[Production of monoclonal antibody against a newly established human endometrial cancer cell line SNG-II].
A cell line designated SNG-II was established from the operation specimen of human endometrial adenocarcinoma, and by means of an immunization procedure using intact SNG-II cells, a monoclonal antibody (Mab) named MSN-1 which reacts immunohistochemically with endometrial cancers was obtained. The cell line grew well without interruption for over 5 years, and, SNG-II cells produced tumors of cell differentiated adenocarcinoma in nude mice. The modal chromosomal number was diploid without a marker chromosome. The production of human chorionic gonadotropin and its beta-subunit, CA-125, tissue polypeptide antigen, and placental proteins such as PP6 and PP7 in SNG-II was confirmed. MSN-1 was of IgM subclass. As the antigenic reactivity was unchanged by trypsin treatment, but lost by periodic treatment, it was suggested that the antigen corresponding to MSN-1 was a carbohydrate sequence. Immunohistochemically MSN-1 reacted with about 70% cases of endometrial adenocarcinoma, but seldom with normal endometrium. Furthermore, the staining pattern of MSN-1 was different in benign cells from that in malignant cells: only the luminal surface of the normal endometrium was positive, whereas the cytoplasma was also stained in many of adenocarcinoma cells.
Nozawa
S
S
Sakayori
M
M
Takayama
Y
Y
Tsukazaki
K
K
Aoki
D
D
Iizuka
R
R
Mukai
M
M
Inaba
N
N
D000230:Adenocarcinoma / Q000276:immunology* / Q000473:pathology; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000951:Antigens, Neoplasm / Q000032:analysis; D002460:Cell Line; D002874:Chromosome Mapping; D005260:Female; D006801:Humans; D006825:Hybridomas; D007124:Immunoenzyme Techniques; D051379:Mice; D014594:Uterine Neoplasms / Q000276:immunology* / Q000473:pathology
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm