title: Determination of polyamines and metabolites in cerebrospinal fluid by isotope dilution mass fragmentography, and a clinical application.
abstract: Capillary gas chromatography and mass fragmentography was used to determine simultaneously 1,3-diaminopropane, putrescine, cadaverine, spermidine, spermine, isoputreanine and putreanine in cerebrospinal fluid. After addition of deuterium labelled analogs and acid hydrolysis, the compounds were isolated by adsorption onto silica and converted into their N-heptafluorobutyryl-methylesters. Quality control data and an application of the method are given. A patient harbouring an astrocytoma was monitored during chemotherapeutic treatment.
authors.affiliation: Central Laboratory for Clinical Chemistry, University Hospital, Groningen, The Netherlands.
authors.forename: G A
authors.initials: GA
authors.lastname: van den Berg
authors.forename: J M
authors.initials: JM
authors.lastname: Schaaf
authors.forename: G T
authors.initials: GT
authors.lastname: Nagel
authors.forename: A W
authors.initials: AW
authors.lastname: Teelken
authors.forename: F A
authors.initials: FA
authors.lastname: Muskiet
mesh_terms: D001254:Astrocytoma / Q000134:cerebrospinal fluid; D001932:Brain Neoplasms / Q000134:cerebrospinal fluid; D002849:Chromatography, Gas; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007201:Indicator Dilution Techniques; D011073:Polyamines / Q000134:cerebrospinal fluid*
chemical_list: D011073:Polyamines