title: The conformation of T4 bacteriophage dihydrofolate reductase from circular dichroism.
abstract: The secondary and tertiary structure of T4 bacteriophage dihydrofolate reductase is investigated by vacuum ultraviolet circular dichroism (CD) spectroscopy and probability analysis of the primary amino acid sequence. The far ultraviolet CD spectrum of the enzyme in the range of 260-178 nm is analyzed by the generalized inverse and variable selection methods developed by our laboratory. Variable selection yields an average content of 26% alpha-helix, 21% antiparallel beta-sheet, 10% parallel beta-sheet, 20% beta-turns, and 32% "other" structures within the T4 protein. The characteristic peaks of the CD spectrum indicate that the enzyme has a lot of antiparallel beta-sheet, which is typical of the alpha + beta tertiary class of globular proteins. The secondary structure of the protein is also analyzed by using four statistical methods on the amino acid sequence. Although the secondary structures predicted by each individual statistical method vary to a considerable extent, the fractions of each structure jointly predicted by a majority of the methods are in excellent agreement with our CD analysis. The alternating arrangement for some segments of alpha-helix and beta-sheet predicted from primary structure to be within the enzyme is characteristic of proteins containing parallel beta-sheet. This supports our conclusion that the protein contains both parallel and antiparallel beta-sheet structures, but finding both types of beta-sheet also means that the protein may have the variation on alpha/beta tertiary structure recently found in EcoRI endonuclease and thymidylate synthase. These observations, in conjunction with other physical properties of the T4 reductase, suggest that the enzyme perhaps shares an evolution in common with the dihydrofolate reductases derived from type I R-plasmids rather than with the host-cell protein.
authors.affiliation: Department of Biochemistry and Biophysics, Oregon State University, Corvallis 97331.
authors.forename: L A
authors.initials: LA
authors.lastname: Compton
authors.forename: C K
authors.initials: CK
authors.lastname: Mathews
authors.forename: W C
authors.initials: WC
authors.lastname: Johnson
mesh_terms: D002942:Circular Dichroism; D004926:Escherichia coli / Q000201:enzymology*; D046911:Macromolecular Substances; D011487:Protein Conformation; D013056:Spectrophotometry, Ultraviolet; D013604:T-Phages / Q000201:enzymology*; D013762:Tetrahydrofolate Dehydrogenase
chemical_list: D046911:Macromolecular Substances; D013762:Tetrahydrofolate Dehydrogenase