Kinetics of recB-dependent repair: relationship to post-UV inactivation of the prophage.
By making use of the temperature-sensitive mutant recB270, we showed that the RecBCD enzyme is needed for repair between 1 and 4 h after UV exposure. recB-dependent prophage inactivation (Petranović et al. (1984), Mol. Gen. Genet., 196, 167-169) takes place in all dying cells during the same period of time. The kinetics of decrease in the yield of recombinants in phage-propage crosses resemble those of prophage inactivation in UV-irradiated bacteria. This indicates that recombination processes (including site-specific recombination required for prophage excision) are blocked in cells destined to die. On the basis of our results, we suggest that a large fraction of damaged cells is rescued by the RecA-RecBCD recombination pathway. If repair is unsuccessful, RecA-RecBCD recombination intermediates persist in the irradiated cells leading to prophage inactivation.
Trgovcević
Z
Z
Petranović
D
D
Salaj-Smic
E
E
Petranović
M
M
D003090:Coliphages / Q000201:enzymology / Q000235:genetics / Q000528:radiation effects*; D004260:DNA Repair; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics / Q000528:radiation effects*; D029968:Escherichia coli Proteins; D043211:Exodeoxyribonuclease V; D005090:Exodeoxyribonucleases / Q000378:metabolism*; D007700:Kinetics; D014466:Ultraviolet Rays
D029968:Escherichia coli Proteins; D005090:Exodeoxyribonucleases; D043211:Exodeoxyribonuclease V; C468322:exodeoxyribonuclease V, E coli