title: Synthesis and secretion of wheat alpha-amylase in Saccharomyces cerevisiae.
abstract: A wheat alpha-amylase cDNA clone has been fused to the phosphoglycerate kinase initiator methionine to enable synthesis in the yeast Saccharomyces cerevisiae of an alpha-amylase enzyme that is identical in size to the wild-type alpha-amylase. The alpha-amylase is synthesized with an N-terminal plant signal peptide which is recognized in the yeast host, leading to efficient processing and secretion into the medium. The secretion of alpha-amylase into the medium is quite efficient in rich medium, but barely detectable in a minimal medium.
authors.affiliation: Plant Breeding Institute, Trumpington, Cambridge, U.K.
authors.forename: S J
authors.initials: SJ
authors.lastname: Rothstein
authors.forename: K N
authors.initials: KN
authors.lastname: Lahners
authors.forename: C M
authors.initials: CM
authors.lastname: Lazarus
authors.forename: D C
authors.initials: DC
authors.lastname: Baulcombe
authors.forename: A A
authors.initials: AA
authors.lastname: Gatenby
mesh_terms: D003470:Culture Media / Q000032:analysis; D010940:Plant Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D021382:Protein Sorting Signals / Q000235:genetics / Q000502:physiology; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis*; D012441:Saccharomyces cerevisiae / Q000235:genetics; D014908:Triticum / Q000201:enzymology / Q000235:genetics*; D000516:alpha-Amylases / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism
chemical_list: D003470:Culture Media; D010940:Plant Proteins; D021382:Protein Sorting Signals; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D000516:alpha-Amylases