ELISA detectable virulence marker antigen of enteroinvasive Escherichia coli is coded by a 140 megadalton plasmid.
The ELISA reactive virulence marker antigen, characteristic of enteroinvasive Escherichia coli and virulent Shigella, expressed in an E. coli K-12 recipient after the 140 Md plasmid of an enteroinvasive E. coli strain had been introduced into it. Similarly to the epithelial cell penetration and Congo red binding capacities--known to be coded by the above plasmid--the virulence marker antigen expressed also only at 37 degrees C but not at 30 degrees C. These data suggest the plasmid coded nature of the virulence marker antigen.
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D000942:Antigens, Bacterial / Q000032:analysis / Q000235:genetics*; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000235:genetics* / Q000276:immunology / Q000472:pathogenicity; D010957:Plasmids; D014774:Virulence
D000942:Antigens, Bacterial; D004269:DNA, Bacterial