A comparative study of immuno-blotting techniques for the detection of Gc-subtypes after isoelectric focusing on agarose and polyacrylamide gels.
A comparison of separation and detection techniques has been carried out to determine the most suitable combination for use in Gc-subtyping. The best results (i.e., high sensitivity, distinct bands, especially with reference to the 1S and 1F separation) were achieved using isoelectric focusing in polyacrylamide gel (pH 4.5-5.4) followed by transfer to nitrocellulose membrane by electroblotting and finally detection with enzyme-linked antibody complex.
Rand
S
S
Ritter
P
P
Kohfahl
A
A
Brinkmann
B
B
D004586:Electrophoresis; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007124:Immunoenzyme Techniques; D007158:Immunologic Techniques; D007525:Isoelectric Focusing; D014809:Vitamin D-Binding Protein / Q000235:genetics*
D014809:Vitamin D-Binding Protein