Immunoelectron microscopy pinpoints glutathione-peroxidase (GSH-PO) in neonatal rat adrenal cortical cells.
Localization of glutathione-peroxidase (GSH-PO) in adrenal cortical cells of neonatal rats were determined by immunocytochemical analysis. GSH-PO first appeared 14 days after birth. Intracellular localization of GSH-PO was mainly in cytosol (cytosol GSH-PO) but no intramitochondrial localization of GSH-PO was detected. Twenty-one days after birth, intramitochondrial localization of GSH-PO (mitochondrial GSH-PO) was present. Mitochondrial GSH-PO depends on ACTH stimulation; therefore, its presence in the adrenal cortical cells of neonatal rats may be the morphologic expression of the gradual acquisition of adult metabolic features during steroidogenesis. The intracellular GSH-PO staining pattern in adrenal cortical cells therefore should be a useful marker for steroidogenesis.
Murakoshi
M
M
Osamura
Y
Y
Watanabe
K
K
D000302:Adrenal Cortex / Q000201:enzymology* / Q000254:growth & development / Q000648:ultrastructure; D000375:Aging; D000818:Animals; D000831:Animals, Newborn; D003600:Cytosol / Q000201:enzymology / Q000648:ultrastructure; D005979:Glutathione Peroxidase / Q000378:metabolism*; D006651:Histocytochemistry; D007124:Immunoenzyme Techniques; D008297:Male; D008854:Microscopy, Electron; D008928:Mitochondria / Q000201:enzymology / Q000648:ultrastructure; D051381:Rats; D011919:Rats, Inbred Strains
D005979:Glutathione Peroxidase