title: Perforin is present only in normal activated Lyt2+ T lymphocytes and not in L3T4+ cells, but the serine protease granzyme A is made by both subsets.
abstract: We show that among the subsets of peripheral T lymphocytes (Lyt2+ L3T4- and L3T4+ Lyt2- cells) activated in short-term cultures by stimulation with H-2 incompatible leukocytes 97% of the cytolytic activity and all detectable perforin activity resides in the Lyt2+ cells. But both populations contain approximately equal amounts of a serine protease, granzyme A, the expression of which was previously thought to be restricted to cytolytic T lymphocytes.
authors.forename: J A
authors.initials: JA
authors.lastname: Garcia-Sanz
authors.forename: G
authors.initials: G
authors.lastname: Plaetinck
authors.forename: F
authors.initials: F
authors.lastname: Velotti
authors.forename: D
authors.initials: D
authors.lastname: Masson
authors.forename: J
authors.initials: J
authors.lastname: Tschopp
authors.forename: H R
authors.initials: HR
authors.lastname: MacDonald
authors.forename: M
authors.initials: M
authors.lastname: Nabholz
mesh_terms: D000818:Animals; D000950:Antigens, Ly / Q000032:analysis*; D002478:Cells, Cultured; D003594:Cytoplasmic Granules / Q000201:enzymology / Q000648:ultrastructure; D003602:Cytotoxicity, Immunologic; D010450:Endopeptidases / Q000378:metabolism*; D053804:Granzymes; D006461:Hemolysis; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D012697:Serine Endopeptidases; D013601:T-Lymphocytes / Q000145:classification / Q000201:enzymology / Q000276:immunology*
chemical_list: D000950:Antigens, Ly; D010450:Endopeptidases; D053804:Granzymes; D012697:Serine Endopeptidases