title: Immunoperoxidase staining of fine-needle aspiration biopsies of renal cell carcinoma using tumor-specific monoclonal antibody.
abstract: In 30 nephrectomy specimens, fine needle aspiration biopsies (FNAB) were performed both in the tumor and in the macroscopically normal part of the kidney: 18 were well differentiated renal cell carcinoma (RCC), 6 were moderately differentiated RCC, 4 were poorly differentiated RCC, and 2 were oncocytomas. FNAB was also performed in a bone metastasis of RCC. FNAB materials were stained using the immunoperoxidase method with RCC-specific monoclonal antibody and were compared with the staining of frozen sections. In all cases where tumor-antigen expression could be demonstrated in the frozen sections, a FNAB had already proven positive. There were only 2 false-negative cases where sufficient cellular material could not be obtained due to extensive tumor necrosis. There was no false-positive case. Oncocytomas showed no expression of antigen. The use of immunoperoxidase staining of FNAB with tumor-specific monoclonal antibody is discussed.
authors.affiliation: Department of Urology, Mainz University, FRG.
authors.forename: T
authors.initials: T
authors.lastname: Schärfe
authors.forename: M
authors.initials: M
authors.lastname: Yokoyama
authors.forename: P
authors.initials: P
authors.lastname: Alken
authors.forename: G H
authors.initials: GH
authors.lastname: Jacobi
authors.forename: R
authors.initials: R
authors.lastname: Hohenfellner
mesh_terms: D000236:Adenoma / Q000473:pathology; D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm / Q000032:analysis*; D001707:Biopsy, Needle; D002292:Carcinoma, Renal Cell / Q000473:pathology* / Q000556:secondary; D006801:Humans; D007124:Immunoenzyme Techniques; D007680:Kidney Neoplasms / Q000473:pathology*
chemical_list: D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm