Recombinant human B cell stimulatory factor 2 (BSF-2/IFN-beta 2) regulates beta-fibrinogen and albumin mRNA levels in Fao-9 cells.
Conditioned medium from human monocytes contains a partially characterized hepatocyte-stimulating factor that simultaneously elevates the mRNA levels of the acute-phase protein beta-fibrinogen and decreases albumin mRNA in rat hepatoma cells. We demonstrate that recombinant human B-cell stimulatory factor 2, which is identical to interferon-beta 2/26 kDa protein and interleukin-HP1, exhibits the same activity as hepatocyte-stimulating factor. Furthermore, a specific antibody against B-cell stimulatory factor 2 was able to inhibit hepatocyte-stimulating factor in conditioned medium from human monocytes. Our data show that hepatocyte-stimulating factor and B-cell stimulatory factor 2 are functionally and immunologically related proteins.
Andus
T
T
Geiger
T
T
Hirano
T
T
Northoff
H
H
Ganter
U
U
Bauer
J
J
Kishimoto
T
T
Heinrich
P C
PC
D000418:Albumins / Q000235:genetics*; D000818:Animals; D002460:Cell Line; D005340:Fibrinogen / Q000235:genetics*; D006801:Humans; D007370:Interferon Type I / Q000494:pharmacology; D015850:Interleukin-6; D008114:Liver Neoplasms, Experimental / Q000378:metabolism*; D008222:Lymphokines / Q000494:pharmacology* / Q000502:physiology; D011506:Proteins / Q000494:pharmacology; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D011994:Recombinant Proteins / Q000494:pharmacology
D000418:Albumins; D007370:Interferon Type I; D015850:Interleukin-6; D008222:Lymphokines; D011506:Proteins; D012333:RNA, Messenger; D011994:Recombinant Proteins; D005340:Fibrinogen