Sequences implicated in the processing of Thermus thermophilus HB8 23S rRNA.
Nuclease S1 mapping analyses were performed in order to detect processing intermediates of pre-23S rRNA from Thermus thermophilus HB8. Two processing sites were identified downstream the start of transcription and several consecutive cleavage sites are associated with the mature 5'-end. In the 3'-flanking region one "primary" site and two cleavages which generate short-living intermediates were detected. A series of successive intermediates in the region of the mature 3'-end implies the existence of--in analogy to Escherichia coli--a 3'-exonucleolytic activity. The data were correlated with potential secondary structures within the pre-23S rRNA, which exhibit various repeated sequence elements. M13 sequencing data support the existence of one secondary structural element associated with the strong "primary" cleavage site in the 3'-flanking region. In T. thermophilus we can exclude the formation of an extended base-paired and precursor-specific stem enclosing the 23S rRNA which is inferred to mediate recognition by RNase III in E. coli.
Hartmann
R K
RK
Freie Universität Berlin, Institut für Biochemie, FRG.
Ulbrich
N
N
Erdmann
V A
VA
D001483:Base Sequence; D004722:Endoribonucleases / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D029968:Escherichia coli Proteins; D005796:Genes; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012322:RNA Precursors / Q000378:metabolism; D012323:RNA Processing, Post-Transcriptional; D012335:RNA, Ribosomal / Q000096:biosynthesis*; D012338:RNA, Ribosomal, 23S / Q000096:biosynthesis* / Q000235:genetics; D043244:Ribonuclease III; D013824:Thermus / Q000235:genetics* / Q000378:metabolism
D029968:Escherichia coli Proteins; D012322:RNA Precursors; D012335:RNA, Ribosomal; D012338:RNA, Ribosomal, 23S; D004722:Endoribonucleases; D043244:Ribonuclease III; C468366:ribonuclease III, E coli