Quantitative determination of the lectin binding capacity of small intestinal brush-border membrane. An enzyme linked lectin sorbent assay (ELLSA).
A test to determine quantitatively the lectin binding sites in brush-border membranes has been developed. Highly purified bovine small intestinal brush-border membranes were prepared, and subsequently coated directly to the bottom of a microtiter plate. Soybean agglutinin conjugated with peroxidase was coupled to its binding sites in the brush-border membranes and the peroxidase activity was determined in a spectrophotometer. The number of soybean agglutinin binding sites in the brush-border membranes has been established by means of iterized computer fit analysis of the data, indicating values for maximal binding of 7.10(-7) M soybean agglutinin per mg of brush-border membrane protein and a dissociation constant of 1.5.10(-5) M.
Hendriks
H G
HG
Department of Veterinary Pathology, Faculty of Veterinary Medicine, University of Utrecht, The Netherlands.
Koninkx
J F
JF
Draaijer
M
M
van Dijk
J E
JE
Raaijmakers
J A
JA
Mouwen
J M
JM
D000818:Animals; D002417:Cattle; D007124:Immunoenzyme Techniques; D007163:Immunosorbent Techniques; D007421:Intestine, Small / Q000378:metabolism*; D037102:Lectins / Q000378:metabolism*; D008871:Microvilli / Q000378:metabolism*; D037121:Plant Lectins; D011975:Receptors, Mitogen / Q000032:analysis*; D030262:Soybean Proteins; D010835:Phytohemagglutinins
D037102:Lectins; D037121:Plant Lectins; D011975:Receptors, Mitogen; D030262:Soybean Proteins; C028824:soybean lectin; D010835:Phytohemagglutinins; C046772:soybean lectin receptor