Purification of trypsin and bacterial proteinases by column chromatography on coffee grain particles.
Trypsin and extracellular proteinases produced by Bacillus sp. were purified by column chromatography on coffee grain particles. The ballast proteins were eluted with water, while the adsorbed proteinases were eluted with 1 M sodium chloride solution. The capacity is approximately 2 mg of trypsin per ml of the packed sorbent.
Safarík
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D001407:Bacillus / Q000201:enzymology*; D002845:Chromatography / Q000379:methods; D002851:Chromatography, High Pressure Liquid; D003069:Coffee; D010450:Endopeptidases / Q000302:isolation & purification*; D014357:Trypsin / Q000302:isolation & purification*
D003069:Coffee; D010450:Endopeptidases; D014357:Trypsin