title: Crystals of seryl-tRNA synthetase from Escherichia coli. Preliminary crystallographic data.
abstract: Crystals of seryl-tRNA synthetase from Escherichia coli can be grown from ammonium sulphate/octyl glucoside solutions in two days. The crystals appear to be very suitable for X-ray analysis, diffracting to at least 2.8 A resolution and being resistant to radiation damage. The crystals are monoclinic (space group C2) with cell parameters a = 148.2 A, b = 90.6 A, c = 69.5 A and beta = 119.0 degrees. Depending on whether the asymmetric unit is the enzyme monomer (Mr 48,414) or dimer the Vm value would be either 4.12 or 2.10 A3/dalton. Although the former would indicate a rather high solvent content, other proteins crystallized in the presence of octyl glucoside have Vm values similar to this.
authors.forename: R
authors.initials: R
authors.lastname: Leberman
authors.forename: C
authors.initials: C
authors.lastname: Berthet-Colominas
authors.forename: S
authors.initials: S
authors.lastname: Cusack
authors.forename: M
authors.initials: M
authors.lastname: Härtlein
mesh_terms: D000604:Amino Acyl-tRNA Synthetases; D003461:Crystallography; D004926:Escherichia coli / Q000201:enzymology*; D012714:Serine-tRNA Ligase
chemical_list: D000604:Amino Acyl-tRNA Synthetases; D012714:Serine-tRNA Ligase