Expression of protein IIIa of human adenovirus type 2 in Escherichia coli.
We have constructed a plasmid encoding the protein IIIa gene of human adenovirus type 2. The gene was expressed under the control of the hybrid tac (trp-lac) promoter; the protein was synthesized at levels up to 5% of newly synthesized protein after IPTG induction. The protein IIIa produced in Escherichia coli has an apparent Mr on sodium dodecyl sulfate-polyacrylamide gels of 67 kDa, and was revealed with anti-adenovirus serum in Western blotting. The protein IIIa produced in bacteria was phosphorylated in the presence of [gamma-32P]ATP.
Cuillel
M
M
European Molecular Biology Laboratory, Grenoble, France.
Milleville
M
M
D'Halluin
J C
JC
D000260:Adenoviruses, Human / Q000235:genetics*; D036022:Capsid Proteins; D004926:Escherichia coli / Q000235:genetics; D010766:Phosphorylation; D011401:Promoter Regions, Genetic; D011499:Protein Processing, Post-Translational; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics*; D011994:Recombinant Proteins / Q000235:genetics*; D014764:Viral Proteins / Q000096:biosynthesis / Q000235:genetics*
D036022:Capsid Proteins; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D014764:Viral Proteins; C052227:protein IIIa, Human adenovirus type 2