for addressing other pharmacological and physiological research questions.
authors.forename: C E
authors.initials: CE
authors.lastname: Ruegg
authors.forename: A J
authors.initials: AJ
authors.lastname: Gandolfi
authors.forename: R B
authors.initials: RB
authors.lastname: Nagle
authors.forename: C L
authors.initials: CL
authors.lastname: Krumdieck
authors.forename: K
authors.initials: K
authors.lastname: Brendel
mesh_terms: D000818:Animals; D006652:Histological Techniques; D066298:In Vitro Techniques; D007668:Kidney / Q000032:analysis / Q000166:cytology* / Q000187:drug effects; D008297:Male; D011188:Potassium / Q000032:analysis; D011817:Rabbits
chemical_list: D011188:Potassium