title: Analysis of tissue lymphocytes by double fluorescent staining--gastric cancer tissue and regional lymph nodes.
abstract: An immunohistochemical study was performed on human lymphocytes in the tissue of gastric cancer, and also in the regional lymph nodes, by double fluorescent staining, using monoclonal antibodies. Leu3a+8+ cells (inducer T cells) which consist about 30 per cent of Leu 3a+ cells were seen in the tissue surrounding the gastric cancer. The other 70 per cent of Leu 3a+ cells were Leu3a+8- cells (helper T cells). In the lymph nodes they were noted in T cell areas in almost the same proportions, while in germinal centers, only Leu3a+8- cells were found. On the other hand, OKT8+Leu15- cells (cytotoxic T cells) were noted in a large number, while OKT8+Leu15+ cells (suppressor T cells) were few. Further, an increase of OKT8+Leu15- cells was seen around gastric cancer or metastatic cancer in lymph nodes. These immunohistochemical findings suggest that cytotoxic T cells are the main component in the tissue of gastric cancers and the regional lymph nodes. Increases in inducer T cells and helper T cells are probably required to induce cytotoxic T cells around the cancer tissue.
authors.forename: I
authors.initials: I
authors.lastname: Takeda
authors.forename: T
authors.initials: T
authors.lastname: Horimi
authors.forename: T
authors.initials: T
authors.lastname: Okabayashi
authors.forename: S
authors.initials: S
authors.lastname: Nagae
authors.forename: S
authors.initials: S
authors.lastname: Chono
authors.forename: N
authors.initials: N
authors.lastname: Tokuda
authors.forename: K
authors.initials: K
authors.lastname: Orita
mesh_terms: D000911:Antibodies, Monoclonal; D005455:Fluorescent Antibody Technique; D006801:Humans; D008198:Lymph Nodes / Q000473:pathology*; D013270:Stomach / Q000473:pathology; D013274:Stomach Neoplasms / Q000473:pathology*; D013601:T-Lymphocytes / Q000145:classification / Q000473:pathology*
chemical_list: D000911:Antibodies, Monoclonal