Immunohistochemical studies of the distribution of a basolateral-membrane protein in intestinal epithelial cells (GZ1-Ag) in rats using monoclonal antibodies.
The monoclonal antibody (mAb), GZ1, is specific for a 42-kilodalton (kD) protein (designated GZ1-Ag) present among the plasma membrane (PM) proteins of the absorptive cells of rat intestine. This protein only occurs in the basolateral PM and is absent from the microvillus membrane. GZ2 and GZ20 are two other mAbs that are also directed against GZ1-Ag but which specify other antigenic determinants of this protein than mAb GZ1. Used together, these three mAbs allow better characterization of GZ1-Ag and more precise investigation of its distribution and localization in various rat cells. We performed immunohistochemical labelling for GZ1-Ag at both the light- and electron-microscope levels and found that GZ1-Ag is extensively distributed in rat epithelial tissues. However, the amount of this protein present in epithelial tissue shows considerable variation. GZ1-Ag is not present in the secretory cells of terminal portions of most excretory glands or in cells of the endocrine glands and liver. The cells of kidney tubules, except for collecting tubules, also lack GZ1-Ag. Only small amounts of GZ1-Ag are present in the cells of the stratified squamous epithelium and transitional epithelium, the exception being superficial cells. High concentrations of GZ1-Ag occur in the excretory duct systems of glands and in the various kinds of epithelium present in the male and female genital tract. Our results also indicated that the GZ1-Ag in all of these cells has a very similar structure.(ABSTRACT TRUNCATED AT 250 WORDS)
Schiechl
H
H
Institut für Histologie und Embryologie, Universität Graz, Austria.
Dohr
G
G
D000818:Animals; D000911:Antibodies, Monoclonal; D005455:Fluorescent Antibody Technique; D006046:Gold; D007413:Intestinal Mucosa / Q000032:analysis*; D008565:Membrane Proteins / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains
D000911:Antibodies, Monoclonal; D008565:Membrane Proteins; D006046:Gold