Regulatory mutants of the aroF-tyrA operon of Escherichia coli K-12.
The regulatory region of the aroF-tyrA operon was fused to the chloramphenicol acetyltransferase (cat) gene on a plasmid vector. Expression of the cat gene was subject to repression by tyrR+. This fusion was used to isolate regulatory mutants with increased expression of the cat gene in which repression by tyrR+ was affected. Nucleotide sequencing of these mutants has led to the identification of three sites involved in the repression of aroF by tyrR+. The existence of a functional promoter divergently transcribing from the aroF regulatory region was also demonstrated by using the cat fusion vector. The expression of this promoter is also regulated by tyrR+.
Cobbett
C S
CS
Delbridge
M L
ML
D000123:Acetyltransferases / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics; D015500:Chloramphenicol O-Acetyltransferase; D004926:Escherichia coli / Q000235:genetics*; D005786:Gene Expression Regulation; D005809:Genes, Regulator; D009154:Mutation; D009875:Operator Regions, Genetic; D009876:Operon; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000235:genetics; D012097:Repressor Proteins / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic; D014443:Tyrosine / Q000235:genetics*
D001426:Bacterial Proteins; D011993:Recombinant Fusion Proteins; D012097:Repressor Proteins; D014157:Transcription Factors; D014443:Tyrosine; D000123:Acetyltransferases; D015500:Chloramphenicol O-Acetyltransferase