Effect of betamethasone treatment on luteal lifespan and the LH response to GnRH in dairy cows.
Betamethasone (a synthetic glucocorticoid, 15 mg) was administered i.m. twice daily for 10 days to 4 regularly cycling dairy cows, beginning on Day 10 of the oestrous cycle. Luteal function, monitored by plasma progesterone, was extended by 7, 9, 19 and 20 days, respectively. Luteal function in the next cycle was normal. Endogenous cortisol values were suppressed for 14, 13, 34 and 27 days, respectively. Pituitary responsiveness to 20 micrograms GnRH was assessed by LH measurement on Days -1, +3 and +7 relative to the start of betamethasone treatment. There was a progressive decrease in peak LH concentrations after each GnRH challenge compared to control cows. Hourly measurements of PGF-2 alpha metabolite during the expected period of luteolysis failed to reveal normal increases. It is suggested that betamethasone caused prolonged luteal function, either by directly inhibiting PGF-2 alpha release, or by suppressing pituitary stimulation of follicular growth and hence lowering oestradiol concentrations, since it is known that PGF-2 alpha and oestradiol act synergistically to cause luteolysis.
Dobson
H
H
Alam
M G
MG
Kanchev
L N
LN
D000818:Animals; D001623:Betamethasone / Q000494:pharmacology*; D002417:Cattle / Q000502:physiology*; D003338:Corpus Luteum / Q000187:drug effects / Q000502:physiology*; D015237:Dinoprost; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000494:pharmacology; D006854:Hydrocortisone / Q000097:blood; D007986:Luteinizing Hormone / Q000378:metabolism*; D003341:Luteolysis / Q000187:drug effects*; D010902:Pituitary Gland / Q000187:drug effects / Q000378:metabolism*; D011374:Progesterone / Q000097:blood; D011460:Prostaglandins F / Q000378:metabolism
D011460:Prostaglandins F; D007987:Gonadotropin-Releasing Hormone; D011374:Progesterone; D007986:Luteinizing Hormone; D001623:Betamethasone; D015237:Dinoprost; D006854:Hydrocortisone