[Immunoenzyme demonstration of viruses and virus-specific antibodies by using conjugates based on gene engineering-produced beta-lactamase].
Enzyme immunoassays for the detection of viral antigens and virus-specific antibodies in biological samples have been described. Molecular complexes of antibodies and beta-lactamase (penicillinase) have been used as anti-specific conjugates. To synthesize the conjugate, the enzyme obtained with the aid of genetic engineering has been used. Enzyme immunoassays have been tested for the indication of the influenza virus and virus-induced specific antibodies. Enzyme immunoassays were shown to possess certain advantages (e.g., the use of simple and nontoxic substrate) along with the sensitivity identical to that of other methods, employing peroxidase-based conjugates.
Kharitonenkov
I G
IG
Kordium
V A
VA
Khristova
M L
ML
Leonov
S V
SV
Kirillova
V S
VS
D000914:Antibodies, Viral / Q000032:analysis*; D000918:Antibody Specificity; D005069:Evaluation Studies as Topic; D005818:Genetic Engineering; D007124:Immunoenzyme Techniques; D009980:Influenza A virus / Q000276:immunology / Q000302:isolation & purification*; D001618:beta-Lactamases
D000914:Antibodies, Viral; D001618:beta-Lactamases
Immunofermentnaia indikatsiia virusov i virusspetsificheskikh antitel s isol'zovaniem kon''iugatov na osnove beta-laktamazy, poluchennoĭ genno-inzhenernym sposobom.