title: [Cytochemical method for detecting dehydrogenase activity in the leukocytes of native blood smears].
abstract: A cytochemical method of detection of dehydrogenases in blood leucocytes is proposed. Native smears are dried up in the air to be incubated at 37 degrees C in gel-containing medium composed of polyvinyl alcohol, sucrose, a corresponding substrate, cofactors and inhibitors of cytochrome oxidases activity. Using corresponding media, activities of succinate, malate, glutamate, lactate-, alpha-glycerophosphate, alcohol, beta-oxybutyrate and glucoso-5-phosphate dehydrogenases were revealed. Half-reduced diformazan providing diffuse rosy staining of cells was removed after the incubation, and the incubation medium was washed out by rinsing the smears in 60% acetone solution. As a result monochromatic micropreparations may be received. Finally, smears are fixed in formalin. The above method provides a reduced loss of enzymes, preserves a good cell morphology and eliminates non-dehydrogenase effects of tetrazolium reduction into formazan.
authors.forename: E G
authors.initials: EG
authors.lastname: Bykov
authors.forename: V G
authors.initials: VG
authors.lastname: Chaĭtsev
authors.forename: A V
authors.initials: AV
authors.lastname: Petrov
authors.forename: N L
authors.initials: NL
authors.lastname: Gurevich
mesh_terms: D000818:Animals; D001801:Blood Stains; D003584:Cytological Techniques; D006651:Histocytochemistry; D006801:Humans; D007962:Leukocytes / Q000201:enzymology*; D010088:Oxidoreductases / Q000097:blood*; D013778:Tetrazolium Salts
chemical_list: D013778:Tetrazolium Salts; D010088:Oxidoreductases
vernacular_title: Tsitokhimicheskaia metodika vyiavleniia aktivnosti degidrogenaz v leĭkotsitakh nativnykh mazkov krovi.