Whole-cell bacterial peroxidase test with isoproterenol as the hydrogen donor.
The beta-adrenergic compound isoproterenol was used as oxidizable reagent in a whole-cell assay for the detection of bacterial peroxidase activities. Isoproterenol has been shown to constitute a useful reagent for detecting peroxidase activities in enzymatic tests, utilizing standard purified enzymes, and in the microbiological application proposed. The procedure developed is simple and rapid to perform. In contrast to currently used whole-cell tests for bacterial peroxidases, the assay described here does not need preliminary permeabilization; moreover, the compound utilized does not have related toxicological problems. Therefore, the isoproterenol assay may represent a low-cost safe additional peroxidase test in clinical bacteriology.
Giunta
S
S
Sezione di Microbiologia, Instituto di Ricovero e Cura a Carattere Scientifico INRCA, Ancona Italy.
Turchetti
G
G
Galeazzi
L
L
Grilli
G
G
Groppa
G
G
Rocchetti
R
R
D001419:Bacteria / Q000201:enzymology*; D001431:Bacteriological Techniques; D004796:Clinical Enzyme Tests / Q000379:methods*; D006859:Hydrogen / Q000378:metabolism; D007545:Isoproterenol / Q000378:metabolism*; D010544:Peroxidases / Q000378:metabolism*
D006859:Hydrogen; D010544:Peroxidases; D007545:Isoproterenol