Accurate initiation at RNA polymerase II promoters in extracts from Saccharomyces cerevisiae.
A yeast nuclear extract supported transcription from the CYC1 and PYK1 promoters. Transcription was initiated in vitro at or near sites used in vivo. Deletion of "TATA" sequences abolished the reaction. alpha-Amanitin (10 micrograms/ml) and chloride (100 mM) were highly inhibitory.
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Department of Cell Biology, Stanford University School of Medicine, CA 94305.
Kornberg
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D000085:Acetates; D002474:Cell-Free System; D003001:Cloning, Molecular; D005800:Genes, Fungal; D011401:Promoter Regions, Genetic; D012319:RNA Polymerase II / Q000378:metabolism*; D012045:Regulatory Sequences, Nucleic Acid; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D013698:Templates, Genetic; D014157:Transcription Factors / Q000378:metabolism; D014158:Transcription, Genetic
D000085:Acetates; D014157:Transcription Factors; D012319:RNA Polymerase II