Demonstration of glucose inhibition of insulin release in the presence of diazoxide.
beta-Cell-rich pancreatic islets from ob/ob mice were taken for measurements of insulin release in response to glucose after culture in RPMI 1640 medium. The stimulatory effect of 20 mmol/l glucose was converted into an inhibition when the medium was supplemented with 400 mumol/l diazoxide. Glucose inhibition of insulin release was observed when the islets had been cultured in the presence of 1 or 20 mmol/l glucose in media either containing or lacking Ca2+. The data provide further evidence for an inhibitory component in the action of glucose on insulin release, suggesting that glucose stimulation of the Ca2+ efflux is essential for the appearance of this inhibition.
Bergsten
P
P
Hellman
B
B
D000818:Animals; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D003470:Culture Media; D003981:Diazoxide / Q000494:pharmacology*; D005947:Glucose / Q000494:pharmacology*; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000378:metabolism*; D051379:Mice; D008820:Mice, Obese
D003470:Culture Media; D007328:Insulin; D005947:Glucose; D003981:Diazoxide; D002118:Calcium