Polyamine content as a marker of radiation injury in the rat spleen.
The modifications of the polyamines putrescine (put), spermidine (spd) and spermine (spm) in rat spleen after 3 Gy whole-body irradiation were studied. Rats were irradiated at four different times of the day (00.00, 06.00, 12.00 and 18.00) and sacrificed between 12 h and 62 days after irradiation. Control animals, sacrificed at the same times of the day, showed higher levels of the spd/spm ratio during the hours of light. After irradiation the polyamine content was rapidly and significantly reduced over a period of 20 days. The modification of the amount of spm lasted for a longer period of time. Normal values of polyamine content were reached at later times when the mitotic activity was restored. The results show a close correlation between polyamine concentration and [3H]thymidine uptake.
Becciolini
A
A
Department of Clinical Physiopathology, University of Florence, Italy.
Porciani
S
S
Lanini
A
A
Balzi
M
M
D000818:Animals; D003037:Cobalt Radioisotopes; D005260:Female; D005720:Gamma Rays; D011073:Polyamines / Q000378:metabolism*; D011700:Putrescine / Q000378:metabolism; D011833:Radiation Injuries, Experimental / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013095:Spermidine / Q000378:metabolism; D013096:Spermine / Q000378:metabolism; D013154:Spleen / Q000378:metabolism / Q000528:radiation effects*; D014916:Whole-Body Irradiation
D003037:Cobalt Radioisotopes; D011073:Polyamines; D013096:Spermine; D013095:Spermidine; D011700:Putrescine