title: Primary structure of lymphocyte function-associated antigen 3 (LFA-3). The ligand of the T lymphocyte CD2 glycoprotein.
abstract: We have isolated the cDNA for human lymphocyte function-associated antigen 3 (LFA-3), the ligand of the T lymphocyte CD2 molecule. The identity of the clones was established by comparison of the deduced amino acid sequence to the LFA-3 NH2-terminal and tryptic peptide sequences. The cDNA defines a mature protein of 222 amino acids that structurally resembles typical membrane-anchored proteins. An extracellular domain with six N-linked glycosylation sites is followed by a hydrophobic putative transmembrane region and a short cytoplasmic domain. The mature glycoprotein is estimated to be 44-68% carbohydrate. Southern blots of human genomic DNA indicate that only one gene codes for human LFA-3. Northern blot analysis demonstrates that the LFA-3 mRNA of 1.3 kb is widely distributed in human tissues and cell lines.
authors.affiliation: Biogen Research Corporation, Cambridge, Massachusetts 02142.
authors.forename: B P
authors.initials: BP
authors.lastname: Wallner
authors.forename: A Z
authors.initials: AZ
authors.lastname: Frey
authors.forename: R
authors.initials: R
authors.lastname: Tizard
authors.forename: R J
authors.initials: RJ
authors.lastname: Mattaliano
authors.forename: C
authors.initials: C
authors.lastname: Hession
authors.forename: M E
authors.initials: ME
authors.lastname: Sanders
authors.forename: M L
authors.initials: ML
authors.lastname: Dustin
authors.forename: T A
authors.initials: TA
authors.lastname: Springer
mesh_terms: D000595:Amino Acid Sequence; D000945:Antigens, Differentiation, T-Lymphocyte / Q000378:metabolism*; D000954:Antigens, Surface / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis; D006801:Humans; D016169:Lymphocyte Function-Associated Antigen-1; D008969:Molecular Sequence Data; D010449:Peptide Mapping
chemical_list: D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface; D016169:Lymphocyte Function-Associated Antigen-1; D004247:DNA