A physiological role for luteinizing hormone release-inhibiting factor of hypothalamic origin.
A 12 KD peptide, LH release-inhibiting factor (LHRIF), extracted from rat hypothalamus has been shown to inhibit LHRH-stimulated LH release from cultured anterior pituitary cells. In the present study this partially purified material also blocks LHRH-stimulated LH release in pentobarbital-blocked proestrous rats. Administration of a polyclonal antiserum to partially purified LHRIF potentiates estrogen-induced LH release and antagonizes the inhibitory effect of estrogen and progesterone on LH release in ovariectomized rats. These results suggest that a hypothalamic LHRIF plays a physiological role in regulating LH release in the rat.
Hwan
J C
JC
Freeman
M E
ME
D000818:Animals; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000037:antagonists & inhibitors*; D007028:Hypothalamic Hormones / Q000276:immunology / Q000494:pharmacology*; D007031:Hypothalamus / Q000032:analysis*; D007106:Immune Sera / Q000494:pharmacology; D007700:Kinetics; D007986:Luteinizing Hormone / Q000378:metabolism*; D010052:Ovariectomy; D010424:Pentobarbital / Q000494:pharmacology; D011359:Proestrus / Q000378:metabolism; D011374:Progesterone / Q000494:pharmacology; D051381:Rats
D007028:Hypothalamic Hormones; D007106:Immune Sera; D007987:Gonadotropin-Releasing Hormone; D011374:Progesterone; D004958:Estradiol; D007986:Luteinizing Hormone; D010424:Pentobarbital