NAD+-dependent retinol dehydrogenase in liver microsomes.
A microsomal NAD+-dependent retinol dehydrogenase is being described with optimal activity at physiological pH. The enzyme was present in liver microsomes of rats and also in a strain of deermice which lacks the cytosolic retinol dehydrogenase. Unlike the latter enzyme, the microsomal retinol dehydrogenase was not inhibited by either ethanol or 4-methylpyrazole; its activity was insensitive to CO and not oxygen dependent, in contradistinction with that of the microsomal cytochrome P-450 and NADPH-dependent retinol oxidase. Chronic ethanol consumption resulted in an increased activity of the microsomal retinol dehydrogenase which may contribute to hepatic retinol depletion, especially in view of the insensitivity of the enzyme to ethanol inhibition.
Leo
M A
MA
Alcohol Research and Treatment Center, Bronx VA Medical Center, New York 10468.
Kim
C I
CI
Lieber
C S
CS
D000429:Alcohol Oxidoreductases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000818:Animals; D000072467:Cytochrome P450 Family 2; D003600:Cytosol / Q000201:enzymology; D000431:Ethanol / Q000494:pharmacology; D005260:Female; D007527:Isoenzymes / Q000037:antagonists & inhibitors / Q000378:metabolism; D007700:Kinetics; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology*; D009243:NAD / Q000378:metabolism*; D009249:NADP / Q000378:metabolism; D010542:Peromyscus; D051381:Rats; D011919:Rats, Inbred Strains; D012172:Retinaldehyde / Q000378:metabolism; D012727:Sex Characteristics; D014801:Vitamin A / Q000378:metabolism
D007527:Isoenzymes; D009243:NAD; D014801:Vitamin A; D000431:Ethanol; D009249:NADP; D000429:Alcohol Oxidoreductases; C022520:retinol dehydrogenase; C504568:Cyp2d1 protein, rat; D000072467:Cytochrome P450 Family 2; D012172:Retinaldehyde