title: Improvement of cytotoxicity of tumor necrosis factor (TNF) by increase in basicity of its N-terminal region.
abstract: Two new recombinant TNFs (named rTNF-Scw1 and -Scw2) with higher basicity than conventional recombinant human TNF-alpha (rTNF-alpha) in the N-terminal region were constructed. Their sequences were constructed based on those of partially purified cytotoxic factors from the culture supernatant of acute monocytic leukemia cells THP-1, which unlike rTNF-alpha are cytotoxic to T24 bladder carcinoma cells in vitro. These new rTNF-Ss showed a broader cytotoxicity to tumor cells than rTNF-alpha. This increase in the basicity of the N-terminal region over that of conventional TNF significantly increased the cytotoxicity on tumor cells in vivo as well as in vitro.
authors.affiliation: Biotechnology Research Center, Teikyo University, Kanagawa, Japan.
authors.forename: G
authors.initials: G
authors.lastname: Soma
authors.forename: N
authors.initials: N
authors.lastname: Kitahara
authors.forename: Y
authors.initials: Y
authors.lastname: Tsuji
authors.forename: M
authors.initials: M
authors.lastname: Kato
authors.forename: H
authors.initials: H
authors.lastname: Oshima
authors.forename: T
authors.initials: T
authors.lastname: Gatanaga
authors.forename: H
authors.initials: H
authors.lastname: Inagawa
authors.forename: K
authors.initials: K
authors.lastname: Noguchi
authors.forename: Y
authors.initials: Y
authors.lastname: Tanabe
authors.forename: D
authors.initials: D
authors.lastname: Mizuno
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D007739:L Cells / Q000166:cytology / Q000187:drug effects; D051379:Mice; D008969:Molecular Sequence Data; D010957:Plasmids; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000633:toxicity*; D013329:Structure-Activity Relationship; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000302:isolation & purification / Q000633:toxicity*
chemical_list: D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha