title: Studies on induced Klebsiella mastitis with relationships among N-acetyl-beta-D-glucosaminidase, bacterial and somatic cell counts.
abstract: Mastitis was induced by infusion of 3,000-7,000 colony-forming units of Klebsiella pneumoniae into the left rear quarter of four primiparous Holstein-Freisians. Greater than 100 organisms were recovered from a quarter milk sample within 2 hr and in 87.5% of the hourly cultures taken after 8 hr. Bacteria, California Mastitis Tests, somatic cell counts and N-acetyl-beta-D-glucosaminidase levels differed significantly from that of control quarters in infused cows after hr 6, 8, 9 and 9 respectively. High correlations were obtained between somatic cell count and N-acetyl-beta-D-glucosaminidase (r = .97) and N-acetyl-beta-D-glucosaminidase and natural log bacterial count (r. = .88). N-acetyl-beta-D-glucosaminidase was related to natural log bacterial counts of samples taken 1, 2, 3, and 4 hr prior to the test (rN-acetyl-beta-D-glucosaminidase and natural log bacterial count (r = .91, .92, .93, and .95).
authors.affiliation: Department of Animal Science, University of Vermont, Burlington 05405.
authors.forename: J R
authors.initials: JR
authors.lastname: Kunkel
authors.forename: R B
authors.initials: RB
authors.lastname: Bushnell
authors.forename: J
authors.initials: J
authors.lastname: Cullor
authors.forename: J
authors.initials: J
authors.lastname: Aleong
mesh_terms: D000118:Acetylglucosaminidase / Q000032:analysis*; D000818:Animals; D002417:Cattle; D002452:Cell Count; D005260:Female; D006596:Hexosaminidases / Q000032:analysis*; D007710:Klebsiella Infections / Q000201:enzymology / Q000473:pathology / Q000662:veterinary*; D007711:Klebsiella pneumoniae; D008414:Mastitis, Bovine / Q000201:enzymology / Q000382:microbiology* / Q000473:pathology; D008892:Milk / Q000032:analysis* / Q000166:cytology / Q000201:enzymology / Q000382:microbiology
chemical_list: D006596:Hexosaminidases; D000118:Acetylglucosaminidase