title: Thermally modified azocasein--a new insoluble substrate for the determination of proteolytic activity.
abstract: An insoluble chromogenic substrate for the determination of proteolytic activity was prepared by heating azocasein in a thin layer at 200 degrees C for 4 h in a hot-air thermostat. The activity of an extracellular bacterial proteinase produced by Bacillus sp. was determined with this new substrate.
authors.affiliation: Laboratory of Analytical Chemistry, Czechoslovak Academy of Sciences, Ceské Budĕjovice.
authors.forename: I
authors.initials: I
authors.lastname: Safarík
mesh_terms: D002364:Caseins; D006358:Hot Temperature; D007700:Kinetics; D010447:Peptide Hydrolases / Q000378:metabolism*; D013053:Spectrophotometry / Q000379:methods
chemical_list: D002364:Caseins; C026018:azocasein; D010447:Peptide Hydrolases