The cross-reaction patterns of the MRC OX-3, OX-6, and OX-17 monoclonal antibodies on rat inbred and congenic strains.
MRC OX-3, MRC OX-6, and MRC OX-17 antibodies directed against rat MHC class II antigens were tested on a panel of rat inbred and congenic strains with different haplotypes using indirect immunoperoxidase and immunofluorescence techniques. In accordance with reports of others the MRC OX-17 was found to define RT1 class II monomorphic and the MRC OX-3 polymorphic determinants. MRC OX-6, giving no reaction with RT1d and RT1dv1 haplotypes, was shown to be directed against a rat MHC class II polymorphic determinant.
Otová
B
B
Department of Biology, Faculty of Medicine, Charles University, Praha.
Kren
V
V
Bukovský
A
A
Pospíchalová
J
J
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D003429:Cross Reactions; D005455:Fluorescent Antibody Technique; D006681:HLA-D Antigens / Q000276:immunology*; D006239:Haplotypes; D007124:Immunoenzyme Techniques; D008285:Major Histocompatibility Complex; D051381:Rats; D011919:Rats, Inbred Strains / Q000276:immunology*
D000911:Antibodies, Monoclonal; D006681:HLA-D Antigens