Two-level factorial screening for influence of temperature, pH, and aeration on production of Serratia marcescens nuclease.
Two high-nuclease-yielding mutants of Serratia marcescens, derived by chemical mutagenesis (W280, W355), and two strains with the pBR322 plasmid 403-SD2, carrying a nuclease gene and a chloramphenicol resistance gene [Escherichia coli CSH50(403-SD2) and S. marcescens CH30(403-SD2)] were investigated for nuclease production in a factorial shake flask experiment, with temperature (30 and 37 degrees C), pH (with or without CaCO3 tablets), and aeration (with or without baffles) as variable conditions. Yields varied 10-fold depending on the conditions investigated.
Jepsen
P K
PK
Department of Biotechnology, Technical University of Denmark, Lyngby.
Riise
E
E
Biedermann
K
K
Kristensen
P C
PC
Emborg
C
C
D000332:Aerobiosis; D003851:Deoxyribonucleases / Q000096:biosynthesis*; D005798:Genes, Bacterial; D006863:Hydrogen-Ion Concentration; D009154:Mutation; D012706:Serratia marcescens / Q000201:enzymology* / Q000235:genetics; D013696:Temperature
D003851:Deoxyribonucleases