Immunocytochemical localization of acetylcholine receptors in locust brain using auto-anti-idiotypic acetylcholine antibodies.
Auto-anti-idiotypic antibodies have been detected in antisera of rabbits immunized with an acetylcholine (ACh) conjugate. These antibodies were found to bind to ACh receptor (ACh-R) purified from different species membranes. They competed with the ACh-R antagonist alpha-bungarotoxin and some agonists such as ACh conjugate and ACh itself. They did not recognize acetylcholinesterase. Their characterization 'in vitro' suggested their employment as an immunohistological marker for ACh-R. In the locust brain, specific immunoreactivity was found in neuropils of the protocerebrum, the optic lobes, the deutocerebrum and the tritocerebrum.
Vieillemaringe
J
J
Laboratoire de Neuroendocrinologie, UA C.N.R.S. 1138, Université de Bordeaux I, Talence, France.
Souan
M L
ML
Grandier-Vazeilles
X
X
Geffard
M
M
D000109:Acetylcholine / Q000276:immunology*; D000818:Animals; D001323:Autoantibodies / Q000302:isolation & purification / Q000378:metabolism; D001667:Binding, Competitive; D005260:Female; D005455:Fluorescent Antibody Technique; D005724:Ganglia / Q000032:analysis / Q000166:cytology; D006110:Grasshoppers / Q000032:analysis*; D006651:Histocytochemistry; D006801:Humans; D009132:Muscles / Q000378:metabolism; D009474:Neurons / Q000032:analysis*; D011817:Rabbits; D011950:Receptors, Cholinergic / Q000032:analysis*; D013045:Species Specificity; D013347:Subcellular Fractions / Q000378:metabolism
D001323:Autoantibodies; D011950:Receptors, Cholinergic; D000109:Acetylcholine