Effects of hormonal growth factors on colony formation and chemosensitivity of human tumors in agarose.
Because low plating efficiencies of most human cancers severely limit the number of successful chemosensitivity tests that can be performed, we studied the growth-enhancing effects of hormonal growth factors on a variety of solid tumors. Dose-response studies with progesterone and estradiol indicated no benefit from adding these substances to the culture medium. This was true whether progesterone or estradiol was used alone or in the presence of other hormones. By contrast, epidermal growth factor (EGF) at concentrations from 10 to 100 ng/ml increased colony numbers up to 10-fold. Although insulin, hydrocortisone, and EGF used alone could either stimulate or inhibit the growth of specific tumors, the combination of all three (hormone mixture or HM) was always at least as good and usually better than any individual component in increasing cloning efficiency. HM-supplemented medium gave significantly increased colony counts in 41/46 tumors. Sensitivity to anticancer drugs was not changed in 63 paired drug tests.
Kern
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DH
Surgical Service, Veterans Administration Medical Center, Sepulveda, California 91343.
Chien
F W
FW
Goodwein
S N
SN
Sondak
V K
VK
D000970:Antineoplastic Agents / Q000494:pharmacology; D002460:Cell Line; D003114:Colony-Forming Units Assay / Q000379:methods; D003470:Culture Media; D004305:Dose-Response Relationship, Drug; D004338:Drug Combinations; D004815:Epidermal Growth Factor / Q000494:pharmacology; D004958:Estradiol / Q000494:pharmacology; D006133:Growth Substances / Q000494:pharmacology*; D006801:Humans; D006854:Hydrocortisone / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology; D011374:Progesterone / Q000494:pharmacology; D012685:Sepharose; D014407:Tumor Cells, Cultured / Q000187:drug effects*; D014410:Tumor Stem Cell Assay / Q000379:methods
D000970:Antineoplastic Agents; D003470:Culture Media; D004338:Drug Combinations; D006133:Growth Substances; D007328:Insulin; D011374:Progesterone; D004958:Estradiol; D004815:Epidermal Growth Factor; D012685:Sepharose; D006854:Hydrocortisone