Automatic nitrous oxide synchronization of mitotic human cell cultures.
Large numbers of human cells can be reversibly arrested in mitosis by high-pressure nitrous oxide. The optimum schedule for this arrest requires that the high-pressure block be started around midnight to provide a mitotic population that can be released the next morning to progress in synchrony through the next cell cycle. We describe a simple and safe device which can be set up at the end of the day and automatically exposes the cells to high-pressure nitrous oxide overnight.
Downes
C S
CS
University of Cambridge, Department of Zoology, United Kingdom.
Unwin
D M
DM
Northfield
R G
RG
Berry
M J
MJ
D002478:Cells, Cultured; D003584:Cytological Techniques / Q000295:instrumentation; D006801:Humans; D008938:Mitosis / Q000187:drug effects*; D009609:Nitrous Oxide
D009609:Nitrous Oxide