title: A yeast gene coding for a putative protein kinase homologous to cdc25 suppressing protein kinase.
abstract: A yeast gene termed YKR coding for a putative protein kinase was isolated by using the cloned cDNA for rabbit protein kinase C as a hybridization probe. The encoded protein (YKR), composed of 380 amino acid residues, shows extensive sequence homology to serine/threonine-specific protein kinases from various species in the approx. 320 C-terminal amino acid residues, strongly suggesting that YKR is endowed with a protein kinase activity. The observed homologies to the cdc25 suppressing protein kinase from yeast, the catalytic subunit of mammalian cAMP-dependent protein kinase, and mammalian protein kinase C were 76, 48 and 37%, respectively. Gene replacement experiments showed that YKR itself is not essential for cell proliferation.
authors.affiliation: Department of Molecular Biology, Tokyo Metropolitan Institute of Medical Science, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Ohno
authors.forename: M
authors.initials: M
authors.lastname: Aoshima
authors.forename: S
authors.initials: S
authors.lastname: Matsumoto
authors.forename: I
authors.initials: I
authors.lastname: Yahara
authors.forename: K
authors.initials: K
authors.lastname: Suzuki
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D004247:DNA / Q000032:analysis; D005796:Genes; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D011493:Protein Kinase C / Q000235:genetics; D011494:Protein Kinases / Q000235:genetics*; D011817:Rabbits; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D013489:Suppression, Genetic
chemical_list: D004247:DNA; D011494:Protein Kinases; D011493:Protein Kinase C