An avidin-biotin micro-enzymeimmunoassay for monkey chorionic gonadotrophin.
The avidin-biotin micro-enzymeimmunoassay used antiserum to the beta-subunit of sheep LH, biotinylated hCG as tracer and pooled serum from pregnant monkeys as a reference preparation of monkey CG. The assay was validated for specificity, by checking serum samples known to contain high quantities of LH, and false positive results were eliminated to a great extent by prior heating of serum samples at 60 degrees C for 30 min. In 45 bonnet monkeys the test gave 10 true positives, 34 true negatives and 1 false positive, i.e. an accuracy of 97%. The serum profiles of CG, measured by the EIA, in bonnet, cynomolgus and rhesus monkeys during early pregnancy were very similar to those reported for bonnet and rhesus monkeys when a liquid-phase radioimmunoassay was used.
Chakrabarti
R
R
Rao
A J
AJ
D000818:Animals; D001360:Avidin; D001710:Biotin; D006063:Chorionic Gonadotropin / Q000032:analysis*; D005260:Female; D007124:Immunoenzyme Techniques; D008251:Macaca / Q000097:blood* / Q000502:physiology*; D008252:Macaca fascicularis; D008253:Macaca mulatta / Q000502:physiology*; D008255:Macaca radiata / Q000097:blood*; D011247:Pregnancy
D006063:Chorionic Gonadotropin; D001360:Avidin; D001710:Biotin