Alteration of the microtubule organization in aging WI-38 fibroblasts. A comparative study with embryonic hamster lung fibroblasts.
The microtubule organization in human WI-38 fibroblasts subcultivated in vitro has been investigated using nocodazole, a reversible inhibitor of the microtubules. Two phenotypes were observed. The typical fibroblast cells, called Type 1 cells, showed, after nocodazole treatment, a centripetal depolymerization wave of the microtubules and the giant Type 2 cells which have a more heterogeneous behaviour. Some of the cells clearly showed a centrifugal depolymerization of the microtubules, others a mixed behavior and less than 1% displayed the same behavior as the Type 1 cells. Confirming previous data obtained with Hamster fibroblasts (Raes et al., 1983, 1984), these results suggest a modification in the microtubule organization which could account for the aberrant division of some WI-38 cells in aged cultures. The relevance of this observation for the emergence of the morphologically different Type 2 cells and for cell division impairment in serially in vitro cultivated cells is discussed.
Raes
M
M
Remacle
J
J
D000375:Aging; D000818:Animals; D001562:Benzimidazoles / Q000494:pharmacology; D002452:Cell Count; D002460:Cell Line; D006224:Cricetinae; D004247:DNA / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism / Q000648:ultrastructure; D007700:Kinetics; D008168:Lung / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure*; D008870:Microtubules / Q000378:metabolism / Q000648:ultrastructure*; D015739:Nocodazole; D011108:Polymers / Q000378:metabolism
D001562:Benzimidazoles; D011108:Polymers; D004247:DNA; D015739:Nocodazole