Comparison of the properties of the purified beta-glucosidase from the transformed strain of Saccharomyces cerevisiae TYKF2 with that of the donor strain Kluyveromyces fragilis Y610.
Saccharomyces cerevisiae TYKF2 was obtained by cloning in S. cerevisiae the gene coding for beta-glucosidase in Kluyveromyces fragilis Y610 (ATCC 12424). The beta-glucosidases of both organisms were purified and their biochemical characteristics were determined. The two beta-glucosidases had the same enzymatic properties as those previously described in the literature. The strain S. cerevisiae TYKF2 is able to produce enhanced amounts of enzyme.
Leclerc
M
M
Chaire de Génétique et Microbiologie, ENSA-INRA, Montpellier, France.
Chemardin
P
P
Arnaud
A
A
Ratomahenina
R
R
Galzy
P
P
Gerbaud
C
C
Raynal
A
A
Guérineau
M
M
D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D005800:Genes, Fungal; D005959:Glucosidases / Q000235:genetics* / Q000302:isolation & purification; D007716:Kluyveromyces / Q000201:enzymology* / Q000235:genetics; D008970:Molecular Weight; D011489:Protein Denaturation; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics; D004718:Saccharomycetales / Q000201:enzymology*; D013816:Thermodynamics; D014170:Transformation, Genetic
D005959:Glucosidases