Studies on in vitro culture supernatant of P. falciparum. Isolation of antigens for serology.
Twenty-four-hour-old in vitro culture supernatant of Plasmodium falciparum was collected and a comparative study was carried out using supernatant antigen and antigen prepared from in vitro culture of P. falciparum parasites in ELISA with sera from slide-positive malaria cases. The results were comparable, and in certain cases higher ELISA readings were observed with the supernatant antigen.
Ray
K
K
Division of Microbiology, National Institute of Communicable Diseases, Delhi, India.
Rao
T S
TS
Sivaraman
C A
CA
Basu
R N
RN
D000818:Animals; D000953:Antigens, Protozoan / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008288:Malaria / Q000276:immunology*; D010963:Plasmodium falciparum / Q000276:immunology*
D000953:Antigens, Protozoan