title: Development of a practicable method for isolation and identification of dengue viruses in developing countries.
abstract: We sought a practicable method for isolation and identification of dengue viruses in South-East Asia. We compared two mosquito cell lines, C6/36 and TRA-284-SFG, for virus isolation and two identification methods, immunofluorescent staining of infected cells with serotype-specific mouse monoclonal antibodies and a sandwich-type enzyme-linked immunosorbent assay with conventional mouse hyperimmune ascitic fluids. We found that the combination of TRA-284-SFG cells and ELISA is a useful and feasible method in developing countries.
authors.affiliation: Department of Microbiology, Saitama Medical School, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Nawa
authors.forename: C A
authors.initials: CA
authors.lastname: Torres
authors.forename: F J
authors.initials: FJ
authors.lastname: Paladin
authors.forename: T E
authors.initials: TE
authors.lastname: Tupasi
authors.forename: Y
authors.initials: Y
authors.lastname: Kaneko
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000302:isolation & purification; D002460:Cell Line; D003715:Dengue / Q000175:diagnosis; D003716:Dengue Virus / Q000276:immunology / Q000302:isolation & purification*; D003906:Developing Countries; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006801:Humans; D014776:Virus Cultivation / Q000379:methods
chemical_list: D000956:Antigens, Viral