Partial assignment of disulfide pairs in neurophysins.
The original report assigning the pairing of neurophysin's 14 half-cystine residues (Schlesinger et al. (1972), Proc. Natl. Acad. Sci., U.S.A., 69,3350-3353) was based on an incorrect amino acid sequence. In the present study, re-investigation of the results of proteolytic fragmentation of bovine neurophysins indicates that the majority of the original assignments were incorrect. Three disulfide pairs are now assigned as Cys21-Cys44, Cys67-Cys85 and Cys74-Cys79. The pairing pattern indicates that neurophysin's variable carboxyl terminal region, separately encoded by the third gene exon, does not form a self-contained domain.
Burman
S
S
Department of Biochemistry, Cornell University Medical College, New York.
Breslow
E
E
Chait
B T
BT
Chaudhary
T
T
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D003553:Cystine; D004220:Disulfides / Q000032:analysis; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009481:Neurophysins; D010447:Peptide Hydrolases; D014357:Trypsin
D004220:Disulfides; D009481:Neurophysins; D003553:Cystine; D010447:Peptide Hydrolases; D014357:Trypsin