Chlorophyll-protein composition of the thylakoid membrane from Prochlorothrix hollandica, a prokaryote containing chlorophyll b.
The chlorophyll-protein complexes of the thylakoid membrane from Prochlorothrix hollandica were identified following electrophoresis under nondenaturing conditions. Five complexes, CP1-CP5, were resolved and these green bands were analyzed by spectroscopic and immunological methods. CP1 contains the photosystem I (PSI) reaction center, as this complex quenched fluorescence at room temperature, and had a 77 K fluorescence emission peak at 717 nm. CP4 contains the major chlorophyll-a-binding proteins of the photosystem II (PSII) core, because this complex contained polypeptides which cross-reacted to antibodies raised against Chlamydomonas PSII proteins 5 and 6. Furthermore, fluorescence excitation studies at 77 K indicated that only a Chl a is bound to CP4. Complexes CP2, CP3 and CP5 contained functionally bound Chl a and b as judged by absorption spectroscopy at 20 degrees C and fluorescence excitation spectra at 77 K. CP2, CP3 and CP5 all contain polypeptides of 30-33 kDa which are immunologically distinct from the LHC-II complex of higher plant thylakoids.
Bullerjahn
G S
GS
Division of Biological Sciences, University of Missouri, Columbia 65211.
Matthijs
H C
HC
Mur
L R
LR
Sherman
L A
LA
D002734:Chlorophyll / Q000032:analysis*; D000077194:Chlorophyll A; D002736:Chloroplasts / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D007118:Immunoassay; D045342:Light-Harvesting Protein Complexes; D010455:Peptides / Q000032:analysis; D045322:Photosynthetic Reaction Center Complex Proteins; D045331:Photosystem I Protein Complex; D045332:Photosystem II Protein Complex; D010860:Pigments, Biological / Q000032:analysis; D010940:Plant Proteins / Q000032:analysis*; D011485:Protein Binding; D011489:Protein Denaturation; D013050:Spectrometry, Fluorescence
D045342:Light-Harvesting Protein Complexes; D010455:Peptides; D045322:Photosynthetic Reaction Center Complex Proteins; D045331:Photosystem I Protein Complex; D045332:Photosystem II Protein Complex; D010860:Pigments, Biological; D010940:Plant Proteins; D002734:Chlorophyll; C037184:chlorophyll b; D000077194:Chlorophyll A