[Effectiveness of substituting E. coli DNA-polymerase for DNA-polymerase A in oligonucleotide-directed mutagenesis].
The possibility to use the E. coli intact DNA polymerase I in the oligonucleotide-directed site-specific mutagenesis of DNA has been studied. Optimal conditions of the extension activity of this enzyme were found. We have shown that the substitution of the Klenow fragment of the E. coli DNA polymerase by the intact DNA polymerase I did not decrease the efficiency and fidelity of the oligonucleotide-directed mutagenesis.
Petrenko
V A
VA
Kipriianov
S M
SM
Semenova
L N
LN
Boldyrev
A N
AN
D004256:DNA Polymerase I / Q000235:genetics*; D004269:DNA, Bacterial / Q000096:biosynthesis* / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D007700:Kinetics; D009154:Mutation; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000235:genetics*; D011487:Protein Conformation
D004269:DNA, Bacterial; D009841:Oligonucleotides; D004256:DNA Polymerase I
Effektivnost' zameny DNK-polimerazy A DNK-polimerazoĭ I E. coli v oligonukleotidnapravlennom mutageneze.