[Protein damage after treatment with ozone of protoplasts and isolated E. coli membranes].
The features of ozone-induced damage of E. coli plasma membrane proteins are investigated. A conclusion is made that protein fluorescence quenching is connected with modification of amino acid residues in the vicinity of tryptophane residues. Such modification may be a consequence of reaction with either ozone itself or products of its interaction with membrane lipids and/or proteins. The suggestion of Goldstein and McDonagh that ozone has a predilection for more hydrophilical membrane domains is confirmed. The data obtained are in agreement with a supposition about the leading role of proteins in deleterious action of ozone on cells.
Matus
V K
VK
Kaler
G V
GV
Mel'nikova
A M
AM
Konev
S V
SV
D000596:Amino Acids / Q000032:analysis; D002462:Cell Membrane / Q000032:analysis / Q000187:drug effects; D004926:Escherichia coli / Q000032:analysis / Q000187:drug effects*; D007700:Kinetics; D008565:Membrane Proteins / Q000032:analysis*; D010084:Oxidation-Reduction; D010126:Ozone / Q000633:toxicity*; D011523:Protoplasts / Q000032:analysis / Q000187:drug effects; D013050:Spectrometry, Fluorescence
D000596:Amino Acids; D008565:Membrane Proteins; D010126:Ozone
Povrezhdenie belkov pri deĭstvii ozona na protoplasty i vydelennye membrany E. coli.