Expression of cell-cycle-dependent genes in serum stimulated cells whose entry into S phase is blocked by cytochalasin D.
A low concentration (0.6 micrograms/ml) of cytochalasin D inhibits the initiation of DNA synthesis after serum stimulation of growth-arrested GC-7 cells. Since actin-containing structures are suggested to be involved in the transfer of the growth signal to nuclei and in the synthesis and transport of nascent RNA, the effect of cytochalasin D on the expression of cell-cycle-regulated genes after serum stimulation was studied by Northern blot analysis. Cytoplasmic accumulation of such mRNAs as or c-fos, c-myc, beta-actin an ornithine decarboxylase occurred in serum-stimulated cells regardless of the presence of cytochalasin D, whereas that of thymidine kinase and histone H3 was blocked by the drug.
Takasuka
T
T
Ishibashi
S
S
Ide
T
T
D000199:Actins / Q000235:genetics; D000818:Animals; D002453:Cell Cycle; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D015638:Cytochalasin D; D003572:Cytochalasins / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects*; D006657:Histones / Q000235:genetics; D007399:Interphase; D009955:Ornithine Decarboxylase / Q000235:genetics; D011518:Proto-Oncogene Proteins / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D013937:Thymidine Kinase / Q000235:genetics
D000199:Actins; D003572:Cytochalasins; D006657:Histones; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D015638:Cytochalasin D; D013937:Thymidine Kinase; D009955:Ornithine Decarboxylase