Evidence that MAP-2 may be involved in pigment granule transport in squirrel fish erythrophores.
We have demonstrated the presence of MAP-2 in squirrel fish erythrophores using SDS-PAGE, immunoblot, and immunoprecipitation techniques. The monoclonal antibodies used (AP-9, -13, -14) were raised against distinct antigenic sites on Chinese hamster brain MAP-2. Immunoprecipitation studies demonstrated that all three antibodies bind a 300 K protein found in crude cell extracts and in partially purified MAP fractions isolated from erythrophores of the squirrel fish Holocentrus rufus. Immunofluorescent studies confirmed that the 300 K protein was present in cultured erythrophores. Studies of cells induced to aggregate and disperse their pigment granules revealed that the 300 K protein comigrated with the pigment, suggesting that the 300 K protein may constitute part of the "alpha-cytomatrix" involved in pigment translocations.
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D000818:Animals; D001921:Brain / Q000648:ultrastructure; D002856:Chromatophores / Q000502:physiology* / Q000648:ultrastructure; D003594:Cytoplasmic Granules / Q000502:physiology* / Q000648:ultrastructure; D004591:Electrophoresis, Polyacrylamide Gel; D005399:Fishes; D005455:Fluorescent Antibody Technique; D008854:Microscopy, Electron; D008869:Microtubule-Associated Proteins / Q000032:analysis*; D008870:Microtubules / Q000502:physiology* / Q000648:ultrastructure; D008970:Molecular Weight; D010860:Pigments, Biological
D008869:Microtubule-Associated Proteins; D010860:Pigments, Biological