An endothelial cell growth factor derived from human lung carcinoma cells grown in serum-free medium.
A factor that stimulates the proliferation of human umbilical vein endothelial cells has been shown to be present in serum-free medium conditioned by the prior growth of a cell line (1PT) derived from a poorly differentiated bronchial carcinoma. Preliminary characterization of this factor has revealed that it is a heat-labile, acid-stable proteinaceous material, the activity of which is not diminished by treatment with a reducing agent. In its partially purified state it has been shown to be anionic and to be associated with material exhibiting a broad molecular weight range of 35 X 10(3) to 100 X 10(3). It does not bind strongly to heparin-Sepharose and its mitogenic effect on endothelial cells is not potentiated by heparin. These properties suggest that this factor may differ from other previously described tumour-derived endothelial mitogens.
Walker
C
C
F.K. Douglas Cancer Research Laboratories, Clatterbridge Hospital, Bebington, Merseyside, UK.
Mates
G
G
Pumford
D
D
Daniel
M
M
D002283:Carcinoma, Bronchogenic / Q000378:metabolism*; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D016228:Endothelial Growth Factors; D004727:Endothelium / Q000166:cytology / Q000187:drug effects; D006133:Growth Substances / Q000302:isolation & purification* / Q000494:pharmacology; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism*
D016228:Endothelial Growth Factors; D006133:Growth Substances