[Formation and properties of artificial polycistrons containing truncated genes for E. coli tryptophan operon and phage M13 envelope protein].
Using gene fragments encoding the leader peptide of E. coli tryptophane operon (as duplicated fragment HhaI-140) or M13 phage coat protein (as TaqI-381 or HaeIII-1623 fragments) and basing on pDS1 family of plasmids, expression vectors have been constructed which contained transcription promoters Ptrp, PVIII, and Pv + PVIII, respectively. An artificial gene for human leukocyte interferon alpha 2 (ifn-alpha 2) has been cloned into these plasmids, so that its transcription was a part of polycistronic mRNA and preceding translation was terminated upstream to the ribosome binding site and starting codon of the interferon gene. E. coli cells harbouring these recombinant plasmids provided high level of the interferon biosynthesis. The effect of the mRNA length on the amount of protein synthesised under control of the M13 coat protein transcription-translation signals has been found.
Kravchenko
V V
VV
Gileva
I P
IP
Shamin
V V
VV
Kulichkov
V A
VA
Dobrynin
V N
VN
D001483:Base Sequence; D003090:Coliphages / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005786:Gene Expression Regulation; D005796:Genes; D005798:Genes, Bacterial; D005813:Genes, Synthetic; D005814:Genes, Viral; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D009876:Operon; D010957:Plasmids; D021382:Protein Sorting Signals / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D011995:Recombination, Genetic; D014364:Tryptophan / Q000235:genetics; D014759:Viral Envelope Proteins / Q000235:genetics
D021382:Protein Sorting Signals; D012333:RNA, Messenger; D014759:Viral Envelope Proteins; D014364:Tryptophan
Konstruirovanie i svoĭstva iskusstvennykh politsistronov na osnove ukorochennykh genov triptofanovogo operona E. coli i belka obolochki faga M13.