A direct assay for detection of chemically induced changes in the rejoining kinetics of radiation induced DNA strand breaks.
A simple and sensitive procedure for testing various chemicals affecting DNA repair is presented. Cells, either labelled with [3H]thymidine or [14C]thymidine, were drug-treated or used as references cells. Both cell populations were irradiated with 5 Gy. The number of DNA breaks were determined, after mixing of drug-treated and reference cells of different labelling, at various intervals by the DNA unwinding technique and the drug-dependent DNA breaks were calculated. The drugs benzamide, 3-aminobenzamide, novobiocin and 9-beta-D-arabinofuranosyladenine (araA), all known to affect DNA repair, were used to study their effect on the number of DNA strand breaks with the presented technique. It was found that the assay improved the accuracy in determining the influence of DNA repair inhibitors compared to indirect measurements.
Sandström
B E
BE
Division of Cell- and Microbiology, National Defence Research Institute, Umeå, Sweden.
Johanson
K J
KJ
D000818:Animals; D001549:Benzamides / Q000494:pharmacology*; D002250:Carbon Radioisotopes; D002460:Cell Line; D003561:Cytarabine / Q000494:pharmacology*; D004247:DNA / Q000187:drug effects / Q000528:radiation effects*; D004249:DNA Damage; D004260:DNA Repair / Q000187:drug effects*; D004261:DNA Replication / Q000187:drug effects / Q000528:radiation effects*; D007700:Kinetics; D009675:Novobiocin / Q000494:pharmacology*; D011865:Radioisotope Dilution Technique; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
D001549:Benzamides; D002250:Carbon Radioisotopes; D003561:Cytarabine; D014316:Tritium; D009675:Novobiocin; C037689:benzamide; C025160:3-aminobenzamide; D004247:DNA; D013936:Thymidine