Study of canine parvovirus polypeptides by immunoblot analysis.
The immunoblot analysis of canine parvovirus (CPV) polypeptides with 47 rat monoclonal antibodies (RH) has revealed four different types of reaction. Many of these antibodies do not recognize the electroblotted proteins. However, among these monoclonals that do react, 12 recognize all three viral polypeptides (VP67, VP70 and VP85), 3 recognize preferentially the VP85 and one is exclusively directed against VP70. Thus, three antigenic regions are proposed which correspond to domains of the CPV polypeptides. In addition, these results together with evidence for the biological activities of the monoclonals suggest that the C and N terminals of VP85 are exposed at the surface of the viral particle.
Surleraux
M
M
Bodeus
M
M
Burtonboy
G
G
D000818:Animals; D000911:Antibodies, Monoclonal; D004285:Dogs / Q000382:microbiology*; D005455:Fluorescent Antibody Technique; D008854:Microscopy, Electron; D009500:Neutralization Tests; D010321:Parvoviridae / Q000032:analysis*; D010455:Peptides / Q000032:analysis; D014764:Viral Proteins / Q000032:analysis*
D000911:Antibodies, Monoclonal; D010455:Peptides; D014764:Viral Proteins