title: Reduced adherence of micro-organisms to human mucosal epithelial cells following treatment with Taurolin, a novel antimicrobial agent.
abstract: Taurolin, a non-antibiotic antimicrobial agent, significantly reduced the adherence of buccal and vaginal strains of Candida albicans blastospores and urine isolates of Escherichia coli and Staphylococcus saprophyticus to epithelial cells. Light microscopy and radio-isotopic counting methods were used to quantify the adherence of the micro-organisms to either uroepithelial or buccal epithelial cells. A maximum reduction in adherence of approximately 65% was obtained. The anti-adherence capacity was time-dependent, requiring a contact time of 30 min to achieve maximum effect. Taurolin at sub-minimum inhibitory concentrations (MIC) significantly reduced the adherence of Candida and E. coli. A concentration slightly higher than the MIC was required for Staph. saprophyticus. Treatment of either epithelial cells or micro-organisms with Taurolin resulted in reduced adherence of microorganisms.
authors.forename: S P
authors.initials: SP
authors.lastname: Gorman
authors.forename: D F
authors.initials: DF
authors.lastname: McCafferty
authors.forename: A D
authors.initials: AD
authors.lastname: Woolfson
authors.forename: D S
authors.initials: DS
authors.lastname: Jones
mesh_terms: D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents / Q000494:pharmacology*; D001422:Bacterial Adhesion / Q000187:drug effects; D002176:Candida albicans / Q000187:drug effects* / Q000502:physiology; D002448:Cell Adhesion; D004847:Epithelial Cells; D004848:Epithelium / Q000382:microbiology; D004926:Escherichia coli / Q000187:drug effects* / Q000502:physiology; D005260:Female; D006801:Humans; D008297:Male; D009061:Mouth Mucosa / Q000382:microbiology; D013210:Staphylococcus / Q000187:drug effects* / Q000502:physiology; D013654:Taurine / Q000031:analogs & derivatives*; D013829:Thiadiazines / Q000494:pharmacology*; D013843:Thiazines / Q000494:pharmacology*; D014551:Urinary Tract / Q000382:microbiology; D014556:Urine / Q000166:cytology
chemical_list: D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D013829:Thiadiazines; D013843:Thiazines; D013654:Taurine; C012566:taurolidine