Nicotinic receptor stimulation enhances enkephalin-like peptides processing in chromaffin cells.
Acute stimulation of chromaffin cells in cultures with acetylcholine (ACh), 1,1-dimethyl-4-phenylpiperazinium (DMPP), or high potassium gave rise to a significant increase in the release of [Met5]-enkephalin immunoreactive material (ME-IRM) into the assay medium. The cellular content of ME-IRM following the actual release induced by these secretagogues remained constant suggesting the replenishment of the cellular peptides. The repletion of the peptides may occur through an enhancement of the processing rate of the proenkephalin precursor. Furthermore, the increase in secretion as well as the repletion of the cellular ME-IRM were calcium-dependent and were inhibited by the nicotinic receptor antagonist, hexamethonium, but not by atropine. These results indicate that secretion and repletion of the peptides are tightly coupled and activated by nicotinic receptor stimulation.
Cherdchu
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Department of Pharmacology, University of Nebraska Medical Center, Omaha 68105.
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D000109:Acetylcholine / Q000494:pharmacology; D000313:Adrenal Medulla / Q000187:drug effects / Q000502:physiology*; D000818:Animals; D043424:Carboxypeptidase B; D002268:Carboxypeptidases; D002417:Cattle; D002838:Chromaffin System / Q000187:drug effects / Q000502:physiology*; D004246:Dimethylphenylpiperazinium Iodide / Q000494:pharmacology; D004744:Enkephalin, Methionine / Q000378:metabolism; D004745:Enkephalins / Q000378:metabolism*; D016210:Methacholine Chloride; D008688:Methacholine Compounds / Q000494:pharmacology; D011188:Potassium / Q000494:pharmacology; D011498:Protein Precursors / Q000378:metabolism; D011863:Radioimmunoassay; D011978:Receptors, Nicotinic / Q000187:drug effects / Q000502:physiology*; D014357:Trypsin
D004745:Enkephalins; D008688:Methacholine Compounds; D011498:Protein Precursors; D011978:Receptors, Nicotinic; C029992:proenkephalin; D016210:Methacholine Chloride; D004246:Dimethylphenylpiperazinium Iodide; D004744:Enkephalin, Methionine; D002268:Carboxypeptidases; D043424:Carboxypeptidase B; D014357:Trypsin; D000109:Acetylcholine; D011188:Potassium