Characterization of protein kinase C from normal and transformed cultured murine fibroblasts.
Protein kinase C of normal and ras-transformed NIH 3T3 cells was purified by chromatography on TSK DEAE-5PW, threonine-Sepharose, and TSK phenyl-5PW columns. Comparison of the fibroblast enzyme with several types of rat brain protein kinase C by chromatography on a hydroxyapatite column and by immunoblotting, indicates that both normal and transformed fibroblasts possess only one of the four subspecies of protein kinase C which have been identified in brain tissues. This subspecies presumably has the structure encoded by alpha-sequence or a closely related sequence. No significant difference was seen between those enzymes purified from normal and transformed fibroblasts.
McCaffrey
P G
PG
Rosner
M R
MR
Kikkawa
U
U
Sekiguchi
K
K
Ogita
K
K
Ase
K
K
Nishizuka
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D001921:Brain / Q000201:enzymology; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D002850:Chromatography, Gel; D005347:Fibroblasts / Q000201:enzymology*; D007163:Immunosorbent Techniques; D051379:Mice; D009857:Oncogenes; D011493:Protein Kinase C / Q000378:metabolism*; D051381:Rats
D000911:Antibodies, Monoclonal; D011493:Protein Kinase C