Progenitor cell assays predict hematopoietic reconstitution after syngeneic transplantation in mice.
Hematopoietic reconstitution following syngeneic bone marrow transplantation with graded doses of untreated and drug-treated bone marrow was studied in B6D2F1 mice. Granulocyte-macrophage colony-forming units (CFU-GM) and spleen colony-forming units (CFU-S) showed similar in vitro drug sensitivities. Both the speed of hematologic recovery and survival of mice transplanted with untreated or drug-treated bone marrow were directly related to the number of CFU-GM or CFU-S transplanted. Similar hematologic recovery was seen for untreated marrow transplants and treated transplants that had similar CFU-GM or CFU-S content. There is a minimum number of transplanted CFU-GM or CFU-S that allows survival of lethally irradiated mice. This number is present in a marrow transplant containing the equivalent of 5 X 10(3) untreated cells or producing one to two spleen colonies. There also exists a maximum value for the number of hematopoietic progenitors in a marrow graft, above which the rate of hematologic recovery following transplantation is rapid and no detectable increase in the rate is seen with increasing CFU-GM or CFU-S content. The presence of this maximum value for transplanted progenitors and variations in culture techniques are probably the reasons previous studies have not always shown a correlation between CFU-GM content and hematologic recovery after bone marrow transplantation.
Jones
R J
RJ
Johns Hopkins Oncology Center, Johns Hopkins Medical Institutions, Baltimore, MD 21205.
Sharkis
S J
SJ
Celano
P
P
Colvin
O M
OM
Rowley
S D
SD
Sensenbrenner
L L
LL
D000818:Animals; D001853:Bone Marrow / Q000502:physiology; D016026:Bone Marrow Transplantation; D003114:Colony-Forming Units Assay; D005260:Female; D005544:Forecasting; D006410:Hematopoiesis; D007700:Kinetics; D051379:Mice; D008815:Mice, Inbred Strains; D014185:Transplantation, Isogeneic