Human sperm carbohydrate antigens defined by an antisperm human monoclonal antibody derived from an infertile woman bearing antisperm antibodies in her serum.
The epitope structure of the human sperm antigen reacting with antibodies present in sera of infertile women has been studied using mAb H6-3C4, which produces immobilization of human sperm in the presence of complement. Another antibody, NUH2, which also induces human sperm immobilization, was used to substantiate the presence of a receptor on sperm involved in susceptibility to immobilization. Both antibodies defined type 2 chain polylactosamine structure. H6-3C4 is directed to internally located repetitive N-acetyllactosamine, i.e., sialyl-i, i, or fucosyl-i. NUH2 defines binary alpha 2----3 sialyl type 2 chain, i.e., sialyl-I. Thus, the presence of antibodies in the sera of infertile women directed to sperm lactosaminoglycan or lactosaminolipid could be the basis for infertility in these cases.
Tsuji
Y
Y
Biomembrane Institute, University of Washington, Seattle 98119.
Clausen
H
H
Nudelman
E
E
Kaizu
T
T
Hakomori
S
S
Isojima
S
S
D000606:Amino Sugars / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D000941:Antigens / Q000276:immunology*; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000276:immunology*; D002855:Chromatography, Thin Layer; D003165:Complement System Proteins / Q000276:immunology; D005260:Female; D005455:Fluorescent Antibody Technique; D006384:Hemagglutination; D006801:Humans; D007118:Immunoassay; D007247:Infertility, Female / Q000276:immunology*; D008297:Male; D008969:Molecular Sequence Data; D011863:Radioimmunoassay; D013081:Sperm Motility; D013094:Spermatozoa / Q000276:immunology*
D000606:Amino Sugars; D000911:Antibodies, Monoclonal; D000941:Antigens; D002241:Carbohydrates; C000458:N-acetyllactosamine; D003165:Complement System Proteins