Expression of dengue virus structural proteins and nonstructural protein NS1 by a recombinant vaccinia virus.
A recombinant vaccinia virus containing cloned DNA sequences coding for the three structural proteins and nonstructural proteins NS1 and NS2a of dengue type 4 virus was constructed. Infection of CV-1 cells with this recombinant virus produced dengue virus structural proteins as well as the nonstructural protein NS1. These proteins were precipitated by specific antisera and exhibited the same molecular size and glycosylation patterns as authentic dengue virus proteins. Infection of cotton rats with the recombinant virus induced NS1 antibodies in 1 of 11 animals. However, an immune response to the PreM and E glycoproteins was not detected. A reduced level of gene expression was probably the reason for the limited serologic response to these dengue virus antigens.
Zhao
B T
BT
Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892.
Prince
G
G
Horswood
R
R
Eckels
K
K
Summers
P
P
Chanock
R
R
Lai
C J
CJ
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D000941:Antigens / Q000276:immunology*; D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology; D003411:Arvicolinae; D002460:Cell Line; D003001:Cloning, Molecular; D003716:Dengue Virus / Q000235:genetics* / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D005786:Gene Expression Regulation; D005814:Genes, Viral; D007114:Immunization; D007118:Immunoassay; D011863:Radioimmunoassay; D014614:Vaccines, Synthetic / Q000276:immunology*; D014616:Vaccinia virus / Q000235:genetics* / Q000276:immunology; D014764:Viral Proteins / Q000235:genetics* / Q000276:immunology; D015678:Viral Structural Proteins; D014765:Viral Vaccines / Q000276:immunology
D000914:Antibodies, Viral; D000941:Antigens; D000956:Antigens, Viral; D014614:Vaccines, Synthetic; D014764:Viral Proteins; D015678:Viral Structural Proteins; D014765:Viral Vaccines