Establishment of the steady state in glucose-limited chemostat cultures of Klebsiella pneumoniae.
To investigate the relationship between growth rate and concentration of the nutrient that limits growth, 'Klebsiella aerogenes' NCTC 418 (K. pneumoniae) was grown in a glucose-limited chemostat. The actual time required to establish a steady-state glucose concentration exceeded that expected theoretically. Apparently, there is a long-term adaptation of the cells to nutrient limitation. As yet, it is not clear whether this has a phenotypic or genetic origin. In the final steady state, the dependence of the growth rate on glucose concentration could be mathematically described equally well by a hyperbolic and by a logarithmic function.
Rutgers
M
M
Laboratory of Biochemistry, University of Amsterdam, The Netherlands.
Teixeira de Mattos
M J
MJ
Postma
P W
PW
Van Dam
K
K
D003470:Culture Media; D005947:Glucose / Q000378:metabolism*; D007700:Kinetics; D007711:Klebsiella pneumoniae / Q000254:growth & development*
D003470:Culture Media; D005947:Glucose