The role of mononuclear cell products as modulators of cartilage degradation.
Human articular cartilage cultured in the presence of interleukin 1 (IL-1) produced from activated peripheral blood mononuclear cells, exhibits the concomitant release of a latent metalloproteinase and fragments of proteoglycan. The manner in which the activated form of the metalloproteinase degrades proteoglycan subunit and link protein suggests a role for this enzyme in cartilage degradation in vivo. While cartilage cultured in the continuous presence of purified IL-1 shows sustained release of metalloproteinase, tissue cultured in the presence of the total products released by activated mononuclear cells from some individuals showed initial stimulation followed by a decline, suggesting the presence of a modulator of IL-1 action.
Mort
J S
JS
Campbell
I K
IK
Guttman
A
A
Golds
E E
EE
Roughley
P J
PJ
D002358:Cartilage, Articular / Q000187:drug effects / Q000378:metabolism*; D046508:Culture Techniques; D010450:Endopeptidases / Q000378:metabolism; D006801:Humans; D007375:Interleukin-1 / Q000502:physiology*; D008666:Metalloendopeptidases; D011509:Proteoglycans / Q000378:metabolism
D007375:Interleukin-1; D011509:Proteoglycans; D010450:Endopeptidases; D008666:Metalloendopeptidases