General strategies in the separation of proteins by high-performance liquid chromatographic methods.
General fractionation strategies for the high-resolution purification of proteins are described. The impact of different separation parameters and resolution optimisation approaches with tandem-based systems on retention and recovery behaviour is reviewed. Procedures for the successful linkage of different chromatographic steps into a preferred sequence of operations are discussed in terms of the underlying principles and modus operandi of high-performance liquid chromatographic purification of proteins and related biomacromolecules.
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D000818:Animals; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D011506:Proteins / Q000302:isolation & purification*
D011506:Proteins