Insulin, ketones, glucose and glucagon: effects on the arterial basement membrane in vitro.
The influence of insulin in physiological concentrations on basement membrane (BM)-like material from arterial smooth muscle cells was studied in cell cultures. BM-like material was isolated from the cell layer by a sonication-differential centrifugation technique after a 24-h incubation period with insulin and [3H]leucine added to the culture medium. Insulin in concentrations of 50, 100, 200 and 500 mU/l was found to exert no influence on incorporation of amino acids into BM-like material. Neither glucose, 16 mmol/l, ketones, 1 mmol/l, nor glucagon 100 ng/l were able to induce altered accumulation of BM-like material. The effect of insulin on the carbohydrate units of BM-like material was studied by concanavalin A-Sepharose chromatography of glycopeptides and by analyses of neutral hexoses and amino sugars. The result showed that insulin, 200 mU/l, induced a shift in composition of neutral sugars by decreasing the amount of galactose relative to glucose and mannose. No changes in glycopeptide binding to the concanavalin A-sepharose column were observed. The results indicate that insulin induces only minor alterations in BM-like material of arterial smooth muscle cells and that the changes apparently are restricted to the carbohydrate units.
Ledet
T
T
Heickendorff
L
L
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D001158:Arteries / Q000378:metabolism*; D001485:Basement Membrane / Q000378:metabolism; D050260:Carbohydrate Metabolism; D002478:Cells, Cultured; D005934:Glucagon / Q000494:pharmacology*; D005947:Glucose / Q000494:pharmacology*; D006020:Glycopeptides / Q000378:metabolism; D007328:Insulin / Q000494:pharmacology*; D007659:Ketones / Q000494:pharmacology*; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000378:metabolism*; D011817:Rabbits
D000596:Amino Acids; D006020:Glycopeptides; D007328:Insulin; D007659:Ketones; D005934:Glucagon; D005947:Glucose