Fluorescence and chemiluminescence enzyme immunoassays of 17 alpha-hydroxyprogesterone in dried blood spotted on filter paper.
Enzyme immunoassays for 17 alpha-hydroxyprogesterone (17-OHP) were developed. Horseradish peroxidase (HRP), glucose oxidase (GOD), invertase (INV) and glucose-6-phosphate dehydrogenase (G6PDH) were used as label enzymes. Double antibody coated beads or tubes were used for separating the bound and free fractions. Antisera used were prepared by using 4-carboxyethylthio-17-OHP and 3-carboxymethyl oxime-17-OHP-bovine serum albumin as immunogens. The bridge heterologous system was more sensitive than other site heterologous and homologous systems. The minimum amounts of 17-OHP detected were 0.25 and 1.0 pg/tube for fluorescence EIAs using HRP and GOD, and 0.1, 10 and 0.1 pg/tube for chemiluminescence EIAs using GOD, INV and G6PDH, respectively. The reproducibility and correlation with RIA were also studied. The present study demonstrates the feasibility of a neonatal screening for congenital adrenal hyperplasia.
Tsuji
A
A
School of Pharmaceutical Sciences, Showa University, Tokyo, Japan.
Maeda
M
M
Arakawa
H
H
Shimizu
S
S
Ikegami
T
T
Sudo
Y
Y
Hosoda
H
H
Nambara
T
T
D019326:17-alpha-Hydroxyprogesterone; D005455:Fluorescent Antibody Technique; D006801:Humans; D006908:Hydroxyprogesterones / Q000097:blood*; D007124:Immunoenzyme Techniques; D008163:Luminescent Measurements
D006908:Hydroxyprogesterones; D019326:17-alpha-Hydroxyprogesterone