Mapping of the constitutive lysyl-tRNA synthetase gene of Escherichia coli K-12.
The constitutive lysyl-tRNA synthetase gene (lysS) was mapped at 62.1 min on the Escherichia coli chromosome by a combination of conjugation and transduction, with physical confirmation by two-dimensional gel electrophoresis. Revertant analysis suggests that the altered isoelectric point and the low amount of the mutant LysS protein may be due to a single mutational event.
Emmerich
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RV
Department of Biological Sciences, St. John's University, Jamaica, New York 11439.
Hirshfield
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IN
D000604:Amino Acyl-tRNA Synthetases / Q000235:genetics*; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial / Q000502:physiology; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005796:Genes; D005798:Genes, Bacterial; D005838:Genotype; D008250:Lysine-tRNA Ligase / Q000235:genetics*; D010641:Phenotype
D000604:Amino Acyl-tRNA Synthetases; D008250:Lysine-tRNA Ligase