title: Prostatic growth factor: purification and structural relationship to basic fibroblast growth factor.
abstract: Prostatic growth factor (PrGF) was purified from alkaline homogenates of human benign prostatic hyperplastic tissue by a combination of ammonium sulfate precipitation, heparin affinity chromatography, and cation-exchange chromatography. The 17,600-dalton, basic (pI 10.2) PrGF is related to basic fibroblast growth factor (bFGF) since antisera raised against synthetic peptides with sequence homologies corresponding to an internal peptide and amino- and carboxyl-terminal peptides of bFGF react with the growth factor. The growth factor appears larger than bFGF, suggesting that additional amino-terminal sequences may be present as a result of alkaline extraction in the presence of protease inhibitors.
authors.affiliation: Department of Urology, Medical College of Wisconsin, Milwaukee 53226.
authors.forename: M T
authors.initials: MT
authors.lastname: Story
authors.forename: J
authors.initials: J
authors.lastname: Sasse
authors.forename: S C
authors.initials: SC
authors.lastname: Jacobs
authors.forename: R K
authors.initials: RK
authors.lastname: Lawson
mesh_terms: D000595:Amino Acid Sequence; D000645:Ammonium Sulfate / Q000494:pharmacology; D002846:Chromatography, Affinity / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D003101:Collodion; D004591:Electrophoresis, Polyacrylamide Gel; D005346:Fibroblast Growth Factors / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D006965:Hyperplasia; D007124:Immunoenzyme Techniques; D008297:Male; D008970:Molecular Weight; D011467:Prostate / Q000032:analysis* / Q000473:pathology; D014020:Tissue Extracts
chemical_list: D014020:Tissue Extracts; D005346:Fibroblast Growth Factors; D003101:Collodion; D000645:Ammonium Sulfate