Construction of a yeast strain devoid of mitochondrial introns and its use to screen nuclear genes involved in mitochondrial splicing.
We have constructed a respiring yeast strain devoid of mitochondrial introns to screen nuclear pet- mutants for those that play a direct role in mitochondrial intron excision. Intron-less mitochondria are introduced by cytoduction into pet- strains that have been made rho0; cytoductants therefrom recover respiratory competency if the original pet- mutation is required only for mitochondrial splicing. By this means, we have identified 11 complementation groups of such genes. Their total number may be estimated as about 18.
Séraphin
B
B
Institut Curie, Centre Universitaire, Orsay, France.
Boulet
A
A
Simon
M
M
Faye
G
G
D002467:Cell Nucleus / Q000502:physiology*; D004272:DNA, Mitochondrial / Q000235:genetics*; D005800:Genes, Fungal; D005838:Genotype; D007438:Introns; D008928:Mitochondria / Q000502:physiology*; D009154:Mutation; D012326:RNA Splicing; D012331:RNA, Fungal / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D004272:DNA, Mitochondrial; D012331:RNA, Fungal