[Determination of cell surface antigens on cryostat sections with monoclonal antibodies].
Most monoclonal antibodies recognize antigens which do not survive conventional tissue processing: the use of frozen tissue sections and the immunofluorescence method overcome this obstacle but introduce other problems. Three improvements are reported here: the use of serum-free (substitute) "Ultroser Hy" as a culture medium for hybridomas, in order to diminish background staining and the diffusion artifact; the use of freon for freezing tissue sections, so as to slightly increase cellular morphology and staining; the use of a new immunofluorescent slide-mounting medium to enhance histologic preservation and immunohistologic contrast and to diminish fading of immunofluorescence.
Candelier
J J
JJ
Couillin
P
P
Roturier
M
M
Boué
A
A
D000911:Antibodies, Monoclonal / Q000276:immunology; D000954:Antigens, Surface / Q000032:analysis* / Q000276:immunology; D004622:Embryo, Mammalian; D005314:Embryonic and Fetal Development; D005455:Fluorescent Antibody Technique; D005615:Freezing; D006651:Histocytochemistry; D006801:Humans; D007668:Kidney / Q000166:cytology / Q000254:growth & development / Q000276:immunology; D009399:Nephrons / Q000166:cytology / Q000648:ultrastructure
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface
Mise en évidence des antigènes de surface cellulaire sur des coupes au cryostat grâce à des anticorps monoclonaux.