Role of Mg2+ in the ribozyme system.
Recently, several kinds of catalytic activities have been identified from L-19 IVS RNA or 'ribozyme'. In the catalytic process of ribozyme, Mg2+ is required. We investigated the role of Mg2+ by molecular orbital calculation. Based on the results of the calculation, a model for the self-splicing reaction was constructed.
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Imamura
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D000818:Animals; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology*; D012326:RNA Splicing / Q000187:drug effects*; D016337:RNA, Catalytic; D012335:RNA, Ribosomal / Q000187:drug effects / Q000378:metabolism*; D013768:Tetrahymena / Q000235:genetics
D016337:RNA, Catalytic; D012335:RNA, Ribosomal; D008274:Magnesium