[Comparison of the kinetics of the response of salivary and plasma 17-hydroxyprogesterone to the administration of HCG in men].
17-hydroxyprogesterone (17-OHP) time course response to hCG (5,000 IU) was studied simultaneously in the saliva and the plasma of 12 adult healthy men. In both fluids, 17-OHP was determined by radioimmunossay after chromatography on Sephadex LH-20 columns of diethyl ether extracts. The use of an iodinated tracer has greatly increased the sensitivity of the technique. Baseline levels in plasma and saliva were: 1.0 +/- 0.1 ng/ml (mean +/- SEM) and 24 +/- 2 pg/ml respectively. After hCG, a biphasic pattern was observed in both fluids with a similar early response but the peak elicited at 33 h in plasma was not observed in saliva where the levels were lower than those recorded at 24 h. At 48 h saliva 17-OHP increased again to levels similar to those at 24 h. Thus the 17-OHP pattern in saliva was the mirror image of that found in plasma. Since saliva steroids are believed to reflect the plasma non-protein bound fraction, this difference was assumed to be due to the decrease of the unbound fraction of plasma 17-OHP in the late afternoon as a consequence of the increase of the CBG-bound fraction since at that time cortisol levels are low. Indeed the ratios of saliva to plasma 17-OHP levels were found to be significantly correlated with plasma cortisol levels: r = 0.44 (p less than 0.01; n = 140). Thus the absence of the secondary peak at 33 h may be due to cortisol circadian rhythm. However the similar response in saliva at 24 and at 48 h after hCG was enough important to make reliable the evaluation of the endocrine testicular function so that saliva may be collected instead of plasma in the hCG stimulation test protocol.
Nahoul
K
K
Fondation de Recherche en Hormonologie, Fresnes, France.
Paysant
F
F
Gali
N
N
D019326:17-alpha-Hydroxyprogesterone; D000328:Adult; D006063:Chorionic Gonadotropin / Q000494:pharmacology*; D006801:Humans; D006908:Hydroxyprogesterones / Q000097:blood / Q000493:pharmacokinetics*; D008297:Male; D012463:Saliva / Q000378:metabolism*; D013997:Time Factors
D006063:Chorionic Gonadotropin; D006908:Hydroxyprogesterones; D019326:17-alpha-Hydroxyprogesterone
Comparaison de la cinétique de la réponse de la 17-hydroxyprogestérone salivaire et plasmatique à l'administration de hCG chez l'homme.