Immunoelectron microscopic study of glomerular lesions using a postembedding method with a protein A-gold complex.
Renal biopsy tissue from 33 children with various glomerular diseases has been investigated by electron microscopy using a postembedding immunostaining technique with a protein A-gold complex in order to establish more precise correlations between immunopathologic and morphologic findings in glomeruli. This technique could detect immunoglobulins (IgG, IgA, and IgM), complement factor (C3c), and fibrinogen-related antigen. The immunoreactivity of these antigens was essentially confined to the mesangial, paramesangial, subendothelial, and subepithelial 'electron-dense deposits' in the glomeruli. Except for IgM and C3c in the case of glomerular sclerosis, the distribution of the mentioned factors was even in the electron-dense deposits, as could be shown by 'double-immunolabeling'. From the above-mentioned findings one can conclude that several of the localized factors are associated with the formation of electron-dense deposits, the ultrastructural hallmarks of glomerular disease.
Nakajima
M
M
Hirota
T
T
Kusumoto
K
K
Taira
K
K
Kamitsuji
H
H
D000941:Antigens / Q000032:analysis; D003176:Complement C3 / Q000032:analysis; D015932:Complement C3c; D006046:Gold; D006801:Humans; D007136:Immunoglobulins / Q000032:analysis; D007158:Immunologic Techniques; D007674:Kidney Diseases / Q000276:immunology / Q000473:pathology*; D007678:Kidney Glomerulus / Q000276:immunology / Q000473:pathology; D008854:Microscopy, Electron / Q000379:methods; D008856:Microscopy, Fluorescence / Q000379:methods; D013205:Staphylococcal Protein A
D000941:Antigens; D003176:Complement C3; D007136:Immunoglobulins; D013205:Staphylococcal Protein A; C023456:fibrinogen-related antigen; D006046:Gold; D015932:Complement C3c