Unfixed material embedded in a methacrylate resin (Technovit 7100) for immunofluorescent staining.
Wheat grains were embedded with or without fixation in Technovit 7100 or in paraffin. This enabled us to produce 4 microns sections for immunofluorescent staining to see whether serum of patients with baker's asthma contained IgG antibodies against wheat grains. Embedding without fixation in Technovit 7100 appeared to be suitable for immunofluorescent staining and gave superior results to protocols requiring fixation.
Popp
W
W
Zwick
H
H
D000179:Acrylates; D000180:Acrylic Resins; D000941:Antigens / Q000276:immunology; D001249:Asthma / Q000276:immunology; D000431:Ethanol; D005404:Fixatives; D005455:Fluorescent Antibody Technique; D006801:Humans; D008689:Methacrylates; D009784:Occupational Diseases / Q000276:immunology; D010232:Paraffin; D014908:Triticum / Q000276:immunology
D000179:Acrylates; D000180:Acrylic Resins; D000941:Antigens; D005404:Fixatives; D008689:Methacrylates; D000431:Ethanol; D010232:Paraffin; C053012:Technovit 7100