title: Synthesis and use of peptide substrates for the isolation and characterization of proenkephalin processing enzymes.
abstract: Classical methods of peptide synthesis in solution were used for the preparation of the two tetrapeptides alanyl-lysyl-arginyl-tyrosine and acetyl-alanyl-lysyl-arginyl-tyrosine-(N-methylamide). The two compounds were able to be recognized as substrates by proenkephalin processing enzymes and were used for the development of a quantitative assay for these enzymes. The first substrate proved to be convenient, although it was also partially degraded by amino- and carboxypeptidases under the conditions of the assay. The second was found to be hydrolyzed by the endopeptidases at too slow a rate to allow its routine use in the assay.
authors.forename: G
authors.initials: G
authors.lastname: Maret
authors.forename: D
authors.initials: D
authors.lastname: Blanot
authors.forename: M
authors.initials: M
authors.lastname: Welti
authors.forename: J L
authors.initials: JL
authors.lastname: Fauchere
mesh_terms: D000313:Adrenal Medulla / Q000201:enzymology; D000818:Animals; D002417:Cattle; D002837:Chromaffin Granules / Q000201:enzymology; D004745:Enkephalins / Q000235:genetics*; D007202:Indicators and Reagents; D009842:Oligopeptides / Q000138:chemical synthesis*; D010447:Peptide Hydrolases / Q000378:metabolism*; D011498:Protein Precursors / Q000235:genetics*; D011499:Protein Processing, Post-Translational; D013379:Substrate Specificity
chemical_list: D004745:Enkephalins; D007202:Indicators and Reagents; D009842:Oligopeptides; D011498:Protein Precursors; C029992:proenkephalin; D010447:Peptide Hydrolases