Facile preparation of purine and pyrimidine 2-deoxy-beta-D-ribonucleosides by biotransformation on encapsulated cells.
A preparative-scale synthesis of pyrimidine or purine 2'-deoxy-beta-D-ribonucleosides from a heterocyclic base and 2'-deoxyuridine proceeds by biotransformation reaction in the presence of thymine-dependent E. coli mutant cells encapsulated in alginate gel. The products are isolated by octadecyl-silica or ion-exchange chromatography.
Holý
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A
Institute of Organic Chemistry and Biochemistry, Czechoslovak Academy of Sciences, Praha 6.
Votruba
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I
D001711:Biotransformation; D003853:Deoxyribonucleosides / Q000138:chemical synthesis* / Q000302:isolation & purification / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism*; D005782:Gels; D013941:Thymine / Q000378:metabolism
D003853:Deoxyribonucleosides; D005782:Gels; D013941:Thymine