[Identification of idiotrophs for 3'-deoxykanamycin B synthesis in Streptomyces tenebrarius (Higgens a. Kastner)].
Mutants of Streptomyces tenebrarius with the blocked synthesis of 3'-deoxykanamycin B were obtained by treating the producer with NTG and chloramphenicol, or after gamma-irradiation. These mutants (idiotrophs) were distributed into three groups by means of the cosynthesis experiments on agar plates: convertors, secretors and "neutral" strains. Five idiotrophs represented five complementation groups for biosynthesis of the antibiotic. Three of these were defective in 2-deoxystreptamine synthesis, the fourth was defined as neamine-negative, and the fifth was probably blocked in regulation of enzymes responsible for conversion of neamine or paromamine into kanamycins. Localization of mutations has been shown on the scheme of kanamycins' biosynthesis.
Vikhanskiĭ
Iu D
IuD
Zhdanovich
Iu V
IuV
Bartoshevich
Iu E
IuE
D001431:Bacteriological Techniques; D007612:Kanamycin / Q000031:analogs & derivatives / Q000096:biosynthesis; D009154:Mutation; D013302:Streptomyces / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D014031:Tobramycin / Q000096:biosynthesis
C001495:bekanamycin; D007612:Kanamycin; D014031:Tobramycin
Identifikatsiia idiotrofov po sintezu 3'-dezoksikanamitsina B u Streptomyces tenebrarius (Higgens a. Kastner).