title: Photolabelling of a mitochondrially encoded subunit of NADH dehydrogenase with [3H]dihydrorotenone.
abstract: Mitochondrial NADH dehydrogenase from bovine heart was photolabelled with the inhibitor [3H]dihydrorotenone. A constituent of the hydrophobic domain of the enzyme of Mr 33,000 was the major site of labelling. The identity of this protein with the mitochondrially encoded ND-1 gene product was established by immunoblotting and immunoprecipitation with an antiserum raised to the expected C-terminal sequence of the human ND-1 gene product.
authors.forename: F G
authors.initials: FG
authors.lastname: Earley
authors.forename: S D
authors.initials: SD
authors.lastname: Patel
authors.forename: I
authors.initials: I
authors.lastname: Ragan
authors.forename: G
authors.initials: G
authors.lastname: Attardi
mesh_terms: D000818:Animals; D002417:Cattle; D003579:Cytochrome Reductases / Q000378:metabolism*; D006801:Humans; D007120:Immunochemistry; D007158:Immunologic Techniques; D008929:Mitochondria, Heart / Q000201:enzymology*; D009245:NADH Dehydrogenase / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D010777:Photochemistry; D011485:Protein Binding; D012402:Rotenone / Q000031:analogs & derivatives* / Q000378:metabolism
chemical_list: D012402:Rotenone; C044985:1',2'-dihydrorotenone; D003579:Cytochrome Reductases; D009245:NADH Dehydrogenase