title: Clonal immunoglobulin gene rearrangements in tissues involved by Hodgkin's disease.
abstract: The nature of Reed-Sternberg cells, the abnormal cells of Hodgkin's disease, is controversial. Morphological and immunologic marker studies suggested different cells of origin. To investigate a possible B or T cell origin, immunoglobulin and T cell receptor gene analyses were performed on tissues from 11 patients in early and late stages of Hodgkin's disease. In addition, the immunologic marker patterns of the Reed-Sternberg cells were determined. Rearrangements of immunoglobulin heavy- and light-chain genes were detected in tissues from five patients, particularly in late stages of the disease when lymphocyte depletion had occurred. No rearrangements of T cell receptor genes were found. The results indicate that clonal immunoglobulin gene rearrangements can be detected in tissues involved by Hodgkin's disease.
authors.forename: M G
authors.initials: MG
authors.lastname: Brinker
authors.forename: S
authors.initials: S
authors.lastname: Poppema
authors.forename: C H
authors.initials: CH
authors.lastname: Buys
authors.forename: W
authors.initials: W
authors.lastname: Timens
authors.forename: J
authors.initials: J
authors.lastname: Osinga
authors.forename: L
authors.initials: L
authors.lastname: Visser
mesh_terms: D000293:Adolescent; D000328:Adult; D000954:Antigens, Surface / Q000032:analysis; D002869:Chromosome Aberrations; D004247:DNA / Q000378:metabolism; D005260:Female; D005796:Genes; D006644:Histiocytes / Q000473:pathology; D006651:Histocytochemistry; D006689:Hodgkin Disease / Q000473:pathology*; D006801:Humans; D007124:Immunoenzyme Techniques; D007136:Immunoglobulins / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009693:Nucleic Acid Hybridization; D011948:Receptors, Antigen, T-Cell / Q000235:genetics; D014018:Tissue Distribution
chemical_list: D000954:Antigens, Surface; D007136:Immunoglobulins; D011948:Receptors, Antigen, T-Cell; D004247:DNA