Light and electron microscopic studies of localized laryngeal amyloidosis.
We performed light and electron microscopic studies in a case of localized supraglottic laryngeal amyloidosis involving a 55-year-old woman. Tissue specimens were stained with hematoxylin and eosin, and alkaline Congo red with or without permanganate (KMnO4) treatment, and were also examined with peroxidase-antiperoxidase immunohistochemistry. Biopsy specimens from the epiglottis showed diffuse replacement of the subepithelial region by amyloid, which showed a typical green birefringence under polarized light. Tissue affinity for Congo red was persistent after the KMnO4 treatment. However, the tissue sections showed a positive reaction only to the anti-P component antibody. Electron microscopic studies revealed some disorders of the plasma cells, suggesting that these cells play an important role in focal production of amyloid fibrils.
Nagata
H
H
Department of Otorhinolaryngology, School of Medicine, Chiba University, Japan.
Yoshihara
T
T
Nomoto
M
M
Kanda
T
T
Kaneko
T
T
Yaku
Y
Y
D000682:Amyloid / Q000032:analysis; D000686:Amyloidosis / Q000276:immunology / Q000473:pathology*; D004825:Epiglottis / Q000648:ultrastructure*; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D007818:Laryngeal Diseases / Q000276:immunology / Q000473:pathology*; D008875:Middle Aged
D000682:Amyloid