A simple method for controlled freezing of human donor corneae.
A simple method for freezing cultured human donor material with dimethyl sulfoxide (DMSO) was developed empirically in a commercially available microcomputer-controlled apparatus allowing reproducible freezing curves to be made. The optimum cooling rate was found to be 1.0 degrees C/min down to -40 degrees C and then 5.0 degrees C/min to -80 degrees C. Total cell loss rates were 36.9 +/- (SD) 19.8% of postmortem endothelial cell counts. An improved method for evaluating endothelial viability was applied, which enabled us to observe definite cell survival after a postculture period.
Böhnke
M
M
Draeger
J
J
Rethwisch
K
K
Stürmer
B
B
D003315:Cornea / Q000166:cytology; D016039:Corneal Transplantation; D003470:Culture Media; D004727:Endothelium / Q000166:cytology; D005615:Freezing / Q000379:methods*; D006801:Humans; D009924:Organ Culture Techniques
D003470:Culture Media