Isomyosin expression in developing chicken atria: a marker for the development of conductive tissue?
Isomyosin expression patterns in embryonic chicken atria during the first two weeks of development were analyzed immunohistochemically. In the 3-days embryonic chicken heart (HH19-20), strong coexpression of both isomyosins can be found as band-like zones at the lateral sides of the sinoatrial junction. The zones converge on the bottom of the atrium and continue as a band around the atrioventricular canal. In the 5-days heart (HH27-28) the coexpression area encompasses the entire sinoatrial junction and extends into parts of the sinus venosus and into the dorsocaudal atrial wall. In the 7-days heart (HH 32-33) the relative extension of coexpression areas reaches its maximum. Coexpression is also found in a ring-like band in the ventral (bottom) wall of the atria peripheral to the ring-like band in the atrioventricular junction. The latter band has now become continuous with the coexpression area in the bottom of the interatrial septum. Caudally coexpression extends behind the atrioventricular cushions towards the interventricular septum and cranially coexpression of the atrioventricular junction has become continuous with that of the ring around the outflow tract (cf Sanders et al. 1986). In the second week of incubation a decrease of coexpression is observed. The isomyosin expression pattern described in this study has put forward additional arguments that the conductive tissue originates from areas that continue to express both isomyosins relatively late in development.
de Groot
I J
IJ
Department of Anatomy and Embryology, University of Amsterdam, The Netherlands.
Sanders
E
E
Visser
S D
SD
Lamers
W H
WH
de Jong
F
F
Los
J A
JA
Moorman
A F
AF
D000818:Animals; D002642:Chick Embryo; D006329:Heart Conduction System / Q000196:embryology / Q000378:metabolism*; D007124:Immunoenzyme Techniques; D009218:Myosins / Q000378:metabolism*
D009218:Myosins