Carbohydrate analysis of chicken heart glycolipids.
Neutral and acidic glycolipids were extracted from chicken hearts. The neutral and acidic compounds were separated by preparative thin-layer chromatography into eight and two fractions, respectively. Total hydrolysis by mineral acid, permethylation analysis, and sequential cleavage with exoglycosidases showed the presence of glycolipids that belong to the globo- and gala-oligosaccharide series, i.e., the monohexosylceramides Glc-Cer and Gal-Cer, the dihexosylceramides Gal beta 1-4Glc-Cer and Gal alpha 1-4Gal-Cer, the tetrahexosylceramides GalNAc beta 1-3Gal alpha 1-4Gal beta 1-4Glc-Cer and GalNAc alpha 1-3GalNAc beta 1-3Gal alpha 1-4Gal-Cer (III3GalNAc alpha-Ga3Cer) and four subfractions of the Forssman glycolipid GalNAc alpha 1-3GalNAc beta 1-3Gal alpha 1-4Gal beta 1-4Glc-Cer. With the notable exception of III3GalNAc alpha 1-Ga3Cer, all glycolipids with terminal GalNAc alpha 1-3GalNAc1 reacted on thin-layer chromatograms with a monoclonal anti-Forssman antibody. The major components of the acidic fraction glycolipids were characterized as the lactose-based gangliosides Glac1 (GM3) and Glac2 (GD3).
Jennemann
R
R
Physiologisch-Chemisches Institut, Philipps-Universität Marburg, Federal Republic of Germany.
Felding-Habermann
B
B
Geyer
R
R
Stirm
S
S
Wiegandt
H
H
D000818:Animals; D000911:Antibodies, Monoclonal; D002236:Carbohydrate Conformation; D002241:Carbohydrates / Q000032:analysis*; D002645:Chickens; D002855:Chromatography, Thin Layer; D005577:Forssman Antigen / Q000276:immunology; D005690:Galactose / Q000032:analysis; D005732:Gangliosides / Q000032:analysis; D005947:Glucose / Q000032:analysis; D006017:Glycolipids / Q000032:analysis*; D006026:Glycoside Hydrolases / Q000378:metabolism; D006028:Glycosphingolipids / Q000032:analysis; D007163:Immunosorbent Techniques; D009206:Myocardium / Q000032:analysis*
D000911:Antibodies, Monoclonal; D002241:Carbohydrates; D005732:Gangliosides; D006017:Glycolipids; D006028:Glycosphingolipids; D005577:Forssman Antigen; D006026:Glycoside Hydrolases; D005947:Glucose; D005690:Galactose