Enzyme immunoassay measurement of thymosin beta 4.
An enzyme immunoassay for the measurement of thymosin beta 4, a chemically characterized thymic polypeptide, is described. A limited amount of reagent antiserum to synthetic thymosin beta 4 is incubated with liquid-phase thymosin beta 4. Unbound antibody is then incubated with solid-phase thymosin beta 4. The method is specific, sensitive, highly reproducible and capable of detecting as little as 2.5 ng/ml of thymosin beta 4.
Weller
F E
FE
Mutchnick
M G
MG
D000818:Animals; D000937:Antigen-Antibody Reactions; D002417:Cattle; D004306:Dose-Response Relationship, Immunologic; D007124:Immunoenzyme Techniques; D007700:Kinetics; D011485:Protein Binding; D013947:Thymosin / Q000031:analogs & derivatives* / Q000032:analysis / Q000276:immunology; D000099201:Thymosin beta(4)
D013947:Thymosin; D000099201:Thymosin beta(4)