title: An apparatus for continuous analysis of protease-catalyzed acyl transfer reactions.
abstract: An apparatus that allows continuous analysis of protease-catalyzed acyl transfer reactions is described. Hydrolysis reaction is assayed using automatic titration. A continuous determination of amino group concentration by reaction with o-phthalaldehyde gives the rate of peptide bond formation. The apparatus allows the determination of the partition constant for the nucleophile at various nucleophile concentrations from one run.
authors.affiliation: Sekton Biowissenschaften der Karl-Marx-Universität Leipzig, German Democratic Republic.
authors.forename: V
authors.initials: V
authors.lastname: Schellenberger
authors.forename: U
authors.initials: U
authors.lastname: Schellenberger
authors.forename: Y V
authors.initials: YV
authors.lastname: Mitin
authors.forename: H D
authors.initials: HD
authors.lastname: Jakubke
mesh_terms: D000217:Acyltransferases; D001322:Autoanalysis / Q000295:instrumentation / Q000379:methods; D002918:Chymotrypsin / Q000378:metabolism; D006868:Hydrolysis; D007202:Indicators and Reagents; D007700:Kinetics; D010447:Peptide Hydrolases / Q000378:metabolism*; D009764:o-Phthalaldehyde
chemical_list: D007202:Indicators and Reagents; D009764:o-Phthalaldehyde; D000217:Acyltransferases; D010447:Peptide Hydrolases; D002918:Chymotrypsin