title: A comparative study of immuno-blotting techniques for the detection of Gc-subtypes after isoelectric focusing on agarose and polyacrylamide gels.
abstract: A comparison of separation and detection techniques has been carried out to determine the most suitable combination for use in Gc-subtyping. The best results (i.e., high sensitivity, distinct bands, especially with reference to the 1S and 1F separation) were achieved using isoelectric focusing in polyacrylamide gel (pH 4.5-5.4) followed by transfer to nitrocellulose membrane by electroblotting and finally detection with enzyme-linked antibody complex.
authors.forename: S
authors.initials: S
authors.lastname: Rand
authors.forename: P
authors.initials: P
authors.lastname: Ritter
authors.forename: A
authors.initials: A
authors.lastname: Kohfahl
authors.forename: B
authors.initials: B
authors.lastname: Brinkmann
mesh_terms: D004586:Electrophoresis; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007124:Immunoenzyme Techniques; D007158:Immunologic Techniques; D007525:Isoelectric Focusing; D014809:Vitamin D-Binding Protein / Q000235:genetics*
chemical_list: D014809:Vitamin D-Binding Protein