Nucleotide sequence of the Klebsiella pneumoniae nifD gene and predicted amino acid sequence of the alpha-subunit of nitrogenase MoFe protein.
The nucleotide sequence of the Klebsiella pneumoniae nifD gene is presented and together with the accompanying paper [Holland, Zilberstein, Zamir & Sussman (1987) Biochem. J. 247, 277-285] completes the sequence of the nifHDK genes encoding the nitrogenase polypeptides. The K. pneumoniae nifD gene encodes the 483-amino acid-residue nitrogenase alpha-subunit polypeptide of Mr 54156. The alpha-subunit has five strongly conserved cysteine residues at positions 63, 89, 155, 184 and 275, some occurring in a region showing both primary sequence and potential structural homology to the K. pneumoniae nitrogenase beta-subunit. A comparison with six other alpha-subunit amino acid sequences has been made, which indicates a number of potentially important domains within alpha-subunits.
Ioannidis
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A.F.R.C. Unit of Nitrogen Fixation, University of Sussex, Brighton, U.K.
Buck
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M
D000595:Amino Acid Sequence; D001483:Base Sequence; D003545:Cysteine / Q000032:analysis; D005288:Ferredoxins / Q000032:analysis*; D005798:Genes, Bacterial; D007711:Klebsiella pneumoniae / Q000201:enzymology* / Q000235:genetics; D008969:Molecular Sequence Data; D008983:Molybdoferredoxin / Q000032:analysis*; D009586:Nitrogen Fixation / Q000235:genetics*; D009591:Nitrogenase / Q000235:genetics*; D013045:Species Specificity
D005288:Ferredoxins; D008983:Molybdoferredoxin; D009591:Nitrogenase; D003545:Cysteine