Transplanted isolated hepatocytes: effect of partial hepatectomy on proliferation of long-term syngeneic implants in rat spleen.
Proliferation of isolated hepatocytes in long-term splenic implants was assessed by flash labelling with a 1 pulse of tritiated thymidine (3H TdR). Cell kinetics showed that the basal labelling index was 0.9% which was greater than normal non-regenerating liver. Twenty four hours following partial hepatectomy the labelling index was 2.0%, a significant rise. These results suggest that hepatocytes transplanted to the spleen constitute a suitable model for screening putative hepatotrophic factors, and are of relevance in establishing clinically useful models of hepatocyte transplantation.
Gupta
S
S
Johnstone
R
R
Darby
H
H
Selden
C
C
Price
Y
Y
Hodgson
H J
HJ
D000818:Animals; D002455:Cell Division; D006498:Hepatectomy; D008099:Liver / Q000166:cytology; D016031:Liver Transplantation; D051381:Rats; D011919:Rats, Inbred Strains; D013154:Spleen / Q000601:surgery*; D013997:Time Factors; D014185:Transplantation, Isogeneic