title: Completion of the amino acid sequence of the alpha 1 chain of human basement membrane collagen (type IV) reveals 21 non-triplet interruptions located within the collagenous domain.
abstract: The cDNA and protein sequences of the N-terminal half of human basement membrane collagen (type IV) have been determined. Overlapping cDNA clones were constructed by repeated primer extension with synthetic oligonucleotides. They cover 2953 bp, beginning at the 5' end of the corresponding mRNA. At the protein level, the sequence of the cyanogen bromide peptide CB6 adjacent to the 7S domain has been additionally elucidated. The data presented here complete the protein sequence and nearly the entire cDNA sequence of the human alpha 1(IV) chain. The amino-terminal half of the alpha 1(IV) chain contains 8 cysteine residues involved in intramolecular and intermolecular cross-links. The entire triple-helical domain of alpha 1(IV) is interrupted by 21 non-triplet regions.
authors.affiliation: Max-Planck-Institut für Biochemie, Martinsried bei München, Federal Republic of Germany.
authors.forename: D
authors.initials: D
authors.lastname: Brazel
authors.forename: I
authors.initials: I
authors.lastname: Oberbäumer
authors.forename: H
authors.initials: H
authors.lastname: Dieringer
authors.forename: W
authors.initials: W
authors.lastname: Babel
authors.forename: R W
authors.initials: RW
authors.lastname: Glanville
authors.forename: R
authors.initials: R
authors.lastname: Deutzmann
authors.forename: K
authors.initials: K
authors.lastname: Kühn
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D001485:Basement Membrane / Q000032:analysis*; D003001:Cloning, Molecular; D003094:Collagen / Q000032:analysis* / Q000235:genetics; D003545:Cysteine / Q000032:analysis; D004247:DNA / Q000032:analysis; D005796:Genes; D006801:Humans; D006868:Hydrolysis; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000032:analysis; D010447:Peptide Hydrolases; D012333:RNA, Messenger / Q000032:analysis
chemical_list: D010446:Peptide Fragments; D012333:RNA, Messenger; D003094:Collagen; D004247:DNA; D010447:Peptide Hydrolases; D003545:Cysteine