title: The distribution and ontogeny of MHC antigens in murine ocular tissue.
abstract: The distribution of H-2 (Class I) and Ia (Class II) antigens in the mouse eye was determined with the use of monoclonal antibodies and found to be different. H-2 antigen was observed in the corneal epithelium, conjunctival epithelium, choroid, and inner and outer nuclear layer of the retina--in order of decreased intensity of staining. In contrast, Ia was detected most strongly, in a patchy distribution, in the choroid, limbus epithelium, and peripheral corneal stroma, followed by the iris and ciliary body. Thus, distinctively different patterns of distribution of H-2 and Ia were observed in the normal mouse eye. Surprisingly, adult eyes stained more intensely for both H-2 and Ia antigens than neonatal eyes, implying that the expression of MHC antigens varies with ontogeny. Since unique and important immunologic functions have been ascribed to class I and II antigens, their different distribution within the eye may indicate that various ocular structures can play distinctive roles in the immune response.
authors.forename: H M
authors.initials: HM
authors.lastname: Wang
authors.forename: H J
authors.initials: HJ
authors.lastname: Kaplan
authors.forename: W C
authors.initials: WC
authors.lastname: Chan
authors.forename: M
authors.initials: M
authors.lastname: Johnson
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D002829:Choroid / Q000276:immunology; D002924:Ciliary Body / Q000276:immunology; D003315:Cornea / Q000276:immunology; D005123:Eye / Q000276:immunology*; D006183:H-2 Antigens / Q000032:analysis*; D000949:Histocompatibility Antigens Class II / Q000032:analysis*; D007124:Immunoenzyme Techniques; D007498:Iris / Q000276:immunology; D051379:Mice; D012160:Retina / Q000276:immunology; D014018:Tissue Distribution
chemical_list: D000911:Antibodies, Monoclonal; D006183:H-2 Antigens; D000949:Histocompatibility Antigens Class II