Conditional absence of mitosis-specific antigens in a temperature-sensitive embryonic-arrest mutant of Caenorhabditis elegans.
A monoclonal antibody, specific to phosphoproteins in mitotic HeLa cells was found to crossreact with a similar set of proteins in embryos of the nematode, Caenorhabditis elegans. In C. elegans, as in mammalian cells, the highly conserved antigenic epitope is associated with a family of high molecular weight polypeptides. The antigenic reactivity of these multiple proteins also depends on their phosphorylation, since antibody binding is reduced after alkaline phosphatase treatment. The antigens are detected at the centrosomes, and in the nuclear region and surrounding cytoplasm of mitotic cells. The significance of these antigens is emphasized by their absence at restrictive temperature in embryos of the temperature-sensitive embryonic-arrest mutant, emb-29V. Furthermore, temperature shift-down experiments suggest that the emb-29 mutation defines a cell division cycle function that affects an essential activity required for progression into M phase.
Hecht
R M
RM
Department of Biochemical and Biophysical Sciences, University of Houston, TX 77004.
Berg-Zabelshansky
M
M
Rao
P N
PN
Davis
F M
FM
D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis*; D002107:Caenorhabditis / Q000032:analysis / Q000196:embryology / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D004625:Embryo, Nonmammalian / Q000032:analysis; D005455:Fluorescent Antibody Technique; D006358:Hot Temperature; D008938:Mitosis; D009154:Mutation; D010750:Phosphoproteins / Q000032:analysis*
D000947:Antigens, Helminth; D010750:Phosphoproteins