Static bend of DNA helix at the activator recognition site of the ompF promoter in Escherichia coli.
Expression of the ompF gene coding for a major outer membrane protein of Escherichia coli is regulated by osmolarity of the medium. The ompF expression is controlled by a transcriptional activation mechanism which requires the ompR gene product acting on a region located upstream from the canonical -35 and -10 regions of the ompF gene. Evidence is presented that the upstream region of the ompF promoter contains a static bend of DNA having two sets of oligo (dA X dT) tracts with periodical spacing. It was demonstrated that the bending region overlaps with the recognition site for the activator protein, OmpR.
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D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D009690:Nucleic Acid Conformation; D011401:Promoter Regions, Genetic; D012091:Repetitive Sequences, Nucleic Acid
D001425:Bacterial Outer Membrane Proteins; D004269:DNA, Bacterial