title: Monoclonal antibodies to and immunoaffinity purification of choline acetyltransferase from bovine brain.
abstract: Three hybridomas producing monoclonal antibodies to bovine brain choline acetyltransferase (ChAT) have been established by fusion of the spleen cells from a mouse immunized with purified enzyme with myeloma NS-1 cells. All three clones produced IgGl antibodies that reacted with enzyme protein denatured with sodium dodecyl sulfate. By using one of the monoclonal antibodies, a rapid and efficient immunoaffinity purification procedure of bovine ChAT has been established. Immunoblot analysis and immunoaffinity purification indicated that bovine ChAT is a single 68-kilodalton protein. The monoclonal antibodies will offer us a good tool to isolate the cDNA clones encoding ChAT.
authors.forename: I
authors.initials: I
authors.lastname: Ishida
authors.forename: T
authors.initials: T
authors.lastname: Ichikawa
authors.forename: T
authors.initials: T
authors.lastname: Deguchi
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D001921:Brain / Q000201:enzymology*; D002417:Cattle; D002795:Choline O-Acetyltransferase / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D007163:Immunosorbent Techniques; D008970:Molecular Weight
chemical_list: D000911:Antibodies, Monoclonal; D002795:Choline O-Acetyltransferase