title: Enzyme-linked immunofiltration assay for rapid serodiagnosis of syphilis.
abstract: A new rapid technique for detection of serum treponemal antibodies is described which is based on an enzyme-linked immunoassay using nitrocellulose as solid phase. With this technique antigen-antibody binding is accelerated by the filtration of the antibody solution through the antigen-coated nitrocellulose filter instead of its remaining over the solid phase for incubation. Test results are available in less than 15 min. Serum specimens from 255 syphilitics and 829 non-infected subjects were investigated. The sensitivity and specificity of the Treponema pallidum enzyme-linked immunofiltration assay were comparable to those of the Treponema pallidum haemagglutination assay and the fluorescent treponemal antibody absorption test.
authors.forename: O E
authors.initials: OE
authors.lastname: Ijsselmuiden
authors.forename: M M
authors.initials: MM
authors.lastname: Meinardi
authors.forename: J J
authors.initials: JJ
authors.lastname: van der Sluis
authors.forename: H E
authors.initials: HE
authors.lastname: Menke
authors.forename: E
authors.initials: E
authors.lastname: Stolz
authors.forename: R V
authors.initials: RV
authors.lastname: van Eijk
mesh_terms: D000907:Antibodies, Bacterial / Q000032:analysis*; D000942:Antigens, Bacterial / Q000276:immunology; D003101:Collodion; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005374:Filtration; D005455:Fluorescent Antibody Technique; D006386:Hemagglutination Tests; D006801:Humans; D008297:Male; D011237:Predictive Value of Tests; D013588:Syphilis Serodiagnosis; D014210:Treponema pallidum / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D003101:Collodion