Effect of oxidation on the conformation of cell surface H-2 antigens detected by monoclonal antibodies.
Periodate treatment of lymphoid cells is known to render them susceptible to nonspecific lysis by cytotoxic T lymphocytes. To detect alterations in cell surface molecules as a result of such treatment, we investigated the binding of antibodies to oxidized and nonoxidized lymphoid cells and found that oxidation selectively decreases the binding of some anti-H-2 and anti-beta 2m antibodies. This effect was not caused by loss of H-2 molecules from the cells, since the rate of exchange of cell surface beta 2m by exogenously added beta 2m was unchanged, and neither H-2 nor beta 2m molecules could be detected in the supernates of oxidized cells. Our findings suggest that oxidation alters the conformation of H-2 antigens in such a way as to reduce antibody binding. Such conformational changes may further affect the recognition of histocompatibility antigens by cytotoxic T cells.
Keren
Z
Z
Department of Immunology, University of Texas M.D. Anderson Hospital and Tumor Institute, Houston 77030.
Berke
G
G
Robinson
P J
PJ
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D006183:H-2 Antigens / Q000032:analysis*; D066298:In Vitro Techniques; D051379:Mice; D010084:Oxidation-Reduction; D010100:Oxygen; D011487:Protein Conformation; D014407:Tumor Cells, Cultured; D001613:beta 2-Microglobulin / Q000493:pharmacokinetics
D000911:Antibodies, Monoclonal; D006183:H-2 Antigens; D001613:beta 2-Microglobulin; D010100:Oxygen