Expression of FMC7 antigen and tartrate-resistant acid phosphatase isoenzyme in cases of B-lymphoproliferative diseases.
A panel of different B-cell malignancies representing various stages of B-cell differentiation were analyzed for the expression of an antigen labeled by the monoclonal antibody FMC7 and of tartrate-resistant acid phosphatase (TracP) activity. The FMC7 antigen and TracP were not found on early immature pre B-cell proliferations, appeared at early and intermediate B-cell stages, reached their peak of expression in terms of both incidence of positivity and staining intensity at the late B cell stage (as represented by hairy cell leukemia) and were lost at the B-cell/plasma cell transition. Although detected at similar stages of B-cell differentiation, FMC7 and TracP appear to be independently expressed and were not related to a particular Ig class. The simultaneous detection of FMC7 and TracP represents a distinguishing parameter for the identification of hairy cell leukemia.
Drexler
H G
HG
Menon
M
M
Gaedicke
G
G
Minowada
J
J
D000135:Acid Phosphatase / Q000276:immunology*; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000276:immunology*; D001402:B-Lymphocytes; D002478:Cells, Cultured; D005455:Fluorescent Antibody Technique; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000276:immunology*; D008232:Lymphoproliferative Disorders / Q000276:immunology* / Q000473:pathology
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D007527:Isoenzymes; D000135:Acid Phosphatase