Detection of antibodies to Eperythrozoon ovis by the use of an enzyme-linked immunosorbent assay.
Specific antibody to Eperythrozoon ovis was detected by an enzyme-linked immunosorbent assay (ELISA) in the sera of infected sheep. In the presence of parasite antigen, positive control serum showed a reaction approximately eight times that of negative serum. When compared to an immunofluorescent antibody test (IFAT), the ELISA was eight times more sensitive. Positive control sera gave a titre of 1:3200 by IFAT and 1:25,600 by ELISA. Through the use of a reference titration curve ELISA could be used as a semi-quantitative system to determine antibody levels in test sera.
Lang
F M
FM
Victorian Department of Agriculture and Rural Affairs, Regional Veterinary Laboratory, Benalla, Australia.
Ferrier
G R
GR
Nicholls
T J
TJ
D000711:Anaplasmataceae Infections / Q000662:veterinary*; D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D009174:Mycoplasma / Q000276:immunology*; D009175:Mycoplasma Infections / Q000276:immunology / Q000662:veterinary*; D012756:Sheep; D012757:Sheep Diseases / Q000276:immunology* / Q000382:microbiology
D000907:Antibodies, Bacterial