title: Automatic nitrous oxide synchronization of mitotic human cell cultures.
abstract: Large numbers of human cells can be reversibly arrested in mitosis by high-pressure nitrous oxide. The optimum schedule for this arrest requires that the high-pressure block be started around midnight to provide a mitotic population that can be released the next morning to progress in synchrony through the next cell cycle. We describe a simple and safe device which can be set up at the end of the day and automatically exposes the cells to high-pressure nitrous oxide overnight.
authors.affiliation: University of Cambridge, Department of Zoology, United Kingdom.
authors.forename: C S
authors.initials: CS
authors.lastname: Downes
authors.forename: D M
authors.initials: DM
authors.lastname: Unwin
authors.forename: R G
authors.initials: RG
authors.lastname: Northfield
authors.forename: M J
authors.initials: MJ
authors.lastname: Berry
mesh_terms: D002478:Cells, Cultured; D003584:Cytological Techniques / Q000295:instrumentation; D006801:Humans; D008938:Mitosis / Q000187:drug effects*; D009609:Nitrous Oxide
chemical_list: D009609:Nitrous Oxide