[Energy aspects of the growth of Escherichia coli synchronized by starvation].
The quantitative determination of adenyl nucleotides based on the separation of their dansyl derivatives by thin layer chromatography has made it possible to study the dynamics of changes in the pool of ATP, ADP and AMP in Escherichia coli K-12 during its synchronous growth after glucose starvation. The energy parameters (the adenylate pool, energy charge, teh ATP/ADP ratio, the rates of oxygen uptake and ATP generation, the economic coefficients of oxygen and ATP utilization) were compared with changes in the growth characteristics (the rate of growth and biomass concentration). This comparison allowed the authors to draw the conclusion about the uncoupled constructive and energy metabolism and about the possible regulatory role of energy parameters in the synchronised culture growth.
Tkachenko
A G
AG
Chudinov
A A
AA
D000227:Adenine Nucleotides / Q000378:metabolism; D001431:Bacteriological Techniques; D003470:Culture Media; D004734:Energy Metabolism; D004926:Escherichia coli / Q000254:growth & development* / Q000378:metabolism; D005947:Glucose / Q000172:deficiency; D007700:Kinetics
D000227:Adenine Nucleotides; D003470:Culture Media; D005947:Glucose
Energeticheskie aspekty razvitiia Escherichia coli, sinkhronizirovannoĭ golodaniem.