Hyperthermia for treatment of human split-thickness skin. Differential effects on HLA-DR-expressing cells and keratinocytes.
Human split-thickness skin was hyperthermia-treated and then the tissue was trypsinized to obtain dispersed cell suspensions. Cells consisting mainly of keratinocytes, obtained from the treated epidermis, retained their capacity to grow in vitro. By contrast, HLA-DR-expressing cells, dispersed from the epidermis as well as the dermis, lost their capacity to stimulate allogeneic peripheral blood mononuclear leukocytes after heat treatment (1.5 hr at 45 degrees C). The heat-treated epidermal cells did not release a factor that inhibited allogeneic lymphocyte proliferation. Even though the cells expressing HLA-DR antigen were no longer capable of stimulating allogeneic lymphocytes after hyperthermia, they still expressed HLA-DR antigen on their surface up to 72 hr after exposure to heat. The inhibition of stimulation of allogeneic lymphocytes by heat treatment was at least partially reversed by the addition of exogenous interleukin-2, but not interleukin-1. Thus, functions of the various cell types present in split-thickness skin were differentially sensitive to heat, a characteristic of skin cells that may be useful in skin transplantation.
Morhenn
V B
VB
D002455:Cell Division; D002478:Cells, Cultured; D000078404:Epidermal Cells; D004817:Epidermis / Q000276:immunology; D006681:HLA-D Antigens / Q000276:immunology*; D006684:HLA-DR Antigens / Q000276:immunology*; D006358:Hot Temperature; D006801:Humans; D007111:Immunity, Cellular; D066298:In Vitro Techniques; D007375:Interleukin-1 / Q000494:pharmacology; D007376:Interleukin-2 / Q000494:pharmacology; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology*; D012867:Skin / Q000166:cytology / Q000276:immunology; D016038:Skin Transplantation
D006681:HLA-D Antigens; D006684:HLA-DR Antigens; D007375:Interleukin-1; D007376:Interleukin-2