title: An in vitro slice preparation of the cat lateral geniculate nucleus.
abstract: A slice preparation of the cat thalamus containing the lateral geniculate nucleus and the terminal portion of the optic tract is described. Ultrastructurally the slices remain relatively normal for only a short time after cutting. Indeed most cellular elements deteriorate quickly with time but patches of relatively intact tissue were still present even 10 h after cutting and maintenance in a storage bath. However, for 4-5 h after cutting long-lasting intracellular recordings of high quality and stability were obtained, and intrasomatic injection of horseradish peroxidase used for the morphological identification of recorded neurones as X or Y cells.
authors.forename: V
authors.initials: V
authors.lastname: Crunelli
authors.forename: N
authors.initials: N
authors.lastname: Leresche
authors.forename: J W
authors.initials: JW
authors.lastname: Hynd
authors.forename: N M
authors.initials: NM
authors.lastname: Patel
authors.forename: J G
authors.initials: JG
authors.lastname: Parnavelas
mesh_terms: D000818:Animals; D002415:Cats; D004594:Electrophysiology; D005829:Geniculate Bodies / Q000502:physiology* / Q000648:ultrastructure; D006652:Histological Techniques; D006735:Horseradish Peroxidase; D066298:In Vitro Techniques; D008839:Microelectrodes; D008854:Microscopy, Electron; D013569:Synapses / Q000648:ultrastructure
chemical_list: D006735:Horseradish Peroxidase