[Genetic study of plasmid integration into yeast chromosomes. V. Mapping of integration sites for the plasmid pYF91].
The data on mapping the episomal plasmid integration sites in yeast chromosomes I, III, IV, V, VII, XV are presented. In addition to the integration site at leu2 of chromosome III localized earlier, 6 more loci containing apparently the homologous yeast transposons, with a copy in a plasmid, were defined. The fact of plasmid integration was proved by colony hybridization technique with the pBR322 probe. The plasmid DNA segregation (the ratio 2:2) and its linkage to pLEU2 plasmid marker gene were observed in hybrids of all integrants studied.
Bulat
S A
SA
Mezhevaia
E V
EV
Stepanova
V P
VP
Iarovoĭ
B F
BF
Zakharov
I A
IA
D002874:Chromosome Mapping; D004271:DNA, Fungal / Q000235:genetics; D005819:Genetic Markers; D010957:Plasmids; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D004271:DNA, Fungal; D005819:Genetic Markers
Geneticheskoe izuchenie integratsii plazmid v drozhzhevye khromosomy. Soobshchenie V. Kartirovanie saĭtov integratsii plazmidy pYF91.