Purification and DNA-binding properties of FIS and Cin, two proteins required for the bacteriophage P1 site-specific recombination system, cin.
An Escherichia coli chromosomally coded factor termed FIS (Factor for Inversion Stimulation) stimulates the Cin protein-mediated, site-specific DNA inversion system of bacteriophage P1 more than 500-fold. We have purified FIS and the recombinase Cin, and studied the inversion reaction in vitro. DNA footprinting studies with DNase I showed that Cin specifically binds to the recombination site, called cix. FIS does not bind to cix sites but does bind to a recombinational enhancer sequence that is required in cis for efficient recombination. FIS also binds specifically to sequences outside the enhancer, as well as to sequences unrelated to Cin inversion. On the basis of these data, we discuss the possibility of additional functions for FIS in E. coli.
Haffter
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Department of Microbiology, University of Basel, Switzerland.
Bickle
T A
TA
D001483:Base Sequence; D002352:Carrier Proteins / Q000302:isolation & purification*; D003090:Coliphages / Q000235:genetics*; D004254:DNA Nucleotidyltransferases / Q000302:isolation & purification*; D004279:DNA, Viral / Q000378:metabolism*; D004742:Enhancer Elements, Genetic; D004926:Escherichia coli / Q000235:genetics; D029968:Escherichia coli Proteins; D035021:Factor For Inversion Stimulation Protein; D034982:Integration Host Factors; D011995:Recombination, Genetic
D002352:Carrier Proteins; D004279:DNA, Viral; D029968:Escherichia coli Proteins; D035021:Factor For Inversion Stimulation Protein; D034982:Integration Host Factors; C440502:integration host factor, E coli; D004254:DNA Nucleotidyltransferases; C039930:cin recombinase