title: An improved immunoblotting procedure for the detection of antibodies against HIV.
abstract: Immunoblotting ('Western blotting') is routinely used for detection of antibodies against HIV in the diagnosis of HIV infection. We describe an improved procedure, which does not require virus purification and is easy to control for 'false-positive' results. The technique also does not produce erroneous results due to reactivity of the developing system with residual cellular proteins or viral antigens and does not give high nonspecific background staining. The technique can be applied to the detection of antibodies to HIV in serum, plasma, and blood products.
authors.forename: R
authors.initials: R
authors.lastname: Thorpe
authors.forename: M D
authors.initials: MD
authors.lastname: Brasher
authors.forename: C R
authors.initials: CR
authors.lastname: Bird
authors.forename: A J
authors.initials: AJ
authors.lastname: Garrett
authors.forename: J P
authors.initials: JP
authors.lastname: Jacobs
authors.forename: P D
authors.initials: PD
authors.lastname: Minor
authors.forename: G C
authors.initials: GC
authors.lastname: Schild
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000175:diagnosis; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral / Q000032:analysis*; D000936:Antigen-Antibody Complex / Q000032:analysis; D006678:HIV / Q000276:immunology*; D006801:Humans; D007163:Immunosorbent Techniques
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000936:Antigen-Antibody Complex