Influence of cell sources, stimulating agents, and incubation conditions on release of interleukin-1 from chicken macrophages.
Experiments were conducted to determine the cell source, stimulating agents, and incubation conditions that maximize interleukin-1 (IL-1) release by chicken macrophages/monocytes. Thymocyte co-mitogen proliferation was used to assay IL-1 activity of conditioned or partially purified supernatants. Monolayers of a transformed chicken macrophage cell line, HD11, released greater amounts of IL-1 than adherent cells isolated from peripheral blood, peritoneal cavity, or spleen. E. coli endotoxin and heat-killed S. aureus induced greater release of IL-1 by HD11 and splenic macrophages than latex or a super induction protocol with mezerien. Blocking macrophage eicosanoid synthesis with indomethacin did not influence IL-1 release from HD11 macrophages. Removing low molecular weight compounds from conditioned supernatants by dialysis did not influence IL-1 activity. IL-1 release was increased by incubating macrophages at 42 C compared to 39 C. Thymocyte co-mitogenic activity of IL-1 was increased by incubating thymocytes at 42 C compared to 39 C. Species cross reactivity between chicken and mammalian IL-1 was also investigated. Chicken IL-1 had slight co-stimulation activity on murine thymocytes, but murine and human IL-1 were without activity on chicken thymocytes.
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D000818:Animals; D002645:Chickens / Q000276:immunology*; D003429:Cross Reactions; D004731:Endotoxins / Q000276:immunology; D004926:Escherichia coli; D006801:Humans; D066298:In Vitro Techniques; D007213:Indomethacin / Q000494:pharmacology; D007375:Interleukin-1 / Q000378:metabolism*; D008262:Macrophage Activation / Q000187:drug effects; D008264:Macrophages / Q000187:drug effects / Q000276:immunology*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D013045:Species Specificity; D013211:Staphylococcus aureus / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D013696:Temperature
D004731:Endotoxins; D007375:Interleukin-1; D007213:Indomethacin