Transformation of yeast spheroplasts without cell fusion.
The efficiency of genetic transformation of Saccharomyces cerevisiae spheroplasts has been increased 10- to 100-fold over previously published procedures. Optimal transformation frequencies for single-stranded and double-stranded replicating plasmids are 2 X 10(7) and 5 X 10(6) transformants/microgram, respectively. At saturating DNA concentrations, 12 and 3%, respectively, of the viable spheroplasts contain plasmid DNA. The percentage of transformants that have undergone nuclear fusion varies from 0.1 to 3%, indicating that fusion is not required for the uptake of DNA by yeast spheroplasts.
Burgers
P M
PM
Department of Biological Chemistry, Washington University School of Medicine, St. Louis, Missouri 63110.
Percival
K J
KJ
D002459:Cell Fusion; D004271:DNA, Fungal / Q000235:genetics; D004277:DNA, Single-Stranded / Q000235:genetics; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D013104:Spheroplasts; D014170:Transformation, Genetic
D004271:DNA, Fungal; D004277:DNA, Single-Stranded