Development and characterization of five monoclonal antibodies against neuronal cell surface antigens--evaluation of their use in cell separation by affinity chromatography.
Five monoclonal antibodies directed against rat neuronal cell surface proteins have been isolated and characterized following fusion of mouse SP2/O-Ag-14 myeloma cells and spleen cells from BALB/c mice immunized with neuron-specific membrane proteins. The antibodies displayed cell specificity in binding and elicited precipitation of different polypeptides from total neuronal cell surface proteins. These antibodies are of potential use in the isolation of a neuronal subpopulation by immunoaffinity chromatography. The work represents the application of hybridoma technology for the isolation of specific types of neuronal populations.
Chatterjee
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D
Mandal
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Sarkar
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D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification; D000954:Antigens, Surface / Q000032:analysis*; D001253:Astrocytes / Q000032:analysis; D001923:Brain Chemistry; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D002846:Chromatography, Affinity / Q000379:methods; D007158:Immunologic Techniques; D009419:Nerve Tissue Proteins / Q000032:analysis*; D009474:Neurons / Q000032:analysis* / Q000145:classification; D009836:Oligodendroglia / Q000032:analysis; D051381:Rats
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D009419:Nerve Tissue Proteins