Monoamine oxidase in rat and bovine endocrine tissues.
Monoamine oxidase (MAO) was characterized in tissue homogenates from rat pancreatic islets, rat neurohypophysis and adenohypophysis, and rat and bovine adrenal medulla and adrenal cortex. Phenylethylamine was preferentially deaminated by rat pancreatic islet and bovine adrenal medulla MAO and with slight preference by rat neurohypophysis MAO, whereas 5-hydroxytryptamine was preferentially deaminated by MAO from all other endocrine tissues. Tyramine was a good substrate for all tissues. Clorgyline, a selective inhibitor of MAO-A, preferentially inhibited deamination of 5-hydroxytryptamine by all tissue homogenates, whereas deprenyl, a selective inhibitor of MAO-B, preferentially inhibited deamination of phenylethylamine. Km values for 5-hydroxytryptamine and tyramine were higher by one to two decimal powers than for phenylethylamine in homogenates from all endocrine tissues. Km values were significantly lower for 5-hydroxytryptamine and significantly higher for phenylethylamine in rat and bovine adrenal cortex than in adrenal medulla. According to these results, the contributions of MAO-B to total enzyme activity were 70% for rat pancreatic islets, 45% for rat neurohypophysis, 15% for rat adenohypophysis, 20% for rat adrenal medulla, 10% for rat adrenal cortex, 60% for bovine adrenal medulla, and 20% for bovine adrenal cortex. PC 12 cells also contained predominantly MAO-A (90%); however, an increased Km for phenylethylamine and a sensitivity of deamination of this MAO-B substrate to inhibition by clorgyline are indicators of abnormal behavior of MAO in this clonal rat pheochromocytoma cell line.
Lenzen
S
S
Freisinger-Treichel
M
M
Panten
U
U
D000302:Adrenal Cortex / Q000201:enzymology; D000310:Adrenal Gland Neoplasms / Q000201:enzymology; D000313:Adrenal Medulla / Q000201:enzymology; D000818:Animals; D002417:Cattle; D002460:Cell Line; D004702:Endocrine Glands / Q000201:enzymology*; D005260:Female; D007515:Islets of Langerhans / Q000201:enzymology; D007700:Kinetics; D008995:Monoamine Oxidase / Q000378:metabolism*; D010673:Pheochromocytoma / Q000201:enzymology; D010903:Pituitary Gland, Anterior / Q000201:enzymology; D010904:Pituitary Gland, Posterior / Q000201:enzymology; D051381:Rats; D011919:Rats, Inbred Strains; D013379:Substrate Specificity; D014018:Tissue Distribution
D008995:Monoamine Oxidase