Determination of polyamines and metabolites in cerebrospinal fluid by isotope dilution mass fragmentography, and a clinical application.
Capillary gas chromatography and mass fragmentography was used to determine simultaneously 1,3-diaminopropane, putrescine, cadaverine, spermidine, spermine, isoputreanine and putreanine in cerebrospinal fluid. After addition of deuterium labelled analogs and acid hydrolysis, the compounds were isolated by adsorption onto silica and converted into their N-heptafluorobutyryl-methylesters. Quality control data and an application of the method are given. A patient harbouring an astrocytoma was monitored during chemotherapeutic treatment.
van den Berg
G A
GA
Central Laboratory for Clinical Chemistry, University Hospital, Groningen, The Netherlands.
Schaaf
J M
JM
Nagel
G T
GT
Teelken
A W
AW
Muskiet
F A
FA
D001254:Astrocytoma / Q000134:cerebrospinal fluid; D001932:Brain Neoplasms / Q000134:cerebrospinal fluid; D002849:Chromatography, Gas; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007201:Indicator Dilution Techniques; D011073:Polyamines / Q000134:cerebrospinal fluid*
D011073:Polyamines