title: Hydrogenase activity in aged, nonviable Desulfovibrio vulgaris cultures and its significance in anaerobic biocorrosion.
abstract: Batch cultures of Desulfovibrio vulgaris stored at 32 degrees C for 10 months have been found to retain 50% of the hydrogenase activity of a 1-day culture. The hydrogenase found in old cultures needs reducing conditions for its activation. Viable cell counts are negative after 6 months, showing that the hydrogenase activity does not depend on the presence of viable cells. These observations are of importance in the understanding of anaerobic biocorrosion of metals caused by depolarization phenomena.
authors.affiliation: Section d'Enzymologie et Biochimie Bactérienne, Association pour la Recherche en Bioénergie Solaire, CEN Cadarache, Saint Paul Lez Durance, France.
authors.forename: C
authors.initials: C
authors.lastname: Chatelus
authors.forename: P
authors.initials: P
authors.lastname: Carrier
authors.forename: P
authors.initials: P
authors.lastname: Saignes
authors.forename: M F
authors.initials: MF
authors.lastname: Libert
authors.forename: Y
authors.initials: Y
authors.lastname: Berlier
authors.forename: P A
authors.initials: PA
authors.lastname: Lespinat
authors.forename: G
authors.initials: G
authors.lastname: Fauque
authors.forename: J
authors.initials: J
authors.lastname: Legall
mesh_terms: D000693:Anaerobiosis; D003343:Corrosion; D003901:Desulfovibrio / Q000201:enzymology* / Q000254:growth & development; D006864:Hydrogenase / Q000378:metabolism*; D007700:Kinetics; D008670:Metals / Q000378:metabolism*
chemical_list: D008670:Metals; D006864:Hydrogenase