Tumor cell growth fraction in Hodgkin's disease.
The growth fraction of tumor cells was studied in 45 cases of Hodgkin's disease by means of a recently developed double immunostaining technique using monoclonal antibody Ki-1, which reacts selectively with Hodgkin and Reed-Sternberg cells in tissues affected by Hodgkin's disease, and antibody Ki-67, which recognizes a cell proliferation-associated nuclear antigen. The medians of the growth fractions of the tumor cells in all histologic subtypes of Hodgkin's disease varied between 78% and 83%. In none of the cases investigated did we find a growth fraction below 50%. Furthermore, mononucleated Hodgkin cells as well as multi-nucleated Reed-Sternberg cells showed a similar Ki-67 labeling index, indicating that both tumor cell types belong to the proliferating pool of this malignancy.
Gerdes
J
J
Van Baarlen
J
J
Pileri
S
S
Schwarting
R
R
Van Unnik
J A
JA
Stein
H
H
D000911:Antibodies, Monoclonal; D000941:Antigens / Q000276:immunology; D002455:Cell Division; D002467:Cell Nucleus / Q000276:immunology; D006651:Histocytochemistry; D006689:Hodgkin Disease / Q000473:pathology*; D006801:Humans; D007124:Immunoenzyme Techniques
D000911:Antibodies, Monoclonal; D000941:Antigens