Characterization of 2,3-dihydroxybenzoic acid from Nocardia asteroides GUH-2.
Culture filtrates of virulent Nocardia asteroides GUH-2 after growth in acetate minimal medium displayed an absorbance maximum at 320 nm. After isolation by polyamide extraction and anion chromatography, a UV-active compound with this absorbance was shown to be 2,3-dihydroxybenzoic acid (DHB) by nuclear magnetic resonance, gas chromatographic, and mass spectrometric techniques. DHB production under several culture conditions was quantified by a standard high-pressure liquid chromatography assay. Under iron deficiency conditions, N. asteroides GUH-2 excreted up to 11 mg of DHB per liter into the culture medium. No DHB was detected when N. asteroides GUH-2 was grown in an iron-rich medium. With the less virulent strain N. asteroides 10905, DHB was not found under any condition tested.
Feistner
G J
GJ
Beaman
B L
BL
D000327:Adsorption; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D003470:Culture Media; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D062385:Hydroxybenzoates / Q000032:analysis* / Q000378:metabolism; D007501:Iron / Q000378:metabolism; D007502:Iron Chelating Agents / Q000032:analysis* / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D009616:Nocardia asteroides / Q000378:metabolism* / Q000472:pathogenicity; D009757:Nylons / Q000378:metabolism; D014774:Virulence
D003470:Culture Media; D062385:Hydroxybenzoates; D007502:Iron Chelating Agents; D009757:Nylons; C009135:2,3-dihydroxybenzoic acid; D007501:Iron