Repair of UV-damaged DNA in mammalian skin followed by the immunohistochemical method.
DNA repair in murine and guinea pig skin has been studied by the immunohistochemical method. For the detection of DNA photolesions in situ by the indirect immunofluorescence (IF) method two antisera directed against DNA-pyrimidine-dimers and DNA-psoralen-photoadducts have been applied. The IF assay enabled to detect the DNA photodamage induced by high UV-doses, exceeding more than fivefold minimal phototoxic response of the skin. It was found that IF staining gradually disappeared due to DNA repair, and at 48 h after UV-exposure both types of the DNA photolesions were no more detectable. Importantly, the IF method revealed that the persistence of DNA-pyrimidine-dimers could be traced for a longer time than that detectable by UV-endonuclease incision method.
Zarebska
Z
Z
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warszawa.
Pathak
M A
MA
Jarzabek-Chorzelska
M
M
Wolska
H
H
Chorzelski
T
T
Jabłońska
S
S
D000818:Animals; D004249:DNA Damage; D004260:DNA Repair; D005455:Fluorescent Antibody Technique; D006168:Guinea Pigs; D051379:Mice; D008812:Mice, Hairless; D011701:PUVA Therapy / Q000009:adverse effects; D010787:Photosensitivity Disorders / Q000378:metabolism; D012867:Skin / Q000378:metabolism / Q000528:radiation effects*; D014466:Ultraviolet Rays / Q000009:adverse effects*