The effect of insulin on progesterone production and cellular growth in long-term cultures of human granulosa lutein cells.
The direct action of insulin on human granulosa lutein cells (GLCs) in long-term cultures obtained from in vitro fertilization (IVF) cycles was investigated. Progesterone (P) secretion by GLC increased progressively in both basal and human chorionic gonadotropin (hCG; 100 mIU/ml) stimulated conditions up to 4 days in culture, and plateaued thereafter. Insulin (0.0025 mU/ml to 2500 mU/ml) had no effect on either basal or hCG stimulation during the culture period. GLC in culture formed a monolayer and multiplied at a rate of approximately once every 3 days. Neither morphology nor cell division was affected by insulin in supraphysiologic levels (25 mU/ml). These results suggest that GLC obtained from preovulatory follicles in an IVF program are already stimulated maximally by in vivo exposure to high doses of human menopausal gonadotropin (hMG)/hCG administered to the women. Contrary to its stimulatory effect on early preovulatory granulosa cells, insulin dose not affect P production, cellular morphology, or growth rate of luteinized granulosa cells.
Hurwitz
A
A
Department of Obstetrics and Gynecology, Hadassah University Hospital, Jerusalem, Israel.
Laufer
N
N
Keshet
I
I
Rabinowitz
R
R
Lewin
A
A
Palti
Z
Z
Schenker
J G
JG
D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D003338:Corpus Luteum / Q000378:metabolism*; D005260:Female; D006801:Humans; D007328:Insulin / Q000494:pharmacology*; D007700:Kinetics; D008184:Luteal Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D008938:Mitosis / Q000187:drug effects; D011374:Progesterone / Q000096:biosynthesis*
D006063:Chorionic Gonadotropin; D007328:Insulin; D011374:Progesterone