title: Effects of food restriction on lactate production from glucose by rat adipocytes.
abstract: We investigated the effects of acute food deprivation on the rates of glucose metabolism and lactate production by epididymal fat cells isolated from 2- and 8-mo-old Wistar rats in one of three nutritional states: fed ad libitum, restricted to 50% of ad libitum, or fasted for 48 h. Food restriction did not affect total rates of basal glucose metabolism but significantly altered its pattern. In small fat cells from 2-mo-old rats, donor's food restriction led to decreased basal and insulin-stimulated glucose oxidation and fatty acid synthesis but led to increased lactate production, which increased from 4% of total glucose metabolized by cells from fed rats to 62% by cells from fasted rats. In large fat cells from 8-mo-old rats, donor's food restriction produced minimal changes in basal glucose metabolism and lactate production (already elevated at 37% of total) but caused insulin-stimulated lactate production to increase. These findings indicate that adipose tissue can produce significant amounts of lactate (with adipocyte enlargement and/or food deprivation) and may play an important role in extrahepatic generation of triose intermediates and in the Cori cycle.
authors.affiliation: Department of Medicine, Emory University School of Medicine, Atlanta, Georgia 30303.
authors.forename: S V
authors.initials: SV
authors.lastname: Thacker
authors.forename: M
authors.initials: M
authors.lastname: Nickel
authors.forename: M
authors.initials: M
authors.lastname: DiGirolamo
mesh_terms: D000273:Adipose Tissue / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D005508:Food Deprivation; D005947:Glucose / Q000378:metabolism*; D007328:Insulin / Q000494:pharmacology; D007773:Lactates / Q000096:biosynthesis*; D019344:Lactic Acid; D008297:Male; D009929:Organ Size; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D007328:Insulin; D007773:Lactates; D019344:Lactic Acid; D005947:Glucose