title: Glucocorticoid receptor phosphorylation in mouse L-cells.
abstract: This paper summarizes our observations on the phosphorylation state of untransformed and transformed glucocorticoid receptors isolated from 32P-labeled L-cells. The 300-350-kDa 9S untransformed murine glucocorticoid receptor complex is composed of a 100-kDa steroid-binding phosphoprotein and one or possibly two units of the 90-kDa heat shock protein (hsp90), which is also a phosphoprotein. Transformation of this complex to the 4S DNA-binding state is accompanied by dissociation of hsp90. When receptors in cytosol are transformed by heating at 25 degrees C, there is no gross change in the degree of phosphorylation of the steroid-binding protein. Both receptors that are bound to DNA after transformation under cell-free conditions and receptors that are located in the nucleus of cells incubated at 37 degrees C in the presence of glucocorticoid are labeled with 32P. The results of experiments in which the 32P-labeled receptor was submitted to limited proteolysis suggest that the 16-kDa DNA-binding domain is phosphorylated and that the 28-kDa steroid-binding domain is not.
authors.affiliation: Department of Pharmacology, University of Michigan Medical School, Ann Arbor 48109.
authors.forename: E R
authors.initials: ER
authors.lastname: Sanchez
authors.forename: W
authors.initials: W
authors.lastname: Tienrungroj
authors.forename: F C
authors.initials: FC
authors.lastname: Dalman
authors.forename: A L
authors.initials: AL
authors.lastname: Lin
mesh_terms: D000818:Animals; D003600:Cytosol / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000378:metabolism*; D006358:Hot Temperature; D007739:L Cells / Q000378:metabolism*; D051379:Mice; D008954:Models, Biological; D010750:Phosphoproteins / Q000378:metabolism; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism; D011965:Receptors, Glucocorticoid / Q000378:metabolism*
chemical_list: D004268:DNA-Binding Proteins; D006360:Heat-Shock Proteins; D010750:Phosphoproteins; D011965:Receptors, Glucocorticoid; D011494:Protein Kinases