title: Comparative mutational analysis of wild-type and stretched tRNA3(Leu) gene promoters.
abstract: We demonstrate that, when the yeast tRNA(3Leu) gene is stretched so that the distance between the two portions of the intragenic promoter is increased to 365 base pairs, the A and B blocks remain functional. Mutations in the A block, which show a weak phenotype when inserted in the wild type, exert a dramatic effect when inserted into the stretched gene. Experiments with extensively purified transcription factor tau indicate that the tau B-B block interaction is not influenced by A-B distance; only the ability of tau A to interact with A block sequences is affected, possibly because of the additional free-energy cost of forming a large loop of the intervening DNA.
authors.affiliation: Dipartimento di Biologia Cellulare e dello Sviluppo, Università degli Studi La Sapienza, Rome, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Fabrizio
authors.forename: A
authors.initials: A
authors.lastname: Coppo
authors.forename: P
authors.initials: P
authors.lastname: Fruscoloni
authors.forename: P
authors.initials: P
authors.lastname: Benedetti
authors.forename: G
authors.initials: G
authors.lastname: Di Segni
authors.forename: G P
authors.initials: GP
authors.lastname: Tocchini-Valentini
mesh_terms: D001483:Base Sequence; D001665:Binding Sites; D004252:DNA Mutational Analysis; D004271:DNA, Fungal / Q000235:genetics; D004268:DNA-Binding Proteins / Q000502:physiology; D005786:Gene Expression Regulation; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012345:RNA, Transfer, Amino Acid-Specific / Q000235:genetics*; D012356:RNA, Transfer, Leu / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014157:Transcription Factors / Q000502:physiology*; D014158:Transcription, Genetic
chemical_list: D004271:DNA, Fungal; D004268:DNA-Binding Proteins; D012345:RNA, Transfer, Amino Acid-Specific; D012356:RNA, Transfer, Leu; D014157:Transcription Factors