Lymphocyte expression of a 90 kD brush border antigen.
This study analyses the expression by rat lymphocytes of six renal brush border (BB) antigens defined by a set of monoclonal antibodies and demonstrates that a 90 kD protein (gp 90), synthesized by BB and glomeruli is found on the surface of T and B cells. The other antigens, including the 330 kD protein involved in Heymann's nephritis--a model of epimembranous glomerulonephritis--are not detectable on the surface of lymphocytes. Immunochemical studies indicate that gp 90 and the related protein immunoprecipitated from thymocyte membranes co-migrate in SDS-PAGE. Quantitative binding analysis shows that the number of antigenic sites is in the same order of magnitude on thymocytes, spleen and bone-marrow lymphocytes as well as on two lines of pre-B and pre-T cells, but considerably lower on a highly differentiated helper T cell line.
Van Leer
E H
EH
Moullier
P
P
Ronco
P
P
Verroust
P
P
D000818:Animals; D001324:Autoantigens / Q000032:analysis*; D001402:B-Lymphocytes / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D005921:Glomerulonephritis / Q000276:immunology*; D007678:Kidney Glomerulus / Q000276:immunology*; D008871:Microvilli / Q000276:immunology; D051381:Rats; D013601:T-Lymphocytes / Q000276:immunology*
D001324:Autoantigens