title: Murine monoclonal antibody against aldosterone: production, characterization and use for enzymoimmunoassay.
abstract: Hybridomas secreting monoclonal antibodies to aldosterone were obtained by fusion of myeloma cells and spleen cells from Balb/c mice immunized with aldosterone-3-carboxylmethyloxime-bovine serum albumin. A monoclonal antibody was purified from ascites fluid and characterized. An affinity constant of 1.61 x 10(9) M-1 has been measured and no cross-reactivity with tetrahydroaldosterone (THA), cortisol, cortisone, corticosterone, deoxycorticosterone (DOC), dehydroepiandrosterone (DHA), progesterone and estrone, could be detected. A peroxidase conjugated-antibody (1.5 mole of enzyme per mole of antibody) was obtained and used for microwell enzyme immunoassay and Immun-Blot assay. The high affinity and specificity of this antibody should make the direct determination of aldosterone in biological fluids possible at concentrations as low as 5 x 10(-10) M.
authors.affiliation: Unité Associée 586 du Centre National de la Recherche Scientifique, Faculté de Médecine, Paris, France.
authors.forename: S
authors.initials: S
authors.lastname: de Lauzon
authors.forename: N
authors.initials: N
authors.lastname: Le Trang
authors.forename: M F
authors.initials: MF
authors.lastname: Moreau
authors.forename: M
authors.initials: M
authors.lastname: Gentin
authors.forename: N
authors.initials: N
authors.lastname: Christeff
authors.forename: B
authors.initials: B
authors.lastname: Desfosses
authors.forename: N
authors.initials: N
authors.lastname: Cittanova
mesh_terms: D000450:Aldosterone / Q000032:analysis* / Q000276:immunology; D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification; D005260:Female; D006825:Hybridomas / Q000276:immunology; D007124:Immunoenzyme Techniques; D007202:Indicators and Reagents; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011863:Radioimmunoassay / Q000379:methods
chemical_list: D000911:Antibodies, Monoclonal; D007202:Indicators and Reagents; D000450:Aldosterone