title: Expression of human adenosine deaminase in nonhuman primates after retrovirus-mediated gene transfer.
abstract: Primate bone marrow cells were infected with a retroviral vector carrying the genes for human adenosine deaminase (h-ADA) and bacterial neomycin resistance (neor). The infected cells were infused back into the lethally irradiated donor animals. Several monkeys fully reconstituted and were shown to express the h-ADA and neor genes at low levels in their recirculating hematopoietic cells for short periods of time.
authors.forename: P W
authors.initials: PW
authors.lastname: Kantoff
authors.forename: A P
authors.initials: AP
authors.lastname: Gillio
authors.forename: J R
authors.initials: JR
authors.lastname: McLachlin
authors.forename: C
authors.initials: C
authors.lastname: Bordignon
authors.forename: M A
authors.initials: MA
authors.lastname: Eglitis
authors.forename: N A
authors.initials: NA
authors.lastname: Kernan
authors.forename: R C
authors.initials: RC
authors.lastname: Moen
authors.forename: D B
authors.initials: DB
authors.lastname: Kohn
authors.forename: S F
authors.initials: SF
authors.lastname: Yu
authors.forename: E
authors.initials: E
authors.lastname: Karson
mesh_terms: D000243:Adenosine Deaminase / Q000235:genetics*; D000818:Animals; D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation; D004247:DNA / Q000235:genetics; D004351:Drug Resistance / Q000235:genetics; D006412:Hematopoietic Stem Cells / Q000201:enzymology; D006801:Humans; D008214:Lymphocytes / Q000201:enzymology; D008252:Macaca fascicularis; D009000:Monocytes / Q000201:enzymology; D009355:Neomycin; D009693:Nucleic Acid Hybridization; D009700:Nucleoside Deaminases / Q000235:genetics*; D012190:Retroviridae / Q000235:genetics*; D014170:Transformation, Genetic; D014916:Whole-Body Irradiation
chemical_list: D004247:DNA; D009700:Nucleoside Deaminases; D000243:Adenosine Deaminase; D009355:Neomycin