Electrostatic and hydrophobic interactions of the intermediate filament protein vimentin and its amino terminus with lipid bilayers.
Immunofluorescence and electron microscopical studies on the intracellular distribution of intermediate filaments (IFs) have demonstrated a close proximity of these cytoskeletal structures to cellular membranes. Moreover, nonepithelial IF (protein)s have been shown to exhibit high affinities for lipids, especially for negatively charged and nonpolar lipids. Here, using hydrophobic labeling with the photoactivatable phosphatidylcholine analogue [3H]1-palmitoyl-2-[11-[4-(trifluoromethyldiazirinyl]undecanoyl+ ++]-sn- glycero-3-phosphorylcholine or with 1-azidopyrene at low and physiological ionic strength, it is demonstrated that the IF subunit protein vimentin can interact with the hydrophobic core of lipid bilayers, in addition to strong ionic relationships between both reactants. Whereas the presence of acidic phospholipids in the lipid vesicles was absolutely essential for efficient vimentin labeling, cholesterol played a synergistic role in this reaction. Proteolytic degradation of photolabeled vimentin localized the derivatization exclusively to the non-alpha-helical, highly positively charged N-terminal domain of the filament protein. Furthermore, circular dichroism studies performed on the isolated N terminus of vimentin revealed a significant increase in the alpha-helical content of the polypeptide upon its interaction with vesicles containing negatively charged phospholipids. These results indicate an amphiphilic character of the N terminus and suggest that the cationic arginine residues of the N-terminal domain react with the negatively charged head groups of acidic phospholipids prior or parallel to interaction of the polypeptide with hydrophobic regions of the lipid bilayer.
Perides
G
G
Max-Planck-Institut für Zellbiologie, Ladenburg/Heidelberg, Federal Republic of Germany.
Harter
C
C
Traub
P
P
D000345:Affinity Labels / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001389:Azirines / Q000378:metabolism; D002784:Cholesterol / Q000494:pharmacology; D006224:Cricetinae; D005455:Fluorescent Antibody Technique; D007700:Kinetics; D008051:Lipid Bilayers; D051379:Mice; D009994:Osmolar Concentration; D010446:Peptide Fragments / Q000378:metabolism; D010713:Phosphatidylcholines / Q000378:metabolism; D014746:Vimentin / Q000378:metabolism*
D000345:Affinity Labels; D001389:Azirines; D008051:Lipid Bilayers; D010446:Peptide Fragments; D010713:Phosphatidylcholines; D014746:Vimentin; C048903:1-palmitoyl-2-(10-(4-((trifluoro-methyl)diazirinyl)phenyl)-9-oxaundecanoyl)-sn-glycero-3-phosphocholine; D002784:Cholesterol