Localization of a developmentally regulated neuron-specific protein S54 in dendrites as revealed by immunoelectron microscopy.
We sought to determine the ultrastructural localization of the developmentally regulated neuron-specific protein S54 in the chicken cerebellar cortex and optic tectum. The brains were fixed by perfusion with paraformaldehyde and glutaraldehyde. Frozen sections were immunocytochemically labeled with a monoclonal antibody to S54 protein. The immunoreactivity for S54 protein was localized in dendrites. No immunoreactivity for S54 protein was detected in axons and their presynaptic terminals.
Shirao
T
T
Inoue
H K
HK
Kano
Y
Y
Obata
K
K
D000818:Animals; D002525:Cerebellar Cortex / Q000032:analysis* / Q000648:ultrastructure; D002645:Chickens; D003712:Dendrites / Q000032:analysis*; D005260:Female; D006651:Histocytochemistry; D007158:Immunologic Techniques; D008854:Microscopy, Electron; D009479:Neuropeptides / Q000032:analysis*; D013477:Superior Colliculi / Q000032:analysis* / Q000648:ultrastructure
D009479:Neuropeptides; C053386:drebrins