Clonal immunoglobulin gene rearrangements in tissues involved by Hodgkin's disease.
The nature of Reed-Sternberg cells, the abnormal cells of Hodgkin's disease, is controversial. Morphological and immunologic marker studies suggested different cells of origin. To investigate a possible B or T cell origin, immunoglobulin and T cell receptor gene analyses were performed on tissues from 11 patients in early and late stages of Hodgkin's disease. In addition, the immunologic marker patterns of the Reed-Sternberg cells were determined. Rearrangements of immunoglobulin heavy- and light-chain genes were detected in tissues from five patients, particularly in late stages of the disease when lymphocyte depletion had occurred. No rearrangements of T cell receptor genes were found. The results indicate that clonal immunoglobulin gene rearrangements can be detected in tissues involved by Hodgkin's disease.
Brinker
M G
MG
Poppema
S
S
Buys
C H
CH
Timens
W
W
Osinga
J
J
Visser
L
L
D000293:Adolescent; D000328:Adult; D000954:Antigens, Surface / Q000032:analysis; D002869:Chromosome Aberrations; D004247:DNA / Q000378:metabolism; D005260:Female; D005796:Genes; D006644:Histiocytes / Q000473:pathology; D006651:Histocytochemistry; D006689:Hodgkin Disease / Q000473:pathology*; D006801:Humans; D007124:Immunoenzyme Techniques; D007136:Immunoglobulins / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009693:Nucleic Acid Hybridization; D011948:Receptors, Antigen, T-Cell / Q000235:genetics; D014018:Tissue Distribution
D000954:Antigens, Surface; D007136:Immunoglobulins; D011948:Receptors, Antigen, T-Cell; D004247:DNA