Rapid detection of influenza virus in cell culture by indirect immunoperoxidase staining with type-specific monoclonal antibodies.
Seventy respiratory specimens culture-positive for influenza virus were stored at -70 degrees C and retested in duplicate by virus isolation and by indirect immunoperoxidase (IPA) staining of cell monolayers 24 hr postinoculation using influenza type-specific monoclonal antibodies. The IPA stain was positive for 24 of 28 specimens from which influenza virus was reisolated and for eight specimens from which influenza virus was not reisolated.
Swenson
P D
PD
North Shore University Hospital, Department of Medicine, Manhasset, New York.
Kaplan
M H
MH
D000818:Animals; D000911:Antibodies, Monoclonal; D002478:Cells, Cultured; D006801:Humans; D007124:Immunoenzyme Techniques; D009975:Orthomyxoviridae / Q000302:isolation & purification*
D000911:Antibodies, Monoclonal