Identification of oestrogen receptors in cells of paraffin-processed breast cancers by IGSS.
Oestrogen receptor (ER) analysis of breast cancers by the standard dextran coated charcoal (DCC) method and the oestrogen receptor immunocytochemical assay (ERICA), shows that ERICA is more sensitive. We find that the immunogold-silver staining technique (IGSS), which is used on paraffin sections, is applicable to the ERICA antibody and that the DCC and IGSS methods have comparable sensitivity. Reasons for wishing to develop an improved method for oestrogen receptor localisation in paraffin sections and its advantages are given.
Teasdale
J
J
Jackson
P
P
Holgate
C S
CS
Cowen
P N
PN
D001943:Breast Neoplasms / Q000032:analysis*; D002277:Carcinoma / Q000032:analysis*; D006046:Gold; D006652:Histological Techniques; D006801:Humans; D007158:Immunologic Techniques; D010232:Paraffin; D011960:Receptors, Estrogen / Q000032:analysis*; D012834:Silver
D011960:Receptors, Estrogen; D012834:Silver; D006046:Gold; D010232:Paraffin