[Comparison of two ELISA kits for the demonstration of antibodies against LAV/HTLV-III].
Eight hundred sera from a non-risk group and thousand sera from people at risk for acquiring AIDS were tested for the presence of LAV/HTLV-III specific antibodies by ELISA test kits manufactured by Du Pont and Organon. Western Blot analysis was used as a confirmatory test. All Western Blot positive sera were also positive in the Du Pont ELISA which in addition revealed a very low rate of false positive results (0.44%). In the Organon ELISA negative results were obtained with one positive and one questionable positive serum, as determined by Western Blot. The possible problem of test sensitivity being too low has been taken into account by the manufacturer by changing the calculation of cut-off values.
Heinz
F X
FX
Kunz
C
C
Barrett
N
N
D000163:Acquired Immunodeficiency Syndrome / Q000175:diagnosis*; D000914:Antibodies, Viral / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D005189:False Positive Reactions; D005455:Fluorescent Antibody Technique; D006678:HIV / Q000276:immunology*; D015483:HIV Antibodies; D006801:Humans; D007158:Immunologic Techniques; D011237:Predictive Value of Tests; D011933:Reagent Kits, Diagnostic; D012306:Risk
D000914:Antibodies, Viral; D015483:HIV Antibodies; D011933:Reagent Kits, Diagnostic
Vergleich von zwei ELISA-Kits zum Nachweis von Antikörpern gegen LAV/HTLV-III.