title: Preparation and characterization of bovine growth hormones produced in recombinant Escherichia coli.
abstract: Two analogues of bovine growth hormone (BGH) have been produced in Escherichia coli by recombinant DNA techniques. In analogue Delta-1, the N-terminal alanine residue of the full-length bovine sequence is replaced by methionine. In analogue Delta-9, which is expressed at much higher levels than is Delta-1, the full-length bovine sequence is truncated at the N-terminus by eight residues and there is a serine-for-glycine substitution in the first position of the truncated protein. Both analogues, which were characterized by isoelectric focusing (i.e.f.), polyacrylamide-gel electrophoresis in the presence of SDS (SDS/PAGE), amino acid analysis and N-terminal amino acid sequence determination using combined g.l.c.-m.s., are compared with BGH isolated from pituitaries. In contrast with pituitary-derived BGH, the recombinant-derived proteins are homogeneous on SDS/PAGE and on i.e.f. In a radioimmunoassay, a radioreceptor assay and a bioassay in vivo (rat tibia), Delta-9 BGH showed very similar characteristics to the pituitary-derived hormone. Similar results have also been obtained with the Delta-1 analogue.
authors.affiliation: Biogen S.A., Geneva, Switzerland.
authors.forename: P T
authors.initials: PT
authors.lastname: Wingfield
authors.forename: P
authors.initials: P
authors.lastname: Graber
authors.forename: G
authors.initials: G
authors.lastname: Buell
authors.forename: K
authors.initials: K
authors.lastname: Rose
authors.forename: M G
authors.initials: MG
authors.lastname: Simona
authors.forename: B D
authors.initials: BD
authors.lastname: Burleigh
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002417:Cattle; D004220:Disulfides / Q000032:analysis; D004926:Escherichia coli / Q000235:genetics; D013006:Growth Hormone / Q000235:genetics / Q000302:isolation & purification*; D007525:Isoelectric Focusing; D013058:Mass Spectrometry; D011863:Radioimmunoassay; D011995:Recombination, Genetic; D013438:Sulfhydryl Compounds / Q000032:analysis
chemical_list: D000596:Amino Acids; D004220:Disulfides; D013438:Sulfhydryl Compounds; D013006:Growth Hormone