Microbore HPLC/mass spectrometry for the analysis of peptide mixtures using a continuous flow interface.
Microbore HPLC techniques have been combined with fast atom bombardment mass spectrometry to provide HPLC/MS capabilities for the analysis of mixtures of peptides and small proteins. The interface between the liquid chromatograph and mass spectrometer is a continuous flow direct insertion probe which contains a fused silica capillary that delivers the eluting solvent to the FAB source of the mass spectrometer at a rate of 5-10 microL/min. Data are presented for the analysis of several mixtures of peptides ranging in molecular weights from about 900 to 6000 daltons. In addition, the analysis of 100 pmol of a tryptic digest of whale myoglobin is shown where 16 of the possible 19 peptides were identified in the mass range m/z 2200-250. The advantages of this approach to HPLC/MS are a relatively high sensitivity because of the low flow rates and low background, and the ability to detect high molecular weight compounds.
Caprioli
R M
RM
DaGue
B
B
Fan
T
T
Moore
W T
WT
D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D007328:Insulin / Q000032:analysis; D013058:Mass Spectrometry; D008722:Methods; D009211:Myoglobin / Q000032:analysis; D010455:Peptides / Q000032:analysis*; D012756:Sheep; D013552:Swine; D014357:Trypsin / Q000378:metabolism; D014907:Whales
D007328:Insulin; D009211:Myoglobin; D010455:Peptides; D014357:Trypsin