Expression of a novel R-plasmid pEB017 compared to pKM101 in Escherichia coli wild-type, recA and uvrA strains.
The expression of bacterial resistance to UV irradiation and nitrofurantoin by a novel R-plasmid pEB017 in DNA-repair-proficient (wild-type) and -deficient (recA; uvrA) host strains was compared to the effects of plasmid pKM101 in the isogenic strains. pEB017 partially protected the uvrA strain, and completely protected the wild-type and recA strains from the killing effect of UV irradiation; pKM101 had no effect on the recA strain and only enhanced the survival of the wild-type and the uvrA strains after UV irradiation. pEB017 conferred nitrofurantoin resistance 10-fold on the wild-type and the recA strains and 4-fold on the uvrA strain; pKM101 did not confer nitrofurantoin resistance on the wild-type and recA strains but gave 4-fold resistance in the uvrA strain.
Obaseiki-Ebor
E E
EE
Department of Pharmaceutical Microbiology, University of Benin, Nigeria.
Akerele
J O
JO
D004249:DNA Damage; D004260:DNA Repair; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics* / Q000528:radiation effects; D005786:Gene Expression Regulation; D005798:Genes, Bacterial; D009582:Nitrofurantoin / Q000633:toxicity; D009601:Nitroreductases / Q000378:metabolism; D011815:R Factors; D011938:Rec A Recombinases / Q000502:physiology; D014466:Ultraviolet Rays
D009582:Nitrofurantoin; C029492:aromatic NADH-dependent nitroreductase; D009601:Nitroreductases; D011938:Rec A Recombinases