title: Cloning and sequencing of the genes encoding the large and small subunits of the periplasmic (NiFeSe) hydrogenase of Desulfovibrio baculatus.
abstract: The genes coding for the large and small subunits of the periplasmic hydrogenase from Desulfovibrio baculatus have been cloned and sequenced. The genes are arranged in an operon with the small subunit gene preceding the large subunit gene. The small subunit gene codes for a 32 amino acid leader sequence supporting the periplasmic localization of the protein, however no ferredoxin-like or other characteristic iron-sulfur coordination sites were observed. The periplasmic hydrogenases from D. baculatus (an NiFeSe protein) and D. vulgaris (an Fe protein) exhibit no homology suggesting that they are structurally different, unrelated entities.
authors.affiliation: Department of Biochemistry, School of Chemical Sciences, University of Georgia, Athens 30602.
authors.forename: N K
authors.initials: NK
authors.lastname: Menon
authors.forename: H D
authors.initials: HD
authors.lastname: Peck
authors.forename: J L
authors.initials: JL
authors.lastname: Gall
authors.forename: A E
authors.initials: AE
authors.lastname: Przybyla
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D003062:Codon / Q000235:genetics; D003429:Cross Reactions; D004269:DNA, Bacterial / Q000235:genetics; D003901:Desulfovibrio / Q000201:enzymology / Q000235:genetics*; D005798:Genes, Bacterial; D006864:Hydrogenase / Q000235:genetics* / Q000276:immunology; D007118:Immunoassay; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D009876:Operon; D012689:Sequence Homology, Nucleic Acid
chemical_list: D003062:Codon; D004269:DNA, Bacterial; D006864:Hydrogenase