Photoreactivation in phr mutants of Escherichia coli K-12.
We have investigated the genetics of photoreactivation in Escherichia coli K-12. We found that strains with point mutations or deletions in the phr gene showed a significant residual level of photoreactivation after exposure to large fluences of photoreactivating light. It had been previously proposed that a gene in the gal-att lambda interval is also involved in photoreactivation and that the residual photoreactivating activity might be due to this so-called phrA gene located at this interval. We found that deletions of the gal-att lambda region had no effect on either the rate or the final extent of photoreactivation observed in phr+ cells or phr mutants; however strains carrying the delta (gal-att lambda) deletions displayed increased sensitivity to near-UV radiation.
Husain
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Sancar
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D001426:Bacterial Proteins / Q000235:genetics*; D002872:Chromosome Deletion; D002874:Chromosome Mapping; D004260:DNA Repair; D004255:Deoxyribodipyrimidine Photo-Lyase / Q000235:genetics*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics* / Q000528:radiation effects; D005420:Flavoproteins / Q000235:genetics*; D005798:Genes, Bacterial; D008190:Lyases / Q000235:genetics*; D009154:Mutation; D014466:Ultraviolet Rays
D001426:Bacterial Proteins; D005420:Flavoproteins; D008190:Lyases; D004255:Deoxyribodipyrimidine Photo-Lyase