Localization and distribution of actin in mammalian sperm heads.
Actin was identified in boar and mole spermatozoa by utilizing indirect immunofluorescence, immunoelectron microscopy, and SDS-PAGE, followed by blot and screening with an anti-actin monoclonal antibody. Actin was detected in two places in the sperm head: the equatorial segment of the acrosome and the postacrosomal region. The protein was present in a nonfilamentous form and was localized under the plasma membrane. A small amount of actin was also detected in the sperm tail. The function of actin in the sperm head is discussed.
Lora-Lamia
C
C
Department of Biology, University of Milan, Italy.
Castellani-Ceresa
L
L
Andreetta
F
F
Cotelli
F
F
Brivio
M
M
D000199:Actins / Q000032:analysis*; D000818:Animals; D005455:Fluorescent Antibody Technique; D007150:Immunohistochemistry; D008297:Male; D008854:Microscopy, Electron; D008970:Molecular Weight; D008971:Moles; D013077:Sperm Head / Q000032:analysis* / Q000648:ultrastructure; D013094:Spermatozoa / Q000032:analysis*; D013552:Swine
D000199:Actins