title: The osmotically regulated proU locus of Salmonella typhimurium encodes a periplasmic betaine-binding protein.
abstract: The proU locus of Salmonella typhimurium encodes an osmotically induced betaine transport system. We have identified a 31 kDa periplasmic protein, encoded by proU, whose synthesis is induced by osmotic stress. A specific betaine-binding activity with a KD of about 1 microM is also present in the periplasm of osmotically induced cells. This activity is absent in those proU mutants which lack the 31 kDa periplasmic protein. Thus, ProU is a periplasmic binding-protein-dependent transport system.
authors.affiliation: Department of Biochemistry, University of Dundee, UK.
authors.forename: C F
authors.initials: CF
authors.lastname: Higgins
authors.forename: L
authors.initials: L
authors.lastname: Sutherland
authors.forename: J
authors.initials: J
authors.lastname: Cairney
authors.forename: I R
authors.initials: IR
authors.lastname: Booth
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001622:Betaine / Q000235:genetics / Q000378:metabolism*; D001692:Biological Transport; D002874:Chromosome Mapping; D012486:Salmonella typhimurium / Q000235:genetics* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D001622:Betaine