Performance of the two-site immunoradiometric assay for serum thyroid-stimulating hormone. Effects of changes in solid-phase matrix and antibody coupling chemistry.
Ten solid-phases were evaluated for their usefulness in a two-site immunoradiometric assay for serum thyroid-stimulating hormone. The criteria used to assess each reagent included the minimum detection limit attainable, the change of binding over the concentration range (signal: noise ratio), and ease of preparation of the reagents. Of all the solid phases tested, Sepharose CL-4B and activated CH-Sepharose 4B showed the characteristics best suited to IRMA, while epoxy-activated Sepharose 6B and Ultrogel ACA-44 were marginally less effective.
McConway
M G
MG
Department of Pathological Biochemistry, Royal Infirmary, Glasgow, U.K.
Biggart
E M
EM
Chapman
R S
RS
D000911:Antibodies, Monoclonal; D006801:Humans; D007120:Immunochemistry; D007163:Immunosorbent Techniques; D011863:Radioimmunoassay / Q000379:methods*; D013972:Thyrotropin / Q000032:analysis*
D000911:Antibodies, Monoclonal; D013972:Thyrotropin