title: Comparison of the tertiary structure of yeast tRNA(Asp) and tRNA(Phe) in solution. Chemical modification study of the bases.
abstract: A comparative study of the solution structures of yeast tRNA(Asp) and tRNA(Phe) was undertaken with chemical reagents as structural probes. The reactivity of N-7 positions in guanine and adenine residues was assayed with dimethylsulphate and diethyl-pyrocarbonate, respectively, and that of the N-3 position in cytosine residues with dimethylsulphate. Experiments involved statistical modifications of end-labelled tRNAs, followed by splitting at modified positions. The resulting end-labelled oligonucleotides were resolved on polyacrylamide sequencing gels and analysed by autoradiography. Three different experimental conditions were used to follow the progressive denaturation of the two tRNAs. Experiments were done in parallel on tRNA(Asp) and tRNA(Phe) to enable comparison between the two solution structures and to correlate the results with the crystalline conformations of both molecules. Structural differences were detected for G4, G45, G71 and A21: G4 and A21 are reactive in tRNA(Asp) and protected in tRNA(Phe), while G45 and G71 are protected in tRNA(Asp) and reactive in tRNA(Phe). For the N-7 atom of A21, the different reactivity is correlated with the variable variable loop structures in the two tRNAs; in the case of G45 the results are explained by a different stacking of A9 between G45 and residue 46. For G4 and G71, the differential reactivities are linked to a different stacking in both tRNAs. This observation is of general significance for helical stems. If the previous results could be fully explained by the crystal structures, unexpected similarities in solution were found for N-3 alkylation of C56 in the T-loop, which according to crystallography should be reactive in tRNA(Asp). The apparent discrepancy is due to conformational differences between crystalline and solution tRNA(Asp) at the level of the D and T-loop contacts, linked to long-distance effects induced by the quasi-self-complementary anticodon GUC, which favour duplex formation within the crystal, contrarily to solution conditions where the tRNA is essentially in its free state.
authors.affiliation: Institut de Biologie Moléculaire et Cellulaire, Centre de le Recherche Scientifique, Strasbourg, France.
authors.forename: P
authors.initials: P
authors.lastname: Romby
authors.forename: D
authors.initials: D
authors.lastname: Moras
authors.forename: P
authors.initials: P
authors.lastname: Dumas
authors.forename: J P
authors.initials: JP
authors.lastname: Ebel
authors.forename: R
authors.initials: R
authors.lastname: Giegé
mesh_terms: D000477:Alkylating Agents; D000478:Alkylation; D001483:Base Sequence; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D012331:RNA, Fungal; D012346:RNA, Transfer, Amino Acyl; D012441:Saccharomyces cerevisiae / Q000032:analysis*; D013463:Sulfuric Acid Esters
chemical_list: D000477:Alkylating Agents; D012331:RNA, Fungal; D012346:RNA, Transfer, Amino Acyl; D013463:Sulfuric Acid Esters; C007482:dimethyl sulfate