Quantification of the bacterium Bacteroides gingivalis in human dental plaque by detection of the trypsin-like protease activity of colonies imprinted on membrane filters.
Individual colonies of this organism in primary cultures of human dental plaque were distinguished from colonies of other species of black-pigmented Bacteroides by detection of their trypsin-like proteolytic activity using a specific chromogenic enzyme substrate.
Tippet
B
B
Department of Microbiology, University of Otago, Dunedin, New Zealand.
Frisken
K W
KW
Tagg
J R
JR
D001439:Bacteroides / Q000201:enzymology / Q000302:isolation & purification*; D003773:Dental Plaque / Q000382:microbiology*; D006801:Humans; D010447:Peptide Hydrolases / Q000378:metabolism*
D010447:Peptide Hydrolases