Standardization of procedures for ectopic marrow grafting. II. Influence on recipients of radiation dose and field size.
The ectopic implantation of mouse marrow to the kidney capsule offers considerable scope as an assay of the hemopoietic microenvironment. Our previous work has shown that whole-body irradiation of the graft recipient prior to implantation results in superior ossicle formation in the kidney of the host. Here we report that a range of irradiation doses over a 4-Gy threshold are equivalent with respect to conditioning the graft recipient. We also show that two distinct and separable influences affect graft growth in the irradiated recipient, namely, a local effect brought about in the irradiated kidney (and restricted to it) and secondly, a systemic effect resulting from irradiation of sites other than the kidney, which nevertheless affects ossicle growth in the shielded renal capsule.
Molineux
G
G
Schofield
R
R
Hendry
J H
JH
Testa
N G
NG
D000818:Animals; D016026:Bone Marrow Transplantation; D003114:Colony-Forming Units Assay / Q000379:methods; D004307:Dose-Response Relationship, Radiation; D005260:Female; D006412:Hematopoietic Stem Cells / Q000528:radiation effects; D007668:Kidney / Q000528:radiation effects*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D012737:Sex Factors; D014180:Transplantation / Q000379:methods; D014916:Whole-Body Irradiation