title: Frog brain and liver show evolutionary conservation of tissue-specific differences among insulin receptors.
abstract: The insulin receptors of frog brain and liver show features typical of other insulin receptors with regard to affinity and specificity of binding to insulins and proinsulin, solubility in Triton X-100, binding to and elution from wheat germ agglutinin, and insulin-sensitive tyrosine kinase activity. Likewise, the brain and liver receptors differ from one another in electrophoretic mobility and susceptibility to treatment with neuraminidase, analogous to brain and liver receptors of reptiles, birds, and mammals; while the functional implications of these differences are unknown, their evolutionary conservation for 400-500 million years suggests the possibility that they might have importance.
authors.affiliation: Diabetes Branch, National Institute of Diabetes, Digestive and Kidney Diseases, Bethesda, Maryland 20892.
authors.forename: C
authors.initials: C
authors.lastname: Hart
authors.forename: J
authors.initials: J
authors.lastname: Shemer
authors.forename: J C
authors.initials: JC
authors.lastname: Penhos
authors.forename: M A
authors.initials: MA
authors.lastname: Lesniak
authors.forename: J
authors.initials: J
authors.lastname: Roth
authors.forename: D
authors.initials: D
authors.lastname: LeRoith
mesh_terms: D000818:Animals; D005075:Biological Evolution; D001921:Brain / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D066298:In Vitro Techniques; D007328:Insulin / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D017830:Octoxynol; D010766:Phosphorylation; D011092:Polyethylene Glycols; D011894:Rana pipiens; D011972:Receptor, Insulin / Q000378:metabolism*; D014909:Wheat Germ Agglutinins / Q000378:metabolism
chemical_list: D007328:Insulin; D014909:Wheat Germ Agglutinins; D011092:Polyethylene Glycols; D017830:Octoxynol; D011972:Receptor, Insulin