Novel non-isotopic in situ hybridization technique detects small (1 Kb) unique sequences in routinely G-banded human chromosomes: fine mapping of N-myc and beta-NGF genes.
A novel in situ hybridization technique is described. This non-radioactive technique combines, for the first time, the high spacial resolution and rapid signal development of the non-isotopic approach with the previously unrivalled sensitivity of autoradiography. The procedure, which employs biotin labelled DNA probes and a streptavidin-alkaline phosphatase based detection system, is compatible with pre G-banding and can be performed on archival material. Unique sequences as small as 1 Kb are detectable. Using this technique, we have mapped the N-myc oncogene and the gene for beta-Nerve Growth Factor to 2p24 and 1p13 respectively.
Garson
J A
JA
van den Berghe
J A
JA
Kemshead
J T
JT
D000469:Alkaline Phosphatase; D001399:Azure Stains; D001426:Bacterial Proteins; D001483:Base Sequence; D001710:Biotin; D002871:Chromosome Banding / Q000379:methods; D002874:Chromosome Mapping / Q000379:methods*; D004274:DNA, Recombinant; D005796:Genes; D006801:Humans; D009414:Nerve Growth Factors / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D009857:Oncogenes; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D016271:Proto-Oncogene Proteins c-myc; D019809:Streptavidin
D001399:Azure Stains; D001426:Bacterial Proteins; D004274:DNA, Recombinant; D009414:Nerve Growth Factors; D011518:Proto-Oncogene Proteins; D016271:Proto-Oncogene Proteins c-myc; D001710:Biotin; D019809:Streptavidin; D000469:Alkaline Phosphatase