Binding of the aliphatic halides 1,2-dibromoethane and chloroform in the rodent vaginal epithelium.
Whole-body and light microscopic autoradiography were used to study the binding of 1,2-dibromo(14C)ethane (14C-DBE) and 14C-chloroform (14C-CF) in the mouse and rat vaginal epithelium in vitro and in vivo. In pregnant mice, mice pretreated with pregnant mare's serum gonadotropin (PMSG) or ovariectomized mice primed with medroxyprogesterone, a high level of bound 14C-DBE metabolites were present in the epithelium, while in ovariectomized oestradiol-primed mice or intact oestradiol-primed mice, the binding was low. Similar results were obtained with 14C-CF, although the level of binding generally was lower than that observed after 14C-DBE-exposure. No binding of 14C-DBE-metabolites was observed in the juvenile rat vaginal epithelium, whereas a high binding was present in the PMSG-primed adult rat vaginal epithelium. Collectively, these data show that 14C-DBE and 14C-CF are transformed in situ to metabolites that are irreversibly bound to the vaginal epithelium. The results also suggest that the activating enzyme is under endocrine control and has a low activity in the juvenile and oestradiol-primed adult animal.
Brittebo
E B
EB
Kowalski
B
B
Brandt
I
I
D000818:Animals; D001345:Autoradiography; D002725:Chloroform / Q000378:metabolism*; D004848:Epithelium / Q000378:metabolism; D004958:Estradiol / Q000494:pharmacology; D015946:Ethylene Dibromide / Q000378:metabolism*; D005260:Female; D006064:Gonadotropins, Equine / Q000494:pharmacology; D006842:Hydrocarbons, Brominated / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D010052:Ovariectomy; D011374:Progesterone / Q000494:pharmacology; D051381:Rats; D011916:Rats, Inbred F344; D014621:Vagina / Q000378:metabolism*
D006064:Gonadotropins, Equine; D006842:Hydrocarbons, Brominated; D015946:Ethylene Dibromide; D011374:Progesterone; D004958:Estradiol; D002725:Chloroform