title: Suppression of mutations conferring penicillin tolerance by interference with the stringent control mechanism of Escherichia coli.
abstract: Mutations in Escherichia coli previously reported (R. E. Harkness and E. E. Ishiguro, J. Bacteriol. 155:15-21, 1983; L. C. Shimmin, D. Vanderwel, R. E. Harkness, B. R. Currie, A. Galloway, and E. E. Ishiguro, J. Gen. Microbiol. 130:1315-1323, 1984) as conferring a temperature-dependent tolerance to lysis induced by inhibitors of peptidoglycan synthesis were suppressed by treatment with inhibitors of the stringent response or by introduction of a relA mutation. The relA+ derivatives of the mutants exhibited a stringent response at the nonpermissive temperature. The consequent inhibition of the autolytic enzyme system (W. Kusser and E. E. Ishiguro, J. Bacteriol. 164:861-865, 1985) was apparently responsible for the lysis-tolerant phenotypes of these mutants.
authors.forename: W
authors.initials: W
authors.lastname: Kusser
authors.forename: E E
authors.initials: EE
authors.lastname: Ishiguro
mesh_terms: D000667:Ampicillin / Q000494:pharmacology*; D001433:Bacteriolysis / Q000187:drug effects; D002701:Chloramphenicol / Q000494:pharmacology; D004361:Drug Tolerance; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics*; D005839:Gentamicins / Q000494:pharmacology; D007612:Kanamycin / Q000494:pharmacology; D010641:Phenotype; D013489:Suppression, Genetic; D013696:Temperature
chemical_list: D005839:Gentamicins; D007612:Kanamycin; D002701:Chloramphenicol; D000667:Ampicillin