Monoclonal antibodies that recognize transcription unit proteins on newt lampbrush chromosomes.
We prepared hybridoma cell lines from mice injected with newt germinal vesicle proteins. We tested culture supernates from these cell lines for antibodies that bound to specific morphological structures in lampbrush chromosome preparations (nucleoli, loops, chromomeres, etc.). Four mAbs that recognize antigens on the lateral transcription loops are described here. We suggest that these antigens are proteins associated with nascent RNA transcripts, although they are not among the 30-40-kD "core" heterogeneous nuclear ribonucleoproteins.
Roth
M B
MB
Department of Embryology, Carnegie Institution, Baltimore, Maryland 21210.
Gall
J G
JG
D000818:Animals; D000911:Antibodies, Monoclonal; D002875:Chromosomes / Q000648:ultrastructure*; D005260:Female; D005455:Fluorescent Antibody Technique; D006825:Hybridomas / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains; D010053:Ovary / Q000166:cytology; D012455:Salamandridae; D014157:Transcription Factors / Q000032:analysis*; D014158:Transcription, Genetic
D000911:Antibodies, Monoclonal; D014157:Transcription Factors