[Staining of leiomyoma tissue and myoma-derived cultured cells by the enzyme-labelled antibody method--using desmin, vimentin and fibronectin].
To elucidate the pathogenesis of leiomyoma, myoma tissues and myoma-derived cultured cells were stained with desmin, vimentin and fibronectin by the enzyme-labelled antibody method. Desmin staining was positive in both the myoma tissues and the cultured cells. Vimentin staining was positive in endothelial cells in the blood vessels of the myoma and in the cultured cells. Fibronectin staining was positive in both the myoma and cultured cells. These findings demonstrate that the leiomyoma was derived from myogenic cells. It was suggested that the myoma-derived cultured cells were myofibroblasts with myoblasts and fibroblasts, and had the ability to differentiate. It appears that the original cells of the myoma differentiate to myofibroblasts, which then differentiate into myoblasts and fibroblasts.
Otsuka
H
H
Yoshida
K
K
Kusano
S
S
Kashimura
M
M
Okamura
Y
Y
D000328:Adult; D002478:Cells, Cultured; D003893:Desmin / Q000032:analysis*; D005260:Female; D005353:Fibronectins / Q000032:analysis*; D006801:Humans; D007124:Immunoenzyme Techniques; D007889:Leiomyoma / Q000032:analysis*; D008875:Middle Aged; D014594:Uterine Neoplasms / Q000032:analysis*; D014746:Vimentin / Q000032:analysis*
D003893:Desmin; D005353:Fibronectins; D014746:Vimentin