title: Molecular cloning of the cDNA for tissue factor, the cellular receptor for the initiation of the coagulation protease cascade.
abstract: We have isolated cDNA clones encoding the complete sequence of the heavy chain of tissue factor (TF), the high-affinity receptor responsible for cellular initiation of the coagulation protease cascade. An 885 bp open reading frame encodes a 295 amino acid polypeptide including a leader sequence with alternative cleavage sites. A single 2.3 kb mRNA is identified, and Southern blotting is consistent with a single gene. The coding sequence defines a protein with features characteristic of an integral membrane protein. This receptor appears novel, lacking significant homology with other proteins; however, TF contains the uncommon tryptophan-lysine-serine (WKS) sequence repeated three times, a sequence we find in some serine protease-binding proteins and suggest may represent a functional sequence motif.
authors.forename: J H
authors.initials: JH
authors.lastname: Morrissey
authors.forename: H
authors.initials: H
authors.lastname: Fakhrai
authors.forename: T S
authors.initials: TS
authors.lastname: Edgington
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D001777:Blood Coagulation; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D010450:Endopeptidases / Q000378:metabolism; D006801:Humans; D012697:Serine Endopeptidases; D013925:Thromboplastin / Q000235:genetics*
chemical_list: D004247:DNA; D013925:Thromboplastin; D010450:Endopeptidases; D012697:Serine Endopeptidases