Immunologic memory responses induced in BALB/c mice by cross-linked outer membrane extracts of four Salmonella serotypes.
Outer membrane proteins (OMP), extracted from Salmonella enteritidis, S anatum, S typhimurium, and S infantis, were cross-linked to form a large immunogen (4-OMP-lipopolysaccharide [LPS]). Vaccinations with 4-OMP-LPS dissolved in phosphate-buffered saline solution and 4-OMP-LPS emulsified with muramyl dipeptide were capable of eliciting specific and sustained primary IgM and IgG responses in BALB/c mice, as well as inducing immunologic memory for 130 days. In addition to 4-OMP-LPS-specific responses, substantial IgM and IgG responses specific for each live homologous organism were detected over the 130-day trial. In comparison with vaccination with 4-OMP-LPS dissolved in phosphate-buffered saline solution, responses specific for the antigen or the homologous Salmonella were not markedly increased in mice vaccinated with 4-OMP-LPS emulsified with muramyl dipeptide. Seemingly, cross-linked OMP, without the inclusion of muramyl dipeptide, may have potential as vaccine components and may induce immunologic memory.
Kudrna
D A
DA
Teresa
G W
GW
Arnzen
J M
JM
Beard
K S
KS
Olson
D
D
D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D007074:Immunoglobulin G / Q000276:immunology*; D007075:Immunoglobulin M / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D012475:Salmonella / Q000276:immunology*; D012477:Salmonella enteritidis / Q000276:immunology; D012486:Salmonella typhimurium / Q000276:immunology
D001425:Bacterial Outer Membrane Proteins; D007074:Immunoglobulin G; D007075:Immunoglobulin M