Monoclonal antibodies against pig transferrin. Blocking and binding activity.
Monoclonal mouse anti-pig transferrin antibodies PTF-01, PTF-02 and PTF-03 and anti-human transferrin antibody HTF-14 detect transferrin coupled with Sepharose particles in an indirect immunofluorescence test. Only the PTF-03 antibody can be used for immunofluorescence detection of pig transferrin bound to specific receptors on the plasma membrane. The binding of iodinated pig transferrin to PK cells was studied. It could be blocked by non-labelled transferrin in excess, by pig serum or by anti-pig transferrin monoclonal antibodies. PTF-03 expressed the lowest blocking activity among the antibodies tested.
Trebichavský
I
I
Institute of Microbiology, Czechoslovak Academy of Sciences, Prague.
Macků
B
B
Viklický
V
V
Koukal
M
M
Siman
P
P
D000818:Animals; D000831:Animals, Newborn; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D005455:Fluorescent Antibody Technique; D006825:Hybridomas / Q000276:immunology; D008214:Lymphocytes / Q000378:metabolism*; D011990:Receptors, Transferrin / Q000378:metabolism*; D013552:Swine; D013556:Swine, Miniature; D014168:Transferrin / Q000276:immunology / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D011990:Receptors, Transferrin; D014168:Transferrin