A whole mount method for sequential analysis of photoreceptor and ganglion cell topography in a single retina.
Photoreceptors (PR) in human and monkey retina are visible in whole mounts cleared with glycerol or dimethyl sulfoxide and viewed with Nomarski differential interference contrast microscopy. These preparations substantially decrease the large tissue volume changes associated with dehydration and sectioning and reveal many details of PR organization and cytology with great clarity. Tissue may be subsequently stained to reveal ganglion cells so that the topography of both cell types may be studied in the same retina.
Curcio
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Packer
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Kalina
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D000818:Animals; D003584:Cytological Techniques; D004121:Dimethyl Sulfoxide; D005990:Glycerol; D006801:Humans; D008251:Macaca; D010786:Photoreceptor Cells / Q000166:cytology*; D012160:Retina / Q000166:cytology*; D012165:Retinal Ganglion Cells / Q000166:cytology*
D005990:Glycerol; D004121:Dimethyl Sulfoxide