Evidence for intermediate filaments in squirrelfish erythrophores of Holocentrus ascensionus (Rufus).
We have documented the presence of intermediate filaments (IF) in cultured erythrophores of the squirrelfish Holocentrus ascensionus (Rufus). SDS-PAGE and Western blots with monoclonal antibodies T11 and R12 demonstrated that isolated IF consisted of a pair of polypeptides of 54 and 52 kDa. Immunofluorescent studies revealed that the two proteins formed prominent radially oriented IF networks in erythrophores. Immunoelectron microscopic studies showed that the IF were distributed in a "spider-web"-like network of filaments which occasionally intersected with the microtubule surfaces. The IF proteins also were found in fish iridiphores but not in fish epithelial cells which cocultured with the chromatophores.
Stearns
M E
ME
Department of Pharmacology, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111.
Wang
M
M
D000818:Animals; D001692:Biological Transport; D002856:Chromatophores / Q000032:analysis* / Q000276:immunology; D003599:Cytoskeleton / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D005399:Fishes; D005455:Fluorescent Antibody Technique; D007381:Intermediate Filament Proteins / Q000032:analysis; D007382:Intermediate Filaments / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D010860:Pigments, Biological / Q000378:metabolism; D012967:Sodium Dodecyl Sulfate
D007381:Intermediate Filament Proteins; D010860:Pigments, Biological; D012967:Sodium Dodecyl Sulfate