title: Effects of chronic renal failure on the regulation of pyruvate kinase.
abstract: The effects of chronic renal failure on the enzyme activity of pyruvate kinase and the mRNA level of this enzyme were studied in 7 out of 8 nephrectomized rats. The mRNA level was measured by RNA-DNA dot blot hybridization, using cloned pyruvate kinase cDNA as hybridized probe. Neither the activity of M1-type pyruvate kinase nor the level of this enzyme in rat gastrocnemius muscle was affected by chronic renal failure, whereas L-type pyruvate kinase enzyme activity in uremic rat liver was lower than that in control at both fasted and refed states. The levels of L-type pyruvate kinase mRNA were not different between two groups at the fasted state. Induction of L-type pyruvate kinase mRNA after high carbohydrate diet refeeding was suppressed proportionally to the severity of chronic renal failure, which was expressed by the serum creatinine concentrations (r = -.876, P less than .005). These results indicate that the suppression of L-type pyruvate kinase activity in uremia was partly reflected by the decreased accumulation of this enzyme mRNA. There was a significantly negative correlation between L-type pyruvate kinase mRNA levels and plasma glucagon/insulin ratios (r = -.719, P less than .05). Hyperglucagonemia in uremia might play a major role in this suppression.
authors.forename: E
authors.initials: E
authors.lastname: Imai
authors.forename: A
authors.initials: A
authors.lastname: Yamauchi
authors.forename: T
authors.initials: T
authors.lastname: Noguchi
authors.forename: T
authors.initials: T
authors.lastname: Tanaka
authors.forename: M
authors.initials: M
authors.lastname: Fujii
authors.forename: H
authors.initials: H
authors.lastname: Mikami
authors.forename: Y
authors.initials: Y
authors.lastname: Fukuhara
authors.forename: A
authors.initials: A
authors.lastname: Ando
authors.forename: Y
authors.initials: Y
authors.lastname: Orita
authors.forename: T
authors.initials: T
authors.lastname: Kamada
mesh_terms: D000818:Animals; D003404:Creatinine / Q000097:blood; D005934:Glucagon / Q000097:blood; D007328:Insulin / Q000097:blood; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D007676:Kidney Failure, Chronic / Q000201:enzymology*; D008099:Liver / Q000201:enzymology; D008297:Male; D009132:Muscles / Q000201:enzymology; D009693:Nucleic Acid Hybridization; D011770:Pyruvate Kinase / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D014511:Uremia / Q000201:enzymology
chemical_list: D007328:Insulin; D007527:Isoenzymes; D012333:RNA, Messenger; D005934:Glucagon; D003404:Creatinine; D011770:Pyruvate Kinase