Escherichia coli strains carrying the cloned cytochrome d terminal oxidase complex are sensitive to near-UV inactivation.
To determine if membrane-bound cytochromes function as endogenous near-UV photosensitizers, strains containing the cloned cydA and cydB genes were tested for near-UV sensitivity. A strain containing both cloned genes overproduced cytochromes b558, b595, and d. Another strain containing only cloned cydB overproduced cytochrome b558. Both cytochrome-overproducing strains were hypersensitive to broad-spectrum near-UV inactivation. The presence of excess cytochromes did not affect sensitivity to far-UV radiation and provided protection against H2O2 inactivation.
Sammartano
L J
LJ
Department of Microbiology, University of Illinois, Urbana 61801.
Tuveson
R W
RW
D002462:Cell Membrane / Q000201:enzymology; D003001:Cloning, Molecular; D003573:Cytochrome b Group; D003580:Cytochromes; D004307:Dose-Response Relationship, Radiation; D045222:Electron Transport Chain Complex Proteins; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics / Q000528:radiation effects*; D029968:Escherichia coli Proteins; D010088:Oxidoreductases / Q000235:genetics*; D010957:Plasmids; D014466:Ultraviolet Rays
D003573:Cytochrome b Group; D003580:Cytochromes; D045222:Electron Transport Chain Complex Proteins; D029968:Escherichia coli Proteins; D010088:Oxidoreductases; C040077:cytochrome bd terminal oxidase complex, E coli