Ca2+/calmodulin-dependent protein kinase in Saccharomyces cerevisiae.
Ca2+-dependent chromatography of soluble cytosolic proteins on calmodulin-Sepharose gave a fraction that exhibited Ca2+- and calmodulin-dependent phosphorylation of several polypeptides, including 60, 56 and 45 kDa species. At 0.2 microM beef calmodulin the phosphorylation was optimal at 3 microM free Ca2+, and at 80 microM free Ca2+ it was half-maximal at about 0.1 microM beef calmodulin. It is concluded that the fraction contains calmodulin-dependent protein kinase(s) which is (are) autophosphorylated or associated with substrates.
Londesborough
J
J
Nuutinen
M
M
D002845:Chromatography / Q000379:methods; D010455:Peptides / Q000302:isolation & purification; D010766:Phosphorylation; D011494:Protein Kinases / Q000302:isolation & purification*; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D017871:Calcium-Calmodulin-Dependent Protein Kinases
D010455:Peptides; D011494:Protein Kinases; D017871:Calcium-Calmodulin-Dependent Protein Kinases