Localization of glycoproteins in insulin secretory granules by ultrastructural autoradiography.
To determine whether or not the secretory granules of insulin-secreting cells contained glycoproteins, isolated rat pancreatic islets were incubated for 2 and 4 hr in a medium containing L-[3H]-fucose. Quantitative analysis of high-resolution electron microscopic autoradiographs of the insulin-secreting beta cells demonstrated that glycoproteins with fucose residues are contained within the insulin secretory granule.
Haddad
A
A
Kopriwa
B
B
Pelletier
G
G
D000818:Animals; D001345:Autoradiography; D003594:Cytoplasmic Granules / Q000648:ultrastructure*; D005643:Fucose / Q000378:metabolism; D006023:Glycoproteins / Q000032:analysis*; D007328:Insulin; D007515:Islets of Langerhans / Q000648:ultrastructure*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D006023:Glycoproteins; D007328:Insulin; D005643:Fucose