title: 2-Phenylethylamine catabolism by Escherichia coli K12.
abstract: Escherichia coli K12 grows on 2-phenylethylamine as sole carbon and energy source by converting it, via phenylacetaldehyde, to phenylacetic acid. Phenylacetaldehyde was formed by the action of an inducible amine oxidase and catalase activity was increased sixfold, presumably to ensure removal of the H2O2 that was expected to be a product of the amine oxidation. The phenylacetaldehyde was oxidized to phenylacetic acid by an inducible NAD+-dependent dehydrogenase. Mutants defective in phenylacetaldehyde dehydrogenase cannot grow on 2-phenylethylamine as carbon and energy source but can still use it as a nitrogen source.
authors.affiliation: Department of Biochemistry, University of Leicester, UK.
authors.forename: S
authors.initials: S
authors.lastname: Parrott
authors.forename: S
authors.initials: S
authors.lastname: Jones
authors.forename: R A
authors.initials: RA
authors.lastname: Cooper
mesh_terms: D000445:Aldehyde Oxidoreductases / Q000378:metabolism; D006631:Amine Oxidase (Copper-Containing); D002374:Catalase / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology / Q000378:metabolism*; D029968:Escherichia coli Proteins; D009154:Mutation; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000378:metabolism; D010627:Phenethylamines / Q000378:metabolism*
chemical_list: D029968:Escherichia coli Proteins; D010627:Phenethylamines; D002374:Catalase; D000445:Aldehyde Oxidoreductases; C053855:phenylacetaldehyde dehydrogenase; D006631:Amine Oxidase (Copper-Containing); D000587:Oxidoreductases Acting on CH-NH Group Donors