Immunological characterization of chromogranins A and B and secretogranin II in the bovine pancreatic islet.
Antisera against chromogranin A and B and secretogranin II were used for analysing the bovine pancreas by immunoblotting and immunohistochemistry. All three antigens were found in extracts of fetal pancreas by one dimensional immunoblotting. A comparison with the soluble proteins of chromaffin granules revealed that in adrenal medulla and in pancreas antigens which migrated identically in electrophoresis were present. In immunohistochemistry, chromogranin A was found in all pancreatic endocrine cell types with the exception of most pancreatic polypeptide-(PP-) producing cells. For chromogranin B, only a faint immunostaining was obtained. For secretogranin II, A- and B-cells were faintly positive, whereas the majority of PP-cells exhibited a strong immunostaining for this antigen. These results establish that chromogranins A and B and secretogranin II are present in the endocrine pancreas, but that they exhibit a distinct cellular localization.
Yoshie
S
S
Hagn
C
C
Ehrhart
M
M
Fischer-Colbrie
R
R
Grube
D
D
Winkler
H
H
Gratzl
M
M
D000313:Adrenal Medulla / Q000032:analysis; D000818:Animals; D002417:Cattle; D053379:Chromogranin A; D002864:Chromogranins / Q000032:analysis*; D006651:Histocytochemistry; D007158:Immunologic Techniques; D007515:Islets of Langerhans / Q000032:analysis*; D009419:Nerve Tissue Proteins / Q000032:analysis*; D011506:Proteins / Q000032:analysis*
D053379:Chromogranin A; D002864:Chromogranins; D009419:Nerve Tissue Proteins; D011506:Proteins