title: A survey of anti-platelet antibody detection--report of the UK Platelet and Granulocyte Serology Working Group, 1985.
abstract: In 1985, serum samples were distributed to 27 laboratories within the UK and Eire with the objective of determining the current state of anti-platelet antibody detection. The sera included five unknown samples for blind assessment and four well-characterized reference reagents. The laboratories involved used a wide range of different methods but the most commonly used techniques were the fluorescent antiglobulin test and the enzyme-linked immunosorbent assay. In the trial, all responding laboratories were able to detect potent anti-platelet alloantibodies but there was a 43% incidence of 'false positive' results with samples which had been designated as inert. There were no apparent differences in performance between assays using fluorescein- or enzyme-labelled antiglobulin reagents or between assays which utilized PFA-treated rather than untreated platelets.
authors.forename: G F
authors.initials: GF
authors.lastname: Lucas
authors.forename: A M
authors.initials: AM
authors.lastname: Holburn
authors.forename: P W
authors.initials: PW
authors.lastname: Saunders
authors.forename: A H
authors.initials: AH
authors.lastname: Waters
mesh_terms: D000906:Antibodies / Q000032:analysis*; D001792:Blood Platelets / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005189:False Positive Reactions; D005455:Fluorescent Antibody Technique; D006680:HLA Antigens / Q000276:immunology; D006801:Humans; D007126:Immunoglobulin Allotypes / Q000032:analysis; D012015:Reference Standards; D011795:Surveys and Questionnaires
chemical_list: D000906:Antibodies; D006680:HLA Antigens; D007126:Immunoglobulin Allotypes