[Early detection and rapid identification of Streptococcus pneumoniae and Haemophilus influenzae in acute pneumonias].
The comparative study of the diagnostic value of the enzyme immunoassay (EIA), indirect immunofluorescence (IF) and countercurrent immunoelectrophoresis (CIE) was made. The serological identification of the isolated and reference pneumococci (19) and H. influenzae (38) strains revealed the possibility of using all three microanalytical methods for this purpose. The study of pneumococcal and H. influenzae antigens in native sputum obtained from 74 patients with acute pneumonia showed that EIA and indirect IF were highly sensitive, their sensitivity considerably exceeding that of the bacteriological analysis. Pneumococcal antigens were detected in 66.2% of patients by EIA and in 54.0% of patients by indirect IF, while H. influenzae antigens were detected in 58.1% of patients by EIA and in 67.6% of patients by indirect IF. The sensitivity of CIE proved to be considerably lower; in the detection of pneumococcal antigens it was level with the sensitivity of the bacteriological analysis (23.0%) and H. influenzae antigens could be detected only in 27.0% of patients.
Frolov
A F
AF
Rybalko
S L
SL
Shapiro
A V
AV
Ivanskaia
N V
NV
Vasina
A G
AG
D000208:Acute Disease; D000907:Antibodies, Bacterial / Q000032:analysis*; D000942:Antigens, Bacterial / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D006192:Haemophilus Infections / Q000175:diagnosis* / Q000382:microbiology; D006193:Haemophilus influenzae / Q000145:classification / Q000276:immunology* / Q000302:isolation & purification; D006801:Humans; D007122:Immunoelectrophoresis; D007124:Immunoenzyme Techniques; D011014:Pneumonia / Q000175:diagnosis* / Q000382:microbiology; D011018:Pneumonia, Pneumococcal / Q000175:diagnosis* / Q000382:microbiology; D012703:Serotyping; D013183:Sputum / Q000382:microbiology; D013296:Streptococcus pneumoniae / Q000145:classification / Q000276:immunology* / Q000302:isolation & purification
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial
Rannee obnaruzhenie i bystraia identifikatsiia Streptococcus pneumoniae i Haemophilus influenzae pri ostrykh pnevmoniiakh.