title: Lysosomal enzyme pattern in lung lymph and blood during E. coli sepsis in sheep.
abstract: Systemic release of lysosomal enzymes and local release in the pulmonary microcirculation from sequestrated and activated leucocytes could be an important factor in the development of the lung microvascular injury seen after septicaemia. The maximal activities of 11 lysosomal acid hydrolases (acid phosphatase, alpha- and beta-glucosidase, alpha- and beta-galactosidase, alpha-mannosidase, beta-acetylglucosaminidase, beta-glucuronidase, arylamidase and cathepsins B and C) were measured in serum and lung lymph from seven sheep before and after infusion of live E. coli bacteria. In the early phase of septicaemia (the first hour) the activities of eight enzymes were increased in serum and/or lung lymph (1.1 to 2X pre-infusion values). In the late phase, 3-4 h after sepsis, there were significantly elevated serum activities of beta-glucosidase (5.4X), alpha- and beta-galactosidases (2.7X, 1.5X), beta-acetylglucosaminidase (2.0X) arylamidase (1.2X) and cathespin B (1.7X). In lymph acid phosphatase (1.7X), alpha- and beta-glucosidases (1.6X, 6.4X), alpha- and beta-galactosidases (2.1X, 1.7X). Beta-acetylglucosaminidase (2.6X), and beta-glucuronidase (4.0X pre-infusion) were elevated. The findings of a heterogenicity of changes in serum and lymph activities, as well as the large molecular sizes of some of the enzymes with changed activities indicated to us that permeability changes were not major causes of increased lymph enzyme activities. The results could indicate a local release of enzymes either from sequestrated leucocytes or lung tissue due to local reactions in the lung or lung microvessels. The heterogenous changes in activities for the various lysosomal enzymes as found in the present study indicated that measurement of only one enzyme could be misleading.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.forename: S
authors.initials: S
authors.lastname: Andreasson
authors.forename: L
authors.initials: L
authors.lastname: Smith
authors.forename: O K
authors.initials: OK
authors.lastname: Andersen
authors.forename: G
authors.initials: G
authors.lastname: Volden
authors.forename: B
authors.initials: B
authors.lastname: Risberg
mesh_terms: D000818:Animals; D004927:Escherichia coli Infections / Q000201:enzymology*; D006867:Hydrolases / Q000378:metabolism*; D008168:Lung / Q000098:blood supply / Q000201:enzymology*; D008196:Lymph / Q000201:enzymology*; D008247:Lysosomes / Q000201:enzymology*; D008833:Microcirculation; D018805:Sepsis / Q000201:enzymology*; D012756:Sheep
chemical_list: D006867:Hydrolases