title: Extensive homology between the leukotoxin of Pasteurella haemolytica A1 and the alpha-hemolysin of Escherichia coli.
abstract: The 19.8- and 101.9-kilodalton leukotoxin proteins of Pasteurella haemolytica (LKTC and LKTA, respectively) share extensive homology with the HLYC and HLYA alpha-hemolysin proteins of Escherichia coli. The leukotoxin LKTA protein cross-reacts with hemolysin-specific antisera in Western blot (immunoblot) analysis, indicating that it shares epitopes with the alpha-hemolysin HLYA protein. Both LKTA and HLYA contain a conserved hydrophobic region, as well as a set of tandemly repeated domains. These features have been implicated in the lytic function of the alpha-hemolysin.
authors.affiliation: Department of Microbiology, University of Guelph, Ontario, Canada.
authors.forename: C A
authors.initials: CA
authors.lastname: Strathdee
authors.forename: R Y
authors.initials: RY
authors.lastname: Lo
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000276:immunology; D003429:Cross Reactions; D004926:Escherichia coli / Q000472:pathogenicity; D029968:Escherichia coli Proteins; D005098:Exotoxins / Q000276:immunology; D006460:Hemolysin Proteins; D008969:Molecular Sequence Data; D010325:Pasteurella / Q000472:pathogenicity*; D011487:Protein Conformation; D012995:Solubility
chemical_list: D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D005098:Exotoxins; D006460:Hemolysin Proteins; C036507:Hlya protein, E coli; C018579:leukotoxin