[Isolation and analysis of human leukocyte interferons synthesized by bacterial producer strains].
Rapid and sensitive methods for immunochemical estimation of bacterially-made human leukocytic interferons were developed. A large number of strains producing such interferons was analyzed with these methods and optimal ones capable of synthesizing up to 10(10), 10(8), 10(9) and 10(9) IU/l of interferons alpha A, alpha N, alpha F and alpha I1 respectively were selected. The proteins were isolated in homogenous state and their main physico-chemical parameters were characterized. Interferon oligomers were detected. Their formation was partially due to intermolecular disulphide exchange. The effect on interferon alpha A of retinal (vitamin A) and its derivatives efficiently interacting with this protein was studied.
Kostrov
S V
SV
Borukhov
S I
SI
Eremashvili
M R
MR
Novikov
A A
AA
Skvortsova
M A
MA
D001419:Bacteria / Q000378:metabolism*; D001426:Bacterial Proteins / Q000302:isolation & purification; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007124:Immunoenzyme Techniques; D007163:Immunosorbent Techniques; D007370:Interferon Type I / Q000032:analysis / Q000096:biosynthesis / Q000302:isolation & purification*; D011489:Protein Denaturation; D011994:Recombinant Proteins / Q000032:analysis / Q000096:biosynthesis / Q000302:isolation & purification; D013696:Temperature
D001426:Bacterial Proteins; D007370:Interferon Type I; D011994:Recombinant Proteins
Poluchenie i analiz riada chelovecheskikh leĭkotsitarnykh interferonov, sinteziruemykh bakterial'nymi shtammami-produtsentami.