Preparation of immunoperoxidase-labelled wholemounts of invertebrate brains.
An immunoperoxidase method is described for the immunocytochemical labelling of whole cerebral ganglia of the crayfish to reveal serotonin-containing neurons. Careful desheathing, unusually long immersion in antibody reagents, the inclusion of blocking reagent in every incubation and extensive washing produce immunolabelled wholemount preparations with very low background. The entire neurotransmitter system is consistently labelled and single identifiable neurons can be repeatedly recognized.
Aitken
A R
AR
School of Anatomy, University of New South Wales, Kensington, Australia.
Sandeman
R E
RE
Sandeman
D C
DC
D000818:Animals; D003400:Astacoidea / Q000033:anatomy & histology*; D001360:Avidin; D001710:Biotin; D005724:Ganglia / Q000033:anatomy & histology*; D006652:Histological Techniques; D007124:Immunoenzyme Techniques
D001360:Avidin; D001710:Biotin