title: Heat shock factor is regulated differently in yeast and HeLa cells.
abstract: When cells are exposed to elevated temperatures, transcription of a small set of genes, the heat-shock genes, is activated. This response is mediated by a short DNA sequence, the heat-shock element (HSE), which is thought to be the binding site for a specific transcription factor. Studies with Drosophila show that this protein binds to HSEs only in heat-shocked cells, suggesting that changes in factor binding are responsible for gene activation. We have investigated the properties of HSE-binding proteins from yeast and HeLa cells. In HeLa cells, binding activity is present only after heat shock. In contrast, control and heat-shocked yeast cells yield the same amount of HSE-binding activity; however, the mobility of protein-HSE complexes on polyacrylamide gels is altered following heat shock. This mobility difference can be significantly reduced by treatment of crude extracts with phosphatase. We propose that the yeast heat-shock factor binds constitutively to DNA but only activates transcription after heat-induced phosphorylation.
authors.forename: P K
authors.initials: PK
authors.lastname: Sorger
authors.forename: M J
authors.initials: MJ
authors.lastname: Lewis
authors.forename: H R
authors.initials: HR
authors.lastname: Pelham
mesh_terms: D005786:Gene Expression Regulation; D005796:Genes; D006367:HeLa Cells / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000235:genetics*; D006358:Hot Temperature; D006801:Humans; D007700:Kinetics; D046911:Macromolecular Substances; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D015533:Transcriptional Activation
chemical_list: D006360:Heat-Shock Proteins; D046911:Macromolecular Substances