Crosslinking of elongation factor Tu to tRNA(Phe) by trans-diamminedichloroplatinum (II). Characterization of two crosslinking sites in the tRNA.
Trans-diamminedichloroplatinum (II) was used to induce reversible crosslinks between EF-Tu and Phe-tRNA(Phe) within the ternary EF-Tu/GTP/Phe-tRNA(Phe) complex. Up to 40% of the complex was specifically converted into crosslinked species. Two crosslinking sites have been unambiguously identified. The major one encompassing nucleotides 58 to 65 is located in the 3'-part of the T-stem, and the minor one encompassing nucleotides 31 to 42 includes the anticodon loop and part of the 3'-strand of the anticodon stem.
Wikman
F P
FP
Romby
P
P
Metz
M H
MH
Reinbolt
J
J
Clark
B F
BF
Ebel
J P
JP
Ehresmann
C
C
Ehresmann
B
B
D001483:Base Sequence; D002945:Cisplatin / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D006160:Guanosine Triphosphate / Q000378:metabolism; D007536:Isomerism; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D010444:Peptide Elongation Factor Tu / Q000302:isolation & purification / Q000378:metabolism*; D012346:RNA, Transfer, Amino Acyl / Q000302:isolation & purification / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism
D012346:RNA, Transfer, Amino Acyl; C034697:transplatin; D006160:Guanosine Triphosphate; D010444:Peptide Elongation Factor Tu; D002945:Cisplatin