title: Interaction of positive and negative regulatory proteins in the galactose regulon of yeast.
abstract: Transcriptional regulation in the galactose regulon of yeast is determined by an interplay between a positive regulatory protein, GAL4, and a negative regulatory protein, GAL80. We show that derivatives of GAL4 missing as few as 28 carboxy-terminal amino acids are not responsive to GAL80 regulation, implying that the carboxyl terminus of GAL4 is required for interaction with GAL80. Furthermore, a lesion in GAL4 that genetically defines the GAL80-interactive region maps to the 3' end of the gene. Since the carboxyl terminus of GAL4 has also been implicated in interaction with a transcriptional factor, we propose a model for negative regulation in which GAL80 and this factor compete for binding a common region of GAL4, and the on/off state of transcription is determined by the relative affinities of the negative regulator and the transcriptional factor for this region.
authors.forename: S A
authors.initials: SA
authors.lastname: Johnston
authors.forename: J M
authors.initials: JM
authors.lastname: Salmeron
authors.forename: S S
authors.initials: SS
authors.lastname: Dincher
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D004271:DNA, Fungal / Q000235:genetics; D005690:Galactose / Q000235:genetics*; D005786:Gene Expression Regulation; D005800:Genes, Fungal; D012097:Repressor Proteins / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D013329:Structure-Activity Relationship; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D004271:DNA, Fungal; D012097:Repressor Proteins; D014157:Transcription Factors; D005690:Galactose