Microbial protein and peptide metabolism in rumen fluid from faunated and ciliate-free sheep.
1. Protease and deaminase activities and the metabolism of peptides were measured in rumen fluid from ciliate-free sheep and from sheep with a limited population of small entodinia. The same measurements were repeated following inoculation of the latter group with a more typical mixed ciliate population. 2. Protease and dialanine uptake activities of mixed rumen micro-organisms were not significantly influenced by protozoa. Trialanine uptake, leucine aminopeptidase (EC 3.4.11.1), deaminase and trypsin-like protease activities were 70, 107, 73 and 91% higher with the limited population, and 72, 58, 64 and 55% higher when mixed protozoa were present, indicating a major role for the protozoa in these activities.
Wallace
R J
RJ
Broderick
G A
GA
Brammall
M L
ML
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D003641:Deamination; D004032:Diet; D056890:Eukaryota / Q000378:metabolism*; D005750:Gastric Juice / Q000378:metabolism*; D010447:Peptide Hydrolases / Q000378:metabolism; D010455:Peptides / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism*; D012417:Rumen / Q000378:metabolism*; D012756:Sheep
D000596:Amino Acids; D010455:Peptides; D011506:Proteins; D010447:Peptide Hydrolases