Urotensin I-like immunoreactivity in amacrine cells of the goldfish retina.
Urotensin I-like immunoreactivity (UILI), in different localization from that of corticotropin releasing factor-like immunoreactivity (CRFLI), in the goldfish retina has been demonstrated by means of radioimmunoassay, high-performance liquid chromatography (HPLC) and immunohistochemistry. Radioimmunoassay showed 350 +/- 40 pg/mg prot. of UILI in goldfish retina extracts. The immunoreactive material present in the retina was also characterized by reversed phase HPLC. Some of the UILI co-eluted with synthetic carp UI, though the HPLC experiments suggested the existence of other UILI substance(s) with less hydrophobicity than synthetic UI. By immunohistochemistry, UILI and CRFLI were seen in different amacrine cells of the goldfish retina. It is suggested that UI may be involved in the fish visual transmission system together with CRF and other neuropeptides.
Sakanaka
M
M
McMaster
D
D
Chohan
K
K
Shibasaki
T
T
Stell
W K
WK
Lederis
K
K
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003346:Corticotropin-Releasing Hormone / Q000378:metabolism; D003530:Cyprinidae / Q000378:metabolism*; D006054:Goldfish / Q000378:metabolism*; D007124:Immunoenzyme Techniques; D009474:Neurons / Q000378:metabolism; D010455:Peptides / Q000378:metabolism*; D011863:Radioimmunoassay; D012160:Retina / Q000032:analysis / Q000378:metabolism*; D014579:Urotensins / Q000032:analysis / Q000378:metabolism*
D010455:Peptides; D014579:Urotensins; D003346:Corticotropin-Releasing Hormone; C029676:urotensin I