[Behavior of the large fragment of DNA polymerase I (the Klenow fragment) during fractionation of a cell-free extract of E. coli MRE-600].
Distribution of the DNA polymerase I large fragment (Klenow fragment) was studied during fractionation of the E. coli MRE-600 cell-free extract with polyethylenimine. On the basis of the results obtained a simple procedure is proposed that enables the Klenow fragment to be obtained as a coproduct of DNA polymerase I, RNA polymerase, polynucleotide phosphorylase, nucleotide kinases with acetokinase and nucleoside deoxy-ribosyltransferase in the framework of a combined technological scheme.
Khomov
V V
VV
Zagrebel'nyĭ
S N
SN
Legostaeva
G A
GA
Oreshkova
S F
SF
D005591:Chemical Fractionation; D004256:DNA Polymerase I / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000032:analysis / Q000201:enzymology* / Q000235:genetics; D008970:Molecular Weight; D009876:Operon
D004256:DNA Polymerase I
Povedenie bol'shogo fragmenta DNK-polimerazy I (fragmenta Klenova) pri fraktsionirovanii beskletochnogo ékstrakta E. coli MRE-600.