Enhanced preservation of endosecretory granules in PP-cells of the canine pancreas.
Standard fixation techniques commonly used for light and electron microscopic studies have resulted in reported differences in the ultrastructural appearance of endosecretory granules of the pancreatic polypeptide (PP) cell. To clarify these differences, canine pancreatic tissues of intact and cultured pseudoislets were studied using a variety of ingredients, additives and fixatives in an effort to better preserve the endosecretory granules of PP cells. Results show that preservation of PP granules is enhanced by addition in zinc chloride (0.5%) to a glutaraldehyde-paraformaldehyde fixative in 0.1 M cacodylate buffer, followed by osmium tetroxide fixation. This fixative is recommended for all light and electron microscopic studies of the pancreatic polypeptide cell.
Greider
M H
MH
Department of Pathology, Washington University School of Medicine, St. Louis, MO 63110.
Scharp
D W
DW
Gingerich
R L
RL
D000818:Animals; D003584:Cytological Techniques; D003594:Cytoplasmic Granules / Q000648:ultrastructure*; D004285:Dogs; D005404:Fixatives; D007515:Islets of Langerhans / Q000166:cytology* / Q000648:ultrastructure; D008854:Microscopy, Electron; D010191:Pancreatic Polypeptide
D005404:Fixatives; D010191:Pancreatic Polypeptide