title: Improved sensitivity in assays for binding of novel beta-lactam antibiotics to penicillin-binding proteins of Escherichia coli.
abstract: Tigemonam and temocillin, but not aztreonam, bound to penicillin-binding proteins (PBPs) 1a and 3 of Escherichia coli with apparent improved affinity when challenged with benzylpenicillin at lowered temperatures. Half times for deacylation of the tigemonam-PBP complexes were shorter than were those of the corresponding aztreonam-PBP complexes. The implications of the routine testing of PBP affinities are discussed.
authors.forename: K
authors.initials: K
authors.lastname: Bush
authors.forename: S A
authors.initials: SA
authors.lastname: Smith
authors.forename: S
authors.initials: S
authors.lastname: Ohringer
authors.forename: S K
authors.initials: SK
authors.lastname: Tanaka
authors.forename: D P
authors.initials: DP
authors.lastname: Bonner
mesh_terms: D000900:Anti-Bacterial Agents / Q000378:metabolism*; D001426:Bacterial Proteins; D002352:Carrier Proteins / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D003640:Dealkylation; D004926:Escherichia coli / Q000378:metabolism*; D006602:Hexosyltransferases; D007700:Kinetics; D008826:Microbial Sensitivity Tests; D008997:Monobactams / Q000378:metabolism; D002267:Muramoylpentapeptide Carboxypeptidase / Q000378:metabolism*; D010400:Penicillin G / Q000378:metabolism; D046915:Penicillin-Binding Proteins; D010458:Peptidyl Transferases; D011485:Protein Binding; D013696:Temperature
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D002352:Carrier Proteins; D008997:Monobactams; D046915:Penicillin-Binding Proteins; C052020:tigemonam; D010458:Peptidyl Transferases; D006602:Hexosyltransferases; D002267:Muramoylpentapeptide Carboxypeptidase; D010400:Penicillin G