Modulation of the murine immune response to streptococcal group A carbohydrate by immunization with monoclonal anti-idiotope.
Using monoclonal anti-idiotopes with previously defined specificities for the variable (V) domain of HGAC 39, a monoclonal antibody against streptococcal group A carbohydrate (GAC), we have studied the effect of anti-idiotope on an anticarbohydrate immune response. Anti-IdI-3a and anti-IdI-3b are anti-idiotopes which recognize binding site-associated determinants, whereas anti-IdX recognizes a framework-associated determinant on the HGAC 39 V kappa domain. Each of three anti-idiotopes elicited a specific idiotope response, as measured by inhibition radioimmunoassay, in A/J and C57BL/6J mice. A single immunization with conjugated anti-IdI-3a elicited an idiotope(+), GAC-binding(+) response in C57BL/6J and (BALB/c X CBA/N)F1 male mice, but not in A/J or (CBA/N X BALB/c)F1 male, X-linked immunodeficient mice. When C57BL/6J mice immunized initially with anti-idiotope were further treated with group A vaccine, those receiving anti-IdX had the greatest increase in anti-GAC activity. Stimulation of an anticarbohydrate response with anti-idiotope may therefore be enhanced by selecting anti-idiotopes against both binding site- and framework-associated determinants.
Monafo
W J
WJ
Department of Microbiology and Immunology, Washington University School of Medicine, St. Louis, MO 63110.
Greenspan
N S
NS
Cebra-Thomas
J A
JA
Davie
J M
JM
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000917:Antibody Formation; D001666:Binding Sites, Antibody; D007114:Immunization; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D007132:Immunoglobulin Isotypes / Q000276:immunology; D007153:Immunologic Deficiency Syndromes / Q000276:immunology; D051379:Mice; D008817:Mice, Mutant Strains / Q000276:immunology; D011135:Polysaccharides, Bacterial / Q000276:immunology*; D013297:Streptococcus pyogenes / Q000276:immunology*
D000911:Antibodies, Monoclonal; D007130:Immunoglobulin Idiotypes; D007132:Immunoglobulin Isotypes; D011135:Polysaccharides, Bacterial