Cholesteryl ester transfer protein is secreted by Hep G2 cells and contains asparagine-linked carbohydrate and sialic acid.
A cholesteryl ester transfer protein (CETP) of apparent Mr 74,000 has recently been purified from human plasma. Cholesteryl ester transfer activity was found to accumulate in the medium of cultured Hep G2 cells. The transfer activity was removed by immunoprecipitation with specific antibodies to the plasma CETP. Sodium dodecyl sulfate gel electrophoresis of immunoprecipitates prepared from the medium of cells pulsed with [35S]methionine revealed a broad specific band of protein of Mr 72,000 to 76,000; by contrast, immunoprecipitates of cellular homogenates showed a sharp specific band of Mr 58,000. The Mr 72,000 to 76,000 band disappears, concomitant with the appearance of lower Mr products, upon neuraminidase or glycopeptidase F treatment of medium immunoprecipitates or of purified CETP. The results indicate that liver cells have the capacity to synthesize and secrete CETP. The CETP peptide acquires asparagine-linked carbohydrate and sialic acid during intracellular processing.
Swenson
T L
TL
Department of Medicine, Columbia University College of Physicians and Surgeons, New York, New York 10032.
Simmons
J S
JS
Hesler
C B
CB
Bisgaier
C
C
Tall
A R
AR
D000818:Animals; D001216:Asparagine / Q000032:analysis*; D002241:Carbohydrates / Q000032:analysis*; D002352:Carrier Proteins / Q000378:metabolism*; D002460:Cell Line; D053480:Cholesterol Ester Transfer Proteins; D006023:Glycoproteins; D006026:Glycoside Hydrolases / Q000378:metabolism; D007163:Immunosorbent Techniques; D008114:Liver Neoplasms, Experimental / Q000378:metabolism*; D008970:Molecular Weight; D019158:N-Acetylneuraminic Acid; D012794:Sialic Acids / Q000032:analysis*
C506642:CETP protein, human; D002241:Carbohydrates; D002352:Carrier Proteins; D053480:Cholesterol Ester Transfer Proteins; D006023:Glycoproteins; D012794:Sialic Acids; D001216:Asparagine; D006026:Glycoside Hydrolases; D019158:N-Acetylneuraminic Acid