title: Human calcitonin gene-related peptide activates adenylate cyclase and releases prostacyclin from human umbilical vein endothelial cells.
abstract: 1 Endothelial cells of human umbilical vein were isolated and cultured in vitro. 2 In these cells there was a concentration-dependent release of prostacyclin and activation of adenylate cyclase by human calcitonin gene-related peptide (hCGRP). The concentration of hCGRP for half-maximum activation of adenylate cyclase (Kact) by hCGRP was 190 nM. 3 Bradykinin induced a ten fold greater release of prostacyclin than CGRP, but did not activate adenylate cyclase. 4 hCGRP may exert its potent vasodilator properties by stimulating release of vasorelaxant substances, including prostacyclin from endothelial cells.
authors.affiliation: Department of Clinical Pharmacology, Royal Postgraduate Medical School, London.
authors.forename: D
authors.initials: D
authors.lastname: Crossman
authors.forename: J
authors.initials: J
authors.lastname: McEwan
authors.forename: J
authors.initials: J
authors.lastname: MacDermot
authors.forename: I
authors.initials: I
authors.lastname: MacIntyre
authors.forename: C T
authors.initials: CT
authors.lastname: Dollery
mesh_terms: D015121:6-Ketoprostaglandin F1 alpha / Q000378:metabolism; D000262:Adenylyl Cyclases / Q000378:metabolism*; D015740:Calcitonin Gene-Related Peptide; D004727:Endothelium / Q000166:cytology / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects; D011464:Epoprostenol / Q000378:metabolism*; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D009131:Muscle, Smooth, Vascular / Q000378:metabolism*; D009479:Neuropeptides / Q000494:pharmacology*; D014471:Umbilical Veins / Q000378:metabolism; D014665:Vasodilator Agents / Q000494:pharmacology*
chemical_list: D009479:Neuropeptides; D014665:Vasodilator Agents; D015121:6-Ketoprostaglandin F1 alpha; D011464:Epoprostenol; D000262:Adenylyl Cyclases; D015740:Calcitonin Gene-Related Peptide