A modified mouse peripheral blood lymphocyte culture system for cytogenetic analysis.
A detailed methodology is presented for culturing mouse peripheral blood lymphocytes isolated on density gradients and stimulated to divide using either phytohemagglutinin, concanavalin A, or lipopolysaccharide. The techniques described yield more than sufficient numbers of mitotic cells for analyzing sister chromatid exchange, chromosome aberrations, and micronuclei following in vitro or in vivo exposure to chemicals or radiation.
Erexson
G L
GL
Environmental Health Research and Testing, Inc., Genetic Toxicology Division, Research Triangle Park, North Carolina 27709.
Kligerman
A D
AD
D000818:Animals; D002453:Cell Cycle; D002467:Cell Nucleus / Q000648:ultrastructure; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D003584:Cytological Techniques; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects / Q000528:radiation effects / Q000648:ultrastructure*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL / Q000097:blood / Q000235:genetics; D008934:Mitogens / Q000494:pharmacology; D009152:Mutagenicity Tests / Q000379:methods; D012854:Sister Chromatid Exchange
D008934:Mitogens