title: A significant increase of lysophosphatidylinositol 4-phosphate with insulin in isolated rat fat cells.
abstract: We studied the effects of insulin on the incorporation of 32Pi into phospholipids in rat fat cells. When the cells were treated with insulin, a new radioactive phospholipid was detected on thin layer chromatography. The substance migrated slower than phosphatidylinositol 4,5-bisphosphate and was hardly detectable in the absence of insulin. This effect of insulin was both time- and dose-dependent with half-maximal stimulation at 120 microU/ml. Pretreatment of insulin with anti-insulin antibody or the cells with anti-insulin receptor antibody inhibited the effect of insulin. The product of phosphatidylinositol 4-phosphate hydrolyzed by phospholipase A2 was coincided with the substance on thin layer chromatography. Quinacrine inhibited the formation of the substance in a dose-dependent manner. These results suggested that insulin stimulates the generation of lysophosphatidylinositol 4-phosphate through the insulin-receptor interaction.
authors.affiliation: Second Department of Internal Medicine, Hokkaido University, School of Medicine, Sapporo, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Kuroda
authors.forename: H
authors.initials: H
authors.lastname: Nakayama
authors.forename: T
authors.initials: T
authors.lastname: Ishibashi
authors.forename: S
authors.initials: S
authors.lastname: Aoki
authors.forename: S
authors.initials: S
authors.lastname: Tushima
authors.forename: S
authors.initials: S
authors.lastname: Nakagawa
mesh_terms: D000273:Adipose Tissue / Q000032:analysis / Q000378:metabolism*; D000818:Animals; D000906:Antibodies / Q000276:immunology; D002855:Chromatography, Thin Layer; D007328:Insulin / Q000276:immunology / Q000378:metabolism / Q000494:pharmacology*; D008246:Lysophospholipids / Q000096:biosynthesis*; D008297:Male; D010741:Phospholipases A / Q000378:metabolism; D054467:Phospholipases A2; D010743:Phospholipids / Q000096:biosynthesis; D011796:Quinacrine / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D011972:Receptor, Insulin / Q000187:drug effects / Q000276:immunology / Q000378:metabolism
chemical_list: D000906:Antibodies; D007328:Insulin; D008246:Lysophospholipids; D010743:Phospholipids; C052213:lysophosphatidylinositol 4-phosphate; D011972:Receptor, Insulin; D010741:Phospholipases A; D054467:Phospholipases A2; D011796:Quinacrine