Occurrence of anti-gizzard P34K antibody cross-reactive components in bovine smooth muscles and non-smooth muscle tissues.
In our previous paper (Biochem. Biophys. Res. Commun. 141, 20-26 (1986) we reported the isolation of a 34000-dalton protein (p34K) which binds to calmodulin and F-actin from chicken gizzard smooth muscle. We examined the distribution of the immunoreactive component of gizzard p34K in bovine tissues by immunoblot analysis using a rabbit polyclonal antibody raised against gizzard p34K. The immunoreactive components with molecular weights of 33000-35000 were detected in all smooth muscles from aorta, esophagus, stomach, trachea and uterus. In non-smooth muscle tissues, a 36000-dalton cross-reactive protein was present in adrenal medulla and cortex. The immunoreactive form of gizzard p34K occurred in large amounts in smooth muscles from various bovine tissues.
Takahashi
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Hiwada
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Kokubu
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D000818:Animals; D000906:Antibodies; D002417:Cattle; D002645:Chickens; D005895:Gizzard, Avian / Q000032:analysis; D007124:Immunoenzyme Techniques; D008970:Molecular Weight; D009124:Muscle Proteins / Q000032:analysis*; D009130:Muscle, Smooth / Q000032:analysis*; D011506:Proteins / Q000032:analysis*; D014018:Tissue Distribution
D000906:Antibodies; D009124:Muscle Proteins; D011506:Proteins; C053028:gizzard p34K protein, chicken