title: Comparison of the properties of the purified beta-glucosidase from the transformed strain of Saccharomyces cerevisiae TYKF2 with that of the donor strain Kluyveromyces fragilis Y610.
abstract: Saccharomyces cerevisiae TYKF2 was obtained by cloning in S. cerevisiae the gene coding for beta-glucosidase in Kluyveromyces fragilis Y610 (ATCC 12424). The beta-glucosidases of both organisms were purified and their biochemical characteristics were determined. The two beta-glucosidases had the same enzymatic properties as those previously described in the literature. The strain S. cerevisiae TYKF2 is able to produce enhanced amounts of enzyme.
authors.affiliation: Chaire de Génétique et Microbiologie, ENSA-INRA, Montpellier, France.
authors.forename: M
authors.initials: M
authors.lastname: Leclerc
authors.forename: P
authors.initials: P
authors.lastname: Chemardin
authors.forename: A
authors.initials: A
authors.lastname: Arnaud
authors.forename: R
authors.initials: R
authors.lastname: Ratomahenina
authors.forename: P
authors.initials: P
authors.lastname: Galzy
authors.forename: C
authors.initials: C
authors.lastname: Gerbaud
authors.forename: A
authors.initials: A
authors.lastname: Raynal
authors.forename: M
authors.initials: M
authors.lastname: Guérineau
mesh_terms: D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D005800:Genes, Fungal; D005959:Glucosidases / Q000235:genetics* / Q000302:isolation & purification; D007716:Kluyveromyces / Q000201:enzymology* / Q000235:genetics; D008970:Molecular Weight; D011489:Protein Denaturation; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics; D004718:Saccharomycetales / Q000201:enzymology*; D013816:Thermodynamics; D014170:Transformation, Genetic
chemical_list: D005959:Glucosidases