[Quantitative detection and elimination of neuroblastoma cells in vitro. Comparative study of the efficacy of an immunomagnetic method and lymphokine-activated killer cells].
Much effort is devoted to eliminating residual tumor cells - which escape detection by conventional methods - from remission bone marrow harvested for autologous transplantation. The quantitative determination of the efficacy of these purging methods is generally difficult. This report describes an in vitro reproducible tumor model. Normal mononuclear blood cells were deliberately contaminated with cells from the human neuroblastoma cell line SK-N-AS. The frequency of clonogenic SK-N-AS cells was determined in limiting dilution culture before and after treatment. Two methods of purging these tumor cells from the mixed cell suspension were compared, 1) an immunomagnetic method taking advantage of the existence of a monoclonal antibody (mAb) specific for 90-95% of the tumor cells, and 2) a method based on cell mediated cytotoxicity testing the activity of previously lymphokine activated killer (LAK) cells. Immunomagnetic purging eliminated 90% (1 log) of all SK-N-AS cells and 99% (2 log) of the mAb-binding clonogenic tumor cells. In contrast, LAK cell treatment removed only between 0.1 to 0.3 log (23-50%) of the clonogenic SK-N-AS cells.
Kästli
M
M
Division d'immunologie et d'allergologie, Hôpital cantonal universitaire, Genève.
Aapro
M
M
Blaser
K
K
Jeannet
M
M
Irlé
C
C
D000911:Antibodies, Monoclonal; D016026:Bone Marrow Transplantation; D002460:Cell Line; D003114:Colony-Forming Units Assay; D003470:Culture Media; D006801:Humans; D007376:Interleukin-2; D007694:Killer Cells, Natural; D008280:Magnetics; D008863:Microspheres; D009447:Neuroblastoma / Q000473:pathology; D014410:Tumor Stem Cell Assay
D000911:Antibodies, Monoclonal; D003470:Culture Media; D007376:Interleukin-2
Détection quantitative et élimination des cellules de neuroblastome in vitro.Etude comparative ente l'efficacité d'une méthode immunomagnetique et celle des cellules LAK.