A ribosome-associated inhibitor of in vitro nonsense suppression in [psi-] strains of yeast.
All classes of tRNA-mediated nonsense suppression are much more efficient in yeast cell-free lysates prepared from a [psi+] strain than in those prepared from an isogenic [psi-] strain. Mixed [psi+]/[psi-] lysates do not support efficient suppression. Fractionation of the [psi-] lysate demonstrated the presence of an inhibitor of in vitro suppression that is loosely associated with the 80 S ribosome. The data indicate that the inhibitor is a factor involved in the termination of translation in this simple eukaryote.
Tuite
M F
MF
Biological Laboratory, University of Kent, Canterbury, England.
Cox
B S
BS
McLaughlin
C S
CS
D014176:Protein Biosynthesis; D012331:RNA, Fungal / Q000235:genetics; D012343:RNA, Transfer / Q000235:genetics; D012269:Ribosomal Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D012270:Ribosomes / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D013489:Suppression, Genetic / Q000187:drug effects*
D012331:RNA, Fungal; D012269:Ribosomal Proteins; D012343:RNA, Transfer