title: Transcriptional regulation of the DAL5 gene in Saccharomyces cerevisiae.
abstract: We demonstrate that the DAL5 gene, encoding a necessary component of the allantoate transport system, is constitutively expressed in Saccharomyces cerevisiae. Its relatively high basal level of expression did not increase further upon addition of allantoin pathway intermediates. However, steady-state DAL5 mRNA levels dropped precipitously when a repressive nitrogen source was provided. These control characteristics of DAL5 expression make this gene a good model with which to unravel the mechanism of nitrogen catabolite repression. Its particular advantage relative to other potentially useful genes derives from its lack of control by induction and hence the complicating effects of inducer exclusion.
authors.forename: R
authors.initials: R
authors.lastname: Rai
authors.forename: F
authors.initials: F
authors.lastname: Genbauffe
authors.forename: H Z
authors.initials: HZ
authors.lastname: Lea
authors.forename: T G
authors.initials: TG
authors.lastname: Cooper
mesh_terms: D000481:Allantoin / Q000378:metabolism; D001693:Biological Transport, Active; D005786:Gene Expression Regulation; D005800:Genes, Fungal; D005816:Genetic Complementation Test; D010072:Oxamic Acid / Q000031:analogs & derivatives / Q000378:metabolism; D011392:Proline / Q000378:metabolism; D012331:RNA, Fungal / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic; D014508:Urea / Q000031:analogs & derivatives* / Q000378:metabolism
chemical_list: D012331:RNA, Fungal; D012333:RNA, Messenger; C024081:allantoic acid; D000481:Allantoin; C008727:oxaluric acid; D014508:Urea; D011392:Proline; D010072:Oxamic Acid