Specific and cooperative binding of E. coli single-stranded DNA binding protein to mRNA.
Fluorometric titration of E. coli single-stranded DNA binding protein with various RNAs showed that the protein specifically and cooperatively binds to its own mRNA. The binding inhibited in vitro expression of ssb and bla but not nusA. This inhibition takes place at a physiological concentration of SSB. The function of the protein in gene regulation is discussed.
Shimamoto
N
N
Ikushima
N
N
Utiyama
H
H
Tachibana
H
H
Horie
K
K
D001665:Binding Sites; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism*; D010957:Plasmids; D011485:Protein Binding; D012333:RNA, Messenger / Q000378:metabolism*
D004268:DNA-Binding Proteins; D012333:RNA, Messenger