title: Quantitative determination of the lectin binding capacity of small intestinal brush-border membrane. An enzyme linked lectin sorbent assay (ELLSA).
abstract: A test to determine quantitatively the lectin binding sites in brush-border membranes has been developed. Highly purified bovine small intestinal brush-border membranes were prepared, and subsequently coated directly to the bottom of a microtiter plate. Soybean agglutinin conjugated with peroxidase was coupled to its binding sites in the brush-border membranes and the peroxidase activity was determined in a spectrophotometer. The number of soybean agglutinin binding sites in the brush-border membranes has been established by means of iterized computer fit analysis of the data, indicating values for maximal binding of 7.10(-7) M soybean agglutinin per mg of brush-border membrane protein and a dissociation constant of 1.5.10(-5) M.
authors.affiliation: Department of Veterinary Pathology, Faculty of Veterinary Medicine, University of Utrecht, The Netherlands.
authors.forename: H G
authors.initials: HG
authors.lastname: Hendriks
authors.forename: J F
authors.initials: JF
authors.lastname: Koninkx
authors.forename: M
authors.initials: M
authors.lastname: Draaijer
authors.forename: J E
authors.initials: JE
authors.lastname: van Dijk
authors.forename: J A
authors.initials: JA
authors.lastname: Raaijmakers
authors.forename: J M
authors.initials: JM
authors.lastname: Mouwen
mesh_terms: D000818:Animals; D002417:Cattle; D007124:Immunoenzyme Techniques; D007163:Immunosorbent Techniques; D007421:Intestine, Small / Q000378:metabolism*; D037102:Lectins / Q000378:metabolism*; D008871:Microvilli / Q000378:metabolism*; D037121:Plant Lectins; D011975:Receptors, Mitogen / Q000032:analysis*; D030262:Soybean Proteins; D010835:Phytohemagglutinins
chemical_list: D037102:Lectins; D037121:Plant Lectins; D011975:Receptors, Mitogen; D030262:Soybean Proteins; C028824:soybean lectin; D010835:Phytohemagglutinins; C046772:soybean lectin receptor