Crystallization and preliminary X-ray and optical spectroscopic characterization of the photochemical reaction center from Rhodobacter sphaeroides strain 2.4.1.
The photochemical reaction center from Rhodobacter sphaeroides 2.4.1 has been crystallized. The crystals were obtained in a solution of beta-octylglucoside by the vapor diffusion technique using polyethylene glycol 4000 as the precipitant at 22 degrees C. The orthorhombic crystals (space group P2(1)2(1)2(1)) have cell constants a = 142.5 A, b = 136.1 A, c = 78.5 A, and diffract to 3.7 A. The crystals display pronounced linear dichroism in the carotenoid absorption spectral region.
Frank
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HA
Department of Chemistry, University of Connecticut, Storrs 06268.
Taremi
S S
SS
Knox
J R
JR
D001426:Bacterial Proteins; D045322:Photosynthetic Reaction Center Complex Proteins; D012242:Rhodobacter sphaeroides / Q000032:analysis*; D013057:Spectrum Analysis; D014961:X-Ray Diffraction
D001426:Bacterial Proteins; D045322:Photosynthetic Reaction Center Complex Proteins