Peroxidative activation of 3,3'-dichlorobenzidine to mutagenic products in the Salmonella typhimurium test.
The direct and H2O2-dependent mutagenicity of 3,3'-dichlorobenzidine (DCB) were compared in Salmonella tester strains TA98, TA98/1,8-DNP6, TA100 and TA102 using the Ames test. DCB exhibited both direct and H2O2-dependent mutagenicity to both tester strains TA98 and TA98/1,8-DNP6. This H2O2-dependent mutagenicity of DCB was prevented by horseradish peroxidase. DCB, in contrast to its effects in tester strains TA98, was not mutagenic to TA100 and TA102 either directly or in the presence of H2O2. These results suggest that mechanisms, perhaps enzymes endogenous to tester strains TA98, may play a role in the activation of DCB.
Lang
B
B
Iba
M M
MM
D015101:3,3'-Dichlorobenzidine / Q000378:metabolism* / Q000633:toxicity; D001560:Benzidines / Q000378:metabolism* / Q000633:toxicity; D001711:Biotransformation; D006735:Horseradish Peroxidase / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000633:toxicity*; D009152:Mutagenicity Tests; D009153:Mutagens / Q000378:metabolism*; D012486:Salmonella typhimurium / Q000187:drug effects
D001560:Benzidines; D009153:Mutagens; D015101:3,3'-Dichlorobenzidine; C029876:benzidine; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase