title: Purification of the lipopolysaccharide fraction from Klebsiella pneumoniae O1 K2 by high-performance liquid chromatography.
abstract: An high-performance liquid chromatography technique was applied to purify the lipopolysaccharide fraction from a lysate of Klebsiella pneumoniae O1 K2 (NCTC 5055). The separation of the lipopolysaccharide fraction from the proteins was carried out with a reversed-phase column. By this method the lipopolysaccharide fraction was obtained in a pure state, devoid of proteins but possessing the same biological properties as the lipopolysaccharide fraction prepared by the classical phenol-water technique.
authors.forename: O
authors.initials: O
authors.lastname: Kol
authors.forename: J
authors.initials: J
authors.lastname: Montreuil
authors.forename: B
authors.initials: B
authors.lastname: Fournet
authors.forename: R
authors.initials: R
authors.lastname: Zalisz
authors.forename: P
authors.initials: P
authors.lastname: Smets
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis; D002241:Carbohydrates / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D005227:Fatty Acids / Q000032:analysis; D007711:Klebsiella pneumoniae / Q000032:analysis*; D008070:Lipopolysaccharides / Q000302:isolation & purification*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D001426:Bacterial Proteins; D002241:Carbohydrates; D005227:Fatty Acids; D008070:Lipopolysaccharides