Mutations in the recD gene of Escherichia coli that raise the copy number of certain plasmids.
Chromosomal mutants were isolated in which, for several small plasmids, there was an increased amount of either covalently closed circular plasmid DNA or total plasmid DNA or both. The mutations were mapped to recD, which has been shown to affect exonuclease V activity and a variety of plasmid maintenance and replication functions. Our results suggest that rolling-circle plasmid replication can occur in recD mutants and that site-specific recombination can resolve the resulting linear multimers into covalently closed circular plasmid forms.
Seelke
R
R
Department of Biological Sciences, University of Wisconsin-Milwaukee 53201.
Kline
B
B
Aleff
R
R
Porter
R D
RD
Shields
M S
MS
D001345:Autoradiography; D004261:DNA Replication; D004269:DNA, Bacterial / Q000032:analysis*; D003720:Densitometry; D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D009154:Mutation; D010957:Plasmids
D004269:DNA, Bacterial