Abortive infection of Escherichia coli F+ cells by bacteriophage T7 requires ribosomal misreading.
The use of different precisely mapped T3/T7 recombinants strengthens the conclusion that abortive infection by T7 of F plasmid-carrying cells is due to the nucleotide sequence at the end of the T7 gene 1. Furthermore, we demonstrate that the exclusion requires suppression of ochre stop codons, a phenomenon that occurs with low frequency in wild-type cells due to ribosomal misreading. The introduction of rspL mutations in which ribosomal misreading is reduced alleviates the exclusion and the presence of ochre tRNA suppressors increases its severity.
Krüger
D H
DH
Bickle
T A
TA
D003062:Codon; D004274:DNA, Recombinant; D004279:DNA, Viral; D004926:Escherichia coli / Q000235:genetics*; D005144:F Factor; D005814:Genes, Viral; D005815:Genetic Code; D013489:Suppression, Genetic; D013604:T-Phages / Q000235:genetics* / Q000502:physiology; D014779:Virus Replication
D003062:Codon; D004274:DNA, Recombinant; D004279:DNA, Viral