title: Human growth hormone produced with recombinant DNA technology: development and production.
abstract: The molecular basis of recombinant DNA technology is described, and the principles of genetically engineered proteins developed. The production of hGH by such methods utilizes a strain of Escherichia coli as host and a vector plasmid containing the appropriate information. Fermentation and purification of the hGH produced gives a preparation of high purity, containing only 1-2 ppm of E. coli polypeptide (ECP). This somatrem (Somatonorm) is identical to pituitary hGH except for an additional methionine residue at the N-terminal. Monoclonal antibodies fail to distinguish between pituitary hGH and somatrem. Preclinical studies of a variety of pharmacological and toxicological parameters indicate that the two hGH preparations have identical biological effects; no toxicological or mutagenic effects of somatrem have been detected.
authors.forename: H
authors.initials: H
authors.lastname: Flodh
mesh_terms: D003001:Cloning, Molecular; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000235:genetics; D013006:Growth Hormone / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification; D006728:Hormones / Q000302:isolation & purification; D019382:Human Growth Hormone; D006801:Humans; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D004274:DNA, Recombinant; D006728:Hormones; D011994:Recombinant Proteins; D019382:Human Growth Hormone; D013006:Growth Hormone