Effect of freezing on lens epithelial cell growth.
The effect of freezing on the growth of rat lens epithelial cells was studied in vitro. We found that 80% of the lens epithelial cells died after freezing at -45 degrees C for two hours and that the surviving cells could grow with the addition of growth factors or when placed on a sheet of type 4 collagen, but not when placed on a plain plastic culture dish. These results suggest that the surviving cells are at the Go phase of the cell cycle and that type 4 collagen or growth factors can initiate cell division.
Fukaya
Y
Y
Department of Biophysical Chemistry, Faculty of Pharmaceutical Sciences, Meijo University, Nagoya, Japan.
Hara
T
T
Hara
T
T
Iwata
S
S
D000818:Animals; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D003094:Collagen / Q000145:classification / Q000494:pharmacology; D004815:Epidermal Growth Factor / Q000494:pharmacology; D004847:Epithelial Cells; D005615:Freezing; D007328:Insulin / Q000494:pharmacology; D007908:Lens, Crystalline / Q000166:cytology*; D013997:Time Factors
D007328:Insulin; D004815:Epidermal Growth Factor; D003094:Collagen