[Effectiveness of the promoter Ptac' in vitro and in mini-cells].
The efficiency of hybrid promotor Ptac', comprising a synthetic trp-promoter and lacUV5 "-10" sequence, was studied. By means of electrophoresis and hybridization of RNA-products obtained in vitro and in minicells, with promotor-containing plasmids; the hybrid promotor was found to be 6 times and 3-4 times as efficient as trp and lacUV5 promoters, respectively.
Denisova
L Ia
LIa
Zagrebel'nyĭ
S N
SN
Zakabunin
A I
AI
Kileva
E V
EV
Kovzhov
V A
VA
D004926:Escherichia coli / Q000235:genetics; D005818:Genetic Engineering; D007763:Lac Operon; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D011401:Promoter Regions, Genetic; D014364:Tryptophan / Q000235:genetics
D014364:Tryptophan
Izuchenie éffektivnosti promotora Ptac' in vitro i v mini-kletkakh.