title: Fourier transform infrared investigation of the Escherichia coli methionine aporepressor.
abstract: This study represents the first physicochemical analysis of the recently cloned methionine repressor protein (Met aporepressor) from Escherichia coli. Infrared spectrometry was used to investigate the secondary structure and the hydrogen-deuterium exchange behavior of the E. coli Met aporepressor. The secondary structure of the native bacterial protein was derived by analysis of the amide I mode. The amide I band contour was found to consist of five major component bands (at 1625, 1639, 1653, 1665, and 1676 cm-1) which reflect the presence of various substructures. The relative areas of these component bands are consistent with a high alpha-helical content of the peptide chain secondary structure in solution (43%) and a small amount of beta-sheet structure (7%). The remaining substructure is assigned to turns (10%) and to unordered (or less ordered) structures (40%). The temperature dependence of the infrared spectra of native Met aporepressor in D2O medium over the temperature interval 20-80 degrees C indicates that there are two discrete thermal events: the first thermal event, centered at 42 degrees C, is associated with the hydrogen-deuterium exchange of the hard-to-exchange alpha-helical peptide bonds accompanied by a partial denaturation of the protein, while the second event, centered around 50 degrees C, represents the irreversible thermal denaturation of the protein.
authors.forename: P W
authors.initials: PW
authors.lastname: Yang
authors.forename: H H
authors.initials: HH
authors.lastname: Mantsch
authors.forename: J L
authors.initials: JL
authors.lastname: Arrondo
authors.forename: I
authors.initials: I
authors.lastname: Saint-Girons
authors.forename: Y
authors.initials: Y
authors.lastname: Guillou
authors.forename: G N
authors.initials: GN
authors.lastname: Cohen
authors.forename: O
authors.initials: O
authors.lastname: Bârzu
mesh_terms: D001059:Apoproteins; D004926:Escherichia coli / Q000378:metabolism*; D029968:Escherichia coli Proteins; D005583:Fourier Analysis; D011487:Protein Conformation; D012097:Repressor Proteins; D013055:Spectrophotometry, Infrared; D013816:Thermodynamics; D014157:Transcription Factors
chemical_list: D001059:Apoproteins; D029968:Escherichia coli Proteins; C053317:MetJ protein, E coli; D012097:Repressor Proteins; D014157:Transcription Factors