Ile-Ser-bradykinin (T-kinin) and Met-Ile-Ser-bradykinin (Met-T-kinin) are released from T-kininogen by an acid proteinase of granulomatous tissues in rats.
An acid proteinase of granulomatous tissues in rats with carrageenin-induced inflammation released kinin from T-kininogen. The kinin isolated by n-butanol extraction was separated by reverse-phase high-performance liquid chromatography into T-kinin and a T-kinin derivative. From determination of its amino acid composition and its immunoreactivity toward anti-bradykinin antiserum, the T-kinin derivative was identified as Met-Ile-Ser-bradykinin (Met-T-kinin).
Sakamoto
W
W
Satoh
F
F
Gotoh
K
K
Uehara
S
S
D000818:Animals; D016282:Aspartic Acid Endopeptidases; D001920:Bradykinin / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000378:metabolism; D002351:Carrageenan; D002851:Chromatography, High Pressure Liquid; D010450:Endopeptidases / Q000378:metabolism*; D006099:Granuloma / Q000201:enzymology*; D007249:Inflammation; D007704:Kininogens / Q000378:metabolism*; D051381:Rats
D007704:Kininogens; C053343:bradykinin, Met-Ile-Ser-; C039065:T-kinin; D002351:Carrageenan; D010450:Endopeptidases; D016282:Aspartic Acid Endopeptidases; D001920:Bradykinin