title: Studies on interaction of 5 S RNA with ribosomal proteins.
abstract: Proteins of the large ribosomal subunit of rat liver (TP 60) were immobilized by diffusion transfer onto nitrocellulose after two-dimensional polyacrylamide gel electrophoresis (2-D PAGE). Incubation of the TP 60 blots with 32P-labeled 5 S RNA under defined ionic conditions (300 mM KCl, 20 mM MgCl2) resulted in specific binding to a limited set of ribosomal proteins consisting of proteins L3, L4, L6, L13/15 and--to a lesser extent--L7 and L19. Under identical conditions, blots with proteins of the small ribosomal subunit (TP 40) did not bind 5 S RNA.
authors.forename: H J
authors.initials: HJ
authors.lastname: Kärgel
authors.forename: J
authors.initials: J
authors.lastname: Stahl
authors.forename: B
authors.initials: B
authors.lastname: Gross
authors.forename: S
authors.initials: S
authors.lastname: Knespel
authors.forename: H
authors.initials: H
authors.lastname: Bielka
authors.forename: M
authors.initials: M
authors.lastname: Saarma
mesh_terms: D002352:Carrier Proteins / Q000378:metabolism*; D003101:Collodion; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000032:analysis; D012335:RNA, Ribosomal / Q000378:metabolism*; D016601:RNA-Binding Proteins; D012269:Ribosomal Proteins / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000032:analysis
chemical_list: D002352:Carrier Proteins; D012335:RNA, Ribosomal; D016601:RNA-Binding Proteins; D012269:Ribosomal Proteins; C029609:ribosomal protein L18; D003101:Collodion