Deconjugation of bile acids by human intestinal bacteria implanted in germ-free rats.
Fecal bile acids in germ-free rats were analyzed after inoculation with Bacteroides vulgatus, Bifidobacterium longum, Escherichia coli or Clostridium ramosum. B. vulgatus preferentially deconjugated tauro-beta-muricholic acid and B. longum taurocholic acid. C. ramosum deconjugated both bile acids, but E. coli deconjugated neither. 7 alpha-Dehydroxylation of bile acids was negligible even after 18 days of inoculation, but a small amount of 7-oxo-bile acids, less than 5%, was formed. Fecal excretion of bile acids increased after inoculation with B. vulgatus, B. longum and C. ramosum, but not with E. coli.
Chikai
T
T
Shionogi Research Laboratories, Osaka, Japan.
Nakao
H
H
Uchida
K
K
D000818:Animals; D001439:Bacteroides / Q000378:metabolism*; D001644:Bifidobacterium / Q000378:metabolism*; D001647:Bile Acids and Salts / Q000378:metabolism*; D002635:Chenodeoxycholic Acid / Q000378:metabolism; D002793:Cholic Acids / Q000378:metabolism; D003013:Clostridium / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism*; D005243:Feces / Q000382:microbiology; D005856:Germ-Free Life; D006801:Humans; D007422:Intestines / Q000382:microbiology*; D008297:Male; D051381:Rats
D001647:Bile Acids and Salts; D002793:Cholic Acids; D002635:Chenodeoxycholic Acid