title: Mixed blood chimerism in T cell-depleted bone marrow transplant recipients: evaluation using DNA polymorphisms.
abstract: We have used DNA sequence polymorphism analysis to document engraftment after T cell-depleted bone marrow transplantation (BMT), with a selected panel of four DNA probes. In contrast to nondepleted BMT recipients, the patients who received T cell-depleted marrow exhibited a mixed blood chimerism. This mosaicism was observed before graft failure or relapse in six patients. However, in five other patients, this mixed chimerism was not followed by these complications with a follow-up of 9 to 31 months after transplantation. Our results support the hypothesis that transplanted bone marrow T cells may help to maintain engraftment by eliminating host cells that can cause graft failure.
authors.affiliation: INSERM U.91, Department of Genetics, Creteil, France.
authors.forename: S
authors.initials: S
authors.lastname: Bretagne
authors.forename: M
authors.initials: M
authors.lastname: Vidaud
authors.forename: M
authors.initials: M
authors.lastname: Kuentz
authors.forename: C
authors.initials: C
authors.lastname: Cordonnier
authors.forename: T
authors.initials: T
authors.lastname: Henni
authors.forename: G
authors.initials: G
authors.lastname: Vinci
authors.forename: M
authors.initials: M
authors.lastname: Goossens
authors.forename: J P
authors.initials: JP
authors.lastname: Vernant
mesh_terms: D000328:Adult; D001402:B-Lymphocytes / Q000166:cytology; D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation; D002678:Chimera; D004273:DNA, Neoplasm / Q000235:genetics*; D005260:Female; D006098:Granulocytes / Q000166:cytology; D006801:Humans; D007938:Leukemia / Q000097:blood / Q000235:genetics / Q000628:therapy*; D008297:Male; D009693:Nucleic Acid Hybridization; D011110:Polymorphism, Genetic; D013601:T-Lymphocytes / Q000166:cytology*
chemical_list: D004273:DNA, Neoplasm