Role of insulin in the stimulation of renal 25-hydroxyvitamin D3-1 alpha-hydroxylase by phosphorus deprivation in rats.
The increase in serum 1,25(OH)2D concentration in response to dietary phosphorus (P) depreviation is dependent on the presence of insulin in rats. The present study was undertaken to clarify whether insulin exerts its effects by affecting the renal production of 1,25(OH)2D. The 25(OH)D-1 alpha-hydroxylase activity in kidney homogenates was markedly stimulated by P deprivation in control rats (0.20 +/- 0.06 pmol/g tissue/min in the rats on a normal P diet v 1.3 +/- 0.15 pmol/g/min in the rats on a low P diet; 6.5-fold increase). In contrast, in streptozotocin-diabetic rats, the increase in the renal 1 alpha-hydroxylase activity in response to P deprivation (0.25 +/- 0.01 pmol/g/min; 3.6-fold increase) as well as the enzyme activity in the rats on a normal P diet (0.07 +/- 0.01 pmol/g/min) was markedly suppressed. Furthermore, all the changes in the renal 1 alpha-hydroxylase activity in insulin-deficient rats disappeared by insulin replacement (0.16 +/- 0.01 pmol/g/min in the rats on a normal P diet v 1.3 +/- 0.01 pmol/g/min in the rats on a low P diet; eightfold increase). These results demonstrate that the stimulation of 1 alpha-hydroxylase in response to dietary P deprivation is blunted by insulin deficiency and is fully restored by insulin replacement. It is suggested that insulin, in addition to its direct stimulatory effect on 1 alpha-hydroxylase, alters the responsiveness of renal 1 alpha-hydroxylase to P deprivation. These effects of insulin on 1 alpha-hydroxylase may be responsible for the change in serum 1,25(OH)2D concentration in response to dietary P deprivation, although the possibility cannot be ruled out that insulin also affects the metabolic clearance of 1,25(OH)2D.
Ikeda
K
K
Matsumoto
T
T
Morita
K
K
Kawanobe
Y
Y
Ezawa
I
I
Ogata
E
E
D000818:Animals; D002117:Calcitriol / Q000097:blood; D002118:Calcium / Q000097:blood; D053493:Cholestanetriol 26-Monooxygenase; D003921:Diabetes Mellitus, Experimental / Q000097:blood; D007328:Insulin / Q000502:physiology*; D007668:Kidney / Q000201:enzymology*; D008297:Male; D010758:Phosphorus / Q000172:deficiency*; D051381:Rats; D011919:Rats, Inbred Strains; D013250:Steroid Hydroxylases / Q000378:metabolism*
D007328:Insulin; D010758:Phosphorus; D013250:Steroid Hydroxylases; D053493:Cholestanetriol 26-Monooxygenase; D002117:Calcitriol; D002118:Calcium