title: Immunocytochemical analysis of HLA class II (DR) antigens in liver disease in man.
abstract: The in situ distribution of the major histocompatibility (HLA) class II (DR) antigens was studied in 113 liver biopsy specimens and five livers obtained at necropsy, using monoclonal antibody CR3/43. In 20 normal livers HLA-DR antigens were not detected in bile duct epithelium, hepatocytes, or portal vein endothelium. Normal arteriolar, sinusoidal and central venous endothelium often expressed HLA-DR. Kupffer cells always expressed these antigens. HLA-DR positive spindle cells were identified in the connective tissue of portal tracts, large hepatic veins, and liver capsule: most shared antigens common to all leucocytes and reacted with the histiocytic maker EBM11. Bile duct epithelium expresses HLA-DR in primary biliary cirrhosis, large duct obstruction, and drug induced cholestasis, indicating that HLA-DR positive spindle cells are phenotypically similar to histiocytes.
authors.affiliation: Department of Histopathology, Lewisham Hospital, London.
authors.forename: C
authors.initials: C
authors.lastname: Barbatis
authors.forename: P
authors.initials: P
authors.lastname: Kelly
authors.forename: J
authors.initials: J
authors.lastname: Greveson
authors.forename: A
authors.initials: A
authors.lastname: Heryet
authors.forename: J O
authors.initials: JO
authors.lastname: McGee
mesh_terms: D000911:Antibodies, Monoclonal; D001652:Bile Ducts / Q000276:immunology; D001660:Biliary Tract Diseases / Q000276:immunology; D006681:HLA-D Antigens / Q000032:analysis*; D006684:HLA-DR Antigens / Q000032:analysis*; D006801:Humans; D007124:Immunoenzyme Techniques; D008099:Liver / Q000276:immunology*; D008107:Liver Diseases / Q000276:immunology*; D011169:Portal Vein / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D006681:HLA-D Antigens; D006684:HLA-DR Antigens