title: Reversion of 7-methylguanosine 5'-phosphate inhibition of mRNA translation by polysomal and soluble factors isolated from Saccharomyces cerevisiae.
abstract: Protein fractions that overcome m7GMP inhibition of mRNA translation have been purified from the yeast S. cerevisiae. An active fraction isolated from polysomes contains two polypeptides of 220- and 190-kDa. The active fraction isolated from postribosomal supernatant contains a major polypeptide of 28-kDa and other species of 32-, 24-, 22- and 21-kDa, and sediments in sucrose gradients as a high molecular weight complex of about 200,000. This fraction restored yeast mRNA translation in reticulocyte lysates under conditions of yeast and globin mRNA competition; however, this effect was not observed with the 220- and 190-kDa polypeptides from polysomes. Nevertheless, translation of yeast mRNA was stimulated by a partially purified fraction containing a 28-kDa polypeptide from polysomes.
authors.forename: A
authors.initials: A
authors.lastname: Parets Suler
authors.forename: J
authors.initials: J
authors.lastname: Zueco
authors.forename: D
authors.initials: D
authors.lastname: Gozalbo
authors.forename: R
authors.initials: R
authors.lastname: Sentandreu
mesh_terms: D005656:Fungal Proteins / Q000502:physiology; D007700:Kinetics; D011132:Polyribosomes / Q000187:drug effects / Q000378:metabolism*; D014176:Protein Biosynthesis / Q000187:drug effects*; D012314:RNA Cap Analogs / Q000494:pharmacology*; D012315:RNA Caps / Q000494:pharmacology*; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism
chemical_list: D005656:Fungal Proteins; D012314:RNA Cap Analogs; D012315:RNA Caps; D012333:RNA, Messenger; C011925:7-methylguanosine-5'-monophosphate