Synthesis of conjugates between luteinizing hormone releasing hormone (LH-RH) and N-acetyl-muramyl-L-alanyl-D-isoglutamine (MDP) models of totally synthetic vaccines.
Two glycopeptides associating the aminoacid sequence of LH-RH with MDP were prepared, using a Lys residue as a linker. These conjugates, N alpha MDP N epsilon (LH-RH)-Lys and N alpha MDP N epsilon (LH-RH)-Lys-NH2 obtained by condensation of fragments were synthesized by liquid as well as solid phase methods. Both compounds were able to induce anti LH-RH antibodies and immunological castration. They retained the immuno-adjuvant activity of MDP. Such antigen-adjuvant constructs, devoid of carrier and obtained by chemically defined and reproducible synthetic methods could offer suitable tools for structure-activity relationship studies aiming at defining synthetic vaccines.
Bernard
J M
JM
Gras-Masse
H
H
Drobecq
H
H
Tartar
A
A
Lefrancier
P
P
Hosmalin
A
A
Carelli
C
C
Chedid
L
L
D000119:Acetylmuramyl-Alanyl-Isoglutamine; D000818:Animals; D000917:Antibody Formation; D000941:Antigens; D004797:Enzyme-Linked Immunosorbent Assay; D006020:Glycopeptides; D007987:Gonadotropin-Releasing Hormone; D007202:Indicators and Reagents; D008297:Male; D051379:Mice; D013737:Testis / Q000187:drug effects / Q000378:metabolism; D013739:Testosterone / Q000378:metabolism; D014614:Vaccines, Synthetic
D000941:Antigens; D006020:Glycopeptides; D007202:Indicators and Reagents; D014614:Vaccines, Synthetic; D007987:Gonadotropin-Releasing Hormone; D013739:Testosterone; D000119:Acetylmuramyl-Alanyl-Isoglutamine