Relationship between proteins encoded by three human gamma-crystallin genes and distinct polypeptides in the eye lens.
Although individual gamma-crystallins from the human eye lens have not been successfully purified and sequenced, most of the genes coding for these lens-specific structural proteins have been cloned and characterized. To investigate the relationship between these genes and the gamma-crystallins of the human lens, we made use of mouse cell lines which contain stably integrated copies of the coding sequences for three of the human gamma-crystallin genes coupled to the human metallothionein IIA promoter. The proteins produced by these hybrid genes in cell culture were detected immunologically and compared by physical characteristics with the gamma-crystallins from the human lens. The protein encoded by the G3 gene showed properties identical to those of the 21,000-molecular-weight gamma-crystallin from 11-month-old lens. The protein isolated from the cells expressing the G4 gene was similar to a 19,000-molecular-weight lens gamma-crystallin, while gene G5 encodes a highly basic gamma-crystallin which may be synthesized in only limited amounts in the human lens. These correlations provide a basis for future investigations on the relationship between putative mutations in human gamma-crystallin genes and altered proteins in hereditary lens cataracts.
Russell
P
P
Laboratory of Mechanisms of Ocular Disease, National Eye Institute, Bethesda, Maryland 20892.
Meakin
S O
SO
Hohman
T C
TC
Tsui
L C
LC
Breitman
M L
ML
D000818:Animals; D002460:Cell Line; D003459:Crystallins / Q000235:genetics*; D005796:Genes; D006801:Humans; D007163:Immunosorbent Techniques; D007526:Isoelectric Point; D007908:Lens, Crystalline / Q000502:physiology*; D051379:Mice; D008970:Molecular Weight; D005810:Multigene Family; D014162:Transfection
D003459:Crystallins