title: Coordinate initiation of chromosome and minichromosome replication in Escherichia coli.
abstract: Escherichia coli minichromosomes harboring as little as 327 base pairs of DNA from the chromosomal origin of replication (oriC) were found to replicate in a discrete burst during the division cycle of cells growing with generation times between 25 and 60 min at 37 degrees C. The mean cell age at minichromosome replication coincided with the mean age at initiation of chromosome replication at all growth rates, and furthermore, the age distributions of the two events were indistinguishable. It is concluded that initiation of replication from oriC is controlled in the same manner on minichromosomes and chromosomes over the entire range of growth rates and that the timing mechanism acts within the minimal oriC nucleotide sequence required for replication.
authors.forename: C E
authors.initials: CE
authors.lastname: Helmstetter
authors.forename: A C
authors.initials: AC
authors.lastname: Leonard
mesh_terms: D002453:Cell Cycle; D002455:Cell Division; D002876:Chromosomes, Bacterial / Q000378:metabolism*; D004261:DNA Replication; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D005798:Genes, Bacterial