title: Identification of intrahepatic lymphocyte subpopulations in patients with fulminant hepatitis by the immunoenzymatic technique, using monoclonal antibodies.
abstract: Intrahepatic lymphocyte subpopulations were studied in 11 patients with fulminant hepatitis (FH) (of whom 9 cases died) by the immunoenzymatic technique, using monoclonal T cell specific antibodies and other reagents. In peripheral blood, the OKT4 positive cells: OKT8 positive cells ratio in FH was higher than that in normal controls. In liver tissue, OKT8 positive cells were the predominant type of T cells, and these cells were in broad contact with the surface of the hepatocyte. Leu7 positive, OKT4/4a positive and Leu 15 positive cells were scarce and did not contact the hepatocyte. These results suggest that cytotoxic T cells may play an important role in hepatocyte necrosis in patients with FH.
authors.affiliation: Third Department of Internal Medicine, Ehime University School of Medicine, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Onji
authors.forename: I
authors.initials: I
authors.lastname: Kumon
authors.forename: M
authors.initials: M
authors.lastname: Kanaoka
authors.forename: N
authors.initials: N
authors.lastname: Horiike
authors.forename: Y
authors.initials: Y
authors.lastname: Ohta
mesh_terms: D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000276:immunology; D005260:Female; D006525:Hepatitis, Viral, Human / Q000097:blood / Q000473:pathology*; D006801:Humans; D007124:Immunoenzyme Techniques; D008099:Liver / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D013601:T-Lymphocytes / Q000145:classification*
chemical_list: D000911:Antibodies, Monoclonal; D000954:Antigens, Surface