Visualization of ouabain binding in bovine cornea.
In these experiments, the binding of ouabain, a specific inhibitor of NA,K-ATPase is visualized in bovine cornea using an immunohistochemical method. Due to cross-reactivity between ouabain and digitoxin antiserum, the ouabain binding is demonstrated after an immunological labelling with fluorescein. As shown in the corneal tissue sections and in the epithelial and endothelial cell preparations, a bright fluorescence of the endothelial cell membranes is observed in corneae incubated in 10(-5)-10(-3) mol/l ouabain. In the epithelium, only a weak binding of the ouabain is observed at the basolateral membrane after incubation in 10(-3) mol/l ouabain.
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Department of Ophthalmology, Faculty of Medicine, University of Trondheim, Norway.
D000818:Animals; D001665:Binding Sites; D002417:Cattle; D003315:Cornea / Q000166:cytology / Q000378:metabolism*; D004074:Digitoxin / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D010042:Ouabain / Q000378:metabolism*
D010042:Ouabain; D004074:Digitoxin