[Modulation of graft versus host disease by in vitro incubation of donor cells with deoxycoformycin and deoxyadenosine].
The combination of deoxycoformycin and deoxyadenosine was investigated for its capability to deplete T-cells from bone marrow and spleen cells and for its effect on GVHD in MHC-mismatched transplantation in rats. In vitro incubation with DCF/dADO for 18-20 hours resulted in significant but incomplete T-cell depletion without toxicity towards CFU-M. This corresponded with a lower incidence and a modification of GVHD following transplantation of such treated cells into MHC-incompatible recipient rats. However, GVHD could not be completely prevented by the in vitro treatment of donor cells.
Schofer
O
O
Kinderklinik und Kinder-Poliklinik, Universität Mainz.
Veit
B C
BC
D000818:Animals; D016026:Bone Marrow Transplantation; D003070:Coformycin / Q000008:administration & dosage* / Q000031:analogs & derivatives; D003839:Deoxyadenosines / Q000008:administration & dosage*; D004305:Dose-Response Relationship, Drug; D006086:Graft vs Host Disease / Q000517:prevention & control*; D007166:Immunosuppressive Agents / Q000008:administration & dosage*; D015649:Pentostatin; D051381:Rats; D012263:Ribonucleosides / Q000008:administration & dosage*; D013154:Spleen / Q000637:transplantation*; D013601:T-Lymphocytes / Q000187:drug effects
D003839:Deoxyadenosines; D007166:Immunosuppressive Agents; D012263:Ribonucleosides; D003070:Coformycin; D015649:Pentostatin
Modulation der Graft-versus-host-Erkrankung durch In-vitro-Inkubation von Spenderzellen mit Deoxycoformycin und Deoxyadenosin.