Random cloning of bent DNA segments from Escherichia coli chromosome and primary characterization of their structures.
A simple method for the selective detection of DNA segments containing a sequence-directed static bend was developed. Two-dimensional polyacrylamide gel electrophoresis performed at two different temperatures (60 degrees C and 10 degrees C) can effectively separate a bent DNA from a mixture of normal DNA. Using this method, a bank of plasmids carrying bent DNA inserts from the E. coli total chromosome was constructed. The primary characterization of a set of bent DNA segments randomly cloned from E. coli was presented.
Mizuno
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Laboratory of Microbiology, School of Agriculture, Nagoya University, Japan.
D001483:Base Sequence; D002876:Chromosomes, Bacterial / Q000502:physiology*; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics* / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010957:Plasmids
D004269:DNA, Bacterial