Preparation of positional renal slices for study of cell-specific toxicity.
To reduce structural complexity, rabbit kidneys were sliced perpendicular to their cortical-papillary axis to isolate four distinct cell groupings. This positional orientation allows identification of each renal cell type based on its location within the slice. A mechanical slicer was used to make several precision-cut slices rapidly from an oriented cylindrical core of renal tissue, with minimal tissue trauma. Slices were then submerged under a gently circulating oxygenated media in a fritted glass support system that maintains viability (intracellular K+/DNA ratio) and structural integrity (histology) for at least 30 h. A high dose of mercuric chloride (10(-3) M) was used to demonstrate the structural and biochemical changes of intoxicated slices. This method provides a controlled subchronic in vitro system for the study of the individual cell types involved in cell-specific renal toxicities and may also be a useful tool for addressing other pharmacological and physiological research questions.
Ruegg
C E
CE
Gandolfi
A J
AJ
Nagle
R B
RB
Krumdieck
C L
CL
Brendel
K
K
D000818:Animals; D006652:Histological Techniques; D066298:In Vitro Techniques; D007668:Kidney / Q000032:analysis / Q000166:cytology* / Q000187:drug effects; D008297:Male; D011188:Potassium / Q000032:analysis; D011817:Rabbits
D011188:Potassium