Enumeration of Trypanosoma cruzi-specific antibody-secreting cells by solid-phase immunoenzymatic technique.
A specific and sensitive enzyme-linked immunoassay is applied for enumeration of cells secreting specific antibodies to Trypanosoma cruzi antigens. Spleen cells from immunized mice are incubated in antigen-coated plastic tissue culture plates. Individual antibody-producing cells secrete antibody which binds to the antigen at close vicinity of the cells. The areas of bound antibodies are demonstrated by an enzyme-linked antibody assay as dark round spots, which can be easily enumerated. This assay allows the enumeration of specific antibody-secreting cells to parasite antigens, overcoming the failures to develop conventional plaque-forming cells assays to complex antigens.
Caulada
Z
Z
Departamento de Imunologia, Universidade de São Paulo, Brasil.
Abrahamsohn
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IA
D000818:Animals; D000913:Antibodies, Protozoan / Q000096:biosynthesis*; D000921:Antibody-Producing Cells / Q000276:immunology*; D000953:Antigens, Protozoan / Q000276:immunology; D002478:Cells, Cultured; D004306:Dose-Response Relationship, Immunologic; D007114:Immunization; D007124:Immunoenzyme Techniques / Q000592:standards*; D051379:Mice; D008815:Mice, Inbred Strains; D013154:Spleen / Q000166:cytology; D014349:Trypanosoma cruzi / Q000276:immunology*
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan