Association of an abnormality of neutrophil chemotaxis in human periodontal disease with a cell surface protein.
Localized juvenile periodontitis (LJP) is characterized by severe, early-onset, molar and incisor bone loss; neutrophil chemotaxis disorders; and a high prevalence of Actinobacillus actinomycetemcomitans infection. LJP is further characterized by significant familial aggregation of the disease. Recent work in our laboratory has demonstrated the selective depletion of a surface glycoprotein of 110,000 Mr (GP110) from LJP neutrophils by using surface labeling with [14C]formaldehyde and autofluorography. The function of GP110 is unknown; however, it does not appear to be a chemotactic factor receptor. Rather, it is bound by a monoclonal antibody (NCD-1) that recognizes a neutrophil differentiation antigen and which itself alters neutrophil chemotactic and secreting functions. To quantify GP110 on LJP and normal neutrophils, fluorescein-labeled NCD-1 was bound to neutrophils and the amount of fluorescence was evaluated by using cytofluorography. Our results indicate that there is a quantifiable reduction (40%) of GP110 on the surface of LJP and GJP neutrophils, compared with controls. Other patients with neutrophil defects express normal quantities of GP110, suggesting disease specificity. Our data suggest that GP110 may be a useful disease marker for LJP and may provide a useful probe for the study of neutrophil chemotactic function and dysfunction.
Van Dyke
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Wilson-Burrows
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Offenbacher
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Henson
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D010520:Aggressive Periodontitis / Q000503:physiopathology*; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000032:analysis; D002634:Chemotaxis, Leukocyte; D006023:Glycoproteins / Q000502:physiology*; D006801:Humans; D007163:Immunosorbent Techniques; D008970:Molecular Weight; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000502:physiology*; D010510:Periodontal Diseases / Q000503:physiopathology*; D011951:Receptors, Complement / Q000032:analysis
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D006023:Glycoproteins; D011951:Receptors, Complement; D009240:N-Formylmethionine Leucyl-Phenylalanine