Different levels of DNA methylation in yeast and mycelial forms of Candida albicans.
Isotope dilution gas chromatography-mass spectrometry analysis of genomic DNAs isolated from three Candida albicans isolates showed significant differences in the amounts of 5-methyldeoxycytidine (m5Cyt) in DNA from yeast-form and from mycelial-form cells; the moles percent m5Cyt were 0.11, 0.11, and 0.097 for yeast-form DNA and 0.045, 0.053, and 0.047 for mycelial-form DNA for the three isolates Sh8, 9938, and B311, respectively. The lower m5Cyt values for mycelial-form cells suggest that those cells may exhibit significantly greater gene activity than yeast-form cells.
Russell
P J
PJ
Welsch
J A
JA
Rachlin
E M
EM
McCloskey
J A
JA
D002176:Candida albicans / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D003470:Culture Media; D003596:Cytosine / Q000378:metabolism; D004271:DNA, Fungal / Q000032:analysis* / Q000378:metabolism; D003841:Deoxycytidine / Q000031:analogs & derivatives* / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry; D005800:Genes, Fungal; D006801:Humans; D008745:Methylation
D003470:Culture Media; D004271:DNA, Fungal; D003841:Deoxycytidine; D003596:Cytosine; C016569:5-methyldeoxycytidine