Demonstration of histaminergic neurons in horizontal cells of guinea pig retina.
The existence of L-histidine decarboxylase (HDC, EC 4.1.1.22)-like immunoreactive (HDC-I) cells in guinea pig retina was demonstrated using antiserum raised against HDC purified from fetal rat liver. The anti-HDC antiserum partially cross-reacted guinea pig L-DOPA decarboxylase (DDC, EC 4.1.1.28), so the histaminergic neurons were carefully identified. Comparison of HDC-I and DDC-like immunoreactive (DDC-I) cell types in adjacent sections revealed that HDC-I structures were found in some horizontal cells and amacrine cells, and double-staining procedures with anti-HDC antiserum and monoclonal anti-DDC antibody showed that HDC-I horizontal cells had no DDC-I structures, but all the HDC-I amacrine cells had DDC-I structures. From the results, some horizontal cells (with HDC-like immunoreactivities but without DDC-like immunoreactivities) were concluded to be histaminergic.
Ando-Yamamoto
M
M
Kiyama
H
H
Hayashi
H
H
Fukui
H
H
Tohyama
M
M
Watanabe
T
T
Wada
H
H
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D002262:Carboxy-Lyases / Q000032:analysis*; D004296:Dopa Decarboxylase / Q000032:analysis; D005455:Fluorescent Antibody Technique; D006168:Guinea Pigs; D006632:Histamine / Q000032:analysis*; D006640:Histidine Decarboxylase / Q000032:analysis*; D006651:Histocytochemistry; D008297:Male; D009474:Neurons / Q000032:analysis; D012160:Retina / Q000032:analysis* / Q000166:cytology / Q000201:enzymology
D000911:Antibodies, Monoclonal; D006632:Histamine; D002262:Carboxy-Lyases; D004296:Dopa Decarboxylase; D006640:Histidine Decarboxylase