Mutagenicity testing of human milk from smokers and non-smokers in the Salmonella/microsome test.
Human milk was tested in the Ames plate incorporation test using strain TA98. Pools from smokers and non-smokers respectively, as well as individual samples from 14 smokers and 15 controls were tested. No difference was found between milk from smokers and non-smokers in the concentrations and volumes used. Preliminary to this a cigarette smoke condensate (CSC) was tested. The CSC was then incubated with human milk which was fractionated, concentrated and the different fractions tested for mutagenic activity. 98% of the mutagenic effect added (measured as number of revertants caused by an equivalent volume of CSC) was recovered 52% of the revertant colonies were recovered from the fat fractions, 40% from the skimmed milk and 8% from a precipitate. Breast milk was also incubated with C14-benzo(a)pyrene and then fractionated by density gradient ultracentrifugation. 97% of the radioactivity was found in the lipid fraction.
Rivrud
G N
GN
Institute of Medical Genetics, University of Oslo, Norway.
Hagen
I
I
Børresen
A L
AL
D000818:Animals; D005260:Female; D006801:Humans; D008892:Milk; D008895:Milk, Human; D009152:Mutagenicity Tests; D009153:Mutagens / Q000494:pharmacology*; D009154:Mutation; D012016:Reference Values; D012486:Salmonella typhimurium / Q000187:drug effects; D012907:Smoking
D009153:Mutagens