A simplified technique for low temperature methyl methacrylate embedding.
A simplified method for low temperature methyl methacrylate embedding with inhibited methyl methacrylate monomer is demonstrated using proper concentrations of benzoyl peroxide and N,N-dimethylaniline. The polymerized tissue blocks cut well and the tissue sections obtained show excellent acid phosphatase activity when demonstrated with the newly improved technique and Goldner's staining. Likewise, double tetracycline labels are well revealed by fluorescence microscopy.
Liu
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D000135:Acid Phosphatase / Q000032:analysis; D000814:Aniline Compounds; D000818:Animals; D001585:Benzoyl Peroxide; D001842:Bone and Bones / Q000033:anatomy & histology / Q000201:enzymology; D003080:Cold Temperature; D005260:Female; D006652:Histological Techniques; D008689:Methacrylates; D020366:Methylmethacrylate; D008768:Methylmethacrylates; D008867:Microtomy; D011108:Polymers; D051381:Rats; D011919:Rats, Inbred Strains
D000814:Aniline Compounds; D008689:Methacrylates; D008768:Methylmethacrylates; D011108:Polymers; D020366:Methylmethacrylate; C005044:hydroxyethyl methacrylate; C015157:N,N-dimethylaniline; D000135:Acid Phosphatase; D001585:Benzoyl Peroxide