Identification and analysis of nine metabolites of cyclosporine in whole blood by liquid chromatography. 2: Comparison of patients' results.
We used a "high-performance" liquid-chromatographic assay [for parent cyclosporine (CsA) and nine metabolites] and a radioimmunoassay to detail the diversity of results among whole-blood samples from patients with transplanted organs. Heterogeneous populations of metabolites in samples collected just before the next dose of CsA were detected by HPLC, with CsA, M17, M1, or M8 predominating; M21, M203-218, MUNDF1, and M18 were detected in lesser amounts. Results by HPLC vs RIA for CsA or for individual metabolites vs CsA (or RIA) were diverse, with correlation coefficients (r) ranging from 0.058 to 0.933. RIA vs HPLC(sum of CsA + metabolites) gave the best comparison (slope = 0.931, y-intercept 14 micrograms/L, r = 0.933); but the scatter of data about the regression line remained significant (Sy/x = 132 micrograms/L). Most important, RIA/HPLC(CsA) vs HPLC(sum of metabolites) was remarkably poor (r = 0.222). A 12-h pharmacokinetic curve (for drug concentrations in a heart-transplant patient) displayed dissimilar times for peak concentrations of CsA and metabolites; each differed in the proportion (48% to 81% of peak concentration) eliminated from blood over the 12 h. These studies exemplify the utility of a more-inclusive, specific assay to monitor the diverse disposition of cyclosporines in patients and to demonstrate the errors associated with use of the RIA/HPLC ratio technique to predict metabolite concentrations.
Lensmeyer
G L
GL
Department of Pathology and Laboratory Medicine, University of Wisconsin, Madison 53792.
Wiebe
D A
DA
Carlson
I H
IH
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003524:Cyclosporins / Q000097:blood* / Q000493:pharmacokinetics; D016027:Heart Transplantation; D006801:Humans; D007165:Immunosuppression Therapy; D016031:Liver Transplantation; D011863:Radioimmunoassay
D003524:Cyclosporins