In vitro transcripts of a yeast variant 5 S rRNA gene exhibit alterations in 3'-end processing and protein binding.
When a yeast 5 S rRNA gene with a variant 3'-terminal nucleotide sequence was transcribed in a homologous cell-free system, primary transcripts that were shorter, more heterogeneous in length and much more resistant to processing than pre-5 S rRNA from the major "repeat" 5 S rRNA gene were produced. After 3'-end processing, variant 5 S rRNA was one nucleotide shorter than mature repeat 5 S rRNA and was bound much less strongly to ribosomal protein YL3. The stable variant pre-5 S rRNA appeared to bind another, unidentified protein. The formation of this presumptive ribonucleoprotein complex was disrupted by (Up)4UOH, suggesting that it may contain a yeast analog of the mammalian La protein.
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Department of Biology, University of California, San Diego, La Jolla 92093.
D001483:Base Sequence; D004275:DNA, Ribosomal / Q000235:genetics; D005800:Genes, Fungal; D014644:Genetic Variation; D010957:Plasmids; D011485:Protein Binding; D012323:RNA Processing, Post-Transcriptional; D012335:RNA, Ribosomal / Q000235:genetics*; D012341:RNA, Ribosomal, 5S / Q000235:genetics* / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014158:Transcription, Genetic
D004275:DNA, Ribosomal; D012335:RNA, Ribosomal; D012341:RNA, Ribosomal, 5S