Expression of human adenosine deaminase in nonhuman primates after retrovirus-mediated gene transfer.
Primate bone marrow cells were infected with a retroviral vector carrying the genes for human adenosine deaminase (h-ADA) and bacterial neomycin resistance (neor). The infected cells were infused back into the lethally irradiated donor animals. Several monkeys fully reconstituted and were shown to express the h-ADA and neor genes at low levels in their recirculating hematopoietic cells for short periods of time.
Kantoff
P W
PW
Gillio
A P
AP
McLachlin
J R
JR
Bordignon
C
C
Eglitis
M A
MA
Kernan
N A
NA
Moen
R C
RC
Kohn
D B
DB
Yu
S F
SF
Karson
E
E
D000243:Adenosine Deaminase / Q000235:genetics*; D000818:Animals; D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation; D004247:DNA / Q000235:genetics; D004351:Drug Resistance / Q000235:genetics; D006412:Hematopoietic Stem Cells / Q000201:enzymology; D006801:Humans; D008214:Lymphocytes / Q000201:enzymology; D008252:Macaca fascicularis; D009000:Monocytes / Q000201:enzymology; D009355:Neomycin; D009693:Nucleic Acid Hybridization; D009700:Nucleoside Deaminases / Q000235:genetics*; D012190:Retroviridae / Q000235:genetics*; D014170:Transformation, Genetic; D014916:Whole-Body Irradiation
D004247:DNA; D009700:Nucleoside Deaminases; D000243:Adenosine Deaminase; D009355:Neomycin