[Redistribution of bone marrow cells according to mitotic cycle phases after the action of agents altering the intracellular concentration of cyclic nucleotides].
The method of flow cytofluorometry was used to determine the distribution of murine bone marrow cells along the phases of the cell cycle in normal mice (CBA X C57Bl) F1 after the whole body equal X-ray irradiation, after irradiation in combination with burn, after administration of imidazole, insulin and caffeine, and after the treatment of cells by acetylcholine. In non-irradiated mice insulin and caffeine induced an increased ratio of bone marrow cells in G0 and G1 and a reduced one in S phase. Imidazole increased the number of G2- and M-cells by 1.5 times only in regenerating bone marrow of irradiated mice. After X-ray irradiation of mice at a dose of 2.3 Gy, reducing the percentage of cells in S and increasing it in G2 and M phases, insulin, acetylcholine and caffeine were found to decrease the number of cells in G2 and M phases: besides, caffeine and acetylcholine increased the percentage of S-phase cells. The data obtained are discussed in terms of possibility of normalization of bone marrow cell proliferation broken after exposure to physical stress-factors.
Gracheva
N I
NI
Pereverzev
A E
AE
Rozanov
Iu M
IuM
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D001853:Bone Marrow / Q000187:drug effects* / Q000528:radiation effects; D001854:Bone Marrow Cells; D002056:Burns / Q000473:pathology; D002110:Caffeine / Q000494:pharmacology; D002453:Cell Cycle / Q000187:drug effects / Q000528:radiation effects; D005434:Flow Cytometry; D007093:Imidazoles / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008938:Mitosis / Q000187:drug effects* / Q000528:radiation effects; D009712:Nucleotides, Cyclic / Q000378:metabolism* / Q000528:radiation effects
D007093:Imidazoles; D007328:Insulin; D009712:Nucleotides, Cyclic; D002110:Caffeine; C029899:imidazole; D000109:Acetylcholine
Pereraspredelenie kostnomozgovykh kletok po fazam mitoticheskogo tsikla posle deĭstviia agentov, izmeniaiushchikh vnutrikletochnuiu kontsentratsiiu tsiklicheskikh nukleotidov.