[Use of immunological adsorption in a heterologous immunoenzyme analytical system for detecting specific antibodies to serogroup B and D1 Salmonella lipopolysaccharides in healthy donors and in persons with a history of salmonellosis].
The sera obtained from healthy donors and from subjects with a history of Salmonella infections (B, C1, D1 and E1) of medium severity were studied. In the initial testing in the heterologous enzyme immunoassay the levels of antibodies to LPS, groups B and D1, were somewhat higher in the subjects with a history of the infections than in healthy donors. After a single adsorption with homologous LPS the levelling of differences between the two groups was observed. After two adsorptions with heterologous LPS the level of the reaction in both the groups increased, and the differences between these groups augmented. The adsorption of sera with heterologous LPS increased the diagnostic sensitivity of the reaction only to 14-23% in the determination of the serogroup of the causative agent. An increase in the antibody level after adsorption with heterologous LPS was supposedly due to the additional detection of group-specific antibodies with low avidity, which appeared as the result of the elimination of cross-reacting highly avid antibodies, as well as non-immunoglobulin molecules complementary to LPS.
Pokrovskiĭ
V I
VI
Abalakin
V A
VA
D000327:Adsorption; D000328:Adult; D000907:Antibodies, Bacterial / Q000032:analysis*; D000915:Antibody Affinity; D000918:Antibody Specificity; D001782:Blood Donors; D003429:Cross Reactions; D005069:Evaluation Studies as Topic; D006801:Humans; D007124:Immunoenzyme Techniques / Q000295:instrumentation; D008070:Lipopolysaccharides / Q000276:immunology*; D012480:Salmonella Infections / Q000175:diagnosis*; D012477:Salmonella enteritidis / Q000145:classification / Q000276:immunology*; D012486:Salmonella typhimurium / Q000145:classification / Q000276:immunology*; D012703:Serotyping
D000907:Antibodies, Bacterial; D008070:Lipopolysaccharides
Primenenie immunologicheskoĭ adsorbtsii v sisteme geterogennogo immunofermentnogo analiza pri vyiavlenii spetsificheskikh antitel k sal'monelleznym lipopolisakharidam serogrupp B i D1 u zdorovykh donorov i perebolevshikh sal'monellezami liudeĭ.