Characterization of the secreted antigens of Mycobacterium bovis BCG: comparison of the 46-kilodalton dimeric protein with proteins MPB64 and MPB70.
Western blot analysis showed that the 46-kilodalton (kDa) dimeric protein antigen secreted in large amounts by some daughter strains of Mycobacterium bovis BCG corresponded to protein MPB70 present in long-term culture filtrates of the Japanese substrain. The 46/23-kDa antigen is the most abundant protein in supernatant from a 5-day culture but is masked by leaked products in old culture supernatants. No similarities were found between the 46-kDa protein and MPB64, a protein with the same strain distribution, or with the antigen of similar molecular mass recognized by monoclonal antibody SA1.D2D.
Abou-Zeid
C
C
Department of Microbiology, School of Pathology, Middlesex Hospital Medical School, London, United Kingdom.
Harboe
M
M
Rook
G A
GA
D000911:Antibodies, Monoclonal / Q000276:immunology; D000942:Antigens, Bacterial / Q000032:analysis*; D001426:Bacterial Proteins / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D007163:Immunosorbent Techniques; D046911:Macromolecular Substances; D008970:Molecular Weight; D009163:Mycobacterium bovis / Q000276:immunology* / Q000378:metabolism
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D046911:Macromolecular Substances