Requirement of a heat-labile factor(s) for in vitro expression of the amp gene of pBR322.
The amp gene of pBR328 and pBR322 was expressed less efficiently at 45 than at 30 degrees C in an in vitro coupled transcription-translation system of Escherichia coli. Preincubation of S30 extract at 45 degrees C reduced specifically its ability to express the amp gene at 30 degrees C, indicating inactivation of a factor(s) required for efficient expression of the amp gene.
Kuriki
Y
Y
Institute for Protein Research, Osaka University, Japan.
D000123:Acetyltransferases / Q000096:biosynthesis / Q000235:genetics; D015500:Chloramphenicol O-Acetyltransferase; D004792:Enzyme Precursors / Q000096:biosynthesis / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005786:Gene Expression Regulation; D005798:Genes, Bacterial; D014176:Protein Biosynthesis; D013696:Temperature; D014158:Transcription, Genetic; D001618:beta-Lactamases / Q000096:biosynthesis / Q000235:genetics*
D004792:Enzyme Precursors; D000123:Acetyltransferases; D015500:Chloramphenicol O-Acetyltransferase; D001618:beta-Lactamases