title: Injection of physiological saline facilitates recovery of ascitic fluids for monoclonal antibody production.
abstract: The intraperitoneal injection of physiological saline into anti-C-reactive protein (anti-CRP) hybridoma tumor-bearing mice facilitates extraction of diluted ascitic fluids. The dilution with physiological saline before withdrawal of ascitic fluid reduces the processing time in large scale operations, facilitates further manipulations, increases the number of extractions per animal and permits the recovery of ascitic fluid containing monoclonal antibodies from solid tumor-bearing mice.
authors.affiliation: Research Department, INVESGEN, Madrid, Spain.
authors.forename: J M
authors.initials: JM
authors.lastname: Coll
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D001201:Ascites / Q000276:immunology*; D002097:C-Reactive Protein / Q000276:immunology; D007158:Immunologic Techniques; D051379:Mice; D012965:Sodium Chloride / Q000494:pharmacology; D013997:Time Factors
chemical_list: D000911:Antibodies, Monoclonal; D012965:Sodium Chloride; D002097:C-Reactive Protein