Identification of tissue histiocytes on paraffin sections by a new monoclonal antibody.
A mouse monoclonal antibody (MAC 387) with specificity for monocytes and tissue histiocytes was produced by immunization of a BALB/c mouse with peripheral blood monocyte components derived by affinity chromatography of detergent-solubilized monocyte material on Sepharose 4B coupled to rabbit anti-monocyte antibodies. MAC 387 strongly stained the cytoplasm of cells of the monocyte/macrophage series on paraffin sections after controlled trypsinization of sections. The antibody showed broad reactivity for a variety of tissue histiocytes, including infiltrating and reactive histiocytes, alveolar macrophages, Kupffer cells, follicle-center macrophages, splenic red pulp macrophages, tumor-infiltrating macrophages, sinus histiocytes, epithelioid giant cells (variably), and cases of histiocytosis X and dermatopathic lymphadenopathy. Molecular weight data obtained by Western blotting, immunoprecipitation, and immunoaffinity-purification revealed that the antigen was present in different forms in the monocyte and granulocyte. In the granulocyte, free alpha (Mr 12 KD) and beta (Mr 14 KD) chains expressing the MAC 387 epitope were found together with associations of one alpha and one beta chain linked by disulfide bonds to yield a heterodimer of Mr 26 KD. In the monocyte, free alpha and beta chains are not found, but instead the heterodimer and associations of two (Mr 56 KD) and four (Mr 112 KD) heterodimers are disulfide-linked together. This new monoclonal reagent should have particular value for identification of tissue histiocytes in routine paraffin sections and particularly for demonstration of histiocytes in malignant lymphomas.
Flavell
D J
DJ
University Department of Pathology, Southampton General Hospital, United Kingdom.
Jones
D B
DB
Wright
D H
DH
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis* / Q000276:immunology*; D000954:Antigens, Surface / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D006098:Granulocytes / Q000276:immunology; D006644:Histiocytes / Q000166:cytology* / Q000276:immunology; D006646:Histiocytosis, Langerhans-Cell / Q000473:pathology; D006651:Histocytochemistry; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D007118:Immunoassay; D007124:Immunoenzyme Techniques; D007163:Immunosorbent Techniques; D008206:Lymphatic Diseases / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D009000:Monocytes / Q000276:immunology
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D007106:Immune Sera