Regulation of c-myc transcription and protein expression during activation of normal human B cells.
The close link between an actively expressed c-myc gene locus and cellular proliferation has been established in a variety of cell types. By using normal human peripheral blood B cells as a model for in vivo quiescence, we have assessed the expression pattern of the c-myc gene in terms of transcriptional activation and concurrent production of c-myc protein, following induction by antibodies directed against antigens on the cell membrane. Both the 1F5 monoclonal antibody, which reacts with the pan B cell antigen CD20, and the anti-mu could promote transcriptional activation of the c-myc gene roughly corresponding to the increased levels of cytoplasmic c-myc mRNAs. In addition, more than 80% of the B cells could be induced to express c-myc protein by either stimulus. Since treatment only with polyclonal anti-mu renders the B cells competent to proliferate in the presence of BCGF, c-myc protein expression is not per se sufficient for cell cycle progression into S phase.
Smeland
E B
EB
Department of Pathology, Norwegian Radium Hospital, Montebello, Oslo.
Beiske
K
K
Ek
B
B
Watt
R
R
Pfeifer-Ohlsson
S
S
Blomhoff
H K
HK
Godal
T
T
Ohlsson
R
R
D001402:B-Lymphocytes / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D008213:Lymphocyte Activation; D014176:Protein Biosynthesis; D011518:Proto-Oncogene Proteins / Q000032:analysis / Q000235:genetics; D011519:Proto-Oncogenes; D014158:Transcription, Genetic
D011518:Proto-Oncogene Proteins