Studies on the sequence and structure of the Escherichia coli K-12 nupG gene, encoding a nucleoside-transport system.
The nupG gene, encoding one of the two active nucleoside-transport systems in Escherichia coli K-12, has been cloned on the multicopy plasmid pBR322 and derivatives thereof. The recombinant plasmids complemented a chromosomal nupG mutation. A genetic map was determined by digestion with restriction endonucleases and the nucleotide sequence of a 3-kb stretch of DNA has been determined on fragments cloned into M13 phages. An open reading frame of 1254 bp, encoding a protein with a calculated molecular mass of 45.333 kDa, was deduced to be the coding region of nupG. Minicell-forming strains carrying plasmids containing this gene were shown to produce a hydrophobic, membrane-bound polypeptide with an apparent molecular mass of approximately 43 kDa.
Westh Hansen
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Institute of Biological Chemistry B, University of Copenhagen, Denmark.
Jensen
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N
Munch-Petersen
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A
D001483:Base Sequence; D001665:Binding Sites; D001693:Biological Transport, Active; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D005796:Genes; D008969:Molecular Sequence Data; D009705:Nucleosides / Q000378:metabolism*; D010452:Peptide Biosynthesis; D010455:Peptides / Q000235:genetics; D010957:Plasmids; D011401:Promoter Regions, Genetic
D009705:Nucleosides; D010455:Peptides