title: Mode of action of intercalating drug, cis-Pt(II)(DDH)Cl2, cis-Pt(II)(DDH) (metafluorobenzoic)2, and cis-Pt(II)(DDH)(mucubromic)2, on Trypanosoma cruzi.
abstract: The trypanocidal activity of three new compounds derived of the antineoplastic drug cis-diamminodichloroplatinum(II) (DDP) has been studied over epimastigote forms of Trypanosoma cruzi. The electron microscopy indicated that, in the treated animals, the nuclear chromatin is distributed in the nucleus in a lax form and that the number of lipidic vacuoles increases largely. This action is more significant when cis-Pt(II)1,2-diaminocyclohexane is used. In addition this compound causes a destabilization of the DNA double helix with a decrease of melting temperature. The three compounds inhibit the incorporation of leucine, uridine and thymidine, although the thymidine uptake is the most significantly affected.
authors.forename: L M
authors.initials: LM
authors.lastname: Ruiz-Perez
authors.forename: A
authors.initials: A
authors.lastname: Osuna
authors.forename: M C
authors.initials: MC
authors.lastname: Lopez
authors.forename: S
authors.initials: S
authors.lastname: Castanys
authors.forename: F
authors.initials: F
authors.lastname: Gammaro
authors.forename: D
authors.initials: D
authors.lastname: Craciunescu
authors.forename: C
authors.initials: C
authors.lastname: Alonso
mesh_terms: D000818:Animals; D008854:Microscopy, Electron; D009944:Organoplatinum Compounds / Q000494:pharmacology*; D014344:Trypanocidal Agents / Q000494:pharmacology*; D014349:Trypanosoma cruzi / Q000187:drug effects* / Q000648:ultrastructure
chemical_list: D009944:Organoplatinum Compounds; D014344:Trypanocidal Agents; C052583:cis-Pt(II)(DDH)bis(mucobromic acid); C013933:dichloro-1,2-diaminocyclohexane platinum complex; C052582:cis-Pt(II)(DDH)bis(metafluorobenzoic acid)