Tissue homogenization with sterile reinforced polyethylene bags for quantitative culture of Candida albicans.
A simple method of tissue homogenization with sterile reinforced polyethylene bags for quantitative fungal cultures was evaluated with mice infected with Candida albicans. This new method correlated well with standard methods (P less than or equal to 0.01) for quantifying viable fungus in homogenates of brain, kidney, spleen, liver, and lungs and may be applicable in clinical and experimental mycology laboratories.
Walsh
T J
TJ
McEntee
C
C
Dixon
D M
DM
D000818:Animals; D001921:Brain / Q000382:microbiology; D002176:Candida albicans / Q000254:growth & development*; D007668:Kidney / Q000382:microbiology; D008099:Liver / Q000382:microbiology; D008168:Lung / Q000382:microbiology; D051379:Mice; D009172:Mycology / Q000379:methods; D013154:Spleen / Q000382:microbiology