Selection against expression of the Escherichia coli gene gpt in hprt+ mouse teratocarcinoma and hybrid cells.
Thioxanthine is toxic for mammalian cells transformed by the dominant selectable marker gpt. It allowed us to select, in the presence of the endogenous hypoxanthine-guanine phosphoribosyltransferase gene, mutants that did not express gpt any more and also hybrid cells that had lost the chromosome carrying it. The gpt marker is thus dominant in negative as well as in positive selection, which makes it potentially very useful for genetic studies of mammalian cells.
Besnard
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C
Institut Jacques Monod, Unité 257 de I'Institut National de la Santé et de la Recherche Médicale, Université Paris VII, France.
Monthioux
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E
Jami
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J
D000818:Animals; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005796:Genes; D005798:Genes, Bacterial; D005799:Genes, Dominant; D006822:Hybrid Cells / Q000166:cytology* / Q000201:enzymology; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics*; D051379:Mice; D009154:Mutation; D010430:Pentosyltransferases / Q000235:genetics*; D013724:Teratoma / Q000201:enzymology / Q000235:genetics*; D014158:Transcription, Genetic; D014970:Xanthines / Q000633:toxicity
D014970:Xanthines; C054700:6-thioxanthine; D010430:Pentosyltransferases; C024827:xanthine phosphoribosyltransferase; D007041:Hypoxanthine Phosphoribosyltransferase