Monoclonal antibodies against human hematopoietic survival and growth factor.
Mouse hybridoma clones were isolated, and found to constitutionally secrete monoclonal antibody (MoAb) immunoglobulins specific for human hematopoietic stem cell growth factor (SCGF). MoAbs were initially screened by dot-blotting and enzyme immunoassay (EIA), and their specificity was confirmed using western blotting and EIA. Protein A-purified MoAb IgG neutralized SCGF activity, namely, granulocyte-macrophage colony potentiating and erythroid burst-promoting activities. The availability of MoAb could extend its application to one-step immunoadsorbent affinity-purification of SCGF as well as analysis on its receptor.
Hiraoka
A
A
Department of Internal Medicine, Osaka Dental University, Japan.
Ohkubo
T
T
Fukuda
M
M
D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000302:isolation & purification*; D000918:Antibody Specificity; D002470:Cell Survival / Q000187:drug effects; D002850:Chromatography, Gel; D003101:Collodion; D004591:Electrophoresis, Polyacrylamide Gel; D006133:Growth Substances / Q000276:immunology*; D016298:Hematopoietic Cell Growth Factors; D006412:Hematopoietic Stem Cells / Q000187:drug effects*; D006801:Humans; D006825:Hybridomas / Q000378:metabolism; D007124:Immunoenzyme Techniques
D000911:Antibodies, Monoclonal; D006133:Growth Substances; D016298:Hematopoietic Cell Growth Factors; D003101:Collodion