[Transplantation antigen specific rosette test and its inhibition with autologous serum--a new immunologic method for predicting rejection crisis following allogeneic organ transplantation].
A combination of transplantation-antigen-specific rosette test with inhibition of rosette formation, using autologous serum, was used in postoperative follow-up checks on patients who had undergone allogeneic kidney transplantation and proved successful in early diagnosis or prediction of rejection episodes even before clinical manifestation. The numbers of antigen-detecting and antigen-binding cells differed considerably from each other, depending on patients who had undergone kidney transplantation. Almost complete inhibition of antigen detection was generally achieved by addition of patient serum. If sensitization to alloantigen in excess of the limit value (30 RBZ/10(3) KHZ) is accompanied by rosette formation with autologous serum addition in excess of about 18 RBZ/10(3) KHZ, clinically relevant rejection can be expected to occur after another two or three days and will have to be treated by immunosuppressives. Such partial or even complete elimination of rosette inhibition is usually only of short length (one day) and has rarely been observed to last longer. No rejection calling for treatment will develop, on the other hand, as long as blocking serum factors provide for high-stability inhibition of any bond of transplantation antigen (rosette formation). Constantly low rosette formation with serum addition and without was exhibited by control persons (blood donors, patients with cerebrocranial trauma) in daily examinations. Limit values of 18 or 30 RBZ/10(3) KHZ were not surpassed.
Werner
M
M
Klinik für Chirurgie, Bereichs Medizin der Karl-Marx-Universität Leipzig.
Otto
U
U
Keitel
R
R
Klötzer
B
B
Walther
J
J
Kiene
S
S
Schulze
R
R
Langkopf
B
B
Ambrosius
H
H
Sauer
H
H
D006084:Graft Rejection; D006680:HLA Antigens / Q000276:immunology*; D006801:Humans; D007519:Isoantigens / Q000276:immunology; D016030:Kidney Transplantation; D011379:Prognosis; D012397:Rosette Formation; D014181:Transplantation Immunology
D006680:HLA Antigens; D007519:Isoantigens
Der Transplantationsantigen-spezifische Rosettentest und dessen Hemmung mit autologem Serum--Eine neue immunologische Methode zur Voraussage von Rejektionskrisen nach allogener Organtransplantation.