Biochemical and molecular effects of N-nitroso compounds in human cultured cells: an overview.
In-vitro models using human tissues and cells provide a bridge between studies of humans and of laboratory animals. Cultured human cells activate N-nitrosamines to DNA-damaging metabolites, including alkyldiazonium ions and aldehydes; these metabolites can also inactivate DNA repair processes, such as O6-alkylguanine DNA-alkyltransferase (AAT) activity, and are mutagenic in human cells. Both human epithelial and mesenchymal cells have been transformed in vitro by N-nitroso compounds.
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Laboratory of Human Carcinogenesis, National Cancer Institute, Bethesda, MD 20892.
D002471:Cell Transformation, Neoplastic; D002869:Chromosome Aberrations; D004247:DNA / Q000378:metabolism; D004249:DNA Damage / Q000187:drug effects*; D004260:DNA Repair / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D009154:Mutation; D009603:Nitroso Compounds / Q000378:metabolism / Q000633:toxicity*; D012906:Smoke / Q000032:analysis
D009603:Nitroso Compounds; D012906:Smoke; D004247:DNA