Decreased insulin binding and antilipolytic response in adipocytes from patients with Cushing's syndrome.
Human adipocytes from patients with chronic endogenous hypercortisolism (Cushing's syndrome) showed a statistically significant decrease in insulin binding at low unlabelled-insulin concentrations but no change in receptor numbers (Cushing's 180,000 +/- 48,000 (3) receptors/cell and controls 189,000 +/- 30,000 (7)) together with a fourfold decrease in apparent receptor affinity (ED50: Cushing's 2.25 x 10(-9) M and controls 0.57 x 10(-9) M) and a decreased sensitivity to the antilipolytic effect of insulin. These events could represent the final situation of a chronic and endogenous regulation by high levels of cortisol of insulin receptors in human adipose tissue.
Calle
C
C
Department of Biochemistry, School of Medicine, Complutese University, Madrid, Spain.
Carranza
M C
MC
Simón
M A
MA
Torres
A
A
Mayor
P
P
D000273:Adipose Tissue / Q000378:metabolism / Q000473:pathology*; D000328:Adult; D003480:Cushing Syndrome / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007328:Insulin / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D011972:Receptor, Insulin / Q000378:metabolism*
D007328:Insulin; D011972:Receptor, Insulin