title: GnRH secretion throughout the ovine estrous cycle.
abstract: In order to define the patterns of gonadotropin-releasing hormone (GnRH) secretion during the estrous cycle of the sheep we sampled hypophysial portal blood from conscious animals on day 1 of the cycle (n = 1), during the luteal phase (n = 8), during the follicular phase (n = 6) and during the preovulatory luteinizing hormone (LH) surge (n = 6). At the same time, we sampled jugular blood to measure plasma LH concentrations. During day 1 we noted regular GnRH pulses, whereas GnRH pulse amplitude and frequency were more variable in the luteal phase of the cycle. In the transition from the luteal phase to the follicular phase the GnRH pulse frequency increased and the amplitude decreased. Around the time of the LH surge we noted 3 types of secretory profiles for GnRH. In one sheep (type 1) there was a large GnRH pulse at the onset of the LH surge followed by very little activity during the surge. In two sheep (type 2) the GnRH profile did not change between the late follicular phase and the onset of the LH surge. In the remaining three sheep (type 3) there was a clear increase in the secretion of GnRH at the onset of the LH surge. With the exclusion of the type 1 sheep the GnRH pulse frequency was maximal (2 pulses/h) at the time of the LH surge; average portal GnRH levels were also maximal at this time.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.forename: I J
authors.initials: IJ
authors.lastname: Clarke
authors.forename: G B
authors.initials: GB
authors.lastname: Thomas
authors.forename: B
authors.initials: B
authors.lastname: Yao
authors.forename: J T
authors.initials: JT
authors.lastname: Cummins
mesh_terms: D000818:Animals; D004971:Estrus / Q000378:metabolism*; D005260:Female; D005498:Follicular Phase; D007986:Luteinizing Hormone / Q000097:blood; D010902:Pituitary Gland / Q000378:metabolism*; D010906:Pituitary Hormone-Releasing Hormones / Q000097:blood / Q000378:metabolism*; D012756:Sheep / Q000502:physiology
chemical_list: D010906:Pituitary Hormone-Releasing Hormones; D007986:Luteinizing Hormone