title: Localization of the T4 phage ribonucleotide reductase B1 subunit gene and the nucleotide sequence of its upstream and 5' coding regions.
abstract: The nucleotide (nt) sequence in a 757-bp [corrected] segment downstream from the intron-containing T4 phage thymidylate synthase gene (td) has been determined. This region was found to contain two open reading frames (ORFs). The first ORF(ORF2) [corrected] 261 bp [corrected] in length, is 24 [corrected] nt downstream from the td gene. The second ORF(ORF3) [corrected]) is 200 bp long at 558 [corrected] nt from the td gene and extends to the end of the Eco RI fragment. The amino acid (aa) sequence (66 aa residues) deduced from the second truncated ORF shows 59% homology to the sequence of the N-terminal portion of the ribonucleotide reductase large subunit of either Escherichia coli (B1 subunit) or mouse (M1 subunit). This tentatively identifies the truncated gene to be the 5' end of the T4 phage ribonucleotide reductase subunit B1 (nrdA) gene and pinpoints its exact location on the T4 phage genomic map. Southern hybridization analysis suggests good sequence homology among the nrdA genes of various T-even phages.
authors.affiliation: Wadsworth Center for Laboratories and Research, New York State Department of Health, Albany 12201.
authors.forename: F K
authors.initials: FK
authors.lastname: Chu
authors.forename: G F
authors.initials: GF
authors.lastname: Maley
authors.forename: A M
authors.initials: AM
authors.lastname: Wang
authors.forename: F
authors.initials: F
authors.lastname: Maley
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005091:Exons; D005796:Genes; D005814:Genes, Viral; D007438:Introns; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D010957:Plasmids; D012264:Ribonucleotide Reductases / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D013604:T-Phages / Q000201:enzymology / Q000235:genetics*
chemical_list: D046911:Macromolecular Substances; D012264:Ribonucleotide Reductases