title: Canine bronchoconstriction, gas trapping, and hypoxia with methacholine.
abstract: The effects of an intravenous methacholine infusion on cardiovascular-pulmonary function were measured in seven mongrel dogs (22.0 +/- 2.8 kg), anesthetized with chloralose and urethan and beta-adrenergically blocked with propranolol. In a volume-displacement plethysmograph, physiological measurements were made at base line and 25 min after establishing a methacholine infusion (0.1-1.0 mg X kg-1 X h-1). Methacholine significantly (P less than 0.05) increased airways resistance (1.9 +/- 0.8 to 8.2 +/- 2.9 cmH2O X l-1 X s), decreased static lung compliance (84.7 +/- 18.5 to 48.2 +/- 9.4 ml/cmH2O), depressed arterial PO2 (81 +/- 17 to 56 +/- 10 Torr), and lowered blood pressure (132 +/- 10 to 69 +/- 18 Torr) and cardiac output (5.7 +/- 1.9 to 4.1 +/- 1.2 l/min). These effects persisted during a further 80 min of methacholine infusion conducted in five of the animals. During the initial 25-min period of methacholine, the end-expired volume (volume-displacement Krogh spirometer) rose in all animals, indicating an increase in functional residual capacity from 997 +/- 115 to 1,623 +/- 259 ml (P less than 0.0005). Analysis of pulmonary pressure-volume curves revealed no change in total lung capacity but an increase in residual volume from 489 +/- 168 to 1,106 +/- 216 ml (P less than 0.001). Thus methacholine caused 617 ml of gas trapping, which was not detected by the Boyle's law principle, presumably because gas was trapped at high transpulmonary pressure. We suggest that intravenous methacholine-induced canine bronchoconstriction, which causes gas trapping and hypoxia, may be a useful animal model of clinical status asthmaticus.
authors.forename: P H
authors.initials: PH
authors.lastname: Breen
authors.forename: L J
authors.initials: LJ
authors.lastname: Becker
authors.forename: P
authors.initials: P
authors.lastname: Ruygrok
authors.forename: I
authors.initials: I
authors.lastname: Mayers
authors.forename: G R
authors.initials: GR
authors.lastname: Long
authors.forename: A
authors.initials: A
authors.lastname: Leff
authors.forename: L D
authors.initials: LD
authors.lastname: Wood
mesh_terms: D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D002302:Cardiac Output / Q000187:drug effects; D004285:Dogs; D000860:Hypoxia / Q000503:physiopathology*; D008168:Lung / Q000187:drug effects / Q000502:physiology* / Q000503:physiopathology; D008170:Lung Compliance / Q000187:drug effects; D008176:Lung Volume Measurements; D016210:Methacholine Chloride; D008688:Methacholine Compounds / Q000494:pharmacology*; D010100:Oxygen / Q000097:blood; D010313:Partial Pressure; D012119:Respiration / Q000187:drug effects*; D013990:Tidal Volume; D014797:Vital Capacity / Q000187:drug effects
chemical_list: D008688:Methacholine Compounds; D016210:Methacholine Chloride; D010100:Oxygen