Induction of mouse monocyte-macrophage replication by a mesangial cell-derived factor.
Conditioned media of cultured mouse mesangial cells (possessing microfilaments) were shown to contain a factor that stimulated splenic monocytes-macrophages and blood monocytes to replicate. Replicated cells were shown to express MAC-1 antigen as demonstrated by immunofluorescence with anti-MAC 1 and to possess Fc receptors as evidenced by their capacity to ingest sensitized erythrocytes. Preliminary characterization revealed the following characteristics: by Amicon ultrafiltration, fractions greater than 100,000 daltons were shown to have biologic activity; chromatofocusing of these active fractions revealed a peak of activity associated with fractions having pH 4; heating to 100 degrees C for 10 minutes abolished almost all activity, whereas trypsin treatment was without effect. The observations suggest a mechanism by which mesangial cells may modulate the proliferation of monocytes-macrophages that infiltrate the glomerulus in glomerulonephritis.
Ooi
B S
BS
Department of Internal Medicine, University of Cincinnati College of Medicine, OH.
MacCarthy
E P
EP
Weiss
M A
MA
Hsu
A
A
Ooi
Y M
YM
D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D002455:Cell Division; D002457:Cell Extracts / Q000494:pharmacology*; D002478:Cells, Cultured; D005920:Glomerular Mesangium / Q000166:cytology*; D006358:Hot Temperature; D016177:Macrophage-1 Antigen; D008264:Macrophages / Q000166:cytology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009000:Monocytes / Q000166:cytology*; D010587:Phagocytosis; D013936:Thymidine / Q000378:metabolism; D014020:Tissue Extracts / Q000494:pharmacology*; D014357:Trypsin / Q000378:metabolism
D000954:Antigens, Surface; D002457:Cell Extracts; D016177:Macrophage-1 Antigen; D014020:Tissue Extracts; D014357:Trypsin; D013936:Thymidine