Nonsense suppression context effects in Escherichia coli bacteriophage T4.
Nonsense suppression by supE44 has been examined in a collection of 14 T4 gene 22 and gene 23 UAG mutants, for which the precise gene location is known. In concordance with previous studies, UAG followed by a pyrimidine was inefficiently suppressed. However, among positions with similar 3' nucleotides, there was considerable variation in suppression efficiency. The competition between supE44 and Release Factor 1 (RF 1) was also investigated following the introduction of a multicopy RF 1 plasmid. An inverse relationship between the efficiency of suppression and RF 1 competition was observed.
Edelmann
P
P
Martin
R
R
Gallant
J
J
D003062:Codon; D004926:Escherichia coli / Q000235:genetics*; D005814:Genes, Viral; D005838:Genotype; D013489:Suppression, Genetic; D013604:T-Phages / Q000235:genetics*
D003062:Codon