Immunocytochemical localization of relaxin in the golden hamster (Mesocricetus auratus) during the last half of gestation.
The objective of this study was to determine the tissue source of relaxin in pregnant hamsters by immunocytochemical techniques. Ovarian, uterine, and placental tissues were recovered from hamsters on Days 8, 10, 12, 14, and 15 of gestation and processed for light microscopy. Relaxin immunoreactivity was localized in tissue sections by the avidin-biotin-peroxidase technique using antiserum to porcine relaxin. On Day 8 of gestation, relaxin immunoreactivity was localized in primary giant trophoblast cells (GTC-1s) adjacent to the uterine decidua. On Day 10, relaxin immunoreactivity was localized in GTC-1s, secondary giant trophoblast cells (GTC-2s) adjacent to the ectoplacental cone, and endometrial granulocytes in the wall of sheathed arteries. On Day 12, relaxin immunoreactivity was observed primarily in GTC-2s interspersed among cells of the placental trophospongium but not in cells of the placental labyrinth. The intensity of staining and number of relaxin immunoreactive GTCs increased between Days 12 and 14 but was decreased by Day 15 PM. Relaxin was not localized in uterine glands or corpora lutea. These observations suggest that the placenta is the tissue source of relaxin in pregnant hamsters.
Renegar
R H
RH
Department of Anatomy, School of Medicine, East Carolina University, Greenville, North Carolina 27858.
Cobb
A D
AD
Leavitt
W W
WW
D000818:Animals; D006224:Cricetinae / Q000502:physiology*; D005260:Female; D007124:Immunoenzyme Techniques; D008647:Mesocricetus / Q000502:physiology*; D010053:Ovary / Q000032:analysis*; D010920:Placenta / Q000032:analysis* / Q000378:metabolism; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000502:physiology*; D012065:Relaxin / Q000032:analysis* / Q000378:metabolism; D014599:Uterus / Q000032:analysis*
D012065:Relaxin