Effect of immunization with Escherichia coli J5 on graft-versus-host disease induced by minor histocompatibility antigens in mice.
We have used a cell-wall-deficient mutant of Escherichia coli (E coli J5) to study the effect of active and passive immunization against bacterial endotoxin (ET) in a murine model of acute graft-versus-host disease (GVHD) induced by minor histocompatibility antigens. C57BL/B6 (H-2b) mice were irradiated and grafted with 1 X 10(7) anti-Thy-1.2 and complement-treated bone marrow cells and 5 X 10(7) spleen cells from LP/J (H-2b) donors. Groups of mice were immunized against J5--either actively immunized with killed J5 cells or pure J5 lipopolysaccharide or passively immunized with rabbit anti-J5 antiserum (R alpha J5). Controls included irradiation controls, negative controls (conventional mice grafted with depleted bone marrow only), positive controls (conventional mice grafted with bone marrow and spleen cells), and mice passively immunized with normal rabbit serum (NRS). Positive control mice developed GVHD and all died by day +77. Active immunization partially protected against the rapid weight loss due to GVHD (P less than 0.05) but mice had an earlier onset of GVHD (P less than 0.05) and a shortened survival after onset (P less than 0.05). Median survival time (MST) and overall survival were unchanged. Passive immunization with R alpha J5 delayed death (P less than 0.01), increased MST (P = 0.01), increased overall survival (P less than 0.01), and protected against weight loss (P = 0.01). NRS had no beneficial effect.
Moore
R H
RH
Lampert
I A
IA
Chia
Y
Y
Aber
V R
VR
Cohen
J
J
D000818:Animals; D001427:Bacterial Toxins / Q000276:immunology; D016026:Bone Marrow Transplantation; D004731:Endotoxins / Q000276:immunology*; D004926:Escherichia coli / Q000276:immunology*; D005260:Female; D006086:Graft vs Host Disease / Q000276:immunology*; D007114:Immunization; D007116:Immunization, Passive; D008070:Lipopolysaccharides / Q000276:immunology; D051379:Mice; D008912:Minor Histocompatibility Loci
D001427:Bacterial Toxins; D004731:Endotoxins; D008070:Lipopolysaccharides