Murine monoclonal antibody against aldosterone: production, characterization and use for enzymoimmunoassay.
Hybridomas secreting monoclonal antibodies to aldosterone were obtained by fusion of myeloma cells and spleen cells from Balb/c mice immunized with aldosterone-3-carboxylmethyloxime-bovine serum albumin. A monoclonal antibody was purified from ascites fluid and characterized. An affinity constant of 1.61 x 10(9) M-1 has been measured and no cross-reactivity with tetrahydroaldosterone (THA), cortisol, cortisone, corticosterone, deoxycorticosterone (DOC), dehydroepiandrosterone (DHA), progesterone and estrone, could be detected. A peroxidase conjugated-antibody (1.5 mole of enzyme per mole of antibody) was obtained and used for microwell enzyme immunoassay and Immun-Blot assay. The high affinity and specificity of this antibody should make the direct determination of aldosterone in biological fluids possible at concentrations as low as 5 x 10(-10) M.
de Lauzon
S
S
Unité Associée 586 du Centre National de la Recherche Scientifique, Faculté de Médecine, Paris, France.
Le Trang
N
N
Moreau
M F
MF
Gentin
M
M
Christeff
N
N
Desfosses
B
B
Cittanova
N
N
D000450:Aldosterone / Q000032:analysis* / Q000276:immunology; D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification; D005260:Female; D006825:Hybridomas / Q000276:immunology; D007124:Immunoenzyme Techniques; D007202:Indicators and Reagents; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011863:Radioimmunoassay / Q000379:methods
D000911:Antibodies, Monoclonal; D007202:Indicators and Reagents; D000450:Aldosterone