Polyethylene glycol enzyme immunoassay for screening anti-haptoglobin monoclonal antibodies.
An enzyme immunoassay for the screening of anti-haptoglobin activities in supernatants of hybridoma cell cultures is described. The sandwich technique was greatly improved by the addition of 2% polyethylene glycol 6000 to all the reagents. It allowed a six-fold shortening in the incubation time compared with the standard method without sacrificing either sensitivity or accuracy.
Katnik
I
I
Department of Biochemistry, Faculty of Pharmacy, Medical Academy, Wrocław, Poland.
Podgórska
M
M
Dobryszycka
W
W
D000911:Antibodies, Monoclonal / Q000032:analysis*; D000936:Antigen-Antibody Complex; D004306:Dose-Response Relationship, Immunologic; D006242:Haptoglobins / Q000276:immunology*; D007124:Immunoenzyme Techniques; D011092:Polyethylene Glycols; D011485:Protein Binding; D012995:Solubility
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D006242:Haptoglobins; D011092:Polyethylene Glycols