[Determination of ovalbumin-specific IgG antibodies in mouse sera by immunoenzyme analysis using horseradish peroxidase-conjugated staphylococcal protein A].
The article deals with the possibility of using staphylococcal protein A conjugated with horse-radish peroxidase for the detection of specific IgG-antibodies to ovalbumin in mice by the indirect and competitive EIA techniques. Studies on specifying the parameters of the EIA system for the detection of specific IgG-antibodies are in progress.
Popov
A N
AN
Gushchin
I S
IS
Bulk
V F
VF
Perel'man
E V
EV
D000818:Animals; D000918:Antibody Specificity; D006967:Hypersensitivity / Q000276:immunology; D007114:Immunization; D007124:Immunoenzyme Techniques / Q000295:instrumentation; D007074:Immunoglobulin G / Q000032:analysis*; D051379:Mice; D008808:Mice, Inbred CBA; D010047:Ovalbumin / Q000276:immunology*; D011137:Polystyrenes; D013205:Staphylococcal Protein A
D007074:Immunoglobulin G; D011137:Polystyrenes; D013205:Staphylococcal Protein A; D010047:Ovalbumin
Opredelenie spetsificheskikh IgG-antitel k oval'buminu v syvorotkakh mysheĭ metodom immunofermentnogo analiza s ispol'zovaniem stafilokokkovogo proteina A, kon''iugirovannogo s peroksidazoĭ khrena.