Mutants of polyomavirus middle-T antigen.
Polyomavirus middle-T antigen induces the transformation of established cell lines in culture and is known to interact with and/or modulate the activity of several enzymes (pp60c.src, protein kinase C and phosphatidylinositol kinase) in vitro. This review is a compilation of the reported mutants of middle-T antigen and their biochemical and biological properties as they relate to the transformation event. The mutants of polyomavirus middle-T antigen have been previously classified phenotypically. Given the now large number of mutants, the classification presented here is based upon the position within the molecule. A model of middle-T is presented in which the protein is considered as consisting of three domains: a hydrophobic domain (the putative membrane-binding domain), the amino-terminal half of the molecule (the putative pp60c.src-binding domain) and the intervening amino acids (the putative modulatory domain). A current model for the induction of transformation by polyomavirus middle-T is presented.
Markland
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Integrated Genetics, Framingham, MA 01701.
Smith
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AE
D000595:Amino Acid Sequence; D002471:Cell Transformation, Neoplastic; D002872:Chromosome Deletion; D008954:Models, Biological; D009154:Mutation; D009856:Oncogene Proteins, Viral / Q000235:genetics*
D009856:Oncogene Proteins, Viral