Characterization of a spontaneously transformed pulmonary embryonic rat (PER) epithelial cell line.
A spontaneously transformed pulmonary embryonic rat epithelial cell line (PER) is described in terms of growth, tumorigenicity, growth factor responsiveness and biosynthetic capacity. At low-passage subcultures, PER cells grew as a monolayer and did not form colonies in soft agar. After long-term subcultivation, they lost contact inhibition, became anchorage-independent and formed tumours in nude mice. Low concentrations of foetal calf serum permit the maximum growth rate. The multiplication and metabolic activity, assessed by 2-deoxy-D-glucose uptake, was significantly stimulated by growth factors. PER cells synthesized collagen types I, III, IV and V, laminin and fibronectin, and organized a pericellular matrix made up of only basement membrane components (type IV collagen and laminin) and fibronectin. These data enabled us to define PER cells as a transformed epithelial cell line evolving towards malignancy with long-term subcultivation. These cells appeared to be a valuable tool in studies of tumour cell-matrix interactions and regulation of growth factor receptors in tumorigenesis.
Paye
M
M
Laboratoire de Dermatologie Expérimentale, Université de Liège, Belgium.
Etievant
C
C
Michiels
F
F
Pierard
D
D
Nusgens
B
B
Lapière
C M
CM
D000818:Animals; D002455:Cell Division; D002460:Cell Line / Q000502:physiology*; D002471:Cell Transformation, Neoplastic / Q000648:ultrastructure*; D003094:Collagen / Q000096:biosynthesis; D004195:Disease Models, Animal; D004848:Epithelium / Q000648:ultrastructure; D005353:Fibronectins / Q000096:biosynthesis; D008168:Lung / Q000196:embryology / Q000648:ultrastructure*; D008856:Microscopy, Fluorescence; D008858:Microscopy, Phase-Contrast; D051381:Rats; D011919:Rats, Inbred Strains
D005353:Fibronectins; D003094:Collagen