Separation of protein hormones.
The purpose of this review is to highlight modern techniques in HPLC and electrophoresis used for protein hormone separations. The advent of biotechnological methods for production of synthetic polypeptides and recombinant proteins will have a significant future impact on the types of therapeutics and metabolites that need to be monitored in the clinical laboratory. The protein hormone examples given in this work were selected because of the comprehensive body of separation science literature and not necessarily for their future importance in medicine. The intention was to present an array of general methods and techniques which may be useful to the clinical investigator for analysis of any protein hormone.
Pearson
J D
JD
McCroskey
M C
MC
DeWald
D B
DB
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004586:Electrophoresis; D006728:Hormones / Q000302:isolation & purification*; D006801:Humans; D011506:Proteins / Q000302:isolation & purification*
D006728:Hormones; D011506:Proteins