title: Immunochemical demonstration of the clonal organization of chimaeric mouse epidermis.
abstract: The clonal organization of chimaeric mouse epidermis was demonstrated by in situ staining of whole-mount preparations using monoclonal antibodies directed against H-2k and H-2b antigens. A striking pattern of transversely oriented stripes or patches was found which extended from mid-dorsum to the flank region. The orientation of these patches indicates a preferred directional expansion of clones during the development of dorsal/lateral epidermis. The clonal pattern of the belly region differed in that stripes were not found, but a marked ventral midline boundary was observed. This demarcation line may be due to a physical effect, i.e. isolation of the left and right ventral halves of the epidermis during early embryogenesis with relatively little cell mingling following closure of the abdominal wall. The obvious nonhomogenous distribution of chimaeric components in dorsal/lateral and ventral epidermis contradicts assumptions of homogenous, fine-grained patchiness derived from electrophoretic analysis of tissue samples and used in studies of skin carcinogenesis. The observation that hair follicles may contain cells of both parental genotypes implies a polyclonal origin. Epidermal proliferative units as described by Potten (1974) were not revealed by the pattern of mosaicism at the cellular level in these chimaeric tissue sheets. This indicates that the proliferative compartment of each putative epidermal unit is polyclonal.
authors.affiliation: Institute of Cancer Research, Haddow Laboratories, Sutton, Surrey, UK.
authors.forename: G H
authors.initials: GH
authors.lastname: Schmidt
authors.forename: M A
authors.initials: MA
authors.lastname: Blount
authors.forename: B A
authors.initials: BA
authors.lastname: Ponder
mesh_terms: D000818:Animals; D002678:Chimera; D002999:Clone Cells; D000078404:Epidermal Cells; D007124:Immunoenzyme Techniques; D051379:Mice; D008815:Mice, Inbred Strains