Improvement of transplantability of human neoplastic cells to nude mice.
It was demonstrated from these results that both splenectomy and irradiation of the nude mice are effective procedures for improving the rate of successful xenotransplantation with nude mice. When there is only a small number of cells in the transplant material, applying only one treatment of either splenectomy or irradiation is not sufficient to effectively improve the rate of successful transplantation. A combination of both treatments was more effective for increasing the successful rate of transplantation. The optimal conditions for maximizing the successful transplantation rate from this study were splenectomy on the 4th day of age and irradiation and transplantation on the 35th day of age for the xenotransplantation of a small number of neoplastic cells.
Sudo
K
K
Laboratory Animal Research Center, University of Tokyo, Japan.
D000818:Animals; D002277:Carcinoma / Q000276:immunology; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D013156:Splenectomy; D014407:Tumor Cells, Cultured; D014916:Whole-Body Irradiation