title: Monoclonal antibody against a sperm antigen Mr 95,000 inhibits attachment of human spermatozoa to the zona pellucida.
abstract: A murine monoclonal antibody raised against hamster spermatozoa was found to cross-react with human spermatozoa. By immunofluorescence, the antigen was visualized over the equatorial segment of human sperm heads. In the presence of antibody, sperm binding to the zona pellucida of salt-stored human oocytes was significantly inhibited (P less than or equal to 0.005) compared with other antibodies or control preparations. Using SDS-PAGE of whole spermatozoa and membrane preparations followed by Western blot analysis, the antigen was identified as a determinant with a relative molecular weight of 95,000.
authors.affiliation: MRC/AFRC Comparative Physiology Research Group, Zoological Society of London, U.K.
authors.forename: H D
authors.initials: HD
authors.lastname: Moore
authors.forename: T D
authors.initials: TD
authors.lastname: Hartman
authors.forename: A P
authors.initials: AP
authors.lastname: Bye
authors.forename: P
authors.initials: P
authors.lastname: Lutjen
authors.forename: M
authors.initials: M
authors.lastname: De Witt
authors.forename: A O
authors.initials: AO
authors.lastname: Trounson
mesh_terms: D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D000941:Antigens / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D008297:Male; D008970:Molecular Weight; D010063:Ovum / Q000502:physiology*; D013084:Sperm-Ovum Interactions; D013094:Spermatozoa / Q000166:cytology / Q000276:immunology* / Q000502:physiology; D015044:Zona Pellucida / Q000502:physiology* / Q000648:ultrastructure
chemical_list: D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D000941:Antigens