title: [Immunoenzyme method of sequential saturation for determining antigens using the model of insulin].
abstract: The method for the determination of insulin by means of the enzyme immunoassay, based on the use of insulin-peroxidase conjugates, has been developed. In this assay the scheme of the successive saturation of the active sites of antibodies is used. The antigenic properties of two conjugates differing in the method of their preparation are compared. The conjugates were obtained by the covalent binding of peroxidase, oxidized in its carbohydrate component, with insulin (conjugate 1) or hexamethylene-diamine-modified insulin (conjugate 2). The conjugates represented a mixture of oligomers differing in their molecular weight. Conjugate 1 possessed higher affinity to antibodies and higher enzymatic activity than conjugate 2. The method for evaluating the quality of antisera to insulin used in the assay has been proposed. The time of the insulin assay is 5-16 hours, the limit of insulin detection is 5 microU/ml, the variation factor is 3-12%.
authors.forename: E B
authors.initials: EB
authors.lastname: Dikova
authors.forename: N V
authors.initials: NV
authors.lastname: Sorokina
authors.forename: E M
authors.initials: EM
authors.lastname: Gavrilova
authors.forename: A M
authors.initials: AM
authors.lastname: Egorov
mesh_terms: D000327:Adsorption; D000818:Animals; D000918:Antibody Specificity; D000941:Antigens / Q000032:analysis*; D001666:Binding Sites, Antibody; D005069:Evaluation Studies as Topic; D006168:Guinea Pigs; D007124:Immunoenzyme Techniques / Q000295:instrumentation; D007202:Indicators and Reagents; D007328:Insulin; D007330:Insulin Antibodies / Q000032:analysis*; D013997:Time Factors
chemical_list: D000941:Antigens; D007202:Indicators and Reagents; D007328:Insulin; D007330:Insulin Antibodies
vernacular_title: Immunofermentnyĭ metod posledovatel'nogo nasyshcheniia dlia opredeleniia antigenov na modeli insulina.