title: The majority of "natural" immunoglobulin-secreting cells are short-lived and the progeny of cycling lymphocytes.
abstract: Treatment of mice with hydroxyurea to selectively kill all cycling cells has been used to study population dynamics and life expectancy of "natural" immunoglobulin-secreting cells in the bone marrow and spleen of nonimmunized animals. The results show that 50 to 90% of those cells are eliminated 2 to 3 days after one cycle of hydroxyurea administration, demonstrating their recent origin from cycling precursors. Using a protocol of long-term hydroxyurea treatment which abrogates cell production from the cycling precursors compartments, it was shown that "natural" immunoglobulin-secreting cells have a very short half renewal time, in the range of 15-60 h.
authors.forename: M
authors.initials: M
authors.lastname: Lévy
authors.forename: P
authors.initials: P
authors.lastname: Vieira
authors.forename: A
authors.initials: A
authors.lastname: Coutinho
authors.forename: A
authors.initials: A
authors.lastname: Freitas
mesh_terms: D000375:Aging / Q000097:blood; D000818:Animals; D000921:Antibody-Producing Cells / Q000166:cytology / Q000502:physiology*; D001402:B-Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D001854:Bone Marrow Cells; D002453:Cell Cycle / Q000187:drug effects; D002470:Cell Survival; D006462:Hemolytic Plaque Technique; D006918:Hydroxyurea / Q000008:administration & dosage / Q000276:immunology; D007074:Immunoglobulin G / Q000032:analysis; D007267:Injections; D008198:Lymph Nodes / Q000166:cytology; D008214:Lymphocytes / Q000166:cytology* / Q000187:drug effects / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008815:Mice, Inbred Strains; D013154:Spleen / Q000166:cytology; D013234:Stem Cells / Q000187:drug effects
chemical_list: D007074:Immunoglobulin G; D006918:Hydroxyurea