High frequency generalized transduction by miniMu plasmid phage.
Deletion derivatives of phage Mu which replicate as multicopy plasmids, and also transpose and package like Mu, have been developed for the in vivo cloning of bacterial genes. We show here that these miniMu plasmid phage are also efficient at generalized transduction and that both in vivo cloning and generalized transduction of a given gene can be accomplished in a single experiment.
Wang
B M
BM
Liu
L
L
Groisman
E A
EA
Casadaban
M J
MJ
Berg
C M
CM
D003090:Coliphages / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D005838:Genotype; D010641:Phenotype; D010957:Plasmids; D014161:Transduction, Genetic