The substitution of glucose-antiglucose oxidase complex (GAG) for peroxidase-antiperoxidase (PAP) in immunohistochemical studies of skin.
The substitution of glucose-antiglucose oxidase complex (GAG) for peroxidase antiperoxidase (PAP) in the Sternberger three-layer unlabeled antibody method avoids the problems associated with the use of the chromogen 3,3,diaminobenzidine (DAB). The blue final reaction product obtained using the GAG complex is not obscured by melanin granules, which is often the case when using the PAP-DAB combination. The replacement of PAP with GAG complexes facilitates the detection of melanocytes in melanoma and blue nevi. The absence of endogenous glucose oxidase in mammals provides for greater image contrast, which is not possible using alternative chromogens and immunoperoxidase.
Elias
J M
JM
Phillips
M E
ME
Boss
E
E
Merot
Y
Y
Mihm
M C
MC
D005949:Glucose Oxidase / Q000037:antagonists & inhibitors; D006801:Humans; D007124:Immunoenzyme Techniques; D008545:Melanoma / Q000032:analysis*; D009508:Nevus, Pigmented / Q000032:analysis*; D009418:S100 Proteins / Q000032:analysis*; D012867:Skin / Q000032:analysis*
D009418:S100 Proteins; D005949:Glucose Oxidase