Autocrine stimulation of interleukin 1 beta in acute myelogenous leukemia cells.
A significant increase in CD25 antigen-positive cells by IL-1 was observed in cells of a patient with M7 acute myelogenous leukemia. Basal proliferation and expression of CD25 antigen by the M7 leukemic cells were inhibited by addition of anti-IL-1 beta antibody in a dose-dependent manner, but not by rabbit anti-IL-1 alpha antibody. Culture supernatants of these leukemic cells contained IL-1 activity, which was specifically inhibited by addition of anti-IL-1 beta antibody, and Northern blot analysis detected intracellular IL-1 beta mRNA. These results indicated that autocrine secretion of IL-1 beta was involved in proliferation of some myelogenous leukemic cells.
Sakai
K
K
Second Department of Internal Medicine, Kumamoto University Medical School, Japan.
Hattori
T
T
Matsuoka
M
M
Asou
N
N
Yamamoto
S
S
Sagawa
K
K
Takatsuki
K
K
D000954:Antigens, Surface / Q000032:analysis / Q000235:genetics; D002455:Cell Division; D005786:Gene Expression Regulation; D006801:Humans; D007158:Immunologic Techniques; D007375:Interleukin-1 / Q000235:genetics / Q000502:physiology*; D015470:Leukemia, Myeloid, Acute / Q000473:pathology* / Q000503:physiopathology; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000235:genetics; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
D000954:Antigens, Surface; D007375:Interleukin-1; D012333:RNA, Messenger