[Development of an immunoenzyme test system for determining the antigens of feed yeasts].
An enzyme immunoassay (EIA) system for the detection of fodder yeast antigens in the air of production areas at fodder protein producing plants has been developed. The method has proved to be highly sensitive and specific and shows advantages in comparison with the nonspecific method of low sensitivity, currently used at such plants. The sensitivity of solid-phase EIA techniques is 0.001 micrograms/ml (for protein) or 10(2)-10(3) cells/ml, and 10 ng/ml for soluble antigen. No cross reactions with bakers' yeast antigen have been observed.
Pavlova
I P
IP
Kleeva
O B
OB
D000395:Air Pollutants, Occupational / Q000032:analysis; D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D000946:Antigens, Fungal / Q000032:analysis*; D002138:Calibration; D005069:Evaluation Studies as Topic; D007106:Immune Sera / Q000302:isolation & purification; D007114:Immunization; D007124:Immunoenzyme Techniques / Q000295:instrumentation*; D011817:Rabbits; D012995:Solubility; D015002:Yeast, Dried / Q000276:immunology*
D000395:Air Pollutants, Occupational; D000946:Antigens, Fungal; D007106:Immune Sera
Razrabotka immunofermentnoĭ test-sistemy dlia opredeleniia antigenov kormovykh drozhzheĭ.