Degradation of the framework of the Chlamydomonas cell wall by proteases present in a commercially available alpha-amylase preparation.
A commercially available alpha-amylase derived from Bacillus licheniformis contained an enzymatic activity able to degrade the inner portion or framework of the cell wall of Chlamydomonas reinhardtii. Both the wall-degrading activity and the contaminating protease were destroyed by heating the alpha-amylase preparation at 90 degrees C for 30 min. Since the alpha-amylase activity was uneffected by heat treatment, we conclude that it was not the alpha-amylase but the contaminating protease in the preparation that was responsible for the cell wall-degrading activity.
Imam
S H
SH
Department of Cell Biology, University of Texas Health Science Center, Dallas 75235.
Snell
W J
WJ
D001407:Bacillus / Q000201:enzymology; D002473:Cell Wall / Q000378:metabolism; D002696:Chlamydomonas / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D006358:Hot Temperature; D010447:Peptide Hydrolases / Q000378:metabolism*; D000516:alpha-Amylases / Q000378:metabolism*
D000516:alpha-Amylases; D010447:Peptide Hydrolases