Electron microscopy and biochemical properties of polyamine-compacted DNA.
We have obtained polyamine-compacted DNA and analyzed it by electron microscopy employing the method described by Dubochet, suitable for the study of complexes in which the main interactions are of ionic character. In addition, we have developed a simple biochemical method, based on the action of pancreatic DNase I, to demonstrate the condensation of DNA with spermidine. DNA-spermidine complexes are resistant to the action of DNase I, and there is a strong correlation between the presence of condensed DNA forms, both as toroids and as cylinders, and the insensitivity to DNase I activity. We have also shown that pBR322 DNA-spermidine complexes are transcriptionally active in the presence of Escherichia coli RNA polymerase. This supports the data concerning the biological activity of spermidine-condensed DNA.
Baeza
I
I
Department of Biochemistry, Escuela Nacional de Ciencias Biologicas del IPN, Mexico, DF.
Gariglio
P
P
Rangel
L M
LM
Chavez
P
P
Cervantes
L
L
Arguello
C
C
Wong
C
C
Montañez
C
C
D004247:DNA / Q000031:analogs & derivatives*; D004269:DNA, Bacterial; D004926:Escherichia coli / Q000235:genetics; D007700:Kinetics; D008854:Microscopy, Electron; D009690:Nucleic Acid Conformation; D010957:Plasmids; D013095:Spermidine / Q000031:analogs & derivatives*; D014158:Transcription, Genetic
D004269:DNA, Bacterial; C007545:DNA-spermidine; D004247:DNA; D013095:Spermidine