Biofilms on right heart flow-directed catheters.
This study was designed to detect biofilm and bacteria on right heart flow-directed catheters using scanning electron microscopy and culture following scraping and dispersion of biofilm by sonication. We examined 20 consecutive catheters removed from 18 critically ill patients, an average of 2.6 days after insertion. On scanning electron microscopy, all catheters were found to be covered by a biofilm, with bacteria visible on 50 percent of them. Cultures of specimens from 40 percent of the catheters grew skin organisms (Staphylococcus warneri, Diphtheroid), anaerobes (Propionibacterium), and other potential pathogens (Proteus vulgaris, Enterobacter cloacae). Combination of the two techniques produced a bacterial detection rate of 75 percent. This study demonstrates that the presence of biofilm with bacterial adherence is common on right heart flow-directed catheters. The phenomenon could play a significant role in endogenous infection in critically ill patients.
Passerini
L
L
Phang
P T
PT
Jackson
F L
FL
Lam
K
K
Costerton
J W
JW
King
E G
EG
D001419:Bacteria / Q000302:isolation & purification*; D001422:Bacterial Adhesion; D001424:Bacterial Infections / Q000209:etiology*; D001431:Bacteriological Techniques; D006328:Cardiac Catheterization / Q000009:adverse effects / Q000295:instrumentation*; D006801:Humans; D007362:Intensive Care Units; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D011651:Pulmonary Artery; D012306:Risk