title: New detection method for uropepsinogen (PGA) using isoelectric focusing and immunoblotting techniques.
abstract: Uropepsinogen (PGA) was isolated and purified from human urine using a column chromatography series. The purified PGA was injected into a rabbit and a PGA-specific antibody was obtained. PGA isozymogen in human urine could be detected reproducibly by immunoblotting using this antibody after isoelectric focusing electrophoresis (IEF) on polyacrylamide gels. This technique may prove to be useful in the genetic study of PGA polymorphism.
authors.forename: T
authors.initials: T
authors.lastname: Yasuda
authors.forename: Y
authors.initials: Y
authors.lastname: Ikehara
authors.forename: W
authors.initials: W
authors.lastname: Sato
authors.forename: K
authors.initials: K
authors.lastname: Kishi
mesh_terms: D002648:Child; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007158:Immunologic Techniques; D007525:Isoelectric Focusing; D008297:Male; D020116:Pepsinogen A; D010435:Pepsinogens / Q000235:genetics / Q000652:urine*; D011110:Polymorphism, Genetic
chemical_list: D010435:Pepsinogens; D020116:Pepsinogen A