The effects of oxygen free radicals on the preserved kidney.
This study evaluated the effect of specific scavengers of oxygen derived free radicals on the results of kidney preservation. The immediate function of rabbit kidneys preserved for 24 hr by hypothermic perfusion was studied on an ex vivo shunt. A significant improvement in creatinine clearance was seen when the perfusate was treated with superoxide dismutase (SOD) and catalase (CAT), with values of 261 +/- 82 ml/hr vs control values of 203 +/- 72 ml/hr, P less than 0.05. This effect was enhanced if a long-persistent polyethylene glycol-linked form of SOD, namely PEG-SOD, was used (330 +/- 58 ml/hr, P less than 0.01). Recipient treatment and other modifications designed to protect against free radicals resulted in similar improvement in function. In contrast, no effect of free radical scavengers could be demonstrated in kidneys which were preserved by flush cooling, whether the agents were added to the flushing solution, given to the recipient, or both.
Bennett
J F
JF
Bry
W I
WI
Collins
G M
GM
Halasz
N A
NA
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002374:Catalase / Q000494:pharmacology*; D003080:Cold Temperature; D003404:Creatinine / Q000378:metabolism; D005609:Free Radicals; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism; D016030:Kidney Transplantation; D009926:Organ Preservation; D010100:Oxygen / Q000494:pharmacology*; D010477:Perfusion; D011092:Polyethylene Glycols / Q000008:administration & dosage; D011817:Rabbits; D013482:Superoxide Dismutase / Q000008:administration & dosage / Q000494:pharmacology*
D000975:Antioxidants; D005609:Free Radicals; D011092:Polyethylene Glycols; D003404:Creatinine; D002374:Catalase; D013482:Superoxide Dismutase; D010100:Oxygen