The Golgi apparatus-complex of neurons and astrocytes studied with an anti-organelle antibody.
An antiserum reacting with a 135-kDa antigen of rat liver Golgi apparatus-complex was used to stain, by light microscopic and ultrastructural immunocytochemistry, sections of rat cerebellum and by immunoblot homogenates of whole brain, isolated neurons and a fraction of enriched neuronal Golgi apparatus. In sections of rat cerebellum fixed with periodate-lysine-paraformaldehyde and immunostained with the direct peroxidase or peroxidase-antiperoxidase methods, the Golgi apparatus-complex in perikarya of neurons and glia was stained. Occasionally, nuclear envelopes and cisternae of the rough endoplasmic reticulum of neurons and glia were stained. Immunostain was not observed in peripheral dendrites, axons and presynaptic terminals. In striking contrast, peripheral smooth cisternae of astrocytic perikarya and processes were stained. Immunoblots of whole-brain membrane fractions, homogenates of isolated neurons and an enriched neuronal fraction of the Golgi apparatus-complex showed a principal single band of 64-kDa apparent mol. wt. We have concluded that the putative 64-kDa antigen(s) is distributed in cisternae of the Golgi apparatus-complex and occasionally in the nuclear envelope and rough reticulum, within the perikarya of neurons and glia. A second important distribution of the 64-kDa antigen(s), involving peripheral cisternae in perikarya and processes of astrocytes, is consistent with the hypothesis that the Golgi apparatus-complex of these cells extends to the periphery of these cells. The functional implications of the peripheral localization of the 64-kDa antigen(s) in astrocytes are discussed.
Stieber
A
A
Gonatas
J O
JO
Gonatas
N K
NK
Louvard
D
D
D000818:Animals; D001253:Astrocytes / Q000032:analysis* / Q000648:ultrastructure; D001923:Brain Chemistry; D002531:Cerebellum / Q000032:analysis* / Q000648:ultrastructure; D004591:Electrophoresis, Polyacrylamide Gel; D006056:Golgi Apparatus / Q000032:analysis* / Q000276:immunology; D007106:Immune Sera; D007158:Immunologic Techniques; D008854:Microscopy, Electron; D008970:Molecular Weight; D009474:Neurons / Q000032:analysis* / Q000648:ultrastructure; D051381:Rats; D011917:Rats, Inbred Lew; D013347:Subcellular Fractions / Q000032:analysis
D007106:Immune Sera