[Use of immunoenzyme analysis employing a staphylococcal protein A conjugate with peroxidase in the serodiagnosis of influenza].
A test-system was developed on the basis of solid-phase enzyme-immunoassay using protein A/peroxidase conjugate for the determination of antibody levels to influenza virus in sera of humans who had experienced a natural infection or received a live influenza vaccine. The accurate observation of the test conditions was demonstrated to give the results well correlating with those of the HI test. The use of isolated hemagglutinin as the antigen considerably increased the specificity of the enzyme-immunoassay and in a number of cases detected a 4-fold or higher rise of antibody titres to hemagglutinin in paired sera of the vaccinees where the HI test showed no rise in antibody titres.
Egorenkova
E M
EM
Khristova
M L
ML
Leonov
S V
SV
Kozhukharova
M
M
Demidova
S A
SA
D000293:Adolescent; D000328:Adult; D000914:Antibodies, Viral / Q000032:analysis; D005069:Evaluation Studies as Topic; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D007124:Immunoenzyme Techniques; D009980:Influenza A virus / Q000276:immunology; D007251:Influenza, Human / Q000175:diagnosis*; D008889:Military Personnel; D012698:Serologic Tests / Q000379:methods; D013205:Staphylococcal Protein A
D000914:Antibodies, Viral; D013205:Staphylococcal Protein A
Primenenie immunofermentnogo analiza s ispol'zovaniem kon''iugata belka A stafilokokka s peroksidazoĭ v serodiagnostike grippa.