[Radioimmunological assay of plasma arginine-vasopressin in man. Comparison of 2 methods of extraction].
Two methods of extraction, prior to radio-immunoassay (RIA) of human plasma arginine vasopressin (AVP) were tested: ethanol extraction (ETH), chromatography with ODS-Silica (ODS-Sil). Recovery of 125I AVP was higher when using ODS-Sil than when using ETH. Recovery of standard AVP and plasma enriched with standard AVP was found to be more efficient and reproducible for ODS-Sil. However, the RIA used, performed after chromatography with ODS-Sil, is not enough sensitive to detect low concentrations but is able to detect high concentrations and physiological variations of plasma AVP.
Wolf
J P
JP
Dumoulin
G
G
Henriet
M T
MT
Baulay
A
A
Berthelay
S
S
D001127:Arginine Vasopressin / Q000097:blood* / Q000302:isolation & purification; D002845:Chromatography; D000431:Ethanol; D006801:Humans; D011863:Radioimmunoassay
D001127:Arginine Vasopressin; D000431:Ethanol
Dosage radioimmunologique de l'arginine-vasopressine plasmatique chez l'homme. Comparaison de deux méthodes d'extraction.