Application of Biken test (modified Elek test) for sampling of heat-stable enterotoxin of Escherichia coli isolated in Bangladesh.
The usefulness of Biken Agar 2 as a source of heat-stable toxin for the suckling mouse assay was examined. Escherichia coli (E. coli) strains isolated in Bangladesh from patients with gastroenteritis found to produce heat-stable toxin (n = 152), both heat-stable and heat-labile toxin (n = 60) and not to produce heat-stable or heat-labile toxin (n = 25) by standard suckling mice assay using broth culture were tested. Sampling from Biken Agar 2 gave comparable results to those obtained using standard broth cultures. This is the first field survey of evaluation of the Biken test for sampling heat-stable toxin of E. coli. The result further clarifies the applicability of the Biken test for sampling heat-stable toxin, and the usefulness of the Biken test for detections of heat-labile and heat-stable toxin produced by E. coli.
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D000818:Animals; D000833:Animals, Suckling; D001427:Bacterial Toxins / Q000032:analysis* / Q000096:biosynthesis; D001459:Bangladesh; D003470:Culture Media; D004768:Enterotoxins / Q000032:analysis* / Q000096:biosynthesis; D004926:Escherichia coli / Q000378:metabolism*; D029968:Escherichia coli Proteins; D006801:Humans; D051379:Mice
D001427:Bacterial Toxins; D003470:Culture Media; D004768:Enterotoxins; D029968:Escherichia coli Proteins; C034151:heat stable toxin (E coli)