title: Oxytocin in baboon (Papio anubis) corpus luteum.
abstract: Recently, using a highly specific radioimmunoassay, we have demonstrated that the concentration of oxytocin in the corpus luteum of the human and cynomolgus monkey are several fold higher than in the peripheral circulation. In this study, we have examined the corpora lutea and ovarian stroma from the ovaries of normal adult cycling baboons (Papio anubis) for the presence of oxytocin through the use of immunocytochemical procedures. Tissues obtained at laparotomy were fixed in Bouin's solution and embedded in paraffin; immunoreactive oxytocin was localized with peroxidase-antiperoxidase and 3.3' diaminobenzidine. Six corpora lutea with stroma were obtained--two each from the early (Day 14-20), mid-(Day 21-24), and late (Day 25-30) stages of the luteal phase. Immunoreactive oxytocin was localized in all corpora lutea examined but was absent from all stroma samples. Larger areas of the corpus luteum from the mid-luteal phase showed staining for oxytocin, and the intensity of staining for this peptide was maximal in this phase of the cycle.
authors.affiliation: Department of Obstetrics and Gynecology, University of Illinois College of Medicine, Chicago 60612.
authors.forename: F S
authors.initials: FS
authors.lastname: Khan-Dawood
mesh_terms: D000818:Animals; D003338:Corpus Luteum / Q000032:analysis*; D005260:Female; D007124:Immunoenzyme Techniques; D008183:Luteal Phase; D010121:Oxytocin / Q000032:analysis*; D010215:Papio
chemical_list: D010121:Oxytocin