A motility lesion in ColV+ Escherichia coli strains and its possible clinical significance.
The introduction of the ColV-K30 or ColV,I-K94 plasmid into Escherichia coli strains produced derivatives which had a motility lesion if grown without shaking at 37 degrees C. Although most ColV+ organisms from shaken cultures were motile, 80-90% of free unclumped organisms from static cultures were flagellate but non-motile. This plasmid effect was temperature-dependent with only those ColV+ organisms grown at 37 degrees C being affected; ColV+ organisms grown at 30 degrees C or below were predominantly motile. The motility lesion depended on the presence, in the ColV+ organisms, of transfer and colicin components together but not of the VmpA protein. Aside from the changed motility, there was extensive autoagglutination (clumping) of ColV+ organisms in static cultures, and the two phenomena (clumping and motility lesion) appeared to be governed by the same factors. The Flac plasmid of FI incompatibility group had a slight inhibitory effect on motility of strain 1829 and caused slight clumping, but representative plasmids of groups FII, FIII, FIV, C, H, I, K, M, N, P, W and X had no appreciable effect on either parameter. Non-motile ColV+ organisms regained motility on incubation with buffered detergent solutions, suggesting that an envelope change might be responsible for the altered motility. It can be hypothesized that ColV+ organisms in the intestine would be motile and hence able to reach the intestinal epithelium for invasion but that, once such organisms had reached the tissues and bloodstream, they would be predominantly non-motile and hence might be less susceptible to phagocytosis.
Tewari
R
R
Department of Botany and Microbiology, University College London.
Smith
D G
DG
Rowbury
R J
RJ
D001769:Blood / Q000382:microbiology; D002465:Cell Movement; D003087:Colicins / Q000096:biosynthesis; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D005407:Flagella / Q000648:ultrastructure; D006801:Humans; D007422:Intestines / Q000382:microbiology; D008855:Microscopy, Electron, Scanning; D010957:Plasmids; D013696:Temperature; D014774:Virulence
D003087:Colicins