Multiple forms of biologically active insulin-like material from mouse fetal brain cells in culture.
The biological and immunological properties of insulin-like material produced by brain cells from 12 day old mice, cultured in serum-free media were examined. Differences were seen in the rate of appearance, relative amounts and behaviour on HPLC between immunoreactive and biologically active insulin-like material. Excess anti-insulin sera failed to suppress most of the biological activity and the culture medium was shown to contain relatively large quantities of immunoreactive IGF-I (590 ng/30 ml). The elution profile of this material on HPLC overlapped but was not identical with the insulin biological activity, which indicated that other insulin-like growth factors were also present. The relatively high concentration of IGF-I produced by the brain cell cultures suggest that this preparation may be suitable for biosynthetic studies of this growth factor.
Birch
N P
NP
Christie
D L
DL
Renwick
A G
AG
D000818:Animals; D001681:Biological Assay; D001921:Brain / Q000166:cytology / Q000196:embryology / Q000378:metabolism*; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D005333:Fetus / Q000378:metabolism*; D007328:Insulin / Q000378:metabolism*; D007334:Insulin-Like Growth Factor I / Q000378:metabolism; D051379:Mice; D011863:Radioimmunoassay
D007328:Insulin; D007334:Insulin-Like Growth Factor I