title: Aging effects of vitamin C on a human lens protein produced in vitro.
abstract: Human lens gamma-crystallin obtained from the expression of a gene construct stably integrated into mouse L cells was incubated with ascorbate in the presence of iron and oxygen. The resulting oxidation of the gamma-crystallin led to more acidic species of this protein. These alterations were similar to the changes seen with aging in the human lens. The results suggest that oxidation of lens crystallins may be responsible for the changes seen on aging and cataract development and that ascorbate may contribute to these alterations.
authors.forename: P
authors.initials: P
authors.lastname: Russell
authors.forename: D
authors.initials: D
authors.lastname: Garland
authors.forename: J S
authors.initials: JS
authors.lastname: Zigler
authors.forename: S O
authors.initials: SO
authors.lastname: Meakin
authors.forename: L C
authors.initials: LC
authors.lastname: Tsui
authors.forename: M L
authors.initials: ML
authors.lastname: Breitman
mesh_terms: D000375:Aging / Q000378:metabolism*; D001205:Ascorbic Acid / Q000494:pharmacology*; D003459:Crystallins / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007158:Immunologic Techniques; D066298:In Vitro Techniques; D007501:Iron / Q000494:pharmacology; D007525:Isoelectric Focusing; D008970:Molecular Weight; D010084:Oxidation-Reduction
chemical_list: D003459:Crystallins; D007501:Iron; D001205:Ascorbic Acid