Response of diabetic basement membrane--producing cells to glucose and insulin.
The effect of insulin and glucose on the synthesis of basement membrane components was studied in organ cultures of a basement membrane-producing tumor grown in diabetic and normal mice. Tumor tissue grown in diabetic mice produced more protein and basement membrane-specific proteoglycan in response to insulin than tissue grown in normal mice. Addition of high levels of glucose to the culture medium did not alter insulin-stimulated protein synthesis by diabetic or normal tissue but dampened insulin-stimulated production of proteoglycan. These data suggest that basement membrane-producing cells in diabetic hosts may be hypersensitive to insulin and that stimulation of protein production by insulin may play some role in the in situ hypertrophy of basement membranes.
Ledbetter
S R
SR
Wagner
C W
CW
Martin
G R
GR
Rohrbach
D H
DH
Hassell
J R
JR
D000818:Animals; D001485:Basement Membrane / Q000187:drug effects* / Q000378:metabolism; D002478:Cells, Cultured; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D005947:Glucose / Q000494:pharmacology*; D007328:Insulin / Q000494:pharmacology*; D051379:Mice; D008810:Mice, Inbred C57BL; D014176:Protein Biosynthesis; D011509:Proteoglycans / Q000096:biosynthesis
D007328:Insulin; D011509:Proteoglycans; D005947:Glucose