[Affinity modification of Escherichia coli ribosomes by 4-[(N-2-chloroethyl)N-methylamino]benzyl-5'-phosphamide hexauridylate in a complex stabilized by codon-anticodon interactions on P and A sites].
Affinity labeling of E. coli ribosomes with 4-[(N-2-chloroethyl)-N-methylamino] benzyl-5'-phosphamide of hexauridylate was studied within the complex containing tRNAPhe at P site and Phe-tRNAPhe at A site directed by EF-Tu and GTP. Ribosomal proteins as well as rRNA both in 30S and 50S subunits were found to be labelled within the complex. Labeled proteins were identified as S3, S9 and L2. Selectivity of affinity labeling with mRNA analogs was shown to depend on the functional state of the ribosomes. Modification was more selective within the complex stabilized by codon-anticodon interaction both at A and P-sites than within the complex in which this interaction takes place preferentially at P site.
Gimautdinova
O I
OI
Karpova
G G
GG
D000926:Anticodon; D003062:Codon; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D009942:Organometallic Compounds / Q000378:metabolism / Q000494:pharmacology*; D012333:RNA, Messenger; D012335:RNA, Ribosomal / Q000378:metabolism; D012343:RNA, Transfer; D012360:RNA, Transfer, Phe / Q000378:metabolism; D012269:Ribosomal Proteins / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism*; D014500:Uracil Nucleotides / Q000494:pharmacology*; D014542:Uridine Monophosphate / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology*
D000926:Anticodon; D003062:Codon; D009942:Organometallic Compounds; D012333:RNA, Messenger; D012335:RNA, Ribosomal; D012360:RNA, Transfer, Phe; D012269:Ribosomal Proteins; D014500:Uracil Nucleotides; D012343:RNA, Transfer; D014542:Uridine Monophosphate
Affinnaia modifikatsiia ribosom Escherichia coli 4-[(N-2-khlorétil)N-metilamino]benzil-5'-fosfamidom geksauridilata v komplekse, stabiliziruemom kodon-antikodonovym vzaimodeĭstviem v P- i A-uchastke.