Isolation and purification of a major allergen from Parietaria officinalis pollen.
A major allergen of Parietaria officinalis, a species responsible for a large number of respiratory allergies in Mediterranean areas, has been identified and characterized. This allergen (Pol) was found in the fraction which precipitates between 70 and 100% ammonium sulphate saturation. Pol showed a molecular weight of 15,000 daltons as determined by SDS-PAGE and HPLC. The pI of Pol was in the pH region 4-6, IEF showing four major bands. Two major bands were shown by CIE, CRIE and immunoblotting; major contaminants or aggregates were also revealed by the latter technique and by HPLC. Pol showed an allergic specific activity 2 times higher than the crude extract; moreover it was shown to be a major allergen since it inhibited 29 out of 30 sera from allergic patients sensitive to P. officinalis.
Giuliani
A
A
Laboratorio di Immunologia, Università La Sapienza, Roma, Italy.
Pini
C
C
Bonini
S
S
Mucci
N
N
Ferroni
L
L
Vicari
G
G
D000485:Allergens / Q000276:immunology / Q000302:isolation & purification*; D000818:Animals; D000917:Antibody Formation; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D007158:Immunologic Techniques; D008970:Molecular Weight; D010940:Plant Proteins / Q000276:immunology / Q000302:isolation & purification*; D006109:Poaceae / Q000032:analysis / Q000276:immunology; D011058:Pollen / Q000032:analysis* / Q000276:immunology; D011817:Rabbits
D000485:Allergens; D010940:Plant Proteins