Ontogeny of ovine lymphocytes. II. An immunohistological study on the development of T lymphocytes in the sheep fetal spleen.
The development of T and B lymphocytes in the ovine fetal spleen was studied immunohistologically using a panel of monoclonal antibodies. A specific sequence of appearance of lymphocyte markers on cells was observed. At 43-44 days of gestation, SBU-T1- and SBU-T8-positive lymphocytes were present in low numbers. However, no SBU-T4, 20.96-, 25.69-, 38.38-, or 46.66-positive lymphocytes were seen until 50-55 days of gestation. Surface immunoglobulin (sIg) was first detected on fetal spleen cells at 45-50 days of gestation. SBU-T19 lymphocytes appeared later in gestation, being observed in fetal spleens at 57 days gestational age (g.a.). The distribution of T cells, B cells and MHC antigens in the developing spleen of the ovine fetus is described.
Maddox
J F
JF
Department of Veterinary Preclinical Sciences, University of Melbourne, Parkville, Victoria, Australia.
Mackay
C R
CR
Brandon
M R
MR
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology* / Q000276:immunology; D002454:Cell Differentiation; D005865:Gestational Age; D007124:Immunoenzyme Techniques; D012756:Sheep / Q000196:embryology* / Q000276:immunology; D013154:Spleen / Q000196:embryology* / Q000276:immunology; D013601:T-Lymphocytes / Q000166:cytology* / Q000276:immunology