Immunophenotyping of non-Hodgkin's lymphomas using a panel of antibodies on paraffin-embedded tissues.
The use of monoclonal and polyclonal antibodies for the immunophenotyping of non-Hodgkin's lymphomas in paraffin-embedded tissue has been limited by the fact that most antigens on lymphoid cells are denatured by histologic fixation, dehydration, and embedment. In this article the authors have analyzed a small panel of antibodies which represent exceptions to this rule, in that they identify denaturation-resistant determinants on leukocyte antigens in paraffin-embedded tissue. Monoclonal antibodies L26 [corrected] and 4KB5 label preferentially B cells, monoclonal antibody UCHL1 stains predominantly T cells, and monoclonal antibody MAC 387 reacts with granulocytes and some macrophages. A polyclonal antiserum raised against purified CD3 (T3) antigen, a T-cell-specific molecule, was also employed. This antibody panel was used to immunophenotype routinely processed tissue biopsy specimens from 61 non-Hodgkin's lymphomas (all of which had been previously phenotyped in cryostat sections). The lineage of the neoplastic cells was correctly identified in 32 of 34 (94%) cases of B-cell lymphoma, in 19 of 19 (100%) cases of T-cell neoplasm, and in 2 of 4 (50%) cases of histiocytic malignancy. It is concluded that this combination of antibodies is helpful in immunophenotyping non-Hodgkin's lymphomas when only paraffin-embedded tissue sections are available, although additional reagents of higher specificity are required to improve the identification of lymphomas.
Davey
F R
FR
SUNY Health Science Center at Syracuse 13210.
Gatter
K C
KC
Ralfkiaer
E
E
Pulford
K A
KA
Krissansen
G W
GW
Mason
D Y
DY
D000911:Antibodies, Monoclonal; D001402:B-Lymphocytes / Q000276:immunology; D014408:Biomarkers, Tumor / Q000032:analysis; D005404:Fixatives; D006801:Humans; D007124:Immunoenzyme Techniques; D008228:Lymphoma, Non-Hodgkin / Q000276:immunology / Q000473:pathology*; D008264:Macrophages / Q000276:immunology; D010232:Paraffin; D010641:Phenotype; D013601:T-Lymphocytes / Q000276:immunology
D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; D005404:Fixatives; D010232:Paraffin