title: The isolation and characterization of Ni2+ resistant mutants of Saccharomyces cerevisiae.
abstract: Four Ni2+ resistant mutants of Saccharomyces cerevisiae have been isolated and characterized. In the accumulation of Ni2+, there was no significant difference between the wild type strain and the Ni2+ resistant mutants. The synthesis of RNA in the yeast was severely inhibited by Ni2+ while inhibition of protein synthesis was less pronounced. The inhibitory effect of Ni2+ on both RNA and protein synthesis in Ni2+ resistant mutants of yeast was less pronounced than in the wild type strain, while four Ni2+ resistant mutants were more sensitive to Cd2+ and Cu2+ than was the wild type strain. Ni2+ resistant mechanism(s) appeared to exist within the cells and differed from those of Cd2+ or Cu2+.
authors.affiliation: Department of Biology, Faculty of Science, Ehime University, Matsuyama, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Joho
authors.forename: Y
authors.initials: Y
authors.lastname: Imada
authors.forename: T
authors.initials: T
authors.lastname: Murayama
mesh_terms: D002104:Cadmium / Q000494:pharmacology; D019256:Cadmium Chloride; D003035:Cobalt / Q000494:pharmacology; D003300:Copper / Q000494:pharmacology; D019327:Copper Sulfate; D004352:Drug Resistance, Microbial; D005656:Fungal Proteins / Q000096:biosynthesis; D009154:Mutation; D009532:Nickel / Q000378:metabolism / Q000494:pharmacology*; D012331:RNA, Fungal / Q000096:biosynthesis*; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D005656:Fungal Proteins; D012331:RNA, Fungal; D002104:Cadmium; D003035:Cobalt; D003300:Copper; D009532:Nickel; C018021:cobaltous chloride; D019256:Cadmium Chloride; D019327:Copper Sulfate