Topological properties of DNA domains and interaction with RNA polymerase II.
We have analyzed the relationship between the alterations of the DNA structure induced by topological constraint and the template properties of promoters in vitro. A cause-effect relationship has been defined in several instances. Experimental protocols have been developed for the study of the topological properties of RNA polymerase II promoters. The goal of these studies is the definition of the intrinsic structural informations of DNA.
Negri
R
R
Dipartimento Genetica e Biologia Molecolare Facoltà Scienze Università La Sapienza, Roma, Italy.
Della Seta
F
F
Ficca
A G
AG
Camilloni
G
G
Di Mauro
E
E
D004247:DNA / Q000378:metabolism*; D004278:DNA, Superhelical; D009690:Nucleic Acid Conformation; D011401:Promoter Regions, Genetic; D012319:RNA Polymerase II / Q000378:metabolism*; D012045:Regulatory Sequences, Nucleic Acid; D012441:Saccharomyces cerevisiae / Q000235:genetics; D014158:Transcription, Genetic
D004278:DNA, Superhelical; D004247:DNA; D012319:RNA Polymerase II