Chromosomal analysis of bone marrow stromal fibroblasts in allogeneic HLA compatible sibling bone marrow transplantations.
Karyotyping was performed on bone marrow stromal fibroblasts and marrow haemopoietic cells on six patients who received bone marrow transplants from siblings of the opposite sex. Three patients with severe aplastic anaemia received unmanipulated donor bone marrow cells. Three other patients with leukaemia and conditioned with high dose chemotherapy (+ total body irradiation in two patients) received T-cell depleted marrow mononuclear cells. Marrow chromosomal analysis was performed on samples obtained between 6 weeks and 1 yr post-transplant. All marrow fibroblasts studied were of recipient origin, whereas all haemopoietic cells were of donor origin. These results demonstrate that in allogeneic sibling marrow transplantation, recipient bone marrow stromal cells regenerate and repopulate the bone marrow and this is not influenced by the conditioning regimen used, the type and dose of marrow cells given and the pre-existing disease.
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Purvis-Smith
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Biggs
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D000741:Anemia, Aplastic / Q000473:pathology* / Q000628:therapy; D001853:Bone Marrow / Q000473:pathology; D016026:Bone Marrow Transplantation; D003131:Combined Modality Therapy; D005260:Female; D005347:Fibroblasts / Q000637:transplantation* / Q000648:ultrastructure; D006085:Graft Survival; D006412:Hematopoietic Stem Cells / Q000648:ultrastructure; D006648:Histocompatibility; D006801:Humans; D007938:Leukemia / Q000473:pathology / Q000628:therapy; D008297:Male; D012801:Sibling Relations; D014184:Transplantation, Homologous