Salmonella typhimurium deletion mutants lacking adenylate cyclase and cyclic AMP receptor protein are avirulent and immunogenic.
Salmonella typhimurium SR-11 mutants with cya::Tn10 or crp::Tn10 mutations were found to be avirulent and immunogenic for BALB/c mice. Fusaric acid-resistant derivatives with deletions of the Tn10 and adjacent DNA sequences were constructed in S. typhimurium SR-11 strains with or without the virulence plasmid pStSR100. These delta cya delta crp strains grew more slowly than wild-type strains. They possessed wild-type ability to attach to, invade, and persist in gut-associated lymphoid tissue for up to a week but exhibited a diminished ability to reach mesenteric lymph nodes and the spleen. Mice 4 to 8 weeks old were resistant to oral infection with 10(9) cells of several different delta cya and delta cya delta crp strains (the equivalent to 10(4) 50% lethal doses of wild-type S. typhimurium SR-11) and 30 days after immunization became resistant to oral challenge with 10(3) to 10(4) 50% lethal doses of wild-type S. typhimurium SR-11.
Curtiss
R
R
Department of Biology, Washington University, St. Louis, Missouri 63130.
Kelly
S M
SM
D000262:Adenylyl Cyclases / Q000502:physiology*; D000284:Administration, Oral; D000818:Animals; D007114:Immunization; D008198:Lymph Nodes / Q000382:microbiology; D051379:Mice; D009154:Mutation; D010581:Peyer's Patches / Q000382:microbiology; D011953:Receptors, Cyclic AMP / Q000502:physiology*; D012481:Salmonella Infections, Animal / Q000382:microbiology / Q000517:prevention & control; D012486:Salmonella typhimurium / Q000235:genetics / Q000276:immunology / Q000472:pathogenicity*; D014018:Tissue Distribution
D011953:Receptors, Cyclic AMP; D000262:Adenylyl Cyclases