A yeast gene coding for a putative protein kinase homologous to cdc25 suppressing protein kinase.
A yeast gene termed YKR coding for a putative protein kinase was isolated by using the cloned cDNA for rabbit protein kinase C as a hybridization probe. The encoded protein (YKR), composed of 380 amino acid residues, shows extensive sequence homology to serine/threonine-specific protein kinases from various species in the approx. 320 C-terminal amino acid residues, strongly suggesting that YKR is endowed with a protein kinase activity. The observed homologies to the cdc25 suppressing protein kinase from yeast, the catalytic subunit of mammalian cAMP-dependent protein kinase, and mammalian protein kinase C were 76, 48 and 37%, respectively. Gene replacement experiments showed that YKR itself is not essential for cell proliferation.
Ohno
S
S
Department of Molecular Biology, Tokyo Metropolitan Institute of Medical Science, Japan.
Aoshima
M
M
Matsumoto
S
S
Yahara
I
I
Suzuki
K
K
D000595:Amino Acid Sequence; D000818:Animals; D004247:DNA / Q000032:analysis; D005796:Genes; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D011493:Protein Kinase C / Q000235:genetics; D011494:Protein Kinases / Q000235:genetics*; D011817:Rabbits; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D013489:Suppression, Genetic
D004247:DNA; D011494:Protein Kinases; D011493:Protein Kinase C