title: A newly established cell line of rabbit lens epithelium.
abstract: Rabbit lens epithelial cells have been cultured continuously for more than 24 months (200 generations) in monolayers. Their morphology resembled cobblestones when confluent and was spindle-shaped during growth. The doubling time was 40 hours. The cells were capable of colony formation and their plating efficiency was about 6%. They had immunoreactivity to antiserum to the crystalline-rich supernatant of rabbit lens homogenate. Therefore, it was concluded that they were a permanent cell line and they were named TOTL-86 cells.
authors.forename: T
authors.initials: T
authors.lastname: Sasabe
authors.forename: K
authors.initials: K
authors.lastname: Kishida
authors.forename: A
authors.initials: A
authors.lastname: Kiritoshi
authors.forename: A
authors.initials: A
authors.lastname: Uni
authors.forename: R
authors.initials: R
authors.lastname: Manabe
mesh_terms: D000818:Animals; D002455:Cell Division; D002460:Cell Line; D003114:Colony-Forming Units Assay; D003584:Cytological Techniques; D004847:Epithelial Cells; D007908:Lens, Crystalline / Q000166:cytology*; D011817:Rabbits; D013997:Time Factors