[Determination of the binding constants of immune serum antibodies to human IgG].
The possibility of using two variants of the enzyme-linked fluorescent cofactor immunoassay for the determination of antibody binding constants has been demonstrated. The determination of binding constants for antibodies isolated by affinity chromatography techniques has been carried out. These techniques permit the isolation of fractions, differing in their affinity by 5-10 times, from the whole population of antibodies.
Shkurina
E A
EA
Rubtsova
M Iu
MIu
Gavrilova
E M
EM
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D000915:Antibody Affinity; D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D002846:Chromatography, Affinity / Q000379:methods; D005455:Fluorescent Antibody Technique; D006801:Humans; D007106:Immune Sera / Q000276:immunology*; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000378:metabolism*; D011817:Rabbits
D007106:Immune Sera; D007074:Immunoglobulin G; D000255:Adenosine Triphosphate
Opredelenie konstant sviazyvaniia antitel immunnykh syvorotok k IgG cheloveka.