Effects of Bordetella avium infection on the pulmonary clearance of Escherichia coli in turkeys.
Thirty-six 1-day-old turkeys were inoculated intranasally with Bordetella avium (BA) strain 838. Noninoculated hatchmates (n = 36) were housed separately. At 2 and 4 weeks of age, 15 inoculated (BA+) and 15 noninoculated (BA-) turkeys were exposed to an aerosol of virulent Escherichia coli. The remaining six BA+ turkeys and six BA- turkeys were used as controls (ie, not exposed to E coli). Turkeys were necropsied on postaerosolization days 0 (immediately after aerosolization), 1, 3, 5, and 7. Lung and tracheal specimens were collected from each turkey for bacterial quantitation and histologic examination. A 1-ml blood sample was collected for detection of bacteremia. Numbers of E coli in lung specimens from 2- and 4-week-old turkeys were not significantly different between BA+ and BA- groups (pooled data over time); however, numbers of E coli isolated from tracheal specimens were significantly greater in BA+ turkeys than those in BA- turkeys. Although the incidence of pulmonary abcesses and E coli bacteremia was greater in 2-week-old turkeys than in 4-week-old turkeys, the incidence was not different between BA+ and BA- turkeys. At both ages, air sacculitis developed more often and was more severe in BA+ turkeys than in BA- turkeys. Hyperplastic bronchus-associated lymphoid tissue was found more often in BA+ turkeys than in BA- turkeys and appeared to be the first site of heterophil infiltration after E coli aerosolization.
Van Alstine
W G
WG
Arp
L H
LH
D000818:Animals; D001884:Bordetella / Q000254:growth & development; D001885:Bordetella Infections / Q000150:complications / Q000382:microbiology / Q000662:veterinary*; D004926:Escherichia coli / Q000254:growth & development; D004927:Escherichia coli Infections / Q000150:complications / Q000382:microbiology / Q000662:veterinary*; D008168:Lung / Q000382:microbiology / Q000473:pathology; D011201:Poultry Diseases / Q000382:microbiology*; D012141:Respiratory Tract Infections / Q000382:microbiology / Q000662:veterinary*; D018805:Sepsis / Q000382:microbiology / Q000662:veterinary; D014132:Trachea / Q000382:microbiology / Q000473:pathology; D014422:Turkeys