The effects of three recombinant growth factors, IL-3, GM-CSF, and G-CSF, on the blast cells of acute myeloblastic leukemia maintained in short-term suspension culture.
The blast stem cells of acute myeloblastic leukemia (AML) respond in cell culture to growth factors by both self-renewal and terminal divisions. Both of these functions have been shown to be stimulated by the recombinant growth factors granulocyte-macrophage colony-stimulating factor (GM-CSF) and granulocyte colony-stimulating factor (G-CSF). In this paper, recombinant gibbon interleukin-3 (IL-3), homologous to human IL-3, was tested on blast cells and compared with the effects of GM-CSF, G-CSF, and medium conditioned by the bladder cell line 5637 (5637-CM). We found that IL-3 was an effective stimulator of blast renewal and terminal divisions. However, great patient-to-patient variation was found. A graphic method of presenting complex comparisons between growth factors is also included.
Miyauchi
J
J
Kelleher
C A
CA
Yang
Y C
YC
Wong
G G
GG
Clark
S C
SC
Minden
M D
MD
Minkin
S
S
McCulloch
E A
EA
D000208:Acute Disease; D001773:Blood Cells / Q000187:drug effects* / Q000473:pathology; D001853:Bone Marrow / Q000187:drug effects; D001854:Bone Marrow Cells; D002452:Cell Count; D002478:Cells, Cultured; D003584:Cytological Techniques; D006801:Humans; D007377:Interleukin-3 / Q000494:pharmacology*; D015470:Leukemia, Myeloid, Acute / Q000097:blood*; D008747:Methylcellulose; D011994:Recombinant Proteins / Q000494:pharmacology*; D011995:Recombination, Genetic; D014410:Tumor Stem Cell Assay
D007377:Interleukin-3; D011994:Recombinant Proteins; D008747:Methylcellulose