Synthesis and reactivity of intermediates formed in the T4 RNA ligase reaction.
The intermediate adenylated donor derivatives A(5')pp(5')dTp and A(5')pp(5')GpGpGp have been prepared from suitable phosphorylating reagents activated by 1-hydroxybenzotriazole. Phosphodiester bond formation between donor and acceptor oligonucleotides as catalyzed by T4 RNA ligase is shown to be more efficient when the adenylated form of the donor molecule is used.
Hoffmann
P U
PU
McLaughlin
L W
LW
D004926:Escherichia coli / Q000201:enzymology*; D007202:Indicators and Reagents; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis*; D011118:Polynucleotide Ligases / Q000378:metabolism*; D011130:RNA Ligase (ATP) / Q000378:metabolism*; D013379:Substrate Specificity; D013604:T-Phages / Q000201:enzymology*
D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides; D011118:Polynucleotide Ligases; D011130:RNA Ligase (ATP)