Inhibition of hydrolytic activities of human dipeptidases toward leukotriene D4 and kyotorphin by captopril.
We investigated inhibition by captopril of the hydrolysis of leukotriene D4 (LTD4) and kyotorphin by human dipeptidases (DPases). The rate of LTD4 hydrolysis by kidney DPases F and S, pancreas and liver DPases were 60.6, 23.8, 23.3 and 0.358 units/mg protein, respectively. The inhibition by captopril was competitive for DPase F and liver DPase, and non-competitive for DPase S and pancreas enzyme. Using kyotorphin as a substrate, captopril inhibited four DPases competitively. From these results it is suggested that some side effects of captopril may relate to inhibition of hydrolytic activities of DPases with physiologically active dipeptides in man.
Ito
Y
Y
Iwaki
M
M
Ogiso
T
T
Nakamura
S
S
Kato
K
K
Sawaki
S
S
D000700:Analgesics / Q000378:metabolism*; D002216:Captopril / Q000494:pharmacology*; D004150:Dipeptidases / Q000037:antagonists & inhibitors*; D004723:Endorphins / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D013189:SRS-A / Q000378:metabolism*; D013379:Substrate Specificity
D000700:Analgesics; D004723:Endorphins; D013189:SRS-A; C019448:kyotorphin; D002216:Captopril; D004150:Dipeptidases