[Use of monoclonal antibodies for purifying the regulatory subunit of cAMP-dependent protein kinase].
Monoclonal antibodies against regulatory subunit of cAMP-dependent protein kinase, type II, were obtained from pig brain (R II). The immune-affinity sorbent has been synthesized on the basis of monoclonal antibodies against R II. The method was proposed for the purification of homogeneous R II with high cAMP-binding activity using immune-affinity sorbent.
Eshba
I R
IR
Kochanova
N A
NA
Popov
K M
KM
Bulargina
T V
TV
Severin
E S
ES
D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification; D002352:Carrier Proteins / Q000276:immunology / Q000302:isolation & purification*; D006825:Hybridomas / Q000276:immunology; D007114:Immunization / Q000379:methods; D007124:Immunoenzyme Techniques; D007163:Immunosorbent Techniques; D047908:Intracellular Signaling Peptides and Proteins; D051379:Mice; D008807:Mice, Inbred BALB C; D011494:Protein Kinases / Q000276:immunology / Q000302:isolation & purification*; D013552:Swine; D013997:Time Factors
D000911:Antibodies, Monoclonal; D002352:Carrier Proteins; D047908:Intracellular Signaling Peptides and Proteins; C015482:protein kinase modulator; D011494:Protein Kinases
Ispol'zovanie monoklonal'nykh antitel dlia ochistki reguliatornoĭ sub''edinitsy tsAMF-zavisimoĭ proteinkinazy.