[Isolation of conjugated sera for the immunofluorescence demonstration of swine parvovirus].
High-titer, specific serum against porcine parvovirus was obtained via hyperimmunization of rabbits, with the use of a Bulgarian isolate that had been partially purified after a known, modified technique. A specific, high-titer conjugate was produced for the immunofluorescence diagnosis of porcine parvoviruses. The microscopic observation of the lamellae of cell cultures, treated with the conjugate, revealed the presence of a specific, typically granulated perinuclear (mostly unilaterally) and, in some cases nuclear fluorescence--at negative reaction in the control preparations. Immunofluorescent light was also established in cell cultures infected with the virus at highest dilution. It showed that immunofluorescence microscopy could be employed to demonstrate even the lowest amounts of the virus.
Peev
Ia
Ia
Motovski
A
A
Aleksandrov
M
M
Rezashka
A
A
Losev
B
B
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D007106:Immune Sera / Q000302:isolation & purification*; D007114:Immunization; D008854:Microscopy, Electron; D010321:Parvoviridae / Q000276:immunology*; D011817:Rabbits; D013552:Swine / Q000382:microbiology*; D014776:Virus Cultivation
D000914:Antibodies, Viral; D007106:Immune Sera
Poluchavane na koniugirani serumi za imunofluorestsentno dokazavane na parvovirusa po svinete.