title: Overproduction and preliminary X-ray characterization of aspartate aminotransferase from Escherichia coli.
abstract: The aspartate aminotransferase of Escherichia coli was overproduced in cells after genetic manipulation, and was crystallized from a polyethylene glycol solution, pH 7.0. The crystals obtained were of good quality and had diffractions extending beyond 2.4 A. The space group and unit cell dimensions were determined with a precession camera and a four-circle diffractometer to be C222(1), and a = 157.1 A, b = 85.5 A, and c = 79.7 A, respectively. Only one protein subunit is contained in an asymmetric unit.
authors.forename: S
authors.initials: S
authors.lastname: Kamitori
authors.forename: K
authors.initials: K
authors.lastname: Hirotsu
authors.forename: T
authors.initials: T
authors.lastname: Higuchi
authors.forename: K
authors.initials: K
authors.lastname: Kondo
authors.forename: K
authors.initials: K
authors.lastname: Inoue
authors.forename: S
authors.initials: S
authors.lastname: Kuramitsu
authors.forename: H
authors.initials: H
authors.lastname: Kagamiyama
authors.forename: Y
authors.initials: Y
authors.lastname: Higuchi
authors.forename: N
authors.initials: N
authors.lastname: Yasuoka
authors.forename: M
authors.initials: M
authors.lastname: Kusunoki
mesh_terms: D001219:Aspartate Aminotransferases / Q000096:biosynthesis; D003460:Crystallization; D004926:Escherichia coli / Q000201:enzymology*; D014961:X-Ray Diffraction
chemical_list: D001219:Aspartate Aminotransferases