Complementary DNA for human T-cell cyclophilin.
Complementary DNA encoding human cyclophilin, a specific cyclosporin A-binding protein, has been isolated from the leukemic T-cell line Jurkat and sequenced. Comparison of the deduced amino acid sequence with the previously determined sequence of bovine thymus cyclophilin reveals only three differences: an additional amino acid at the carboxy terminus end and two internal changes. RNA transfer blot analysis indicates an mRNA size of approximately 1 kb for human T-cell cyclophilin. Phytohaemagglutinin and phorbol myristate acetate induction of T cells treated or not with cyclosporin A affects only marginally the level of cyclophilin mRNA. Southern blot analysis of human genomic DNA digested with different restriction enzymes strongly suggests the existence of a multigene family for cyclophilin.
Haendler
B
B
Hofer-Warbinek
R
R
Hofer
E
E
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D002460:Cell Line; D003001:Cloning, Molecular; D003524:Cyclosporins / Q000378:metabolism; D004247:DNA / Q000302:isolation & purification*; D005796:Genes; D006801:Humans; D009693:Nucleic Acid Hybridization; D019696:Peptidylprolyl Isomerase; D013601:T-Lymphocytes / Q000378:metabolism*
D002352:Carrier Proteins; D003524:Cyclosporins; D004247:DNA; D019696:Peptidylprolyl Isomerase