Induction of S-100b (beta beta) protein in human teratocarcinoma cells.
Human teratocarcinoma NT2/D1 cells undergo differentiation into a variety of cell types, including neurons, treated with retinoic acid. In the present study, the concentrations of alpha S-100 and beta S-100 proteins (alpha and beta subunits of S-100 proteins), and three subunits (alpha, beta and gamma) of enolase in NT2/D1 cells were measured using the sensitive enzyme immunoassay method. The concentration of beta S-100 was markedly increased in the cells after treatment with retinoic acid, whereas the concentration of alpha S-100 was undetectably low, indicating that the S-100b (beta beta) protein was induced by retinoic acid. On the other hand, the concentrations of the three forms of enolase isozymes did not change in the same culture. The induction of S-100b protein was not observed in the NT2/D1 cells after treatment with forskolin, dibutyryl cyclic AMP or cholera toxin. The indirect double-labeled immunofluorescence, using antibodies specific to beta S-100 and monoclonal antibodies specific to neurofilaments, revealed that both the S-100b protein and the neurofilaments were induced in the same subpopulation of cells which underwent neuronal differentiation.
Tsutsui
Y
Y
Department of Morphology, Institute for Developmental Research, Aichi Prefectural Colony, Japan.
Nogami
T
T
Sano
M
M
Kashiwai
A
A
Kato
K
K
D000911:Antibodies, Monoclonal / Q000276:immunology; D015415:Biomarkers; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D005455:Fluorescent Antibody Technique; D006801:Humans; D007118:Immunoassay; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000032:analysis; D009414:Nerve Growth Factors; D009417:Nerve Tissue / Q000187:drug effects / Q000254:growth & development; D010751:Phosphopyruvate Hydratase / Q000032:analysis; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins / Q000276:immunology / Q000378:metabolism*; D013724:Teratoma / Q000201:enzymology / Q000378:metabolism*; D014212:Tretinoin / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D015415:Biomarkers; D007527:Isoenzymes; D009414:Nerve Growth Factors; C030564:S-100 calcium-binding protein alpha subunit; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins; D014212:Tretinoin; D010751:Phosphopyruvate Hydratase