Microdissection and microcloning from the proximal region of mouse chromosome 7: isolation of clones genetically linked to the pudgy locus.
Microdissection and microcloning have been utilized in order to create a bank of clones from the proximal region of mouse chromosome 7. Several important loci map to this area, including the albino locus (c), pink-eye dilution (p), and the developmental mutant, pudgy (pu). By use of interspecific crosses between Mus musculus domesticus and Mus spretus, we have generated backcross progeny segregating for the mutations chinchilla (cch) and pink-eye dilution (p). Exploiting the evolutionary divergence between the two species, we have analyzed the inheritance of restriction fragment length variants of three microclones and their linkage to the two markers cch and p, respectively. All three clones studied map to the dissected region, and as such also show genetic linkage to the pudgy locus. This bank of chromosome 7-derived microclones should provide molecular start points for the isolation of a variety of developmental loci of unknown gene product, including the pudgy locus.
Greenfield
A J
AJ
Department of Biochemistry, St. Mary's Hospital Medical School, University of London, United Kingdom.
Brown
S D
SD
D000818:Animals; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004926:Escherichia coli; D005260:Female; D008040:Genetic Linkage; D005819:Genetic Markers; D008214:Lymphocytes / Q000166:cytology; D008297:Male; D008677:Metaphase; D051379:Mice
D005819:Genetic Markers