Should nagarse be used during the isolation of brain mitochondria?
When nagarse is used to isolate brain mitochondria, a proportion of the nagarse stays associated with the mitochondrial fraction. This results in no detrimental affect on the respiratory activities. The nagarse is active in the presence of 2.3% sodium dodecyl sulfate and when samples are prepared for sodium dodecyl sulfate-polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate, the nagarse degrades a substantial amount of the mitochondrial proteins.
Wilson
E J
EJ
Department of Clinical Neurological Sciences, University of Western Ontario, Victoria Hospital, London, Canada.
D000818:Animals; D001921:Brain / Q000648:ultrastructure*; D001923:Brain Chemistry / Q000187:drug effects; D002458:Cell Fractionation / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D008928:Mitochondria / Q000032:analysis / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000032:analysis; D010101:Oxygen Consumption / Q000187:drug effects; D051381:Rats; D013381:Subtilisins / Q000494:pharmacology
D009419:Nerve Tissue Proteins; D013381:Subtilisins