title: Ligand-binding and idiotypic cross-reactions between anti-DNA antibodies and antibodies to Klebsiella K30 polysaccharide in patients with systemic lupus erythematosus or rheumatoid arthritis.
abstract: Antibodies to DNA and K30p were purified by affinity procedures and tested for their ability to cross-react with K30p and DNA, respectively. Anti-ssDNA antibodies were shown to react with solid-phase K30p and be inhibited in a dose-dependent manner by soluble ssDNA and K30p. Conversely, anti-K30p antibodies were found to bind immobilized ssDNA and be inhibited with soluble inhibitor. These results show that certain subpopulations of anti-ssDNA and anti-K30p antibodies overlap in their ligand-binding specificities. Idiotypic (Id) analysis showed that anti-K30p antibodies did not react significantly with an anti-Id reagent directed against a common anti-DNA framework Id (AM Id), thus clearly separating K30p-binding anti-DNA antibodies into an AM Id-negative population. When anti-DNA antibodies were probed with an anti-Id reagent directed against a framework Id present on anti-K30p antibodies (SP Id), substantial reactivity was found. Thus the SP Id identifies a subpopulation of antibodies capable of binding both K30p and DNA. These results show that a subset of anti-ssDNA antibodies cross-react with K30p antigen and are idiotypically related to a subset of antibodies reactive with K30p.
authors.forename: G D
authors.initials: GD
authors.lastname: Harkiss
authors.forename: F
authors.initials: F
authors.lastname: Hendrie
authors.forename: G
authors.initials: G
authors.lastname: Nuki
mesh_terms: D000888:Antibodies, Anti-Idiotypic / Q000276:immunology; D000974:Antibodies, Antinuclear / Q000378:metabolism*; D000907:Antibodies, Bacterial / Q000276:immunology; D000937:Antigen-Antibody Reactions; D000942:Antigens, Bacterial / Q000276:immunology*; D001172:Arthritis, Rheumatoid / Q000276:immunology*; D003429:Cross Reactions; D004277:DNA, Single-Stranded / Q000276:immunology; D006801:Humans; D007130:Immunoglobulin Idiotypes / Q000276:immunology; D007711:Klebsiella pneumoniae / Q000276:immunology*; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D011135:Polysaccharides, Bacterial / Q000276:immunology*
chemical_list: D000888:Antibodies, Anti-Idiotypic; D000974:Antibodies, Antinuclear; D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D004277:DNA, Single-Stranded; D007130:Immunoglobulin Idiotypes; D011135:Polysaccharides, Bacterial