title: Quantification of 5- and 15-HPETE's by direct chemical ionization mass spectrometry.
abstract: This report describes the application of direct chemical ionization mass spectrometry (DCIMS) to the identification and quantification of 5- and 15-HPETEs. A unique feature of the method is use of a polyimide-coated fused silica fiber that allows vaporization of the hydroperoxides, with very low excess energy, into the plume of the chemical ionization reagent gas plasma. Mass spectra are obtained that allow identification of the nonreduced and nonderivatized free acid forms of 5- and 15-HPETE as well as their quantification from 1 microgram to 100 picograms.
authors.affiliation: Department of Anesthesia, Stanford University School of Medicine, CA 94305.
authors.forename: J
authors.initials: J
authors.lastname: Gut
authors.forename: G C
authors.initials: GC
authors.lastname: Jamieson
authors.forename: J R
authors.initials: JR
authors.lastname: Trudell
mesh_terms: D000818:Animals; D001095:Arachidonic Acids / Q000032:analysis*; D006801:Humans; D006893:Hydroxyeicosatetraenoic Acids / Q000032:analysis*; D015289:Leukotrienes; D008054:Lipid Peroxides / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D013055:Spectrophotometry, Infrared
chemical_list: D001095:Arachidonic Acids; D006893:Hydroxyeicosatetraenoic Acids; D015289:Leukotrienes; D008054:Lipid Peroxides; C022022:5-hydroxy-6,8,11,14-eicosatetraenoic acid; C025086:15-hydroperoxy-5,8,11,13-eicosatetraenoic acid