title: Cross-reaction of a plant protein kinase with antiserum raised against a sequence from bovine brain protein kinase C regulatory sub-unit.
abstract: The partly purified calcium-dependent, phospholipid-activated protein kinase from A. tricolor seedlings contains three major protein species (Mr = 84,500, 65,000 and 40,000) which cross-react with antiserum raised against the regulatory domain of bovine brain protein kinase C. Two of these species (Mr = 84,500 and 40,000) were phosphorylated when the preparation was incubated with [gamma-32P] ATP. It is suggested that the three cross-reacting proteins correspond to native enzyme, partly proteolysed enzyme and regulatory sub-unit respectively.
authors.forename: D C
authors.initials: DC
authors.lastname: Elliott
authors.forename: Y S
authors.initials: YS
authors.lastname: Kokke
mesh_terms: D001921:Brain / Q000201:enzymology*; D003429:Cross Reactions; D007106:Immune Sera; D007124:Immunoenzyme Techniques; D046911:Macromolecular Substances; D008970:Molecular Weight; D010944:Plants / Q000201:enzymology*; D011493:Protein Kinase C / Q000276:immunology*
chemical_list: D007106:Immune Sera; D046911:Macromolecular Substances; D011493:Protein Kinase C