Autoradiographic evidence of [3H]SCH 23390 binding sites in human prefrontal cortex (Brodmann's area 9).
The distribution of dopamine D-1 receptors has been determined in human prefrontal cortex (Brodmann's area 9) by an in vitro light microscopic autoradiographic method. Dopamine D-1 receptors were localized by using [3H]SCH 23390 as a ligand. Our results indicated that [3H]SCH 23390 binding to slide-mounted tissue sections of human brain is specific, saturable, and of high affinity. Lamina Va contained the highest density of D-1 receptors, with a Bmax value of 11.2 +/- 1.3 fmol/mg tissue. The KD values for [3H]SCH 23390 in all laminae ranged from 2.6 to 3.2 nM. Competition studies performed with [3H]SCH 23390 indicated a pharmacologic profile consistent with labeling of the D-1 receptor.
Dawson
T M
TM
McCabe
R T
RT
Stensaas
S S
SS
Wamsley
J K
JK
D001345:Autoradiography; D001552:Benzazepines / Q000378:metabolism*; D001665:Binding Sites; D002540:Cerebral Cortex / Q000378:metabolism*; D003198:Computer Simulation; D006801:Humans; D007700:Kinetics; D011954:Receptors, Dopamine / Q000378:metabolism; D013237:Stereoisomerism; D014018:Tissue Distribution
D001552:Benzazepines; D011954:Receptors, Dopamine