An altered lectin binding to mucus glycoprotein in goblet cells of human tracheobronchial epithelium among former mustard-gas workers.
Lectins, which are well known to have an ability to bind with specific carbohydrate residues of glycoprotein, have been used to examine cellular changes associated with malignant transformation. For the analysis of mucus glycoprotein of goblet cells in the tracheobronchial epithelium, 192 paraffin-embedded sections from 54 autopsy cases including the cases with a history of mustard-gas (MG) exposure were stained with seven plant lectins using PAP method. PNA binding with no neuraminidase treatment as well as BSA-1 binding was observed most frequently in MG-exposed lung cancer cases. The proportion of cases positive for SBA binding in MG-exposed and/or lung cancer cases had a statistical difference from non-MG-exposed non-lung cancer cases. These observations may indicate a large heterogeneity in oligosaccharide chains of mucus glycoprotein and suggest its incomplete or abnormal synthesis, which is most likely to be due to previous exposure to carcinogen, such as MG.
Inai
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K
Kou
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E
Nambu
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S
Tokuoka
S
S
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001980:Bronchi / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D006023:Glycoproteins / Q000378:metabolism*; D006801:Humans; D007124:Immunoenzyme Techniques; D037102:Lectins / Q000378:metabolism; D008175:Lung Neoplasms / Q000139:chemically induced / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009093:Mucus / Q000378:metabolism; D009150:Mustard Compounds / Q000009:adverse effects*; D009151:Mustard Gas / Q000009:adverse effects*; D014132:Trachea / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*
D006023:Glycoproteins; D037102:Lectins; D009150:Mustard Compounds; C047874:gastric mucus glycoproteins; D009151:Mustard Gas