title: The construction and operation of a simple inexpensive slam freezing device for electron microscopy.
abstract: A simple and inexpensive slam freezing device has been constructed that allows consistently good rapid freezing of uncryoprotected tissue. The device employs a dry liquid-nitrogen-cooled cooper surface and a slamming plunger designed to provide rapid and uniform tissue to metal contact.
authors.forename: D P
authors.initials: DP
authors.lastname: Allison
authors.forename: C S
authors.initials: CS
authors.lastname: Daw
authors.forename: M C
authors.initials: MC
authors.lastname: Rorvik
mesh_terms: D000818:Animals; D003300:Copper; D005615:Freezing; D006652:Histological Techniques / Q000295:instrumentation*; D051379:Mice; D008854:Microscopy, Electron / Q000295:instrumentation / Q000379:methods; D009206:Myocardium / Q000648:ultrastructure*
chemical_list: D003300:Copper