title: Chemical and biological characterization of hazardous industrial waste. II. Eukaryotic bioassay of a wood-preserving bottom sediment.
abstract: The eukaryotic haploid and diploid forms of Aspergillus nidulans were used to detect gene mutations and various types of chromosome damage, respectively, in the acid, base and neutral fractions of a wood-preserving bottom sediment. The corresponding response to prokaryotic mutagenicity assays and major chemical constituents of the 3 waste fractions were described by Donnelly et al. (1987). The haploid methionine system detected genotoxic compounds in all 3 primary waste fractions without metabolic activation. With metabolic activation, the maximum response observed in the gene mutation assay was induced by the base fraction. In the diploid assay without metabolic activation, the acid fraction induced the maximum number of major chromosome abnormalities, while the base fraction induced the maximum number of minor deletions or insertions. These results appear to reflect the different composition of the waste fractions since each fraction induced a different type of genetic damage in the two bioassays employed. Alternately, because exposure in the diploid assay was during a growth stage, the results may reflect a varying response at different points of the cell division cycle. The results obtained using eukaryotic bioassays indicate that the wood preserving waste contains compound(s) capable of inducing point mutations, chromosome damage, recombination, and compound(s) acting as spindle poisons.
authors.forename: K C
authors.initials: KC
authors.lastname: Donnelly
authors.forename: K W
authors.initials: KW
authors.lastname: Brown
authors.forename: B R
authors.initials: BR
authors.lastname: Scott
mesh_terms: D001233:Aspergillus nidulans / Q000187:drug effects*; D001681:Biological Assay; D004305:Dose-Response Relationship, Drug; D006238:Haploidy; D006863:Hydrogen-Ion Concentration; D007220:Industrial Waste / Q000032:analysis; D009152:Mutagenicity Tests; D009153:Mutagens / Q000032:analysis*; D014934:Wood
chemical_list: D007220:Industrial Waste; D009153:Mutagens