title: [Expression of human leukocyte interferon type A in Saccharomyces cerevisiae under the control of regulatory elements of the yeast gene URA3].
abstract: Expression of a chromosomal gene for human leucocyte interferon A was obtained due to interaction of 5'-nontranslating region of a cloned interferon gene with the regulatory sequences from UNA3 yeast gene. The sequence of 5'-nontranslating region from interferon gene essential for its expression in yeast is localized within 130 b p. from the initiating codon. Due to increasing of plasmid stability and copy number a 60-fold increase. in interferon yield was obtained in yeast transformants reaching the level of 6 X 10(7) u X 1(-1). The data are presented supposing the existence of functional polycistronic mRNA in yeast.
authors.forename: A B
authors.initials: AB
authors.lastname: Nikoshkov
authors.forename: M L
authors.initials: ML
authors.lastname: Zlochevskiĭ
authors.forename: V A
authors.initials: VA
authors.lastname: Naroditskaia
authors.forename: M R
authors.initials: MR
authors.lastname: Eremashvili
authors.forename: I I
authors.initials: II
authors.lastname: Tolstorukov
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005786:Gene Expression Regulation; D005800:Genes, Fungal; D007370:Interferon Type I / Q000235:genetics*; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins; D012441:Saccharomyces cerevisiae / Q000235:genetics*
chemical_list: D007370:Interferon Type I; D011994:Recombinant Proteins
vernacular_title: Ekspressiia gena leĭkotsitarnogo interferona A cheloveka v drozhzhakh Saccharomyces cerevisiae pod kontrolem reguliatornykh élementov drozhzhevogo gena URA3.