HLA-DR antigen expression on T cells from cerebrospinal fluid in multiple sclerosis and aseptic meningo-encephalitis.
HLA-DR expression on T lymphocytes in cerebrospinal fluid (CSF) from patients with multiple sclerosis (MS) and acute aseptic meningo-encephalitis (AM), and from blood only from healthy controls was examined by a new double-immunofluorescence labelling assay using species-specific second layers on prefixed cell samples. Thirteen of 16 patients with AM (81%) had an elevated percentage of DR positive T cells in CSF against only two of 20 patients with MS (10%). Our data indicate that AM, an acute infection of the central nervous system (CNS), is accompanied by accumulation in CSF of activated, DR positive T cells as a reflection of actively involved cellular immunity within the CNS, while this accumulation of DR positive T cells is not seen in MS, a chronic inflammatory CNS disease, despite some of the patients being examined during clinical exacerbations.
Fredrikson
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S
Department of Neurology, Karolinska Institutet, Huddinge University Hospital, Stockholm, Sweden.
Karlsson-Parra
A
A
Olsson
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T
Link
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H
D000208:Acute Disease; D000328:Adult; D005260:Female; D005455:Fluorescent Antibody Technique; D006681:HLA-D Antigens / Q000134:cerebrospinal fluid*; D006684:HLA-DR Antigens / Q000134:cerebrospinal fluid*; D006801:Humans; D008297:Male; D008590:Meningoencephalitis / Q000134:cerebrospinal fluid / Q000276:immunology*; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000134:cerebrospinal fluid / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
D006681:HLA-D Antigens; D006684:HLA-DR Antigens