title: The ATP requiring step in assembly of M13 procoat protein into microsomes is related to preservation of transport competence of the precursor protein.
abstract: M13 procoat protein is processed to transmembrane coat protein by dog pancreas microsomes after completion of synthesis and in the absence of the signal recognition particle (SRP)/docking protein system. ATP is required for fast and efficient processing of procoat protein by microsomes in a reticulocyte lysate. Requirement for ATP is also observed in the absence of ribosomes or docking protein. This indicates the existence of a unique assembly pathway for procoat protein into microsomes which depends on ATP but does not depend on the SRP/docking protein and ribosome/ribosome receptor systems. We suggest that the ATP requirement is linked to a so far unknown component of the reticulocyte lysate, acting on transport competence of precursor proteins.
authors.forename: H
authors.initials: H
authors.lastname: Wiech
authors.forename: M
authors.initials: M
authors.lastname: Sagstetter
authors.forename: G
authors.initials: G
authors.lastname: Müller
authors.forename: R
authors.initials: R
authors.lastname: Zimmermann
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D002213:Capsid / Q000096:biosynthesis / Q000235:genetics*; D036022:Capsid Proteins; D003090:Coliphages / Q000235:genetics* / Q000378:metabolism; D004285:Dogs; D004721:Endoplasmic Reticulum / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D008861:Microsomes / Q000378:metabolism; D010179:Pancreas / Q000378:metabolism; D010957:Plasmids; D014176:Protein Biosynthesis; D011498:Protein Precursors / Q000096:biosynthesis / Q000235:genetics*; D011499:Protein Processing, Post-Translational; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D036022:Capsid Proteins; D011498:Protein Precursors; C025668:coat protein, Bacteriophage M13; D000255:Adenosine Triphosphate