[22K and 20K growth hormone: correlation of receptor binding and the biological effect on rat adipocytes].
The 20K variant of native (22K) hGH is a full agonist for the growth promoting and lactogenic properties of the hormone in vivo, but has been reported to have reduced or no insulin-like properties. To explore whether these differences could be explained at the receptor level, we compared the binding of 22K and 20K hGH to receptors in isolated rat adipocytes, a target for the insulin-like effects of the hormone. We compared then the biological insulin-like effects of both hormones using a sensitive assay based on the stimulation of lipogenesis in isolated rat adipocytes. The 20K variant was only 3% as potent as 22K hGH for binding and bioactivity, demonstrating that rat adipocyte receptors are different from those promoting the growth effect.
Smal
J
J
Closset
J
J
Hennen
G
G
De Meyts
P
P
D000273:Adipose Tissue / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D013006:Growth Hormone / Q000502:physiology*; D019382:Human Growth Hormone; D007328:Insulin / Q000502:physiology; D008055:Lipids / Q000096:biosynthesis; D010446:Peptide Fragments / Q000502:physiology; D051381:Rats; D011986:Receptors, Somatotropin / Q000378:metabolism*
D007328:Insulin; D008055:Lipids; D010446:Peptide Fragments; D011986:Receptors, Somatotropin; C039519:somatotropin 20K; D019382:Human Growth Hormone; D013006:Growth Hormone
Hormone de croissance 22K et 20K: corrélation entre liaison au récepteur et effet biologique direct sur adipocyte de rat.