title: Directional cDNA cloning in plasmid vectors by sequential addition of oligonucleotide linkers.
authors.forename: D M
authors.initials: DM
authors.lastname: Helfman
authors.forename: J C
authors.initials: JC
authors.lastname: Fiddes
authors.forename: D
authors.initials: D
authors.lastname: Hanahan
mesh_terms: D003001:Cloning, Molecular / Q000379:methods*; D004247:DNA / Q000378:metabolism*; D004256:DNA Polymerase I / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D005822:Genetic Vectors; D009841:Oligonucleotides / Q000138:chemical synthesis; D010957:Plasmids
chemical_list: D009841:Oligonucleotides; D004247:DNA; D004256:DNA Polymerase I