Neural-specific carbohydrate moiety shared by many surface glycoproteins in Drosophila and grasshopper embryos.
Antiserum against horseradish peroxidase (anti-HRP Ab) labels the surfaces of neurons in both Drosophila and grasshopper (Jan and Jan, 1982). Here we show that the anti-HRP Ab (1) immunoprecipitates at least 17 different membrane glycoproteins from the Drosophila embryo CNS (and a similar array from grasshopper), and (2) recognizes a neural-specific carbohydrate moiety expressed by most if not all of these proteins. Although the anti-HRP Ab stains all axon pathways, 2 of the anti-HRP glycoproteins, fasciclin I and II, are expressed on specific subsets of axon pathways in the grasshopper embryo.
Snow
P M
PM
Department of Biological Sciences, Stanford University, California 94305.
Patel
N H
NH
Harrelson
A L
AL
Goodman
C S
CS
D000818:Animals; D000906:Antibodies; D002241:Carbohydrates / Q000032:analysis*; D015816:Cell Adhesion Molecules, Neuronal; D004330:Drosophila; D004625:Embryo, Nonmammalian; D005455:Fluorescent Antibody Technique; D006023:Glycoproteins / Q000032:analysis*; D006110:Grasshoppers; D006735:Horseradish Peroxidase; D008562:Membrane Glycoproteins / Q000032:analysis; D009474:Neurons / Q000032:analysis*
D000906:Antibodies; D002241:Carbohydrates; D015816:Cell Adhesion Molecules, Neuronal; D006023:Glycoproteins; D008562:Membrane Glycoproteins; C051641:fasciclin I; C051642:fasciclin II; D006735:Horseradish Peroxidase