title: Performance of the two-site immunoradiometric assay for serum thyroid-stimulating hormone. Effects of changes in solid-phase matrix and antibody coupling chemistry.
abstract: Ten solid-phases were evaluated for their usefulness in a two-site immunoradiometric assay for serum thyroid-stimulating hormone. The criteria used to assess each reagent included the minimum detection limit attainable, the change of binding over the concentration range (signal: noise ratio), and ease of preparation of the reagents. Of all the solid phases tested, Sepharose CL-4B and activated CH-Sepharose 4B showed the characteristics best suited to IRMA, while epoxy-activated Sepharose 6B and Ultrogel ACA-44 were marginally less effective.
authors.affiliation: Department of Pathological Biochemistry, Royal Infirmary, Glasgow, U.K.
authors.forename: M G
authors.initials: MG
authors.lastname: McConway
authors.forename: E M
authors.initials: EM
authors.lastname: Biggart
authors.forename: R S
authors.initials: RS
authors.lastname: Chapman
mesh_terms: D000911:Antibodies, Monoclonal; D006801:Humans; D007120:Immunochemistry; D007163:Immunosorbent Techniques; D011863:Radioimmunoassay / Q000379:methods*; D013972:Thyrotropin / Q000032:analysis*
chemical_list: D000911:Antibodies, Monoclonal; D013972:Thyrotropin