title: Monoclonal antibodies to bovine collagenase inhibitor.
abstract: Hybridoma antibodies against bovine collagenase inhibitor were produced by fusion of myeloma cells NS-1 (P3-NS1-1) with spleen cells from mice hyperimmunized with collagenase inhibitor purified from the explant medium of bovine dental pulps. Hybridomas positive by an enzyme-linked immunosorbent assay (ELISA) for bovine collagenase inhibitor were cloned by the dilution method. Seventeen hybridomas producing antibodies were isolated, four of which also recognized purified human collagenase inhibitor in the ELISA. Using a monoclonal antibody-Sepharose affinity column, we easily purified both bovine and human collagenase inhibitors to homogeneity. They showed the same mobility on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, corresponding to a molecular mass of 32,000 daltons.
authors.affiliation: Fuji Chemical Industries, Ltd., Takaoka, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Kodama
authors.forename: J
authors.initials: J
authors.lastname: Kishi
authors.forename: K
authors.initials: K
authors.lastname: Obata
authors.forename: K
authors.initials: K
authors.lastname: Iwata
authors.forename: T
authors.initials: T
authors.lastname: Hayakawa
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis*; D001013:Aorta, Thoracic / Q000032:analysis; D002417:Cattle; D003782:Dental Pulp / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D004791:Enzyme Inhibitors / Q000276:immunology*; D005260:Female; D006825:Hybridomas / Q000378:metabolism; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D007163:Immunosorbent Techniques; D051379:Mice; D009101:Multiple Myeloma / Q000276:immunology; D012967:Sodium Dodecyl Sulfate; D013154:Spleen / Q000166:cytology; D019714:Tissue Inhibitor of Metalloproteinases
chemical_list: D000911:Antibodies, Monoclonal; D004791:Enzyme Inhibitors; D019714:Tissue Inhibitor of Metalloproteinases; D012967:Sodium Dodecyl Sulfate