Enzyme-linked immunofiltration assay for rapid serodiagnosis of syphilis.
A new rapid technique for detection of serum treponemal antibodies is described which is based on an enzyme-linked immunoassay using nitrocellulose as solid phase. With this technique antigen-antibody binding is accelerated by the filtration of the antibody solution through the antigen-coated nitrocellulose filter instead of its remaining over the solid phase for incubation. Test results are available in less than 15 min. Serum specimens from 255 syphilitics and 829 non-infected subjects were investigated. The sensitivity and specificity of the Treponema pallidum enzyme-linked immunofiltration assay were comparable to those of the Treponema pallidum haemagglutination assay and the fluorescent treponemal antibody absorption test.
Ijsselmuiden
O E
OE
Meinardi
M M
MM
van der Sluis
J J
JJ
Menke
H E
HE
Stolz
E
E
van Eijk
R V
RV
D000907:Antibodies, Bacterial / Q000032:analysis*; D000942:Antigens, Bacterial / Q000276:immunology; D003101:Collodion; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005374:Filtration; D005455:Fluorescent Antibody Technique; D006386:Hemagglutination Tests; D006801:Humans; D008297:Male; D011237:Predictive Value of Tests; D013588:Syphilis Serodiagnosis; D014210:Treponema pallidum / Q000276:immunology*
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D003101:Collodion