Monoclonal anti-K14 and anti-K2.
Mouse hybridoma clones have produced monoclonal antibodies directed against the K:14 and K:2 high-incidence antigens of the Kell blood group system. Two examples of anti-K14 were isolated, each arising from a separate fusion procedure. All three monoclonal antibodies are of the immunoglobulin class IgG1. Serological activity is consistent with that seen with human antibodies to high-incidence Kell system antigens, and their epitopes are destroyed, as usual, by 2-aminoethylisothiuronium bromide treatment. Specificity was further confirmed by adsorption and elution studies. Tests against nonhuman primate red cells demonstrated the expression of K:14 only by the great apes, whereas K:2 was present on all red cells tested. These findings emphasize the usefulness of monoclonal antibodies to elucidate the evolutionary patterns of blood group variants.
Nichols
M E
ME
Rosenfield
R E
RE
Rubinstein
P
P
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D001789:Blood Group Antigens / Q000276:immunology*; D003584:Cytological Techniques; D004912:Erythrocytes / Q000276:immunology; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007136:Immunoglobulins / Q000145:classification; D007626:Kell Blood-Group System / Q000276:immunology*; D011323:Primates
D000911:Antibodies, Monoclonal; D001789:Blood Group Antigens; D007136:Immunoglobulins; D007626:Kell Blood-Group System