title: Amino acid sequence of guinea pig prostate kallikrein.
abstract: The primary structure of the major arginine esteropeptidase from guinea pig prostate has been deduced from automated Edman degradation of peptides generated by clostripain, cyanogen bromide, endoproteinase Lys-C, and Staphylococcus aureus V8 protease digestion of the protein. The esteropeptidase is a single polypeptide chain comprised of 239 amino acids and contains 2 apparent sites of carbohydrate attachment, Asn-78 and Asn-169. Both occur in consensus sequences for N-linked glycosylation sites. The esteropeptidase exhibits approximately 35% homology with trypsin including conservation of the catalytic residues and the aspartic acid which confers specificity toward basic amino acids. The sequence identity, however, extends to greater than 60% with the kallikrein family of serine proteases. In addition to the overall homology, the guinea pig enzyme displays a number of features characteristic of kallikreins including 10 conserved half-cystine residues, a C-terminal proline, and the "kallikrein loop". On the basis of this structural relatedness, the enzyme has been designed as guinea pig prostate kallikrein. In contrast to many of the kallikreins of other species and tissues, this enzyme does not contain any sites within the kallikrein loop sensitive to proteases that result in internal breaks in the polypeptide chain.
authors.affiliation: Department of Biological Chemistry, California College of Medicine, University of California, Irvine 92717.
authors.forename: J C
authors.initials: JC
authors.lastname: Dunbar
authors.forename: R A
authors.initials: RA
authors.lastname: Bradshaw
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003488:Cyanogen Bromide; D006168:Guinea Pigs; D007461:Iodoacetates; D019807:Iodoacetic Acid; D007610:Kallikreins / Q000302:isolation & purification; D008297:Male; D010446:Peptide Fragments / Q000032:analysis; D010447:Peptide Hydrolases; D011467:Prostate / Q000201:enzymology*
chemical_list: D007461:Iodoacetates; D010446:Peptide Fragments; D010447:Peptide Hydrolases; D007610:Kallikreins; D003488:Cyanogen Bromide; D019807:Iodoacetic Acid