title: Autographa californica nuclear polyhedrosis virus efficiently enters but does not replicate in poikilothermic vertebrate cells.
abstract: The host range of the insect virus Autographa californica nuclear polyhedrosis virus (AcMNPV) was examined. AcMNPV could not initiate a productive infection in frog, turtle, trout, or codling moth cell lines. After exposure to AcMNPV, neither viral DNA nor RNA synthesis could be detected in these cell lines as assayed by nucleic acid probe hybridization. Entry of AcMNPV nucleocapsids into the cytoplasm and viral DNA into the nucleus, however, was as efficient in the nonpermissive cell lines as it was in a permissive insect cell line. The data suggest that the block in AcMNPV infection in these nonpermissive cell lines is at a stage subsequent to viral DNA entry into the nucleus.
authors.forename: J
authors.initials: J
authors.lastname: Brusca
authors.forename: M
authors.initials: M
authors.lastname: Summers
authors.forename: J
authors.initials: J
authors.lastname: Couch
authors.forename: L
authors.initials: L
authors.lastname: Courtney
mesh_terms: D000818:Animals; D002213:Capsid / Q000502:physiology; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D004279:DNA, Viral / Q000378:metabolism; D007304:Insect Viruses / Q000502:physiology*; D008854:Microscopy, Electron; D013045:Species Specificity; D014337:Trout / Q000382:microbiology; D014426:Turtles / Q000382:microbiology; D014758:Viral Core Proteins / Q000502:physiology; D014779:Virus Replication; D014982:Xenopus laevis / Q000382:microbiology
chemical_list: D004279:DNA, Viral; D014758:Viral Core Proteins