Active and inactive renin in the cat.
Pentobarbitone-anesthetized cats underwent peritoneal dialysis and had blood samples removed and kidneys deep frozen at sacrifice. Inactive renin is easily measurable in cat plasma and peritoneal dialysate fluid. Only small amounts are found after acid activation at pH 4.0, but large amounts after trypsin 2 mg/ml at 4 degrees C for 10 minutes. Mean active renin in pentobarbitone-anesthetized cats was 1.8 +/- 0.4 pmoles AI/ml/hr, while inactive renin was 2.3 +/- 0.5 pmoles AI/ml/hr. The increased angiotensin I producing activity after trypsin in peritoneal dialysate was most active at pH 7.0 (plasma and kidney active renin 7.25 and 7.85), and had an apparent molecular weight of 39-40,000. (Plasma active renin had an apparent MW of 33,500 and kidney active renin 36,000. Plasma inactive renin had an apparent MW of 35,500) Cat plasma after cibacron-blue affinity chromatography showed mainly active renin in the breakthrough buffer (30% of total renin eluted), and renin which is almost entirely inactive in the bound peak (70% of total renin eluted). Active renin from plasma and kidney, and activated inactive renin from concentrated peritoneal fluid, showed exactly similar inhibition by the renin inhibitor H77 (IC50 0.3 microM). Cat plasma angiotensinogen had an apparent MW of 53,000.
McKenzie
I M
IM
Department of Internal Medicine, University of Manitoba, Winnipeg, Canada.
Heiman
D
D
McKenzie
J K
JK
D000808:Angiotensinogen / Q000302:isolation & purification; D000818:Animals; D001202:Ascitic Fluid / Q000201:enzymology; D002415:Cats; D004789:Enzyme Activation / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D007668:Kidney / Q000201:enzymology; D008970:Molecular Weight; D012083:Renin / Q000097:blood / Q000378:metabolism*; D014357:Trypsin / Q000494:pharmacology; D000092502:Renin Inhibitors
D000808:Angiotensinogen; D012083:Renin; D014357:Trypsin; D000092502:Renin Inhibitors