Functional expression of the cDNA encoding for human lactate dehydrogenase-A in Chinese hamster ovary cells.
The cloned cDNA encoding for human lactate dehydrogenase-A was inserted immediately downstream to the SV40 early promoter, and it was shown to synthesize the human LDH-A polypeptide in Chinese hamster ovary cells. The human LDH-A subunit and the endogenous Chinese hamster LDH-a subunit formed in vivo a heterotetrameric LDH-a3A1 functional isoenzyme, indicating the conserved tertiary structure of both LDH-A subunits.
Hou
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Laboratory of Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709.
Li
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D000818:Animals; D002478:Cells, Cultured; D003001:Cloning, Molecular; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000235:genetics*; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000096:biosynthesis / Q000235:genetics*; D010053:Ovary; D011487:Protein Conformation
D007527:Isoenzymes; D004247:DNA; D007770:L-Lactate Dehydrogenase