Quantitative measurement of 5-, 12-, and 15-hydroxyeicosatetraenoic acid together with 12-hydroxyheptadecatrienoic acid by stable isotope dilution gas chromatography-negative ion chemical ionization-mass spectrometry.
A stable isotope dilution gas chromatography-negative ion chemical ionization-mass spectrometry assay for simultaneous quantitative measurement of 5-hydroxyeicosatetraenoic acid (HETE), 12-HETE, 15-HETE, and 12-hydroxyheptadecatrienoic acid in one single GC/MS run was established. 18O2-Labeled analogs as internal standards, prepared according to conventional procedures, were found to be useful for this application. A sample processing and derivatization sequence providing highly purified compounds with a recovery of 42.7% was elaborated. The detection limit was in the femtomole range.
Leis
H J
HJ
Malle
E
E
Mayer
B
B
Kostner
G M
GM
Esterbauer
H
H
Gleispach
H
H
D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D005231:Fatty Acids, Unsaturated / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006893:Hydroxyeicosatetraenoic Acids / Q000032:analysis*; D007201:Indicator Dilution Techniques; D007553:Isotope Labeling; D010103:Oxygen Isotopes
D005231:Fatty Acids, Unsaturated; D006893:Hydroxyeicosatetraenoic Acids; D010103:Oxygen Isotopes; C022022:5-hydroxy-6,8,11,14-eicosatetraenoic acid; C008033:12-hydroxy-5,8,10-heptadecatrienoic acid; D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; C025984:15-hydroxy-5,8,11,13-eicosatetraenoic acid