Increased uptake and phosphorylation of 2-deoxyglucose by skeletal muscles in endotoxin-treated rats.
Glucose metabolism of respiratory and nonrespiratory muscles of different fiber composition was investigated in conscious rats. The accumulation of phosphorylated 2-deoxyglucose (2DGP) was increased in skeletal muscles by 56-102% and in diaphragm by 236% at 3 h after treatment with 100 micrograms/100 g endotoxin. The increase was still marked at 24 h, whereas it diminished at 48 h in the diaphragm, abdominal muscle, and white portion of the quadriceps. In the red portion of this muscle 2DGP accumulation was less than that in time-matched controls at 24 and 48 h. Whole gastrocnemius (mixed-fiber types) showed no changes after 24 h. The high 2DGP accumulation in brain remained stable. The retention of 2DGP in tissues, studied by sequential double labeling, did not change 3 h after endotoxin. The lumped constant was similar in the isolated epitrochlear muscles of endotoxemic and control rats. Whole-body glucose utilization (Rd) was increased by 68% 3 h after endotoxin, but it was normal at 24 and 48 h. The increase of glucose utilization by the entire skeletal muscle mass was responsible for approximately 25% of the increase in Rd; therefore it appears that other tissues also contributed significantly to the endotoxin-induced alterations in carbohydrate metabolism.
Mészáros
K
K
Bagby
G J
GJ
Lang
C H
CH
Spitzer
J J
JJ
D000818:Animals; D001921:Brain / Q000378:metabolism; D003837:Deoxy Sugars / Q000378:metabolism*; D003847:Deoxyglucose / Q000378:metabolism*; D004731:Endotoxins / Q000494:pharmacology*; D004926:Escherichia coli; D005947:Glucose / Q000378:metabolism; D006439:Hemodynamics / Q000187:drug effects; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D009132:Muscles / Q000378:metabolism*; D010766:Phosphorylation; D051381:Rats; D011919:Rats, Inbred Strains
D003837:Deoxy Sugars; D004731:Endotoxins; D003847:Deoxyglucose; D005947:Glucose