[Biological responses, ultrastructural changes and metabolism of estrone sulfate in line cells of human breast cancer: MCF-7].
The biological responses, the metabolism and the ultrastructural modifications provoked by estrone sulfate in the MCF-7 mammary cancer cell line are studied. The data indicate: 1) A fraction of estrone sulfate is hydrolyzed and the freed hormone is sufficient to elicit a biological response (progesterone receptor). 2) Using [3H]estrone sulfate, 24 h after culture, it is observed that the great part of the radioactive material in the cells is in the form of unconjugated estrogens. 3) The main transformation product of [3H]estrone sulfate is estradiol. This is found particularly in the nucleus. 4) Tamoxifen inhibits very significantly the conversion of estrone sulfate into estradiol. 5) Electronmicroscopic observations indicate that the estrone sulfate provokes an important development in the secretory system. In conclusion, it is suggested that the estrone sulfate can play an important role in the response to estrogens in human mammary cancer.
Gelly
C
C
Equipe de Recherche du CNRS UA 548, Fondation de Recherche en Hormonologie, Paris, France.
Lecerf
F
F
Pasqualini
J R
JR
D001943:Breast Neoplasms / Q000378:metabolism / Q000648:ultrastructure; D002460:Cell Line; D004970:Estrone / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D005260:Female; D006801:Humans; D006868:Hydrolysis; D011980:Receptors, Progesterone / Q000378:metabolism; D013629:Tamoxifen / Q000494:pharmacology
D011980:Receptors, Progesterone; D013629:Tamoxifen; D004970:Estrone; C017296:estrone sulfate
Réponses biologiques, modifications ultrastructurales et métabolisme du sulfate d'oestrone dans la lignée cellularire de cancer mammaire humain: MCF-7.