title: [Use of monoclonal antibodies for purifying the regulatory subunit of cAMP-dependent protein kinase].
abstract: Monoclonal antibodies against regulatory subunit of cAMP-dependent protein kinase, type II, were obtained from pig brain (R II). The immune-affinity sorbent has been synthesized on the basis of monoclonal antibodies against R II. The method was proposed for the purification of homogeneous R II with high cAMP-binding activity using immune-affinity sorbent.
authors.forename: I R
authors.initials: IR
authors.lastname: Eshba
authors.forename: N A
authors.initials: NA
authors.lastname: Kochanova
authors.forename: K M
authors.initials: KM
authors.lastname: Popov
authors.forename: T V
authors.initials: TV
authors.lastname: Bulargina
authors.forename: E S
authors.initials: ES
authors.lastname: Severin
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification; D002352:Carrier Proteins / Q000276:immunology / Q000302:isolation & purification*; D006825:Hybridomas / Q000276:immunology; D007114:Immunization / Q000379:methods; D007124:Immunoenzyme Techniques; D007163:Immunosorbent Techniques; D047908:Intracellular Signaling Peptides and Proteins; D051379:Mice; D008807:Mice, Inbred BALB C; D011494:Protein Kinases / Q000276:immunology / Q000302:isolation & purification*; D013552:Swine; D013997:Time Factors
chemical_list: D000911:Antibodies, Monoclonal; D002352:Carrier Proteins; D047908:Intracellular Signaling Peptides and Proteins; C015482:protein kinase modulator; D011494:Protein Kinases
vernacular_title: Ispol'zovanie monoklonal'nykh antitel dlia ochistki reguliatornoĭ sub''edinitsy tsAMF-zavisimoĭ proteinkinazy.