title: Ultrastructural and immunocytochemical characterization of polymorphonuclear leukocytes from gingival crevice in man.
abstract: Polymorphonuclear leukocytes from the gingival crevicular fluid (CF-PMNs) of patients with generalized severe periodontitis were examined using electron microscopy and immunocytochemical techniques. CF-PMNs were found to contain numerous phagocytic vacuoles. This suggests that CF-PMNs actively phagocytized various substances from the environment. Immunocytochemical staining with FITC-conjugated IgG, IgM, and IgA reagents and TRITC-conjugated C3 reagent was applied to CF-PMNs as well as peripheral blood PMNs incubated with cell-free crevicular fluid. The cytoplasm of PMNs exhibited numerous granular foci of immunofluorescence. This finding suggests that these proteins were acquired from the environment by PMNs. The coincidental appearances of immunoglobulins and C3 in a single cell were considered to be immune complexes phagocytized by CF-PMNs in generalized severe periodontitis.
authors.forename: I
authors.initials: I
authors.lastname: Saito
authors.forename: K
authors.initials: K
authors.lastname: Komiyama
authors.forename: I
authors.initials: I
authors.lastname: Moro
authors.forename: K
authors.initials: K
authors.lastname: Akachi
authors.forename: N
authors.initials: N
authors.lastname: Shiomi
authors.forename: K
authors.initials: K
authors.lastname: Ito
authors.forename: S
authors.initials: S
authors.lastname: Murai
authors.forename: S
authors.initials: S
authors.lastname: Umemura
mesh_terms: D000328:Adult; D002452:Cell Count; D003165:Complement System Proteins / Q000032:analysis; D005883:Gingival Crevicular Fluid / Q000276:immunology*; D005891:Gingivitis / Q000276:immunology*; D006801:Humans; D007136:Immunoglobulins / Q000032:analysis*; D008875:Middle Aged; D009504:Neutrophils / Q000276:immunology / Q000648:ultrastructure*; D010518:Periodontitis / Q000276:immunology / Q000473:pathology*
chemical_list: D007136:Immunoglobulins; D003165:Complement System Proteins