title: Synthesis and localization of pathogenesis-related proteins in tobacco.
abstract: The PR1 family of pathogenesis-related proteins from tobacco (Nicotiana tabacum L.) leaves is induced by a variety of pathogenic and chemical agents and is associated with resistance to tobacco mosaic virus. The majority of the PR1 proteins did not copurify with mesophyll protoplasts (the major cell type of the leaf) isolated from tobacco mosaic virus-infected N. tabacum cv. Xanthi-nc leaves. However, these isolated protoplasts were capable of synthesizing and selectively secreting the PR1 proteins. Using monoclonal antibodies for immunofluorescence microscopy, we localized these proteins to the extracellular spaces predominantly in regions adjacent to viral lesions as well as in xylem elements of infected leaves.
authors.forename: J P
authors.initials: JP
authors.lastname: Carr
authors.forename: D C
authors.initials: DC
authors.lastname: Dixon
authors.forename: B J
authors.initials: BJ
authors.lastname: Nikolau
authors.forename: K V
authors.initials: KV
authors.lastname: Voelkerding
authors.forename: D F
authors.initials: DF
authors.lastname: Klessig
mesh_terms: D000911:Antibodies, Monoclonal; D005455:Fluorescent Antibody Technique; D010944:Plants / Q000382:microbiology*; D010947:Plants, Toxic; D014026:Nicotiana / Q000382:microbiology; D014027:Tobacco Mosaic Virus / Q000235:genetics*; D014764:Viral Proteins / Q000096:biosynthesis* / Q000302:isolation & purification
chemical_list: D000911:Antibodies, Monoclonal; D014764:Viral Proteins