Tear and serum IgE concentrations by Tandem-R IgE immunoradiometric assay in allergic patients.
The authors studied a population of 39 allergic and 15 nonallergic patients, and determined their tear and serum IgE concentrations. Samples of tear and serum were tested for IgE by the Tandem-R immunoradiometric assay, which uses monoclonal antibody to produce a specific assay for IgE. The serum IgE levels in the study group showed a range from 23,280 to 16 IU/ml compared with controls of 72 to 2 IU/ml. Tear IgE in the study group varied from 159 IU/ml to less than 1 IU/ml compared with controls of 8 IU/ml to less than 1 IU/ml. A statistically significant correlation between tear and serum IgE exists in the allergic patients with eye symptoms. It also exists when serum IgE was greater than 100 IU/ml, the tear IgE greater than 4 IU/ml, or when both the serum IgE was greater than 100 IU/ml and the tear IgE greater than 4 IU/ml.
Insler
M S
MS
LSU Eye Center, New Orleans 70112.
Lim
J M
JM
Queng
J T
JT
Wanissorn
C
C
McGovern
J P
JP
D000293:Adolescent; D000328:Adult; D000368:Aged; D005260:Female; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology*; D007073:Immunoglobulin E / Q000032:analysis*; D007158:Immunologic Techniques; D008297:Male; D008875:Middle Aged; D011874:Radiometry; D013666:Tears / Q000276:immunology*
D007073:Immunoglobulin E