[Isolation of monoclonal antibodies to horseradish peroxidase and their use in immunohistochemistry and immunoblotting].
Monoclonal anti-peroxidase antibodies (McAb) were generated by means of hybridization of BALB/c immune splenocytes with X-63.653 cells. Peroxidase-antiperoxidase (PAP) staining of fibroblast cultures with murine McAb against fibronectin was used for the detection of positive cultures. McAb AP-FC-2B4 were found to be highly efficient in different immunoenzyme tests, e.g., immunochemistry and immunoblotting. PAP method was used for histological diagnosis of invasive human breast cancer.
Faerman
A I
AI
Chervonskiĭ
A V
AV
Chipysheva
T A
TA
Gel'shteĭn
V I
VI
Koliada
A Iu
AIu
D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D001940:Breast / Q000378:metabolism; D001943:Breast Neoplasms / Q000378:metabolism; D005069:Evaluation Studies as Topic; D005260:Female; D006651:Histocytochemistry; D006735:Horseradish Peroxidase / Q000276:immunology*; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007124:Immunoenzyme Techniques; D007158:Immunologic Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D008862:Microsomes, Liver / Q000378:metabolism; D010544:Peroxidases / Q000276:immunology*; D051381:Rats
D000911:Antibodies, Monoclonal; D006735:Horseradish Peroxidase; D010544:Peroxidases
Poluchenie monoklonal'nykh antitel k peroksidaze khrena i ikh primenenie v immunogistokhimii i immunoblottinge.