Flow cytometric analysis of relative mean DNA content of urogenital cancer cells in fresh and paraffin-embedded materials.
The relative mean DNA content calculation was performed by flow cytometry on single cell suspensions prepared from fresh and paraffin-embedded specimens of 10 patients with surgically resected urogenital cancer. Samples were processed by a modified method of Hedley et al. including two hours of pepsinizing time, ribonuclease digestion, and propidium iodide staining. The mean DNA content which is a quantitative description of flow cytometric characteristics was significantly correlated between the fresh and paraffin-embedded materials (n = 10, r = 0.869, p less than 0.01). This method allows for the objective, retrospective analysis of DNA content in relation to diagnosis and prognosis of urogenital cancer.
Nakamura
K
K
Department of Urology, New York Medical College, Valhalla.
Simon
A L
AL
Kasabian
N G
NG
Addonizio
J C
JC
Choudhury
M
M
Nagamatsu
G R
GR
Rossi
J A
JA
Chiao
J W
JW
D004273:DNA, Neoplasm / Q000032:analysis*; D005404:Fixatives; D005434:Flow Cytometry; D005557:Formaldehyde; D006652:Histological Techniques; D006801:Humans; D007680:Kidney Neoplasms / Q000032:analysis; D008297:Male; D010232:Paraffin; D011471:Prostatic Neoplasms / Q000032:analysis; D001749:Urinary Bladder Neoplasms / Q000032:analysis; D014565:Urogenital Neoplasms / Q000032:analysis*
D004273:DNA, Neoplasm; D005404:Fixatives; D005557:Formaldehyde; D010232:Paraffin