The construction and operation of a simple inexpensive slam freezing device for electron microscopy.
A simple and inexpensive slam freezing device has been constructed that allows consistently good rapid freezing of uncryoprotected tissue. The device employs a dry liquid-nitrogen-cooled cooper surface and a slamming plunger designed to provide rapid and uniform tissue to metal contact.
Allison
D P
DP
Daw
C S
CS
Rorvik
M C
MC
D000818:Animals; D003300:Copper; D005615:Freezing; D006652:Histological Techniques / Q000295:instrumentation*; D051379:Mice; D008854:Microscopy, Electron / Q000295:instrumentation / Q000379:methods; D009206:Myocardium / Q000648:ultrastructure*
D003300:Copper