Glycerol increases the cytochemical detectability of cholesterol in the apical plasma membrane of uterine epithelial cells.
The apical plasma membrane of uterine epithelial cells in the rat has been treated with glycerol before fixation and then examined by freeze-fracture cytochemistry using digitonin and filipin. Many more lesions were produced by both cytochemicals following glycerol treatment than in untreated controls, and we suggest that this indicates an increased detectability of cholesterol. We consider the implications of the findings for the way in which glycerol acts on membranes and propose that glycerol promotes increased binding between cholesterol and the cytochemicals.
Murphy
C R
CR
Dwarte
D M
DM
D000818:Animals; D002462:Cell Membrane / Q000378:metabolism*; D002784:Cholesterol / Q000378:metabolism*; D004072:Digitonin; D004848:Epithelium / Q000648:ultrastructure; D005260:Female; D005372:Filipin; D005614:Freeze Fracturing; D005990:Glycerol; D006652:Histological Techniques; D008563:Membrane Lipids / Q000378:metabolism*; D051381:Rats; D014599:Uterus / Q000187:drug effects / Q000648:ultrastructure*
D008563:Membrane Lipids; D005372:Filipin; D002784:Cholesterol; D004072:Digitonin; D005990:Glycerol