Use of radiolabeled monoclonal anti-B1 antibody for B lymphocyte imaging in rhesus monkeys.
Imaging tissues rich in B lymphocytes in man using a radiolabeled monoclonal anti-B cell antibody would be extremely useful in the clinical staging of non-Hodgkins lymphomas. Studies were done in rhesus monkeys using radiolabeled monoclonal anti-B1 antibody to determine the feasibility of such an approach. Immunohistologic studies demonstrated that infused monoclonal anti-B1 binds in vivo with specificity to B cells in lymph nodes and spleen. The kinetics of clearance of 131I-labeled anti-B1 were determined. The B lymphocyte-rich spleen could be readily visualized by gamma camera scanning without significant background and without the need for image intensification or blood background subtraction techniques. These data support the feasibility of using anti-B1 for staging B cell lymphomas in man.
Letvin
N L
NL
Zalutsky
M R
MR
Lambert
J M
JM
Chalifoux
L V
LV
Nadler
L M
LM
Schlossman
S F
SF
Atkins
H L
HL
D000818:Animals; D000911:Antibodies, Monoclonal; D001402:B-Lymphocytes / Q000000981:diagnostic imaging*; D006207:Half-Life; D007124:Immunoenzyme Techniques; D007454:Iodides / Q000097:blood; D007457:Iodine Radioisotopes; D008198:Lymph Nodes / Q000000981:diagnostic imaging; D008253:Macaca mulatta; D011877:Radionuclide Imaging
D000911:Antibodies, Monoclonal; D007454:Iodides; D007457:Iodine Radioisotopes