Quality control and the preparative purification of low antibody concentrations for radiolabelling using bifunctional chelates.
The conjugation of antibodies (Ab) with DTPA in the presence of contaminating low molecular weight proteins is inefficient. The selective precipitation of Ab with 18% Na2SO4 as a preparative purification method for small aliquots of low Ab concentrations is successful in eliminating all contaminants as monitored by high performance liquid chromatography at 214 nm and in allowing IgG recoveries greater than 94%. Subsequently, this allows efficient conjugation and radiolabelling of Ab.
Zoghbi
S S
SS
Department of Diagnostic Radiology, Yale University School of Medicine, New Haven, CT 06510.
el Rassi
Z
Z
Neumann
R D
RD
Gottschalk
A
A
D000906:Antibodies / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D004369:Pentetic Acid; D011786:Quality Control; D011865:Radioisotope Dilution Technique / Q000592:standards*
D000906:Antibodies; D004369:Pentetic Acid