title: DNA probes for identification of enteroinvasive Escherichia coli.
abstract: Eighty-one Escherichia coli strains belonging to all known invasive O serogroups were tested with two distinct invasiveness probes (pMR17 and pSF55). All 54 Sereny test-positive strains and 5 strains that lost Sereny positivity during storage hybridized with both probes. Probe-positive strains carried a 120- to 140-megadalton plasmid, did not produce lysine decarboxylase, and, with the exception of certain serotypes, were nonmotile. Motile strains of serotype O144:H25 were for the first time characterized as invasive by hybridization with the probes.
authors.affiliation: Department of Microbiology, Immunology, and Parasitology, Escola Paulista de Medicina, Sao Paulo, Brazil.
authors.forename: T A
authors.initials: TA
authors.lastname: Gomes
authors.forename: M R
authors.initials: MR
authors.lastname: Toledo
authors.forename: L R
authors.initials: LR
authors.lastname: Trabulsi
authors.forename: P K
authors.initials: PK
authors.lastname: Wood
authors.forename: J G
authors.initials: JG
authors.lastname: Morris
mesh_terms: D004269:DNA, Bacterial / Q000032:analysis*; D004926:Escherichia coli / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D006367:HeLa Cells; D006801:Humans; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010957:Plasmids
chemical_list: D004269:DNA, Bacterial