The mechanism of 2-deoxy-glucose-induced insulin secretion in the mouse.
1 Intravenous injection of 2-deoxy-glucose (3 mmol/kg) increases plasma levels of insulin and glucose in the mouse. This study investigated the contribution of various autonomic receptors to the plasma insulin response. 2 Pretreatment with the cholinergic antagonist methylatropine abolished the response completely. 3 Pretreatment with the alpha-adrenoceptor antagonist phentolamine caused a potentiated response, whereas the beta-adrenoceptor antagonist L-propranolol had no effect. 4 Pretreatment with methylatropine and phentolamine combined caused a small insulin response, which was inhibited by propranolol. 5 Pretreatment with the nicotonic antagonist hexamethonium abolished the response. 6 It is concluded that 2-deoxy-glucose induces a stimulation of insulin secretion in vivo in the mouse predominantly by a cholinergic mechanism. 7 2-Deoxy-glucose also activates alpha-adrenergic mechanisms which affect the size of the insulin secretory response. beta-Adrenoceptor and non-adrenergic, non-cholinergic mechanisms seem to be less important.
Karlsson
S
S
Department of Surgery, Lund University, Sweden.
Bood
M
M
Ahrén
B
B
D000818:Animals; D001286:Atropine Derivatives / Q000494:pharmacology; D001786:Blood Glucose / Q000378:metabolism; D003837:Deoxy Sugars / Q000494:pharmacology*; D003847:Deoxyglucose / Q000494:pharmacology*; D005260:Female; D006584:Hexamethonium Compounds / Q000494:pharmacology; D007328:Insulin / Q000097:blood / Q000378:metabolism*; D000078790:Insulin Secretion; D051379:Mice; D010646:Phentolamine / Q000494:pharmacology; D011433:Propranolol / Q000494:pharmacology*; D011941:Receptors, Adrenergic / Q000187:drug effects
D001286:Atropine Derivatives; D001786:Blood Glucose; D003837:Deoxy Sugars; D006584:Hexamethonium Compounds; D007328:Insulin; D011941:Receptors, Adrenergic; C006649:methylatropine; D003847:Deoxyglucose; D011433:Propranolol; D010646:Phentolamine