title: Protein phosphorylation and dynamics of cytoskeletal structures associated with basal bodies in Paramecium.
abstract: The presence of phosphorylated proteins associated with microtubule organizing centers in tissue culture cells during mitosis has been demonstrated by the use of monoclonal antibodies raised against mitotic HeLa cells [Vandre et al., Proc. Natl. Acad. Sci. U.S.A. 81:4439-4443, 1984]. We report here that in Paramecium two of the mitosis specific antibodies, MPM-1 and MPM-2, decorate throughout the cell cycle all the microtubule organizing centers (MTOCs) located in the cortex and in the oral apparatus (gullet). Immuno-electron microscopy showed that these antibodies labeled the electron-dense material surrounding basal bodies from which several microtubule networks as well as kinetodesmal fibers originate. During mitosis, these antibodies also stained other cortical cytoskeletal structures, the kinetodesmal fibers (MPM-1 and MPM-2) and the epiplasm (MPM-1). Among the different polypeptides recognized by the antibodies on immunoblots, three major ones of 60, 63, and 116 kDa were found to be common to the cortex (where several thousand ciliary basal bodies are anchored) and the oral apparatus (which comprises several hundred basal bodies around which various arrays of cytoplasmic microtubules are organized). Alkaline phosphatase treatment abolished the immunoreactivity of the polypeptides and the labeling observed by immunofluorescence. These results demonstrate that phosphorylated proteins are associated with all the known active microtubule organizing centers present in the cortex throughout the cell cycle of Paramecium. Furthermore they indicate that in Paramecium phosphorylation of proteins could also be involved in the cell cycle dependent dynamics of cortical cytoskeletal structures other than microtubules.
authors.affiliation: Centre de Génétique Moléculaire, Centre National de la Recherche Scientique, Gif-sur-Yvette, France.
authors.forename: G
authors.initials: G
authors.lastname: Keryer
authors.forename: F M
authors.initials: FM
authors.lastname: Davis
authors.forename: P N
authors.initials: PN
authors.lastname: Rao
authors.forename: J
authors.initials: J
authors.lastname: Beisson
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D003599:Cytoskeleton / Q000648:ultrastructure*; D005455:Fluorescent Antibody Technique; D007399:Interphase; D008854:Microscopy, Electron; D008868:Microtubule Proteins / Q000032:analysis*; D008938:Mitosis; D010247:Paramecium / Q000166:cytology* / Q000648:ultrastructure; D010455:Peptides / Q000032:analysis; D010750:Phosphoproteins / Q000032:analysis*; D010766:Phosphorylation
chemical_list: D000911:Antibodies, Monoclonal; D008868:Microtubule Proteins; D010455:Peptides; D010750:Phosphoproteins