[Reorganization of the system of intermediate filaments and detection of the organization centers after centrifugation of cells attached to a substrate].
Cultured pig kidney epithelial cells were centrifuged at 20,000 gav so that the centrifugation force was oriented parallel to the substrate, fixed and processed for indirect immunofluorescent staining with tubulin and vimentin antibodies. After a 2 hour centrifugation vimentin filaments aggregated in the centripetal parts of the cells (probably, because of their association with floating lipid vesicles). Microtubule-organizing centers were found near the centripetal poles of the nuclei, which migrated in the direction of the centrifugal force. The distribution of the cytoplasmic microtubules did not change during centrifugation. The staining of the cultures one hour after centrifugation revealed vimentin-containing spots with radiating intermediate filaments in most of the cells. These spots were localized near the cell nuclei; double immunofluorescent staining with tubulin and vimentin antibodies showed that their position was identical to that of the microtubule-organizing centers. Similar foci of vimentin filaments were seen in the cells after a 3-4 hour centrifugation. Probably, these structures participate in organizing the intermediate filament cytoskeleton in cells.
Nadezhdina
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Vaĭsberg
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D000818:Animals; D002458:Cell Fractionation; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000648:ultrastructure*; D004847:Epithelial Cells; D004848:Epithelium / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D007382:Intermediate Filaments / Q000648:ultrastructure*; D007668:Kidney; D008854:Microscopy, Electron; D008870:Microtubules / Q000648:ultrastructure; D013552:Swine; D014461:Ultracentrifugation
Reorganizatsiia sistemy promezhutochnykh filamentov i vyiavlenie tsentrov ikh organizatsii posle tsentrifugirovaniia prikreplennykh k substratu kletok.