[Protease activity of the nuclear matrix of rat hepatocytes].
It was demonstrated that the nuclear matrix of rat liver possesses the protease activity. The specific activity of nuclear matrix proteases exceeds that of intact nuclei 7-fold. The optimum activity of nuclear matrix proteases is observed at pH 8-9. The protease activity of the nuclear matrix is inhibited by p-chloromercuribenzoate, N-ethylmaleimide, EDTA, phenylmethylsulfonyl fluoride. This suggests that thiol, serine and metalloproteases are associated with the nuclear matrix.
Kutsyĭ
M P
MP
Malakhova
L V
LV
Gaziev
A I
AI
D000818:Animals; D002467:Cell Nucleus / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008297:Male; D010447:Peptide Hydrolases / Q000378:metabolism*; D011480:Protease Inhibitors; D051381:Rats
D011480:Protease Inhibitors; D010447:Peptide Hydrolases
Proteaznaia aktivnost' iadernogo matriksa gepatotsitov krys.