title: Tissue c-myc protein expression and immune response in systemic lupus erythematosus.
abstract: Frozen sections of kidney tissue from 12 patients with systemic lupus erythematosus were examined for the expression of oncogene proteins. Polyclonal rabbit antibody to c-myc peptide or to whole 65,000 D c-myc protein, was used to identify c-myc protein within tissue sections. Patterns of nuclear staining in the Hep-2 cell line were similar to those seen with monospecific human SLE sera showing anti-Sm reactivity. In addition c-myc staining was not abolished by prior incubation of tissue sections with human serum containing anti-Sm antibodies. Five of 12 kidney biopsy tissues from patients with systemic lupus erythematosus (SLE) showed positive speckled c-myc protein staining within nuclei of monocyte/macrophage cells of glomerular tufts. Positive staining was in all instances completely abolished by prior absorption of anti-c-myc antibody with c-myc protein. No c-myc protein was identified within SLE immune complex deposits.
authors.affiliation: Department of Medicine, University of New Mexico School of Medicine, Albuquerque 87131.
authors.forename: G
authors.initials: G
authors.lastname: Husby
authors.forename: R C
authors.initials: RC
authors.lastname: Williams
authors.forename: R A
authors.initials: RA
authors.lastname: Watt
authors.forename: S G
authors.initials: SG
authors.lastname: West
authors.forename: K S
authors.initials: KS
authors.lastname: Tung
mesh_terms: D000328:Adult; D000368:Aged; D000936:Antigen-Antibody Complex / Q000032:analysis; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007124:Immunoenzyme Techniques; D007668:Kidney / Q000032:analysis*; D008180:Lupus Erythematosus, Systemic / Q000276:immunology / Q000378:metabolism*; D008223:Lymphoma / Q000032:analysis; D008264:Macrophages / Q000032:analysis; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000032:analysis; D011518:Proto-Oncogene Proteins / Q000032:analysis*
chemical_list: D000936:Antigen-Antibody Complex; D011518:Proto-Oncogene Proteins