Immuno-electron-microscopic application of antiserum against elastin.
The antiserum against insoluble elastin from human aorta was applied to immuno-electron microscopy. In the preembedding method, only the outer surface of the amorphous component (elastin) of elastic fibers showed a positive reaction. A major problem encountered with the preembedding method is associated with the penetration of either the primary antiserum or the secondary antibody into the tissue and, in particular, into elastin. On the contrary, a positive reaction was observed inner zones of elastin in the postembedding method. While the postembedding method employed here has limitations with nonspecific binding to the embedding medium, the postembedding method offers a decided advantage over the preembedding method.
Akita
M
M
Department of Anatomy, Saitama Medical School, Japan.
Fujita
K
K
Murata
E
E
Kaneko
K
K
Merker
H J
HJ
D000818:Animals; D000831:Animals, Newborn; D001011:Aorta / Q000032:analysis / Q000648:ultrastructure*; D004549:Elastin / Q000032:analysis* / Q000276:immunology; D006801:Humans; D007106:Immune Sera; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D051379:Mice; D008854:Microscopy, Electron
D007106:Immune Sera; D004549:Elastin