title: Bone marrow cells other than stem cells seed the bone marrow after rescue transfusion of fatally irradiated mice.
abstract: In a previous publication, iodinated deoxyuridine (125IUdR) incorporation data were interpreted as indicating that spleen colony-forming units (CFU-S) in DNA synthesis preferentially seeded bone marrow. In the present studies, the CFU-S content of marrow from irradiated, bone-marrow transfused mice was directly determined. Pretreatment of the transfused cells with cytocidal tritiated thymidine resulted in an insignificant diminution in CFU-S content when compared with nontritiated thymidine pretreatment, implying that there is no preferential seeding. The 125IUdR incorporation data have been reinterpreted as being a result of the proliferation of other progenitor cells present that have seeded the bone marrow.
authors.affiliation: Medical Department, Brookhaven National Laboratory, Upton, New York 11973.
authors.forename: E P
authors.initials: EP
authors.lastname: Cronkite
authors.forename: T
authors.initials: T
authors.lastname: Inoue
authors.forename: J E
authors.initials: JE
authors.lastname: Bullis
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000528:radiation effects*; D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation; D003114:Colony-Forming Units Assay; D004261:DNA Replication / Q000528:radiation effects*; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000528:radiation effects*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D013936:Thymidine / Q000378:metabolism
chemical_list: D013936:Thymidine