Trial of a cysteine proteinase inhibitor, EST, in experimental chloroquine myopathy in rats.
The administration of 50 mg/kg/day of chloroquine to rats for 8 weeks produced the chloroquine myopathy characterized by autophagic vacuole formation and increases in lysosomal enzymes, especially cathepsins B & L. Coadministration of 10 mg/kg/day of a potent cysteine proteinase inhibitor, EST, and chloroquine prevented the induction of the chloroquine myopathy. Rats already suffering from the chloroquine myopathy were treated with 10 mg/kg/day of EST together with chloroquine injections for 5 weeks and also recovered remarkably from the myopathy. Thus, EST may be beneficial for myopathies associated with autophagic vacuoles.
Sugita
H
H
Higuchi
I
I
Sano
M
M
Ishiura
S
S
D000818:Animals; D002738:Chloroquine / Q000037:antagonists & inhibitors; D003546:Cysteine Endopeptidases; D010450:Endopeptidases; D007930:Leucine / Q000031:analogs & derivatives* / Q000627:therapeutic use; D008247:Lysosomes / Q000201:enzymology; D008297:Male; D009132:Muscles / Q000201:enzymology / Q000473:pathology; D009135:Muscular Diseases / Q000139:chemically induced* / Q000188:drug therapy / Q000473:pathology; D009929:Organ Size; D011480:Protease Inhibitors; D051381:Rats; D011919:Rats, Inbred Strains
D011480:Protease Inhibitors; D002738:Chloroquine; D010450:Endopeptidases; D003546:Cysteine Endopeptidases; D007930:Leucine; C108192:aloxistatin