Transformation of Aspergillus nidulans with the hygromycin-resistance gene, hph.
Aspergillus nidulans strain G191 was transformed to hygromycin resistance using plasmid pDH25, which contains the bacterial hygromycin B phosphotransferase gene (hph) fused to promoter elements of the A. nidulans trpC gene. Southern hybridizations of transformants revealed multiple, integrated copies of the vector. A pleiotropic effect conferring increased hygromycin B sensitivity was found to be associated with the A. nidulans pyrG89 allele. Plasmid pDH25 features a ClaI site immediately preceding the hph start codon thus permitting convenient replacement of the trpC sequences with other eukaryotic promoters.
Cullen
D
D
Forest Products Laboratory, USDA, Madison, WI 53705.
Leong
S A
SA
Wilson
L J
LJ
Henner
D J
DJ
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001233:Aspergillus nidulans / Q000187:drug effects / Q000235:genetics*; D001483:Base Sequence; D004352:Drug Resistance, Microbial; D005800:Genes, Fungal; D005838:Genotype; D006921:Hygromycin B / Q000494:pharmacology*; D010957:Plasmids; D014170:Transformation, Genetic
D000900:Anti-Bacterial Agents; D006921:Hygromycin B