Thermally modified azocasein--a new insoluble substrate for the determination of proteolytic activity.
An insoluble chromogenic substrate for the determination of proteolytic activity was prepared by heating azocasein in a thin layer at 200 degrees C for 4 h in a hot-air thermostat. The activity of an extracellular bacterial proteinase produced by Bacillus sp. was determined with this new substrate.
Safarík
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I
Laboratory of Analytical Chemistry, Czechoslovak Academy of Sciences, Ceské Budĕjovice.
D002364:Caseins; D006358:Hot Temperature; D007700:Kinetics; D010447:Peptide Hydrolases / Q000378:metabolism*; D013053:Spectrophotometry / Q000379:methods
D002364:Caseins; C026018:azocasein; D010447:Peptide Hydrolases