The effect of neutrophil migration and prolonged neutrophil contact on epithelial permeability.
The effect of neutrophil migration and prolonged neutrophil contact on epithelial permeability was examined. Although neutrophil migration was not associated with a change in epithelial permeability, prolonged neutrophil-epithelial contact following migration resulted in an increase in epithelial permeability. These results were not altered by catalase, a specific neutrophil elastase inhibitor, methoxysuccinyl-Ala-Ala-Pro-Val-chloromethyl ketone or cyclohexamide. This suggests that neutrophil migration does not occur via an H2O2-induced reversible mechanism of junctional opening, which we describe herein.
Parsons
P E
PE
Department of Medicine, National Immunology and Respiratory Medicine, Denver, CO 80206.
Sugahara
K
K
Cott
G R
GR
Mason
R J
RJ
Henson
P M
PM
D000818:Animals; D002460:Cell Line; D002465:Cell Movement; D002470:Cell Survival; D004553:Electric Conductivity; D004847:Epithelial Cells; D004848:Epithelium / Q000502:physiology; D005455:Fluorescent Antibody Technique; D006861:Hydrogen Peroxide / Q000494:pharmacology; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D008564:Membrane Potentials; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D010196:Pancreatic Elastase / Q000378:metabolism; D010539:Permeability; D051381:Rats; D011919:Rats, Inbred Strains
D006861:Hydrogen Peroxide; D010196:Pancreatic Elastase