[Mutants of the yeast Saccharomyces cerevisiae characterized by enhanced induced mutagenesis. III. Effect of the him mutation on the effectiveness and specificity of UF-induced mutagenesis].
We have studied the influence of him1-1, him2-1, him3-1 and himX mutations on induction frequency and specificity of UV-induced adenine-dependent mutations in the yeast Saccharomyces cerevisiae. Him mutations do not render haploid cells more sensitive to the lethal action of UV-light; however, in him strains adenine-dependent mutations (ade1, ade2) were induced more frequently (1.5--2-fold), as compared to the HIM strain. An analysis of the molecular nature of ade2 mutants revealed that him1-1, him2-1 and himX mutations increase specifically the yield of transitions (AT----GC and GC----AT), whereas in the him3-1 strain the yield of transversions was enhanced as well. We suggest him mutations analysed to affect specific repair pathway for mismatch correction.
Ivanov
E L
EL
Koval'tsova
S V
SV
Korolev
V G
VG
D005800:Genes, Fungal; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000528:radiation effects; D013489:Suppression, Genetic; D014466:Ultraviolet Rays
Mutanty drozhzheĭ Saccharomyces cerevisiae, kharakterizuiushchiesia povyshennym indutsirovannym mutagenezom. Soobshchenie III. Vliianie mutatsii him na éffektivnost' i spetsifichnost' UF-indutsirovannogo mutageneza.