title: Antigen density on the surface of mononuclear cells in SLE.
abstract: Searching for the cause of the known immunological abnormalities in systemic lupus erythematosus (SLE), the density of cell surface antigens was measured after immunofluorescent staining in a cell sorter. The densities of CD3, CD4, CD5, CD8 and sIgM lymphocyte antigens were the same on patients' lymphocytes as on lymphocytes from healthy subjects. The intensity of HLA-DR immunofluorescence was found to be decreased on patients' monocytes, while the expression of HLA-DR on lymphocytes of patients with SLE hardly differed from that in healthy subjects. Pretreatment of normal mononuclear cells with patients' sera free from immune complexes decreased the binding of anti-HLA-DR antibody to normal monocytes, but it hardly caused alteration on lymphocytes. After culturing, the expression of HLA-DR antigen on patients' monocytes became the same as on normal cells. A causal role of anti-HLA-DR autoantibodies is suggested and discussed.
authors.affiliation: 3rd Department of Medicine, University Medical School of Debrecen, Hungary.
authors.forename: G
authors.initials: G
authors.lastname: Szegedi
authors.forename: P
authors.initials: P
authors.lastname: Surányi
authors.forename: S
authors.initials: S
authors.lastname: Damjanovich
mesh_terms: D000943:Antigens, Differentiation / Q000032:analysis; D000954:Antigens, Surface / Q000032:analysis*; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006680:HLA Antigens / Q000032:analysis; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D011947:Receptors, Antigen, B-Cell / Q000032:analysis
chemical_list: D000943:Antigens, Differentiation; D000954:Antigens, Surface; D006680:HLA Antigens; D011947:Receptors, Antigen, B-Cell