A colorimetric liquid culture assay of a growth factor for primitive murine macrophage progenitor cells.
Synergistic factors from media conditioned (CM) by human placentas or the 5637 human bladder carcinoma cell line (SFH-HPCM and SFH-5637 respectively) have the ability to stimulate early progenitor cells in mouse bone marrow to form large colonies in agar cultures after 12-14 days, in the presence of CSF-1. Culture conditions have been examined and a quicker and more convenient liquid culture assay has been developed for this factor, using a tetrazolium salt to quantitate cell proliferation. The use of flat-bottomed vessels, high cell density, supra-optimal doses of CSF-1 or the addition of WEHI-3-CM to these cultures, all resulted in a decrease in the required incubation time. In combination, these modifications reduced the assay time to 4 days.
Kriegler
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AB
Cell Biology Group, Peter MacCallum Cancer Institute, Melbourne, Victoria, Australia.
Bradley
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TR
Hodgson
G S
GS
McNiece
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IK
D000818:Animals; D002277:Carcinoma / Q000032:analysis; D002454:Cell Differentiation / Q000187:drug effects; D003114:Colony-Forming Units Assay / Q000379:methods*; D003115:Colony-Stimulating Factors / Q000032:analysis* / Q000494:pharmacology; D003124:Colorimetry; D005260:Female; D006133:Growth Substances / Q000032:analysis* / Q000494:pharmacology; D016298:Hematopoietic Cell Growth Factors; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D007951:Leukemia, Myeloid / Q000378:metabolism; D008264:Macrophages / Q000166:cytology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D010920:Placenta / Q000032:analysis; D014407:Tumor Cells, Cultured / Q000032:analysis; D001749:Urinary Bladder Neoplasms / Q000032:analysis
D003115:Colony-Stimulating Factors; D006133:Growth Substances; D016298:Hematopoietic Cell Growth Factors