title: H1 histone synthesis and phosphorylation in mouse mammary gland in vitro.
abstract: The synthesis and phosphorylation of H1 histones were studied in organ cultures of midpregnant mouse mammary glands exposed to various combinations of insulin, cortisol and prolactin over a 48-h period. The synthesis of specific H1 histone subtypes was changed only when all three hormones were present, and the effect was most pronounced during the first 24 h of culture, a period of cell replication. The 3-hormone combination also stimulated the phosphorylation of the N-terminal region of the H1 histone, and this also occurred maximally during the first 24 h of culture. The enhanced phosphorylation of the N-terminal region of the H1 histone included a site sensitive to phosphorylation by cyclic AMP-dependent protein kinase. Thus, hormones which stimulate mammary development in vitro influence the synthesis and specific phosphorylation of H1 histones during a period of cell replication preceding the expression of milk protein genes.
authors.affiliation: Department of Experimental Biology, Roswell Park Memorial Institute, Buffalo, NY 14263.
authors.forename: P
authors.initials: P
authors.lastname: Hohmann
authors.forename: C
authors.initials: C
authors.lastname: Hughes
mesh_terms: D000818:Animals; D005260:Female; D006657:Histones / Q000096:biosynthesis*; D006854:Hydrocortisone / Q000494:pharmacology*; D007328:Insulin / Q000494:pharmacology*; D008321:Mammary Glands, Animal / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D009924:Organ Culture Techniques; D010449:Peptide Mapping; D010766:Phosphorylation; D011247:Pregnancy; D011388:Prolactin / Q000494:pharmacology*
chemical_list: D006657:Histones; D007328:Insulin; D011388:Prolactin; D006854:Hydrocortisone