[A method of determining the level of provitamin D in Saccharomyces cerevisiae with altered sterol metabolism].
A method is proposed that allows to choose among the polyene-resistant mutants of the yeasts Saccharomyces cerevisiae with changed biosynthesis of ergosterol promising producers of provitamin D4 and structural analogs of provitamin D3. The method involves both UV-spectrophotometry and thin-layer chromatography. The results obtained are supported by the data of mass-spectrometry of sterols.
Chichikalova
M M
MM
Zhakovskaia
Z A
ZA
Moseĭchuk
A V
AV
Mikhalova
N P
NP
V'iunov
K A
KA
D002855:Chromatography, Thin Layer; D004352:Drug Resistance, Microbial; D009154:Mutation; D011355:Prodrugs; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet; D013261:Sterols / Q000378:metabolism*; D014807:Vitamin D / Q000096:biosynthesis*
D011355:Prodrugs; D013261:Sterols; D014807:Vitamin D
Metodika otsenki soderzhaniia provitaminov gruppy D v drozhzhakh Saccharomyces cerevisiae s izmenennym metabolizmom sterinov.