[Implantation of a mutant of Escherichia coli requiring diaminopimelic acid in the digestive tract of gnotobiotic mice].
A DAP- auxotroph mutant of Escherichia coli DP50 requiring DAP and thymidine for growth was used as the receptor strain in genetic engineering. It failed to be implanted in axenic mice. However, when an inoculum containing more than 10(7) bacteria/ml was used, the DAP+ reverse mutant devoid of requirement for DAP became implanted. When axenic mice were previously associated with Clostridium difficile containing DAP in the cell wall, the strain DAP- became implanted even when the inoculum was too small to permit implantation in axenic mice. Conversely, C. butyricum and C. perenne, whose cell walls also contain DAP, did not allow the establishment of a DAP- mutant. In animals associated with complex human flora without enterobacteria, neither of the 2 DAP- and DAP+ mutants became implanted.
Ducluzeau
R
R
Laboratoire d'Ecologie microbienne, INRA, CNRZ, Jouy en Josas, France.
Ladiré
M
M
Raibaud
P
P
D000599:Amino Acids, Diamino / Q000235:genetics*; D000818:Animals; D001422:Bacterial Adhesion; D003013:Clostridium / Q000235:genetics; D003960:Diaminopimelic Acid / Q000235:genetics*; D004064:Digestive System / Q000382:microbiology*; D004926:Escherichia coli / Q000235:genetics*; D005818:Genetic Engineering; D005856:Germ-Free Life; D051379:Mice; D008809:Mice, Inbred C3H; D009154:Mutation
D000599:Amino Acids, Diamino; D003960:Diaminopimelic Acid
Implantation d'un mutant de Escherichia coli exigeant en acide diaminopimélique dans le tube digestif de souris gnotoxéniques.