title: Slide immunoenzymatic assay (SIA): improving sensitivity to measure antibodies when samples are very small and dilute, and antigen is scarce.
abstract: Modifications to the slide immunoenzymatic assay (SIA) using the PAP reagent (SIA-PAP) were developed which increase sensitivity considerably. These SIA modifications are particularly useful for measuring speedily antibodies in dilute samples available only in microliter-volumes when antigen is scarce, whether a molecule, or a prokaryotic or eukaryotic cell.
authors.affiliation: Wadsworth Center for Laboratories and Research, New York State Department of Health, Albany.
authors.forename: E
authors.initials: E
authors.lastname: Conway de Macario
authors.forename: R J
authors.initials: RJ
authors.lastname: Jovell
authors.forename: A J
authors.initials: AJ
authors.lastname: Macario
mesh_terms: D000906:Antibodies / Q000032:analysis; D000937:Antigen-Antibody Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D007124:Immunoenzyme Techniques
chemical_list: D000906:Antibodies